LOCUS       DAMJWU010000001       144750 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMJWU010000001 DAMJWU010000000
VERSION     DAMJWU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 144750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 144750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..144750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.001"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(772..1281)
                     /locus_tag="PC460_000001"
     CDS             complement(772..1281)
                     /locus_tag="PC460_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2598911.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
     gene            complement(1492..2901)
                     /locus_tag="PC460_000002"
     CDS             complement(1492..2901)
                     /locus_tag="PC460_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2598912.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            complement(2905..4035)
                     /locus_tag="PC460_000003"
     CDS             complement(2905..4035)
                     /locus_tag="PC460_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2598913.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            complement(4047..4850)
                     /locus_tag="PC460_000004"
     CDS             complement(4047..4850)
                     /locus_tag="PC460_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2598914.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            complement(5232..6113)
                     /locus_tag="PC460_000005"
     CDS             complement(5232..6113)
                     /locus_tag="PC460_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF2598915.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            6569..7543
                     /locus_tag="PC460_000006"
     CDS             6569..7543
                     /locus_tag="PC460_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2598916.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQILRDNNFYK"
     gene            complement(7659..8720)
                     /locus_tag="PC460_000007"
     CDS             complement(7659..8720)
                     /locus_tag="PC460_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2598917.1"
                     /translation="MKKIRKLTTKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(9332..10183)
                     /locus_tag="PC460_000008"
     CDS             complement(9332..10183)
                     /locus_tag="PC460_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2598918.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(10200..11120)
                     /locus_tag="PC460_000009"
     CDS             complement(10200..11120)
                     /locus_tag="PC460_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF2598919.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(11230..12144)
                     /gene="murQ"
                     /locus_tag="PC460_000010"
     CDS             complement(11230..12144)
                     /gene="murQ"
                     /locus_tag="PC460_000010"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF2598920.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(12243..13340)
                     /locus_tag="PC460_000011"
     CDS             complement(12243..13340)
                     /locus_tag="PC460_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2598921.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKRINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDIGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(13573..14919)
                     /locus_tag="PC460_000012"
     CDS             complement(13573..14919)
                     /locus_tag="PC460_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2598922.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPELLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(15008..15316)
                     /locus_tag="PC460_000013"
     CDS             complement(15008..15316)
                     /locus_tag="PC460_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2598923.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(15578..15976)
                     /locus_tag="PC460_000014"
     CDS             complement(15578..15976)
                     /locus_tag="PC460_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2598924.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(15978..17138)
                     /locus_tag="PC460_000015"
     CDS             complement(15978..17138)
                     /locus_tag="PC460_000015"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF2598925.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(17569..18588)
                     /locus_tag="PC460_000016"
     CDS             complement(17569..18588)
                     /locus_tag="PC460_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2598926.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSIDS"
     gene            complement(18582..19262)
                     /locus_tag="PC460_000017"
     CDS             complement(18582..19262)
                     /locus_tag="PC460_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2598927.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(19414..21321)
                     /locus_tag="PC460_000018"
     CDS             complement(19414..21321)
                     /locus_tag="PC460_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2598928.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(21322..22074)
                     /locus_tag="PC460_000019"
     CDS             complement(21322..22074)
                     /locus_tag="PC460_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2598929.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(22390..23544)
                     /locus_tag="PC460_000020"
     CDS             complement(22390..23544)
                     /locus_tag="PC460_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2598930.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(23547..24014)
                     /locus_tag="PC460_000021"
     CDS             complement(23547..24014)
                     /locus_tag="PC460_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2598931.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(24028..24516)
                     /locus_tag="PC460_000022"
     CDS             complement(24028..24516)
                     /locus_tag="PC460_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2598932.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(24530..25861)
                     /locus_tag="PC460_000023"
     CDS             complement(24530..25861)
                     /locus_tag="PC460_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2598933.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(25875..27440)
                     /locus_tag="PC460_000024"
     CDS             complement(25875..27440)
                     /locus_tag="PC460_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2598934.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(27610..28395)
                     /locus_tag="PC460_000025"
     CDS             complement(27610..28395)
                     /locus_tag="PC460_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2598935.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(28562..29761)
                     /locus_tag="PC460_000026"
     CDS             complement(28562..29761)
                     /locus_tag="PC460_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF2598936.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEEMSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(30283..31620)
                     /gene="xylA"
                     /locus_tag="PC460_000027"
     CDS             complement(30283..31620)
                     /gene="xylA"
                     /locus_tag="PC460_000027"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF2598937.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(31716..33266)
                     /gene="xylB"
                     /locus_tag="PC460_000028"
     CDS             complement(31716..33266)
                     /gene="xylB"
                     /locus_tag="PC460_000028"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF2598938.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            33474..34634
                     /locus_tag="PC460_000029"
     CDS             33474..34634
                     /locus_tag="PC460_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2598939.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(34790..35194)
                     /locus_tag="PC460_000030"
     CDS             complement(34790..35194)
                     /locus_tag="PC460_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2598940.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(35215..35697)
                     /locus_tag="PC460_000031"
     CDS             complement(35215..35697)
                     /locus_tag="PC460_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2598941.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(35717..36526)
                     /locus_tag="PC460_000032"
     CDS             complement(35717..36526)
                     /locus_tag="PC460_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2598942.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(36519..37298)
                     /locus_tag="PC460_000033"
     CDS             complement(36519..37298)
                     /locus_tag="PC460_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2598943.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(37298..39376)
                     /locus_tag="PC460_000034"
     CDS             complement(37298..39376)
                     /locus_tag="PC460_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF2598944.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(40175..40696)
                     /locus_tag="PC460_000035"
     CDS             complement(40175..40696)
                     /locus_tag="PC460_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2598945.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            40862..41428
                     /locus_tag="PC460_000036"
     CDS             40862..41428
                     /locus_tag="PC460_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2598946.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTIGTVLGAVV
                     LGPFWSALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(41645..43708)
                     /locus_tag="PC460_000037"
     CDS             complement(41645..43708)
                     /locus_tag="PC460_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF2598947.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(44089..47424)
                     /locus_tag="PC460_000038"
     CDS             complement(44089..47424)
                     /locus_tag="PC460_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2598948.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKTETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(47474..47902)
                     /locus_tag="PC460_000039"
     CDS             complement(47474..47902)
                     /locus_tag="PC460_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2598949.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(47892..48272)
                     /locus_tag="PC460_000040"
     CDS             complement(47892..48272)
                     /locus_tag="PC460_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF2598950.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYKSKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(48305..48613)
                     /locus_tag="PC460_000041"
     CDS             complement(48305..48613)
                     /locus_tag="PC460_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2598951.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(48658..49818)
                     /locus_tag="PC460_000042"
     CDS             complement(48658..49818)
                     /locus_tag="PC460_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2598952.1"
                     /translation="MEYRLNEVFEFIRNGASIKQFDTKFGYPITRIETIANSYIDTNK
                     MGYADIKELGKLEDYLLKNGDILMSHINSEKHLGKSAIYEDINKKIIHGMNLLCLRPD
                     KEVCYPKYLNYFFNSNEFRRQLPRIIKKSVNQASFSVNDLKNLKVDLPEMEAQKDISK
                     VLDKAQELIDKRKEQIEALDELVKSRFIEMFGNFKEQKFVLLKDCTNFIDYRGKTPIL
                     SENGIRIINAKSVGNGVFKYINEYISENTYNSWMKRGFPVAGDVLFVTEGHTFGNVCR
                     IPKDLKKFAMGQRIITIQGDKKILNNAFLAQYMQTKAFRVEIDKYKTGSSAQGIRSKE
                     LQKILIPVPSIELQNKFEEFVNQVDKLKVGIKTSLKELEDNFNSLMQKAFKG"
     gene            complement(49822..51285)
                     /locus_tag="PC460_000043"
     CDS             complement(49822..51285)
                     /locus_tag="PC460_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2598953.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            complement(51794..52663)
                     /gene="gatY"
                     /locus_tag="PC460_000044"
     CDS             complement(51794..52663)
                     /gene="gatY"
                     /locus_tag="PC460_000044"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2598954.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(52683..54599)
                     /locus_tag="PC460_000045"
     CDS             complement(52683..54599)
                     /locus_tag="PC460_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF2598955.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGL
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(54602..55522)
                     /locus_tag="PC460_000046"
     CDS             complement(54602..55522)
                     /locus_tag="PC460_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF2598956.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICENKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(55624..56376)
                     /locus_tag="PC460_000047"
     CDS             complement(55624..56376)
                     /locus_tag="PC460_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2598957.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTVNKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(56768..58213)
                     /locus_tag="PC460_000048"
     CDS             complement(56768..58213)
                     /locus_tag="PC460_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2598958.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKKRYKKDSFYWYQNVIRTNGEELKEK"
     gene            complement(58235..58588)
                     /locus_tag="PC460_000049"
     CDS             complement(58235..58588)
                     /locus_tag="PC460_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2598959.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(58617..59864)
                     /locus_tag="PC460_000050"
     CDS             complement(58617..59864)
                     /locus_tag="PC460_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2598960.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFTVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(59872..60189)
                     /locus_tag="PC460_000051"
     CDS             complement(59872..60189)
                     /locus_tag="PC460_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2598961.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            60431..62344
                     /locus_tag="PC460_000052"
     CDS             60431..62344
                     /locus_tag="PC460_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2598962.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     DLFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(62467..63621)
                     /locus_tag="PC460_000053"
     CDS             complement(62467..63621)
                     /locus_tag="PC460_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF2598963.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKDTIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(63881..66496)
                     /gene="mngB"
                     /locus_tag="PC460_000054"
     CDS             complement(63881..66496)
                     /gene="mngB"
                     /locus_tag="PC460_000054"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2598964.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(66640..68565)
                     /gene="mngA"
                     /locus_tag="PC460_000055"
     CDS             complement(66640..68565)
                     /gene="mngA"
                     /locus_tag="PC460_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF2598965.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AVLLIITRKQKNQRKIH"
     gene            complement(68695..69420)
                     /locus_tag="PC460_000056"
     CDS             complement(68695..69420)
                     /locus_tag="PC460_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2598966.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEVAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(69683..70453)
                     /locus_tag="PC460_000057"
     CDS             complement(69683..70453)
                     /locus_tag="PC460_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF2598967.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(70455..71882)
                     /locus_tag="PC460_000058"
     CDS             complement(70455..71882)
                     /locus_tag="PC460_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2598968.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            72273..72995
                     /locus_tag="PC460_000059"
     CDS             72273..72995
                     /locus_tag="PC460_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2598969.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            73192..74859
                     /locus_tag="PC460_000060"
     CDS             73192..74859
                     /locus_tag="PC460_000060"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2598970.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYYLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(74955..76274)
                     /locus_tag="PC460_000061"
     CDS             complement(74955..76274)
                     /locus_tag="PC460_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2598971.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRQTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(76315..77061)
                     /locus_tag="PC460_000062"
     CDS             complement(76315..77061)
                     /locus_tag="PC460_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2598972.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(77305..79314)
                     /locus_tag="PC460_000063"
     CDS             complement(77305..79314)
                     /locus_tag="PC460_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2598973.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(79708..81141)
                     /locus_tag="PC460_000064"
     CDS             complement(79708..81141)
                     /locus_tag="PC460_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2598974.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(81228..83144)
                     /locus_tag="PC460_000065"
     CDS             complement(81228..83144)
                     /locus_tag="PC460_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2598975.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGVFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(83218..84078)
                     /locus_tag="PC460_000066"
     CDS             complement(83218..84078)
                     /locus_tag="PC460_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2598976.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(84429..85616)
                     /locus_tag="PC460_000067"
     CDS             complement(84429..85616)
                     /locus_tag="PC460_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2598977.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(85630..87021)
                     /locus_tag="PC460_000068"
     CDS             complement(85630..87021)
                     /locus_tag="PC460_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2598978.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(87616..89316)
                     /locus_tag="PC460_000069"
     CDS             complement(87616..89316)
                     /locus_tag="PC460_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2598979.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(89288..90691)
                     /locus_tag="PC460_000070"
     CDS             complement(89288..90691)
                     /locus_tag="PC460_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2598980.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(90955..91446)
                     /locus_tag="PC460_000071"
     CDS             complement(90955..91446)
                     /locus_tag="PC460_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2598981.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(91472..92284)
                     /locus_tag="PC460_000072"
     CDS             complement(91472..92284)
                     /locus_tag="PC460_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2598982.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLSKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(92297..93448)
                     /locus_tag="PC460_000073"
     CDS             complement(92297..93448)
                     /locus_tag="PC460_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2598983.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            93718..94359
                     /locus_tag="PC460_000074"
     CDS             93718..94359
                     /locus_tag="PC460_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF2598984.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            94385..95929
                     /locus_tag="PC460_000075"
     CDS             94385..95929
                     /locus_tag="PC460_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2598985.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(96129..96755)
                     /locus_tag="PC460_000076"
     CDS             complement(96129..96755)
                     /locus_tag="PC460_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2598986.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(96890..97606)
                     /locus_tag="PC460_000077"
     CDS             complement(96890..97606)
                     /locus_tag="PC460_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF2598987.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDKAATKFGLE"
     gene            98032..99246
                     /locus_tag="PC460_000078"
     CDS             98032..99246
                     /locus_tag="PC460_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2598988.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            99866..101194
                     /locus_tag="PC460_000079"
     CDS             99866..101194
                     /locus_tag="PC460_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2598989.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(101322..102512)
                     /locus_tag="PC460_000080"
     CDS             complement(101322..102512)
                     /locus_tag="PC460_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2598990.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSKNGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQSVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQEYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(102618..103997)
                     /locus_tag="PC460_000081"
     CDS             complement(102618..103997)
                     /locus_tag="PC460_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2598991.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(104028..105236)
                     /locus_tag="PC460_000082"
     CDS             complement(104028..105236)
                     /locus_tag="PC460_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2598992.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIIPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            complement(106261..107715)
                     /locus_tag="PC460_000083"
     CDS             complement(106261..107715)
                     /locus_tag="PC460_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2598993.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKIGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            complement(107735..109606)
                     /locus_tag="PC460_000084"
     CDS             complement(107735..109606)
                     /locus_tag="PC460_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2598994.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(109596..110456)
                     /locus_tag="PC460_000085"
     CDS             complement(109596..110456)
                     /locus_tag="PC460_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2598995.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKASDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            complement(111051..111455)
                     /locus_tag="PC460_000086"
     CDS             complement(111051..111455)
                     /locus_tag="PC460_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2598996.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPLIYLKYKPDK"
     gene            complement(111647..112834)
                     /locus_tag="PC460_000087"
     CDS             complement(111647..112834)
                     /locus_tag="PC460_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF2598997.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(112866..113291)
                     /locus_tag="PC460_000088"
     CDS             complement(112866..113291)
                     /locus_tag="PC460_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2598998.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            113749..114498
                     /locus_tag="PC460_000089"
     CDS             113749..114498
                     /locus_tag="PC460_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2598999.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(114664..115029)
                     /locus_tag="PC460_000090"
     CDS             complement(114664..115029)
                     /locus_tag="PC460_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2599000.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(115045..115821)
                     /locus_tag="PC460_000091"
     CDS             complement(115045..115821)
                     /locus_tag="PC460_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2599001.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(116268..117695)
                     /gene="ascB"
                     /locus_tag="PC460_000092"
     CDS             complement(116268..117695)
                     /gene="ascB"
                     /locus_tag="PC460_000092"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2599002.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(117756..119684)
                     /locus_tag="PC460_000093"
     CDS             complement(117756..119684)
                     /locus_tag="PC460_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2599003.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(119829..120671)
                     /locus_tag="PC460_000094"
     CDS             complement(119829..120671)
                     /locus_tag="PC460_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2599004.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            complement(121018..122388)
                     /locus_tag="PC460_000095"
     CDS             complement(121018..122388)
                     /locus_tag="PC460_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2599005.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQDVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            complement(122427..123098)
                     /gene="pgmB"
                     /locus_tag="PC460_000096"
     CDS             complement(122427..123098)
                     /gene="pgmB"
                     /locus_tag="PC460_000096"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2599006.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            complement(123100..125490)
                     /locus_tag="PC460_000097"
     CDS             complement(123100..125490)
                     /locus_tag="PC460_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2599007.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVEDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSVGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            complement(125516..126964)
                     /locus_tag="PC460_000098"
     CDS             complement(125516..126964)
                     /locus_tag="PC460_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2599008.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMIIGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(127367..128254)
                     /locus_tag="PC460_000099"
     CDS             complement(127367..128254)
                     /locus_tag="PC460_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2599009.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(128354..128803)
                     /locus_tag="PC460_000100"
     CDS             complement(128354..128803)
                     /locus_tag="PC460_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2599010.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            complement(128793..130700)
                     /locus_tag="PC460_000101"
     CDS             complement(128793..130700)
                     /locus_tag="PC460_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2599011.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVSTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(130968..132863)
                     /locus_tag="PC460_000102"
     CDS             complement(130968..132863)
                     /locus_tag="PC460_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2599012.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(132899..133777)
                     /locus_tag="PC460_000103"
     CDS             complement(132899..133777)
                     /locus_tag="PC460_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF2599013.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(134482..135915)
                     /locus_tag="PC460_000104"
     CDS             complement(134482..135915)
                     /locus_tag="PC460_000104"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2599014.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPKGDESTPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     NRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNDSAGKA
                     DVVENPYLKISDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     DYRIEYLASHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            complement(135938..137779)
                     /locus_tag="PC460_000105"
     CDS             complement(135938..137779)
                     /locus_tag="PC460_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2599015.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLSANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDIISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDRIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            complement(137905..138738)
                     /locus_tag="PC460_000106"
     CDS             complement(137905..138738)
                     /locus_tag="PC460_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2599016.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGREELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKNIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            complement(139782..139994)
                     /locus_tag="PC460_000107"
     CDS             complement(139782..139994)
                     /locus_tag="PC460_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599017.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(139995..140411)
                     /locus_tag="PC460_000108"
     CDS             complement(139995..140411)
                     /locus_tag="PC460_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599018.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(140481..140894)
                     /locus_tag="PC460_000109"
     CDS             complement(140481..140894)
                     /locus_tag="PC460_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599019.1"
                     /translation="MSKSELGVKANLVSELMMLSYFFIDLLLQKFIFKGEGIWFTFII
                     LLAFWVCNLMYNKKYNDLVDEASKLMLLKVDQIASKIMLYSMFVIAVLFSVNHTRALD
                     ISNLDIGIMLLAVLFIQSLSKLILYIYFDKKGICN"
     gene            complement(141728..142357)
                     /locus_tag="PC460_000110"
     CDS             complement(141728..142357)
                     /locus_tag="PC460_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF2599020.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(142436..143398)
                     /locus_tag="PC460_000111"
     CDS             complement(142436..143398)
                     /locus_tag="PC460_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2599021.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(144160..>144750)
                     /locus_tag="PC460_000112"
     CDS             complement(144160..>144750)
                     /locus_tag="PC460_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599022.1"
                     /translation="DPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDN
                     GGNSKPDIDTDGDGKPNINIDIDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDI
                     DGDGKPDINIDIDGDGKPDINIDTDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQ
                     NVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT        47328 a        25529 c        15342 g        56551 t
ORIGIN      
        1 ttatcttaat tttctaaata taagtgaatg ctatcatttt aagtatctta taagcaatgt
       61 aactatatat ctctaataac tactttttta acatgcttaa actatttata agctattata
      121 tttatgtatt aacctaataa ctttttcact aaaaaataac aatctattca aagataaact
      181 atagtacttc ttctactttg tttcaataaa atactaaata aatttcgtac atccacaatt
      241 tttcaggcta cactataaat atacaatctt tatcttagtt ataagtttaa gaatattttt
      301 agtcttattt tgtcgaatat ctttttaata aaacttcata attctaagca tatttttaca
      361 ctgtgttttc atgtacaaaa ataatgctta caaataaaca acataaataa aacttaaata
      421 agatggattc tattcaacat agtatccatc ttattcattc aaaacctaaa aggatttttt
      481 ataatattat atattttaaa atctaagtaa ctcattatag tatcaatttc acttaaagct
      541 tacaatacac ttattaatct tgttttatat taaaaacatc tacaataaca atttaaatct
      601 ttaagtgata tccatctcac acctaaagga gtaggctctc cgcactattc tgtaattctt
      661 caatacaatc atcattattt tttatataat ttaattatat attttatttt accaaaacca
      721 gcatacataa ataatactta aaatagatta aataaaggat actaaaacac tttattgagg
      781 tatatgactt gaaatatatg aactaacttc attaggagaa atatctaaaa tagttgcaaa
      841 ttcttcattt aataaccttt cagcttcttt cattatatta tcatctgctt gatgtgtttt
      901 tttaccaata gattttacat actctctgtt attatctata cttcttacta attttattaa
      961 ctcttcacat ttaccacctc tgagtatttt tttaaattgc tcacttctca atttttcatt
     1021 atctatccat gatgtatcca tattaggaat atcatttatt aatgaaagca cattaatttt
     1081 agaaagtaac tttctcattg gtactttttc attatctaca ggaattttta ttactgtatt
     1141 attagaataa ataggactca atacataata ctcttttttt attccatttg taaatcgttc
     1201 atttgttata tccatgactt tgcagactcc tgtcatacca tacattatat aatcatcaat
     1261 cttaaacaaa aacatcacca tccttaataa aaaaaatagt tttgacaact aaatccacct
     1321 tgccaaaact atctgctata tattatattt taacacattt taagacttaa atgtaagtaa
     1381 aacttatatt attaaaatta aaaaatacat taaccattga ttaatgtatt ttttagattt
     1441 ttataacaag catatcaaaa taaattaata cagcttatct cctgtgaata cctataaaat
     1501 tggtgcttgg ctattcattg agtacccatc atttcttaca tcatttattt ctaatatttc
     1561 atcatttgtt ttaaccttag taagtttttt aactgctcca taaggttctt ttaatgaaat
     1621 tttatctcct cttccagaaa taacccataa aattttatac cctctaggta ttacttgtag
     1681 tttattttca cctttaccat cagtaaagta gactaacaaa ttaatctttt tattattagc
     1741 atactcaaag actggagaaa atctagtacc tccacctatc ctaattctat cttttatatc
     1801 tttttcagtt ctcactttat atacacgtct aatttcatta tcacattcta taattgtaat
     1861 ttcatgatta tagtttttca ctatactaag aacttcttta atagcttgtt taaactcttc
     1921 atcactaata cttccactta catcaagtgc aactgctatt tctgacttat gacttctaag
     1981 ttctcccctc aaatctaaac gatttggttg tcttctattt cttcttgtta tagtcttctt
     2041 tttattattt tcaactgttc ccattattct attaagatat aaattccatg gcaattcacc
     2101 cttactattt ttaagtgatg atatcatact ttctaaataa gcaggaactt tacctttttg
     2161 agaactgtta ataaattttt cagtaaattc tctaagggta ttttcatcta tctcattgga
     2221 ttcatcccat atatcatgag ttctttcggg gctataatca atttctatat ctccatattt
     2281 atcactatca tcttcctcac catcttcatc cacttccatc aaatctaatt cagtctgaat
     2341 cttttctaca taatattcaa aaggttcata tggttcaagt tttaaggaat actttaaatt
     2401 tacccactct aaggtagttg catatggtgg taaattgttt agaaatttat ttactactat
     2461 atccattgcc atattaattg ctaatgtact atactttcct tttaactcct ttgctctcat
     2521 caaatgcata gataatatat gatgaatttc atgtttaatt gtagtctcca tttgctttat
     2581 gtcaagcgtt aaaaagatta ttggattaaa atatataaca tatttagctc ccttaaaatt
     2641 cacacctgta ggactactta tatcaaatct tatttctctt gacatctgaa acaaaaaata
     2701 tccataaaaa ttatcttctt cttccataag acttaaattc actttatcta caagactaaa
     2761 aaactcatac ttaaaatctt gtggtatatc tatatcaaat ttctcattag cacgatttgc
     2821 ctttagcata gaatgagtat ttataatttc acttgcttta ttataaagct cttttgcttg
     2881 tttatcaaaa taattttcca tacattatcc ccttattaaa ctataagatt caaaatatga
     2941 ttttacaaac tcttcatttt ctatagcttt tttatatact tcaatataac tgtttctaat
     3001 atccttcata atacctatca ttaaatctac aggatacatt tttaaaaatt caataaatct
     3061 attaatatag tggtttgatt catatttatc attcttaata tttaattcta agtcttttag
     3121 aatgttcata gctgatagat aaagtcttgt atgactctca ttttttactc tttctacaat
     3181 agactcatca atagattctc ctaaaaaaac atcttcatag gatattaatg gttcagagtt
     3241 tgattcaata aaacttataa attcttctgc aataacttta cctacattac cctttacaac
     3301 atttaaaaat actgctctag gaatagaatt atttttttct ttataaactt tataaatttt
     3361 agatactctc tcataacttc taggagtggc tcttacatca tcttcattta ttttatgcag
     3421 atattctgga aaagtggaaa taaactcgat aacttttctt tcaataccct catctattgc
     3481 ccattttatc cattgtgtat ggtcactctc catatttaac caaacaaacc tattttcttg
     3541 ggctgcatcc atatctacca cttgataatc aaaatctgaa ccatatttac ttgatggatt
     3601 cattgctgct aaaatcttta catcatcatg cagtttatat ccattaattt ctctatttaa
     3661 aattaaattc ataagttctt gctgtactgt atgctcacaa cgatttatct catctataaa
     3721 taaaagaact gtcttacttt tagatatctc ttcatcaatt tctcttaatt tgttatgaac
     3781 agcatacact gttgttttct tttcagtctt atatccatta gaattaactc ctacataaga
     3841 ttctatagtt ggaagaccac ctatttcacc ctctttaagt aagtttccat caataacaat
     3901 taaactccaa tcattttctt tggcaagctt atttgctaaa gctgtttttc ctattccact
     3961 ttcacctact attaaaggta cttctccagt ggataatatt aagtcaacgc tttttaatgt
     4021 gtctataaaa ttcatttttc tctctcctac ataatcttta atttctcatc tacagctatt
     4081 ttttttgctg tcttacttcc atatttataa ataaacataa ttttatctat tggtatactt
     4141 tcgctatttc tatctataac actacaattc aaaaaatctc taatatactt ttcctcaaca
     4201 tcttctttgg ataatacttc ttttaataca ctctctaatt catctttaga tacttttttc
     4261 agaatgtact taatcactaa aatattcact tttaaaaatt ccaacatttt atttttatct
     4321 aaatcagtta taaagctaat agctgcttca ttatttttta tagctataag ttctgcatta
     4381 taactaggtg aatctatata tctcaaggca tcataactta ttctcactgc ctcctcttgt
     4441 acactttcat aaggttgttt tataaacttt atcgaatcat aattttttct tactgctaac
     4501 agttgtaatt cttcacttgg atttttagca tatttaattg cccatcctgc ttgatttata
     4561 gccatttcaa taactttatc agttgggttt ttgatataat ccagtatact ccatccctca
     4621 tttactgctt ttatcatcat atcttctgtt ggattatcta taaatttaat tgctctaata
     4681 ttattatcta ttgctagttc ttgcatctct ctggttggat tatttatata ttctagtaca
     4741 agcccatttt ctttaatagc taacaatttc atttcatcag ttggattatc tattgtagct
     4801 ataatattgg gattattttt tatcatttca ataactctca atttttccat aatgtgcctt
     4861 tctttctata attattttta tatctatatt aattataata atctattcct atttatataa
     4921 ttgtttctta ttataattaa tatacttttt aatcaaattt aaacagaata attataccaa
     4981 tatataaaaa aatttctaaa aaacaataaa attaagttat aaacaccaat atatttctat
     5041 tatttcaata cgtatacaat tagattagaa tctaacgcta gtaataaaaa attactttcc
     5101 caaagtagat ataagcctca ttttatcgtt caaataatga aaatatattt gatttcatgc
     5161 aaaaagagtg cctaatgaac taaaaagttc attttgagac actcattttt ttatataagt
     5221 ttatatagtt tttaatcgtt cgcattcaac cactcagtta agacttttat atatttctca
     5281 ttttcttcaa cgaatggcat atgtcttgaa tactcaaata attcccactt actattaggt
     5341 attctatcat acatagtctt tgcaatatat ggtgaacata aatctattgc tccactagtt
     5401 actagacaag gctcttttat ttcatgtaat ctatctgtaa attcatatcc agaaagagtt
     5461 cctgtaggag aaaattcatt ttgtccccat cctacaatat atgcttcaga acctgatttt
     5521 ttaggtcttc tcaggcactc tggagaatct tctgtcactt cacctgcaca atacatttcc
     5581 ataaatcttt ctaatgcatc attatattct ttgctagagt aatctccagt atttactgca
     5641 tcaagtaatg ctttttggtc aacctcactc atatatgata ttcttctttt ttgttctttc
     5701 tcccaaagtt ttgcagaaga aagagtagat gaaaggatgt aagacttaat accttttggt
     5761 ttatattcaa tagcatacca aatagcctgc atacctcccc aagattgacc taacagatga
     5821 atttcatcaa gtcctagatg ttttcttagt tcaataagct cctctatcca agtatctgca
     5881 ttaaatagct caggatgccc ttctacaaat gaatttccac aaccaatttg gtcatacatt
     5941 attacttgtc taccactttc agctatctta tccaatacct caaaatagtt gtgtgtagaa
     6001 cctggacctc catgaagtaa tactaatggc ttcttccctt ccgtattttc tcctactata
     6061 cggtaatacg ttttaaaacc tttaaaaggc atatatcctt cagtaatttt catgtttatc
     6121 tcctccaatt tgtaattata aacaataatc aagttcatta acaatactat ttaaaacctc
     6181 ttatacatat ttgattatgt attttaatct ttatacctac ttttataaat agtataacat
     6241 aaaagttaaa aatctctata ctaaaccaat tataaaatta tattaactat ataattttat
     6301 tttttaataa tagtatatac atatttgatt attttcattt gctatttaaa atcaatttta
     6361 agttttcttc ttataataaa cttcctcttt gtggtctcat gttagtctct aaatttaaaa
     6421 ttttacaaaa aattttcata aaataaaatt atattgattg tatctaaaca aatagggcaa
     6481 taaaataatt aagtatagtt acttaccttt caagcaatat tagttttatg ttagaatttt
     6541 attatattga tttacaggag gtttgcattt gaataatatt aattcactta aagtaataaa
     6601 attatctctt ggatgttctc ttgctatatt tattgcttgg ctattaaaat tagaatattc
     6661 aatggttgct ggagttatag tactacttac agtaaaggac actaaaagag aaactcttaa
     6721 aggctccatt ggaaagattt atggttttct tttatgtact attttttcat atttatgttt
     6781 taatatttta ggatataatt tgacttcatt ttcaatattt atatttataa ttataccact
     6841 ttgttttttg ttaaatattc aagatgttat tgctatgtgt gtagtcatag catcccatta
     6901 ctaccttcaa ggagaaactt ctatcaaatg gattttaaat gaagctggaa tatttgcagt
     6961 aggaactgga ataggtgtct taataaatat gtatattcca acaaatatac ataaaatcca
     7021 tgaagttcaa aaaaagttac aggaagaagt gagtattgta cttatagaca ttgcagatat
     7081 tattgtaaat cctaaaaaag aaaatggata tagtagagat ttatatacat taaattcact
     7141 tattgacagc agtatttctg aaacttacga taatattaat aatactttat taagtgatac
     7201 tagattcttc ttagaacata tggatattat aaaaagccaa cgggatatct tagaaaattt
     7261 gtatagttat gtatctcaac taaattctac accaccacaa gctcatattc tatctgcttt
     7321 tatacataag ataggttata ctgaatttga agctgaaaca ggcaatcttc ttcttgaaga
     7381 attaaagaga ctaatgctta gtatgaaaaa ccagcctctg cctgttgaca gaacagagtt
     7441 tgaaaataga gctattttat tcctatgctt gactgagtta aaacagtttt tagttaatcg
     7501 taaacatgct cagatactta gagataataa tttttataaa taaaaagggg agaaatctag
     7561 cagttgacct gactacaaaa tatcagatat aatattaaat gcgcccctag taagagggcg
     7621 caatgtttat aacttattct actgtcatga ttaaatcatt attcatatcg aacatacatt
     7681 atttgaccaa gtccaatatt agctttttca tcagtgattt ctattttaat agtcacatca
     7741 ttttttttac agtacttcgc taattctgca ccagaacgat ttttaccact aaaatctaaa
     7801 gaaaatggct tatcatattt aaaaggatat tcaacaggtt taccctctgg tgctaatgca
     7861 aacatcccat atggtgtgtt cataaatcct attacagcaa tcgtaagatt tgatgggtct
     7921 aaatttgatt cactacctgt aaatgtaatt gtatttactg atgaattaac tacactacat
     7981 ctaacacctt catacattac atgtttttca tttatatctt ctcctttttt tccaaaattt
     8041 ccaacagcct ttgccccttc ttgatgtggt aacatagtgt gctttccagg tggtgctggt
     8101 ttctgtgtct gttctggttt taccgtctgt actattgcac caacttttgg atatgctgtc
     8161 ttatcaaatg gtaatgggtg gctaagactc ttgtatatga tatcctttcc tgctgattta
     8221 ttagcatcaa tatctacatg atacaaacgc ccatcaccaa aacggataag attccactgg
     8281 tggtcatttg atgtgctgtg tacataataa gattcaattc ccatagcatc acacaaagct
     8341 ttcatgctcc tagccattcc tgagcatgca ccttttttag taaccaatgt ataggcagta
     8401 tttgctccct ctactgcatt ccagtcaaca tatgttgtgt tatttttaat gtgattgtag
     8461 gcaactaaaa tcttagcatc tgtggtcata ttctttgtga ttttcttgtt cataaaatct
     8521 gctgttgctt gtgctactga gtttagttgg gcttgtgaca gctttggacc atatacgcta
     8581 ttggttccag tgacatagct ttttggtgtt tttgctgtta cctctttggc tgtcatacca
     8641 taaactgtca tactcatacc tgagattaaa actattgttg taatcaataa cttggtagtt
     8701 aacttcctaa ttttcttcat acttaatatt ctcctttata aaacattgtt acttcctatt
     8761 ctctaccatt aataaaatta atttagtatt taaaaaggtg gattgtcaac atgtatattt
     8821 ctccattcat acattcgtga aatatttaaa ttttatggaa aatctaatag atattttcta
     8881 gataatttgt atacaataca actcgcaaat cagggtattt gctgttagtt tgagaaaatg
     8941 atgatgatta atacattcgg aatctttgac ctataataaa taccattgtc ttcaatcatt
     9001 aataatggta agagtaaaga gaattatcaa taatacagtt aaaatcaact ttgtatacat
     9061 ttcatacatt ctctcccaac aacattatac ttcttataat ttgtatattc aatacgtttt
     9121 ctgggaaaat ataggatata tctataaaaa tataaaaaag gagttgaact taaaatttga
     9181 ttaaaaaaaa tcaattttaa gacaactcat ttgtattata aaaatgctat atattattat
     9241 taagtaagtc aatattttag atatttatct atttcttatt gacttacgct tatagcctaa
     9301 cttatttgtt tagttgatat ataacctagt tttaagcatt attaatattt aaataatcaa
     9361 gtatcctttt tgtttcttgt agtttctgtt ctgcctcatt taagttttct tgaattatag
     9421 aaatatatat tatatctatc aacgtaagca ttgctattct tgaagaaata gctccttctc
     9481 taagtctttt ttctatattt ggtactacta gctttaaatc actaatctta ctaagatgat
     9541 tgtcactata tttagttata caaatgacct tacatccttt taattttgca ttttctatag
     9601 actttattac ttcttttgat tctccagagt aagatattgc tatagctata tcttctttat
     9661 ctacatttga agataccata agttgtgtat gactatctaa agaggtaaat gcctgtttat
     9721 ttatccttaa aagtttcatc tgtaaatcta gtgcaactaa agccgaacca cctactccaa
     9781 atatatatac atttttagcc tttattatct gttcaactgc ttcttttatt atatcttttt
     9841 cattcaacaa ccttgtttgg ttgatagtct ctatgttttt gtttgtaatt ttgtttataa
     9901 cactatctat attacaatca tagttttcat ctacatatgt gtattcattt tctttgccca
     9961 tcaaatcttt agctatctca atttttaaat ccccaaaacc cgaatatccc atttttctag
    10021 aaaacctaac tatacttgct gtacttgttt ttgagtttga cgccaattct gatacagaaa
    10081 gcttacttat agcctctgta ttattaagta tgtaatcagc tattttttta tctacttccg
    10141 ttaaatcttc atatatctgt tttaatttta aaatgcaact catactctat cacctctacc
    10201 tatttactat aatatttctt cttagcaagt aaacatgctc ctaaatctgg agtcaacact
    10261 ggtctcacta taccaaatct cgttttattc aaatattttt taagtggctc taatattaaa
    10321 tctcctgact taaacactcc acctgaataa gataccttta tctttgtacc ttgttcaaaa
    10381 tgatgctcta atcctattat aagcctactt aattcataag ctgcatcatc aaatatttta
    10441 ctagcagtat tatcaccttc aataactgct tttaaacata tcgtagcaaa ctttgctatt
    10501 tctattctat ctccttgtat ttcatcattt acatatttta ttatgtcata atcattctct
    10561 atattaagat gtctttttat taaatcatat aattttgttt ttgaacacct tccatcagac
    10621 tgcattgtga acatttttaa ggtagccatt cctatataat acgctgaacc ttcatctcct
    10681 aatacatagc cccatcctcc acatctgtga agattcccat ctttatctaa agcctgtgct
    10741 attgaaccag tacctgcaat tatatttata cctttttccc catttaaaga acctgctaat
    10801 gctatttcta catcattacc tagagtataa tttatatgac tatatgcatt ttttgttgct
    10861 acttctaatg catacaatac ttcttttatt tttccatatc cagcaagtcc aacaaatgca
    10921 tacgttattt cttctacatt tatcttagca tctttacaaa cttcttccaa cccagttatt
    10981 aataatttcg ttagattatc aaatcctatt tgattataat gacaagttcc tttggttatg
    11041 gttcctacta tatttaattc ttcatctact aatgtaaatg ttgttttcgt tcctccacca
    11101 tctacaccta aaaaatacat actatcctcc tacaatatct ttaataattc tttcaatact
    11161 taatatctaa aaataacctt ccaatgctgt ttttttaata ttgaaaggtt atcagtacta
    11221 ttatttattt tacaattttt ctattgattt taaagcttca cttatataac cttcttgtct
    11281 atctaggata ctctttgcac tatccttatc taaaccactt tttatcataa atatagccaa
    11341 tttaacgcta ttatctgctt cttctaagaa tttttctgca acatcatatt ttacatttgt
    11401 agctatcatt attattcttt ttgctctttc aaccaacttt tcatttgatg tcttaacatc
    11461 taccatcaag tttgagtata cttttcccat cttaaccata gaagctgttg ttaacatatt
    11521 acataccatt ttttgagctg taccactttt cattctagtt gaacccatta ttacttctgc
    11581 ccctactata atgtttattg gtaaatccgc ttttttactc atttcactat tcggattcat
    11641 agttattgat agtactgcac accccaattc cttagcatac tccattgcac ctataacata
    11701 aggagtcctt cctgatgcag ctataccaca tataacatcc ttagatgtaa ctcctctatc
    11761 tattatatct tgctttccaa gttctttatc atcttcagca ccttctattg ctttaaatat
    11821 tgcttgattt ccaccagcta ttatagcttg aacaagctca aaatccactc cgtaagttgg
    11881 aggacattct gtggcatcta gtattcctat cctaccactg gtgcctgctc ctatatagaa
    11941 taatctacct ccctgcttca ttctatctac tattatatta acagcttttg ctatactctc
    12001 cttttgttct tcaacagcat aggctacagt tttatcttcg ttatttatta attctattat
    12061 gcctaatgta tcttctttat ctatttgcaa tgtattctta tttctacctt ctgtaactaa
    12121 attttctaaa gtttttatta acatatcatt tcctcatttt cttaatatta aatttttaaa
    12181 cacattttaa ttccaacttt cataattata tttttataat aaattaatat tttcataata
    12241 aattattttg ttgagggttt aaaattaaac ttttgccatg gtttaatata atctaatata
    12301 aataattctt cttttcttat atatcctact acattcgatt taccactgtt tttcatatca
    12361 gtcaaggcta tttgtaactc ccctgcataa tgaccatatt cactactttc aattactata
    12421 tctcctcttt ttattatttt aggagtatta aatagttcaa atttgtatcc tttatattta
    12481 acacgagatt gagtagacct tatcagactg tcagatatgt cccctctatt aaaatgtatt
    12541 tcttcaaaga gtatcttctt ctcaacttct cgtatatttt cctctattgc tacatcaaat
    12601 gtaaccatat cttttctcat agaacctaat acctttagct cctcatatgt tggataacaa
    12661 tttgatatta ttatatcatt tatattattc atagctatta tatgctttac ttgaatatct
    12721 ataggtagat ttctgtgcat ctcaattgtt ggaagcccat cagttacagg ccatggtcca
    12781 aatgtagttt cattttgact tgttacaaaa actgctgttc ttaatccata cttattaaat
    12841 ctttctgtac attttataaa gtgttctaaa tttaatccac tatatctatg tggataaaag
    12901 ttatgacatc ctgtcaagtt ttctttattt ggttggtagt ccattattgt atctatataa
    12961 ttagtattat tactcatatt gatttctatt ttaagatttt gaggattaaa agtcattaat
    13021 gcctcttcag aacctgtaaa tcctatatca agccttattc cgtctgcctt cgtcttttta
    13081 aaaaaagtca aatctttgta gcttatttct aattcaccaa atactctggg atttacatca
    13141 agtattacct caaaaccttt aaacttagcg tattcattta tacgtttaaa ttttttaatt
    13201 atattttctt tagtatcttt aactgataat aaacatgaaa atatacgtgt aaatccattt
    13261 tcagcagctt tatctatata atcatatatt tccttagatg ttgttttttc tggataaatt
    13321 gatattccta atcttcccat ctgtgtcatc tccttatatc ctatatgtgt ttaaatttat
    13381 ttcgaattac acgaaacttt tttaaaaatt tggtgaaaaa aagtttcata tatatttata
    13441 aaaaaagaaa agtttacctt ttcttttttt ataaacaatc aacaaaatat cttcgttact
    13501 tatttcataa ctgttatact ttgaaattta tactaatcta atatagtttt taatttccaa
    13561 tatagtttct aattaataag cttcttgctc ttcaactttt tcttcatgaa gcaatctctt
    13621 atcgtacatc ttgacaaatg gatagtaaat tattattgat atagctatac ataataagtt
    13681 aagaactgaa gctctccaat ctccaccagt ggctaaatat gcccctactg gtcctggtaa
    13741 tgtccaagga gcagtaacta ctactctgga tactaaatta gcactcatag caaaccatga
    13801 aactgttgcc attactattg gtgctacaac aaatggtatt attaatattg ggtttaatac
    13861 tattggtgct ccaaatatta ttggttcatt tatattgaat attgctggta ttacagttgc
    13921 ttttcctaaa gactttccat aagctgattt tgctctaaat gcaagaagta aagctaagcc
    13981 tattgtagct ccagaacctc caatccatat aaaccattga aaaaagggtt cagcagcaat
    14041 acctggaact gcttgacccg cagcatatga agctgtatta gcatctaata attgtatcca
    14101 taaaggtctt gccaatgaac ctattattga tacaccatgt attccaaatg accaaaaaaa
    14161 cgttattaaa aatattagta taattactga tggtaatgtg tcagtggctg aaactaatgg
    14221 agttactaat ttagctatga agccatgcca atcaaaccct aggaaataag taacaatagc
    14281 cattaaaatt attattaatg cggctggtgt cagagcttca aaagaccttg tgactgatgc
    14341 tggaactgct tctggcatgg ttatcttgaa tttagatttg tcagtaaatc tataaatctc
    14401 aacacctatt atagatgtta caatagcaac aaacattgaa cctccaccaa gattagccat
    14461 tggcatttgc catccttgag gaattgaatt ataccaagct aaaagttctg gactcttttc
    14521 aactatcttt aataactcag gggtaagacc ctctattgca gtaggaacta ttgtcaaaag
    14581 gaatgtagct gtagctaata aacctcctgt caatccatct aacttatatg actttgccaa
    14641 actataacct atgccaaatg tagaatatac tgatataata aaaaatgtca ttctatatgg
    14701 tagtaatatt gtaactgcat tttcctttag atactgagtt accccccatg aatctggtaa
    14761 tggaggaaat gctaatatca agaaaaatga cccaactatt ataagtggta atgaagctac
    14821 gattccatct cttacagctc ttaagtgacg ttgttctgcc aatctggcca tagaagttga
    14881 taatttagtg tctaaaaatt ctgtaaattt atctagcatc ttttctctcc ttaattaata
    14941 tttattttta ttatcattaa aatttttata aaatcaactt tcttctaatc taacaacact
    15001 gcaactacta tttactcaac agttcttgaa cttgttttaa taactttggt cctcctaatg
    15061 gactataagc ttgtgcttgt attaatgcac atggtacact aacctcatca gcaaaagctt
    15121 ttaaatagtc aaatctatgt ctgacttgtg gagctaccat agcaatatcc catccatttt
    15181 taacttcatt ctcaaactct tgagtaccaa cagccaaaac atctatatct attccatttt
    15241 ttgctccttc tttttgtaat gcattaactg ctatagcact tgacatccct gatgaacaaa
    15301 caaataatac tctcatattg attccccctg tttttttatt tcttttcaat cattatagca
    15361 tgtatttttg attacaggaa tacgtttgta tatatttttt catattttac attttaaatt
    15421 gtaattttat ttcatgcatt tatatcttgc tttaattgct ttatttatct aatcattctt
    15481 ttgtctaaaa ttacacacta cttaaaatac tcatatcttt tcttaaaaaa gaaaaaagac
    15541 tatcttatta ttaacataaa atagtctcaa tactttttta aatatgtatt cctttatata
    15601 tccagaagta acctattatt gcaaatgata aagcaaataa aatatacagt ctaaaaaatc
    15661 ccaaagaaaa acctctctta gtttcaacaa agtatcttgt atatgcaact gcaaaagatg
    15721 taattaatga atttcctata acaactccaa atctattatc tattccaaat atagataata
    15781 acaaaggaaa aactacagta cttacaagag atgaaactat tagtattaat ccacttttaa
    15841 aacttatagt tggaatatta taatctgttc tttgattttt ctccatttgt ttcttagtat
    15901 ttttattact ttttgtactt tttgcatttt tataattatt atttttcttt cttttctttt
    15961 ttttattatt agacatgtca tttacctcca aaaggtttag gtgttatatt ccctaaaata
    16021 actttatttt tagcccctgt tgcagatatt acattagaaa aagaattatt taatgtttca
    16081 ttgcccaaga ttacaaatgc caaagcctcc tttgcatcag aagaataccc atattcatct
    16141 tgagttaata cttcaacctc taataataac tcttttatat atcctattaa agttagatta
    16201 tgagctcctc cacctccaat taataaagta tctacattaa cctttggaag tatgaagttt
    16261 ttataattat aatatatagc ttttgccgta aacatagtta ctgttgctat caaatcatat
    16321 ttatctacat gtttatattt gttaagcatg ttatccaaat aaacttgtcc aaatacctct
    16381 cttccagtgc ttttaggtgg cgcttgacta agatacttat gactcatcaa atcttttaac
    16441 atttcttcat taattttacc ttttgatgca aagtatccat ttttatcata ctttctatta
    16501 aataatcttt gacatactcc atctattatc atatttcctg gtcctgtatc aaatgcaaat
    16561 acatcatcta tgttacatga tttaggtatt atagtaatat ttcctattcc accaatattt
    16621 tgaagtgcta aatttttatt gttatcacta tataataaaa attcactata tggtactaat
    16681 ggagcacctt ctccacctgc tgctatatcc attactctaa aattagatat tacctttgta
    16741 tttgtttcat atgctataac tgctggttca cctatttgaa gcgttgaaga tacaaaatta
    16801 ttataacttc ttggtatatg aaagattgtt tgaccatgag atgcgataaa atccaaattt
    16861 gctatatgaa agtttgcttt atgacacact gattttacag ctttactaaa taaatatcca
    16921 agcttaaaat ttaagctaca gattaaatca acactcgatt cctcttctat acagcacttt
    16981 cttatttcgt cttttatatc tttaggaatt tctagagtta taaattctat ttgcttaact
    17041 ttagtatttc taccattccc actaatttca gctagtactg catcaactcc atctaaggaa
    17101 gttcctgaca ttaaacctac actatacata gttatcatcc ttttaagttt atagttattc
    17161 ctccccaaat caaggtatta agtttaataa ttttagaatt aatcttattt catctttaat
    17221 tacgataatt tactcttacg aaattaatcg agttaatctt atacacgttt tttaccttag
    17281 ctaaatctta cattaattta ttttcagtgt caatgtaata ccatgtaatt ttttttcaca
    17341 aacaaaacat attctctctc caaaaaccat attaaaatag ttactacaaa taaaaaacat
    17401 accaggtacc tatctgatat gtttttcata tataataatt attattttat aatatagatg
    17461 gagcatcatt taaggcacta attaaatctt cactattttc aagaactaaa tttattctta
    17521 ttgtatatgt cgatttgtat atgatgatag attttatagt ctaataacct aactatctat
    17581 gcttaaataa tccgatatat cataaaagta gatattaaaa attgtatatg aaccaatttt
    17641 cgaacatacc tcaatctcat gccctaaact atctatcatt ttctttacaa aatacatccc
    17701 cattccagta gacttataca attgatttct tccatttttc cccgtaaatc ccttgtcaaa
    17761 aaccctagat atatcttcct catttatacc tataccaaaa tcttttatag ataataccac
    17821 tccattttct aacttttttg aattaaattc aatcttacca acttcctttg aatactttat
    17881 agcattatta ataatttggt ctaaaacata tgacatgcat ttagaatctg tgtaaacatc
    17941 attttctaat tcattcaaac taacttctac attatttttt ataagcaaaa atgaattatt
    18001 ttttatagac atttttacca atttttctaa attaaattta tttataaata catcttcaaa
    18061 catagtgttg cttctactac tatacaaaat agagtttact aaaaaattta tctttgcaac
    18121 ctgattctta atatccaaac tatcttctat gctacttaac ctatctgtaa ttatacttaa
    18181 agaagatatt ggaattttta tttcatgaac ccatttagct atgtactcat ccaattcttt
    18241 taatgattgt ttataagtct ctatgtcact attatttttt atttttaagt tttctataat
    18301 atcttttatt agattttctt caaaagattg tccatcaact aaataactat ctatttcacc
    18361 actattttct atacaattat ataaatttgt gtaactattt ctaaaattta tataatctat
    18421 aacaaaaaat accattatta taaacagcac taaaaaatct aaatatatta actctgaata
    18481 tttattttta aaaatatcta tagcaaataa gtagatattt attatactaa ctacaactaa
    18541 aggtagagca atatacttag acctaccttt caaatatttt actagcattg tattatatat
    18601 cctatcccct tcttagtttt tataaaatca tcaaaaccca gttctttaac tttacttctc
    18661 aatctattca tatttacagt aagtgcattg tctgtcacaa actcgtcatt atcccataga
    18721 gtcatcataa gactttccct tgatacaacc ctattcttat tttttaataa taatgttaaa
    18781 atttttattt cgtttttagt caactctata cttttatcct tatacttatg ttttataatc
    18841 cctttcccca catcaaacat caaatcttca caatacaaaa tactattaga atcactataa
    18901 ttatagactc ttcttattat accatttatt ttggtaacca atatttctat agaaaatggt
    18961 ttggtaatgt aatcatctgc tccattattt atgcccatta ttaaatccat atcagaatct
    19021 cttgatgata aaaatattaa tggtacattt gaaatagctc ttattttttc gcaccagtaa
    19081 aatccattat ataatggtaa atttacatct aaaagtacta catctggttt tttatctata
    19141 aattcatcta atactacctc aaaatcatca attaattcta ctttaaatcc ccatttttca
    19201 agtgctaacg caatctcttt tgataaatca atatcgtcct ctactaataa aattctatac
    19261 atttgtatat tctccattat cctaatattt cactatgcta tgaattatta atatcataac
    19321 atattttata tgaatttatt acccaaatta tttagttaat caacaaggag actcttatta
    19381 caagaatctc ctttctcttt attaatatat caactaattt ttatacacta cactcttaaa
    19441 cttattaaca gatactgctc catatattat gaatattatc aaacatatca ctaaacttag
    19501 tatatatata ccaaataaat cggtcatcaa taaatctcct gctaatctca atgcaaaata
    19561 actagatata gttgcactta acattggcaa agtataaaaa atagctactt ctttaagtat
    19621 agctctattt atatcttttt ctgtagtacc tatttttaac aaaatcttat atttctcttt
    19681 atcttcatat gcatcactat atattttcat atatattata cttccagcta caattgcaaa
    19741 gactaagaat aaaaacacaa gcactacata tgcaaacttc atccattgac tctcaccttg
    19801 agctttatat ccattttgaa tatacccatt ttttaaactt aaattctttt caatattgtt
    19861 gaacattact tgactatttt cttcttgaaa tacctttgca ccataaaaat acacttgttg
    19921 acccttatct cttagtttaa aatatgtatc atcatttacc actataatac tttgattaag
    19981 tcctgtccct agtactggaa cttttacttc tccccttgaa atattatata actgaccatc
    20041 aagttttatc ttggcttctt ttgtagctat tgatgctatc gtttgagggc tttttaactc
    20101 tattacatta tttccactaa ctaatctttc accatactct tttaattctt tatcattacc
    20161 attcacttta agagtattac aaaaatcact atatttcatt atagtatctg ttgtatcata
    20221 agcttctttt atatctgttt tctttaaaat tgttagttct ggatttactg agtactttat
    20281 cttattagta tcttttatta tattctctat ttgtttttta ttcacttcat ctttatccat
    20341 tgaaactatg ctaatagtgt acacatttct ttgttcttgg gcattcttat gtatatgctt
    20401 catagcaact gaagcaccaa gagttgatat tgttgtagtt ataattatag ctattatagc
    20461 ataagtttta tagtttttat ttaatcggta gtttatattg tttattgcaa ttatattatt
    20521 tccattaaat aatatttttt tatttttgat tataatatca aatataacag gcataactga
    20581 tttgaataat ccataagtac ctactattat tatacctata acaggtagta cactagttat
    20641 atctccagtt cttatattgg tagaaagata gtagccatat cctattacta taattgatac
    20701 tatagctaat atatatatac ctattcctac ctttggtatt ttttcttgtt ttttagatat
    20761 attgattaga tttattacac tacttctaca tattgttata aaacctttta cagccattat
    20821 tataaatata gaaataaaga ttaaagtagc atatagtata gcttctatac taagtccatt
    20881 agataccttt atattaaatc cagataactt tattatcact acttgaaaaa atctagatgt
    20941 tattatccct ataattattc ctgttataca agaacttatt cctataagca tattttctac
    21001 aaaatatatt tttcctatag tatataaatc taatcccata aaagacaata ttccaatttc
    21061 tttagaccta ttcttaaaaa atatatttgt tgcataccat ataaagaaaa ataaaaatac
    21121 tgatagtatc attattacag ctactaacac tgatagtgta aattttttat tttcctcttg
    21181 taacacattt atagcatcac catatcttaa taattcaaaa ttagttagca caactactga
    21241 aaaaaccata gatataaaaa atgctagata agttcttatg ttactcttaa aattattata
    21301 agcaattcca agtatactca cttacatctc acctcccata gaggctagca tatccataat
    21361 ttttccataa aattcttttc tactatttcc tttgtttaat ttacacttta tatttccatc
    21421 atttatcata tatacttcat ttgaataact tgctgttaga gggtcatgtg taatcattat
    21481 aatagtagcg ttattttctc tgtttatttt ttctaatgat tctaaaagtt catatgctga
    21541 tttagagtct aaagcaccag ttggctcatc agcaaatatg actgttggat ttgtaattaa
    21601 tgacctagct gctgcgcctc tttgtttttg tcctcctgat aactgatatg gatatttatc
    21661 taaatgattt tctaatccaa attttttagc tatagaaaat accttagctt ctatctcctt
    21721 acttttcttt tttcctaatg ctagaggaag tgctatattg tccattagtt tcatattgtc
    21781 taatagatta tagtcttgaa atataaaccc tattttttct cttcttaatt ttgatagttc
    21841 attatttttt acttttgata tattaattcc atctagtatt atttctcctg aagtttgttt
    21901 gtcaagtgta gacattaaat ttagcaatgt tgtctttcct gcaccagatg gacccattat
    21961 tgatataaaa tcaccttcat tgactgtaag agatatattt tttaatacat ttgttttaaa
    22021 tccttttatt ccataatctt ttgatatatt ttttattttt aatatttctt tcataacaat
    22081 agcctccagt tgctaatttt ttaatatata tttattttaa aggttttata taaataaatc
    22141 cttaagtaaa tattacattt taactgttag cattacactt ttgtaacata tggaagacta
    22201 ttataattat agtagaaata tcttaaaata aaagcatgat ttacttcttt gtataaacaa
    22261 agaaaagaag ctcctaagaa gcctctaatc tatacaatta ttcttctcta taaaaaacca
    22321 aaatacacat tgattgtggc aaatacagat actataaaca agtgatatta ctactatata
    22381 ttaaaacaat tattcctgaa gataattcaa taagactatc aagcaataag ctaagcatcc
    22441 aaaaagactt aataccatta atgtataatt atacccaata ttaatgctta aaaaaccagc
    22501 aacagttgga ataatcagag atgaacctcc cataaatata cttaaagcta ttaattggct
    22561 actacttcta agttttttag gagaaacttc aaatattaat ctttttgaag taattaaaat
    22621 taaaggagta gaaaacactt gaaaaacaca cagtataata atgatatttg aatcatttgt
    22681 taaagcaaat aatagaaact gaactgttat cataatagca cttaatttta ataagtttaa
    22741 actgctatat ttttttaata aataagaacc agccagatat gttggtatct ctaacaatga
    22801 ctgtaaagac cactttaaag atatctgaga atttgtagca tttaaagcta tcattttatc
    22861 tattacagta cagttatttg ctaccaccat tgagtataat aagaataaaa tacatactag
    22921 taagatatat tttttatctt ttaataattc aataatatct ttattagtta catttatttt
    22981 cttattaatc ttttcaatat caggtaataa tagtatattt ataataagta atactacaat
    23041 taaaccaatc gctacagata atccaaaata tgagaatcta agaatcaagt aagatgctac
    23101 catacttcca atagcccatc caatcgaccc aaaagtctta ataaatgaca aataattatt
    23161 aatattatct ccacaaccta ttatccatgt atcatataaa ctacagcaag tatttaataa
    23221 tcctcctgat aaagcaacaa gtaataagta tattataaaa gttgcagatt cagtagaaaa
    23281 cataataact gatgcaattc cataaactga taaactaatt aaaacaattt ttttcattgt
    23341 ttgatactta tcagctaata ctccaaataa taattgaaat aatatattgg ttattgcata
    23401 actcgataat ataattcctc tttcaaacgc tgagtaacct aattttgata agaaaggtgt
    23461 gaattttgtt gaagcaaagg caaaaataat atatgagaaa aaatagaata tgataaattt
    23521 aaatttaatc gctttattaa tcataatcaa tcaaaattga taactttaat accactattt
    23581 ccttcctgat ttaagtttga aagatgatat tttaccgtat taggattctc tacaataact
    23641 aaaggaatat ataaagtatt ttcaagtaac ttattatcaa tttctactaa cttttccccc
    23701 atattaactt cttgaccttg tttaacttct aatttaaagt tttctttttt tgttgttgca
    23761 ctgtccaatc caatatgaat aagaatttga acatcatttt gcaatttaat tcctatagca
    23821 tggttagttt gagcaataaa acttattact cctttagtcg gtgatttaag aatattgtcc
    23881 tttgctatca tagcaaatcc atctcccatt accttagttg aaaatacttt atcaggaatt
    23941 tctccaagat taattatttt tccatcaatg tatgaattaa ttgtaattgc ttcttttttt
    24001 cttttaaaga acattataga cactcctcta cataattaac tttattctaa ttatcattac
    24061 aaataacgat attaagcata ctgcaattga ggaaaaaaga gccttatagc tctttcccca
    24121 ttgtagttct ttaatactgc ttatgatttg tttaattcca acaaatttat aaatataggt
    24181 aaacatgata acaatagtta ttgtgtaaat ttggaatatc tgccctaata agactaaata
    24241 gaaagcaatc atgttttctt ttatctcttt attctttttt atattgtaaa taacatatgc
    24301 actgtatact gcactaaagt tagagattaa atttaaactt gcattaacat cattgtttaa
    24361 aagttgtgaa aatgaaatat ttgtaaataa tgatgtcaat tctaaataca ctatatataa
    24421 aacaggtaat attagaaaac aaaccataac cactgaaaga aatatatttt catcttttaa
    24481 tactgctgtg atatttttta ttttatttgt tatcatgttt ttgttctcct tatttaaaag
    24541 ttggccagaa atccttatta gccacaatca tttcatctaa gatttctttt gctacttttg
    24601 cacttggaac agttttactc atagtaaacg actgccataa tttttgataa cttttttcta
    24661 tccatgcttc tacagctaat ttttcagaag caacttgttg ttccattaaa cctttttgga
    24721 aagttggaat tttacctata gacataggtt caaccatatc ttttccaaca aggcaaggaa
    24781 tttcaaccat tgcatcatct gcaaaatttt caatagctcc attattagga acaatcagta
    24841 aaaatctttc ctgagtatta aatgctaatg cacaagctaa ttcaacaata aattgagaat
    24901 gctcacttgc atcaaaccat gtatcccttg ctgtgccatt ttttataatt ctttcacatt
    24961 cttcgaatgt atccttttct cttctatcca taatttcatt tgctcttgta taatttggat
    25021 tagcatgttc aacagtttca tcagccatta aataatattt taaataagta ttaggtaatg
    25081 tagttggttc tatttctaat aagtcctttg ctgctctcat agtttctaac caactagatt
    25141 ctttgtgttg accttgaggt gtaaattcct catatccttt tttagcacaa tattcttgta
    25201 atttaggcat taaatcattt ccttctttgt ccttaatact tgtccaccac ccgaaatgat
    25261 tcaatccata atatctaaca tcaaagtctt tacgagaatt taaacctaaa actctagcaa
    25321 atatttcctc taaacatact ggcatatcac aaatattaat aattcttgaa tttggtctta
    25381 atacacgaca tgcttctgca actatagctg ctggatttga ataattaagc atccaagcat
    25441 ttggagaata tttttccata taatcaatca tttcgattat tggacctatt gttcttaatc
    25501 cataggcaat tccacctgcc ccacaagttt cttgaccaac aaccccatat tttagtggaa
    25561 ttttttcatc ttgttcacgt aaagcatata aaccaacacg aatttgtgcc attacaaaat
    25621 caatatcagt aaatgcttct tctggatttg ttgtataact aaatttaatt tctggatttt
    25681 tctctctaac aattattgca catgcttcag caacttttct ttgtctctca tcattattat
    25741 catataattt aattgattca ataggaaatt tagcaacact ttcaattaac attgttacaa
    25801 ttccagcagt ataagtacta cccccaccag caattagtat cctctgtctt tttctttcca
    25861 tctttccttc tcctttataa taaattttca aattcttctc ttacacttgg tactgaaagc
    25921 ccaataataa tctgaatagc ttttccatta tgcactaaac catgtgcacc tactgcctta
    25981 aaatcttctt ctttagaaat aattgagtca tctttcaagg ttaatctcaa cctcgtagca
    26041 cagtttgtaa catctacaac attttctgct ccaccaacta attttatgta ttcagatgct
    26101 tgttgagagg ctttactcaa tttatttcct ttttcatctt tagtttctaa gtattcattt
    26161 ttagtatata gttttgtttc ttcactatct tcttctctac caggagtttt catatcaaac
    26221 ttcttaatta agaaagtaaa tacaaagaac cagataaatg aaaatataat ccctacaaca
    26281 acttgaacaa catatacctt ccagtgatac attccaagag gaagccaatt taatacaacc
    26341 cagttaataa ttcctccacc aaaatcacca acaactccta aggcaaacga aatcgtttgc
    26401 aagcacgcat ctaaaaatgc atgtactagg aataaaagtg gtgctacaaa taaaaatgta
    26461 aattctaacg gctcagtaat tccagttaat actgcagtaa gagtagcagg aatcattaaa
    26521 cctaatactt ttttcttttt ttctggcttt gcagttacat agaaagcacc aaatacacct
    26581 aatgaaccaa acaccttcga taatccggat aatgaaaatc cgattggata catttccttt
    26641 agagtcttag ctgaagtttg aaaatcctga atatgagtag cccaatatac tgatgtacca
    26701 cctggaacaa caacactatc ataacaaatt ggtgcatata caaaatgatg taatccagtt
    26761 ggtattaaaa tcttttgtaa gaatgcataa caccaaactc ctatatttcc actatttata
    26821 aaaaacgttt gcaaatgtct aataacattt tgaatatgag gccaaataaa acaagttaat
    26881 aatgcaactg gtatcataac aaagaaacaa attgcaacta caagtgatga gcctctaaat
    26941 aatccaataa aatctggtag ttctttatca taaaatctat tatgaatata aatgacaata
    27001 cctgatatta acaaagcacc aaccatacca gtatctaatg ttttaatacc agcaatgctt
    27061 acaagtccac ttccagcact tatttcagct gcgaaatcaa ctccaaagct ttttccccaa
    27121 aaacttaaca tagaacctac aaaataatta aaagtcaaat acgttgctaa agcctccata
    27181 caagctcttg cttgttgctt ttttgcaagt gctattggta aagaaatagc aaataataat
    27241 ggtaattgat taaataccat ccaagctcca ctagatatta caccccaaaa ttggtaccaa
    27301 atattttctg gtgtagcaag tgaacccata attgactgat tttgaaatac aattgagaat
    27361 ccaaccatta tcccagcaaa agcaaataat agtgttggtg taaacattgc ccctccaaaa
    27421 cgctgtattt tcttcatcat ataaattact cccctcctca tatttttagt ttatctttgg
    27481 ccttagatat ctattatttt aacaggttaa taaatatata caatctttag ggtacatttg
    27541 gagcttattc acagtcaaat aagtttttgc acatttttat gttcaaattc tcataaaaaa
    27601 aaagaatgac tacaatttgc aatcaatccc ttctgtaatc attctatgcc ttttataaat
    27661 actatattta ataaataata actcaatcaa tataaaatat aacattgtca atttataact
    27721 agggtgcatt tgtaaaaatg agatattagt cgctgaaata taaattgatt catcacttaa
    27781 atcagtcaat gtattatttt taaactctgt aattgaaatt attttagttc ctattaattt
    27841 taatcttcta gcaatattaa gtgtattctc attctcacca cttagggata taataatcat
    27901 aacatcattt ttactgacaa tattgtaaaa agaatcatca aatgttcttc cctcaaaatc
    27961 atatattatt tcttgacatg ataagaacat tctctttatc tgttttgcaa ctacattttg
    28021 aatatcacct gaaccataag cgtatattgt tcttgattca taaagaagtt tacacaatgc
    28081 atcataatct ttattttttt gatcttcaac atacttaatt attgaattac atgcaatatc
    28141 aaatgactga ttttctatga catgaacctt ttctgattcc cagcttaata atactttaaa
    28201 ctcactgaaa ccttttaaat ctaatttttt aacaaatctt gtaatagttg cacgagacac
    28261 attacatttc atagcaagtt catttattga catcttacta caatgttttt tatttgcagt
    28321 tatatatttc catattaata aatctgtatc attcaattta ttataattta aaaaaatcat
    28381 ttcttctaat ctcataaact attaccccct ttaattaaaa atcgtcataa atattatacc
    28441 aatattcaac attaaattac agcgtattct tatatttact atttagccca taaaattgac
    28501 tataaaaaat actgctataa ttgatgttta aaaaaatgta gttttaattt tattctttta
    28561 tctaataatt aatattttca agttctagtg cttttcttat acaattatca tctttaaaat
    28621 cagcagtcct tttacaatct tttactttaa attctggaat agttttaaat tcctcatcac
    28681 atattccatt cttatttaaa tacatacaac ttgccattct aactattaga ccactatttt
    28741 ttaaattaaa caatacaggg tctatacacc ctccatctcc gccagtagtt tcaccaacta
    28801 aagtcgcaaa tttagtttct ttacaaaaca ttgcaaatga ctctgatgat gaatatattt
    28861 ttctatcaac aagtaagtat atattaccct taaattttga atcatctgta gattttatag
    28921 tataggaagt atcttcaaaa tcattaaaat cttttataat ttctttaggt gcattttcct
    28981 ttacacttgt tggtaaattt tctacaggat ttaattttat atttcttgca tttgtatatt
    29041 gttttactat ttcattatta tttctataaa ttctataacc atttattttg acatcatttt
    29101 ttatgagttt agatacgatt ccctgccaat aactatcact tccacctaaa ttacctctta
    29161 tatcaataat taaagcttta tgtttttcta aagtgtcaat ataatctcca attattttta
    29221 aatcattatt tatagagcca ttctttgatg acattgatgg taaatacata tatccaatct
    29281 gacccttaat aacatccttt aaaattaatt ctttttttac aaaaatatca tctggcattt
    29341 ttattttaag attcatagag tcatatctat ctacaacttt tttatcatta agaaaatcat
    29401 accaatcatt attagagtat gatttcttaa aaagttcata tcttttctta ttatctatta
    29461 cttctgtgtg tttattattt aaatcagata atatagatga catttcttct ataaattctt
    29521 catcattttc agtgtttttt attcgttttg tatattcatc cttatttgct aaccaatcaa
    29581 tattattaag cctcttattt acacctagat atggatatcc tgtctttaca acattataca
    29641 cataattaaa gtcatccacc ttttcctctt cagacaactg attcttttga gaataagtag
    29701 aattacctaa taagattata aatgctaaaa taattactaa aaataatact ttctttttca
    29761 tgtaattctc atccactttc agattttata ttttaataca ttgtatcatt attttattat
    29821 cacctaaaca gttaaaagaa attcttgaag atatattcca ctattattct ctttaaaaat
    29881 ttttatttgt attttcaaaa ttatttcatg aaagttataa aataacaatt atattcaaat
    29941 atctatttta ctgctatatc ctgaaggtaa attcttatca gtgatatact aaaaaagata
    30001 tgctaaaaaa tttatccatt ataatgactt aaatttagat actttactga tttttattta
    30061 ttagttgaga aagatattga taattaaaaa tattgataac tatagaaatg attatttttt
    30121 atagaaattt taaggatata aactaattat acttaagtct atatccttaa tttatcatga
    30181 ttatgagaat ttttacaaat acttatcttt gtccgtttta gaataaaaac aagaacatat
    30241 cctgttattt tattcaaaca tccaaattta attactacac aattacttat tagaaattgt
    30301 atctacttca tatatatatc tgttaagtat agcttctagc atttcttgtc ttccagactt
    30361 attctttata actggcattt gcagtgcata agattctaac tctttaaatc caaccttacc
    30421 ttctactata tccttaccta taccttcact aaaacttgca tatctttctt tagtaaagtt
    30481 ttcaaatacc tcatcttcta ataatttatg agctattaat aatccctttg caaatgtatc
    30541 cattcctgca atataagcta ggaataagtc ttctacctca aaagatgctc ttcttacttt
    30601 agagtcaaag ttaaatcctc ctggtgctat tccaccttgc tttaacactt catacatagc
    30661 aagtgttgca tcatatatat ttgttggaaa ttggtctgta tcccaaccta ataataggtc
    30721 tccttgattt gcatctatac tacctaaaac attgtttatt cttgctatat ttaattcatg
    30781 ttggaaagta tgtcctgcaa gtgttgcatg gtttgcttct atattcattt tgaagtattt
    30841 atccagatta tactttctta aaaatcctaa aacagtagca gtatcaaaat cgtattgatg
    30901 tttagtaggc tcttttggct taggctctat taagaattgg ccagtgaatc ctatttcctt
    30961 agcataatct acagccattt gtaagaatct tgcaaagtta tccatttcaa gttctgtatt
    31021 agtgttaagt aaagtttcat aaccttctct tccaccccag aatacaaaat tttctcctcc
    31081 taagtactta gttatttcca tagctttttt aacctgagct gctgcatatg catatacatc
    31141 tgcattacat gttgtagatg caccatgaac aaatcttgga tttccaaaca tattggcagt
    31201 tccccataat aactttatac ctgtcttttc catattaact tttatatact caactatttc
    31261 atctaatatc ttatttgtct cttctaaatc tcttccttct ggtgctatat ctctgtcatg
    31321 aaaacagaaa tactcaattc ccatcttaga cataaattca aatcctgcat caactttaat
    31381 ctttgctatc tcaattgggt ctgttatatt attccaaggt ctctctactg tgcctactcc
    31441 aaatgggtca ttaccctctc cacataaagt atgccaatag ctcattgcaa atcttaagtg
    31501 ttctttcata gtcttattac ctaccacttg ttcaggattg tagtatctaa atgctaattc
    31561 attgtcagat tttactcctt caaattttat ctgtcctatt cctttaaata tttcactcat
    31621 ttcatgtcct cctaatattt tacacatata atatatgtag tatgttttct taatttaaaa
    31681 ctattttcta tctttatttt tctttttaag ctaatttaat tatttatttt tgcattaact
    31741 acttcatcta ataacttaaa gtcatctttc aaagatttgt atattctttt atacaatcta
    31801 tgatatatat tgtatgtatc tacattttca cttattggtt ctaatttagt tgaagttttt
    31861 attatagcat cacatgcttt ttgtatattt tcatatattc cagctcctac taatgcaaga
    31921 attgcaactc caagtgctgg tccttcactt gaatttatag tatatacatc ttgattaaac
    31981 atatctgctt gcatctgtct ccatacttta ctttttccac ctccacctga ggctcttact
    32041 tcattaacgt ctatattaag attctctatt atatccatac agtctttaag actatatgaa
    32101 acaccttcca ttattgacct tgcaatttct tttttagaat ggattggaga taatccaaag
    32161 aacacacctc ttgcataagg gtcaagatga ggagttcttt ctcccatcat atatggtaag
    32221 taaagcaatc cattacatcc tgttggtact tgacttactt gattatttat aatctcatat
    32281 acatcttcac ctaaagaatt tgaaacttcc acttcacttt gacagaaatt atctttaaac
    32341 cattttaatg acaagccagc tccttgagta actcccataa catgccatgt atttggtatt
    32401 gcatgacaga aagtatgaac tctaccttct ttatcaattt ttggctcttt tgtataagca
    32461 aatactacac ctgatgtacc tatagtagat gaaactactc catctttaac tattccattt
    32521 cctatagctc ctgctgcttg gtctccagct cctcctacca ctattgttcc ttctttaagc
    32581 cctgtcaaac ttgctacatc tctagtcaca taacctgtaa tctcttgtga ttcataaact
    32641 ttacctaaaa gatttttatc tatatttaac ttttctagta cttcatcgct ccaatctctt
    32701 tttcttatat cgagcaactg cataccactt gcatctgata cctctgtagc atattcacct
    32761 gtcaatttgt atcttatata atcttttgga agaagtattt tattagtatt tttatatata
    32821 tctggttcat tgtttctaac ccataatatc tttgatgctg taaatcctgt aagtgctgga
    32881 ttagcagtta tttcaatcag tctttcttca cctactaaat cagttatctc ctcacactct
    32941 tttactgttc gttggtcaca ccatattata gagtttctca atgttttacc ttcattgtcc
    33001 agcataacta gaccatgcat ctgtcctgat aatccaattc cttttataca ttcactgctt
    33061 atatttgaac tttgaactac ttctcttata gattcgacta cagctttcca ccaatcattt
    33121 gcatcctgct ctgcccaacc tgatttctct tgtataagag gatattcata tgtacaactt
    33181 tttatagtat ttcctagttt atcaaataaa actgttttag tacctgatgt accaatatcc
    33241 acacctaata catactccaa cttcatccct ccttgaatgc cacttcataa ctatcgtttt
    33301 cctatcctaa tttttttata ttttaggtat attttacttg ctttattttt gttagtatat
    33361 taaactaact tatgaactaa taataaaata tttaattatc tttgtcaatg caaaataacc
    33421 ttttattttt gtgtaaaaag tattacaata ttattttaag gaggaatatc aaattggtta
    33481 ctgataaata cactattcga gaaatgaatg aaagattggt acttgaacaa attataaaaa
    33541 atggtcctat ttctagggca agtatagctt ctactatagg attgaataaa gcaactatat
    33601 ccgctattac caaaaaactt attgatgaat ctctagttca tgaaatcgga attggaaata
    33661 gtactcatag tggcggaaga aaacccatat tattagtatt taataaatgt gcagggattt
    33721 ctttaagtat ggacattgga tacgactata tattttcttc tctatcttac ttagatggaa
    33781 ctattataaa ttctaagaaa ttaacagata tacaagtaag taaagacaat gttatacaat
    33841 tgatagatga aattataaat agttataaca tatccaaaat agacactcct tataaggtaa
    33901 ttggtcttac tttggctata catgggatta cttgtgaaaa taaagtattg tttactccat
    33961 attacaatct taatgagatt gacttatata gcatccttag caaaaagtat gactttccta
    34021 ttcatataga aaatgaagca aacttaacag ctttagctga gaatactttt tcaactgtac
    34081 ataactcact acttagtctc agtattcaca gtggatttgg ttcaggcatt ataataaata
    34141 ataaattata tagtggcaga aatggtatga gtggagaaat tggtcacact atcatcatgc
    34201 ctaacggtaa actatgtcct tgtggaaatc gtggctgttt agaacagtat tgttcagaaa
    34261 aaaaggtatt tgaacagctt tcttctttag aaaatattcc taaaatagat tctgatatag
    34321 taaagcaact atattatgaa gataaccata atgccaaaaa agttattcat gagttttgta
    34381 gttacttaac aatagctata aataatgcta ttacaactta tgctcctgaa ataatatatt
    34441 taaatagcca aataattagt gatatacctg agatattaca gattacaaaa gatatgttag
    34501 ttagctcttt taacaaagga ataaatatag aaatttcatc attagggtct gaggcatctc
    34561 tatatggtgg ctctgctgta aatatcaaga gctttttgaa tatacaaaat ttaactttaa
    34621 ttaatgaaat ataagggtta tgactatata cagccataac cctttaaatt atacattcta
    34681 aaattagatt tattatatag tttatgcaag attagctata ttttttaaaa attgtatatc
    34741 aattaagtat taacataagt acttcgtttt aaatattaat ataatcttcc tactcatcat
    34801 cagataataa acttgcattt tgttcaacca tagaaatatt atctttacct attttctcta
    34861 attttgctgc caattccttt gtatctaaaa aagctgattc aataaccata cctaaattta
    34921 gccctgaaat aattttaatt cgactgtcat tattggctac taataaagca gcattgcatg
    34981 gagttccacc tagtaaatct gccattataa ttatttctga actttccttg tacacttcta
    35041 aagctttttt taatttttct attgttcctt ctattccatc atcttgtttc atatttacag
    35101 taggataatc tatagaatcc cctattatca tttttgcaga atttaagact tcttcagcca
    35161 tattgccatg actcatcaaa ataatactat tcaatttaat acacctcttt aaatctataa
    35221 gatgtctaca agataagttt tcttatcatc aggcgtcatt atagttgtaa tttcaattcc
    35281 ttcatctaaa agtcttttaa atgcttcttt ctcttcttca gtaacactga tattaggtct
    35341 tagaactgtt gtatcttttc tacttgacat atttcctaca tttatattat gtattttaaa
    35401 tcctgcctct acaaatggaa taaggtccac aggagactta acaattaata atacattttg
    35461 tccttcatac tttcctgcag taatattttt tatagcagtc tcttctgtaa ttatagatga
    35521 cgctatacct gcaggtgtag ccattcttaa aacttgtttt tgaacagaat tgttagctac
    35581 tgcatcattt actaccatta atcttgtaac acccaattta ttactccaca tagttgcaac
    35641 ttgaccatgg attaaacggt catctatacg aatattttta atacctttca tttccatatc
    35701 taatatcatc ctttccttat tttaaaatac ctaatgcata taaaattatt gaaacaacca
    35761 ttactcctaa aattaagtat ataggtttta tattcttttt aagagcccaa taaacaatca
    35821 caactagaat tgcaggaact aacccaggca taatttggtc taatatttct tgacctttca
    35881 ttgtaaaatc accttgttga aagttcaaag caacattagc ttttacaact gaaggtatta
    35941 atgcacctac aaccattaaa cctaaaatag atgtactctc agttaaaatc tttaaacttt
    36001 tttcaattga agatattagc ttagtacctg atgtataacc agcattaaat agtggtatac
    36061 ttaataattt caatgctaca ctcactacaa tccacagtaa gattccaaat ggacttccat
    36121 ctatcgccat attagctgct atagctccaa aaattgtcca cggaataacg acaaataaac
    36181 tatctcctaa acctgctact ggtcccatca agcctgtttt aattgaagca acagcctctt
    36241 cagtctcttc acttgataac tcctccattg ctacattaat acccaaaata taaggggcaa
    36301 cccaaggatt agtgttaaaa aattgtaaat gatttaatac agcactttgt aacttttctg
    36361 gattatcttt gtatactact tttaaaaaag gtagtactgc ataacagtat gccaaggcct
    36421 gcatcttctc ataatttgaa gaaacagaac tagtaaaaaa ccagcgccat aagacacttt
    36481 ttttcacctt ttcatttgat ttatttaatg ataccttatc actcatcttc gtccatacct
    36541 cctactacag tatattgttg ttcttgattt tccaaattct ttttgtaaat tataagtgca
    36601 attgctccac caattaaagc tacacctaat aatggtactt tcatatatac tgctaaaaca
    36661 aaaccaatta ctaagtaact aaaatactcc ttagcaggaa gatatctaag taataatcct
    36721 atccctactg ctggaagtag tcctccagct actgttaaac cacctgttaa ccattcaggt
    36781 gtatatttca aaatggtatt aattaaagaa ggtccaaata ttacagatag agcaactgga
    36841 atacctgttg tagctgctgt taatagtact cctaacattt gcatataggt tatattttta
    36901 taatttcctt ttttagctcc atcttctgct ttatgaacta gccatatacc aatagtattt
    36961 ctaagaacat ctaattgtac catcaataaa gcaactggaa tacctataga gattcctaca
    37021 gatgcttctt gcttagtcgt aatagttata aatgtggcta ctatacttgc tgtttgataa
    37081 tcaggtattg atgctccacc aaagttacta atacctaatg ataatagctg taatgtacct
    37141 ccaatatata accctgtatt tatatctcct aagataagac cagtaataaa acctgctgtt
    37201 actggttgat atgttccaaa cattgttgaa tttttttcat agtttataaa aaatccatat
    37261 agtacgatta ataataattg ccataattgt ggctccatta tgatttcacc tctcccaaaa
    37321 tattttttag ttctttaaaa ttataactgg aatcttgctt taggaaaata ggaatttctt
    37381 ccagtgggca ttcaacttca taagttttcc caccttcata actaactcca ctaaatatat
    37441 tttcccatgt atgtcctttt ggtaaatata cttttctact tctatcttta taattcatta
    37501 ttggagctac caaaatttca tctccaagca tatatgtatt atccacctcc caagttttat
    37561 catcttcagg aaattcataa aataaagttc tcataactgg tgttccatct tcatgcgctt
    37621 ctttcatcag tttttttaaa taagttttta agctctctct aatttttatg aattttgtaa
    37681 gaattacttc aacttcttca ccataagacc atatttcatt aggagctcca gttaccattg
    37741 agcctccacc tttatttgat aatggtttac tatgaggcaa tctatctcca tgcattctca
    37801 aaattggtga aaaagtagca tattggaacc atcttaccat aagttctcta aattctgggt
    37861 ctttaggatt tcctccatgg aaacctccaa tatcagtagt ccaccatggt atacctgcca
    37921 gtcccatatt taaccctgta tttacttgat tattaaaagc ttcaaaacta gaatcaatgt
    37981 caccagacca tactaaagct ccatactttt gagctcctgc ccaagctcct cttacaagag
    38041 ttacaacaga ctctatgtca ttatgtaaac catcataaat catttttaaa taatcaatag
    38101 gataaatatt accacaactt aaaacatttc cttttttata tctatagtta tcaaaatcat
    38161 acacagaata gccaggttca gctacatcta accaataata gtcaatccca agttgtttat
    38221 aatttttatc tattctatcc caaacatatt ctcttgcatt ttcatttgtc atatctacaa
    38281 agactgtatt tccttgaatt tgcattgtca ttctaacacc acgattaaca ttgactaaat
    38341 agccattttc taaataatca ttgtagtttt ctgcatcact ttgaactgta ggccatacag
    38401 atactattgg ttctactgaa tactcttctt tcaatttttt aactaattct ttaggatttt
    38461 tccaataatc taagtcaaat ttatattcac cttgcttagg ccaatgaaaa tagtcaattg
    38521 caattgatga aagcttaatt cctcttttag aatattcctt cacaacatct aaaacttctt
    38581 ctgaagttct atatcttagt ttactttgcc atagtcctaa aaggttttca ggcatcatag
    38641 gaacagttcc agtaacttga gaataatttt tatttaactc ttttgggctt tctccacaag
    38701 taatccaata atctataaaa tttgtagaaa acatcttcca ttctgtcata tttttagcaa
    38761 aagatacttt tccaattccc ggattattcc ataaaagtcc ataacctaga ctagaaatat
    38821 aaaaaggaac tgatatttga gaatttcttt gggctaactc caaaacagta ttctttaaat
    38881 ctaaaaattt atgttgatat tgtcccattc caaaaatctt ttcagaaggc tcagattcaa
    38941 atctagtagt tacttcaaat tctccactat agcttggtcg atattctctt gattttaact
    39001 ttagagtaga gttaaagtct ttggtaatct caattgtccc tacatcttct ccaccatcat
    39061 catgttttac agctcttaat cgaatatatt ctttcaataa tatttcattt ttatcattat
    39121 aaaaagtaat tctatctcta tggtctaaga cagcttttat ctttccattt ttaatgattg
    39181 cacttccatc ttcattttta ttaattgtaa tatcatcttt atttaactta ggtttttcgt
    39241 taagagcata gttttcatca acaaaatttt ggtctacaaa cgacctaata cgtaaagagt
    39301 tttttcccca tgcttctatt cgcaatagtt cattgtcaaa tcttttttca atatagtctt
    39361 cagatatttt tatcacggct acctcctaaa tcaatggtat aatattctat ctttgtgtag
    39421 cttaagttaa ataatgtcgt ttagtatcgt tatttataaa ttaggtttta tatataattc
    39481 atttgtacat taaactaact taccaataat atactttatt tgactttgtt tgtcaatcgc
    39541 tttccggatt ttagtaaaat tttctaatat attgctggaa agtcgttctt aatatctttt
    39601 atttatactt ttaatatctg tgatgatacc tatatacatc tagttttttt aaaacacata
    39661 caggaataac atttgcctat tatgtagata atatcggact tgaaaatatg tatagtgacc
    39721 ttataaatac agataataat atattagata ttgttcaaaa caatggattt tataattata
    39781 aataatttat taaattattt aagaaaatat acaattgtat accaagtcaa aaacgacaac
    39841 aaataaataa aactaaaaaa gatgttgtct caaaatatag ataaatttat ttttcattgc
    39901 taaaatgatt aaaatatatt tgatttaata tacttgattt aatataaaaa aagagtgtct
    39961 cataaactaa aaagttcact tttagacact cctttatata tattatctac ttcttaattc
    40021 atttattacc tctctacatt ttctttcaac tagtggatac atctttataa gtactaatta
    40081 ctccacttaa aaatgattca ggtactgtcc atcagtcata tttttctgcc aaatataatg
    40141 gtgtttcata atgagacaat aaatatttat atctctactt atcttctcta agctcgtcaa
    40201 atccaactat aggtatattt aaattatatt ctttaagtag cttttctagc tcttctataa
    40261 aaataccttt cccatccacc atttgtatat catttatttt atccatcaat tcattgcaac
    40321 cacttaactc accaaaccaa cgtttagaac tacatgtctt attataacca caacttggac
    40381 tgccatctat tgcaatgata ctttttatat cataatcatt ttttaaatat tcatcaaact
    40441 gccttacatg aggtattaac atttgcctac attgttctct aaaaaacaaa ttgtcaaact
    40501 gttcttttac atgtccccac ctatttattc catacataat catttctggg catggtagtt
    40561 gaattattcc atacccttta tttattaaat ctatcgctat attttgtgct cccttaaatt
    40621 ctgacaatcc ctctacttta gaattacaat ttaatataca atgacatact actacaatct
    40681 ctctatttct cttcataata atctccttag ttattcttaa ttttaatgat actacatatt
    40741 ttgataatta tattatttat ttgttctata tttccaatat tctatataga aatatacaat
    40801 tagtaatctt ttataatcta tactatgata aatgtatata tttttttagg aggtattata
    40861 attggatact aaaaaagtca cattttcttc aatgtgcata gttattaata ttgtcttagg
    40921 tatggttata tctatgataa atataccatt attattttta gatacaattg gtactgtact
    40981 tggggctgtc gtattaggtc ctttttggag tgctcttatt ggtgggtgta caaacttagt
    41041 tttaggtgtt atatcaggtc ctacaaatat accttttgcc cttgtaaata ttgttcttgg
    41101 tcttattgtt ggatatattt ctaagaaaaa aggttttggt tataaagaag ctattattac
    41161 aggagtaatt ttatctattg tttgtccttt aattggtact cctatttcag ttttattgtt
    41221 tggtggttta agtggaagtg gtgctgattt attggttggt tttttagttc agtctggtca
    41281 gaagatattt actgctgcgt tctttccaag gattcttagt aatatagtag acaaaccttt
    41341 gtcttgtatt atggtggtat attttataag taaaatgcct aaaggtttta taaaacaata
    41401 tagtaaaaat acttctagtt catattaata aatcatttat tatgtaagat ttattactct
    41461 ttaaaatgct atatactcat ttttatataa catttttatt aataatttaa ttagatagta
    41521 tttgtattat tttaattaaa cgtaacctct tgatataaat ttgagctata aatctatatc
    41581 aagaggtgct attttatttt taatataatt aatattctat tacgcaaaaa atacatatat
    41641 ttcattattt tatggaaaat attacttcat ttaactctat atcaaactca ttaagtaaaa
    41701 atctaattat atttaattta gcctctgtat ttaaatttgt ttctaaatat atattttctg
    41761 atattttaat accttttctt aatttatatg catctctaga cttaatatta tctataaaat
    41821 ctcttttata agtcttgtct acaatactgt taaaccttac agaatccaaa tcatataaag
    41881 taagagcaac ttgttcataa aattctgtcc aatttttagc cttatatgac tctcctaata
    41941 tactaaatga tactatactt tcatttgtaa aattaacatc atcatcctct aacgtaaata
    42001 tattttcact gttacttgga ggttcatatg taacacttgg atatttccaa atttctacag
    42061 ctctatctag taatatctta gttctatttt ttatttcttc ttcattccat ttatctatag
    42121 atgaaatata tttatttaga taaagcctac tatctttaaa tccatcttcc atttctttct
    42181 tttcttcaaa tgttttatta cttagttttg aattatatcc tgtcaaagtt aaatttccaa
    42241 ttgtatgtaa atatttatca tgtatctcct tatagttatc tcctagtgat tccttccatt
    42301 tatttgttaa tgtctgtggc attatatgct ctatatttag catattatta ttgattaaat
    42361 tttctaaatc aacaatttct ttattattat aattctctaa cctctcaagt atatataact
    42421 tattctttga cttagtatta tatatatctt tagtttcaaa agcatttgaa aattcactgt
    42481 cagtaggaaa tctttgagat gatttcttat ttataaaaat atatcttaat atattagaat
    42541 agttttcttt ataatcacta aattttttaa tttcccttcc taagttcata aatactttat
    42601 tcagtgcatt aggaggtaca tcacatacta ttcttcttaa tataaaacat tctactaaat
    42661 taagggattc tacaagtact tttttattta ttataccttc attataatca tcaaataatt
    42721 ctaatataaa cggatatgat acaaatattt caagtttgtt tatccttttc aaaactttgt
    42781 ttacatcatt atcatcagta catgaataag atatctctct ataatagtta gaaaatttaa
    42841 gtaaatcttt taataaaatt tctatatcta taccattatc tgatacatac cttttaaaac
    42901 taaaatatac tttattttta ttcggtatac ttctctcctt tatagtcaaa tagtctctta
    42961 taaaactact tacgttgcta cttgtatttt tttcaatttt ataccaataa ttattataaa
    43021 attcctcttg taacttagag cttctaccca ttaacacaaa gtttcttact ctatcagctt
    43081 cacttaaatc taagccagtt gaatttagac tttcaaagat taattgaggg tcatcttcac
    43141 cattttttaa ctctatttct actatagtta atttctgtat agcatcaaac aaatcatcta
    43201 ttgatatatt atcttctttt attctattat aaaaatataa ataatttgaa actatattag
    43261 aatttctatc tgtattcatt tcttcttcaa atagttcata gtacgctttt ctatcttctg
    43321 ctattggttt taactttatt tttccatctt tatctcttga gtgcttgttt ataagataac
    43381 actccttaat ctgttcttct gttgtaataa ttccttttga ttccccaaac tcttttatta
    43441 tttgacacat ggcaatcaat aatattgaca cagtagtcac tctttgttgt ccatctatta
    43501 ttaaatattc ttttcccata ctatatatag atacaactga ccctaagaaa tgagttctaa
    43561 atccagaatc tattatatcc tctaaatcat caaacaatct tttacattgt tctttcttcc
    43621 agttataatt tctttgatat acaggtacta caaaactttt gtttgacccc tctaaataac
    43681 taagaaattc aatttctcct gctttcatat taactccccc tataagttaa ataaattcca
    43741 ttcataactc catgtatcta tatattatat caaaattttg actcataaat gtatatgaaa
    43801 gccttattat ataccttttt atacctgttt ttattttatt aaagtaaata catgattatt
    43861 ctataaaata tacaaatact taattttaaa aattattatt taatcttctt catgagtaat
    43921 tcatagttat ttatcaatta aaaagagata ttgcataatt attcaatacc tctctttgta
    43981 tatttacatt atccatatct agttatctat ttcaacccaa tattagacta cctactaata
    44041 aatttattct ctacatcatt aacaataatt ttgcactaaa taacatcatt agcctatctc
    44101 tgatgcattt tctttaattt tatttatagt tttaatcaat ttatttctct cttcaatatg
    44161 attttcaaat aaatcaatta tacttccaac agttctaaat gggtcttcca tcaacacttt
    44221 gttatcctct ataaatccat tcttgactac ataatcgact ataagtttaa caaaatgtat
    44281 ttgcttagta ttaaagctct tattatttaa aaactctgaa aaagcttcat ttgcagcttc
    44341 tctatccaat cccactagtc ttctcactaa ttggcttact ggagtatctc caaactcctt
    44401 cttataatca tcacaattac ctaactgttt taagagtatt tcctccaacg tttcaacatc
    44461 ttgctttgtt atctttttat tatttcttaa tttaaatatt gctaattcat ctttatggct
    44521 atttaaataa tactctacct tctttttata attcttaaga ttgtttgcat tatatacaga
    44581 atcatttcta tcttctgcta caatcatatc ttcaaaactt gtataatata tcttttgact
    44641 agacttttca agatacttaa taagctctct taatgatata cgtacttctt ctacctcaaa
    44701 tatatctgaa tcttcccaaa aatcctctgt cattgctttt tcaatagtat atttttgttg
    44761 ctgaatctgt ggtatagtcc caagttttga aagtttttct gctgtagcca ttacatgcct
    44821 tataccttta cttgcattat tattttgtaa gtatgcaagt tgtatattat acataactac
    44881 atcaaatctt ttagccaatt catcatcact atatgaagtt atgagtggtg atatatattc
    44941 tcttatctca ttttgtgcta ctgctccaat attgctccaa acattttcat ttttatactt
    45001 atgtacataa cttaaattca tcctaactat ataactatcc tcatttaaag cttttacaga
    45061 agttataagt tcttctaaca attcttttct aagttctgca tactcacctt ctatatattt
    45121 aatatcttga agttctttaa ccatacttac ctttatatta aataattttt cagtaagtgt
    45181 ttctgtttta tttccctcaa atcctttagg attaaattta aaaaattcaa aattattaca
    45241 aaaatcaaat attaagaatc tttccttatc catatcaaca cctaataaat ctggacataa
    45301 tctagttcct ctacctatca tttgccaaaa cttagtctta cttctcactt tcttgaaaaa
    45361 aacaagattt agtatttcag gtatgtcaat tcctgtatca agcatatcta ctgaaactgc
    45421 tatttgaggt aatttattct tatcagaaaa atcatctatt atagaatcta cataatttat
    45481 actatagtca actgctttta caaagttccc accatacttt ggatacagtt tattaaatct
    45541 ttcaactatt gccctagcat gctttgtatt ctttgcaaaa attatagtct taccaatctt
    45601 ttcttctcct tctattttca acccctctgt cattagctta tttaaaacta aatctattgt
    45661 gtcagaatta aatagccact catttactgc attattccct atttcgtctc caatactctc
    45721 atcatcatta aatgtatttt catattcttc cttctcttca tcagaaagct catcatactt
    45781 aattccatct tccataatct tagtcttaaa ctcaatacta gtataatcta ctaaatatcc
    45841 atcctcgact gccttattat actcataagc atatgtaggt actccatttt ccatgtcaaa
    45901 aactgaataa gtatttttat ctatctcatc ctttggtgta gcagtaagac ccattaagta
    45961 agaatcaaaa taatcaaaga ttgacttata ctttttataa atacttctat gcgattcatc
    46021 tattataatt aaatcaaaat gaccaggtgt aaacaatctc tttccatcct tagacttcgt
    46081 atcatcaatt gcattcatca ttgttggata tgtagaaaat atcatcctag catcttctgg
    46141 gctgtctttg ctatccaata aattacataa cgccaagtta ggtaatagct tagtaaaact
    46201 ttttttagct tgctttacca atgcttttct atctgctaga aataatatat ttttaatcca
    46261 attatgtctt gaaagtacat caactataga tatagcaatt cttgtttttc ctgtaccagt
    46321 agcacatacc aaaagagttt tgcgttgctt attttcaaga gcctcacata cagccctaat
    46381 agcttctttt tgataatatc tattggatat ttcatcattt attgttacag aacttaaaga
    46441 cttcttactc tttcttctat ctatcataag ttgtaattct gattttttat aaaatccata
    46501 aactttccta tctgaatagt catcccaaat atacatatca aaaccattag tcaaaaatat
    46561 taccggtctt tgactatatc tcttttccaa acaatctgca tataatttag cttgttgttg
    46621 accaattttc gggtctttac tcgttctttt cgcctcaact actgctaatg gcttaccatt
    46681 ttcaccataa agaacataat ctacatatcc cacaccactt tcattaggca taccttgtac
    46741 ttctatttct tcccatatat cttttccaaa ttcccaacct gcaagcttta attctatatt
    46801 tatatatctt tttcttgtct caaattcgct tacttcgtct atcttgaaat catagtttac
    46861 agtattaact tttctctttt cagttagtga ctttcttaat tcctcatttt cttttattac
    46921 ttcttcaagc tttttatctt ttgaacttaa tctctcataa agatttttaa gttcgtctgg
    46981 tctcgtcctt ttctcttccc catgaagtaa taaatcttca ttaaagtctt ctgctgtata
    47041 ttcatctgaa taacaatagt ctatccaact aacaaattga tgcaaattgt taagtgaaag
    47101 tattgcttct cctctctcta tgttcgcact agtatgtaca gataaattac ctaatttaac
    47161 tatatatttt aataaaggta acatatcata gtctataagt tctataaaac tattattatg
    47221 aatcaacgta ctaagattat cttgataagg caatactaaa tcactatcat tagcatatag
    47281 ccattttact gcaagctcta aagctcttct tgttatagtt gcacaagtag atgggctaac
    47341 tagtatactt ttttctgctt ctaagcaagc agtattaaaa ctattgaaat ttttcttctt
    47401 ttgtaaaaaa tcaaaattac ccaaggcttt tatccccctt ttgtccctta gttaaaataa
    47461 atctctctaa aaactatttc ttacagctgt ttcttatact aacaaatact tcttcatttt
    47521 ctgtaaacaa ttcgtacata tcttcaaact gtggtatatc atatccatga gaatcatcat
    47581 ttctcaattt tctaagtgtt tctataacta tagaagaatt tttaggtaat aaattgtgat
    47641 actcacaagc taaaaggtag tcataaaagt ttttagattt tatgtttaaa tcaattgatt
    47701 tataatacaa cattgctttt gttaattcta gaaatgatac catatagctt ttaaagcctt
    47761 cttttatgta tctagcccat aactcaggca tttcttcctt atcatataag attttgaagt
    47821 tttgcaggtc atacaagcaa gaaccaaaat ccctatttac ttcttctatt gagttccaag
    47881 ataatttgtt tctaattacc atacatgtcc ctccaatcta agatatctat tacatcactg
    47941 ttttcaaaat agaatttatt tatataatcc ttaaaatctt tagatttata aataacttca
    48001 ccttctaaaa tagctttttt aagtgctatt gtataattat ctgtaacaat ttttaggtct
    48061 acatcaatgt ctaatatatt tcttagagct tctgtcttag ttgcaagttc tgtaattggt
    48121 acttttttga tgggaatcca agctatgtct atatcacttt ctgatgtatg tttatctgtt
    48181 ccataactac caaatattat gattgctaga gtattttctt ttggaaatac atttattatt
    48241 gagtttttaa tctcatttgg tatatttctc atttaatcac ctcttgttta ttaggtaata
    48301 ttgcttaatt gaatttatat ccataatctt ttaatctatt tatacatatt tctctttctt
    48361 catcagtaaa caactcttta tacttttctt ttaatactaa tacttctaat gataaatcta
    48421 atctcttaca ctcccatagc cttgtaaatc cttcagtagc tccttcttta ttgattaatt
    48481 ctttagcagc taataaggct cctttgttat ttatcagttg taaaaatctt gttggcttat
    48541 agttacattg taaatcagct tctttataga tatttaccat gttatggtat aaatctagct
    48601 ctatttgatt catagatttt attcccccat tttaataatt gtattttagt tgaataacta
    48661 tcctttaaaa gctttttgca ttaatgagtt aaagttgtct tctagttctt ttagacttgt
    48721 ttttattcct actttcaatt tgtctacttg attaacaaac tcttcaaact tattttgaag
    48781 ttctattgat ggtactggta ttaatatttt ttgaagttct ttgcttctaa ttccttgtgc
    48841 agagctacca gttttatatt tatctatttc taccctaaaa gcttttgttt gcatatattg
    48901 tgctaaaaaa gcattattta aaattttttt atctccctga attgttataa tcctttgacc
    48961 catagcaaac ttttttaaat cctttggtat cctacataca tttccaaatg tatgaccttc
    49021 tgtaacaaaa agtacatctc cagctactgg aaaacctctt ttcatccaag aattataggt
    49081 gttttcagat atatattcat taatatattt aaatacacca ttccctacag attttgcatt
    49141 tattattcta attccattct ctgaaagaat tggagtttta ccacgataat caataaaatt
    49201 tgtacaatct ttcaataata caaacttttg ttccttaaaa tttccaaaca tttcaataaa
    49261 tcttgattta actaattcat ccaatgcttc tatttgctct tttcttttat ctattaactc
    49321 ttgagcttta tctaaaactt ttgatatatc tttttgagct tccatttcag gtaaatctac
    49381 ttttaaattt tttaaatcat taactgaaaa acttgcttga ttaactgatt tttttattat
    49441 ccttggtaac tgtcttctaa attcattact attaaaaaaa taatttaaat actttggata
    49501 acaaacttct ttatctggtc gtaaacataa aagattcatt ccatgtataa tttttttatt
    49561 tatatcttca tatattgcac ttttccctaa atgtttttca ctattaatat gtgacattag
    49621 tatatctcca tttttcaata aatagtcttc taattttcct aactctttta tgtcagcata
    49681 tcccatttta ttagtatcaa tataagagtt tgctatagtt tctattcttg taattggata
    49741 tccaaatttt gtatcaaatt gtttaatact agccccattt ctaataaatt caaaaacttc
    49801 attcaatcta tattccatac tctacacctc aagcattctt tctaattcct ctaatccatc
    49861 tacgatttcc ttctctaact ccctaactct acctataata acactaggag catcataaac
    49921 tacttcctca tacactacct ctttatactt attaatagat aaatcatacc cattctctct
    49981 tatctcttcc acatcaacaa aaaagctttg ctctgttctt tttctatcta cttcaccatc
    50041 aagattatca aatctcttaa taatatcctc tatatcacta tcctttatct catttctctt
    50101 atcatccaaa gaataaccat cagctttcat atcatagaac cataccttat cagttccacc
    50161 tgttcctgtt ttagtaaata tcattatagc tgtagatact cctgcataag gcttaaacac
    50221 tccacttggc attgatatta tagcttccaa tttatgatta tcaactattt cttttcttat
    50281 cgatttatgt ccacctgttg aaccaaacaa tactccatct ggcactatac atgcgcatct
    50341 tccaccaatt tttaaaactc ttaaaaaaag tgccaagaat aataactctg ttttctttgt
    50401 cttagtcact tttaataaat ctgctgacac tgcttcataa tctaaacttc ccttaaaagg
    50461 aggatttgct aacaccaatg taaacttatc tttatctgta ttaacctccg ataaagaatc
    50521 cttatactct atattaggat tgtcaacgcc atgcaacatc atattcatag cacctattct
    50581 aagcatagtt ctatccatat caaatccata gaacatacca ttattaaaat gctccttcag
    50641 cccttgcact aagaaaagac ttgaatgatt atcccttaag tattgttgtg atgatactaa
    50701 aaaacctgcc gaacctgctg ccgggtctac tataatatct tctggtgttg gtttaataag
    50761 tcttactatc atatctatta tgtgtcttgg agtcctaaat tgaccattag tacctgctgt
    50821 agccactttg gataataaat actcatacaa atctcccttt gtatctctat ccttacttaa
    50881 ttctaaatta ctaataccat caactatttt agataacata agaggcgtag gaattttaaa
    50941 tattgcatct cccatgtact ttgcataagc tgattcttta tttccatgta atttttttat
    51001 gaaagggaac acttcatttt gtactatttc atacattttt tctgaatttc cttcattaga
    51061 aaacttactc caccttaaat gttgatgttc atcatcaaat atcttttcaa aagaaacacc
    51121 caaaagagtt gcttctgctt cattttttgt ttctacttca tccaatcctt taataaataa
    51181 aagatatgta aattgttcta taacttctaa tggattagtt aatccaccag tccaaaaagt
    51241 ctcccaaatc ttgtccactt tactccttaa ttctcctgta atcatatatg actttccctc
    51301 cagtgttcat aaaataattt aaatatatat tttagtcgta cgccttaata gttagtgtct
    51361 tatgttaaat ccaagttttt actacacaat tattaaatta tttttcctat ttacatattt
    51421 tagctaaaaa actaatgcaa tactcttatt ttatgattat tatatatacc atacaaaatt
    51481 attttatcat atatccatca tagaagtcat ctacctggat aactcttacc atattataac
    51541 tttttaatat taatttaaac ttagaaagaa attttttatt taattatatc atcttcctta
    51601 tttcaaattt ctcaattaca atcttaatca aaaaataaaa taagatgctg tctcaaaata
    51661 gatataatct tcttttttat tgtcggaatg atgaaaatat atttaatttc atacaaaaaa
    51721 gagtgcctca tgaactaaaa gttcattttg agacacccct taatttaatc ttacttattc
    51781 tttcccttta ttattaacac ttcactatct ctattgcttt tcctacacat ccacaaactt
    51841 ccatcctact cttaacaaaa tctttaactg actctaaacc ttttttacca tattttttag
    51901 ggtcaattac ctctgggtca gaattaagca catccttaac cccattgcta taagcaatcc
    51961 ttaattcagt tgcataatta accttgcaaa tccctctttt tatagactcc ttaataacct
    52021 catctggtat tccagaccca ccatgtaata ctaatggaac tgatacaacc tctctaattt
    52081 cagataatct atctaaatca agttttggct ctcccttata tagcccatgt gccgtaccta
    52141 ttgctactgc taaagatgat acccctgttc tctctacaaa ctcttttgct tctaatgggt
    52201 ctgtatatgc tccaccatca ccaccatcta aatcgtcttc cttacctcca actttaccta
    52261 attcagcttc aacaggtata tctgaaggca tacatgcatc tacaacagat ttagatattg
    52321 aaatattatc ttcataactt tcatgagaac catcaatcat aatagaagta tatccaactc
    52381 taagtgcctg catagctagt ccaaagcttg aaccatggtc taagtgcatt gctactggaa
    52441 cacttgcctt ctctgatgct acctttacat tcgctaaata ataatctagt cctgcatact
    52501 ttattgttga aggtgttgtt tgcaatatta ctggtgattt aagttcttca gcagcctcta
    52561 ttactgccat aaccatctcc atattttcaa cattaaatgc acctactgca taatgccctt
    52621 cttgtgcatc taataatatt tgtttagttg tcactaatgc cataatttct cctcctatat
    52681 gtttatactg atttaatttc ttcgttattt aattttgata ctatttctct acttgttttg
    52741 ctatttaata actcatttaa aaattcttca ttcattaatt ttgaagatag ttgagataat
    52801 gtttttaagt gtaaattatc accattatct gtagctgcta tcataaatat aatatgtgcc
    52861 aagcttccat cttctgattc aaagtcaaac ccttcttttg atagacctac tgctacacgt
    52921 ggtaccttta ctgcatctgt ttttgcatgt ggtatcgcta tccccatacc aaaacctgta
    52981 gatgatattt cttctctttt aaggatttct tctttaaact tctttttatc taaaagaaca
    53041 ctagatttat caagtattcc aatcatttca tcaattacat ctgttttatt ttttccctta
    53101 acatctaaaa ttactgtttc ttcgctatat aaagagatat tattagatac gttttccgat
    53161 tctgcacttt catttttatg ttcatctttt gttacatttg tattttctat agtatttttc
    53221 tcatttacta ctttaggtgt aaccatcata taaagtacag cacctactaa tgaacctaca
    53281 aatatacacc ctacccataa aagtggctta ttgactaatc ctagaaccaa aaaccctcca
    53341 tgtggagctg gtacttctat tttcattata taagttaaaa ttgatgatat tgatgatgca
    53401 atcataagta ctggcaacac ttttaatgga ttttttgctg caaaaggtat tgccccctct
    53461 gttatatgag tactacctag tatataattg attaatcctg ctgctctatc ttcttctgta
    53521 aatctatttt taaataaagt agatgctatt gctattacca atggtggtgt tatacaagct
    53581 gcagatacac ctgccataaa taaataattt ccctgtccta gaagcatagt tccagttaca
    53641 tatgctgctt tatttactgg acctcccata tcaaatgctg acatacatcc tattactatt
    53701 cccaaaatta tagggcttga attttgtaac cccgatagaa agttcatcat agcattgtta
    53761 agtgctgaac atggcccacc aagtaacaac attgctacac ccacaagacc tacgctgaca
    53821 ataggaatta taaaaatcga tttaagacct tctaattgtc gtggtaattt tcttaacaaa
    53881 tgtacaaata gtttaactaa gtaacctgct aaaaatcctg atatgattcc acctaaaaac
    53941 cccgaacctg tatcaaatga tatcattcct ccaacaagtc ctgccaacat accagctctt
    54001 cccgatatag attgagctat aaatgctgcc aatacaggaa ccatcaagtt catagaatat
    54061 ccaccaacag attttaatgt agctgctatt tgattatatt gttctgaatt tgggtcaaat
    54121 gaataaatcc cccataaaaa tgatatagca gtaagtactc cacctgctac tactaaaggt
    54181 aacatgtgag atacaccatt cattaagtct ttatacaact tttgaccaaa cgataaattt
    54241 ccttcttctg caattttact gcttgataag tttccttttc taatagctac ttttccatca
    54301 atacatcttt gtataagttc attagcttta tgtatacctt ctttgacagg tacttctatt
    54361 actggctttc cattgaatct gtccatatca acatttttat cacaagcaac tattattgca
    54421 tctgccattt cgatatcttt tgctgttaac ttattttcta cacctatagc tccattcgtt
    54481 tcaactttta tgtcacaccc taattccctg gctgaatttt ttaatgcttc ttctgccata
    54541 aaagtatgag ctattccagt tggacaacct gttaccccta gaatcatttt ctttcccatt
    54601 tttataccac ctttctgact cttatatttt ttatataatc ttccacattc tccaaattac
    54661 ctataccatt actttcagcc acacttgctc ctgttgctgc tgaccttttt aatgcttctt
    54721 caatatgttc tggattttcc aaccatatac ttaaaaaagc agctaatgca gaatcaccag
    54781 cacatgcaga gctgactact gtaactggtt gagcactagc ataataaatt gattttccat
    54841 tataaaaata agaacctttt tctcctaaag ttagcaatat attttgagca ccttttaaat
    54901 gtagtaattt aagtacatcc ttaatatctt cttcatctct tacaataatt ccaaatatat
    54961 ctttgatttc ttcatcattt ggttttatca aatatggatt atactgtagt aactccttta
    55021 attttggtga acttatatcg agtattgtct ttatactttt attttcacaa attttaaata
    55081 tatcctcata gtaattatca tcaataccag gaggtagact tccacttatt gataagtaac
    55141 ttatatcttc taatgactgt atcttatcta gcatatctag tttttgtttt tcgtttacat
    55201 atgacccaga gtttacaaat ttaaattcat cacttccatc atttaaaaat atatttatac
    55261 gagttgtatc ttctacccaa gtaggcaata cgtcaaatcc tctcttttcg ctttcttcaa
    55321 ctatatattt tcctgaaaat cctccaaaaa atcctaatat cttagattct ataccataat
    55381 gacctagtac aaaagttaca tttaatccct ttccattagg agaatataca gcatttgatg
    55441 tacgatttac taattttctc tttatcccat tagtagatat attcatatct attgcaggat
    55501 ttgtagttaa tgtatatatc attgtcttcc cccttgcttg tgctgtgtat gttttcatta
    55561 taataaaaaa gtctatgaaa atcttttcac aaatagaaaa tcgattttca tagacttttc
    55621 aatttaattt tcagaattat tatatctgac catataatct ataattatcc tagatataat
    55681 atacaagggc aatctggaag atacttcata ttcagaaatt gatatatgtt catgtttgta
    55741 tcctacaata tataaatccg aatactttat aagttttgca ctttcactac aacagcaagt
    55801 tataacctta gctccaacta aattggcatt ctgtgctgat tctacaagct ctattgtatt
    55861 tccattcaat gaaaatataa ttataagttc gtcttttttt attgatttag attttatttt
    55921 cattatgttg gggtcattta taaaaacaac attaaaatca agtaattgca atttaagact
    55981 aaactctttt gctacatatt cagtaagccc tcttgctaaa atatatatct tactagattt
    56041 atttacagtt tttattatat tgattagtgt agttaaagat atttgttcta taactctctg
    56101 tgcttctata aaggatttat ttattatatc ccccatattt tgtatatcat cattttcatc
    56161 ttttactgtt aatctatacc taagttcatt gaatccattt aaaccacatt ttctaattgc
    56221 tcttgaaacg gttgctggag atgaatatgt ttcatgagca atctctacta tagataattc
    56281 tggtaatatt gattcatttt catttataaa tttaattatt tcaagttccg tcttggttag
    56341 attttcagaa ttacttttgt ttagctcaat tatcatatgt tatctcccct tatataagtt
    56401 ttatttgtct aaatttatcg agtaatataa tatattacct cttaactact tatttttata
    56461 tacaattaag atttagtcaa tgtctatata tgtaaattta taatataata attttttgct
    56521 taaagtttat tatgttatct tatagcattt taaaataaaa aagaattgta cctctaccaa
    56581 aatttggatg aagtgcaata gatattcctt atactgatat gtttatgatt aaaagtataa
    56641 atataaattt cgctatgaat catataatta ataaatcatt ttatttacaa ttataacccc
    56701 ccttataaaa ctatattaat aatatataca gtatttataa ggggggttgt ttatatttaa
    56761 aaactattta cttttctttt aattcttctc cattagttct tattacattt tgataccaat
    56821 aaaaactatc ttttttataa cgttttttac ttccatttcc attgttatct atatctacat
    56881 atataaatcc atatcttttt tccatttctt gagatgaaca acttactata tctattggtg
    56941 accacataca ataagctaat atttcggttc catcagctat agcttcacct atttgttcta
    57001 tatgttttcc taaataatct attctgtaac tatcatgaac ttttccatct tcaaatttat
    57061 catgcattcc aaatccattt tcagctatca ttattggctt ttggtatcta tcataatatt
    57121 gagataaagt attgtataat ccttttgggt caattgccca cccccaagga tttgctttta
    57181 aatatggatt ctttatattg tcagttggat tcatggtgtt tttacttgct gatactgttg
    57241 tagaataata gtatgataaa gctagaaaat ccatagtatt ttcttttaag attttatcat
    57301 caccttcctc cataactata tttatgttat tttctttgaa aaatctctta atatagactg
    57361 gatactctcc cctaaaactt acatcagtga aaaaatattg tagcctattt ctttttaaag
    57421 caaatactac attttcaggg tcacaatctt ctggataaga tgtacaatct gccatcatca
    57481 tacctatcat catatctgaa tttatttctt ttccaagctt cttagtcaaa gctgtagcta
    57541 tgaattgatg atgaatagct tgatatctag tctcttctac attatctact tggtctacac
    57601 aaaatgcaac tgaattaaat gattcataat gtattaaatt tatttgattt ataactatcc
    57661 aatatttaac tttattttta tatctattta gtattaattc tgcatatcta acaaagaaat
    57721 ctaccacttt tctattagac catcctttat attttaatac taaattcaaa ggagtctcat
    57781 agtgaagtag tgttacaata ggttccatac cattatctat tacttcattt ataagattat
    57841 cataaaactt taatccctct tcattaggct ctaaatcatc tccatttgga aatattctag
    57901 cccaatttat tgaaaatcta aaacacttta accctagctc tttcaaaagc tttaaatctt
    57961 ctttgtatgt atgataaaag tctattccac tacgcttagg ataatatcca ttattatctt
    58021 tcatagcaaa ctttattctt tctatagtca tatcagactc ataaggttta gatatatctc
    58081 tatctttatc atacatatgt acatcagcta aacaccaacc tttgccacct tcattccatg
    58141 caccttctaa ttgattagct gctacagctc ctccccataa aaaaccttta ggaaacttac
    58201 ccaatttaat cactccttat tttatttatt taaatcattt actattattt accttatatt
    58261 ctatagactt tactattcct actaaagaca tagttatatt taactcgctg aatatggtca
    58321 taagagtatc ttgagcatgt gcaaataaca gcgaatgtgc tcttttctct ccaccagctt
    58381 ccccttgtat aatcttggtt tgagctctat gtgcttctgt cataaaccct tttgctttag
    58441 ctagttttaa atcagcattt ctataatcaa acttttccaa atcattaaga gcatgtttaa
    58501 tttctgttct agcctcacct gcacgaagta ttatttgcat tgccacctct actaattctt
    58561 catctattat atgttcttca attgtcatat ttataatttc ctttcaattt ctaaatttaa
    58621 acctcttggc tctctaattt tatctcttct ttctcataag ctttaaaaaa tggataccat
    58681 atcataaata gcaatactat atttattgct aaaacaataa gacctctaac atcttgattt
    58741 accgtaaaag tagatatacc aaaaggaaca taccataaat taaacacctt acttggaatt
    58801 gtcacaaatc ccatactcat tgataagtaa gtagttattg atattactac tgttcctatc
    58861 cacataggta acattaaaaa tgggttccat gccactggag ctccaaatat tactggttca
    58921 tttatattca ttatagaagg gacaatacat gctcttccta ttgcttttat tcttccaact
    58981 ctggataaac acatcattat aactaatggt aatgtagcac ccaaccctcc taaagttatc
    59041 caacctgttt gaataacttc gtaaacatat atattagttg ctactcctcc tgatgctact
    59101 gctgctgcat tagctgcaat tgcaccaagt tgaattgaat aagttatagg agatagtacc
    59161 cacccactta ttcctaaaga atatataaat actactaaaa agtttaagag tacaaatcca
    59221 ggtaatgtct gtgctatatc tgatactggt ttaaatactc taactattat ttcaaacatg
    59281 tcaaagttga gtccataaat taatccccag cctacaaaca ttatcactgt tataggtaac
    59341 atattatcaa accacttgct tacaaaatcc ggaagtgctg aatcttctcc gaaaaatgaa
    59401 aaagaaccaa ataaggacat tattaatcca gagaataatc ctgcaactag cgatacaagc
    59461 attccttctc ctccaaacct gttgaagtta aatatagctc cttcattcgt aatctctgga
    59521 cgaatcatca ttaacattaa tccagcacct gcaagaccag cgactacact catactttca
    59581 aatttcttat tcttcattat ttccataggc aataaaaatg ctataaataa tcctaaaagt
    59641 ccaaatgtat attgatttat tggacttaaa tcaggtaaaa agcttatgta attcttcaag
    59701 acagaaacta tagttactaa tgagcctaca aataccattg gcaaaacttt cattatagtt
    59761 gattgaactg attgtaccca tatattctta gtcatattat ttacaacagg agtaaatttt
    59821 tcacttaaaa aatctataaa tttattcata aacactctcc ccataaatat tttatttgtt
    59881 tgatattagt tctagtgctt cttccaaagc ctttgcccca tctagtcttc catatgcagt
    59941 tttagatata catgctactg gaacgttata ttcattagcc ttttgttgaa tttcttcctt
    60001 catataatct aaatgtggtc ctagtaataa acaatctatt tctgataaat acttatctat
    60061 ctctgactca cttctcgctt ttatatctgc ttctatacct tttttctttg cactctttct
    60121 catactagtt gccataaaac ctgtacttgc tcctgaacca cacgccaaaa gcactcttat
    60181 atttttcata atgatacctc caatattttt atccatcaat ttatttctta atgaactttt
    60241 tattttatta tgtttatatt gtacttacca aataagttca tttcacataa atatatttcc
    60301 taataatagg aaatcagtta ctcttaatta aacatattaa tatagtataa tgtatcttat
    60361 aatacgtaca ttcaattata taattatcat atcatccaga ttttatttta aaactatgcg
    60421 agggaatcta atgaaaacat ctaaaaaaga actattttta aatttcataa aaacacaaca
    60481 tgattggata gattcatcta cattggctaa ttatcttaat gtctcaacgc gttcaattag
    60541 aaaatatgta aacgaaatta attccaatgg tgaatttata ttatcctcta aaaaaggata
    60601 taaagtaaat ctcaataata attgtcaaac caaggttgat agttcagaaa atatatcacc
    60661 tgataacagg ttaaatttaa ttttaaaaga acttatagtt aatagtaatg gaattaatat
    60721 atttgattta agtgaagact tgtttgttag tccagctact attgaaggag atatagtcaa
    60781 agctaataaa tttataggaa gttataattt aaagataaag caatcgaaat ttttattgaa
    60841 actaattgga aatgagtctg caaagcgcaa attaatgagc tctataatat ttaaagaaac
    60901 gggaagtgat tttttaagct tatttgatgt acaaaaaata tatcaagagt ataatttgac
    60961 caaactgaaa gaaaatataa tatatatatt aaaaaaatac aatttgttta taaatgagta
    61021 tgctataaat aacatacttc ttcatttaat gataactata gacagaataa aaaagaataa
    61081 ctacatagaa tctgtcgaag tagtaaacta catagataat aataatgata taagcatttc
    61141 aagagatata tcaaattttt tagagtctga atacaatata actttaacta gtgcagaatt
    61201 atactactta gtatttcagt taacaaacaa aaccacagtc ttaaattatc atgaaatgga
    61261 tactaagtca ttgccaaatt atatagatga acattttgtt aaattaacta aaaaaataat
    61321 taaaaatgta tatgacttat actttataga tttatctgat gatgaatttg ttgtaaaatt
    61381 tactcttcat gtaaaaaatt taatcagtag ggctaaaaat aatcaagttt tgagaaatca
    61441 aatacctcag aagttaaaag attcctaccc tctgatatat gatatatctg tttatatttg
    61501 taatcagatt caaattttag aaaacataga aatagatgaa gatgaaatat cgtatatatc
    61561 tcttcatgta ggttcatttt ttgatagaca aaaattacta gaagataaaa ttctctgtgc
    61621 attaattact ccaaattact atgatttaca attcaaaata gtaagagacc ttgagaagag
    61681 atttaatgaa tccatagaaa ttattcagat tttttcagat actcataatc ttgattttga
    61741 caataaggta gacatggtta ttactacttt accaattagc gatagatgcc ctataccttt
    61801 tgtttatgta aatccttacc taaatagaaa ggattatgat aatattcaaa gtaaatttat
    61861 tcagattaaa gatagaaaaa atatacttac tgtacaaaat catttagaaa tgtactttag
    61921 tgaatctttg tttatgaaaa atatatattt agatagtgct aaagattata taagatttat
    61981 gggaaatatt ctgtacgaaa ataaatatgt caaacccaat tatatagatg atgttcttat
    62041 aagagaaaaa atgtcttcaa ctgcatttaa taacaatgta gcaattcctc atagcatgaa
    62101 aatggatgct ttaaaaaccg gagtatgttt aattgttaac gataaaccag ttaaatgggg
    62161 tgaggaaaaa gttcaaatta tagctatgat acctataaat gaaaaagaaa aagaaaagtt
    62221 caattacata tttgaaagct ttattgaaat attatctgag tggaataatg ttaaagaact
    62281 tactaaggca gataactata gtagttttat gaatagaata gcttatctta ttcaaaatat
    62341 ctaaatcaat taatttattt ccaatatatg tttcttaagt tattaacata aaatagataa
    62401 gctgtcacac taaaaagtct aaattaatat gtgacagctt tttacctaat ataccttgtt
    62461 ttaatactac atattttttg actgttgtat actagttata agattgaata tttgtgttgt
    62521 tgtagctgtc atattctcac tagcagttgc tttatccatt gcttcttcta ttgtcatagc
    62581 tctatttact atagaaaaat aagcatctat accctcttca ttacatttaa ctgcatcttc
    62641 tgttgtggct ccagccaaac ctattacttt taccccatat ttttttgcca gttttgcaac
    62701 tcctattgga gccttgccca tagcagtttg attgtccaat ctaccttcac cagttatgac
    62761 aaaatctgca tcttttaact cttcctctaa tttaatttct tctaatatta tttttattcc
    62821 agattctaat tttgaattta aaaatgctaa aaaagcaaaa cctaatcctc ctgctgctcc
    62881 tgctccttct acattagcta catctttact taagtaattt ttaactactt tggaaaaatt
    62941 tctaagccca ttatctaact cctcaattat ttctgatgtt gcaccttttt gagacccgta
    63001 tatgtatgca gctccatttt tcccaaacag cggattattt acatcacagg ctattttaaa
    63061 cttacattca tctaattcct ttaatctatt ttctattttt atatgtctta tttcatttaa
    63121 aacttttcca cctaatccta ctaacttgtt attttcatca taaaactcaa accctaaggc
    63181 ttgaagcata cctatcccag catcattagt agcacttcca cctatcccaa ctataaaatt
    63241 tctatagcct ttatttattg cttcttttat tatttcccca acaccataag ttgtagtgta
    63301 taaaggattt ctaagttcag tcggaaccaa tgttagtcct gatgcttgag ctatttctat
    63361 tattgctgta cttgtatttt ttaataatcc atatgttgca tccacttttt tagctattgg
    63421 tccagtaact tctatagtat cttttttacc attcatccct tgaactaagg catctactgt
    63481 cccttcacca ccatcagcaa gtggcaatat ctttacttgc gcatcttctt taaccttcaa
    63541 tatccccttt tttattgcat ttcctgcctc tattgaagat aagcttcctt tcaaagaatc
    63601 tatagaaatt aatattttca tatttatcta ctccttttta ttactcctta ttttaaacat
    63661 ataattttat aagcttaatt tatcttttat tttatactct acatctctct ttgtatacaa
    63721 cttataccat taatattctt ttatcttata aaaagggagt gtctcaaaat aaatttctta
    63781 gttcatgaga cactcttttt gtataaaatc aaatatatgt ttattatttc aacaatgata
    63841 aggaggatta tctctatttt aagataattc ctttttagca ctatttaacc agtatagttt
    63901 taacttggtt cattaagcac tctacattta cagagttatt atctattgat atagattcta
    63961 attctttttc atttaggtta atccatttag ctgttttaat atttttgtta aatactatgt
    64021 tagctgattt tgtttcttct ttagaattgt aaaatctaac tactaatgcc tcttgttttt
    64081 cagccttttt tacaacactt aacacaaggt ccttattagt ttccttaagt aaactatatg
    64141 aataatctac ttctatttct gattgattca atttcatagc attatatggc attttattat
    64201 aagtttgtat ttttgttaaa tactctttag ccatatttgc aacatttgct tctagtgtag
    64261 atttttcatg agtaactatt gctaagtcta tttttatatc tcctatcatt tgagaatctg
    64321 gagtaggcaa ctttattcct gatggacgac ctgggcgtct tgccatttct tctttaccta
    64381 ggaatcctac acttctaaat aatgttatag ctattgtgtc aaatttttcc cctactattt
    64441 caaactctct tgtactgttt gtaagaacag ctactccatg ctctttgtca gacaatccaa
    64501 cgaatgttag cattggatat atagaatctg gtctttcact ccattcttcc tgttgccaaa
    64561 cttccatagc atcatcatac acacttctct ttatatgtcc aaactgatta tcagacacag
    64621 aaaactttga agctatattt gttggtatat aagttctaac cctacaatct cttgcttggt
    64681 tatttatgtt gaatactaca tctattatag ctttatcatt tggaacattt ataactatat
    64741 caaagtcaat aaatgaatct gctctattat tttttctagc ttgtatatct tttggtactt
    64801 ttaatctata atttatcttt atctcattat taaatctatt tttctttata gtggaatttg
    64861 ctactatatt atcgttaaat attaatttct catttggaag tggtgaaaaa tcatactcat
    64921 ctccttcatc tcctccattt tccatcaata atacattttc aaaagtttta tttaattttt
    64981 tatcaaatat ctttaatgtc ccgttttcat taacttgaat agtataaaag tcattatcta
    65041 ccatgtcaca tacttcataa gctttttcta acatatgttc aacttctttt atcatatacg
    65101 ccttataccc cattgcaggt attttatctt taaagtttat tgtatatacc ataaatgaat
    65161 catagtttcc ataatgaaca atttgacggt ctattaatcc tgcatctatt acttctttat
    65221 gtaccacttc aaaatccaac ttattattat ctttatcaaa tatttcaaat gctttcattt
    65281 tagttataac ttgagcgctt actatttcat tcctatcgta aggcattaag ttaaatgcta
    65341 ctaacttatc taaagccatt tcacaactca ttgcatcaac tattcttctt ttgtaaaatg
    65401 ttattaattg gtctactttt tcctcagcta agaaaaatct attcattatt tcatggtgaa
    65461 ctttatctga acaacaacat ccaatgctgt catgagcatg atttttcatt atttctttcc
    65521 aaattaactc tattaatcca tgatgatatt caaatcctag agaatatgca atagaagcta
    65581 gtggctccaa tatgttggtt atctttgatt ctattctagc atttgctgat tttatatcca
    65641 ttcttgttga gaatatactt ctatgaaccc tcatatattt cccatctaaa aattcccctt
    65701 ttattgtatc tatatcctca tttttttcta gctctttaaa tatgttttca tatctactta
    65761 agaaaaattt tctctctgga taacattctt ttaatttata tataacttca aatatattct
    65821 tttgaattgg catttggtca tgtccatttg gaagtattat atggtcagta gttgcaccct
    65881 tatcaagaac cggtaaatat ttatccattc ttgttcttag ctcatcttca ttcattggta
    65941 gatattttcc tattgcataa cctaatggaa gtatttgaac caaaacagaa gacccatcat
    66001 cactcttcca attaaactct gttttattag ttccttttct ttcagaacat cctctccaaa
    66061 acattgagta ttttatatca aacccattta gtatttgtgg catttgagca ctttgaccaa
    66121 atgaatctgg taaatatcct attttcatat attctccaaa ttcatcacaa tcttttattc
    66181 catatagtaa atttctaagt atggattctc caccaactac catttcatca gtttgagtat
    66241 accaaggacc tattattagc cttccttctt gaactaattt ttttattctc tccttacatt
    66301 caggcttaac tgctaaataa tcttctaata tagcagtttg accatccatc acataatatg
    66361 gatagttatg attattttct aacatatcca ttatttcttc catattattt acaagtaata
    66421 ttctagattc ctcagttgag aagtaccatt ctctatccca atgcatatga ggaactatgt
    66481 gtacattttt tttcattgct tatcctccaa aacttatctg ataatatttt ctgtctaatt
    66541 aaaaatccaa taaaaatact actgagttta caaaattttt taatatttat tttatcacta
    66601 taattaaacc tttgatttaa atatctaata acgtatcttt taatgtatct ttctttgatt
    66661 tttttgtttc ctagttatta ttaataatac tgctgatatt atagctccaa gtactgccgc
    66721 aaagaaccat attgatgcta taataaatcc tgacatactt ccctctaaaa gcgccaatga
    66781 gaatatcccc gctccaggca cttgaagacc tatattgaag aatgctacta tagacccagt
    66841 tatggctgaa ccagctacca atgatggtat aacacggatt gggtcctcta tcataaatgg
    66901 tattgcacct tcagttattc cagccaatcc taatatccaa gtttgctttc ctatttcctt
    66961 ttctgattct gaaaataaat ctctgcctaa aacagtagct attgtaactc caaaagctga
    67021 aaccatctta actgatgcaa atattgcata aggtataaaa tttccacttg ctattgctcc
    67081 tattgcaaat gtataggctg ctttatttat tggtccacct aagtcaaatg aaaccatagc
    67141 tcctattatt gcacctagta gtattgcatt tactccagtc atcccattta accaatttat
    67201 aagaccattg ttaagtgctg ctacaggctt tcctactaca aataacatca ataaacctac
    67261 taccaaagaa ccaactactg gatataccca aaaacttacg aatccagcta atgtgccttt
    67321 ggctggtata ttcttcttta aaaattttac tatataacca gcaagtaaac ctcctaacat
    67381 acctccaaga aatccactac ctattaaatc agctgctaga cctgctgcaa atcctggtcc
    67441 aagacctggt ttttcagcta gtgagtaaga catatatgct gataatactg gaaccatcaa
    67501 tgtacctaac atacctccag aaagctttct taattgccat aaccaagaac cttcttgatt
    67561 gtatagttct tgtagaccaa atgcttgaga tgctaatact gcaaatgcaa gtatcatacc
    67621 tcctgccaca ataagtggta ttatatgaga tactcctgta agtattgctg attttacatc
    67681 ttttttccat gatgattttt cacaatatga tatatcagat gatttttcta tataatcacc
    67741 tttaccttgt ttttgtgcta cttctaaagc ttcgtatatt atttcttttg ctctttttag
    67801 tggagctgct actgacgttt ttatgtttgg caaatgttca aatctttctg tatttttagg
    67861 tgctacatct gttgcatata ttattgcatc tgcccttttt attatatctg gtgtatgagg
    67921 gtccaccata ccattagctc cttgcttttc tacatacaca tccacaccga tttctttacc
    67981 agctttaatt aatgcttctg ctgacatata tgtatgtgct atacctgcag ggcatgctgt
    68041 tattgctaat ataatttttt ttgattcatt ttgaatccta atctcttctt ttatttgttc
    68101 tttcttttct tttatatcta agttattaaa taactctttc ttagtttttg aatttaacaa
    68161 catattcata taatcttcat ttgacagtct agtaactaac tcagaaagta tttgtatgtg
    68221 tgtatttcca ccttcatttt ctggtactgc tattaaaaat attatctcta ctttattatt
    68281 tgggtctacg ctttcccaat tttctattgg tgtctctaag gtcatagctg caaatgcagc
    68341 ttctttaact gacttagatt ttccatgagg aattgcaagc cctctttcaa aacctgttgg
    68401 agataaagtt tctctgtaca ttacagcttc taaatattcc tcataagatg ttaacttgcc
    68461 ttcatcatat agctttcttg ctaagaactt tattgcgtct ttctttgttt ttattttttg
    68521 ttttaaaaaa acaaggtttt catttgtgat tttcttaaat tccaaaatta cgccctcctt
    68581 aaatcattta atgctatgaa tatagcataa aataaattat gaaaatgttt ttttgaaatt
    68641 atacacacaa tttcttctta aaaaataaag atttgtgtat ttatacacaa atctctatct
    68701 taaccagtat tgctctatta ttgctctcat aatcatcata attggaacct tatctgatat
    68761 attgtaccca tttacttgtt taattgttga atagcaatat aaacttatat ctgcaatttg
    68821 ttgcaattga ttttcacata tatgtgttag agctactacc ttacaaccct tattaactgc
    68881 tgtctttgct acttctataa cttctttcgt ttctccactt aaactaagtg caaaaaacat
    68941 atcttgttca cctaattttc ctatatcatg aagtacttca tgtctttgac tatagtaagt
    69001 tatattatca tgcccacctc taatttcttt tactaatatc tcacataaag gaatgttttg
    69061 acctattcca taaactataa tactcctaga tttttgaatt atatctgcta ctttatttat
    69121 tttttcataa tctattagct ctaatgtctt ttttatatct gattcaaaac cattttttgg
    69181 ctctttatcc tcatttttta actcttcttt taaattattt tttagttgag agaaaccatc
    69241 atactccatt tttttacata gcctaactat ggtatttggg acagtaaaaa atttatctgc
    69301 taaagattgt attgataatt ttactatttc ttctttgttt tttctcatat attcaattat
    69361 catgtcttct gtatctgtaa atttatactc atattttttt gctctttcat aaaattccat
    69421 agtttctcct ccatctattt tattattcat aagtaaaatt caaaaatatt agctaaaatt
    69481 ttcgttaata taattataat ttaatatttt taatttatta atgcatacta ctctttttat
    69541 tatacataaa aaatgtgtac ttcctatatt gatagagatg attcaagttt ttattttgat
    69601 acatctctat caatattttt ttagaaaata cacatttttt tcaaatactt atttttaatt
    69661 aaaactttag gtaatctatt ctttatatat aattattaga cttgaaaata tctttaaaat
    69721 caaccaattc aatattattt ctttttaacc acattttaac ttccttatca catagcattg
    69781 catgttcata tactctctct tgaataagag aagatgattt tataagacta taatctataa
    69841 atcctggatg agtaaccatc aatgttatat ctttatctaa atttatatat ttttcttcaa
    69901 ataatttaac aaaagattta tttaacttgt aaaaatcatt ctgaggatat ttacttggca
    69961 tgctaataca tgaatccaaa taatagggac aacttggtat accgtattct tttgcagcat
    70021 cacacattgc tgatattgat acatcttctt ctataccatg tatatcaata tactcaggca
    70081 atttactcat atacaacttg taagtttcaa tttgagcaca aacttcttca tatacttctt
    70141 cataattaaa aacctcttta cctgtcagta gttgcgttct tcttattttg ctagaaacaa
    70201 attttccacc ttcatctaac aaattcttta tcttaatatg ttctttgcaa caaggttctc
    70261 ctattactaa attaacatgt aaaccaaaac atacatcatc attttttacc atttctatag
    70321 cttgtcttga aaaaggcata ttcaccatga ttccagcact tgtaacaata ccgttcttat
    70381 aagaagatat tatccctagt gtaacacctt ctgaatatcc tatatcatct gccctaacta
    70441 ctagtttctt cattttatgc gtctgcctgc tttctcaact catcaagttg catttccatc
    70501 tcatccttta ctttaacaac atccgttcct atgatgattt gaactgatgt attactcaat
    70561 ttaattaccc ctttggctcc tgttttttta atattatcat cattcaaaat agtagcatcc
    70621 ttcacttcta aacgtaatct cgtcatacaa ttattaagag tcactatatt ttcataacca
    70681 ccacaatttt taattatctg tttagccata tatgaaaaat taccatgaga aagttttaaa
    70741 ttcttttctt tttcactaac tttatcacct atctctatct caacttcacg tcctggtgtt
    70801 tgcacattat cttttattat tatatattta aatgttacat aatataatac aaagaataca
    70861 actcctacag gtataactaa ccacgcttta tctccataac taaaacttaa gacatagtca
    70921 attatacttg ctccattaaa tgaacccaag cgaacaccca gtaaatatag tacaactcca
    70981 cctagaccag aataaaaaga atgaattata tataactttg gagatgcaaa taaaaacgaa
    71041 aattctattg gttcagttac tgttgccata aaagaagtca atgctccagc ggtcattgca
    71101 ccttttactt cttgcttctt tttatcttgt gctgttttat atatagcata agaaatagca
    71161 ggtattgaaa acatcataat tatgtaaaat cctgtccaga agaatcctgc atttgggtct
    71221 cctgccataa atctaggaat ttctcctctt ataacttctc ctgttgatgt tgtatattca
    71281 cccatttcaa ataatatgta agcatttaaa acttgatgta acccagttgg tattagtgca
    71341 cggttacaga ataaaaacaa gaatattcct attgcaccag ctcctcccaa ccaaaaacca
    71401 aattgattca ttgcagcttg tggatatatc cataataaac taaatatata tgataaaatt
    71461 aaagcaacca ctggtgatag tgtaattaca catttttctc ctgcaaagaa gcttaaaaat
    71521 gctggaactt taactttata tgttttattg taagtccatg ctatacaagc tccaacaatt
    71581 atcccaccaa atacacctgc atttaatgta tcattcaata actctaaatt tgacttaaat
    71641 atcatatatg caactacggc tgcaaccatt acacttgtct tgtctttaga ctttgagtag
    71701 gcactagctg ctcctaataa aaataagtag tccacatttg aaaatactat attagatata
    71761 ttagatatag ctggcacatt gagcatattt ggaccaaata ataatcctaa tatacctgct
    71821 gctggcatta cagctattgg cactagcata gtctttccta aaagtgacag ttgttgcatc
    71881 attttgttta agaacctctt catatccaat cccctttcta tttcggttta attttttgtg
    71941 aaaacgattt attcacattt tatattttaa atattatatt tgtttaaaca aattgtcaat
    72001 attttttatt cattttattt ttatcattta gtttaacata taaatccttt taagtgtatt
    72061 gctatatcta gtctattttt ttaattcatt ttttttattt taaaaatatg tttaaccatg
    72121 tatttgaaac aacccacaaa ataattctat tttgtccttt ttatggttga tatgattcta
    72181 ttttcactat acatgtctaa acacatttga tataatttgt tcatatcagt tcaataatat
    72241 tactaaagac ctttaaggag ggataggggt atatggctaa aaataaattt tcagagatat
    72301 atgaagtatt gaaagaggaa atacttgatg gtaaatatac aagtaatatg atgctgccta
    72361 ctgaacttca acttattgaa aggttttcat gtagcagaaa cactgtacgt agagcaattt
    72421 ctcaattaaa cacagaagga tatgttcaaa gtataaaagg caagggtgtt gtagtgttag
    72481 aaaatagttg ttctaatgat ttttttctaa atatgcacaa tttcaaaggt gtagaatcga
    72541 ttgtagaaga taaaaaggtt aatactgcta ctagtgttct ccacttcagc aaaatcttaa
    72601 ttgataataa gttatctaaa aagacaggtt ttaaagttgg tagtgaagta tattaccttc
    72661 acagactaag atatattgat aacataccta aaattctcga cattaactat tttctatgta
    72721 gtatagttaa agaccttgat gtttctatag cacaaggctc tatttataaa tatatagaag
    72781 agtgtctagg tacaaaaatt gtttcatcca gaaagatatt taaaattgaa aaagctacag
    72841 agcttgagtt aaaaacgcta cctctaaatg attataattg tgttggtgtt attaaaaata
    72901 gtgtatatac tgatgatgga aagctttttg aatatactga atcaaagcat acacctgaaa
    72961 catttgtatt catggatgtt actcaaagat attaaaaaaa ttatgtttaa ttaaacataa
    73021 tttttttacg tttagacact ttatcccata ctaacctcac tattctatat acaattatgt
    73081 gtttatacaa ataattattt attgtatatt ttagctttaa tattagttta atgcttgaca
    73141 atttgtttaa acatattata attaacttat aaattacagg aggtatcaat tatgaaaaat
    73201 tggtggaaaa aagcaactgt ctatcaaata tatccaaaaa gtttcaaaga tagcaacaat
    73261 gatggtatag gtgatataaa tggtattata gaaaaattag attatctgta ttatttaggt
    73321 gttgatttat tatggttgac tcctatgtac gtttctcctc aaagagataa tggatatgat
    73381 atagaagatt attataatat agacccaaaa tatgggacaa tgagtgactt tgaaaaactc
    73441 ttaaaagaag ctcataaaag agacataaaa ataatgatgg atatggtcct aaaccataca
    73501 tctactgaac ataaatggtt taaagaatct aaaaaaagca aagacaatcc atatcgtgat
    73561 tattatttct ggaaagatgc taaacctgat ggttctgttc ctaataactg gatatccaga
    73621 tttagtggaa ctgcttggaa atatgatgaa accactaacc aatattattt acatttattt
    73681 gaagaaactc aagctgattt aaattgggaa aatgaaaagg ttcgtgaaga atgttacaaa
    73741 atactagaat tttgggctga taaaggtatt gatggttttc gtttagatgt agtcaaccta
    73801 ctctccaaaa cacctggatt acctgatgac cctataacag gaccaaaagg agatggaaga
    73861 actcactacg ctgatggtcc tcgtatacac gaatatttac ataatatgaa tcaaaaagtt
    73921 ttcaaaccta aaaatatagt tactgtaggt gaaatgtcat ctactacacc tgaagagtgt
    73981 atcaactata ctagagaaaa tcgagaagaa ctatctatgg tttttagctt ccatcatatg
    74041 aaaacagatt ataaaggagg cgcaaaatgg actaatgaaa tgttctctct ggataaatta
    74101 aaaaaagcac aatctgactt tcaatataaa atgtatgaag gaaaaggatg gaatgctcta
    74161 ttttactcta atcacgacca acctagagct ttatctagat ttggagatga tagatttttt
    74221 cgtcaagaaa gtgctaagat gttagccata acactatttg gtctacaagg tactacatac
    74281 atatatcaag gtgaagaaat aggtatgaca aatgcctact ttactaaaat agatgaatat
    74341 gatgatgctg aaagtaagaa tgcatactat catatgatta aatctggtat tgatgaaaag
    74401 gaggcattgc ttatacttca acaaaaatcc agagataatg cccgtactcc tatgcaatgg
    74461 gataatacta tttataaggg gttttctaat cataaaccgt ggttaaaagt taataatgac
    74521 aatattagtg tagaaaatga gcttaatgat tctcgttcag tattttatac ttatcaaaga
    74581 ttaatcaaat atcgtaaaca atatgatatt tttacagaag gtacttatag attgttagat
    74641 gaaaatcata aaaatctatt tgtttatgaa agagagtata aaaaccaaaa tcttcttgtt
    74701 ataagtaatt tcactcatga taatgtagta tataaattac ctgaaactta tttgaataaa
    74761 ttaaactaca caatacttga aaccaattac actagagata taattgaaaa taaaatggaa
    74821 ataaaacctt acgaaagtat aatgatatat tataaataaa gttatttatg taaaaatagg
    74881 tataacaccc tggtgttata ccaattttac taaattcaaa gtaatgttta tagaatatct
    74941 atagattaca ataattattt gacttctaca tcattatatt tctttgaata tactttagtc
    75001 atatagaaat atataggaac tacaattatc atcccaccta atccccaaac tatcttcata
    75061 ggttcatcaa tacctgcttt tatcagtaat aatatactta taataactgc aaatattggt
    75121 ataattgctc ctccaggtat tctaaaattt acatctttac cagcatactt ctttctcaat
    75181 attagtacag ctaaacatgt aggaatatat tgaataaatc tagatacaac acttatttga
    75241 gataatgttg tgaatgaacc cgaccatgct attagtaaag ttcctatgac agatactata
    75301 attgctatat aaggagctcc atttttatta gtttgtctta tagacttagg cattaatcct
    75361 tgttcaacca aagcagcagc agaccttggt gtaaatatgg atgatgcaat atttatacct
    75421 ccaacagata ctatagttcc tattgtaatt atatacccac ctataggacc taacatctta
    75481 atggcagcat ctgcaacagg agtagctgac cctgctaagc cattacccaa cacccctata
    75541 cttattccca gtataagcat atatattact gatactacaa acattaccat taccaatgct
    75601 ttaggtacat ttttcttagg gttctccata tcttttgctg caacagctaa agattcaaat
    75661 cctgtaaaag cataaaataa agttatagat actgctacaa aagctgatga cgcactttga
    75721 cctttttgta ttattacaaa tggggtaaaa ttagaaccct ttataaagaa taatcctaca
    75781 gctataaata aaactattgg taataatttt cctatagtca tgatattatt taagtgctta
    75841 gattgtttca ttcccataaa atttataatc ccaagcccta caactaatat accaattatt
    75901 atatttttaa taagtggttc tgctgcttgt ggccatacag atgatagtgt tacagcaaag
    75961 aaatttgcca tagtagccca agcaatcata ctaactatcc acttcataaa tccaacttca
    76021 aatccaacaa aatctccaaa tgcctctttt gcataaagat agggccctcc atcagtatca
    76081 aaccaactac ctgcttcagc aaaacataaa gcaatagaaa cagctaaaag catacaaaaa
    76141 actaatacta atatacttgc tggacctata tctgtataag catcaccagg taacccgaat
    76201 atacctgacc ctattattgc attgattcct aatagtacaa tactccaaaa acctaactta
    76261 atgccttttt ccattgttat tccccccaaa gccaacttaa tatatttgta ttttttattt
    76321 agatgcttct ttaactattg ccataaatat ttttttcata ttatcataat ctctagtcat
    76381 catttctgga tgccattgta tccctattac aaactcactt ccttcctttt ctatagcttc
    76441 aattaaaccg tctttagatg tagctgaaac aatatatccc ggtgctactt tgttgacaac
    76501 taagtgatga aaactattaa ctaaaacttc tttttctcct agtatttcat ataattttgt
    76561 cccctccttt atctgtactg tatgtgtagg tacattagat agatgttgct gattgtgttt
    76621 aatataagca ccttcaatta aagataaatc ttgatacaaa ctacctcctt cagttacatt
    76681 tataatttgt tctcctctac atatacctaa aactggtttt ttcatatcta aagcatgttt
    76741 taataatttc atatcaaaac tatctctctt tggtgatatt gcccccaact tattatgagg
    76801 ttcttctttc catatgagtg gatttacatc ttgtccccca gatagtataa gcgcatctat
    76861 attagatacc tgctccttta taatctcatc atcatataca atcggtacaa tatatggtat
    76921 agctttacac ataactactg attgtatata atcattattt acataagccc tttcatagcc
    76981 aggaaacatt cctccttcat caattagtaa attacctgat attcctatta ttggatattt
    77041 ctttttagcc aatttcttca ccccttaact tatttgtgaa caataaaaaa tgctacttct
    77101 cttaaagagc aagtagcata ccaagatttt atttatttga acattataat ttattttaga
    77161 aatacttttt ttgaatacga gtaatataaa taactttatt tacttatatt ttattttaat
    77221 cctgtttagt ttgacttttt taaattgttt taagtacaac taataggtaa aataggcatt
    77281 tagattttcc tttttatcaa cctattaaat actgtttaat atattttttc cccttctatt
    77341 taattcactt ccctttctac cagattcaca tattatcaaa tccagctctt ttaacatatt
    77401 aagtattcct cttacctcac cttctgttat attgtagcct aattcattta ttgaattaac
    77461 aataccattt ctacctatat ttttattaag attattggca ttttttaaac actccattac
    77521 taactttact ttctctatag atgttctatc ctttaatatg cttatttctt tactaaaatc
    77581 ttcagttata tttaataagt tatgaggtaa atcatttatt aaaataacat cctcagaact
    77641 cataatgtta atatacatag cagtattttt taattctctt atattacctg gccattcata
    77701 tgcattaata atgtcttgaa catcttgact aattgctctc ttccttttca taaaatgctt
    77761 taacataact tctatatctt cttttcgctc tcttaaaggt ggtatattta tgggcaatac
    77821 atttatccta tagtataaat ctgctctaaa tctatctcct tttatcattt ctaataaatt
    77881 tttatttgta gccgcaatta ctctaacatc tatatctatt acattttctc ctccaaccct
    77941 cattacttga ttctcttgta atactctaag taatttgctt tgtaagtata taggcatgtc
    78001 tcctatttca tccaaaaata tagttccatt gttagcctgt tcaaataatc cttttttacc
    78061 tccttttaaa gctcctgtaa aagcaccttc ttcatatcca aaaagttcac tttcaagtaa
    78121 attttctggc atagctgcac agtttattgc tataaatggc tgactatgtc ttggtgatgc
    78181 attatgaata gactgagcca tgagttcttt tcctgttcca ctttctcccg ttataagaat
    78241 tgtataatca gattcagcta ttttcatacc taatttctta gttctttcca tcttcgttga
    78301 aactgttttt atatcatcaa aagtatatct agcaacttgt cccttttctc taagcttctt
    78361 tgataggttt tgctccaatt ttcttatgta agttatttct tgaatacaaa aataatatcc
    78421 agtaaccctt ccaaaactct ctatattata cttatttacg tttaagtact tattattaaa
    78481 ttttataact tcatcatgtg ccttactcaa actaaaaatc tttttaatat tctcattctc
    78541 taattcatct atatttttcc ctataatatt actttgaata tctagtatat cccttaaact
    78601 attattacaa ataattatgt ttcccttatc tgataccatt gccataccta agtttgataa
    78661 atttattatg gaatttagtt catctgcttt tatagtaagt tctttgtatt tagtatttat
    78721 tcctgaatat aaactaatta tttcatctgt atacttaatt agcctctcag taatttcttt
    78781 atcatccagt ttgagttttg ctattatttg aataaacgtt gaactatcaa tatacctatg
    78841 gcctaaatca ataacttctt ctatatattt tggaactaaa cttgactctc ctggcgttat
    78901 agctatctta atatttctat attcttcatt gggattgtat ggtataaaat tcaaatgatt
    78961 tattcctata ttgtataaat tagatattgt ttccaataca gtatgcttat tatcgtttac
    79021 tactaataca tctattcctt ctggcaatga aaataactta tatatatcct tttgctttat
    79081 gctcctattt atagttatta tctttgagac atcttccaca tattttttta tttttaatac
    79141 tctatcatct atcattacca atattatgtc atcttttatt aattccttct ctgttatttt
    79201 atttaaataa taatttttta tcattacttt atctttaaag atcatttcta gatttttatt
    79261 taagaaacat tctaatttag acaacccatc agttactact gctattgttt tcatttgatt
    79321 actcctttta aaatatactc tactctttta taatatacat ttgattttca ctttaataac
    79381 acaaaaaatt gtacatttag tatactaaca tattccttta ttagttttat gtccataaat
    79441 atcatagttg caatatctat catatttctt aaacttctat aaagcaaaaa ttgtgccaaa
    79501 ttaataattt tcttaatttt attctaaagt tcctcaatag tatttacaga cttagcactt
    79561 atagcttata agtctttata ttattaatct atttttatag gttaattgta caaatcatat
    79621 atcactttta cctattaaat gcattataaa aaattgtatt tataaacact atttactact
    79681 cttctaagta caaacagtat ctacatacta taacctttca ccattagatt ctattacttg
    79741 tttataccag tcaaaactct ttttcttaat cctagacaag tctccacttc catcatcata
    79801 cttatccacg tatataaacc catatctctt ggccatctca ccagtagatg cacttactaa
    79861 atctatacat ccccatggag tatatccaaa taaatcaacg ccatcttcta cagcttcctt
    79921 catttgctct atatgtgctt ttaaatactc tatacgatag tcatcattta tcactccatt
    79981 agattctttt ttatcataag ctcctaaacc attttcaact accatcatag gtattctata
    80041 tctatcataa atttcattta gtgcatatct aagcccttca gggtctattt gccatcccca
    80101 ttcactagac ttaagatatg gatttttcac tccacctaat atgttgccac ttgtattatc
    80161 ttctttagaa tcactactta cacatgtaga catataataa ctaaatgtat aaaaatctac
    80221 acaaccttct tttaaaattt ctaaatctcc tttttccatt tttatttcaa tattattttc
    80281 tccaaaatat ctattcatat aagttggata ttcgccacgc acttgtacat ctccacagaa
    80341 ataattcatt actctactat ctctttgagt tttaactgca tcatctggat tacaagtata
    80401 aggatacatt gttgcaaata tttgcataca tccaacttga taatttgaat ctatttcatg
    80461 agctaactta acagccttag cacttgctac aaattgatga tgtaaccctt gaaaacgaag
    80521 ttgaggtata tctacttggt ttataaaatc tgtagtccct tcatttaata ttccttgtga
    80581 taagtagcct cccataggca ttgtagctga atttatttca ttaaatgtca gccaatattt
    80641 tacttttcct ttatatcttt taaatattgt ttcacagtaa ttcatataca agtctattac
    80701 atctcttgaa acccatccat tgtatttctc tgttattgca aatggtattt catagtgaga
    80761 tattgttatt aatggttcta tattatattt taaacattca tctatcacat tttcataaaa
    80821 tttaagtcct gcttcatttg gttctttctc cattccagtt ggaaatattc tagtccatgc
    80881 tattgaaaat ctaaatacct taaatcccat ttctgcaaat aaagctatat cttctttgta
    80941 tctgtgataa aaatctattg cttcatgact tggataaaat gttccttttt ctataactct
    81001 cgttattctt ttacttactt tttgagaacc tccagtacac atatctgaac aagaaggacc
    81061 tttcccatct aaattccatg ctccttcaca ttgatttgct gctgttgctc caccccataa
    81121 aaaatctttt ctaagtgcca taattctacc tccaataatt tatttatagt aactttattt
    81181 taaaataaaa atacatatat ataattctta attaatagaa ctctctttta cgctaaaaca
    81241 gttatcaatt tctgactatt atttacatgc ttttgtgtat tttctacaat atccacataa
    81301 tcagaataat tagtaacaac tactggtgtt tctaagcaat atccttcttt tgaaatactt
    81361 tctatgtcaa aagttattaa tgtctgacct tttttaatct tatccccttg attaacaaaa
    81421 gatttgaaat atttaccttc caactgaata gtatttaacc ctatatgtat caatagttca
    81481 caaccattat cagatataat tcctattgca tgttttgttg gaaataaagt cattacagtc
    81541 ccatcaaatg gtgccactac ttttccttct ttaggtgtta ttgctacacc ttttcccaat
    81601 atacctgttg aaaacgctgc atcttgtatc tgctccaaag cctttacttc accttttatt
    81661 gggctatata ctacttctct tttgtttttt tctataacgc cttgcccatt atcatcttta
    81721 gaaatttcta ctatagcatc atctttccaa aaaaataatg ttaaaacaaa tcctactaca
    81781 atagctacta ttattgctat tatagttgca taaacacctt tcatatctcc agttgttgga
    81841 tttatataat taggtatacc aaatattcca agtccaccca ttacataact ttttacattc
    81901 attatcccca ttacagctcc tgccacagca ccacctacac aactatatat gaatggtgtt
    81961 tttcttggta aagttattcc atatattgca ggctctgtta caccacatat ccctgaaata
    82021 actgcaggta tacacagctc ttttatcttc ttatctttta atttaaagta cattgctgct
    82081 actactgctg tttgtgcaaa agatggtaca aatactccca ctaatattgt gtcaaacccc
    82141 atagtcataa ggtttgacat agctattggt actaatcccc aatgtagacc aaatattaca
    82201 agcacttgcc acaataaacc agttaatgca ccacataaaa ttggtgaaaa gttaaataaa
    82261 gctaaaaatc catttccaac tatattagaa gcataggtag ctataggccc tataaccata
    82321 aatcccaatg gtaaagaaac taataaaact gccattggta caaagaaatt ctttactaca
    82381 tctggtatta ttttagcaaa cactttctcc aacttagctg ctaataatac tattaaaatt
    82441 actggtacta ctgttgaagt gtagttcatt gctattactg gtaatcctaa gaaagttata
    82501 tatactggtg attcaaatat tgttccagta aatagattgt ataaaggttg acctgtagat
    82561 gctagagtat ctaattgaat acctgaatag cataaaactg cacctataag cattccaata
    82621 aatggtttta atttgaattt cttagcagct gtatatccaa gaattattgg catatactgg
    82681 aacattgcat ctccaattgc atttaacata atatatgcac catcagtttc tccatacaaa
    82741 cccaatgctg taaatactgc attaaatcct tttatcatac cagcagcaca cagaactcct
    82801 aatattggtt gaaaaactcc tgatattatg tctataaact tattaagtaa tccttttggc
    82861 gcatctgagt ctgaattatc tattgtatct cctcctaatc cagccaaatc gcacacaact
    82921 tcataaacat ctggaacatg attaccaata actacttggt attgacctgc acttttcatt
    82981 acagtaacta taccatccat tttttttagc acatcatcat ttgctattga ctcatctttt
    83041 aatttaaaac gtagcctcgt tatacagtga gataaactgc taatattatc ctttcctcct
    83101 acattcgcaa tgatttcttt tgatagttgt tcatactttc tcattttttc tcctccattt
    83161 tctttttgaa atatgaaggt ttggccaact taatgtcacc acccaatata tataaaccta
    83221 attattagga tttatatccg acttaatact tttacttacc actctttcaa cgtgaatagt
    83281 taagtataat ttttcctcat ctgttaaatc gtagttgtat tttttatgta taaattcatc
    83341 caacttttct atgaatttat aagcattttt atatttaact tttattacat ttaataaatc
    83401 atcttcatta ttttcatgta aattattact aacaattcgt tctgcaaaat actttaagtg
    83461 agtaataaat ctatgataat aaactgaatc ttcatcaaat actattctgt aatggtattt
    83521 aaccatattt gaaatttctt gtattatttg tgtaatctca tatatagttt ttatactttg
    83581 atccatttgt gcattaacta tatgcagtgc tataaatcca gcttcatctt ctgatagtct
    83641 aatttcaaat tttttttcta tataatccaa tgcctctaac cctattttaa attcactctt
    83701 ataaaatctt tttatatccc aaagtaatac attttttaca cttactcctt cacgagctct
    83761 ttctattgca gtatacatat ggtctgttaa agaaatataa atactatcat ttaatctttt
    83821 ccctagtttt tctttagcat ataaaattat ttcatctgat aattccatat atcttatagg
    83881 aatatctgct attagttctt taaatttatc agatatattt gggtctgaaa gtttgaatat
    83941 cttatcaatt ttatcctttg atatatgctc tcctgtacgt tttttataag ctaaacctct
    84001 ccccataact actatttcct tatgattttc atcagttgta ataattacat tattattgag
    84061 tattttttca ataatcattg tgatgccccc tttattagca ttaagttaaa ataaatcttt
    84121 tttaattatt tagaatattt aaaatgtaat ttattacata aaaaaaaacc tatttattac
    84181 acattaaata gactcccatg agtcctgagt aataaatagg tttagcttgt atttaaacaa
    84241 tcactatccg tttgtatttt attttaatta ttatttatta tatgttagca aatttatttt
    84301 ttagtgtcaa ctttttctca atattttaat tttaaataaa ttatataaat tgaatcttca
    84361 aagtactatt tttatcaata aacaaaataa aaaaggtatt actttcttta agtaataccc
    84421 atttatttct aatttaagtg gtttttctta atctcatcaa gtagtccatc ttctaaataa
    84481 atttctaatg cacatcctac cattgccttt actgctttgt gaagtttatc ataagcttcc
    84541 tcagaatttg cataatttag aaattctttt tcatgaacat ataccattgg acttatatct
    84601 aaggctattt ctgtatacat agtagggcaa acttgactta cattacctat atctgtagaa
    84661 cctactggac catctttacc ttctaatata tctgttatac ctgcctcaat caaattattc
    84721 tttgttatct tttgaagtac tttgttattt attatgttgt caaaagaatt ttcaaagtat
    84781 ctataagaaa gcttagctcc tgtcattaac tctgcacctt ttgcacagtt tataactttt
    84841 ttagttactt catctaaata acttctttca gcagctctta cgaaaaactt acaagctgct
    84901 ttttctggaa ctatatttgg agcctctcct ccatttgaaa ctataccatg tattctaaca
    84961 tctgatttta catgttgtct tagtgcattt atcccagcaa aagtaagttg tactgcatct
    85021 agtgcattga ctcccatatg agggtgacta gctgcatgtg ctgatacccc ttcaaaacta
    85081 aactctaatg aatctatcgc caaagcctta cagtttatat tattaaaagc ttctatatgc
    85141 atttgataac ttacatcaac atcatcaaaa gctcctgcct taactaaatc acattttcct
    85201 cctgttgttt cttcagctgg tgttcctatt agtacaattt ttcctttaaa attttctttt
    85261 aacttactta atactataca agctcctagt gtagatgctg caatccagtt atgtccacaa
    85321 gcatgagctg gttctttatt ttctccatat ccaggaagtg catcatactc agctaaaaat
    85381 gctatggtag gtccatcatt atctcctaat tcagctctaa aagcagtctc catattacaa
    85441 tatggataaa ctgtatcaaa tccatattcc ttcatttttt ctactaagta ttttgatgat
    85501 atgtattctt cttcccctaa ttcaggattg ttaaatataa aattacaaac ttcttcaatt
    85561 tcttttccaa gttcattctc tatctcataa gctctcttct ttatactctc tatcataatt
    85621 tacctcctac tagaatccta gatataatcc tattgtcaaa aatgccatta caagaattgt
    85681 taatactacc attaaaggaa ttacgaatct aacccacttt gaccaaggaa tattggctac
    85741 tgctagtgct cccattaaaa cacctgatgt aggtgttatc aagttagtta cacctatacc
    85801 aaattgataa gctgtaacta tcatttgtct tgatacatct actaagtcgc caagtggtgc
    85861 aagtacaggt attgtaagtg atgcaagccc tgatgctgat ggtattaaaa atgctattaa
    85921 tccttctatt aagaaagtta aatttataaa tatagttttt ggtaatccat ttaataaatt
    85981 tgctgcagaa tttaaaattg tatctattat aaatccattt tctgctatta ttacaattcc
    86041 acgtgcaaat gctatacaca atcctgctgt cattaaatct cctgctccac ttataaatga
    86101 attaactata tcatcttgtt taagacctga tgctatacct gatattatac ctatagctgt
    86161 aaatatcatt gcaatttcag ttatatacca tcccaacttt aaaactccat atatcataat
    86221 cgccatacct attgtgaaaa ttgctattac catggcttct ctttttgtaa attcctttat
    86281 atttgtatca ctattaacca ttgattcttg atttagtgat atatcgtgta ctaaagattt
    86341 tttagggtct tttttaactt tattggcata catcataaca aatcctatag atatagccat
    86401 atatattata aacataacaa ctctaaactc tattccagaa cctggtgcta tttgtgataa
    86461 tgcttgtgct attcctacag agaaaggatt cgtagtagct gctccaaatc cagctgttgc
    86521 tcctataaat actgtggcca ctgctgtcaa tgaatcatat cccatactgg tcataagtgg
    86581 aataaatatc atataaaaag gtagtgtctc ttcactcatt ccaaacgtcg tcccacctag
    86641 tccaaataga atcattgaaa tcggtattat taaaaattga aacttcttca ttttatttac
    86701 agtatgagct ataacagctt ctatagctcc tgttttatta actatcccaa aagctcctcc
    86761 aactattaaa acaaatccaa ctacttcagc agcatctata aacccgttta taggtgctgt
    86821 aaatacatca gtaattcctt gtggatttga tggtgctttt tcataagttc cagctacaac
    86881 aactgacctt ccgtctatat ctactctttc aaaatttcca cttggcacta tccaagtaag
    86941 aattgccatt aacactatta gcaaaaatat tattgtaagt gtctgtggca tagtaaactt
    87001 tttctttttt aaactgctca tttttaatct ccccactttt taaattaaat attttattat
    87061 tgcatccctt tatcctattt tgtatagatg taaaaaagct caacatggat acatgttgag
    87121 ctaaatgtgc taaatacaac atacctgcat cttcccttta tcttgtttaa gatagctctc
    87181 catcaaaatt cgggataatt ttgatgacag tactgtacct attaaataca gtcccagcac
    87241 tagcttcgga cactagcact tcggcactta ctccttttat attgcctcat tggtttccaa
    87301 cctcttcaat attaatctta gtaaatgcaa cctctacctc aatctgtttg ttagatgagg
    87361 taattcttat taatttttat taattcaaag taattgtagc ataatatgtc gagattgtaa
    87421 atattttcag tataataatt cttactttat gatttcattc catttttcac aaataatttt
    87481 ttctccatat aatttaaata ctgtagtata ttttaaatac acacaaactt tccacaaatt
    87541 ttaaattatt ataatacctt tataataaaa aataaaagct atgtaaactt atcaatttac
    87601 acagctttat atacattaat ctactccaag tacagcttta agctttgatg ctattgtaat
    87661 aactcgtgga ccaaatataa cttgaactga gtttttcttt ttaacaactc cacttgcacc
    87721 caattcattt acccagaagc catcttcata gaatccatca attgaattta gctctaccct
    87781 aagtctagtt gcacagtttg taacattttt tatattatta cttcctccta aaccttctat
    87841 tatagaatgt acaagctttg tatctttatc atctacttcc tcttctactg cattgttgta
    87901 tgctgtttta ccagcatcta cagattgtct ttctctatat tctttcttac tataaagctt
    87961 aacttcttct gaacttcttc ctggtgtttt tatatcaaac ttcttgattg cccatgtaaa
    88021 tattatataa gtaactataa aaaatattgg tatcaaaaat aataatggta acgcatccac
    88081 tttttgtggt tgtaataaat taggaaacat atttctaaat gattctccca ttatagaaac
    88141 attaaacatt tctgtaaata catacgtcaa tcctgccagt ggtacatatg ctacaaaata
    88201 caataatggt gcaacaaata ggaatgtata ctctattggt tccgttaccc ctaccaacat
    88261 ggctgttact attgctggta tcaatatact tattgctttt ttcttattag cattatcagc
    88321 caacttatac atagctaaag ctgctgctgg taatattgca aaatgtatta ataaacgacc
    88381 tgttgtgaag ttacgagtta tataagatag tgtatctggg tctcccattt gagctaatct
    88441 tatattatta acaccctcgt aaattgctcc acctacttcc attgttccac ctactcttgt
    88501 atattctata gggaatgcaa ttaagtgatg tataccaaat ggtaataatg ctttatccat
    88561 tgctccaaaa gtaaatgttc ctaataatcc actagaacct atccaattac caagtttaga
    88621 taatgccaac cctactggtg gccatataaa gtacattgct gtacccaata ctattgatgc
    88681 tataaacatc ataatcattg aaaatcttgg acctgagaaa aaagataatg cactaggaag
    88741 ttctatgtca acaaatttgt tatgtactgc actagctaca aatccagcta ttatagctcc
    88801 aaatatacta caatcatatg aaaagattcc taagaaagaa ccatataaag aacttcgact
    88861 atatgcttct gcctctccta atccagatgc cataaacgct tctgctgtaa ctgtatctga
    88921 atttatacca tttgctgcta acaatgtact tataactgtg tgcattccta taaataaaac
    88981 aattcctcct aatgcacccc atcctttttc ctttttagat aatccaaagg ctatacctgt
    89041 aacaaagaat attggtaagt atctcattac cataaatcct aaatcgttta ttactttaaa
    89101 gaatacattt ataaatgacc cttcttttat tagtccgcta agccctaatg cagaaacgtt
    89161 ttccatattt gcaaaagctg accctagtcc tataaatact cctgctacaa caagtaatat
    89221 aacaggtatc atcattgctc cagaaagtac ttgtaattta tccttcatag tattatttcc
    89281 tccattttta ttgaaatgcc aggctatata atgcatatat atatacttct attgctttgt
    89341 ataatgtatc tatacttata ttttcatttg cctgatgtga atttggatta tccccctcta
    89401 ttgtgggtcc aaatgcaact ccatttttta acactcttgc ataagatgca gctccttttg
    89461 taaaaagttc ggcttctttt ccaaaacctt gtttatatgc atttgataat ttatttatta
    89521 attcactatc tggttttaca tacagtaatt ttaaatcctt atatatatca actattaaat
    89581 tttctttttt tccaatttca ttaattctat tagttataaa ctcccaactt atacccttag
    89641 gatatctaaa gtctattttc atattcaatt catttttctc taagaagata ctcattatac
    89701 atattgtaga aactcccata tctacatctt ctttatatat ccctaatttt tttccatgat
    89761 tatcatctgt aaagtatgaa tttaaaatat cccttattat aacgccttta tcatttagtt
    89821 ttttaatttt ctctaaatct tttgctaaac taaatgcaca gttatatgac ctgtgtggat
    89881 ttctcgataa agccctttct cctgtatatc ttactaatac tttaccttct tctagttctt
    89941 ctatctctgt ataataagct aatgattcta ctaaatcttt cttatcatta gttttaaaaa
    90001 cagcctcaat tttgtcacat acaaatccat cttctttgtt tgactttata tcaactaaat
    90061 tgtgatttct aaatttatct ttatctattt tctttcttag attgaagtat aaaatacctt
    90121 tttctccatt tactattggg aagtttccat ctggagaaaa tgcaaatttt ggctgttcat
    90181 tgacactaaa gtaatgctcc atacattccc aagtcgtctc ttcagctcca cccactatca
    90241 atctaatctt tctcttcggc ttttcattaa gttctcttaa gaaaagtaat gcatataaaa
    90301 tccctatcaa aggtcctttg ttgtcattaa ctcctctacc ataaagatag ttatcttttt
    90361 gacatacttt aaaaggttta ctcttccaaa gttctttatt atatattggc accacatcta
    90421 tatgattaag cacccctact acttcttctc cttctccata ttctatatga attgcatatc
    90481 catcaaagtc ttttacaaca aaatctttgt cttttgctat ttgaataagc ttatcgaagg
    90541 catttcttat ttctatccca aatggttgat ttatatctgt tgtactttca tctctaattg
    90601 aaggtatttc aactaaactt ctaatatcat ttatgatatt ttcttgattg gattttaaat
    90661 aatcaataat tagttctttg tttactatca tcttaattat ttatcacccc tttatattaa
    90721 ttttacaaag catttcagtt catatctaca ttataaattt tgaatattat tttttcaata
    90781 tttcaaatac ttctctaagt taccataatg atacggtaaa caatcaacag attatgacaa
    90841 aatcatacta tacctattac taatatttta acttatctat tacgtctttt ttaatgtaag
    90901 tttattccaa aaatacataa aaaaagatgt agccttagct acaccttctt atatctattc
    90961 aacttccatt attatgctat taatatctac atttccaggt tctatcctat cttttactgt
    91021 tctcttaaca gaatcagcat catccattat aactacagga gttattatat ttaaatcatt
    91081 tgacgttata aaatctaaat ctacttccat tattaaatct ttagctttta ccttatctcc
    91141 tgcctttacc ttaacatcaa acggctttcc ttctagtgac attgtctcta aacctacatg
    91201 tattaatata tttgtattat cattactctt aatcattatt gcatgctttg tatcaaatac
    91261 attgactatt tcaccatcaa caggagaata taacttacct tcacttggta ttattgctac
    91321 tccatctccc atcatctttt gggcaaaaac tgcatctgga acatcttcta agttcactaa
    91381 atttccctta aaagggctat atattttctc aatcttattt tcttttttct taaatatatt
    91441 aaacataaca cactctccac attataaaat tttatgataa tatttttctt aaagcttttg
    91501 ctacaccatc atttacatta gtatcagtta caaaatctgc catagatttt acttcatctg
    91561 tagcatttcc catagcaata ccaagacctg caaatttaat catggatata tcattttcat
    91621 tatcacctat acaaataact tctttgctat ctatacctaa tatatcacaa aattttttaa
    91681 ctgcaatacc ttttgaagta cctttactca taacttcaaa gtttatcttt ccagaactta
    91741 ctacttctaa atcttcttgt tttttaaatt cttcctttgc ttctctcaaa gaatctacgt
    91801 tttctgagaa aagtatacat tttaaaattt cattatcagc tttttctaat aaagtttcac
    91861 aacttgcatt ttctattatt tcaattctgt cttcttcttt atttttacta tttattaatc
    91921 tatatggatg ttcttcacct atttcattgt tggatataac tattttacag cctttaaaat
    91981 gtttgcttaa attatacttt tcacctattg tatagatttt ctttagagct tctttgctca
    92041 aaaccttctt atactcatat cccagtaatt taaaatatgt cccattagtt gatataatat
    92101 atatatcatc ccctaaaaca cttgtaaaat atttcatagc attataaggt cttcccgtaa
    92161 ctaaggctac cttgataccc ttttccatga cttcttttag tgctttaata ttttcttctg
    92221 atacttcaaa tcctttaccc atcaaagtac catccatatc agtacaaatt aatttatatc
    92281 gcatttgtat cacatcctag taagattttt ctaatatttc aacaaggtct tctttctttc
    92341 caaaaggtaa aaatcctaaa actggacagc ttgttatttg ttctaattgt tcctttgtta
    92401 cattaaactc agacattttt gtcttaagtc ccactctctt taaaaaactt tctatttcat
    92461 ttcctaaagt tttagctgct tcatcatctt ccactttttc taattcttgg tttagcattc
    92521 tcgctacttt ggcaaatttc tctatattat ccttatagaa cttttctgca aattttggga
    92581 acactatagc caaagcctca ccatgaggaa tatgtgtaac tcctcctatt atttcactta
    92641 gtggatgtgg tgctgctgct ccagcatttg ccagtgaact tcctcctaat gtatctgcta
    92701 ctgctaactt tgttctgtat tctatattat ttttatcttc cattacttta ggtagatagt
    92761 taatgactaa ttccatagat ttttcagaca ttatctctgt aaaatcatta gcatttggat
    92821 ttatataact ttcaaatgca tgagtaaaag catcaaatgc tgttgatgca gtaattctct
    92881 ctggcagagt taacataagc tctgggtcaa ctatacattc ttttgagaaa caattttgat
    92941 gaaatattgt atttttctcg cttcctctcg atattactgc tgcttgtgta acttggcttc
    93001 ctgttccaga agtagttgat acagctatta atggtaattc tttttcactt accttatcgt
    93061 atattttaaa tggatttgta tatgtactaa acatataatc ccaatctatc ttttctaaac
    93121 caaaagtcaa tgctaatact tttgcagtat ctatacttga acctcctcca acagctaaaa
    93181 caacatctac agattctttt ttagctaatt caaacccttc atttattaat tctacagttg
    93241 ggtttggctc tactctatca aagtgtacaa cttctatacc tttttctatt aaattcaatg
    93301 taactctttt atataacttg tctaatggct ctacatttgg agtagtaact aacaaacatt
    93361 tctttccata ttttcctatt attctaggta attcacttaa agttccttgt ccaaaattta
    93421 ttcttgatgg ttgataaaag ttaatcatat ttttcctccc ttaaattatt gccttcatat
    93481 tcaaattata catatcatag gatttttaag gcaatacctt cactgttaac tcttatttac
    93541 taaagcttat ggcaattatt agacaatcaa tgttttatta ttaccatatc attatgtaaa
    93601 ttttattaag tacccatgtt ataattttat aatcaatctt attgtataat tgacttaata
    93661 ataaaaatag agtatataca cttttttatg acaaatagct taaaggagaa ttaaattatg
    93721 aaatatttga tagacagtgc taatcttgat gaaattcaaa agtgtttaaa gtatggattt
    93781 aagggaataa cagctaatcc atctatgtat cttaaagaga aagtaaactt ccatgaattt
    93841 attgagacat gtgctaattt aaatccagaa atattaactg ctgaagtcat tggaaataat
    93901 ttatacgaat taattaattc atgtgataag atattaaata taaataaaaa cactataata
    93961 aaaataaatt tctctgaagt tggcttagaa cttataaaca tattaaatag taaaggtgta
    94021 aaaactgcat gtaccttgat tttcaatgta aatcaagcat cattagctat aaatgcaggt
    94081 gctgattacc tattcccttt tataggtaga aatgatgaaa atgggaatga tggtataaaa
    94141 aaccttacag aaatatgtaa ccttatatca ataaatggat ataatactaa agttgttgca
    94201 gcttctatta aaaatgtatt ccacttaact caagcatctc tttgtggatg tgattatgca
    94261 gctgtaacat atgatttatt taaaaaagct tcttttaacc agcttacaat agatggtgcc
    94321 aatacttttg aaagagattg gtccaaggtt aataaatagt aactaagatt taggagggtt
    94381 tgcattgaac aaaagattat tagatattgc tgaattatta ctcaacagct cagactatat
    94441 tactgtagat actattgccg aagaattaaa agttagtaat aaaactattc gtaatgacct
    94501 tgttatttta gatgaatggc ttttagagtt taatttatcc ctagataaaa aaactggttc
    94561 aggggttatt attctaggta atgaagatat aaaacttaaa gttattagag atatcaatga
    94621 taaatcaaat tgtatatatg catactctcc tgaagataga aaaaggtata ttctaagtaa
    94681 attatttatt aaaaattcta agtttaggat tcgtgatatt tgcaatgaac tacatgttag
    94741 tagagctact gtgcataaag acttggtagt tattcaaaat tttttgcaaa actttaaagt
    94801 gactctagtt agaaaaacca ataatggagt atatttagag ggaaaagaga aagatattcg
    94861 aaaagcaatt tttgaacttg ctacggttaa taaaagttat actgaactta aagaaatatt
    94921 attctcgaaa tctaatgaat gcaaagatac cccttcaaca aaaatattta aggagctatt
    94981 taactatgat tttgagaaat tatctcaaat cactctacac accttacaag tagaaaaaag
    95041 taaccttagt gatgagtatt atataaactt cttaatacat atagctatat gtattaaaag
    95101 aatttcaatt aaaagatata ttaacttatc tgaaggtttt tttagagaat tattagaaca
    95161 tgaatacttt gaaaaatcaa aaattttatg tgctaaccta agtgaagctt ttgacgtaga
    95221 atttattgat gaagaggtct gttatatcct tcttcatata gaaggattac ttaaatcaat
    95281 aaagaaaact actactcttg aacctcttaa aaatgaagaa attacagagt ataatctatc
    95341 tgaagcaata gctgttcatt ggggaagtat actaaattta aacttaaagg atgatttaat
    95401 attaataaag tctcttgcta atcaccttaa atcagttcta cacagaataa actatgggtt
    95461 tactattaca aatcccatat taaatgatat aaagagaaca tttccttata cttataaggc
    95521 tgctaaagag tctaatactg ttatcaaaca gttgatgaat tatgaagtca atgatgacga
    95581 aataggatat cttgctttat atttaatatc agcaatagat agaagcaaat ctccccttaa
    95641 tactatttta atatgtcact gtagtgatgg tgtatctaat ttattagtcc aaaagttaag
    95701 ttttgaattt aatcagatta atatagttaa atctatacct ctatcttcta tttcatttac
    95761 gaactttgat gatgtggact taatactaac cactgctcct gtagactttg aacataatgc
    95821 tgaactgata aatataaatg ctcttttaag taagaatgat atttctagac ttagtacaat
    95881 tataaaaaaa ctatattctg aaaaaaataa aatattatct tataaataaa caaaaatagg
    95941 cttgtctcaa catagagata aacctatttt tcattgtcta aatgataaaa atatatttga
    96001 tttcatagaa aaagtgcctc atgaactaaa aaattcattt tgagacacct ccatttttta
    96061 tatttaagct ttttatttaa gcttttatat tttttaaact tttatattta aagtaaattt
    96121 tattattttt attgagcaca agcattattt atattattaa tttcttcagt attcttacca
    96181 aactttgtag ttacaaatcc tattgttttt attatgagta ataaagatat caatgaatat
    96241 atagatatca taaaattctt acttattatt ccagacgcta ctattataaa tccatctagc
    96301 ataaataata taactggcaa cttcagaaac ttaattacct tattaataag cattgcaact
    96361 aaatcagttc caccagtact acagcccctt attattgcta tccctatagc agttcctacc
    96421 aataacccac ctatcactcc tgctactaat agattgtcag ttatgtgagt ttgtggtatt
    96481 acttttaaga ataatgggcc cataagagtt atatataatg tttttattcc aaaacctttc
    96541 cctaaaaata ataatcccat tactaaaagt ggcatagata ttcctaatga tatattctca
    96601 acaggcattg ataagaatgt gctaagtacg actgataatc ctgttatccc tcctggagct
    96661 aatgtatgtt ctgcaaagaa aaaattaact gctaatgcca ttaaaaatgc tgatattgtt
    96721 atcagtaaat aatcttttat catttctttt ttcatttaat aacctcctat gattatatta
    96781 tttcagtata aaaaatcaat taaattagtc tagcttcctg tagtttttca taaaattaat
    96841 gcttccttaa aacattaaga aagcatctct tttatcatag tttattatct tattctaaac
    96901 caaactttgt agctgcttta tctatagctt ttctacaatt cttaacacct gctgttatac
    96961 catctttatc cttgaatatt gctgaagata atattacaac gtctggatta cattctatta
    97021 atggctccat attgtctgct cttagtccac catctattgc taattcacac ttaggattct
    97081 tttcgtctat catttttcta gctcttttta ctaaatcaac agcactcttt ctccatcccc
    97141 agttatcttt tccatcagtt tcatcaacac catgaacgac tatttgcaat ctatctatat
    97201 catatatagc ttcatcaaca aaacataatg gtgtgtaaca tccaattgtt aatcctactt
    97261 tcataccaaa ttctctacag taatttatta tgtatgcaag aggagcacct aaaaaatgtt
    97321 ctgctggtat tatcaacaag ttacatcctg ctgctgctat tttttctata aataatctat
    97381 cacaatctct tgtgtatata tggcattcta ttggtttatc tgtcactggt cttattgctt
    97441 ctattatttg atgaccaccc ataagttgca tattttttaa gtcatgcata tctgctgcat
    97501 ctgaatgcac gtagtctaca cctgcatcca ttgcttcttt aactacggct gatatatgac
    97561 cgtaatttac atgtgctaat cctgctgcta tttttatgtg tttcatattt gtatacctcc
    97621 atattttact ttaattagta ttaaatatta gtttttgata tttttttaat attttttaat
    97681 ccagtattac ttttatttag cattactttt attgctgtat atatttcttt tatgaactta
    97741 ttctgctttt gttaactgtg aacttatttt gctttgtaaa ttaatattac actgatatat
    97801 tttttttcac aacactttga ctactcctta aaagtacctt tatattgtgg taaattatag
    97861 acattaattg tatataaaaa gttagttttt taccacattc ttatggcatt tattgtctat
    97921 actaactcaa ttgatatagc tagttcattt aatatataat cagactataa atataaattt
    97981 actcgataaa aagtaccatt cataacaata ttaatgaaag ggagatgtaa aatgagaaac
    98041 tgtattattg cccaatctgg aggccctaca gctgttatca atgcttctgc agttggtatt
    98101 tatgaaaaaa atttagaaac taaatacttt gataaagtat attttggtat caatgggatt
    98161 gaaggtatat taaacaaaaa tataatagat tcagactcct taaacaaaga taatgtatta
    98221 aatttaaagc aaacaccttc gtctggtctt ggttcttgta gatacaaact ggctgattat
    98281 aaaataaatt cttatgatta tgatagagtt ataaatatac tagatgaata tgaaatagat
    98341 acattatttt atataggcgg aaatgattct atggacacag tgatgaaatt ttctcaatac
    98401 tgcaaaaaca ctacttgtaa aataaaattt ataggtattc ctaaaacaat agataatgat
    98461 ttattaggaa ctgacaactg tcctggattt ggttctgctg caaagcttat ttctacacta
    98521 ataatggaaa catacttaga tttttctata tacaaaaata atggtatatt tatagtagaa
    98581 actatgggta gagatactgg atggttagca gcttcttctt cactagctag acttaatgat
    98641 actccacttg ttgatttaat ataccttcca gaaattgatt tctatgaaga taagtttata
    98701 gaagatgtta gaaaaatatt taaagaaaaa aataaggctt atatagttgc gtcagaaggt
    98761 ataagggata aatacggcaa ctttatatgc gaacaaaaaa cttctgctca tgataaattt
    98821 ggtcattccc aattaggtgg agtaagcaac tatctaaaga atcttatagt atcatcatca
    98881 attacaacaa gagtaaaaac cttagaactt ggtgtaatac aaagatgtgc tatgcattta
    98941 gcttcagata ctgatttaaa acaagcagct gaagttggtg catatgggat agaactatct
    99001 aaaaaaggat atacaggagt tatggctatc attaatagaa catgcaataa tccttataag
    99061 tatgaagtta gtcatactga tatttctaaa atagctaaca agacaaaata ttttcctaaa
    99121 gagtggataa atgaagaatg taattatata actgatgaag ccctaaatta tctatatcca
    99181 cttattcaag gtaaaccaga tatcaaatat aaaaatggac taccttattt ttcaattatt
    99241 tactaggaaa ttattataat ttgatataca ctcaattcta tcgctcttaa atacctaagt
    99301 ggctgttaaa taaaattata aattgttata tgacagtcac ttaatcgttt aattatttaa
    99361 ctgtttagtt ttttaatttt ttaatctttt ttaatactaa tacgatttgt tgtaataata
    99421 tctcacaccc attttcattt tggatagatt ataattagtt agacaataaa tattttgtca
    99481 tgatattatt atatatagtg atacaaatag aaatactttt atcatggcta gaaaaacaaa
    99541 gattacacaa aggatttaaa aatcaaacaa cttcaattaa aataaatggt taaaataact
    99601 tctaaatcaa ctactataaa caataaaaga acataaagta gttaagtcta caattataaa
    99661 attagtaaga ctataataac ttaaataaaa ttaaagttaa caaagactat taaagttagc
    99721 aaatattcct ctacctaaat aattgctttt tataaagagt tatttatttt aattgaattt
    99781 aattgaaata cgattttaac tagaacaata gattaatgta atatttaata taaaataatt
    99841 ataataattg aaaaggaagt aatctatgat gaaaatagca gtcataacaa ccgaattttt
    99901 aaaagagttt gtagataatt ctataaaaaa gttaaatatt aatgcagaaa ttgaaatata
    99961 tatctataga gatttttctc atgttggaga tttatattta gaaatagaag ataggtttga
   100021 tggatttgca gttagtggcc ccatccccaa aaaagctatt acaaaaaagg ctggaactat
   100081 taaaaaacct ctagttgatt ttggtactga cttacaaagt tactatgata tgtttctaaa
   100141 gcttatcttt aaatataata cattagattt ttctagagcg tactttgact taacagactg
   100201 ggcagataat ccaaaagata tatcatatta tttatcaaat ggcacatttg gagacttaat
   100261 ggatagtatt gatgaaaatg catctaaaat gagtttatct gacatagcaa aacttgaaaa
   100321 aaagattgca aaaaaacata ttaatctatg gaatgaaggg aaaatcgatt tttctactac
   100381 tcgttttagt agtattgttc ctatgttaca aaatgcagga gtaaactttc atttcatata
   100441 ccctgatata gatttattaa aaaatacttt taataactta ataaaagata ttcatttaca
   100501 tagaatgaag ggaaatcaac caagtgtaat atatataaca gttaaagata caaattatga
   100561 tgactctact ctagaccaag tttataaaat attaaatagt attaaaaaag ataaattaac
   100621 cgattttata attgaaaaaa atagtaattc ttttaaaatt ttcactaaat gtgaaacgat
   100681 taaatcatta actaaaaatt ttactgcatg ttatataaaa gaatctattg aaaatagtac
   100741 aaatattaaa gtatgtgttg gatatggaat tggtaatgat ataaagcaag ctatgtctag
   100801 tgccataagt gctaataagg aatctaaaat aaatcattta aattacagtt tcttagtcaa
   100861 cgaaaattat gagacaatag gacctttact gtgtgataag ttacttactg tttcaaataa
   100921 agtaactcca catataaata caattgccaa aagttctaaa ttgtctactc ttactattca
   100981 aaagattata tctgttactg aaattctaca aagagatgaa gttactccta aggaattatc
   101041 ttcatgttta aatgtaacag ttagaactgc taatagattt ctaagtagtc tcttaaagtc
   101101 tggaaacgct actatattat atgaaaaaca aaactcaaca aaaggtagac ctgaaagagt
   101161 atacaaatta tttatatcag aaaataaata ttaaaatcca aaatgcataa aaatatttta
   101221 tcatctaata ttaagtaaaa ttatatatga ctttctaaaa ggtatatctt tgcatctttt
   101281 taagatatac cttttataat atttaataat attttatgtt attatctact aaaattattt
   101341 tctaaccatt ttactgcaca atgagcaaat aaagctgaac cataaactaa tccctcttca
   101401 ttaaaaattg ctttaggatt atgcatacca aaattatatt cctgactaat acttcccatt
   101461 ccaatccata aaaatactcc tggaaccctt agtaaaatct ctgaaaaatc ttctgaaccc
   101521 attgcagatg gaaagtctat tacattttca tttcctataa cttctttaac ataagataca
   101581 atctcatctc ctacatccaa atcatttata actgcaggag tccccatttt aaattccact
   101641 ttagcatttc ctccaaatga tttagctatt cctgtagata tttcaataat tctatttttt
   101701 aatttttctc taacagattg gtcaaatgtt cttaaagtac ctgacataga tgctttattt
   101761 ggtatagcat ttggtacgct tcctgcatta aacataccaa tagttaaaac tacaggctct
   101821 ttctgaggtt tttctcttga aagtatagtg tttaatgcta tttgtatatg tgacattatg
   101881 tttattgggt ccactccaaa ttcagggctt gctccatgag ttgcttttcc ttctactgtt
   101941 atttcaaata tatctgcaga tgccatagct ggaccacgtg aataagctac ttgacctgtg
   102001 ttaagaaatt tacccaccat actgtgaaat gccatagctg catcgacctg aggattttct
   102061 aatataccat catcaatcat tgattttgca cctctggtag tctcttcatc tggttcaaat
   102121 acaaacttta ctctaccttt aagtaagtgc ttattatcca taagtatttt agctgctcct
   102181 aagagcattg ctgtgtgatt atcgtgtcca caactatgag atgcatcttt attcttactt
   102241 ttaaaaggta aatccgtctc ctccataata ggaagtgcat ccatatctgc ccttaataat
   102301 aaggtctttc caccattttt actgtctaaa acagctacta ttccattatt agacgtcaat
   102361 ttcggagaat atcccatttt ttctaaagta ctggtaacaa aggttactgt tttcggtaga
   102421 ttgcaaccaa tttcgggatt ttcatgtaaa tatcttctcc atctaataat ttccccttct
   102481 attgactgcg cttctttaaa aaaatttttc atattccttc ctcctaatca gatagtcagc
   102541 aaaatctcac tttcttttag taatgaaatg atgctgatat aaaaattata attttatata
   102601 ttaaatttca tgtcttttta aggagttgga atatcatctg gaataggaca tttatatgtt
   102661 ttaccattca tagatttatc aaattcttct tttgcctttt tagctatctc tggattattt
   102721 aatattttag ctccaaacat agccataatc tttgatgcat atatcatacc tttttctcct
   102781 atagaacttc ctgcacaaga tgtaaactgc cagctatgac ctggtgctcc tagattcgta
   102841 caccctgtaa aaaagtatgc tgtcggaact atatgcatta catctccaat atcagtagag
   102901 ccataactat taaagtttgt aacatttcct ccaccataat atagatgagt tccttctggc
   102961 aaattatatt tacttatcat agccttatat tgttgtgggc tttgtctatc taattcagct
   103021 gcaaaatcca attcctctcg agtccaaggc tcttgtttaa tctcattcat acattctgat
   103081 acaagatttg ctaaaacatg attgtttaaa gtattataac accctcctaa aaactctact
   103141 tcaacttcag tttcagtcat cattgcagct ccttttgcta cctttacaag tctttcatat
   103201 gtactttcta cagcttctct acttaaagct cttgtataat accatactga tgctttatca
   103261 ggaactatgt tgggtgctac tcctccatct gtaattgtat aatgaatcct tacatcagaa
   103321 gtaacatgtt ctctcaaata attagctccc acattagtaa gttccacagc atctagtgca
   103381 cttcttccat tttgagggtc tgagcctgca tgtgcagtct ttcccttaaa gtgaaacttt
   103441 acagagttaa cagctgttga agtaccaatt gtagcttcac taattttatt tggatgaaaa
   103501 tttatagcta agtctaaccc ttcaaacgct ccacctctag ccataaatcc tttacctgtt
   103561 aattgttctt ctcctggaca tgcataaaat actatagtcc cacttaagtt attttctatc
   103621 atctctcttt taagccctac tactgcccct acatgtgctg tacataataa attatgccca
   103681 catccatgac catatgcacc caattcaaat gcttcttgtt ttgttgaaac tttttgattt
   103741 aaaccaggta atgcatcaaa ttctcccata aaacctataa ctggtttacc tgaaccaaaa
   103801 ctagctctaa tagccgtggc taaacctcct acacctactt ccacatcaaa tccttcactt
   103861 cttagaaagt ttgctgtatt ttcacatgct ttaaattctt taaatgcttt ttctggattt
   103921 tcccatattt tcttacttaa atcatcaagt ttgaatcttt ttttatctat ctcatttata
   103981 gatatacttc ctatcatttt ctattcctcc tataaatcta tttactatca accaaatata
   104041 attggaccta ttatactagc aaatgccact gatgcaatgg ttacagtaac aaatcctcct
   104101 ataatcatct ttggtaataa ataatctgtt gccctttgtt tttcttcttc agttccttcc
   104161 atagcatcaa taccttcatt ggaaattatc tgtgttgcag gataagctag catacaagtt
   104221 acaccaattg cagctgacat tcttggagaa tatcccaata gttttcctac aattgcagta
   104281 aatagtatta taaatacaga gcctacaaca agtattccta tagttggaat aatcatccca
   104341 aacaatgctg ttggagttac cttagcaagt cctccaggta acatcaacat acaaccaaac
   104401 attataattc cattagattg agatttatct aaaattcctt tttctaaaaa acctattcgt
   104461 gtaaaaatca accctactaa caaacatgct atgttgggat ttaaataata gtttgcaggg
   104521 gtaaatcctg gtattaaagt agccaatcct acaaaattac caatcatagc aacaaatgaa
   104581 agctttgcaa gatatactaa agtagatgat tttttaccta aatcttcttt aaaaattctc
   104641 atacttggaa gtttaaaatt actttttgac tcatctttca aaaaatcccc gttatctctt
   104701 ttaaatttta attctttatt tatacaatat gtagttatcg gaatgccaaa gaacttttga
   104761 aatgataata ctagaattac aaatgcagca agttcaggtc tatttgcagc ttctgcaaca
   104821 gttgttacga taataccagc aacagtagaa cccgctattg gaggtgctgc tattaaagca
   104881 tactctctac caaataacat actacccact gttaaacagc caacccctat tcctacaata
   104941 cctactaatg caattacaac cgttttccat tcatgacata agtcttcaag atttataagt
   105001 gttcctaagt tagttattaa taaagctata ccaaagttgc tcattgcagc taatatccct
   105061 gatgctcctg taatgtcttt tggtaaaata tttgtccaaa atcctagtaa aaatataata
   105121 catacaaata acaatgttga aattgcacct tttgttttct tagataaaaa ctcactaaaa
   105181 gcataaatca ttacacatac agtaaagcaa gtaagtggat tccaaaaatt ttccattgtt
   105241 gtttcctcct aaatataata attttttatc aataaaacaa taattataaa atgtatatta
   105301 tgcagttatt cttaactaca tatggtatag cttgatattt tttgatgata ttatatacgt
   105361 cctaaattat tcctaaaata tctaaataaa tataactaga gttaactctc taaatattaa
   105421 ctatagttat caacttacta gatttctttt tgatttttac tttctacata attttacgag
   105481 ttagaattgt acttcaacta ttctctatat tagtatacat gttggaagac ttatatttat
   105541 ttaaatatta gatatagaat atatttttat aaacgtctta aattattctt aaaatatcta
   105601 aaacgaaata ataatatacc cattaaacca atattatcag aatatacttc actttaagtc
   105661 ttctttatat agaaatttta tctattagtg catcccctcc cttgtaaatt agactatttc
   105721 taagcgaatt tagatatctt attcctagag actattacta aaaacaaaaa ttatttatta
   105781 tattaaatca atgataagcc atactttaaa attctttcta tacaagtata atttatcata
   105841 taatgacatc tataccttta tctttatagt agtcctaaat tattcttaaa ttgacaataa
   105901 aaataatttc actatttata aaaatacttg tttttatatt gtcaaactat tcttaatata
   105961 gattataatt ttaaatcttt tctttgtcaa tatattctga catttttcca gaaaatttat
   106021 aaagttatat agtgagtaaa tcatactatt tttatataat gagtaaatta tactatgaat
   106081 atgtctaaat atttacttaa gtataaaaat aaaaaatgtg tattctcctt tttatagtaa
   106141 ttttaattgc cattattttt aagaagaata cacactttac tttaaagacc ctatactcta
   106201 ttttaaatgc tttactagac actaattaac attagttcct aacttctatt tatcttattc
   106261 ctaccctact actatatccg tagaaaaatc taaaacttct ccattagatt ttattacttc
   106321 tttgtaccaa tcaaatgatt ttttcttata tctcttaaaa cttcctttgc cttcatcatc
   106381 taagtcaaca tagataaatc catatctctt actcatctga cctgtagttg cactgactaa
   106441 atcaattggt ccccacatca aatatccaaa acattcaaca ttatcttctt caattgcaga
   106501 cttcatttga gatagatgct tacttaaata ttcaattcta taatcatcat ttatagttcc
   106561 atcttcttcg attttatcat atgcccctaa accattttct gtaattaaga taggtaattg
   106621 atactttctg taaatatagt tcaaggtaaa tcttaaacct atagggtcta ttgcccaccc
   106681 ccacttactt tgttccaaat atggattttg aatcccacca aataaagatt tatcacctga
   106741 taaattttct tttggagtag atacacttga aaaataataa ttaagtccta taaaatctaa
   106801 cttaccttct ttaaatgctt ttttgtcttc atcagtcact tcaatagtta tattattttt
   106861 ttcatattct tttaatttat attgtggaaa cttcccattg cacattgcat caatttgata
   106921 ataatcacgg tcattatcta aaaatgcatt taacacatta ctaggattac aattgtatgg
   106981 ataaattgga tttagtccaa atacacatcc aattttattg ttgggattga tttctctagc
   107041 caactttgca acctttacac ccgccaaagt catattatac cctacatttg ccaatgtttg
   107101 aaccttattt tctatttcac tatacttaat acctgctatc atataagtaa aaatatccga
   107161 gtgctctgtt tgagggtcaa tatggttcat ctcattaaat gtaacccagt actttacctt
   107221 atcatcaaat cttcttatta cagtctcact gtactttaaa tacaaatcta taagttttct
   107281 gttattccat gaaccatatt tatgtactaa attcataggt aattcaaaat gatatagtgt
   107341 tacaataggt tctatattat tttctattaa tgtatctatc agaccttcat agtatttaat
   107401 ccctaattca ttaggatttt catcatctcc atttggaaaa attcttgacc aatctataga
   107461 tattcttaat gcactaaatc ccatttcttt aaataaagct atatcttctt tatatctatt
   107521 ataaaaatct attccattat gagatggata atctaatttt tcttctattt tatcagtaat
   107581 tactcttggc gtttcataag agcccttagt tacaaagtcc ataattgcag gtcctttgtt
   107641 atcactatcc catgaacctt cacattgatg ggctgcaata cttcctcccc agaaaaatgt
   107701 atctttaata ttcatgaatt ttccctccta aaatttatac tattacagtt aataattctt
   107761 ctgattcatc atttgatgag attacatcta aataatcgct agtatttgta actacagtta
   107821 ttatagtaga atcaaagcct tcctctataa ttttatcaga ttcaaatact gctaaagcat
   107881 ctcctttttt aaccctttga ttttgtgtta caagactttc aaagaatttt ccatctaagt
   107941 ttacagtatc tatcccaata tgaattaata tttctaatcc ttcatcagtc actataccat
   108001 atgcatgttt agttggaaat actatagtta tagttccatc tacaggagat ataacagttg
   108061 attttgattc actaggtata actccaaccc ctttacctaa tgctccagag gaaaatgctt
   108121 tgtcattaac ttccccaagt gcaattacct gaccttttgc aacatttcca atactaatag
   108181 ttcttgaatt gttctcgttg attattagtt ctaattcttt ttcattatca cccttaactt
   108241 cttctttttc atctgaaggt attcctagta aataggaagc tgctgttgct gctacaaatg
   108301 atactactac acttattatc ataaatgtaa agttagatac ctctcctcca acaagatacg
   108361 ttggtaatgc agctaatccc caaaccattc catatgtttt aacttgagct aaacctgcta
   108421 ttaaaccacc tatacctcca cctatcatta ctgctacaaa tggtcttcta tatttcataa
   108481 acacaccaaa cactgaaggt tctgtaactc ccataagtgc actaaaactt actgtaccaa
   108541 atagttgttt ttgttcttta ttcctagttt ttaagaaata tccaaacatt gcaccacaaa
   108601 ctgcaatatc agatattgtt gcagcgccca taaaaattgg gtcatacccc aatgttgtca
   108661 cagaatttaa agatataggc atagtaaagt ttgctgcacc aaaaactatt aagagtggtt
   108721 gtaaagctgc atacagtgca acaacactcc aattacccaa tgtatcaatt aaaaacatac
   108781 atgcatagtt taatccatca ccaatccaca taccaatagg tccaaataca gttaatgtaa
   108841 ctgatagagt tattattaaa gataacactg gaactaaaaa atagtgtaat gctttaggta
   108901 atattttttt caaaccttca attataattc ccattgccca tactcctaaa agtattggaa
   108961 taaaactgtt tgcataagtt gtagcctgta aatttatacc aaataaactt aatccttcta
   109021 caccatctat tgatgaagat aataacgtaa ctgctaacac tactgctata tagggattta
   109081 cattaagtct ttttgctgat gctattgcaa ttaatactgg taagaagtga aatgtagcac
   109141 ttcttaatgt atctaatatt tgatatgtag gactgtcact ctgaagtatg ccagacatat
   109201 ttaaaagtgc taatatactt gcaagtatac cagctgcaag tagtacttca attactgaag
   109261 tcatagtctc tgagactacc acaaagatgg aattaaatat acctttgata cctctaattc
   109321 ccttttttct ttcacttttt atacttgatt cacttgaact taaacctatc atagacataa
   109381 actcgttata tacatctaca acatgagtac ctattataat ttgataagca acatcattag
   109441 aaactacatc aattactcct tttaattttt taatttcttc tgtttgagcg atactcctat
   109501 catttaaagt aaatcttaaa cgagtaacac aatgcgctac cgactttata ttttcttttc
   109561 cccctataag ttttattata ttttcattta aatctttata tttcatctga ttttgcctcc
   109621 agtctttgag tcaccctatt tatatgcaac attaaataag tttcttcact tatagatata
   109681 tcgacattaa attgtgattt tatatatttt cttatctttt gaacacttcc aaatgacttt
   109741 ggataaagtt gacttacttg tttatataaa tctatagaat tatcttcatc tattgaagct
   109801 tcatccgaca taattctttg gataaagtac tgtagatgag tagtaaatct catataattt
   109861 gttgaagttt catctagctc tacattaaaa tgatatttta ttatagaaac tatatctgct
   109921 aaagttctca actctacaat tgtactatca tgagactctt tatttacctc catatttaca
   109981 aaatgaagtg ctattgatac tgcctcatca tcaggaagat taattccctt actatcacca
   110041 attaatttta gtgcatgcat tccaatttca aaatgctttg gataaaatct tttaacattc
   110101 cataccaaag gactttttat aaattgattc ttttcacatc ttttaagtaa aaactctaaa
   110161 tggtctagta aggttaagct tagataatca cttgccttta tatttaaagc tttctcacca
   110221 tattcaatta tttcattaat aagaactata ttattggagt ctgagttttg tagtagataa
   110281 ctaaagtgtt ctagcatatc gtatgaatct agtatatatg ttctctctat ttcattttca
   110341 tttattactt gtcccactct ttttttaaag ccaattcctt taacaaatac aataatttca
   110401 tttttacctc tttttactaa ggctacatta ttatttaaaa tctgaattat tttcatatat
   110461 tcctcctttc aataaaaaga actcaagcat atatgtatag agtatccctc tatttttcac
   110521 atatatactt gagttctgcc tgctaatcag taacatactc aaaattatga ctacgtcgcc
   110581 ataattaaaa ttcttttttg tttaatttca tattctatat gttatactat ccatataaat
   110641 ctgtcaacgg tttcctagat atttttttat tctataacta caagctctgt tatgcgatta
   110701 tctgacaagg tttatatact aaattgatac tattttaccg tcactaatat ttataatatt
   110761 gtctgaacca ttatctattt tactattatg aattatcata gtaatgcttt taaatataat
   110821 gaactgtatg ttaacaaata aattcttaag atatattaac atataaagtc ttaagtttag
   110881 ttttatttaa tcactattaa gatatattaa taggtaaagc ttcaaattta gtttcattta
   110941 aatgctagta agatatacta atatataaat acttaaattt agttttattt aaacgctcta
   111001 gtgatatttt taaataaatt ttaataaaaa tcattctatt ttgtcataaa tcatttatct
   111061 ggcttatatt taagatatat tagtggatac tttacacttt ttaattcctc attacttctt
   111121 aaaagtttat tattattttc catatcataa atttgtaaca ttactttatt tacatataga
   111181 gcataataat tatcactata aaaatattga tactcatatc gagggtcatc tttgtaatac
   111241 actttgtata gtattccttc gtaacttgcc ttcttggctt tttcttttgt aatacttttt
   111301 atattatctt ttgaaatatc ttgttttacc atatacttat caatatatgt ttcagcatct
   111361 ttccatggtt taatgcagtt tttatataca acaaaccctg ttacacttat aatacctata
   111421 attaatacta taaaaataac tttatatttt ttcatattaa tcctccttca tttattattt
   111481 aaaactcgat ataatgcact aaaaaaagga tgttatcttt tttattaaaa tataacatcc
   111541 ctcatattta gcatcaatat atatgcccca actggtaggt taaaagaccc cactgtcagt
   111601 actttgatta aattacatgt atgtatatat atttttaaca aatctattat atgtcaactt
   111661 gtctactatt acatataatc tgtaatatta catctgctgc tttttttgag cctcctgcat
   111721 tataaaagct ttttgatagt ttagttgcat tttctttata ttcattgtca tttataactt
   111781 gaaatactgc ttttttaata ctttctgact tatcctcttt taacataatt cctgctccca
   111841 aatgaacaac tctcttagct accattctct gctccgaatg ttgaggaaat aataccatag
   111901 gtactccata atataaactt tcattaacac tattcatccc agaatgagta acaaacacat
   111961 cagttttttg aagtatagca atctgttcaa cactattttt gacctcaaaa ttatttggta
   112021 tatttcctaa acttttaata tcagtacttc taccaacaga cataatcaca tcaacatcag
   112081 aatctttaaa tgcacttata cagtttttat aaaagctaac atttttatta ttaattgtac
   112141 caagcgaaat atatattagc tttctttttc tctctttagg ttcaataatc aactttgata
   112201 ctgatggacc tacaaatgaa tacttactag agaaggtttc taccattggt tgaaattctt
   112261 ttgatgtata tacaatggta tccgtatcat tatcattaga aactatagaa ataaaatttt
   112321 ttacttcata cccttttctt tgtaaaagct ttattttttt attaatgcgt cttacaccaa
   112381 aaatcatacg tataatttct ataaagtttt gtttcatcaa ctttgctgta tgtttattaa
   112441 atgcaaaagt tgttgtagag catacatatg gaatattaag tttttgtgca aataactttc
   112501 cccaaaaact taatgaatct gatacaatgc aatctggatt aaaatcttta agttctctac
   112561 aaactttttc atctaaagaa atagtagtat ctactatcat ctcaattaat ccaggaaaat
   112621 ccttaccaat ttttttctca tctccaggga gtaattctgg aaggtattta tcacaacaaa
   112681 taaattttgc tccagcacct tcaattttat ctttaaattc attaaaagaa taatacaata
   112741 cctcatttcc tctatccact aattcacgaa ctacttcaat agtaggatta gtatgaccat
   112801 atgcaggaat gctaaaaaat actattttac tcatatttca tacctccttt tataattttt
   112861 ttaaatcatt cttctttacg tactactccc acatgaccta taaaatttat caaatcacgt
   112921 tgatgaacaa ttcctaatcc tctttcctca tctccaagta ccaataattt atctcctgca
   112981 tttatattaa atacttctct tgcttctttt ggaatgacaa tctgaccacg ttcaccaact
   113041 acaactgctc caaagaaaaa ctttccttta ggaggtacta taatacctgt tttttcttct
   113101 gaatgattaa ctaagtcatc tagtttgaca ttgtacaatt ttgcaagttt aatgcaacta
   113161 catatatcag gaatagattc tccactttcc cattttgcta ttgattgtct agaaacatta
   113221 agtttttcag ctaattcttc ttgtgtatac tggttgattt ttcttagact ttttaaattc
   113281 tcattaatca tatttttcat acctcctatg tttataatat tggatttatt atttaaattc
   113341 tatcaactaa tcttaacatt catatatata tttatatact tttattatat aaatttatta
   113401 ttcaggtaac atatttcctt aagttatcgt aaacaaaaaa acaagcacaa atctactaac
   113461 tgttgttaaa gtatttaagt taaggtcatt tacattatca aatatttcta cacctttaaa
   113521 ataaccttta tatttataat ctttcataag caaattatta tcccttctca tatacaagtc
   113581 cagttgatgt cttgttgcta aatttaatgg taatcaaatt ctttaccaat aagtataaaa
   113641 attactaatg tgttataatt ttcttaacta ctaaatttta catacatttg aatagaatat
   113701 atcaataaat tactataata aataaaaaat agttagggag atattattat gattttatat
   113761 tttagcggaa ctggtaatag ccgatatgtg gctcgtaaaa ttgcacaaga attaaatgat
   113821 gaactgatat cactaaatca acttataaaa gaggaaaaaa cagatgaatt aatatcagca
   113881 gataaaccat tcatatttgt atgtcctact tatgcatgga gattgccaat agtagttacg
   113941 gattttataa aagaaacaaa atttctagga agcaacagag tttactttgt aatgacttgt
   114001 ggtggagata cagcaaaatc cataaactat attcaaaaac tatgtaaata caaggaatgg
   114061 caattaaaag gtatggcaga aattaaaatg ccagagaatt atatagcttt attttctaca
   114121 acagacaaag aaacatcaaa acaaatgatt aaagaagcag ataaacaaat atataggatt
   114181 atttctgata tacgcaatga aaatgaattt gaaactatta ctcctagtgg cttaggaggt
   114241 acaattaaaa gtggtataat aaatacagtt ttttataaga caattattag tgccaaagga
   114301 tttcattata ctgataaatg cattggctgt ggaaaatgtg tagagctatg tcctttaaat
   114361 aatattaatt taaaaaacaa aaaaccagtt tggaaaaata actgtactca ttgtatggca
   114421 tgtatttgtg gttgccccac tgaagctatt gagtataaaa ataaaactca aaacagagaa
   114481 agatactatt tagaataata tattcttaat atttaaacca ttataaaatg aactataccc
   114541 attgttttat acagttttac atattttaac cgtctcctaa aatgggtata gttcaaaaga
   114601 tagtgtctca aagtgaattt tttagttcct aaatcaatct ttttgtatta aatcaagtat
   114661 aatttaatca ccttttctta tatcattttc actaatatgt tgtattccaa caaatataag
   114721 atttattagc tctgttagtg acttccattg tgattttttt gaatcaagat aataataatt
   114781 tttcgttcct tctctacgca aattaacaat ctctgcttct tttaatatct taagatgatg
   114841 agatacagca ggacgagaaa gatgtgtttt ttttgtgatt tctcccactc gtataccatt
   114901 aaaatcactc tctaaaagag caattaaaat caattgtcta gtctcatcac ccatagcaga
   114961 gatagctttg cgacattctt taaatttaga cgttatttct tgcattcttt gttgacattc
   115021 tttttccata aaatccaccc tctcttataa gtatctaaca gttgctactt ctttctgtgc
   115081 agtacgtcta tatttaacat ctgggtatcc aattactaat gttgtaagaa catgattact
   115141 acgttttaga cctaaaagct tttttaattt tggcgaatta tttgctacat ctgaaaaata
   115201 tccactaaat aaaacaccta gaccataaga ctctgccatt aatgccatat ttgatgctgc
   115261 aagtgaacca ctaatcttat cttttgtaat tatcattatt gcaataggag catgcttaaa
   115321 gaaaaagtta tcgtcaatct caacttcttt tgaatattta atagctatat ttgcaatagg
   115381 ttttattttt ctaaaaaatt tcactgcttc gttttcataa attgactttt tattgtctaa
   115441 aacaatatat gatacatctt gtgaattttt agcactaggg gtatacctac ctgcctcaat
   115501 aatttgcttt ataatttctg aagaaacttc tttatcttta aatttacgta tacttcttct
   115561 agatttaatt gccattagta attcatctga atctagcttt ggcttatttg taagttctat
   115621 aggaggttca tcaaatcctg tcaaggtaat agcttttgtg ggacatattg ctgcacaatg
   115681 tccacacatt agacaatctt gttttttaat tactgacttt ttattttcta taattatatt
   115741 gtttactgga caatcatttt tacaaagccc acatccaatg caaagttctt tgtcaacttc
   115801 tattatatgt tggtatttca tattcattca tctacctttc gtttattagt ttaaactatt
   115861 ataacacgta gacatttttt atattcattg gtaaagaatt tgattaccat taaattttta
   115921 atttctctat taaataaatt tttcatagaa ttttaaaata tcatagttta tacataataa
   115981 aaaagacaag cttaaaacct aagcttgcct ttttcatctt ttattacaaa gtaaattgca
   116041 cttacttccc atacttctta acaattttat tttctaaaat tttatttgtg aatcatacta
   116101 ttgcatcaat acaatgtaaa ataattctat attgattttc tatagttaaa aaagttaaaa
   116161 attatactta ttattaattt ttatactctt cttatttaat acaaagttct tatttaacat
   116221 agatttcttg tctaatatag atttcttatt taacatagat ttcttgtcta atataaatct
   116281 tcaccatttg aagcaattac tttcttatac cattcaaaac ttttcttttt cattcttctt
   116341 agacttccat taccactatt gtctctatct acatatataa atccataacg cttttccatt
   116401 tcacctgtac cagcacttac taaatcaata ggtgcccatg tagtataacc taataaatcg
   116461 accccatcta tagaaactgc atcatgcata gcttttatat gttctcttag ataatcaatt
   116521 ctgtaatcat cttctacata accattttca tcagctatat ctttagcacc taatccattt
   116581 tctacaataa atagtggctt ttcatatcta tcatatacat gatttagtgt aattctcaaa
   116641 cctaatgggt caattggcca tccccattga ctgtatgaaa gatatggatt tttagcagat
   116701 gtaaatatgt ttccttcagc catatcatct gcattttcat cggttctaac acaacggcta
   116761 ttgtaatatg aaaatgaaat aaaatctact gtatatttat tcaataattc aatatcacca
   116821 ggttccattt ctggtgtaat acctttttgc tccatatact ttaacgcata gttaggatat
   116881 ttacctttag attgtacatc cacaaagaag taattttctc tatctaactt tgtagcttcc
   116941 catacatcat taggattaca actataagga tatacacttc cagctgcaaa catacatcca
   117001 attaagttat ttgaatcaac ttcatgtgct attttagttg caattgcact agctacaagc
   117061 tcatgatggg cagcttgata ttttatcttt tcaacatctt caccttcttc aaagcaaata
   117121 ccagcaccca taaatggagc atgtaaaatc atatttattt cattgaaagt tagccagtac
   117181 ttcactaatc ctttatatct tctaaagata acattgcata atttctcata gaaacctatc
   117241 atttttcgat tacgccatgc accatacttt ttaactaaat gcattggaca atcaaagtgt
   117301 gtaatagtga ctaaaggctc tattttatat tttttacact ccttaaatat attttcataa
   117361 aattgaagtc ccttttcatt tggtgtttct tcatcacctt ttggaaatat tcgactccat
   117421 gcaatgctca ttctatatac tttaaatccc atttcagcaa ataactttat atcttcttta
   117481 taattatgat acatatcaat accagtttta gctggataat aatgctcatc atcaaaatca
   117541 aacattttta gtttccctgt aataactgct gttctatctt ttcctgttgg acatacatct
   117601 acatttgcca atcctctttc gtcttcattg tatccacctt cacattgatt ggccgcagta
   117661 gctcctcccc ataaaaaatc attcttaaaa ctcatagtca aagcctccta aaattataac
   117721 caaagcacta gattatgcta atacctcaat ctttactaaa ataagactgt aattagattg
   117781 tcttctaatt caatattttt actttctgtt tctactacat ctaaaaattg tgatgaattt
   117841 gtaataacaa ttggagttac agtactatac cccaactctt ttattgcata aacatcaaag
   117901 tttaatatag tttgaccttt tgtaattcta tcaccttgtt ttatacatgc ctcaaaacct
   117961 cttccctcta attgaatagt atttaatcca atatgaataa gcacttcaac acctgtatca
   118021 gaaagaatcc ctatagcatg tagactagga aataaggttg taactacccc atctactgga
   118081 gcaactactt ttccatcact aggtattatt gccacccctt gacctagtac accagatgca
   118141 aatgctgcat cctctatatc acttaatttt aatacttcac cttttatagg acttgttact
   118201 atttctcttt ctaatttagt agacttgact tcttttattt cttctttacc cttatttctt
   118261 acttttttag actcttctac tttctcatct ttatataaaa tcatagttaa tacaaaagct
   118321 acgaccattg atatagctat accagaaaga gctactatta agttacccat tcccccatct
   118381 ggttcaatca ttgctggaag ttcaaatatt cccattcctc cagttataaa ttttctaaga
   118441 ttaaagaatc catagaaagc gcctccaata ccactagcaa tacaacttat tataaatggt
   118501 ttctttaaag gcaataatat tccataaatt gctggctctg taactccaaa tataccagat
   118561 ataaagtttg gtattgccat ctcttttagt tttttatctt tagtcttgaa aaatattgcc
   118621 aacacaactg ccgaacttgc aaatgtacag gcaaagaatg gcatcataac attatcatat
   118681 cctaaagtca taacattgtt tatatataca ggaataaatc cccagtgcat accaaaaata
   118741 acaagaattt gccaagtaaa tcctactata gcacctgcca atagtggact aaaatctctt
   118801 attgaaatta caatttgtga aataagtgta gaaccaaatg ttgctatagg gcctataact
   118861 ataagtgcaa caggcactgt tattaataat gtaagcattg gcacaaagaa aaacttaact
   118921 aaatttggaa cacacttatt aaataatttt tcacacctag aagcaaaata cactataaat
   118981 ataattggta cgactgttcc agtataatca attgatacaa ttggtagtcc aaagaaatca
   119041 atatatactg gtgatgcaaa cattgttcct ttaaataaag tgtacatcac atctgctcct
   119101 gctgatatac tgctaccttg tatagcagga taacatactg cagctcctag tgctaatccc
   119161 aacattggtt ttaatccaaa cttcttagct gaagtatatc ctaagaacaa aggaaggaat
   119221 gtaaataatg catctcctgc tgcatttatt accatataac caccagatac atcactatat
   119281 aatcccattg ctacaaataa tgcattaaat cctttcaaca taccacaagc tgccattatt
   119341 cctaacactg gctgaaaaat tcctgatatt atatctatac ctttatctag tatattacct
   119401 gattttttag tttctgaact tgtacttgaa tcatctaaac caattatttc aactacatct
   119461 ttatagacag cttcaacatg atttcctata actacttgat attgtcctcc acttttcatt
   119521 acagtaacaa taccttccat gttttttaat acattgtcat ttgctatact ttcatctttt
   119581 aaagtaaaac gtaatcttgt aatacagtgt actaacccac taatattttc ttttccacca
   119641 acattgttta ctatctcttg tgctaattgt ttgtattttc ccatttttct cctccatatt
   119701 tcgtttaaat taattttttg aaatttgaag gtttggccaa ctcaatgtta ccatccaacc
   119761 ataagtataa ttttgttagt atcactcctt tcaatcattc ttaatatact actgaaaaat
   119821 tatttcactt acatatttga gtctttaact actttagcta aatgtattgt taaatatata
   119881 agctcatcat ctgttaatat gtagttatat tgttttttaa tgaaattttt aattttaact
   119941 acacattcaa actctttatt atatttagat ttaataatgc ttagtaattc tttgtctgtc
   120001 tcaccttcat gtgcactatc taaaagcaat ctttgcgcaa aaaactttaa atgcattata
   120061 aatctataat aaaacactga ttcttcatga aattctatcc taaaatgata cctaactatg
   120121 ctaagtactt cttgtattaa cttcgtaata tcagatgcca aagtatgacc ttcacttaac
   120181 tgagcattca ctatatgaaa ggctataaat ccagcctcat cttctggtaa ttttatacct
   120241 gttttgtttt caataatatc tagtgctttc attccaattt caaactcttc tttataaaat
   120301 cttttaattt cccaaaggat tgcatttttt acatttattc cttctttcat cctttgaatt
   120361 gcagaatatg tatggtcact taaggatatg taaatacttt catttagctt cttgtttaac
   120421 tttatttgtg cgtattctat aatttcactt gataatttca tgtgttctat aggaatatta
   120481 ttgagtaatt cttgtagttt gtttgatatt tctttatttg atattctaaa tactttattt
   120541 actttagttt tatctattag attgcctttt gatttttgat aaccaattcc tttacccata
   120601 acgataactt cttcatcatt atcatctaat gtgattacaa cattattgtt taatatctta
   120661 ttaattatca tgattatcct ccttttaagc ataaaaaaag cttatccata ataatgggca
   120721 tactccttcc taggatactg ctattattaa tgaataagct tagcttgtac ttaaacaatt
   120781 actacctaat tccaattaat atgataaaac atcagacata ttttgtcaac tttttatttc
   120841 catatacttc atcaatcttt tatgttgctt tttgtcagtt ctaataatta ttcttattaa
   120901 tattaactta tttctttgca tcaatataaa tacaaaacaa gttatagtca ataataataa
   120961 aaattctatt aatgtaaata gttaatatta tacttcctct actacattat ctgtacttta
   121021 tttaacattt ttttcaactc caaaaacata tgtcataata aatggagcaa ccaatgctat
   121081 aaatataact ataagtccat ttactatatt tgaaatcccc ttttcccctg ataatccaag
   121141 tactgccatt atactatttt gtggaacata taaagtcaca tgagtaattc ctgcaaataa
   121201 ccctgcgatt cctgctccta tagatgctaa catagcaggt tttttaagtt tcaatgtaac
   121261 tccataaaga gctggttcag ttatacccat gatagctgat atccatgcag ctgatgctaa
   121321 ctgtttcatc ttactatctt tgcttcttaa tgctacagct aatgttgcgc caccttgcgc
   121381 taaattagca caaacttgag atattaatat tagcgattct tgtcctgtag ttgcaaatgc
   121441 tgatatgaat ataggagtca atgcatgatg catccctgtc ataacgataa atggcattgc
   121501 agctgctaat aaacaaacca ttataaatcc taaccttcct tgtagtaaat ttattatact
   121561 agctaatccc tctcctaaga aatttcctat tggtccaatt actattaatg ctattggcgc
   121621 acttattaat aatactaaag ttggatttaa aattgttttt aacatgctag gtgttaattt
   121681 atcaatgaat ttttctatat atgacatcat ccatactgtt aaaagtgcag ggataacaga
   121741 tgctgcatat ataacatctt gaattggtac tcctataaat gatatagggt ccccacttga
   121801 tacccatgca gagaaatttg gatgtaaaaa cacacctact actataactg ctattgatgt
   121861 attaacatta aattttttag ctgcatttat tgcaagtatt ataggcataa agtaaaatgc
   121921 tacatctcct attatatcta atactctata tgtacttgaa tttgatgata gccaattaaa
   121981 agtggtgcaa agagataata taacttttat catcccagat gctgtaaggg ctggtatcat
   122041 aggtgtcata caaccagtta ttgtatctat tatattattt aaaatactat tttttttatt
   122101 tttatttttt atatcgcttt taatatctcc tgtggtattt atccccttat ttattattgc
   122161 attaaataca acacctacct cattacctat tatgacctga tattgagttg aagttgagtt
   122221 tactccaact accttatcta tagattttaa tttatctgca ttgactttct tttcatcttt
   122281 taagatgaac cttaatcgtg tcatacaatg agttaagtca attatattat cctttcctcc
   122341 aagtccatca attatcaatt gagctacttc ttcatattta ttcttcatac cttatcctct
   122401 cttttaaaat aatttttaat ataattttaa tccaaattaa tctttttact ccaagcttgt
   122461 aatagtgtat tgtaatttaa taagctagta tcttctacaa tatggtcaca ttttcctagt
   122521 acttcttttt tccccacacc aattgatgtc atattagcag catttatagc tttaatacct
   122581 gaataagaat cttctattcc tacacaatca tattgtttta aatttagaat cttgctccct
   122641 tcaataaaaa tatcaggata tggttttccc ctttttaata aagatggatt tactatacaa
   122701 tcaaattcat cttttattcc caaagattct aatatcctag gtgcattctt actagcagat
   122761 gctattccca atttcacatt atttttttta agttctttta taagctcatt tatccctggt
   122821 aatatatctt tatatgttaa tgagtttatt aactttatat aaatgttatt tttttcttca
   122881 cacaccttta acttttcctc tttagtataa ctatctactt tattagcaga taacaatatt
   122941 ttatctaatg attcttccct gcttatgcct tttaattttt cattaaaagt ttcatctatt
   123001 tctatatcta gcgattttgc tatctcatgc caagcttcaa aatgatactt agcagtatct
   123061 gttataactc catcaagatc aaataagact gctttcatat taattactcc ctttaaaaca
   123121 attattaact tctattatgt tctttgataa ttttatttct ttattatcat gtaatatggt
   123181 tatatttcta cctttgatta acttataata agatttatat tcttctatat ttacagaaac
   123241 taaattatct ctaaataata tattaaattt aatatttttc cagtgtttag gtatccttgg
   123301 ttcaaaactt aatttatcat tttttatttt cattcctgca aatccatata ctaaactcat
   123361 ccatgttcca cccatattag cagcatgtat tccatcactt gtattttttt gtaggttatt
   123421 aatatccatt aatgctgtgt ccataaaata gttataagct ttttcatgat ttccaatttc
   123481 acttgccatc attccaaata ttgaccttga caaagatgaa tcatgagtag ttattttttc
   123541 atagtattca aaatctctct tcttttgttc taagctaaac tcatcaggga aaagcaacgc
   123601 tgaaagtaca gtatctgctt gtttatttac ttgatatcta tatattgtta atggatgata
   123661 atgaagaagt aatggatatt tttcttttgg tgtattttta aaatcccata ctgatttttc
   123721 taaaaatgaa tcgtcttgtt tgcttatttt cttctcttca tcatatggta aatacatatt
   123781 ttgagcggca tttatccagt ttttcacttc atattcttgt aatttaactt ttctaaatac
   123841 ttcatttgaa aataaattat gcttaaattt atctaccatc tgtactgcat atattaaatt
   123901 atacttagcc attaaattag tgtagtaatt attgtttact attgctgtat attcatctgg
   123961 tcctgttact ccatttatac agaatttatt attttttgat tctatataat ctccaaattc
   124021 aacccaaaat cttgctgttt ctataagaat ttctaagcca ttttgcatca taaattcttc
   124081 atcattagtt gcttcaaaat acgttctaat accataagct atatctgcat ttatatgaaa
   124141 ctgtgctgtt cctgctggat aatatgcact gcattcatta ccatttatag ttctccatga
   124201 atataagcaa cctttattta ttccaagttc tctagctctt tctcttgatg catctaagat
   124261 tgagtatcta taatctaata aagctctggc aatggatggt tgagtatata taaaaaatgg
   124321 taatatatac atttctgtat cccagaaata atgcccctca tagccatcac ctgtaagacc
   124381 ttttgctgat atattagtct ttccatctct tcctactgat tgataaatat gaaataaatt
   124441 ggtttttatc cccagttgaa gagttttatc gccttcaatc tctacatcag aggttgtcca
   124501 aaactcattc attttctttc tctgaatatt tttgacttta tcaaatccaa tattacatgc
   124561 ttctcttaaa accaattcta atgattttaa atgtgcttct gattctaaac attttaacga
   124621 ctccagacat tctaatgatt ttaaacgttc ttcatttaga tttaaatcag aatatactct
   124681 actatagcct acaacaattt ctaaatttaa cctttcatct tttttggcat agacttctat
   124741 ttctcgctca atacattcat ctgaaacatt ataggtctca tttgtaatat ttccacaagt
   124801 aatatatgaa tcaaacccac accataaccc taatctactt ctttctgtaa ctaaattcat
   124861 aaaatactta ttgttatatt tttttacatt aattttaaat tgcttcttat ttatagatgc
   124921 cactcttggg tcctctactt tttcacaatt aaacttctcc atactatttt ccattttatt
   124981 tttaaatttt atttttccat taaaattcaa tggtgttatt gtatatgaaa tcatcattaa
   125041 ttcttctata tcttgtgata ctaatctttc tatagatact ttaacttttc taccacgaga
   125101 gttttcccat ataaatgaac gtttaagaat cccttcattt aaatctagta tcctagaatg
   125161 ttcacttgta ataccttttg ataaatcaaa gcaatctcct tcaatagaga aatttactat
   125221 atgagcatta ggtaacttac agatagtctg attttgtttt gcatagccat aagcgctttc
   125281 tccatatgta attggagatt cttcataaaa tccatttaca tatgtaccca tattatctac
   125341 atactcatta ttaaaatcag actcttcaat attccctcta actcctaaat gtccattagc
   125401 tagtgtaaac agcgtttcat tggattttat tgtatcctta ctaaaccctt gctctataag
   125461 ttggtcctta gtagtaaata ttaaactcat aataacactc ctttcttaaa atgaattatg
   125521 catgagtttc catgtcatta aataaaattt ctccatctgt ttctattact tttttatacc
   125581 aaaaagcact ttgtttttta gttcttttaa atgagcctct tccatagtca tcagaatcaa
   125641 catgaattat gccatatcgt tttgtcatct ttccttcact tacactggtt acatctatac
   125701 atccccacat aagatatcct ataagctcta ttccatcttc atttactcca ataatcatat
   125761 ttttaatatg ttctttcatg taatctatac gataatcatc aattattttt ccatcttcat
   125821 ttacaatatc ttgagtaccc agtccatttt ctactataaa taaaggtaac tgataccttt
   125881 catataactg attcatagtt attcttaacc ctacagggtc gttttgccat acactatttg
   125941 attttggaag atatggattt tttatggttt ctggtccgtt tgcctttgat ttttctaaac
   126001 cttcagctct agctgaacta caagcactag aataatagct aaaagataca aaatctacag
   126061 tattatcctt aagtgttgat aaatcttcct cttttattat taattttatt tgatttgatt
   126121 ttaaataata attcataaag cttggataca cacccctaac ttgaacatca gaaaaaaaca
   126181 aattatgcct ttcatgctct aaagttgcaa atacatcttc tggtttacat gtgtatggat
   126241 atgtttttcc agcagctaac atacacccaa acttaaattc tttatttata taccttccta
   126301 tttctatagt ttttgcatta gctaataatt gatggtgtgc tgcttgatat tttttctgaa
   126361 gttgattttc atcagctata aaagttaacc cagcacctat atatggaata tgcattatca
   126421 tattcatttc gttaaaagga atccagtatt tgacatcatc tttatatctt ttcatcaaaa
   126481 tttcagcata ttttacatat aaattaacca ctcttctatc agcccaccca gaatattttt
   126541 caactagaga ataaggtata tcaaagtggc ttatcgttat taatggctct atattatatt
   126601 tttttaattc ttttattata ttatcataaa gttgtaatcc cttttcattt ggttcacttt
   126661 cttctcctgt tgggaatatt cttgaccaag atatagaaaa tctaaaacat ttaaaaccta
   126721 tttctgctaa cattgctata tcttctttgt aattttcaca aaatttaatc cctatatgac
   126781 ttggatagta ttgattattt tcatttaaaa tattatcctc atctaaacat acatctttta
   126841 atctcttatc atttaatggg agtttatcaa actgagataa accttttcct ccatctaaat
   126901 aaaatccttc actttgattg gctgaaattg cccctcccca taaaaaatct ttccctaatt
   126961 tcatgcatct acctctttca caatataaag ttttataatt tctatctcat atttagttat
   127021 aagatttttt tcatactata ccttttaaat aattcaattt aaaaatccaa ttatattttt
   127081 tactcactat aattaatatt gtctattttc aatactttca tattaaatat caatattaga
   127141 tatctatgta tataattata tatatagcaa atacattttt atatcctcat tattgttatc
   127201 ttattctcat atattgctgt atatttatat ataaaaggat ttatttcgaa atagaggttg
   127261 tattgagaac taaaatttag actttataaa tgaatggaat tttcattgtt gaaatactga
   127321 aaatatattt gatttcatat aaaagaaagt gcctcatgaa ctaagttcat tttgagacac
   127381 tccctttttt ttccaagcta ttatatcttt ttttaaattc taatggagag actccaattt
   127441 tttctttaaa tactctgtaa aaagtttttg tacttccaaa accaacttct tcaaccaaat
   127501 ctacaatgga tatattgtta ttaagtaata tccactttgc tttttctaat ctatattcat
   127561 ttaaaaaagt tataaatgtt tttcctgtat tttttttgaa aaatttagta aaataatata
   127621 cactaaatcc tacttcataa gacacatctt ctaattttat aggttgttta taattttttt
   127681 ctacatattt gaatatattg ttcaatctct ctaatatctc tttagagttt atttcatatt
   127741 ctactgtatt ttcttcaatt tctctaggaa tctgtctata taatatagga attaaattat
   127801 acattttacc ttttattgca taactatatc cttctatttt gtataggtct tcattgtaga
   127861 tatctatcaa tatatcgaaa actgactttc ttatttttgc tgaccaatct ctactacatt
   127921 tttttatact actgaataaa ttaattaagc tttctttttc ctcatttaat aaaattaaat
   127981 cattaaaaaa agatatatca aattgaaata ctaatctttc acttcccggt gatggcagta
   128041 tataatgaat atctcctcct tttattatca ctatctcgcc tttttttaat tgcataggct
   128101 tgtcatttat aaatagattt actacacctt ctgttataag taatatttct atttccttat
   128161 gccagtgtgg tgttgttaat acttctcctt catttgatag actacgaaaa ggaaattcat
   128221 gtattaattt aggcatttct aaatatattg ccatatatat cctctcctct ctagtgtcta
   128281 aaaattaatt tatactattt aaaagaatac acattttatt ttaaaaacct caaatttata
   128341 atttatacaa tacctacact tcataaaata tatctaatat ttcttttttt gtatttgcat
   128401 ttaaaagtct ttcacctata tttttaccac ctaaaatgta agctatttta gatattaata
   128461 tcatatcttc attgcctcca gattgtggca tagcaaacat aattattacc tttacaggta
   128521 agccatctat acttggccaa tctacacctt ttttagattt acctattact atggatgcct
   128581 tctttacata ttttgttttt ccatgtggca tggccatttg ataacctatg ctagtagagc
   128641 ctattttttc tctatgtttt aatgccttta taaacttact ttttaaagtt atacttcctg
   128701 tatcgtataa tttatctact aattcctgta ttatgccttc ttttgtagtt gatttcaaat
   128761 ctaaaataat gctttcttct gttaatattt ctctaatttc catttgtttt ctccaataga
   128821 ttattcttaa ataatatttt aatatcctct ttgctcttgc aatctattat atagtttata
   128881 tagttctttt gcctatataa atttgatatt tccattaaaa tattctttaa aaaactttga
   128941 ttactaattt ctaaataaat atcaatcgtt ctagtaacat tattgatatt cattatccct
   129001 ttatttaatt tactttgctt atgtatatat atatttaaat cttttcctaa tgaaatagat
   129061 tcatatttac tccctaaatt cacacctatt aaactttcca tctgagctac atcaaaatta
   129121 aaatatatat tatcttctaa atacattcct aataagtttg tatctaagtt catacttttt
   129181 ttaggtttag aaagtaaagc attttttata ttattaatat cattttccat taatagtact
   129241 gatacaacta tatgtaattt atctttcaaa tctaagttta cagtagatat tacaaagtct
   129301 acatcatcta aatttcgctt actcaactga tgcataggaa ctatatcaac tatagtccat
   129361 tcaggaaatt ctctttttaa tctggctgca aggagctgag atgttccata tcctgtatga
   129421 caaactacta ttactctttt actactcata tttctttcta ttgccgcttg aaaatatgtg
   129481 gcaagatacg aaacttcatc tatactaata ttcttcagat tatcatactc tgtcataata
   129541 tttatagtca tcatacataa tcccagcaca tctgaaaatc gctcttgtat ctctcctaac
   129601 aacgggtttt ttatttggat atcatatctt aatctgttta acataggcct tacatgagat
   129661 gctagacttt tctgtagtaa gtcatcattt ttgagattta tatttaaaaa tcgtgacata
   129721 ttctctatca ttatagatga aactttttca ctttcactta atctatctga ttcatcattg
   129781 taactactat tattttcgct actaaatcca gatgatacca aaaaaatata tatgtacata
   129841 atttcatctt tcgttatttt tactctgtat ctattttcta ttttatctat aagtgtactg
   129901 acatttttat atactaactc gtccaaatta tctacagaaa tttctctttc ttcctgagat
   129961 tctatttgat taccttcttc gattctcttt aaacatataa gtatatgggt aattaaattt
   130021 atatagtagg gctgatttat agtatatcct aaattgtatt ctaattctgt aattatagtt
   130081 tctacaaata taatagaatc aatatcaaat aaatttatca atgcactaaa tgtagcagaa
   130141 tctaatcttg ttaactcaga tatatcatgt tgttcataat tttcatctaa taaatcatcc
   130201 atcattttgg caatcgattt ccttatatcc acttctttac ctataatctt agttccttct
   130261 aaagttttat taagttttaa attgtattct tttatccatg cctctatata tttaaaatca
   130321 tttactatag aagttttact tacataatat ttatctgcta actgatttat tgaagttata
   130381 ttatttgaat ttagtaataa atactttaaa atctccattc ttctctgctc tactgataaa
   130441 gatttatttt ccttcgattg tatatttaaa ctattaatta agtctatatt cttctttgct
   130501 aaatcttcta ttaatattcc atagcctctt tttatatcaa tttttatatt aaaagtttct
   130561 aaatatttct caatcatctt caaatctttt tgtaaagttt tatcagatac atttaattta
   130621 tctttaaagt attttatagg tctatattta ttttcgttta gtatcaattt aagaaattca
   130681 atttgcctcg acacaagcat acttagccct ccctacctat gtttatatat ttctatgttt
   130741 atattaagta ctattttaac atatttacta actatgtaaa attactaaga tacttctgta
   130801 tcataaaaga atttatgata caccttacta aattgatata atagataata taatttcaaa
   130861 ataaaaatta aggatattta gaagtagtta actgattcta aatatcctta ttattattct
   130921 atatacttgt tgttattcca ttaattattt ttactattat ttataattta agcaccaagt
   130981 aattcaactt cactattttc actccttata gtaggctttg ttacccctat aatcaatgct
   131041 gttattactg taccaatcaa tatacaagct atatatgcaa ctggtgcact acatgcaaat
   131101 ggtattacaa atattccccc atgtggtgcc attaaagtta tatctaaact catacatatt
   131161 gccccaccta tacaagaacc aattatattt gctggaatca ttcttaatgg gtctgcagct
   131221 gcaaacggta ttgcgccttc tgttatgaaa gatgccccca ttatagcagt tggtagagat
   131281 gactttcttt cttctttagt aaacttattt ttgaatatta acatagctaa tgatattgct
   131341 aatggaggta ccattcctcc taccattaat gctgctgatg gttcaaatat attacttgaa
   131401 aacatagcca atccaaaagc tgaagctgtt ttattaatag gtcctcccat atctgatgcc
   131461 atcatccctg ctaatattgc acctaataat actttattag ttccagacat actttgtaac
   131521 caagattgta aagcttcatt taaccatgat gctggcgtat taactacaaa gaacattata
   131581 gctccactcg taaatgcaat caaaagtgga catatcaata cagcatttac tccttttaat
   131641 acatctggta aattttctgt tatcttcatt actcctaaag ctatatatcc tgttgcaaaa
   131701 ccagctacca taccacctat aaaacctgaa cctccaataa ctgccataaa accagctatc
   131761 atagcaggag caaatccttg tcttcctgct attgatttag ctataaaacc agcaagtatc
   131821 ggtataaata acttaaatgc tgaatcgcct ccaagttgtc caaagaatcc agctataggg
   131881 ttgtatgttt catgagttgg gtctgatgca aatataccaa acataaaagc tatggctata
   131941 ccaattccac ctatcactac aaatggaatc atttcagata caccactcat taagtgctta
   132001 taaacaccct ttttctctga cccttgaagc atttcttttg aatcttctaa agtctttcca
   132061 cctggtttaa tttctttagc cattccattt tcagcgttta taattaaatt tttagctaat
   132121 gaaacattct tagcatctac tactttcttt tgtagcactt tctttcctat aaatctactc
   132181 agttgaactt ctttatcaca agctattatt acgtattcag ctctttttat atcttcttta
   132241 gtcaacttat tttctacacc tatagctcca tgagtttcta ttttaacttc atatccaagt
   132301 tcttgacctg cttcctctaa tgcttttttt gccataaaag tatgagctat acctgttgga
   132361 catcctgtta tcccaactat aagaccttta ttttgattat tactaaaatc tcctgattct
   132421 gcagctttat tattaaatat acttaatatt tcgtatggag tttctgcact tagtagatgc
   132481 ttaagaaaat tttcttccat tagatatgta gctagtttag atataatctt aagatgttca
   132541 tctgaacctg attctggaac tgctatcata aaaaatgacc taagtttttg accatcatta
   132601 tctgtaatct ctctatctgt tcttaaatac atcattacag gttgatttac tacatttgac
   132661 ttagcatgag gtatagctat taaattaccc atattagttg atgattcttt ttcgcgttta
   132721 ttaatatcag ctataaactt ttctttatta tttatataat ttccatggta caaatgattt
   132781 gccatataat taattacatc tttttttgtt tgtaattgtt tatctagtac aatacaatcg
   132841 cttcgtagta ttttacctat cataaattct cccctccttt taatttgtta cctcataatt
   132901 aatttttatt tttataaaag cactttaact tatctatagc attgaataga ttcattttaa
   132961 actctataac ttctcttgca gattttatac atggtggata tatagtatct ggctcatata
   133021 agcttgggtt ttgttctaat agttctctgc acttcttata atatgccgac ttaacatcac
   133081 tcgaaatatt tattttacaa actcctagtt taactgcttg tgctatttct tcatctggat
   133141 ttgatgaacc accatgaaga actaaaggta tatctactac ttctcttatt tcttttaata
   133201 aatctagttt aagctctggt ttaaaacctt ttggatatat accatgtgct gtaccaattg
   133261 caatagctag tgtttctact cctgtttttt caacaaaatc cttagctaat tttgaatctg
   133321 tatatattat ttcatcagta ccaccttcac ttgatgttcc tgttgttcct attgttccaa
   133381 gttctccttc tacggacaca tttaatcctt ttgctatttc tactatttcc ctagatatag
   133441 ctacattctc ttcatatggt aaatgtgagc catcaatcat tactgatgta aatccacatc
   133501 ttattgccct taatatttgt tctttatttt ccccatgatc taagtgtatt accataggta
   133561 ctttactctt atttgcttct tctatgcaac tagctacaaa gctatctcct agaaaactta
   133621 gctcatttgg gtgtattgca agtattactg gtgcattttt ttcgttagca ctctgtacta
   133681 ctgctttaag tatttgccca cttcctatat taaatgctgg tacagcaaat tgattttctt
   133741 gtgccacttt tagcatttcc ttcatattta ttaacatatt gtacctcccg atattttaaa
   133801 ttttaggttg aatttgcttc ttgttaattt tgttgttgtt tatatttaaa taatatcagg
   133861 gtaatgaaga tcagaaaata acaatcgttt tccttagtga aaaggaactt ttattataca
   133921 ctctaagttc cttttcacta aataattttt agaagtctgc atcatagtta attatatttc
   133981 tcatatacat cttctctttt taagtgtttt atgctttagc cttcttaact ctttccaata
   134041 aacaattaga tagtttagct ataaatttaa agatattcgt atcgtagtta gcactatatt
   134101 ggaatcaaaa aaattaaaaa gatactagat agcagatacc aattattaat acaccataag
   134161 tcatagatac ttctatctta aattcttgca tattagttac aatagaaatt aaataggact
   134221 attataaaaa tcgtaaggta agactgacat ttattctcca actatacaaa tgaatactaa
   134281 tatagctcct tctaattaaa tcatactata taaatcatat atatcccaat ttttaacctt
   134341 acctctttat atttactttt taataaaaaa agatattgac taaatagtca atatctttta
   134401 gtggatttaa atacccacga ttttaatcta atttttaatg gtttccttaa cataatctta
   134461 taaatattaa aatttatcta cctataaaat ttctccatta aattttatta catttttata
   134521 ccaatcaaaa gatttctttt tatatctttt aagagtccca cttccatcat tatttttatc
   134581 tacatatata aatccatatc tttttttcat ctctcctgtt gtaaatgata ctggatctat
   134641 acatccccaa acagtatacc ctataacatc aacaccatca atttcaatgg catttttcat
   134701 ttcccttata tgactagcca aatactctat tctataatca tcatctacag tattatctga
   134761 ttttaataca tcttctgctc caaatccatt ttcaacaatg aatataggaa gttcatactt
   134821 atcatatagt tgattaaggt aaaaccttag acctacaggg tctatattcc atccccaatc
   134881 agaaattttt aagtatggat tttcaactac atcagccttt cctgccgagt cattacctgt
   134941 tgttttcata gttttatcag ctgttactac agtagttaaa taataactac atccaataaa
   135001 atcaactatt ccttctttta ggattttttt atcttcctct gtaatatcta aattataatt
   135061 ttttctttcc cataatttta gtgtatgtct tggataatga cctcttacat gtacatcagt
   135121 aaacataagt tgttctctat tagattctac aaatttaatc atatcctctg gattgcaaga
   135181 ataaggataa cttggcatag ctgccaccat acatccaatt ttaaactcag ggtttatttc
   135241 atgacctatc ttcacaacct ttgcacttgc tacaaattca taatgaactg cttgatacat
   135301 aacttcttct ttgtcttcct gctcttcata gattattccc gagttagtaa atgcataaat
   135361 aggatttgta gttatacttt gattatttat ttcattgaaa gtcatccagt acttaacttt
   135421 atccttgtat ctattcatta ccacttcaca gaagtttaca aagaaatcta ttaattttct
   135481 atttctccat ccaccatagt tttttactaa atgataaggc atttcaaaat gacttaaagt
   135541 tattactggc tctatattgt attttaataa ttcatcaaat aaatcatcat aaaatttcaa
   135601 tccatcttca tttggagttg attcatcgcc ctttggaaaa attctagtcc aagcaatact
   135661 tgtcctaaaa cacttatatc ccatttcagc aaataatcta atatcttctt tataattttc
   135721 ataaaaatta atggcttcat gattaggata atttaaaccc tcaattactc catctgttat
   135781 ctttcttgaa atagtatgag tacctgcagt catcacatct gcaatactta ttccttttcc
   135841 acctttatta tatccacctt caacttgatg ggcagcaact gcccctcccc ataaaaattc
   135901 ttgattagat ttcatagaaa tatctcctcc agtataatta ataaacaact tttattacag
   135961 gcgttccaat tttaatatgt ttattttctt taactattac atctgctaaa tcatttgtgt
   136021 tagttagtat tattggtgta acaatactat acccttcacc ttctatttta tccttatcga
   136081 aagaaacaat cctatctcct tttttaactt tatctcctgc attcatatga gtagtataat
   136141 actttccatc taacatagta gtgttaagac ctacgtgtat taatatttct acacctttat
   136201 cagaggtaat tcctattgca tgctttgtag gaaaaatagt tgttattgtt ccatttacag
   136261 gagacacaac ttctcccttt gaaggtatta ttgccatgcc attacctata gttcctgatg
   136321 caaacacttc atcttctact tcagataact ctatttgtaa gccttctatt ggagataata
   136381 tctcctctat accatcatct tcatcaaaag caacttcttc attaccttct tcacattctt
   136441 ctttactcaa tccaaagaaa tatgacaata ctgctcctag cactaatgct gttcctattc
   136501 ctaatatata tcctaggaat ggtgtataaa ctggcattga gaatatatta tgaaatacat
   136561 atgcattaca agtaacacca aatacaccac atataccacc accaatacct cctgcaatag
   136621 ccaatactgg aatcattctt ctgtatctta atataattcc ataaattata ggttctgtta
   136681 ctcctgctaa aattcctgta actgctgttg aacctgctaa tacctttaat tctgaatttt
   136741 ttttagattt tctaaccttc ataaataaac ctattgcaat acctatttga gcaaatactg
   136801 ctgcaacagc tgcaccttct attgggtctc cacccattgt agccaagttt tgcaaagtaa
   136861 ttggtgtgaa gccccagtgt agtcctaata ctgctaagaa tggcatagct atacctattg
   136921 cagctcctgc aattactctg ttaaattcaa ataatcccat aaaaatatta gatattatat
   136981 caccaacatt agttgctatt ggtccaaata aaatcacagt taaaggaacc atgactaata
   137041 atgatatcat cggtactaag aataattgta aatcctttaa tataatcttc tttaaaagct
   137101 tatcaacata gtaataaata aatatagcaa caaatatagg gaatacacca aaagaataat
   137161 caattgctgt tactggcatc ccaaaaaagt tagcaccacc ttttgctcca attaaagctg
   137221 taaaattagg atctaacaaa gctgctccta tcactccacc aacatatgga tttgcactaa
   137281 gttttatagc taaggtaata cctaagaata ttggtaaaaa gtaaaataca gcatttccag
   137341 ctgctgataa tatagcataa gtgctacttg tatcagacat ccatttcata gtagttagta
   137401 ttgtaagcag tgccttaatc attcctgctc cagctagtgc aggtattaat ggagaaaatg
   137461 cacctgaaat tgattcaaat attttagaaa ctattgaaac tttttctccc tgttcttcag
   137521 tattttgtcc ttgtaaaact gcttttttta taatagtttc aaagtaatca gttactttat
   137581 ttccaattac cacttggtat tgtcctccac tatttactac agatagtaca tccttcattt
   137641 ttaataactc ttctttgtta gtttttgaag tatctttcag cttaaaacgt agcctagttg
   137701 cacaatgaac taaactaata atattttctt ttcctccgac atattttata atttcttcag
   137761 actctctttc aaatttcata caatctatcc tccattttca taattttatg tataaaaata
   137821 gacctaaatc aatacaatta gatattaaaa actaattata ccaacttagg tcatgcctgc
   137881 caaacagtaa caatcctaca ctatttactt tatatttgat aatctattta aatgcaatgt
   137941 taagtacaac aattcatttt cgctaatttt ccatttatac gtcttttcaa gaaagttttt
   138001 tacttttttt gcacaattgt aatcactttt atatttcatt tttattatat ctaacaatga
   138061 tgtgtctaca tttgtattct caccacttaa ttgccttttt ataaaacatc ttaaatgact
   138121 tataaacctt gtgtagtcaa aggaatattc atttatttcg attccatagt gatactttat
   138181 gatatctaat acattatcaa ttatcttagt taacaataaa gtttcttgca tatcttttgt
   138241 ttctaactga gcatttgcaa agtgtatcgt aataaatgct atttcagaat caggaatatt
   138301 tttatctaat ttttttctta aatattctac agctttctta ctaaaattat attcattagg
   138361 atatatttgt cttatttccc attgaagcgg gctttgaaat actaatcctt catccattct
   138421 ttttaacata taacttatat gttctgataa tgtgattaat atactatcat taagattatc
   138481 acccaattct tctcttccca tttgaataat ttcacttaca agctcaacat cttttaaagg
   138541 tatcctatct agtagtgaat gtaattcact taatgattcc tcattattga atataaattt
   138601 cttttcaatt ttatctattt caactaaatc tttagtaaat ttattaaatc caattccttt
   138661 tcccattaat attacttctt gtcctgaatt atctctggca aatacaacat tattattaaa
   138721 tactttcttt ataaccataa actacctcca ctgtaaacaa aaagagacct aaaaaagact
   138781 aataatactt taagcattta ctagaatttt taggtcacgc ctgattacat ctatctcagt
   138841 aacgatccgt ttatttatct gtatgataac tttattttca ttataaaata ataactgtac
   138901 ttagtcaata tataatttca tgtatatctc tactagatta ttgtcataag tattcactca
   138961 ttctactttt tttatatgat ttatataatc taaacatgct aaaataagtt ttgaagttat
   139021 ttcataacaa aattaaggtc atatattttt tattatatat actcgattat ttatccatat
   139081 ataaggaaat cctttatttt gtaactacaa taactgccta agttttgatt ttcttttatc
   139141 ttttacttct ccttattaaa aaataattct tttactgttg tatctaaggc tctaaacagt
   139201 ttaatcatca aatcattgtt tggattatta accttaccta acttactata tagcttttta
   139261 actgtatatc tttaatagac cttttggtat gctaatccat tttataacac taattcttga
   139321 atcaagcata tattaccctt acaaagcata tcttataccc tctgtagagt tatgatagtt
   139381 gatgaacctg aagaaattac ccttacaaga catatctgat acaacctcac ttattagttt
   139441 atatgtttca gaagtatata aataacacat acttacttcc aaattcttaa ctgttttctt
   139501 agaaaattct aatatgattt ccttacatag cgattataac agtgtcttat tagatttcat
   139561 aaaattatac atcctcttta catttaaact ttaaattttt atctattgtt tattaattaa
   139621 ctgtatattc actatttttt atcatttata atactcttct tattatctta aaacaattat
   139681 aatctaacct taatcacata ttatataaat atttttaata aacttaataa ttaaataact
   139741 atatttcatt atccatctta taaatgttta taaactttcc actattcttc ttcaataaat
   139801 tcaaacaaat tttctactgt agtatcgaat actttagcga tatccatagc taacttcaaa
   139861 gaaggattat atttcccatt ttctaagtgt acaatagtct cacgtcttac cccaaccaat
   139921 ttagcaagtt cattttgcgt cataaggagt ttttctctgt attctttaat ttttgttttc
   139981 atcaatattg ccacttagtt acatatcccc cttttatcaa agtatatata caacactaat
   140041 ttagataaag actgtataaa taaaactgca atgaggatta tacctgtatc taggtttgat
   140101 atatttatat tacttgtata aggtgttgca aagaaaatag atattattac tatagaatat
   140161 atcattattt ttattgcaat cttattaacc tttgatagta taaattcaga tgattcatca
   140221 actaaatcat tatatttttt attataaatt gaataacatc caaaaactat taaccaactc
   140281 aaccctggaa gagaagcagt gcttggacct ttaaaaatat atttagtagc taatacagct
   140341 ataaaaaaat aagttattac aagtaattcc aagatgagct ttgatcttac accagtttct
   140401 gatttagaca tataaaatca ctccttaaaa gttatatatt ctctatattt tttaattttt
   140461 gttttcataa atattattac ttagttacat attccctttt tatcaaaata tatatacaaa
   140521 attaattttg acaaagattg tatgaataaa actgctaaga gcattatacc tatatccaaa
   140581 tttgatatgt ccaaagctct tgtatgatta actgaaaaga gaacagctat tacaaacata
   140641 gagtataaca ttatttttga tgcaatctga tcaaccttta acaacataag ttttgatgcc
   140701 tcatcaacta aatcattata ttttttatta tacatgagat tgcacaccca aaaagctaat
   140761 aaaattataa aagtaaacca aataccttct cctttaaaaa taaacttttg taataacaag
   140821 tctataaaaa aataggatag catcattaac tctgaaacaa gatttgcctt aacaccaagc
   140881 tctgatttag acatataaaa tcacccctta aaagttatat atttataaca ccttatgtta
   140941 taaatatata acttttccaa ataaattgca atagaatatt gaaaatattc aataatatct
   141001 tcataaatat attttataaa agattttata ataaacatta caatacagta ctaaattagt
   141061 ttatttcatc tatcatctca atcatatcat ttactaacta ttattctaat gagcatataa
   141121 cattgaaatt tataattata caacctaaat gcctcccaat gtaccctcaa aaacatcatt
   141181 tcctgtaact gaagtataat tggtatattt tatcactgaa ataaaaaaac actagtcaat
   141241 aaagactagt attttcaagt attttatcat tcttttttta attattggag acgaatcatc
   141301 agaataatgc ccataattta tctatatgca aattctaaat ttaaaataaa gtttcagatg
   141361 aatttactat tctatgctat gatacaacag aaaaaactga aatctatgag tggtagcttc
   141421 atataatcct agttcctaca gtaatcaaaa ctgaggttta ttctatatgt aactaatgat
   141481 aaattttatt ataggagtta ttagctctat ttgtgttact ataagtagta attatatata
   141541 tgataatttt ttaacacttt tctgttggct atatagaaag tggtttagaa tttaaattta
   141601 aataaaattt aaattcacca aaaagaaatc tatattagaa accactactc tattccacca
   141661 gattgtaatt ttttgttctt tatatcacaa tgctatacca tattaaattg aaaaataaat
   141721 tttaaattta tttgttacaa ttcattaatt caaaaaaata ttttgagtca tgagttttgt
   141781 aaagatttag ccatgaattt aatgcttctg attcaaaaac atctaaaagt ttcaatactt
   141841 gaaacgtaaa ttccaacgga gcgattcctg aagccgtaac taaattttca tcactaacta
   141901 cagattccgt tttataatat ttttctccag agtaatttgg tattaccatt ttaagaaact
   141961 ctaagtcatt acttgtatga tttctaacat ctaatagccc ttttttagct aggcctaatg
   142021 tagctccaca tatggctgca actatagaat cttgtattaa agcttcttta actttttcta
   142081 atattatctc atgattcatg gataaccaag tatttccccc tggcaaaatt aataaatcct
   142141 tactttccat attaaactca tcaactgtaa tttcaggtaa aacttttaac ccacccattg
   142201 ttataatagg atttttatca attcctacag taattacttt tagtggcttt aatccttttt
   142261 taaaatatct tcctgtattt aattcagcag ttaaatatgc cacttcccaa tctgacatag
   142321 tatcaaatac atacatatat acttttctat ttttcatctt ttttactcct tatgtaatta
   142381 atttatatat ctaattataa aataacttaa ctgacatatt cagtcagtta agctattaaa
   142441 tttgataata atcaattaat tctagtaaat cttcaattat tttcttccta aggcttagtg
   142501 gttcaattac ttgtattgtc tttctataag gtaaaagtat atttggtatg tacttgcata
   142561 ttacttcctc ttcgagcata aatattattt tattaggtgt tctttctttc aagtagtgcc
   142621 ctaaaaacca atgttggctt aaactatcta aagaatctat actcccttct acaactaatt
   142681 gaattatatt ttgttcatct tttatttcag gtataattcc ttttataaaa aattcacttg
   142741 ctgaaaattc tgtcggtctg ttaaatgtat ttgatgtttc tatgatttca ctaatcctat
   142801 ccactctgaa actacgtacc tcatttctca tattacaaaa tgcaactacg taccaattat
   142861 tattccagtg aacaattcca tatggattta taattctata ttttgatttt gctttacgac
   142921 ctgcatgata gttgatttct acagatagct cctttaatat actattctcc aattttttaa
   142981 gtactgattc tatagacact ttcccaattg gttttattac ttcaagggct cttaagtggt
   143041 ttgttaaaat atcttcttgg ttttgatttg aatacatttt taactttaat atcgatttat
   143101 ctaatgcttc gtctaagaag tatccttctt ttttagcaaa tagagctgca tgaagtagag
   143161 atttttgctc ttctatatca aaaaaaagag gtgctttagt aaaattattt aaaaggctat
   143221 acccaccatt atgacctgta tctgaaatta taggtactcc actcgcagaa agtacatcaa
   143281 tatatcggta cactgttctt atatttattt ctaatttttc agctatctgt tttgctgtaa
   143341 tttttttgtc agagtttaac atccacagta tagacatcat attgtcaatt ttagccatgt
   143401 agtttcacct ctttctattt gttatataaa gttattatat aactttttga ttgatttttt
   143461 acaaaaaaac attagtaaac tgctaataat ttatactaag atttatttca aatatttata
   143521 gatagtcttt ttctatagtt aagttatcca ttaaattaca tagtagtata tccattttag
   143581 ttgatatatt ttcagctacc tcccttgaat attattacaa taagtctaaa tcttctttct
   143641 tccataaata tattatgagg aaagactatc atctttaatg gtttaatagt ataaaagata
   143701 ttaatttaaa aaaatctatt tttatatcag atgggtattg ttttttataa aactgaaaaa
   143761 atcacctaat agaatctatg attttttaac ataacctcca taaattagta ccaattatca
   143821 cttcaaattc aaatttttat ctgaagaata tacaatcata tttccatact caacataata
   143881 ttgagtgttt ggcttgtatt tacaaattat tattctaaca aaaacaacta tacatagatt
   143941 aaaatcacct tctaaacata attattaaaa tttaattaat gatatcaatt ccattaatct
   144001 cttttaataa aaaattctct tttctatatg aaaattattt caaaactttt aagttaaata
   144061 caaaaatgac tataaataga tttttattta cacaaatcta tttatagtca ctatagttta
   144121 tccaataaaa taataaaata aatatgtaca caataaatgt tatttacgtc ttaagtattt
   144181 atttgttaga tttttaatta aaaaaaacaa gctaatgaac attaatgcta aatctagcat
   144241 tatattactt tgatctcctg tttgtacatt ttgacctaca ttactatcta aactttcatc
   144301 tgtatctata tttatatctg gttttccatt cccatctgta tctatattta tatccggctt
   144361 tccatcccca tctgtatcta tatttatatc cggttttcca tctccatcta tatctatatt
   144421 tatatctggt tttccatctc catctatatc tatatttata tctggttttc catctccatc
   144481 tatatctata tttatatctg gttttccatc tccatctgta tctatattta tatctggttt
   144541 tccatctcca tctatatcta tatttatatt cggttttcca tccccatctg tatctatatc
   144601 tggtttgcta tttcctccat tatctgggtc tggtttacta tttccaccgt tatctggatc
   144661 cggtttgcta tttcctccat tatctggatc tggtttacca ttgccaccgt tatctggatc
   144721 tggtttgcta tttcctccgt tatctggatc
//
LOCUS       DAMJWU010000002       137833 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMJWU010000002 DAMJWU010000000
VERSION     DAMJWU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 137833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 137833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..137833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.002"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            71..187
                     /gene="rrf"
                     /locus_tag="PC460_000113"
     rRNA            71..187
                     /gene="rrf"
                     /locus_tag="PC460_000113"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            307..498
                     /locus_tag="PC460_000114"
     CDS             307..498
                     /locus_tag="PC460_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF2599023.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            653..2062
                     /locus_tag="PC460_000115"
     CDS             653..2062
                     /locus_tag="PC460_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2599024.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2193..2882
                     /locus_tag="PC460_000116"
     CDS             2193..2882
                     /locus_tag="PC460_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF2599025.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2885..3820
                     /locus_tag="PC460_000117"
     CDS             2885..3820
                     /locus_tag="PC460_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF2599026.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMNGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3817..4710
                     /locus_tag="PC460_000118"
     CDS             3817..4710
                     /locus_tag="PC460_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF2599027.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4788..5534
                     /locus_tag="PC460_000119"
     CDS             4788..5534
                     /locus_tag="PC460_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF2599028.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5535..6368
                     /gene="rsmI"
                     /locus_tag="PC460_000120"
     CDS             5535..6368
                     /gene="rsmI"
                     /locus_tag="PC460_000120"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF2599029.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFIFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6391..7095
                     /locus_tag="PC460_000121"
     CDS             6391..7095
                     /locus_tag="PC460_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF2599030.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7283..7711)
                     /locus_tag="PC460_000122"
     CDS             complement(7283..7711)
                     /locus_tag="PC460_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2599031.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7748..9178)
                     /locus_tag="PC460_000123"
     CDS             complement(7748..9178)
                     /locus_tag="PC460_000123"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF2599032.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9310..9894
                     /locus_tag="PC460_000124"
     CDS             9310..9894
                     /locus_tag="PC460_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF2599033.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9910..10431
                     /locus_tag="PC460_000125"
     CDS             9910..10431
                     /locus_tag="PC460_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HDF2599034.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10497..10743
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10827..12764
                     /gene="metG"
                     /locus_tag="PC460_000126"
     CDS             10827..12764
                     /gene="metG"
                     /locus_tag="PC460_000126"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF2599035.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12794..13564
                     /locus_tag="PC460_000127"
     CDS             12794..13564
                     /locus_tag="PC460_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF2599036.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13901..14332
                     /gene="phnG"
                     /locus_tag="PC460_000128"
     CDS             13901..14332
                     /gene="phnG"
                     /locus_tag="PC460_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF2599037.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14347..14922
                     /gene="phnH"
                     /locus_tag="PC460_000129"
     CDS             14347..14922
                     /gene="phnH"
                     /locus_tag="PC460_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF2599038.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14925..16010
                     /locus_tag="PC460_000130"
     CDS             14925..16010
                     /locus_tag="PC460_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF2599039.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            16030..16899
                     /locus_tag="PC460_000131"
     CDS             16030..16899
                     /locus_tag="PC460_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF2599040.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16892..17758
                     /locus_tag="PC460_000132"
     CDS             16892..17758
                     /locus_tag="PC460_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2599041.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17799..18512
                     /locus_tag="PC460_000133"
     CDS             17799..18512
                     /locus_tag="PC460_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2599042.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18513..19682
                     /locus_tag="PC460_000134"
     CDS             18513..19682
                     /locus_tag="PC460_000134"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF2599043.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19686..20522
                     /locus_tag="PC460_000135"
     CDS             19686..20522
                     /locus_tag="PC460_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2599044.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20523..21518
                     /locus_tag="PC460_000136"
     CDS             20523..21518
                     /locus_tag="PC460_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2599045.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21536..23224
                     /locus_tag="PC460_000137"
     CDS             21536..23224
                     /locus_tag="PC460_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2599046.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGTSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23236..24303
                     /locus_tag="PC460_000138"
     CDS             23236..24303
                     /locus_tag="PC460_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2599047.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDVMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24740..25735
                     /locus_tag="PC460_000139"
     CDS             24740..25735
                     /locus_tag="PC460_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2599048.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            25753..27441
                     /locus_tag="PC460_000140"
     CDS             25753..27441
                     /locus_tag="PC460_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2599049.1"
                     /translation="MITIKNKRRISDSKIQKCFLVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            27453..28520
                     /locus_tag="PC460_000141"
     CDS             27453..28520
                     /locus_tag="PC460_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2599050.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            28712..29242
                     /gene="rnmV"
                     /locus_tag="PC460_000142"
     CDS             28712..29242
                     /gene="rnmV"
                     /locus_tag="PC460_000142"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF2599051.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            29413..30282
                     /gene="rsmA"
                     /locus_tag="PC460_000143"
     CDS             29413..30282
                     /gene="rsmA"
                     /locus_tag="PC460_000143"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF2599052.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            30348..31613
                     /locus_tag="PC460_000144"
     CDS             30348..31613
                     /locus_tag="PC460_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599053.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEGSEE
                     EYIIEEYDEDEYEDYWEDDEDIDEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQ
                     GESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYL
                     GYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGF
                     LRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            31764..32879
                     /locus_tag="PC460_000145"
     CDS             31764..32879
                     /locus_tag="PC460_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2599054.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            33065..33961
                     /locus_tag="PC460_000146"
     CDS             33065..33961
                     /locus_tag="PC460_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2599055.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            34031..34828
                     /locus_tag="PC460_000147"
     CDS             34031..34828
                     /locus_tag="PC460_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599056.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(34883..36235)
                     /locus_tag="PC460_000148"
     CDS             complement(34883..36235)
                     /locus_tag="PC460_000148"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF2599057.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            36454..37332
                     /gene="purR"
                     /locus_tag="PC460_000149"
     CDS             36454..37332
                     /gene="purR"
                     /locus_tag="PC460_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF2599058.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            37488..37766
                     /gene="spoVG"
                     /locus_tag="PC460_000150"
     CDS             37488..37766
                     /gene="spoVG"
                     /locus_tag="PC460_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF2599059.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            37979..39358
                     /gene="glmU"
                     /locus_tag="PC460_000151"
     CDS             37979..39358
                     /gene="glmU"
                     /locus_tag="PC460_000151"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF2599060.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            39453..40403
                     /locus_tag="PC460_000152"
     CDS             39453..40403
                     /locus_tag="PC460_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF2599061.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      41247..41332
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            41494..42015
                     /locus_tag="PC460_000153"
     CDS             41494..42015
                     /locus_tag="PC460_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2599062.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTTTDKAGLTILETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTDTEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDELTDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            42167..43843
                     /locus_tag="PC460_000154"
     CDS             42167..43843
                     /locus_tag="PC460_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2599063.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            43855..45063
                     /locus_tag="PC460_000155"
     CDS             43855..45063
                     /locus_tag="PC460_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2599064.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            45078..46790
                     /locus_tag="PC460_000156"
     CDS             45078..46790
                     /locus_tag="PC460_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF2599065.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            46783..47691
                     /gene="pilO"
                     /locus_tag="PC460_000157"
     CDS             46783..47691
                     /gene="pilO"
                     /locus_tag="PC460_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF2599066.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            47718..48284
                     /locus_tag="PC460_000158"
     CDS             47718..48284
                     /locus_tag="PC460_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2599067.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            48321..48848
                     /locus_tag="PC460_000159"
     CDS             48321..48848
                     /locus_tag="PC460_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2599068.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            48869..50407
                     /locus_tag="PC460_000160"
     CDS             48869..50407
                     /locus_tag="PC460_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599069.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRNSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            50507..51589
                     /locus_tag="PC460_000161"
     CDS             50507..51589
                     /locus_tag="PC460_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF2599070.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            51570..52352
                     /locus_tag="PC460_000162"
     CDS             51570..52352
                     /locus_tag="PC460_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2599071.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILSGKQLLELYFSMVI"
     gene            52454..53104
                     /locus_tag="PC460_000163"
     CDS             52454..53104
                     /locus_tag="PC460_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2599072.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            53118..53678
                     /locus_tag="PC460_000164"
     CDS             53118..53678
                     /locus_tag="PC460_000164"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF2599073.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            53695..57081
                     /gene="mfd"
                     /locus_tag="PC460_000165"
     CDS             53695..57081
                     /gene="mfd"
                     /locus_tag="PC460_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF2599074.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            57112..58107
                     /locus_tag="PC460_000166"
     CDS             57112..58107
                     /locus_tag="PC460_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2599075.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            58231..58770
                     /gene="spoVT"
                     /locus_tag="PC460_000167"
     CDS             58231..58770
                     /gene="spoVT"
                     /locus_tag="PC460_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF2599076.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            58890..60503
                     /locus_tag="PC460_000168"
     CDS             58890..60503
                     /locus_tag="PC460_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2599077.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            60610..62058
                     /gene="mazG"
                     /locus_tag="PC460_000169"
     CDS             60610..62058
                     /gene="mazG"
                     /locus_tag="PC460_000169"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF2599078.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            62147..62425
                     /locus_tag="PC460_000170"
     CDS             62147..62425
                     /locus_tag="PC460_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2599079.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            62497..62739
                     /locus_tag="PC460_000171"
     CDS             62497..62739
                     /locus_tag="PC460_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF2599080.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYKILE"
     gene            62801..63055
                     /locus_tag="PC460_000172"
     CDS             62801..63055
                     /locus_tag="PC460_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF2599081.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            63056..63589
                     /locus_tag="PC460_000173"
     CDS             63056..63589
                     /locus_tag="PC460_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599082.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            63595..63879
                     /locus_tag="PC460_000174"
     CDS             63595..63879
                     /locus_tag="PC460_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF2599083.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            63992..64912
                     /locus_tag="PC460_000175"
     CDS             63992..64912
                     /locus_tag="PC460_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF2599084.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            65002..67374
                     /gene="spoIIE"
                     /locus_tag="PC460_000176"
     CDS             65002..67374
                     /gene="spoIIE"
                     /locus_tag="PC460_000176"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF2599085.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            67725..69617
                     /gene="pepF"
                     /locus_tag="PC460_000177"
     CDS             67725..69617
                     /gene="pepF"
                     /locus_tag="PC460_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2599086.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLEADNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            69731..70696
                     /gene="galU"
                     /locus_tag="PC460_000178"
     CDS             69731..70696
                     /gene="galU"
                     /locus_tag="PC460_000178"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2599087.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            71196..72800
                     /gene="rho"
                     /locus_tag="PC460_000179"
     CDS             71196..72800
                     /gene="rho"
                     /locus_tag="PC460_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF2599088.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            72953..73153
                     /gene="rpmE"
                     /locus_tag="PC460_000180"
     CDS             72953..73153
                     /gene="rpmE"
                     /locus_tag="PC460_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF2599089.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            73298..74191
                     /locus_tag="PC460_000181"
     CDS             73298..74191
                     /locus_tag="PC460_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF2599090.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            74209..75057
                     /gene="prmC"
                     /locus_tag="PC460_000182"
     CDS             74209..75057
                     /gene="prmC"
                     /locus_tag="PC460_000182"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF2599091.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            75101..76165
                     /gene="prfA"
                     /locus_tag="PC460_000183"
     CDS             75101..76165
                     /gene="prfA"
                     /locus_tag="PC460_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF2599092.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            76294..76974
                     /locus_tag="PC460_000184"
     CDS             76294..76974
                     /locus_tag="PC460_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF2599093.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            77003..78043
                     /locus_tag="PC460_000185"
     CDS             77003..78043
                     /locus_tag="PC460_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF2599094.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            78060..78509
                     /locus_tag="PC460_000186"
     CDS             78060..78509
                     /locus_tag="PC460_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF2599095.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            78526..78978
                     /gene="rpiB"
                     /locus_tag="PC460_000187"
     CDS             78526..78978
                     /gene="rpiB"
                     /locus_tag="PC460_000187"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2599096.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            79014..79643
                     /gene="upp"
                     /locus_tag="PC460_000188"
     CDS             79014..79643
                     /gene="upp"
                     /locus_tag="PC460_000188"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF2599097.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            79944..80759
                     /locus_tag="PC460_000189"
     CDS             79944..80759
                     /locus_tag="PC460_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF2599098.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            80985..81422
                     /locus_tag="PC460_000190"
     CDS             80985..81422
                     /locus_tag="PC460_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2599099.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            81607..81834
                     /locus_tag="PC460_000191"
     CDS             81607..81834
                     /locus_tag="PC460_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF2599100.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            81839..82225
                     /locus_tag="PC460_000192"
     CDS             81839..82225
                     /locus_tag="PC460_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF2599101.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            82228..82932
                     /gene="atpB"
                     /locus_tag="PC460_000193"
     CDS             82228..82932
                     /gene="atpB"
                     /locus_tag="PC460_000193"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF2599102.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            83001..83261
                     /gene="atpE"
                     /locus_tag="PC460_000194"
     CDS             83001..83261
                     /gene="atpE"
                     /locus_tag="PC460_000194"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF2599103.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            83384..83881
                     /gene="atpF"
                     /locus_tag="PC460_000195"
     CDS             83384..83881
                     /gene="atpF"
                     /locus_tag="PC460_000195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF2599104.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLVEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            83878..84423
                     /locus_tag="PC460_000196"
     CDS             83878..84423
                     /locus_tag="PC460_000196"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF2599105.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            84440..85942
                     /locus_tag="PC460_000197"
     CDS             84440..85942
                     /locus_tag="PC460_000197"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF2599106.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            85970..86848
                     /gene="atpG"
                     /locus_tag="PC460_000198"
     CDS             85970..86848
                     /gene="atpG"
                     /locus_tag="PC460_000198"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF2599107.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            86861..88255
                     /gene="atpD"
                     /locus_tag="PC460_000199"
     CDS             86861..88255
                     /gene="atpD"
                     /locus_tag="PC460_000199"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF2599108.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            88258..88518
                     /gene="atpC"
                     /locus_tag="PC460_000200"
     CDS             88258..88518
                     /gene="atpC"
                     /locus_tag="PC460_000200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF2599109.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            88980..89360
                     /locus_tag="PC460_000201"
     CDS             88980..89360
                     /locus_tag="PC460_000201"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF2599110.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRDEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            89375..89836
                     /locus_tag="PC460_000202"
     CDS             89375..89836
                     /locus_tag="PC460_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2599111.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            89857..90447
                     /locus_tag="PC460_000203"
     CDS             89857..90447
                     /locus_tag="PC460_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599112.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            90462..91619
                     /gene="alr"
                     /locus_tag="PC460_000204"
     CDS             90462..91619
                     /gene="alr"
                     /locus_tag="PC460_000204"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2599113.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            91652..91939
                     /locus_tag="PC460_000205"
     CDS             91652..91939
                     /locus_tag="PC460_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF2599114.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            91908..92291
                     /locus_tag="PC460_000206"
     CDS             91908..92291
                     /locus_tag="PC460_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF2599115.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            92462..93289
                     /locus_tag="PC460_000207"
     CDS             92462..93289
                     /locus_tag="PC460_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2599116.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            93282..94223
                     /locus_tag="PC460_000208"
     CDS             93282..94223
                     /locus_tag="PC460_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2599117.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            94646..95740
                     /locus_tag="PC460_000209"
     CDS             94646..95740
                     /locus_tag="PC460_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599118.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            95966..96382
                     /locus_tag="PC460_000210"
     CDS             95966..96382
                     /locus_tag="PC460_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599119.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(96564..97133)
                     /locus_tag="PC460_000211"
     CDS             complement(96564..97133)
                     /locus_tag="PC460_000211"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF2599120.1"
                     /translation="MKKEFRKKVIESRKNKNANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            97287..98444
                     /locus_tag="PC460_000212"
     CDS             97287..98444
                     /locus_tag="PC460_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF2599121.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            99048..99530
                     /locus_tag="PC460_000213"
     CDS             99048..99530
                     /locus_tag="PC460_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2599122.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF
                     "
     gene            complement(100229..101383)
                     /gene="nagA"
                     /locus_tag="PC460_000214"
     CDS             complement(100229..101383)
                     /gene="nagA"
                     /locus_tag="PC460_000214"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2599123.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYIPALSCNIDNVCGSIHQGMEADFIVTDKDFNIYETYISGICKYKVNK"
     gene            complement(101466..102191)
                     /locus_tag="PC460_000215"
     CDS             complement(101466..102191)
                     /locus_tag="PC460_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2599124.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            102458..103387
                     /gene="pfkB"
                     /locus_tag="PC460_000216"
     CDS             102458..103387
                     /gene="pfkB"
                     /locus_tag="PC460_000216"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2599125.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVSK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYYVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(103516..104433)
                     /locus_tag="PC460_000217"
     CDS             complement(103516..104433)
                     /locus_tag="PC460_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2599126.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMQFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAISGEECIKAQRLLEKIINSVK"
     gene            104903..106072
                     /locus_tag="PC460_000218"
     CDS             104903..106072
                     /locus_tag="PC460_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2599127.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIVEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            106121..106972
                     /gene="gatY"
                     /locus_tag="PC460_000219"
     CDS             106121..106972
                     /gene="gatY"
                     /locus_tag="PC460_000219"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2599128.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            107298..109199
                     /locus_tag="PC460_000220"
     CDS             107298..109199
                     /locus_tag="PC460_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2599129.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            109202..110245
                     /gene="ypdE"
                     /locus_tag="PC460_000221"
     CDS             109202..110245
                     /gene="ypdE"
                     /locus_tag="PC460_000221"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2599130.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            110260..110565
                     /locus_tag="PC460_000222"
     CDS             110260..110565
                     /locus_tag="PC460_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2599131.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            110662..110997
                     /locus_tag="PC460_000223"
     CDS             110662..110997
                     /locus_tag="PC460_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2599132.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            111029..112351
                     /locus_tag="PC460_000224"
     CDS             111029..112351
                     /locus_tag="PC460_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2599133.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            112361..113431
                     /locus_tag="PC460_000225"
     CDS             112361..113431
                     /locus_tag="PC460_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2599134.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            113436..114623
                     /locus_tag="PC460_000226"
     CDS             113436..114623
                     /locus_tag="PC460_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2599135.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(114820..115692)
                     /locus_tag="PC460_000227"
     CDS             complement(114820..115692)
                     /locus_tag="PC460_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF2599136.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(116086..116277)
                     /locus_tag="PC460_000228"
     CDS             complement(116086..116277)
                     /locus_tag="PC460_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599137.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            116497..117543
                     /gene="cobT"
                     /locus_tag="PC460_000229"
     CDS             116497..117543
                     /gene="cobT"
                     /locus_tag="PC460_000229"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF2599138.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            117575..118135
                     /gene="cobU"
                     /locus_tag="PC460_000230"
     CDS             117575..118135
                     /gene="cobU"
                     /locus_tag="PC460_000230"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF2599139.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            118145..118918
                     /gene="cobS"
                     /locus_tag="PC460_000231"
     CDS             118145..118918
                     /gene="cobS"
                     /locus_tag="PC460_000231"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF2599140.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            118938..119552
                     /locus_tag="PC460_000232"
     CDS             118938..119552
                     /locus_tag="PC460_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2599141.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      119619..119798
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            119928..121427
                     /locus_tag="PC460_000233"
     CDS             119928..121427
                     /locus_tag="PC460_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF2599142.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            121466..122842
                     /locus_tag="PC460_000234"
     CDS             121466..122842
                     /locus_tag="PC460_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF2599143.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            122843..123814
                     /locus_tag="PC460_000235"
     CDS             122843..123814
                     /locus_tag="PC460_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2599144.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            123846..124916
                     /locus_tag="PC460_000236"
     CDS             123846..124916
                     /locus_tag="PC460_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2599145.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEEREYML
                     QELRNIRNIKVYDTDANFILIKIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            124918..125805
                     /locus_tag="PC460_000237"
     CDS             124918..125805
                     /locus_tag="PC460_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2599146.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            125793..126425
                     /locus_tag="PC460_000238"
     CDS             125793..126425
                     /locus_tag="PC460_000238"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF2599147.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            126434..127660
                     /locus_tag="PC460_000239"
     CDS             126434..127660
                     /locus_tag="PC460_000239"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF2599148.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            127657..128265
                     /locus_tag="PC460_000240"
     CDS             127657..128265
                     /locus_tag="PC460_000240"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF2599149.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            128255..128830
                     /locus_tag="PC460_000241"
     CDS             128255..128830
                     /locus_tag="PC460_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF2599150.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            128823..129575
                     /locus_tag="PC460_000242"
     CDS             128823..129575
                     /locus_tag="PC460_000242"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF2599151.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            129559..130680
                     /gene="cbiG"
                     /locus_tag="PC460_000243"
     CDS             129559..130680
                     /gene="cbiG"
                     /locus_tag="PC460_000243"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF2599152.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYHVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            130677..131402
                     /gene="cobJ"
                     /locus_tag="PC460_000244"
     CDS             130677..131402
                     /gene="cobJ"
                     /locus_tag="PC460_000244"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF2599153.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            131399..132151
                     /gene="cobK"
                     /locus_tag="PC460_000245"
     CDS             131399..132151
                     /gene="cobK"
                     /locus_tag="PC460_000245"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF2599154.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            132170..133648
                     /locus_tag="PC460_000246"
     CDS             132170..133648
                     /locus_tag="PC460_000246"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF2599155.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            133678..134583
                     /gene="hemC"
                     /locus_tag="PC460_000247"
     CDS             133678..134583
                     /gene="hemC"
                     /locus_tag="PC460_000247"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF2599156.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSAIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            134573..136072
                     /gene="cobA"
                     /locus_tag="PC460_000248"
     CDS             134573..136072
                     /gene="cobA"
                     /locus_tag="PC460_000248"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF2599157.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            136089..137054
                     /gene="hemB"
                     /locus_tag="PC460_000249"
     CDS             136089..137054
                     /gene="hemB"
                     /locus_tag="PC460_000249"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF2599158.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            137207..137785
                     /locus_tag="PC460_000250"
     CDS             137207..137785
                     /locus_tag="PC460_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF2599159.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        55151 a        14769 c        24992 g        42921 t
ORIGIN      
        1 aaagaaacta gtattactag gttctataaa cttattaaaa acttaataga aatattaagc
       61 ataaagatta tgtggttaca atagcagaga ggatacacct gttcccattc cgaacacaga
      121 agttaagctc tctagcgctg atggtacttg gtgggaaact gcctgggaga gtaggacgta
      181 gccacgtaat tttttttatt ttttgtcaaa aaaaacaaaa aatacgtatt tcttttttca
      241 tttcgggaaa actaaacaca ggaattatgg actttataaa aatttaattc ttgaggagtg
      301 atataagtga aaaaagttgc tttaatttta gctataatag gtgcgcttaa ctggggtgca
      361 attggtattc taggtacaga cttaataggt agtatctttg gtggaacata tgaaatggta
      421 agtagaataa tatacttcat tgtaggttta gctggattat acttaatacc aagtttaatg
      481 tctgatgata gagaataatt tatgctttta tgttaaatac ctcattctaa atgtttagaa
      541 tgaggtattt aattttataa aaatattaat ttgtttcttt tttaatggta aaatagataa
      601 gaattgaagt tttaaaaaga agtattatgt aaaaaaggag tactattaat atatgtttat
      661 tattaatgaa ttaaataaga ttgtaaatga agaagtaata tcttttcata tgcctggtca
      721 taaaaaggga caggtttata aaaagttagg atataaagat atactaaaaa atttgtataa
      781 aatggataca actgagatat taggaacaga taatttacac tttccggaag gaattataaa
      841 aaattcacaa aaaaaagcat caagagtatt taaaagtgat tatacttgct atttagtaaa
      901 tggtagtagc tgtggaatac aatctgcaat aatgtcagta tgtagtccta aaagtaaaat
      961 aataacaaat agagactgtc atcagtctgt tataaatgga tgtattttag gagatataga
     1021 tatagagtat gtgccttgtg agataagtga agagacaaat atcttaaaag ggatatctat
     1081 aagtaatgtg attgatataa tagataaaaa cttggatgca aaagcagtac tgcttactta
     1141 tcctacatat tatggaatga catatgattt agaatacata tgtaattatg cacatagcaa
     1201 aaagatggta gtgatagtag atgaagcaca tggtgcgcat ttagggctta gtgagaggct
     1261 tcctaaaaca gctcttgagc aaggtgcaga tatagttgta cagagtacac ataaaactct
     1321 tccagcattt acacaatcgt caatgataca tatacaggga gaccgagttg atatagataa
     1381 aatattgaac atgttaagaa ttacagaatc ttcaagccct tcatatttat tattatcttc
     1441 tctggaatta gcagtagata tatatgaaaa taaaggaaaa gaattaatgg aagagctttt
     1501 aaataatatt ataactttca aaaataaagt tgattcaaat attaatatat acgacacaaa
     1561 tgataaaaca aaaatattta tatcatctaa aaacatgggt ttaacaggtt atgaactaga
     1621 aaatattttg agaaataaat ataacataca agtggagtta tctaattatt atggagttct
     1681 tttaatatgt acaataggaa atactgctga ggatttctct agtttagaaa cagcattaaa
     1741 caatgttgta aaggaagact ttaaatttga aaagttagaa aataaaaaat accctgttgt
     1801 tattccaaag aaaatattaa cacctagaga agcattttat aaaactaaaa aaagtgttaa
     1861 aatatatgac agtataggta aagtttgtgg tgaatgtata gttccatacc cacctggaat
     1921 aagtgtaata tctcctggtg aagtaataag taaagaaata atagactatc taatgttttg
     1981 tcattctaaa ggcatgattg tgagtggatt gaaagatgta gatttaaatt ttatacaagt
     2041 aatagattta gaagacattt aaaaattatg atataaaagt agatagaagt agatagttta
     2101 aaattgtgaa gagaaataaa tagactgctt atatagagat atctaataaa agtactaaaa
     2161 aagtaaaaag tgtttgtatg gaggatatga aaatgaaagg taagttaata attatagaga
     2221 gtggttcaga tgcaagtgga aaggctactc agacagaaat attatatgaa agattaaaaa
     2281 aagaaggcta tatgattaaa aaggtagaat atccaaatta tgaatctcca tcatctgcct
     2341 tggtgaaaat gtacctaaaa ggagactttg gtaaaaatgc atcagatgta gacccatata
     2401 tagcatcaac tttttttaca gctgataggt atgcatcatt taaaaccgat tgggaggagt
     2461 tttacaatga aggtggaata attatcgcag atagatatac aacctctaat atggtacatc
     2521 aggcctctaa aatggatatt tgtgatagag aagagtattt aaattggtta gctgattatg
     2581 aatttaattt atttaagata ccacaacctg attgtgttat atttttagat gtgcctattg
     2641 aatttagtaa aaagcttatg gagaatagaa aaaataaaat tacaggggaa gaaaaaaagg
     2701 atattcatga aagtgacata gagtatttag agaaatctta caacaatgct ttatatatag
     2761 cagataaata tgattggaaa aaaataaatt gtgttttaga taacaaatta agaagtattg
     2821 aaagtataca taatgaaatt tataaaatag ttcttgattc aataaaacct atggagagat
     2881 aaatatgtat tttgaaaata ttatagggca agattttgca aagaaatatt taacaaattc
     2941 tataaagaaa aataaactta ataatgcgta tatgtttgaa ggaatggatg gaataggaaa
     3001 aaagaaattt gcagatgaat tatcaaaact attacttgat tatgaaaatc tcgaaaatag
     3061 tccagactat gtactcataa aaccagatgg aaatagtata aaaatagctc aaatcagaaa
     3121 tttacaatca gatatagtaa taagacctca taaagactat aaaatatata taatcaataa
     3181 tgctgaaaaa atgactgtgg aagctcaaaa tgcactttta aagacgctag aggaacctcc
     3241 aaactatgct ataataatct tagttacaaa caacaaggaa tctttattag aaactataaa
     3301 atctagatgt gatataataa agttctcacc tataccaatt gaagacttaa aacgttattt
     3361 gataaataca ggtatagaag aagaaagagc tcaattattg gctatttttt cgagaggaag
     3421 tattgagaat gctctaaatc tatctcagtc ttctgagttt tcaatgatga gagaagatat
     3481 acaacagtat attcagataa tgttagataa aaacatagtg gagattttaa atataccaaa
     3541 taatatggaa aaatatagag gtaagattat agctttatta gatatgatga taaactattt
     3601 tcgagatatc atcctattaa aagaaaatgt aaacaagaac atgttaataa atgtagataa
     3661 gctagttttc attcaaaata tgaatggaaa aattagttat tctcaattgt ccaagattat
     3721 tgatataata gaagatgcaa agagtaaaat aaaaagcaat tgcaatttca atataagcat
     3781 acaagttatg tctttaaata tatacgaggt gattaaatga taaagatagt aggtataagg
     3841 tttaaaagtg ctgggaaaat atattatttt gaccctgttg attttaatat agagcaagat
     3901 atggatgtag ttgtagaaac tgcaagaggc ttggagtatg ggaaagtagt cgttggtcca
     3961 aaagatatgg atgaaagcga attagtttct cctttgaagc caattataag aatagctaca
     4021 gaagaagata aaaaagtata tctagaaaat aaggaaaagg caaaagaaac ttttgagttg
     4081 tgccaacaaa aaataaaaga acatgagtta acaatgtttt taatagattg tgagtataca
     4141 tttgatagga ataagttgat tttttacttt acagcagaag gaagaataga ttttagagaa
     4201 ttggtaaaag acttagctgc tatatttaaa actagaatag aacttagaca aataggtgtc
     4261 agagatgaag ctaaatctat aggaggtcta gggccttgtg gtagaaaact ttgttgttca
     4321 tcttggttag gtgattttca accagtttca ataaaaatgg caaaagacca aagtctatct
     4381 ctaaatccaa caaaaatatc tggaatatgt gggagattat tctgttgctt aaaatatgag
     4441 catgatgtat atagtgaagc aatagaaaaa atgcctgttg taggtgcatt ggtgcaatca
     4501 gatgatggaa aaggaaaagt tgtagaagtg aatcctttat tagaacaaat taaagtggaa
     4561 tttcaagaca agacagtaaa gatatacaca agagaagata taaaaatact tagagagcct
     4621 aaaaaatgtg atggctgtgg tggtaaatgt aaggataatg atggattaga tgaagccaca
     4681 ctaagagagc taaagaaatt agaagattaa atttaaggta gggcttttcc tacctttttt
     4741 aaagcaatta atgcttaaag ttattaatat ttgaggagaa tttatatatg caattaaaag
     4801 attcagaaag aatagatgat ttacaattaa aaggccttaa aattatacaa gatactaatg
     4861 gtttttgttt tggtatagat gcagttttat tagccaattt tactaaaata aaaaaagatg
     4921 ccaaagtagt agatttgggt actggaactg gcattatacc tattttgata gctggaaaaa
     4981 gtgaagctaa aaaaattatt ggcgtggaaa tacaagaaga tgtatatgaa atggcaactc
     5041 gttctataaa attaaatgga ttagaagaaa gagttgagat tataaatgaa gatataaaat
     5101 acatagacaa agtcttagac ataaatggtt accatgtagt cacatcaaat cctccatata
     5161 tgcatattga tggaataaaa aatccaaatg ataaaaaagc aatatcaaga catgaagtaa
     5221 ggtgcaattt ggaagatgta attagagctg catccagatt ggttatgcct agaggaaaat
     5281 tcttcatgat acatagacca acaagattgg ttgatataat aactttaggg agaaaatata
     5341 aattagaacc aaaggttatt caatttgtgc atccaagacc tcaaaaagca ccgaacttag
     5401 ttttggttca atttgtaaaa gatggaaggc cagagcttaa aatattggac ccattgtatg
     5461 tttatggaga agatgggaat tatacaaaag aattaaaagc aatatataat aataaagata
     5521 taggagagtt gtaaatgagc gggaaattgt acatatgtcc aacaccaata ggaaacctag
     5581 aagatataac atatagaact ttaagaatat taaaagaagt agacttgatt gcagcagaag
     5641 atacaagaca tagtataaaa cttttaaatc attttgaaat ctcaaaacca ctaactagtt
     5701 atcatgaaca taataaggat tcaaagggag attatttgat aaataaacta attgatggtg
     5761 aaaatatagc tctgataagt gatgcaggta tgcctggtat atctgaccca ggagaagaga
     5821 taataaaaca agctatacaa aataacatag agatagaagt attgccaggt gcaacagcct
     5881 ttgtaacggc tttagttggc tctggtatgg acacacatag atttattttt gagggctttt
     5941 tagatagaga taaaaaagtt aggaaagcac aattggaaga attaaaggaa gaaagtagaa
     6001 ctataatttt ctatgaatca ccacatagat tgaaggatac actaaaagat atgcttaaaa
     6061 ttcttggtaa taggaatatt tctataaata gagaacttac aaaaaaatat caagaaataa
     6121 taagagaaaa tatagaaact accataaaaa tatttgagga aaaagaagta agaggagagt
     6181 ttgtattaat agtagagggt tttcatggtg agaagtcaaa gaaagaaaat tatgatgggc
     6241 ttacagatag ggaatatgtt ttaaaactaa ttgaaagtgg tatgaacaaa aaagatgcaa
     6301 taaaaatagt ttgtaaagat agaaagttaa agaaagatgt ggtttataaa caagttttag
     6361 atttataaaa cctgaatctg aggtgatttt atggaaatag ggaaaattat aatagagagt
     6421 ttaaagaggg aagatacaca taaaatattt atagaattag ataataagca tgaattagaa
     6481 aaaataattc ctaaaataag ctgtatgaag tctgtaggtg aatgtaaata tcatgtagta
     6541 aattgttttg aacattctat aaatgcatta aaagaattag aaatagtatt aaatgataag
     6601 gatttttttc caatacatct aagaaaacat gtgtcaaact atttaaaaac atatattgaa
     6661 gaaggaataa ataagttaca tgtattaaag ctgggaacat ttttacatga tataggtaag
     6721 cctgacagta tgaccttaga tgaaaccgga agagttcatt tcacaaatca tgaaaaaata
     6781 ggtgctcaaa taattgacaa tatgggaata aagttagagt tatcaacaga gacatataga
     6841 cttataagta aatatgtaag atatcatatg atacttttgt ctctatataa gaaaaatgat
     6901 ttgagtagga aagaattaat taatgtattt aacttagtag atgaggacac tataggtgta
     6961 ataatgcttg ggtatgcaga tatagtgtct acagtaaaat tattgggcaa gagtggagaa
     7021 gtcagtgtgt taaagacata tatggaatat attttaacaa attacttgta taagagtaat
     7081 tatacacatg tataaattta gttaatataa ttgagtttta gatgattaat tgaagtttga
     7141 aatatacgga tttcaaaaac ataaaagaag ttgtctttgt tgtaaaatta tgaaagacaa
     7201 ctttttttat atcaaaaatt gaaattttat taaggatact taatggtcta ttaattaata
     7261 aaccattagt atttaattta tattacaata ttgattttgc ttgtattggc gttgatagta
     7321 taacagaggt cttagtagaa ccaattttct ttatagtatc aatcacattt tctagctcgt
     7381 acatatcttt tacaattact tttaaaagta agcaatcatc acctgttata tggtgacatt
     7441 ctacaatcct agggtccttt gcagctgact caataaattc tgtatatcca ttgcttggaa
     7501 gagaaatatg aataaatgcc tttataactc tgcctaatga atctgggttg acaatagctt
     7561 tatatccttc tataacacca gactcttcta atcttttgac tctttctgaa actgcaggag
     7621 aagttaaacc aactattttt cctaagtcct tcatagaaat tctcccatca tcctgtaaaa
     7681 tttctatgat tctgtaatct gtaacatcca taaataaaca gtccccctta aataaaaata
     7741 attaatgcta taaaactttt ttagaatcta tcatttgtgt ttttaaatac caaagatttc
     7801 tagataaatc aaccttataa atgtgtggtt ttgaaagtct tttgtattgt tcccagatag
     7861 tacttaattc atattgtaca tgatttttta tttcaagaac agctttttta ttatatttgc
     7921 atctgccttt tatatacaat ggtttaagaa gctctttaac tttgtaattt gtaaatacct
     7981 tcgttttcca tgtataagta ggatggaaga tttctaaagg tttagactca tctataactt
     8041 catcatgtaa cataatcaaa tctgcctctg ccttattgtc ttcgttatat attcttataa
     8101 ctttcttgta accaggatta tttatttttt ctggattctc agaaatcttt atcttaggtt
     8161 caagaactcc tttttcacaa gatgcagcta atttataaac tcctccaaga gaaggtgaat
     8221 cgaaagatgt aatgagcttt gtacctactc cccatgaatt tatagtagct ccttctgctt
     8281 taagtgatgt tatggtatat tcgtctaggt cattagatgc agttatgctg atatctgaaa
     8341 agccagcatt atctaattgt tttttagctt ctttagataa ataagctaaa tctccagagt
     8401 caagtcttat acccataggt ttgtatcctt tttcagaaat atttttgaat acttttattg
     8461 cattaggaac tccactgttt aatacatcat aagtatctac aagtagtagg catttatctg
     8521 gataaacatc agcatatgct tggaatgctt caagttcatt atcaaatttt tgtacccaac
     8581 tgtgagcttg tgtaccaatt ataggtatat caaacatttt ccctgctagt acgtttgcag
     8641 ttccagcaca accaccaact acggcagccc tagccccata tatccctgca tctggacctt
     8701 gtgcacgacg taatccaaat tcaaatacag ggtctccttg agctgcaaag caaactctag
     8761 atgctttagt agcaataaga gactgaaaat taactattgt taacaatgct gtttcaataa
     8821 gttgagcttg atataaaggt gccttaacag taattaatgg ctcattaggg aacatgatag
     8881 ttccttcctc aacagcatat atatctcctg tgaatttaaa ttcttttaaa aattttaaga
     8941 acttgtcaga aaataaattt aagtttttta agtactctaa atcttcatct gaaaaatgaa
     9001 gattatctat gtattcaaca acctgatcaa taccacaaac tatagtatac cctccatcac
     9061 aagcgttttt tctaaaaaac atatcaaata caacaatgtc ttcatgaata ttcttttcaa
     9121 aatagccatt taacatagta agctgatata aatcagtaag aagtgttaga tttctcatta
     9181 tttctctcct taattattat taaatattaa aaattgtaaa aactcccaca cttatactaa
     9241 aacataatat agtacaactt tcaagtataa agtattacct aactatcata aaattattta
     9301 aagaggagga tgattataat aggaaaaata tggttttata tggtatcgat aggaataata
     9361 ggaagcttga tttctaaaaa tatgggtgaa ttaaataaag ttatattaag tgaaacatca
     9421 aaaggtatag agtttgcaat aagtctagct ggtatcatgg cactctggat ggggataatg
     9481 aatatcgcaa aagaatcagg tttaattgaa aagataggtc aaaaactaaa tcctataatg
     9541 agaagattat ttccttctat tccaaaaggt cataaagcaa tgtcttatat agttatgaat
     9601 attgctttaa atatgttggg tgctggaaat ggtgcaacag catttggttt aaaagcaatg
     9661 agtgagcttc aaactttgaa taacaagaaa gatacagcca ctaatgatat gattatgttt
     9721 atggtaatca atatatcatc tatacaaata attccattta ctataatcaa actaaggatg
     9781 gatatggggg ctcaaaatcc aagtgaaata atttttacga ctttatttgc aaccataata
     9841 tctacagcag tagcaataat aacatgtaaa ttttttcaaa gaagatatag ataaagggga
     9901 tgtagataca tggagatatt ttcaaactta cttataccaa taataattct gtacatagta
     9961 gtttatggta aatataaaaa aatagatgtg tatgatagtt ttgtaaaagg agcaattgat
    10021 gggttaaaag cagcatggga tatcctacca tatataatag gtatattttt ggcaatagga
    10081 atattcaaaa ctggtaaagg tcttgatatg ttagaatggc tatttacacc aatagcaaat
    10141 atgatgagta taccaaaaga attaataggt ctaataagtg taaaaccttt atctggaagt
    10201 ggtgctttag ggatgtacag tgaacttgca aatagagttg gaataggaag tttagtagaa
    10261 aaaatgggtg ctacgattgt aggttcttct gagactattt tttacacaat ggcaatatac
    10321 tatggaagtc tgaagataaa aaatactaga catacattat catgtgctat gattagccat
    10381 gttgcaggtg tcatagcagc tgtttttatt tgctatgtaa tatttgtata atttgttgac
    10441 aatatgggaa aaattttata atatagtgat tatatagaaa caaatagtaa tacaaattac
    10501 aagctatgaa aagaagagta aataaaggtt ttttctatca gagagctagg ttaggtgaaa
    10561 gctagtgtca aaagctttat tgaagatggt cttggagctt tttatctgag caagtgtgct
    10621 agggtaaaac ggttggacac gttatagtct actaaggtat ctataaaatg tttctacatt
    10681 tcatgtgaaa cataactatt atagatgaat tagggtggta ccgtgaataa tctcgcccct
    10741 atgttaactc ataggaggcg attttttatt atttaaatga aagaatcata tgattacgaa
    10801 cgataaaaaa ttaggaggta aataacatga gcaaaccgag tttttatgta acaacaccaa
    10861 tatactatcc aagtggagga ttacacatag gacatactta ttcaacagta gctgcagata
    10921 caatagctag attcaagcgt ttctgtggat atgatgtaaa gttcttaaca ggaacagatg
    10981 agcatggtga aaaaattcaa aagaaagcca tagaacaagg aatgtcagag atagagtatc
    11041 ttgatggaat gataaaagat ataaaggcct tatggaatac tatggatata tcttatgatg
    11101 attttataag aactacagaa aaaagacata cagacataat acaaaagata tttactaaac
    11161 tatatgagca aggtgatata tataaaggtg aatatgaagg tagatactgt actccttgtg
    11221 agagtttctg gacagaatct caattactag aaggaaataa atgccctgac tgtggaagag
    11281 aaacatattt agtaaaagaa gaatcttatt tctttagatt atctaaatat gaagacagat
    11341 taaaagagtt atttaaagat gatagtttct gttttcctgc tgctagaaaa aatgaaatgg
    11401 tggcaaactt tttagacaaa ggattagaag atttaagcgt aactagaacg acttttgact
    11461 ggggtataaa agttcctttt gatgaaaaac atgtaattta tgtgtgggta gatgctttat
    11521 gtaactatat aacagcatta ggttatatga ctgataatga tgaagaattt aaaaaatatt
    11581 ggcctgccaa tgttcaaata gtaggtaaag aaatagtgag attccataca ataatatggc
    11641 cagcgctttt aatggcttta ggtctagaag ttccaaaaca agtatttgga catggatgga
    11701 tactatttgc tgatgataaa atgagtaaat caaaaggtaa tgtggtttat ccagaaccta
    11761 taatagaaag atatggaata gacacactta agtacttctt attaagagag tttgcatttg
    11821 ggcaagatgg aagttatact catagaaatt ttgtaacgag aataaattat gatttagcaa
    11881 atgatttagg aaacttaata agtagaactg tagctatggt tgaaaaatac aataatggta
    11941 taataccaac agcaaaagtt tcaactaatt ttgatgctga cttaaaagaa caagctgttt
    12001 caactagaga aaactttgaa gctgaaatgg ataaaatgca attccatgaa gcattagaga
    12061 gtgtatggaa attagttaga agaactaaca aatatataga tgaaactatg ccttgggcac
    12121 tagctaaaga tgaaactaag aaagatgaat tagacacagt tttatataat ttatgtgaat
    12181 ctataagaat aatcgcaaca ctaataaatc ctataatgaa tgagactgct aataaaatat
    12241 atgaacatat aggaataaaa ggtcaagatg atataactac atgggaaagt acaaaaacat
    12301 ttggtcttat tggagaaaat gtaaaagtat ttaaaggtga acctttattc ccaagattag
    12361 atgtagaaaa agagatagaa gaattaacta aaatgttctc tgggaaacct ccagtagaag
    12421 aaaaaccttt agagcataaa gaagaaatta ctattgatga tttagacaaa atagagctta
    12481 gagtagggaa aataataagc tgtgaaaaac atccaaaagc gaataaatta ttagtatcac
    12541 aagttaagat tggaccagaa acaagacaaa tagtatcagg aatagctgaa tactataagc
    12601 cagaggattt agtaggaaaa gaggttacag tggtatgtaa cttaaaacca gttaaattaa
    12661 gaggtgtaga atctcaaggt atgatattag cagcaggtga tgatggagac ccatatgttt
    12721 taccatttac acaaggggct aaagatggat gtgaggttcg ttagtaggta attaaatagt
    12781 aaggagatta aagatgttat ttgactcaca tgcacattta aatgatgaaa gttttgatga
    12841 agatagagat gaacttattg gttcattaaa agataaaggt gtagatttgg ttgtaaatcc
    12901 aggagcagat atagagacgt cgataacagc aattgaactt gcgaaaaaat acgactttat
    12961 atattcagca gttggggtac atccacatga tgtatcaaaa ctagatgata cagcaataga
    13021 aactctaaga aaacttgcaa ctgagaatga aaaagttgta gctattggag agattggatt
    13081 agattattat tatgattatt ctccaagaga agaacaaaaa gagtggttta agaagcaaat
    13141 tgaattagct aatgagctaa aacttccaat aataatacat gatagagatg cacatggaga
    13201 tacttttgag ataataaaga atactaaaaa tcctgaaata ggatgtgtac ttcattgtta
    13261 tagtgggaat gtagagttag ctagagaata tgttaagatg ggttgttata tatcaattcc
    13321 aggtacggtt acttttaaga ataataaaaa aactagagaa gtagtaagag aaatacctct
    13381 agaaagatta ttcatagaga ctgattcacc atatatgtca ccagagccac atagaggaaa
    13441 gagaaacaat ccttctcaag tatcttttgt tgctgataag atagcccagg aaaaagggat
    13501 atcttatgaa gaagtatgta gagttacaaa agaaaatgca aagaaatttt ttaatataaa
    13561 atagacaaat taaatcttat aatgatatat ataataagtc attataagta taggatgaga
    13621 gtgcctcaaa atgagttttt tagtttatga ggcactcttt ttgtatgaaa tcaaatatat
    13681 tttcatcatt tcaacaatga aaaagaagtt tatcatattt tgagatagtt tgtttttatt
    13741 tatttaattc atattttaaa atatattttc ttaacattgt taaaaaagaa tttacaagat
    13801 attcattaga attaactcaa ctctaatatt cacttaactt aagttgttta ttatattatt
    13861 aaagagaagt aaacgaaaag tagattaagg aggttgaatt ttgtatacag ttgatagtac
    13921 taagatattg ataaatatga acttagagaa actagaaaag atatactcaa tgataaaaga
    13981 tacatcaagt atttctataa ttaaagaacc taacttggct acagtaatgg taagagctaa
    14041 tgaaagtgta aaaaacacaa catttaattt aggagagata ttagttactg aatgtagtgt
    14101 aaaggtagat gaaagcttgg gttacggaat agtttcagaa aacaataata agaaagccat
    14161 ttatttagca gttatagatg cagttttaca tagcaataac agtaaatttg atgagttaaa
    14221 gaattacata aataaaagtg tatatgaaga gaacttaagg tatgaacaag agattattga
    14281 tgaatttagc ttgattaata aaacgaaagt tcaattcaat gctatggatt aaataggagg
    14341 agaatcatgg taaattatac acaagaaata tatagaaaga tgctagatag tgcttcattt
    14401 cctgggaaat taaataatat agataatata aatatagaaa ataatacaaa actattgaat
    14461 ggagcaattg gtatagcaat cactttatta gaccaagaag ttaccttcta tatagaaaat
    14521 tatactcaaa aagatataaa aaatataaga gcacttacaa tgtcaaatca aatagattat
    14581 agaaaatcag attatatatt tttagatatt aattcagaag tggatatatt acagattaaa
    14641 ataggttctt ttgaatatcc tgatgaaagt gcaactatag tacatcaagt aaatgattta
    14701 tcaacgtact ctcaaactaa gtatataaag cttgatttga gtggtcctgg tataaaatct
    14761 aaaaatagta tatatataaa tggaattaga gaagaattta tagaaagatt atctagcata
    14821 aataaagatt atccaatagg aatagatttg attttagtag ataaaaaagg agaaattgca
    14881 tttataccaa ggtcatcaaa attaagttgg gaggtattat agaaatggct tatgtagcag
    14941 ttaaaggtgg agaagaagcc atattacagg ctaaaaatat acttgagttt tatagggtaa
    15001 aaggagatag cttgcctata gatgtaaagc agattgagga acaaatgtct tatgctgtag
    15061 acaaagtaat gtctgaagga agtttataca acaaggaact atcagcattg gctataaaac
    15121 aagctcaagg tgatatatta gaagcctctt tctatgtaag agctttaagg tcaacaatgc
    15181 ctagaattac atattcagaa cccataaata cagaagaaat gagaataaaa agaagaatat
    15241 cagctacttt taaagatata ccaggaggtc agtatcttgg ggcaactaga gactatgaaa
    15301 atagaatatt gaatgtagaa ttattagaag aagatgaaaa aaagaataga cattttagag
    15361 atagttttat aaatgaatta gaaacagtag atgaaatccc tgaatttaca aaaatatcta
    15421 atatatttag aaaagaaaat ttattacaag aagaagtttg tgaaaacaaa ttggaagaag
    15481 ttattgatga tattacaaga aatagtttaa gatttccagt ttctaggtca gcaaagttac
    15541 aggcattaac tagaggtgaa agtggagtag tgtcaggttt tgcttatgct attgtaagag
    15601 gttttggtga tgaacatcca tatataagtg aacttagaac tggtaatgta tttataaaag
    15661 taaaacatcc catctatgaa gatgaagaaa taataatagg ggatatgctg gttactgaat
    15721 gtgaaatagt ttcaaaagca tcacaaaata agactgaaaa ttctaaaact aattcggaag
    15781 ataaaaataa gatgtcttta ggatatggtc tttgtatggg gaataatgag aataaggcta
    15841 tatctatggc catactagac aaatctttgg agccaaatag tgaaaaacta gcacaagatg
    15901 aagaatttgt attgcttcat atagatggaa tagattcatt aggatttata tctcacttta
    15961 agcttcctca ctatgtagat tttaaagcgg atgtagaaag aataaaatag tttgaggagg
    16021 taagtttagg tgatatataa ctatggtttt ttagacgaaa acacaaaaaa agaaataaga
    16081 agaaagatat taaaagcaat atcaatacct ggttatcaag ttccctttgg ttcaagagag
    16141 ttgcctatag ctaagggatg gggtactgga ggtttacaat taacactatc attaattggt
    16201 aaaaatgatg tagtaaaagt aattgaccaa ggaagtgatg attctacaaa tgcttgtaat
    16261 ataaggagct ttattgcaag tgtaagtaat gtagaaacca caaaagatac attagaagct
    16321 acattgattc aaacgagaca tagaatacca gaagaaaaac ttaaatctaa tcaaatacta
    16381 atatttcaag taccaatacc agaaaccctt agaatagtag aacctagtga agtagaaaca
    16441 agaagaatgc attcagaaga agattatagt aggatgtggg tatatcttta tgaagatata
    16501 gttagattta atgatatatc tatagcagtt gaatatccat gtaaagtaaa tgatagatat
    16561 ttaatgaatc catcacctat acctagattt gatataaaaa aattaaatat gtcagacaat
    16621 ttattcttgt ttggtgcagg tagagaaaaa agaatttatg ctattcctcc atatacaaag
    16681 gtagaacctt tagagtttga ggattataag tttgaagagg aaaagtttga aggaaaatac
    16741 tgtagtttat gtaacagtac aaatacattt ttagatgaag tatatgacag tgacactaat
    16801 gaaaagtact atagttgctc agatacaagt tattgtgaaa aagttagatt aaaaaataac
    16861 agtatagatg tgacaatagg aggtacttgg aatgagtaaa ataaatgact ctaaaagatt
    16921 agaaaatata gacaagcaag tattaatagg aagaaatatc aatcttattt atggagatgg
    16981 gtgtgaaaag tgttttgaaa gcacaggata tgaaagcaat aatatatgta agtattgtgg
    17041 ttctatagtt gcatgtaata atgtaaattt agaactttat gaaggagaag tccttggaat
    17101 tgtaggtgaa tctggttcag gaaaaagcac tttattaaaa atactgtttt tccaagaaca
    17161 tgcaaatagt ggagaagtat atatatccca ttacaataaa gacacaaata tactagagct
    17221 ttcagaccaa aaaaagagat acataaagaa tcattttatg ggtattgtgt atcaaaatcc
    17281 ccaccttggt ttaaatttag actttagtag tggtggtaat atagcagaaa aattattgat
    17341 ggcaaattta tataatgttg aaaaaataag agatagaggc aaagaacttt taataaaaac
    17401 taatatacct gtagaaagaa tagaccataa accaaaatac tttagtgggg gaatgcaaca
    17461 acgagttcaa atagcaaaag ctctttcaaa taaccctcca atattgctat tagatgaggt
    17521 tacaacaggt ttagatgttt ctgtgcaagc ggaagtattg gatttaataa gagaaatcca
    17581 aagagaactt aagatttcta tgatagttgt atcccatgac tttgatgtta taaagatgct
    17641 tgctgatagg acaattgtaa tgaaaaatgg aagtgtaatt gagtctggtc ttacagacca
    17701 aataatggag gacccacaac acccatatac tcagcaactt ataaattcat tgctataaat
    17761 atcgtttgta aataagcaaa atgggggttt ttaataaaat gaaagaaact atacttgaag
    17821 taaataattt tagtaaagac tttgagttac atgcattgaa caagactata acggcatgta
    17881 gtaatataag ttttgaagtt tcaaaaggtg agtttttagg aataataggt aaatctggag
    17941 caggtaagtc taccatatta aaaagtatct ataaaacata tataccaaca actggagaaa
    18001 taatttttaa ttcagaaata tatggtaatg tagacctggc taaaattgga gaccgagaaa
    18061 tgataaatct tagaaagaaa gaaataggat atgtatcaca gtttttaaag acacttccac
    18121 gaataactgc aattgaactg gtagtacatt ctttaattga gagtgggttt gaaaaagatt
    18181 gttcgtatga tatggcaaaa gatatactta accaatttga aataaaggaa aatttatggg
    18241 atgcgtatcc aaataatttt tctggtggag aaaaattaag attaaattta gctcaagcta
    18301 tggttaaaaa gcctagactt ttgttacttg atgaaccaac tgcatcactt gataatcaat
    18361 ctaaaatata tgtaaaagaa aaaatgttgg agcttaaaag tcaaggaact acaatgatag
    18421 gaatttttca tgatatagag tttatggaaa ctgttataga taagaccttt actatgacaa
    18481 aagggactat aagtgaaagt ggggtagcct agatgataaa actaattcat aatgcaaata
    18541 ttgtattaga agatatgatt ttagaaaatg gtcatttaat catagaggga gatacaataa
    18601 aaaaagtatc aaatgataag attgattacg catttgactt taatgagata gatgaaataa
    18661 tagatgcaaa aaacttatat gtaatacctg gtattataga tattcacagt gatgccattg
    18721 aaaaggaaat agagccgaga ccaagtacat tattaccatt taatatggca ttttatgaac
    18781 tagataaaaa actacctgca aatggaataa ctacagttta tcattctata tctttagggg
    18841 atggagtagg agttagaagt attgataact ctttaaagat gattaaaaat attgattcat
    18901 ataaaaatat agatagtaag agcataaatc ataaagttca tttaagatat gaagtgttgt
    18961 attatgaagg tttagaaaag gttttagaac tattagatga aaataaaata gactacttat
    19021 caataatgga ccattctcca ggacaaggtc agtatacaaa tccaacattt tataaagagt
    19081 atgctactaa agtgtgggga gttacagaaa attatgtaga tacatggtta gatgatttag
    19141 taaatctgca tgataattta gattggaata aaatagctaa tattataggt atagccaaaa
    19201 ctaaaaatat aaatgttgca tcacatgatg atgatacatt agaaaaaatg gatttcataa
    19261 aaaatatagg cataaatgta tctgaatttc ctataactct tgaagttgca aaacattcaa
    19321 agaaattggg tgttctaact tgtttaggtg caccgaatat tgtaagaggt aaatctcata
    19381 ataacaattt aaaagcaatg gatgctatat tggaagattg ttgtgacatt gtgtgttcgg
    19441 attatcttcc atcagctatg ataaagtcta tgtgtatagt tgcagaaaga ataaataatt
    19501 taaataaagc agtcagttta tttacctcaa atcctgctaa agcagttggt atatatgatg
    19561 aaagaggtag tataaaagaa aataaaaaag cagatttagt tttagtagat attgatagtg
    19621 aatatccaaa ggttgtaaat actattgtaa atggaaaaac agtttataaa agggaggtat
    19681 aaagattgct aacagagtta cattgtcata caagtatatc agataataat tttactactg
    19741 aagaaataat aaaaaaagca gcagaaaaga atataaatat cttagcaata acaaatcatg
    19801 acacattatc tggattggat gaagctcttg tgcttggaga gcattatgga ataacaataa
    19861 taccaggtat agaaatttca gcctatgatt ataaaaatag aaaaagagtt catattcttg
    19921 gatataacat agatttaaac agtcaagaaa taaaaaattt gtgcaatcca atggtatgtg
    19981 acagacataa agcatctgtt aagatggtaa ataaaattat agatttagga tacaaaatat
    20041 ctctggaaga tgtaaagaaa tattcatcaa agactggaat atttaaacaa catataatga
    20101 aagctcttat agataaagga tatacagata atatatattc aaatctatat aaagaattgt
    20161 ttcatagagg aaacggcaag gtatatgtat ctttaaaata tgtggattac agagatgcaa
    20221 taaaagctat aaaaaattct ggtggaattt gtgtgttagc tcacccgggt caaatggata
    20281 attttagcgc tattgaagag atggtaaaag taggattaga tggaatagaa gtatatcatc
    20341 catctcacaa caaagaacta gaaaaagagt cgctttctta tgctaagaag tataatctag
    20401 tggtcacagg aggttcagat taccacggat tttattctca acataattat gaacttggaa
    20461 gcaagagctt aaataaagaa gatttattta agtttaggtt agctttagga ggggtattat
    20521 aaatgtcttt tttacaagta aaaaatgtag gtaaaagtta tggacaagtt aaggtgttaa
    20581 aagacatatc tattgatatt gaaaaaggag agtttatttg tttactaggt ccaagtggtt
    20641 gtggaaagag tacactactt agaataattg cagggcttga ggataaacat ggtggaaaaa
    20701 taataataaa tgataaggat atgactaatt caccacctga aagtagaaat tttggtatag
    20761 tttttcaatc atatgcatta tttccaaata tgaatgttta caaaaacata gcctttggac
    20821 tagaaaataa aaacataagt aaatctaata ttgataaaaa agttaaagaa gttctagaag
    20881 ttgtagaatt aagtggatat gagaaaaaat atccatcaca attatcaggt ggacaacaac
    20941 aaagggttgc attagctcgt gcgattgctt tagagccaga ctttttactt cttgatgaac
    21001 ctttatctgc attagatgca aaagtaagac ttaaacttag ggagcagatt agaagtttac
    21061 atagaaaact aggcattaca actataatgg taacgcatga ccaagaagaa gctctttgtt
    21121 tagctgataa gatggttgtt atgaatagag gagaaataat acaagttgga acgccaaagg
    21181 aggtatataa aaatccagaa acaccttttg tagctgattt tataggaaca ataaatttta
    21241 tagatgatgg tattaataag atagctatta gaccagaaga tataaaagta gagtctaata
    21301 gagattctaa agataaggat ataaaagtgg gagaaatttt agatatcgaa tttagaggat
    21361 ttaactatag aattacagtt gagtatcgtt ctaagcaaat gaaattagat gtagtttcca
    21421 aagttgcaga acaaatgaaa ctgtgcatag gttctaaaat aaactttaag atacctaaag
    21481 aaggaattgt acaatataaa tcagagggat gtgcttaata gctaggggtg aataaatgat
    21541 aacatcaaaa aataaaagaa gaataagtga tagcacaata caaaaatatt tcttgatttt
    21601 ttcggtaatt gtactaatag tatttatatt atttccatta gtatcattat taaaaaatgc
    21661 atttatggat acaagtggaa attttatagg aatttcaaac tttagtaaat atgttgaaaa
    21721 tccaagtctt atagcatcat ttaaaaattc aatttttgta tctacagtct catctttaat
    21781 atcactatgt ttagcattta tatattcata cgcaataaca agaacaaaca taaagtttaa
    21841 agattttttt aaaactatgg gaatgtttcc actttttgca ccaactatgt tatatggtat
    21901 ttcacttata tacctttttg gaaataaggg aatctttaca accatgggtc tggaagtacc
    21961 actatatggt ccattaggaa taattatttc tcagattcta tttacatttc cacaagcttt
    22021 tttaatatta tctgtagcac tttcgatggc tgattataga ctttatgaag cagcagatag
    22081 cttgggtgct agtgaattta agaaattttg caaaataaca attcctggta taaagtactc
    22141 attacttagt tcattctttg tatcctttat acttgcattt acagattttg gagcagctaa
    22201 ggtagtcgga ggaaactaca atgtactggc tactgacata tataaacaag tagttggaca
    22261 atttaacata ccaatgggag ctactgtttc tatggtaatg ttaattcctg tactaatagc
    22321 atttacatta gataagatgg caagtaagaa acaaggtatg accataactg caaagtctat
    22381 gccatataag attaaggaaa ataagtttag agatagagta tttttaatag tatgtagttt
    22441 tataacattg ctaattttta ttttaatatt cgtttcagtt ataggttcag ttataaagtt
    22501 atggccatat aatttaagct ttacaatgga acactataca tttaaaggta ttttgggaag
    22561 tggaataaaa acatacttaa gcagtttgaa aatatcaact ttaacagcaa tatttggaac
    22621 tatatttgta ttttttagtg cttatatgat agaaaaggtg gataagtttc ctaaattaag
    22681 acaaacagct taccttttat caatgatacc aatggcctta cctggcttag ttttaggtat
    22741 atcatatata acttttttta actcagcaag taatccatta aattttttat atggaagtac
    22801 agcaatatta gttatagtaa atgtagttca tttttattca gtttgtttta taacctcaaa
    22861 ttcatcttta aaaatgttgg ataaagagta tgaattagtt gcaaaatcca taaatattcc
    22921 attctataaa gtatttttta atataacagt tccaatgtct ataacatcaa tacttgaaat
    22981 agtagtatat tattttgtaa attcaatggt tactgtttca gcattaatat ttttatatac
    23041 acctcaaact cagactgcat caatatcaat actaaagtta gatgagatag gatatatagg
    23101 aacatcagct gctatggcag tattagtgct tttaacaaat ataatagtaa gacttttgta
    23161 cgaatttgta acaaagaaat taaaaaataa cacacaaaaa tggcagcaaa aagatgttgc
    23221 ataggggagg taaaaatgaa atttaagaag ctagtttctt taggagtatt atcaacactt
    23281 ttattaagtg taataacagg atgtagtact gattcatcag gttcagattc aaagtctgat
    23341 agtaagacaa aaggggattt aacagtttac acagcaattg aagaagatag tatagagcca
    23401 tatttagcta catttaaaga gaaatatcca gatattaaat taaatattgt tagggcatca
    23461 acaggagata taacagcaag attattagca gaaaaggata atcctcaagc agatgttgta
    23521 tggggagttg cagccacaag cttattggtt gcagatgacc aggggatgtt agaaccatat
    23581 gctccaaaag gttctgaaga aatagagtct gcatttaaag atgataaaga agttccttct
    23641 tgggttggga tagatgcttg gatgacaggt ataacagtaa acactaaaga attaagtgat
    23701 aagaacatcc cagttcctag ttcttacgaa gatttaataa agccagaata taaaggtctt
    23761 atatctatgc ctaatccatc ttcttctggt acagggtact taacagtatc agcccttata
    23821 caaattatgg gtgaagaaaa agcatggcag tatatggata aattgcatga gaatattggt
    23881 gtatacactc aatctggttc agctccagct acatcagcag cttcaggaga atatcctatt
    23941 ggtatatctt ttggatatag aggtataaag ttaaaagagg aaggctatcc agtagaagta
    24001 gtatttccaa aagaaggttc tggatgggat atagaagcaa atgctcttgt gaaaaaagat
    24061 aatataaaag aagcatcaaa agtattttta gattgggcta taagtaaaga tgtaatgaac
    24121 gaatactcta agaactatgc agttactact atttctacag gaaatccaat accagaagga
    24181 ttccctaaaa agccattaga gcaaatgata gataatgatt taaaatcagc agcaaaaaat
    24241 agagaagata tactaaataa atggatatca aaatatgatg gtaagacaga aaaagagagt
    24301 taagtaaaac tatacaaatg gagtaaaaag aaagaatttt tttaagtaga ttttattata
    24361 ataatataag ttgagaataa tataggtcaa gaaaagtgaa tattctataa aaatgtatag
    24421 agtaagtata ccattcagtt tgaatggtat attttttatt tttagagatt aatttagaaa
    24481 tagatatact acagtttaaa atagtatatt ttaatttcat tattaaagta tagatattat
    24541 atataaagta aataaattat tgagcgatta ttaataagat atgtataaat tttaatttgg
    24601 gaacttgtgt tatatatcat gaaaaaagag aaaaaataaa aggtaaattg tttcttgata
    24661 acttgaaatt ggaagtgaga atatttaact agagaagaat tatttaagtt taagtcagct
    24721 ttaggaggag gaattataaa tgtctttttt acaagtaaaa aatgtaggta aaagttatgg
    24781 acaagttaag gtgctaaaag acatatctat tgatattgaa aaaggggagt ttatttgctt
    24841 attaggtcca agtggttgtg gtaaaagtac attgcttaga ataattgcag gacttgagga
    24901 taaacatgat ggaaaaataa taataaatga taaggatatg actaactcac cacctgaaag
    24961 tagaaacttt ggtattgttt tccaatcata tgcactattt ccaaatatga atgtatataa
    25021 aaatatagcc tttggattag aaaataaaaa tataagtaaa tctgatatag ataagaaggt
    25081 tagagatatt ttagaagttg tagaattaag tggatatgat aaaaaatatc catcacaatt
    25141 atcaggtgga caacaacaaa gggttgcact agctcgtgcg attgctttag agccagactt
    25201 tttgcttctt gatgaacctt tatctgcatt agatgcaaaa gtaagactta aacttaggga
    25261 acagattaaa agtttacata gaaaactagg catcacaact ataatggtaa cacatgacca
    25321 agaggaagct ctttgtttag ctgataaaat ggttgttatg aataatggag aggtaataca
    25381 gattggaact cctaaagaag tctataaaag tccaaaaaca ccttttgtag ctgattttat
    25441 aggaacaata aattttatag atgatgatgt gaatatgata gcaattagac cagaagatat
    25501 aaaggtagaa tttaatagag actttaaaga aattgatgta aaagtaggag agattttgga
    25561 tatagaattt agaggattca actatagaat tacagttgaa tattgttcta agcaaataaa
    25621 gttggacgta gtttcaaagg tagtggaaca aatgaaacta tgtgtaggtt ctaaaataaa
    25681 cttcaagata cctaaagaaa gaaccataca atataaatca gagggatgtg cttaatagct
    25741 agaggtggat aaatgataac aataaaaaat aaaagaagaa taagtgatag taagatacaa
    25801 aaatgttttt tagttttttt agtgattgca ttagtagtat ttatattatt tccattaata
    25861 tcactattaa aaaatgcatt tatggataca agtggaagtt ttgtaggaat ttcaaacttt
    25921 agtaaatata ttgaaaatcc cactcttata gcatcattta aaaactcaat ttttgtatct
    25981 acaatttcat ccttaatatc attatgttta gcgtttatat attcatatgc aataacaaga
    26041 acgaatataa ggtttaaaaa cttttttaaa actatgggca tatttccgct tttcgcacca
    26101 gctatgctat atggtatttc acttatatac ctttttggaa acaagggaat ctttacaatt
    26161 ataggcatag aagtaccact atatggtcca ttaggaataa ttatttgtca aatactgttt
    26221 acatttccac aggccttttt aatattatct gtagcacttt cgatggctga ttatagactt
    26281 tatgaagcag cagatagctt aggggctagt gaatttaaga agttcttcaa aataacaatt
    26341 cctggtataa agtattcatt agttagttcg ttctttgtat cttttatact tgcatttaca
    26401 gattttggag cagcaaagat agttggagga aactacaatg tattggctac tgacatatat
    26461 aaacaagtgg taggtcaatt taacatacca atgggagcta ctgtctctat ggtaatgtta
    26521 attcctgtac taatagcatt tacagtagat aagatagcaa gtaaaaagca agggatgact
    26581 ataactgcaa agtctatgcc atataagatt aaggaaaata agtttagaga tagagtattt
    26641 ttaatagcat gtagttttat aactttatta atctttattt tgatatttgt ttcagttata
    26701 ggctcagtta taaaattatg gccatatgat ttaagcttta caatggaaca ctatacgttt
    26761 aaaggtatat caggaaatgg aatcaaaaca tatttaagca gtttaaaaat atcaatttta
    26821 acagcaatat ttggaactat atttgtattt tttagtgctt atatgataga aaaagtggat
    26881 aagtttccta gactgagaca aatatcttat tttttatcaa tgataccaat ggcattacct
    26941 ggtttggctt taggtatatc gtatataact tttttcaatt cagcaagtaa tccattaaat
    27001 tttttatatg gaagtacaac aataatggtt atagccaata tagttcattt ttattcagtt
    27061 tgctttataa cttcaaattc atctttaaaa atgttggata gagagtatga attagttgca
    27121 aaatctataa atattccatt ttataaggtg tttttttatg taacacttcc aatgtccata
    27181 acatcaatac ttgaagtggc tgtatattac ttcgtaaatt caatggttac tgtttcagca
    27241 ttaatatttt tatatatacc tcaaactcaa actgcatcaa tatcgatact aaagttagaa
    27301 gagatagggt atatagggcc atcagctgct atggcagtat tagtgttttt aacaaatata
    27361 atagtaaaac ttttgtatga attgacaaca aagaaattaa aaactaagac acaaaaatgg
    27421 caacaaaaag aagttgcata gaggaggtaa aaatgaaatt taggaaatta gtctctttag
    27481 gaatattatc aacactttta ttgagtgtaa taactggatg taataatgct tcatcaggtt
    27541 caaatttaaa gtctaataat aagataaaag gagagttaac agtttataca gcaattgaag
    27601 aagatagtat agatacatat ttaactacat ttaaagagaa atatccagat attaaattga
    27661 atgtacttag attatcagta ggagatataa cagcaagatt attagcagaa aaagataatc
    27721 ctaaagcaga tgtgttatgg ggggttgcag ctacaagttt gataatttta gatgacaaag
    27781 aaatgctaga gccatacgct ccgaaaggct ttgataaggt aaatcctgta tttaaagatg
    27841 ataagaaagt tccttcttgg gttggaatag atgcttggat gactggtata acagtaaata
    27901 ctaaagaatt aagtgataag aacataccag ttcctagttc ttatgaagat ttaataaagc
    27961 cagaatataa aggtcttata tctatgccta atccagcttc ttctggaaca ggatatttaa
    28021 cagtatcaga acttatacaa attatgggtg aagaaaaagc atggcagtat atggataaat
    28081 tgcataaaaa tattggtgta tacactcagt ctggttcagc gccagctata ttagcagctt
    28141 caggagaata tcccattggt atatcttttg gaaatagagg tataaagtta aaaggtgaag
    28201 gttatccagt agaagtagta tttccaaaag aaggttctgg atgggagata gaatcaaatg
    28261 cacttgtgaa aaaagataat ataaaagaag catcaaaagt atttttagat tgggctataa
    28321 gtaaagatgc aatgaatgag tattctaaga attttgcagt tactacaatt gacacgggaa
    28381 atccggtgcc agaaggattt ccaaagaatc cattagaaca gatgatagat aatgatttaa
    28441 aatcagcagc aagaaataga gaggctatac taaataaatg gatagaaaaa tatgatggaa
    28501 agacagaaaa agagagctaa gccttgcaat aaaatttagt acaaaaagcg cctagaatat
    28561 aagttttata tctaggctct tttgatatat ttatttaaaa tgcatatttc tatataaaaa
    28621 taaatagttt agtaccttga ttaaattaaa taaaaacctt aaaataaaga gatgtaaaga
    28681 aaaactaaaa acgaataaaa ggtgaattta tatgataaaa gaaataatag tagtagaggg
    28741 tagagatgac gttacagcag ttaagagagc aatagatgca gaacttataa ctacaggtgg
    28801 atttggattt ccaaaaggtg taatggagcg tataaaagct gctaatgaaa gacgtggtgt
    28861 tataatattt acagacccag attttgcagg tgaaaaaata agaaaaaaaa tagcatcaga
    28921 agttccagga tgtaaacatg catttctacc gagagaggaa gctaaaaagg atggagatat
    28981 aggtattgag aatgctactc ctcaaagtat aatagcagct ctgaataaag ttagaactga
    29041 aaatacagaa aaaagagatg aatttaagca agttgactta attagaaatg gtcttatagg
    29101 taatgaagaa gcatcactta gaagagatgc acttggaaaa atacttggaa ttggatatgg
    29161 aaatgcaaaa caatttttaa atagattgaa taattatgga gttgaaagag aagaatttat
    29221 agatgcatta aaacaaatct aaacgtaatg tttaatgtat gaaaaatatg tatataaaat
    29281 aaattgtgga attacggtta atttaagatg aattatttat aatatacacc ttattttaaa
    29341 tcaatgtaaa tacatataaa taagtataat taataaaatt aaatatcaaa ataagttaaa
    29401 aaggagtaaa taatggatag actatcttca cataatgcaa caaaggaagt agtacaaaaa
    29461 tataatttta aattttcaaa gtctttaggt caaaatttct tgatagatag taatataatt
    29521 gataaaatct tatctggagc tagaatcaca agaggagata atatcataga agtaggacct
    29581 ggtataggaa ctcttactcg tgagatgggt aaaatagcag aaaaagtagt ggctatagaa
    29641 atcgatagaa atttaattcc tatattgaaa gataccttgt cagatttaga taatacagag
    29701 gttgtaaatc aagatatact aaaagtagac atacaggaat tagtaaaaga taaattaaat
    29761 ggtgggccag ttaagcttgt agcaaatctt ccatattata taactactcc aatagttatg
    29821 aaatttttag aggaagacat acctgtaaca gacatagtag tcatggttca aaaagaagtt
    29881 gcagatagaa tgaatgctat tccgggtact aaagattatg gggcattatc tatagctgtt
    29941 caatactatt gtgatacaga gattgtggca aaagctccaa gacatatgtt tataccacaa
    30001 ccaaatgtag attccacagt tataggtcta catgtaagag ataaaagaaa atatgatgtt
    30061 cataatgaag acatattttt taaaacagta aaagcatcct ttggacaaag aagaaaaact
    30121 cttcttaatt cgttgggagg tcttggtttt cttaataaag atgaaataag agaagtatta
    30181 aaagaagcta atatagacga aaaaagaagg ggtgaaactc ttagtataga agagttttca
    30241 gttctttcaa atataataaa tacaaaagta tcttctaaat agaaggtact ttttttaatt
    30301 ttgttaacat atattgttta gagaaactgt gttaaggggg gattgcagtg ggttctaaaa
    30361 gaaagtttaa aagagactat ataatactag aagctaaaga cataaacttt agatacaaag
    30421 aacgagtact gccaaaagct tttgcaaaag ttgaagtaaa tgatgaaaaa gcaattattg
    30481 cattatatgt agaaaatcta aaatatgtta aagatgggta taaagcagta gcaataaaag
    30541 atgaccatgg attaatagac ttaggaaata taattttaaa tgaacaagga agaggtgaat
    30601 ttgttttaga tttagaagat tatgatgatg agataaaagg tattgcatta ttgtatgaaa
    30661 gaaatatacc tttaattgga tttaaaggaa ataagcttga aaattatgag gacatattat
    30721 ttcaagaggt agaggaagaa gaatatgaag aatatgaaga gttggaagaa gaagacgaag
    30781 agatagagga taatgaggaa agtgatagag acgaaggtag tgaagaagaa tatattatag
    30841 aagaatatga tgaagatgag tatgaagact attgggaaga tgatgaagat attgacgaag
    30901 gagagttaaa agcaagaacg gaagtagaag attctggtga tgaggaaata gaagagttca
    30961 ataattataa ggaaatagaa aattataata gggttaaagt acaggaagaa tatatagaca
    31021 tagaatatga tgaggaagaa gaggaagaag aagaagtttt tgaagtaaat agagaagaag
    31081 ataggtttgt ttcctcaaaa cctactaaat caaagaaagt tcaaaaagat cagaataata
    31141 ttgaaaaaat aaaaagagct aatggagtaa gtcaaggaga gagtggaaat aaacaaacca
    31201 gtacattact tatgccaaga caaataaaaa aaggtttgaa attatttaaa gaagtaaaac
    31261 cttttgtaac agattatata gataagacaa gatggtggaa aatagagata aatcctacaa
    31321 cattgtgtgg atatacaatg ccttatttag gatatttaaa cgtgttgaat tacaccatgt
    31381 atagtgatgc agttttacat tcatataaat acagacacta tttatttgga gtccagtatg
    31441 atgaatataa taagagaaaa cattatattt atgctatccc aggaaataga aatgaacagc
    31501 cagacaatgg aagcacagga tttttaagat atcaagcttg tgataataga acaaatagtt
    31561 taggatattg gctatgtttt atagattcta gacaaagaaa tatagttagg taaaaataat
    31621 aaaaatgaaa aaatttagac tcaattctta ttttactgaa aaaattaatg aaatgtagta
    31681 caaaacttta aaaactattg ataatattta aatattacac tacaatatat cttgaaatac
    31741 ctattatatt atggaggaaa attatgataa acgagcaaag aattttaaat gagtttcttg
    31801 aattagtaca gatagatagt ttatctttaa aagaggggaa tgttgctaaa gtattagtta
    31861 aaaaattaga agaaataggt tgttcagtag taatagacaa tgctggtgaa aaagcaaatg
    31921 gagaaactgg taatataata gctacattaa aaggaaataa agaaggaaag aaaatattat
    31981 ttagttcaca tatggataca gtaactccag gtataggggt aaaacctata gtggatgaag
    32041 caaatggaat aataaaaagt gatggtacta caatattagg ttctgatgat aaagcaggta
    32101 tagcagctat attagaaggc ttaagatata taaatgaaaa taatatagaa catactgata
    32161 tacaagttgt attctctata tgtgaggaat gtggattagt tggagctaaa aatttagact
    32221 atggaaaaat agattctgaa tatgcattta tattagatag tggtggttct ccaggtgaaa
    32281 taatagtaaa agctcctgca caagatgtaa taaatgttaa gatattagga aagactgctc
    32341 atgcaggtct tgaacctgaa gctggaataa gtgctataat ggtagctgcc agagcaatag
    32401 aaaatatgaa tttacttaga atagacgaag aaacaactgc taacatagga attataaacg
    32461 gtggaacagc tacaaatata gtaacaggag aagttaatat aattgcagaa gccagaagtc
    32521 taaaagaaaa taaattagat gttcaaacta aacacatggt agagactttt gaaaaagctg
    32581 caaaagattt tggagctcaa attgagatag atgtcaatag agcatataca ccaatagatg
    32641 ttagtgagga ttctgaaata ataaaacttg caaagaaggc tttttcaaac ttaggtatag
    32701 aaggtcatac tgaatctaca ggaggaggta gtgatacaaa tatattaagt aagaatggta
    32761 ttgaagctat aactttagga attggtatga aaaatgctca tacactaagt gaacatatag
    32821 ctataaaaga cttatatgat tctgctaaaa tggtagtgga gataataaag gaagcataaa
    32881 tttaaataat agtgtataat atatatatcg cctagcttaa gctaggtgat ttttcatttt
    32941 atagaagatt gataatctct attgaaagta aagagtatac atttgcatat aagagagtgt
    33001 ttaatttatg ttcaaatgta ggttcttaat aatatttaga ttgtttcata agggaggaat
    33061 aaatatggaa acaaggtatg aagaagcaaa taaaataaca atacaatcaa tactttggaa
    33121 tgtagtatta acaataatta aggttatagc tggtgttatt ggtaattcta gcgctatgat
    33181 tgcggatgga ttacattcag catcagatat tataagttct ataggagtac taataggcaa
    33241 ttatgtatct tctagacctg gagatagaga gcataattat ggtcatgaga aagcagagac
    33301 attagtatca tttgtgttat caatattgct tattttcgtt tctataacaa taggtataga
    33361 ggcaattaaa tctttattta acttagatgc attaagtgtt ccatcaatat taccattagt
    33421 agtatcagtt atttcaatcc tgataaaaga gtatcaatat agaataacaa taaaagttgc
    33481 taaaaagata aattcaccag ccctaaaagc tgatgcttgg catcataggt cagatgcttt
    33541 atcttcagta gctgctttta taggtatagg tggttctatt ttagggttta aaccattaga
    33601 cccaattgcg tcagttgtag tggctatatt tgtagctaaa gtaggaatta gtatacttat
    33661 aagttctgtt aatgaattga tggatgtttc agttgatgaa gaagagataa aagaattaaa
    33721 atttattgta gcagatacag aaggtgttaa aaatcttggt gatataaaga ctagaaaaca
    33781 tggagctatg gcatatgtag atttgactat atgtgtggat gaaaatttga cagttaagca
    33841 aggtcatgat attgctacca agcttgaaaa acatataata aaacatatgg aatttgtaaa
    33901 aggcataaca gttcacgttg aaccatgtac taattgccaa ggaaataagt gtaataatta
    33961 attaaaactt aaaaaaaagt gtaaaaagtt atataattgt tttatgatta taaactctag
    34021 gggtggttaa atgagcaaga aaagaaaact aaagaaaaaa gctgcaatat taataggagt
    34081 agttgttttc tttataatat actttatatc ctttagaata ggaataaata tggaaaagga
    34141 taaaagtgaa gtgccaaaga aggtagaaaa agaagaagaa gataataggt ctttaaggac
    34201 tcaaataaaa gagaagaaga aggtttatat atcagataaa aatatagaaa acataaaaat
    34261 tgaagaagat atgtgggatg aaataaagtt atttttctca gattttaaga aagtaagaaa
    34321 tactacttct tcttacgaag cagtttatga aggttattct gatgatggag ttaaattttc
    34381 tactgacctt aactttttta gagtatatac tgtaaataaa gaagaatatt ataaagtacc
    34441 agttagttct aaggatgctt ttaagaaact tttagatgag agcatatata cttcgtttga
    34501 ccttttaagt cattataaag attggaaaga ggttactata actcatggaa aagaaattaa
    34561 aaaagttcat agatggaaat ttgatgattt agcacataag atgatttcaa agagggttgt
    34621 aggaaaagtt caacctgaga aaagtaagga aagaagtgac tataatttta ctataaatat
    34681 aaaaggtgat aattttgatg taaaagttga tacaatggga aaagattatg taaaagttac
    34741 ttctaagaaa aatgtatctt actatgaggt tcatacaagt ttatttgatt accttaaaaa
    34801 tgagatattt gaaattggaa agaaataaga ctttaagaag gctatcaaat tagatatttg
    34861 atagcctaaa ttattttata tattattttt caaccaacaa gtctgcaacc ttataaattg
    34921 gaccagcacc agcagttatt actaaatcat tatccttaac attttcacgt aagtatttta
    34981 ctatatcttc aaattctttt atatagatgg catctacatt gttttgatat aatttctcaa
    35041 ccaagtcctt agaatgtata tctcctgggt ctttttccct agcagcatat atatctgtta
    35101 tgataacttt atcagcagca tagaatgcct ctgaaaattc acctaaaaga gattttgttc
    35161 tagtataagt atgtggctgg aagatacacc ataaagttga tttttttaat tttttggcag
    35221 cagctaaagt tgcttttaac tcagttggat gatgtgcata atcatctact actaaagcat
    35281 tcttataata ccctttagtt tcaaatcttc ttccgacacc tttataaatt tttatatttt
    35341 ttcttatagt ctctaagtca ataccagaaa caagagctgc cattatggca gaagaagcat
    35401 tgtatatgtt atgaagacca tatacagaaa gttcaaattc tcctaaatct cttccttcat
    35461 attctattct aaatattcca tgaccactat tgtcaaagtg aatatctttt attacagcat
    35521 cattgtcaga atctctgccg tattttataa tagtagcttt aacatcatac aatatgtcat
    35581 caatattttc atcatcgcca tttattatga aatagccttc tgggggtaat aattttccaa
    35641 atttgttaaa agaagctttg atttcatcta ttccagagaa ataatcaaga tggtcttctt
    35701 caacatttaa aactatagat atctttgggt taaaattaag gaaactatct acatattcac
    35761 atgcttcagt tataaagtgg ttggaattac ctatcttaac attaccacct atcatactta
    35821 agttaccacc aactagtata gttgggtcta agtctgcata ttcaaagatt gtagataaca
    35881 tagatgttgt agaagttttt ccatgtgttc cagaaactgc tatagaattt ttatattccc
    35941 tcatgatttg gcctaaaaaa gtcgctctgt tcattaccaa tttatttttt tctttagcag
    36001 ccacaagttc ttcattgtct ggatgaactg ctgctgtata aacaaccata tttacatcat
    36061 ctgatatatt atcttttttc tgaccaatat aaatagttgc cccttggtcc ctaagtttgt
    36121 ctaataggta tgattcgttt gaatccgaac ctgaaacttg ataaccttta ttgatacata
    36181 tttcagctaa tgcactcatg ctgataccgc caattcctat aaagtgtata ttcataataa
    36241 aaccaccttt ctttttaatt aagtcctaca atatgaacta tttctgtggt ataataatat
    36301 aatgttcaat attttataat taatatgcat ataatattat atgtatttta tttttgaata
    36361 taataaaaca taagtaatat tataccacaa taagaattgt tatgcattag ttagtttaac
    36421 gttatataaa ggtacttgta ggaggaaata ataatgaaat ttaaaagaac agagagaatt
    36481 ggagcaatag ttaaaatact gtctgataat ccaaataaaa tatatacttt aagttatttt
    36541 acaaatcaat ttaatgcagc aaaatcaaca ataagtgaag atttattagt tgtaaaaaat
    36601 gtatttgaga agctacattt aggtaaagtt ataactattt caggagcagc aggaggagta
    36661 aaatatatac ctaaaacttc tatagcagaa aatgcagagt tcttaatgga attatgcgag
    36721 agaatatgtg acaagtctag aatactatct ggtggttttt tatacttgat tgacctaatt
    36781 tacgacccta caatagctgc taagatagga aagatattcg catcaaatat tgagtatgta
    36841 gatgcagatt atgttgtgac aatggaaaca aaaggtatac ctatggcact tatgactgca
    36901 aaagctatga atttacctct agttataata agaaaagata taaaggtatc agaaggtcct
    36961 acacttagca tgacatatgt gagtggaaac agttctaaag ttgaaagtat gagtttgcca
    37021 agaaaagctc ttaaaccaga tagtaaagtt ataattatag atgatttcat gaggggtgga
    37081 ggtactataa aaggaatggt agaccttatg aatgaatttg gtgcagaggt tataggtact
    37141 ggagtattca tatctacaac gaatccttca gaaaaaatgg taaaagatta tatttcgttg
    37201 attcaattgg atgttaatgg agataaaata gtagtagaac caaacttaaa gacttttaaa
    37261 gatgaatata gaaatgaaga tttagacgaa gaagaagatt cagaatttga atttgagata
    37321 gatgaagatt aataaaaaaa atatttagaa aaatatgtaa aaataaaaag aggatatccc
    37381 tagttgttca tagaattatt taaaaaacag tttatattaa tttattccag cgataataat
    37441 tttaatataa attaatttct gtcaaaatca aagggggata ttttggtatg aagataactg
    37501 acgtaagagt aagaaaatta acagaagaag gtaagatgaa atgtatagtt tcaataactt
    37561 tcgataattt gtttgtagtg catgatataa aagttataga aggccataat gggctattta
    37621 tagcaatgcc tagcagaaaa gtaggcgaag gaaatttcag agatatagct catccaataa
    37681 atgcagaaat gagacaagtt ttagaagatg cagttttaca agcgtatcat gaagcattag
    37741 ttcaatggga agtagctgct gaataataat aaatatataa aatggagaga gcccgaacat
    37801 ttaggctctt tttaaatgta gaatttctat taaaatatgt actttttagt aaaaaagtgc
    37861 tatattatta gtgataatgt atatgttgta tacaaattgt tagataaatg tattataatg
    37921 tatattgatt aagagaataa tacgaataaa attaaattag attgaatgga gtgtatcaat
    37981 gaactttaaa gctataatcc ttgctgctgg caaaggaaca agaatgaagt ctaaataccc
    38041 aaaagttata cataaagtat gtggaaaaga aatggttaac catattatag atgtttcaaa
    38101 aaaatcaggg gtaaaagata ctgtcgtgat tttaggacat gaagctgatg tagttaaaga
    38161 aaagcttgca gaagaaatta taatagccat gcaaacagaa caacttggga caggacatgc
    38221 tgtaaagatg gctaaagaat atattaatga tgaagataca atagttgtac tttgtggaga
    38281 tactccactt ataaaagaag agacactaaa gagactattt gaatatcata tagaaaataa
    38341 atatcatgct acagttctta caacaagagt tggaaatcca acaggttatg gtagaataat
    38401 aagagataag aagggtgacc ttcttaaaat agtggaacaa aaagatgcaa attcagaaga
    38461 aaagatgata agtgagataa actctggaat ttattgtttt aatggaaaga gtcttagaga
    38521 agcactagat ttgctaaata ataacaattc acaaggtgaa tattatttaa ctgatactgc
    38581 taaaataatg agagataaag gattaaaagt tggtgcattt gcaggttcaa ctattgaaga
    38641 acttatgggc gttaactcaa gagtagaatt atcaaaggca gaagaaatta tgagacgaag
    38701 aataaatgaa tctcatatgg taaatggagt aactataata gacactaact ctacctatat
    38761 agaatctgat gttatgatag gcaatgatac tataatatat ccaggtgtta tgttacaagg
    38821 aaaaacaagg ataggttcag attgtataat tggaatgaat tcatctataa caaattcaga
    38881 aataggtgat ggtacagaaa taaaaaattc aactataatt gatagtaagg ttggagaaaa
    38941 ttctactgtt gggccatatg catacttaag accaaagagt gacttaggaa ataatgtaaa
    39001 aataggcgat tttgtagaag taaaaaatgc tattatagaa gatggctcaa aagcatctca
    39061 cctttcttat ataggagatg cacatgtcgg taaaaatgta aatattggat gtggagtagt
    39121 atttgtaaac tatgatggta aaaataaatt taaatcaata gtaaaagaca atgcgtttat
    39181 aggttcaaat tctaatttag ttgcaccagt agttgtagaa gaaaaaggat atatagcaac
    39241 aggttcaaca ataacacatg atgtcccaga tggtgctttg gcaatagcaa gagaaagaca
    39301 agttatcaaa gaaggatggg tagaaaaaaa gaatcaaaaa gacgaccaaa gtaagtaatt
    39361 atgtagttta ttattttggc taaaaaattt agctcgaaat tattataaat tatattcatt
    39421 agtattttaa aattttcagg agggttacat aaatgaatac tagcggaagc gagattaaaa
    39481 ttattgcagg taattcatcg aaagagttag cacaaaaaat agctgactat ataggtgtat
    39541 cagtactaga ttgtgaagta ggtacattta gtgatggtga aatatgtgta aacatgaatg
    39601 agacagtaag aggttgtgat gtgtttgtag tgcaatctac taacagccca gtaaatgata
    39661 acttaatgga gttattaatc ttaattgatg cgttaaagag agcatcagca ggtagaataa
    39721 cagcagttat tccttactat ggatatgcaa gacaagacag aaaggctaag gcaagagatc
    39781 caatcactgc taaattagtt gcaaatttaa taactgctgc tggagcagat agagtattaa
    39841 caatggattt acatgcagct caaatacaag gatattttga tataccatta gaccacttac
    39901 taggtggaac aatattagca aattatttta atgaaaagaa aatagaagat ttagtagtag
    39961 tatctcctga cttaggaagt gttactagat caagaaaatt tgctaatact ttaaatggag
    40021 aagttccaat agctataata gataaaagaa gaccgaaagc taatgtatgc gaagttatga
    40081 acttaatagg tgatgttaaa ggtaaaaatg ttatactatt agacgatatg atagatacag
    40141 caggaacaat agttaatgct gcaaatgcac ttaaagagtt tggagcaaaa gatgtttatg
    40201 cttgttgtac tcatggtgtg ttatcaggtc cagctattga aagaattgct aattcagaaa
    40261 taagtgagtt aatagttctt gatactatac aacttcctga agagaaaaga atagataaga
    40321 taaagataaa aacagtagct cctttatttg gagatgctat aagaatgata ttctctaatg
    40381 aatcagtaag taaattattc taatttattg tatttatgag agttgtttaa aaggatataa
    40441 accttttgag caactctttt ttgattttat aattatggaa tgttattttt ttcttgttag
    40501 aaaggtatat attattgtaa aaagagatta tagtttaaaa ttatgtatat ttaagtaaaa
    40561 taaacttaat aaagggcata ttattcaaaa tattatgaat ataaaaaata ttacaaaaaa
    40621 gtattgaaaa aattcagatt atgtagtatt atgtaaatat aaataaagaa agaaagaggt
    40681 gtttccaatg ggaaggttag aagatgaacc cctaataatt aaatgattca gtcacaatct
    40741 aaagtgaata taaaaatcta ttgaaagttt atttataaga attgaaaata gtatctattc
    40801 tatctttagt gtttgtgaga gggttctgga ttatataaga ttactgtaaa gaagtaattt
    40861 tgcacaataa gcatatctgt aaatgaagag tttcgtttat ataatgattg tggctgaatc
    40921 ataactataa tagatagaat gtttataata gatgatttta taaaatacta tattgtaata
    40981 gcttttagct ttcaaataaa tattagcaaa ggatggaaat gacaatgata aaactaaatg
    41041 ataatttata aatatctcct tacgatatat aataattgta gggagatgtt tctttttttt
    41101 gtagagtata atttttctag aaattctgtt tcaatatgta aaaagttggt atatataaat
    41161 agtataaaca gtgttgctaa aatatggtag atattatata atataaatgg atatgtatat
    41221 acattatgag ttatataatt tgaatttaat aaaatagaaa cgttgattta tgttctgtaa
    41281 tgtgggcacc ttggagcata ttgagttagt ggtgcaaccg gctatgaaat tgtatttatt
    41341 tataaatact attattttca tagctttttt tatttaaaaa atggttatat gttagaaata
    41401 aatttggtta gtacgtttaa ataattttac ttagattttt aaccagatat attataagta
    41461 agtattaatt aaaaaattta agggggaata aaaatgaagt taaaaaagaa taaaaaaggt
    41521 ttcactttag tggaattatt ggtagtaatt gcaattatag gtatattagc agtagtggca
    41581 gttccagctt tatttagtaa tataaacaag gctaaggtag caagtgttga gtctgattat
    41641 agctcagtta agagtgcagc attatcttat tattcagata ctaataagat accagttaca
    41701 acaactgata aagctggttt aactatttta gagacttata tggagtcttt gcctgataaa
    41761 gctgatatag gtggaaaata taaattgatt aaagttggta ataaattggt attacagata
    41821 ggtacagata ctgaaggagt tacattaaca gaagcacaat cagcaaaatt attgagtgat
    41881 ataggtaaag ataaaatata tacaggtgtt acaggagata attttggaga cgaattaaca
    41941 gatactacaa aaatagataa taaagctcta tatatagtac ttatagataa tactgtgatg
    42001 gactcaacaa aataattaaa actatatttt tataaagaat attgtaaatt taatgaaaaa
    42061 tataatataa aagacaggaa gaaaatcttc ctgttttttt atactatttt ctttaatgtt
    42121 caattatgaa tgatgatata attgagatag taaaggagtg aaacctgtgg ctaaaaaagt
    42181 tagaatagga gacaaactgg tcgaaaaagg atatataaca gaagaacagc ttaaatgggc
    42241 actatcagaa caaaaaaaca gtggaaaaag actaggagaa tttctagtac aagaagggct
    42301 tatagatagt aacttactaa taagtgtatt aaaagaactc ttggatatag aaagtatatt
    42361 tttagaaggg acagaaatag acactttagc tacaaaaatg gttccagaaa atatatgcaa
    42421 aagatatact gtatttccat ttaaaattga tggaaataaa atatgtctag ccatgtcaga
    42481 cccacaagat agagaagcag ttcaagatgt aaggcgtatg tctggtaaag acgtagagat
    42541 atttatatca agtacagaag atataaacaa agcaataggt catgcttatg cacattcaga
    42601 aataaataag gcaatgactg agtacaacaa aaatagaact ggtggagtaa gagaaacagt
    42661 aatacttgaa gaagatgtaa atgctgctcc aatagtacga ttagtaaata acatactaga
    42721 aaatgctgtt agaatggaag caagtgatat acatatagaa caaagtgaaa attacatgcg
    42781 tgttaggttt aggatagatg gaatgttaag agaatatatg aggatgaact cagcaccata
    42841 taaggcagta atcagccgta tcaagataat gtcagatata aatatatctg aaaagagaat
    42901 accacaggat ggtagaatct atttaaaagt ggataataaa cccatagatt ttagggtttc
    42961 tacaatgcca actaacaggg atgaaaagat agctatgagg gttcttgata agagtaattt
    43021 tatggttagt aaagaagtac taggaataga cgaacatggc tctaagatat atgatgaatt
    43081 gataaacact ccttatggtc ttatattggt tgttggccct actggaagtg ggaaaaccac
    43141 tacattgtat tcaatgttaa atcaactaaa tacagaaaat agaaatctat taacaataga
    43201 agaccctata gagtatgaat tacctggtgt aaatcaatct caaataaatg aaaaggcagg
    43261 gcttactttc gccagcggac ttagagcttt tatgcgtcaa gacccagata taataatggt
    43321 tggagagata agagatactg agacagcaga aatagcaatt agagcttctc ttacaggaca
    43381 tttagtttta agtacattac atgcaaatac agcagttgga gcaatttcac gtcttttaga
    43441 tatggatgtg gaaagttttt taataacttc atctgtttta ggtgtaatat ctcaaagact
    43501 tacacgaaaa atatgtgagc attgtaaggt ttcatatgaa gcagatattg gagaaaaaaa
    43561 ggctcttggt attgatgtaa atgaatcagt aactatttat agaggaaaag gttgtgaaag
    43621 gtgtaataat actggatata aaggacgtct aggtatattt gaaatgcttg aaattactcc
    43681 agaaataaaa gagttaatag attcatctgc aaatcaaaga gaaattttaa agatggctag
    43741 aaaacaaggt atggttagct taaaagagga tatagttaaa aaagttttaa atggaaagac
    43801 aacagtagaa gaaatgatta gaataatttt gatgactgat taaaggatga tattatgaaa
    43861 agttttaaat acacagtaat aaaagaggac ggaaaaaagc aaactgatgt gattgaagcc
    43921 aacgatttta atgaagctag aaatcttctt agaaagagaa atcttagagt tattgaaatc
    43981 aaagaaagca aaaataaaaa tataagatta tctagtagaa agagaaaaaa agacttagga
    44041 gctgaccaga taagccactt ttgtagacaa tttgctatta tagtatcttc tggtataaat
    44101 agtatttctg gattagaaac attagctaga aggtcaacta acattacatt aagagaagaa
    44161 attaatcgta tagtagcaga aataaaagta ggttctacta ttgcagattc aatgctttca
    44221 tcaaaatcaa agtttccaaa gttacttggt gctatggtag ctacaggtga agctacaggt
    44281 aaattagatg aagtattaaa aagtatggct tcattttatg caagtgaaca cagggttaaa
    44341 caaaagctta gaaatgctgc aacatatcca ttaatagttt tgatttcttc atttataatg
    44401 atttttatat ttacaacatt tatggtgcct aagatgatta attcaattac aacagtagga
    44461 gcaactcttc cacttataac aagaattgtt atggggtttg gtatgtttat gaagaagttt
    44521 tggataatag tacttttagt tattatatta tttatctatc agtttaaaaa atatctaaaa
    44581 acgtcagttg gaagggctca taaagataga gttataaata agatacctgt tttaggaaaa
    44641 ggaataaatt gtatggtagc tacacgattt tcaagagcat tgtatctatt tgtatcaaca
    44701 ggatacccat tagttcaagg gcttgattat attatagata gtgtaaataa cactatggca
    44761 gagaagattt tagcatcagc taaagatgga ataacaagag gagaaggctt agcagaaaat
    44821 ttagagagat atacgtattt tgactctgtg ttggtacaga tgatagctat aggggagcag
    44881 acaggggaat tagagaatat aagtagacag atggcagaat tttatgagta tgaatccgag
    44941 atttatttaa ataggatggc ctcaatgata gagcctgttt taataattgc agttggtatt
    45001 atagttgcga tactggttgt atcaatattc atgccaatgc ttagtatata tgatgccatg
    45061 taggagggaa aggacaaatg aacaaggttt atgtatcata ttatggcgat tctatttcca
    45121 tagttgaggg cagatataaa cacgataagg ataagtttta tgtaaaaaat tttaatgtgt
    45181 tttcaataga ggatattgca caagattttt ctaaggaaaa gtatgctcta ttgagatacg
    45241 cattagaaaa tgttaagtta aaatcaagac atgtagtttt ttgtttaaat acaagagatg
    45301 taattttaaa accccaaaaa cttcctaata tggacaaaaa ggatttagac ggatttatga
    45361 agctagagat ggacgaaatg atggctttgg agtcagatga atatgtattt tcgtatgaag
    45421 tatctagtga aacacctgat aattcagatg gcgatgaatc aagcttaaat cttattattg
    45481 gagctataca aaaggaagag gtaaatgcaa ttgtagagat aatgcatgaa tttgatttag
    45541 tattagatag aatagataca ttatcaacag cttacttaag aatgcttaaa aatttagact
    45601 acaatgatat aatggtaacc aatataagtg acaatagcac aattataaat atatataaga
    45661 aagatacttt atttattagt gataatatac ctataaagat aactgctaaa gatatggaca
    45721 tgcaattatt aaatgtagta gaagaatcaa aaggtcttat gaattactat tcgtcaagaa
    45781 actttggtaa ggttacggat actatattaa tattaggaaa aagatattcc aataaattaa
    45841 tttatgaaac ttttagctct gcattttcaa gctatgtttc tcaaggcctt actgagataa
    45901 tggatatatc tgatatgata actggagata tagatgaaga tgatataaat tcaattgtag
    45961 agcttttagg aagtatgata gaacctaaag ggaaaagaga ttttgaaaat ataaactttt
    46021 taccaatgga tatacttaga aaacagataa aagctaaaca aatgaaaaac atactgaaaa
    46081 ttgctccaat agttattttg attcttgcta taccttattt atgtttaatt ttttcaaata
    46141 atttagcaaa taaggaactt gaaaatgtta atggtgaaat aagaaatatt gaatctgcat
    46201 attatcagat tggaaatata gaaaaaatga tacagagtaa aagcgaagaa ataaaggtat
    46261 atgatatgtt aattgggaaa aaggtaaagt gggaaaattt attggatgat atagataaaa
    46321 atactccaag taaagttgaa ctaacaagtt tatcaacaac ttatcaaaat cttggaacta
    46381 ataataaagc tgataattca aaaagtaata agactacaac agataaatca agtaacaatg
    46441 aaacaaaatc aagtacgaaa agtgatagta atggagataa aacagataat gatttaaatg
    46501 aagataagac tacaaatgtg tcagaagcat taactggaaa tagtgagact aatagtaata
    46561 acacggataa agatgataaa actccattgt atgataagat accaaatgtt ataaatatag
    46621 aaggaaatgc taaagattcc tcttatgtag gtcaattttt atataaatta aagggattat
    46681 actatttcaa ggatgttaaa ttacatagca taaaaagtaa tgcagaagat tctaactata
    46741 catttagtat gacattatac ctgaaagaag gtgtattaac taatgaataa agatatattt
    46801 aaaaaagata taaaagatat atttaaaatg gaagttagtc taaaagggat tcttataaag
    46861 gttataattt tattagctat tttatcatat ggttattttg gactttaccc tcaatatcag
    46921 aaatatattg ctacaaaaaa ttctgtaaaa caggctactg aaaaattaaa tatatataaa
    46981 gagaaaactg cacttatgcc aaaatatgaa aaacaacttg aggaagttga aagtgaatta
    47041 aaagaaaaaa gcaaaaatct tttatatgat atgcaagatg gtcttttttt aataggttta
    47101 gataaaaaaa tgaaggctta taatattgac ttaaccagtt atgacgttgg aaatgttaaa
    47161 gaatacgaac atttttatgg tatatcaaca acaataagtg ttagaggtga ttatagaaat
    47221 gtcaaaaaat taatggctta tttagaagaa caaaaaaatg ttactcaaat actagattat
    47281 acaatgactt catatataga accagaaaaa gaagaggaag aaacaacaaa caataaggat
    47341 ttaattaaaa atgtgaacat aaataaagat acaaaaatat actgggtgga aggcgaaaat
    47401 aagtggcatt tatataagga ttgtccattg ataaaagagg gagagattat aaaaaatgac
    47461 aaacttttag ataggtctac taattatcta tgtaatactt gtgtacttag agctgcaaca
    47521 gaaaaagaag caaaaaaaga agtcgtagct ccaaaggcag aaggtgtagt agatgctaca
    47581 tttaaattta taatgtacac taaagagaat actacaatga tacttgatac agataatcca
    47641 tcttcatgga gaccaggaaa atataatcca tttaagtcta gtgcgaaata aagctaagta
    47701 agctttaaag ataggtgatg agtttgtaca aaaataatga aaaaggtctt actttattag
    47761 aagtaataat agctgttttt atactgacaa tagttttaag tatttcttat aaagtgttca
    47821 atggtataac atcagcagta aaaaagcaac agattattac agatgctcaa gtaaatatta
    47881 atctaattaa taagtatcta aatagagatt tggaaaactg taaagagcta actaaaactg
    47941 gttcaggtaa taattatgaa tacaatatag agatgccaga taatgttgta aaatatgaag
    48001 tttctataga aactaaaaaa aatacagagg tatattctgt aacgagaatc caaaacaata
    48061 caattgatac agaaaatgaa gttagagaag agataatcta taatcaacca ctagttcaaa
    48121 ataataaaga aatgaaggaa acaccattta aaatagaaaa gcaaactggt aaatctattt
    48181 atactgtaag tatatactat aatgaatcgg tgcaagaaag tcataaaaat agcaatttaa
    48241 ataacaaaac ttatacattt gatgttatgt caagaatagg gtaataagaa tttattaaaa
    48301 aataagtagg ttgataaaat atgagtaaaa agaatagtga aagaggattt tccttaattg
    48361 aagtgttagt agctatggct attatgggaa ttgtactatt tgcatttttt aatattatca
    48421 atactaataa caaagcaaac actaaaaatg acacagatat aacttcctta aactatgtcc
    48481 aaagtgaaat agaaaatcta agagaaaaga taaaaagtgg agaatttgat tttgatagtt
    48541 tagataaact agaagatgga actgtcgtat atgaaaaatt aatagataaa tcaaaaaaag
    48601 ttgtatatga taaagttctt agtgaaggtg atgtgagctt atatgatact ccatatgaaa
    48661 agattacaac aataaaagat gaagatggta atttaatcga caaaggaaat attacaaata
    48721 agataaagac tattgtagaa gataaaagtg gtcagatata taaaatagct gtaactggga
    48781 aaagcatgaa tgactattca agtaaaaaag aggttaaaat tgtaactgaa atatttaaag
    48841 ataaataatt ttacttgggg gtgagatttt gaggaagtgg aataaattta aaagtgaaag
    48901 aggggcagct ttagttttag ttttggttgt agtagcattg ctttctattg ttggattgat
    48961 attttcaaat cagatagcta atagaataaa atctacaaag actactaatg aaggtataca
    49021 agcaaagtat ttagcagaga catgtgtgga aaatagtatt gataaagcat atgaaaaatt
    49081 gtatgatgag cttgagaaaa tggataatga gtttaaaagt gaaaatcaag aaaaatccat
    49141 atctaggtca aaattaagaa atatatcaga tgaagatttt aataatcaag atgaggaaaa
    49201 tattgaagca gaaagacttg gatatatgaa taatataaat ttctatttaa ataaagcttc
    49261 ttcagacttg gaaaaagcta gtatggaatt gaaaaagcta tatgatttag atatgttgga
    49321 ctatagagac attgaatatg tagatgcaaa tattatttca catagaaatt ctatcttaga
    49381 aatttgcaag aattatactt ctggagatat tagtaaaata aatgaatata ttctgaaaga
    49441 agatatagac tcaactacat tgattgaggc aaagttagtg aataatgata ttcttttaaa
    49501 aaagttttta gaagaaaata aaattgaaaa tgaacattta aattcagcct tttcacatac
    49561 ttataaggca ttagataaca tttctttggc gatgcaaaat atgattgagt atagacatac
    49621 gtttcatata gatgagccaa aagtagaagt atctaatggg attccagact ctcaacaata
    49681 ttatgaacta atacaaaatc ctataataaa tagtatggag tatagctgga atagtaaatg
    49741 ggatacatta gaaaacctat tggaaatttt accaaatcaa actcaaggat ttaattcttt
    49801 aagagttcat ttgagaaata atgtaagaaa atttgagaag ttaagcgata atatatctag
    49861 tggtaaaaaa aatacagcaa aaaatttctt aaagtataaa gagttattgt atgaaatatc
    49921 agaccaatgt aatcaactaa aaagtatgtc ttatgagaaa atacctgtta aatatgataa
    49981 catggctttg ataacaactt ttgactatat acaaaatgag cttttagcag agataaaatg
    50041 cagattaaaa gaattaaaac cacaagaaat tgataaaaca gaaggtatta ctataaaaat
    50101 acctttttat aaggctgact acgacatgac aaaagaagga tggcctaaat taaaagaaaa
    50161 tggttcaggg gcggagttaa gcttaatggt aactggtgat aaaaatggaa taaaagaagt
    50221 agaagttaca gatggtaaaa aaaatattat tggtttaggt gttgaagaaa attcaaattc
    50281 aaagtataag gttgacgcca ttgtaaactt taatctaaat atagatacaa atgtagttgg
    50341 aaattatgat ataaaagata aaattttgat taatcatgat ataagttcat acaaaaaagt
    50401 aaactaaata taagaagcaa cttagtataa ataaataata aaataatttt attatttact
    50461 actaaaaaaa taagtaaact aatagaagat atggagagta aatattatgt atatttatga
    50521 tttattagag caaggtataa gactaaacgc atcagatata cacataacag ttggaacaaa
    50581 tcctgtagct agagtaaaag gtggatttgt aaaactaagt gaacaaatat tgacatcaga
    50641 agtaacaatg caaatggcaa aagacatagc aggggaatct atgtttaaag taatagaaga
    50701 acatggagaa gctgattttt cagcttctct aaaaacagga gaaagattta gggtaaatgc
    50761 atataggcaa aaaggaaatt acgcaatagc aataaggact ataacagcag aaattccaac
    50821 atttgaaaaa ttaggccttc cagagagtat aaaatctttt actgaaaagc ataaaggatt
    50881 agttttagtt acaggtccaa caggaagtgg taaaagtaca acacttgcaa gtatgataaa
    50941 tataataaat gaaaaacaac aaaaacatat aattacactt gaagacccga tagaatatgt
    51001 acatcatcat aaacaaagtt tagtaaatca aagagaagta ggaactgata cagaaagttt
    51061 tcattcagcc cttagagcta ttttgcgtca ggacccagat gtaatactaa taggagagat
    51121 gagagaccct gaaactgttt caatagcatt aacagcagca gaaacaggtc atttagtatt
    51181 ttcaacacta catacagtag gggcagctaa gacaattgat agaatagtag acatgtttca
    51241 accatcccaa cagcaacaaa taaaaacaca gttatctaca gtttgtgaag gtgtggtatc
    51301 acagcagtta cttcctacag cagatgggaa aggcagaata gcagcaattg aattgatgtt
    51361 tgctactcca gcaataaaaa acctgataag agaaggcaaa acatatcaaa ttccaaacat
    51421 gatacaaact ggagttaaag taggtatgaa aactatggac caagatttaa tggaacttta
    51481 taaaaatgga aagattacaa aagatatggc acttagtagg tgtactgacc aagagtttat
    51541 gacaagaatg ataggtggag taaactacaa tggatattat aatcgttaat ctatatgtat
    51601 ttatagtggg tatagtgttt ggaagtttct ttaacgtctg tatttttagg attccagaag
    51661 gtatatctat ttctagacca ccttctcatt gtatgagttg taatactaga ctaaagccaa
    51721 tagacttaat accagtgcta agctatttaa tgagtggcag aaagtgtaga tattgttatg
    51781 agaaaatttc tagtagatat gctatagtgg aattaattac aggactttta tttttaacag
    51841 tatttatgtt atatggaata tctacatcaa ctatttatta tttggttctt gtgtctttac
    51901 tgattataat tacatttata gatattgacc actttataat accagataaa atattggttt
    51961 ttggagctat attttctatg atttttaacc ttttatttaa agaaattcca gttaaggatt
    52021 caattctagg tgtttttatt tgtggaggaa gtgtctggat tatagttctt ttgattgaat
    52081 ttgtaataaa aaaagagtgt atgggtggag gagatatcaa actatttgca atgctaggtt
    52141 tttacatggg tgttaaaaat ggtcttttga cagccttact aagcgtttat gtgggagcag
    52201 tctatggaat ctgtgtaata attataagta gaataaaagg aaaggaatat aattctgtta
    52261 taccatatgg accttttata tctattggag cattaataac aatcttatct ggaaagcaat
    52321 tacttgaact ttatttttct atggttatat agatgatagt caagttaaat aaagttttaa
    52381 tttaaaaaaa tgaattaaag tatttaataa ggacaatatt attattaacc ctattactaa
    52441 agtgctacaa actatggagt atataataat aatttcctta ttttttgcac atttgataaa
    52501 taaatctcta tgtgtttttt gtgatagaga aaagaaaaat ttatataata cgttttttat
    52561 ttacataata actgtagttt tatctgtatt aatatatcta aaatttggga ttagtgtaga
    52621 atgtgtacaa tatatggttt taattccatt tttaataatt atatccattg tagattacta
    52681 cacaatatat gtatatgata taacaatagt tagtggtata atagttcaag gtataatttt
    52741 tttggccttt ttcaatatag ttcaatttga ttatataaat catatatttg gactattgat
    52801 aggctttatt ataccttata ttattgctaa actaacaaaa ggattaggct atggagatgt
    52861 aggtctctat agcttatgtt gctttgcatt gggaaataat tatgggtttt atgtgatagt
    52921 tttatcattt attttagcgt ttttgttttt tattatttta tacataacta aaatatttaa
    52981 agatgtagat ataagaaagc aaattccttt tacacctttt atttccttgt caacaatttt
    53041 aataatattt acagagtata atatatttaa tttttattat aatttgttat acaaatttat
    53101 ataacaggag gtaagaaatg tacgtagtag tagggttagg taatccagga aagaagtatg
    53161 agaagactag acataacgtt ggatttgatg ttatagatat tcttgctaaa gaatacaata
    53221 ttagtgttac taaaataaag cataaagctt tgattggtga agggagagta ggtactgaaa
    53281 aagtattatt agttaaacca caaacttata tgaatttaag tggagaaaca ttaatagata
    53341 tatataaata ttataaggtt gatttaagta atatagtagt agtatatgat gatatagatt
    53401 tagaagttgg aaagatacgt ataagaaaaa aaggaagtgg aggaactcat aatggcatga
    53461 aatctataac taagtgccta ggttcaaatg actttccaag agtgagagta ggtgtatcta
    53521 aaccagaagc tggtcaagac ttagcagact ttgtactttc tagatttaga aaagaagaat
    53581 ctgataacat aaatgaagca ttagaaaaag cggcagatgc tattgactca ataataagag
    53641 aaaatataga tatgtctatg aacaaatata atggatagtg agagggagat ttaaatgaat
    53701 gatgtttttt tatatccttt gcaaaattca aaagagtata aagatataat aaactgtata
    53761 aaaaatacaa aaggctcatt attggttaat ggacttttac cagtacaaaa acctcatata
    53821 tcgtattcta tatttaatga tttatcacga caaatgattt ttataacgag tagtgattta
    53881 gaagcaaaga aagtatacga agatttaagt ttttatatgg aagataaagt tgaatattta
    53941 ggttttcaag atatttattt ttatcacttg gatgcaaagg acagaaacga agaagcgaag
    54001 aaattaaaag tattgttaaa actagcaaat aaagaaaaaa tcattttagt aacttctatt
    54061 gaggcagttt taagaaaata cataccaaaa caagttttgc tcgatagtgt atctcattat
    54121 aaggtgggag atagcttaga cttagaaaag ttaactgaga aattagtttc attgggatat
    54181 gaaagagttt caaaaataga ggggtttggt caatttagta taagaggtgg aattattgat
    54241 gtattttcac ttgaatatac aaatccaatt cgtatggaat tatttgatga tgagatagat
    54301 tccatacgaa cttttgacgt atattcacaa aaatcaattg ataaattgca acaattctct
    54361 ataactccat ctagggaatt tatttatccc gaaaaaacta cagatgcatt agttaaatta
    54421 aaaaaagaaa caactaaaaa tactgatgaa ggtacatttc aaaatataga ttatatatca
    54481 agtaagactt attttgaagg tgtagagaac tatatagatt atatttatcc agaagaaaac
    54541 aaaagtatat ttacttattt agcagatgat gcaatagtat ttattaatga tataacgaga
    54601 cttaaagaaa gatgcgaaaa ttatataaat gagtttagag aaaattataa attaaatctt
    54661 gaaagaggtc tggctattaa aaaccaaggt aaattgttat atcactatac agatttagag
    54721 tacttagtaa aagacaagtc tgtggtatta aactcgctat tgccaaagtc tataaataac
    54781 ttttctataa aatctataat aaattttgaa agtagagaag ttcctacatt taatggaaag
    54841 gtagatttgc ttgtagaaga attaaatcgg ctgaaatata atggatacaa aataatttta
    54901 gcaacaaata ccttagaaag agcaaataaa ttaggaaaag atttattgga caaaggctta
    54961 gaaacgacca tttctaaaga tagagatatt gaaattaaat cttcacaagt tataattgta
    55021 cctgcacaca taaacagtgg ttttcagtat aaatctataa agtttgttgt tataacagat
    55081 aacgaaatga taggtgttta taaaagagcg tctaagacta gcaataaaaa agttaaaaag
    55141 ggaaagaaaa tagaatcctt tttagattta agtgttggag actatgtagt ccatgaaaat
    55201 agtggtgtag gtagatacac aggaattgaa caaattacag tcaatgctat taaaaaagat
    55261 tatatgaaaa tagtatatca aggtggagat aatctatatg tgccaataga ccagatggac
    55321 aaagtacaaa aatatattgg tgcagaagta gaaaaagtta agttaaataa gcttggaaca
    55381 aatgagtgga cgaaagcaaa agcaaaagtt aaaaaagaaa ttgaagatat gacaaaagac
    55441 ttgattgagc tttatgcaaa acgtgaaaaa atacaagggt ataagttttc aaaagataca
    55501 ccttggcagg cagagtttga atcgttgttt ccatatcaag aaacagaaga ccaattaaag
    55561 gctatagaag aaactaaaaa agatatggaa tcaagtaagg taatggatag gctagtatgt
    55621 ggagatgttg gttatggaaa aacagaggta gctattagaa gtatttttaa agcttgtatg
    55681 gaccaaaaac aagttgctgt attggttcca acaacaatat tggcccaaca acattataat
    55741 acattcaaag agaggtttga aaattatcct ttgagagtag aagttttaag taggtttaaa
    55801 acaccaaaac aacaaaaaca gattattgaa gatgctaaaa aaggattagt agatatttta
    55861 attggaacac atagaataat atccaaagat ataaatttac caaacttagg tcttgtagtc
    55921 attgatgaag aacaaagatt tggagtaaaa cataaagaag ctttaaagaa aattaaatct
    55981 acagtagatg tacttacact ttcagcaact cctataccaa gaacattaca tatgtcttta
    56041 agtggaataa gagacatgag tgttatagag gaacctccac aagaaagaca tcctgtaata
    56101 acatatgtta cagagagtaa ggaaagcgta attcaagatg agatagagag agaattaagt
    56161 agaggtggac aggtattttt tgtatacaat agagttgaac acattgaaga aatggcaagt
    56221 atgattcaaa aactcgtacc agatgctaga gtggctgttg cacatggaag aatgacttct
    56281 aaatcattag aaaatataat cctaggattt ttaaataaag attatgatgt actagtctgt
    56341 actacaataa ttgaaactgg tatggacata tcaaatgcaa atacaatgat tatatatgat
    56401 gcagataaaa tgggattggc tcaactttac caattgagag gtagggttgg aagaagttca
    56461 agacaaggat atgcatatct tttgtatgaa aaagataaaa ctttaagtga gatagcggag
    56521 aagagattaa aagctatcag agaatttaca gagtttggtt ctggttttaa aatagcaatg
    56581 agagaccttg aaataagagg agcaggaaat attttaggtt ctcaacaaca tggtcatatg
    56641 gcagttatag gttatgattt gtacgttaaa atgcttaatg atgcaattaa aaaagtcaaa
    56701 ggtgaaccta ttgtggagga aattgatgtt gaaatagatt taagtgtaaa tgcatacatt
    56761 ccagataatt atatcaaaga tgagttgatt aaaattgaaa tgtataagaa aatagcttct
    56821 attgaaaaca aagaagacat gttggatata caagaagaac ttgaggatag attctctgat
    56881 atacctaaac cagtccagac acttcttaca atagcttata taaaatcgtt atgtaagata
    56941 ttaaaaatag aaaagataag acaattaaaa gatgaaatat tattagttcc aataacaaag
    57001 tatagaacta aacagaaaat aggttataat atagttacag aattagaaga attattagaa
    57061 aagatgtgca aggtaaaata acttgtaaat ttaagaaggg atgttttaaa tttgaaaaag
    57121 gtaataacat tggtaatagc tatgattttg gtagtatctg tgactgcttg cagttctagt
    57181 aaaggagaaa ctgtggcaac agttgaaggt acaaagatat catcagatga atttaaaaaa
    57241 actatagctc tttataaaga ttctatggaa caaacttatg gaaaagatat atgggataag
    57301 gaagttgaaa agggtgtaaa atacaaggac aagtttaaag atttaattct tgaccaactt
    57361 ataactacag aagtaatata ttctcaagct aaaaaagata acttacttcc taaaaaagag
    57421 gatgttgaaa aaagctttaa agaattaaaa gacgctatgg gtaaagatga aaaatataaa
    57481 gaacaactta agaaattagg tatagatgat gagtttttaa gagaccaaca agagaaagat
    57541 ttagcaatgc aaaactatca aagtaacttt gctaaaaaga ctaaaatatc agatgaagaa
    57601 atgaaaaaat actatgatac acataaagat gaatttaaaa aagatgaggt agaagcttct
    57661 catattttat tgaaaacagt agatgataat aacaagccac tttcagataa ggaaaaagct
    57721 gaagctaaga aaaaggctga agaggctctt aaagaagtta aatcaggtga ggattttgca
    57781 aaagtagcaa aaaaatattc acaagatact tcagcaagtg atggtggtaa attaggattc
    57841 ttttcaaggg gtcaaatggt tgctgaattt gaagatgctg ctttttctat gaaaaagggt
    57901 gaagtatctg atttagttga aactcaatat ggataccaca taattaaagt gacagacaga
    57961 ataaatgagc aaacatcatt tgaagatgct aaagaaacta taaaagacca actgttgaaa
    58021 aataaatatc aagaacaaat agaaaaatta actaaagaag ctaaggtaga aaaagatgaa
    58081 aaagtaataa ataaaatcac aatctaagac ttaaaaaaag agtaatatgc ttaaaaaagc
    58141 tatatctgga taagatatag ctttttttgt atatttttct taacaatggt aaaaataaac
    58201 ttaaagtaat ttatggaggg attaaaaaat atgaaagcaa caggtatagt tagaagaata
    58261 gatgatcttg gaagggtagt tatcccaaaa gagataagaa agacattaag aattagagaa
    58321 ggagaccctt tagagatatt cacagcaaaa gatggagaag tcatacttaa aaaatactct
    58381 ccaataggtg aattaaatga attttcgcaa gaatatgctg aaacattagc tgaagtacta
    58441 ggatatggag tgttgataac agacttagat tctataatag ctgtgtctaa gcttcctaaa
    58501 aaagattata aagaaaaaag tataagtgat gagttagaag caataataag tgaaagaaca
    58561 gtaaaatatt caaaagataa aaacatttta ccattattca aggaagacca aaaagaatat
    58621 actagtcaag ttatcatgcc tataattagt gcatctggtg actgtatagg ttcaattgta
    58681 gttgtttcaa aggataaaga tacaattgca gaaagtgaag aaaaatcttt aaaaattgct
    58741 gctaactttt taggaaaaca agttcaataa ttttatcata aagacctttt acatgaaggt
    58801 cttttgaagt ttttgtgtaa aatattgaat atagcattaa aaatatggta tattaaagtt
    58861 tgttatataa acagtatgga ggttatgtta tgaacaataa caaaaataaa gactcctttc
    58921 taaaaggagc ccttatactt gggctagcag gtgtaatcgt aaaaataatg ggtggatttt
    58981 ttaggattcc ccttggaaac atgattggtt cagaaggaat gggatactat caggctgctt
    59041 atcctgtgta tactctattt ttaacattag ctactgctgg atttccaaca gctgtggcaa
    59101 aattggtttc ggaaaaagtt gcaattggaa attttaaagg tgcaaataag atatttaagg
    59161 tatcacatac agttttattc attaccggta taatatcatt ttgtatatta ttttttggag
    59221 ccgactacat agtaacaaac gtaatgaaaa atccaggtgc gttatactct atgaaagcca
    59281 ttgctccagc attactattt gttcctgcaa tgtctgcata tagaggatat tttcaaggga
    59341 gacaagatat gactaagatt gctgtgtctc aagttgcaga gcaatttttt agagttgtat
    59401 taggtcttac tttggcttac ttgcttatga agagtctagg tcagactttt ggtgcagcag
    59461 gtgctatatc aggggctaca ataggttcaa tagcatctat gctttatttg gtgtttgctt
    59521 atatgctagg gagaaaagag cgtagagcag agatagatgc aagccaaaga tttaaagatg
    59581 aaagagtgtc ttatattttt aaaaagcttt taactgttgc cattcctata acaataggag
    59641 catcagtaat gccacttgta aatatgatag ataatgttat tgtaataaga agacttatgg
    59701 aagcaggctt tacatacaaa gtggcaaatt ccatgtttgg tcaattaaca ggtatggcaa
    59761 tggccacaat aaatttacca gcagtcataa caactgcaat gagtatgagc ttagttccag
    59821 caatttctaa agcatatgca ttaggaaata agtcaaaagc tagaaaagat actaagagtg
    59881 cagttaaggt aactttactt atagtattgc cttgtgcgtt tggtatggct tctttagcaa
    59941 taccaataat gggtctttta tttccacatg aaccatcttc tgtaggaact attttgttta
    60001 ctcttactcc atgtgtatta tttttaggat taatacagac attgactggt ataattcagg
    60061 gaatgggaaa accaatagta cctgtaattg ctttatgtgt tggtatgcta tgtaaaattg
    60121 taataagtta tactttaaca ggtataccag atattaatgt actgggttca gcatttggta
    60181 cagttacagc atactttgtg gcagccatga ttaacttact atatgttaaa aaacacatga
    60241 atgtaaactt ttctaaaaaa gaatttataa taaagccatt tataacagtt atgacaatgt
    60301 ttataatggt taagttgagt tatggtgctt tagttggatt cttaggaaac agcatatcta
    60361 caataattgc aatatgtgtt ggtggaattg tatatgtagt agtaatactt ggaataggtg
    60421 gaattaaaaa agaagagatt ctaactatgc caaaaggtga taaattatat aaattgttaa
    60481 aaaaagttaa acttataaga taatatagga atatatatgc aaataaagga aatactatac
    60541 aaatagtaaa tctccttatt tgtataataa tataaaaata aagtgaaatt tgctaatagg
    60601 agtgatgtta tgggtaagat aagtattgta ggtcttggac caggagacta ttctcttata
    60661 agccaaggtg tgttagaatc tttaataaat agccctaaag ttttttttag aacagagaaa
    60721 catccaataa ttgataaact taaagaaact atacaataca cttctttaga ctacttttat
    60781 gaaaaagagg aaaattttga taatgtttat ttgaaaatat ctgaatttat catagaagaa
    60841 tcagcaaagg gtgacctagt gtatgctgtt cctggtcatc caagagttgc agagaagact
    60901 gtaagtataa tagagagatt agccaatgaa aagcacatag cagtagaaat attgccttct
    60961 atgagctttg tagacgctat gtttaactat ttggctatag acccatctga gggatttaaa
    61021 ctggtagatg cttttgaaat agaaaattca tatatagact tggatacaag tatgataata
    61081 actcaggtat atgataaatt tatagcatct aatgtaaagt taaaacttat ggaacattat
    61141 gactataatc aagaagtatg tatagtaaat ggagctggtg tcaaagattt agaaagtaaa
    61201 aagtttataa agctttgtga attagacaga aatgaaaact tttttgacta tttaactagc
    61261 ttatatatac ccaaaagttc taaaaagatg tataatacag tacatgactt agaaataata
    61321 gttaatacac ttagaagtcc atctggatgt gaatgggata aaaaacaaac acatcaatca
    61381 ttaaaaaatt ctgttataga ggaagcatat gaactttgta atgcaattga taataatgat
    61441 atagatgaaa tggtagaaga gttaggtgat gttcttttac aggttatatt ccattgtcag
    61501 ataggaaatg aggagggcta ctttgattta aaagaagtag taaatggcat atgtaaaaaa
    61561 ttaattcata gacatcctca tatatttagt ggtgcaaaat tagatatgtc taagtttgag
    61621 aaaacttggg aagaattaaa gaaagaagag aaaggcgaaa gtactataac agatggcctt
    61681 agaaggatac caaaacattt accagctctt atgaaggcta gtaaagttca acataaagca
    61741 gcacttgtcg gctttgattg ggacaatgta gaagatgtat ttaaaaaaat tgaagaagaa
    61801 tatcaggaat tacttgacga acacagggtc gggaatataa aatacataaa ggaagagcta
    61861 ggcgatttat tattttcgat agtaaatcta gctagatttt tagacataga ctcaggagaa
    61921 gccttgaact gtactacaga aaaattcata aatagatttg actttataga aaaaagtgca
    61981 atgaacttga ataaaaaatt agaagacatg acacttgaag agatggatga gttttggaat
    62041 caggctaaaa aacaataaaa ttgtggaagg aaatttgtca aaaatctaga acttttatca
    62101 caaagataga agtctatatt ataaacattt ttaggaggta aatatcgtga ataaagctga
    62161 attagtatca aagatggcag aaaaaagtgg attaacaaag aaggaagcag aagctgcgtt
    62221 aaacgcattt atgagttctg ttcaagatgc actagtaaat aatgaaaaag ttcaattagt
    62281 tggatttgga acatttgaga caagagaaag agctgctaga caaggaagaa atccaagaga
    62341 tccagagcaa gttatagata taccagcttc taaagcacca gttttcaaag ctggaaaagg
    62401 attaaaggat ataataaatg gataattaga aatcttgtga aaaagtgtgg ataaatttgc
    62461 cacacttttt ctatagaacc aaggaaggtg ataattatga gattagataa atttttaaaa
    62521 gtatctagga ttataaaaag aagaactgtt gctaaagatg catgtgataa agggattgtc
    62581 actataaatg gaaaagttgc taaatcttct tcagaggtga atattggaga tacattagaa
    62641 atacaatttg gagagaagaa gatgaagttt aagataaatg aagtaaaaga gcatgtatta
    62701 aaaaatgatg caaaagaaat gtacaaaatt ttagaataaa attaatagtc ctctagtata
    62761 ttaatagtag tattattgaa atatttactg gggggatatt atggaacaga acataacttt
    62821 gaaagataga tcaaagctgg ttatttcggg agtcgagcac atatactctt ttaatgacaa
    62881 aagagtagag ctcaaaactt ctgtaggcga gatggtaatt gaaggcgaaa acctagatat
    62941 gagcaagcta agtatagacg aaaatataat tagcatagat ggaactataa attctatggt
    63001 atatgcaaag cctaaaaagc cacaagagag tttcttgaaa aaggtattta gatagatgat
    63061 accttttact caagatgtaa gcatttttta tgctactatt tatggtggaa tactcatagg
    63121 ggtgttgttt gatttttata gagggctaag agggaatttt aaattcatca attattttgc
    63181 tataatcttt gatgtattgt tttggtttct agctacggtt ataatattcg taacaataaa
    63241 ccttacagaa ttttttgatt taagatatta tcattttgtg gctttgttta ttggttttat
    63301 tttatactat aacaccataa gtaaaattgt tttatcaatt atcaataaaa taattcgttt
    63361 tgtgagaaat tcttttaaaa aagttacaca ttacatagtc agttttttaa ataatttgta
    63421 ttacgttata atctattcac tacacctact atttgatatt atattttata taccaaacat
    63481 atttatagca acaagaaaaa gtataaaaag aaaatcaaat aaaaaattaa agaacaagaa
    63541 aaaatcaaaa cctaagaaga aaaaaaataa aactaaaaaa agggtgtagg aaaaatgaac
    63601 ttaagaaaaa aattttcagg acagattatt gttatcagtt tatttttagg aatatcaatt
    63661 tttagtatga tgacaggatt tgtatttgag tatacaaaag ctaaggaata taaaaaagaa
    63721 atagcaagtc taaataaaca attaaaaaag actgaaatac aaataaacag cttaaagaaa
    63781 gatgaaaaat catatgaagg agacttagag gatatagcaa gaaaaagatt gaatatggta
    63841 aaaccaaatg aaacagttta tgtagacata aatcgttgat taaaacttgc taataagttt
    63901 acagatgtat tgattttaaa atttaagatt taataaaaaa gtcattcagt taaggatggc
    63961 tttttgtttt tattgaggag ggaatttcat aatgaaagta ggagctatag atattgggac
    64021 aaactcaatg agattattga tagcagatta tagagatggg aattttataa acagaaaaaa
    64081 atttgttaat acaactagaa ttggttatgg tgtggatagt aaaggatata taaaagaaga
    64141 tgctatcaaa agaaatataa aagcattaaa agaattttct gataagtgta atgatgaaaa
    64201 gtgtgaaaaa atttattgta tggggacatc tgctcttaga gacagtaaaa atggagatta
    64261 ctttgttaaa ttagctaaag aagaaactgg aatagatgtt gatatagtta cgggaataga
    64321 agaatcaaat ttgggcttta agggtgtttt agaaggaata caaggtgatg atactattat
    64381 tgttatagat attggaggag gttcaacaga atttattgta ggtgattcag aaggaattaa
    64441 atttaataaa agtgagaatg taggtgctct taggatgaca gagaggtttt tgaagcacga
    64501 tattattgaa gatgaagagt ttgaagctat gagttcattt atatatgatg agattgacaa
    64561 aacgttaaat catataaaag acagaggatt aaaaactctt ataggtatag gaggtacgat
    64621 tacatcatta tcagcaatga atcaagaatt agagatttac tctatggaaa aaatacataa
    64681 tagtaaaata cacaaaaaag aaatagaaga aattctacaa aaattaataa agatgacatt
    64741 aagtgataaa aagaacttaa aagggctaca agaaaaacgt gcggatatta taactgctgg
    64801 tgtaaaaata ttgaatataa taatggaaaa attagaaata gaagaaataa ccgtaagtga
    64861 atacgataat ttggaaggct taatatgtca aaagtcaaaa aaaatgtatt aatatttttt
    64921 gatttttaaa accgattatg acaaaaaaaa ttactcctct ttgttacaat tcttaatata
    64981 aacaaaagag gaggtttttt tatgcaaaga agtgtagcag ttgtgaagaa taagactgta
    65041 aaaccaaatg tcataaaatt aataagcgga tatattgatt ttactacggc ggcattcgct
    65101 gtgatgggat ttttacttag taggtctatt atagtagact ctgtagctcc tttaggaatt
    65161 gcctttttta tatgtgcatc aaagattgat aagtacaaga tacctgtatt catatcaaca
    65221 ttattaggaa tattgttatc atctaatgat tttggaaatg tagcaaaata tgctgtgtgc
    65281 cttacaatat ttatggctat gaataaaaaa ttaaaattat cagattcaat tgctaaggtt
    65341 gcatttatag gtgcaataat actacttcca atctccatgg gacaagcttt cttttcaaat
    65401 aggtatgtat atggtcttct tatagcagga gtagaagcaa taataatgtt tatatctata
    65461 tatgtgtttt cttatgggat aggtttaatg gccaatgtca acaataggat gtctgtacgg
    65521 acagaagaag ccatatcaat aagtttactg ctaaccttta gcataatggg aataggagat
    65581 atatctctgt ttggaatatc aataaggacc attttatcga ccgtattgat attgctgacc
    65641 tcaatagtag gtggagctac catgggagca tctagtggtg taattattgg tgtagcatcc
    65701 attttaaata atatgacatc agctgtttat atggggatat attctttttc aggattaata
    65761 agtggagcat ttaataaaat aaataaatat ttctgtatac taggatatat tctaagttgg
    65821 acaataatat acctatatac ttctggtatt acttctaata tgatgcaatt aagagatata
    65881 ctattaggtt gtttgatagt actagtattg ccagagagat tatttaataa gattgaaaaa
    65941 ttaataaaaa gtaatgtggc ttcaaatgaa atcgtatacg attatattat gagaagcaaa
    66001 aacctaacta atagcagatt gaacagtatt tataaaactt atgatgacct tgcagataca
    66061 tttgataaga taagagaaaa agacaaagtg ctagaccaaa gagacatagc taatgtaata
    66121 gatatgattc ataatgatga atgtaaaagt tgtagtatga gaagaatgtg ctgggagtca
    66181 agatttaatc atacatacac tatgatatat aacattcttg aaaaaattga ggagaaaggg
    66241 gagttaagtc taaacgatat acctaaaaat tttagaaagg aatgtatgaa accagagtca
    66301 atagtgaaaa tatcaaatca ctattacaaa atgtttgtat tagattatga ttggagtgtg
    66361 aagttttcag aaagtagaaa gttaatagca aatcagataa gaagtatttc aaaatccatt
    66421 aaatccttat ctcaagattt agaaggcgac ataatgctag atatagaaaa ggaaaaaaat
    66481 atatatgaac agttagaaag atatgatata acagttgata aagtaagtta cttaacaaaa
    66541 agtaacagtg agtttgaaat atcaatagaa aagaaaactt gccatgatgg atgtatgtgt
    66601 gaagataaaa ttgtaaatat aatttcagac ctagtagggg aaaatatgtc tgttagaaaa
    66661 ataggatgtc actgtttagg aggtaaatgc aaggcaacat tcgttaaatc tcaaaaatat
    66721 aaggctgtta cagaagtttc agcaatgtca agggatgggc atatcctttg tggagacaat
    66781 tacacataca tggagataaa tgatggtaaa tacatgatgg ctataagtga tggtatggga
    66841 aagggtaaaa aagcatatga agaatcttct gccactattg atatactgga aaaaatgata
    66901 gatgcaaaaa ttaaagatga gatagtcatt gatactataa acaatatgtt gttgttaaaa
    66961 tcttcagaag agatgttttc aactctggat ttgggaattt tagatttaaa acgaggttgt
    67021 ttagaaacta taaagatggg agcgtgttct acttatataa aaagagagga tggagaagtt
    67081 gacttaatat catcatcatc attacctgtg ggtatattat cagatgtaaa aattgataga
    67141 aaaaatgtaa aagttaagga aggagactat gttataatgg tttctgatgg aattgtagat
    67201 gcaggaagaa ataacaattt aggagacaat tggttaatat acttcttaaa gaatatagag
    67261 actacaaatc caaaggaaat ttcaaattta atattggata gagcattaga gttacaagcg
    67321 ttacaaatcg aagatgacat gactgtttta gttacgaaaa tatgtacaaa ataaggttta
    67381 caagattatg tgtataagat gtggataact tgtgtataat atgttaatag acttttgaaa
    67441 aaagtatagt tattaacaaa aataacatat aaatcaacaa aatttgttga taagtatggc
    67501 agaaatgtta ataaaatgta aaaaaagaaa aaacaaaagc tattatcaaa agagattcat
    67561 gttgaaataa ctaaatttct aagtaaagaa aacctgtgaa tatgttgatt attatgtgga
    67621 taactattaa ttcgatattt atttatatag tatgaataat tgaataatat atctaaaaaa
    67681 agccatccta ttattaagta aacttattga aaggatggct ttttatgaat agaaaattgt
    67741 gcaaagcaat aataccttta tttatttcta ttttttgcat gttaataata agtataaaag
    67801 ctgaccaagg atatcaaaag aataatgaca gagcaattac tgtcatgaat aataatgata
    67861 ctgaagatga tgctgaaaaa aaacaagaaa tagactatga aaaaacatgt tggaatttgc
    67921 aatctttgtt taaaagtgat gaccaatgga aaaaagaact taagagtttt gataaagata
    67981 ctaaagagtt gaaaaattac ataggtaaag taacaaaatc gccaacacat ttgtcttttg
    68041 ccttgaacat taaagaaaaa cttgatataa gactaaataa actttctgcc tatgtaaaat
    68101 taaaacaaga tactaataaa aattcgtata agtacttaga tatgaatgat agtatgagta
    68161 aatcctatgg gaattatatg gggatttgtt cagatttaga gttagaaata ctaaaattat
    68221 caaacaaaga ttacaagaaa attatttcaa ataaaaatat aaataggaaa tatggtgcat
    68281 atttaagaga tataagaagg aataaagtac attatttgga tgataaatca gaagatattc
    68341 taagcaaagt atcttcaata agtagtcttc caagtcaagt gtatgaatta tttagaaata
    68401 tggatagaaa aactaattta acaccagctc aatatgcgag tgaattagag tcatcagata
    68461 gagaaaatag aaaaaaagct tatcaagatg aacttataac ttacaatgat aatataaata
    68521 caattgcagg tttaattact ggtcaagtta aaaagaatat tttttattct caggaaagag
    68581 gttatgagtc ttctttagaa atgtacctgg aagctgataa tgtagatgaa aaagtataca
    68641 ataatttgat aaggactgta aatagtaata tggacagtct acacaaatat atagatttaa
    68701 gaaaaaaagt attaaaatta gataaggttc attcatatga tatgtttgtg cctatggtta
    68761 aggctgttga taataatatt aattatgaaa aggcacaaag tatagtatat tcagcgttgt
    68821 cacctttagg aaaagaatac aatgatgtag tatataaagc tttcaatgaa aaatggatag
    68881 atgtttattc acatgaaaat aaagtaagtg gtggatactg cttatctgtt tatgaaaatc
    68941 atccatatat acttttaaat tataataatt cattaggttc agtttcgact ttatcacatg
    69001 agcttggaca tgctgtatat gaatacttaa gttctaagaa tcagaattat tttaattcaa
    69061 atccgtctat atttactcac gaagttgcat ctacaacgaa tgaagcatta ctttacgaga
    69121 tgcttataaa agaagctaaa ggcaatgatg aaaaagctta ttatataaca cagtatttag
    69181 acttgataaa agatactctt tatacacaaa ctatgtatgc tgaatttgaa aaaactatac
    69241 acgagttagt agaaaaagga aaaagtgtaa acgcattagt attaaatgat gtttggggac
    69301 aacttcttaa aaaatattat ggacaaagct atgaattaga ccaattatct aaggtcggat
    69361 gggctagaat cccacatttt tataatagtt tctatgtcta taaatatgct acaggttgta
    69421 gtgcaggagt aagttttgcc caagacatcc taaagaatgg ttcagataac tatatgagct
    69481 ttcttaaaaa gggaagttca gattatccaa taaacttgtt aaaatcaagt ggcataaatt
    69541 taactaatac aaaacccata gaaaatacaa tcaaaaaatt tgatagttta gtagcagaac
    69601 ttgaaaagct attataaaca ttattgtaat ccacatggta aatatatcga attaatgata
    69661 aaatatacta aaagtttaaa gtcctatcaa gataggactt tttatttatt aattagtggg
    69721 agatgattct atgcaagtta aagttaaaaa ggctgtaata ccagcagcag gtttaggaac
    69781 tagatttttg ccagctacaa aggcacaacc aaaagagatg ttaccaatag ttgataagcc
    69841 aactctacaa tacataatag aagaagctgt ggcctcagga atagaggaaa tactaataat
    69901 aacaggtaga aataaaaaat ctattgagga ccactttgat aaatctgtag aattggaact
    69961 tgatttagag aaaaaaggta agaaagaatt gttggaggta gtacaaaaca tatctaacat
    70021 gattaacata cattatataa gacaaaaaga gccaaaaggg ttgggagatg caatttattg
    70081 tgcgaggcat tttataggtg atgagccgtt tgcagtaatg ttaggggatg atattgtaga
    70141 taatgatgta ccttgtctaa aacaactaac agatgcatat gaggaatata gaactacaat
    70201 attgggtgtt caaaaggtaa atcaagaaga tactaataaa tatgggataa tagaagcaaa
    70261 aaatatagaa ggtagagttt acaaggtaaa agacatggta gaaaagccag agtcaggaaa
    70321 agctccttca aatattgcta ttttaggtag atatataata actcctgaaa tctttgatat
    70381 tcttaaggat ttaccaccag gtaaaggtgg agaggtacaa cttacagatg ctttaaaaat
    70441 attatctaag aaagaagcta tgtatgcata caattttgaa ggcaagagat atgatgttgg
    70501 ggacaagcta ggatttttag aagctactgt tgactttgct ctaaagaaag aagaccttaa
    70561 ggaagatttt ataaaatatt tgaaacatgt atgtagtgaa tttgataaaa ataacaatgt
    70621 gttatacaat aatacagatg aaaaaataga tttaattgaa gtcaatgaag ctagagaact
    70681 tgaaattcaa aaataattaa tgaaaggtga tgtatggtgg caaaatcaag tcagaaaaga
    70741 attaatagaa taatagctgt tgggattgtg ggaatcctcg taataacgag tataacagct
    70801 gttttgagta tgattgcaac tatgtaataa gataaatgac agcagttgat atacataaat
    70861 ataaaaacca tatataactt gatgtagtca aaaacttaca atattttagt tatatatggt
    70921 ttttagaatt tataaatgta aaaaaaaatt tttataatag ttgcatattt taaaggaaaa
    70981 tattattata agatataaat atagtgataa atttataaaa acgtgctaag attggacttg
    71041 aaatacagta atatagtagg taataatgtt ttaaatttta tttattgacc ataatatata
    71101 aataatgata ttattagact taactgttta aataaaactt ttctttttca tgccttcaat
    71161 ttccaaaata agtatgtata taaatgaata tatagatgag gaggattttt ttgattttag
    71221 atgataaagt attagaagga ttaggtagta agaccttagt tgagctacga gagatagcta
    71281 aagaattaaa aataaaatca attactacat ataagaaaaa tgagttaatt gagattataa
    71341 attccaaaag taaaaatgag gaaaaagtaa acgaagagac taaagaaaaa aatgaagata
    71401 ttaaagaaga cataaagaat agaaaagaaa atatagagcg tgaaagtata gaaaaagaag
    71461 ttgaatataa gagccctacc aaatcataca acaagaaaga aatagatacc caaaatattt
    71521 cccaaaacca agaacataga aatcaaagaa atgactataa taagacaacg agtgtaaatg
    71581 atagtaataa gtcatcaaat atatctaata gaatggtcag aaataataat aaaaactatt
    71641 atatgcctaa acaagttgat gaatctaaga tagtagatga atttaacaca tcaaaagagg
    71701 atgaagtagt aggtgtactt gaaattctgc cagatggatt tggtttttta agaggttcaa
    71761 attatttgtc tactgaagga gatgtttacg tttcaccatc tcaaataaga agatttaata
    71821 tgaaaacagg agacaagatt aagggaataa ctagacatcc aaaaagtgga gagaaattta
    71881 gagcactttt atatgttcaa aaaataaatg atgaaaaccc tgacacagct atacaaagaa
    71941 atgcgtttga gacattaaca ccaatatttc cagaagaacg acttacactg gaaactaata
    72001 gaaatgaaat agctacaaga attatagatt taatttcacc tataggaaaa ggacaaagag
    72061 gattgatagt agcacctcca aaagctggga aaacagttct tttaaaaagc gttgcaaata
    72121 gcatagctaa aaatcatcca aatgttgaac ttatagtact ccttatagat gaaagaccag
    72181 aggaagttac tgatatgaaa gaatctattg aaggagacgt tatatattct acttttgacc
    72241 aagtatcaag tcatcatgtc aaggttgcag agatggtgtt aaatagagct caaagattag
    72301 ttgaacatgg taaagatgta gtaattttac tagatagtat aactagattg gcaagggcat
    72361 ataatcttac aatatctcct acaggtagaa ctttatctgg aggtattgac cctggtgcac
    72421 ttcatggacc taaaaagttc tttggtgcag caaggaatat tagacagggt ggttctttga
    72481 caatattagg aacagcattg gttgaaactg gttctaggat ggatgatgta atatttgagg
    72541 aatttaaagg tacaggaaat atggagctac atttagatag aaaattagca gaaaaaagaa
    72601 tattccctgc aatagatatt tacaagtctg gtacaagaag agatgattta ttgcttgatg
    72661 atgaagaaaa gactgctctt tggagattaa gaagagaaat gagtaataat tcagtaatgg
    72721 aaattacaga taaagtcatt gaacttataa agagaactaa ggacaataaa gagtttgtaa
    72781 aatcaataaa gaatttgtaa aatcaataat acaaatgcaa atatggatta tataaactaa
    72841 tagaaaaatc acttgaatgg cataataagt tgtgctataa taatgtagtt gataatgtaa
    72901 aaagaaagtt aatataatta atttttttaa ataaagagaa gaggtgacta taatgcaaaa
    72961 ggaaatacaa ccaaaataca atccagttga agtgcgttgt gcttgtggga acacatttgt
    73021 tgctggttca actaaggacg aaataaaagt tgaaatatgt tcagagtgcc atccattcta
    73081 tacaggaaaa caaaagaaca tagaaaaagg tggaagaatc gacaaattca agaaaagatt
    73141 caaaatggac taatttttat aaaagtggag ttggaggaag ccaactccta ttttattaca
    73201 aaaaaacatt catattctac tttaaattgt ctttttgtat tataataata aatatgtaaa
    73261 attgagtatt ctaaatacaa taaaatgagg tgagtggatg gcaaaacaag ctgtaggtgg
    73321 gcaagctgtt attgaaggtg taatgatgca gtcaaaagac aaaagagcgg tagctgttag
    73381 aaaaagtgac ggagaaatag cactaaaaga agatagaata aaaagttggg taagagataa
    73441 gaatatagat aagatacctt ttgttagagg ttcttttgtt atgatagata caatgattca
    73501 aggcataaaa agcttaaact tttcttcaga gttttttatg gaagaagctg aagaggataa
    73561 atttgattta tttataaaga aaatttttaa agataaggca aatgatataa ttataatatt
    73621 ttcattggtc atagcaatgt tactttctgc tggattattc atttttatac ctacattggt
    73681 aggaggagct ttttctaaag taatgcctaa tgattttatg cttaatttaa tagaaggaat
    73741 cataaggata gctatattat ttgcatatat agtattgatt tcacgaagta aggatataga
    73801 aagagtcttt caatatcatg gagcagaaca taaatctata tattgctatg aaaatgattt
    73861 agaacttaca gtagagaatg ctagaaaatt taaaagatta catcctagat gtggtacaaa
    73921 tttcctattt atagttatgg ctgtatcaat aatattgttt gcattttttg gttggccaaa
    73981 tccaattctt agaatattta tgagaattat atgtgtgcct atagtagcag gtttatctta
    74041 tgaggtaatt agagttcttg ggaaatatga taatggattt actaaaatta ttgcatatcc
    74101 aggaatgatg ttgcaatatt ttacaacaaa agagcctgat gatgagcaat tagaagttgc
    74161 tttggaagca ttgaaagcag tagtagatta gagaaaagga tatttgttat gacgataaaa
    74221 gatataatta ttaaatattc agataaacta aaagatataa gtgatactcc aagattagat
    74281 acagagttat tgttacaaaa aacattgggt gttgatagat tatatattca cttaaatctg
    74341 aataaagagc taactgaaga acaaaaaaca aaatttatgg gttttgcaga agaaagatta
    74401 aatggaagac caatagcata tatagttgaa aatagggagt ttatgggatt agattttttt
    74461 gttaaggaag gcgtattgat tccaagacca gatacagaaa ctttagtaga agaaataatt
    74521 gaaatatgta gagaaaaaaa agatgtaagt atattagata taggaactgg ttctggagct
    74581 attacaataa gtttagcaaa gtacattgaa aattctaaaa tcatgtcttt tgatatatca
    74641 gagacagcat tagaaatagc taaaaaaaat gctattataa atgaagttgg tgaaaaaata
    74701 aagtatatta attctgattt atttacagca ataagtgata gtaacataaa attcgatatc
    74761 atagtttcaa atccaccata tattaaaaaa caagatatag aaactctgca caaacaagtt
    74821 aaagattatg aaccatataa tgctcttgaa ggtggagaag atggattaga cttttataga
    74881 agaataacag aacaaggtaa gaaatattta aataagtgtg gaatattagc atatgaagtt
    74941 ggtcataatc aagctgaaga tgtaataaat attatgaaaa gtaatggata taaaaaaata
    75001 tacacaaaga aggatattca aggcattgat agagttgtta taggttacaa tatatgatat
    75061 aatcaacaat tgatatgtta catttaatcg aggtgaaagt atgttaaaaa aattagaggt
    75121 attggaagat acatataaag acttaagtga aaaaataggt gatccggatg taataaatga
    75181 tcaaaaagtt tggcagaaat atataaaaga acatgctgac ttggaaccta ttgttatgaa
    75241 atacagagag tataaaagcg ttttagatag tataaaagaa tcaaaagaaa ttttacaaga
    75301 agagtcagat gaagaattaa gagaattagc taaaatggag ttagctgaga tggaagaaaa
    75361 agtagcacct ctggaggaag aaataaaaat actattattg cctaaagacc ctaatgatga
    75421 taagaacgtt atagttgaaa ttagaggtgg agctggagga gatgaagcag ctctatttgc
    75481 tggtgattta tttagaatgt actcaaggta tgctgaaaga agaagatgga aaatagaatt
    75541 attgagtgca agtgatactg gagttggtgg atataaagaa gtatctttta tgataaaagg
    75601 taaaggtgct tattcaagat taaagtatga atctggagtt catagagttc aaagaatacc
    75661 atcaactgaa tctggtggaa gaatacacac atcaacttct actgttgcag tattaccaga
    75721 agttgaagat gtagaggttg aaataaatcc aaatgattta agaatagatg ttttccgttc
    75781 ttcaggtaat ggaggacaaa gtgtcaatac tactgactct gctgttaggg taacacatat
    75841 acctacaggt gaagtagtat cttgtcaaga tggaaaatca caattaaaaa ataaagagca
    75901 agctttaaaa atattaaaag ctagacttta cgataaagca ttggcagaac aacacaaaga
    75961 tatagcagca gaaagaaaaa gccaagtcgg aacaggagac aggtcagaaa gaataagaac
    76021 atataatttc cctcaaggta gaataagtga ccatagaata aatttaactt tatataagtt
    76081 agatgcattc ttagatggag atatagatga aatgatagat gctttaatta ctgttgacca
    76141 aacagaaaaa atgactgcaa tataagtaat gttttaaaga agctgtttct tcctagaaat
    76201 tattttctat tttgaaacag ctattttgca ttttaggtca tgttatcata ttctattgta
    76261 tataattgtt atatacaagt ataggagggt tatatgatac taaaagtaac aataattggg
    76321 cttttggcag gtgttatagg gacaggatta ggaggagtaa tatcagccat ttttaaaaga
    76381 gaggtcgata aatatctaag tttttttatg ggattatctg gtggaataat gttggctgta
    76441 gtggtgtttg atttaatgaa agaatccatg gacaaaatgg ggataatcaa tacagttata
    76501 tttacgtttg ttggagctct cataactatg tacataaaaa caaaattaga tgtgtctgga
    76561 aatatggcat ctggatatct tatattcata agtatattac tacataattt accagaagga
    76621 ctagccattg gttcttcttt tatgtcaact gaaagtttag gtattacact tgctatagtt
    76681 ataggtctac ataatatacc agaagggtta gctatggcat taggcttagt ttgcaataaa
    76741 atgaaactaa gtaaagttat tttgtttact gtaatagctg gattacctat gggtctagga
    76801 agttttttag gtgtttattt tggaggagta tttacttctc ttataggagt atttcttgct
    76861 acagcaggag gtactatgat gtatgttgta ttggaagaaa tttttccaca ttcaaaaagt
    76921 gtgtattgta taattggttt cttgttaggt acaatgattg taaattatat ataaaattaa
    76981 aatgaaagca ggcatacaaa agatgaaaac tattataagt aatattgata taaataatat
    77041 tgattatgag gaaataaaaa tacaagcaaa acttcttaga gaaggtaaaa cagttatttt
    77101 tccaactgag acagtatatg ggttgggtgc aaatgcatta gatgaaaatg cagtaaagaa
    77161 gatttatgaa gctaaaggaa gacctagtga caatccattg atagtccata tatatgaaaa
    77221 agaagaagta tatgacttag ccaaagatat aagtgataaa gctaaacttg ttatagaaaa
    77281 gctttggcca ggacctataa caattatatt aaataaaaaa gatataattc catacaaaac
    77341 tagtggaggg cttgatacag ttgcaataag gatgccttct aatgttattg ccagggcaat
    77401 aataaaagaa gctggtatac ctattgcagc tccttctgca aatatctcag gtcgcccatc
    77461 tccaacaaaa gcaaaacatg tatatgaaga gatgaatgga agagtggatg gaatagtttt
    77521 aggaggagac agtaattttg gattggagtc aactgtattg gatttaacag aagagactcc
    77581 tatgatactg agaccaggaa gtataactaa agaagtttta gaggaacttc ttggagaagt
    77641 aaagcttgac ccatcattaa gtaaaaaaga agataatcaa aaagctaaag ctcctggaat
    77701 gaaatataaa cattattctc caaatgcaga tgtatatata atatctggaa aaagagaaaa
    77761 tgtagcaaca aagattaatg atatgataaa atctaataga gacaaaggtc taaagacagg
    77821 tgtaatgtgt attagtagaa accgagagtt ttatgatgga gaagtaatcg atttaggaaa
    77881 ctctcttgaa gaagttgcaa gtaatctgtt tgatgctctt atagaaatgg acaaaaaggg
    77941 tgttgatgtc atatattcag aagagttccc taagactgga gttggacaag caattatgaa
    78001 tagactactg aagtcagcag gatacaaagt gataaaggct taagagggag ttgtaaaata
    78061 tgaatatact tattgtgtgt acaggtaata cttgtagaag tcctatggct gaggctatac
    78121 taaggaaagc aattaaagaa tctggaagaa gtattgaaga atattctata tcatcagcag
    78181 gtatatctac agcaaatgga atgggtgctt ctgaaaattc tatagaagta ctaaaagaaa
    78241 tcggtataga cttatcaaat catagaagta aagtaattac taaaaaatta attgatgaat
    78301 cagatataat actaacaatg actaaatctc ataaagaaat tttagtacaa gcagttccaa
    78361 aatgcaaaga aaaagtgtat acttttaaag ggtttgcaaa taaaaatgag gaagatatat
    78421 ctgacccatt tggtggaaat ttggatattt atagaagcac tatgagagaa ataatgtatt
    78481 ctgtcaacga aatagtaaaa aaaatatgaa acggaggata aacaaatgaa gataggatta
    78541 ggctgtgacc atggaggata taatttaaaa aaagaaataa taagctattt agaaagtaaa
    78601 ggcatagaat gtgtagatta tggaactaat aatgctacag attctgttga ttatcctgtt
    78661 tatggagaaa tagttgcaaa ttcagttata aataaagaag tagattatgg aatattatgt
    78721 tgtggtacag ggataggaat atctttagct gctaataagg ttcctgggat aagatgtgca
    78781 gttgtatcag atgtattttc tgcaaaaatg tcaaaagcac ataatgatgc aaatatgtta
    78841 tcattaggcg aaagagtttt aggaaaaggt ctagcacttg agatagttga agcatggatt
    78901 aatacggatt ttgaaggtga tagacatgct agaagagtaa acatgattaa atcaatagaa
    78961 gaaaaacata ataaataata aaaattacag ttttatcagg aggaattatc aaaatgagca
    79021 aagtggtaga gacaaaccat ccattaatac aacataaatt aactttaatg agagataaaa
    79081 atactggttc taaagacttt agagagcttt taactgaaat tgcaatgtta atggggtatg
    79141 aaataacaaa agatatacca ttaaaagatg tagaaataga aactcctatt caaaaaacat
    79201 catctaaagt agttgcaggt aaaaaattag caataattcc aattttaaga gcaggtcttg
    79261 gaatggtaga tggattagtg agcttaatgc cagcagcaaa agttggacat gtaggactat
    79321 atagagaccc agaaacatta aaaccagttg aatattattg taagcttcct caagacatag
    79381 gagaaagaga tataatagta gttgacccta tgcttgcaac agggggttct gctgtagcag
    79441 ctatagattt acttaagtct aaaggagcta aaagtataaa attagctaat ttagtagctg
    79501 caccagaagg tatagcagaa gtgcaaaaat atcatgatga tgttgatata tatgttgcat
    79561 cagttgacga aagattaaat gaacatggat atataattcc tggtcttggt gatgctggag
    79621 atagattgtt tggaactaaa taaaaataaa taactataaa tatttattag ggactttctt
    79681 atatagtgta tataatttta tgtaagaagg ttctttttta tatatttttt ctcaaaatag
    79741 gaattattga attgataaat ttctcgaatt gagaataaaa agtttttacc aattaaaaaa
    79801 gattgcttat tttacaaggt tttatagaat agtagtctat tttaatgtgg catgttagtt
    79861 gcttacgatt aagattatag gtattaagtg tattagttaa attgagaatt gaatattaat
    79921 aatattattg ggagtggtaa tacatgaaga attgtatagt agtaagaggg gctggagatt
    79981 tagcaacagc agttattcat aaattacaca acagtggttt taaagtagtt gcgttagaaa
    80041 tagaagagcc attagctata agaaggcaaa tttctttttg tgaggctgtt tatgaaaaaa
    80101 aagtaactgt tgaaggtgta gaatgtatat tttgcaatac tgaaaaagaa atagaagaag
    80161 ttttatctca acataaagta gccttgatta tagacccaaa aggagaaaaa ttatctaaat
    80221 taaatccaat cgttttggtt gatggaatac tcgctaagaa aaacttaggt acaactaaag
    80281 atatggcacc acttacaata gctttaggtc ctggattttg tgcaggagaa gatgtagatg
    80341 ttgttataga gacaatgaga ggtcataatc taggaagaat aataactaat ggtagagcag
    80401 aaaaaaatac aggagtacca ggagcaataa aaggtttttc taaagagaga gttatgcatt
    80461 cacagtatga tggcataatg gaaaatgtat gtaagatagg tgatatagtt gaaaagggag
    80521 acctactttg ctatattaat aattatgaaa aaaaacatga agttagagca acaatatctg
    80581 gagtccttag aggtttatta aaggatggag caaaggtaaa caaaaattta aaaatactag
    80641 acatagaccc tagaaaagaa gaagttaaga atgcttttac aatatcagat aaagctagat
    80701 gtatagctgg aggggtttta gaagccatac taaacaatgg gatttatccc caaaattaat
    80761 ttatattaga taaagctatt aatattttat taagatgata taataaattt aatagattaa
    80821 agagagcata tgacgggtat ttaaaattta aatggtaaaa atgctctttt tttattggac
    80881 attcaattaa catgtgtata taatataaaa ataagtattt atttaggttg aaatctaata
    80941 aatatataat accagtacat aagcaaagag gagaaaagta aattatgaga ccatcatggg
    81001 atgaatactt catggaaatt gcagaagtag tgaaaaagcg ttcaacttgt attagaagac
    81061 aagtaggagc tgtaattgta agagacaaac aaatattaac aactggatat aatggttctc
    81121 cgagaaatct tgaacactgt gaaaatattg gatgtaagag gcaggaatta aatattccct
    81181 caggagaaag gcatgaatta tgcagagcat tacatgctga acaaaatgcg ataatacaag
    81241 ctgctcataa tggtattagt gtagatgggg caactttgta tgttactaca agaccttgtg
    81301 ttttgtgtgc aaaaatgtgc ataaatgcag gtatattgaa aattgtttat gaaggtgatt
    81361 atccagatga tatgtctaca gaattattaa aagaagcagg aatagaactc ataaaatttt
    81421 agtggataaa atttatatga caataaatgt caaattatga cactgaaata caatggtatc
    81481 aaatgattaa aagaaagtat ttgaaaaatc atcagattag tagtaagata tattttataa
    81541 ttaaataaat ttatgagata gattaattac atgttattta ttttataata atgagggata
    81601 ctatttatga gtaaaagaaa tacacataca gaaattgcaa agttgtttag tttaataagc
    81661 caaataggat tgatgattgt aatatcaata ttaggatgta ctttcttagg aaaattttta
    81721 gatagtaaat tgaatactac tccagttctg acaataatat ttttattgtt gggtgttgga
    81781 ggtgctttta gtggtgttta taaaactcta atagtatata cacgaaggaa gtgatatatt
    81841 ggatataaaa ttacttaacc aggtaaggtc agtaacaaaa ggtatagtaa tatttgatgt
    81901 agttttaatt gtgctaatgt tgataacatc aacattaagt aaaccagtat tattaggact
    81961 tatatttggc tctataatag ctatgttaaa ttttagatta ttggcgatat cactggaaaa
    82021 atcagtgaca tttcctgctg ggaaggcaca agcttatgca tcagcacaat atgctgttag
    82081 attatttata atggcagttg ttctaattgc atcagctaca gtaccacatt taaatataat
    82141 cggaacagca ttgggattat tgagcacaaa gtttgtcata ttatcaaaaa actttataaa
    82201 taaattaaag aggaaggagg cataagtatg aatcaagcgc attattatct cataggaaac
    82261 tttaagatta gtgaaacaat actagtaagt tggggaataa tggccttttt agcgatagca
    82321 tcttattttt tgactagaaa cttaaaaaaa gttccaacta gtaaagttca aatattctta
    82381 gaatttgcag taggtggtct tgctaaaatg gttacagatg ttatgggtaa aaatacagta
    82441 aaaagaatgc catatatgat accatacata ggaagcttgt ttttattttt cgcatgttca
    82501 aatttaagcc cattacttgg attaaggtca ccaacaaccg acttagatac aactgctgca
    82561 tgggcaatga ttacattttt tatgatttat tttgcaggag ttaaatttaa ggggttatca
    82621 tactttaaag aattggctca accacttgcc ataatgttac caatcaatat tataggtgag
    82681 ttagctagac caatatcatt aagtttccgt ccatttggga atattttagg tggaacaata
    82741 ataatgggtc tgttttatca acttttaggt tttatttcta gtttaatacc aggattatca
    82801 ataccaattg gagaaatatt aataccagtc ccattacact tgtactttga cttatttgca
    82861 ggattgttac aagcatatat atttataatg ttaacaatgg tatttatatc aagtgcagga
    82921 gagttagatt aagaaatcaa cttatattaa gataaaaata aaataattta aaaaattaaa
    82981 gttttaagga ggaaataatt atggaaacag caatagtagc ggcagcatca gcaataggag
    83041 cagggatagc agttgcaaca ggtataggtg caggtatagg tcaaggtata gcagcagcta
    83101 aagctgcaga agcagtagga aaccagccag aagctaaagg tgatataaca tcaactttat
    83161 tactaggagt tgctatagca gagtcttcag caatatatgg tttggttata tcaataatac
    83221 ttctattcgt aaatccattc tttaaatact taggaatgta gatttagtcc ttaaatattt
    83281 tgagataggt ttataggtaa atcatctcaa acttataatc atgagatgaa ctttatcatc
    83341 tcgtgattat aagtagttat gatttatgga aaggaggata attatggaaa aagcactagt
    83401 aggtataacg tgggaatttg tgttccaaat tgttaatact tttataatat ttttacttct
    83461 gagaaagctt ttattcaagc cagtattaaa tataattgaa tcaagagaaa atgatattaa
    83521 aagtgatttg gtagaaggcg aaaaagcaaa aaatgaaggt ttagctctga aaaaagaata
    83581 tgagtcaaaa atcaattttg ccaaagatga aggtcaagaa atcataaaac aagcaactat
    83641 aagagcagag cagaaatcag atgatatagt aaatactgca aagaaagatg ctcttgatat
    83701 aaaagaaaaa gcaaacaaag atatagaaca agaaagacaa aaggttataa atgaaatcaa
    83761 aaatgatata tctaacatag cactacttgc agcatctaaa gttattgaaa aagatttaga
    83821 taaatctaaa catgaagaat tgatagaaaa ctttataaaa gaggtagggg aagctaaatg
    83881 ataaatgtaa tagcaaatag atatgccgaa gccctatttc aattaggaga agaagaaaat
    83941 tcaactgatg tcttattcaa agaactagaa aaagtagtag atatgatgac taaagttagt
    84001 aaagatttct acaaagtttt aaaatcacca ttagtttcaa aatcagaaaa gaaaaacttg
    84061 gtagaaatta tttttagtaa agaagtaagt tctaacataa aaaacttttt aaaagtttta
    84121 gtagataaag atagaatatc atatttagaa gatatcgaat tagcttataa agaattatta
    84181 aataagaaaa acaatgtaat tgatggagta gcaataagtg ctattccaat gagtgaaaca
    84241 gatattaaag aacttgaagt caaactttca aataaataca acaaaaatgt tactatagaa
    84301 aatgttgttg acaagactat actaggggga gttctagtta gaattggaaa tgaacaaata
    84361 gatggcactg tgaaaactcg tcttgataag atgaaagaaa agctatctga agtaatatct
    84421 taagaatggc ggtgaaccca tgaacttaaa acctgaagaa ataagttcta taattaaaca
    84481 gcaaataaaa aattatgaga ataaagttga gttgacagat acaggtagtg ttttaacagt
    84541 tggggatggt atagcgagtg tatatggatt agaaaaagct atgtctggtg agttgctaga
    84601 gttcccaggt gaaatatatg gtatggcact taacttggaa gaagaagtag ttggagctgt
    84661 tatccttgga gatgacagtg aaataaaaga aggcgatata gttaagagaa ctggaagaat
    84721 agttgaagtt ccagttggag aagctttaat aggtagagtt gtaaactcac taggtcagcc
    84781 aatagatgga aaaggaccta tagcgtacac aaaaactaga ccagttgaat ctgaggcacc
    84841 tggtataata gatagaaggt ctgtttatga accattacaa acaggaatca aatcaataga
    84901 ttcaatgata ccaattggta gaggtcaaag agagttaata ataggtgaca gacaaactgg
    84961 taaaacatct atagttattg acactatact aaatcaaaaa ggaaaagatg ttatctgtat
    85021 atatgtagca attggacaaa aacgttctac tatagctcaa ttagttagct ctttagaaaa
    85081 aggtggagct ttggattata caatagtagt ttcagcaaca gcatctgaat cagcaccact
    85141 tcaatatata gcaccatatg ctggagcagc tatgggtgaa gaatttatgt acaatgggaa
    85201 acatgtactt atagtgtatg atgatttaag taaacaagct gttgcatata gagaaatgtc
    85261 attgttactt agaagaccac caggacgtga ggcatatcct ggagatgtat tctatttaca
    85321 ttcaagatta cttgaaagag cagctaagtt atctgatgaa ttgggtggag gttcaatgac
    85381 tgccttgcct ataatagaaa ctcaagctgg tgacgtttct gcatatatac caacaaatgt
    85441 tatatctata acagatggtc aaatatactt acaaccagag ttattctatt caggtgtaag
    85501 accagcagtt gaccctggta tatcagtatc aagggttggt ggttctgcgc aaattaaagc
    85561 aatgaaaaaa gttgcaggaa cattaaaact tgcatattca caatatagag aacttgcagc
    85621 attctcacag tttggttctg acttagatga agatactaaa aagagacttg ctcaaggtga
    85681 aagaatcgtt gagatattaa aacaaggtga gcatcaacca ataaaagttg aaaatcaagt
    85741 tatgataata tatgctgtta taaataatca tttagaggat attccgatag ataatatagc
    85801 tagatttgaa tcagaattgt atgcatttgt agataataat tatccggaaa tatcaagaaa
    85861 aatattaggt ggagaagact tcactcatga tttaacagat gcaataaatg aatttaaaga
    85921 aaagtttgtt gttgaagtat aatccttttt aaaaaaggag gtaaattaat tggcaggagc
    85981 tggaatcaaa gcaatcaaaa cacgtatggg aactgttgaa agtacaaagc aaataactaa
    86041 agcaatggaa ttagttgcat cttctaagct tagaaaagca aaagaaaagg ctgaagattc
    86101 aagagaatat tttaatactt tatacaatac agtaaaagat attgcccaaa caacacgtgg
    86161 aattagaaat gaatttttaa aagaaagaga agtaaaaaat aaatgctatg tagtagtagc
    86221 tggagacaga ggacttgcag gtggatacaa ttctaatgta ttaaaggaat ctgtctctca
    86281 tatgaaaggt aaaaaggaaa cagtgattac tgttggaaag aaagcacatg aatttttttc
    86341 taaaagaaac tacaatatag caaaagatat aaatagtgtt gaagcttgtg gatatgaaga
    86401 tgcgacagaa atttcacaaa ctattattga cctgtataaa aaaggtgaag tagatgaagt
    86461 ttatattgct tatacaaaat ttaaatctgc tttatcacaa gaagttgaaa ttttgaaatt
    86521 gcttccatta gtctttgaaa aagatacaga cgaacaagga atggcagtaa aagaagaaaa
    86581 acaaaataga tcaaaagtac aatatatacc atcagcagaa gcagttttaa gttatattgt
    86641 acctcagttt atatcaggta cagtatatgg tggaattttg gaatcttttg catcagagca
    86701 aggtgctaga agaactgcaa tggaatcagc cacagacaat gcgaatgaaa tgctatctaa
    86761 tttagagtta agttataaca gagcaagaca gtcagctgta actcaagaaa taacagagat
    86821 agttggtgga gttgaagctc taaaataggg aggttaggaa atggcaaatg taggtaaagt
    86881 agtacagatt gtaggagctg tactagatgt taagtttgat agtgaacaaa gcctacctaa
    86941 cttattaaat gcgttagtaa taaagttagg agataaagaa atagttgcag aagttgctca
    87001 acatataggt gatgatactg taagatgtat agctatgagt gcaacagacg gacttgtaag
    87061 aggtatggaa gttgtagata caggagggcc aataagtgtt cctgttggag atgaaacttt
    87121 aggtagaata ttcaatgttc ttggaaaacc agttgatggg aaaccagctc caaaatctgc
    87181 acctaaatta cctatacata gaccagcacc ggcgtatgat gaactagaaa ctacagcaga
    87241 gatactagaa actggtatta aggtagttga cttactagct ccatatctaa aaggtggaaa
    87301 aataggtcta tttggaggag caggagtagg aaagacagtt ctaatacaag agcttataaa
    87361 caatattgct aagcaacatg gtggtatttc tgtattttca ggagtaggag aaagaacaag
    87421 agaaggtaac gacctttatg gcgaaatgag tgagtctgga gttataaata aaacagctct
    87481 agtatttggt caaatgaatg aaccacctgg agcgagaatg agagttgctt taactggact
    87541 tacaatggca gaacatttta gagatgagca aggacaagac gttttacttt tcgttgataa
    87601 tatattccgt ttcacacaag ctggttcaga agtttcagca cttctaggac gtatgccatc
    87661 agctgttggt tatcagccaa cattagctac tgaaatgggt gcacttcaag agagaataac
    87721 atcaactaag aaaggttcaa taacatctgt tcaagcagta tatgtaccag cagatgactt
    87781 aactgaccca gcaccagcta caacattctc tcacttagat gcaaaaacag ttttatcaag
    87841 acaaatatca tcactgggta tatatccagc agttgaccct cttgaatcta catcaaggat
    87901 tcttgaccct agtatagtag gaaaagaaca ttatgaagta gctagaggtg tacaatcaat
    87961 acttcaaaga tataaagaac ttcaagatat aattgctata cttggtatgg atgaattatc
    88021 tgatgaagat aagttaatag ttgcacgtgc tagaaagata caaagattct tatctcaatc
    88081 attcacagtt gctgagcaat ttacaggaaa tccaggtcaa tatgttcctg ttaaagagac
    88141 tgtaagagga tttaaagaaa ttcttgaagg gaaacatgat gacttaccag agtcagcatt
    88201 cttatttgta ggaacaatag aagacgctgt tagaaaagcg aagggaagta tgtagtcatg
    88261 gccagtgaat tttcattgaa agttgtaact ccaagtaata ctttttatga tggagatgtt
    88321 gaaatgatag tagttagaac tacaaaagga gacaaagggg tgttgaaagg tcatatacca
    88381 tttgtttcag gtatacctgt aggaacttta aagataaaaa aagagggaag ttttaaagag
    88441 gcgaaaatag cagaaggatt cattaatgta actcaagata aaactgtcat attgacagaa
    88501 tctgctgagt ggatttaata aattaattta gagtataaaa agtcattttg accaaaaaaa
    88561 cttttgggta aaatggcttt tttgtgtttt ggtgttaata aaataacgat aaaattgtaa
    88621 gtttttagtg ataataaatt cacaaaatgt ttaaatttac tgccaaatta tgttgtaatt
    88681 aataagataa agaagattta gatagctttt atgtaagtga aaaagtcttc atataagaaa
    88741 tacatataaa atttttataa aatttaaaaa atttgcttag ttgtgtagta taataaataa
    88801 tataatagat ttgtatataa tattcattac aaataatttt aaaagttaca gttttattat
    88861 aatgattatt aaattaagat aagtaacata aactaaataa taatgtgtat tatcgatagt
    88921 aaatttataa attcattatt aattattgaa agaagtatta taaattttct ggaggaacaa
    88981 tgaatatatt tgatattggc gtagatataa ttgaaattga tagaataaga aaggctgtag
    89041 acaaaaataa taggtttttg gagaagatat ttacagatag agaaatagag tattttaatt
    89101 ctaagaattt taaagcagaa agtatagcag gtaatttcgc agccaaggaa gctataagta
    89161 aatctatagg tacaggaatt agagtgttta attttaaaga tatagaagtt ctcagagatg
    89221 aaatgggaaa accaatagta aaaacttata ataatctagc aaaaatgtgc atagattata
    89281 atgttttaga aataaaagtt tcaatatcac attcaaaaga ttatgctata gcaaatgcga
    89341 taaccataat taaggactaa ggagtgatta cattatgatg gataaaacag caaaagaaat
    89401 aatgacaaca gatgtaatag tagcaaaaca agatgatagt atagcagatg tagctaatat
    89461 gctaattgca gaaaaaatag gaggtctgcc agttgtagat tcagagaata gagttgttgg
    89521 aataatttct gaaacagata ttcttaaaaa agaaaaatat atagaagcac ctctctatat
    89581 aaatctatta caaggattga ttttcttaga tgacttaaaa aaagttgaaa aagatataaa
    89641 acaagtagct gcttataagg ttggagaact tatgtctaaa gatataataa aggttcatga
    89701 agatgataag tttgatgatg tagctaatgt aatgattaaa aaatcaataa acagagttcc
    89761 tgtagtagat gatgacaaca aacttaaagg tataatttgt agatatgata taataaaagc
    89821 tttatacaat gaataaactg aagtaggagg tgtgatgtga gaaaaaagtg gaccatagta
    89881 tgtattatgt tcttggcact ggttataatt gtaataggat gccaaaaacg acagtccaca
    89941 aaagaagaag tatacaaaga ttttcaaaag cagatttctg atatgaatta ttacagttgt
    90001 aaagctgaag tagaagtagt tggaaataaa agtccacaca attatgtttt aattcatact
    90061 tataaaaaaa ccgataacta taaattagaa gtaatttccc caaaacattt aaaagggaaa
    90121 agtatagaat atcaaggtga taaaatatta gttaaaaatc caaagattag tgatgtagtg
    90181 gagttaccta atacaggaaa aaataatcaa tatctttttg taggagactt tataaaaaac
    90241 tatcttcaaa atgaggagat gaaagtaaag ttatccaaag gacatttggt tttagagact
    90301 tttattcctg gggataataa atattttaac aagcaagtat tatatgtaaa tgctgacaca
    90361 aaaaatcctg aaaaaatgga agtgttagat aaagagggag tgccaagatt tacagtaaaa
    90421 tacaaagatt ttgaatacag aaactaaggg ggactaaaga catgcaaaaa ataacagtgc
    90481 ctacatgggc agagataaat ctagataact taagatttaa cttaaataat attaaaaatt
    90541 tattagaaga agatattaag atttgtggag taataaaagc tgatgcatat ggacatggtg
    90601 cagtagaagt tgcaaaattg ctagaaaaag aaaaagtaga ttacttagca gtagcaagaa
    90661 ctgctgaagg aattgaactt agacaaaatg gcataacact tcctattttg aacttgggat
    90721 atactccaga cgaagctttt gaagattcta taaaaaataa aataactatg acagtttatt
    90781 ctttagaaac agcacaaaag ataaatgaaa ttgcaaaatc tttaggagaa aaagcctgtg
    90841 ttcatgttaa aatagactca gggatgacta gaataggttt ccaacctaat gaggagtcag
    90901 tacaggaaat aatagaatta aataaattag aatatataga tttagaaggt atgtttactc
    90961 attttgctac agctgatgaa gtaagtaaag agtacactta taaacaagct aataattata
    91021 aatttatgtc tgataaatta gatgaggctg gtgtaaaaat agctataaaa catgtatcaa
    91081 acagtgcagc tattatggat tgccctgatt taagattaaa tatggtaaga gcaggaataa
    91141 tattatatgg tcattatcca tctgatgatg tatttaaaga tagattagaa ttaagaccag
    91201 ccatgaaatt aaaatcaaaa atcggacata taaaacaagt tgaaccaggt gtaggaataa
    91261 gttatggact aaaatacaca actacaggta aagaaacaat agctacagtt ccaataggat
    91321 acgcagatgg atttactaga atccaaaaaa atccaaaggt tcttattaag ggagaagtgt
    91381 ttgatgtagt tggtagaata tgtatggatc aaataatggt tagaattgac aaagatatag
    91441 acataaaagt tggagatgag gttatactat ttggagaagg cgaagttaca gctgagcgta
    91501 tagctaaaga cttaggaact ataaactatg aagtgttatg tatgatatca agaagagttg
    91561 accgtgttta tatggaaaat aatgagcttg tacaaataaa cagttatttg ctaaaataaa
    91621 accatgatat tataatctgg gaggcgattt tgtgtacgat aagagtaaag aaaaaataga
    91681 agttacttta cctgactctc tgatttctga agttgatagt atagttcaga tggagaacag
    91741 taacagagaa gattttgcaa aagctgcttt tcagttctat ataactcaga aaaagaaaat
    91801 tcatattaaa gaatctatga aaacgggtta tagagaaatg ggtcaaatta atctgtcact
    91861 agctgaatta ggtatgacag ttgaagaagt atcttctgat gataaaaatg aagggaagat
    91921 tgcccaaggt gagtactaat tgtgaaataa agcgtggtga tttatattac gctgatttaa
    91981 gtccagttgt tggttcagag cagggaggcg ttagacctgt tctgataata caaaatgatg
    92041 taggtaataa atatagtcct acagttatag tatcagctat aacatctcag attaataaag
    92101 ccaagttacc tactcatgta gagataagtt ccaatgaata tgggcttaat aaagactcag
    92161 taattctttt agagcaaatt agaactatcg ataaaaagag actgagggaa aaaattggtt
    92221 gtctagatga aaatatgatg ataaaagtag atgatggact tcaaataagt ttaggattgt
    92281 ttacttttta aagagggatt tgtcttttat tagacaatcc cttttttctt ttaaatatct
    92341 cttaaggttt aattttaatg tactatgtta ataaaattaa aaataacgca aaaaaatagc
    92401 ataaaagtga tataataata tggaattgat ataaaattac gaactcaagg gaggtaattg
    92461 catgagagac cataaaggat taaatgagat tgcgcgtatc ataagaagag atatagtttc
    92521 aatgatacat agagccaaat ctggacatcc aggagggtca ttatcagtag ttgaaatact
    92581 tactgcgtta tactttgatg aaatgaatgt ggattcttca aatcctaaaa tggaggatag
    92641 agatagattt gtattatcaa aaggacatgc tgcaccagca ttatatgcta cattggctga
    92701 aaaaggttat tttgataagg aagaactgaa tggattaagg aagataggaa ggatgttaca
    92761 aggtcatcca gacatgaaag gcacgccagg tgtggaaata tctactggtt ctttaggtca
    92821 aggtttttca gttgcatgtg gtatggctat ggcatcaaag ttggataatg ctccgtggaa
    92881 tgtgtatact ttgcttggtg atggtgaggt tcaagaagga attgtttggg aagctgctat
    92941 gagtgctgca cattataaac ttgataattt aattgctttt ttagataata atggacttca
    93001 aatagatgga gatatagaaa gcgtaatgag tttaggttca atagttgata aatttaaagc
    93061 ttttggttgg aatgtaattg agatagatgg acatgatttt gaccaaatat ttgcggctct
    93121 tgatatagct aaatcaacag ttaaaaaacc tacaatgata gttgcaaaaa ctataaaagg
    93181 caaaggaata tcttttatgg aaaatcaagc aggatggcat gggacagctc caagtgatga
    93241 ggagctagaa aaagccttgc ttgaattagg aggtgctgac aatgagtaaa atggcgacga
    93301 gagaagcata tggtaaagca ttagtaaaat tgggtcaaat aaatgacaat gttgtagtat
    93361 tagatgcaga tttatcaaag tctacaaaaa cacatgattt ttacaagtca tttccagata
    93421 gattttttaa tatgggtata gctgaacaaa atttaatagg cgctgcttgt ggattatcaa
    93481 ctgctggtaa aatacctttt gcaagcactt ttgctatgtt tgctacggga agggcttttg
    93541 agattataag aaattcagtt tgttatccaa agctaaatgt aaaaatatgt gctactcatg
    93601 caggtcttac tgttggagag gatggagcat ctcatgaaag tgtggaagat attgctataa
    93661 tgagagctat accaaatatg acagttcttg ttccagctga tggagttgaa acagaaaaaa
    93721 ttatttttga aatagctaag tataatggac ctgtttatgt tagattaggg agaagttcag
    93781 taccagtttt atttgatgaa gattataaat ttgaaatagg aaaaggtaca gtcttaagag
    93841 aaggaaagga tgtatcaatt atagcttgtg gaattatggt aaatgaagct ctattagcac
    93901 aagaaaagtt gcaagaagaa ggtataagtg ctagagttat aaacatgtcc tcgataaaac
    93961 ctatagacaa agatttaata ttagaatcag caaaagaaac gaatgctatt gtaactgtag
    94021 aagaacatag cataattggg ggattaggtt ctgctgtaag tgaggtagta ggagagtctt
    94081 gtccaaccat tgtaaagaaa gttggaatta aagatacatt tggagaatct ggtactccaa
    94141 atgaattgtt aaaaaaatat gaattaactt gtgatgacat aataaagact gttaaagaag
    94201 ctattattgc aaaaagaatg taagagatat aaattattca tgtttgttaa taatataata
    94261 tatttaaaag agaggtataa agtgttatat taatttatat ctctcttttt tttatatgat
    94321 atcaatatat caaaaatgta ataaaatatt caaattttta caaaaataga gaaaatattc
    94381 aaatatatat tgactattca gaaaaatatg actaatatat aatttataga gaaactaatt
    94441 ttatatataa tgactactta gataaaagaa tctaaaaaaa ttatgagagg aggagtaggg
    94501 tataaaatca gattataagt tttaagttat tatactaaaa agctataaaa cagttatcat
    94561 gtattttatg ttaaaggaaa ttgtaggtag tttatcgaat tgtttaatca gaagaaaaaa
    94621 tttcacatgg ggaggtaact gagatatgtc agaaaaagta aatgttaaaa cagtcatcag
    94681 tttcgcaggt gcgtatgtag caacagtaat cggttctgga tttgcaacag gacaggaaat
    94741 aatgcaattc ttctcttttt atggttttgc aggtattatt ggtgggataa tttcaatggt
    94801 attattctca tggatgggtg cttcagttat gtcaaaaggt aaagagcttc aacttaaaga
    94861 acctatcaaa atttatcgtg tttattgtgg taagtattta ggaatattct ttgaatggtt
    94921 tggaccttta ttcttatttg gtgttttcgt agtaatgatt tcaggagcag gagcaacttt
    94981 aactgaatac tatggattaa atccatttgt aggtagagta ggtatggcag tagtagcatt
    95041 attaactgtt tcactaggtc ttgataaatt atcaaagata ctaggtggaa tagggcctat
    95101 aataataatc tttacacttt tagttggtgg tataagttta gctaaaaaca taggtaatat
    95161 aggagaagct gcacaagttc ttagcactgt aaagatagct aaaccagttc caaatgccta
    95221 tctatctggt gtaatatata ctacttataa cacaattgtt gttatggcat tcttaactgg
    95281 attaggagca agtgctgcta acaaaaaaga agctatttgt ggtggtatat taggtggagt
    95341 agcacttatg gctgcagcta taatgatgca cttagctatt ctttctgata taggaaactt
    95401 atattcaaaa gcaataccat cattattctt agcagataag atttcaccag taattggagt
    95461 tttattctca ataattctta tattaggtat atatacaaca gctgtacctt tattatggtc
    95521 agtaactaat agatttgtag atgacaaaca tcctaagttt aaattaataa ctttaatagc
    95581 agctatatta ggtcttatag gtggcttcct accatttgat aagctggttg gtattttata
    95641 tccatatact ggatatatgg gcgtcataat actggtttgt gttctttata gacagattac
    95701 aaaaacatct ggatatcaag aaggaatcag tgacaaataa gtatttttta atattgtaaa
    95761 ctcaagagct atatgaaaac aatgaaaatg ttttcatata gctcttttta ttttttagct
    95821 cgtttattat gaaatagaaa aaacatgttt tttagttaca ttacatatat ttataattat
    95881 gtataaatat atactaatca ataatttaga gtctaagata agtttataaa tcatatattt
    95941 agaatatatg ggaggagtga taagattgaa tgtaaagttt aatattaaag gtataattta
    96001 tggagttata gctcttgtat tcgttatagg gggatttata ttcataccaa gtatgtttgt
    96061 agagaaatct aaacccatag attatacagt attacaaaga aattcaatac cagaaaagat
    96121 attagatgtg atggataagt atacaaatga agaaagagca ttagcggcta aaatcgataa
    96181 caaaatatac attatagtga ctagaggaaa taataaatat ggtatagaaa tggacaaaat
    96241 agaaagtgtg gttgaggaag gaaaggatgt tttgagagtc aagataaagt ataaagataa
    96301 ggaagattct tttccataca tagttgttga aacaaatatg agtgaacttc cagatagaat
    96361 agaattaaat gcgacgaaat aagaaggtat tttataatta aataactgca tattgtacaa
    96421 taaagtcatg gtttttatta atgtaagtaa attgtgtatg gttcttattg ttgtaaaagc
    96481 catgacttaa aatgcttata aagtaagata ttaacagtag tatttattac tacattaaat
    96541 ggggtctgga aatacttaat attctaacta tttggagtga taattctagt ttcagtaact
    96601 atgtagtcta attgtgcatc atgagtttct tttggaatag aatcgaatat ctggaagtca
    96661 aatgctatgc cgatagttat agcatctttt cttaagtttt ctaaaaatct atcataaaat
    96721 cctccaccat atcctaatct atagcaatgt atatcatatg ctacagctgg tacaattaca
    96781 acatctaagg ccttaatatc aattgtaggt gatttttcgt taggttctct tattccatat
    96841 gctccaattc tcaatccctc ttttaaatct gttatttgag tagggattaa tgtatgattt
    96901 tcttttattg tgatgggact tgatactatt ttctttagag acaaaagttt tttgattaaa
    96961 ttatctgttt gaacttcatt gttaaaattt agataaagca ttatattagt tgcatttctt
    97021 atatcttcca tttgaagtaa tttttcagtt attaaatttg aattactaag tacaaagttt
    97081 gcattcttat tttttcttga ttcaataact ttttttctaa attctttttt cataaaataa
    97141 ttcctccctt aatatgttat aatgtgtaca gcaattgtat ttaatataaa ttttagtata
    97201 atttattgta ataaagcaat atatttgggt aaatgtaata aaaataacag ctagtcatat
    97261 aataataata gtttatgtaa aaggagatgt atcttgtgat ttcaaagaag aaagctattt
    97321 ttttaggggt gattttagta attataacag ctatggttac atcagcattt cagttgacat
    97381 tggggaataa agttgtaata tcgaaagaac tctatgaaga ctataaaaaa tatgataagt
    97441 tgttaggact agaatctatt ataaaacaag atttttataa aaaagtatca gatactgatt
    97501 tggtaaatgg agcagcaaaa ggtctatttc taggaacaaa tgataagtat tctggttatt
    97561 atactaaaga tgaaatggag aatctaatta atgatagtga aggttcttat gttggtgtag
    97621 gtatgtatat aggggcatca aaagatggtg gcttagtagt agttcccatg aaagattctc
    97681 cagcagagaa agccggtgta aaatcagggg ataaattagt aaaagtcaat ggaaaatctg
    97741 tctcatataa aaattcagat gaagctgttc gtatgatgaa gggtaaaaaa ggtaaaacag
    97801 ttgaactgac tatattaaga gaagataaac aattgaactt taaagtaaaa acagaccaga
    97861 taattgagaa gagtatagaa agtaaagtaa ttgataatga tttaggatat atagaaatta
    97921 cccaatttat atcaagtaca tatactgact ttgataaagc tttaaaagaa ttaaaagcaa
    97981 aaaatatcaa gggattagta atagacctta gaaataatcc tggtggaatg ctagatatat
    98041 gtaaagaagt tgctgatgag ttaattggtg aaggaactat agtatataca aaagataata
    98101 agggaaatac tgagtacttg aaatctgata aagaaaaatt gggacttcca atagtagtgt
    98161 taactaatgg agaaagtgct tctgctgctg aaatattaac agctgcaatc gttgataata
    98221 aagaaggtat ttctgtaggg actacaactt ttggtaaagg gttggtacaa tcagttgtaa
    98281 gattgaagga tggaacagga tacaagctaa ctactgctca atattttaca cctaatgggg
    98341 attatatcaa tgaaaaaggt ataaagccta ctattgaaga gaaagatgaa aataaacagt
    98401 tagatgtagc aactaagtgg cttagagaac agattgataa ataggattta atttttataa
    98461 acttatacta taaaataaag aacattataa aagagatgtc tcaaaatgaa ctttttagtt
    98521 catgagggag tctggaaata tttatgtgta ttctttaaat aaaaatttat tttttaagtg
    98581 ataattaaat ttttacagac agggctatgg ttttaaaaat catagcctta ttttgatatt
    98641 ttttaaaaac atattgcaac ccagttgttc caaatattat atcatttact tttaaaatat
    98701 cgcaaactgc atattatata ttcattataa tataatttat ctaactagaa cctcagttta
    98761 ctaattaaat tagatgcata gaataacttt tttaaacatt gaaaataaat taaactctaa
    98821 acacatttaa atataaattt atatatcatt ttgacttggt taaacaattt catacttatc
    98881 gatataaaaa taggttataa tataaatgtt cattatctta taacctattt tatataattt
    98941 attcctaaaa catgtgaatg ttttttatga tagtttgaag aaattataaa atcactttat
    99001 aattgaatat tatatataaa ttaatttaaa tatagaaagg aaaatatatg tacaacaata
    99061 tcaaaaatat tttaaaagaa aatcatataa cgtttgaaga atatgaccat gaacctattt
    99121 tatcatttga aaaagctaaa gaaataagag aaaaattaca atatactgga gtagaaagta
    99181 aaagtttatt tataaaagat aggtctggaa attactatat atttgtaacc acagaaggaa
    99241 aaaaattaga tccaaaaact ataaaaaatg ttttaggtgg aaaagtatct atatgttcaa
    99301 aagaagaatt aacacaaaaa acacaatgtt tgcctggatg tgtatctcct tttggatatg
    99361 agaaaaatat ctctcttgta atagatcaag atatatttaa ccatgataaa ataatctttt
    99421 caccagggat accagaaaag acatttatta ttacagctag tggatttaaa aaagtaataa
    99481 ataatataaa caatacaact tataaatata tatatattaa tgaattttaa tcataattgt
    99541 aaattagaga aaataacatt agaaatcatg cttaactata gtgtaaataa attatattca
    99601 acacaaaaga ttgatactat tattacaata gttactgtta agctcaaatt caatgtatta
    99661 tgagttatta tttttaaatt ttaattacaa aagcagggta gttttaacta ctctgcttta
    99721 tatttaacat aaaatatata aaatcaacaa aatgatttta gtctatctat tagatattct
    99781 tcgctctcaa tgcttagaac ttttagatat atacaataag tgctttcatt tgtaaaaatt
    99841 tctatagtat tatcttgttt atttacacct acaatatctt taaaattgat atgattttta
    99901 gtatttttac atttgaaaat atatctgtta tcaattaaaa tctcatcatt agtaattgaa
    99961 atatagggtt tatctcatgc tttattaaaa atttatatgt ttgttcaaga taaaaactta
   100021 caaataataa tagaaaagtt actttaaatg tacatattat cgaaaatatt atcacaaaaa
   100081 tacttataat atatatttgt ttaaaataac ctctatcgca atagtttatt aacgatatgg
   100141 aaataaaagt gttaaatcag attttggaga aataacttta aatatttcca gactcttttt
   100201 agacagcctc cataacctta tattgaacct atttattaac tttgtactta catataccac
   100261 ttatataagt ttcgtatata ttaaagtcct tatctgttac tataaaatct gcctccatac
   100321 cttgatgtat ggagccacaa acattatcaa tattacaact taaagcaggg atatatgtag
   100381 ccatttttat tgcatcttct aaagtggcta tgttccaatc aactatattt ctcacagcct
   100441 tgtccatagt taatacactt ccagctaaac tcccactatt taatcttgca acactatttt
   100501 ttacatttac attaaactta ccaagagtat attttccatc ctccatagca ccagctctca
   100561 tacaatctgt aattaaagct atgttattac tagacttagc atccatcatt attttaacag
   100621 ctattgggct tacatgatga ccatcacaaa ttaattcagc ataagtatct tttagtacca
   100681 tagcagcccc aaccattcca ggttctctat gatttaaagg actcatccca ttatatgtat
   100741 gtacaaacac tttagcacct gaattaacga cttgtttaac ttgctcaaat gtagctgaac
   100801 tatgaccaag agaaactgat atattatttt tagttgcata tcttgttaat tctattgaac
   100861 ctttacgttc tggagcaatt gctatttttc ttattaaatt tttcgatttt ttttgccata
   100921 attttaatat attgatgtca gggtctttaa aatatttttc attttgagcg cccttatatt
   100981 tttctgtaaa aaatggacct tctaaataaa tcccttgtat ttttgcaccc tcaactcgat
   101041 tgaagctatt gcttatattt tctacagaat cacttagtgt atctacagaa tcagttaaag
   101101 ttgtaggtag gaaagatgtt actccatatt ttaataagcc ttttgaaatt gtgtttattg
   101161 cttcaaatgt attgtccatg acatctgcac ctaaaagacc gtgaatgtga gtatcaacta
   101221 agccaggagc aatatattta cctgaaaaat ctataacatt atcagtaaca ttaggtcttt
   101281 cttgttgaaa ttcaccgaat ttgccattaa ttatactaag gtaacctttt tctttgattc
   101341 catgtttcat aaaaaattta tctgcataaa taaaactttt caaaataacc tccttataat
   101401 tatattatca ttgtggcatt gacgcttata atatcattct gttttcttga aagttattta
   101461 acttactatc ttacatgtct tactctatat acaaatttat cacttctagc gacactcaaa
   101521 gtaaattcaa taactatatt gtcatcatta tatgtggtac gaagtaattt cagacaaggt
   101581 gaatctcctg gtacttccaa taacttagct tccttatccg agattattcc agctgaaaat
   101641 tcttcatcag caactttaat aatctcgcca taatcttctc taaatatttc atataaaggt
   101701 ttttgttcga ttttttcttt tgtcaatcct ataaattctt tcataggaag atatgttctt
   101761 tcaagcatca taggaaggtc atcagcaagt cttaaacgca ttatttttat cattttttca
   101821 ccaggagtta tacctaattt ttcagagaaa tactttgtac tttctactac ctcaaatgat
   101881 aatacctttg tacctggctt ttttccttgt gttctcatat aatctgtaaa actataactt
   101941 tccattaagt tttgagaatt ttgacttagt gcagcaacaa aagttccttt tccatgtctt
   102001 ttgtatatat atcccatatg ctctaactca gccatagcta gtctaactgt ggttctactt
   102061 actccatatt catcacaaat ttttctttcg gaaagcattc tatcgtttgg ttccatctca
   102121 ttttcaatct tagttcttaa tatctcaaca agctggtcat atattggtct tttatcattt
   102181 ctttttatca taaaaatata gtcctttcta agtggttatt accacaattt gattatagaa
   102241 tatagaatta tttatgtcaa tgaataaagt ttaatcaaaa ttcaataagt aaggctatta
   102301 atttattata aaattatggt acattgacaa aacaagtaaa ataatttata ttcaaattgg
   102361 taataaccac tttggatata ttatatgagg tattttttat tttacagatt aatttattgt
   102421 ttagaaaagt aaaaattaat aatgaggaga atctagtatg attacagtaa taacattcaa
   102481 cccatctata gatagaatgt atagagtgaa taatataaac ataggtgagg tgcaaagggt
   102541 agtaagtaag aatgcaactg ctggaggtaa aggattaaac gtatctaagg tatgtaaaat
   102601 tttaaaagaa aatcctttag ctatgggatt tctaggcgga tttaatggag aatttataaa
   102661 aaatgaactt agaaaactag atatacaaaa taaatttaca aaaatagagc aggaaactag
   102721 aaattgctta aacataattg aggacaataa agttagtaca gaatttttag aaaaaggacc
   102781 aatagtagaa aaaagcgatt tagagagatt tgaaaatgat atagaggaag tactaaaaaa
   102841 tactaaaata ctggtagctt ctggtagtta ttgtgagaat atgccgatag actattatga
   102901 aaagatagga aatatatgta gagcaaataa tataaagttt atattagata catcaggaga
   102961 agcattaaaa atagctttaa aatcaaaacc atatttaata aagccaaata tagatgaaat
   103021 aagacattta ttaggaataa atatagaatc aagagaggaa attatcttgt caggaaaaaa
   103081 acttattgaa atgggtgcag agaatgtatg tatatcttta ggaaaagatg gtatggtgta
   103141 tttaaacgaa gataacgtat attatgtaaa agtaccagtt gtaaagtgta taaatactgt
   103201 aggtagtgga gatagcacag tggctggatt tagtgtaggt attttaaggg gttataaaat
   103261 agaaaattta cttaaatttg caaatgcttg tggtataagt aatgctttaa atatggaaac
   103321 tggatttgta gatttgggag aaattgataa atatcaagat ttagttgaag taaaaaagtt
   103381 aagttaaata catttaatgg gaatataata aatttaatgt tgttgtaata gattttaatt
   103441 ttaatataaa agatatagtc ttatataaaa ataggttaga aaaattcatt tttaacctat
   103501 ttttataata aatatctatt ttactgaatt tataattttc tctagtaatc tttgagcctt
   103561 gatacactct tctccactga tagctggttt tatattattt tctatacaat caataaaatg
   103621 atttgatata tcctcaaaac ccttttgttt taatatattt acccatgcac cactttgctt
   103681 taaagttagg tttccacctt cttctatttc cagtacatta agattcttaa ctctttgaat
   103741 cttacccttt gaaagaattt caacttgttc taactttgtt ccagaatctc tatgcataga
   103801 ggtgtttata gagaaatttt tacctttaag tttatgactt acaaatataa gattcttatt
   103861 gtctgttaaa aataaatcct ctccactaat ttcaacatct tcatttgcta accataaagc
   103921 agtgtcaata acatgtatat agtcatctat aagagtactg tcgaatttaa catttcttaa
   103981 ggaatttaat ccatgtttac atatattgat agatacaatt tctgtagcat tatttttaat
   104041 ttctttgtac ataggacaga atctacggtt gaatccaacc attaaattta agtttttttt
   104101 cgtacttaat tcgataagtt cttctccttg acttactgtt gaagcaagag gtttgtctac
   104161 atatacatga acacctaaat ttaataatat ttttataatt tcgtagtgtg tttctgtaga
   104221 gctatgtaga aaaatacaat cacatttttt agctagggat tcaatagaat cgaattgcat
   104281 gattctataa tcactacaga ctttttccct tttaacttta tttggagtaa aagctcctac
   104341 aaactcaaat ctctcagact tagttaatat aggtaaatat gccttttgag ctatggagcc
   104401 aagacctatc attcccattt ttatattttt cataatactt tctcctaagg tttttatatt
   104461 tacagaattg attataattc agacacataa atttgtaaac tttgtatcaa aaaataaatt
   104521 taaagcttat ataagtaaat tagcgaaata agaaatattg ataaagtaat tttgtaggat
   104581 atctataaaa ttgattgata taaatatttt atgtattgac aaaaaattca aattaattta
   104641 tatttggatt agtggtaatt accactttgg tctgaacaag tttttatcgt aaaataaaaa
   104701 tactaaagta tatagagtat gattttatta aatttttaaa gataaactat aaaattaatt
   104761 aaaatctata gtgtatattt tatagtgagt ttaagtagta ctaaaaatct aagcaaaaaa
   104821 ttataaaaga gtatattttt ttatagttta aagtggttat aacctcatta ccaaatgtaa
   104881 acgttacaag tatgaaagga gaatgaaaat gtttaaatta gaagaacaaa aactaaaaga
   104941 tttaggtgca ataataacaa ctaatgaaat aaaacaacag cctgaattat ggttagagac
   105001 ttatgaaata tataaatcta acaaagaaaa attaagtaga ttcatagata caataagtaa
   105061 taatcatggg cagtttagag ttatatttac aggtgctgga acttcagctt acataggaaa
   105121 ttcaatactt ccttacctta aaaataaaaa tgacataaga aaatacatat ttgaagcaat
   105181 acctactacg gatatagttt caaacccata tgattactta aaaaaagata taccaacatt
   105241 attaatttca tttgcaagaa gtggaaatag tcctgaaagt ttggctgctt taaatttagg
   105301 gaataagatt gtagataatt tttatcattt agctataact tgtaatccag aaggagaact
   105361 agctaagatg actaaaaacg acgaaaataa ttacttgtta ttaatgccat caaaatcaaa
   105421 tgatgaaggc tttgccatga caggaagttt ttcgtgtatg atgttatctg ctatgttaat
   105481 attcgatagt ttagaagatg atgtagaaaa gtcatatata aatgcaatag ttgaaatggg
   105541 aagaaatgtt atagatagaa aagatgaaat acatgagtta ataaataaag attttgatag
   105601 agttgtatat ttaggttcag gaggattagg tggtttgaca caagaagcac aactaaaact
   105661 tttagaactt acagcaggaa aaatatctac agtgtatgat tctcctatgg ggtttagaca
   105721 cggaccaaaa tcatttattg atgaaaatac acttgtattt gaatttgtat ctaactgttt
   105781 atatacaaga aaatatgatt tagatgtttt agaggagata aaaagggata aaattgctaa
   105841 attcacttgt gcagtgtcag tagaaaatga aaataacttc tcaggaacaa agtttgagtt
   105901 taaagaaaag tataataaat taccagatgt gtatcttgca atgccataca tattgtttgc
   105961 acaaactata gcattgtttg tatcggtaaa ggttggaaat aagccagata cgccatcagc
   106021 tacaggaaca gtaaatcgag tagtaaaagg tgtaacaata tatgaatatt aaaagataag
   106081 attaaaatat tataactaaa taatttggga gggtatcaat gtggcattag taacaacaac
   106141 atcaatgctt aaaaaggcac aagatgaagg gtatgcagta ggagcattta atgtagaaaa
   106201 tatggaaatg gtaattgctg ttatagaagc tgctgaggag ttaaattctc cagtaatatt
   106261 acaaactact ccatctactg taaaatatgc aggtcttgat tattatctag caaatgcaaa
   106321 ggttgctgca gaaagagtaa agatacctgt agcaatgcat ctggaccatg gttcaagttt
   106381 tgaccttgca atgaaagctt taagaagtgg atatacatca ataatgatag atggttctca
   106441 tgaaagcttt gaaaataata tatctatatc taaatcagta gtaaaagcat gtagtccttc
   106501 tcttatacca gtggaagcag aacttgggaa agttggtgga aaagaagatg atttagatgg
   106561 cgggaatagt aatgattata caaatccaaa agaggcaaaa gagtttgtcg atagaacagg
   106621 aatatcttca ttggctattg ctataggtac tgcacatgga ttgtataagg gtgaacctaa
   106681 aatagattta gataggttat cagaaattaa aaatgtagtt tcaatacctt tagtattgca
   106741 tggaggttca ggaatacatg atgatattat aaaagaagct ataaaaagag gtattgcaaa
   106801 agttaactat gcaactgaat taagaattgc atatagtaaa ggggttaaaa aagttttaga
   106861 tgaagattct gaggttatag accctaaaaa gtatggatta gcagggcttc aatcagtaaa
   106921 ggattttgtg aaagaaaaga ttaaagtgtg tgggtctgta aatagagtat aaaaataaaa
   106981 tatcctaaat ttattttaga taagtttagg atatttgtta tatgaaaaat ttatattaat
   107041 tatatgtaaa tggtgtattt ttttcattta attgatgaaa aatatcagct tttattggta
   107101 aagtattaca tatataatta aaatatacta aaaattgaac tatatatttt attattataa
   107161 aatatttata ttaaattgtg gaattaaaat atactaggat ataaaattct taaataaaaa
   107221 gaaaagattg tttttataaa aaatattttt tataccactt aaaaaaacac aataataaca
   107281 ctgagggagg tggggatatg agagaacgca ttattcttga aacattaaaa aatagtagtg
   107341 gttatctaaa catagattat tttgcagata agttgggggt atctactcga actatcagaa
   107401 atgaaataaa aaagatagaa actataaaag aatgtaatgg ttttaaatta gaatataagt
   107461 ctaaattagg atatattcta aatattaaag accaagataa attcgagcat tatctaagaa
   107521 acttaccctc agatttagtt gaaaatccag aacaacggtt agagtctata atagtagaac
   107581 ttttagtaaa tgaaggttat aaaactatag aacagttaag taaaaaattt ctagtaagtt
   107641 catcccaaat aaaaaatgat ttaaaaaaga tagatgaaaa gataaaggat acagaattaa
   107701 aactagagcg aaaggctcat tatggaatta aaatagaagg ttctataaaa gaaattcaaa
   107761 gtatattggt ggatagttat tttagaggga atagaaatat aacagagtac aggaataagt
   107821 ttatagataa tttaaaatta gctaatatta gaagtacaat aaaaaatgta cttaatgaac
   107881 atgatttaga agcaaattta acagaacttg aggaaatatt agcacaaatt attatattgt
   107941 atataagagt tagtatgaga gtagcgggaa atgctagcga tttaaaatta tatacagaag
   108001 atttaattat agataaatta ttagataaaa tttttagaga taaaaaatat aaccttaatt
   108061 atgatgaaaa gtattattta aaggagctta taaaactaaa gactaaagat aagaaagcca
   108121 ctataaagaa tattgataag aataaacttc aagatattat gttcgaattt tttagagaga
   108181 tagataaaaa atataactct aattttttag aagataaaga gttctttaac ttattttact
   108241 tacatattgc atgtttaata gaacgtataa agaagaacca caagataata aatccttttt
   108301 cagttaaaat aagtcaacag tatccaactg tttttaattt atctattcaa ttttcaaaaa
   108361 tcatagaaaa tgagtatcat ataaaaataa gtcaagatga aatagggttt atagctactc
   108421 acattgctgt tccttttgaa aagagagaag aagctaattt taataaaaaa tataaaatag
   108481 caataatatg ctcttctggt ggaggtagtg catttttgat aaatttaaga cttaaagaaa
   108541 tttttccaaa tgctgagata agaaattttt cattactaga agaaaaagct gtaatagaat
   108601 ttgcaccaga tttgattttt tcaataacaa atcttttatt tgaaataaat gctccagtta
   108661 tacttataaa tgaaatttta gacgaattgg attatttaga tattaaagaa agtgttagat
   108721 ttgcagacca tataggaaat atatcaagcc caaaacaata tatattaggt ctatttgata
   108781 agaatcactt taggtgtata aaagataaag ctaagtataa ggatatcctt gatagtatgt
   108841 caaaatgtat agttgacgag ggcgcttgtt ctccaaccta tcccaaagat gtatgggaaa
   108901 gagaaagcta tttgagtact atttatacaa atggggtggc tatacctcat ccaatcgaga
   108961 tgacaggaaa caaaaatata atatcagtag ctttgataca aactgatatt atttatgaaa
   109021 atagggtgcc taaaattatt ttcatgattt cattaataaa gggaaattta gaacttcata
   109081 agcaaatatc taaatattta agtaagatta tggcaaataa ggatatggta gacatgctaa
   109141 ataagtcaca atcttatgaa gagtttatgt ataagttaaa aatctatctg ggaggttaga
   109201 catgaatata gagtttttta agaggttgtc agaagcagat ggtatagcat ctaatgaaga
   109261 agaggttaga catgttttat tggaagaact aaaagcttat agtgataaaa ttatctgtga
   109321 tgggctaggg agtatcatct tttctaaaat aaaagatgag agtgcaccaa acgtaatgat
   109381 ttgtgctcat atggatgaag ttggatttat ggtgagaagt atagacaaac ttggtatgat
   109441 tcacttaata acaataggag gagtaaaacc tcttgcacag tttgtacaaa aagttagaat
   109501 tacaacaaaa gaagggaaga aaatccctgc tgtcataaat gctacatata ataatggtaa
   109561 ggctgaaaat atatatgcag atattggagc atatacagaa gaagatgtat ataacctagg
   109621 gattaatgtt ggggatatgg taacatatac aacaagcttt gaggaattta ctttaccaga
   109681 ccgattagtg ggaaaggctt ttgatgatag gataggatgt tttgtgatgg gagaagtttt
   109741 aaaagaactt aggaaagaaa atctaaattg taatattcac tttgcggcta caagtagtga
   109801 agaggttgga ataaggggag caaagacatc aacacagtta ataaatccag atatagtatt
   109861 tgtaatagat gtagcatgtg ctaaaaatga gtttgtgaga gactatacaa atcagaaaca
   109921 aattgataaa ggtgtaatgc taatgcatag agataggact ctagtcccta gtagaaaaat
   109981 gatagattat gtaatggaaa ttgctgataa aaataatata ccacttcaac atgatatgtt
   110041 tgaatcaggt gggactgatg gaggagaagc acatctcgta aatgaaggga aaccttgtgt
   110101 agtaacttgt gtaccagtta gatatggtca ttgtgcattt tcaatagtaa gtaataaaga
   110161 tttagagaac attataagat tgtatacaca gttaatttta aattttgatg aagaacaata
   110221 taaacatata agaaatttta tctaaaggag aataagaaaa tgtcaacaga agtacaaata
   110281 atggaactta tatcaactgc aggagagagt aaatcgaaag cttttgaggc tttaaaaaaa
   110341 gtgaagtcaa aagattttaa aggagcaaaa ttgttaatag aggaggctcg taaaatagat
   110401 attgaagctc acaggataca aacagaatta ataactcaag atttaaatga agatgttgaa
   110461 agtccagcaa tgagtctttt aatggtacat gctcaagacc actatatgac ttctcagcta
   110521 gcaaaagact tgatagaggc tttgatatat tgtcttgata agtaattgta atatagaaat
   110581 agattattta tgtaaaaatt cttttaaaag tttagatgct tttaagagat aataaaaata
   110641 caaactttag gaggaattat tatgaaaata ttattatgtt gtgcaggtgg attttctact
   110701 aatatgttaa tgcaaaatat gaaaaaggtg gtaaaagaga gtgctaaact caatgaaaaa
   110761 gattttgagt ttacagctat accagcagat tcaatagaaa gtgaagttga tgattgggat
   110821 atagtactta taggtcctca aatagctcac aaaatagact tcttaaaagg aatattagag
   110881 ccaaaaaata agccttttgc agtaatagat aaagatgtat atggaaaaat ggatggagct
   110941 acagttatga agttggcatt aataacttat aagaaacatc aattagctca aggataataa
   111001 aaataatgag ggaaaaggga gattagatat gttttcaaaa tttgaagcat ttatgaatag
   111061 agtatttatg cctttagctc ataaggttga caatcaacgt caccttggtt ctataaaggc
   111121 tggtatggtt gctatgacac cattcacaat attaggaagt atatttgcaa tacttccagc
   111181 acttccaaat atgcttggag aaaataatcc aataagcaaa ttcatactta acaattcagc
   111241 aatattggat ttaccagtaa gtctttccat aggtttaata ggattatatg cttgtatgtg
   111301 tattgctttt aatctgggga actactataa attatatata ccaggttgtg ttgcactatc
   111361 aactttttct tttttattcc tagtagcatc atttacacca gatggagctt tagaaatagg
   111421 aaatttagga gctaaagggc tatttacagg tatgatagca gggcttggtt ctgtggagtt
   111481 atataatttt tgtaataaga agaatatcac aataaaaatg ccagaaaatg tacctgattt
   111541 tgtatctagg tcttttgaat taatacctac tactataata gtttgtggaa cttttatagt
   111601 tgctcgtttt atttcattaa aagtatttgg agaattacct ccacagatac ttactcaatt
   111661 tttatctcca ttagtaggaa gtatggataa cccatggtca gttcttgcac ttaactttgc
   111721 tatatgtact atattctttt ttggaataca ttcatcagta tttagtccta taactagacc
   111781 tataatgatt acatttatag ctgaaaacat agcagctatg caagctggag aaacaatacc
   111841 tcatttcttt acagcaggag cttcaagtgc attctttgga tttacaggat gtggaattag
   111901 tataggttgt gtaattgcat gtatgttatc aaaaaatgct tcctataaga agattggacg
   111961 tgtatcatta ttccctgcat tatttgggat aaatgaacca atattatttg gagcaccgat
   112021 aatattaaat ccaataatgt ttataccaca tgtatttggt ggagctataa taggtacact
   112081 accaatgttt tttatgcact ggggatggtt agcaaagcca gtatttgacc ctccatatgt
   112141 aggggtattc ttagaaggat ttttaactaa tggagattgg cattctatat tagcaaatgc
   112201 attgcaatta gtattagcag ttttattata ttggccattc tttaaaataa tggaacgtca
   112261 acaaaataat aaaaaacaaa aagaagaaac taagaatata ttctctaaag aagaagaaga
   112321 cttattggat gatttaggat tggatttcta atattttgtt atgaataata caatggagag
   112381 attagtatcc ttaatctata aaagaggtgt tgacgctctt ttgattaaag caaaaaatac
   112441 taaaagatat ataggagctt tatcaggtag tggtgtatat gtgcttgtta ctaaggataa
   112501 aaattatcaa attctagatg gcagatatat tgatgaagca gataaaaaaa cgactggttt
   112561 cataaaaagg attgtatctc aaggttctta tataccaact ataattgagt tattaaaaga
   112621 attaaatata aaaaagttag caatagaaaa ccaagctatg agtatacaag agtatctttc
   112681 actaaaaaat gaaggttttg aaatcaattt aataacaaat gaattatgga agctaagggc
   112741 tataaaatct aaagaagaaa ttgaacttat taaaaaggct tgtgaaatta cagatgaagt
   112801 atttgatgag gttatttctg aaattaaaaa agatatgact gagctagaag tatcagctct
   112861 aattcagtat catgcattaa aaaaaggtgc aagtggaatg tcttttgaaa caatagtggt
   112921 atcaggagaa agaggtgcaa tgccacatgg aagacctaca aacaaaaaat tgaaagtgaa
   112981 tgaagctata acaatagatt ttggtgttgt ttatcaaggg tatcaatcag acatgactcg
   113041 tacaatatct ataggtaaac ctcctaaaat tattaaagag atatatgatg tagtattgga
   113101 agcacaatta tctgcaatag agtcaataaa ggaaggaact agggcaagtg atgttgacaa
   113161 agtggctaga aaaattatag ataaacatgg atttggcgaa tattttaatc atggactggg
   113221 tcatggtata ggtcttggag atggtgaagt ccccacttta aatccaaata gtgaagatat
   113281 attagtggaa ggaatggtaa tgtcttgtga gccaggtatt tatattccaa atgtaggggg
   113341 agttagaata gaagatgaca ttgttataat agatggaaaa ggtgttccac tcaataagac
   113401 atccaaagag tttataatat taggagagta aaattatgaa atataatttt aataaagtat
   113461 taaatcgtaa agaaggaaat tgtagaaaat ggagcaacca tgtgattaaa gaaaaattcg
   113521 gcttaaatga agatgcaatt cctatggatt tagctgatat cgattttgag tgtgctcctg
   113581 ctataaaaga atctatctta aaaagagcat cagtaggaga ttatagttat acttttatag
   113641 gagatgactt ctatgatgcg attataaatt ggaataaaag aagatttgat gtatatatag
   113701 aaaaggattg gataaaatta acttttggta ctgtttcaac ccttcattat atagtccaag
   113761 catatactaa agaaggagag ggtgtattga taaatacacc tgcttatgac ccttttgcag
   113821 aggctgtagt aaataataat agaaagttgt gttgtagctc attaaaatta gataacaata
   113881 gatattattt agatttagag gatatggaaa atcaaatgaa atacgagaat ataagattgc
   113941 ttatattctg tagtccacaa aatccatcag gtcgtatatg gacaaaagaa gaactatatc
   114001 aagtgtcaga gttatgttta aagtataatg taattttggt aagtgatgaa attcatagag
   114061 atataatatt taaagattat agatttgtat ctttatggaa tgctcatcca gaaatttata
   114121 aaaatagtat aatctgtgta tctcctaata aaggcttcaa cttaggtgga ttaaaaacat
   114181 catatgtatt aataagagat gtaaaaatta gggagacttt attagaaaga ctaaaaagta
   114241 attcaataac ttctccaaat gtatttgcaa taccagcaat agttgcagca tataatgaaa
   114301 gcgaagaatg gctagatgct atgactagtt atgtagagga gaattttgaa atagtttatg
   114361 atttctttga aactaatata ccaaaagcta aagtgatgaa gtcagactct tcttttttag
   114421 cctggataga tgtaagagaa gtatttagaa atgaagaaga atctaaagaa ttttttagac
   114481 atgcaaactt aactatggta gttggaagtt attttgtgaa agatggagat ggatttattc
   114541 gtattaatgt ggggtgtcct agagaaacat taaatgaggc tctaaataga atcaaaaaaa
   114601 cgtatataag tatgtattgt taaatcataa aaaaagtatc tatggtttat ataaaaacac
   114661 attatttagt tatcaaatat tactggaatt ttgtattcca gtaatatttt ttgattaagg
   114721 aatattattg aggaatatta aataatatta tttcataccc ttatattaac ttatataaaa
   114781 atattttatt attttgggaa cgggttagct aacctttatc tagtgtatat ttatattgta
   114841 agaagcagta aaaacttttc ctttactaag tttattaata aacttcttat ctttataaat
   114901 tttgttagaa tttatactat cagcaagacc ataccaaggt tctatacata aaaaaggagc
   114961 agtagaatta gtttcactat aataaggagt ccaaatacca actagaggga aatcttccat
   115021 agatatagtt acatatttac taccatcatt attacatata gatacttcat ctatgttggt
   115081 gtatatcaaa gcatcattct tgaatacttc tggatttaac tgtaagtttt ttaattttcc
   115141 aataggtatt atatcatctg taaaagaacc atttaaattg attttttcta catcattttt
   115201 acttttaaac tcaaggtagt gtttagaaaa atcattttgt tcgtaaaaag gaaggttgaa
   115261 tgcaggatgt ccacctatag aaaagaatat atctttagaa tcagtatttt ttacaatcca
   115321 ttctacctca atactattat catttaatgt atagtttatg attagctcaa aagaatatgg
   115381 atacttcttt aaagtagatt cattatctgt taatttataa gttacaaatg tatctccttt
   115441 atctacaatc tcaaagtcca tatctctagc aaacccatgt tgagtcatgc tatataaatt
   115501 ttcttctatt attgtttcat tatcaaaaag tttacctact atagggaaca atataggtga
   115561 ttgtctaccc caatattttt tattaccatc ccaaagaatg tttttgtcgt attttttaga
   115621 aaatatttta gttagttcag caccaaaact gcttgattcg ataattaaat tttcattttg
   115681 taatatattc atttaaaatc tcctttatta tattttatga gtatattata ctataaagta
   115741 taaagattat taaattttat ttgatattat atttgaaagg tagattttag tatattttga
   115801 aaattttgtt ttgaaaattt gggattattt aagaaatttt gtattataaa tagaaggaaa
   115861 tagataaatt gtatagaata aaatatatta agataagtta ataatgttaa atgggaagtg
   115921 agtttatgga aaaaccaaat acaaaagttg ttaactttca agagtataaa gacagaaaaa
   115981 accaaagaga agtaaataga gacgaacttt taaagcttat aaaaaaaatt gtgacttcaa
   116041 aatagggagt tgttaaactc cctattttaa tgggtttcat aataattaag acattctgag
   116101 tccatctata taatctttga attgtgagaa atctgattca ctacttaatt ctatggtttc
   116161 tgaaccttcc gtataaaaat tcttaagata gatagtataa ttattatcaa cacaatcatg
   116221 ataaatacat ctatatccat cttcatacaa actttttaac ttagaaaaat catccacaat
   116281 atcatctcct taaagttctt ttaaatatat ttttacaaaa agcatctaat tgtatgcaaa
   116341 ttgacaatgt aaaataaagg aaatgaacat aaaatgtaga ataattttta tatgtatgta
   116401 aaaataaaaa gaaaaagttg aaatttttag aaaataagag tattttttat ctaaaaagag
   116461 agtttaaaaa ataaaaaaat gttgggagga taaaaaatga gtttattaga aagtatatca
   116521 aaaaacatat attccctaga taactcttct attgaaaaaa caaagcagag attggatagg
   116581 cttatacatc caactggaag cttaggaaag atagaagata tttgcatgca attagcagga
   116641 atatttggca atgaaaattt tgacacaagt aaaaaggtta taattgcttt tgcgggagac
   116701 catggtgtat atgaagaagg cgtagcacct gaccctcaaa atataacaaa attacagttt
   116761 ccaaattttt cgaaaggtct atgtggagtt ggagtaataa gtaaatttgt aggggcagat
   116821 gttgtagctg tagatgtagg aataaactgt gatgaaaaac tggatggggt attagactac
   116881 aagataagaa aagggacatc aaatatggca aaaggtccag ctatgagtaa acaagaagca
   116941 attagatgtt tagagatagg tattgaaata gcagagcagt gtatagaaaa agattataag
   117001 gttattggta ttggggagat gggtatagca aatactactc caagtacagc aataatatct
   117061 gtaatttcag ggtgtgaccc gttagaagtt actggtatag gtgcaggtct taagaaagaa
   117121 agactaaagc ataaggctga agtaattaga aaggctattg aaattaataa tccaaatcca
   117181 actgatggag tagatatttt atctaaagta ggagggtttg aaataggctc aatggcaggt
   117241 gttatattag gttgtagtgc taataggatt ccagtagtaa ttgatgggtt tatttcttat
   117301 gcagcagcat taattgcata taagataaat ccaaaaacta gagaatatat gatagcatct
   117361 catttatcag cagaatcagg aactaaaaga gcattagaca tattaaaatt agaccctcta
   117421 ttaaatatgg atatgagatt aggtgaggga agtggagctg ctttagcatt tgatataata
   117481 gaagcttcaa actatactta taaaaatatg gcaacttttg atgaaattga catgggaaga
   117541 taaatataaa aatttaatat gtaggagtgt tttgatgagt aaaattattc tagtaacagg
   117601 aggagctagg tcagggaaga gtaactttgc agaaaaacta tgttttgaca gaaataataa
   117661 tacagcttat atagcaacct ctattccatt tgatgatgaa atgaaagata gagtaaaaaa
   117721 acatcaagag aatagaccta gaaactggaa tacttatgaa atatatgaag atatatattc
   117781 cataattaag gagatatata aagaacatga aactgttata ctagattgtg taacattatt
   117841 agtaaataat ctaatgtttg aacacaatct aaatatagaa gaatctagtc aagaagaaat
   117901 aaacaaatta gaacaataca taaaagaaca agttgtcaaa cttattgaag aaatagaaaa
   117961 aacaaatcta tactttgtat ttgtaacgaa tgagttaggt atgggaattg ttcctgggaa
   118021 taaacttagt agaatttatg ctgatattgt aggtcgtata aatcaatata tagcttcgaa
   118081 aagtaatgaa gtttattttg tagttagtgg catagctatg aagataaagg agtagtatgc
   118141 aattatgaaa agatttattt taatactaca gtttttgaca aggataccta taaaattaaa
   118201 tgtaggattt gatgatgaat tttataagtc aatagtatat tttccacttg taggttttgt
   118261 cataggtata ttatcctatt taataggatg gatttctatg cttttatttg aaccatttat
   118321 agcttctata ataataactc tagctggtgt cttaattact ggaggacttc atatagatgg
   118381 attaggagat acatttgatg caatatatag ttatagggat aaagaaaaga tgcttgaaat
   118441 aatgaaagat tcaagattag gtacaaattc acttctagca attatgtttg tacttttatt
   118501 aaaggttgga ttcgtttacg atataataag taataattca ttgtgggtta taatttttat
   118561 gccaatgatt gcaaggttag gagtaatgtt attgacatat aaaactgtga caccaagaga
   118621 aaaaggaatg ggaaatttat ttataggaaa attaactaca agtatgttaa taactgctat
   118681 aatatacaca ttattgatag ttgctcttat tactaaattt atatttttat tacctaatat
   118741 agtgttgatt aaagtattag gttcgattat agtggttttt gtatttataa tattattcaa
   118801 aaaacatatt tataaaaaaa ttgacggagt aacaggagat atattaggct gtggaataga
   118861 actttcagag cttgtatatt taatttacat atatttgtta atttttatgt ttttttaaag
   118921 taaaaagtgg tgataatatg ataagattaa tattaataag acatgcactg acaaatgata
   118981 ataaaaaagg aagattatca ggccatataa atagttgtat tagtgaagag ggcaaactac
   119041 aaataaataa aattaccaga tatctgagta atgaaaacat agataaggtt tatacaacac
   119101 catctacacg tactaaagat acagtagaaa aaatatcaaa attgaaatta ttagaaatag
   119161 aagaaaaaga agctttaaga gaaattagtt ttggagactt tgaaggaaga acttttgaag
   119221 aaattaaaat caaatatcca aatgagtttg aaaaaatgat aaaagaaggt aacaactata
   119281 gatatccaaa tggagaaagt ttgatagact cttataaaag agtggcaaaa gaaattgata
   119341 atataatttt agaaaatgat agtaatttag atacaaagac aatacttata tgttctcatg
   119401 caggaacaat aagaaatata ataacacatt tgatttctgg tagttataag tatcactgga
   119461 actttaaaat tgataatgca tctattacag ttttagaaat tgatggtgga tttgctgtaa
   119521 tagataaaat gaattttaca gacttcatat aatttaatca aaaccaaaca ttacaatctg
   119581 tgtttgtaat atatctgtaa aattagcaat gaaaatagaa tattagatta aaattaggtt
   119641 cttaattaga tgttaaaagg gaaaaaggtt agatgccttt gcagcccccg ctactgtgaa
   119701 accaacgaaa ctataaatac cactgtcagt ttgatgggaa ggttatagaa gtaggatgaa
   119761 gttaagccag gatacctgcc taatttaatt taaaacaaga ggtcttcggg gtgaaagatt
   119821 tttaattaat atatataaaa gtcctatctt tagaagtatt aggacttttt ttaatttgtt
   119881 ttttagtaaa ttttaacaag gagggatgga aagtggggaa aaaaataatg ctacaaggaa
   119941 cagcttcaaa tgtaggtaaa agtgtcttag tagcaggttt gtgtagaata tttaagcaag
   120001 atggttatac tgttgctcct tttaagtcac aaaatatggc tttaaattct tttataacta
   120061 aagaaggatt agaaatggga agagcacaag tatttcaagc agaggctgcg aaaatagagc
   120121 caatagctga tatgaatcca gttttattaa aaccttctgg aaataataaa agtcaagtta
   120181 ttgtaagagg taaagttgta ggagaaatgc cttcttctaa gtatcatgat tataaattgg
   120241 agttagtaga tgtgttgaag gaaacattta atagcttaaa ttcaaggtat gatattgtag
   120301 ttatggaagg ggcaggaagt aatgcagaga taaacttaaa agatagagat atctctaata
   120361 tgggtatggc agaaatagct gatgcacctg taataatagt cggagatatt gatagaggag
   120421 gcgtatttgc ctcattagca ggaacaatgc ttttattaaa tgaagatgag aaaagaagag
   120481 taaaaggtgt aataatcaat aaattcagag gaaaaaaaga gcttttacaa gatggtgtaa
   120541 agatgctaga agacatcata aaagtacctg ttataggagt agtaccttat acagatataa
   120601 agatagaaga tgaagacagt gtaactacga gatttaagaa aaaaatgaat aaaagtgata
   120661 tacacataga aataattaga actccacata tgtcaaattt tacagatttt aatatatttg
   120721 agacacaaga agatgtaagt attagatatg tagactatgg agaatcttta ggaaatccag
   120781 atatattaat aatacctgga actaaaagta ctatagacga tttaacatat ataagaaaaa
   120841 gtggtcttga atcgcaaatc aaaaatttac atagtcaagg taaattgata tttgggatat
   120901 gtggtggata ccaaatgtta ggtaaaaaat taaaagaccc ttatcatgtt gaaagtgaaa
   120961 ttgaagaagt tgatggaata ggtcttttag atacagaaac aacgtttgag aaagaaaaga
   121021 ctacaacaca agtagaagct actattgttg aatgtattag tgaatacatg aataacctta
   121081 agggaaaaaa agtaaaaggt tatgaaattc atatgggaat tacaaagcga ggtaatgatg
   121141 taagaagttt agatttaata actaaaaaac ttgataaaga agtgaattat actgagggaa
   121201 gtgtaaataa agaggggaat gtaattggga cttatcttca tggaatattt gatgaaataa
   121261 gctttacaag agcaatttta aacaatataa gaaagaaaaa aggtttagga gaattagaaa
   121321 gcaaagtaaa ctcttttaat gaatttaagg ataaagagta tgataagttg gctgattttt
   121381 taagagaaca tttagatata gagaaaatat atgagattat gaaataatga tgacctttat
   121441 ttataattta ggtaggaggt gtattatgaa aaaaatttta attgcgggta ctaatagtgg
   121501 agtaggtaaa acaacaatat cattaggaat aatgcaagct ttggttaaga gaaatatgaa
   121561 agtacagcca tataaagtag ggccagacta tatagaccct tcatatcaca cttttataac
   121621 tggaagacat tctagaaatt tagattctta tatgttagat gatgaaaaaa taaagtatat
   121681 agtaaataag tcctcaaaag atgcagatat ttcagtaata gaaggtgtta tgggcttata
   121741 tgatggcttt ggaatagatt tagataattg tacaagttca catacttcaa aggtattaaa
   121801 gtcacctgtt atattagtta taaatggtaa atctatggca gcatctagtg ctgctatggt
   121861 acttggatat aaggaactag ataaagatgt aaatataaag ggtgttatag taaataatgt
   121921 aaagactaat agccattatc aaataattaa gagttctata gaaaaatatt gtaatgttga
   121981 agtattgggg tattttcctc caaataacaa attctctttg gaatctcgac atctaggatt
   122041 agttcctagt gtagaaatga aatcactaag ggaaaaattt tataccttag cagatgaaat
   122101 tgaacactat atagatattg acagaataat tgaaatatca gaaagcgatg aatttgagag
   122161 tgattttgat tttcaaactt taatagaaaa taataaaata aaagaacatg ttaataataa
   122221 atcaatagct atagcatatg ataaagcatt caatttttat tatagagaaa atatagagct
   122281 tttagaggaa cttggtttag aaataaacta ttttagccct cttgaagatg tatcagtgcc
   122341 aaatgcagat tatatttata ttggaggagg ttttccagaa atatttggaa aagaattaga
   122401 agcaaatgag tcaatgagaa cttccatatt agaagcacat aacaataata tacctattta
   122461 tgcagaatgt ggaggactga tgtacctagg tgagaaacta ttgaatcaag aagaacaaga
   122521 atttaatatg gttggaatat ttagtggaat tagtaagatg acatcttctt taaaaagatt
   122581 tgggtactgt attggtgtag ccaaggaaaa tactctttta tctaaaaaag gggaatctat
   122641 taaagggcat gagtttcatc attcaatatt tgaaagtgat gaaaaatgtg tatattcaat
   122701 gagaaaagaa agagatggaa atatagtaga agagtgggat ggtggttata gtaaaggaaa
   122761 cactttggct acatatcttc atactcattt ttataataac ttaaattgta tagaaaattt
   122821 tttgaaaaaa ggaaatgaat aaatgagtat tttatctatt tatataggat atgtgacaga
   122881 cttaattgta ggagacccat attcttttcc tcatccagtg agatttattg gtaaacttat
   122941 aaattttact caaggtataa taagaaaggt ctctaaaaat gataggcagt taaaattggg
   123001 gggatttata ttatggttta taaccgttgg aataacatat ttaacaactt atgctatagt
   123061 gaagttattt agttttaata tattattgag tattgtagtg aatagtttta taatttatac
   123121 aacactagcc acaaaatgtt taaaagacga agcattaaaa atttataatg tattaaaaac
   123181 tgaagatata gaaaaatcta ggattcagct atcttatata gtaggtaggg atactactaa
   123241 tttaagtgaa cctgaaataa taagagcaac tgtagaaact gttgcagaaa atactgtaga
   123301 tggtataatt gcgcctatgt tttatgcatt tattggagga gcaccacttg ctatggccta
   123361 taaagctatc aatacattgg actcaacagt tggatataaa aataaaaagt ataaagatat
   123421 tggttttgca tctgctaaga ttgatgatat agccaattat ataccagcta gaatatctgt
   123481 cattcttatg acaattggaa gtttcttttt aaattataac tataggaact gttttaaaat
   123541 ctctattaga gatagaaaga atcataaaag tccaaattgt gctttttcag aaggtgcagt
   123601 atctggtgct ctaggaatac aactaggagg taccaacgtt tattttgggg aaaaagtata
   123661 taagccaaca ataggggata agcttaggga gatagataaa gaagatataa ttaaaactaa
   123721 taaaattatg tatgcatctt catttgtatc tatgttggta tttacactaa tatatgtgtt
   123781 atgtaggatg attttaatta acttaattat ataaattatt gtttgttaac ttaaggggga
   123841 catagatgaa ggatttaggg catggtgcca atgtagatga aatggcaaga ttatatggga
   123901 aaaacccaaa agaaataatt gattttagtt caaacataaa tccaaatgta ttaccaaatt
   123961 tagagagata cattttaaaa ggattagaag agtgtaggaa ttatccagat ataaattata
   124021 caaacttgag agaaaatata tcaaaatata tagatataaa tccagacttt ataatacctg
   124081 gcaatggtgc aacagaagtg atttatttgc ttatgaagag tattaaaaaa aaattggcta
   124141 taataaatcc gactttttca gagtatagaa gaagtgctga gttaaataat ttagatatta
   124201 tagatttaga gttagattta gaaaataact ttaaacttaa tatagatata ataaaagaaa
   124261 atataaaaag atttgatagt ttatttatat gtaatcctaa taatccgagt ggtaatgtcc
   124321 aagatttaaa ggaacttgtt catttactag ataaacataa taaggtatta ataattgatg
   124381 aaactttcat ggaatttgtt gaagatgaaa gtaaatatag tttggttaaa tatatagaat
   124441 caaataaaaa tatatttata attaaagctg tgactaagtt ttttggtatg cctggtttaa
   124501 ggctaggata tggtcttaca agcaacactg aaattatgaa caaaatttat gaacacaaag
   124561 agccatggac tatcaattct tttgcagata tactatccaa ttttattttt gaagataaag
   124621 agtatattaa aaatagtaaa gaatattaca tagaagaaag agaatatatg ttacaagagt
   124681 tgagaaacat aagaaatata aaggtctatg atacagatgc taattttatc ttgataaaaa
   124741 tatataaaaa aacaactaaa gaattgaaaa aagatttgtt taagcaagga aacatattag
   124801 ttagagatgc gtctaatttt ataggattag atgatagttt tataagagtg gctataaaat
   124861 ctcatgaaga taacaaaatt cttatagaaa atatgaaaaa tttattgggt gattaaaatg
   124921 aaatcttatg gaatatgccc agcatcttgt ggagaatttg tacaaggtat aattgatgat
   124981 gaagaatatt tatgttcata tgcaatagat atgtattcta aagtgtatat tgaagaaaaa
   125041 ttagtagata taaatttagg gagatataag tcaaggttag caattgaaaa ggtatttgaa
   125101 aaatttaatt tacctaaaaa atatactaaa aatatttctt taaatataaa tagtaaaata
   125161 cctgttggaa aaggaatggc aagttcaact gctgatatag gagctactat aaaggcaaca
   125221 ttatcattga tagataaaga tttaagtagc gaggagattt ctaaattagc agcagaaata
   125281 gaaccaacag attctatatt tatagataaa aatagtatat ttaatcctct aaatggaact
   125341 gttataaaat acctaggaaa tctgaccaat gcgaaagttg taatacttga accaaacaaa
   125401 gtactagata ctatgaaaat tagattgagg caagattata ataaattaaa agtagagaat
   125461 aaagaagtaa taaaaaaatc atttgctctt ttagaagaag gattgaaaaa aaataattta
   125521 tctctagttg gagaggcttg tactttgagt agcttagcaa atgagaatat agaaaaaaaa
   125581 gagtatttaa atgaaataat aaaaatatca aaaaaatatg gagcatatgg agtaaatatt
   125641 gctcacagtg gaactgtagt aggtatttta attgataagt ttatgaatga taaaaagatg
   125701 atagatgcct tatgtgaatc taacataaat tctgtctata ataaaattta tacacaaaac
   125761 ataattaatg gaggaattaa gggggaaata gaatggaata cataaagaat cctatgaaaa
   125821 tagaagaaaa aagttttgaa ttgatacaag aaataataga cgaaattaga ccagattata
   125881 aatttaagaa taatatcgaa gaaaaaataa taaaaagagc tatacatact actgctgatt
   125941 ttgattactt agatatatta aaaatatcag aagaagcagt tgatagtata atagatgcat
   126001 taaaaaataa tgcaagtatt tatactgata caaatatggc tcttagtggt ataaataaaa
   126061 gaaagttaga atcattagga tgtaagtata aatgtttagt ggcagatgat gaaactgtaa
   126121 aattagctaa agaaaaagga attactcgtt ctatggcagc agtagaaatt gcagctaaag
   126181 aagaaggaag aaaaatattc gttttaggta atgctccaac agctttatat aaagttatgg
   126241 agatgaaatc agaaggtaga ttagatttag atgcagttat aggagttcct gtaggttttg
   126301 taggagcaca agaatcaaaa gatgaagttg aaaaaacaga tataccatat ataatttcta
   126361 aaggtagaaa aggtggcagt aatttagcag cagcaatagt aaatgctata ttatatacta
   126421 tgtaggtacc tatatggaag aatatgtata tatagatgga aaaaaatata gaagaggata
   126481 tactacaggt tcatgtgcaa caggtgcatc taaggctgct gtatatatgt tgattacaaa
   126541 aaatagaatt aatactataa atatagatac tcctaagggg ataccattac tactaaaagt
   126601 tgataatata aatatttctg atacatttgt ggaatgttcc ataaaaaaag atggtggaga
   126661 tgatatagat gcaactcata ccatggatat ttatgcaaga gctgaaattg tagctaaaaa
   126721 tgataagaac aaagggtatc ttactctaaa ggatatagat agtttaagca ccaatagtga
   126781 gtgtaaaagt gagctctata agtttattag ggtttatggc ggaactggta ttggtgtggt
   126841 tacgaaaaaa gggcttagtg tggatgttgg taagcctgct ataaatccga ctccacttaa
   126901 aatgataaat catgaaataa gaaagcttat aggagataat tttgaatcta tacttggaaa
   126961 tgataaagtt ttaaaaataa caatctttgc tccacaagga gaaactgtag caaaaaaaac
   127021 atttaatcca agattaggta tagttggagg aatatctata ataggtacaa ctgggattgt
   127081 tgaacccatg agtgatgatg gttggaagaa gtctctatct atagaacttc agatgaaaaa
   127141 agaacaaggc ttagataaaa taatattggt tccaggaaat catggagaac agtttataag
   127201 agaaaaatta aacttagata ttaagtatgt agttagggta agtaatttta taggctatat
   127261 gattaaagaa gctcaaagaa taggatataa aaaaatctta atggcaggcc atataggtaa
   127321 atttataaaa gtatctgctg gtatttttaa tactcatagt aaggtagcag atgctagaag
   127381 tgagatatta gtggcaaact tagctttaat gggagctaga tacgaatttt taaataaaat
   127441 aaatcaatgc gtaactactg aagaagctgt agagctaata aataatagtg aatatagaga
   127501 agtatataat atattaagta ataaatgtag ggagagagta aagcaatact tgaatgaaga
   127561 ttcagatgat attgatgtgg aagtaatcat attttcaatg gataagtctt tattaggtaa
   127621 atcggataat acagatgatt tagtggaggt ctttatatga taaacataat aggtctagga
   127681 cctggtaact tagactatat aacaaaaaaa ggtgaaaatt taatttccac ttctgatgta
   127741 ttaattggtg gtaaaagaaa tttagagtct ataaaaaatt ttgaaggaga aaaaatagtt
   127801 ctggattcta atttaagaga aattatagag tacataaata acaataaaga aaagcaaata
   127861 tcaataatag catctgggga cccattaata tatgggatag gtagatatct atctaaaaat
   127921 attgataata agatgttaaa catggtttct gggattagtt cattacagta catattttca
   127981 aaaatatatg tagatatgaa tgatgtatat attacaagta gtcatggcaa agtaccagat
   128041 tttgattaca tattatcaca taaaaaagta tgtatggtta cagattcaaa gataggacca
   128101 aaacaaataa gtagagaaat aatagataga aatctaaata aaattattgt tgtaggagaa
   128161 aatctgtcat atgacaatga aaaaataaca atagcaaaac cagaagagat aataagaatg
   128221 gacaattttg atatgaatgt tgtggtgatt ttagatgagg aatagtgaat ttataacagg
   128281 taaagttcca attactaagg aagaggttag agcaatatca ataagtaagt taaatttagt
   128341 aaatgctaaa aacctcatag acataggggc tggtacagga agtgtaagtg ttgaagctgc
   128401 atataattat cctaatttaa aggttatatc tatagagaaa aatgatgatg ctatagagct
   128461 tatagagaaa aataaaaaaa aattcaactt aaaaaatata gaagtgataa aaggatatgc
   128521 tccaattaat ctagggttaa atgttaaagt tgagtctatt tttcttgggg gaacaggaaa
   128581 taatctggaa gaaataatag aatggtctaa aaaaaatctc gttacaggag gaagacttgt
   128641 agctaatttt ataataatag atacatttaa tcaaacttta aaactgctta aaaagcatgg
   128701 atttaaagaa atagatgtat gtgttttgaa tgtttctaaa ctagagaaac taggaaaagg
   128761 tgaatatttt aaaccactaa atcctatata tataatatct tgtgaaaaag gggaaaatga
   128821 tgatgaataa agtacatttt gtaggagcag gacctggaga taaagagctg ataacattaa
   128881 aaggatacaa attattaagt aatgcagatg tagtgatata tgcaggttca cttgttaatc
   128941 ctgaactttt ggaatactgt aaagaagatt gccaaataca taatagtgca catatggatt
   129001 tacaagagat aatagatgtt atgagagaag gtatagaaaa taataaatct gttgtaagac
   129061 tgcaaacagg tgatttttct atatatggtt caattagaga acaagtggaa gatttaaata
   129121 aattgaatat agattatgac tgtactccag gagtaagttc ttttttaggg gcagcatcat
   129181 ctttaggggt tgaatataca gttccagaaa tttctcaaag tgtaattata acaagaatgg
   129241 aaggcagaac tccggttcca gagaaagaat caatacaatc atatgctaag catcagacat
   129301 ctatggtaat atttttatct gttcaagaaa ttgagaaagt tgtgtctaaa ttactggaag
   129361 gtggatatcc aaaagatact cctatagcag ttatatacaa agcaacttgg gcagatgaaa
   129421 aaatagttaa aggaacttta agtgatatag ctgtaaaagt taaagaaaac aacataaata
   129481 agactgcttt aataatggtt gggagatttt taggagaaga gtataataat tcaaagttat
   129541 atgataagga tttcaaacat gaatatagag ggtaatattg ctataatctg tattacagaa
   129601 aatggaaaaa aattagctct aaagataaat tctctagtaa ataactgtgt tatatatcat
   129661 gtaaaaaata aaaaaagtaa tttagttgtt gaagaaacat gtgtgcatac agttcaaaag
   129721 aagttaagtg attttgtagg agaaatattt agcaaatttg attacatagt atttataatg
   129781 gctacaggta tagttgtacg aagtatagca ccatacataa taagtaagtt tagtgaccct
   129841 gcaattttgg taatggatga aaaaggcaaa aacgtaataa gtttattgag tggtcatatt
   129901 ggaggggcaa acagcttgac acagtacata agtagtctta ttgatgctaa tccagtaata
   129961 acaacagcta ctgatataaa tcaaaaagct tccttagata tgatagcaaa aaaactaaat
   130021 gcacatattg ataattttag agaaagtgta aaagaagtaa attctatgct agtgaataat
   130081 aaatgtgttg gattatatat tgatgaagga tatcaagttg atactagggg atttaaactt
   130141 atagaaagta aggatgagtt aagagatagt ttttcaaaca catatataga aaaattagtt
   130201 gtagtaagca ataaaataaa ttgtattgaa gatattgaga atttaaacct agataataaa
   130261 tttgaacatt gctatagtac caaaaaaata ataaaggtaa ttccaagaga tatagtgata
   130321 ggaattggtt gtagaagaaa cacagatagt gaactgatgt ataattcact tgaagagttt
   130381 cttaataaac aaaatataga tattagagca ataaacacag ttggaagtat agaactgaaa
   130441 aaagacgaaa aagcaataat tgaattatca aaaatattga aggtgccatt taaaacattt
   130501 tcagtagatg aaatatctaa ggttgataat ctttttgata aatctgattt tgttaaaaaa
   130561 aatgtgggcg tgtattcagt agcagaacct gtagcatatc ttttgagcaa aggtaatttg
   130621 ttagttgaaa aacataaata taaaggaata actttttcag tggggagaat aaaaatatga
   130681 tatacgttgt aggaattgga ccaggaagta aagatacaat gactttagaa gcaataaaag
   130741 caatagaaga ttctgaggct attgttggat ataagactta tataaaacta atagaagaat
   130801 ttatacaaga taaagaaata atccaaaatg gtatgaggca agaagttgat agatgtaaac
   130861 aagcagtaga ggaagcaaaa aaaggaaaaa aagtagcagt tgtaagcagt ggtgatgctg
   130921 gaatatatgg gatggcaggt cttattttag aattaatatc taaagaagat gaagatataa
   130981 aagtaaaggt aattccaggt gttactgcaa gtattggtgc agcagctata cttggagctc
   131041 ctattatgca tgatttctgt catataagtt tgagtgattt aatgactccg tgggaagtaa
   131101 ttgaaaaaag attgagattg gccgctgaag ccgattttgt aatttgtttg tataatccaa
   131161 gaagtaaggg aagaagtgaa catctaagca aagcatttaa aataatgggt gagtttaaag
   131221 gtggagatac acctgttgga gtggtaaaag atgtaggtag agaaaaagaa gaaaagtttg
   131281 tatgtacttt tgaaaatatg gactttgaaa aagtcgatat gactacaatg gttataatag
   131341 gaaataagtc tacctatatt aatggtgaat tgatgataac tccaagaggt tatacagtat
   131401 gattttaatt ttaggaggaa cttcagatag tttatctata tgtgataaga taaatgaact
   131461 gaaaaatcaa ccatatatcc tttcagttac tacagattat ggaagggaac ttgcacaaca
   131521 acatgcagag aatgtaattt taggtaagct tggaaaagaa gatatgctaa aatttataaa
   131581 agataataat atagtaaaaa taatagatgc cactcatcct tatgcagttg aagtatccaa
   131641 aactgctatt acttgtgcaa aactattgaa tatagattat ataaggtttg agagaaaatc
   131701 tttaattgaa gaaatagatt atgataataa atttatagta gatacaattg aagaagcctg
   131761 tgaaatagca aataaaatag ggacaaatat attcatagga acaggaagta aaaatttgaa
   131821 cagatttata gaggaaataa gtgataaaaa tttagttgtt agagttcttc caacgagtga
   131881 agtcatatta agttgtgaaa aattaggttt aagtgcagat aatatagtag ctatgaaagg
   131941 tccatttagc cagtatataa atgaagaaac atataaacat tacaatatag atttagtcat
   132001 aacaaaagaa agtggaatag ctggtgggtt cttagagaag gtaaatgcat gtaaaacttt
   132061 caatatacca gtaataatta taaaaagaga atcgattaac tacgaaaatg tatttaatga
   132121 cataaataaa attaaagaca tacttgtgta atttttagga ggagaatata tgaagaaagc
   132181 cttattagta gtgagttttg gtacaagcta tcatgaaacg agagaaaaga caatagatat
   132241 atgtgaagat aaaataaaaa attcgttaaa aagccatgat ttttttagag cttatacatc
   132301 aaatatgata ataaataaaa taaaaaaaag ggatggaata gaaatagata atccaataca
   132361 ggctttagat aaaatatatg aacaaggata tgatgaagta ataatccaaa cacttcatat
   132421 tatatgtggc gaagaattta acaaattaaa agaacaagta gatggatatt cttctaaatt
   132481 caaaaagatt gtattaggaa gacctttgct tacacatatt gaagattatc aggaagctgt
   132541 agaggcaatt aagcatcaaa ttcctcatat ggaaagtaat gaagcagtag tatttatggg
   132601 acatggaaca cttcatgaat cacattcagc gtatccagca ttggaataca tgcttaggga
   132661 taatggaata aatgcttatg ttggtacagt tgaagggtat ccagaaatag aacatgtaat
   132721 aagaagatta aaagaaggaa atataaaaac tgtaaatttg atgccattta tgttagttgc
   132781 aggagaccat gctataaatg atatggcagg agatgaagaa gattcttgga aaacaatatt
   132841 agaagaaaat ggatttaacg taagagttca tttaaaagga ctaggagaaa attcttatat
   132901 acaagataaa tttgttcgtc atgctgtaaa atgtgcagag aatgcgaagt tctatggtat
   132961 aggagcaggg ccaggagatg gttctttact tacgataaaa gcggttaata ctttaaagaa
   133021 actggatatt ctatacactc cagaatcaaa aaagggtgga gatagtctag ctttatcaat
   133081 agttagtgaa tatttacctg aatcagtgga aataaagtct aggcatttcc caatgagttt
   133141 tgatgggaat gaaaaaagtt tagcttggga caatgtggca caagaaatta tatcagatgt
   133201 taatgatggt aaaaatgtag gatttgttac attaggagac cctatgattt atagtacata
   133261 tgtatatata atgaaaagaa tattaggaga ggtagaagtc gaaactatac caggaatttc
   133321 atcattttcc aatatagcat ctaatcagaa ttttccatta gtcatggata aagaagcttt
   133381 agtggtaatt ccttgtacta tggaagatga taagatagag tatgctttgc aaaattataa
   133441 ttcaatagta ttgatgaaag tatataaaaa ttttaaagaa ataataaaaa aattagaaaa
   133501 gtatgattta atagaacatg ctattttagt aagtaattct tcccaagaaa gcgaaaatgt
   133561 gtttacagat ttaaaagaag cgcaccttga agataagata tcttattttt ctactatact
   133621 agtaaataaa gataataaaa taaaataggt gaaagcaata tttaaaggag acttaatatg
   133681 aatatagtgg ttggaactag gggtagtaat ctagcgctta tacaaacaga atgggtaata
   133741 aatgaattaa aaaagaagta tccagaaatt agttttgaga ttaaaatcat aaagacaaaa
   133801 ggagatttaa ttcaaaatgt ttcactggat aaaataggtg ataaaggatt gtttgtcaaa
   133861 gaaatagagc aacaattatt agatggaaaa atagacatag cagtacatag tatgaaagat
   133921 atgccttctt atttagcaga tggactaaaa tttgctcata cgcctaagag agaagaccca
   133981 agagatgttc taatacttag agaaggatat aaaaatttag atgatttacc tcatggagca
   134041 gtaataggaa caggaagtaa aagaaggaag tttcaattat tgaaacaaag acctgattta
   134101 aatatagtac aagttagagg taatgtggaa actaggatac gaaaaataaa agatgaaaac
   134161 atgcatggta ttgtgttggc agcatcagga ataattagag ctaatttgca agataaaata
   134221 agttcttatt tacctgttga tgttgttatt ccagcacctg cccaaggagc tttagctata
   134281 gaaattagaa gcaatgatag tgcgatagag ggaatagtaa attcactgaa ggatgagaat
   134341 acagaaatcc aaatattggc tgaaagaggt tttttagatg gtgtaaatgg aagttgtcat
   134401 attccaatgg cagcttattg tgaaataatc caagataaaa tacatcttac tggcttatat
   134461 ggagattcag aaggaaaaaa agtagtaatc aagtctatag atggagatat ttcttctcct
   134521 agagagttag gtttaaagtt agctaaactt gtattgaagg agtatgagaa ttatgaaggg
   134581 taaggtttac ttagtagggg caggaccagg tgattataaa ctcatgactt taaaaggttt
   134641 agagtgtata agaaaatcgg atgtaatagt atatgataga ttagcaaata gtagttattt
   134701 aagagaagct aagcctgatt gtgaacttat atatgtaggc aaggcatcaa gtaatcatat
   134761 tttgccacaa gaagatataa atagaattat agcagaaaaa gctaaagaag gaaaaattgt
   134821 aactcgtctt aaaggaggag acccttatgt atttggcaga gggggagaag aagcagagac
   134881 tttagtagat gaaggtatag aatttgaagt agttccaggt ataacttctg ctataggagg
   134941 tttatgttat gcgggtattc ctataacaca tagagaccat gcttcgtcat ttcatgtaat
   135001 aacaggtcat ttgaagggtg atgatagtgg agaactaaac tggaatgctc ttgcaaataa
   135061 caaaggtact cttgtgtttt tgatgggaat ttcaaaccta gagaaaataa gtgaaaactt
   135121 aatgaaagaa ggaaaagata aggatacacc tgttgctctt ataagttggg caactagata
   135181 taatcaaaga gttgttacat ctacacttga aaatgtttat gaaactgcta ttaaagaaga
   135241 agtaaaaccg ccaactctta tagtagttgg gagtgttgta ggacttagag aaaagttgaa
   135301 cttctttgaa agtaagcctt tatttggaaa aagtattgct gtaactaggt ctagaaatca
   135361 aaatagtgta ttggtagaaa aaataatgga tttaggtgga aatcctatag aaatacctac
   135421 tattaaggta gaaaaaatac aaaataatat taacttagaa aatgaaataa aaaatataaa
   135481 taagtataat tatctaatac taactagtaa aaatgccgta gaaatcttct ttgaaaagat
   135541 atatgaaatg aattttgatt taaggatatt atctaattta aaggtatgtg ctataggttc
   135601 tgctacatca aatgagttga aaaaaagagg aattatagcg gatatagtac caaaaaaatt
   135661 tgtagcagag tcactgtatg aagagttagc gcctatttta aatgatgacg ataatatact
   135721 tatacctaga gcagaaaatg ctagagatta tctggttgac aaacttaagg ctatttgtga
   135781 agtaacagaa gtacatatat atagaacagt aatagatgag gagaaaaaag gtgagatatt
   135841 agatatttta aattctaatg acatagatta tataacattt actagttcct caacagtaaa
   135901 taatcttgtt gaaattatag gaaaagaaaa tattaaaata ttagaaaaaa taaaaacact
   135961 atctataggt cctataacta cagagacaat aaatagtttg ggaataaaat tgtacaaaga
   136021 atctgctaca tctactatag attcgttagt agatttaatt ataaaaaatt aattttggag
   136081 gcttgaatat gttaagaaga ccaagaagac taagagcaag taaagcaatc agaaatttgg
   136141 taagagaaac taaggtgaat gtagaagact taatatatcc actgtttgta gtagaggggg
   136201 aaaatataaa agaagaaatt aaatctttac cagatgtcta tcatttatca atagatatgc
   136261 tagaagacga aataaaagaa ataagtgagt tgggaattga acatataatg ttatttggtg
   136321 ttccagatga aaaagatgag tgtgcttcac aagcttttaa tgataatgga atagttcaaa
   136381 aggcaattag aaaggtaaaa gaaataaatt cagatataaa tgtaataaca gatgtctgta
   136441 tgtgccaata tacaagtcat ggacattgtg ggattttaac tgatactggt tatgttgata
   136501 atgataaatc attggagtat ttggctaaaa tatctgtaag ccatgctaaa gcaggagctg
   136561 atatggttgc tccatctgat atgatggatg gaagaataca agcaataaga gaagctttag
   136621 atgaaaatgg attagaaaat gttggaatca tggcttatag tgttaagtat gcttcaaatt
   136681 attatggacc ttttagagag gcagctcatt cagcaccatc ttttggagat agaaaaacat
   136741 atcaaatgga cccagcaaat agcaatgaag ctataataga gactgaactt gatatattgg
   136801 aaggtgcaga tatacttatg gttaaacctg cattatcata tttagatatt ataagaagag
   136861 ttaaagataa ttttgactta cctctagcag cttataatgt aagtggagaa tactctatgc
   136921 ttaagtttgc tgtgaaggaa ggtttattaa atgaagatgt aattcttgaa tcaattacat
   136981 ctataaaaag agcaggagca gatataataa taacttattt tgctaaagac attgctaaac
   137041 tgttgaaaaa ataaattcta gcagtataaa gaatatcaaa aagtatagga attgtaatta
   137101 actattatac agtagttttg tttagacatt tattgaagaa taatagaaac taatttgcaa
   137161 tttctatact ttatttatgt aactaaataa aatttggagg tagaagatgc tatatcctat
   137221 taatttaaaa ttagatgagt tagatgtagt tataattggt ggtggagaag ttgcatatag
   137281 gaaatgtaaa aattttttag agtttaacaa aaatgttact atagtttcta aacaaatttt
   137341 aaataaattt tatgatttaa aaggaaatat aaaaataata aaagatgatt ataaagaaat
   137401 atatataaaa gatgcttctg taataattgc tgccacaaat aatagagagt taaatatgga
   137461 aataggatta tattgtaaag caaaaaataa gctagtgaat gtagttgata atatagaaat
   137521 atcgaacttt actgttccat catatataaa aagaggagat ttacttataa gtgtgtctac
   137581 tgggggcaag agtccttcat tatcatcaaa gataaaaaaa gaaattgaag agagatatac
   137641 tgaagattat gaggagtatt taaatgtatt aggagatata agaaaaaagg ttataaaaaa
   137701 atacgaagat aaaagtaaaa gaaaagatgt gcttaatatg ttggtaacac ttgacctaga
   137761 agaacttaag aagtttgata tataaaaaat aaaataaaaa atgttaaaaa ggtgttgacg
   137821 gattattttt aag
//
LOCUS       DAMJWU010000003       106777 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMJWU010000003 DAMJWU010000000
VERSION     DAMJWU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 106777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.003"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(120..1721)
                     /locus_tag="PC460_000251"
     CDS             complement(120..1721)
                     /locus_tag="PC460_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2599160.1"
                     /translation="MIYDLILKNGFIIGGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIAKQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1832..2509)
                     /locus_tag="PC460_000252"
     CDS             complement(1832..2509)
                     /locus_tag="PC460_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599161.1"
                     /translation="MSKVKSIYNEEYLPFKIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2522..3217)
                     /locus_tag="PC460_000253"
     CDS             complement(2522..3217)
                     /locus_tag="PC460_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2599162.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3269..4468)
                     /locus_tag="PC460_000254"
     CDS             complement(3269..4468)
                     /locus_tag="PC460_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2599163.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4729..6774)
                     /locus_tag="PC460_000255"
     CDS             complement(4729..6774)
                     /locus_tag="PC460_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2599164.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7185..7994)
                     /locus_tag="PC460_000256"
     CDS             complement(7185..7994)
                     /locus_tag="PC460_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2599165.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQKFLQFLEE"
     gene            complement(8078..8611)
                     /locus_tag="PC460_000257"
     CDS             complement(8078..8611)
                     /locus_tag="PC460_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2599166.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8761..10110)
                     /locus_tag="PC460_000258"
     CDS             complement(8761..10110)
                     /locus_tag="PC460_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2599167.1"
                     /translation="MELKNFKDILFKKALSEGFEEYEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(10122..11504)
                     /locus_tag="PC460_000259"
     CDS             complement(10122..11504)
                     /locus_tag="PC460_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2599168.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            11748..12962
                     /locus_tag="PC460_000260"
     CDS             11748..12962
                     /locus_tag="PC460_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2599169.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFNFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            13139..15052
                     /locus_tag="PC460_000261"
     CDS             13139..15052
                     /locus_tag="PC460_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2599170.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            15323..16297
                     /locus_tag="PC460_000262"
     CDS             15323..16297
                     /locus_tag="PC460_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2599171.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIIALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            16318..16593
                     /locus_tag="PC460_000263"
     CDS             16318..16593
                     /locus_tag="PC460_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2599172.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            16606..17769
                     /locus_tag="PC460_000264"
     CDS             16606..17769
                     /locus_tag="PC460_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2599173.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKTGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            18106..20385
                     /locus_tag="PC460_000265"
     CDS             18106..20385
                     /locus_tag="PC460_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2599174.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(20480..21820)
                     /locus_tag="PC460_000266"
     CDS             complement(20480..21820)
                     /locus_tag="PC460_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2599175.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(21832..22614)
                     /locus_tag="PC460_000267"
     CDS             complement(21832..22614)
                     /locus_tag="PC460_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599176.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(22629..23651)
                     /locus_tag="PC460_000268"
     CDS             complement(22629..23651)
                     /locus_tag="PC460_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2599177.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(23657..24670)
                     /locus_tag="PC460_000269"
     CDS             complement(23657..24670)
                     /locus_tag="PC460_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2599178.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTKFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(24798..25736)
                     /locus_tag="PC460_000270"
     CDS             complement(24798..25736)
                     /locus_tag="PC460_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2599179.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEDKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            26006..26470
                     /locus_tag="PC460_000271"
     CDS             26006..26470
                     /locus_tag="PC460_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599180.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILDGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(26582..26899)
                     /locus_tag="PC460_000272"
     CDS             complement(26582..26899)
                     /locus_tag="PC460_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2599181.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(26912..27643)
                     /gene="chbG"
                     /locus_tag="PC460_000273"
     CDS             complement(26912..27643)
                     /gene="chbG"
                     /locus_tag="PC460_000273"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF2599182.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(27669..28979)
                     /locus_tag="PC460_000274"
     CDS             complement(27669..28979)
                     /locus_tag="PC460_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2599183.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(29076..30389)
                     /locus_tag="PC460_000275"
     CDS             complement(29076..30389)
                     /locus_tag="PC460_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2599184.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(30411..30722)
                     /locus_tag="PC460_000276"
     CDS             complement(30411..30722)
                     /locus_tag="PC460_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2599185.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(30922..31626)
                     /locus_tag="PC460_000277"
     CDS             complement(30922..31626)
                     /locus_tag="PC460_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2599186.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCEYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(31717..33078)
                     /locus_tag="PC460_000278"
     CDS             complement(31717..33078)
                     /locus_tag="PC460_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2599187.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            33577..34731
                     /locus_tag="PC460_000279"
     CDS             33577..34731
                     /locus_tag="PC460_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2599188.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(34813..36531)
                     /locus_tag="PC460_000280"
     CDS             complement(34813..36531)
                     /locus_tag="PC460_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2599189.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLFTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(36536..37525)
                     /locus_tag="PC460_000281"
     CDS             complement(36536..37525)
                     /locus_tag="PC460_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2599190.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(37621..38670)
                     /locus_tag="PC460_000282"
     CDS             complement(37621..38670)
                     /locus_tag="PC460_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2599191.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(38947..39615)
                     /locus_tag="PC460_000283"
     CDS             complement(38947..39615)
                     /locus_tag="PC460_000283"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF2599192.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(39621..40994)
                     /locus_tag="PC460_000284"
     CDS             complement(39621..40994)
                     /locus_tag="PC460_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF2599193.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(40991..42769)
                     /locus_tag="PC460_000285"
     CDS             complement(40991..42769)
                     /locus_tag="PC460_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF2599194.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(42886..43209)
                     /locus_tag="PC460_000286"
     CDS             complement(42886..43209)
                     /locus_tag="PC460_000286"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF2599195.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(43196..44173)
                     /locus_tag="PC460_000287"
     CDS             complement(43196..44173)
                     /locus_tag="PC460_000287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF2599196.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(44188..44751)
                     /locus_tag="PC460_000288"
     CDS             complement(44188..44751)
                     /locus_tag="PC460_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF2599197.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(44803..45294)
                     /locus_tag="PC460_000289"
     CDS             complement(44803..45294)
                     /locus_tag="PC460_000289"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF2599198.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(45297..47222)
                     /locus_tag="PC460_000290"
     CDS             complement(45297..47222)
                     /locus_tag="PC460_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF2599199.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVIFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(47224..47535)
                     /locus_tag="PC460_000291"
     CDS             complement(47224..47535)
                     /locus_tag="PC460_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599200.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(48165..48440)
                     /locus_tag="PC460_000292"
     CDS             complement(48165..48440)
                     /locus_tag="PC460_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF2599201.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(48555..49964)
                     /locus_tag="PC460_000293"
     CDS             complement(48555..49964)
                     /locus_tag="PC460_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF2599202.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(50335..50997)
                     /locus_tag="PC460_000294"
     CDS             complement(50335..50997)
                     /locus_tag="PC460_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF2599203.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(51251..53470)
                     /locus_tag="PC460_000295"
     CDS             complement(51251..53470)
                     /locus_tag="PC460_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2599204.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFVAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(53746..56400)
                     /gene="adhE"
                     /locus_tag="PC460_000296"
                     /gene_synonym="adhC"
     CDS             complement(53746..56400)
                     /gene="adhE"
                     /locus_tag="PC460_000296"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2599205.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            56904..57104
                     /locus_tag="PC460_000297"
     CDS             56904..57104
                     /locus_tag="PC460_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599206.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(57311..57901)
                     /locus_tag="PC460_000298"
     CDS             complement(57311..57901)
                     /locus_tag="PC460_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF2599207.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(57904..58779)
                     /gene="dpsA"
                     /locus_tag="PC460_000299"
     CDS             complement(57904..58779)
                     /gene="dpsA"
                     /locus_tag="PC460_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF2599208.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(58855..60246)
                     /locus_tag="PC460_000300"
     CDS             complement(58855..60246)
                     /locus_tag="PC460_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2599209.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRIIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(60250..61386)
                     /locus_tag="PC460_000301"
     CDS             complement(60250..61386)
                     /locus_tag="PC460_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF2599210.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(61418..61963)
                     /locus_tag="PC460_000302"
     CDS             complement(61418..61963)
                     /locus_tag="PC460_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF2599211.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            62287..63066
                     /locus_tag="PC460_000303"
     CDS             62287..63066
                     /locus_tag="PC460_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599212.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            complement(63521..63949)
                     /locus_tag="PC460_000304"
     CDS             complement(63521..63949)
                     /locus_tag="PC460_000304"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2599213.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     repeat_region   64010..65541
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=64205..64233
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(65725..65991)
                     /gene="cas2"
                     /locus_tag="PC460_000305"
     CDS             complement(65725..65991)
                     /gene="cas2"
                     /locus_tag="PC460_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2599214.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     gene            complement(65994..66974)
                     /gene="cas1b"
                     /locus_tag="PC460_000306"
     CDS             complement(65994..66974)
                     /gene="cas1b"
                     /locus_tag="PC460_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2599215.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            complement(66977..67528)
                     /gene="cas4"
                     /locus_tag="PC460_000307"
     CDS             complement(66977..67528)
                     /gene="cas4"
                     /locus_tag="PC460_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2599216.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            complement(67543..69864)
                     /locus_tag="PC460_000308"
     CDS             complement(67543..69864)
                     /locus_tag="PC460_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2599217.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTYLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            complement(69875..70576)
                     /gene="cas5b"
                     /locus_tag="PC460_000309"
     CDS             complement(69875..70576)
                     /gene="cas5b"
                     /locus_tag="PC460_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2599218.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            complement(70578..71492)
                     /gene="cas7i"
                     /locus_tag="PC460_000310"
     CDS             complement(70578..71492)
                     /gene="cas7i"
                     /locus_tag="PC460_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2599219.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            complement(71494..73344)
                     /gene="cas8a1"
                     /locus_tag="PC460_000311"
     CDS             complement(71494..73344)
                     /gene="cas8a1"
                     /locus_tag="PC460_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HDF2599220.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNMIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKTPPEEILQIFVEEDLDFESVGYAFVTGLISGKYEGKDKLPNEKEEK"
     gene            complement(73350..74087)
                     /gene="cas6"
                     /locus_tag="PC460_000312"
     CDS             complement(73350..74087)
                     /gene="cas6"
                     /locus_tag="PC460_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2599221.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            complement(74165..75082)
                     /locus_tag="PC460_000313"
     CDS             complement(74165..75082)
                     /locus_tag="PC460_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2599222.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            complement(75361..77277)
                     /locus_tag="PC460_000314"
     CDS             complement(75361..77277)
                     /locus_tag="PC460_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2599223.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            complement(77277..78023)
                     /locus_tag="PC460_000315"
     CDS             complement(77277..78023)
                     /locus_tag="PC460_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599224.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            complement(78127..78978)
                     /locus_tag="PC460_000316"
     CDS             complement(78127..78978)
                     /locus_tag="PC460_000316"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2599225.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            complement(79226..80230)
                     /locus_tag="PC460_000317"
     CDS             complement(79226..80230)
                     /locus_tag="PC460_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2599226.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFAP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKGVNSLIKM
                     "
     gene            complement(80240..80932)
                     /locus_tag="PC460_000318"
     CDS             complement(80240..80932)
                     /locus_tag="PC460_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2599227.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            complement(81246..82205)
                     /locus_tag="PC460_000319"
     CDS             complement(81246..82205)
                     /locus_tag="PC460_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2599228.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            complement(82238..83116)
                     /locus_tag="PC460_000320"
     CDS             complement(82238..83116)
                     /locus_tag="PC460_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599229.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIRSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            complement(83109..83891)
                     /locus_tag="PC460_000321"
     CDS             complement(83109..83891)
                     /locus_tag="PC460_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2599230.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            complement(83892..84521)
                     /locus_tag="PC460_000322"
     CDS             complement(83892..84521)
                     /locus_tag="PC460_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599231.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            complement(84916..85542)
                     /locus_tag="PC460_000323"
     CDS             complement(84916..85542)
                     /locus_tag="PC460_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2599232.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            complement(85618..86589)
                     /gene="nrdF"
                     /locus_tag="PC460_000324"
     CDS             complement(85618..86589)
                     /gene="nrdF"
                     /locus_tag="PC460_000324"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF2599233.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            complement(86601..88700)
                     /gene="nrdE"
                     /locus_tag="PC460_000325"
     CDS             complement(86601..88700)
                     /gene="nrdE"
                     /locus_tag="PC460_000325"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF2599234.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            89178..89903
                     /locus_tag="PC460_000326"
     CDS             89178..89903
                     /locus_tag="PC460_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2599235.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(90031..91227)
                     /locus_tag="PC460_000327"
     CDS             complement(90031..91227)
                     /locus_tag="PC460_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599236.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFNLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(91230..92255)
                     /locus_tag="PC460_000328"
     CDS             complement(91230..92255)
                     /locus_tag="PC460_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2599237.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(92327..93457)
                     /locus_tag="PC460_000329"
     CDS             complement(92327..93457)
                     /locus_tag="PC460_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2599238.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(93685..93866)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(93919..94812)
                     /locus_tag="PC460_000330"
     CDS             complement(93919..94812)
                     /locus_tag="PC460_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF2599239.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(94892..95794)
                     /locus_tag="PC460_000331"
     CDS             complement(94892..95794)
                     /locus_tag="PC460_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2599240.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(95844..97004)
                     /locus_tag="PC460_000332"
     CDS             complement(95844..97004)
                     /locus_tag="PC460_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2599241.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(97160..97465)
                     /locus_tag="PC460_000333"
     CDS             complement(97160..97465)
                     /locus_tag="PC460_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2599242.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(97563..98537)
                     /locus_tag="PC460_000334"
     CDS             complement(97563..98537)
                     /locus_tag="PC460_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2599243.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            99119..99895
                     /locus_tag="PC460_000335"
     CDS             99119..99895
                     /locus_tag="PC460_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2599244.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            100228..101373
                     /locus_tag="PC460_000336"
     CDS             100228..101373
                     /locus_tag="PC460_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2599245.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(101528..102397)
                     /locus_tag="PC460_000337"
     CDS             complement(101528..102397)
                     /locus_tag="PC460_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2599246.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKELVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(102515..103228)
                     /locus_tag="PC460_000338"
     CDS             complement(102515..103228)
                     /locus_tag="PC460_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF2599247.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            complement(103432..104820)
                     /locus_tag="PC460_000339"
     CDS             complement(103432..104820)
                     /locus_tag="PC460_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2599248.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            complement(104941..106218)
                     /locus_tag="PC460_000340"
     CDS             complement(104941..106218)
                     /locus_tag="PC460_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF2599249.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            complement(106355..106690)
                     /locus_tag="PC460_000341"
     CDS             complement(106355..106690)
                     /locus_tag="PC460_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599250.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
BASE COUNT        35524 a        18446 c        11890 g        40917 t
ORIGIN      
        1 aataaatatt aatactataa ataaaaacga agcatgattt ttatttaaat aaatctcaaa
       61 cttcgtcttt acttacttag atatttattt actttctaaa tcctattatt tttaaaacct
      121 tatcttttta atacttcacc acatttattc tcagtaatct ctccattttc caacgcaatt
      181 tttccattta caattacata ttcaacacca ttggctagta attgtggctt tgaaaaatca
      241 atatcttctg acctagaata actatagtta tccatgtcaa taactgctat atcagctatc
      301 aacccttctt taagaatacc tctatttttt attttaaatt gcctagctgg taatgaagta
      361 actcttctta caatctcttc caatctaaca ccagactttc tgtacatatt gaagaaaatt
      421 ggaaaagctc ctcttctttg tagttcaaac catgacatat ccccacccac actaaacaaa
      481 caatctgaac caaccataac aaatggatta tcaattattt tatcatcatg tagtttatct
      541 gggaaatcag ctctatacat tcctcctaaa caaaagataa gttcttcgtc tccatctaaa
      601 agtaaatcga gtaataactt gtcttcatct attccttttt gtgtagctat ttcacgtatt
      661 gatttctttt ccatattaat atctttagtg tttagaagta ttatactccc cctatctcca
      721 agtataaatg cttcttttaa tataagtgtt cttccctctt catttctaag agcatccatt
      781 accccattta tacctaattc aaatagacta gatgatattg ccataataaa ttgaattact
      841 ctttttttct tcatacaatg cccagaactt cttggtactg tgtcaaccat tatttcaaca
      901 ccattatacc tagcctttaa tatctcatca aatgcttcta tttcaactgg acttaagtga
      961 gatacttgaa ccctagcacc acttttttgc ccaacctcta tggcttcttt tactgcatta
     1021 tatcttccta tgtaatctct aatatgagat gtatatatac catcatgctc tttaatctgc
     1081 tttgcaatat caacaagttc atcaatatca gcatacttac ttggtatata tgccaatcca
     1141 gtagatattc caaaagcacc acttttcatt ccatcattta ttatatctgt aatttcattt
     1201 ttttcctttt ctgtaggagg tctatcctta gaacctccca taacactcca tcttatagtc
     1261 ccatgcccca acaagaatcc catattgaca gaaataccac ttttagatat aaggtcacaa
     1321 tattctgtaa aattattcca acatttattt ttatctataa gatacttttt ttcttcttca
     1381 agtaatagac catttttata catatattca tatacatttt cagaagaata tggtgttata
     1441 gaatgtccac aattaccatt tatagttgtt gtaactcctt gttttagaaa cttttcaaat
     1501 ttaccatcta gtattgctac tatttcttca tgaacatgag ggtcaataaa tccaggtgtt
     1561 ataacttttc cacaacaatc aatctcttta tttgtattgc tatttatctt agaatctatt
     1621 tttgctatta agttgtcttt aattgctata tctccataaa aaccaggatt acctgtacca
     1681 cctattataa atccattttt taagattaaa tcatatatca aaatattccc tccttaaaaa
     1741 ccaattttta aataatctta atgtaatttt tatatttgaa aatcaaatta ttatatttca
     1801 tatttgaaaa ttcactatta tttcattgta attagtcact aagcttatct ttatacatag
     1861 ctcttgcaat taatatagta ccaaatacac atataagaac tactagagac tgtggtatta
     1921 taccttgctt tgataatata atagtcaata cagatattac aattgctact aggcctaatt
     1981 ttttatcttg taaaaatact tgtccaaaaa ctgacccaaa tagagctggt aaaattaagt
     2041 ttaatttttc tactacttca gctggaattt tagatagtac gcttcctcct aaaattaccc
     2101 ctatagttag tatacttata ttcactaaaa tagatacagc tattcctatt gtagatatta
     2161 tcgagccttc ttctgtacct gattcaactt ctgctgcctt ttgagctgct attgaacatg
     2221 gtagcctcat attagatata ttaccagaaa gaaaactcat ataagttcct gggataccta
     2281 agattggtga ataagaaact ggttctatta tgtaatttgg agcactcata ctgacaattg
     2341 ccattgtact tgctaataat gctgatattg gaggcataat accaaatcca aagctcaaaa
     2401 ttattccagg aactaatgct gctataatcc ccaaagatag tgttagtctt ccatacctta
     2461 tcttaaaagg taaatattct tcattgtata tagattttac tttactcata aaactccccc
     2521 cttatattac tgaagctatt atcattccgc taaacattga tattgtaagc ccccactctc
     2581 ttaaccattt tatatttttc ttcttttcaa gcatacacaa tattaccatt atagccattc
     2641 cacttataca tgcaattgtt ccattatcaa aacgcaacac cctatcagca tttagatatg
     2701 caaatgaacc caacattgct cctagagata ttattggtat aaatgatttc ttgccactag
     2761 atataacatt ggttatctta tctagtttat gtccaaatag taatgtgaat attatccatc
     2821 ctaaagaacc taatatcata gtccaaactg catttgtaaa tgcaagtttt gtcatttcag
     2881 gagaacctaa agttatacct aatgcatctg ttccaaatcc ggctgacatt gattcaaaaa
     2941 ttacagaacc tataaatgat aatctcatcc atgctatagg cccacccata gttactataa
     3001 gcgataccat tccagcaaat atagtcattg aaggcccaat agctgaaata gcactactct
     3061 taacagctga ctttatttgt ttatctgtta ttcccattgt tttaccaact ttgcaagatt
     3121 ttatagcaaa tataagggat tgtaccacta ctattgatac tgctactgta ctcgctatcc
     3181 acaaaagtgg atgattagct aattttaaat aatccattta taaaacctcc ctataaatcc
     3241 tcaaatttta ttaatatttc taatatatct atttccaaac ctgttttagt actctaagta
     3301 tatttccacc tagtaccttc tctatttcct catcactata tccatgcttt actaaatatc
     3361 taacaatgtt ttttgaagtt tctgttgggt tctcaagacc ttttacatat tctactttgg
     3421 gatgttcttt tgttcctgtt atttttttta tatcaaattt agcagctagt actgagtgta
     3481 attttacatg gtcgccatat actgtatctg gtccaaaagc tacatggtct attcctacta
     3541 aatttttaat atactcaaaa tgttccataa atgactctat tgaatgttct ttattcttct
     3601 ctgttattgt agtatgaggt gctgcttcta cacctataac ccctcctttt ttagcacatg
     3661 ctattaatac ttcatctgga gttagtctct ttgtgttcca taaacttctt gccccaacat
     3721 ggcttaatat tattgggtcc ttgctatatt ctattacatc aagtgtagtt tgaactccaa
     3781 cgtgagagca atctattgcc attccaattt tattcattct ttcaacagct ttttttccga
     3841 aatttgtaag acctccatct ttctcttctt ttagtcctga acctaatgca ttggattcgc
     3901 tatatgttat acccattaac cttataccaa atccatacag tatatctatt ctatcaagtt
     3961 cattttctat tggtgctgca ccttctaatg ttggtatcaa tgctacttta ccttcacttt
     4021 tagctctgta tatatcttct actttttcgc actttataac aaaatcttga tgagctaaat
     4081 cacatagtct catgcctaaa tctattaata tatcatccca tttccaccct gaatttgatg
     4141 ttatcgtaca tacaccatcc attaaattat caaatatagc atcataatat cctcttgata
     4201 atgcttcata agcacatctt tgtttacctt ccctattata ttcaaatata tctctttcta
     4261 ggtgttctgg aaataatact ggatgttcat gtaatgatat aaatattttt tcatccacta
     4321 tacttttaac cttagcttct tgaatatcat ctaattcaat aaggtattct ttaactcttt
     4381 tatctccttt acacatctca atagatgaaa tatcttttcc tttttctaaa taactatagg
     4441 cttcatatcc tttgtaagct gacttcataa caacatcctc caagttctac aattttttat
     4501 atttttccta tgtagcaatt gatgtgccaa aatataaatt ttaattgtat aataaatctt
     4561 gcaaaaaata aaaacagtag taaggttgat atttactact gttttattaa aaataatctt
     4621 cttagattat ttattcaata tttttgaatt atcttaatct taaagataat tttatttatt
     4681 tctatccatt atagggattt ttttagggtg ttttgccttt ttagtcccct atatcactcc
     4741 cttaattttt acatatgata taaagttctt tccttcatca gttataaaac taccttgttt
     4801 ttttactcca gataaaagta aaccatggct ctttaactta tctattcttt ttctaatttg
     4861 gtctacactt aaaactatat ctttttcttt taaaatctct tgcagtttat ttcttcctaa
     4921 taatatattt ttattattcc aagtttctat actggtcaat atacatatac tttcctcaaa
     4981 gaaattagat tgtttgaaat ctaagataat tgaatcgaaa ttttcattaa ctaaagtatt
     5041 gtttttttca aatctaaatt gttctggcaa atgctcgtaa tctactctat cttcctcaac
     5101 tatagtatca atataataaa ttaaattttt aagctctctt acatttcctg gccattcata
     5161 caatgttaaa gcttgcattg attcctctgt aaaacatttg ttgctattta tttcatctaa
     5221 aaaatactta cttataagag gtatatcttc cttacgttct ctaagtctag gtatctctat
     5281 atgtaataca ttaatcctat aatacaaatc ttctctaaaa gaaccttctt taattttttt
     5341 ctttaaatct ttatttgttg cagctataat tctaacatct attggtataa ttttacttcc
     5401 gcccattctc ataacttctt tttcctgtaa tactcttagc aatctctgct gtacatctaa
     5461 agatatatca cctatctcat ctaaaaaaat tgttcctgta tgtgctcttt caaatatacc
     5521 catttttcca ccttttattg ctcctgtaaa acttccttca tcatagccaa aaagttcact
     5581 ttcaataagt gtatctgaca aagaagatag attaactgca acaaatggct tgttttttct
     5641 tagagattca ttatgtattg cctgggcaaa tatttctttt cctgttccat tttctccaag
     5701 tatcaacaca gaaaagtctg tagttgctat ttttcttgca atattaattt tttcttttat
     5761 tattttactc tcacctacaa cactctcaaa tgtatacttt gatacaaaac cttttgactg
     5821 aagtttattt tgaatcttgt cctcaagcac ttgaattgca cttatatttt taatgcttat
     5881 aatactcttt attcgttcat tattttcata aacatttact ttattaatta tcagtttttt
     5941 gttatttaaa tttactactt catcattaac ttcatcttct tgaaaaaata tttttttgac
     6001 aaccttatca ctaaataagt ccataaagtt attagatata atttcatttt ggtctattcc
     6061 aactagatta gaaaatactt tgttgcaata tataaatttt cctaatttat caacagaagc
     6121 aataccttca tctataatct ccaaaaaact cttcatggtt ttattcatag ttgtataata
     6181 cctatatcca cttactgtat cttcattgta tttttcctta ataatatgta gtttatctat
     6241 agatatattt agttttgtaa agatttctat aactgtattt atatcaatta ccttatcacc
     6301 tatgtcaatt atttgcttta tgttttgtgg tatgctgttt ctgcttcccg taataattcc
     6361 aatctcacat ttagatttat cacaacctgg ataatatggt attagattaa tatgaattaa
     6421 tcctagtttt ctaattatct tggcagtttc atctgctgat tctttataag catctataac
     6481 cataacatca gaatcatttt ctatacttat tatctgattt atattttcaa tatttatagt
     6541 acgatgtact attacaatag gtatattttt atcaatatta ttatgtatat attcctttat
     6601 atcattgtct gaacatacta caagctcata gcttgacagt tcaatattta tcctatttac
     6661 atataccaag ttatctatat tacaaaattc tccaaatata ttttccaatt gttttgatat
     6721 atctatattt attttgtcat cagttgatac aaataaaata ttatgttttt tcatagtccc
     6781 acctcctgtt taacgagtat agaaagtaat aatttggtct attgtagcac tttttaaaaa
     6841 agttttaaat cttaaaaatc aaattttgta ccatttacta ttattttcca taataaaata
     6901 atgtaagcta tcttttttca catataattt aatttctaaa acggttagtt tgacattatt
     6961 ggaatataaa caaaaattta atataaattc gttaatatat taatacattt ttaagaatga
     7021 caaggaataa attataacaa attttaggaa tatataataa tagggtctca gaacttagtt
     7081 acaagttcta aaaccctatt tgtttagtgt aaaaaacatc ataaaattta gcttaaatct
     7141 tttcacataa tattactctt tttctaagtg ataataaaat ttatctattc ctctaaaaac
     7201 tgtaaaaatt tctgattaaa tagtttaagc tcatcataga atatagcatg accactatac
     7261 tcaaaagtat ataaaattga atcttctatt ttttcattca taaaaactgc aaattcatac
     7321 ggacatattt catctaattt cccatgaaat ataccagtag gcactcttac acattttaaa
     7381 tcattaaata attcttcatc tcttaaagat acagcagttc caatagtgcc aatacctgat
     7441 gcactaaaac caatatcatt aaaccatctt ctaaagcttt ctggtggatt tgatgcaaat
     7501 actttttcgc caaaatcggt taccatttct ggtctatcag tacatgcttg tgctatcaat
     7561 ttatttacat cttcacgtgt cataccatat ggaaattctg gaggtctttg tacaaatgat
     7621 ggtgcagcag ctgcagctaa taccaatttt gacactccat atccattaaa taaactcata
     7681 tacctaagta cgattgctcc tcccattgaa aaaccaacaa gggtaaagtc ttttaatcca
     7741 atagcatgta ccaccttata aatatcgtct gctaattggc tataagtata tcctcctgat
     7801 gttgcatcag atttaccaaa acctcttaaa tcaattgaaa ctgttctata tccgagtttt
     7861 ggcaatatat ttgtttgata ttcaaatatc ttatgtccta agggccaacc atgtataaaa
     7921 agtacagttt ttttagcact cggattcaaa tcatatactg ctatgtttat atcattcaca
     7981 tatacataat acatatcaac agcaactccc ttcaagaaaa tgtatgatgg ttattttata
     8041 tatatgaata aaatgatgaa taactaaatt ttcaaattta tttacaatac ttctctattg
     8101 ccttttcaaa taaacttaca tgaagttttt catctaggat tattctattt aatatagctt
     8161 ttatataagg gtcttcaata agacttatat ctttataata attattaata gccacatgct
     8221 ccaaatgcaa atctagtttt aatctgttac aaatagaatt tccataacat acaaaaccac
     8281 cattccaata tgcaccacaa gaacttgtag aacctctata tacaggattt ccacccaata
     8341 atcttatagt ctttgctaat atctccatat gtaacatttc tgttatagat atatttatcc
     8401 acatttcact taactctcta tgcgtttcat ctaaatcaaa attatggtat agatattgat
     8461 ttacactcgt aaattcactt gatactccgg catagtcatc catcaataat tctgcataat
     8521 acttatttgg acctaaaact tttatctctg gatatggctc atcacttgaa tatccatttc
     8581 gcttgtgcat gtcataatgt gcatcactca taatatctcc tcctttaaat ttaatacatg
     8641 atattaattg tatgcatcac gactttttca tataacttag ataataaaat ctacttaaat
     8701 aataaaaagc catattaatt aaaattaaca tgacctttta tccacaaaat tactttttaa
     8761 ttattcatct tttccagcta ctgatagctc tttaataaca actgaaggtg aacctatact
     8821 actcattgga aattctaaat cttcccctat agcttcaata tctttcaaca ggtcaaaata
     8881 gtttcctgct actgtaattt gctctactgg aaatactttt tttccttctt tgatataaaa
     8941 gcctttggca gctaaagaaa agtctcctgt tactgaattt gcacctgaat gaagtcctgc
     9001 aaagctagta accatcaatc cttcatctat atcctttata atttcatcta aagatttatc
     9061 tcctttctct atataaaaat tagttggata tatgcttata ggtgatgaat atgaagattt
     9121 tattccattt cctgtagtct ttacacctgc cttattagcc gtttttaagt tatgaagtaa
     9181 agttataagc tttccattta aaacaacttc ttttttaaat gtagctactc cttcatcatc
     9241 aaaaggagta gatgccatac cattttctaa aagtgggtca tcaactattg taactatagg
     9301 agatgctatc atgtctcctt ctctattttt caataaagat aatccttttt gagctgaatc
     9361 tgcattaaat atcccagaaa atgtgctaag caaagatacc atagcttcat taagcaatat
     9421 agttttatat ttacctgatg gaatactctt accacctact ttagataaag cattatcaac
     9481 accaagtttt gctatctcac aaggttttat ttcttcaata gaatttgcta tattatatcc
     9541 tattccatca tatttttgtc cattgtcttc tatgatagga actacaaacc ctataagcat
     9601 attagtttta ttgcttaaat taagaccttt agtattagat ataccattac ttgatacaga
     9661 ataagatata gtacatttag ataaatttac caccttatct gaataagctt tagtctctct
     9721 ttccatttct aaagctaaat caatcaactt atctgcctct aaattttcta gctctttaga
     9781 ataagtcttt acatcattat agtgcttatc tccttcatat ataaattgta catcttcatt
     9841 ttcaatagtg tttactccat cttttacatt ttttatcaac atatcaacag ctttatcatc
     9901 taaaatttct gtataagaat aacctatctt tccatttacc ttacctctaa aagataaacc
     9961 aaaagattta tcaagattgt atttctcaac ttctccttca tatatattta tacttaaatt
    10021 ttctcctgta taataatata tttcatactc ttcaaaacct tcacttagag cctttttaaa
    10081 taatatatct ttaaaatttt taagttccac aattatccct cctatcttcc acctacagta
    10141 atttctttta cccttatcat aggttggcct acattagttg gtatacttcc tgatgatgag
    10201 ccacacatcc cctgagcttg ttttacgtta ttcccaacca tatctatatt cataagtata
    10261 tcgctacctt ttccaattaa actagcacct ctaactggct cttgtatttc tccatttttt
    10321 attaagtaac cttctgaaac tgcaaaatta aactctcctg ttacaggatt tacagaacca
    10381 ccacccattt ttttagcata aagtccatct ggtattgact ttataatatc ttctggtcta
    10441 tcttctccag cagcaatata agtgttagtc atccttgaag ttggtgcaaa tttgtagttc
    10501 tgtctacgag aacttcctgt aggattcatt cccattcgtc taccattaag tttatctatc
    10561 atataagact tcaaaattcc attttcaatc agtatattct tttgagttgg atttccttca
    10621 tcatctatat tcatagaacc ccagtagttt ggtattgtac catcatctat agctgtaatc
    10681 tttgtggaag caatctgttg acctaattta tctgcaaata cagagttccc ctttgcaact
    10741 gatgtagctt ccaaagaatg tccacaagct tcatgaaata ttactccacc aaatccatta
    10801 tctattgcta ccatcatatt acctgcagga caatttttag catgcaacat agtatgcgct
    10861 actctagctg attcttttgc ataatattct gggtctattt cttcaaatat ttcaaaccct
    10921 ttacaacccc caggaccttc aaatccagtt tgattttcat tacccttaga tgcaattgaa
    10981 cttatcccta gtctggttct tattcttcta tcctcaacat atataccttc agtgtttgca
    11041 attaatattt tttggtcttt atccaaatag ctaacactta cttgagatat atcattacta
    11101 aattcttttg cactcttata tgcaaccttc ataacactta ctttatcttt atttcctatt
    11161 gaatttggat aaatttttat attatgtatg ttatttattt taatactgtc atttaatatt
    11221 acactttttc catcttccaa tttacctaaa gcaacagcag ctttgtatgc tacatccaac
    11281 agactattta atgaattgtc acttgtataa gcatatatac ttttcaatcc tttaaatatt
    11341 cttattccta taccataatt tcttccacct attgcatttt ctactttatt atctattaaa
    11401 ctaatagaat tatttatagt gtcttcttca aaaatctcag caaaatctcc tccagtaatt
    11461 agacattttt ctaatacctt agaagctata ttttttgaaa gcataaattt acctcctact
    11521 ttaaattttt aattaacttt tattcaatct ttttacttgt taacaaacat tatatactat
    11581 attacttaaa ataaggtaat tatgaatata ttatacatat gttttatact aaaatgttat
    11641 cacattttat ctatacatac ccatataact agataattaa aatgtataat attatttgct
    11701 atagaaaata atacactcat acataataaa ttttaggagt gaagtatatg aatcaagaat
    11761 atgtaaaagg aaatcctatt tttaccaata ttaataaagt tccaagacaa tttccatacc
    11821 ttactgatga tattgatact gatgttataa ttgttggtgg tggcgtaact ggatgtattt
    11881 gtgcctacta tctagctaaa aacaatataa agtctgtcat tcttgaaaaa ggaaggattg
    11941 cacatggaag cactagcgtg acaacttctc tattacagta tgaattagat gacaatttga
    12001 tagatttaac agaggttatg actctaaaag atgctctaaa agcatataac ctttgtactt
    12061 cagcacttga agaattagat acatttatag agctatatgg aaataagtgt gattatgcca
    12121 aaagagatac tctactttat actgccaaca aacttgaagt taaggctatc aaagaagaat
    12181 ataacttaag aaaagaaaat ggatttgatg ttgaatatat tgatgaatcc acaaaccctt
    12241 ttaactttga tttaaaatca ggattgatag caaagaatgg aggacgagaa ttagacccat
    12301 ataaatatag tcaccatttg attgatgtaa gtttaaaaaa tggtcttcaa gtatatgaaa
    12361 atacagaagt aaaaaaagta gatttatcaa atgataaggt tactgctgaa gtatcgtatg
    12421 gatacaaagt tcatggtaaa aaattaattg tagctacagg gtacaataca agtttattta
    12481 caaaaagaaa ttttgcaact aagtcaaata cttttaatat agcaacgaaa ccacttaaaa
    12541 acatagcttc ttggaaaaac aacattttaa ttagagataa ctgtgaccca tataattatc
    12601 taagaactac taaagacaat cgattaatta taggtggtga agatgtaagt tttgatgata
    12661 tagaaaatga aactttagcc aatgaaaagt atgatatttt agaacaaaga ttgaaaagta
    12721 tgtttaagga tataaaagac attgaagttg aatataaata ttgtggttgt tttgcttcaa
    12781 ctttagataa tttaggtttt attggtccag ataataaaaa taacaatcta tggtattgct
    12841 taggttatgg agcaaatggt atactctttg caatattagg aggtataatg ttaagtgaat
    12901 tatatcttgg caaacaaaat aaaaatatga aattatttaa agtagataga tttgataaat
    12961 agatattata tattaaaaga ctgtaaaaag atgaaacttt ataattctaa aagttaactt
    13021 tgatacagtc ttttataatg ttccttttaa aaatacttta tttataatta aatataaagt
    13081 aaaatgttaa aatataactg ataaaattta gtcataagat aatatttaag tttatttgat
    13141 gaaatatgga ggaattatta tgaaggaaat actcgttatt gctcccacta aaggaaccta
    13201 tgaaaaatca attcacatag ttaaaaaaaa caaatataca aatattgatg ttgtatttgg
    13261 caatctaaaa gaaggtatac ctcttgctga aaaaagtatt aatcatggta ctagaattat
    13321 cataagccga ggtggaactt acaatatgtt aaaagctact tacaatatac ctatagttga
    13381 aattaaggta gatgcttatg atattataaa aagttacaaa gaggtaaaaa attccaacga
    13441 accatttgga ataattggat ttaataatgt catctacggt ttcgatatta tagaagaaat
    13501 attgaataag aaaataacta tgattgaaat tgaaaaagaa gaagaaattt atgatgctat
    13561 agaaaagtac agaaagaaag gtataaacac atatatagga gataccactg ttgcacatat
    13621 tgttaagaga cttaattgta agggaatatt aatcgaatct agagaagaaa acatacttag
    13681 agccattcaa caagcagaac aaatactcga agcaaccaag gatgaacaaa aaagaagatt
    13741 acagatagaa gctatgacag attttgtaca tgatggaata ataacagttg ataaaaattt
    13801 taaaataaca ttatttaata agagtgctga aaaaatattt ggtataaaaa aagaaaatgc
    13861 actttatcat aatgttgtag atgttatccc aaacacagtt cttccacaag tagttgagac
    13921 tatggaacct caaattggtg aaatacaaaa tatagggagt acaaaaatta ttacaaatag
    13981 agttccaatc attgttgatg gtcagattca aggggctgtt tcaacatttc aagatagcaa
    14041 ggagataagt tcttttgaac atacaataag aaggagctta ttaagcaaag gtctatctgc
    14101 taaatacact tttgatgata taattcatgc tagtgaaaag acaaaagatt gtatagaact
    14161 tgcaaaaaaa tatgcttgtt atgatactcc tatgattatt actggagaat ctggagttgg
    14221 taaagaacta ttttgtcaaa gtatacataa ctatagtaag cgtaaaaatg caccttttgt
    14281 tgctgtaaat tgtgctgcca tacctccatc tctaatagag agtgagtttt ttggttatga
    14341 ggaaggttca tttacaggtg ctagaaaaaa aggtaaagca ggtgtattcg aactagcaca
    14401 tgaaggtaca atcttcctag atgaaataag tgaaatccct cttgaacttc aaggtcgatt
    14461 attgagggta ttgcaggaaa aacaagttat gagaattggt ggtgataaag ttattcctat
    14521 agatgtaaaa attatctgtg cttcaaataa agacttaaaa aatatgatgc gcaatggttt
    14581 atttagacaa gacctttatt ttagaataaa tatactaaca ttatatcttc cttcattaaa
    14641 agaaagaaaa gaagatatta taaaattatc agaatttttt atacataaat attcaagcaa
    14701 atacaataaa acgcctttaa ttatcactcc aaatattaaa aaggcacttc ttgaacgaga
    14761 atatgaaggt aatataaggg aattggaagg tatgatggaa agggctgtta ttgtggaatc
    14821 atttgactcg attttattag aaaaggttag caatgaatat acaaataata tagttgataa
    14881 agataatagt gattttgaat gcagtaattt agatttaaaa actttagaaa aaaaatatat
    14941 ctataaaatt tataacgaaa ataatagaaa tactacaaaa acctgtgaaa ttctaaagat
    15001 aactcgctcc actctatggc gtaagttaaa agaaatagaa gatgatgttt aaaaataata
    15061 catacctata tatatcaaaa cgttttatgt ttaattttaa tacataagac gtttatttgt
    15121 tttcaataaa aaatacaatt atatgataaa aagaatcttt gtaagtgaaa ttctcgtcaa
    15181 tcgtactttt aattataagt aatctacagc atttcaatcg attattaaaa ataatcatga
    15241 taactaaaga tacaaactct atatattggc atattaattg cttttataat acctatataa
    15301 aaatgaaagg gagtgatact agatgcacat cttaaaatca gtgaaaaaga taccaggagg
    15361 acttatggtc tgtcccttgt tattgggagc actatttaat acagtatgtc cacaagctct
    15421 tgaaattgga ggattcacaa catccttatt taaaactggt gcaatgagta tacttgctct
    15481 attttgtcta tgcaatggag ctcaaatcaa tatcagacaa gctggtaaac cattgactaa
    15541 aggtatagta cttacagcaa caaaatttat aattggtgca gtattaggta ttattgttag
    15601 taaatttatt ggacctaaag gtataattgg tattacacct cttgccatag ttgctaccat
    15661 gactaattca aatggaggtc tatttgctgc tcttgcaggt gaatatggag actccacaga
    15721 tgttggagca atatctatct tatctctaaa tgatggtcct ttttttacaa tgcttgcttt
    15781 tggagtaact ggacttgcaa atgtacctat aatagcacta tttgcagcac ttataccaat
    15841 actacttgga tttatacttg gtaaccttga tgaagatata cgagaatttc ttgcacctgg
    15901 tacaacatta ttaattcctt tcttcgcttt tcctttagga gcagcattaa actttaatca
    15961 aattataact gctggactac ctggagttgt acttggttta ggagttacta taataacagg
    16021 tatgggtggg tactttgtaa tgaaacttat acgtgcagaa catccagcag taggtgcagc
    16081 tacaggtaca acagccggaa atgcagctgg tactcctgaa gctttagcag ctatagaccc
    16141 tactctagct acaatagcag ctgtttcaac agtacaggtt gctgctgcta ttattgttac
    16201 agcaatatgt tgtccaatgt tagtttccta tctagataaa agagaaaaaa agaaaaaatc
    16261 tatttttaaa acaaataaaa atattgaagt aaattaatac ttaaggaggt aaaattaatg
    16321 aatgcaatta ttcatgcaaa tgaaaaagat aatctagtta catgtgtcag acctttagtt
    16381 aaaggtgaaa aagtttcaat tgatggaaaa tcttacatag taaatgataa tataccagtc
    16441 tttcataaga tggctacaga agatgtaaaa aaaggtgatg ttgtatacaa atacggtgaa
    16501 attataggaa ttgcaacagt tgacataaaa actggtgact atgttcatgt acataacatt
    16561 gaaagtacac gtggtcgtgg agataaaaaa taggaggaag ttaaaatgaa attaatgggt
    16621 tatttaagag aaaatggtca gtttggtatt cgtaatcatg ttttaattat tccaacatct
    16681 gtatgttcaa gtgagacagc tacaagaata gcatcacttg tgcctggagc aatagcaatc
    16741 cctcatcaac atggatgttg tcaaattggt tctgatatag agcttacagc aaagacatta
    16801 ataggatttg gaaaaaatcc taatgttgcc gcagttttag tagttggtct tggatgtgat
    16861 ggtattcaag caaaagaatt agcatctgaa atagctacaa caggaaaaaa ggtagattac
    16921 gttgttatac aggaatgtgg cggaacatta aaaacagttt ctaagggagc tgaaatagta
    16981 agtaaaatgg ctcgtgaagt atcaaaagaa gtaagagttg aatttggtat gagtgaaatt
    17041 acattagcac ttgaatgtgg tggttctgac ccaacaagtg gaatagcttc aaacccttca
    17101 ataggcgttg catcaaacct tcttgtagat gaaggtggaa gtagcatact aagtgaaact
    17161 acagaagtaa ttggtgcaga acatttgttg gctacccgtt ttgaagatga agaaatgaaa
    17221 gataagtttc ttaaatttgt aagtgatgta gaaaaaagag caattgctat gggagaagat
    17281 ttacgtagtg gtcaacctac tccaggcaat aaaactggtg gactttcaac aattgaagaa
    17341 aagtcacttg ggtgtatgta taaggcaggc aataagccat tcaaaggtgc tcttgaatat
    17401 gctgatattg tacctcctga taaaaaagga ttatatttta tggatactcc aggtcaagat
    17461 atagattcta tcactggtat ggtagcaggt ggtgcacaaa ttgtaatctt ttcaactggt
    17521 agaggtactc caacaggaag ccctatttca ccagttatta aaatcactgg gaatagtgat
    17581 acatacaaca aaatgccaga caatatcgat ataaatgcag gtagaattat aacagatggt
    17641 gctaaaatag cagatatagg tcaagaaata tttaatgaaa taatagaagt ctgtaatggt
    17701 aaacatacca aagctgaaag tcttggtcat agagaatttg gcatttacag aatttcatct
    17761 acattttaga ttgtaacaaa atatctttta taaatataat acttatgtag atacataagc
    17821 taaaagttct ttctaatact ataaaaattt tctatactga aaaggaactg tatcaaaatt
    17881 attttgaaaa cagttctttt tttaacctct tttactcaaa atattataaa ataagattta
    17941 aacaataaac gtatcttgat taatctctaa gaagacataa ataaagctaa atgtaaagtg
    18001 ggataaataa aatttaatat ctttgtatga aaagatattt ggtataatat gtctgagaga
    18061 attcttattt gtaaacaaat aaaggatgga ggatgaaaca aaagaatgaa ctttgaaaac
    18121 acaaaaaata aaaaaataat agctacttta ctattgacac ttcttttaat cgtgtcatta
    18181 acatgtgtca cttttattat gaatctaaat aaagtaacac ataatgaaac agaacgatat
    18241 cttgtagaag tttcacaagg tatttccact acaattaatg tgaaatgtaa aagtatattt
    18301 aggacactat attctatggg agatacttat tttgagatta atgatagtga aaaagaagtc
    18361 aatagatact tatcaaaaaa attaaaatta tttgaatttg attggttagg tattatagat
    18421 tctaatggaa attttgtaag tgcccttaaa gatacaggta atgttaaagg acaagacttt
    18481 tatagaaaag cccttcaagg caaagaaaca attactcctg ttaaatcaca atctggtaca
    18541 tatggaattt tatattctgt accactatac aatggcgata aaattgttgg agctgttgct
    18601 gcttggaata ctattgatac tatgagaaat ttattaagtg tagaaagctt tcatggtgaa
    18661 ggattttccc aaattatcga ccaagatgga aattttattg taagttcagc aaacaaaaat
    18721 gctccaaaaa atgtggtaaa tttttttgac atgattaaac accaaggaaa acaggttgat
    18781 aaaaataaac ttgaagaaat gaaaataaac ttaaaaaaga acaaaactgg ttctatgtat
    18841 tatacattaa atgatggtat ttctaaggca atgcactatc ttccattaca agaagaaaac
    18901 ttatacctcc tttcaattgt tccaacaaaa acagcaagtg cacagtttga tttacttcta
    18961 aaaaaagcaa tcttcatcaa tattttaatt gttatactat ttttattgct tattatattg
    19021 attatgttta taaatcaaaa aaacagggca caacttataa aaatcgccta tgtagactct
    19081 gtaactaaag gttacagtaa agcacgtttt gatattgaag ctaaaaaaat tatacagtca
    19141 tcttcatcag aaagctattc attggtatct ataaatattc agaaattcaa acttattaat
    19201 gattcttttg gtagtgaagc aggagataaa acattaaagt acatacatga tgtcattagt
    19261 aattatctag aaccaggaga attgctttgt cgagtttcta atgaccagtt taatgtactt
    19321 attaaaacta ttcctcaaaa agaaattctt aagtacatag aaaatatcac caatgatatt
    19381 aatagtttca ataaaaatga agaatgtaaa tattttatag ctctatctat aggtgtatat
    19441 aaaattgatg acccaaatct acctttagtt tcaattcaag accgtgcaaa tgttgcacga
    19501 aaaaataata aaggaaacat tggaaatcat ccactttata catgtgtttt ttatagtgat
    19561 ttagagcgtt taaaaatgat taaagaaaaa gaaatggaaa atagaatgga ggaagcatta
    19621 aataataacg aatttatagt atatcttcag cctaaaataa gcctcaaaga taatactatt
    19681 gtaggtgcag aagcattagt aagatggcaa gacccaaata aaggccttat accaccagac
    19741 gagtttattc ctttctttga aaaaaatggt tttattacaa aactcgacca ctatgttttt
    19801 gaaaaagtat gtagtatgat aagaaaatgg atagataatg gagaaactcc aatacctgtt
    19861 tcagttaatt tttcacgtgc taatttaaat aatatagatt ttttagaaaa atacagaatg
    19921 cttcgtaata aatacaacat tcctgctaag ctcttagaaa ttgaattaac tgaaacactt
    19981 gtatttgaaa atctccaaaa attaattaat ataatagacc aaatccatga agaaggtttt
    20041 caatgttcac tagatgactt tggaagtgga tactcttcac tcaatatgtt aaaagaaata
    20101 aaagttgaca cattaaaact tgaccgtgct ttttttagct cacctaatgc agataatatt
    20161 gctgaaaatc atgtaataga atctatagtt aatttggcaa aaaaattaaa tatgaatagt
    20221 atatcagaag gtgttgaaac aatcttgcaa atggattatc ttaaaaaaat taattgtgat
    20281 atgatacaag gatatgtttt ttcaaaacct ctacctcaag aaatatttga gaaaatgact
    20341 tttggaaaaa aactagaaga aaccagtatg gatagttccg tttaaataaa ataaaaaact
    20401 tgtctctata aaataatttt aatcatttta taaagacaag tctttttatt ttccaattta
    20461 attttatttt ctaaatttct tacactcggc ttgttttaaa ttcccttttt aagaataata
    20521 atacgattat catagcaata aaatctgcaa ctggctgtgc caaccaaaca ccatctaaat
    20581 taaattgaat tggaagtaca atgattaatg gtataaataa aataaattgt cttaaaagag
    20641 ttaaaaacat tgaatgttta actttactta tacactgaaa atacacaggt cctacaatag
    20701 aaatccccat tataggcaat gtaattagat ttaggcttat acctcttact gctatatcca
    20761 tcaaatctga atctttatta aatataccta caaatacttc tgggaaaact tgtactacta
    20821 aaaatccaaa tgacaatatg acaactgaca ctatgataga taatattaaa gttttctttg
    20881 cacgctcata ttgttttgca ccataattgt atgatattat agtttgcata ccttgattca
    20941 atccaaacac aggcatcata aacatcaata aaatagatgt aagtgctgtc atagctccaa
    21001 tagctaaatc tccaccataa acctttagag aattatttgt aaacatgtga gttatactta
    21061 tagctacttc catagcaaaa ggtgtcattc ctattgcaaa taccttcttt actatatctt
    21121 tattcaattt taagttacat ttttttagtt ttaaattgga cttccctctt ataaaataat
    21181 gtattaccca aaaaaatact actatttgac agagtactgt tgctatagct gctcctttta
    21241 tacctaaatt aaatacaaat ataaatatag ggtccaatat caggtttaat acacatccta
    21301 caatcattgt ccttgctgct aattttggat taccttctgc cctaattgca ttattaagag
    21361 aaaatgcaac taaattaaat atacttccaa ccaatatgac actcatatag tcttttgcat
    21421 aaaatattga atctttactt gcacccaaaa taaaaagtat ctcttctaag aaaactaatc
    21481 ctaatatagt tataattaaa gagataatta tagataatgt caaagcatta cctaatatct
    21541 tctctgcttc ttctctattt ctttccccta actttataga caatcttgtg ctagcaccta
    21601 ctgataccaa cattccaaaa gctattatta gagtaaatac tggcatagtt acaccaagac
    21661 ctgcaatagc tatagagcca ataccttcca tagaaccaat aaaagctcta tctactacat
    21721 tataaagtga agttaccatc attgctatta ttgcaggtat cgaataccta aataataaaa
    21781 tccatatttt ttcatgtctt aatgcttctt gattctccat gttgtcacca tttacaccat
    21841 gtcataagtt atacaaattg gtttatttgt tcttgggtct gttgctattt ctgcatcaat
    21901 attaaataat tcttttagat tatccttcgt cataacatca tatgcatcac cttcacaaac
    21961 tattttacct ttcttaatac ctatcatgtg gtcagcaaat cttgctgcat tattaagttc
    22021 atgtattacc atgacaatag ttcttccttg ttttttattt agttcatcta ataattttag
    22081 tatctccaat tgatgtgcca aatctaaata tgttgtaggt tcatccagta ataatatatc
    22141 agtttcttgt gccaaagcca tagctatcca tgccctttgt ctttgtcctc ctgataaatc
    22201 ttccatattt ctatccttaa attctgcaat tccagtaact tctaaactcc aatttacaat
    22261 ctctctgtct tctttactag aattaccaaa tccactttta tgaggaaatc ttccataaga
    22321 aattaattct gaaacggtaa gaccactagg agcttgaggg ctttgtggaa gtacagccat
    22381 atcttttgct atttctttag acttcttttt gtagatgtcc tctccattta tatatatttt
    22441 cccactatta gggctaataa ttctacctat agttttaagt attgtcgatt ttccacaacc
    22501 attagcccct atgatggtag ttatttttcc ttttggtata tttaaattta aattttttat
    22561 aatatctgtg tttccatatg atacatttag attcttagta tttatacagt tcataacaac
    22621 accctttcct aatcatttac caacattaaa taaataaagt acggcactcc tattatagaa
    22681 actactatac ctactggtat ttctataggt gctagaagat ttcttgatat agtatctgct
    22741 attaaaagta ataatgttcc tatcaatgct gatactggta ttaatctttt atgctttggt
    22801 cctattattt ttctacctat atgtggagct attaaaccta aaaatgaaat actacctgct
    22861 actgctgtcg atacaccaga taaaattact gcatatatta taagtcttct tctttgtttt
    22921 tctacatcta caccaagact tatagatact tcatcaccca aatttaaaac atctatatat
    22981 ttagatttat aaatagttat acccataaaa atcactataa atggaagcac agctattaca
    23041 tatttccaac ttgttcccca aatgcttcct gatgtccatg ccataaccct attaaattct
    23101 tgtgttgtaa atttcaattg gaatatcgtc aaaattgaag taaaagctat gtttacacct
    23161 ataccaataa gtagtaatct tgacgaattt ataccacctt tccatgcaag agaatatata
    23221 agaaatgctc caaataaagc tcctataagt gcaacaatag gcattgtaaa tacagtcaag
    23281 ttactcatac cttcatatac attaccattc ataaggtata tataaactac tacaaacaat
    23341 gcagcacctg aatttattcc caaaattcca gaatcagcta aatcattctt tgtaactcct
    23401 tgaagtatta cacctgctgt tgataatgca gaaccaacca aaagtgctat tactattctt
    23461 ggtaatctta atttaaatat tgcaatttca tgattacgct gaccctgccc taaaaaagtc
    23521 tttataacat ccattggttc tatagaaaat gagcccatat ttaaactcaa taaaaaagtg
    23581 ataaaaataa gcaccattaa aattgatata acaagtaatg ttttagaatt tatttttata
    23641 atatttttca tagtacctat ttctccccct ttctaacaag gtatataaat attggcactc
    23701 ctataagtgc tgttatagaa cctactggtg tctcatatgg aggatttatc atcctactta
    23761 atatatctgt atatacaagc aatacagctc ctaatactaa agagctcggt attatatact
    23821 tataatctga acctactatt cctttagcta tttgaggaac tataagacct ataaaaataa
    23881 tattaccaga cactgacaca gatgctccag tcattaatat tacaagtact atacatataa
    23941 atctaacaaa cttagttttt tgtcctaatc ctattgccac ttcttctcca agacttaata
    24001 tagttattct tggagccata ataatagcta aaataaatcc aataccacca gctacaaata
    24061 gcaattttat accttcccac ttcactccta caagccctcc tgatatccag aaacttaatt
    24121 gttgagatag gttaaaatac atagatattg ctgatgccaa agaaattagc aatgtaccaa
    24181 gagcgactcc tgcaagagct aattttacat tattaactct tcctcttgat ttagaactta
    24241 taaaatatat aaataatcca cttatacttg ctccaataaa tgcaaacatc attacactaa
    24301 aacttccatg tataagttga ggaagttttt tttgaagtac cagtgatatc gaaattgcga
    24361 atgtagctcc ttgtgtaatt cctataacag atggttcagc tataggattt ctcgttattc
    24421 cttgcattat tgccccagaa actgctaaaa accctccaac aagtgcagct gctattgccc
    24481 ttggaagtct aacatccttt actattctca tatttattcc atcttccatt tttatcaaac
    24541 tatctataac agttgataga ttcatatctt ttgccccaat agttatggat aatacaattc
    24601 caaatacaag tacaactata cttactaata tcaacaaata agttatattt tttttactca
    24661 gcttagtcaa ttatcctacc tccttttccc ttttaatcgt cagtataata tcatctatta
    24721 agtattttaa ttttaagatt tattaaaaat aggtgtgaaa aatgaatatc attatctcac
    24781 acctactaag ttaagaatta ctttgttaat tcatttttaa ctgattctaa taataactct
    24841 tttccaattg gattgtatga ttgactgaaa aatggagcag catcaagtat tgttacatta
    24901 ccatctttta ctgctcttat ttgagcccag atagcactat tttctaaatc ttttttatct
    24961 gcttctgtag caattacaac tatatggtct gcatctatat ctgttaaacc ttccatactt
    25021 accattggta gtgttattcc atcctgtttt ggcatattct taggtctagc taatttcata
    25081 tcatcattga ttagagtacc tattccacca tcactaaata ccataaattg acctgaactt
    25141 gccaacactg gtaaataagt tttttctcca ttcttttcta taacttcctt acctaacttt
    25201 gtagcttttt catcatactt ctgtagccaa cttttatctt cttcttcttt gtcaaataac
    25261 tttgaaacat cagttaactt gcttctccag tcatttgcat aatctttcat cataactaca
    25321 ggagctattt cttttaattg gtcatatatt ttttcttgtc tttgactcat tattattaaa
    25381 tcaggattta cttctaatat agcttccata tccatagtat ccatcattga gtgcccaact
    25441 atctttacac ctttaagctc ttctcttatg taacttggta atttatcagt ttcatatgag
    25501 tctacatttg ctgttgcaac aggcttataa cctgctaata atagttcttc actacttcct
    25561 gatatatcaa ctattttttt aggatttgat ggtatcttaa cctctccttt aacagattgt
    25621 acaactcttg tctctgattt atctttatcc tctgtattct tatctgaaca tgctgtaaca
    25681 agtactaacg tagttataat agatataaat attgcaagtg attttatttt tttcatgatt
    25741 acttctccta cttgtttatt atacttacat gatattgatt atcataagca catatttatt
    25801 atagcatttt tctattttta gtcaatatta ttttgtaaaa ggataaaaat tcctaattaa
    25861 tcttaattat atatagctta gataaggttt tcttatatta aacagtattt tttttaagaa
    25921 ttggacacaa atacaatgac ttttcagtta aacaatgata aaatgcaatt tatagataag
    25981 gaatttagta gggaggattc aaaacatggt acatttagta tattgtgata atgcaggaaa
    26041 aaaaggagaa aaggtcttag ataaaatctt ggatggaaca aaaacaatgg ttattagagg
    26101 tgctgctggt agaaaaatac cacatagtag agtatttgaa gatgagttac tttattttat
    26161 ggaaaaagga actgcaaaaa tatctgcaaa agccactgtt aaaaaggtag aaaactatgt
    26221 taaattaaca gaagatgaaa tttctaaaat tttaaatgaa aatcaaccaa aattaaattt
    26281 atctgaaaag caaaaagttc gttggcataa aaaatgttta tgcttagttg aatttgaaaa
    26341 tgttgaagaa ataacacctc ttgattttga ccatcaagga aatatggatg attggcttat
    26401 tgttgaaaaa attgaagatg tagttgttgg tactagtata ccttacaatt atgagaagtc
    26461 taaattctaa agtatcaata acataatact atattgctaa actctactta aataaaaaaa
    26521 gagtgcattt aataattata aaaattattt ttatacactc ttttttaata ataattttaa
    26581 attacttact ttctaatctt tcatatacat ctataaattc ttctgctaat tgttgaaaat
    26641 tcatagctgt cattaaatgg tcttgtgcat gaataagtat aacacttatt tcagttttat
    26701 taccattagc ttcattttgt atcaattctg tttggaattt atgagcttta tgaattgatt
    26761 ctttcgactt ttcaatacat tctctagctt tatctatatt cccgctcttt gcttctttta
    26821 ttgcttcaaa tgctagccct ttcgcatcac ctgcaaatcc aattattcca aaagatatct
    26881 ctatcatctt ctcatccatt tattcgacct cctaaatatc tctatagctt gatatgacta
    26941 atccattctt ttctaaaaat tctttgactt ttttagatgt aagtatcttg tgctctttcg
    27001 ttctatcaat agcataagat gtagaattta aaatataatc atctaaaaaa gctgggtgag
    27061 acataatatc cactacatca tacttcatta tttcgtctat attttttata aaatattctt
    27121 cacttacatt ttccttataa aaactatcta ttaaaggaac tactttagaa tactctaaat
    27181 tatattcaaa tccacctctt attggaagat tatacttggt tacaatcttc tctattactg
    27241 gctttaaact tactaaagta tggatatgat ggtgactatc taaatgagat atatctattc
    27301 caagcccttt taccctatct atttgagcac aaagttctct ataaatctca tcacaatcca
    27361 ttttttcaat aatttcgtct gtaatcctcc taataaaaaa accgtcttta tcaacaatag
    27421 tttttaagtt agataataat ggtctaccac aacttaaagt catatgaact ccacaattta
    27481 aagttttatt ttctttaagt aagcctactc catgttctac cccaggcata tttgccatca
    27541 tagaagtaga cctaactata ccattattat gagctgaaat tattccatag ttcacagctt
    27601 cacaatatcc aaaatcatct gcatttatta taattttagt cataccataa cctactttct
    27661 atagacaact atctattaaa tctaggaaga taatctttat gagcttccaa catttcgtct
    27721 aatatagttt ttgctaagtc atcagatgga atcaatgggt ttatacaaag tgccaaaaga
    27781 gcagattcat aatcgccata tacagctgct ttagctgcca atacttcaaa tgatttgatt
    27841 tgacttacta atccatgcac agaatctggt aaatatccta tacttattgg ttttggacca
    27901 ttttttgtta ttacactact tacttctact gcttgttcat ctttaaaatc tcttatagaa
    27961 cccttattta atgtattaac tacttggata tcttttttgt cattgtatat agaacttatt
    28021 aaattacaag cagcatcact atagtaagcc cctcctcttt tttcaagttg aggtggtttt
    28081 acatctaatt tttcatcctt atataattca aataattgtt cttccaattc tttaactact
    28141 tgacctctag cttttccctc tttaaactct ctcaactcat cctcaagcat ttctttagtt
    28201 ttatagtagt atctatggta tggacaaggt attgcaccca aagattttac aaattcagca
    28261 ttaaaatcaa ttgcctttat attttgcata cttatatctg ctttgacaaa tttatcaatt
    28321 gcttcttttg taacatcttc tccatctaaa gatacattta gaccataaac catatgatta
    28381 agacctgcaa aatccatact tattctgtca ctttctacat taaatagttt ggcaacatca
    28441 ttttttaaat gaattggcac gttgcataga cctatatatc ttctaaaatt agtatatttg
    28501 aatacagcct ctgttattat tcctgttgga tttgtaaaat tcaccatcca tgcatttgga
    28561 cataattctt ccacatcttt tattatatca aatataactg gtattgtgcg aagggcttta
    28621 aataaacctc cagcaccatt agtttcctgc cccataactc catgagataa aggaatagtt
    28681 tcatctttta ttctcgcatc taaaagacct actctaagtt gtgttgtcac aaagtctgca
    28741 tctcttagag cttctcttct atctaaagta agatttattt tcatatctac tccagctttt
    28801 tttaccatcc tcttagctag atttccaact atctctaatt tttcttttcc agcttctata
    28861 tctacaagcc ataattcctt tacaggtaac tcttcatatc tatttataaa accctctaca
    28921 agctcaggag tataacttga acctcctcca attgtaacta tttttagtcc ctttttcatc
    28981 tcattatacc atccttacct ataatttaat atactctaca aaaccccata catttattta
    29041 aaactctata catttattta ccgaaataaa actacctagt tgttaatttt caatttatta
    29101 gcttccattt tttcagacgc tatgacaaat ggaatatata ttaatattga taatattaaa
    29161 ttaaatagtg ctaaagctcc gccagtccag tcacctgtag ccattgctcc acctaatata
    29221 ggaggtgtta cccaaggtat tttaggtaaa attactggtg ctactaatcc aaaatctatt
    29281 gctaagtatg atacagcact acatattact ggtgcaagta caaatggaat cataaatata
    29341 ggatttaaaa ctattggaag tccaaatatc aatggttctc ctatgttaaa tattgcaggt
    29401 ggaccaccta atactatcat ctctttacgt cttcttccag ctattaataa agctactatt
    29461 aatcccaata ccattccaga accacctaaa tatacaaatg catcaaagaa tgcacctgca
    29521 agtacatggt atccttccgt tgctcctgct tgagccaagg cagcattttc tccacctaat
    29581 ttcataagta tagtttctgt aaaaggaagt gctatatttg caccatgaag tccaacagtc
    29641 caaagtatat gaattataaa tgcaataagc attgtagaac ctagactatc agctacattt
    29701 gatagtggtg ctcctaaata agtatttaaa atatctgtta agaaacttcc attagaaagc
    29761 atttttataa atattcctat tacagtaaat atcattatag ttaacattcc tggtattaac
    29821 tttgcaaatg atcttgaaac tgctggtgga actccatctg gcatttttat agttaatttc
    29881 tttaccttag ataatcttac aaatatctca gttgataata atcctattaa tatagaagag
    29941 aataaagctg ctgtacctaa gtaggtttgt tgtatcattc cccatccttc aactactgta
    30001 gttcctccct caggaactat tttaccaatt tgtggagcca ttataaaaaa gatagataaa
    30061 gctataagcc cagattgtaa cccatcactc tcataagatt ttgctagaaa ataagcaaca
    30121 cctattacta agaatactgc cattgtacct attgctcccc accaaaggtc gcctccaaat
    30181 gtggtccagt tttctccaaa tatacttgcc atcaaatttt tgtatgcttc caatggtaag
    30241 ttatttatca atgttcccaa tgcacctacc atagttatag gtatcattac tataaatgca
    30301 tctcttactg ctactaagtg cctttgagct cctattttag cagctacagg cactatatat
    30361 ttatccataa atgacatgaa tttttccatt ttacatcctc cagtacaact ttattttgaa
    30421 ctatttaact ctacagctct atctaacaca gcttttccat tacatcttcc ataatccatc
    30481 atatttataa cttctactgg aatattatat ggttttgcca tttcagttgc ttttcctaat
    30541 acatatctaa cttgaggccc taaaagtaag acatcacaat tttctatttc attttttaag
    30601 ttcacttctg atatagccca tatttttgct tctataccgt tttcctttgc tgcatcctcc
    30661 atttttgtaa ctaataaact agttgacatt cctgctgaac aagctaacat tatttttatc
    30721 attttttccc ctccaaatta gattcattaa atatttttta agtcataagt tttttaaact
    30781 atacatttaa aatatgtttt tctattaatt ttttcttaat gtatatatct tatttttaaa
    30841 tgtatataca tttaattctt gatattatta tataccaaat gtatatacat ttcaagaaaa
    30901 ctttttcatt ttatttaaat tttattcgac aacagcattc aaagttaatg tatatccata
    30961 gtgatatgtg tgtgagtact caaaaatctc actattttgt aacttacaaa tttggcttat
    31021 atataaaagt ggctctggtt cttctaattt tagattatcc atacactctt cattcgatat
    31081 tacagcttgt acttctctct ctgacatggc tatcttgtat tcacacactt cttcaacaaa
    31141 attataaaga gaattttcac aatgagtctt atttaaattt ggaaataact taactggcat
    31201 ataagtaatc atatatgaaa ctcttgcatc attggctaat cttactcttt ttatttccca
    31261 aacattttct tctaaactta tattcaactt acttgataaa ttttcatctg cctgaactat
    31321 ttcaagagat accaatatat tagatacttt atagccttta ctttccatct ctttagtaaa
    31381 tccacttaca gacgatatat tatcatatat attatttgca ataactatac tacctacacc
    31441 tctttgtcta gttatatagc cttctttaac tagattatta agtgcagacc ttacagtcat
    31501 tctagttaca ttaaattctt cactcagctg tttttcagaa gggattaaat ccccttcttt
    31561 caaactgtct gaatttatta actctctaac atgattttct ataactttat aaattggctc
    31621 tttcatttaa ttcactcctt attaaatctt ttatataata agataatttt atatacaaac
    31681 aaagaaaatg tctatacatt taagataatt taaattttat tcagaaatct tgtacaactt
    31741 tccgagtttt aataaagata taaatagact tatcattaat atacctttta atatacttga
    31801 taaggttata ctccaccata caccatttag acccaacatt tctggtctag atataaagta
    31861 agccaatggt actcttgcac cagtcagtat agtaattata atagaaggta tataggttct
    31921 ccctaatcct ttgaaagctc ctgttgttat tatttctaaa cacataaaaa attgagaata
    31981 gcctaatatt ttaagataaa ctgctccttt ttctattgcc tcactttcat ctataaaaat
    32041 agaaaatata ctctctcctg caaacactaa tactaaagta gttattatac caagtacaga
    32101 agatacagtt atagctattt tgcaaccttt atttattcta gaatacttct tggcaccata
    32161 attttgtcca acaaaactgc ctaatgcaac tgatagccct tcagccgtca tccaggaaat
    32221 tgcttctatt tgtgagccta ctttttgtac tgcaacagcc acaggccccc atgatgctac
    32281 tattactcct aatagcattg agaatactgt aaacatacca ctttgtattg ctataggtaa
    32341 tcccaattta tacagaactt tataatattt agtctctatt tctttaaata gtcttaattt
    32401 aaaatattct tctttacttt taactattat atatataaaa catgtagtaa ctactatctg
    32461 agcaaataca gttgcaatag cagctcctgc aacaccaagt tttggagcaa atccccatcc
    32521 aaaaattaat atagggtcaa gtataatatt agttattaat cctactgtat taatacgaaa
    32581 aggagtttta ctatttccaa gaccattgaa aattgcagta aatactggat taataaaata
    32641 aaataccata cctagagcta ctattatcaa atactgtcta gacatggtta taacttctaa
    32701 gtctcctaat ctaaaaaaac ctatcaattg tttattaagt actattaaag aaattgtata
    32761 tattattgct agtataatat ttatttctat tgctgacttc atatatgatt tagtagtgct
    32821 tatattattt gcaccaatac tttgtgctac ttttatctct ccacctactt ttgaaatcat
    32881 aataaatgcc attgcaagcc atggaaaaaa accagctgtt ccaactgcag ctactgcttt
    32941 acttccggct ttaccaaccc atatcatatc tatcatgtta taccccatct gtataaatga
    33001 agtccccatt attggaagtg atagcttaag taatttttct gtaattcttc cttctgttaa
    33061 attaagtctg tctttcattt tccacctctt ttatattaaa tttatttgct tatatagcta
    33121 gattaatcat tttatttgat tgagttcact atccttcaat ttatattatc atatctcagg
    33181 attgcatctc aatcatactt atgtttaaga tacagtattt tacctatcta tgataaaatt
    33241 aatacttatg aatataaatc tagccatttc atcaatacat attctaaccc ttaacttaac
    33301 ttcaattaag tatacatatc gtaaccaatt agaatatttt atacggtatc ttccattaat
    33361 aaacaaccaa tatcaatgat ttatatataa ttttatttat acataaaata gtatactata
    33421 ataataaaaa aactatattt gaatgcttta agcatgttta ctaattgtaa tttccacaca
    33481 aatttgataa tatatgaata tattatggtg aaatttatag atatatatta attatgggga
    33541 gattgtggga ggttcttttc acaagacatt taagctatga aaataaataa aaaattcaat
    33601 agagttgata tgcaggtttc aatacttact gcaattattg taataatatc ttcctcatgt
    33661 gtatattttt ttaattatac actaacttat aagagcatga tatactcttt aagtgagagg
    33721 tcaaaacata tctatgaata tgtagaaaga aatattgata aaaatacctt tactgaaatt
    33781 aattctaaag aagaccaatc aaaaaagtct tataaaagtt ctaaaatcct tttagaatct
    33841 gtaaaaaata ctactggagt catgtatcta tatactgcta aaaaaacaaa agatggatca
    33901 ctagtatatg ttgttgatgg tttaaattca tctaggagtg attttagaaa tgctggtgat
    33961 ttgatagaaa aagagatttg gggagacctt aacaaagcat tagataatca tatattgtta
    34021 cctaaaaaga ttaaatcaac atcttgggga tacatattta tatcgtattt tcctattcac
    34081 aatgatgttg gtaaggttgt tggagtaata ggaattgaat ttgaagcaaa acatcaatac
    34141 aatacattta aatttataag tatagttaca ccaataatag cattattaac ttgtctaata
    34201 tcagcattga ttgcagtgat tctatttaaa agaatatcta atcctacata tactgatttt
    34261 gctaatacag attatttaac aggcctaaaa aatagaaatg cttttgaaat agatatgaat
    34321 aacttaaaca attcaaatct aaagaatcta atatctatca tatcaataga tttagacggt
    34381 ttaaaaaaga ttaatgataa atttggccat cagacaggag acttatatat taagcaagca
    34441 agtaaaatta tacaatatgt aataaataaa aaatatatag tttatcgtat tggaggtgat
    34501 gaatacataa ttattctaaa aaatttatct cacaaagaaa taaataatat tataaataat
    34561 attaatttaa aaatagttga agaaaactca actaatgaat taaaattatc aatgtctatt
    34621 ggatatgcta tttttgatga aaaagtagat gtttctttat ttgatacata tcgtcgtgca
    34681 gattctcaaa tgtatgataa caaaaggaaa attaataaac aatatcaata ataaatttaa
    34741 aaagtctatt ctttaaataa taaatcacaa gaatagactt ttttaaattt gtgtttttat
    34801 aagataaatt atttaattaa agtttgttct tttttctaaa cgattcttta taaattcata
    34861 tattactttc actaatatat ttgtgactaa tattaacaca gctagtgctg ctgcctgtgc
    34921 agtactcccc ttatcatcta gttgtacaat tgatattgca gctacttttg aacttgcagt
    34981 atatataaaa actaatgctg atattgtcac catggagttt aggaaaaagt acataaagtt
    35041 ctctaaaact gctggcaaac aaagtggtat agttacgttc ttaagaattg atgcgctgtt
    35101 tattcccatt gatttagcta ccatatctaa ttctttatct actttcttaa tagcagtagt
    35161 agctgttata aatgctactg aaaagaaatg aactatatta catataacca ttataagtaa
    35221 tgttccatat aatccattaa acaaattctt aatgtatatt gtatccataa aattaaactc
    35281 taatttatta aaaaataata cataagctat acctaaaact aatcctggca atgccatagg
    35341 caatgtactt aaaaaatacc caaatgattt taaatatgga gctttttctg ttttttcatt
    35401 caaatatgca aataagaaaa caaagctcgt ccctagaata gctgtaaata gtgacatttt
    35461 tattgaattt ataaatggtt taatcccatc aatactgttt ttcatagaaa aatgttccag
    35521 tgtcagtctt aaatcatatg gccaaactga tattaatgaa gataagatta cagttaacat
    35581 tacagctatt ataccaaatg caataattcc tacaacccct cctaaaatac tatctcttaa
    35641 cttattagtt ttaaccttat atggaattga ctttgaagtt atatatgaat tttgattttt
    35701 ttcaattttt ttagttacta taaacgatat tattgcaggt attaaaagta atattgatac
    35761 agttgctccc attggcatat tctgttgccc tataatttgc ttatatacat ctattgataa
    35821 tacattaaac tctcctccta tcacctttgg agctccaaaa tctgtaaaac ataatgtaaa
    35881 cgatacgaat gcagaactta ttaaagagta ttttatactt gggattgtta tgcttataaa
    35941 ttctcttatc ttgcttatcc ccatagtatt actagcttca tatagtctat aatcggttga
    36001 acttagagat atataaagta tttgaaatga ttgaggaaat acatatataa tttgtgccaa
    36061 tattattccc actgctccat atattggaac atgcactcca agtaaattag ttaccaaacc
    36121 ttgatttcca aataaatata ctagacctat tgcatgcatc attgttggag caaacatagg
    36181 aaacatagct aatgatttaa aaaaaccttt aaatttaatc tttgttctag ttaatgcata
    36241 tgcatatact aatgcaagtg ttagtgatat tacagttgat ataactgata caaatactgt
    36301 attttttaga gaatttaata gagattcaga agccaagtat tcttgaaagt tttgtaatcc
    36361 tacaaagact ccatctttat ctaaaaaagc ctgtacaaat agatatatta taggtgctaa
    36421 taaaaatatt actaaagaca taactaatac tgtaagcata cattttttta tatctaaatt
    36481 catttttgat ttaagactta tacttttaga acttatagca ttattattca tacacttatg
    36541 ctcctttaac ttttggatac tttaaatagt atttttcgtc tatacttatt tttacaaggt
    36601 caccttctac taaatttaaa ttaacccact cttttataga aacatcactt attatgaaat
    36661 tttcatttaa tttatctttt atttcaactc tcaataaatt ccctctaaac tctatatttg
    36721 atattatccc ttggtagttt tcttttgtcg atttctctat ttgaacatat tctggtctta
    36781 cagttaatat agaatctcca ttatcaaaac agtttgtatc tcctataaat tgagctgtaa
    36841 ataggttttg aggattttgg tatatttctt ctggagtccc tatttgcatt atatctcctc
    36901 catttagaat agctatttta tctgccatag ttatagcttc ttcttggtca tgtgttacca
    36961 ttattgtcgt tatattaagt tctttttgta atcttttaat gtccattcta agcttgtgtc
    37021 taacctttgc atctaatgca gacattggtt catctaaaag taaaaacttt ggttcaagag
    37081 ctaatgctct tgctattgca atcctctgtt gttgtccacc tgataatgct ttagggtatt
    37141 tatgagcttc attagtcaat ccaactgttt caagtacttc cttaacttta ttatctattt
    37201 tttcttttga aacttttctc tcttttagtg gaaaagctat attgttataa gcagtcatat
    37261 tcggaaataa tgcatatgat tgaaatacta taccaaaacc tctctttgat ggctctaaat
    37321 ttgtaatatc tttatcctgg agaattatag ttccactatt tacatcttca agacctgcta
    37381 ttattctaag taatgtagtt ttaccacatc cactaggtcc gagtaaacat agaaattccc
    37441 cttcttcaat atccaaacta atattattta aaaccttttt ttggtcataa cttttaaaca
    37501 cattatttat ttttaagtaa ctcatatctt atctctcctt atatagttat ttttttattt
    37561 taaattttat atcctacttt ttcttctaaa attctttcat ctaaattatt aatttaaact
    37621 ttacttactt tcagattttg aaccgtattt tgattcccaa gttttaagta ctgtacttct
    37681 gtttttggca gcagagttta aatcaacttc ttttataagc tgttcaaatg gttgtttaga
    37741 ataacctttt ggaactggat tatctgtctt tatggaagta actgctactt catgtgcata
    37801 tctatccata gctgaatcac ttattgccca atctaaaaat agctttgctt ctttttttat
    37861 attatctttt ttaactaagg catttgcttc taagtcccaa cctgaacctt cctttggaaa
    37921 tacaacctct ataggttctc cactagataa ttgttgtgta gctctatatc caagtgtaat
    37981 acctattgga tactctccag agccagctaa cttcgcaggt tttgaacccg agtgtgtata
    38041 tgtggctata tttttatgta atttatccat gtaattgtaa gcatcttttt cacccatcat
    38101 ttgaataagc cctgatactg taagataccc cgttccagat gatgctggat ttggcattgt
    38161 tataagtcct ttatattctg gttttattaa atcttcataa ctttttggaa tatctatacc
    38221 tagtttctta agttccttat aattaactac tattgctgtc tctgggacac tcattcctac
    38281 ccaagataca tcatcttcag tatctttaaa ttttttatca actttatcat aatttttagg
    38341 cttatatgct tcaagtgcac catctttttt tagctctatc atgttcattg ctgataatcc
    38401 ccatataata tctgcttgtg gattatcttt ttctgctaat agttttgaag ctattacacc
    38461 atgagaatcc ataactatct ctacatctat attgggatac tcttctctaa aaccttccat
    38521 ataataagct atctgctctt cctctagtgc tgcatatatt gtaagtttat tatttccttt
    38581 tccttgagta gtttgtggtg ttgttgatat gcaacttgtc ataaatgtaa tcactacaat
    38641 gcacagacaa gtaaatataa attctttcat atcttccccc tttgtgtttt ttcctttttt
    38701 tatttgtttt ttattgtttt tgcttatgtt tatataatac acctaatttg ttaatataaa
    38761 gttaagtgta aattaacagt tcgttaattt ttagaaattt ttgttaaata tattattttt
    38821 agttaaatac agtctgtttt taaacaatat attaattttt atagtatcta cattttaatt
    38881 taaacataaa aaataagtct aacctctata aagtagaatg ttagacttat ttttataagt
    38941 atttaattat acaaccaaat catttttttt cttttcttca agtgcttttt taagcaccat
    39001 atccttaacc ttcataagtc tagtagtact tgctctctca ttttcttcaa gcttcatagc
    39061 tatatactta atagtctcaa tgtaatttgg tatcattaca ttttcaagag cattaacacg
    39121 tctacgtgtc ttttctatct cttgtgcaag aagctgtgca cttttttcac tttctgcaag
    39181 tcttaaaagt ggttgcatag catctgagaa agcttccata gcagaatcta attcacctga
    39241 tgtaaaagca agaccataag gataaatatc actttgattg ttttcagttt taaagtcaaa
    39301 cacaggaaca tcaacactca ttatatttct cgtagaaaca tctactgaaa ctgattgctt
    39361 tggcatcata agtgctgaac ccagatactc ctgactcata actgctcttg cgattataaa
    39421 gtttttataa gcagtatcaa gagcattttc tgcttcttca cgcaatcgct tattttctct
    39481 tacaatctca agaaattgtt tcataagttc atcaagttta tctttaagaa gcttatgacc
    39541 tcttgtagca gttttaagga gtttcttaag tctagtcatt tccatacgtg ttggatttat
    39601 atttaatcta gccacagata tcactcctta ggcatatatt tttcaaaata ttcatcacgg
    39661 atacgtttaa gttcactttt tggtaacata ctaagaagct tccatccgat ttctaatgtc
    39721 tgctcaatag ttctatcagt tctaaatcct tgtgaaacat attctttctc aaattcatct
    39781 gcaaacttag catacattaa atcaatctca gaaagtgcag attcaccaag tatagccata
    39841 agttcttttg catccttacc tcttgaataa gctgcaaaaa gctggttcat tgtatctgca
    39901 tggtcttcac gagtcttacc tttaccaata cctttatctt ttagacgaga aagtgatggt
    39961 aaaacatcta taggaggctg tatatctttt ttataaagtt cacggctaag tataatttgc
    40021 ccttctgtaa tgtatcctgt aaggtcagga attggatgtg tcttatcatc ctcaggcatt
    40081 gtaagtattg gaatcatagt tatagaccct tcatgccctt tcatttttcc tgctctttca
    40141 tacatagtag caaggtcagt ataaagatat cctggatatc cacgacgtcc tggaacttct
    40201 tttttagctg ctgaaacttc acgcaatgcc tctgcatagt ttgtaatatc cgttattata
    40261 acaagtacat gcatatcttg ctcgaaagca aggtattctg ctgctgtaag tgccattcta
    40321 ggtgtagata cacgctctac agctgggtca tttgcaaggt ttacaaacac tacagaacgg
    40381 tcgattgcac cagttgcttt aaaatcgctt ataaaaaatt cagcatcttc aaaagttata
    40441 ccaactgcag caaatacaac ggcgaactta gaatcagttc cacgtacttt tgcctgtctt
    40501 gcaatctgaa ctgcaagatt ggcatgtggc aaaccagacc ctgagaatat aggaagtttt
    40561 tgccctctaa ccaatgtatt aagtccatct atagcagaga ctccagtttg tataaactcg
    40621 gccggataat cacgtgctgc tggatttatt ggagctccat ttatatcaag tcttttatct
    40681 ggtataattt ctggacctcc atcaataggt ctacccattc cactaaaaac acgacctaat
    40741 atatctggtg aaactccaaa gtcaagactc tttcctaaaa aacgaacttt actttcagca
    40801 aggtttatac ctgttgagct ttcgaaaagc tgaacaagtg cagtatctcc atttacctct
    40861 agaactttgc aacgacgaat ttctccattt gaaagttcaa tttctccaag ttcatcaaat
    40921 tttacacctt ctacgccgtt tattaacata agaggaccgg ctacctcttg tattgactta
    40981 tattccttta tcatagttct tcagcccccc tctttttaat aaggtctgca agctcgttgt
    41041 ctatttcatc ttcaattgaa tcgtaagctg catctatatc gccttcttct acatacttag
    41101 cacgaccaat cttttcaaga actgaaagct tgataatatc gtttatagta acattttgct
    41161 tgatagcatc ttgtgctgac ttatagaatt ttaatataag tcccatcatt ctatattgtt
    41221 tatgaagtga tgtataagta tcaacttcat ggaaagagtt ttgatgaagg aagtcttctc
    41281 ttatagaacg agtaacctct agtatcaatc tatctccatc tgaaagtgaa tcaaccccaa
    41341 ccaattgaac tatttcttga agttcagatt caacttgtaa aagtttcata gcttgagctc
    41401 tttgagctga gaaattatca tttacatttt tatctgccca tacatcaact ttttcctgat
    41461 agagtgaata actagtcaac cagttaattg ctgggaaatg acgtttatat gctaaagatg
    41521 catcaagacc ccagaatact tttactatac gaagcgttgc ttggctgaca ggttctgatg
    41581 tatcaccacc aggaggagaa actgcaccaa ttgctgtaag tgttccttct ctttcatcca
    41641 tacctagaca atttaccttt cctgctctct catagaattg agcaagacgt gaacctaagt
    41701 atgcaggata accttcttca ccaggcatct cctctaaacg accactcatc tctctaagag
    41761 cctcagccca tcttgatgta gagtctgcca taagtgcaac actatatccc atatctctaa
    41821 aatattcagc tattgtaata ccagtgtata tagaagcttc acgtgcagca accggcatat
    41881 ctgatgtgtt tgctataagc acagttcttt gcataagtga ctcgcctgtt cttgggtctt
    41941 ttaattcagg aaattcaaga agaacatctg tcatttcatt tccacgctcg ccacagccta
    42001 tatatacaac tatatctgca tctgcccatt tagcaagctg atgctgtgta acagtttttc
    42061 cacttccaaa aggtcccggt atagctgcta ccccaccttt tgtgattggg aaaaatgtat
    42121 ctataactct ttgacctgta acaagaagtg aatctggtgt tttcttttct ctatatggtc
    42181 tttcttttct aactggccat ttttgcatca tagttacagg tatatcatta cctttttcat
    42241 ctgtaataac acaaactgta tcttcaacag taaattcacc agaatatatt tcttttatag
    42301 tacccttaac tccataagga accataattt tacattctac tactgctgtt tcttgaacgc
    42361 caccaattat atctcctgct acaaccttat caccaacaga ttttttaggt ttaaattccc
    42421 acttagttgt tctatcaagt gaagatactt caacaccttt agttatatta gtacctgaaa
    42481 tatcatacat tttttcaagt ggacgttgaa taccatcata aatagttgaa ataagacctg
    42541 gtccaagttc aacactcata ggcattccaa gagaaactac ttcttcccct ggtcctagac
    42601 cggaagtttc ctcataaacc tgtatagaag ctttatctcc atgcatttct ataatctcgc
    42661 ctataagatg tttatctgaa actcttacga cgtcaaacat attcgcatct ctcatgccct
    42721 ccgctacaac aagcggtcct gacaccttta caattgtgcc tgttttcata ttctctcacc
    42781 tgcaatcata tactttatca aaatttaaat attccctcct atattttatt agcacatata
    42841 tttagcacca ataaaatttg agattgtctt ttattcaact ttcatctatt ctttaaataa
    42901 tatatctgca cctatagcac gtttactaga ttctcttact tcattcatac ctaatcccat
    42961 acttcctcca atacctggaa taactataat ggcaggaagt tggtaatctt tgtattttgc
    43021 tattgattct tttgcaagca atgatgcctg ttctgtaata taaattatcg catattcatc
    43081 ttcaaccaac ttatgtatac tttttttaat ttcgtcacta tcataggctg ggaagatatc
    43141 tattccaaga gctaaaaatc ccatgatgct atctttatct ccaactactc ctaccttata
    43201 cataagcatc ccttagcctt tcttttatag tatctgcgtc tatattatta agcttactag
    43261 tcaaaataat tctaattgtt tttatctctg attctttagc atataaatat gcaattagtg
    43321 gttctaatgt aagaggctta aattttgctc ctctaacata ttccataaga tagtcatcac
    43381 aaagtttttc aaagacagta aaaccactat ccatgccatt tttacagaca tcagagtaag
    43441 aagtagattt aaaacatgat gctggagtat ctgaggaaaa agcttctaag aatttttcct
    43501 tatcaagtga gccacctgaa acaaacacat tcataaacat atcaaaatct ttcttcatat
    43561 ttttgactct tataaaactc tttagatttg ttaaatcaca aactttcata acataatcta
    43621 ttacaaattt gttttggctt tctttagcag tttctttcat actggtaaag gttgctttat
    43681 ctagaacaat atcaaccatc tggccatttt gtgtcttatt ataaacatca aatgcctcta
    43741 atatagctga tgacattatc ttaggtaaat cactaaaact acgatttgct aaagattctc
    43801 taagcttaat aagaggaatt gtacctccat caattaaata tttctcacca tctacaccag
    43861 aaatttcttc tttaatcagc acttttaaat tgtgatagtc atttttataa agaaatacat
    43921 caacaaattt ttcttcagga gctagacttt taagaatttc atatgtcttt gataattctt
    43981 gagtcaaaat attctcaaaa ttatgtatat tattatttga tgtatcagca taacctgcct
    44041 cagctataat tcttaaagag tcttcagggc ttttggcttc tgccatttga ataaacattt
    44101 gtctgttaag aagctgtttt tcaagcactc ttattcgtgc aacggcatag ggataagttt
    44161 tttcttctgc cattttttaa cccctcctta attaaacaaa atttctgctg caatttgttc
    44221 aatatattca tgctctactg caattatggc ttcaaatgaa taattgtact caatgtcacc
    44281 ttttttaact atgaaacctc cagtaatatc tctagtttca tcagacactt taatgccttt
    44341 ttttgatagc acatctttta atgttttttt atctttttta gataatacta tctctgaatc
    44401 atctgtacaa tttgattttt caatcatgtt tagtataatc tcttcatatt cattatcttt
    44461 aagatttaaa agtttgtttt tagcttctaa aattacctct tctaaaatct cttgtttttt
    44521 tgataatatt tgctttttag cctccatata agctcctgat atttccttag aagccgcttt
    44581 ttctgcttct gcctctgcaa gctttgtata ggaagccatt tccttttcag ctttttcatt
    44641 agctgaattt acttttaaat ctgcttcact tttagcttta tccaatattt cttgtgcttc
    44701 ttgtttggca tcagctataa ttctgtcaat cattttttgt tcattaccca taggagtgct
    44761 tcctcctttt catttttttt acttatgaat tataatttat aattaaccaa ttgcaattcc
    44821 attgtaagct aagaaagaaa caagtagtcc taaaagagca tatgtttcaa ctattgctgc
    44881 aaatgttata gctttaccaa gttcttcagg tctttttgca actattccaa ctcctgctgc
    44941 tgctgcttta ccttgtgcta tacctgatac taaaccaact aaaccaattg gaagtcctgc
    45001 cataagaagt tgtaaacctt gagctgatgt tggtattgca tctccaccaa taatccctgc
    45061 ttttgataaa ataaggaaag ctatgataaa tccatacaga ccttgtgtac caggaagaag
    45121 ttgtaaaact agaagttgac cgaatttact agggtcttct gttacaacac ctgctgcggc
    45181 ttgacctgct attgaagtac ctttagcaga acccatacct gcaaataatg ctgctatagc
    45241 agctcctgat attgccaaaa attgaccatt tcctaacgcg tttaaaaatt ccatatttac
    45301 atctcctttt tattaatgct tgtatatttt gttttatact taaacggaac aaaaggaact
    45361 cctccgcctt cataaaactt attgaaaaat tctacatact gtaacctact tgtatgaaca
    45421 tatgcaccta gtgcatttat aagaaggttt attgtgtgtc ctacaacacc aattacaact
    45481 gcaagtatcg gaccagctat agcccctaaa aggttgataa cttgtgctat aactcctgtt
    45541 gttaaacaaa gcgccataat tcttgtataa gataatatat cagcaaaata acttgttata
    45601 ccatagagac ttgaaaatcc tttaaaaagc ttaacaggaa tacctttaaa gcttcttcct
    45661 tgagtaagta caagacctat agcacctata attgctaaat attttcctac ttctgaaaat
    45721 acacctatgt cccctgcaac aaaaggtatt ataagtagac atacacctgt tatacacaaa
    45781 taccaaaaac ctatgtcaaa gattgcatct attacctttc cacttcttat taatgtatat
    45841 gcttttattc ccaaacctac atatatatgg ataataccga acagtaacga cattcccata
    45901 agtgtcataa catcctctaa aggattaatt aatgctttta taggaattaa atcccccaaa
    45961 atacccccga atataagacc ccatacagta gttgaaactc cacagatacc aataagttta
    46021 attaaatttc cttcaccttt ttgtatcttt aatttatata caacaaatcc acaaactact
    46081 gcaattatta acccatatgc ggcgtcactt agcatcattc caaagaatat aacaaagaag
    46141 aaagttaaaa ttgtatttgg gtcaatatct tttgtacttg gatagctgta catgttagtt
    46201 acactttcaa aaggtgttac taatttattg ttttcaagca gaattgggaa gccttcttct
    46261 ttacttcctt cttcagtctg aacacaacaa tcaaactttt ctgtaatttc tttaattaat
    46321 tcatctgccc ttttgcttgg taaccaacca ttaagacaga atgttgtttt tgttttgaca
    46381 agtctctcta taatcttttt ttggtctctt tctatagtgt aaaaatcata taggttttca
    46441 agcatgtgta tactatctgc atgtctttta atttcctctg ttttgtcctt agaaacgttt
    46501 tcctgtctaa ttatcatttc attgtatttc ttgatagctt gtaacggtgt accttcttca
    46561 tttggtaatg aaaccactga aaaattaaat tcttttaaaa cttctaaaac atcatcaaaa
    46621 gtttctttta aagtaattaa ataaacatag ttcattaact tgtcagaatt gacaatccca
    46681 ataacacttt ctggtatttc atcttctaat tttactctta aagcatcaat gtttgcgctt
    46741 atgggcacag ttccaagtat agtttttgta tacttagttt ccatattttc aataggaata
    46801 tcaaagttca cccatggttc taacatactt ttattgttgt taagtaagtt tatgttagac
    46861 cttattgtac ctatctcctt gtgcaaattg tttaactcca gagcttcagc ataaacttgc
    46921 ccttcttttt cactatcaat tttgttaaac tctctttttg gtgcaagtag ggattttttt
    46981 tgtttcacta gtgaagaaac aatattaatt gcttggttta catgagacat tttctgctct
    47041 agcattataa cttcagattc ttcactatct tttacaagat aattttttaa ttcctcctcc
    47101 tcagtaagaa aggcactatc atcaatctga acaaacccac ggttcataag tagctttaaa
    47161 atctggcttt tttgagcttc taagccaaca atagatattt tgttcatatt aactattgcc
    47221 atattatctc actacctttc caataaccgc ttcaatagcc ttactttttc tttcttcagc
    47281 agttttttca attttcagac attcactttt agcattagct aagattttat caacctctgc
    47341 tctactgtcg gtgactgcct tctcaataag agtttcttcc ataagctttg cttgtttttt
    47401 tacatcagca aatatgcttt cacatttgtt tacagcatca gctctgatag actctgcatc
    47461 agtaacagct tttactttga tactgtctgc tgtctgctct gcctcaacaa tgctttttat
    47521 tatttcaaaa gccatccaaa tcacctctct taccttttta ctatgtatat aaccaattag
    47581 atttttttat tctaattaga ttactacctc attgttaaag tataaacagc atttttatat
    47641 aataattaat atttttcaac gtttatttct taataaaaca tattttagaa tagtacttgc
    47701 ctagatttac atcctttttt gacttacttt tagactatga catgagtatg gtttttaaat
    47761 accattattt tcaataatta taatttaaag cgatatagtt tgtcaatagc gttattttgt
    47821 tttaaaatta acgttatgtc gacattttaa atttttattt tatttttttt ataattttaa
    47881 tttaatttat tacatataaa agttttttat attctttttc aacctctaac cattgaagtt
    47941 tattgttttt tttgaatttt tagaaatatc tcgcactatt ttatctaact tttataaatc
    48001 atcaagctat atcgattttt gtcaccttat ttttaatcta atttttttat atctagttag
    48061 aaataaatca atatttttaa agattgataa aacttacacc tagttctaat agttttattt
    48121 taaggcaatg tattaatctt aagtagataa tatcattagt tactctatat attcgacaac
    48181 attataaaaa aatcctcgta ccaatcactc caatcttcaa taggatatgc tgctcctcca
    48241 tttacacttt tcagtagatt ggatgtatat tcttctccca tagaaaaaag ttcccctact
    48301 gtcatatcat aacctctaga tgtaacaacc ttacaaaatg aatccataac atcacgttgt
    48361 tctcttgttt ttataagttc ctttctaaat tcatcatcat tcttagcttc ttctaaaatt
    48421 ttagttaatt tttcattcat tatgtatttc tccttttaag tagtctttct gtaaacttca
    48481 ataagcttta tataaaaagg aagccatcct aaataaacta ggatgacttc gcttaactgt
    48541 tagcaacaca tttactagta taaaataatt ggtgtacctg gttctatatt ttcatatatt
    48601 ttctttgcct tagaaaaagg tgtatttaca catccatgtg aacctgaaga cttatatata
    48661 cttccaccaa aagaatttct ccaagtagca tcatgaatgc ctatgttccc attaaatgga
    48721 agccaataac ttacaggtga actgtaacct tgaccagtta atgaaacatt tctagcctta
    48781 taattaagag gatatatacc tgatggtgta gcaaatcctc tagctaaatc acctgttaca
    48841 acgtctgttg atacaagcat ttctccattt ttaaagaacc acatatgttg gccagaaaga
    48901 ctaatctcta catatgtatt tccaatatca tctttattac gagatacagc tttttgtatg
    48961 taaataggtt ctcttgtaac atttttacca gcttttacaa catcaactaa tgcttcagct
    49021 tctttctttt tatctatcaa ccaaccataa attccacctg aaattgtaat atctttacct
    49081 gtggcagttt taaatggtct tgagtcacca aaagtactgt attttttact taaacctctt
    49141 gtatattcta ccatagcatc attatcaaaa gttatgtcat aattcttttc tgagtctact
    49201 tttaaccatt tattaatttc agaaccatct aaagtttcct tacggtcact aaaatcatat
    49261 gtaacgacta caccaacata cttatctaat gtcttaatag cttgtttcaa cttttctgaa
    49321 tcctttgtgt tgtttggtgc ttcataaact ttactttcat ccagagaaat agaagtattc
    49381 ccagaattaa ttgagttttg tactaactta taaaaatcat cttttttcac tttgtttcca
    49441 agtacctctg gtactataac ataagcgttt ttagtgctat catacttagg ataagcactc
    49501 ttaggttcta caacattttt actatcaaaa caagataaag cattaaacct tttttctagt
    49561 aaatctttat tatatgaaag agttatcata ttattatact ctttagattt aaatattcca
    49621 ccaatccatc caatagcgct ctgactatct tttatctttt gaactttatc atctggtgta
    49681 aattttaaat caatatcttt tgcttttatt tgttctttct tattgtttct ttcctctaag
    49741 tttaaagtat aggaattagt actagatgct aacaaatcat ttacttgctc aactgttttt
    49801 cctgaagcct ttacgccatt aatagtcgtt ccaaagtaaa aatgatttct aaaaaagatt
    49861 gccattccta tataagctac aaacaaaaca cctactacta tagctatctt aaattttaga
    49921 tttttttgtc cactttcttg ttttacctct tttccatcaa tcataattaa atccttcctt
    49981 acattgtaat tacctatgta ccttattggt ttttaaacaa aaccatgtat gccttatttt
    50041 atatacccaa attactgtat cattatttat atttttaaat atatagttat ttctataaaa
    50101 ccattttatc ttttagataa aatgattatt ataaatcccc cctttcaaaa tcccttaaac
    50161 acaatctctg ctatattaat atattaccat acttttaata atcgtgggac tatctatcta
    50221 tttttgtaac actaaactac tattttaaaa aagaaactaa ttttgtgtta gtttctttat
    50281 cttaacttat ttttatattg aattttagct taacataacg ttgttatata gattctattt
    50341 aattatttta gtttctatat cttttccatc tgcatattct ttaagtaatt ccttagcata
    50401 tccaaaatat tcttcaatat cagattcttg actaaaagaa aatatacaaa ttaatatttc
    50461 atttgcatct tctgttatca ttgctctttc agaatcacta gttggactcc caaatttacc
    50521 cactgaatca gacaatatag gaagattttc tatatttatt aattctttcc caataccctt
    50581 atactgttct ccttcatctc ctacagtaaa acatactggt gaatgtaatt tgcttacatt
    50641 atatgtacct actggatata atgattttat tgatattaaa ttgtttattt ctactatatt
    50701 atttattgta taaagaccct tcccttgaat aattcttcta accaatgctt ctgaagatgt
    50761 tctatatcta cttggatttt ttccaagctc tttataagca tttcttgcat ctttaatttt
    50821 atctagtttc gtaatgtctt caacttttag actttgattt atagtatcac aaaactcatt
    50881 tatttcatta attaattttt ctgagctttt atttactgct acttttgctt gaatgcaacc
    50941 cagcttagca ttaggccatc tttccttaag tttgtcatca atacttatat ctatcatact
    51001 taaacaactc ctatacacta aatttttatt aaagttttta tcatctatac attatagtac
    51061 tattcttttg ttaataacat gtttattagc aaaataaatt tatttttaat ataattttta
    51121 taaaatacaa ttattttttg ctttatatta gacaatacat tgataaaata aaaaaacact
    51181 aattatttag caatgattta ttaaaatcaa gcattcataa tagtgcttta tataaaaatc
    51241 aaattttgtt ttaaagtaaa tctaaaaaat tcttagctgg cattggtcgt gcaaaataaa
    51301 atccttgaac taaatcacat ccaaattgtt tcattaactc agcttgttct ttactctcaa
    51361 caccttctat aattacaact aaatctagtg attttgctag agacactatt gattttgcta
    51421 tattgcgact tctctcatca tgttcaatgc caccaagaaa agacttatct agcttcaata
    51481 tatcaattgg catatctctt aaacaactaa tagaagaata tcctgtacca aagtcatcca
    51541 tagaaataga aaatccatac gtcctcaact catttaaaac attctttaaa atatttaact
    51601 cattaatagc cacagtttct gttatctcta tttcaatgaa atctgaactt aaattataca
    51661 tttttattgt tttattgata aattttacaa tatctggttg atacaactcg accctagata
    51721 aatttactga aactggaact atctttttac cttgcttttt ccattcatat aaatcattgc
    51781 aaaccctttc aaatacaaat ctcccaattt ttaaaatcaa cttacttttc tctgctattg
    51841 gaataaatac tgcgggactt ataaaaccat gttctggatg attccatctt attagtgctt
    51901 cactccctat catttctcca gttacaagtg aaaacttagg ttgataatac acttcaaatt
    51961 gattttttac caatgctatt tttatatcgt ctaaaatcat tgattcctca ctaagcttat
    52021 tcctaacatc ttcattataa atagcataat ttatattttt acctttagca acagacctag
    52081 ccatatttgc tttatttaca gccttttgaa tatctacttc accttcttca acaaaataaa
    52141 tacccacaca cagagaaatt tcaaagacta tactcaaatt actttcaata ttacttcgta
    52201 tattatcaag ttttcttata attttatttc ttccattttt ataatcacag aaaattgcaa
    52261 aataatcact tgaaagtcgt gcatatacct caccttctgt taattcttct tttaaagctt
    52321 ttgctattgt aattaataaa ttgtcaccaa attcatatcc aaaagtatca ttaatcatct
    52381 taaagttatt aatatcaaaa tatactataa tataatttaa agatggttta tctaaaagat
    52441 acttactaca ttctctataa aatttatttt gatttcctat atatgtaact ttatcttcat
    52501 aagccaaact taatatttct ttttcattag atttttttat ataaattata taaaaaataa
    52561 ttgcaacaaa tattaataca atagataaca ctgcatatac agctctgttg attatttgtt
    52621 ttgaattact aaatacaaca gtttctggaa ttgaaaaaat taaattccag tcttttatat
    52681 aattattttc actattccca ttatccatag tggcataccc taaatatctt ctttcttcca
    52741 acatatttaa tacagttgca cctgttttat tttcttgcaa ttcttttttt aatctattta
    52801 cttcttgaat atcattatcc tgttctaaca atttataaat attttttgat aggctatccc
    52861 cttgtggatg aaggataatt gtaccatcat tttttacaac ataagatata ctattatttt
    52921 cataaaaaga tatattaata agtttcatca gagtattgat attgtaagta caatataaaa
    52981 ctccaactac tttatcatta tttaataatg gtactgaaat agcaatcttc tttatagact
    53041 tatttacttc atcaacatat ggacttgaaa catacttctt acctttaatt gagtttttga
    53101 aaaattctct atctttcact gaaaacttat cacctttggt attttcagca ttaccattta
    53161 tatcaactat tgccatagtg taaaattttt gattttccat ttcatctaaa agatttttca
    53221 ttttttcctc attaggtata tctggatttg aagcataata gttagaaaaa atatttaaaa
    53281 catctaaatc tttttcaatt tgtctttgtg ctagttgaga gccttgtact gcaacttctt
    53341 ttatatgtat ttttgcctct gacataagaa gatgttttgt atcttctata tagatatacc
    53401 ccattgtcaa aaataatgat gttaaaaaaa gaattgtaat taatgtaaaa cttttatttc
    53461 tccctgtcat tcgactcctc ccctaattag tcaatacttc atatagataa tatatccttt
    53521 ttataccaat tcatcattta tattgtatat aactattctt aaatgttaca cataaaatca
    53581 acataataac cataaaaaca ttcttttatt attataataa cactatttaa ttgaatttta
    53641 aattaaagaa taaaaactat ccattttctc aaacaaaaaa actcttatac aataaaactt
    53701 tgttttatgt ataagagttt ttattttatc tattttatag caaacttatc ttccataata
    53761 tgaatttaaa tacatatctt ttatttcatt cataagtgga tatcttggat ttgctcctgt
    53821 acattggtca tcaaatgctt gaattaccat ttcatctatt gaatccataa acttagattc
    53881 ttcaacgcca aactctttta tagatttagg aataccaact ttaagtttta aatcttcaat
    53941 agctaccaat aaactttgga atttctcttc atcagaattt ccttttaatc ctaaaaatga
    54001 agctatttca gcatatctct ttaatgcttc tggatattta tattgagaga atgctcccat
    54061 tttctttggt gcatctgaag aattaaattt cataacttct gttatcaaaa gagcatttgc
    54121 aacaccatgt ggtacatggt ggaaagcacc taatttatga gccatagagt gacaaacacc
    54181 caaaaatgca tttgcaaaag ccatacctgc cattgtagaa gcatttgcca ttttttctct
    54241 agcttctggg tcttttgcac cattatcata tgcacgtgga agatattcaa atatactctt
    54301 aatagcttgt aaagctaatc catttgtata atcggttgca agcatagata catatgcttc
    54361 taatgcatga gttaaagcat ctataccaga agcagatgtt aagctttttg gcatattcat
    54421 catcatatca gaatcaacta tagccatatt tggcatcagt tcataatctg ctaatggata
    54481 ttttactcct gtatcttggt cagttataac agcaaatggt gttacttctg aaccagtacc
    54541 tgctgatgtt ggtatagcta caaaattagc tttttcaccc attttaggga atgtatatac
    54601 cctttttctt atatccataa atctcatagc taggtcttgg aaatctacat ctggatgctc
    54661 atacaatgtc cacattatct ttcctgcatc cattgcactt cctccaccta tagatattat
    54721 tacatctgga ttaaattctc tcatagcttt agcgcctatc ttagcacatt ccaaagttgg
    54781 gtctggttct acttcaaaga atgtagtgta cttaatattc atttcatcta ataaatcagt
    54841 aactgcttta gtatatccat tattgtaaag gaatgtatct gttacaataa aagctctctt
    54901 cttattcatt acatctttaa gttcttttaa tgctactcct aaacaacctt ttttgaaata
    54961 aactttttct ggtgctctaa accaaagcat attttctctc ctctcagcta ctgttttaat
    55021 attaagtaaa tgcttaactc ctacattttc agatactgaa tttccacccc aagacccaca
    55081 tcctaaagta agagaaggag ctagtttaaa attatataag tcccctatac caccttgaga
    55141 agaaggagta ttaattaaaa ttctacaagt tttcatggct gaagtaaact tatcaagttt
    55201 ttctctttga tttacatcat caatatataa tgaagatgta tgcccaaatc caccatcttc
    55261 tactaatttt tctgcttttc taactgcatc atcaaaatca cttgccttgt acattgcaag
    55321 tactggtgaa agtttctcat gagcaaaagc ttcttctatt tcaactgatt ctacttcacc
    55381 tattaaaacc ttagtatcta ctgggacttc aaagcctgca agttttgcta tagtacaagc
    55441 tgtttgccca actattttag aatttaatcc tccatttact aaaattatat ttcttatttt
    55501 ttctgtttca tctttatcta ataaataagc accacgctct ttaaattctt ttttgacttc
    55561 attgtatata ttttctaata ctataaccga ttgttcagaa gcacatatca taccattgtc
    55621 aaaagtctta gatacaagaa ttgagtttac agcagttttt atatctgcac tatcatcaat
    55681 tattgctgga gtattacctg caccaactcc aatagctggt tttccagatg aatatgctga
    55741 tttaaccatt ccaggacctc ctgtagcaag ggtcaaatcc acttctttca taacagttgt
    55801 agttaattct aaagatggct catctatcca tcctattata ccttcaggag ctccagctaa
    55861 aacagcagcc tcaagaacaa cttttgcagc ctctatagta gaattttttg ctcttggatg
    55921 tggagagaaa attattccat ttctagtttt taaagctaaa agtgctttaa agatagcagt
    55981 agaagttgga ttagttgtag gtacaactgc tgcaataaca cctattggtt cagcaatttt
    56041 agtcatacca aatgcttcgt ctttctctat aacaccacaa gttttagttt ctttatatgc
    56101 attatatata tactctgagg cataatgatt ctttataacc ttatcttcag caattcccat
    56161 accagtttct ttttgtgcca ttatagctag aggaactctt tgcttattag ctgccaaaga
    56221 tgctgctaaa aaaattttat ctacctgctc ttgactatat gtagcaaata ttttttgagc
    56281 ttctctgatt tttgctagtt ttttaactaa gctttcaacg ttatttacag tatctaaacc
    56341 aactgacata acttcatttt cttttttatt ttctattatt acttgttctt tatgagccat
    56401 tttaacaacc tcctgagata gttttttatt tattatgtta atttattaac attatagcat
    56461 ttacttttcc tattttcaag ttattttttt aacattttat tttttatcta atataacatc
    56521 atttctacta ataatatcta gctttacaaa tacttaattt aactagatat cactttattt
    56581 tttacgcaaa tcactaatga aatcatctaa aaacttaagg ataacgttat ttttttaacg
    56641 tttatatttt tgtatttttt gcaaataaaa ctttataaaa agaattttta atatgatact
    56701 ctatcaagtt taaaaataaa aatccttgaa aatgaaaatt aaaacttaat taacaagtta
    56761 aagtaaatat agttaaatgt acaaacattt acaacatata gtataattaa aaaaatatat
    56821 attcatgatt tttatataat atgaaataaa atatatttaa tattatatag ataaatttga
    56881 agtttataag aaggaggata agcatggaaa aattaaaaac aattagcgtt ttttctttga
    56941 ttataagtgt aattttaacg attggaggta ttggaatagt aacttattat gtagataatt
    57001 tgtttattag aggactatca gtatttgttt taattatgtc ctcttctttt gtatcaacaa
    57061 cagttagatt gatatttgaa gagtcaaaac gatataaatt ctaatttaaa aattggatta
    57121 agtaaataat tttaatatag caaaaattac ggagattagc tcaaaataga ctttttagtc
    57181 catgaggcat ttttttgtat gaaattaaat atattttcat catttcaaca agaaaaagag
    57241 gcttctctct attttgagac agcctctttg ctagtattgt atctaataca taatattata
    57301 agaaccaatt ctattgatgt ggacttttta taacaggctg tagctgtttg ttctctagag
    57361 caagttctat agttggaatt attagctcag tatgtgcaat catagaatta ggctttgctt
    57421 tgcaattatc ttgaccaaaa ggtacaaaat atatattttt agaattaagg agaatgccaa
    57481 tatttttgaa attaaagctt agagcatcat tcgttgatat tgatattact agtggtttat
    57541 cattacgaag atgacctttt gctgccatta atactggtga atctgtaata ccatttgcta
    57601 attttgcagc agtatttcca gtacaaggag ctattattat aatatcagca attcctttag
    57661 gtccaaatgg ctctgcctct tcaatcgtaa ctataggttt atttcctgta agttcatatg
    57721 cttttttcat aaactcttca gaatttccaa atctacaatc gatattttgt gatacatctg
    57781 aaaatatagt gtgtacatta gctccttctt taaccaaatt ctctaactct ataaatattt
    57841 tatcataagt acaaaacgaa ccagtaaatc ctactccaat tgtaagtcct tcaagtctca
    57901 tttctaatcc cttctttcat ttataatatt ttctatagcg ttaacaagaa tttcaccaga
    57961 tgtctttggt gaatatttac caggaagccc aaggcaaaga gtcgcattaa tccctgcttc
    58021 tttaacaaca gaataatcta ctcctccagg agaagaagct atatctataa tagttacttc
    58081 ttgtgatact ttttctaacc tttctttcgt taaaatacac tctggtatgg tgttaaaaat
    58141 ataatcaaat tgtaaaagtt tttcatcaag ttgacttaaa gaaatactgg aatatccaaa
    58201 tgcattagcc gtacttcttg catcattatt tcttgcgcca acacatacat ttgcatccaa
    58261 agcgtataat ttttttgcaa gtatttgtgc acatcttcca taacctaata ctaaacagtt
    58321 actttgatgt atattgatta tacttctttt aatagcttct gctatagccc cttcagctgt
    58381 agcaactgca tttaaaatag caacatcatt cattttcata aaatcataaa tctgaataga
    58441 gtgttgctcg caaaatttac aaactttagt tggtatgtta cctccaaata atatttgatt
    58501 aggagataaa ttaattagca agttatcaat agtagcatca agacaatcat ccgtattttc
    58561 aatattaact tgattctttg taaatggaat tggacataaa ataatggaac ttttggatag
    58621 tgcttcagct agtgaagttg attttccaac gattccattt ataatttcat ttgatatccc
    58681 ataagtgact acagaatacc ctttattaat tagttttttt accatgtaca cttgtcgcag
    58741 gtctccccca ataacagtta tatcgtattg atttgacata ttgtaagcct cctttactat
    58801 atacaatatg tttttatcaa tacattggtt cactaaaatt actaaatatt atacttataa
    58861 catttttaat aatgctaatc tatctatctt ttcagaagag gtatatggaa atgaatttaa
    58921 aaagataatt ttctttggga tttcattttt cattagatta tctttcattt taatcaacaa
    58981 atctttcttc gtaattttgt ctatagttat tacaaataat acaatttgat ttcctcttat
    59041 agtgtctgag tacggcaata caattgcatc ttcaatttga ggaatttttt taacttcatt
    59101 ttcaatctta gtagaactaa ctttaaatcc tccaatattt aaaatgtcat cacttcttcc
    59161 ttcaaatatt aagtatccat cattatcata atatccaata tcattgacag aataaggttt
    59221 ggttgcatta tatactgcat attctgtatt cacaaaaatt tttccttctc taatatatat
    59281 atctattcct ggaaatggtc tgcctacact tagaggtttt tcaattagtt catcatagca
    59341 taaatatgta atataattta attcacttgc cccatagtat aaaataattt cagactttgg
    59401 catatatttt tttagatttc ttgcagtatc ttcaaataat aattgagacc ctgtaaaaat
    59461 acttacaact ttgagtactg gttcttttaa gtgtttaacc aaaagttgaa gttttgtagg
    59521 tataaggtat atgttagtta cattatattg ttttattatc cttatccatg atttacaatt
    59581 aagacctgaa cttattacaa tactacctcc ttcatataat actgacatta tagaatttag
    59641 attccctgta aaactaagtg ttccattaac aaacaagatt gattccccag agatattaaa
    59701 aataccattt tgtataggaa aaaatccagc ccaactctca tatgttctat ataatacctt
    59761 tggaacactt gtagaacctg aagataaagc tcccatacaa atacttttat ctaaataatt
    59821 tataaggttc ttatattgat acatatatat attaaattta ggattaaaaa tagaaaaatt
    59881 aacttttttt gctacttcat tattgttatc atcatataaa tcaataacgt ccatagattc
    59941 atcagatata atgattgaaa tattattttt taataaaata tcatctaata cttttttaga
    60001 aagattataa tgacaaataa taggaatatt ttttgaataa ttaatagcaa aaaaactaat
    60061 caactgaaac attatatttt tagaatatat taaaattggg atattttcac ctaaagaaat
    60121 ttgttttcct agttcctcac tataactcaa tatattttca taagtatatt tttctccatc
    60181 tataattaaa aaatttttat ttttatgcct ctcttttttt attcttaaaa gttcataata
    60241 gttcataatc tacacccttt caagcagtaa agctgaacct agaccaccag ctgcagctat
    60301 agagcaaatt ccatatctac ccttagtctt ttctaatgct tttaaaagat gtaatgcaat
    60361 aattgcacct gaagcaccat atggatgtcc atacgctaaa gctccaccaa atatattata
    60421 tctatctatt aactctggat attttctttg aaacaacaca tctataactg caaatgcttc
    60481 attaaattca acagcagata catctaaata attaagactt tctatctcta aaagtttatc
    60541 cattgcttgt atcgctgatg ttggacttaa ggttgcatct gttcctattg tgcatgtatt
    60601 aataattttt gctttaggct tcttttttac atgttctaaa tacttcttag aacatattat
    60661 aataaatgaa gctccatcat taataagaca tgaatttgct gcatttgtaa tagtttcttt
    60721 tcctaaaatt gatggcatcc tatctaataa cctttgactc atcttatcac gtataccttc
    60781 atcataggta ctattattta taggagatat aatatcttca agtatttttt cctctcttgc
    60841 ttcttttgct cttttatgac tttcctttac ccaaaaatcc aagtctgctt tctcaatttg
    60901 atataactct gctactcttt ccgcaccttc gagcatacta ttttggctat tatcatcagg
    60961 agaaaattga gcaactgtat aatttggatt atttgtatta tatcgcttat cattcttatg
    61021 atatgtgcgc attggttgta gagaactact ttcaaaacca cctgcaataa ttaaatcaca
    61081 ctgaccagat ttaacctttg aaaaagctat gtctatactc atcatagcag aagcacactg
    61141 catatccaca gtaaaagcag gtacttcatt tgacactcct gctgttaaag tcattaatct
    61201 agctatattc cctcctgtcc caactgcatt tccacatata atctcatcaa ttttatcaat
    61261 ttcatacttt ttaattaaat cttttaaaac acttgctcct agtaattcag gttgaacaaa
    61321 ttgaaaaatt ccatttttta gacctatatg acttctcaat cctcctaata taaaaacttc
    61381 cttcatacat tacacccttc cttatattat agatttatca tagatttctt ctgagtacat
    61441 tgttcagttt agtgcctaaa aaagatgcaa taactgactt tattgtatct ccaattaaaa
    61501 atggtactac tgctgcaaaa agtgctgctt ctactgtcat ttgattaaaa tagcacataa
    61561 atactgttcc cataaaatat ataataggca ttccaacaaa aattgttatt agtatatatc
    61621 tcttaatatc attatttcgt ccttttaata aactaataat tagtgctgca aacaaaaacc
    61681 caaaataaaa accacctgtt gggcttaata actttccaat accagatgta cctccaccaa
    61741 atactggaat cccaattaaa cctatcatta tatatacacc aactgtacta aatgcttgtc
    61801 ttggtgttaa aatcaaagct attaaattta tcattattgt ttgtgctgtt actcctgctg
    61861 gagtaaatgg caatggtata tatatgtaag atgatataca caataatgct acacatattg
    61921 acattttagt taaatcttga atatttactt tagtattttt catttttata attcccttca
    61981 tatgtcttat ttcatttagt ttatatgtac atatacaaag cttctattaa agctttgttt
    62041 tagtataaaa taaagacgtt tttttgtcaa ctattataag catttaggtt tacatttaat
    62101 ttataagtaa actttatact gttactttat attttaatag tcatttataa aactttgctt
    62161 aaaaacagct ctatttagac tatacttgta aattataata tgatatattt ttatataaga
    62221 ttttatttta tataaaaaat agtaacaaat agtttataca caagatattt aaaaggaagt
    62281 gatgatatga aagttaatga aaataatata aataatatag atgaagagtt agatgaactt
    62341 agtaggaaag tttacttaga gtcaccagat ttttggataa gtatttctga caaaataata
    62401 gataaagtat ttgatgaaat gttattattt tttgcagtta aatataacta tttcaatata
    62461 gtatgctttg ctattgaaaa caattatctg gatttaaaca tgccttcaaa aaacaaaaaa
    62521 tacactaata taatggagca tttgttagat acatcaaaac agaatgaaga taaaaaaatt
    62581 tataattatc ttctaagctt aaataaaggt aataacctag atgaaagacc aattttagat
    62641 gaattaaact gtaatattag tagtgatact tttgaaaaaa aagatatata tcttccaaag
    62701 tacttatgtc caagttgtaa ttctaatata tttgatacag gttataaagt atcaaatgac
    62761 ataacatata aattttcttc taaagaagct gaacctattg aaatgtgtag tgaagtttct
    62821 tctatcagat gttgtaattg tgatgaatta ttagaagata ttacacttga taaactatat
    62881 aacctatgca aaattcaaaa ctgtaataat tgtggttcta atttaactaa ggtaggaatt
    62941 attgcaaaga aaaaaatgaa ctttgatgag aatactgaaa aatttaaaga tgtctcaaca
    63001 tcttactgtt gtggaaattg tgactctgaa ataaacttat ctcaaagaga acattttaag
    63061 atttaaatat ttataggcta aagtaataag attacattat tagatactgt ataagttttt
    63121 taaatctgta aaaataaata cacttataag aattaaaatt gctatgaaac tagttcttga
    63181 tgaactaatt tcatggcaat tttctataat ctaataatta ttaaatttta acaaaccttt
    63241 attagcattc cataattatt taaaattctt tcaaataccc tatagtttat aggtgaaagt
    63301 atctttaata ttatatcaaa cttattatct ttaaatagta aatttgttaa atctgaaata
    63361 caccatacaa ttttattttc ctcttttgta gctatatcaa ttctgattaa cttacatcta
    63421 taataaatat ctttatcttc atatagtcta ttttaaaaat tactcttaaa aaatcaaatg
    63481 gcaaataaat taattatcct tttaatagaa tgtaatcata ttatttacta tattccatta
    63541 tgtactcttt aaatcctgaa tcttcattta cttgttcttt tactatatta aaaccttttt
    63601 tattataaaa cacaactgct tttttattat ctttatatac tgctaagctt agacttttat
    63661 attttttagt tgcataatct aaaagtttac ctccgatacc tagattttgg tatttgggct
    63721 ttataaataa agctcctata aagcttttat ctattatgct tataaatcct tttatttcat
    63781 ctccatcatc atatacaaat gtatctgata taggtatata ttcgttttta acagtattat
    63841 agttattttg ccagtattct ttacttataa agtcatgtgc tttgattgta cttttttccc
    63901 atatttccat gattttattt atatctttat tgtttaattt ctttatcata aattttattt
    63961 cacctcattt ttgtattgat tttataatgt ttataggtaa gtttatttta tttagatatg
    64021 atttagttaa tataaggtgg atgttttagt atatgctatt gttgattcta caattttata
    64081 tactgcttta ttaatataaa ataagtttct aaatactcgt tgatattgct aatattctac
    64141 ttatctacca cttagttaat ataaaacaat gatactgtaa tatctaaaga tgttacattg
    64201 gcaaatttac ataccactta gttaatataa aaccatcttc agtatcatat gctgaaatat
    64261 tatcaagatt tacataccac ttagttaata taaaacggca atcatgtcaa gtacttctaa
    64321 tataaaaaag ttctatttac ataccactta gttaatataa aacttcaatg aactagggtt
    64381 taaaatacat tctagcccga atttacatac cacttagtta atataaaact ttaaaattcc
    64441 ggacaaacaa cgctaaatca aaagaattta cataccactt agttaatata aaactaatag
    64501 aattatagca tcaactaatc gtaaaatatt tatttacata ccacttagtt aatataaaac
    64561 ttgcggtaag actgtttgtt acagggatgg cagagtcatt tacataccac ttagttaata
    64621 taaaactcaa ctctccttga tagatttagc ctatcttgcg atatttacat accacttagt
    64681 taatataaaa cgaacaaatt taatggaagc aataaaaagt aatgacgatt tacataccac
    64741 ttagttaata taaaacggag gaactaaatt tagcttactt tgataaagaa agatttacat
    64801 accacttagt taatataaaa ctcaatatct tttcttatta gtttcgcaaa ttcttcatat
    64861 ttacatacca cttagttaat ataaaacgaa ggcaaaaact aatgactaat aaagagaaaa
    64921 taaaatttac ataccactta gttaatataa aacatattta aagcaagtac ttttttaaga
    64981 aaacttaatt tacataccac ttagttaata taaaacatgg ctaagagata cagataaaaa
    65041 tataaatgaa tggtatttac ataccactta gttaatataa aactattacg agaaaaattt
    65101 aaaaacgact aaaatccata tttacatacc acttagttaa tataaaactc catttatttt
    65161 atatattaaa attatagcat tcgaatttac ataccactta gttaatataa aaccttttgt
    65221 tttaggatga ttatataata ttacaaattt atttacatac cacttagtta atataaaaca
    65281 agcagaaata ataggactaa tatctatatc aaccaaattt acataccact tagttaatat
    65341 aaaacgatag aagaaaataa tataaaagtt accctaaatt aatttacata ccacttagtt
    65401 aatataaaac ttagaagata tagaagttct tgtagatact gttgagattt acataccact
    65461 tagttaatat aaaactaaat ttaaaataaa ttattaaata tccgaaaaaa ttatttacat
    65521 accacttagt taatataaaa ccccaaaata ggtatatttg aatacattga aaacacttca
    65581 atcaagcaat tataattcaa taaaaacatc aaaatttgca gtgagcgagc agtagtgcaa
    65641 ttgataacaa ttatcataca ccttcaatcc cttatattcc aactgttaac acctatttta
    65701 gcataaatat cgctcactgc aaacctatat aaacatttca tcagaatact tatcaatccc
    65761 ataacatttc tttttaattg aattaggatt ttttatctca taaacataga ttgaatcctc
    65821 gctattatca ataatatttt ctatttcaga aatacattta tgtaactttc cttcagtaat
    65881 attgccttca aaaacagaat tttgcgtcca agtcaaatat tttctaagta tccttctaat
    65941 tctatttaac gaccttgctt caacaatatc ataagtaaca ataacaaaca tacttaccac
    66001 cacaccttta aaactttgta cctttcatca tttataaaat gtttaattaa tttataacac
    66061 tctaacctta taaacattct atatgaaact tttctattta gatttctatg ttttatagtt
    66121 gtctgcatct tattttcaaa ttcttgcaaa aatttcttct ttcctttttc acttaaataa
    66181 caaattttgt cttgatattc aaaatcattc ttattaattc ttttattatt aatcaggcta
    66241 aaaattattg tgtcaattat aattggctta aaaatttctg caatatctaa acttaaagaa
    66301 aatctctttg ttgatggttc atgtaaaaag cttattgtag ggtcaagttg tgtcttatat
    66361 atctcactta aaacattcgt atacattatg ctatttccaa aagacataag agcatttact
    66421 gggtctttgg gaggtctttt ttctctttta taaaattcaa aatcatcttt taatatttga
    66481 ttaaatgatt tatagtatgt ttttcttatt ctgccttctt tacccataag ttctgatatt
    66541 tctttgacat cattcaaatc atttagttca tcctttatag tatcaattaa actttcatca
    66601 actttgtaat atctcatatt tcttaaaata tggtatgatg cactttccac aaaggatttt
    66661 gctaagtata atctttcatt tttatctaaa tagcaggctg attgacatac taaactgaat
    66721 cctgatacat ttttcttcct tggataatat gtacctgaat aatatccata gtaattgtag
    66781 aaatgaagca tcaccccata tttgcttatg taatttataa gttttgtatt taaatctact
    66841 tctccatata tatgaatatc atcaacttgt tctactggta atattttttt ctcaccttct
    66901 aaattgtaat agtaaatcgt attttctttc ctttttatat ccccattact tataatataa
    66961 tagtctttat acattgttac tcctccttca caaaacaaaa ttcataataa gcacattttg
    67021 tacaataatg ttttttctca attttgggag gagataactg catatttatt tcatttatat
    67081 ttataagtgt gttttcaata attctttctt tttcttctgt caatatcaat tcttcttgtt
    67141 tcttttcctt aacataattt atagtacctt ttttctctat acccatacta ttctttaaat
    67201 agtataagta atagtataac tgcatttcat ctgcttcttt catctttgaa cttattttta
    67261 cttcttttac atactcatcc tttattatat ctagcttcaa gatatcatca atcaatattt
    67321 ctttattttt cattctttta tatgaatctt catgtactat cttcccagac aatactctac
    67381 tactattttg ttccatggtt attcctttag aaaatagcca tagttttctt ttacatacat
    67441 aataataatt tatttttaca ccttgaactt ttaatacttc taaatctatt ggcacaaaat
    67501 caccttcttt agtaaatttt agccacattg ttaatataaa aatcaaataa attggtctat
    67561 ttcattactt ataagcacac ctttaccaaa tatctgtttt gtatgcttat tttcgtatat
    67621 atcatattta tataaaagta tttttaaatc ttcaagacct ttcacctgta tgtcaattac
    67681 attttgttta gctttatata ttggtaaatt tacagttttt ttaattatct ctcttcttgc
    67741 tttttttctc ttagacttta ttttatttat caattgtcta tcatcattac tatatgcatt
    67801 tataagctct tgtcccaact cttcataatc tttgattaaa gtttcttcaa ttttattata
    67861 tatatcctct ggaattactg tatatccttc aatatttcta agtatatttt gtgcatcttt
    67921 agaacctaaa ctatatggcg ttatagtatc taaaatattt aaagcactgg taaatctctt
    67981 ttcaaaagct gtaccttcta gactttcttt agagtatagt atcttaacca tttctacctt
    68041 atatttttct ttcatcttca cacattcttt tccatttata aaagttttca gtagctcaag
    68101 tcctttttct acaatatcct tatcataaat actacctatt cctgtagctt tttctgtata
    68161 tataaatata tttgggctat tttcttcata ctcacgactt ctataacatc taccaaatcg
    68221 ttgaaataga ctgtcaagtg ttgaattttc tgtatgaagc tcatcaaaat caatatcaag
    68281 agatgcctct actaattgtg ttgtaatcca aattccattt ctatcactat tggcaaattc
    68341 ttttatatat ttttctaatt ttgctctatc ttcttgtata tacatagaat gtaaaagatt
    68401 taagttgaca tctattcctt ttggtttaac tatttcttct attaactcat atttttctac
    68461 agcactttta actgtattta ctattacaag aactttttta ttcattccac tttgtattat
    68521 tttacctaag ttttcatcta ttgaattttc tacaatagaa acacaatgtc ttattttttc
    68581 tgtattacat gtcaattcag ctaagttact attcattaca cctctttttt ttaattcatc
    68641 tatatatatg gttggcatag tagctgtcat tatcataaat ctgccaccta tcttatgtat
    68701 catttctata ccttttacca atacagctgc tatttctggt gaatatgctt gtatctcatc
    68761 tattactact tttgaatatg ctaatgttga gtacactttt tcataccctc tatacaaaaa
    68821 aggaaattta aatatttggt ctattgtaga aaatgtcagt ttgcaagata ataactttgc
    68881 taaatctaca atctcacttg aattttcttg attactttct tctagataat ctattgctgt
    68941 tgaatgtaac aaacctaaga atgtatcatt tgattctcca acaccaacta tattttttgc
    69001 tctatcaaat aatgcattta tacttactct taatggaagt gtaaaaaatg ccttgtcttt
    69061 atctatccaa attaaggcag tttctgtttt tcccattcct gtagatgcaa tcagtattat
    69121 attcttattt ctattagatt tagcaaatga ttgagcctct ctcaaactac caaactcttt
    69181 cattaaataa ttttctgtct gttctcctat atttataaca ttattgcatt ccacaacttc
    69241 atgagcagaa gcactatggt ctagtctatg tagtattcct tttaacataa tatataaatt
    69301 atagtacttg tgatttttat ctattctttt ttctacactt tgtagatata ctttactcaa
    69361 tttttctgtt tttattggat atctaacttt aaattcatgc tgtagttcat aaactttatt
    69421 tattaaatct tcatctaata ttttttgtat taaattttta aaatctttat ctataaagat
    69481 atctctttca tgatgatata caataacttg atttaatact gctctaagtt ctttattttt
    69541 tttcctgtct atataactat aatcaataaa tgcaggagaa agataattat ggcctacatt
    69601 attttctaaa taagttacta ttttaggttc atttatttta gtatccattc tgctttttat
    69661 taactcttga aacggtgaaa atgcttttcc aatatcatga aattctataa caaagtcaag
    69721 taattgccaa aatatctcct cttctagaaa atctaagcta tttatatttt ttccataact
    69781 ttctcttaat acattcattt gttttaaaag ttcatcagta tgttctctaa gtgtttccac
    69841 tggattagat ttagcatata acataatctc cctcctaatt tatattatta aaatataata
    69901 aatctccttc attatctaat aatatatctc caattgatat agtatttcca tcctcaacat
    69961 aataactatc taccttattc caaacccttt ttttatcttc cacagcatta ttatcatagt
    70021 aattattgag tctgtaactt ataccatcta tatctgtatc ttgtaaactt gtttttggta
    70081 tatatatagg cattcttata ttacattctg ataacttata attttcataa tcatctaatt
    70141 gtatatcctc atctacttca aatttattga cttctacaaa tctaatatca ttaattctta
    70201 ctaaatcttc ttttctacct aatgataagt gctcgtcata atttaataaa ctatcatata
    70261 tttcttccaa taaatcaaaa tccccaccta tatgaattat caaatttaca ttcaatagca
    70321 tatgactatt cattggcata gatgtaatac tatctttttt ataaaaatat gttgtctggt
    70381 aattattaaa tatactttca tatgttccct gtacacttat attaaatggt atatattctg
    70441 tagcatttaa tattttgtga attaatccat ttattgtaga atatggaggc agtggatatg
    70501 tctctgtaat cttcatagca aatggttttt tgtagcatgc agtttcttga aataaatcta
    70561 atttcaaaac cttcatatta attcacccca taatattcct ttacttcttt aactagttgt
    70621 ccaaaaaatt tatctacact taaaaaatta tcttcaaaca atcccttaaa ttcttcttta
    70681 tttataaata ccccatctac cattccaaca taggtaaatt cttttaaatc ttttcctaaa
    70741 aaagtacctt gaattacttc ttctatagct tgcttattta ttttaaatcc atttttatct
    70801 ccttttaatt taattctacc caagaaaaat ggatttgcta tttcatatac tcctcctact
    70861 acaaatagag gagataaatt ttcttgtcta cctctaatat ttctattcaa tacttttatt
    70921 atttctaaaa attctactac tcttctacat ttctctttat tatcaagttc tatatctcca
    70981 tccttaccta ttttagataa atctatagtc actgtatatg tataataact taaatgttgc
    71041 tctatgttag caagattagg atgttctcct attctatctg ctaatccttt attatttaaa
    71101 aagtccatgt cacttctata tggctcaagg gaaattgcat tacttaatct tacagtagct
    71161 tctctagtca aagaacctcc ttttttatct ttctcatctt ttttatttgt tttcatatat
    71221 ccaaataaat ccatttcttc tgattctctt atacttatat tgtctttaaa ttgaatagtg
    71281 cctttttctt tatcgacaac ctgtaaattc caattaaaca attctgctgc taatcgtact
    71341 atgtcatatc ttaaacactg gcgagatgca aatgtatata tatttccatc atctctactt
    71401 aattttttta attctgatat attacctatc ccttccccat agtttaaact ttgagcctgt
    71461 gctattactg taaatgttaa ccctcttttc atcttatttt tcctcctttt cgtttggtag
    71521 tttatctttt ccttcatatt ttccagaaat taatcctgtt acaaatgcat accctacaga
    71581 ttcaaaatct aaatcttctt caacaaatat ctgtaaaatt tcttctggtg gtgttttttc
    71641 acttggtgaa aaaattctta aaattgtttg cataaaatca tttttattac caaccttaat
    71701 taaatttaaa attttatatg ttattccttc tattttattt ttagcattct tatcaatata
    71761 ataatcatgt atttcacaac ctgactcata aatctttctc aactttttat agtccatttt
    71821 atacactcct ctcttatagt tatttaatat tgttcttaat tgaacaacat tgaaacaaga
    71881 taaccctgat atgtttgatt tattattgct ttctaaatta ctcttaacta tattccttaa
    71941 agtgttatta attaaatgtt ttaagtcttt actttttaaa attaaatcta acacatttga
    72001 tttaagttta taatcatata tactttgtat ttttttattt tcttttacaa aaaactttgc
    72061 taaataagtt ggaatattaa aataatttat tttacatttt ttagaatcta tacttgcttt
    72121 aaattccaca aaaagtatat tacgaagttg ccattcactt tttaatgtat tttcttctac
    72181 tatacttttt ataagctgat taaaaggatt ttcatctttg gaatctgaat actcactttt
    72241 ttgagctttt aaactattat ttctttcaaa aagctcattt atacaagtat ccatgttaac
    72301 aaataaataa aactcatttt cttcattaat taaataattc tttcttgtat aagtagcccc
    72361 tgctggagta cagaacaata ttagcttaca aatatcacat attgatggta catacgcctg
    72421 attccaaaac atattcctag cattattggt gcttactcca agtggagcaa aattactttc
    72481 tgagtattca tctaatattc ccttatataa accacacatt tcacatactt ctaaactatc
    72541 catatacttt tttatatctt ctatgctttt atttttcttt ataaagtttt tatttatttc
    72601 cttcataatt ttttctatag tatttatact agacttacta actttttttt catttatatc
    72661 cttagctata ctatttaatc tatcatttaa atagtttttc aacttgtcat agtcattctc
    72721 ttctaataaa tcttctaact ctccaaaata tataatttgt cttaaataat cattaaacat
    72781 tacctgtttt aatccatcta aatctaattt cgccttagca acattaaaaa aactaacttg
    72841 tccaaaataa ttatcttgaa ctacatattt ataaagattt gctgtaagcc tgtcatttat
    72901 tgattttatt ttaaatatat ctatagtctc atctactaaa gcttctaaac tatcaaattc
    72961 atcataactt tttatcttag acatactatc tagcttctct tttatcatgt taaatttttc
    73021 ttcatcaaat tttttaatct tatcattttg ttgtttcaca ctatctttaa ttttcttaat
    73081 tccatctttg attctatcag gcttagattt aataaaattt atactgtaat caatattttt
    73141 tttaattctt tttgatacat catattcatc cataaagtaa ttaaaataat actcatgaaa
    73201 gttttcaaga aataagctat caaattcgat ataatcttcc ataataaata tttcagcttg
    73261 tttatctgct ttctttatta tatttaaaaa tccaactata cccatattaa aaaaccaatc
    73321 tcctctataa actcttaact tcatttatct cacctccatt ctaaaatatc tacatctcca
    73381 aatccctgag cacgtctaaa accaattcct agattgtaga taagatttaa atcatcaata
    73441 tcacctttca atataaattt acctttgtaa ccattaatat attgatactc tttacctgtt
    73501 atttttttaa attctcttaa actctcttta actactacct ttttgagacc tatattttca
    73561 aattctaact ttctttttaa tccattcccc ctataatttt taaccacttc attagttata
    73621 tagtttagtt cttctatgta tctatcatca gttatctcca agaattttcc ttctttagac
    73681 tttacacaga ttggagacaa tgcattaaat agtacccttt cttctgttac cttcttttct
    73741 cttgataaat cgattctaat tcttgtaagt tcgtagtctt tatataaaca ttttttagaa
    73801 gtcattaacc cattgtatat atgaaaaccc aattctaaat ctggagtact tatatttaat
    73861 attactttat cttctataat aaaattgtca ccttcaatac tatacttttt tatataaacc
    73921 gaaaatgtaa aatttttagt ttttttatta ttcttttctt tataatagta catgttttta
    73981 tagtaatctt cattagattt tcttattgct tcttttataa tactcataaa gagcgaatta
    74041 taagatattg gtatacaacc tgtactaaat tcaattttca ttttcataat atcacctctc
    74101 tcaattaata tattaacact agaccgtgaa gtatattggt ccaagtataa attatttgaa
    74161 aaaatcagta aatttttttc attttttcaa tctctaacaa tatatcattt ctaagtctat
    74221 ctggttctac aacctcaaca taagctccca tacttaaaat ccaacttttt atctcctcat
    74281 aacctctcat tttagcttta aacattatag atttatcatc atattctatg atttgttgat
    74341 tattaaccca aactttctcc tttataattg ttgaaaatgg atgactaatt tttataacaa
    74401 cattaatttc ttctccctta taaattccta tactattttt actatattca tcccaactaa
    74461 aacttttatc tacattatat ttttcttcta aaaccttata atctttaatc ctacataatt
    74521 taaagtctat aaacttaaat cttttttcac aaaaagctac catataagta tctgatttat
    74581 aattaaataa accatatgga tgaacaattc tttcactatt tcctgaattt aatgaataat
    74641 attctatcca aaatttgtgt tttgtggtat atgctctaat tatttcatta cacttatttt
    74701 tttcagactc ataatcacaa ttacgttgtg cttgaactgt aaaataatcc atatatgtat
    74761 cgcttttttc tcctgtattt aatactgctt ttattttttc tacaatatta ttaaattcat
    74821 ttttgtaaat atcattatta tactctaact gtgaatttat catatctaga attgaaactt
    74881 cactatctaa taatttaatt aatgagatgc tattacattt atctatttca taaccaccat
    74941 aaatcccagg tgttgaattt ataaatatac cagcttgctc taaatacatc ttgtaacttt
    75001 ttatttgcct ttcacttact tccaactctt ctgataattc tttgcatttc attttgcctc
    75061 tgctttgaat taatatcatc attctaagtg tattagctaa tttactaatg agtaaccccc
    75121 cctaataaca ttaattgata tttttattat ttatttctac acatatgtac atataacctc
    75181 tttatatttt atataagtag aattttttta taattttgac atttaccata ttatatttta
    75241 tatttgtaca aaattttaat agttaataca ataaaaaata ttactgaaaa aataaacatc
    75301 tataattcca gtaatattaa ttaattctaa acccttttta ttatcattta ataatagtaa
    75361 ctaaaaattg actaaattta accttatctc ttttaaataa ttatttctta caattaaaaa
    75421 ataaactatt tgaaccaaga acaagcttcc cattaccaaa acagctgatg atgctacttc
    75481 tatatcaaaa gaatttttca gtgcagtaag tgcaaatatt gaatgtataa cagccactac
    75541 ataaggaaac aaaaatacta ttccaatctc aattgttgaa gcttttttaa tctcattata
    75601 tgtcataccc aatttattta actgttcata ctttttctta tcaacttggc aatccatata
    75661 aaatttatta tataaaaaac tacttgtagt aacaaagaaa attaaaccta taaatattgc
    75721 taaaaatata aatgtagaat acattaattt cccagttcct atcattaatt ctttagagaa
    75781 aaacaaatat ggttgcatcc ctgactcatt attaaattta ttttcaaatt ctctgcatat
    75841 ctctatagtt ttacttgaat cctctacttc aaatgcagta aatctatcca ctataaattt
    75901 tgattctata ctatcataaa aattatcctt taccacatac atatttttgt aatacgctgg
    75961 cataacacat tcttccactt gttttgccac ctttaaacct ctattcccta caataatatt
    76021 ttctttgacg ttcttatttc ctggtaaact tgatgataat aatgatatac attcattatc
    76081 ttcaaaactt ataggcttaa cccctatagt ttctgtaatt tctaagtact tagattcttt
    76141 cataattctg acattaaaat cacttttctt gggaaataca gttttaaatt caccattaat
    76201 ccttgtatag tctacattct ctttttttag attattttct ataaacatca ttctttcctt
    76261 gtctttttct ttgctatcag gctttttagg gtcattatgc aatgtcattg ttgaataata
    76321 tattgtgtta ggatatatta aattaatttg acgctctaca tctttccaaa aggaataaac
    76381 tgtacctata gcagtaaatg ccactgcaga agttatagtt atcaagaaaa atattctagc
    76441 attatccttt accttataaa tcaagttcga aatccaaagc atatttgtat tttttctata
    76501 aaatctcttg tttaatttta gcttcttcat aaaaaataca ctaaattgag aaaagaacaa
    76561 gtaagttgct actatagtaa gggatgtaac tggaactaag cgcagatatg ttgtatattg
    76621 gtcggttgag gtaatagcat tataatacgc tattataaac aaacaaatac ttaatatagc
    76681 aagcattagt gatgttttag gctctggttt tggagttttt gtacctttaa gtaacttcaa
    76741 cacctgattt tctttaacta taaaagaagt aaatattgat gttaatacag ctaaaatcat
    76801 aaaatagatt aaagtagaca atatcgcatt aaatggtata tagaatttta atggtgataa
    76861 ttctaaaaaa gtagacataa ccactaaaaa tatctttgca aagattaagc ctattattat
    76921 tccaattaca gaagatacta tatttattat catattttct ataaagatta atttcattac
    76981 ttggcgcttt gatatacctg taatataaag tattccaaat tctttatatc tacttttaag
    77041 aaacacacta actgaataaa acacaaagaa gaataagaat agatatgcaa ttatcttact
    77101 catcttgaga ccttctttta aataatcagg aaattgagat acatctaaat ctggatgact
    77161 tgtaaacata ataaaagaaa atagcagtga agatgatata acaatacttg ctaagtatcc
    77221 taaatacgct ttaatatttc ttgaaatatt gttaatagca aactgtttta tattcattag
    77281 ttatcgcctc ccaataatgt aagtacatcc attatctctt gataaaactg ctctctacta
    77341 ctacccttat atatttcatt atatatagac ccatctttta tgaataagat tctactacag
    77401 tagctcgctg ctaatgggtc atgagttacc atcatagtag taactttatt ttctttattt
    77461 atcttttgaa atagctccat tacaaccttt gatgactttg aatctaaatt acctgttggt
    77521 tcatctgcta aaagtattga tggattgtta atcaatgctc tagcaatagc agtcctctga
    77581 gcttgaccac ctgatacttc aaaagttctt tttcctaata aatcttttat acctaatatt
    77641 gtagatactt cattcaatct ctcatcttgc tcttttacac tgaccttatc taatgtcaat
    77701 ggcaatacta tattttctcc tatagttaat gtatccaata aattaaaatc ctgaaataca
    77761 aaacctaact ctcttcttct aaataatgca agttcctcac ctcttaacaa taaaggattc
    77821 ttgcccttta attccatagt tcctgtagtt ggcttgtcaa tagtagaaat catatttaaa
    77881 agagtagttt ttccactacc agatggaccc ataattccta caaattctcc atcttctata
    77941 ctaaaattta tgtcatttaa tgcgttgaaa attatttttt taccatatac tttacttaaa
    78001 ttatttacag ttaaaatttt cattatttaa ctatccttcc taatttaatt tttgaaataa
    78061 catatatata attgttaatt ataatttatc ttagtttaat ttttatagat aataaatcta
    78121 ttttttctat acactaatta tgttagtaaa agctaatact gcaagtacta cacccatgat
    78181 tattctgtat acagcaaaca ctctcattgg ttttttcttt aaaaaattca cgaatttttc
    78241 cataactatt attgatacaa taaaagctac tataaaccca actaccaaag caatccacaa
    78301 tgttggtgtc attattgaat aatcaaattc aaataaatct tttcctgaag aagccaccat
    78361 agctggtact gctaaaaaga aagaaaactc agctgctgta ggagaattaa gtcctgctat
    78421 ccatccaccc ataatagtag atgcactcct tgacatacct ggccatgcag acaaaacttg
    78481 taatgtccca acctttaaag cttgtattgg agtaatttta tctatattat ctgtggaatg
    78541 attctttttt ctaaacaaag tttctactac taataataaa atcccaccca ctataaatcc
    78601 tataattaca gattgtaaat taaacaatga ttttatatta tcgtaaaata gaactcctat
    78661 aatcgcaaat ggtatacaac ctataattac attcattcca aatctaaaac caatctttcc
    78721 ttcttttcca ccagtaaata taaatttaaa aaattctata acgctatctt ttatcttttt
    78781 ccaataaagt actactactg ctaatatagc cccaagttgt attacaacct caaacatttc
    78841 agcaaattgc cccttaaaat ctattaaatt accaaccaaa atcatatgac ctgtagatga
    78901 aactggaata aattctgtaa gcccctctac tatagctatt atgactgatt tcaatataaa
    78961 aataacatct aaactcatca ataaacatcc ttcctttttt acaaaatttc tttttttatg
    79021 ttatttctct tatgttattt cttatattaa ttgtaattta gattagaaaa tttaactatc
    79081 tgcaaacatt acacaaacct tacatctatg taaggtttgc aaataaactc tcttaaattt
    79141 tgattattta actatgaata tttcatttaa taaattgtat aataaaaaag tacaattaac
    79201 taaaaaccct attgtaacag ataatttaca tctttatcaa actatttaca cctttattaa
    79261 agtagattga aacttctgta ccttttccta cttctgattt tatacttact ttatgaccta
    79321 aatcatcaca tacctttttt actagataaa gacccatacc tgtagattct ccaaacttac
    79381 gaccattttc tcctgtaaaa aatggggcaa ataccctttt taaatcttta gaaacaattc
    79441 caattccttc atctattata cttaaaatta catatttatt attttcttca gattttatag
    79501 ttaggtagtt actataatct ttagaatatt tcactccatt tgtaattaat tgacctatta
    79561 taaatttcat ccattttaca tctgtataaa cataagtaaa cttgtctact tgaatcttag
    79621 gaatcattcc actttttata aataaccttt tttcttcatt tacaatacta gatacaattt
    79681 catttaattt ggctttttca actataaagt ctttctgaaa attatctgcc cttgcaaaat
    79741 gcatagccat atctaaccct ttattcaatc tatctatttc ttttcttata ttttcagtta
    79801 cttcttctcc ctcataatct tggagctgaa ggcttataac tgatattggt gttttcatct
    79861 gatgaatcca ttggttgatg aaagttaaat gttcactatg tatcttttta tgctcttgta
    79921 tcattgattg atacaattta tgttgttgtt ttaaaataac tgatatattt ctccccaaga
    79981 atgagcttcc taaatacaaa aatgactcct ccaaagaatg tactcctttt tcaaacagat
    80041 tatatacttc tctattttta taatacttat atattaaaaa acataacaat ataaaagaac
    80101 taaataaaag aatatataaa ctctctccaa aactaataaa cttcataaga ttacagtaaa
    80161 taattgtcat aactatgcct atataatata taaaaatata acctttataa tcttttaaaa
    80221 ataacttcat actttctcct taccaattct tatttaattt atatcctaac cctcggacag
    80281 tctgaacagc attatctata cctaattcct caaatctttt ccttatcctg ctaacattta
    80341 catttaaagt attttcctct acaaactcaa tatcatccca tatcttttct aaaagataat
    80401 ctctacttac tacttttggg tatctcttga taagacattc aatcagtata ccttcttttt
    80461 tagttataat tagattctca ccatttaatt ctagttctaa cctttctgga taaaatttta
    80521 atccatctag ctccactatt ctttcgtcta ttttaggtga atattcacca tatacccttc
    80581 ttatatgact ctttattttt gccattacta catcatagta aaaaggtttt gttatataat
    80641 catctgctcc actttctaaa gctctaattt ggtctacatt accatctcta gctgatataa
    80701 aaattatagg taaattcgac tgttgcctaa tacatctaca ccagtagaaa ccatcaaatt
    80761 taggtaaatt tacatctaat agaactaagt gtggcttaaa actttcaaat gtaatcatta
    80821 tatcatcaaa gttctcttca acaatcactt caaatccata tttctcaata taatctttaa
    80881 gtaatgtact tattgatatg tcatcttcca caatatatac tttataactc atataattcc
    80941 tcctaatata cctatttact catcatataa cagtccaatt ttaatatatt ataatctttt
    81001 tacaatatat aaatttaatc atattactaa cttcacaatt actgacttca taagtataac
    81061 catattatta gacatcttaa taaaactcta taaaaaagta tctttttatg attaattaat
    81121 cattattatc aactaatcat tctaaaataa attgaaatta ctaattcaaa atagaaaaag
    81181 ttaccttaat aaggtaactt aaacttttat tatatttctt acttagtatt attttatcat
    81241 agtttctact tagcattata gattaattct tttatgttca cttttttctc ttgtaactta
    81301 ttttttatta agaagtcttg tgtatcttca agcgaagata tgtctttatc tgttatatct
    81361 aaactaaaat cataccaagc atataattct ttagtttctt caagacttaa atcaacttct
    81421 ttagctactt tttccatagc ttcatcaaaa ttattattta catattctaa agtttccttt
    81481 tcaaccttca taaatctttc aactatttct ggatgtttct ctgcaaagtc tgtgctaaca
    81541 gcagttacta ttataccatc tactaaacct tcaccatttg ctacaagttt actacctgac
    81601 ttcatagctt taagagctgc tggcccagca atcaatgcag catctacgct gccatcagaa
    81661 agagctgctg atgcctctgg aatacccata ttaacaaatt ccacatcatc catacttaat
    81721 ccttctttat caagtgctga aatcaatacc tgatgtaata tagtcccttt tgggcctgcc
    81781 accttcttac ctactaaatc tgctgctgat tttattgaat tattattagt caaaatcata
    81841 aatccttttg gtgacctgct ataagtgttt aatatcttta actctactcc attagatgca
    81901 gctattaaag ctgatgtacc acctagtgca tgtaagaagt ctatttctcc agcagctaaa
    81961 gcttgtgttt gctctggacc tgtagtaatt tcgtggaagt taacttttat attatccttt
    82021 ttaaattcct ttccaaataa atcatcctgt ttctgaatta tagaaggaac atttaatggt
    82081 gattttacat aagttaaatt tatttctgaa agtttttctc cattattttt tttagcacat
    82141 ccagctaaag atgctgttgc aagtgttaat atcgttatac ctatcaatag cttttttaat
    82201 ttcatttact ttactcctta aaatattatt aatttcttta aatgccttta tttgacttca
    82261 ttgtcattaa aatatctttt tttaatttaa tatagtaatc tgaagttaaa tctctattat
    82321 aaccatcttc tatattaaat tcttttactt ttctttcgtc tgtgaaaaca actattttct
    82381 tgcctatctt taacgcttca tcaatatcat gagttacaaa tatcacgcct ttattagttg
    82441 acttatgtat tcttataact tcattttgca tatcaatcct tgtaaaataa tctaatgccg
    82501 aaaaaggctc gtccataagc aacatatctg gattgaaaga aagagctcta gctatgctta
    82561 ctctctgcgc catacctcct gacaattcat ttggataaga gtttttaaat ttctgcaagt
    82621 tcatcatctt aagatatttt tctacatcaa tattactttt cctattatat ttatttgatt
    82681 tgttcaaatt atcatttgtt tctaagcttc taaaattatc atttataatt ttactcctaa
    82741 ttcttttatt gtgtttaaat tttctaagca ccttgtttaa aatagtattt tttctttgat
    82801 tatgaaaagc tatattttca cttacgttta accaaggcat taatctactc tcctgaaaaa
    82861 caaaaccaac attaggtcta tactctttat tatcattaat aaacctaatt tcaccactat
    82921 ttataccttc aagtgatgca attgctctaa gcaaggtagt ctttccacaa ccactttcac
    82981 ctaaaattac tgttatatgg tttctatcta tgtttaaaga gatatcacta agaactacat
    83041 gttctttatt gtcaacttta taacttttat ataaatttct aattatatat ccagagtcac
    83101 atactaaatt attcatatgc ttccaccatc tttcccttac ttaccttctt cacaataatc
    83161 gaaaatatgt aatctgtaat tattcctaaa agtccaatta cgattatccc tacaataaca
    83221 acatctgttc tagacatttc ttttccatca gatatcaagt atccaagacc agatgaagct
    83281 gctacaagtt ctgccccaat aattgctcta aagctatatc caagagctaa ctttaaccca
    83341 acagcaatat caagaatggc atttggtatt attattttaa atattatctg aagctttgaa
    83401 aactcaaata ctctacccac ttcaatcagt tttgagtcac aatttttaat ccctttcaat
    83461 gtgcttgtaa agattgggaa aaaagatgct aaaactataa ttataatttt tgattcctct
    83521 cctataccaa accataatat aagcattggt attaaagcaa ggggaggtgt attccttaaa
    83581 aaatttatta gagatttaaa gtattcgtat actccactat aaattccaaa gaatatccct
    83641 aaaggcacac ctatagcagt acttattgca aaacctatca aaactctttt catacttgca
    83701 tatacattaa taaatattga accatcactt atcatattta aaaatgtact atataccttt
    83761 tcaggtgatg gtaatatata atcactccaa ataccaagat aatttgtaat tttccataat
    83821 aaaagaatta taaaaaatat cagaacactc tttactagcc ttaaaaactt attattaaat
    83881 ttattgacca tttacaaacc atctccatta ttacaaccat ctttacaata aaacatttct
    83941 actatgtcat acttatcttc aaaaccattt cttatttttt gtatttcact taatatttcg
    84001 tcttcacttg atacttcaag aactttttca aaagtatctt tgaaatatcc taatggaatt
    84061 ggtgattcat ctatttttcc aagtgatttt acaccttctt gttcagcaaa ctctttccat
    84121 gtataaaaca tagcattacc cttatcctta aacttttcac ttgcaaagtc tcttaattgt
    84181 gtacatggac aggttactac caacatatca tctttacttt ttatatattt atcgtataaa
    84241 accctacaag tccttactaa tattggttct atcataggta cttcaaaatt atttatcaaa
    84301 ttatttcttt ttaaaagact tatactttct ggacatctac aatccaacat tgtattttca
    84361 gtcttttttg tatactcttt gtactgcttt tttacatatt ctaactgtgg ttcacattct
    84421 gttattatgt accccttttt tatcaaattt gctttaataa ggtgaaactt ttctccatac
    84481 attctttctg ccactggatt tattagaaga tatttattca taacgcctcc tattatttaa
    84541 tattgaatat tattatcaat taatagttta aatgagcact taaattttgt caatatatta
    84601 attttttctt aataaaacaa tttttatcta cattttttaa tattttaaat gaaatattaa
    84661 atagtatatg taatttattt taattaataa tgcatacatc ttactaataa tctatttata
    84721 taataatcta taaatattat ataataatac ttatataatt agtattatta tttaatagca
    84781 acagttattc acactaataa aagtagaaag tttttaaaat attatgaaac atcaaattta
    84841 attttaaaat tgcatcaaaa aatgaagtct caacagactt cactttttat aactcttata
    84901 aattattcaa aatatttata cttccctttc tacttcaaaa cgtaaaaccg tcttcaatga
    84961 ttcaggcgat acttttctag catcacttat ataaatttca cgatgtgtgt aagattttct
    85021 tataagttta ttttttttgc aaaattcgtt cataatgtca aagctttctg gttcattatc
    85081 atatgaacct atatgaagca tttgaacaca tagcccttca ttaatagacc caaatctaac
    85141 tttttcaaga agattatgag gttttttctt ctttactata tcagtagctt tatccaacag
    85201 gtcttgtgta acaaaatctg gctgacgtat cattatagtg taaattaaat cttctttaat
    85261 aagtacatct aagcctttac ttttttctcc cttatcccat ataccttcta aaggaaaaac
    85321 cgtatagtca aaataccctt ctggtacaat tcctttttta ggcatggttt ttattgcata
    85381 tgccaaagaa taaagtactc ctattgcttc agtaaattct ttagaatttg ggtctccact
    85441 cccttttaac atgataaatt tctgttctgg tatttcaact attacaggat tctttttagg
    85501 taaatataac tctttatctc tttttctcca ttcatatttc ataaaaatac ctccatatat
    85561 attttataca ttgatattat cacgctaaaa gtgacaacag tgtgtcataa ttataaatta
    85621 tattataaaa tcttcatcct ctaattcctc atacactcct ttttggtatc cattaccctt
    85681 agttgaaaaa aaatcatgtg ttttggtttc agtgctaaga ccatttaata caattggatt
    85741 tatagcatct acagtatata gcttctcaaa ccctagattt tctaaagctc tatttgcatt
    85801 gtattttaaa aagttcacaa cctctttttc aagcccactt tctctgtata taacatttgt
    85861 atactctatc tcattctcca taaggctata aagtatagaa tacatcttct cttctaacat
    85921 ctccttatcc attttatcaa atgaatcgta tatttcttgt gctagcaatc ctatatactt
    85981 accatgtaaa gactcatctc taagaataag cgatattatt tctccactag ctaccatctt
    86041 tccttgacca gatagataca atggataaaa gaatcctgaa taaaacaaga aactctctaa
    86101 aaatacactt gtaaccattg ataggtataa tccctcttga tttgtagtat tttcgtattg
    86161 agctaaaact aaatcagcct ttctttgtaa tgttggttct gtttctatcc attcaaataa
    86221 ttcatctatt tctaaattag aaagtagagt cataaatata gaagaataac tttttgcatg
    86281 tatttcttcc atagtcccca taaaagacaa gacagctttt ctttgtaggt tttctgttaa
    86341 actcatcata gatggtattc cattattccc ctgttttgta tccagtaatg ttaaaccacc
    86401 caatactttt ttatataatt ccttttcttt attacttagt tcattccata cctttatgtc
    86461 tttagaaaca gatatttctt caggaagcca aaattgtttt acattttgtt cccaaaaagc
    86521 ttgtgtaaat ccatcttctt ctctattcca gttaactgca ttatgtattt tatgtaaatt
    86581 aaatgtcata atttctctcc ctatactgaa cacactagac actcatgagt atctcttgtc
    86641 atctttgttc ttgtgtaata taaacttttc aaaccttttt tataggcata tatgtaatgt
    86701 ctagctatat ctctagttgt cttttcatct gtaacaaaca gtgttgttga tataccttgg
    86761 tcaacatggt tttgaactgt tgccactaaa tctatgacct ttctcatatc catcctgtat
    86821 gctgattgat aatacaacat attatcattt gttaaaaatg gcattggata tattgtagta
    86881 gagtctccat atgttcttac ttctactggc tcagtaattg gcattatact tgatgtagca
    86941 ttttgaacat aactaatgct ttgagttggt gctactgcca ttaaatatgc attatatatc
    87001 cctttttctt ttacctcatc caataatttt gtccagtctt cttttgttgg tatataaatc
    87061 ccttcaaata atgctcttac cttttctgac ttaggtagat aagattgttc atagtatttt
    87121 gataaaacct tgctatttct tcccttaaca tattctgatt tatcaaatcc ttcaaaagtt
    87181 tgatttcttt ctatagctat tttcatagaa gctttaattg aatagtatct aaccattgca
    87241 aaaaatacat tagaaaaatc tatagcatca tctgaagtat ataaaatatt ttctcttact
    87301 aagtatccat gtaaattcat agctcctaat cctatagaat gagacttgct gttactattg
    87361 cttaccgttg gcactggttt tatatctgtg ctatctgcta caaatgacag tgctcttatt
    87421 gctgtttcaa ctgtactttc tatagtttta ttatccatta cattagctat gtttaaagaa
    87481 cctaggttac aacttatatc ttgtccccat tcattttttc ctccataccc ctctatttct
    87541 gaaggtgttt gatattgaaa tatctcacaa cataagttag acattttaat aattccaaca
    87601 tcttttaatg tatgttctct attagctgta tctatgtaca ctacatatgg atatccactt
    87661 tcttgttgca tctgtgctat ttttgtcaac atctgcctag gatttatttc tttttttctt
    87721 atatcactat cattaaccaa tttatcatac cactcatcca tttctatctc atcaagagaa
    87781 actccatatt tcttatacac agtatgagga taaaatgcat atgctacttc atttttttct
    87841 gccaactcca taaatttatc tggtattata gctcctaatg ataatgtagc aagccttatt
    87901 ttttcatctg catttatctt tttagtatcc atcaacaatt caaaatcagg atggaataca
    87961 gtaaggtaca ccgcaccaga accttgtcta gctcccattt gattaaagta actaaatgat
    88021 tgttctaaca ttttagctac tccaacaaca cctccagctg caccttctat atctttgata
    88081 gattcgccag atgctctaag tttagataaa tttagtgcaa ctcctccacc aatctttgat
    88141 aagtgatttg atgaagatat agcatatgat ataccttctg tactatcttc tacagatagt
    88201 aaaaaacatg ataccatttc tccagccctt tttcttccag catttaaaaa agtaggtgta
    88261 gctggttgaa attcttgttt tactatttta tctgccaagt ttaaagctaa atctttatca
    88321 ccatttccta gtgtcaaagc tacaattaac actttatcat tatatgtttc taatatttcc
    88381 tttccatcat tgctctttaa tgcataattt tgataaaact tatttgcact catatatgat
    88441 tgaaattcaa acttatgttt atcaatcata tcatatatat ttttaataac atctatgcta
    88501 tactgattta ttacttcttt tgaataataa ccttcatcta ttaaatactt caatctttct
    88561 tctaggttat taaattttct caactttggc tgtataaatt cttctatgta actacttaag
    88621 gcttccttat ccttttccaa ttggtacttg ccttgttcat ttttaattat cacttgattg
    88681 ttaagctcta tccaattcat tatttaccgt ccttttaaca taatcttgtg ccataatttc
    88741 acttaatttg cttattattt cttttggtac ttatcagttg aaatcccaaa attaatctat
    88801 aattatagaa atgcaataag tatttttcaa aaacaaaagc actacatata gtcagtgctt
    88861 tatctattat atactacata tagtatatac acaaattttt ttatatctaa acttttatta
    88921 ctttataatt gacattattt ttatttaaag aaatttttat caactcaaat ataaaacaat
    88981 acaagcttat tatgtcctgt ttaaaaattt aaattatctc taaatagctt aaaaatatat
    89041 tctgatatat tgaaaaaagt actttcaaga tgatatttta tatattattt agaagttttt
    89101 tatttattgt tatataatta aatatatgac tattttgcta ctacatatat aaaaataaat
    89161 ttatgaaggt gattttaatg aaaactattt ctaaacaaga attagaaaaa acacttgaga
    89221 aacattattt atggcttaaa aacaataatg ctggagaaaa ggcagattta aaagaattaa
    89281 gattagaaaa tatggactta agaggatact gcttaagtaa tattgacttt tcgtggtctg
    89341 accttatcaa tttaaattta gaaaaagcag atttagaaaa ctctattttt aataattctt
    89401 atctttcgga ttgttcatta aaaaattcta tattaaaaaa tgcagactta agtggtgcaa
    89461 gttttagatt ttgtgactta tctaactcag atattagagg agctaattta gaaaattcaa
    89521 atttagaaca tgctacttta aatggagtaa tatcagatga aagtacccgt ttttttaaat
    89581 tgtattgtcc tgaaacagga gcatttatag gctataaaaa atgttttaat tttagaatgg
    89641 tacaattatt aatacccagc gacgcaaaaa gagtgtctgc tacatcaaac gcttgtagat
    89701 gtgataaggc aaaagtactg tcaattacta gtatagatgg taaagaatct tttaaaagtg
    89761 ctcgtgcata tgctgatgaa aattttatat atagagtagg agaaacgatt gtagctgaag
    89821 attttaatga aaatagatgg aaagaatcga ctacagggat tcactttttt ttaactagag
    89881 aggaagctat tggttatcta taaaatgcta aagttttata aatatataaa tacataaaca
    89941 aaataacaaa agagtataga ttgtaatttc aaaaaaatta taatctatac tcttttttct
    90001 atagcacata agtacatttt atttcataaa ttataataaa ttatttatat ctgcactatt
    90061 gattatttga gaaactttat ctatatgttc tatcttacta tccaaactgt cataatactt
    90121 cttactctca tctctatttc taaaagaata aactttttta tccaatttta aagcctcttt
    90181 tataatatct atattcctct tatttgtctc tcctacacta aatcctgtat ctattataat
    90241 cttggaatca tttaaatttc taatagccaa attgaaactc tcatcactta taggctcaaa
    90301 tggattttct gtaaacatct ttatccccat agttctacca atttcataat caatatcatt
    90361 ttcatgtatt attccagaat acagacctat acctttttta gtaaaagctc tataaattgg
    90421 tgttgcttta cccccaccac caattataaa aacttcattt ttagatttat tactaatttc
    90481 tactgaacct aaaagactat taaagttttt atcatccaat tcataaagcg aatttataat
    90541 atcctcatct actacatctt caggctgacc ataagctatg actttattat tttttataag
    90601 agcaacttta tcacaacttt ttaatgctat gtctatttca tgcaatgaaa gtattactga
    90661 tattgacttt tctttactta attttttcag tatgtttatg agttccaatc tatgttttat
    90721 atccaagtga gttgtaggct catctaatat aattatttct ggttcttgaa caagagccct
    90781 tgcaaccaac accttttgtt tttctccatc acttagttca tcaaaatatc ttttctttaa
    90841 atgtaaggca tctacagact ccaatgcttc atctaccatt attaaatctt tcttagatag
    90901 actcccaaaa aatcctgtat atggatacct tcctatattg actatatctt gtacagtcat
    90961 taaatcacct aataatctat ttgtaagtac taaagccatt ttttttgata atgcttttct
    91021 tgatatacag tttatgttta caccattcaa atatatttca ccttttattg gtgatataag
    91081 tttagataag gaccttaata gggtagtttt tccagctcca ttacttccca aaagacatag
    91141 tatttctcca ttttttactt ctacatttat attacttata acaactttgt tattgtatcc
    91201 tacacttaaa ttattagttt tcaacatgtc taagccctat ccttcctttt caccaataac
    91261 attactacaa ttggagctcc taataaagat gttatcgtac ttattggtaa ttctactggt
    91321 gataaaatta ctcttgatat taaatcacaa agagctgtaa ctatactgcc taaaaatatt
    91381 actactggta tcaaaactct attatctgat gttttaaata tcatttttga aatttgtggc
    91441 actgcaagtc caataaaccc tataggtcct gcaaatgctg atactattgc tgctaatata
    91501 ctagatataa atactatcaa aactctaaaa aatcttatat ttatgccaat actttttgca
    91561 tactcttctc ctagtaaaaa agcatttaaa ggttttatta tgaatacagt catcaaaaga
    91621 cctaatatac caactattat aagtatttct accttttccc atgtaaatcc agaaaaactt
    91681 cccatagacc acatagtaaa ttcttttaat ttatcactat ttgcaaatga ttgtaacaca
    91741 tttgttatac cactacatac atatccggtc ataattccta ttattaaaag agttgttata
    91801 ttctttacct tatttgcaaa taaaatgact atagacatta caattaaagc tcctataaaa
    91861 gctgctacaa atatactcat tgaatttaca ctctcaaatc ccaatgtgta tcctcccaaa
    91921 atcatgagag ctactgataa agtagaacct gaagatactc ctaatatata cggttcaact
    91981 attgggtttt taaaaaatat ctgtaataaa atacctgata ctgctaggca tgctccacct
    92041 accatggttg caagtactct agggattctt atattgtata ttattttata atgttctgta
    92101 ttatccatac ctcttaatac aacatctatt acttctttaa attcaatttt tacagagcca
    92161 aatgctacac acactaaaaa agtaagtatc atacttataa ttaatactat aaatattgtt
    92221 gtattcctta ttctcatttt attgataaaa tccatttgta tactcctctt agcaaagcta
    92281 tattaactaa aatctataaa acctatcttt ttgtgtcctc tctgtactat ttatcataat
    92341 ttatataatg tttgattttt tcacctgtag tacctgggtg gaacatctca actaaatcta
    92401 ctattaattc gtccgtctta tctactgatt gataataatc tggagcaaac acccataggt
    92461 tcccgttttt tacaacatcc aaatcagcca atacaggtgc actagatttt atagaatcta
    92521 aatttggtgc ataatctgaa cttgatgagt atacaaatat atctgcatct tttgcaactt
    92581 catgcaattg ttcaatactt actcgccctt catttgaatc tattgaagca catgcattta
    92641 ttccaccagc catttttatc atattatcca catatgaatc tttaggagct atataagcac
    92701 ttccttcata tacagatgcc catacaactt taggtttagc tttgtttttt atttttttac
    92761 ttacttcttc taccttttgt acagttttat ttagttcact agtagccata tcttctttat
    92821 cataaaatgc agctattaaa cttgtccact ccattcttcc aaatggattt tgctctttat
    92881 actcattgtc tacaacataa ttaagcccta attcttttaa tttgtcaata aattttgtat
    92941 cagcctctga taaaccacta ctcactatag ttaagtcagg atttaagtct actatttttt
    93001 cataatctgg tgctaaattt gaaccaacgt aatgtatttt tccatctttc attaaatctt
    93061 taatttcttt aatagtccag tactgctctt ctgttgttac ggcttttata ctatctagtt
    93121 catctattgt tctcagagaa caagcctgtg ttgtagatgc tattaataca ttgtccacag
    93181 gcgttctaac tatattagca tttttatatt tttcaggaat tttaatcttc tttgggatta
    93241 aaatcagttc tcgattatct ccatcagtta cttttttaat tccatcttct aaatcttcaa
    93301 cactaaatcc actagcatat tttaattcta atttcttacc agtactagct tgtttattct
    93361 ctttgtcact tttactacac cctataagac ctcctaccat aaaaattacc atagcaatta
    93421 aaaaaaactg ctttaaaaca tttgatttaa atttcatctc tttcactcca atatcttatt
    93481 ttttatgagt ggctaaaaaa ctttaataat atatgctttt taaagtatac ttattaaagt
    93541 tatcctaaag tcatctttaa taagtataac cttttaataa aataaaaaac ttctcctaat
    93601 ttaataagga gaagttcata tatcaacttt acaaaaacga attgatactt cattttatta
    93661 taaattacaa tctccaccct aaattgaact tatataatac tgggcaggtc tcctgactca
    93721 cggcttttcc tccatcaccc ttcccaaaat ttttataaat ttcagtggat tttgatttcg
    93781 tatccgtata cagtagcggg ggctgtagtg aattttcatc actttcccta ttaaactaat
    93841 atagtaccct agtattattt taatttaatt tttattaaga taattgtaat ttattgttga
    93901 cctctttgca aggttgattt aacatatatt taattcattc atcaattttt taatttcaca
    93961 tacttgtttt tcatttggtt ttacaaatgg agcagtcata taatcactta tttgtaaccc
    94021 tctgatattc atagcttttt tcattgctgg tataaatgga ttggttatta agtaaaaatc
    94081 cattgcttta ttaatatatt tttgaatttc tataaccttc tccatatctc tattattgta
    94141 agcttcagcc catttggagc aaacttctgg aattaaattt gataaccccc ctatacaacc
    94201 tgctccccca gacattagat tatgtataaa gttttcatca taaccactat aaaccttaaa
    94261 ttctgggaac tctattttca tagtatttat taaatctctg gtatgtccca tcaaagttac
    94321 tgtatcctta tatccaacta tgtttttatg ttttcttgca agtcttaatg taagttctgg
    94381 acttaaatca taaccagttc tatctggata attgtacaaa tatatttcag cctctgtatt
    94441 cttagctatt tcactataaa ataactcaat actatcatca tcaagagaga aataataagg
    94501 gcttattatc ataacaccat ctgctccact attatgtgca tagtttgaaa gttctataga
    94561 ttcatttata cacatacgac ttgtacctat aaacactttt actctcttat ttacatgact
    94621 agttactaaa tctataagtt ctttttgcat atccatagac atattaaaga actctccagt
    94681 acttcccatt atgactagtc catctacacc tccatctata agatggtcat acactttttt
    94741 attaccttct ttatctaaat ttccttcttc atcaaatatt gtaacaactg gtgtcaaaaa
    94801 ttctgctttc ataatttaaa ctcctaaaat ttattttaat atgttcatta atacatcaga
    94861 tttagcttta atattaatta tattaaaaca gttacttaat gtaatttttt aaaattccaa
    94921 tactctcaat ctcacattca actacatctc cactactcat atatcgagga ggtgaaaatc
    94981 ccatccctac tccagaaggt gttccagtag ctataatgtc tccagcctct aaggtcatac
    95041 cttttgaaag ttctgaaact atgtctgaaa cgtctgatat gaataatcct gtattagaac
    95101 tttgtctttc ttcaccattt accctactac atactttaag ttttatagga aaaggaactg
    95161 cacttttatg taaaatacat ggacctaaag aggtaaatgt atctaatcct tttcctctaa
    95221 accattgaac atgttctttt tgcaactttc tagcagaaat atcatttata acagtgtaac
    95281 caaagatata atcttctacc tcttcttttc taatattaac acctgtttta cctattacta
    95341 ctccaagttc aacttcatag tctagttgat tatttatctc aaaatgacca tctatctcat
    95401 cttctggacc tattgccttt gtcacacgtt ttgtaaaata aaccgttttt tgtggttctt
    95461 caaaactatc atcaaagtga gtttgagttt cttcaagatg gtctttatag ttcactccca
    95521 cacataagat atcatgtatt ggtttttcta taggaacatg taccgtacat ctctctcttt
    95581 taaaaacatc ataattatag aatgtacttt catttataat agttttcaac ttaataatat
    95641 cttcactaga tacattttga attaattcat ttaaatcctt aaaatttcta ctgagattta
    95701 aagagttaac atctataata aataattcat ctttagaaaa aactccagtt cttttacatt
    95761 cattttctcc ttcagtaaaa cttacaaact tcataatctc accctttatt aaaattctat
    95821 gtatttattt tcttacttaa agattataca tagttgcata ctctagctat tgccatttca
    95881 gtatatccaa gggactctgc ttttgattgt tttccatttg ctacatctac aaccatattt
    95941 aacaaatcat ctgttaattc ttccattgtt tgtggaccat agataactgg actagcatca
    96001 aaatccatat tatcttccat atttgcaaaa gttattttat ttccagttat ttttattact
    96061 ggtgctattg gatttcctga tggtgttcct cttcctgtac taaataccac tatttgtgct
    96121 cctcctgcta ccataccagc aacagatgaa gcatcatttc caggggtatc cattataaca
    96181 agacctttct tatcaatttg tttggcataa tcatatactt cacttatttg tctatgacca
    96241 cctttatgta tacaaccaag tgatttttct tctaaacttg ttaaaccacc agctatattt
    96301 ccaggagatg gattaccttc tctaacttct tctccaacca attttaatga attttcatat
    96361 ctatgtacta tatgtaatat tttttctttt acttcttcat ttactgctct cctagctagt
    96421 atatgctctg cacctataaa ttcagtagtt tcacttaaaa ttgatgttgc tcctaaatcg
    96481 acaagtttat cacttaattc acctattaat ggatttgctg ctaatccact tgttgggtca
    96541 gacccaccac attcagttcc tagtataagt tcagatattg gaaattcttc ttttctaagc
    96601 aaagaagctt cttgtgccat ttctctagca taacgaacag ctttttctat agtttttaaa
    96661 gttccatgtt cttcttgtat tacaagagtt tttattggct tatttgttct ctcttttatt
    96721 gcatcaacta ccaaatcatt ttggcaattt tcgcatccca aagatacaac tattacacca
    96781 tatacatttg gatttgctgc ataaccagcc ataacatcca tagttaattg ttggtctgaa
    96841 tgtacttgtg aacatccatt ctgattatta aaggtaactg ccccttgaac ttgtgatgct
    96901 attattctag ttgtatctga tgcacacaca ctcgtaggca atattaatat gtggtttctt
    96961 ataccaactc ttccatctgg tcttttatat ccataaaatt tcatactgat ttcctcctaa
    97021 aatagtctat atactttcta taatttttat atattataat ttttattaca ctcactaatc
    97081 aaacagctaa aaacctattt aaaaactatt tgacactaaa agcaatgaat acaactttta
    97141 taactctata aatattattt tatatattag tcataacttg tttatccaaa tcctctctaa
    97201 cacttctaac attatgaata tgtacatgtt ggccactttt tatttcctga tttgcttctc
    97261 ctatatgttc accatattta gtaactttat cacctttggc catatctttt atagccaatt
    97321 tatgataaat agttatatca tctagtgctg taatcgtttt tactgttttg tcttttaatt
    97381 taaaactaat ttcactacct ttagctattg cttcaatagc tactgcaaca ttatcttttt
    97441 catcaattat cactgcattt atcattattc ttgctctcct ttataaataa tataatttta
    97501 ttaatatcct aaaatctaat taaagaattt attattttac caacaactca ctgtaatata
    97561 aattaagctg ttattgtacc atcctcattt tgttttggga acttaggaca tccatacttc
    97621 ttagcccacc aactagttat aagaggtact aatattgcag tgacaactac tgcggctgcc
    97681 acttgtgttg tagctgttgc aacatatggt tgccatgatg ggtcaactaa tgcaacagca
    97741 gcaggtacag caattgcatt tccagcagtt gtagctacag cccatcctgc atatcctgga
    97801 cgttttccta taaatttatc acagaatact ataaatatac ctcctacaaa tacagatatt
    97861 aatccaagta atactcctga taaacctcct tttagtatac ttgcaagatt tatgctacta
    97921 ccaagtgcaa accctacaaa aggtacgagt aaattacacc ctggctctaa aaactctttc
    97981 atttttttgt caaaatttcc tattatcata ccaacaagta ttggcactat tgcagccaat
    98041 aatgacaaca atggtacatt tgcaagacct gaagtcccaa gagctattaa tgtaaaaaat
    98101 ggtccatcat ttaaagtaag taaactcata gttgcacaat ctgtttcatc tccatatgaa
    98161 gtcatcaatg aaagatatat actaccatta ctatttgtaa ctgcacttat aacagccaat
    98221 gttgtcagac caaggacacc atccattcca aatagctttc ctataagtat acctatcaaa
    98281 gcacctatag caaatttaga accaagaagt ataccacctc tctttaaaac ttttggcatt
    98341 tctctaaatt gaagctgagt tcccaagcat accagttgaa ccccaattat agttgcagac
    98401 ccaacacttg aaaatactgc tgtcgtaaac gaacctattt gtacaatatc tgggcaaaag
    98461 gtattaatga aagctgctat aaacattggc actatcatca tacctgcagg aattttttta
    98521 ataaaatcca ttatcataaa gttaatcctc ctaaaacatg atatttaatt actcttatta
    98581 agtttttagc tttaattcta acggccgttt cggttcgtat ttaagaacta agtttttaat
    98641 tacgaatctt tgaattaatg atagcatttt cctatttatg agtcaataaa aaaaataaat
    98701 ttttgtcaaa attttttcaa actttctgat aataccttgt atattatcaa atgacttcct
    98761 tatatgcaaa aatacagcgt atttaaaatt atatagtatt caaaactata aagtttttat
    98821 acagtgtaaa aaatagataa aatataagtt cataatattt tgatatttac atataaaaat
    98881 aactattgca tatatttact taaattatat aactaaatat cacttacttt ctttaggtat
    98941 attttggaaa ttagttattc cttaatgtac agtctaaaaa aatatctatc tgtatctaaa
    99001 tcaagagctt atttatatat aaatttacat atttattttc tacattgtaa aatatcgtat
    99061 agtgtgctaa aatcaacata ttctaatact ttaaattaat aaaaagaggg ggaaatttat
    99121 gacttcagat aatcatcgcc caacagctag gatattagat attttagaag cattagcaga
    99181 atccgaaaat ggatatactc ttacagaaat agcagatgtt atcaatgctc caaaaagtag
    99241 catctttcct atagttcata cacttaatgc tagaaaatac attactattg ataaaaacac
    99301 atccaaatat tctataggga tacgttctta cacattaggg tcttcatttt ttgaaaaaag
    99361 aactattttt aactatataa ttgacgaaat gactaatata gttaattcct gttctgaaac
    99421 ttgtcagctt ggtatacttg acaataatga agttctttat attggaaaag tagattctcc
    99481 agaaccgatt agacttattt cttatgttgg aaaaaaaata cctgctaatt gtacaggtct
    99541 tggaaaagct cttttatgtg attttgacaa ggaaaaacta ataagcttat atccagatgg
    99601 tctaaaaggt tttacagaaa aatcaattac agattttgat gttttatacg accagcttct
    99661 tgaagttcaa agaacacaaa tagcttcaga gtgtgaggaa tcaatggaac acctaaaatg
    99721 tcttgccgta ccaataagaa aaaatggatg tataagagct gctgttagtg taacactgcc
    99781 tttatttaga gctaatgacg aaaaaataga actgataaaa aaattactct taaatgctca
    99841 gaaaaatatt caatcaatga tggaagaacg taatgaagac attgtaataa attagattaa
    99901 tattctgtat atggaagata tcaaaacata ataattaaaa attttgtttt aaactaatgt
    99961 atttatttga tttatactat aaaaacaaag ttgctgattt aactacagta actttgtttt
   100021 ttttataata ttttttatat ttttattgac atgataaaaa tactatgtta aattctaatc
   100081 ataatcgtaa ttacgaatta agttcttata taagaaccag caaccctatc tcaatctaag
   100141 atacgatata ttatgagtaa attagtgaaa attgttagat atatattttt acataaaaat
   100201 caatgaaaat aaaaggagat tttagttatg agcatttatt atgtaccacc aataaattta
   100261 cttgggaaag gatgtcttaa tgaagctaaa gattcaatta aatcattagg aactaaaaaa
   100321 gcatttattg ttagtgataa atttctagtt tcaaatggta ctgtaaaaaa agtaacagat
   100381 attttatctc agatagatat agactttgtt gtttatgatg aagttaaaca aaatccaact
   100441 gtaactaatg tgaataaagg acttaaaata ttaaaatcag aaaattgcga cttcgttata
   100501 actataggag gtggttctcc acaagactgt ggtaaagcta tctctatatt agccactaat
   100561 ggaggagata ttagagacta tgaaggtatc aacaaaactt ctaaaaaatg tcttcctatc
   100621 atagctataa ctactactgc tggtacatct gctgaagtta caataaacta tgttataact
   100681 gatgaagaca gacatgtaaa gatgataatg gttgatacca attcactagc cacaatgaca
   100741 gtaaatgacc cagaattaat gattagcaaa ccatctaatt taacagcagc aactggtatg
   100801 gatgcactaa ctcatgctat agaagctgta gtagctaatg gtgcttatga tgttacagat
   100861 tcaactgcct tatatgctat aaagcagata tttaaatacc ttccaagagc tgttaaaaat
   100921 ggtaatgaca tagaagctag agaacaaatg tgttatgctt gttttttaaa tggaattgcc
   100981 tttagtaatg caggtcttgg aaatgtacat gctatggctc atcaattagg aggtttatat
   101041 gatttacctc atggagtatg taatgccatg ctacttccaa tagttgagga ggaaaatgct
   101101 aaatcagccc ctgccaaatt ccgaccaata gctgaagtta ttggaatgga tgtaaataat
   101161 aaatcagata aagagtgtgt tgactttgta atagataaga taaaagctct ttctgaagag
   101221 gttggaatcc caaaatcttt aaaagatgta ggtgttgata atcctaattt tgaattgctt
   101281 gcagaaaact ctatgaaaga tgcttgtgct ggtgctaatc ctgttttctt tgataaagat
   101341 aagattatag aattgtttat gaaaatatct taattatgta tatttataag agagtgcctc
   101401 aaaatatctt tgttttgtta atagcactct cttattacac tttttaaata gtactcttta
   101461 tgagaagtta atatataatc ctattaattt aagaatcatt atcccaataa aaaactcaaa
   101521 atttttctta atgaattata actgttgttg tatctggtat attatcatat atccactttg
   101581 cattttccgt acttaatctt atacatccat gactaagagc ttcaccaagt ctgccatcta
   101641 ttatataact tcctgttgaa tcatatagta cagaatgata gtaatatcca cctttaattc
   101701 tagtagcata cttaactgaa tactcatccg ttccaaaaga aggttttctt ccacttatat
   101761 aaaatatacc tgttatagtt ggagtctcag gcttgcctat agtacattgc cacttgtaaa
   101821 gttgtcccca tcctccatta tctttcttaa aaatataagt atatttattt ctaaggtctg
   101881 ttacaattag ataatcgcta gtacttttaa tattattttc gttaacaaat ttaatcatgt
   101941 cagtataaaa atacttataa tctaattcat ttggtttatt cccatcaatt gataaaaaat
   102001 aattaaaaac atatcctatt ttatcatcat atacaacctt actccaatct ccatctactt
   102061 gaaggacttc aaccctagat ttttcaggaa ttactgtaat tatattggaa gttgtagatt
   102121 tatcttctct taagtttaag ttactccaag catattcgct tactgatacc aactccttat
   102181 acacatatcc ttcttgagaa ttgtacatca ccttaatcca gtcatcttcc tcatccaata
   102241 cttccatttt ggctccctga ggtattactg ttatgatact tgaattggta gatttagcag
   102301 acctcaaatt tatatttgct aaagcatatt tatatatagg tgctctatat tttttaatat
   102361 ttagtttagt taatttcaca catatccatc ctctcatatg tctttttaca acattttatg
   102421 aaattcgata tgtattcgtt ctcctatttt caaactttac ttgtattact aagaatattg
   102481 ctacatatac ttatatattc tcacctatat ttatctattt atttaaattt atgatattac
   102541 attctttaat atactcttca attatacctc tagccacatt aaaataactt atacacttgt
   102601 caactatgac tgcatcacta ctaactactc tatctaattt ttccaataca acatctgcat
   102661 tttttactaa ttctacttca gtttcaattc cccaagtttt ggcatgctct aatatcctat
   102721 actttaaatt tcctgaatta gaaactggag catctaaata aaatttaaga ttctgtgcat
   102781 tgtatttatt aaaaaattta cctatcagat tcaaagcttc ttctgtttta tctataatct
   102841 tataggttcc cctaagacct gctaaatctc ttatattgcc atcacttcct attactagag
   102901 taccaccaga taatcctacc tctatggata ttatcaaatt aaagccatct atatttacgg
   102961 tatcttcttt gattttatct agagaaagcc tctttgattg tcttataata tttttttcat
   103021 cagtacagac tgcccttttt aatgcatctc tttgtcttat agaaaactga tatctatcac
   103081 caacaaaagt aacgactggg tctattttgt aatccctatt taataaccat tctatttctt
   103141 ctttagcctt tataagtctt gttaattctt tacttgaaaa ccatctttta tcactatcat
   103201 caaaccctct tttagatatc ttatttatat catttcacca ccttgcttta aagatataac
   103261 tgtactacat taaatacaga ttatttcacc ttttaagata atttttaata ccattaaaat
   103321 atatttttat tatttaaaca gtaaaaaaga gacttatctc tattttttga gatagcctct
   103381 ttctaaaatc tacttataat gataatttta gatttatata ttaaacttat gttatacttc
   103441 ttttatcata ttaggagata gaatatcatt aagagcttct gtcatagttg gatgtgcata
   103501 aacacggtct cttaaatatg tatactctac ttctaaatct acagctaatt gaataagatg
   103561 gataatctca cttgaatttt cacatatcat actagctcca agtatcttat tggatttttt
   103621 atctatcact atttttataa acccatctgc cttgccaatc tgtttagctc ttggaattgc
   103681 ttctactggc atctttgcca ctaatacttc atatcccttt tctttagctt gcttaacatt
   103741 aagaccaact cttgaaaatg ctggacttat aaatatagaa tttgggatat tctttctatc
   103801 attagttgtt ctagtcttat ctccaaataa ctgattaata actatgcgat aatcatccaa
   103861 tgaaatataa gtaaattgtg gacctccatt tatatctcct atagcccata tatgctcttt
   103921 attagtctta agagtttctg agactttaat aaatcctctt tcatttaatt ctatacctgc
   103981 attttctagc cctagccctt cagtgtttgc ttttcttcct gtagctacaa gtatcatatt
   104041 acttgttaat tctttagatt taccatctac ttcatattct actattgcta attcagaaac
   104101 ttctttaatc tcttttatat ttgcattatt aacaattttt acatttctct ttgcaagtaa
   104161 ttttattatt tcttctgaat cctcaacatc ttcatttggt aaaatgccat tattagagtt
   104221 taatattgta acttctgctc caaatgaact ataaatacca gcaaattcta aacctataaa
   104281 tccagctcct ataattgtca tttttttagg taaaactctt agattcataa gagactcact
   104341 atcatatact atattcttat tttcaattcc cttaatatta ggtataaatg gtcttgaacc
   104401 agtattaata aatatattat ctgctactaa ctcataaatt tcattttcag ttttgacttg
   104461 aactgttttt tcatcaatga aagttcccat tcctgtgaat atagttacat tctcatttga
   104521 atttaattta ttatagttag cttctctgag ttttgtaatt aaagtttctt ttttatctat
   104581 agctttctca tattcagctt gtacttcatc ccaagaattt atattctttg tttttacgct
   104641 gtttgctgaa ttttctaagc tttttgttgg tatacatgcc acatttacac atgttccacc
   104701 atacatcttg tttgactttt ctattaaagc tacttttaat cccctgtttg ccaaatcccc
   104761 tgctaatgtt tttccacctt ttccaaatcc tattattata gcatcaaatg tttttttcat
   104821 ataaaattca accctttctt gtattgttta tacttttact ataacaagat tatttaggtg
   104881 gtacaattag gcactttttt gtaaccttaa attgatttat aaacttatac ttaaatactt
   104941 ttatttattt ctctgatttt caataaatga tgccatagtt gccttatcca tagctcctgg
   105001 aatatactga gttacatatc cttccttgtc aattataaat gtagttggaa aagcattaat
   105061 tccatattga tatgctaatg ctccatcttc atcaagtaca acagggaatg tataaccttg
   105121 ttcttttaag aagttaacta catgttccct tgaaccttct ctacctaaat taggtgaagc
   105181 tactcctaaa attactacat catcattatt tttattatag tctttataca gttgttctat
   105241 ataaggcatt tcttctttac atggaggaca ccatgtagcc cagaaattta agaatacaac
   105301 tttgccttca taatcactta atttatgtgt tttaccatac tggtctgtta aagtaaaatc
   105361 aatagacttt attctatctt catcattaga ccctttatca ttattgtcat tattagagtc
   105421 tttttgacta tcattaccat cagaattttc ttctttatca gtagaatttt ctcgcttatt
   105481 ttcttcttgc ttgctttcaa ttttactatt ttgtgacgta ttaaagtgtt tgcttatact
   105541 tccaaatcca tttaccatca ttaatattcc tgatactata agtataatcc cacctatttt
   105601 tttaataatt tccatgttag attttatctt atctatagtt ttaaatagtt tactgtaaaa
   105661 catagctgta attatgaatg gcaaaataaa accaatagta tatactgcta ttaaaagatt
   105721 ggcactaaga tgattacttg aacttgaaac catgactaat acagatgcca atattggacc
   105781 aatacaaggt gtccatccaa aactaaacgt aaaacctaat ataaaagctg ttattgcttt
   105841 catttcttta aacttaacat taaatctttt ttctctattc aatattgaag atttaattat
   105901 tcccatgtaa aataatccca tgataataat tataatccca ccaataaaca ttattaaatc
   105961 tttattttca ttaaaaaaca tactcaatac ttttactgat gaacccaata taaagaatgt
   106021 tgttgagatt ccaagagcaa aaaatatagt atttttaaat aaagaacttc ctataaaact
   106081 cgtctttcct tcttttaaat tttctacact actatttgac aatatactca aataaatagg
   106141 caaaattggc agtatacatg gtgaaaagaa tgatactata ccttctataa atactaaaaa
   106201 taaatttaca ttttgcaaat taaaattcct ccttatttta tttccaatat tacaatccca
   106261 tttctaaatt aaaatctaac taaaaaatta tgtaatttta atacattttt ctactcatag
   106321 cttaattaaa ttttcattac ttacagagta aattttagta tttatcacaa tttaaatttt
   106381 tattgctcca cataatcatt ttttccatga caggaagcaa tcgtaatcct gattcactta
   106441 aaaaatatgt tgactctaca acattattaa ccaacttttt ttggttgact ataatcccct
   106501 gttcaattaa aaaatctagt tgttgaacta acattttctt actacagtct ggtatagctc
   106561 tttgtagctc tccaaatcgt tttttatctt cttgtatttt ccatataatt atagccaccc
   106621 attttttacc taaaatattt gataaagctt ctactggaca attatatatc atatcatcta
   106681 tgttgtacat tttattctcc tcttttcttt atttaattta ttaaacaaac ttttatagca
   106741 atatatttat taatattata aatataccta gctttat
//
LOCUS       DAMJWU010000004       102652 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMJWU010000004 DAMJWU010000000
VERSION     DAMJWU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 102652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.004"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            32..1312
                     /locus_tag="PC460_000342"
     CDS             32..1312
                     /locus_tag="PC460_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF2599251.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1575..2966)
                     /locus_tag="PC460_000343"
     CDS             complement(1575..2966)
                     /locus_tag="PC460_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2599252.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2977..4197)
                     /locus_tag="PC460_000344"
     CDS             complement(2977..4197)
                     /locus_tag="PC460_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF2599253.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4211..4999)
                     /locus_tag="PC460_000345"
     CDS             complement(4211..4999)
                     /locus_tag="PC460_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2599254.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5016..6290)
                     /gene="larA"
                     /locus_tag="PC460_000346"
     CDS             complement(5016..6290)
                     /gene="larA"
                     /locus_tag="PC460_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF2599255.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6408..7121)
                     /locus_tag="PC460_000347"
     CDS             complement(6408..7121)
                     /locus_tag="PC460_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2599256.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7246..8505
                     /locus_tag="PC460_000348"
     CDS             7246..8505
                     /locus_tag="PC460_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF2599257.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            8785..9351
                     /locus_tag="PC460_000349"
     CDS             8785..9351
                     /locus_tag="PC460_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2599258.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9552..10757)
                     /locus_tag="PC460_000350"
     CDS             complement(9552..10757)
                     /locus_tag="PC460_000350"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2599259.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10860..10966)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11058..11426)
                     /locus_tag="PC460_000351"
     CDS             complement(11058..11426)
                     /locus_tag="PC460_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF2599260.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11478..12755)
                     /gene="obgE"
                     /locus_tag="PC460_000352"
     CDS             complement(11478..12755)
                     /gene="obgE"
                     /locus_tag="PC460_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF2599261.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12866..13156)
                     /gene="rpmA"
                     /locus_tag="PC460_000353"
     CDS             complement(12866..13156)
                     /gene="rpmA"
                     /locus_tag="PC460_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF2599262.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13170..13487)
                     /locus_tag="PC460_000354"
     CDS             complement(13170..13487)
                     /locus_tag="PC460_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF2599263.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13502..13813)
                     /gene="rplU"
                     /locus_tag="PC460_000355"
     CDS             complement(13502..13813)
                     /gene="rplU"
                     /locus_tag="PC460_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF2599264.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13914..15335)
                     /locus_tag="PC460_000356"
     CDS             complement(13914..15335)
                     /locus_tag="PC460_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF2599265.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15353..16054)
                     /locus_tag="PC460_000357"
     CDS             complement(15353..16054)
                     /locus_tag="PC460_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF2599266.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16056..17900)
                     /locus_tag="PC460_000358"
     CDS             complement(16056..17900)
                     /locus_tag="PC460_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF2599267.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18219..19412
                     /locus_tag="PC460_000359"
     CDS             18219..19412
                     /locus_tag="PC460_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2599268.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19624..20706)
                     /locus_tag="PC460_000360"
     CDS             complement(19624..20706)
                     /locus_tag="PC460_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2599269.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(21270..22061)
                     /locus_tag="PC460_000361"
     CDS             complement(21270..22061)
                     /locus_tag="PC460_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599270.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(22065..22394)
                     /locus_tag="PC460_000362"
     CDS             complement(22065..22394)
                     /locus_tag="PC460_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599271.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(22879..23292)
                     /locus_tag="PC460_000363"
     CDS             complement(22879..23292)
                     /locus_tag="PC460_000363"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF2599272.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(23333..24463)
                     /gene="rodA"
                     /locus_tag="PC460_000364"
     CDS             complement(23333..24463)
                     /gene="rodA"
                     /locus_tag="PC460_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF2599273.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(24548..24832)
                     /gene="minE"
                     /locus_tag="PC460_000365"
     CDS             complement(24548..24832)
                     /gene="minE"
                     /locus_tag="PC460_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF2599274.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(24850..25647)
                     /gene="minD"
                     /locus_tag="PC460_000366"
     CDS             complement(24850..25647)
                     /gene="minD"
                     /locus_tag="PC460_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF2599275.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     VNEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(25671..26354)
                     /locus_tag="PC460_000367"
     CDS             complement(25671..26354)
                     /locus_tag="PC460_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF2599276.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(26452..29430)
                     /locus_tag="PC460_000368"
     CDS             complement(26452..29430)
                     /locus_tag="PC460_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF2599277.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(29442..29930)
                     /gene="mreD"
                     /locus_tag="PC460_000369"
     CDS             complement(29442..29930)
                     /gene="mreD"
                     /locus_tag="PC460_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF2599278.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(29945..30838)
                     /gene="mreC"
                     /locus_tag="PC460_000370"
     CDS             complement(29945..30838)
                     /gene="mreC"
                     /locus_tag="PC460_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF2599279.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(30848..31858)
                     /locus_tag="PC460_000371"
     CDS             complement(30848..31858)
                     /locus_tag="PC460_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2599280.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(31979..32638)
                     /gene="radC"
                     /locus_tag="PC460_000372"
     CDS             complement(31979..32638)
                     /gene="radC"
                     /locus_tag="PC460_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF2599281.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(32683..33267)
                     /gene="maf"
                     /locus_tag="PC460_000373"
     CDS             complement(32683..33267)
                     /gene="maf"
                     /locus_tag="PC460_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF2599282.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(33487..34458)
                     /locus_tag="PC460_000374"
     CDS             complement(33487..34458)
                     /locus_tag="PC460_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF2599283.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(34474..35049)
                     /gene="rsxA"
                     /locus_tag="PC460_000375"
     CDS             complement(34474..35049)
                     /gene="rsxA"
                     /locus_tag="PC460_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF2599284.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(35064..35657)
                     /locus_tag="PC460_000376"
     CDS             complement(35064..35657)
                     /locus_tag="PC460_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF2599285.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(35670..36239)
                     /locus_tag="PC460_000377"
     CDS             complement(35670..36239)
                     /locus_tag="PC460_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF2599286.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(36239..37216)
                     /locus_tag="PC460_000378"
     CDS             complement(36239..37216)
                     /locus_tag="PC460_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF2599287.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADISSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(37239..38576)
                     /gene="rsxC"
                     /locus_tag="PC460_000379"
     CDS             complement(37239..38576)
                     /gene="rsxC"
                     /locus_tag="PC460_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF2599288.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(38792..39736)
                     /locus_tag="PC460_000380"
     CDS             complement(38792..39736)
                     /locus_tag="PC460_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599289.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(39847..41121)
                     /locus_tag="PC460_000381"
     CDS             complement(39847..41121)
                     /locus_tag="PC460_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2599290.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(41253..41624)
                     /locus_tag="PC460_000382"
     CDS             complement(41253..41624)
                     /locus_tag="PC460_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2599291.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(41852..42451)
                     /locus_tag="PC460_000383"
     CDS             complement(41852..42451)
                     /locus_tag="PC460_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599292.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(42652..42864)
                     /locus_tag="PC460_000384"
     CDS             complement(42652..42864)
                     /locus_tag="PC460_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF2599293.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(42937..44652)
                     /locus_tag="PC460_000385"
     CDS             complement(42937..44652)
                     /locus_tag="PC460_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2599294.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNITSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(44652..45206)
                     /locus_tag="PC460_000386"
     CDS             complement(44652..45206)
                     /locus_tag="PC460_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2599295.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(45199..45801)
                     /locus_tag="PC460_000387"
     CDS             complement(45199..45801)
                     /locus_tag="PC460_000387"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF2599296.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(45810..48458)
                     /gene="polA"
                     /locus_tag="PC460_000388"
     CDS             complement(45810..48458)
                     /gene="polA"
                     /locus_tag="PC460_000388"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF2599297.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            48610..48697
                     /locus_tag="PC460_000389"
     tRNA            48610..48697
                     /locus_tag="PC460_000389"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48644..48646,aa:Leu,seq:caa)
     gene            complement(48884..49327)
                     /locus_tag="PC460_000390"
     CDS             complement(48884..49327)
                     /locus_tag="PC460_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599298.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(49330..49527)
                     /locus_tag="PC460_000391"
     CDS             complement(49330..49527)
                     /locus_tag="PC460_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599299.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(49719..50585)
                     /locus_tag="PC460_000392"
     CDS             complement(49719..50585)
                     /locus_tag="PC460_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2599300.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(50811..51680)
                     /locus_tag="PC460_000393"
     CDS             complement(50811..51680)
                     /locus_tag="PC460_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2599301.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(52186..52407)
                     /locus_tag="PC460_000394"
     CDS             complement(52186..52407)
                     /locus_tag="PC460_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599302.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(52909..53889)
                     /locus_tag="PC460_000395"
     CDS             complement(52909..53889)
                     /locus_tag="PC460_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599303.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(54053..54925)
                     /locus_tag="PC460_000396"
     CDS             complement(54053..54925)
                     /locus_tag="PC460_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2599304.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(55089..55640)
                     /locus_tag="PC460_000397"
     CDS             complement(55089..55640)
                     /locus_tag="PC460_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2599305.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(55660..56562)
                     /locus_tag="PC460_000398"
     CDS             complement(55660..56562)
                     /locus_tag="PC460_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2599306.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDD
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(56577..58949)
                     /locus_tag="PC460_000399"
     CDS             complement(56577..58949)
                     /locus_tag="PC460_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF2599307.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(59170..59859)
                     /locus_tag="PC460_000400"
     CDS             complement(59170..59859)
                     /locus_tag="PC460_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599308.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVYDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(60100..61479)
                     /locus_tag="PC460_000401"
     CDS             complement(60100..61479)
                     /locus_tag="PC460_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2599309.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENVNKYIRIKGTMTRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(61473..62186)
                     /gene="rgbR"
                     /locus_tag="PC460_000402"
     CDS             complement(61473..62186)
                     /gene="rgbR"
                     /locus_tag="PC460_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF2599310.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(62436..62996)
                     /locus_tag="PC460_000403"
     CDS             complement(62436..62996)
                     /locus_tag="PC460_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF2599311.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(63092..63895)
                     /gene="zupT"
                     /locus_tag="PC460_000404"
     CDS             complement(63092..63895)
                     /gene="zupT"
                     /locus_tag="PC460_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF2599312.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(64046..65209)
                     /locus_tag="PC460_000405"
     CDS             complement(64046..65209)
                     /locus_tag="PC460_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2599313.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(65269..66456)
                     /locus_tag="PC460_000406"
     CDS             complement(65269..66456)
                     /locus_tag="PC460_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599314.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(66819..68519)
                     /locus_tag="PC460_000407"
     CDS             complement(66819..68519)
                     /locus_tag="PC460_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599315.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(68774..69049)
                     /locus_tag="PC460_000408"
     CDS             complement(68774..69049)
                     /locus_tag="PC460_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2599316.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(69168..69740)
                     /gene="mobB"
                     /locus_tag="PC460_000409"
     CDS             complement(69168..69740)
                     /gene="mobB"
                     /locus_tag="PC460_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF2599317.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            complement(69724..70905)
                     /locus_tag="PC460_000410"
     CDS             complement(69724..70905)
                     /locus_tag="PC460_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF2599318.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKFSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(71116..71850)
                     /locus_tag="PC460_000411"
     CDS             complement(71116..71850)
                     /locus_tag="PC460_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599319.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            72212..73117
                     /locus_tag="PC460_000412"
     CDS             72212..73117
                     /locus_tag="PC460_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2599320.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(73277..74119)
                     /locus_tag="PC460_000413"
     CDS             complement(73277..74119)
                     /locus_tag="PC460_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2599321.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(74103..74861)
                     /locus_tag="PC460_000414"
     CDS             complement(74103..74861)
                     /locus_tag="PC460_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2599322.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(74878..75348)
                     /locus_tag="PC460_000415"
     CDS             complement(74878..75348)
                     /locus_tag="PC460_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2599323.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(75348..76091)
                     /locus_tag="PC460_000416"
     CDS             complement(75348..76091)
                     /locus_tag="PC460_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF2599324.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(76095..76508)
                     /locus_tag="PC460_000417"
     CDS             complement(76095..76508)
                     /locus_tag="PC460_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2599325.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(76534..77286)
                     /locus_tag="PC460_000418"
     CDS             complement(76534..77286)
                     /locus_tag="PC460_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2599326.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(77526..78920)
                     /locus_tag="PC460_000419"
     CDS             complement(77526..78920)
                     /locus_tag="PC460_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2599327.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(78940..79149)
                     /locus_tag="PC460_000420"
     CDS             complement(78940..79149)
                     /locus_tag="PC460_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF2599328.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(79178..80623)
                     /locus_tag="PC460_000421"
     CDS             complement(79178..80623)
                     /locus_tag="PC460_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2599329.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            80733..81527
                     /locus_tag="PC460_000422"
     CDS             80733..81527
                     /locus_tag="PC460_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2599330.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            81578..82900
                     /locus_tag="PC460_000423"
     CDS             81578..82900
                     /locus_tag="PC460_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2599331.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(83000..84217)
                     /gene="cdeC"
                     /locus_tag="PC460_000424"
     CDS             complement(83000..84217)
                     /gene="cdeC"
                     /locus_tag="PC460_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF2599332.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(84400..84580)
                     /gene="ssrS"
                     /locus_tag="PC460_000425"
     ncRNA           complement(84400..84580)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC460_000425"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(84695..85600)
                     /locus_tag="PC460_000426"
     CDS             complement(84695..85600)
                     /locus_tag="PC460_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599333.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            85812..86210
                     /locus_tag="PC460_000427"
     CDS             85812..86210
                     /locus_tag="PC460_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599334.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(86333..87349)
                     /gene="ccpA"
                     /locus_tag="PC460_000428"
     CDS             complement(86333..87349)
                     /gene="ccpA"
                     /locus_tag="PC460_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF2599335.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(87602..87802)
                     /locus_tag="PC460_000429"
     CDS             complement(87602..87802)
                     /locus_tag="PC460_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599336.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(87836..88015)
                     /locus_tag="PC460_000430"
     CDS             complement(87836..88015)
                     /locus_tag="PC460_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599337.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(88197..88403)
                     /locus_tag="PC460_000431"
     CDS             complement(88197..88403)
                     /locus_tag="PC460_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599338.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(88451..88876)
                     /locus_tag="PC460_000432"
     CDS             complement(88451..88876)
                     /locus_tag="PC460_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599339.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            89048..89275
                     /gene="acpP"
                     /locus_tag="PC460_000433"
     CDS             89048..89275
                     /gene="acpP"
                     /locus_tag="PC460_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2599340.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(89419..90423)
                     /locus_tag="PC460_000434"
     CDS             complement(89419..90423)
                     /locus_tag="PC460_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2599341.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(90437..91339)
                     /locus_tag="PC460_000435"
     CDS             complement(90437..91339)
                     /locus_tag="PC460_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2599342.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEDLINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(91535..92710)
                     /locus_tag="PC460_000436"
     CDS             complement(91535..92710)
                     /locus_tag="PC460_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2599343.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(92797..93642)
                     /locus_tag="PC460_000437"
     CDS             complement(92797..93642)
                     /locus_tag="PC460_000437"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF2599344.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(93740..94537)
                     /locus_tag="PC460_000438"
     CDS             complement(93740..94537)
                     /locus_tag="PC460_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2599345.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(94556..95566)
                     /locus_tag="PC460_000439"
     CDS             complement(94556..95566)
                     /locus_tag="PC460_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2599346.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(95585..96367)
                     /locus_tag="PC460_000440"
     CDS             complement(95585..96367)
                     /locus_tag="PC460_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2599347.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(96426..97562)
                     /locus_tag="PC460_000441"
     CDS             complement(96426..97562)
                     /locus_tag="PC460_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF2599348.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(98271..98738)
                     /locus_tag="PC460_000442"
     CDS             complement(98271..98738)
                     /locus_tag="PC460_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF2599349.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(98817..99359)
                     /locus_tag="PC460_000443"
     CDS             complement(98817..99359)
                     /locus_tag="PC460_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599350.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(99411..100097)
                     /locus_tag="PC460_000444"
     CDS             complement(99411..100097)
                     /locus_tag="PC460_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2599351.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(100097..100957)
                     /locus_tag="PC460_000445"
     CDS             complement(100097..100957)
                     /locus_tag="PC460_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599352.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            101092..101892
                     /locus_tag="PC460_000446"
     CDS             101092..101892
                     /locus_tag="PC460_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF2599353.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            101917..102486
                     /gene="yfcE"
                     /locus_tag="PC460_000447"
     CDS             101917..102486
                     /gene="yfcE"
                     /locus_tag="PC460_000447"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF2599354.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT        32669 a        18658 c        11000 g        40325 t
ORIGIN      
        1 ataaaataat taaagataaa aggagtattt tatgaacata cttggattaa tagttgaata
       61 taacccattt cataatggac acatatatca tttatctaaa tctatagaaa aaacaaatgc
      121 aacacatact gtagcaataa tgagtggaaa ttttctacaa agaggcgaac cagctttatt
      181 tgataaatac actagagcaa aaattgctgt cgaaaatggt gtagacttag ttatagaatt
      241 accaacttta tttgcttgtc aaagtgctga aattttttct catggtgcta taacaatttt
      301 aaattcacta aactgcataa attctatatg ttttggtagt gaagaaggaa atacagaaat
      361 tctttataat atagctgaaa ttttagcaaa tgaaccaaat gaatttaagt tacttttaaa
      421 aaaatattta gatgatggtc tgttatttcc aacagctaga gctaatgcac tttttgatta
      481 tatacatcaa gacaaaaaaa ataattttat acaaaattat tcaaaggaac aattatcaaa
      541 aacattaaat tcatcaaaca acatactggg tatagaatac ataaaaaacc tcatccattt
      601 aaacagtagt ataaaaccat ttactatctc cagggtccaa tcagagtata attccgaaga
      661 aattgagagt gatatttgtt ctgctactgc tataagaact tcacttaaag aaaatacaga
      721 cctttttcat ttaaaaaatg ttattccaga aaaaacttat aacatcctaa atgaaaaaat
      781 agataatggt tttttaccta tgtttgatga cttcttcttt gatattttaa aaacaattat
      841 acttagagat atggacactt taaaagagta ttttgaagta aatgaaggca tagaaaacag
      901 aatctataaa gatatcttta catctgaatt tctacatgaa ctacaattag caattaaatc
      961 caagcgatac actttaacca aaatcaaacg aactctaaac aatatattgt taggtattaa
     1021 aaaaagcgac atgcaactta taaaaaacat agatactata ccttatataa gaatacttgc
     1081 ttttaatgat aaaggtagag aaattctaaa agagataaaa aataaatctg aaataaaaat
     1141 aataaacaag ttttcaaata tatcattttc attagatgat acgattttta aaacactcat
     1201 aagttatgat ataaaagcta caaatatata taattcaata tattataaaa acaataaaga
     1261 tttagtaaaa ggccctatgg atttttatac tcatccaata tatgtaaagt agttttttaa
     1321 aataactata tttataaaaa tcattttatt aactctttat tatataagat acaatagtct
     1381 tactttattt atgtacacat gcttttagga tgacaacatc aacttaaata ttatgttact
     1441 ttctaaattt aagtaaatat tgccatccta aaaattttac aatttagctt cagatttaaa
     1501 agtaaagttt aaaatattat atttaaatct aatttaatat tttttgtttt gtaaatatta
     1561 ttaaattaat atttctaata tattttccct ggatttaaaa tatttttagg gtcaaaagct
     1621 aactttatat ttttaattaa catcatataa gcatctgaat ttgattcatg taagtactct
     1681 tttttagcat atcctatgcc atgttcacca gaaacttgac cactaagttc tctagatttt
     1741 ttatacatat agtcaaaagt ttcttttaat cttattttcc atgtattatc atccatacca
     1801 tcttttaaaa tatatatgtg tagatttcca tctcctgcat gaccaaaact ttttattctt
     1861 atttttaatt tgtcttgaag ctcgtgagtg tatcttataa actctgctat cttatctctt
     1921 ggaacaacga catcacattc atccatttgt gtcgtagatg cctttatagc ttctaaaaat
     1981 gcacctctgg ctgaccatat agaatcgttt ctttcttgtg tatctgatat aaatacatct
     2041 aatgcaccat tttctaaaca taaattagct actttttcat attctttttc tatatcttca
     2101 gtgctatttc catcaaatgt aagcaaaaga taagcatctg atgtattatc tggaaacttt
     2161 ttacctaaaa attcttctgc tgccaatatc acatctcttt ccataaattc tatagccgtt
     2221 ggtattgatt ttgattttat tattttagga acagtttcta ttgccattga taaatctgga
     2281 aatggtataa gcaaacttat acttttcttt ggtaaaggaa gcaattttaa tatagcttta
     2341 gtaactattc ctaaagtacc ttctgagccc actaataaat cctttatact atatccagaa
     2401 ctatttttaa ccacctttcc acctacattt ataatttctc catttggtag tacaacttca
     2461 agacctctta cataatccct tgtaacgcca tattttactg ctctcatacc acctgcatta
     2521 gtgtttatat tacctgcaat tgtagcactt ttttcacctg ggtcaggtgg gtaaaataaa
     2581 tctctatctt gaacatattg acctattgtc atcaataaaa cacctggttc tactgtcaat
     2641 gttaaatttt cataatcaac ttcaagtatt ttattcattt tacatagatt aatcattatt
     2701 ccaccattta tggcaacagc agcacctaca agaccagtac cttgacctct tggtgtgact
     2761 ggtatattat ttttatatgc gtatttcatt attttagata cttgttcagt ttcaagtaca
     2821 tttactaaaa cttcagggta cttctcaatc cctccaagtt catcatgtga aaaatcttca
     2881 tttatatcac ttccaactaa tacattttct tctccacaag tatctattaa aaaatctata
     2941 tcttttttat cgattaattt atacatttct atccccctat ttagctgctg atgccatata
     3001 tttttgatta ttatctttgt tattttctat tttttcaatc aatttaggaa taatatcata
     3061 aatatcacct attaaagcta aatgtgctac atcaagtata ggcgctttat catctttatt
     3121 tattgctact atatagtcag caccctgcat ccctgcaaca aattgaacag ctccagatac
     3181 accacaagtt attatcaatt taggttttac agttctacca ctaagaccta tttgtttttt
     3241 agcatctatc caacctgctt ctattacagg tcttgttcct gccacttgag cgtttaactt
     3301 atcagctaat ttatatatca tctccatatc ttcttgtttt tttattgctc tacttgccac
     3361 aactatcact tcagcttctt ccagaccaac ttctttattt ttttctttta cactcagtac
     3421 cttaatttta gattttagtt cttttttttc tactttacat aaagttatct ttcctgtagt
     3481 attctctatt ttttctggtg ctgaaaatat tttatatctt acagtggcaa attgtggtct
     3541 attattaggt gtgtttatat gagccattat atttccacca aatgctggtc ttatttggtc
     3601 taaatcagta tttgattgaa tatcaagtat tgtacaatct gctgtaagac ctgttctaaa
     3661 tctagcggca agtcttggag ctaatgacct acctaatgta gttccaccaa ctaatactat
     3721 cgttggtttt attttatcta taaagttttc tattgctttt gaatatggtt ctatcctaaa
     3781 atctttaaat tcatcttcat catatacaaa tacttcatcc accccataag aaatcaaatt
     3841 actgcactta tcttttatgt cttttcctac aaatacacaa tacacaggtt gttttatttt
     3901 tgatgccatc tcacgagctt tacctataag ctcgtacgtt acaggatgta tatttccatt
     3961 atgatgttca acatatactg caattcccct ccattcatct ttattaattt gtactttact
     4021 actctctata aattcaaatg cgccttttgg tccacttttg acacacaact tgcacatctt
     4081 acagccagca cttatttcta ctatacctga cttttcttct atagccccaa aaggacatat
     4141 ttctaaaatt ctctttaagt cagttatttt atcttgattt acaactatct ttgacattta
     4201 ttactccccc ttacaccaac tttaattcct ctaatttatt tacaagtgca aaacttagtt
     4261 catctgcatt tcctcttaca atatttttat ctgtatttgg ctttggagga aatattctta
     4321 caacttgagt aggagaacca ttaagtccat agttattttc atttttatct tcaaagtcat
     4381 ctaagctata tacaggtatt tctctatctt tagtattaag ttttaattta aatgaaggta
     4441 gtcttggttg aaaaatgtct ttttctacag ttattaaaca tggatattca atctcacata
     4501 cttgtaaatc atttggcata tctatttcaa caactatact ttcatcttta acttcaatta
     4561 attttgtaac gttagtaaca tgaggtatat ctaaaaattc agctacttca ggtcccacct
     4621 gtgctgtatc accatcagta gtttgttttc cacaaattat taggtctacc tttcccatct
     4681 tcttaatacc ttgtgatatg gtataggaag tagccaatac atctgctcct ccaaatctcc
     4741 tatcacttat taatgctcct tcatcagctc ccatcataaa actttcttca attaccttct
     4801 ttgcctgagg aggacccatg ctaagtactt ttagattcgc tttcttatct tcctttattc
     4861 taagagctgt ttctaaagca tataggtcat agggattcat ttttgaatct actccatctc
     4921 tttttagtac tcctgtagtt tcatcaactt ctactgaagt cgttccagga acttgtttaa
     4981 tacatgtcaa aatttccata aagtacactc cttttttatt taactataac tgatactcca
     5041 tcaggtataa ctactacttt gctctcctta ccaactatac tattcgccat ttctaaagct
     5101 tctttaaagt taaatgcatg tttcatatgc atttttttta tcatttcagg attacactta
     5161 tctgttacca ttatcacagt atatttagat aatattctcg ctagtatctg aaattcccat
     5221 tggtctggaa tcgtatctac tctatcgaca cttctaattt tatctaaaag ttgttttgga
     5281 gaatctgcat ttgctacatt ttcataaaaa gattgacctc catgaccatc attacatgct
     5341 gaaatcatta ttattactcc accgtcttta caagtagctt cagctgctgt cattccttta
     5401 acagattgat aaatattttg gtctagtgga tatccaccat ttgtagttac aacaatatca
     5461 gattttatag aatttacttt tgataactct gttacaaatc tacatccttt agtatgtgct
     5521 ttttcgacat caccagcaaa ggaagcaatt atttttttat ctttatctat tacaacattc
     5581 aatatgaatg ctaattttgc aactttagct gcatatacca tatcctcatg aataggatta
     5641 ttattcaatt ttccagttct tgaattatca ctatctataa agtcactaca gtgattgtac
     5701 attatagtct ttgcagatgc gattcctgga agtacgcttt ttctgccacc tgaaaaacct
     5761 gcaaagaaat gaggttctat aaaaccttct gctatcaaaa gttctgcctc atatgctagt
     5821 ttgtttatat ataaatctcc acctgaaggc aatatcccta ctttacacat agatttatca
     5881 tcagtagata tatgcataac tatatcttca ttatctacta tttcttctcc cattttataa
     5941 attagctctt cccttgtact tggcctatga aatccagttg ccactataat cttcacatca
     6001 atttctggat taacattcct aatccttctt aatataattg gcatagttat tctacttggt
     6061 acaggtcttg tatggtcact tgtaattatg actacattct ttttcccctt aaccagttct
     6121 tccagagaat tagagcctat aggattattc atagattctt caactatctc ttcttgagtc
     6181 ttatgttcta tataattatc cgattttgac tccaaaacac cgagaaaacg ttcatcaggt
     6241 atttctaaaa tcattccttg ttttgaatac ggtactttta actttaccat ttttaatctc
     6301 ccattataat aaactataaa attggtatgt ggtcatacca gttttacatt tatataatag
     6361 taccattatc taccatttgc aagaataatt tattttataa caaactttta attttcattt
     6421 tttctataat ttttacttat aaaatccata tgttcctttg ctttctcaca tgcaagttct
     6481 acatcatttt ctacaactgc attgtatata gcttcatgct gtttaagcaa tatattttca
     6541 ttaaaaggtt ctgcgattat tttttctctt gcatcttcta taaatccatc aaataatttt
     6601 gatgctgtca taaaaagact ttgaattaaa taatttttac ttaatgttgc tagtttactg
     6661 tgaaattttt ggtctaataa taccagtact cgattattat cttttttatt agtttctacc
     6721 ctcatttcat ctattatatg tttcaattct ataacttcag aagaagttat tctctttgaa
     6781 gcaagttttg caatctcaat ttctaggatt attctaagct ctaatatatc ttcaagtgta
     6841 ccattattaa gtttaaaaat catagataaa ggttctatta aagttttttc tatatttgta
     6901 catataaaat ttccttcacc ctgtctgctt tctatcactc ccatagtctc taatacccta
     6961 atagcttccc ttatggaagt tctactcaca ttcatttgtt cagacagttc tctttctgat
     7021 agtaatttat cccccttttt taactctcca tttagtattt tatattgtat ttgctcaatt
     7081 acttgttcat aaactttttt actagctata ctattaaaca ttttttcctc caatatgata
     7141 atcgttgttg taatatatat ctattatata tcttaagcca ttacattcat atatttttat
     7201 tacttttaat ataatatctt ataactcaaa tttacaggtg atattatgaa aaaaaataaa
     7261 attattgctt tttttgctcc atctatcata gttggagttc tagttttagg gattatccta
     7321 tacccaaatg aagctataaa atctgcaaga gaaggctttt ctatatggtc taatgtttta
     7381 gttccatcac tactaccatt tataattgga gcaaacttaa ttgttgattt aaaaatagtg
     7441 gatattatag ggtttatcat caatcccata actagatttg tatttaatgt ttcaggaaag
     7501 agcgctttag cttttgctat atctacagta tctggttatc cagtaggagt aagtttagct
     7561 tctgaattta ggagtaatgg acaaatttct aagtatgaag cacaaagact tgtgtctttt
     7621 tgttctacat caggaccttt attcataata ggttcggttg gtacaggtat gttccaaaat
     7681 tcaaacttag gttatttaat gattctctgc cattatctag gaactatatt ggttggtctt
     7741 ttctttagaa attatggtaa tgaaaacctt ccaaagacaa aattggattt aaaaacaaat
     7801 ataaaaaatg taatcaacat tagaaattca agtgggaatg gtttttttgt tttatttgga
     7861 aatgctgtat ttaatggagt aaacacatta cttacagttg gtggatttgt aattgttttt
     7921 tcagtagtat ttaaaatact atctcttttt aatgtaatct cattaatagc atcagttata
     7981 tatttgcctc tttcattttt aggtgttagt aaagaactct gtcaagcatt tgtaagtggt
     8041 ctatttgaaa ttactatagg atgcaataaa gtatctagta taacaagttc tccagaaatt
     8101 ttaagagcat ctttagctag ttttttgata ggatttagtg gattatccat cctagctcaa
     8161 tgttgtacat ttctatcaaa aacggacata gatttaaaat tatacatact aagtaaattt
     8221 gcgcatggag ttttagctgc tatctttata tttatacttt atccaattgc aaactctgct
     8281 gtgttggttt ctagctttgc tgatacatac aacgttatat ataataattt aatctggttt
     8341 tattacctta gctattatga tacaattctt caaattgtag taataattta tctaatctca
     8401 gccgtattta ttgcaaaaaa acatatgaaa actttatcta caaatgggaa actaatgcga
     8461 tataaaaata cttttttcac taagaaattt tcacgaaaaa aataaaacta aaaataaaac
     8521 taataaaact taactaaata cttccatttt tttattatat ttgtcataat tttaataacc
     8581 tttttaatta gtttaacagc atagttattt catcacaatt taataatttt attagaaata
     8641 tttttcaaaa aattctcctt aatcacatat atcacacttg tataagttta ctgggaaatt
     8701 tttccattat ataatatttc atttatatta tataataaat accgacctaa acttaccaaa
     8761 tcaaactaag aggagtggtt gaaaatggca acaagaccta tagaaataga cgaatataaa
     8821 aagataatgg aactattaca tactggcttt acttatagtg aaaatggcgt tgaaaaaaga
     8881 tttagaaaaa atcctaaagt tgcattggca ctaatgttag aagctaattt aggtctaaga
     8941 atatcagata ttttaagatt aaagataggt aattttaaag gtaatatgtt ggaaataaat
     9001 gaaaaaaaga cgggcaaatt acagtataga ccagttaata aaaatataat tgagtccata
     9061 aatgaacatg caagaaagta taatcttaaa tcaaatgact acttagtaaa tataaagaca
     9121 aaggctattc aaaaacagtt gaggataatt tgtaaacact taaatctgta taatataagt
     9181 acacatagct ttagaaagct ctatgctact actcaatttg aaaagagtaa caataattta
     9241 gagttggtta aagaactttt aaatcacagc tctgtagcca ctacacagcg atatataagg
     9301 gtaactcagc aagctattaa tgaagctagt gaaaatttct ttattggtta attataacgt
     9361 atacattttt ctattatctt attaattcac cagatattaa aaaagagtgt ctcaaaatga
     9421 actttttagt tcataagaca ctcttttttt ataaaatcaa atatattttt attatttcaa
     9481 cactggaaaa aaggcttatc tctctatttt gagacaccct attaaatttt cataattctt
     9541 gcataaacat attaatctat cttcaataat tttttagctg attcactgta attatttctt
     9601 tttacaattt ccaaaactct atcaatctca tgttctgact ttgcacttac aaaaatcgcc
     9661 aattcttcat catctataat atcataacta tctacatatt tatctaaaca ttctaaaatt
     9721 ccatctttat tattataccc accaaatcta actactgcac tacactcatc ttctataagt
     9781 ccacttaaat ccaatttccc ttttgaaaaa gactttatat ttgaacttct attatctatt
     9841 atatctataa tatctgcata aacagcactt cctgttgcac ctcttccagc tcccttacct
     9901 acaaaagaaa gttcacctac tgaatcacct actaaaataa ctacgttgaa ctcattatct
     9961 atctttgaaa gcatattatt attgtccact aatgctggtc taacaaatcc acttacttct
    10021 cctgattcat acttacttct agcaaccaat tttatcttac aatttaattt ttttgcatat
    10081 tctatatctt tttcatctat tgaagttatt ccttctaata ataaatcttt ccaatatact
    10141 atttttccat aagataaagt agacaatata gataactttc tagcactatc atatcctaaa
    10201 acatctgcat ctggattaac ttctgcaaat cccaactctt gtgcctgttt taaagcgaca
    10261 tcatacggta aattttcatc atacatcttt gataatataa aattacaagt cccatttaaa
    10321 atagcgttta tgctctttat attgttgcct tccagtgatt ctatcaatgg ttttaaaact
    10381 ggaataccac ctgccacaga tgcttcaaac ttgatgctaa cattatttaa gttagctaat
    10441 tctataaggt cactaccttt ttcagccagt aaatctttat tagctgtcac tacatgtatt
    10501 tttttattta acgctcttgt tacataatca aaagctggat ttaatcctcc aattacttca
    10561 actagtatat caatatcctt gtcaaatata tcttctatat cagtagttat cttatcaaag
    10621 tacttcttat acttgtgttt ttctttattt ttaactaaaa ttccaacaac ttctatatct
    10681 ctttttgaag tattattttc tattaaatca attaatccac ttcctactgt tccataccct
    10741 aatattgcta tcttcactgt aacatacccc catcattttg attatattaa attatttata
    10801 tatataaata taccaataaa aaaagcttga gtataaactc aagccatatg acgggtttat
    10861 actcatcttt cagcataaat gctgcaggat ttagcaccaa tgtataatta aatatactgg
    10921 ttgccgggct tcacagggcc agtccctctg ccactcttga taagattgct aatttaaatt
    10981 tatgtttaat agtataatac aatttcttcc aattgtcact atttttttaa cttatttccc
    11041 taaaaggata taaactatta tctaacagct cttttagttg gtttaccctt ttttgtaaca
    11101 ttattatttt tagtctttgc tttcgcctgt tcatgtcctg gaaaaagtat tttaggctct
    11161 tcaatattcc tcttgtaaat agtaaattta aaaccaatag cctgcacaaa ttcagctctt
    11221 aaagcttcac atactgcatt agcagtttct ttagtatcaa gacctgcatt ttctaatata
    11281 gtaactttta ctaattctct agttcttaac ataccatcta attgctctaa gaaactctct
    11341 gtaacacctt ctttaccaat ttgtgtagta ggctttaaag tatttgccaa agatcttaaa
    11401 tacgcccttt gttttccttt taacatattc tcacctctat aattctatac ttaaattatc
    11461 tataatctat atttaaatta attgtaaaac tcaaattcta attcataaat tttaaccaca
    11521 tctccatctt ctattcccat ttctctaagc ctatcaaaaa caccttgact ctccatagct
    11581 ttttggaaat attgaaggga ttccatatca tcaaaattta cagaatacat tattcttcta
    11641 agtgctttac ctgtaactac atacacacca tcttcaatat ctatacttaa ctcttcttca
    11701 gtaccaatat caagttctgg tctatacatt tcttcttctg aaactagctc cacatcttct
    11761 acttctttta gcattttaga aacataggct attacttcat ctatgccttg acgagttgct
    11821 gctgacattt taaatactgt atatcctctg ccttctaatt cactcttaaa cttctcaaac
    11881 ttactctcat cttctaatat atcaaattta tttgcaacta caacttgtgg tctctttgaa
    11941 agcttttcat tgtacaattt aagctcatca tttatcttgt caaaatcttc aatagggtct
    12001 cttccctcta atccagatat atctacaata tgtatcaata cctttgttct ctcaacatgt
    12061 cttaagaact catgacctaa tcctatgcct tcagaagcac cctctataat tccaggtata
    12121 tctgcaagta caaaactatc tccaaactta gtttgaacaa cacctagatt tggtgtaagt
    12181 gttgtaaaat gatagtttgc aattttgggt ttagctttag ttacaactga tagaaatgtt
    12241 gatttaccta catttggaaa tcctaaaaga ccaacatctg ctatcatttt aagttcaaga
    12301 gttatccatc tttcttctcc atctgtacca gattttgcaa aagctggagc ttgtcttact
    12361 gcatttgcaa agtgttggtt acctttacca cctataccac cttttgcaac tatagctgta
    12421 tctccttctt tttttaaatc tgctaataca agacctgtag cctcatctct tattactgta
    12481 cctgctggta cttttaatac taaattttct ccatttttac cagctctttt tcctttgctt
    12541 ccgtcgccac cattttgagc ttgatatttt ttctggtact taaagtccat tagagttcta
    12601 agccccaaat caacttcaaa tatgatgctt gctccacgac caccatctcc accgtctgga
    12661 ccaccagctg gaacgtattt ttctcttcta aatgctaccg aaccatttcc accatttcca
    12721 gccttaacaa atattcttgc cttatctata aacaaattct tcacctactt aattaaaaac
    12781 ttttaattag cattaccaaa aataccatta taaattcatc agtattataa gaaaaaggag
    12841 tgcctcaaca ctccttttat gtatattact cagctatagc tactggatat atactaactt
    12901 tttttctctt cttgtctttt ctttcgaatt taacagtacc atcaactaat gcaaataaag
    12961 tatcatcact accttttcca acattagttc ctggatgtat tttagttcct ctttgtctaa
    13021 ctattatact tccagcagtt actaattgac catcgaatct cttaacacct aatcttttag
    13081 atatagaatc tctaccattt ttagaagaac ctaccccttt tttagatgct aataattgta
    13141 aattcatgtt taacatatgg agtcaccccc tactttttta aaatttttat attttttggg
    13201 tattcccgtt taatttcttt aaccgctaat tgaaagtgat ttagtaataa ttgtgcgtct
    13261 tttacatgtg tatctttgac tatacactct atatgacctt cttctcctac tactttttca
    13321 aattcaatct tttgtaattt gtctaaagaa tttataactg caatagcatt agatgtaact
    13381 gcagcacata ctatatcata gcctatttca gcaaaatcag catgtccctt taaacaaaaa
    13441 ccttgcaaca agaaatcatc attataataa tatttaactt ttatcataac ttaaccaata
    13501 actaagcgtt tattttttct acaactatct tagtgtatga ttgacgatgt ccttgttttc
    13561 ttctgtaatc tttttttgct ttatacttga aaactataac tttcttagct ttaccttgtt
    13621 caacaacttt agcttgaaca gatgctcctt ctactactgg agaacctaat tttaattctc
    13681 catctttaga gcaagctaat acttcgttta aagtaacaac atcacctgcg ttagcttcta
    13741 atttttcaac gaataataca tcaccttcag aaactttata ttgctttcca ccagtcttta
    13801 ctatagcgta cattaaatac acctcctcgg cctcaaactc gccagtacaa ggtacttcat
    13861 ttgaagattt aaacctttac tgtgcggcat tacaattatt aattataaaa tatttacatt
    13921 ttattgtcaa ctattttatc gaaaataaca ttggttttat tgtgctttaa tgtactattt
    13981 gcgtttaaag aaatctttat attatatttt tcaccaattt tatcaattat atccatataa
    14041 tattctttta tttgttcaaa aataaaatca ttaaattcta ccattatgtt tttgtaaaca
    14101 gtatgttcac ttattctcat aatttctttt tcaatactat ctagtatata atgtacagat
    14161 tttattgttt gttcaccatc acaacatgga caagacatta aataatactt atcaatggat
    14221 tctttttctc tacgcctagc aacttctact aaaccaagtt ttgtcatacc taatacttca
    14281 gcttttcttt tatctttatt aaattcttct tctaggatgt gtaaaatctc cttttgatgc
    14341 ttctttttat gcatatcaat aaaatcgatt attattatcc cagctatgtc tctaattcta
    14401 agctgtctta caatttcttt tgcagcttca agattagttt tataaacggt ttcatcaagt
    14461 ttaccagttc caataaactt acccgtattt acatctataa ccgtaagagc ttctgttttt
    14521 tcaattatta aataacctcc actctttaac catacttttt tatttaataa tttttctatt
    14581 ttagattcta ttctgtataa atcaaataca tctctatttt cctcaagaca cagtttatca
    14641 acataagtct tatctatacc tcttaaaata gacttaagtt cagagtactt atcataacta
    14701 ttggtaatta tagattcaat atcatcattc acattatctt ttacatattt agttgcaaaa
    14761 tctaaagact tatataataa tttcggacct ataccaagtt tatattcttt taaaatgttt
    14821 tcatacttta atttcaactc ttctatatct ttctttattt catcatgagt gcaaccttga
    14881 gcctcagtcc ttattattaa tcctagattt tccttattta ctgccttagt aatttttttt
    14941 aatctaaatc gttctttttc atcagtaatt ttatttgata ttgtagttct atcattggaa
    15001 ggtatataga caatatacct tccagaaata ctaatctcag tgtttaattt tgcgccttta
    15061 gttcctattt cttctttatt tatctgaacc aaaatatcct gaccagattt tatagtactt
    15121 ccctttttta attgtaaata agcaagtttc ttaaatccaa tatctacaaa acaagcttct
    15181 attcctggta taactttttt tacgattcca cgatatatat tggatacagt cttcttattt
    15241 aaattgtctt caacaaaaag ttctgttaat ctttcatctt caagtactgc tgttttctgg
    15301 gaccctatta gggactcaat aactatcttt ttcacaactt cacctctcga gcttacatag
    15361 gagtcactaa ttccccatct tctaatacat ataaatctct tctcaatata tcaacatcta
    15421 atgggtcaat atcaagttca aacatctcta atatctttgg tataagtata ttagtattta
    15481 aattagtttt tgagcctgta gctatagtag cagtaagagt aatatgttca tcttctaaat
    15541 ttaaaacatc aaaggttctt atcataggtc ttatatctac ttcaaccatc ttaccttttt
    15601 tattcttttt tgttatgatt atttctttat tattcataaa gtcaattact tttcttttta
    15661 caaactcttt cgtcataggt cttttcaaat ctatattaaa caaatattct ccatagtcta
    15721 tcacagaaga aagagaaggt gtttgtctat caatttcttt tgctttgata aaatctatcc
    15781 catctggaag ttgaattgat actttattta aaaattcctc tactgataaa tcatcttctt
    15841 ctatttcaat atctacatac tcaccttgac tttctgtccc taaagccaaa gcattgccat
    15901 aactcatttt agggtgagga ttaaatcctt gagaatgtga taagtttatt tcagctcttc
    15961 taaacgctct ttgtaacaat ctctgaaggt caagatgtga tatatatatc atgtcgcctt
    16021 cctttttaaa ttttactctt attatcttac tcatattagc aaagccccct tcctatatca
    16081 tgtatattta ttccacaacc attgcagtta tgtctacaat cagaagtgat tttttctttt
    16141 ttagcatttt cgtcttctct aattaagaat tttttactaa tacctacatc tatatggtcc
    16201 caaggaaata cctcttcata gtttctattt ctatgtgcat aaaaatctat agaaagatta
    16261 ttcttttcca tagcttcttt ccatatatct aaattaaaat gttctgccca tccatcaaat
    16321 ttagcaccta gtctaaatgc atcatatata acttttccta ttcttctatc gcctctagct
    16381 actactgctt ccatcaaact agtctttgaa tcatgataat tgtattttat attgttattt
    16441 cttaatttat ttactaaatg tctctgctta ttaataactt cttctgtagt atcttgacca
    16501 tgccattgga aaggtgtaaa tggctttggt acaaatgtag aagtactcac tgtaacacta
    16561 aaacttctct ttagctttcc tccatttact tttctataaa tatcaataac tttataagct
    16621 aatttagcta taccatctaa atcttcataa gtctcagttg gtaaaccaat cataaaatac
    16681 aacttaacac tattccaccc catctcaaaa gctctttctg ttgcattttc caaatcttct
    16741 tctgtaacac ctttatttat aacatctcta agtctttgag ttcctgcttc tggagcaaaa
    16801 gtaagtccag attttcttac ttgttgtatt ttatcagcaa tttccattga aaaattatct
    16861 aatctaaggg atggaagcga aatcccaata ttattagatg catattcatc taccaaataa
    16921 tcagtcaact ctgataactt actataatca ctcgtactaa gtgatgataa tgatatttca
    16981 tcatatccag tattcttaac taatttatct aatatttctt taagtctttt aacactcttt
    17041 tctcttattg gtctgtatat catacctgct tgacagaatc tacatcctct agtacatcct
    17101 ctaaataatt ctagtactat tctatcatga acagtatcta tataaggtac tattagtttt
    17161 tctggataat ctacagtctc aacatctttt attatccttt tagtaggatt tttagtaatc
    17221 ccttctctat taggagttac actttgaact gtaccatctt cattgtactt gacatcataa
    17281 aaacttggta tgtatactcc ttctatgcta gatattttat ataaaaaatc ttctctagtc
    17341 gttttatttt tcttccattc tttatactca tttactactt ctaaattaac ctcttcacct
    17401 tcacctaata ttactacatc tacaaaatct gctaatggtt cggaattata agcacaagga
    17461 ccaccaacca ttataaatgg gtcttccaaa gttctttctt cttttaatat tggtatattg
    17521 gctaaatcta atatatttaa gatattagtg tatgaaagtt catattgaag tgtaaaagtg
    17581 acaaaatcaa agtttgttat aggctctctt gactctaatg caaataatgg tatacttttt
    17641 tctctcatta tattttccat atcaactgca ggagaataca ctctctcaca aaatacatct
    17701 tcatccttat ttattacatc atataatata tgactcccta agtgactcat accaacttca
    17761 tataaatctg gaaagcaatg tgcatatctt atcaattcac tattactagt atctttatga
    17821 attgaattta tttcatttcc aagatatcta gcaggctttt ctactttttt taagattttt
    17881 ttcaagtcta ctttattcat ttattaaaat tcctccaaac aagctcttta tttttattct
    17941 tatttataag taatattata cacgataaaa tattttctat caaaaatata actaaaatat
    18001 tgaatttaaa atacatatta ttcacaaaat agaatagcta aaaatattta agaatatttt
    18061 ctattaaatt tttattgaaa tacaattttt ttacaaagta tgatatatta taaacataaa
    18121 actattgttt tacatgactt atcatgacat cttttgtttc attcacctca aaatggggta
    18181 tgttacttat ataatgataa attttaggag ggttaaaaat gagtaaagtt tttgaagtaa
    18241 aaaaagatat ctactttact ggagtagtag atgaaggttt aaaagtattc gacataataa
    18301 tggaaactga gtttgggaca acttacaact cttatttaat caaggacgaa aaaacggttt
    18361 tatttgacac tgtaaaggca aattttaaag atgagttttt aagtaattta tcagaggtta
    18421 ctgatatagc taaaattgac tatgttgtaa ttcatcacac agaaccagac catgcaggta
    18481 gtttaaaata cttattagat ataaatccta atatagaagt ttattgtact aaagctgcta
    18541 aactatactt agatggtcaa ataaatagac cttttaactg tcatgtaata aaagatggtg
    18601 aaattttaaa tatcggtaaa agaaatctta gatttataac tgctccattc ttacattggg
    18661 ttgatacaat gtttacttac attgaggagg acaagactct tttaacttgt gatgcatttg
    18721 gttgtcactt tgcaagtgtt gatgcagagg tagtgaatag tgaagattat ctaaaatcag
    18781 ctaaacacta ctatgattgt atagttaagc catttgctaa acatgtttta agtgctgtag
    18841 ataaggtggt aggactaaac atagaatttg atactatatt gacttctcat ggtccaatgc
    18901 ttactaaaga cccaatggct gctgttaaaa gatatgttga gtggtctaca gaagctgtta
    18961 atacaactaa tcaaaatcaa gtttctatat tctacttatc agcttatagt aatactttag
    19021 agatggctaa aaagataaaa gaaggacttg ataaagaagg tgcaaaggct gaattatatg
    19081 atttagaaga tatgacatta acagaaatgc acgatacatt agtagtgtca aaagtaatat
    19141 tactaggttc tccaactata aacaaaacta tggttaaacc tatgtgggat ttattctctg
    19201 ttatagaccc aatggctaac caaggcaaga tagcaggagt atttggttca tttggttgga
    19261 gtggtgaagg tataactatg gctgagactt tacttaaatc tatgtcattc aaaatgcctg
    19321 tagaatctct taagaaaaaa ttcttcccta gtgaagagac attaaaagag tgtatggcat
    19381 ttggtgcaga atttgcaaaa ttagttaaat agccattaat caaacataat attatttaaa
    19441 ataaatgatt ttatatttaa aaataaatta aaatggagtt gtctcaaaat taatttttct
    19501 aatctaattt taagttcaac tcctttttta ttttctaaaa tgactgatat aatgataaat
    19561 gcaccctttg tgcgagggtg cattatttat aatatgttct atttgtacac tagtatattt
    19621 gtattaattc acatatctag cgtatgctac catccctctt ccagaattag atgccgtttc
    19681 accaatttcc actttaataa ttacattatt ttctttacac ttttttgata attctgctgc
    19741 agaaaggttc ttaccagtaa aatctaaaga aaatggctta tcatattcaa aaggatactc
    19801 atcaggtaca ccatctggag ctaatgcaac cataccatat ggtgtattca taaaccctat
    19861 tacagtaact gtatgctttg atgggtctaa atttgaattt ttgcttgtaa acttaattgt
    19921 atttattgat gagttgatta aactacacgg aactccctca tacatttcaa tttttggacg
    19981 tagattattt ccataaactt catcaagtat tgccccttgt tgatctgcta tcatagtgtg
    20041 attcccaggt ccaactggcg cagaaggctt agctggcgta ggtttcttaa cagttggcgt
    20101 tggattctgt gtctttacta cttcgccaac agctggatat gctttcttgt caaatggtaa
    20161 tgggtgagta agactcttgt atatgatgtc tgctcctgct gatttgttgg catcaatatc
    20221 tacatgatac aaacgtccat cctcaaaacg aataagattc cactgatggt catttgttgt
    20281 actatgtaca taataacatt caattcccat agcatcacat aaagctttca agctcctaca
    20341 cattccagaa caagcacctt ttttagtaac caatgtataa gcagtattgg ctccctctgc
    20401 tgcgttccag ttaacatatg ttgtgttgtt tttaatgtga ttataagcaa ccctaatctt
    20461 agcatctgtg gtcatgttct ttgtaatttt cttgttcata aagtctgctg ttgcttgtgc
    20521 taccgaatta agttgagctt gtgataactt tggaccatat atactgttgg ttccagctac
    20581 ataacttttc ggtgtctttg cagtaacatc tttagctgtc ataccataaa ctgttacact
    20641 catacatgag gttaaaacca tcattgcaat caatagttta gtagctaatt tcctgatttt
    20701 tttcatatta gcattctcct ttataatata ttttactttc aattccctac cattaattaa
    20761 gttcatttta gatttaaaat atggattatc aacatgtatg catagcaatt cacgtggtca
    20821 tagaatattt agatttcttg gaaaatctgt tctatatttc ctatatagtt cgtatacaat
    20881 ccaactcaca aacctgcatt tttgatatca tcacaataaa atgataataa ttaatatatt
    20941 caaaaatgtt ggtctgtaac aggtatgtca attttccaat tattggtaat ggcgagagtg
    21001 aggagaattg tcaataatac atttaaaatt aaaattgtat acacttcata cattctcccc
    21061 taacaacatt atacagatta taatttgtat attcaataca ttttttggaa agtataatta
    21121 tatcaaaata tgtgattatg tatagataca atccactatg gagaaatata aaagaaaaga
    21181 gtataacaaa caataaaaaa taagatgatt caaattttat tttgaaacat cttatttact
    21241 ctacaaatta agattatttg gtgaattatt taattattat tttttattaa agaaatttta
    21301 aatcttaata taaaatacgc tattatacat aataatccaa atgcaaatgc tactggaatt
    21361 aacatctccg attcaaaata aaagaaatat tctaagaaaa tatctggatt tactatattc
    21421 ccattcttgt ctaaaaaatc ttttccatta tatcctaaat gctgtataaa attacttatg
    21481 ttattataat ctgcttccct ctcatatacc atattagcat tcaatccaga agaacttaaa
    21541 ttagtgtttc catctttcat atctattaca tatagtctaa gagctttgac tttactatac
    21601 ttatctttat caagtatttt ttcaagttct aacttatatt caccaatctt tgtcatatca
    21661 tgtctttctg ccaattgctt tatttctcca tcaagttctt ttcctaaatt atacatatta
    21721 tgttgtaaac tattattgta ataccacata aatccagaaa ttaaaaatgc aattatactt
    21781 atgtaaccta atactttttt aaaccccatt acaattgatt tatgcctatc caatgataaa
    21841 gatagttctt ttattatatt acataattca tactgcattt catcaccatc atcaaatctt
    21901 tcaattgctt ttttacatgc ttcttcttca tctaatcctt ctaatttaaa ttcatttgca
    21961 gattctataa gatggcatct catttcctgt tttaattcta tagtactttt attgtcacat
    22021 tttttatata gtttatctaa atattcatct attagtttca taaattaatc cacctctccc
    22081 aaaaattgat tcataacctt tttaataaat ctccattctt gtttctttat ttctaaaaaa
    22141 tctttacctt cattagttaa attataatat tttcttcttc cacctgaact ttgttcattg
    22201 ctataataag atttaattaa atttttagat tccattcttt taagtgcaag atacattgtt
    22261 ccttctttta attcaaactc acttctttct cttacttgct ttgcaatttc atatccataa
    22321 caatctttct tttctaatat agaaacaatt aatatatcga tataaccttt taacacttct
    22381 ttatttaact gcattatgta ccaccgcctt ttcacctact actctataat gtatagtact
    22441 tatatttact ataatattac atagtactct atattgcaag gtttatattc tacaatagac
    22501 ttaaaaataa taaatagctg tatattaata tttaatctat aaatactaat atacagctat
    22561 ttatcaaatt ttaaaaataa atttttgttt tattaatgtt gtcataacaa catttacttg
    22621 gttgaaatag tattacctat aagtttaaat atatataata atagacttga ttctatttca
    22681 tattattaaa atagagtaat agtttaaatt ctactactct atctataatc tatttcctaa
    22741 ttatttaatc tcaaatttag ctcttcatca tttaaatctt tcataagatt tctacaggta
    22801 ctagatttat tccaacagaa agactagggc attaaacact tatatatctg tttctttaga
    22861 ctgtaatatt aatatttttt aaatacgttg acttttgctt tttctaactt ctctccatga
    22921 tagttctcct ctatcaagag cttttataaa tatttctgct gaagctagat ttgtagctat
    22981 tggaacatga taaacatcac aaagtcttat taacgcttgt atatctgttt catgagcctg
    23041 agatgtcaat gggtctctta aaaaaataac taaatctatt tcttgtgtta ctattaaaga
    23101 acctatttgt tggtctccac ctaaaggacc agatgctaat ctatttatat ttaattcagt
    23161 ttcatccatt attctttttc ctgtagttcc agttgcatat agtccatact tttttaaaat
    23221 tgattcataa cctatacaaa atccgaccat agtgtttttc atttggtcat gtgctactaa
    23281 tgctatattc atatatctaa tctcctcaat ttatgtttag atgtatttaa atttagaaat
    23341 ttattttctt tcttctcata cacacattta gtactaatcc taagtttgct aaagatgtca
    23401 ataacgaact tcctccataa cttacaaaag gtaatgttac tccagttact ggtattaacg
    23461 caaccgtcat acctatattt tggattatct ggtaaccaaa cattcccata acaccaacca
    23521 ctatgagggt tccataaaaa tcttttgcat ctcttgcaat tgctaaaagc cttaatagga
    23581 atatcataaa taagattatc aacactgcca ttcctattac accaagttct tcacctacaa
    23641 cagcaaatat aaaatcacta tcttgtacag gtaaaaaatc ttcttgattt tgacttccat
    23701 tgtaaagtcc tttaccagtt acgcctccag aaccaattgc aatcagagat tgcataactt
    23761 gatagtttcc ttttagagtt acatcttctg gatttagaaa agcttctatt cttacctttt
    23821 gatggtctgc catcattaaa tacatcagtg gcattgacac taatagtatg attattcctc
    23881 tttttatcaa ctttgagcta agacctgctg tgaacagcat tgcaaatatc atacaacaaa
    23941 atacaattcc tgttcccaag tctggttgtg caatcaataa tccgataaat ggaagagaat
    24001 aaactacaac aggcataact tcctttaacg tatttaattt atcctttttg gattcaagta
    24061 tctttgcata acttagtaca aatgttaatt tgactatttc agatgtctgc aagtcaagtg
    24121 gtcctaagtt aatccacgac ctagctccac cttttactgt cccaattcca ggtaataaaa
    24181 ctatggctaa caaaataaga cttattatat aaagcgcttt ataatacctg cctagaaggt
    24241 tatagtcaaa aaataatatg actataatca ttcctatccc aagtacaaaa gctaatcctt
    24301 gtttaatcaa ttgattatat gaacctgttg aattagcatg tgttgcacta ctaagtataa
    24361 caagcccaaa aataaagata gctaatactg taactataag tttccagtct aactgtttta
    24421 tcaacttaat tgtcgactta taattaatat tgattttttt caaagaatca ccgtctttct
    24481 atctttaaac atataattaa ataaaagggg catataccaa taaggatatc ccccttttaa
    24541 gcactaatta catacatcta tcttttatat tctttagtgg tatattagca actaatgcag
    24601 atacaggttt gtcatcatgc tcacctctgc atttagacat ttttatttca agaccatcat
    24661 cttctatttc tgcataatta gctattactt ttaaaatgtc tgtttttatc atctctaaaa
    24721 gttgtggtga acaatctact ctatcatgaa ctaaaactag ctttaacctc tctttagcaa
    24781 cagatttact agttttagcc tcgttagaaa aaactctaaa taaatctaac acagatgtat
    24841 cctcctttgc tacttagcca tgctaaacat ttttttaagt ctaccaaaga atccatcttc
    24901 aacttcaaga tcaagtagag gaacttcttc atttagtatc ctttttgcga tatttttgta
    24961 tgcttgacca gcaagtgact tagaatcaag tatagctggc tctcctttat ttgttgatat
    25021 aattatgctt tcatcatcag gaacaagacc taacaaatct attgctaata tttctattat
    25081 atcttgttta tccatcatgt ctccacgctt aaccatatca tttctaattc tatttataac
    25141 taatcttatc tcttttattt catttacttc taatagacct attattctat cagcatctct
    25201 tactgctgat atttctggat tagtaactac aatagctcta tctgccccag ctactgcatt
    25261 tttaaagcct tgctcaatac ctgctggaca atctattatt atatactcga aagattcttt
    25321 tagtttctca cacaggtcaa tcatctgctc aactgagaca gcatttttat ctctagtttg
    25381 tgctgctggt aataaatata gattatcaaa tcttttgtct tttattagag cttgcttaag
    25441 cctacaagtt ccttcaacta catctactat gtcataaact attcgatttt caagacccat
    25501 tactacatct aagtttctaa gtccaatatc tgcatccaca actactgttt ttttattttc
    25561 taggcttagc gcagttccta agtttgctgc ggtagtagtt tttccaaccc cacctttacc
    25621 agatgttata actataactt cgctcataat actaccccct atttttcttt ctatttgtct
    25681 aactttgaca aatagctttc gattacaatt cttccattac ttacaaaggc tatttctggg
    25741 ctaatttctg attcactttc ataattttct tcatcatctg gagcttctgc aatattatct
    25801 gctatttgta atattttagg attaagattt ccagcaacaa catatgcaaa ttcattgcct
    25861 tctgcacctg catgtacaaa accttctatt ttacccatta caaaaacatt acctcctgct
    25921 acaaccttag caccagcatt tatatctccc ataacaactg tatttgattt tgaaaggact
    25981 tttgaaccag gtttcatatc attcaatata actacatcac cttcaaattc aatattttca
    26041 ccagatctta gggtattgac atattttgtt ggaaaattac tattttcttc aattttttga
    26101 tcttcaacaa attccacatc aaatctagaa gtaattcctt ctttaagttc tagaatctcc
    26161 atgtcagtta aacaatcact attaatttta ctaattttag ccccattaaa aaagccaaca
    26221 tatgcttcca atttgtttat tattttttcc tgtatttcct caaatggagc ttctcttttt
    26281 atatttacta ttatccctct tttattaccc ttgaactcta ctagttcttg actacaaatt
    26341 tctcttaaag acataacaac ctccagagtg atatatcatt tatattcttc aaatacgctt
    26401 taaacccttt tattttttct atataaagct aaagtttata catagattga gctatctatt
    26461 aatttgtgac tcgaaattta tattttcgtc agaagaattg ttaacatcat tcgtcttgtt
    26521 gtcattttta ctattagttg gctctaatcc catataagtt ccaataactt ctctagttgg
    26581 taaaagtgag aatacacttg aatcaccttg aggaattaca catacaacag ctatttctgg
    26641 gtcatctgct ggagcaaaag caacagtcca cgtaaaagaa ccataatctt gtttaaatct
    26701 atctatttgg tcatctgtta tttttggatt taattctttt atagcttttc ttagatatga
    26761 agaattcacc ttatcagtgt cctctaatct aacttttact ccatcttcta attcttcctc
    26821 taatttcttt ctttcttcgt ctttcaaatc tttattttct aatttctttt ttatttcctt
    26881 ttccttagct aatgaaagct ctttttcttt ttctgctttc atcttatcag ctagtttaat
    26941 cgcatcattt aggttgacat tataagatga catatgactt tttaaatatt catactcgtt
    27001 atctgtaggt attttcccac ttttctcagc tgtaccagtt tttgctgcta catctattgg
    27061 aaaatcagca aaagcactct tcgctgtacc ttgtcttgca accaatttca taccttttgt
    27121 taattctttt aagttatctg gatttttaaa aggtattttt tcgactttct tctggtcatt
    27181 aattttaact gatgaataat cacttgaaac agctctatca actacagata attctactaa
    27241 attacctcca tttgctatag cagctacata tcttgatatt tgagctggtg tataagcatt
    27301 ttcaccttgt cctatagcta agttgaatgt atctgcagta ctccattttg caaaattaaa
    27361 ataactaaat actgctaaat ctgctacttc ttcaattcta tcttctttaa catccaattt
    27421 ttttagccta ttcattgttt caactctacc aggagttttc ttttctgctg cccagcttac
    27481 aatctcattg attcttttct catattcttt aggatttttt tctctagtta tatcaacaaa
    27541 atcatttgcc atttctcttt ctaaatttga ctttagaaga gcttgtgttg atttcaattt
    27601 atcttctgtg ctaggaactt tacctgctct ttcttctact tcatcttgaa gccctgtatt
    27661 ttggtcaagc ccaaataatt ttgcatactc aagtatttta tttgcattca tatctatacc
    27721 aatgcttttt ccatctggcc atgttttacc tgtaccaatt gttgccatat atatattaca
    27781 agactcttgt attgctttat ataagtttgt cataccatgg tttcctctac catggttcca
    27841 tacatagtca ccaaaagatt ttttacctaa cattataacc ccagggtcat ttatagtaaa
    27901 gtttggatcc agaccatttt ctaacgctgc cattgaagtt accatcttaa atgaagaacc
    27961 tggctgaaat tccccttgtg taaccaagtt taaaagtgga cttcctgcta gtaagtcatt
    28021 tggatttttt ggttgaagtg ctttataatc ctctgtagaa ataccttttg caaatttatt
    28081 tgggtcataa ttaggataac ttgatgaagc taatacttct ccatttttaa catcaatagc
    28141 tataagagct gctgactgtg cttttcctgc atatgcacta attggtttgt ccccaaattt
    28201 acttttaaaa gtacctcctt tagaagcaac ttcgattatc tgttttaatg cattatctga
    28261 aacttcttgt aaatctttat ctagtgtaag atacacagta tctcctgatt taggtttttt
    28321 agattcaatt tctttactga ttctacccaa tgcatcaact ttaaccattt tataaccatc
    28381 agttcctctt aatctacttt cattactctt ttctactcct gcaagaccta ccatatctcc
    28441 agtttcataa cctttttgaa ggtatgattc tatttgagtg gatggcattt ttcctacata
    28501 tcctaacata tgagaagcta atgacccatt aggataatat cttactggtt ctactgctat
    28561 tgaaacacca acaaggtcca tagcactttc ttcaatttga gcaatcgtag tttctcccac
    28621 atccttagca atagttactg gattatattg agaatatcct tttgatttta tcaaatccct
    28681 tacgaccatt atttttcttg catcttcatc actcaaactt ttatcaattt ctaatgcatc
    28741 actatttctt actttttcaa acgcttcttt ggcactcagt ttagtttctt ttatcttata
    28801 actagccaac caatctctct tatctctttc tgctgtgaac atccatttac taactaaaat
    28861 aggtggataa tatccatttt catttagttt tgcctgtaat cttgtcgcat ccaatctttt
    28921 atcttcttga gataatattc cagctgatat aagtttgtcc accaagtaat aaaaactttc
    28981 cttagcatta taatcatttg gaataccatt ttctgattta tattccctaa tatctctgtc
    29041 atatgtataa tagtactttc cattttctac atatatagga aactcatcta catattcttc
    29101 accatttctc tccagcaaat ttataagttt taatgatatt tcatttgctc ttgaatgctt
    29161 atttgcatct ttcttattaa tatcgttacc tgaaacttga acagtaaata agtttttatt
    29221 tcctgccaac agtcttccat atctatcttt tatttctcct cttggagctt ctattgccaa
    29281 ctttttatat gttttattct cagctaactc attgtaatat tcatatttaa atgtagtcat
    29341 atagattatt tttactaaaa tagctaaaaa tatcacgccc atgactatct taattatttt
    29401 tagtctatca acttgtttat tttcttccat gaaatcacca actaatcttc tttaagtttt
    29461 aaaactgctc ttttagataa gcgatatatt actaaaccaa ttaaactatt cactattggt
    29521 gctactagta gaccttttac aaaaagtgtt gatatgttgt agctttgata aattatacta
    29581 gatgtaccta tattgacaat tgaatcaatt aaactgccta taaaaattaa aacgaaagta
    29641 ataatataac tacctttata tagctgatct ctcatgtatc caattgcata tgcaataata
    29701 aacaaaatta atgcatttac accaaaaatt ccaccaacgg ttatgtcttt aataagccct
    29761 attattgctg ctataatccc accttctaat tcatctaaat aaagagagat tatggttaca
    29821 tatataagaa ctaaattaaa gcttatgcca aaaatatcta tataatttgt tatactattt
    29881 tctatagtca taagcacaat ccccaacaga caaagtaaaa cttttttcat tgtttttatc
    29941 ccctttatcc tatatttctt ggttctatga ctacaacgtc atttatattc ttaaaatcaa
    30001 cataaggttt tacaactaca tattttaatg acttatcttt atcatcaacc actttttcaa
    30061 cttcgccaat tggtatacca tctgggaaga atccaagacc tgatgttgtt attatatctc
    30121 cttgaattac attatatgat gagtcaaaca tatatccttg caaatatcct ttactattat
    30181 aactatcttt attatctaaa gtagtatttt gagtaattgt acctttagca ttggcatcct
    30241 ttgctagttt aaagcttaca gatgccttag aatccaatag tgaaattgcc ttactataat
    30301 tgttggaaac ctcataaact atgccaacta atccactacc attcataact atactttctt
    30361 tttttacacc tgaatttttt cctgcaccta tgacaaaact ctcataccag tttccatcat
    30421 ttttaccaac aacacttgct gaaatacttg tcgccttata ctcttcttgt acaaagttca
    30481 gtgtcttttt taactcttct agcgattctg ttttatctag tttggcattt aaagctatca
    30541 tatttttttt aagtttttca ttttcttctt ctaatttttt taccttttta gaattatctt
    30601 taaaatttgc tacatcttta aaaccatttt tgataaaaga aaatccatca tttattccct
    30661 tacctacact tgtaatagca tctgaagcta ctcctaaatt tactggacta ccactagaaa
    30721 atttaccaat tgatataccc acaattccaa ttaaagtgat agcaactacg cccgttgcta
    30781 tcactttaac attaatcctt tttttctcat ttttgttctt gtcaaatctc aaggccatca
    30841 ccaacttcta attcttagta ttcatcatta atactttttc aaagatttct tggtcttcaa
    30901 cagatttacc tgttccaagt gcaacacaat caagaggtgt ttctgctatt tgtacagaca
    30961 taccagtttc ctgtgtaata agcttatcaa gacctctaag caaagcgcct cctcctgtta
    31021 gcattattcc gttttccatt atatctgagg ctaattctgg tggagttctt tctagtgtag
    31081 attttattgc atctactatt gaacttacag gttcttttaa tgcttctcta acttctgtag
    31141 agcatatttc tattgtttta ggtaatcctg agattaaatc tctacctctt atttgcatat
    31201 ttatttcttc atcatcttta aatgtagaac ctatgttaat ttttacattt tcagcagttc
    31261 tttcacctat cataagatta tattcttttt ttacataagc cactatagac tcgtcaaatt
    31321 cgtctccacc tattcttata gatttagcag taacgattcc tcctaaagat attacagcta
    31381 tttctgaagt acctccacca atatcaacta ccatattacc ttctggttct tcaactttta
    31441 atccagcccc tatagccgct gccattggct cttctataag gaatgcatct ttagctcctg
    31501 cttgtcttgc agcctcttct atagctctct tttctacttc tgtaactcca aaaggaacac
    31561 atatagctat tcttggactt acaacacctt ttttatcagc cgctttttgt ataaaatagc
    31621 taatcattga ttgagttatg tcaaaatcag ctattactcc atctttcata ggtcttatag
    31681 ctactatatt tcctggtgtt ctccctatca tttttttggc ttcttcacct actgctaata
    31741 cctctttact atcatctctt attgcgacaa ctgatggttc tctaacaaca ataccttgtc
    31801 cttttatgta aactaatgta tttgcagtcc ctaaatctat tcccatatcc tttgtcatct
    31861 tattaaaact aaaaaaacct tttttttctt tctttttttc cttagccatg attttactcc
    31921 ttcctactat taagaatcat gtttaaaaca ttctcctatt attttatcac atataaagtc
    31981 aaattatcat attttcttta aagctaaagt ataatccatc tccaataatt atatggtcta
    32041 ttatttctat tccaattatt tctccacatt taataagtct tgatgtgata tttttatctt
    32101 ctcttgatgg ttctacactt cctgaagggt gattatgcat cactattatc ttacttgcac
    32161 ttcttcttat agcttcttta aaaacctctc ttggatggac taatgacgag cttaaagtcc
    32221 ctatagatac atctacatca gatattatct cattttttgt gtttaaaaga accgctttaa
    32281 aaacctcttt ttttaaatac cttaggctat ccatataata cctctgtata tcccaaggat
    32341 tccttatttt atactttata ggtttaaaac tagctactct agagcctagt tctaaagctg
    32401 ctttaatttg ggttgatttt gataacccaa ttccatctat attacacagc tcctctattg
    32461 tcatatcttc taaatgtcta ataccctgaa tatctctatt tattatatag ttggctaatt
    32521 caatagcatt tttattcttg ttgccagttc ttaaaagtat agccaacaac tcagcatttg
    32581 ataagctttt tacgcctttg gcaagcatct tttctctggg tctttcctct ggtgccattt
    32641 cttttacctt aatggttgaa ttaaatgact ttttcagata aatcacactc cataaaaaag
    32701 atttatacta aaatattttt ttaataggtc gcctaacctt gaaattggaa gaccaactat
    32761 attaaaataa tctcctctaa tttcttcaac taacaaagct ccataaccct gtataccata
    32821 ggctcctgct ttgtcaaatg actcattagt tttcagataa tcctcaatat cctgctctga
    32881 taacgtttta aatttcacat tgctgattac atagtcaatt acctttttat cttcgcacaa
    32941 gtttattaag ctaatacctg ttatgacttg atgctctctt ccagatagtc tttttaacat
    33001 atctcttgca caagactcat cttttggttt tcctaaaata gcattgtcta aaacaacaac
    33061 tgtatcagct ccaataacaa catcttcatt atgttcagat gctacagaca tacttttttc
    33121 aaaagctaat ctcataacta aatgttttgg tgtttcacct tccaatataa tctcatcaat
    33181 ttcattttta attatatcaa atcttacatt cgtattctct agaatttctt tccttcttgg
    33241 agaggctgat gccaagatta tattcattta ttcacctcat ttgtacgtaa tttgtaccta
    33301 tttagtctat cactatattg taaaaaattc aacagaaatg tcgaaccttt tttgttacac
    33361 tattgttata tttattacca ttagaataaa tatacataaa atagaggtat ggaaattatt
    33421 ccatacctct gttttatgta ctcattagag atatggaaat tattccatat ccttattttg
    33481 tccatattac aaaattttaa tagctttctt tggacatttt tccatacata aatggcatcc
    33541 tacacattta tctgcatcta ccttatgttt ttcttttaat tctccctcta ttgcatcaaa
    33601 cttacattgt tttttgcaca ctgtacatcc aacacaaagt tcttggtcaa tagtaacttt
    33661 ctttttctta gttatgtcac ctgatattac ttttgtaggg cattttgcta cacattgcat
    33721 acaacctaca cacttattag ggtctatctt agctattttg tcttcaaata ttattgcatc
    33781 aaatttacaa gcttttacac acattccaca gccaatacat ccagcagtac atttttcttt
    33841 aacagcttta ccaaactctt tgctattaca ctctacaact acttcttgac tttctggttt
    33901 tgtgattatt atacccttag gacaaacttc tttacatttt ccacaattta cacacttttc
    33961 ttcatctatt actgctattc catcaactat agaaatagca tcaaatttac aagcatcttt
    34021 acatgttcca agacctaaac atccaaattt acacatctta gcacctgagt ttaaaacatt
    34081 tgctgctcta caatctgata ttccttcata ctcatattta tcttttgcac ttgaacaagt
    34141 tcctttacaa ataacttttg ctacttgttt ctcacctgct tcagcactta ttcccattat
    34201 ttctccaact tttgcaccaa catctgctcc acctacaggg caaccattaa ctggtgcatt
    34261 gccttcaact atcgcatttg caagacctcc acaacctgga aatccacaac ctccacagtt
    34321 ggctcctgga agtactccta gtatagcttc cactctctcg tctacttcta cagcaaactt
    34381 tttagatgca aaatctagta ctatcccgaa gattaagccc ataattccta aaactaatac
    34441 agctgtaagt atcaccattt tcacccctct tttttatagc tttataagtc ctgtaaatcc
    34501 taagaaagct attgacatta aacctgctga tataagcgtt atagggaaac ctttaaatgc
    34561 ttctggtata tcagctaatt ctagtctttc tcttatacct gcgaatatta ctattgctaa
    34621 agtaaatcca gctccggctc caaatccgtt tattattgtt tctactaaat tataactttt
    34681 ttgaacatta actagagcta taccaagaac tgcacagttt gtagttataa gaggtaaata
    34741 aactcctaat gcttgatata aagatggact catcttttga attaccattt caacgaactg
    34801 aactatagaa gctataacca atataaaagc tattgtttgt aagaatccag ttccagtttt
    34861 tactaataaa atctgtataa aataagttat aattgaagct agagttaaaa cgaacgtaac
    34921 tgcaactccc atccctactg ctgtatcaac cttcttagaa actcccaaga aaggacatat
    34981 acccaagaat tgagatgtta taacgttatt aacaagaacg atacttaaaa ataaaagtat
    35041 taaattcatt tattacacct ccactatgct tttttacttc ttaatttatt aaatcctgca
    35101 aataagaatc ctaatgttaa gaatgcacct ggtggtaata taaatattaa tactggttgg
    35161 aaagatgctc caaataatgt cattccaaat aaactaccat ttccaagtaa ttctcttact
    35221 gcacctatta ttgtaagtgc aacagtaaat ccaagacctt gacctacacc atcaactatt
    35281 gaaggcatag ctcctgtttt gaaagcaaaa ctttctgcac gagctaatat taaacagtta
    35341 acaactatta atggtatata tataccaaga gccttatcaa gagcaggaac atatgctttt
    35401 aaaagcatac ctactattgt aacaaatgta gctataacta ctataaatat aggtattctt
    35461 atcttatctg gtgtaatttt tcttattaaa gatataacta aatttgcaca tattaaaact
    35521 gctgctgttg aaagacccat accaattcca tttattgctg aagttgtaac ggctagtgtt
    35581 ggacacatac ctataacttg aacaaatgtt gggttctcat ctattagccc atttttaaat
    35641 actttagcta aattcataat taaccctcct tatttattaa gagcactatt gaaaacatct
    35701 atagcagcat ttactcctgt agtaactgct gtagatgtaa tagttgcacc tgatattgct
    35761 aatatttgat tatctgctgt tgtacttcct tttgctactt caagaggctc agcagttttt
    35821 cctttgtatt gacccttaaa tgcatcatcc ttagcttttg ctccaagacc tgctgtttct
    35881 gacatattac caatgtcaac acctgtaact tgaccttcag atgatatacc taccataact
    35941 tcaatttctc caccatatcc acttggttta gcttttaaag tatatccaac cacttcagaa
    36001 ccatctaaac cttcatacac ttcagctata agaccatctc caaggccttt gtaagtatct
    36061 tccttcattt ctttaaattc ttttgcttct ggaaggactg cttttctaag ttcattattt
    36121 gcttgtatat ttctttgttc tattactgga gctgtaacct gattagtagc tccaagagct
    36181 aatgcagcta ttgcacttat agcaagtaaa gtaccaccta atctcaccat actgttcatt
    36241 atttagccac ctccccaaat actctatttc taacaaactt atctataagt ggagcaagac
    36301 agttcactaa tagtattgag taagatacac cttctggata tccaccatac attcttataa
    36361 cagttgttat aagaccagct aatacagcgt atattatttg acctttttta gtcataggcg
    36421 aagttgtata atccgtaagc atgaagaatc cacctaacat aagtcctcca gcaaacagtt
    36481 gatatattgc gaaagttata ttaaatccac ctaaaaggaa tgttaatata aatacagttg
    36541 ctatgtaaaa tactggtata acataattta ttatgccttt atacattagg tatactccac
    36601 ctaataataa taatattgct gatgtctcac ctatacatcc accaatattc cctatcatca
    36661 tgtcagcaag actaattcca ttgctagaaa tatctgccag tcctgctgct ccatttttca
    36721 aaaagcttaa tggagttgct gtagtaactg catctaaatt tttacctatc cagttaacac
    36781 ctggttgagt ccaagcagtc attgctactg ggaatgatgc taataaaaat gctcttgcag
    36841 caagtgctgg attgacaaag ttacatccaa ttccaccaaa taccatttta actactaata
    36901 ttgcaaatat tcctcctact agacacatcc accaaggaag tgatgctggt acgttaaatg
    36961 ctaataataa tcctgttaca acagctgaaa aatctcctat agtacttttt tgcttcgtaa
    37021 atttttgata taaaaattca gtacctactg taccaagtat acaaaaaaac atagcactca
    37081 aagcattaag tctaaagaag tatactcctg ctactgctgc tggaaggagt gctataataa
    37141 cttgtttcat tatatatgaa gtatcttcat tacttctcac atgaggagaa gatgatacta
    37201 tcaacttatt ttccatcgtt tttccccctt aactttcttt atttctgagc agcttgcttt
    37261 cttctctttg ctcctatttc tctctttgct gatcttatag attgaagtaa tggtcttctt
    37321 gctggacaaa taaatgagca agacccacat tctatacaat ccattgctct atgatattca
    37381 gctgtgtcat agtcatcttt taaagaatat gcacttatga aaagtggttg taagaatgat
    37441 ggacatacat ctgtacatct tccacatctt aaacagtttt gaatatctgg agtacgtgat
    37501 tcttcctcat ccaaacataa aattcctgaa gaacctttgt tagtcgctat ttcagtagta
    37561 tattgagcta ttcccatcat aggtccaccc attataacct taccaatctt cttatcttct
    37621 ttaaatccac cacattgttc tattatttct gaaactaatg ttccaacttt tgttattagg
    37681 ttctttggct ctttgatgca actacctgtt attgtagtga ttctttcaac caaaggcata
    37741 cctgttttaa tagacttagc tatctgtgct gctgtggcaa cgttgtcaac aactgcacct
    37801 gctgctattg gtaatccacc agatggaact tctttgcctg tacacgcata aataagttgt
    37861 ttctctgctc cttgtggata tttcacttga aggccaacaa cttcgatttc gctatagtct
    37921 ttagctacat tttgaattgc ttctatagca tctggtttat tcgtttcaat tcctataaat
    37981 ccttttttga catctaatac cttcattaat gctcttaaac caaacacaac atcttctgga
    38041 ttttcaacca ttaatctgtg gtctgcagtt aagtatggtt cacattctgc accatttaat
    38101 ataactactt ctgccttgct atctggtgga ggagatactt ttacatggtt agggaatgta
    38161 gctccaccca tacctactat tcctgcctct tttatgatac ctactatatc ttctttgctc
    38221 aaatcctcta actgaccttt tggttgaaca ctctcatgta attcttcctt aaagtcattt
    38281 tcgataacta cacattgagc tgaacctcct ggtacttcat gttgttcaat agcaataact
    38341 ttacctgata ctgaagaatg aacgttacat gaaacaaaac cttgttgttc acctattttt
    38401 tgcccaaact ttacttcatc accaacttca acaataggtt tagctggtgc ccctatatgt
    38461 tgctgaagag gaatgtaaac tatctttgga gcttgagctt tttcaagtgc ctttgtgtta
    38521 gagtactctt ttctatatgg aggatgtatc cctcccttaa aagttaagag tttcattgtt
    38581 ttaccccctt atctataaaa aaacattttt tctgccttct ataagtatac tatatatatt
    38641 aaaaaatctt aacaattgtt aaaaaaatat ccgtattttt ataatttatt ttataaatca
    38701 acacctctat attaagtcaa attgaggaaa aggattgctt aattaaacaa tccttttcat
    38761 atagctttct aaagtccaaa ttttctgtat tttattttat agaatttata aacatagagt
    38821 actcctgcat ggatgataaa agcagacttt tacttacact atatttttct tcatttgctg
    38881 cttttgaagc tgtatcaatt ttttcattca cattctttac atactctatt aaattatctt
    38941 taaataaacg catttcagaa taatcaatct tttcagcttc ttttgatata ttttgtgata
    39001 ttgcctttct atttgtcaaa atagtattat atttttccat atctacatta ttttcagcat
    39061 tcgaccaaaa acttgattct tctttaaagt ttgttattaa tttatttaat tctttagata
    39121 tactctctat ataagcgtag ttacttgtat actctaaaga caacataggt gcatctaagt
    39181 gtgataaaaa tgcatctgga aacacctttc ttacactgct tatctgcttt ctaacatcat
    39241 tttcatcaaa agaggaatag gtctgtattt tttttactcc atccttctcc ataactgaaa
    39301 aaggtatgtc attattaact aatgatgtct gtattttttt caaatcatca ttattatcta
    39361 ttgcagcaac ttgaatagta tacatatccc agccttctat agtagctaat ttgacttctt
    39421 ctggctcttt agtgttattt accgcttttt ctggattagt ttcttgttta tcatctgaat
    39481 tggtttgtga tttagatttt ttcccttttt ttattgaatt taattcatca gatatatctt
    39541 tataagttat atctgatgaa ttatttagaa atggtatttt agccgataca tactccaata
    39601 tcttgctatc ttttattttt gtataagaat aatagccaat tagacacaag caagcaatcg
    39661 ttataacata tttacgtaaa tcaatcttat gtctattatt attaaatcct ctgtatatcg
    39721 tttttcttct attcattagt ttgtccctcc caaaattata ttattaataa agtatatttt
    39781 taagagtcac aatttatgaa tataaataat tatttcttct aattttttaa taagaaattg
    39841 tacttactaa acaaatcttc ttgcaccaag taatacttta gaccaatatg aactagttaa
    39901 attatccact ttaaccttat taggattagt ctgagtacca ctacaatgga taaatttact
    39961 atttcctacg tatataccta catgattagt tgttccatca ccatcagtgt caaaataaac
    40021 taaatctcct ggtgcataat ttcccataga aattgcactt ccaaaattag cctgttctct
    40081 agaaactcta ggaatagaaa cgcctacaga atgtttaaaa acatactgtg taaatccaga
    40141 acagtcaaat ttatcaggtc ctgaagctcc ccattgatat ggtattccaa taagagtata
    40201 tgcaaaatct acgattgttg aaccttttgc tgtagattta tctgaatttc cagtaaatga
    40261 tggctttgag ttactattag attgagaatt ttggttagat gatgaatttg gtttttcttt
    40321 tgtatcctca ttagtctcag aaatataact ggcactcacc catccaattg ttccactcga
    40381 taactttacc ttataccaac cattactttt agcttttaat tcaacaacat ctccaccatt
    40441 taattttcct attatagaat agcttgtccc aggaccactt cttacattta aactcacagc
    40501 agatgttacc ttaccattct tgccttcaat tgattcttcg ctagatggtt ttttatcaga
    40561 attattagta ctatttgaat tactagaatt gttcgttcca acatcttctg atgttttaga
    40621 aatatattgg ctactagccc atccagtaac tccatttgat agttttattt tataccatcc
    40681 attgctttgt tctagtaact ttactacatc tccattattt gctttaccaa ctaatgaata
    40741 gttagtacct gcaccacttc ttacattcag tctagaactc acatttactt taccatttcc
    40801 actaatagtt gtcccacttg aagttggatt attttgactt gatgtacctt cagacgaacc
    40861 acttgttgat atgtattttg cacttcccca tccaacaaca gaactcgata cccttacttt
    40921 ataccatcca ttagactttt ctaaaatctc tacagttttt cccttgtata attttgctac
    40981 cttatcgcaa tcagaacttg gtccacttct tatattcaag gcacttgctg ttactgttcc
    41041 cttttcaaga gcacttgcat ttatggagct aacagatact gctactgccc caattcctaa
    41101 agctgctata gcttttttca ctactatcac tccccccaac aaatttttat agtcataaat
    41161 tcataacatt attatccatt tttagtccct acaatttaat gataacttat tttcttatta
    41221 attgtaaaga aattataaac atatccttaa aattagtgat ttttaggaag tatttcaatc
    41281 caataattat ctgggtccgc tataaaataa attcccatac tttcattttc aaaacatatg
    41341 caatctaaat ctttatgaag tttttttgcc tcatcaaact cctcaacttc taaagccaga
    41401 tgaaattcat tgtctcctaa attatatggt ctatcccaat ctctaagcca agttaattct
    41461 aatgtatgac tagtaatgcc atctcctaaa taaactaata taaaacttcc atcttctgct
    41521 tccttgcgct ttacctcttt aagccctaaa gcttttttat aaaaatctaa acttttttct
    41581 aaattagtaa cattgaaatt gttatgacaa aaattaaact tcattttgaa atcactctcc
    41641 aatttttatt ttttttatat tatatcatta ttataaattt atttaaatca tattaagtta
    41701 aaaatttgaa tgatgatgac caattaccaa atcatggtaa attatttaaa agaatatgtc
    41761 aaaaaaagaa taagctcttt caaatgcata aaaaatttat acccaagtta tacttaatca
    41821 tgcaaattgg cagaacttat tctttaaaac tctaactttt aagtttttct ttaataatat
    41881 aatttattac tttttttcta atatcttcag cattataatc atcttctact tctatatcca
    41941 aaagttttgc tattgacctc atatcttctc tagtctttga cctatagctt gatattaatt
    42001 catcttttat tttctctggt tctaaaagat attcttcatc tcctcttttg aaaacaacgt
    42061 atttttgagc atatgaactt ctattgtagt atatatatct tgatattttt cttaaaattt
    42121 gattatatga aagtcgcttg ttaatatcaa gttttaagta ctctgaaaaa gcaaatagct
    42181 ctttttttga tttaaataat gcttctttct ttatagttct tgatattgaa gttacacttt
    42241 cttgatatgc acactttttt aacattttta aatatactct attaagacca atactttgat
    42301 tgaatttttt tatattatta ttaataatct tatccaagtt accttcaaag tatttttttg
    42361 ctcctttttg tgatacataa ctctcaagtt ctttgtgggc catatcaagt atcatttttt
    42421 ttaaatcctc tttatatccc ctagattcca tgatactccc tcccttagct atttatagta
    42481 tattcaaaaa gctcaagaaa tgtgagtcat tacaaaattt ttttactttt tatgtatata
    42541 ttaatttaaa aggggagata atagtaatgt aaaatcaaac atactcaatc tcccccattt
    42601 ttagacctct attactcccc caatagaggt ctttttttag tttatcaatt tctattcaat
    42661 tccctttagt tcaaatccta attctgctat cacttctttt aatttttcaa tagatacatc
    42721 atctttcata tctacagatg cacacttatt ttctaaatct acatctaaaa cttcaatacc
    42781 atctatatct tctgttaatg cagttttaag atggtttaca caatgaccac aactcatacc
    42841 ttctactaat agtttcttct tcatttaatt acctccttat accctataag ggtattctat
    42901 catatagttt atacttttta aatagaaatt aatcatttaa tttgattcat tcgattttat
    42961 atctaaatta gatattccct catatacatc aaaatcattt ggagtaattt taccttttaa
    43021 tctactatta cttacaatat agttgtttct cacagctaaa cttataaatg ttgcattatc
    43081 ttttatatat gtctgtacat ctttgtatgc tttttttgtt tcaatttcag acgtagaact
    43141 tgtaagtgca gacatatatc catttaactt atcatcagta tatccactac tcgctataat
    43201 actacttgca tctggtataa ttgatagttt ccatccaact aatgctaaat cataattttt
    43261 agaattaaga gcattttcca tatccttatc tgacagctga tttactgtac tatttattcc
    43321 tatagctttt aagttttcat ttattatgtt tgcagttttt attctctcac tattatctct
    43381 gtttactatt atctttaagt tcatttcaga aagacttttc tttacttcct ctttttctct
    43441 attttttttc tcttcagctt cttttttgat tctatcttct tctgcttttt tagcttttgc
    43501 ttcttcttct ggcgtaagct tctttgagtt ctttttagct gattttttct gattttttac
    43561 tttattgtca ttttgattta ctgagtttac atttgcatat tctaccttag caagacactc
    43621 tatcgcctta tctttattaa actctaaatc cttcatttca ctattatagt attttgattt
    43681 tgaattaagt ggaaaattaa ctggagtagc atcatccata taaccttcac taataattct
    43741 atctttatct atagagtgtg caattgcttt tctaaaatta acatctttga aaaaaggatt
    43801 attataatta aataatacac actcataatc tcttccttga tattttgtta tattaaactc
    43861 tttttcttga aacttactta aatcatttaa tgtaacattg gttatatcac tatcaagagc
    43921 cataaccata gatgtccttg catcttcatt aggcaccata cctactttta tgttcttcat
    43981 ggtttttgga acttcttcat agtaatcttt gtttacagat aaaaccattc cttctctttc
    44041 tgtatatgat tctattttat atttcccatt tcctatcata ttattattct tgtcatttac
    44101 atcttttgaa tctaaatgat tttgagatac aattggaaaa attaaactat caattgaaaa
    44161 agcatatcta gctttaaatt ttattgtaaa atctttatct gatttaatat tcacagaagc
    44221 tatattttta gtaaattcat tatatggact atctgcactt ttttgaatta aaccaatagt
    44281 atagtttaca tcatttgaag taatattttt tccatcatgc cactttgcat cttttagctt
    44341 tatatctata ctcattccat cctgagctat attatactcc ttaactaatt gaggaattac
    44401 attgtagttc tcatctatag taaataaact atcataaact aaattcatta tataaccaac
    44461 agacttatca gtattagtta taggatttat agtttttgga gttaccattg ttaagtttat
    44521 atacttagca tctatagaac tttgactttg taaacttgaa tcactactac tttgttttgt
    44581 tttagtatta ctacaaccag ctaccataag agttatagct aaaacgactg ttaagacttt
    44641 tattctcttc attagttttt tcctttcttg ccatatatct tattataatg ttcgtaatta
    44701 ttttttactt tttctacata ttttgaagtt tgcttaaaag gtatatcatg tagattttcg
    44761 ccatctttac tatattcatt attttctaac cattttttca cattacctga accaccatta
    44821 tatgcagcta tcactaaatc taatttacca aattctttgt ataacttact taaataccaa
    44881 cagccaagtc ttatattagt ctctgggtcg aaaatatcca cattttttaa atttagttcc
    44941 tctgctcccc aatctctagt tatgtctaat atttgcatta atccttttgc ttcctttttt
    45001 gaaacagcag aactattaaa cttactttcg gctttaataa cactgtaaac tatattttca
    45061 tctaaattaa attctttcga atacttttct acatactctg aatatttttt aggatataaa
    45121 aatttatgta ttactttgct ttccattaat agtgccccaa ataagattat aaaaatagat
    45181 agaatcaata ctttcttact attcacgcca attctccttc atgtactcaa taaatttatg
    45241 tgctttactt tctaattcag ttattgttcc cgaattatct attatataat caccatattt
    45301 ggatttttct tcctgactca tctgtgaatt tatcctacta agtgcttcct gctcactgca
    45361 attatctctc ttttgtattc tactaatttg tacttcttgt ttacaagtaa ctactaatag
    45421 cttgtcaacc atatctaaaa aaccactttc taccaatatt gccgcatcta acactactat
    45481 attttcgcct ttttttctca atttttctat ttcacttatt attttttctc ttattcgtgg
    45541 atgagtcaag ttatttaatt ccttaagttt ttcttcatca ctaaaaacta ttttcccaag
    45601 agcttttcta tttagagttc catcatcatt ttttatactt tgcccaaaat ggacaaatac
    45661 tttttccaat aacaacttat cctcaaatat cttcctggat ataatatcag catccactat
    45721 aggaatgtta agatttctaa atatatttga taggctgctt tttccacagc ctattcctcc
    45781 tgtaagtcct aatatcaaca taaaatcacc tactttgtct cataccaaga actacctaca
    45841 tttaaatcta catctaagtg aacatccata tttacagcat tttccatttc ttcttttact
    45901 attttcttaa ctatatctat ttcatcatca attgcttcaa ctatcaactc gtcgtgaact
    45961 tgtagtataa gttttgattt caaattgttc tcttccaatt ttttatgaac atttatcata
    46021 gcaattttta ttatatctgc tgcacttcct tgtattggtg cattcattgc aaatctcttt
    46081 cctctatttc tttccataaa attgcttgac tttatttctg gtatatatct tcttctgttt
    46141 aaaattgtta cagcatatcc atcctcacta gccttttcta ctgttgtgtc cataaactct
    46201 tttattttat ggtatttagc aaaataactt tcgatatatt ctttcgcctt tggaacaggt
    46261 atatttaaat catcagctag accaaagtca cttttaccat atataatacc aaagtttaca
    46321 gctttagctg cacctctcaa ttcactagta acatcttcca taggaacatt aaaaacttgt
    46381 gaagctgttt tggtatgaat atcttcatga tgtttaaatg cttctatcat attttcatcc
    46441 tgactcatat gtgcaagaac tcttagctct acttgtgagt aatcggcatc aaccaactta
    46501 cagttatcat ctgcaataaa cacttttctt atttttctac ccatttccat ttttataggt
    46561 atattttgaa gatttggttc tgtagatgaa attcttcctg tagtcgttat agtttgatta
    46621 aatgatgaat gtattctatt actttctgga tttattatat ttaaaagtcc ttcaacgtaa
    46681 gtagaattta actttactat ttgtctatac tcagttatct tatctattat tggatgctta
    46741 tctcttaatt tttctaaaac atctgcatta gttgaatatc cagttttagt ttttttaatt
    46801 attggaagct ctaatttttc aaataatata acacccaatt gttttggtga atttatattg
    46861 aatggctctc ctgataattc ataaatttcc ttttctaatg tagctattat ttctttaaat
    46921 tcaacactca attcatccaa tatacttttg tctactttta taccttcata ctccatatga
    46981 cctaagactt ctactaaagg catctctaca tgataaaaaa gaccatccat actcatatcc
    47041 attagacttc tttccatcat aggaactgtt tctttaactg tgcatattat ttgacccata
    47101 tactctgcta aatcctcaaa ttctaaatcc tcatagtttt tagatttaac accctttcct
    47161 aataattctt cttttgattt aacctttttt gataaatact tcatagctat tgcactacaa
    47221 tcatatgagg tagatgaagt tgaatctatt aagtactcag ctattgttat atcaaaatac
    47281 atattttcta agtttatatt atatggtttt aaagatatat aatcatcttt taaattatat
    47341 ccatactttt taatttcatt atttgaaaat atactatcta actttgacac ttcatttgaa
    47401 tctacataat atattttttc tccatctaca cttataaaaa tatatattat atttttttca
    47461 agtatattac cttgtcctct aacagttttt aaaataactt gtcctttttt gtttacttca
    47521 tctataaatt tttctatatc ttttaatttt aatatttcta ttttttcttc tacgtgttct
    47581 gtgtcatctg aatcgaccaa atcaactaat cttgataaga gacttgtaaa tccaaagtat
    47641 ctaaatttat ctaaaagttc atctttatca taatcgccaa aaatcatact atctaaatcc
    47701 acttctattg gcacatctct tataattgtt gcaagtcttt tactttgaat agctatttct
    47761 ttgttttcct ctattttctt ttttacacta cctttaagct tatctgtatt ctctattaga
    47821 ttctctatac tagaaaactc ttttataagc tttatacctg ttttttcacc tattccaggt
    47881 actccaggta tattatcaga cttgtctccc ataagaccct ttaaatcgat aaattgtgtt
    47941 ggagtcatct catatttttc aattacagaa ttaaagttgt attcttctac ttctccaacc
    48001 ccttttttcg ttataagggt agttgtatta tctgaagcaa gttgaatagc atctttatct
    48061 ccagttacta tgtatacctt atatccattt tcttctgctt ttttagaaac agtacctata
    48121 atatcatctg cctcataacc atctatttct agtctattta ttctaaattt atctaaaagg
    48181 tcttttaatg gctgtaattg ttctgccaat tcatctggca taccctttct tccagcctta
    48241 taatctttat attctaaatg cctaaatgta ggtgcttttc tgtcaaaagc tacacttata
    48301 tgtgttggtt tatatatatc tatcatttta aatagcattg tagtaaaccc atatatagca
    48361 ttggtcttta acccttcttt attattcatt tctggaagag cataaaaagc tctatttatt
    48421 attgaatttc catctattat aattaatttt ttttccaaat tatatcactc ctaaaaatta
    48481 tctatttttg taacatatat attatatacc aatatagtgt tatttaatat tcccataata
    48541 ataatagtat attaaaatta aatatgttgc aaacaatata tttttttgct ataataatat
    48601 acatgacatg cccgagtggt ggaattggta gacgcagtgg actcaaaatc caccggacta
    48661 atactccgtg ccggttcgag tccggcctcg ggcaccatta aaaactccta aagctattta
    48721 ttcaatagat taggagtttt tttatttttg atttaaattt taagttattt tttgaaatta
    48781 ctgtgtcttt ttaaaattta tataattata taaatttatc ttctagcatt aatttaaaaa
    48841 ctaatttact tttatataat tttctatttt atatcataac ttattacaac cttttatcta
    48901 atttattgta tacaacaaca ctacaaataa attgaaataa aataagcaat ccaagaacaa
    48961 ctaaaaccca cttgcttatt accattacag aaaatacact caatatagaa acaataaata
    49021 taagaattac aagaacatag cacatgattt gattagtaat acgtccagct ttatcacgta
    49081 acataattct tctttcatca ttcatctcta tcctttgatt ttttatcttt tcttcataat
    49141 ctactgctct ttcaggttta ctccaataca tatattgaag aatcatacat acacctggac
    49201 ctaaagcagc tccagataat ccccaaataa aagcctcaaa tgaaaactca gtaaatgtag
    49261 ccaataaaac aagtaaaatt ccacaaataa aatatataat tccaacatat aacatattct
    49321 ttttcatttt tactcctcct cataaataaa tatttcttca atactcatat caaagtatct
    49381 agctatctta aaggctaaga tgatagaagg attataccta ccattttcta atgaacctat
    49441 agtttgtctt gatacttgca aaatagaagc aagttcttct tgttttatgc ctctttcttt
    49501 tcttatttct tctaatctat ttttcacatc tatccccctt taatatctca actacggaaa
    49561 gtctactttc cataattata caactatatt ccatttatgt aaagcttact ttccatattt
    49621 acaaaaaaat aacagactat tttcagcctg taattttaga atttcatgtt gtttattaat
    49681 tttaatgtta tttattaatt ccatattatt tattaatttt aacttggttt ctgaattgga
    49741 atccaaactt ctgtagtata atcaggagca ttgatatcgc cattgggata aagttcaata
    49801 tctggtaaat tagcatacac atacccagaa cttggtaacc actcagtaat aattcttttt
    49861 tgcaattcct gtattgccag agcatttggc attggagaaa cacattcaaa tactgcccat
    49921 gtgcctgatg gtattaagta ttcatgagta tcttctaata caggcttatc tgttggagca
    49981 gcaatataat aatctaaacc cttactattt gtacacacac taactccaag caatccacaa
    50041 ggttcattat ttattaactc tgaaagttta tcaattgttc cattttttat gcatttagcc
    50101 cacaacttag gaactttctc aaagcactct tctacaatca tgttcatact ttcttttata
    50161 ccaacaatac gaaacccttt cttttctaca atacgatatt gcatttctat atttcctttt
    50221 acataaatgc ttaatgtcat cttaggatat gcttttaaaa caacaccctt tgctctagct
    50281 gcagatggag aaatgttatg tatattttga aaagctctat taaaagatgt tggtgaatcg
    50341 taaccatact tctcagatag ttctaaaatt ttgatgttac tgttctgaag ttcaaatgca
    50401 gccttagtca tacgtcttcg tctaatatat tcagataatg gtacttctgc aatatatgaa
    50461 aacattcttt gaaaatgaaa ggttgaacaa catgcaattt ttgctgcttc tgcataatcc
    50521 actccacttt cgagattttc ttcaatataa gtaatagctt gatttaattt atttatccat
    50581 tccatttgat attacctcca agcaaattat acagtttaat aaaataatat acctctctat
    50641 tcatgcacaa aattgtaagc atttaaattt ataagcattt ttcaatcaat acaataaata
    50701 taaaaattgt attatacaat aaaaaagttc aataacatta tattgtaaaa tgttattgaa
    50761 ctttttataa aaattaattt aagtgtagtt aaattaagta ttacactatc ttatatatta
    50821 caatatccta ctatcttata gtcttctggc actttataat ctttgtctgg agtacctaaa
    50881 ttccatttta aatcaaacat agttgcactt attattgttg caaaataaag cataactata
    50941 ccaatgtcta ttttttcttc atataaatta gtatgaccat cttttctaac agctaaaact
    51001 acagtgcctc catcaactat aaatctccat ggttgtctat ttaatgtaga tggagctaat
    51061 cttgcatagt gaaatgcatc aagaagtgct ctttcttcta agatttctat acttgcaaca
    51121 tttccccatt cgttcatata aacaacatct tctacaccta gtctagtaga ccccttatct
    51181 gttacaactt gcatatcaga ttttgaataa ttttgcccag tttttgtagc atttaatact
    51241 ttagcattac ttacattttc tccatatcca atagctataa ttcctgttac ctctttatct
    51301 gaattaattc caagtttttc ttttaccttt tttccatctt caaaagtaac ccaacaagaa
    51361 ccaattccaa gttctgttac tttaaagatt atactttctc ctatgtaccc tgaattttca
    51421 atgtaattat cttttatgtc agataatatt ataatataac ttggagaatc tatcatattt
    51481 cctttgtacc ctgctacttt atctaaatta ttaaaaactt cacttctatt cataattctt
    51541 acatcaacat ctatttctgg aactagtttc ttacaagatt ctgagtattt cttaatttca
    51601 ctaagatatt cttctttaat atctgtgttc ttatattctc taacagactt tctatttaat
    51661 atgattgttt tataatccat aattttatcc tccccaaact agtattgtct taattatata
    51721 cccattaata tattatttta ctcatttatt taataaataa agattacaat tttgtaatta
    51781 ttatttatta ataatattcc acttttacat taataatgac cagttgtgca aaatccattg
    51841 ccaaccacca aataaaaaca tattataaag gtaatataaa ataacaaaca aatcttaaca
    51901 aaaaattaaa ctttttttac acgaaagaag gcttcccgtt gccttcttta tttttataaa
    51961 aaaataagct gtttctaaaa tgatttcaca tttttgaaac agccaagtta ttctataatg
    52021 acaactataa tttgaggatg tgagggagag ttgtcattat aaatttaggg taattatatt
    52081 atgttttaag ctagttcttt ctatatttat ataatacatt ttatatatga attttatatg
    52141 agattggtat aaatgatata tgaaattaca tatgtttata ttatattaaa ttttattatt
    52201 acattttcca caacaatact catatgtctc cacattattt ggaatattta caaatacaaa
    52261 ttctttacag ttctcacaat actttaatat agatatagtt ccacaagtgt aacattcaaa
    52321 tattacacaa tcctcatttt cctctactat ttcaaacttt ttactatgtt caccacactc
    52381 tgggcacttg tgattattta cattcataaa tactcctcct tattagtata gttacatgtc
    52441 aaaaacttaa ttaatgtatt ctatattact tattatatat tctataataa atcatattct
    52501 aatataataa aacaaattaa ataaaaaaaa aaccataaat tttaaattta tggattaatt
    52561 cttgtaatga taactctatt tgaggatgtg agggagggtt atcattacaa tagggtatta
    52621 tattatggtt tttaagctag tctttctaca cctcatatga tacatatgct atatgaattt
    52681 tatatgattt ttacataaat attttatgaa atttaaattt tttagtcatt ttgaaatttg
    52741 taaaaagttc tctaaaaatc cacagcatca taaacagcca aaatttaagt aaaacatcat
    52801 aaaaaaattt gtccaataat tttaacaagt aatctcacct cattataatt attgacacaa
    52861 acttaaataa acaatctata aatcttctat aaatcttaaa ctttccccct attgatacac
    52921 aatttctagt ttgtcaattt tattttgcat ttctaagaaa atttcttcaa cagaattgtt
    52981 aaatatattg cctaaataga ttcttttatc tttttgaaat gaataagatt ttacactacc
    53041 atcagaagaa atatataaat gactccactc tgctccatat tttgaaaacc tctcctttgt
    53101 atctttaaac ctcatatctt ctatttttat tgctttttct ctgtctcctc ttactacagt
    53161 gtgacccctt tttattaaac tggttatttt tatatcttct tttaatttaa tacgtgtatc
    53221 atcatttttt gaatatactt tccatatcac tttatgacga taattatcta attcctttat
    53281 taagcttcta tcaatagtaa aactatcttt agttaccaca attaaatcaa aatggtctaa
    53341 catatattga aatatttctc tcatactttt attttttaaa atatcctcac caataacctc
    53401 tataaatcta cagcctttct catacatgca gttcaataaa cttgtgattt cagaaaaata
    53461 atcatttgta ttattatctc catctatttc tatagtaaag tacaaaggag taatgtatga
    53521 tttatttcct gttatctcaa atacacttga tagtgtctgt atatcatctt caaacgttaa
    53581 gtacacattc tcataaaaca taaaaaattc cataactttt tctttcatat tatggatatt
    53641 tctatatcta ttggataata tatttattat atcgtcagta gtatttttcc catcacattc
    53701 agccagtata acaatttgct tccaacttaa gctatccaca tttccgatac tttttttata
    53761 tccatcttct tcgtcataaa taggaccaat tataatgttt tcttttggcc acagatgaac
    53821 cctagttcca tcttttaatt tcggatattt attttttaca gactcataaa atctacttgt
    53881 atcacccatg attctttact accccccata gtttaaatat tctatgctaa aaatagcact
    53941 ttatttttat taattcaaca attattcaaa attggtcatt atatatgttt aaatggattt
    54001 aacatcaacc ttatttatat gttattgatg tttatatatg ttatataaaa tcttatttct
    54061 catacttaaa tgtaatctta actatctctg gtttacaatt aagcctaatt aaaacttttg
    54121 agcctcctaa gcctttattt aaaatcatag tgctattatc tttttcatat tttccccagt
    54181 catacttagg aaagaactct ctatttggag ataatacacc tccaacaaaa ggtattctta
    54241 ttatacctcc atgaacatgt cctgctaaaa ccaaatctgc tccccatttt gcatattttt
    54301 caaagaaaaa tggtgtatgt gctaaaagaa tgttatattc attttcatta actttaccta
    54361 gattgtcttc tacaaaattg tcacttagtt ctaatttttt gttattagca aataaatatg
    54421 gatagcattc caatggtata attaaaccat ataaattaag atggctatta cctctatata
    54481 ttcttacaga ttcattttcc atatttatta ttttctttga atatagttcc ttaaaataat
    54541 ccttatatag atgtttatac ttttttatta aagatttttg ttcatgattt ccaattatat
    54601 gatatacttc atatttttca accaaattat ctattaaatc taatgcaact tgaaaatttt
    54661 tattttctcc atctactaaa tctcctgtta taaatataac atctgggttt aatgattcaa
    54721 ttttatctat caatacctta ttatttttat caaacacctt attatgtaaa tcactcactt
    54781 gaactatttt aaagttatca aattcctttg gaattttttt gttgaaaata gtatattctg
    54841 tcgtttctat atgctgagtt tcatacttat acagaatata caataatatt aatactaata
    54901 ctgtcattga aacctttata atcatattct cttcctcttt attctatttt gttaatatta
    54961 ttataatgct tttatgtttt ttttaaaaca ataacatcat cacatacata taaatctttt
    55021 cgacattata aaaataagtt atccttttct aaaattttca tttattttag aaaaggataa
    55081 ctataaaatt atctataaac ctctatattg tgaaatatag tttttccttt atttaaattt
    55141 ttatatatat gctctaaagt tccatcaaac ctaatagaat gtccagactc taaaatataa
    55201 gttttcccat ccacattaag gtcaagttta ccttctatta cgagcacata ttcttctaaa
    55261 acatgactat gtgtagatga aacatgagta cagttttcct caagctcaat tataaaaagt
    55321 tcaaaccttc tttgagggct aaaagggtat attggatata acttcatgtt ccctttttgc
    55381 tcaacaatag gctctacttc ttctttcttt ataacctcat aggtatttgt attttcattt
    55441 aatagtgtag taaaatttat tttaaatcca gatgaaattt tccataaagt agatactgtc
    55501 ggacttgatt cacctctctc aatctgtcca agcattgctt tactgacacc tgtcatcttt
    55561 gaagctttgt ctaaacttat gcccatttct tgtctcaata accttaaatt ttctcccaca
    55621 ttaggaaatt tattatccat gttaaaatcc tcctgatagc tatttgtatt ctatgatttt
    55681 tactcctgta agttctacta tctcttttaa ttttaacata tgattgttac aaatatcatc
    55741 aaattcatat atataatctt caagccctaa tgccttatat ttatcgattc caaatttatg
    55801 atatggaagt aactccatct tgcctcctgg aacattttgt ttgacaaata atgcagtatt
    55861 tcttatattt tcttcactat catttactcc ataaataatt ggcactctta taaccataga
    55921 tttattcaac tttgaaagcc tacatatatt atctaatata attttattac ttactcctgt
    55981 atattcttta tgaatattat catccataga ttttatatct acaaatatgt ggtctatttt
    56041 ctcaaataca tcatttacct tattccaatc aaaatatcca caagtttcaa ttgcaatatt
    56101 tatgccttta tcataaaaaa tatcaactaa ttctcttaaa aaatcaatct gaagtgtagg
    56161 ttcacctcca gaaaaagtta tccctccatt agattcaaag aaaaatataa aatctttttc
    56221 aacttcctct actatttctt caacactcat ttcttcggtc attatacaaa tggcatcttt
    56281 aagacatacc ttgatacact ctccacataa atcacattta ttatttattt gcgctctatc
    56341 aaatagtgaa cttatatttt gaggacaaac atctatacat ctattacagg atacacattt
    56401 atctttcata acacctaatt ttactatgtt actccaggaa tctggattag aacaccactt
    56461 acattttaat ttacatcctt ctaaaaatat agtagtcctt attccatctc catcatttac
    56521 tgaaaaatgt tgtactttta caactctacc ttttttactc ataatctacc tcataattaa
    56581 aaattgctat gtgaatttct agctattata gcatcttgca tttctcttga taaattaaca
    56641 aattgagtac tatatccagc aaccctcact aataaatctt tatattcttc tggattttct
    56701 tgagctttta atagagtatc atttgatatc gtattaaatt gtacatggaa tcctcctaaa
    56761 gcaaaatatg atttaatcat agatgttaga tttctaagtc ctctttcagt ttccaccaaa
    56821 tcttgattca gtcttaaatt tagaagtgtt ccaccagtat gaacatcatg atttattttt
    56881 gatgcagatt tcattgctgc tagtggactt gttgtatcag taccaacaaa tggagaaact
    56941 ccctctgtca aaggttttgt cgcttttctt ccacaaggag ttgcacctac tatttttcca
    57001 gttggtacat agtttgatat acccataaat gcactattaa atttagagcc aaaaatatcc
    57061 ttgtattttc tagtttcagt ataataaaaa tcagaaactt caattgctaa tgaatctaca
    57121 taatcattat catttccata cttaggaaca tccaacgcaa gttttcttaa ttcttcataa
    57181 ccttcccaat cattatttag tgcttttgtt aattcttcta acgtagtcac cttatcctca
    57241 aatactaatt tttttatagc tgccaatgaa tttgaaacta cacctaaccc tataccagtt
    57301 aatacaggac ctatattata ttttgctcct cctttactta aatcaactcc cttatccaaa
    57361 catccatcta cacaagtaga taaaaatggt cttggtacca tttctttatg aatttgttga
    57421 gcaacagtag ttcctataac agaacttttt acaagatatg ctaattgctt taaaaactca
    57481 tccttaagtt cttcaaaaga agtaaattct gtgatatttt ttgtatctaa ccccattttt
    57541 tctccagtta atctactctt accttcattc atagcatatt ctaaagctgc tgcaaaattg
    57601 atatttacgg ctgaagtcca ctgtcctgtt tttctaaaat gaggaactac acaaccacag
    57661 ttactccaat ctctagcatc ttctgcatca tatccatctt gtaataacat ttgtgcccct
    57721 gcactatcgc tgtgtatagc agggaaacca gttccttgtt ttaccaattt agcaactgcc
    57781 attacgaaat tatctggtgc tccttgactc actctaacac ttaatcctgg ttggtgagtt
    57841 tttacacttt ctgtagcctt taaacacata tatgagatat cattcgttcc atcagtacca
    57901 tttctctttt taccaccaac agtaagattt tggaattgat tatatcctgc aaagtaattt
    57961 gctgtatttg ctgatatagt ccatacccat tctgataact taatccaaag tgcttctatc
    58021 aattcttgag cttctgattc agttatttta ccttctctag catcattttc ataatatgga
    58081 tacatatatt ggtcaaatct acctaaattt aatgccaatg gattttctga tagaatacca
    58141 cctaattgaa caaaccatac aaattgtatt gcttcataaa aattagttgg agggtttgct
    58201 ggtacattca tacatatctc tgaaattttt ataagttcta actttctctt ttcatctgtt
    58261 tcttgcttac tcatctcttt tgcaagattt gaatatcttt gtgccaatgt cataataccc
    58321 tgtgcaacta tagaaacaga tttgtaaaaa tctatctttt caatattttc tgaaactgat
    58381 atatctaatt ctttaatttt tttatctgca tcttccttaa tccctttata tccttttaca
    58441 aacaatatat cttgataatc tggtgttact tcaccaactg cctgtctcca cttagaatca
    58501 ttatctatta ttcctgtatc tactaatatc ttagctgtat cttctgggat tcttgctaag
    58561 aaaacttctt ccaaagattt tcccttccaa tatggaaata tttcttttct tataaattct
    58621 atttgttctt ttgatattac atatgggtct tgagttcttt tatcaaaagt atccatttct
    58681 ttgtctaccc attgccaaga aaactctggt gtcaatatcc ctgttcttct aaattttcct
    58741 gctgtaccta ctattaattc attttcaaag atttttatag gaagttcttt acatgtctcc
    58801 aaaaaagctt tagctcttct tatacagata gcttctccct cagttttttt atgtgattct
    58861 gtaaaaattc tagccctttc acaacatatc ataggtttag aattaatata ttcttgtcta
    58921 aatgcttcta ttctattagt ttgactcatt taaaatcccc ctttggataa tatatactac
    58981 atttttgtat gttataacat actttaatta tattatactg tcctttagca taaaatggaa
    59041 gcaaaataac ttattttttt tcatctacct tgaacaccta tatattaata ctttttttgt
    59101 taaatttata aactctaaaa aaattttcta tgatatattt tctatattgt tatagttatt
    59161 tctaatatac taatgatttg aaatatcaaa tttgtatact agacttagtt ttaaaacctt
    59221 agttgattgt ttaactttgt taacttcaac atgtatatcc ttcatataag gaagctctac
    59281 aacacctact cccttattaa agtcttcttc aaccatagaa aaaatttctt tctcagttaa
    59341 gttcgtatta gtcaatgact taaataaatt gtatataaat ttaatatatt tatcactctc
    59401 agataactta tcatcttctg gatactcttt agcacagaga atatctattg ttacttttcc
    59461 ttctttatcg tcttcagaca tagaatagaa taaacttcga atattttttt caggtggttt
    59521 ttcagcacca tttatagata ctacaaagct accatctgat tttgagtcag taatactata
    59581 accactgtct attatagctt tcgaaaatct ttgagataaa tcttttttta cttcttctct
    59641 acctttcgca tcattaatct tttgtgagta attattaaaa agctcgatag attcatcttt
    59701 tttatcttca acttgttctt tcaacgaatc tttactaatt tgttctgagc tacttaagtt
    59761 cttatcatat acaggagaca tacaccctac catcaatgct aagctaagat ttaaagccac
    59821 aatgattgcc actctattta tattcctttt tactttcata cacaatcctc ccatcacact
    59881 aaccgctcta aagtattact tattgcacta gaatatgttt atattttctt tatattaaag
    59941 atttattatt caatacatat caccttccta tccaattctt ataacttact attagtaaaa
    60001 ttataagctc cttaatacag aaataaggat gttatctttt gttattaaaa gataacatcc
    60061 ttattttttt gtatcaatac atatgtccca actggtaact taatcgcccc aactgtcagt
    60121 atcttggtcc aatggtatat atatatttag tataaattta tcgatactat tttcaaataa
    60181 taattctcca ttatattttt gtaaagaaga tttaatactc tgaataccta ttccatgtac
    60241 aaacttatcc attttgtttg ttaacaatat atttttatta aaagtaagct gatttagttt
    60301 actattttca catttaagta caaaaaatga ccttgtcata gttcctttaa ttctaatata
    60361 tttattaaca ttttcatcca ctattttatc acatgcttct attgcattat ccaaaatatt
    60421 agaaaatatg cttgacacat ctataggttt tacaaaattt acttttgaaa aatttataga
    60481 gcaagtaaat ttaattcctt tttttaaaca tatttcactc ttttcgttga ttataatgtc
    60541 taatatcata tttcctgtat tatagatata tttaaatgtt tttatttcat cttttaaatt
    60601 gttaacatat tcattaattt ctttgctatt gtttctaagg ttgtctatac aataaatgtg
    60661 attatttata tcgtgatata gtttcttaac tcgcatatga gattcttgaa tacttaaata
    60721 atgcgcatat tgcatatcta acttattttt tattaattca gcttgagatt ttaatctatt
    60781 ctcttttatt atttctttaa acaatagtat aagaaatata ctactagctc ctaccaattt
    60841 tggattaaca aaattatcaa aatagtatcc ttctgaatgt aatgacccaa accgttctaa
    60901 tttttttaat acataattta caagcatatt cattgtatta actaataagg caaataataa
    60961 atatacataa tttctcttat cttggctaat taattttata gacttcctaa aaaagctaaa
    61021 tataagatat ataagaataa ttattaaatt actatatatc tgaattttta tagattctaa
    61081 gtgaggtact aataaggatt ctttagatga aataacatta cctgtaattg caaaaaatag
    61141 tattctatat ataacagcat ccaaacattt ataatataca ctaaatagat aaaagaaaat
    61201 ggaatactta atataaccca tcttataaaa ataattatag tatcctcccc aagcaataaa
    61261 tgctaatata taaaataaat agtttatatt actaaagtaa tttcctccat taaataatct
    61321 acttatctga attataatat aaataagaaa caagacaata tagaatacta ttttttcatt
    61381 ttttttacta tcctttttat aaattaaaat tccgttcatt atagaaaact gtataaatat
    61441 agataaaact actaatagct cagtcataaa gtttagcata ttgtatcacc aagaaatttt
    61501 aagaatttct ccttaacttc tttatatcta tatctactaa taggtacctc ttctccactt
    61561 tccagtattg cagtattagg ctttatattt tcaacatatc ttaaatttac tataaaactt
    61621 ttatggcatc ttataaattt atatggattt aattcttttt ctattttgcc taaactatat
    61681 tttatatcaa aattcttgtt tattgtgtgt attagcatat cttttttttg aacttctata
    61741 tattttattt catttaaata aatcttatat gtattagact tattttttat tgtaatatga
    61801 ctttccttat ttatttcaat atctttgata caagttagca catgtttttt tagttcttcc
    61861 aactctattg gctttagtaa atacctataa gctcttacct cataaccttc ttgcacataa
    61921 tctattaatg aagttacaaa tattatttcc atttcattgt ctacttctct aatctttctt
    61981 gcagtatcca ttccatttaa tttttccatt tttatatcaa gaagtaatat atctatatct
    62041 tttaaattac tttccagtaa ctcttctcca gatatatact tttgtatctc atattctata
    62101 cttatatctt ttaatatatt ctcaaggtaa tcttctatta aaagttgtgt ttctttttca
    62161 tcttcacaaa ctgctatctt aatcataaaa tgcctcctta cataatttct agttaataca
    62221 attaaaatat ttataatata tttaaagata attttgtata tctgatactt taggataaca
    62281 ttttcaaatt atttttatca atactcaatc ccatataact attatttgat atttcaattt
    62341 aaagacagaa attactataa ttaaaaacag tacttactga taaaagaaga tgtcttaaac
    62401 tattaaaaaa ataattttag gacaccttct tttatttatt taaacttttt aagccttaaa
    62461 taatacatta atctcctata taattgtact gcatataaaa tcaacaatat aatctgtagt
    62521 acaatagcag ttacagatgc tccataacca ctatataaat ttcttatttc aatattcaat
    62581 aaatatataa atattactac taacataaag aatataaaca tattcataaa taaaattgac
    62641 tttactttgt tttttttatc agaatcacta tataatttag gaggtatgtc tttgtattct
    62701 ttcgaccata tataccatgc agatgtaccc ataaaactac caatactcct aaatacaaga
    62761 ttccagccat ttgatttatg ttcatcaaaa taattattct ctacatcata gtagtaatca
    62821 acagtgtatt ttatttttct tggacttccc ttatgaaaat aaaatattcc tccaaattta
    62881 tttatcttat ataaattata tcctttagct tcaatatctt ctaaccattt ctctatctta
    62941 tcaggtgaac tataccagtc aaatttgaat tttattttct tttccatagc actcattctc
    63001 caaaatgata tttttaaata gcttaaaaat ctatttaaat aattatatca ttttgatact
    63061 aattttaggt tacaatcata tatttaaaaa gctatttaaa tagtaataaa ctaatagcca
    63121 taacacccat tcctgcaatc aatccataaa tagataaatg atgttccccg tactctctag
    63181 ctgatggcaa taattcatct agtgatatga ataccataat tcctcctaca attgcaaata
    63241 caattccaag agttatatca tttaaaaaat ttctaagaag tacatatcca ataatcgctc
    63301 ctaaaggctc agacatacca gataaaaagg aataataaaa agctttcttt ttatctcctg
    63361 tggcataata aactggtaca gatacagata taccttctgg tatattgtgt attgcaattg
    63421 caattgctat cggaatagct attgtaacat catcaagagc tgatataaat gtagctaatc
    63481 cttcaggaaa attgtgaatt gctatagcca ttgctgtaaa tatacctgtt ctcaaaagag
    63541 acttattttt tttgatattc ttttcttcta tattctccat tttctttatt tcatgtggat
    63601 tttcttcttg tggaattagc ttatcaataa cagcaatgat agccatacct ataaaaaaag
    63661 ctattacagt tgaccaactt cctaatttct ctcccataga ttttgtcaaa gcatcttgtg
    63721 ctttaggaaa aatttctatc atagaaacat aaatcataac accagcagaa aatcctaagc
    63781 ttatagaaag aaacttagta tttgtttttt tagcaaaaaa tgctatacaa cttcctatac
    63841 cagtagataa accagccaat aatgttatca aaaatgcaaa tattacatta ttcaaataaa
    63901 tcaccttctt tttaattgat attgattatc attttcttta tgctatcata tatattttat
    63961 atagtcaata tttataaaaa atatacacaa taaaaaagca gagatataaa cttagtctct
    64021 gctttttctt tatagtatat taaaattatt tatttaagaa atccactgca tattgcacat
    64081 ataaagcagt tccaatttct agagcatctt catctatatt aaagcatcca tgatgatgag
    64141 gataacaagc accttttgac tcatttctag ctcctacaaa tgctagtgca ccaggtgcta
    64201 tattcataaa ctcagctaaa tcttctgctc ctgttacttt ttcaaatagt gttatacagt
    64261 cttctccaaa tagcttaatt gctgcatctg tagctatctt tgaacattct ttgtcattta
    64321 taactgctgg tgttaagtac ccatactcta attcagcatc tgctctatat gcttctgctg
    64381 tagattttgc tatacgttct aaaattccag gaatttgctt tctaagctct ggattaaata
    64441 gccttattgt accttctaag acagcttcac tcgcaattac gttaaatcta gttccagaat
    64501 ttaaaactcc aacactcaca actaatggtt ccagaggact tacttctcta ctaaccatag
    64561 attgtagatt cataactatt gctgaacttg caagcactgc atcaacacct tgatgaggaa
    64621 gtgaaccatg accacctcta ccttttacag taattttaaa gaaatcagca gaagccattc
    64681 ttggtccttc ttctaccgat atggtcccag attctacatc agtccaaaga tgtattccaa
    64741 acacactatc tacaccttcc atagcaccat cttgaatcat agctcttgca cctttaccaa
    64801 cttcttctcc tggctgaaaa aataatttta cagttccatt gatactatcc ttaatatcat
    64861 ttaacacttt tgctgcccct agaagcattg atgtgtgacc atcatgacca caagcatgca
    64921 taagtccttc atttttagat ttgtattcta catcagtgca ttcaacaact tgtagagcat
    64981 ccatatcacc cctaagtgct acagtctttc ctggattagc tccttttata gttgcaatta
    65041 cacctgtacc tccagctgat acatatggta tccctatttt atcaagttct tctttaactc
    65101 tctttgatgt tctcacttct tccatacttt tctcaggatt ttcatgaaat tcccttctaa
    65161 gcttaattac atagtcttta taactctttg ttatttcttt aatatccatt ccatacctcc
    65221 aagtagtaca cattcttctt atttaaaata ctttttatca cttaaatact atattagatt
    65281 tacaaagata cctgctatta ctacagaagc tattgtaact gtagtaaatc ctccaacaag
    65341 catctttgga agcatcttat ccattaaaaa ttctttttct tctggtgttt cagccaaagc
    65401 ctttactgcc tcttcagtta atatatagtt tggtggaaaa ccataaagtg atgtaagcga
    65461 aactgaaaaa gccatttctt tactttcacc taaaagctta cctactataa tagagaatat
    65521 tcccatacct attacaccaa atattatgat tcctacaaga ggaactgcca tttcaacaag
    65581 cattgctggt gtagcttttg ctaaccctgc aaatataaaa gccatcaatg atgtcattaa
    65641 ccatccaaat gaaccagatt tattaagtgg ttttctatca actattccaa gttctgatgc
    65701 tattacacca aatattaaac atataacata tcttgataca acttcatttg taagatttgt
    65761 aaattgtact gctaaccatg ctacaaatcc taatctaagt attatcatat atgtcgtatc
    65821 atatttttct ggtaagtctg gtattatctt aaatctgctt ttttgttcag gtaaatcctt
    65881 aactttttct tcatctgtat ttacagttac ttttccactt ctaaagtctg ataaaagtct
    65941 ttttccttct tttttaagca ttaatgctgt aataggataa cccgcaaatc cttgtgcaac
    66001 atacatcaaa attgcaagta ctgataaact tgcaagacct tttgctgtag cagcatctgc
    66061 catcattata gatgcaacta gccctccagt aagaggaggt gtagctatta ctgctgtatc
    66121 ccatccaaat actactgtcc ctaatgccaa agcacctaca catattccta ctattcctgc
    66181 caaggctatt actattgttt tccattgacc tgctaattcc ttaatactca tcatagttcc
    66241 catatgagtt attaacaaaa gcatacctaa ttgagctaat ggtgttccta aagcggctaa
    66301 gtccacaata ttttctggga aaaaagtcca aaatcctact ataaatataa ttgctgatac
    66361 aaaaaccgac ggaacaaaag ctttagtttt tgctgaaact atatctccta tcgtcagtga
    66421 tactaaaact agacaaaaag ctgttattgc ctccatacaa aatcccccct cataataaat
    66481 atatttttat aaaatgtatg tatagttatc cattatttta ctacttttga aaaacatttc
    66541 aagtcaaaat tcgatttttt tcataaaatt cgataaattc cataaattaa agttagtttt
    66601 ctaaattatt ctttttgtaa aaacgttatc ccgaccattg gctatttaac tctattctta
    66661 caaagagaat taaatgtaat ttgcaaaaaa taaaacatag gatacatttt attcataaaa
    66721 catcttataa atataaaatg aattgtcttt atgaatctta atatatccta tgtttgtaag
    66781 tttaatgtgt atgttagcta tggtgtaaaa tttgtatact agttattgtg taacctacta
    66841 tatactccat ttttgtttac tagctcttca tgagtacctc tttcttcaat tcctttatcc
    66901 gtcaatacta tgatttcatc agcatttttt atagtagtca atctatgtgc aacaactaaa
    66961 tttgttctat ctttacttaa ttcctctaaa gactcttgaa tttctctttc tgttacattg
    67021 tctaaagcag atgtagcctc atccaatatt attattggag gattctttaa aaagattctt
    67081 gaaattgaaa ttctttgttt ttgcccacct gataatttta cgcctctttc tcctatataa
    67141 gtatcatatc catctggcaa agtctctaca aagtcatgta ttctagcttt tttagcagca
    67201 gctatcattt cttcatctgt ggcattaggg ttcccacaaa gaatattatc tcttatagtt
    67261 cctgtaaata aaaatacatc ttgttgaact atacctatat tttttctcaa tgattttaaa
    67321 ctaacatctt ttatactctt tccatcaatt tttatatcac ccttatcaaa ttcataaaat
    67381 cttggcaata aattacataa tgtagtcttt cctcctccag aaggtccaac taatgctata
    67441 gtcttaccag cttttataga taaacttaaa ttatccaaaa cttgagtctt atcttcatat
    67501 gtgaaagata tattttctat ctcaatgtca ccttttacat tttcaagttc aataggattt
    67561 ttagcttctt tttgatggtc ttgttccatt atctctatga atcttttaaa acctgtcata
    67621 ccattttgat attgctctgt aaagtttatt aattttttca taggttgtat aaacattttt
    67681 acatatagta tataagcagc aaaatcaccc aaagtaatct ttcctatata agtaaaataa
    67741 ccaccagcta ttacaactac ccactctaag atatccatgt ataaattcat accagagtaa
    67801 tattctgcca ttaccttata agaatcttgt cttgctcttc taaataattt attgcttctt
    67861 acaaacttat ttaactcttc tttctcagta caaaaagact tagttacctt catacctgct
    67921 atactatttt ctaaagtagc atttacatca cctatagtaa ctctactctt cataaaagca
    67981 atattcatct tatctttttt agctattgca aaccaaatta taaacggtaa tatagcaaat
    68041 attataagcg taagaggaat atttatatct ataagtataa agaatgaacc aaccaacatt
    68101 actatagaaa tgaataaatc ttcaggccca tgatgtgcaa gttctgttat atccattaaa
    68161 tcatttacta ttctagacat agttactcca gatttgttat tatcaaaata cgtatttgga
    68221 agtctttgta agtgtgtaaa tacctcattt ctcatatcag cttgcatatc tacaccaata
    68281 acatgtcccc aatactgcat aaaatagtta agccctgctt ttgctataaa tataagtata
    68341 agtgctactg caaaaactac taacattctt aagtttttat ttggaacaac gtcatccatt
    68401 atatttctag ttatcatagg ataaactaaa tcacataagg aaaatagaaa agctgctata
    68461 aggtctagtg taaaaatttt cttatatggc ttataatatt tgacaaattg ttttatcata
    68521 agtattaaat ccctccttta ttagtacttc tattagtact tctaaaaaat acccataaat
    68581 tagtataaca taacaataac tttaaatctt ttataaactt acaaagtaat aacaatcttt
    68641 ttcataaaat tgaagattta tatcatatag ttgtatactt gaaacaacat aaaaagagct
    68701 atatctctat atttaaaatt ttataaagat acaactctat atttaatact aaattttttt
    68761 tataaataca aaactacttc aatgcattta atatcttttt atctaatgct gttgtattta
    68821 tacctgttat atatcctgtt gcctttattg taggtatatc ataagttgtt ggtagtatag
    68881 tagtagatac gattaaatca gcaccttctt gttttgttga acattcagca attttacatt
    68941 gctcaacttc tgcttgtata ttattttctt taaccaatct ttctactctg tcacaaacta
    69001 ttgttgatgt tgcgattcct gctccacagg ctactaatat ttttttcata tattgtcctc
    69061 ctacaatttc tttaatattt tataactcaa aataaaattg ataaatttct tatatcttta
    69121 tttatataaa taattatatc attttatata agaagaacat cttaaaatta ttcttttaat
    69181 atgtttttta tttcaacatt gcttatactt aaaatttcat tttttataca agactctatg
    69241 tactttgata tatttttata atcatttaaa ttaaactgtt caatattcaa tttatattct
    69301 atatcactaa caatacctat caaatcagac ttattagata aaatatcctc atacttacct
    69361 tttctagtta cttccagtct tttaaatcta taatttttat atccttctat gataatcaag
    69421 tccatatctt catataattg aatcatttta tataatggat tcttctcttt tagttcttca
    69481 atatatgcta ctcgtttatc tgatattata cagacttttc tcgccccaga ttttctatgt
    69541 ttataagtat cttttccttc ctcatccata tcaaaatcat gacaggtatg tttaataact
    69601 ccaacacgat accctttttt atttaattct tttattatgc cttcaatcaa cgtagtttta
    69661 cctactcctg aatatgagac aactgacaat atattataca taaaattgtg tcctttcata
    69721 aaactatcta ataacaacat ccactaattg attttctttt attgtctcat tctttttaat
    69781 cttaatcata caattacttc ttagagttgc agatagtcta gcttttgcat ctttcttatc
    69841 atttagctct acatacaatt taccttcact tgtatatagt tttccactta caaatctatc
    69901 tttatttgaa aatttattaa attctccttt aaatatagct tttatatgat gtgttccctt
    69961 cttatatgtt aaatactcaa taacaggaac tatcacatta tcaaatacca ctattgctgc
    70021 atgtggatta ccagaaagac caaataatag cttttctccc aatgtaccta cataacaagc
    70081 tccaccaggt ttaatgttta tcttccaaaa taattctgtt gcccctattt ttttgaatac
    70141 atctggaatt aaatctttat ctccaacaga aacaccacct gtggtaatta aaaaatctac
    70201 actctgtaac atattcttta cctttttttc taactccaat atatcatcag aacaattgca
    70261 tattgtatct gcttgtattc caagttttaa taatcttgaa tataaggtat atctattact
    70321 atcgtatatt ttaccattct ctaaagtctc atttatatct tgtagttctg tacctgtatt
    70381 caaaattcca acttttaaat catcataaac agttactttg gatattccaa gacttgctaa
    70441 tactccaata ccatcatatc ctatattctg atttttatta ataatcaatt gccccttttt
    70501 tatatcctca cccttaaata tgtaattatc gtattccttt aattttatat ttaaagttat
    70561 catatcacct ataactgtta catcttcttt ttttattaca caatttgcac cctgtggtat
    70621 ctttccacca gtcattatct tcactgcttc atttttattt aataccctat taaatacatc
    70681 tcctgcatac aaagtcccta taacctttaa atttctattt tcagaatttg cattatatgc
    70741 ataaccatca tagggtgacc tatcaaatgg aggactatca attgttgaat atatatcttc
    70801 tgcaactacc ttatttactg catctataag atttactatc ttgaccctgt ttattggtaa
    70861 aacatttttt tcaataattt ttatggcatc ttctaattct atcattttaa atactcccca
    70921 atatattttt tatttaagat atttctatgt aatatattat agattataaa agttggaaat
    70981 acttttatct tatatttaat tataactaat agttatctat aagtgcagtt caaattttat
    71041 aaatctaata aaaaaactgt tcccagctag aaatttcctt ctatcttgaa acagcatcat
    71101 atgctttaga atattttatt ctaataaagt tccttttatt atttttacaa ttttatcact
    71161 ttcaatagtt tttatagatt ttattttgtt atccacaata cttattgtaa gtacctcttt
    71221 ttttaatatt ttgttatctt ctttcaatct ataactctta actactttac tgccttcatg
    71281 attatatgct gttaaatcat aattcgcatc tcctacaatt tcatttattc tggaatcttc
    71341 aacaccaaca tactctccca ggtcaatatg tctaaaattt tctagtgata cagccgattt
    71401 ttcatggtca cttttttcaa tcactatact agattcagca tcaatgactg atataccttg
    71461 tgatgagaaa gaattggttt caacattgac aactttattt tcgttaatat caacatataa
    71521 agcacttcca tctagttcat catttccagt atattttgga taaactaatg tcatataact
    71581 tgatgtattt atatcttgca ttgtatttga ttcttttaaa actttaggtt ttatataata
    71641 aaatgtatta taaggtatac ccatattctt tataatgtac ttataatctt tattcataat
    71701 ttcattaaca tcattttgaa cttttgtcat agttttttgc tgtgttgctt tacttgctac
    71761 ttgatttttc tctttagtat tttctttatg acatccaaca agtataatac ctaaaatagt
    71821 tacacaaact aatgaccttt ttaacttcat aaatcaatcc tcctttctta aattttatag
    71881 tatttatcta tatctcaatt tacagttatc tcttttttca ataattatat ttattatatt
    71941 ataaaataat tatataatat ttatttgaat ttaataagat taagaagcta attgtaattt
    72001 ttctgtaata aaaaacactt taaagttgaa ttttgaaaat tcaatttacg atatttttat
    72061 tgtaagtacc ttacaacatt ataccaaatt attcctaaac aatcaaaaac tccatgaata
    72121 tttaaaggtt ttctatttta aaaatcacct ttgactatta atttatcaaa tatgttataa
    72181 tagttttaga taatttaagg aggattaaat aatggatttt aaacaacttg aagtatttgt
    72241 tgctgttgca aaacatcaga gtttttcaaa ggctgcaaga gaactttttt taactcaacc
    72301 tactgtaagc gcacacatac agaatttaga gagagagcta gaaactgttc ttataaatag
    72361 aagcaacaaa gttattacac tcacaaaatc tggtgaaatt ctttatgaac atgctatata
    72421 tatattgaat aattgcaaac gtgcaattta tgatataaaa gaatattcag gaaaaataga
    72481 aggtattata gatatagcat gtagttccat acctgaaact tatattttac ctgattttat
    72541 gaagagtttt tctatgagtt atccagatgt aaaattttcc ataagccatt atgattctca
    72601 atatgctata tcagaaattt taaatgaacg aataagcttt ggattagttg gttctaaaat
    72661 caataatcct caaatagagt acctagactt attagatgat gaacttgttc ttatcactcc
    72721 ttcagatttt aaaattgata ataaaaataa ttgtatcgat ataggagaat tggcttatct
    72781 aaattttatt atgagaaaag aagggtcagg tacaagaaac ttaatactaa acacacttag
    72841 taaaaataac tttccagtaa gtaaattaaa tgtaatcgct catgtagaat caaatgaggc
    72901 tattaaggaa atggttagat taggtcttgg agtgtccttt atatcgtaca tttcagctat
    72961 cgattatctc aatgcaggta aaataaaatg ttataaaata aaggatgttg attttactag
    73021 aaagtttttc ttcatttact ctaaaaagaa aactttttcc ccattagaag ataaatttct
    73081 aaacagatta tgtgagtatt tcgaaattat aatataataa ctttaataaa gtagtgctta
    73141 attttgagtt tataactaat taataagcta attataacta tcacataaat gcttataaat
    73201 tattatgaac catctttctt ttttatttaa tttagtcaaa aaatccctcg ttcaaatgaa
    73261 cttgggattt ttttatttat actactccaa tagcagccaa tactatacaa ataatcataa
    73321 ctgaaattaa tatcgtagtt gtctttacct tttttcctaa taaccaatac ataataccga
    73381 atatcatagc tggaagcata catggcatta tttggtctaa gtaagtttga ataggctctg
    73441 cccattctcc actacctaca ttaacactaa gttttaacat tacattagat gcagtcatac
    73501 ctccaattac cataagccct aacattgaag ctgcttccat aacttttgaa actatacttg
    73561 aaccactcac atctttaaag aattgagttc catacttaaa tcctttatct agacatatat
    73621 agcgtataat aaaatgagga acatttatta ttaaaaggaa taaaatcggt cccattatgt
    73681 taccctgaga agctaaagct ataccaaccc cagctgctat aagctttaat gttccccaga
    73741 aaaatgaatc tcctatccca gaaactggac ccattaaact tgatttaaca gcatttatac
    73801 tattagcatc aaattcttta ttctttgcat tttcttcttc catagctcca gatataccta
    73861 ccaaaagagt acttatatga ggagtaactg acataaattc taaatgtctt tttaaagcct
    73921 tagatttctt ttctttatca tctttatata atctacggat tattggtgcc atcatgtatg
    73981 catatgctat attttgcata cgttcgtagt tccaagatga atctaatgtc aatgaccttc
    74041 taaaacattt ccaaaagtct ttcctcgtta ttttactatc tggtgaaatt gtctctaact
    74101 tcttagaact cgttgtcatc tactattacc tccttctctc ctacaaagtt aacatatatt
    74161 aaagctattg ctaatccaaa caatgcaact cctgttactg ttatgtttag gtaagcaacc
    74221 aatataaatc ctaataataa aaatgctact gttttcttat taagcatcat tctagctaac
    74281 atggcaaaac ctattgctgg aatcacgcct gctgcaacat ccattccatg agttacatat
    74341 gatggtataa atgaaagtat tttttctatt actggaacac ctaatgaaaa tcctatacct
    74401 actattattg ctaatattaa tttatttcct ataccaaata ataattgcac acgttctacg
    74461 ccttcaatat tcccctcttc tgcataacgg tcagcaatat gagctcccca tgtaagtact
    74521 actgtcatta agaagttatc tataagcaat actagagttg ctataggaac tgcaagtaca
    74581 actgctgctg atacatcttg acccgttaat attacaaacg ctgtggcaat aatagaacca
    74641 gacacaaaat caggaggatt acttgcccca acaggaaatg cacccataaa tactaattct
    74701 aatgttgaac ctattactat acctgtatga agattaccta gtgctaaacc aacaagtggt
    74761 gcaacaacta taggtctatt atgcattgta gaacctacaa aactatgcat atatgcaaag
    74821 aatgcaatta gagttatcaa aataacttgt gtaaattcca ttttcatccc cctaaaatta
    74881 tattaatttc attacatttg tagcatcatc atcaggaact aattgtacaa atacatttac
    74941 gccttctgaa tctaactctt taatcatctt tatatcatct tttgatacat gcactgcttt
    75001 agatatattt tcacgattat catctgattt tgttccacca aggtttatag atttaatagc
    75061 ttttacctct ttagcaagtc tataaacatc ttctacagat tcacaaagta tcaataagtt
    75121 gtatttatca gtaacacctg agttaagtgc ttttatagaa tctgctatgc ttttcattac
    75181 taacttaaca ttagatggtt tagccatttt aagtcctgcc attctcaatt catcttttaa
    75241 taagtcatca cttgctatca atatacagtc tgtgcttaaa aactttgtcc atgtaaatgc
    75301 tacttgtcca tgtataagtc tgtggtctac tcttactaat ttaatcatta taattcctcc
    75361 ctaatttttt tgatttcatc catgaatgtt tttacgtgtt ctacactaac atccccaaaa
    75421 tctttgtaat tatctttaaa gtaacttcct attattgcca tatctcctaa tttaagctgt
    75481 ttttttgcat tttctaatgt aactccagca gctataacca atgggaagtc tcctaaattt
    75541 ttacgaaaca gttctatttt ttccattgat gtttcttgac ctgtagcatt ttctgtaact
    75601 gctatagcat cacatcttgt cattcctatt tttaaatctt cttcaacatc attttctgat
    75661 aatactggct gatatttaaa acgtacgcct ccaattaaat acgctgggca ttttgaacgt
    75721 tgtaatttaa aaaactcttc taaagtagct tcatctctag gtttaacatg acctactaca
    75781 gaatcaacct gtatataact agcattatat tttgttgcca gttcaaatcc catagtatca
    75841 acatttaaac agtttactcc atatggtatt gataaattag cttttgatac atattcaagt
    75901 attctttcta aatcataata attaccatag taattttcta acattattcc atctacacca
    75961 ttttcttcaa agattgtaat ctctttttta gctctttcaa agatatcttc tggagtatct
    76021 ccttttaagt gtatcattgc tataattggt tttttacttt taaatactga taatattccc
    76081 ttttttttca taaactaaaa tacctcttct tcttctttaa tttgtaatgt cacgctttgt
    76141 attcctcttc tagattcttc cactaattgg tctatatctt caacggttaa ttcatcagaa
    76201 aaccttgtaa gaagttctaa taccatattc aaattcattc cagaaaataa cctaacatta
    76261 tgtagattag ttaatcttaa cattgctgta catacacttg ctccatataa gtcagataaa
    76321 attatatatt cacattcttt atcttctgat attgtttttt ctaattctaa agcaaaatct
    76381 gaagcacttt gtccagggta caaaccatac gaactgacac aatctgctaa atctcctact
    76441 atcattttta ctgcatttaa aagccctttt gaaaagtctc catgagatgc taatattatc
    76501 tttttcatac tcatcctcct actaaattat agattatatt tctgctgata ctgcatattt
    76561 gtacttatca cttcttataa attgctcatt gtaatataag acatcattat tttctgtata
    76621 caccgattgt cttactttca atagtggcat attacttgca catttaagat ggtcagaata
    76681 ttctttgttt gaatgtttta cttcaaatat attttctact cgtttaggta atgtgcttat
    76741 attattagat ataacctcca ttaaagattt atcttctaat tcttctttta ataaaaacat
    76801 gtaatctatt ctaaaatagt caacttcaaa aataacaggt acttcatcta ttaatctaag
    76861 tcttttaata catattacct tctcttctag ttcaatactt aaagcttcag ctacttgctt
    76921 atctgctttt tttattaaaa cagagattat tttggtactt ggttttgcat taatacgatg
    76981 acaagaatta gtaaaactat ttcctgcaca tgttgcttct attctctctg gtattgttac
    77041 ataagtccct ttaccacgtt ttttacataa tatatcttct tttacaagtt cattaagtgc
    77101 atttctcaca gttatacgac ttatattaaa ttctttacat aagtctcctt cagatggaag
    77161 tttatctcct ggtttatata tgccagatgc tatttgtttt tttatgttgt catatagttg
    77221 ttgatataaa ggaacaaagg aatttacttc taaatccatt tctttttttt gtctcttttc
    77281 atccattttg ctcacctccc tatgtaacat ttttataaca ttatataaca agttctttat
    77341 tacatattat aacaagatga aatcgttgtc aatttttttt aaaataattt ttaatttgac
    77401 agtattttct tttattttct ctagtgtgac aaattgctat ttttacataa aaataagact
    77461 gttgtacaac agattaaatt gtttatacaa cagcctttat tcacttatta catttatatt
    77521 tttacttata aatttatatt aaaaaatgcc ttatatccct tatacatttc atatatgtct
    77581 actttagaga caatctcatg tggtgcatgc atatttaaaa ctccaacacc acagtctata
    77641 acctctgcac cttgatttgc caatatatgg gctatagttc cgcctcctcc ttggtctact
    77701 tttccaagtt cagcagtttg ccatactaca tttcctttat taaaaattct ctttacttct
    77761 gacataaatt cagcattagc atcattacat ccactctttc ctcttgaacc tgtatactta
    77821 gttaaaacta ctccatttcc catatatgcc gaatttctct tttcatatgc ttctccaaaa
    77881 tttgggtcat atccagcaga aacatcagct gataatacct tactatttgc taaagctctt
    77941 cttactttaa tatcacaata atctccttct aaagcaatca gttcagccac agtattttca
    78001 aaaaactttg agtgcatacc tgtattccct ttagaaccaa tttcttcttt atctacacat
    78061 aatgcaactg aagtaaattc tggttgttca gtttctagta tagcttttac agcagcatat
    78121 gaacataccc tatcatcatg cccatatccc aacaccatag acctatcaag ccctaaatct
    78181 ctagccttac cagctggaac tacttcaatt tcagcactta ataaatcttc ttcaactata
    78241 ttgtatttct catttaaaat ctttaatata tttgctgtta taggttcttt ttcttcatct
    78301 tctaaaggca tattcccaat taatatattt aaagcttctc cactaattcc ttctgatagt
    78361 ttcttttgca tttggtctcc agccaaatga atcaataaat cagttacaca aaatactggg
    78421 tcactatctt cttccccaat agatatgtcc acttttgttc catcatttaa tataactata
    78481 ccatgtaaag caagtggaat agccgtccat tggtattttt ttattccacc ataataatga
    78541 gtcttaaaaa aacctagatt actctcttga tacagtggat ttggttttaa atctaatctt
    78601 ggcgaatcta tatggcttcc tataatcttc ataccttttt ctatataatt tttacctatt
    78661 acaaaaagtg ctactgcctt ctctttattt ataacataaa ttttatcacc acaacttatg
    78721 cttccttttt ccatagcttc atctattgaa atatagttat tttctttagc caattttata
    78781 atttctttta ctgaaagtct ttcagtcttt gacttactta aaaactccat ataattgcta
    78841 gaatattgca taatttgttc aatagtattt tcttttcttt caaaatccca agcattctta
    78901 aattcgtact ttaagttcat attttcctcc tactaagttc taatttttat tgtttttcat
    78961 gtattgctca tattcttctg gtgaaactac tttgacacta tctagtcttg atctaaagtg
    79021 ggctctaaag ttttctaaat attctttcct taaaatttct ctttctttga tttcatcagc
    79081 agaaaggatt ccctctttat ttttctttgc taattcatta atcctatcta atttcttctt
    79141 gtcaaacatt ttttcctcct aaaaaacctc tatttattta ataaaataat ttattgcttt
    79201 ttatatctaa cttgttttta tattcacaaa aaaactcatc tatattatca cttattatag
    79261 atattccctt ttctagttct tccatgcttg gacgagctat atttatccta aagaatctat
    79321 catccactag attgtcaaaa aaataagtcc ctggaagcat tgacactcct ttgtttaaca
    79381 taaaatttgt aaatgcttga gatgtatatc ctcttggtaa ttctaaaaaa aagtttattc
    79441 ctccacaaga ttttctaact tttaatttac cactcaattt gtcattaatc aagttttttg
    79501 ccaatttata cttatcagta tatatcttct ctatatgact caaataattc ttccaatcaa
    79561 aattattcat atagtaatac attgattttt gaattaaacc tggtgttgat atatctgatg
    79621 aatatttagc ccataaaact ttttttaaca actcatttgg catttctaca attcctatcc
    79681 taagacctgg cataagtatt tttgaaaagc tttttatata tactactcta tttttatcat
    79741 cataacttct aattgttcta ttgtcctttg attcaaaaac aaaatcactt ataaaatcat
    79801 cttctattat ataaaaatca tgttcttctg ccaattctat caatttcttt tttttatatg
    79861 ttgagtaaga tattccagtt ggattttgaa aattaggcat aatgtatata agtcttggtt
    79921 ttattttttc caactttaat tttaaaatac ctatatcaat tccatcatct aacataggta
    79981 tagatattat ttttgcacct ctacttttaa acacttcaat tgctccattg taagaaggtt
    80041 cttccataaa aacaacatct gaataactta taagtccttt acaaactata tctatacctt
    80101 gttgagcacc tgatattatt tgaatatttt ccttagtagt ttttatattt ccatcactta
    80161 gatactctac cattttttct cttaattctt cagagccaag accttcatca tattcaaata
    80221 ttgaactacc atctttagat aatgccatat ttacagcatt tttaaatgaa tcgataggaa
    80281 acatatcatt agatggattc ccattgtcaa aatgaattaa atcttctctt tgcacaataa
    80341 tcttattttt cctgttatct ataccgcttt tataagaatt taaacttgat acaaaagtac
    80401 cactccctat aattttatat acatatcctt cattttctaa aagctcgtat accttaacta
    80461 tagtagtgtt atttacttgt aaaaaatttg atagttttct tattggaggc aatttttcat
    80521 gttctactaa ctctttattt attatcaatt cttttatatg attaaatatc tgtatatatt
    80581 tagttgattt tttctcattt aaatctattc tgtatttttc caaatttatc accgctcatt
    80641 gtatttatat tttattgatg cttttatgta tatttgatat tatttagtat gtatatataa
    80701 taccaaaatt tttataaaaa ggggaaatat ttatgtttta cgactatcac atgcactcaa
    80761 gtttctctac agacggaaaa tctacaatgg aagaaatggt aaaaaaatct attgaactcg
    80821 gtttggagga aatttgtttc acagaccatg ttgactatga tgtatatgct gatgattctt
    80881 tttcaattgt ctacgaagat tattttaaga gtttagagac tcttcaaagc aaatataaag
    80941 ataaaatatc aataaaaaaa ggaattgaat ttggagttca aactcaatta atggatacat
    81001 acaaaaagga agcacatcaa tatccattgg actttataat atgttctatc catgctatag
    81061 atacaatgga cttatacctt ggtaattatt ttaaagataa aactcaacat gaagtttatg
    81121 aaaattacta tctttattta tataatattg ttaagaatta taaagattac tctgtcttag
    81181 gtcatttaga tttgattaaa aggtatgcac cttatgacac tattttagat gatagactat
    81241 tttctgatat aattgaagaa actcttaaac aagctattta tgatggcaaa ggtatcgaga
    81301 taaatacctc ttgttataga tataatcttc cagacctaac cccatctaag tatatacttc
    81361 aaatgtacaa agacttggga ggggaaatca taaccacagg ttcagattca catcatataa
    81421 gtcaagttgc atgtgagttt gactacatat attcattact aaaaaatatg ggatttaaat
    81481 atgtatctaa atttaataaa ttgaaaccag aatttataaa attataaatt tttacacctc
    81541 cttgtgaata tatttaaaag ataaggaggt gatttttttg aaagtacctt atttagtact
    81601 atcaacagtt attttattta tatccaattt tattgtaaga atatttggtt ttttatataa
    81661 aatattctta tcaagagcac taggtgaaac aggtttaggt atctatcata tgatttttaa
    81721 ctttttgatg atttgcttag ctgttacaac aacaggtata cctactgcac ttagttgtct
    81781 agttgcaaag agaaaagctt taaacgatag acacaatact aatgctttat ttatatcaac
    81841 tttatatata tcatttttcg tagcactaat tatatctata gttgcatctt tcaatagttc
    81901 ttttttatca cttaaatttt taaaagatgc taaattaaat ctatttatac tagctgtttg
    81961 tcctgctata gttataataa ctctttcaaa cgtactaaga ggatattact atggcataaa
    82021 gaatgtaaaa atccctgcta ttggacaaat cattgaacaa attggtaaaa tattatttgt
    82081 ttttttactt gttatgtaca taaataataa atctatgaat tgttatatag ctctattagg
    82141 tatatcgatt ggtgagttga gtaatattat atttatgctt atttgcttat ggcgagattc
    82201 atcatttgac aataggtata ttataagcat aaaagatttt tataattctt ctatggaaac
    82261 attaaaaatg tctattccca taacttgtaa tagaatgtca aacatacttt tgcaatctat
    82321 aagttctatg atgataccat caagattagc attatcagga atgacttatc aacaatcttt
    82381 aagtatgtat ggtattgtca gtgggatggt aatgcctttt atatttttac cttttactgt
    82441 tggttcagcg cttattgtaa atttaatacc aactatttca caagaaatgg ctctaagaaa
    82501 gcgtaagagt gtaattaaaa aaatcaaata ctccatactt cttactttat ttgtaggcat
    82561 actatcatca atattttttt atttctttgg aaaagattta tgtatattag tatttaaaaa
    82621 tacacttgca ggagaatatc taaaagctat gtttttagtt ccactattta tgtccctaaa
    82681 tcaaactcta tctggcattt tacattcaat aagaaaagaa ttagcttcaa gtataaatac
    82741 tattactggc atgttaattc aactaatagc tttatatgta tttcttccaa ttccaggact
    82801 taatatttat gcatacatat acacaatgac gatagtatca atctttactt gtctattaca
    82861 tactatagtg ctttttaaat cactaaaatc tatacgctag ttataaaaac gtgaaggcta
    82921 ccaatgtaaa ttatacactt tggtagcctt ccttagatga tttttattta taatttaaac
    82981 tgtctatata tctacctatc tatctgtggc aacttggctt tccacttcca tgattaggtc
    83041 cacattgcga aaaatcacat actttagaga aagtatcaac aagtcttggt tggattggat
    83101 ttggtgatgc taaaactact atttgttcac ttattaatgt gctaactgtt tcttgtaagc
    83161 aaagtactaa atctatatca tttggtataa ttaattcata gaaaggttca caacattcgt
    83221 tgcattctaa ttttaatata gattttccta tacaatctac agttaagtta gtttgatatt
    83281 cttgtcttaa agtaacagct cttccgtcag ctggcaagca tattggtgct gaaagtgtgt
    83341 taaaagtaaa ttctacataa tcaactcttc tcttacatcc acctcctgct ctaactgctg
    83401 ggaaagctaa tgcaacgaac tctgtacatc cacatctacc tcttagctct agtactaaat
    83461 tacgaactgc tacagttaat cctctttgtt tataagctac tgatttgcct tttccttgtc
    83521 tacagcactc agagtttctt tctccacaaa ctgcaaattc aaaagttgtt tcacagtttc
    83581 ttcctactat atctcctaat gggtctaaat cgaaatcttc taaagttgtc attcctgtgt
    83641 ctataacgat tccatcatta cttaagcata ttttttctat agttacactt gcttgacctg
    83701 cacttggaac tgaaccaaat acttctacta cttctgtttc aaaagttaat ggctctccat
    83761 ttggtcctgt tgcatctgta aatgtttgga attgcatagt atcaaatatt ctattagcct
    83821 ctattgcaaa tggcactgca tttgatacag agaagtttct tggagatata ggattacagc
    83881 attctggtcc acatttgttt tcaaaacatt catctgaatc catttcacat ggttcacaat
    83941 cacatttaca attgtcatgg catccacaat tgtcatcaca tggattaggt ttgcatggat
    84001 tgcatgggtt aggtttacat ggattacatg ggttgcatgg attgcaatca tcatggcact
    84061 cacacttatt atcagtatta catccattat gatggtgtga ttttttaaaa cctctcatgt
    84121 cttctgaatt tatttcatct gtatacactt cttcttcatt cattgtagac attggctgat
    84181 tcatcattct tcttttattt tttttataat cttgcatttt tcattgccct ccctgtaagc
    84241 gattcttatt aagtctttgg ctttttgaag cctaattaat actatgcttt tataatctta
    84301 attgttactt gctttttgaa tttatcaata attaggcgta tttagtagaa aaatgcatat
    84361 taataaaaaa agtactacta tttgaaatag tagcactttt tttgtcccga aacaccggtg
    84421 gctaagtaaa ttcacaccct aggcactccc aggtgggtga cttgccagta acctttaacg
    84481 tccaaactaa ctaggcatgt tataatttac cagacacggt ctcccgtatt tcatttgtag
    84541 gttgaattta aaaaagcctt atcgtatgtt tcaggatttc atattattat tatataataa
    84601 attttgaatt ttttaaagtg gttttatata gtaattgaga taatcttatt ctatcattac
    84661 tttttcttcc ttttttcttt atgtatttag ttaattatcg ttttttctta gctctagttt
    84721 tagtattttt ttcattataa ttgtattcgg attcttgctc attaatgttg tatagttcct
    84781 catcgtatac tatatcttca tcattttcta tattttcata gcctgtgttc ttatcttcta
    84841 ctaattcatc ttcattatcc tccatttcac cttcatctgt taactcatca tcatccaatt
    84901 ctttgtcttc acaatctaat tcttcgtctt cataatttaa ctcatcatta ttgtcttcat
    84961 catcattatt actatcatca cctcttaaaa gacttcctaa caatttgaga ttattcatac
    85021 taccaagatt gtttatattt gccaaagagc tcaaactaaa tccaccttca ccaaagtcat
    85081 ctggtaaaaa taattctgca aataaatcta tcttcttttt cattcctata gctttttcaa
    85141 ctgaattttt tcttttttct gggaatacat ctataatttc ctttatcatc atattctttt
    85201 tatcatcact atccattttt ctgaaaaaat ccgtttcatc ttctgtatct accatatcta
    85261 tattatttaa tttttttatt cctctaacta aatctaaaac actttctatt tttattagta
    85321 tttttctttc atctcttgac atgtattttt tagttcttgt tattatttcc attcccattt
    85381 ctaaatcttc ttcacttaaa tccatgagcc ttaaacctct ttttatatca tttgcatcaa
    85441 accttaaatt gccatgtgta gactgtgaaa gtttatcatt tgcatttttt ttgttttttc
    85501 taactctatt atttcctttt gatttatttt tatcatcaaa tatagagata ttaccattac
    85561 tcaatgcaat cactccaatc aaaaatagta agttattcaa gacaattccc ccttatatta
    85621 tataattgcc ctatattaaa gtatgttttt catattttaa tgttccattt ttaaagctca
    85681 gtgcatatat attatctata agaattttta tttatattat atatcactca caattcacca
    85741 cttcaatatt atttctatat ataaatagta tgaatataat tcaatatata tttatagaaa
    85801 ggggtttttt tatgaaaaaa ttagatacag agtcattaga aaaaatggct aaacaaaaaa
    85861 atattgacaa ggataaaata gaaaagatgg ctgatagtta caaaggaaaa tcagaaaatg
    85921 aacttatgga ggaacttata aaaataggta agaatctaga tggcagagat gaagtagtat
    85981 caaaattcaa agcattttta gatgaaaatc aacgcaaaaa actggataat ataatggaaa
    86041 aaatctcaga tgcagaaaac caaagagata ctaaaccagc taaaactaag aaggctaaac
    86101 ctactaaagg taagtcaaat tcacctgcac caaaaaatac accaggtagt caacaaaaaa
    86161 aatctaaaag tttatttaag aaaactaaaa agtctaatcc tcatgaatag ataaaaataa
    86221 actatttaag caattagata attattttta gttaatctat tattaaattt gatattattg
    86281 acataaaaaa tcaggtagtg cctaaggcac tacctgattg attttaatag ttttattttt
    86341 ttctttctgt aacactttct ctatcaacaa ttctataagg taactctatt ttcttatttt
    86401 caattaattc cttatttatt aattttataa ccattcttat tgctactgct cccatatcgt
    86461 atagaggttg atatactgta gtaagtttag gtctatacat tctagctatc ttaacatcat
    86521 taaatcctgc aactgatatg tcttctggaa ccttatatcc agcatcacat atagcattta
    86581 tagcacctat ggcagcctca tcacctgtta caaaagccgc atgaggtatt attccatcat
    86641 ctaatagttc tttcatacta ttgtatccac tctcataatc agttccacca tacttaatca
    86701 aactcttgtc taattctata ttgttatttg aaagtgcttt ctcataacca gcaagtcttt
    86761 ccatttctaa aacagtatca tctttactag tcattataaa agctatcttt tcatgacctt
    86821 tctttataag atgctctgtc atatcaaaag tagcttcttc gttgctagta cttactgtat
    86881 atatatcata atttctagca gttttactta tatatgtcgc tggtattcca caattttgta
    86941 tatagttgat atgttcttgt tcaactatcc atgaaatcat aactatacct tccacttgtt
    87001 ttgctctcaa tagatttata ctcttaagtt cttgttcctt atcagagtat gtattcgcta
    87061 aaagaatatc atagtcatac attttagcaa cctcttctat cccatttaac acctcattaa
    87121 caaaagaatc tgaaacttct ggaactatta cccctattaa ttgactcttc tttgttacta
    87181 agcttctagc aagtggattt ggtatatatc cagtctcttt tataacctct aaaacttttt
    87241 gtttgacttc atcagttaca ggttttgaat catttataac tctagataca gtagatattg
    87301 acactcctgc ttgtttagca acatctttta tcgttatatt gcctttcatc ttcatcctcc
    87361 ttattttact aaatttatct aaaactgaac caaatttttg aaggttccta ttttaactac
    87421 atttatataa gtttttattt taccataaaa gcaagtacat ttaaatacat tatattttat
    87481 attataccct atattcaaca aatttaataa tattcattct taatttaaat catattaaaa
    87541 aataaaataa aaaaatgctg gtataaaata ccagcatttt tttattctaa tgatttaagt
    87601 attagcagcc gcaacctact tcaccatcaa agaatccacc acatgaacaa gttattagca
    87661 ataatattaa gaaaggtatc catgacatta tgtcaatgtc tctccagcaa tcttcaaaag
    87721 ctaagaatag gaagaatagt actattatcc accatcctcc tccaccaaag catccttctt
    87781 ctccaaaaaa tctatctaac ataacaaagc cctccttttt ttaataactg tgttcttatt
    87841 ctaagcatcc accacagcag caaagtatta ataaaactat taggaatggt atccatgcca
    87901 ttatgtcaaa ttctgcccag ctatctccaa aagctaagaa taggaagaat agtactatta
    87961 tccaccatcc tcctccacca aaacatcctt cttctccaaa gaatctatct aacattatac
    88021 gttacctcct gttgatattt tgttgagctt ttcgctccta acatatacta tgcatttggt
    88081 ttccattttg ctaatgtatt tttactattt ttttaaattt tttaatttga actaaaaaag
    88141 gtaaaaccat aacttgtata agttactggt tttacctatt taataatcaa tattttttag
    88201 caacaacaac catcatcgaa gttaaataat aacaatacta ttaatataac ccatatccat
    88261 atattgtcat cgcataataa gtcttctaaa atgtctgtgc ataatagtaa gaatactata
    88321 actattatta tcaacatttt attcccaaaa aatccttcgc atcctccaaa gaaacgttcc
    88381 atattgtctt ctaattttgt cataactaat cctccttgaa tcattaagag actaagtttt
    88441 gtctctttaa ttaaatatat gtagtaatag acagataagt tacttctaaa aagtttttat
    88501 tgattagttt atttatttct ttttcaccaa gtttattgta tctatcaact tcatccagat
    88561 aatctttttt ctcctctgat atagtataga ttaaactttc tattgatttt attttgtact
    88621 ccttatatct ttctatcaag aaggatgttt ttgaattttc tattcctttc tttacatgca
    88681 ttaaccttga tatgtaatct tctttttcat tagatacata actctccttt tcagctacat
    88741 cttcaaggat taaattggtc tccattacat aatcctctat acgttgcata tctaaagtta
    88801 ttgaattata ataaaaattt actgctataa gtaaaaacat tcctactgaa gttagtaata
    88861 gtcctacata tttcatagca tttcctccta agttatattt gttatcaata aagtttattc
    88921 atcaaataat actttattac tacatttata aaattttatt attttttata gattccatat
    88981 tgaaaaattc ttgatattat attaaactaa cattaaaatt aattaaaact gctttaggag
    89041 gcttaaaatg tttgagaaaa taaaagaaat aatagcagaa caattagggg tagacaattt
    89101 agatgagata actatggaag catctttaat ggacgactta gaagctgatt cattagatgc
    89161 tgttgaaatt ataatggccc ttgaagatga atttggaata gaaataccag acgaggaagc
    89221 tgaaaacttt aaatgtattg gtgatatatg taaatatatc gaagaaaaca aatagtatcg
    89281 ttttgtctac aaatataaat agtctgtgta aacttctaca taataaatta tattagagtt
    89341 cgcacagact agattacatt tatttacaat atataaaata cacgctctta tatttaattt
    89401 atatactaag tatataaatt acttttcttc ataaaacgta acattcttta ttattatatc
    89461 tatagttcca tcttgatttt gttttacagt atacttcatt gggtcttcaa aatcagttag
    89521 attccctttt atgtcaaaac cattatccgt ttttatactt ctttttttaa gctttttctc
    89581 aacccatttt ttatctatac taaaactttc gtcaagacct ttttcttcca tatgctcttt
    89641 aaagctatcc tttaactcat catctttaat tgaattatca acaaagtcat ttatatcaac
    89701 ttcatgtttt tccttcagtg tataatttaa tatacttctt acatcttcag cttgctttat
    89761 atcattacta agagcattgg ttatccaatt ttcagctgta tttttaaata ccttggtctt
    89821 gtacttatca tcatctattt ttttagcatt taaaaattct gttacaaact tagattctga
    89881 cccttctttt tcagcatcct tatctaaaag ccttagatga aactcatcat ttataccact
    89941 tagactaatc aaagctcctt gcttttgtct taaagtctct gggataccaa tttcatttga
    90001 aaccatttga atattaaatt tatcatccac aaattcaatt gaatgagtgt acaacttttt
    90061 ataatctaat ttcagtattg ctacactctt ttcatcttta atagtgtata agcaaattgc
    90121 caaatcacaa gattctagtg cagcattaat tttcataaca tcaaacaagt aagatgcaat
    90181 ttctttagaa tttttcaaaa aagtactttc atcatatatt atttgttcac aacagttttt
    90241 aattaaattg tcattataat ctttaaaaac accttttctt aaatcctcat cttttgcaat
    90301 tctttttata gctttctgga aaaaaccatc tacttcttga ttaactttgc cttcaaagtc
    90361 atttaatatt ggaacatcac tatttttgtc caaaacatgt attataaatt tatgtattat
    90421 catatatatc accccattat cttaatttcg atattaactc tttcatatcc tctggtatat
    90481 ctgctttaaa tttcaatttt tcttttgttc ttggttgttc aaactctagc ttatatgcat
    90541 gaagtgcttg cctatttatt agatcttcat ctacataacc atacaactca tcacctatta
    90601 ttccatgacc tatatgagcc atatgtactc ttatttgatg tgttctacca gtttctaagc
    90661 ttacttctaa tacagttgca ttttctagtc tttctattac tttatagtga gtaacagatg
    90721 actgtcctct ttcatctatt atcctcttta ttgaatcttc tgtaggtcta taaattggct
    90781 catctatagt tccctcattt tcttttataa ttccatcaac tactgttata tacttttttt
    90841 caactttatt ttcactcatt gctgtagaca aagtatgatg tgcataagca ttttttgcta
    90901 ctataactaa ccctgaagta ttcatatcta acctatttac aaatcttatt tttacatttt
    90961 ctttttgatt atctatatag taactaatac catttgctat tgttttatca tagtggcttt
    91021 ttgttgggtg aactaccata aacggaggtt tattcaccat aattatatca aaatcatcat
    91081 aaattatctg taaatttaaa tcttgaggtt caaaatttgc tttttcctcg tctattttta
    91141 cttctattaa atcaccacta tacaccttta aactcggttt tttatatact ccatttacta
    91201 aaacactctt ttccctcttc atttttgata atgacctaac tgagaaattt agcttgtcca
    91261 ataaaacttc ttttaaagtc atttcttctt catttgtata cgatataaga ttatatcttt
    91321 gattctcttt tttaaacaaa cttttaccta ctttctttta tatcttccta cttttttata
    91381 tcttcaaaat catgatatta tatattttac actatttttg tttaatagat aataattatt
    91441 tttaactata aaaacctgta agaataacaa atagttattc ttacaggtta aattcataat
    91501 taaatattta atttgtaaat attataatct aaaactatct cttaactatt aaagtagttc
    91561 ccattcctcc acctatacaa agtgtagcaa gaccagtttt agcatctctt ctcttcattt
    91621 catataaaag tgtagtaagt attcttgctc ctgagcatcc tattggatgt cctatagcta
    91681 ttgctccacc attaacatta actttgttca tatctatatt taagtctctt attacagcta
    91741 cagattgggc agcaaatgcc tcattagctt caactaaatc tatatcttca atagtcatat
    91801 tagcagcttc taaagctttt ttagttgctg gaactggtcc atatcccatt attttagggt
    91861 caacaccagc tgttccataa gaaactatag ttgcaagagg ctctattcct agttcttcag
    91921 ctttttcttt agccattact actagcatag cagcaccatc atttattcct gatgcattac
    91981 cagcagtaac tgttccatct tttttaaatg caggtcttaa cttagcaagt ttctccattg
    92041 tagtgccagg cttaatatat tcatctttat ctactacagt gtcacctttt cttcctttta
    92101 taacaacagg aactatttct tcatcaaatt ttccttcagc ttgagctttt tcagctttat
    92161 tttgacttgc aagagctaat tcatcttgtt cttctctagt tatattccat tgctctgcta
    92221 tgttttcagc agtaataccc atgtgatagt tattaaatat gtctgataat ccatctttta
    92281 tcattgaatc aacaaaagca gcatcaccca ttcttgcacc atatctcgca cttggtacta
    92341 aataaggaga catactcatg ttttcagctc caccaactaa cattatatca gcatcaccta
    92401 atgctataag ttgagatgcc attgaaacag atcttaatcc agaaccacaa actatattta
    92461 tagtcatagc tggtttttct actggtattc ctgctcctaa tgctatttgt cttgctatat
    92521 tttgtccaag acctgctgta agtactcccc ctaaaagaga ttcatctatc atatctggag
    92581 ttatgttagc tctttttata gcttctttag ctgctgttac ccctaactct accgctgaaa
    92641 ctgatttaaa tgctcctcca aaacttccta ctgctgttct agctgcactg gcaattacta
    92701 cttctctcat attcattacc cccattaaat tagatttttc atatatccga aattgttttt
    92761 taaacaattt tatttaaact tttaattccc aaattcttat ttattataat catagaatcc
    92821 tatcttagtt tttcttccta attgattagc tctaaccatt ttagctaaaa gtggatgagg
    92881 tctatattta gtatctccaa actcagtata taaaacgttc attatagcta aaacaacatc
    92941 taatccaatt aaatcaccta atgctagtgg tcccattgga tggtttgctc ctaatttcat
    93001 agcttcatct atttcttctt tacttgcaac accatctgca tatataccaa cagcttcatt
    93061 tatcataggt ataagtattc tatttactac aaatccagga gattcagata catctactgg
    93121 tactttattg atactcttag ataattcaaa tactgtatca aaagtaactt ttgatgttaa
    93181 ctgaccactt ataacttcaa ctaatttcat cataggaact ggattaaaga aatgcattcc
    93241 tataacttta tctggtctct tagtagaaga agctatttct gttatagata atgatgaagt
    93301 atttgttgcc aagatagtat cttctttaca taattcatct agtaacttga aaacatcttt
    93361 ctttatattc atgtcttcta cagatgcttc tattattaaa tccatatctt ttaaatcttc
    93421 atagttagta gttgaactaa catgacttaa tatttctgct tttgtagctt catccatttt
    93481 tcccttagta actaacttag ttaaattttt atctaataaa gctaaacatt tatctatagc
    93541 accttgagtt ctactcttta aacatacatc atgtccacaa cttgcaaaag tttgtacaat
    93601 accacttccc atagttccac tacctattac agctaatttc atagttataa cctcctgtta
    93661 ttttagtatt tatattctgt taactagatt aaaatactat ttaatctagt taacagcaaa
    93721 tatattttta attagatttt taccctttta taaagttagc ttctctcttt tcaacgaaag
    93781 ctgacattcc ttctttttgg tctttagttg aaaaacaaag accaaataaa ttagattcta
    93841 tatctattcc agtatttata tcagtttgag caccaagttg tattgcttct ttagagtatc
    93901 taactgcaag ctgagcattt ttagctatta tcttagctaa tttctcaact tcttctatta
    93961 aaatgtctgg ctcaacgact ctatttacta gccctatttt ttcagcttca tcagctttta
    94021 taacttgacc tgtaaagatt aattcttttg cttttgccat tccaaccaat cttgtaagcc
    94081 tttgagttcc tccatatcct ggagttattc caagagttac ttctggctga ccaaatttag
    94141 ctttagcaga tgcaattctt atatcacatg ccattgcaag ttcacatcct ccacctaaag
    94201 caaatccgtt tacagcagct atcactactt ttttactatt ttctatttct ccaaaagctt
    94261 ttgctcctaa gatactaaaa tctttagcag ctacagcatc taaatctttc atgtatgcaa
    94321 tatctgctcc agctacaaat gcctttcctt cccctgtcaa tattacaaca tcaatagttt
    94381 catcattatt aatatctaca aatacttcat aaagttcttc taaagtcttt gaatttattg
    94441 cattaagggc tttaggtcta ttcattttca ctgtacatat atttccatct acttcaacag
    94501 ctacattctc ataaacttta acatcacttg tactcataat accctcccca agaaattagt
    94561 tagctaaaac ttcacctttt tcttttgcaa cacttaactg actgataagt tctggaagca
    94621 ctttatgaac atctccaact ataccaacat cagcatattt aaatattgga gcttctggat
    94681 ttttatttat agcaactata aactcagcat cttccatacc agctatatgt tgtattgctc
    94741 cagatatacc acatgctata taaaggtctg gtcttacagt tttaccagtt tgaccaactt
    94801 gtcttgcttt atctaaccaa cctgcatcta tagtggcacg agagccagaa acttctccac
    94861 ctataatttc agctaattca taaagtatgt ctaagttttc ttttccaccc attccacgtc
    94921 cagcagaaac taatatctta gcatcttcta ttttaacttg ttttttagct tcttttatta
    94981 cttgtacaac ttgaactaat ttatcagcat cattaaattc taccttgaaa cggttaatta
    95041 cagcttcttt agtttcatca ggttcatttt tcttcataac ccctggtcta actgtagaca
    95101 tttgaggtct gaaatcttta caaactattg ttgccattat atttccacca aaggcaggtc
    95161 ttgtcattaa taataatttt gtatcttcag ctactgcaag acctgtacag tcagcagtaa
    95221 gacctgtatg tattctagca gaaactctag gcgctaaatc tctacctatt gaagttgcac
    95281 caaataatac aactataggg tcagctgctt ttattgcttc ataagctgct tttgtatatg
    95341 gttcagttgt atacactgct aaagcttcat catctactac tattacctca tctgcaccat
    95401 agtgtgctaa tgtatctatt aaaccttcta ccttactacc taaaagtaat gcagaaactt
    95461 ttgtatcata atcttttgct atttctgtag cctttcctag taattctaaa gaaacagttt
    95521 gaattacatt ttctctttgt tctattacta ctaaaacgtt acccattctt tatcccccct
    95581 taaattatat gatatacttc tcttttaatt tatctatgat aattccagct gatgttttcg
    95641 catcttcatt gtatattgta ccagctggtt taactgattt tgtaaatgat ttaaatacac
    95701 tagttggaga acctttaaga cctaaattag aagggtcaac ttctatatct tttacagtcc
    95761 atgtttctac tacatcattt tcgaaagcat catatattct tccaactttc atgtatcttg
    95821 gtgtgttcat atctttaaga gttgttataa ggcatggcat tttaactttt aagtcatggc
    95881 aacaatcttc aaattgtctt tttactaata catattcacc ttcagttttt atttcttcag
    95941 catatgttat tgatggaaga tttaaatgtt cagctatttg aggtccaact tgtgcagtat
    96001 ctccatctat cgcctgtctt ccagctatta taatatcaaa atctatattt tttaatgctc
    96061 ctgctaatgc tgatgaagtt gcccaagtat cagcacctgc aaatgctcta tctgttaata
    96121 atatacctct atctgcaccc attgctaaag cttcttttaa agccatatct gcttgaggag
    96181 gtcccattgt tataacagtt acatgagcac ccatttcttc ttttaatttt atagcttctt
    96241 ctaaacctgc tttatcatca gggtttatta tacttggtac tccatctcta attaaagtac
    96301 ctgtattagg atctagttta acttctgttg tatctggaac ttgttttata caaacgacta
    96361 tattcataaa tccttcctcc atatctattt taatatcgac ctattgtgta tattttaata
    96421 tttatctatt ttaataattt tcctgaaata accattcttt gaacttcact agttccttca
    96481 tatatttcag ttatcttagc atctctcatc attctttcta ctggatagtc acgagtgtat
    96541 ccatatcctc catgaagttg tacagctttt gtagtaactt ccatagctgt ttcagctgca
    96601 aataattttg ccattgctgc ttctactcca taaggttttc ctaagtcttt atttatagct
    96661 gcttgatata caaggtgtct agccgcttgt accttaactt ccatatcagc taattggaat
    96721 tgtgtatttt ggaattttga taatggtcta ccaaattgta ctctttcttt tacatattta
    96781 acagtttcat caagagcacc ttgtgctaaa cctaaagctt gtgcagctat accaattcta
    96841 ccaccatcaa gagtagacat tgctatctta aatccttgac cttcttttcc aagtaaattt
    96901 tctttaggta ttctgcaatc ctcaaatatt aattcactcg tagctgaacc tcttataccc
    96961 attttctttt ctttaactcc aaagctaaac ccaggagttc ctttttcaac tataaatgct
    97021 gatattcctt tgttcccctt agatttatca gtcattgcca ttactacata tatgtcacca
    97081 gctattgcgt ttgttataaa tatttttgag ccattaagta tgtattcatc cccgtctaaa
    97141 acagcagttg tttgttggcc agacgcatct gtaccagcat taggctcagt aagaccaaat
    97201 gctcctaatt tttctccact tgctagtggt cttaagaatt tttgtttttg ttcttcatta
    97261 ccatattgat atataggcca tgagccaaga gatgtatgag ctgataatat aactcctgta
    97321 gtaccacaaa ctctagacaa ttcttcaact gccattatat atcctacagt gtctccacct
    97381 tctccaccat attcttttgg atatggtata cccatcattc ctgcttttgc cattttttcc
    97441 actgtttcat aaggaaatct ttcttcttca tcaagttctg ttgctaaagg tttaacttca
    97501 ttttcagcga agcttacata tagctcttta agcatctgat attttttaga atttaaatcc
    97561 ataatagcct cctcagtatt ttataaatat tttattttca tttattaatt aaaatctcgg
    97621 caacatctat aataaggtat ttgaatatac gtcgagaaat ttataacctt aatcaaacta
    97681 cgctctatat ttatatgtga ttatactaac caattatgac attaatttaa tcataaattg
    97741 ttaatttaaa atcaactata cttaaaaaaa atctcgcttt ttggaatttt gtgaaatttc
    97801 aagttagaat aagatttctt tcacactggt tacagataaa ataatttgtt tacgatattt
    97861 atattagggt atattaaaca ttaaatattg tctgtacatc tgttttttag ccatgtacag
    97921 aagattttta tcgtataaaa attattttaa tagagctaaa taatcgtatt tttatctgac
    97981 tattttttat ttttaaatat cttacatcta taatagtatc actcaaagga taatttttca
    98041 ataatttcta attatttttt taacaatatt tattacattt tttccacttt tttgtcattt
    98101 ttttattgtt tcgaaaaaaa atcagaattt tttaacttta ttttggatta ttagtttaaa
    98161 ttagattttc ctcttagatt tataaatatt tacacaaaaa aactacctct gtatagattg
    98221 tatacagagg tagtttttta ctaattccta ctatagttta ctatattttt ttaccaattc
    98281 ttacaaacat ctacccattt ttcacactta gaaaacttct ccatttcttc acatgtaagt
    98341 tctattgcag agttactact gccacatgca ggaaatactg tatcaaatct tttcattgaa
    98401 tcatctaaat agacttttat agaatttttt aaaccaaatg gacagactcc accaatagca
    98461 tgtccagtaa actccaagac ttcttctggt gtcaacattt tagccttaca attaaattca
    98521 gccttatact tcttattatc aatcttagca tcaccagctg tcacaattaa aattgcatca
    98581 tctcctattt taaatgagag tgtcttagct attcttgctg gaataacccc agctgcttca
    98641 gctgcaagtt ccacagtagc actagattgt tcaaattcta atatactgtc ttccttgcca
    98701 aattgtttaa aatactctct tacattactt attgacatta aatatccctc ctagtctgaa
    98761 cttttataaa tttcatatta ttcttaatta tatctataat tgatttattt ttcaaattat
    98821 tccatgatat tagcattcca tgtatcatta ttttttccaa ctactagata aagtcctttt
    98881 gccattggaa tattgacaat atattctcct ttatactcta cactcatacc agattcatta
    98941 aagtacctat aatcaaaatt tagtcctttt ggaattacaa aatcaacata accttgatta
    99001 tttggtttta ttccatattt cagagttctt tcagttatag aatttttttc attaaataaa
    99061 tctacattta catatgcaga acacttattc attccctttt tattttccac acaattttca
    99121 aacttcattt tagcactgtt taatttataa ctatatttac tattattgct tattttaaat
    99181 cttacaatag taccaacatc tctacttctc ctgctatcaa aatcaaaaac aatttcctct
    99241 aaatgattta tttcataatc tttgacactt accatatctc tatatactaa tttatttata
    99301 gctatgaata gtgtaaaaat ccccaaaata aaaaatgaaa cttttataaa cttcttcatt
    99361 tttagtctcc ttatattttg tgctattgtg ctataatatt aatcaaaatt ttaataatta
    99421 aacttattct tttttgaaat cttaatagaa aaatacattg atattataaa aattattatt
    99481 gttaaaaatg tcatactata taaatttata tacttagtat tagctatatc agttacataa
    99541 tttgaaagat atactcctcc aactttacta taattaaaat gctttacttg atatacaatc
    99601 atactaatca tcttcaaaag acttccatat acaaagtaaa ttaaaatact tactataaaa
    99661 ccacaaactt tcatttttcc atatccaaac ttaaaaatta aaggcagtaa aatcgtcaaa
    99721 attgctaaaa atatatttac agaaaaaaat acatcattta aaaccatatt tctaaagaac
    99781 actagtgatg tcaatttact tagtaaggac acaattattg ttacagaaat tattaatcct
    99841 acacctaata aatattttga gtaaactata tcttctatac taactggcat actccttata
    99901 aaaatccttg aatcattttc ataatcacac tcaaacgtat ttataaatat caaataacta
    99961 acaacaatac ttactgtata atatgaaatt gaattaagga aaaaataaaa cacaaaaaat
   100021 agcaatatgt actttaaatt acggatacca tctgaactaa tccctattat taaatctttt
   100081 cttattaaat tgaacatcat ctcacctcat ttacataata atacattagt tcttctatag
   100141 taggattttt gaccttatgc ttatcaattt ctatcaatag gctttttact ttcaagctat
   100201 ctccaacttt aacaagtgcc tcattataat atttatactc tttcacacca actatgcttt
   100261 ctttaattgc ttctaactct gctttatttg ccctaattat cttatattct tgttctatta
   100321 attctttctt aacagtaaaa atctgatttc ctctatttat aaaaacaaga taatcagcaa
   100381 ttttaattaa attatcagta ttatgagtag aaattattac actactactt ccatttttca
   100441 caaaatcttg taagtactgc attatttcag ttcttacaat tgggtctatc ccagcagttg
   100501 gttcatccaa aatcaataac ttagcattgt gactaagtgc acttgataac ataagctttt
   100561 cagattgacc tttagataaa ttttcaatat gcatattttc ttctatatca aacttattaa
   100621 ggtattcatc aaacctttct ctatcaaaat tcttataaaa taaagaaact atttttctaa
   100681 attccttaac ctttaaatgc ggataaaatt gtaaagaatc atatacaaaa ccaatattgt
   100741 ttttaaagct tattggagaa ttttcaatag tttcattaca aagttcaatt tcaccttcat
   100801 ctgcatcaat aatccccatt ataattttta ttaaagtcgt ttttcccgaa ccatttggtc
   100861 ctataatccc aacaatatcc ccttccttta actctaagtt tatattattt aacttgaatt
   100921 ttcctaattt ttttgtgata ttccttatgc taaacacatc ttcacctctt atgcattttt
   100981 ttatacccac ctatacatat tttaacacat tcactacaaa ggtcatttat aaatttataa
   101041 attaagaatt ataaaactat gttataatac aaagtatgaa gaggtgaata tatgaaaaga
   101101 attataacta taaatacaaa ccaattaaat aagtcccttg agactaaaga actcttaact
   101161 agaaaactta tcaatgctgg ctttgaagtg tattcagata tttatccaga cacagagctt
   101221 attatatcaa ttggtggtga tggctctttt ttaagaacag ttagagattt cgattttcct
   101281 gagattccca taatgggaat aaatacaggt catctggggt tttttcctga tattttacca
   101341 gataagattg atagctttat tgaagcttat acaaaaaaag attatattat tcaagaaatg
   101401 tcacttctta atgcggaagt atatacaact actagtggtt ctaatatgct tgctgtaaat
   101461 gaggttgtta taagaggaga caagtctagg actatacacc taaatttaag ccttgataat
   101521 aaacatatac aaaattttag tggagatggt atgattattt ctacttccac aggttcaaca
   101581 gcctataact attctgctgg aggtagtatt gttgatataa atcttgaact tatgcagatt
   101641 acacctttac atccgataaa taccaatgca tataggtgct ttacatcaag tataatatgt
   101701 tcaaatgaat ctgttataaa gattgcacca gaatatagat ttgaagattc tgtattgatt
   101761 gtcgttgatg gagttgagca cagatttaga caaatcgaaa atataaaagt tagtatatct
   101821 gatgcaaaaa taaaattact tagaatgtca aactatgagt tttggcatag agtatctgaa
   101881 aaatttttat agactgtact taggaggatt tttaatatga aaataggaat aatgagtgat
   101941 actcatggta gcttacttta ctttgaaaaa gcgttaaatg tattatctga ttgtgatgtt
   102001 ttattacatg gtggagatgt cttatatcat ggacctagaa atgatattcc agaaggatat
   102061 aatcctaaaa aatttattga gacattaaat aaattagaaa acattgtgat tgtaaaaggg
   102121 aactgtgatg cagatgttga ccagatggtt atagaacatc ctattcagag tccttatgtc
   102181 atgagccaat ttggtgaaat tagaattata ttgaatcatg gttatataga atcagaagaa
   102241 gaaattattg acaaagcaaa gaaaatgggt ggtgacatat tagttttagg acatactcat
   102301 gtaaaaaaac tatacatgga tgataattta atagtaataa atccaggaag tacttctatc
   102361 ccaaaagatg gaagccattc tgtcgcaatt atagatatca taaaaactga tgatgaggat
   102421 gagcttgaac ttgatataaa cttaattgac atcaatactg gaaatataat aaatatcaat
   102481 gattagtaaa tttttaataa aaataaaata agctttaatt tattaaattc ataataaaga
   102541 caagtaaacc attttatttc tatctcaatt agaagtttaa gtacaatacc ttaaacttct
   102601 aattaaatag aatctggttt acttgtttta ttatatgcta tattgaaata ta
//
LOCUS       DAMJWU010000005        99786 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMJWU010000005 DAMJWU010000000
VERSION     DAMJWU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 99786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.005"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..927
                     /locus_tag="PC460_000448"
     CDS             <1..927
                     /locus_tag="PC460_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2599355.1"
                     /translation="SVTGNKVGFVKIQLGDGGGSEYTPTESQTALKNVVWEGNIGNTT
                     TDETAPNCIILESLIPSSVGGFMIREIGYLDDENNLIAISKYKECYKPSIEQGAVVDM
                     KVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETKIGTVNTKIDTTKTELTSNIETA
                     KTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESGFKATVTHSLLTQRIVVNIIDAT
                     TKENIVPNFKIVDDNSIEIRSEVKVELNVYVINGNAETHFINATVDDNRVSEMTTYSS
                     KKIEDRLVNIEEKVNGGLSNIATSVNELITYC"
     gene            944..2644
                     /locus_tag="PC460_000449"
     CDS             944..2644
                     /locus_tag="PC460_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599356.1"
                     /translation="MQTEWNFNYANYVQNVSLPPGRYKLECWGACGGAVDTSDWTDCA
                     KGGYSKGEIVFKKRTNLQICVGQSGYEKVSEGSSLTRSGFNGAGAAGKVTTGSFAYSK
                     YGGGATDIRLYHPSATWGNTESLLSRILVAGGGGGMKNNFASARSIGHGGGYVGVNGV
                     GRDRDFCGGGSQYQGGTSYDTEEYHGSLGKGGYGNIGIGGGGGWYGGAGSYSNECGGG
                     GSGYALNKDSYKVPGYIPTPEYYLENIVMTTGGNTTKADGYAKITLLQALPFLTVSSY
                     NSTQATFKADHTDPALLTKIEWFIDDVLKETITTNLTEEKTINYTLEDNALHTLKIVV
                     TDSNNATAEKAVSISKNIMPLPENVNLNDISTKLVEVNAGFKTGKTSIINTLALKNIE
                     ASLNNTLVELSEKIKTSFDSSDASVQDLMNQLTQANNTISQLNSKYKVASGNSVVNYC
                     TNTKGFYFNSDYLFLFPGAIQINGLNFVPNIFFTTFELIDDGYFHKYFVFACRGIFTQ
                     DFVITAHYYRLTSYLQDFKVGGEVLKLNERDVYMDNRGIQLPCSRQGSSFKWQAIKFI
                     "
     gene            2661..2954
                     /locus_tag="PC460_000450"
     CDS             2661..2954
                     /locus_tag="PC460_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599357.1"
                     /translation="MDRANRIIYDQTGKILLQTGEATGDILEHDTITELHYIDVEYGS
                     IDYTRNRIIGINIETKEPILEEIPVFISEEEKRIQELENQILLNENKKVGGIL"
     gene            2954..3136
                     /locus_tag="PC460_000451"
     CDS             2954..3136
                     /locus_tag="PC460_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599358.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNAEYRKAVED
                     YIIRESGVVEEAEPTI"
     gene            3169..4401
                     /locus_tag="PC460_000452"
     CDS             3169..4401
                     /locus_tag="PC460_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599359.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKEVFQN
                     GVNAKSNVVTALNSKGADVTTSDTWEEIKNKIDIKEGRLDLRETTLSNNYSYLVTNGA
                     IKYIERCSGNFKTFEYEEPYFYVIKETHLIKINAIDETVVFDITLANANFSCICVTQE
                     YLFISDNTKLYKINKITGIEVQSIEGAYYKLCTYGEFIYGVYGDETSSTLHKIRISDM
                     YIMLTKNLVSNGIYNFKGGKFVCNKNAIYATTEHSNSSSITTCHLSKINFDFAIAKDF
                     RIGGYLHMKNIKFLNDFVIVSDAERGIEIDSNKKSALVKYDANLNLIVYSDDNKYDNF
                     DIYNGYIYAIYSLSSSPFVKISLNTLKHIDSYRKLTETYPNAGMFVINDIVFFISSGI
                     FRNILSKKVYSDEKGESL"
     gene            4398..4697
                     /locus_tag="PC460_000453"
     CDS             4398..4697
                     /locus_tag="PC460_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599360.1"
                     /translation="MIYLGNLMDTEEQNIKYVGMIHYEPNLLSEENLKQGILIESLPT
                     QKYAENKEAKLFINIDTKEVFYRYTDIKSNIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            4699..4836
                     /locus_tag="PC460_000454"
     CDS             4699..4836
                     /locus_tag="PC460_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDF2599361.1"
                     /translation="MNWYKIIADFYNNGNWTKEQVKTAVEKNKITASEYKEITGEDYI
                     A"
     gene            4868..5098
                     /locus_tag="PC460_000455"
     CDS             4868..5098
                     /locus_tag="PC460_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDF2599362.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLIGFFIFAIQRGIF"
     gene            5114..5371
                     /locus_tag="PC460_000456"
     CDS             5114..5371
                     /locus_tag="PC460_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2599363.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPIILLVL
                     GIGFSIWMLGLSPNAVLQGIICWGISIGINQTYKQLKEENK"
     gene            5371..6183
                     /locus_tag="PC460_000457"
     CDS             5371..6183
                     /locus_tag="PC460_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2599364.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKNEEKSYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVVGGGACEKISSITKEKFIMIKGNDRFDTLYKALDFINR"
     gene            6264..6626
                     /locus_tag="PC460_000458"
     CDS             6264..6626
                     /locus_tag="PC460_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599365.1"
                     /translation="MEDLCMDALVYERFVRAAFSIKLNNLINRSEDLGGLAEADIFRA
                     ANNLHELNEIKIGTGYAIAIFNNEILESCNVSDNDSNRMIELFDRSLIATSREEILDI
                     IREYETYRGRYLTFNWKR"
     gene            6792..7310
                     /locus_tag="PC460_000459"
     CDS             6792..7310
                     /locus_tag="PC460_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599366.1"
                     /translation="MDKKIDIAKFIVNRIDYYIEKSDNKASFLLLLNSAIIGFLFSGK
                     EKIMNHLSISNIRCLEMLFYIVILCIFVISIYFSIMVLKPRNSKIENEYKSIFYYKEI
                     ASLNNEQYKEAFENAFKDEENLINDALVQIKELSLICDKKMYNVKKSVEAFILGGIIL
                     FIYILVVVFNAI"
     gene            complement(7374..8960)
                     /locus_tag="PC460_000460"
     CDS             complement(7374..8960)
                     /locus_tag="PC460_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599367.1"
                     /translation="MVRIFTMVDDKEYKTQNVNNVHNSAKNENAFNLKKYKEKLQENI
                     NTDIYEKEKEPNHPEVILFFSFDIANSSLYKNINYSGWAKVLSHIIRKLQYRVYENLK
                     AQLWRVLGDEVIFIIVLKNYDEIYKYIDIIFDILTSTAKDIKSGNIFSTLEGFSESEK
                     YLMKLQNIISLKGAAWIAIVSRNPNFNALENNEQYENISAMYDLSNNYKIFEFLGNDI
                     DAGFRISKQTCPERLVLSFELAYILSRKTDILSKLHIITYKKLKGIWKDKLYPIIWYH
                     NKGKNNDIEFDDSFSFDEIEENELVREYFFNKKGESKLLIDSFMFNSVDKALDKILID
                     RNLSDKIEKIGDVISKTNPSYDKNTIDKDYIKVDLMELHCVAVCYNKSTSKILIAKRS
                     DNRNNNASKWEFGCAKASLETSIINTIKDEYEKDFNINIEPITDCTRKDDCQPIPLAI
                     YQVKKSDGLHKGIITLAEIINDYDISKFEPTSKHNELAWIGEDELEDFNENTVPDFKE
                     TLKLAFKKLNENQLQESTNM"
     gene            complement(8936..9457)
                     /locus_tag="PC460_000461"
     CDS             complement(8936..9457)
                     /locus_tag="PC460_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF2599368.1"
                     /translation="MDFKIRELINDITQDIIQTYKIQIPIVNINQVVDALGGKVIEDS
                     SLSGYSDGFIRKVDDSFEIVVSPYQPDTRKNFTIAHELGHLFLHMGYGIDDELWNSQD
                     GNQYFRSGNTNKEYQSNEFAAALLMPKHEYKRIMDENTVGNKVDTSKIAEYFNVSSYA
                     ASNRGKWLGYLQW"
     gene            complement(10159..10347)
                     /locus_tag="PC460_000462"
     CDS             complement(10159..10347)
                     /locus_tag="PC460_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599369.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            10601..10777
                     /locus_tag="PC460_000463"
     CDS             10601..10777
                     /locus_tag="PC460_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599370.1"
                     /translation="MKTLMFILVIAYYITIVIKSITAIVKSIIRLLDSINRLKESLHK
                     LRNRPDCNQDDFNE"
     gene            11298..11687
                     /locus_tag="PC460_000464"
     CDS             11298..11687
                     /locus_tag="PC460_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2599371.1"
                     /translation="MEQQDVIDKLRRGELMLMEYLWNKKSILSKKEIIEAMKKRYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNARKEERDISKYDNFFARIF
                     TTIHKRDKMTEEQLKAFIKNMEKIGKI"
     gene            11780..12160
                     /locus_tag="PC460_000465"
     CDS             11780..12160
                     /locus_tag="PC460_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2599372.1"
                     /translation="MGFKKLPDSELKVMKFIWGLEKKVASGEVVEAMKQTYGWNKNTT
                     LTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTALN
                     DEENLSDDKLDMLEEWVKNWEEDE"
     gene            12839..14269
                     /locus_tag="PC460_000466"
     CDS             12839..14269
                     /locus_tag="PC460_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2599373.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(14417..14767)
                     /locus_tag="PC460_000467"
                     /pseudo
     CDS             complement(14417..14767)
                     /locus_tag="PC460_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14762..15283
                     /locus_tag="PC460_000468"
     CDS             14762..15283
                     /locus_tag="PC460_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2599374.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(15412..16206)
                     /locus_tag="PC460_000469"
     CDS             complement(15412..16206)
                     /locus_tag="PC460_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2599375.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(16430..19858)
                     /gene="uvrA"
                     /locus_tag="PC460_000470"
     CDS             complement(16430..19858)
                     /gene="uvrA"
                     /locus_tag="PC460_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2599376.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHQDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLKGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            20177..22411
                     /locus_tag="PC460_000471"
     CDS             20177..22411
                     /locus_tag="PC460_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2599377.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            22667..24697
                     /locus_tag="PC460_000472"
     CDS             22667..24697
                     /locus_tag="PC460_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2599378.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            24694..25146
                     /locus_tag="PC460_000473"
     CDS             24694..25146
                     /locus_tag="PC460_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2599379.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            25148..26203
                     /locus_tag="PC460_000474"
     CDS             25148..26203
                     /locus_tag="PC460_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF2599380.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            26270..26578
                     /locus_tag="PC460_000475"
     CDS             26270..26578
                     /locus_tag="PC460_000475"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF2599381.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            26682..27947
                     /locus_tag="PC460_000476"
     CDS             26682..27947
                     /locus_tag="PC460_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF2599382.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            28035..28751
                     /locus_tag="PC460_000477"
     CDS             28035..28751
                     /locus_tag="PC460_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2599383.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(28946..29239)
                     /locus_tag="PC460_000478"
     CDS             complement(28946..29239)
                     /locus_tag="PC460_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF2599384.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(29245..29460)
                     /locus_tag="PC460_000479"
     CDS             complement(29245..29460)
                     /locus_tag="PC460_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599385.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAMQQFIVENEILISEEV"
     gene            29909..30307
                     /gene="tnpA"
                     /locus_tag="PC460_000480"
     CDS             29909..30307
                     /gene="tnpA"
                     /locus_tag="PC460_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF2599386.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            30304..31467
                     /locus_tag="PC460_000481"
     CDS             30304..31467
                     /locus_tag="PC460_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2599387.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(31851..32363)
                     /locus_tag="PC460_000482"
     CDS             complement(31851..32363)
                     /locus_tag="PC460_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2599388.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            32813..33289
                     /gene="purE"
                     /locus_tag="PC460_000483"
     CDS             32813..33289
                     /gene="purE"
                     /locus_tag="PC460_000483"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF2599389.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            33295..33993
                     /locus_tag="PC460_000484"
     CDS             33295..33993
                     /locus_tag="PC460_000484"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2599390.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            34005..35372
                     /gene="purF"
                     /locus_tag="PC460_000485"
     CDS             34005..35372
                     /gene="purF"
                     /locus_tag="PC460_000485"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF2599391.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            35366..36442
                     /locus_tag="PC460_000486"
     CDS             35366..36442
                     /locus_tag="PC460_000486"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF2599392.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            36436..37029
                     /locus_tag="PC460_000487"
     CDS             36436..37029
                     /locus_tag="PC460_000487"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF2599393.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            37022..38554
                     /gene="purH"
                     /locus_tag="PC460_000488"
     CDS             37022..38554
                     /gene="purH"
                     /locus_tag="PC460_000488"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF2599394.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAVVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            38565..39815
                     /gene="purD"
                     /locus_tag="PC460_000489"
     CDS             38565..39815
                     /gene="purD"
                     /locus_tag="PC460_000489"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF2599395.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTRVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            39840..43646
                     /locus_tag="PC460_000490"
     CDS             39840..43646
                     /locus_tag="PC460_000490"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF2599396.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDNKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVIGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            43852..44724
                     /gene="rfbD"
                     /locus_tag="PC460_000491"
     CDS             43852..44724
                     /gene="rfbD"
                     /locus_tag="PC460_000491"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2599397.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            44833..45702
                     /gene="rfbA"
                     /locus_tag="PC460_000492"
     CDS             44833..45702
                     /gene="rfbA"
                     /locus_tag="PC460_000492"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2599398.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            45751..46308
                     /gene="rfbC"
                     /locus_tag="PC460_000493"
     CDS             45751..46308
                     /gene="rfbC"
                     /locus_tag="PC460_000493"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2599399.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            46346..47329
                     /gene="rfbB"
                     /locus_tag="PC460_000494"
     CDS             46346..47329
                     /gene="rfbB"
                     /locus_tag="PC460_000494"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2599400.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            47359..48027
                     /locus_tag="PC460_000495"
     CDS             47359..48027
                     /locus_tag="PC460_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2599401.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            48036..48464
                     /locus_tag="PC460_000496"
     CDS             48036..48464
                     /locus_tag="PC460_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF2599402.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            48488..48802
                     /locus_tag="PC460_000497"
     CDS             48488..48802
                     /locus_tag="PC460_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2599403.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            48954..49229
                     /gene="flgM"
                     /locus_tag="PC460_000498"
     CDS             48954..49229
                     /gene="flgM"
                     /locus_tag="PC460_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF2599404.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            49234..49650
                     /locus_tag="PC460_000499"
     CDS             49234..49650
                     /locus_tag="PC460_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF2599405.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            49668..50978
                     /gene="flgK"
                     /locus_tag="PC460_000500"
     CDS             49668..50978
                     /gene="flgK"
                     /locus_tag="PC460_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF2599406.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            50997..51929
                     /gene="flgL"
                     /locus_tag="PC460_000501"
     CDS             50997..51929
                     /gene="flgL"
                     /locus_tag="PC460_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF2599407.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            51945..52337
                     /locus_tag="PC460_000502"
     CDS             51945..52337
                     /locus_tag="PC460_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF2599408.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDSGC"
     gene            52331..52543
                     /gene="csrA"
                     /locus_tag="PC460_000503"
     CDS             52331..52543
                     /gene="csrA"
                     /locus_tag="PC460_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF2599409.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            52565..52960
                     /gene="fliS"
                     /locus_tag="PC460_000504"
     CDS             52565..52960
                     /gene="fliS"
                     /locus_tag="PC460_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2599410.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            52960..53316
                     /gene="fliS"
                     /locus_tag="PC460_000505"
     CDS             52960..53316
                     /gene="fliS"
                     /locus_tag="PC460_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2599411.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            53337..54860
                     /gene="fliD"
                     /locus_tag="PC460_000506"
     CDS             53337..54860
                     /gene="fliD"
                     /locus_tag="PC460_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF2599412.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            54863..55198
                     /gene="fliT"
                     /locus_tag="PC460_000507"
     CDS             54863..55198
                     /gene="fliT"
                     /locus_tag="PC460_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF2599413.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            55300..56172
                     /locus_tag="PC460_000508"
     CDS             55300..56172
                     /locus_tag="PC460_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF2599414.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            56265..58406
                     /locus_tag="PC460_000509"
     CDS             56265..58406
                     /locus_tag="PC460_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2599415.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            58430..60307
                     /locus_tag="PC460_000510"
     CDS             58430..60307
                     /locus_tag="PC460_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2599416.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            60300..62414
                     /locus_tag="PC460_000511"
     CDS             60300..62414
                     /locus_tag="PC460_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2599417.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            62423..63418
                     /locus_tag="PC460_000512"
     CDS             62423..63418
                     /locus_tag="PC460_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF2599418.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            63510..64742
                     /locus_tag="PC460_000513"
     CDS             63510..64742
                     /locus_tag="PC460_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2599419.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            64762..65706
                     /locus_tag="PC460_000514"
     CDS             64762..65706
                     /locus_tag="PC460_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF2599420.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            65800..66207
                     /locus_tag="PC460_000515"
     CDS             65800..66207
                     /locus_tag="PC460_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HDF2599421.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            66294..67385
                     /locus_tag="PC460_000516"
     CDS             66294..67385
                     /locus_tag="PC460_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF2599422.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     regulatory      67694..67784
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            68193..68510
                     /gene="flgB"
                     /locus_tag="PC460_000517"
     CDS             68193..68510
                     /gene="flgB"
                     /locus_tag="PC460_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF2599423.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            68518..68922
                     /gene="flgC"
                     /locus_tag="PC460_000518"
     CDS             68518..68922
                     /gene="flgC"
                     /locus_tag="PC460_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF2599424.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            68932..69249
                     /gene="fliE"
                     /locus_tag="PC460_000519"
     CDS             68932..69249
                     /gene="fliE"
                     /locus_tag="PC460_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF2599425.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            69274..70824
                     /gene="fliF"
                     /locus_tag="PC460_000520"
     CDS             69274..70824
                     /gene="fliF"
                     /locus_tag="PC460_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF2599426.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            70837..71907
                     /gene="fliG"
                     /locus_tag="PC460_000521"
     CDS             70837..71907
                     /gene="fliG"
                     /locus_tag="PC460_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF2599427.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            71900..72631
                     /locus_tag="PC460_000522"
     CDS             71900..72631
                     /locus_tag="PC460_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF2599428.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            72635..73954
                     /gene="fliI"
                     /locus_tag="PC460_000523"
     CDS             72635..73954
                     /gene="fliI"
                     /locus_tag="PC460_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF2599429.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            73985..74431
                     /gene="fliJ"
                     /locus_tag="PC460_000524"
     CDS             73985..74431
                     /gene="fliJ"
                     /locus_tag="PC460_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF2599430.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            74460..75665
                     /locus_tag="PC460_000525"
     CDS             74460..75665
                     /locus_tag="PC460_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF2599431.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            75683..76291
                     /locus_tag="PC460_000526"
     CDS             75683..76291
                     /locus_tag="PC460_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF2599432.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            76318..77301
                     /locus_tag="PC460_000527"
     CDS             76318..77301
                     /locus_tag="PC460_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF2599433.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            77314..77517
                     /locus_tag="PC460_000528"
     CDS             77314..77517
                     /locus_tag="PC460_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF2599434.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            77517..78335
                     /locus_tag="PC460_000529"
     CDS             77517..78335
                     /locus_tag="PC460_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF2599435.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            78325..79020
                     /locus_tag="PC460_000530"
     CDS             78325..79020
                     /locus_tag="PC460_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2599436.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            79032..79475
                     /locus_tag="PC460_000531"
     CDS             79032..79475
                     /locus_tag="PC460_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF2599437.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIADLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            79490..79864
                     /locus_tag="PC460_000532"
     CDS             79490..79864
                     /locus_tag="PC460_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HDF2599438.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            79865..80530
                     /gene="fliP"
                     /locus_tag="PC460_000533"
     CDS             79865..80530
                     /gene="fliP"
                     /locus_tag="PC460_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF2599439.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            80543..80812
                     /gene="fliQ"
                     /locus_tag="PC460_000534"
     CDS             80543..80812
                     /gene="fliQ"
                     /locus_tag="PC460_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF2599440.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            80824..82638
                     /locus_tag="PC460_000535"
     CDS             80824..82638
                     /locus_tag="PC460_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF2599441.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNNIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            82647..84722
                     /gene="flhA"
                     /locus_tag="PC460_000536"
     CDS             82647..84722
                     /gene="flhA"
                     /locus_tag="PC460_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF2599442.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGDLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            84697..85713
                     /gene="flhF"
                     /locus_tag="PC460_000537"
     CDS             84697..85713
                     /gene="flhF"
                     /locus_tag="PC460_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF2599443.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            85710..86588
                     /locus_tag="PC460_000538"
     CDS             85710..86588
                     /locus_tag="PC460_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF2599444.1"
                     /translation="MIDQAQILRKIAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            86602..87303
                     /locus_tag="PC460_000539"
     CDS             86602..87303
                     /locus_tag="PC460_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF2599445.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            87319..87741
                     /locus_tag="PC460_000540"
     CDS             87319..87741
                     /locus_tag="PC460_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2599446.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            87726..87911
                     /locus_tag="PC460_000541"
     CDS             87726..87911
                     /locus_tag="PC460_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2599447.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            87952..88725
                     /gene="flgG"
                     /locus_tag="PC460_000542"
     CDS             87952..88725
                     /gene="flgG"
                     /locus_tag="PC460_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2599448.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            88746..89507
                     /gene="flgG"
                     /locus_tag="PC460_000543"
     CDS             88746..89507
                     /gene="flgG"
                     /locus_tag="PC460_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2599449.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            89530..90411
                     /locus_tag="PC460_000544"
     CDS             89530..90411
                     /locus_tag="PC460_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2599450.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            90414..90788
                     /locus_tag="PC460_000545"
     CDS             90414..90788
                     /locus_tag="PC460_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2599451.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            90820..92223
                     /locus_tag="PC460_000546"
     CDS             90820..92223
                     /locus_tag="PC460_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2599452.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            92339..94276
                     /gene="htpG"
                     /locus_tag="PC460_000547"
     CDS             92339..94276
                     /gene="htpG"
                     /locus_tag="PC460_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2599453.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            94663..95793
                     /locus_tag="PC460_000548"
     CDS             94663..95793
                     /locus_tag="PC460_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2599454.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            96020..97042
                     /gene="splB"
                     /locus_tag="PC460_000549"
     CDS             96020..97042
                     /gene="splB"
                     /locus_tag="PC460_000549"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2599455.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            97224..98135
                     /locus_tag="PC460_000550"
     CDS             97224..98135
                     /locus_tag="PC460_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2599456.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            98150..98566
                     /locus_tag="PC460_000551"
     CDS             98150..98566
                     /locus_tag="PC460_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2599457.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(98666..99058)
                     /locus_tag="PC460_000552"
     CDS             complement(98666..99058)
                     /locus_tag="PC460_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599458.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            99199..99594
                     /locus_tag="PC460_000553"
     CDS             99199..99594
                     /locus_tag="PC460_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2599459.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        39862 a        10654 c        17592 g        31678 t
ORIGIN      
        1 agtgtcacag gtaataaagt gggatttgta aaaattcaac ttggtgatgg aggagggagt
       61 gaatatactc caactgagag tcagacagct ctcaaaaatg tggtatggga aggcaatatc
      121 ggaaatacaa ctacagatga aactgcacca aattgtataa tattagagag tttaatacca
      181 tcaagtgtag gcgggtttat gataagagaa ataggatatt tagatgatga aaataattta
      241 attgccattt ctaaatacaa agagtgttat aaaccttcta tagaacaagg tgcagtggta
      301 gacatgaagg ttaaaactgt gcttattgta tctaatgtaa ataatataga acttaaaatt
      361 gacccaacaa taatctttgc aacactcaaa gatatacaag acttagaaac taaaataggt
      421 actgttaata ctaaaattga tacaactaaa acagaattaa caagcaacat agaaactgct
      481 aaaacagagt taaatactaa aatcgaccaa ttaatcgcag gtggctcaaa tgttgcatac
      541 actcaaaggg tcgcaattga tgattgggtt gaggatgcag aaagtggatt caaagcaact
      601 gtaacacata gtttattaac acagagaata gttgtaaata ttatagatgc tactacaaaa
      661 gaaaatatag ttccaaattt taaaatagtt gatgataatt caattgagat tagaagtgaa
      721 gtaaaagttg agttaaatgt ctatgtgata aatggaaatg cagaaactca ttttataaat
      781 gcaactgtag atgataacag agtatctgaa atgactactt attcatctaa gaaaatagag
      841 gacagattgg ttaatataga agaaaaggta aatggtggtt tatctaatat tgcaacaagt
      901 gtaaatgagt tgataactta ttgttagaga ggagagtgag aaaatgcaga ctgaatggaa
      961 ttttaattat gctaattatg tacaaaatgt ttcattgcca cctgggcgat ataaattaga
     1021 atgttggggt gcttgtggtg gtgctgtcga tacaagcgat tggactgatt gtgcaaaagg
     1081 tggttattca aaaggtgaga ttgtatttaa aaaaagaact aatctacaaa tttgtgtcgg
     1141 tcaatccggt tatgagaaag tttctgaagg ttcaagcctt actagaagtg gttttaacgg
     1201 tgcaggcgct gctggcaaag ttactactgg tagctttgct tattctaaat acggtggtgg
     1261 agcaactgat ataagacttt atcatcctag tgcaacttgg ggtaacaccg aaagtttgct
     1321 ttcacgcata cttgttgcag gcggtggagg aggtatgaaa aataattttg cttctgctcg
     1381 ttctattggt catggtggtg gttatgtagg tgttaatgga gttggtcgtg acagagattt
     1441 ttgtggcggt ggttctcaat accaaggtgg aacaagttac gacacagaag aataccatgg
     1501 ttcattagga aaaggaggtt atggtaacat aggaataggt ggtggagggg gttggtacgg
     1561 tggtgctggt tcttattcta atgaatgtgg aggtggtgga agtggttacg cactaaataa
     1621 agatagttat aaggtacccg gatatatacc aacacctgaa tattatcttg aaaatatagt
     1681 catgactact ggaggtaata ctactaaagc agatggttat gctaaaataa cattactaca
     1741 agcattgcca tttttaacag tatcttctta taattctaca caggcaacat ttaaagccga
     1801 ccacacagac cctgcattac ttacaaagat agaatggttt atagatgatg tactaaaaga
     1861 aactataaca acaaatttaa cagaagagaa aacaattaat tatacattag aagataatgc
     1921 attacacaca cttaaaatag ttgttacaga cagtaataat gctacagcag aaaaagctgt
     1981 cagtataagt aagaatataa tgccattgcc cgaaaatgtt aatttaaatg atatatctac
     2041 taaactagtt gaagttaatg caggatttaa gactggtaaa acaagtatta taaacacttt
     2101 ggcactaaag aatatagaag caagtttaaa taacacatta gtcgagttgt cagagaaaat
     2161 aaaaacaagt tttgatagtt cagacgctag tgtacaggat ttgatgaacc agttaacaca
     2221 agctaataac actataagtc agttaaattc taaatataaa gttgctagtg gcaatagtgt
     2281 tgttaattat tgtactaata caaaaggatt ttattttaat agtgactatc tgttcctatt
     2341 tcctggagcc attcaaatta acggacttaa ttttgttcct aatatatttt ttactacttt
     2401 tgaattaatt gatgatggtt attttcataa atattttgtt tttgcttgtc gtggtatttt
     2461 cactcaagat tttgtaatta ctgctcatta ttatcgtcta acatcatatt tacaagattt
     2521 taaagttgga ggtgaggttc ttaaattaaa tgaacgtgat gtttatatgg ataatagagg
     2581 tatacaactt ccttgttcaa gacaaggttc ttcttttaaa tggcaagcta taaaatttat
     2641 ttaataaatg aggtgataaa atggatagag caaatagaat aatttacgac cagacaggca
     2701 aaatactgct acagacaggg gaagcaacag gagatatact agaacatgat acaataacag
     2761 aattacatta tattgatgtt gagtatggaa gtatagacta tacaagaaat agaattatag
     2821 gtataaatat agaaacaaaa gaaccaattt tagaagaaat accagtattt atctcggagg
     2881 aagaaaaaag aatacaagag ttagaaaatc aaattttatt aaatgaaaat aagaaagtag
     2941 gaggaatttt ataatgaata taaataatgt tgtggtaaga atattagcag agagaatatt
     3001 aaacggaggc ttaaaccctt taaaaaatcg agaatttgaa ctggatgatg taactaatgc
     3061 agaatacaga aaagcagtag aggattatat aattagagaa agtggagtag tcgaagaagc
     3121 agaaccaact atatagaggg ttctttttta ttgaaagaag gtgactaaat gacttttaaa
     3181 gagttagtta ataaagttag aaatcttgta ttagaagcaa agaatgtaac tatagaagat
     3241 acagaaaata actttacaag tgataatgta gaaggagcat tgaaagaggt ttttcaaaat
     3301 ggagttaatg ctaaaagtaa tgtagtaaca gcattaaact ccaaaggtgc agatgttact
     3361 acaagcgata catgggaaga aataaagaat aaaattgata taaaagaggg gagattagat
     3421 ttaagagaaa caacactttc aaataactat tcgtatttag ttacaaatgg agctataaaa
     3481 tatattgaaa gatgtagtgg gaacttcaaa acttttgaat atgaagaacc atatttttat
     3541 gtaattaaag aaactcatct aattaaaatt aatgctattg atgaaacagt agtttttgac
     3601 attactttag ctaatgctaa cttctcatgt atctgtgtta ctcaagagta tttatttata
     3661 tctgacaata ctaaattata taaaataaac aagataacag gaattgaagt gcagtcaata
     3721 gaaggtgctt attataagtt atgtacttat ggggaattta tttatggagt atatggagat
     3781 gagacttctt ctacacttca taaaattaga atatctgata tgtatataat gctaactaaa
     3841 aatttagttt ctaatggtat ttacaatttt aaaggaggta agtttgtttg caataagaat
     3901 gctatttatg ctacaacaga acactcaaat tcaagtagta ttacaacatg tcatttatct
     3961 aaaataaatt ttgattttgc cattgctaaa gattttagaa ttggaggata tttgcatatg
     4021 aaaaacatta agtttttaaa tgattttgtt attgtatctg atgcagaaag aggtatagaa
     4081 attgatagca acaaaaaaag tgctttagta aaatatgacg caaatttaaa tttaattgtg
     4141 tattcagacg ataacaaata tgacaatttt gatatatata atggatatat atatgctata
     4201 tattcgcttt ctagtagtcc ttttgtaaaa ataagtttaa atactcttaa acatatcgac
     4261 agctaccgaa aacttactga aacataccca aatgctggta tgtttgtaat aaatgatata
     4321 gttttcttta ttagtagtgg aatttttaga aatatattgt caaaaaaggt ttattcggat
     4381 gagaaaggag aatcattatg atttatttag gaaatttaat ggatacagaa gaacaaaata
     4441 taaaatatgt tggtatgata cactatgaac caaatttgtt atcggaggaa aacttaaaac
     4501 aaggtatttt gatagagagt ttaccaactc aaaagtatgc agagaataaa gaagcaaagc
     4561 tatttataaa tatagatact aaagaggttt tctatagata tacagatatt aaaagtaaca
     4621 tagaagataa agtaaattct acagaacaaa caatagcaga tttaacattt caattaatga
     4681 gtaatggggt gatatagtat gaattggtat aagataatag cagatttcta taataatggt
     4741 aattggacta aagagcaagt taaaacagca gtagaaaaga ataagataac agcaagtgaa
     4801 tataaagaaa taacaggaga ggactatata gcatagtctt ttttaattca aaaattagga
     4861 ggttttcatg aatgaagaac ttttcgaagc agatttaaaa agacatgaaa caagaataaa
     4921 taaacatgga gaagaaatag acgaattaaa aatagcaaat atagagtcta aagcagaatt
     4981 aaaagcattg tgtgagaatc taaactcact tacaagtatg ctcaaatggc taattggtac
     5041 aatgattaca acacttatag ggttctttat atttgcaata caaagaggaa tattttaatt
     5101 aggaggataa aagatggata atttaataag ttttatacca gagcagttgc taattttagt
     5161 ggctgctctc tctattatag gtaaaggttg taaaaaatat aaacaattag ataataaata
     5221 tattccaatt atattacttg tgttgggaat cggattttct atttggatgc tagggcttag
     5281 tcctaacgca gtcttacagg gaataatttg ttggggaata tcaataggta taaatcaaac
     5341 ttacaaacag ttgaaggagg aaaataaata atgaaaatat gtataacagt aggacacagt
     5401 attttaaaaa gtggagcatg tacttctgct gatggagtag ttaacgagta tcaatacaac
     5461 aaatctcttg caccagtatt agcagataca tttagaaaag aagggcataa ggtagatgta
     5521 ataatatgcc cagaaaagca gtttaaaact aagaatgaag aaaagtctta taaaatacct
     5581 agagttaata gtggaggata tgatttactt atagagttac atttaaatgc aagtaacggt
     5641 caaggtaaag gttcagaagt cctatattat agtaataaag gcttagagta tgcaactaga
     5701 atatgtgata aactaggtac agtatttaaa aatagaggtg ctaaattaga taaaagatta
     5761 tatatcttaa atagttcaaa gcctacagca gtattaattg aaagtttctt ctgtgataat
     5821 aaagaagatt atgataaagc taagaaacta ggtcatgaag gtattgctaa gttaattgta
     5881 gaaggtgtat taaataaaaa tataaataat gagggagtta aacagatgta caaacataca
     5941 attgtttatg atggagaagt tgacaaaatc tctgcaactg tagttggttg gggttataat
     6001 gatgggaaaa tactgatatg tgatataaaa gattacgtgc caggtcagac gcaaaatctt
     6061 tatgttgtag gaggtggcgc atgtgaaaag ataagttcta ttactaaaga aaaatttatt
     6121 atgataaaag gtaatgatag atttgataca ctttataaag cattggattt tattaataga
     6181 tagataaaag ttatcaacta gaagtggttg tttgttgtga taactctatt attgtaatat
     6241 aatgtaaata tattattgtg ataatggagg atttatgtat ggatgcattg gtttatgaga
     6301 gatttgtaag agcagctttt agtattaaac ttaataactt gattaataga agcgaagatt
     6361 taggtggatt ggctgaagct gatatcttta gagcagcaaa taatttacat gaattaaatg
     6421 agataaaaat aggtactggg tatgctattg caatatttaa taatgagata ttagagtctt
     6481 gtaatgtttc tgataatgat agcaatagaa tgattgaact atttgataga agtttaattg
     6541 caacttctag ggaggaaata ttggatatta taagagaata tgaaacttat agaggacgat
     6601 atcttacttt taattggaag agatagaatg tttaagttag gaagtagtaa ttatagttag
     6661 ttattacttt ctttcacata tttttattat ttcaataaga ttatttccaa ataataattt
     6721 attcaatgat aataagtaga aaataaaatt ttgaatcaat ataaaattta atgaaatgag
     6781 gtagtaaaat catggataaa aaaatagaca tagcgaagtt tatagtaaat agaattgatt
     6841 actatattga aaaatcagat aataaagcta gttttttact attattaaat agtgcaatta
     6901 taggtttttt attttcaggg aaggaaaaaa taatgaatca cttaagtata agtaatatca
     6961 gatgtttaga gatgctattt tacattgtta tattatgtat atttgttatt tcaatttatt
     7021 tttcgattat ggttttaaag ccaaggaatt ctaaaataga aaatgaatat aaatctattt
     7081 tttattataa agagatagca tctttaaata atgaacagta caaagaagca tttgaaaatg
     7141 catttaaaga cgaagaaaac ttgattaatg atgcacttgt tcaaataaaa gaattatctt
     7201 taatctgtga taagaaaatg tataatgtaa aaaagtcagt agaagctttt attttaggtg
     7261 gtattatatt gtttatatat atacttgtag tagtttttaa tgctatttag aaaatgtttt
     7321 atttggttag tattcctcta tgaagataga atcaaatcat agaagaatac tatttacata
     7381 tttgtagatt cttgtaattg attttcattt aattttttaa atgctagttt taaagtttct
     7441 ttaaaatcag gtactgtgtt ttcattaaaa tcttcaagtt catcctctcc tatccatgca
     7501 agttcattat gttttgatgt gggttcaaat ttagaaatat cataatcatt tattatttct
     7561 gcgagagtta taatgccttt gtgtaaacca tcactttttt taacttgata aattgctaga
     7621 ggtataggtt ggcaatcatc ttttcttgtg caatcagtaa taggttcaat gtttatatta
     7681 aaatcttttt catattcatc tttaatagta tttataattg aagtttctag acttgcctta
     7741 gcacaaccaa attcccattt actcgcatta ttatttctat tatcacttct tttcgcaatt
     7801 aatatttttg aagttgattt attataacaa accgcaacac agtgtaattc cattaaatct
     7861 acttttatat agtctttatc tattgtattt ttatcataac tggggtttgt tttagaaatt
     7921 acgtcaccta ttttttcaat cttatcacta aggtttctgt ctataagtat tttatccaaa
     7981 gccttgtcta cagaattaaa cataaaagaa tcaattagta atttactttc tcctttttta
     8041 ttaaaaaaat attctcgaac caattcattt tcttctattt catcaaaaga aaaactatca
     8101 tcaaattcta tatcattatt ttttccttta ttatgatacc aaatgatagg gtatagttta
     8161 tctttccaga taccctttaa ttttttatat gtaattatat gtaatttaga taaaatatca
     8221 gtttttcttg ataatatata agctagttca aaactaagaa caagtctttc tgggcatgtt
     8281 tgctttgata ttctgaaacc agcatcaata tcatttccta agaactcaaa tattttataa
     8341 ttattggata aatcgtacat agcagaaata ttttcatatt gttcattatt ttctaaagca
     8401 ttaaaattag gatttcttga tactatagca atccaggccg ctccttttaa tgaaataata
     8461 ttttgaagtt tcattaagta tttttcagat tcagaaaatc cttctagtgt agagaatata
     8521 ttaccacttt ttatatcttt agcagtgctt gttaaaatat caaagattat atctatgtat
     8581 ttataaattt catcataatt ttttagtacg ataatgaaaa ttacctcatc accaagaact
     8641 ctccaaagtt gtgctttaag gttttcataa actctatatt gtaacttacg aattatatgt
     8701 gataatacct tagcccatcc gctataattt atatttttat ataatgatga attagctata
     8761 tcaaaagaaa agaacaaaat cacttctgga tgattaggtt ctttttcttt ttcataaata
     8821 tcagtattaa tattttcttg aagtttttcc ttatactttt taagattaaa agcgttttcg
     8881 tttttagctg aattatgaac attatttaca ttttgagttt tatattcttt atcatctacc
     8941 attgtaaata tcctaaccat ttccctctat tagatgctgc ataagaggaa acattaaagt
     9001 attctgcaat ttttgaagtg tcgaccttat tacctactgt gttttcatcc ataattcttt
     9061 tatattcatg tttaggcatc aacaaggctg ctgcaaattc attggattga tactccttat
     9121 tggtattgcc acttctaaaa tactgatttc catcttgact attccatagt tcatcatcaa
     9181 tgccataacc catgtgtaaa aataaatgtc caagttcatg agcaatggta aaattctttc
     9241 tggtatctgg ttgataagga gataccacta tttcaaatga atcatcaact tttctaataa
     9301 atccatcaga gtatccactt aaagaactat cttctattac cttgcctcct aaagcatcaa
     9361 caacttgatt tatatttact attggaattt ggattttgta tgtttggata atatcttggg
     9421 ttatatcatt aattagctct ctgattttga aatccataaa atcacatcct tatataatat
     9481 tttatttaaa taattattta ttataatatt cattatatca aaaactattt tttttggtac
     9541 atttaatatt aattattttc attaaccttt tgtattatac aatataatta taccatataa
     9601 aaactgtttt ttgtcgaatg tatgtttgaa aaaataaaaa ttagtaaatt atcttaaaaa
     9661 taattagata tactttggaa gcaataagaa tattaacaat aagataaatc gttgaaatac
     9721 caacatgtgt taaataataa caataaacaa ataataaaat tttagatttc agaatatgaa
     9781 agaaccaaaa aacgattaac tagtaatctt aattaaattc atttagttga tgttaaagta
     9841 tgaaaactat agtagaagtt gtaataataa aacgaacgaa aattatcata ctaaaacata
     9901 agacatgcta taattgtatt agataaatgc ttgaatatat accaaaagta ctctttttat
     9961 aagagtgctt atttttttga aattcatcaa catataaact atcaagaaca ttactcaaca
    10021 caccttaaaa ttgatttaaa ttctttttca tacacaaagt tatatgatat aataaaaaag
    10081 taaatatgta accccaacac atctttacta agtcaaacat tattatatag agcattcttc
    10141 attatggaga gtgctttttc atttctttga ataatcatgt tgattattta taatattttc
    10201 atttaattta tctttatcta tcaaatttaa tgcacaatta atacaaatgt tagttttcat
    10261 atgacttttc ttatgaaaag taatatactt attatcatct ttatttattc ctttattaca
    10321 gtaatcacat aatatagtct tagtcatatt tttattcctt tcattttata ttttccataa
    10381 ttaagtttaa catatttagt atatacctat gtatatatat ttaaaacaat gttttttatt
    10441 gcaatataga atatattgta taaataaaaa aaatggagaa tagatatttg ataattgtaa
    10501 aattatgtta taattaaaat gcaagaataa tcttgcaaga aactacaatc taagggcgaa
    10561 agttttcaga aatcaccttc gtatgaaagg gggtgagtat atgaaaacac ttatgtttat
    10621 acttgttata gcgtattata ttactattgt gataaaatct attacagcaa tagtaaagtc
    10681 tataataaga ttactagatt ctataaatag attaaaagaa tctttacata aactaagaaa
    10741 tcgtcctgac tgcaatcaag acgatttcaa tgaataaatc attacttaaa ttattttgaa
    10801 tttgaaactt cactctaggc taatagattg tagtttcttt tttgtttttg atacgaattt
    10861 acatctttat tatatcgcat tttgagaaaa aataaaacta tgaatattag aaatatattt
    10921 tattttatct ctaagtatat atattattac ccacctaaaa aactttcaca aaaacaaaaa
    10981 gagtatatgt ttttgataac tggatgatat aacttataac tcattttaca attcttagtt
    11041 catatatatt tacgccacta aatttctcta gttttgccga tttagtatta ttctacaact
    11101 aaaataatac aatagaacta cttattattc aacaagattg tttgaaagtt aaataaattt
    11161 atagaaatga ataatatttt acgcttgaaa aaacagacta aatacgtaga atatttggta
    11221 aaacttacct gtattaattg caattagaag gttctattat taatttaaat taaaattaat
    11281 tcaaggaagt gacataaatg gaacaacaag atgtaataga caaactacgt aggggcgaat
    11341 taatgctaat ggagtatttg tggaataaaa agtctatttt atcaaaaaaa gaaataatag
    11401 aagcaatgaa aaagagatat aagtggagga aaagtactac agaaattctt ttaaaaagat
    11461 tggtaaagat gaaaacatta aaaaagaaga ggataggttt tcagtttagc tatgaagtat
    11521 tagtaactaa aaaagaatat ttaaatgcta gaaaagaaga aagagatata agtaaatatg
    11581 ataatttttt tgcacgaata ttcacaacta tacataaaag agataagatg acagaagaac
    11641 aactaaaggc atttataaaa aatatggaaa aaattggaaa aatataattt atatatatca
    11701 attaatttta aattaaattt gttataatat ttatatatat tttaatatgc agaaatttac
    11761 aaaaaagggg gtgcatatta tgggatttaa aaaattacct gattcagaat taaaagtaat
    11821 gaagtttatc tggggattag aaaaaaaagt agcttcagga gaagttgtag aagctatgaa
    11881 acaaacttat ggttggaata aaaatacaac actaacaata ttgtctaagc ttgcaaagaa
    11941 aaactatttg tattctcaaa aaacaagcaa gtgtacatat tatactatcg ctgtaagaga
    12001 aaaagattat ttaaaagttg aaacaaaaaa attctttagt ttttttcata ataattcttt
    12061 aaaaagtttt ttcactgctt taaatgatga agaaaattta agtgatgata aattagatat
    12121 gttagaggaa tgggtgaaaa attgggaaga agatgaataa aatgaatgtg atactaaaat
    12181 gatactaata tgttttatag cgtgttaaaa aatatagaaa atatagaaat aatagaagaa
    12241 aattaatgta atagataagg ttttatcaga ttaaaaagaa aatcaataga atgggaataa
    12301 aatatagaat gccaaaaatc cacataaata caggatttga ggtctctctt acttctatgt
    12361 tatactggtt tgatactatc aatatttgcc agtatcttat ggattaataa gcaaaaaaca
    12421 tcctgctgaa tgataaattc agcggggtgt ttttattttg ttttagaact cctttgctct
    12481 gaaactcgaa gtatttaaca ctgatgattt tgttatcttt tagttccttt tgtgtcatgc
    12541 tctgaaagac aaattgatac aataaaaaag taatttctta atcattgatg gagatatgtg
    12601 tttatgttac tcttgatggt aatttgagta atattgttca tagcttttta tattcactta
    12661 attagaaaac catttggtat aatcataaat aaacttgatg atataataga aaagatttat
    12721 aattattaga agtatataaa agtattttat gtctatgaaa tgtatgtcta taaaacatat
    12781 agatgacttt tgtatattaa tgtatgaata ttgatattat agaagaattg agttgtcaat
    12841 gatactaaat caaatattta tagaagctgg agggattatg agaaataaaa taattatagg
    12901 aattactttt tcacttgtat tactaatgtc tggatgtgct tcaaaacaaa ccgcagaaat
    12961 aaacgtatta gcaactactg atttacatgg agttatacct tatgagttaa cttcttatgt
    13021 aaaagaagaa agaaaaaaag ataaaaatct aactttagta gatgcaggag atttttttga
    13081 tagtggagaa gtgtttggtt ctagcatgga tagttatttt agtgctagat ataaagatgg
    13141 tgataataac actgaaagtt atattgagac gcctatttca aaagatatga aagaggtagg
    13201 ttatgatgtt gcagttcttg ggaatcatga atttatatcc aacaataaat tttatctaga
    13261 taatatgata tctgattttg agaagcaaaa aataaatatt ttatcggcaa atacgtataa
    13321 aaaaaatgga gatagttata caaaaccata cataataaaa aatattaata cacctgaagg
    13381 aaacgtaaaa ctaggaattt tagggcttac tataaaagaa gttggtgaga ggaaacagtg
    13441 gaaagatgga aaacttgttg actctaagtc tttagaacta aaagaccaag agggttacaa
    13501 tggtgaattg tatatgaatg atttagtaga agatgctaaa aagtgggttg gtataatgga
    13561 aaaagagaag cctgacatta tagttgctgt ggcacataca ggtgagaaac ctaagaaacc
    13621 taaaaaccca ggaaatagaa tacaagactt agctcaacaa gttgatggaa ttgatgctat
    13681 agttgcaggt cataatcatg ttcaaattga gcaacatgat tataaaaata aatcaggaga
    13741 aaatgttata gttactgagc caggaaagca tggggaatgt atttctaaga ttaattttaa
    13801 attagaaaaa aacaatgata aatggaatat agttgacaaa tctagtaaat taattaaatt
    13861 tgaaaaaaat caagaaagtg ataaatttgg agaatttatt tcttcaatta ataaagtaag
    13921 taaagataag aaagaaataa gattatcaga agtaaagact cctgaatggg ataaaatata
    13981 tgtttttaaa tctggaacat ctgcagatga tatatataaa acaataggat ataaatggag
    14041 aaatatatca tctccaaagg gtgatgacat ggtgcagatt gttgtaatga ataataataa
    14101 gcctgttagc tatgtatatg gtgaagctga agttatgcca attaatataa aatgtgatga
    14161 atcagagtat aaagataatg ttgtaactat ttatcctaat aaaaatgata aatttaaaat
    14221 tgaaaaaggt aaacaagaat atagtattaa tttaaaacat atacaataga aacttttatc
    14281 ttatttaagt ttagattgta cttaaaataa agtgtgtatt attttaaaaa ttgatgataa
    14341 ttaaaatcac aacattaaag aatagtacac actttttatg ctaaaaaata gaatgttaaa
    14401 aaattaaacc tttaaattaa agtacattgc ttatattatc caatattatt ttcaaaggtt
    14461 gtgtaatatt tttatcctta tgtaatattg ctaacatttt attttccata tctatcccat
    14521 ctaattcaat caatcgcaat tttttatgta agagtgaatc taataataaa ttttctggca
    14581 aaatggttat acctaaccct gcttgtgcag ctttaataag tgcaaaagaa ttaacacttt
    14641 cccaaacagg ataagctttt tggtttgaaa gagataatac actgtccaaa gtattgcgaa
    14701 tggcacttcc ttgctcacgt aacaataacg gaaacgtgca taactgtcgt agagataagg
    14761 cttgtttagt attggtggtg gttatgcaat catactttta atccaagaga aagtagtaaa
    14821 tcaaactgga tggataagtg agaaaatgtt tacagatatt ataactattt cacaaatgac
    14881 accagggcca ctggctgtta atacttctac ttttgttgga cttcaaattg gagggttttg
    14941 gggagcagtt gcagccactt taggttgtgt attatgtggt gtagtaatat ctctattttt
    15001 atataatttt tttcaaaagc atcagaactc tacttatata tttgaaatat taaatggtct
    15061 aaaatcagct tcattaggtt taattatctc agctgcggct acaattataa tccttgcatt
    15121 atttgggacg aataaagtgt acttcaattc tatatttgag atgctaaact ggactgcact
    15181 aataatcttt tttgttacac tactgatagt acgtaagtgg aaaattaatc ctattgtgat
    15241 tataataata agtggtatac tgggaatatt tttttattta taagtttttg gtaagtagag
    15301 gatatctatt tttttatatc ttaaatccaa atagccaata aagtaaggcc atttggattt
    15361 ttaataaatt ctgaaaagat aatcagaaac ccatataaca tttaaattat atcactggtt
    15421 ttctacaaac caatcataag cttcttggca aaatttctca ctatctatat agtaactatt
    15481 atgaacatta ttaatttcaa caagtttctt tttacatttt agtgaattat aatagctatc
    15541 aaaatattgg gttttaaaat atctatcatg aagtgtttta aagacaagag taggtatgtt
    15601 gactccatct tttatcttac tatcttttgc aataaagcat tctaacattg tattaattat
    15661 ctttgtacta tttttccaaa tatgaggatt tttcttttca aatattttat aaaattcatt
    15721 atcatagact tcattataac ctttatattt aggtggtttt actgttatgt gaaaattagg
    15781 aagaaactta aagaaaaagt ttaataattt aacagtgaga taggtatagc ctactcctgt
    15841 tgtgttttta taatcaacac aagcaaactg agcaaaactt tttaacttat tgtctgaaca
    15901 tgcataatat tgtgcaaaca taccaccaat tcccgtattc ccatatagat gtatgttgga
    15961 agaataattt ttttctatgt atgtaatgac agaatctaag tcttttacaa ttgaattgcg
    16021 agaaaaatct ttttcatatc catcactttt ccctgttcct gaaaaatcaa aagcaaatat
    16081 atttacatta tgtttggcaa gtggttcata tgctttttgc atctcaagcg tagtgacaat
    16141 aggagtatgt gtaacaacaa tattcatgga tgattttctg gtaataaatg agttacaaaa
    16201 aatcattgta tctccatttg gtatttttat atgtttccaa ctcatttgat tttcctttct
    16261 ctattataaa atataataat ttttatgttc tgttttatat attattcata ttttatcaca
    16321 gataatatac taaagttaat ttttttattg tacacctatt ataataattc ttctataata
    16381 taagacattc tataatttta tatttaagtt catgtgtatt taattgtttt tatataaaat
    16441 gtccagtaat actatttgga tttattttta tttcatcagg agttccagaa gcaacaattt
    16501 cccctccatt tattccactg tctggaccta tatcgataat ccaatcagaa gcacgtataa
    16561 cctctgtatt gtgttcaata attaaaagtg aatgctgttc atttacaagt tttctaaaag
    16621 taattaacaa attttctata tctttaaaat gaagtccagt agtaggctca tccaatatgt
    16681 acagcattcg ttttttagtt tttgtcccta attcttttgc taattttatc ctttgagatt
    16741 ctcctccact taatcctaat gtattttgtc caagctttag atatccaagt cccacttcat
    16801 ctaataagga gagttttcta tatatatcag agcaatcgct aaaaaatgtt tttgcttctg
    16861 ctacttccat gtcaagtata tctgcaattg tctttccctt gtattgtata gacagtattt
    16921 ctttttgata tctcttacct tgacattcac tacaagttac ccattggtct gccatatatt
    16981 gcagaataat ttttgtttta cctaatcctt gacagtttgg gcattgcccc tttttgctat
    17041 taaagctaaa atagctttca tccaataatt tttgttttgc ttgtggagtt tcagcaaata
    17101 gagaacgaat ttcatcaaaa atattgatat atgtagcagg tgtagaacgg ctagattttc
    17161 caataggtgt ttggtccatt aagataaaat catcaatagc atcaaatcca gtaaattcag
    17221 tataattttt atctgggata gatttttgtt tcaatttttc ttctagagca ggaagtaaac
    17281 tgtgaaatac aagagagctc ttaccagaac cactaacacc tgtaatagag cacatacagt
    17341 ttaagggaat ttctacatca atatttttta agttatttgc atggcatcct tttaagctta
    17401 accatttact tggtataatt ttattttgtg aacttatttt attgtatgtg cttaaatatt
    17461 tacctgttat agagtttgga ttttttataa tttcttgggt tgtaccttct gcaataatga
    17521 accccccttt tgtaccagca cttgggccta catcaataat ataatcagca gacagtattg
    17581 tatctttatc gtgttccaca actataacag tattaccctt atctcttagc tcttctatca
    17641 ttttgatgat taaattatgg tctcttgggt gtaaaccaat agatggttca tctaaaatgt
    17701 aggttaagcc tactaattca cttcctagct gacttgataa tcgtactctc tgcagttctc
    17761 ctccagataa agttggagct gttctatctg ttgatatata gctaagacct acctttaata
    17821 gataactaac tcgaattttt aattctgtta agatatcatt taccaaagag agctcatttt
    17881 tttgaagttg atttggtaat tcacacaacc aattccataa ctgttctatc gtcatttttg
    17941 ttagttcagg gaagcgataa cctttgattg tagtgaatct agcctcaatg cacagcaatt
    18001 cgccaccaca tgtattgcaa ggaattttgt tcatatattg atgcaaggta ttattttcag
    18061 aactagattc tctaaaaagt ctttgaatgt ggttgattgc tcctgaagca ggtcttctaa
    18121 tctttgattc tcgtcccttt gattcaaagc taaattcata tatcttatcg tctgtaccat
    18181 ataagattgc atctataaaa tctcttggta attcattcca aggtatatct aggtctatat
    18241 ttttatcttt tgctattgca tataattcac ctaccatcca attaccattt ggctttttcc
    18301 ccttcaattt tccaaaatag taagtagctc catctaatat tgaaatagtt gggtctttta
    18361 caatcaattg aggattaact tgataggtaa aacctaatcc attacaatcg tgacaagctc
    18421 cagtatggga gttagcattg aaggttgaag tatttatctt agaaaaaata gtttgacaat
    18481 gtggacaata attttttgta ttattctttt tttctaatgg aattttacaa tatggacagt
    18541 aactttttcc gattgataca aataacattc ttaaataatc ccaaatgctt gtaagagtac
    18601 caacagtaga gcgaggattt tggcttcctt ttttctgttc gatagctata gtaggggtta
    18661 atccctgaag taaatcaaat tccggttttt gaagctgttc tgaaagtttt gctccattac
    18721 ttagattttc aaaataacgt ctttttcctt cttcataaat cgtatcgaaa gctaaagaag
    18781 attttccact accacttaca cctgtaataa cagttatttg attttttgga attgtaacct
    18841 caacatcctt taaattgttt tgccttcctc cttttaaata gatagagctt ttattatgac
    18901 aaagtgaatt tttaattgat ttatttattg attttgtaaa tttgaatttc ttttcgagtt
    18961 caagaggatt gcctaattct tttatttccg aaatgcctaa tatttttaac tgagaaaaga
    19021 ttgatttcaa ataatcttca ggcaattttt ctattaacaa attaaggtct tctattttta
    19081 tatttccgct tgataatatt tctgccattt catctaataa atttttttct gctcttgtta
    19141 attgattgta ttttttggaa cttaattctg tccataaatc ccatggaagc aattctaaac
    19201 caatcatatt attaaagtca tgtagaagat taccctttac agaatgccaa cctttccaat
    19261 ttttattata tccaaagtca tcttgatgag catgtccttg ccgacatctt atccaggctt
    19321 ctgaaccagt taaaaaacca tctgtttttc cgacatcatg agtgtcaaaa ttaatttgaa
    19381 aataattttt ttgtatgtca tctatttgtg catctattct tatccatctc tttgttagag
    19441 tatcatagta ttctacaagc cagtggtctt cataagtaag ctcactttca aaataattag
    19501 caaaacctgc tctcattcta gcctcaaaac ctcgatatct taggagcgat acaagaagta
    19561 aagaataatc tctgcacatt ccaacaagcc tttgttttgg gtttcttgga atagttagtg
    19621 ggttagaatt tatttcaaat attcttttta acatttgagg aattgtacga agcaattcct
    19681 catcagattg ttgtttatta aaggatatac catataattt tccttggtct ccatgaatta
    19741 ctaggctttg aaccactttt aataactcaa ttggagtctt tggtaaatct tgatatttat
    19801 tttcaaattt tttaggattt gtgatgatgc tttgattttt atagttattt attttcattt
    19861 atatttcacc tctctaaatg gatgatatac cctgacatgg tgtcatatgc aagggcaatt
    19921 taaatttttt ggccaaaatt taaaaataaa taaaaaatga tacagtgata aattgtaata
    19981 caaatatagt ttcaaattgc atataatata attatttaat aaaaccatta tctatggaat
    20041 gttaatgaaa tgttaagatg gcattcttga atattaagaa atagaatact ataatatagt
    20101 gtgaaggatt tttagtaaaa aaatttgtaa gtgttaatat acattaaaaa atagatttat
    20161 aatttggagg aaaaaaatga aattatcaag gtataagaaa aatagtttag ttctgtctat
    20221 aattttatgt tcacttatta tttcaatcct tgtaattact gtttttaata ccgtaaaact
    20281 acacaatata tctaataaac aatctaaaaa ctatttaaat gatgtatcta cacagattgt
    20341 aatgaatgtt gattctaaaa tcaaatttat tttatccgac ttaagaataa tggcagattt
    20401 tattaagcaa tatgaaggtg atagtcgata tgagtattta tctaaaagaa agactagtta
    20461 taagtattat gatgttggaa taatagattt agaaggtaaa gctgagtttt taagtggaaa
    20521 aaagtttgac ttaaagaata ctgtttccta taaaaaagga attgaaggta aagaatactc
    20581 agaagtaata gagtcaatgg gttttgttct ttatagtgta cctatattta atgataatca
    20641 tgaagtatct agtattttaa taggtgtatc caataaagaa agtatgaatg agattcttaa
    20701 tatagataac tttaatggaa aaggaactat tgaaattatt aatagtaagg gaaaacctct
    20761 tttcatagga aaaaatactg aattaataaa ggaccttagc cataaatata agagttctgg
    20821 agatgaacct tgggcacaaa agatgcttga ggattttaaa aatgataaat cagggaatat
    20881 tacaataacc tcatcaaaag gtgtacaatg cttattaact tatcatccaa ttacaagagg
    20941 tttaaatgat tggcattttc ttctcatagt acctgaagat gctgtacttg gtgaacttaa
    21001 taaattaaac tcttttacaa taacaatgac atttataatt acatgtatta ttggaattat
    21061 aacattaatt ttatatatta taagaagaaa atatgtaaat caaatagaaa atattgcata
    21121 tttagatagt ataacaaatg gtataaattc aactaagttt agtatgctag tcaaaccttt
    21181 aatttcagaa tcacctgata gtacttatat gatgatagca atgaatataa aagattttaa
    21241 gctaataaat gattgtttcg gtagtgaaaa gggaaatatg acactaaaac atctttacaa
    21301 tattttgtat aaaaacattg atgaaaatga agagtttgtg tgccgtcatg atgcagacct
    21361 tttctatttg cttattaaga atagaccagt gttagaagcc ctggagttat tatataaaat
    21421 agaagctgac acaaattatt ttaataaaaa tagggaaaat ccatactttt taagactatc
    21481 agtaggtatt tacacaattg aaaatcataa tgaagattta attacaatac aagaccatgc
    21541 aaatacagca agaaaaagct ttaataaaac tcatcaaagt gatttttctt tttatagtga
    21601 tatagaaaga aaacgattaa tatctgaaaa ggaaatttca aatttaatgg aaaaagccct
    21661 tgaaaataaa gaatttttta tgtgcttaca acctaaaatt gatataaaaa gtggaaaaat
    21721 tagtggagca gagtctcttg tgcgttggaa aaattcagaa aagggcataa tttatccaag
    21781 tgattttatt cctcttttcg aaaaaagtgg atttatatgt aagttagatt tatatatact
    21841 agaagaaaca tgcaaattga tttctaagtg gataaaagaa ggtaaagaag tgttgagaat
    21901 ttctgttaat gtatcacgtc aacatctgaa tgataaattg tttttagaaa aatataagca
    21961 tatatgcaat aaatacaatg taccaacatg tctgatagat ttagaactta ctgaatctat
    22021 tttcttagaa aatccagaag ctatagatat aatagaggat attcattcta atggatttaa
    22081 atgttctata gacgattttg gatttgggta ctcatcatta gggatattaa aggattttaa
    22141 agttgatata attaagttag accgttcatt ctttgttagc aaaaataata tagaccgtgg
    22201 taaggtagtt attaagtcta ttattgaact tagcaaaagg ctaggtatga aagtaactgc
    22261 tgaaggaatt gaagaattag aacaagttga gttcctaaaa aatgtgggat gtgattatat
    22321 tcaaggttat gttttttcaa aaccattatt aatttctgac tttgaaaatt ttgcatatga
    22381 aaataataaa attaaaaata tccattatta aatgtacaag ataaaaaatt taggcaagtt
    22441 atataattaa aaatataact tgcctttttt atttatatat ttaataaaaa tatatttgca
    22501 gattttgtaa aaataatttt caaatgttgt aagttgtggc taaagcttta ttgtattatt
    22561 ctgaatatag aaagatagtt actgtcttag ataaaaactg aaacatacat aataatataa
    22621 aatttgctat attaataact ctaattattt tggaaggagg gaaaatatga taagtgggag
    22681 aatgttactc atcatgaatt ttctaaaagg aaaaaataag acaagttaca aggatattag
    22741 caaacaactt agtatagaag aaagaaaagt tagatatgat ataaataatt taaatattgt
    22801 tttgcgttca ttaaacaagt cagtaataca aaagatgaac aaaggtgtgt taattattcc
    22861 tgatgacttc caagtagtat gtataaataa taacgaatat gtattttcct taactgagcg
    22921 agtatctatt atgaaaatta tatcaatgtt taaaatagat aatctaaact tagaaaaatt
    22981 aagtaaagaa tttcaggttt ctagaagttc aattaaaaat gaccttaatg ttttgtcaag
    23041 tgaattagaa agaaaccaaa taactattac ttatgataaa tcttttaaaa taagtggaga
    23101 agaagacatt atatataagc aaagattgaa catattaaaa tcgtattctt atctttttgg
    23161 taaggaaaaa tctgatttaa gtgtatttga gaagtattta gtgaatgtaa ttgaaaaaat
    23221 attcaaaggg atttatttga ttgatatata taattggtct atgaatttat tattgcaaat
    23281 gggatggact ttaaatgatg aatcttttac atggtatgtg tcaaacattt tcctttttac
    23341 atggtatata cattcagatg aaaaacatcc tttagaatct gcttcattaa gtgagccgtg
    23401 ttttgaaaat aagtttgacc aagaatttga aacaattatt caaaaaaagt tgactaagga
    23461 acaaattttt ctgttaaaga gttttgtatt ttttacaaat aaatatgcaa gtttaaacga
    23521 ggatatggat ttgattagta cagaaattat agttcaagac ctaatttaca atatgtcaga
    23581 aatattagag attagcttta aagaagatat gattctttat aaaggattac tcaatcatat
    23641 tgccccattg atagagagaa tcaagcagaa tatacagatt tatgaggaat tacttcatgt
    23701 aataccagat aaatatcaat atgtacttga agctactaag gtttctattc aaaaaaacag
    23761 agttttaagt caaataaaga atgaaaatga aacaatactt ttggcaatac atttcttagg
    23821 aagtatacat agaaatgaga ggaataacta caaaaatata ttgttagttt gtggatttgg
    23881 atatggtgta attgctatgt taaaggatag tttagtaaat gattaccaga taaatatagt
    23941 agagagtatt ccatcatata agttacaaga ttataaagac tgggaaagta ttgacttcat
    24001 tataacaaca tcaaagatag taatagactt gcctaaaaca attattgaga taaatccatt
    24061 tttacaagaa gaggattatt tgaagtttga agaaaaaggg ataaaaagaa agaatgtctt
    24121 aactaattat atgtcaataa aaaaaaggtt ggacttttta gaagaagatg cccaaaaaag
    24181 agtattaagt attatatgtc aagaacttgg atacagtgat gagagaatat tatttagaca
    24241 acttaattta agtgatttaa tagggattga taccataagg ataattgatg gagatatgaa
    24301 atgggaagat gctgtaaaat ttagttcaaa catacttgct aattgtgatt ttgtatcagc
    24361 acaatatgct gagaatatta tagatatttt aaaaaatgtg ggtttttatg ctgtaaaaga
    24421 taacgaattt gctttactac atggcaatga tagttcatta gtcaaagtaa gttcgataag
    24481 tttattaata tgtaaagaag cagttagttt tggagataaa aaggttaaaa tcatattttg
    24541 cctagcaagc agagataaaa aagagcaaat tcctgcaata attaatctaa ctaaaatggt
    24601 ttataagact aattttattg ctatgttaga aagtgcaaag acaaaagaag aagcagaagt
    24661 tttaattcat aaatatgaga aggaggtcac tgtatgaata aattagttaa tgtaaaatgt
    24721 tcatttaaaa tacatgttaa ttcaaaagaa gaagccatta tatcgttagt tgatgtagtg
    24781 tcaaaagaag gatatttaat agataaaaat caatttttaa aagatgtttt aaagagagaa
    24841 gaaacattgt cgacttatat tggacatggt ataggtcttc ctcatagtca aagtgtaggt
    24901 gttaaaaact cttgtattac aataggaaag cttgatactc ctattgaatg gacagaagaa
    24961 ggagaaaaag tggatttaat atttttgata tcagtaacaa aagacaatga aaataatctt
    25021 catttaaaga ttttatcaaa attagcacgt ttattgatgc atgaaagttt tagaaatcaa
    25081 attagatgtt cagatgaaca aactgtttat aatcttataa aagaaaaaat agaggaggaa
    25141 gattaaaatg agaattttaa agtttttgca aaaacatctt atgacagcaa catcttatat
    25201 gattccattt gtggttgcag gaggaatatt atttgcttta tcagtaactt taagtggaca
    25261 agctgctgtt cctgaaacag gctggttggc taaactgaat caaattggag ctgctggctt
    25321 ggctttattc attcctatat taggtggata tatagcattt agtatggcag ataaaccagg
    25381 attagctcca ggtatgatag gtgcatattt agcaaaagaa gtaaatgcag gttttatagg
    25441 tggtatcata gcaggattta tagcaggatt tgttgtttta caattaaaga aaattaaatt
    25501 agctcctaca atgagaactt taggttctat atttatttat cctttagttg gtacattgat
    25561 tacaggtggt ataattgtat ttttaattgg agaacctata gcttctttta tgacatggat
    25621 gacaaactgg ttaaatggaa tgtctggtgt ttctaaaatt cctttaggtg gaatcttagg
    25681 aggaatgata gcctcagata tgggtggccc tataaataag gttgctgcta cttttgccca
    25741 aactcaagtt gacacacttc catatttaat gggtggagtt ggcgttgcaa tttgtatacc
    25801 tccaattggt ttaggattag ctacattatt gtttccaaaa aaatttagca aggaagaaag
    25861 agattcaggt aaagcttcac tattaatggg atgtgttgga attacagaag gagctattcc
    25921 atttgcaaca tcagatccag ttagagtaat accttgtatt atggctgggt cgattgtagg
    25981 aaatattatg gcttttttat taggatgttt aaatcatgca ccctggggag gtctgatagt
    26041 gttacctgta gttgacaata ggttggggta tattgcatca gtattaactg gagcagtagt
    26101 tgttgcggta ttaatgaaat tagtaaaaaa agacgtgaaa gaagatgaag aaattgaaga
    26161 agatttagat gaatcaattg aattaatatt tgaagaatta taaatataaa tggaaggaat
    26221 ttgtataaaa aagtaaatat aaaactataa atttaaagga gataagaata tgaaaattgt
    26281 agcagtaaca tcttgtccta gtggagtagc acatacttac atgtcggctg aatcacttga
    26341 attatcagcc aaaaaatttg gtatagaaat aaaggttgag actcaaggaa gctctggaat
    26401 agataatgaa ttgtcactta aagatattga tgaagcaact tgtgtaatat tgactaatga
    26461 tgttgagatt agaaatatgg aacgttttaa aggaaagaag gtagtaagaa tgagtgtatc
    26521 tgagataatt aagaaatctg atgcattaat taagaaaata aaagatactt ttcaataaaa
    26581 attgatttat tttacatata actcaaagat gtttttaaag aaatttttat taaaagcatc
    26641 tttgaaaata tcttaaaaat agaataaaag gagatatagt catgaaaaag ttaccaataa
    26701 agactgtcgt gcaaagatta ctaaacttac aagaagaggg gaaaagtgca actcttttag
    26761 gaattggtcc tatgtcacca aacttgttac aagcaagttt tgaactagca aaagatgatg
    26821 attttccttt aatgtttatc gcaagtagaa atcaagtaga tgcagatgaa ctaggtggcg
    26881 gttatgtaaa tggttggaat caggaaactt ttacaaaaga tattaagaag gttgccgaca
    26941 aagttggatt tgatggttta tattatttat gtagagacca tggtgggcca tggcaaagag
    27001 ataaagagag aaatgaccat ttgcctgttg atgaagcaat ggagttagga aaaaaatctt
    27061 atttagcgga cattgaagca ggttttgacc tattgatgat tgaccctaca aaagacccat
    27121 ttgaaattgg aaaagtaatt ccactagatg tagtactaga aagaactgta gaattaattg
    27181 agtattgtga aaacgagaga aaaagactta atctaccaga cataggttat gaggttggta
    27241 cagaggaaac caatggaggt ctgacttcaa ctgagacgta tgaaacattt ataactagac
    27301 taaaggttga actcgataat agaggtttgc caatgccaac atttatagtt ggtcaaactg
    27361 gtactttaac tagaaagaca gaacaagtag gaacttttaa ttttagaaat gcttatgatt
    27421 tagctcaaat ggctaagaaa tatggagttg gtttaaaaga gcataatggc gattacttag
    27481 atgatgtaac tttattagaa catataccat ctcaaattat tgctacaaat gtagctcctc
    27541 aatatggtac tgaagaaact agggcatatt taaaattagc agaagttgag tgtaaattag
    27601 aaaaagaggg cttagttgag aaaacttcta atatcagaca tgttcttctt gttaatgcaa
    27661 ttgaatgtgg acgttggaga aaatgggtag taggagaaca aaaaaatcta actactgaag
    27721 agatatttaa ggatgaagtt ttatctaatg aaatactaga tattgcagga cattatacat
    27781 ttaataatga tgatgttaaa aaagaaatag aagtgcttta tgataattta agtaagaata
    27841 atattgatgg tcaaagattt gttgtagacc atattaaacg tcctttgcgt gattatgcag
    27901 aatgctataa cttaaaaggt gttactacga ggattttaaa taaataatag gacttatagt
    27961 ggtgagtatt gttatactag gtatttatag ttataattat acttaccact attaaattta
    28021 ggaggactaa tattttgatt aatttaattt ttgatgtaga tgatacttta tataatcaat
    28081 taacgccttt ttacactgca tataataaag tgttttcttc aataaaggat atttctattg
    28141 aagatttata tatgagtagt agaaagtata gtgatgaggt atttcatatg actgaaaatg
    28201 gagaaatgcc aatcaaagaa atgcatattt ataggataat gaaagctttt gaggagttgg
    28261 gaaattctat tacagaaaaa gatgcacaaa gttttcaaga tgagtatata tatcaacaat
    28321 cacaaattac actaatacca gaagttgaga ggattttaaa tttttctaaa gaaagaaata
    28381 ttaatctagg aattataact aatggcccat caaaccatca aagaatgaag ttaaagcagt
    28441 taaatattga aaactgggtt gataaaagta atatttttat ttctagtgag gttggatttt
    28501 ctaagccaga tactaatatt tttagagtag ctgagaatgt tatgaattta gatagagaaa
    28561 atacatatta tgtgggtgat tcttatagaa atgatgtatt aggtgctaaa aaagctggtt
    28621 ggaagagtat atggttgaac catagaggac atgaggtaga agagttgttt tacaaacctg
    28681 attttgtaat tttggaacat aaggatttga tatctttatt tataaaaata tgtagttata
    28741 aaaatatgta gtaaaaaaat gttaataata gaattgtata aaataattaa taataactat
    28801 ataaaattat atgtttagat tgtgagcaat ctattttatg ttaaaaatag attatttaga
    28861 agaaggggaa atcttaaaaa aatttgaata tccccttttt attatttaat taaaatgaaa
    28921 tagttagaaa tatattttat ttagtttagt caataacatt ttgggattct ttataactac
    28981 atgttaactg ttgaataggt ttatcatatg gtttaaccca tcctttatta atacttaatt
    29041 ctgtaagtgc tgtgtgacct tctaccattt gagttaatga agctgaatat atagctctta
    29101 attcaggtgt actacttgtc aatgcagaat taagatacat attggcagat gctttagcag
    29161 atgaaagcat acttaacatt attactttat cgtctatata agtattgttc ttaattagat
    29221 tttctatcat atttctcatg ttatctatac ctcctcagaa attagtattt cattttcaac
    29281 tataaattgt tgcatagctt taattcttcc ttctgttgct aatatactag attctgattg
    29341 tctttttagg tcttcatctg atattaacat atttattgct ctttgcataa gtaatccgtc
    29401 agactccatt tttataactg ctgctaaaga tattttttca gtttctgaaa gtattctcat
    29461 ttaatcatct cctatattgt aatttatgct ttaatagtat gtctttcttt tcaaggaaat
    29521 aaccattaca cttttttaca gtattttata ttctctttat atcaaaattt agtcgaccta
    29581 atatattaat aataattttg taggatattt atcattaaat atctataaaa taaattattt
    29641 gaatattcat aaagcaaaat ttatttgtat gtacttcttc aagagtcaat atgagttttt
    29701 tcaaaaaatt aatttttatg ataatcatta agggctatat ttttcttaaa gttgtgatta
    29761 tcattattaa tttacaataa aattgccgaa aagccctctc ctttaggtgt agggatgaaa
    29821 ggtaaggtct taatataaaa aaagttagaa taatgcttgt aatctaatac cgctactgat
    29881 accctatatt taggaggtgt tagtttttat gtataaatct aacaaaaata tagtttatga
    29941 ttgcaaatat catattgtat tttgccctaa gtatcgtaaa cctgttttag ttggcgaagt
    30001 tgaaaagatg ttaaaagaaa tactaccata taaagcagat gaacttggag cagaaataat
    30061 agaaatggaa actgataaag aacatgtgca cttattaata tcttgtgacc ctcaatatgg
    30121 aattcataaa gtagtaaaag gtttaaaagg ctttagttcg agagtattaa gagagaactt
    30181 tccacattta aagtctagta tgcctagcat gtggactaat agctattttg taggtactgt
    30241 tggttctgtg agtttagaag tagttaaaca atatatagaa aatcaacaag ttaggacaaa
    30301 gaaatgataa aaactcatag agtgaaatta aacctaacta gaacacaatt tgaattagta
    30361 agggaaaagc aaatggaaag tgctaactgt tggaactaca tagttaactt atctaaagaa
    30421 tactattttg aacataaaca atggattaaa aagaatgata tacaaaagtt aatcaaaggt
    30481 aagtacaatc ttcatagtca aactattcaa gctatatcag ataaatttga tgcaaataga
    30541 aagactatat cagaattaag aaaaaaaggt aatactaaag caaaatatcc atacaagact
    30601 aagaaatttt atataatacc ttttaaagct agtgctatta atagaaatgc taaaggcaac
    30661 ttaaaattat caatgtcaaa aggcaggtat ttagaattag attttaatgt tgagaatata
    30721 aaaactgctg aaatagtatg gcgaaatgga tattatttat actacacttt tgacaatgaa
    30781 ttaaatggag ttgtagccaa aggcactaat accgtaggtg tagacttagg ggaaatacat
    30841 tcgatagcta gtgtaacaaa tgaaggtgta gggcttattc tatctaatag agaaggcaga
    30901 agtattaaac aatttagaaa taagatgtat gcttatatat ctaaaagatt aagtaaatgt
    30961 aaaaaaggta gtagacaatc aaagaagtta tggagattaa aaaataagat aagaagtaaa
    31021 acagataatc agttaatgaa tttatatcat caaacaacta gaaagtttat agacttttgt
    31081 gtagagcaga aggtatgtga aatagttctt ggggatataa aaggggttga aaaagatact
    31141 aagaaaaaga aaagattaaa cagagttaat agacagaaaa tatctcaaat ggagtatggt
    31201 cgaataaagg actatataaa atataaagca aaagagcaag gtattgaagt taagttagtt
    31261 aaagaaaact acacttctca aacttgccca aagtgttcta aaaaacacaa accaactggt
    31321 agaacttata gttgtacttg tggttatgaa actcatagag atatagttgg tgcttggaat
    31381 attttaaata aaaagcataa atatggttta gtagatttta gaataaatca taagcaacca
    31441 ataaatttaa aagtatctac cgtttgagcc atgtgtgggc gaataacttt aaggtagtag
    31501 tggcgaagac accgtccgaa gtagcttggc tttgtaacca cctctgttgt actgtttaag
    31561 tataaataag tgtgttagcc accaagaatc cccttgcttt agctatgggg agtgtgtcaa
    31621 taaaatactt taatgaaaaa tagctaactt taattgtagg atattcttaa tatatttgaa
    31681 ataaataaaa atattgaagg atataatatt caattggtat ataaataata aatgatagtt
    31741 aaattttacc ttagaggcta attaggtgtt tagtcaaagc taatgccttt agaatacaaa
    31801 tgtattctaa aggcattttt actatgtatt ttcatttata ttactaaaca ttatctattt
    31861 ttatatacat ttctatgtat tttattttca tcaaatcggt ctataaactc atttttttct
    31921 tcactatatc caatagatat aagtgcataa ggaactaatt ttaatggtaa gtctaaagtt
    31981 tgtctaactt tatttaccac tttattttta ggaaatactc ctgcccaaca agcagctaga
    32041 ccttgattag taacttctaa taataaattt tgtgtgcaag cacctaaatc ttgtatccat
    32101 ttaccaggtt ttaagaattt atctctattt ccaagcacta ctatacataa tggtgcaaat
    32161 tctatatgct ttgctctatg ctgtgacttt gagagctgtt tcagcagttc cttatcatct
    32221 actataataa attcccaagg ttgagcattt tttgatgaag gtgcttgcat agctgatttt
    32281 agtatttcag ttattttatc ttcttctata tttttatcta tatatgttct tattgaacgt
    32341 ctatttttta taacttctaa catacagatt ctcctttaaa taaaattatt tctatttata
    32401 taatatcatt actttaaatt ttagctatta cttatataaa gaataaactt tgtgttggtt
    32461 ttatctaagt aaaagtaaca ttcagaacta aaaaacaaga ctataaatcc aaagtattaa
    32521 ttaaaaaaga agtaataaaa aataatatac aagatttaaa aaagagtatt gggcttaaaa
    32581 tttaattaga aaaccaattt tgagataatc tgttttttat tttaacaaaa aaacacaaat
    32641 aaagttcgat acgtagcgaa tgaaaaaatt acaagaaata ttttttttaa aaaatttcaa
    32701 aaaagagttg ataatatatg tatattaata taaaatttaa ggtgtataat tcgtataata
    32761 atacgaatga tttgtagtgt aaaataactt ataaacggag aggggaagtc taatgaaagt
    32821 agcagtagtt atgggttcaa aatcagatta tccaaaatta gaagaaggga taaaactgct
    32881 tgaaaaatat ggaatagaag ttgtagcaag agcattatca gctcatagaa caccagaaca
    32941 actttcaata ttcctaaaag aaatagaaga tgatactgat gtaataatag cagcagcagg
    33001 aaaagcagca cacttaccag gtgtaatagc ttcacaaaca ttaattcctg taataggatt
    33061 acctattaaa tcatcaacta tggatggact agactcactt ttatcaatag ttcaaatgcc
    33121 aaaaggaatc ccagttgcaa cagttacaat agacttaggt ttaaacgcag ctttattggc
    33181 attacaaata atgactttaa aatacccaaa attaaaagag gatttgaaat catatagaga
    33241 agagatggca caaaaggtgt tagaagacga caagaattta agggggtagc agaaatgtta
    33301 ttatatgaag gaaaagcaaa acaagtatat tctacagata atgaaaatga atatgttgtt
    33361 tattataaag atgatgcaac agcatttaat ggagaaaaaa aagcagaaat atcttcaaaa
    33421 ggtatactaa ataataagat atcaacaata atctttgaaa tgttaaaaga aaataatata
    33481 aatactcact ttataaaaag tttatcagat agagaaatgt tagttaaaaa agtagagatt
    33541 ttaccattag aagtaatagt aagaaatatt gctgctggtt ctatctgtaa aagagttggt
    33601 cttgaagaag gtgtagtttt cgatgagcca atttttgaaa taagctacaa aaatgatgct
    33661 tatggtgacc caatgctaaa tgatgattat gcagtggcaa tgaagttagc aactagagaa
    33721 gagttaaaat tcttaagaga agaaacttta aaaataaatg agttactaaa agcatttttc
    33781 ttaaaattaa acctaaagtt agttgacttt aaaatagaat ttggtaagga ttctgaagga
    33841 aatataatat tggcagatga agtatcacca gacacttgta gactttggga tgtaaataca
    33901 aatgaaaagt tagataagga cagatttaga aaagaccttg gagaccttgt agaaggttat
    33961 acggaagtac tttcaagaat gaataataaa taggaggcaa atgtatgtgt ggagttttag
    34021 ggatttactc taataaagat gtaactaaag aactatatta ttctttatat tctatgcaac
    34081 acagggggca agaaagctgt ggtttagctc ttttagatga tggagaaatt aagtacaaaa
    34141 aagatatggg attagttgga gatgttttta aggaaaatga attatctaag ttaaaaggaa
    34201 atatagggat tggacatgtt agatattcaa cagctggagg aagtcatgtt tctaactgtc
    34261 aacctttagt tggaagttgt agaaaaagac aattagccat agctcataat ggaaatctag
    34321 ttaatgcaaa ttatttaaaa gatatgctag aagaagatgg atatatgttc caaactaatt
    34381 cagatacaga agtgatttta tatatacttg ctagatatta caaaggggat atagtagaaa
    34441 gtttaaaagt gacaatggat tatataaaag gtgcatatgc tcttgtaata atgagtcaag
    34501 aagaattggt tgcagttaga gacccacatg gattcagacc tcttgtactt ggtaaaaagg
    34561 gtgatgaata tatatttgca tcagaaaatt gtgcaataga tatacttggc ggagaagtta
    34621 taagagatgt agagccagga gagataatag tagtcaaaga tggagaacta aaatcatatt
    34681 tttattcaga aaattataaa ccagttaaga aaagttgtat atttgaacac atatactttg
    34741 caagaaatga tgcaactata gacaatgtaa atgcatatga atttagaatt aaatgtggtg
    34801 agagattagc tcagaatgaa actgtaaagg cagatatggt agttccagtt ccagattcag
    34861 gttggccagg agctattggt tatgcaaatg ctagtggatt aaaaataagt gaaggcttag
    34921 ttaaaaatag atatgtagga agaacattta taaaacctac acaagaagaa agagaaatag
    34981 cagtaaaaat aaaattaaat cctctttcaa caataataaa aggaaaatct ataattttag
    35041 tagacgactc aattgtaaga ggaactacat ctaaacaatt agtaaaatct ttaagagaag
    35101 caggagcaaa agaaattcat cttagaataa cttctcctcc agtagcttac tcttgttact
    35161 atggaataga tactccaaat cgctctaaat taattgcatc aagtaacaac gtagaagaaa
    35221 tgagagaata tattggatgt gatagtttaa aattcctaga tatagaagga atgttagatg
    35281 caacagaaca taagtctact ttctgtaaag cttgttttga tggagaatac ccagttaaga
    35341 aaattgataa ggaggaacta ctttcatgtt aacatataaa gaatcaggtg tagatataga
    35401 tgaaggaaat agagcagtag accttataaa aggtaaaata aaaggaactt atgatggaaa
    35461 tgtagttgga gatttaggta acttcagtgg attatacagt ttaaaagatt ttgtaggtat
    35521 gaaagaacca gttttgcttg catctacaga tggtgttggg actaagttaa aaatagctca
    35581 gatgatggac aagcatgata ctgttggaat agatttagtt gcaatgtgcg tgaatgattt
    35641 aatttgtcaa ggagcaaaac cattattctt ccttgactat atagctcttg ggaaattagt
    35701 gcctgagcat atcgaaaaaa tagttggtgg aattgcagat ggttgtaaaa tgtctggttg
    35761 tgcactaatt ggtggagaaa ctgctgaaat gcctggaatg tatggagaag atgactatga
    35821 tttagcagga ttttctgtag gaatagcaga caaagaaaag attgtttctg gaaacaatgt
    35881 taaatcagga gatgtattgg ttggtatatc ttcaagtgga gtacacagca atggattttc
    35941 ttttataaga aagatatttt tagaaactta caattataaa atggaacaat atgttgaaga
    36001 actaggaatg acagttggag aagcactttt aacaccaact aaaatatatg ttaaattagc
    36061 tttagatgta ttagctaaac atgacataaa agctatagct cacataactg gtggagggct
    36121 tatagaaaat ataacaagag ttatacctaa agggttagga ctagatatca ataaaaaatc
    36181 ttgggagaag cctcctatat ttaaaatgat agaaggtttt aatgctgttg atgagagaga
    36241 acttcataag agttttaaca tgggtattgg gctagtttta atagtagata aagaaaatgc
    36301 tgatgatgtt gtaaacttta taaataatag agaaaatgac aatgctgctt atgtagataa
    36361 aaaatatagt gaattattag aagataaagc ttatataatt ggtgaagtag tagatagtca
    36421 tgaaggtgta gaattatgct aaatatagga gttttaatat ctggtggagg aacaaattta
    36481 caagcagtta tagatggtac tgagtctgga gaaataaaag gtcaggttaa agtagttatc
    36541 tcaagtaagc aaggtgctta tgggctagag agagctaaaa atcataacat aaaagcaatt
    36601 tgtgaaactg atgaagacaa gataatagaa attcttaagg aaaataaaat agatttagta
    36661 gttttggctg gttatttaaa aataatcagc ccaaagctgg ttaacgagtt tagaaacaaa
    36721 atgataaaca tacatccatc attaattcct tcattttgtg gagctggatt ctatggagaa
    36781 aaagttcatc aaggtgttat agattatgga gctaaagtaa ctggtgctac agttcatttt
    36841 gtagatgaag gtgctgatac tggaccaatc ataatgcaag atgtagttaa ggtaaatcaa
    36901 gatgatgatg caaagacact tgcaaaaaga gtgttagaag ttgaacatag gattttaaaa
    36961 gaaagtataa gtcttttttg tgaaaataaa ttaaaacttc aaggtaggag agtgtttata
    37021 aatgagtaag agagctttaa taagtgtaac tgacaagact ggtgtagtgg aatttgctaa
    37081 agagttaaat aaattagatt atgaaattat atctacagga aatacattta aaacattaaa
    37141 agaaaatggt gttaatgtaa tgcaagttga agatgttaca aattttccag aaatattaga
    37201 tggaagagta aaaacattaa atccatatat acatggtggt atactttata aaagagataa
    37261 agaatctcat gtagaaactg taaatgaaca caaaatacat tcaatagatt tagtagctgt
    37321 aaatctatat gattttgaag gaactttaaa agctggaaaa agtcatgatg aaataattga
    37381 aaatatagat attggtggac catctatgat acgttctgct gccaaaaact ataaggatgt
    37441 aatagtagta gtagatataa aagattatga ttctataata gaaaaattaa aaacagatac
    37501 aatgacatta gaagacagaa aaaaattatc atataaagct ttctcaacta caggaagata
    37561 tgatgcgtta atatctagtt attttgctgg agaagttgga gatacgtatc ctgacatatt
    37621 aaatttaact ttccaaaaag aacaaacttt aagatatgga gaaaatccac atcaaaatgg
    37681 tttcttatat tcacagtcaa atgcaaaaaa tccaatctta aattatgagc aattaggcgg
    37741 taaagaactt tcttttaaca acttaaatga tttacatgga tgtttagaag taatgagaga
    37801 atttaaagat agtgaagaag tagtttcagt agctataaaa catgctaatt catgtggggt
    37861 aggtttagga aaagatgctt ttgaagcata tacaaaatgt tatgaagcgg ataaggtttc
    37921 aatatttgga ggaatagtag gaataacttc tactatagat aaagctacag cagagaaatt
    37981 aaatgaaata ttcttagaaa tagtagtagc atatgatttt gaaccagaag ctttagagat
    38041 acttaaacaa aagaaaaatc taagaatatt aaaattagct aaaatagaaa atagtttaca
    38101 accatatgaa atgaaatatt tagatggaaa attattgata caagatagaa acaatatatt
    38161 agcagaaaaa agtgaaaatg taacaaagga aaaaccaaca gatgcacaac ttaaggacat
    38221 ggaatttggt atgagagtag taaaaaatat gaaatcaaat gcaattgcag tagtaaaaaa
    38281 tggacaaact ctagctttag gttgtggaca aacatcaaga atttgggcat taaaaaatgc
    38341 tcttgaaaat aacaaagata aagattttac aggagctgtt ttggcgtcag acgcattttt
    38401 cccatttgat gattgtgtaa cattagctca tgaatatgga atatcagcag tagttcaacc
    38461 aggtggctct ataaaagata aagattcaat agaagcttgt gacaagtatg atatggttat
    38521 ggtatttaca ggaataagac acttcaaaca ctaaggagga tattatgaag atattggttg
    38581 taggcggtgg cggaagagag catgctatat gctggaaact aagcaaagaa aaaaatgtag
    38641 aaaaaattta ttgtgcacca ggtaatgcag gaattgcaaa cgtagcagaa tgtgtaaata
    38701 taggtgatac taatatagaa gaacttttaa aatttgccaa agaaaatgaa ataggtctga
    38761 caatagtagg accagaagta ccactagtta tgggaatagt agatgagttt gaaaaagaag
    38821 gattaagagt atttggacct aataaaaaat gtgctcaact tgaaggaagt aaagcttttt
    38881 caaaagaatt tatgataaaa cataacatac ctactgcaaa atataaagaa tatacaaact
    38941 tagaagaagc tatatcagaa atagatagtt ttggatatcc agtagtaata aaagcggacg
    39001 gattagctgc tggaaaaggt gttgtaatac ctgaaaatag agaagatgct atagcaactt
    39061 taaaagaaat gatgtcagat aagaagtttg gagctgctgg agataaaata gtaattgaag
    39121 agtttttaaa aggaatagaa acttcaattc tagcatttgt agacaatgat acaatagttc
    39181 ctatggcaag tgcaaaagac cataaaaaag taaacaacta tgagcaaggt ccaaacactg
    39241 gaggaatggg aactttttca cctagtgaaa tatatacaga agaattagca aataaagtaa
    39301 aagagacagt attagaaaaa actttagaag gtttcaaaaa agatggactt aattttaaag
    39361 gtattttatt tgttggtctt atgataactg aagatggaga aaaagtactt gaatataatg
    39421 taagatttgg agaccctgag acacaatctg ttttatttag attagaaaca gatttacatg
    39481 agataatgga agctatacta gataataagt taaaagatat agaaataaac tatagtgatg
    39541 atgaagctgt atgtgttatg cttacatcag gagggtatcc agacagttat gaaaaaggga
    39601 aaattataac tggtcttgaa aacttggatg atgatatagt agtattccat agtggcacta
    39661 agatgtttga tggtaatttg gttaccaatg gaggaagagt aattggtata actgcaaagt
    39721 caactagagt taaggatgca gcagaaaaag tttatgaaaa cataaagaag ataaactttg
    39781 aaggaatgca ctatagaaca gacataggaa gataattttt ctaaaatgga ggataaggta
    39841 tgttgaatac tgaaaataaa gactcaatgg ttagaagagt attggttgaa aaaagagaag
    39901 gttttgacct tgaggctaaa gctttaaaaa aagacttagt agaaagttta cacatagata
    39961 atatagaaaa tttaagaata ttaaatagat atgatgtaga aggaatatca gaagaagttt
    40021 atgaaaatgc agcaaagaca atattctctg aaccaaattt agatgtagta tattatgaag
    40081 aaataccaaa attaaatgat gaaagagtat ttgcaataga atttcttcct ggtcaatacg
    40141 accaaagagg tgattgggca gctcaatgtg tacaaattgt aaatcaaggg ataagacctg
    40201 ctattaatac agcaaaagtt tatatattat caggcaaaat aactgatgaa gagttttcta
    40261 aaataaaaga ttactgtata aatccagtag atagtagaga agcatcttta gagaaaccag
    40321 aaacgttaaa aatggaaact gaaataccaa caacagttga agtattagat ggatttatag
    40381 atttagatga aaatggatta agaacatttg taagtgaaaa aggattagca atgactttag
    40441 gagacttaca acacgttcaa aaatacttta aagatacaga aaagagaaat ccaactataa
    40501 cagaaataaa agtattagat acttattggt ctgatcattg tagacatact acttttatga
    40561 ctgaaataga aaatgtaaag atagaagatg gtaagtttaa tgatatagta aaagaagcgt
    40621 atcaaatgta tcttaactca agagacaatg tatatgtgaa cagacataaa gatatatgtt
    40681 taatggatat agccactgtt gcagtaaaag aattaaagaa aaatgggaaa ttaaatgacc
    40741 tagatgaaag tgaagaaata aatgcatgta gtataaacgt agatgttgaa gttgatggta
    40801 agatggaaaa atatctagta atgtttaaaa atgaaactca caaccatcca actgaaatag
    40861 aaccatttgg tggagcagct acttgtttag gaggagctat aagagaccca ttatcaggaa
    40921 gaagttatgt ttatcaagca atgcgtgtta caggaagtgc tgacccaaga acgactttag
    40981 aagatacttt acctggaaaa cttatgcaaa gaaaaataac tacagaagct gctcatggat
    41041 atagttcata tggtaaccaa ataggtctta caactggaca agttgcagaa gtatatgatg
    41101 aaaactttgt tgcaaagaga atggaaatag gtgcagttat agcagcagca ccaaaagaga
    41161 atgtagtaag agaaagacct gaagcaggag atgtaatcgt tcttcttgga ggaaaaacag
    41221 gaagagatgg ctgtggagga gcaactggtt catctaaaga acatagtgaa gagtcaatct
    41281 taacttgtag tgcagaagtt caaaaaggag atgctcctaa cgaaagaaaa atacaaagat
    41341 tctttagaaa taaagaagtt gctcaaatga taaaaagatg taatgatttt ggagcaggtg
    41401 gagtttgtgt tgctatagga gagattgctg atagcttaga tataaactta gacttagttc
    41461 ctaaaaaata tgatggactt gatggaactg agcttgcaat ttcagagtca caagagcgta
    41521 tggcagtagc cataaaaaaa gaaaataaag ataaatttat acaattagca gtagaagaaa
    41581 acttagaagc tacgcatgta gctacagtaa cagatacagg atatttaaga atgttctgga
    41641 atggaaaagc aattgtagat ataaatagag agtttttaga tacaaacgga gtaaaacaaa
    41701 ctactgatgt tcatgttact aaagtagatg aagaaaatac attctttagc tctaatgaaa
    41761 tagtaaaaga tgttaagtgt gcaagtatga aagacaagtt tacaaaagta ctatctgacc
    41821 ttaatgtttg ttctcaaaaa ggattagtag agatgtttga taatacaatt ggtggtaata
    41881 cagtacttat gccatttgga ggaaagtatc aagcgactcc aacacaaggt atggttgcta
    41941 agataccagt acttggagga gaaactaata cttctacaat aatgacttat ggatacaatc
    42001 caaaggtagg aaaatggagc ccattccatg gtgccttgta tgcagtagta gaatctgtgt
    42061 gtaaattggt tgctattgga ggaaattata gtacaacaag acttacattc caagagtatt
    42121 ttgaaaaact tggtaacaat ccagaaaaat ggggtaaacc attctcagca cttttaggag
    42181 cgttttatgc acaaagtaaa tttgagatac ctgctatagg tggaaaagac agtatgtctg
    42241 gtacatttaa agatattgaa gttccaccaa cattagtttc atttgctgtt gacacagtag
    42301 atgcgaagaa agtagtatct ccagaattta aaaaagcaga ctcaaaagta gtaatgttat
    42361 gtgtaaacaa ggcagaaaat gatgttgtag attttgagga attaaagaga aacttagata
    42421 aagtaagaga acttatacat ggaaataaag ttttatcaac atatgcatta ggatttgcag
    42481 gtgtaggtga agcaataagt aaaatggctt ttggtaataa aataggattt aagtttagtg
    42541 aagaagctga aaaagcattt acagataata aattatttga ggctagttat ggaaatatag
    42601 tacttgaatt agcaaatgat gatttaagta tgttagaagg atacaattat gttgtgattg
    42661 gaagtacagt taaagaagct agtatattta tcaaaggtga agaacttgca ttagatgaat
    42721 tatataaagc acattgtagt actttagagc ctatattccc aactaaaaca gaagaagtaa
    42781 aatcaaagat agaaactata agttatatat cacaaggaga agctaaaaaa tcatcattaa
    42841 gtatagctac tccaagagta tttataccag cattccctgg aacaaactgt gaatatgact
    42901 ctgcaagagc atttgaaaga gcaggagcta atgcaagtat aagagtattt aagaatttaa
    42961 cttataaaga catagaagat tctatagata caatagtaaa cgaaataaaa tcttctcaaa
    43021 taataatgtt accaggtgga tttagtgctg gtgatgagcc agatggttct ggtaaattta
    43081 tagctactgt atttagaaat cctagagtgc aagaagctat aaatgaattt ttaactcaaa
    43141 aagatggact aatgttaggt atctgtaatg gattccaagt attaattaaa ttagggttag
    43201 ttccatatgg agaaataaga gttccaagtg aaagtgctcc aacacttact tacaataata
    43261 ttggtcgcca tcaagctaag attgctagaa ctagaatttc atctaataaa tctccttggt
    43321 tagctcaaac taatgttgga gatattcata atatagcaat ttctcatgga gaaggtaagt
    43381 ttgttgccag tgaagatgtt atgagagaat taattgctaa tggacaagta gcaactcaat
    43441 atgttgactt taataatgaa gctacttatg atattgagtt taatccaaat ggttcattct
    43501 atgctgttga aggtataact agtgctgatg gtagagtgtt tggtaaaatg ggacattctg
    43561 aaagaattgg tgaggaagta tataagaata ttataggtga aaaagaccaa aagatatttg
    43621 aatctggagt taaatatttt agataaagct ttgatgttta attgatagct agaatatata
    43681 atttagatat ttagatttaa agagatgtaa taatatgatg ataatcattt aatattacat
    43741 ctctttttta tttatctaag aaataataag ttcaacctaa gttaaaatac aagctgtgtt
    43801 ataattaatt attaagtaaa attgaatatt tattggaggg caacataatt aatgaaaata
    43861 ttaataacag ggtctaatgg tcaattagga aaagaattgg ttaaccaatt agaagcaatc
    43921 aatcaaagta taaaccaacc aaaatatgtt atattagcta caacaagaag tgatttggac
    43981 atatccaatc agactaatgt agataatttt attttgcata ataaacctga tgtagttgta
    44041 aactgtgcag catatacaaa ggttgatgca tgtgaagata atatcgaaat tgcatacaaa
    44101 ataaatgctt taggagtgag aaatctagca atagcatcag aaaaagttaa tgctaagctt
    44161 attcacatat caactgacta tgtattcaat ggtttttcta aatatccata tagggaagac
    44221 aataaaactg aacccaattc tgtatatgga aaaagtaaac ttatgggaga aaaatttgtt
    44281 gaacaattct cacataagta ctttatattg agaacagcat ggttatatgg taatgggaat
    44341 aattttgtaa agaccatgat aaaactttct ttagaaaata aagaggttaa tgtggttaat
    44401 gaccagtttg gaactccaac aagtacagta gacttagcaa aaattataat aagaatcatg
    44461 gaaacagaac attatggagt ttatcatgct acatgtgaag gtgagtgtag ttggtatgat
    44521 tttgccaaaa agatatttaa actaaaaaat atagatataa aagtaaatcc aattaagagt
    44581 aacgaattta aatgtaaggc tccaagacca ctatattctg tattggataa ttttatgttg
    44641 aagttaattg gtttaaattc ttttagaaag tgggaagaat ccattgaaga atatttagaa
    44701 agatatgata taaatgaaaa gtaaatgtat tattttgaga aaagaaaata agatattata
    44761 aaaaatataa atatttcttt tatttatccg ataatcataa tatgacaaaa acaaatttaa
    44821 ggatggaaaa agatgaaagg aattatttta gcaggagggt ctggtacaag actatatcct
    44881 ataacaaaat gtatatcaaa acaaacactt ccaatatacg ataaaccaat gatatattac
    44941 cctatgtcgg ttcttatgtt ggcagggata caagatatat taataatatc tacaccaaga
    45001 gatataaaag catttgaaga attatttgga gatggaagtt cactagggct taacataaca
    45061 tatgcaattc aggagaaagc aaatggaatt gcagaagcgt ttataatagg agaagaattt
    45121 ataggaaatg acagagtgtc tcttgtatta ggggataata tattttatgg atatggattt
    45181 agtgaaaggt tggagaatgc agttaaaaga aaaggagcaa ctatatttgg gtatcatgta
    45241 agtaatccaa attcgtttgg agttgtagaa tttgataagg attttaatgt attgtcaata
    45301 gaagaaaaac ctacagttcc taaatctaat tatgctatac caggattata tttttatgat
    45361 aatgatgttg tagagatagc aaagctagtt gaaccgtcag atagaggtga attagaaata
    45421 acctctataa acagtgaata tctaaaacga ggaaaactaa aagtggaact tctaggaaga
    45481 ggtatggctt ggcttgatac tggtacacat aaaggtctat tggatgcttc taattatgtt
    45541 gaagtggttc aaacaagaca gggactgtat atagcatgtt tagaagagat tgcatataca
    45601 aaaggatata taagtagaga acaattaatg aaactagcca agccactgat aaaaactgaa
    45661 tatggtcaat atctattaaa attggctagt gaagatatgt aatatggtag ataaaattta
    45721 ttataaattt tattaaggga gtttattata atgggaaaaa tcaatgttat aaagactaaa
    45781 atagaaggat tatatatagt cgaaccaaat gtattttatg atgaaagagg atattttatg
    45841 gaaagctata ataaaaagga ttttatagag cagggtcttg atatgtgttt tattcaagat
    45901 aatgagtcta gctcaagaaa aggcgtttta agaggaatgc attttcaggt taaatatcct
    45961 caaggaaaat tagtcagagt tacagaggga gaagttttcg atgttgcagt tgatttaagg
    46021 aaagattcac aaacatttgg gaaatgggaa ggtgtaatat tgtctgataa gaataaaaaa
    46081 caattttata ttccagaagg gtttgcacat ggatttttag ttttatcaga aaaagcagta
    46141 tttagttaca aatgtacaaa ttattattac ccagaatttg agaatggctt tatatggaat
    46201 gacaagcaaa tatctattga gtggccttta aaaggaattg aagaaatatt tttatcagaa
    46261 aaggacaaaa aacaaaaaag ttttaaagat atttgtacaa gacgttaata aaaatttatt
    46321 agaattaaca tttaggagaa aaaatatgaa aaaaatactg ataactggag gagcaggatt
    46381 tattgggagt aattttatac attatatgct taacaagtat gtagattatt ttattgttaa
    46441 cctagacttg ttgacttatg ctggtaattt agagacatta atagaaatac aagacaattc
    46501 caattataaa tttgttaaag gagatatttc aaatagggag caggtgtata agctatttga
    46561 agaagaaagg tttgatatag ttgttaactt tgccgctgaa tctcatgtag ataggtcagt
    46621 tgagaatcct gatttattta ttaaaacaaa cattatagga acacaagtac ttttagatgc
    46681 atctataaaa tatggagtta aaaggtatca tcaaatatct acagacgaag tgtatggtga
    46741 tttaccaatt gatagaaagg atttattctt tacagaacaa tcttctatca atccaagttc
    46801 tccatattca gcttctaaag catcagcaga tttattggta agttcttatt atagaacata
    46861 tggattgtta acaactatat ctaggtgttc caacaattat gggccatatc attttcctga
    46921 aaaattgatt cctcttatga ttttaaatgc tttggaaaac aaacaattgc cagtatatgg
    46981 gaatggggaa aatgtaagag attggttaca tgtatatgac cattgtactg ctatagactt
    47041 aataatacat aaaggaaata taggggagat ttacaatata ggaggtcata atgaaagaag
    47101 taatctagaa gtagtaaaaa tgatattaaa tcttttaggt aaatctgaag agttaatatc
    47161 ctatgtaaat gatagacctg gtcatgattt aagatatgct attgatgcaa ctaaaataga
    47221 gaatgagtta ggttggaagg caaaatatga ctttgatttg ggtataaaag aaactgttaa
    47281 gtggtatata gaaaatgaat cttggtggaa agctgtttta tcagtataac tttaaaattt
    47341 agaaaggggc gttttatatt gaatgagata agttcactaa ttaatatatt agcattgact
    47401 caattatctg gtagtaatac tacaaatcaa tgtaattgta catataataa ttcatctgga
    47461 tttgatatga ttatgatgac cttgttaaaa gcattatcac aaaataatca acatacttct
    47521 aatagctaca atttattgtc taattctgag aatttattta atgaacttga taatgtagta
    47581 aataatacca ctagtagatt tatagatgtt gatacaaaag ataaaaatgt aaagttgaga
    47641 attgaaaatg ctgtagatca agcatcaaaa aaatataacg tagattcaaa tcttataaaa
    47701 gcaataatta aagttgaatc agattttaat ccaaataccg tttcttctgc tggtgctaag
    47761 ggtcttatgc aacttatgcc agaaaactgc agagacttag gagttacaga cccttttaat
    47821 atagaacaga atatagatgc tggaactaga catattaagg aatacattga tatgtttgga
    47881 gggagcatag aaatgggatt gatggcatat aatggtggtc ctggaagaat gaaaagtaga
    47941 ggagtagaat caatctctga cctgtataaa atgccaaaag aaacacaaaa ttatattcct
    48001 aaggttatga aatattatag agggtaggct ttattatggg aataaatata gataagctaa
    48061 acataagtcc aagtataaat atggaggtta aaaaactaaa agagagtaat aaattttctg
    48121 acaaattaaa ctctttaaaa caatcttctt cattggaaga tataaaacga aatttggaaa
    48181 gtgtaaaaaa ggcaggagaa aggcttgtac ttactcaaaa ctatggcgat attagtatat
    48241 ataaaaatgc agtaaaagaa tacctaaaat ctgttgtaga taatatgtat tcacttgata
    48301 aaaatagtag cttttgggag catcagtatt ataagaatgt aaagattgtt gatagtaaac
    48361 ttgaggatat gactagtaag ttaattagtg aagaaaaaga aaacatagat attgtttcta
    48421 ctgttgacat gatacaaggc ttattgatag atatgtatag ataaggttat acaaggagaa
    48481 aaattttatg aatagcaaaa aagaggatat atatgaattg aattttgggc aattaataga
    48541 agaaaaatct ggtgttatag aagaaaatca aaatgttttt aatgcaaaag taaatgtagc
    48601 tgtttctata ggaaatacaa cagaaacaat aaaaaatata ttagatttaa aagaaggctc
    48661 tataataaaa ttaagtaaga atgtggatga aaaattagat atatatgcaa acgatagatt
    48721 gtttgcttat ggagaaagca ttattacaaa tgagaggtta tctgtaagat tatctaaaat
    48781 agaagatcca gatgtaaatt agcattttaa atagtatccc tttaggctat tctgtatgag
    48841 tttagctaag gggatatttt tataaaaaat ttaaaatatg tgtgaattat ataaatattt
    48901 ttattttatg agcgataact aaattataag tatgtaattt tggaggtgaa actatggata
    48961 ttaaaaatgt aagttcaaat attgtagtga ataattattt aaagaataca tctggtataa
    49021 aaaaagtgga agaggttaaa aaaattaata aaacagataa gatagagtta tctaaatttg
    49081 caaaggaaat ggcaagtgtg tcaaatatga gtgatgagaa tattagaaat aaaaaagtcg
    49141 atgaaataaa aaatgcatta gaaaatggaa cttacaaaat aaaaccaaaa gaaattgcga
    49201 aaaaaatgat agaggatatg cgaggataaa gtcatgatta cagacttaaa agttataata
    49261 tatgaagaaa aaaatataat tagtaagatg atggaattat taaaagaaca atttgattat
    49321 atagttgaaa aagacttaga aaatttaaat atgttaaatc ctaaactaga agatattagc
    49381 agggagcttg catccataga aataaagaga agacaacttt ttggtgatga tatttcaatg
    49441 agtgaagttg ttgaaaatag taatgatgga tacttaaaag aaatatatac agacttgaaa
    49501 cacattcttg ctttagctaa aaatcaacaa gaatcaaatg attctctaat aaagaaagaa
    49561 cttattttta cgaaaaaaat gattaatttc ataaaaccag ctgataagca aactacaact
    49621 tataattctt atggaaatat tagaaaataa atattaggag gagaaatatg gcaggattat
    49681 tttccatttt aaatacagct aattcaggaa tgaatgcgca ccaaaaagca atacagacaa
    49741 tttctcataa tataagtaat ctagatacag acggttattc taggcaaagg accgagtttg
    49801 ccacaaatag tccaatgtat atgcctagtt tgagtaattc aattgcaaga ggacaattag
    49861 ggactggagt acatgtaacg gatgtaacaa gagctagaaa tagtttttat gattaccaat
    49921 ttagggcaga agctcataag tatggtaaga cagtttcaaa atatgattat tacaatacta
    49981 tagaaactat actaaatgaa ccatcagact atggaatatc tgcaggtata gatgattttt
    50041 ttaaaggatg ggagtcatta tcaaaggata taaatagtga cagtaaaaaa agtctagtgg
    50101 tagaaaacgc atctaattta gcaactttaa taagtgaaag ttataagaag cttgataagt
    50161 taaaagaaga tatagattct aatatagaca ctgagataaa acaaataaat gacatgttaa
    50221 aaagtcttga agatttaaat aaatctattg atattatatc tggttctggc tctacaccaa
    50281 atgatttact tgatgaaaga gatagaattt tagataactt gagttttaaa ctagactttg
    50341 aaaatagtga tgttaaaaat atgcttgcag atggtaaatt agaacttaat gaattaaaaa
    50401 atccagatgg aacttggaaa acaggtatat cagggacctt acaaggcttg tttgaaatgc
    50461 atggtaagat tgatacatat aaaagcgatt taaaagatgt atctgatgga ttagctaaac
    50521 agataaatga tgtttataat acgtctgcag gaataacagt aacagatttt tttataactt
    50581 caaatgtaga tggtgaagat attataaaga taaatccagc tataaagagt aacccaaatg
    50641 aattaaagct tactgctgat gaagcttcaa aaattgctaa gttaaaggat gaaaaaatag
    50701 atattggggt ggctggtgga aaagtaagta caataagtga ccactataaa gcctttgcag
    50761 aatcaatcgg tttagattct caaaaagtaa atcaagatga agcaaatcaa cgtaaaatta
    50821 taaataatat tgataactca agaatgagtg tttcaggtgt atctttggat gaagaaatga
    50881 cagagcttat aaaaatacaa cgttcatatc aagctagtgc aaaagttatg tcaacagctg
    50941 tgcaactact ggatgttgta ataaatggaa taatttaata gaagggggaa tttataatga
    51001 gagtcagtac aggtatgatg tctagcagct atctaaactc actacaagat aatctacaaa
    51061 gattggataa ggttaataga caagttaata caacaaaaga aataaacaag ctatcagata
    51121 atccatataa agcaataaag atattaaact caaaaagtga aataaagact atggagactt
    51181 atgttgagaa ttgtaaagat acagggaatt ggttagaaac tactgatact gcattagatc
    51241 agcttggaaa attattatca gacataaaga aaactttggt ggcaagtggt gatggtgcat
    51301 actcagatga tgaattaaag actcttagta atgaaactaa tgaaaaaatg aaagaaattg
    51361 caaatgcttt aaatgcaaca catgaaggga agtacatatt ttcaggttca aatacggggg
    51421 taccacctgt tgaatgtata gataatgctg atggttcagt gagtttaaag tttaattcta
    51481 gtctaaatct taataaatta aatgactctt tgagtatgga tatagctcaa ggaataagtg
    51541 tagattataa cgttaaattg agtagccttg gatttgaccc aacaaaaact gacccatttg
    51601 aagagttaaa taacatatca aaaaaattag caaatccaga tgctgatgct ataaaagagc
    51661 ttacaacaac ttgtttgggc gatatggaac aactaataga aaatacagta aatgttaggt
    51721 ctatatatgg gacaaaagca aatacagtag aggctatgaa ggaaaaaaac gatgatagtt
    51781 tgatacagtt aaaggatatt ttgtcacaaa atgaagaaat agattatggt gaaaaacttg
    51841 ttcaattaaa ggcagcagag ttaacttatc aagcttcact acaaacagga ggaaagttat
    51901 ttaatgtatc tatattagat tacatataat aaaataacgc agatatgatg aaggttacat
    51961 taaaaaaagg tatattaggt tttgaaaatt taaaagaata tgagctttta gatatagaag
    52021 ataatgatta tttaaaagaa tttaattcta ctgaagaaga aggtatagga tttgttgttg
    52081 tatctccatt tgagattatc aatgaatatg aaatagtttt aaatcaggaa acaatagaaa
    52141 aactagaagt gaaatctcca aaggatgttg tattatttaa tataattact cttggtcaga
    52201 catttgaaga atcaactgtg aacatgaaag ctcctatagt gataaatata aaaactaatt
    52261 ttggaatgca aataatttta caggatgata aatattcaat aaggcatcca ttattgagag
    52321 gtgatagtgg atgctagtaa tttcaagaaa aaaagatgaa gcagtactta taggagaaag
    52381 catagaagta aaaattgtag gggtagatgg aaacaatgtg aaactagcta tatcagcgcc
    52441 taacaatata agtatattaa gaaaagaaat atacgaaaaa gtaaagagtg agaatataaa
    52501 agctacaaat aaaaatataa aaattttaaa gtcattaaaa taataagctt ggaggaagta
    52561 atatatgtat ggagaaaatc cttataattc ttataaacaa aatgctgttt ttatggcatc
    52621 aaaggaacaa ttgttactta tgcttgttga tggagcagta aaatatacca aaatagccaa
    52681 aacagctatc ttagataaaa atacacaaaa agcgcataga gagcttataa gagttcagga
    52741 tatatttaca gaactaatgg ttacattaga ccaaaatgca ggtcaatggg caaaggatat
    52801 gtacagagtt tatgattttg taagatatga actttcaaga gctaatatta gaaaagacat
    52861 tcaaataata gataatgtgt tgcctgtaat agaacaaatt aaagatactt ggcatgaagc
    52921 tgataaaaaa agtaaagaag aaagaagtag gtacaaataa tggaattaaa tttagctgaa
    52981 ttagataagt tatcaaaaga ggaattacta ttgatgcttg tagatggaac agtaaaatat
    53041 accaatatat ccaaagaagc tcttttaaat aacgactatt tgaaagcaca taatgaactt
    53101 gtaagagttc aaaatatatt tacagaacta atgactacat tagaccaaga tgcaggtcaa
    53161 tgggcaaagg atatgtataa agtttatgaa tttataaaat ctgaattagc aatagctgat
    53221 gaaaataaag atattaaaat aattgatgat atattgccaa tagtaaaaca aattagagat
    53281 acatggcatg aagtttacaa tcaactaaaa atataattgg atggagggat aaaggtatgt
    53341 caagtataag tcctataaga gttacaggtc tttcaggaaa ttttgatatg gaaggcataa
    53401 tcgaagctag tatgattaga gacaaggaaa aagttgataa agcaaaacaa gaacaacaaa
    53461 tcgttaaatg gaagcaagaa atatatagaa atgttataca agaatcaaaa gatctttatg
    53521 ataaatatct aagcgtaaat tctcctaata gtatagtaag tgaaaaagca tactcttcta
    53581 caagaataac cagttctgat gaaagtatta tagtagcaaa aggctcagct ggtgcagaaa
    53641 aaataaatta tcaatttgca gtttctcaaa tggctgaacc agcaaaattt actattaaat
    53701 taaattcaag tgaacctatt gttcaacagt ttcctccaaa tgctagtgga gcgagttctt
    53761 taactatagg agatgtaaat ataccaatat ctgaacaaga tactacaagt actattgtaa
    53821 gtaagataaa ctccctttgc gcagataatg atataaaggc ttcttatagt gagatgacag
    53881 gtgaattgat tatttcaaga aaacagactg gttcgtcatc agacattaat ttaagagtaa
    53941 ttggaaatga caatttagct cagcaaattg ctaatgataa tggtatcaca tttataaatg
    54001 atgctggtgg aaacaaagtg gcaaatgtat atggaaagaa tctagaagct gatgtaactg
    54061 atgaacatgg aagagtaact catataagta aagaacaaaa ttcatttaat atagataata
    54121 ttgactataa tgtaaattca aaaggaactg caaagttgac ttctgtcact gatactgaag
    54181 aagctgttaa aaatatgcaa gcatttgtgg atgattataa taaactgatg gacaaggtct
    54241 atggtttagt tactactaaa aaaccaaaag attatccgcc tcttacagat gcccaaaaag
    54301 aagatatgac aactgaagaa atagaaaaat gggaaaagaa agctaaagaa ggtatactta
    54361 gaaatgatga tgagttaaga ggttttgttg aagatattca atctgcattt tttggagatg
    54421 gaaaaaatat tattgcatta agaaaactag gtatcaatga aagtgaaaat tacaataaaa
    54481 aaggtcaaat atcatttaat gcagatactt tttcaaaggc tcttatagat gatagtgata
    54541 aggtatacaa aacactagca ggttattctt cgaattatga tgataaggga atgtttgaaa
    54601 agctaaaaga tattgtatat gaatattctg gaagttcaac ttctaaactt cctaaaaaag
    54661 caggtataga aaaaactgct tctgctagtg aaaatgtata ttcaaaacaa attgcagagc
    54721 aagaaagaaa tataagcagg ttagttgaaa aaatgaatga taaagagaaa agactttatg
    54781 ctaaatattc agccttagaa tctttgttga atcagtattc ttcccaaatg aattatttct
    54841 cacaagcaca gggtaattaa ttatggataa attaaaagaa aaattaaatt tatataaaga
    54901 tatatcttta caaattataa atttgattga aaaggaagaa tatataaaga taagtattaa
    54961 gttaggtgaa agacaagaga ttataaatag tgtaagtgaa attgatagaa atgattttat
    55021 acagctttat aatagaatgg aattaattga aattgatagc aggataagag atattttaca
    55081 aggacaatta ttagaagtaa aaaaagaatt acatgagtat aagcttacta aacaagtgaa
    55141 cacaatgtat tataacttaa atagagaaaa agtaaatatt tttaataaaa aagtataaag
    55201 ttatgtacca tcttgtccga ttatataaat gaagaattaa taaaaaagat taaaagtaga
    55261 aatgacaagg atgtcaacta tactaaggag ggtaaaataa tgagagttaa tacaaatgta
    55321 agtgctttga tagcaaataa ccaaatggga agaaacgtaa atgcacaaag taagtctatg
    55381 gagaagctat cttctggtgt tagaattaag agagctgctg acgatgctgc tgggcttgct
    55441 atatctgaga aaatgagagc tcaaataaaa ggtttagacc aagcaggaag aaacgttcaa
    55501 gatggtatat ctgttgtaca aacagcagaa ggtgcattag aagaaactgg taacatacta
    55561 caaagaatga gaactttatc agtacaatct tcaaatgaaa ctaatactgc tgaagaaaga
    55621 caaaagatag ctgatgagtt actacaatta aaagatgaag ttgaaagaat atcaagctca
    55681 attgagttta atggtaagaa attacttgat ggaagttcta cagaaataag attacaagtt
    55741 ggagcaaact ttggaacaaa tgttgcagga acatctaaca acaacaatga aataaaagtt
    55801 gctctagtaa atacttcaag tataatgagt aaagcaggta taacaagttc tacaatagca
    55861 agcttaaatg cagatggaac ttctggaaca aatgcagcta aacaaatggt atctagctta
    55921 gatgtagctc ttaaagaatt aaatacatca agagcaaaat taggggcaca acaaaataga
    55981 ctagaatcaa ctcaaaacaa cttaaataat actatagaaa atgttacagc agctgaatca
    56041 agaataagag atacagatgt tgcttcagaa atggttaact tatctaaaat gaatatatta
    56101 gttcaagcat cacagtcaat gcttgctcaa gctaatcaac aaccacaagg agttttacaa
    56161 ttattaggat aaaataattt tgtatattgg atatagaaga aagggtaagg ctttgcttta
    56221 ctcctttctt tttttacaaa tatacttttg aaaggaaaat ttatatgtta cttagtatag
    56281 ttatgattgt taaaaatgaa gaaaaaatac ttgaaaaaac tttaaaatca ttaaccacat
    56341 taagaaattc tatagaaagt gaacttataa tagtcgatac aggttctaca gatgatacta
    56401 taaaaatatc taagaagtac acggaaaaag tgtattttca taattggaat aatgattttt
    56461 ctagtatgag aaatatatca atatcctatg ctaaaggtga atggttactt atattagatg
    56521 cagatgaaat actaattgat tcttcaacta ttgtaaaatt ttttaaagat ggtcttgata
    56581 aaaaatttaa ttctgcttca atacaattaa aaaatcttta ctcatatgat aaaaaattat
    56641 atggatattg ttcagtgctt agacttttta aaaacgttga ttttagatac catggtaaag
    56701 tacatgaaca accaatatac aaaaacccta tatttaataa tgttgctgat tttgaacatt
    56761 atggatattt gtttgaagat gaagaaataa gattaaataa ggtgaaaaga aatgaaaaac
    56821 tattatttca agagttaaaa gagaatgata attctccata tacaaactat caattaggga
    56881 aaaattttat tatattagga aaatatcagg aggctttata ctatcttgag aaatcttatg
    56941 aattatattc taaactagaa ttggcacctg gttacttgat aacaagcctt gtaaaaacat
    57001 atttatattt aggaaaacat aagaagtgtg aaagattgtg cttaaagtat attaagaaag
    57061 acatgaataa tattgatata tattattata ttgcacaagc acaagtagat ttaggaaagt
    57121 atgaaaatag tatagattcg tataagagat atatttactt gttagataat tatgaaataa
    57181 gtacacaagc aaatagttta ttttcagaca ctgatactgt agggcttaga gatgaggcta
    57241 ttattacact gataaagatt tattataagc ttgaaaaata tgagtttgta atttcagagt
    57301 ataataacat tgaagatgtg gaaaagaaaa agaatgtgta ctttagctta tttatgtcac
    57361 tatataaatt gaatagattt gaagatataa aaaattatta taaagaagtt tcaaattcta
    57421 aaattgaaaa aaatagtttt tatagaaata tagaagaagt tataaataat attaaagagg
    57481 atgaaaaaga gtatgtatat aaaatccttt gtgacattga aggtaattat ggcattttaa
    57541 atcaaataag gataaataaa tgtgtatcta tagataaatg taaagaaata ctcaatgaag
    57601 aaaaggaaat aatttatgca tatattataa aaatagcttt tgaagagaag attgatttac
    57661 tagatatttt ttatgaaatg gaatatgtat ggatagagaa atatctaaaa tatcttttag
    57721 cattagaaag aagttttaat ttagtactat ataagtacat aattagtcgt ccaaatacat
    57781 cagatatgga aaagataaga gtatataaag ctatatctaa agtagtttta gaaagtataa
    57841 gcttaagtga tgaaaaatat aaggagattt tttatcttta tataatgtat tcttataagt
    57901 acataagata catatatagc aatttaagtg attttgaatt aataaaattt gtttgtaatg
    57961 atttagataa gttcactata gaatttaagc atctacttga tattaaaaat aaagtaaata
    58021 atgctaatga aaaattagag tatatacata gtatgaggaa attactaaat gagtatcctt
    58081 tttataataa aataataaaa atacttatag gtgaattaga agaaaatata aaagaaagtg
    58141 aagaatttaa ggatttaaaa gatagtttta ttattagtat agaaaatatg attgaaactg
    58201 gcaaaataaa tgatgcgaaa ttattggtag atgactattc aaaaacattt gtggatgata
    58261 taaagatttt aaatataaag ggaattttat acatgtttga aaataagctt gataaagctg
    58321 attttatgtt aaaaaaggca ttatcattag atgtagaaaa tgaagatact atatataata
    58381 tagaatactt aaaaagttta agataagtca aaaataggag aattgaagaa tgaatatacc
    58441 aatagtggtt aatgaaaaat ctaaagacat aataaaagat tggcaatata catgtacaaa
    58501 tgaagatata aaatttacaa gcataataat attgacatat aatcaaatag aatatactaa
    58561 attatgtatt gaaagtatta ggaaatttac cccaaagaat aaatatgaaa taataataat
    58621 tgataataac tcttctgatg gaacagttga gtggttaaag ggacaatctg atataaaatc
    58681 tgtgtataat aacaaaaact taggatttcc aaaaggatgc aatcaaggta ttgagttagc
    58741 tactggtgaa aacatattgt tgttaaataa tgatgtaata gttactccaa attggctcta
    58801 taatttgaat aaagctttat ggtctcaaga taatataggt gctgtaggac ctattacgaa
    58861 tgcatgttca tattatcagc aaattgatgt tgattataag tcaattgagg acatgtttat
    58921 atttgcacac aaaataaata aatctaatat taacctatgg aaatatagat tgaagttagt
    58981 tggattttgc atgttaataa aaaaaactgt tctggataat gtaggggttt tagatgaatt
    59041 attcacacct ggaaattttg aagatgatga tttatctttt aggatgatag agtcaggata
    59101 taagttatta ttatgccagg atacttttat acaccatttt ggaagtgtgt cttttaaaga
    59161 aaatcgtaat aaatatacag atataatgta tattaataga agaaaatttg aaactaagtg
    59221 gggatttaat ccagaatata gtggaatgat aagaacagat ttgatttcta aaatagatga
    59281 tagtttggag gcaaatatta atgttttaga aataggatgc ggtatagggg ctactctttt
    59341 agaaataaaa aatatatata aaaatgcatc aatatatgga atagaaatta gtgagtcagc
    59401 aggtaaactt gcaaagtata gtgcagatgt acttatagca aatatagaaa aagtaaaatt
    59461 ggattataaa aaagattttt ttgattatat aatattggga gatgtacttg aacatttgat
    59521 tgacccatgg aagatagtca atgaattgaa acaatatcta aagaaggatg gttatctggt
    59581 atctagtata ccaaatatta tgcatgtttc tgttttaaga aatcttgtgt taggtaattt
    59641 tacatatgaa gattcaggaa ttttggacaa gactcattta aggtttttta cattaaatga
    59701 gataacaaaa atgtttaatg atagtaatta cataattaat agtattacag ctactagtat
    59761 gccactttcg gaaattgata atagcttcat tgatacaata tgtaaggaaa gctgtaaaga
    59821 aatgagacaa cagtacgaat cttatcaata tatagtaaaa gcctctaaaa tcctagatac
    59881 taaaagatat tctagtatta gtatgattaa tttaaaatac gctttaatga gaattgataa
    59941 tgaattagat gtagatagta atttaagtta tatatttgat aattataata taactgaacc
    60001 atgttttgtt gatgatattg aatatctaat tgatacattt gtgataaata aagcaactct
    60061 attaaataga ttaggagttg aggcatttaa cagaggtcta ggagatttta gtataaacat
    60121 gtttatgaca gtattagaga tggatagaga tgatattgac tcaatatata atataacaag
    60181 tgtattatgt gaattagaag aatttgatac tgcttataat ttaattaaaa atagtagcga
    60241 aaaagtacag agtgaaagtg atatacaaga gatactttgt tttattgagg ggaagaaata
    60301 tgaataatga taagatatgt tttataacct gtgtaaatga tgaaaggctt tatgaagaat
    60361 gtcttgtata tataaatagc ctagacattc ctaaaaactt tagtatagaa acagtagctt
    60421 taagaggagc cgattctatt gcctctgctt ataataaagc tatgagagaa acagattcta
    60481 agtataaggt atacttacat caagatactt ttataattaa taaaaatttc atacatgata
    60541 ttttagagat atttaaaaaa aatacagatg tcggaatgat tggtgtagct ggagtaaaag
    60601 atatgcctgt gaatggaatt tggtgggatt ctgataaaaa agcagggaaa gtatatgata
    60661 gtcatacagg taaaatggat atttatgaat ttagtaactt tgaagaatgt tttaatgaag
    60721 ttatggctat agatggatta attatgatta ctcaatatga tgtattttgg agagaggata
    60781 agtttgatgg gtggcatttt tatgatttgt cacagagtat ggagtttaga caaaataaat
    60841 acaaagttat tgtaccaaaa caaaataagc cttggtgtat gcatgattct ggagtagtaa
    60901 atataaataa tggatatgat gaatatagga taaaatttgt aaaagagtac ataaatcttg
    60961 atttatatag agaaaatttt tatgaatttg ataagaagaa agcaagtacc atacttaaga
    61021 gtaataagca tgtaatggat tgtatgaaag aggagcttac agaacaatta agattaaaga
    61081 attacaagga tgttttaggt attgtttcta aaattgctca tttttctgtt tttaatcata
    61141 ctggtttgta ttcttgtgaa gaatgtgaga gagcattgct agaatgtgca aataatttac
    61201 cagaaataga aaatacgaaa ttatttacaa aaaaagattt aaaaagaaga aaagtattac
    61261 atgttttaag tgaaggatat tcaacaggag gtcatacaag actagttaaa aattggattg
    61321 aaattgacaa agactctgtt cattcagttg taactacatg gcaatcagat acactgccag
    61381 actggttatt aaaagcaata aatgatagtg gtggcgaagt tatatctttg gatttaattt
    61441 cagatgatta tttaaaaaga gctgtagctt tgaggaaaat agcttatgac tgggctgata
    61501 tagttatttt atatgttcat atgtgggata caatacctgt tgttgctttt ggagttgatg
    61561 ggaaaatacc tattttacat gttaatcact atgatagtac tttttggtta gggacaagta
    61621 ttgcagatat tgtcattgat tttagcgaaa ctagtcaatt aattacatta gataaaagag
    61681 gcatagataa atctgaaata ttaccaatgc cattaaaaga aaaagaagta ttttcaaaat
    61741 ctgaagttag aaaacgatat aatttagagg atgatgtgac aataatattg actattgcta
    61801 gtgaaagaaa atacaatagt gtaaatgata ttaattactt taaaattgct aaaacattga
    61861 ttgaaaatac agataataca attattattg ttgtaggagt tgatacagag aatctctatt
    61921 ggaaaacttt aagtaatgtt acagataata gagttatatg ttttggaaca acagcaaata
    61981 tagatgattt ttataagatt tcggatatat acctagagtc tttcatggta ggaagtcata
    62041 cttcaaaatt agatgcaatt ttatatgatt taattcctgt taagtttaaa aatgaagttt
    62101 caccaatttt tacaaatatg tggaatttta tagataagtt tgattataag aatataaaag
    62161 aggttataga catgataaga aggtacaata ctagtgaatt tgacagggat aagataaaag
    62221 caaaacaatc ggaaataaaa cattttgtta tagataaaca ttgtattgaa ataaaaaact
    62281 atatagacaa aatatataat gaagttaatc atcataaagt taagtatgat ttatataaaa
    62341 atgatgaaat cgaggactat cagctttttg tagcattata tggctatatg gtaaaaaata
    62401 aaaacatgtc ataggtggta aaatgaacaa tattcatatc aataaatttg aaaaaagctg
    62461 tgaaaaaaac tgggattatt ttgtagaaaa taaatctata aatggtacat ttctgcaaac
    62521 tagaaatttc cttaattatc atgaggaggg aaaattcact gaccactcat taataatatg
    62581 taaaggtgaa gaaatactag cagtcgtacc tgcatgtgaa acttatgaag atggtgaaag
    62641 aatatttttt tctcatttag ggagtacttt tggtggcatt ataataagaa gtggttttaa
    62701 tgatattagg catgtaaatg ccattacaaa taagttagaa atatatttac aggaacaaaa
    62761 tttcgataaa gtatatttaa aaaatacatc aagaatattt tgtaaagagg atacttcatt
    62821 actagaatat tttttattta agaataatta catgttttat gatgaattaa gtatgttttt
    62881 agaattaagt aagtataaag aagatatatt agccaatttt aaatacagta aaagaagaga
    62941 ttttaaatat tctttgaaga ataatcttac ctttaaaaga ctgtatagtg atgaagatat
    63001 agtttgtttt tttaatttac tttctaattg tttagatagt aaaggagcta aagtaataca
    63061 ttcagtagaa gaacttttgg agtttaaaaa taatagatta aaagatattg tagaatttta
    63121 tgcagtatat attgatgaca ttatgatttg tgggagcatg gtatttaact ttcaagaaag
    63181 agtttatcat actcaatatc tagcatcaaa ttcgacatat aataaatatt atccaatgat
    63241 atttttatac tataatctaa taaaagatgc tttagagaga aattttaagt atctttcttt
    63301 tggaattagc actctaaata gaggcaaata cttagatgag ggtttagcta catttaaaga
    63361 aggttttggt tcaagtgcaa tttgtaacag aacatactat aaaaatataa ataagtgaga
    63421 atcatgtttt atagagtttt tagagtatag taaatatcta gtactataat attacataaa
    63481 aataattagt ttataaagaa ggtttgtata tgaaaagatt aatgatttta ggctcaatta
    63541 agtattttga gaatattgtt atgtcagcta aaagagaggg aatatacaca attgtttgtg
    63601 ataatagaat tgatactcct gcgaaaaaga tttgtgatga agcaatagat gttgatgtat
    63661 ttgattttga taaaataaag aatatagcta tagataaaaa gatagatgga attttaactg
    63721 gatttacaga ttctttaatg aagccatatg tttatattgc aaatgaacta aatttgccat
    63781 gtgtgatttc ttgcaatcaa cttgaatctg taactaataa ggctgttatg aaaaattatt
    63841 ttaaaaataa tggaatacct acaactgatt attgtattat tgaaagtttg aaagatttag
    63901 aaaaaataca aggattaaat tttccacttg taataaaacc tgttgatggt tatggttcaa
    63961 gaggtgtata ttttgtatca aacatagaag aattaataga aaaatttgaa tattctaagg
    64021 aagcctcgca agatggacaa gtaataattg aggaatttta tgaaagtgag gaaatacaag
    64081 gactagcttg ggttcataat ggagagtcac atgtatttta tattggagat agagagcttg
    64141 taaatataca tacaggaaga gctggaaaac cagatagatt aatttatcca tcaaagtatt
    64201 gttttcaata tgaagaagaa ataaaatcca tatatcaaaa tataacgcag gcttttggca
    64261 taaaaaatgg acctctttat gtacaaatgt tagtgggagc atatggaata aaggtatctg
    64321 aagtaatgcc aagattacct ggaggttgtg actatatggc tatttctcaa ataacagatt
    64381 tagacatagg aagtttattt gcaaacttta gtgtgaataa tgaagtaaat tttgatgaaa
    64441 taaagaaata taatatgcat ctatcaaaat gtgtatatgc attaccaata tacataagac
    64501 ctggcacaat aaaaaagata aataatatag ataaaataga aaacttagat tatgtcactc
    64561 aatttctatt actagtaaaa gaaggagata caataggatt atatgaagat gagagacagg
    64621 attgtggaag agtttatgga gtagcaaata atgtatttga tgcaaatata aagaagaaat
    64681 gtattcatga aatgatagaa atacttgatg aaaataatga aaatatgata gaaaattttt
    64741 aaataaggga gtatgataga tatgaagcta ataactcatg aacaaattat gtcattaaac
    64801 atttctcaga aacaaggata cgagtggata aaagaatcct ttttaacaaa aaaagattgt
    64861 caattaccac caaaaataag catggtagaa gaagggcatg tattttacaa tgtaatgccg
    64921 tccctaaatc ataatcataa gattggtgga ataaaaatgg ttacaagatt gccagggaga
    64981 aatccaagtt taaattcaga gatatttcta tataatatgg aagatgctaa attaaaggct
    65041 attttagatg gcaattatat tacagctttt aggactgctg ctgttgctgc cttggctatc
    65101 gatacatttg ctaaaaaaga ttttaatact attgctatta tgggtttagg tgtaactgct
    65161 tctgctataa tgaatatgct ttcatatact cttaaagata aaaatataaa tataaagtta
    65221 cttaaatata aagaccaagc agagtcgttt gtaaaaaagt atgaaaaaaa tgaacaattt
    65281 agttttgaaa tatgtgacac acatgaagaa cttattgtag attcagatgt agttatttct
    65341 actgtgacat atacagatga atatttatca gaatttaaat ggtttaaaaa aggtgcttta
    65401 ttattacctg tacatgttat gggatttgga aatgttgatt atctatttga taaagtatat
    65461 ttggatgatt ataatcattt gaaacatcta gaaaatatag atagatataa atattcttgt
    65521 gaattttatg atgttttaag taaacaagct aaaggaagag aaaatgatga tgagagaata
    65581 atatcttaca atataggttt gtcaatacat gatatattat ttgcaaataa aatttacgaa
    65641 ttgtgtgaaa cactaagctg tgaagaaata gaattaaaag aaccaaaaga aaaatattgg
    65701 atatagttta tttcattcat atttacaata gtttattgta aatatgaata tgaagtaata
    65761 tattgttaga aaattagcat gaataggata gataaattta tggataaata taatttaatt
    65821 aggttcgtat ctattggaaa cgaatatata ggaaatttag ttgcattaga atcaaataaa
    65881 gcaatacctt ttgatataaa gagagtttat tacatatatg atgtaccaaa caatgtaaag
    65941 agaggatttc atgcacacaa aaacttggaa caagtactag tttgcctaaa tggaagtgtg
    66001 aagataaaat gttatgatgg tgtaaaagag actatattta atttacataa aaatgatgtt
    66061 ggattatata taggaacggg agtgtggcat gagatgtttg attttgatga aaaaactgtt
    66121 ttaatttcta tagcatctga acattataat gaagaagatt atattagaga ctataaagaa
    66181 tttttaaagt atttaaataa tgtgtagtta ttatacaatt agtgatgtat tttcttaatt
    66241 aaataaattt aagatagcca catagagaat agatagatta ggatgtgaat ttattgttgg
    66301 ttaaatttaa taatttatta aaacagttcc aaactcataa agaagaatat gaacagaaga
    66361 ttaaggatgt aatggaaagt ggatattata tccttggaaa tgaagtaaag cattttgaag
    66421 aagaatttgc taactatgta ggtagcaaat attgtgttgg tttagccagt ggtttagatg
    66481 cactgataat tgcttttaga gcattaggaa taagcaaagg tgatgaagta atcgtgcaat
    66541 caaatacata tattgctagt gttatgggaa tcagtataaa tggagcaaca ccagtatttg
    66601 tagaaccaaa tgaatattat aatatagatt catctaagat agaagaaaag ataaatagca
    66661 aaacaaaagc tatattagta gtgcatttat atggtcaagc aagtaatatg aaagatatac
    66721 aagaattatg tgataagtat aacttgtatt taattgaaga ttgtgctcaa tctcatggag
    66781 ctaaatatga tggaaaaatg acaggaacat ttggtgatat aggttgtttt agtttttatc
    66841 caagtaaaaa tttgggtgca tttggagatg caggagctat agtaactagt aatgaggaaa
    66901 tagctgataa agtgaaaatt cttagaaact atggctcaga aaagcgatat tataataaat
    66961 ttgtcggata taattcaaga ttggacgaaa ttcaggcagg tttattaaga gtaaaactta
    67021 aatatctaaa tgaaattgaa attgaaaaat caaaaatcgt agatagatat cttaatgaaa
    67081 ttaataatcc caaagtcata cttccaaaga tagaaaataa ttgtactcat gtatggcatt
    67141 tatttgtttt acaagttgaa aaacgagata catttatgga atacttaaag aaaagtggta
    67201 tagagtctat aatacattat ccgataccac cacatctatc tgaagcatat gcttatttgg
    67261 gatataaaaa ggggagtttt aatatagctg agaactattc agataaagtt gttagtatac
    67321 ctttatttat tggtatggaa gatgaagaag ttagttatat aattaaaatt ataaatgaat
    67381 attaaactac tcataataat atcaaaaata aattttttat atatcattta tttaaatttc
    67441 gttaattgac atttttaaat attgatatat tgtacaaata aaattgaaat atatggaaaa
    67501 atatagaatt aagatgagat ttgtcttata tttttataaa ttaaaaatat tttcaaagag
    67561 tttattataa aattgtaaac tatagtgttt ataatctttg taattattgg taattaaatt
    67621 taaaaaataa aatatttaat tatacacaaa aaagatattt aaaaaatttg ttatttattg
    67681 tatacttaag ttaaaactaa ataggcaaat ctagagaaat ctagtgacgc aaagctatag
    67741 ggactaaggt ttatatacat aaactatgtc agccagttgc caaaaagagt cctaggtgta
    67801 ttgtatacct aagaaaagtc tataatgact gcctttttgg cagtcatttt gttatggaaa
    67861 aatcacttta tttcagtaga aaatacacat aataacatat tttaccaatt ataacataaa
    67921 ataaagaaaa attatctcaa aaagttacta ttttacaaaa aagagcaact tttcgaagaa
    67981 atatttaaat acatattaaa atttttttat attttcatgg gacttcatta aaaaaatatt
    68041 agttttctta ctaaattgat acattattgt attaatgtaa tggaaatgcg attaatttaa
    68101 ataaataaag aagtcatttt ttgtaatata gcaacttcat tttttttatt aataaacaaa
    68161 atagataaaa tatattttga ggaggggtga gtgtgaatac atatgacatt ataaaaatgg
    68221 gattagatgc aactaatcta agaagtcaga caatagcaaa taatatagca aatataaata
    68281 ctgaaggata taaaagacat tatataactt ttgaagaaac actaaatgat gctatgccta
    68341 aaatagaaga aaaaacagac aattctaaag gtttaagagt agatggtaat aatgtagaca
    68401 tagaagaaga aaaagtaaat caagcaatgg caacattaca atataatgct ttgataagtt
    68461 ttgcaaatgg aaaaatagca atgaccaaat caataataag tgggaggtaa ttagaagatg
    68521 agtgtgttca gtggaatgag aatatctgct agtggattaa cagcagaaag tttgaaaatg
    68581 gatatcatat catccaatgt agctaatata aatgctacaa gaggtccaaa tggagaagca
    68641 tataggagaa aagttgtttc atttgaagaa aactatgata ataaattagg tatgctagga
    68701 gtaaaagtta catctgttga agatgataaa tcacctctta aagaagtata tgacccatct
    68761 catccagatg caaagccaaa tggatatgta gaatatccaa atgtaaatat attggtagaa
    68821 atgacagatt taataacagc ttcacgagca tatgattcta acgtagatac attgaatgct
    68881 cagaagtcta tgatatcaaa agctttggaa ataggaaagt agggataaat tatgccaatt
    68941 acacaaatta acaatttaat gcaaggtgca aagttagata atttgattgg agaagcaaag
    69001 ccattaactc ttaataataa agaagaaaaa aagaattttg aagatgtaat aatagatact
    69061 atagataaag ttaattttca acaagtaaca gcagatgata tgaaagaaaa atttataaaa
    69121 ggcgaagatg tttctatgca tgaagttatg ttaataggtc aagaagcaca actatctatg
    69181 caatatttaa tagaagttag aaataagatt catgatgcat atcaagaaat gaataaggtg
    69241 caaatttagc cttttgaaca ggagtaataa tcaatgataa ccagttttaa aaataagatt
    69301 aaggagcaag ttgctaaatc taggacaaag ataagttctt taaaaaaggg aactaaaata
    69361 gcattaggta taggggttat tgctatatgt ttagcgctag tatttacagc aatatctgcg
    69421 agcaaaaata agtatgcagt cttattttca gaactagatg ctcaagatgc tgcagatata
    69481 tcagcagagc tagagtctaa aaagatagat actaaggtaa aaggaagtac gatttatgta
    69541 cctagaacag aagttgataa gttaaggctg gagttagcgc caagtcttac aggtggttct
    69601 aaaggatttg cactaatgga tgatagttca tcaatagggc ttactgatga agaatttcag
    69661 attaagaaac aaagaatgat acaaggtgaa atagaaaaaa caataaagag ttttccacaa
    69721 gtggaagaca ctagagtaca tataacattt ggagagtcat cagtatttga gaaggaggct
    69781 gttccaggga gtgcagcagt ttatttgatg ctaaaaccag ggacgacttt agaaaaaaac
    69841 caaataaagt ctgttatggc acttatatca ggaagcgtta caaatatacc agagaaaaat
    69901 gttcaggtta ttgataacaa aatgaattta ttatctgaag gtattttgga ggatgaaaat
    69961 gttgatgaaa ataatcaacc aataaatagt ggcattagtg ttgataaaaa taaggcatct
    70021 gaacaagcat tgaataaaca actagaaaag tctattttag aaatgctaga accgatattt
    70081 ggtaagggaa aggtgaaagc tacagtaaat gctgatttaa gttttgatac agctgaaaaa
    70141 actgagataa aaattgaccc tgataaagtt gcaataaagg agacaaggtc gaaaaattca
    70201 tctaaatctg ctgcaaataa tgttcaaggt gtagatgcta acatgaataa ccaaggaaat
    70261 aacaatggta ctaatacaga agatagttta gatgagagtt ttgagtatga aacaggtaag
    70321 attgaaactc acactatagt tgcgcctgga gaacttaata gattgacagc ttctgttgca
    70381 atagatggaa aaatagctaa aggtgttcaa gctgatgtag aggatattgt taataatgct
    70441 ataggtatgg atggagcgag gggagatact cttagcgttg tttcaatggt atttgaccca
    70501 gaaggtaaga aagaggctaa acaagagctg gaagagatta aaaaagaagg atttaaaaag
    70561 aacataataa aagcagttat tggagtagta gcagtaatag cagttggagc tattggatac
    70621 ttaatatata agaaaagaag tgaacgagaa gatatggatt atgaagatga agattatatt
    70681 tctaaatctg attctgttga taggaaggat aatcctctat taggagatga tccagaactg
    70741 acattagagg aagctgttaa aatgtatgca gaagaaaagc cagaccaagt aacagagata
    70801 ataaagactt ggttaaatga gtaaggggga tagaaagtgt ccacggaaat tacaaatgtt
    70861 aatgcgtttg acttaagtga tataaatgaa agtgataaga atgagaaggt agaaggtgag
    70921 ggtttaaaaa aagctgctgt attattaatg acattaggcc caaatgtgtc tagtgatata
    70981 ataaaaagct tgcctgataa acaagttcaa aaaataggtt tggaaattgc aaatatttct
    71041 tcaatagctt caaaagaaag aagagaaatc ttaaaagagt ttgtagaaat aaatagggca
    71101 aaagattatg ttatagaagg tgggcttgaa tatgcaagag agttattaag taatgcttta
    71161 ggaaatgtta aagcaaacaa attacttgaa ggtatatcaa taaatacaag tacaaagcct
    71221 tttaaacata ttataaaact agatgtaaag aagatattag aaatacttag gaatgaaagt
    71281 gcacaaacta tagccgtttt acttgcacat atggaaccag aaaatggagc agaagtttta
    71341 tcatacttag attttgattt acaaaatgaa gtagctatga gaataagctc aatgggttca
    71401 atatctccag aagtaataaa ggtaattgaa agttcattag aagataaatt acattcaata
    71461 agtggtgatg attttaatga ttatggaggg gtgtctacct tagttcaaat attgattaat
    71521 tctgatagaa aaacagaaaa gaatattttg ggaactattg aagaacaaaa cccggattta
    71581 gcagctcaaa ttaaatctca tatatttgtc tttgaagata ttgtcaagtt agatgatata
    71641 agcatacaaa aagtgttaaa agaagtgact atgaaagatt tagtatatgc cttaaaaggt
    71701 gtagatgatg agatttcaga tgctatatat agaaatcaat cttctagagc agcagatgca
    71761 cttaaagaag aaatggaaat ggttggtgtt ataaaggtta cacaagttga ggcttctcaa
    71821 caaaagatag ctaatatcgt aagaaaactt gaaagagaag gtgccataac tattgtaagg
    71881 aattttggtg gtgactttgt tgaataataa gattattaaa agtaaaaaag cattatataa
    71941 aggagtgcta gatttaacca taatagataa agttagagtg ggagaatcaa atgtaaataa
    72001 agaaaatgat tgtagattta gggatgaggt aataaaagaa gctatagaat ctgctgagat
    72061 aaaaaagaat gagatattag aagaagcaaa aataaatgca caaaatatag aaaagcaagc
    72121 atatgaaaaa gggtatagtc aaggtcaaaa aaatggatat gaggatggat ataaagagtc
    72181 atatgatgaa tatacccaaa aagctaaaga tgaagcagat tttataaaaa atcaggcaaa
    72241 tttaatctta ttacaagcta atgagaagat gactgagtac ataaaagaaa aaaataaaga
    72301 gattgtaaga cttgctatta atatggccag ttctgtttta aaaaagcatt tcgaagatga
    72361 agaatctatg agtgatctgt taaagcaaat tattcaagat tatgaaggaa gtagttcatt
    72421 tatagtaaga tgtaattctt tctataaaga taatatagag aaagagtttg aattattcaa
    72481 agaggaaaca tcactaaaat caagtgtatt tgttattgca gatgatagta tagataaagg
    72541 caatgctatt atccaaaagg aaaatggaag aataatagtt ggtattgatt gtgctatgga
    72601 aaaactaaaa gaagaattgc ttggtgaata aagtatgtat aaaactgata tatctataga
    72661 ttttgacaaa ataaataaga aaatagaaaa tttagaactc actatatcag aaggtagagt
    72721 aaagaagata ataggactta ctgtagaagt tgaaggaata aaagcttttg ttggggagtt
    72781 atgcgttata tacaaccaag ttaataaacc agtgaattgt gaagtagtag gttttaaaga
    72841 taaagaagtc atattaatgg cgttgggaga attaacattg atagcacctg gttgtaaagt
    72901 tatttctaag gggatacctt taagtgttat gtgtagtgat aatttactag gtaaagtact
    72961 tgatggtctt ggaaatccta tagacaactc agatgctgtt ttaggagata gatacaacct
    73021 gaacaatgaa cctcctgatc caatgaagag aaaaaagata cgaaatataa tggaaacagg
    73081 tgtaagagca atagatgcat ttactacttg tggtgaagga cagagaatcg gcatatttgc
    73141 aggaagtgga gtaggtaaga gtacgactct aggtatgatt gctaggaatg caaaagcaga
    73201 tgtaaatgta attgctctta ttggagaaag aggaagagaa gtactagatt ttattgataa
    73261 agatttaggt gaagagggaa tgaaaaaatc tgttgttgtg tgtgcaacat cagataaagc
    73321 ccctctagtt aggttaaaag gtgctcttac tgctactgca atagcagaat attttagaga
    73381 ccaaggcaaa aaagtaatac ttatgatgga ttcagtaact agatttgcta tggcacaaag
    73441 agaagttggt gtagcaatag gagagccacc agctcaaaaa ggatatacac catctgtatt
    73501 tgcaatgttg ccaaaactta tggagagaac agggacatca gataaaggtt ctataactgc
    73561 attttataca gtattagttg atggagatga ctttaatgag ccaatagcag atacaacaag
    73621 agggatactt gatggtcata tagttttatc tagagacttg gcaaataaga atcactatcc
    73681 ttctatagat gtattaaaca gtttaagtag gcttatgaat gaaattgctt caaaagaaga
    73741 cataaaaata gcgtcttttg caagagacat gcttgctgag tatagagaag cagaagattt
    73801 gataaatata ggtgcatatg cttcaggtac aaataaaaaa atagatgaag caatctatta
    73861 tcatgagcat attattaatt ttttaaaaca agggataaat gaaaagagtt cttttaatga
    73921 aactataagt agtttgagaa gagtttttga ataaacaatt aaaaaaatat ttgaataggt
    73981 tgtgatggaa ttggataaaa actttaagtt tagattacag aaagttttag acttaaaagt
    74041 gaaagatgaa gaagagataa aaatggaatt tgctaagatt caacaaaaaa aaatagatat
    74101 agagactaat ttagaaaact tagaaagtaa ttatagcaaa tattctataa gtaaaaataa
    74161 cgatagtatt caaaatcaaa aaataactat taactattta ctagctttaa acaattctat
    74221 aatggattta tcagaagagt tggataagtc aacaaatgaa cttgaaaaag ctagaaagca
    74281 gcttataagc aaacagatag agagaaaatc attagaaaaa ttaaaagaaa aaaagtatgg
    74341 acagtattat aaagaagaaa atttaaaaga acaaaacact aatgatgagt ttgcaagtat
    74401 gagttattta aggaatagac aagttctata agaatagtag gaagtgagga tattattgta
    74461 tgaagataca agcagattct tataatatta ttaaagatat tggggataga aaaagttcat
    74521 ctaagaacaa aaatataaaa aataataaat ttgaagataa tctatataag gcaacagacc
    74581 ataaaaaagt taaagataga agggaagtaa gtaagcaaaa tattagctca gataaaccgg
    74641 aaaatagtga taatgagtgt catttagatg aagataaaag taatcactta gaagatgaaa
    74701 atacatatga aaaagattgt agtaagaata atgaagcttt gcagcaatta ctgaatcttt
    74761 taaaggataa ttataaaaaa gataaagata taaacactat attgaataat atagaaaaac
    74821 taagtatatc agatgaatta aaacaagaac ttaagcttaa taaaaatata ttaaataaga
    74881 ttgcaaatca aaattcagag ctaaataaag ctaatataaa taactctaat gatatagaaa
    74941 atttagacat agttaaatta agagaacttt tggaagataa tgctaataaa gatattgaag
    75001 taattactaa aactacagaa aatgatacca aaaatataga tttgtatagt tttgatagca
    75061 atagaatgga taatcttaac tcaaataaac agaaagatga tagctctgat attttggaaa
    75121 aactagcagg ggtaaatgga aataagagtg ggaattttgg acaagtaata aataaaaatg
    75181 ttgatatgaa taaaattaaa aatgataatg tcaatttaaa acagatagaa tcaaatgtaa
    75241 tggatgactc tataaaagct attaagcata tgaaaacaaa tgatatacaa gaattgacaa
    75301 ttaaattaag accaaaagaa ttaggagata tgaatataca attgttaaaa gatggtgaaa
    75361 gtatgagagc tgttgtgact gttttcaata aagatgtctt tgactctata aataaaaaca
    75421 tagcagattt aaagcaacat cttgaactta ctaatgtaaa tataaaagat gtatcagtgc
    75481 aaatgcacag cgataataca aatacatctg atacctttga taaagcattt gaacaacaga
    75541 atagacaaaa taaccaagaa aacatgagta agaagaatac aaatgataag cataataatg
    75601 tagtcataga agatgacatt aaagaaaata ctattgatga tgatagagta gatttactag
    75661 cttagtaagg gggaagtatt gcatgtacgg aatagcaacg gctttggcag gaactcaggg
    75721 aaactatagg tcaaataact taagccaact tgaaagaaat attaatgata ttgccaataa
    75781 acaagttaga agcaaaggaa atcagacagt aacagacaat gggactccaa ttataggaca
    75841 aggtgatgat gctgataaag acttgtttat gaagatactt gtggcacaga tgggtaatca
    75901 agacccaatg aatccgcaag acccaacaga atatgtgtca caattagcac agtttgcaac
    75961 tttagaaaag atgtcagcta tgaatgagaa gctagatgta cttttagcct taagtaattc
    76021 cactctaata aactctgctt taagtatggc aacatcatta attggtaaag aagtagaagt
    76081 atctggggaa gagaaagaag atgaaaacat tgtaggagta gtaaagtcca catatataaa
    76141 agatggggaa gtacacttag aattagaagt agatggaagt gacaaaactg taggtgttaa
    76201 atatgaaaaa ttacttaaaa taggagaggt agatgaaaat tcaggagaag aaaatgattc
    76261 ttcagaacac gaatctccag aagaagcata atattaaatg agaaaagagg taaattcatg
    76321 ataaaagcaa tgtattcagg tgtaagtgga atgaaagcaa atcaaacaaa attagatgta
    76381 ataggtaaca atgtagccaa cgctggaact acttctttta agaaaagttc agctagaata
    76441 acagattcat tttatcaaac tgtactatat gcaagtgctc caacagctgc attaggagga
    76501 acaaacttag gacaagtagg tgtaggtagt aagatatcaa gtataaataa agatatggta
    76561 caaggaaatg tacaaccaac taatagacca tctgatttaa tgatagatgg agatggatat
    76621 cttccagtag taagacaagg aacagttatg tatacgagag atggttcttt taatttagat
    76681 acaggattta aagcagctga tggtgctttg aagaatatta aaggtggaag acttgtaaat
    76741 ggtgatggat atatgttaca aggtatgatt tatgatgggg aatataaagc tgatactgga
    76801 gaatttgtta aaaagggtga tgctaaagga aaagagccta ttgtaatacc acttcaaagt
    76861 ataagtccta aggatggaac agttcaaaat gtactagagt ataatatatc aaaggatgga
    76921 acagtagaat ttttattgag tgatggtcaa agaactcaat ttgtctatga tatacctgat
    76981 ggtgctaagg taggagatac acctgctgga atagataaat taaaaggtac aatacaaaaa
    77041 gtgcaaacat atgcattcca aaatccagca ggactagatg cagaaggagg aaacttgttt
    77101 aaaccatcag caaactcagg agacccacaa atagcaggag ctacaggcac aataatacaa
    77161 ggggcaatag aagcttccaa tgtagatata gcagaagaat ttacagaaat gataatagca
    77221 agtaggtctt tccaagcaaa cagtaaaata atatcgacat cagacgagat acttcaagaa
    77281 ataataaatc ttaagagata gtaggtacat attatgataa gagtaacgga tttaaacgga
    77341 agaaaataca tattaaattc agaccttata gtgaggattg attctgtacc agaaagtagg
    77401 attctcctta caacaggaga aaaaaacctt gtaaaagagt cggtagatga gatagtagat
    77461 aaagtaataa agtataaaag aaaaataaat gtaggttata ttaggagcga aaaataatga
    77521 aaaaaacaga ttcactgaca tttattggac taatagtatc tacagtatta gttttagttg
    77581 gtgccgcaaa aggaagtagt tcagggttaa agaatttttt tgatgtttca tcaatattaa
    77641 tcacagtgct tggttcattt ggagctctaa tgattacatt tactatagat gatataaaac
    77701 ttataaaaaa tgcattacaa tactcattta agacaatgag tgtatcaaag ttagatttac
    77761 tagaacaatt taaaacactt tctaaaaaag ctagaaaaga aggtctttta tcaatagaga
    77821 gtgatgtaat gggaatagaa gatccattta tgaagaaagg gttagagctg tgtattgatg
    77881 gattggaaat caatgagata aggtccatct tagaactaga gatagattct gttgaagata
    77941 caaataacag atactctaaa ctatttaaaa cttggggaac ctttgcacct gcttttggga
    78001 tgttgggaac actaataggt ttaatacaaa tgttagctga ccttacatct ccagatttaa
    78061 tagcatctgg catgggaaag gcattaatta caacgtttta tggttcttta cttgccaata
    78121 tcgctttgaa cccaattgca tataatatag atgagaagac tgaaaaagaa atttatgtaa
    78181 aggaaatgat gttggaaggt ataataagca tacaatctgg ggagagcagt atagtagttg
    78241 aggaacgttt agccacatac ctttcaaaca atgagaagtt agaaattatg aaatccaata
    78301 aaaatacaga aagagcaatg agcaatggcg cgtaaaaaaa ataagaaaaa ggatgagata
    78361 aatccagatg catggctggc tacttatgca gatactataa cactgattct tacatttttc
    78421 gtattattgt atgcaacatc agttgtagat atggaaaaat ttaaaaacat agcagatgcg
    78481 ttaaaaggtc agttttctgg gacaagtata tttagtacgg gttcaagtac aagtgagaaa
    78541 tctccaatag acagtgtttt agtggataaa aatgataaat caaatttaga acaggattta
    78601 aaagataaag ttaataattc taacttacaa aatagtgtaa cagtcaaaga tgatagtaga
    78661 gggattttac tagagttaga tgattcaatt ttatttgact ctgggatttc tgagttaaaa
    78721 gtagatagta aagctactct taataaagta tatgacatga taaagatgat gaaaaataag
    78781 atagttatag aaggccatac tgataatgta cctgtaaaat caagtacaca tgaatccaat
    78841 tgggatttat cttctagtag agcagtaagt gttgtaagat actttgtaga agaaaagggc
    78901 atggacccta gactattttc ggcaacagga tgtggagagt atcaaccttt ggtttcaaat
    78961 gaaacagcaa agggaagggc tgagaataga agagttaata tacttataac tattgactag
    79021 gaggaagagg tttgttcaaa aataaaaaaa ttattataat tattttgata ctgattattt
    79081 tctcagcggg agcaggtttt tttatattag gagaaggtgg aaataaacaa gttgacaaga
    79141 ttgcagattt gtttattcca acaggaaaag gtaaagatga agtaaagacc gaaaatcttg
    79201 acttagaaga gatggtatta aagctagctg atgaaggtgt aagatattta aaagtatcaa
    79261 taactgtatc ttatgattct tcatataaag atatagaaaa aaatacgcct ttaattaggg
    79321 ataatattat aaattgtttt atggcaaaaa gggcagatga ttttactgct gataatttaa
    79381 agaacataaa agaagaaata aaaaatacat taaatataca actaggtgaa aatgtaattc
    79441 aaaatatata tttcacaaat atagtagtgc aataaaagga tggtaagaca tgatacttta
    79501 tttactaaaa atattaatat gtatcccaat aattttaata ttgatagtct tatctttaaa
    79561 aattagccaa ggaactttat taaaaaaata taatgataaa tatgtaaaaa tactagatat
    79621 tgtaaatata agtaaaaaca atagcataat aattttaaaa ataggtgaag aaggatgcat
    79681 tatggcatct tcttcttcta gcatgagtaa aataaaagat ttgaccaaag aagaaatatt
    79741 aaaaatagaa aacgacatta aagaaagaaa ttttaaaatt caagatttta aaatggaaag
    79801 aattaaaaag aaatatgatt tcagtagata tattggaaaa tttacaaaaa aggaagataa
    79861 gtaaatggaa ctattatcaa aattaccttt ggctgatgta ccatatacaa gttcaatgca
    79921 attgttttta tttctaactg cattaatgtt tttaccattt gtaatgttca tgatgacaag
    79981 ctttgtcaga atagttataa gtttatcatt tttgaaatca gcattaggag cacagcaagc
    80041 aattccaagt caaattttgg taggacttgc aatagtattg acaatattta taatgagacc
    80101 agtattaaat gagataaacg aaaaggcatt acaaccatat atgaaagaag aagttactat
    80161 ggaagaagct atgaaggaag cagaaggacc tattaaagag tttttattaa cacaaaccag
    80221 acagacagat ttagatttat ttgtagaaca ggctggttta aaagaaaaga aattaactcg
    80281 tgaaaatata ccactatctg ttgtagttcc agcatttgct ataagcgaac taaaaactgc
    80341 ttttcaaata ggttttttaa tatatatacc atttttgata atagaccttg ttgtggcaag
    80401 tgttttgatg tctatgggta tgtttatgtt gcctccagtc atgatatcat taccatttaa
    80461 gttattatta tttgtaatgg tggatggatg gaacttgata gtaaaaacat tgatattggg
    80521 atttggatag gagaaaaata gtatgagtga atttatgata atgagtgttc ttaaaaatgg
    80581 catgtttaca ggagctatga ttgcagctcc agtactcatt gtttctcttg tagttgggct
    80641 tttaataagt gtgtttcaag ctacaactca aatccaggag caaactttga cttttgtacc
    80701 aaaactaata gctattcttt tagtagtagc tattggtgga agctggatgt tacatactct
    80761 tattagtttt acttctgaga tattcaatat gatatctctt ataactaaat aagtaggata
    80821 catatgattt ttgtatttat tagaatagta gcattctttg gaacactaag aatactattt
    80881 ccagctggta cacctgcttt atttaagtca ctctttgcaa tcattatatc aatattgatt
    80941 tcaagtacta tgcaaattga atatgcaaca aatatagaca acattgtatt gtttacctta
    81001 tatggtgtaa atgagaccat aactgggatt atgcttggat atataaccaa cctatgtttt
    81061 tatagtatca gaatggcggg ttctatgatg gaccaacaga tgggattatc aatgattaat
    81121 atgtttgacc caaattcaat gactcagaca actttaattg ataatcttat gaactggaca
    81181 gcacttatga tattttttag catggatggt catcatgttt taattagagg tataaggtat
    81241 agttttgagt taattccaat aggaaaacca tttgtagata ataacataga ttatataata
    81301 aacatatttg tgcagtgctt tttaactgga ttcaaaatag caatacctat tgtactttgt
    81361 ctgcttatgg cagattttat tttagggctt atatcaagaa gtgtacctca attaaatgta
    81421 atgatagttg gtatgccatt aaaaatattg gttggtattg cattatttat catatcaata
    81481 cctttaattg tcaatcaaat aagtcattta ttaagtcaaa taccaaagat gtatgaggga
    81541 acttttgcac tagctcctat gttttttatg ggttcagcag ataaaacaga agaagctact
    81601 ccaaagaaaa agggagaaca aagaaagaaa ggtaatatag caaagagtag agaattaccc
    81661 gttgcaatga ctctacttgc attcacctta ttagtaccta cattattttc atatgtagta
    81721 gatacactta aatcttcgct taattatttt ttgagtctag atttttatat gaacataaat
    81781 tattcaaacc tagagaaatt agttatagca ggattaatgg atttttttaa gatattttta
    81841 ccaatagcta ttcccttttt agttttaggc ataatagcaa acctatttca ggtgggaata
    81901 ctgtttactg gtgaaacttt aaaaccaaat ttatcaaaat taaatccaat tagtggattc
    81961 aaaaacatgt tttctatgag aagtttgtca accttaataa aggatacagc gattatatct
    82021 attttggctt acataggata tacattcttc caagataatt atttagatat attaaagcta
    82081 ggtaacatat acctaccaac tttaatgtac acagttaaag acttagtata ttcaatattg
    82141 agtaagattt gtgtagctat gatagctata gcagttgcag attatgttta tcaaagatac
    82201 agtcataaaa agcaactgag aatgacaaaa caagaagtta aagatgaata taaaaactca
    82261 gaaggagacc cagaagtaaa agctaagata aaacaaaaac aaagacaaat ttcgtctcaa
    82321 aggacaatgc aagcagtacc aagtgctact gtaatagtaa ccaatccaac ccacttatct
    82381 atagcagtga gatatgaaaa agggaaagac caagctccag tagtggtggc aaaaggtgca
    82441 gattatctag cttttaaaat aagggaaatt gcaaagggaa ataacatccc tataatagaa
    82501 aacaaaccta ttgctaggtt gttatataag caagtagaaa tagaccaaga aattccagag
    82561 gacatgtatc aagcttttgc agagatatta gtggcagtat ataaaatcaa aaatagatac
    82621 aaagtcccca aaaggtagga taagtaatga ataagtttaa tttaaaggaa aatatggatg
    82681 taatagtggc atttggtgtt gtagggattg ttcttatgat gatagtgcca ttacctacat
    82741 ttatattaga tattttactg gcaattaaca taagtttatc tgtgctaatt ttactaatga
    82801 cattatttac aacaaatgta cttgatttat ctatatttcc aacagtgtta cttgtgacaa
    82861 cactatttag attgggatta aatctttcat caactagatt gattttaaca cagggatatg
    82921 caggtgaagt tatagaggca tttggaagtt ttgttgtagg tggaaattac gtagtaggaa
    82981 taattatatt cttaattata ttaattgttc aatttgttgt tataaccaat ggttctggaa
    83041 gagtatctga agtaacagct agatttacgt tagatgccat gccaggtaag cagatgagta
    83101 tagatgcaga tttaaatgca ggagttatag atgacaaaac tgctagaaaa agaagaaaag
    83161 agcttcaaca agaagcagat ttttatggtt ctatggatgg agcaaataag tttgtaaagg
    83221 gagatgccat agcaggtctt atagtaacag caataaacat aataggtgga attgtaatag
    83281 gagctgtaat gctgaatatg actcttatgg gtgcagcaca gacatatgta agacttacta
    83341 taggggatgg tcttgtaagt cagatacctg ctctattaat atctacatca gcaggtataa
    83401 tcgtaacaag agcttctact gatgaaaact ttggtgactt agtaggtaaa cagttaacgg
    83461 caataccaaa agcagtaatg atgactggtt gtgttctaat cgtacttggt atgatgcctg
    83521 gattacctaa accagcattt ttttcactag gtataggagc tatagcagca ggttactttt
    83581 taggaagaga aaagaaagat actttagaag acttggagtt tgaaaaacct gatatggtaa
    83641 acccagataa aaatgaagta gaagatgtaa caaatttaat aaatgtagaa tcaatagaag
    83701 tagaaattgg atatggttta atatccttag ctgatgaatc agcaggagga gatttacttc
    83761 agagaatagc atcaataaga agacaatgtg caatagatat gggtatagtc gtacaaccca
    83821 taagaataag agataactta cagttaaatc caaaccaata caatataaaa ataaaaggaa
    83881 atgtagttgg tgggtatgag ataatgccta atatggtttt atgtatggac cctatgaatc
    83941 aaggatttag tatatttgga ataaagacaa ttgaacctac ttttggaata gaggcacttt
    84001 ggatagaaag tgacaaggta gaagatgcag agttaaaggg ttatacagtt gtagattcat
    84061 ccacaatact tataactcac ttactagata taataaaatc aaatgcacac gaattactgg
    84121 gaagacaaga agttaagact attatagatg cagcaagaga aaattatagt gctgtagtag
    84181 atgaattgat accagatttg atgacacttg gtgaaattca aaaagtactt aaaaatctgt
    84241 taagagaaaa agtaaatata aaggatagag ttacaatact tgaaacgctt gcagaccagt
    84301 ctagaaatac aaaagatata gagcttctta ctgaatatgt aagaatagcc ttggctagaa
    84361 gtatttgtaa taatttagtt gatgatgata agacaattgt agtggctaca ttgtctccag
    84421 aaactgaaga gttggtatct aataatttac agaggtctgt aaatggaact tatccagcag
    84481 ttgacccaga aaatacaaat aaaatatttg aaagtataca agaagtaatg agcaatgtat
    84541 atttcaatca taatatacca gtaatagtag tatcaccaaa gataagagct ccatttagaa
    84601 aattagttga aattgtatat cctaatttga ctgtattatc attaaatgaa atacctaacg
    84661 atattaaaat caaagcagaa ggtgtggtga gcatatatga tgacgaaaaa gtatacagct
    84721 aacacaattc aggatgctat gaatctagcc aaaatggaat tgggtgacaa tattacatta
    84781 atagataaaa aagaggtaag aaagtctgga atacaaggta tcttttcaaa aaaagatatt
    84841 gaactaacta ttggatggga aaagagagaa aatgtagaac aaaaagattt aaaacgagag
    84901 atagaacaat taaagtcaat tattaacaat atggggtttg ataataagaa tgataatgat
    84961 atagataaaa tatgtaaaaa tctactaagt ttagaattga atgaagaaat tgtagaatct
    85021 ataagaattg atttgcaaga aatgaagttc aatggaatcg atacaagtaa aaatttagta
    85081 gaaattttaa agaaaaagat taaaatagaa aatcaagtaa taaatggtaa aattgctcta
    85141 gtcggaccac caggagtagg taaaacgact acaatagcaa agttagccgc aaaattagtt
    85201 tttgaagaaa ataaaaaagt aggagtaata actatagata catacaggat tggtgctgta
    85261 gaacagttga agatatatac tgatataatg aatattccct ttaaaggggt aataagtcct
    85321 gatgaaatgg aattggcact tgacgagatg aaagattgtg atgttgtatt aatagataca
    85381 acaggtagag gctacaaaaa ctctatgcaa atactagaaa tcaaaaatct tatagataaa
    85441 gctgaaactg ataatataca tttagttttg aattgtacaa caagagaaag tgatacaaaa
    85501 gcaattatag attcatatag aaatgtgaat tttaaaagct taataattac aaagctagat
    85561 gaaacaataa catatggctc aatatttaac ataatgaatt atgcgcaaaa accaatatca
    85621 tatattacaa caggacaaaa tgttcctgat gatattataa aacctaatga agataagatt
    85681 ataaggttac tattaggagt tgaaagtata tgatagacca agctcaaatt ttgagaaaaa
    85741 tagcaatgga aaaaagagga cttgatgagt ttatagtaga gaatgagaat gtacctaaga
    85801 taattacaat agcttctggt aaaggcggtg ttggaaagag caatctcgct acaaatttgt
    85861 ctatatgctt gaccaaactt aataaaaaag ttcttatatt agatgcagac ataggaatgt
    85921 ctaatataga tataataatg ggggtaaatg taaaagggac tataattgat gtaattaatg
    85981 gcgaaaaaaa tatagaagat ataatttcac agactaagta tggtgtaaat ataatatctg
    86041 gaggttctgc ccttaatcat atagaagact ttacagaagc tcaaagaaat aagtttatac
    86101 atagcataga acaaattcac gatgtggatt ttataataat tgatacagga gctggtatga
    86161 gcaaaagcct attgtcattt atatactgta gtactgaatt tttcttaata acaactccag
    86221 aaccgacatc attgactgac gcatatagtt tattaaaagc tataagtaat tttggcataa
    86281 agagaagtgc aaatataata atcaataggg taattgattt agaagaagca aaatctacat
    86341 acaaaagaat aaaaatggtt gtggacaaat ttctaaacct taatctagaa ttgtatggat
    86401 atataataga tgacaaaaaa gtaagtttat gtgtaaaaaa acagatacca ttcattttag
    86461 aatatcctac aagtattgca tcaaaatgta ttatagccat agcaaagaga atggtaagtc
    86521 agtcaagaga agatattgtt gataataaaa gttttctaag aaagatgttt agtatattta
    86581 aaagctaagg aggcgtagtt aatgaataga gaagaattaa taaaagagaa tatgcctctt
    86641 gtaaagagta tagcaagaaa attttttata ccagggaaag gttttgaata tcaagactta
    86701 gtaaatagtg gagtcattgg attgattgat gcaataaata aatttgatgc tgaaaaaggg
    86761 gcaaagtttt ctagttactc ttatataaaa ataaaatctg ctatattaga tgaaattaga
    86821 aatcaaagtc caatttctaa acataatcta actaaagtaa ataagtataa tagagtggta
    86881 gaaaagttgc aatctaaatt attaagagag ccaacttcat atgaaattgc aaaagaatta
    86941 aaggtttcag aaaaagaact tcatgatatc gaaagtaata tagatatgtt aaatattgta
    87001 tcattaaact atgtaatctt tgaagataca aatgaaactg tacaagatgt aataagtgat
    87061 agagaagagg aagctccaga aaatataata gaagaagaag aaaagttaga gattctatcc
    87121 aaagcaatta gtaatttaaa tgaaagagaa aaattaatac tttcactgta ctattatgaa
    87181 gatttaaatc taaaggagat agggaaagtg ctaggtgttt cagaatcaag agtttcacaa
    87241 ctccacagaa aatcaataag aaacttaaga aacaagataa aagaacttaa atattctata
    87301 tagatggtga ttgataagat gatacaaatt atattaatag tagtgtctat tttaataata
    87361 gtaactatag ttataaattt acataaggaa tcaaaaaata aagataacag atactttgat
    87421 aaaatatttg agcaagaata tgaagcaaat gatagattaa taattgcaga aaaaagaaga
    87481 ttctttttag acaaagtact tgcagaaaaa agaattaata gtcatataaa agtttatgaa
    87541 aattataacc taataaaggc taatgaagaa agaaaggaaa acatatttat agaatcaaaa
    87601 aaggaagtaa gacaagaaaa tgagttatct cttaaagtga aacatcttat aagttcaggt
    87661 tatgatgatg tggaaatatg taagatgcta gatattggaa agggggaact gttattaata
    87721 aggagttgtt acaaaatata aggatattat tatgtgataa aaaagtattc ttaggaatag
    87781 gtattggtat attaatatca actttatgta taatgctttt tagtaataat gatatgagta
    87841 aggcagagat agaaaaaaag gccagagcgt taggcatgaa atatacaagt gagatgaaag
    87901 ttttagaata gatgatattg tttagacatt tacaaaaaag gaggtcataa tgtgataaga
    87961 ggattatata cagcagtttc tgctatgata actaatcaac aaaagcaaaa tgttgttgtc
    88021 aataacctag ctaatatgga tacaaatgga tataaaagta aacaactttt gactaaaagt
    88081 tttgatgaat taaagttgga gggttacaat aactacgcaa atggaactaa acaaaagcag
    88141 attataggcg atataagtcc aggtatttct atggatgaaa ctatcacaaa ttttaatcaa
    88201 ggacctataa aaactactga taataaaatg gatgtagcta ttcaaggtaa gggatttttt
    88261 ctagttagtg atgcagctgg aaatcaattt tacactagaa atgggaactt cagaacagac
    88321 aatcaaggtg accttataac aagtgaaggg tattatgtac ttgggacgaa tactgctact
    88381 ggtgctgtag gtcatataaa tgtaggaaat caaaaatttg aagtggcaaa agataataca
    88441 atatcactag acggaaatgc tatgtataaa tttaatattg tagattttaa taactacaat
    88501 aacttaaaaa aagaaggaga taacttatat tctggaggag gagctatgaa agtaaataat
    88561 gcacttacaa tacaaagtgc gatagagtcc tctaatgtag atatggtatc tgaaattaat
    88621 aatatgatga caatatcaag agaatatgag gcaaatcaaa agataattca ggctatggat
    88681 tctaaattag caaaaatagc aagtgagatt ggttcagtaa gataggtaat aggaggtgaa
    88741 tttttatgta cacaatgttt tatactagta aaacagcttt atcagcaaat caaagtaaac
    88801 tagatataat atctaacaat atagcaaatt ctgattcaac aggatataaa aaaataaata
    88861 tggaatattc tgatttagtg gatgaagtac taaatagacc atcatatcct acaaatggca
    88921 atgatatatc gactggaaca ggagctaagg caacagctcc catgagaaat tatgaacaag
    88981 gtgcattggc tcctacagat agtaagagta atttggcaat agatggagaa ggattcttta
    89041 gagtgataag aaaagatggt acatatgcat atacaagaaa tggtggattt aatgtagatg
    89101 ctttaggtaa gattgtagat gataatggaa atatattaga tgtccaattt gatgcaggat
    89161 ataactataa taatacaaat atagatagta caaacctgac agtttcaaga aatggagagc
    89221 tattcgtaga taataaaaaa ataggaaaaa tcaacttata tcaacctata ggaactcaaa
    89281 attttatatc tgaaggagat agtttattcg tcgcaactga tgcagcacag attaaacaag
    89341 ttgagaaagt cgatatagtt caaggctata gagagaggtc aaatgtttct ttacaagaag
    89401 aatttgttga cttaatagcc actcaaagag catttcaaat gaactcaaag ggaataaaga
    89461 cagcagatga aatgtggcaa atagcgaata atttaagagc taaataatta tgtaaggtga
    89521 ggaatattca tgaatgatat gaaagagtac gattttggca aacctcagag ttattcaaga
    89581 gaaaatttaa cttgtttaaa ttttttatta gctgaatttt gtaaaaaata taaaaactat
    89641 ataatttatg aacttaagtg taattctagt atggttgttg atagtataga ccaagtaaat
    89701 tatcagactt ttttagatag agtaacttct tcatgcgtag tagtacaaaa tgcaatggaa
    89761 cctgttatga aaaacttttc ttttagaata gatagaagtg tagctgatat gtggattgat
    89821 ataacttctg gaggaactgg agttgtaaag gataatgata gagaacttac agaacttgat
    89881 aaaaaagtgc ttttacacct tatggatgat ttagtaagaa atatgcactt atttgagggt
    89941 tttgaaaata tacaagtatt ggatacacat acacatatta atttgccaca attatgttct
    90001 ccaactgcac cagtatgcgt tgtagattta aaagtgctaa atgataatga gtatattgga
    90061 agtgtttctc tttgctttcc atacaatggt ttagaggctc tgttagaaag tatatctgtt
    90121 atagaatttt ttgataatga tgtaggagat gattcagagg aatttaccca aaaaatctat
    90181 aatagtgtat ctaatataga attatcagtt attgcagaga taggaaaagt atcaattaat
    90241 gttgaggatt tactcaaact agaagttgga gatgtaatag taacaaataa aaaaataaat
    90301 gattatatag atatatttgt agaaaattca aaatcataca cagcaacacc aggatttata
    90361 tcaagtaaaa aaggtgtaaa aataaatgat gctgttggaa aagaggtgta gttatggaca
    90421 atagtaatct tatttcagat gaagaagcaa agatgttatt aaatgataaa gttgaaagtg
    90481 aagttgatgg agatgatgag ttagccttag atgtctctga agttaatcga aataatatac
    90541 aaaggatact tgaccttaag ttagaattat ctgtagttat aggaagaact aagatgactc
    90601 taagagatat tttaaattta cataaaggtt ctcttattga actggatact ttggcagacc
    90661 aagatgtgga aatactaata gataataagg ttttggctta tggaaaagta gtggtggtag
    90721 atttaaattt tggtgtaaag ataacaagta tagtaagtga agaagacatg gtaaaatctc
    90781 ttgtttagag ataacatcaa ttaatactta aggcatgagg tggtacttat tttaaactta
    90841 aatgatataa aaaaagatat actaaaactc aataaaaaag actcatgttc tctatataat
    90901 gaatctatta ataatataaa taataattgt atatcaactg gattaaaatt tttaaaacaa
    90961 gcaagagatt taaacccaga tgatgttgat atattaaatc tcacaggtct tgtaaactta
    91021 ctaaaatgta attttgatga tgctgtagaa agtttctata agagttcact ttgtgaaaaa
    91081 actgctctgt gtgaaaagta tataaacatg cttacatctg aggagttttt aattttcttt
    91141 gaaaagtaca atcaacttat aaggttaata aataaaggaa tgtataaaga agctatacaa
    91201 gggtttaaac ttataactga ccaatactgt gatttaatag aaccttatga gttgcttgcc
    91261 ttactgtatg acaaggaaga agaatatata aaatttgatg aatgtttaga agtattgaag
    91321 attattgata aggaaaatta tttattaaat caagaaaata acacattata tatgtataat
    91381 agtgctaata tacaaactaa aagtaatata aaagtagatg attataagaa tgagttctta
    91441 cataaaagta gtagtaacca taaaattaat gattataatg atgtaactaa agaaaagagt
    91501 tcaagcaaaa accagaaaag ttcagattct aataagaaaa aaagtattaa aaaaaataag
    91561 tacctatata tagccataat attaggaata ttattagtag gacaaagtat atatagttct
    91621 tataagatga ataatttaac tgataaaata tctaaaaata atgaaactaa aaatgaagag
    91681 tcaatagatt ctaacttgac taagaaagaa aataatatag aagaaaatga agatatgagt
    91741 aaggcttcta atataaataa gagtaataaa aacattaata aagaggatga tgtaaaatct
    91801 aaaaaaaata ctgtagaaaa tgaagatgat aaagattctg acttatttac agaggatgaa
    91861 ttgatgtcta aggcgaatac tttaaaatca gaaaagaaaa ctaaatcttc tatacaacta
    91921 tataagaaag ttgcagatat aggtgaaaat aaagggaata catctgaagc aacttatcaa
    91981 gttgctattc tcagtgaaaa gttaaaagac tataaaactg ctgaagaata ttataaaatg
    92041 tatgtagaaa attactcaga aaaagatgct tattttgatg aatcttatta taatttaggt
    92101 atgatgtact ataacaatgg tgatttaaaa aattctaaat tgacattaaa gaagttggtt
    92161 aataaggtac caaatagtat gtataataat tcaaaagtaa aagaaatttt aaaagaagaa
    92221 tagttaaatt agataagaaa aataaattat tttattttaa aataatatga ttataatata
    92281 ttgacatacc attttaaaaa atttaaaata ttaattataa aatgaggagg gataaaaaat
    92341 ggaatttgaa aaagggagta tttcaattca tacagaaaat atttttccta taattaaaaa
    92401 atggttatac tctgataaag acatatttat aagagagctt ataagtaatg gatgtgatgc
    92461 agtaagtaaa cataaaagac ttgtatcact aggagaaata tcagaaaata agtcatcaga
    92521 ttataaaatt acagtatctg taaataaagg agaaggtaca cttaaattta ttgacaatgg
    92581 aattggtatg actgaagaag agataaaaaa atacataaat caagttgctt tttcaggtgc
    92641 tgaagatttc tttaataaat ataaggataa aatggaagag tcaaatgata taataggtca
    92701 ttttggatta ggattttatt ctgcatttat ggtgtctaaa aaggtacaaa tagatacact
    92761 ttcatataca gaaggagcga ctcctgtaag atggatatct gaaggtggta cagaatacga
    92821 aatatctgaa tcagatgcta gaaatgatag aggaactact ataacactat ttattgatga
    92881 tgatagtaaa gaatttttag atgaatttac tgtaagagga ataataaata aatactgttc
    92941 tttcttacca gtagaaatat atttagaaga tgttgaaaga ttagaaagag aagctaaaga
    93001 agcagaagaa aaagctaaaa aacaaaagga agatggaaaa gaagagattg tagatgcaaa
    93061 agtaattgag cctttaaacg acactaatcc tctatggtta aaatctccaa aagactgtac
    93121 tgatgaggag tataaagagt tttatagaaa ggtattcaat gtatttgatg aacctttatt
    93181 ttggatacat ttaaatgtag attatccttt caatttaaag ggtatacttt acttccctaa
    93241 attaaaaaat gagtttgaac tgactgaagg aaaagtaaaa ttatacaaca atcaagtgtt
    93301 tgtggctgac aatataaaag aagttatacc agaattttta cttttattaa aaggagttat
    93361 agactgccca gacttaccat taaatgtatc aagaagtttc ttacaaaatg acagggatgt
    93421 aagcaaaatt tctaaacata taattaaaaa agtagctgat aagttgaaat ctctttgtaa
    93481 aaatgaaaga gaagaataca ataagttttg ggatgatata caaatattta ttaaatatgg
    93541 ttgcttaaaa gatgaaagtt tctatgagaa ggtaaaagaa tgtatattat tcaaaactat
    93601 tgatgatgag tatataacat tacaagatta tttagaaaaa tgtaaggaca aacatgaaaa
    93661 taaggtattt tatgtaagtg ataaggaaca gcaatctcaa tacattaaac tatttaaaga
    93721 atacgactta agtgctgttg ttttaaactc atcaatagat actcatttta tttcatttat
    93781 ggaatataaa gagaatggag ttaagtttaa tagaatagat gcagaccttt ctgatgtatt
    93841 aaaagataaa aatgaaaata aagatagtga agaaaacaaa gaagagatag ctaagataga
    93901 aggtctgttt aaagaagctg tcggagaaag agtgaagaat tattctgtag aaggattgaa
    93961 aaatgaagac actccagcta tggtattagt atcagaacaa tctattagaa tggcagaaat
    94021 gcagtcaaga tttgctggaa tggatttagg aatgaatttt gaggaagaaa aaactcttgt
    94081 aataaatgag aacagtccaa taatcaaaaa attggtttct cttaaagatg atgaggaaaa
    94141 gaaagataag attacgctta tttgtaacca gatagcagat ttagctttac tttctaataa
    94201 agaattaaag ccagatgagt tagattcatt tgtacaaaga agtaataagc ttatgagtct
    94261 tttgatagaa ttataagatt gaatttgaat acattgaagc aaatttataa aaagtattca
    94321 atatcagtga taagaaagaa gtgtctcaaa atgaactttt cagttcatga ggcgcttttt
    94381 ttatgtgaaa ttaaatttat tttaatcatt tcaacaatga aaaatatatt tatctctatt
    94441 ttgagacaga ccattcttta tttttttatc tttggttaat tttaaatatt taagtaggtt
    94501 ctaataaaaa actctattta gttaaatata gttaacttga tatataataa gataatacat
    94561 ataattaagt aaaagattgt tttttagttt atgtttaaat acataattac actttgttaa
    94621 aaacataaaa ttattactaa agtagaaata ggaggtttta gaatgaattt taattataat
    94681 ttgccagtaa atatcttatt tgggagaggc agaattaatg atgtgggaaa agaagtatct
    94741 aaatatggca aaaaagtatt gatagtaaca ggtaaaaata gtacaaagaa aactggatta
    94801 cttgataaaa caatagattt actaaaagac tctaaaatag aatatgaagt ttttgataga
    94861 gtagaacaaa atcctcttat aactactata tattcaggag tagaaattat aaaatcaact
    94921 ggatgtgatt gtgttttagg tcttggtggt ggaagcatta tggatgctgc caagagtata
    94981 gctttttctt ataaaaaccc aggagattta aatgattata tatttggaat tagacaaggt
    95041 gaagaagctt tacctataat cttagttcca acaacatcag gtacaggtag tgaaggaaat
    95101 tgttttgcag tacttacaaa ttcagaaact aaagataaaa aatctataaa gaagaatagt
    95161 atgtatgcaa aagcatcaat aattgaccca gaacttatgg ttacaatgcc aaaacatata
    95221 attgcatcag ttggatttga tgcacttgcc cataatatgg aagcatatct ttctaatgga
    95281 agaaaccctc ttgcagatgt acaagctatt tatgggattg aacttatatc agaaaaccta
    95341 ataaaggtat ataatgatgt taatgattta gaagcatggg aaaaaataac acttgcaagt
    95401 actattggag gaatgacgat tggtacagcg ggtacagcct tgccacatgg aatggagcat
    95461 cctgtaagtg gtcttagaga tgtaatgcat ggaaaaggac ttgcagcttt gactcctact
    95521 attatagaaa gttcatggga tagtgatatt gaaagatttg gtaatatttc aaaggtatta
    95581 ggaggaactt gtgcagaaga ttgttcagat gcaattagaa atttccttaa aaaaatagat
    95641 ttagatataa aactaagtga tttaggtatt gaaaaatcag atatagattg gttaactgat
    95701 aattgtatga aaatatcagt tgcaaatatt aagagacatc caaagtattt taataaggaa
    95761 caaataaagg aaatgtacca taagtcttta taatttgact ttacatattt agttttatat
    95821 aaaaattata aattaataat aaaaaattat aagtaattaa tagtaaaaaa attgtgtata
    95881 gaagttatat atataataaa tttgtcttaa gcttttattt tactttctaa taaataaagg
    95941 agtttaaaaa agggaagatt aattaagtaa tattttataa aattcttcga tttaaaaact
    96001 ttaagtagag gtgaaataaa tgtttaatcc caaaagaata atattcgaaa aaggaacttt
    96061 agacactgat gtgggtaaaa aaatatacaa taaatttaga gataataaag atgtagagat
    96121 aattaatata tcatcaaata ggattaaaga acatattcca ggtgaaaatc tatatgaaca
    96181 atatagaagt gggaagcaga ctatggtagt tgggattaga aaaagtctga agtttcaaac
    96241 atgtaaacct tctgctaatt atcaactacc acttgtcagt ggatgtatgg gaagatgtga
    96301 gtattgttat ttgaacacac aattaggtga taaaccattt ataagggtgt ttgtaaatgt
    96361 agatgaaata cttgaacaga caaaaaaata tattgaagat agaaagccac aggtaaccat
    96421 atttgaaggg gcagctacat cagacccagt tccagtagag ccatatacta attctttaaa
    96481 gaaaacaata gagttttttg gaagagagga aaaaggaaga tttagatttg ttactaaata
    96541 cagtgatgtg gatagcttat tggatgctga acataataat catacagaaa tacgatttag
    96601 tatcaatact cctagaatta taaatgaaca tgagcattat acagcctctg caaataaaag
    96661 aatagagtca gctgtaaaac ttgctagggc tggttatcag attggtttca taatagcacc
    96721 tgtgttttta tatgaaggtt ggaaagaaga gtataaaaat ctgattataa gtataaaaga
    96781 agctttacca aaggattttg ataaaaaaat tatatttgaa gttatatctc atagatatac
    96841 aactaaagca aaaaatagaa tactagagat ttatccagat acactattgc caatggaaaa
    96901 tgaggataga aagtttaaat acggtcaatt tggatatggt aagtacgtgt atgataaaga
    96961 acaattagat gatatgaaag agttctttct taaggaaata aatggagtat ttaaaaatag
    97021 tttaataaaa tatataatat agcaaatctt acctaaagat ttgctatatt ttatttttat
    97081 acattttatg tttataataa agcaatgaga tttagcacta tcatataaga aaattaaaaa
    97141 gggcactatc tatcaagaac attttttcta gttttgatac tataaaggat aagagtaaca
    97201 aaaatatcag gaggagttat agtatgcatg catgggagtc tattcaaatt acattggatt
    97261 taatagaagc aaatttatca gaagaaatat caattaatga attagcaaat aaagcaaatt
    97321 tatcaccatt ttactatcaa agactattta aaagattggt aaataagact gtaatggagt
    97381 atataaagct tcgtcgtctt gcaagagctt ctgaatactt agcagaacat aagaatcgta
    97441 tacttgatgt agctttaaat tttggatttg ctagtcatga gacatttaca cgttccttta
    97501 aaaaagcata tggattaact ccagaaaaat atagagcaaa tccagtaaaa ctcaatcact
    97561 ttattaagcc agaattaatt ttaaattatg ctatggttga tgaaggtgtt ccactgattg
    97621 cagatgatat agtgattgaa gtcaatcgta agacattgag taatccaagg acatttgttg
    97681 gaatagaaat agaagtacct atttgccagt tggtgggcgg tgaaacaact ggaattgcaa
    97741 tagtagagga attatggatg aaacttggaa gtcaaagaca taatatacca aatcaaattc
    97801 caggtggaaa tgaatttgct gctctttata tgggaaatgc aaaagaagga aattgtatgt
    97861 atatggcagg tgtagaggta gagtcaggta catcagtaga aggatatagt acttttgagt
    97921 tgccagctaa agaatattta gtatgtggat ttgaggcaga aaactttaat gagttagtta
    97981 attcagctgt ttttaaagca gataaattta tggaaagatg gatgaagaaa cataatttaa
    98041 caactactga ttttgcaatt gagatgtatt accccactac acctgaagca gcatatctgg
    98101 aacattggat tgttccggtt cctattgaac aatagaaagg atgatgaata tgaattggca
    98161 agatgcgttt tttcaagata ctaaacctac atttgagcaa attaatgaat ttattaataa
    98221 tccattatgg gacaagttaa ataaaacttt ggtttctact tataatgtag aacctaagct
    98281 tgaatacagt aaatgttcaa tgcaaagagg ttggaatgta aaatataaaa aaagaggtaa
    98341 atcactatgt actttatatc cacaagaagg ttattttaaa gctttggtta ttgtaagtga
    98401 aagcaataga gtagaggttg atttatttat taatacttgt tgtgattata tcaaaaaaat
    98461 atataatgag gtaaactttt ttaatggaag taaatggcta atgattcaag tagatagttt
    98521 attggtatta aacgatatgc ttgaactaat taaatttaga gcttaacata tagaacagga
    98581 tgaatatata agtcatcctg tttttatatt caatcagata aattaatata tagtctataa
    98641 tatgaataat ttagtaaatt taaatttact ttatctttct acattctttg caactaagtc
    98701 cacatgtgtt tttttcttgc atattagtag caccccaatt agcaaattgt tgtaaaatag
    98761 gcacaatact tttaccttta cttgttaatg tatattcaac ttttggagga atttcattat
    98821 attgtttacg atgaacaata ccagtagact ctaaatcttt taatgattga gctagcatag
    98881 tatttgtaat gccatccaat tttcttttta tgctactata tctaattgga ttttcaactg
    98941 ctaacataca tataataggt aatttccact taccaccgac aatgtctaat gcataacgga
    99001 ttggacaggt taattcttca acgtttttag ttatattttt ttcagtattt atactcattt
    99061 taattactcc ttagttatag tagtatcttt tttatacttt atcagaaaaa actgcatact
    99121 tgttttttct gtattttata ttaaaattat atcaaaggat aatccttaat acaagtattt
    99181 aaaaggggtg taagaattat gaaagcatta gaatttttga acaaggcacg agtttattat
    99241 ttagcaacag ttgatgagaa caatcaagca catgtgcgtc ctttaggatt tgttatggaa
    99301 tacaatggta aacttacatt ttgcacatct aatcaaaagg atatgtttaa gcaacttatt
    99361 tttaatccaa aggttgaact ttgttgtata gaccaaaacc ttaatactct acgtatttta
    99421 ggtgaagcag tatttgttac atcaaaagaa acacaagcta aggcacttga aataatgcca
    99481 agtttaggtc agatgtactc tgtaggagat ggtaagtttg aaatattctc tattgataag
    99541 gcaaaagtta gttgttgttc aatgagtggt aaaaagattg atgtagagct ttaattataa
    99601 ttgaatcgac atccatagtg aaaattaaga aaacgttatt actgaaatgt tgatagattt
    99661 aatttaaatg caataaaaag atttttaaat aatgaatatg taaaaatttt tagttattag
    99721 atgtgtattt caataatgat atactatttt gaataattac atactagaaa taagataatt
    99781 aaaatt
//
LOCUS       DAMJWU010000006        73100 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMJWU010000006 DAMJWU010000000
VERSION     DAMJWU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 73100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.006"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(121..894)
                     /locus_tag="PC460_000554"
     CDS             complement(121..894)
                     /locus_tag="PC460_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF2599460.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="PC460_000555"
     CDS             complement(894..1280)
                     /locus_tag="PC460_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2599461.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="PC460_000556"
     CDS             complement(1305..1622)
                     /locus_tag="PC460_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF2599462.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="PC460_000557"
     CDS             complement(1634..1894)
                     /locus_tag="PC460_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF2599463.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="PC460_000558"
     CDS             complement(1894..2190)
                     /locus_tag="PC460_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2599464.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC460_000559"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC460_000559"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF2599465.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC460_000560"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC460_000560"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF2599466.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKRYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC460_000561"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC460_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF2599467.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="PC460_000562"
     CDS             complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="PC460_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF2599468.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8082..9188)
                     /locus_tag="PC460_000563"
     CDS             complement(8082..9188)
                     /locus_tag="PC460_000563"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF2599469.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="PC460_000564"
     CDS             complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="PC460_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2599470.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11408..11545
                     /gene="rpmH"
                     /locus_tag="PC460_000565"
     CDS             11408..11545
                     /gene="rpmH"
                     /locus_tag="PC460_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2599471.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11623..11967
                     /gene="rnpA"
                     /locus_tag="PC460_000566"
     CDS             11623..11967
                     /gene="rnpA"
                     /locus_tag="PC460_000566"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF2599472.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11999..12250
                     /gene="yidD"
                     /locus_tag="PC460_000567"
     CDS             11999..12250
                     /gene="yidD"
                     /locus_tag="PC460_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF2599473.1"
                     /translation="MNKIIYILKEISKHLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12282..12989
                     /locus_tag="PC460_000568"
     CDS             12282..12989
                     /locus_tag="PC460_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2599474.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12958..13587
                     /locus_tag="PC460_000569"
     CDS             12958..13587
                     /locus_tag="PC460_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF2599475.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13699..15078
                     /gene="mnmE"
                     /locus_tag="PC460_000570"
     CDS             13699..15078
                     /gene="mnmE"
                     /locus_tag="PC460_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF2599476.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENVIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15176..17068
                     /gene="mnmG"
                     /locus_tag="PC460_000571"
     CDS             15176..17068
                     /gene="mnmG"
                     /locus_tag="PC460_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF2599477.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17061..17780
                     /gene="rsmG"
                     /locus_tag="PC460_000572"
     CDS             17061..17780
                     /gene="rsmG"
                     /locus_tag="PC460_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF2599478.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17943..18728
                     /gene="noc"
                     /locus_tag="PC460_000573"
     CDS             17943..18728
                     /gene="noc"
                     /locus_tag="PC460_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF2599479.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18863..19636
                     /locus_tag="PC460_000574"
     CDS             18863..19636
                     /locus_tag="PC460_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2599480.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19638..20501
                     /locus_tag="PC460_000575"
     CDS             19638..20501
                     /locus_tag="PC460_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2599481.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20534..21676
                     /locus_tag="PC460_000576"
     CDS             20534..21676
                     /locus_tag="PC460_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2599482.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="PC460_000577"
     CDS             complement(21810..22385)
                     /locus_tag="PC460_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF2599483.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /locus_tag="PC460_000578"
     CDS             22645..23937
                     /locus_tag="PC460_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2599484.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="PC460_000579"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="PC460_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF2599485.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="PC460_000580"
     CDS             24717..24980
                     /locus_tag="PC460_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF2599486.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="PC460_000581"
     CDS             24995..25180
                     /locus_tag="PC460_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF2599487.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="PC460_000582"
     CDS             25283..26479
                     /locus_tag="PC460_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2599488.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /locus_tag="PC460_000583"
     CDS             26693..26971
                     /locus_tag="PC460_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF2599489.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /gene="ssb"
                     /locus_tag="PC460_000584"
     CDS             27008..27442
                     /gene="ssb"
                     /locus_tag="PC460_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2599490.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /locus_tag="PC460_000585"
     CDS             27461..27688
                     /locus_tag="PC460_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF2599491.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="PC460_000586"
     CDS             27831..28151
                     /locus_tag="PC460_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2599492.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="PC460_000587"
     CDS             28159..29115
                     /locus_tag="PC460_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF2599493.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="PC460_000588"
     CDS             29126..31120
                     /locus_tag="PC460_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF2599494.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /locus_tag="PC460_000589"
     CDS             31121..31570
                     /locus_tag="PC460_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF2599495.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="PC460_000590"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="PC460_000590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF2599496.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="PC460_000591"
     CDS             33122..33430
                     /locus_tag="PC460_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599497.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="PC460_000592"
     CDS             33512..34801
                     /locus_tag="PC460_000592"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF2599498.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="PC460_000593"
     tRNA            35127..35202
                     /locus_tag="PC460_000593"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="PC460_000594"
     tRNA            35209..35283
                     /locus_tag="PC460_000594"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="PC460_000595"
     tRNA            35288..35363
                     /locus_tag="PC460_000595"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="PC460_000596"
     tRNA            35397..35471
                     /locus_tag="PC460_000596"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="PC460_000597"
     CDS             complement(35854..36846)
                     /locus_tag="PC460_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2599499.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="PC460_000598"
     CDS             complement(36855..37913)
                     /locus_tag="PC460_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2599500.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="PC460_000599"
     CDS             38038..39501
                     /locus_tag="PC460_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF2599501.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="PC460_000600"
     CDS             39603..40400
                     /locus_tag="PC460_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2599502.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="PC460_000601"
     CDS             40413..42515
                     /locus_tag="PC460_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF2599503.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="PC460_000602"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="PC460_000602"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF2599504.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43211..43522
                     /locus_tag="PC460_000603"
     CDS             43211..43522
                     /locus_tag="PC460_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2599505.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43570..43887
                     /locus_tag="PC460_000604"
     CDS             43570..43887
                     /locus_tag="PC460_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2599506.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44100..44801
                     /locus_tag="PC460_000605"
     CDS             44100..44801
                     /locus_tag="PC460_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2599507.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45025..46326
                     /locus_tag="PC460_000606"
     CDS             45025..46326
                     /locus_tag="PC460_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2599508.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46463..47557
                     /locus_tag="PC460_000607"
     CDS             46463..47557
                     /locus_tag="PC460_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2599509.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIMLLDFIEPWSKFKLILEGE"
     gene            47559..48692
                     /locus_tag="PC460_000608"
     CDS             47559..48692
                     /locus_tag="PC460_000608"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF2599510.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48710..49819
                     /locus_tag="PC460_000609"
     CDS             48710..49819
                     /locus_tag="PC460_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF2599511.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49884..50624
                     /locus_tag="PC460_000610"
     CDS             49884..50624
                     /locus_tag="PC460_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF2599512.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGYVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50629..51621
                     /locus_tag="PC460_000611"
     CDS             50629..51621
                     /locus_tag="PC460_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF2599513.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVLICGIAMQLTDSIKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51809..52690)
                     /locus_tag="PC460_000612"
     CDS             complement(51809..52690)
                     /locus_tag="PC460_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2599514.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52963..54351
                     /locus_tag="PC460_000613"
     CDS             52963..54351
                     /locus_tag="PC460_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599515.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54379..55332
                     /locus_tag="PC460_000614"
     CDS             54379..55332
                     /locus_tag="PC460_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2599516.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            55392..55616
                     /locus_tag="PC460_000615"
     CDS             55392..55616
                     /locus_tag="PC460_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599517.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55609..56121
                     /locus_tag="PC460_000616"
     CDS             55609..56121
                     /locus_tag="PC460_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2599518.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQEDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56153..57007
                     /locus_tag="PC460_000617"
     CDS             56153..57007
                     /locus_tag="PC460_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF2599519.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57541..58335
                     /locus_tag="PC460_000618"
     CDS             57541..58335
                     /locus_tag="PC460_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2599520.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58439..59245
                     /locus_tag="PC460_000619"
     CDS             58439..59245
                     /locus_tag="PC460_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2599521.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SITVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     AIHQLFNFLIGIINEDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            59307..59828
                     /locus_tag="PC460_000620"
     CDS             59307..59828
                     /locus_tag="PC460_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2599522.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            60243..62330
                     /locus_tag="PC460_000621"
     CDS             60243..62330
                     /locus_tag="PC460_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2599523.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            62345..62632
                     /locus_tag="PC460_000622"
     CDS             62345..62632
                     /locus_tag="PC460_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2599524.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            62659..63930
                     /locus_tag="PC460_000623"
     CDS             62659..63930
                     /locus_tag="PC460_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF2599525.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            63988..64587
                     /locus_tag="PC460_000624"
     CDS             63988..64587
                     /locus_tag="PC460_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2599526.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            64592..65497
                     /gene="dapA"
                     /locus_tag="PC460_000625"
     CDS             64592..65497
                     /gene="dapA"
                     /locus_tag="PC460_000625"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2599527.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPSKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            65603..66235
                     /locus_tag="PC460_000626"
     CDS             65603..66235
                     /locus_tag="PC460_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF2599528.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            66713..69220
                     /locus_tag="PC460_000627"
     CDS             66713..69220
                     /locus_tag="PC460_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2599529.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            69562..71724
                     /locus_tag="PC460_000628"
     CDS             69562..71724
                     /locus_tag="PC460_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF2599530.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNETTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRALNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            71729..72346
                     /locus_tag="PC460_000629"
     CDS             71729..72346
                     /locus_tag="PC460_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599531.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNKKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            72535..72879
                     /locus_tag="PC460_000630"
     CDS             72535..72879
                     /locus_tag="PC460_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF2599532.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT        27946 a         8681 c        12172 g        24301 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ctttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa atacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttaacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat ctctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 acaatacttt tattttagtt ccttctattt caactatatc tcctgatttt atcttttttc
     7801 ctcttcttaa ttctatttca tcatttactg tcactaaacc ttcttgaatt aaaaattttg
     7861 catgacctcc tgttgatgca atctctgcta atttaagaaa ttgatccaac tttatatatt
     7921 ctgattctat agttatttca gtcataatag ttcctccttg tcactatttt aaacaaattc
     7981 ctattgatac actaaaagta tattatacca taaatatgct tataaaagga gttgtctaag
     8041 attagaaaaa ttttctacaa aagacaactc cttcatgtct gctatatatt tgatgatatt
     8101 cttactggaa gcagtaagta aatataatta acatcatctg ttggtttaat tatacaagga
     8161 tttacatttg tagtaaattc tataaatatt tcctcattat ctatattttt caaaccttcg
     8221 ataaaatatc tagaattgaa agctatatct aggtaatctc catctaaatc tatttcaact
     8281 tcttcataca catttccttt ttctgtatta gaagtaattg ccattacttt atctcttata
     8341 gataacttta taagattatt tttttctgat tgagacaata aagacgctct ctctatacta
     8401 tttaaaagtt ccttagtatt taatttaact ctgctgttat gttctcttgg caataatttt
     8461 ttataatcaa taaaatctcc ttcaagcaat cttgtaataa ttttcgtatc attaattata
     8521 aaaatagcat ttttttcgtt aaatccaact ttaacattat cctctccaga aagcaaacta
     8581 tttacatcta taagtgtctt tccaggaatt atgactttta tattttcagt taaactatca
     8641 accgaacaac tttttacagc taatctatat ccatcaattg ctactaaatt taagtttcta
     8701 tctacaattt ctaaaagttc tcccattaaa acaggttttg tttggtcctg tgagatagca
     8761 aatacagttt gtttaatcat attttttagg atttcttgag gtatgctata taaatcttct
     8821 tcatttaatt ctggtaattt aggaaattct tttgctgcat aacctttaat tttaaatctt
     8881 gaatttacac aatttatgta aatattattt tctgaatcag tttctatctc aacaaaggaa
     8941 tctggtaatt ttctaattat atctccaaat agccttgcat ctacaacaac atcacctttt
     9001 tctatgattt ctgcttgtac ataagtttct attcctattt cagcatcata tccagtaagt
     9061 tttaattgac cttcttctgt agatataagt atacctttta ataattctat tgttgttttt
     9121 ccattaatag ctttttgagc aattccaatt ctatttgcta ggattttttg attacaaatt
     9181 attttcaatg tggataacct cctctggctt tttatatata aaaataagtt aaaatcatag
     9241 tagtaatagt agtaggtgtt gttgataatg ttaataagta tgtataacat tataaataca
     9301 aagatattaa catgtgtata agatgttaat atctttgtta ttttagactt gataatttgt
     9361 taataatttt tgttcaaaaa aagtattaac aggttattca agagatataa acatacaatt
     9421 gtgtataaat tatcccttca aatctgatat aattttgtct attttagtct taatttcttc
     9481 actttcttct atatccttag atactttgtc atgagcatga attactgttg tatggtctct
     9541 acccccaaac tcttcaccta ttttaggaag ggataaatca gtaagctcgc gcgttaaata
     9601 catagctatc tgtcttggat aagctattga acgtgttctt tttttagaat taaaatcttc
     9661 catctttaaa ttgaatacag aagatacttt ttcttttatt ctgagaacat ttatttcttc
     9721 atttttagaa gttgttataa tatcttttaa agcttctgta gcaagatcaa aagatattac
     9781 cctattgcta agagaagaat aagcaaccac tctagttaat gcaccttcta gttcccttat
     9841 gtttgactta atattttttg ctatatatga cataacctca tttggcacat ctatacgttc
     9901 tagttgagcc ttttttctaa gtatagcaat tcttgtctca aagtcaggtg cttgaatatc
     9961 agttataagt cccatttcaa atctcgatct tagcctatct tctagtgtag gtatgtcttt
    10021 tggcggtcta tcactagata taattatttg tttgtttgct tcatgaagag tattgaatgt
    10081 atggaaaaac tcttcttgag ttctttcttt tcctgctata aattgaatat cgtcgattag
    10141 taatacatct acgtttctat atttattcct aaattcttcg tttttatcgt cttttataga
    10201 gtttataagt tcattagtaa atttttctga agaaacgtaa actacttttg agtctttttt
    10261 ttgactaaca atatgatgac ctatagcatg cattaggtga gtcttaccta atccaactcc
    10321 tccatacaaa aaaagtgggt tataagcttt agctggagat tctgcaactg caacgcatgc
    10381 agcatgtgca aatctattac tattaccaat aacgaatgta tcaaatgtgt attttggatt
    10441 tagattgggg taaagaactc tataatttaa atctgtgcta tcagaattta aatcagcaac
    10501 ctctttttcg ctcaatacaa atttaatatt atattttttt aatgacagtt gactaatagc
    10561 atcttcaatt aaatgtaaat atctattctc taagatatct ttattaaaat cactaggagc
    10621 cagtaagatt aaatcattaa gatgtatttt taatggtacg atgtttttaa aaaaggtatt
    10681 aaaacttact gaagttaagt caccttttat taattgtagg gttttgtccc ataaagaaac
    10741 tatatccata atataaacct ccgaacagaa taattaatat tttccacaaa gttattaaca
    10801 gaaaagcaaa aatatatata aattttacag aataactaaa tttatggata atggtgttga
    10861 taaattaata atagtataaa ttattaaaaa aatatgaaaa gatataaaca atatgtagat
    10921 aaagttataa ataacataaa catacaataa atggtgatta taactagagt attgtcagta
    10981 atcttaaaag ttatacacaa aagtatccac aagctgtgaa taatttctaa taagttgata
    11041 acatttatgc acattatcaa caagtatatt tataatatca aaatatatgt caaacttcaa
    11101 tgatttgtaa ctaatttatc cacaatatta actaattgtt gataaaaata taaacatgtt
    11161 aaaattactt tatttgtgta tataattgaa aaaaataatt tagaataaaa ttaaatagat
    11221 ttttactagt attttcaata aaaaaaatac ataatatata tatggtagat tttgtgatat
    11281 aagcgtccca aaaactcgtt gacaacagta gtaaaagtat ttataattat atgtgttatg
    11341 tttactttga gggtaaagtt attattttta taaacggaaa ataaaatctg aaaggcggtg
    11401 caaaataatg agtaaaagaa cttatcaacc aaaaaagaga caaagaagta aagaacatgg
    11461 tttcagaaaa agaatgaaga cgtctaacgg aagaaacgtg ttaaagagaa gaagagctaa
    11521 aggaagaaat agattaactc attaattaca aaagggccgc tgcaaaggtg gcctttttgt
    11581 gtaaataatt acaattcata gctgtgttta tggaggttaa ctatggactt taataggact
    11641 aaagggttga aaaaagactc tgattttaga aaagtatata aacacggcaa atcttttgca
    11701 aacaaatatt tagtaatata tatacttaaa aataaatcag attatagtag agtaggtatt
    11761 tctgtttcaa aaaaagtagg aaaagctatt actaggaata gagtaagaag attaataaaa
    11821 gaagcctaca gattaaatat tgatgaaaaa attaagcctg gatatgatat agtatttata
    11881 gcaagggtat ctagtaagga tgctactttt aaagacatag acaagtctat aaaaaattta
    11941 gttaaaagaa cggatatttc tatttaattg tatgctataa atatggaggt taatgacttt
    12001 gaataaaata atttatatat taaaggaaat aagtaagcat ttatcgaatt tatgtattta
    12061 tttagtgaga ttttatcaaa agtatatatc tcccttaaag ggacctacct gtagatttta
    12121 tccaacttgc tcacaatatt caatagaggc gtttaagaaa tatggatttg taaaaggtat
    12181 gtatttaacg ataagaagac ttttaaaatg tcatcctttt catccaggag gttacgaccc
    12241 tctaaaataa aagagagtaa ctattaggag gtataaaaaa attgaatata ataggaaatt
    12301 tcttaggatt gattcttaag gtaatatttg aatttgttaa tcattatggt atttctataa
    12361 tactttttac gatacttgta aagattattt tattaccatt aacaattaag cagactaagt
    12421 caacaaaggc aatgcaagat atacaacctc gtataaaaga gatacaagaa aaatataaaa
    12481 ataagccaga aaaacaaaat gaagaaattg ttaagttata tggagaagct aaaattaatc
    12541 cactatctgg ttgtttacct ttattaatac agtttcctat attaatagga ttatttagcg
    12601 ttttgagaga accagttgct catggcgttt ttgcaaacaa agctgcattt ttagcagctg
    12661 acaatgggtt tttatggata aaaagcttaa catcaccaga ttatgtatta gcagtatttt
    12721 caggagcaag tgcatatgtc atgcaaaaag taatgacacc aaaagatcag ttacaaggct
    12781 ctatgaaagt aatgacatat gtaatggcag gtatgtcttt ctattgggga tttatattcc
    12841 cagcaggact tacattgtac tggacagtaa gtaacttatt ctcaatagct caatattact
    12901 taataatgaa tcctttaaaa gctaaactgg ctgctaactc taaggaggaa aaagttaatg
    12961 agaaaaagcc taaaattaat aaaaagtaag agtaaagagg aagctataag taaagctata
    13021 gcagagttga atttaaaagc agaggatata gaagtagaaa tacttgaaaa tccaagtaaa
    13081 gggtttttag gttttatagg tgctaaagat ggtacttatg aaatttttgt tatcgagagg
    13141 gaagtagatg tagctaaaaa ttttatagag gtaatgttga aaaatgcaaa tgttgatgcc
    13201 aaagtcaatg tatctcaaaa agataattta atcatggttg atattgaagg caaagaagct
    13261 gcaagtttaa ttggtagaag aggggaaact ttagattcta ttcaattttt gactggatta
    13321 gctcttaata aaataaataa agattctcat acaagagtac ttgtagatat agaaaattat
    13381 agatcaaaac gagaagaatc tttaataaga tatgctaata aagttgctag agaagtagcg
    13441 aaaacaagaa aaactaaaaa attagattat atgaatccat atgagagaag aataatacat
    13501 tcagctcttc aaaatgacaa atatgtaatt acatatagtg aaggaaccga tccatataga
    13561 agattagtaa ttgagtgtaa aagataaagt caacctccta aacattaggg ggttttattt
    13621 tttaatatta ttaaatcatt aatggttggt taaatgtaaa tctatatgta aaaaattatg
    13681 aattaagagg tgataaattt gtttatagat gatacaattg cagcaatagc tacagcacct
    13741 ggagaaggtg gtataggtat acttagaata agtggagaaa aagctttgaa ggttgcagaa
    13801 gaaatcttta aatctatgtc tggaaagagt attgaagaat acaataagag aacattaata
    13861 tatggaaata tagtggataa tgaaaatgta attgatgaag ttttattggc atatatgaaa
    13921 ggaccaaatt cctatacagg agaagatgtt atagaaatta attgccatgg tggatttatt
    13981 tcggtaaaga agatattaga attaatacta tcaaaagatg ttagattagc agaggctggt
    14041 gagtttacta aaagagcatt cttaaatgga agaatagatt tatctcaagc agaggctgta
    14101 attgatgtta taaaagccaa aactgatata gctcatgaag ttgctcaaaa tcaattagag
    14161 ggttctttat caaagaagat aagagaattg agagataaag tcactgagat attagctcat
    14221 gtagaagttg ctatagatta tcctgaggaa gatattgagc atataacata tcaaactctt
    14281 aaagaaaaaa ctgatgaact taaaaaagac attaaaaaat tatatgatac agctgaaagt
    14341 ggaaaaatac ttagagaagg cttaaagacg gtaattgttg ggaaaccaaa tgtaggtaaa
    14401 tcttcactac ttaattctat tttaggagag aatagagcaa tagttactga tataccagga
    14461 actactagag atgttataga agagtttgta aacataaaag gtataccttt aaaaattgtt
    14521 gacacagcag gtataagaga tactgatgat attgttgaaa aaataggtgt tgaaaagtct
    14581 aaagaatcat ttactagtgc agatttgata gtaatggttt tagatgcatc tagaaagttg
    14641 tcagaagaag atatagagat attagaaaaa cttaaggata aacaaacaat agtgctgtta
    14701 aataagaatg atttgaaaca agagattgaa gaagaaaaaa tacttaaata tgtagaaaat
    14761 aatagtataa taaaaatatc agctttacaa caagaaggaa tagaagaatt gcaagataaa
    14821 atagagtcta tggtatataa aggtagtatc aaaaataatt ctagtttagt agtaacaaat
    14881 tcaagacata aagatgcact tagtaaagca tataaatctg caacagatgc tttaattgct
    14941 ttggaacaaa gtatgccatt tgactttgta gaagtcgatt taaaaaatat atgggactat
    15001 cttggatata ttaatggtga tacagtaaca gaggatttac tagataatat attccataat
    15061 ttctgtattg gtaagtaatg tacaaaagta aacttgtttt tattatagag atttttaaaa
    15121 aatttgaagt gtttctcatg aaacattaat tttgaaaaga aaggaattat aaatgataaa
    15181 gtttgaagca ggaaaatatg atgttatagt agtcggagca ggacatgctg gatgtgaagc
    15241 tgctttggct actgcaagaa tgggatataa aactttgata ataacaatgt ctttggattc
    15301 catagcatta atgccttgta atccatcaat tggagggact ggtaaaggtc aattagttaa
    15361 agaaatagat gccttaggcg gtcagatggg attaaatata gacaagacat atatacaaag
    15421 tagaatgtta aatactgcca aaggtccagc ggtacactct ttaagagcac aagcagataa
    15481 atttaaatat catgaagaaa tgaaaaagac attagaagat gaaccaaatc ttgatatagc
    15541 tatggatgaa gttgtagaaa tacttcatga aggaaatgtt gtaataggag ttggaactaa
    15601 actaggatgt tcatttaaat ctaaagctgt aatattggcc actggagttt atctgaactc
    15661 aaagatatat atgggtgaag ttgcatttta tgaaggtcct aatgcattag ggtatgcaaa
    15721 atatcttaca gatagcttag ttgagcttgg ccttagaatg agaagattta agacgggaac
    15781 tccagcaaga gttcatagag atagtataga cttctctgta atgtctttac aagagggtga
    15841 tgaaaaagta actccattct catttatgaa tgaaaatata gaaaaaaaac aagaaccttg
    15901 ttatttaaca agaactacag aagaaactca aaaagttata ctggataatt taaaaaggtc
    15961 tgctatgtat agtggagtaa tagaaagtac tggtccaaga tactgtcctt ctatagaaga
    16021 taaagtagta agatttagtg ataaaacatc acatcaatta tttatagaac cagaagggtt
    16081 aaatactaaa gaaatgtata tacaaggaat ttcaactagt ttaccttttg aagttcaatt
    16141 agatatgtac aaaactataa aaggacttga aaattgtaag ataatgagac ctgcatatgc
    16201 tattgaatat gattgtgttg acccaactca actaaaaata agtttggaaa taaagggtgt
    16261 tgagaactta tttagtgcag ggcaatttaa tggaacttct ggatacgagg aagcagcagc
    16321 tcaagggctt atggctggta taaatgcagt tagaaagata gaaggaaaag agccatttgt
    16381 attagaccgt tcagaagctt atataggtgt cttattagat gaccttgtta ctaaaggaac
    16441 taacgagcct tacagaatga tgacatcaag agcagaatat cgactatatt taagacaaga
    16501 taatgcagat atgagattaa ctcaaaaagg ttatgatata ggattagtta aaaaagatag
    16561 atatgaaagg ttcctaaata aaaaggctgc tgtagagaaa gaatttgaaa gactaaaaaa
    16621 tgaaagagtt acaccaaaag aagtaaatag tttgttggaa gaaaaaggag caacaccaat
    16681 aaaggttgga atatctctat atgaattttt aaagagacca gaggttactt acgaattgct
    16741 tgaagaatta gggaaaggcg ctggagaaga tgtttcaaga gaagttaaag aacagtgtgt
    16801 aataataact aaatatgaag gttatataga gaaacaattg aaacaaatag accaatttaa
    16861 aaaacttgag aataaaaagt tggatgagaa aataaattat tcatctatag aaggtcttag
    16921 attagaagct agacagaaat tagatgatat aaaacctatt tcaatagggc aagcatctcg
    16981 tatatctggt gtttctccag ctgatatatc agttttactt atatacctag aacaaatcag
    17041 aagaactaga ggtggcaaag gtgaataata tagaacttct gaaaaacggg attgaaggtt
    17101 ttaatataga tacgaatgac agtatgttag aaaaatttaa aatctataga gagatattag
    17161 ttgaatggaa taaaaagatg aatcttactg gaatagaaga tgaaaaagaa gtgtatataa
    17221 aacatttttt agactctatt tcagctgtaa aaaatggata tataaaaaat gggatgtcta
    17281 taatagatgt tggtacaggt gctggttttc ctggaatacc attgaaaata tgtttagata
    17341 gcttagaact tactttgtta gattcgttaa ataaaagaat aaacttttta gaggaagtta
    17401 gtagagtact agagttagat aatattacat ttatacatgg aagagctgaa gattttggta
    17461 aaaatgaaga ctatagggaa aaatatgatg ttgctacggc aagagctgtt gctggattac
    17521 cgattcttat ggaattttgt gtaccatttg taaaagttgg agggtatttt atttgcttaa
    17581 aaggtccgaa tgctaattta gagctagaag aatcacaaaa ggctattgat gttttaggat
    17641 tggagtatat tgaaaaaata gatgtagagt taccagaggt agatttaaat cataatatat
    17701 tagtttttaa aaaagtgatg gaaacaccag ttaagtaccc tagaaaagct ggtaaaccag
    17761 ccaaaaatcc tattaagtaa tgaaaaattt attattggta agcttaatta tttctcagga
    17821 aataattaag cttactgtaa aaaaacaaaa ataaaggaat tattaaagta gatgcagaaa
    17881 taaatttgta tgtacatata gagaacaaaa ataatttata tctaaaataa gagaggtatg
    17941 ttatggaaga taaaagagtt atggagatac ctatagaaga tgtagttcca aatccatatc
    18001 aaccaagaaa aatattttca caagtttcat tggaggaatt gagcaattct ataaaggtat
    18061 atggtataat tcaacctata acagttagag caaaagatgg caaatatgaa ctaattgcag
    18121 gagaaagaag attaagagca gctaaattag cagagcttaa aaccatacca gcaattataa
    18181 ataatatgaa cgatgaatct tctgctgttc tagcgttatt agaaaatttg caacgagaag
    18241 acttaaattt cattgaagaa gcaattggtt atgaaaactt aataaaagaa catgctttta
    18301 ctcaacagca attagcagaa aaattgggca aaaatcaatc tactgtagca aataagctta
    18361 gaatattaaa gcttccaaac gatataaaaa tgaagctaat agagaataat ttaacagaaa
    18421 gacatgctag agcatttctt aaactaccaa gtgaagattt aatgcaaagt gtgttagaca
    18481 aagtcataaa aaatgaattg actgttaaaa aaacagaaaa attgatacaa gatgtattag
    18541 aagaaactaa agttcaagaa gagcctgata aaaaacagaa tataaagggt gctatgagta
    18601 taagaattta cataaatact ataaagcagg cctttgatgc tatatcaaat acaggaattg
    18661 atgctaagta taatgaaata gataagggag attatatgga agttgttgta aaaatcccta
    18721 aaaaatagag attactgctt accagtaatc tttttttgta atataagcct tgtaatataa
    18781 attgatttat agacattatt tatacttaaa aaacacctat tcaacattta aaattaattt
    18841 aattcttaaa ggggggaaca tcatgggcaa agttatagct gtattcaatc aaaagggtgg
    18901 agtcgggaaa actacaacaa atgtaaattt aagtgctagc ttaggaactt tgggaaagaa
    18961 aatattagta ttagatttag acccacaagg aaatacaact agtggatatg gtattaataa
    19021 aaacgaagta gaaaatacaa tctatgaaat tatgttagat gggttacata ttaaagaagc
    19081 aataataagt actgaatttg agaatgtaga tgtagttcca tcagctactg aactatcagg
    19141 tgcagagata gagcttacat ctaaaactaa tagagagtat attttaaaaa attccataaa
    19201 agcggtaata gatgaatatg attatatatt tttagattgt ccaccctcac tggggatgct
    19261 tacaataaat tgtttaactg ctgtagatag tgttttaatt ccaatacaat gtgaatatta
    19321 tgctttagaa ggtgttagtc aattaatgga gactataaaa ttagtaaaat caagattaaa
    19381 tgctgatata gaaattcaag gtgttgtttt aagtatgttt gatggaagag ctaatttatc
    19441 aatacaagta gttgaagaag ttaagaaata ctttaaaggt agtgtatata caactttaat
    19501 tccaagaaat gtaagacttg cagaagctcc aagtcatgga aaacctgtaa tttattatga
    19561 taaaagatgt agagggtctg tagcttattt ggaactagca gaagaattta tagatttaga
    19621 agaggaggaa tggtagtatg gagaataagt ctaaaagaag taatagatta ggtagaggtc
    19681 ttagtgcatt gataccagaa attaaaggag aaacttcaga aaaagaaata gtcaacatag
    19741 atatagacaa aatatatcca aacgaagttc aacctagaaa gcaatttgat gaagaaaaga
    19801 taaaagtatt gtcggattct ataaaaaatt atggtgtttt acagccaata gtggttaaaa
    19861 tggatgaaaa taataaatat atgataatag ctggagaaag aagatttaga gcatcaaaac
    19921 ttgcaaataa aaatcaaata cctgctataa ttaaagatat agatatgaaa gatataatgg
    19981 agatagcctt aattgaaaac ttacagaggg aagatttaaa ttcaatagaa gaggctctag
    20041 catataagag tttaatagag cattacaatg taactcagga agaaatatca gaagctgttg
    20101 ggaagagtag acctcatata actaatacac taagactttt aaatcttgga caagatgtta
    20161 tagaaatgat agacagtggt agaattacag caggccatgg taaggcttta ttgagaattg
    20221 cagataaaga tttgcaactt caaatagcga aaaaaataga agaagaagaa ctttctgtaa
    20281 gagaagtcga aaatatagct aaaaaaatat ctgaaaataa acaagaagaa cctaaaaaat
    20341 ctaaacctaa ggatgtattt attttagatg ttgaagataa acttagaaat atatttggaa
    20401 caaaggtgaa tatttctaaa ggaaagaaaa aaggtaaaat agagatagaa tactataatg
    20461 atgatgattt aaatagtatt gtatcaatgc ttcttgaata gaacaaaaat ataaaacaga
    20521 gaggaagatt gttatgattt atttagataa tgctgcaact acatatccaa aaccagaaag
    20581 agtgtataat gctgttttag attgtatgaa aaattattgt gcaaatccag gtagagcagg
    20641 ccataaactt gctatgagag ctgctaggga gatatatgat acaagagaga atatagctaa
    20701 gttgttcaat gtaagtaatc ctatgaatat agtatttaca tctaatgcaa cagattcttt
    20761 aaatttagcg ataaaaggtg tattacaaga aggagaccat gtaataacaa cgtctatgga
    20821 gcataattca gtaataagac ctataaaagc attggaaaaa aggggtattg aaaatactgt
    20881 agtaaaatgc gattatgagg gatttttaga ttatgaagat ttagaaaaat caataaagaa
    20941 taatactaaa ttgattgtaa caacacatgc gtctaatgta tgtggaactt taatagatat
    21001 aaaaaaagtt ggagaaatag ctaaaaaaca taatatacta tttttagtag atgcatcaca
    21061 aacagcaggt gtttatgata tagacgtaaa tgaatgtaat atagatatgt tggcaatgcc
    21121 aggtcataaa tgcttatttg gacctcaagg aacaggtata ttatatgtaa gagaagggtt
    21181 gaacttaaat atattaaaag aaggtggaac aggtagtaaa tcagaagaaa tagttcaacc
    21241 agagttattc ccagataaat atgaatcagg aactcataat actccaggta tagcaggtct
    21301 taaccaaggt atactattta tatttgaaag aggaatcaac aatataagac aacatgaaga
    21361 agaattatgt caatatatga tagacaaatt agaggaagta ccagatataa agatatatgg
    21421 accaaaggac agtaaaaaaa gagcatcagt aatagctcta aatataggag atatggattc
    21481 tggagaggtt actttcttat tagatagtga ttataatata gcgactagat caggaataca
    21541 ttgttcacca ctagcacata ccactttagg tactttaaaa caaggtgcag taaggttcag
    21601 tataggatat ttcaacacaa aagatgagat agataaagca gtggaagctt tgaaaaaaat
    21661 ttcaaaaaat aaatagcatg agatttaata atatagaaat aaaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggttc ctaagaacct
    21781 ctaatctatt tgataatagt ttcaagaaat tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 ggtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagagtaa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa atttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtatttat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttatg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattaaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaacca ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagaatct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaata taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagagcaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt atagggccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttga cttacattga ttttttatta agttaaagct
    34921 acttatacat attctaactg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 ataatattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taaggtacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag attctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aggtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcacttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacttt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tcttcattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag aaacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacataga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagctga aaacgttaga gattttgcat ttatactaag tagtaaattt
    38821 gatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagtga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcttt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aatagtgaat gtaagtaaat tttgtgcaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aaaaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttaa
    40261 gcaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aactgctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtggtaatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac cgttacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt taagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gagcttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctttagggct ttctgatgag gttttaggaa gggctgatta taggttatcc
    42961 ttttctaaga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gtttttgtta
    43081 atttgacact taataatatt aaaataacaa aaaagtattg aaagtatatt gcatttaagg
    43141 tagaattaaa taaaatatta taacattata atgtttatat ggttgttaat aaaaatttgg
    43201 aggaaaatat atgataaaaa taatgttggc ctgttcagca ggaatgtcta caagtttatt
    43261 agttactaaa atggaaagtg cagctaaaga aaatggtata gagtcgcaaa tttgggcaat
    43321 accagaatca actatacaaa atgaaataga aaaatgtgat gtattattac taggaccaca
    43381 agttagatat gtattaccta aggctcaaga aattgctaag ccatataata taccagtaga
    43441 agttataaat atgatgcact atggaacagt taatggagaa gctgttttaa atagagcgat
    43501 agaacttaac aacactaagt aatataaatt ataatgtatt tttaatatat aaattgggag
    43561 aatcaaagga tggatgaaaa gactatagaa atatctttta atataatagg ttatgcaggt
    43621 gaaggtaaag gattagcttt tgaagcaata aaagaggcta agaatggaaa tatagaaaaa
    43681 gcaaaagaat tattaaaaga gtccagggaa gttataaata aagcacatag atatcaaaca
    43741 gaattaatac aaaacgaagc tagtggaaac aggactgaaa taagtgtcat acttatacat
    43801 gcacaggacc acttaatgaa tggcatgaat tttcaacagt tagctgagga aatcgttgat
    43861 ttacatttga aattacaaga taaataaaaa ttaaagataa taaattaaga gagttcttaa
    43921 aagatacatt atgtgtagca cactaattgt tagattattg attcaatatt tagtgagtaa
    43981 tacattagga ttattttgag gactcttttt atatttagtt taaagttaat aaatatttat
    44041 tattaataat ataagattat atgttataat attataacat ataatttaag aggtgaatca
    44101 tgttaagaaa tcaaccatta tatatacaag ttgtagatat attaaagcaa aaaatattaa
    44161 ataacgaata taaaattgat gaaaatattc caacagaaag ggagttagag caagagtttg
    44221 gagtcagtaa aattactatt agaaaagcta ttgagatatt agaatcagaa gggtatgtag
    44281 agaaaaaaag tggaaaaggg acaaaagtaa taagtaatag tctttttaat aagctatcaa
    44341 aagcagagtc tttttcaagt atacttttat ctaaaggtca taagttatct aaaaagacta
    44401 tttcaattga aaaaataata aatgatacaa attcacaagt atttagtata tttggagaaa
    44461 aatgtagcaa gattactaga atgtattact tagatagtaa gccatatata ttttttactc
    44521 attatattcc ttttgatgaa aatctaactg ttgaaagttt taataatgaa agttctttat
    44581 atatgtactt atataggaga aatataaata taactagatt tgaagatgaa ttttttatag
    44641 aagaatcaga actagaggta tcaaaggctc taaatctagc taaagacata ttattaggaa
    44701 gaaaaagaaa gacctatgat gagaatgata atatagttga gctatcattt gcaagatata
    44761 atacagaagt acaaaactat gtaattaaat acgaaatata ataagaatcc tgagtttatt
    44821 gaaatgataa tatgctcagg atttttgttt agttgtaaaa ataaaaaata ttataacatt
    44881 ataatgttta caactaaaca ttataatgtt ataatatttt taataaacaa gtgatattat
    44941 caattacata aaaattagtt tatgttatga aatcgttatt gcattataaa taaaggataa
    45001 tatcaataat acagggggat aactatggaa aaattcgcaa gttttttaga gcgcaagatt
    45061 atgcctgtag cagcgaagat aagtaatcag agacatatga gagctgtaag agcaggtata
    45121 atagcaacat taccactaac aatagtgggg tcatttttca caatattatt aaacatacct
    45181 attgatgctt atatggattt tatagctcca tttaaagcaa tattagatat accatttaga
    45241 tatactgtag gatttttatc tctatatgct acttttggga tagcatcatc acttgctaaa
    45301 agctacgatt tagatacaac aggtgttggt atgcttgcag ttatggcatt tttagtttct
    45361 acaatagttc caactcaagt agttgaacca gtaggtggag ttatagaagc tggtagatgg
    45421 atggatatgg caaaacttaa ttcgccatca ttatttggag ctatcgtaac atcattaatt
    45481 acagttgaaa tatatcgttt tatgaaggaa aagaatatta caattaagat gccagcagga
    45541 gttccagtag aggtttcaaa ttcatttact gcattattac cgacagcagt aattttaatg
    45601 ttattctggg taataagaca tattttagga tttgatataa gtgttgcact aagcgctatg
    45661 ttatcacctt taaaaggatt cttatcagga aatagtttag gtggtgggtt actgacagta
    45721 ttcctaataa ctttattctg ggtacttgga atacatggtc cagctataat ggggccagta
    45781 ataagaccta tatgggacat gtctatagca gaaaatataa ctgcattcac agaaggagta
    45841 tcagctcatg caatgccaaa catatttaca gaacaatttt tacagtggta tgtatggata
    45901 gggggagcag gggctactct tgcattagtt gtaatgttta tgttttctaa gtcacaatat
    45961 ctaaaaagtt taggtagatt aggattttta ccagggttat tcaacataaa tgaacctata
    46021 atatttggag caccaatagt tatgaatcca atactaggga taccatttat aattggacct
    46081 ataataatga caattctatc atatgttctt actattacag gggttatacc tatgatggta
    46141 gcaagattac catttgcaat gccagcacca atagcagcag taatgagtac taactggagt
    46201 atattagcag gtatcttagt attaatcaac tttgtgatat catttgctgt ttattatcca
    46261 ttcttcaaag tttttgaaaa acaacaatta caaagagaac aagaagaaat gagtgaaaat
    46321 ttataaaaat acatagatat agaggttttg tactatataa aatatagaaa aaacttataa
    46381 gttattaagg gtgaaggtag gaggaatgac ctttgccttt aattttatat attttaaata
    46441 atgtaatttg aggaggagaa agatggaaag aagattagga atatcagtat atccagagca
    46501 tagtaacatg gaagatgata aggcatatat aaaaatggct tcaaaatacg gatttaaaag
    46561 aatatttatg tgcatgcttt caattacaag acctaaggaa gaagttaaaa aacattttaa
    46621 agagattata acctatgcaa gagaattagg atttgaagta atccttgata tagctccaaa
    46681 catatttgat atattacaaa tatcttatga tgatttaaca ttttttagtg agttaaatgc
    46741 tagtggaata aggcttgact atggatatga tggaagtaaa gaagctatga tttctttcaa
    46801 tccatatgat ttaaaagtgg aactaaatat gagtaatgat gtagcatact tggataatat
    46861 aattacttac aagccaaatg taaataatat atatggttgt cacaattttt atcctcaaag
    46921 gggtacaggc ttgccatatg atttttttgt aagatgttca aatagattta aaaagtatgg
    46981 aataagaact gctgcttttg ttaattcaaa atatgcaaca atcggacctt ggaatataaa
    47041 tgatggactg tgtacgcttg aaatgcatag aaacttagat attgctacag caactaagca
    47101 cttattcgca acaggattga ttgacgatgt aattgtaggg aatgcatatg caagtgaaga
    47161 tgaattaaaa agaatgtctg aagtaaataa atatcaagtt gtatttaatg tagtaactga
    47221 ggaaaaaact tctgaagtag aaaagacaat aatggcaaat gagcaacact ttagaagagg
    47281 ggatataacc aaccaagtag caaggtctac agaagtaaga aaaaaatatg ctaaagaaaa
    47341 tttccctaag catgataata cgaccatctt taaaagagga gatgtagtga ttggtaatga
    47401 tgactttgga aagtataaag gggaattaca gttgatactt caagattgtg cagatgaaag
    47461 aaaaaataag gtaggtagaa taaaggaaga cgaaataatg cttttagact ttattgaacc
    47521 ttggagtaaa tttaaactta ttttagaggg agagtaaagt gaagatagac atattaataa
    47581 aaggtggaaa aacagtagat aaaagtataa ttgatatagc tatattagac gataaaataa
    47641 tagaagtaaa aaatcagata gatgattcta agtatcaggc aaatcaaatt ataaacctag
    47701 atggagaaaa atttattagt gctggttgga tagatataca tacgcattgc tacgaaagac
    47761 tagaccttta taaggattat ccagatgagg ttggaataaa aagcggtgtt actactgtag
    47821 tagatgcagg aacaacagga gctttagata taggtaaatt ttatgaagat actaaaaaat
    47881 ataaaacaaa tgtatatgct ttaataaata ttgctaagca gggaataaca tctcaagatg
    47941 aactttcaag tatgatgaat attgatgaat atgaacttaa aagagcagtt aaaaaataca
    48001 aagattttgt tgttggaatc aaagctagaa tgagtaaaag tgttgtaatt tcaaatgatg
    48061 ttgaaccttt aaaagtagct aaaagaataa aaaatgaact taatctacct atgatggttc
    48121 actttgggag ttctcctcca acgatagagg atatatttga ctatatggaa aaaggagata
    48181 tattaaccca tatatataat ggaaagccaa atggaatact tagaggcaat gaggttaaaa
    48241 aagaaatcat agaagctaga gaaagaggta taatccttga tgtaggtcat ggaaccgaaa
    48301 gttttagtat ggatattgca atgaaatcta aggatgcagg aatttttcca gacactataa
    48361 gtacagatat atatattaaa aatagaataa atggtccagt gtataatcta tctacaacaa
    48421 tggaaaagtt tatatatatg ggctattcac ttgaagatat aatagataaa gttactaaaa
    48481 atgcagcaga tgcaataagt ttaaaaaata aaggtttaat aaaagaagga tatgatgctg
    48541 atttaactat atttgatgtg gttaatgaag aaaaagaact tcaagattct ttgaataagt
    48601 cagtaatcac aagtacgtct ataaagccaa gagcagtagt agtcaatgga gaatatttaa
    48661 atataggtaa gtctatagga gagaatgagt agaagggaga aactattgta tgagtagtat
    48721 atttgaaaaa tactctgtaa gacgtgttat aaatgcttca ggcaaaatga ctatactagg
    48781 agtatcaaca gtagataagg aagttattga agcaataggt gaagggtgta aaaatttctt
    48841 tataatagaa gaacttataa ataaaacagg tgaatatata gcaaatttat tagggtgtga
    48901 aagtgcacta atagtttcta gtgcatctgc tgggattgca cagagtgtgg gagcaatgat
    48961 agcaaaagat gatatgagtc tagtttacaa tgtaaataat cctaagaaac aagttaaaag
    49021 ggaaataata attccaaagg ggcataatgt tgattatgga gttccagtag aagtaatgat
    49081 aagacttgga ggaggagaag ttgttgaggc cggctatgct aatatgtgta acaaggaaca
    49141 tataataaga gagataaatg aaaatacagc agcaattatg tatattaaat ctcatcattg
    49201 tgttcaaaag agtatgctaa gtgtattaga tgcagtacaa gttgctaaag aatataattt
    49261 acctttgata gtagatgcag cagcagaaga agatttaaat gtatattatg aattgggagc
    49321 agacctagtg atatacagtg gtgctaaggc tatagagggt cctagctcag ggttagtaat
    49381 aggtaagaaa aaatacatag ataatgtaaa gcttcaatca aagggtatag gaagagctat
    49441 gaaaatagga aaagaaaata ttgtaggtct aacatgtgca attgagagat atataagtaa
    49501 tgataaagta actttaaaag aaatggagga caagctaaat ccatttatag acaagataaa
    49561 tactataaag ggagtgagtg ctagtataac tagagattca gcaggtagag aaatacttcg
    49621 tggagaaatt gactttaatg aagatataat aaataagtca acacaacaaa ttataaatga
    49681 acttagaagt ggagagatag ctatatacac tagagactat aaggctaatg aaggcaagat
    49741 tgagattgat ataagaagtg taactggaag tgatttagat actatttata ataggataaa
    49801 aaatatagta gaaagttaga gtgtagagta agtgacatta taatgattta ataaattaaa
    49861 ttttaccaat ggaggggata aatatgaatt gtataccaca atattataaa aatagagttt
    49921 gtttaaatgt tcttgcagga agcataaaaa atgctaaaga agtatatgat gcttgtgatg
    49981 ggtatgtatt gataggagta ctatcaaaga attacagtac tgtagaagca gctattgaag
    50041 atatgaaaaa atactcaaag gaaatagata atgcaatatc tgtggggctt ggagctggag
    50101 acccaaatca gtcaaatatg gtatctcaga tttctaaaga acttcagcca caacatataa
    50161 atcaagtgtt tacaggtgtg gctacgtcta gagcattgct tggtcaaaat gatagtataa
    50221 taaatggatt agtttctcca actggtaaac caggatttgt aaaaatatcc acaggtccat
    50281 taagttcaaa agaaaaagat ggtatagttc ctatagaaac agcaatagct atgatgaaag
    50341 atatgggagg aagctcaatt aagttttttc caatgggagg gcttaaaaca aaggatgaat
    50401 atatagaagt tgcaaaagct tgtgcaaagc ataattttta tttagaacca acagggggta
    50461 tagacttaga taacttcaaa gagatagttc aaattgcact tgatgctggt gtagaaaaag
    50521 ttatacctca tgtttataca tcaataattg ataaagaaac aggtgaaacg aaagtggaag
    50581 atataggagt actttataaa ataatgaaag aattattggg atagatttat gaatatatta
    50641 gcttttggtg aaataatgat gagactttca gtacctgact acaagttctt aacacaaacc
    50701 aatgaactaa attatataat tacaggcaca ggattaaata tattatctgg acttaaaaac
    50761 tttggataca atacctatat gttgacaaag ttgcctaata ataatgtagg taaagcatca
    50821 agtgcaaata taagaaagct tggagtaaaa gatgacttta tcacatatgg agggaatcat
    50881 ataggagtgt attttcttga aaatgggtat ggagaaagac cttctgaagt gacttatttg
    50941 aataggttaa atagttcatt ttgtgagagt aaattacaag attatgatat tgataaatgt
    51001 ttagaagaaa tggatatagt ccttatttgt ggtattgcta tgcaacttac agattcaatt
    51061 aaagagattg ctttagaact agctagaaaa tcttatgaaa aggggataaa agtattcttt
    51121 gattttaatt ttagacaatc attaaatcca gaaaggactt atgatgattt agtaaatgat
    51181 tataagaaaa tactaccata ttgtcatggt ctatttgcta gttatagaga tataaataag
    51241 atacttaata tagatagtct aaatataaaa tctagttcag aattgattaa aaaagagtat
    51301 gatataggta ttgtagctgg aactatgaga agatttgatg atatgaataa tagatttata
    51361 aagggatata tatcaaatga tgatggttat tttgaaagta gggagtataa gttagaaata
    51421 tatgacaggg ttggtgccgg agatggatat gtagcaggaa ttatatattc atatttggaa
    51481 aatatgaaga gtgaagatat agttgagttt ggggttgcta gttcggtgtt agctcataca
    51541 acttatgggg ataattcttt agtaagcaca ggtcatgtac ttagacttat gaacaatgaa
    51601 aaaatagata tattgagata atacatttgt atataataaa atttttatct tttttagaca
    51661 cacttttgac ttgtttatat ttgttaaaca gagttatttt taatgattat aaatattaaa
    51721 ttaaaagtta aataataaaa tagtagaaaa tagcattaac tatagaaaaa atggttaatg
    51781 ctatttttat cgttaatatt gattaatttt atattataga aatgaacttt tctaatgctg
    51841 gatttgtatt ttttttattc catacaacta caacttcaat tctattttct acttctttta
    51901 taggtactat tgcaatatca cgttctatca ttgtatctgt aacacaaaat gaaggtaaaa
    51961 tggatattcc catgttagaa gataccaatg attcaatagt attaagatta tttgaacgtt
    52021 gtaccacatt aggtacaaac cctgcttcac gatatctatt ctgtatcatt gacttttgtc
    52081 catactcatc ccctgtttct tttacaaaaa taaatttatc atgttgtaat tcaattaaat
    52141 cgatagaatc ctcattgtaa agtggatgac caggaggtaa aagagctacc aaagaatatc
    52201 tttctacagt tttatattca aaatctttta atggttgatt ttctggatta ataacaaaag
    52261 cgaggtccat agaattattt tttactaagt ttaaaagttc ggttatttca ttagtacaaa
    52321 tatccataga tatattaggg tatgtattag aaaattcttt taaatattgt tgaagtttgt
    52381 ttttttcata tcctattaag gttccaatgc tcaaagttcc aaaaaaacca tgtgcaaatt
    52441 gttgtgtttt tgcaatagca tcattgatat tttttattat agtacgtgcc tcagataaaa
    52501 aaacttttcc agctggtgtt agttcaacat gacgtttact tcttgtaaat agagttacat
    52561 ttatttgttc ttctaaagct tttatttgct gtgtaacagc agtttgagaa atataaaatt
    52621 tttctgctgt tttggtaaaa cttaaatttt ctgctacaga tataaaatat tctaattgtt
    52681 tagtattcaa ataatcaccc ctatttaaac ttgattgttt gtaaacctta tttatctata
    52741 attaaagctt attatagtat aagaaaattt acttttaaag aatattagaa aatatttgta
    52801 taaaaatata tgttttaagt atatctttac attagatttt ataaatttat taataagaat
    52861 aacttatata ttccaatgaa aacgatattg gatttaagta agtttgtctc ataaaatgaa
    52921 agtatatcaa ttgataatac tttaaataag aagggagaaa aaatggaaga taaattttat
    52981 gcaaaaggca acggaaataa cggatatatt aaaaatcttg aagtttgttc ctttaataac
    53041 ttagatggaa cttgtggaat gtttcaaatg gctctgtaca aaagagatga aaaatactat
    53101 ttatatggat gctgttttgg aggaaataaa aaaaatggag taatgattag cgatattaca
    53161 gacccttata atccacaatt tataaaacat tttcaaatgt tagaccctaa agagtatcct
    53221 acaacaacaa ctcccaaaat tcaaatagca gatgatttaa tgatagtagc aatgagttgt
    53281 ggaagtggac caggagcact tgttgaccaa gctaaattag caaatattaa gtgtgaagca
    53341 ggaattagaa tatatagttt aaaagaagac cctttaaatc ctaagttttt aggatattgg
    53401 gattgtggct taaagcatgt aatgggtgtt catagattca tgtacaatgg tggaagatat
    53461 gtacatttat caagtgattg tgttggcttt gaaggtctga tttatagggt catagatata
    53521 ataaatccta ctaatccagt ggaaataggt aaatggtgga gaccagacca atatgcagat
    53581 ggatatccaa atagaacttt tgatgcagga gcacctcatt gcccagaatt tatggataaa
    53641 ggatggcttc atggacctcc atttgtaaga gacggaaaag catattgtgg ttatggagga
    53701 gctggtttag ttgtattaga tgttgaagat ttaacaagac caagatgctt aggtgaattg
    53761 ccatttatgc ctgcattttc tagtagactt gcaggtgcaa gaactcatac agcattacca
    53821 ttgccaggaa gagatttagt cgttgttcaa aatgagggag aaagattcca gttctttaaa
    53881 ccagataaca ttacagatgt tcaagctatg aataatatac atatggttga tgttagtgac
    53941 ccaacaaaac caacattaat tgctcaattt ccatatcctg aagttccaaa agatttccct
    54001 tatcctaact ttaatgttgc aggattagga aaaccagggc catttggtcc acataatctt
    54061 catgaaccaa tggataataa accatggtta gagcaaagag gagatagagt atattgctgt
    54121 tatttccatg cagggctaag ggtttatgat gtatcagacc catattatat caaagagcta
    54181 gcatatttta taccaccaaa tccaaataaa acaccagaag aatcttattt cccaggattc
    54241 ccaggaccac gcttggcagt aacagaagat cttatcgttg atgatagagg ctacatcatc
    54301 atagatgctt tagatgatgg attctatata ttaaaaatga aagaagatta aataaatact
    54361 aaagagggag agttgaaaat gaaaatacta ggtatttctt ttggaacaaa aaatggaaat
    54421 aatgacacaa tgtgtaaagt tgcacttaaa ggtgccaaag aagcaggggc tgaagtagaa
    54481 tttattcaaa tgtcatctat taacattaaa cattgtactg gatgttgtgc gtgtgttaaa
    54541 actttattat ctgggaaagg tagtatgtgt gtattaaaag atgattttga atggttgctt
    54601 gacaaaatga aagatgcaga tggtatagta gtttcagacc ctatttttga agaaggggca
    54661 tcaggattat tccatactat aatggaccgt tttggaccca gagcagatag aggaaataac
    54721 attataggaa caaaagttgc agaagctata ggaggaaaaa ttccagattc aagaatgctt
    54781 aatgataaag taatatcatt tatgggaata ggaggttctg actggggtac aagagttcaa
    54841 tgtgaacatg ctatgctagc tctcatacca atgtggaaag ttatagataa tgcttggttc
    54901 ccatgggcaa aggaattagt aatggatgat acaaggcttg ttcaagttca tcaaattggt
    54961 ttaaatatag ttgaagcagc aaaagatttt gaaaaagctt catatcaagg agaagaaggt
    55021 gtatgtccac attgccacaa tagattattc taccttgagc caggtactaa aaaggcaatt
    55081 tgtgctctat gtggtatagt tggagagtta gatactataa gtggaaaaac tatatttagt
    55141 tttccagaag agcaattagg tcatgctcat gatactttac caggtaagtt tatacatggt
    55201 gatgatatta agaaaatgga aggtcattat gcagaagttc gtaaaacaca agaatttaaa
    55261 gaaagaaaaa catcttataa ttttatagag acattaacta aagaaaaaca agctggagtt
    55321 tcattaggat aataattaaa atactaagac tcttttttaa gggatgttag atgttatagg
    55381 aggcttattg tatgaatgaa aagccaagtt ttaaacaaat tgaagttatt ttagcagtta
    55441 tttttttggt acttacatat tacaaggtta gattaactcc agcatttatt gttatattta
    55501 taccactata tttaagttat tttgcagtat ctatgattgt aaatcaaata gcaattaagc
    55561 tattggagat aaggcaagct agggaagcta ctaattacaa tataggaaat ggatagaaag
    55621 aaaaaagaaa tattaacact gtctatgggg attgctttac tacctccttt atgggcagta
    55681 ttagcacctt atataggaat aaaaactgga gctgtagcac taatctgtgc aggattatat
    55741 gtaactaatg gtaataaaca agaagatgga ttaaaaataa tgtttggttt ttggtgtgga
    55801 gatttatggg cagtgttagc cattttaatt atgggatata tgaatttcaa tcaaaaccta
    55861 gaattatttt taacacttag tatattaggt ttttttgcag ttgtaattgc atccttattt
    55921 gaaaaaataa ttttcctacc atcatggctt tgtggttggg ctataggtct aactattatg
    55981 acaggtgaaa atattattaa tttacaagat atttgtattc aaattgctgt agctatggca
    56041 gtaggagtat ggtacgtggg tgcaggtgta gatttgttta caatatatat tttgagtaaa
    56101 gataggaaaa agggaaattg aaagcatgtg aatatacttt gattatagga ggatggctat
    56161 gggttttgaa tttaaaataa tgagaagttt aatatatgta ggacttgcca aggaagaata
    56221 tagacctaag ctaatggact ggttatatcg tcaccatatt ccagatagta ttagcacttt
    56281 tggaccatat tgtactaaat atgcctttta tcaagcatat cctacaccaa atgaaggtga
    56341 gcgttttggt gcacgtaaga tgcaactaac agaacattat tggcttgtag atgaacatat
    56401 gcctgagatg gcaaatagaa ttatgacaga atatatgcct atggatgttc tacgttggca
    56461 agggtgtata ccagatgtag aaaataaaag ggttcatgaa aatgcagaaa gtggagatgc
    56521 aggacgtgca gtaggtggag ataatggatg tccaccattt atatttgcct ttgttccaat
    56581 aaactgggaa gaagacttta gaggaaaagg acgtactgta caagatggac caaactatcg
    56641 ttggcaattt atgattaagt atccagatgg tatctctaaa gaagaaggag aaaaatggtt
    56701 ctatgatgag gtagtgccat actttacaaa ctgttgctat gttaatcgtt ttgtcagtag
    56761 taaaataatg attaattatg gagcaactgc ttttgaccgt gtgtcagaac tatggtttga
    56821 aggggaagaa gaatggtata aagctgtggt tgaagagaca aagtcgttta ttaaaaaacc
    56881 agaatgggca caagaagagg agttcccata tttaaaacca caattcaata tcgcatcagt
    56941 attcttaggt gatatagcaa ctatggatgc atactcacag tatcgtggat atataccaat
    57001 gagataaaaa ttatagtttg tgataagtag agaaggcatt tactcttact ggtagaagaa
    57061 gacttattgt atccttaaaa gtaggtgaat atatagtttt atatttaaat ttatataaaa
    57121 ctaagactaa taaatatgaa acaatagaat tagaataatt tgataatatt tgttaaaaga
    57181 gcatttagaa tgtgtttaaa gattaagata ttccttttat ggtagcagat agttgaaata
    57241 ataaaactgt tatcatgaaa ggaatatttt tatgtatagg aaatttttaa aaataaaagt
    57301 taaatatgtt taagaacatc ttaaagtgaa agaatcattg ttagaaatat gtagacaata
    57361 tagtatttat aacgtatcat attaacaagt ttacacttgg attatgaaat aaaaagagta
    57421 attctaaagg ttgtaaatac tatgctgact ttgatacttc aaagtttagg tgattttgta
    57481 attgttaata gaaatacgtt taatgagcat aaagaattta tctaataata ggagaatata
    57541 atgtatacaa ttggacaagt atctaaattt ttaggaatat ctagagatac actaaaattt
    57601 tatgaagaca aaggattagt aaatcctaag aaaaatgatg aaaatggata tagaatatat
    57661 aatcaggttg atatctatga tattgcaaca ataaactttt atagagaaat tgatatagaa
    57721 attaaaaaga ttcaagaaat aagaaaaagt aaaagtataa acaatttaga actcttacta
    57781 gaagagaagg aacaaatcat actaaaagag atagaatata aaaagcttct cttaaaaaag
    57841 gttcagttag tcaaagaaga ttgtgacaaa atcaaaacat attttggaaa atacacaata
    57901 aaagaaatga agcctttgga agtaaaaggt aaaataaaac atgttactaa ctatgaggaa
    57961 tatgaagcct taaaagagaa tacaaacaac ttaaaaaaag cagttacatt aactgattta
    58021 agaagagtaa taaattttaa tgaagaaggt ataatagaag aaaaatttat aattgtaagg
    58081 gaagtagatg actacaacaa aatcatagaa ggtgaagttc tatctcatcc caattgtatt
    58141 tatactatta ttgaagatgg cagatggtca actggaggag aaaatattga taataaagtg
    58201 gaagacagat taagagaaat agcaatagaa aatgaatgtg aattacttgg aatagtctat
    58261 gttaatatat tgcttactac ttatgaagaa gggattgagc gtatattttt agagatttat
    58321 gcacctataa aataatataa aagcattgac ctgggactta ctcataggtt taaactcatg
    58381 atataagcag ttataactta tatagcaatt aaatattaat ttgaaatgag gattacttat
    58441 gagaacagag aaatcattaa aaaggcctat agccagtttt attattctaa caaatataat
    58501 ttttttacca ctttttttat tggtagtagt gactataatg ttaaaacttc caacagtgat
    58561 atctgacatt gcgttgtgta tatcagcgtg gtcatccact tttgcattta tgatattatt
    58621 taaaaaaatt tatccaggta agaaattttt agtacatgta aaagatagat ttagaaataa
    58681 acttaaattt tcaacagtat ctataataat tagtatacaa gtcctaatag ctgtagtagt
    58741 tatatttata ttagcatcta aaaatcatgg aataatgcct aactttacag tttcatcatg
    58801 gggagcattt atatatttat ttttaaaaaa tttatttgca ggatcattag gtgaggaatt
    58861 aggttggaga ggatttgttc aaaatcatct tcagaaaaga cattctcctt taaaagcatc
    58921 cataacagta ggtttttggt gggggatgtg gcatttacca atatggttta caacaggatt
    58981 caaaggactt gatttaataa aatatattat attttttatg attgcaatta tatcaatcag
    59041 tattataatg acaacttttt ataatctaaa taaaactttg ataataccta ttgcaataca
    59101 tcaactattt aattttttaa tagggataat taatgaagat ttaatagagc ttataaatta
    59161 ctatgcaatg ttatatttaa tatttgcaat tgcattaata gttataaatc caggaaatgt
    59221 attatataaa aatattagag agtaatatat atctttatgg taagatataa agatattatt
    59281 agaagaacat ccaaggagga ataaacatgg ttttactgat tgttgatgca caaaagttaa
    59341 taacaaacga gaggttatat aaatttaatg aatttgtagt aaatgtagaa aatttaattg
    59401 atacagctag aaaaaataat atagaagtaa tatatgtacg tcatgatgat ggagttgaaa
    59461 atgaattgac taaaggtaaa aatggctttg aaatatacga aaaatttaaa ccatgtaatg
    59521 gagaaaaaat atttgataaa aaagtaaata gtgcttttaa agaaactggt ctattagaat
    59581 acctaacaaa taatggtgaa aaagatatta ttattgctgg tctccaaaca gattattgta
    59641 ttgatgccac tataaaatgt ggatttgaac atggatttaa tataatagtt ccagcttatt
    59701 caaatacaac tgttgacaat ttttttatgt cagcagaaca atcatataaa tactacaatg
    59761 aatttatatg gaatgaaaga tatgcgaagt gtatttcatt agaggaaaca cttagaaaaa
    59821 tgaaataaaa atagagtatg aattgtatat gtacataagt tgatttcata aaaaaggatg
    59881 ttaaattcca agttaaaaat tagattaaat atattatatt atagataagg cacatcaatt
    59941 ttattttaaa ttaatgtgct ttttaattgt agtaatttat ttataatcaa tatgattttt
    60001 attatttaaa tcaatttttc ttgagtttct ttgtctaaag aaactttttt tatttataaa
    60061 atctacagtt atattatagt atctatttta gatgagttta atttttgtat caatctaaaa
    60121 caaaagttat acttaaatga aattgaaaac gataaaattc tcaaataaaa taagtattgt
    60181 ggaggtgatg aaagaatcca atgataatat gaagactaat cttaataaga aagtaggggt
    60241 taatggtagt ggacaaagaa attgtatcaa ttctccagat tttattaaat agcacattga
    60301 ttacaactaa tggattacaa gaggagtcta attcatcaaa gagacaaatt acttatagaa
    60361 taaataaaat caatgacatg cttaaagtaa aaaatgtacc acttatatgt ttaagagccg
    60421 acaaagatat tatcgttaga aaagaaacaa aaaaagcaat taaagaaatt ttagaaaaaa
    60481 actattctaa gaatacttat tattttagta agacagaaag acttttatat atgtatttaa
    60541 tgctttttat taacatggag gatatttcaa ttaatcattt tataaattct ataaaagtaa
    60601 gtaggtctac tgtaaatctt gatttcaagg atttgatacc agagcttgaa aagaagaaca
    60661 ttaaagttaa gaacaatcgt ataaatggat attacatagt tggcaatgag atggaaatta
    60721 ggagagtact tattaaaaac attatagaaa ctttgtctaa tgagaaaagt tcaagagtat
    60781 ttgatatttt tattaaagaa tataatttag atagttttga agaggctaaa aaaattatat
    60841 taaagctaat aaaaaaatat gaaattactt ttgttgaaga tagattaata gaatttatat
    60901 atatatttat ctttctaaaa gcaaggatgt attcaactag aatagttact caggaaaatg
    60961 tagatattcc taatatttca gttatgagtt caatgaagga gtataaattt gctaaggaat
    61021 tattagaagt atatgggagt gaagataaga taaagcctca caatataatg tatattagtg
    61081 cttggatttt aggtatatca gtaggaaatt atgaagaaga tacagaagat aaggatgtaa
    61141 tttcaaaaat tgtaaataaa atgatgacaa agtttggata tttaagcgga gtttactata
    61201 taagccaaga aaaaatattt aaacaattat attctcattt tagacctgcc tactatagac
    61261 ttttatttaa attacctatc tataatccga tttgtgaaaa agtaaaagaa gaatataaat
    61321 tagtttatag aattgtaagt aatgcaatga aagagttttg cggattattt ggagaagaaa
    61381 ttcaggaaga agagttagca tatcttacaa tgcactttgc aacacttttt tcaaataaaa
    61441 aagaatttga tggttttaga aaaagaatag cattaatagt ttgttcaaat ggcgttggta
    61501 gttcagctat tttacatgca gaacttacaa acttatttcc tgacttacac tttataccaa
    61561 tgtcggaatc agttggatta gaaaatataa gtgaacctgt agatattgta tttactacta
    61621 attataacgc agagggttta aaaacagatg taccagtaat taaagtaagc cctgttatga
    61681 ccacaaaaga aaaatataag attacacgtg aagtgtatat tcaattaggt gatatttttt
    61741 taagacaacc taaaatagat gaggttatgg atataataaa aaaatatgca aatgtaacct
    61801 ctgaaagtat tctttcaagt gagctattag cttattttac acagatagag aattttacat
    61861 ctaaggaagg agaggggcct atgctaagtg agattactaa tgaaacacta gtaaaaataa
    61921 aggtaaaagc taagaattgg gaggaagcta ttagacagtc agcttctgta ttagttgaaa
    61981 ataacaaagt aactgaaggg tatgtagatg ctatgattaa ctcagcaaaa gcatctggac
    62041 catacatagt aattacaaaa catgttgcac ttccacatgc tagaccagaa gctgggtcaa
    62101 aagagattgc aataggcatt gctactttag atagtcctat tgagtttggg aataacgaaa
    62161 acgaccctgt aaaatatgta ttttgtctaa gtgcaataga caataatagc catttacgtg
    62221 ctatgtctga gctagttgaa ctattggaag aagatgagtt ttttaaggtt ttagatagtg
    62281 ctaaagaagc taaagaaatt attgatttta taaaaacaca cgaattataa aggaggattt
    62341 aattatgtat aaaattttaa tcgcatgtag agcaggagtt ggctcaagtt taatgttaaa
    62401 aataaagact caacaaatta ttaaggaaaa taactttcca attgaagtag agcatggttc
    62461 attggattca ctaaatggat tcaatggaga tgctgtagaa acattaattg atgttgctga
    62521 agaattaaaa aataaaaatt tgaagtttga tataattggg attcacaata ttgttgaccg
    62581 taatgaaata aagacagcac tagaaaaatt ccttgcttca aaagagggat aaatttaaga
    62641 tgataaggag gaatatttat ggagtttatt attagtttgc tgagtacacc tgctgttttg
    62701 cttggcgtag ttgcatgtat aggattgatt cttcaaaaga aatcagctgt tgaagtattt
    62761 acaggctctt caaaaacact tatcggtttt ttgatatttg gtattggtgc ctctgctatg
    62821 acaggagcac ttcaaaattt caataaactg ttccaacatg gctttggaat tactggtgtt
    62881 atagcttcac cagaagcagc tacagcactt gcacaaggta catatggttt tgcagtttca
    62941 tgtacattaa ttcttggatt catattaaat ttagtatttg cacgtattac aaagtttaag
    63001 aatattttct ttacaacagg tcatagtttg ttcttttctt gtgtattagt tttaattatg
    63061 aaagcacatg gatttgataa tacaatcact attttaatag gtggaactat cttagggttt
    63121 atgtctgctg cactacctca attctgtcaa ccatttatgc gtgaattaac aggtggcgac
    63181 gaacaagcta ttggtcactt taacatgatt ggttatggtt tatcaggtta tattgggcgt
    63241 ttattttcaa aacataaaga tgatacaaca gagactatag aatttcctaa gtggttatca
    63301 atattcagag acttcttaat gggtctatcc atagttatgt taatcttatt ttatattgca
    63361 acattaaaag ctggtcaagc atttactcaa gaaattgctg gttcaacaca ttggttggtt
    63421 tttccgctta tagaggcatt tacatttgtg gcaggtatgt caatattgat gtcaggtgtt
    63481 cgtatgttct tagctgaaat aactgcagca tttgtaatca tttcagaaaa gtatattcca
    63541 ggttcacgtc ctgcgctaga tgttccaaca gtatttccat atgcaccaaa tgcagttatt
    63601 attgggttta tatcagcata tgctgctggt ctattagcaa ttgctattat ggcatcattt
    63661 cctagtatat ttcctgtagt aattattcca gctgctcaca tatgtttctt ctcaggaggt
    63721 acagctgcaa tatttggcaa tacttcagga ggatggagag gggctattgc tgggtcattt
    63781 gttgtggggt tattacttgc atttttacca gttatactat atccagttta tggcactctt
    63841 ggtatagaag gagcaacttt ccctaatatt gattacaatg tagttggttc gatattacat
    63901 aatattttac aatttataaa acctatttaa actaaattat ataaaagtat agtcatagtt
    63961 actatgattt caaggaggat acatcttatg gaaaatacat tatctgaatt tttagataat
    64021 atatctaatg aattgtcaga atttattgcc tcaatagatg agacatctat aaataaagca
    64081 tgcgaattaa ttcttgaagc tgaaaaaaat cacggacgtg tacatgtgac tggtattggc
    64141 aaaccaggac atgtatcggg atatatttca tcattgcttt catctactgg aacgtcagca
    64201 tatatattgc atggtacaga agcagtacat gggtcaagtg gtcaagtagt tgaaggagat
    64261 gttgttattg caatatcaaa tagtggtgaa actcaagagt taaaagcaac tcttaagact
    64321 ctaaaagtaa atggagcaaa gataattggt gtaagtggta atgagagttc atttttaaaa
    64381 aatatttctg atatattcct gtttgcaggt gttaaacagg aaggagattg tcttaataaa
    64441 gctcctcgtg cttcaatttt agctgaaaca ataatattac aatcattaag tgttgtatta
    64501 caatatgcta aaggtcttaa tacacagcag tatttgaagt ggcatccagc aggaagtctt
    64561 ggaaagtcta ttagagaggg aatataattt tatgaaatat aaagggatta tatgtgcaat
    64621 gataacacca tttgatgaaa atcaaaatat taattcacaa gcaacatgtc aattaattga
    64681 ttatttgatt gaaaagggaa tttatggatt atttattttg ggcactaatg gtgaatgtca
    64741 tgtattaaca gatgatgaaa aagttgaatt tgcaaagatt gttattaatc atacaaataa
    64801 tcgtgtacct gtttttgttg gaacaggggg taattcaact cgtgaagttg tagatttatc
    64861 taagagaatg gaagcgattg gagcaagtgc attatctatt attacaccat actttgttac
    64921 accaacacaa caggaattaa tcttacatta caaaactatt gctaattcag ttaatttacc
    64981 tattataatg tataatatgc caagtaagac tggaattaat attgaaccag agtcagtacg
    65041 tgaattatca aagataaaga atattgttgg aattaaggat agtagtggta aacttgacaa
    65101 catgaaagct tatattgaag caacgaagaa tgagaatttt agtgtgtttt caggctcaga
    65161 ttcacttatt ctggatagtc taaaagctgg tagtcaagga gctgttgcag caacaacaaa
    65221 cttcctcaca gagattggta tctctatcta tgaaaacttt atgaaagggg atttggaaga
    65281 agctcaaaaa gctcaaagta gtatagaaga acttcgacgt atactgaaac ttggaacaat
    65341 tccatctgtt atgaagaaaa ctattgtctt gaatggaatt aatgttggaa ctgcacgact
    65401 tcctgtaact gaaccaactg gggaagtact tgaagaaatt aaacgagtag ttgaaaatta
    65461 taggacaata cttaataaaa gtagtgagaa tagataatat aaatagggaa attacactgg
    65521 tatataatta atatatatat tataaaacat gactaaaatt aatgaataag taaagtggtt
    65581 tgatgagtat agaggtgata atatgaaaaa gatgaatata accaaaagaa tgtcagaatg
    65641 tggggctctt gcaattgtaa gagaagaaaa tcttaaccgt gcatgtgaaa ttgctgaagg
    65701 atgtataaaa ggtggtataa cagttataga aatgagttat accctaaata atgctggaga
    65761 aataattcaa gggcttaata aaaagtatgg tgaaacactt tgtgtgggag caggaacagt
    65821 tttggatagt gaaacagctc gtcatgctat attacatgga gctcaattta ttattgcacc
    65881 aaattacaat gaaggtgtag cgaagatttg caatagatat cagattcctt atgctccagg
    65941 atgtacatca ttgactgagg cagtagacgc actaagttta ggagcaactt ttataaaagc
    66001 tttcccaata tcagatttct atggacctaa actagtaaag gtatttaaga caccaatacc
    66061 tgatatgcca gttttagcaa gtggagggat taatattgaa aacctacata tatggttaga
    66121 aaatggagtt gatgtatgtg gatttggtgg tttattaact aaaggttctg ttgaagatat
    66181 tgctaaaaat gctaaaaaga ttcgtgaaat cattaaaaat ataagaaaaa tttaacatag
    66241 ggctgtatca tagtatctta taattactat gatacagcct ttttaatatt attcaaatta
    66301 aaaacagttt acatttctat tatattacat ataatttcag taattttatc ttaatttttc
    66361 tatctggaaa aaaatgattt aaaacttaag ttatgatata atttcattaa aagaaggcga
    66421 tttttagtaa atatgagaaa ataatgtgtt tttaggggaa aagttaaata cagatttaag
    66481 tgaacaagaa tcgataatta agatattaaa tggtgaatag ggcatcaaaa aaattactaa
    66541 attaaaagtt ttagagggag aatcttagca agtgtgtgtg aaaattcaat ttcaaacaaa
    66601 aaataattgt aaatgaaatc aatttataca cgttgaagat tcacttttat cattatatta
    66661 aaaagcagag tgaattaaaa gtgagtctaa agataggagg ataattaaaa caatgaaaga
    66721 agacattagg ttacatcgaa tgtgtaaaaa agaacaagca acaaagaatg aagttatatc
    66781 agaggatatt ctaaaatcta tgagtattgg ggtcagtaaa cattttttag atgattcatt
    66841 tacaataata tggggaaata catcattcta tcaattatta ggatatacag aagaagaatt
    66901 tttagttaaa ttttctagtt taaaagatta ttatgaacag aatctttatc attttgaatc
    66961 tatgaaaaat cattttttag aggcttatca tcatggcgaa aagagtatag aatatcaaat
    67021 ttgcatttca gcaaataatg gagaccctgt ttgggtaata atgactggta tattaattga
    67081 atcacaagat aatgagaaat caatggtcta tatattgtat tcaaaaatta atgagttaaa
    67141 aataaagcaa gaagaactat ctgatatgga aaaagagagt acagagaatt ttaaatggat
    67201 gatgtcagag tatgctggga atgtttatat tagcgatatg gatacatatg aattactata
    67261 cttaaataag cactcatgca atactttaca ggcatcagca aatgaactta ttggaagaaa
    67321 atgttatgag gtaattcaag gtagaacttc gccatgtccg ttttgtacaa attcgtattt
    67381 aaaaaaagat gacatatatg aatgggaatt ttataataaa aaactgaaac gaacatttat
    67441 aattaaagac cgtatgttga attggcaagg gcgaagagtt cgtattgaat tatcttatga
    67501 tatgtatagt gctgaatata aattagcaaa aaaagatcag gaaagagaat caattttgaa
    67561 aactattcca gcaggaatgg ttagattaga tgcaagagat tatcttacta tactttggtg
    67621 taatgatata tttttgaata tgattgaata tacaaaggaa cagtttgcag aggaacttaa
    67681 taatcaatgt ggatatttat tttctgaaga ttataaacgt agtaagaaac tggcacaaga
    67741 tttgaaggag tctggggata atgttgtatt tgaagcaaaa atatatacac gttcaaggga
    67801 agaacgtata tggactgtta cattgtgtta tatcagtggg gaagacagtt gggatggaat
    67861 accatctttt tatagtatag gtattgacat aacaaaggaa agaaaacaga ttgagaaatt
    67921 acagcatata tcagagaaag atgcattaac aggaatttat aatcgtgctg agactgaaag
    67981 gcagattaag aaatattttg aaaaaaattt aaatgttatg ggtgcactgt ttatgattga
    68041 tacagataat tttaaacaga ttaatgatac agaagggcat atgattggag atatagtact
    68101 tactgagatg gcatcaggta tgaaaaaaat aatgcgtgac agtgatgttg ttggaagaat
    68161 aggtggagat gagtttacaa tttttatgaa gaatatttcc tcagttaaag atgcagaaaa
    68221 gaaagcagaa gaactattgt atatgttccg tcatctattt cagaaagaaa aaagttttct
    68281 aaaagtaact tgtagcatag gaatagctat atatccaaag gatggtacta cattcaagga
    68341 aatatatgca agagctgata aagccctgta tcaagcaaaa aatatgggaa aaaataatta
    68401 tgtaatctac aaccaagatt attttaaaga attagaagaa tttgattgtt cttctcttgg
    68461 tacagttatt gattcagaaa aacaatacgc tgaatatcct gataatctta cacgttatat
    68521 attccgtatg ctttatcaga ctgatgatat agaccaggcg attaatatga tacttgaggt
    68581 tgttgggaaa cagtttgatg taagtcgtgt ttatattttt gaaaatacag aagatggtag
    68641 atatacttct aatacttatg aatggtgtaa tgaaggaatc tcttctcaaa tggcttattt
    68701 gcaaaacagc aattatcaag actatgagga ttatgaaaag ctttttgagg atgaattagt
    68761 tttttattgt cgagatatcc atacattaag tcctaaacta gaagaattgt tttctagtca
    68821 aggtatccac tctacacttc aatgtgcata taaagaagat agagttttca gtggatttgt
    68881 tggatttgat gaatgcacag gactacgtct atggtcacaa gaggaaataa gtacattatc
    68941 attgatttct caaattatat caatgttttt acaacgtaaa aaaaataata aacttaatca
    69001 acagattcaa caataccaga atattttaga taatattgac cagtgtattt gtgtgattaa
    69061 taaagaaaat aattttttat tatatgtaaa taaaaaattt aaaaatacat attctaaatt
    69121 ccaaattgga caaccttatt atcttgattc tatagatatt cataagatat ccattatatg
    69181 gaataaaaaa gaagcatacc tatgtactag cacgatttaa tggtaaagta aagtttatat
    69241 tggacttaaa ttaaatttcg ttttaaataa gatattattt tagttgctta ctgaaaagac
    69301 tagaatctat attaatgaat tatactaatt ttataccact attaaaatat tgtttgctaa
    69361 aaataaaaaa tagccttaaa acagttgatt ataaaaaatt aactgcttta aggctatttt
    69421 tatctttgtg acgaaataga cacttgccat ttagaatgtg acaatatagt agttgtttag
    69481 aaaaggcaca aagcattata ctttaactaa agtaaaatgt attttgcttt tagaataaaa
    69541 acaattagga gggagctata attgaaaaag ataatatatg tattatttac aattgaacta
    69601 ttaatagtat ctttattagg tctaaatata gttaaagata atgaaattaa taatatttta
    69661 tataacgaga caacaagtat atctgtaatg tttaaagatt ataaaaagtt aaataaaaat
    69721 tatagtaagt ggattaaaga tattgcagat gaaaataatg tgactatatc aaagtatgtt
    69781 tttaataata atgaaaaatt atctatatat acgactgata caagtttgaa taacaatata
    69841 aagttaaaat caggaaaatt tcctaatact aattcaaatg aatttattag taatataaac
    69901 actaataata gtaatcaagt aggtaaattc tcaactatga gtaaagacat atcaatttta
    69961 attagggatt ttggtaaaat aagtgaagtt ggagaatcag gaatcttata tatttcaact
    70021 caaaatgaag atacaataaa taaaatatta agagaactaa atgtagacag aagtattttt
    70081 gtagcaagat tacatgatag atatgttaac tcaaatttat acttaaatcc ttcaataatt
    70141 agagatttaa tactaattat cctttgtttc ttggctacaa taatacatta ttcaattagt
    70201 gtgagtagag aggcttctat tttaagatta aatggatact ctaagctaga tataattgct
    70261 agagttataa aaaatatact tagaattatg attttatcat ctgtcacagc attattaata
    70321 tatataatct atattttaaa attaaatata ggtgttagag catgcttata ctttgctatg
    70381 ttttcgatta tatgtatttt gataaatatt ttgatatcta tattaatagt tggttttaat
    70441 agtagaagtt ctaaatatgt gctatctcta aatggaaaaa aatcttatgg ttttgtaaat
    70501 ataatgcatt tcacattgaa aattgtattt gtatcatttc ttatattatc cataaataac
    70561 tgtattttaa attataatat gctacaaact cagttaggaa atctatctga gtgggataaa
    70621 gcaaaaaatg tttataatct tactcttaaa gatacaggcg aacaatcttt aggaaaggaa
    70681 gaagtaatta aaaatgccaa gattaaaggt ttctataaaa atttggtaga agaaaaagat
    70741 gctttcttaa tagattcaac taattttgaa aaacttgaag atggaagtta catgtatgaa
    70801 ataaattcaa agggtaaaaa cccagaagta agtcaatatg gtaaaaatat aaaaataaat
    70861 aaaaactatc ttaaagttaa ccctatttac tcaaatggca aagaaatatt taatctaatc
    70921 gattatgacg ataagactat aaatttatta gttcctaaaa agttacaagt acatgaaaat
    70981 gagattaaaa aagagtttag agaattattt tattttgaaa aaatagaagt agaaaatatg
    71041 tacaataaag agttaaatag agcgttaaac aaaacaaaga agtcagattt aaatgttaat
    71101 ataatatatg tagataataa tcaaagctat tttacttata actcccttgt tatggatgac
    71161 aataggaatc tcattgatga cccaatagca atagttgaca tagataatat tgatgattct
    71221 ttttatcttt cttatatatc tagatgtgta tattttaatt ctaagaaatt agatgccctt
    71281 gcagatataa gtagcattat tgaagctcaa ggtgtagaag cgcatatcca aagtttacat
    71341 gctgtttata atgagtatgg tttagaaata aataaactag aaaaatattt gaatagtgaa
    71401 atttttacta taataataat agcaatatca aacttaatga ttacctacaa tatagtagct
    71461 agctactatg aaagaaataa atataagtta tatattaaaa aaatatttgg ttattcaact
    71521 acagctagaa gtatgttgtt aatagtctca ttaatattaa caaacattat tccaataggc
    71581 attatatcaa caagagtaga tttaagtaat aatattatct tatttggact tctaatatta
    71641 gttatagaag taattgtatc tattgtatta gacaagatta tctcaaatag ttcatttaat
    71701 aaaattataa aaggagaaca ttaaaaagat gatttcatta ataaatatat ctaaaaaatt
    71761 taaagataaa tctataatag acaaatttaa cctagaaatt tctaagggcg agtttgtagc
    71821 gataacagga aatagtggtt caggtaagac aactttactt aatataatgg gattattaga
    71881 aaaacctaat tctggtgata ttataataaa taatataaaa aatccaaata gtaaacagat
    71941 aagaatttta caaagggatt tttatggcta tctatttcaa aattatgctt taattgagaa
    72001 tgaaaatgtt gaaaataact tgaaaattgc tttaaagtat caaaaaaata tcaaccataa
    72061 taaaaaaata aattacgcac ttgagtctgt aggtcttaga aaatacaata aaaaaaagat
    72121 atacgaattg agtggaggag aacaacaaag ggtagcatta gcaagagtaa tattaaaaga
    72181 atgtgaagta atatttgcag atgaacctac aggtaattta gataaaaaaa atagagacat
    72241 agtatttgat atacttaaaa gtctaaataa gaatggtaaa actatcgtat ttgtaactca
    72301 tgatttagag ttatcggagc aggcagatag agttataaaa ttgtaataat atcaactata
    72361 ataaatataa gaaagagatt acagctaaat caagtttaat aataatacga atagaattta
    72421 aacattaagt aaattagtag tttgtaaata taagtataat atgatatcat aaaggctcaa
    72481 aagaaatttt atataataag aaatctaaaa ataacaaatg gaggacatat aaagatggaa
    72541 aatttaccaa attgcccgaa atgtaattca gaatatactt atgaagatgg aactctttta
    72601 gtttgtccag aatgctctca tgagtgggca ttaggattgg gagctgaaga agattcaaat
    72661 attatcaaag atgtaaatgg aaatgtatta aatgatggag attctgttac agtaattaga
    72721 gaccttaaag taaaaggaag ttcatcatct ataaagattg gaacaaaagt gaaaaatatc
    72781 cgtttaatcc atgactcatc agatggacat gatatagaat gtaagataga tggttttgga
    72841 gctatgaagc taaagtcttc tgttgttaaa aaagcgtaat attataaata agctgtagtg
    72901 tttattgttt ttcagtacag tataaaacca taagaatcta ttaagtaata aaagtttgtt
    72961 tatttatctt tatattttct tatggtttta ttaaatataa attttattga acaccaaaat
    73021 attctctcct ctacaaataa gccaatctta tttaaattaa tatactttgt actatttata
    73081 ctgaaattgt aaatttaaat
//
LOCUS       DAMJWU010000007        66441 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMJWU010000007 DAMJWU010000000
VERSION     DAMJWU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 66441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.007"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            89..1285
                     /locus_tag="PC460_000631"
     CDS             89..1285
                     /locus_tag="PC460_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF2599533.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1378..1902
                     /locus_tag="PC460_000632"
     CDS             1378..1902
                     /locus_tag="PC460_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF2599534.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1927..2103
                     /gene="rpmF"
                     /locus_tag="PC460_000633"
     CDS             1927..2103
                     /gene="rpmF"
                     /locus_tag="PC460_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF2599535.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2310..2867
                     /gene="fapR"
                     /locus_tag="PC460_000634"
     CDS             2310..2867
                     /gene="fapR"
                     /locus_tag="PC460_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF2599536.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2878..3900
                     /gene="plsX"
                     /locus_tag="PC460_000635"
     CDS             2878..3900
                     /gene="plsX"
                     /locus_tag="PC460_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF2599537.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3926..4912
                     /locus_tag="PC460_000636"
     CDS             3926..4912
                     /locus_tag="PC460_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF2599538.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5021..5950
                     /gene="fabK"
                     /locus_tag="PC460_000637"
     CDS             5021..5950
                     /gene="fabK"
                     /locus_tag="PC460_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF2599539.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5966..6916
                     /gene="fabD"
                     /locus_tag="PC460_000638"
     CDS             5966..6916
                     /gene="fabD"
                     /locus_tag="PC460_000638"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF2599540.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQVDLKYSACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQDTVPTGVGAMAAILGLDRSILENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLREAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6916..7665
                     /gene="fabG"
                     /locus_tag="PC460_000639"
     CDS             6916..7665
                     /gene="fabG"
                     /locus_tag="PC460_000639"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF2599541.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7719..7943
                     /gene="acpP"
                     /locus_tag="PC460_000640"
     CDS             7719..7943
                     /gene="acpP"
                     /locus_tag="PC460_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2599542.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7972..9210
                     /gene="fabF"
                     /locus_tag="PC460_000641"
     CDS             7972..9210
                     /gene="fabF"
                     /locus_tag="PC460_000641"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF2599543.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9436..11154
                     /locus_tag="PC460_000642"
     CDS             9436..11154
                     /locus_tag="PC460_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2599544.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKHDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11185..13185
                     /locus_tag="PC460_000643"
     CDS             11185..13185
                     /locus_tag="PC460_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2599545.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSELSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13536..13781
                     /locus_tag="PC460_000644"
     CDS             13536..13781
                     /locus_tag="PC460_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2599546.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13816..14541
                     /locus_tag="PC460_000645"
     CDS             13816..14541
                     /locus_tag="PC460_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2599547.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14563..15876
                     /locus_tag="PC460_000646"
     CDS             14563..15876
                     /locus_tag="PC460_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2599548.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVVMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYDENLKKIV"
     gene            16082..16621
                     /locus_tag="PC460_000647"
     CDS             16082..16621
                     /locus_tag="PC460_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2599549.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16710..18695
                     /locus_tag="PC460_000648"
     CDS             16710..18695
                     /locus_tag="PC460_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDF2599550.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18919..19887
                     /gene="spoIIIAA"
                     /locus_tag="PC460_000649"
     CDS             18919..19887
                     /gene="spoIIIAA"
                     /locus_tag="PC460_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF2599551.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19877..20398
                     /locus_tag="PC460_000650"
     CDS             19877..20398
                     /locus_tag="PC460_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF2599552.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20415..20612
                     /gene="spoIIIAC"
                     /locus_tag="PC460_000651"
     CDS             20415..20612
                     /gene="spoIIIAC"
                     /locus_tag="PC460_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF2599553.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20628..21014
                     /gene="spoIIIAD"
                     /locus_tag="PC460_000652"
     CDS             20628..21014
                     /gene="spoIIIAD"
                     /locus_tag="PC460_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF2599554.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21099..22304
                     /gene="spoIIIAE"
                     /locus_tag="PC460_000653"
     CDS             21099..22304
                     /gene="spoIIIAE"
                     /locus_tag="PC460_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF2599555.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22364..22981
                     /locus_tag="PC460_000654"
     CDS             22364..22981
                     /locus_tag="PC460_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF2599556.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22993..23547
                     /locus_tag="PC460_000655"
     CDS             22993..23547
                     /locus_tag="PC460_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF2599557.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23574..24263
                     /locus_tag="PC460_000656"
     CDS             23574..24263
                     /locus_tag="PC460_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF2599558.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSYSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24373..24735
                     /locus_tag="PC460_000657"
     CDS             24373..24735
                     /locus_tag="PC460_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2599559.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24820..25326
                     /gene="nusB"
                     /locus_tag="PC460_000658"
     CDS             24820..25326
                     /gene="nusB"
                     /locus_tag="PC460_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF2599560.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25310..26380
                     /locus_tag="PC460_000659"
     CDS             25310..26380
                     /locus_tag="PC460_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF2599561.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26377..27582
                     /locus_tag="PC460_000660"
     CDS             26377..27582
                     /locus_tag="PC460_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF2599562.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27584..27799
                     /gene="xseB"
                     /locus_tag="PC460_000661"
     CDS             27584..27799
                     /gene="xseB"
                     /locus_tag="PC460_000661"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF2599563.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27802..28689
                     /locus_tag="PC460_000662"
     CDS             27802..28689
                     /locus_tag="PC460_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF2599564.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28719..29156
                     /locus_tag="PC460_000663"
     CDS             28719..29156
                     /locus_tag="PC460_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF2599565.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29190..31055
                     /locus_tag="PC460_000664"
     CDS             29190..31055
                     /locus_tag="PC460_000664"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF2599566.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31188..32000
                     /locus_tag="PC460_000665"
     CDS             31188..32000
                     /locus_tag="PC460_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HDF2599567.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32020..33699
                     /gene="recN"
                     /locus_tag="PC460_000666"
     CDS             32020..33699
                     /gene="recN"
                     /locus_tag="PC460_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF2599568.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33914..34780
                     /locus_tag="PC460_000667"
     CDS             33914..34780
                     /locus_tag="PC460_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599569.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDIDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34873..35364)
                     /locus_tag="PC460_000668"
     CDS             complement(34873..35364)
                     /locus_tag="PC460_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2599570.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35425..35865)
                     /locus_tag="PC460_000669"
     CDS             complement(35425..35865)
                     /locus_tag="PC460_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2599571.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36026..37084
                     /locus_tag="PC460_000670"
     CDS             36026..37084
                     /locus_tag="PC460_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2599572.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGGAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37226..38050
                     /gene="spo0A"
                     /locus_tag="PC460_000671"
     CDS             37226..38050
                     /gene="spo0A"
                     /locus_tag="PC460_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF2599573.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38463..39584
                     /locus_tag="PC460_000672"
     CDS             38463..39584
                     /locus_tag="PC460_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599574.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39602..40477
                     /locus_tag="PC460_000673"
     CDS             39602..40477
                     /locus_tag="PC460_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF2599575.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40501..41193
                     /locus_tag="PC460_000674"
     CDS             40501..41193
                     /locus_tag="PC460_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2599576.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41201..42025
                     /locus_tag="PC460_000675"
     CDS             41201..42025
                     /locus_tag="PC460_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2599577.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42030..43097
                     /locus_tag="PC460_000676"
     CDS             42030..43097
                     /locus_tag="PC460_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF2599578.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43099..43635
                     /locus_tag="PC460_000677"
     CDS             43099..43635
                     /locus_tag="PC460_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2599579.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43703..44308
                     /locus_tag="PC460_000678"
     CDS             43703..44308
                     /locus_tag="PC460_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF2599580.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44320..45234
                     /locus_tag="PC460_000679"
     CDS             44320..45234
                     /locus_tag="PC460_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2599581.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45276..46448
                     /locus_tag="PC460_000680"
     CDS             45276..46448
                     /locus_tag="PC460_000680"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF2599582.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46495..47307
                     /locus_tag="PC460_000681"
     CDS             46495..47307
                     /locus_tag="PC460_000681"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF2599583.1"
                     /translation="MFEKIAQSSKFINSKSDIKPKIGLILGSGLGDLANDIEDPVAIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47511..48836
                     /locus_tag="PC460_000682"
     CDS             47511..48836
                     /locus_tag="PC460_000682"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF2599584.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49049..50128
                     /gene="mltG"
                     /locus_tag="PC460_000683"
     CDS             49049..50128
                     /gene="mltG"
                     /locus_tag="PC460_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF2599585.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50300..50974
                     /locus_tag="PC460_000684"
     CDS             50300..50974
                     /locus_tag="PC460_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF2599586.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            50961..52208
                     /locus_tag="PC460_000685"
     CDS             50961..52208
                     /locus_tag="PC460_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2599587.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52280..53944
                     /locus_tag="PC460_000686"
     CDS             52280..53944
                     /locus_tag="PC460_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF2599588.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54041..54325)
                     /locus_tag="PC460_000687"
     CDS             complement(54041..54325)
                     /locus_tag="PC460_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2599589.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54348..55865)
                     /locus_tag="PC460_000688"
     CDS             complement(54348..55865)
                     /locus_tag="PC460_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2599590.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(55990..56811)
                     /locus_tag="PC460_000689"
     CDS             complement(55990..56811)
                     /locus_tag="PC460_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2599591.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIKIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            57002..58029
                     /locus_tag="PC460_000690"
                     /pseudo
     CDS             57002..58029
                     /locus_tag="PC460_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
     gene            58101..58253
                     /locus_tag="PC460_000691"
     CDS             58101..58253
                     /locus_tag="PC460_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599592.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRTKSDLRVEFKF
                     IFKSKK"
     repeat_region   58907..60055
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=58907..58935
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            complement(60342..60566)
                     /locus_tag="PC460_000692"
     CDS             complement(60342..60566)
                     /locus_tag="PC460_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599593.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRE
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            61245..61400
                     /locus_tag="PC460_000693"
     CDS             61245..61400
                     /locus_tag="PC460_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDF2599594.1"
                     /translation="MALEVKIEVKIIKDVVILNITHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     gene            61646..61897
                     /locus_tag="PC460_000694"
     CDS             61646..61897
                     /locus_tag="PC460_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599595.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(63117..63500)
                     /locus_tag="PC460_000695"
     CDS             complement(63117..63500)
                     /locus_tag="PC460_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599596.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            63627..63956
                     /locus_tag="PC460_000696"
     CDS             63627..63956
                     /locus_tag="PC460_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599597.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVF"
     gene            64153..64362
                     /locus_tag="PC460_000697"
     CDS             64153..64362
                     /locus_tag="PC460_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599598.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFESIATLVRGGSSWSFNFIV"
     gene            complement(65353..65670)
                     /locus_tag="PC460_000698"
     CDS             complement(65353..65670)
                     /locus_tag="PC460_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599599.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
     gene            complement(65980..66336)
                     /locus_tag="PC460_000699"
                     /pseudo
     CDS             complement(65980..66336)
                     /locus_tag="PC460_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        26486 a         6956 c        11751 g        21248 t
ORIGIN      
        1 aataaaagat agagttttat ttagacattt attacaaatc aaaatttatt acatattatt
       61 tataaaaaaa tctaaggagg aaataataat gaaaatatta gtattaaact gtgggagttc
      121 ttcattaaaa tatcaattaa tagatatgaa taatgaagaa gttttatgta taggattggt
      181 tgaaagaata ggaatagaag gttctatatt aaaacatgaa aaagcaggta gagatgataa
      241 atacgttgtt gaacaaccaa tgaaggacca caaagatgca atagcattag ttttagaagc
      301 tgttgctcat ccagaatttg gtgcagttaa agaaatgaaa gaaatagatg cagtaggaca
      361 tagagttgta catgctggag aaaaatttgc aacttcagta gtaataactc cagaagtaga
      421 agaagcttta aaagaatgta tagatttagc tccacttcat aatccagcaa acataatggg
      481 aatagatgct tgtaaagcta tacttccaga tgtaccaatg gtaggtgtat ttgatactgc
      541 tttccatcaa acaatgccta aatcttcata cttatatggt ttacctcatg agctatatac
      601 taaatatggg gtaagaagat atggattcca cggaacttct cataattatg tatctcagag
      661 agcagcagag atattaggaa aagatataaa agatttaaaa atagtaactt gtcacttagg
      721 aaatggtgct tctatagctg ctgttgatgg tggaaaatgt gtagatacat ctatgggatt
      781 cactccatta gaaggattaa taatgggaac tagatgtgga gatatagacc cagctatatt
      841 accattctta atgagaaaag aaggtttaga tgctgatgga ttggataaat taatgaataa
      901 agaatctgga gtatatggaa tgactggaat ttctagtgac ttcagagata tagaagatgc
      961 agctaaaaat ggagacgaaa gagctcaagc tacattagaa gcgtatgtta aaaaagtaca
     1021 aaaatatata ggcgcttatg ctgctgaaat gaatggattg gatgttgtag tatttactgc
     1081 aggcgttggt gaaaatggaa aggctataag agcagatatt gcttctaaca tggagttttt
     1141 aggaatgaaa ttagataaag aagctaatga tgttagagga aaagaaacag taatatctac
     1201 tgctgattct aaagtaaaaa tgcttttaat accaactaat gaagaattaa tgatagctag
     1261 agatacatta agattggtaa aataattaaa ttaaacctat gcctatcaag taaaaatact
     1321 tgacaaacaa atgctagatt tgtataataa atttggtgat atgttgaggt gaacttaatg
     1381 aaagttagca tagaaaaaat aaatagaaaa gaaactgaca aaatagactt gaatttttgt
     1441 gaaaaaattg atactattag ttattgtgat gagatttaca aattagtatc acctgtaaat
     1501 ttaaaaggga aagtgtcaaa gactaataaa ggtttgtatc tagatataga tgtcaatttt
     1561 acaattgttg acaattgttc aagatgtctt aaagaagtag aaataccact agagtattct
     1621 atacaaggtt ttttagtaaa agaagaagat tatgatgaag atgagtttga ggaatttgat
     1681 ccttttatat tcgatggtga agaaatcgac cttattgata taatagaaca gacattagat
     1741 tttaatgtac ctcataaagt tctttgtagt gaaaactgta aaggtctttg tcaagtatgc
     1801 ggggcaaact taaatgaaga agagtgttct tgcagtgaaa ttacaaacga tgaggaatac
     1861 atagatcctc gttttgctaa attaaaagat ttatttaatt aaattttgac taaggaggtg
     1921 gcgtaaatgg cagtaccaaa gcgtaaaacg tctaaatcaa acacaaaaat gagaagagca
     1981 gctaactcaa agatggaagc aacaggattt gtaagttgcc cacaatgtca tgagccaaaa
     2041 ttaccacata gagtgtgtcc agactgtgga tattataaag gtaaagaagt tgtgtctaag
     2101 taatagcttc taaaaagcat gtagagtata ctctacatgt tttttttgta catataaaaa
     2161 aataagatga tttactagag aaaataagtg gattttatgc atattaaaaa atagaaaaat
     2221 aataatataa gtattgaaaa tttttgtgtg ttactatata ctactattag tagctggtac
     2281 taattacagc tataaaattt ggagggaaca tgaaaaagaa aagtaaagcc caaagacaaa
     2341 aagagcttat agatatgcta aagacagatc ctttttatac tgatgaagaa ttatcaagtc
     2401 tgtttgatgt aagtatacag accataagat tagatagaat gagtctaaac ataccagagc
     2461 taagagaaag agtcaaatca atagcagaaa ctcagagttc aaaagtaaag actttaggag
     2521 taaaggaaat tacaggagaa atcattgatt tatctgtagg gcgattaggt atatctatgt
     2581 tagaagttac acaggacatg atttactcta agactaatac tttaaaagat acgtatattt
     2641 tttctctagc agattcattg gcaatggcta taatagatgc acctaaggtt attatgagag
     2701 ttgcaaatgt aaaaagtttt aagttgatag aacaacaaga tagacttata gcaaaagcag
     2761 aagtgtatag aaatatagat aaaaagcatt atgtaaaagt tgttatcaac aataaagccc
     2821 aagagcaaat atttagagga aagtttatct ttgaagaatt agattaggag ggaaattatg
     2881 aaaatagtaa ttgatggaat gggtggagat aatgcaccta aatctaatgt agaaggcgca
     2941 gtaaatgcta taaaagaata tcaggttgat ttaataatca caggagataa agatttactt
     3001 gaaaaggaat tttcaaatta tgaatttgat agaaataaat tagagatagt tcatactact
     3061 gagattatag aaaacgaaga taaaccagta aaggctataa gaagtaaaaa agattcatct
     3121 atggtagtag cattaaatct tgtaaaagaa ggaaaggcag atgctataat atcagctgga
     3181 aatacaggag ccttacttgc tggtggatta tttgttgttg gtagaataaa aggaatcgat
     3241 agaccttgct tatgttcagc aataccaaat gtaaagcgag gaatgacact tatagcagac
     3301 tgtggcgcaa atgcagattg taaacctaag aatctagttg aatttgcagc tatgagtaat
     3361 atttattcaa gaaaagtttt aggtcttgaa aatcctaagg tagcattagc aaatgttgga
     3421 ctagaagaag gcaaaggcaa tgatttagtc aaaaggtcat atgaagaaat taaaaaatta
     3481 gatttaaatt ttatagggaa tgtagaagct agagaagtta taaatgcata cacagatata
     3541 atcatatgtg atggatttac aggtaatata ttacttaaat ctgctgaagg tgtagcactt
     3601 tcagttatga gtcttataaa ggaaactttt atggccagta caaaaagtaa aataggagca
     3661 ttacttataa aggatgattt aagaaaatta aaaagtttta tagattattc tgagtatggg
     3721 ggagctccac ttttaggtct aaatggagga gttataaaag ctcatggtag ttctgatgct
     3781 aaagctataa aaaatgctat aaatcaagga ataaaatttt caaagggaaa agtcgttgaa
     3841 gatattaacc aatttataag caaatacaat gaagaaaata aaaataacga agatgaataa
     3901 actcgttttg gaggtttaat atataatgaa tacaaaggct ggaatattag gagtaggaag
     3961 ttatctacct gagcaatcat atgataattt tcacttcgaa aaaataatgg atacatcaga
     4021 tgagtggata agtactagaa ctggtattaa ggaaagaaga tttgccaaag agtctgaagc
     4081 aacttctgac ttagcatcta aagctgcctt aaaagctata gaatgtgcaa agttaaacgt
     4141 agaggatata gagctgatta tattagctac aattactcct gatatgtctt tgccttcaac
     4201 tgcatgtata gtacaagatg caataggagc agtaaatgct actgcatttg atatatcagc
     4261 agcttgttct ggatttgtat atggagtaac tattgcaaag caatttgtgg aaacaggttg
     4321 ctataaaaat gtgcttgtaa taggagcaga aacttgttct aagtttttaa attatgatga
     4381 tagaacaaca gcagtattgt ttggagatgg agctggagca gctgttattg gccctgtaaa
     4441 tgagggtgga atactgtcta cacatatggg ctctgatggt aaaggaaaag attgtttaaa
     4501 agtacctgct ggaggttcaa gacttaaggc aagtaaagag actgtagaag ctaatctaca
     4561 tacaatagaa atggcaggta gtgatgtgtt taaatttgca gttagaaaga tggcagaaac
     4621 ttctttaaga gctcttgaaa aagcaaatct aaatacaact gatatagact acttagtgcc
     4681 acatcaagct aatataagaa tcatacaagc ttcttctaag agattagagc ttgatatgaa
     4741 aaaagtttat gtaaatatag acaaatatgg aaatatgtct gctgcatcta taccagtagc
     4801 attagatgaa gcttatagag aaggtaaaat taaaaagggt gataatgtag ttttagttgg
     4861 ctttggtgga ggtctaactt ggggggcttc agttgtcaag tggactttat agtataaaat
     4921 atctaaaatg atttaattaa caaaattaaa cttatacaca aaataaagtt gattaataat
     4981 aaaaagttta cttttatgat ttcaagtgaa ggagaattgt atgaataaaa tttgcaaaat
     5041 attaaatata aaatacccag ttatccaagg gggaatggca tgggtagcta ctgcatcatt
     5101 agcaagtgct gtatctaatg caggaggact tggcataata gcagcaggaa acgcaccaaa
     5161 agaagctata aagaaagaaa ttgttgagtg taaaaaatta acagataaac cttttggagt
     5221 aaatgtaatg cttatgtcgc catttgttga tgatataatt gatttgatta tagaagaaaa
     5281 agttcaagtt attactactg gtgctggaaa tcctgcaaag tatatggata gattaaagga
     5341 agctggaaca aaggttattc ctgtagtacc tacaatagct ttggcacaaa gaatggaaaa
     5401 gctaggagct acagcagtaa tagcagaagg tactgaaggt ggaggacata taggagaact
     5461 tactactatg gtcttagttc cacaagttgc tgatgctgta aacatacctg taatagctgc
     5521 tggaggaatt gtagatggta gaggaattgc agcatcattt gcattaggtg ccagtgcagt
     5581 tcaagtagga actagattta tttgcagtga agagtgttct gtccattcaa actataaaaa
     5641 cttagtacta aaagcaaaag atagagatgc aattgtaaca ggaagaagta ctggtcatcc
     5701 agtaagaaca ttaaaaaata aactatcaaa agaattttta aagatggaac aaaatggagc
     5761 tactcctgaa gaactggata aaaaaggtac aggagcttta agatttgcaa cagtagatgg
     5821 agacatagaa aaaggttcat ttatggcagg tcaaagtgct gctatggtaa aagaaataac
     5881 accttgtaag gaaattatag aggctatggt aaatcaagca agagagatta tgccagcaat
     5941 agaactgtaa atcaggagga gcaagatggg aaaagttgca ctagtattcc ctggtcaagg
     6001 agctcaatat gtaggtatgg ccaaggattt atatgaaaat aatactgttg ctaaaagtgt
     6061 aatagatgag gctagtgatg ccttaaatat ggatttaaaa aaccttatgt ttaatggaaa
     6121 tgaagaagaa ctttctaaaa ctgaaaacac tcaacctgct atggttacac atagtgtagc
     6181 tgtattaaaa gcagtacaag cccaagtaga tttaaaatat agtgcatgtt taggtttatc
     6241 tttaggagag tatagtgcat tggttgcagc agatgcaatt gactttaaag atgctgtatg
     6301 cttggtcaaa aaaagaggta agtttatgca ggatacagtt ccaacaggtg ttggagctat
     6361 ggcagcaatt ttaggtctag atagaagtat attagaaaat gtagttaaag atatacaagg
     6421 aggtattgtg gaagtagcaa attataattc tcctggtcaa atagttatat ctggtgaaaa
     6481 tgaaaagata gaagaagcaa tggtgaaatg caaagaagca ggagcaaaaa gagctgttaa
     6541 attaaatgta agtggacctt ttcactcatc aatgctaaga gaagctggtg taaaattggc
     6601 cagtgaatta gaaaaagtta acataactaa acctaaagtt gatgtggtgg caaatgtaaa
     6661 tgcagattac tataaaaacg aaggtaaaga cctgcttata aaacaagtaa gttcatctgt
     6721 tctttgggaa gattctatag aaagactttt aaatgatgga tatgatactt ttatagaaat
     6781 gggacctgga aaaactttaa aagcatttat taagaaaata gcttccaata aaaaagcaaa
     6841 tgtcaatatt tataatatag atggtataga ttctcttaat gaatttgtac aaaattatag
     6901 aaatggagag atataatgat aaatcttaca ggacaagtag cagttgttac tggtggctct
     6961 aggggtatag gaaaagaaat agcaaaaaaa ctagcatctt ttggagctga tgtagtaatc
     7021 aattatactt ctaaagaaga tgaagcacta aaaactaaaa atgaaataga aagtatgggg
     7081 gtaaagtgta cctctataaa atgtgatgtg tctaaatttg atgaagtgaa tcaaatgata
     7141 gattctgttg taagcgaatt tggaaaaatt gatatattgg ttaataatgc aggcataact
     7201 aaagatggtc tgcttatgag aatgaaagaa gaagattttg atagagttat agatataaac
     7261 ttaaaaggtg tctttaattg tacaaaagca gttactaaac ctatgatgaa aaagaagtat
     7321 ggaagaataa taaatatgac ttcagtagtt ggaattatgg gtaatgcagg gcaaactaat
     7381 tattgtgcat caaaagcagg tgtaattgga tttacaaaag cttctgcaag agagttagca
     7441 tcaagaaaca taaatataaa tgcagtagca cctggattta tagaaacaga tatgactaaa
     7501 gtactaagtg atgatgtaaa agaatcaaca ctagcaaaca taccaaagaa atcttatggt
     7561 aaaccagaag atgtagccaa tgccgtagca tttttagtta gtgacatgtc aagttatata
     7621 acaggacaag taataaatgt agatggtgga atggtaatgc aataataatt taaaaagtag
     7681 agaaatctat taaaataaaa aattttagga ggtacacaat gtttaataaa gttgtagaaa
     7741 taataataga gcaattagga gtagaagata aagaaataac tatggaaaca tcattaatga
     7801 aggatttaga agcagattca ttagatgcag tagaaataat aatggcactt gaagatgaat
     7861 ttggtataga aataccagat acagaagcag aaaacttcaa atctatagga gatatagtta
     7921 agtatataga agctaataaa tagtcgagct ttaaaaaagt ggaggtatcc gatgaataaa
     7981 agagtagtaa taacaggact tggatgtgtt acgccgttgg gaacaggaaa agaagagttc
     8041 tggagtaata taaaatctgg agtatctggt atagataaaa taactaattt tgatgctagc
     8101 acatatcaaa ctcagatagc aggagaagtt aagaattttc atccagaaga atatataagt
     8161 aaaaaagaat tgaagagatt agataaattt gctcaatttg caatagtatc tgctaaatta
     8221 gcagttgaag atgcaaatct tgatttagat aaagttgata gagaacgttt tggtgttata
     8281 ataggttctg gaataggtgg agttgaagct atagaaactc aacataaaat tcttttggaa
     8341 aaaggaaata aaagagtaag ttcacttttt gtacctatga tgataggaaa tatggcagct
     8401 ggtcaagttt caatattttt aggagcaaaa gggcctaaca ctaatgtatg tacagcttgt
     8461 gcatcaggaa ctcattcaat aggtgatgct tttaaggtaa ttcaaagagg tgatgcagac
     8521 atcatggtag cagggggttc tgaagctgct gtaacaggtc ttgcttttgc tggattctgt
     8581 aacatgaagg ctatgtcaac tagaaatgat gaccctaaaa ctgcatcaag accatttgat
     8641 aaagacagag atggttttgt aatgggagaa ggagcaggta ttgttatctt agaagactta
     8701 gaacatgctt tagcaagagg cgctaaaata tatgcagaag tagttggata tggtttaact
     8761 gctgatgcat accatatgac aactccagct gaaaatggag agggagcagc taggtctatg
     8821 aatatggctt tgaaagatgg aaatgttcca ttagaagaag tagattatat aaatgctcat
     8881 ggaacttcaa cttattacaa tgatttatat gaaactatgg ctataaaaac tgtatttgga
     8941 gaaaaggcat atgacttatg tgtgtcatca acaaaatcta tgactggtca cttattagga
     9001 gcatcaggtg ctatagaggc tgttgtttgt gctatgagta tagaagatag ttttgtgcct
     9061 ccaactataa acatacaaga agttggtgaa gatttggatt tagattatgt tccaaatcaa
     9121 ggtaaagaaa aaaatattag atatgcacta tcaaattctt taggatttgg tgggcataat
     9181 gctacaatag tactaaaaaa atatgtataa ataaatatta aatataaaac taaaaaacaa
     9241 gcacttgttt taactgtata aatttatatt agcttaaatt taaagtctcc taaattattt
     9301 taggagactt ttttattaag atttaagtct agcatatgtt gtaaatcatt ttgatatatt
     9361 gtattattaa ttattaagag gttatttata tatgtaagtt ttcattctac aatatacaaa
     9421 aggaggacta aaattttgaa ttttagacta ccaaagaaaa ggttacccaa aaagataatt
     9481 tttatatgta taatacttat tagtattatt ttgtttataa agcatgacca aaatggattg
     9541 aaagaaagag aaattataaa taagggaaaa tatatgtctg aaagccttgt ggcagataag
     9601 gagtatgatg aagcaaaaaa agtggttaag acttctttcg aatctattcc agaaaaaaaa
     9661 tacaataaag tatataatga agtttgggat atgttaaaga ctatttcata tgttccagat
     9721 ggaacgaaga tagtcattga ttctttagaa aagatgagag aatcagatgt gaaattatct
     9781 gatgaatgta gatttaatat agaaaaaaga ttagcatcag tgtatataat ggataataat
     9841 tattctaagg cagtagattt aactgtaaag tctataaagt tggctgagaa attggggaat
     9901 aattatgaaa aagcaagact agatgttgat ttggcgagca tatttttaaa gataggtggc
     9961 tatgaaacag gagagaaatt aataaaggat tcttttaaaa tagatattga tgaaggtgaa
    10021 aaaaatggaa tggttagatt atatgctatt ataaatctag cagaaatata tgtagaagag
    10081 ggcgaatatg ataaagctat tgaaataagt agcaggataa aagattataa aaagtttgta
    10141 aatacggata attacaatga ttttgatata atggcctcta taatgcagtc aaatgcgtat
    10201 gtgggcaaaa ataatataca aaaggcaaag acttttttag aaaaagctga ttatcttata
    10261 aaatcagata gaacagttta tatattagat aaagatatgt actattatat ggcaatggga
    10321 aacttagaat ataaaagcga aaattataat tctgcaatag ataattataa aaaaagttta
    10381 gagatatcta ctaaaagaaa gttaacccag gagaaaataa aaaatttaaa taagatatta
    10441 aatatatatg aaaaacaagg aaatatagaa tctttaaatg agtatcaaaa gatgttaata
    10501 aatgaatata aagagaacaa aagtataaga gattcagaga ctagtttcta tataattgat
    10561 aaagtatcaa acgaaagtaa gctatttgaa aaaactaaaa aagaaataaa atattataaa
    10621 atattattta cagtaatttt aattgctata ttagggtcta cttttttata caaccgattg
    10681 aagtatttta aagcacaaaa tttacatgat ggtcttacaa atgtttataa tagaaaaagt
    10741 tttgatatta tgtatgaaaa gtacagagag aaagatgata attttgcact tgtgatgata
    10801 gatattgata atttcaagct cataaatgat aattatggac ataaatttgg tgacactgtt
    10861 ataaagggaa taacaaatac catttgtgtt atgttagaac aagatgataa agtctttagg
    10921 tacggaggag aagaattttc tgtattaata agaaataaat ctggtgatga agtagcagat
    10981 ataattgaca gtataagggt tgcaattgca aataaaaagt ggaatgatga tgtgactgtg
    11041 actataagtg caggtgtagc tcatgtttca gatagcaaag atgtactaga agaagctgat
    11101 aaaaatttat ataaagcaaa acaacttggt agaaataaag tagtatataa gtaattatat
    11161 actatataaa taagaggtat aaatatgaaa caatcaatag ctatagtaac tgataaaata
    11221 actaaactat catctttctt agaagaaaat atacgtttgg ttttaggtaa ttatattgat
    11281 ataaaccact attatctgga agttttaaaa gagaatgata aaatacaagg tgattttgta
    11341 ctagtaatga atgatgatag attaaacagt atgagaaagc atttagtaca agatagtaag
    11401 attatagtga taaggagaac acttaaagaa aatgaaatat atggattatt ttcaatacca
    11461 aatggaactg aagtattggt tgtaaatgat acaaaagaaa ctaccttaga aacgataagc
    11521 ttattttaca aaataggagt aaaaaattta aagtttactc catatattga aggaaaaatt
    11581 tataaaaata tagacatagc tataacgcct ggagtaaagg gaaaagtacc aagttatata
    11641 aataaagtaa tcgaccttgg aaatagatat attgatatat ctacctttat agaaataata
    11701 aatggtctta gaatagatgt taaagaaata cgtaaaaatt taatggcata ttctgagagt
    11761 ttaataagtt tagataatgg agttaaagat aattataggc aactgttttt aaaaatagaa
    11821 gaacaagatg ttatattgaa tctatcaaaa gatggaataa tatttacttc agcagatggt
    11881 ataataaaca catttaataa agaagcttta aaaattttag gtataaatga aaatccacaa
    11941 ggcaaaaaca tagaagatat catttctgaa gatttaaata ttttacttga agacaaagaa
    12001 ataattgatg aagtggtatt tacaaataaa aaatatataa atgtaaataa aaaaagtatt
    12061 ttttctatgg ggaataaagc tgggatatat tataacttac aagaaataac gtatataaaa
    12121 aaattagaac aaaatcttac taataaatta agagaacaag gtcagatagc aagatataca
    12181 tttaaagata taaaaactaa aaataaaaat atggtaaagt gtgtagaatt ggctaaaaaa
    12241 atatctaaat cagaactttc tgtattaata ataggagaaa gtggtactgg taaagaatta
    12301 ttatcacaat caattcataa tgcttccaat agaagtaatc aaccgtttat agcagttaat
    12361 tgtgcagcag ttccagatag tctattagaa agtcaactgt ttggatatga aaaaggctct
    12421 tttacaggag ctttaagaga aggaaaaaag ggtctatttg aacttgctaa taatggaact
    12481 atatttctgg atgaaattgg agatatgcct ccattgctac agacaaagtt actaagggta
    12541 ttacaagaaa agcaggttat gcctataggt tcccataatg ttataaacat agatgttagg
    12601 gtcatagcag caacaaacaa aaacttacta aagatgatta gagaaggaaa atttagagag
    12661 gacctgtatt atagactaaa tgtattgcca atagatgtac catcattgag gaatagaaaa
    12721 gaagatataa taacacttat gaatttcttt ttgaaaaaca atataagact ttcgccagaa
    12781 gttataacta ttttagaaaa atataattgg ccaggaaata taagagagtt acaaaatgtt
    12841 gcttcttatg taagtctaat gtgtgatagt atggttttta gagatgactt accaccatat
    12901 ataagggtag aatgtgctaa caattatgaa gatggatttg gttatacaag aaaagaattt
    12961 gaggaaatac tgaggattct atatgaaaga agagagtcat cctctggaat tggaagaggg
    13021 tttataatcg aaaagcttat acaggaaggt attgagattt ctgaaagcaa agttagaaag
    13081 gtgctatctt atttaagtga atgtggatac atatcatcta aatctggtag aggaggaagt
    13141 caaataacct cagaggggat aaaactttat aataatataa attaagctaa gtatttgata
    13201 ttgaaatcac atattttgaa tttagatatt taaataataa taaactttta tatttataga
    13261 gattaattta tattatgtca gaaagaatat tgtaatttat aattaaatat taataggttt
    13321 ttaaaatagg attagatagg taataggtta agttagaata acccattacc tatttttatt
    13381 tgtaagaaaa gtgtacatag aattagaagt aaaaaataca actaataaaa ctattgaaaa
    13441 tactagataa ataataaata aaaataatta tagattttgg catacatatt gctaattata
    13501 gagataagac gacaaaataa ttgggaggtt taatgatgaa atacaaaaca acagggactt
    13561 gtgctacaga aattgaattt gaagtaaaag aaaataaagt tacaaatgta aactttatag
    13621 gtggatgtga tggaaattta aaagggctaa aagtattagt tgaaggcatg aatatagaag
    13681 atgtaataca aaagttaaaa ggaatagagt gtaaaacaaa gccaacatct tgcccagacc
    13741 aactgtctct agcattggaa aattatatga atataaagta atacataaaa caatcgatta
    13801 gttaggagtt taattatgaa accaataata gggattttag gaaatttaat aataatggaa
    13861 aatggtatgt ttccaggtct tgaaaggtca tatgtaaata atgattatat aaatgcagtg
    13921 ctaaaaggag gaggtagtcc agtaataatt cctgtaaata cagataaaga agttataaaa
    13981 aaacagattg aaatggttga tggagtctta atatctgggg gatgggatat caatcctcaa
    14041 ctgtatggag aagaaacaag agaagaaacg acatttatat atccagaagt ggatgaattt
    14101 gatttaattg caataagtat agcattagaa cttaaaaagc ctatattagg tgtatgtagg
    14161 ggactacaaa ttttaaatgt aagtttagga ggaactttat atcaagataa taatcttata
    14221 gaaggaagtt atataaaaca tacacaatct tctaaaagac atgtagctac tcataaagtt
    14281 gatgttaaag aaggaagtat tttagaggga attttaggaa aacaattact tacaaatagt
    14341 tatcatcatc aaagtgtcaa tcagcttgga aaaggattaa aagccatagc atactcaaaa
    14401 gatggtataa tagaagctat tgagaaagag gatgaaaatt ttgtagtagg agtacagtgg
    14461 catccagaaa tgatggtaga ttactgtgat aaaatggaaa aactatttaa atattttata
    14521 aatatttgtt caaatttata atataccttg gggggattga acatgaacga aaagaacaaa
    14581 atgggattaa taagtattat acttttaggt attaatgcag tagtaggagc tggagttttt
    14641 ctattaccag gagatgcaat gaaatcattt ggtgtagcaa gtatatttgt atatatattt
    14701 gatatgttgt tagttttatc tatggcattc tgttttgctg aggttgcagg taaattcaat
    14761 aaaaatggtg ctgcctatgt atatacaaaa gaagcttttg gagatttttg tggttttgaa
    14821 gtaggtctta tgaaatgggt aataggatgt atttcttggg gagctttaat agtaggtttt
    14881 cctacatcat tatcagcagt atgggctcca gcaggtgaac cacatataca aaaaattata
    14941 attgtagtca tgatagttgg gcttacaatt ataaacttat taggtgtatc tctatcaaaa
    15001 attgtacaaa atgttataac tgtaggtaaa ctaattccat taatactatt tataggaata
    15061 ggtatatttt ttataaaagg agttaatttt acttcttcaa caatggtacc tccaggagct
    15121 ggagcaacag agtttggagc agcagcactt ttaatgtttt attcatttac aggatttgaa
    15181 tctatagcag ttgcagcaga agatatggag aatccacaaa aaaatatacc aatagcaatt
    15241 ataagtgtaa tagtaattgc atcaataata tatatattaa atcaagttgt atgtgttggt
    15301 atactaggag attcgttatc tagtacatct acaccagtgg cagatgctgc aagaatatgc
    15361 tttggaaata tgggagctgg attggttacc tttggaacct tggtttctgt tggtggaatt
    15421 tgtatgtgtg gtgcttttgt aaatccaaga agctgtgtag ctttagctga tgataaaatg
    15481 ttaccgagaa tatttgcaag aaaagataaa aaaggtacac catatgtagc gataatagct
    15541 actatgctaa tcactatacc aatagctcta tctggaagtt ttgctgaatt agcagcaata
    15601 agtgcagttg ctagattcat acaatacata ccaacatctt tatccgtatt agtgtttaga
    15661 aagaaaagac cagaattagt aggtactttt aaaactcctt ttggagcagt gattcctcta
    15721 attgctgtat gtgttggaac atggttatta ttccaagcat ctatgcatca attaattatg
    15781 ggattaggag ctttggcaat tggagtacca ttatacttta taatgaagtc ttataataga
    15841 aaagtctatg atgaaaattt aaagaaaata gtttaattat gttataacaa tttattgagt
    15901 taaaattaga cattggcata aaattaaata caaataagta tctacaaggc tatattaaaa
    15961 tattattaat tttttatttt aatatagcct tatattgtta tcaataaatg aaaaatattg
    16021 taacaataga agaacaatag tattatgttt atagacaaaa aacttattag gaggtaaatt
    16081 tatgccaaca ataacattaa aaaatggtgt agacgtactt attagagaag gtgtaagaga
    16141 agatgctcaa agcattatag atttttataa tgaagtgggt ggagaaactc attttctgtc
    16201 atttggaaag gatgaatata aaatatcttt ggaggaacaa gaaaatgcta tagaatcagc
    16261 aaaagcatcc gataattctg ttaaactgat tgcatttata gatggtgaaa tagttgggat
    16321 tgcaacgatt gattctaatc aaaaagcaaa aggaaagcat gttggagtat taggaattgt
    16381 tgtaaaagaa aaatattggg gaattggatt gggtaaaagg cttatgttag accttataga
    16441 atggtgtaaa tcaaatggaa taaccaagaa gataactctt gtcactaatg aagaaaatta
    16501 taatgctata ggattataca aaaaagttgg atttgaagta gaaagcatac taaagaaaga
    16561 atgttattat aatggtgttt atactgattt aataggaatg tcacttttat tgggaatata
    16621 attagtgtac attttatctc gaaaaaagta tattaagtat ggtataatta aaaaagaata
    16681 taacttaata taattttgga ggacgaatta tgaaaaatct gtgtaaaata tcagatgatt
    16741 atttaaacag caagcttaag tatttaaagt tattatcaaa acaatatcca agtatatcaa
    16801 aagcaagtac agaaataata aatttagagg ctatattaaa tcttccaaag ggaacagagc
    16861 attttataac agatgtacat ggtgaatatg aaccatttgt acatgtatta aaaaatggtt
    16921 ctggtgtaat aaagagaaaa atagaagaat tgttttcaaa tacaataaga gacagcgaaa
    16981 aaaaaatgtt agcaactctt gtatattatc ctgaacaaaa gttagaccta ataattaagc
    17041 aagaggaaaa tatagatgat ttttatagaa taaatattta tagacttata gaactttgta
    17101 aatatgcttc tagcaaatat actaggtcta aagttagaaa actgttacca gaaaatttta
    17161 aatacataat agaagaactt ttgcatgagc atgttaaaag tgaacataaa gaagagtatt
    17221 ataaaagtat agttgaaaca atagttgaca taggaatagc taaggagttt ataattgcta
    17281 tttctacagt tatacaaaaa ttggttgtag atagacttca tgtaatagga gatatttatg
    17341 atagaggtcc aagaccagat ataattgtcg ataagcttat tgaacatcat tgtgtagata
    17401 ttcaatgggg aaatcatgat atattgtgga tgggtgcagc ctcaggtgaa aaaacttgta
    17461 tagcaaatgc actgaggata tcagctagat atgcgaattt agacattgta gaagacatat
    17521 atggaataaa tctattgcct ttagccactt ttgctataga aatgtataaa gatgaccctt
    17581 gtaaagaatt tattccaaag gttaatgacc aaagtgtaac aacaacagaa aagtctttaa
    17641 tggcaaaaat gcacaaagca ataagtataa ttcaatttaa gcttgaaggt gaggttatca
    17701 gacgaagacc tgaatttgaa atggaacata gactcttgct taatatgata aactatgatg
    17761 aaggtacgat taatttaaaa ggtaaaacat ataaattaaa agatacttat ttacctacaa
    17821 tagacaaaaa agacccatat aaattaacta tggaagaaag aaatgttatt gataaattag
    17881 tatcttcatt tagaggtagt gaaaaattac aaaaacatgt ttcatttttg ttttctaaag
    17941 gaagtatata tttaaaggct aactcaaatt tactgattca tggatgtgta ccgttaaatg
    18001 aagatggaag ctttatgtct atgaatataa tgggaaaaga gtataaaggt aaggctctta
    18061 tggataagat ggagtctcta gcaagagaag gatttttctt taaagataag gcagaagaaa
    18121 aactgtatgg catggatatt atgtggtatt tatggactgg gaaatgttca tcattatttg
    18181 gtaaagatga tatgactaca tttgaaagat actttatagc tgaaaaagag actcataaag
    18241 aaaataagaa cccatatttt aaacttagag aaaatgagat ggcttgtaaa agattatttg
    18301 aagaatttga tttagaactt gatgaatctc atataataaa tggtcatgtt cctgtggaga
    18361 gtaaaaatgg agagagccca ataaaagcta atggtaaaat tctcgttata gatggtggtt
    18421 tttctagggc ttaccaaaaa actactggta tagctggata cactttgata tacaattcta
    18481 gaactttgca attagtatcg catgaaccat ttaattctgc tgaagaagcc attgcaaatg
    18541 aaagtgatat cttatcgaca actgttgtag tagagcataa ggcaaagaga aaaatggtga
    18601 gagatactga tgaaggagta aagatacaag aggaaataga agatttaaaa ctacttttaa
    18661 tggcttataa gaaagggcta ataaaagaaa tgtagatgtt atagaggcac ttcatgattg
    18721 aagtgtcttt ttgttaatac ttattaaata tattataaag attaataata gtagtttata
    18781 ttatacttac taagtgaatc caagataaat gggttcactt tttttattta taaacattat
    18841 taaaaaaata tagatgtttt ggttatattc cttgtccttt ataaatagaa ttatataaac
    18901 aaaggaggag gacaagagat gaataaactt tctgatgaaa taataaactc tctttctaca
    18961 actataagag aaaaggtaga aaaagtctca aacaataatc tgaacataga agaaattagg
    19021 ttacgctcac aaaaaccatt gatactaaat gcaaactcta aagattattt ttacaatcaa
    19081 aaaactatga cacttgattt aaatcaacaa aactcatatg tagtaacaag agaagatgta
    19141 gagcaaactt ttcaaataat atgtaaatat tcaatacatt catttatgga tgacataaaa
    19201 aaaggattca taactttgag aggaggtcat agagtagggt tagttggaaa agcaatagta
    19261 gaagatggac aagtcaaaaa tataaagcat atatcatctt taaatataag ggtatcaagg
    19321 gaaattattg gatgctcgga taagatttta agtcatatca taaagggaaa aaatcagatt
    19381 aataatactt tgataatatc accacctcaa tgtggaaaaa caactttaat aagagatata
    19441 gtaagaaatt taagtaatgg taatgaagat tatggattta aaggtttaaa agtggcttta
    19501 gtagatgagc gtaatgagat agcaggagct tatctaggtg ttccacaaat ggatgtggga
    19561 ataaggactg atataataga aacttgccca aaagacttgg gtattacaat gcttttaagg
    19621 tctatgtcac caaatgttat tgtgacagat gaaattggaa gtgaaaaaga aataaaggca
    19681 ttatacacag cattaaatgg aggtatagga ttaataacta ctgtgcatgg agattcaata
    19741 gaggatatac aaaatagaaa agaattaaat agacttctgg ataaagagtt atttaaaaag
    19801 gttataattc tttctgcaaa aagaggagca ggaacaattg aaaaaattta tgatttagaa
    19861 gaaaagagat ggtattttgc aaattaaaat aattattata gctttcttga taggaagtag
    19921 ttatttgata ggtgaacaaa tatacaaaac ttatactcgt agacataaac aattaaacga
    19981 tttaataaga gttttagaaa ttttgaggat ggatttatct tttggacttt atacattaga
    20041 agaaatcttt aataggatag gtggaaataa agagttttgt ttttggaaat ttttctatca
    20101 aatatcagaa ggacttcaca atgaacaaag taaaactttg gaaataataa tatcagaaaa
    20161 tattgatgtc ttaagtaaag aaacttatct aggcaataaa gaaattgagg aactgaaaaa
    20221 tctaatactt actttaggga aaagcgatat agagtcacaa caaagaatga tagatttatc
    20281 tatagaaaat ttgaaaaaac aaacctatga gacaaaggaa gatataaaca aaaaaggcgt
    20341 attgtataaa aaattagtga cattcatagg tataggtata tgtattatat tgatttaata
    20401 tggggaggtt tattatggaa atatcattga tattaaaggt tgcaggtgtc ggtattctaa
    20461 tatcagtatt gaatatgata ttggaaaaaa cagatagaaa agattgggca ggtcttacta
    20521 cattagcagg tgtaataatc gtattgggta tggtaataac agaaataagt gacttgttta
    20581 atactgtaag aacaatgttt caactttact aaagaggagg gagtaatttg gaaataatgc
    20641 aattaatagg aattgcaatc atttcaacta cactctgttt agtaattaaa aaagatagac
    20701 cagagatagc aaatttcata gctataataa ctggagtaat aatactattg tcagttatgt
    20761 ttaagctaaa ttttatagta gatagtatac aagatttggc taacaaggct aacatcccga
    20821 ctatgtatat atcattaata ataaaattaa ttgggatagc atacttaatg gaatttgcga
    20881 tacaactttg taaagattgt ggggaaggaa acattgcttc aaaactagaa tttggaggta
    20941 aaataatagt tatgtcaatg tctttcccta tacttttgtc cattgtagaa atggtggtga
    21001 atataattcc atagatgtgt atcttggaat gaatataagt atattagaga gcttttgttg
    21061 tattgaggtt tatagagtga agagaggcat ggttgcttat gaaaaaaaga tttttaccaa
    21121 tgattatagg ttttttattt actttctttt ttgtaaatat ctttgctatt atgatatttg
    21181 ctaatgaagt accaagtagt gaagataaag aatatggtga aacaaaaaat agcatagata
    21241 agtatataga tggtcaatta gacaagctag atataaatga gattcaagac tatataaata
    21301 aagaaatagt tattaatgat gttaatttaa aatcatttgt aaaagattta attagtggag
    21361 aaaaaaatat cttagattta ttcaataaag atggattgaa gatattgatg tttgatgaat
    21421 ttaaagcaag tttaaaggta gtagcagtaa ttttggtact ggctctgtta tcatctattt
    21481 taaagagtct ggaaaactct ttttcatcag gtgcagtaag tcaaatagca acatatataa
    21541 tatttatcac aatggtatca cttacactta ttggttttaa agatgtatta cagatttgct
    21601 atgatgcaat agaccatact gtaggtctta tgcaagttat aatgccaata ttaataacat
    21661 ttttactttt aattggattc ccaataacgt cgactacttt gaatcctatc ttcataggag
    21721 gagtaacatt tataaatgta ttttttaaaa attttctatt tgtatctatt actgttgcat
    21781 ttggaatttt aataataaac aatttatcaa aaaatataag gctaaagaga tttttctcct
    21841 ttgtaaaaca aataaattat gtttctattg gagctatgtt tactgtttac ttaggtcttg
    21901 tatccataca aggattgtat gtaaccagtt ttgataaatt cagtgtaaag actgctaaat
    21961 ttgctatagg taattttatt cctgttgtag gtggatttgt atctgactct gtggatattt
    22021 tattatcttc atctcagctt ataaaaaata tatttggagg aatagggctt atcttacttg
    22081 ttggaatttg cttacttcca gtaataaaaa ttttatcagt aatagtagtg tacaaattag
    22141 cagcaataat tgtagagcct gttggagagg atggaatatc taatttttta aatgaagtgg
    22201 caaatttaat gataataatg ctagcatcag taattgctat aacagtaatg ttctttgtaa
    22261 ctgtagctat cttgacctcg ataagtgtcg taagtcaagg gtaatttata tatcagtgaa
    22321 aaataattac atttaatcta atgtttttag agaggaggat gctatgttag agggcataaa
    22381 ggcatggata gttagtgttt tgataggagc atttatagta aatatagtag atatgatttt
    22441 accaagttct aagataaaac cttatgtcaa cctagtttta aactttatgt ttgtatttat
    22501 tgtaataact ccagtagtag gatttttttc aaaagatatg agtttagaag atagaatatt
    22561 aaaatctatg ggaaactaca acaagcagta tgtagatagt acaaatgctt tggcaaaaga
    22621 gactggaaat aatagtctat ccaagggata tgaggatggg ttaaaagaag tacttaaatt
    22681 aaaacttgat gaatatggtt atgatttaga agatatagag cttaatgggg caaatataaa
    22741 taatataaaa ataaaagaaa agaataatag tgcaaaaagt aataacagtt ccaatgaaga
    22801 aaataaaaat aatagtacta aaagagagga agaaaacata aactctaatg acaaagaaaa
    22861 ttctaaacaa gtcttcaaaa aaggtacaga gtatgggttg aacctaaatg aagaaaaatt
    22921 aaaaaacgac ttaataaagg tgctggatgt ttctatagaa gatatacaaa ttgataaata
    22981 ggagttggga gaatgttgaa aaacttaaat gacaaagata agagaaaggt atattcacta
    23041 ataactatag caggaatatg tgtggtttca ttagtattat tatcttgttt tcctagtagc
    23101 aaaagcgata aacaagttgg aaaaacagag actaataaaa atgctgaaaa gcaagtcact
    23161 aaagaacaag aaaaagatga tttagagtca aaattaacag caatactatc taaaattgat
    23221 ggtgcaggag acgttgatgt aatggttaca tttgaatcaa gtgaagaaat acaacctgct
    23281 tttaattcaa ataatacaac agaaacaaca gaggaaaaag atgcacaagg tggagaaaga
    23341 acagttacga cttctagtga aaataaaaca atgataactt caaattcaag tgaccctgta
    23401 gttataaaaa ctacagagcc aaagataaag ggagtgatag tggtagcaag tggagcaagt
    23461 gacccaagtg ttaaagagac actttatagt gcagtacaaa catcattaca agtagctggt
    23521 catcaagtag aaatatattc taaataaata catataaccg gggggataaa tgaatgaagt
    23581 ttaattataa gggaagagga tttgtaataa taactttaac tgcaatgtta gtggtagtag
    23641 gaacagtgaa ttatcaacta agtaagaaat ctttattaga aacatcaaaa gagtttaagg
    23701 catatgaaga agcacagttg caaaaaaata ctgatgatag ttatagtagt aagacagagg
    23761 attctagtaa tgtagataaa caaggtggta aggaaagtgc tgatattgat atagtcgata
    23821 gcaaagctag taaagtaaaa gaaaaggcaa ctgagacaag taaggaaata aaagctcaat
    23881 tatcatcaga gaaaaatatg aaaaaggcat cttatatttt agatatgaaa atgaatagag
    23941 aaaaacaaag aaatgagtta gttcaagatt taaatgaaat gataaataat ccatctacaa
    24001 cagaagaatc tagaaaagaa gcttctaata tgaaattaaa tatagttaaa attcaagaaa
    24061 aagaacttca aatagaaaac cttttgagta caaaaggata tgaagaagcg ttagtttata
    24121 taagcgataa caaagttaat gtagttgtaa atgaagctaa attagaaaag aaagatgcag
    24181 caaaaatatt tgatttagtt gcagaacaag ccaatgtgaa gtatgaaaat attaaactta
    24241 caaataataa tagtaataag taaagtataa acctcagaat taactgaggt tattttcttg
    24301 caaagtagac ttgtatttgg tattattata cttataaaaa tagttcctta aaatcaggag
    24361 gtaagtttta atatggaaga taataaattt ggacaagtaa aaatatctaa tgatgtaata
    24421 gctacaatag ctggtttagc agcattagaa gttgaaggca tagaaactac agcaacatta
    24481 acagataagt tgttaaaaaa taatggagta aaaatacaaa tcgaagaaga ggatgttaat
    24541 ttagatgtaa tggttacgat aaagtatgga atgtcaatac cagatacagc ttttaaagtt
    24601 caagaaaatg taaaaaatac tgttgagaca atgacaggac tgaaggtttc tcaagtaaat
    24661 atacacattc aaggaatcag ctttaagaag gataaagttg ataaagaaga agcaaaagca
    24721 actaagaaaa attaatatta gccctctgaa aagtcagagg gttttttgct ggtaattgat
    24781 tttatatgga taattaaact taaaggagaa ttttgattga tgaaaaaaga tagggcacaa
    24841 aagagtacaa caagagaata tataatgaaa cttatatacc aaataaatat taataaagaa
    24901 gattttgaaa ctttagaaga taaagtagat aattttttaa aagataattc agaacatatt
    24961 ataaatagat ataaagaact tgcacttcaa tactcaaaaa atacaaattt aaagttagaa
    25021 gatactgaaa ttgaagatgt tatagacaaa aaatatataa atacagtatg caaagcattg
    25081 aaagaaaatc atgacaaaat agatgagtta ataaataagc atgctaaaaa ctggacagta
    25141 gatagaatgc caaaggtaga tgtttctata cttagattgt ctgtgtgtga aatactttat
    25201 ttagacactc ctaataaagt atcaataaat gaagcagtag aacttgcaaa aatatattgt
    25261 gatgataagt ctcctaaatt tataaatggt atattgggaa gtgttgttga tgaaattggg
    25321 aaataatata ataattggaa ttgatactag ctgctatact acttctatag cagctatctc
    25381 tttagataag aaagttatat tcaatgaaaa aataatgtta gaagtaaggg ataattcaaa
    25441 aggtttaagg caaagtgaag ctgtatttca acatatcaat aatttaggga ttcttagtga
    25501 taggataaaa tcttttaaag ataagtttaa tgtagaagga gtttgctcat caaaaaaacc
    25561 gagacctgtt gaaaactcat atatgcctgt attcaatgta ggtcataatt ttggaaaatt
    25621 attgtctagt atatatggtt gtagatttta tgagactact catcaagaga atcatattga
    25681 agcaagttta ttaaatagta aattaaaaaa taataataaa tttatttctg ttcatatgtc
    25741 tggtggtact acagagatac ttttaacaag caaacaagat agtcatcaca atgtatgtga
    25801 tactaattta ggtaaaattg ccaaaataag tattaaaaaa gatgataaaa gtaagttata
    25861 caataatttt ggatataata tagatataat tggaggaagt aaagatatta gctttggtca
    25921 acttatagat agagtaggaa ttaagcttgg atataaattt ccttcaggaa agtatttgga
    25981 tgaaaatgct ttaaattgta atcttaaaat agaaagtggt ctgaaaactt ctgtaagaga
    26041 tgggtatatg aatttatcag gattagaaaa tcaggtaaat aagattataa atgataatgg
    26101 agataatact aatcaaaaag aatatatatc taaattagta ttagattcag tagttagaaa
    26161 tatgtttaaa tctctagtgt atttatgtga aacttataat gtaaatgaag tagtatttgc
    26221 tggtggagtt tctgcaagta agtatattct tagagaatta agcatgaagt taagaaaaaa
    26281 gcatatagaa gcttatttta cagaaccaca atattcaact gataatgctg tcgggtgtgc
    26341 tataatagga ttgaataact ttttaggaga aagagtatga aactaagagc tttagatata
    26401 agtgaagcaa actcatatat taaaagaatt ttaataaacg acccaatact ttctaatctt
    26461 aaagttaaag gagaaatttc taattttaag gttcatagta gtgggaatgt atatttatca
    26521 cttaaagatg aaacatcaaa gttaaactgt gtaattttta aaagtaattt taatcgcaat
    26581 ttaaaattag ataatggtgt aaaaattata gctaatggat atatatcagt ctatgaaaga
    26641 gatggtgcgt atcaattata cattaatgaa attgaaatag agggaatagg aaatttgcac
    26701 atagagttta atagattaaa ggaaaaactt aataaagaag gattatttga ccctaaatac
    26761 aagataccta tacctaaaat gccaaactca attggcgtta taacatctcc aacaggtgct
    26821 gttataagag atataataaa tgtaataaaa agaagatatc cgaaagttaa tataaagctt
    26881 tatccagtaa cggttcaagg agataaatca gctgaagaaa tctgtgaagc aattagattt
    26941 ttcaatcata tgaagaatgt tgatacactt atagttggta gaggtggggg ttcaatagaa
    27001 gaactttggt cttttaatga tgaaatggta gcaagagagg tttttaattc tcaaattccg
    27061 ataatatcag cagttgggca tgaaacggat tttactattt gtgactttgt gtctgatatg
    27121 agagccccaa cgccatcagc agcagctgag attgctactc catcattgga tgatataaac
    27181 tataaacttg gaaacataaa aagtaggatg agtaagtctc ttacaaatca aatagagcta
    27241 gaccaatata ggttagaaac agtatttaat aagataaata actatttaga ttcgtacaca
    27301 ataaaagata aagttataca attagacaaa atatatgata aaataatttt tggaatagaa
    27361 aataatttaa aattagaaga tgaaaaatta gtaaaaattg gagcattact ccataattta
    27421 agtccattag ctactatgga tagaggttat agtataactc aaaaaaatgg aaaagtaata
    27481 aatagtataa aaggattaaa aataaaggat agtatagata tagtattaaa agatggaaat
    27541 ttagaatgta tgatagataa aattgaaaat aaagagggat agtatgaatt taacctacga
    27601 agaagcttat aaaagattag aaagtatttt aagtgaatta gaatctaaaa atgctagctt
    27661 ggatgaatct ttaagcctat atgaagaagg tataagcctt tataaacatt gtaataagct
    27721 tttagatgat gctaaattga aaataagtaa atttaatcaa ttgggaatag aagaagactt
    27781 taaaatagag gaggaataaa attggaattt aaacaatgtc taaaagaaaa agcaagtttt
    27841 gtagaaaaag tacttaaaga atatatgcct aaagaagaag ggtatcaaaa aactgttatt
    27901 gaagctatga actatagctt aagtgctgga ggtaaaagat taagaccaat acttacattg
    27961 gaggcttgta aaatagttgg aggtaatgaa gatgaagcta ttccttttgc tattgcaatt
    28021 gaaatgatac atacatattc actaatacat gatgatttgc cagctcttga taatgatgat
    28081 ttaagaagag gaagaccaac aaatcataaa gtatatggag aagctatggg gattttagca
    28141 ggagatgctt tgttaaacta tgcttttgaa gtaatgcttg caggctcaat caataaagaa
    28201 aatccagaaa agtatctaaa agctataaat gaaattgcca aaggtgctgg tatatatgga
    28261 atgataggtg gtcaagtagt agatgtagaa agcgaaaata aacaaattga aaaagaaaaa
    28321 ttagattata tacatatgaa taaaactgct gcaatgatgg taggctgtat gagagcagga
    28381 gctactatag gtggagcaaa ttctgaacaa atggaagaaa taactaaata tgcaaaaaat
    28441 atcggattat cattccagat agtagatgat atactagaca tagtaggaga tgaagctaag
    28501 ttaggtaaaa aggttggaag tgatatagaa aatcataagt caacataccc atctctttta
    28561 ggtttagata aatctaaaga aattgcacat aatttgattg atgaagcaaa gaaaagtata
    28621 gaaaaattat ctgatgatgt agatttttta aagggattgg ctgaatatat aatagataga
    28681 gaatactaaa atatacagct aaaatcgagg agggaaaaat ggattttttt tcggagattt
    28741 ttaacaatgg ggctttggga ataagtctga ttgcttgttt cttagcacaa tttataaaaa
    28801 tatttactgg taaggaaaaa agaattgagt tatcaagaat acttatttca ggaggtatgc
    28861 ctagttccca tagttctttt gttacaagtt tagctacagt ggtaggtatt gaaaaagggt
    28921 ttaattctac tgattttgct ataattaccg tgcttgcctt aattataatg tatgatgcag
    28981 caggtgttag acgggctgtt ggtaaacaag ctactatatt aaatcaaatg gtggcagata
    29041 tacagcatgg aaagcatata gagcagaaga aattaaaaga attaataggt catacaccac
    29101 tagaagtttg gtttggagct ttacttggaa tagtaacagc tttaatattg atgtaaattg
    29161 aaaatttaca gaataaaggt gaggataata tgtataaata tttagataaa gtaaattctc
    29221 caaaggatat aaaaaatatg agtatagaag aaatggattt acttgcaaag gatataagaa
    29281 agttcttagt aaaatctgtt tcaaagacag gaggacactt agcttctaat ctaggagtgg
    29341 ttgagttaac attagcactt cataaagtat ttgatagccc taaagataag attgtatggg
    29401 atgtagggca tcaatcttat gttcataaaa tagttactgg aagaaaagat tgttttgtat
    29461 ctttgagaca gtttaatgga ctaagtggtt ttccaaagga gaatgaaagt cctcatgata
    29521 tttttgatac aggacatagc agtacatcta tttctatagc tacaggtatt gcctgtgcta
    29581 gggatataaa aaaagaaaac tatagtgtta tatctgtgat aggagatggt tctataacag
    29641 gaggtatggc acttgaagct ttaaatcaat taggatatat agataccaat atgatagtaa
    29701 tccttaatga taatgagatg tccatagata aaaatgttgg aggaatgtct aaatatctgt
    29761 caagtattat aagaaactca acagttgaga aaatgacaga tgaagttgat aaaattttaa
    29821 atgttacaca aacaggtgaa attttatcta agactgcaca tagatttaaa gataaactaa
    29881 tgtatagctt ttctcctcag gactgttcat tttttgattc attaggtata agatattatg
    29941 gacctataga tggccataat actaaagaac taatagatat attgagaaaa gctaaacaca
    30001 aaaaagggcc tgttctttta catgtaatta cgaaaaaagg aaaagggtac agatttgcag
    30061 aagaacaacc tgataaatat catggagtat caaagtttga tataaagaca ggtgttacat
    30121 ctgcaaaagt taagtctatg tcaattagtg ttggagaaaa acttgttgat atggctaata
    30181 gtaatgaaaa tatagtagca ataacagctg caatgccatc tggtacaggg ttaaacctgt
    30241 ttgaaagtgc atatccaaaa agatattatg atgttggtat agctgaacaa catgcaacag
    30301 gatttgcagc aggtcttgca aaaaatggta tgaaacctta ctttgctgtg tactcatcat
    30361 ttttgcaaag ggcatatgac caagttattc atgatgtatg cataacaaaa aagccagtta
    30421 cttttcttat agatagagct ggattagttg gaaatgatgg agaaactcat catggtatgt
    30481 ttgatttaag ttacttaaat tctattccaa atattgtagt aatggctcca aaagacacaa
    30541 gagagatgga gcttatgatg gatttgtcat taaaattaga ttgtccacta gcaattagat
    30601 acccaagagg aagtagttat tacctagata agggagagta tggagagatt gtactgggta
    30661 aatatgaggt attagatgac ggtcaagata cagtcatact ttgtattgga agtatggtaa
    30721 aacatgcttt agaggctaaa gaaatattat caagagaagg aataaatccg acaatagtta
    30781 atgctagatt cttaaagcca attgatgaag gtatgttaaa agcattatta aaaaatcata
    30841 aaaatgtagt tactatagag gataatattg taactggagg atttggaagt agaataaata
    30901 agtttattat agataatgaa tataacgtaa atatacttaa tatagcaata ccagaagaat
    30961 ttgtaaaaca tggcaatata gatgaattat atgactttgt aggattatcg ccaaaaagta
    31021 ttgctgataa gataaggaaa ttagttattg agtaaaaatc taatgtagat attgtaaatt
    31081 aaaatttata taaaatatag aatttatcaa ttagaataaa tatcaagtgt gttacttcaa
    31141 gctactttag ttatcattaa agtagctttt aacagaaagg taattgtatg aaaaaaagaa
    31201 tagatttatt attagtagaa caagggtact ttgaaagtag agaaagagcc aaaaaagcaa
    31261 taatggctgg attggttttt gttgataatc aaaggtgtga taaggctggt acagaagtaa
    31321 aagaagattg tagtatagaa gtaaaaggaa atccaatacc atatgtcagt agaggtggtt
    31381 taaagcttga aaaagcaatg aaaaactttg atttgactat tgatggaaag gtatgtatgg
    31441 atataggggc atccacaggt ggttttacag attgtatgct caaaaatgga gcaataaaag
    31501 tattttctat agatgttggt tatggtcaac ttgcatggaa attgagacaa gatgataggg
    31561 ttgtgtgtat ggaaagaacc aatataagaa atgtgacaat agaagataca aagcagtttg
    31621 ctgattttgc gtcaattgat gtgtctttta tatcgttaaa gttagtactt ccaaaggcaa
    31681 aggagttagt acgtcatgat ggggaagttg tcgctttaat taaacctcaa tttgaagcag
    31741 gaagagaaaa agtaggtaaa aaaggagtag ttagagaaaa atctacacat atagaagtta
    31801 taaaaatgat ttctgatttc tcagtcgaaa atggatttga gattttaggg ttagattttt
    31861 ctcctattaa aggtcctgaa ggtaatatag aatatctaat tcatttgaga aatggaaatg
    31921 aaggatatga atttgatggt gagacataca ataacaaaat agtagaagtt gttgaagctt
    31981 ctcataattt agacaaatag gttaaaaggg ggcttagatt tgatcctcga attatatatg
    32041 aaaaattgtg ccttagttga agaactaagg ttgaatattg ataaaaactt aaatatactt
    32101 acaggtgaaa ctggttcagg aaaatctata ataatagatg cacttggact ttgtttaggt
    32161 gataaatatg ataggtcttt tttaagaaaa ggaacagata agggacttgt tgaggctgta
    32221 tttttttcag acaatagata tcttaaaaaa atattagaag aaaatgatat aagtatggaa
    32281 gatgataatt tattggtaat aactaggttg atatactcag atggtaagag tactgctaga
    32341 gtaaatggaa ggactgttaa agtgtctctt cttaaagaaa ttgcatctac acttatagat
    32401 atacatggtc aacatcaaaa tcaagctttg tttaataaag atactcattt aaaatttcta
    32461 gacctttttg gagaaaatga attggaagag tttaaaattg cttataaaaa tgtgtaccat
    32521 aagtatagtg aagtaaaaaa agctttaaat tgcttgacag aaaataaaga tgagatgcaa
    32581 attcaacgag aaatagattt attaagattt caaataaatg aaattgaagc tgcaaatcta
    32641 aataagaatg aatatgaaga tttattaaaa caaagagaag tttatagaaa tagtgaaaag
    32701 atatataata atctaaattc gagttatagc aagttacata atggtgagta taatgttatt
    32761 gacctaatag gtcttgcatc taaggaactt aatgacatat ctaaatatga cagtgtttta
    32821 agtgaatata gtgatactgt agaaagaatc atgtatgagt tacaagacat atctggtgag
    32881 ataagaaatt acaaagataa tatagacttt gaaccttatg agctagagca gattgagctt
    32941 agaatcgacg agattaataa cttaagaaga aaatatggag acagtataga tgatatattt
    33001 gagtattatg gaaaaattaa agatagatta gatgaaatat taaacagaga tgaaagagtt
    33061 gaacagttaa gaagcaaact gatgaatata gaagaagatt taaaaataaa agccagtaaa
    33121 ttgactaaag caagaataga agtagcaact actttagaaa aaatacttct tgatgaatta
    33181 aaaagcttaa atatgaaaaa tgtaatgttc aaggttaatt ttgaaaaagg tccatttact
    33241 ttagatggag tagatgatat tgaatttatg atatctttta acttaggaga agatattaag
    33301 cctatatata aagttgcatc aggtggtgag atgtccagat ttatgttagc gtttaaaacc
    33361 atattggctg atatagatga tattgataca ttagtgtttg acgagataga tactggaata
    33421 agtgggatag cagcacagat agttggtgag aaattgagtg atatagcaaa gaaaaaacag
    33481 ataatatgta taactcattt acctcaaatt gctgcaaatg cagacactca ttattgtata
    33541 gagaaagata cttcaaataa taggacgttt accaatgtta gtaagttaaa tgaatctcaa
    33601 cgaaaaaatg aaattgccag acttatagca ggtaacaata taacagaaaa aactattgaa
    33661 catgcaagtg aaattattga gttggctaaa aaatgttagt gaaaaacaag cattttaata
    33721 ggtgtaaaga atttttatag aaatgcttta catctatttt ttatgatgaa attattttaa
    33781 aatatctagt gcctatattg aagaaaagta ggtttgaaaa tggaatgaac tttgtataaa
    33841 tttgtaactt tgaaatttct aaatgcttaa gtataatatt atttattaag gtagtagtga
    33901 ggggaggcat aaaatgaata aaaaagatac tatgcactta atttttataa ttgtaatttt
    33961 tattttatta tggtatattg tggtagcagt aagtgagatt aataaaaata ttgaaagttg
    34021 tagtaacaaa ttagattatt tgtcacaaga gcttatgagt actaagtcgg atattagtaa
    34081 tactataaat gaagaaatgt caaaaagcta tataacaaaa agtattgact taaaagtgaa
    34141 aaaaatcgag aaaaaagaat gtattattga tatagatgtt caattaagta aacttggaaa
    34201 aaatggaaaa gtatttttta tgtataaaga agatagtgat aaatggaatg aaactgagat
    34261 gacaaaacat ggagaattat catatgcatg tgaaattaag ataaatacag gaagtgaata
    34321 tgactataaa gtagttacaa gtggagctat atctgaaagt tctgatgtgc aaaaattgac
    34381 taaatctgat tatatgcctg aacaacccat atttaattct gggataagag ataatagaga
    34441 atactttatt gaaatagtag aaaatagtaa tcttttcaat catgagagtg aaaagtcaaa
    34501 aaataaagtt tatgataata ttagattaga aaaagtagat attattgtaa atgaaggaaa
    34561 aaaagacact atatacaaag ccaaattagc tgaaggctta gatgatggca aaactaataa
    34621 ttcaaataga aatcaagtga attatgaagt aagttttcct aaaagggata ttgatgacat
    34681 aatttatatt aaagctaaac ttacatacaa taatggagta gaatatacaa aggatataac
    34741 tgaagatata actatgggat tacaagattt agaaaaataa atacatattt aaataaatat
    34801 tcaaatagat ttgttcaata aaatagagta taaataaaac tgtagtagtt ttacttatac
    34861 tctattaaaa aattataatt ttaattccat atatctagcc ccttcaattg gattataagt
    34921 ataaggttct atatcataaa aaccaaactt ttcgtataat ccttgtgcac ttttcatgct
    34981 tggaagagta tctaatctca tgtgtgtata tccaaatttt ttagcttctt caatgatttc
    35041 ttccaatagt atttttccta tttttaatcc tctaaattga tttcttacat ataatctttt
    35101 taattcacat attttacctt caagtttttt taaagcaaca caacctgcca aattttcatc
    35161 aacaaatgcc aatatcaatg agcctgaagg ttttttatat tttcctggaa gtgtttttaa
    35221 ttcattatta aagtcttgaa aacataagtc tatatttaga gaatttgaat attcagtaaa
    35281 tagaagtttt acgttttcaa ggttttctaa atctttttct tcaacaaatt gtaaagttat
    35341 attttgagcc attttatccg ccattatata cacatgttta tataatgact tcacctcctt
    35401 gatatatgta tatttttctt aatatcaatt agattttaat accattactt tttcaccacc
    35461 atagtcaagc tctccattaa actcaaatcc aaaatcttta taaagcttaa tagccatttt
    35521 attgtcttcg aatatactaa gatatagttc atcacagtta tattcatttt taagatgatt
    35581 tacaagaaaa tctaatgatg cttttccata tcctttacct tgatattttg aatcaatcat
    35641 aaatctatca agccaaactc tatcatcttc taaacgacca tacatagcaa atcctattaa
    35701 agaattttca tcatatattc caacaggtac ccaagctgtt ataaatttag attcagcaat
    35761 agaaagtgca ttgctttcta tataatctaa ctgactttta tctacagaca aactaactaa
    35821 atcatgccaa ttatttatgt ctgcaattct tacatttaat ttcaagatat taccgccctt
    35881 caaacttttt gttacaatct aatactatca tattatgtat taaaaatcac ttattaagat
    35941 tttaaataaa ctttttaaat gaataaattc tcaggaaaaa ggaattataa atacataata
    36001 atacagcata ggagtagatg gatatatgaa caaaataaat aaaaatttca aaaatatttg
    36061 gataatatta ggagctgtat cgatttttac catggttata atttttaata aaaattgtaa
    36121 tgttaataaa gagactcaaa tagaagcttt tacaattaag aatcatgata aaaaatttgt
    36181 atatcctatg ggaaatataa ttggagtaaa agcaaataca gatggtgtat tggtcttagg
    36241 atatgaggaa gaggatgtag actatattgg aggcatacaa atcggtgata atatagtaaa
    36301 aatagataat aaaaggataa aaaatagtca agatgtatca gagattttga ataagattaa
    36361 aaaaagtaaa gttgaagtga cttttgagag aaaaaataag tataaaacag aagtaataga
    36421 gactaaaaaa gaaaatggca agtataaact tggattatgg gtaagagata aaatatctgg
    36481 aattggaact atgacttttt acgaccctag tatggaaaag ttcaaagcaa ttggtcatgc
    36541 cataaaagat tcggatacta atgaactatt aaagataaag caaggatata tatataagcc
    36601 agaacaatta aaaatagtaa aagctagtaa tgaaaaagta ggtaaaataa aaggagattt
    36661 taatgacagc aatttaatgg gtaatttctc aaataattca gagttaggta taagtggtaa
    36721 tattacagaa aaccataata aagagtttaa tgtagcaaat aaagaaaagc aattaataga
    36781 agttggaaga ccgcaagatg taaaaatagg aggtgcagta attctttttg aggataaaaa
    36841 caaaaatata acaagctatg atataaaaat agaaagtata gtatatgata aaggaaatta
    36901 tagagatatg gtaataaaag tagtagatga caagttattg gaatacacag gaggtatcgt
    36961 acaggggatg agtggagctc caataataca aaataataaa attattggtg caataactca
    37021 tgtttttaga gataatccga aaaaaggata tggtattttt atagatgaaa tgataaaatt
    37081 gtaggtgagg cattaaaaaa ttttattatt ttatcaatta tctaggagga atataatttt
    37141 ggagtgtcga atatgcttta gagtagataa ttaggaagca attgtgtaaa aagtttagtt
    37201 ttctgtaata agaagatgtt ttttaatggg gggattttta gtggaaaaaa tcaaaatagt
    37261 tttagcagat gacaataagg atttttgtca ggtattaaaa gagtatttgt ctaatgaaga
    37321 tgatatcgat atattaggca tagctaagga tggaattgaa gcattagact tagtaaaaaa
    37381 gacacaaccg gacttattaa tactagatgt aataatgcca catctagatg gattaggtgt
    37441 aattgaaaaa ttgaatacta tggatatacc taaaatgcca aaaataatag tactatcagc
    37501 agtaggtcaa gataagataa ctcaaagcgc aataaatcta ggagcagatt actacatagt
    37561 aaagccattt gattttgttg tgtttataaa cagaattaga gagctagttt ctaatagagt
    37621 tacacaagtt gagccaaaac ctagaccagt tcaagagact caaatgacta gaagtgattt
    37681 tgttaagaat gtaggaaata tagagacaga aatcacaaat ataatacatg aaataggagt
    37741 accagctcat ataaaaggat atttatattt aagagaagca ataaaaatgg ttattgataa
    37801 tgttgagctt ttaggtgcag taacaaaaga gttatatcca agtatagcta aaaaattcaa
    37861 tacaacacca agtagagttg aaagagcaat aagacatgca atagaggttg catggagtag
    37921 aggaaaagtt gacacaataa atcaattatt tggatatacg gtacacaata ctaaaggaaa
    37981 accaactaat tcagaattta tagcaatgat tgctgataaa ttaagactag aacatagtat
    38041 ggttaaataa acaagacata aaaagtaagg ctttttaatt aaggcattgg ctataaatgc
    38101 gtattacaag cagcgaaacg gtataaccac tagggttata ccgtttcgct attttaataa
    38161 atataaaaat tttatttatt atttgcttac tatatcaata taataatttt attatactat
    38221 ggatatagta tgtgtcttta caagttgtaa actgacagtg gtttattttt taatataaat
    38281 attgactttg atgcaggtaa actttgtatt tttaagcgta ttatggaata tgttaaataa
    38341 aaaaatgatg aaatatagta ttgtaaatgc caaagatgca aaacaaactt aaaacattta
    38401 ttttattgtt aagtaatgct ataatataat gtgattttaa taatgatagt ggaggtttaa
    38461 atatgagagt cgaggcccct ataaaagtag atcgaaaaac caaaaaactt gctaaaagag
    38521 ttgaaagtgg ggaaatagca gttataaatc atatagacat agatgaagtt gctgcaaact
    38581 ctttagtaga agctaaaata aaacttgtca taaatgcggc tccttctata agtggtaggt
    38641 atcccaataa aggtccaggg atattaactg aaaataatat actaataatt gataatgttg
    38701 gagaagagtt atttgaagaa ttaaaagaag gagaaactat agaagttgta gatggaaaga
    38761 tatatagaaa aggaaaattt ttaggtgcag gagaagtttt agacaagtat gaggtttcac
    38821 accaaataaa ggctgcctat gagaatttag ctgtagaatt ggatagattt atagataata
    38881 ctatagatta tgctaaaaaa gaaaaaggat ttatacttgg agaagtagaa attccaaaag
    38941 tgaaaactaa ttatgctaat aaacatgtat tgatagttgt aagaggtcag gactacaaag
    39001 aagatttaag tactatgctt tcttatatag aagaggtaaa gccagtattg gttggtgttg
    39061 atggaggagc agacgcttta attgagtttg gatatactcc agatgtgatt gtaggtgata
    39121 tggatagtgt aagtgatgaa gctttaaaaa aggctagtga aatagtagta catgcttata
    39181 cagatggaag agcaccaggt cttaaaaggg ttgaggaatt aggcttagat gcagttgtat
    39241 ttcctgcacc aggaactagt gaagatatag ctatgcttat cgcttatgag tataaggctg
    39301 agttaatagt agcagttggt acacattcaa atatgattga ttttcttgaa aaaggaagaa
    39361 aaggtatggc aagtactttt ttagttagac taaaaatagg ttcaaagctt atagatgcta
    39421 aaggcgttaa tttgttatac agaagtaaat taaaaataaa gtacatatgg gcattaatag
    39481 caacagcatt atttcctgtg ttggtagttg catcattgtc accaggagtt caacagttca
    39541 tacaactaat gcaattaaag tttagagtat tattacagat gtaaatttta ggaggataaa
    39601 tatgcatatt aatatgaaat attatatagt cactataggg gctatattta tagctttggg
    39661 tataggcatg ttagtaggat ttaatttaaa taataatcaa caattaagtg aacaacaagc
    39721 aaacataata aatgatttgg atgataagtt tgatatctta aaagaaaaaa atgataaact
    39781 tgacagtgac ttagcaagtg taaatagaga ttatgaagag gcagttaatt ttataaataa
    39841 aaatgtagat aagattttag ctggttcgct gaatggtaaa agtataggaa taatatctgc
    39901 taatgaaaat gacgattata caaaaaatat agaagacata ataaataaat ctaatggaag
    39961 cattgcattt aatataatat taaaagaaaa cataacaaat ccagaaaagt taaaggaaat
    40021 gtcaactaaa ctaggtacag atgtaaaaaa tgcaaatgat gcagttaatt atataattga
    40081 cactcttaaa aaggaagatg caagtgacat tttaacatat cttcaagaac tagatgtaat
    40141 aaagtttaat tttattggag atacatatct aaaatatgat tccgttgtaa tagctggtgg
    40201 aaatgatgca aaagacagta caaaacagtt tgaaaaaata gaaaaatttg tagtttcaaa
    40261 acttaaatca gagaataaat atttagtaga agttcaaaat acaggagtta aaacttctta
    40321 tgtagaacta tattctaaaa acaaagttgc aactatagac aatattgatg aaggtatagg
    40381 aagtatatct ctagctattc ttctacaaca aggtaatata gttggtaatt ttggtagatt
    40441 ggatactgca acttcattgt tgccatctat taaatagaaa taattttaag gagagaaaaa
    40501 atggattctt atataagtat aataatacca gcttataatg aagaaagtag aataaaatat
    40561 acattagaaa gcatattgga cattaagcaa ataaatgaaa taatagttgt ggatgatggt
    40621 tcaattgaca atactgctaa ggttgtatct gagctagaaa atgaaaagat taaatttttt
    40681 aagttagata aaaatagagg taaaggatat gctttaaatt atggtatcaa attagcaatg
    40741 aaaaatgcag atattatagg ttttctggat ggagatttag gaagttctgc taaagaagtt
    40801 gaaaaattaa tagtacctat attaaatggt gaggcggatg ttacaatagc caagtttcct
    40861 cctgcaaaga gaaaaggagg tttgggattt gtaaaaggtt tagctaaaaa ttctgtgttt
    40921 gagatgactg gtgttgagtt agatgcaact ttatcaggtc aaagaatatt taaaagagaa
    40981 gttttagaaa agtttgatga aatacctttt ggatatggag tagaagttgg aatgaccatt
    41041 gatatattaa agtatggatt taaaattaaa gaagtattag taaatatgac tcataatgaa
    41101 acaggaagaa atttaaaagg ttttattcat agagggaaac agtattatca cataaaaaag
    41161 gttttaggac aaaaaaagaa agaatggagg taatatctta gtgaaagatt atatacttta
    41221 taccatttta ttattaactg gattatttgg aacctatgct gtaattccac tatttaaaaa
    41281 tttattgata aatggaaatg tacttagacc aaactataaa aaagatatga taccagtaag
    41341 tatgggaatt gtatttttgc ctatgattgt aatcaatgga ataatacttg ctttctttac
    41401 tacagaattt aaggatttag cgtatatatt tatgtttatg tttggtatga tatccatgtt
    41461 ttttgcaggg atactagatg atgtcattgg aaatagagat gtaagtggtt taaaggggca
    41521 ttttaaaagt ttatttaaag gaagtcttac aacaggaggt tttaaagctt tatttggtgg
    41581 atttgtaggg cttatagttt ctgttgctat atcaaaagac ataattgata taatagtaaa
    41641 tacactaata atagctctat caactaattt aatgaatctt ttggatttaa gaccaggaag
    41701 ggcaataaaa gcatatttat tcataatggt aattatattt ttaacattga caggatttgt
    41761 tcaagttctt ccattactta tagtaccaaa tgtacttgca tactttaatt atgatttaaa
    41821 agcaagggct atgatgggag atactggttc aaacgtcctt ggaatatcta ttggaatgct
    41881 catttctttt gggtatccat ttaacattag attaggatgg ttaatatttg tagttattat
    41941 gcatatattc actgagaaat actcattaac taagataata gaaaaaaata aactattaaa
    42001 ttttatagat aaattgggaa ggtagtatta tgataagcaa aagagtgggg attgttgaat
    42061 caatagtaag tcaaactgaa acactagatg atataagagt aaatataaat ggagaaatac
    42121 aaagggcata taattatccc aaaatatctg gaactataaa tataggtgac gaagtggttt
    42181 taaatacaac agcagtagaa ctgagcttag gtacaggtgg ataccatttt gtaataacta
    42241 atttaaataa tatagaatct actttaactg aaggtggaca tataatgaaa cttagatata
    42301 cgcctcttca aataaaagtg gattctgttg aggaacaaga aagtatttat catgataaat
    42361 ttgcgaattt taaaggatta gaaggtcttt ctgttgtagt agggactctt catagtatgt
    42421 taactccatt tgcagcttca tttaaaagaa ataatccaaa taaaaaatta gtgtatataa
    42481 tgactgatgg agcatctctt ccaatttatt taagtaaaaa tgtggatact ttaaaagaaa
    42541 aaaaattaat agatagtaca ataactatag gaaatgcgtt tggtggagat tatgaatgta
    42601 taaatatata tactgcatta attacagcaa aagaaatact aaaagcggat gcagtatttg
    42661 tcagtatggg acctggaatt gctgggacag gaacaaaata tggatttaca gggattgaac
    42721 aaggctctat tcttgatgct gtaaaaaaac ttgaaggaag agcaattgca attccaagaa
    42781 ttagttttgc tgacaaacga gaaagacatc aaggtatttc acatcatagt atgactgtat
    42841 ttaaagaaat tgtaaatgta aatgtagaca taccaataac tatatatgat gatgaaaaat
    42901 tgaactttat aaaagagcaa atcaatttaa atggtttaga caaaaagcat aatataattt
    42961 atatagataa taataaaacg aaagaggatt tagactattt taatctaaaa gtaaagagta
    43021 tgggaagaaa ttatgaacaa gatgaggcat ttttcaaagc tgcaagcaca gcagcatatt
    43081 acttaatgga ggtttaagat ggtattagaa gaaaaaacta ttagtagtga tagggtttat
    43141 acaggtaaag taattacttt aaaggttgat actgttgaaa ttccagggca aggatatcaa
    43201 aaaagggagt tagtagaagt tggtggagca gtaggaatag ttgcaatcac agatgataat
    43261 aaagttgtgt tagttaagca atttagaaaa cctatagaaa agccaatctt tgaaattcca
    43321 gctggtaaac tagaaaaaaa tgaaagtcca aaagaatgtg ctgaaagaga attaaaagaa
    43381 gaaacagggt atagtgcaaa aaatattaag ctaatacata aatttttcac ttcagcaggt
    43441 ttttctaatg agataatgtt tgtatattta gcaacaggtc ttactccagg agagaataat
    43501 ttagatgcag atgagttttt agatgtctat gaaattgagc ttgaagaagc atataacatg
    43561 gtattaaaaa atgacgttga agatgcaaaa acatctattg gattattatt agtaaaagat
    43621 atgtttaaaa attaatattt ataaaatgaa taaccctttg tcctaaagca tattatttta
    43681 taaaggacaa gggggaatta aattgagaag aacatataga aaaaatgatt ttagagaatt
    43741 aaatagacaa ataattatta taggattgtt gtttatgatg tctattgttg ttgggtctta
    43801 cataaataaa atattgccag gttctagtaa caatatatta aacaatataa atcctgctgt
    43861 agagtattat aatctaaata taagcataaa agatacagtg atacaaaatt taaaatcaga
    43921 tgcaatcttt atgggctcaa tagctctatt aagtctattt gtagtaacaa ttccagcagt
    43981 gttggtagct tttgtattaa aagggatgtc aattggttat acaataaata gttgtatatt
    44041 ggcattaaaa tttaaaagta taaaaatgat tttaataata ttatttaaaa atttgattat
    44101 tattccatgt gcaatcatat tagcattaat tagtttaagt tattttaaag aaatggttta
    44161 tgaatttaaa aagaaaaata gaaaaaatat gcaattttta attaagaggt atatactaaa
    44221 tattataatt ataatagcac tatcattggg gttgcaatta atattaaata ctgcaagtat
    44281 tggtattatt aagttcctag ctaaataaag gagtttataa tggatattat agagggatat
    44341 atagattaca taaaagataa aaaaaaatta tcagagaata cagtggcatc atactttatg
    44401 gatataaaaa aatatatgga atatataaat caaaaaagca ttaaactagt agatatagta
    44461 gaaaatgata ttataagtta cttaataaag ttggaaaaag ataatgtatc aatagctaca
    44521 atagcgagga tgatatcctc tataaaatct tttcatgatt atttattttt aaatcatata
    44581 tgtacaaata atcctgcaaa ggatataaaa aaacctaaaa taaaaaaaga aaatataaat
    44641 atacttacag aagaagaaat tgaaagacta ttaaattttc caaagttaac aacacctaaa
    44701 ttgattaggg acaaggctat ttttgaagta ttatatggta ctggtataaa agtatctgaa
    44761 ttagtagaga tgaatataga ggatattgac ctagatattg attatatata ttgtaattca
    44821 tgttgtaaaa atcaaagagt gataccactt tgtgatatta caaaacttta cttagaaaag
    44881 tatctaaaag aagcaagacc taaaatggct gtagaaggtg aaaaatcatt attcgtaagt
    44941 tcattaggtc aaaggtttac acgacaaggt ctttggaaag tcataaaaaa atattctaat
    45001 ctagccaata tagataaaaa tataaatcct acaatgctta gacattcatt tgctattcat
    45061 ttgttaaatg aaggtgctaa cattgctgtt gtaagtaaaa ttttaggaaa tgttaattta
    45121 tctagtttgc aagtatattt aaatcatata gataaaaacg tgagaagaga aataaaggag
    45181 aaacatccta gaaatgatgt tgatgtagaa ttacaaaaag cagaagctaa atagactata
    45241 tagcaaattt tataatttag aaggagcata tttatatgag cagagtaatt tggattgtaa
    45301 ttgatagtgt tgggatagga gcattgcctg atgctgaaaa atttggtgat agtaaggatg
    45361 taagtaccct tggaaatata tttaaggaat atccagatat tcagattcct aatatgagaa
    45421 atttaggtat aggaaacata gatggtatag atttttttga aagtataaaa gaaccaatag
    45481 gttgttttgg aaagtgtaag gaaatgtctc agggaaaaga tactactaca ggtcattggg
    45541 aaatgactgg tataatagtt gacaaaccat ttaagacatt tgaacatgga ttctctaagg
    45601 agataataga agagtttgag aaaaaaactg gaagaaaagt agtaggaaat aaaccagctt
    45661 caggaactgt aataatagat gagtatggag aacatcaaat aaaaactgga gatgtaatag
    45721 tatatacatc agctgatagt gtatttcaga ttgctgctaa tgaagaagta attcccctag
    45781 aagaactcta taatatgtgc aaaatcgcta gggaaataat gatgggggat aatgcagtag
    45841 ctagagtaat tgctagacca ttcataggta agaaaaaagg tgagtttgtg aggacttcaa
    45901 atagaagaga ttattcacta gacccatttg aaccaacagt tcttgataat ataaaagaat
    45961 caggacttga tgttttagca gttggaaaaa tagaagatat ttttaatgga aaaggaatta
    46021 cagatgctat ccacactaaa agcaatatgg atggagtgga tgaaacttta aattatatga
    46081 aacaagataa taaaggtcta atatattcaa atctcgttga ttttgattct aaatatggtc
    46141 ataggagaga cccagaagga tataagaaag ctcttgaaga gtttgatagt agacttcctg
    46201 aaataatggc taatatgaga gaagatgata ttttaataat caatgcagac catggaaatg
    46261 acccaacata taaaggtaca gaccatacaa gagaatatat acctgttatg atttatggca
    46321 ataagattaa aaaaggtttt aatttaggag taaaagatac ttttgctgat attggagcaa
    46381 ctgttgcaga tatactaaat gtaaagttgc ctaaacatgg gtcaagcttt aaagaagatt
    46441 tattttaaac tataaaatga taaaaactta gcttaatagg atggagagat tggaatgttt
    46501 gaaaagatag cacaaagtag taaatttata aattctaaaa gtgatataaa acctaagatt
    46561 ggtttgatat taggttctgg actaggtgat ttagcaaatg atatagaaga ccctgtcgca
    46621 attaaatata gtgaaattcc aaacttccct gtatcaacag tagcaggaca tgctggacaa
    46681 cttgtaattg gaaaacttga aggaaaagaa gttattgcta tgcaaggtag atttcattac
    46741 tatgaaggat attctcagaa agaagctact tttccagtaa gggttatgaa agaattagga
    46801 gtcgagattc ttatagtaac taacgctgct ggaggcgtaa ataaagaatt taaagcagga
    46861 gatttgatgc ttataagaga ccatataaac tttagtggaa gtaatccatt agtaggaaaa
    46921 aatgatgaca gatttggtgc tagatttcca gatatgtcag atgcatattc ttctaaatat
    46981 gtagatgtag ttaaaaactg cgctaaagaa tgcaatatag atgtcaaaga aggcgtttat
    47041 atgtttttta gtggtccaaa ctatgaaaca ccagcagaag tgagaatggc acaaattttg
    47101 ggagctgatg ctgtaggtat gtctactgtt ccagaagtca tagtagcttc tcactctaat
    47161 ataggagtta ttggtatatc ttgtataaca aatatggctg ctggcatctt aaatcaacca
    47221 ctaagtcatg aggaagtaat agaaactact caaaaagtta aatcagaatt tatgaattta
    47281 gttaaaacaa cggttaaaaa tttataagtt aaagcttatt atagattttt tgaggaagtt
    47341 tataataaaa tagttaatgt ttaatttgaa aagaattaat aaaaagctgt cttgatatag
    47401 aaagttattt ctaataaagg acagtttttt tataatacta caacatttga aagtgtttta
    47461 aagtttaata tttatttaaa ttattatagg ttagaatgga gatttcaatt atgagaatgt
    47521 atgatattat aaaaaaaaag agagataacc tagagcttag taaaaaagag ataaactact
    47581 ttatagaaaa ctattcaaag ggagatatac ctgactatca agcttctgca ctattaatgt
    47641 cgatttattt aaataagatg aataaacagg aaactgtaca tcttacagaa gcaatgatga
    47701 actctggaga catgataaac ctatctgaga tagatggaat aaaggtagat aagcatagta
    47761 ctggtggtgt tggagataag actaccatag ctcttatacc tttggttgct tcatgtggag
    47821 caccagttgc aaagatgtca ggtagaggtc ttggtcatac tggtggtact attgataaat
    47881 tggagtctat accaggtttt tcgacagaaa tggagataag taaatttatt gattctgtaa
    47941 ataagtctaa aatagcagtt tgtggtcaaa gtgcaaaggt agcaattgca gataagaaaa
    48001 tatatgctct tagagatgtt acagcaactg ttgataatat atcattgata tcatcaagtg
    48061 taatgtctaa gaagttagct tcaggagctg atgcaattgt gctagatgta aaaactggtg
    48121 atggagcatt tatgaagaca ttagaagagt cttttgagtt agctaaatct atggttgata
    48181 taggtactgg tatgaataga gatacaatag ggattgtaac agatatggat gaacctttag
    48241 gttttgcagt gggaaactct ttagaagtta tagaagcaat tgagacatta aaaggaaatg
    48301 gacctaaaga ccttgttgag ctatgcgaag tattaggagc atatatgttg gttttatcaa
    48361 aggtagcaca tgattttgaa gatggtgttg aaaaaataag agaatcaata aaatctggta
    48421 ctgctataca aaaactcaaa gagtttatag agaatcaagg tggtaataaa gatgtagtag
    48481 atgattattc tttattcaaa aaatcaaagt ataaatatcc agttaaatca actaaatcag
    48541 gttatataag caaaattaaa gctgaagatg ttggtgtatc ggctatgatt cttggagcag
    48601 gtagagaaac taaagatgat attttagatt tatcagcagg gataatttta gaaaagaaag
    48661 taggagatta tgtaaatgaa ggtgaagttt tagcttacat gtattataat gatgaacaaa
    48721 agctttcatt ggcaaaggga agatttattg attcctactc tattgtagat tcaaaaatag
    48781 aaaaaaataa attaatatat ggtattgtaa ctaaagaaga gattaaaaag ttttagaaaa
    48841 ttttagaaaa gtgattataa aaggcctaac atgtcaaaaa taatgttagg ttttttattt
    48901 tttgatagac tataaattat accaaaaata tatattttaa aacatttttt acaatataaa
    48961 taagtttatt ctacaagatg attgaaaatt aaagtatcta gttttataat agaaaatgga
    49021 taaatataca taaggagtag attatattat gaactttaaa gaaaatagat tgaaaatagc
    49081 agtattaata attgttattt tgattatctt agcaggtata tttgtgttta tacaaatagg
    49141 tccttatgat aaaaataaca aaaaagatgt aattatcgac gttccaagtg gagcatctgt
    49201 tggaaaaata tctgacatat tatatgaaaa taaattaata aagaatgaac tattatttaa
    49261 acttttggta aaggttagca ataaagctcc gtctataaaa tcaggaacat acttacttaa
    49321 tcaatcgtat tcaaacaatg atataataag tcttttagtt tcaggtaaaa tatatcagga
    49381 tggtataaaa gtgactattc cagagggagc tacatctaaa gaaattatag caatgttagt
    49441 aagtaaaaat ttaggagata aagctacttt tgaaaattta ataaaaaaac ctcaagaatt
    49501 ttatgataaa tttccatatt tgaaggaaga tggaataacc tctttagaag ggttcttgta
    49561 tccagaaact tattatttta attcaaagaa acaatcagaa gaagatattt taagcgaaat
    49621 gttaaaagta tttgacagta aatacacgga caagtttaaa aagaaacaaa aagaattaaa
    49681 tatgactctt caagaagtaa tggaaatggc atcaataatt gagaaagaag ctgttcttga
    49741 caaagacaga cctataatag caagtgtgtt ttataataga cttaaggtag gtatgcctct
    49801 tcaatcagat gctacaatac aatatatatt tgaagagaga aagaaaattg taacatatga
    49861 tgatttaaaa attgattcac catataatag ttataaaaat aagggattac ctcctacacc
    49921 aatatcaaat cctggtataa aatctattga agcagcatta tatccagaga aaacagacta
    49981 tttatatttt gtagctaaga ttgatggtgg aaataattat agtacaaact atcaagacca
    50041 tttaaaatac gttaaagaat ataaagaagc aagggataaa caatcaaaag atacaaaagc
    50101 aacaaataaa gaaaatacaa aaaaataaac catgattata aaaagtctaa cagggtaaga
    50161 ataatgttgg gcttttttat tgaaaattgt tattcttaga tttataataa agaaagtgta
    50221 aatatatata acaatatgaa tttaaaaaat aataaattaa tataaaactt gaatatttat
    50281 tattttaggg agtgttttta tgagtaatat agttaatgat ttggttgaag attatataag
    50341 agtgacatta aaagaaaaag aaggattttt aaaagattta gaaatttatg cagaggaaaa
    50401 tagtgttcct ataatacata aagaagtttc agatttatta aaagtcttgt taaaggttca
    50461 aaaaccaaag agaattttag aagtaggatg tgcaattggt tattcttcta ttttttttgc
    50521 aactattata ggaaaagatg cagatataat aactgttgag agaaatgaaa aaatgataga
    50581 aaaagcaaaa gaaaacataa aattagctgg atttgacaat aacataacta tactagaagg
    50641 agatgcagaa gaaaaactta gtcaagtcca aggtgaattt gaccttatat ttatagatgc
    50701 agccaaaggt caatataagc tattttttga tttagttata gataaattaa aagatggggg
    50761 acttttagta tctgataata ttttgtataa aggtatggtt gctcatgatg attttgttgt
    50821 aagaagaaaa aagactatag ttaaaagaat gagaaattat ttagattata tatgtaattg
    50881 tgactattta agcacatcat tggtaccaat aggtgatggt gtagcactaa gttataaaga
    50941 aagtaaaaga ggtgatgtag atggaattaa ataaagtaga acttttagct ccagctggtg
    51001 atttggagag attgaaaata gctataacat atggagcaga tgccgtttat ataggtggag
    51061 aaatttttgg aatgaggtct gcagcgaaga attttagtaa ggaagatatg gcagagggag
    51121 tagcttttgc acatgaaaga ggcaagaaag tattcgtaac agtaaatata atacctcata
    51181 atgaagattt tctacaatta gaagactatt tgttggaact agaagagata ggaatagatg
    51241 cagttataat agctgaccca ggagtgctta gtgtcattaa aaaagtaatt ccgaatatgg
    51301 aaatacattt aagtacacaa gcaaatacaa ccaattatct aagtgcaaac ttctggtacg
    51361 agcatggaat aaaaagagtt gtagtggcaa gagaattatc ttttgatgaa atatctgaga
    51421 taagagcaaa aacaccttta gatatggata tagaagcgtt tatgcatggt gctatgtgta
    51481 tttcttattc tggcagatgt ttaataagca actatatgac tggaagagat gcaaacaaag
    51541 gttcttgtgc tcaatcttgt agatggcaat atcatttggt ggaagaaaaa agacctggtg
    51601 agtattttcc aatatacgaa gatgaaaggg gtacattctt ctttaactca aaagatttat
    51661 gtatgataga gtatatacca gagttaatca aatctggtat aacaagttta aaaatagaag
    51721 gtagaatgaa gactgcatac tatgtagcta ctgttgtaag agcatacaga atggcaatag
    51781 atgaatttta cagagaccca gaaaactgga agttcaatcc aatgtggatg gaagaactaa
    51841 aaaaaggtag tcatagacat tttacatcag gtttttactt gaataaacca acaacagaag
    51901 accaaaatta tcaatcagca tcatatgtta gaaactatga ttttataggt atagttagag
    51961 aaacagaaga tgaagatggt cttattgttg ttgagcaaag aaataaaatg tgtgttggtg
    52021 atgaaataga agttatggga ccatataaag aaactatgtt tactaaaatt gaagaaatgt
    52081 acaatgaaga aggagaagct atagaatcag ctcctcatcc tagacaaata gtgaagttaa
    52141 agctatcagt aaaagttggt aaagattata tgcttagaaa agttatagaa gaaaaggttg
    52201 aagaataata aaaaattgat aaaaatagta ttagcccctt agaaattgtc aataatgaaa
    52261 ttatctaagg ggtgatttca tgtcaaaaaa gaagactcca tttttgaaaa aagtgggaaa
    52321 gagaagctgg tgtatattta ctataatttt aataatttat agtgttttaa tttataggct
    52381 agttgatata caagtattaa aaggtgataa atacaaacaa agtgttgaat cacaaagcgt
    52441 tgagaaagtc gagttaaata gtggaagagg aataatctat gatagaaaca acaagaagct
    52501 tacagataca agtaaatctc aggtattaat tgttgaaaaa gaaaaattaa ataataacta
    52561 taaaattttg gagcttatta agaaagctac taagatgaat gatttagata tatataaagc
    52621 tgtacaagag caattgacac gccctataat acagattcag actaaaaata tcgataagag
    52681 tatgaaaaaa gaacttgaga aaaatggaat aatggtagaa gaaaaaacta tgaggtacgc
    52741 aaaagatggt ttattatcac atacaatagg atatataaaa gaagatgata aatcaggtca
    52801 atcaggaatt gaaaagagta tggatagtgt acttagaaat tcaaatgaaa agtatataag
    52861 tgcttttaaa gctggagatg caggaaatga aaaatctcta aacatactaa agggaagtgt
    52921 aaaaactgtt gataataagg ataaagatag acatctaaaa actactattg attataacat
    52981 tcaaaaaaaa ttagaacaaa tactaaataa agaagaaaat cctacagcag caataatctc
    53041 ggaagcatct acaggtgaaa ttcttgcaat gtgctctaga cctaattttg accaaaatga
    53101 catatcaaaa agtttaaaag gaaaaaatgg agaatttgaa aatagagtta taaaagcgac
    53161 atatccacca gggtctgtat ttaagatggt agttttattt tctgcacttg aaaatggtgt
    53221 tatagatgag aactatactt acaactgtac aggtaagact aaagttggga atactaatga
    53281 aatactgcga tgtaataaaa gagatggtca tggtttccaa aacttaagac aagctttttc
    53341 taattcatgc aatcctgcat ttttagatat agctatgaaa cttggaaaag aaaagatact
    53401 caaatctgct gaaaaattac atttatttga aaaggttgat attggtcttg atgaagaaaa
    53461 aattagagaa gctccaaaaa acatatcaat aagaaattta gctataggtc aagaaaatat
    53521 agaatttaca ccacttcaaa taaatcaaat gactcaaata atagctaata atggaacatt
    53581 caaaccacta tacttatata aaagtcttgt ggacaataat atgaatacaa taaaaactta
    53641 taagtcttca aaaaaagaag aacttatttc tccttatgtg tgtacacaag ttaaagaata
    53701 tatgaagtct gtatctagga ttggtacagc taaagaccta aaagatattg aaggtggttg
    53761 tggagttaaa actggtacag ctcaaagtag tttaaataaa aaagctatag accatggatg
    53821 gataacagga ttttatcctg aagaaagacc taagtatgta ataactgtgt tagtagaagg
    53881 aactcaaaaa ggaaataaat ctgcaacacc tatatttaag gaaatatgtg aaagtataaa
    53941 ataaattatg tactataaaa tttaaggata tatcgtaact ttgattttag atagaaaatt
    54001 ttgtctaatg atgtacgata tatcctttta aaatatagta ttaactttct tgaacaagct
    54061 ctttttctag ttttttaaga gctttttttt cgattctaga tacatatgac cttgatatat
    54121 cgagtttttg tgcaatttct ctttgtgtct tatagccata aggagttaac ccatatctta
    54181 actggacaat ttctctttct ctaggagtaa gaattttgtt tagttgttcg taaagttttc
    54241 caacttgaac ttttagttca acttcatcaa gaacatagtt agcctctgta ccaagtatat
    54301 ctaaaagact tatttcatta cacactactc tttatatgtt attggtatta ctggtcttca
    54361 tgtaatttca aatggaccat taattcaaaa gaatctagtt tttgttcttt agacttataa
    54421 taaactgctt tatcaattac agattttagg agtttatttt ttccaaggga atctgaatca
    54481 tggtaaagtg ataacacagt tttaatatct tctattattt tagaattgtc ttttttaaca
    54541 gtacttaatc tattttttac tgtcttaatt gaatctttaa tttcttcagt tcttgaactt
    54601 atattgttgg acctttcaat aaatgtatct tcatcatata ttcctcgttc tagtaaatca
    54661 tataacttat ttttttgctt gcctaattct tttaattcag tttctaagcc ctttaagact
    54721 cgtttttgaa attctatatt actatcttta ttatttttat ctaagtctat atcagatgct
    54781 tttaattgat atttttttac agtgtcttct aaagatttta aaatggcagc ttcaataaat
    54841 tcaaatcgag aacttttatt gcaaccagga tgataacaaa ttatatagtc gtggtctgca
    54901 tatggtctat atactaatgg tctaccacaa tttttgcaaa tcactattcc tgcaagtgga
    54961 ttttttattt gattaccata gctgacatga ccattttttt tgagtatatt ttgagctttt
    55021 ttccatgtta tttcacttat taaaggttca tgctttcctt ttgcctcaat ccattcatct
    55081 ttaggtctta gtttaactgt ttttatttta tttggatttt tagatttttt gtagtccttt
    55141 ttttgccact gaatgtagcc acaatatacc ttatttttta ttatatttgt aattgcagaa
    55201 ttgtaccaat tagcacctgt agcagtttta atgcctaagt tatttaggta ttttgatatt
    55261 ttactacaac ccatatcttc attaatatat aagtcaaaga tagtcctaac tacatcagca
    55321 tccttatttg gtacaagtat tctttctttt ctaccagttg ttacagcatc atatccaaat
    55381 ggagcagagg ttcctatgaa attcccttct tcaactgatt ttattctacc tctttgcatc
    55441 cttcttgata taagttttaa ttcttttctt gccataaatg cttcaaattc tgaatactcc
    55501 tcatcaaatt catctgttag gtcgtatgtt tttctaggtg tgataatttt ggtcttactt
    55561 tctttgaatg tttctaatat gataccttgg tctttcatgt ctccacgacc aagtctgtct
    55621 aagtccatta ctaagactgc atcatattta ttttcttcaa cttcttttag aagttcaagc
    55681 attttgggtc tatgtattat actttcacca gaaactaatt cttcttttat ctcaattaca
    55741 tctaagtttt gttcttttgc tagttttagt agggttgatt tatgtctact taaagtttca
    55801 aattcgcctt gtttttctgc ctcttcatca gcacgggatt ttcttaagta tatggcaaca
    55861 ctcataataa gtaatcacac tccttatgca ctattatata gtaaatcaat agagtttaaa
    55921 atatagaaaa taagcatgtc aaaagttaat ttaactttca acatacttat tttctataat
    55981 attaatgcct tataatcctt taatatcatt tagattagtt tttgcatatt cattatccgg
    56041 gttaagttca agcgccttat tgtaagattc tctagcttta ttaaattctg atttactcat
    56101 aaaaataaca cctcttaaat tgtaagcatc actagcgtct attttataag cttcattatt
    56161 tttatctaga tattctaagt atttatcaat tgactttaaa gcctcatcat atcgttgtaa
    56221 ctctatataa atatatgatt gtgtataata tgcaagtgga tttttatcat caatttctat
    56281 agcttttttg atatcaatag cagctttatt ataatcctta tcttcatctt tttctgtttc
    56341 tgacctagcg attttgacat aattagagct aagagctctt ttaaagtctt tatgccataa
    56401 taatttatat tcattatctt cgattataaa atatctatca aaatttgcag tatcttcttt
    56461 attttcagtg atatacttta tagtatcagt ttcttttatt ttaacaagat ttgtgtactc
    56521 tttattagaa tatttgtatt tctctacttt attttcttca ccaagtttat aatcttttag
    56581 ggtttgaagt ttaaataaag tatcttgata ttcttcaaat ttttctagac tgacgctttc
    56641 cttactgtct ttacttaata aattatatgc tacttcgtag tctttgattt ttatactatc
    56701 aaggtacttt ttagctactt ctgtagcact ttcttttgga gcacatccaa ccattgatat
    56761 cataattaaa aatgatacta atattgctgt tattttttta ctgattatca taatataatc
    56821 ccccttatat tgtctctcat taattttatt ttatcaccat attctaccaa agtcattaat
    56881 ttaagagtga tatttttcat taatattata ttaagtaata ataacctttt aaatttatgg
    56941 taacattttc ctgaaaagta ttatataatt taggtgaaaa tatttaagag aagtagatat
    57001 tatgaaaatt aaaaaattat taacaatagg gttatcttta tctatattta ttgctagttg
    57061 ccctatatct gcaaatgctc ttgataaaat tgaaccaatt aaaggtgcag ataaatacga
    57121 aactgctggt ataattgcag acaaataaaa ctatactaca gctatattaa ttaatgcaga
    57181 cagtacaatg tctgatggat taagtgcaag tggacttgca ggagctataa acgctccaat
    57241 tcttttgact aagaaaaata acataccaaa tgctacactt aaaagattag aaaaggcaaa
    57301 aaaagtatat ataattggag gagaaaactc tattgataaa tatactgaaa ctgttctaaa
    57361 aggcaaagga atagagatta aaagacttca aggtagtgat agaattaaga caagctataa
    57421 tgtcgcaaaa gaaataaact caattaacaa agtaaataaa gtcatattga ctaatgcttt
    57481 taaaggagag cctgatgcaa tgagtgctgc acctgtagca gttagagata aagcagctat
    57541 agtattaact gatggaaaaa gtgtccattt aatacaactg gtgtagaaag ttatgtaata
    57601 ggtggtactt catcaatgaa tgacaaaata gttaaggata ctaattcaac tagattaggt
    57661 gggacagata gatatgatac taacaaaaag ataataaata aattttatag tggagtaaaa
    57721 gaattttata tagcaagtgg tactgattta gtgtatgctc ttgttggttc taccattgct
    57781 aaaaataatg ctattgtgtt agttggtaat gatagtaata aaagtgtatt gaaaagtaca
    57841 actaaattaa ctgctatagg aaatttgagt gatagtatat tacagcagtg tttaaatgta
    57901 acaaaaaata taggcgattc taatactgga agagaatata cagtagaaga tgctgctaat
    57961 gtagtgaaaa aactattagg tgtgaagaaa ttttattacg aacctaatta ttcagaagat
    58021 gaagatttaa cttacaatct atagagtgga gttcatgtac aaaagattat cctcccaacg
    58081 attcgaaggg aggtgagttc atggataact ttttgtttaa tgttttagct agtttaacag
    58141 ctagtgtggt agtttactta atcagtaaac tattcaaaaa agcaaaaagc cactctcgca
    58201 caaagagtga cttacgagtt gaatttaaat ttatatttaa atccaaaaaa taaactctgt
    58261 ttatgatgaa ctccactcta acgcaaaata gattgtagtt cttcttgctt ttattatacc
    58321 acaaaacatt ataaataaaa ctgttgttta taaaaaaatg caaagtaaaa ttttttataa
    58381 acaatgataa aaattttatt atagagctat taacttatga aatatctgta aatgagcaga
    58441 tattttataa gttaatattt attgtttaca taaaatattt ggtaaaaata agtaagtttt
    58501 atatgttata atagttgtag caagaataat aatctaaagt ggcaagcatg tcagctggtg
    58561 ttatatcagt ttatatttat gataaaataa aaatctccca aacgccaata agaatgattt
    58621 aaaaaaataa tattttcact taaaaataat cactctttat aggagtaaat tattttcttg
    58681 attttattat actacaaatt ggtacagata tttaaaaata atatattcat aatattataa
    58741 atagttttgc agtgagcgat atttttgata aaatagggct taacagttga aacataaggc
    58801 attgagggta tatgataaat attatcattt gcactactcg tggttcactg caaatttgag
    58861 agaattgtat agatgtaagt gttggaaata ctcaatttat tttggggttt tatattaact
    58921 aagtggtatg taaaggcaat aaaacctgct attcttggta gaaattcatt tggttttata
    58981 ttaactaagt ggtatgtaaa gtttgacgtg ttaagtgata tagatagaag aatatgtgag
    59041 ttttatatta actaagtggt atgtaaagtt tgtttctctg taacatgatt atcattcata
    59101 attaaagttt tatattaact aagtggtatg taaagagagg ataaattgtt tacatagtac
    59161 cagttgggtc ttgttttata ttaactaagt ggtatgtaaa gttgcacttg aatagttctg
    59221 aagaacttaa gaactatggt tttatattaa ctaagtggta tgtaaagagc tttacaatat
    59281 caaagaaaaa ttgaaacatc tatgttttat attaactaag tggtatgtaa agggaaaatt
    59341 atgggaaaag tttacagata tcatgagagt tttatattaa ctaagtggta tgtaaagtaa
    59401 aaataaacct cctaaacaaa tatttcacgc atagttttat attaactaag tggtatgtaa
    59461 agcttgtaat cgctgaataa agatttctag cttcgttatt gttttatatt aactaagtgg
    59521 tatgtaaagg actctgccat ccctgtaacg aacagcctta cagcaagttt tatattaact
    59581 aagtggtatg taaagactta tctgtaaccc ttatatgttc aaattcatag ccggttttat
    59641 attaactaag tggtatgtaa agtatttcta gcgacttagt cggttgctcc atttagcgtt
    59701 ttatattaac taagtggtat gtaaagtagg attgacacgt tacgaccgtg caacgttgga
    59761 ctggttttat attaactaag tggtatgtaa aggcaataat attatcaatt ttaatatttt
    59821 gtttctcatg ttttatatta actaagtggt atgtaaaggg ttctggcaag tcacttcatg
    59881 ttgctagaga tatttgtttt atattaacta agtggtatgt aaaggttttt tcttcgtaac
    59941 tttgagcctt acatccacaa agttttatat taactaagtg gtatgtaaag aacaaaaaag
    60001 atacatctgt aactaatttt tatactgttt tatattaatt aagtggtatg taaagacaga
    60061 tataatgtca aaataaggtt aggactttta aaagcatagc catatcagta aaaatttaat
    60121 tatcatttaa aaaataaatt tttatttaaa gaatacacat aaatatttcc agactcaact
    60181 ctcttaaact attttcatat cttttagtag atacctttgt tgttttttaa tctttagcaa
    60241 cttgttcttg agtgaacctc ttattttttc taagttattt caagcttttc aaaaaattct
    60301 ctatttatat tcatcacaca tataatacaa ttaattcagt tttaattgcc aatatttagt
    60361 ttttctgtat ctgataaacc aagaatataa tcagtatata aaccaaaaat tttagcaaat
    60421 attattaacc catcttctct tgttggtctt tcaccagact ctattctatt cataacactt
    60481 gtatttatat ttgttttttc aagcaattct ttttgagaac tattcatatt ttccctaata
    60541 tatttaatcc tttgatttac atccatacat aattatctcg aaaagtttct gctttggaaa
    60601 tattgttatt gaaatataga aattaatgtg aatatatgaa aaagatacac tatgatttta
    60661 taaaaaagaa gacatacata aaatttatac ttacaaagtg ttgcaaaaga gttaggtttt
    60721 aaaaatgcat caacttattt taagtacagg aaagggtaat attcaattag agttgatata
    60781 ttacctataa actatctaag atacttgatt atgatataga aaatttaatt aagaggatga
    60841 taaggatgaa taatttacaa gtaatataaa gaaataataa gagaatttta aatacatagc
    60901 aactagcaga tatatatgaa aatgatgtaa acaatatata aactagcttc aatagaaata
    60961 aagagaatgt aaattatgtt ttattacaat tcgagtattt gaaagaattt aagaactaac
    61021 ctactaatag tcagttgatt tcaaaacatt ctagtcaatt atacatttgg accgaaagat
    61081 gtgcaaatag acactgcaaa atattagata ttgataaaac ttgagaacaa tttgacaatt
    61141 tagagaaaat atatttcaag attaagcgag agaaactaag ctgtatagaa gatgtattaa
    61201 tagaaaattt aatatttaag gatacaaatt aatcaagcaa taatatggca ttagaggtaa
    61261 aaatagaagt taaaataata aaagatgttg ttatattaaa tataacacat tggaaaaaag
    61321 aaacaagtct attaacagtt aaaattgcta gaggtcaagg tgatttaagt tatgtaaaat
    61381 attttactaa tgagagctaa gagcttctta acaagagttt tggtgtgtct attgaaataa
    61441 tgttaacaat taataaaaga cgaatggcag aagaaggcgt atgtaagttt aaaagagata
    61501 agttaaacaa actagatact atataagagg ataagaaact tatagaagaa tatgttactt
    61561 ttgtaaagga tttagcttta atatatagaa tataaagtga ttttaagtaa aaattgtaac
    61621 aattaaataa gtataagaca agtttatgag ttcatatttt ataacaaggg gtgatgttat
    61681 gaaaatacta caacaattta aaccatacaa atatgataaa gaaaaaaata tagaatatga
    61741 tgaaaaaatt atagctaatc aaaacacaga agtttatata gtatatccac gtatatctga
    61801 tgaagaaaat aggagaagat gggaagagtt caatgaagta gctaaagaag tagctattaa
    61861 tatagctatg aagaaagttg agacagtaga aagttaagag aatagagaag ttgctaatac
    61921 ttagcacttt ctcatagaag aaaattggac aactattttt aaataattag gaataaaaaa
    61981 gttaaatcat atttgaaaaa aggcgataaa attgttgagt attgttatta gtaataagaa
    62041 tgtattaact atgtattaaa agaaattata agtttttagt aaatatagta tttaaggata
    62101 ttaaaactgg taaaaaatat catttagact attaaataaa aaagtgctgg tcaaagtaag
    62161 caacacaata aataaaaaaa cttatagaaa ttataagata aaaagatgag atttgaaatg
    62221 gttgaagtta atataaaaat taaaggtgaa gtaagtgaaa taattgactc aattagaggt
    62281 atatttaata gctttgatga agagttgata ttagatataa aatcagagta agaatataaa
    62341 ggtagatacg tagaaagtta taagtaattc aagacttaaa ttaatttcta gtttttttaa
    62401 tgcaaaaatt taataaattt caaaatataa taattctgac atctaaaaag agctattaaa
    62461 aatgaataat attgactatt aagatatgaa tagtgtacat atattaaatt aagatatcca
    62521 gtaggttaat tttatataat gatggggctc aaattgaaga ggcgatataa aaatgtatta
    62581 atatatataa atataagaac tataataaat attaactgta aaattaaatt tatagagtta
    62641 tatttaacat ttaatgaact gaaaaataag ttagcaaata atgaaaaaac aacagtaata
    62701 aatgagaata acaaagcatt ttcaggatta gtggattttt ctaattgagc tttaatccta
    62761 gtttgttcaa tttcgatatt tctaatatta ttagtattat caatttattt gtaataatta
    62821 tcagtttttg cgaaatattt ttgataattt atagaacttt atatattttc cttgaagtat
    62881 ttttcgccct tattgtttgg tttccaattt taaaactcat aaactcccaa taaattcatg
    62941 tataatatac cttaatttat aatatattta agagtttatt tcagtaattt ttcgaacgat
    63001 tttgatatat ttcatttata gttttcaaaa acttatacaa aaaagagtgt ttcatttttc
    63061 taaacactct ttttataaga atttgaataa taatttttta aaaattgata atatggttat
    63121 ttctttttca agtttatagt ttcctcattt ccattccaag atataattag cttgtaacta
    63181 gattctctat ttgaataaag acctggtctt tgtattttta ttgctttttc ttgacctgct
    63241 tctaaaattt cattttttac aactacaact gcatcttcac catctataaa ttgataggaa
    63301 taattaaatt tttgttcaac tacatcttta ctatttttat attttatttc taattttgag
    63361 tgtccttcag tagttacaaa tttcgctttc caattttctg attctccaat aaagacattt
    63421 ttatcatttt ttatattaag tatagctatc acagagatta ttaaaactat aagaatacct
    63481 ataaaaaatt ttttcctcat atcaatccct cctttgaata attataacat gagaatagtt
    63541 attcaatact aattttaaaa atattgtgga tttatattct attacaatag aatgtctgtt
    63601 ataacaaata aaaagtaggg attaatatga attataggtt aaaaaaatct gtaacaacta
    63661 ttttatcttt actcataatg acaatactat taccaacaaa tgcaagtgct agtaaggtag
    63721 aagattcatc tatgaaagta atgtattcag agaaacaagt aacagacatg gacaaattat
    63781 acgatatggc taaaaatgga attactgatg ttaaaagttc caatgaaaaa ggtataatca
    63841 aaaatgcaag aacaggagag tccatatacg ttgattctat atcaacaact caattattag
    63901 aagttaaaca atctaaaaat ttaacatcaa agatatattc taagacagtt ttttgatact
    63961 aatacaataa agcctcttag aaaagcaagt atatcagaag atggatggga taaaactggt
    64021 ggtgtaaaag catctggtac agttacatat acaatatcaa aaggagatgg agcatttcaa
    64081 tatatttccg ttagtaaagt ttctggaaac tggtctattg cagacggcca gattcgtgta
    64141 acaaatagaa gggtgcatat aaatcaaact ggatttattt ctaatgggct aggaattaag
    64201 cagcaacaaa agaaagagcc aacatcaaat tctttctcat ataatgcacc ttctagttgg
    64261 aaaccagtag gtagagattc agttcaatac aattgtgggt ttgaatcaat tgcaacatta
    64321 gtaagaggag gaagttcttg gtcattcaat ttcattgttt aaccttaaaa tattttaagt
    64381 gaattttatt aaaattaaag atatacatat actttaaaat tagaaaatgt aaattaattt
    64441 gtgtttttaa agataattcc taagttatag caacaattgc ttatttacta ttaatatttc
    64501 ttaatatatg aattttattt taacaaatta taaagaatgt atattgggat gtttttaaat
    64561 atttttggaa attatatata ttataacaat tatagcaaat ttttagggta taatttttaa
    64621 aattatattc tactatcaaa agatatgaat aaatgattgt atatattata tcaattttta
    64681 tatttcatag tccatttttt catcacattt tcgcttgtca agaataatat ctttaagagt
    64741 gaagcgtttg atgtaaatct ggactttgtt tttgagacat ttttgaaata tttattaaat
    64801 ctttcgatta tacttgtaat atatattata gatattactt attttagtaa aactaaagga
    64861 tgatgctaca acattccagt tatttcccaa aattttaatt tctgaagtat actgtttaca
    64921 ttatttttaa tctattttat ctaaagtcga taaggcttct tattcattaa ctgacctata
    64981 tacagattta aaattactta ttaattcttt tgagtccttg tatgaaacat atttaaagta
    65041 tttcttatct aatgtattaa acatttttgt attacagaat gaggataact tgtagctatt
    65101 gtctctttga aataagtcat ctgtgtttac tcctaaaact acttatatct gtagatttta
    65161 taagttctcc atcttatata atttctttga ttaattgttt tcttaaatta ttcataaaaa
    65221 aatctccgtt acgatatttg attatattta aatcattgct ataaagaaga cttttattaa
    65281 ctttgaaaaa caaattattt taattgctgg tgattagttg ctaccttctt ttgatattaa
    65341 ttctattaga ttttatatag ttatatatct ctttctaact aagtaatata atattactgt
    65401 tatagtagct actaatatta ttgctattat tgtaatatct aatctttttg taaataatcc
    65461 tattgctata tattcaaaac ctgttacata tcgataaagt acttctagtt taatagttct
    65521 ttttctatct atatttccat attttgattc taatttatta aaatattttt cattacaaaa
    65581 accaatttta atttcaatta aagatattat tgtagttaat attataattc ccaatcccat
    65641 aagtataaga taaatattcc taaaattcat agaagatacc tcctttataa tataatcatc
    65701 aactcatgtt gtattaattt tcctaatcaa agtataacag taacacaagt ataaagagta
    65761 tctaaaatat tacaaatatt aaatgagaaa aagatggtaa gttaacccca ccatcttatt
    65821 ttttatctac taatgaatga tgctacaaaa gttagtataa aaaatgaaac aaacaatata
    65881 atgaacttgt gtaatttact tagttggaat gctttattac aaaataataa aattaatgct
    65941 gaaacaattg caaaaagtag atattttata atcattttat cacctattga tttctacaga
    66001 ttgtaagtag tatttggtta cacaagcacc tcctttatta taacgtattt tttaaattta
    66061 aagatttttc cagatgttat atttgactca aggtacattg taattaatct aactataagt
    66121 ggcaagtact gtcctatacc aggaatagca ccagccaaaa gtcctgaaat ccctatacca
    66181 gcatcagtta aagttaatgc aactccttta gaaagatata aattaaaacc tattctagcc
    66241 caaactattt tatttatttc agcttttcta taatataatt caagtagtgt caatatcgtt
    66301 cttcttaagg tttttaatta gcttatatat tgttattgta taatataatt attaaaaatt
    66361 aactatatta aatattaata aaaaatatag taaaacttaa taatacatac aataaaactt
    66421 aatataaatt aagtatatga a
//
LOCUS       DAMJWU010000008        63985 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMJWU010000008 DAMJWU010000000
VERSION     DAMJWU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 63985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.008"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(157..2127)
                     /gene="ftsH"
                     /locus_tag="PC460_000700"
     CDS             complement(157..2127)
                     /gene="ftsH"
                     /locus_tag="PC460_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2599600.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2161..3549)
                     /gene="tilS"
                     /locus_tag="PC460_000701"
     CDS             complement(2161..3549)
                     /gene="tilS"
                     /locus_tag="PC460_000701"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF2599601.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3732..4160)
                     /locus_tag="PC460_000702"
     CDS             complement(3732..4160)
                     /locus_tag="PC460_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF2599602.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4177..4983)
                     /locus_tag="PC460_000703"
     CDS             complement(4177..4983)
                     /locus_tag="PC460_000703"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF2599603.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5185..5670)
                     /locus_tag="PC460_000704"
     CDS             complement(5185..5670)
                     /locus_tag="PC460_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2599604.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5721..6380)
                     /gene="spoIIR"
                     /locus_tag="PC460_000705"
     CDS             complement(5721..6380)
                     /gene="spoIIR"
                     /locus_tag="PC460_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2599605.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6525..7205)
                     /locus_tag="PC460_000706"
     CDS             complement(6525..7205)
                     /locus_tag="PC460_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2599606.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7189..8079)
                     /locus_tag="PC460_000707"
     CDS             complement(7189..8079)
                     /locus_tag="PC460_000707"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF2599607.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8197..9747)
                     /locus_tag="PC460_000708"
     CDS             complement(8197..9747)
                     /locus_tag="PC460_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2599608.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9841..10107)
                     /locus_tag="PC460_000709"
     CDS             complement(9841..10107)
                     /locus_tag="PC460_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF2599609.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10255..11115)
                     /gene="yabG"
                     /locus_tag="PC460_000710"
     CDS             complement(10255..11115)
                     /gene="yabG"
                     /locus_tag="PC460_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF2599610.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11199..12137)
                     /locus_tag="PC460_000711"
     CDS             complement(11199..12137)
                     /locus_tag="PC460_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF2599611.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12247..12408)
                     /locus_tag="PC460_000712"
     CDS             complement(12247..12408)
                     /locus_tag="PC460_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599612.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12418..12915)
                     /locus_tag="PC460_000713"
     CDS             complement(12418..12915)
                     /locus_tag="PC460_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2599613.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(13030..13740)
                     /locus_tag="PC460_000714"
     CDS             complement(13030..13740)
                     /locus_tag="PC460_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF2599614.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13916..14446)
                     /locus_tag="PC460_000715"
     CDS             complement(13916..14446)
                     /locus_tag="PC460_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599615.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14463..14693)
                     /locus_tag="PC460_000716"
     CDS             complement(14463..14693)
                     /locus_tag="PC460_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599616.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14708..16102)
                     /locus_tag="PC460_000717"
     CDS             complement(14708..16102)
                     /locus_tag="PC460_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2599617.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16228..17481)
                     /locus_tag="PC460_000718"
     CDS             complement(16228..17481)
                     /locus_tag="PC460_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2599618.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17532..18686)
                     /locus_tag="PC460_000719"
     CDS             complement(17532..18686)
                     /locus_tag="PC460_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2599619.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(19246..19815)
                     /locus_tag="PC460_000720"
     CDS             complement(19246..19815)
                     /locus_tag="PC460_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2599620.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            20018..20245
                     /locus_tag="PC460_000721"
     CDS             20018..20245
                     /locus_tag="PC460_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2599621.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20464..21207)
                     /locus_tag="PC460_000722"
     CDS             complement(20464..21207)
                     /locus_tag="PC460_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2599622.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21375..22247
                     /locus_tag="PC460_000723"
     CDS             21375..22247
                     /locus_tag="PC460_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2599623.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22247..22912
                     /locus_tag="PC460_000724"
     CDS             22247..22912
                     /locus_tag="PC460_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2599624.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22933..23790
                     /locus_tag="PC460_000725"
     CDS             22933..23790
                     /locus_tag="PC460_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2599625.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23946..26492)
                     /locus_tag="PC460_000726"
     CDS             complement(23946..26492)
                     /locus_tag="PC460_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2599626.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26492..27178)
                     /locus_tag="PC460_000727"
     CDS             complement(26492..27178)
                     /locus_tag="PC460_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599627.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27261..28250)
                     /locus_tag="PC460_000728"
     CDS             complement(27261..28250)
                     /locus_tag="PC460_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2599628.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28237..28923)
                     /locus_tag="PC460_000729"
     CDS             complement(28237..28923)
                     /locus_tag="PC460_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2599629.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29423..32629)
                     /gene="carB"
                     /locus_tag="PC460_000730"
     CDS             complement(29423..32629)
                     /gene="carB"
                     /locus_tag="PC460_000730"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2599630.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(32822..33871)
                     /gene="carA"
                     /locus_tag="PC460_000731"
     CDS             complement(32822..33871)
                     /gene="carA"
                     /locus_tag="PC460_000731"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2599631.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(34128..37334)
                     /gene="carB"
                     /locus_tag="PC460_000732"
     CDS             complement(34128..37334)
                     /gene="carB"
                     /locus_tag="PC460_000732"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2599632.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(37387..38430)
                     /gene="carA"
                     /locus_tag="PC460_000733"
     CDS             complement(37387..38430)
                     /gene="carA"
                     /locus_tag="PC460_000733"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2599633.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(38456..39172)
                     /gene="pyrF"
                     /locus_tag="PC460_000734"
     CDS             complement(38456..39172)
                     /gene="pyrF"
                     /locus_tag="PC460_000734"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF2599634.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(39548..40183)
                     /locus_tag="PC460_000735"
     CDS             complement(39548..40183)
                     /locus_tag="PC460_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF2599635.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(40207..41121)
                     /locus_tag="PC460_000736"
     CDS             complement(40207..41121)
                     /locus_tag="PC460_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599636.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            41722..42951
                     /locus_tag="PC460_000737"
     CDS             41722..42951
                     /locus_tag="PC460_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2599637.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWNIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(43156..45537)
                     /locus_tag="PC460_000738"
     CDS             complement(43156..45537)
                     /locus_tag="PC460_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF2599638.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(45509..46234)
                     /locus_tag="PC460_000739"
     CDS             complement(45509..46234)
                     /locus_tag="PC460_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF2599639.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKYVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(46224..46679)
                     /locus_tag="PC460_000740"
     CDS             complement(46224..46679)
                     /locus_tag="PC460_000740"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF2599640.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(46961..48142)
                     /locus_tag="PC460_000741"
     CDS             complement(46961..48142)
                     /locus_tag="PC460_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2599641.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(48132..48824)
                     /locus_tag="PC460_000742"
     CDS             complement(48132..48824)
                     /locus_tag="PC460_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2599642.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(48958..49674)
                     /locus_tag="PC460_000743"
     CDS             complement(48958..49674)
                     /locus_tag="PC460_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2599643.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            49799..50533
                     /locus_tag="PC460_000744"
     CDS             49799..50533
                     /locus_tag="PC460_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2599644.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            complement(50818..51669)
                     /locus_tag="PC460_000745"
     CDS             complement(50818..51669)
                     /locus_tag="PC460_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2599645.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGTL
                     IKSDTIESKSERIKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(51695..52633)
                     /locus_tag="PC460_000746"
     CDS             complement(51695..52633)
                     /locus_tag="PC460_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2599646.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(52736..53386)
                     /locus_tag="PC460_000747"
     CDS             complement(52736..53386)
                     /locus_tag="PC460_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2599647.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKTKEAVEKYREYFADKGIFENKIYENIKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            complement(53536..53724)
                     /locus_tag="PC460_000748"
     CDS             complement(53536..53724)
                     /locus_tag="PC460_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2599648.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(53831..55015)
                     /locus_tag="PC460_000749"
     CDS             complement(53831..55015)
                     /locus_tag="PC460_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2599649.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGLNQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLSVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(55157..56074)
                     /locus_tag="PC460_000750"
     CDS             complement(55157..56074)
                     /locus_tag="PC460_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF2599650.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTETGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(56096..56824)
                     /locus_tag="PC460_000751"
     CDS             complement(56096..56824)
                     /locus_tag="PC460_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2599651.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(57020..57220)
                     /locus_tag="PC460_000752"
     CDS             complement(57020..57220)
                     /locus_tag="PC460_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599652.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(57345..57725)
                     /locus_tag="PC460_000753"
     CDS             complement(57345..57725)
                     /locus_tag="PC460_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2599653.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMRQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(57998..59356)
                     /locus_tag="PC460_000754"
     CDS             complement(57998..59356)
                     /locus_tag="PC460_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2599654.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDGEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGLLRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            59804..60256
                     /locus_tag="PC460_000755"
     CDS             59804..60256
                     /locus_tag="PC460_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599655.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRIFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(60413..61030)
                     /locus_tag="PC460_000756"
     CDS             complement(60413..61030)
                     /locus_tag="PC460_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF2599656.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(61453..62265)
                     /locus_tag="PC460_000757"
     CDS             complement(61453..62265)
                     /locus_tag="PC460_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2599657.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            complement(62341..63759)
                     /locus_tag="PC460_000758"
     CDS             complement(62341..63759)
                     /locus_tag="PC460_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2599658.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
BASE COUNT        20362 a        11609 c         6964 g        25050 t
ORIGIN      
        1 ttttatttaa ataattttaa aataataaaa aaactgaaag tatcaattaa attaatacat
       61 gcaaatataa tttgcttatt aatttaatat tatactttca gttttagtat ttaatccaca
      121 tcagataata atcttaaaat gaccaaggtc ttaaaactat ttatctttat cagatttatc
      181 ttctaaattt ttatttatat caataacatt gtttctttct tcttcatctt tttctaattt
      241 tatagttaat tgttcttcaa cttctttaga agaattttcc tctgtaactg cattttcaat
      301 ttcattcaga ggtaactctt tattaaatgc ttttacaaat tgttctgcat ctaaagtttc
      361 atatataagt aaagcttgtg ctacatactc taatctatcc atattttctt gtaatatact
      421 tcttgtctta tcatatgcac catcaactat acgctttgtt tcttggtcta tctcaaaagc
      481 aacttcttca gaataatttc tcttacttga aaaactatta cctaggaata cttcatcatc
      541 actgtcaaat gacattggac caagtttaga actcattcca tactttgtta ccatacttct
      601 tgcagtagct gtaactcttt ctaagtcatt tgaagcacct gtagatacat ccttaagtac
      661 tagctcttct gctactctac caccaagtaa aactacaata ttttctttca tttcattttt
      721 tgtagcatag aacttatctt caacaggaag ttgcattgta aaccctccag ctcttcctct
      781 tggaactatt gtaacttgat gaacaggact tacttcttca agtacatggg cacaaactgc
      841 atgccctcct tcatggtatg ctgtaagtct tctttctttc tcacttatta ctttcgattt
      901 tttagccaca cctgctataa cctttgttat agcttcttct atagtttcca tcttaatttt
      961 cttttctctt tttctagctg ttaatattgc agcctcattc attaagttct ctatatcagc
     1021 aggagtaaat cctggtgttc ttctagccaa gacatccatt tttacatcat cacttaatgg
     1081 tttatttctt gagtgaactt taaatatagc ctctctacct tttacatctg gtgtaccaac
     1141 aacaacttgt ctgtcaaatc ttcctggtct aagtaaagct gggtcaagta tatctggtct
     1201 atttgtagct gccatgatta ttataccttg atttacccca aatccatcca tttcaactag
     1261 aagttgatta agagtttgct ctctttcatc atgacctcca ccaagacctg cacctctttt
     1321 tctaccaact gcatcaattt catctatgaa tatgatagct ggagcacttt tcttagcctg
     1381 ttcaaataaa tctcttactc ttgaagcacc aaccccaaca aacatctcaa cgaaatcaga
     1441 accacttata ctgaaaaagg gaactcctgc ttctcctgca actgctcttg atagatatgt
     1501 tttaccagtt ccaggaggtc ctaccataag cattcctttt ggtattcttg cacccaattc
     1561 aatatatttc ttaggattct ttaagaaatc tactacctcc tgcaaatctt ctttttcctc
     1621 atctagtcca gctacatctt taaatgtaac tctagttttt tcatcatctt tatgaacctt
     1681 agcttttgat ttgccaaaac tcataacctt tccgcctcca ccttgagact gattcataaa
     1741 cacgaaccaa ataatcacca tgaagaatat taacagtaat gtaggcaaca tttcgacaaa
     1801 ccaaggtgta gatggcttag cttcaccacc aaaagttagt ttcccagcct tagcttggtc
     1861 taacacctca tctgcaagtt tatttcccat cacttctgtt ggaacatatg acttaaactt
     1921 tgtatttgta tcttttatag taccttctac agaagtttga tttacaaagt ataatcttga
     1981 tatattctca tctgttaatt ctctatatac ttcagagaat tttaaatctt ttattttttc
     2041 tgtaggctta cctgagaatt gaactatacc tactataatt ataaaaacaa gtagataaaa
     2101 acctgcccct tttagcaact tattcaatat agaagccctc ctttcataca ttgctttgcc
     2161 ctataatttg ttaaaattaa tttttaatac ttcttttgtg ttttcatcaa ttttataatt
     2221 ttcactgatt ttataatctc caacacatat tattccttca ctatctgtga ttataggaat
     2281 tttacatctt tcttctcttg ggatttttaa atctatgaat aaatctttca cttttttact
     2341 tcccatagca agctttatct tatcaccttg ccttctgctt cttattacta tacccccttt
     2401 taccttatta aaatcaaacc ctttagatga attgtctaat ttcatactct tatacctatc
     2461 tatgctcatt acttgggtct caataatttg ttttaattct tttattttaa taaacccatt
     2521 actaggtaca ttgtaataaa attcaatttc ctcattaaca atttcctcat tcgttaatat
     2581 tatactgtct ttttttctat aaacaaatat atttcttgga agagttaaca ttttatttac
     2641 ttttgaatta tcttctaaag acattatatc ttctatatgt ctttggtcaa caaaattagt
     2701 atctcctagt atatgtttta tggaatttct aagaactcta actttgatag catcatgtaa
     2761 gcaaacaaaa tcgtctaaat ttatctctac aaagcctttt tcttttatat ttgaaacttc
     2821 tctaaattta gcttctgcct ctttctcaat ataatcgtta tcacttttta aactattgct
     2881 catcctcact atagattcta ttacatttga attaaaatta tccttcatat atggtaaaag
     2941 gtctaacctt atcttatttc tagtgtatat attttctaaa tttgttttat ctatcctcgg
     3001 atttaaatta taagcttcac aatactcctc tatctcattt ctttccacgt ctaatatggg
     3061 tctaattatg caattatctc taatataatc aattcccttt aagcctttta aacctgttcc
     3121 tctcatgata cgcatcataa ccgtttcggc ttggtcattg agattatgac ctattgctat
     3181 tttattggct ttaatcttat ccttaatttc ataaaacatt tcatatctta gcttccttgc
     3241 tccttcttct aatgataatc cctcattttc acaatatttt ggtacattaa tagactttac
     3301 aaagaatata attcccatat cctcacaaag tttagataca tataatgcat ctttttgagc
     3361 ttctatccct cttatttgat ggtttaaatg tgctgcatat atttctatat taaaatcttt
     3421 ttttaatcta tttagtacat gcaataaaca aactgaatca ggtcctccag aaagacctaa
     3481 aactatttta tcgccttttt gtattaaatt atgtttattt atagtactta gtaccttatc
     3541 aaatatcata tttatccctc ttacacttag ttttattcac ttttaagtat actttctata
     3601 aaaaaacgct aaacccttgt atcaatttta agaaattaaa gtttaatata aatataataa
     3661 taccaaataa attaaataaa aggtagtttt ttcactacct ttataaaatt tctggaaaat
     3721 agccatagtt attatttagt acctatgtat atatctattt tattcctacc taaaaataaa
     3781 tcttgtacct ttatattata gtctattttc aattttatgt gttccccttc ttgtatattt
     3841 atatctaatt ctcttgtatc aatatcaatt aataaagtac cttgtggggt cacatacttt
     3901 gtagtactac tttctccaca tttaaacagc attgttgagt taatagcacc aaactttttg
     3961 atactaactt gatttttaag tatctttata gtattagtta tttctatatc ttcttctttt
     4021 tccttgtaaa taacatatat tccatcattt ttataaaaaa ttttaccaaa ggcagtcatc
     4081 tctatggtgt ccatatttcc tttttcatca tattgtctag tatttatact tatttttaca
     4141 ctcaacgatt tttcctccat agattcttca tataaactag tatttttgta tttctacttt
     4201 ttgaatctta tcaatttttt tctttaagaa ctctcttgct atatctgaaa atttttcagg
     4261 tatatctgat acataatatt gataagttcc atgaatttct gtattgttta taatattttg
     4321 cacctctaaa atctttttta aatcctttgc agtctcttta gctggattaa caagttttat
     4381 atgctctcca acttcttcac ctattgttct ctttaaaata ggataatgag tacatcctaa
     4441 cactaaagaa tctatatttt tttctttcaa ttcttgtaaa tatatctttg ctgtcagttt
     4501 agccacttct gtatttgccc atccttcttc aacaataggc acaaataaag gacatgcttt
     4561 attaacaatt tctatactct catctatttt agatatttcc aagttgtatg cttttgaact
     4621 tatagttcct tcagttccta ttattcctac aattttgttt tttgtagaac ttacagctgt
     4681 tctagctcct gcttctataa ctccaattat cggtatatca tatttttctt gagcttcttt
     4741 taagcttctt gcagtagcag tgttacatgc tataactata gcttttacac cttttgtttt
     4801 taaaaagttt atagcttgaa aagtatattt aattatagtt tctttggatc ttgaaccata
     4861 tggtattctg gcagtatctc caaagtatat tatatcctca tttggtaata tcttcattat
     4921 ctcttttaga actgttaatc cacctaatcc cgaatcaaaa actcctattg gtttattgtt
     4981 catctttata caacacctac tttacatttt tgtgtttaac aacaacttat ttatcgctaa
     5041 aatttatatt tatattcata attaatatat tattacctaa atgattatta tagtttattt
     5101 tacacaaaaa ataaaggagc tatcttaaat tagaaaataa tttctacaaa aagacaactc
     5161 ctttcactac ttaaaaattt aaatttattt tgcaaacaca tgatttccta ttctcatata
     5221 aggattcaat ctatttatcc atttgcaagt ggctatttct ggattccaaa aatatactgc
     5281 tttattagtt gggtcagttc cataaagagc ttcctgagct gctttacgtg ctgagtctgt
     5341 tggtgccatc tttattgaac catcaagcac aactgagaaa gcattttttt gatatatgac
     5401 ttcttctatt gtgtctggaa atctagggtc caatactcta tttattacta cagctgctac
     5461 agcaacctgt ccctcatagc tttctcctct agcttctcct gcaaccaact tagataataa
     5521 catcaagtca ttatcagata aatttattac ttctttcttt gctatatttt tttcttgttt
     5581 gtttactttc ttaatatttt ctgttggttc atatgcaaat acctctttaa taggcataaa
     5641 tataaaagtc aaagctaata gtgtagccat cagtttactc cacctgtcca tgattaaaac
     5701 cactccttac attcttattt ttaaaatttt ttctctaaat ccttgactac ttcaagtact
     5761 ttaaatttaa tttttactct acttgttttt tttggtgtat cttgttttat taacttatat
     5821 tctttatcag ttaaaacttc cttcaactta tcatctgtag atttatctat cactccatta
     5881 gattcatcaa caaaacatag tggagggaac ataacacacc accagttttt tccttcacct
     5941 ttcccaatga taatctttaa tgctttatat tcaccagctg gaagaactat attagaatat
     6001 tgtttagtag ggaaattgct atattgtata cctaccttta cactgtaatt atatccattt
     6061 ttaagaataa tatttttact tatttcttcc aaactactat attccttttt tataatagct
     6121 tcactttctt ttatactttt tgaattttga agctttggct gtaaatattt tattacttca
     6181 tctctaactt ttagtttaag ttcttggtct tcatctgtat tgctgtttgc tataacatga
     6241 aatcttatta atttatcttt ataatctttt gatatattgt ctactttttt tacctcacca
     6301 tttattacta ttgtcattat tgatatgact gatataagac ttaatattaa tagtcccaat
     6361 ctaatcttaa tttttctcat tgaaaattcc ccctcaagat aaatttatat catcaattat
     6421 gtccaattaa atttaattta ttcataatat tttttaatac taataaaaaa agctatctta
     6481 aaatgattac aatcatttta aaatagcttt ttaaacctta tattttataa gtctttacta
     6541 atcatagcct tatctatcat aaactgctct gctttttcta tatgctctgt agcatacttc
     6601 ttggatagct cactatcacc tttaattata gcatcaagta aatatttatg ctcttcaaca
     6661 atattatgtg atgtatcata agcagacata taggtaactc taaatctata tacttgttcc
     6721 cacaatgagt ttattaattg gattaatttt ttattattag ttgctttaaa tatacattca
     6781 tgaaattcta catcaagttt taataacgca tctacatcgg attccaaagt gcttttttta
     6841 aattcttctg atatatcttt aagcttttta atatcttcat cactaattct ttcagcggca
     6901 agatatgctg ctaagccttc taggcttgct cttacttcta atacatccat aatatccttt
     6961 aatgacatat ttgccacata cgcacctttt ctaggcaaca taacgactaa accttctaat
     7021 tcaagctttc ttattgcctc tctcactgga gtcctactta ctcctaattg ttctgctagt
     7081 tgcacttcca ttagcctttg accaggcttt aattttcctt ctaagattgc ttctcttaaa
     7141 ttctcaaaga ctacatctct taatggcttg taattatcca agttcagttt agttaaattc
     7201 tccacaaatt tcaacccctt tttgactgct attaacaaca tacacctgtt tatatttttt
     7261 taataattct ttttttccaa ttagagcatc ttctttattt ttaaatagtc caaaaactgt
     7321 aggaccactt ccactcatca tagaacctaa agcattattt ttcatcatta cttgttttat
     7381 atcacttatt tctttgtgtt tacctatagt aacattttca agaatattta ccatgctttc
     7441 tgatacagct tttatatctt cagattttaa acactctatc aaatatttat tatttggtct
     7501 ttttttaata ttattcaaat ctaatccttg ataaacttct ttagtagaaa cgaataagtc
     7561 tggtttgcat atcaatatat ttgtatcaca tggtaaacct ttgatgtttg ttagcttttc
     7621 tccaatacct tgagctaagg caggtcttcc tgatatacaa aaaggaacat ctgcgcccaa
     7681 attaagtcct atttctttta attcatcttc agataatcta agttcccata ggtgatttaa
     7741 acctactaat actgcggctg cattagaact tcctccagcc atccctgctg ctacaggaat
     7801 atttttttct atgaatattt ctacaccttt ttttatataa aatttatttt taagtatttt
     7861 ggcagcttta taaacaatat tatcttcatc taaaggaata tctaaactag tgctttttac
     7921 ctttatttca tcttcgtcta attcttttat ctttactata tcgtataaat ctatcgtttg
     7981 cattatcatt tcaacaaaat gataaccgtc ttgccttttt cccaagacat caatggataa
     8041 atttattttt gctctactct ttagtcttat aaaatccatt ctatcctcct tgtttaaaga
     8101 ataatgcatc actaacaata atattatact acaaaaaata aaataatagg gtattataat
     8161 accctattat acttaattac tttaaatttt ttaatattaa tctactaata cgactttttt
     8221 ttctaaaata agacacttac caggcttaat tggttcatca agttttatat cattaagttc
     8281 tgcaatttca ttttctgttg tattgtattt ttttgctata ttccagaaag tatctccttc
     8341 tttgcatata tatacgatta tgctaggagc tttagataaa tcatatacac cttggtcttc
     8401 tcccttgata ataaaatttt ctgctttctt atctaatgct tcagtaaatc tcttaatttt
     8461 gataatcaag tctatctggt ccctattcaa atcaacttcg actttatcaa tacatgctgt
     8521 attaaacaca gatgctgtat cagttagatt atccatttct atatcatgtt caaatggaat
     8581 ttcctcacta attttataaa ccaattttaa accttcaact gggacaaata aaatttcaac
     8641 ttttatgatt ccttgtataa tgcttttatt tccttcaata taactatttt ctatagacat
     8701 ggttggacaa acgcttacaa tatcttttat ttgtatatca tcattatcat ttcttatgcc
     8761 ttctctaacc ataaatgttt cttcactacg tcttaaagtc ttatttaatt gaataggttt
     8821 gtggtcaaat tttattatct tttgaggcga atatgcatct tgtaatactt ctcttgtaac
     8881 ttcatctgta acttttacct tacaacaagc catacaatct atttctaaaa gacctgtgtt
     8941 gctttcacta ttttgcttga aaatatgatt aaaatctgac atagacaaca atacttcttc
     9001 agtcatacca tcactaactc cagggacttc tacaaattga gtaaactcaa tacctactct
     9061 atccaactct acaagttcac cctcatatgt acaagctaac ggatttattt ctaatactcc
     9121 accaataata actttattgt cagtcactct actttctttt actttaacac atggatttaa
     9181 acttattata gattgaattt cctctgtgtt tattgttata gtatctctta tagaactttc
     9241 tgctttttct attccaacta tatcttgaaa acaaatttcc tttctgtgct tttgtatgcc
     9301 ttccacttgc gccacatctt ttacaatgtc taacctttgc ttttcaaaca aacttcccct
     9361 tatattcata agagctccta ctttaatctt tctctcattc attatagtac aatccatatg
     9421 ctctacttct gaaaataaca tgtattccat atcttgaaca acattgtctt tttctattac
     9481 ttcgtttata tctacttttc cgtctacatt agaaatagta tttttatcat ctgcaatata
     9541 gattacatta taattaaaac tacctctgca aagtatcttc ccatctgcca tttcaatttt
     9601 tttaagtgat atgtatcctt ctgtttttac aatttcatat acatctaatt ttttgtctgg
     9661 tacaactgcc tccgcttcaa tgaatgtttg aaattttcca aagtctattc tattgtcaac
     9721 tttaattaca tctttaatta attccatagt ctacctcctt aagtattata aaaatatcac
     9781 actatattta tatttaagga gcaaaaaaaa aattacacct atttttaaat aggtataatt
     9841 ttaacttatt tgtaatttat cttgatctct aaaaacttgc aactttacat tagatgttaa
     9901 taaatctgag taactataag atactctagg atatccatta ctttcatcat ctaactttat
     9961 aacaaacacg cttggataaa ctttttctag tataccctct tttgtaataa tctgctttct
    10021 tcctttatta gctttcaaca atattttctt tcctatatgt ctctctaagc ttactcttat
    10081 cttatctaga gtttgaacag tagccacaat atcaccttct ttaacgttta tatgtttaca
    10141 taatatcaca tataaacatt tttgtcaaat gtttaaacta attattttat atattttttg
    10201 caaaaatgtc aatactattt ttgaagatat acagtataat tatgttaatt tttgctaatg
    10261 taatattgtt tttggcattg cccatctaaa ttttcctctg gtttcagaac caccaattcc
    10321 cttaacacct gtaatagtct gctctataac ctcatcaata ggaactacta catcaattct
    10381 tgaacaagca attttctcca ctataaaaac agggtctaat gcatgtatca ttatccttcc
    10441 aggcgaactt gcatagtttg ctcctgcact tattattttt tcataatttg attggcatgc
    10501 tcctgcgaat atgacaagat tatccaaatt tggttgccat ttacgtgctt cttttactgt
    10561 tttaacaaaa tttgaagagt ttctatagtt actcatatct tctatatctc ctctttttat
    10621 tgtcattgca tcatgacctg ttattactag tatatccgga ttatattttt ctagtagtgc
    10681 tcttacttct ttatactgat tttgctctga aacagctaca cctactgcag gtattccaag
    10741 ttgagcatat acatccaagc aaatttttaa atattcttta tctccatcta tctgtagtac
    10801 ttttccaggc attccatatg tgttaggatt taattgaagt tttggaactg ctctagtcat
    10861 tcttttttgt ctttctttag cttttcttac tgacttatat aataaatttt ctacattttt
    10921 atctattaat atatctttta tatcaggtgc ttttactaat tctaggtcat caatataagc
    10981 atctgctatt actctaaatg caattccctt tagtatagct attttctcat tattttcatc
    11041 tacaccaaaa gagactattt taaatactat atctttgtta tgtgactttc tagctacaat
    11101 gtctcctacc ttcatagtta aacctccaat aaatttttat cttagtttat actatgttcg
    11161 atataaaact ttgtttccta atgtcaaaaa aacatttctt ataatacttc ttttatcact
    11221 cttaaagcat ttttatagta tatcttttct atatcatctt cactaaaccc ttcttttttt
    11281 agaggttcat acagtttacc catctgagat atatcttcaa tctcaacttt tgaatctatt
    11341 ccatcaaaat ctgaccctag agatacaaca tctattcctc caacatttac aatatgtttt
    11401 atgtgtctaa ccatatcttc aagtttactt tcacttttat cacttaaaaa taagtggaag
    11461 aagtttatac ctgtaactcc acctttattt gctaatactt taatcatatc atcagttaaa
    11521 tttctagaat gattcatcat tgctcttgaa ttagaatgtg tagcaattat tggtttagaa
    11581 gaaatctttg caatttcata aaatccacca tctgaaatat gagaaacatc aaccaacatt
    11641 cccaattcat tcatcttatg taccacttct cttccaaaat cagtaagccc tttttctcta
    11701 tattctactt tattatgagg aaagctcaat tcattcacat gattccaact tattgtcatc
    11761 attctaaccc ctaagtcgta aaatttcttt aaattttcta atttaccttc aagtactgca
    11821 ccttcctcaa tagataaaag agctgtaagt ttaccttctg attggttttt cataatttct
    11881 tcatagttag tagctaaagc tatttcatca ctattttttt tcatttcctc atgaaattta
    11941 tttgccatac tcatgcaata atcaaaagga tgttttactt cttcagtatc aacaaaaaga
    12001 gcaaatgttt gagctaatga atcccccttt ttcaatctgt ctatatcaac agaatattta
    12061 ttagacttaa gttcacttgt ttctacattt tccattaatt ttgctatagt atcacaatgt
    12121 aaatcaataa atttcatatt tataccccct gtatatttta tatttatatc taaaaataga
    12181 aatagttgtc cttatagaca actattgaca actatttttt aatattcttt ttaatattac
    12241 ttaatcttat tcttctttta agttaggtat tactttttta acttcttcaa tttgatgcca
    12301 tgttttatct atattatttt cactaatata tttttttaaa tcagttaaag atgtaaaatc
    12361 tttaactgat aaattgtcat catcatctgc aaatcttaaa ataaacatga gactccctta
    12421 ctttccacaa catttcttat atttttttcc gcttccacat gggcatgaat catttcttcc
    12481 tatttttgct tctttaacta ttgttttaga cttcttataa gcctttatta tttcttttct
    12541 ttggtctgct ggtagtatat cttcccaacc ttgtaaatta tatagccaat gagcctcaac
    12601 agttaccata ttgttatata atttttccca atctattttt atagaaactt ctttatcagc
    12661 ttctacttct tctaaaacta tatcttcttt taagctttca cttattccat ctatgaatcc
    12721 catgaaaaat tcattagtag ttccaaattt ttcagccaat tcacttactt taccttcaac
    12781 aacatccatt ttattattta atatttcatt gtatattcca gcttcaactt tcaaatattc
    12841 ttcccagaat tttacttctg cttcttgact ttcatgatta tcacttaaat ttctccattc
    12901 agtatataaa ctcatacttt ttcctcctaa gtatacctat atttttatag tttttatagt
    12961 ataaaatctt ttagttagat tggaagtttc ttctttttga ttataccata ttctatttaa
    13021 agttaactct tatattatat cataattaaa caattctttt aatactttta tagcatactc
    13081 ttggtttata aaataagggc ttgtcgcaat tgttattact ttggcttttt taattaagtt
    13141 ttttatcttg taaaatttga gttcaaatgg tatatagtcc atatcacttg aaaatatatc
    13201 caaatctatg tcaagaacaa actctgattc tacttctaaa tcaaatccat atagactatc
    13261 tattattatt aattctgaaa atatgttata ttttaaagca ggttttataa aacttcccac
    13321 atttaaaact tcatttgtat atctaaatac atcttttaga ttatttacat ctacatcata
    13381 gttctcaggt tctctagtat ccttatgttg gtctacatgc actaacttgc atccttttgt
    13441 aaactcaccc tttaacaaag actttgccca aaaataaaat gcatggttat gattgtcaaa
    13501 cacatatatc tttttatttc ccaggacata ttcgaccata ttctccaatc cttttgcttt
    13561 tatttcctta ttttcatcta cttcattaaa tactacctca ttgcctaacc ttacatcatt
    13621 taatgtacca tttataagtt ttggaacgta tatgctctta ttttctcttt tatcatatga
    13681 aaatacatta tttcctactg gtttttctat ataaaatcca ctatattcat aattttccat
    13741 aaaactttat ttcctccatt gttatataaa tatataagtt ataattttaa aaattaaaat
    13801 gcacttattg taaatcatat catctattat agaaaacatc ttatataact aaatataata
    13861 tcttctctca atatgtatta tacaataagt gtaaaatata tttatataaa atatactaca
    13921 ttcctataac aaaaccatca gcttcaattg gaagataact aactcttcta ttcaactcca
    13981 cctttccaaa aaatactacc caatatggtc taaaaatact ttcaatttta actatttcaa
    14041 tctctgggat tcctcccata aacctatgag ttaatctcat agccatttta tttgcctttg
    14101 cttttactct tcgttcgtcc ttatcactat actgcacaat attacaatcc acatttttta
    14161 caacttcata tgtaggaaaa tcttgtacta tactaccaga acctgtagaa ccattcacta
    14221 aaatttttaa ttcatttata tcattagaat atctcctatt cttgaaaatg gatttcgttt
    14281 tgtgaatttt aagtgtgata attttaaact ctatataatg aagtctaact tcatgagcat
    14341 atttgttctt taatattagc ttactaaata aagaattttt tccactttta gctatctcta
    14401 tagcttcttc ttttgtaatt cttataggta acgctcttat atccataaaa tcattctctt
    14461 tcttattttt ttcttgtcat ttttaaaact ctatatatat aatcaaaaaa tattacaatt
    14521 acaactgaca taactccata tggaattatt tttccaacta taatatcatt tctagtcaat
    14581 ataccataaa atataaatcc tgttattaaa aagtacaata atactagaaa accgtctatt
    14641 ccaagccatt ttatttctaa atcttttttt accttagaca ctaatttaga catagcagtt
    14701 tctcctttta tagattattt tgtgtattta aattttcacc tattctatta ccaatcatcc
    14761 atgcaataaa tgaaaaaggt atagctatca caagaggctc taactcaaag ctctgaactg
    14821 gaaatatttt aaaataacca aaagtcactc caccaacaat cattcctaaa ataccaccca
    14881 tagatgttct tcttccattt ccaagaagcc cacctactat aggtacaaat gccgaaccca
    14941 tccaaatact tcctacaaat atacatgcat catatacatt ttcaaatcta aatgctacgc
    15001 taagccccat aactgctgct aaacatacac ctattctagt aaactttact aattgtttct
    15061 gagataactc tttttttgta attcttccat atatatcatt tgctaatgta gccccagctg
    15121 ctagataata gctatccaat gttgatatta tagaacctaa aattcctatt ataaatatta
    15181 ctcttattcc ttgtggtaat gtatctaaaa ctaaagttat atatccagca gctggagcca
    15241 tttctggata agtagctctt actataagtc ctgtcatagt taacaccatg tcaaaacata
    15301 tccatagtga aaaacaggtt aataaggctc ttcttccaac cttaggagaa tctgctgctg
    15361 aaaatctttg ataaaaagtt gggtctgaat aagctccaaa agaatataca attaacatca
    15421 atgctttcat agctgacata ccctttgtag ggtgcataag ctgtggtgca tctttgagtg
    15481 cttctgtaag accttgaaac cctcctattt gttcaaattg acctggcata accatagtta
    15541 aaccaaatac catacaaaaa aacaatacca tatctgtaat ggcaactccc ataagcccac
    15601 caagtactgt aagtacaacg actactccta ccattattaa tcctattaat tctttatcta
    15661 ttttccatgc tacgttacct atagttccca tagcagttat ttctccaagt tgtgcacaat
    15721 atataaacat caaaattgcc ccaattatag caacatgctt gccataaaaa ctttccagta
    15781 aatcaggaac tgttatattg gttcttatat gaatccttgg accaacccac aatgctaaag
    15841 gcattctccc aatatgtgca gctattgacc aaactgccca tgcagatatt ccatatatag
    15901 ccgcatactc tacactacct actgtaccaa ctcctccata ccatgatgca accaaagttc
    15961 ctactattaa ggaccatggt aaacttcgat ttcctaggta ataactttcc atatctttta
    16021 catttctaga aaaataataa cctattccta ataatataaa tgcaaatata actataatcc
    16081 cattttccca aaatgttaac atatctaatc tccacattct tatattttaa tttttaactt
    16141 taatatctaa atatgaacaa ctctacccat ttccataaaa ataggtagag ttatataata
    16201 attataagta tttaagtaat cttgttatta agcaatttta ttgtttaaac ctttattttc
    16261 tcttttattt attttattta aagcataagt agcaaatatc cctataactg cacatactac
    16321 agataaaata aatatatatg tatatcctgt ttgtccatat ttatctaacc aattacctgc
    16381 aatagtgtaa taaaacgcat ctggagaaaa tcctataaat gaagcaaatc caactactgc
    16441 tcctgtagtt tctaaagata tattagactc tgaaacagat gcaaagtata tacctctaaa
    16501 tccaaataaa attacactta gtattatcat gtttattaca gctatccata taaatcctgt
    16561 actcttagga ataaccaagt aagcagctgt acttactgtc attaatataa atcctacaaa
    16621 cattactctt atagatgaac caactttatc tgttataaca ccagcaactg gagatgctcc
    16681 catttttata acataagttc ttattattga taaagcagaa actagagtca ttggcattac
    16741 ataaacttct gataagtatg gacttaaata agttaaactt gagaatacta aataagttga
    16801 aaatattata agaccaatta gccaagcctt tggcattgta actactttta acaatgactt
    16861 aatacttaat ccttcatctg atgctccact atcttttttc tcttccacta agaaaaatag
    16921 aagtatacca catattatag atgctgcaga atataaccat actacatatt tgaatcctac
    16981 tgttatttca gcaaatttac caaagaagta aagacctata aaagaaatta atgtacctga
    17041 tacacctgat aacccttcat aaaaaccaaa tattcttcct tgctctgact cacttccttg
    17101 cattctaaca accttaacag aggctgagaa gaaagttaat attgttgtaa atccccataa
    17161 tacaaatatt aaaagtatca tactatatga tgggaatgta gagaaataaa atccaagtat
    17221 tcctgaagca attaaagaaa aagttattaa ttttctaact gagaatctat ctgcaaacca
    17281 tcctcctata aaataagata ccattcctac taatccatat aaactcaata agttccctat
    17341 ttgtgtatga cttaatgcta aagcttgttg cattgggtca taaaatgatg atttcatata
    17401 tggtaaacta tacatagctg ttgttccaaa tgccaatatg aatattatca aaaatctttt
    17461 aacattattt ttgctttcca ttgattctct ctccttaaaa ttttattgtt tataatctca
    17521 ttcatataat tttattttaa tttttctatt gcttgtttaa aatcatttat tatatcattt
    17581 gaagattcta atcctacagc tattcttatt agattttccc cattcatttg aactaatgta
    17641 tgctcttccc ctaagcttgt tgctattgtt gcaagctcta aagaattcat taatcttcta
    17701 ctagcttggt ctcttgtcaa tccattgata ccatccttaa gtttaaatga aactacacct
    17761 ccacctaaac cattcatttg agttttacaa agctcatatt gaggatgaga ttttaatgct
    17821 ggatacttta ccaattccac attctcttct tcctctaaaa attctgctac tgctaatcca
    17881 ttttcacaat gctttttcat cctcatatct aacgttttca taccccttaa aatcatccat
    17941 gcactcattg gtggtaaaga tgctcctgta aaacaacata aacttggcca tctaactaaa
    18001 tctattaatt cttttgaacc tgctactgct ccccctaaag tatccccatg tccatttata
    18061 aacttagtaa gactatgtat tactaaatct gcacctaaat ctattggtct ttgtacaatt
    18121 ggtgtcccaa aagtactatc aactatagct aagcttttat tttcatgagc aatttctgct
    18181 attgttttta tatctactaa gtctaatgat ggatttaaag gactttctat ataaactatt
    18241 ttagtatttg gtctcattgc ttcttttaca ttttgtggat ttactgcatc tacaaatgta
    18301 acttcaactc caaatttaga taacaactca gacatgaata atttagtgtg tgaaaatatt
    18361 ccatctgaac taactatatg gtcaccactt tttaccaatg ctaatatagt agatgttatt
    18421 gcccccatcc ctgatgtact agatatggca ctttctgcat tttcaagcat agccaactta
    18481 gcttctaact ctgtaagagt aggattacac tctcttgtat acacatatcc ccatgtcata
    18541 cacaactctt caaaatgctc tactgtcttt gcagcaaatg ttgctgtttg acatattggt
    18601 gtagccaatg cacctgttgt tgggtctgca ctatgtcccc aatgtattat ttttgtctct
    18661 aaatcttgat taatattgtt actcattttg atttcctccc attttgactt atttatacaa
    18721 cgttttccct accaaatttc atcaatttag cttagtctta atacattttt tcaagtatac
    18781 ttttttattt gttataattt ctaaaaatat gacacttatt ttgttttttt acagttctaa
    18841 ttataaactc tatagtaact atagagtcaa tatgtttttg atattttttt tacaatgttt
    18901 aaaatagtca gtacaaaaat aggtatatct tagaataact aaagtcatcc taagatatac
    18961 ctatttttat atcacaatat aaataattac atataagcat tagttttttt atcataaata
    19021 gatttttcaa gatttatcat cttttcataa atatattata gttaaatatt ttaatattac
    19081 ctaacatcta caataaaata tactcttaaa ataaaattat ataattaaaa atactctata
    19141 aaagctaaaa agtttttcta ttcgctacat ctaaaaataa ttagatagag gtataaatgt
    19201 atttagttta tttatacctc tagtctttta taaattatat ctctactaaa ttgatttttt
    19261 agcacgaata agtgtgctct tggagatacc agtcatcttc acaacctctc tatatggctt
    19321 agaatttaat aaccccaatg catattgtat ctgttgattg ctgtatttct ttggcctacc
    19381 atctttaaag tcctttttct ttttagcaaa ttcttttcct aattgagtac gttctattat
    19441 catatttctc tcaagttctg caactgcaag taatgtagtc aaaaagaatt tacccattga
    19501 agtattttct aaaaggccta tatttaaaat atgaacactt acttctttag caaataaacc
    19561 ttctattagt tgtattcctt ctgatgtact tctagctagt ctatccagct tagttgcaac
    19621 aagtaagtct ccctttttta gagatgatat aattttttca aatttaggcc tgtttgtagt
    19681 agtgcctgta taagtttcct ttattatttt agcatcatta tatttattca aaatttcttg
    19741 ctgttgctga tacaaactgt tgttgtctgt ttgatttttt gtacttacac ggcaatatcc
    19801 atatataata atcatcactt cctaacataa agattttgta cagaagttac ccagaagctt
    19861 gtttttactt atctttatcc attttactaa aacgattttt gggtgtcaat actgttaact
    19921 tttgacacca taataattcc tttataattt gtttgactat gtcaaaaatt accaatataa
    19981 tgaaattaat gagtttatac aaattggagg aaataatatg aatagtctgt taaaagaagc
    20041 aatagatgaa ttaaaaaaca tccattgcgg ggaagttttt ttattaaaag aattgttcaa
    20101 aggctatgaa tggaacaggt tggagatgaa tatcagattg aaattaggaa aacttttttt
    20161 gtatgaagtg gtaactaatc ccaatttaaa tgtagaggtc atcaataaaa attctgctta
    20221 tcagcaaatg tataaaaaaa aatagtgact taaatgtata taatagtata atcttcttat
    20281 tgattcaaaa gttaaaactt ttaatacaaa acaatcattc tattttaaca tttatcaata
    20341 tgtctaaatt tatataatta acacatacga atagtattgt ttaaatcaca ttatttaaga
    20401 tagataaatc ttaaccctac tccagcataa atactaaaat agggcttaga attatctata
    20461 tttttatccc ataaaatgct tagctcttcc tcttgattga acatatatat ctggggaaat
    20521 acaatagaag cgtaaattta taaaatttcc aaagtcagaa aacttcagtt caaacttagg
    20581 tataccaaaa ctttcaggtg ctatttcgtc aacgccaaaa tagatgataa ttccattatc
    20641 tgtaagataa aatgcttggt cttcaggtat aactaactca acattagaat aatacatttc
    20701 tctattttca tttatttgag atttaatgta atcagtaatt atattaatat aatcaactcc
    20761 atctctaaaa atgtcattaa gtaaaagttc ttttccagtt gtcaaatcta tattgtaatt
    20821 attcaatgta ttgtatttag gcccatagtt attagcaaac cccataaggt ttaatatcat
    20881 actcagtaca taatttttat taaaagtaac tgcgtaactg gtagataccc aatagctttg
    20941 ttttggaagt gaattttgag aagttgcgtt attatattct cgttcttctt cctgtaagcc
    21001 atttttaaaa ttatttacag tcattcttac agcattgttt atgttattta aaataactgg
    21061 gtttacatac gtggattgat ttctaatatt gtatataaca ggatacttta catcatatga
    21121 aaaatactta cttctttcaa aatcttcatt aaattgtaca tataatatat tatatgtaca
    21181 ggtatttgta gattgataat tttccattaa aatcctcccc cttttataaa aaaatcagat
    21241 acaatataat atgcttaaaa cagtaataat gttaatggta aatgtaaaga atagaaagta
    21301 tctaaaacag caaaaaatga ctaattttgt ggtataatac ataggattga gagttatttt
    21361 ataggaggaa aaatatgtta ttattagcag ataaatggaa agattatgaa cttatagata
    21421 tgggaaatgg agaaaaatta gaacgttggg gaagttatgt acttagaaga ccagacccac
    21481 aggttgtatg gccaatggaa tctgaatggg ctttatggaa aaaccctcat ggacattatc
    21541 acagaagcaa taaaggtgga ggtcaatggg aacacaaaaa aagatatcca gaacaatgga
    21601 ctataaatta taaaaactta aagtttcaca ttaaaccaac tggctttaaa catactggat
    21661 tattcccaga gcaagcagct aactgggatt ggatgataga gaaaataaag aaagctaaca
    21721 gacctataaa agtgttgaat ctttttgcat atacaggtgg agctactgta gcatgtgcat
    21781 ctgctggtgc agaagtatgt catgttgatg ctgcaaaagg tatggttaat tgggcaaaag
    21841 aaaatataga attatcaggt cttaaaaatc aaacagtaag atttatagtg gatgatgttg
    21901 taaagtttgt tgaaagagaa ataagaagag gtaaaaaata tgatgcaata ataatggacc
    21961 ctccttctta tggaagaggt cctaaaggag aagtatggca aatagaagaa aagttataca
    22021 gttttgtaga tttatgtatg ggagttttat cagataaccc tttatttttc ttaattaatt
    22081 cttatacaac aggattttct ccaatagttc ttgaaaatgt attgtctaca acaataggta
    22141 aaaagacaaa tacaggagat atatacggtg gggaattagg aataccagct tccagagatg
    22201 gtaagatact tccttgtggt atatttggaa gatgggagaa aaaataatga taaaagtcat
    22261 atatgaagat aaccatttat tagttgtaga aaaaccagtt aacatattgt ctcaaggaga
    22321 taatactaat gataaagata tggtaaatct tttaaaagac tatattaaaa aaaagtataa
    22381 caaacctgga aatgtatata ttggtttaat acatagattg gatagaccag taggtggagt
    22441 tatggtgttt gcaaaaacat caaaagcagc atctagattg tctgagcagg taagaagtaa
    22501 gactttcaaa aaaacttatc tagctgttgt tcatggcaag ataaaatcta actcagatat
    22561 actaaaagat tttttatata aaaataaaaa aactaatatg gtaactgtag tgaaaaaaag
    22621 ccataaagat gccaaagaag ctgaacttaa ctataatgta ttagatttta aagaaaatct
    22681 aagtttagtt caagttaatt taaagactgg tcgttctcat caaataagag ttcaatttgc
    22741 aagcagaaaa catccattat acggagacca aagatatggc aaagaaataa ataagattgg
    22801 acaacaaata tctctttggt ccaataagat agaaatttgc catcctacta caaaagaaaa
    22861 aatggaattt acatgtcatc cacctgacaa atatccatgg aatatatttt aaatctcaaa
    22921 tgaggtgatt atatgagtaa aaaattttca ataggagaaa tgtctaaact tcataaaata
    22981 agcatacaga cactaaggta ctatgaccaa atagatttat ttaaacctgt tcatgttgac
    23041 caagaaagca attacagata ttactcaatt gaccaatttt cacaattaga tattataaaa
    23101 tatttaaaat atttaggtat gcctttaaaa gaaattaagt caaagcttga tgaaaataat
    23161 gaagaaattc taaaattact aacaaataaa attaatatag tggatgaaaa aattaaagaa
    23221 ttagagctta taaagaaggt actaagcaca aagaaagaaa ctatggaaaa aggtatgaaa
    23281 gtagatgaag ttggtaaaat agtgagaaaa catattccac gaagaagtat cttatctata
    23341 gagtatgaag aaggattaag tagtgaagaa actatggaac tttccagaag acaaatagca
    23401 aatatattag aagataatat atctgtgttc tatggtggag taagtggact tgtatctata
    23461 gataaatttt taaaagaaga taaagttatt tatgacaata gttttgttac tgttgaaaga
    23521 gacttatttg atataaaatc acaaaaacat ataacagaaa tttcatctgg tgattttatt
    23581 tgtatgacat ataaagggcc ttacaaagaa aattataccg cttttaagaa actggttgat
    23641 tatgttgaat ataaaagaat acctattgaa aattatatat atgaaatacc tataatagac
    23701 cctctgtcta caaacaacga agaggaactt ctaacagaaa ttcaaataca agttaaaaag
    23761 agagaaattg acttaagatg tctagtgtag taatctagtt tacttacaca tcttaatata
    23821 gttgtctatt atcaacatat gatttttcta gatagtgatt cctaacagtg agattttcaa
    23881 aaaataggag gaaatatggc taatgctaat tccctcctat ttttaattaa gccatatcat
    23941 tttaattaag ccatgtcatc acgaagtgca tcaataatat tttctttttt tatcttatta
    24001 atagtataca acattgtaat aaatactata aataaaacac taaatatact aattccaata
    24061 ctaaaccatg gaatcacaaa atcaatatca tctgtaacca ttactttatg aattagccat
    24121 gaagaaatta ctgctatagg aagtccaaag aacaatgctc tcatgccata aaaggcacac
    24181 tcaaaattca tcatcttttg gaaatcatgc tcagacattc ctacagagcg aagcatggca
    24241 agttctctac gacgtaactt aatattagtt gaaattgtat taaacacatt ggcaatcgaa
    24301 atcagagaaa tcataattat gaaagtataa gcaaaaacat tggcaataaa aatgtaatta
    24361 cggttctcat ccatcattcc atacatattg tacagattgt actcattcgt aactccctca
    24421 ctctcaataa tttgctccat ttctgcaact gactgagtag gatttttcga ttgaaagctt
    24481 aaactttcaa ttcctgtaga aacaccagga atctcaaact tatctttggc tgaataagga
    24541 gccaatactc tgaagcttgc tttcttcaca tctgaacttc tgattcctgg aggagaatca
    24601 ggtggcacta ctttgacaaa tgaaatattc acattttgtc tctcaggtat ctccaacttg
    24661 ccattagttt caggagtgag gctaacattc atagaagaac tgttgaaaat atcaataata
    24721 ttattatcct ttgttaccat agaaacagca atcagttttg cattttgtcc agtatattct
    24781 tctgctggta atccaagact tttaataata ttcagataag ttctatcatc aagaaattgc
    24841 atatctactg gtaattcaag catttcactc atagaatgtg aaccctcata gttcaaataa
    24901 tcgtctgtaa gctcacttac tttggcaaca catgaatagg tcataagttt ctgatacaaa
    24961 ccttcgtaaa caccatttgc agtttttagc ttatcgtaaa gcttcaacat ttcgttgtct
    25021 ttcatacctt gtgtagaaaa aataatatcc tggtcagtaa cctctgacct ctgatttgac
    25081 atctgtttta ggtctgctac taaagcactt gtagatataa atagaactat gcttaaaaca
    25141 agcgacaata caatacttcg ataacgtttc ttgtttcttt taaagttttt catagcaaga
    25201 actccctcaa gaccataaac tcgctgtgtt agttttgatg tcttcacaga tttagattca
    25261 atttttacct catttgtctg acgaatacac tccatcactg gtgtgttggc agcctttctt
    25321 gctggtatgt aagcagaaat taagattgta accatactta ccactgctgc aacgatgatt
    25381 gcagggatag acaatgttaa tgtcaacgga acgtttacat agagaatatt tgcaaagttt
    25441 ttagctatga cagaaatcac aagtccaata ctgactatac cgattgcaac accaattggt
    25501 ataccaatcg caccaatgca aattccttca aatagcaccg aatttcgtaa ctgttttgct
    25561 gtagctccaa cagatgaaag tattccaaat tgatgtgttc gctcgttcaa tgacatgtta
    25621 aatgaattat atattagaaa aattgagcct agcatgatta tggcaagcac aatgccacca
    25681 accgagtaca aaaacatatt gaacaaattg ttatcagaaa cacctatgaa gcgcaacaca
    25741 ttgtcattga aaatggaagc atggtcttta gtgctactta tgtaagagcg aatctgatgt
    25801 ggatttttca atgtgacaaa aaggctaaga ctgtcagtct tgacttgttt atcttctttg
    25861 gttatcaagg tatatcctgg tgcagaagat tcttcaaata cgggctttct acagataccc
    25921 acaactgtat aagttttctt atcctgtggc acaaaaactt cactcccagc agtgtatggg
    25981 tcactctgac caagcttttc attttcactc attcgtttcc ctacatccaa agaaatagta
    26041 tcacctactg caaatttgac accaccatct gtcgcaactt tacttgaaac aagaatctct
    26101 ctactatttt caggtaatct tccagaaatt aaagttatag gcaaagtatc aaaagcttct
    26161 ttgctaaatc cagcaataaa aagatatggt ttatctggat ttctgttacc ttcaagtgtt
    26221 gcatagccaa tattttcaaa tgttgcagta tttgcaactt catcattgac tctttgttcc
    26281 tgtacaaaag aagaatccac atccaaaaac tggacatgcc aatcaccata ttttataata
    26341 gctccatttg tcatatagtt catcaaagaa actccaaagg tagtaactgc tgtaatcata
    26401 gcagctgaca gaacaactcc tataattgtt acaagagttc gagtgcgatt ctttttcaat
    26461 ccttgcagag caactttgtt aaaaatgttc attaacgatt caccttcctc tcatcacgca
    26521 ctacttttcc atctgaaata cagataattc tatctgcctg taaagcaatg ttttcatcat
    26581 gtgtgacaat aatgagagtt tggtgatatt tactatggct ctctttcagt aactggataa
    26641 tctcttgtcc atttttactg tctaagctac ctgtaggctc atcagcaagc ataactgctg
    26701 gtgcattcat caatgcacga ccaatggcaa cacgttgctg ttggccacca gaaagttgat
    26761 ttggtaaatg tgtttttcgt tcttttaaac caagcaattc taacaagtca tttaaccgtt
    26821 cttgattgac cttacgctta tccataagta taggcaatgt gatattttct actacattta
    26881 atgtgggtat cagattgtga aactgataaa tcaatccaac ttgtcgccta cgaaaaatag
    26941 caagtttttc attgctttgg ccatatacat cttgcccctc cagatatatc tttccacttg
    27001 ttggtacgtc tactcctgca atggaatgta gtagtgtaga tttaccagaa ccagaagaac
    27061 caatgattgc agtaaactct cctttttcaa tagtaagtga gacatggtca agtgcagtaa
    27121 ccttattttc attcttacca taaaccttac ataaatcttc aatctttaaa aacgccatta
    27181 tataagtctc ctttcatatt ttataaatat atcataggac tttcaactta catttaagtg
    27241 acatttaaat gagagattcg tcactttgga aaacgaataa caaatattgc accaccttgt
    27301 gggtgatttt gtgcagtaat tgttcctcct tgtctcataa taatcgtctt acaaagtgcc
    27361 aatcctattc cataaccggc tgtgcttgaa tttttcccac gataaaatct gttaaataga
    27421 catggtaaat cttctttttt aaagcctgct ccgttgtcat gaatagtaag ttgagtaaac
    27481 aaaaagttgt cctcacaaat gatatcaatt tttccattat cacccacact ctccatgcag
    27541 tttttaaata tattttgaat tgcttctgaa agccaattta aatcacccaa aatccttact
    27601 ccttttggta tatcaatatg taaagtgata ttgtgcagtt ccatgggaat caaaagttga
    27661 tgaagtgcag aactaatcaa attattcaca tcaatttgtt ctttttgaaa gactacaata
    27721 cctgcatcta aacgtgataa ttttaaaagg gaagtaatca gccagtccat tcgtataagt
    27781 aattcctctg tttctcgtat caactctttt ctctcatctt catcagaagt attctctaac
    27841 agtgacaaaa taagagttac tgatgtaaga ggagtgcgaa gctggtgggc tatgtcagct
    27901 agtgagtcag caaggtgttc tttttctttt ttcaatgcgt aattttgctc tctaatacgt
    27961 aacgtcattt tagttatttc actttgcaga atagaaagtt caccctcatc tgattcagca
    28021 atatacagat ggttagcatt atgaagcaca agattaattt gttctgaaat ctgtgcaata
    28081 cttttgtatc gatttttagt aaacacaaaa aatgctattc caaaggtagt ggcagaaaca
    28141 atagaaagta ttccagtcat aatactgatt gcaaatccca gggctacagt gagaatagta
    28201 attaataaga ataaaatagc aaattttcga aactctctat ttcgaaacat aacttccccc
    28261 caatctatac cctgtcccac gaacagtcaa aattatttgt ggacttgaag ggtcatcttc
    28321 tatcttctct cgcaagcgtt tgatgtacac agtcaatgtg ttgtcattaa caaactcacc
    28381 tgccgcatcc cacaattcat caagtaatct taccctcgta ataatacttt taggattgct
    28441 aataaacacc aataacaaac ggtattctaa agctgaaaga aaaacctcat tgccattctt
    28501 tttcacaaca ccggttgccg tatctacata aagcccacaa atttcaaaag ttgacccaaa
    28561 acgcccactt tttcgtagtg ctgttctaat tcgcgcaatc agttcacgtg gacgaaaagg
    28621 tttagtaata taatcatctg cacccatatt cagtccagta acaacacttg attcatcacc
    28681 ggaagctgtc aaaaagataa caggaatgtc ttgcttttct ttgatttctg tgcaaatcgt
    28741 aaacccattt ccatcaggta aagaaatatc aataagtgcc aaatcaaatt tattcttagc
    28801 aatctctaca agtgcttcac ttcttgtgga tacatgggtt actataaatc cttctgaatt
    28861 aagcaaaaga ataagatttt tggcaattgc tttatcatcc tcaactaaaa atatccgttt
    28921 catttacttt catcctcctt tattttatta gttttatata ttccagcaga gcaatagtac
    28981 actactattt tacttatcat tacacaattt tcagtagttt cttttgttct tatataatcc
    29041 atgcataata cctccattga tttattatat ttcttataac ataaatacac aagataagac
    29101 aaaaaacata atgtaagtgc aagtttttta actcactaac ttataaacat acactcctac
    29161 taacactact ttcttgatga cacatcttac aagcttatat tcatacatca atgaaaaatt
    29221 aaatttatcc atttattaaa ataaaaggca atagcttcta tagctaatgc cctttatctt
    29281 aaaatgattt aaatactata caatttttat aaatggctat aaataaactc gtatagaata
    29341 taaaatttat ctacagtctt ttgttataca aaatttataa cctcttatag aacaaattta
    29401 ccactttgca aacttatcac ttttactcag ctatattata aacttttaat ttatctttgt
    29461 ttaaatgttt cttcttaact tctactaaag cttttagcgt atccaaagat gtcattattt
    29521 cagtagaaaa ttcaatggct gttcttctca tcttaaatcc atctcttgta gagtcatttc
    29581 ctttagttgg tgtatttatt actatatcaa cttgtttatt tcttatagca tctaatatgt
    29641 ttggtctagg ctcttcaatt ctatttacta tatcagcctc tattccattt tctcttaaac
    29701 tcttcgctgt tccttctgta gcaacaaaag tatatcctaa ttctttcata tcttttgcta
    29761 tctctataaa ctcatcttta tcatgattat ttacagtagc aagtactact cctctttcat
    29821 cagacatatg tctacctgct gccaagaacc ctttatataa ggcttcttct aaattttctc
    29881 ctacacctag aacttctcct gtagacttca tttcaggtcc tagacttact tcaactttag
    29941 ataatttaga cattgagaat actggtactt ttactgatac taattttggc tctttgtaaa
    30001 ctcctgtact atatcctaaa tcctttaatt tagctcctaa catacactta gtagccaagt
    30061 caactattgg tactccactt actttactta tatatggaac tgttctacta gctctagggt
    30121 taacctcaat tatatataat tcattttgaa attctataaa ttgtatatta accataccaa
    30181 gcacattaag gtctaaagct atttttttag tgtattctac tattttttct tttatttcat
    30241 cagatatatt ttgacttgga tacattgttg tactatctcc agagtgaact ccagctcttt
    30301 ctaaatgctc cattatacca ggtatcaata tatcttcttt atcacatata gcatctactt
    30361 ctatttctct accagttaga tatttatcta taagtactgg atttttatgg tctctatcaa
    30421 atgcatcatc caaatactga attagttttt cttcgttata agttatttcc ataccttgac
    30481 caccaagtac ataagatggt ctaactaata ctggataacc taactcctta gcaatttcaa
    30541 caccttcatt agttgtccat acaccttttc cttttggtct atttatatct agtctctcta
    30601 gtaaatcatc aaatttttct ctatcttctg ctgcatctat atctctaaag tctgttccca
    30661 gtataggtat atttttttca tgtaaaaact tagccaattt tatagctgtt tgtccaccaa
    30721 actgtagtat tacaccttct ggtttttctt tttctattat atttaaaact tcttcttctg
    30781 ttaatggttc aaaatacaac ttatcagatg tatcaaaatc cgtacttaca gtttcagggt
    30841 tattgtttac aattatagtt tctattccca tttttcttaa agattttaca cagtgaactg
    30901 aacaatagtc aaattctatt ccttgaccta ttcttattgg accagaaccc aatactacaa
    30961 cttttctctt atcactaact actacctcat catattgttc ataagttgaa tagtaataag
    31021 gtgatagagc ttcaaattct cctccacatg tatcaaccat tttataaact ggttgtatat
    31081 tatataaact tctcaattca tataattttt ctgggcttat cttcattaag tcagatatac
    31141 ctttatcaga aaatcccttt ttctttaatt catgtaaata ttctttatct aagtcttcta
    31201 tttttagaac ttttaacttt tcttcttgtt ctactatcca tttaaattta tttataaacc
    31261 acttatcaac accagttatt tctgatatca tttctacttt atagcctctt cttatcattt
    31321 cagctaagtc aaatagtctc tcatcatcag gtactactac tctagccttt aattcttcta
    31381 atgttctacc ttctgatgga gcatgaacca atgaatattt tcctgtttca agtgatctta
    31441 tacctttaag aatagcagcc tcaaagttac ttcctatact catgatttca ccagttgcca
    31501 tcatcttagt tcctaatttt ctattggcat gtctaaattt atcaaatggc cactttggta
    31561 tttttactac cacataatct aatgttggct caaaacaagc ataagttttt ttagttactg
    31621 cattttctat ctcatccaaa gtataaccta aagctatctt agctgctact tttgctattg
    31681 ggtatcctgt agcttttgat gctagggctg atgacctact aactcttgga tttatttcta
    31741 ttatcgcata ttctaaacta tgtgggttta aagctatttg aacgttacat ccaccttgaa
    31801 cctctattgc atttattatg tctattgatg cctttctaag tagttgatac tcttcatcac
    31861 ttagagtttg acttggagca acaactatac tgtctcccgt atgaactcca actgggtcta
    31921 cattttccat attacataca gttatacagt ttccatttcc atccctcatt acttcgtatt
    31981 ctatttcttt ccaacctttt atactctttt caagaagaac ttgtccaaca ggacttagct
    32041 gtagtccatg tgaaagtatt tctcttaatt cttcctcagt atcagctata cctccaccag
    32101 ttccacctag cgtatatgct ggtcttacaa ctactggata tcctatctta ttggcaaact
    32161 caagacctgc ttctaaattt gtaactatat cactaactat tactggttga tttatttctt
    32221 tcattacttc tctaaataaa tctctatctt cgcccttttt tatagattct atagatgttc
    32281 ctataacttt tacattatat ttatctaata taccagcatc atgtaattct acagctaagt
    32341 ttaatcctgt ctgaccaccc atacctgcaa gtaaactatc tggtttttct ttttctatta
    32401 ttttttctat aaattctatt gttaatggtt ctatatatat tttatctgca atttctttat
    32461 cagtcattat agtagctggg ttactattta ctaatacaac ttcaattccc tcttctttta
    32521 atgcttggca ggcctgtgtt cctgaatagt caaactctgc agcttgacct attattattg
    32581 gccctgaccc taatactaaa gttttcttta tactatctaa tttaggcata actctttctc
    32641 cttatctaaa caattttcac agttcttatc ttcatcatct atacaatcaa ctttaattac
    32701 tttattttat cttaaattca aatttactca tcaatacatc tatttaatac atctatgtta
    32761 attaaaccaa actttttgat ttaagttatt cttgtttttt agttagctat tctaatgtta
    32821 tttattttac taattctaaa aatttatcaa aaatatagtc attatctttt ggtcctgggc
    32881 aagcttcagg atggtattga acactgtata catctagttt ctcatgtctc attccttcaa
    32941 cagtattatc atttaaattt atatgtgtca ctttcatttg ttcaggaact tctgaaacat
    33001 aataaccatg attttgagaa gttataaata cttttccttc ttctaaatct ttaactggat
    33061 gatttccacc tctatgccca aatttaagtt tagcagtttt tcctccaaga gctaaagcta
    33121 aaagttgatg tcctaagcat attccaacta taggtttttt accaataaga gcttttatat
    33181 tttctataac atcttctagg tcttctgggt ctccaggtcc atttgacaag aatattaagt
    33241 cagggtttat actcaataca tcttctggag atgttgtagc tggaaatatt gttatatcac
    33301 atccacgagc tataaatgat cttaatatat ttctttttac tccaaaatcc ataacagcca
    33361 cttttggacc atttcccttt atatgctcta cttcttttct tgtaacagtt ctaactgctt
    33421 ctgtatttga aaacttatcc aatttatgtt taacatcttc taatttactg tcttctaatg
    33481 taattatacc tttcatggtt ccattatttc taagtatctt agtcaaagct cttgtatcta
    33541 ttccttcaag tccgataact ttattttgct ttaaatattg gtctatatca atttcacatc
    33601 taaaattact tggagaatca cttttctccc ttactatgaa tccctttact tgaacttttt
    33661 cagactctgc tgatgataaa tttataccat aatttcctac aagaggataa gtcatagtaa
    33721 ctatttgacc atagtaagaa gggtctgtta aaacttcacc atatcctatc attgaagtat
    33781 taaatactac ttctcctaca ctttcttcta aatatccaaa tgcctttcct ataaaaactg
    33841 ttccatcttc taatattaac cttgctttca tactacagta cctcctataa ttctctttag
    33901 acaaactaaa gaacattaac taaatataag cttttaaata tctatatcta tttttcactt
    33961 ttacattcta gttttatact tatttacatt tattttttat ctttttctat tatactacaa
    34021 atttcttaaa aaaaagaagt tatctcaaaa gtaaaaactc ctagaaaaaa taacctcttt
    34081 tttttattta ataattaaat tcttaaacta tacaatcaac ttaatttcta ttcagctata
    34141 ttgtaaactt ttaactcatc tttatttaaa tgtttcttct taacttctac taaagctttt
    34201 agtgtatcca aagatgtcat tatttcagta gaaaattcaa tagctgttct tcttatctta
    34261 aatccatctc ttgtagagtc atttccttta gttggtgtat ttattactat atcaacttgt
    34321 ttatttctta tagcatctaa tatgtttggt ctagattctt ctactctatt tactatatca
    34381 gcctctattc cattttctct taagctatta gctgttcctt ctgtagcaac aaaagtatat
    34441 cctaattctt tcatatcttt tgctatctct ataaactcat ctttatcatg attatttaca
    34501 gtagcaagta ctactcctct ttcatcagac atatgtctac ctgctgccaa gaacccttta
    34561 tataaggctt cttctaaatt ttctcctaca cctaggactt ctcctgtaga cttcatttca
    34621 ggtcctagac ttacttcaac tttagataat ttagacattg agaatactgg tacttttact
    34681 gatactaatt ttggctcttt gtaaactcct gtaccatatc ctaaatcctt taatttagct
    34741 cctaacatac acttagtagc caagtcaact attggtactc cacttacttt acttatatac
    34801 ggaactgttc tactagctct agggttaacc tcaattatat ataattcatt ttgaaattct
    34861 ataaactgta tattaaccat accaagcaca ttaaggtcta aagctatttt tttagtgtat
    34921 tctactatct tttcttttat ttcatcagat atattttgac ttggatacat tgttgtacta
    34981 tctccagagt gaactccagc tctttctaaa tgttccatta taccaggtat caatatatct
    35041 tccttatcac atatagcatc tacttctatt tctctaccag ttagatattt atctataagt
    35101 actggatttt tatggtctct atcaaatgca tcttgtaggt attgagataa tttttcttcg
    35161 ttataagtta tttccatacc ttgaccacca agtacataag atggtctaac taatactgga
    35221 taacctaact cattagcaac ttcaatacct tcgttaagtg accatacacc ctttcctttt
    35281 ggtctattta tatctagtct ctctagtaaa tcatcaaatt tttctctatc ttctgctgca
    35341 tctatatcat caaaatcagt tcccagtata ggtatatttt tttcatgtaa aaatttagcc
    35401 aattttatag ctgtttgtcc accaaactgt agtattacac cttctggttt ttctttttct
    35461 attatactta aaacttcttc ttctgttaat ggttcaaaat acagcttatc tgatgtatca
    35521 aagtctgtac ttacagtttc agggttattg tttatgatta tagtttctat gtccattttt
    35581 cttaaagatt ttacacagtg aactgaacaa tagtcaaatt ctataccttg acctattctt
    35641 attggaccag aacctaatac tatcaccttt ttcttatcac taacaactac ctcatcatat
    35701 tgctcataag ttgaataata gtaaggagat atagcatcta tctcacctgc acaagtatca
    35761 accattttat atgctggttt gatattgtat agacttctca attcatataa tttttctgga
    35821 cttatcttca ttaggtcaga tataccttta tcagagaatc cctttttctt taattcaaga
    35881 agatagtctt tagttaaatc ttcaattttc ataactttta atttttcttc ttgctctact
    35941 atccatttaa atttatttat aaaccattta tctatacctg taatttgttc tatcatttct
    36001 actttatagc ctcttcttat catttcagct aaatcaaata gtctctcatc atcaggtact
    36061 acaactcttt tctttaattc ctcaatgctt ctctcctcag atggtgtgtg aactaatgaa
    36121 tattttcctg tttcaagtga tcttatccct ttaagaatag cagcctcaaa attacttcct
    36181 atactcataa tttcaccagt tgccatcatc ttagttccta attttctatt tgctttttta
    36241 aatttatcaa aaggccattt tggtattttt gccaccacat aatctatagt tggttcaaaa
    36301 caagcataag tttttttagt tactgcattt tctatctcat ctaaagtata acctaaagct
    36361 atttttgatg aaactttagc tattggatat cctgtagctt ttgatgctaa agctgatgac
    36421 ctactaactc ttggatttat ctctataact gcatattcaa aactatgtgg attaagtgca
    36481 aattgaacgt tacaaccacc ttgaacttct actgcattta atatatctaa agatgctttt
    36541 ttaagcattg cacattcttt gttacttaaa gtttgagttg gagcaacaac tatactgtct
    36601 cctgtatgaa ctccgactgg gtctatattt tccatgttac atacaactat acaatttccc
    36661 tttgaatctc ttattacctc atattctatt tctttccaac cttttatact cttttcaatt
    36721 aatacttgtc caacaggact taattgtaga ccatgtgaaa gtatttctct taattcttcc
    36781 tcattatcag ctataccccc accagttcca cctagtgtgt atgctggtct tacaactact
    36841 ggataaccaa ttgtaagtgc ataatcaaga cctgcttgta aatctgtaac tatatcacta
    36901 actattacag gctgatttat ttctttcatt acttctctaa atatatctct atcttcacct
    36961 tttttgatag attctactga agtacctatt atttttactc catatttatc tagtatgcct
    37021 ttttcataaa gttcaactgc taggtttagc cctgtttgtc cacccatacc tgcaagtaaa
    37081 ctgtctggtc tttctttttc tattattttt tctataaatt ctattgttaa tggttctata
    37141 tatattttat ctgccacttc tttatcagtc attatagtgg ctggattact atttattaat
    37201 acaacttcaa tcccttcctc tttcaaagac tggcaagctt gtgttcctga atagtcaaac
    37261 tctgcagctt gacctattat tattggtcct gaccctaata ctaaagtttt ctttatacta
    37321 tctaatttag gcataaattt ttctccttat ctaaatggtt tttactatct gtaaataata
    37381 ctaaatctat attaatgcta agaatttatt aaatatacaa tctaaatctt cttttgttgt
    37441 gtattctgga atgtactgaa cactatatat tggtaattcc ttatgtctca ttccttctat
    37501 agtattgtca tttaaattta tgtgagttac ttctgtattt tctggcattt ctgaaacatt
    37561 atatccatga ttttgagaag ttataaatac ctttccagtt tctaaacatt taactgggtg
    37621 attacctcct ctatgtccaa atacaagctt tgaagtcttt cctcctaatg ccaatgctaa
    37681 aatttgatgt cctaaaccta ttcctgtcaa tggctttttg cctattaatt tttttatatt
    37741 ttcaactata tcatttatat cctctggatt tcctgggcca ttagataata agattaagtc
    37801 aggatttata tttaaaattt cttctactgt agtcattgct ggtactatag ttatatcaca
    37861 gttacaagcc ttaagagact ctagtacact atttttaaca ccaaagtcta taactacaac
    37921 tttagctcca gtacctttta tattttcaat ttcttttctt gtaacatttt ttactgaatc
    37981 tttgttacaa tgctcttcta atttagactg aacatcttgt aatgatacat tttctaaagt
    38041 tataattcct ctcttagttc cattgtttct aataatttta gttaatgctc ttgtatctat
    38101 tccttcaagt cctattacct tattttgctt tagatatgtg tctatatcca tttcacatct
    38161 aaaattattt gggttatcac ttttttctct tacaataagt ccttttactt gcaccttatt
    38221 tgattcaaca tcttctaaat ttattccata gtttcctata agtggataag tcatagtaac
    38281 tatttgtcca taataagttg catctgtcaa tagttctcca tacccagcca ttgaagtatt
    38341 aaatacaact tctccaatag cttcctttag atatccaaat gcttttcctt caaatactgt
    38401 tccatcttct aaaattaatt tccccttcat gtaagctacc tcccaaatat tttatctaag
    38461 cattttctat ttctcttaca atattaatag ctgattctct tggattttct gcttttgtta
    38521 taggtcttcc aacaactaaa taatgagcac ctttttttat tgcatctgat ggagttacaa
    38581 ctcttttttg gtctccaacc tctgctgatt taggtcttat tcctgggcaa actgttataa
    38641 aatcagttcc acatgcttct actattttat ctacttcttg cgctgaacaa acaaccccat
    38701 ctattcctga ttcttttgcc agtttagctc tctttatagc taactcttct gttgtcatat
    38761 tacatcctat gtcctgtaaa tcttggttgc ttaatgaagt taaaactgtt actccaacta
    38821 ctaatggctt ttctatattt aatctttctg cttcttccct agcaattagt gcacattgtc
    38881 tcattccttc catatctgat acatgtattg tcattaacca aacattatct ctcactgcag
    38941 ctttcactgc acttttcata gtatttggta tatcatggaa tttaaggtct aaaaatattt
    39001 ttttaccttt ttctttaaga taatctattg ttttaccttt tgtggcaacg tattgctcta
    39061 gccctacctt aaatatgtct acactatctt ctaatctgtc tattagttct ttagctttat
    39121 caaattcgtc agtatcaatt gcaactatca atttttcttt accatttatc atttttacat
    39181 ctccttttta ataaagccta aataatcata aaaaaatcct cacaccaaaa gaggcataag
    39241 gattttatct atacataact ctattcatta gctatgagat atctttccca gtctctctgg
    39301 actagtttaa aagactttat tatatttaaa tttttatttt atactcatca aaaacctcta
    39361 gaagatttac tagaggttat atactaacat tatctataac cctttagtct ctctggacta
    39421 ctttaaggaa tttagctgta atatttttct ttatttatat aactttatgt atctatagga
    39481 taatttaaag ttttatcttt tttataatta aaccactaat tgctttttat gtcaataagt
    39541 ctttgaatta ttcttcatct ttatcaaaca attttttctc aaaatatgat tctgcattat
    39601 atattgcagc aactggatta tgcccagtta ctctatcttt gacaactaaa gttgttatag
    39661 gagcttccat atatttcatg gctaaagtat cgtgaccaac acataatcca agaattacat
    39721 taaggtcaac cttagcttca tttaaaaata aagcttgtcc tattggattg cacattacct
    39781 catctgaaca ctttgatatt gtttgagatg cttctagtcc aattgtactc ttaggcattg
    39841 ccccattttt acatataaca gaaaatactt caaacccatg atgttcaaat attttttgca
    39901 ctgtttgcat ttctcttttt agtccaatac aaaatgctat gccaatcttt ttataaccac
    39961 actttttagc aaactctata atctctttta gtctagtata ttgacaatac ccctctgctt
    40021 caactaatcc agcattataa gctatttttc tattctcttc ttcttcataa agttgctttg
    40081 ctttttgttg tagttcaatt tctctagttg gacatatctt cattaattta tccatatttc
    40141 cattagaaca gcctctaaga ttacattttc cacacttata catatccaat atccccttcc
    40201 aaaattttat tcatcttgcg attcttcttt ttctagagtt acattttctt cttgggtttc
    40261 ttcttctggt ttcacatgct cgactacagt tataaaatat gattttccta ttttattcct
    40321 tataggaagt ttttcatatg aaacatctat tttatttttt atacaagcta tatcaaaatg
    40381 tcttaaggct acacctaagc taaccttatt catatcttca cagtgtttct taggtctctt
    40441 ttcaaataaa tgaagacctc gttcatcctt atggaaatac cacggttgag aattttttac
    40501 agatggagct cttcttgtca attctaagat atctttccac tttctattcc tatttatctt
    40561 atttatcata tcttttactg gctttctatc aggtctagca tatctcgtcc taaacaactc
    40621 ttcatttttt gctggatatc caaatgctat tattatataa ggattttcta ttttgttttc
    40681 atcatcttct tctgttttta ttccagctag ataatctaaa tcatcttcct catcttcttc
    40741 aaaattctta ttcccaatat ctacaaattc caagatatct tctctcttta agtttccttc
    40801 caaccagcaa gtagctattc ccaatgttgt aaggcgcaaa actacacctt ctgtagcaaa
    40861 tcctatattc tgtaaatagt cctcaccttt gtctgatgta actactatat aatgaggtgc
    40921 ctttacttta cattccttac ccatcaaaaa atgtattata tgacctctat caacaacatg
    40981 tgctttaata tttaaatctt catttaagta ttctaaattt ttacaacttc tttttatttc
    41041 ttccatcaat ggctttttta tcttcttact ggaaaaattt cttacagatt ttctataaaa
    41101 aatggcttca taaagctcca tttatgccct cctaactgaa atcttctgac gtataaactc
    41161 tataaattta tataaatact ttatattttt tatagtttta tttccttttt cctcctgccc
    41221 tattttgaaa ttcataagaa tatctactct agtttaatat agcattcaaa aggcttaaat
    41281 tctaccttat caatgtctat acattagcat aattttttta tttgattttc tatgctaatt
    41341 taaataacaa cactagctat taccttttta attaagcttt gccagaaaaa ttttttttca
    41401 tttataaata tctatctcat tattatatta taagtttttt tcggtattct tgtatagctt
    41461 ttcactaaaa aagtattttt atttctactt taatattatt atcaacaaaa aacatactgc
    41521 ttaaataagt ttgttatcct cataatttgt tttaattaaa atattttttt tatttctcaa
    41581 tctataaagt ataaatattt tgaatatgac ccaaaatcta agtaaagtta taatatttta
    41641 taagttgttc aatattaaaa acataagtaa tgttataata ctttataagt tattcaatat
    41701 taaaaatata ggaggataaa tatgaattta aatagaaatt tagaaaaata cgctgaatta
    41761 gcaataaaag ttggagttaa catacagcca ggtcaaattc tacttataaa gtcaccaata
    41821 gaatgtgctg attttgcaag aaatgcactt aaagaagcat ataaatgtgg tgctaaaaat
    41881 gtttatatag agtggtctga tgaagaaagt actcttatta aatacttata tgctccagat
    41941 gaagcatttc atgagttccc caaatggaca gcagaacaat atgtagatat agcaaaagaa
    42001 ggtggggcat ttttatcagt ctatgcccaa aatccagatt tattaaaaga tgttgaccct
    42061 gaaagagttg caaattttca gaaggcatct ggaaaagctt taaaagaatg gcgttcttac
    42121 actctaactg ataaatgtaa atggaatata gtctctgtgc caacaaaaga ttgggcaaaa
    42181 aaagtgtttc caaatttatc agaagataaa gcaatagaaa aactgtggga tgctatattt
    42241 aaatgttcaa gagtagatgg tcaggaccct ataaaagcat gggaagaaca taatgaaaat
    42301 cttaaatcta aaatggactt tctaaataaa aataacttta aaactttaaa atataaatcc
    42361 tcaaaaactg atttgactct agacttgcca aagggtcatg tttggctaag tggagcatcc
    42421 aaagacccta atggaataag ttttaatccg aatattccaa ctgaagagat ttttggaatg
    42481 ccacataagt ttaaagtaaa tggaactgtt tatagtacaa aacctcttgt ttatggtgga
    42541 aacataattg acaatttctt cttaacattt aaagatggta aaataataga tttctctgct
    42601 gaaaagggac tagatacact agaaaaactt attgaaacgg atgaaggctc tcattattta
    42661 ggagaagtcg ctcttgttcc atacaactca ccaatatcca atacggatat aattttttat
    42721 aatactctat atgatgaaaa tgcttcttgc cactttgcaa ttggttctgc ttataagact
    42781 tgtctggaag gtggaaataa tttaaaagat gaagaattgg atgaacatgg tgtaaatgac
    42841 agtttaacac atgtagattt tatgattggt tctgctgata tggatattat tggtgagact
    42901 tatgatggaa agcaaataca gatttttaaa aatggtaatt gggcttttta acatatgatt
    42961 ttaactaaaa agggctgatg gaagttatac cagacttccc agcccttttt aatgttgatg
    43021 ataaatattt tttatattaa ttaatctcat tattatagat taattcctat tgttttaaac
    43081 ataagtatct aaaacttata tttatattat atataaaata tcctatttag taaaaccttt
    43141 attttaattt atatgctaaa aatttaactt tgctatttca acagcagact ttgcatcctt
    43201 tgaataataa tcagcaccca tttcttcagc gtattcttct gtcaaaactg cacctcctac
    43261 aaataccttt gcatttatct cattatctct cagtgcctga attgtatctt tcatactttg
    43321 aacagtcgta gtcataagtg cacttaatcc tactaatttt atatttctct ttttagcaac
    43381 ttccactact tcttcaatag ggacatcttt acctaaatct ataacttcat atccataatt
    43441 ttctagcatt atttttacta tatttttccc aatatcatgg atatcacctt ttactgtagc
    43501 aactattatt tttcctttgg atacattatt gctactttta cttaataaag tttcctttat
    43561 tgtatttaat gaaactttta ccgtttctgc tgattgaatc aattgtggta aaaatatatc
    43621 tccactatca tatctttttc ctactttatc aagagcagga attaaaatct catctaatat
    43681 ataattttca tccttctctt ttaaaagatt cagtgttaca ttttttgctt catcttttaa
    43741 tcctctttca actaaaatat ccaagggcaa atctttatca atctttgtag atgaatccga
    43801 tttttttgaa ttatttatat tgctatattg atttatatag tttatacatc ctttatctgt
    43861 gttatttaat attttaaaag aattgatagc ttccatcata ctatattcat ttggatttat
    43921 aattgctaaa tcaagccctg cccctaaagc taatgttaga aaagaagcat taagtgcttt
    43981 cctatttgga agaccaaatg aaatatttga aacacctaat atagttttta ctcctagtgt
    44041 ctttaccatt gttatagctt ttatagtttc tattgcttct tcttgttgtg ctgaaacagt
    44101 taatgataaa cagtctataa atatatcttt tggttttatc ccataagaaa ctgctctatt
    44161 aactatcctt tttgctattt caaatctttc ttcagccttt ttaggtatcc ccttttcatc
    44221 aagtgtaagt ccaaccacac atgaaccata ctttttaact attggtaata tactttctaa
    44281 agactcctct ttgccattta cagagtttac tattgttcta ccattataat atctaagtcc
    44341 ttgctctaag gcctctacat tggaagaatc aacttgtaaa ggtgtatcta ctactgcttg
    44401 aatctctctt attaattttg gcattagttt tttttcatca atctctggaa gaccaacatt
    44461 tactccaagt atttgtgctc cagcatttac ttgctctaaa cctaaattta tagcataatc
    44521 aacattttca ttttttaatg cttcttgtag agattttcta cctgtgggat tcaatctttc
    44581 tccaacaact gtaggcgatt gtacttccac aaattttgat ggtgaacaga ctacacattt
    44641 atcaattttc tcgatacatc cctttgtaac agaatttata ttctcagata tttcttttat
    44701 aaaacttgga ttcgtaccac aacatccacc tattatagta gcaccaactt caacgaactt
    44761 tttaacccct tcaaaaaatt cttttgcatc gacatcatat atagcctctc catcaactat
    44821 atttggaaga cctgcatttg cttgaaccat aacagggact gtagctcttg atgctatttt
    44881 ttcaactatt ggtaaaagtt gtttaggtcc taatgaacaa ttaactccta ttgcatctac
    44941 acctagacct tctatagtag ccaccataca ttctggcata catccagtaa aacttcttcc
    45001 accttcatca aatgtcatag tacagaaaat aggtaaatca gagttttctt ttgctgctaa
    45061 tacagctact ttagcttcat ataaatccat catagtctct attactatta catcaactcc
    45121 agatttaacg ccttgtagta cttgtctttt aaatatttca tatgctttat caaaacttaa
    45181 agtacccata ggctctagca tttctcctat aggaccaata tctaaagcta catatcttgg
    45241 tttagtatta tctacatttt ctatagcttt tctagccacc aaaacagcat tgtcaatcac
    45301 ttcttctaca gtatacttac tatctaattt tagttcatta catccaaaag tatttgtaag
    45361 aattacattc gaacctgcct ctaagtatgc tgtatgtatt tctataagtt tatctggatt
    45421 ttgaagacca aatacttctg gattttcacc taactttaac cctttttgtt gaagcattgt
    45481 tcccattgca ccatcaaata ttaatatatt atttttcaga tattgtctta tttccacaag
    45541 agttcgctcc ctttctatat gtacagtttc tatagttttt acaattttca cacgactttt
    45601 ctttcttaga tttatttatt cttgtattag aaatacctat aatagccact actgactttc
    45661 tcggtatcat aattccatta gaagtaattg ttagacctat ttgtttttga gcattcaaaa
    45721 cgtttaatat atctatattt ttttctattg gtaaatctcc atatcctggg ctatatcgca
    45781 tagttataaa tttatcttgc tttaaaaaaa ccttttctaa attattttga acaatgtcac
    45841 acacttcttc tattgctgtt gttccacatg aatcaattat tagtcctttt gttaaatttg
    45901 aataagagtg tcgtcttatt tctttttcta tatctatccc tatagtagct gccataatta
    45961 cacactcatt acattctttt aaaagttcat atacatattt actctctaat gttaaatttg
    46021 ttccctctaa ttctacaaca ttatctttat ttattctaat aggataaatc ctagataaat
    46081 atctaggatt tattttattt ttagttatag ctacacattc atcaatagta tatttaaggt
    46141 ctttatctat ctcttgacct ctgtattgta aatatcttaa aacttcactt tgatttatgc
    46201 ttatatcttc tcttccaatt aaattattct ccatatatat ttaaagaaaa tcctttatct
    46261 aaaacatctt ttgcatattc ctttgcccca aataaagata agtctctgta gtttccacat
    46321 tctatagctg tagctgctgg cattttatct gattcaagaa ctttttttaa tgcttcttct
    46381 aaaccaatct taactgtttt ggcatctaca tctccccata gtattaaata aaatcctgtt
    46441 ctacatccca ttggagataa gtctattaca ccatctaatg tatctcttaa ataagtagct
    46501 aaaagatgct ctaaaccgtg catagcagca gttccaaaac tttctacatt tggctgtaaa
    46561 aatctcaagt caaatttagt tactttatct ccctttactc catcaagtac actacacttt
    46621 cttacaaaag gagcttttac ttttgtatgg tctagcttaa aactttctac tttttccatt
    46681 atttaatcct ccattatact ttaaaatttg ttacattaat tctaaaaaaa actcttccta
    46741 tctattattg ataagaagag tatattattt caagtcatct catcgttgtt agcaggacca
    46801 cctgataaaa ggttggtata ggatcactga gctaactctc tcccctaact cttgataagt
    46861 atcttattaa actgacttaa attatatatt acttttttat ttttgtaaac actttattgt
    46921 tataatattt atatcacata atcgtacttt tattttataa ctaagctgat tgtattattg
    46981 gcagttgtac ttcaaacaca gtttgatatt tatcgcttga aacagtaatt tctccatcat
    47041 gtaaatttac aatatttttt gtaatcgcaa gacccaatcc agaacctcca acatttctat
    47101 ttctcgattt ttcaactcta taaaatctat caaatatatt tggtaaatct aacactggaa
    47161 ttggttcccc ataatttatc acttttacaa ccgccatatc ttcctttttc tctgtgacta
    47221 tatctacata atgcccatct tttccatatt tcacagcatt agttataaga ttttcaaatg
    47281 ctcttaccaa tttatttgga tcagctttta caattaattt ttcttcatta aaattaattc
    47341 tactaaccat aaattcttgt ttaaaataaa tttcaaattg agatactact tgacttaata
    47401 actctactaa atttatttct attttgttta attttattgt attattttgc attttagtca
    47461 attcaaacaa atcatttata agaacattta aatttttcga tttttcatat gcaatatcaa
    47521 catagtacat catctgaact tcatctttat acttaccttc ttctatcact cctaaataac
    47581 ccattatgga tgtcaaaggt gttcttaagt catgtgacac atttgttatt aaatctgttt
    47641 ttgtctgctg agcttgtctc tcttcaatag ttatatttct aagttgatgg actatattat
    47701 tgattttatg tgctagttca tcaatatcat ccttttcttt atcctttaat atatctaaat
    47761 catactcaat atctatactt ttttctaagt taccttcatc cattaaattt acagcacttt
    47821 taatgatttc taatttttga ttttttctat tagtctttct tataaaatct acaatttgct
    47881 tatttctctt gtacgatata atggaataaa ttgctaatga tgtgattgta aataaaaatg
    47941 gataaaataa atcataccct ataagacttc tgaaatctct tgcttgatat acaagttgat
    48001 taactacaga catatcacta ttgacatata atgattttat tatatacata cttgttaaaa
    48061 taagcattga agttaaagca cagcttatta taagatctaa tatgggtcga aacatatgat
    48121 ttaataaact tttatttttc aattttatat cctactcccc atacagtatg aattattttt
    48181 tctccatctg tatatttttc aattttatcc cttattctgc tcatatgaac cattacagta
    48241 ttattagatt ggtaatattt ctctttccat accttttcaa atatttcttc tgtactatat
    48301 actctgcctt tattatttgc tagtaaatat aatatatcaa attctcttga tgtcaaaatt
    48361 atttcatttc cagccactgt tacactatgt ttttccttat ctataattac tactccaacc
    48421 tttatgacat ctttatcttc attattagca ccactaaaat aatatgctct tctaatcagc
    48481 gacttcactc tcacagtaag ttcaagtggg ttaaatggtt ttgtcaaata gtcatctgca
    48541 ccagtcatta tcccttgtat tttatccatg tcttctgatt ttgcacttaa cataagtatt
    48601 ggtatattca aactttctct aacttttcta caaacttcta tcccatccat tttaggcatc
    48661 attatatcca atatcattaa atgaatattt tcctttttta aaatatctag agcctctttt
    48721 ccatcttcag ctttaattac attatatcca ctatttaaaa gatatatctc tataaggtct
    48781 cttatttcct tttcatcatc cacaacaaga actgtattat tcatttttac acatcctaac
    48841 ttaatctttg tatatagctt tattcatata aaattatact atattttagt tagtgtaatc
    48901 tataaattta ttaatttcta aatttccaat ataatacaaa ttatttacac atttatatta
    48961 tttattttgt aaatattctt ctaaagttaa tccttttgaa taaatttcct cagcagcatc
    49021 cttaccaaca tatctaatat gccatggttc atatgctgta cctgttatat cttctttacc
    49081 ttcaggatat cttattataa atccaaactt gtgagcattt tctgccaacc atttagcttc
    49141 ttttgtactt ccaacaaacc atctttctgg atttgttaaa tcaatacaaa gtccagtttg
    49201 gtgctcactg taaccaggct ttgctacata tgcatctgcc ttttctcgcc cctgagattt
    49261 tacccttctt gtataagtat ccagttgagt atcataagat ctgtatgctg aagaccctaa
    49321 aaactgtatt ccttctgaat tagcttgctt aactaaatct tcaactgcct ttgcaggttc
    49381 tcctgccatt tgtttttctt cctctgttgc ttcttcaaca aaaggtatgt ttaccttagt
    49441 aatattttca ggagtatagt ttttgctaat tccatttgtc ttgtttacca ataatatatt
    49501 tgatatacta tttgagtgat ttttattttg agaatttgcc tcaacatctt gttctgaatc
    49561 atgttgagca gctactattg gatctttatc tttcttaagt aatctatctt tagatataaa
    49621 tacacttata aatactacta aaataatggc tcctattaat ttaaattttt tcataattaa
    49681 atcttctcct ttttgttatg tttgctataa tgttaatttt agagtataag acttaagtat
    49741 tttataagta aatcttaaat aaatcttaaa tttaatattt ttaaaggatg gtgataaaat
    49801 gtctatacaa aaacttctaa gtaaagctcg taaggctata caagattttg atatgataca
    49861 agaaaatgat aaaattgcgg ttggactttc tggtggaaaa gatagtttaa ctctacttca
    49921 tatacttaaa agttatcaaa gattttctcc acaaaacttt gaattgatag ccataacatt
    49981 aaatactgga ggtgttgata attctccact agataaattg tgtaaagaaa taaatgttcc
    50041 tttttatgaa tttcaaactg acataaaaga aattgtattt gatataagac aagaaaagaa
    50101 tccttgtgca ttgtgtgcta atttaagaag aggtgctttg aacgataatg ctaaaaaact
    50161 tggatgtaat aaagtagctc ttggtcatca taaagatgat gcaattgaga catttttaat
    50221 gtctatgttt tatgagggta gagtaaattg tttttcccca aagacatacc tagacagaca
    50281 ggatttaaca gttataagac ctatggttta catagaagag tatatgacta agaaaatttc
    50341 aaaagattct aactatccta ttatcacaaa tccctgccct gcaaatggtc atactaggag
    50401 agagtacata aaaaatctta ttgctaattt aaataaagag atgcctgatt ttaaaagaaa
    50461 tgtatttggt gctttaaata attctgagaa attgtttata tgggataaag agaagataaa
    50521 aaattttaaa taataattaa aatttttaaa taaaaaagca agattgtatt aattgtaaat
    50581 aatctttata taatctacac aatcttactt ttttatttat tatatgttat tatctttaat
    50641 ctatatttta atagttctta atagtattta ttttgtctaa tacattaatt ataacttaaa
    50701 tattttatat attaatttaa tctattaatt cattcaatct actggtttaa tccattttta
    50761 cagtatatac atctacatat tataaaaagc aaaattatat tttctaaata ttttatgtta
    50821 gattataata ttaaattcta ttatctctgg tatattaaaa aatctaaatg gtaactttgt
    50881 acttccaaga cccgaactca catataaatg atttccattt atatcataaa accctttagt
    50941 atatttcctt ccatatggag gagtaactaa tgctccctta ataggtaatc ttatttgacc
    51001 accatgacta tgcccagata gaactaaatt cattgtatct ttactaagca tatctactaa
    51061 atctggttca tgtgctaaca ataaattaaa attcttatta tctatttgac tctccagata
    51121 ttttatatta ggatttccat ttaatatttc atcaacacct aatatattaa tatatttatc
    51181 atcttttaat tttatttttt tattttcatt tactaaaaga ttgaagttgg aatttttcat
    51241 aatctgccta taatacctag gaagtttata catataatca tgattgccaa atactgaaaa
    51301 ttttcccaac ctagcattca tacttgataa aatagttgta atttcatcta cacttggatt
    51361 tttattggag taatcaatta aatcaccagt aaatactatt atatctggat tcaaagtgtt
    51421 aatcttatta gctacttttt tgagattctt agttgaataa aaactaccta tttgagtatc
    51481 tgaaatttga actactttta tcctttcaga tttactctca attgtatcac tttttatcaa
    51541 tgttccattt tcacttatat tatatttttt cacaattaat agactcggct ctatcgcaac
    51601 tgagtatata aaaatcaaaa taaatattaa gataaaaaat tttaaaaaat acttaatttt
    51661 tgagttcaaa atatctacct acttatttat ttttttaaac tatttgcatt acagactcaa
    51721 ctttgttcat ttttctgtca tgcatttttg gcttatttgc tgaaaaccat ttatatgttg
    51781 ctgtaagacc ttcttttagt aacacatttg gtatataaag cccatgttca ataagcttat
    51841 ttatatcaag attaaagtct atatttctaa atgggaaata cgttctttct ctaaactcta
    51901 ctttttccat atctacatat tttattatag gttctttgcc aataatttct ccacatgtat
    51961 atataaggtc atcccaagaa attaactgtg gatttgtcac attataggcc tctcttacat
    52021 gaggattttt cattatgctt tccaatacct ttaccaaatc cccaatatat ataaattgat
    52081 tactaacttg ttttcctttt ggtactggta tcttttcatt tttttctatt ttttcaaaga
    52141 aataatactc tctatataaa ttattatttt caccatatat ataagttggt ctaataatta
    52201 tatatggtat agggctattt ataataaaat cttcagcctc ttttttattt aacccatatt
    52261 ttccccagtt ttcattttca cctttctcat tttcttcttt tatatttctt ccactatctt
    52321 tatatactgc tcctgctgac aatactatat acttttttaa gttatccata cttataaaat
    52381 caatcagatt acttacatct tcttttgtat atgctgtcat atcatatatg taatcatatt
    52441 ttcttcctgt gataatgttt tccatatctt ttcgaacttt tctatcacaa attagatgtt
    52501 ctttaaaacc attataatct atttcctttt taccatttgt aagtatatca atttgatacc
    52561 cacatttaat caagcgctta gcaagtgcag aacctatgaa gtcgcttcca cccataatta
    52621 aaatagattt cattttaagt gcatcctcct cttttttaaa tgacttcctt aactataata
    52681 tcatattttt aaatatgcga caccattttg taatattttt gtctataaaa gatatttata
    52741 acaaaatatc ttttatagac tctacatttt cgacaatata agtaggttca ctttcactaa
    52801 gttcttcaaa agaaccatac ccatacaaca ctccaataga atctatacct atttttttgg
    52861 ctccaattat gtcatacttt ctatctccaa ccataataac tttatcttta tcttttacat
    52921 tacataggtc tagtacatat tgtataactt catttttatt cactcttgta ccatcaagat
    52981 tgctacctgc tatatattta aaatatctat ctatatcaaa atatctaagt atagtttctg
    53041 caaaaactgt tggcttagaa gttgctacta ataatatttt accattttta tagagcattt
    53101 ctaatatctc ttttatattc tcatagattt tattttcaaa tatcccttta tcagcaaaat
    53161 attctctata cttttcaaca gcttctttag tttttttatc ttcaaattta taatactcct
    53221 taaaagtatc atgtaatggt ggtcctataa attggtctaa gttttccaaa tcttctttta
    53281 tgccaaagct atttaaagaa tactgaatag actttgttat tccttcttta gggtctgtaa
    53341 gtgttccatc taaatcgaat aatactattt cataattttt tttcatattt cagtcctcca
    53401 tatttgtaaa aagtaatatc ttatatatta agtttatcta ttttaaaaag ctatagtaat
    53461 agaaatttct aatactatag cttaagtacc caattaaatc ttatatattt attttaaaaa
    53521 taaatttaaa cagctctatt taactgttat agctgaaact ggacatcctt cttcggcttc
    53581 tttacataaa tcttgtaagt ctgctggtat ttctccttca attgctactg aatgcccttc
    53641 atcgctcata ctaaaaacgt ctggacatgt tcctatacat gctccacaag ctacacatac
    53701 atccttatca acaaatgctt tcatactaaa atacctcccc taaaattatt attttttaat
    53761 tgttgtttcc ttagatattt atccaatata taccaattta aacatatatt tattatttta
    53821 tcactattgt ttagtttatt atagtatttt cgtatccatg tctataaact tcactgcttt
    53881 tcatccatga ttttacagta tattgcaatc ttaatttttt atttaaaaca agttcattat
    53941 tattgtagtc ttttataata tcattacact cctccattgt gaatattgta aaatcacctt
    54001 ctccaccaat ctttattctg cctttatttc catgtaaatc gaaaatcttt gttgggtatt
    54061 cagttacttt ttctatacac ttttctaagg atatcccaag ttccataatt ttatttataa
    54121 cacttaaaag agaatagttt acgtctttca tgttttttat atgcatatca gtacttatta
    54181 ggtctggctc taaaccttgc tttattgctt ttttaaaaac tttaaaatca aattggttca
    54241 atccatgaca tacatcaaac aatactcctc tgttctttgc atttattact gattttctta
    54301 atgtgccaga ttctttaagg attccatttg tactatttcc atatactggt gtaaccacat
    54361 ctccttttcc caaaatattc attacttcat ctatatatga tggatactct cctatatata
    54421 ccactaaggg taaatttaat tttttggcaa cttgctttga tttcttaatt atatctatac
    54481 cactttcatt agattgaaat ttactcgctt ttacttttat accctttatc ttttctatat
    54541 ttttttctac aactgactca accttgtcaa aatttataac atctaaagaa gttgattcat
    54601 tcaatatatt tagtccctca tttgtaacat ttaaaaaaga atatacttct gttttacact
    54661 catccatata ctttttttta aaatcatagt agtttaaagg accagctgta cctgcatcaa
    54721 atataactgt agaacctttt tcaattccaa ttgaatcaga atccatacct gtaggtaatt
    54781 ttctcttata acaatgggtg tgaacatcta taaatcctgg ggaaatatac atattatcgg
    54841 catttattat ctcgaactct ttatttgtaa gtaatttttc ttttcttatt ttctctttaa
    54901 ggttcaatcc aatagcatat attttcccat gtaaaaccaa gatatctctt ttttcaaatt
    54961 tgataccatc tgttggagat attattgtcc catttcttat aagcaaattt ttcatttatt
    55021 ccacatccta attacaatat aatttctcta atagcttttc ttacataaag aaaataagcc
    55081 taaaaataaa aatattttag acttattcac tatattatac tatattaatt atattaaatt
    55141 aaataaaatc tttaatttac tttaagaagc aattaagtat ctgttgttcg gcttcttctt
    55201 cagtaagacc taatgtcatc atctttagta tttgatctcc tgcaatcttt ccaatagctg
    55261 cttcatgtac taaaagagca tctggatgat ttgcagcaat ttcaggtata gcactaattt
    55321 gagaatttcc cataattatt gaatcacatt gaacatgtgc agaacaaagg gaatccccaa
    55381 taacacaagg attaaacttt tgcttagaat tatcttgccc aactgtacga gaaactactt
    55441 gagcagaaga gttttccccc tctaagataa ccttcatatt agattctgca acttgattac
    55501 catgagttaa taatttctca aatacaacta ttttagctcc tttacctagg tttgcatcca
    55561 tttctctttt agttgaatct acacccttaa tttgaattgt ttccatttca caatatgaat
    55621 cctcatccat attgacaata gtaactggat ttaaaattct ctttccttct ccatcacctt
    55681 caccataatg tttttctaca tattttaggc gagctccttt acgtaagaaa aatctatgta
    55741 taccattatg ttcactatca tcacaaccac agttatgtat accacaccca gcaattataa
    55801 caacatctgc attttctcca atttcaaaat cattatatac catatcctgt acaccagtct
    55861 ctgttactat tactggtata tgtacatttt ctccttttgt atttggcttt actataatat
    55921 ctattccagg tttatcagtt ttaggaatta tagttacatt ttcagttgaa tgtctctcta
    55981 ctccttgccc atttttacgt atattgtatg ctcctaatgg agttccacga agttcagcaa
    56041 ctttttctaa aagatttaaa tctattttgt tcatttaatt tgccttcctt tctaattaat
    56101 tcatgtcttt cattaattca caacttgaat taacttgaca catgatttca ggtaatatag
    56161 cctcttttgt tccatggctt tttactgaac catcttgtag aacaattatt tcatcagcta
    56221 actctaatat tctttcttgg tgagaaatta tgattatagt ctggttagac tcttcatgta
    56281 ttttcttaaa actttcattg agtttcccaa agctccaaag gtctatccct gcttcaggtt
    56341 catcaaatat agaaaccttt aaatctctag caaaaagagt tgctatctca attcttttca
    56401 tttcccctcc agaaagagag ttatcaactt cacgatttaa atagtctttt gaacacaatc
    56461 caacatctgt aagatattgg caacatacat cagtagacaa tggttttcca tgagcaagtg
    56521 taagtaaatt ttcaactgtc atacctttta ttcttggtgg ttgttgaaat gcatatccta
    56581 ttcctttttt tgctctttct gtaatactaa gatttgttat atcttctcca tctaaaatta
    56641 tctgaccact cgtagctttt tcaattccca taataagctt agcaattgta gattttcctc
    56701 caccatttgg ccctgtgata acaataagtt ttcccctctc aaatgaaagg ctaacatcat
    56761 ttattattcc tagttcctca ttgttagact caacattaaa ggaaagattt tttatttcaa
    56821 gcataatacc cctcttttct ataaaatatt acattaatat ccattctttt attatatcag
    56881 taatttttat atatcagcaa ctctaagcag ataaaatgta tattaaataa taaaacaaag
    56941 ttattgaaaa gtaaaaaaga tgagttttat gtaaactcat ctcagaaatt tagtatatca
    57001 acttatatgt atttaaaaat tagccttcta tatactctaa tattattttt tgattatctt
    57061 ttaacataat ttcttctttt ttagattcta ttccattctt ttttatcttc acctcagcta
    57121 caaaggcatc attaccttca accatttcta atagttcata agctttttta cgacctattt
    57181 tagttgctat atcttttagt actgccattt tcttaaacat tttatcacct ctactcaaaa
    57241 tttacactta atttttaatt atataattct aatcctagac tagtttataa taattattgt
    57301 ctctaattat atcagatatt gagtaaattt aatactttgt aaaattattt atcctctctg
    57361 accaaataat catttccatc ttggtcttta aaagaaaaca tccttccata tggcatctcc
    57421 attaattccc caacctcaac ctcgttattt gccatttgtt cataagttcc attaatatct
    57481 cttgtactta aaattattga tggatgagat acatttatct ctttgttttg tctcatcatt
    57541 agatttttat catacaacac aaatgttgta aattcatttt tacttggtgc tacttccacc
    57601 catttcatat ttggacccat ttccttttca taagttacta caaagttcaa tttttctgtc
    57661 caaaacaact ttgcatcctc ttgattattt acatagagag ttatttttcc tattttattt
    57721 atcataacaa tacccacctt tattattaaa taaataccta atatttttta tatttaataa
    57781 tatacccata tatattattt tctatagata tatatttcta atatttatcg ccaaattatg
    57841 taattctgct caatatataa tttatagtat ccatactatt ataggcttta tttatgtaat
    57901 ttaatcacct tatatatcat acttataatt atgataataa ctacataaaa aatcacttct
    57961 aaatagagtt atccatctaa aagtgatttc atatattcta tgcaactttg tgttttagcc
    58021 actttgttcc tcttaatctt aaacaatata aagtccctct aacaagccaa tcagtataca
    58081 tacctatcca aattcctaac actccaaatt ctaatacaac ccccaatacg taacctgtaa
    58141 aaattctaaa aatccacata cctattactg cagtcatcat tgtatatcta gtatcaccgg
    58201 ctccctttag ccctgctgat aatacaaagg atataggcca tattaccata gctaaacaat
    58261 cacttcgaat taattgtgtt gagatgtcaa ttacactagc attttgagta tacatacttg
    58321 aaatccaagg tgcaattggt ataaatatag ctcctactat aaataaacaa attgttgcaa
    58381 atttagttaa atataataaa gtactcttag ctcctttaat atctccacgt cctatatact
    58441 gtcccaccaa ggttgtagca gacaaagcta aagcattccc aattacattt attatggatg
    58501 caattgacat tcctattgca ttggatgcaa ttgctgatgt tcccatagta actataaata
    58561 cttgaacaat caatttacct gcattaaaaa gtagttgttc aactccagct ggtattccta
    58621 tattaaatat agacttttga gtttcaacat caatcttaaa tggaaatatt ttacccatag
    58681 atataaattc gtttccttta aataaagctc ttataattac tatacatcca attattctag
    58741 ctatagcaat agatatagct gcacctgtta tgccatagga tggtgtttgc aaatttattc
    58801 ctagaggatt taaatcaata ccatatataa gtatatatcc taatgttaca tttactatat
    58861 tcataaacat agtcacatac ataggtttct ttacatctcc acaccctctt aaaagaccat
    58921 ttgctatttg ttgtgtcgca ataaatggat acgtaaataa tgtaaactct atgtataatt
    58981 ttgcatttga ttttaccaat tcttctgcgg aaccataaaa taaattaatt acaccagttc
    59041 taaaaaccca catcaatatt gttattgtaa aggataatat tactccagac actagagcct
    59101 gcttaatagt ttcattcacc ttttcaccat ctttttttcc tatttgttga gctacaacaa
    59161 cagtagctcc aacagatagt gcagcaaata cagatataaa tatattgttt attgaatcaa
    59221 ccattccaac tgcagataca gcttcttccc caatatgacc tgccatcatt gtattaaata
    59281 ctcctaaaag catgacaaac gtctgctcta tcattatagg aattgctaac tttataacat
    59341 ttttccttat aaacatccct attcctcttt tccctcttta tataaattta atttttaatt
    59401 agtttatcga actgtaaatt gacaatgcaa agtttagaat tgtattgttc gcattataat
    59461 atactgcttt caaataatat aaatcttgtt taatatcatt tgaacacagt atatctttat
    59521 aattatctta tgtttattat tttttatgga taccacattt aaaatattta ttaatttaat
    59581 ataaatattt tccgttatta ttaaaagtag ctatatttcg ttaatattgt atcactatcg
    59641 tgtagcaata acaataagta cattttatat attttttata atattattta atcatccaaa
    59701 cttttaccac tttttcaata tataaaatat tacactttag atgtaacaaa atcgcatcca
    59761 tttaaatatt gtattttata gactaatttt aggatgtgat actttggtta atttaaatgc
    59821 aaataatcta aatgtaaata tggtttatcc tatttcttcc acagcaccca tacgtttcag
    59881 aagatatact gtttcatatt tagaaaacat ggaaaatatt tatttaacta tagcaacata
    59941 ctttgataca cttggtttag gcacaatgaa tatcgacaaa gtttatggtc aatgggcttg
    60001 gtttactgat aatatatatg atttaaatct atttgttttt gtaggaaatt atccttatca
    60061 aattgctaaa aacagatatg atacatttgt agatattttg ccagtagcta ttacttctat
    60121 tgtcaatgga gatagaatat ttttaatttc aaatccttgt ttgttaaatt caaaaattac
    60181 agttagattt atatctagtc atcctactct taatagaaca gttgattatg gcaatattag
    60241 agattttatt ttttaaaact gcaacatgat gtgctattaa aaaataccct aaatttaatg
    60301 atattattaa ataactagaa tatttttaat tggcacatca tgcctagcta ttataaattc
    60361 ataatattat tttacttaag ataagtactg tattttatct acttgtatat ttttatttac
    60421 ctagtttctc agttctatat cctatttctt tattagctag cttataacct tcttgtatca
    60481 tttcttctct cgttaatgca ctccctaata aattgtcaat actcagttgc tttctatcac
    60541 taaaatactt atccaataat gattcctcta tatcttcctg atatgccaaa tttccttcat
    60601 gcattatata ttttacatca aaaccttctt tatttaatac ttttcccacc aagatacttc
    60661 tatgacatct tataggttct tgcatagctc ctaataaaac tatcctataa cccatttgac
    60721 aacccttttt taatctttct attccgttta aaaaaatatc ttcttttgct actttctcaa
    60781 aatctgcata accttcttgc gtatatacat ctttattagt tcttttagct ccaaattctt
    60841 ttcccatgta tatatataaa aatccggaat ttctaagaga ttcgttaaat gcctctttat
    60901 tgtattgaat attatattta gaatatggag tttctctaat gtcaactaca caatttatgt
    60961 catatttttt tatcatatta ataagcttat catatggata atttgaatgt cctattgcga
    61021 atatttccat ataaacaccc tcctattctt tgaaattagt atctatatat gtagataccc
    61081 gatttactta aaattaaata aagtattact ttaaataccc aatatctttt tttcttgata
    61141 ttgtatattt ttttacttta tatccttaaa aaaatataca actttatata taaaacatca
    61201 atatttatat atattttgta tattaaatac aattatttat aaagtcttta ataaaaattt
    61261 ataaatagag catctttaga aaactattat aataaaaaaa taagaggctg tctaaaaata
    61321 tagataagcc ttttttcata atcgtaatta taaaaatata cttgatttca tatgaaaaag
    61381 agtgtctcat aaactaaaat gtttactttg agacaccacc ttttttattc ttatttatac
    61441 tttgtagaca tattataaca tgcgtttcac tggtatctga acttctgtca accagtcttc
    61501 ttcactctct ttattccata taccatctat ataagattct ctaggatttc caaccacttt
    61561 atatttattg tcctctatcc atttaaatgc atctgcgtaa gtttttccta atgttgaata
    61621 tggaccattg tgtaatatac aaactgcatt ttcaactgca tctaattctt taaattttaa
    61681 tatatccgta tcttccttta aatcatttat agcttgacac atttcaacat ccatatcagt
    61741 ctctctatat tcaccatcat gatatatatt aaaacaataa tcaggaactg cacatgtaca
    61801 ccctgttttc atcatttctt cagccattac atttggaaat agattaaaaa gttcatcata
    61861 cccctttaac acttttctca tagaagcaac tttaactttt ggtaaagact taataattac
    61921 atcattcata ttgacttctc ctttcaaatt cataaggtaa ttacgtagtt taattatttt
    61981 atattcttca cttttaatta catccaacat ttctttttct ttattcatta aaatattttc
    62041 aacttcttca ggattttcta cagctttttt tatctcagct aatgataaac ccatttgttt
    62101 taaagttatt atctgatgta atttaggtaa ttgctctgtt gtataatacc tatattttgt
    62161 aaattcatct acatattctg gttttaacaa ccctatatct tcataatacc ttaatgtctt
    62221 tgtagttatt ttatttattt tagaaaacat tcctatagaa aacatctcat cacctgcgca
    62281 ttagatttga ttaatttaag tttaaacttt ccccttaggt taaagtcaat acaaaataaa
    62341 ttataattgt attaattctt caattctatc tccactcaat accaaaatat cccctgtttt
    62401 tataatggtt gaacctttag gtattaaaac ctgttccttt ctttttatca taactattaa
    62461 tatatcgtct ggtatattag aatctattat actcttatta gcccatttat taccctcatc
    62521 aacatttact tcgataagtt tagagctaat ttctccatca taatcattaa atgtttttaa
    62581 tacaggtgta ttatcatcaa ctaaatccaa ctttctggct acagttggta tcaatgaacc
    62641 ttgtattcct actgaaagca acgctataaa aaatattata tggaatatgt cattctctat
    62701 ggctactcct tgagtaacag cataaattgc aaaaactact gaagccgcac ctctaagacc
    62761 tacccacgat atgaataatt tttctttatt tgagaattta aattttgcta gaactataaa
    62821 tgttgctaat ggtcttgcaa taactatcat gaatatagat atggccattg ctataccaaa
    62881 tacatttggt aacttagaag ggaaagataa caatcctaac ataaagaata acattatttg
    62941 cataatccat gaaattccat caaagaaatg aaccaaactt tttttaagag gtatcctact
    63001 atttcctata ataatacctg aaatgtaaac acttaaatat ccatttcctc caagatactc
    63061 gctaaatgca taagaaagta ttgcaatagc tgttataaat atagtataaa atccttctat
    63121 ctcgaagttt gaatgtctta aaatataaac agataatttt gctagtataa atcctatagc
    63181 taacccaaaa gctatttgtt ttattaccat tggtgcaata agttctaccc cagaattatt
    63241 catcaatgtc aaaagaatta gtgtcaacat atacgctatt gggtcattac ttccactttc
    63301 aacttctaat aatgatgcaa gtgaaccctt taagtttagt ttttgagaac gcaacacagc
    63361 aaacacagat gcagcatctg ttgaagctgc aattgcccca attaataaac cttcaagtaa
    63421 actagttcct attaccatgt ggcaaaacat acctgtaacc ccagccgtaa ttattacacc
    63481 cagtgaagac attaatatag aaggtatagc aacaggttta gccatattcc agtttgttcc
    63541 aaaccctcca aaaaacatta taaatattag tccaatagaa cataattgtt ttgtcagttc
    63601 atagttatca aaatagattc ctaccatccc atcagaccca aataacatcc ctagtaagat
    63661 aaaaatcaat aatattggta ctccaaattt atacaatacc ttgctagaag ttatacaaat
    63721 caatagtact aacccactta taatcattaa ttctgtcata gcatcaactc catttcatat
    63781 aaattttgta gattaattta catctacaat tttattactt attcaaatat aacatgacat
    63841 aaattttata agttatggta cataaagttc aacaaaaatt tcacaataca ttaaaatgtg
    63901 agtgttatac ttaaatttat atatttgttt taattatatt catttaatat ttataatttg
    63961 aatttttttt tattaatatt tcatt
//
LOCUS       DAMJWU010000009        56808 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMJWU010000009 DAMJWU010000000
VERSION     DAMJWU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.009"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            276..2510
                     /locus_tag="PC460_000759"
     CDS             276..2510
                     /locus_tag="PC460_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2599659.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2541..3374
                     /locus_tag="PC460_000760"
     CDS             2541..3374
                     /locus_tag="PC460_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2599660.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKKKFDKND
                     Y"
     gene            3614..5347
                     /locus_tag="PC460_000761"
     CDS             3614..5347
                     /locus_tag="PC460_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2599661.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5739..6297
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5808..5836
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6827..6931)
                     /locus_tag="PC460_000762"
     CDS             complement(6827..6931)
                     /locus_tag="PC460_000762"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2599662.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7556..7801
                     /locus_tag="PC460_000763"
     CDS             7556..7801
                     /locus_tag="PC460_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599663.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIVFANCIMFSNILLEIFLIYICRKFRKI"
     gene            8796..9445
                     /locus_tag="PC460_000764"
                     /pseudo
     CDS             8796..9445
                     /locus_tag="PC460_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            9986..11341
                     /locus_tag="PC460_000765"
     CDS             9986..11341
                     /locus_tag="PC460_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2599664.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11459..12127)
                     /locus_tag="PC460_000766"
     CDS             complement(11459..12127)
                     /locus_tag="PC460_000766"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF2599665.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12194..12622)
                     /locus_tag="PC460_000767"
     CDS             complement(12194..12622)
                     /locus_tag="PC460_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2599666.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12729..13322
                     /locus_tag="PC460_000768"
     CDS             12729..13322
                     /locus_tag="PC460_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF2599667.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13441..14376
                     /locus_tag="PC460_000769"
     CDS             13441..14376
                     /locus_tag="PC460_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF2599668.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(14647..15591)
                     /locus_tag="PC460_000770"
     CDS             complement(14647..15591)
                     /locus_tag="PC460_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2599669.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            15909..17339
                     /locus_tag="PC460_000771"
     CDS             15909..17339
                     /locus_tag="PC460_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2599670.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            17539..18123
                     /locus_tag="PC460_000772"
     CDS             17539..18123
                     /locus_tag="PC460_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF2599671.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(18227..19006)
                     /locus_tag="PC460_000773"
     CDS             complement(18227..19006)
                     /locus_tag="PC460_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2599672.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(19064..20320)
                     /locus_tag="PC460_000774"
     CDS             complement(19064..20320)
                     /locus_tag="PC460_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF2599673.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            20578..21477
                     /locus_tag="PC460_000775"
     CDS             20578..21477
                     /locus_tag="PC460_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF2599674.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            21501..22949
                     /gene="ddlR"
                     /locus_tag="PC460_000776"
     CDS             21501..22949
                     /gene="ddlR"
                     /locus_tag="PC460_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF2599675.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            23008..23814
                     /locus_tag="PC460_000777"
     CDS             23008..23814
                     /locus_tag="PC460_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2599676.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            23840..25759
                     /locus_tag="PC460_000778"
     CDS             23840..25759
                     /locus_tag="PC460_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2599677.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(26001..26348)
                     /locus_tag="PC460_000779"
     CDS             complement(26001..26348)
                     /locus_tag="PC460_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF2599678.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(26335..28263)
                     /locus_tag="PC460_000780"
     CDS             complement(26335..28263)
                     /locus_tag="PC460_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF2599679.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(28674..30863)
                     /locus_tag="PC460_000781"
     CDS             complement(28674..30863)
                     /locus_tag="PC460_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF2599680.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            31194..31925
                     /locus_tag="PC460_000782"
     CDS             31194..31925
                     /locus_tag="PC460_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2599681.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEGDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            32005..32640
                     /locus_tag="PC460_000783"
     CDS             32005..32640
                     /locus_tag="PC460_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2599682.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(32891..33484)
                     /locus_tag="PC460_000784"
     CDS             complement(32891..33484)
                     /locus_tag="PC460_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF2599683.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(33555..34418)
                     /locus_tag="PC460_000785"
     CDS             complement(33555..34418)
                     /locus_tag="PC460_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF2599684.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            34581..35750
                     /locus_tag="PC460_000786"
     CDS             34581..35750
                     /locus_tag="PC460_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2599685.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            35851..36327
                     /locus_tag="PC460_000787"
     CDS             35851..36327
                     /locus_tag="PC460_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2599686.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            36893..37687
                     /locus_tag="PC460_000788"
     CDS             36893..37687
                     /locus_tag="PC460_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF2599687.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            37711..38154
                     /locus_tag="PC460_000789"
     CDS             37711..38154
                     /locus_tag="PC460_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF2599688.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(38243..39529)
                     /locus_tag="PC460_000790"
     CDS             complement(38243..39529)
                     /locus_tag="PC460_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2599689.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(39747..40637)
                     /locus_tag="PC460_000791"
     CDS             complement(39747..40637)
                     /locus_tag="PC460_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2599690.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            40929..41273
                     /locus_tag="PC460_000792"
     CDS             40929..41273
                     /locus_tag="PC460_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2599691.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            complement(41432..43528)
                     /locus_tag="PC460_000793"
     CDS             complement(41432..43528)
                     /locus_tag="PC460_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF2599692.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(43541..44701)
                     /locus_tag="PC460_000794"
     CDS             complement(43541..44701)
                     /locus_tag="PC460_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2599693.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            complement(44720..45787)
                     /locus_tag="PC460_000795"
     CDS             complement(44720..45787)
                     /locus_tag="PC460_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF2599694.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            46139..46705
                     /locus_tag="PC460_000796"
     CDS             46139..46705
                     /locus_tag="PC460_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599695.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(46900..47886)
                     /locus_tag="PC460_000797"
     CDS             complement(46900..47886)
                     /locus_tag="PC460_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF2599696.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(47916..48611)
                     /gene="pxpB"
                     /locus_tag="PC460_000798"
     CDS             complement(47916..48611)
                     /gene="pxpB"
                     /locus_tag="PC460_000798"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF2599697.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(48631..49851)
                     /locus_tag="PC460_000799"
     CDS             complement(48631..49851)
                     /locus_tag="PC460_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF2599698.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(49880..50671)
                     /locus_tag="PC460_000800"
     CDS             complement(49880..50671)
                     /locus_tag="PC460_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF2599699.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            51259..51888
                     /locus_tag="PC460_000801"
     CDS             51259..51888
                     /locus_tag="PC460_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2599700.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRSVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(52002..53516)
                     /gene="glpK"
                     /locus_tag="PC460_000802"
     CDS             complement(52002..53516)
                     /gene="glpK"
                     /locus_tag="PC460_000802"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2599701.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            53760..54302
                     /locus_tag="PC460_000803"
     CDS             53760..54302
                     /locus_tag="PC460_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2599702.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            54316..56808
                     /locus_tag="PC460_000804"
     CDS             54316..56808
                     /locus_tag="PC460_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2599703.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
BASE COUNT        20520 a         8566 c         7071 g        20651 t
ORIGIN      
        1 caatctatct atttatgaaa aaatatgata taattgcatt caaaaaggaa atctttcaat
       61 atactagaat ttaagcatta actaatttta tctaatatta ttactaaact aaaaaatatt
      121 tgtatttgta gcattggagc aagtaactta atgtatattc attttaaata cttatttaaa
      181 atttagcatt taaattattt acaaattaac atttaaatac ttctagcata aatacagttg
      241 tcattaaaat ttaaagtatc ttatggagga ataccatgca aaatcataat tatataaaaa
      301 tgttaaataa aaaggtagaa gtattatcta aggaattaga tagtagaaat tttaaattaa
      361 aaaccttaaa tgaaatagat tttttttcta aaataccaaa cagagaattt atgtaccaac
      421 atattgatga ctgtatttcg aaaaatatac atataggaat catgttatta aatatcgata
      481 actttaaatt atttaatgat atatatggat acctagtcgg agaaaattta ttaattactc
      541 taactcaaaa cctattgcac tttattccag aatcaaaggg aatagtcgct tatttaaaag
      601 gagaaggttt tttaattgta cttccaaatg tatcaaataa agagttaaat aatatgggtg
      661 caactattat agagagcgcc cgagaattaa aaataaaact tcctaatgaa aaagaaatat
      721 actccaatgt tacatttagt atcggcagca ctatctggaa tggtgaacca aacatgacta
      781 ctttgatgct tttcaaccaa gtatatagtg ctttaaaaaa ggctaaaagt gaaggtaaaa
      841 atagatacat tctatttgat tttaaagata atagttttta caataaatta gaaaatgata
      901 aggagttctt atcagaaata tcacatgttc tacaaactaa acaaattgaa gtattttacc
      961 aaccacttta taatataaaa aataatacta tagttggatg tgaggcactt gcaagatgga
     1021 gacaccctat taaaggactt cttactccag ataaatttat tcctttattt gaacagtttg
     1081 gtctgataac ggaattagat ttgcatttat ttgaagagaa ctgtaaaaat ataagaagat
     1141 ggattgataa caatgatgtt tttgttccaa tttgttgtaa cttttctcgt cttcattttt
     1201 ttaaccctaa ttttccaagt atgctaaaag gtattactga aaagtacagc gtctctacat
     1261 caaattttag cattgaaata acagaaaata tattaataga aaatacaaat acaattattc
     1321 aacaactagc tgaacttcgt tcttatggtt tttctgtatc aatggacgat tttggttctg
     1381 gatattcatc ttttggtatg ttgcaaaatc taccttttga tataatcaaa attgatagga
     1441 tatttttatc tcataattta aatgatttta aaaatacaac cattttgtac tctataataa
     1501 acattgcaag agctcttggg atgcttacag tatgtgaagg tgttgaaaca gaacaacatg
     1561 tagaatttat taaaagtata aattgtgata tcgcccaagg ttattattat gcaaaaccca
     1621 tggagtgcaa agaatttgaa aaattactat ctaaagagaa taattcagag aaaatgttta
     1681 taaaatattc tgatatagga aaatctttaa tagagaagat atttgatgga ttttttatta
     1741 tgcaagatat gaaaaaacta agagctaatg tcactgaaga cattcaatgg tttgattttt
     1801 acaacaaaac tgaaattaca tcttttccta aagtaataga gcattttgaa aaacatatta
     1861 aaggtaaaaa attttctatt ctttacaaaa gtataacccc acataatact gaatcagatt
     1921 cattattaat tagtggagaa gccgttttat cagatgaagc ttctgaacat caaaaatata
     1981 gcaatttcta ctttagtgca aattgtataa tttcagaaaa taaattactc ttagcaaaat
     2041 tacatttgga tttaattaaa actgctggct atataaatga atttgaatca tattcaatac
     2101 ctaaaatgac aaatacacat cttgatgaaa gtaatatttt atcacaattt tattcaatgt
     2161 tgcctgtagg tataattcgc tacgatttat ttggtgatat gattattact tatatgaatg
     2221 aagaaatgtt tgatattata ggttatacaa aagaacagtt ttttggtgag atgggcggaa
     2281 atttaagagc aattgttcat ccagatgatt tagactttgt ttataaaaaa agtttggata
     2341 tgattgacac aaataaaatt gaaccatttt tatatagatt cataaaacga gatggtcaaa
     2401 ttgtcacagt tatgtataca caatgtaacc tttctgcaat tgatggaagg tcgattgccc
     2461 agggtatgta tgtagatgta aataaaataa aaaatttaac aaaaatctag tacaaagtat
     2521 tttattttgg agaaattaat atgtttaaag aaattttttt aagaactttt ccaggatttc
     2581 taattataag agattctaac tacagaatta tatttataaa tgataattta aaaaatttaa
     2641 ttaaaagtta tatgcaagat aatccacttg gaatgactaa tatagaaata gctaagaaac
     2701 tccctgataa tattgctaaa ttttttacgg attctcacaa tattcaatta gattgggaaa
     2761 aaaattatcc ttatgataaa atttcaaatt ggattttaga gtttaaaaaa gatactacaa
     2821 gctattggaa tgttttagaa tataaagtag atgttgatga aaaaacctac ataattacta
     2881 tggccaatga tataacaaaa ctctatgaag aaaataagag aaatcttcac tactcaatta
     2941 ctgacccatt aactggagca tataatagaa aatatttaaa tgatagattt gacatattta
     3001 taggagatta tattgtacta attgacttag ataatttcaa aatgataaat gactatgaag
     3061 gtcataatgt aggagacaag attttatgtg attttgtttc acttttaaat aaagaattaa
     3121 taaattctac ttctattata agattaggag gagatgaatt tattgttata ttctctagtg
     3181 atgttgacaa aaattatgtt tattcacaac tagaagcctt aagagaaaat tttttaaaag
     3241 tattctctaa gtataagtat ctttcattta gttatggtgt tgatacagta aaaagaaatt
     3301 taaaattgac aatagttgaa ttggataaaa aaatgtataa aaacaagaaa aaatttgata
     3361 aaaatgatta ttaatattgt tattttaaaa taaattaaaa ttatctttct ttttttaaac
     3421 ttgtacatta gtttataaat atgtatctat acaagatatt gacattaagt actgaagcat
     3481 atattgtttc agttcttttt tgttaaaatt aacttattta acaaagatat catagtttaa
     3541 caaagctttt aatttaaata tattgatgta taatataatt aaaatataat agcaaaaaga
     3601 aaggaggaat atcatgagca tactcctaaa aaaagcaccc aaattagcaa aacatattat
     3661 aacaagtttt tatataaatc gggatattga tgaagtatta aaataccttt gtgaaaatgt
     3721 tacatggatc ggaccaggag aacaggaatt tctcacttct tttaatgaaa taaaaaatta
     3781 tttttatgca ggtcaagatg aaattccttc ttgtgatata aataatgata tatttgaaac
     3841 tgtaagtgaa gatgagaata gatgtatggt attaggaaga tatactgttc gaactaaaga
     3901 aaatactcaa atgattttag aggtaaatca acgttgtaca tttgagataa ttgaggatag
     3961 aggaaaatta ttagtaaagc acatgcacat atcaaaccct tatggtgaaa tgcaacttga
     4021 tgaatacttt cccactaaaa ttggaactca aagttatgat tatttgcaaa gattactaaa
     4081 ggaaaaaaca gaagttattg aaatgattac aaataatatc aatggtggct taaaaggtag
     4141 caatgatgat aatacatact catttttcta tgtcaatgaa ggattgccca aaatattagg
     4201 atacacatat gatgagttca tggaaatgag tggtggttca gcagtgggtg ctgtatatcc
     4261 accagactta cctaaagcac tagaagattg tgaacgatgt tttgcaaaag gacctacata
     4321 ttcatctgaa tatagaatcc gaaaaaaaga tggaacatta atgtgggtat tagattctgg
     4381 aatgaaatca ttaaatagtg atggcatagt aaaaattaat agtattatta ctgacataac
     4441 ccaactaaaa aacatagaat ctgaactaaa attagaaaga gagcgatatc gtattgcttt
     4501 gcaaaatatt acagatatca tgtttgagta tgatattgaa aacgataccc ttataaaata
     4561 tcaacgtgta gaaattgaca aaaaaattga attagaaaat tttgaaacaa aaaattactc
     4621 caaactatta gaatctggta aaattattca ccttgatgat attggaaaac tactagaagt
     4681 acttcatgga aatcttcgtg aaacaattga gataagacaa gtaaattcat taactaaaaa
     4741 tgaatggaga tggattcgtg ttcaatgttc agttatatat gatagtgacc ataaccctat
     4801 caaaacaata ggcgttttaa aagacataac tgaagataaa tctaaattag aaagcttaat
     4861 taatcaggct caacgtgacc cactaacaca attatataat caaagagtat cacaaaatct
     4921 tattgaagag tatctatgta gttctgattc caaaaataat gatgctctat tagttattga
     4981 tattgatgat tttaaaactg taaatgatac ttttgggcat ttagagggaa atgaagtttt
     5041 agttgctgta tcaaaaatac tattacataa cacacatgat aaagacatag ttgctcgaat
     5101 tggtggagat gaatttacaa tatttataaa atcattaaca aaggatttaa taataaaaat
     5161 tactaatgat attttaaatg atgcaagtaa aataaaagta aaggacaatc ataaaataac
     5221 attaagtatt ggtattgcct tcactgatga ttcaactaaa ttatataaag atttattttc
     5281 taaagctgat aaagctcttt atctatcaaa agcagatggc aaaaattgtt atagtacata
     5341 cgaataataa agctatttat acgaccaaga atgaaccttt aaatttaatt tcatagtcta
     5401 acaatcaaag agttgaagct aatcttatag ctagtcaact agttcctaga aaaaatttaa
     5461 acaatatatt tattgatact gctcattttt ctaaagagca aatttatata aaaaattaaa
     5521 cttttatgta catacagata ctaatgataa acagttatca ttagtatctt ctatcttaaa
     5581 acaaaatgat aatttatcta tataaaaaag tgctttgttg acagagttca cttttttata
     5641 taggtacaga atttaagtat aaatacatgt tttattctat acattttagt taatgtaaaa
     5701 tttcttgatt gtactctcta acttttcaac attatactat ttatatacca cttagttaat
     5761 ataaacccct tagctggttc tagtgctttt tctatgattt tagtcatatt tacataccac
     5821 ttagttaata taaaaccaat ttagatgatg tatatgtgca aaaggaagaa ataatttaca
     5881 taccacttag ttaatataaa acgctactaa acttgatata catgaccatt ataataataa
     5941 atttacatac cacttagtta atataaaact tccataaaaa catccccttt ttattcttct
     6001 tccttatatt tacataccac ttagttaata taaaacagtc tttttttaat gcagagatgc
     6061 aagaagtttt ataatttaca taccacttac ttaatataaa actatcagta tttaagtaat
     6121 aatttaagta atataggatt tacataccac ttagttaata taaaacggtc actacaactt
     6181 atacttccag tctaacgatt tgaatttaca taccacttag ttaatataaa acatattttg
     6241 gatttattaa tataaacata attaaaaaat ttacatacca cttagttaat ataaaacccc
     6301 aaaataaact gggcattttc aatacttaca catatgcaac tctcttaaat ttgcagtgaa
     6361 ccatgagtag tgcaaatgat aaagcttatc atataccctc aacaccttat atttcaagcg
     6421 ttaagctcta ctttgtaaca aatatcgctc actgcaaaac tatttacatt tttattatat
     6481 cataaatata ttatttttga atatctatac caatttgtgg tataataaaa gcaagaagaa
     6541 ctacaatcta tttagacgta gagtgaagtt caaatactaa tgtttattct ttttgaactt
     6601 gatttttagt tcaaaactaa agtcactcct gcctgtgtga actatacccg ctgttttata
     6661 cagttaaaac atgtaaaaat agttataaag tactagtgta actcattagt actcttttta
     6721 ttatattcat ataattaata tctatatttt aaagtttact atctaaaaaa gagcacgtca
     6781 aataaaaccc tagatattta atttttttag agatacttaa aataaattat gctctctctg
     6841 tacttaaata tttcctaaat tcaaatggag acatagaatt taatctattt tgatatctaa
     6901 aattattata gtagtatata taatcatcta ttgctttaac taattattca tatgtatcaa
     6961 atttccttaa atagtacatt tctaccttta aaattcccca aaatccttcc ataggagcat
     7021 tgtcaaggca acgaccaact cttgtataat atatgtatat ctaacaccat taagaaatat
     7081 atattttata atatatattt cttcatttaa tgcacttaga aaagcaaaga aaatacagat
     7141 tagtacactt tttagagaaa aataaaatta atacatctaa aatattaaaa aatactttag
     7201 ataatctaaa gacacatctc aaagttttta actaaattat aaaactaatt acaaatgcta
     7261 tgtaacgata ataaataaaa aatttacttc gcttaagcga cgtgtcgttg aaatatttca
     7321 tgttgccaac tggttcgtaa gttgataaga aaagaacact aattatttag agttcttttc
     7381 ttagctacaa gtggattata atacttttaa atctgtgaga aaatcatata taataatgat
     7441 atgaacaagt aaagaaaata caaaaatgac aaaattataa ataaaatatt ataaaatcat
     7501 aaggtcataa ttgttatatt gtagaaacaa agtaaagaga gagggtggag aataaatgaa
     7561 tcaaaaaaaa ataaagatta gtattaaaat ggtaattata ggtatatttg ctgtaattat
     7621 tgcttttgta ttatccagat tattcgttga gtatattgat gaattattaa ggtctaattg
     7681 gcaatatcag ctatttgaaa gtgagagttc atatatagta tttgccaatt gtattatgtt
     7741 ctcgaatata ttattagaaa tatttttaat atatatttgt agaaaattta gaaaaatcta
     7801 atatggtcaa cgtatttcga atcgaatact tcatgattat ttagtctatt gtgctagtta
     7861 aagtttaatt cccatataaa gtaaattgat tttagtaaaa caaatcaccc ctaagggtta
     7921 gtttatggat aagtcatcca aatattaact ctttaatttt tgctatagat tcatctttgc
     7981 tcataacttt acttataaaa aatgaaatat tataagctag aacttttatt ctaattctag
     8041 tcataaatcc tagcatagag ttactttttt tatattataa ataaaactat acaacaaaga
     8101 taatatttag taaaaataac tttcatttta actaaactta aaattcaatt actctctaaa
     8161 ttgacgttta ggaattagtt caaaataaaa aagagtgtat atattgaaat tactacattt
     8221 caatatatac actcttttta aatttctcag ttttccatgg aactttactt tatttatttc
     8281 tgtattagtt gtatttttta tgcatttttc tttaaaatca tattttctga ttcaactctt
     8341 atattataaa atttctttac taaattattc tctttttcta tatcttcata tatatttttt
     8401 aatcgccttt tacctaatct tttatctaac attactaatg atttaactaa tatattatat
     8461 gattttatcg catcttctat tgagatattt aaacatatat ttactgcttc ataaaaatca
     8521 aattcattaa aaaatccatt tttatttaca atcttatatc cctttgcatc tctttctaaa
     8581 tactcttcct aaatactctt caaaactttc atcatctaat ctaaatccta tatcactatg
     8641 aaaaataggt tttgcatttg gatatagttc tattgtttta tcaaaagttt cgaataccaa
     8701 gttattatta ttcgattttc caacagtcca ctataaacaa taataagttt gttgctccaa
     8761 ataatgtaac acttgtagaa agatgttaca gatatataaa agatagagaa tgtagttttt
     8821 ctcctaatac tattagaaat agaaaaaata tgattaaaaa tcaaattgaa gttttcttta
     8881 aagatatgaa attaatagat ataactcctt ctattttaca gtcttatata aataacatat
     8941 acaatgagca catgttaaat tcaacaaaaa accaagtcga ttttataaaa tcagtattga
     9001 aagaatctta tagattaaaa gaaatatcag aaaatatttg tgattttgtt acaaccccaa
     9061 tcaaaaaaaa tagttcaact tctaagcttt atacaaaaca aaaagctcaa ttattattag
     9121 aaaaatcaaa aaatattcct ataggtatac caattttttt gatgctaact cttggattac
     9181 gttttggtga agctgtatca cttatatggt cagatgtaga ccttgataaa aaatctcaca
     9241 tgtaaatcaa acattagttt atgtatacaa taaaataatc ttcaaaggcc ctaaaacccc
     9301 tagattcaaa agaagattat ttgttcctga tgaacttata agtttgttta aaggggaaaa
     9361 attaaaacaa aataagttaa aattacaggg tatattgaaa aatgaatttg atttgatatg
     9421 cttaaataaa agattcaacc cgtgagttca accaactttt gttgttcatt ttaaaaaact
     9481 attaagagat aataatccta gagatataaa agtacatgaa ttaatacatg caaatgcgac
     9541 tttgatatta ctttcaggta ctaacataaa agctatttat aaaatattag tacatgcaga
     9601 tataaaaata gctatgaata tatattccat attctcactt attagatgaa atgaataaag
     9661 aaacatctga aaatataagc aaaatactat tcaaataatg ttcagtttta tgtcaattaa
     9721 atgtcactca aagtgctttt tttccatttg tcagttaaat gtcacttaaa gtaccttttt
     9781 cctatttgtc agttaaatgt cagtaatata taaataagat atatttataa gacccttaaa
     9841 atcactatta tttaataaaa tttataatta tatactattt gctgttgaca ttggtattat
     9901 accatagttt atcatttata ttagaataaa attattaatt aatagtttcg taattttata
     9961 atagatttac agggagtgat aataaatgga tacaaaaata tctataaacg aaaacatgag
    10021 tctaggtaaa agatttttta aatacctagc accatctgta gttgcaatgt gggttttctc
    10081 actatataca atggttgatg gaatatttgt aagtaaagga gttggagaat tagctttagc
    10141 tgctgtcaat atatcaatgc cttttattaa ttttatattt gcagtttctc tattattttc
    10201 aactggtgca tctaccataa ttgcaatcta tctggggaaa aaagatatta aatctgctaa
    10261 cgaagtcttt tcatttaact tagtatcaat aattatatta tcaataatta tattagctat
    10321 aacttttttt aatttagata gattagcact atttttagga gctacagaat ctacaattgg
    10381 tatggttaaa gattatttag gaataataat attttttaac ggattcttta tagtgtctta
    10441 ttctttagaa gttataataa aaactgatgg ctttccaata ttagctactg ttggtgtaat
    10501 aatatctgct cttacaaaca tcatacttga ttacttgttt gtaattgaat ttggatgggg
    10561 tgtaaaaggt gctggtattg caactggttt gtctcaagtc ttttctacta ttttcttctt
    10621 aatacatttt ttaagaaaaa actctacttt gaatttttca aaatttagaa ttgattttaa
    10681 gacacttaga aaaattgtat ttataggttt tcctgattca actactgaat taagttgtgg
    10741 tatagttgtt ttacttttta acttaagtct tactaaatat attggagaaa atgctcttat
    10801 ttattatagt gtcataaatt atataaatac attagtactt atgactatga tgggtataac
    10861 tcaaggtatg caaccactta ctagctttta ttatggtgca ggaaatatag ataatgtaaa
    10921 aaaattatta aaaatgggga taaaagccac aataatagca tcagttgctg tatttgcaat
    10981 ttgtatggct ttttcaggac caatagtctc tttatttata catccagaag aaacaatgct
    11041 attcaatgaa ggagttcgtg tatttaaaat attctctata tctttcttat tggtaggaat
    11101 taatgtaata atttctggat tctttgtatc agtagaaaaa ccatcaattt ctactgttat
    11161 atctcttggt agagggcttg taatagttgt tttaagtcta atttcaatga tactaatatt
    11221 tggtggacaa ggaatatgga tgactactat tgtttctgag tttatatgtc ttattttatc
    11281 attagttttt ctaaaaaaga atttttctac attagactct aatctaaata aggttgctta
    11341 gatgataaga gtgacaatat atgaaaatat ctaaataaaa aatacctatt atacaatctt
    11401 gcaaatatta aacctttgca aaaattagga ttatataata ggtattttat ttatgtaatt
    11461 attttatttc taaatatcca tagccatctt ttattaaagt tccttttact tcatattcta
    11521 caggtttttt aaatattgga ccatctacga caaatgtatg atactctcca tgttctccac
    11581 atgggtctgc accagcattt tttatcttct ccacaagttc tctagttaat ttttttccta
    11641 aaaattcctc tcctaagtgc tttaagttta cttttgttac aacactacaa aatccactgt
    11701 ctataaattc atatactaaa tcctctctac tctcttgcca taatggtaaa tctactttca
    11761 taccaactgc ttcacatcta tcagtatccc attttctatg actttctata tctatatctc
    11821 catatgcaca tatcgttgct cccatgtttt tagcttttat taaagccctc tcaaagctct
    11881 cttcatatcc ttctacgcca cactcagcaa ctaataaagg taatcctatg ttgctactta
    11941 cttgcttaag catattatat tctaaattat gtgtccatga ctttccatgt tcttcactta
    12001 tagttgtcaa gagtgccaca ggcttatatc cttttttcaa cattcgattt aatgctaata
    12061 tactatcctt accaccacta aatgacatta caaatttttc atcagccaat tttaacacct
    12121 ctttcatcta actttgtttt attaacataa ttataccaaa tttttgagtt gcatagcaaa
    12181 ttatattaaa ttgttaattc actataaaga atgtccctaa tattaaaagt ataaccccta
    12241 tgtatttgta taaatgcatt gtttcttgtc ccaaaattac gctatcaata aaaaatccaa
    12301 ctaaaacttg ggatacaaga gttaatataa gtgtatttga tactcctaaa tttggaattg
    12361 cctttactgt aaaatatatt attagacctc caaatactcc tcctaataac aacttataat
    12421 taaaaccatt tatagatact agacacttta agtcacctgt aactattata cttgccaaaa
    12481 aaaatattgc tcctacagtt aagcttataa aggtggccac taatgctgtt gtactttttc
    12541 ctagctctcc atttataaaa gcttcaagag ctgtagaacc acctgctaat aatgcaaata
    12601 ttattgccca taattttgtc atggacaaac ctccttctat ataattctat taatagaagt
    12661 ttatgataca acctattaat taattactaa gtatagttta atttttatac actaggcttt
    12721 atttggatat gaatttctgt tataattgtt ctatcgattt tggaggtgat tttatgtaca
    12781 caaattttaa tatcgatttt aataacttta ataaaaaaga aaatgctatt aaatttatgt
    12841 taatgggagt attactcatt atattaggat tattatgcct tacatttaag acattaggta
    12901 taaaattaat ttcttggact tttggtattg cacttttatt ctttgcatat ttaaatttaa
    12961 aaaatattaa tgagttaaaa agatatgcta ctaaagaaga aattaaaccc tctattaata
    13021 tccaatgggt tctaataatt gcatgtatat tactttttgt atttcctcaa aaaatacaaa
    13081 gcatattttc attattttta ggattttatt taatctttaa tcaactagta gcacttgtaa
    13141 atagtaagaa taatccttat tctaaattta caacttggaa tatagttaag attctatttg
    13201 gtatatgcct tatattatct ccactttttt tatctagatt tatagtatcg ataatgtctt
    13261 ttttcataat attatttgga ctagttctat ttttctctgg aaatacagcg agaaaatatt
    13321 aattattttc tcgctgtatt tttatattac ttaaaataga atttgtttaa gttatctatt
    13381 tgtggtaata tgttttttgt acaagctcag cgtacaattt tattagaaat aggtgatttt
    13441 ttggataaat ttaaatatgc ttttgataat aaaagatatc atacttggaa ttactatctt
    13501 agaaatactt ttggagaaaa agtttttaag gtatctataa atgcaggatt tacttgccca
    13561 aacattgatg gaagtcttgg ttatggcgga tgtacctatt gtagcaaaga aggctcaggt
    13621 gattttgctg gaaaccctaa agataatctt atcagtcaat tttataatat aaaagaaatg
    13681 atgttaaaaa aatggcctca tgcaaagtat attggctact ttcaagccta tacaaatacg
    13741 tatgctcctc ttgaagtttt aaaagaaaaa tatgagacta tattagaact tgaagatgtc
    13801 ataggtcttt caatatccac aagacctgac tgtttgccag atgatgttgt agaatactta
    13861 tctgaactaa ataaaagaac caatttatgg gttgaacttg gacttcaaac aattcatgat
    13921 tctacatcta aaattataaa tagaggtcat gattataaaa catttgttga aggtgttgaa
    13981 aaactaaagt ctaaaaatat aaaggttgtc gtacacatta taaatggtct tcctggtgaa
    14041 gattataata tgatgatgga aacagcaaag gcagttggta aaatgggtgt agacggtata
    14101 aaaatccatc tattgcatgt tataaaagat actcctatgg aaaagatgtt acaaaatggt
    14161 atgttaactc ttatggaaca agatgaatat atcaatcttg tctgtgacca attagaaata
    14221 cttcctgaaa ctatgataat ccatagactc actggtgatg gtaagaggga tgaactagta
    14281 ggtcctatat ggagcctaaa aaaatgggaa attttaaatc aaattgatga tactttaaag
    14341 gctagaaatt cttatcaggg ctgtaagttt gtttaataca aaataataac tatagaaatg
    14401 gttatataaa tatagtcctt tctatagtta tttagtattt acaaaacaaa atattaattt
    14461 gtacatttat acaatatata ataaaaaata gatagtctaa actgtatcta cttatatttt
    14521 atttgtactg tccactttat tctataaaat ttataaatag ttatttttta ataccaaata
    14581 taatttggta taatttttgt tggactctaa aatgtatacc aataaatcct ttttaagtat
    14641 ataattctaa atactttcag ttttttcaat ctgctttaac ttgttggact tataaaatat
    14701 tcttatttca tcataagaaa gattaagtgc tactactgca gatattaaca tcaacatcac
    14761 tccaaataac ttccctaagg aaaaactttc tcctaatata gtgcaaccaa ttattactga
    14821 accaactaat tctactgatg ccacaacacc tgcaactgac aactctacac ctttagatat
    14881 acctgctgca taaaaaatat atgcaagtgc agctggaaca atgcctaaca ttagcataca
    14941 agataaaaca tctaaatcct tagcataatt taagatttcc caaggtcttg aagaaggaat
    15001 cataaatata gctccaaata agaaactgta tactaatatt gttgaacttg aaaattcctt
    15061 taaagcattt ttactaatag ttggcataag tgcatatgct attgctgata ataccccaag
    15121 taaaagtcca aaagcgttta aaccttgaaa atccaaacgt ccaccagtca ctgccataat
    15181 tgcaccaata aaacaaagaa taagtgaaaa taatttgctt ctagtaatat tttctttata
    15241 acatattttt gaaaatattg ctaaaaacag tggtgatgta tatagtaaaa ctgctgcaat
    15301 tgatacaccc gcaatgtcaa ttgctttaaa ataacataaa ttaaacattg cctgacatat
    15361 aatacctatt attactgaat atataattcc ttttttgctt atctttaaga tttgtggtgt
    15421 ccttatagat gaatatacgc ctaaaactat aaatccaaaa aacaatcttg taaatgctac
    15481 ttgttctgat gtcagtccat ttcccattaa aaactttcca aataatccaa gtgtagccca
    15541 taataaccct gctattgcta taaatacata ccctttactt ttttctccca ttttaaattc
    15601 ccttctattt ttatttagtc aatttacact cttcttttta agcaaaaact atgccatctt
    15661 aaatcaatat atatcgactc taaaacccta gtctttccaa taatactaat aaattttcaa
    15721 tttttttata tttatttttc acttacttta agttgattta gacttatttt aattgtaaaa
    15781 tgagttatat ataacttatt taaagttaaa atagactgaa acttatatat aaatatcaaa
    15841 ttttaagtat attaatcaat taaacctatt ttaatatatc attaaaaatt taggaggaaa
    15901 atataaaaat gaatttagat ttggaatttt atcaaaaaat attagaagct tcccatgatg
    15961 aaatatgtgt ttctgatgat aaaggaataa taatatattg taataaagca tttgaagaaa
    16021 attatggtct aaaaaaagaa gatattttag gaaaaaatgt ttctttttta gaagactctg
    16081 gatattcaac aaaaagcccc ataccaattg tattaaaaac aaagactaaa ttttcactgg
    16141 aacaagatac tcaaacaggg aaaaagctta tcataactgc tacccctata tttgatgaaa
    16201 atggaaactt agaatttact gttgaaaatt gtagagatat aactgaactt aataatataa
    16261 aaaataaact tgaagacaca aaaaagcaag taaaaaaata taaatctgaa gtagaaacac
    16321 tatacagaac tgcacttaga attgaggata ctgtaattat ggacggaata gttatgagac
    16381 caatcataaa cacagtaaat catgtttcta aaacggatgt aagtgtactt ttattgggtg
    16441 aatctggaac aggaaaaagc tcacttgcta gatatataca ccacaacagc aatagatcta
    16501 atggcccatt tataacaata aattgtgcaa ctatatctcc acaacttctt gaatctgaac
    16561 tttttggata tacttcaggt gcttttacag gtgcaagtac taaaggtaaa gttgggctcg
    16621 tagaattagc taatggagga actctttttt tagatgaaat aggagatata ccacaaaatc
    16681 tacaggcaaa atttttgcaa ttaatacagg atagaacttt tactccagtt gggagcttaa
    16741 aaaataaaaa ggtcgatata agaattatat ctgctacaaa tgttgattta gtttcaaaag
    16801 taaaagaaaa aaagtttaga gaagatttat actatagatt aaatgttata gaaataaaat
    16861 tgcctcctct tagagaaaga agggataatc tagtggaaat tataaaatat tattttaata
    16921 ggtactcaag tgacttcaat ctaaataaaa ctatttctaa agaagctatg gatgctattg
    16981 caaattatag attcccaggc aatataagag aattacaaaa tataattcaa aaaatcttgc
    17041 ttacttgtac agataatcat ataacaatag atgacttacc aaacattctt acaaaaaata
    17101 tacatattac caataatgga aataaaacac atatatccca aattaataag gttattatat
    17161 ctgactcaaa atctactaat tataaaaata aaaattttga cactttaatt aaagagtatg
    17221 aaaaaaatat aattttggat gcttatgaaa aatttggtag ttcttataaa gtagccaaac
    17281 accttgaaat ctcacagtct aaagccaata ggttaattag aaagtatact aatacttaat
    17341 tttaaatttg ataaataaaa acttaacact atttacatat tgtaaatttg gccatttact
    17401 gttattatga atatggttga aattatttaa ataaatactg ttgacttagt ttcatatatt
    17461 taccattaaa cttttatcat aaatatcata tcataataat atatttaata agtctaataa
    17521 actatacgag gtgtaaatat gttaatgaat acccatttta ttatggcaaa atcaatactt
    17581 gataatatag atgaaaataa aacattcttt ataagcgaaa aaaattttat atatggcaat
    17641 ataaagcctg atgctttttc taaatataaa ttaaaaaaac attatttaga tgagtcattt
    17701 aatatgattg tttctaaaat aaactatcta tgcagtctta atatagatag tctttctaaa
    17761 tacttttcag taagtagact tagtcaggaa ctaggagtca tatgtcattt tttatgtgat
    17821 tttttctgtg tagctcacag tgaacgttgg gagtttaagc atagcttaaa taaacatgtt
    17881 acttatgaaa gagaacttgg agcatttgca aaagatgttg atttatctaa aattaaatgt
    17941 ggtactataa atagtagttt tgagaagttt tttactaaac tttatagcga ttacaaaaag
    18001 aaaactgatt atatgaatga tttaaaattt tcagcttata cttgtaatag tgtcataaat
    18061 tatatacttg actctatttt atctaataca ataaaatcat atcatatagc aaattgcggt
    18121 taacttaaga atttttaaac ttatataaac ttataattga atataattaa aatgactgct
    18181 ctatagaata taattttata cttctctagg acagtcattt tttatactaa ctatttatct
    18241 ccattataac atctatggca ctttttacac tatctactac ataatttgca ctttgcttta
    18301 catcttccct tgccttagac attgcaaaac tatatttaaa tccttctaac atgcaaatat
    18361 catttggaga atccccaata gtagcaactt catctggact tacatttaac gtttttatta
    18421 catgctctat acttcctctt tttgacacac ttttaggaac tatatttaaa gtatatgttc
    18481 cagaaaaata tatttctaag acttcactaa actcactttt tagataaagt tctattctgg
    18541 ataactcttc caaattttca gaacatacgc ctatatttcc tataagatta tcaaaactac
    18601 ttcttttttc aaagtctggg tcaactataa agtcactaga ataatgtcta aaagcagtgg
    18661 tatcactacc cacaattaca tcacccttat atttaaaata aagttgtgca tcacttgact
    18721 caaatctatc aatgactttt tttactaaat tatcatctat cgttctttta aaaattacat
    18781 tataatcttt atcatatata aatgaaccat tttcacatac atagtatcca ttcatatcta
    18841 aactatcttt gataatatta tgtgcttgtt taaaaattct acctgtagca acattaaact
    18901 gtactccctt tttttctatt tcttcaattg ctactatatc ttcttttgtt atggttccat
    18961 tttcatatag agttccatct aaatcacaaa atatatgttt tatcataaat taaaagcctc
    19021 caaaaacaat tttatatttt actaatactt taatcaaaat aacttaaagc ttttctaatt
    19081 gtattctcaa ctcattaagc aaatctctag tagctcttaa tcttatcttt cttctatccc
    19141 cattaaatat atttttcttt actattgttt ttccatttat atatattgca gtataaacca
    19201 gtcccacagg cttctctttg gttcctccac caggtccagc tactccagtt gtagatatac
    19261 ctatattagc tttaagtcct ttagcaacgc cctcagccat ttctatagct gtttgctcac
    19321 ttactgctcc aaactcttcc aaagtttctc tttttactcc taatcttttc attttagaat
    19381 cattagaata tgttacacaa ccttcaagaa atacagagga tatacctgga tagtttataa
    19441 gagatgaaga aaccaaacct ccagtacaag attcagccac tgctattgtt agattttttt
    19501 ctacaagtag cttagaaaca gcatcttcta aagcagtctc cccttcagat atggaaatat
    19561 ctccttcagt atatacatat ttaccaactc tactttttat tttttcttct actggtttta
    19621 ttaatttaaa agcttctctt tcattttcag cctttgcagt tattcttatt gtaacttcta
    19681 attcttttgc atataaagca actgttggat tagtctgctc attaattata tcgagaattt
    19741 ctaattctag atttgactca cctattccat ataatcttaa agtcttagat actagcattt
    19801 catttgtgaa ttgttgtaag taaggtttga cactctcatt aaacatagct ttcatttctc
    19861 ttggaggtcc aggcaatact attataaatt taccatcttt ttttaaaatt gcccctggag
    19921 ctgtaccatt attatttttt agtactatag catcttttgg aaagtacact tgtttcatat
    19981 tattttcggc catttttttc cctgttttaa caaagaaaga ttccaaaacc tctagtgaag
    20041 gtttatgtag ttctaatttc tgaccaaagt attctgcagc agtttctttt gtcatatcat
    20101 caccagttgg cccaagacca cctgtagtaa ttacaaaatc acttctcttt aagctttcat
    20161 caaaacattc taaaagtctt tgtttgttat ctccaacagt actctgatgg tatacttcta
    20221 ttcccaatga tgcaagttct ttagctaaaa attgagaatt tgtatttact atatctccca
    20281 ataatatttc tgttcctaca cttattattt cagctttcaa aatttatcac tccttgattt
    20341 atagccatta agactagtct taagttcata acaataattt tagcaaaaat aataacataa
    20401 ataaagttaa aaaatcattt aaacatatct ttaaactaga ttattattct aaaaccacac
    20461 ataatataaa cttcgtatat agtggtactt tcatcctcaa ctgttaattc atataattaa
    20521 gtatgtaaat aaaaagttta ctttaaaata ataaaaatta aaataggagt gtataatatg
    20581 aaaatagcag ttataatggg tggaatatca tcagagagag aagtttcttt aaattcagga
    20641 aaagagatat ataataacct tgataaaaat aaatatgaag ttgtaaaagt aataatagat
    20701 gataaaaaag atatatttac aaagatacca gaagatatag attttgcaat acttgcttta
    20761 catggaaaat ttggtgaaga tggatgtata caatcaatac ttgagactat ggatatacca
    20821 tactctggat gtggacctct ttgcagtggg atgtgtatgg ataaaaatat aactaaaaaa
    20881 atgcttagag attcaaacct acctactgct ccatgggttt tagtaaaatc agttgatgaa
    20941 atagactatg atgaaataga taatataggt tatccagtat ttataaaacc aaactcagga
    21001 ggttcaagtg tagccacatt ctttattcat tctaaggatg aagttgaaga agcagttaga
    21061 aaaggtctgg aagttgatga atttgtaatg attgaaaaat atataccagg tggagaatat
    21121 acttcattca tattaaatgg agaagtgttc cctacaatat ctataaaatc tgattcagga
    21181 ttctttgatt atgaagctaa gtactcagtt gaaaaaggtg caaaggaaga agttgtttac
    21241 ttagatgaag aacttcaaaa aagagtaaac gaaatttctg agacttgttg gaaaattttt
    21301 aattgcaaag catatgtaag agttgatatg attataagtg aaggtattcc atatgttctt
    21361 gaactaaata ctcttcctgg aatgactcaa actagtttaa ttcctagaag tgctgctgct
    21421 agaggtatta agtacagtga actattagat aaacttatag aatactcttt aaattagatg
    21481 aaataatttg aggtggtaat ttgatatttt caaatctaaa actaaatgat aatgaaccaa
    21541 tttatataca acttaaaaat tatataagtg atatgatatc aaaaggacta attccagaca
    21601 atagtaagct cccttctaca agggagctta gtcagctttt acaagttagc cgaaattctg
    21661 tagttttaac ttatgaagaa ttaaaatcag aaggattaat ctactctatt tcaggaaaag
    21721 gtacatttgt taaatcaaag aataagtctt caaatactac atggtcatta aactgggatt
    21781 gtttagagaa tacatactct aaaaaagcca atgaattgga cattataaaa agtgaaattc
    21841 cttggagctc tgacctgatt tcctttaaaa gtatttctcc agatggagat ttattcgaca
    21901 tagaagaatt aaaaaaatcc tttttaaata gaatttcact agaaggacat aaattactca
    21961 actatggata tgcacaaggt tataagcccc ttattgacta tttactagaa tatatgacaa
    22021 ataagggtgc tgatataagt aataaagata tactaataac aaatggattc acagaaggct
    22081 ttgatattat tatgtcatct tttacccaag aaaaagacta cataatatgt gaaaatccta
    22141 ctcataatac tgctataaaa attatgaaat ctcataacct caatatctta ggtgtagata
    22201 taaatgaaga tggtttggac ttcaatatgc tagaagacaa tttagttaag tataaaaata
    22261 aaataaagtt tgcatacatt acaccctctt atcacaatcc tacaggaatt gtaatgactc
    22321 ctgaaaatag gtacaaattc tataatctta tgaaaaaata caatatagca attatagaag
    22381 atggatttaa tgaagaatta ctctacaata gttctcatat atttcctatc tgttccctag
    22441 ataatatgaa taatggggtc gtatatattg gtagtttttc aaaaatttta tttcctggaa
    22501 tgagaattgg atggatattt gcagataaaa aagtaatcaa taagctagaa agtgtaaaaa
    22561 ggtgcagaaa catacatgta tcctttttgg accaaggaat tttatttgat tatttaagta
    22621 atggaggttt tgaaaaatac attaaaaaaa tacgtaaatt ctatggggac aaatttaact
    22681 ttgcatataa gtgtataaaa aaatatataa aaacagaata tatcttgggt gatggtggtt
    22741 tgcatatatt tataaaacta aaaaatatag atactagagt tttattagaa aaatgttatg
    22801 aaaaaaatgt tatatttatg cctggagatt tattttttac agataactct ggttttgata
    22861 ctttaagact cggattttct aggttgtctt ttgaagacat tgaaattgga ataaaaataa
    22921 ttggagaaac tataaatgaa atgttctagg ttaactaaat attgttagcc tagtttattt
    22981 ttatgaggta taatatatat agtttatatg aatctaatac tgggggataa tatgagtaaa
    23041 aagtcaaaac gaagaaaaat taataaatta aaattaacga ttgtaataac tgtattattt
    23101 cttgctttat tatctatata tgagatactg ttctctaatc ccaacaaaaa aggtaatata
    23161 aagagtcaag aaactatgca aaataaaaat aatacaacag atgattctaa aaataatcaa
    23221 agctctacca aaaagcagga agataataat atcaagccta ctttttcata ttcttcaata
    23281 ccagataata taaaaaataa aatgattggt aaatctatgc ctactgatga acctataagc
    23341 tttgatagtc tatcttattt aaaacttaca tattatggat ttgatgaaaa aactcatcaa
    23401 ggagaaatga tagtaaacag tgaactagct ccagaagtag tggatatttt taaggaatta
    23461 tacgaaaaaa aatatccaat tgaaaaaatt aagttaatag atgactatga tgctgtagat
    23521 gaaaaatcta tgtctgataa taatacctca tcattttgtt atagaactat agctggtaca
    23581 aatgtagttt caaatcatgg taaaggacgt gctatagata taaacccatt acagaatcct
    23641 caagtttctg gaaatgatgt tacacctaaa gtaagtacag tttatgctga taggtctagc
    23701 acaaaatttg gaatgataaa aaaaggtgat gattgttata atgcttttgt aagcagaggt
    23761 tggagttggg gtggctattg gaaaaaccca gattatcaac attttgaaaa ataatagatt
    23821 agaaataaag gtgaattata tgaatttaat gacattggaa aatataagta aaagctattc
    23881 tgaaaaaaaa ttactagaaa atatatctct tggaataaat gaaggggaaa aaataggact
    23941 tattggtgta aatggtactg gtaaatctac cttactcaaa attattgcag gagttgaaga
    24001 aagtgagact ggtaatataa caaaaactaa tggtattagg atagaatatc tacctcaaaa
    24061 tccaacttac aatgaaaatt caactgtact tgaacaagta tttaatggaa cttctgaaga
    24121 attaaagcta cttggtgaat atcaagaaat cctagaaaaa cttaattcaa actttacaga
    24181 ggaattaaat aaaaaattat tatcagtaca cgaaaaaata gatgccttaa atctttggga
    24241 tttagaaagt gaagctaagt cagtacttac aaagcttggt ataaatgact ttaatcaaaa
    24301 aatagcagaa ctttctggtg ggcaaagaaa gagagtctct ctggcatcag cccttattac
    24361 accttgtgag tttttgatac tagatgagcc tacaaaccat ctagacaatg atactataga
    24421 ttggcttgag gaatatctta attccagaaa aggctcattg cttatgataa ctcatgatag
    24481 atacttttta gaccgtgtta caaatagaat tattgaactg gacaaaggta gattattcag
    24541 ttatgatggg aattattcaa tatttttgga aaagaaaatg gaaagacttt cgctagaagc
    24601 aagtattgaa gataaaagac aaaatttaat aagaacagag cttgcgtggg taagacgagg
    24661 agctcaggct cgtagcacta aacaaaaagc tcgtttacaa agatttgatg aacttataaa
    24721 tagagattct tttaaacctg atgaaaatct tgatatatct gttgcatcaa gtagacttgg
    24781 aaataaaata atagaaatac acaatatatc taaatcattt gaagaaaata ctgttataga
    24841 taatttagaa tacactttaa ctcgtacaga tagaattggt ataataggca aaaatggtat
    24901 gggtaaatct acccttataa atatacttaa tggagaaata gagcctgata gtggatatat
    24961 ttcaattgga gaaacggtta aaataggttg tttttctcaa gacgactctc atatgaatat
    25021 tgatatgagg gctatagact atgtaaaaga agctagtgac tatatagaaa cttcagatgg
    25081 tactaggatt acagcttctc aaatgtgtga aagattcctt ttcaatggta ctatgcaata
    25141 tactttgata ggtaaattat ctggtggtga aagaagaaga ctacatcttc ttaaaatcct
    25201 tatgacagct cctaatgtgc ttcttttaga tgaacctaca aatgatttag atattgaaac
    25261 gcttaaaata ttagaagaat acctagacga atttaaaggt gtagtagtta cagtatcaca
    25321 tgatagatat ttcttagata gaatttgtaa taaaatattt gcatatgaag gtaatggaaa
    25381 aattcatatc tatacaggta attacagtga ttatcttatt tatagagaga ttcaaggaat
    25441 tgaatttgaa gaatctaaaa aggaatcagt taaaaaagat gactctacta ccctaaaaaa
    25501 agaaaaacct aaaaatgata aaaagcttaa gttctcatat aatgaacaaa gggagtttga
    25561 aaatattgac tctgatatag aaaaactcga atctaaaatt gcaacacttg atgaaagtac
    25621 tgcaaaattt tcaactgatt ttactaaact tcaagaaatt ttagatgaga aatctaaact
    25681 agaaaaagaa cttgaataca aatatgaaag atgggaatat ttaaataatc tagctgatga
    25741 aatagctaat aataagtaat attaaaataa tcttaagttt aataagtttt gctttaaatc
    25801 tgtaatttat aaatatttta aaggaagtgt ctcaaaatga actttttagt tttatgagac
    25861 actctttttt atgagaaatc aaatatattt tcatcatttc aacaatacaa aagaggctta
    25921 tctctatttt gagacagcct ctttcataat taccttctaa tttatatgct ctaatatatc
    25981 tataataatt cttattatat ctatatatcc agtctttcaa tataatgaca attaacttca
    26041 atatgattta aaaatactaa caacctttca taaagtggat gagattcttc tccatattta
    26101 gcttctaggt tcttgcctat ttctctaaca gtcctttgac catcaatttg taaaaaaaca
    26161 aaactggaat attcatctag ttctattttt ttgtatttag gtattctagc ttttaacttt
    26221 ctaaaaaact tttgaatttt atggtcctgt ttttcaagta tagtaacaac tctattttca
    26281 gctacttcat attcaagtcc atcagtaatt ttaaatacaa tatctaaaac atcattactc
    26341 ttgctcatta ttttttactt tagataaaac aataggtaat gctgtaagca caactaaaac
    26401 tactaataaa gcaattgcca ttgaatttgt tgcagcaaat ccacttggta cctttggagt
    26461 tacaactcca gttacttgta atattatacc tattaaacca atgatagaac caccagcaac
    26521 aagaccagaa gataaactta taccattaga aactttaact tctttttctt tttcagtttt
    26581 agatactaat tcaacaaata aacgaattaa tgctccaact aatattatag aagttgttgc
    26641 aattggtaaa tagaatccta tagctatagt cattattggt aaatttaatg catatagaac
    26701 tattgccata aatactccaa caataatcat aacccaaggt aaactacctg acataatacc
    26761 agaagttaat gttgacatta agtttgcttg tggtaatgca aattgtacat tatcaccagt
    26821 tgctctaagt tggtcagaaa gtaataaaat tactcctaca acaactataa caccaactat
    26881 acttgcaatt gtaaagtaac gttgcatttc gtttttgcta ccaccaataa tataagttac
    26941 tttttgagat tgagtataac ctccagctat agcaatagca acaactataa atgaaccaaa
    27001 taaaagtaat gatttattag cttctaaatc agtccatccc attataacaa acactaatgt
    27061 tacaataact agagaagcta tagtcatacc agaaactgga agatttgatg ttccgattgt
    27121 tcctgttaaa cgaccagcta ctatgacaaa taataaagat aataaaagag atatgatagc
    27181 tccaataatt gccataacaa tgttaccaga aattaagaat gctgctaaaa atccaataac
    27241 tactccacct aataaaagta tcatttgtat agatgaacct tcttctcctt ctccagcact
    27301 tgatttagct ttaagtgttt cttttataga agcgattata gttggtataa gttttattgc
    27361 accaataata cctccacaaa gcatcatacc agcacctata tattttacat aactactaga
    27421 tattgctcca acatccattt ggttaagagg cattgcagga ttattccata ccatagctcc
    27481 atctttagcc atatctgtaa agtatcctat taaaggtgct ataccaaagt ttgataaaat
    27541 acttcctgca aacattgtta aagaaacttc aagccctact ataaatccaa ttcctaaaag
    27601 tagaggattt acttcaatat caaatttcca tttgtagaat ttgcttccta caagactcat
    27661 tacattgttt gctacattta agaatgaacc agttaataca gtaataaatc cacttattac
    27721 aaatccaatt cccatgtact taattgaatc tccaccagcc tctgaagcaa caagtgtttc
    27781 agatatagcc attgattctg ggtacataag tttaccatgt tcttcaacta ttagatagtt
    27841 gtgaactata gcagcaattc ctataccaaa taaaactcca ccaactccaa cagctaagcc
    27901 ttcaaagaat gtaatttggc ttccaattaa tataactgct ggtaatacga aaataaatcc
    27961 acttgcaaca gattctccac cacttgacat accttggatt aagtttttac caagaatacc
    28021 ctttgaacga gcaaatgctc caacaaaagc agaacctatt atagctcctg gaatacctgc
    28081 ggcaactgta agaccggctt tcattccaga gtaagttgta gatgcggcaa aaatggcagc
    28141 taaaataata ccgataatca gtacagctac atttccaccg gttctggact tatctgtgat
    28201 atatggaacg taatcttttc cactaacctc accataagca cccttaggta attttttgtt
    28261 cataaaattt tcaccccttt tcaatttgta tatattataa catatataca tataaaactt
    28321 aactttttat tattttacaa atagcgagtc aaaactacta aaatttaacc ctatattatt
    28381 aatttctact tttagatatt tttatgctaa ttttcttttt atttttcaat tctactaaaa
    28441 actattaaga tataaaataa aaaaattttt taatgtaaaa ttaattttgt aaaaaaaatg
    28501 ctttaaaaca cggaaaagta taaaaaatac aataatcatt cttataaata aattttcacc
    28561 atagtaaata tatacagaaa attcttgttt ttatgcaaat tgattctttt ataaaaataa
    28621 gtgtcataaa gaaatgtctg aaatcacttt atgacaccta tcataataaa aaattattct
    28681 ggtaatgctc ttttatttac ttttccgatt ggtgtaactg gtatcttatc tatcctcatt
    28741 attattgttg gaatcatata tggaggtaga tgctcattta aatattctct aatagttctt
    28801 tcatttatct taccactatc ttcctcatca attacataat atgcacatat atacatatct
    28861 ccatcatcat ttgttctcga tatacaaaca acacttttaa tttctgtaat attatttatt
    28921 atattttcaa tctcatatag ctctatccta aactcatcaa tttttacctg attgtccatc
    28981 cttccaatat aatctaaatt tccatcatct aaccatctta ctaaatcacc agttctatac
    29041 attaacttat tatgttcaca agttttgtaa ggattctcta caaatacttg ctctgttagc
    29101 ccttttttat ttaaatatcc tcttgatatt tgaatgcctg atagacatag ctctcctggt
    29161 gctccaattg gacatagttt gttcttttta tctaatacat atgcataagt attaaatata
    29221 ggttttccta ttggtatttt tccatattct ctgtccacat gaaaagaagt cgatacaaca
    29281 gtaaattcgg ttggtccata ctcattatat agttcataat tacgctttct atatcctttt
    29341 aatctttctc ctccggtctg tacaactcta agtgaattat tattgcaatt ttgtataaat
    29401 aaccttgcaa atgctgttgg caatgctagt atagttatgt cattttcctc acagtattca
    29461 tttactgtat attcatcaaa tttagcttct tcaggcataa tatctattga tgcacctaca
    29521 agcaaataag ggaatagttg tttcattaca gcatcaaaac tgaaacttaa gtatatacca
    29581 catatatcat tttctgtagc gcttatagat tttacgctat aataacacat atttactaaa
    29641 ctcttatgtt ctatagctat ggtctttggc ctaccagttg accctgaagt agatatcgta
    29701 tatgcacaat ttgatgatgt tattttttca gttaattcat atccaagctt aaacatttct
    29761 tcatcattta tatcgagtac tatggtattc tcatttacaa ccttactttt gagttcatct
    29821 gtagttatca ctacttttgc attactatct tcaagaaggt aattcaatct ttcttctgga
    29881 ttacttgtgt ctattggaaa aaatgctccc cctgatttca taatccctat tgctgtaata
    29941 attatgttaa tatctcttgg taacattaca gctactatgt cttcactttc cactcctata
    30001 tgatttaaat aacctgcaag tttatcagtt acttcatcca attctttata tgttaacttt
    30061 ctattttcat ccctagcagc tatattacta ggtgttacct taacttgctc cctaaataaa
    30121 tcaacataac ttttatcata tggtatttca tattgattat tattaaagtc ttccaataat
    30181 actttttctt cttccttaca tacaaattct aaatcttcta ttaaaatatc attatcttct
    30241 attatttgct ctattatata atcaaattga tttaaaattc ttctaatagt ttctctttta
    30301 tatatagatg gtttatactt tatttttatt ttaagtgccc tcttagtata tactattaca
    30361 gataaatcat atcctttttt ttctatgtca taatcatatg ataaatcaaa acttttagct
    30421 ataaatattg tgtttataag cttagtattt agatgatttt tattctctat atctgataac
    30481 aaatagtatc catactctaa agacctgctc cactgccttt ttagcttcct tacaacatct
    30541 ataaattttt cactttgtct aactcttact cttattggaa ttatattcat tgttttacca
    30601 aaaactacat catcttcata tatatacttt tgaagtataa ggccatatat agtttctatg
    30661 atagagtcta tgctaatatt gttcacttta gaatattgaa ttaacctttc atgtttctca
    30721 atattcatct taaaaacctc atctactact tcatcagaac aatctttata aaaaaaatca
    30781 tttggaattt ttgctatatt cgtataatca tctaacaaat ccatccaata attcatagct
    30841 cgttctaaat taacttcata catatcaaaa ttccccctta ctactaagtc tatagaaaaa
    30901 attcttttgg aattttattt tcacaatata taaatatatt aataatacat atgcaagctc
    30961 taaaaacatg agcttgcata aaattacact tattatgact actaaattcg taatatttac
    31021 aatgtttaaa atatattata acacatatat gattttattt ttctacattt atataatata
    31081 taaattacaa atagttattt aactaatata taacaatacc ttttggaata tgatatatta
    31141 tattaattaa atttttataa gataagttat acttatagga gggaaccata taaatgaata
    31201 tttatgctca tagggggttt agtggaaagt atccagaaaa tactattcta gcttttaaaa
    31261 aatgtttgga tatggatata tatggaatag aacttgatgt ccacagaact aaggatggaa
    31321 aaattgtagt tattcatgat gaaaaggttg atagaacttt taatggacat ggttttgtaa
    31381 aagactttac tttaagaaaa ttaaaaactc taaactcatc ttttgaagga tatcaaagca
    31441 ataaagaatg taaaattcca actttagaag aagtattaat tctaatttca cctacagacc
    31501 ttattttaaa tatagaacta aaaactgaca aaataaacta tcccaatata gaaaaagacg
    31561 ttttagagct tattttaaag tataatatga aaaatagagt tcttatatct agtttcaaca
    31621 gcaatagttt aaaaaacttt cataaattag accctagtat aaaaacagga ttattatgtt
    31681 atttacctat aaacaatgta gtcaattttg ctaaatttct gggtaatagt tatcttcatc
    31741 cacctctagt tttagtaaat gaatctctaa ttgaactttg tcacaaaaat ctacttggtg
    31801 tcaatgtata tactgtaaat gaagagggtg atatactaca ctgtctaaaa ctcaatgtag
    31861 atggtatttt tactaactat ccagatattg cttcaaatct tttacactca aaacaataca
    31921 gctaattata caatagtttg tactccttga cttttatata attaatatgt acaattttat
    31981 taaatttatc ttggaggtat aactatggat aacttattaa atgaacttga agctttaaag
    32041 agtaatttag ataatctacc aatagaaaaa atagaaaatt atgatttgag taaaactgct
    32101 ttatttataa ttgatgttaa taatggcttt gcaagacaag gtgcattata ctctcctaga
    32161 gtagagtctt taataaagcc aattgaaatg tttacaaaaa aaatatcaaa caaattaaat
    32221 agagtaatag catttactga ttctcatact cctaaatcaa tagaactttt gagttatcct
    32281 gttcactgtt tggaaaatga tgtagaaagt gaattggttg atgaacttaa gtcgatagaa
    32341 aacctacaaa ttcttcctaa aaattctact aatggatttt ttgcattaga aaatttggat
    32401 tttgataata tagataatat aattatagtt ggcgattgca cagatatatg tatatatcaa
    32461 tttgcaataa ctctaaaatc atattttaat caacataata ttgaaaaaaa tatagttgta
    32521 cctatgaatt tagtagatac ttatgatatt cctaacgttc accctgctga gattttaaac
    32581 ttagtatttt tcaacagtat gattcaaaat ggagttaatg tattaaaaga gattcgttaa
    32641 gcttctaata ctttacatcc catataatac aattatcaga tatttatttt taataaaatt
    32701 tctaatagaa aaacacagtt ttagtgagaa gattacaatt taaaacacta tctattatta
    32761 ctaatcttca caatataaaa aatcgcagtt tagatattcc ttatacaaat ttataaggaa
    32821 taataaattg cgatttttat ttttaagtag tacaattaat ttatactact tcagtattta
    32881 aattcatttt ttaataatca tacttattcg tagtttcgca cattactacc tttatttttt
    32941 tattaaacat attcttatat tctaataaaa actcatttag ttctgaatcc ataagattta
    33001 tttttctatc actacaaagc attatctttt cttggtcgat tattacatta atatcattta
    33061 agtcattttt tataaatgtt gttatacaac tctttggttg gattttttct ccatatttct
    33121 cataaaatcc tgcactcttc ttaaatgact ctaaatcttc tgtagatact ttgctggaag
    33181 tattattact cataaaacct gctgtagaag ggtcgattcc aaagtttcta agttgttttt
    33241 ctacttcttc agtgtccata ccatatctct ccataaattg cttttgtatt ttcataaatt
    33301 tttcttgaga tatgttgtta tctctcatat aatcgaatat ttttcttgaa aactcagcca
    33361 tagacatctt tccatccata acagaaaaat ataagttata tatgtattgc tcaaatttat
    33421 ctacatcaga atctttaact ttgttcttta gttcgttaaa gtctattatt ctatcatcag
    33481 acatttttca tcctcctagc taaattttta cttgtataag attataccat atttctatta
    33541 tatttatata cacactattt ttttaattta acgatttttt ctgtagcatc taattcgcac
    33601 attctgccca aaggaatagt tatcattggg atacaatgtc ctgactgaag attatatata
    33661 gaaggtttat tgaccttatt tgcaaattct attagtaatt ccatcaaatc attttcttca
    33721 atacaatcct taaaatctcc aaaaattata ccattacatt cctcaagttt tcctgaagaa
    33781 tatagttgtg ttaacattct atccagttta tattgaggtt ctccaatttc ctcaataaac
    33841 aaaattttat cttttgcatc tatttcatat tttgttccca ttgtggcaat tattaaggat
    33901 aaatttcctc ccattattat tccctctgct ttaccttcac atacagtata aatcttttct
    33961 tcttttggat tttctaaata taattcatct ttaaattcta aagcatttac caatgattta
    34021 taggtaaatt taccccattt acctacattt gctgccatta ttccatgata tggtaaaaga
    34081 ccacaatttt tatagaaagc taaatttaaa cctgttatat cagaaaaacc tataaatggt
    34141 tttggatttt gctttattat atcataattt attttatcca atattcttgt tgttccataa
    34201 cctcctctta agcacataat tatatctaca tctttatcta aaaacatatc ttctatatcc
    34261 cttgctctaa tatcatcatt accagctaaa taacctttat aatttaaact acaactttca
    34321 gaaaacttaa cttcgtaacc ataactttct aaattaaact ttatttcctc caaacttgta
    34381 tttcttaatg gcccagatgg cgcaattatt cctaacattt aaacacctct tcttataaaa
    34441 tgatatattc ttctccatac taaatatctc taatataatt ataagatatt ttatttgtac
    34501 ttgatacata ataatgattt acataaaata atataattaa tgaagaaaaa taaattttta
    34561 atatagttag gatgtgtttt atgcttaata taaaaactct ttgtcatgag ataaatgatt
    34621 gggttataaa tataagaaga gatttacata agactccaga acttggatta gaagaatttc
    34681 aaactaagaa aaaaattatt aaatacttaa atgaaatagg tataaattat attgaatata
    34741 agaatcatac agggataact gcatacatta acgtgagtcc aaactttgaa acagttgcta
    34801 taagagctga tatagatgcc ttacctataa cagaagaact taattattct tataaatcta
    34861 ttaatattgg caaaatgcat gcatgtggtc atgatgcaca cactgctatt ttactgggta
    34921 cttgtaatat actatttaaa ttaaaggatt acttaaatgt aaatgtaaaa ttcttttttc
    34981 aacctgctga ggaaactatt ggtggggctc aacttatgat agaagatgga tgtcttgaaa
    35041 aaccaaatgt aaaatatata tttggactcc atgtaaatcc caatatcaac aaaaatctaa
    35101 ttgaattaaa atacaacact ctaaatgctt ccaccaatac acttcaatta actgtacatg
    35161 gttcaaaatg tcatggagct tatccacatc aaggtattga tgctattgtt atatctgctc
    35221 atattattac agctctacag acaatcgtta gtcgaaacac aaatccaact gattctgtgg
    35281 ttatttctct aggaaagatt gaaggtggaa tcaaagaaaa tatagtttgt gacaaagtgg
    35341 ttataagggg tactttgaga accttgactc ctgaaactag agagttttca aaaaaacgaa
    35401 ttagagaaat ttgcgatttt acttgtaaaa catttggagg aagtatttct gttgaaattg
    35461 aggaaggtta tccagctctt atcaattcaa accatctagt agattacgtc aaacaaaatg
    35521 ctgtagaatt gtttggtgaa gaaaatatta tccttaaaga ctcgcccact cttggtgctg
    35581 aagacttttc gtattttcta agacactgtg aaggagcttt ttaccacctt ggttgtgcaa
    35641 atcgtgaaaa aaacattact tcccctttgc atacctctac ttttgatatt gacgaagatt
    35701 gccttattac tggtgtcatg ctccatgtaa aaaatgtatt atcattctaa ctattaatat
    35761 ttttatttat tattttatat tgaccctcac atgatgtcat gttataccct aaaattacaa
    35821 atgataaatt aaatagttgg aggtaatatt atgaaagaaa tattaataaa tcctataggt
    35881 aaaatatgta ttcaaggcga agaggtcttt attaaattag acaaaaagta tgtacctgca
    35941 ttaaatgaac tcgacgattt cagtcattta aatgtatttt ggtgggctga tgggtttgat
    36001 aatcctgaac taagaagtat tttagaaacc ccaaaacctt ataaaagtgg accagacgtt
    36061 ataggaatat ttgcaacaag gtcacctata agacccaatc caatagcatt gactgctgta
    36121 caaattatca atatcgacca tgaaaatggc ataataaaaa taccatatat tgatgctaat
    36181 gataatagtc ctgttattga cttaaagcca tatactccaa gcgttgatag ggttgaaaat
    36241 ccgtccgttc ctaaatggtg tagtcactgg cctaagagtg tagaaaaatc aggagaattt
    36301 aattgggaag atgaaattga attttaaatt acttttttct tgatttcatt aacttgtttt
    36361 ccacatttat tttatttaca cttaccaaga ttttatgtca catatacata taatattagg
    36421 ctgtattata taatacaacc taatattata taataaattt ctatttttcc cttaaatcat
    36481 tcacattctt tctaagctac ataagatttt tctattattt ctttttgagt ttaatttagc
    36541 attatgttga ggaaaaaaca tttcatgcat tttaataaat taaatttttc ttttattctg
    36601 cgattttata ataatcaatt taacttaaaa ataataccta actattttaa ctatgttaaa
    36661 ctagtaaatg taaccactta tatgttacaa ttttatataa acatcaaata taaaaaacta
    36721 tataaataaa tgacacatag tgttatattt ttttatacca atttagttat ttttcagtta
    36781 cagatttaat aatagttaat agtaagacat aatattttta tgccctttat tgattatgta
    36841 ttttctgtta tctgttatta tcatcaataa taaacataag ggggtatata ttatgaaatt
    36901 gtatttaagt gctgatatag aaggtacttg tggtattgta aattgggatg aaacaaaact
    36961 tgaatgtgaa ttttcgcaac actatagaac gcaaatgagt aaagagataa cagcagcatg
    37021 tgatggagct actcaatctg gtgtacatga aatacttgtt aaagatgcac atgacagtgg
    37081 tagaaatatt aatccaaata ttttacctga aaacgtcaaa atacttagag gatggaccaa
    37141 agacccacta gttatgatgg caggaattga tgaaagcttt gatgcatgta tattcaccgg
    37201 ctatcactct ggagctactt gtaatggtaa tccactttct catactatgg atattgatta
    37261 tgactatttt aaaataaatg atgaaatagc atctgaattt actataaatg cttatattgc
    37321 tgcatattat aatgttcctg tagcattttt aagtggagat gaaatgcttt gtgagagtgc
    37381 aaaaaaacta aatccaaata tagtcactgt tcctgtgtca aaaggaattg gaaatggttc
    37441 aatttcaatc catccaaacc ttgcactaaa aaaaattgaa aaaggtgtca aaaaagcttt
    37501 atctggagat ttatctaggc acttaataaa attgccagat aaatttaaga tagaaataaa
    37561 atttagagaa cattataaag cttttaaggc ctctttttat ccaaatatga aaaaaataga
    37621 ttctcaaaca gtactatttg aaacagagga ctattatgaa tttttaagaa tgttattatt
    37681 tatataaatt tcatatcata aattaaggag ttgattaaga taagtaataa aagaaggatt
    37741 cctttaggcg aatttatttc tgtattttta ttttttgctg ttttttttgg tataactttt
    37801 tgtattttca cggttatagg aattggcttc ttatcattct taggatttga atataaatct
    37861 cttggagctg tattaatttt ctttttaata tatttttgta taaccactcc aattgatttt
    37921 ctatgcacta ctattttaga tatattcaga tatgtaaaca aattaccata ctcaatatat
    37981 aaactttgcg aatttataat tgattttata ctaacttttc tagctatgaa cataattgat
    38041 acctttatgg attcagtaac gattccttta agtacagaaa tcttattcgc tttactttct
    38101 tatcttttat cagaatgtat ggattttttt gatagggata agaaaaaacc ttaataaaaa
    38161 agatagattc cttaatagaa cacatatttt cgtatttcta ttatttaatc tatctttatt
    38221 ttatttaata aatttttatt aattaaaata tagtttcttc tatctcctta gtcatttgct
    38281 tagcactatc aaaaaattct tctatataca ctacttcatc actgacccta gaatattctg
    38341 ctgcaaatgc cattacatga ctttgtatag cttcttttgc tgctactcta cccttttcta
    38401 gttctttatt tgaaactaat ttaataaatt cagctataag atttctatca tattcctcta
    38461 ccatattctg ttgatttgat ggattcataa ctatttcttc gttttttcca aattctttaa
    38521 ttcttatctc atttttattt aaatccccac caacttcacc atggctaaac attaacttta
    38581 tagttcttcc attttcttta gtaaatgcac ataaattaaa agtagccgtc actccatttt
    38641 caaactgtag tatatttacc atattgtcta caacattatt atcacatctg taaacacatc
    38701 ttccatatgg acttgtctta agtatattca atagattctc ttcagttgga tttatgtgaa
    38761 cagctctatt tatacttcta tctttttcta aataaattct ctttgctgaa tatggacatt
    38821 ttttttcaac taaacactga agacaatttt gtgccatctc tccactagca ttttggttat
    38881 taagatgctt taagcttcca aatgcagata tttttttgca tctacttcct actaaatata
    38941 acaaaatatc catgtcatga caactctttg ctaaaattaa agggctggtt tcattgctat
    39001 ttctccaatt ccctctagta aagctatgtg caaaatgcca atatcctata ttttcattat
    39061 attgtatact aactagctct cctaattttt tgctttctac aatttctttt aatttattat
    39121 aaaatggaga atatctcagt acatggcaaa tcataaaaat cttatcttta tacttatcac
    39181 acaagtcatc tatatgtacc agaccatcta aactattaga cattggcttt tctaatagta
    39241 catgataacc tttttcaaga gctagttttg ctacatcata atgtctatca tcatttgtag
    39301 ctattattac tgcatctgcc atcttgtcat gtttaaaaaa gtcttctaaa gtttcaaata
    39361 catttttact atctaggtta tatttttgtg taaatagttc tcgtcttcct tttttaggtt
    39421 ctgctacagc aactatttca catacatcag aattttcata aatatatgga gcatatgcat
    39481 acgttcctct attacctgct ccaatcacag caactttcac tttttccata ctttaattta
    39541 tcccctttat attatttata ctactctatt atagtctatt tttacagaca tttggatatt
    39601 tttaataaaa gaaacagcag ttataatttt tcaattttaa ctgctgttat aagttaatat
    39661 accaaacatt tttaattaaa ttttctttaa ttttaataca aagacttaaa tattaaatac
    39721 aaattataaa aacactttta tctagtttac ttcttctttt ctttgtctaa tttatctaac
    39781 ttcttattgt ataaaaacat ataaaatgct gccgaaaata ctattaaata tccaacaaca
    39841 cttaacatat ctggatagac attgaataag aatatactta aaattgctga aaagattata
    39901 ttactatagt caaatataga gatttcctta gcaggtgcat atttatatgc tagagttatt
    39961 ccaaattgac ctagacttgc aaatactcca cctagtaata gatatataag ttgcataagg
    40021 gtcattggtt catatactgc aaaagcaatt ggccctacag taactaatga aaaaactgaa
    40081 aagaaaaata caattgtata gtaatcttct ctatctccaa gaactcttaa acaagtatat
    40141 gccattgctg caaatacagc tcctgcaatt cctccaagat atggaattac ttctacacta
    40201 aatgttggtt ttattatgaa taaagctcct ataaatgcaa ttataatagc tcctatctgc
    40261 tttacattta ttttttcttt tagagcaatt gcacaaaata ttactacaag gaagggactc
    40321 attttattta gcatattagc atctgaaagc atcaacttgt caattgcata gtagttaaat
    40381 ataactccta gagttccaaa tattgaccta tataaaagta cttttaaatt ttctttttta
    40441 ccagttagac ttcctttatg ttttatgata agccaaaaag ctattatagc tgataccaga
    40501 tttctaaaga acactttttg aaaacttggt aaatctccag ctagctttac gaatgcagac
    40561 atcattgcaa agccaagcgc tgatgctatt ataaacatta taccttttat tcgatttgat
    40621 ttgattgtgc tactcatacg tacctctttt ctatataaat gtataaagcc atattaatta
    40681 aaaacttagc ttatacatta aataataatt atctaaatat tccaatttat actataatta
    40741 aaagtgtaaa ctatgtcaag taactaataa atcttttcaa ttaataacaa taataattat
    40801 agctaaaaag ttatatgttt ttatggatta ttatcagaat atataatata attttatata
    40861 gtatagactt tttttaattt attatttaga tttttaatag attttttaac tatggaggta
    40921 tttaaaatat ggaaataaag agatatgaag gaactggaag aatgagtaga gctgtagttc
    40981 ataacaatac agtttattta tgtggacaaa ctcatgctga aggaggagta attgaacaaa
    41041 caactgaagt tttagctaaa atagaagatt tattaaacaa atatggttca gataagcaac
    41101 atttgctatc tgttactatt tatttaagag atatgaaaga ctttgaagct atgaactctg
    41161 tttgggatgc ttgggttgaa aaaggatttg aacctgctag agcttgtgtt gaagctagac
    41221 ttgctagaga gcatttgtta gttgaaatga gcgttgttgc agctacaaaa taatcaattt
    41281 agcttatagg aggctatctc aaaacagaga gataagcctc tttttcattg ttgaaatgat
    41341 gaaaatatat ttgatttcgt ataaaagaaa gtgtctcacg aactaaaaaa ttcattttga
    41401 gacacctcct cactatttaa tctatataaa tttacttatt ctctattggc aatatcaatt
    41461 tagatggata tttacctcca tggtaaatcg tattctttgc ttctatatat tcaatactat
    41521 tatatccctc tgtcgtgttg ctattttgga aaataaagtt tttagcacta gacgttatat
    41581 ctagtctaat tttatgacca actttaaacg tgtttgatat ttttgaagtt ataatcgtaa
    41641 acttataaat ctctccttct tccataaact ctgacttata aaaactattt ctaaatctag
    41701 cactaagtat accatcagct aatttaattg aatttccatc taaatccacg tccataattt
    41761 ttataaccca gtcagtatct ttggcagaac ttgatgcaaa aaactctaac tttatatcac
    41821 ctgttacagt aaattcttca ctaaaagcat ctgatgtata acacaacata tcacttcttt
    41881 tttctaaatc tttgtagtta tttggaactc caacttcatt ctctgacata tcaattagtt
    41941 gtactgatgg gtcttttgga ttatatatat aactatcata cttttcttct aaatcattat
    42001 caaatactaa tctaccattt cctaaagatg tgttagcatt tccatcactt attagataca
    42061 tacttttatc tatcttattt attattggcc aattttcttc tgtcttccac ttattagaac
    42121 ctacagagta atactctact ggagcagtcg tatctatgtt attttctata ccttttaagt
    42181 atttatcaaa ccataataga taattataat ccaaatcata tcttaatgaa ctatttccta
    42241 aagaaatacc atttatatct cttaaagtat ttgaattatg catccatgga cctagaatag
    42301 cctttctctt tcctttttca tagtccttta taacatctaa ggcctctgta gttcccatac
    42361 tattatcatc ataccaccca gatacaacca atgcaggtac atttatttta tctttttgta
    42421 aatgccagtc catcaaatcc caatactcat ctttttctac tctctttaac cattcttcca
    42481 gaaattcaat tctatatcca agagcttcaa caggtatatt ttctattggt cgtatgttta
    42541 aaacatcatc ccaatcatct cttaccattt tgcttctatc aactttattt cttgatacca
    42601 taaatgccca tgcaagcata ccagatacaa aagctcctcc ttttcttggt atatcaataa
    42661 aagggcttcc tgatgttact atactaacta aagctttaag atgcttattg cctgaagatg
    42721 cagctgccca ttggacataa ccaagatatg atgctccaag cattccaact attccacttg
    42781 accattcctg tgatactatc caatttatgg tactatctcc atcttccctc tcatgaatca
    42841 taggttccca cttaccttct gattcatttc ttcccctaac atcttgtatt acaacagcat
    42901 atcctctttg tacatattta taatatattt ctttatcatt ttcttttcca tatggagttc
    42961 tcataagaat tgtcggtgct tttttggcag aatctacaaa atcagggaga tatacatctg
    43021 tagaaagttt tataccatct ttcatatcaa tcatataatt tccaccaaat cttatagaat
    43081 atatctcttc atttttatga gttttttccc attcttttaa tacagtatac tcttcatatc
    43141 catccataat taatatagta caagtttctc tagataaaca gataaaacct actattttat
    43201 tatctacaat aattatatct agtgggaact ttttgtttct ttgaacccat atatattcat
    43261 taagtttaga atgcgtttct cctaagttat ttataactgt gtctttatct gtgtttacta
    43321 agcttttagt atatacatca aaattattaa ataaagactt taaatctatt tttgtaagat
    43381 ttaaataata tgtattttct tctttgctta ttggttttaa actttctatt ttatttgtaa
    43441 aaggattcaa cttttcaatt aataaatctc catcaaagat tacatttcca ctgtatagtc
    43501 cagatatgta taatttaaat tttatcaatt aaactccccc ctataatcct agagacatct
    43561 ttattgcata tgccactgat atacctgcat aatttcctaa tactccacca attactccca
    43621 ttacgactgc tacacttaca agcgaattcc actttgcaga agcagctact aaagctgaag
    43681 taggaccatc agcaattgct cctactatag aaagtattac atattgatac tttatcttta
    43741 aaagtctagt tatgcctaaa tggaaaacaa atgaagcaaa aattatacta aaacagtaaa
    43801 gtgttatagt aaaagttgaa cctaaaaact cttttatatc tactgaaaaa cctattgtac
    43861 ataaaaagaa taatgcaaca aataatccta aatctaaatt tccctttagt ttctttactg
    43921 gctttaattg agcaattata attgaaattg ttgtaactaa aagtattctt gctgcactac
    43981 taaaacttga attgaaatat ccagcaagta ttgttgaaac ttctgtaaca acaaaaccta
    44041 tagccaacat ccaagctata tctttaatac tccattcttt tgactccata aattcttcat
    44101 tttgacaatc ttctaattct gattcttcta catgatatgg ccaaagtttt ttaaacttct
    44161 ttgaattttt taatattgct ggtagagcaa acataaatat caatgttggt actccaatta
    44221 catagtctgc tgcatttgct gctgcaagag tttgtctact tacattaaga cctgttccaa
    44281 tagctgtaag attagcactt ccaccagtat atgtacctac aaacattcca gcaaccttcc
    44341 aaccttctga tattttttct gcaaatacta atccaaataa aaatgccatg atacttacag
    44401 ttaatacagc tacaaaaatt gcaattaaag gttctcttga taattttgtc atttctttta
    44461 agtcaacact catcaacatt atagtcatag aaattggaat agcatatgtt gaaatagttc
    44521 catataactc agtacttact ggaactactt ttaagtttga aagtattatt cccattatga
    44581 ttattgtaag tgctggtcct aagcttttaa tggctttgta tctttgtaag taaaaaccca
    44641 atgcaatcat tgatgtcatt acagcaagta tactacctgg atttgaaaaa agtgtattca
    44701 tatttaacct ccaaatttat cataagttta cttctattcc taaacctggt ttattgacaa
    44761 gattcataat atctccatcc attacaaatc ctccagatat accttctaat tcttcacaaa
    44821 acataaaact atcaaggtca gcttctgtta tgttcttttt agatgctatt aaatttgctg
    44881 ctgctgttat accaattctc gtttccagca tacatcctaa catacaattt actccacttg
    44941 cctcagctat gttgttgatt tttatagcag gatatattcc acttgatttc atcagcttaa
    45001 tattaaatgt atcaactgca ttctttttta tagccttaat tgcatctatt ggtgaatgta
    45061 atgattcatc tatcataatt tttgtgttac ttttatttct tatgtaagca gtaccatcta
    45121 aatcccaatg tggaagtgct tgttcaattg catcaacatt aaattcttct aactttttga
    45181 tagtatttat actgctattt acgttccatc cttgatttgc atcaattcta attcttatac
    45241 tctctccaac ggcttctcta attaacttta cagcttcaat atcgtcttca ggatttatac
    45301 ctgctttaag cttcaatatc ctaaaacctt cttttactct ttctaaagct tctactgcca
    45361 ttttttctgg cttatctata cttatagtaa tatccgtttg gactttacta tcaaatcctc
    45421 caagaacttt ataaagaggt gcattcataa tttttccttt aatatcatat aaagctatgt
    45481 caatagctgc ttttgcagaa gtattaccta taataattcc atccatgatg gtatgtatgg
    45541 tttcaatatc tagtgggtct ttgcctatta actctttttt aaacataagc aatactgaaa
    45601 gaacattatc caaaacttca cctgtaactg ctgcaagagg tgctgcttcg ccaaatccac
    45661 aatacccttc atcagtttca atttttaaaa ttatactttc tccatactca attactccaa
    45721 atgaaactac tacaggtttt tttaatttaa ttctaagctt ttcaaatttg atatctgtta
    45781 tcttcatatt accctcctta ttttttgaag ttataattca atttttaaaa ttttatactt
    45841 taaattatat tattacttca atatatatgt caagataatt tactgaaaat ttactctttt
    45901 ccaaaatata atgtgataat cttaattcaa ttaatactct tctattaaat ttttttatta
    45961 ttattttctt tgtattaatt taacaataat tcattttcat agacttttat ttggatttaa
    46021 ttaaatttta attgtcaaaa tctactatgt attcttattg ccactacttc tatgttgtgc
    46081 tataattact ttgttttaag cgaatatttt atattaattt tattatttgg aggttcttat
    46141 gctaggtgaa aagatgagaa atataagaaa aagcaagaaa aaaacattaa gtgatgtttc
    46201 caaattgaca gatttatcta taagttatat ctctcaaata gaaagagatg ctattgaacc
    46261 atccttgtct tcattgagaa aaatcgctga agttttagac actcctttat atatgtttat
    46321 ggatgacaat aatactgatg atttagtcat tagaaaagaa gatagagtta tgatgaaatt
    46381 tccaaagagt gaaatgtttt acgagatagt atctccaatg cctacaactg agtttacacc
    46441 ttcaatttta tttgtagagt ttgaacttaa accagaaagt gaggatgcca aagattatat
    46501 atatcatgca tcagaagaaa ttatggtggt aacgagtggt gtggttgata tttgtatggg
    46561 tgaaaaggta gttagattaa atccaggtga ctcaacattt ataaaagcta atgtaccaca
    46621 taaaatcatc aatccaagta aagatacaat agctcgtgga tatggagtta tatcacctcc
    46681 aatttggcca ataaaatcta aataaactca atatgacaca cctacttttt gtagataata
    46741 taatagattt aaatattgct tttattttaa atttaaaata tttactcaaa ctaaaaaaat
    46801 agctatttga ctaatctaaa ccatcttaat tatggtaaat agtaaatagc tatttgtaag
    46861 ttatattata aattataata aaaattgtat ttgttaaaat taaactgcta tatcatttaa
    46921 tacttttgcc tttacaactc ttatattttt catactcttg tagatatctt gaatcttatc
    46981 ttcttgctct ctcaatagtc tatgtgcttc atatatatct acaagttcaa attttactac
    47041 atcatttggc tttgcttgtg ctaacttata caaatctaca cttataacgt tccctatctt
    47101 agtatagcca ccactagtct gtctatctga taacattata atcggttttc catgaccagg
    47161 tacttgtata gctccataag aaataccatc agatataata tctgctccat taaggtgctt
    47221 tatactctct ccatctagtc tacaacccat tctatctatt tcattcgtaa ctttgtattt
    47281 atttgataaa aatttctcta cttcaccgtt agaaaaagca tcaaattgag gaccttttac
    47341 aattcttagt acgatttctg aactataagt tggaatatat tttgaactta attttttatt
    47401 tattgtaaaa tctttttcct gaataaaagt atttatataa tctccttttt taagtgctct
    47461 accttcaaat ccaccaattt tagctcttgt ataagttgac ttacttccca ttgcaagtgg
    47521 aacatctatt ccaccagcta ttgacacata ggctctagca cctgatttca cacctctaaa
    47581 tgataataca tcacctggat ttatttgata tgacctccag ttttgaattt cttttccatt
    47641 tattttagcc cctaaatctc ccccagttac tgctatttgc gtatattcat caaaggtaat
    47701 tgtaggacct aacattaaaa cttctaaaga agcctcatct tcagaatttc ctactaatat
    47761 atttcctact ctatgtgcat actcatccat tgctccacat ccagatacac catattgacc
    47821 atagccaact ctacctctgt cttgtattaa tgtttggaaa cctcctaatt caactttgaa
    47881 tcccatatat accactccct gtactataac gatatttaaa tacttaagtt atttttttcg
    47941 atttctataa attcatctaa tgttattgga acaaatttta taaaatctcc agctctaagc
    48001 aaaatctgtt cttctctatt ttcatcatat aattttaaag gtgtcctacc aattatttgc
    48061 catccaccag gactatcaat aggatataca ccagtctggg aacctgctat tccaacactc
    48121 ccaccataaa ttttagtcct tggcacttct aatctaggtg tagctattct ttcatccata
    48181 ccacctagat atggaaatcc aggagtaaaa cctagcatat atatcaagta ctctctgctt
    48241 gtgtgtattt ttataacttc atctacagtg agattattgt agcttgcaac gtttttaata
    48301 tctataccaa aaacctcatc ataacatact ggaatctcat gtattttagg ttttggtaat
    48361 actatacttt ccaaattatc ttctattttt tttaaattct caatcaaact atcaaaatct
    48421 atttctaatg gattataact tatcataagt gacctatatg ttggtaccat ttctgttaca
    48481 atacctttaa gattcgatat ttctattgct ctcataaatg aaattacttt tttgtttata
    48541 tcttctgata tctcatttcc aaattcagca accactgcct tatcccctga tatcaagtat
    48601 ctagtttcca taaatgtcac ctcttctaaa ttaagcaaat aaagcaccta ggctgctaag
    48661 agaagttatt cctgtatatg cagatattaa aactactatt aatccaaata ttaaaagcca
    48721 tgtaggatgc ttgtaatctc caacaatatc ttttctttta gatgctataa gcataattcc
    48781 taatgtgatt ggaagtatca atccatttaa agctcctgct aaaattaata aagtagctgg
    48841 ttttcctata aatgccatta taagtgttga tattgctata aatgctatta taaaatattt
    48901 ttcatattta tctataaaag gatttagtgt ctttaagaaa gaaactgatg tataagcagc
    48961 tcctataact gatgtaattg cagcagacca taatacaagt ccaaagaatt tatatcctat
    49021 tgctcctgca ctaaatttaa aagctgaagc agctggattt tctgggtcaa gttgaagacc
    49081 ttttgaaacc actgccagta ttgctaaaaa taaaagaact ctcatcattg tggcaactaa
    49141 tatacccaaa agagaagatt tagttatttc ttttatattt tcttctcctg ttattccacc
    49201 gtctattaat ctatgtcctc cagcaaaagt tatataacct ccaacacttc ctccaagtag
    49261 agttattatt gggaatatca gtccttttgg attttctggt tttaccattc ttgatactgc
    49321 ctctccaaca ggaggatgat ttgatattgc tacatatcca actataatta tcattcctaa
    49381 tgctaaaaat ttagttatct tatcgactag agaatttgaa ttttttgaca taaatacaaa
    49441 tatggctatt aatccactta atacagttcc taatgtcata ttcatattaa acattacatt
    49501 cataccaagt gcagctccac caacatttcc tatattaaaa gctaatcctc caagagctac
    49561 taaaaaagct actaaataac ctaatcctgg tattatttta tttgctatat cctgacctct
    49621 aagtcctgat acaccaatta ctctccatac atttacttga gcccctataa aaaggattgt
    49681 tgtaattaaa attacaaatg aaaaattcgg tccaaaatct tgggtaaatt gtgcagtctg
    49741 agttaaaaac cctggtccaa ttgctgatgt tgccattata aatgcagccc ctataagtgc
    49801 tccgacatct tttttatttt tagttttatc tgtaatattt tttgtactca tattttatct
    49861 ccccctcatg ttatcagtct tatactatgt tctctgacga acaaacttct atgttttcta
    49921 atggacaaac ttctatactt tctaattcaa aacgctttct tatttctttg acaaactcta
    49981 ttgcttttgg attgtcccca tgtacacata tagaatctgc tttgatatgt ataacttcac
    50041 ctgttaaagt ttctacagtt ccttctttta ccatcctaac aaccctatct attgcatgtt
    50101 ttgtatcatg tataactgct ccttctacat ttcttggaac caaaaaacca ttattatcat
    50161 aagctctatc tgcaaatact tcatttgcaa acctaagtcc aatttcttta gcagaattta
    50221 tcatttcact attatatagc cccaacagta taatatcttt atcgacttca tatatagctt
    50281 cacaaatccc catagctaac tctttatctt tagctgccat attgtataat gctccatgtg
    50341 gttttacatg ttgtatatta catccatttg aacttgcaaa tgccattaaa gcacccaatt
    50401 gatattttgt ataatcttta gcttcctgct ttgttatttt catctcacgt cttccaaatc
    50461 ctattaaatc tagaaaacct ggatgtgcgc ctattttcac tccatttttt ttagctagct
    50521 gtactgtttt ttccatatga ctagggtcac ctgcatgaaa cccacaagct atattagctg
    50581 atgaaatata ttttaaaact tcttcatcta aaccaatctt atacgttcca aaactttctc
    50641 ctaaatcact atttaaatct actttataca atttatgacc cctccttgct aacattttga
    50701 tagaataagt agttttaatt tttaaatttt gtttatttta tatattgcat gaaatatttt
    50761 atcacttgta ttcaatttta tttttgcaac tattctattg catttaatta atataatttt
    50821 accatttaag gaatattttt gcaattattt taattgaaca ttcatttgta tttctttttc
    50881 aaaaaatctt tttaccattt tttagttaat aattaattat tttcacttaa aataagggct
    50941 ttaaccttta tttttaaagt atttttcatt tttaatcact ttatatgcaa gacagaatca
    51001 tttttcttca ttcatttgtt tctttcatct atataattat cattctttgt aagtaaaata
    51061 tctttatatt tttataatta attctttata tagttttaag tataataaaa tcatgtatac
    51121 taaatgcatg ttaaaatatt attttaaaac atgcacaata tttgtacata ttgtagacat
    51181 ataaacctat aaattataca atagtattgt taagattata tattttttta acataaattt
    51241 tgtattgggg gaaaaacaat ggataaaaac tatacgatgc caatatacca aaaaatagcg
    51301 ttggatattg caaataaaat atacacgggt gaaatacaag aggattcagt actatttgga
    51361 cgctctgttt tggctggtaa atacaatgta tctccagaaa cgataagaag atctgtcaaa
    51421 attttagagg acattggcgt tgttaaaagc ataaagggta aaggtgttat tgtactatct
    51481 ccagacaaag cttcttcttt tataaaaaaa tatagagata ttacaaatat atcatcatat
    51541 aaatcaacac tttataattt aatagatact aaatctaatt tagaaaatga aatattagat
    51601 actataaata aaattttaga ctattctaac agacttgaaa taatcaatcc tttagtgccc
    51661 gttcagttca ccataaattc taattgcaaa tatattggtc aaacagctgc acaaactaaa
    51721 ttttggcaaa atacaggtgc tacaatagtt gcaattaaaa gaggtgaaga acttataata
    51781 tctcctggac catatattga atttttagaa ggagacattc tcttagtagt tggtgaccaa
    51841 cacatttaca attctatacc aatgttttta tatgaaaacg aaaaataaat tatataattt
    51901 agtatgatta tataaaaatg caaaattaac aacaaaaaaa gagcttcata ttttacataa
    51961 agctcttttt aaattcaaat aaatttaact ttgattaatt tttaaatatc caattcatct
    52021 tctttttccc agcttaaagc tctcgataca gcttttttcc atcctttata taatttacat
    52081 ctcttctctt cactcatatt tggattaaac tctttttcta ctgaccactt tgaagtaatt
    52141 tcatttcttc caatatagaa tccaacagac aaccctgcta aataagcagc tcctaaagct
    52201 gtagtttcta ctattttggg tctatctata tctacattta atatatctga ttgaaattgc
    52261 attaaaaaat tattattact tgctccacca tcaactttta atgattttag ttttaatttt
    52321 gaatcgtttt gcattgcttc taaaacatcc tttgtctgat aagcaattga ttccaatgtg
    52381 gctcttacta tatgttcttt ttttgctcct cttgtaagtc ctaaaattga acccctagca
    52441 tacatatccc aatatggagc tccaagacct gtaaatgctg gcactacata tacaccatta
    52501 gtatcctcta ccctatttgc atacatttca ctatcttttg cagattcaat tatctttaat
    52561 tcatctctaa gccattgaac agatgctcct ccaataaata tacttccttc taatgcatat
    52621 gttacttttc catcaactcc ccaagcaatt gttgtaagta acccatgctt agatttaact
    52681 atgttactgc ctgtattcat aagtaagaaa caacccgttc cataagtatt ttttgcagta
    52741 ccttcttcaa cacaattttg accaaataat gctgcttgtt ggtctccagc acatcccgca
    52801 ataggtattt tagctcctga taacatgcgc tcatctgtat gaccataaac atagctagaa
    52861 ggttttacta atggcaacat gcttattggt atgtccaatt ctcgtaatat atcctcatcc
    52921 cattctaaat catttatatt gtacatcatt gttctagatg catttgagta atctgttaca
    52981 tgtacttttc cacgagttaa attccatatt aaccatgtat ctatagttcc aaataataac
    53041 tctccttgtt ctgctttttc cctagctcct tctacattat ctaatatcca ttttactttt
    53101 gttgctgaaa agtatgcatc tattaaaaga cctgtttttt cttttattga ttcttccaat
    53161 cctttatttt taagttcatc acatatttct gacgttctcc tacactgcca tactattgcg
    53221 ttataaatag gtttaccagt atacctactc caaacaaccg tagtctctct ttgattagtt
    53281 atccctattg aagcaatttc ttctggtctt atacctgctg tttctataac ttctctcatt
    53341 accccacttt gtgaccccca tatttccata gggtcatgct ctacccatcc agcttttgga
    53401 taaatttgat tgaactcttt ttgtgcaatt ttcactattt cgcctttttt attaaatagt
    53461 atagctcttg aactggttgt tccttggtct aatgccatta catacttctt ttccatcacc
    53521 atattctcct tatcatataa tatttaattt attaaaaaga aaaatttaat atcacatatt
    53581 tcaataaata atcatactta agtacaaatt taatataata gatatttagt aagaagacac
    53641 ttattttcta tcatatctat ataataatat tatacattat agcttacata ttgattaata
    53701 aattttaaag gagtataata aacagtgttc ataataattt taataaaaga ggtggtaata
    53761 tgccaaagat tttagaaaat gtaaaagaag atatattaaa agtgtctcgg gatatgattt
    53821 tagaagaaga ttactcgaat attagcataa ggaaaatagc tcaaaggtgt ggtataagtg
    53881 caggtacagt gtataattac tttaattcaa aacaagaaat aatagaatat atcacaaaat
    53941 ctgaatggga tttattgata aggcgaattg aatactccaa taaaaacact gaagattaca
    54001 taaaaaaact aaacattatt tttactgaaa taagaaattt tataaacaaa gttcataata
    54061 tccaatacaa tgacttttta aacacttttg aaacagagag attttttgaa atgaaaaaac
    54121 acaaagacga ctttcatgaa cagctttcaa ataaagtata tgaagcttta gaaaaagaaa
    54181 gtttttttaa taatgacaaa ttagtatgca atatcataat aaaaatgttt ttttcatatt
    54241 caacatatct tcatatagaa tttgaagatt taaaacccta tattgaaaaa ttaatacatt
    54301 aaggtggtgt ttaccttgaa tcataatact agtattaaga acaaggacag taatccaaaa
    54361 atatttttag tacttacact ttatctatta ggtatattta tgggagctat tgatactgga
    54421 atagtatcac cagcaagaac gattatacaa aatagccttg gagtaaatga aaaaacggga
    54481 atttggatga ttaccatata cacattaaca tacgcttctg taattcctat ctctggaaaa
    54541 ttagcagata aaataggaag aaaatatgtg tatctagtta gtatatttct atttggtagt
    54601 ggttcattaa tatgtggatt atcatcacta ttttccaatt tctatatttt attaattggt
    54661 cgtgttattc aagcaattgg tggtggtgga atcatgccaa ttgctacagc agaatttgga
    54721 actaccttcc cagaaaacaa aagaggcatg gctttaggtt tagttggggc aacatatggt
    54781 attgccaata ttttaggttc aagcattggt agcacaattc taagtatatt tggaactcaa
    54841 aattggaagt ggctattttt tgtaaatctt ccaatttgtt taatcatact tattggtgga
    54901 gttttctgta ttaaaaacaa taagtctgaa tctaaagaaa aaatagataa gcttggtaca
    54961 ttgatgttga tatgtataat cgtatcacta ttatatggtc ttatgaatat agactttttt
    55021 aattttaaaa attctataca agatatatca gtttatccat atttattgac atttattgta
    55081 cttataccta ttttcatatt tattgaaaat agagctaaag accctatttt aagctttgaa
    55141 tattttttaa atccgagaat attgatagta cttattttat ctttaattgt tggtattgga
    55201 atgatgggta tggtattcgt tccacagtat gctgaaaatg cattaaagat aaatgctggt
    55261 tctggtggat attttgtagc tatattggga ttatttgcag gtattgctgc tcctttaagc
    55321 ggaagactta ttgacaagtt tggggcaaaa aaaatactat tactaggatt ttctgtatct
    55381 atgataggct ctctttacct agttttaatt gctctaaaaa ccaatactgt atttagtgta
    55441 tgtgtaagtt taatatttat gggattagga atgggattta ctatgggaac tcctctaaac
    55501 tatatgatgt tatcaaatac aagattagaa gaatctaatt cagcacttgc aacattatcc
    55561 ttgatacgct caattggtac ttcaatttct ccagctataa tgattggatt tatagcacat
    55621 gcaggattat ctgtgcaaga taatattatg gacttagttg gaaagccctc aactcctaaa
    55681 atagttcaac ttgaagaact tgaaaacatg atagatgact taaaatcaga ccctgaaatg
    55741 gcaaaaaatt taaaaaatgt ctctatccca aatatgaatg atacttcaaa tataaatatg
    55801 gacatgacag gtggcaaact tccaaaatcc ttacttaata aagttcagtc agcagataca
    55861 accaacatta ccaatataac aaaagaaata tcaacaacaa tgtttgatga aaaagttcca
    55921 tctataatta ctaaaataca gaaaaatgta caaaaaggaa tagatggaac acaaagtgga
    55981 ataaacggta ttgaagatgg tgaaaataaa ctaaatagtg gtattaaagg agttcaaact
    56041 ggtattgaca atatggtaaa agctagagct ggtattaatc aaggaataga tggtgtaaaa
    56101 aaaggtattg ctggtgttga caaaggtata aaaggtatgg aacaaggtct tgaaaaacaa
    56161 gatgaggcca tcaaggaact tacaactgct tataataaaa tacctactac agaaaaaact
    56221 cctactgata acgaaaataa ctcaaacgaa caaaaccaga gtaataccag taattctgaa
    56281 tcagatgaca ctaagaatga ttctacagaa aataagaata acaattccag cgagaataaa
    56341 ccaaatgtaa atgaacaaaa agaaagttta aatgctcaaa tacagcaact aaaaaagaca
    56401 agagatgact taaatacaaa gttacaaaaa aataaaactc aaaagaacga attaagtaaa
    56461 aaactaagat caatggaatc tcaaaagaaa gagctacaaa gcaagttaaa taattctata
    56521 agccaaaaga aagacatgga aaaaagcctt gatacaatgc aacagcaaaa aaaatcactc
    56581 caagcagttt tagcaaagac acaagaggta aaaactgaaa taccaaaggc ttttgataaa
    56641 tcaaaattgg attatataaa ttccatagaa aataatagaa caaaaataga aaacacacta
    56701 caatctactc taaactcagg atttaaacag atgtatataa cagtattctg tgtaaatcta
    56761 ctagcattta taatattatt gttctataaa gaaaataaaa ctagataa
//
LOCUS       DAMJWU010000010        54464 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMJWU010000010 DAMJWU010000000
VERSION     DAMJWU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.010"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            489..1328
                     /locus_tag="PC460_000805"
     CDS             489..1328
                     /locus_tag="PC460_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF2599704.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1325..2506
                     /locus_tag="PC460_000806"
     CDS             1325..2506
                     /locus_tag="PC460_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599705.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2810..3712
                     /gene="ptb"
                     /locus_tag="PC460_000807"
     CDS             2810..3712
                     /gene="ptb"
                     /locus_tag="PC460_000807"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF2599706.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3734..4813
                     /gene="buk"
                     /locus_tag="PC460_000808"
     CDS             3734..4813
                     /gene="buk"
                     /locus_tag="PC460_000808"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2599707.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4803..5480
                     /locus_tag="PC460_000809"
     CDS             4803..5480
                     /locus_tag="PC460_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2599708.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            5523..5738
                     /locus_tag="PC460_000810"
     CDS             5523..5738
                     /locus_tag="PC460_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2599709.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5767..6846
                     /locus_tag="PC460_000811"
     CDS             5767..6846
                     /locus_tag="PC460_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2599710.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6846..7598
                     /locus_tag="PC460_000812"
     CDS             6846..7598
                     /locus_tag="PC460_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2599711.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7599..8156
                     /locus_tag="PC460_000813"
     CDS             7599..8156
                     /locus_tag="PC460_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2599712.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8326..9672
                     /locus_tag="PC460_000814"
     CDS             8326..9672
                     /locus_tag="PC460_000814"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF2599713.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9939..11771
                     /gene="glmS"
                     /locus_tag="PC460_000815"
     CDS             9939..11771
                     /gene="glmS"
                     /locus_tag="PC460_000815"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF2599714.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12222..13517
                     /locus_tag="PC460_000816"
     CDS             12222..13517
                     /locus_tag="PC460_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF2599715.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADNENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLIKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFTSNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNRQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            13967..14704
                     /locus_tag="PC460_000817"
     CDS             13967..14704
                     /locus_tag="PC460_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF2599716.1"
                     /translation="MKRVKLAISFFILMIIGMTISYADTSINTKYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGRTLIYIATPIIKLDY"
     gene            14725..15978
                     /gene="murA"
                     /locus_tag="PC460_000818"
     CDS             14725..15978
                     /gene="murA"
                     /locus_tag="PC460_000818"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF2599717.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            16065..17129
                     /gene="spoIID"
                     /locus_tag="PC460_000819"
     CDS             16065..17129
                     /gene="spoIID"
                     /locus_tag="PC460_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF2599718.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKAESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            17195..17863
                     /locus_tag="PC460_000820"
     CDS             17195..17863
                     /locus_tag="PC460_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF2599719.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            17961..18221
                     /gene="spoIIID"
                     /locus_tag="PC460_000821"
     CDS             17961..18221
                     /gene="spoIIID"
                     /locus_tag="PC460_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF2599720.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            18418..19437
                     /locus_tag="PC460_000822"
     CDS             18418..19437
                     /locus_tag="PC460_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2599721.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDNRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            19453..19881
                     /gene="fabZ"
                     /locus_tag="PC460_000823"
     CDS             19453..19881
                     /gene="fabZ"
                     /locus_tag="PC460_000823"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF2599722.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRRNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(19992..20501)
                     /gene="yyaC"
                     /locus_tag="PC460_000824"
     CDS             complement(19992..20501)
                     /gene="yyaC"
                     /locus_tag="PC460_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2599723.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      20690..20793
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20929..22122
                     /locus_tag="PC460_000825"
     CDS             20929..22122
                     /locus_tag="PC460_000825"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF2599724.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEDTKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            22437..23534
                     /locus_tag="PC460_000826"
     CDS             22437..23534
                     /locus_tag="PC460_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599725.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMTIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            23798..26017
                     /locus_tag="PC460_000827"
     CDS             23798..26017
                     /locus_tag="PC460_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF2599726.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIANKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHVEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            26020..26802
                     /locus_tag="PC460_000828"
     CDS             26020..26802
                     /locus_tag="PC460_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF2599727.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKIVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKDNVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            27134..29047
                     /locus_tag="PC460_000829"
     CDS             27134..29047
                     /locus_tag="PC460_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2599728.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIGGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMFTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSSNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIRIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            29188..29496
                     /locus_tag="PC460_000830"
     CDS             29188..29496
                     /locus_tag="PC460_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2599729.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            29520..29846
                     /locus_tag="PC460_000831"
     CDS             29520..29846
                     /locus_tag="PC460_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2599730.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            29885..31219
                     /gene="celB"
                     /locus_tag="PC460_000832"
     CDS             29885..31219
                     /gene="celB"
                     /locus_tag="PC460_000832"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF2599731.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGSDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQVVLLILGIFMYIPFIKMLDKQYLREENATVEEEDDDISFDDLVL"
     gene            31230..31748
                     /locus_tag="PC460_000833"
     CDS             31230..31748
                     /locus_tag="PC460_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF2599732.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGIMMEQFLNE
                     SKVIATLFCGDEFFVNNEEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            31844..32803
                     /locus_tag="PC460_000834"
     CDS             31844..32803
                     /locus_tag="PC460_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF2599733.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            32852..33904
                     /locus_tag="PC460_000835"
     CDS             32852..33904
                     /locus_tag="PC460_000835"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2599734.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            33907..34581
                     /locus_tag="PC460_000836"
     CDS             33907..34581
                     /locus_tag="PC460_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF2599735.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            35134..35685
                     /gene="raiA"
                     /locus_tag="PC460_000837"
     CDS             35134..35685
                     /gene="raiA"
                     /locus_tag="PC460_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2599736.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            35817..38492
                     /gene="secA"
                     /locus_tag="PC460_000838"
     CDS             35817..38492
                     /gene="secA"
                     /locus_tag="PC460_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2599737.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            38507..39614
                     /gene="prfB"
                     /locus_tag="PC460_000839"
     CDS             join(38507..38581,38583..39614)
                     /gene="prfB"
                     /locus_tag="PC460_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF2599738.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNKQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            39758..41899
                     /locus_tag="PC460_000840"
     CDS             39758..41899
                     /locus_tag="PC460_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF2599739.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLEKLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMPPHRVL
                     AINRGEKENFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DNEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKTIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNICEIDEKIKEIGLRELSEKLEVGQVTLKDIITEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            42047..43213
                     /locus_tag="PC460_000841"
     CDS             42047..43213
                     /locus_tag="PC460_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2599740.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      43273..43390
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            43514..44119
                     /locus_tag="PC460_000842"
     CDS             43514..44119
                     /locus_tag="PC460_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2599741.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPFVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVILSLVTLPLYKKMGNVIKK"
     gene            44448..45488
                     /locus_tag="PC460_000843"
     CDS             44448..45488
                     /locus_tag="PC460_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2599742.1"
                     /translation="MNLSKKIKKLFLDDKGYYFNSNWKDIHIGIYNTKLGDSLKYITN
                     EYIENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYICLEAIKKDCNALKYVRNKTERLIIKVIRHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVENQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDIYIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            45627..46079
                     /gene="tsaE"
                     /locus_tag="PC460_000844"
     CDS             45627..46079
                     /gene="tsaE"
                     /locus_tag="PC460_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF2599743.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDDGREMILTPKGEFYKEIVEELIK"
     gene            46079..46795
                     /gene="tsaB"
                     /locus_tag="PC460_000845"
     CDS             46079..46795
                     /gene="tsaB"
                     /locus_tag="PC460_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF2599744.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            46785..47261
                     /gene="rimI"
                     /locus_tag="PC460_000846"
     CDS             46785..47261
                     /gene="rimI"
                     /locus_tag="PC460_000846"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF2599745.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            47267..48283
                     /gene="tsaD"
                     /locus_tag="PC460_000847"
     CDS             47267..48283
                     /gene="tsaD"
                     /locus_tag="PC460_000847"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF2599746.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKKGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKEKGYNIITLSGGVAS
                     NSGLRAKITELAKDSGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            48580..51288
                     /gene="hpdB"
                     /locus_tag="PC460_000848"
     CDS             48580..51288
                     /gene="hpdB"
                     /locus_tag="PC460_000848"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF2599747.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKSAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATADGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            51292..51549
                     /gene="hpdC"
                     /locus_tag="PC460_000849"
     CDS             51292..51549
                     /gene="hpdC"
                     /locus_tag="PC460_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF2599748.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            51542..52492
                     /gene="hpdA"
                     /locus_tag="PC460_000850"
     CDS             51542..52492
                     /gene="hpdA"
                     /locus_tag="PC460_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF2599749.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLGELQDIFLDNGIACYVGHETAF"
     gene            52807..53769
                     /locus_tag="PC460_000851"
                     /pseudo
     CDS             52807..53769
                     /locus_tag="PC460_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(53868..>54464)
                     /locus_tag="PC460_000852"
     CDS             complement(53868..>54464)
                     /locus_tag="PC460_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2599750.1"
                     /translation="IIYSSNKKNLYPIKNIKLGSLLIIYFMISVIVPTSFILILQGSQ
                     PNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFNKFGKKLGVIILGICWSLW
                     HLPLIFTLYTPETPILGLILRSIHIVGISIFLGYLYIKTKNIWFCAIIHILNNSAFLI
                     TNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT        21942 a         5791 c        10319 g        16412 t
ORIGIN      
        1 gttatacata ataagtttta atgatagttt atagtaagat attaaaataa atttatctaa
       61 tctagatatg aaataagtta ttactttaat ataaaaatga atctaatgga ataatattga
      121 tgataagtaa tatttagatt ttgtatttgt ttatagttaa taagtggatt atagaattta
      181 taataattaa tatatattaa acaaagatat attaagatat tttagaacat aaaactgaat
      241 taaggcgcga gtactatttt gtattcgcgc cttgatggtt tgagttattt gagtaaaaac
      301 taactttaat aagaaatatg tatttattaa gttgatattt ttaatgtgtt tagagattat
      361 ttaacaatct ccaaatataa aactagaatt atttacataa aagttaaata gtttgtatag
      421 agttagattt atatagtata atacttagtg taaataatta agaagttatc aaacaggagg
      481 ataaaactgt gttagatata aattcttttt taaatggatt ttttaatata atattaagaa
      541 tgagtatata tgatttaatt gacatatcta tagtagcgta tattttttac aaaatattta
      601 tgtttataaa agatacaaga gcagaacaag tgtttaaagg tataatattt cttttattag
      661 caactcaact tagtaacacc tttaaattgc ataccgttta ttggatatct cttaaagctt
      721 tagattatgg agttatagct gctcttataa tttttcaacc agaatttaga gcaggacttg
      781 aacatatagg aagagcaaag tttaatttat ttggaaagaa tgtaaatact tctgaagaaa
      841 ctctcaatag aaatatagaa gaaattgtag aagctttata ttcactttct agacaaaaaa
      901 taggagctct tatcataatg gagagagaaa ctagaataag tgatataata aatacaggaa
      961 ctattattga tgctgaaatt tctaggcaac tattaataaa tatatttata ccaaatacac
     1021 cattacacga tggagctgtt gtaattagag attctaaggt aaaagcagct gcatgttttt
     1081 tgccattgac agaaagtaag gatttaagta aagatttggg cactagacat agagcaggaa
     1141 taggggtaag tgaggtgtct gactgtataa ctttgattgt atcagaggaa acaggtggcg
     1201 tatcaattgc taaagcaggt aagttatata gagatatttc tagagaaaga atgatgaata
     1261 tattacgtag taatttaaaa acaaatacag aaacaagaag ttttttcaaa ggtggtatat
     1321 ttaaatgata aatagactaa aaaataatac caaaataaaa ataatatcat ttttaagtgc
     1381 aattgcatta tggatgtatg ttatggctgt agttgaccct gaagaaacta aagggtttga
     1441 agatatacct gtatctataa gtaatatgag tgatttaaag gataaaaatc ttgttatata
     1501 tccagacgaa aaattaacag cagatgtata tataaaaggt agtttatcaa aggtacaaag
     1561 tgttaaaaaa gaagatatac atgtatatgg aaccattgaa gacccaatac aaggacagaa
     1621 ggaagtatat ttaaaagcaa atattcctca aggtgttact ctagaattta agcatgatgt
     1681 tttagtagta aatcttgaaa aaaacataaa agaaaaaaga gatataaagg tagaagtaag
     1741 tggaaattca aaaagaaata taaataaaat tgaacttagt aaggaatttg tcgatgtatc
     1801 aggtccaaga actctagtta aagaagtcaa aagtgtaaga gctaatctag atgttggtaa
     1861 tgagacagat gatttttcta aaaaattgca attaatacca ttaaatagtg atggaactaa
     1921 ggttgaagga gtaacattaa gttcatcctt tgtgactgca aagatagagc tattaaagga
     1981 aaaaattgtc ccaataagag taagtctaaa tgataataaa gataatgatg tactgaaaaa
     2041 ctataaatta agtaaggaac aagttacaat aaaaggaaag aaagaagata tagacaaaat
     2101 aagttatatt aagacaaaac ctattgactt agctgatttg gctaatggtg aagaaaaaga
     2161 agttagctta gaaattccag agggtataac tcttgaggat agctctataa caattaaatt
     2221 aaatgataca aaacaattga gtgcagaatt tttatataat gcgggagata ttgaattaag
     2281 aaatatccca acaaatatgg ataatccaaa tgtaatttct gatgtagata taaaagttgt
     2341 tgtagagtca acggaggatt tgagtaagtt aaataaaaaa gatatttcat tatatattga
     2401 tttatcacaa ggtcctgagg aagatagtaa atataaaata aaatatgaaa caacatatca
     2461 atttaaaaaa ataaatatag aacctaatat tgttgaagtg gaatgaacaa atatttcgaa
     2521 tatttagttg ttacaagatg cgacaaaaaa acctataaaa ttaagtttgt taattttatg
     2581 tttttgttgc atcttgttta ttttagttat attataaaat aaggacaatg agatatttgt
     2641 taaatctaaa aagcgctaga tatatgaaaa aagactgata aatagattta aataaatatt
     2701 ttgaattaaa tatttcaatt atctctagga aaacgttgct gaacgagtta taatttaatt
     2761 gagatataaa aagttctatt tcaaaaaatt caagatgagg tggaaatgca tgagaagttt
     2821 tgaagaagta attaagtttg caaaagaaag aggacctaaa actatatcag tagcatgttg
     2881 ccaagataaa gaagttttaa tggcagttga aatggctaga aaagaaaaaa tagcaaatgc
     2941 cattttagta ggagatatag aaaagactaa agaaattgca aaaagcatag acatggatat
     3001 cgaaaattat gaactgatag atataaaaga tttagcagaa gcatctctaa aatctgttga
     3061 attagtttca caaggaaaag ccgacatggt aatgaaaggc ttagtagaca catcaataat
     3121 actaaaagca gttttaaata aagaagtagg tcttagaact ggaaatgtat taagtcacgt
     3181 agcagtattt gatgtagagg gatatgatag attatttttc gtaactgacg cagctatgaa
     3241 cttagctcct gatacaaata ctaaaaagca aatcatagaa aatgcttgca cagtagcaca
     3301 ttcattagat ataagtgaac caaaagttgc tgcaatatgc gcaaaagaaa aagtaaatcc
     3361 aaaaatgaaa gatacagttg aagctaaaga actagaagaa atgtatgaaa gaggagaaat
     3421 caaaggttgt atggttggtg gaccttttgc aattgataat gcagtatctt tagaagcagc
     3481 taaacataaa ggtataaatc atcctgtagc aggaagagct gatatattat tagccccaga
     3541 tattgaaggt ggtaacatat tatataaagc tttggtattc ttctcaaaat caaaaaatgc
     3601 aggagttata gttggggcta aagcaccaat aatattaact tctagagcag acagtgaaga
     3661 aactaaacta aactcaatag ctttaggcgt tttaatggca gcaaaggcat aattttaagg
     3721 gggaaatagg caaatgagca aaatatttaa aatcttaaca ataaatcctg gttcgacatc
     3781 aactaaaata gctgtatttg ataatgagga tttagtattt gaaaaaactt taagacattc
     3841 ttcagaagaa ataggaaaat atgagaaggt gtctgaccaa tttgaatttc gtaaacaagt
     3901 aatagaagaa gctctaaaag aaggtggagt aaaaacatct gaattagatg ctgtagtagg
     3961 tagaggagga cttcttaaac ctataaaagg tggtacttat tcagtaagtg ctgctatgat
     4021 tgaagattta aaagtgggag ttttaggaga acacgcttca aacctaggtg gaataatagc
     4081 aaaacaaata ggtgaagaag taaatgttcc ttcatacata gtagaccctg ttgttgtaga
     4141 tgaattagaa gatgttgcta gaatttctgg tatgcctgaa ataagtagag caagtgtagt
     4201 acatgcttta aatcaaaagg caatagcaag aagatatgct agagaaataa acaagaaata
     4261 tgaagatata aatcttatag ttgcacacat gggtggagga gtttctgttg gagctcataa
     4321 aaatggtaaa atagtagatg ttgcaaacgc attagatgga gaaggacctt tctctccaga
     4381 aagaagtggt ggactaccag taggtgcatt agtaaaaatg tgctttagtg gaaaatatac
     4441 tcaagatgaa attaaaaaga aaataaaagg taatggcgga ttagttgcat acttaaacac
     4501 taatgatgct agagaagttg aagaaagaat tgaagctggt gatgaaaaag ctaaattagt
     4561 atatgaagct atggcatatc aaatctctaa agaaatagga gctagtgctg cagttcttaa
     4621 gggagatgta aaagcaatat tattaactgg tggaatcgca tattcaaaaa tgtttacaga
     4681 aatgattgca gatagagtta aatttatagc agatgtaaaa gtttatccag gtgaagatga
     4741 aatgattgca ttagctcaag gtggacttag agttttaact ggtgaagaag aggctcaagt
     4801 ttatgataac taatgataaa agaataagaa tcataactgg tcactatgga agtggtaaaa
     4861 gtgaatttgc gatgaactat gttgttaagt taagagatat ggtttctggc aaagttgcta
     4921 tagccgattt agatgtggta aatgtatact ttagaactag agaaaagaaa gagttaatga
     4981 aatccttagg tatacaacca atagatagct caattaatgc tccaacatta gatttaccag
     5041 cagtatcagc agaggtaatg tctcctatgg tagaccattc atacaattct gttatagatt
     5101 taggtggaga taatgtaggt gctagggtta taggaagatt tagtcatttg ttaaaagaag
     5161 gcgattatga tatgcttttt gtaataaatg caaataggga gaaaactcaa acttctgaag
     5221 aagttatcca atatataaaa gaaatagaaa aatcttctaa attaaaggta acaggtctaa
     5281 ttaataatac tcatcttatt aggtttacta ctattgatga tgttttaaga ggtcagaaag
     5341 ttgcaaaaga agtttctgaa aaatgtaata taccgataag atatgttgca tgtttagaaa
     5401 atcttgttga acagttacca aaagatttag aaggagaaat ttttcctata aaattatata
     5461 tgagagaaga ctggatgtag tttgagctca aatatataga aagtttaagg aggcttaggt
     5521 aaatggctaa aggaaaagta tcttttaatc aagaacgttg taaaggttgt ggattgtgtg
     5581 ttgaagcatg tccagtaaaa atcatacaat tagactcaaa tgtaataaac aaaaaagggt
     5641 ataacccagc aactgttttt gaaatggaaa aatgcatagg atgtgcaagt tgtgcaacta
     5701 tgtgtccaga tgtagttata acagtagaaa gagattaatt taattaatat ttaaggggga
     5761 attttaatgg ctaagatact tatgaaaggt aacgaagcat ttggaaaagc agctatagaa
     5821 gctggttgca aatatttctt cggttaccca ataactccac aaagtgaatt accagaatat
     5881 ttatcaagag agttacctaa aataggtgga gcgttcgttc aagctgaatc agaagtttct
     5941 gcgataaaca tggtttatgg tggagcaggt gcaggagcaa gagttatgac ttcttcatct
     6001 tcaccaggag ttgctttaaa gcaagaaggt ataacatatg cagtaggagc agaagttcct
     6061 tgtgttgtac ttaatgtaat gagaggtggt ccaggacttg gaagtataca accttcacaa
     6121 gctgactact ttatgtctac aagaggtgga ggaaatggag actacagaac tccagtattc
     6181 gcaccagcta cagttcaaga agctgttgat atgataatgg aagcttttga cgttgcagat
     6241 tattacagat caccagttat ggtagttgca gatggtatga taggtcaaat gatggagcca
     6301 gttgaattta gagctcctga gaagaaaaga gaattgcctc ctaaagattg ggcaactgtt
     6361 ggaactaaag gaaaaagaaa acctaatgta ataaactctc tatacttaga gccagaagta
     6421 ctagaagacc attgttggca tttacaagaa aaatttgatg caatggaaaa gaatgaagta
     6481 caatacgaaa tgtacaaaac agaagatgct gaatttgtat ttgcagctta tggaactact
     6541 tcaagagtag taaaaagcgc aattgatata ttaagagaag aaggaataaa agcaggtctt
     6601 ataagaccta aagtattatg gccattccca ttcgaagcat tcaaccaaat tcctaatgct
     6661 agaaacatat tgactgttga aatgagtatg ggacaaatgg tggaagatgt taaaatggca
     6721 gtagaaggta aattaccagt ttacttccat ggaagaccag gaggaatgac tccaacacca
     6781 gcagaaatag ttgaaaaagc taaaaaaatt attgccggag aattggttgc aggaggtgct
     6841 agataatggc tgttgtattt aagaaaactg aaggattaca agatactcaa actcactact
     6901 gtccaggatg tacacatggt ataatccata gattagttgg tgaagtatta gaagaactag
     6961 gagtattagg tgatgctgta ggtgttgttc cagttggatg ttcagtttta ggatataaat
     7021 attttaactg tgacactcaa gaagctgctc atggtagagc gccagctgct gctacaggaa
     7081 taaaaagagt tcatcctgaa aatacagtat tcacttatca aggtgatgga gacttagctt
     7141 caattggtac tgctgaaata gttcatgcag ctgctagagg agaaaaaata actactatat
     7201 ttgtaaacaa tacaacttat ggtatgactg gtggacaaat ggctccaact acattagttg
     7261 gacaaagagc tactactgct caatcaggaa gaaatgctga aactcaaggt tatccaataa
     7321 gagtaagtga aatgttagct acattaacag gagctgtatt tgtagaaaga gtagcagttg
     7381 atactccagc tcatgttaga caagctaaaa aagctataaa gaaagcattc caagttcaac
     7441 aagcaggatt aggatttgga atagtagaag tattatctac ttgtcctaca aactggggac
     7501 tagctccaaa cgatgcttta caatggttaa gagacaacat gataccatac tatccattag
     7561 gaaatttcaa aaatgttgaa gttgaggagg tgaagtaaat gtctacagct agagtaatat
     7621 gtgcaggctt cggaggccaa ggtgttatgt ctatgggaca attacttact tatgcaggaa
     7681 tgctagaagg aaaagaagtt tcttggttac catcttatgg accagaaatg cgtggaggta
     7741 cagcaaactg tgcagtaact gtatctaatg agccagttgg ttctccactt ataacagatg
     7801 atgcaactgc agctatatta ttaaatatac cagcatttga aaaattcaaa gatgatgtta
     7861 ttcctggtgg aaaaataata gtaaatagtt ctttaataaa agaaaaagtt gatagaacag
     7921 atgtagatgt ttattatata ccagcaaatg aattagcagc tgaattagga aatgataaag
     7981 ttgctaacat gataatgtta ggtgcttact taaaagtatc tgatactgta gatatagaat
     8041 ctgtactaga agcattcaaa aaagttttcg gacctagaaa agaaaaattc gttccattaa
     8101 acagagaagc tttacaaaaa ggtatggatg ctacatgttc taaagcaact aactaataaa
     8161 gaaaaagatg tcaaaaaaaa gacaatagaa ttatttctat tgtctttttt gctgataagt
     8221 attaaaatat ttttatgact gaaattaagc ctataatgat attttatgaa ctataaaaat
     8281 ataatacaaa ttacaagctt ttcaattatg aaaggagaat ctttagtgag aaagtatttt
     8341 ggaacagatg gagttagagg agtagcaaat acagaactaa catgtgattt agcatataaa
     8401 cttggtagag ctggtggatt tgtcttagct caaggagacc acagagtaaa agtagtagta
     8461 ggtaaggata ctagaatatc aggtgatatg ttggaagcat ctttaatagc aggacttatg
     8521 tctgttggat gtgatgttat tactgttgga ataataccaa cacctgcagt ggcatattta
     8581 actagaaaat atggtgctga ttgtggtgtg gttatatctg catctcacaa tcctgttgaa
     8641 tataatggaa tcaaattctt taataagaat ggatataaat tagatgatga aattgaattg
     8701 aaaatagaag aatatataga cgatatagac aaaattgatt gtcttccaat tggtgaaaat
     8761 gtaggaagaa aattacatga acattgtgca caaagagact atgtagacta cctaaaatca
     8821 ataataagca ctgactttaa gggattaaaa gtagttttgg actgtgctaa tggtgcgtca
     8881 tataaagtgg caccaatagt gtttgatgaa ctaggagcaa gtgtaatttc aataaatagt
     8941 tcaccagatg gaaataatat aaactataaa tgtggctcta cacatccaga acaacttcaa
     9001 agggctgttt tggaacataa tgctgactta ggtcttgcat atgatggaga tgctgataga
     9061 ttaattgcag taaatgaaaa gggtcaaatt gttgatggag accatataat gatattgagt
     9121 gccttaaatt taaagaaaaa taataaattg gcacaagata ctttggtagt tacagttatg
     9181 agtaatatag ggttgacaat agctgctaag gaaaatggaa taaatttatc tactacagca
     9241 gttggagaca gatatgtttt agaagatatg gttaaaaatg gatataatct tggtggagag
     9301 caatctggac atatgatatt tttagattac aacactactg gagatggagt gcttagttct
     9361 ctaatacttg caaatataat cttgcaagaa aagaaacctt tatcagaaat tgcatcaata
     9421 atgagccaat acccacaagt attagtaaat gcaacaataa aaaatgaaaa taagaacaaa
     9481 tatatggaat atccagaaat aaaaactgag atagaaagaa tagaatctat tttagacgga
     9541 aatggtagag ttttgataag accatcagga actgagccat tagttagagt tatgttagag
     9601 ggtaaagagg aaggtcaaat aaaagaatta gcaacaaact tagctaatct tatacaagaa
     9661 aaattatcgt aatttaaagg gtattctcag atataaatac tgaggacacc tttctaagcg
     9721 ccagaactta gttatagaca taattattga gtaatatagg aactaagttg acgaggtcag
     9781 agttaatcga attgtcggcg gatgctctgc ggtgtgttta ccatcgaaag acttctttaa
     9841 ataataggag caatcctatg gacaaaggga agccaaagta aacttatatg acctaactct
     9901 taaaatatat aaataatatt tttaggagga cacaaataat gtgtggaata gttggatatt
     9961 taggaagtag aaaggcagca gaagttatag tagaagggct ttctaagcta gagtacagag
    10021 gttatgactc agcaggagta gcagttaata gttctaatga aaaagaactt aatattagaa
    10081 agtttaaagg tagactttct gtacttgcag aagacttaga aaagaatcct attgatggaa
    10141 atttaggtat agggcatacg agatgggcaa ctcatggaga accatctgat gtaaactcac
    10201 atcctcattt taatcaagcc aaaacaatag cagttgttca taatgggata atagaaaact
    10261 atatggaaat aaaagaagaa ttaatttctg aaggtgttaa gtttgaatct caaacagata
    10321 cagaggtaat agcacattta gtagataaat actatgaggg taatttatta gatgctgtat
    10381 acaaaactat atcaaaatta agaggagctt atgcattagg tgttatatgt aaagaacatg
    10441 gaaatgaatt agttgcagta agaaaagata gtcctcttgt agttggtgtt ggtgaaggtg
    10501 aaaactttat agcatcagat atacctgcat tattaaaata tactagagat gtttatttct
    10561 tagaaaatgg agaagtagtt catctaaaag acgaaaatgt aacagtttat gactctaata
    10621 gaaatcttgt tgaaaaagaa gtatttcatg taacttggga tgtagaagct gcgtctaaag
    10681 gtggatatga ctactttatg tcaaaagaaa ttcatgagca accaacagga gtaagagaaa
    10741 ctttagaaag aagacttgat gataatggaa atataattct agacagtata aatatttcta
    10801 aagaagactt agaaaaaata aataaagttt atatagtggc ttgtggaaca gcatataatg
    10861 ctggattact tggtaagtat gccatagaaa aatttgtaaa tataccagta ataactgata
    10921 tagcatcaga atttagatat agcgacccat ttgtagatga aaattcatta gtaatattag
    10981 ttagtcaatc tggtgagaca gcagatactc tagcagtatt aagagattca aaggctaaag
    11041 gagcaagaat actttcaata actaacgttg taggttcatc tatagcaaga gaatcagatg
    11101 atgtattcta tacttgggca ggacctgaag tagcagttgc atcaactaag gcatatacta
    11161 ctcaaataac ttctttatac atgatagctt tagactttgc tatcaaaaaa ggaacaataa
    11221 ctagagaatt ttatgatagt atgatttcta agatgaaaga aataccatca aaaatacaag
    11281 aaatactaga caatgaagaa tatataaaag aagttgctaa aacagttgtt agttctgaac
    11341 atgcattcta tttaggaaga ggtatagact atagcttagc aatggaaggt tcattgaaat
    11401 taaaagagat ttcatatata catgcagaag cttttgcagc aggagagcta aagcatggaa
    11461 caatagcttt aatagaaaaa ggaactcctg taattgccat agctactcaa gaaaagttat
    11521 ttgaaaaaat ggtttctaat atggaagaag taagagcaag aggagcttat gtagtagcaa
    11581 tagctcaaag ccataataag gatgttgaga aagctgctga taaaataatc tatataccaa
    11641 attcagatga tattttatca ccaatattag cagttgtacc aatgcaatta cttgcttatc
    11701 atgtctctgt actaagagga tgcgatgtag ataagccaag aaatctagct aaatcagtaa
    11761 cagttgaata aaaacagatt aaaaattaaa tagataatat ttattgaaaa gaactcatcc
    11821 tgttatttgt attttatagg atgagttttt taaataaata ttaaatttgt tttgtttttg
    11881 ctataataaa ttttataaaa agattgatat taaatcatct gtaaaatgat aaatatatag
    11941 accataccag gtatagggaa cgataaatat agttcatata aaatatagtg agtacttaat
    12001 aaacatatag tcaatatcag ataaaaaaaa taatagctta tgagaaaaat taaaatacaa
    12061 ggtattagta accttgtgat aagataaagt gagtagttta aaataagaat ataaggaact
    12121 atttataaaa gaatataatg aagctaataa agtataagaa aattacttta ttttagtaaa
    12181 ataatttcaa ataaaaattt tttaaattgg gagtataaat tatgacaaat gattttaaaa
    12241 aagaaaagaa agaagattat tttgttaatc ttaaggcaat atctactgaa gttttacaag
    12301 aaaaagtgca agataacgct tgggttatag ttgatactcg cttaaatgat gcttataatg
    12361 gctggaagtt agatggagta aaaagaggtg gacatataaa aggagcagta gatttttctg
    12421 ctaattggtt aagtgtatac tcagatagaa aagatgaagt gctagaacaa gcattgaaaa
    12481 ctaaaagaat tgatttagat aaaaatatag tattatatga tgcaaatgga aaagatgctt
    12541 tggttgtagc agattattta tctaaaaaag gatataaata tctatataaa tatgatatta
    12601 aacaatgggc agacaacgaa aatcttccaa tggaaagata taaaaattat caaatgatag
    12661 ttcctgcttt tataataaaa gatatattag atggaaaaat tcctgagaca tttgaagatt
    12721 caaagaatat aaaaatgatt gaagcgagtt ggggagaaga atcgtataca aaagggcata
    12781 tacctacaag tgttcatgta aataccgata ttatagaacc tcctccaaca tggatgcttg
    12841 ataatgatga taacttaatt aagtttgctt tagactatgg attaacaaag gatgatactg
    12901 taatagtttc aagtagtaca cctatggcat cttatagact agctgttata ttgagatata
    12961 taggagtaaa agatgtaaga gttttgaatg gtggaacaaa ctcatggtta tcagcaggat
    13021 atgaattaga atttactagt aatccaaaac attcatgtac taattttgga gcagatatac
    13081 cagttaactc acaattagtt gttactacct ctgaattaag acaaaaacta aaagaaaaaa
    13141 ataagtttat actagtagat aaccgtactt gggatgaaca tataggaaaa gtatctggat
    13201 atacatatta tgataaaaaa gggcgtatac caggagcttt atatggacat tcaggtagtg
    13261 atagtgtttc actagaagaa tatcgtaata ttgataatac aatgagaaat aaatatgaga
    13321 ttttagaaat gtgggacaag gaaaatatag atgtgaatag acagcttata tttatgtgtg
    13381 gaagtggatg gcgtgctgct gaagtattaa catacgcaaa tgtcatagga gtggaaaata
    13441 cctcccttta tagtgatgga tggatgggct ggagtcttga taattcaaat ttaattgaag
    13501 tgggagaaca caaataaagt ttttgaaagt acgatagagt tgtttaaaac aaaggtgttt
    13561 ttgatttatt gggaagagtg tgtaaattgt gttttgggaa gtgtacttag gtatgttcat
    13621 ttggagctat ggaaattgat aaaaaagtat tatctgttta ttttgctggt aaaatgtgaa
    13681 tagattatag attatagatt atagattata gatttaagta tagactaagt aatttatatt
    13741 acagaagaga atatataaga actagtgcaa gatgtatttg atacatataa aaaattggca
    13801 aatccagaat aaggatagag aaaattgaga ttgaagagtt tgaaagagac ttattagatg
    13861 aaaaatacaa agatgtataa aaatacatat tgagaaagta tatctctctt aaatttgtta
    13921 ataattctat tatacagaaa aacatattta agggggattt tttactatga aaagagtaaa
    13981 attagctata agctttttta tattaatgat tatagggatg acaatatctt atgcagatac
    14041 aagtataaat actaaataca atagattaag taaagctctt gaaatttcag aagcaaattt
    14101 taaattttat aatgttaaaa ttaattctgt tataaatcat aatttatcaa ataaagaatt
    14161 tcataatatt tttatagaaa taattaacaa tctaaagttg gataaaaaag gaataaattg
    14221 gaatcaaaaa gggtctggcg ataaaataca attatgtgct actataaaag gtagcgataa
    14281 agaaatttct ataaaaggaa taaataaaaa taacaaagaa agttacatag tagttgacat
    14341 attggataac aaagtatata aacataaaga ggttatttat agtaacgtag aaaatgtgtt
    14401 aaataaatat tcatctcaag tagatgtgtc tgcttgtatt gttggagaat acacaaaaag
    14461 gttacaaaac aacaaatatg atgatatttt acaaaaaata ttatataata tgagtgctaa
    14521 gcaaatagat gaagtgaaag acgtaaactt tttatctata acagcatata gtaaagtatt
    14581 aaatgattat gaattagaat atttagggga aaaaattaat ttaaatatag gaattaaata
    14641 taatgaggac gacggaagaa cactcatata tattgccaca ccaattataa aattagatta
    14701 ttaattatga ggagatcaaa aaatatggct aagataatag taaaaaagag taatccactt
    14761 aaaggtagtg ttaaaataga tggtgcaaaa aatgcagttt taccaataat agcagcgact
    14821 ttattagcaa acgggaaatc aacattaaat ggagtaccaa atttaagaga tgttcatgtt
    14881 atatcagatt tattaagaca tgtaggggca gaagttgaat ataaagaaaa tacacttact
    14941 gttgatgcaa gtaatataaa aacttgtgaa gctccatatg agcttgtaag aaagatgaga
    15001 gcttcttttt tggttatggg gccattgctt gctagattta atagtacgaa aatttctatg
    15061 ccaggaggat gcgctatagg aacaagacct atagatttac atttaaaagg atttaaagcc
    15121 ttaggtgcaa agatagaaat ggaccatggt tttgttgaag cagcaacaga aaaattagta
    15181 ggaaataaat tatatttaga tttcccatct gttggagcaa ctgaaaacat aatgatggca
    15241 gcttctttag cagaagggac tacaataata gagaatgcag cagaagagcc agaaatagta
    15301 gatttagcaa acttcttgaa cgaaatggga gcagatgtta aaggtgcagg tactaatact
    15361 ataaaaataa aaggtgttaa agagttaaaa ggagcagaac ataatgtaat tccagacaga
    15421 atagaagcag caacttatat ggttgcagca gctatgacaa aaggtgatat aactgttgaa
    15481 aatgttttaa tggagcattt aaaaccagtt gtagctaaat taagagaagc tggttgtgaa
    15541 ataacagaaa tggacaattc tgtaagagtt gtaggtccaa aagtattaaa accaatagat
    15601 ataaaaactt taccacatcc aggatttcct acagatgttc aagcacaatt tatggctatg
    15661 cttactgtag ctaatggaac tggtgtagtt atagaaactg tttttgaaaa cagatttatg
    15721 catgttgctg aatttaatag aatgggtgct aatataaaaa tagatggaag aagtgcggtt
    15781 gttaatggtg tagatgagct acatggtgca gcagtaaacg ctactgattt aagagcaggg
    15841 gcagccctta ttttatgcgg tcttatagca gaaggagaaa ctcaaatagg agaaatatac
    15901 catatccaaa gaggctatgt tgatatagat aagaagataa cagcattagg tggtcaaatc
    15961 gaaatagtag aagattaata attggtttat agaagaaagt tataaattaa tagggttgtt
    16021 catatactta aatacaaggt tttaaaagct aaggaggaga gtatatgaag aacccattgg
    16081 ttgttttgtt aggatttgtg acttgctctg tgttagttcc atcacttata actttagttt
    16141 cttataaaaa tgtagaatta actgaaaaag cagagagccc agtatctata aacaaaacta
    16201 ttaaaaaaag tgatattgaa gacaaaggta ataaagaaga aaaaaataca gtgaactatg
    16261 aaactgtaaa taaaaaagca ccaattatca atgtatataa tcatataaca gggaaaaccg
    16321 aaaagatgga catggagaac tatttatgtg gagtactagc tggtgagatg tcttcagagt
    16381 ttgatataga agccttaaaa gctcaatctg tagcagctag aacatatgta gtatataaac
    16441 aggaacatgg taaatctagt aaacataaaa atgcagtagt atgtactgat tataaacatt
    16501 gtcaagaata taagagttat gatactctta aaaaattaaa tggtgaagaa tggattaaga
    16561 ataaatattc caaaatccaa gaagcagtta gaggaactaa aggtcaaata ataacttata
    16621 acgataaggc gatacttcct ctttattttt ctacatcttc aggaaaaaca gaaaatagtg
    16681 aagaagtatt ttctgcaaaa tatccttatt taaagtctgt ggaaagtcct tatgataagt
    16741 attcccctaa atttgcgtca acacttaaaa taagcaacac agattttgtt aaaagtctta
    16801 gaagggcata tagcactatt gtgatagatg tgaataattt aagtaaacaa gtttcaataa
    16861 ctaaaagaag tgatgccgga actgtagaaa aaataaaatt aggaaataaa gaattgactg
    16921 gaaaagacat tagaactgtt tttaaattga actctgcaaa ctttgacata aaatttggag
    16981 aaggatacat agatttcgtg gtaaaaggat atggtcatgg tgttggtatg agccagtggg
    17041 gagcagaagg aatggcagaa gagggctata aatattatga tatattatca cattactata
    17101 cagatacaaa aataaaagat atatactaat aattgtataa aatgccaaaa acacgtcaat
    17161 aatccaaatg tagaaatata tttggaggtg caaaatgaag aaaaagctgt tagaaaaaga
    17221 tggtttttat ttatctttgt ttgtatgtgt ttgtttatta gcagtaggtg gagtttggtt
    17281 tacaaataat aatgtagata aattggcttc taataaagga attatggaaa atgctaataa
    17341 agatagtgaa gaggaaatac atttaattga aaaagacaag aaagatgcta tccctactgc
    17401 aacggattca aaacaaaact tagaaaaagc taagtcaaaa gaggaaaata aatcgagtac
    17461 aacaaaatta aattacattg gagataaagt aataagagga tattcagaaa aagaacctag
    17521 ttattctaaa acacttgatg tttgggaaac tcataaaggt gtagatatta gttgtactaa
    17581 aggcaaagaa gtaaaatctc tattaaacgg aatagtagta gatgtattta atgatgaaga
    17641 atatgggcaa tcagtcaaaa taaaaagtga caataacatt gtagtagtat attcaaatct
    17701 agataaaaat gttagtgtta agaaagaaca gaaggtaaca gaaggacaat ctctaggtac
    17761 tgtaggtagt acttctcaaa tagaaagtga agaaggtata catgttcatt tagaggcgta
    17821 tagtggagaa aaatctatag acccaatgag tctaattaag taaaagtata acaagtttaa
    17881 tcatatttgg ttaagcttgt tatatttttt ggtgttttta catatatatc attaaagcta
    17941 ttatgtgagg ggggtaatct ttgagatctc atatagagga aagggccata gtggtagcaa
    18001 aatatatact tgaaaaaaac actacagtaa gacaaaccgc caagacattt ggagtaagta
    18061 aaagtacaat tcataaggat gttacagaaa gattgaagga aataaatcca tcccttgcca
    18121 aagaagttaa aaatgtgctg gacaaaaata aatcagagag acatattaga gggggattag
    18181 caacgaaatt aaaatatgag aaggaacata aaaaaatgta ggttaaaaac ctactttttt
    18241 tatttaatta aattataaaa agaaatatat taagtaatta taaatagcaa aataataata
    18301 ctctcaaaag atttattttc tcttgattaa agcgagattt ttgtatatgc tttattatag
    18361 gagtattaaa ataggcttat aatataatct aatattataa ataaaggagt tgaaaaaatg
    18421 gctggagctg atataggcat agatttaggt actgcaaatg tgcttgttta tgttagtggt
    18481 aagggaatcg ttttagaaga accgtctgtt gtggcgatag acaacagaac agattctgtt
    18541 ttagctgtgg gagaagaagc taaaaagatg atagggagaa caccaggtaa tatagtcgct
    18601 ataagacctt taagagatgg tgtaatttca gattatgatg taactgagaa gatgttaaaa
    18661 tcttttatag ataagatagt tgataaaaag ggatttggaa gattttttat gccaagaatt
    18721 atggtatgcg taccaacagg tgtcacagaa gtggaaaaga gagcagtaga agatgctacg
    18781 agacaagctg gagctagaga agtatatata atagaagaac caatagcagc agccatagga
    18841 gctggtgtag acatatcaca gcctaatggt aatatgatta tagatattgg tggaggtact
    18901 gtagatatag ctgtaatatc tctaggagga gctgttgtaa gtgaatctat aaaggttggt
    18961 ggagatagat ttgatgatgc tatagttaaa tatatgaaaa aacaacacaa tcttcttata
    19021 ggagaaagaa cagcagagaa aatcaagttt gaaataggtt ctgcatttaa gagagaagaa
    19081 gaaaaatata tgaagataac aggaagaaat ttaattacag gacttcctag ttctataact
    19141 ataaattcaa cagaaatgtt ggaagcactt agagaatgtg ttgagcaaat agtagttgct
    19201 actcatgctg ttttggaaaa aacgcctcct gagttagcag cagatatagg agatgttggt
    19261 ataataatga ctggaggagg ttcattatta tatggattag ataaaataat agaacagaga
    19321 acaggaataa ctgttacaat agcagatgaa ccattatctt gtgttgcaaa aggtacagga
    19381 atagctttag gttcaataga cctacttgaa actggcgggt ctttcaaaag aaaataatta
    19441 ggagagttta gaatgttaaa tatagatgaa ataaaaaaat taataccaca tagatatcct
    19501 tttttattag tggacaagat tacagagcta gaagttggga aaagagctgt tggtataaag
    19561 aatgtaacag ttaatgaacc atttttccaa ggtcactttc cagaatatcc tttaatgcca
    19621 ggagtattaa tagtagaagc tttagcacaa gtgtgtggag tggcaatgat gtctgtagag
    19681 gaaaacaaag gaaaactagg tgtatttgca ggtatagata aagttagaat caagagagaa
    19741 gtaagaccag gagatacatt aactatggaa gttgaaatga caactcttag aagaaatata
    19801 gccaaggcag atgctaaggc atatgttggt gaagaacttg tctgtaaagg ggaattaatg
    19861 tttgccttag tagaaaaata aaaacttgaa atataaaagt aaaaaggtag ataatttgga
    19921 gtatttaatc agatactcta aaatatctac ctttttatat aaagaatttt atcatttagt
    19981 ttatatttag attaggtgga tactaataaa gccaaggcta ttgtttcagc taagtctaat
    20041 ataaagctaa gacgtatatt attaaatgaa agtttattat tttcatcaat tttatctaca
    20101 attcctacta tagaataatt tcctatttga ggtagttttt tgccaacacc ttttccgggc
    20161 aggataggtc cttctctaat ctgtatatta cctatgttac tctgcgaccc caagcaagca
    20221 tctatagcca agaagttacc ttgtatatgt gtatttttta tagtatctaa agactcatat
    20281 atatttaagg cgtgaatagg gttatctagt gtaccgtaaa cggggtattt aaagttactg
    20341 ttcttaagta ttgttccaac taaagggcct aaagtatcac ctatagccct gtctgttcct
    20401 atgcaaacaa caatcgtatt ttcattaatt atagttttta gaactgaact tagcatctgt
    20461 ataacatcat catcgctgta atttacttta gctaaataca ataggaacac ccccttcaaa
    20521 aaaatatatg aataaaaagc attaaaaaga cctaacttta aatttcagat agatagaaaa
    20581 ttttatttgt cttacaaaaa aaggaataga aaaaatatga ctaaaaatat tgacaaaatt
    20641 ttaaaaaaat agtaagctag taaagtaaaa caaaacttaa atcttgatta ccttatcaag
    20701 agaggctgag ggataggccc tgtgaagccc agcaaccacc caaatgggaa aaggtgctaa
    20761 ttcctacaag acaaaagtct tgaaagatga ggtcagttaa ttagtatgta attacgatta
    20821 ttaacctctt cttataaagg agaggttttt tattttgagt aaatttctaa aattaaaaat
    20881 aaggtaaaac ctataataaa aactaagaat aaataagggg gaataaaaat ggcaagacat
    20941 ttatttacgt cagaatcagt tacagaagga catcctgata agatatgtga ccaaatatca
    21001 gattcaatat tagatgcatt attagaaaaa gacccacaat ctagagtagc ttgtgaaact
    21061 acagttacta ctggtttagt tttagtagca ggagaaataa gtacatctgc ttatgtagac
    21121 attcctaaat tagtaagaga aacagtaaga gaaattggat atacaagagc aaaatatgga
    21181 tttgattgtg atacttgcgc agttataact tctattgatg aacaatctgg agatatagct
    21241 atgggagttg atgaaggcct agaaagtaag actggagaag agatagaaga agaaatagaa
    21301 aaagttggag ctggagacca aggtataatg tttggatttg catgtaatga gactccagaa
    21361 ttaatgcctc ttccaatatc tttggcacac aaattatcaa gaagacttac agaggttaga
    21421 aagactggtc tagttgatta tttaagacca gatggaaaaa ctcaagttac tgttgaatat
    21481 gaaggaagta aagctgtaag agtacataca gttcttatat cagctcaaca ttgtgaaaca
    21541 gtatcaaatg ataaaataag agaagattta attaatcatg ttattaaaga agttatacca
    21601 gcagaattac ttgatgaaga tactaaaatt tatataaatc caacaggaag attcgttata
    21661 ggaggaccac aaggagatac aggtcttaca ggaagaaaaa taataataga tacttatggt
    21721 ggatattcta gacatggtgg aggagctttc tcaggaaaag accctacaaa ggttgataga
    21781 tctgctgctt atgcagctag atatgttgca aaaaatattg ttgcggctgg tcttgcagac
    21841 aaatgtgaga tagaacttgc atatgctata ggtatagcta gaccattatc tatatttata
    21901 gatacttttg ggacaggcaa agtttcagaa gaaaaacttg ttgaattagt aaataaacac
    21961 tttgatttaa gaccaggagc cataataaga gacttagatt taagaaagcc tctttataaa
    22021 aaggttgctg cttatggtca ctttggtaga actgatatag atttaccttg ggaaagaact
    22081 gacaaggttg agcagttaag aaaagatgct ttaggtgaat aatttttaac taaaaagctg
    22141 ttagaatgta attttacgat tactttctaa cagctttttt attatgagtt aaatattttt
    22201 aataaatgtt aatttatcat catataatga ataaacagta tttaatctgg tcaatacatt
    22261 ttatataata ctatactaaa tttatgcata ttatgggata tatgttaaag gatggttaag
    22321 aatgtgttaa ctaatgttaa ataattaaaa atatattgaa atatcataat aatgagtcta
    22381 atataatctt aataaactta cactttaaaa ataattttaa ttggggggag tagtgtatgt
    22441 caggcaaagt aaatattaaa acggttataa gttttgctgg agcttatgtt gctactgtaa
    22501 taggttctgg atttgcaact ggtcaagaaa tactgcaatt ttttacattt tatggatatg
    22561 ctggtattat aggtggaata atctctatgg tactattttc atggtttgga gcagaggtta
    22621 tagataaggg aagagaattg aaacttaagg agccaataaa aatttatcaa gtatattgtg
    22681 gaaaatatct tggaacattt tttgaatggt ttggtccatt gtttttattt ggaacattag
    22741 taataatgat tgcaggagca ggagctacat tatcagagta ctatggatta aatccatatg
    22801 taggtaggat tggtatgaca atcgtttccc taataactgt ttcattaggt ttgacaaggc
    22861 tttctaaaat actaggtaat ataggaccta taataataat atttacactt ttagtaggag
    22921 caattagttt atttagcaat attgatgctt tatcaaatgc tggaaatatg gttgatagtt
    22981 taaatattaa aactgctaca tcaaatggat atttctcagg agtactttat acttgttata
    23041 atgtaataat agtaataaca ttcttgacag gaatgggagc ttctgcagta aataagaaag
    23101 atgctgtgtg gggtggtata gtaggtggag tagcacttat ggcagcagct ataatgatga
    23161 atttggcatt actttcagac ataggaaata tatacacaaa agagatacca gcactttact
    23221 tagctgataa gatatctcca ataataggta ttttattctc agtagtactt ttactaggaa
    23281 tatacactac agctgtacca ttgttatggt cagtaactaa ccgatttgta gaagatgacc
    23341 accctaaatt taaaattata acaatagtag taagtatatt agcttgtata ggaggtctac
    23401 tgccatttga taagttagta ggtacactat atccatatac aggttacatg ggaattttaa
    23461 ttttactatg tatgctttat agaagaatta ctaaaactga ggggtacaag gagaataaat
    23521 ctgaaataag ctagtactgt aatatactta tgaaatttaa tttatactct aaatatggag
    23581 taattaaaat aaaatagcta tttaaatatg gtacctgtat ttcatggtac catattttat
    23641 ggatgcagat tttttttgtt tctgataaaa tgtataatag ttggaaatta cgttaagtat
    23701 aaaaatataa aacttttcta cgattatcat agttttaata tataaaatta tactaacatt
    23761 taaatggagc gattattagt atttttagga gataaaaatg gaaaaattag aaggaatgat
    23821 aagtgaaata gtctttaaaa acgaagataa tggttataca atagctcatc tggtaaatga
    23881 aaatgatgaa attgtcgttg taggatgtat gcctactttg gcaattggag aaagcataga
    23941 agttgaagga aagtgggtaa accataagat ttatgggact caatttgaag tgaatagttt
    24001 tatgccagta acaccatcat ccttagaagg aatttatgtt tacttatctt ctggaatgat
    24061 acatggtatt ggtgaaaaaa tggctaaaag aataattgat aagtttggtg tagatacgtt
    24121 agaggtgata caaaattcac cagaaaaact acaagaagta gaaggtatag ggagtaaaaa
    24181 agtaaaacaa atagtaaaaa gttatgaaga agacagagaa ttgagaaata taataataca
    24241 gctatctcct tttggaataa caccaaatta ctgcctaaaa atatataaaa aatataaaag
    24301 cagtgcaata gaagttataa ataaaaaccc ataccaactt gctgaagata taagaggaat
    24361 tggatttaaa gtagctgata gtatagcaaa taaaattggt atagataaaa attctaagga
    24421 tagaatttgt cagggaatct tatacacttt aaataaatct ttaagtaatg ggcatacata
    24481 tttaccagag catgttctaa tacaagattc ggaaaaatta ttggagctaa atggagaaat
    24541 aataaaagag tgtgtaatga tgctagttta taatcaaaaa atacatgtag agaaagtaaa
    24601 caatgaaaat cttatatacc ttatgccata ctatttagct gaaaatggag tatgtagtca
    24661 aatagtaaaa ttatcacagt atgaatttga agacttaaaa atagatatag ataacgagat
    24721 taatgtttta gaagaagata agaaaattaa gttagcagaa aaacaaattt tagcagtcaa
    24781 agaatctgtt aatagtggcg tattaattat aaccggtggt ccaggaactg gtaaaacaac
    24841 taccattaat gctatcatag atatatttga aaataatggt aaaagcgtga ctttggcagc
    24901 tccaacggga agagcagcaa agagaatgag tgaaacttct aataaagaag caaaaactat
    24961 acataggttg ctagaaatgg gattttcaac agatgatgat ttaacttttt ttaaggatga
    25021 agaagaccca ataaattcag atgtaataat tgttgatgag gtatcaatgg tagacattat
    25081 cttaatgtac aatttactta gagctataaa gttaggaaca agagtaattt tagttggaga
    25141 tagtgaccaa ttaccttctg taggtgctgg aaatgtactt aaggatatga ttaattctaa
    25201 tattataaat gtagttaaat taaatgaaat atttagacaa gcgcaagaaa gtatgataat
    25261 tgtaaatgcc cataagataa acaatggaga accactttat ctaaatacaa aaggtaaaga
    25321 tttctttttt ataagaaaat caactaatga agaaatttta aatgaaataa taggattggt
    25381 taatgaaaga ttacctaaat tttataaagt tgataaatta aaagatatac aagttttatc
    25441 ctcaatgaga aaaggtgaat tgggagttac taatctcaat atagagttac agaagtatct
    25501 taacaaaaaa gaaaaattta aagttgaaga aagtttttct aaaagattat ttagagttgg
    25561 cgataaggta atgcaagtaa aaaataatta tacaaaaaaa tgggaaacag aagaccaaaa
    25621 agaaagtgga gaaggtatct ataatggaga tataggatat gtttatcaca tagataaaga
    25681 taaaaaaact atttatatat tatttgacca gactaaaatt gtatcatacc tatatgatga
    25741 attagatgaa attgaccata gtttttgtac aacaatacat aaaagccaag gaagtgaatt
    25801 tccagttgta gtattgccaa tagcttgggc tcctcctatg ttacttagcc gtaatttatt
    25861 atatacagca gtaacaagag caaaaaaatt agttgtatta gttggagatg tgaaatatct
    25921 tgaatatatg ataaaaaata atagagttaa tcaaaggtat tcaaatttag gatataagct
    25981 aaataaattt aagcaagaag ggctgttgat agaataaata tgaaaatatg caatgataat
    26041 tttattaaga attttaaaaa tatgattaat aaatgtttgg attttatata tcctgagaat
    26101 ataagttgca taatttgtga taaatccata aagaaaacaa atacttattc aatatgtaaa
    26161 agttgtttta aagaaatgaa ttttatacaa gatggttgca tgaagtgtgg aaaaccaata
    26221 atacgtcatt caattgaaaa agaatttatt gaagaatgta gttattgttt taataaagac
    26281 ttttattttg ataaatcaat atcttgtatt gaatacaatg atgtgacaaa aaagatagtg
    26341 ctagggctga agtataacca aaaaaccttt atggctaagt atatagctca aataatgaaa
    26401 gaaaaattat atttagaaaa cataaaattt gattatatat tatttgttcc acttcataaa
    26461 aaaaggctaa ataaaagagg ttttaatcag gcacaaaaaa tagcatttaa tttaagtaaa
    26521 atagtaaata ttccattatt ggattgtata agtagaaaaa aatatacaag aatgctatat
    26581 aaattgaata agaaagaacg aaaagaagaa ttaaaaaatg tatttgttgt taaagacaat
    26641 gttaaattaa taaataataa aaatatactt ttaatagatg atatatttac tactggtcta
    26701 acaacaaacg aaatttctaa acttttaaag ttatcgggtg ctaataaggt ttttgtattg
    26761 actctactta caaaagcaaa tgataattat attatggaat aatcttatat attgacaatt
    26821 ttaatttaaa atattaaaat gaataagtga tatttaggtc tgtggttgaa agtccaagcc
    26881 agtcgcaggc aaagggatcc acgtaagtta acactaaaaa ttgggttaat gatcatggtg
    26941 cggcttagaa gtaagaccta ccgataataa aatcgagaga gagtagtagt gcggcgaacc
    27001 caagcgaacc ctccagtagg cgagtgtggg gtcaaaaacc aggtcagcta aatatcgtat
    27061 taaaagggag ctagcatacc tttatgtata gctctttttt atatataata aatttaggtt
    27121 gagtcataaa gaggtgatat gtttgaataa aagacaggaa aacttattaa caacgttgct
    27181 attaaaagaa gagtttataa atgttaataa gattgccaat gattttgagt gttcagaaag
    27241 gacaataaga aatgattgca aatatattga tgaatggtta tctacttttt gtaatgctta
    27301 tatcaaaaga aaacctaata ttggaattaa atttagtgga gatggaacag acaaaatact
    27361 ggtaaatcag aagttgcgag gtgaagttaa aagagtaaat agtgagcttc ataagcaagt
    27421 tgagatatta aatttactac tttgtaataa taaaaaagtt actttaaatg atttatctaa
    27481 taagttgtac ataaataaaa atattgtcag agaagaaata agtaaattat cagtggcatt
    27541 aaataattat aatttaaatg tctcgactaa aaaaggcagt ggtatatata ttggagggga
    27601 tgagaaagac ataagaataa tgttagtatc attcttattt aaatacatag ataaatacag
    27661 attaaacata gatgaaatag agtacttcca tatagtagat ataataattg taaaaaacat
    27721 aatgtttact atagaggagg atatggatat aagacttaca gatatatcat tcaagcaatt
    27781 gatgctattt ttacttataa atataatacg aataaggcta ggaaattctt taaaagtatc
    27841 aaatactaag aaagagctta agctaaaaat gctgatatta gatatagaag atgaattgaa
    27901 atctaattta tccatagtat taaattatga agaaaaattg tttatagagt ctttaatatt
    27961 gggtatgaac aaacaaataa atagtagtaa tattaagaaa tctttatatc taaatgaaga
    28021 attagcaagt tatacaaaaa aaattataaa tttagtatca gaagaatccg gaataaattt
    28081 taataatgat atattacttt atgaacaatt aatatgtcat ttgaatgtta caatgcacca
    28141 aatgaaaagc aatgtttact tagaaaatcc acttttagat aacataaaaa ctaaattttg
    28201 ttttttattt acagtaatat caagttcaat agaaattgaa ttaaaaggaa aacaaataac
    28261 agaagatgaa attggttatc ttacacttca ctttcaaacc tctctagaaa gaaaatacag
    28321 caaaagggaa tcaaataaaa aggcaagtat tgtatgtcca tttagttttg gagtaagcat
    28381 gctattaaaa gtaaagatag aaaagagatt tgataacata aaaattattg aaacattaag
    28441 agaggaagat ttaaaaagag ataacttcaa taagactata gattttataa ttgcatttca
    28501 agagtatgaa aatatccaaa aaccaatttt tataacaaca ccattattta ctgatgagga
    28561 tgagaataaa cttaaagagt tcacaaacaa aataaaagaa aaagacactt catataaact
    28621 tatgaacaga ttaatgatgt ctgattattt aataagagag ttagaacatg atgatattta
    28681 tgaagctata acatatttaa ctaatttatt aattgaaaaa cattatgcag aaaaagagta
    28741 tttacaatct attataacaa gagaaaagtc atatccaacc aatgttggaa aaggaatatt
    28801 attccctcat ggagatatga aatatataaa acaatctgtt ttatgttttg caaggttaaa
    28861 aacaccaata agaattagaa atggaaagga tataaagatt atacttttgt tagcatataa
    28921 aaaagatgat gatagaaaag tatttagaga attatttaaa gaaatatcta atttaacaga
    28981 ggatgaaaat atgctagata ttctaagtaa atgtgacatt gaaaaagtaa aagatattct
    29041 tatataaaat tcatagtgaa ctatggataa gcacaaaatt ttccatgtga tggaaaaagt
    29101 tgtgcatgtt tttttgtttt tataagatat tatatgtttg taaggtataa ataatctaaa
    29161 agtagagaag caacttagga ggaaaaaatg gaagaaatag ttttaaagat tataattcat
    29221 gctggaaatg caaaatctat gctttatgaa gcacttgact atgcaaagga aaacgatttt
    29281 aaaaaagcag atgaattaat tgaaaatgca aatgaagaaa ttttaaaagc acataaagta
    29341 caaactgaac ttatacaaaa ggaagctggg ggagataaat cagatatatc tattttattg
    29401 attcactctc aagaccattt gatgacttgt atgagtgaga gaaaccttat aaaagaaatg
    29461 attatgttaa gaaaagaaat tcaaaaatta aaataaaaaa attaaggggg atttaaaaaa
    29521 tgaaaagaaa agtatattta ttttgtagct ttggtatgtc aacaagttta cttgcagata
    29581 aaatgcagaa ggtagcagat gaacacaatt taccaataga agtagaggca tttccgatag
    29641 cagaaattga caaaatagtt gaagaaaaaa atccagattg tatattacta ggaccacaag
    29701 ttaaatacat gctaaaggaa ttaaaaccaa agtttgaagc tcaaggaaaa cttatagata
    29761 taatcaatga agttgattat ggaactatga atggggaaaa agtattaaaa ctcgctataa
    29821 agctaataaa atccaaacaa gcgtagaatt atagtaaaaa ataataggaa atgagggtag
    29881 atagatgaat agattggaaa gaatacttat gccattggct gaaaaaatag gtaaaaataa
    29941 attattaatt gcaattagag atggtttttt aatatcatca ccattattaa taataggttc
    30001 attattttta ttagtagcaa attttcctat aaaaggatgg aatgaatttg taggtcaaat
    30061 ttttggttca gattgggctg taaaattgca acaaccaact gtagcaacat ttgaagtaat
    30121 gactctactt gcagttttag gaattggata ttcttatgca aagcaaatag atgtagaccc
    30181 aatagcatca gcagcagttg caatagttgc cttttttata ataacaccat ttgtaatacc
    30241 atatacacca gaaggaacag aatcagtcta catggtaaca ggtataccac taggttggat
    30301 gggctctaag ggaatgtttg taggtatgat aacagcaata acctctgtaa aattatttgc
    30361 tgcagttgta aaaaaaggat ggacattgaa aatgccagat ggagttcctc caaccgtatc
    30421 aaaatcattt gcagcattaa taccaagtgc aatagttatg gtagttttct ttttagttaa
    30481 gatagctttt gaagctacac catatggaag tatacatgaa tttatattta agtttttaca
    30541 aatgccatta ttaaaattag gaaatagttt aggtgctatg gcaatcgctt atatattctt
    30601 acatttattc tggttctttg gaataaatgg aagtagtgtt gttggggctg tgtataatcc
    30661 aatattaaaa attctatcag ctgaaaattt agctgctttt caagctggtt caaaaatacc
    30721 aaacataata acaggtcaat tccaagatat gtttgcgaca tttggtggag caggtagtac
    30781 actatcatta gtcatagcta tgttattaat atgtaaaagt aaacgtataa agtcaatggg
    30841 aaaactatca attttaccag gaatatttgg tataaatgag ccattgatat ttggtctacc
    30901 aataatgctt aacccaatac tattgatacc atttgcaata gttccaacaa taaatataat
    30961 aatagcttat ttctgtatgt cagcaggatt agtgcctctt acaaatggag ttcagttacc
    31021 gtggacaact ccaattatat tctctggttt tttaacaaca ggatggcaag gtgcagtctt
    31081 acaagtagtg cttcttatac taggaatatt tatgtatata ccatttataa aaatgctaga
    31141 taagcaatac ttaagagaag aaaatgcaac tgttgaagag gaagatgatg atatatcttt
    31201 tgatgattta gtattatagg aggaagatga tgaagaaaat agttatgata ttggatcaaa
    31261 tacaagctgg agctggagga aaagagaaaa gtaatatacc tcctgctgga aaatcaagcc
    31321 cattaggacc aggtataatg atggagcaat ttttaaatga atctaaggta atagctacct
    31381 tattttgtgg ggatgaattt tttgtaaata atgaagaaga agtaactagt aagatgatag
    31441 ctatggtaaa aaaattaaac cctgatgtag taatctgtgg accaagcttt aattacgaaa
    31501 acttttctaa gatgtcagca atactgtcta aaaatataaa tgataaaaca gatataccag
    31561 cttttgcagc tatgtcagaa gaaaatatag atgtgataaa cgaatataaa aatgacatat
    31621 gtatagttaa gactccaaaa aaaggtggaa ttggtcttaa tgattcatta aataacatat
    31681 gtaaattggc aaaagcaata gcaaataaag aagatataac tttgatgaaa gaagaatttt
    31741 gttattaata agagtattaa ttattagtaa gtttaaatat tatactattt ataataagca
    31801 tacattagta aaatctatat aatttaagga ggggaatttt aacatgtggg gaatgatagc
    31861 gacatggaga atggccctag aaggaataac tgaggcatca gaacatttag aaaaaggcat
    31921 ggaagcaggt gatgctgtag agcttgctgt aagaagagtt gaagactatc cattctataa
    31981 atcggttgga tatggtggac ttccaaatga aaattgtgag gtagaacttg acgctgcatt
    32041 tatggatggt aacacattag atattggagc agttgctggt ataagagatt attctaatcc
    32101 tgtcagcata gctaggaagt taagttatga gaaggtaaat aattttttag ttggaatagg
    32161 tgctgaagat tatgctcata aaaatggatt tgaaagaaaa aatatgttga ctgatagagc
    32221 gaagttacat tataaaaaga gaaaaaaaga gactttagac aaaggattaa gtccatatgc
    32281 tggtcatgat actgttggta tgatatcttt agacaaaact ggaaaaatgt gtgcagctac
    32341 atcaacaagt ggattgttta tgaagaaaag aggtagaata ggagattctg cactatctgg
    32401 ttcaggattc tatgttgata gtagtatagg aggagctaca gctactggtt tgggagaaga
    32461 tttaatgaag ggatgtatat cttatgagat agttagactt atgaaagaag ggaagcatcc
    32521 acaagaagca tgtgatattg cagtaaatga acttgataaa cagcttatca aaagaagggg
    32581 aaaagctggt gatttatcat tagttgccat gaataataaa ggtgaatggg gagtagctac
    32641 aaatatagct gattttactt tttcagtagt aactaaagaa gaatctccta ctgtatatat
    32701 ttcaaataga gtagatggaa agactataca taaggttata tcaaatgagt ggatggaagc
    32761 atatgaggct agaataaaag caccaataga agtaagtgag taaagtagtg gttacataat
    32821 taaaatagag ttttataata aggagatttt tatgaatatc ataagtaata aaatagatga
    32881 gttaaaagaa gatttattaa gtgatattat agatattgta aaaataccta gtgtcaaagg
    32941 ggagtcagaa aatggcttcc cttttggcga aaaagtaggg gaggcactta ataaagcatt
    33001 agaaatatca gaaaaattgg gatttaaggt tagaaacttg gataattata ttggttatgc
    33061 agagcatgga gatagtgatg attatgtttg tgtaattgga catgtagatg ttgtacatga
    33121 aggagatggt tggaagcatc agccttataa gggagaagaa actaatggaa gaatttatgg
    33181 acgaggagta ctagacaata aaggacctat aatgtctgcc ttatatggat tatatgctat
    33241 taaagaatta aatttaaaat tagataagag agttagaata atatttggta caaatgaaga
    33301 aagtggattt gaagatatac catattattt agaaaaagaa aaagcaccta ttatgggatt
    33361 tacaccagat tgtaaatatc ctgttgtata tggtgaaaaa ggcatggcaa agattagaat
    33421 aaaaagtaaa atcaattatg aagaagatgt atacttaggt tttatagaaa atatgtctga
    33481 aaatgtgctt gttacatata aagaactgaa tatagaaaat agtgatacta tattagatat
    33541 aaaggtaaaa tacgatttta gttataaatt gaaagatgta ttggatgaaa taaaagctag
    33601 ttttccaaat agtatcgata tagaggtaat ttctaatttt aatcctgtat attttgataa
    33661 agagagtaat cttgttaaaa agcttcagct agcttatgaa agggttacat cattagatgg
    33721 aactccagta acaactaatg gaggtacgta tgcaaaagta atgccaaata tagttccgtt
    33781 tggaccttca tttccaggac aaaaaggaat agctcataat cctgatgaat acatggatat
    33841 agaagatata atattaaatg caaagatatt tgcaaatgca atatatgaat tagcaaagga
    33901 gtagttatgt tagaaataat aggaatgtca gtagaagacg ctaaaattat agaagattgt
    33961 ggtgcagata gaatagagtt agttagtgct ttaactgaag gtggattgac accaagtttt
    34021 ggtcttatag aaagtgttgt taacagtgta aagatacctg tgaacgtaat gataagacat
    34081 catgcgaaaa gttttgtata cagtaaagaa gatataagta taatgcaaaa agatataagt
    34141 gtggttaaag agattggtgc taatggagtt gtatttggtg tattggataa aaacaatagt
    34201 atagatgaga aaaatctaaa tgttttattg aaatgttgtg ataatcttga tgttacattt
    34261 catagagcaa tagatgaatc aaatacaata gatagtgtta aaattttgaa ggattatgat
    34321 aaaattacga atatattaac atcaggaggg aaaggaagta tagttgataa catacaaatg
    34381 ataaagaata tgatgcttag ttctaatcac ataaaaattc ttttaggtgg aggtcttaat
    34441 tttaataata tagaaaagat aaaggaatta actaaggcaa gtaattttca ttttggtaca
    34501 gcgattagaa taaataacag tccttttgaa gatatagata gacaaaagct taaacaactc
    34561 gtaaatataa tttcaagata gtatatgaat ataaaatact tagtttaaat aaagagagta
    34621 agttaattat aatttgggta tatgaatgtt aataggtatt aaatatgaaa ttattgttga
    34681 gtatgcttta taacttttaa gtagttttaa atataaataa tgaaagaaaa gatagattat
    34741 ggaggtttta gagggtttat atttattaaa aaattataaa ggtatattgg catataatac
    34801 acataatttc ctaaaattac catcttatga catgaattca catattaata aacaattaat
    34861 ggagattttt gtggatagaa ataggagtaa aaatagcttt aaataaacaa aaacaagagt
    34921 tattaacagt tttatccaca ttatccacag gtgtttgtaa taatattaat ccacataagt
    34981 atcaatgtaa ctaaaaatga taaataattc tatcaattga atagaaaaaa tcataaaagt
    35041 aaataataaa aagaggatta tgagagttcg tgtagaatat attattaaag tataaatttt
    35101 cttatactgg cataattgaa gggagatgta tttatgaaca taattatatc tggaaaacaa
    35161 atcgaattaa ctgatgcaat aaaaaataaa atcgaaacta aactaagtaa gttagataat
    35221 tacattcatc cagacactga tgtaaaagtt acagtaagtg cgagaaaagc aagacaaaaa
    35281 atagaagtaa caatagctac tgtaaatggt cctataatta gagcagaaga tatgcaggaa
    35341 aatttataca cagctataga tgttgtatat gataaattaa gtaaacaatt aaaaaaatat
    35401 aaaaagagat tacaagataa acataaagac aacaagagta taagattcca agatgcagag
    35461 ataaatctag ctgatgaaca agaggattat ggggatgaaa atcaagaatt agtaattcaa
    35521 agacgtaaga aatttagtgt taagccaatg agtgaagaag aagctgtatt acagatggaa
    35581 ttaataggac atgattttta tatgttcaag aacatagatt cagatgaaat agcagtagtt
    35641 tataagcgta ataatggtgg atatggtata atagaacatg aataaagcaa tttagattta
    35701 atcatattag ctatcgataa ataaattatc gatagctttt taaattaata ttaaaagtat
    35761 agtttttatg gtaaaatgaa taatagtaca ataaagaacg aggagagatg taaaatatga
    35821 gctttatgga taatttattc aacatggcag ataaaaaaga attaaaaaag ttcaataaaa
    35881 ctgttgatat aatagattca ttagaaccta aatttgaatc tatggcagac tcggaactta
    35941 aaaatatgac aaatatattt aaagaaagat tagcaaatgg tgaaagtata gatgatatac
    36001 ttccagaagc ttttgcagta gttagagaag tttctaagag agtgttagga cttagacact
    36061 acagagttca aatgattgga ggtatagtac tacatcaagg aagaatagca gaaatgaaaa
    36121 caggtgaagg taaaactttg gttgcaactg ctcctgttta tttgaatgcg cttactggaa
    36181 aaggtgtaca tgttgtaaca gtaaatgatt accttgcaaa acgtgataga gaccaaatgg
    36241 caaaaatcta tgagttttta ggaatgagtg taggcgttat tatacacggt caaaatccaa
    36301 aagttagaaa agaacaatat gattgtgata taacttatgg tacaaataat gaatatggat
    36361 ttgactatct aaaggacaat atggtaatac acaaagagca aagagttcaa agaggattaa
    36421 attatgctat agtggatgag gttgactcta tactaataga tgaggctaga acaccactta
    36481 tcatatctgg acctggagac aaatcaactc acttatattc agatgcaaac acatttgttt
    36541 taaccttaaa accagatgat tatgaattag aagaaaaaga taaggctgta tctctaacag
    36601 catcgggtat acaaaaagca gaagtgtatt ttaatgttga taatataaca gatatatctc
    36661 atacagaatt ataccatcat ataaatcaag ctttaagagc acatgtaata atgaagaaag
    36721 atgttgatta tgttgctaaa gatggagaaa tagtaatagt agatgagttc acaggtagac
    36781 ttatgtttgg tagaagatat tcagaaggtt tacatcaagc aatagaggct aaagaaggtc
    36841 ttaaaataca aagagaatct aaaacactag ctactgtaac attccaaaat tattttagaa
    36901 tgtacaaaaa actttctggt atgactggta cagcaaaaac agaagaagaa gagtttaaag
    36961 caatctacaa aatggatgtt ttccaagtac ctactaataa gcttatgata agggaagact
    37021 taccagattg tgtatacaaa agtgaaatag gtaaatttaa tgctgtagca caagaaataa
    37081 ttgaaaggca caaagtcaat caaccaatac ttgtaggtac agtatcaata gaaaaatcag
    37141 aattattatc acaaatacta aagaaaaagg gcataaagca cgaggtatta aatgctaagc
    37201 atcatgataa agaggcagaa ataatagcac aagctggtag acttggagct gttacaatag
    37261 ctactaatat ggctggtcgt ggtacagata tagttctagg tggaaatcca gactttttaa
    37321 ctaagagaga aatgagaaga aatggattta aagaagaaat agtaaataga gtagatactc
    37381 caatagaagg tatacctgta aaaggtaatg aaattctatt tgaagctaga gaagagtatg
    37441 aaaaactatt tgaaaagttt aagcaacaaa cacaagaaga acaaaaacaa gttgtagaag
    37501 caggtggact tgctataata ggtacagaaa gacacgaatc tcgaagaata gataaccagt
    37561 tgagaggtcg tgctggacgt caaggtgacc cagggagttc aagattctat ataggtcttg
    37621 atgatgacct tatgagattg tttggtagtg atagaatatc tggtattgta gataaaatag
    37681 gtcttgaaga agatatgccg atagaacata gaatattatc taagtctatt gaaggagcac
    37741 aaaagaaagt cgaaggtaaa aactttggaa taagaaagca tgtacttcaa tatgatgatg
    37801 ttatgaataa acaaagagaa ataatatatg cagagagaaa acgtgtatta gaaggtgaag
    37861 atttacaaga acaaatacaa tcaatgactc attctataat agaagaagca gttactcttt
    37921 atacacaaga taaaggattt gatgaagaag gatttaaaga acatatgtat aatctgttct
    37981 tgccaaaagg tagcatagaa ataccagaaa tagaaaaact taatccagtt gaaataacag
    38041 aaaaagttta tgaaatagct atgaagatat atacttcaaa ggaagaacaa gttggctatg
    38101 agagaatgag agaagttgag agagtaatct tacttcaagc tgttgataac cattggatag
    38161 accatataga tgctatggac caattacgcc aaggtattgg tcttcgtgct gtaggtcagc
    38221 aagacccagt tattgcttat aaaatggaag gttttgatat gtttgatgaa atgaataaac
    38281 atatcaaaga agatactgta agatatttat ttaatataac aatagaaact ccagtggaaa
    38341 gaaaagcagt tgtagatgtt gaaaatctat cttctccatc agatggaaca cttccaacaa
    38401 gtaaaactgt taaaaaagat gaaaaggttg gaagaaatga cttatgccct tgtggaagtg
    38461 gtaaaaaata taaaaactgt tgtggaagat aatctcggag gattaaatgt taaagttaca
    38521 agagtataga catagtatgg agcagatggc ttcaaattta gaagaattga gggtttctct
    38581 ttgacatagc ttcattgatg aaaaatatac aaattaatga agaaaagatg aataaacaag
    38641 acttttggaa tgataatgaa gtcgctcaaa gagtacttca agaaaataag tctttaaaag
    38701 agacattaga agaatatgaa agcttaaaaa gcttactaga agatatagaa gtattaattg
    38761 aaataggttt agaagaagat gatgattctg tagaaagaga tatagaaaag tccatagaat
    38821 caatggaaga aaaattatct gaaatgaaaa taaagactct tctaaatgga gagtatgata
    38881 aaaataatgc tatattatct ataaatgcgg gtactggtgg cttagatgct caagattggg
    38941 ctcaaatgct tttgagaatg tatataagat ggtcagaaag taagggttat aaagttaagc
    39001 tcttagatat aatatcagac cctgaagcgg ggataaaaac agctacaatt cttgtagaag
    39061 gaacaaatgc ttatgggtat ttaaagagtg aaaaaggagt tcatagatta gtgagaatat
    39121 ctccatttga cccatcagga aaaagacata cttcatttgc atctatagat gtaacaccag
    39181 agttagatga aaatatagaa gtagaaatta atccatcaga tttaaagata gacacatata
    39241 gagcttctgg agctggaggg caacatgtaa atacaactga ttcagcagtt agaataacac
    39301 atatccctac tggtgtagta gttcagtgcc aaaatgaaag gtctcagcat ctaaataagg
    39361 acagagctat gagactttta atggcaaaat tgatagaatt aaaagagtta gaacaaaagg
    39421 aaaaaataga agatatacaa ggcaaatact cacaaataac ttggggtagt caaattaggt
    39481 catatgtttt tcaaccatat aaattggtta aagaccatag aacaaatgct gagtttggaa
    39541 atgtagatag tgtaatgaat gggaatatag acttgtttat taatgaatac ttaaaaatga
    39601 ataaaatagt ttaataaata tgcactgtaa ttaagatata tgtataagaa ggttagtgct
    39661 taatatgcac ttacagcact taggctgagg acgataaccc tcagccttcg ttctgttttg
    39721 ttatacttat gaaaaactaa cattaggagt gaataaattg gatataaatc aaattttaaa
    39781 aaaagagttt aatcttagag atgaacaaat aaataataca ttaaaactta ttgatgaagg
    39841 aaatacaata ccatttatag ctagatatag aaaagaaatg actggcgaaa tgagtgatgt
    39901 tacactaaga gaattttatg aaaagttaat gtatttgaga aatcttcaaa gtagaaaaga
    39961 tgatgtagtt agactaatag atgaacaagg taaattaaca gatgaaataa cacaaaatat
    40021 agaaaaagca aaaacactac aagaagtaga agatatatat gctccatata aacaaaagaa
    40081 aagaactaga gcaacaatag caaaagaaaa aggattagaa aaattagcat tgtcaatact
    40141 agagaataat ttagataata tagaaataga ggcaaagaat tatcttgatg aagagaaaga
    40201 agttctttct attgaagatg ctctaaaagg tgctagagat ataattgcag aactagtctc
    40261 agatgatgct aagattagaa agtatataag agaattagct ttaagagaag gtatgatagt
    40321 tagtaaaagt gccactgatg agaaatcggt ttatgacatg tattatgatt atagtgaagc
    40381 agtaaaaagt atgcctccac atagagtttt ggcaataaac agaggagaaa aagaaaactt
    40441 cctaaaggtt aagttagaaa taaataatga taaagtttta aattacataa taaacgaata
    40501 tgtaaatgat aaaaatttca aaaataaaga agaaatcgta agttcaatag aagattctta
    40561 taaaagatta attttcccat caatagaaag agaaataaga aatcatttaa cagaaatagc
    40621 tcaagaaaga gctataagtg tatttggaaa gaatgttaaa agtttattgc ttcaaccacc
    40681 agttaaagac aaagtagtta tgggatttga ccctgcattt agaactggat gtaaaatagc
    40741 tgtagtagat aaaaatggta aactactaga ttatacaaca gtatatccaa cagaccctca
    40801 aaatgatgta gagggagcaa agaaagtttt aaaaggattg attgaaaaat acgatataga
    40861 tataatttca attggaaatg gtactgcatc tagagaatca gaaacttttg tatcagaaat
    40921 gataaaagaa atagataacg aagtgcagta tgtgatagtt agtgaagcag gagcatctgt
    40981 ttattcagcc tctgaacttg caaatgagga acatccagat ataaatgtat caataagagg
    41041 agctatatct atagctagaa gattacaaga cccattagca gagcttgtaa aaatagaccc
    41101 taaaagtatt ggcgttggtc aatatcagca tgatttaaac aaaaagagat tagaagaagt
    41161 actagatgga gttgttgaag attctgtaaa tagtgttgga gtagatttaa atactgcatc
    41221 atactcccta ctagaacatg ttgcaggaat aagtaaaact atagcaaaaa atataatagc
    41281 atatagagaa gaaaatggag attttacgtc aagagcacaa ttgaaaaaag taaagagact
    41341 aggaccacaa gcatttactc aatgtgctgg atttatgaga atcttagagg gtaaaaatcc
    41401 actagataat acaggtgtgc atcctgaaag ttatgatata tgtaaaaaaa tgattgagat
    41461 aattggatat tctttagatg atgtaaagaa taagaatata tgtgaaatag atgaaaaaat
    41521 aaaagaaatt ggcttgagag aattaagtga aaaactggaa gtagggcaag ttacgttaaa
    41581 agatatcata actgaaatca aaaaaccagg aagagaccca agagaagaag gtataaaacc
    41641 tatacttaga actgatgtct taaaaattga agatatacaa gagggtatga ctttaaaagg
    41701 tacaataaga aatgttgtag attttggtgc atttgtagat attggaataa aaaatgacgg
    41761 attagttcat aaatcagaaa tgagtaatag ttttgtgaaa gaccctatga gtattgttac
    41821 agtaggtgat atagtagatg taaaagtcat tggaattgac ttaaacaaaa aaagagttgc
    41881 cttatcaatg aaaaaataga taagtttatg tatggttata gtacttaaat tctatgtttg
    41941 taaatgaggt tactcgagtg tataaatgtt aaaaatttgt aaaagtatac agtaagaaaa
    42001 tatattaaaa taagaacgat agtcttgaaa atcaggagga aaatgaatga tttttataaa
    42061 aaatggtaaa ataaatacca taacaaatgg gataattcat ggagatatac ttatagatga
    42121 aggaaaaata atagaaattg gagaagattt gatagctcct ttagatgtag aggtaataga
    42181 tgctagtaat aaacttgtgt ttcctggatt tatagatgct catactcatt taggtttgtg
    42241 ggaagatggt atagggtttg aaggagcaga tggaaatgaa gagactgacc caataacacc
    42301 acaattaaat cccatagatg gaattaatcc aatggataga acttttaaag aagcttttga
    42361 aggtggaata acttctgttt gtacaacccc tggaagtgca aatgttatgg gtggtcaatg
    42421 tatagctata aagacatgtg gaaaaagaat tgataaaatg gtaataaaaa atccagttgc
    42481 atctaaaatc gcttttggag aaaatccaaa gagctgttat ggtcaggatg ataaatctcc
    42541 tcaaactaga atggctatag ctgctttact tagagaaaac ctaaaaaaag cagaagaata
    42601 tttagaggat attgatatgt atgagagtca tgatgatgaa gactgtgaaa aacctgaata
    42661 tgatataaaa atggaaagtt taataccagt tttaagaaga gaaattcctt ttaaggcaca
    42721 tgctcataga gcagatgata tgtttacagc aataagaatt gcaaaagaat ttaatttaaa
    42781 gcttacatta gaccattgta cagaaggtca tcttattgta gatgaattag tggaagaagg
    42841 gtttcctgta atagtagggc catctttgtc agaaaggtct aaatttgagc ttagaaattt
    42901 gacatttaat acagcaggaa tactttcaaa tgcaggtctt gatgtatgta taatgacaga
    42961 ccatccagta ataccagttc aatatcttcc aatatgtgca ggtattgcag tcaaacatgg
    43021 tatgaaagaa gaaaaagcaa tagaatcaat tacgataaat ccagctaaga ctttaggaat
    43081 agaagataga gtaggctcta tagaagttgg taaagatgca gacctagtaa tttgggataa
    43141 ttctccacta gagattcaaa gtaatgtact ttacactata ataaatggca aagtagtgta
    43201 tgagaaaaaa taacttgaca attattaaaa aattaactaa tataagagtg taaggaaaat
    43261 taaaaaatca aagaagatct tcggggcagg gcgaaattcc ctaccggcgg tatagcccgc
    43321 gaactactta agtggttgat ttggtgagat tccaaagccg acagtaaagt ctggatgaga
    43381 gaagatattt atggaagata aataagttta ttttagtttg tttaaactat atccataaaa
    43441 actcgaagat agggtcttcg agttttttgt ttttcctaaa aaatataaac atattgatta
    43501 gggggaaata attatgcaaa acacagccaa aagaggcaat atgatttcaa caagaacttt
    43561 agtagtaatg tcaattttat ctgcaatatc ttatttatta atgtttattc aagcaccaat
    43621 accaggtata tttccagatt tcttaaaaat tgacttatca gatattccag caatattcgg
    43681 aggaatgtct ctaggaccat ttgttggatt tggaatagtt gtagtaaaaa atgtgttaca
    43741 agcaataaca gcaacaacaa ctggaggaat aggagaattt gcaaatatat taattggtgg
    43801 ttcttatgta atgatacttt gttattcata taagagaagt ggagatttaa aaggtgtatt
    43861 gattggtttt gtattaggaa caatatctat gactataatt ggttcattag taaattattt
    43921 tgtaatgttg ccactttatg gtcaacttat gggactagat gctataatag gtttaggttc
    43981 agctataaat cctcaagttc atgatttatt cacttttgta atttggatga tagctccatt
    44041 taatgtgctg aaggctgtta tactatcttt agtaacattg ccactttata aaaaaatggg
    44101 aaatgtaata aagaaataat tagacaagaa aatataaaat taaatgtatt gaatagccat
    44161 aatttattta taacttcagt cctatattgt gattgatata ttaaatatat tatggcttta
    44221 ttaatttatc aaaaaatgta tataaaataa gaaatattta gacaaattta aattttaaaa
    44281 taaaggaaga attaaaacga ttaactatta aaatatgaaa aaggtatctg caatatacag
    44341 agcgaattta taataaataa aaaataaagt tattattaaa aaaatataaa gattagtaat
    44401 caaattttta ataataacta atttaataat gatgggagtg tatattcatg aacttatcta
    44461 aaaagataaa aaaattattt ttagatgata aagggtatta ttttaatagt aattggaaag
    44521 atattcatat tggtatttat aatacaaaat taggagattc tttaaagtat atcactaatg
    44581 aatatattga aaatctggaa agggtaaaat atgactatcg tgaattaaaa aatatagaaa
    44641 atcaaactga tggtatgtgt attttagcaa taaggcagaa ttacaaggca ttagagtatg
    44701 taaaaaatca aaccgaagaa ttatgtttag aagcaataaa gtataattat aaagcacttg
    44761 aatatgtaaa agagcaaaca gaatatatat gtcttgaggc tataaaaaag gattgtaatg
    44821 cacttaagta tgtacgaaat aaaacagaaa ggcttattat aaaagtaata cgtcattctt
    44881 ccaatataga tgttgtaagt atactaaagg ctttagagac tcaaacaaga agaatatgtt
    44941 tagaagcaat taaaaaagat ggcaggtgtc ttgcatacgt tagagaacag agtgaagaat
    45001 tatgtataga ggccattaaa caaaattata aagcattaaa atatgtagaa aatcaaacgg
    45061 aaaaaatgtg tatagaatcc gtaagacaaa atggtatggc attacaatat gtaaataaac
    45121 aaactgataa aatatgtata gaagctgtca aacaagatgg aagaagtcta caatttgtca
    45181 ataataagac agaagaaata tgtataaatg ctataagata tttaaataaa aaatataata
    45241 ttaaagatgt actaagttac atagataagt atacagaaga tatatatata gagattgtaa
    45301 gacaaaatgg aaaaatgctt atgtatataa aaaatcaaac agaaaaaatg tgtattgaag
    45361 cagtaaaaga gaactataag tctcttaagt atgtaaaaga gcaatctgag agaatatgta
    45421 aagaagcatt gaagcaaaac cataaagcaa aagagtatgt aaaaattgct attgatgatt
    45481 gtatataagg tgtagataca gccatcaata gtgaaagata aaaaaagata aagacaagta
    45541 ctttataata atctaggctt atactataat taattaagca gtgatataaa ttcacatgac
    45601 gattatatca aaatggagat gaattgatgg caaaaatata tttagaaaat gaaaataaaa
    45661 ctagagaaat aggatataaa ttaggtaaac ttttaaaaga aggcagtgtt atctgtttag
    45721 taggagattt aggagctggt aaaactacaa tgacacaaag cttggcagat tcattaggga
    45781 tagaagatta tataacaagt cctactttta ctataataaa tgaatatgaa ggaaaaatac
    45841 ctttgtatca ttttgatgtt tatagaatag gtagtagtga cgaaatgtat gatattggtt
    45901 atgatgaata tgtaaattca aatggaatct gtataataga atgggctaat ctaatagaag
    45961 atatacttcc aaaagaatat ttgaatatag aattgagata taaagatgac ggaagagaaa
    46021 tgatattaac tccaaaagga gagttttata aagagatagt tgaggagttg attaaataat
    46081 gaaaatacta ggtatggata cgtcttctat ggcagcaagt gtagcagttg ttgaagatga
    46141 taacttaata tgtgagttta cagtaaacaa caaaaaaact cattcacaaa agcttatgcc
    46201 aatgatagaa aatatgttga gtatgagtga tttaagtata aaagatatgg atttattagc
    46261 tgtatgtata ggtccaggct cgtttactgg acttagaata ggtatggcca cagtaaaagc
    46321 tatggctcat gtaaataata taccaataat agcagtaaat tctttggaat ctctagcaaa
    46381 taacataaat ttctgtgata gaaagatatg ttgtatatta gatgcgcaga aaaatcaagt
    46441 ttattcagct aaatacaagt ttgaaaatgg agagatgata gaattagatg gtgtagatgt
    46501 tgtagagttt gatgctttag tagatgaaat tgtatcaact aatgaagaat ttataatagt
    46561 tggagaagct gtatacaaat ataaagataa gttagagaat ataaaaaata taaaaatacc
    46621 ttcaccagct aataatgtat ctaacgcagg aagcttatgc tctttagcat taaataaata
    46681 taataaaaat atagatgtac atacttgtta tacgataaat cctatgtata tcagaaaatc
    46741 tcaagctgaa gtacaatatg atgaaaaaat gaagaggcta aataatggaa gataatacaa
    46801 aactaatagt agatgatata aaaatagaag aaatgactac agaagatata gatgaagttt
    46861 ttgaagtaga aaaaaattgt tttgaagatt attggtcaaa agagtctttt agaaaagagt
    46921 tgtctaatga agtagcaagg tatatagtag ctaagttaaa tggaaaaatt gtcggatatg
    46981 tgggaatttg gcttatactt gatgaagggc atataaataa tgtggctgtt catagtgact
    47041 atagagggaa aaaaataggg gacaagttga taaaaggcat agttgattta tgtaaagata
    47101 ataatattgc atctatgaca ttagaagtta gagcatcaaa taaaatagca caaaatttat
    47161 atagaaaata tggatttaaa atgggtggaa taagaaaaga atactacagt gataataaag
    47221 aagatgcaat tataatgtgg aaccaattaa aagaggtgta gagtttatga gtgatataat
    47281 aacattagct atagagagta gttgtgacga aacagctgca tcagttttaa aaaatggtag
    47341 agaagtactt tcaaacataa tctctactca aatagagaca cataaaaaat ttggtggtgt
    47401 agttccagaa gtggcttcaa gaaaacatgt ggaaaatatt gatatagttg ttcaagaagc
    47461 tttagacaaa gcaaatatag gttttaatga tatagaccat atcgcagtta catatggacc
    47521 tggactagta ggtgctttat tggttggtct atcttatgct aaagcacttg catatacttt
    47581 gaatattcca cttgtaggag taaatcatat agaagggcat ctaagtgcaa actatataga
    47641 gcataaggat ttgaaacctc cttttataac attaatagta tctggaggtc atactcactt
    47701 ggttgaagta aaagattatg gcaagtatga aattttagga aaaacaagag atgatgcatc
    47761 tggagaagca ttcgataaaa tttcaagagc aatgaattta ggttatccag gaggacctat
    47821 tattgataat ctagccaaga agggaaataa acatgctata gagtttccaa gagcctattt
    47881 agaagaagat agttatgatt ttagttttag tggattaaaa tcttctgtat tgaattatct
    47941 aaatgggaaa agaatgaaga atgaagaaat agtagttgaa gatgtagcag catcttttca
    48001 agaggctgtg gtagaggttt tatctactaa ggcactaaaa gcagtaaaag aaaaaggata
    48061 caatatcata actctttctg gtggagttgc ttctaattcg gggcttagag ctaaaataac
    48121 agagcttgct aaagacagtg gtattactgt taagtatcct cctctaattt tatgcacaga
    48181 taatgcagct atgatagggt gtgcaggata ttataatttt ataaacggga aaacgcatga
    48241 tatgagttta aatgcagttc caaatttaaa gataaatcag tagagttaga taaatttgta
    48301 aatataaaag tattgtttca aaaaagtaag cagaagtgaa attaaatata ataaaaatca
    48361 atatagtata tattaaaact tgaagtaaaa ctatttataa tctttatatt ccaatgaatt
    48421 gcaacacatt ttaggatata caggttgtct ttggcttaga tttgcttcaa gtttaatttt
    48481 ttctatagaa agattatttt aaaaagtggg taatataccc ttttttttca ttcttaaaaa
    48541 gttttatact aattatagaa agattaagga ggggaagaaa tgagtcaaag taaagaagac
    48601 aaaataagaa gtattttgga agcaaaaaat atcaaatcaa attttcaaaa taaagaaaat
    48661 ttaagtgaat ttaatgaaaa aaaagcaagt aaaagagcag aagacttatt agatgtatat
    48721 tataatacat tatctactgc agatatggaa tttccatatt ggtataatag agaatataga
    48781 aaatcagatg gtgatatacc agtagttaga agagctaaag ctcttaaatc tgctttttca
    48841 catatgacac caaatataat acctggtgag aaaatagtaa tgcaaaagac acgccattat
    48901 agaggttctt ttccaatgcc atgggtaagt gaaagtttct ttgttgctca aggagaacaa
    48961 atgagagaag aggcaaagaa attagccagt aacacagctg atgaacttac aaaatttggg
    49021 tctggcggag gaaatgttac tgaaagtttt ggaaatgtcg tttcaatagc tggtaaattc
    49081 ggaatgagaa aagaagaagt acctgtactt gtaaaaatgg caaaagaatg ggttggaaag
    49141 tctgttgaag atttaggttt tcactatgaa aaaatgatgc cagattatga tttaaaagaa
    49201 aacctaatga gtacacttat atgtatgttt gattcaggat atacacttcc tcaaggtaga
    49261 gaagttataa attattttta tcctttaaat tatggattag atggaattat agaaatggca
    49321 aaagaatgta aaaaggcagt tgcaggaaat gcatctggag atgggcttat tggtatggac
    49381 cgtctatatt tttatgaggc tgttattcaa gttattgaag gattacaaac ttggatttta
    49441 aattatgcaa aacatgcaaa atatttagaa agtatagaga cagatttaga agctaaaaaa
    49501 gaatactcag atttagtgga gatattagaa catattgcac ataaacaacc tcgtacattt
    49561 agggaagctc ttcagttgac ttatacaatt catatagcat cagtaaatga ggatgctata
    49621 tctggtatgt ctataggccg ttttggtcaa attttatatc cttggtatga acaagatata
    49681 gaaaaaggat taattacaaa agaagaggtt atcgaattat tagagcttta tagaataaaa
    49741 atcacttgta ttgactgttt tgcttcagcg ggcgtaaatg gaggagtact atcaggaaat
    49801 acatttaata ctttatctat aggtggatta aaagaagatg gttctacagg agctaatgaa
    49861 ctagaggagt tattattaga agcaagtatg agatgtagaa ctcctcaacc tagtttaact
    49921 atgttatatg atgaaaaact tccagaagat ttcttaatga aagctgctga atgtacaaag
    49981 cttggttctg gttatccagc ctgggtaaac aactcaaatg gaacaacatt tatgatgaaa
    50041 caatttgctg atgaaggtat gactgttgaa gaagcgagag catttgcatt aggaggatgt
    50101 ttggaaacgt ctccagggtg ctggaaacag ttaactttaa acgggaagac atactctatt
    50161 gcaggtggag caggtcaatc tgctggctct ggagttcact ttatagctaa tcccaaaatt
    50221 ttagaacttg ttcttatgaa tggtaaagac catcgtatga atatacaagt atttgagcct
    50281 cataataagc cattagatac ttatgaagaa gtcatagaag tatttaaaga ttattataaa
    50341 caagcaataa atgttttaga aagagcaaat aacattgagt tagatatatg gagaaaattt
    50401 gatacttcta taataaattc actattaaag ccagactgtt tagacaaagg tcaacatata
    50461 ggaaatatgg gatatcgtta taatgcaaca ttaaacgtag aaacttgtgg tactgttact
    50521 atggtcaatt catttgcagc acttaagaaa cttgtatatg atgacaaagc atttacaata
    50581 gaggaaatga aggatgccat attaaataat tttggattta aagatgcatt agaagtagga
    50641 aattattcta tggcagacca agtaaaagta gataagactg gtaaatatga tgctatttat
    50701 aaggcctgtc ttgatgcacc taaatatgga aataatgatt tgtatgcaga taatattctt
    50761 aaaaactatg aggtatggtt atcaaaagta tgtgaagaag cacaatcttt atatgctaag
    50821 aagatgtacc cttgccaaat atctgtttct acacatggac cacagggagc tgctaccttg
    50881 gctactgcag atggtagact aagtggaaca acatattcag atggttcagt ctctgcatat
    50941 gcaggaacag ataaaaatgg ggtatatgca ttatttgaat ctgcaactat ttgggaccaa
    51001 gcagtagttc aaaattctca gatgaattta aaacttcatc caacaactat aaaagggcaa
    51061 caaggaacta aaaaattatt agatttaaca agaagttatt taagaaaagg tgggttccat
    51121 attcaatata atgttgtaga ttcagaaaca ttaaaagatg cacaaaaaaa tcctgacaat
    51181 tatcgtcaat taatggtacg tgttgctggg tttactcaat attggtgtga attaggtaaa
    51241 ccaatacaag atgaagtaat tgctagaaca gagtatgaag gggtgtaaaa tatgagaaag
    51301 catagtgatt gtatgaattt ttgtgcagta gatgcaacca aaggaatttg tagattatca
    51361 aaacaaatga ttaatttaga tgatgcagca tgtccagaga taaaagtaat gccaaaatgt
    51421 aaaaattgta aaaattttgt tgaagctaat gatgaaggta taggcaaatg tgttggccta
    51481 gagaaagaag actgggtata ttcaacattg aatgcaatta cttgtgaagg acatgtgttt
    51541 aatgagtagt caaaagcaat tagaaggcat gattttcgat gtacaaagct tttcagttca
    51601 tgatggtcca ggttgtagaa caactgtatt tttaaatgga tgtccactga gttgtaaatg
    51661 gtgtgcaaat ccagaaagtt ggactgtaag accacatatg atgtttagtg aattatcttg
    51721 tcaatatgaa aatggatgta ctgtatgtca tggtaagtgt aaaaatggtg cattgagctt
    51781 taatcttgat aataagccag ttatagattg gaacatctgt aaggactgtg aaagttttga
    51841 atgcgtcaac tcatgttatt ataatgcgtt taaattatgt gcaaaacctt acactgtaga
    51901 tgaacttgtg caggtaatta aacgtgattc taacaattgg agaagtaatg gaggagtaac
    51961 atttagtgga ggtgagccac tacttcagca tgaattttta catgaagtat tattaaagtg
    52021 tcatgaggta aatatacaca ctgccattga aacgagtgca tgtgtatcaa atgaagtttt
    52081 taataaaata tttaacgata ttgattttgc atttattgat ataaagcata tggatagaga
    52141 aaaacacaaa gaacaaacag gtgtgtataa tgatttaata ttagaaaata tatcaaacct
    52201 tgcaaattct gattggaatg gaagattagt acttagagta ccagttatct ctgggtttaa
    52261 tgatagtgat gaaaatataa gtgatattat atcttttatg cacaaaaata atttggtaga
    52321 aattaatcta ttaccatttc acagattagg agaatcaaaa tggattcaat taggcaagga
    52381 atatgagtat tctgataagg gtgatgttga tgaaggtcat cttggagaat tacaagatat
    52441 atttttggat aatggtatag cttgttatgt tggtcatgag acagctttct aaaattaaat
    52501 acaagtttta attaaaaaag gatgttacac taaaagctag agttacatcc ttttttatgt
    52561 ggtttttatg tagaaataat taagtaaagt tttatatatt ttatatattg tttatattgc
    52621 tttatgtcca taaatattgc tttaaatata ctttatatat tttttatagt ttaaattaca
    52681 gttagagaat atattacgtt tactttattg aagcagaagt tttgtggtaa aatactacta
    52741 tgaaaaaatt taatagaata tattttagaa acaactaaaa ataatagtta ggaggaagac
    52801 atctatatga acaaatcatg gagcaacttg attattaaga ataaagacaa aaaagtacgt
    52861 tcagggtgga ttattatagc tgttatggct accttttaca tatttcagta ttatttaagc
    52921 atgatacttg tggaaataat aagaaaaatc ctggttgcta caggggacat taatttaaaa
    52981 acaaactatt tttcagaata tgctaattgg ttaaatgatg ttgggttaca gattgtattt
    53041 caaattctaa cagattcact tatgattatt attcctataa ttgtatggag atttattatg
    53101 aaaaatccta ttagtgaaat gggattacat tcttatagaa ttaagaaaaa agaaggatat
    53161 gtaggaatgt tattaggaat atttaattgt acagtaatct ttattataac aataacaatt
    53221 ggtggtggtc atgttacttc atgggtacca aaaatttcac ctttaacagt ttcttgaata
    53281 cttgtcttta ttttagttgc ttttgcagaa gaaattttaa atcgtggttt ttttatggca
    53341 gttttacgaa gatgtaaaaa tatttatttt ataatgattg ttccatctgt tatttttggg
    53401 ttgattcata tatggaatcc tgatgttact tttttgtctg taattaatat tataattatt
    53461 gggattcttt tttcatatat gtttataaag tcgtcgaata tatggatgtg tataggttat
    53521 cattttacat ggaatgtatt tcaggggatt atatatggta tgcctgtaag tggacttcag
    53581 gttccaggga ttataactac acaaatcact catggtaatc tattaaatgg tggtatgttt
    53641 ggtattgaag gtggaatttt gactactttt gttaatctac ttagttttat atttgtatgg
    53701 tattactata gagattctaa gtatgatttt gttagtgata aagtaaatgt gaccaatatc
    53761 aagaattagt tgattataat aaattttaat aatatcttct aagcttaaag tattctgtag
    53821 gattgttgga aatttacaaa aatataaaaa taacttggat attctattta ctccaagtta
    53881 tttttatatt cttttgtaaa tagaaatgga acataaaaaa ttaaaacaac catagatact
    53941 attagtatat catggtaagt gatagttgat tctttagaat ttgtgataag aaaagcacta
    54001 ttatttaaaa tatgaattat agcacaaaac cagatgttct tagtttttat ataaagatat
    54061 cctaaaaata ttgaaattcc aactatatgt atcgaacgta atattaatcc taatattggt
    54121 gtttcaggtg tatataatgt aaatattagt ggtaagtgcc acaaactcca acaaatacct
    54181 aatattataa ctcctagttt tttaccaaat ttattaaaaa aaatattctg taaaaaacct
    54241 ctccaggcat attcttcact aaaaaaaata taaatagtag ttaatgatgt tattggagta
    54301 aatataaatt ttatgatacc actataatta ggttgagacc cttgtagtat taatataaat
    54361 gatgttggaa caattacaga aatcataaag taaataatta acaaagaacc tagtttaata
    54421 ttttttatag gatataaatt ttttttattt gatgaatata ttat
//
LOCUS       DAMJWU010000011        54068 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMJWU010000011 DAMJWU010000000
VERSION     DAMJWU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.011"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..679
                     /locus_tag="PC460_000853"
     CDS             <1..679
                     /locus_tag="PC460_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599751.1"
                     /translation="LSISKNIMPLPENVNLNDISTKLVEVNAGFKVGKTSIINTLALK
                     NIEASLNNTLVELSEKIKTSFDSSDTSVQDLQNQVTQKNNTITQLETELSKRKRFITG
                     TYTFTKTDAENFNLSIYDKEGTSKTLTIPVNMGFSPSLIVLSGVTFSTTSKSYVYFDN
                     VCNSNFYNFGYNSDSTHSNPKAVGILNVSNVGYSSLVLTLYKLSMSEAVGIWAKEGAT
                     LTYKIYI"
     gene            700..1047
                     /locus_tag="PC460_000854"
     CDS             700..1047
                     /locus_tag="PC460_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599752.1"
                     /translation="MNRNNRIIYDQTGNIWLQTGEATGDIQEWSKITELNFLDVEFGS
                     IDYSKQYIESINPVTKEPIIKDIEVILTDEQKRLQALEKELSMLKEENKNRDSEIVNT
                     AFEVENIKLNNNL"
     gene            1059..1229
                     /locus_tag="PC460_000855"
     CDS             1059..1229
                     /locus_tag="PC460_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599753.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKIDVFYAVNRITEEQYLELTSL
                     LNKEETPAEPIK"
     gene            1260..1826
                     /locus_tag="PC460_000856"
     CDS             1260..1826
                     /locus_tag="PC460_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599754.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            1857..2147
                     /locus_tag="PC460_000857"
     CDS             1857..2147
                     /locus_tag="PC460_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599755.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            2151..2408
                     /locus_tag="PC460_000858"
     CDS             2151..2408
                     /locus_tag="PC460_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2599756.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGVNQTYKQFKDGEK"
     gene            2669..3532
                     /locus_tag="PC460_000859"
                     /pseudo
     CDS             2669..3532
                     /locus_tag="PC460_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
     gene            3611..4423
                     /locus_tag="PC460_000860"
     CDS             3611..4423
                     /locus_tag="PC460_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2599757.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            4517..6916
                     /locus_tag="PC460_000861"
     CDS             4517..6916
                     /locus_tag="PC460_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2599758.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NINLKNNNLINELHCDANKLSSLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLNLSNNVFLKNLSCSGNNLSKLNIKGLTSLE
                     YLGCAKNKLQDLDVSTNIKLDSLFCGKNSLTKIDISKNKELKSLECSGQYIEKSIIKN
                     GENIVVDIGIENSKLKELYPQNYNISTGFLTWKNISEFPNNVSYSLEYEYGPVNDVET
                     MDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFNGWRNNKD
                     EKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKLTGADR
                     NETSVKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKE
                     INRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNL
                     SKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGG
                     ESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLV
                     DALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLV
                     K"
     gene            complement(6957..7142)
                     /locus_tag="PC460_000862"
     CDS             complement(6957..7142)
                     /locus_tag="PC460_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2599759.1"
                     /translation="MILLEQRKLRVAFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(7690..7866)
                     /locus_tag="PC460_000863"
     CDS             complement(7690..7866)
                     /locus_tag="PC460_000863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599760.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            8583..9260
                     /locus_tag="PC460_000864"
     CDS             8583..9260
                     /locus_tag="PC460_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2599761.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            9434..11470
                     /locus_tag="PC460_000865"
     CDS             9434..11470
                     /locus_tag="PC460_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF2599762.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(11661..12227)
                     /locus_tag="PC460_000866"
     CDS             complement(11661..12227)
                     /locus_tag="PC460_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2599763.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            12515..13450
                     /locus_tag="PC460_000867"
     CDS             12515..13450
                     /locus_tag="PC460_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2599764.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            13915..15129
                     /locus_tag="PC460_000868"
     CDS             13915..15129
                     /locus_tag="PC460_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599765.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETMNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(15549..16070)
                     /locus_tag="PC460_000869"
     CDS             complement(15549..16070)
                     /locus_tag="PC460_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2599766.1"
                     /translation="MKFKRLRTSENKLCYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            16426..17223
                     /locus_tag="PC460_000870"
     CDS             16426..17223
                     /locus_tag="PC460_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2599767.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGIKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            17333..17923
                     /locus_tag="PC460_000871"
     CDS             17333..17923
                     /locus_tag="PC460_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF2599768.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            17989..19686
                     /locus_tag="PC460_000872"
     CDS             17989..19686
                     /locus_tag="PC460_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2599769.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            19900..20739
                     /locus_tag="PC460_000873"
     CDS             19900..20739
                     /locus_tag="PC460_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF2599770.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            21256..23112
                     /locus_tag="PC460_000874"
     CDS             21256..23112
                     /locus_tag="PC460_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2599771.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    23195..23435
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    23501..23736
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            23821..25038
                     /locus_tag="PC460_000875"
     CDS             23821..25038
                     /locus_tag="PC460_000875"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2599772.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            25155..25649
                     /gene="cdeM"
                     /locus_tag="PC460_000876"
     CDS             25155..25649
                     /gene="cdeM"
                     /locus_tag="PC460_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF2599773.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGENRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            25914..27179
                     /gene="hisD"
                     /locus_tag="PC460_000877"
     CDS             25914..27179
                     /gene="hisD"
                     /locus_tag="PC460_000877"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF2599774.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(27382..28134)
                     /locus_tag="PC460_000878"
     CDS             complement(27382..28134)
                     /locus_tag="PC460_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2599775.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(28252..28521)
                     /locus_tag="PC460_000879"
     CDS             complement(28252..28521)
                     /locus_tag="PC460_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599776.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            28996..30090
                     /locus_tag="PC460_000880"
     CDS             28996..30090
                     /locus_tag="PC460_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2599777.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISNITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            30092..31042
                     /locus_tag="PC460_000881"
     CDS             30092..31042
                     /locus_tag="PC460_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF2599778.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKVDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            31035..32219
                     /locus_tag="PC460_000882"
     CDS             31035..32219
                     /locus_tag="PC460_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599779.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            32232..33728
                     /locus_tag="PC460_000883"
     CDS             32232..33728
                     /locus_tag="PC460_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2599780.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEECMKICEDFDFNIDPHMVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVND
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            33751..34701
                     /locus_tag="PC460_000884"
     CDS             33751..34701
                     /locus_tag="PC460_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2599781.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            34722..35684
                     /locus_tag="PC460_000885"
     CDS             34722..35684
                     /locus_tag="PC460_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2599782.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            36056..36688
                     /locus_tag="PC460_000886"
     CDS             36056..36688
                     /locus_tag="PC460_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2599783.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            37099..37191
                     /gene="kdpF"
                     /locus_tag="PC460_000887"
     CDS             37099..37191
                     /gene="kdpF"
                     /locus_tag="PC460_000887"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF2599784.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            37232..38914
                     /gene="kdpA"
                     /locus_tag="PC460_000888"
     CDS             37232..38914
                     /gene="kdpA"
                     /locus_tag="PC460_000888"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF2599785.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            38939..40996
                     /gene="kdpB"
                     /locus_tag="PC460_000889"
     CDS             38939..40996
                     /gene="kdpB"
                     /locus_tag="PC460_000889"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF2599786.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            41015..41641
                     /locus_tag="PC460_000890"
     CDS             41015..41641
                     /locus_tag="PC460_000890"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF2599787.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVEDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            41878..42786
                     /gene="cysK"
                     /locus_tag="PC460_000891"
     CDS             41878..42786
                     /gene="cysK"
                     /locus_tag="PC460_000891"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF2599788.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            42813..43403
                     /gene="cysE"
                     /locus_tag="PC460_000892"
     CDS             42813..43403
                     /gene="cysE"
                     /locus_tag="PC460_000892"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF2599789.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            43563..43751
                     /locus_tag="PC460_000893"
     CDS             43563..43751
                     /locus_tag="PC460_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2599790.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(43805..44674)
                     /locus_tag="PC460_000894"
     CDS             complement(43805..44674)
                     /locus_tag="PC460_000894"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF2599791.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDVINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(44871..46115)
                     /locus_tag="PC460_000895"
     CDS             complement(44871..46115)
                     /locus_tag="PC460_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599792.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYNMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            46406..46717
                     /locus_tag="PC460_000896"
     CDS             46406..46717
                     /locus_tag="PC460_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2599793.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      46810..46902
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            47002..47796
                     /gene="thiD"
                     /locus_tag="PC460_000897"
     CDS             47002..47796
                     /gene="thiD"
                     /locus_tag="PC460_000897"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF2599794.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            47820..48608
                     /gene="thiM"
                     /locus_tag="PC460_000898"
     CDS             47820..48608
                     /gene="thiM"
                     /locus_tag="PC460_000898"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF2599795.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            48631..49263
                     /gene="thiE"
                     /locus_tag="PC460_000899"
     CDS             48631..49263
                     /gene="thiE"
                     /locus_tag="PC460_000899"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2599796.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKQYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            49402..49704
                     /locus_tag="PC460_000900"
     CDS             49402..49704
                     /locus_tag="PC460_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2599797.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(49770..50519)
                     /locus_tag="PC460_000901"
     CDS             complement(49770..50519)
                     /locus_tag="PC460_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2599798.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(50513..51475)
                     /locus_tag="PC460_000902"
     CDS             complement(50513..51475)
                     /locus_tag="PC460_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599799.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(51479..51937)
                     /locus_tag="PC460_000903"
     CDS             complement(51479..51937)
                     /locus_tag="PC460_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2599800.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            52293..52649
                     /locus_tag="PC460_000904"
     CDS             52293..52649
                     /locus_tag="PC460_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2599801.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            52721..53437
                     /locus_tag="PC460_000905"
     CDS             52721..53437
                     /locus_tag="PC460_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599802.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            53430..>54068
                     /locus_tag="PC460_000906"
     CDS             53430..>54068
                     /locus_tag="PC460_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2599803.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYVFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITL"
BASE COUNT        21198 a         5780 c         9216 g        17874 t
ORIGIN      
        1 gttaagtata agtaagaata taatgccact gcccgaaaat gtaaatttaa atgatatatc
       61 aacaaaatta gttgaggtta atgcaggatt taaagttggg aaaacaagta ttataaacac
      121 tttagcactt aaaaatatag aagcaagttt aaataataca cttgtggagt tatcagagaa
      181 aataaaaaca agttttgata gttcagacac tagtgtgcaa gatttacaaa atcaagtaac
      241 acaaaaaaac aatactataa ctcaattaga aacagagtta agtaaaagaa aaagatttat
      301 aacaggaact tatacattca caaaaacgga tgcagagaat tttaacttaa gtatatatga
      361 caaagaagga acttcaaaaa ctcttactat accagttaac atgggcttct ctcctagttt
      421 aattgtactt agtggagtaa cttttagtac aacaagtaaa tcttatgttt attttgataa
      481 tgtttgtaat tcaaattttt ataattttgg ctataattct gattctactc atagtaaccc
      541 gaaagctgtt ggtattctta atgtatctaa tgttgggtat tcttcattag tgcttacatt
      601 atataaatta tcaatgtcag aagcagttgg aatatgggca aaagagggag ctacattaac
      661 ttacaaaata tatatttaat aaaaaaatga ggtgataata tgaacagaaa taatagaata
      721 atttacgacc aaacaggcaa tatatggctt caaactggtg aagcaacagg agatatacaa
      781 gagtggtcaa aaataactga attaaatttt ttggatgttg aatttggtag tatagactat
      841 agtaaacaat atatagagtc tataaatcca gttacaaaag aaccaatcat caaagacata
      901 gaagtcattt taacagatga acaaaagaga ttacaagcat tagaaaaaga attaagcatg
      961 ttaaaagaag aaaataaaaa tagagatagt gagatagtaa acacagcctt tgaggtagaa
     1021 aatataaaat taaataacaa tttataggag gaattaacat gtataactta ttaaaactta
     1081 tgatagaaca aaagaactat agcactaaag aggatttgca acacaaaatt gatgtattct
     1141 atgcagtaaa tagaattaca gaagaacaat atttagaact tacaagttta ttaaataaag
     1201 aagaaacacc agcagaacca ataaaatagg ttcttttttt attgaaagaa ggtgactaaa
     1261 tgacttttaa agagttagtt aataaagtta gaaatcttgt attagaagca aagaatgtaa
     1321 ctatagaaga tacagaaagt aaatttacaa gtgaaaatgt agaaggagca ttgaaagaat
     1381 gtatagatag agcagatgag gcttttcaag aagccgatag tggaaaaaca cttttatcaa
     1441 ctgctatcgg ctctcctgct acatcagaac aaacatttca agattatgcg aactatatta
     1501 caggatttaa gagcaatata agcaatttag aaactcaatt gaaaagcaaa tattctatta
     1561 gatatggtcc tattgatgga tatgatggta atcctttttc tgctaatttt ggcaagagtg
     1621 caagttacct tattgtctat gtttacttta gaagaagtgt atattattat aatcccagtg
     1681 gtagttcttt aggaagtaat acaggaggtt ctgaacgtgc atggattact atagacagca
     1741 ataaaactgg tttttcagtt cattcatatg atactagtta tgagccatat ccttttacag
     1801 gttattatat tgcttgtttc gcataataaa ttatattatt aaaactagga ggatgtatgg
     1861 aagaaattag cataaatcta ttatgtgcag ttgcaggagt tgtaatatcc tacttagcat
     1921 ttagaaatag ctcaaacaga aagatacaag atgatacaga aacaactaca aaattagaac
     1981 aacaaataac ttttctgtgt gagaatgtaa gagatataaa gcatgatgta gcaaagttta
     2041 atacaagttt cttagatatc agtgaacgag ttgcaaaagt agaagcaagt acaaaacaag
     2101 cacatcttag aattgatgaa attataaata gaattggagg aaaataaaag atggataatt
     2161 taataagttt tataccagag cagttactaa ttttagtagc tgctctttat gttataggag
     2221 caggttgcaa aaaatataaa caattagata ataaatacat tccagtagtg ttattgatac
     2281 ttggaatagg tttctcaata tggatgctag gattaaatcc tgttgcagtc ttacaaggtg
     2341 taatttgttg gggagttgca ataggtgtaa atcaaactta caaacagttt aaggatggtg
     2401 aaaagtaatg aaattaacaa aaatattatt actcacaaaa atttaaaaaa tttcattaaa
     2461 tagagtatac ttttatagct aataagtata taataatagt acagaactcg taaagcattt
     2521 atatgctcaa atatacggga cattgatttt tgaaggaacc cgccaagctt cttttatagc
     2581 tcaaatagtc gggacagata aatttaccca ctaatattaa ttggtgggtt ttattatata
     2641 aaagtatatc tatgaaaagg tgaaaacaat ggttgaagta aaagaagaaa aaacatttga
     2701 tgaacaaata gatattttaa aaagtagagg attaataata aatgataaag aagatgctaa
     2761 atttgtatta agtaatgtca attattatag gtttacagca tatcttctaa gttttaagaa
     2821 tgatgatggc tcatataaag aaggaactac gtttgaagaa gtttatgata tatataggtt
     2881 taataaggaa tttaggatat tattaacaga tttgttaggg agcatagaaa tagcatttag
     2941 gacatacatt gcatatacat tagcaattaa acatggtgct tgtggatatc tataaaggga
     3001 gagtttcaaa gatgaaaaat tctatattaa ttttttgaca gcattagaga gagaaaaaaa
     3061 taataattca gataagcttt ttattataca ccataaagaa aaatataaag gaaaacttcc
     3121 catttgggtt gcaacggaaa taatgacttt tggtatgttg taaaaattat attcaaacat
     3181 gttgccagaa gatactagat acataaaaaa taatttgtgt agagtgaata ctttattagt
     3241 taaatcttgg ttacaatcat taacacaggt cagaaatcaa tgcgctcatt atggtagaat
     3301 atataataat aatttccgta ttataacaat aaaaaacgaa tataaaaagt ataacttgga
     3361 taataaaaag atattttctt atatacttgc tatgaagcat ttgactatgg ataaattaat
     3421 ttggaatagt ttttttataa aacttcaaaa gttaattaat gattataata attctataga
     3481 cttaaagctt attggttttc ctaataattg gatagagata ttggctaaat aaaatagtta
     3541 ctttaagaag tttataaaca cttactatat gtaagtgttt ttttattgaa aagaaggagg
     3601 aaaataaata atgaaaatat gtattacagt aggacacagt attttaaaaa gtggtgcatg
     3661 cacttctgct gatggagtag ttaacgaata ccaatacaat aaatctcttg caccagtatt
     3721 agcagataca tttagaaaag aaggtcataa ggcagatgta ataatatgcc ctgaaaagca
     3781 gtttaaaact aaagcagaag aaaagactta taaaatacct agagttaata gtggaggata
     3841 tgatttactt atagaactac atctaaatgc aagtgatggt caaggaaaag gttcagaagt
     3901 tctatattat agtaataaag gtttagaata tgcaactaga atatgtaata agctaggtac
     3961 agtatttaga aatagaagag ctaaattaga taaaggatta tatatcttaa atagttcaaa
     4021 tcctacagca gtattaattg aaagtttctt ctgtgataat aaagaagatt atgagaaagc
     4081 taagaaacta ggtcatgaag gtattgctaa gttaattgta gaaggtgtat taaataaaaa
     4141 tataaataat gagggagtta aacagatgta caaacataca attgtttatg atggagaagt
     4201 tgacaaaatc tctgcaactg tagttggttg gggttataat gatgggaaaa tactgatatg
     4261 tgatataaaa gattacgtgc caggtcagac gcaaaatctt tatgtcattg gtggtgcagc
     4321 atgtgagaag attggttcta tgactaaaga aaaatttact atgataaagg gtaatgatag
     4381 atttgataca ctttataagg cattagattt tattaataga tagattatat aaaatatttt
     4441 agtatttctt gaaaatcata agtatggtaa tatttagata aatattattt attattaata
     4501 ggtatggggg tagattatga tatttaagaa gtatataaaa tcattactga tagttgttag
     4561 tgttacattg ataatgaaca aatcaatatt atttgtaaat gcattagatt tagagagtaa
     4621 caataacata aaatcttata aaagaaagaa tgtaaaggaa attgatataa gcaataaatt
     4681 tgttgatgaa tcctttagaa gagatattgt tgaaaaggta ttagataata gggatgatcc
     4741 attaacaaat gaagttgcat caaaagataa ctataaaata tatgaaagtg atttaaataa
     4801 aattgagagt tgcttagagt tagatttaca taaaaatcca gatgtttcca acccaaacaa
     4861 aatagataat ttagatggga tagaaaattt tagaaatcta gaaaagcttg attgtagttc
     4921 aaataatcta gaaagtgtag atttaagtaa aaatataaaa ttaaaatatc tagatattag
     4981 tggaaataaa atattaaaca ttaatttaaa aaacaataat cttattaatg aattacattg
     5041 tgatgcaaat aaattatcaa gtttagacct taaaaataat aaaaatttgg taagtttatt
     5101 ttttactagt aatgaattaa cagagataga tttaagtaat aatgtgaact tagaaaattt
     5161 gcaatttctt aataataatt taaaaagtat agatttaagt cataataaaa agttgaaatc
     5221 tctaaattgt actaacaata aacttaaatc tcttaattta agtaataatg tttttttgaa
     5281 aaatttaagt tgttcaggta ataacctaag taaattaaat ataaaaggac ttacaagtct
     5341 tgagtatttg ggatgtgcta agaataaatt acaagattta gatgtatcta caaatataaa
     5401 attagattct ttattttgtg ggaaaaatag tttaacaaaa atagatataa gtaaaaataa
     5461 agaattaaaa tcattggaat gtagtggtca atatatagaa aaaagtatta taaaaaatgg
     5521 tgaaaatata gttgtagata taggaattga aaattcgaaa ttaaaggaat tatatccaca
     5581 aaattataat atctcaacag gttttctaac atggaaaaat atttctgaat ttccaaataa
     5641 cgtaagttat agtttggaat atgaatatgg tccagtaaat gatgtagaga ctatggatgt
     5701 aaatatatca atagataaat tagttaaatg tagaatcaga tatatagatt atgatggaag
     5761 tataatcaat gaaaaagaaa tatcagataa ttctagtata aatgaaattc aaattaatcc
     5821 tcaaagagaa aactatgagt ttaatggttg gagaaacaat aaagatgaaa aaatatatac
     5881 atcagagcag gtaagtaaat tagtttttga tgatagtact gattttatag caacttataa
     5941 aagaaaatca acaggatcaa gtggttcttc tggtggaact tcaagtaaag aagattttaa
     6001 tatagcacca tctaaggaaa aattaacagg tgcagacaga aatgaaacat cagtgaaaat
     6061 aagtcaaaaa ggatggaata aagctgataa tatagtgtta ataaatgact ctagcatatc
     6121 ggatgcttta tcagcaacac catttgctaa gtctaaagat gcaccaatat tacttactaa
     6181 aaataataac ttaaataaat taacagaaaa agaaataaat agattagaag caaaaaatgt
     6241 gtacatagtg ggtggattaa agtctgttga tgaaaaagta gtgtctgatt taaagaaaaa
     6301 aggattaaat gtaattagaa tatctggaaa tgatagatat gaaacatcaa taaaacttgc
     6361 aaaagaatta gacaaaaatt ctaacctgtc aaaagtagta gtggtaaatg gagaaaaggg
     6421 actagctgat gcagtaagta tgggagctat atcagcaaaa gaggaaatgc caattcttct
     6481 tactaatcaa aatgatgata tgaaagatat aaaagactta atagctaata aaaacatatc
     6541 aaaatcatat gtaataggtg gagaatcttt atttaataat aaagaagtga ataacacatt
     6601 accatctgta actaaaatag caggttctga tagaacagaa actaactcaa aagtaataag
     6661 tcatttctat agcaaagata cacttaatga cttatatgta gctaagaatg gaatgaataa
     6721 acaagacgat ttagtagatg ccttatcagt aggagtgctt gcaggtaaaa ctgaatctcc
     6781 agtagtatta gttggaaatg gattagatga tagccaaaaa gagttaatca agaataaaaa
     6841 gtttaaaaat ataactcaaa ttggtggaaa tggaaatgaa aaagcattta atgaagtaga
     6901 aaacttagtt aaatagattg aaaggtagta actggaaata gttactacct ccttttttat
     6961 tcattttcag ttttttcaat aattattttt ccatcctcaa aagtaacaag aacatccctt
     7021 tcatcaggag agatattcat ctctttaatc catgacattg gaagtatcaa tctgggtgta
     7081 aaagagcctt tgccactttt attgaatgca actctaagtt ttctttgttc caataatatc
     7141 attcctttct actattaata aatgccagca ataataaagt aaataccaaa gcaataagag
     7201 ctttaaaagt aaaacttatt attatattaa taatatttaa aactatagtg ataccaagaa
     7261 taatcataca aattctataa aatttattat tcatattaaa ttagaaaatg tgttataata
     7321 ttagaaagaa gctacctaga aggaaggtat tttcctccta ggattgaaac ttataattct
     7381 ttaatcaatc ttagtattgc gagtactaga ttaattaaaa ctaatgtgaa agagaaagca
     7441 tttttaattt tattaaattt gttttctctt ttattttttc ttctttctaa tctccccact
     7501 ttctttgtcc tcccttctaa gttttttatt ttcttacctc ctttctatat ttatattata
     7561 tcatcacgtg acgtgaaagt caataaatat tccatatttt tcaataaagc tataaaatat
     7621 attaatataa aatacaaaat ataataaaaa taagtggatg tttacattat acacccactt
     7681 attttagagt tattcctcat cagatttttt tattataatc tcatcttcct taaaaataac
     7741 tacagcctta cgtttttccc ttgtaaatcc cattttttca agttggctta aaggtaaaga
     7801 aattttagcc gaataagtac ctgaaccacc tttactgtaa actattttag attctttttg
     7861 ctccaaacta ttttcctcct tttttatatt gtaatgtaac aaatattaac aataaaatgc
     7921 ttactattaa tcctattata ttgttgatat taaaatgtaa aaataaaagt attatgtcta
     7981 ttactatagt tatagcaact ataataagac agaatttata aactttatca ctcatatttt
     8041 atagaaaatg tgttataata tttagaaaga agtcactagg aagaagatta aattcttcct
     8101 agcttacaaa actataaatc ttttaataat ttaattagtt tatcaattaa atttattaaa
     8161 atgggtaaga tagagagagc agttttaact ttggcgagtt tgctttctct atcttttttt
     8221 cttctttcta ttctactcat tttccttttc accccctttc tatatttata ttataatatg
     8281 ccgaaccggc aaaagcgata tttatttcca tatttttcaa taaagttata ttgacaaact
     8341 atattttttt acaaataaaa ataagggtgg caaaaccaaa cttgccaccc ttttgccacc
     8401 gaaaataaat aatagaataa atttaaatta aacataataa aacataaaca acaaaactga
     8461 ccttaataaa acattgaaat accaacactt gtaaaaaata acaacaataa acaaataacc
     8521 tagaataata ttacaactta agtaaataat aattttgaat aataatttac ggaggtattc
     8581 atatgtttaa acacgataaa gcattattaa aagaagtaaa agtagaaaga ccaaatcctc
     8641 aatatgctgt tttaatgcaa gaacagttgg gtggtgcaaa tggagagtta aaagcagcta
     8701 tgcaatactt gtcccaaagt tttagaataa aagaccctca aataaaagat ttatttcttg
     8761 atatagcagc agaagagctt agccatatgg aaatggtagc acaaactata aatttattaa
     8821 atggacatga tgttgattat aattcagtta atacaggcga aatagaaaca catgtattaa
     8881 ctggtctatc accagtctta ataaactcat caggagctcc ttggactgct aattacgtta
     8941 cagttacagg ggatttagta gctgacttac tttcaaatat agcttctgaa caaagagcta
     9001 aggtagttta tgagtacttg tatagacaaa tagatgataa atatgtaaaa gaaacaatag
     9061 atttcttact taatagagaa gaagcacata atgctctatt tagagatgct ttaaataaag
     9121 ttaaagatac aggttcaaat agagattttg gagttacaga agattctaaa ttatatttcg
     9181 atttgtctac tccagggcca aatcacgata ctaagataga tattaatcct ccttcttttg
     9241 aaaaaccttt aaaaaaataa tttgatattt tttatgttgt tttgttttag tagttataaa
     9301 aattatttac taggagaatt aattatatga aaaattaaat atgtatggtt aaaaatgggt
     9361 atacacttat tataagtgta tatctatttg tttattgcaa aacatataat aaaataacaa
     9421 atgaggtggt attttggaca gtaatagaaa acctaataat ctaataaatg aaaaatctcc
     9481 atatctttta cagcatgcat ataatcctat aaactggtat agttggaatg atgaggcttt
     9541 taaaaaagca aaagaagagg ataagccaat atttttaagt gtaggatatt caacatgtca
     9601 ttggtgccat gttatggaaa aagaatcttt tgaagatgaa gaagttgctg aaataatgaa
     9661 tagaaacttt gtggcgataa aagtagataa agaggaaaga cctgatgtag atagcgtgta
     9721 tatgacagtt tgccaagcca tgacaggtag tggtggatgg ccaatgacca taataatgac
     9781 accagataaa aaaccttttt ttgcgggaac atatttccca aaatattcaa ggtataatag
     9841 acctggagtt atagatttat tagagaatgt atctgaaaag tggaatacaa gcagggatat
     9901 attaataaaa tctggagatg aaataataga agctcttaag gatgattttg gtgtaaaaaa
     9961 tactgagggt gatttatcaa aagagatgtt gagttcatca gttagagtat ttaaggctat
    10021 ttatgatgaa aagtatggtg gttttggtaa tgctcctaaa tttccaagtc ctcagaatct
    10081 tatgttttta atgaaatatt atagtataga aaaagacaaa gatgtgttaa aaatggtgga
    10141 aaaaacttta gatggaatgt atagaggagg cttgtttgat catattggat ttgggttttc
    10201 aagatactct actgataaaa agtggttggc tcctcatttt gaaaaaatgc tatatgataa
    10261 tgctatgctt actatagctt ttttagatgc gtataaaata acaaagaaag agttatataa
    10321 agaaattgct ataaaaacta tagattatgt agttagagag atgaaggata aagaaggtgg
    10381 gttttattca gcacaggatg ctgatagtga aggtgaagaa ggtaaatttt atacatttaa
    10441 tcctcttgag ataattgaag ttttgggtga agaagatgga attttcttta ataattattt
    10501 tgatataact agtagtggaa attttgaagg caaaagtatt cctaatctta taaaaaataa
    10561 agaatatgag agacataatg aaaagatagc tgaccttagt aaaaaggtat ttgaatatag
    10621 aaaagaaaga acatctttgc ataaagatga caagatactt acatcatgga atgcattgat
    10681 gatagttgct ttaacaaagg catatagtac tttaaaaaat gacatatatt tagagtattc
    10741 aaataagtgt cttaacttta taaataataa tcttgtaaat gagtcaggta gattattagc
    10801 tagatataga gatggaagtt ccgactattt agcatattta gatgattatg cttttttaat
    10861 ctgggcgtat atagagcttt atgagtcaac atttaatatg aaatatttag aaaaggcttt
    10921 aaatttgaat gaaagctgta tcaacctttt ttgggattat gaaaaaagtg gattttatat
    10981 atatggtaaa gatagtgaaa atttaatagc aagaccaaaa gatttgtatg atggagctat
    11041 tccatcagga aattctgttc aattatacaa tcttataaga cttgcgaaaa ttactggaga
    11101 taatagactt gaagaaatgt catataaaca attaaaactg tatgtggata atgtaaagag
    11161 ttctccaact ggctacagct tttatatgct ttcattgatg tttgaacttt attctactaa
    11221 ggaaattata tgtattttta aggaagattc tgatttaatt gcatttaaag aattgataag
    11281 tgaaaatttt ataccaaatg caactttttt agcaaagaaa tacaatgaag aaaacactat
    11341 tataggtttt ttaaataact atatattgaa ggatgataag actagttatt acgtgtgtca
    11401 aagtaatagt tgtagtcaac caattaatga tttacaaaaa ttaaaagata tgattttagg
    11461 agtagaataa tcataatagc gaaaaaggag atgctttaaa ataaaagtct aatcatctcc
    11521 tttttttgta taaaaaacag aaaaagagtt cctaagaaca cctaatctat gtaatgattg
    11581 ttttgttaaa aaataaaaat gacattatag ttatataaaa tattgtacta taaaaaagtg
    11641 gtattataga tgcgatagta ttaatctttt ttatttaccc tatttcttct ttttgcaaat
    11701 tcttcaagtg cttcagattt aattttatct atttcttcat attccataaa attagcatct
    11761 tcttcaaagc catcttgttc ttcaccatta aaacgataca ttttttttag taaactccat
    11821 aaggtctcta attcatcttt ggtaaattcg tgaaatacat ctgccataaa gtttattcca
    11881 gtcctactac atgttaccat aacctttttt cctaagtctg taactttaac attaattgct
    11941 ctcttatcat gaggacttgg aattacatca acatatccat ttttttcaag attagcaact
    12001 agtctattta tattttgttt acttgtaccc atttttcttg caatattatt taatgttgtt
    12061 tcttcctctg gtaaatgaag aattgataaa atagtcatat actgtcttga agtaagtata
    12121 ccaaaatatt tatctccttc tatttgtatt ttgtttgtaa gggtaaaaag agttgcatat
    12181 gtttgattca ttaaaaagag ctcttttagt tcatttgaat aatccatgaa atatatctcc
    12241 tttattttta gatgttgttt tgtcgaaaac agaatatcat gaattaattc taatgtcaat
    12301 atggtgtcaa aatatattaa agtattactt ttttatgtaa ctattaatta attaaattat
    12361 tgtataattt tatgtaaaaa cttctatgta ccaatgataa tttctatgag gaattaataa
    12421 ttcttaaatt ttttagaaag attttgactt gaaaatactt gtaatataat atataaatta
    12481 atatactgaa taaaatcaat ctggagggat aattatgaag tgtacaaact ttactttcaa
    12541 aggagaggaa ggtttagata tatatacata taaatgggaa gatgaaaata taaaaaaacc
    12601 aaaggcagtt atccaaattg cacatggaat ggctgaaact gcacaaaggt atgagacttt
    12661 tgccaaggta cttactaaaa atggttatat agtatatatc aatgaccata gaggtcatgg
    12721 aaaaactgca aagataatag aaaatgttgg acatttagct gaaaaagaag gatttagatg
    12781 tcttgtagag gacatgtata ctttaacaaa tattataaag aaagaaaatg aagatttgcc
    12841 aatttatcta tttggacaca gcatgggttc atttgcaagt caaagatata taatggatta
    12901 cagtaataat ttatctggtc ttatactatg tggttcaaat ggaaagcaag gaatcattct
    12961 aaatcttgct catctgataa tcaatcgtga gattaaaaag tatggaagac gttctaaaag
    13021 taataaaata aataatttga tatttggtgg cgaaataata agaagaaatg aaaagactaa
    13081 gtttgactgg ttaagtagag ataaagaaca agttgaaaaa tatataaatg acccattttg
    13141 tggagtagtg tgtagttgtg gcttttttta tgatttagtt caagggttaa aagaaattga
    13201 ggataaagaa aacttaaaaa aagtgccact tgatatacct atttacataa tatctggaga
    13261 taaagacccc ataggaaaaa atggtaaagg agtcttaaga ctaagggata gatatataaa
    13321 attaggagta aaagatgtaa cttgcaaact ttacaaagat ggaaggcatg aattgttgaa
    13381 tgaaataaat agagaagaag tttttgagga tataatttgt tggttaaata ataaaataga
    13441 aatattgtaa taaaaaaata taatgatata tttgctttaa atttatttat atataatatt
    13501 tgaaaagtgg attatgtata tgcaaagtag aaaagagaga gcctagaagt gattttttgc
    13561 tataatgcca tttattgaat aggtgggtgt tattataaat aaattattaa cactcagtat
    13621 gattatcatt gtatatggaa tttattttat ttaaaattcc ttgaaagttt aaatatagta
    13681 agttgtaata aagagaaaat ctaaatattt aaatgctatt taatgtgaat ctattaatta
    13741 ggttatactt aaacttaatt aatagatttt atatttcaag ccaatgagat agggttttag
    13801 attaaaatat aaccaatggt taaattacag acattttgtt aactttccct cgatttacta
    13861 gtaaatattt tttttatata cttaaattac aacagatgta ctagggaggt atatatgcaa
    13921 attaaaatag gtaaagtcat acagtgtctt agaaaagaac gaaatttaac tcaagaacaa
    13981 ttagcaaagt ttataggtgt gtcaacacca gcagtatcta agtgggagag tggaaattct
    14041 tacccagata tagaattatt gcctttatta gcagactttt ttaatgtgtc tattgataag
    14101 cttttaaatt acaagattga tttaagtgaa gaagaagtaa tgaaaattta taaagaatta
    14161 gaatcaggct ttgctagaat tgaaattgat ttatcaacag aagaaccaaa agaagaattt
    14221 agacaagact tagaatctgt aaaaaagctt tccaatatgt atatagagaa gtatcctaaa
    14281 agctatttat tgaagctaag gatatgttcg ctgtatcaga tgtattcata taaatttggt
    14341 aagagtgaac ttaatgacag agtaaaagaa acaatgaata ttcttgaaga tatagtgaga
    14401 aatacagatg atatccaaat aaaagaaaca gctcttatta tattatcaaa cgcatattgt
    14461 atgttggagg attatgaaaa ggcagagttg tatttaaata tgatacataa gtcaataggg
    14521 gatacaagtg ttaatttagc tatgatatat ttaaagcaaa atagacttga agaagcagaa
    14581 atattgcttc aaaataaatt gtttagtaat gtatttaata taagcatgga ttgtaaggga
    14641 ataattaatg tatacaaaaa tcaatacaag gaattaaaga aaaaattgga gaatagaaat
    14701 tttaataaaa atgcaattga gagagaaatg gagtatataa aaaataagtt gctggggtat
    14761 gcaaatcttt ctttagaaat aaaaaaaatg ctcagtgaag ataaaggtgc attttttagt
    14821 atgtatatgg attatatgga attatctcta atttttttat tttttaacat gaaagaagaa
    14881 gcaaaaaaag ccttatatag tctaaaagaa atattagaaa aatatcctat acatgaaagt
    14941 ttggatgtaa gtcaaatgag attttttgat aaggtagagt ctaaaaattt atatactttt
    15001 aatatatata caaacttgtt aattgtactt aatgatgata gttataatga gcttagagaa
    15061 gaaccaattt ttaaggatgt aattgaaaag attttagata tggaaaaaat gttgaaaaat
    15121 aaagaataag taaaaattta atataggact taaagattaa tcaaattaaa agaaagtgtt
    15181 tcaaaataaa aatttgaatc accttttttg tcggtcttaa acagttattt agaaaaatag
    15241 aggtttcaaa atagaaatct gaattatctg tattattaca caaaaataga aacctaaatt
    15301 tataaaaata gaataaagtt tgttatataa aatgtaattt tgtctataaa atctcacttg
    15361 ttttcttaac tttaagcgtg aaaaagagaa tgtatcatga attttttagt tcatgatgca
    15421 tccttctttg tatgaaatcc aatatatctt tattatttaa acaatggaaa agagccttat
    15481 ttctattttg agacatctct tttatttaat tatgaagttt tatacagttc aataaatagg
    15541 aattatattt agaaactcat gacattattt cttagatagt gattaaattt atcatattcc
    15601 attttagata acttttctgg gtaagacatt acaatcaggt tatgcccatt aaatttttca
    15661 tggtatggtg gatgaatgtg ttcataatta tttgtcttat attttactct ttcatagaac
    15721 gccttgcgat aaattgaaat ctcatcaatt ggaaggtcaa tttctaaaat aatagttttt
    15781 cctttcttat ttaaaagctc taacgctctt tgtccatatc tattattgcg catttctgga
    15841 aaaatgcaga aatgttctac atagataaag tcatctgttt cccaacataa aattatgccg
    15901 acaaactggt ttttatcata aattaaattg aactgatatt ctttattttt caaaatttct
    15961 tcttgcaaag aagattttct ttgttcatga aatgggaaac taattttata taattccata
    16021 gccttgtaac ataatttatt ttcactggtt cttaaccgtt taaatttcac tttactcctc
    16081 ctaatcacga aaattccaat ttatttatga gttcatttta ccatagtcag tatttgcata
    16141 caatgtgttt taagttgagt gtcatttctt tcttttattt atgcttatat ctcatagact
    16201 aagagataat tgcttaagac ataactaaaa actattatat taagctttga taatctaaaa
    16261 acagtatatt tatggacttt tctaggtcta aaagtaatct taataatttt agaagttaca
    16321 aaaaagttac ataaatttgg aaagctataa tatacatata taatataagg ttaaaactgt
    16381 aatgaataat attaatttaa aatttgaaca tggaaggaga ttattatgga gaaaagagaa
    16441 aagaggataa taatatcatc tttattaagt gtatcaattt taatgggttt ggtaagtata
    16501 tattctatat taaataaaga agacataatt ttaactgtta agggtcaaga gcaaaaagta
    16561 tcttccttta agaaaacagt tgaggaactt ttggatgaac agggtataaa gtataattct
    16621 gaagataaga ttaatccaag tttagataca gaactaaaag atgatatgaa aataaaagtt
    16681 gttaaggtaa ctaaaagtaa aaaagaagag attgaaaaaa ttccatttga tacaaagcat
    16741 gtaaatgata gtaatttgtt aaaaggaaaa tctaaagttt atcaagaagg tcaagaagga
    16801 gaaaaaaaac tagtctataa tttaacttac catgatggaa agttagtcaa aaaagtctta
    16861 tcaaaagaag taatatctaa ggagccaact acaaaaatta taaaatatgg aactaaagaa
    16921 aaagtactaa tagcatcaag aggagcaaat ataagaggag gcaaacatat gaaagtggtt
    16981 gccactgcat atgcaggaga tacaataaca tctactggta caactccaag atggggtgtt
    17041 attgcagttg acccacgtgt aataccatat ggaacaaaag tatatatacc taaactgggt
    17101 atgacttttg tagcagaaga ttgtggaggt gcaattaaag gtaatagaat agacattttt
    17161 atgaacagtg aaggaaaagc ttcaaattgg ggaaggaaaa gtatagatat atatctacat
    17221 taaattagtg aatagaataa ctttacttga aggacaaaaa agtataaaat atgaattaac
    17281 taaactgtta gattattctt aaaacataaa aatatatcta aggggggata agatgaaaat
    17341 agtatgctta ggagacagtt taacgtatgg atttggagta tcacgaagta attcatggac
    17401 aaacattgta aataaagaga ctcgattaga aatagtaaat aagggtataa atggtgatac
    17461 aacaagtggt atgttagcta ggtttaatga agatgttgta aaaaattctc ctgatatagt
    17521 ttttataatg ggtggcacca atgattttat tgctggtgcg ggaaatgaaa ttattaattc
    17581 taatataatg gctatggtgc atcagtcata ttcaaaaaat attataccta taataggtat
    17641 accattaaaa ccagatattc caaatgttag ggaagattgg agttgtttta ctgattttaa
    17701 tatagtatct caaaacttag aatcatatag ttattggata aaaaaatttt gtatgacatt
    17761 taatactaat ttcgtagatt tttattcaga atataataaa aatatggaac tagaaggata
    17821 taaaaaatta tattttgatg ggttacatcc aaccaaagag ggtcatagaa taatggcaga
    17881 catttttatt aattcaatta ataagtatat agaagagtta tagattacat taaaatgctt
    17941 gtttatgcgt cagttgcaat aaatacatac agtagagagg ggtttattat ggattatact
    18001 aaaacttatg aagagtggat aaagggttca tattttgatg aagatacaaa gctagagttg
    18061 gagaatataa aaaataatga aaaagaaata gaggatagat tctataagga tttagaattt
    18121 gggactgcgg gtcttagagg aataattgaa gcaggaacga atagaataaa taagtacaca
    18181 gttagaagag ctacttttgg attggcaaac tatatattag aaaacacaac aaaagaagaa
    18241 acaagtagag gagtagtaat agctcatgat aatagacaca agtctagaca attctgtata
    18301 gaatctgcaa atactttggc agcatgtggt ataaaagcat atatatttga tagtttgaga
    18361 actactccag aattatcttt tgctgttaga agcttaaatg caatcgctgg tatagtcata
    18421 acagcaagcc ataatcctcc agaatataat ggatataagg tatactggga agatggagca
    18481 caagttatgc cagagattgc aaatgcaata acagagaaag taaatagtat acatgattat
    18541 agtacaatac caaccttaac ggaagctaat aaaaatttgg tagttttgtt ggacgaatca
    18601 caagatacta aatttataga agcagtgaaa agccaaatta taagaaaaga tttagttaaa
    18661 aatgtaggta aaagctttaa gatagtttat acacctcttt gtggaacagg gaatgttcca
    18721 ataagaagag cattgaaaga agttggtttt gaaaatataa ttgtagttcc agaagaagaa
    18781 aatccagacc caaattttgc agggcttgat tatccaaatc cagaagagaa aaaagcttta
    18841 aatagaggta tattactcgc aaaagaaaaa ggagcagacc ttgtaatagc aactgaccca
    18901 gattgtgata gagtaggtgt agcagtaaaa actactacag gtgagtatgc tttacttaca
    18961 ggaaatcaaa ttggtggtat gctgacacat tatataatag aaggattaaa agaaaataat
    19021 aaattaaaag aaaatcctac aataataaaa accatagtta catcagaatt tggagcagat
    19081 atagctaaag caaataatgt tgatgtatta aatgttctta caggttttaa atttattggt
    19141 gaaaaaataa aattatttga acaaaataaa aatagaagtt atgtatttgg atatgaagaa
    19201 agttatggtt acttagttgg gactcatgct agagataaag atggtgtagt ttcttcattg
    19261 ttaatatctg aaatggcagc attttactat tcaaaaggta tgagtttata tgatggttta
    19321 atagaacttt ataaaaagta tggatttttc aaagaacaga ctatatcttt gactttaaaa
    19381 ggtatagaag gtgtagaaaa gataaaagaa ataatatctt attttagaga aaatcaaatt
    19441 gattatataa atagtattaa agtagttgat aaaaaagatt ataaaaatgg tatagataat
    19501 cttcctaaat ctgatgtttt aaaatacttt ttggaagatg aatcttgggt agctataaga
    19561 ccatctggaa cagaaccaaa acttaaattt tacatagctg taaaaggagc tagtgatatt
    19621 gaagcagata aaaagattca aggtttgaaa aagtatatag atgacatggt cgaaaaatta
    19681 aaataaaata ttattatttt ataagataat tttgtaataa tatttattta gctgaaaatt
    19741 ataatttact ttcattgagt agaatatgga aatgttagta gaattgttat tttcagcttt
    19801 tatttgtgtt atttttactt aaataattat attatagaat gaatctgagt ataattaata
    19861 ttgtataatg aaattaattt gatagaaaag gggatattta tgtttggtat agataagaaa
    19921 ttgattgaat atataattgc aaaatccaat tacagtgaat taagttcaaa gtcagcagaa
    19981 gtatctttct tcttaatgtt gtccatattt ccttttttaa tatttacaat aagttctata
    20041 gcttatatac cgattcttca tttaaataag tatatagctt tgtttagaaa tatgatgcca
    20101 gaaggtgctt ttgctgtgtt atcttcaatc atagtttctg caatagacaa tagaaacctg
    20161 aaatttttag cagtcagttt tgtacttact atgtggacat tttcaagagc agtaaaagct
    20221 cttattaaag gaatgaatag agcctataaa gttaaagaaa ctaggtcttt ttttaaaatt
    20281 ttatctatat catttttatt tacaataatg cttttggtcc ttattttttt atctatgata
    20341 tttttagttt acggtgaaaa aataggttat tttattttta atcttgtagg attggatgag
    20401 atatttataa aaatatggga tattctaaga tacactgtag gtataataac aataatagtg
    20461 atatttacac ttttatataa atacactcca aataaaaagt taactattaa agaatctgcg
    20521 ccaggtgcta tttttgctac ttttgcttgg tttttagtat catttttata ttcttattat
    20581 acaaattact atgctaacta tgaagtaatc tatggaagta tagctgaaat aattgtactt
    20641 atgacctgga tgtattttag tagttggtct atcgttattg gctatgaggt aaattcaaga
    20701 ttgtatttta gaaagataag acatgaaatg ttaaagtaaa gtatttaaaa taaaagagag
    20761 tatttagtat tataaatgta attgaattaa taaagaaata ttgacattta agttaaaaag
    20821 tctagaattt ttctgggctt tttttatttt tagtataact tgacaaattt tatttaatgg
    20881 gatagtttta atttataatc atctttaaaa agatgactat tgataagtaa gagataaaat
    20941 tggtaagtat caaaaaatat actaaataaa aattatagtc ctaatgagtt aatttctgta
    21001 ttttcaaata atgcagtatt tagtcatgtg tttttatatg cgcctgtatt tctaattagc
    21061 aatatattag aataagctac tgtaattaga tttagaaaaa agcgacataa ataattataa
    21121 acaaataata aattacataa aattaaattt attcacaagt atatcttata aaatatgtta
    21181 taattaatag aaaattgtca tataattaat taagtagtat tattataaat aacattgaaa
    21241 ggtagtggtt agaatatgga tacccataat aaatatgtaa attttataaa aaatatacct
    21301 gtaccttttt tatattgtag aattgtaaag agacaagaag atatagaata tcgagtagaa
    21361 tatataagta agggtatggg caaagtatta caattagaag aaggtatttg tgataaaaat
    21421 atactagatg tattgcctgt atttaagtct aaaaaatatt tcaaagagtt gttctcaaat
    21481 gaagtggatt gtataaaaag gtacattccg acacttaaaa actggataaa tataaaaaaa
    21541 cagataatag gggattcata cataattttg tattttggta aaattgtatt tgattataga
    21601 cagataatcg attcctttga taaaaaggaa aaagtagctt atattaaaga cgaagaaggc
    21661 atatatatag attgtagtga aaatttaata ccaatattaa ataacaacat taaaacaacg
    21721 aaagatatct ttggtaaaaa tgatatagaa gtatggggag agaatacagg aaaattattt
    21781 agagatgatt atagagaagg agtatctagt aaaaaaagat ttttacaaaa tctttttgaa
    21841 tatgaagaaa cattttttat ggttgaaaaa tattttttgt atgatgaaga cgaactttta
    21901 ggtactatag ggatagtaga caatataata tatagtggtt acagtaatag aaattataac
    21961 tcaaaagatt taatgaagat gatagaacac tcaattccag agaatatgtt ttataaagat
    22021 gtgtatggaa attatattgg atttaattca ggttttttga atttagcttg tatgaataaa
    22081 gaagaattat tagggaagaa tagttataaa atatcagaag aagaagcttt aatagataag
    22141 atatttgaaa gtgataaggg tgtagttgaa aataaaaaag tagttacatt tgagttaaat
    22201 ataagtatga atgatgaaaa caagtgtatt gagattacaa aaaggccgtt ttttgatagc
    22261 tatggaagtg ttattggaat aattggaaca gcaagggata taagtagaag aaaaagatta
    22321 gaagaggaaa tggataaaac taggatggaa ttttttgcaa atttatccca tgaacttaga
    22381 acgcctataa atttaatatc atcttcatta caagtgatag aaaaaaaaga agcagattta
    22441 atagaatcaa atgatacttt aaaaagaaat ctaggtataa taaaacaaaa tggaaataga
    22501 atattgaggt tagtaaataa tgttatagac tttacaaaaa tgcaatcagg atatttagat
    22561 tttaaaccag aagaatctga tataattgca tttatagagg agatatgtat gtctgtagct
    22621 gattttgcaa gccaaaataa tatacaactt acatttgata cagaaataga agaattttca
    22681 atgttatttg attcagaaaa attggaaaga ataatattaa atttactttc taatggaatt
    22741 aagtacaata agaaagatgg aaaaataaat atatttttat atgtgaaaga taatgtattt
    22801 aatatgaaaa tatctgatag tggtataggt ataccaaaag aaaaaataga taaaatattt
    22861 aataggtttg aacagattga caatgaactt tcttacagag ttaaaggaag tggtattgga
    22921 ctttcattag tgaaatcttt ggttgagttg catgagggaa gtatatcttt aaaaagtcaa
    22981 cttggtattg gaagtgaatt tatagtttca ttgcctgtta gaagtaaaaa taatattgaa
    23041 aaatataatc ataaaagaga aatcagcaat gaactaagta aaaaattaga aatagagttc
    23101 tcggatttat aagtttaaat ataaaaaata aaattttaaa aaagtattga caattgattt
    23161 agaagggttt aaaataaatt acaacataat aaataaaaag gcgatgaata ggaagagtaa
    23221 atctaattcg attaccagag agaagagtcc gttggctgga agacttttta aattagaggg
    23281 attgaaaact acctaggagc tactttctga agtcatctgc attagtagat tttagtaaga
    23341 atagtcgtta gctgcgttaa agtgtaaagt gagttttcat gacatatgaa aactaaatta
    23401 ggtggaaccg cgggaataat actctcgtcc ttatgttata aggatgagag ttttttgtta
    23461 tttaaaaata taaacagtaa tcataataat tatagaaaat ataaaagcaa tgaataggaa
    23521 gagtaaattt aattcgacta tcagagagga aagttcacag gctgaaagac tttctaagtt
    23581 tgagaaattg aaaactacct aggagctgtg gtctgaagtt actttaaaaa gttttagtaa
    23641 gactaaccgt taactgcgtt aaagtgtaaa gtggatttta tcaataaatg ataaaatcaa
    23701 attgggtgga accgcgagta tagactcgtc cctttatata gggatgagtc tatttttatt
    23761 gcaataaaag attgaaaata aaaaaaaatc ggaaaattat aaatttagga ggaaaataaa
    23821 atggagaata aagtgaaaat tggagtacta ggatatggag ttgtaggaag tggacttata
    23881 gatataatag ataataataa agaaaaaaga aatatagaaa tagttggaat tttagttaat
    23941 aatttggaaa aacataagga taaaaaatat tctaatataa taactaataa tatagatgat
    24001 atatttaata aagatataga tatacttgtt gaagtcatgg gtggtttaga accatcatta
    24061 tcttatataa aaaaagcatt aaacaataaa attcatgttg ttacagcaaa taaagatttg
    24121 ttagctgaat gtggagatga gcttgcaaaa ttagcaagtg aaaataaagt aagtattaaa
    24181 tttgaagctt cagttgcagg aggtatacca gtattaaaac caataataga atcactagaa
    24241 ggaaataata tagatagtat aaatgcaatt ttaaatggaa ctactaattt tatattatct
    24301 aaaatgtatg atgagaatct atcttatgat atggcattaa gacaagctca agaattaggt
    24361 tttgctgaag caaatccaga gtcagatgta ttagggtatg atgctgctag aaaactatca
    24421 atattatcaa ctctagctta tgacaataga gtatattgga aggatgtgta tttagaagga
    24481 attacagata tagatgaaaa agatattgaa tatgcaaaaa aattaaattg taaaataaaa
    24541 ttgattggtc aaagtaagta tgaaaatgat aaagtaagtg cttttgttag acctgtttta
    24601 gtagaaaaag ataatatcct tgctagaata gacaatgagt tcaatgctgt catagtgaat
    24661 ggagattcag taggagaagt ttcttttgta ggtaaaggcg ctggaagttt agcaacagga
    24721 agtgcggttt attcagatgt tatagatata atagataata gagtttcaag tatagattct
    24781 tttactaaag ataaaataca agttaataaa atagttagag aaaagtgtgg agcacttctt
    24841 agatttaaaa aatgtaataa agatgaaatt ctaaatatag ttgagaattg tttagtaaaa
    24901 ttcgatattt taaatgatga tgatgaactt gctattatgg tttatgctga ttctgaatat
    24961 gaaataaaca acagtttatg tctaataaaa gataaaggat actgcgaaaa aatgaataaa
    25021 atgttgaaaa taagttaaaa aagaaaaaat ataagtttat atgtaacata ttttgacgat
    25081 tttaatacta tatagtatga gatgaaacaa aacatctcat aaaaaaatta aaattcaagg
    25141 gagagatatg ttatatggaa aataaaaaat atgcaaatgg tggttattca gaagactggt
    25201 atgaaagagg agaatctaca gctaaatggt tccagaatga tagagaagaa tatgaaagag
    25261 cagcatacga tgaagataga gaaagaagag gctcaaactg tggatgttca gattcaggag
    25321 aaaatagacc tagaaactgt gaaagattta gaagagaagc agagataaga gaaagagaag
    25381 caagagaagc attctgtgaa tcttcagaga gaaagaaaga agcactagca tatgaatgtg
    25441 aagctagaaa attatgggaa gaagcagaaa aatactggga tgaatattca aaatacaact
    25501 ataaaggaat cgaatattta gcagaagctg ctagattatt tgatgaaggt atggaatgtg
    25561 aagctagaag aaatggaaat aatggaggaa acaataataa ttgttgccat aaatgccata
    25621 aatgtaattg taactgctgt agaaaataaa aaaattaatt gacaaatcaa tagttaaaat
    25681 atatcatatt atgtataata aagagaaaat tcagaaaaaa ataatgatta gaagaaatat
    25741 aagtatattt attagacaaa tagtgctcag ttagtgaggt gagtacaata tgattaaact
    25801 cccatcctct agcttttgat agagggtggg agtttttatt tatttatata tcttaaatga
    25861 ggataatatt gtaaaataaa tgagtatgag attgtaaaat taggaggtaa aaaatgaaga
    25921 gtattaaaac atcaagtatg aaagaaaagg tagatgaaaa gatattaaga gagcaagtaa
    25981 gttctataat agaagatata agaaataata aggatgttgc acttaaaaaa tataatgaga
    26041 agtttgatag aaatacaaga gatgaattta gaattactaa ggaagaaata aaggaagctt
    26101 ataagcatgt agatgacgaa tttataaata acttaaagat tgctgccaaa aatataagag
    26161 aatttgctaa agctcaaaaa ctaagttttg aaaacccttt tgaaaaagaa atttatccag
    26221 gtgtaatctt aggtcaaact aatattccaa tagagtcatg tcttgcatat gttccaggag
    26281 gtcagtatcc attgttttca acagctctta tgcttataat acctgcaaaa gtagcaggag
    26341 taaaaagaat agtggcatgt tcaccaacta tgaaaaatac tgaaaagata aatcctaaga
    26401 cattggtagc tatggatata gcaggtgctg atgaaatata tgcaactgga ggagttcaag
    26461 ctatagcagc atttacatat ggaacagaaa agattaatcc agttgacata attgtaggac
    26521 caggaaataa gtttgtaact gaagctaaaa gacaatgtta tggacaagta gggattgatt
    26581 ttgtcgctgg gccaagtgaa gttcttataa tagcggatga aacttcaaat ccagtttata
    26641 ttgcagctga tttattagca caatgtgagc atgatttaaa tgcaagagga atattattga
    26701 caaatagctt ggaagtagca caaaaagttg aaaaaaatat agaaagtatg ctaaaagatt
    26761 tgccaacaaa ggacatagca tatagttcat gggaaaataa tggagagata atattggttg
    26821 atgatatgga agaagccatt aaaataagta atttttatgc accagaacat ttagaagttg
    26881 cagtaaatga gtataatgat atttgtgata gattaactaa ctatggttct ttatttattg
    26941 gaaatctttc tgctgaggtg tttggtgact atgtttcagg taccaatcat acattaccaa
    27001 cacttaaagc atcaagatat actggtggtg tttgggttgg aacttttatc aaaacttgta
    27061 caaagcagat atttaatgaa aaagctatac aatcattagc acctgtagct gaaaagctgg
    27121 caaaagaaga gggattatat gcacatgcaa aggctgctga ggttaggttt aaaaagtagt
    27181 aaaaaatttt attttggcag tcgagattta gtgttagact gagataagga aaacgctgag
    27241 aacccgaata aatactatat tttgtgggcc tcggcgtttc ttttatatct gtatataata
    27301 ggataatact acctgtgtgt ttaaatgcta gcttaatatt tcttttctaa ataatgttta
    27361 tgaaatagtt aatattttta gttaatactt gaaaaaaact tttgctaact gtgcaaattc
    27421 atctgcattc gtcaatatag cagggtgtcc tccctgacta aaaatgtgaa tacttgcttt
    27481 tgaaataaaa gttgccattt gtttgtactc ttcttcaaga ttatgacgac acatttcgtc
    27541 ttccttactt cctgtaaata aaattggcat ttctaattta cacaattctt tatgaaacaa
    27601 agggcgtttc tcgtttgcac attgcaatag agcttttgta tcaagattaa caacattctc
    27661 ccaatctttt ccatgacacc attcatagaa ttttcgtgct tgaatatctt gctttgattt
    27721 ttcccttcca gtgattagat tatctatgaa gttatcatta agagtacgtc catcaaagct
    27781 atctgctata attgcttcaa ctaaatcggg acgttctaat ccagtattaa cagctgccca
    27841 tgctcctcca cttgttccaa ttaagccaac tttaccacaa ttcaaatgtt caatcaatgc
    27901 tattgtctgt aaggcttcat catgccatat gtcaggagaa aattgagaaa ctctatccga
    27961 ctgtccatta cctaaaaaat caattaagat acatctaaaa ttttctacat ataatggcac
    28021 aagtgcttta aacatattag aagaaccagt attaccatgc aggaaaatta tcggtttacc
    28081 ttgtccatat tctttataaa aacaagaatt accttgatac tgaaaatatg acatatattt
    28141 tcctcctttt tatttgttaa gttaattata acaatatagg tatgaatgaa aaacctcatg
    28201 ttctttctta gaataaaaat gtgccacgca taaaacgtga cacatttcta attataaatt
    28261 ttgctgttta agtattcttt gtatgctttt ctctgtcaaa aagtatttct ctgataactg
    28321 cttgatactc attccatttt ggaaatttat acagatttga gaattacgag aagctaagac
    28381 ttgtttggtg tttgtgtttt caccccaatg ctttttgtgc tcttgctttt tgggaatata
    28441 aatcacttta ccatctatat attcttgcac ttgctcaatc agttctttag gcagtaagtc
    28501 aatcgaattt tgatagctca ttttgcacct cctaaataat tattctttag gattagcaaa
    28561 ggctactgcc actcacaatt aaaaataaaa ttttttatta tgtacaatag cctttgctat
    28621 gcacaagttc caattcccat aaactaaata ctcctttctg gtttgaaaaa ttcatctgaa
    28681 cattgatttc gcttttaaga tagagttcaa tgacaaattt acatttttat tatatatatt
    28741 cagatagata attgcaactg tctttgtatg aggatatttt attatagtca atactataat
    28801 atgtatttat atcatcaaac taatatgaac agttgaaagg aatatgaaca ataaaactta
    28861 gtcaggaaga ataattatga agcagacaat agagaaattt gattttaaag ttttattgaa
    28921 aatttattga caaatttaac ttgtaaatat tatactaaac ttaataaata aagtttataa
    28981 agtaaggtgt tgtctatgaa aaatatagtt ggaaaaccgc aaacgataaa aaaagtaaat
    29041 gaagatttaa taaaaaatat tataaaaaat gaaggtccta taacaaaacc agaaatttca
    29101 aacattactg aattgagttt agctacagtt aataagattg tagagcaatt agctttgaaa
    29161 aatgaagtta aagtaagtgg actaagtgaa tctactggag gaagaagagc tcaattattt
    29221 gaaataaatg caaacttaga acacataata gctttatatt attatagaaa ttgttgtata
    29281 ggggtagttg ccaatttatt aggtgaaata atttatcaag aagaatttaa tataagaatg
    29341 gatacatatg aaaacgttaa ccaagatact ttttttgtta tagatactct aataaaaaag
    29401 gaaaaaggaa acaatataag ggctattgga attggtgtcc caggagttgt aaaaaaaggt
    29461 gtagttaaaa atataccaac tataaaaagt tgggaaggta taaatttaga aaatttaata
    29521 aagagtaaat acaatactaa agtgtttata gaaaatgata caaatattac tactattggg
    29581 atttatcaaa aacagtataa agacaaatat aaaaatatgg ctcttatgta tttagaagat
    29641 ggtattggtt caggtatagt tataaatggt gaattgtatt taggttctac aaattttgct
    29701 ggagagttaa gttatctaaa tatagaattt ggtaagtata actctaacaa cttaaatctt
    29761 gaagattata ttatgagttt aagaaaaaga tatttagaaa gtaaagataa taaatttaag
    29821 aaagaaattt tatctataat atcaaacttt ataaggactc ttgtatgtgt cctaaatcca
    29881 caagctatga ttatacagtg tagtatatta accaagagtg acatggagtt tatagaaaaa
    29941 gatgtaagca ataatgttgg agaagaaaat tttcctaaat taataaaagt agagtcattg
    30001 agagaaggaa gtataaatgg agtaattagt atgtgtttaa aagaaactga ctatcaatat
    30061 tccttatcta ataaaaaagg aggatattaa tgtgaaatat ttggtttcaa tagatggagg
    30121 gggaacaaaa actaaatttt gtgtaagtga cttagatgga aacatactta aagaacatac
    30181 cactggttca actaattata agtcagttgg aattaaaaaa acatatgaaa atattaataa
    30241 tggatttaaa aaaatattaa aagatttata tatcgattat gacgatattg agtacactgt
    30301 atttggtata tctggttgtg attctcctaa tgattataag attataatgg atgaaatatt
    30361 aaaaattggg attaacaaag aaaaaatata tctggcaaat gatgcagttt tagcttttta
    30421 tgcacaagcg gattcccctg gtttagtaat tgtggctggg acaggttcaa ttatactggg
    30481 aattaaagaa gatggagaaa tatatagagt tggtggatgg gggtataatt ttagtgattt
    30541 gggttctgga tatgatatag ggagaaaatt attaaagaaa gtattgcttt attgtgatga
    30601 atgtcatgag tatagtgatt tatttagttg tgtcttggat ttctttggag caaatagttt
    30661 tgaacaactt gcatatatga ttactgatat aaacaataat gttgaaatag caaacttagc
    30721 ttcattagtg attgattgtg ccgaacaagg tgataaattg gctattgaaa tattaagaga
    30781 atcatcaact gaactatcta aattagctca agtaatattg agaaaaatat ctaatccaat
    30841 gaaagtggat aaaagcgaaa taaatatagt tttgtctggt ggtacattaa ataaaactgt
    30901 atatgctcaa atgttaatga ataatttaaa aacagaaaat acagataaaa atttaaaatt
    30961 tatatctcaa gaaaatcagc cagtgtatgg ggggataaga cttgctatgg cattagctaa
    31021 taggagggtc aagcatggat agaaaaaagg taatagtaca aatttttact ggaggtttta
    31081 aggctaaaga aataaagttt aatgatataa aatgtaaact actagaatta ttggactgta
    31141 ttgatataga aaaggttatc atgggatgga gtgtagatga agaattatat gtagaaacta
    31201 aaaaactatt aaataaatat aatgttgaac tatatctttg gttgccatgt ttttctgaaa
    31261 taggattact tgaaaaaagt aatttgctaa ttgattatat ggggaaggaa gtcaagggct
    31321 atgctttaca agaggatgaa aattttgagt tttattgccc aaataataag gaaaatataa
    31381 aaaatataaa gaaaatatat agtacttatt ttagtgatat taaatttgat ggaatttttt
    31441 tagataaaat aaggtatggt tctttttcga ataaactaaa tggtgttttt aattgttttt
    31501 gtaaagattg tttaaatata tatgaaaaaa ataatattga taaagagtcg ttaatgagag
    31561 aaatgaataa tgtgtctaag ggagttatag ataatgaaga tactccattt ggtataacag
    31621 aatacattaa tggaaaatac aaatttaaaa ataaaatatg ggaagatttt tttgaaacaa
    31681 aaaatcaaaa tatatacaag gcattattag agataagtaa ttattttaaa agtaagggat
    31741 tgaaaatagg tatagatact ttttctcctt ttatttctta ctttacaggt caagatttag
    31801 aaaaattaca agaagtagca gattttataa aaccaatgat gtatagaact acaaaagctc
    31861 cagctggttt accatttgaa tttgaaaggt ttattgatga atccatacat aagaataaag
    31921 agattggaaa aagaaatttc ttagatattt taggaattaa ggagtatgaa ggagatgtat
    31981 atcctataga atttgtacta gaggaaatcg attttatgac aagaaatctt aataaagata
    32041 tatatgtagg agttgaaata aataaaaagg aaattatatc tcctgttaca cctaagtaca
    32101 taagtgaaaa tatttcaaat ataaataaga caacagtaaa aggatatgtt ttatcttggg
    32161 atttgttgtc agcaccaaaa gaaaatattg atgaaattat aaaatggttt cggaattagt
    32221 gaggtgataa agtgagtaag atattggaaa tgcaacaaat atataaaagt tttggaaagg
    32281 tagatgtttt aaaagggata aattttgagt tagaaaaggg agaagtaaga gctcttttgg
    32341 gagctaatgg agcaggaaaa tctacactta ttaaaatttt aggaggagtt cattctcaaa
    32401 ctaaaggtaa agtaattttg actggtaatg aaataatgtt taaaaatcca catcattcta
    32461 aagaaaatgg tattagtata atttatcaag aattaagctt gataccaacg cttacagtac
    32521 ttgaaaatat ttttcttggt agagaaattt taaaaggaag tgtgttttta aacaagaaag
    32581 acatgcttga ggaatgtatg aaaatttgtg aggattttga ttttaatata gacccacata
    32641 tggtagtttc taatttaagt gtagcaaacc aacaaatggt tgagataatg aaagctgttt
    32701 catgtaattc tgaaattatc ataatggatg aacctacaac atcacttact aataatgaaa
    32761 aagatgggct atttaaaata ataagtagac taaagtctaa aggtaaaaca atagtctata
    32821 tttctcacat attagaggaa gtttttaaag tatgtgatag tgcaagtata atgaaaaatg
    32881 gaattatgat tggaacttat gatgtagagg atttaactaa attaaagata actcaactta
    32941 tgacaggaaa tgataatcac aaacttattg agaagaagga ttatacattt gcaaaatatg
    33001 aagagccacc agtactagaa gtaaagaata taagctggtc taacaaaata aaaaatgttt
    33061 catttaaagt ttatagaggc gaagttgtag gtcttgcagg tcttgtaggt tcaagaagaa
    33121 ctgaaattat aaatttgata tttggtatag ataataaaga tagtggagac atttttatca
    33181 atggtaaaaa agtagacata acttctccta aggttgctat aaaaaacaaa ataggattga
    33241 ttcctgaaga tagaaaaaat ctaggattaa ttttagaaca agctatatac acaaactcat
    33301 catcagtaca aattgataaa ttcaagaaat tcttgtttat agatagaaat aaagaaattg
    33361 agttttctaa aaaaggtgtt aaggaattag gaataaaagt taatgatgta aagcaagaag
    33421 taaaagagct tagtggtgga aatcaacaaa aagtagtagt ttctaagtgg ttagagaaag
    33481 atttagattt aataatatat gatgagccga caaagggaat agatattgct gcaaaagaag
    33541 atattttcaa tacaatcaaa gaattttcaa gtaaaggaat aggagtaata tttatatctt
    33601 ctgatttaga agaagtaata agagtaagtg atagaatttt agtaactaga gaaggttcta
    33661 ttgtagatga tttaaaaaat gagaatatac aggttcaaga tataatgaac agtatattta
    33721 atgtataaaa aatgaaaatg gaggactgaa atgaagaaag cttttgagaa gttaaattta
    33781 agagattatg gagtattatt aggtttttta gctttatgta tagctataag tattgcaact
    33841 ccatcatttt taggtaaaca aaatatactt aatcttttac gacaatcttc tataattgga
    33901 ataatttcag caggtatgac ttttgttata atatctggaa actttgatat atcagttgga
    33961 gcagtagcag ctcttgcagg tgctataaca atgaaatttg cgactggtgg aactaatttg
    34021 tttgcatcca tgctcttagg tgtagcgatt tgtgcagtaa taggattaat caatggtata
    34081 gtagttgcaa agataaatgt accatcatta atagccacta tggctatggt cactattgta
    34141 agaggattgc tgttgatgct tacaggagga tatccaatta ctgaaaatat accaatactt
    34201 gattacattg gaaatggata tttgtttaat ataccaatac cagtagtaat tttctttata
    34261 gtggtgttag cttcatttat tgtacttaat aaaactaagt ttggtagata tgtttattct
    34321 gtaggaggga atcaagaagc aagtaaactt aatggtataa atgtagattc tcacaaagtg
    34381 aaagtattta ttattaatgc tgtacttgca ggaatagctg gaatcgtact aacaggaaga
    34441 cttgggactg caactgcgat tgcaggtgaa ggatatgata tggatgcaat tgcatctgta
    34501 gtaataggag gtacatcagt ggcaggaggt tctggttctg tacttaaaac tgttatagga
    34561 gtattactta tgagtgttat aaacaacagt tttaatcttc ttggaataga tgtatatttc
    34621 cagtacatat ttaaaggatt aataatttta gcagcagttg gatttgattc ttatagcaag
    34681 aaaaaacttg cttcaagata aaaaataagg gggatttttt tatgaaaaaa ttaatgaaac
    34741 atttagcatt gttgctatca cttgttatga tatttggttt agttggttgt tcaaatggtg
    34801 gtgacaaaga aaaaaatgga gaaaagaaaa tagcagtatt actaccaggt tcaacaggtt
    34861 actttgtagc aactaaacaa ggtatagatg caaaatctaa agaattaggt gtaagcgtgg
    34921 aatatgcaga tgctcaatgg gatgctagta aacaattatc acaagctgaa gattttatgg
    34981 ctaaaggggt agacatgatt gcattatgtg gagttgattc tgctgtaagt gagagaatag
    35041 ttaaagctgc taatgattca gacgttccta tagtagcatt tacaaattct ataggaagta
    35101 acccaactgg agaatttaaa ggtttagtta catatatagg acaaaatgag gaagaaacag
    35161 gagcattaac tggaaaaatt gctaaaaact tattaggaga aactggtggg aaagctgtat
    35221 taatagaagg agtaccagga actactccac aagtattaag aaaaaaaggt cttgaaaaag
    35281 aattaaaaga tagtaatata gaaatagttt ataatcaaac ttcaaattgg gagaaagagc
    35341 aagctttaaa ggtaacagaa gatttaattc aaaagaaaac tgattttaat gttataatat
    35401 gtcaagatga taattctgct acaggtgcag gtcaagcttt aaaagatgct ggtcttaagg
    35461 ataaagttaa agtcataggt ttgggtggaa gtaaagatgg attaaaagct ataactgatg
    35521 ggttaataga tggtacaact tatatgtctg ctgtagaaga aggtggatta gtcatagaaa
    35581 aggcatctaa attccttaaa ggtgaaaaaa tagaacctgt tacacaaatt aaacaagttg
    35641 aagttaacaa agataatatt tcagagttca agggtgaatg gtaaattgtc atagataata
    35701 aataagattt ttagagttac tttagaaact atataaagta actctttttt taatttaatt
    35761 tagtgataaa ttattgaata gtatttctaa acaaataaaa aattaatata aacaaagact
    35821 taattatact aaattataag taaaactaat ataaacttat ggaaaaaagg aaaacgtcta
    35881 aagtattgga attactatag tgtaggtgtg gtattaaaaa aacataaaaa aacataaaaa
    35941 aattgttgaa aaaaatatag aaattttgta taattaaaga tttgaaaagt gcggtataca
    36001 aagccaattc ttttgtatat aatatatata aattcagaaa aaggaaggga tatgtatggc
    36061 caggcggtta tttttagaaa ggattggact tatattttta ggttcagcaa tattagcatt
    36121 tggagtttat aatttttatt atttaaacaa cataacagaa ggtggagtgt taggtatttt
    36181 actactgtta aaaaatttat tcaatataca accagcaata gcaaatgttg taatagatgg
    36241 tttgctactt ttagtaggat ataaattttt cggtaaaaaa ttccttatat attcaatagt
    36301 agcttctata actttttcag tgttatatga cttatttgaa gctataggac ctctagtacc
    36361 acaatcacaa aatatgcttt tatcaactat tttggcaggt gtaactgttg gaactggtgt
    36421 aggtatagta gtaaaagcag gttgtgcatc aggaggagac gatgctttag cattagtaat
    36481 ttctaagact acatctttga acatagggca agtttattta gcaacagatg ttattgtatt
    36541 gttgttatca ttattttatc tatcagcgtt tgatatcttt tattctttga ttgctgttac
    36601 aattagtggc aaggtaattg attttattta ttatcatggt aaaagtttgg atatggatat
    36661 aagtaatgat atagttccag aatgttaata taaattaaat aactttagat tttattaggg
    36721 gtggcagatt tgccaccttt tttgtgtcta aatctttata gaaagtataa tcctatctag
    36781 gtttttgcaa atctcaaata tagaattatg ttgaaatgac tgggaatacg attgctagat
    36841 gtgcatttta aaaacccaat tttatattta tgaaatacta atcattaatg gatgtttctt
    36901 aatcctatct ttatacctaa aatgataaga taaatgaagt agcaatacaa aactaaaatt
    36961 tgtaaaagag attaaattaa atggaggagc taaaatgttg gatatagtta tgatagcatt
    37021 gttattcata ggatttgttt ccatgaagtt acttgctgat tggtgtggga aacaaactga
    37081 aaaaaaataa tgtaatgtat ggagggatta cctatgtggt ttctggcagt tgtaattata
    37141 atgttaataa tatatttggt atatgcgctt attaatcctg aaaaatttta aacagtataa
    37201 ggctaataag taagttgaat tggggggaac aatttttatg gtgcagatag taatagtgtt
    37261 agcgattttt atgattttag taatacctat gggcaagtat ttatatcata tagctactaa
    37321 tcaaaaaaca tttggagata gactatttga taaagtggac aattttatat ataaggtatg
    37381 tagtatagat aagaaaaaag aaatgaactg gaaacagtat gcattagctt tattatttac
    37441 aaatgcagtt atggtattta taggatatat catacttaga actcaaagta tgcatatttt
    37501 taatccaagt ggaattaaaa gtatggaaca aggactatca tttaatacaa taataagttt
    37561 tatgacaaat acaaatcttc aacattattc aggtgaatca ggactttcat actttagtca
    37621 gatgacagtg ataatatata tgatgtttac atcagctgca acaggatatg cggctgctat
    37681 ggcatttgta agaggtctag tcggtaaaaa gaaaacattg ggaaattttt atgtagattt
    37741 aattaggata acaacaagag tacttttacc aggagcttta attattgggc ttatattagt
    37801 tacacaagga gtaccacaaa cttttgctgg gacagaaaca gtgactacta tagaaggaaa
    37861 gcttcaagat atagctagag gtcctgtggc agctcttgaa tcaataaaac atttaggaac
    37921 aaatggtggt ggattctttg gttcaaattc atcacaccca tttgaaaatc caacaataat
    37981 atcaaatata gtagaaatat tatctatgat gatattacca ggagcttgtg ttgttgcatt
    38041 tggtcacatg ataaagaata aaaaacaagg ttgggttgtg tttggcgcaa tgtcaatcat
    38101 atttttaata ggtctagtag tgtgctttaa agcagaaagt gcaggaaatc ctattttatc
    38161 acaattaggg cttaatcaaa gtatgggaag tatggaaggt aaggaagtta gatttggtat
    38221 agcacaatca tcattattca caacagtgac tacttccttt acgacaggta cagttaataa
    38281 tatgcatgat acactgactc ctttaggagg attagtacca cttttaaata tgatgctaaa
    38341 tgtagtattt ggtggtaaag gcgtaggtct gatgaatatg ttgatgtacg ctataatagc
    38401 agtattctta tgtggattaa tggtaggaag aacaccagag tttttaacta agaaaatcga
    38461 aggaaaagaa atgaagctta ttgctttact tattatatta catccacttt taatactaat
    38521 gttctctggt ctttcagtag caataccagc aggacttgaa ggaatttcaa atcctggatt
    38581 ccatggatta tcacaggtat tgtatgagtt tgcatcttca gcagccaata atggttcagg
    38641 atttgaaggt ctaggagata acactatgtt ttggaatata acaacaggta tagttatgtt
    38701 ctttggaaga tatgtgtcta ttatagtact actagcaata tcaagtttgt tagcatctaa
    38761 aaaagctgta aatgaaagta taggaacact tagaacggat aattttacat ttacaatagt
    38821 tttagtactt gttgttttaa ttgttggtgc tttaacattt ttcccagcct tagcgcttgg
    38881 acctatttcg gagcatctag tgttgtggca ctaaaaaatt gggagggagt aaagttttat
    38941 ggaaactaaa aaaacaaagt ttattacaaa agatattttc aaaacttcca ttatagaagc
    39001 ttttaaaaaa ttaaatccta aatatatgat gaaaaatcca gttatgtttg ttgtagaaat
    39061 tggatttata gtaacattga tacttacaat agtacctagt ttgtttggtg atgaaggcaa
    39121 taacttaaga gcttataatg ggattgtaac aataatctta tttataacag tcctttttgc
    39181 aaattttgct gaagctgtag ctgaaggaag aggtaaagct caagcagata cacttaagaa
    39241 aactagaaaa gatacagtag caagggttat agatacgtta ggaaatgaaa aaatgataag
    39301 tgcaagtgag ctaaaacttg gtgatattgt tttagtaaat gctggagaag ttatcccaaa
    39361 tgatggagaa gttatagagg gtatagcttc tgtggatgag tcagcaataa ctggagaatc
    39421 tgcacctgtc atgagagaat caggtggaga tttcgcatct gttacaggtg gtactacagt
    39481 tgtaagtgac tggttaaaga taagaataac aacaaaacca ggagaatcgt tcttagataa
    39541 aatgatagct cttgtagaag gtgcttcaag acaaaaaacg ccaaatgaga ttgctttaaa
    39601 tactttgtta gtagggttaa cacttatatt tatggttgtt ttaatatctc tttatccaat
    39661 ggcaaactat gcaggtgtaa agattccaat ttctactatg atagctctct tggtatgttt
    39721 aataccaact actataggtg gattgttgtc agctattggt attgcaggta tggatagagt
    39781 aactagattt aatgtaattg caatgtcagg aaaagcagta gaagcttgtg gagacgttga
    39841 tactatgatt ttggataaga caggtacgat tacttatggg aatagacttg catcagaatt
    39901 tatagcagtt ggaaatgcag attcaaaaga tttaataaat tattctgtta tgtgttcatt
    39961 aaaagatgat acaccagaag gaaaatctat agttgattta ggtatgaaat taggttcaac
    40021 aggtaagaca aaagaggcag aggaagctga gtttgtagaa ttttctgcac aaactagaat
    40081 gagtggtatt aacttagctg atggtagagc tataagaaaa ggtgcttatg atagcataat
    40141 aaaaagagta aaagaatctg gtggtacagt gccagaagat ttagaagaaa atgtaaatcg
    40201 agtagctaaa ctaggaggga cacctttagt agtgtgtgta gataatgaga tatatggaat
    40261 tatctactta aaagatacag taaaatcagg gcttgtagaa agatttgcta gacttcgaga
    40321 gataggaata aagacaataa tgtgtacagg ggataatccc ttgacagcag ctacaattgc
    40381 aagagaagct ggagtagatg gatttattgc agaatgtaaa ccagaagata aaatagatgc
    40441 aataaaaaaa gaacaattag agggtaaaat agttgcaatg acaggtgatg gaacaaatga
    40501 tgctcctgca cttgctcagg cagatgtagg tcttgcaatg aatagtggta caacatctgc
    40561 aaaagaagca gcaaatatgg tggatttaga ttcagaccca actaagatat tggaagttgt
    40621 tgaaatagga aaacagcttt taataactcg tggggctttg acaacattta gtatagcaaa
    40681 tgatgttgca aaatattttg cgattatacc agctatattt actcttgcaa tacctcaaat
    40741 ggagatatta aatgtaatga aactttctac gtcatttagt gcgatattat cagctttaat
    40801 atttaatgct ataattatcc catgtttaat acctcttgca atgaagggag taaaatatag
    40861 acctatgaaa tcacaagcta tgttaatgag aaatatgctt atatttggtc ttggtggagt
    40921 tatagtacca tttttaggaa taaaattaat tgatttattg attacacctt tagtaatggt
    40981 tcttaattta ggttaataaa tggaggatat aaaaatgaaa acattaaagc cagcattact
    41041 tgtaagtatt gtattgttag ttgtttgtgg acttgtatat ccacttgttt tgacaggtgt
    41101 aagccaagtt gcatttaaag ataaagctaa tggaagtatg atagaagtaa atggtgtaaa
    41161 agtaggttca gaacttattg gtcaaagctt tacagatgcg agattcttta agggaagagt
    41221 atcatcagta aactataata cttatacaaa agaagattta gtaccagata aagatggaaa
    41281 cacatcttat ggaggtgtat catcagggtc ttttaattat ggagctacaa atccagaact
    41341 tcatgataga gtacaaaaag atatagaaca gtttttaaag gataatccaa ctgtcaaaag
    41401 agaggatata ccaacagatt tacttacagc atcaggttca ggacttgacc ctaatataag
    41461 tccagcctct gcaaaaattc agataccagc tatagcaaaa gcgtctggaa tatcagaaag
    41521 taaattacaa aaaattgtag aagataatac ttccaaaaaa ctttttggag ttttaggtga
    41581 agatagagtt aatgtattaa aggttaatgt agaggtcgca aaaatattag gtcttatata
    41641 gtattttgtg gaattttagc aacaaaatat tactcaagat ttaatttaaa ggttaaattt
    41701 taaagagtga aaatcgataa tgattttcac ttttttattt gaatattact aatatttgga
    41761 ataaagtgat tgtaagtata aaatccaaaa aaaggttgca ttatcaaaat gataatacta
    41821 taatatattt atcgttatca atttgataat agatttggac atataggagg aaatagtatg
    41881 ttatataata acgcattaga gttgatagga aacacacctg tagtaaggct gaataattta
    41941 ggctgtacta atgggtgttc taacatatat gtaaaattag aaaaagtaaa tccagcagga
    42001 agtataaaag atagagctgt ttatgaaatg ttagagggtt tagagcaaag aggagaatta
    42061 aaaaagggag atgtactagt tgaagcaact agtggaaata ctggtattgc attatctatg
    42121 ataggaaaat taaaaggata tgaagtagta atcgtaatgc ccgaaacaat gagcgttgag
    42181 agaagagatt taatgaaggc ttatggagca aatttggtgc ttacagatgg taaagctgga
    42241 atggcaggtt ccatagaaaa agcaaatgaa cttttaaaag aaaatccaaa ctataaaagc
    42301 ttaaaacaat ttgaaaataa agataatcca aatgcacatt ataaaacaac agcagttgaa
    42361 attatgaatg atgtcaaaga tttagatata ttcgtatgtg gagttggaac aggtggtact
    42421 ttaataggaa ctgcaagata tttaaaagaa caaaatccaa atataagagt aattgcactt
    42481 gaaccaaaaa aatctccagc tatatcagaa aacaagactg gacctcataa aatacaagga
    42541 ataggtgcta actttgttcc tgaaaattat gattctagtg tagttgatga agtaatatta
    42601 gtagatgatg aagatgcatt tgatacagtt aaattacttg cagcaaaaga gggaatttta
    42661 gtagggatat cttctggagc aaatatattt ggtgctatag agatggcaaa aaagtatcca
    42721 gataaaaaga tagttacagt agctccagat ggagttgata agtatatgtc tatgggaata
    42781 ttttaataat tttatttgag ggagtgaata ctttgtttaa gaaaataaat aaagatattg
    42841 aatatataat gaagaatgat ccagcagcta ggtctaagat agaagttttt ttactttatc
    42901 catcagttca tgcaatgata atgcatagga tggctcatgc tctttataag aagaagaagc
    42961 tttttacagc aagattaata tcccagatat ccagatttat gactggcata gaaatacatc
    43021 ctggtgctaa aatgggggaa ggtatattga tagaccatgg aatgggtgtt gtaattggag
    43081 agacagctga agtaggtaat agggtgacaa tatatcaagg agctacttta ggagctacag
    43141 gaaaagatac tggaaagaga catccaactg ttggtgatga tgtattaatt ggtgctggaa
    43201 caaaaatact aggaccttta aatataggtt caaattcaaa aattggtgca aattctgttg
    43261 tcgttaagga tgtacctaat ggtgcaacag ttgttggtat accagcaaaa atagtaaaaa
    43321 taagaaacct agaacctgta aaaaagaata aaaaagaagt gtcctatgag tatgatgaat
    43381 tagataatgt atattatata taggatttat ataactataa aataaacttt ataaaaatta
    43441 ttttaaaagg tatttttttc aaatttgagg acataataaa aaagaactga atgttacagt
    43501 tttggttcat caatctttga taaacaagat tttaaatttg tagcgtctag gaggatttta
    43561 ttatgaaagc aaatgttaat caagatactt gtataggttg tggattatgt ccatcaatat
    43621 gtccagaagt atttgatatg aaggatgatg ggaaatcaca tgttatagtt gatgaagtac
    43681 caagtgatgc tgaagaatca gcagcagaag ctagagaaag ctgtccagtt gatgcaatag
    43741 atgtgcatta ataaaataaa tgcctataaa aattgaattt tcaattttta taggcatttg
    43801 tcttttattc agttagtatt tcaacacctg tttctgtaac taaaaccata cattcccatt
    43861 gagcagataa tttaccatct tttgtataaa tagtccaatt attttcttca tcaacaaaaa
    43921 tttcgtgagt tccttcattt accataggtt ctattgtgaa tatcatccca ggaaccaata
    43981 gcatctcagt ccctttttta gatacataac ttacaaaagg gtcttcatga aagcttaatc
    44041 caactccatg accacctatt tctctaacaa cagagtatcc atttttcttt gcataagagt
    44101 tgataacatc acctatatct cctaagaatc cccatggttt ggcaacttca agaccttttt
    44161 ggacacattc ctttgtaact tgaactaatt tttttgcatt ttcgttaaca ttacctatca
    44221 taaacattct tgaagcatct gaatagtatc cgttaaaaat agtagaaaca tctacattta
    44281 taatatctcc atcttttaag ataatgtctt tacttggaat accatgacaa acttcattat
    44341 ttactgatgt acatacactt ttaggaaatc cattatagtc tagtggagca gggatagctc
    44401 cattttgtag tgtaaagtca tgaaccaact tatttatatc ttctgtagac attccaattt
    44461 cgattttatc tgaaatataa tccaaaattt ttgtgtttat tacagcactt ttttttattt
    44521 cctcaatttg actttcattt tttataatat catgagttgg tacaatatga cctaacttag
    44581 caaatgaatc tattttatta tccatatcca tatgacattt tttatatttt ttagtacttc
    44641 cacaccaaca gctatcattt ctatttaact tcataacttt tcatccttct tttattattt
    44701 atttaaatta tgggttacat aaactatttt aaaacataac cattataaat attataacat
    44761 aaatactaag aaatccttta tagatagtga tttattcaaa gtataaatta ttatatggtt
    44821 attgttgatg aaaagaggat agttaaatgt attattggat attagcgcac ttaatagttt
    44881 agttcaagtt tttcaagaaa atcatatgtt agaggacaat tttcctttaa agtatttttt
    44941 gaatcttcat caagataata atgtacaaat gattctgcaa aaaattcttc tataaacttg
    45001 cttatatatg catgtgcaac ttgcttaggg tcataaagct tatatctttc ctgagcaaat
    45061 acttccataa attcttcaga atttgatttt ttatttaaaa catttttatc tatagcatgt
    45121 gcagtttcat gtaattctaa attttttgat gaatgtacat ttgcatagct agggttacta
    45181 taacctattt tagccactat agaattattt ctatatatac caggtacatc tttccaagtc
    45241 ttgcctgatc ttacccaaca tggaggtaat tgatatttta aatcactaaa ttctggttca
    45301 tcagttagat ttgaatttat taattttatt tttatatcct tttctttaag tgtctgtaaa
    45361 tatttaatat ttatttttgc aagtctgttt ttcatatttt caacttcatc tttatcataa
    45421 ttcaagtcat caggaagtat aagcatttgt tcaattatat ctatactact gtcttttata
    45481 ttttcagatt cttcatcaaa tttatattga agagtattat tttcatagat agttatattt
    45541 gtattatttt ctatagaata agagtgaata tcctctaaat ctaaaatatt catttgttgt
    45601 aaaatatgtt ttgtttcgcc actccacact actaaagaaa gtgaattaaa attaattagt
    45661 ggattttctt ttgcttttat atcattgctt atatctacat caaatacata ttcaattgag
    45721 tcttctatgt taagactatc tgtattcttt ttaatatata aatacgcttg ttttaagtct
    45781 atataaatct catctaaaga ttcgtcataa aattctgata aattattagt aaagagttct
    45841 tcacttaaaa gttcattttt atcagttata ctttcaatta aaatattaat ttcttttatc
    45901 atattgtaaa tacttatttt tctatttgct ttttcactag atacattgca tggcgcaaga
    45961 gcaatgcaat aaaaatcttt ttctttttta taaaaggctt taagtgattt tgataaaagt
    46021 gtaaagtcta cacctatctg taagttgacc ttaagtgagt cattttgtaa taattcagta
    46081 ttatttcttt tagctgtatt taaattgttt tccataatca ttgccccctt tttattaggg
    46141 tatcataatt tttttatatg tgtcaatttg tttttttgaa aattataatt tttttgaaaa
    46201 ttttattttt tataatttgt gaatttttta ttatagaaat aaatatgtta ttattatgtc
    46261 aaaatttagc attagattca tgtagagtat gttttataca tatattataa ttaattcttt
    46321 gaagattgca tattcaaaat acacaccatt ttttttaatt ctataatata atatttatat
    46381 atttaatgtt aggatgtgat ttaaggtgta ttttacatat attataagat gtaaagatga
    46441 ttctctatac actggatata ccagtaatat agttagaaga atgaatgaac ataaattagg
    46501 aattaattca aaatacacaa gagcaaaggg atttgaaaaa ctagaagttt attttgtgac
    46561 caacacaaaa agcaatgcta tgaagttaga atattatata aaaaaattaa ctagaaataa
    46621 aaaattgtct ataataaaaa acccaagtat acttataaat ttaattgata ataaagaaga
    46681 ttatattatt ggtaaggaaa tagaacaatt aacataaata tttatagtaa cattataatg
    46741 ggtgcatatg gtgtaaaaac ttgacaataa atactattgt aatataatga agttataaat
    46801 atagtaaatt aaaatagcta ggggagccaa gagctgagag gtataccgac ccttacacct
    46861 gatctggata ataccagcgt aggaaagctt agtaaaaatt caagtatgta ttatttgtga
    46921 tatatgctat ttctaagcta tacctattgt aggtatagct tttttaatta agttatttaa
    46981 attaaacttt aggaggcaac aatgagtaat tataaaatac caacacttac aatagccggc
    47041 tctgattcat ctggaggagc tgggattcag gctgatttga aaacgtttag tgcaattggt
    47101 acttatggta tgagtgtaat aacagctata acagcacaga atactaaagg agtttttgct
    47161 gtagaggatt taaataagaa aataataaaa aaacaaatag aggcagtctt tgaagatata
    47221 cctccaagag ctgtaaaaat aggaatggtt tcaagtcctg agattatact tgaaatagtt
    47281 gagaatctaa agaaatacaa tccaaaatat ttggttgtgg acccagtaat gatatctaaa
    47341 agtggatatt atctattaaa gccagaggca aaagaaaact taattaagta tttgatacca
    47401 cttgcataca taattacacc aaatatacca gaggcagaag aaataacagg aataaaaata
    47461 cataatgtag atgatatgaa gagagtagga gaagaaatac tacaacttgg acctaaattt
    47521 gtacttatga aagggggtca tttagatgga gaagcagtgg atattttagt tggtaaaaat
    47581 atatttaaag tttataaaag tgaaagaatt gataaaaaaa atacacatgg aacagggtgc
    47641 acattatctt ctgcaataac atcttatcta gcattaggat atgaaataac agaagcagta
    47701 aatctaagta aaatctatat aacagaagca ataaaaagaa gttttgatat aggtcatgga
    47761 gtaggaccag tacatcattt ttataaattt gagtaataaa aaaatattgg aggtagaata
    47821 tgtataattt aattaaagat gtaaaaaaat taaatccatt ggtaattcat tatactaata
    47881 atgtaactat aaatgattgt gctaatgtaa ctttagctgt aggagcaagt cctcttatga
    47941 gtttttcata tgaggaagta gaagaaatgg taagtgtagc aaactctgta gtaataaata
    48001 taggaactat gaactcaaat atgttggatt tattcctatt agctggaaaa gcagcaaata
    48061 agtacaataa acctgttgtt ttggacccag ttggagtatt tgcaagcaaa gcgagagcag
    48121 aacttacaag tagactttta aatgaagtca aatttagtgt tgttaaaggg aatgtatcag
    48181 aaataaagtt tataggtggc tttaatgtaa ggggtaaagg tgtagattca tttgatgaag
    48241 aagaagattc tactgagata ataagaaaga ttgctgaaaa gcttgaatgt gtagttgtag
    48301 caactggaaa aatagatata ataactaatg gtaaaggtac atataaaata aacaatggaa
    48361 ctgataaatt aaaaggaatt acaggaactg gatgtatgac tgctagcttg attgcaagtt
    48421 ttatggctgt tactgagaat atattagaag cagcaacaat gggtgttctt acaatgtctt
    48481 taagtgggga attagctaat ttaaataatc caccaatagg tacatttaaa gaaaatctta
    48541 tgaatgctat ttatcaaatg gatatagata ctttaagtaa aaattctaat attgaatttt
    48601 taaattaata aagagtaagg gagacttaaa atgattgata aagaatcttt aaaaaaatgt
    48661 ttaaagttgt atttagtaac tgactctgaa atgcttaaag gtagagactt ttataaatgt
    48721 cttgaagatg caatatcttc tggaataaca actgtacaac ttagagaaaa aaatgcttct
    48781 ggaagagagt ttttaagaaa agctatgaaa cttagagaaa taactaaaca atatggtgtt
    48841 aagtttataa taaatgatag agtggatata gcacttattt gtgatgcaga tggagtacat
    48901 gtaggacaga gtgacattga tgttagagag gttagaaagc ttataggaaa caataaaatt
    48961 ctaggagtat cagcaagaac tttagaagaa gctatttgtg caaaaaatga tggcgcagac
    49021 tatttaggtg tagggtctat attcacaaca tctactaaat tagatgcaaa atcagcttcg
    49081 tttgaaactg ttaaagaaat taaagaaaaa gtagacatgc catttgtact aattggaggt
    49141 ataaatttag acaatataga taaacttaaa tgtttagaaa gtgacgggta tgctataata
    49201 tcagctatat taaaagctga agatatctct aaagaagtgg aaaagtggac attaaaaata
    49261 taaaattggc tctggtagtg aaaaaaatgg aaaaatttga tataatctaa gagtaaaatt
    49321 taagcaatta taaatgataa acttttctac atatactttt taatcaagat atatgtagat
    49381 ttggttaaaa aggagctata aatgattagg atattgttag tgtgtgttgg aggcatgtct
    49441 tctaccctat tggtaaataa aatggagaag gatgcaaaaa aaagaaatat agactgtaaa
    49501 atatgggcag ttggagaagg agatataaaa tcggagcttg ataactttga tattttactt
    49561 cttggaccac aacttagatt tatgcttgat gacgtaaagt ctatagttgg agatagagct
    49621 ccagtatcta taatagatat ggtaaactat ggcacttgta atggtcatgc tgtgttaaat
    49681 tcggttttag aaatattaaa ataaataata aaagtggatt ctatataata gagtccattt
    49741 ttattatttg ttattaatag gcttaaaact tagcttacca tttctttttt taatctccac
    49801 atagcaagtg aaaaacttga acagcacaat attaaaaata gtaggataac aggcagtata
    49861 tctccaataa gaatagtctc accaaatatt aagctacgta aagcgttaat aacatgggta
    49921 aatggattga gcatcactgc tacttttaat cctccagata aactttctat agggaaaaga
    49981 gctgaactta aaaagaaaat aggaagtaca attgcagtca taattgtttc ataaactatt
    50041 tcatttggca ataataaact tatagaataa gctagacttg ataaaaagaa ggctgtcata
    50101 aatatcagta ctatcataag taggattcca ccaatgccag attctatcct tactgagaag
    50161 aatatactta ctatacacaa aatagttact tctatgaaag aaactaagat agcttctaat
    50221 aattgaccaa ggacgattga atacctactg ataggtgcaa ttaagactct atagaaactt
    50281 ccactgttct tcattataaa gtttataata ccaccactac tacaagaaga aaacacaacc
    50341 agtacaatga tacctggtag tataaaagca gtataattat tgatatttat attattcata
    50401 gtttggtttg caatagaact ataaagcaca agccaaagaa aaggttgtaa tatagtaact
    50461 acaaatgaga ttggattttt aaaacgccac ttcatactac gccataagat atttaacatt
    50521 cccattcctc ctttaagctt tttttagaac ttgtaagtgc taaaaataca tcttcgagac
    50581 ttggctctac aatttctatt gcatcaaatt ctatatcatg ttctaaaagc catttattta
    50641 ttaaagtaaa agcagttcta ctatcgttta cacttataaa aattgaattt tctcgtacgc
    50701 tcataaactt tactaaacca gtacttttaa ttgaatcttt atattttttt atatcttcag
    50761 tattatgaaa agttatacgt agtatatttt gtctaatgta actacgtaaa cttgatggtg
    50821 ttccttgtgc taaatccttt ccattcttca taatacaaat attatcactc aattgttcag
    50881 cttcttcaag ataatgagta gttagaaata tagttgtgcc atactcatct ctaattttta
    50941 ataacatatc ccacatagat tttcttgaat cgacatccat accaacagtt ggttcatcta
    51001 aaaataggat tttaggagat gataccatat tcatagctat gtccaatcta cgttttacac
    51061 cccctgaata tgatgaagta ggatatttta agtaactaga taagtcaaac ttatcaatta
    51121 aagagtctat tctttgtttt gcaatctgtg gttctacttt atacatttta ctttgaaata
    51181 ccatattttc cataagggat aaatgttcat caatagagat ttgctgtgct acacaagcaa
    51241 tttgagtacg tatccaagaa ggattatcta ctaaatcttt tccaaacatt gttacatttc
    51301 cagatgtagg tttgtaaaag gtagtcaaaa tatttataag agaagattta ccagcaccat
    51361 taggaccaag taaagaaaaa atttctccag catttacatt aaagcttaga ccatttaaag
    51421 ctttcacacc atttttatat tcttttacta aattgtctat ttcaatcgct aacatatatt
    51481 attcctcctc tttaagtgga aatattatct ctgtaatata gttatctggg tttcctttta
    51541 tcaacatacc tggtccttta ataaatactt ctctccaagg agtttgtatt tttatattat
    51601 tttcatgaat atatttaaat atcatttcat acgtttttgg caaattacta taagagcctg
    51661 tgtgagtaaa aaataaagct tttgttttag gaaattcttt tgtagttata cccatacgat
    51721 ttggagtttc agcagttgga acacataatt ccatttctgc aacaccagtt ttttggtcaa
    51781 catcatagta gcaaaagaat ggtgcaccgt tagactttcc ttttatagct ttaaatacat
    51841 ttggaaaata ctttgttgcc tcagtcatag gtccattata acgcatggta gccactgtga
    51901 ctgattcgat atttttgatt tcaattttgc aattcatttg tttacctcct aatgtgtgtt
    51961 catattctta ctagattttt aagcattaat acatataaga ttttgattag ttgaaaacat
    52021 ataatcttat ctcgtttgct tgttttcaag tctaacataa cataaaatat atatcctctc
    52081 atttgatgct ccattttgta aagttattat ttaagcttta ctttagattt tttagcaaat
    52141 taaaataaag caatttataa aataagtatt tacaaattaa gtgtattaat cgtataatac
    52201 acttaatagg atactagata aagtctgaaa taaaggcgta tttttttaaa gtaagtgtac
    52261 tatcgtatta atacactaga ataggaggaa tcatgaattt tgaattagat aatacaactc
    52321 ccatttattt gcaaatagta aagtatataa agagacaaat agtaacagga gagttgaaac
    52381 caggagagac aataccatca agaagagaaa tggcacttaa tctaaaagta aacttaaata
    52441 cagttcaaag agcatataag gagatgggtg atatgaatat aataaatact tttaaaaatt
    52501 atcaaagcag tgtaacagtt gatgaaaata tattaaaaaa tttaaaatta gagcttataa
    52561 atgaatcact atctgttttt atagaagata tgaaagcaat aaatgttagc aaagaagaag
    52621 ttttaaaaat aatagaagat aagtattagt tttttaacaa atagaaaact tagtttagaa
    52681 agtttattta taagcatatt ggatgtggag gatagtaaat atgatagaaa taaagaatgt
    52741 cagcaaaaca tataaaagga tgcaagggct taaaataaga aagatagaag ctttaaaaaa
    52801 tgtaagtttt aatatagaaa agggaaaaat aactgcatta cttggtataa atggtgttgg
    52861 taaatcaact atgttaaaag caatagcagg acttataaac attgatagtg gagaaattcg
    52921 tatagatgga gaaaaaataa atgaaaaagt atacaataaa ttggcttttg ttccagatgt
    52981 acaatctcac tttagcaata caacaataaa agaaacattt gaatttatgg aaatttttta
    53041 ttcaaagtgg aataaagaaa aatcaaaaga aatgatggac atatttaaat tggatgaaga
    53101 tgaaataatt gataacttgt caaaaggaaa cattgcaaga gtaaaattaa tacttggatt
    53161 ttgccaagac ccagagtaca tacttcttga tgaaccattt acaggaattg atttgtttaa
    53221 gagggaagag tttataggag taatagcgca atatatggag gaaaatcaag ccattatcat
    53281 aactactcat gagatagtag agatagaaag tttagttgat gaggttgtaa ttcttgatga
    53341 agggcagatt ataacttcat ttaatgcaga agaattaaga gaaagagaag gtaaatctat
    53401 attagataag atgagggagg tatataagaa tgaataaact tctatatcta ataaatgtag
    53461 atttgagaag aagcaataag ttttatatag cttatatatc atttttttca ttaattacac
    53521 ttagtttaaa tttattcgaa attaataaat ttaaaaattc taaatttatt atgaacttag
    53581 caatagaaga ttttggaggt atattttatg gactagggat acttagtaat cttggtttta
    53641 tccaaagtat attgttcttt gggatattgg gactttttat atactatgtt tttatgtgga
    53701 aaagagagtt cttttcaaat aatcaaagta tttatacact tatgatgtta cctcaaaata
    53761 agtttaagat ttgtatatct aaatcaatag ccttgataac aatgatatat ggttttttga
    53821 taagtcagac agcaacatta tttatatgta aatatatatt taatttcatt tttagaaata
    53881 tgcctataat caatatgaac tttgccaaag atttatatta tttaaacttg aagagtatac
    53941 caattgattt tatctctttt atggctattt atgtattttt actacttata gctatttcta
    54001 ttgtaaattg ctgtatatta tttatctttt cctatataaa taaattttat gtaattttaa
    54061 tcactttg
//
LOCUS       DAMJWU010000012        53554 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMJWU010000012 DAMJWU010000000
VERSION     DAMJWU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.012"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            300..698
                     /locus_tag="PC460_000907"
     CDS             300..698
                     /locus_tag="PC460_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2599804.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(915..1124)
                     /locus_tag="PC460_000908"
     CDS             complement(915..1124)
                     /locus_tag="PC460_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599805.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1156..1365)
                     /locus_tag="PC460_000909"
     CDS             complement(1156..1365)
                     /locus_tag="PC460_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599806.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1664..2083
                     /locus_tag="PC460_000910"
     CDS             1664..2083
                     /locus_tag="PC460_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599807.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            2311..2766
                     /locus_tag="PC460_000911"
     CDS             2311..2766
                     /locus_tag="PC460_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599808.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(2973..3233)
                     /locus_tag="PC460_000912"
     CDS             complement(2973..3233)
                     /locus_tag="PC460_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599809.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(3496..3849)
                     /locus_tag="PC460_000913"
     CDS             complement(3496..3849)
                     /locus_tag="PC460_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599810.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANVEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(3866..9187)
                     /locus_tag="PC460_000914"
     CDS             complement(3866..9187)
                     /locus_tag="PC460_000914"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599811.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNALISQYT
                     EISLSNVTDAYSSYNATFIVVDEKKVYATGINTNYLLGFSTSDGSNVNLGLLSDWYYI
                     NISGSSYSRVSCTNNITKINNIIIYEYVTVFCTNIGSFLTGYHGTSWTKPTDSSYRVQ
                     YQGISYAGYLDSYIYNYYPTRCTQSSSSTTFAYLYNGESSSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNIFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGLVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(9204..10187)
                     /locus_tag="PC460_000915"
     CDS             complement(9204..10187)
                     /locus_tag="PC460_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2599812.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(10188..10886)
                     /locus_tag="PC460_000916"
     CDS             complement(10188..10886)
                     /locus_tag="PC460_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2599813.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(10883..11935)
                     /locus_tag="PC460_000917"
     CDS             complement(10883..11935)
                     /locus_tag="PC460_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2599814.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(11928..12356)
                     /locus_tag="PC460_000918"
     CDS             complement(11928..12356)
                     /locus_tag="PC460_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2599815.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(12356..12682)
                     /locus_tag="PC460_000919"
     CDS             complement(12356..12682)
                     /locus_tag="PC460_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2599816.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(12698..14227)
                     /locus_tag="PC460_000920"
     CDS             complement(12698..14227)
                     /locus_tag="PC460_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2599817.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(14509..14931)
                     /locus_tag="PC460_000921"
     CDS             complement(14509..14931)
                     /locus_tag="PC460_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599818.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(14931..17384)
                     /locus_tag="PC460_000922"
     CDS             complement(14931..17384)
                     /locus_tag="PC460_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599819.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(17384..17551)
                     /locus_tag="PC460_000923"
     CDS             complement(17384..17551)
                     /locus_tag="PC460_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599820.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(17578..18024)
                     /locus_tag="PC460_000924"
     CDS             complement(17578..18024)
                     /locus_tag="PC460_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2599821.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(18138..18566)
                     /locus_tag="PC460_000925"
     CDS             complement(18138..18566)
                     /locus_tag="PC460_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2599822.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(18580..19644)
                     /locus_tag="PC460_000926"
     CDS             complement(18580..19644)
                     /locus_tag="PC460_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2599823.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(19649..20092)
                     /locus_tag="PC460_000927"
     CDS             complement(19649..20092)
                     /locus_tag="PC460_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599824.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(20341..20781)
                     /locus_tag="PC460_000928"
     CDS             complement(20341..20781)
                     /locus_tag="PC460_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599825.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(20886..21083)
                     /locus_tag="PC460_000929"
     CDS             complement(20886..21083)
                     /locus_tag="PC460_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599826.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            21252..21572
                     /locus_tag="PC460_000930"
     CDS             21252..21572
                     /locus_tag="PC460_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2599827.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            21654..22160
                     /locus_tag="PC460_000931"
     CDS             21654..22160
                     /locus_tag="PC460_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599828.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            22861..23616
                     /locus_tag="PC460_000932"
     CDS             22861..23616
                     /locus_tag="PC460_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2599829.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(23759..24187)
                     /locus_tag="PC460_000933"
     CDS             complement(23759..24187)
                     /locus_tag="PC460_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2599830.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(24608..24808)
                     /locus_tag="PC460_000934"
     CDS             complement(24608..24808)
                     /locus_tag="PC460_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2599831.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(24991..26160)
                     /locus_tag="PC460_000935"
     CDS             complement(24991..26160)
                     /locus_tag="PC460_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2599832.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            26396..27217
                     /locus_tag="PC460_000936"
     CDS             26396..27217
                     /locus_tag="PC460_000936"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF2599833.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRHEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(27293..28681)
                     /locus_tag="PC460_000937"
     CDS             complement(27293..28681)
                     /locus_tag="PC460_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF2599834.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGSLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(28816..29574)
                     /locus_tag="PC460_000938"
     CDS             complement(28816..29574)
                     /locus_tag="PC460_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2599835.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFASSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(29576..30292)
                     /locus_tag="PC460_000939"
     CDS             complement(29576..30292)
                     /locus_tag="PC460_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2599836.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(30294..31001)
                     /locus_tag="PC460_000940"
     CDS             complement(30294..31001)
                     /locus_tag="PC460_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2599837.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGQ"
     gene            complement(31117..31653)
                     /locus_tag="PC460_000941"
     CDS             complement(31117..31653)
                     /locus_tag="PC460_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2599838.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(31755..32825)
                     /locus_tag="PC460_000942"
     CDS             complement(31755..32825)
                     /locus_tag="PC460_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2599839.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(32818..33876)
                     /locus_tag="PC460_000943"
     CDS             complement(32818..33876)
                     /locus_tag="PC460_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF2599840.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(33863..34195)
                     /locus_tag="PC460_000944"
     CDS             complement(33863..34195)
                     /locus_tag="PC460_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2599841.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            34394..35515
                     /locus_tag="PC460_000945"
     CDS             34394..35515
                     /locus_tag="PC460_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF2599842.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(35643..37541)
                     /locus_tag="PC460_000946"
     CDS             complement(35643..37541)
                     /locus_tag="PC460_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF2599843.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            37856..38632
                     /locus_tag="PC460_000947"
     CDS             37856..38632
                     /locus_tag="PC460_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2599844.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(38724..39476)
                     /gene="xth"
                     /locus_tag="PC460_000948"
     CDS             complement(38724..39476)
                     /gene="xth"
                     /locus_tag="PC460_000948"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF2599845.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(39637..40587)
                     /gene="galT"
                     /locus_tag="PC460_000949"
     CDS             complement(39637..40587)
                     /gene="galT"
                     /locus_tag="PC460_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF2599846.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            40972..42168
                     /locus_tag="PC460_000950"
     CDS             40972..42168
                     /locus_tag="PC460_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2599847.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(42280..43620)
                     /locus_tag="PC460_000951"
     CDS             complement(42280..43620)
                     /locus_tag="PC460_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2599848.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     HQIAIVITENNGAISNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(43759..44511)
                     /locus_tag="PC460_000952"
     CDS             complement(43759..44511)
                     /locus_tag="PC460_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2599849.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(44659..46236)
                     /locus_tag="PC460_000953"
     CDS             complement(44659..46236)
                     /locus_tag="PC460_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2599850.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     MPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGIIAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYAIVYYNVFKILILRFNLQTPGREEITDINFDLKPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(46648..47100)
                     /locus_tag="PC460_000954"
     CDS             complement(46648..47100)
                     /locus_tag="PC460_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2599851.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(47411..48856)
                     /locus_tag="PC460_000955"
     CDS             complement(47411..48856)
                     /locus_tag="PC460_000955"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF2599852.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(49000..49908)
                     /locus_tag="PC460_000956"
     CDS             complement(49000..49908)
                     /locus_tag="PC460_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2599853.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            50165..50989
                     /locus_tag="PC460_000957"
     CDS             50165..50989
                     /locus_tag="PC460_000957"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2599854.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            51029..51559
                     /gene="lepB"
                     /locus_tag="PC460_000958"
     CDS             51029..51559
                     /gene="lepB"
                     /locus_tag="PC460_000958"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2599855.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(51641..53422)
                     /locus_tag="PC460_000959"
     CDS             complement(51641..53422)
                     /locus_tag="PC460_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2599856.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT        17625 a         8777 c         5931 g        21221 t
ORIGIN      
        1 ttaatctttt tctcttttac agacaatcaa aaagcaatct tagtttatcg aaataaattt
       61 atacaatcta ttcatacacc cctttacatt tatataacaa ttaaacctat tgttatatat
      121 acaaatcaaa ataaaccata tattttcaac tataatatgt taaaatcata ttatgagcat
      181 attaatttaa gcaaaatgtc atttagactt taatttagta gttttatatt ataaatttat
      241 atatttttaa ttttaaatgt catttagaca ttactcataa aaagaaagga gtgtattttg
      301 tggatacttt aggaaaaaga attgcttatt tgagaaactc caaaaaactt actcagcgca
      361 aacttatgga tattttaaaa tttgaaaact taggcaagta tgaaacagga gatagaaaac
      421 ctaattgtga tatactaatg tcaatagctg attattttaa tgttacgact gactggcttc
      481 tctatggtaa agagaaggta aatgtcaata gtagtgtaaa agaagataaa gaagattact
      541 tacatgttac taatgatgaa atgacgatat taaatctata cagacaacta aacgaacgtg
      601 ataaaataaa aatagaagga atacttgaac ttaagatatc tgaatataaa gacttaaaaa
      661 agcattcctc taataataat gaagataaaa tggtttaaac atacataatt tgatacgatt
      721 ttacatcatc tattaacctt ctttttttgt agtatattac taaaatatgg atattggatt
      781 atttaaaaac aagtatagtc taatttcaag cacatttata atatttagat tatcttatcc
      841 atattttagt ttaagtactt tgaaaaatat aatacatctc tatgtataac ttatattcct
      901 aagaaatgca ttactcatgt ttttaattct tccaacatct ttctatacac aattgtacat
      961 ccttcataat tcccctcttt aagaaggatt gaacatgtac tagaatattt agttaaagta
     1021 tttaatcttt ccatatttaa gctttttata ttactgagtg ctgtaataaa atcatcaatt
     1081 gctctacaac actctataac cccatctgaa tccaatttac acacaagaat attcacctac
     1141 tttatatact atttattaat catcttctga atattctata ttaagtaatt gataaattga
     1201 tgatttatat tttttacctg ttcttcttcc atttaaaata gctgacaaat agttttcatt
     1261 aacacctatt ttcttcgcta attccctttg agtcatattt aattcaataa gtctttgctt
     1321 gacttgcact cccaaaaaag gtatccattt ttttcgagca tccatttatc aacttccttt
     1381 ctaagcgatt tatatttact tttatattat tttagcttac gcaccccctc tatttagtat
     1441 atatttttaa tatgtacaca tattaagtat ataaatttat ttaatactaa ataagatttt
     1501 ttgattactt tgtgctaaaa ttaggatgta aattcgtatc attaagttaa ttataaagtc
     1561 attttgacct taagtcaatg gtttttatgt cattttgacc tttaatttga aactatttac
     1621 atcttaaaaa gtctttttga cattaattga atggagagtg tttatggata ctttaggaga
     1681 aagaattgtt tatttgagaa aagcaaaaaa ccttaaacaa tatgaactag aagaaatgtt
     1741 gggctgtgac aacttaagta aatttgagag aaacattaga aaaccaaatt atgaaatatt
     1801 aaaatctata gctgagatat ttaatgtttc agtagactgg ctattaaatg gagataatct
     1861 atcacataaa tcagatttaa tttgcgattc ctcctcaaac tatccactta atagcataaa
     1921 ctctaatgaa ataaagcttt taaacaattt tagaaaatta aatgattatg ataaagctaa
     1981 aatagaaggt atgattgaat taaaacttca tgaatatgaa aaagagaagg atttaggaaa
     2041 aattgagtat aacaaaaata aagatgagaa aatagataaa taatttttat gttgcttttg
     2101 atacgatttt acatcatttc tttgttctaa tatttttatt tcatttattt tcttttttat
     2161 aattttatga taaaataatt agtatatttt gctttacttg aatgtaatta tttatacaaa
     2221 agtcaaacat aatataaatg tagtaaattt ttagtttcaa gtaatatctc gttattataa
     2281 ctaaatattt attaaaatta ggaggataaa atgggcttaa gagacaaatt cgcgcaatca
     2341 tttgctagat caaaaactat gagtggccct gagaaaaagg ctaatgaaat aatgggtaaa
     2401 ttgcttctta agaaagctat attacctatc gttttaatgt tcgtgataat aatcgctgga
     2461 gctatgctta aaatcaattc ttgggttaca cttggcataa atttagttat tgctgttggt
     2521 gctttcttct acataagaaa ttctagtaaa aagtatcaga actttaaacc ttatgttggc
     2581 aatttgatta gtttagagaa aaaaggtaaa aaagagtacg ttgctattat aaaacaaggt
     2641 aagttaccag ttaagttaca aatagcttat ggtggtgaag accttgaaca tgttaagaaa
     2701 aatcaaatgg ttcaaataag ttataatcca gatgctaaaa tagctatttt ggttaataga
     2761 caataattta ataaaaaaaa ggagttgtct taaaattgat tttttctaat caaatttaag
     2821 ctcaactcct tttttaattt ttatatgtta tttttattac tttttttaat taatatttta
     2881 aatattaaat ccttctcaat aaaatagtga actatctaca atactacttt atcttttcct
     2941 aacaatatat aatctggtgc tactctttca tgttatacac cattttgtaa catacctttt
     3001 atatcagtaa catcttcctt aatttcctct acatctgttt tcattatacc catttgaaca
     3061 agtatatttt tgtttatttc ttgttgttgt gtagataatt ctataaaatt ttctacagtt
     3121 tttttataca tgtctctatc ttcttttttc tcctgcatag tatttttaaa taatagagca
     3181 catagtatac ctattgcccc tagactactc aattcggtta ttaatttatc cattatagcc
     3241 tcctattgtc taaatttttt atacttatag ctttaatttt tgttgtattc ttacatcatg
     3301 caatattata ttttcaggat tatcaattta atcttctgta ctttaaattg taggtgtaat
     3361 ttatagattg aataaataat aaatatatat acaagttcta atgtaacaaa aaataagtac
     3421 aaggcttact tattaaagtt aagaccttgt actttacata aaaaaaataa ctaatacata
     3481 gttattctaa atttactatt caactggtat ttctaattct ttacatctaa ttctaacttg
     3541 ctctctaatt actgctggta cttcttctat tgtttttttg cctcttaaaa ttaaagctac
     3601 atatacttct accattccac tctcacctcc agattttctt acactaaaag tgcttatatt
     3661 acttgcttca ttgacagaac tttgatttaa caacatctca tatatttcta catttgctat
     3721 aaaattattt gttatatcat catcttgctt tacttgacct atacttatgt ctgaaatgtc
     3781 tttatactgc atttctactt ttctaccttg tttgatttgt tctattttat ttttatcgaa
     3841 tttaaacaat tttcacacct ccattttaag cttgtggctc gttgttttca gcatcatatc
     3901 taaaaatcat agatggacgt tttatagctg tccatttatc tcctttttct actatgattt
     3961 taggctgagc actttttatg ttggcttctt ctaattcata gatttcttgg taacttgagt
     4021 ctgatttttt gatttcaact agtttcatgt ctttgaataa gtcagattta tttattttta
     4081 cttgaacttt agttttattt attaattgat atataggatt gttttccatc atatcatcct
     4141 ttaatccctc atttaatgtt actattaagt cagtgttatt ttcagtaatg tttatgattg
     4201 aatatacttt attgtttatc attaaaatag aatccttttt tactttaagc cctgtttctg
     4261 atttaaatat aaacatactt gaatataaat cgcctgtagg actactggct ttaatcagta
     4321 tcttattttc tcctaatact attttatttt gtggtatctc aaatataatg ctatttattt
     4381 ccaaattagt cttagtagat attaattcat catttataaa catttcaagt ttttctataa
     4441 cattattagg gtcttgtact acaactctca cttgagatat tgatttttca actatttcta
     4501 tagatgcttt acttgtatct acttttgtta tcatgtatat tgtatcatct gaaagctcta
     4561 tatctaaaat atcatttaga tgttcaggaa ttggtatttt agtaaattca tataaatcag
     4621 taattgatat agatatttta tacatagatt tatttttacc agttatccat agactattat
     4681 catttttaat tactagtatg tggtcatcat tatgtgactt aatttcttta acattagtta
     4741 atactttagt aaaagttctt attatcttat ctttttcact ttctcctagt ccaaataatc
     4801 catttgtatt gtacccttga gcgtacaact ctccctcatt agttaaaaca agtgtcattg
     4861 agttatatat acttatatct ttaacagaag gtaatccagt aaccttttgt aactctctta
     4921 catcattatt gtggccaact cccaattggt actcagtatt ctttccatat gaccatagag
     4981 tattatctgt cttaataata taggagtagt tatctccaca agcaactttt ttgacatctg
     5041 atatatttac ttcagtaaga gttaataatt ctatatcttc aagggtttga ttaatttctt
     5101 tatatgaatt acttcctatt ccatataact tgccatcctt agcaacagcc aatgaatgtg
     5161 tttttgatat atcaatatca gatacctcaa atggcaatat tattgaagta tacttaaaat
     5221 tatctttaaa tatgttttta taatttatat acttattatt tgtttttata tacttatcat
     5281 taaacattac tagcttttca tttttcttat taaaaactat tttatctgat gtgttatctg
     5341 ttttaaaata tgaatgtagg gaaccttcaa gagcttcatt tttatcatta tcatatctat
     5401 ggtgaatacc taaatgatta tatctattat ctcctgttga agaataaaat tttccatata
     5461 tatctttaat aaatatagta ttgtaactag aaaatacagt atcaacatct ttctttatag
     5521 atactttata atatggaact gacatatccc tataagattc atcagatata ccataagtat
     5581 ctctacctga tgatatcaaa ttgttattat tatctatata aagtgtatta ttagctgtta
     5641 caaaaacatc tttaacatct tccacactat ttgtattatt attatttgct atacttccat
     5701 atccactaag acctccaaca aatagtttat tgtcatttgt taaaatggct gaagtatatc
     5761 cattaactac tatttttttt gcatttataa tattattaat tactcgtatc ccctttatat
     5821 cattagtctc tgttgaaaag aataccctct cttttttccc tgtagtataa atcatattgt
     5881 catttgttat aaaaaatgta gcttcacttc ctgctgtcac aacagatttt acattctcaa
     5941 tatttatttt tgtaaatgta cttatacttc cttcagtaaa cccaaaatag ccatttacaa
     6001 gtccacatac atagcatgta ttatcatttt taacaatgat tgatacatca ttacatagat
     6061 atacatctct aacaccattt ataggtatac gtgtaaatat attcctactt gtagtatcac
     6121 ccaatcctaa ctgtcctttt ccattattac ctgctccata taaaagacca tcattagtta
     6181 ataatagaga tgtgtttcca tatacagcta catattttac attttcaaca tttattttag
     6241 taaatatatt tctatttaca ttatctccta ttcctaactg accagaatta tttaaaccaa
     6301 cagcataaca ggtattatca ttttttatcg caaatgcatg tttactattg tcagtaaatc
     6361 tatttacatt tatagatttt atattatcaa tatttatttt agtaaatacc tttctattta
     6421 tctcatctcc cactcctaat tgacctacat catttaaacc aacactgtaa agcatattat
     6481 tattagttaa taaatatgta gactttgaat ggctatatat ttcttgtata ttagaagata
     6541 tttcactact gctattcttt ttattgtaat ttatcttatt atttaaattt aaactactat
     6601 taactgatat accatgcgta cccttagagt tatatccaca tataaataag ctcttatcag
     6661 ttaatattgc aaatctagtt ttatctctac ttaaatcata aggaggtata aatatatttt
     6721 ttatattttt cattaccttt ttataattag tggcattata tccatcttgt gtatctgaaa
     6781 catctagttc ttttgcactt tcagatatat tcccaaaacc atatagcgtg ttagaagata
     6841 aatacaatag agctttatat aaaaatatgg ctttatcaga ggtaatagga cctgaattta
     6901 tattagatgc tgcaatatta ttagatgatt gattgttaag ataattcctt ccatattgat
     6961 gtatataaga tgaacctcca gaaataagta aattatctgg attgacattt tttaaattac
     7021 ttgacgattc cccattatat aaataagcaa aagttgtaga agatgatgat tgtgtacatc
     7081 ttgtaggata ataattatat atataagaat caagatatcc tgcatatgaa attccctgat
     7141 attgaactct atagcttgaa tcagttggtt ttgtccatga agtaccatgg tatccagtta
     7201 gaaaagatcc aatgtttgta caaaatacag ttacatactc atatatgata atattattaa
     7261 ttttagtaat attattcgtg catgaaactc tactataact tgaccctgat atatttatat
     7321 aataccaatc acttaataaa cctagattta cattagatcc atcagaagta ctaaaaccta
     7381 acaggtaatt tgtatttata ccagttgcat ataccttttt ttcatcaact acaataaatg
     7441 ttgcattata tgaactataa gcatcagtta cattactaag gctaatctca gtatattgac
     7501 ttattaatgc attactaagt ccattaacat aacttgaact ctttccagaa gtgtatattt
     7561 gcttattatt agttacaatt aatgaaaacc agtttgataa atcagtattt ataacaactt
     7621 tttttacatt atctatagat attttcttaa atacatctga attagaagga agaacacctg
     7681 ctattgagtt actatttggt ccagaataat aacaagtatc atcattttta atgtagaata
     7741 ttcctccaga aatatttttt gatgctacta tagactttac attagagata cttaattttg
     7801 tcaactcatt taattgactt ggtaagtcag aacccattcc tttgtatcta ttatttccta
     7861 gaccatatac atctccattc cctaaaataa ccagagtaca ataaggacta aaaacgacat
     7921 ctcttacaga atgtatatct agtggaagtt caatatcctt tatttccatt tcttctttta
     7981 ataaagttaa tttatcctca gatttaatta aatttatata attactataa ctatattttt
     8041 cactatccat tttcttaaat tctacaatgt gcatatcatc aaaaaacgaa cctaattgtc
     8101 cataagtatt atctcctgta caatatgaat cattatttga atcaattagt atgctatgtg
     8161 ataaaccaat gaaaatattc ttagctacaa tattttttaa atctataaat gaattatcta
     8221 tattctcaga gtaatttgct atacctaact gatatatatc attatttcca gcaaccttta
     8281 aagtaccttt gttatctata agcatagtgt taccatgacc acatgcaata gtttttatat
     8341 ttacttcttc tatcttagta aatagtgatg attgaagtgc atttccaaaa catttttcct
     8401 ttccacaacc atatatatca ttattatacg tcattaacac agtgtgttca gaaccacaag
     8461 ctatagtctt aacatcatta atagaaacta aactaaataa atctctatta ttcttatctc
     8521 ctaaacctag ttgaccttga ttattaaaac cagtagaata gcatttattg tcattcttta
     8581 ttataaaagc tgatttatta tatgaaaata cagcctttac attgtcaaca tctacttttg
     8641 taaatgtatt tacatttgca gatgctccat tagttcctag acctaattga ccatacaaat
     8701 tatttccagc aacatataaa gttccatcat tgtataaaag atatgttgat tgaagtccac
     8761 atgcaatatc ctttacgcct tttatttctg gaaccttctc aaatgtagaa actgttttcc
     8821 ctactttttc taatcccaat cccatttgac catctgtatt tatacctgcg acaagtaatt
     8881 ctccatctga agtaagtata actgcatgag attctccaca tgcaactttt ttaatattgg
     8941 tagatatatt tggtattttc gtaaataatt ttcttgttgt attgtcttct cccaatccaa
     9001 gttgaaagtt actattatct ccacatccat acaagatatt attatttttt agaccaaatg
     9061 tatagttaca acatccagta aaaaaattaa taaagtcata attatcatac ccttcatcaa
     9121 attcatcaac taccatattt ttatcgccaa agaaattacc tctttgaagt agtttagttc
     9181 ttttcattat aaaaacctcc taattatctt atttcatacg aatatttatt taaagttata
     9241 tctgtctgac caattgaata tgccctatta gattttgttt caacaaagtt attatccatt
     9301 tcatgtatat atctaactct atttgcatta aatggtagca ccacattctt aaatgtaaca
     9361 ctaccaagtt ctccagttat acactttcta tttaaatcta acttaaggcc ttctatagct
     9421 attatgtttt tagcattagt taaggtatca taccaatatc cagcttcatc tacatctatt
     9481 tctgactcta attctaagta tattaagata tctaatactt tttcagataa tttattgtaa
     9541 ctatttttta atacagatag ttcagtttgt atttgacctc ctattacatc atcaatagat
     9601 atatttttaa ttttttctac tacctcattt atagctcctg ataagttttt agcttctgta
     9661 gataagagtt cttcgccaat ttttactttt atttctttta tatttttatc aaacttatca
     9721 tctaaatctt gaatatcctt ttgagttaca aaaaccaacg tcgggtctac ttctaatgta
     9781 acatttgaag tattggacaa ttgtaaaatc atttttacaa ccaaatcttt aatactgcca
     9841 tcttcagcac gtggcttata cgtctctgga tacttaccta tagctattat attattctca
     9901 ttatccagaa cagcagcttc tcttatcata aatccaccaa ctgttcctgg taaaatagtc
     9961 tctataacta tccaattagg atttttttca tcaattctta gagaatttat atttccttcc
    10021 caaactacat tctttaaagc agtttgttcc tctgttggct cataatattc tccattacta
    10081 tctccaactt gaaacttggt taatccaaca agctctccta gtgcagttgc atttgctatt
    10141 ttttctctac caattttggt taatattata taaaattttt cttccaatta taccacctcc
    10201 gatttaggat aaactactac attttctaca cctgtatcat ttgcaatagc tatattaatc
    10261 ttacaattat tttcaatgca tttaatgctt aaaggataaa cctttatttc ttctctactt
    10321 atcattccag ctccacagtt tacaacagca ctacatttta taattggttc aaagtatgga
    10381 tgaactttta cctcttcact acataccata cctccaccac aatagaccat acttttatct
    10441 agtattattg gttctaaata gtgtaataag tgagcaggtt ttactctttc aatcatatcg
    10501 ctacaatcta aaagagtttt atagtcacag ttatttgcta taaaactcaa tacgaatgta
    10561 aattcatcac tataaacttt tatatctgtt tctgattttg tatatgcctc acatatactt
    10621 tttataactt caatagtact agtacctctg cttctcattt tagcttttat attgctacgt
    10681 cttgtgtcaa aatcaagttc ttttttttca atgcagagta tgtcttccca catatctaat
    10741 ccccatgttg cagtgtcaac aaataattgg ttaaaggtat catcaatatc ttctttaaga
    10801 atatttaatt ctgtatcgta tgcaccttgt atctcctcta caatgtaatt tctatcaaat
    10861 gatggtagtt tatcaattag cttcattgat tttccacctc actaaaatta acagttgtta
    10921 cactaggtat tttatcttca ttaatagtta tattatctgt acttccattt ataagtaggt
    10981 tacttaaatc gtgtacacca gtagtattta taagtattcc cattactttt gtatagatta
    11041 tctctcctct aatatctcta aagtatgtat ttatactttc taggaaagat tcttttacat
    11101 tgtctaatgt atatccatct tctagtttca ttactgcact tatacttatt tctattggta
    11161 atggtgtcac aactgttata gttggtccaa taggcttctc ttcatctata tgttgctgac
    11221 acctttcaat tgtttctgta tcaacagctt gattattttt cccaaatatc aagaccttaa
    11281 ctgttcccgg accatcccat cttggataaa ccttaacatt atagactcca tctacttcca
    11341 aagcccattc ttcatagtga gctttatttc cacttgtagc ttgatttctt tggattttat
    11401 aaaatctttc ttttagttct tcatctgtct ctatatctgt accaccttca aaacttcttg
    11461 tgttagtaat ctttgttact ccagatatat tatcaactag tttaaattca caatttgtag
    11521 gtaaattata tttcttacca acttccagag cttgaactgg gctattgtca ccttcttcac
    11581 taccaatagt tacatctttt attactacaa atagtaaatc tctatatgat attattgtgc
    11641 cattatttat tacagtacct ttctctccaa taaattcaac ttctccattt gactctgtac
    11701 ctaactttct atatacacca aattcattga ctcttttatc aagaaactgg ttatatgtgt
    11761 cttgtataaa agccatttta tgcatctttg aaagttctag atatatcttc gaaagttcta
    11821 gattatttcc agataccatg ttgtttagaa aagaaccttc tcctttataa atatcaagat
    11881 taatattttc aagagttcta ttttttatta cttcatatgt ctggtcacta tacatttatt
    11941 tccacctccc catagatagt ggatactttt acatttgcac tcaatatgtc tctgttaaag
    12001 ttagcacttt ttacgtttac ttctaatata tatggattta ttagaagggc ctcttttatg
    12061 aatctactag cttcactttc tgtaagacct tttgtatatt tttgtcctat tagttctgaa
    12121 agctctgtcc catattctaa agagtatatc tcatgttcat atctatttgt ctttatacac
    12181 ttgtatatcc aaacttttaa ggcttctttt ttttcaatta ttttaaagtc acctttttct
    12241 aaaataggtt catctttttc aaaatcccat gccacttcac gaaagattgg caattcttct
    12301 gttttaggta agatataatc ctctgggaca cctataaaag gaaatattgt actcattata
    12361 agctcaccaa cttacttaca acagcaaatt tttcgcctat tctaaacatt attaccatat
    12421 ccccagattc aaagttatct ataaaaggat tttttacttc atgctggtgt tcttgatttg
    12481 tttctgtgtc aaatgtttct agctgtctat caatcatcca actatctatc aaaatatctt
    12541 ctttttctaa tatgatgtta tttgtctcta tttttaaatc tggtaattta cttttaattt
    12601 ttccaataaa aaaagaaggt tgattatgat actttccttc ttctcttatt attcctataa
    12661 attcatttat tggattagcc attatatcac catcctttta taatactctc ctagctgtgt
    12721 taaaatctct tctactactt aacttactta ttttaactac atcacctttt tgtggagcat
    12781 gtaaaaattc tccattacct atatataatc caacatggct gactggatta tgaaaaaata
    12841 ctaaatctcc tgcttgaaga tttttttgtt ctactttctt gcctttttta gattgttgat
    12901 ttgacgttct tggcaaacta acattaactt ttttaaaaca atagaccatt aaaccagaac
    12961 agtcaaaact acttggtcca tttccacccc atttataagg tttacctaga tgtttttttg
    13021 cttctgatat tactatttta gctttatcag atacattacc agaataacca ccaataataa
    13081 tttttccttt tcttcttcca aatttactag cttcttcaac atttccaaat aaaatatcta
    13141 tatggtatgt tccattcttt tctatagtta ttgcaggtcc tacgtcattt actttataga
    13201 ctccatcacg accagaaact cctgtaatta cctgtatttc atctccatag tttaataaag
    13261 gatgtttatt taaaaactct tttgtataat aagtttgctc atatttacca accataggag
    13321 cagcgcaagt ttttttagat gggtcaagtt ttttctttct acaatctgta tctccacctt
    13381 cttcttttct aggacagtaa gctgtaaatt ctgctgtaaa ctcttttcct cctgcataat
    13441 cttctccccc taaattactt tcttccttag gttcatcctg tcctgctgac ttttcatcca
    13501 taagattttg aaaattaagt tcaagctcaa tttgatattc tccattttgc caagtatgtt
    13561 tgtctgtatc tatataaaat agtcctacaa gctttgtata agaatcttta acttttactc
    13621 ctctaccagt tatacaactt acatctccat aacctttaag agaacagctt ttttctatcc
    13681 cattaaactc gctatcaata tctacatctt gattttcttg ttgctgaatt actttttgca
    13741 ttattacatt tacttcctta aaaatttcat tatctatttt ttcgctaatc ttgcttccat
    13801 actggtctac cactattact ttatttttta cattttccat gctctccgaa aaggtggtat
    13861 ttataatatt aaatccctct tcaaacataa cacttaatgt aacagttcct ttttcaataa
    13921 cattaaactt atctaaattg gcctctatca tatacttttt ctttgtcttt ttacttgctt
    13981 ctgtatatgc actcattatt gtgtcataac catttactcc tataaacatc tttgtatact
    14041 tgacattggt ctttgctatt attccaactg aaagcctatt ttcagcaaat acttgctttg
    14101 ctatgtcttc aactaactta tctttaaaat tgtatgacac ttcactttgt gtaagtaaaa
    14161 atcccatatc tttagataca aaactaattt cattactgct tgaatcttta gacctattaa
    14221 ttatcattcc tctaaagagt tctttctcat ctacatagaa acagactgta ctagctattg
    14281 gtatatcgat ttgttggaaa tttacatcaa atgatgattg tattattgaa aactctagtg
    14341 tccttgatgg agatttataa tcacctgacc atgatacctt gtctactatg tcagttatgt
    14401 catatatact tccgtttttt atgtgtaccc aaatcttaat aataaaaaat cacctgcctt
    14461 ttcatttaat tctatcagca gttatttata acttattatc tataatagtt attgttttgg
    14521 agttactttt ggtatcttta ttcttttata ctcttttaaa tctaatgtaa aatatacatc
    14581 tcgagtccca tctctttctt cataactaaa cccttcaatt atgacttcca tatttatatt
    14641 tgtttccgta attgtaaatc ttaatataaa accttccttc atccattttt ctatcttatt
    14701 tacacaatca tatggtgatg gaaaacctgt atagtcacag aaagtatagt ctttatttgg
    14761 aaaaaaactt gatattgaaa tgcttttaag agctacacca ccaaaagttg ctatttcacc
    14821 tattttgagt atactagaag agtttacagc agcttttcca ttaatactaa aggaagatgg
    14881 aaatactgga aatctaaatc tatcttcagc ttgtctaagc cacatttcca ttataagaca
    14941 ccccctaact gtgccaattg gatacttgca actaattttg atgttatctt ttctatatca
    15001 gcttcttctc taatcactat tgtatcagct aacttagcta tgtttatatt tccaccattt
    15061 tttctgcttc tatattgatt tgcttcttgt ttagttaaaa ccatttcacc ttcatggagt
    15121 ctggtttgat agtcattata aggaacatag ttcaatccaa atgcattatt acctttctta
    15181 cctgaataag ctttaccaga atcaccagcc tttgttccag catatggatt atctttgtaa
    15241 ttttttttac tcttttccac tagatttaaa actgttcctg tgatgcttcc attttttatc
    15301 ttattaatca tagtttgcca ccatttacat atatctttaa ctattttaca tactttatct
    15361 aatatgttaa aaaaagctcc caatattggc tctataattt tccatccagt ttccaacaga
    15421 ggtcctaagg tctcccatac agttttccaa ataacaccca gtgtctctat aaaattgttt
    15481 atttgaggtg catattcacc taaaaaagta aaaattcttg tgataatagg actaacagct
    15541 tcaactacag gtccgattag ttggaatgct gtagttacta ttgtaccaaa tatacttatt
    15601 aatccttgtg caattggtga tttattgttt atactatcta gtaaattatt tactaagttt
    15661 actactggaa gtaatgaatc tattaatgct gtaaaatcga tattttgtaa tagtgtactt
    15721 cctgcgttaa caattgcttt tggtgcattc cctatcattg ttgctgcatg attttcaaaa
    15781 cgttcttgct tattttcaac catcatttta cgcttttcag cctcattaga aattcgatat
    15841 tgagtagaat cagaaaactc ttttccattt tttacacttc tagattgcat ctggtcatat
    15901 ttgttgccaa taattatctt accattcatt acattgccta ttttaccagc cattttttca
    15961 gataagccag ctaaattgcc taatccactt tctataaaag tttttatttt ctttagtcct
    16021 tgacctaaaa tatcatcaaa aggtttaaca atactcttca agccattgtt aatgcttgtt
    16081 tgagctcctg atattatacc tggtattgta ttactcattt ctttagctaa ccccatctgc
    16141 tttacactta tgccttccaa tgcttcatca aaagttttat aattttctcc taccagagta
    16201 ttaagagatt ccatgtttcc ttttgctgca cttaagaaag ctgctgatac atcttgttca
    16261 ctacttgtat tcatatttaa agctctaaca tctaccatag cttgagttat atctttttct
    16321 tttttgccac cagtaatatt actaatcatt ttagctttag ttcccattgc aacaacatct
    16381 tcagggtcaa aaggcgtatc atttgccatt cttactaaac tgccataata atcatcaaca
    16441 tctatttgat taacttttcc agtatttctt ttcattacac tttctagaac aactttctga
    16501 ttttgttcct gaccaagtaa atcaacaccc tttttcacag cgacacctat agtagcacca
    16561 tttgtcaaat cagatatctt cccttttatc gaaccaagta ttccacttgc catatccttt
    16621 acagctattg caccactcca taccttacct gcaaataatc tcaatttggg aaatacttta
    16681 cttataatgc cacttgccat atcttttata gctattacac cactccaaaa agtattttta
    16741 aacaacatta acttaggcaa aattctaccc atagcactac tcaccatatc tttaagatta
    16801 atacttcctt gataaggacg tttctctact tgttttaatt ttcccaagat actacttatc
    16861 actctcttag tcatatcttt tgcttttatt acgatattcc aaatcttgcc accaatcttt
    16921 tttaaatttc caagtaattt atttttagtt ttttctactt catcactttt agctttaaat
    16981 attaatataa tcataatatt tgaattaaac tcatttttct tttgaatcaa cttattaaaa
    17041 taatcaataa tcatagctat agttattgct agtctcataa tacttccatt taaacttcca
    17101 ataactcctg aaacagcatt tatgctagaa cttaattttg tcatagttga acttacacta
    17161 ctagcagtag tttgaacttt tcttaacgag gaatttaaac tgtttactct attacttgca
    17221 cttccaatag aattttttat gctactagcc attacattag tggaaagtga aacagccaca
    17281 gttgctctcc taaaattatc agttactgat ataatttgat tcatttttgg tgtgtaatta
    17341 tctgtaaggt acatatttat accttcttct ctcacatttc ccattacata ccacctcctg
    17401 tcaaaggaca cactccatat ccatgtttca ttttttcaac ttcttcctca atttctaagg
    17461 cgaaaaaagc ttcaataagc tttaattcgc ctttatccat tgcataaaaa agggacggtc
    17521 ttataccttt tttcttccaa taatagtaca ttattgtagt cctggtatcc gtccctatta
    17581 gttttttacc tcttctatca ctgcattttt accataaccc ataacatctt gaagtattct
    17641 atataagttg gtaatttcac ctggtaataa taattttctt gctaattctt ttggtgttgg
    17701 agccttaaat ttatccatta gctccttatt tttaaataaa agctttccat ccaaatcaca
    17761 aacaccttct ataattgtaa gcatctgcat ctccggtaag tctatatccg ttttcatatt
    17821 ttcatcaatt ttaagacaag tattttgtat ttcttcatct ttacttattg ttaaagctct
    17881 acacattact ttaaattcct gcccaaatat agttgataat ctcttaagtt caactatagt
    17941 acttggtctt tctaagtttt ctgtatctga atttaaaagt aaatctacta tgtttatatt
    18001 ttttgataat ccattttcac tcattattat ttgctccttt atcaatcaaa ttcaatcaac
    18061 taactttttt acttattaac tatctgaatt tattaagtat atacttacta aatatcagta
    18121 taaaactaaa tataaaacta aattatatca agaaaatcat actcagtaaa tgtgaaagga
    18181 gcttctatct ctccttttac tccaacctcc caatcagcta aagttaaatc atcaaatgct
    18241 atatttttta ctgctattct ttctgctcca taagaatctg ggtcatttaa ttttgatata
    18301 gcaacaaatc taggttcaga acctctcttt atcttttctc caattaactt gtgcattctt
    18361 gaactaacgt ggtatagagt tattgagcct tttcctttat atcccatata ctttgtatca
    18421 gtacccattt gacctgctat tataatatct tcttttgtaa attccatctt tgcttgaaat
    18481 ttctttactt ctgctacttt gtttccatca agccaaagtt ctccccaagt accactcatt
    18541 acatttctag cttccatatt tgccatatta ataatcctcc tatatttcta ttgataaatc
    18601 tatatcttcc atagcatcaa gtacttttat ttttgctttt aaaaatactt tagaacctgt
    18661 gttagcttct tttatttctt gtaatgtcat ataagataaa tctactcctt ttgattttaa
    18721 atacgatttt tgtgcttcaa aatctatttc aacagtagaa tcagattcta taagtgctga
    18781 tttttctaat tcttctaaat aactttttat agctactatc aataaacatt tgttgtcata
    18841 actgttagta acctttccta tatagtcatc tattatcacc tttcttatgt cactatgtat
    18901 aatatctaaa gtgtcaacta tttttatttt ctggaacatt tctccttttt ctgctgttaa
    18961 ttcagttaaa gaatttactc ctctagcaat tcttatagct cctgcttctt taataagtat
    19021 aagctctcct ttattaaccc ttgattctgc atcaactttc gtcatcttag gtatatcgac
    19081 tacatcacta agtttagtat aagttactga ttgacttaaa ggtgtacctg ctataagtcc
    19141 agccaccctg cttgtaaact catcaacact gtatttcttt tctccaacta acacatcttc
    19201 tgtagtaaaa ttaattatcc cttcatgatt tcctacaact tttcctaata cagctttaac
    19261 cttaacctta tctatatctc taagtttaat tatccaattt tttatagcag tcttatcagc
    19321 ttctactgct tttggcatac atagataatt aaattcctta gtctctaaaa aatctaatgc
    19381 agtttgaata tctgcttctc cttctattac ataaactaat aatttatttg gagtgttaac
    19441 atttcccatc aaagctaaat ttatatattc tttattttca gcagataaat caactggtat
    19501 atcctctttt tcatgtattt cattaagacc tagtgcctta gcatctttaa gtaccattgc
    19561 aattattcct ctagctgaac gttctttaac agttgtagct agctccttaa atgatatgtt
    19621 gatacttggt aatcctatag ccatactatc actctcctaa atttaaattg atttctttca
    19681 tattttcata tactacatct tcttcatata cactatccag atagtttagt gtaaatgtaa
    19741 agttcaatac ataaattgac tcttcttttt taatactttg ttctagcttt gttatattta
    19801 attttctgtc tgttaccttt atatttctgt taaatagctt atttaattca tctgaaattt
    19861 catacattct aatactcttt gactgtggtt ttggtaagta ttttatagag ataacaatat
    19921 ctttgttatt cgtatttaac attacaggtg tacaaataac tgatagtata cttacaaaaa
    19981 aacaagaccc ttcagagcct tgcacacttt catcatcaat aaaaatactt tcagtaaagt
    20041 tttttttgag aatctcaata attgtttcta atatttcctt gtatttaagc aaataatcac
    20101 cacactatca ctaaatattc ttgcttaatc tgataatctt actataaatg atataagtca
    20161 tttttaaata tatatctcat ttattcctgt caagtatctt ctatattttt aggtaatatt
    20221 attcttttca atctatcacc tccttcaata tggatttctt tcacaataac attatctcac
    20281 atattttatt gcctattgtt gcaatggtgt atataaagtg ttgtaaaatt gtcctatttt
    20341 ttataatatc tcactcaatt taaatatcat ctttttcttt aaattgtagc aagatgttct
    20401 atctaagtgt agtttcatag aaatatatgt catattgttt tttgtcttac tattataaaa
    20461 aagattaaaa aattccattt ctatatcatt tagacacgtt agagcatttt ctatcttctc
    20521 tttttctatt tctatatctt ctttttccat ttttaatttt cttaatcttt cctctctttt
    20581 taacacctca ttttccacag aagagcttat gttatatgtg ttactagtct tttctgtata
    20641 taccattgct ccacatccag agtattcatt ctcacatctt tttatgtcca agttaatatt
    20701 ctttatcttt atatccaaat acttataatt atgtaatctt ttatctgttt ctttaaataa
    20761 tatatctttt ttgttttcca tagtacttcc ccttttttat atacttatta ttagcttcaa
    20821 atttacaata atctctttag tgcacagttt tttactaatc tcacaacttt caagaacata
    20881 ttgttctata actgtataaa aaaaacttct tctatatttt gttctaaaat actagaaatc
    20941 ttcttagcca taagaagtcc aggatttttt atacctcttt ctatttgaga tatataatca
    21001 tttgtaattc cagcctttct ggcaagttga atttgtgtca tacctttgca ttttctataa
    21061 tatttcaagt tatttttaaa cattgtttta tacctccatt actattaaga atattttgtt
    21121 cttcctcact tagattatat agaattattt gttctttatc aatagatttt agaaaaaaat
    21181 attcttttat attttattag ttttattttt tagaacaatt tgttataatc tatacataag
    21241 gtggtgttat aatgtttgcc aaaagattaa gagaacttag aaaggaattt ggattgactc
    21301 aaagagaact tggagaaaaa gtaggcgttt cccaaagagt cttagggtac tatgaaacag
    21361 aaaacagatt tcctgatgaa cacattttaa ataaattggc tgatgtattt aatgtatcag
    21421 ttgattacct tcttggaaga accttagtta aggaaaatat tgatacagta gctgcacata
    21481 gaaaaaatcc acatgaagag ttacctgaag aagcacaaga acaacttaat gattatatag
    21541 aatttttact aaataaatat aaaaaaaaat aaaccatatg atgagcagtc tatttttctg
    21601 ctcttttaat ataatttaaa ggcaaacata cattctattt tagggggatt tttatgaata
    21661 atttggataa gctttttgaa ttagcttctc aagaagagat aataattcat tataccactt
    21721 atattgcagg tgatttagaa ggactatata taaacaaaca tggtattaaa attatatcat
    21781 tactcagtaa tttaaaacaa aactcaaaaa aattgacatc catcttagct gaagaacttg
    21841 gacatcattt cactagcctt ggatactatg tatcctctta taacgattat tatacaaaaa
    21901 ttattataga taagtgcgaa aataaagcct taaaatgggc ttgtgagttc ttaattactg
    21961 aagaagatat aataaacata attaactctg gcatcacttg cgtttatgaa atggcagaca
    22021 tacttaatgt ggatattact ttctttcaaa aaagattaga atttctatca ttaaaaaaac
    22081 agtctttaca acttggaaat aataaatatt taatattaac taatttgccg tatttctaca
    22141 tatttgatcc aatttcataa taaaaatagt agtaatttta actggcttaa cagtaaaatc
    22201 tatatattta ttttaacaaa ttttggtttc tataaattca atattaatat ataataatga
    22261 tatcatatta tttaaaattc taaaaacaaa tatatgcttt tgttaagaaa ggttgtttga
    22321 tgaatgtata aagaaaagtt gtttgataat tattttaagt tcttagcact attattttgg
    22381 ccaattatgt ggtataagtg gatagtaatt tcaaatggta ctcttgaaaa tatgttattt
    22441 accatatatg caatcgtcgc aattgttttc atcattttat attcggtgtt tatgataaaa
    22501 tataaagaca ttacacaaat agatttcttt tacaggataa gtactctact tgcttttata
    22561 tttacacttt ttagcttttt aatataccca aagagtttat ttttcttgta tttaaagatt
    22621 atttttactg gaatatacct ttattattca atagtaaaaa ctcttaaatt taaagatgac
    22681 gaaggtgtag tgggtattat gagctcactt ttattaattg taataacttt gttctactaa
    22741 atttaattct atttgactta atatggcttt aaatattaga catctacatt attttctgat
    22801 attatttaag atggtagtct tatattaata atgaaataca ctatgaagga gttaactatt
    22861 atggaaaaaa aagttttagc aacagttgga gaaaaagaga taactaatat tgatattgaa
    22921 aatgcattaa aatcattaga cccttatcaa gctatgcaat ttaaaactga agaaggaaaa
    22981 aaacacttat taaatgattt agtgaatcaa gaattattct tcttagatgc taaagaagaa
    23041 aaattagatg aagaagaaat atttaaatta gaaatgaaaa agatagaaga aaatgtatta
    23101 aagcaatttg ctataaataa agttttatct agtgttaatg taacagaaga tgaaaaagta
    23161 aaattctttg aagctaataa gagctctttc tctaaaccag aaagtgcaac tgctaaacat
    23221 atattagtag atagtgatga aaaagctaaa gaaatacttg ctcaaataaa atcagaagaa
    23281 atatcttttg aagatgcagc tcttaaacat tcttcttgcc catctaaaga tatgggtggt
    23341 gatttaggaa cattcggtag aggacaaatg gttcctgaat ttgaagaagc tgtgttctct
    23401 atggctaaag gtgaagtaag tgaaccagtt aaaactcaat ttggatacca tataataaaa
    23461 ttagaagacc ttcaagaaag tacagagtct acatttgatg aagttaaggc tgaagttgaa
    23521 aaaagtttat tatatcaaaa acaaaatgaa gtttatggaa ataagataaa tgctcttaat
    23581 gctaagtacg gaaaccttgt aaaatacaac gattaatcat aaggtcatat ataaaaaagc
    23641 tgtcttttca caatatttat tagaaaagac agctttttat attaaagttt aaatattcag
    23701 tttcatatta tcattctttt attgtaaaga actcagctta aaagtttaaa taagattttt
    23761 aattatcatt agctaaattt ttatatccac gtattatctc ataacagtaa cctattacat
    23821 ctcttctttt tattatccca ataaatgtgt cttggtcatc tataacagga atgaaatttt
    23881 gatttaaaga ttttataact aaatcttcaa tatctgcgtt gatggaaaca ggagaattat
    23941 ccatttttct atttatatct gtcactggaa catcttccaa tccctttaag tccagattta
    24001 aatcattttt taatgtccat aaaaaatcac cttctgttat agttccaaca tatttaccat
    24061 ccttacttat tattggaatt gcagagtatt tgtgatattc cattttttct agggcttgcc
    24121 ttatagtgta gtcttcatat atatatgcta cttcactttt tggtgttaga aagaataata
    24181 tgttcattac actacccacc gttactgaaa acttataaat attactcaat atttataatg
    24241 ttactttttt gcagtaactt tctcctttct tattttctaa gtattcttaa tactatacgc
    24301 tgtaacaagc gtttttcctt tgctcatttt aatcttatta tgctttatta atatttttat
    24361 atggtctatc agtatcactt attctctttt tagaaaattt actaaaaatt aattgactta
    24421 aacttagcca taataatttt actctacact attaatttta acatatttat ctcattatta
    24481 atgattttat tttaataatt ttaatttatt ttgctattaa ttttatttac aaaaactgaa
    24541 aaaaccgcta aatgattagc ggcttatctc tcatattact agaacactaa tatatatgaa
    24601 tttttaatta taatctaact acgttagcag cttgaggacc tttagctcca tctactactt
    24661 cgaactcaac gctttgtcct tcttctaatg ttttgtatcc atctccagtt atagctgaga
    24721 aatgtacgaa aacatctcct tcaccttcta cagatatgaa tccaaatcct ttttcgttgt
    24781 taaaccattt aactacacca gttttcatta ataaatcctc ctaaaaaaac acaaatattt
    24841 agctactctt ttctgtaacc ttattactac tctatcatat atagctatgt ttgtcaatta
    24901 ttattatttt tatacattaa taatcaattt ttttacttta taattttaaa tgattattat
    24961 cttttacact ttatttttct ttataatttt ttaataattt atttttatta tatggaatct
    25021 tatggactta tcactccaac ctaaatccca tggcaccaac attctatcag tattatgaca
    25081 agttaccaaa ggataccctc ttgagtctaa tcctgtcact gtcgaaacat gtgttattct
    25141 gcctttcttc tcataaccaa caaaatcccc tggacgtaaa ttataagctt ctttgtatac
    25201 ttcttcatag gaacctttag caattaatga acctcttcca ctatacagtg cataattttt
    25261 aaaagcttgt gcatttaccc atgcttttgt acctgctctc tcgctataat tccaaattga
    25321 gttctttttg aaccttccac tttcaaacat tatctgtgat gcaaagtttg cacaatctcc
    25381 accttctgga ttataatccc tatagtttgg attaaatttc ataccatgtt cttcatcagc
    25441 tgcggctcca cagtatttat gtgcataatc tatagccttt ttttgttcat ctgtaagctg
    25501 taactctaca ccatcttgtt gaccaatata atttcttata tcatcagatt ttatattttc
    25561 taaatttaaa gagtctgcaa aagggtctgt ataccactct ttagttatga catattgatt
    25621 atctataatt tttacattta gataatgata tgtacctatt ctaaaggcat tttctttgtc
    25681 cttttgattt tcatatgaat acttatattc agtcgatact gaacagatta taccatagag
    25741 gtctttttct ttttctttta tttttttaat cttaaccctt gtctttatgt ctttaaattt
    25801 tacaccttgc ttactagacc aattttctaa atacttcatc ttttttactt cattttcata
    25861 agcccacaga ccaaattttt tatccgtctc ataaagacct tttaaaatgt tttcattttt
    25921 ctctaatatt gccttatttc tgtaatcaaa taagttttca agcaatagtt tatattggtc
    25981 ttttacatca tcaccttcaa catttgcaga ttccatgact ttttcttcca tatttttcat
    26041 acttaaatca ttgattaaaa atacacctgc tgtagtagct aaagacaaaa atacaaatgc
    26101 cattatacct ttgatgtact tattcttatt tttcagtatt ttcaaagcta cacctcccaa
    26161 atagtacacc ttacaaatca aattacaaag aatattctgt cacatatagt aattttcata
    26221 tttaataatt ctctatttta ttatgtccaa tattaaaaat taattgtatc aatacactgt
    26281 tttttttaca attttttatt ttataatcta cttttttctt ttttttatag taatatatat
    26341 gtatataaat attttggtat cattataatt aatacatact aatggagggt tttttatgca
    26401 aaaaaaagtc gcagcaatta atgacttatc tggaatagga aaatgctcat tatcggttgc
    26461 tatacctata ctttcagcat taaaggttca atgttgtcca tttccaacgg ccatactttc
    26521 aagtcaaact ggttatcctg aatttacttt tttagacttt accgatgaaa tggtaaaata
    26581 ttcaaatgtt tggaaaaatt taaaagtcaa ttttgatagt atatatagtg gttttttggg
    26641 ttcaaagcat caaattgaga tagtggctaa ttttataaac gattatccca atgcttttat
    26701 cgtagttgac cctgtgatgg gtgataatgg agttatgtat ccaatattca cagaagaaat
    26761 gagacatgaa attaaagaat tagttaaaca ttcagattta actactccta atttaacaga
    26821 agcttgtttc ttaactggaa atgattatac caaaagcgat tataatagag atgagcttat
    26881 atatattgca aaatctgtat ctgatttggg acctagcaaa gtcgttatta ctggtatatt
    26941 agaagatgat aatatattaa atttggctta cgatagagat aatgaccatg tattctttac
    27001 atcagtcaaa tataataatt gttcttatag tggaactgga gatatattca catctatact
    27061 ttgtggtatg ttagtaaata aacatgattt gggagtagcg gttaatactg caacagattt
    27121 tatatataaa actataaatt acacatctca atttgatact gatagaaatg atggagttat
    27181 gtttgaaaac tttttatcag atttaactaa tatataaagt caaaaaaact atccatttta
    27241 taaataccta taaatcataa aatttaggta tttacaaaat ggatattatt tactaaacta
    27301 ttggtatttc aattgttacc atagcaccac cagttttcac agaattttca atatataaaa
    27361 tcccattatg tttttgtaca atattattta caatatagag tcccattcca taatgagatt
    27421 tagaatttct actcaaatct cctgtataaa actgttctgt agcttttgac aaatctgctt
    27481 tagaaaaacc acttccacaa tctgtaacta caaattttaa gtatttatta cgtactgata
    27541 cacttatgta tagtttactc tggcttggtg aataatctat agcatttgaa attatattca
    27601 taatagctct gaataacagt tctctatcta tagttatatt tttaggcaaa ttatcttgtt
    27661 tataaaactc cacatctaag tttttagtac tagctaatgc ttctatttgc actaagacat
    27721 cttttataaa ctcactcata ctgattgctt ctttatttaa tatatagcca ttctctgttt
    27781 ttgatatatc tattaaagta ttaatatatt tttctatttg agtgacaccc tttgagatat
    27841 attccatgta ttctaataac tcaggatttt catttatttc aattaataag tcagtatttc
    27901 catgtataat tgttagtggt gtttttatat catgtgccag tgcagatatc tgttcctttc
    27961 tattttcttc tagcatccat tgtttttcta aagaatcttt taacgccaac ttcattttat
    28021 ccatagaaaa aaggacatta ttaatttcat aaattttact agattctaca acaaaatcta
    28081 aatcctgttg ttctattttt tctgtagtat tttttaaaag ctccatttca atattaaatt
    28141 tcttgccaaa tacttttgat aatataacaa tttcaataaa aaatattata ctaaaaataa
    28201 tcatacccaa tgtctctggt tttggtaaat atgctcttaa aattggagaa ctatactctg
    28261 atactatttt gtattttatt ataaaaactt catctttctt aaaaaacttt atataacttt
    28321 ctgaatatgc atgcctttct tcaatatctc tcattaaatt ccatacttct tttgattctt
    28381 ttttattaaa ggtccctgaa attaaactcc catccaaagt ataaacccca tactcatata
    28441 tacttggaat tatatcttca gttacttttt cacttgaaat aatctgttct ttatatttat
    28501 taaattgttt ttctgcataa ttagctggta aaattacacc tgatgataat aatacagaaa
    28561 atgataacat taaaaggagt acaattgaaa ttgtcataat aaagaataaa gataaatatt
    28621 ttataaaaat agttcttaac ttttcaactt ttttgttgtt agatttttta cctccaagca
    28681 tttgtaccct gtcctccaat agtttaatta attcataatt aatatctaac cagtttttta
    28741 tttgattatt ttatatcttt ttattgctaa gttatatttt aacataataa tataaataaa
    28801 cagtcaattt ttactctatt cttctgattt tttaccttcc catttagaaa accaaataca
    28861 taaaattaca aatgctaaaa ttgttacaaa accacacatt aaacctgttg attgatatgc
    28921 ttttattgat tcaaccttta tatattcaac cgttttagaa ctcgaaaaag acgaccaaat
    28981 agttataaat ctaacacccc atgcacaagg aaaatactgc caaataccag aacccaatcc
    29041 agttaataat aatgctgaaa gtaacatttc aactatacct actccaattg atgttccttt
    29101 tccgaacctt aaacttaaga ataaatgtaa tatgtattca aataaattac acccaattaa
    29161 aattagaatt gaaatcatat aaaagcttga tgcaaagtaa gccttagata taccaaattt
    29221 aaaacctaat acagacacta atgttgctac aaaaccaaga catactaaga ttatatattt
    29281 acttataaga gtaagatatt ttggattaga tgttgttaac atatgtttat aattccctgc
    29341 ataatattct tgttcaacaa acattgaaca cacaacacct gacaataaag gaaaagctat
    29401 tgctataact tgtaaatatg ctactatctt tagtatatta tcagctcctg aaaccttata
    29461 ataccataaa aacaatccca tccccattaa agcaatgcaa atgtgcatta gtattataaa
    29521 atttctcttt aattttatta aatcagaaca aaatattctt attaactgtc tcatattact
    29581 tcacctctct attattgaac caaacccttg tagctaaagc aaataataca aataaaacaa
    29641 tagatattaa tactcctggc aaaataacag cattatttaa aaattcactt ccctctggta
    29701 caggaagacc atttggcaga atatgcaata caacagccat taatcttgaa ggaatcgaat
    29761 atggaaacat ataccacatt tctgtagttg agaaaacagc aactccaaca atagtaaaga
    29821 acatatttat tagaactgta acaaatatac caaattttga agctaaaaac atacaaagag
    29881 gaatctgcca cataaatagt aaaataatta atatagttgc aatgatattt tgacctattg
    29941 aaatagtttc tttatagatt atcccaacaa aaactacacc tattgcagaa ataattgatg
    30001 ctataaaaca acaaataata cagtaaatca tttttccagt ccaaacgtct ttcatttcaa
    30061 ttggcaatgg taaaatacca cgatatttac ttttttcatc ttttaatact attaaagaag
    30121 atattaatgt taatgctcct ggaagaaatg tcgtatacca ccagttgaaa gcagcatttt
    30181 gccctccacc tagtccacat aaaagtatac aaaacactgg aactatccat atcaacttca
    30241 ttccaaaagt tcctttcatc ttttgaaatt ccactctaat aaatctttcc atattactgc
    30301 cctctctttc tattttcagc tacaactttc ataaaaagtt tttctaagtt ttgttctttt
    30361 gatacttctc cttgataacc cattactcca ttagaaatta ttcctatttc atctacaact
    30421 tgttcaactt cagacaagat atggcttgat aaaataactg taattccttg ttttggaaaa
    30481 gaacgaatca attctctaag ttcttcaata ccaatagggt caagaccatt tgtaggctca
    30541 tctaagatta aaagttttgg gtgatttaat aatgcaatag caataccaag gcgttgcttc
    30601 atgcctagag agaattgacc tgaacgcttt ttaaaggtat ctcttaaatc tacagtctct
    30661 aaaacttctt caatgcgaga gtcaggaaga ccaagtaaag tagtatggac ttttaaattt
    30721 tctcgtgctg ttaaattctc atatatagca ggagactcaa taagagaacc aatatttgat
    30781 aaatcctttc tagtccatct atgtccctca aacattattt ttcctgaagt tggatttaaa
    30841 atcccagtta tcatttttaa taaagtagat ttcccagctc catttggtcc taataatcca
    30901 tatatagaat tagatttaat tgctagtgaa atgttatcaa ctgccatctg ctccttaaat
    30961 tttttagaaa ctccttttac ctgcaaaata taattactca taattttcat cctttctttt
    31021 gtcaaactta tttacaagaa tcattctaaa tcataaatat aaggatttta taagaattaa
    31081 aaaacaaaaa aagatgtgtt aaaaacacat ctttttttat cttaattcta ttaattttcc
    31141 attgtagtat atctctactt cttcaatcca aatgctatta tcttcttctt ggatgacatt
    31201 tttaataata ttatttatag tatatattat attttgttca ttcaaataat catctttcca
    31261 agaaactcta ctttttttat ctggttctat caagtttatt ttaacaagat tatctttttc
    31321 cttaacttct acttccatgt ctaaaccatt aaaatactca tttgataatg tatctaagat
    31381 taatcgcagt ttatccttaa gtgaactttc ttcatcaatc tgtaagtact ttactatttt
    31441 ctcatccttt gaaattatct ctataggtat tttagtagtt tttttagaat taatcatttt
    31501 actagttgtt tcttcttttg catcttcatc cttctttaca actgctgatt ctttagaatc
    31561 aactagctca actgcactga tgctataatc catacatgcc gtactcaata aagtagtagc
    31621 aattattaaa aaacttactg ctattttatt cataagtatc cctctcctaa ttctctttcg
    31681 atacttatta taccatagta tagattatat ttttatactt ttaaatcaat ttataaatta
    31741 ttatagtaaa ttatttacaa ttctaagcta tttgaattta tttttatagt atattctttc
    31801 tctaaattcg acagattttt attcaaaagc ccttttattt ctctcgtatt tctatacttg
    31861 ttgctatgtt cttcactatg ttcataatca tatgaatcat tgtcatattc ttcatcatta
    31921 ttaaattctt caattgcttc ttttgacttt atatcacaat taaatttata cttatttact
    31981 ggtatatcaa gtcttacctt agaatttgga acttctatgt ccaggaactt agcaattggc
    32041 atatcagagt acaagtaaaa ttcactttca taatttgaat ttctacctat tttctctttt
    32101 atatttacat tttcaggtat atactcttct atattattga taaatacatc ttcattctct
    32161 gaagatatat acaatgactc tgattctatg tttacatttt taacgcctaa aattgatttt
    32221 gttgacaagt gaagattgtt attagatatt aagtttaggt tttctaatct atttactttg
    32281 ttctctgtta tagctctact aaaacctcca taagaagttt tatatgttat agtatttgaa
    32341 gctacattat cgtatacaaa taagtgtgag cttatacttg atgcatttat gtctacatta
    32401 tttggtacat atattataat ttctctataa tcatgtgaaa acatactatt tatagatgta
    32461 tttaataatt ggtcgaaatt tttaatcttt ttaattttat tttcatattt tctttcacct
    32521 tttacaacta aagtcttatc ttttaaagtt acattgtatt tgtaaaactc attatttcct
    32581 cttgttgtta cacgcacatc ttcacttgaa cttttcttta ttgtaacgtt tatattgtca
    32641 acagctatat ctaacttgtc aatatttact ttttcttgat aaagtttatt ttctttttta
    32701 aattcagctt ccttttctaa accaaagttt attaggcttg gcataactaa aatccctgac
    32761 catgttgtcc ctattatacc tattaccaat aaaaccgcag caaatatagc aagtttttta
    32821 ttcatgttta tcttctcctt tcttattttc tatatcttca aggtccttat atatcgcctc
    32881 aaaatttata ctatcatcat tatattcatc agtttctttt atgctttctt cttctctatt
    32941 gtagtaatca ttattatatg aatcttcatt aatgtcttta ttcaaatctt catcatttaa
    33001 agtagctttt tttctttttt ccatcttttc atttgctttt atataaatat tttttctctt
    33061 tatccaatgt atataattat ttagtaattg tttacctagt tttatggcaa ataataaaat
    33121 ttgccatgca agtatctcaa gccctacaaa tgctatggtt gcgaaaatac aaaacatcat
    33181 aacatctgga accattgcta taaatttaat cacaaatgga attaatataa caaaagcaat
    33241 taatgagcat actaatgcaa tagtagcaga tacaatagca catacaaacg caaatgctgg
    33301 aacaattaga aataaagata acactactaa taaccccttt ataacttgtc tggataatcc
    33361 tcctacacct tcttctatac ttggtgtaag tttatcattt atattttctt ttgatttatt
    33421 gtacttttcc ttagcttcat ctttcaagat agaataatcc ttttttacat ttgatttaaa
    33481 tctactaaat ttaccattta atatgtctgt attcattatt tttccatcta tattttcttt
    33541 tgatttacta tacttttctt ttgcttcatc ttttaagata gagtaacctt ttttcatatc
    33601 tgatttaaat ttactaaact tatcattcaa aacatctgta tttattattt tttcctcttc
    33661 aatatttttt tctttagttt gtaagataag ttcttttgct atagattttg gtgagccaag
    33721 tgatgctatt atctccatat cactctttcc ctctataaga ccatctacaa aatactcttc
    33781 ataatctctc aatatatcat taacttcgct ctcagaaaaa tcttttttta gataatcctt
    33841 taaaatttca agaaactcag ttttattcaa gttcattacc tccctcatct tcacttatta
    33901 aaatctcaac agcctttaca aattctctcc actcttccat cagattggat aaattctctt
    33961 ttcctagtga agttattcta tagtattttc tagcaggacc ttcattagat tctagtatat
    34021 atgtttcgaa atacaaatct tttgtaagtc ttcttagaat aggatatatt gttccttcat
    34081 ttacatctat tactttagag atgttatgta ctatctcata tccatacata tctttcttag
    34141 atatgagagc aagtacacaa atttctaaaa caccctttct aaattgtgtg ttcatatact
    34201 ccatcctttc tttaaataca cagtattgtg ttttacaagg tactatgtta aaacttctta
    34261 tatcctcata ataacacagt actatgttct gcacaatagt tatttttaaa tattcttgtt
    34321 gattaaatcc ttctataaat gtataatact ctaatcagat atattttatg aaagtggtga
    34381 taaattggct aaaatgaaag atgataaaac aatattaatc ctaactgctc aatttggtgc
    34441 tggacatata agtgctgcaa aagcagtcaa agaatgtatt attgaaaaat atagtaacta
    34501 taatgttgtt atacaaaact ttataaatgc aagtattccc atgatgaata agcctatggt
    34561 aaaactttat gaaaacaata caaaatatac tcctggatta tataattact attattactt
    34621 taagaaatcg tttgattcaa gacatgattt atctcataaa ttatatacac ctaaactttc
    34681 tgagtatatt gctgatataa atccagattt gattatctct acatttccac tagctgctgc
    34741 ttgtgtaaat aactttaaga taaaaaatcc tgatataaat atacctacac ttacagttat
    34801 aacagatgtt gtagatagta tggaatgggt ttttgaaaat acagatttgt attttgttcc
    34861 ttctcctgaa ataaaaaata gattttttca gaaaggaata aatcctgact ccataaaggt
    34921 tactggagtt cctgttgata agagatttca aattgaaagt aaagaactat gttgtgataa
    34981 atatagacta ttacttctag gtggaggaag aggtctattt gatatagatg aggattttat
    35041 gcattggata gatgaattta ttgaagaaca tagtaattct atagaaatta caatagttac
    35101 tggtaaaaac aaaaaattat atgacaatct aactcataaa aaacctctaa aaaatattaa
    35161 agtacttgga tttgtaaacg acatgtataa tctaataaga gaatgtgacc taatgctaac
    35221 taagcctgga ggagcaacta tatttgaagc tattcaatca caaactccag tactcgtaaa
    35281 aatgccaaaa gttggacaag aaattgaaaa cgctaaattt ataatagata agggtcttgg
    35341 catgatttat agtgatgacc tagaccttaa aaacatattt tataaattag tatcaaatga
    35401 atttgattct ataataaact ttatgaaaaa aaatttaaaa gaatttaaaa cagtcattca
    35461 tccagagaaa attgctgatt atatatcaga attaatagat aatcattaca gttaaattgt
    35521 actattaaaa aatttcaaac ataaaattaa aggcaatcaa gtatgatttt acacttgaga
    35581 atttactcat agtgtttaat aatacttgat tgcctttatc atatcaaaat caaattgtta
    35641 tattagtata tatttttctt gtactgcata tacagttgtt gtatttcttc catacttctc
    35701 tttaattcta tttgtaaatc tgaagcagtc tggtaatcct tattttctat agcttctctc
    35761 actctagtga atagtgattc tctagttaaa gtatccttca taatattgaa ctttaaatcc
    35821 gatattgagt tccaaactac ctcatcaagc gcatccatat tttcttttgt gtgtatcttg
    35881 acaatacttc ttattttttg aatcccagcc tctattattc tagtttttaa tttcttttgc
    35941 cacttatcta tagctccaat cttaaatgat tctattatac tatcagtaaa tacatcacct
    36001 tgttttaaag attttaattt tgaagcatat atttctaagt ttttcatatt ttcataaact
    36061 gtagctggtg gtattccaaa tcttgcattt ctttcttcca aactataatg ttcaaataca
    36121 tcattttcat ctctatatgc tctatctttt tctaaataga aactttcttc acctacattt
    36181 ttagataact ctttttcaag ttcttttgta ctaagaccac tcttagctgc tgcttttata
    36241 ccatccaaca ttgtttgata acatcctgct attactaaat acgtgttaga tagagggctt
    36301 ggtgacctta attcaaatct tacagtcttt ggatttttca tatctcttat taatccaaca
    36361 agcactgacc tatttcttga tggaatctca taactatgcc ctaaagatgt aactatacat
    36421 acaggagctt caaatccagg aactaatcta ttaaatccat cattagaagc tgttacaatt
    36481 gggtttaata cttcataatt gtaaagaagt cccataagag ccccatatcc tagttcactt
    36541 agataatctt cttttaaatc ttttggagca aatagatttt ttatacttcc atcctttaac
    36601 ttcgcactta ctcctacatg tgtatgtcca ccacttcctg ctactccatg aattggtttt
    36661 gctttaaatg taacttctag tccatgtgat gtaaatacat cctctataac atctcttact
    36721 aataattcat tgtctgctgc ttgtaatggt gtagaaaact tccaagaaac ctctaattgc
    36781 tccatagcat ggtttgttct tccatctata ctaatagaac tttgtattcc tccgacctct
    36841 ttgtgagcca tttcagggtt tacacctagc ttttgaagta taattaaact tttctctaaa
    36901 caagttctta taataccatg agttcttttc cagtattgct cttttaggct ttgtgataca
    36961 tataatttct ctaagtcagc tttatcctca ggagtattaa cccagaactc aagttctgta
    37021 gctgctgtta acattatttc ctctatatca tctactgaat ctattcctat gttatttatt
    37081 acatgtggat attctctaaa tatttcatac attgactttt taaagtactt atctgctttt
    37141 tgaagtactc ctctagaaca tactttttta ttatcatgta ttaaaaatgc aggtatcttt
    37201 aatgttccaa caggtagacc tacttcttcg tctatatatt ccatattgta atctacatac
    37261 caatgacaac ttttatctgg cattaaatct actttcgcat tatttaatgt agctatatta
    37321 taaagctcaa cacttgaacc atcagtctgt atagctgatt ccaaaaattt atctatatcc
    37381 tccaaaaata attctacagg aatcttttca tctgttgcat ttcctcctaa atcaaccccc
    37441 attaaggaaa caaatcttat atttttattg ctttttagga tttctcttaa ttcttcatta
    37501 gtgtgtttat ctttctctat tacgtataat agactatcca tttttccact ctcccataat
    37561 ttttatgtat attttatttt gttaaataca agataaattt cattttcctt acatttatga
    37621 atttttcttg caaattattc gtgttttatt cgtgttttta tttatattat tatatatcaa
    37681 gtataaaaag ttcgcaatat tttttattgg aaaaaatatt atacatacaa tagtttaaca
    37741 tttttacaaa tatggggtat actaaaataa aagtattttt atttgaaaat ttaattttat
    37801 ttataatata atctatagta aaagaaataa taaaaaaatt taggagataa actttatgga
    37861 atacataatt ttcgatttgg aatttaacca aggatttgat aaaaaactca ataaaactgt
    37921 atccaatgaa aagtgtcctt ttgaaattat acaaatagga gcaattaaat tggattctaa
    37981 attcaatata atagatacgt ttaatagtta tgtaaaacct acaatttata aagacataca
    38041 tccctttatt agtcgtatga ctaatattaa aaattctgat tttaatgatt cgcctacttt
    38101 ccctgaggtc tacaacaatt ttataaaatt tatatcatca caagacccta tattatgtgt
    38161 ttggggagct ggagatttaa aggaattata caggaatatc aactatcaca agctaccatc
    38221 aaattcctta cctaaatctt atattaatat acaacaacat gcatctaaat atttcaataa
    38281 tccagcagga aaaagcattg gcctacaaaa tgctatttct atattagaac ttgatgaaaa
    38341 aatgtcttat cacaatgctt taaatgatgc atattatact gctaaagttt ttattaagat
    38401 atacaatcca agtatagtcc cagatatata tctttataca agtataaaac ctaaaactat
    38461 aagatattct aacaaaaaaa gagttgatta tgataagtta tttgatgaat ttaggaaaat
    38521 tttaaatcgt gaattaacta aagacgagaa aaaaatcatt aacttagctt ataatatggg
    38581 gaaaacaaat caatttacat tagaaaatgt taagcaaaga aaaaacaaat agagtttatt
    38641 tagataaaaa ataggcatgt agattaaaaa actagttaac tttaaattta tatctacagc
    38701 ccaattttta tcataattaa tttttatttt atctctaatt caactggaca atggtctgaa
    38761 cctaatattt cagtgtgtat atccgcactt actaatctat cttctaattt tttggatgca
    38821 caaaaataat ctattctcca ccctgcatta ttttttcttg cattaaatct atatgaccac
    38881 caactataaa caccttcttt atcaggatta aaatatctat atgtgtctat aaacccactg
    38941 tctaataatt tagaaaactt ctctctttct tctttagtaa aacctgcatt tttcatatta
    39001 tttttaggat tttttaaatc tatttcttta tgtgcaacgt tcatatctcc acacacaatt
    39061 acaggcttat gattatctag tttggttaaa taatctataa agtcgtcttc ccatctagtt
    39121 ctatattcta gtctctttaa ttcagattga gaatttggcg tatatactgt aacaaagtaa
    39181 aaatcttcaa actctagagt tatcactcta ccctctttgt catgttcttc tatatttatt
    39241 ccatacatta cttttaaagg ctcttttttt gtaaatatag cagtacctga ataacccttt
    39301 ctctcagcat aattccaata ttgaaaatat cctggtaaat ctaactctat ttgcccttct
    39361 tgcaatttag tttcttgtaa acaaaatata tctgcatcta cttctttgaa aaagtctaaa
    39421 aatcctttcc caacacaagc tcttattcca ttaacattcc atgatataaa tttcatactt
    39481 atactcctca tctaaacatt gacacactcc ccatagctaa agatgggggc ttttcaacaa
    39541 ttttattgta aattcttaat tcacttttat tttaacaatg ctatactata atttcatcta
    39601 aaattactat ttacttaata cctaaaaata tatgttttaa tcaattttta acttttttgt
    39661 taaatcttca gggctcattg aagaaacata catatttgtt ccaagttcaa atcctccaaa
    39721 attatatttt cttgggatta tgtgcatatg aacattaaaa tacgattttg tttcaatctt
    39781 tgttggatga gtgtgaatac ataaattaaa tggcatatat ccattgatat tatataactt
    39841 cttaaacaac ttttcaaata ttgtagaaag ctcctgtata ttattatcat ttaactcacc
    39901 aaactctatt ttatctttaa atattattct tacttctcca ctatacttgg ttgcatatgg
    39961 tataagtacc aaaaaagctt ctccattata aacaactctt ctatctaaac ttatttcatt
    40021 ttctattaaa tcatcataca aagagctttt attgctgtca taatactcct ttgaaaccaa
    40081 cagttcattc gtaatttcag gtggaataaa agacatagac aatatttgag catgtggatg
    40141 catcaaagat gctcctgcct ttctcaagaa gtttttgaag atactaacat actctacttt
    40201 atcatctttc gacaaatctc taaatctatt tctatacata aaaaaaacat cttcaaattc
    40261 tttttgactc atgttataaa aactacgatt atgtttatcg ctctctatca ttacttcatg
    40321 aacaccatat acatctcttg ccatatcatc aattatagga aatttattgt agacagattt
    40381 tacaagccat ccttcttcac tttctatttc aaataatgca tcagttgcat gagcttcatt
    40441 tcctttgcaa aatgggcatt cttcatcata ttcattagtt tcttcatctt catttgggat
    40501 atctgctgta tctaaaggtc tttttaacct atctgtagca aatataacaa catcattggt
    40561 aattggatct attcttaatt ctttcataat taacacctct tagttaatag cctttaatat
    40621 aattatatga tatttatcgt atctgttaaa taaatttata atatttttta taattttgca
    40681 tatttattat tatatatttt gtctatagct gaaagattat aactaaaaaa tcactatcag
    40741 tatatcagaa tattatctta ctataaatta atttatgaat acataattta gtggcataaa
    40801 catgggtatt ttactataat ttgttaaaag tttattttaa agctatatta attttttttg
    40861 atgattttta cccataagta tgttattatt atcttgtaaa gatgtcttgt attcaaacat
    40921 acaatttaca taatacaata tacgtattta aattaaatag ggggtattaa aatgaattat
    40981 tcaaacagag ttagtgcaat gcaagcctct ccaatcagaa aacttgttcc atttgcacaa
    41041 gcagcaaaag ataaaggaat caaagtatat cacttaaaca taggacaacc agacataaaa
    41101 actcctaaag gattttttga tgctgttaaa aactttgata gcgaagtctt agaatatgct
    41161 acatcagaag gtattccaga attattagaa gcacttcaaa attactataa aacttacgat
    41221 atgaattttg aaaaagatga attattagta actaatggtg gtagtgaagc tttacttttc
    41281 actatgatgg cagtttgtga cccaggagat aatctattag ttccagaacc attctataca
    41341 aactacaatg gatttggtca atctgtaaac gtagaagtta atgctgttac aacaaaggca
    41401 gaaaacggtt tccaccttcc atctaaagaa gaaatattgt ctaaagtaga tgataaaact
    41461 aaggctataa ttctttctaa cccaggaaat ccaactggtg caatttatac aaaagaagaa
    41521 cttaatatcc tagctgaaat agctaaagaa aaagatttat ggataatagc agatgaagtt
    41581 tatagagaat ttgtttatga tgggcttgag tatacaagct gtggtaactt agagggtgta
    41641 caggatagag ttataataat agacagtgtt tctaagagat acagtgcttg tggtgctaga
    41701 attggttcta tcgcttgcaa aaataaaggt cttatcgctc aaatccttaa attatgtcaa
    41761 ggaagacttt gtgttcctac attagagcaa ataggagctg ttgagttata caaaactcct
    41821 gtttcttact tcaaagaagt taatgaagag tacaaaaaaa gaagagatgt attatacaat
    41881 gaattaatga aggttgaagg tgttatatgt aaaaaaccaa ctggtgcttt ctatatagtt
    41941 gctaaattac cagttgaaaa tgcagaagat ttcacaattt ggatgttaaa agaatttaac
    42001 aaagataatg aaacagttat ggtttgccct gctgaaggat tctatgctac tccaggttta
    42061 ggacgtgatg aaataagatt agcttacatc ttaaatgaaa aagaccttca tagagctgct
    42121 acattattaa aagaaggttt agagcaatat gtagctttac aaaaataatt ttttaaactt
    42181 aatttatata aatttaatga tataaaatgt ccccttttca gatattattt gaaaagggga
    42241 catttcttaa ttaatatatt aattaagact tagacctgac tatttttcat tttctttata
    42301 tagtgttggc cagtaatctt tattcacttt aattaaagca tctaaaactt gtcttgcctt
    42361 agttggagag attatagttc tatttaatgt taaagataaa atagcttttt cataagattg
    42421 ttcaaagtat gcatcaactg ctaacttttc ataagctagt tgaccttcta acagtccctt
    42481 ataaaatgtt ggtatattac ctacatgtaa aggtctcggt ccattctttg taagaatcgc
    42541 agctacttct accattgcat catcaggtaa gttgctaata gccccattat tttctgttat
    42601 aactatggct atttggtgtt tgttatgagc tattgcttct gcaacttcaa ctataaattc
    42661 cccatgagca tcattatgca ctaaattaga acctatagat gttccttttt ctataacaga
    42721 tttacattct tcaaatacac gcttttctct tccattcata acttctcttg ctctagtaca
    42781 atttatattt gatttagcta cttttttatc aggatataga taatactgta catatgtatt
    42841 aggtaagtaa tctggtacat cttccaacat atctttaacc attccataag tgtctaacca
    42901 agatttatct ctttgttctg catcaacagg ttggaatcca gagtttgcta ctaaatcttt
    42961 tatcttaggt accaaatctt caccattttt atcatacaaa tgtgtaaacc atccaaagtg
    43021 atttaatcca aagtatacag gctcaaatgt tcttgaatcc atgtctaaca atcttccata
    43081 agaccttaac aagttaactg gttggtcaca catattaagt aatcttttat catctggaaa
    43141 tactcttttt aatgcttctg caacaatagc agcaggattt gtataattta atatccatgc
    43201 tttaggagag tattttctta cagtcttaac catttctatc atatcaccta tagaccttaa
    43261 cccataagca aatccaccag ctccacaagt ttcttgacca accagtccaa attgtagagg
    43321 aatcttttca tctaattctc gcatcttaag accaccagtt ctcatttgaa caaatacaaa
    43381 atctacatct ataaatgctt catctgggtt tgtagtatat tcaagtgttg ctttaggata
    43441 ttcttcttta aataatattt ttccaaattc ccctattaat ctttgacgtt cttctactac
    43501 atctaacata gttactttat ttagaggaaa tctatcactc attttacata aagactttaa
    43561 aagtcctgga gtccaagtac tgcctcctcc aactatagct atattgtatc tttttttcat
    43621 tgtcatatta atcacccctg ttttttatta actaacttgt aagatgatta taatatataa
    43681 agttttctac atgaatactt ttaggtaatc attaaaaata gttcaatttt agtatttttt
    43741 tcaaaattgt aatttttttc atctattgta tttttcaatg tatttatgaa acatatattc
    43801 cataagagaa agtgctggca ctctagatgt atagttaaca ctattaaaat tatattgtgg
    43861 agaagataca aataatttta tatcagctat atttgataat gaattgacac ttatatttgt
    43921 cagagaaatt actttaactt gatttgtttt tgctatatta catgccttaa taacttgagc
    43981 agtctctcca gacatagata cagcaattac aaggtcatct ttagtaatat ttgatgcagc
    44041 caaatacatg agagttgaat catcatagta ttttacattt acacctatag ctgaaaaacg
    44101 ttgagacatt tcagtacata ctaatctaga agacccaaca ccaaaaaaat atatatgctt
    44161 agaattatgt atttcattta aacacatatc tatattctct acatttatta aattttgtgt
    44221 atccataaca tcagtaaaaa tatcactttg acttttagta aactccttat ttctttcatt
    44281 tctatttgaa tatgaaaaaa ctaaaaagtt tttaaattca ctatatccag aaaagccgag
    44341 cttctgacaa aaccttacta tgtatgcagt cgaattaaaa gtttcatttg ctatatcttt
    44401 aatctttttt tgatttattt tttcagaatt ttttagaaaa tagtttaaaa gagatatttc
    44461 atttgtagat aatttttttt gatttatatc taatttgtct attaggttca tttatacctc
    44521 cataataatg atttgtagtt aaacaacttt ctcatattta ttatataaat atacactata
    44581 cagcatttat cttatgttta ttatactata aaaaagacaa tacaaattat aaattagtat
    44641 tgccttttaa tatttacttt aaatagacat tgcaaaagaa acttcgtctc ttacttgaga
    44701 tacagatggc ccataaatta tttggatatc tttatcagat acttttataa ctcccattgc
    44761 acctgtatat tttttaaaag cattttcatc tactttactc atatctttaa ctactactct
    44821 taagcgagtt atacagtttg ttatatcttc tatattgtcc ttaccaccta aatatttaat
    44881 tatagataat gctctcttag tagtttctcc actttgagct atttctactt tatttgataa
    44941 aggttttaaa tcaaaattta tatctgttat ttcttctcgt cctggagttt gtaagttaaa
    45001 tctaagtatc aaaatcttaa atacattata ataaactatt gcatacacta aaccaagtat
    45061 tatagaataa atccactttg tatttggttg cataactcca aatactacta aatctattat
    45121 gccaccttga gtatttccta ttgctactcc aaatatatgc atcatcataa atgatatacc
    45181 tgataaaaat gcatgtataa caaataatat tggtgataca aataagaatg aaaattccaa
    45241 tggttccgta attcctgtta caaatgttgc taaagcacca gctagtaata tacctttaac
    45301 cttcgaccta ttttcaggct ttgcagtttt atacatggca tatgcagcag caggaagacc
    45361 aaataccatt ataggtattt tcccttgagc taaaaatcta gttgcttctg ttgaaaaact
    45421 gattgtttca gggtcactta gttgagctaa gaatatgttc aatgctccaa atacttcttg
    45481 ccctcccaca actaaagaac ctcctacttc agtaaatcta aacatagcat ttaatatatg
    45541 atgtaatcca aatggtatta aaagtctttc aagaaatcca aatacaaatg ttcctaaata
    45601 tcctaaacta gctataccaa tacctacact ctgaataatt gtatttacat atggccatat
    45661 atatggtatt attatcccca aaatagagaa tactactgtt gttattattg gaacaaacct
    45721 agttccacca aagaatccta aataatctgg cagttttata ttatagtatt tattatgtaa
    45781 tgatgctgtt attataccaa ctacaattcc accaaatact cctaattcta atgtttgtat
    45841 gcctaaagtt acagcttgac ccatttctct catcatgtca ggttcagcaa taatccccct
    45901 acaccctaac atataattta cactagtgtg taatactaaa taaccaacaa aaccagaaaa
    45961 tgcagaaact gctttttctt ctttagcaag accaacagat attgatatag caaagaatat
    46021 tgctaagttt ccaaaaagta ctcctgttat agcttttgta aaacccaata ttccttgaat
    46081 aaaattattt gctaaaaaag gcatatcact aactatatta ggatttgaaa atgctgaact
    46141 tattcctaaa aatatacctg ctgcagatat taatgctatt ggcaataaaa atgattttcc
    46201 taactgtaaa aagaaccctt tcattttttg tttcatttct atccctccat ttttaaattt
    46261 ttaaaacgtt ttctatgtca taattatata tttttatttt gtttttgaaa ctttataatt
    46321 tgttttaaaa tatttttaca taaatagtaa ttttttcaaa actgtaaatt ttttcaaaca
    46381 aagacatagt aacgttgtaa catatataaa tttaaaaaga gcttagaaaa taaaattatt
    46441 gaatataaaa tggaaaaata taaattacac aaagagatat gagtacgtac taacaaatac
    46501 gaaaatgtat aagaaattat aaaaaagtac atcaagaaaa tttaagatgt actttttaga
    46561 ttgtatttag acttataatt atatagttaa atgaataaca aagaattaat cttagtacta
    46621 ttaaatacaa aaatttttat attttttcta tctaaattct tctttgttta atatatattg
    46681 aagagtattt attgcttcta aatatttttt tctgtcttct tcagttaatt tagaaagttt
    46741 ttctcctaaa ccatcactta atttatccat aaaatcagtt gctatcttat ctccctcttc
    46801 agtaatgtca attagatatt ttcttctatc aactaaatct cttttccttg ttacatactt
    46861 cttatcagtt aaatcatcaa ccatactagt taaactgcct ttttctatat ttaacttaga
    46921 gcataaatca gtcatactta ttactccata attttttatg aaaactaatg ctcttaattg
    46981 tgttttattt acattataat ctggtgcaaa tttttttagg tcttctaagt acaggctaga
    47041 ataaatctta ggaaatgttt ttactaaaaa acttatggta tcttttacta tatagttcat
    47101 ataatcacct cggttgtttt aaaatttatt aaattatcta aataacttta tttcagttta
    47161 cattaaaaat attgcaagta caatagtttt tgctgaatgt ttaaatttaa ttagtaatct
    47221 tatcattttt ttatttttat ttatattatt cctattaaat attatttata tagctatttt
    47281 attaaattca tggtatagta agtaaagaag aactcttcac agcggtggag agaagacgct
    47341 agcccattaa aatggctatc ttatttataa ataagatagc cgtttttaat tatattttat
    47401 ctgtacaatt ctatactctt aagtctacat ctacttttcc taagtcttcc ttataatctt
    47461 ttatagcagg ttctacaact tcatttacaa attctacaac ttgttctgga gctctaccta
    47521 taaagttttt tgggtccatt atagatagta tttcttcctt gctcatttta aatgaatcat
    47581 cattcattat aagttcaatt aagttattat ccttgccttc atgctttact ctataggcag
    47641 ctttcataga atattctctt attatttcat gtaattcctg tctatctcca cctcttttta
    47701 cagcttccat aagtattgtt tctgtagcca taaaaggaag ttcttcattt acacgtctat
    47761 ttatcatatt ttcatatact actaaaccat cagttacatt tatccctatt tcaagtatag
    47821 catctgctgc cataaatgct tgaggtattg ccaatctctt attggctgag tcatctaatg
    47881 ttctctctaa ccattgagtg gcttgtacca ttgcagggct tatagattca gagattatat
    47941 acttagataa agatgctatt ctctcacttc tcattggatt cctcttataa gccattgctg
    48001 atgaacctat ttgatttttt tcaaatggct cttctaattc ttttaaactt tgtaaaagtc
    48061 ttatatcatt agtcatctta tgcatacttt gagctatccc tgatagtatg cttattactt
    48121 gataatctaa ctttctagta tatgtctgtc cacttactgc ataggaagaa tggtatccca
    48181 tctttttaca aattttctta tctaactctt ttactttttc gtggtctcct tcaaataagc
    48241 ttaaaaaact tgcttgagta ccagtagtac ctttaactcc tcttaacttc atgttgtcta
    48301 ttctatagtt taaatcttcc aaatcaagaa ttaaatcttg agcccataaa caagctcttt
    48361 ttcctactgt tgtaagttga gcagcttgga agtgtgtaaa tccaagagct ggcatatctt
    48421 tgtacttaag tgcaaattct tttaaattgt ttattaaatt tacaagcttt actcttagaa
    48481 gttttaaagc ttcattcatt tgtataacat ctgtattatc tccaacatat gcagatgttg
    48541 ctcctaaatg tattattcct ttggcttttt cacattgtaa accataagct tttacgtgag
    48601 acattacatc atgtcttact tctttttcta tttttctagc ttcatcaaaa tttatattat
    48661 ctatattagc tcttagctca tctaattgtt cttcagttat attgattcct aactcatgtt
    48721 caccttctgc aagtgcaacc cataaccttc tccatgtaga gaatttaaat tgtggagaaa
    48781 atatataact catgtccttg ctacaatatc tgtctgtaag tgggtttgag taagtagtat
    48841 atttattatc tatcattata ttagcacctt ccttaaatct tttgtcgtgt gattactgtg
    48901 tacacattag tacataaata attatatata attttacgaa ctataacaag acttttttat
    48961 tttttgttca catttatata tagtattttt tatttattac tattttttat ttatatccaa
    49021 attatctttt tcaattaagt aatccactat ttcactatca acaaaattta atacatactt
    49081 atatctaatt gcatagtatt tatcactcca tccaagctga ttttttacat cactttcatc
    49141 tgccccaagc cttaaactta ttgctgcaaa tgaatgtcta aaatctcttg ctgaatattg
    49201 agaaagtcca gctaaatcta atgctttttt gactataagt ctgacatttc tatctgttat
    49261 agaattactc ttttgagttg taaaaataaa atcatcacta ggtattatta cctctggaag
    49321 cccagaataa ttcctgtaat cttctaaaag tttaaaaaag tatggatgta atttaactac
    49381 tctttctttt cttcccttac ctaaccttac atatgagtta tcgttttcat ctaccataag
    49441 gtctttccac tttaagctaa ctaattcatt taataaacac ccagtagtaa ctagacagac
    49501 tattattact ctatctctta tatttaattt tggtaggata cctataagtt tatttatttc
    49561 ttgtatactt agaacatcat cttttgtttt tattcttgtt actgtgggtt tttctacata
    49621 tctaaatgga tttatatcta tgtatccttc tttttttaag aagttataaa aactatgtaa
    49681 ataactatat atcttttcac aagttgattt cgcatattta gtctgtatat agtctacaaa
    49741 atttttacaa tcttctttcg tagcataaat caattctttc ccttttaaaa attctttaaa
    49801 cagtataacc tttgataaat aatcgtgttt cgtgtttgga tttaattttg tagaaaaaac
    49861 attaaaagtt tcatactcat aatcatctag tatttttgta ctatacataa taactccttt
    49921 ccttttcata aaataaaata gctatacttt tactatacca tatatttccc cctcaaatat
    49981 accccttatt caatagataa cttttaagtt tattttgtta tattgtgaag gttttatgaa
    50041 aatatcaatc ttccattgtg cactacacag tactatggta ttataagtta gtaaaaggtg
    50101 gcattttttt attaatctat gaataaaata tgctactata aatttgtatt ggaggtattt
    50161 tgatttggaa acatttagtt tgcttgaaat ttttaaggca gtcattttgg gcatagttca
    50221 gggaataaca gaatggctac cagtaagtag tacagggcat atgatattag ttgatgagtt
    50281 tataaaattg aatttttcaa atacatttat aagtacattt ttagttgtaa ttcaatttgg
    50341 ttcaatatta gctgtacttg ttatattttt tagaaaatta aatccttttg atagcgcaaa
    50401 gaatattaag cagaaaaaag aaacagtccg tttatggctt aaggttataa ttgcagttat
    50461 accatctgga gttattggaa tactgtttga agatgatata gatagattat tctttaattc
    50521 aacagtagtt gctattgctc ttattgtata tggtataata atgatagggc ttgaaaagag
    50581 aaataaaaaa cctaagtata aagatttttc tcaagttaca tataaattag ctctttgtat
    50641 aggattattc cagtgtctag ctcttatacc aggtacatct aggtctggtt ctactataat
    50701 aggtgcagta ttacttggta cttctagata tgtagcagca gagttttcat tcttcttagc
    50761 catccctaca atgcttggag ctagtgcatt aaaacttcta aaagctggat ttggatttac
    50821 tggatttgaa tggcttattt taggtgttgg ttctgtagta gcatttgttg tttcaattgt
    50881 agttattaaa ttttttatgg actatatcaa aaaacatgac ttcaaagtat ttggttatta
    50941 tagaatagtc ttgggtatag tggttcttgc ttatttcttt ctattataat gtattctaat
    51001 ttaataacaa atgataagga gaaaagttat gagtgttaaa aaagaaatat ttgattggat
    51061 taagtcaata gctatggcta ttgtacttgc atttgtaatt ctacaattta taagaccttc
    51121 tattgtaagt ggagaatcaa tgtatcctac tttagatgat aaagattatc tgattttaaa
    51181 taggatatca tacaaggttg gtaaacctga aaaaggcgat attgtagttt ttaaaaccaa
    51241 tttagttgat ggagaaacag gaaagaaaaa agacttaata aaaagagtta tagctactga
    51301 aggtgacaga ataaaaatat caaattctaa agtgtatgta aatggaaaat tattaaatga
    51361 accatatata cacaataact atacttctgg agatatagat actgttgttc caaaaggtaa
    51421 actatttgca atgggagata atagagaaaa tagtaatgat agtagattcc ctgatgtagg
    51481 tatggttgat gaagatgaag ttcttggtaa ggttatggtg agactattac ctcttgataa
    51541 tattgggaaa gtagactaaa aaataagaga ctgaattggt aaagtttatt ttacaaatct
    51601 cagtctcttt ttattttctt aaattacaaa tattttttta ctaaatatct tttgattctg
    51661 taataagtct tattatatca cttgcttctt cattaaaagt tgttactata cttaggtagt
    51721 ctgttgctct actcatagct aaataaacct tatttaaatc tcctacaaag tcatttactt
    51781 gataatcttg ttcaatatca tttatagttt gattgtataa catctctaat tcacatacta
    51841 ttaccgcttt atactctaaa ttctttattg catatatatt tgctattgta gttcctacct
    51901 tcttacttat atttgtcaaa ttatcttctg cacatatata aggaattttt gcctcttcca
    51961 atgctttttt taacatatat tgaaagtaaa tggtttttcc actttttaat ttcttcttat
    52021 tgtaaggata aacgatagca acttcagaat aatcaagacc ttttttactt attaaatact
    52081 caatttccca gattacagaa cttatttggt catcaaggtc acttactttt attatatcaa
    52141 cagacttatt tccacctcta agtgctttag tataaaagaa agtactaaat tctgtatttg
    52201 gtcttaattc gttgatatat gagttagaat tatttgaaaa tttatttgta aaatctacaa
    52261 tctctttagc ttgtctataa tttttactta agataatctt atcatcaaat tctataccat
    52321 tatataattt tttaaatata ttcaatctat tagagatatt caaggagtta caagaacaca
    52381 cattaaatat gaattttgtt ttatataaaa actcccttat aaaatctatt tcatactcta
    52441 aaaagctttc tgcttcatca ataaaaatag ccttgaacat atttttattt tttataatgt
    52501 tctgtgcttg ttttacaaga ttattaaagg tcttttcgta atcatttttt aacatgttat
    52561 aatctacaat taaattgaat tttttagcta gtttaaatat aaaagaacta aatgtgtgaa
    52621 cttctaaatt gttattatct ttgtataata actctatcct ctctcgaagc tcattgcaca
    52681 attgttttgt atgtgtaaat atgactaatt tatgatgtgg atatactttt gctaatttta
    52741 tagccctaga aagcataatt gctgttttgc ctataccaga accaccttca ataagtgtat
    52801 tcccatattt aatagaaaca acatctttaa tttgaacgtc ttccatcata gttgctgtat
    52861 acttatagtc atcattgtag aatgaaattt ttttaaattt ttcatttaga tgaagtttat
    52921 catttattat atagtattct gaacatatgt ctagtaaaaa taaatttaag tttatttcat
    52981 cattctcatc attaagatat tcctccagcg aacccttatt cattatatct tgaagcttat
    53041 ttttatctat tatattatta ttaacaaatt cttcaaattc atatgtttcc tcaacttcaa
    53101 cgtatggcat aatgtaaacg tagttataac ttatatttct attcttttgt cccattttta
    53161 gtttcaaaag ttcatattcc tcattcataa cttcaagtaa ctcttcttct aaaataaaaa
    53221 atatatcttc tgttgtatcc ataaatttta taaataaaat tcttccatct ttaacataca
    53281 acaaatcggt atttattcct ttaaaaggag taacttttgg cactacttta acgtcctcat
    53341 tgatattttc gtaaaatatt ttctcgaact ccttcaattt tagggcgtct aactgagaat
    53401 ttatatttat gtattggctc acttacatta cctccttctg ttatacttaa ttataccatt
    53461 ttgaactata tttttatata tactttttat aggcaaattt agtaaataaa ttcaaaataa
    53521 atacaaataa atacaaaact gctaaaaaaa tttt
//
LOCUS       DAMJWU010000013        53245 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMJWU010000013 DAMJWU010000000
VERSION     DAMJWU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.013"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            49..858
                     /locus_tag="PC460_000960"
     CDS             49..858
                     /locus_tag="PC460_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2599857.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            992..1231
                     /locus_tag="PC460_000961"
     CDS             992..1231
                     /locus_tag="PC460_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599858.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1419..1595)
                     /locus_tag="PC460_000962"
     CDS             complement(1419..1595)
                     /locus_tag="PC460_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599859.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1649..1745)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2100..2774)
                     /locus_tag="PC460_000963"
     CDS             complement(2100..2774)
                     /locus_tag="PC460_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2599860.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(2994..3548)
                     /locus_tag="PC460_000964"
     CDS             complement(2994..3548)
                     /locus_tag="PC460_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2599861.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(3626..3991)
                     /locus_tag="PC460_000965"
     CDS             complement(3626..3991)
                     /locus_tag="PC460_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2599862.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4456..5241
                     /locus_tag="PC460_000966"
     CDS             4456..5241
                     /locus_tag="PC460_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2599863.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5338..6162)
                     /gene="pdaA"
                     /locus_tag="PC460_000967"
     CDS             complement(5338..6162)
                     /gene="pdaA"
                     /locus_tag="PC460_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2599864.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6383..9502
                     /locus_tag="PC460_000968"
     CDS             6383..9502
                     /locus_tag="PC460_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2599865.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9520..10119
                     /locus_tag="PC460_000969"
     CDS             9520..10119
                     /locus_tag="PC460_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF2599866.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10376..12505)
                     /locus_tag="PC460_000970"
     CDS             complement(10376..12505)
                     /locus_tag="PC460_000970"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF2599867.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12771..13019
                     /locus_tag="PC460_000971"
     CDS             12771..13019
                     /locus_tag="PC460_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599868.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            13185..14576
                     /locus_tag="PC460_000972"
     CDS             13185..14576
                     /locus_tag="PC460_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF2599869.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14691..16160
                     /locus_tag="PC460_000973"
     CDS             14691..16160
                     /locus_tag="PC460_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2599870.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            16225..16818
                     /locus_tag="PC460_000974"
     CDS             16225..16818
                     /locus_tag="PC460_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF2599871.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            17000..18346
                     /locus_tag="PC460_000975"
     CDS             17000..18346
                     /locus_tag="PC460_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2599872.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMFYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18528..18899
                     /locus_tag="PC460_000976"
     CDS             18528..18899
                     /locus_tag="PC460_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF2599873.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            18892..19581
                     /locus_tag="PC460_000977"
     CDS             18892..19581
                     /locus_tag="PC460_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF2599874.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            20101..20967
                     /locus_tag="PC460_000978"
     CDS             20101..20967
                     /locus_tag="PC460_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2599875.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21209..22018
                     /locus_tag="PC460_000979"
     CDS             21209..22018
                     /locus_tag="PC460_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599876.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22162..23226
                     /locus_tag="PC460_000980"
     CDS             22162..23226
                     /locus_tag="PC460_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2599877.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            23254..23973
                     /locus_tag="PC460_000981"
     CDS             23254..23973
                     /locus_tag="PC460_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2599878.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            23997..24581
                     /locus_tag="PC460_000982"
     CDS             23997..24581
                     /locus_tag="PC460_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF2599879.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24583..25932
                     /gene="pabB"
                     /locus_tag="PC460_000983"
     CDS             24583..25932
                     /gene="pabB"
                     /locus_tag="PC460_000983"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF2599880.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            25922..26665
                     /locus_tag="PC460_000984"
     CDS             25922..26665
                     /locus_tag="PC460_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF2599881.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26699..27400
                     /locus_tag="PC460_000985"
     CDS             26699..27400
                     /locus_tag="PC460_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599882.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            27426..27998
                     /gene="folE"
                     /locus_tag="PC460_000986"
     CDS             27426..27998
                     /gene="folE"
                     /locus_tag="PC460_000986"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF2599883.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            28062..28853
                     /gene="folP"
                     /locus_tag="PC460_000987"
     CDS             28062..28853
                     /gene="folP"
                     /locus_tag="PC460_000987"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2599884.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28864..29229
                     /gene="folB"
                     /locus_tag="PC460_000988"
     CDS             28864..29229
                     /gene="folB"
                     /locus_tag="PC460_000988"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF2599885.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            29222..29728
                     /gene="folK"
                     /locus_tag="PC460_000989"
     CDS             29222..29728
                     /gene="folK"
                     /locus_tag="PC460_000989"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF2599886.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29709..30494
                     /gene="aroF"
                     /locus_tag="PC460_000990"
     CDS             29709..30494
                     /gene="aroF"
                     /locus_tag="PC460_000990"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2599887.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30749..32539
                     /locus_tag="PC460_000991"
     CDS             30749..32539
                     /locus_tag="PC460_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2599888.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32557..33723
                     /gene="rpoD"
                     /locus_tag="PC460_000992"
     CDS             32557..33723
                     /gene="rpoD"
                     /locus_tag="PC460_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF2599889.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            33888..34577
                     /locus_tag="PC460_000993"
     CDS             33888..34577
                     /locus_tag="PC460_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2599890.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            34591..35688
                     /locus_tag="PC460_000994"
     CDS             34591..35688
                     /locus_tag="PC460_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2599891.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35751..36323
                     /locus_tag="PC460_000995"
     CDS             35751..36323
                     /locus_tag="PC460_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2599892.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36383..36850)
                     /locus_tag="PC460_000996"
     CDS             complement(36383..36850)
                     /locus_tag="PC460_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2599893.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37236..37712)
                     /locus_tag="PC460_000997"
     CDS             complement(37236..37712)
                     /locus_tag="PC460_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2599894.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37832..38923)
                     /locus_tag="PC460_000998"
     CDS             complement(37832..38923)
                     /locus_tag="PC460_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF2599895.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(38916..39305)
                     /locus_tag="PC460_000999"
     CDS             complement(38916..39305)
                     /locus_tag="PC460_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2599896.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39497..39943
                     /locus_tag="PC460_001000"
     CDS             39497..39943
                     /locus_tag="PC460_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599897.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            40207..40932
                     /locus_tag="PC460_001001"
     CDS             40207..40932
                     /locus_tag="PC460_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2599898.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            40919..42163
                     /locus_tag="PC460_001002"
     CDS             40919..42163
                     /locus_tag="PC460_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2599899.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGQGTEFHIIFHKMYDSD"
     gene            42243..42920
                     /locus_tag="PC460_001003"
     CDS             42243..42920
                     /locus_tag="PC460_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599900.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            42921..45482
                     /locus_tag="PC460_001004"
     CDS             42921..45482
                     /locus_tag="PC460_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2599901.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            45703..46977
                     /locus_tag="PC460_001005"
     CDS             45703..46977
                     /locus_tag="PC460_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2599902.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(47138..50179)
                     /locus_tag="PC460_001006"
     CDS             complement(47138..50179)
                     /locus_tag="PC460_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF2599903.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            50386..50685
                     /locus_tag="PC460_001007"
     CDS             50386..50685
                     /locus_tag="PC460_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF2599904.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(50923..53151)
                     /locus_tag="PC460_001008"
     CDS             complement(50923..53151)
                     /locus_tag="PC460_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF2599905.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT        21169 a         5742 c         8736 g        17598 t
ORIGIN      
        1 atgttgattt aaaataaaaa taagatttcg gaggagttta ttatataaat gaaagattac
       61 tataaaatag gagagatatc taaaatatat ggaataggta gagattcatt aatgtattat
      121 gaagaaatag gcattctaag accagttaga gatataaatg gatatagaat gtataatata
      181 tcagatatat ggaaattaaa tttaataaaa gaatttagaa gcttgaactt tccaatgaag
      241 aaaataaaag aatacctaga tgacagaagt atagagtcta cgaaaaatat tttaaatgaa
      301 gaactcgatt taattgataa aaaaatagct gaatttataa gccataagga aaatattata
      361 aaaagattga gttcaattga aagtgtaatt caaaatacaa aaatagatga gatagaggtt
      421 gtatatatag aaaaaagaaa agctttagaa ttaaatgctg atataaagag ggatgaagac
      481 ttcgattttt taattcaaaa acttcagaaa gaatatgaag atagatttaa tatacttgga
      541 aacaacaata ttggttctgc cttttctact gaagcaatta ataaaggtat atttaatgag
      601 tttaaatctg tattttgttt tctggaaagt aatgagaaag tatataatat agtttttgat
      661 gaaggatatt atgtaacttt aaattatact gggagtaatt ctaataataa gatatatatg
      721 gagaaggtgt ttaaatttat agaagaaaat aattataaga taattgctga ccctatagaa
      781 atctataaga tagatataca tgagactgga attgtagagg agtttgtaac ggaaattcaa
      841 gttcctataa ctaaatagct atatttcaat ggattagatg gtaaatagaa tgaaaaaatg
      901 ttgatttgcc actgtgttat aaaatcggta gcaaattata ttttaaatta taagatagca
      961 tgaaaaaata attgtttagg gggttataaa tgtgaaatgt tctaattgtg gaagtgtaaa
     1021 tattgaaaag ggaattttaa caactggtgg catatatgct gaaactattg gattaaaata
     1081 tagaggagat ggaaaattag ataaagttat aaataaaact tttccagtac aatctactat
     1141 atattcagat ttatgtttag attgtggaga aatagttaga acctatataa aggataatac
     1201 tgataggaat tggtgtaaag ataagaaata gaataatttt tgaagtatat taatatttga
     1261 agttgaattt tattaaagat acaagtgaaa aagtataaat gggaagaaat tgttatatgg
     1321 aagaaaactt tgcatatagg ggagattagt tttttaatat aataacattt ttaaatgtag
     1381 ataaaactaa aaagggttct ctagaaaaca atttctattt aattatactt acttaccaat
     1441 accatcactt gatgtaaaca agattctact acattttctc cgtactctag tataaaatca
     1501 tctagctgtg tatctataaa atcacaataa tcatataaac tactgatata agcagttaac
     1561 atgtgtgtac atttatccat ttcatctcta aacatatata ttactatccc cctaaaaaac
     1621 tttcataaaa caaaaagaac aatatctttt tggcaactgg ctgacataac tcatagttta
     1681 ttttataagc gttagtccct atagctttgc gtcactaaat ttctctagtt ttgccgattt
     1741 agttttattc tacaactaaa ataatacact aggataagtt attgttcaac atacttgtca
     1801 gaaaattgaa atatttaaca gtatcttaac aatatgaaat ctatttttca ctcaagaaat
     1861 aaactataca tgtagaatat ttaataaaaa ttatatttag ttttgtattg aagtttttgt
     1921 attaaaaagt ttagttcaca agagttactt ggctagataa tagatgaaca aaaatatctt
     1981 gaaaaaatat atatgaatgg caaaaaacaa gggatgaatt ttatatagac tcatcccgta
     2041 attatttata tattgacctt gattaaatat attattattt ttaagcttca atttttatat
     2101 tatatttcta aagtttctat aagaggatta tcgctaaata attttaataa atttgttttt
     2161 ttatatcctt taggtaactt agtatatata actacttcta accaatcccc atcaaggcgt
     2221 ttaatattca tgtttataat ttcaatatta gattttttaa atttaccttc aatatattct
     2281 atggaatcag tgctgttaga aattttaaga gaaacttgct ctgctgtagg gagtttcatc
     2341 catttcctat ctctatgaag tgttatctgt acaataacaa taatgatact tgaaacaata
     2401 ccaactaagt ataatccaga gccaatggca agacctgtac cagctgttgc ccatatacca
     2461 gcagctgtag taagaccact tactgattga tttctaataa atattaatcc agcacctaaa
     2521 aaacctatac cactgacaac ttgtgctgca acgcgggctg ggtctagacc aatacctgga
     2581 gtacccacta tatcattaaa tccatacttg gaaacaatga ttataagtgc tgaagctaat
     2641 gccactataa aatgagtgcg tattccagct tcttttagtc tattctttct ctcatatcca
     2701 attaaagccc cacagaaacc agcagtaatg attcttgcta tatattcaaa ttgaatcaat
     2761 atatgatggt tcatttatct agcaccccct tctatataat attatatata ctataatact
     2821 acatttttta tgtgaatata gtaaagttta aaatttatat aatagttata tatgaatata
     2881 aaatgtataa acgtatatta tgtatataaa aatgatagaa agcatctaat tttaaatgat
     2941 taaagagcaa gcttaaataa atcaaacttg ctcttttaac atagtgtatg tttttatttg
     3001 tgatagttag ataaaaattt atctgtgttt tttacatctg caaaactgtt ctgtagtgat
     3061 gccatgaata cttgatgtac agtatttgca ttaatttcta tattattcca agataaattc
     3121 ttcgttgtac atgcatcgct tattagagtt atgtcgtatc gaagtttctt tgctgttctt
     3181 acacttgtat caatacacat atggctcatc attccacaga ttactagatt agtaatattt
     3241 tttttctcaa ggatactttg caaatctgtt tcaaagaaac tatctggtgt gtgtttagtt
     3301 ataatttctt cattatctag tggaaaaaca attttattta tttctgaacc atgagtatta
     3361 ggaagaaaaa aagaagctgt tttatctgtt gatatatgct gtatatgaat aactggtaaa
     3421 ttgttttttc taaaaaacag tagtattttt ttagcatttt cagcagtttc ttctgattga
     3481 aataattcac attttccatt tttaaaataa tcattttgaa tatcaatcaa tattaaagct
     3541 atagacatga ttatacctcc tatttatttt tattataata ctattgtaaa aaatgtaaat
     3601 tacttacttt ttggtatgta taggattact tattgtagca aattccttta ttatctagaa
     3661 tttcttgttg accatgttca agcatatatt cgattcctat ttcttgcatt atttccactg
     3721 cttttaacat ttttttacca cgattagtaa gaaaatattc aacatgtaat ggatatccag
     3781 cagaacttat tttatcaata atcccaaaat cacgtaactc ttttaattgt tgaatcaaca
     3841 ttttttgatt tattccataa atattatgtt gcaattcaga aaatgtttta ttagattgtc
     3901 ttaattcaaa tataaggata gttttccatt ttcctttgat tatgtcatgt acaatttcaa
     3961 gaagacatgt atattcagct cttattttca tagcatatca cccccagtaa aatgataata
     4021 aattatatca cttgtcttgt gatatgacaa atatatatga actgtatata aattttatgt
     4081 tttattaagt tctaaatttc ttctattgta gttcatattt agtacataaa taattagtaa
     4141 gatataagta aataaatata taacatgact atagaaattt aactttaaaa taacttatga
     4201 taatttagag gaggaatata tatgaatata tcaagaaaag ctatgaaaat aatagaatta
     4261 gcacaaaaaa ttgcaaataa gagaggtatt tcagtagaag aagcttggag tgaagcagta
     4321 acagaatata aaaataaata cgaacatatt gcatagatat ttaaaaattt agaatatttg
     4381 gtagaatata atgattttaa atgatactat aaattataag gtatatcatt attaacatag
     4441 aaggaggata tctgcttgat tgtaaaaata gataatactt tagaaaaaga attttggcaa
     4501 tatgtatcac atgaagaaag tttaaattta ttcataattg gatacgttga gaattatgga
     4561 tttagctctc aatatcaaga tatatggtct caggtagaag atggaaatat aacctctata
     4621 atattaaaaa ataagtcaac acttataatt tatagtttta aaaataattt taatatagga
     4681 gaaatgaaga atcatataaa agatttagat gtagaaagca taagtggaaa aaaatgtgta
     4741 atagatagac taataagtaa atacaaagat ttttatgaaa aactagataa taaattttgt
     4801 gtattaaaag aaataaaaga aatagatttt tccaacatga aggagtataa aatagaaaat
     4861 gctcaggaaa aagatattga tgaaatagga aaacttttaa ataggtcaga ctataaagta
     4921 agtaaaaatt acatagaaga aagaaaagtt catttaaaag agggaaatgt aagagcatat
     4981 tttattagaa atgatgatac tatgattagc actgtgtcaa ctggtatgga aactagtttt
     5041 ttggcaatgg tggtctctgt aagtacagat aaaagatata ggggaaaagg acttgcaagt
     5101 tatatggttt acaatttgag taaagaattg ttgttagaag gaaaagttcc atgtcttttt
     5161 tataataatg atgtagcagg taaaatatat cataatatag gatacaaaga gataaatgaa
     5221 tggacaattc tttttaaata gctataaaaa ataaaaaact ctatcgagaa ttaaagtatt
     5281 tataaataac tatattttta ctataattca tgatagagtt tttttaaatt tatgatacta
     5341 ttttttatta tttaaataat caagtgtttt aagtttataa ccttcttttt cccaatgtgt
     5401 aagaacctca tctaatatct cagtatttgt tgatgaattt ggatgcagta aaactattgc
     5461 accaggatga gttcttgaat aaattttgtt tttggcaaat tcatgagtag gttgtttctt
     5521 ttcataccaa tctacataag caaaactcca aaatattgat ttatatccta aatcttgtgt
     5581 atattttaaa gattgttcgc taaatttacc cattggaggt ctaaaatact taggcatttc
     5641 ttttccagta acatctttgt atgctttttc tacacttgta atttcttctt tgaatttttc
     5701 aaaatctgta atacctgcca ttgatggatg gctttttgaa tgattacaaa ctaaatgtcc
     5761 ttctttttcc atacgcttta ttaactctgg attgcttttt atataacttt caactacgaa
     5821 aaattgagct ttagcattgt gttttttaag cgtatctaat aattttggag tgtttccact
     5881 ttcatatcca gcatcaaatg atagatatat aactttttca ttgggatttc ctacataata
     5941 agctccgtat tttttaaaaa aatcagcttc ttttataggt gatggctgtt taccatcctc
     6001 cctagggtta aaataccaat catattcatg tgtgtcgagt gttggactag aaatttgagt
     6061 ttttgtttta tctaatgatt taaaatttat actagcaatt ccaaaaagta ttagagcaaa
     6121 tgctcctatc ataatgtatt tttttaggct atcttttttc acaatttcac ctcttattta
     6181 aaatttaaag tatatttctt ataatatagt ttgaatattt atagttaaaa atattcacat
     6241 atacaaataa taaatgaaag aagtttactg taagatataa agttgatatt aattatctta
     6301 aataaagtgt atataaatga taaaatataa agagaaatga ttgtaaataa gttaggctgg
     6361 tttaacttga agggatataa aaatggataa tataataagt gtattgaatg atgttgtttt
     6421 tttagatata gaagttagtg gattagattg tttaaattca gaaattttag aagttggagc
     6481 tgtcaaggtt aaagactgga aaatatatac ctatgaatca ttaataaaaa acaaatttga
     6541 ggtacctgta gaagtattct cagtatgtaa aaatttagat aaaaatgatt tagaaatagc
     6601 taatgaaata gaactggtgg aagatagact tgtaaatttt gtagaagata gtttcattat
     6661 atgtcatgat ttaagtctga aaaaaaagtt tcttgagtat catatgccaa agttaaaaaa
     6721 caaatttatt gatttaattg aattagcagt tattttagaa ccatatcata aagattatag
     6781 cttagaatac ttaaaaaata ctttaacaaa ttgtaattct aaagtggaaa atagagcttt
     6841 atcagatgct attgatataa taaatattgt aaattgcctt ttagtaaaat ttaataacta
     6901 tgaaaaaact actttggagc cattgagttt taaaataaat tcatatctta aaaaatttaa
     6961 tcttccaaca tgggaatgga gcaaattttt agaagaggca aattatgatt tatcaaataa
     7021 tataaatata aaaaaagaat ataatatatt tgactcaaaa gaagaaaaga agaaggaaag
     7081 agaaacctta aaaattttaa atgaagaaga aaaaaattat gaagaattat taaaatacaa
     7141 aactatttgg gagaataaag aaggatttac atatgaatat agacctggtc aatatgagct
     7201 tactaaaaca ataagagagt tgtttagaaa tagcgaagat gaagaaaaaa ttgcatgtat
     7261 agaggcacca actggtatag gtaaaagtgt aggatattta ttgcctgcaa tattagaggc
     7321 tagaattaat aaaaaaagat tattaatttc tactgacaca aaggaattgc agatacaatt
     7381 aataaataaa gatattccaa atgtgttaga ttctttgggt ctaaatggaa aagtaagtta
     7441 cggatatata aagggaaaaa ataactatat ttgtatagat agactggaag catatataga
     7501 tgattatgag tctcaaaacc ctactaaagg agaaatttta tctttaatat ttttgaagag
     7561 attagttgaa ggtggcaagt atggagatat agaagaaata aattatttag tgtttgacaa
     7621 cttcaaggaa ataagtactc acttaagaaa tgtaagttgt gacccaaata tgtgcagacc
     7681 taaaaaatgc aaaaaagatt gtttatataa aaacagaata gaagaattaa aagaagaaca
     7741 tataacagta gtaaatcatt cactattagc taaatggcca tataaggatg aaaaaccttt
     7801 agaaaatata atagtagatg aagcacataa tttgactgaa aaaggatatg atttcttttc
     7861 aagcattata aattcaaagt ctttaaggta tttattacaa gaaatttatc cttacgagtt
     7921 tattcaaaat agtagcttta tatataaaaa atatagtaga aatatgagaa aaataaaggc
     7981 ttttgataaa ttttataatg tattaaaaat tggaagagaa gataaacaaa aaattgctag
     8041 aagtataaat ctcattattg aagagataga ttcaatatta aagtttggta attgtaatga
     8101 atataataat gtaagtaatt ataatttaag atgggaactg aatttgcaaa tagacgaaat
     8161 tgtgggaaaa ctaaaaaagg atggtataga tactgaaata agttatagag cctatagtga
     8221 aaagataaag ttgagttgtg agaaaataat aaaaaattta gtttcaataa taataataat
     8281 ttataggaat attgatgatg atagtataga taaagaagca gatatatata aatttggaaa
     8341 ggcaaagaca agagatttag aggatataaa gattatattt gaaatctttt tagagtatga
     8401 tgaaaaggat gattatgcaa ggatagttga aattgataag aattataatg actttgaatt
     8461 tcgagtagtg ccattaaaaa tagcagattt atttgaagaa aatattttat ctcaattaga
     8521 aaaaggaata tttttatcag caactttgag tttatctgaa agtatgtctt attttaaaaa
     8581 tacattagga atagacagag ttaagaatgt ggaaaagatt atagaaccaa tttttgatta
     8641 taaaaataga gtctctgtag ttggattttc tgatatttgt gagtacagaa attctgaatt
     8701 tccaaatgag atgagtaaaa ttatatctaa tatatcaaag attacagaag gtcatacatt
     8761 agctcttttt aatagtaaag atagacaaga aaaaacttat gaaatattga aaaaatactt
     8821 acatagtttt aacttagaaa tttatgctga taaaaagggg attagacacc ttaacgattt
     8881 aaatagaaaa tgtgtagtac ttggctcaaa aggatgtttt gaaggcgtag acattccagg
     8941 ggatggtttg gtttgtgtaa ctttagacaa gcttccgaat ctaaatccaa aagacccttt
     9001 gtattttact ataatgaaga agtatggtat agactactat actataaatt atcctcaaat
     9061 gaccataaag gtaaagcaag ctatggggag aattttaaga agcaaatatg actatggatg
     9121 ttttgtgatt tttgatgtag gaactaatat aagtgtgtta aagagattag agaaagattt
     9181 acacgattgt aaaatttcaa aggttaatag caatgagttt tatacttata taaggcgaca
     9241 tttaaataaa tatagaagtt taatactaaa atctgttata tttgatacta taaaagcgtt
     9301 gaatgttgat gctaaaatgg ataacaatga tgttgataag gatattataa aaaaagatat
     9361 aaatgaaaat ataagacaaa gagctgtaaa aggagaagtg taccatattg atattataaa
     9421 aaaagatatg aaagtaaaat attttgatag aaactatctt ataaatttag atatatttat
     9481 gagagaagaa gataaaaatt aagagtggga gtgatatatt tggcaaaaca aaagttttat
     9541 gctgtgaaaa aaggtaaaaa tataggtgta tataatacat gggatgagtg caaaaaacaa
     9601 gtaaatggat tctcaggagc tgaatataag agtttttcta cttttcaaga agctaaagaa
     9661 tatattgatg ggtcagaaaa attgagtttt caagaagata aggagtttat agaagcatat
     9721 gtagatggaa gttatgaaca ttcagtaaaa atgtatggtt cgggtgttgt gattttaaaa
     9781 aataatgaag ttataaaaac ttatagtgaa aaaggaaaag agaaaacttt agtaagtatg
     9841 agaaatgtag ctggagagat agaggcttct aaaattgcaa tgcagtactg tattgacaat
     9901 aatgttcaaa acttgatttt atactttgac tatgaaggga ttgaaaagtg gtgtactgga
     9961 gtttggaaaa caaataaaga aggaactata gcatataaaa atttctatga cagtattaag
    10021 aataagctta atgtaaaatt tacaaaggtt aaagctcatt ctggaaataa gtataatgag
    10081 gaagcagata aattagctaa aaaagctatt ggtgtataat aaaaaaatat ttatacataa
    10141 ttacatggta taagagtata tctaaattcc tactttataa ttaaacaact tgtaattaaa
    10201 caacttgtaa ttaaacaact tataattaaa taaattttga ttaaaaaagc agattgtatt
    10261 atgaaaaaat aagtatctaa tttataaaca attaataata gtattaatta gatacaactt
    10321 atttaatata ataaaatctg ctttttatat aactataaaa ttttcctaca ttctattaga
    10381 attgcccata aataccttca agttcttctt ttaatacttc agggtcattt ataccaatag
    10441 acatggttct agccagtata tttgcttggt cataagttga tgtccatgag aatacaccac
    10501 ctagacaatt tctaagaaca tattgagttt ttaagaatat tgataaagca tcatcatatg
    10561 acattgcaaa atctccatcc ttaactaaat atggaacttg agcagtatta tcaaatctat
    10621 attcatatcc atttttattt atataatccc ttctaagctc atcataagtt cttgttatag
    10681 tagcacctaa tcttccataa aatggtatac caagaaggat tttttcagaa ggcattccag
    10741 cattctcaag atttcttacc attgcatcaa cactatatcc tggcaaagat aagtctgaat
    10801 caaaaagatt tgcttgatgt tttctaccat ttgggcctgt ttcacctgct gtaaaatcat
    10861 aactcataag attaaaataa tctattatag gagctatttt atctatttca gcacttgaat
    10921 ttatatatcc tctatctcct gttccagcta cactaagcca tttatcatct cctataacat
    10981 ctcttatggc agttagtaag agtgtaaagt tttctctatc ttgaggtctt gatgttattc
    11041 cagatgcact acttccagga tattcccagt ctatatctat tccatctaaa gcatattcat
    11101 ttatcatctg attgacctgt cttgcaaaat tatatctaga tgtaggtgtt aatgctgcat
    11161 cagagaatcc ttcagctccc caacctccaa tagctacaat tacttttaag ctaggttttt
    11221 cacctttaag agcaagtaat tgatttaaaa atctaggata aggaataaac aaatttccag
    11281 actggtctat ctcagcaaaa gcaaatataa ctgcatctaa tagttgagca tctacattag
    11341 ttggatttcc aagaacatat tctgctacaa ttggatttat atctggacag tttgcatttt
    11401 caactattgg attgtatggg tctgttattt gtggtctaac taatttttta gaatctttta
    11461 aagttttcat agctattttt ttagtatttt tttcgctagt ataatcgtag tttttataat
    11521 tatgtttata gaaatcttca taactactat cttctttgtt atagctagaa tttattttgt
    11581 cacaagattc atctttatca tagttaaaat tcatttcatc acaagactta tctttaaact
    11641 tatcttgttt ggagttgtga actttgttag tatttttagt atgttttttc tcatgattgc
    11701 aatcatcttt aatatcagtc ttgtgttctt gtttacagtc aagagctttg ttcaggtagt
    11761 catgatgttc atgttcacag ttacattcat tttcaatgtt tttagtatgt tcttttttcc
    11821 agctacaagt attatcaatt ttcttgctca cttcttcatc attataatta tctggtacaa
    11881 aacataagta ccagtccata catgaatatt tcttattaca actgttaact ctatttttta
    11941 attccttata gtttttagga taaggtaaaa tcactggcat tccaggaaac cagtttgcag
    12001 gagtaactat attatctcta tcactagtct gaagtgcatc tactattcta agtatttctg
    12061 gaatatttct tcctgtagtt agtggataat aaagaatcgt tcttagaatt tgattattat
    12121 ctataataaa tacagagcga acagttgatg tatcactcat tggttttgat atcatgccgt
    12181 ataacttggc aattctcata tctctatctt ctataatagg aaatggaatt tctacacctg
    12241 taagtaaaga aatattatac atccaagcta aatgtgaact gttactatca acacttagac
    12301 caattagttc tgtatttctt tttttaaatt cgtcataata tttagcaaaa cataaaaatt
    12361 ctgtagtaca aactggtgta aaatcaccag gatgtgaaaa taaaacaatc caattaccct
    12421 tatagtcaga gagtctaata ggaccatttg ttgtattggc tttaaaatca ggagcctttg
    12481 accctaaact tggcaaattt ggcatgtaaa tcacccctca ataaattatc atttatttta
    12541 ttctattaat taatacatat tagtgttact attttattaa aagttaagta atccttatgt
    12601 caaaaaaaga tatttattaa aaagatttaa tattttccta atataaatga caatagtaat
    12661 tgctattttt ttactaattt ggtatagtaa tggataaggg gtagtgtcaa ttttagtgat
    12721 tatacgaggt gagtggattg aaaaaaattc taagaatttt agttatctcc atgatgatat
    12781 gttttgcaat gacttttgca ttagtgttaa taaattcagt tatgggagta catataggat
    12841 tggattatta ctttttgatg ggaagtgtgc ttacagtttg tttaacaatt ggaattgttt
    12901 tatgcaatgc tagaaaaata tttgatagca aagctataca caagacaaga acgaaaactg
    12961 tcagtaataa taaaaaacaa aagagtcatt ctagggatat aaaaagaaag atatcataat
    13021 ttatgtaaag gaaatattta tgtataaaat aatgattgat aggtaaaata aaaacacgat
    13081 ttaacttgtg tttttatttt ttttcaagaa ttatgatata ataaccaata agaaatttag
    13141 tttaccataa aatataaaga ttattaaatg gagagggaac tataatgaat tttagagatt
    13201 tatttgagaa ggctgtagat tttattgatg aaaaaaggaa atctatagtt gcgatttcat
    13261 tatcaacatt aggtgtaatt gctttgataa tagtattttt cttaagtagt aacgagttta
    13321 gtgtaggaaa tgaagctaat gagttgttaa agattataga gaaaagacag tattctatag
    13381 ctgttgatta ctacacaagt atagaaaaaa agttttctga ttcaaaaatg gaaaggttta
    13441 ataagtctgt ttctaaaaaa ataaataaat tgctattaaa tagtggtgat aaatatttag
    13501 atggagacat aagtcaagaa agttttattg gattaataaa taccgtgaaa gaattaaaca
    13561 aaataagtat agatgtggat gacttattag ctcaagctga tagagttaga gaaatgtata
    13621 aagaagaaaa tactacttat gatattgcca ttaactacat aagtactgtt tcaatattaa
    13681 atggtatggg tagtaaccta gatgtataca aacagaatat agaaacaatc aaagagtcta
    13741 gagatgttta tgactcagca gtaaaagacc aaaaagcata taagtattat gaagctattg
    13801 aaaagtataa taaagtatta aaagaagatg aaaagtatta cagtatggct caaaatggga
    13861 aagaacaatg tattgaagaa atgtatgatt actatataag tagagctgaa gaggcaaata
    13921 cttctggaga ttatgaaaga gcattacaat atattgagta tttaaaagac gattattcag
    13981 atgatgaaaa ggtacagagt ttagaaaaaa aatataaaaa gaatttatca ttgtatactt
    14041 taacatctga tgacattata aatgtaatag ctaaaaaaag tggcaaagac aaggcaaatc
    14101 tatctataaa ttcacttcca caaatgataa aaaataataa atactattat gctgaagtat
    14161 atgagtatga taaattaata aatgaagttt tggtaagtgc aaaaactaaa gatttatatt
    14221 catataaaga tggtaaaaaa gattacaaag tagattatgg tgatggatac tttagaatat
    14281 tagaagatgg aagttatcaa tttggtatta caaaagataa agctaagttt gtgttaacta
    14341 atactcttga tgaaaaagaa aataagtata aaaagattga gatattagat atagagaaag
    14401 cagatagata tgtaaaaagt aaaaagagtt tagaagaatt gtttggaaaa tataaaaata
    14461 tttattatta tgctgtagta aataaaggct tatttagagg caaagaagtt tatgctatta
    14521 atatttacaa tgaaaaaata tatgccatat cagaaaatgg actaaatgaa tattaattaa
    14581 taatattaaa ttaatatata attaaaaagt ttttggggtt ttaaggcgca ttatgtagta
    14641 atataataat agggatatat aaataatttt attgtaagga gaagtatggg atgagtaatg
    14701 tgaacaaaaa gctagttata gctattggag ctgtagcatt agtagtaata atttttatag
    14761 gcataatgtg catgcaattt aaaggggaga aaatagcaaa aaacacatat gttaatggag
    14821 taaatattgg aaaattaact aaatcacaag caaaacagga attagctaaa aaatacaaat
    14881 tagagaatgt tgattttaat tacaatgata aaagctggaa agtaaagagc aaagatttaa
    14941 atttatctac tgacctggat aaaacagtag aaaacgcata taatttaaat agaaagagtg
    15001 ctttctttgg caatctatca aaaactattt cagcaaattt tggcaaaaaa agcaacttag
    15061 tagttgcgat aaattatgat aaaaataaat taaaagcaga aatggaaaag atagctaaag
    15121 aaatagatgt agatgtaaaa gatgctacac ttgatataag tggtgaaaaa gtcaaggtta
    15181 taccagattc tgatggtctt aaaatggata tttctaagag tatggagaat tttgataatc
    15241 aaactaaaaa aggtaactat aaaaatgagt tagtagtaaa agctactcca gcaaaagtaa
    15301 aaaaagagca acttgctaat atagatacaa atttaggtac gtattcaaca acatttaaaa
    15361 cttctcaaat aaatagaagt ataaacataa aattagcaac agataatata agtaatgtat
    15421 tacttatgcc aggagaaact ttctcattta ataaacatac agggaaaaga agtaaggaaa
    15481 atggatataa aagtgcacct gtaataatgg aaggtgagat ggaagaagat tatggtggag
    15541 gagtttgtca agtttcatct acgttataca attctgtgct atacgcaggt ttagaaattg
    15601 ttaatgtaaa aaatcatact ataccatcaa gttatgttcc aaaaggaaga gatgcaactg
    15661 ttgcagatag tggaatagat ttcttattta aaaataatct aaagcatcca gtttacataa
    15721 aaaattacgt ttcaggaaat caaattgttt gcaatatata tggaagtgca gaagataaac
    15781 aaaatataac aatatcaaca aaattagatg gcgtttcaca aactacaatg aaaagagtta
    15841 atgaccctac aatgcctaaa ggtaaagaaa aagtagacaa aagtggaaga aatgcatatt
    15901 ctgtatcaac atatagaaca tttaatgatg caaatggtaa aaaaattaaa actgaaaaaa
    15961 tagctaattc atattatcct aagaaggaag gtattatatt agttgggact atggaaccaa
    16021 aaccagagga aaaaccaaat acagacgaaa ataagaataa tcaaaataca aacaatcaaa
    16081 acccaaataa tcaacaaaaa ccagaaacac caccaactga taataagcct aatgagacac
    16141 aaccacaacc gcaagcttaa agttctcaag aataggattt atgtagagta ataaaaaata
    16201 taaatctaaa taggagggat tactttgaat ttaaataata taacgaatta cgttcaaagt
    16261 atttgcgaga atatttctaa tgtcttggat gtagatgtta ctttagttac aaaagattta
    16321 actaggatag caggtacagg aatattcaga ggtaaaatag gagagacaat atcagagaag
    16381 tcagtgtact ctcttgtttt aaaagatggg aaatcctatg ttataaataa agaaatagaa
    16441 gagaactgta ataaatgtgc aaatagagaa aattgtaaag agttagctga tatatgtact
    16501 cccgttaaaa tgggagagca tatacttgga atcttgggta tagctgcgtt taatgaagaa
    16561 caaaaagaaa agatactttc aaaagataag gagttaactg cttttgtaag gagtatgtct
    16621 gatttaatat cttttaaact agatgaactt tataaacaag aagttaagac tatagatgac
    16681 cttgagaggg aagctataga aaatgccata aagaaatatg gaagtaatac agaaggtatg
    16741 aaaaaagtag ctgaggcatt aaatataggt atagctacac tctatagaaa agtaaaaaaa
    16801 ttcaacatta aaagttaata gtattgaaaa agaggtaacc tttttatgaa aagaagagga
    16861 ttaccttttt tattatgaga atggtggaaa aatatgtata gttttgactt gacatattaa
    16921 atatgttatc ttaaatatat attttagagt ggaggtgagt attattttag ttaaagatat
    16981 aaaacaagac atagaaagca tgcctagaaa aattttagat atatctttga tagaagataa
    17041 taaaagtgat ttgacttttt ttaatataga agaagaagac ggtgatgaaa cattaaaagc
    17101 attaaaacag agttatgtga ctacaaatta taaagaagaa actctattaa acttgcaagg
    17161 agctttaaat tcaaagtcag atacaacgtc taaggagtta aaattagaat catataaaag
    17221 atataacaat gcattggatt tagcatataa aaattatata actagtgcca ttaatatggt
    17281 taataaagct ctggagataa atcctaaaga cattgatata ctaaatctta gaggtctttt
    17341 aaaattatta aaatgtgatt ttgctaaatc atttgagagt ttttatactg ctttatgtta
    17401 tgaaaataat catatatcta gaaaatatgt aaatctaatt tcatctgaag attttaaaac
    17461 ttttttagga agatacaatc attctataag atttataaat gaagaattaa attatgagtc
    17521 aatacatatt ttagagaaca taattgaaga agaacctgaa ctaatagagc catatgtaat
    17581 tttatctttg ttatatgata aactaggtaa tgtaaaaaag agagaaggat atttagacaa
    17641 acttaaagag ctagataaag ataatcctgt atttgaaaaa aatgaagaag aaaaagaaga
    17701 tacaagtaaa aaggaagaaa taaaaaaagt aaaaagaaag aaaagtattt tgccatatat
    17761 tatagttggc tgtgttttaa taggtatggg gatatatttg attcaaagca aaaagagaat
    17821 agaaaacttg aataaccaaa taagtagtaa agaggaaaag ttaagtgaaa ctgataaaaa
    17881 gttaggagaa acgagtaaaa aattagaaaa aacgaataag gaattaaatg aggttaaaaa
    17941 taaagaacca gaaagagaga taactgtagc tgatgaggaa gatttatatt atcaagctct
    18001 aaatttaaag aaaaacaaag aatatgaaaa agccatagat aattttaaaa gtgtagtttc
    18061 ttctggtaaa actaaaaaat atatttctga gtcaatatat caattagcaa taacaaataa
    18121 attattagga aataaggatg aagctataaa atattacaaa aaatatataa atacttatac
    18181 taaaaatgac cagtattatg atgattcata ttacgagttg ggtatgtttt attatgataa
    18241 tggcgattta aaaaatgcac aacagacttt ttatagtttg agaagtgaag ttccagacag
    18301 tatgtataac aattcaaaaa taaaagaaat tttaagtgaa aaataggttt atatagttaa
    18361 gttttttgaa acaaccaagt atgtatttta ttaaattaaa ataaaaataa atttaaaaag
    18421 agattgtgtc aaattataac tttctatttt aaagaaagat atttgcttgt tgataaaact
    18481 acaaatatat gatttgaagc aatctcttta atttttggag gtggaaaatg aaagttttca
    18541 atcaattggt aataatttta tctatctggg taataggaga gtatataagt tcctttatac
    18601 aaggaattat acttattcct gggagtatag ttggtatgct attattattt ctactactac
    18661 aatttaaagt tttagacttg agtagtatag aaaatgtaag tggatttttc ctcgataata
    18721 tggctatatt ttttattcca gcaggtgtat cacttataaa gtcattagac ctaataaggg
    18781 aaaatgtatt agtgctttta ttagtcatat gtttaagtac actaatagta atgtatacta
    18841 ctggaattat tgtggagaaa atgataaaaa agaaggaaga aggacaaaaa aatgtataat
    18901 gtattacaga caccagtatt tggtataata gtaacaatag tttttttcaa tttaggaaga
    18961 tacattcaac gaaaaacaag taatcctata tgtaaccctt tattaattgc aatcgtagga
    19021 ataatattgt ttttaagcat atctaaaatc ccttatgaga attacaagat aggagctgat
    19081 agtctaaatt tctttttagg tcctgtcaca gtagtacttg cagttccact ttataaacaa
    19141 tttgaattat ttaaaaagca tatgtttgag attattgtag gaataggttg tggtatagtg
    19201 atttcttttg tatctgtatt gataattggc aaaatagcta attcagatgt aagtataata
    19261 aattcactaa ttccaaaatc tataacaaca ccaatgggaa tatctttagc aaattcttta
    19321 aatggtgtag aatcaataac agttgtagcc ataattttta caggtatttt tggtggtatg
    19381 gttgcttcta cagtattcaa actggggaat ataaatcatc cagttgctaa aggaattgct
    19441 cttggaacat ctgcacatgc tttaggaact acaaaggctt ttgaacttgg agaaatagaa
    19501 ggagctatga gtggtgtttc tattggagtc tctggtacaa ttacagtaat actaattcca
    19561 attataatga attttatata aaaaataaaa ttaagatgag taccttgaat acttaaaaca
    19621 aaataaaata aaaaaaattt aaaataaaaa ctgaacgaaa aaaatgcaaa taatgaaata
    19681 aaaaaatcat gtactgaaat tataaaaaga aaaatttcat ttaggaatta attataaaga
    19741 gacaattact aaacttgaat ttgctaaaat aaaataaatt actgctaaaa tagatattta
    19801 gaaaaaaata ctataaattt actatattgc aagttaaata ataagcagta tcatattatg
    19861 ctagaaaaag tttcatttaa ggcataaatg agatgatttg aacgttttta aagattagaa
    19921 tataaaagat tgcactagac atatatgaaa aagataatat aatagatgat gtaatagatg
    19981 acagttcaag aaattataaa aaaaatttat ttgaaatatt atctattaca tcaggaataa
    20041 tatatgtaag agtaagcttt taaaattcta aagatttaga aagattgaag ggagaaagaa
    20101 atgagtgaag ttaagaatac cctacaaaaa catccagttt taagatattc aacaatgcta
    20161 tttgggatgt tgatgacatc tataggaata aatgggttct taagaccagc acatctgctt
    20221 agtggaggtg ctacaggtat agcaacatct ataaactatt tgacaaatat aaatgtaggt
    20281 ttattaacat ttttaatcaa tattccaata tttatcttag ggttcatata ccttgaaaaa
    20341 gagttttgta tttcaagttt ggtaaatatg attgtatttt cattattatt aggagcaact
    20401 caggaaataa gtaatattat acctatacat gatatattgc ttcaaagtgt atatggtggt
    20461 atattatctg gtttaggagt cggagttgta tttagaacta ggtcttctca aggtggtaca
    20521 gatataatag ctgcaatact aaaaattaag aagaatatag aaatgaaaga cactgcttta
    20581 gcaataaatg gcttgatagt acttacagga agttttttat ttgggttgga tttagcatta
    20641 tacactctta taggtctatt tctaaatgcg tattcaatga gttttattaa agatgcaatg
    20701 aattaccaaa aatctgttat ggtaatgtca aatgaagtcg atttaatagc agaagatata
    20761 atgaagagtt tagttagagg tgtaactttt ttagatgcag aaggtgcata cactcatcaa
    20821 aaaaagaaga ttatatatac aatagtttca tcaaatgaga taccaaagat aaaagatata
    20881 gctttgaagt atgataaaaa ggcctttata tctgtaaatg atgttacaga agtaaaggga
    20941 agagggttta aagcaaaaga tttatagaaa attttacaag ctgggagttt ataatgtaaa
    21001 tttgtatatt atatctctca gcttatttaa atttagtata cgaaaataaa ggttttatta
    21061 atgatttgtg tatcacttta aaagatatca ttttaaaatt aaaattgaaa aaattactta
    21121 atatgtagaa aacatgacca ctattaggat ataataaatt taatagattt agtaaaatac
    21181 ctaggaataa cgttggagga atggcattat gaatttatct aaagaatatg ttaatttgta
    21241 tgataattat ttgaaatata taaatgaagt aaacaaagag attagaagta taaagaacat
    21301 gaagaaacgt gttttaaaaa ggggcgattt tgctccggaa aatgaagtat tagaattgga
    21361 aggaaaaaca atacgcgata tagatgatgt cgatacaaaa aaaccaaaaa ataaaatcta
    21421 taaattttca aatggggatg tatatgtagg gaaattctta gagggaaaga tggaggggaa
    21481 aggctcatat actttttttg tagatgaagg aacagttatg gagtacatag gagaatttaa
    21541 agaagataaa aaaaatggaa aaggaaactt tatattttca aatggaaatg aatatatagg
    21601 ttattttgag gaagatatga tgagcggtat tggaaatatg ttatatagta gtaaagatga
    21661 atatattgga acttggaaaa acggaaaaaa agatggaaaa ggtatttata agtggagtga
    21721 tggatgtata tatgcaggtg aatttaaagg tggaaagatg gaagggtacg gtatttgcta
    21781 taatagtaaa ggggaagtac tttatgaagg agaatggaag aataatttga ttcatggtaa
    21841 gggaacttat atatgggaaa aaggtaaaaa gtatataggt gaatttatgc atggtaaaaa
    21901 acatgggcaa ggaacttttt atctaaataa tgaattggtt tatgaaggga cttggaaatt
    21961 tgataagcca tctatatttg atagaacatt agatgaaata ttttccttga gactgtagct
    22021 ttatctaatt agtagataaa gcttttttct aatacttact gtaaataggt atgaatgata
    22081 ttgattagta tacactatca atattaactt aataaatgca atattaaaaa attttgattg
    22141 attttaagaa ggagagatat attgaagaga ttaattgctt tagtgttagt agtacttatt
    22201 gttttatttt atagtcctga attattaaaa gaaggatatg atttaaaaga aaagtttttt
    22261 caaagtgaaa tatggaatga atttttagac aaaataaata taaatgataa ttctaagaaa
    22321 gataaaaaag aaaaaagtca aaatttacaa tcaaataata ctgaagggga gaacttagga
    22381 gagtcagata taaagaattt tgaaaaaata agcttaggag ataatttaag ctatgtttta
    22441 tcaagtatcg gcaaacctgg tagaattgat attagtgaat atggttttga ttggtatgta
    22501 tataatcaat atggaaaaga atttgcaatg gttggacttg aaaatgatga agtagtagca
    22561 ctttatagta attctattaa ttcatgtgaa aatcaagata taaaactaaa tcaagataga
    22621 caaacagtga gaactaagat tacaccatta aaatataaga gaaaaggaaa taccagatat
    22681 ataataaact cagaaaatca atatgatata ataagtaagg aaggtaagta tattacaatt
    22741 ttctacgata ttcatgaaga aaatagagtt tgttcatatt taataattga taaaagcact
    22801 gaagatgagt ttaaaaattt atatccagat gatagcgaag aacttaaaaa atgttttgaa
    22861 cttgaagtta tagaccttgt aaatagtgtt agaaatcaaa gagggttaaa ctcattgaga
    22921 tacagtgaac aggcaacttt gagttcaaga aaacatagtg aagatatgag agataataat
    22981 ttttttgacc atgtaaataa gaagaatgaa actccatttg acagaatgaa aagagaaggt
    23041 atagtatata cttcagctgg agaaaacata gcagcaggac agattaatgc aatatatgcg
    23101 catgaagctt ggatgaactc tgaaggacat agaaaaaata ttttggggaa ctataataat
    23161 attggagttg gagtaatatt tggaggaagc tataaaacat attacactca aaatttttat
    23221 aaataactaa aatatttgaa aggaagttaa aaaatgacat catggaaaaa aaagacagta
    23281 tataagtgct taatagcagt agccttgttt tgtggaatag tgttaatatc taattttagt
    23341 aaagtttctg ctttgatgat ggatactaat ggaaatgttt tgattaaaca tggttcaagg
    23401 gaaaaaaagc taatagcaat tacctttgat gatggtcctc atcctaaaga aactagtcaa
    23461 gttttagatg tattaaagaa atacaatgta aaagctactt tttttatagc tggaaaacat
    23521 gcaaaatggt ataaagaacc attagtaaga gcaagtaaag aaggtcacga gataggaaat
    23581 catactttca atcatcctga tataagtaat ttaagttcta gtcagataga agaggagatt
    23641 gttaagtgtg aagacatttt gaaggaagta actgggaaaa aacctacatt atttagacca
    23701 ccattcggaa gttatagaga aaaagactta atagaaattg caaaaaagca tgactataaa
    23761 gttgttttat ggacaggtgt agatgtgaaa gattggaaaa atccaggagc taatagcatt
    23821 gcagataaaa taattaataa agtacaaaat ggagatataa tacttttgca tgattatgca
    23881 actaatgata cagttgaagc tttagatatg tttattccaa aaatgattga aaaaggtttt
    23941 aaatttgtaa cagtttctga gttgataaaa taatttttag gggggagatt tacagtatga
    24001 ttcttatgat agacaattat gattcttttg tatacaacct agtccaatat atagaagaat
    24061 taggggaagc agttgtagta aaaagaaata atgaaatcaa aataagtgac attgaagaac
    24121 taaatccaga ggtaattgtg ttatctccag gaccgtgttc tccaaaagaa gctggtattt
    24181 gcatagatat tgttgagcat tttaagggga aaaaacctat actaggaatt tgtttaggtc
    24241 accaaactat tggacatgtt tttggaggag atattataaa agcacaacag cctgtgcatg
    24301 gaaaagtata tagtatcaac catactaata aaggagtttt taggggactt aaaaatcctt
    24361 taaatgtcac taggtatcat tcactaatag ttgactccaa tacagttcca aaagaattag
    24421 aaattacagc aattactgat aaaggggaaa taatgggaat taggcataag aaatatttaa
    24481 tagaaggagt acagtttcat ccagaagcta ttttgtcaga gtatggtcat gaaatgctta
    24541 aaaattttat aacagaagct agagaaaggg tacatatgta atatgataag agagataaat
    24601 actaaattaa attcttttga gatatttact atttttagaa atgaacatga cagttttata
    24661 ttagatagtg ctatggataa agaaaaattg ggaagatatt cttttataag tagccaacca
    24721 tttaaggtat taaagtataa agatactgat gaaaatcctt tagaagtttt aaaagaagaa
    24781 ctacataagt atagggttgt aaatgatact aatcttccat ttgtaggagg tgctgtggga
    24841 tatttatctt atgatttagg taattacata gaaaatttac ctagaactgc tgtagatgat
    24901 attgagatgc ctgatatgta ttttggattt tataatcatg taatagttat agaccatctt
    24961 gtgcaaaaaa cttatatagc tactccaaac atagatatcg aattagaaga aaagataata
    25021 gatgatatag aacaaagaat attaaaagaa gagaaaaaag gtatagatag catatgttat
    25081 gaagaaaaag aagtaacacc tataagactt aaatctaatt ttacaaagga agaatttaaa
    25141 aatgcagttc aaagtgttag agagtacata agacaaggag atatatatca agctaattta
    25201 acacaaaggt tcagtggaga aacagaactt acaagctttg aattgtatag ggacttaaga
    25261 agatttagcc cagcaccatt tggggcattc ttaaactttg aagatgcaca tatattatca
    25321 aattctccag aaagatttat aagatgtgtt aataagagaa tagaaactag accaataaaa
    25381 ggtactcgtc caagagggaa agataaagaa gaagatttaa gacttcaaca agaacttaga
    25441 aatagtgaaa aagatagagc tgaactactt atgatagtag atttagaaag aaatgatatt
    25501 gggagaatat ctaaaactgg aagtgttaaa gttccagagc tatttgtaat tgaaccatat
    25561 gcaaatgtaa atcacttagt ttccacagtt gtaggtgagt taaaagatga taaagatgct
    25621 actgatgtaa taaaagcaac ttttccaggg gggtctataa caggagcacc taagattaga
    25681 gcaatggaaa ttatagacga attagagcca acgcaaagaa atgtatatac tggttcaata
    25741 ggttatatag gttttaatgg tgatatggat tttaatatag caattagaac aataatcaaa
    25801 aatgacaaaa aagtctactt tcaagttgga ggaggaatga cttgggattc tgacccagat
    25861 gaagaatatc aagaaacact tgataaagct aaatccataa tgaaggcttt gaggggatat
    25921 tatgaagaat aatgtaggtt ttgatagtag tttaagtaaa tttggaattg gtttgtttga
    25981 aactattaga gttaaaaatg gaatagcagt agatttagat atccatatag aaagaatgct
    26041 aagctcaata aattgtttgg atttagatat aaactataaa aaagatttct tgataaatga
    26101 aattgttaca tatataaaaa aagaaaatgt cattaataaa gcacttagaa ttactgtttt
    26161 tgatgaaggt tataatatat ctattaggga tattccatat aatcaagaga cgtatgagaa
    26221 ggggtttaaa ttagctatat ctccaatagt tagggggaat agtttaattc atagacataa
    26281 gactactaat tattttgaaa acatatatac caaaaatatc gcaaataaga ctggttataa
    26341 tgatggtata tttttaaatt cagaaggtgt aatattagaa tgttctatga gcaatatttt
    26401 ctttataaaa ggagaaagag tgtacactcc tagtgataga ttaccgatat taaatggaat
    26461 aataaaaaaa aggattatag agatttgtga cgaattacat atagagttga tagaaaatga
    26521 aattaatata tcagaaatta gtagttttga ttttgttttt gtcacaaata gtcttatggg
    26581 cgctataaag gttacagaaa ttgacaaaat aaaatttaat aaagaaaatg ttgtttttga
    26641 taaaatagtg aagttattag aataaattaa aatattaaca atttgttgga gacaaaagat
    26701 gataggttgt atttatgaag tatacaatga agagatgact aaaataatag aaaaccaaaa
    26761 cactttggct gtatatttag tgggttctag taaagatgta gattttactc tttatgatgt
    26821 agtgataaac gatattgatg tgttcgtttt tgtaaaagaa ggggaaaaac aagagagaat
    26881 actgtttaaa aaaaagggaa tagaatttga tgtaaattac ttttcaaaag atggtgtaaa
    26941 aaaattatta tcaaataggg aatatttctt tgtgaaagaa atgaaagatg ctaaagtact
    27001 gtttgatagg gagaatatat cacatattat aaaagatata agtagagata tttacttaga
    27061 aggacctagt aaaatgtcat tagaggaaaa gtcttttata aagcaagata ttggagcaaa
    27121 aatattgaat ttaaaaaata aagaaaaatt tgatgttttt gaatatcatt ttttgacgaa
    27181 tttatattta aaagatataa taattgggta ctttaatata aatgataaat gggtaccaaa
    27241 agacaaaaaa ttgctaaaag ttttaaaaaa agaaaataat gagctttttg aactagtgtc
    27301 aaaagtaagt gaaaattatg actatcaaag acttttagat gtttataatt atatttttaa
    27361 agaaattgag acaaaagagg ttataaaact aatcttctag tcacaaatca atggggggag
    27421 tttaaatgaa taaagttgat aaagaaaaga tacaacatgc agtaagagaa atactagagg
    27481 caattgggga agacccagat agagaaggtc taatcgaaac tccaaataga gttgctagga
    27541 tgtatgaaga gatattctct ggtcttagtg aagaaccaag agaccattta aaagttcttt
    27601 ttgcagatga aaaacatgaa gagttagtat tggtaaaaga tattccattt tattcatgtt
    27661 gtgagcatca tctagttcct ttctttggaa aagctcatat tgcttattta ccaaaaggag
    27721 gaagacttac agggctttct aaacttgcta gagtaataga tacattggca aaaagaccac
    27781 aattacaaga aagaatcact aaaaatgctg cagatataat aatggaagaa ttacaacctt
    27841 atggtgtctt agtagtagta gaagctgaac atatgtgcat gacaatgaga ggcgtaaaaa
    27901 aaccaggttc aaaaactgtt acatcagcag tgagaggaat atttgaaaag gacattgcgt
    27961 ctagagccga ggctatgagt ttgattacta tgaagtagta atttattata gatataatca
    28021 tttattgaga taaaatttaa tttaagggag tgtcattaat tgtgtttgat tatggtaaaa
    28081 gaacttatat aatgggaata ttaaatgtaa ctccagatag tttttctgat ggaggagatt
    28141 ttaataactt ggacatagca atacaacatg caaaagatat ggttgaccaa ggtgcagata
    28201 taatagacct aggaggagag tctacacgcc caggtcatag ctatgtagac tcagacgaag
    28261 agctaagaag agtaatccct gtaataaaaa aacttaaaca ggaattggat ataccaatat
    28321 caatagacac ttataaggca gatgttgcag aagaagcatt aaaattaggt gtcactatgg
    28381 taaatgatgt ttggggtctt agaaaagata aaaatatggc ttctgtaata ggaaaatatg
    28441 atgcagaagt gtgtataatg cataatcaag atgggacaaa ctatgacaaa gatataatgg
    28501 agtcaataaa agactttttt aaggtaagta tagaaatggc aatgagttgt ggtgtaaaaa
    28561 aagaaaaaat agtacttgac ccaggtgtag gatttggcaa ggattttgaa caaaatattg
    28621 aagtattgag aagattaaat gaattaaaag atttagggta tcctattttg cttgggacat
    28681 ccagaaaatc agtaatagga aaagtacttc cagttgagcc taaaaaaaga ttagaaggta
    28741 caatagctac tactgttctt ggtataagag atggcgtaga tatagttaga gtacatgatg
    28801 tttatgaaaa tttaatggca gctagaatga ctgatgcaat atatagaaag taggtataat
    28861 ataatggata aaatattact tagtaatcta ggattctatg gatatcatgg tgttcttaaa
    28921 gaagaaaatt ttctaggtca gaaattcttt gtcgacatgg aactttatat tgattcaaga
    28981 gaagcaggtc ttagtgacga tataaataaa tctgtaagct atgccgaggt ttacaatgtt
    29041 gttaaagata ttactgaaaa taaacaattt aatcttttgg aggctttagc ggaaaatatt
    29101 gctgaagaag tcttgaataa atttatactt ataaatggag ttatggtcag agttagaaag
    29161 cctgaggctc ctgtaaatgg tatttatgac tattttggtg ttgagataag gagaacaaga
    29221 gatgaataag gcttatttgg gaataggtac taacatgggt gatagatttg ataatttatc
    29281 aagagcatgt gaacttttga aaaatagtga ttctatatat aaagtaaaag aatcaagctt
    29341 atacgaaaca aaaccatggg gatatacaga acaagcagat tttctaaata tgtgtgtaga
    29401 aatagaaaca gagtttgaac cttatgagtt gttagaatat tgtcaagaaa tagagagaga
    29461 actacacaga gagagaatag ttcattgggg accaagaact atagatgtag acgtactatt
    29521 ttttaatgat gtagtttcca ctgatgaaag actaacaata cctcatccaa ggattcaaga
    29581 tagagctttc gttttgatac cacttatgga tttaaatgaa gaacttataa taaatgaaaa
    29641 aacaataaaa gaacatttaa acctcctatc tgctgaagaa agagaagaag taaaggagct
    29701 tgtaggttat gaaagaaaac ctatttaata ataattcaaa gatagagata aatacaaata
    29761 acaaaaagat aactgtaaaa aaagataaac ttataatagc tggaccatgt gcaatagaga
    29821 gttatgagca acttttggag actgctaaat ttgtaaaaag tcagggagca aatattttaa
    29881 gaggtggagc atacaagcca agaacatctc caaattcatt tcaaggatta aaaaaagaag
    29941 gtcttgaaat attaaaagcg gttaaagacg aagttggtat ggctgttatt acagaactta
    30001 tggatgtaag agatatggac gaactgtata gtataagtga tataattcaa attgggtcaa
    30061 gaaatatgca aaactttact cttttaagtg aagtgggaaa acaaaataaa cctgttatgt
    30121 taaaaagagg aatagcttcc acaattactg aatggatagg tgcttctgaa tatatagcaa
    30181 tagaaggaaa tagtaatatt ataatgtgtg agagaggtat aagaacgtat aatgattata
    30241 ctagaaatac attagattta gctgcagtac ccattattca aaaggaaaca ggtcttcctg
    30301 ttgtggtaga cccaagccat gccactggag ttagatattt ggtaaaacct atgtcactgg
    30361 catcttttgc atgtggagca gatggaataa tggtagaagt acatcctgac ccagaaaatg
    30421 ctttgtcaga tggtgctcaa tctctttgct ttaatgaatt tgaagattta atgaaatcaa
    30481 ttaataatta ttaaaagagg caagagcctc ttttttattc gtaatcgttg ctaaggtaaa
    30541 tttataagtt ataatttgat agttagatta tttaatataa ataaagattt aatattgata
    30601 gaattaatat aaaaaataaa aattttttga ttaataagaa ggatatcaat aaaaaaagtc
    30661 gaattgtgta tattgagtga ttgacaaatt tagaatatag tgtagaatag aaatgttaac
    30721 acaaaaaatt acaccagaag gtgataatat gaatgatata aaagacatta ttgaagaaat
    30781 aaaagctaga tgcgatatag caagtataat atctgaatat atgaatataa aacaatctgg
    30841 agctaattac aaaggattat gtccatttca tggcgaaaag acaccttctt tttatataaa
    30901 tacgtcaaaa cagatttata agtgttttgg ttgtggtgaa ggtggagacg taataaattt
    30961 tgtcatgaaa atggaaaatt tggattttat ggatgctgta aaaattttag caaataaatg
    31021 cggtatagaa attaacacta acatgaatga agaaactagg attaaaatag aaaaatctaa
    31081 gaaatttcaa gatattcata cggaagcagc tagattttat ctttcaaact tattgggaag
    31141 caaaaatctt gggtatgaat atttaagaat aagaggacta gatgataaaa ttattaagaa
    31201 atttggttta ggattttctt tggattcatg gaattcactt atgaatactc taatcagtaa
    31261 aggatataaa aaacaagact tattggagtg cggtctaatt gcaaaaaata gagatggaac
    31321 taattgctat gataaattta gaaatagagt tatgtttcct atattcgatt atagaggaaa
    31381 tataatagga tttgggggaa gagtattaga cgattctttg ccaaaatact taaattctcc
    31441 agacacgtta atttttaata aaaaacagaa cttatacgga ttaaattttg ctagaaaaaa
    31501 tttagaaagt aaaactatag tcttagttga aggatatatg gatttaattt cgctttatca
    31561 gtatggtata aaaaatgtag tagcaaccct tggaacagcc ttgactgaac aacaaggcat
    31621 tttaataaag agatatgcgg acactgctat tatatcttat gattctgatg aagcaggaat
    31681 aaaagcgact ttaagagcta tagatatact tactaaatta ggtatcaatg taaaagtgtt
    31741 agacttaaaa gatgctaaag acccagatga atttgtaaga aaatacggac ttagtgatta
    31801 taaaaaagct atggatgttt ctactcacta cataaaatat aaaatagatc atcttaaaaa
    31861 agaatttaac attcaaaaag atgaagaacg agtgaagttc gcaaaagaag cttctaaaat
    31921 tataaaacaa ttgacaagtc ctgttgaaat tgatttttat acaaagtatt taagcaatca
    31981 aatagatatt aatgtcgagt ctataaagag agaagtttat ggtaaaaatt acaataagcc
    32041 atataataac aaaaatcaaa agaaaataga agaaaaagta attgaaaaag tcgaagtcag
    32101 acaagacgga aaacaattag ttgaagaaac tctaataaaa ataatgttag aagacaaaaa
    32161 aattagagaa atagcgttac ttaaagtaga agaaagtgat tttttattga aagaaagtaa
    32221 agaaatttta aattacatga ttaaaaatca agaattggac aaaataacta ttgacaaatt
    32281 gaaaagttta aacatatccg aagaatactt aaaagaatta aattcaattt ctttgaatag
    32341 tataaactta gagaacacaa aagaaataga aggaataata acaaatatca gaaaaaactc
    32401 attagaagag caaattaata gcttgttgag agaacaacaa gaattagaaa ataataatga
    32461 tatgaaagag gtagatggta gagttatgga aattgcttta aaaatagttg agataaataa
    32521 aattttaaag agcttgtaga gaaaggaggt ttgagtgtgg aaaataaatc gaataaaaaa
    32581 gagttaaaaa aggttactgc taagacatta atagaaaaag gaaaaaagca aggttctctg
    32641 acacttgcag agataatgga agctttttcg gagactgaac ttgataagga tcaagtagaa
    32701 aatctttatg agactttagg aaatttggga atagaaataa cagaaacaaa aaactataaa
    32761 gctgatatag atttttcggt tgctgatgac gatttaagta taggccactt agatgaagat
    32821 gcagaggcaa tttcacatga tgactcttct gcaatagaaa tagaaactgt ggatttatct
    32881 ttaccaaaag ggataagtat agatgaccct gtaagaatgt acttaaaaga aataggaaaa
    32941 attcctctac ttaaaccaca tgaagaagta gaatttgcta gaaggatgca cgaaggtgat
    33001 gagatagcaa aacaaaggtt agttgaagca aacttaagac tagttgtaag tatagcaaaa
    33061 agatatgtag gaagaggtat gcttttcttg gatttaatac aagagggaaa tttaggtctt
    33121 ataaaagcag tcgaaaaatt tgactataca aaaggatata agtttagtac ttatgcaaca
    33181 tggtggataa gacaagctat aacacgtgct attgcagacc aagctagaac tataagaata
    33241 ccagttcata tggtagagac tataaataag ctaataagag tatcaagaca actacttcaa
    33301 gaacttggaa gagatccaaa accagaagaa attgcaaaag aaatggaaat gacagaagat
    33361 aaagtaagag aaataatgaa aatagctcaa gaccctgtgt ctttagaaac accaatagga
    33421 gaagaagaag atagccattt aggtgacttt attccagatg atgatgctcc agccccagca
    33481 gaggcagcag catattcttt attaaaagaa caaatagaag atgtacttgg ttcattaaat
    33541 gatagagaac aaaaagtatt aaagcttaga tttggtcttg aggatggtag agccagaact
    33601 cttgaggaag ttggaaaaga gtttgatgta actagagaga gaataagaca gatagaagca
    33661 aaagcactaa gaaaactaag acatccaagt agatcaaaaa aacttagaga ttatttagac
    33721 taaaaagata catcattgat gtttttgtga taaatgccat gagataagtg atatctcatg
    33781 gctattttgt agtaaagatt taattttatt tttgtagtta tttcagtcta atatctataa
    33841 cttggattaa atcctataaa taaagggaat tgaaaggaga attatatttg aaattaacag
    33901 atagattatt gaaaatagca tcattagtgt cagatggtaa aaaaatagct gatataggga
    33961 cggaccacgg atacatacca gtatatttgt taaaagaggg aaaagtacca tttgcagtgc
    34021 tagcggatgt taataaagga cctcttgaca atgcacataa agaagtcata cagaacaatc
    34081 ttttagataa agttgattta cgattgggtt ctggtataga gatacttgaa ataggggaag
    34141 tagaagaagt gataattgct ggtatgggtg gaatcttgat aagtgagctt ctcgaggcta
    34201 aaaaagaagt agcacataat gtagaaaaat taatattaca gccaatgcaa gcacaagaag
    34261 aacttagata ttacctttta aacaacggat atgaaatttt agaagaagtt ttagtgagag
    34321 aagattttag aatatatgag ataattgttg ccaaatatac aggtaaaaat actataatag
    34381 aagatgagat atattatgag gttggaataa agcttttaga aaacaaagat tctcttttta
    34441 atgattttat agaaaaaaag ataaagacat atagttctat agttaataaa ttagaaggga
    34501 aaaatgggga agctatagat aagaagagaa aggaaagcga agttgcaata aaaaaacttg
    34561 aaaatttaat aaaataaata ggaggtatat atgctattaa agagtctaac tagaaaaatt
    34621 gaaaaaaagt atcctctaaa cttagcagag gattgggata atgtaggtct tatagttgga
    34681 gattttgata tggatgtaaa aaaggtattg gtatctttag aagctaatga agatgttatt
    34741 gatgaagcta tatccaaaaa tatagattta attgtgacac atcatccttt tatatttggg
    34801 aaaattaata aaattaatag tggagattta aaaggcagac ttatccaaaa acttattaaa
    34861 aatgatatat cactttattc tatgcatacg aattttgata tagcatttga tggtctaaat
    34921 gactatttta tggaaattat ggaatttggt aattcaaagg ttttagatat aactaaaagc
    34981 gaaaatttgt ataaattagc agtatacgtt ccacataact attcagatga acttagaaag
    35041 gttttaagta attcaggagc aggtcatata gggaattata gtgattgtac gttttcaata
    35101 gaaggtgaag gacaatttaa gccattagaa ggctctaatc cgtttcttgg aagtgtaaat
    35161 gatatagaaa ctgtaaatga agttaaaatt gaaactgtag taccacagaa gcttttaggt
    35221 ggggtaataa gttctatgtt agatgcacat ccttatgaag aagttgcata tgatttgtac
    35281 aagttagaaa ataaaggtga gatatttgga ctaggtagaa taagtaaact tgataaaagt
    35341 atgacattag aaagtctttc aaggaaaata aaagagaaat taaatatgaa acacataaga
    35401 gtagttggaa accttagtac agacataacg aaagtagccg ttgtaacagg agctggttca
    35461 gaatttgtaa aaaaagctaa gagacaaggt gcagaagtac taattacggg tgatgtaaag
    35521 tatcatgagg cacaagatgc acttgatatt ggaatgtgta tagtggattg tggtcatttt
    35581 gatacagaag atatattcaa aaatgttatg aagagatttt tagatgaatt tagtgaaatt
    35641 gaagtaataa aaagtaatgt atatttgaat ccatttagta ctatttagca gtgtttgata
    35701 ataaaaatac tatataaaaa ataaatttga gtttaattag ggggataatg atgaaagtag
    35761 cagtaatttt tcatagtgtt tgtgggagta catatttatt agctagagaa tataaagaag
    35821 cgcttgaaga aatgaatatc gaggtagata tatttagagt aagtgacgaa gttgcaaaaa
    35881 cacttcctca atattatttg ataaattcca aagaatacaa agacgaattt gaaagtataa
    35941 atgtaattaa atctggaaaa gaaatattag attatgatgc tatatttatg ggttcaccaa
    36001 catattatgg aaatgtatct ggaccaatga agatgtttat ggatagtttt tctgatattt
    36061 gggtaggtac tcctttgagt ggtaaaatat ttggttgttt tgctacagca ggttcacaac
    36121 atggaggagg agaattagct ttgcaagcta tgaatatatt tgcccaacat atgggaatga
    36181 ctcttttgtc agttccatgt tcagtaagag gaggttatcc agcgtatgga atacttcata
    36241 tagcaggaga taattctgat ataagaccaa atgatgatgc caagataggt ataagagatt
    36301 acctaaaaag acttaacata taagaatatc atataaaaat acctaaaagc gactgtatat
    36361 acttttaggt atttttactt aattataaac gtgaagcttt accagaaata tggtcaattt
    36421 ctattttaac aacagttgtt aagttcatat ccttctctat gtacttcatg ccttcttcaa
    36481 agaatccttt agaatatttt tttattattt ctacaagagc atttttcttt tcttcatttt
    36541 ctacagtgca agcttttcca aaaactatag cactagaata agtagtacta aatttatctg
    36601 gaataacact ttcattagca actactaaaa aagaaacttt gttgtttttc gatatattat
    36661 ctaacttatg cccatttctt gcacaatgaa agtaaataga atcattaaag tatacataat
    36721 ttacagcaac tccataagga taaccatttt cagatatagt ggaaaaagta ccaaattcac
    36781 cattttttaa tacttcaaca gtatcttctt ttgtcatttc tctttttttc aatctcattt
    36841 ctttaaacat aatatcatcc tttctaattt tgatgcttaa attaaatttt agaacattta
    36901 taaagattat gctatgtttt ttacaaattt taagaaaaaa atatagttta agataaaagg
    36961 tagtgaaaga gaagctattt ggaggtttat ttatgatatt attgttagag tagtataaac
    37021 aatactaata aatttaatag ctggtttctt gttatttaag atagtaagca tgtctataag
    37081 aaggggaagt tatcgagggt agaagttgtt atttataact atattataaa gaaaatatta
    37141 ttatataaaa taataaatag taaagaggta tcaagttgaa tataatactt aatacctctt
    37201 caaaattaag ttataaaagt gttgtgggaa ttgtcctata catggtaagg agataataaa
    37261 gaatgtatct ttaaagtctc aactaaacag caaaatttat ccaacaaaca tacaataaat
    37321 gcttctttgt atctaggtgg cttaacagta agtggccaca tgtgttttaa aattatgtcc
    37381 gtttcttttt catttatttg aaaaaataaa gttgcatttt ttaatgcttc tctagggtga
    37441 gtaaatccat gtaatccttt tctatcacct ttgtaatgcc aatcataaag aaaaaagtcg
    37501 tgtagtaaag ctcctctaat aacactttga atatcaacac ttaagtgcaa ttttttacat
    37561 aatagataac tataatatgc tactgacaaa ctatgttcaa gacatgaaac attgccatga
    37621 tgaggaaatg ttctcataag ttgaactttt tcagaagaaa gtatctcaga agcacattca
    37681 ttaaaatatt tgatttcagc ttttgacaac attatgaaaa tctccattct tagtaatata
    37741 taaatactat acttcaacga taatatgaaa tatttcttat cctacaaaat ataataccca
    37801 ataatacaag taagatacct tttatggtat tttaaagtaa gagagatttt attaggctaa
    37861 acaatgaaaa aattaaaagt aaagctatag caatatatag tatttttcca agaggattgc
    37921 caaaatttat agtccatcca ataccaaacc ttttattaac cattaatgat gggtcatttg
    37981 gattattata catgatgcct gctatccaat acttctcatc ttcttctgga gaataagatg
    38041 agttgaattt taaatttggg gaattaatat atgttgctat taaataaatt ataactaaaa
    38101 gcgttgtaat attcatagca gttgatagtt ttgtttttat tgataaaaga gtagtaaaaa
    38161 actgtgttgt cattataatc atcaatatta aaaacaaata accaatttta tttaaatatt
    38221 taatgttttc aactctactt tcttcaattc tatttgtatc tatcttaatc cttgacttta
    38281 tggatccaat ggaagttata tataataaaa ccattaaaca aaagtcagta ccgattaatt
    38341 taaatacatt aagaaatgat ttatctataa aaacatcagg agacccattt atgttccaat
    38401 gagtggcaat tgagtctggc aattgattat aattaaatgc tataaaaata ctcattccca
    38461 aagtaaatag tatcggtatt aaatagatta ttttaaattt ttttattatt ttatttttct
    38521 tatttataaa atccatatct actattaatt ttgaatctat atctatatga cctaatttag
    38581 aataaatttc tgatttaagc ttcttagctt ttttatgtgt atgaatatat agtatacttt
    38641 catataataa aaaacctata ataccaataa aataggaaag ttccaattta ttaaatgtaa
    38701 agaccagaat aaaaactaat ataaagtcta taataaaacc taaagataat agttttttaa
    38761 attctttatc taactttata aagtaattat aatttttatg ctcttggtct atagaaactc
    38821 catagatgtg ttttttacct acaaaatagg gcataaaata aaatgtagca tacatcaaaa
    38881 ttagaactgg aaatattgta aatagacatt caatcttact cataatattt cacctcatac
    38941 ttgtcaaata aacttgaact tagtgacaaa aattcttctt tactaatacc tgataaataa
    39001 gattttgcca ccaataattc caagtcaagt ttattttttt gacgagttgt attatctatt
    39061 ttcattggta gttgagctac aatagcacct tttcttctat caatatttaa gaatccctct
    39121 tcttttaatt cattgtagga tttgttcact gtatgtaaat ttacaccaat attttcagcc
    39181 atacttctta cagatggaag tgattcattt attttcaatt ctcctgaagc tatagcctta
    39241 gttatttctt ctttaatttg tacataaata gatttatcac tattaaaatc taaatttaaa
    39301 accataaaaa acctccatta tgctttataa atgaaaacga ttgttatata catagtataa
    39361 cataaaaaat aataacaaac acaaaatata agtttatatt ttgtaaaaat tgtaatttaa
    39421 aaaataacat aatttttatt aaaataatac agatactatt tatagataga aataaaaatt
    39481 agaaaggaga aaaaatatgt taacatattt aatgaaaaga caaatgcata aaaaagatga
    39541 tggaatgagt aaaccaatga tgattacagg agctgtaatt acaggtgtag gagcatatgc
    39601 tgtatataaa atggtaaaaa gccataaatc taattctcaa atggtcaata caaattatta
    39661 tggaaatagc gattatgatg attataaata tgacgaattt gattatgact gtgattgtag
    39721 agacacaaaa caagattatg aatatgaaga attaaaaaaa aaagtaaaag aatttaattc
    39781 aagaagaata aattgtgaaa attgtccaca tgtttctcaa gaagaaatga tgcaatatgt
    39841 aaatagtgtt aaagatgtaa aggaagagat tgatttacat gaagatgcta aaaaagataa
    39901 tatttacaaa gaagatgaat acaaaaaata tgaagaagaa taagtaatat tttgtataac
    39961 tgaacaaata gtaatgtcaa attatttaat atctagtttt aatatgtaag ttgtaactta
    40021 acttgaaaga atcagtatca ttctttagat agttaagtaa caacttacat tttattttta
    40081 taaattttaa tagttctttg tgactatttt gtaattttag aaatatttta gtatccaggg
    40141 taattgtgta taataaaact tgtatagata gatagtatga aattaagcag atagtttaaa
    40201 attaaaatga aaagaaagga aatagttatg ttaaagttgt tgatagttga agatgatagt
    40261 actatatctt ttggaataaa gtatgcttta gaacaagaag gatttagtat agatatatca
    40321 aaggatttat ctagtggaaa agaaatgatt tcttctaatg aatatagcat gatactgttg
    40381 gatgttacat tgccagatgg aactggttat gagctttgtc aatatataag aggattctct
    40441 caggttccta taatattctt gactgcttgt gatgaagaag taaatattgt aatgggtctc
    40501 gatataggtg gagatgatta tataacaaaa ccttttagaa ttagagaatt aatatccaga
    40561 ataaaagctg ttttaagaag aaaaggtaat acttcagaag aaaataagaa aatacttaaa
    40621 tttggagatt taagtatata tacattagaa gctagagtat ataaaaatga taaagaaata
    40681 tttctgacat cagttgaata taaacttctt ttaattctta tacagaataa aaatactgtt
    40741 ttaagtagaa ctcaaatatt ggaaaaactt tgggatgtaa cttatgattt tgtaaacgac
    40801 aatactctaa ctgtttatat aaaaaggctt agagaaaaaa taggtgatga tttatgtgaa
    40861 ccaatctaca tagaaactgt aagggggatt ggttataaat ggataggaag tgaaaatagt
    40921 gtttctatat aatccagaag tgaagaagtt tttaagtaag tatgttactt tgatgtttat
    40981 tgttatcata atatctattg gatttagcat aatcaacgtg tccttaacta aagagatggt
    41041 tgtaagaaat aatcaggcta taataggaac tttatcaagc aagtatccaa acttggaaag
    41101 tcagatagta gacataataa ctcaagggaa gtctatggag aacatagatt atggtcagaa
    41161 aatattaatt aagtataatt atgataaatc tattaggata aactctgagc ctattatttc
    41221 taaattagtt ttagagacta tcaaaataaa tataatatta gtatgtataa tatttatttt
    41281 gatatttgtg ttggttgtta gatattttaa gtcaatttac aatgatttat cagatatgac
    41341 taagtatgtt tactatagtt cagagggtaa gccatttgat atgaaaaaca agaatcaaga
    41401 agggcagata ggtcttctaa aaactgaact cttaaagatg acaacaattc ttaatgaaaa
    41461 ggttgaactt ttaaaaagag aaaagatatt tttaaacaat acaatttccg atatatcaca
    41521 tcaattaaaa actcctatga cttctttaat aatgttgaat gatttattat ataatgatgt
    41581 accatacgaa gtaaaaatcg attttttaaa taagataaaa aatcaattaa atagaatgga
    41641 ttggttgatt aaaagcatgc ttaaattatc aaaggtagaa gcaaaagtga taaattttaa
    41701 aaaagataag gtaaaattta gtgaactcat acatagagca atgctatcta tgaaaatacc
    41761 tatggagatt aaaaatcaaa aactgactat agagggaaat gataatgtat cttatattgg
    41821 tgatattgat tggtccgtgg aagcattagt gaatataatc aaaaattgtg tagaacatac
    41881 tcctgaattt ggaaatataa ttataaccta taaagaaaat ccattatttt ctgaactaat
    41941 aataaaagat gagggtgaag gtatagataa aaaagatata ccacatgtat ttaaacgatt
    42001 ctatagagga agaagtagtt caaaagaaga tagtgtagga ataggtcttg caatgtcaaa
    42061 atctataata gaaagccaaa atggcgatat atatgtaaac agcgaaaaag gtcagggtac
    42121 agaatttcat ataatatttc ataaaatgta tgatagtgac taatctgtaa tatttaattc
    42181 actttatagt aatagccaaa acctaatata tgaagtatag aatataaata ttggaggtag
    42241 ttatggaaat tttaagagta gaaaatctaa ctaaaagtta tggtaaaaat gaaacaaaag
    42301 ttgatgctat aaaaaatgta agtttatcag ttgaaaaagg aacgtttata gcaataacag
    42361 gtccaagtgg aagtggtaaa agtaccttat tacatttatt aggaggagtt gacagaccaa
    42421 ctagtggtaa ggtttatata aatgatgttg atatatataa tttaaaaact aaggatttgg
    42481 cgatatttag aagaagaaat ataggtctta tatatcaatt ttacaactta attccagtct
    42541 taaatgtgaa ggaaaacata ttgcttccag cagagcttga caatagagat attgatagag
    42601 aatattttga tgatttaatg aaaacattag gtctaattga tagagaaaag caccttccaa
    42661 accaattatc aggaggtcaa caacagagaa cctctatagg acgtgcatta attaatagac
    42721 cttctataat attagcagat gagccaacag gtaatttaga tagtaaaaat tcaaaggaaa
    42781 tattagaatt gttaaaattg tcagtaaaaa aatacaacca gacactaata atgataactc
    42841 atgacaaaaa tatggcactt caagctgata gaattataag catagaagat ggaatcataa
    42901 aaagtgatga ggtgatgtag atgaatttat atacattact aaccattaga tacttaaaac
    42961 aaaataaaag aagaaccatt gtaaccataa tcggtataat actttcaaca gctttaattt
    43021 gtggtatagg aaatatattt gaaagtttta tggattatca aatgagagaa actataaaga
    43081 atgatggaag tttccatgtt acattttatg atgtaaatag aaaaaatgtg gaatatgtaa
    43141 cgaaatcagc tgaaatagaa aaacatgcat ttaccaaaca acttggatat tctaaattag
    43201 aaaatagtga aaatgctata ttaagtataa agcaatatga taaaaatgca ataaatggat
    43261 ataaagtttc tataaaagaa ggtagatttc cagctaagga aggagaaatt gttctaagtg
    43321 aaagtattat taatcttata gatgataagt taaaaatagg agataagata actttaaaag
    43381 taggggatat gtttgactca agcaagaaaa agattgatag tatgacattt catgaaggtg
    43441 attatatagc aaatgaaaaa gatagaactt ttaaagttgt tggtataatt gaaaaacctg
    43501 ggtttgaacg ttataatgga attgcaacag ctatagccta ctttaatcct atgaataatt
    43561 acaatgatac aattaatgta tcagtagcag taagaaatcc taaagatgtg tataagataa
    43621 gtaataaaat aagtaaaaat atatattcaa ataaagataa agaagcagtt tctgatatga
    43681 taaaatacaa tgaacattta ttgaggttac aaggtgctag taagtattca aatataaata
    43741 actctataaa gtctataatt atagcagtaa ctattttagt aatcatatgt acaattgcta
    43801 cagtgtacaa ttcctttagt atttctataa atgaacgaaa aaaacagttt gggatattaa
    43861 attctatagg tgctacatct agtcaaatta aaagattagt ttttatagaa ggtattataa
    43921 ttagtttaat tggtatacca ataggactaa tatctggaac agtagctata gacctattgt
    43981 ttaaaattat aaataagtat tttactgaat cggttgttac taagatgagt ttgcagatag
    44041 tgtataatcc aataataact attgttagta taataatagt tttgcttaca atctttatat
    44101 caattctact acctgcaata tcggcttcta atatttctca ccttaatgtg attaaaaata
    44161 ctggagagta taaagtgggc aaaatcaaaa gctcaagact tattaaaatg atttttaaaa
    44221 ctgaaggagt acttgcatat aaaaatatta ggagaaatag aaaaaaattt ataataactc
    44281 ttttttcttt aatggtaagt ataataatat ttatatcttt tagtggcttt acattacttt
    44341 tactaagagg tgaagaaatt agaaattctc aaagaaaata tgatttgtat ttaactgcaa
    44401 agaacacagc taggtctgta gaggatacta taagtgaact agaagatatt gatgggataa
    44461 aaaactttga attggcaaca ggacaatatg tgtctataag agtaagtgaa aataaaataa
    44521 ataaatccaa ggaagattta atcaggaaat attatcaaaa atataagatt ggagatagtt
    44581 atgaatatga tttcagtaat aatgaattaa tttttccagg agattttgca gtaaaaaaca
    44641 aaataaataa cttagtacag ggctctttta ataaagagag agcaattgaa gaaaatggtg
    44701 ttatattagt tagaaaaagt gcatttgaag aaaatgggaa gaaaggtgta gtagaactta
    44761 ccaactataa agtaggagat acagtaaatt gtgaatatct tgatgaaaat gccttaagta
    44821 aaaaagtaaa ggtcaagatt cttgctataa ctgacgaaga acgtctaggt cttggttatc
    44881 aaaatatggg gttacaattt ataacttatg atgaagttgc taaaaattta aatttaaaat
    44941 taagtaaaag ccttatattt attgattcag gaggaagcat taagactaag aaggaaatag
    45001 aagctttggc aaataaaaat aattttaact tttatgatga aagtgcaagc aatgaaagtg
    45061 aaaaacaaga tttaaaggtt attaaaatat ttgtatatgg ttttatagta gtgatatctt
    45121 tagtgagtgt tacaaatatt ctaaatacag taagtacaag tataaatcta agaaaaagag
    45181 aacttgcagt aatacaatct ataggggtta ctcctaaagg atttaggaaa atgatatatt
    45241 tagaaagttt tatatatggt atactgtcat tgttgtttgg aataccaatt agtattggta
    45301 taattctaat tatgaataaa cttatttcag gtgtaattga attttctccg attataccgt
    45361 ggacagccat agttatctgt atagtaagtg tttttataat aacttttata gcagggtata
    45421 taccaatgag taaattaaat aaagaaaata taatagataa tataagaaga gaaagtatat
    45481 agatataaaa ataattctta actatataaa atattattaa aatcatttaa gtggtataaa
    45541 atattattat atcacttaaa tgatttttta tttatataag aaatctaata ataaattact
    45601 agaggtattt aactatttag aaagaattgg tattatataa agtaagagaa tatataataa
    45661 gaaagaatat ttgattttat tggaaaacaa aagaaaaaga aggtgattac tatttctcat
    45721 ataactgcga agaatatgta taaaagttta gaggaaagaa taaacaagtt tccacaaggt
    45781 gctccaccat ctgatacatt atacaaaata ctaaatgtac tatatacaga acaagaagcg
    45841 aagttagttg ctcagcttcc aataaagcct ttcagagtaa aaacagcagc taaaatttgg
    45901 agtgtaagtg aaagtgaagc atatagagta ttggataaac ttgctagtaa ggctctgata
    45961 ctagatatag aagataataa agggaagaaa tatataatgc ctcctccaat ggctggtttt
    46021 tttgagtttg ccatgatgag aacgagacac gatatagacc aaaaactttt ggcagagctt
    46081 tactatcaat atatgaatgt agaagaagat tttatcaaag atttatttta ttcaacagaa
    46141 acgaagcttg gaagagtata cgttcaagag gaagttttaa ctaatgataa tgaggttagt
    46201 attttagatt atgaaagggc tactcatata attgatgaat caactcatat aggtataagt
    46261 atgtgttact gtagacatag aatgcaacat gttggaaaag cttgtgatgc tcctatggat
    46321 atatgtatga cgtttgacaa tgtagcaaat tctttaataa ataataaatt tgcaagacgt
    46381 gtggataaaa tagaatgtaa agaactactt catcaagcat atgaacataa cttagttcaa
    46441 tgtggagaaa atgttaggaa aggggttacg tttatatgta attgctgtgg atgttgctgt
    46501 gaagcaatgg tagcagctaa aagatttgga aatcttcatc cagtacagac aacaagtttt
    46561 ataccaaata taaaccatga aaattgtgta aagtgtggta aatgtataac tgcatgtcca
    46621 atagatgcga taagcaaggt taaggaagat ggtaaagaat atataaaaat tgatgaggat
    46681 agatgtctag gttgtggagt ttgtgttaga aattgccata aaaatagcat tatgttatta
    46741 aagagagatg aaaaaataat aactccagca aattcagtac atagagcagt tcttatggca
    46801 attgaaaaag ggcagttaca aaatttaatt tttgataata atgctctggc tagtcataga
    46861 gctatgggag ctatactgtc agctatatta aagcttgaac cagctaaaaa gattttagcc
    46921 agtaaacaat taaagtcagt atatttagat aaattgttaa gtatgaatga taaatagaaa
    46981 ctaacttaaa aaaggcaaag ttttagactt atctatatct taataaactt atcatttaat
    47041 atttaaattt aatttttaca catataaaac ttataactta aataaaaaaa ggctatattc
    47101 aattaatatt acttgaatac agcctttttt agttatgtta agctatttct tttatttgct
    47161 taaatgagtt ttcatttcca tttccaccaa tttgtgttac agatttaaat ctcatagttt
    47221 taaatagttc tttttgatta taatctagtg agtttcctac tagcattaca ggagatttag
    47281 tttttccagc caaaactcca acagataatc cgtctattaa atcatcttgt ttattcattc
    47341 catttttagc aacatataaa ttgtctatct tagaatcctt gtaaaattct tttattactt
    47401 ttgcattagt ttcatttcta gtgctacctg atattctctg tggattttga agtttacttt
    47461 ctatattttt agaaactgta tattctccac cgattacata agatttatca atctttttgt
    47521 ttttaaacaa gttatttatt tcagttatat tactattttc attagtaagt attataggca
    47581 tatcattttg agctgataca gctccaatgc ttactgcatc ggctaatcct ttttcgccat
    47641 taactactga aatctttgat atattgctaa tattgttcaa ttctttagca atgttcatag
    47701 aagtttgata tctatcactt cctgaaattc tagatacaga gatattgttt gattttatag
    47761 tatctaaaga tttttcattt atagaagctt ctccaccaac tacatataca tttttaactt
    47821 ttaatctctt taattcagcc aaagtttttt catttatttg agagcttcca gtcaataata
    47881 taggagcgtt tttcttgtat gcaaatggtg ttgcagccaa agcatctgca attgctgaat
    47941 cgtttattaa aactgcattt tcagcatttt cccaacctgc ttgacttatc ttaatagaag
    48001 tttcatatct atcagttcct gtaagtgcac ttgcatttac ttcatcttga tatgtaacct
    48061 caaatctagc atttggactt cctaagaaaa caattacgcc tccttttggc aatctaactc
    48121 tattgttctt tgtaactaaa gaatagttta caggaacacc tttatcatca taaacagcaa
    48181 aagctgaatt ttgaggaaga ttaacttcta tttttttatt tgcgatatcg tcaccaatct
    48241 tataccattt tgcataacca ttggattcta gttcacaagt aaaagatttt tctgctggca
    48301 aattagttat agaatcttca ctaacatacg ttagtgttcc aaaagataaa tactctgtac
    48361 cattttcttt atgaagttta atgtcggata aatcacgacc tattacacca ggtatttcta
    48421 taaaagcatt tgagttattc tcatccatta ttttagtgtt aactatataa cctggagttt
    48481 catcagaaag actaaaactt gcttttacag agccaaacat atagctttga gaagtatatt
    48541 tttcatccac tagatagtaa cctttgccac ttcttttttt ccacacttct ttgacactat
    48601 ttgatatatt gttgtcatct actttttgag ctacatagta taaacttgct gtctgaccta
    48661 atccagggac atcatcataa gagctcatat ttaaatatgt gatattattt ttttctttta
    48721 caaattttaa acaactatta cctttttcag atacaaatct atcttgacct atgtatacgt
    48781 atttatcttc aggtccattt tctatataag gagaagatac tgttaaagta ccattatcat
    48841 taacatctac ttttatcata tttgaagaag cataaagacc actattttct tttaaagagc
    48901 taggcatttt aacttgttgt ggcttactaa atgttttatc aggttttatt tctttaattt
    48961 tacctttttc ttttaatgca gatagtaaga tttcttgtcc aattatttca ttaagttgac
    49021 tacttccacc agaagaaaga actgcaactg ccatattttc atctggaaga actataagat
    49081 tactgtggaa taataaagaa tctccacctt ttgtaagagc ttttaaatta tactgattaa
    49141 atggataagt gttgacacaa tcccacccta aaccatatcc aagtatagag tcttctcctt
    49201 caggccatag accatttaaa tattctttgt tttccatagc ttttactgaa gcaggagaaa
    49261 gaataccatt tgaatttttc ataaatgttt gtgcaaatgt acatagattt tctgcacttg
    49321 aatataaacc acctgcacct atagcattaa ggttatcttg tggaactgca tcttcccaat
    49381 aaggtacata tgcttttgct agttttgaac tatcaaaact attttctgga gtttttgtat
    49441 tttgtaagtt aagtggatta tttatgtatt tatcaagaaa attagtaaat gacataccag
    49501 atactctttc tactaaaatt tcagctaaag taaatccatc attacaataa acagaaaaag
    49561 ctcctggttt agcttttaat ctttgttttt gtaattcttt taagaaatta tcatgaccat
    49621 aagaatcatt gtctgctaat aaaatagtgt ttttaaaact acttcccatc aaccctgatg
    49681 agtgatttaa taacattctt ggagtgattt ccttgtatct atcatcagcc attttaaatt
    49741 caggtatata ctttacaaca ggagtatcta aatttaattt tccatcatca accaacttca
    49801 taacagctgt tgtagtaaac attttactga tagatgcgat actatacata ttatccttat
    49861 ttaagttttt gttatcctgt ttgctgtaaa ctccaccatt accagaaatc tcaattttgc
    49921 cattatcaat caaagcatat tgagcactta cagcaccata ctttgatagc aaagtttgga
    49981 ttttctcttt agcaagagct ttagtttttg tgtaagattc cgcgttttta ctataatcat
    50041 gtaattctat atcagaactt tgatattttt cggaattatc ttttgtatta tcagcaaaag
    50101 taatatttgg agcagcagta aataacatac tcaatactaa tgtactagat aataatttag
    50161 agagcctttg cttaaacata ttacattacc tctttccctt tttaataatt tataattagc
    50221 ataacattct taattatttt aatcaacaga ttattatcaa taaaaaaagc ttttataagt
    50281 atagcttttt atataacaga gaaaaatata aaaataatat ttgttgttgt ataaagaata
    50341 aagggtatag gtaatatata aataaaatgg aggaatttta gacatatgta taagaatata
    50401 aataagaagc aacttaaaga aatgatgaaa aatgaaaagg atgttttatt attagatgta
    50461 agaactaaag atgaatttaa ggaatataaa atagaaaatg ctataaatat atctttacaa
    50521 gagttaatca ataatataga tgaaatttac gattataaag ataagaaagt agtagtttat
    50581 tgcagaagtg gtcatagaag tgttactgca tgtaatttgt tagctgaaga aggttttgag
    50641 catttatata atttaaattc aggaataatt gattatatga gttaaataat gtaagttata
    50701 cattaaatac tatgtatagc tctgcttatt aagtaggaat atatctaaat atttataaga
    50761 taaatttagg aaagaacgaa ttttttcgtt tttttctatg acagtttttg tagtaaatat
    50821 tagtttttaa gttggaaaaa ggtagtagct ttttagctac taccttttaa tatgaactta
    50881 gaatattatt aaaaaaatat atttaaagac agaattaatt ttttaatgac cagttaaatt
    50941 actatcgccg aatttatcat taaatggttt tataactcta tttataggtc cttttagaag
    51001 tacatttaaa caaatagaca gaatcataaa tagtaataga actagtatat ctttagttag
    51061 atttggagca tatataccac caactgcttc ccttgtagca cctattgcat aggtaaatgg
    51121 cattataggg tttattcttt ggaagaatgt aggtgtaact tgtataggga atgtaccacc
    51181 agaagaacca atctgaacga ctaatagtac tattgcaatt gcttttccta catttccaaa
    51241 tacagaaact aaagaataca ctataaagtt aaataccata ctagtaaaca taagcagaac
    51301 tatcaataaa attggatgtt ttgctgttac tcctaacaat aatatatctc cagttcctat
    51361 tataaaacct tggacaagag ctatacttaa aaaagtaagg cctcttccaa agtaaatttc
    51421 ataaggttta tagtttccat gtacagatgt agacaatagt gctgaaataa gtaatatacc
    51481 aacccatgta gaaagaacac tataaaatgg tgtcattgca gaaccataat tttcaattgg
    51541 atataatttc tctgttttca cctcaacagg ttcttttaag aaattagact ttgtcaatat
    51601 atcactttca agcaagctga ctaatttttt catatcttca ccattactaa ttttgcttaa
    51661 agtatcaatc aaatcatcca acattccttt tgctaaagga agtctttctc ttatcaatga
    51721 tatactttcc tgtgcatttc ctgaaagttt tagagaagta gtaagtattt cttcaacctt
    51781 tggaagtttt gcttctgcct tatctaaaac agtaattata tcatttgcta cttttatact
    51841 gtttgcaaat atactattta taggttttga tatttttgag tcaaaattat ttaaaatatt
    51901 taaattaatt cttccaatac catttgatag acttaaaaca ttattcaaag cacttataga
    51961 tggttgttgt ccagatataa ctttgttttt aatatcattt aatgttgata tagatgaatc
    52021 tattttattg ctagagtctt caagattatc tatgacatca tccaatctat tatttgatgt
    52081 aagttggttt aatttagtta aaaaatccac caaagtatca tttaaagatt gcaaattaga
    52141 aagtttttct gataaattat ctatcaactt aggtacatct tcactgccac tatttactgc
    52201 atcaattaaa tttaatgtaa gagaagaagc agaagatgaa aggtcaacca ttaaatttaa
    52261 gtcagactta ataagaggag aaagttcgtc taaattatca tttgtatcct ctaagaattt
    52321 ttttaagtca cttgataata gttttgtatc attcagagtc tttttaattg taggtaaatc
    52381 agatttaata tctttaactg tatcagataa ttttgaagta gcatcagaag ctaaatcaac
    52441 agtcttattt atatggtcaa atctcttttg aaggtcaata agggaatttt caacttttgt
    52501 caattttgga agactgtgtt caattcctgc accaactccg tttgatatac caagaacgat
    52561 ttcactaact gtttttacta tagtctgatt aacctgtagt tgtattgtag aagcaccttt
    52621 atctgtaatc tttggtgcaa ctgcatttat tttttcatta acagtataga taatttcgcc
    52681 ttttttaaca tcatctgtaa ttaaagatgt caaatctttt gtaaaatttt ttggaatctt
    52741 aagtgatgca taataagtac cttttttaac accttcaaga gcagtttttt catctacaaa
    52801 ctgccaacct aaactcttgt tatctttgag ttgttcaatt aattggtctc ctacattaat
    52861 cttttgtcct gctatttcat ttcctttatc ttcatttgtt actgctacaa gcatattagc
    52921 agtgcttcca taagggtccc aagatgcctt tatattgaac caagcataca gagatggtaa
    52981 aaaagcaaga cctaaaacta ttacaagtac ggcataattg ccacgtatat cttttaggtc
    53041 acttttaaaa atttctaata tattttttaa accctttgaa ggtcttaaat tcttgatatt
    53101 attccagttt aaaatttctt ttaaactatt agtttttttc aaatgtctca cctcctaaaa
    53161 tattttgata aagaaaaaag ttagatttat aatcaatttt aaattatatt actttttaca
    53221 caaatagtaa agttaaaatt ttgtc
//
LOCUS       DAMJWU010000014        53055 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMJWU010000014 DAMJWU010000000
VERSION     DAMJWU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.014"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(<1..884)
                     /locus_tag="PC460_001009"
     CDS             complement(<1..884)
                     /locus_tag="PC460_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2599906.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQTMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPSS
                     LDTESSIKQGCKYFASLLSSCKNQDIDDLNVAIQSYNYGGGYVGYVAGNGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVFGGSSPTTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVT"
     gene            complement(881..3085)
                     /locus_tag="PC460_001010"
     CDS             complement(881..3085)
                     /locus_tag="PC460_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2599907.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            complement(3085..5535)
                     /locus_tag="PC460_001011"
     CDS             complement(3085..5535)
                     /locus_tag="PC460_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2599908.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYEMIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(5513..5911)
                     /locus_tag="PC460_001012"
     CDS             complement(5513..5911)
                     /locus_tag="PC460_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2599909.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            complement(6029..6532)
                     /locus_tag="PC460_001013"
     CDS             complement(6029..6532)
                     /locus_tag="PC460_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2599910.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            complement(6550..7053)
                     /locus_tag="PC460_001014"
     CDS             complement(6550..7053)
                     /locus_tag="PC460_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2599911.1"
                     /translation="MQETRVLVETGRTNGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSADMQEEYVELLTVFENLEALYICR
                     NVITVYHDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENKRYLVTEN
                     SIFELPE"
     gene            complement(6972..7268)
                     /locus_tag="PC460_001015"
     CDS             complement(6972..7268)
                     /locus_tag="PC460_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2599912.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     KDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGYWWKREEQMAKKQHHTGLTYR"
     gene            complement(7367..8161)
                     /locus_tag="PC460_001016"
     CDS             complement(7367..8161)
                     /locus_tag="PC460_001016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599913.1"
                     /translation="MGTINYFLKGIKVLMISDEMRVDILHAVFINKNFIKDEENCNYE
                     KYLLELVNKSIYFREKSNFAEYVPPKSENHGECDCNSPNYQMDFKLLESTTRLHASKE
                     LTGQIQKFCDGVIGKCPPRRPNTQMTVTRLFASLRDYDCESLHSCLTEKYEYGTIEFD
                     IQTYVKLLTFKKNLFFFFPYKFSFNTCYNFKYALDSIRIALEKDFRESNLFREKYYAE
                     YDTFLAYIYEDNLIISKFEQDGKLQMIDCIYLFKSQTYSKLYEYTW"
     gene            complement(8200..8421)
                     /locus_tag="PC460_001017"
     CDS             complement(8200..8421)
                     /locus_tag="PC460_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599914.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(8422..8556)
                     /locus_tag="PC460_001018"
     CDS             complement(8422..8556)
                     /locus_tag="PC460_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2599915.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSISKEADYEMKQLEESEDN
                     "
     gene            complement(8569..9765)
                     /locus_tag="PC460_001019"
     CDS             complement(8569..9765)
                     /locus_tag="PC460_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2599916.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGIMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKYYQSGELIKHREDDR
                     EYMGSTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFIDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAILSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(9950..11344)
                     /locus_tag="PC460_001020"
     CDS             complement(9950..11344)
                     /locus_tag="PC460_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2599917.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSVFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNLRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGFLAQARQ
                     DGTATCEAKGDGSD"
     gene            complement(11438..11875)
                     /locus_tag="PC460_001021"
     CDS             complement(11438..11875)
                     /locus_tag="PC460_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599918.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFNVGEYKEYPHTEVRKLTSDEIKQYMSER"
     gene            complement(11977..12360)
                     /locus_tag="PC460_001022"
     CDS             complement(11977..12360)
                     /locus_tag="PC460_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2599919.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            complement(12376..12702)
                     /locus_tag="PC460_001023"
     CDS             complement(12376..12702)
                     /locus_tag="PC460_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2599920.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVEEK"
     gene            complement(12828..15872)
                     /locus_tag="PC460_001024"
     CDS             complement(12828..15872)
                     /locus_tag="PC460_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2599921.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPSTAVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDKNGNTDVVEVKAGTVYIRELSAPAGYKVDTT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAILPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETSDTKPQGSATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSAEWTSHK
                     HNTNAGKTSEDGVWFGTSEADDSKGALPYDTYIIEEMRCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDSEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFIADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKEIEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKAEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFNEKPEVPEKLETPQTPEKPN
                     RPSDSPKTGDSTNVMAFLVMLLTSAGGLAGTYLYKRRTMKKS"
     gene            complement(16115..17479)
                     /gene="rlmD"
                     /locus_tag="PC460_001025"
     CDS             complement(16115..17479)
                     /gene="rlmD"
                     /locus_tag="PC460_001025"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2599922.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHIETCVLLQ
                     RRTM"
     gene            complement(17654..19414)
                     /gene="pyk"
                     /locus_tag="PC460_001026"
     CDS             complement(17654..19414)
                     /gene="pyk"
                     /locus_tag="PC460_001026"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF2599923.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(19466..20425)
                     /gene="pfkA"
                     /locus_tag="PC460_001027"
     CDS             complement(19466..20425)
                     /gene="pfkA"
                     /locus_tag="PC460_001027"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2599924.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(20634..24203)
                     /locus_tag="PC460_001028"
     CDS             complement(20634..24203)
                     /locus_tag="PC460_001028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF2599925.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(24360..25307)
                     /gene="whiA"
                     /locus_tag="PC460_001029"
     CDS             complement(24360..25307)
                     /gene="whiA"
                     /locus_tag="PC460_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF2599926.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(25324..25749)
                     /locus_tag="PC460_001030"
     CDS             complement(25324..25749)
                     /locus_tag="PC460_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2599927.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(25818..26924)
                     /locus_tag="PC460_001031"
     CDS             complement(25818..26924)
                     /locus_tag="PC460_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF2599928.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(26936..27793)
                     /gene="rapZ"
                     /locus_tag="PC460_001032"
     CDS             complement(26936..27793)
                     /gene="rapZ"
                     /locus_tag="PC460_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF2599929.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(27824..28537)
                     /locus_tag="PC460_001033"
     CDS             complement(27824..28537)
                     /locus_tag="PC460_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2599930.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(28541..29455)
                     /gene="murB"
                     /locus_tag="PC460_001034"
     CDS             complement(28541..29455)
                     /gene="murB"
                     /locus_tag="PC460_001034"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF2599931.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(29728..30498)
                     /locus_tag="PC460_001035"
     CDS             complement(29728..30498)
                     /locus_tag="PC460_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2599932.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            30816..32297
                     /gene="cls"
                     /locus_tag="PC460_001036"
     CDS             30816..32297
                     /gene="cls"
                     /locus_tag="PC460_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2599933.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            32615..33112
                     /locus_tag="PC460_001037"
     CDS             32615..33112
                     /locus_tag="PC460_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF2599934.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            33129..35015
                     /locus_tag="PC460_001038"
     CDS             33129..35015
                     /locus_tag="PC460_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF2599935.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            35038..36819
                     /locus_tag="PC460_001039"
     CDS             35038..36819
                     /locus_tag="PC460_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2599936.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(36959..38755)
                     /locus_tag="PC460_001040"
     CDS             complement(36959..38755)
                     /locus_tag="PC460_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF2599937.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(38969..39898)
                     /locus_tag="PC460_001041"
     CDS             complement(38969..39898)
                     /locus_tag="PC460_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF2599938.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(39898..41715)
                     /gene="uvrC"
                     /locus_tag="PC460_001042"
     CDS             complement(39898..41715)
                     /gene="uvrC"
                     /locus_tag="PC460_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF2599939.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(41974..44799)
                     /gene="uvrA"
                     /locus_tag="PC460_001043"
     CDS             complement(41974..44799)
                     /gene="uvrA"
                     /locus_tag="PC460_001043"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2599940.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(44818..46788)
                     /gene="uvrB"
                     /locus_tag="PC460_001044"
     CDS             complement(44818..46788)
                     /gene="uvrB"
                     /locus_tag="PC460_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF2599941.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(46975..48237)
                     /locus_tag="PC460_001045"
     CDS             complement(46975..48237)
                     /locus_tag="PC460_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF2599942.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            complement(48300..49604)
                     /locus_tag="PC460_001046"
     CDS             complement(48300..49604)
                     /locus_tag="PC460_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2599943.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(49635..50495)
                     /locus_tag="PC460_001047"
     CDS             complement(49635..50495)
                     /locus_tag="PC460_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2599944.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(50539..51408)
                     /locus_tag="PC460_001048"
     CDS             complement(50539..51408)
                     /locus_tag="PC460_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2599945.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(51392..52462)
                     /locus_tag="PC460_001049"
     CDS             complement(51392..52462)
                     /locus_tag="PC460_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599946.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            complement(52917..53033)
                     /gene="rrf"
                     /locus_tag="PC460_001050"
     rRNA            complement(52917..53033)
                     /gene="rrf"
                     /locus_tag="PC460_001050"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        15549 a        10396 c         7558 g        19552 t
ORIGIN      
        1 gttacatacg aaccagcgtt ataggtggaa tggaaaaata ccaaatcgcc agcctttgcc
       61 tgcgagagtg gaagatgttg ggtagcgtca tactgggctt gtgccgttct cggtaaggag
      121 ataccagctt ttccatagca ccattgcgtc agtcccgaac agtcaaagga agtggttgga
      181 gaagaaccac caaacacata tttccaccct tgatatttca acgcttcatt cattaccttt
      241 tgtgccagct ctcccgatac ctgcggaaca gttaaatact gattgactaa ttccacatag
      301 aacatatttc catagccata ccgccagcct ccgttcttcg caacggctat cgggttggta
      361 taggttactt tctttccacc cgatttctca cgggcgaagc tctctgcaag attaaaagtg
      421 tgtttctttc cgtttcctgc cacatagccc acatagccac cgccatagtt ataggactgt
      481 atcgctacat tcaaatcgtc gatatcttga tttttgcagg aagaaagcag ggacgcaaaa
      541 tacttacacc cctgcttgat tgagctttcc gtatctaagg aactaggcgg taaaccaaga
      601 ctttctgaac tctgcataac atcttctgcc gtaccgccac tttctacttg aatgatagcc
      661 agtagtacat tgacgtactc ggagataccg ttttctctgg cgtatttttc taccatagtc
      721 tgatgtttca aaacttctgc ggataagttc atacccgtaa tgccagaaga aaagttgctg
      781 ttttcgtcgt cgctgtccgc actaatcagc accccaaaga aaagcaccag agaaaagaga
      841 ataggaaaca gacttccaat gatagcgata tgtttcagtt tcattttttc ttctgtcctt
      901 tcttcgttac aagattacgg attttctctg tggtctgctg gttcttctgg acagtctggg
      961 tctgctgaac ctttgtccta cggtctttgg tgtaattctg gcgtgtttcc tttgacacgg
     1021 taactttttc tacattctgc ctatatgtcg attgggtcgc tttcccgtca ggagtactag
     1081 aagatagcgg acgttctttg acaccgcttg tcttaactgg ttcgcttgct ttcgtagtcg
     1141 gagttgtggc agggcgtttg acttcctgca ttttttccgc acccgtcttt gaaacggttg
     1201 aagctgtggc tggtcgctca tggggacggg tagctcccgt tgtcgctgat ccgtcagcct
     1261 ttcgctgtgc ctgccttgct tcttgtgcct tttgaagctc catacgcttg acagcgatac
     1321 ttttcttatg ctgttcctgc ttttccaagc gtcccgtctg tctggactgc tgttcctgca
     1381 ccataccacg cttgaaatca gacacgctgg actttgcctt ttcctttgcg gaatacaccg
     1441 cataagcggt ctgtgtgggc atatccttga tattctcttt gacagcgttt gccttgtctt
     1501 tcactttatt tttcgtatct aagacagcac ccacctttga gcctgcacgc tgtcccatac
     1561 tggaagtggt attgccccga ttttctttag aagccgtatt ttttctttcg gctctcttgc
     1621 cagcaacggc acttccagct accgcaccag ccacaccgcc cgaaataccg ccagcactta
     1681 cagcccttgc aatacggtgt tccatacgcc tagcccgatg tcgcatgaac agatacggtc
     1741 tgcggaatat cctgcgtccc atgctttgac tgtcgccagc gttgagagaa aacatactca
     1801 ttaagtcgcc cagcttcata tagataccag cgaaacatac tatctgcaag aacgccacca
     1861 tgaaaaacgg atagtccgtg gatatgttat aaaacatact ggaaatgcta aacgcaaccg
     1921 ttacaatgag cgttattcct gcccgtgtca ttatggtatt aaataccctc acgattgcct
     1981 gctttgccat gctttcatag ctcggtatca tggaaagtag aaagctgata ggtaaaaaca
     2041 tagcgaagat aataaaaagt atctggctga acaacatcat acccgtaagc aagaatacaa
     2101 atatcgtaat accaagatta aagaacaaga ggaagaacac catacctaaa cggtttacca
     2161 cctgcggtat cgtcagattg ttgttgtcgt tgtcctcgat ttctgttttc acgacttctt
     2221 ctctggtctt tccgtcctcg tcctctggac ttgccgatac gagagcttcg acacggtctg
     2281 tcccgatttc ctctgcgttg ctgtttccaa actgtaagag tagccacggc tgttgtactt
     2341 gaatagaaaa taggctgtcc cgtatcaagt ctacgctgtc cttgccctcg ctgtcagagt
     2401 tggggagcat gatttttgtt cccaaatcaa gcgaagcggt actgatgtct gatgaaaatt
     2461 catttatctt cttgatgtag tcgggagcgt aggcaataaa cgaagcggac agcacgaaca
     2521 ccaccacaaa gttgataaca gcgtgaagtg ccttgctggt ttcccgtttt atcagtcccg
     2581 tataggcaac atacattccc accactaaga taataagaag caggaaacca acatagaagc
     2641 ccgtagaaga aaagccgttc tcggtaacgc ctgcaagggt ctgtatgctt tttccgatac
     2701 tgtctgccat atcgttaata aaatcaagtt tataggcttc ctgcaccaca tagccagttg
     2761 cattgcttaa ataaaggctt atcgtccaga caaagttggt aatgcagtaa agcccgtact
     2821 gtaccgattt tccgataccg tccagccagt tccacggaag ccacgaccag ctattatcga
     2881 cgaaaaaatc aagctggtag ttggaaagcg gatattttga atatagattt tctgcgttta
     2941 tggtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa
     3001 gggaaagtgc caccacgaac agagccattt tgagaaagtg aagtatcttc ctttttgtga
     3061 acgcactctg tatcctttct ttcatcactc cacctcattt ctctgtacgg gcggtctagt
     3121 atcaaaggcg tgtagtaatt cttcaaagac tgggtgtatc tgcaccacac cgacacgccc
     3181 gtataagtcc tgcaataaac attgtccgtt ctccaaatca cgcaagcgtt tctggttgtt
     3241 ttcgtcgtcc ttgtcgatac cgaaaaattc taaggtctgc tttatctcgt tgatgtcagt
     3301 agaacgaaat gcaaacttca aaccgatatt gtttttcaga ctttcctttg acacgtcata
     3361 ggcagactgg gtaacgaaat aaacgcctgc ctgcatagct cgtccagcac gaaccagctt
     3421 atttgatagg gtttcgcctt gtgccacatt taagaacgcc cacgcttcgt ccaaatctac
     3481 aatcttaaaa atgcttctgt ccgaatggat aaaatcaagg gcaaaggtac taatcacaat
     3541 cagcatagac accgacaata attcaatggt cgtgtattcc tcaaaagtgg tatctttatc
     3601 tggcagtaca aggtctgcta cttgaatgat attgagctgg ttatcaaggc ttatagcatt
     3661 ttctaccgtc ccgtctgaaa acagcaggtg tgcaaagtcg tagtccgtga agctgtcgat
     3721 atggtctgcg atattgcgtg agatagcggt atcttctttt ctcaattcct caataacgtg
     3781 taacagtcct ctattttcac tctgggtaac agaacgcacc gcttttcgta acacggggaa
     3841 tttttcgccg tccctagagg aaatacccgt aaggaatgtt aagatgtcga ttgccagact
     3901 ttcagcgtct ttcacattct tcataatcac aaatgggtca agaagtcctg cattgtcctt
     3961 gtcgctggta aggtttacga tattgatttc atgggctatc tctgggagcg tttctttcca
     4021 gttgccacgc tcacttttag ggtctaacag aagtgcttga ccgccaaaca gcaccgcata
     4081 atatacaagt agattgttgc agaatgactt tccaccgcca agacttccca caaaagcaga
     4141 agcaagggca ttagtaactg tgccttttac tccctgcgaa gctaaagacg gttgcaggta
     4201 cacatttctt cccgtatcaa cggaatagcc catatagata cccgtgtttt cccctaactg
     4261 ctgggtcgca ccaaaaccaa gcccagccaa gaagtctgac tttacatact gcacatagtc
     4321 attgatatat cgcttgctgg caggtaaaaa ttcagaatga agtcccaaca tatcgccagc
     4381 aggacgcacc agttttacat tgaggtcgtc gtaaaagtcc ttgacttcat cacaacggcg
     4441 tttcagctcg tcaagatcgg gtgcagacac ccgtatgacg tagcttaact tatacatact
     4501 ttctttgctc tggtctaaat cggtttccag ctcgtccacg ctgtctaaag cgtccaccac
     4561 atttgagctg gtttcacttc ctgcttgata ggcgtgatta tccaaatctt tcaattcctt
     4621 tttcttgttg cggactgtcg ttaatgcctt acggttctcc acgatttcta cattcataga
     4681 agtatcaacg gggaatgtga attgctgttg ctggaaatag aagatttcag aggacggaaa
     4741 atccaactcc ccgacaatta cattgacggt aaaataggac acatagcttt ccttgtcctc
     4801 atgttccaat cgtaaatacc gctggctttc ctcaatcaca caccttgtcg gacggataag
     4861 gtcgtagtat tttatcagcg tttctttctt caatttcttc tttggtagct ggtactcata
     4921 atcttcatag gcgataccgt ccctgccgta aagatgttcc atgagatacc caaaatcatg
     4981 gatttccaag cgacgcacct taaagcgacg ggagatttta ttttccagta acttttccat
     5041 tttcatgtaa cggttgattt catcattcgg catggaaaca aaatcattca tcagcgtgtg
     5101 gttcacttca tgaagaaatt ccttgaacgt cagccatgcc gattttttga tgttcttcag
     5161 attgagctgt tcttccgtaa ccatgagctt aaagccaaga aaaaagcggt agtccacttg
     5221 attgtcccca atcattgata ctaacgcttc ggtctgttcg tctatttttt ggactgccac
     5281 ctcacgaagt ttgcctgtta ccagtttctt tgactgctcc tgcatacttc taatggagct
     5341 ttctgtggca atctgcaacg catgaatttt tccctcacgg gactgtgcga ttagctgtcg
     5401 aaaactgtca tgcacgataa atttctgctc tgcggataga aaagaatagt tgtacggtat
     5461 catctcatag taggcgaaca cctcattgtc cttgttccag acaaggttat tgtcaatata
     5521 ttttatcggg aacatagttc acactcctta ctgccgtgat tgtttcattc aatatctgct
     5581 tatgcagttt tacggctttt cctgcataag tgattttagg tcgcaaggca taggttatct
     5641 gtgatttcaa aaagctgtac ggcttctttc cgtcaaaggt tttctgcgac ataaaccaag
     5701 tgagagcaac gggaataccg aagtatttga gaaatgctcc ctcaatcatg gaaagtgggg
     5761 gcatatcccc aaacagaatg atgataaatt ccgtaatcac aaaccatgta atctgtgtaa
     5821 aggtaacggg aaagggtaag ttaaagtcat tgattgcata taagactttt tccacgttcc
     5881 agatacccgt gtagctttta atctttttca agttctttca gcccctttcg taagttcttc
     5941 aaagagaaaa ggacagccat tttcagactg tcccttaaag agaaatacgc tgtcagtagc
     6001 aacaatgctt gttgctgatc ttccagcgtt aatatggtat ctcacagata cctcggcttg
     6061 tttcaatgaa tcgaccaccc atatccaaat ctcgcccgta ggcttcatag tcaatatagt
     6121 tctgtaagtt agctggaatg tccccaagtg cgttcaattc gtctatgtag tagtaggcaa
     6181 catctgtcat ggtttcacaa tcgggataaa aataaatatc gtccttatgt tccacgactt
     6241 cttccagcgt cccatagtgg ctgataaatt cgtccagaca ctctacgata tagtcgggaa
     6301 gttcctctat catttcatac atttcattga gttcttcaat ggaaacatat tcccctatgt
     6361 cgataggaaa gttgtcggta tcatggacag cgtattcttc gtactgttca ttcaagccga
     6421 ttttttcttt tacatcttcc tcgtcaatag ggaaagtgaa ccacgcaccg actaaatatc
     6481 cctcattgta tctgccaaga ttagcaatat aaaccgccat atcatcaatc ataagcacca
     6541 cctcatttcc tattctggca gttcaaagat actgttttcc gttacaagat agcgtttgtt
     6601 ttcaagtaag tgagaagcgt aggcttcaaa atcgaaatag tatgcttgac agtcctcgga
     6661 taaatgtttg aatgtcgggt cgttcatcag cttttgcctt gcaacatcta tcatgctttt
     6721 acagtcgtga taaacggtaa tcacgttcct gcaaatataa agtgcttcta agttctcaaa
     6781 cacggtaaga agttctacat attcttcctg catatcggct gatagctggc ggtacataaa
     6841 atctaattcg ttaagctggt acacgctggt gtgttcgtgg acttcatcag cataaggcaa
     6901 caccttttcg ataatgcggt aatcttgact ttctgcaccg acacctaact tttcttcaaa
     6961 ctctgcaaca tctatcggta agtcaaacca gtatgatgtt gtttcttcgc catttgttct
     7021 tcccgtttcc accagtaccc ttgtttcctg cattgttcct cacgtccttt ctgttgtttc
     7081 atcacatctt ttaaggtctg gtatgacata ccaaacacat atttagaaaa atctttcaac
     7141 tgtttttctt catagtcggc agggttcagc cgtttcattt catgccacac cttacgcttg
     7201 taagagggca ggtcggaaat gtattcacag ccgacttttg cctgcatatc tttaaaaata
     7261 tcgttcattt catcactcca atctgaaatt taggtaagaa aaaagcagtc aatcctaaga
     7321 ctaactgctt tgtgtgtaag tatgaatttg tttaagtgta actgatttac catgtgtact
     7381 cgtaaagttt actgtatgtt tgacttttaa ataaatatat acaatcaatc atttgtaact
     7441 tgccgtcttg ttcaaattta cttattatca gattatcttc atagatatat gctaagaaag
     7501 tatcatactc tgcatagtat ttttctctaa aaagatttga ctctctaaaa tctttttcta
     7561 aagcaattct tatactgtct aaagcatatt taaagttata acaagtatta aaactaaact
     7621 tgtatggaaa aaagaaaaat aagtttttct tgaaagtaag caatttcaca taggtttgta
     7681 tatcaaattc aatcgttcca tactcatatt tctctgtaag acaagaatgt aaactttcac
     7741 aatcataatc ccgcaacgaa gcaaataacc tagttacagt catttgtgta tttggtcttc
     7801 tcggtggaca cttaccaatt acaccatcac agaatttctg tatttgacca gtaagttctt
     7861 ttgaagcatg aagtcttgtt gtgctttcaa gtaatttaaa gtccatttga taattaggtg
     7921 agttacaatc acattctcca tgattttcag atttaggtgg tacatattct gcaaaatttg
     7981 atttttctct aaaatatatt gatttattaa ctaattccaa caagtatttt tcataattac
     8041 aattttcttc atctttaata aaatttttgt ttatgaatac tgcatgtaaa atatccactc
     8101 tcatttcatc tgaaatcata agcactttta ttcctttcaa aaaataattt atcgtaccca
     8161 cattataaca ttctcatatt acagcaacaa taaaattcgt tatgctccaa taatcttatt
     8221 gaacagctct aacagtacat ctttcacacc gccagcgttg aatactaagc cgacagcaat
     8281 aagggcaact accaagaagc caataagttt tgaaaactca cgcttgaacc ctaagtagat
     8341 accgataacc acaatcgcca taagcactaa gctctgtgca tttgataaaa accattgata
     8401 caaattttgt ccgaaattca tttaattgtc ctcgctttct tctaattgtt tcatttcata
     8461 gtcagcttct ttgctgatac tcaaaataca catcaagact acgccgatac ccattcccat
     8521 agataccagc aggaagtctt ttactaattc ccacattttt tatcactcct aactttccac
     8581 caaatctttt gcaggggtcg tctgctgttt gattatcatt tcatgctttt cactcaagat
     8641 tgcctgcttt tcaatcgttt ccatgtagtc tgtaccgttt cccttatcaa tctttttcag
     8701 cattttcaag gtcggtgcta cctgtctttg tacccaacgc aatgtgcggt ctaaggtgta
     8761 aggttctggt tttgtcgtca gtttcagact ttgccgatta tcgccaataa accaagccca
     8821 gcggtcattg agtttccagt catttttccg cttgtctggt tcttcatcaa taaaccgcac
     8881 atactggttg atgatagaaa aagcggtctg ctctgcgtca tagtaggtca gcaaatctcg
     8941 tactgcatag taagcacgtt catttctaag ccgtatctca aaacggttga taatgtcggc
     9001 ttcttccaga ggtgtaccta atttgacata ctgctcatag tccttttcat agatacagaa
     9061 atacacatct gatttcagcg aaccaagata aagggtactc cccatatatt ctctgtcgtc
     9121 ctctctgtgc ttgataagct cgcccgactg gtaatactta tagcttctgg actttccgat
     9181 atattcccgt ttcctgcatt tctccgcaag ctctggaata tccaaaatgc ccgtatggtc
     9241 gttgatagca aggtcgatac gcttcataat gcctccgtct atgagtgcgt ccataagaaa
     9301 gtcataccag cttctttgct gtgccagcag gtaactttca aactgcctgc aaccccgacc
     9361 ctttaactct aaaaggacac ctttttcttc gtcagccgac gtatagataa agatgtcccc
     9421 taatgaataa tgctccgtat aactgtaatg tccgtaatct tcatggagca tataattgat
     9481 attcagtttt aatatgtctt tgatgatgtg ctgtatatcc agcgtgggaa aacgaatttt
     9541 cacataatca aacagcatgg taagcggtgc ttctggattg aaccttgcca gttctttatg
     9601 caaagtttcc agaagttctt ttgacggctt catttttccg ctttctatct tgtttagata
     9661 ttcccttgtg ataccagaag ctaccgccag cctgccttgt gagataccgt aagcaatccg
     9721 cttctcccgt aattctttta tccattgttc ttcattcaga accatacccc caatctgtca
     9781 agtgtgaagt tttttaaggc gacttcacag ccgatttttg ctaggaaacc atgccagaag
     9841 ccttatgttt cctatgtttt tttgtctatt gtctatttgc aaacgtaccc ctctgttaga
     9901 taccgagggg ttttacctgc tggcgtggct tacgccacac cagcaacggc tagtccgagc
     9961 cgtcgccttt cgcttcgcac gtcgccgtcc cgtcctgcct tgcttgtgca agaaaaccga
    10021 ttgtctgcaa aaaatcatgt cctttcggga ctaaaggcgt gtaaaattca cttatgacgc
    10081 ttgttcccac atcacaatag ccacgccctt tgatacgctt ttggaaaaac tgctttttca
    10141 catctgaacc aaacagcata ccgtaaccta actcgctgat acgccccaat cccacacgta
    10201 aattgaagtt atctctgata ccgtccgaga aatattttgc gtctggacgc tggcaggcaa
    10261 cgataaggaa ataacctgct tgtcgtccta acatgacgat tttctttaac tggctaagta
    10321 agcccacact ttcttttgtc cccagcattt caaaaaacgc cacatattca tcaaagataa
    10381 gaaagcaggg tggcagtccc agataggcgt aattttcgcc cgtcttatag ttcgggtgtc
    10441 gtttcatttg ctcgcttcgc tgtaccatgc cctcataaaa agcattgaca cagtctatca
    10501 tttcttcttt ggtgtgatac acgtttccca tgactgttcc caagtctgct aagtccgcat
    10561 ttttcgggtc taagatgtaa aggacagcgt tggtatgcag tagggcttca atgagcgtca
    10621 gaagaaaata tgttttaccg ccaccagtcc caccagcaat cagagcgtga gggagtgcgt
    10681 catattccca gacaagattt ttcatcagtc taagacagcc gttctctgcc cgtacttcat
    10741 caatggtaat gcggttcgct atcatatcat aaagcagggt atattcgata tagccgtcat
    10801 gcagggtctt gtcggtcagc tcacaataca agccactttc caatttatcc tctaaccgta
    10861 aaagctggtc ttgatatttc cccagcgtaa tttcacagcg gatatgaagc agtccctttt
    10921 ccatttgata ataaatcttt ggaaaccaga cgattttttc ccttgatctg ctttgcaggt
    10981 cagtaaaaaa aacgctgtct tgtacggtat cagcttcata ccacttgttt tccagtatca
    11041 ttcgtgccag cttttgacgg tgcagtagct ttttgaaact gtcgtaacag aagcggtaat
    11101 acagaaaagc gaccaatgca caaacaccag tcgctatcag tatggttatg aagttgtaaa
    11161 gggaaagcgt aaagccattg tctaataagc tgaaatgttc ccagtcggta cgcatgagct
    11221 gttttgtatt taatagcagg ataaccgcta cgaacacaaa caggagtgtc cccattgaaa
    11281 agcgatagac aaggttctta tcgctggctc tgatacggtg tcctttgttt aaaatcattc
    11341 tcataaatca atcactcctt tctgggtatg aaaaaagcag tcaatcgtat agactaactg
    11401 ctttgtgtgc atggatatga agttgtcaaa ctggaattta acgttcactc atatattgtt
    11461 ttatttcatc agaagtcaat tttcgtactt ctgtatgtgg atattcttta tactctccaa
    11521 cattaaacac aggtgtcatt tgtttgcatt tcttgtcttt attctttttc aagaatagat
    11581 gtaaatcctc attcccggcg aatattttat aagatactct gcctttactg ttctctatct
    11641 cataaatacc acatgaactt ttcgttgtat aatcggctgt tcgtaaataa agttttgcaa
    11701 tttccaatga cttaaaagga aaattccatc tttgtaatcc tctgtttggg tcatgttcaa
    11761 tacaaatgca acgtccacaa gttccacaaa tatattgagt gtggttttct aagcagtcca
    11821 agggatttag taatggtgtt attctatttt catcagagta acattcttca cacatttttc
    11881 ttttcgctcc ttatcaaatt ctaatttgtc gctttcttca tacccatatt ataacagttc
    11941 cgtatatcct ctacaacgaa aatctattca gcaagtctat tttttcggtt gtccctgcgg
    12001 tgtatggttc tgtgaagtgc ctgtgttctg attgccttta ttcttcagta caatatcttc
    12061 tgcctttaca taccagtcca catctgcacc cgtataggtt tttcgtgata ccgtatcggc
    12121 tacgggattg acaagctcca cgactgcatt gtaaggaaat tcccttaacg gtacttctgg
    12181 cggtacggac acggggataa ttccaccatg cagactgcac ttcaaatcat agatacgctt
    12241 tttaagctgg gtactcggtg tcccgtcctc attttgcaag aacacatcac gcaccgctgt
    12301 aaattttaat tctccaaatg ttttctcttt gtcaatgaca aatccatttg ataatctcat
    12361 aaattcttca ctcctttact tttcttcaac tgctacaata tcatcagcta ctaatacata
    12421 atcggtatgt cccacatccc cgattgcgta ccctctgccg tataacttcg gattgacaag
    12481 ttttactttc tgttcatact tgaaatgctt ttcgccagcc tgcacgggaa tttccaccac
    12541 aacattttcg cctttctgta cgtcagaata aaggttatag cttcgtctgg ctatgacctt
    12601 gcggttgttt ttatcccttt caaagatagg ctcgctttcg cctgcaaatt caagagttcc
    12661 aaaagactgt gccatatctg gcacgacata tttcatttcc atattgtttt acctcgtttc
    12721 tttctatatt tgataagttt atgattgtaa ttttttattc tggcttgtct gccgtttgac
    12781 agtttcggcg gagaacgtga tttttctttc atcataccgt taccgcctta tgattttttc
    12841 attgtacggc gtttgtaaag atatgttcca gccagtccac cagcagatgt aagcaacatc
    12901 acaagaaatg ccatgacatt tgtactgtcg cccgttttag gactgtcgct aggtctgttt
    12961 ggcttttctg gtgtctgcgg tgtttcgagt ttctctggga cttctggttt ttcattaaaa
    13021 gtaatcgtct gtccctcgtc ctcaatatcc ttatgctcgg taactttctt cggctcgtct
    13081 ggattgctta aatcatacaa ttcttcaaaa gttacaagct gtttgccgtc aagggaagta
    13141 gcgtcaaagg taaaggcaac ttccgctttc atagtttcgc tgtcagcggt aaaggtgtaa
    13201 tcactttcca cacgtttccc attgataaga agttctgcat tttcttcttt caacatctgc
    13261 cagcctacaa gtttgtactg tgtaccgact tccaagccct ctaaggttac ggtatcaatg
    13321 attgtaacgt cttttccagc ttcaatctct ttgttgccgt ctttgtcggt agcagtcgta
    13381 tgtatcttga tgatacgttc tgtaatcaat acagtttgtc cgtcgtcctc aatgtctttg
    13441 tgttccgcaa ctttcacggg ttcgtctgga ttgcttaaat cgtataattc ctcgaatgtt
    13501 accagatttt taccgccaag ctcggacgca ttgaatgtat aagaaatttc cactttcatt
    13561 tcttcatcat cagcgataaa agtatagtca ttttccacac gttttccgtc aatgataagc
    13621 tctgcatttt cttcctttaa catctgccag cctttgagct gatattttgt accttttgta
    13681 agcccgtcta atttgacagt atcgacgatt gttacctctt ttcctgcaag gatagtcttt
    13741 tcgctgtctt tgctggtcgc tgtggtatgg atagagattt ctttttcgta ttcatcagtc
    13801 aaagtcccta aatcaatcac aaggttattt cttgatacca cgatttcaaa aggtgggata
    13861 agttcaaagc ctttgttact atcgcaacgc atttcttcaa tgatgtaagt atcataaggc
    13921 aatgcacctt tgctgtcgtc tgcttcagaa gttccaaacc acacaccgtc ctcgctggtc
    13981 ttgcctgcgt tagtattgtg cttatgagaa gtccattcag cagaagtgga gaattgcccg
    14041 ttatcatcag taactacaac atgactttct cccgtcgtct tgcttgtgat cctaaaggga
    14101 acatcagcaa gacgcttgtg tgtgcctgca ccgattttca ctccctcaat atccccacgc
    14161 ttaatctgat tgtagataga gtgggcttcg tcggttaagt ccacgatttt tccattctct
    14221 gtgattgcaa agtcaatcgg cttcgcaccg tctgtcaagt aaccgtcggg agcttcactc
    14281 tcaacgatac ggaatttccc ataaggtaaa aggtcagcag aagtagaagc cacaccctca
    14341 atatctgtac gaattgtctt taccacttca tttttcttat atagcttgcc ctcaaccaaa
    14401 acagaattgt catttaagga aatgacgtca aaagcagtat ctttcaaagt ggcacttcct
    14461 tgtggtttgg tatcgctcgt ttctaaatct cttttctgaa ttttcacacc gccacggata
    14521 accttgtctg atacggaaaa ctggttactt cccgtcaata cagcaaggtc gccgtcctct
    14581 gtaatttgtg taaggtataa gccttttatc tgttcagact tatcgccagc ctgcatatat
    14641 gcaccgtcca gcaagtagcc gtttggagct tttgtttcct caacagatag tgttcctaaa
    14701 ggaagaatcg ctttgccgtc ctgcatatag aagctgtcgc cagatacctt gtaagcgtcc
    14761 gctaattttg tgatgtagtg agttgtcccg tcactgtctg tttcagcgat tgtctttgta
    14821 acccatgtac gagtagcttc ggcagggaga ttgtctttgt tatagaagcc agcataatac
    14881 ttccatgtaa actctgcacc ctctaaagaa gcgtttccct gcggattgtc tttctgcgtt
    14941 tccatatcaa tcttgaaaag ctcaatcaag gtgtccgtta cttttggtgt atctgatacg
    15001 ttcaaggtcg ctgtctttcc agcttcaact tttaaggaat acacggttgt atcaacttta
    15061 tatcctgctg gtgcggataa ttccctaata tagactgtac ctgcttttac ctctacaaca
    15121 tctgtatttc cgtttttatc agtcgtaagg gtggcaagct gtttcgtgca gtccttatca
    15181 gaaaagacac cataggtcgc accagcgatt gagtaattcc cgttaccgtc tgtaatgctg
    15241 gtattactgg aagtcttttg cagttttgca tttccgacat ttaacttcgc ccagaattgt
    15301 cccaattcct gcccctcgcc agagtagata taaccgccac attcatagcg tcctttattt
    15361 tctttcacaa aggctttagc accagcgaaa acttcgtcct gagttgcttt tggtatttca
    15421 tcataagaag ctcgcacgtt gtcgcattgc cagccaagat gtacgctcaa tctctgccag
    15481 acaacacatt gttccaacag atagacttgc ttgtagttca attccttgtg tgcttcgcca
    15541 tactgtttga catactctaa ggataacgcc acatctgaaa tctggtcggc actcatacgg
    15601 gtgcttgcgt cagctctggt cttgtagcca ttcttaaaat ctgtattgat gtctatacag
    15661 taagcgtcct cgccctcaac agtcaaatgt ccctcgttaa aggtagaacc aatcgaaccg
    15721 tcattcatta ctttttctac gataccgaca cgttcctctg attccgtcca gtattgcttg
    15781 ctttctgcat ggacggcggt actcggtaaa gcagtaacga cagtcgcaaa ggcaagtact
    15841 cccgtacaca atcgtttcaa tatctttttc ataattctaa atgctccttt cattttgggt
    15901 aaaaaaatag acgctcaata ttgaacgtct tacattgaaa agggatactt tttacaagta
    15961 cccctcaaat ctaatattta attgtttgtg ttcccacact ctatatcaac aactactgaa
    16021 acattgatat agttttttat ctctttattt ctgattttat cgccctcgct cgtaaagact
    16081 atatgcttaa aaaagtgcct aaaattaagg atttctacat agttcgtctt tgtagcaaca
    16141 cacaagtctc tatatgtgta gaatgaggga acatatctac aggttgtgcc tcaacacatt
    16201 taaatccgtg aacatttaga taatctaaat cccttgctaa agtagaagga ttacaagaaa
    16261 catagacaat tctttccgga ttcattgaaa caatagtctc taataccttt tcgtcacatc
    16321 cctttctagg tgggtctaca acgactacat tggcagtctt accctctcta tacatcttag
    16381 gtacaatttc ctctgccttt ccaacataaa attcagcatt atgtatattg tttaatttag
    16441 catttatctt agcatctttt atggcatcat ctataatttc tattccataa acctttttag
    16501 ccttttgtgc caaaaataaa gatattgtac ctatcccaca gtaaatatca aaaacagtat
    16561 cagtatcatt taaatttgca tattcaagag ccttatcata caatacttta gtttggacag
    16621 ggtttacttg gaaaaatgat aatggtgaaa tctcaaacac taagtctcct atataatcgt
    16681 ttatttttcc atctccatat ataactatat tttctcttcc tagaatagca ttcgtatctt
    16741 tttgatttac atttacaacc aaagtcttaa atccaggtat attttcctta agcactgaag
    16801 ctaattcttt aagatatgga agtttgtttc catttgctac taatacaacc attacttctt
    16861 tagttgtaaa acctattttt gttactaaat gcctaatcaa ccccgtgtga gtcttttcat
    16921 catatatgct taccttatag gcccttatat atgtttttat tattttaaca attttatcat
    16981 ttatctcatg ttgtattacg catttttctg tcgatattac atcatgagtc ttctttttat
    17041 agaagccaat aattggaata ccattattct tttgtattgg aaattgtgct ttatttctat
    17101 atctatatgg attatccatt cctatagtgt catgaacttc tacatcctct aacttaccta
    17161 ttctcgaaag tacttgttta acttcatttg ttttcatttc aagttgttta ttataatcta
    17221 agtcttgtat ctgacaccct ccacaatccc taagattgtc actacacact cttttcaccc
    17281 tgaatggaga cttttctatt atctcaacaa tatctcccac agcataattt ttctttactt
    17341 tattaatcct aactttaact ttatcatgta ttaatccgcc ctctacaaaa actgtaatac
    17401 cattgtactt tcctactcct actccgcctt gaccaatgtc tactatatct acaatataca
    17461 tctcgttttt cgaaagcata aatcaccctc caatcaatct attatattta aatctttaca
    17521 aacttttttc tttaaattta tattaaaata ttgctattaa ttaaactata ttttttacta
    17581 agctacaata ttttatttta ccaaaaaaaa aggagcttat aaagcccctt agtaaataaa
    17641 tttattattt ttcttataaa actctagttg aacctctata taccacgcct cttgatgcat
    17701 ccacagttac aacttctcca tcttttacta gatttgttat atcagctgca gacacaataa
    17761 ctggtatatc taaatttata cctacaatag ctgtatgtga tgtcattcca ccattctcag
    17821 ttactacagc tcctgctttt tctatataat tgttcatttc agcatcagta gcagtagaaa
    17881 caagtatgtc gccttcatta aagtctacac aagcatttcc attctttatt atacgaactt
    17941 taccttctac tgtttgagta cctacaccta taccttgcac gatttcttcg cttattacat
    18001 gaacctttat taaattagta gttccactta ctcctactgg tactccagca gttataacta
    18061 ctaattcccc attatctata taatcagctt gtctagctgc ctcaattgcc ttttctataa
    18121 cttcatctgt atttcctgca acagaagact taactggata tactccccat gtaagagcta
    18181 atttattcat agtcttttca ttatttgtag tagctattat tggagatttt ggtctgaatt
    18241 tagataccat tctagcagta tatcctgaag aagtagatgt tattatagct gatgcattta
    18301 aatctactgc tgttgtacaa gttgcatggc ttattgcatc tgttacagta acattgttag
    18361 taccattttc ctttagtaat ctatcataat ctaaagtctc ttctgttctt tttgctattg
    18421 tagccatcat ttttactgcc tctacaggat attttccagc agcagtttct ccagataaca
    18481 ttatcgcatc tgttccatca tatatagcat tggcaacgtc tgtaacttct gctcttgttg
    18541 gtcttggatt tcttatcata gaatcaagca tttgagtggc agtaacaact ggctttgcta
    18601 attcattaca tttttttatc atcatttttt gaactattgg catctcttca gttggtatct
    18661 caacacctaa gtcacctcta gccaccatta taccatcaga aacttttaat atttcatcta
    18721 ggttctctac accttcttgg ttttctattt tagatattat ttgtatatca gttgcattat
    18781 tgttctctaa tacttctctt attgctagta catcagatgc ctttcttaca aaagaagcag
    18841 ctatataatc tattccttga cttattccaa actctatatc acttatatct ttaggagtta
    18901 ttgctggtaa atttatctta acaccaggta catttactcc tttatgattt ttaactattc
    18961 ctgaattttc aacaacacaa actatatctt ctccatttat ttcttttact cttaagccaa
    19021 ctaaaccatc atctattagt atagaatctc ctgatttaac gtcttcagca agacctttat
    19081 agcttactgt acacatttct ttagtcccca taatatcttt catagttata gtgaactttt
    19141 gcccttcttc taaaaatacc tctgggtctt caaagttacc tgttctaatt tctggacctt
    19201 tagtatctaa taatatagca actggcttat ttaatttttc tctaactttc tttgccatat
    19261 caattctttc tttatgttct tcatgcgaac cgtgagaaaa gttaaatcta cacacattta
    19321 gaccattttg cataagttga gttaaaactt cctcactttg tgatgctgga cctagtgtac
    19381 aaacaatttt tgtcttcttt acattattta acatatttgc tcctcctgag atattattcc
    19441 acagatttat ttttaatact aaaacttata tagataatat ttttgccatc tcataagctt
    19501 ctttatcaaa tactttcttt tgagctaaag cttcacttat ttctaaatca attattttgt
    19561 tttcttttat tcctacaact ctagctgatt ttccatctaa taataattct actgctctta
    19621 ctccaagtct acttgctagt attctatcag atacagttgg gcttcctcct ctttggacat
    19681 gccccaatac agtgactctt aaatctgctc ctgactcttt tttaagttcc ttttctaaat
    19741 cactagcact tccgactcct tctgctaaaa cgattatact atgtctctta cctctttttt
    19801 gagtagtctt taatcttaga gcaacttcat ctactgtaat ttctacttct ggtactatta
    19861 tagtttccgc tcctcctgca agacctgcat ataaagctaa atctccacag tgtcttccca
    19921 taacttctac tatgttaact ctttcatgag atgatgatgt atctcttatc ttacctatag
    19981 catctataat agtattcatt gcagtatcaa atcctatagt ataatctgta tatgctaaat
    20041 cgttatctat agttcctggt atgcctatag ctgggaatcc taagtcacta agtttttggg
    20101 ctcctgcaaa tgaaccatct ccaccaatta caactaagca atcaatttta tactttttaa
    20161 gaatttttac tgcttttaat ctaccttctt ctgttttaaa ttcttcacat ctggaagatt
    20221 ttaatatagt tcctcccctg tgtataatat ctccaacaga agataagttc atttcagtta
    20281 aatcttcttc tagtaatccc ttatatcctc tattgattcc atatactttg catccataat
    20341 atattgctga tcttacaacc gctctaatcg cagcattcat tcctggagca tctcctccac
    20401 ttgttagtaa tcctatagtt ttcatttttt acctccttgg attttatgtc aattttttat
    20461 tataatcatt gttattgtat tctaatggac tttttttgtc cctatatcac tttttaatcc
    20521 catcaagact aaaaaaatta aatgtcctta aatgtccatt ttgtgatatt atatcacaaa
    20581 acggacaata tttttcatat tattataata tatttagatt tttatataaa aaatcaagct
    20641 tttactttta tgttatcttt aggaagtaac tcttctaagt tatttataaa ttcatcacat
    20701 atacaagtca atgccccggt cattttatat gctttacgtt taccatttat attttcatcc
    20761 atagggtata agtatatact gtcattacct ggatattgtt tggctatttc tacaatatta
    20821 ttagttgtat cagtatcaga catactatct attttcaaat acaatttttt accactttct
    20881 ggcttaatat tttctacatt tctagttctt cctgaattgt agtttctatc tgcaaagtca
    20941 gtatcatcat ttatatcttt tatctctcta gctataagtt ttgcattttc gccttctttt
    21001 atacttaatg ttccttttac atatattata ttatcttcat tcaaaatgac attatacttt
    21061 tgtaataatt gaggaaaaac tattatttct atagcaccat acaaatcttc taattcaata
    21121 aatgccataa tttcatttcg ttttgttgtt ttaatagttt tagttacaat cattccaccc
    21181 attataacat ctgtttcatt catttgtagg tatgactctt cttcctcttt taatgaattt
    21241 aattttccat tatccataga tgttttttct tgtagttctt tttcaaattg tgataaagga
    21301 tgtccactta catacattcc caagacttct ttttcaaggt taagtctctc tctttcttcg
    21361 tactcttgcc ttttaggcaa actgtatatt tgttgaaaat cactatcttc ctcactatct
    21421 ccaaaagcat caaataaaga aatttgacct tgaacatttt tctttctatc catagataca
    21481 ctttctaata aactttcata accagccatc agactagctc tattttcact tatattatca
    21541 aaagctccac attttattag actttctatt actctcttat ttgtatcttt ttggtctaat
    21601 cttttgacta aatctactaa atctacaaat aatccattag attctctttc agcgattata
    21661 ttgtttatta tatttacgcc aacatttttt acggctgcta gaccaaatct tatattgtcg
    21721 ccttcaactg aaaactttga aaaacttttg tttatatctg gaggtaatac atctatttct
    21781 agtgctttac attctcttat atactcaact accttatcag tatttcccat tacactggtt
    21841 atcaatgctg ccataaattc aactggataa tatgttttta gataagctgt ctcatatgct
    21901 aaaaccccat atgcagctgc atgtgattta ttaaatgcat atctagcgaa gtctatcata
    21961 tcatcaaata ttttattagc tatttcctct tctacaccat ttctgataca ccctgcaatt
    22021 tctatattac catcttcatc aaatttacca tgaataaaat attgtctttc ttcttccatc
    22081 acgtccatct ttttcttact catggctctt ctaactaggt cacttcgtcc gtaactataa
    22141 ccacctaaat ctctaacgac ctgcataacc tgctcttgat ataccaaaca accatatgta
    22201 acttccatta taggctttaa tttttcatgt agatatgtta catcttttgg gttgctttta
    22261 ttttttatat atgcaggtat agaatccatt ggacctggtc taaataggga tattcccgct
    22321 acaatatctt caaaattgtc tggttttagc tgtttcataa aacttctcat gccagcactc
    22381 tccaattgaa acacccctag tgtatttcct gatgaaagca tttcatatac tttcgggtcg
    22441 tcataatcca tctttgaaaa gtctattttt tctgtgtaat tttttataac tctatttttt
    22501 tctattaaat caagagtatc tcttataact gtaagagttc ttagcccaag aaaatccatc
    22561 tttaaaagcc caagttcttc aagtgttgtc atagtaaact gtgttgtgat tgcatcttga
    22621 tgtttgtaca aaggtacata ttcatctacg ggattttttg atataacaac tcctgctgca
    22681 tgagtagaag catgtcttag cattccctct atctgttttg atatatttat tatttctttt
    22741 gtttccgggt cttggtcata caattcttta agattaggat ttgtatctag ggccttatct
    22801 attgtcattc ctaaagcaaa tggtatttcc ttggctacct tgtcaacttt attgtagcct
    22861 atatccaata cccttccaac atctcttatt gcagcttttg cacccattgt tccaaaagtt
    22921 attatctggg ctacatggtc atcaccatat tttctcttta cataatctat aacttcttct
    22981 cttctctcat aacaaaaatc tatatctata tctggcatgg atatacgttc tggatttaaa
    23041 aaacgctcaa ataatagaga gtactttatt gggtctatat cagttatttt aagtgtatat
    23101 gctactatcg aaccagccgc actacctcta cctggtccta ctataatatt attttcttta
    23161 gaaaaattta taaaatccca aacaattaaa aaatactcta cataacccat tttttcaatt
    23221 acatctaatt cataatttaa tctctctagt atctcattac taggattatc atatctctct
    23281 tttaatccat taaaacacaa ctcccttaaa taagtttctg gtgtatatcc ttctggaaca
    23341 tcatacttag gaagatgtat agtattaaaa tcaaactcca cattacacat atcagctatt
    23401 ttcacagtat tgtcaatagc ctctaatgca tatggaaata attcttccat ttcttctctt
    23461 gattttaaat aaaactcatc actaccaaat ctcattctat ttgggtcatt taatgttttt
    23521 cccatttgaa tacacataag tacatcatgt attttcgaat cttctttatt tacatagtga
    23581 acatcattag tagcaactag tggtatacca gtctcttttg ataattttac taaaccagag
    23641 tttacttctt tttgctcagg taaattgtga tcttgaatct ccaaaaagaa attttcttct
    23701 ccaaatatgt ctctgtagtc aagagcaaat tgttttgctt tttcataatt tctattcatt
    23761 aaagcacgtg ctacatctcc tgcaaggcat gcagaaagtg ctataatccc tttgttatgt
    23821 tttttaagtt cttccatgtc aactctaggc ttatagtaaa aaccttctac gtatgaagtt
    23881 gaaactattt ttattaaatt tttatatcct tccatattct tagctaacag aactaaatgt
    23941 ccttgatatt tgtcatagtt tgggtcttta tctctcaatc cacgtgcagc tgtatagact
    24001 tcacacccta taattggctt tattccttct tttatagctg ttttgtagaa atctataaca
    24061 ccaaacatac aaccatggtc agttatagct attgagctca tgtttagttc cttagccctt
    24121 tttataagtt ttttcaccct cgaaaaccca tcaagtaaac tatactcagt atgtacatga
    24181 agatgagcaa aatctttgtt cattaaatca cctcctgctt tataatttta attttaccac
    24241 aaatatatgt cctaaaacaa aaacagagac ttttatctca aaataggaat taacttcaac
    24301 atataccatt atatgttata aattaatgct tattctgaga ttgtcccctc aatttattgc
    24361 tatgattttt ttttcaaatc tgttgcaatt tcttctattt ttctaagtct atgattgact
    24421 ccagattttc ctattggtgg ttctaacatc tctccaagtt ctttgagagt catatcctca
    24481 tactcgattc taatcttagc tatctcttgt aaattttctg gcaaactgct aattcctata
    24541 gtctcttgta tgagcttaat attttctact tgtcttactg ctgcatttac tgttttagaa
    24601 aggttagctg tctcacaatt tactatcctg tttacattat ttctcatttc ttttactatt
    24661 ttagtatttt gaagacttaa aagagcgtgg tgtcctccta taatactcaa caaatctgat
    24721 atttgttcac tctctttgat ataaacaacg tgattatttt ttctagccac aattttacta
    24781 ttaaaaccta aagagtttat aagttctttt agagaatctg caaactcctc attattagtt
    24841 acaaattcca tatgatagtt cttttctggg tcgcttatag aacctccacc aagaaaagct
    24901 cctcttatga aagctctctt acatccttca tgcttaatta agttctctga aattttgttt
    24961 atagtaaaaa atccttcttt atcttcaagt atacctaact tttttaataa catttctgaa
    25021 cccatttcgc ttgttaccat taatacataa ctgttatttc tctttagcat ttgattctta
    25081 tttactgaga ttgtagtatt gatattaaaa ttctttttta ataatttaaa tactttacgg
    25141 gctattgaat ttaactctgt tgttatcttt agatttagct tcttatatcc aacaatttgt
    25201 atacttcccc ctgatttaac tagagctgac aattcagcta tattacaaca ttcattccca
    25261 gatataactc ttgctagttc atttttggtt tcagttgaaa aagacatatc tatcacctat
    25321 taactaattt ttctttaacc ttttttttat ttcttgtaag gcaaccttta ttatctcctt
    25381 ttcatcatca tacctagcta agtctactac tctatctagg tggataaatt tagcatcaat
    25441 aaagccttcc tctttttgtg gaatagtatc catattcttt gcttgcatca aataccaaaa
    25501 aactgtttta tgaactgctc tattttcatc ccagttttct ttgaatgtat agtgtatttc
    25561 acctaaatat ttcaatatat ctgcttttac tccagtttct tcacttactt cacgtaatgc
    25621 tgcctcttgg ttattttccc cttcttctac ttttcccttg ggtaacaccc aatctccatt
    25681 gaacttcctt agtaaaagaa tcgtattgcc aaacaggact acaccaccag cactaacctc
    25741 ttctctcatc gatgttcact ccttaaaact aacataatga taaggatttc tacacaattt
    25801 tttaaaatcc ttttttacta acttttgttg taattatgat ttagggctaa ctcaataact
    25861 atgttagata tatactttgc atcatgtctt atataattat tttttatttc aattaaattc
    25921 ttctctacag tttttatacc catctctttt aatttatctt tttgcttttt atctaacaag
    25981 acttgctctg ctccatcttg tttatataag tcaagcattt cttctggcaa tatctcatta
    26041 tttgcgatta cataatctat taaattctca tcagtgtgtt ttactatagc attaacatgc
    26101 tctaatacat tgtacccttc tgtctcacct ggttgagtca ttatatttgc tatatatact
    26161 tttggagcta cagacgattt aatagcgtct acaatcccct ctacaagtaa attgggtatt
    26221 atacttgtat ataaacttcc aggacccata attataatgt cagcatcata aatagatgca
    26281 ataacatcct ttaatggttt tacatctttt ggatttaaaa aaattttatc aatagagctt
    26341 ttttgaattt ttgactcttc aggtatacta gattctccat ttattatatt gccattttct
    26401 aatttggcaa ctaaatttac atcttccaaa gttactggta aaactcttcc tgttatagca
    26461 aaaatttcac ttaatttata tacagctttt tcaaagtttc catacaaccc attcatagca
    26521 gccaaaaaca aatttccaaa actctgacct tttaaaagcc cttctgtaaa cctatattgc
    26581 ataacttcat tcattgttgg ctctatattt gcaagtgcta ataaacagtt tcttatgtct
    26641 cctggtggga gcattcctaa gtcttctctc aatacacctg aacctccacc atcatcagca
    26701 acagttacaa ttgcagttat attttgcgta gtgtgtttaa gacctcttaa aaataccgat
    26761 tgaccagtac ctccacctat cacaaccaca tttggttctc tattttcaac aatagacctt
    26821 tgaaatagtt ttactttttc attttttgct tttttatata taaataacga tacaattaat
    26881 gcaatccaac ctaaaattcc tacaataata agtaatttag ccatgtttgc ctccattatc
    26941 gcaacataga gtctctatgt ttctttacaa ctctatatcc cttattagat aaatcctccg
    27001 ctataagatt tgttatagta actgacctat gtcttccacc tgtgcatcca acaccaatta
    27061 ctagatgttg ttttccttcc tctatatatt gaggtactaa aaaatgaatc atatctaaaa
    27121 gtttcacata aaattcttca ctgattttag aattcataac ataatctcta acttctttat
    27181 catcaccagt tttagctctt aactcttcaa cataataggg atttggaaga aatcttacat
    27241 caaatactaa atctgcatca gcaagtatac catgtttaaa tccaaaagat actacagaaa
    27301 tagttaggtt tggattatct tcacctgaag aatatatttt ctttatttct tccttaaggt
    27361 ctttaggttt catgttagtt gtatcaatta tacatgtaga taactccttt aaaggttcca
    27421 ttatctttct ttccattttt ataccttctg gaatctgcat atcttttgcc aatggatgat
    27481 ttcttcttgt cattttatat ctctttaaca atacatcatc attacaatcc aaatatacca
    27541 tttcatattc ataattttct ttttctaaat aatttaagct ttcatgaagc gcttcaaaaa
    27601 actttctccc tcttatatct atccctagag caactttatc aatacttgaa tttggttgat
    27661 aacataattc tgcaaattta gttatcaaag caggaggtag attatctaca caataaaatc
    27721 ccatatcctc taaagctctc attgtctcac tttttccaga tcctgaaaga cctgtaacta
    27781 taacaaattt cattgctaac tcctcccttt tatcttatgt ttattatatt ataatgtttt
    27841 ctactaacga tatatccaga ttagagtcag atatagtctt catagctagt tcaaaatcac
    27901 caactctttc tggacaatgt gcatctgagc ctattataaa tttattttct agccctttta
    27961 ttttatttaa ttgatttaca ttcaaaaaag aatggctact atttatctca agtctagtat
    28021 ttgtcttttt tgctgtcttt gctacttctt ctatatacac atcgccttta tctcctggat
    28081 gagtaattat aaaaatatca ttattactta tcgcatttat tacagctttt gtattatatt
    28141 cttttgcttt ttcagtttta aaaatatagt tattacaatg atttaataag cttcttaatg
    28201 atgttggagt agaaccaaaa tgatatccag ccataatata atcaaaatct ctaataaatt
    28261 tatcatccat atctatattt cctttatcat ctaatatgtt acactccata cctaaaagta
    28321 tatttatatc agtgtactta tcatttaaat aatctacttc ttctctcatt ttgagaatat
    28381 cttttttctt tacaccatat gcaatatgtt tataactatg gtcagatatt cctattgttt
    28441 ttattccttt agaaatggct acctttacat tatcctcaat agtccctttg ccatgactat
    28501 ataatgtatg tgtatgataa tcagccagta ttttcatttg ttaatcctct ccaagtattt
    28561 tgacttcttc ttccaacata accccaaact tggcatatac agcacttttt ataacatata
    28621 ttaaatccaa aatatcttta gcacttgctt ctccttgatt gactacaaat ccacaatgct
    28681 tctcagatac ttgtgctcct cttaaagtaa gacctctcaa tccagtttct tctattaatt
    28741 tacctgcaaa atatcctgtt ggtctcttaa atgtacttcc tgcactagca aaatttaatg
    28801 gctgtttagt tattcttctc gtcgctaaat catccattaa actttttatt tcatcataat
    28861 ttccttcttg taattccaat tctgctgata aagctatata tccgtttttc gatattatac
    28921 tttttctata cccaaattcc atctgttcat ttgataattc aaatatattg ccttccatat
    28981 ccatcagtct tactgacttc actatgtcct tcatttcacc accatatgct ccagcattca
    29041 tggctaatgc tcctcccaat gtgccaggta ttcctgatgc aaactcaaac ccttttaatt
    29101 cttgcttttg gagagctttc cctaaaactg ataataaagc tcctgactga gcagtcatcc
    29161 tagtatcatt tatctcataa gaattgaaat tatcagctag ttctattacc actcctctta
    29221 ttccaccatc tttaattaaa atgtttgaac catttccaat tattaaatac ggaatagatt
    29281 cttcttttac caacttaagt acatttttta attgttcttc agttctaggt ctaactaata
    29341 tatctgctgg tcctcctacc ctaaatgaaa tatgtttttt cataggttca tcgactttta
    29401 tatcctcttt acttaatata tttaataaat ttatatatat atcttgatta ttcattgtat
    29461 ttcctcccaa attatactga actttagctg tcattattat ttaaattaca ttagtatata
    29521 gtattaacta tattcaataa tatatgattt tgcttatcat atggatttta tattttaagc
    29581 ttcttaatta caaactatat tcaataaata tataataaag taaaaaaaaa aaaataacaa
    29641 catataaaag aatataaata atttaaaaaa agcatcaata tcactgatat caatgcttta
    29701 aaaggcaatt taattcgtat tatttatcta actatttttg acatagcaaa gataataaat
    29761 ccctccaact actatggaac ctccaacgat atttccaagc gttacaaata ccaaattagt
    29821 tataaattga accatagtta tatttgctcc taatgccatt cccattggta taaaaaacat
    29881 gtttgcaaca ctatgttgga atccacataa tacaaatagc attattactg tccaacatga
    29941 agccaatttt gacattatat cttgagatgc tgtagccatc catacagcta aaacgactag
    30001 aatattacaa agtattgctt ttaaaaacat tgatgctata gttgaattaa ccttagcttc
    30061 tgccacagct atagcttttg tagctgcatc tcctgtcaat gtagaagaat aaaacacaag
    30121 tgcaactagc aaaattgaac ctactaaatt tgcaaaataa accagtcccc agtttcttaa
    30181 catttttcct aatgttattt ttttgtccat aagtgcaata gccattaagg tgtttccagt
    30241 gaataattct gctcctgcca ctactacaag cataagacct actggaaaga ccatagcact
    30301 taaaaagcta gctacccctg ggtctatctt gccaaaagtt tgactaacta tcatactccc
    30361 caatgcccca aaacctataa atgctcctcc aaataaacct aatagaattg cgttttgtgt
    30421 cgaaatattt gcttttttaa tgcctgcact tatagttgct tgtgcagttt ccccaggtgt
    30481 caagaatcct ttactcataa atccttcccc cttaaaaatt atactttccc ttgtcttttt
    30541 atggcaataa ttatataata acaatatgat atataataaa ttttattaac ataaaaaata
    30601 tactatatac ttatatatac ttgtctcata taattatact atttattata taacaacttt
    30661 gttaatttcg aaataattta tattgaattt gaaaaattta ccttttgatt atttatttta
    30721 ctttttaatt attgaggtat tatatataat agatatatgt ttgtgttatt atatataatt
    30781 acttatttta ataaatattt gaaggaggga attttatgtt tgatatatca tttttcgaaa
    30841 tgtcaatata tgaaatagca gttactacaa tatatataat taatttcatg gttatactaa
    30901 accttatctt tagagaaaaa agaaacataa atacaacttt aacatggctt cttatattag
    30961 tattgattcc tgctcttgga ttcatacttt atatagcttt tggcagaaat atttcaaaaa
    31021 acaatatgtt tagattgaaa gaaaaggatg ataagataat caaaagtaat atactagata
    31081 ctcaggttaa actccaatct acatctgaaa ttgattcaga tatacatcaa cataaggata
    31141 tgatttatgc attggctaat tcaaataatg ctcattatac caataataac gatgtgtgga
    31201 tttatgctga atctagtcaa ttttttaaca gtcttcttga agaacttaaa aaagctaaaa
    31261 aatatataaa catacaattt tacattttca aagatgacaa aatcggaact gaaataatag
    31321 acattcttat agacaaagct aaagaaggtg ttgaagtaag acttttattt gatgcagttg
    31381 gtggtagaac actaaaaaat agtacacttt ccagacttaa agaaagtggt gttaaagttg
    31441 gaagcttctt cccatcattc ttgaaaattg taaactttaa cttaaactat agaaatcacc
    31501 gaaaaattgt tgtcattgat ggtaaggtag gatttgtagg tggatataat gttggtgatg
    31561 aatatttagg tagaaatcca aaatttggtc tatggagaga tacacatact aagcttactg
    31621 gtgattgcgt aattgaccta aatatgagat tcatccttga ttggagatat acaactaaag
    31681 aagacttaga tttagaaaag tactttatag aagaagatac aaattctaat catccttctg
    31741 aaaatattgg gattcagata gtatcaagtg gtccagatat atcagaactt gatgagatta
    31801 aatatggtta ccttaaaatg attcagaaag ctagaaagta tatatacata cagagtcctt
    31861 atttaatact tgactcaact tttatagata ctttaaaaat agcttgttta tctggtgttg
    31921 atgtgagagt tatgattcct tctaaacctg accatccctt tgtatactgg gcatcatatt
    31981 catatgctgg agaactttta aagtttggag ctaaaatcta tacttatggt caaaatgcat
    32041 ttttacatgc taagacaata gttatagatg actctatatg ctctatagga acagctaata
    32101 tggatatcag aagttttgag cttaatttcg aagttaatgc atttatgtac tcttcaaaaa
    32161 aagcattgga acaaagaatt atatttgaaa atgatatttt aaattctaag gaaataacat
    32221 tagatatata taattctagg tctacttata taaaaatcaa ggaatcaatt tcaagactat
    32281 tatcagcagt tttataaatc ttttatttta taaaaatttt acattttggt ttatttttta
    32341 aagtttttta tatgtattat ttttttttta ttgaatttag ctcatttttt ttattttttt
    32401 taactattct gaattatttt atattataat agactagtat agtttttatt tttaataggt
    32461 aacttttttg cattgttttt ataaataaag aaattgttgt atattattct ttctttattg
    32521 ttttggtttt gttcacccac ataatgttgg ggtcaactca tggttaatta ttttatgaaa
    32581 aataataact ttaaaaagaa aagggggatt tgttatgtgc gattttattt cacacaacaa
    32641 gcaattattt gacgaactag atatatttat tgactctctt gcaacaaagg aaggtgcgtt
    32701 aatacaagtt ctacatgaag cccaaggtat atttggatat cttccaaaag aagttcaact
    32761 tcatgttgct agaaaacttg gagtagctcc tgcaaaggta tatggggttg ttacattcta
    32821 ttcttatttt actacagaac ctgtaggtaa gtataaaatc agtgtctgtc taggaactgt
    32881 atgttttgta aaaggtgctg acaaaatctt aagtgctttt gaaaaacagc ttggcataaa
    32941 agttggagaa actactagtg attttaaatt ttctttagaa ggtttaagat gtttgggggc
    33001 atgtggttta gctccagttg ttactgtaaa tggtaaagtt tatggtaaag tgaaacctga
    33061 ccaagtaagc gaaatattag atacttatag agaattagaa ttgaattgtt aagaggggtg
    33121 aataaataat gcgtaaagta aattcatttg atgaattaaa agttttagct gatgagctga
    33181 aacctaatct tagcttaaga aaaaattatt ctaaggaaaa tgtattaaga agagaattac
    33241 ttgtttgttg tgatactgga tgtacatctt caaacagtct agaaatagtt agtgaattag
    33301 aaaatgaaat aaagaaatca ggaatccaag ataaagttag tgttcgtttg acaggttgtt
    33361 ttggtttctg tgctcaaggt cctattgtaa aagtttatcc tgataacgtt ttctacgtaa
    33421 aagttgagcc atctgatgct gaaaagatag ttcaaagcca cttaattaga aatacagttg
    33481 tcgaggaatt attatatgag gaagaatctt taagtaaaaa agttgaagaa caagaagaaa
    33541 tgtctttcta caaaaaacag ttacgtatag ctttaaaaaa ttgtggtctt atagacccag
    33601 atagtataga agaatatatt gccaatgatg gttatttagc tctaggcaaa tgtttaactt
    33661 ctcttactcc tcaagaagtt attgcagagg taaaaacttc tggccttcgt ggtagaggtg
    33721 gtgcaggttt ccctactggc tctaaatggg aagctgcttc aaaaaatcca gctggtccaa
    33781 cagataaaaa gtttgttgta tgtaatgctg atgaaggtga cccaggtgca tttatggata
    33841 gatctgtact tgaaggtgac cctcatagtg tactagaagc tatggcaata tgtggatacg
    33901 ctataggttc tgatacagga tacatataca taagagcaga atatccaaaa tctatagaaa
    33961 gattaaaagt agccatagct caagctgaaa aatacggttt acttggcgaa aatatcttag
    34021 gcactggatt taactttaaa ttagaattaa aatatggtgc aggtgctttc gtttgtggtg
    34081 aaggtacagc gctaatgcat tcaattgaag gtagacgtgg tgaacctaga atgaaaactt
    34141 atagttcttc taagagtggt ctatggaaat ctcctacttg cttaaataac gttgagactt
    34201 ttgctaacat acctgcaatt atacttaagg gtggagattg gtttgccaac ttaggtactg
    34261 aagattctag tggtactaaa gtctttgctc ttggtggaaa agtagaaaat gtaggacttg
    34321 ttgaagttcc aatgggtact actttaagag atattgtatt tgaaattggt gggggaatcc
    34381 ctgaaggtaa gaaatttaag gctgttcaaa caggtggacc atctggcgga tgtataccaa
    34441 ctgaacattt agacactcct atagactttg gttcattaag ttctatcggt tcaatgatgg
    34501 gctctggtgg tatgcttgtt cttgatgaat ctgactgtat ggtagatata gccaagttct
    34561 tccttgagtt tactgttgaa gaatcttgtg gtaaatgtac accttgtcgt ataggtacta
    34621 caagattatt agaaatatta actaaaatta cagaaggtaa tggtactcta caagatttag
    34681 atgacctaga aaaccttgct gaaactatac aaactgcttc attatgtggt cttggtaaag
    34741 ctgctccaaa ccctgtttta agtactcttc aatatttcaa agatgagtat atagctcatg
    34801 ttgtagataa gaaatgtcct gctggtaaat gtcagaactt actttcttac tttataacag
    34861 atgactgtaa gggttgtaca aaatgttcta gagtgtgtcc tgctggttgt ataacaggtt
    34921 ctgttaagga acaacataca atagatactt ctaagtgtct aaaatgtggt gcttgtatag
    34981 ataattgtac atttaacgct ataatcaaga aataacttat ttggaggagg aataacaatg
    35041 agtttagtta atttaactat taatggcaaa cacgtttcag cacctagtgg aacttctatc
    35101 ttagatgctg cgaaattaat aaatataaaa atacacaacc tttgtcattt acatatgaat
    35161 gaaatagata agcttgacac ttgtgcttct tgtcgtgttt gtatggttga gacagagaga
    35221 ggattagttc cagcttgtgg aacagttata aaggaaggta tgaaagttca gactaatagt
    35281 gctaaagcat taaatgctcg tagaactatt gttgaattat tgttatcaga ccatccacaa
    35341 gattgttttg tctgtgaaaa aaatggagat tgtgaattac agactatagc tgctgattta
    35401 ggagtaagaa aaataagata tcaaggttct aaatcttttg caggtaaaga tacttctact
    35461 aaatctttag ttaaagacca ttctaagtgt atattatgta gaagatgtga gactgtatgt
    35521 aatgacatac aaacagtagg agcattatct ggagttaatc gtggatttaa tactttagtt
    35581 agtacattct ttaactctga tatggttgaa acagagtgta cattctgtgg tcaatgtata
    35641 tctgtctgtc caactggagc attaacagaa gtagataatg tacctaaact ttgggatgtt
    35701 ttaaataaaa aagaaaagac tatagtagtg caagttgctc ctgctgtaag agttgctatt
    35761 ggtgaagagt ttggattaga accagggtca atctctacag ggaaaatggt agctgcttta
    35821 aaagcacttg gatttgaaca tgtatttgat acaaactttg gtgcagattt tactataatg
    35881 gaagaagcta ctgaatttat agaaagaatc caaaaaggtg aaaatcttcc tatattaaca
    35941 agttgttgtc ctgcatgggt aaacttctta gagcacaatt atccagacaa gttaaactta
    36001 gcatctagct gtaagtctcc tcaaggtatg tttggttcta tagctaaaaa ctactatgca
    36061 cctaaaatat taggaatcaa tcctgaagaa ctttatgttg tatctgtaat gccttgtgta
    36121 gctaaaaaat atgaagcttc aagagaagaa ttaagtgaat ctggaatact agatgttgac
    36181 ctttctataa ctactagaga gttagctaag atgataaaag aagctgcaat cgatttacct
    36241 aatcttcaag accaagattt tgataatcct ctaggaaaat ctacaggtgc tgcatctata
    36301 tttggagcaa gtggtggagt tttagaagct gccctacgta cttcatatga gaagataact
    36361 aataagactt tagataatgt aaacttcact aatgttagag gtcttaaagg aattagggaa
    36421 gcaagtatag atgttgatgg aactacagtt aatgtgtgta ttgtaaatac acttaaaaac
    36481 gctagaaaga ttatggataa ggtcagaagt ggagaatgta aataccatat catagaagtt
    36541 atggcatgtc ctggtggatg tgttggaggt gctggtcaac cttatcacca tggtaatact
    36601 gaaatagttg atagacgtgc aaatgcgtta tatgaaatag atagaaataa ggctattcgt
    36661 aaatctcatg agaatcctga tttacaagct atttataaag acttctttgg agaaccaaat
    36721 agtgatgtag cccataaata ccttcatact cattactttg ataagagttg tgtctatgga
    36781 gagtgtccac aagaatgtgc ttgtgaagaa gctaagtagt cataacaaaa tataaaattt
    36841 attcttaaaa aaggattgtc ttaaaatact tttacgtaat ttaagacaat ccttattaat
    36901 ttcacaaatt aaattatatt tctaaataga cttattcttt aattaaccta tctattaatt
    36961 aaagaatacc tgccattctc ataagttgtt ttgcatttgt agttttacat cttccatctt
    37021 ttatcttata gtcaaaatga attttatcgt ctacatagta ctctgagaaa tgatagttta
    37081 caacttcaac atctccacta cctgaaaggt cacatagctc aaagtcatgt gttgtcacca
    37141 tacttataac ccagggtttt gataatttct ttactgcctc acttgctcca ataattctat
    37201 cagcagagtt agttccctta aaaatttcat ctactagaac caacattggc agttctttta
    37261 aactatattg aatcattgat tttattctaa gaatctcagc atagaaagtt gatattcctt
    37321 gacttacatc atcttgtatt ctcatagaag taaatatagc catacaggtc gcatcaaaac
    37381 tcttagcgca tactggagca cctgcatatg ccagcactaa gtttacaccg atacttctta
    37441 aaaatgttgt ctttccagac atatttgaac ctgtgataat acaagtctga gaatttaatt
    37501 gtatcgaatt tgctatgaca cttttttctg ctattaatgg gtggtatacc tcttcaactt
    37561 tcaatcttgg agtatcttca tattttatta ctggaaaaca tgtattttcc tttacatgag
    37621 aaacaactgt taaacttatt agagcttcaa attctccaat agattcaaac caatctctaa
    37681 tttgatttcc atatatacct ttccatcgct ccaaagcttc tgcacaatga tagttccaca
    37741 tcaaaacacc acaggcaatt atatatagta tctgattata ccttaggttt acagcatttc
    37801 ctattgaatt taattgttta acacctcttg aaactccact tcccttagaa agtttatctt
    37861 gtaattcttt caaatatgca ctctcaaacg tctctttttc taaaacctca aacatttttt
    37921 catatacttg tatacttcta ctaaatgaaa ataatggagc aagagtttgc ataagtcttg
    37981 gatatccaaa accactaaac cctaactgga ttataaaaag taaaaaaaca ggtaaaacag
    38041 gtaaaaatcc taacatacaa aatataaaag ataagattgt tgcagttggt aaaccccaag
    38101 taaaaatatg catccatttt ggtatataca cattcttatt ttctccatat tctataaacg
    38161 attctatact actatctgaa ttgttttttt gctctttacc aattatatta ctgagtgttt
    38221 gaatatgaat agaaaaatca tcttttgata aaagttcttt tatagctctc tgtctttcta
    38281 agattacatt ctcatctgga ttttcattcc acaaatattt ggctaaaaaa tttttcccat
    38341 aagatgtatt agctacacag atatattgat acaatgatgc tcttccaaat aagtctaaat
    38401 cttttgcttg cgaattttca tcttttaagt attctttacc atctatttta aattccttcc
    38461 aaccatctcc aaatctctta atatacttat ttaatacttc gctcttactt ttaaaatatg
    38521 tttcttcttc ttttacttta ctatgaataa ctaagagtgc tataaatatt attattgata
    38581 aaactccaac aaaaatacct actatatttt tattgtaaaa tccaatagca aaccctgcta
    38641 tcactatcaa aaaagatatt aatcttgctg tacttatgtt atttgacttt ttacttaaga
    38701 gcaattcttt ttcagtacta ctttctaaaa gtttactaaa atattctatt cccataaaaa
    38761 attccaactt tcttgttata taatgaaact caaatataaa attaatctag tattattata
    38821 atctaacact gtactttaaa ttatccttat ataatatatc ttattaataa acattatata
    38881 tatacaactt gattttaatt tataaggtgt aggacataat cctaatcaat atttaattaa
    38941 atgatgtgta gttcactatt attcagtact acttagtatc gattaatttt gataattttt
    39001 tagtaaattc ttgagctgca tcgtatccca ttcctctttg tctaaaattc attgctgcta
    39061 cttctaatat catagcagta tttcttccag gtttaacagg tacaactaat ttctctacat
    39121 taacaccaag tatttcttca tattctttat ccagtcccag tctatcataa tacttagttt
    39181 catcccaagc ttctaaatga actactaaat ctatcatttt agaatttttg attgccccta
    39241 ctccatataa acttctaaca tctattattc ctattcctct tatctcaaga aaatgtctta
    39301 ttagttcagg tgactgaccc acaagcatac tttcatctaa ttttcttatt tctacagcat
    39361 catctgcaac taatctatta cccctttgaa tcaattctaa tgctgtttcc gactttccta
    39421 tactactttc accttttatc agtacaccta taccataaat atctactagc accccgtgaa
    39481 ttgttgtgtg tggtgctaac ttattatcca aatagtttga cagtctattt ataagtctag
    39541 tggtatttct atttgtgctc aaaagtattc ttttatattt tttagccttt tcaattacat
    39601 ctggcttgat ttccaagtct ctagaaatta ttaacgctgg tatttcatat gagaaaaatc
    39661 tatctagtac ttcttcccga atatgactat ccattaatcc aaaaaaatta tattctgtct
    39721 ttccaactat ttgtattctt tcatatgcaa aataatcaaa atatcctgca aactgtaatc
    39781 ctggtctatt tatatcttga gagtagacat aataatctac atcatcaggc atgtatacta
    39841 cttttaactt caactctttt attatctctc ttattgatac tttattgcta gtttccatta
    39901 attttcttcc tttctaaaaa aattataaac attatctgca atgcttttat tcattccatc
    39961 tacttctaaa agttcttcca tagtagcttt ttttatagca tctacatctt taaagtgatt
    40021 taataatgat ttctttcttt tttcacctat tccttgtata tcatctaaga tagattttgt
    40081 caaagcttta tttcttagac ttctatgata tgttattgca taattatgaa cttcttcttg
    40141 tatactagca ataaatctat ataaattagt tgttttatct agttctattt ctttttcttt
    40201 gcatatcaat cctttagttc tatgcttatc atctttgtac attccccaaa gaggtatatc
    40261 tacatcattg agttctagta ctttttttac tgcactaact tgtccttttc caccatccaa
    40321 caatatcaag tcaggtaaat ttccatgttt taatcttcta tctacaattt cagccataga
    40381 atcataatca ttaggtccaa taactgtctt tattttatat ctcctatatt ccttcttatc
    40441 ttttttagca ttcgtataca ctaccataga accaatcgaa tctacacctt gtatatttga
    40501 tatatcataa gcctctattc ttattggcaa cttttctaga tttaatattt gctttaattc
    40561 ttccaatgct ccaatacttt tttcatattt tcttttattc atatcagaaa atttttcaag
    40621 atattctaca gcatttttcc taaccatttc aactaagctt ttcttctctc ccttttgagg
    40681 aactcttatt gttacttttt gccccttttt agatgacagc cattcttcta atatagaact
    40741 atcttctatc tcatcttcta taattaattc tttaggtata tactcttgtt cattataaaa
    40801 ttgttttaca aatgaactta atatagaagc tcttggacta tccattaccc cttctaatat
    40861 aaaatgttcc ctacctacaa tcttaccatt tcttataaaa aatacctgga cacaagcttc
    40921 attatgagct cttgccattg caaccacatc ttggtttaag tcacttacag tagcatctat
    40981 tttttgtttc tgtatcatct cttcaaggct ctttatctta tccctatata cagcagcctc
    41041 ttcaaatcta aagttcatag aactctcatt cattttttcc tttaaaagtt ccatcaattt
    41101 ttcttcttta ccagataaac acattataat ctcttctatc atttttccat actcttcttt
    41161 agaaacattt cctgtacaag gtcctaaaca tttatttatg tgtagattta aacaaggtct
    41221 tgtcttattc tttattgctt tatctatatc tattttacag cttcttattg gatacgtact
    41281 acttataagc tccaaggtat cattcacagc agttatattg gtatagggcc caaaatactt
    41341 tgccttatct ttaagaactc ttctaacttt taagattctt ggataatctt cattcgtagt
    41401 tacttttata tatggataag ttttatcatc tctaagcact acattatact ttggtctgta
    41461 tctttttatt aaattacatt caagtattaa tgcctctaac tctgaatccg ttataatata
    41521 ctcaaactta tatatattct taaccataga ttttacttta gatgtatggt tttttgaaga
    41581 ttgaaaatac tgccttaccc tattttttag agatatggct tttcccacat atattatatg
    41641 gtcgtatttg tctttcatta aatatactcc gggctcagaa ggtaattttt ttaaatgctc
    41701 ttgtatatca aacatctcgc cctccaaaac attaaatttt atactaaaat tataccctaa
    41761 aatatgaatt ttaaaaagtt atacaatttc cagtgcttta aacaagttaa agcccatatt
    41821 cccttctata attgatttga aaacaatttt aaccatgaag agtaatatgg actttacttt
    41881 gtattactat ttacaactta tatttgacta atttacttat aacaaaacag tttatcgtat
    41941 atctgctcta aaatttttca caatttatat aacttaagaa aaatatttct ttaagaattg
    42001 acctgtataa gaaccatcaa ctttagatac ttcttttggt gttccagttg caactatagt
    42061 tccaccctta tctccgcctt ctggtcctaa gtctattaca taatcacaag tctttataac
    42121 atctaaatta tgttctatga ctataattgt atttcctgta tctgctagtc tttgtaatac
    42181 ttggattagc ttgtctacat ctgccatatg aagtccagta gtaggttcat ctaatatata
    42241 caaagtcttt cctgttggtc ttttactaag ttcagcagct aattttatac gttgtgcttc
    42301 tcctcctgat aattgagttg aaggctgtcc taatttaata tatgaaagtc ctacatccat
    42361 taaagtttct aattttcttt ttatatttgg tatattttcg aaaaactcaa cagcttcttc
    42421 cacattcata tctaatatat cagatatagt tttatctttg tattttactt gcaatgtttc
    42481 tctattgtat ctttcacctt tacaaatttc acaaggaaca tatacatctg gtaagaaatg
    42541 catttctatt tttattattc catcaccctt acaagcttca catctaccac cttttacatt
    42601 aaaactaaat ctaccttttt tataacctct tgctttagct tcattagtag atgcaaaaat
    42661 atctcttata aaatcaaata cacctgtata agtagcagga tttgaacgag gtgttcttcc
    42721 aataggactt tggtctatat ttataacttt atctatactt tcaattcctt ttatctcctt
    42781 atgcttacca gctctttgct taagtctatt cactttactt gcaacacctt tgtaaagtat
    42841 atcgtttatt aatgtacttt ttccagaacc tgaaacacct gttatgcatg taaacttacc
    42901 taatgggaac tttacatcaa tatttttcag attattttct gatgctttaa ctatttctat
    42961 aaaacttcca tttccttctc tagtagtctc aggaagcaat atttgttttt taccacttaa
    43021 atattgaccc gttattgagt tctcattttc aattatttca tctaaagtac cctgtgcaac
    43081 tatttcacca ccatgtatac ctgcacctgg accaatgtct ataacataat cagcttctct
    43141 cattgtgtct tcatcatgct ctacaacgat taaggtattt cctaaatctg ttagatgtct
    43201 aagagtagtt ataagcctat cattatctct ttggtgaagc cctatacttg gctcatcaag
    43261 tacatataaa actccaacca atgcagaacc tatttgagta gctaatctta tacgttgcgc
    43321 ttctcctcct gataaagttc cagcttttct tgataaattt aaataatcaa gacctacatc
    43381 tcttaagaac atagctcttc cttttatctc ttttataatc tcatgagcta taaatctctg
    43441 tttttctgta agatttatat tttccataaa gtcaatcaat tcatttacag ataagtttgt
    43501 aacttccatt atattttttc catcaactaa aacagataaa acttcctttt taagtctcat
    43561 ccctttacac tttggacatg gtctctctgc catgtattct tcaatcttat ctctcatgta
    43621 atctgaatta gtttctctat atcttctctc taagtttact attacacctt caaagtatga
    43681 tttataattt ctagttcctc caaattttga ctcataagta aactctacaa ttatattatc
    43741 tttaccataa agtaattcct ccacaaaatc atctggtaaa tctttaaacg gtgttgtcaa
    43801 agatacatta aaatgctctg ctaaactttt tatcatttta ctgtaataag tgtcatcatt
    43861 aacacctgtt gaaccccatg ctgccactgc accttgtttt attgataaat ctttgtttgg
    43921 tactactaat tctgggtcaa cttctttact ttccccaaga ccattacaaa catcacaagc
    43981 accaaaggga gcattaaatg aaaacattct tggagataat tcttctatgc caattccatg
    44041 ttctgggcat gcaaatttag ttgaaaaaag aatttcttct ccatctatta tttgggctat
    44101 tacaagacca tcagatagct taactgcagt ttcaattgaa tctgctagtc ttgattctat
    44161 tccatcttta attactattc tgtccactac aacttctata ttatgttttt gatttttatt
    44221 taagattata tcatcactaa cttcatagtt ttcaccatta actctaactc ttacaaatcc
    44281 ttcttttttg atattatcaa agactttttt atgtgtacct ttttgtcctc taacaattgg
    44341 tgaaagaatt tgtaatttcg ttctatctgg aaaatctagc attttatcaa ctatttcttg
    44401 aatagtcatc tgacttattt tttcaccaca tgtagggcaa tacacatctc ctactcttgc
    44461 aaataatagt ctcaaataat catatatttc tgttactgtt ccaaccgtag aacgtgggtt
    44521 ttttgaggta gtcttctggt caattgagat agctggtgat aaaccctcta tatattctac
    44581 atttggcttt tccatctgac ctaaaaattg tcttgcataa gcagatagac tttctacata
    44641 tcttctctgt ccttctgcat atattgtatc aaatgccaaa gaagacttcc cagaccctga
    44701 caaacctgta aaaacaataa atttatctct tggtaattct aaatctatat tttttaaatt
    44761 atgttctttt gctcctctta ttataatttt atcttccata atgttgtttt tacctttcta
    44821 tttttccaat ttttcttcta attgtttcac tttatctcta agttctgctg ctctttcaaa
    44881 ttgtaagttt tgagcagctt ccatcatctc tgattttaat ttatctatat tagctcttat
    44941 ttcgtcttta tcatctgtat cagatattcc atatacaact tcttcctctg caaccttagt
    45001 agcttctata ctatctctta tatttttctg tatagtttta ggaatgatat tgtgctcttc
    45061 attatataaa ttttgtatat ctcttcttct ctttgtttca tcaatagcat tttgcattga
    45121 gtcagttatc ctgtctgcat acataattac tcttccattt tcatttctag ctgcacgtcc
    45181 aatagtctgt attaaagctg tttcggaacg taagaaacct tctttgtctg catctaaaat
    45241 tgctataagt gaaacttctg gtatatccaa tccttctcta agtaagttta ttcctacaag
    45301 gacatcaaac ttccctaatc ttaaatctct tattatttct gttctttcta atgtaactat
    45361 atctgagtgt aaatatttaa cttttatccc tatttcttta agatagttag tcaagtcctc
    45421 actcattttt ttagttaaag tggttataag cactctttca tttttttcta tagttttagt
    45481 tatttctcct accaaatcat ctatttgatt atttataggt cttacttcta caattgggtc
    45541 taaaaggcca gttggtctta ttatctgttc tgctattgtc tctgagtgtt gaatttcata
    45601 aggccctggt gtagcactta caaacaacat ttgatttata tttccttcaa attcttgaaa
    45661 atttaatggt ctattgtcaa gtgcagaagg taatctaaaa ccattctcta taagtgatgt
    45721 ttttctagac ctgtctcctg catacatacc tcttacttgt ggtatagtga catgtgcctc
    45781 atctacaatt atcaaataat catctgggaa gaaatccatt aaagtatatg gtctttctcc
    45841 ttctgacctt cctgtaatat gtctagaata gttctctatc ccctgacaaa atcctatttc
    45901 ctttaacatt tctatatcat acttagttct ttgttcaatt ctttgtgctt ctaatagcct
    45961 atcattttcc ttgaaaaatt taatttgttc ttgaagctcg ttttctattt ctacaattgc
    46021 cttttcaatt ctttctggcg ttgttacata gtgagatgct gggaatataa caacatgatt
    46081 tctagtacca acaatcttcc ctgttacata atcaatttct gttattctat ctacctcatc
    46141 accaaaaaat tctattctta ttgctttttc atcattacta gctgggaata tttctagtat
    46201 atctcctcta actctaaagg tacctcgtgt aaagtttatg tcatttcttt cgtattgtat
    46261 ctctattaac ctttttatta catcatctct gtctctttgc atacctggtc ttatagataa
    46321 cataagttct ttatagtctt ttgggtcacc aagtccatat atacaagata cagatgatat
    46381 tattatagta tctcttctct ctaaaataga tgctgtagca gaatgtcgaa gtttatctat
    46441 ttcatcattt atgcttgcat ctttttctat ataagtgtca ctgtgagcta catatgcttc
    46501 tggttgataa taatcatagt aacttacaaa gtattcaact gcattgtctg ggaaaaattc
    46561 tttaaactca ctgtaaagtt gtgctgctag agttttatta tgagccatta ttagggtagg
    46621 tttcttaact tgttgaatga tatttgccat tgtgaatgtt tttcctgaac ccgttacacc
    46681 taataatgtc gaaaatttct catttctatt tatagaatcc actattgatt taatagcttc
    46741 tggttggtca cctgtaggtt tgaagtctga ttttatttta aaatccatag tatcactcct
    46801 tttttcgaac attagttcct gacatgtatt attatatcat acctttataa aattgtttaa
    46861 gttattttaa atattttacc catttaaatt ttacttaaat ctaaaacata aaaaaagact
    46921 tctatctttg caatagaagt ctataattca tggaaaataa atactttatt caacttacat
    46981 aataaaatca aatgcttctt tttcaactcc atctaatttt aaattaaaca tttttaatat
    47041 agttgctgca tgtgctctca ggtctccttc ttctataacc acaccacctt taatatttgg
    47101 tccaaaagct ataaatggag gtctattacc tttagtaggt aaatgaccat gtgtcgcaac
    47161 agcaaacttg taatctttta tatctgaatc ttttataata cttccttcga aatcatttcc
    47221 aaatgaaatt ccttcaagac cttcaattac catttcaaaa tcaccttcaa gaccaaggtt
    47281 agatgcttcc tctttggtaa aaatattctc tatgaataat tcttctttaa attcttcaag
    47341 cagttctctt aattctgtta acctatctaa gttatttact attatttgag ctgatattcc
    47401 agaagaattt acataggcat caaaatcctt aacttctcca ttttcaactt ttataaatcc
    47461 atttgattta agcaagacat ttggatttat aactttatca actctaagat gaccatggtc
    47521 cccaagtatt ataaaatttg tatctttgta tgtacctgca tcctttgtag cctgaataat
    47581 atctcctaac catttatcat tcatttttaa tgctttatct acttcgtcat taaataagcc
    47641 ataattgtgt cgtgtatggt ccaatgtagc taaatgtatt agcattaaat ctggtttata
    47701 ttcctttatt atatctactg aacagtttac tccaaataaa tccatgttgg gttctaattt
    47761 ccaatcgata taatgacaat gtctattgta aataccgtcc attataacct tactggaact
    47821 tcgttcaaaa gtttctcttg ggtcatcttc tctactagga gcccatattt ctgcaatatt
    47881 ataatctata tttggatttg ctcccattac aggccataat acactagagg tagttagatt
    47941 attttctttt gctacatcta taatagtttt tgcttttatg tcctttgagt accagtacca
    48001 gtctttattt tctttatttg ggtcaaattt ttcattatga tatataccat gttttttagg
    48061 atatactcct gtaaccatac tagtatgaca cggatatgtc agagtgggat aaacacaatt
    48121 tatatttttt actatggagc agttttctag tatagattta aagtttggta gattttttac
    48181 aaattccaag tctttttcaa ataaagaatc tactgataat actataacct ttgtcattta
    48241 cattacctct tcatttatat attattgaat acttttaatc aacttacaag tctatataat
    48301 tatttatttg ttttatctat tactgcttgc attgcagaag ttcctttgct tactacatct
    48361 tttggatttg catttaaatc agtaagcccc tttttaatct catccattag cttatcagtt
    48421 gcttccttgt atccatttaa cattggaagt gcaacagcat attgtaattg gtcagttgca
    48481 accttatatt gaggtttttc tttatataat gtttgtaact tttctgagtt tgttgcatct
    48541 tcagttgttg gtaagtatcc tgttgatgaa ctaaactgaa cgattttatc tgtttgtgtc
    48601 atccattcta taaattctgc acttgcttct ttttctttat ctgttccacc ttcaagcata
    48661 actagattag ctccacctgt aggagttgcg tatttttgat tttttggtaa gaatgctgtt
    48721 gatatttcaa acttaccttc tgtttgttgc ataagtcctg ctaaatctgc tgttgatgta
    48781 aatatcatac ctactttacc atttacaaaa tcttgttttg ctacgtccca ggcattataa
    48841 tcttctccag gaggcatttt cattattcca gctttcatca tatcttgcca gaacttaact
    48901 ggtgcttgtc ctgcctcatt attaaatgct acttttttac cattagttat ttctcctcca
    48961 ctttgcatta ccatagcttc atagaaccaa atatctattg gtgtttcaaa accataagta
    49021 tcttctgatt tatttgtcat tttactagca taatccttaa gctcttccca tgattttgga
    49081 cctgaagggt caagacctgc tttagttgcc atatctttat ttatgtataa tattggtgta
    49141 cttctattaa atggcatacc aacaaactta tcctcataat atgaatttgt taaaagacca
    49201 gaaaagaatc tttcttgata tgttttatca aaatatttac tcatatctac taaagcacct
    49261 gctgatgcga atgtacctgt ttgagttatt tctaataatg ttacatctgg ttggtcttta
    49321 gatgttaagg ctgcctgtag ttttgttgca ttttcatagt aatctttctg ttttaacata
    49381 tttacttgta catccttatg ttcactatta aattggtcaa ccattttttg agcagtttct
    49441 ccattagttc cacctaaagg agcccagaaa tctatcttcg taatattgct atcagtcttt
    49501 tctcctttag atgcatttgc atcattagaa tttgaacacc caactaaaga acctaataat
    49561 aatgttattg ctaatcctgt tgctaatttt cttttaagct tcattttcat cctccaaatt
    49621 tagtctttaa ttttttattt tattcctgaa tacataaaag cttcttttac ttttttattc
    49681 gcaaatatat aaacaagaat tattggtaaa attagaatta tatttcctgc cattattaca
    49741 ttccatgcag taattccttc tgaagattta aggagcgcaa ctcctattgg taaagttctt
    49801 attgcatctg tatttgtcat tactaagggc cagaaataat tattccaatg gtatataaaa
    49861 ttaaataata taaatgttat tactgctggt tttgccatag gaaccattat tctaaacatt
    49921 atttttaagt cagaagcatt atctagttta gctgcttcaa taatttcatc tgaaatctgc
    49981 ataaaattct gtctaagcag aaatatccca aatgaagatg tcataaatgg aattataagt
    50041 ggaacataag tatttataaa tccaaactta ctcatcatca tatagactgg tagaaaagtt
    50101 acttgtggtg gaatcataag tcctacaagc aaaagcccaa atagcatttt ttcaccttta
    50161 aatttaatct tagaaaatgc atatgctgct ggaaccataa ctaaaaattg tacaattaaa
    50221 acacttcctg ttacaaatac actgtttagc aaatatctaa aaaatggacc tgaatgccat
    50281 gctgtactat aattttctaa cactatatca tttggtataa tagtgggcgg taattgcatc
    50341 atttcttgtg gatttttaaa ggatgtagat agcatccata taaaagggaa tataaatatt
    50401 gcagataaaa gtaatagaat taatatttct attgattttg atgctatttt ttttatatca
    50461 actttgtctt ttttgatagg ttgtataata tccattgtct cctccttttg gataaacctt
    50521 ataatatcta ctttttcttt attgataatg aactttcttt gataagagtc taaagttagc
    50581 atatgtagca atcataacta tagctaacaa aaacatactc gctgcactcg cttgccctat
    50641 gtcatagtat ataaatccag actcatatat ataataagat aacatgttag ttgaacccac
    50701 tggcccacct tgtgtcatta tacttatctc atcaaatact tgaaaagatg cagttgttgt
    50761 tgttatcata agaaaaaata ttgttggtga taatagtggc aatgtcactt taaagaaatt
    50821 cgttattttg tttgcattat ctaatttaga tgcttcatat atatagtttg gtatggattt
    50881 aagacctgat aacactatta agatattata tccaagagtc ttccatattc ctattagaat
    50941 tagtgacatt aaagctgaac tttcacttga aagccacttt gatgttggta aatgtaatgt
    51001 cgatagtatc cagtttaaaa agccatattg tggctccata atccaaagcc acaatacagc
    51061 tacagatact agtgaaatta catgtggtgt aaagattata ctttgcgtta aattgtgtat
    51121 tatagaattt ttatttagcc aaacagcaag tactatggct agtgatatag atatagtcac
    51181 cataaaaaaa gtataaattg cagtgtttgt tatagtttgt ataaactctg gattctttaa
    51241 aagttctgta taattgttta agcctacatt ttcaaaggtt gaactaagaa gactaccaga
    51301 tttgaagctt agagatatcg tcttaattat tggatagact ataaacaata aagttcctat
    51361 aatagcaggt gttatatata aatatgcttt attaaattgc tttttcataa atatctcctt
    51421 taattttaga atacatactt ttacatactt ttacatcttc tattctttct ccatttttat
    51481 caaaataata taaatctttt tctaatacac ttattcttaa agttcttcca atatcaaata
    51541 tatcattttc tgttttgact cttatctctc tattaagaac agataaacaa tatatagttt
    51601 catttcccaa cacttcttta gttacaactt ttccaaatag actaaataca tcatcatttt
    51661 ttccatctat gtttatactc tctagtgttg ctttttctgg tctgaatcca aatttaatat
    51721 cattttttat atctagtatg ttcatacaag gactccctat aaattgagct acaaataaat
    51781 ttttaggatt ttgatatatc ttctttggag aacctatttg cattatcttt cctttgttca
    51841 ttataactat cttatctgcc attgacattg cctcaacctg gtcatgagtt acataaacaa
    51901 atgtagactt tagtttttga tgtaattgta ttaattctgt tctcatttga tttcttaatt
    51961 tagcatctaa atttgacaat ggttcatcca ttaaaaatac ctttggattt ttaaccatag
    52021 ctctagcaag tgccactctt tgtctttgac ctccagataa gttgcctggt ttcacattta
    52081 aatattctct caatcctact acatcaataa cttctgttat aagtttttct cttttttctt
    52141 tttctacttt tttgttttta agaccaaatt ctatgttttc tctaacagtc atatgtggat
    52201 ataatgcata attttgaaat accatagcta tatccctttt actagcatcc gtttttgtaa
    52261 catcattttc ttcaatcttc aacctacctg aagttatatc ttcaagacct gcaatcattc
    52321 ttaacgtagt agatttacca catccacttg ggcctaccaa tactgtaaaa gaaccatctt
    52381 ctatagtaag atttaaattt tctataactg tatttttttt attataagat tttgttacat
    52441 tttctagtct tatatctgcc atttttatcc tcccaaaata tttaatttaa ttctacgatt
    52501 tacttataga ttaataatac tcttaatttg taaaggatat attaagacaa agtgaatata
    52561 tggtaaactt attaaaataa aggttaaatt ataaacattt tttgttaaat atactgaggt
    52621 taagatgata tacagcttat gttagctata attttctata tttttcatat tcattgtttg
    52681 ctatcacaat gtaattttaa tttatgaaga gtcatataaa actatttatt ttttatataa
    52741 tttgatttga aagtaaaaaa gacctctatg ggggatagag gtctttaagt gggggagatt
    52801 gagtaattta tttttgctat attaagtttt acaattatat tatgtcccaa atttacaagg
    52861 tttaaacata aaaaataaaa attttttaat ttttataaat ttacccaaaa aaaattacgt
    52921 ggctacgtcc tactctccca ggcagtttcc caccaagtac catcagcgct agagagctta
    52981 acttctgtgt tcggaatggg aacaggtgta tcctctctgc tattgtaacc acataatctt
    53041 tatgcttaat atttc
//
LOCUS       DAMJWU010000015        52390 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMJWU010000015 DAMJWU010000000
VERSION     DAMJWU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.015"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..606
                     /locus_tag="PC460_001051"
     CDS             <1..606
                     /locus_tag="PC460_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2599947.1"
                     /translation="ISGYLTKKLQDLNVDTIRTDILTSPTVTDVIVWNIEQSGTDTFS
                     ATYEVDQQIKEGEQTTTVKATYTVKVHVDADRDMVIIQNPTLAPAIEKSDYEPKTPEA
                     DASVDADTVNDATAFLETFFKLYPTATAKELAYYVFGNVLEPIGRDYLYSELVNPVLT
                     KDGDNMKVKVAVKFLDNQTKATQVLQYELILHKDSNWKIIM"
     gene            819..1676
                     /locus_tag="PC460_001052"
     CDS             819..1676
                     /locus_tag="PC460_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2599948.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVTSLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            1754..2335
                     /locus_tag="PC460_001053"
     CDS             1754..2335
                     /locus_tag="PC460_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2599949.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            2439..3284
                     /locus_tag="PC460_001054"
     CDS             2439..3284
                     /locus_tag="PC460_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2599950.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            3281..3964
                     /locus_tag="PC460_001055"
     CDS             3281..3964
                     /locus_tag="PC460_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2599951.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIICFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVEHM
                     WKSLGGVKL"
     gene            3961..4659
                     /locus_tag="PC460_001056"
     CDS             3961..4659
                     /locus_tag="PC460_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2599952.1"
                     /translation="MKKILSSTIQIFKQIKSDPMMFAACFSPFIMGTLIKFGIPFLER
                     ITKFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            4671..5582
                     /locus_tag="PC460_001057"
     CDS             4671..5582
                     /locus_tag="PC460_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2599953.1"
                     /translation="MEKGRLLQNKNMNKAIQVSKITLIINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVVSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALNIFKESINKMVDHS
                     CDNATETRIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            5634..5774
                     /locus_tag="PC460_001058"
     CDS             5634..5774
                     /locus_tag="PC460_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599954.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            6065..6691
                     /locus_tag="PC460_001059"
     CDS             6065..6691
                     /locus_tag="PC460_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2599955.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            6813..7529
                     /locus_tag="PC460_001060"
     CDS             6813..7529
                     /locus_tag="PC460_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2599956.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            7679..8275
                     /locus_tag="PC460_001061"
     CDS             7679..8275
                     /locus_tag="PC460_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDF2599957.1"
                     /translation="MKILWIIIIMADNLLPLLLARFYPNYKHKEMALSVLGSRQSPVK
                     WIYNVWCIISGVVFCIAPYALYQENDSGWAIAIWMLLAIYGIGCEIISGVCPLNENRQ
                     DEDIITKIHGGTSAIGFMALLVVPLLFAILQFQTTKVVLGAISLVCFTIAFIFFCFFI
                     MGEKEKFANTVLRYGGLWQRLVLISCYIPLFVWSVSNQ"
     gene            8362..8553
                     /locus_tag="PC460_001062"
     CDS             8362..8553
                     /locus_tag="PC460_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDF2599958.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            8577..8807
                     /locus_tag="PC460_001063"
     CDS             8577..8807
                     /locus_tag="PC460_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599959.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            9074..9493
                     /locus_tag="PC460_001064"
     CDS             9074..9493
                     /locus_tag="PC460_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2599960.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            9496..9750
                     /locus_tag="PC460_001065"
     CDS             9496..9750
                     /locus_tag="PC460_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2599961.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            10125..10328
                     /locus_tag="PC460_001066"
     CDS             10125..10328
                     /locus_tag="PC460_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2599962.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            10406..11596
                     /locus_tag="PC460_001067"
     CDS             10406..11596
                     /locus_tag="PC460_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2599963.1"
                     /translation="MSKMKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPTGKRDDKSLREKEKQIKKDLDDGIDTIGGKMTVCQLYAKKNNQKKNIKRN
                     TEIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFHFKLSDVLEDDTEPKVILTPEQEEKLLSFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHE
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            complement(11853..12653)
                     /locus_tag="PC460_001068"
     CDS             complement(11853..12653)
                     /locus_tag="PC460_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2599964.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(12848..14446)
                     /locus_tag="PC460_001069"
     CDS             complement(12848..14446)
                     /locus_tag="PC460_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2599965.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            14774..14950
                     /locus_tag="PC460_001070"
     CDS             14774..14950
                     /locus_tag="PC460_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599966.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            15124..16254
                     /locus_tag="PC460_001071"
     CDS             15124..16254
                     /locus_tag="PC460_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2599967.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            16495..16845
                     /locus_tag="PC460_001072"
     CDS             16495..16845
                     /locus_tag="PC460_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2599968.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            16853..17302
                     /locus_tag="PC460_001073"
     CDS             16853..17302
                     /locus_tag="PC460_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF2599969.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            17404..17979
                     /locus_tag="PC460_001074"
     CDS             17404..17979
                     /locus_tag="PC460_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2599970.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            17972..19381
                     /locus_tag="PC460_001075"
     CDS             17972..19381
                     /locus_tag="PC460_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF2599971.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            19565..20998
                     /gene="eutA"
                     /locus_tag="PC460_001076"
     CDS             19565..20998
                     /gene="eutA"
                     /locus_tag="PC460_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF2599972.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            21016..22380
                     /locus_tag="PC460_001077"
     CDS             21016..22380
                     /locus_tag="PC460_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF2599973.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            22393..23274
                     /gene="eutC"
                     /locus_tag="PC460_001078"
     CDS             22393..23274
                     /gene="eutC"
                     /locus_tag="PC460_001078"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF2599974.1"
                     /translation="MNEKDLKALVEQLVGQMLGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            23294..23947
                     /gene="eutL"
                     /locus_tag="PC460_001079"
     CDS             23294..23947
                     /gene="eutL"
                     /locus_tag="PC460_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF2599975.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            23958..24659
                     /locus_tag="PC460_001080"
     CDS             23958..24659
                     /locus_tag="PC460_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2599976.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            24662..26131
                     /locus_tag="PC460_001081"
     CDS             24662..26131
                     /locus_tag="PC460_001081"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF2599977.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            26227..26514
                     /locus_tag="PC460_001082"
     CDS             26227..26514
                     /locus_tag="PC460_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2599978.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGATAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            26641..27402
                     /locus_tag="PC460_001083"
     CDS             26641..27402
                     /locus_tag="PC460_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF2599979.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            27415..28044
                     /locus_tag="PC460_001084"
     CDS             27415..28044
                     /locus_tag="PC460_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2599980.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            28086..28811
                     /locus_tag="PC460_001085"
     CDS             28086..28811
                     /locus_tag="PC460_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF2599981.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDGFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMNGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDILNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            28824..29096
                     /locus_tag="PC460_001086"
     CDS             28824..29096
                     /locus_tag="PC460_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF2599982.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            29089..29640
                     /locus_tag="PC460_001087"
     CDS             29089..29640
                     /locus_tag="PC460_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2599983.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKICAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            29652..30743
                     /gene="eutH"
                     /locus_tag="PC460_001088"
     CDS             29652..30743
                     /gene="eutH"
                     /locus_tag="PC460_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2599984.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            30745..31218
                     /locus_tag="PC460_001089"
     CDS             30745..31218
                     /locus_tag="PC460_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF2599985.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            31731..32252
                     /locus_tag="PC460_001090"
     CDS             31731..32252
                     /locus_tag="PC460_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2599986.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            32631..33806
                     /locus_tag="PC460_001091"
     CDS             32631..33806
                     /locus_tag="PC460_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2599987.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDILKLGCEFMAKYAVEFLNKNNN"
     gene            33840..35378
                     /locus_tag="PC460_001092"
     CDS             33840..35378
                     /locus_tag="PC460_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2599988.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            35410..36612
                     /locus_tag="PC460_001093"
     CDS             35410..36612
                     /locus_tag="PC460_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2599989.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(36779..37378)
                     /locus_tag="PC460_001094"
     CDS             complement(36779..37378)
                     /locus_tag="PC460_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2599990.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            37548..38906
                     /locus_tag="PC460_001095"
     CDS             37548..38906
                     /locus_tag="PC460_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599991.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            38899..39531
                     /locus_tag="PC460_001096"
     CDS             38899..39531
                     /locus_tag="PC460_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2599992.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            39860..41017
                     /locus_tag="PC460_001097"
     CDS             39860..41017
                     /locus_tag="PC460_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2599993.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTLDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFILIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(41164..41799)
                     /gene="lexA"
                     /locus_tag="PC460_001098"
     CDS             complement(41164..41799)
                     /gene="lexA"
                     /locus_tag="PC460_001098"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF2599994.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(41903..43030)
                     /locus_tag="PC460_001099"
     CDS             complement(41903..43030)
                     /locus_tag="PC460_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2599995.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(43351..44196)
                     /locus_tag="PC460_001100"
     CDS             complement(43351..44196)
                     /locus_tag="PC460_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2599996.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(44226..44597)
                     /locus_tag="PC460_001101"
     CDS             complement(44226..44597)
                     /locus_tag="PC460_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2599997.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(44750..45010)
                     /locus_tag="PC460_001102"
     CDS             complement(44750..45010)
                     /locus_tag="PC460_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF2599998.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(45199..46143)
                     /locus_tag="PC460_001103"
     CDS             complement(45199..46143)
                     /locus_tag="PC460_001103"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF2599999.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(46164..47018)
                     /locus_tag="PC460_001104"
     CDS             complement(46164..47018)
                     /locus_tag="PC460_001104"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF2600000.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKLLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(47033..48394)
                     /gene="accC"
                     /locus_tag="PC460_001105"
     CDS             complement(47033..48394)
                     /gene="accC"
                     /locus_tag="PC460_001105"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF2600001.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(48410..48862)
                     /gene="accB"
                     /locus_tag="PC460_001106"
     CDS             complement(48410..48862)
                     /gene="accB"
                     /locus_tag="PC460_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF2600002.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(49132..49578)
                     /locus_tag="PC460_001107"
     CDS             complement(49132..49578)
                     /locus_tag="PC460_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600003.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            49853..50131
                     /locus_tag="PC460_001108"
     CDS             49853..50131
                     /locus_tag="PC460_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600004.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(50260..51006)
                     /locus_tag="PC460_001109"
     CDS             complement(50260..51006)
                     /locus_tag="PC460_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600005.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEI
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(51135..51779)
                     /gene="trmB"
                     /locus_tag="PC460_001110"
     CDS             complement(51135..51779)
                     /gene="trmB"
                     /locus_tag="PC460_001110"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF2600006.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(51917..52237)
                     /locus_tag="PC460_001111"
     CDS             complement(51917..52237)
                     /locus_tag="PC460_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF2600007.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT        19391 a         7232 c         8680 g        17087 t
ORIGIN      
        1 atcagcggtt atctaacaaa gaaattgcag gacttgaatg ttgatactat tagaaccgat
       61 atactgacta gccccacagt tacagatgta attgtgtgga atatcgaaca gtcgggaacg
      121 gacacttttt ctgctaccta cgaagtagat cagcagataa aagagggaga acagacaaca
      181 acagtcaagg caacctatac ggtaaaagtc catgtagacg ctgacaggga tatggtaatt
      241 attcagaacc ctacccttgc accagcaatc gaaaaatcgg actatgagcc aaagacacca
      301 gaagcagacg caagtgtaga cgctgatacc gtgaatgacg ctaccgcatt tctggaaaca
      361 ttctttaagc tgtacccgac agccacagca aaagagcttg cctattatgt atttggaaat
      421 gtgcttgaac ctatcggcag ggactacctt tattcagaat tggtaaaccc agtacttaca
      481 aaggacgggg ataatatgaa agtcaaggta gctgtaaaat tccttgataa tcagacaaag
      541 gcgacgcagg tattgcagta tgagcttatt ctacataagg atagtaactg gaaaattatt
      601 atgtaagaca ttttgttggc aggatctatt ttcggatata gtctgccttt tctgtagcaa
      661 atgtactgca ctgacaagct atttcattgt attttaagca attaaacctt tcttgacaat
      721 tttatcttaa atcatatatt tatggatatt gactttctgc ttgcaataag ctataatatg
      781 actataaaat caaatcagtc atataggagg tatgtggtat gtataaagaa gttggtattt
      841 cttcaaataa tgttccgata acactttcta tctggtatac agaaagaagt agtcctacca
      901 ttgttttttt gccgggtact atgtcacacc ctctcatgta tgagaatttt ctatgtggtt
      961 tgagtgaaag aggatttaat ataataggca ttcactatct ttcacacgga aaaagcccta
     1021 aaatcaaaaa aaattataca atggaagatt tgctgcataa tgtatatgac gcaacaactt
     1081 acggaataga aaatttcggt gaaaacatag ctgtcatggg ttccagtcaa ggcgggattt
     1141 tagctgcaat ggcggcaggt agggacacaa ggttaaaagc cgtttttcca cataatatca
     1201 tgctgaccac attaaaagaa actatgagct taacaaaata tcccacatgg tcgcaccgtt
     1261 ttatggggct gatacagtgg ggctttcgtg taggtggaaa attgctgcct aactacaagg
     1321 ttccaggaga cgcttattta gactatgaca gagtatttta tagtgaacag gcaaaacaag
     1381 attgcttaaa tgaccctatg cttttaccct attatccttt aagatttgtc accagcttat
     1441 ttaatgctga cttatcgtgc ctggagaatg gggctataca atgtcctgtt attttaatta
     1501 ctgccaaagg cgacccgctt tttagctttg attataccaa caaagtattt gatttaattc
     1561 atgtaccgca aaaggaatta cttgctttag agcttaatcg tcacatgatt tttaatgagg
     1621 acacggacac tacaataaac gcattggaag ctaccttgca taaatatctt ggatagcata
     1681 ttgattatat aatcagtaaa tgttatactt ttcatgacga tataaatatt ttagtcataa
     1741 ggaggttatg agtttgccac gatttacgga acaagaaaaa gaaatcataa acagtaaact
     1801 tttgatagag ggagaaaagc tgtttgcatt acacggctta aaaaaagtaa cagttgatga
     1861 tttagtagct gctgtaaaca tatcaaaagg ttctttctat gctttttatc ccagtaaaga
     1921 acatctatat gttgaaatca attttcgctt acaaaaagag ttatttacaa gtatagaaac
     1981 aaccattaaa aagaaaaagt ataagaacca tagagattta gcaaaggacg ttatcatgct
     2041 aagtttaacg ggggtgatta cttcgcctat tctttcacag attgatttat ctttaatgga
     2101 ctatctgcaa agaaaaatat cgtcagatat ttttgaaaat catatgtaca gtgatattcg
     2161 tatattggag atattggaag atttaggggt tgtatttttg gttccgcata cggttattat
     2221 aaaatccctg tattctgtct tatcctgttt ggaacaattc aaagaggacg aagaactgaa
     2281 tatgatacaa aatcttttag tgaatggtat tattcagcaa gtagtagcag agtaacaatt
     2341 ttgtttggtt atttgacagg caggtagcca aacaaatttt ttacagttta tatgactata
     2401 tatctataaa cgtcataaag ttaaaggagg aatgaataat gattgatgta aaaaaactgt
     2461 attttagcta tactgacaaa ccgtttgtgg agaatgtaag tttccatgtt gggagaggag
     2521 aaatttttgg ttttttagga ccctctggtg ctggaaaatc aactattcaa aaagttttaa
     2581 ctgggcttaa tacaagatat aaaggcagtg taaaagttgc aggtacagaa ataagggagc
     2641 atacaaatca gttttatgaa aatattgggg tagactttga attttcaacc tgctacgaga
     2701 aattcacagc acgacaaaat ctcgcctatt ttgcttctct atatgaaaag caacctcggt
     2761 ctattgatga attattgcat atggtaggct tggaaaatga cggggacaaa aaagttgctg
     2821 acttttccaa aggtatgcgt tctcgcttga atttcattaa agcgttagtc catgaccctg
     2881 atatattatt ccttgatgaa cctacaagcg gtcttgaccc tacaaatagt cgtttaatga
     2941 aagatattat tcttgcggaa aagaaacgcg gaaaaacgat tatcattact actcacaata
     3001 tgtttgacgc aacagagcta tgtgaccaag tagcctttat cgttgccgga aaagtcagtg
     3061 cattggatag cccgcataat ttgattatgt caaggggagc tgccaaaata caatatacct
     3121 actatgataa aggcgagaaa accggagaat gtctgcttga tagaaccaca gaagataagt
     3181 tgttaaaaag tttaatttcc gaaaaccgtt tgctttctat tcatagtagt gagcctacat
     3241 taaatgatat ttttgttgat attacaggga ggacacttca atgagattgg gacgtttaat
     3301 ttgtggtgat atacatttcc aatggaaata tggtttctat tttatctact ttatattaac
     3361 agttttatat gtgtgcggga ttgccgcttt gccggaacat tggaaaacgg atattgcttc
     3421 tattatgatc tactctgacc cggcagcaat gggattattt tttatgggag cgattgtcct
     3481 cttagaaaaa agtcaaaagg tgttaaatgc tatggtggta tcccctgtaa aaatatcgga
     3541 atatatactt tcaaaaacag ttgcgttgat tgctatttct acggtaatcg cattgatatt
     3601 aggggtagtt tccggcagca atcatttact gggtatcgca gttggtacgg ctttgacctc
     3661 agcaatcttt actatgcttg gaattattgc agctacaaaa atttcaaact tgaaccaatt
     3721 tctcattgtg attatgccga tagaaattat ttgctttgta ccacctattg ttggtctgtt
     3781 tgtgaagtta ccatatctgt tccgcttttt tccattcaca gcttgcatga accttataac
     3841 tggaaaaagt gttttactat cgtttgatat ggtacttgta attgctacgt tgattatcct
     3901 ctacatagtt gctcgacata cagttgagca tatgtggaaa agtttgggag gtgtaaagct
     3961 atgaaaaaga tattatccag caccatacag atatttaagc aaatcaaaag tgaccctatg
     4021 atgtttgcgg cttgcttctc cccttttatt atggggactt taattaagtt tggtattcca
     4081 tttcttgaaa gaataacgaa gttttcttta caagcgtatt atccgatttt tgatttatta
     4141 ctttctatca tggctcctgt attgctctgc tttgcatttg caatgattac attggaggaa
     4201 atcgacgata aagtgtcacg gtacttttcg attacccctc ttggtaagtc ggggtatctc
     4261 tttacaaggt tgggagtacc ctcaattatt tcggcggtca tcgcttttat tgtactgtta
     4321 cttttttcat tagaaaagtt gtccattgga atgacaatat gtttggcgct tctcggctca
     4381 gtacaggcaa ttattgtttc acttatgatt atcacattat caagcaataa attagagggt
     4441 atggcagtta caaaactagc tgcacttaca ttgctcggaa taccagcccc cttttttatt
     4501 gatagttact accagttcgc ggttggcttc cttccatcat tttgggtagc gaaagccatg
     4561 cagaatgaag cggttcttta tttctccata ggattggtgg tagctttagt ctggtactac
     4621 ttccttacaa aacgtctgtt tcggaagctg gcaggataag gaggcatatt atggaaaaag
     4681 gtagacttct gcaaaacaaa aatatgaata aggctattca ggtttccaaa attacactaa
     4741 tcatcaatct gagtttatcg ctacttaaat ttgctgccgg atatatagga aagtcaagtg
     4801 ccatgctgtc ggacgctgta cattcggctt ctgatgtagt aagcacaatc gttgtgatgg
     4861 ttggaattaa aatatcagaa aaacagcctg atagagaaca tccttatggg catgaacgta
     4921 tggaatgtgt ggcttccatt attctctctg ttgcattggc tataacgggt gctggaatag
     4981 gatattcagg tatcaagaaa atcttttcag gacagtacaa tacgctttct gtccctagtg
     5041 gaatcgcact tacggcagca gtcttatcta ttgttataaa agagtggatg tactggttta
     5101 caagaagtgc tgcaaagcat acaaattctg acgcacttat ggctgacgca tggcaccacc
     5161 gctctgatgc actctcgtcg gttggttcct taattggcat tttaggagcg cgtttaggct
     5221 atgctattct cgatcctatt gcaagcgttg tgatttgcgg ttgtatctta aaagctgctt
     5281 tgaacatttt caaagaaagc attaacaaaa tggtagacca tagttgtgac aatgctactg
     5341 aaacaaggat tagggaagtt gtgcttcagc agcaaggggt tgatggtatt gatgaattaa
     5401 aaacccgcat gtttggagcg aaaatgtatg tagatattga aatattagct gatggtaatt
     5461 tagctcttta tgacgctcat agaattgctg agggagtcca tcaaacgatt gaaaacaact
     5521 ttccacaatg taagcattgt atggtacatg ttaatacgaa tgaacttctc tattctgcat
     5581 aggacaggga agatgattta aggctattga aagggaaagg agggaagtag gttatggaac
     5641 aatactggtg gattatcatt gttgtggttt tgattgtcgt tggcttactt aaaattgtaa
     5701 ttatgaagaa gtataaccaa aggagaaatt ctaacaatcg ttcaaaagat agcgaggacg
     5761 acaagatgta ttaaggttta ttgaaatctg ttatgttgta gaatgatgtt tttagatact
     5821 agaaagtgct ttaagggggg ctaacgttaa ggaaagcttt gagtacaatt ctttacaatc
     5881 aacagtcttt tgcatagaga aatgtatgta ggaaatatgg tgcaaggcac aaaacaccag
     5941 caattgagaa atcaacctat gaaccaaagg cacactacat tttatactac ttgacaagta
     6001 aacgaacatt tactttaatg gtttaaagaa gtaaacgttc gtttactaaa gaaggaggtt
     6061 gtaaatgtct gacacaaaag aaaatatatt aaaaattgca ctacacttat ttgcagtgaa
     6121 tggttatgag gctgtttctg ttagtatgat tgcggaagcc ttgggaatga caaaaggagc
     6181 attatataaa cattataaaa ataagcaaga tatttttgac tgtatcgtag aacgtatgta
     6241 tcaaattgat aataagcgag ctaaggatta tcaagtacca gaaagcacaa tagaggtaac
     6301 cccaaatgca tacagaaata caactattgg aagtctaata acatttactc ttgcacaata
     6361 cgatttttgg gtgactgatg aatttgcgtc taattttcgc aaaatgttaa tactagagca
     6421 atatagaaat ttaaaaatga atatgcttta tcaaaaatgt cttgtaaatg gtcctatcag
     6481 ttatatagaa gatatattta atgaaatgat tgaaaaaggt gttcttcaag agtgtgatac
     6541 aaaacagctt gctattgaat attatgcgcc attatttcta ttgataagtg tagcagacgc
     6601 agttgaaaat tgttgggatt taaaaactca attaactaca cacgttaaac aatttgtcaa
     6661 gaaatatatt ataaatgaga aagtaaaata atcctttatt ctgtcctaaa tataaaccta
     6721 actttttaat ttcagcagag aaactcaaaa tgaatattgt tataagtcac aaatattaac
     6781 tattaaaata ttacaataac ggagatgaga aaatggaata tattcgcagt aaaaaatatt
     6841 ttacacccga cttacagaca aaaattatgg ggccgaatcc tataaaatta caagaggaac
     6901 tattacaaaa tcataaaatc ccgtctggta gctatgtctg tgaccttgga agtggacaag
     6961 gtttaacttc tgtatttctt gcaaaagaat atggatttgt tgtttttgca gttgacttat
     7021 ggagtgagcc aaaagaaaat gaagaatttt tcaaaaaaat gggattatct tctgcacaaa
     7081 ttaaatctgt aaaagctgac gccaatgaac ttaaatttga gaaagagttt tttgatgctg
     7141 tagtaagtac agattcatat aattattttg gacgtgaccc tatatattta gatgaaaaat
     7201 tgcttccatt tgttaagaaa ggtgggtttg tttatatcac agttcctggc atgaagaaag
     7261 attgccatga gaaacttcca gaagagttat tgttatcatg gacaccagaa gaacttgatt
     7321 atatacatga tataacatat tggtctaaca taattagtca ttgtaaaaag gcagatttaa
     7381 tttcaatata tgaaatggaa agtaattcgg aagtttggtc ggattggata aagcaaaata
     7441 atgaatatgc aataaatgac cgtaaggcaa tagaagctgg cggtggagat tatctgaatt
     7501 ttattgctat aattctacaa aaaaaataaa ttctttagaa ccaaacacta taatttgcgt
     7561 tctaatatgt ttaccgattg acatcaataa ttttatcttt gagggtaatg atttgaaaat
     7621 agaagaatac aagaagatac gaaattgata aattagaatt tgatgggatg gtgatattat
     7681 gaaaatatta tggatcatta ttattatggc agataatctg ctgccgttac tgcttgctcg
     7741 tttctatcca aactataagc ataaagaaat ggcactatct gtccttggct ctcgtcaaag
     7801 cccggtgaaa tggatatata atgtgtggtg cattatatca ggtgtggtat tttgtatcgc
     7861 tccatatgcg ttgtatcaag agaacgatag cggatgggca atcgctatat ggatgcttct
     7921 tgctatatac ggcatcgggt gtgaaatcat ttccggagtg tgcccgttaa atgaaaatag
     7981 acaagacgaa gatataatta caaaaattca cggcggcaca tcggcaatcg gatttatggc
     8041 cttgcttgtc gttcctttgc tctttgcaat tctgcaattc caaacaacaa aagtagtgtt
     8101 aggcgcaatt tcactggtgt gctttacaat agcattcata ttcttctgct tttttattat
     8161 gggtgagaag gaaaagtttg caaatactgt tttgagatat ggcggtcttt ggcaaaggct
     8221 tgttttaata agttgttata taccgttatt tgtttggtct gtttctaatc aataaaattt
     8281 agtttatagg aataacaaat tccagtttgt aaaagtcaat aaatccaaag ataaatttgt
     8341 atttgaaagg agttttgaag aatgaaaaaa tatgagtatg tagaaattaa ctataatgcg
     8401 aaaggagttg tattcctttg tactgataaa cacaaagaaa ttattgacag ttacgcaaaa
     8461 acggggtatc actacgcagg gtttgtaccc acagaaattg acgcaaaagg ctgtatgcga
     8521 aagatagatt taatttttga aaaagaagat taacttattt tagggagttg gttctaatga
     8581 taggacttaa aagacgagat atgaattata ttatgaaagt tggtgctaaa gctcaaggtg
     8641 tttctgtatc tgaatttagg gctatgctac aagataccat agacaaaact atggaaagta
     8701 cagacccaga cgtgcaggca aattttaaac gatactttgg aaataagcgc ccaacaccag
     8761 aggaatatat ttatacgatt accaaaaaga caaaggttaa ggtttaaatg ttgtaaaggg
     8821 ggcttgccgt atgaaacgct ttatgtacaa ttcagtatat tcaagcccaa tttaacagca
     8881 tactatttac aattaaatac gctataccag tagctcgtac aaaatttata ttttgtgcgg
     8941 gcttttttaa tacccgaaaa gtttttttaa ttattttcat ttttttggaa gcaaatcaca
     9001 ccttgctgta cagatatggt gcggaaagag ggataaacac ttttcaattc atagctggaa
     9061 aggaggtgag attatgaaac cgtctgactt ccaaaaaaca attcagtgtc agtttgaatg
     9121 taagctgaaa aaagttgtca aaggtattat ctggaactac cgtaaagaat taaaacgaag
     9181 aaaaaacaag gaaatatctt ttagcgaatt accagacatt acggtagaaa gtctggcaac
     9241 atgggacgac tacgaaacaa actacacaat ttttaatgtg tgtggcatag atattaaagt
     9301 ccttgatgat gaacttgcag aagctctcaa acagctaccc gaaaaaaaac gtaacaatgt
     9361 attgatgtat tacttcttgg aaatgagtga tacagagatt gcagatatta tgaattatac
     9421 cagaggggct gtgttcaaac acagacaccg tgcactagaa actatgaaag agatactaaa
     9481 ggagaagaaa tagaaatgaa gaaaaagtac aagcacccgt cttattattt gatttcatca
     9541 gctatggatg gggatgaaac tgcgattgag aaattactgg cattctatga cccgtacata
     9601 tcaaagtgtt gtttacgtcc tttatatgat gaatacggca atgtttatat tgttgttgat
     9661 atggaattaa agggacgtat tcgagaagcc ttaatcaaaa tgattttaga ctttgagatt
     9721 gatatagata tagaaaccga agaagaataa gcaaatacca gcagggcgtg attggttaaa
     9781 ttcccttctt tcctgccctg ctcctgcttt tatacatgga agcctacggc tttcgccgat
     9841 tgttctttga caactgaata aagcagacag atacgtttgt gatacagcga gccacgggga
     9901 ctgtacgcca tgacctttcc gacaagaaag cgagcgaccc acgcagttga tccgagagcg
     9961 acttgtggaa tggtcgtttt gccatgacct gctatcacag aataatgata cgtccgcatg
    10021 gtgcggttct gcccacaaga atgggaatag ttgagatact aacggagctt tccaaagccg
    10081 tctgtctgct tatgataaga cacacagaaa ggagttgttg caacatgaac aatactgatg
    10141 tgccgatttg ggaaaaatac acccttagta tagaagaagc gtcccgatat tttcgtatcg
    10201 gggaaaagaa gttgcgtaaa cttgccgagg aaaatctgga ctctggttgg gttatcgtta
    10261 atggaaaccg tattcagata aagcgaaaac agtttgaaaa aattattgat acactggacg
    10321 aaatctgatg tcttagagcc gtagatatgg tataatatta gtagcgtatc acggctcatt
    10381 ccatgacgga aaggagcttt acactatgtc aaaaatgaaa agacaagaca gtaaaggacg
    10441 caatttgcgt cttggagaga gccagagaaa agacggaaga tacgtttata aatataccga
    10501 tatattcggg aagccacagt ttgtatatgc ttggaaactt gtaccgacag acaaaacacc
    10561 tactggaaaa cgtgatgata aatcattgag ggaaaaagaa aagcagataa aaaaagacct
    10621 tgacgacggt atcgacacta tcggcggtaa aatgacagtc tgccagcttt atgcaaagaa
    10681 gaacaaccag aaaaagaaca tcaagagaaa cacggaaata ggacgacagt atcttatgaa
    10741 cgcactagaa aatgacccat taggtatgag ggcgattgat acagtcaaac agtccgacgc
    10801 taaagaatgg gctatcagaa tgaatgacaa aggttattcc tataagacaa tagataacta
    10861 taagcgttcc ttaaaggctt cattctatat ggcgatacaa gacgactgta tcagaaaaaa
    10921 tccttttcat ttcaagctaa gtgatgttct ggaagatgat acagagccta aagttatctt
    10981 gacaccagag caggaagaaa aacttctttc ctttatggaa acggataatg tttacagcaa
    11041 gtatcgtgat gaagttattc ttctgctgga aacgggactt cgtatctctg aattatgcgg
    11101 actgacaaca cacattgata tgcttaatcg tgtgattaac atagaccacc agctattacg
    11161 tgatacagag gtgggctact atatttccac acctaagaca aaaaacggca agcgagagct
    11221 tccattgaca gaaagagcat atcaggcact tgaacgaatt ttaaagaacc gaggaaaagc
    11281 tcaaccgctt gttgtagacg gttatagcaa tttcttgttc ttgaaccgtg agggcttgcc
    11341 aaaggtagca ggaaattatg aaagtatggt gcgtgggctg attaagaagt acaacaagac
    11401 ccatgaggac aagttgccga acatcacgcc ccactcattc agacatacct actgtacgaa
    11461 catggcaaat aagggaatga accctaacac cctgcaatac atcatgggac acgcaaacat
    11521 aaccatgaca ttagggtatt atgctcacgg tactttccag tctgcaaagg cagagttgga
    11581 aaggctggct tcttaatatc gaaaatgtat cttactactt atttactact tttggttgca
    11641 ttttcatgct ggtaaatgcc agcttatgcg aggtatcttc caaaaagaaa atgcccatac
    11701 agcccgaaaa tacgggatat atcggcattt accaacttat gaaaagataa tcagaaatta
    11761 gtaagttttt atatcaaaaa aatgattact gtaaagaaat cattttttat tataatcact
    11821 gatacatcaa atattctaaa taaaattttt atttatctgg aattgtatat tttttctata
    11881 atattaacta cagcgtacat tgcccaagca cagatagcca atacaaatac tcccatcatt
    11941 actaaatcta atttaaatac ttgagaacca tatactatca agtagcctat tccatatctt
    12001 gaaactaaaa attctccaac tattacacct acccatgcca taccaatatt aattttagtt
    12061 aaatttatta aatttcctgt gtttgcaggt aatatgagtt taaataatat ttgagattta
    12121 gtagctccaa aactttttaa catcttaatt ttttcttcat ctatattcat aaaataatta
    12181 taagctgaaa gtatagtaac aacaacagaa atcgttacag ctgttacgac gattccttct
    12241 attccagcac ctacccaaac tataattata ggagctaaag cagtcttagg aagagcattt
    12301 aaaactacca aaaatgggtc taatattttt gataattttt ctgaccacca aagagcaatt
    12361 gctaccaaaa tacctaatac agtacctata acaagaccaa gaacagtttc atatacagat
    12421 attccaatat gtttaaataa ttgaccacta aatgcatatt gaataaataa attgtaaata
    12481 tcacttggtt tgctaaatag aaatgtattt ataacattta agtcagctaa aagttcccag
    12541 attacaataa aaccaattaa aattaatagc tggtagaata atacatttct tttttctctt
    12601 tttacacctt taacatagtt tatatatcct tcagatcttt tattaagcct catgtgtatc
    12661 tagctccttc cataatatgt cgaaatactt ttgaaaatca ggaactttac gtgttttgag
    12721 aggcgttttt gttccatcca tgttaagttc aatatcgtaa atatttttta tggtagctgg
    12781 tcttttagat agtattgcaa ccttatctga tatgcttatt gcttctgcta tagctaaaaa
    12841 agtgcaacta attgcttaaa gaaacagaat tatcaacttc agcacccata taaacaatat
    12901 taagatttcc taaactgcta tcccaaacga tactattaat aatagtagat aataacaatc
    12961 tcttatcatc taaactggca ttatcaactt catcattaaa tctcttcaaa ttactaacaa
    13021 taatatcaat attaatactg tcactactaa tattgatgtt actagattcc aaaatattaa
    13081 tcttatcctt taaaatacta atctcattat ttaggctctc aatctgggaa actatataat
    13141 cagaagcagt actaccagta ttttttgcaa gctctataac taaattttta ataagtcttt
    13201 ccttatctaa aatttgattt ttaagtaaat tagtctcaga tttaaaaata ttaaaaacat
    13261 tatcagtctt aatgttatta ctattgaatg cacttataat agactcttct ttacaattct
    13321 taattttatc tataactaaa ctttcaacta atggaccatt aagattttta cagttgcaac
    13381 tatcagaccc aagtgttttt ttagtaccac aaatataata atactttaaa tccttgtcag
    13441 tttttttgcc tttataggta attctcatat tagaaccaca tttaccacat tttataagac
    13501 cagataaaag agcagttttt ccagttgata tccttggagt tttatcacga tttttatcta
    13561 attgattttg tacttcaagc catatttcag gttctatgac tccatggtgt tttgcaattg
    13621 ctgctattga gttagatgta tttttagcat aggtaaggat tccacatttt ctatctggag
    13681 tacctacaac atccatacca ctatttttaa ggtaatctaa aatttcatca ttagctttta
    13741 catatgctgg atttctaagt attctagata aagaactagg gtcaaatttg ccaccacgag
    13801 gaccccttat tccattatta tagacatatt tatataactt agataaggac ctaagttcta
    13861 aatatttatt gtatattagc tttacaatct ccatttgttc atcatttact tgaagcttat
    13921 acatacttct ttcttttaga ttttcatcta agaatgtgat tttttcactg ttgaatccat
    13981 gaggtgtatt gcctccaagc catctaccac tacgggctaa ttcatacata ttgtctttta
    14041 tacgttctgc tattgtttct ctttctaatt gagcaaatac acttgatata aacatcatag
    14101 ctcttcccat tggagttgaa gtatcaaatt gttctttgat agatataaat gatatgctta
    14161 gttcatttaa tttttctatt aaagtagaga agtcagatac atttctcgat atcctatcta
    14221 atctataaca aactaggtaa ctaaatttct tatctttggc atcctgcatc attttcttaa
    14281 attgtggtct atctatattg ctaccactaa atccctcatc ttcatatatc aaaaaatctt
    14341 ttacaccaat agactttgca taatcaatac ataattgtat ttgattttct atagaatcac
    14401 cttttccagt aaatcttgat tttcttgaat aaattgcagc tttcaaaatt aaaaccccct
    14461 ataagttagg atatatattt gaaactattt atctacaacc ttttaagtaa acttattaga
    14521 atgaatttat taattaggta aatttatata attctacttt ttataatata tgactattta
    14581 ttttatttct ttactattca agttctaaat caataataaa ttttgatatt taattgtatc
    14641 aggacaaatc tctaatttca tagattatta atataaaatc ataggttagt gtgaaaaagt
    14701 tgaaatttaa atattttaaa agataatttt tttgaactaa taggtataac aaaaatttat
    14761 agggggattg catatggaaa tcaatgttat agaaattttc cctaaagata aagctaaact
    14821 taataaaata gaaatggata aagctagttg gtttgtaaat ataataagta aaaaatatcc
    14881 taaagaagct ttaaatgaag catttagtac tttagaaaaa gaattaaata taagtaaagc
    14941 taatacataa gctttgatta ttgattttat atactattgg ttttatacgc tatgacttga
    15001 cattatatta atcaaatagt atatttttaa atatagcaca atgaagtgtt aaaagagcaa
    15061 tggagctcca tagataaagt ctatggagct tttttgcgta tttaacttaa ggaggatagg
    15121 actttgaata actttgagat taaaactaga ataaaatttg gagaaggctc actaaaagct
    15181 ttgcaagaat taaaaaacaa aaatgtacta ataataacag acccttttat ggtgaaatca
    15241 aaaacaatag ataaaataac atctaattta ataaactcaa catatcaagt ttttgaagaa
    15301 atagtaccag acccaccaat agaagtagta gtagcaggaa tagaagcatt taaatctata
    15361 aatccagacg taataatagc attaggtggt ggttctgcaa tagatgcagc aaaatctata
    15421 atggactttt ctaaaaaaat attaaaaatt aacgaagtag agtttatagc aataccaaca
    15481 acaagtggaa caggttcaga agtaacatca tttgctgtaa taacagatag tcaaaaaaaa
    15541 tgtaagtatc cattagtgag tgatgattta cttccagata tggcaatact agacccagaa
    15601 cttgtaaaaa ctgtaccaaa ttttataaca gcagatacag ggatggatgt attgacacat
    15661 gctatagaag cttacgtatc tacaaattct aatgactttt cagatgcact agctgaaaaa
    15721 gctataaagt tagtatttaa atacttaata aaagcatata aagatggaaa tgacatagaa
    15781 gctagagaaa aaatgcacaa tgcatcttgt ttagcaggta tggcatttaa ccaagcatca
    15841 cttggcataa atcattctat tgcacatgta ttaggtggaa aattccatat accacatgga
    15901 agaacaaact ctatattact tcctcatgta atagaatata attcatcaat agtaggatat
    15961 actaacacaa cttactctgc tacagctaaa aaatatgcac aaatagcaga attagtagga
    16021 cttgggagtg ctaatataag aatatctgtt agaaatttag taaatgaaat aaagaaacta
    16081 caaaaagaaa tgaatatgcc aactaaattg acacaatgca aattaagttt agaagatata
    16141 actaatgaag aaagagaaat agcaaaactt gctataaaag atacttgtac attaactaat
    16201 cctaaggtac caactgaaga tgatataata aacatattaa agaacattaa ataactacat
    16261 tttattacat gtaaatataa tattatacaa atagggaaaa tgtttttaaa aatataaatt
    16321 atatattgac aaatataaaa cataaaacta taatgaaagt atcacaaagg cgtgatactt
    16381 tagtagaaag caatggagct tcttaggaat attaatatta ttcttatgga gctcctttta
    16441 ttttaatcaa atagcttttt aataaactat attcaaagaa agcagggaat aaatttgact
    16501 gaagaatcta agcaaagagt tattcaagaa tatgttcctg gaaaacaggt tacattagca
    16561 catatcatag ctaaccctaa tgaagatata tataagaagt taggattagt acttgataaa
    16621 aaagatgcaa taggaatact tacaataaca ccaagtgaag cgtcaataat agcagcagat
    16681 gttgctacaa aggcttcaaa tgtatcttta ggttttatag atagatttag tggttcagta
    16741 gtaatatcag gtgatgtaag ttctgtagaa tcagcgttaa atgatgtttt agaagtctta
    16801 ggaaatatgt taaacttctc atctacaaaa atcacaagga cataataata caatgaagaa
    16861 tataatattt atgggaaaga caggtagtgg taaaactacc ttatgtcaaa aactagacca
    16921 gttagaatta aagtataaaa aaactcaatc agtggaactt tataataatg caatagacac
    16981 tcctggtgag tatatggaaa atagaaacct atataatgcc ttaataacaa catcagcaga
    17041 tgctaaaatt atagcaatag tgtatgatgc cacacaagaa gaaaattata tagcaccagg
    17101 ttttgccagt atgttttgca aagacgtaat aggaattata actaaaataa ataaagctac
    17161 tgaaaacgaa gtcaatatag cttatgaaag actatcacta gcaggagcaa gtcaaatctt
    17221 taaaatagac acagttgata atattggact tgaaaaatta tttaaatatc tagaaaaata
    17281 taaaaatata gatgaagttt aaggggaaat atttttttat tcaaaaatat tattaaagta
    17341 tgataaaata aaaaaatgta taaactattt taagaagatt taatctttaa gcgaggtagg
    17401 aacatgggga aaaaaatact attggttgat gatgaaccac tgttaaggat ggatacaaaa
    17461 gatatattag aaagtcatgg ctatgaaata ataggcgaag caagtgatgg atttgaagct
    17521 gttgaaatgt gtaaaaaaca caaacctgat ttagttataa tggatataga gatgccaata
    17581 cttgatggta taaaggcagg caaaataata tgtaaagaaa atatagctgg tggagtactt
    17641 ttattaactt catttgaaga taaagaatac gtagaaaagg ctaaaagtat aggtgcttat
    17701 ggatatttag taaaaacagc aagtgaaaaa tcacttatcc caaccataga aatgtgttta
    17761 aataaggtaa atgaatttga aaaaatgaaa aaagatttag aaaaagtaaa ttctaaattg
    17821 aatgaaagaa aattagtaga aagagcaaaa ggtatactta taagagagtt taagatttct
    17881 gaggatgaag catacactag aataagaaaa ttaagtatgg ataaaagaac tacaatgatg
    17941 gaaattgcca aaatgattat aataggatac gatgactaat acgataaaaa aaatatcaac
    18001 tgcacaaaca tacttaaata ataacgaaat tgaaaaaatt atagaagtat caaaatcctt
    18061 agatttaatg gcttcttttt atgaagctga tgtttttata gatgtactaa gtaaatttga
    18121 tgatgaagca attgttgtgg cacatggtag acctaaagaa aagtcaatgt actgtgaaaa
    18181 tgttataggt aaaaaggctt taattgaaaa tgaaccagga gttataaagt gcttaaaaac
    18241 aggagaaaga gtaagagata ttaaagcttt aacacaggaa tataaactgg tcaaacaaaa
    18301 tattcagcca ataacactag atgacaaagt tttagcagta ctaatagttg aaaaagatat
    18361 aagtagtgaa gttaagaatg agtttaatgc tagtgaagac gaagaaaata aaaatttaca
    18421 tcagtttata aaacttattg gaaataatga ctttttagtt gataatttaa attcagccat
    18481 attaatattt gataaaaatg gaaaattaaa aattaaaaat cgtattgcaa ctgagatata
    18541 caaaaatctt gggtttggat atgatataca agatatgcat tacaatgatt tagatataga
    18601 aaccaacacc tttgaaaata tattaatgga tgaaaattat gatgagacaa atgaagtttc
    18661 aataggtaat atgtttttta aaataaagac catatacatc aaagatgatg aatttaaggt
    18721 ttgcaaaata gtacaagata taagtgattt aaagagaaaa gaagaagaat tagttttgaa
    18781 aacagtggct ataagagaag ctcatcatag agtaaaaaat aatcttcata cagtaatatc
    18841 tataataaaa aaacaaagta gattatctca aaatgatgaa gtaaagcagt gtttagataa
    18901 cataataaat agggtatttg caatactttc gactcattat ttactatcta aagaagttga
    18961 taataatata tctatacgag aaggtatcaa tttattagtg tcaaatattc aaggtggcta
    19021 tttatttagt aaggatatag atatacgtat aactggtgaa gattttaaaa ttagtggaga
    19081 aaaagcaaca gcaatacttt tggttataaa tgaaatacta caaaattgtt atgaccatgc
    19141 attttcggat agagaacatg gtactataca aatattaata aataaagata acgactatag
    19201 aaatattgct attatagatg atggaattgg atttgatagt aataatataa ataaaaatag
    19261 cttagggaca tatataatag atagttatat aacacagatg ttaaaaggaa atatagagag
    19321 aaaaagtagt gagaatggta cagaagtatt aattactata cctacacaaa ttaatattta
    19381 aataataact catctacaaa gtagtagttt taattgagca aagaggctta aggttattaa
    19441 tagattaagt taattgaatg tatttataaa aattataagt atatattcaa tttaacaggt
    19501 atttattata actttaagcc tctttgcttg tttaagacat acttaaattt taggaggtga
    19561 gaaaatgaga gaagagttat taagtgttgg tattgacatt ggaacaagta ctactcagtt
    19621 aattttttct aaattaatag tagaaaatac tgaatcaaat ttttcggtac ctagaataag
    19681 tatagtagac aaagaaatta tttataaaag caaagtttat ttcacccctc ttatttcaaa
    19741 taatgaaata aatggtcaac aaataaaaga tatagttgaa aatgaatata aaaaagcaaa
    19801 tatatctaaa gaagatatac atacaggagc agtaataata actggtgaaa cagctagaaa
    19861 agaaaatgca aatgatgtac ttcatacttt gagtggattt gctggggact tcgttgttgc
    19921 aacagcagga cctgatttag aaagtataat atcaggaaaa ggagctggag ctcatattta
    19981 ttcaaaagaa cattcaacta caattgtaaa tatagacata ggtgggggaa ctagtaacat
    20041 agcctcattt aaaaggggag aagtaaccga tactgggtgt ttggatatag gtggaaggct
    20101 tgtaaagata gataaaataa gtaaaaaaat aacttatata tcacctaaaa tagataaaat
    20161 cataaaagat aataatttaa atcttcaagt aggtacaatg gctactgtag aaaatacaaa
    20221 gccaatagtg gatataatgg ttgacttatt aatggaaagc ataggactaa agaaacagac
    20281 acctttgttt gattttataa taaccaataa atcaataagt atagatgaaa atattaaaaa
    20341 tgtttcattc tctggtggag tagctgatta tatttactat gatggagaaa taaaagatta
    20401 ttttgaatat ggtgacttgg gaattttact tgggcaagca ataaaaaatt gtgaatacct
    20461 taaaaaagta aatgtagtta aatctatgga aacaataaga gccacagtgg ttggagcagg
    20521 ttctcatact actgaagtaa gtggaagtac aataacttac actagcgaaa agtttccact
    20581 taagaacttg ccagttttaa aattatcaat tgatgaagaa acaggtactt caaaacaaat
    20641 cagtgaagct ataaaaaata aacttaattg gtttaaacta gaaaatgatt tacagaaagt
    20701 tgctatagcc atgataggaa agaaaaatcc aaccttttta gatatacaag agtatgctaa
    20761 agggtttgta gatggaatgc aacaaatcat agaaaatgat ttacctttta tagtaattgt
    20821 tgaaaatgat atggcaaaag tgttaggtca agcaatatat gcattattaa attatgaaaa
    20881 agaaattgtt tgtatagaca gtgtcaaggt tgaaaatgga gactatatcg acattggaaa
    20941 acctatagtt gaaggtaaag ttttacctgt ggtaataaaa acattagttt ttaattaata
    21001 gaaggaggta agtaaatgat tttaaataca aaattatttg gacgtactta tcagtttaaa
    21061 ggcttatatg atgtaatggc taaagctaat gaagaaaaat caggagataa attagcaggt
    21121 cttgcagcag aatcagctga agaaagaata gcagcaaaag ttgttttatc acacataaaa
    21181 ttggaggata taagaaataa tcctgcagtg gcatatgaag aagatgaagt aacaagaata
    21241 atacaagatg ctgtaaatga aaaagtttat gaagaaataa aaaattggac agtgtctgaa
    21301 cttagagaat ggctattgga ttcaaatact acaagtcttg atataagaag aatatcaaga
    21361 ggattaactt ctgaaatgat agcagcagtt gcaaaattaa tgtcaaatat ggacttagtt
    21421 tatgcagcta aaaagataaa ggttactgct cactgtaata caactatagg agaacaagga
    21481 acattatcag caagacttca acctaatcat ccaacagacg acccagatgg aatacttgca
    21541 tcacttcttg aaggtttaac ttttggtata ggagatgcag tacttggatt aaatccagtt
    21601 gatgactctg ttgagagtgt tactaaagta ttaaaaagat ttgaagaagt aaaacaaaaa
    21661 tatcaaatac caactcaaac atgtgtattg gcacacgtta ctacacaaat ggaagcaata
    21721 agaaatggtg ctcctgcaga tttaatattc caatctatag caggttctga aaaaggaaat
    21781 gaagcatttg gatttaatgg cgacactata gaagaagcta gacagttagc cttaaaacac
    21841 ggtacagcta caggacctaa cgttatgtat tttgaaactg gacaaggttc agaattatct
    21901 tcagaagcac atcatggaat agaccaagtt actatggaag ctagatgcta tggatttgct
    21961 aaaaagtatg aaccattctt agtaaataca gttgttgggt ttataggacc tgagtactta
    22021 tatgatgcta gagaagttgt aagagcagga cttgaagacc actttatggg taaactacat
    22081 ggcttaccaa tgggtgttga tgtttgttac actaaccata tgaaggctga ccaaaatgat
    22141 atggaaaact tagccatact attaacaaca gcaggatgta cgtattttat gggaatacct
    22201 catggagatg atgttatgct taactatcaa acaacaggat accatgaaac agcatcactt
    22261 agagaaatgt ttggactaac agcaataaaa gaatttgatg cttggatgga aaagatggga
    22321 ttctctaaag atggtaagtt aacagagttg gcgggagacg catctgtatt attaggatag
    22381 gaggtattta atatgaatga gaaggattta aaagcattgg tagaacaatt agttggacaa
    22441 atgcttggag aattagatac taatgttgtg tctgagactg ttaaaaaggc aactgaagtt
    22501 gtagtagaca acaatgcttg tatagatgat ataacagaag ttgatataag aaagcaatta
    22561 ctagtaaaaa atcctaagga tgcagaagct tatttagata tgaaagcaaa aacacctgca
    22621 agattaggta taggaagagc tggtactaga tataaaacag aaacagtttt aagatttaga
    22681 gcagaccatg ctgctgcaca agatgctgta ttctcttatg tagatgaaga atttataaaa
    22741 gaaaataata tgtttgcagt ggaaacttta tgtaaagata aagatgaata cttaacaaga
    22801 ccagatttgg gaagaaaatt ttctccagaa actataaata atataaaatc taaatttggt
    22861 acaaatcaaa aggttttaat attagttgga gatgggctta gctcagctgc aatagaggca
    22921 aatttaaaag actgtgtgcc agctataaaa cagggtctaa aaatgtatgg tatagattca
    22981 agtgaaattt tatttgttaa acattgtaga gttggtgcta tggaccactt aggtgaagaa
    23041 ttaggttgtg aagttatatg tatgttggtt ggagaaagac caggattagt tactgctgaa
    23101 tcaatgtcag catatatagc ttataagcca tatataggaa tggcagaagc aaaaagaaca
    23161 gttatatcaa acattcacaa aggtggaaca actgcagttg aagcaggtgc tcatatagct
    23221 gaacttataa aaactatgtt agataaaaaa gcttcaggaa tagaccttaa ataaagtcga
    23281 gggagtgtag aatatgaaaa atgatgtaat tcgtcctaat atactaggaa ttaaaataat
    23341 ttcaaatata agtccagaaa tggcacaaaa actagaatta aaatcacatc acaaaagctt
    23401 aggtttaata actgcagact gtgatgatgt tacgtataca gctcttgacg aagcaactaa
    23461 agcttcagaa gttgatgttg tatatgcaaa atcaatgtat gcaggtgctg caaatgcttc
    23521 aacaaaactt gctggtgaag tattaggaat aatagcaggt ccaagcccag cagaagtaag
    23581 aagtggtcta aatgcagtag ttgattttct agagtatgga gcaactttta taagtgcaaa
    23641 tgatgatgat tcaatagcat actatgctca ctgcgtatca agaacaggta catacctttc
    23701 agaggtagca ggaataagag aaggtgaagc attagcttat ttagtagcac caccacttga
    23761 agcaatgtat gcactagatg cagcaatgaa agctgcagat gttaagatgt gtgaattatt
    23821 tgcacctcca actgaaacaa actttggtgg agcattactt acaggttctc aatcggcttg
    23881 taaagcagca tgtgatgcat ttgcagaagc agtaaaatca gttgcagaca atccaacagg
    23941 attttaagga tagttagatg gatgctatag gcttaataga agtaatagga tatgttgctg
    24001 caatagaagc ttctgatgct tgtttaaaat ctgcaaatgt taatattgta agagttgata
    24061 aggttggggc aggtattgtt actttaacaa tatgtggaga tgtcggtgct gtaaaagctg
    24121 ctttagaagc gggtgaaata gcagcttcca gagttggtac attaagaaca tcacatatta
    24181 taccgagaat ccataatgaa gtaacaaatg ctttattcaa aactaaagaa cctaaagtct
    24241 gtgaagtttc agaagataaa gaaaaaaact ttgagattac attggaaata gctgataatg
    24301 tatcgaaaaa tttatcagaa aaagaaaatg taattttaga tgtcaatgaa aacagtggag
    24361 atactgaaaa tttatatagt gatatagata aaacaaacaa aaaaattgaa acagttgatg
    24421 atagtttaac agaagctaat gaggaaaaaa tagaaaacac taatgataaa gaagtattgg
    24481 aacaagttaa aatagaaaat tccgattttg atagagatga cctatcaaag aacagtgtaa
    24541 aaaaacttaa agctatggct aaaaaattag attcttcaat aacttataaa gacttaaatg
    24601 ttcttaaaaa agaagaatta ataaacttag ttaaaaagct tagcagaagg gatagatagt
    24661 tatgacactt ttagataaag atttagtatc tatacaagaa gtaagaactc ttgtaagaag
    24721 atctaaagtg gctcaagaga aactttctaa aatgagtcaa gagcaaatag ataaaatagt
    24781 aaaagctgtt gtagatgcag catttaataa tgctactaaa ttagctaaaa tggcaaatga
    24841 agaaactgga tttggaaaat gggaagataa agtaattaaa aatacttttg catctatggg
    24901 agtttatgaa actttaaaga acacaaaaac tattggaata attggtgaag ataaagaagc
    24961 caatacgata gatatagcta caccagttgg agttgttgca ggtcttatac cttcaacaaa
    25021 cccaacatca acagtaatat acaaaactat aatatctcta aaagcaggaa atactatagt
    25081 tttctctcca catccaaatg caaaaaactg catacttgaa acagttaata taataagtga
    25141 ggctgctata aaggctggag ctccagagga ttcaatatta tgtataacta atccaagtat
    25201 agttggaacc aatgaattga tgaaacataa agatacaaac ttaatactag caactggagg
    25261 agaagcaatg gttagagctg cttactcttc aggaacacca gctattggag ttggaccagg
    25321 aaatggacca gcatttatag ataaatcagc aaatgttaaa ttagcaatca aaagaataat
    25381 agattctaaa acttttgata atggtgttat atgtgcatca gaacaatcta tagtaataga
    25441 aaaatctatg gaagaaactg ttttaaaaga gcttaaaaat caaggtgcat atgttttaaa
    25501 tgaagaggaa agtgaaagat tagcaaaatt catattaaga gctaatggta ctatgaatcc
    25561 acaaatagtt ggtaaaagtg ttgaggtaat agcaaattta gcagacttaa caaatgtacc
    25621 taaaaataca aaagttctag ttgcaagaga aaataaagtt ggtcacaatg tagcctattc
    25681 aagagaaaaa cttacaccaa tactagcgtt ctatgttgaa gaaaatgtag aagctattat
    25741 gaacagatgc agagaaatat tgttaaatga aggtgcaggt catacattct caatgcatgt
    25801 aaatgatgaa gaattggtaa aaagatttgc ccttaatatg ccagtatcaa gaatacttgt
    25861 gaatactcct tcagcacttg gaggaatagg tgcatcaaca aaccttgtac cagcactaac
    25921 acttggttgt ggtgcaatag gtggaagttc aacttcaaac aatataggac cacttgattt
    25981 aataaatgtt aaaagagtag cttatggaat caaagaaata gaagaacttg gaagagataa
    26041 atctcaagtt gaaaaatcaa tgtttgactc aatggataaa gaagaattaa taaatcttct
    26101 tgttcaaaaa ctagtcaaag aactagcata actaaaataa acaatgaaac ttatggctat
    26161 cgctataaat ttctaaaaat ataaactata aaaaactata aaattaaatt tttggaggat
    26221 aagattatgg caagtgcaaa cgcattagga atgatagaaa ctaaaggatt agtaggagca
    26281 atagaagcag cagatgcaat ggttaaagct gcaaatgtac aattagtagg aaaagagcaa
    26341 gtaggtggag gattagttac tgtaatggta agaggagatg ttggagctgt taaagctgct
    26401 acagatgcag gagctacagc tgccgagaga gttggagaac taatatcagt tcatgttata
    26461 ccaagaccac actttgaagt agatgctata ttaccaaagg tatcagctga gtaatataag
    26521 aataattata aaatttaaat tttgattgaa aaagaggcta tctcaaaaat gagtcttact
    26581 aataaactca atgtattttg aggtagtctt ttttatacat ctaataggag atgtgataaa
    26641 atgggtgttt taacagaaaa cgaagtaaaa agaaaaattg gaaaaaacaa aaataatata
    26701 actgaatttt atatagaaaa aggtcaaata ataactcctt ctgcaaaatc ttatttagct
    26761 gagagaagta ttgagcttaa atatgttgat aaactttcag atgtaaacaa tgaacctaaa
    26821 gaagttgtaa aagaagtagt taaagagaaa aaatataaat atactactgt atttggcgcc
    26881 aaattggatg aaaaaccaga acatatgact caattaaatg gaaatctttt agtttttaaa
    26941 gaccataaaa gaataatatt gagaggaaaa atagattctc ttgaatcaga aatactaaag
    27001 gtacagatac tttgtcagaa actagatatg ccaaagcttg ttaaggaatt gcaagaagta
    27061 ttagactttg taagaaatat aatgagatgt gaagttttag atgaaaagct aggggacttt
    27121 aaacttttag gattaactcc tcaagaactt agagaacaat ctcactatcc taaaaaatac
    27181 tttggtatag gacatgagtt cccaagttat gaaatgggag aagtagtagt agctataaat
    27241 agtataagaa gtaatacacg agaggttgaa cttgcagcct atgaagcatt taagggtgag
    27301 tatggaaatg ttcacagaga agatttaata aaggcactta atagattatc gtcagtattt
    27361 tggataatga tatataaggt tagaactaat aagtataagt agttggagga cataatggag
    27421 aatctattaa atgaaatagt ggaagaagta ataactagag ttaaaaaaga agcatttata
    27481 gaagttgaag catcaggaag acacgttcac ttaagtagag aagatgtcga taaattattt
    27541 ggagaaggat atactcttac aaaactaaaa gacctatctc aaccaggtca atatgcatgt
    27601 aaggaaagag ttacaataac tggtcctaaa ggaagtataa aaaatgtagt tgtacttgga
    27661 ccatgtagaa atgaaactca ggtagaaata tctttaacag atggttcaac tcttggacta
    27721 aaagctccta taaaacaaag tggagactta gaaggtactt taagtattaa aatatcaaca
    27781 caatatggtg aagttgaact tgataagggg ctaatggttg ctaaaagaca tatacacatg
    27841 actcctaaag atgctgaaaa attcaatgta tgtgataaag agatagttga agctaaggtg
    27901 atgggtcaaa gacctttaac atttgatgat gtagtaataa gagtgagtga cagttttaaa
    27961 acttatatgc atatagacta tgatgaagcc aatgcatgtg gttattcaaa aggaactgtt
    28021 gctaaaataa taaagaaagc ataaatttaa cctactatat aaattacctc tagggaggat
    28081 ttaatatgaa ttatgataac cttgtagatt taataacgga agaaatctat aaaaaaataa
    28141 atagtaatga aataaaaata tctaataaac ctaaagctgt gattgtattt gaacaagata
    28201 acaataaatt taacttatta aaagatggat ttgaaatagt agaatttgat aaaagtataa
    28261 gggaatgtga aatagtgata gtttcaagac tttgtatgag ggggctatcg aatatagcac
    28321 ttggaaattc aacaagtgat gaagaaagat ttatactaaa aatgattatg aatggcaaaa
    28381 aagtatatgt cctagatgaa ggaattgaat acaaaaaata taaggatact gcaccaaaag
    28441 cactatataa gaaatttatg tcatttgaag atgaaatatg taaatttggt gtagaaatta
    28501 taaaagactt aaatgttata actaaaaata agttaaatat aaagaaagaa ctagaaagta
    28561 ctagaggttc taaaaaagaa gatacaaaag ttttagataa agatatttta aatttaacca
    28621 gtgtagaaag tgtaaaaata gataatgaat ttagtttgga tttaaggaat aaaaaactta
    28681 tatcggaagc tgacttaaga aaaccaacta taaatggagt taaaaacata ttagttaata
    28741 aaaaaagtat aataacacca ttagctgtag attttatgag aatacaccat ctcaaactaa
    28801 agaaactgta aggaggtaat tttatggata taggaaaagt tgtaggtaat gtttgggcta
    28861 caagaaaaga tgaaaaatta tgtggccaaa aacttttagt tgtaaaaatt ttaaaaacta
    28921 aagacacata caaggaaggc ttcatggttg cagccgataa tgcaggagca ggaaatggag
    28981 acttagttct tatatccaaa ggttcctcag ctagagagtc gattgaaaaa tctcatgttc
    29041 cagtagatgc aactatagtt ggagtagtcg attcgtttga ggtgtttgat gaataaaagt
    29101 ataggagcta tagagtttaa aagcattgct aaaggtatag aagtatccaa tgaaatgata
    29161 aagaaatctt cagttgatgt attgtactta aaaagtattt gcccaggtaa atttctaata
    29221 atagtaggtg gagaaacttc ttatataaat gaatgtgtag attatggaat aaaacttgga
    29281 gaaggatata ttgtagataa cttcgtaata aatgcaattt cacaagaaat attagatgga
    29341 tttaaaaata agtatcaaaa gctagatagt atagtctcta taggcgttgt tgaaagtagt
    29401 aaaatatgcg ctggaataaa aatgcttgat aagactttaa aatctggcga cttagtatta
    29461 gtaaaacttc aattatcttt tgctataggt ggcaaattag tatatatagt agcaggagat
    29521 ttaagtagtt tagaatatgc attaaaagaa agtgaaaatg tagtaagaga aaaagaagta
    29581 atatataaga ctgtaatacc atcagtagac agtcaaataa taaaaagtct aataaaataa
    29641 tggggggaat tatgagtata aacgaaatta taatctatgt aatggtagtc ttcatggtac
    29701 ttggagctat agataaatgt ataggtaaca aatttggtct tggagaacaa tttgaggaag
    29761 gtataatggc aatggggtca cttgcagttg caatggttgg tgttatatgt ttagcaccag
    29821 ttcttgctga tgtattaaga ccaattgtag taccagtatt taatatactt ggagctgacc
    29881 cagctatgtt tgctggaagt ttattagcta atgatatggg tggtgcgcca cttgcacttg
    29941 aacttgcgaa agaccctaat gctgggttat ttggtggatt aatagtaggg gctatgatgg
    30001 gacctacaat agtatttata ataccagttg cgcttggaat aattgaaaaa gaagaccaaa
    30061 aattcttagc aacaggaata cttgcaggta taataacaat acctattgga gcattcgttg
    30121 gtggattagt tgcaggattc gaggtaatga tggttcttag aaacttagtg ccaataataa
    30181 tatttgcagt agttatagca cttggattat taaaattcga aaatgctatg ataaaaggat
    30241 tcacatactt tggaaaaggt gtagtaatag ttataacttt aggacttgct gctgccatag
    30301 ttgaagcttt aacaggaata gttgtaatac ctggaatgaa accaatcagt gaaggtatag
    30361 aaatagttgg agatatagca atagttttag caggagcatt cccattagtg tttgttataa
    30421 caaaggtatt taataaacca ttaatggctt taggaaaagt tcttggaatg aatgatgtat
    30481 cagcagcagg ccttgttgca agtttagcta actgtatacc aatgtttggt atgatgaaag
    30541 atatggacaa tagaggaaaa ataataaatg tagcattctc tgtatcagca gcattcgtat
    30601 ttggagacca cttaggattt acggcaggat ttaattctaa tatgataaca ccaatgatag
    30661 ttgctaaatt agttggtggt ataacagcag tattgttagc tatggttatc gcaaataaga
    30721 cactaaagaa ggagaatgta taagatggat atatcaaata tagataaaaa tttaattgaa
    30781 acattagtaa gacaaataat agaagaaaaa ataagtggaa ctaaagacac agtagatttt
    30841 gttagaaata aagacattag tggaataact tctataaagt taccaactgt aaaagttagt
    30901 gaatctgata gattagatac agggaatcca agtgatgtag tttacacaaa agatttattt
    30961 acattagagg aaagcccaag attaggttgt ggtatgatgg aaatgaaaga aacaactttt
    31021 gattggactc taaattatga tgaaatagat tatgtaatag atggaacttt agatataata
    31081 atagatggaa gaaaggtttc tgcttcttca ggtgaattaa tattcatacc aaaaggaagt
    31141 aagatacaat tttctgtacc tgattatgct agatttatat atgtaacata tccggcagat
    31201 tgggcatctc aaaattaata aaaatcaagt taagttaata atcaaataaa taatttaaaa
    31261 tacaaactct taaataagca cgcatattta aaaaagctat gaattaaaat tcatagcttt
    31321 ttttataaag aatgttatat tttagacgta ttttttctat atttactggg agatatatta
    31381 tattttttat taaataatct aataaaatgc tctacatttg gataaccaat actattttac
    31441 tgataatctt ttccttatca ctaatcttac attttaaaat attattttta ctcgtaatat
    31501 tatcataaga atttgtttct tatttaaaac taattagaat tataaagttt attgccatgt
    31561 tattaataaa ataaatccag ttaaatcgag tgctagttaa agtaataaga aacttttaca
    31621 atggtttcct ttatcgattg aaataaaagt taatatggta taatatctaa agagagaatc
    31681 ttatcaatta gaatatgcat agaaacaagt ggcttaaaag gagacgttat atggaagaga
    31741 tagtaaagaa ggaacaagca agtatgaatg atattttttt gtatcttaaa gctaaatcaa
    31801 actcattata taaatttgta atattatata atgagtttat caataaacct aaagattatg
    31861 gaactgggga acttataaac atggttgaag tacatatgct aacagcgatt gaagaaaatc
    31921 caggaataag tccaactcag ttagctaaaa tatggaacag aactaaaggt gcaatatctc
    31981 aaactatatc taaattggag aaaaagggtt atataactaa agaaaagaaa gaaggaaatg
    32041 caaaaactat attactatat ccaaccacat caggagtaga gttaagtaag gcacataaac
    32101 tatatgacac catagatgtt acaagaacat ttgaatactt aatcaaggag tgtacattag
    32161 aggagataga tagtttttat aaagtaattg atttgtttat taatttatta gaagaatctc
    32221 cgcaggaaaa taatgattct gaaaaagtgt agctgagatt gaagttgaat aaagagaaat
    32281 agatattata gaagtagtac ttaagtacac attatataaa aatataatag gtataggatt
    32341 aaaaagttta gtggtttaaa gtttaaccac taaacttttt taatgtctat aatctactat
    32401 tatttaattt actaaaaata aaaaaactca ttatattaag atagaaatag aaaaataact
    32461 agaaaaatac tataatatat cagcttaata cagaaaaaag ttaaaaaatt tgagaaaaat
    32521 aaaaatgaaa acgttttata ttgacataga gaaaaagtag tgataccatt taaatatagt
    32581 ttagtagcta aactatattt aaattataat aactatttta gggggatgtt atgaatttaa
    32641 aacaattaag tgaaaaatac gaagaatatg taattcaaat gagaagagaa tttcataagt
    32701 atccagaatt atcttttaaa gaaataagaa caacccaaag aataagggaa gaattacaaa
    32761 aaataggggt cccttttgta tctattgaac caaatatcgt aatagctact atacaaggca
    32821 aaaatgaagg taagacaatt gcaattagag gagatataga tgcgcttcca atgcaagaag
    32881 attcagatgt tgaatacaaa tcagaatatg atggaataat gcattcttgt ggacatgatg
    32941 ctcatggagc tatgttacta ggacttgcac atatgctaaa tgatataaga aaagatttaa
    33001 aaggtagagt attattagtt tttcaagcag ctgaagaagt tggtggtgga cacaaggaga
    33061 taatacagta ctttaaggaa aatggaggac cggatagatt aatagctact catgtttggt
    33121 ccgatattga agctggtaaa atttctgttg aaccaggacc tcgtatggct ggtgggtctg
    33181 gttggagaat agacataaca ggtagaggag gtcatggttc tagaccagat caaagcatag
    33241 atcctataaa gccagcttgt gatatagttt taaaagtatc ttctatacca gttaatcata
    33301 taagtgtact agaacaatgt gttgtacatg catgtgcaat acatggagga actacaatag
    33361 gaaatgttat tccaaacaat gcagaggtaa taggtggata tagatatttt actgaagaat
    33421 caggcaaaaa agtgtttgac attataaagc aaatgagtaa tggtgttgga caagtttatg
    33481 gagtggatgt gaaggttact catactggag gcataggacc tgttatcaat gatgaagaag
    33541 cagttaagat ggctaaagaa atagtttcta atatagatgg attggagtta gattcttatg
    33601 agccaatatg tgcatcagat tgttatggtg aaatattaaa agagtattct ggcttttatt
    33661 gttatctagg ggtaggtaac aaagaaaaag gaataatata tgcacaacac catcctaagt
    33721 acaatataga tgaagatata ttaaagttag gttgcgaatt tatggctaaa tatgcagttg
    33781 aatttttaaa taaaaataat aactaaaata aatctaattt tataaatggg gggatgaaaa
    33841 tgcaaggtgc attaaaggaa aataagaaaa aaagtggtgg aggattttta ggatggattg
    33901 aaaaagttgg gaataaaatg ccacatcctc tagcattatt tacatatatt acactgatag
    33961 tagtagcatt atctgcaata gcaagctttt taggtttttc agcaacaagt cctgctacag
    34021 gaaagttaat aaaagttatg aacttaataa gtgcaaaagg tttagtacta tttatgcaag
    34081 agtttgttaa gaacttccaa aacttccctg tgcttggagt agttttagtt atggcagttg
    34141 ccacaggagt ttgtgaaaag acaggatttt tttcaactgc cataaaaatg agtttatcaa
    34201 aagttaaggg gaatgcggtt gtatttataa tagcttttat aggagtattt gctaatcaag
    34261 ctggtgatgc tgcttttgta ttagtaccaa ctcttgcagg agcaatattt tatggattaa
    34321 atagaaatcc attagctggt atattctgtg gatttgcatc agttggtggt ggatttgcaa
    34381 cagctgtaat tcctggggga tgggacgtta cgctaacgcc aataactgta tcagctgctc
    34441 aaactattca acctgcattt gatatgacat tattagctag ttattatgca ctatttgtat
    34501 ctgcgtttat agtagctaca gtatcaacaa tagtaacggt taaatttata gaacctaaat
    34561 taggtaagta tactggtaaa ccagaaggta tagatgataa tcaaggtttt gaggtcacaa
    34621 aagaacaagc aaaagcagca aagttagcag gttttacagt tttagcttat gtagcgttat
    34681 taattgcttt gtgtatacca gctaatagtt ttttaagaag tccagaaggt tctttaatat
    34741 ttggagcacc acttatgtct tgcttacagt tttttatagt aatactattc ttcttgccag
    34801 ggattgtgta tggaatgagg gttggtaaaa tcaagactgt aaaagactta aatgatatat
    34861 tatgtcaatc aatggcagca atggcaccgt ttatcgtact tgcaatagtt ataggtcaat
    34921 tcttaacgct attctcagtg tctaatttag cacaggtact agctataaaa ggtggagcat
    34981 tactcaactc attgccacta ccaccacaag ctataatact gctattctta gtactagtag
    35041 cgtttataaa tatatttgtt ataagcggaa gtacaaaatg gatgatattt ggaccagtat
    35101 ttgttccaat gttaatgcaa ttaaacatac atccagcatt tactcagttt gtatatagat
    35161 taggagattc tgtaaccaat catttatcac cattaaatgc attctttata atacttttag
    35221 caacagtcca aaaatacgat aaaaatgcag gtatgggaac tatattctca gctatgatac
    35281 cttacactat aggttacttt gtagtacttg cagttttagt agtagtttgg atgacaatag
    35341 gtataccagt aggaataggt ggacaagttt ggttataaaa catatttaat aattttggga
    35401 ggaaaaaaaa tggaaacaac tgaaataaga tacaattttg atgaagtaat agatagaagt
    35461 ggaacaaact gtcttaaata tgatcttaag agtcaattta tgccagaatc tccagaagat
    35521 gcactaccac tatgggtagc agatatggat tttgcatgtg cccctgagat aattaatgca
    35581 atgcatgaaa gaatagacag aaaaatattt ggatatagct ctcaacaaac tgatgattat
    35641 tatgaagctg tatgtggatg gtttaagaga agatttgatt ggaaaataga aaaagagaat
    35701 atattcttta gcccaggggt tgtgcctgcg ttaggatttt tcacacaaat attaacacaa
    35761 aagggtgatg gtatcataat agaaaagcct gtatactatc cttttgaaga taaaataact
    35821 aacaatgaaa gaaaagtagt taatagtcct ttaaagtttg aagatggtca ttattctatg
    35881 gattatgatg gatttgaaga attagctaaa gatgaaaata ataaggtgtt tatattatgt
    35941 agtcctcata atccagttgg tagagtatgg aaaaaagaag agcttagaaa aatagtagac
    36001 atatgtaaga aatatgattt gtggataata tctgatgaaa tacattgtga tattattcgt
    36061 aaaggtgaaa agcatatacc attagaagta gcatgtcctg attataaaga tagaataata
    36121 acttgtacag ctccaagtaa gagttttaac ttagctggta tgcaattatc aaatatagta
    36181 atcaataata aggaattaca agaaaaatgg atgcttgaaa cagattctaa attagctata
    36241 ttcctaccta atccattcgc aatagttgct actaaagttg cttataatga ttgtgaagat
    36301 tggatgaatc aagtaaatga gtatatagat ggtaatatta aatatgtaga agattttgtt
    36361 aatgagtata tgcctaaagt aaagataata aaatctcaag gaacttactt agtttggtta
    36421 gactttagag ggtatggatt agatgcaaaa cagcttgaat atgtaatgct taacaaggcg
    36481 aaggtattgc tagatgaagg atacatattc ggagaagaag gagcaggatt tgaaagaata
    36541 aatgttgctt cacctagaac aattatagaa gagtgtatga atagaataaa atgtgcattt
    36601 gaaggattat aatagtaata tctaaataat atatttgtgt attttaaaaa aagactatgc
    36661 taaaaaaata tttcaaagca tagtctttta aagttttatg atgagtttaa aaaatttcaa
    36721 aacgttacta ttcacttata tgtttatatt agaaataatt ccacaatata ctcattttct
    36781 aaagttgcac ttttatacac cttgcttcac caatatcatg tgtaaccaaa atagtagact
    36841 tattctcatt cttaataata ctataaacat catcacaaac taataatcta gtctgataat
    36901 caagagctga aaaaggttca tctaaaagta gaatatctgg atttacagaa agtgttctaa
    36961 taagcgcaac tctttgtctc atacctccag ataattcttt tggatacata cttctaaaat
    37021 cccataatcc ataagtcttt aaaaggcttt caactctatc taaagatttt ttatcttttt
    37081 tgccttgaat ttccaatcca atagttatat tatccattat atttctccat tcaagcaaat
    37141 tatctttttg aaacatatat ccaatatttc cagtaatctt tatatcacca ctagttggtt
    37201 ttaaaagatt agtaagaata tttaatattg tagatttacc acttccagaa ggaccaagca
    37261 aagttaatat ttctccttct ttcaagttaa aattgacatc ctttaatact tcaagctcgc
    37321 cttccttagt gtagaagttc atatttatat ttttaacttc tactatgttg tcattcatta
    37381 tgccaccccc tataactttt agctcgtata tattatgtta ttaacaatat gacctatttg
    37441 taccattttt cataaatata atataaatat tttgtttatt tacgtatttt cttagtaact
    37501 tgtacgtata aattatagat aaataaatta taaggtaggg gataaatttg agtaacataa
    37561 taacgaattt aatagagtca atatttacta atccaataac tatattgttt ttacttgcaa
    37621 taactgtgta ttctgttttt aaaataatag aaaataacag agtatattca tttgtaagtt
    37681 caaaattaga cgaaatcaat agagactata agacaagtga gttttataaa aaggtaaggg
    37741 atgactacta tacatatagt aaagaaaatc cttatgcaga cgtaaatata acttcattta
    37801 tagaggaagt tgtatctgat ttaaagcata ataatttgcc attgttagaa aaaattagaa
    37861 gtataaaaaa ttcatcttca ataagtatat tacttggggt tttaggtacg tttgttggtc
    37921 tttctacaat gcttttatgt gttgatacta aagacattat aaattcactt ccttcaacta
    37981 ttagtagtat gcaaactgca tttacaacta gtatatttgg agttgtattt tctctaatta
    38041 taggttattt tactaagata aaagattgtg aacatgtttt gatacaaata atgttaaaat
    38101 ctgaaaattt actaacatct gaaataactc attttaagtc agaaagaatg gatttaaaag
    38161 tagaagaggt aaaaaataca ataaaacaaa taagtaagtc tatagaagct attgaaaaat
    38221 ttgataaaat atctaaagat ttaaatgact ttaatgatga atttatcagt ggtatagaag
    38281 ctttaaaaag ccttttagaa ggttctcaaa gttctattaa gacatttgac cagagcgtaa
    38341 gaaaattaga taaacagttt aatattttaa atataaaatt tgtaaagctt tttgataaat
    38401 atgataatca agataatata aataaagaga ttttatttga tataaaagaa tcttcaaaaa
    38461 atatatacaa tgccactgaa agccaattca aaattagaga ttatataaaa aatataaatg
    38521 caggatttgc tctatatgaa agaagtgctc aagatttatt aactaaatta atgacccatg
    38581 aaaataaaat ctctcaaaat caaaaaatcc ttttagatga aaagtttacc ttagatgact
    38641 caataaaaaa tctctcaagt attattgaaa acttttctaa tgacctacaa gttaaactgg
    38701 atatgatgtt tgaaaattct ttagatattc aagataagtt agatgttatg tttaataact
    38761 cttttatgaa tgatgaagtt cctcttgata gtgaagaatt atttaaggat gatgttaata
    38821 atgtatttaa tccttttagt gaagaaattt atgagataga ggataaagaa ataaaggtca
    38881 taggtgagga tgagttaaat gaataataaa tatagaagaa ctgttaacag agaatttgaa
    38941 aagagtagct tttggccaac atttacagat ttattatcta ctgtattgat ggttgtaatt
    39001 cttatcttat ttagctcaga aagtatttca ggttctgttg aacaagattt agctaagaat
    39061 gtcaatgctt ctgttgaaga aacttttaaa aaaagtggaa tacctgtgaa ggtagataaa
    39121 tctaatgggc aagtaacttt tggtgaaaaa accatgtttg atgtagatag tgatgtttta
    39181 aaaccagagg ctaaagaaat gctaaaaatg tttgttccaa agtacataga gactatttat
    39241 aaagattatg gtgactatat atccaaaata gttatagaag gtcatacaga tgatgtggga
    39301 agttatatat acaacttaga tttatcgcaa agaagagcat atagcgttgc taaatttata
    39361 gtgggagatg aaataggtga ctataaatac aaagataaag tgactaagca tattattgca
    39421 ataggaaggt caaaagctga actcatcaaa aatggtgaca atagcgtgaa tagagatgca
    39481 tctagaagag tagaactaaa atacgaaata aatattaatc aaaataaata attaaatttt
    39541 tataaaaaaa aagaaggcag ttggtgtgag ctttaagcct tctttaagga aaaagtttat
    39601 ttatttttgt tgatacttaa catattatag tttttagaag cagtttgcaa tatattaaac
    39661 gtaaatggtc gtttaaaatg cataactgga tactactgat gtaaaatatt tcattttata
    39721 taatataaaa attatacagt atcaattttc tatattattg tactataatc tattatttaa
    39781 aatattatgt aaaaaaatat cataaaaacg catatcatgt tataattact agagtattat
    39841 gaatatggag gcgtagtttg tgatagaaat aagtataaaa aataaaaaaa attctaaaat
    39901 taaaactttg atacttttat tgatagtatc tatatcaatt tatttaactg gttgtactac
    39961 attagataat ggaaataatc aatcaaataa taatgattct ttgtctagta gtatatcaag
    40021 tgataagaat gtaaaaattc attttattga cactggaaat agtgatgcaa tattgattca
    40081 agatggtaaa acttttatat tgatagatgg gggagataat gatgatgagg gattaatggt
    40141 agattaccta aacaatcaag gtgtaaaaga tattaaatat cttatagcta cccattctca
    40201 tgcagaccat ttaggtggac ttgattctgt tgtcaaaaac tttaatatag aaaatgtttt
    40261 tgtatcgaat ggttcagcag aaacaaagag ttatagggac tttataaatg ctttagcaaa
    40321 taagggttta tccccaagtg taccgcttga aaataataag ttctatttag aagattctta
    40381 ttttgaagtt ctaaatacaa atgggggaga tactactaat gaacaatctc tagtccttgt
    40441 atatactaat gggaatgata aagttttatt tacaggtgat gctgaagaag gtaccgaaaa
    40501 agaaatactc cctaagcttg agaaagtaga ccttttaaaa gtagctcatc atggttctcg
    40561 tagtagttca tctcaagagt ttttagataa agtaaatcct gaatatgcgg ttcttttagt
    40621 aggcaagggt aattcatatg gtcatccaca tcaggagaca atgaataaat taaaaaaaat
    40681 gggagtaaaa gtacatagaa gtgatgagtg ttcagatata atatttgaat caacaggtga
    40741 tggagtattt acaagttgta aagatggaag ttataataaa ggcgttagag aagatggaaa
    40801 ctctggaaaa tcaaacagta atacaagtaa aaatgattct tttaaaaata aacaaaatac
    40861 atccaattca gaagaaatag tgtattttac tccaaaaggt aagagttatc actctacaaa
    40921 aaattgtagt gggctttcaa gaagtaaaaa aatattatca ggcacaattg cagaaagtaa
    40981 gaaaaatgac ccatgtgata tatgttatgg aaaataacaa aaaaacaacc aatttatcta
    41041 taaagcttct gaactttaaa tcatttaaaa aataaaataa atgcataaaa aaataagcta
    41101 taatcaatag ataacacatt ttatctatac gaatagctta tttttttaag tattttttaa
    41161 tagctattta ataactctaa atacaccagt tacaatacct acaatttcaa cttctgagtc
    41221 attgagcata attggttcca taaaatcatt ttctggctga agtcttatta aatttccttc
    41281 tttaaaaaac ctcttaactg tagcagattc accatttata agtgcaacta ctatctgaga
    41341 atttaaggct gtattttttt tgtctactac aacgtaatct tcatctaaaa ttcctgcatt
    41401 aatcatactt tcgcctttta ctcttaatac aaaattgtta ctgcccttta ctaaactggc
    41461 agggaatgga atgtattcct ctatgttttg ttgagctaaa atgggttctc cagctgttat
    41521 ttgacctact aaaggaagct ctattatttc ttgattagct ccagaaacat catttacttt
    41581 actacgttct aaaacttcaa tagctcttgg tttagtaggg tcctttctta tgtatcccag
    41641 tttttcaagt ttatttagat gagagtgcac agttgaagta gatcttaacc ctacagcagt
    41701 acatatttct cttacagcag gtggataacc ttttaatatg atttgagact ttataaattc
    41761 caatatcaag acttgctttt cagttagatc taaatacata acatcactca tttccaaaca
    41821 tattttgttt ttatttccat acactttata tttttaatta taacataatc catttatatc
    41881 tgcaaacatt tgttcctgat ttttaatcgt cctcaatcca ttcaaaattc ttgactatat
    41941 cacgttttac atttttctta tgagctgcat aaagctgagt agttgtgaca ttatcatggt
    42001 caagtaagtt ttgaacatca tatatagaaa atcctgtttg gattagagaa gttgcagccg
    42061 ttgctcttaa tttatgggga ctatatccat tgtctctagt agtatcaagt ggaatagatg
    42121 tatatttttt taccagtgtt cttatagcct ttgctgtaat tctcttattt tgaagagaca
    42181 aaaataaagc atctttttct tcatcattta atagttcatc tcttgttctt tcgttttgca
    42241 aataatcttt tattacatgt tcacaagtat gatttatagg cattaaaact tctttcccac
    42301 gttttctata tattttaaat tcacctcttg aaaaattaaa agaggagata ttgagttctc
    42361 taagctcatt taatctaagc ccgtatgtaa caaataatgc tagtatagct ttgtcacgta
    42421 atttagtttt tctccaatat accttttctt tctctgtaag cccttcacct gtttctactg
    42481 attcaagcat cttagcaact tcatctatct caaggcgctt aatagcatct ggctgaggtt
    42541 taggaagttt tattggattg aatccatcag ttatattatt atctatctga gagtttctgt
    42601 ataagaattt aaatagagta gagatggatg atttttttct tgctaaagct ctattgttat
    42661 tttcaaaaat caatgtgttt ttttctgtat ttttgtaata tctactgcaa taatcaccta
    42721 agaataaatt tatatctctt gattttattg tattaaaatc atcttctgta atatctttta
    42781 cacaatctgc atttgttaat tctttggttt caatcaaata tgaacaaaaa aaattaatat
    42841 cttctaaata agcaagtcgt gtagaaactg caacagaacc ttttaaatat ataaaataat
    42901 ccttcataaa tacaggaagt ttattttcca aaagtagaca tttttctatg agttcatttt
    42961 ctttgataac aatattatca ggtttatctg atttattatg tacttttata attttcttat
    43021 cattattcaa aattaaacct cctaaaacta aaatataaat cttttatatt attttttgat
    43081 atttatacca agtgagtata aatatttaat ttaatcattt tttacatttt taagagatta
    43141 gcagtgcttt agatgaaatt atcactaact attccctaaa tccacattta aagactagtt
    43201 gaatttaagt actctttttt atttctcaat atatttatag aaatatttta aaatatattg
    43261 agaaattttt tatgctctac taaaatcttt tttatataat atttttatat gtttctaacg
    43321 aaaccaaata ttagtaaaat atgtatttat ttaactaaag tttttaaatg ggctgactaa
    43381 gcttaaaata taggatatta tgagagttac tattgaaaaa ataaacccta ttgccataaa
    43441 gaaacttata aatatgttat acttaactat taaactggat atattcaagt tatcaaatcc
    43501 aaataagtat gtaattgaac ttattacaaa aataactata actataaagc tcagaccatt
    43561 taagctattt tttaaatcag cactactaag tgacatatgt gtagatatag aatacattaa
    43621 aaacataaat ataaaatact tcaatgaata tacactaggt gttaacaaca ttattcttat
    43681 aaaattggaa atatcaccta aaataatgtg tataaaccca tttatattta aattattaat
    43741 catacttaca tataacttta aatctatatt ttgagtcact ttcatataac tatctggcag
    43801 taatatctta aaaattaaaa taatcattat tgttccacct ataataggtg caattcctat
    43861 aaagaaattt cctatacttt gataaagatt atctttttta tagctatgac ttacatatcc
    43921 tagtatacca tcatttttac tttctattgg tctgaaaaac ttaactgaat ttattttatg
    43981 catgaaaatt aaacacatta tcaaatgact caattcatga acaaacgttc ctatagctcc
    44041 agttacaata attccagttt ttccaaaaga tgaaaatata aatttattat ttagattttc
    44101 tgtaatgcta aaaatcaatc caaatacaat aaatattcct atgaaacaaa aaatatttat
    44161 tacacttatt tttaaaattt cgattatata aatcataagt taagtcctca tgtaaaagta
    44221 tttaactact ctacttcata tccattacag cttaaaattt tacaagcatt ttctatatct
    44281 ttttctttta ctaagatata gtctgtatca taagtagaaa ttgcaaatat gcttattttg
    44341 ttgtctgcta acaaactact taacttagat aaaattccta caagtgaaaa gtctaaagga
    44401 cctagtatct taagtattct ccactccttt tctgacttga catcagaagg aatattatta
    44461 tttaaacata caacacttaa ttcatcatca gttctagtta tagagtaaaa cttctctgta
    44521 tctatccatg ttggtatttt actgtcatta tttaatctac atactgcata ctcttctacc
    44581 aataatttta atttcataat gtatatttcc tccaatctta tttgtttact taagattata
    44641 cattaaaagt tacataaaat aaaacatata aagtaaaaaa ggttcactat agtgtcctat
    44701 ttctatagcc aacctttaaa acttaattaa attctaataa agagatatat tatctaggtt
    44761 caattataag tttaatggct gttttcttat caccttcgat ttctatatca gtaaatgcgg
    44821 gaatgcaaac taaatccatt ccactaggtg caacaaaacc tcttgctatt gcaattgatt
    44881 ttatcgcttg atttagagct ccagctccta tagcttgtat ttctgcaacg cctcgttctc
    44941 ttagtactcc agccaaggct ccagctacag aattaggatt agattttgat gaaactttta
    45001 gtacctccat ttcaacatct ccttgttaat ttgttaaagt atccattacc taaagtacta
    45061 aatcataatt gccatttact aattacaatc atcatttaaa gtatactttt ataaaaatat
    45121 acttctataa tcgttttaag aaaaccttta ttttttttaa ccattttatt atgaaaaaac
    45181 tacaaatttc tttatattct ataagcattt tccaatattt ctaaattttt tatatctttg
    45241 agctataatt tcctctttat ccatatcttt gtaatgattt aatctttcta aaatataagc
    45301 ttttatttct tttgaaacag cttctacatc tttttgtgct cctccaagag gttcttttat
    45361 aatcttatct attattttta aatcatataa atcattactt gtaagcttca taacatcaca
    45421 agcttcttta gctttagatg aatctttaaa tagaatacta gaaagtcctt ctggagaaat
    45481 tacagaataa actgaatgtt ctaacataca tacatcattt cctactccga tacctaaagc
    45541 tcctccacta ccaccttcac caattacaaa agtgattact ggaacagaaa gtttactcat
    45601 ttcaagtaag tttcttgcaa tagcttctcc ttgtcctctt tcttctgctt ctagaccaca
    45661 aaaagctcct gatgtatcaa taaacgttat tataggtcta ttaaattttt cagcttgttt
    45721 cataagtctt aatgcttttc tgtatccttc tggatgaggc attccaaaat ttctttttat
    45781 attttctttt gtatctcttc ctttttggtg tccaactata gtaacattaa actttcctat
    45841 ctgaccaatt cctccaacca tagaagggtc atctttaaaa agcctatctc catgaagttc
    45901 taaaaaatta gaacaaatat gttctatgta ttccaatgtc gtaggtctct ttatatctct
    45961 acttaatgtt accttttcat atgctgatag atgtgaaaaa gcatcttcct taagcttttc
    46021 caatttctca ttaagtgaat ttattttatc acttaaatct atattatttg ccttagaaat
    46081 agttattaat tgtttaatat cattttctaa gacttttatt ttttcactat tattttcaat
    46141 caaaacagtt cctcctttat gccctatttc ttgtgcatag caagaatttg atataatact
    46201 tccttcattt ttcttctatc tactatcatg tcaataaaac ctttttcaag taaaaactca
    46261 gaagtttgaa atccttcagg aagtttttga tttattgttt gctctattac tcttggacct
    46321 gcaaaaccta tcaacgtatt tggctcagaa attataatat caccaagcat agcaaaacta
    46381 gcagttactc ctccagtagt agggtcagtt agaactgata tatatggcaa taatttttct
    46441 tcaagcttag aaagtgcttg agaagtcttt gccatctgca taagagatac cataccttct
    46501 tgcattctag ctcctcctga agcagaacaa ataattatag gtaaattatt ttcagcagca
    46561 tactctattg aataagttat tttttcccca acaatagaac ccatacttcc catcataaaa
    46621 tcaggattca ttacacaaat aactgtttta attccattta ttcttccata acctgtgaca
    46681 acagcttctg actcttttgt tttttcctta taagagtcta ctttttcttt gtatttagga
    46741 aaatttagtg gattttgaaa ttctacatca gaattaaact ccataaaagt tcctttatca
    46801 atcaataaat ttattctttt tcttgctctt aagccataat gtcttgaaca ttttggacat
    46861 acaaatgaat tttcttctat gtcctttcta aatacatgac tatcacaacc tttgcaatag
    46921 gtccaaaatt tatcatctac actattcttt ttaaagttgt catctaaaga aatcgtaaca
    46981 tacttagatt cttttgatga taaaaatttt tttatcataa tctatttact ccttataatg
    47041 tagcaaattc tttcttcata aagacatctt ctataaattt ggtatgatga gtatcttctc
    47101 taaactcctc acttgttaca agtgcctttt gaaattctac atttgtatta ataccttcta
    47161 caataatttc gtctagagct ctatatattc tacttataca ttcttctcta gtttcagccc
    47221 aacatattaa cttacctatc atagaatcat ataaaggtgg tatagtatat cctgcatata
    47281 caaaagtatc aaatctaaca ccaaaacctc caggtaaatt taatgattct atcttacctg
    47341 gtgatggagc aaaattcttt gatgaatctt ctgcatttat tctacattca atagcatgac
    47401 ctctaaaagt aatatcttct tgtttaaaag gaattttatc cccattagct atagatattt
    47461 gaagttttat aaggtcaaga tttgttacca tctctgtaac tggatgttca acttgtattc
    47521 ttgtattcat ctctataaaa taaaagtttt tatctttgtc aactataaac tcaatagtac
    47581 ctgcaccgat ataacctact ccttttacta tatctactgc aattttatga agtttttgtc
    47641 tttcttcatc tgaaaggtat ggacttaaag attcttctat tactttttga tttcgccttt
    47701 gcatagaaca atctctatca cctaaatgta ttgcatttga atgtttatct ccaaatactt
    47761 gtacttctat atgtctagga ttttctataa acttttccat atataaatca ctattagaaa
    47821 atgctgctag agcttctgaa cttgccatat caaaatttga aaaaagttct tcttcttttc
    47881 taacgattct catacctttt ccaccgcctc cacttgaggc ttttatcata acaggatatc
    47941 caatttctct tgcaacttct aaagcttctt ccttagtttt caaaacagat tctgaaccag
    48001 gaacaacagg gatattcaat tccatcatag tttctcttgc tctagattta tctcccatca
    48061 aaatcatttg ttctcttgtt ggacctataa aaataatatt attatcttca cattgctttg
    48121 caaattcagg attctctgat aaaaatccaa agcctggatg aatagcatca cactttaatt
    48181 taagagcaag atagattata ttttctatat tattataact cttacttata ttatttggtc
    48241 ctatacaaat agattcatca gctatatatc tatgaaaaca atctttatct atttcagaat
    48301 agatagcaac agtttttata cctagttctt tacatgttct gatgattcta actgctatat
    48361 cgcctctatt tgcaactagt atttttttta tcaacttaaa tacctcctac tacaatggtt
    48421 ttatcttaaa taaaggttga ttatattcta ctagttcttc attactaact aatacttcaa
    48481 ttatttcacc tttaatttca cttgttatct cattcataag cttcattgct tctaatatac
    48541 ataaagtgtc gccttcttca actacatcgc caactttaac aaagctatct gcatctggac
    48601 ttggagaatc atagaatgtt cccatcaaag gagctactac tactgataga ttctcattag
    48661 atgtgacagg tgtatcgttt acaactggct tttctaaaga aagatccact actgattctt
    48721 gttgtacact taaaacaggt gaacttgtag attgagcttt tttagatact tctaatctta
    48781 aatcgctact ttcaagtttt acatattcta agttagttga gtctatggcc tttaataatt
    48841 ccttaatttc attgatgttc attttgttac ccccttgtat tattacctgg tgctaatatt
    48901 atatcacaaa aaacaaattt ataaaagaaa aagtgctgac tagagaatca acacatcaat
    48961 atatctttta attttaaatt aacaaccagt aacttatatt tgttttaatt ttccattatt
    49021 aatataaatt ataagtataa aatgaataaa attcaatatt ttttgcaaaa tacaagagaa
    49081 tacaaattaa tacctcggga atttttaaga caaataccaa tattatgcaa tttagacttc
    49141 gctttctttc aaattagtat cattttcttt atttttacat aggatgtact ttaatttagc
    49201 tatcaaaaca ttgctatata ggttaaaagt tattagaatt gcaggaatta taaagtaaac
    49261 tctagcacct ataaaaccaa aaactactgt aataagtatt aatcttttat ttaaagtaaa
    49321 aaaaggtata ttaaaaatac ttttgtcgga tgtttctgga gtataagaat atataactcc
    49381 aaaaatcaaa gtaagcatac aaagtggaag tagtgtataa cttgaaatta ttcccagaaa
    49441 tcctatactt tcaaaatatt tgctagcact atttgtataa acaaacatgt atccatttaa
    49501 atatgcgcat atacatgata taatgccaaa agttccccct aaggattgag tgtaccaatt
    49561 aaaaaattta gattccatat acctcacctc ctaaaactgt atatataatg atatgcattt
    49621 aaatagtaaa atatatcttt atatgtttct gaataaaatt ttgttaattt cattaattta
    49681 tgtttaagaa atttgaagta tagtgtagta tatattaatg tagatgaagt tttatagtac
    49741 ttatttcata gtgcttaatc aagttatctt attatggatt caaaagttgt ttgtagggtt
    49801 ttatattttg ttgagctgaa aaatttatta taaatgaagg gatgttttcg ctttggtaga
    49861 tacattagaa tttggtctta aaattttatt ttttatatta tcaataattt ggatgggtaa
    49921 aataatgatt ttaagaactg ataagcaaat agtaatcaat cctttgctta ttggtatatc
    49981 tgcagtcctt gtgatgttgc atacctcaca gagtaacatt gaattttttg gattagatgt
    50041 ccaatatata agaatagttt tgtatataat atattcactg ataattttaa taggaatatg
    50101 ggctactaac agaagaaatg gaatttttta gataatctat ttctataaaa tttaaaggtt
    50161 gctagtaaca aagcactaat attagcgttc tgttactaac aacctttatt ttgattatac
    50221 acttaaattg aaaatagaat gattgaataa gttgacagat tattttacat tgacagtaat
    50281 tagaacttct tgagatgtat ttaaatcatt tttgtcgctt agtataactt ttaccactgc
    50341 ttgacctgtt ttattacctt taattatcat atcttcacat gaaagtacac tcttatcact
    50401 tacagataca ataaaattta aatcttttgg aaggttatca gtaacttccc agtatttata
    50461 ataactatca attttcttcg taattatttt tctcaaatca atactttcac cttttgctat
    50521 atcaacgtta tgatatttag agtccagttt atactgatta tcttttgtat caataaaatt
    50581 taaatcgcaa tttacatcca caccattatg agaaagagaa tttactacat cgtcatcaat
    50641 caaataatta ttggcaagat ttattgaatc catatctttt agacttaaag cttccttagg
    50701 aatctctttt atattattat tccaaaaaat aaaattgtct acattttcaa gatatgatat
    50761 accttttaaa tttgaaatgc tctcaccagt tgcataaaat tctccatcta aagcctttat
    50821 attgcttata gtcatctcat ctggatttat atatcttttt acaactgctc ttaaatttgc
    50881 atcaggaaaa atatctttaa gttgattttc gctcaaaacc ttatcatttg aatttattgg
    50941 acttaaagct gctgcagaaa taataaatgg tattaaatta gctaaattca tagtaccctc
    51001 ccacataaat tttttattat aataatttaa tttctatgag agggttctgt ttccttgtta
    51061 tatataccta ctttcgtaca ctttattaat ataaattaaa ataaatttct actattaaac
    51121 ttataaacat attactagtt acgtttttta gcttcacatc taaatattcg cataccttgt
    51181 gacataaatt tgtcttcata ctctgttgta acattatttt catactcact atttcctaaa
    51241 tcaagagaaa tattctttaa tagccaatta tttgcagcaa tttcatttaa actaaactcg
    51301 aataattttt cattatctgt tttaaagtga atttcgccat catcttccaa aaccctattg
    51361 tacatttcta aaaatccaga atgagtgagt cttcttttac tccatctatt tttaggccat
    51421 gggtcacaga aatttatata aattctcgat aattctttag cttcaaagta atccaaaata
    51481 ttgcttacat ctccccataa aaatagtata ttattcaaat tagaagcatc tgctttttca
    51541 acagccttta aaagaacttc ttccttaagt tccatggcaa tataatttat gtcagggttc
    51601 tgcttagcta aagttgttat aaacttacct tttccagtcc caaattcaac atgtattgga
    51661 ttatcatttc taaactttaa attccacttg ccttttaatt tgtctatatc gtctcttaaa
    51721 acatattttg tataacttaa aagcttttca tctgcacctt tttttcgtct tcttctcaca
    51781 ataattctcc ttacatataa aaaattagta aaatacattt ttattataca ttatctcacc
    51841 aaaaaataaa aagaactctc ttaaaataga aatttatttc tataaagaga gttctaaact
    51901 tactaaaaac ttaatcttag tggcaccctg aacaaccaga gcatcctcct ccaccttgat
    51961 ctttcatatt ttcaggaata actctaaatc caaatcctgt atcttctatt attatatctc
    52021 catactgact atattcatca gagctcatta caaattgtaa ttctccaact tcataagtta
    52081 agtcatcttc ttttttctca tctaaagcta atccaaaaga aggacctcca caaccaacac
    52141 ctgcaaagta tactcttata ttattaggtt tatcttgatt gtcttttaag atacttttta
    52201 aagtatcaat agctgtttct ggtaaagtaa ttttcatcaa atcattccct ttcaaatatt
    52261 ttattaaaac aatatctatt atatacccat acaaggtaca taatcaatta gtttttgcat
    52321 attttactaa aacttctacc taatattacc taaggcttta aaatatttta tataatattt
    52381 tattacctta
//
LOCUS       DAMJWU010000016        49591 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMJWU010000016 DAMJWU010000000
VERSION     DAMJWU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.016"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            245..358
                     /locus_tag="PC460_001112"
     CDS             245..358
                     /locus_tag="PC460_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2600008.1"
                     /translation="MIMGRHLWNPSYFVATVLENTEEQIGKYINNQKKQVK"
     gene            360..1478
                     /locus_tag="PC460_001113"
     CDS             360..1478
                     /locus_tag="PC460_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2600009.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNVLKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNAAISKEPSGR
                     YYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNSKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            1629..2846
                     /locus_tag="PC460_001114"
     CDS             1629..2846
                     /locus_tag="PC460_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF2600010.1"
                     /translation="MLIGLIAAFGLVLQKKKASDVIKGSLLAAFGIIIMETGTGMLVS
                     SIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAIHLMIARFTPIKTIF
                     LTGHMLYWFPFIFVASGVEAGLNGGVLITFAAVLSALYWSIMPWLLRKYVFDVIGDDS
                     FTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREISISGGMAVFLMFIVV
                     GIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQIMPAFQGISEKVVPN
                     ALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILIYNYFNVFNLLLIPLVITSFFECG
                     AAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNWILIFGGNDLSLFGI
                     ISRFIGSLFGGIL"
     gene            2849..3589
                     /locus_tag="PC460_001115"
     CDS             2849..3589
                     /locus_tag="PC460_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2600011.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            3912..5222
                     /locus_tag="PC460_001116"
     CDS             3912..5222
                     /locus_tag="PC460_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2600012.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            5320..6237
                     /locus_tag="PC460_001117"
     CDS             5320..6237
                     /locus_tag="PC460_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2600013.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            6253..7062
                     /locus_tag="PC460_001118"
     CDS             6253..7062
                     /locus_tag="PC460_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2600014.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDTMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            7120..8868
                     /locus_tag="PC460_001119"
     CDS             7120..8868
                     /locus_tag="PC460_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF2600015.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVSTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            9130..10152
                     /locus_tag="PC460_001120"
     CDS             9130..10152
                     /locus_tag="PC460_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF2600016.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            10157..12463
                     /locus_tag="PC460_001121"
     CDS             10157..12463
                     /locus_tag="PC460_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2600017.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            12435..13550
                     /locus_tag="PC460_001122"
     CDS             12435..13550
                     /locus_tag="PC460_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600018.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            13568..14986
                     /locus_tag="PC460_001123"
     CDS             13568..14986
                     /locus_tag="PC460_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2600019.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPNIILPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            15001..16209
                     /locus_tag="PC460_001124"
     CDS             15001..16209
                     /locus_tag="PC460_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600020.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(16283..17671)
                     /locus_tag="PC460_001125"
     CDS             complement(16283..17671)
                     /locus_tag="PC460_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2600021.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            18012..19346
                     /locus_tag="PC460_001126"
     CDS             18012..19346
                     /locus_tag="PC460_001126"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF2600022.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(19488..20405)
                     /locus_tag="PC460_001127"
     CDS             complement(19488..20405)
                     /locus_tag="PC460_001127"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF2600023.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            20613..21497
                     /locus_tag="PC460_001128"
     CDS             20613..21497
                     /locus_tag="PC460_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF2600024.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            21710..23551
                     /locus_tag="PC460_001129"
     CDS             21710..23551
                     /locus_tag="PC460_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2600025.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            23856..24563
                     /locus_tag="PC460_001130"
     CDS             23856..24563
                     /locus_tag="PC460_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600026.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            24557..25867
                     /locus_tag="PC460_001131"
     CDS             24557..25867
                     /locus_tag="PC460_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2600027.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            25984..26667
                     /locus_tag="PC460_001132"
     CDS             25984..26667
                     /locus_tag="PC460_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600028.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            26660..29047
                     /locus_tag="PC460_001133"
     CDS             26660..29047
                     /locus_tag="PC460_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2600029.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAIYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            29327..30469
                     /locus_tag="PC460_001134"
     CDS             29327..30469
                     /locus_tag="PC460_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2600030.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            30488..31012
                     /locus_tag="PC460_001135"
     CDS             30488..31012
                     /locus_tag="PC460_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2600031.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            31112..31930
                     /locus_tag="PC460_001136"
     CDS             31112..31930
                     /locus_tag="PC460_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2600032.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            32008..32319
                     /locus_tag="PC460_001137"
     CDS             32008..32319
                     /locus_tag="PC460_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF2600033.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(32446..34449)
                     /locus_tag="PC460_001138"
     CDS             complement(32446..34449)
                     /locus_tag="PC460_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2600034.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYENMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITYPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDSSI"
     gene            complement(34602..35240)
                     /gene="recX"
                     /locus_tag="PC460_001139"
     CDS             complement(34602..35240)
                     /gene="recX"
                     /locus_tag="PC460_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF2600035.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            35516..36205
                     /locus_tag="PC460_001140"
     CDS             35516..36205
                     /locus_tag="PC460_001140"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF2600036.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            36206..36778
                     /gene="yqeK"
                     /locus_tag="PC460_001141"
     CDS             36206..36778
                     /gene="yqeK"
                     /locus_tag="PC460_001141"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF2600037.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            36895..37245
                     /gene="rsfS"
                     /locus_tag="PC460_001142"
     CDS             36895..37245
                     /gene="rsfS"
                     /locus_tag="PC460_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF2600038.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            37599..40019
                     /locus_tag="PC460_001143"
     CDS             37599..40019
                     /locus_tag="PC460_001143"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF2600039.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            40855..41523
                     /locus_tag="PC460_001144"
     CDS             40855..41523
                     /locus_tag="PC460_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2600040.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(41841..42113)
                     /locus_tag="PC460_001145"
     CDS             complement(41841..42113)
                     /locus_tag="PC460_001145"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2600041.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(42344..42765)
                     /locus_tag="PC460_001146"
                     /pseudo
     CDS             complement(42344..42765)
                     /locus_tag="PC460_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(42784..42882)
                     /locus_tag="PC460_001147"
                     /pseudo
     CDS             complement(42784..42882)
                     /locus_tag="PC460_001147"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            43165..44535
                     /locus_tag="PC460_001148"
     CDS             43165..44535
                     /locus_tag="PC460_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HDF2600042.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            44894..45052
                     /locus_tag="PC460_001149"
     CDS             44894..45052
                     /locus_tag="PC460_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600043.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     repeat_region   45338..45695
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=45338..45366
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     regulatory      complement(45837..45934)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            46160..46543
                     /locus_tag="PC460_001150"
                     /pseudo
     CDS             46160..46543
                     /locus_tag="PC460_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(46732..47727)
                     /locus_tag="PC460_001151"
     CDS             complement(46732..47727)
                     /locus_tag="PC460_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF2600044.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(47848..49476)
                     /gene="aspD"
                     /locus_tag="PC460_001152"
     CDS             complement(47848..49476)
                     /gene="aspD"
                     /locus_tag="PC460_001152"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF2600045.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
BASE COUNT        19032 a         5635 c         8310 g        16614 t
ORIGIN      
        1 tgaaagtgta agaagttcat aagagaactg cataggtagt agcgaactta tgtgaacgac
       61 aacctcaaaa atgataaaag ttaagaactt acggttctta tatatgttat ttaaaaataa
      121 atataaaaaa ccttcaaaat acttgtttta agtaattgca tattttaata ttatgataag
      181 tgatgaaaaa tggaagttac tcatggtaga ggatatgcat atagtatgtc aaatatagac
      241 atccattatt atggggagac atctttggaa tcctagttat ttcgttgcta ctgtattaga
      301 aaatacagag gagcagatag gaaaatatat taataatcag aaaaaacaag taaagtgagg
      361 tgatagtagt ggaaaaagct tataagttta gaatgtatcc aaataaaaaa cagcaagagt
      421 taattaacaa aacttttgga tgttgtagat ttgtatataa taagtatctt gctaaaagaa
      481 tagaagttta taaaaacgat aaagaaactt ttacttataa acagtgtagt tctgatttga
      541 ccaatttaaa aaaagaatta aagtggctta aagaacctga caaattttca ctacaaaacg
      601 tcttaaaaga tttagataat gcatataaaa aattctttaa agaaaaagca ggatttccta
      661 aatttaaatc aaagaaaatt aatagatttt catataaaac taactttaca aatggaaaca
      721 ttatgtattg tggtcaacat ataaaactac ctaaacttgg tatggttaag ataagggata
      781 aacaagtacc tcaaggtaga atacttaatg ctgctatatc taaagaacca agtggtagat
      841 attatgtatc attatgctgt actgatgtag atattgaagc atttgaaaat actaataatc
      901 agataggttt agatttagga attaaagaat tttgtatctc aagttgtgga gaatttatag
      961 aaaatcctaa gtatcttaag aagtcattaa acaagcttgc taaattacaa agagaattat
     1021 cgagaaaaac aatcggtagt ttaaatagaa ataaggcaag attaaaagtt gcaagacttc
     1081 aagaacatat agctaatcaa agaaatgatt ttctacaaaa actatctact aaattaataa
     1141 aagagaatga tattatttgc atagaagatt tacaagtaaa gaatatgatt agaaatagta
     1201 aactatctcg cttaatttca gatgtatcat ggtcagaatt tattcgtcaa ttagaataca
     1261 aggctaattg gtatggtaga caaattgtaa aggtaggtaa attctttgct agttcacaaa
     1321 tatgtaataa atgtgggtat aaaaacgagg aagttaagga cttaaatata agagaatgga
     1381 tttgtcctag ttgtaatgaa actcacgata gagatataaa tgcaagtata aatatattaa
     1441 aagaaggatt aagactaata acgattcaaa ataaataaca tatgtaagaa ccgtaggaac
     1501 tacggggata gcctgtggag aattagtaag acatatttta gtatgcaaaa ttctatgaag
     1561 caggaaccct gtgactttag tcatgggagg ttcagtttat ttcaaataat attttacgta
     1621 atccatctat gttaattgga ttaatagcag catttggtct tgtactacag aagaagaaag
     1681 catctgatgt tataaagggg tcactacttg cagctttcgg aattataatt atggagactg
     1741 gaacaggtat gttagttagt tcaatagcac caattaatgg agctttccaa agtataagtg
     1801 gtggtgcagt aacaaaaggt ttaagtgatg ttacttttac tgcaagttat ggtggaatta
     1861 taggtttagc aatgtttgcg ggtttagcta ttcacctaat gatagcaaga tttactccta
     1921 ttaagacaat cttcttaaca ggtcacatgc tttattggtt cccattcata tttgtagctt
     1981 caggagttga agctggatta aatggtggag tattaataac ttttgcagca gtattatcag
     2041 cactctattg gtcaataatg ccttggttat taagaaaata tgtgtttgat gttataggag
     2101 atgattcttt tacattaggt catccatctg gttgtttagc acttatatca ggatttgttg
     2161 ctaaattagt aggaaataaa gaaaaatcta cagaagatat aaacatacct caaagtttat
     2221 cattttttag agaaatatct ataagtggtg gaatggcagt gtttttaatg tttatagttg
     2281 ttgggatagt aataccaggt gcgtttaaag atagtaatga gtttattgga acgtatgcaa
     2341 taaagcaagg tctttctttt ggagcaggtc ttataataat gcttcatggg gtaagaatgc
     2401 taataaatca aataatgcca gcatttcaag gtatatctga aaaagttgtg ccaaatgcac
     2461 tacctgcttt tgactgtcca atattattta attataaacc aaatgctgta ttaataggat
     2521 tcttagttgc tatgctaaca tctacagcta tgattttaat atataactac tttaatgtat
     2581 ttaatttgct acttatacca cttgttataa cttcattctt tgagtgtgga gcagcagcag
     2641 taataggaga atcacaaggt ggtatacgtg gagctataat aggtacagct atagctgcaa
     2701 tatttatggt agttttagta ggaatatctg ctgtaattta ctcaggaact attcaaaatt
     2761 ggatactaat atttggaggt aatgaccttt cattatttgg tataatcagt aggtttatag
     2821 gttcactatt tggaggaata ttatagatat gaaatttaaa tacatagatg agataagaag
     2881 attaattgat ataatagaaa acgaagaaaa agtcaatatg gaaaaagcag tacaaatctt
     2941 tgttgaagca atagaaagta aaaatgctat attttcattt ggagcttcac atgcaggaat
     3001 attaacagaa gaattattct atagagcagg tggctgtgtt cttataaatc cagtatttgc
     3061 aaaatctcta atgttagata cagcgcctat aactcacaca agtaagatgg aaagattaga
     3121 aggatatggt acagctctag ctgataaaac tccaattaaa aaaggagatg tagttatagt
     3181 tcattcagta tctggtagaa atccagtaag tatagagttt gctatgaaag ctaaggaaaa
     3241 taaggcaata gtaatatgta taactaattt aagttactca aaaagtgtat cttcaagaca
     3301 ttcttcagga aaaaaacttt atgaagtatg cgatttagtg atagataatc atggtcaaaa
     3361 gggagatgcc tgtgtagaga tagatggatt aaaccaaaaa gttagtccaa cttctactgt
     3421 aatagcaact actgtgatga actctattat agctcaagct actcaggaat tagttaataa
     3481 agggctaaag aatcctccga ttttctatag tgccaatatt gatggtggag atgagttaaa
     3541 taagaagatt tttgatgaat ataaaagtgt aattcattat gaatattaag gttaaaaaaa
     3601 tatgttaatg aactttgaag tttatcaaca aagaatatta ataatatata ataaatgcta
     3661 tcatttaaaa gctgtaggta aaaatctaca gctttttatt attgtagatt aaaaagatgc
     3721 gtaattaaat tttaaattgg agaaaataaa ttggaaggta tcaattataa atattagaaa
     3781 cgttgtcata gaaaaaaact atattaaaaa aaagtacata gaaaaaactg attagacaat
     3841 aaatacatgc tttgataata ttttaaatgg aatgaaaatg tgtagaatta taatgaaaaa
     3901 agaaaataaa aatgaaaaaa gaaaataaaa tggagggaat aaaagtgggt atgttaaaaa
     3961 aattatgttc actagctatg gtagcaatta tgactacaac acttataaca gggtgttcaa
     4021 gtggaaatgg aaaagattca aaaaaggatg gcgaaaaaga aaaactagtt ctttggatgc
     4081 caccagtaga aaataatatg aaggaagttt gggaccctat tttagataaa tttgaggaaa
     4141 aaaataactg tgaagtagat ttacagataa ttccttggga taattattct gaaaaatttg
     4201 caacagctat aagtgctggc gaaggtccag atgtaggata tatgtatgca gaaatgtatc
     4261 cacagtttat agaaagtgga gcagtagaag atttgactaa ctatgcaact aaagaggata
     4321 aagagcaatc tatttatata aaaacatctg aaatgatggg tggaatgtat ggtatgccat
     4381 tccaagcagc aaatccaggg gtattatact ataacaagga tattttagag aaactaggag
     4441 aaaaacctcc taaaacatgg gaagatttta aaagaatttg taaaaaagca acgcaagata
     4501 ctgatggtga tggaaagata gaccaatggg gattagctca gggatgggga gctaaaactt
     4561 ttggtaactt aaactggaac tggtatcctt atttatggca agctggtgga gatatattta
     4621 atgatgattt aaaatcggtt aaatttaatg ataagagtgg tttagaagca gctaatttct
     4681 taaaagaatt acaagcatat gtgcctgaag attctttatc taaagatagt aatgaaatga
     4741 tagaaagcgt atttggacca gggaaagcag catttacaat aatgttatct tctgctgcaa
     4801 caagtgtatt tgatgaatca ttcccggatt taaattgggg atttgtaact ggactagaag
     4861 ataagaaagc agcaactttt ggagcagtag accatttatc tttaatgtca tctgctaaaa
     4921 ataaagaact tgcatacaag ttaatacaac atatgttaag tgttgaatca atgacagaat
     4981 ttcataaagc aataccaaga gcaccaataa caaaaggtga accatatcaa ggtgatgaaa
     5041 gattcaaaga aatggtggaa aatgacaaaa atgtatatag accattagta gtaggacctc
     5101 atggagttga aatatatgaa tatttatgga aagaacttca aactatgatt tcaggagata
     5161 aaactccaaa acaagcgtta gatgatgctg caaaatattc aaatgattta ttagcacaat
     5221 aaaagtttaa agctgtaata tgttgtaaag gactttacag attaaaatta gcattgtatt
     5281 tataaataca atgctaatat ttatattgga gaggataaaa tggaaaactt agttagagag
     5341 tttgaagaaa attcaaaaag aaaaaaaaca aaattaaata ttaaaccata tatgtatata
     5401 ttgccattgg gaataatttt agtggcgttt tatgtgatac caataataat gtcaatatac
     5461 tttagtttta caaagtataa tattattagt ccagctactt ttataggttt agaaaattat
     5521 aaaaaattat ttacagatga aacattaaaa gtatctatta taaatactat taaatttact
     5581 gttgttgtag taccttgcca aactatatta tcattgattt tggctgtttg gattacagga
     5641 aaaggaaata gtaaaatagc ttcatttgct aaaggtgcta tatttatacc agtattatca
     5701 tctatggttt tgataggtat ggtatggagg gctttattga atggagaagg ttctattata
     5761 tatcaggtat taggtgcctt tggaatagaa tcatctaaac ttttaggaga tagtaagaca
     5821 gcattaccta cacttatgtt tatatctatg tggaaaaata tagggtattt tatggtaatt
     5881 tatatttcag ctataatgaa tttaccaaaa cactgctatg aagtagcgaa ggttgatggg
     5941 gctacaaaac ttcaagaatt tataaaaata actgtacctt tattgaaacc aactacaata
     6001 atggttgtat ttttagggtc gatatggtca ttacaagtat ttgacttaat ttatactgtg
     6061 acaggtggag gtccaggtat atctactatg agtatagtta tgcatgcatt taacttaaac
     6121 tttaaaaact ttaattctgg atatgcaatg actgtagcaa acgtattatt tttgttaata
     6181 gcagtagttt cgattttaca aaataagctt gtaagacgag acaattcaga tttttagaaa
     6241 ggtaggtata atatgaaaac agcaagaaga ttattaagtg atggtttgct tttatttata
     6301 gcgtgtgcaa gtttggtacc ttttatatac atgttgataa tatcattgaa gataacatat
     6361 aactcatata gtttggatat atctttttca acagtaaccc ttcaaaacta tatagatatt
     6421 tttactaaaa aaggatttgc acaatatttt tttaatacag caatagtatc tttttcaggt
     6481 gtattattaa atttagtatt tagtacatta gcaggatatt cttttgcgaa gatggatttt
     6541 aaaggtagtg ataagttatt tttatttatg ataatgactt taattatacc ttcccaagtt
     6601 acaatgatac cactatatat aataatgaaa cacttaggat ggataaatag ttatcttgca
     6661 cttattatgc caataccaac tgcatttggc gtatttataa tgagacaagc tattttagga
     6721 gtaccaaaag agttattaga atcagcaaag atagatggtt gttcggattt tagaatttta
     6781 atacaaatag tgttgccttt aattaaacca gcattaatta cactagcaat atttactttt
     6841 atgggagcat ggaacgagtt tatgtggcct cttatagcta caactaaaga tacaatgaga
     6901 acattaacag ttggattatc tactcttaag acattccaga taactaatta tggacagatg
     6961 atggcagggg ctacaataac atttttacct ccatttattt tctatttgat tttacagagt
     7021 aaatttgttg aaggtgtgtc tttgtctggt ataaaaggtt aaatagtcta atttaataat
     7081 atataaagaa acttatataa atatggagga atttaaacta tggaaatttc atatgtactg
     7141 aaagattgca cattcaaaca caataaatac tcagttgtag aagctgaaac ttggaaagat
     7201 aaggatttgg atattgtagt agctaaaggt gaaaaatttg catttcaaat tatgttaaaa
     7261 gcaacagaag aatttaattg tacaattgat aaaagtagtg ctatatcatg gaaaggcctg
     7321 ggaaatagag taaggttagc attaaatatg cctaatagtt tagaaaataa ttttagtata
     7381 aatatattag gatatgttca agatgataca aaggcatttg tgaatgatgc tattttaagg
     7441 gataaagatg tgttagtaga gcaatatttg cctcaaacat tttggattga aggtcaagta
     7501 ccagaagatt ttgaagaaaa taaccttgat ataggtattg atatatttaa aagctttgga
     7561 tatgaagacg aagaaaaagt ttgtacaatt gatgttcctg taaaagtaag aaatgttgtg
     7621 ctaaagcctt tagatgaaag caagttcttt ttagatttat ggcaacatcc aagctgttta
     7681 gctagaatgt ataaagttga gttatggtct gatatacatt ttgaaatagt cgataactat
     7741 ttaaaagaat tagcatcttt aggagaaaaa gttgcaacag ttatagtttc agattatcca
     7801 tgggcagggc aaagttgtta taaagtttat aaaaatccat caaacttata tgaatataac
     7861 atggtaagtg tatcaaaagg attggatgga aagattaaat gtaactttga gagtatggat
     7921 aggtatataa gcatagctga taagtataaa atggctaagg agatagattt atttggtcta
     7981 ttaggtaatt ggtgtgctgg tgagtttgga aatcctgtag aaggttataa ggaccctata
     8041 agagtaagat attttgatga aaaggataaa gtatttaaat ttataaacaa tacaaatgat
     8101 ttaaaagaat atataggatt agtattaaat catttaatag agtgtggatt atgggacaga
     8161 gtaaggatta tagctgatga accaaataat ccagaagtag tcaaagagtg tatagaattt
     8221 ataaattcta ctgtaagcac tcatcaagtt aaatataaat cagcaactca cgaccaaaac
     8281 tttttagaca gagctaaaga tgaaattgat gatatgtcaa taaatttaaa acttactata
     8341 caaaattata aagatataga aagtcttaag aaaaaaatca atgacaaggg tgggatattg
     8401 acttggtttg tatgctgttt tccagagaaa ccaaatagtt ttttaagttc tccttttgta
     8461 gaaaatagaa taataggatg gtatacttat tactttggtc ttgatggatt tttaagatgg
     8521 gactataatt tatggacaga agacccttgg aaagattcaa gttacaaatt ccctatttgg
     8581 aaagctggag atatgttctt tgtttatcct ggaaaagatt taaagccagt tagaagtgtc
     8641 agaatggaga atttaagatt tggaatacag gactttgagt tatttacaat gttagaaaaa
     8701 gaaaagggaa gagagtatat agaagttgag cttatgcaag aattattaaa taaaaaagaa
     8761 aatgctgaaa ttaaaggttt tggtgacata gaactaggat attcacttga taattgggga
     8821 tatgataaag ttaaaaaata tgtattagag ttgttagaca gagtataaaa aatataagag
     8881 tgtctcaaaa tgaacttttt agttcatgag gcactctttt tgtatgaaat caatatagtt
     8941 tcattgtttc aacaatgaaa aataggatta tttctattct aagacagccc tttagttttt
     9001 tagttgtatt caatgtttta aaactaacta taaacgctat aaagtttata aatgacaata
     9061 attatatata acatgatgta ctttaatttg ctataatagg gattgaataa tattatatgg
     9121 aagataacat tgtttggggg gattaatata aaaagaatag gtattattat aatgtttttt
     9181 ttaagtatat cttttattgg attttatctt gtaataagag ataacagtga tacagttaag
     9241 atgcaacgag ggataaatat aggtaatgca ctagaatcac ctaaggattt tccgtgggat
     9301 gtaaaaatgt caaataagtt ttttgatgat ataaaagatg caggttttga cacagtacgt
     9361 ataccagtaa ggttttctga ttatacgtca gattctgata attttaaaat tgatgaagaa
     9421 ttttttaaaa agattgataa atatgtagat tatgcattgg ataaagactt aatagttgtg
     9481 ttggatttac accattttga agaaattatg aaagaaccaa gagttcataa agaaaagttt
     9541 ttaaaaatat ggcaacagat agctaatagg tatcaaaaat atgataaaaa attggtattt
     9601 gaattattaa atgaacctaa agaaaattta tattcacagt tactaaacga atacattgaa
     9661 gaagcaataa agataattag aaaaactaac cctaaaagaa ctattatagt aggaccatat
     9721 aatttctatc aaattgatta tttgaatgaa ttaaatattc ctaaagattc taatatagta
     9781 gtttcctttc actattacga gccaaatgat tttgcatttc aaggaaatat atatcataag
     9841 ggatttgagc acttaagcaa tattacttgg gaaggaacaa acgaacaaat ggattattta
     9901 aaaaaaaggt ttgatactgt ggaaaattgg gcaaataaaa ataaggtaaa aatcttcttg
     9961 ggtgaatttg gaataacaaa agaggcacca gaagcttcaa gaagagcatg gataaaagct
    10021 gtaagagaag aagctgaaaa acgaaacttt tcatgggctt attgggagct tgcatctgga
    10081 tttggtatat acaatcaaat tgaagggact tgggatagag atatattaag tgcattaata
    10141 gaaaagaggt gattttatga agaaaaattc aaaaaagata cttataactt taactattat
    10201 tactaatgtc atatatatat tgtggagaat tttttacaca gtacctaaag aagaagggaa
    10261 gtttgcctta atatgtgcca taatacttct ttttgtagag ataatgggca tgatggaaat
    10321 gtttgtgcat tattatggta tgtcaaatat tgagtaccct gaaaaaccaa tcataagtga
    10381 agaactttat ccacatgtag atgtattcat tgcaacatat aatgaatctg ttgatttggt
    10441 gagaaaaact gtaaatgggt gtattcatat gcagtatcca gataagaaaa aggtacatat
    10501 atacatatgt gatgatggaa atcgtgaaga aatgtgtatt ctagcagaaa aaatgggtgt
    10561 aaattacata actagaactg aaagagaagg agcaaaagct ggaaatttaa ataatgcaat
    10621 gcaacataca aattcccctt tgattgctac atttgatgca gatatgatac caatgcatga
    10681 ctttttaatt gctacagtac catacttttt gaaaaatgag caagctaaaa aagatggaga
    10741 aaaagaggag tatgaaaagg ttggttttgt gcaaactcca cagagttttt ataatccaga
    10801 cttgtttcaa tttaatctgc actctgaagg aaggattcca aatgagcaag attattttta
    10861 cagagacatt caattggcta gaaataggac aaattctgta atttatggtg gtagtaatac
    10921 agttatttca agggaagcac ttgaagaagt tgatggattt tatacttatt ctattacaga
    10981 agattttgca acaggtatct taattcaaag taaggggtat agatgttatg ctattccaga
    11041 agtacatgca tcaggtttat ctcctacaga tttaaaaagt cttattaagc aacgtgaaag
    11101 atgggcacgt ggatgtatac aaactggtag acgtctaaat attttgttta ggagaggttt
    11161 aggtttttgg cagaaaataa gttatatttc ttcaattact tattggtatg caagtataaa
    11221 acgctttgta tatataatgg cacctatttt attttctgtg tttaatgtga ttgtagtaaa
    11281 atgtacgtta cttcaagtat tggtattttg gttgccaatg tatattttaa gtagtttatc
    11341 tcttaaaata ttttctcaaa atataagaaa tactagatgg accaatatat atgaaactat
    11401 aatgtttcag tctttaatgc cagcagtaat acttgaaacg tttgcaatat ctaaaaataa
    11461 attttcagta acaaataaga gtaaattaga agaaaataga atgtataagt ttttacaggg
    11521 aataccttat tttatttaca tggtcttatc tatcatagga attttaaaaa tgtttgtagc
    11581 aatatttaaa atgagttcaa tgacatattc agttgtatta ttttggttga ttggaaacct
    11641 ttttaacctt gttatggcta cattatttat atcagggaga cagcagttaa gaaagtctga
    11701 gcgttatatt gctgaaattg attttaagtt aaagcaaaat tcttatgttc tttcttcaaa
    11761 aacaattgat atatcagaaa atgggtttgc atttttgcta gaaaatccag aatatatttc
    11821 cccagaagaa gagtttgagg tagagtttag agaaaaatct ggaaatgaaa tgtatatagc
    11881 taatatgaaa gcaaagattg ttaatgttgt ggaagtaaat tcaaagtgga aatatgcagc
    11941 ttatattaca catattgaag atagtgaaat agataattgg atgtgtattg ttcacgatag
    12001 aatacctact ttaccaatga caatatctaa tcaactaggt ttctttgatg atttgcaaat
    12061 taatgtaaaa aaacgtattg aaaaaactag aacattatca cgaagaagtc caagaatcaa
    12121 tatgaatttt cagatggata taaaaaatac aggtaaatta agaatagtga attttaatta
    12181 tcaatatgta ttattaaatt ttgagaataa aagtatttat cctaaagaaa tagcattgga
    12241 aattaatgaa aatattgtgt tagaatgtga tttgtgtgag ggtaaaattg atgaaagagg
    12301 tatcttatat aaagtaaata acattgacag cataatgcaa aatttctttt taagagatga
    12361 aatgatggat tggatattac aaaataagaa aattcttgat tcaaagccta gtgaaaagaa
    12421 ggaaaaaagt atagatgagt ttgaacccat ggaatatatt taggagagat gaaatgaaga
    12481 ctttaaaagt gtttttttgt tctacaaaat atcttatttt ggtgtttatt ttttttataa
    12541 atactacttt tataagttca gcaacaagtg ataaaatttt agatttatcc tttaaaaaga
    12601 tagagactga cttaagttct aagattacat atgaggatac aggtgtaaaa atagaaacgg
    12661 atagttctaa aagcgataag gaacgttatt tatatattta ccaaaatata aaagaaaatt
    12721 ggagcatgta taataatttt tatattgaaa ttcaaaataa aaataaaagt tctcagagaa
    12781 taaatttaag catacaaagt aaaaatatgc ttgaatttag attaaaagaa ggtagtgaag
    12841 tatttttaga aggtaaaaat ataatatatt cagataaaat aaaagaagga tgcatagaag
    12901 taccaggtga atttgaaggc aaaatttatg tgaactttaa tagccttata aatgaggaaa
    12961 gtaatgttgt gctggactca aatatgcttt caaacattgt tagttgggga attacgttta
    13021 ttccatctga tgaagaacat aatattgtta ttataaaaaa gatatcttta ttgtctgaag
    13081 agaaattaag atttttaaat aatattaaaa taattggcga tgaagaagta caaataccag
    13141 tattgggtca aagtataagt cagtatgagg tattaggtct taaaagtgat tctaaaatta
    13201 agtactctct tatgggaaaa caagataatg tgagtatatc tcaaaacggt aaattaacat
    13261 taagtaataa agccaaacct ggacaaatta ttttacaagt taatgttgat gataagttta
    13321 aaattggaaa gaaaataaca ttaactgaat cttggagtat taataaaaaa gataaggatg
    13381 gtgttccata tacattagta tctcctgagc aatcaccaac agtacaagat atgaaaaaaa
    13441 taaattttat gaataatatc ataacgtttg ttcgaatttt atttgtatct ctagtaataa
    13501 tatgctttgg aatatattta tattggaaaa aatgttcaaa aactaagtaa agagaggagg
    13561 ttagattgtg ttattttcaa gtttaatttt tttattttat tttttaccta taactttggt
    13621 tttatattat gtatttaggt ttaaccgaac gatacagaac atgatattgc ttgctgttag
    13681 tttatttttt tatgcttggg gagagccaaa atttgttgtt atcatgatag tttccataat
    13741 aatgaactat atatttggtt tactggtaga tagatatagg gaaagtaaaa taaaagtaaa
    13801 agttttttta gttttgatgt gtgcatacaa tataggtgtt ttatttatat ttaaatatct
    13861 agcttttgcg ctaagaaata ttagtgaact tattagtact gaattgacta tacctaatat
    13921 tattttacct ataggaattt cattttttac atttcaagga atgtcttatg ttatagatgt
    13981 atatcgtaga catggagaag tccaaaagaa tcctttttat gtgggactgt atatagcatt
    14041 tttcccacaa ttaatagcag gtcctatagt tagatatgag agtgtggcag aacaaattct
    14101 caatagaaaa gaaacttgga ataaattttc tattggtact tgcaggttca taacaggtct
    14161 aggtaaaaaa gtattaattt caaataacat ggctatagta gcagactata tttacactat
    14221 gaatagtcaa ggagaaatag ctgcatcact tgcatggctt ggttcaatag cctatactct
    14281 gcaaatattc tttgattttt ctgcttattc agatatggct ataggcttag gtcttatgtt
    14341 tggttttaaa tttgaagaga actttaatta tccatatata tcaaaatcaa ttagtgagtt
    14401 ttggagaaga tggcacattt ctttgggaat gtggtttaag agctatatat actttccgct
    14461 tggaggttct agagtagcaa ataaagatat tatgattaga aatatgttta tagtgtggtt
    14521 attcactgga atatggcatg gagcagaatg gacatttgta atttggggga ttttaaactt
    14581 tgttttccta attatagagc gatttataat gtttgaaaag attgaaaatc acaattttat
    14641 aaaacatatt tatgctttat tagtagttaa ttttggatgg gtattgttta ggtcacctaa
    14701 tttaaaagaa gcatataatt attttaaagc tatgtttgga ggcaatggag ctatatggag
    14761 tgattataca tatatgtttt taaaagaata ctgggttttc tttatatttg catttatttt
    14821 tagtgtacct attgcaaaga aaatcaataa gtttgttgtt gaacaagcta aatatagtat
    14881 gatatttaac gtattttatc caatctcagc tattttattg ttttttattt ctgtgacata
    14941 tttagtaact ggaagctata atccatttat ttattttaat ttttagttgg gaggaaaagt
    15001 atgaatagat tttttaaagt aagattaata actgcaatta tgttcttaat tattttattc
    15061 gctttctcta tctttaatgt ttgttattct ggaaaagata cgatacagga aataaaaaat
    15121 tcaatagctg aaaaaagtag tttaaaagat acaatatcat ctattgataa tacagtgaat
    15181 gacaaagtaa tatacaaaaa tacgttcatt gaaacttatg gctatttaca aaaattaatg
    15241 ggtaaaaatg agttttcaaa atttagtgtt gtaaaagata cttctggaaa gatgcattat
    15301 acatattttg ctaaagctcc aaatccagtt ggtgtgatag cagatagggt aagaaaattt
    15361 tctgatgaaa tggaaaagca aaacactaaa ttggtttacc taatgactcc agataagtat
    15421 ataaaaggag tgacaaaatt tccaaaagga ataccttatt cttatagtaa tgagacagca
    15481 gataattttt taaatcaact aaaaaagtat aatgtggact atattgattt tagagaaaat
    15541 attttaaaga gtggtattcc aaaagatgat ttatttttta aaacagacca tcactggaaa
    15601 gtagaaactt ctttttgggc atttggagaa ttagttgagc aacttaataa aaaatacaat
    15661 atgaatttag atgaaaatca ttattataga gataaggaca attataattc aattgtatat
    15721 cctaagtcat ttcttggttc tatgggaaga aagactggta tcttatatgg tggtatagat
    15781 gattttacat taatttatcc taaatttaag actaattata cttattatac agattctaaa
    15841 tctcaaaaat ttgaattaaa aggaaggttt gaagagagtt taatattatc atatcctttt
    15901 aatgcagacc ttgatttaat ggatggtcaa tcagataagt attttacata tttattgggg
    15961 aacagaccat tagttaaaat aaaaaatata gaaaatccag atggattaaa agtgctgttt
    16021 gttaaagatt ctctaattgt accaacagca gcattttttt cctctgtttg ttcatctata
    16081 gatatgatag accctagata ttatgatgga gatatactag aatatgcaaa atcccatgac
    16141 tatgactttg tttttatatc tgtatatccc caaaatctta caaaagaatt ttttccgttt
    16201 tgtgagtaaa tttctaaaaa agttttctga taaaataaat ggctaactgg taaaaagtta
    16261 gccatttatt ttgagtgtta ttctaagaaa tatttttttt actaaatact tctttatcta
    16321 tttttccaag tgattttcct acagtaattg atgataccat agcaccatta acatttatca
    16381 tagtacgtcc catatctagt atagggtcta ttgcaagtat accaccaact aatggaaaat
    16441 aagaaccaag accaactcca gagataacaa ctgaaacggc cattgtagca gttcctggaa
    16501 gaccagctat tccaaacgaa ctaattgtta taacaattaa caacataaca taaaagctca
    16561 tatccatgtt tactccagcc atattggcaa gtgttacagc cattaaagca ggatatatac
    16621 ctgcacaacc attcataccc atatttgcac ctaaacttgc tgtaaagctt gatacaccct
    16681 catcaactcc gtgattttgt actagacttt ctatcgttac aggcaatgtt cctaaactag
    16741 aacgtgaagt aaatgccaga agtaaagctt cagttgaatt ttttatataa gtaatgggat
    16801 ttagaccatt taaagttata aaaattaaat gtataataaa taatattgca actgatatat
    16861 aaagagctaa tataaactgt aaaacagata ctattgaact aataccttgt gaagttattg
    16921 tatttgcaag cagagcaacc acagcataag gcataaattt aataacagtc atagacacac
    16981 ttacaattat tttgtaagat gcttctataa aatctataaa tggttttatt gtctcaaaat
    17041 gttttttact taatctcttt attgaggtag ctataaatgt agcaaatatt attgtagcta
    17101 caatatttcc atttgccata gccattacag gattagatgg tagtaatcct cttaaagtat
    17161 ccacaagaga gtttatttct ttaagttctt gattttcaac taaagtaggc atattagctc
    17221 ctacacctaa cttcattata tttgcaacga taattccaac aattgcagct atagcagtag
    17281 tagcaagaag catacctaaa gatttaaatg tcagagttcc aagattgtta tctttcatat
    17341 ttataattac ttttataatg gataagaaaa ctaaaggtac tactaacatt tttaataagt
    17401 ccataaatcc agaaccaaat aggctatacc acttacttac ttcttgtagc caagtgactt
    17461 gagttgtatc attaggaaaa ccagcaaaag cttgtattat gattcccaat atgagtccta
    17521 gtactgtagc atatattgtt ctcaatgaaa attttatttt tttcttttca aattgtctaa
    17581 caataaagaa cgtacctaga agtaatatta tgaaaaaaat tgttttaaaa tctgtaatca
    17641 taagaaattg tgataaaaat gaactttcca atatttttct ccttttattt aaaaataaat
    17701 ttttctaagt ttaaatgata tcaatatatt aaaaaaatgt caatattata tcacaattta
    17761 cttaaataga gtaaaacaag ttgattttga aaaatgtgat taagtacaga aaaatagggt
    17821 ataataaatg tttaaagaat ttgaattgtg gtaagaaaat aaaaaatagc ttttataaga
    17881 tttgatttca aattaaaata tgttaaataa ataactaaaa attattataa aaaatgtttt
    17941 catagaatat atatcaaatg aaaagcttaa aataagtgaa gaaattaact agacatcaaa
    18001 ggagagataa aatgagagta ataataattg gtggtgttgc agctggtatg agtgctgcag
    18061 caaaattaaa aagaataaaa cctgagtatg aagttgttgt ttatgaaaaa actgaaatag
    18121 tatcttttgg tgcttgtgga ttaccatact ttgttggtgg attttttaat gatgctgatg
    18181 aattattagc aagaacacca gagaaactta gagaagctgg aattgattta aatatattta
    18241 gagaagtcgt tgaggttgat agtgaatcta agaaaataaa agtaaaaaac ataaaaacag
    18301 atgaaatata tgaagattac tatgataaat tgatgatagc aactggagca agaagtatta
    18361 tgcctcctat aaaaaacata aaattgaaga atgtttctac attaaaaagt ttatatgatg
    18421 gagaatatct aaaaaaactt ttaagtaatg aagataataa aagagtgaca ataataggag
    18481 ctggttttat agggttagaa gcagttgagg catgtaagaa attaggtaaa gatgtagatg
    18541 taattcagtt agaagataga atattgccac aagtatttga taaggaaatg acggatgtat
    18601 tagaagaaga aataaataga cataatgtta atttgcactt agatgaaatg gttgtagaac
    18661 tttgtggaga agataaagta gaaaaagtta taacaaacaa aggggaaata gatacagatg
    18721 tagtaataat tgctactggc gtgagaccta ataccgagtt tttgtcaaat actaatataa
    18781 aaatgctaaa aaatggagct atagtagttg atgagtatgg aagaacttct gtagaagata
    18841 tatactctgc tggagattgt gcaactataa agaatatagt aagtaatgaa aatgtatatg
    18901 ttcctttagc tactggagct aataaattgg gtaggatagt aggagaaaac ctagcaggaa
    18961 gagaagtatc ttatcaaggt tcactatctt caagctgtat aaaaattatg gatatggagg
    19021 cagcatcaac tggaattaca gaaaagcaag ctaaagattt aggcataaat gtcaaatcta
    19081 aatttatttc tgattataat caaactcatt attatccagg aagaaataaa atatatgtaa
    19141 agcttattta tgatgcagat acaaaagtta ttttaggcgg acaagtggct gggtttaagg
    19201 atgctgtcca aagagcaaat gtattggcag ctgctatctt tggaaaaatg accacaaatc
    19261 aacttggaat gttagattta tgttatgctc caccatttgc aagaacatgg gatgttttaa
    19321 atgtagctgg aaatgtatct aaataatatt tagattatag ctaatatagt tgacaagcaa
    19381 ttaagggaat gaatttacct aaactatttt tatcatggta aaattcattc ctaaattttt
    19441 attccaaatt tttattccaa atttttagaa ataaggattc cattaaatta ttcatcaaaa
    19501 tttaaacgca agcttaatct atcttctcca aggatagtat tttcaaaaac attagtagca
    19561 ttttctaaat acatactagg tatgtctaaa gaaggactaa aatgagtcag taaaagttgt
    19621 ttgacatttc caagttttgc aagattagca gcttctctaa aagtcatatg tttgtttcta
    19681 acagcttttg atatatctaa atcatcacca tacatggctt cacatataaa taaatcacta
    19741 tcttttatga aatctggtat tgattctata ggtcttgtat cggttgtaag acttatctta
    19801 actccctttc tcaattctcc taagaccata tctggagtgt attgttttga atctaaaact
    19861 atattttgac cttcttggag tttgttccat aatattttag gaacttcgtt tttagttgcc
    19921 ttatctatat caaatttagg gtttcttcta aaattaaaac tgtatcctaa acactctata
    19981 gagtgttcta aactgattgt tgaaatttct aaatttctta aaactttatt atctaaagaa
    20041 aaattgcctt gagggttttc tataattttt aaagtgtatg gtacatattc tactaaattt
    20101 ctcatagaac gtatacaatc gactattcca actggaccaa ctatagttaa atcacttgta
    20161 cgaccactat ttccaattgt tgataaaagt cctaaaagtc caaatatatg gtcaccatgt
    20221 aaatgggtta tacaaatcaa gtctatatct ttaaagccac atttttttag cttcatagat
    20281 acctgagtac cttcaccaca gtcaattagt atttttctac ctttatagtt tataaataca
    20341 gaggatagaa atctgtttgg cataggtaca tttccaccac atccaagtaa agttaaatct
    20401 atcatttttt cccctttcta aaattaacac aaatctgttg tttgttttta ttatgatatc
    20461 ctattataat tattttaaca ttaaatataa caataaatgt gtatttgagt caagaaatat
    20521 tgtcaaataa agtttttata tagaaatgta taaaaatttg gaaaacaaaa agtgaaaatt
    20581 atatttagga gcatttaaag gaggaaaatt taatgaaagt attaaagaaa attactattt
    20641 tgtcattaat tacattaacc ttagttggtt gtagtaacgg agaagataaa aaagtggttg
    20701 aaggttctaa gaaaattcaa aattctaaaa aagatgaaca gcctataata gtacataaag
    20761 atgcaaatat attgaaagat aggtatgagc caccaaaagg atttaaaaga gttgatgtag
    20821 aaaaaggtag ttttgaatca tttcttagga atactaagct aaaaaattat ggagagaaag
    20881 taaagtatta tgatggaaga actaaggaat caaaaggtat ttatgatagt gtatttgatg
    20941 ttgatattgg caatagagat ttgcatcaat gtgcagatgc aattatgctt atgagagcag
    21001 aatacttgta tcagaataaa ctatatgatg aaataagttt tgattttgtt gatggtttta
    21061 aggcgaagta ttcaaaatgg gcacaaggat atagaatttc agtaaaagat agtggttcaa
    21121 gctggtataa agcaactgaa tattcaacat cttatgagag ttttagaaag tttatggata
    21181 tagtgtttgc atattcagga acgttatctc ttgaaaaaga attggagcct gtaaaagttg
    21241 aggatatgaa aataggagat gtgtttatag ttggtggaag tccaggacat gcaagtataa
    21301 ttatggatat ggcagaaaat gaaaagactg gtgaaaaggt atttatgata gcccaaagct
    21361 atatgcctgc acaacaaacg caacttctta ttaatcgtaa aaatccaaat ttgagtcctt
    21421 ggtatagttt agattttgaa ggtgatttga caatacctca atggactttt aaaagagacc
    21481 agttgaaaag attttaaaat aataatttta tataataaaa gaaaaatata gatgtgaaaa
    21541 aatgtaatta gattaaaact aattggatat ggacaaaatt aaattgatag ataaatatta
    21601 aaaattacac taattaatct tactatagtt taatatttat ctaaaaagtt ttatgtaaaa
    21661 tataaatttt atattaaagc taaaactaat ttgaggaggc taatttatat tgaagattag
    21721 aaggtattta attttggtaa ttataattct aattacaaca agtcagagtt ttatttctta
    21781 tgggctagag cgatttgaag aaataaccat ttttcacact aatgatatac atggaagata
    21841 tgctgaaggt gatgaccata tacaaatagg aaatctagcc acattgaaaa aagaaactcc
    21901 aaactcaatt ttagtagatg caggagattg tttgcatgga cttccaatag taaatatgga
    21961 tagagggaaa agtgcaattg aattaattaa atgtgctgga tatgactata ttactcctgg
    22021 aaatcatgac tttaattatg gaaaagatag actgtttgag ttatctaaaa gtgctaattt
    22081 aggagagaat actttaaaat ttttatcatc aaatattttt gaaaatggaa aaaaagtttt
    22141 tgatagtaat gatataaaag aaatagatgg aataaaaata ggattttttg gactctcaac
    22201 ccaggaaaca aagagcaaaa ctggatttaa aaatgtggaa ggattagagt ttaaaaatcc
    22261 tatagaaagt gcaacagaac aagtaaatga attaaaagga aagggtgcca atgtaatagt
    22321 tgcaatatgt catattggta caaatagttt aagtaagcca aatagtatag atatagcaaa
    22381 tgaggttcaa ggaattgatt tgattattga tggacacagc cacactaagt ttgaaaatgg
    22441 taaacaagtg ggtgatacat tgattgtaag tgcaggacaa tatttagaaa atattggcga
    22501 gataaaacta actttggata ctaatgatgt agaaaatata aaaatagaaa ataaatcagt
    22561 tagattaatt ggaaaagaag aagcattaaa gtataagctt gactccgctg taagtaaaaa
    22621 ggttgatgaa ataaaagaac aacaagaaaa aatattagat aaagttatag gggtaactac
    22681 aaatactcta gatggttcat atgaaaatgt aaggacaaaa gaaactaatt tgggtaattt
    22741 aataagtgac attttactag ataaaactaa ggctgatatt tctttattta atggagggaa
    22801 tattcgagat acgatagaaa aaggcaatat aacaagaaga gatattgtag atgtatttcc
    22861 ttttagcaat actatagtta ccaaagaact tacaggagca cagattaaag atgtattgga
    22921 gcatggagta aaactttatc cagaaaaaag ttcagccttt ttgcaagtgg gaggaatttc
    22981 atattacttt aatcctaaac aaaaagaagg cgaaaaaata acaaatattc aaaaagaagg
    23041 aaagccatta gatttaaata aaaagtatgt agttgcaacg aatgattata tagcatcagg
    23101 tggagatgaa tttccttgtt tttcaaaaga gccagtacta aaggaatttg gaaatttaga
    23161 gagtgcagtc attgaatata tagagtacaa aaaagaaata tcaaaaaatg tagatggaag
    23221 aattggtatt aaatctaaag atgagttaat aagcaagaat catgagagtg atttgatatt
    23281 aagtgaaaat gaaaaaaata tcaatgataa tgaagagaat ctatctaata atataaaaag
    23341 ctctgatagt gataaaaata aaagcttaga taaaacaagt gctagagaaa gttctaatct
    23401 aggaatatct aaaagagaag ctaaagaaaa atctcctaaa acaggagatt taggtttttc
    23461 aaatagtata attatattta tagtttcaag tacattgata tgtttattga attttaatca
    23521 aaaagagtta aaggataaaa agtcaaaata aaaattaatg agcaaaaatt agtagatagt
    23581 aatttaattt tagataatag taaacagcaa aggaaattag ttatataaat actaatttcc
    23641 tttgttttat gttttaacat gattactttt aaataaaatt gtattttatg aaaaataatt
    23701 acactaaatg gcatagtatt atggggattt ttagttttta ttcaagtaaa tgaaatcata
    23761 ttaacaactt ctgatatatt tacaaattga taaaaaaatg attttattag tatgatataa
    23821 tttagctagt atatatgatg atggaggata tagggatgaa taatatatta cttttggaag
    23881 atgacaaatc tttaaataga ggaattagtt ttaaattaaa aaaagaagga tacaatgttt
    23941 ttagtgcatt tggtatagaa gaagcaaaaa atattttcgc aaaagaagag atatgcttaa
    24001 ttatttccga tataggtcta cctgatggta gtggttttga cttttgtgag gagattagaa
    24061 agaaaagtga tgtttacata ataatgttga cagctcttga tgaagaggtg gatattgtca
    24121 caggatatga tttaggtgca gatgattata tcacaaagcc ttttagcttg atggtattaa
    24181 tatcaaaagt gaatgcgctt atgaaaagag taaatacagt aaaaaattat actctattag
    24241 tttgtgatga tttattcttt tattatatag aaaataaatt aattataaga acagatgata
    24301 aggaagaaga gataatttta agtaagacag agacaaaact attaaaatat ttgatggaaa
    24361 attctatgca gacattgact aaagagcaac tgttagaatc tttatgggat agtagtggta
    24421 attttgtaga tgataatacg attgcagtaa atataagaag acttagacaa aaggttgaaa
    24481 aaaatccatc tgaacctaaa tatataaaaa cagtaagagg ggttggatat atgtggggag
    24541 aaaggagtat aaaaaaatgc taaaaaatat gaacctcaaa gatggtttaa gtgtgaaaga
    24601 tgattatata atgagaagag ttattgtata ttctgtttta tctgtactaa tagtaaccct
    24661 tgtatttata ctttttgaat atagcagatt acataaattg tatgaacatc aaataaaaaa
    24721 cactcaaaat attgctggaa tattgattga aaaatatcct aatgatgaaa tagatatagt
    24781 aaattctatt tataattctg attatagtaa ggtgtctact ggagaaaagg catttaagaa
    24841 atttggctat ggattggaaa ataaaatgtc aaatgataaa aattttagct tatacttaaa
    24901 atcttttttt aaggaatcat tttttatttt tttaatgatg tttatagtag ttataggtat
    24961 aatatattat tttataagat acataaataa aagattgagt aaaatttatt ttatagtaga
    25021 taagatgtca caagaaaact acttgaagga cactactgag tcagaagaaa ctggttatct
    25081 agtagaatat aatgaatact ttaaagaagg catatttagt aagataaata actgtctata
    25141 tgagcttaat agaagtttaa agataaagtt tattaaatta gaaaaagaaa aagaaagtgt
    25201 aaaatcatta gtaacagata tttcccatca attaaaaact cctttagcat ccttaaaatt
    25261 atacaataca ttacttatag aagaagaatt agaagaagat gaagccaacg agttcttatt
    25321 aaccaataag gcttctataa ataaattaga aaatttaata aattctttag tgaacatatc
    25381 aaggttagaa attagcatga taaacataaa aaaagaagat aatgatataa aaaatactat
    25441 tctaaatgca gtaaaaagtg ttacccctaa ggctaagagc aaaagtataa cattaaattt
    25501 aaatgaattt aacagtagaa ttataccaca tgataaaaag tggactgagg agagtatttt
    25561 taatgtatta gacaatgctg taaagtatac acaggtaaat ggagaaataa atatatctgt
    25621 agaagaaact gctaatttct ttaaaattat aatagaggat aatggcatag ggattgaaaa
    25681 aaatgagttt aataatatat ttaaacgttt ttatagaggt tctgctgatg aaattgaatc
    25741 tgtagaaggt agtggggtag gattgtattt aagtagaaag attttagaag aacaaggtgg
    25801 aaatattata gtttcttcaa aagtaggaat agggagcaag ttttctttgt ttcttacaac
    25861 tatgtaatat taaatgaaag gaaaatgtaa gcctctacat ttattatata agtataaaca
    25921 gctccagaaa taaaaaatta ataaaatatg gagtaatata acttagaatg agaggataat
    25981 tttatgaaaa tactatatac tgaaaattta tcaaagcatt atggaaaagg agaaagctta
    26041 gtaagagctt tggacaatgt tgacttagaa ataaatgaag gtgaatttgt tgcaataata
    26101 ggaaaatcag gttctggaaa aagtacactt ttgcatatga ttggaggtct tgatatacct
    26161 acatctggga aggtgtatat agataataaa aatatattta cattaaaaga agaagaactt
    26221 gctgtattta gaagaagaaa aatagggttt atatttcaaa gctataattt aattccatct
    26281 cttaatgtat gggaaaatgt agtgttgcca atcggtttag atggtagaga agtagatgaa
    26341 tcttttataa aagaactttt aaaatcatta ggacttgaaa ataaacacga cgttttacca
    26401 aatacattat caggtggtca acaacaaagg gtggcaatag caagagcatt agctacaaga
    26461 ccagctataa tacttgctga tgaaccaact ggaaacctag attctaaaac gtctgatgag
    26521 gttatgagta tattaaaatc tatgtctaag aagtacagtc aaactctagt tatgataact
    26581 catgatgaca gtatagccca aatggctgat agagttatat tcatagaaga tggcagggtg
    26641 tcaaaggttg gtgataaaaa tgattaatac tactaaaata gcaaaaagta atttaaagca
    26701 aaataaatca aaaagtattc taataataat tactataata ctttcaacct taatgctttc
    26761 ttctattgga atttatattg ttgatgcagg agcatatcaa aaagaaaata ctataaagta
    26821 ttcaggcaat tatcaaggta tattagcaaa tgtagatgaa aaacaggctg atatattgag
    26881 taatcatgca gatgttgaat taactggtga aatgaatgga gtaggtgttg aaaaactgga
    26941 agatgattca aatatttctc tagcatacat gaatgaagat gctttgaaat taaatagttt
    27001 tgaatttatc aaaggaaaat tgccatcaaa agagaatgaa attgtgttag atagtggagc
    27061 tttaaaggca cttggttata aaaataaaga tttaggtgaa aagataaaaa tatcttatga
    27121 tgactataaa aatgataaaa aaattgaaaa agagttcatt ataagtggaa tcttaaaaac
    27181 tagcgaaata tctgaagcag gtaaatatta ttatgctatt atatcagaat catatatgag
    27241 aaatactaga gatatgtcac aagaagattt taatatttat gtgaaattta atgataaaag
    27301 taatttatca atagagcaag caaaagaaaa attagataaa attgctaatg atataggact
    27361 ggatactatt aatactgctg taaatgaaaa ttatataaat gctttaaaac cagatatgga
    27421 aacgattatg ggtggagttt ttgtagggtt agtaattgta ttatctagta ttttagtaat
    27481 atacaatata ttttatattt caatagttac aaaggttcaa gaatttggaa aacttagagc
    27541 aattggtgca actaagaaac aaataaaaaa tatagtgttt aaagaagggt tcatattagc
    27601 cggaatagct ataccaatag gaataatttt aggctatgta cttgcaaata ttataataaa
    27661 atcatttatg gatatagatg taaaatcatc acgattacca gttatattat cggttgtggt
    27721 aataagcttt atatctgttg ttttatcact tttaaaacca atgaaagtag catcaaaagt
    27781 atccattgtt gacgcagtaa gatatacagg aaataaaata agtaataaga gtaagagaaa
    27841 aggatataaa aatataaatc taaagagatt atcacatgcc aatttagaaa gaaataaaaa
    27901 acgtacttac atgacattgg catcacttat acttagtgga actatattta taacagtatc
    27961 aactgcctta gaaagttttg atgctgagaa aatggcaaga gaacactttc cttatgatat
    28021 agaagttaga ctatctggtt atgaaatgaa cagtgataaa aatcctaaag ataatttgaa
    28081 tattttacaa atggataatc ctctaagtaa agattttttt aatcaaataa agaacatgga
    28141 aggaattaaa agaatagaat ctgcaagaag tgtaaaaatt ggtatggagg actatgatgt
    28201 tgaatttaag tatgaccttt tacaaagtat aaatgaaaat gatgtaaaat cattaaacaa
    28261 gaatataata gatggaaaaa taaatcttga aagacttcaa actggagatg aaatagtaat
    28321 tactcatgtt gatacagcaa aagaaatggg agtaaaagca ggagataaaa taagattaac
    28381 tttatatgat ggagataaaa agataaaaaa agaatttaaa gttcaagcaa tagctatggg
    28441 ggttccaagt tttggtatag ggaaagattt tatagataga actttgaaat acgattcaac
    28501 tagtagcttg ggaatatata cagaagaagg aaagtatcaa gaaatcaaag atagtatcaa
    28561 gaagattgct aaaaataatg gatttttaga gactgatttt atagatagta gaattgagtc
    28621 aaataaggct actatttcat ttataaagat aatgggatac acgctaacag gcataatagg
    28681 tgtgattgga tttatgaatc ttgtcaacac aatgataact agcatagtta ctagaaaaaa
    28741 agaacttggt atgcttcaag ctattggtct tactaataaa caattagtga aaatgcttaa
    28801 ttcagaggca atatattaca cttctggaat gatgatagga agtattctat ttggaggtat
    28861 attagggtat attgctgtta tatttctaaa aaagactgga ttatcttatg caacatattc
    28921 tttgccaata gtaccaatct tgttgatgat tgtgtgtatt cttatagctc aatttataac
    28981 aacttatttg ataggacgaa gttttaataa agaatctctt attgatagag taagatacag
    29041 tgaataaaat gcaaatattt atggttagtt taactagcaa aaaacatata aaaaaatata
    29101 gttaaagaga ttcataatta aataggttaa agcatagtac agagcaaaca atttaaacag
    29161 atgtttgctc tgtattttat tatgtaatca ataatatatc taatatactg atattattca
    29221 tgttagaaaa tgtaccggta caattctaaa aaagtataaa attgtaccat ataaaatgaa
    29281 aattttgtat aataatcgta tagaaaataa ataggagctg atatatatga ataagaagat
    29341 taattatgca ccaggaccaa ctgaaactag ggaaaatgta agattagcaa gagcagaaaa
    29401 aacaacaaat ccagatatag atattgattt tgttgaattt tataaaaaga catgcgagaa
    29461 gtttggtaat ataattggaa caaaaaacga tgtttacata ttgagtggtg aaggaatatt
    29521 aggtcttgaa gcagcatgtg catcacttac agaaaaagga gatagagtac ttgtaattga
    29581 caatggtata tatggagaag gttttaaaga ttttgtaaca atgtatggtg gggagtatgt
    29641 gcttttttca agtgagtata ctaaaagtat agatattgat gaattgaaag catttttaga
    29701 tgaggatagt aattttaaat atgcaacagt agtgcattgt gatactccaa ctggcgtatt
    29761 aaatgatgta agcaaaatat gtccattact taaaaaatat ggaatattaa ctgtagttga
    29821 ttctgtagca ggtatggtag gagaaagatt aagtgtagat gaatctaaaa tagatataat
    29881 attaggtggt tcacaaaaag ctatttctgc accagcagga cttactatag tcggaataag
    29941 tcaagatgca aaaaattgta taaaaaatag aaaaactgat gtaataggtt tttattgtaa
    30001 tttaaatata tgggaaggct attatgagaa aaaatacttc ccttatacaa tgccaataag
    30061 tgacataatg gggcttgata aagcacttga taatatatta gaagaaggaa tagaaaatgt
    30121 attaaataga catgaaaaga tagcttatag tgttagaaaa tctattgagg aatatggtct
    30181 agaactattt ttagaagaag gatattctaa tacagtcaca gctataaaaa ttcctgaaag
    30241 tataggagct ttagaactta ctgattatat gcttaaaaat tataatacac ttgttgcaac
    30301 atcactaaat caatatatgg atactatttt aagaataggt catatgggag agaatgcaaa
    30361 tctaaataaa atagaacatg tactaaatgt tttggataaa agtttaagtg cattaggatt
    30421 caaaggaaat ggaaatttgt taaatttatt taataagtat tatttttaat agataggggg
    30481 atatagtatg gaagttacag ctaagcaaaa atttacaacg aaagacatgg tggaaacctc
    30541 actattaata gcattagtat ttattgcaac taaatttata aacataagat taccaatatc
    30601 tataaatggt gggcttgtgc atttaggaac tgcaatgttg ttcatatcag caatagtatt
    30661 tggaggtaaa aaaggtgctt tatcaggggc tattggtatg tcactttttg atttgatatc
    30721 tgggtggact ttatgggcac cgtttacctt tatagttaga ggagttatgg gttatttact
    30781 tggaaagatt gcatgggcaa atggtaataa tgggaataat tttttaatca atgtaatagg
    30841 aatatgtgtt tcatcaattt ggatgctatt tggatattat gtaacagaag ttatacttta
    30901 tggtaatttt atagtacctc ttacttcaat accaggtaat ttaatgcaag tattgatagg
    30961 tcttataata gcgcttccta tttctaaggt attgaaaaaa tgtataaaat aaatataaag
    31021 tgaactaaaa tttatgatag cataaattat actgtataat attaggatgg agtacataat
    31081 taaaaaattt tagtatagag gttagttcac aatgttatca gttacaagat attcaatcaa
    31141 aaatgttcgt ttaaagccgt gggtaaagtt tatttggtat tttgagacaa aatcaaatat
    31201 actgttaaat aataagttac ttccgacaga cagtatagat attattctca atctttctga
    31261 tgttatggaa tataaaattg aaaatcaaga ctatactgct agtaatatgc attttaatgg
    31321 aataagggat aaacatggat ttattattca acatggaaat atacgtgtta tagggatttc
    31381 tttttatcca tttggtctct atccattttt aaagatacca atttcagagt ttaacagaca
    31441 aattgttgac ctagaagcag tatcacagct atttgcaaaa aaattggaag agtcattaaa
    31501 tccaacacaa tcagtagaaa aaattgtttt atatttagaa gaagtattat tatccatatt
    31561 agaagaagat ttaatttcaa ataaatatgt aaagcttcta aattcattta tatatgttaa
    31621 tagatatagc aatataaaag cattttgtga tgatacaaat ataaatatta aaacattgga
    31681 aagaatctgt ttaaaatata cagggtatac acctaagatt ttaaagagaa tttacagatt
    31741 taaaatggca agcaatcaac ttatttataa ttataaagat gatgaacttt ttgattttat
    31801 ttatgaaaat gagtactatg accaagcaca ctttataaaa gaatttaaaa aattttcagg
    31861 gacgtcacca attaaattta ttggtgagaa taagacgatt aaagaaaata taaagtacag
    31921 ctatttataa ttatactgtc gattttttac aatacctcca atttattttt tgatatgata
    31981 taggaaaata aattggaggt tttacatatg aaaattatta cagaaatagt tgagtttaat
    32041 gtggaagaaa atttatcaaa agaagagttt atagaaattg taaatgactt agaattaaaa
    32101 tttcattcta ttcaatctgg ttttattgat actgagcttt tatatgatga aaagtctcaa
    32161 aagtggatta tgatacaaca ttgggaatct atagaagacc tcaagagtgc ttctagtaaa
    32221 atgtttaaag attcttcaac tgagaaattt agaaaagctt taaagaatca aactgttaag
    32281 atgaatataa taccacaaat taaaagttgg aaattataga acttaatgta aaagatagtt
    32341 aaatagtatc aaactaaatt agccaaataa cattttgtat ttaaattgtt atttggcttt
    32401 tagtgtttat tttaatatga gtgttatata ctagaaaaac caaccttaaa tactactatc
    32461 taaaagcact attgctgtac gagaggcaat aggcatttta gaagaaggag gagttgagct
    32521 aaagtataca tcataatctc ttaagttttc tttttttgtg tttcctccaa actctaccat
    32581 atcagtattt aatatcgttt tgtagtttgt attttgaaac tttgaaatat caactgattg
    32641 tattagatta gggtggaaat taaatataaa taaatatttg cccttactgt acaccaaaat
    32701 ctttttatca ttatccatat gtataagttc atgactattt tggataaaaa tatcactgta
    32761 ttttgtaagt tcgagcatag atttatcaaa atttaataat tgtttatact ttaagttgtt
    32821 atcttctgaa aggctccatt gtcttctggc atacttatag ctccaattgt tgccttctcg
    32881 tggaaaatca atccactcag gatgtccaaa ttcatttccc ataaaattta gatatccttc
    32941 acctgctaga gaaaaagtta ttaatttaat taatttaata agagatattg ctctattgat
    33001 aacatggtta tttgagttta tttccatatt ccagtacatt tccttgtcag ctagccaaaa
    33061 aataattgtt ttatcgccaa ctaatgcttg gtcatgggat tcacaatatc ctatattttt
    33121 ttctccaggt cgtctagtag taagttcata ccacattttt cccaaatccc agtcttcatc
    33181 agataagtta gagatagttt ttatccaaaa atcaggtact cccattgcga gtctatagtc
    33241 aaatccaatt ccgccgtctt ttatagggat acacatacca ggcattccac tcatatcttc
    33301 tgcaatactt atcgaattag gtttaatttc ttttattaat tcatttgcaa attggagata
    33361 tgttatagct tcaatatctg tattcatact aaaatatttt tcataactat caaatgaaac
    33421 tccaagacca tggttatgat ataacattga agtcacacca tcaaacctaa agccatcaaa
    33481 atggtactca tttaaccaaa attttatatt tgaaagcaaa aaatgtatta cttcaggttt
    33541 accatagtta aaaagttttg taccccaagc aggatgattt ccctttgaac ctgagtggaa
    33601 aaattgatgt tcacttccat caaactcatt tattccttct aaagtattct ttacagcatg
    33661 agaatgaact aaatccaata aaacagcaat ccccattgaa tgagcagtgt ttatgaggtt
    33721 ttttaagtct tcaggagtac caaacctaga tgagattgca taaaagtttg aaacttggta
    33781 tccaaaagat gcataatatg gatgttccat aatagccata agctgaatgg tattgtatcc
    33841 agcttttttt atcttaggaa gtattttttc agtaaattca ttataagttc caatagattc
    33901 gctttcagta gacataccta tatgacattc atagattagt ggataagtta tgttttttaa
    33961 gtcaaaatca ttgtcagtcc agataaatgg agtttttggt tgccatattt gtccattaaa
    34021 tccaccaaaa tctttttgaa ttactctttt tatatataat ggtatacggt caaatgtttt
    34081 tccatttgca gtaacttgta cctttacctc tgatttgtga ggtaagctat tttgaccagg
    34141 aataaatatt tcccaattac cagacccaat ttttcttaga ggatgagatt ttctattcca
    34201 attattaaag tcaccaatta gtgacaaact atctgcattt ggagcccatt ccctatagaa
    34261 gaatccatca tttgtctgat gaaatccata gtaaagatga gcatttgcaa aagaactaaa
    34321 atctttatag tttttcaatg tttttgattt catattctca taatgtttca ttcgcttttt
    34381 tatatcagat tcaaatggtt gtaggtatac atcaatatct aaaattttaa atcttttttg
    34441 acttttcatt gtatttctcc tccataataa atattacatc taaaaaataa tatttatctc
    34501 atactatctt ctctatataa ttgatatata ttatataatc ctaataaaaa gcttaagcca
    34561 ccaatgatta atttcataat ttacctccct agtatttata cttatatatc gtattcatca
    34621 aaatttaaaa gtttgtttaa aactctttta ataatatcat aatcaaagcc tttataggaa
    34681 agatgttgag aaattttttg atatattttc tttttatctt ctttctttac tctttcatac
    34741 cttttttttg ctagatacat agcattttca aattcagtgt ccttgtctaa ttcagatact
    34801 gcttcattga ttgtacttct attgatacct ttattatata aattctgctt aattctgttt
    34861 ttgccacatt tatttaggtt aacatttgtg ttaacaattt tttgagctaa tacactgtca
    34921 tttacgagat tataactttt aagaaaatct atgaccctct caattatatt ttcatcaaat
    34981 tctgatgata gtttttcttt taattttttt tcagattggt cagcatgaga taaaatattt
    35041 aatgctttat ttttagcttt tatatacatt tcatcatcta aaataggttt taattcttct
    35101 tcatttattt cattcccctt ttttaaatta aatgtataaa caagttcttt aaatatagca
    35161 ataaaaaatt tatcatctac ataaatatta actctatcat catttctttt ttgttgttct
    35221 atttttgtaa ttatacccat aagtcctcct tttttatatg taattatatt aacaaaagca
    35281 actaataaaa tttaacaaaa aacttatatt tgtatgttat attttatcaa aattaagaca
    35341 aaatagtaaa aagttatttt tattaaaaag taaagtagaa tcatgttaaa taattatttt
    35401 tttaagtgtt ttttagcata taaatataat tatataccca aaaaaacaaa ttataagctt
    35461 taagaaataa aaaataatgg tacaatagta ttataacatt aaaaggagac aaaatatgag
    35521 aagtgaaaat ctagttaaga tggcagagtt gaaaaatact gaaaaactag agaaatttaa
    35581 tcgccataaa ggcaaaatca aaataggtat attgggaggt acttttgacc ctatacacta
    35641 tgctcattta gcaacagcag aatttattag agacaagtat gacattgaca aaataatctt
    35701 tataccatca ggaaatccac cacataaatt atgtatcact acagataagt atgatagata
    35761 taatatgaca cttctagcaa ctgaaagcaa cgaagatttt ttagtttcaa aagtagaaat
    35821 cgaaagaaag aaaaggactt atacaataga tacattaaaa tacttaaaga aaaaatacaa
    35881 aaatgcagac atttatttta taacaggtgc agatgcaatt tgtagtgttg aagagtggaa
    35941 agatgtaaag aaaaactttg agttagctac ttttatagcc gcaactagac caggaattag
    36001 tttgttaagg tcacaagaga ctatagaaaa actaactaag aagtataatg ctgacattat
    36061 aacagtttat gttccatcac tggatatatc gtccacatat ataagggaac agctaaatga
    36121 aggcaagtca atacgttact tagtacccga aaatgtagaa aattatttat atgaaaataa
    36181 actatatcaa tatggagatg attaaatgaa cttagaaaat attaaccaaa gactaaatca
    36241 aatgctacca gtggggaggc ttagccattc aaaaaatgtt gcaaaatgtg ctgaaaaatt
    36301 atgtgagatt tatggatgtg acaaagaaaa agcttactta gcaggaatga ttcatgattg
    36361 tgcaaagtat ttaagtgaca aggaaataga ggattatgta aataagtatg aaatatattt
    36421 agacccaata gaggatggaa atcgttcctt gtctcatagc gtaattgggg cgtacatatg
    36481 cgagtatgaa tttgaagtag aagatgaaga tattataaat gcaatcaaat atcatacaac
    36541 agggagagag gacatgtctc ttttagaaaa aataatttac atagctgatt taattgaaga
    36601 aggaagaaaa ttcccagttg tagatacatt aagagaatta gcatatggag ggaagcttga
    36661 tgaagcactt ttaacttctt ttaataatac tttaatgttt gtcatcaata aaaaagaaga
    36721 gatacatcca agaacagtta tggcaagaaa ctatctaatt aaagaaaagt tattataaaa
    36781 aagctatata aaggcaaaca tattaataaa tttaaatttg actatgttac aaaatcgtat
    36841 taatatgtta caataactaa gtgtatttta attaggaaag tgaggaattt ttatatgaca
    36901 gtggaacaaa tgacaaaaat agcatatgat gccatagaag ataaactagg tcaagataca
    36961 gttataataa atataggaaa agtttctagt ttgtgtgatt attttattat tactacagca
    37021 tcttctcaga gacaggtaaa agctattgct gacaatgtgg aagatgaatt agcaaaacta
    37081 ggattagagc caagaggtaa agaaggacaa ggaactcaaa cttgggtact ccttgattat
    37141 ggagacataa tggttcatgt atttaatgag gaaaatagag ggttttataa tttagaaaaa
    37201 ttatggaaag atgcacctta tatagatatt gacacattag cataaacatg ataatatatg
    37261 ttattataat tagaaaattt caaaaattat attaaattga atagactaga gcagtaaaag
    37321 attatatttt ttcagagagt tagtggaagg tgagaactaa tattatataa ctttgaactt
    37381 actagattaa aatctattct ccatggctgg attaaaagct atctaagaga gtctatatat
    37441 gtagtaggct aattagggtg gtaccgcgaa gatttatcct cgtccctaaa cgtaagttta
    37501 gtgacgagga ttttttattt tcaaagaatt atttggaaat tataagtaaa taatactaaa
    37561 taaggtagtt atacacaatt aaaaatagga ggcaaaatat gagcgtatat aattttaaag
    37621 aagtagaatc gaaatggcaa aaaatctgga aagataataa tcagtataaa atggatactg
    37681 cacaaactga aaaacctaat tattacacat tagaaatgtt cccttatcca tctggaaaaa
    37741 tacacatggg acatgttaga aattattcta taggtgatgt tgtcgcaaga ttcaaaaaaa
    37801 tggaaggata taatgtactt catccaatgg gatgggattc ttttggatta ccagctgaaa
    37861 atgcagctat aaaacatgga atacatccgc ataaatggac aatggaaaat atagaagaaa
    37921 tgaaagagca attaaattta ttagggatta gttatgattg ggataaagaa gttgcaactt
    37981 caactccaga atactataga tttacacaag aaatattttt gaagttttta gagcatgggc
    38041 ttgcatataa gaaaaaatca tatgttaact ggtgtccttc ttgtgagact gttcttgcaa
    38101 atgagcaagt tgttcaagga gcatgtgaga gatgtaaagc aacagtatta aagaaagatt
    38161 tagaacaatg gtattttaag actactgagt ttgcagaaga attacttaat gacttagata
    38221 cattagatgg atggccagaa aaagttaaaa caatgcaaaa aaattggata ggtaaaagta
    38281 caggagcaga tttagttttt gatatagatg gaactgataa atctatgaca gtatttacaa
    38341 ctagaccaga cacaacttat ggagttactt atatggtact tgctccagag catgaattag
    38401 ttaaagaatt ggttgcaggg actgaatatg aagcagatgt tgaagctttt gttcaaaaaa
    38461 tgcatactat gacagaaata gaaagaacag cagctgatgt agaaaaagaa ggaatgttta
    38521 taggtagata tgttataaac cctttaaatg gcaagaaagt tcctctatgg atagctaact
    38581 atgtattagt tgagtatgga acgggtgcta taatggcagt tccagcacat gatgaaagag
    38641 atagagattt tgctgaaaaa tataatttag acataattga tgtaataaca gaagataaca
    38701 aaatgataaa ttcagaagaa tttaatggat tagatgcttc agaaggattt gaaggaataa
    38761 tcgataagtt agaaaaagaa ggtagaggaa agagaacaat taattataga cttagagact
    38821 ggttagtttc aagacaaaga tactggggtt gtcctatacc tgtggtttat tgtgatgaat
    38881 gtggaatagt tccagttaaa aaggaagatt taccagttct tttaccaaca gatgttgaat
    38941 ttactggaaa aggggaatct ccacttacga cttcaaagca atttatgtca acaacttgtc
    39001 ctcactgtgg aaaaccagct agaagggaag ttgatactat ggatacattt gttgattctt
    39061 cttggtattt cttaagatat gtggatagca ataatgaaaa tgaaccattt agtaaggaat
    39121 tagttaatag atggcatcca gtagaccaat atataggtgg agtagaacat gctataatgc
    39181 atttacttta tgcaagatgg tttgtaaagg catttaagag tatgggaatg gtagacttca
    39241 atgagccatt taaaaactta cttactcaag gaatggtact tatggatggt tctaaaatga
    39301 gtaagtctaa agggaataca gtatctccta tggatataat agatgagtat ggagcagata
    39361 ctgcaagact tttcgtatta tttgcagcac caccagaaag agatttggac tggtcagagc
    39421 aaggtgttga tggatgcttt agattcttaa atagggttta tagattggtt gacgagttag
    39481 cagatgtggt taaaaaggat gtagagtttg gagaattaaa ttcccaagat aaggatatga
    39541 gatatactat acactctaca cttaaaaaag ttactgctga tttaagtgaa aaattcggat
    39601 ttaatactgc tatatcagct cttatggaat taataaatga tatgtataaa tataaagagt
    39661 tagataatat aaacgaagca gttataaaag aaggtgtaca aactatagta actataattg
    39721 caccatttgc ccctcattta ggtgaagaac tatggactat gataggtaaa gaaggaagtg
    39781 tatttgatat agactggcct aaatatgacg aaaaagcttt agtaaaagat gaaatagaag
    39841 ttgttgtaca agttaatgga aaagttagag gaaaattaac tgttaactca aatatatcta
    39901 aagatgagat ggaaaaggta gctttagaag atgaaaaaat aaaaggatta gttgaaggta
    39961 aaactatagt taaagttgta gctgtaccta aaaaattagt aaatatagtt gttaaataac
    40021 tcaaaaatat gaacaaaaat ataaatttat aaaataaatg aggctgtctc aaaatagaga
    40081 taagcctttt ttcattgttg aaattataaa aatagatttg attttataca aaaaaatgat
    40141 taaagaacta aaaagttcat tcttatatac cctagttgtt gttttaaata ttattataat
    40201 taatgttaaa tcgagttaat gtattaagtg gtatgtaaat cattcagttt ccaaagtgtt
    40261 gttcgtcaag gaactgctct aagcttaaac gaaaaaaata taattattgt attttgaaat
    40321 ttttttctat ataatgaaaa caggcaaaaa aggtgcatat ctatgcttta tatgaaacac
    40381 ccagacaacg aaattcagat tcatataata gctaccatgt ttctaaaaag gaacttgtaa
    40441 aaaagatagc gtttcaactt cccagttgat taaaattatc agcaggaaaa caagtatggt
    40501 cagcagtggt attctaatgg gtgttgtcga tatatccaag ttgggattgt ctgaggatgt
    40561 tatcaaataa cttgtgatag gtgcagtaga tgtggagatt cctgatacgg atttattctc
    40621 aggggatggt tgcttcggga gatatggagc gtaaccagtt tattgatatg gtaaccattt
    40681 tttgtctaat ttgatgaaag aatcccaaga ataccataca gaagtaagac aggaattaac
    40741 tggtggtata aactgataaa ggcaaattta aaaattttga taggatgatt ttaagattta
    40801 tcttctattt tgttttttag aaataattga aatgttatga ggaggtatgt acaaatggag
    40861 actgtaacaa ataatcaatc aggtgtagaa attcaactca gtctgtttga agctatcaaa
    40921 gatttattgt taattaatcc tattttcttg ttgggaatta tcttgattgg tattttctta
    40981 gttgtaatat tcaagaaaaa tagtgatatt ccaaaggtca aaacatctat tttatcactg
    41041 gcaatgtatt actacttatg tttaacacta tcgcatattg ttggtatacc tacattcggt
    41101 gaatatataa gactgtcaca gttaggggaa acgttcttta atccaaacat aaatcttatt
    41161 ccttttagtg atggatttag tttgagtttt attttgaata ttttactttt tgtcccatta
    41221 ggttttttat gtccgcttat aagcaaaacc tttgaacgtt tgagaaatac attcttgatt
    41281 ggttttggat tatctctttt tatcgaaact attcagcttt ttacgttaca cagagcaacc
    41341 gatatagacg acttactcac gaacgtaatt ggaacaataa taggctattt ctgcttcaaa
    41401 ctgattgcta aattgcgaat tgtaaaacta cattcagatg atgactctat ggaacaggat
    41461 tacacagtat gtattccgat tgccattata atagttgccc ttatattagg attttttagt
    41521 taagttccaa atagctaatt tgggaatcca gacaatataa cagatactat gttacctttt
    41581 acaggagcat actatctgtt atattgtcaa catcctattt tatggtttgt gtgtagtttc
    41641 ctgtaaactg aactaaataa ttaaatagaa gctattagaa ttaataataa cagatagata
    41701 ttaataagca taaacaaata gtaaaatgtg tagatataga cgtttttaag tgaaactatt
    41761 ataaaataat tgactgataa tcaactgaca aaacctattt ttttaggttt aactgacatt
    41821 taactgacag tcagttttta ctatttaaat agaagttcgc ttagattgtc ggaagcttct
    41881 ttgtccattt ctttaagtac atgagaatat ctattcatag ttatttttat atctgtatga
    41941 cccaatcttt cagaaacaac tttcatatta gttcccccta aaagcagcag agtggcactt
    42001 gtatatctta aatcatgtat tctaatcttt tttaaattat tttttttgta aaattgtgaa
    42061 acgtttttct tagtgaagaa ggtagaaacg gatttaaatt ggtgtttaga caaactaagt
    42121 tatttctgtt ttccaggata ccttacaatt ttaatttgct ttgtcttagt ttttcaattt
    42181 tcaatttttc aattaattct tttggagctg ataaagctct ttttgatttt ggtgtttttg
    42241 gctctttaaa agatattttg ccattagaat agacaagaat ttgattaaca tttatcagat
    42301 tgttatctaa gtcaacatct gaccacctaa gcccaatagc ttctcacatt cttaaaccta
    42361 gaagtaacat taaaagaata ggtatttcta tattattacc aagtaacttt tctattagca
    42421 ataatacttc ttctctattg tatatttcat ttgtagaata tttttcttta gaaggaagtt
    42481 ttataaaatc acatgaattt tcttgtattt ctcttaatct atacgcttcc cttattacag
    42541 aacttagaaa gctatacctt actttagcac tagtaaaggt agaattatta aagttataat
    42601 ttacaaaagc ttgtactgaa ctaggattta cttctgtgag ttttagattc ttaaaaaagg
    42661 ttctatgtaa tttttaaccc aagattttgt atttgtaaca gtatatggag accaattatt
    42721 tttattcatg tctatatatt tataacatct ttctacaaat gtcatacctt ttggagcaac
    42781 aaacttatta ttatttatag tagactttat ttcaattaaa tgtttttcag catccttttt
    42841 ctttttatag cttccataac ttttttgttt ctttttgcca gtttcttatt ccacatatac
    42901 atagaatttt tcccatcttt ttcttataaa agctgatttg atattcatat atgtacgctc
    42961 cctttataag ttagttttat aaaagaacag ttggactgct ctaattatgt cattatcttt
    43021 ttaattcttt acagttacat tataacacgt atttgtatac acttaaatta acaggaagat
    43081 aaatatcgtt aaaggaggaa aagaaaattt aatgtagaat tatgtaagat atatagtatt
    43141 tcatatataa aagggggaga tattgtgaaa ttaaagaaat tattatcact agggttagcc
    43201 gtgtcaatgt ttgctgctgg tcctatatca gctaatgcac ttgatacaat ttatactatt
    43261 caaggaaaag ataaatatga aacggctgct cttatagctg atgaacaaaa ttatactaca
    43321 gctatattaa ttaatgcaga cagcacaatg gctgatggat taagtgcgag tggacttgca
    43381 ggagcaacaa atgcaccaat ttttttaact aagaaaaata acatacctaa tgcaactctt
    43441 aaaagagtag agaaagctaa aaaggtatac ataataggtg gagaaagttc tattgataaa
    43501 gcaacagaaa ctcttctaaa agataaagga atagaggcta aaagacttca aggtagtgat
    43561 agaattaaga caagctataa tgttgcaaaa gaaataaact caattaacaa agtaaataaa
    43621 gttatattga ctaatgcttt taaaggagag cctgatgcaa tgagtgctgc acctgtagca
    43681 gttagagata aagcagctat agtattaact gatggaaaaa gtgttccatt taatacaact
    43741 ggtgtagaaa gttatgtaat aggtggtact tcatcaatga gtgacaaact agttaatgat
    43801 actaattcaa ctagattagg tggagtagat agatatgata ctaataaaaa aatagtaaat
    43861 aagttctata atggcgcaaa agaattttat atagctagtg gtactgattt agtttatgct
    43921 ttagttgctt caccaatggc taaaaataat cctattgtac tagttgatat tggaagtaat
    43981 aaagatatac ttaaaaatgc tgcaaaagtg actgggatag gaaatctaag tgataaaatt
    44041 atgctagagt gtgaaaatac catcaataat attagtcttc cgtcttcaaa taaaaatata
    44101 atatgtggat taaagattga ttttgattca aagcacataa tttggcaaga taaaggaagt
    44161 tatcttgaat gttatgatga taaccttgaa tattatactg aaaaatataa agacccaaca
    44221 gttcctataa atatagaaaa tacaagtaca caaaatttag atgtaacaaa attaaaagtt
    44281 tatggagaag ctaattcaac attatatatg gggcaatatg ctaaaataaa aatggatttt
    44341 aatagtttcg gtgtattaaa gccaggagaa aaatgtacta tttatttaga tataatttca
    44401 ttagatccat caagaagcat gacattaaca ttttcttatg taggtgataa agaaaattat
    44461 aaatatgaac aaggattggc aagtagaata ttattttata acccagatgg agaagatttt
    44521 tttgaatata aataactagt gggtatgtaa attaataagg gttttataag tttaattttt
    44581 ttattttcgt tttatattaa ctatgtggta tgtaaatgtc tcgatattgc tcatatgttg
    44641 agttattata tttagtttca tatgaactat atggatttta aaattaaaaa tacttaaaaa
    44701 catttacttt aatagtaggt gtttttttga ttaaaagaat attattataa tgtaaaaagt
    44761 aataaatagg taaaatatgt aagaattata tgttataata tttttagcaa gaagatgtaa
    44821 tctacaattt atagagtgga gttcatgtat caaaaaatta tcctcccgac gctaagaagg
    44881 gaggtgaata tacatggata attttttaca aggcatacta gcaagtctat ctgctagctt
    44941 aatagtttat atagctagca aactatttag aaagcgtaaa aaaccactca aagcggcaac
    45001 taagagtggt tgggaatttg atttaaaaat cagattccat aaaactaagt aatttctaaa
    45061 ttatgaactc cactctacag caaaatagat tgtagttctt cttgctttta ttatatcaca
    45121 aattggtaca gatattcaaa aataatattt ttatgatata ataaagtcat agaaattttg
    45181 cagtgttcga ttttttgaag aaattaaggc ttaacagttg aaatataagg cattgaggac
    45241 ttgtgataag tgttatcaat tgcactactc atggttcact gcaaatttga gagatttgta
    45301 tatgtgtagg cattggaaat actcaattta ttttggggtt ttatattaac tatatggaat
    45361 gtaaatattt tttttatata taaacttcta aacttttaca aaagttttat attaactata
    45421 tggaatgtaa atacacttcc aacaagcatt gtagggttga agtctacagg ttttatatta
    45481 actatatgga atgtaaatta aatcgtattt ataattatat ctacacttag taaatgtttt
    45541 atattaacta tatggaatgt aaatccaata gaccctccaa taactccaga tattccttct
    45601 ggttttatat taactatatg gaatgtaaat aatgaactat ctaaaccaag gtcataattt
    45661 aattgagttt tatattaact atatggaatg taaattgtat ccaaaaaatt acaataatca
    45721 tacgaattgc taatataagc agtcaatata tgtgtacatt tatccatttt atctctaaac
    45781 atataaatta ttatccccct aaaaaacttt cataaaaaca aaaagaacaa tatctctttg
    45841 gcaactggct aacataactc ataagatatt tatataagtc ttagtcccta tagctttgcg
    45901 tcactagatt tctctagttt tgccgattta gttttattct ataactaaat aatacaatag
    45961 aattagttat tattcaacaa gattgtttga aaattgaata aatttatagg tatgaacgta
    46021 atttcacact taaaaacaga ctaaatacgt agaatatata gaaaaattta cctgtagttt
    46081 ttgtgataaa aagctcctat tattaattta agctagataa gtttgaggaa gtgacataaa
    46141 ttgaaagaag gaatatatta tgttgaataa aaaattacca gatgcagaat taaaaattat
    46201 gaaatacata tggaatactg gttataaaac tgtaatatca aaagatgttg cagattaaat
    46261 agaaaaaaca tatgcttgga agcatacaac tacaattaca cttttagcta gattaactaa
    46321 aaaaggtttt ttcatatcgc agagaattgg gaaacatgta cactatagag tattaataaa
    46381 agagaaagaa tatctcaaaa tagagggaaa aagaattttt ggaggtctac ataacaatcc
    46441 attatcagaa ctaatttcaa aattacatga tggagaagaa ataactgaag aaaaaataat
    46501 agaaatagga aattggataa aaagctggaa agatgaagat taataaataa aaggtagcta
    46561 ctaatataag tgctaccttt tttcttgtgg ataatattgg ggataagttg tggaaaattt
    46621 gtgtaaaaat tgtggataaa atattttgct gacatttaac tgacataagt attagatttt
    46681 gatttaaaaa tgttgaaata tcaataaata attaaataga agttattaga attaacaata
    46741 gattcctttt tcaaagaagt ctttgatttc atcaaggtta tcagtttttc caagtgctaa
    46801 gataagtttt attctagctt tttgacctgg taagtcccca ccaaatatac aaccaagatt
    46861 ttttaaatct ctaccagaac caaagtaacc ataactatcg aatactctac ctgaatgaca
    46921 tctagaaact ataactacag gaagtccttt ttctctagca tattcaactc cagaaagcat
    46981 ttgaggagga atattacctc tgcccattgc ctcaataact atacctttat aaccactatc
    47041 aacagcaaat cttataaagt cagcattttc tcctacatat gctttaaata aggcaacttt
    47101 agattctacc ttatcagtat ctataattgt tctatttaca atatctcttg taaggacaag
    47161 ttcgttgcag tcaattattc ctaatgggcc actagttaga gatttaaatg tatttagaga
    47221 aagagtattt gttttagtag cttcagaagc taataatact tcattgttta aaactacaag
    47281 gactcctttt cccatagcat ctttggatac tgcagtacaa actgatgcag ataaattact
    47341 tgaaccatca tatccaagtt cagaactact tctcatagca cctgtgacta ttacaggttt
    47401 gatgttgtca attgtaaggt ctagaaaata agctgtttcc tctaaactat ctgtaccatg
    47461 tgttattaca acacctgtaa tatcttttct tgctaataaa tcatttacat aatttttcaa
    47521 ttccatcatt ctataaggtg ttatgtgagg tcctggaatt tcatcaaaat taaaaacttc
    47581 aacatctgca actttatcaa tgttggtaac cattgatagt atttgttcac ctgataaagt
    47641 tggtatagct gctccgactt tatcatctac tgtcatagat atagttccac cagtaaaaac
    47701 tatggcaact tttttcttaa tactcatact attctctgcc tttcataaat aaaatttata
    47761 attccgatag gattatatca aaaaaagtag ttaaggtcta cataaactct tttattcata
    47821 taatttagat atttcagtgt aatataatta tttcttaatt ttttgccatt caacgatata
    47881 accatttaat atctctctta tggcttttcc tatcttagta taagcttcat catatagatt
    47941 tgctagagat attctaattg accattcagg gccatagaat ccatttccac taagtaaaac
    48001 tactgaatag tcattagcta atttataaag aacatctact ggagatgcag ttttacttat
    48061 aaatttaaat aagtcttctc cgtagtttaa tttagcccat tcttcaatat caaattgagt
    48121 gtaatatgca gcattgtttt tgttttcatt aattggaagc tctaaagctt caaataatag
    48181 tttttgacgt ctatggcaga tactcatatt aagttttttg taggcatcta ctttgtcaat
    48241 caatgcaaaa gcagagaaaa acgccatttg tacttgctgt ggagtagaaa gaccagcagt
    48301 atggttgaga gcaacaagtc tgctatcagc aacaactctt tccataaagg aaagactaga
    48361 agggttaaga ctcatatcac tgtaacgctt atctactgat ttttttaatt ctccagttaa
    48421 atcatttatt ttcttatcaa aaacattttt cttatgaagg gcaaatgtac caagtctcca
    48481 tcctgtaact ccaaaatatt ttgaataaga atatactcca acagtgttgt aaggtagttt
    48541 tgacattaag gatgaaaaat cttctacaaa agttccatat acatcatctg atattatcat
    48601 taaatcttta ttataatttt caattacatc tattagatta ttacaagatg tttcatccat
    48661 agctatagaa gcaggattat taggattaac aacgaataaa gctttaatag atttgtcacg
    48721 aagtttctct aattcttttt ttgtatattg ccatgtatga gcaccttttt catcaacttc
    48781 attagcattt atatttacca ctttaaagtc gtaacgagga aggttaggta tttcaagata
    48841 aggtgtaaat attggagtca ttaaggctat ggtatctcct ttttttagaa gttcatttgc
    48901 cattaatgaa tcaaatatat agcacatagc agctgtagca ccttcaacag caaaaatttc
    48961 tacatcacca tatttacctg atatatcata tttcatttct tgaactaggt aatcgtgtac
    49021 tattttttcc atatgaggta acattctatc tggaagtgga tagttatcac caataattcc
    49081 atcagctagt tcatgaacaa attcatctcc atcaaatcca agttcattta taccaaagtt
    49141 tataatatct ctagctaaat atgctccagg catatctata ttttcattta taaatttaaa
    49201 aaatctttct ttaataccct ttttttgagg cattccagcc aaatgaccta aatcccaatt
    49261 agaacgagtt tcagtaatag cgaaatgacc aaaagtaaaa aaggcttctc tagcagttga
    49321 acaagtccag tttggatttc cacgacctgc atcaagtaat tctctgttat tttcttttgc
    49381 agtgtatttt gcaatatcta ttaatttgtc cttaaattca aaagggctta tttttccata
    49441 tatgtttttg atttctttat aagtatgatt tttcatgtta aattcctcct tatgtaataa
    49501 gtttcacatt tttataataa aataaacaag ttgcaaaaat gattatactt taattataat
    49561 aggtttatag aaaaagtaaa gtatgaaatt a
//
LOCUS       DAMJWU010000017        49511 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMJWU010000017 DAMJWU010000000
VERSION     DAMJWU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.017"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            157..831
                     /locus_tag="PC460_001153"
     CDS             157..831
                     /locus_tag="PC460_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600046.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKGALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREKRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            819..1979
                     /locus_tag="PC460_001154"
     CDS             819..1979
                     /locus_tag="PC460_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2600047.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASTLTTFSYILLNNLYNSIQD
                     KYLKTPINTGFITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2250..2723
                     /locus_tag="PC460_001155"
     CDS             2250..2723
                     /locus_tag="PC460_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF2600048.1"
                     /translation="MLSFTFCSLPFWICYFWCGISDIFDGLIARKLRQQSGIGAKLDS
                     IADFIFSVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYRTFSSLHTLMNK
                     ITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELNRDIKSIFM"
     gene            3009..3677
                     /locus_tag="PC460_001156"
     CDS             3009..3677
                     /locus_tag="PC460_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600049.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3680..6148
                     /locus_tag="PC460_001157"
     CDS             3680..6148
                     /locus_tag="PC460_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600050.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGASENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTIAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTSISLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSILDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDNITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFATIFGSIAGYVSAIIINMATIEKLDFTNIPITSILQATIISILAC
                     IIATLIPLRKVKKMNIIDCIDVNL"
     gene            complement(6387..7811)
                     /locus_tag="PC460_001158"
     CDS             complement(6387..7811)
                     /locus_tag="PC460_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2600051.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7830..8522)
                     /locus_tag="PC460_001159"
     CDS             complement(7830..8522)
                     /locus_tag="PC460_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600052.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8577..9353)
                     /locus_tag="PC460_001160"
     CDS             complement(8577..9353)
                     /locus_tag="PC460_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2600053.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLFMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(9350..10120)
                     /locus_tag="PC460_001161"
     CDS             complement(9350..10120)
                     /locus_tag="PC460_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2600054.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10117..10821)
                     /locus_tag="PC460_001162"
     CDS             complement(10117..10821)
                     /locus_tag="PC460_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2600055.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11076..11588)
                     /locus_tag="PC460_001163"
     CDS             complement(11076..11588)
                     /locus_tag="PC460_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600056.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGYVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11914..12171
                     /locus_tag="PC460_001164"
     CDS             11914..12171
                     /locus_tag="PC460_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600057.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12314..12940
                     /locus_tag="PC460_001165"
     CDS             12314..12940
                     /locus_tag="PC460_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF2600058.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13187..13966)
                     /locus_tag="PC460_001166"
     CDS             complement(13187..13966)
                     /locus_tag="PC460_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2600059.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNRCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(14034..14495)
                     /locus_tag="PC460_001167"
     CDS             complement(14034..14495)
                     /locus_tag="PC460_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2600060.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14471..15148)
                     /locus_tag="PC460_001168"
     CDS             complement(14471..15148)
                     /locus_tag="PC460_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2600061.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15381..15767
                     /locus_tag="PC460_001169"
     CDS             15381..15767
                     /locus_tag="PC460_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2600062.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15770..17569
                     /locus_tag="PC460_001170"
     CDS             15770..17569
                     /locus_tag="PC460_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF2600063.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSSNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILKDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLNKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17924..19798)
                     /locus_tag="PC460_001171"
     CDS             complement(17924..19798)
                     /locus_tag="PC460_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2600064.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19803..21467)
                     /locus_tag="PC460_001172"
     CDS             complement(19803..21467)
                     /locus_tag="PC460_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2600065.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(21481..22302)
                     /locus_tag="PC460_001173"
     CDS             complement(21481..22302)
                     /locus_tag="PC460_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2600066.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKNILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDVVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22401..23165)
                     /locus_tag="PC460_001174"
     CDS             complement(22401..23165)
                     /locus_tag="PC460_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2600067.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23162..23911)
                     /locus_tag="PC460_001175"
     CDS             complement(23162..23911)
                     /locus_tag="PC460_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2600068.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24415..25020)
                     /locus_tag="PC460_001176"
     CDS             complement(24415..25020)
                     /locus_tag="PC460_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2600069.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            25199..25837
                     /locus_tag="PC460_001177"
     CDS             25199..25837
                     /locus_tag="PC460_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2600070.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            25969..26631
                     /locus_tag="PC460_001178"
     CDS             25969..26631
                     /locus_tag="PC460_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600071.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     FLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            26631..27812
                     /locus_tag="PC460_001179"
     CDS             26631..27812
                     /locus_tag="PC460_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2600072.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(27993..30653)
                     /locus_tag="PC460_001180"
     CDS             complement(27993..30653)
                     /locus_tag="PC460_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600073.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(30650..31333)
                     /locus_tag="PC460_001181"
     CDS             complement(30650..31333)
                     /locus_tag="PC460_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600074.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(31677..32630)
                     /gene="blaCDD"
                     /locus_tag="PC460_001182"
     CDS             complement(31677..32630)
                     /gene="blaCDD"
                     /locus_tag="PC460_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2600075.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(33171..35285)
                     /locus_tag="PC460_001183"
     CDS             complement(33171..35285)
                     /locus_tag="PC460_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDF2600076.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
     gene            complement(35369..36205)
                     /locus_tag="PC460_001184"
     CDS             complement(35369..36205)
                     /locus_tag="PC460_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600077.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            complement(36789..37151)
                     /locus_tag="PC460_001185"
     CDS             complement(36789..37151)
                     /locus_tag="PC460_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2600078.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(37385..37849)
                     /locus_tag="PC460_001186"
     CDS             complement(37385..37849)
                     /locus_tag="PC460_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2600079.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GLYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(38278..39699)
                     /gene="nhaC"
                     /locus_tag="PC460_001187"
     CDS             complement(38278..39699)
                     /gene="nhaC"
                     /locus_tag="PC460_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2600080.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(39729..40793)
                     /locus_tag="PC460_001188"
     CDS             complement(39729..40793)
                     /locus_tag="PC460_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF2600081.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(40778..42187)
                     /locus_tag="PC460_001189"
     CDS             complement(40778..42187)
                     /locus_tag="PC460_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF2600082.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(42187..44391)
                     /locus_tag="PC460_001190"
     CDS             complement(42187..44391)
                     /locus_tag="PC460_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2600083.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIIMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(44385..44759)
                     /locus_tag="PC460_001191"
     CDS             complement(44385..44759)
                     /locus_tag="PC460_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF2600084.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(44837..46267)
                     /locus_tag="PC460_001192"
     CDS             complement(44837..46267)
                     /locus_tag="PC460_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF2600085.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(46260..46568)
                     /locus_tag="PC460_001193"
     CDS             complement(46260..46568)
                     /locus_tag="PC460_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF2600086.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(46689..47756)
                     /locus_tag="PC460_001194"
     CDS             complement(46689..47756)
                     /locus_tag="PC460_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF2600087.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKINFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(48012..49385)
                     /locus_tag="PC460_001195"
     CDS             complement(48012..49385)
                     /locus_tag="PC460_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2600088.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYTLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT        16777 a         8353 c         5902 g        18479 t
ORIGIN      
        1 tttctaataa tttcatcctt gatttattta atatataaaa atatactaac tttaatgatt
       61 aagttttgaa atataactaa gaatttgaga ttttattgag atttttatac taaagtatac
      121 atactataca agcttaattt agaagggagc attcttatga acaataaaat actcgtcgta
      181 gatgatgagg aaggtataat aacactctta aaagattact ttgaaatgaa tggttatgag
      241 gtttacactg ctcaaagtgg aaaaggcgca ttaaaaaaaa tatccaaaaa tccagatatt
      301 attctacttg atataaacat gcctgaaata gatggaattg aagtttgtaa tagaattaga
      361 gattatgtat catgccctat tctatttctt acagctagaa ttgaaaatac tgacaagata
      421 aatggttttc gtgctggtgc tgatgattat attgtaaaac cttttgattt agacgaactt
      481 ggagctagag tatctgctca tttaagacgt gaaaaaagga agtcaaatca aactaaaatt
      541 aaattttata atgatttaac gatagattac gaaaaaaggt taatttgtat agataataat
      601 cagatttcac tttcaaaaaa ggaatttgat atagtagaac ttctctctat gaatcctgga
      661 caagtatttg ataaagaaaa aatctatgat agagtctggg gatacaatag caatggaaat
      721 agtgatgtta taatggaaca cattagaaag atacgaatca aactatcaaa acatactctg
      781 gaaaattata tagaaacagt ctggggagtt ggttataaat ggataaaata aataatatgt
      841 cattaaaaaa agctttcttt gttcttactt tatcttcact aattatagca tcaacactta
      901 ctactttctc atacattttg cttaataatc tatataattc tatacaagat aagtatttaa
      961 aaacacctat aaatacgggt tttataacca tagttcaaaa caatgtgaac caatttagtg
     1021 attatgataa aacgttactt aatataatca acatacttca gcttgtactt ccacttatat
     1081 tttttatagg acttttatta ttagctgata taatttttta caaggtaaaa ttaaaaacac
     1141 ccatagaaat actcaataaa ggtgcaatgg aaatatccaa caacaattta gacttttgct
     1201 tagagtataa taataatgat gaattaggta atctatgtaa tgcttttgaa aaaatgaggt
     1261 ctgaactaaa taaaaacaac ataaagatgt ggactatgat taatgataga aaacaactta
     1321 atgctgcttt ttctcatgat ttaagaaatc ccctaacagt tctaaaaggt tactcaaact
     1381 atttaacaaa atacatacca acaggtaagc ttagtgatga gaagatttta tcaaccacac
     1441 aacttatgtc tgagcatatt aacagaattg aatactatgt agaaaatatg agtaatgctc
     1501 aacgattaga agatttagtt gtttcaaaat ctatgtcgaa tataaacaag tttatagaaa
     1561 acttagatga aaatatatct atcattgcta aacaagaagg aaaatctttt acacttaaaa
     1621 gtcaaataaa taatataaat ctattttttg atgaaaatat cattcttaga gtcgtcgaaa
     1681 atataatatc gaatgctttt agatatgcta gaaataatgt atccatactt atatatcttg
     1741 aacaagaact ccttaccttt gtaattgaag atgatggtat aggatttagt aaggaatctc
     1801 taaaattagc tttaagacct ttctatagag ataagacttt aaatgacagc aatgcacact
     1861 ttggtatggg tctttatatc tcaaaaatat tatgtgaaaa acatggtggc tctatttcta
     1921 ttgaaaataa ttctactaac agtgctaaaa ttactgctaa attctcaaca agaaattaaa
     1981 atgattttgt aagctttcac tgctattata taaaatttgt tagtttagaa tttataaaat
     2041 gtatttcaat ttataaccaa tatatatact attttgtaga aattgtaaga gttgcttcaa
     2101 ttataatatt ttggcaaaag gcatttgaaa caataataga aatatatatt taaatatgta
     2161 gaatagtatg aaattagaat tttggaggtg attatattaa aagttttgct aataatatta
     2221 cattctctcg tattgtgttt gcattgctaa tgcttagttt tacattctgt tcactgcctt
     2281 tctggatatg ttatttttgg tgtggaatta gtgatatatt tgatggtctt attgcccgaa
     2341 agttgagaca acaaagtgga ataggtgcaa agttagatag tattgctgat ttcatttttt
     2401 cggttacaat tttggttact gtcggaaaaa atattgctct tcccatgtat ctatggtggt
     2461 gtattgttgc aatagcattg ttacggttga ttagttacag tattggtttt tttaagtatc
     2521 gcacattttc atcgcttcat actttgatga ataaaatcac tggaatttct atctttgcgt
     2581 ttccattact ctatatttgc tttggtcttg atgttgcagg aattatgata tgtatcgtgg
     2641 cttttctttc ttcttttgaa gaaatagcaa ttatgataaa atcaaaagaa ttaaatcgtg
     2701 atataaaaag tatatttatg tgaaaactta gaatttgtga caaaaataat ttatataata
     2761 atgaggaagt aaaacataaa aaattatcca caagtttatg tgtaaaatag ttttaaagca
     2821 atagagttgt attgtaaagc ctttagtgca taaattacat tttaaattaa aaatgatact
     2881 gaaactgctt atcagcactg cgaattatcc gcaaatagtg aagacttttt agatttaagc
     2941 taaaagttgg gaaaaaattg agttttaaaa cttacactct tcttatatag aaaaggagag
     3001 tgttttttat gattataaaa gcaaaacaat tatcaaaaat atatggttct aataacaata
     3061 aagttatcgc acttaataat gtaaatttag aaataaactc aggtgaattt gtttctgtaa
     3121 ttggaccttc tggaagtgga aaaagcacac ttttacatat tttaagtgga ttagataatc
     3181 ctacctctgg acaagtgtta cttgatgata aagatatata taaacacact gaaaaagaac
     3241 tatctgcact tagaagaaaa tcctttggat tcgtctttca acaatttaat cttttacctg
     3301 ttctgacagc atctgaaaat atttcaatgc ctgtacttct agataaaaaa caacctgata
     3361 aagaatattt aaatgaaatc tcatcattac ttggaattgc agatagactc aatcatcttc
     3421 cccatgaact ttcaggtggt caacaacaaa gagttgcaat tgctagagca cttattgcaa
     3481 aaccagatat aatatttgct gatgagccta caggaaactt ggacagtaaa agtggcagtg
     3541 aagttatgaa tcttcttata aaaacttcaa aacagtttgg aaagacttta gtggtaatta
     3601 ctcatgatga tagaattgca aaacttgctg atagaaaaat ttcaattatt gatggtgttc
     3661 ttatggaggt gaaatagaaa tgaagaatta cctttcactt tcaataaaag aattaaaaag
     3721 ccagaaactt atgactacat tcatcattat agcaatagta ctatcaacta ttatgacaac
     3781 tgtggttgga cagtcaattg gtatcctaca aaatttaaga attgaacaag cccgttcttt
     3841 taacggtgat agacatgtat cctttcacca acttactaaa aatcaagttg atgacataaa
     3901 gaaagatgat agagtttatc aagctggtac ttcaataact cttggcagta gtaaaataaa
     3961 ggattctggt atctctgtac ttgtaaaaga gtatgataaa acaggacttt ccaactaccc
     4021 caaattaatg aaattaaaat ctggaaattt gcctaaagat aaaaacgaaa tagccttaga
     4081 tgaaaacaca ctaaagctta tgggtataaa accaagacta ggagtgacta tacctatgaa
     4141 tttagatatt tcacttttaa atgatacaat accaccatat aattacacag ctaactttaa
     4201 attgagtggt attttagagg atgattatac aggatatgta agtggtatag taaacggtat
     4261 tgtaggcaaa ggtgcatcag aaaatctatt acctaagaga tatattttat cctctttgga
     4321 ttttaaaata aaacagcaag agaagtttca agaaatagta aatcaattag ctaaaaaaat
     4381 aaatctttct cacaattcaa ttcaatataa ttggatttac ttaaatgctc ttaaaataca
     4441 gtttgaaaaa gatgaaaata gttctaatag tgatggtata tctatgatta tattggtatc
     4501 tttatttgtt gccttattgg ttttactggc ttctggactt gtaatctaca atattttaaa
     4561 aatctctgtt acaaagaaaa ttaaggagta tggatgttta agagcaatag gagctgaacc
     4621 aaaccagata tataagatag ttattttaca aatcttaatt ttatgtacta ttgccatacc
     4681 tattggagct gtaattggaa ttatatcttc taaaggcata actggaatgg tcactaatat
     4741 cttaaatcca gatatacttc tagcaaatga taataaagaa attactgagc ttatacataa
     4801 aaacacaaca gcatatatat tcccattagt attaagcact agtatatctt tgattttttc
     4861 acttatttca gcattaccat cagcaatata tgcttcacat gtctctccca aaattgctat
     4921 ggctggcagt actaccaaaa tcaaaagaaa aattaaacga gaaaaaacaa taaaaaactt
     4981 tgaaagacat ctagcctggc taaacttaaa aagaaataaa ggaagaacta taataaccat
     5041 actatcatta tttatgagta taacagtatt cgttgcactg agtgagttct ctaatgtatt
     5101 agatgtatct aggtcagtaa gtaatttaaa agaaggagat ttttccttaa caaatgaaat
     5161 atctggtttt gataagtcaa tactagataa aatagataaa atgaaaaacg tcaatcgcac
     5221 ttcctttata aagtactctg aatacaaaca gggcgaaata gatacagata taaattttga
     5281 aaatagtggt gagatgttaa aaatcattgg aattgatgag caaactttaa aagacttaat
     5341 gccttctatt acagattcta tactagaaga ctttaaaaat ggttctatct gttttataaa
     5401 aaatccgatt gctatttcta caccaggtgt aaaaactaga catacaaatt taaaacctaa
     5461 agataatatc acaattaaca aaaaacaact agatatatat agtacagtag ataaaatgtt
     5521 ctttttacaa ggtaatggct ggataaatgg tgttgatgtt attgtatatg attctgttta
     5581 taacaccttg acaaataaaa acaagttcaa ccagataaat atttatgcta aagataaatc
     5641 aaaattagaa caaataagat tgtctattga acagatttgt gaaaataatc ctggctctca
     5701 ttggatatca tatattgaat cagataaaca acttaaagag agttttaaac aaatagaatt
     5761 tctagcatgg gcagttatat tatttgtggg attgattggt actttaaaca tcattaacac
     5821 aactcataca aatataaata ctaggactaa tgagattgga gttaaaagag ctattggtat
     5881 gagtaatagt agcttatata agatgttttt atgggaaggt gtttattatg gaatttttgc
     5941 tactatattt ggttcaattg ctgggtatgt cagtgctatt attataaaca tggcaactat
     6001 agaaaagctt gattttacta atataccaat tacttcaatt ttacaagcta ctattatttc
     6061 tatacttgca tgtattatag ctactcttat tccgttaaga aaagttaaaa agatgaatat
     6121 aattgactgt atagatgtca atttataact attttacaga taataaataa attaaattat
     6181 caatgactgt ctctattttc ttttaaatct aactaataaa tctttttaac tataatataa
     6241 gctttaaaaa aggcctgtct caaaatagag ataagccttt tttcattgtc gaaataatga
     6301 aaatatattt aacttcacaa aaaaagagtg cctcacgaat taaaaatttc gctttgagac
     6361 actccccttt aatttaaaat atatagttaa atgctatgcc aaaatttcac acatgctcca
     6421 cctgattttg aattttctat tttaacagaa cctccaagct gttccactaa ttgttttaca
     6481 atgtaaagcc caagaccaga atgtcctcct ttagtctgcc tagcttcatc tcctcgataa
     6541 aacttatcaa gagccttttt taaatcttta gagctaaacc ctctaccaga atcacaaatc
     6601 tcataagaaa tacaattttt ttcatccttt acagtaatac taatattacc acctgatgga
     6661 gtgtattgca gactgttgga tatgatatta tcaaaaattc gtgtcaacct atccacgtcg
     6721 atttgaattg gggattgtat attcccctgc attttcaata taagttcaac ttccttttga
     6781 tgagcctgta attcatagtc ttgtaccttt tgcctcaaaa attcaggtaa atttatagga
     6841 acaagattta attgtatatt tgacttttct aagtcagacg tatattgcat cttttggaca
     6901 agagcagcac ttttttctgt attttctctt atcactgtaa gatacctatg aagtttttca
     6961 ttgtcatctg tattattacc aataagtgca tctgtatatc ccaaaataat agaaagagga
     7021 gattttaaat catgtgctag agcttccacc atttcaacac gttcctgctc catcttccac
     7081 tgtgcagata aagagccttt taattcgtct ttcatttcag aaaatgcact acaaagttta
     7141 ccgagttcat tatctgaata atagtcgatt tcaaaatcaa ggtccttttc ttttatcttc
     7201 ttggacgcat caatcaatag atgtaatggt tgattgatat tctttacaaa gcgcttagaa
     7261 agccatctag taaaacccac gatatataga aatggagaaa acagagccat tataataact
     7321 gcaaatacaa atctccctct aatatttgca aaggtgattt tcacttggta gaatagaata
     7381 acagctccct caatctttcc attatcccct ttgattggaa cagtatggat ataaccacct
     7441 ttacgaacag ttttattgat aaaattatta aataattctt cttttgtttt aaatagtttt
     7501 ttggggttag taccatataa aatattgcca ttattatcca caacttgata tagcatatca
     7561 tccccacgaa tcgttctttt taaaccttct tcattcgatt gtgataacaa agttatgttt
     7621 ttttcattga tgtatttttc aattctagga acttgtcgct cataatagtt tggtggatag
     7681 atatccttat ttaaggaatg aatatacaaa tagagagcaa atacataagt aatcactgtg
     7741 gcaactatac tggctattat aataaaaata aaagtatgtc taaattgggt aataagggat
     7801 tgatttttca tttaataacc tctctctctt tacattttat tccacttata cccaataccc
     7861 caaactgtgg aaatatattc tttttctggt gtcacagcag aaaacttagc tcgtattttt
     7921 tttactcgtt caacaacagt tgaggaatca ccttcagaat cacaccccca tactttttca
     7981 tagatttgtt ctcttgagaa aacttgtcct gcatggagtg ctagtaattc aacaatatca
     8041 tactctattt ttgttaaatc aatattctta ccatcaattt taattgcacg ttccttcata
     8101 tataagcata acttcccaaa atacaaagta gatctcttgt tctcttcatt gatatactgt
     8161 gaacgctttt ccctacgcag attagcttca atccttgcca taagttcacg cagtccaaat
     8221 ggctttgtta cataatcatc ccctccaaag gtcaaccctt tgattttgtc ggtttctgat
     8281 tgctttgcac ttaaaaatat aattgggcat aaaacatcat ctctaattgc ctgacatacc
     8341 tcaaatccat tcattttagg catcataata tccagtataa taaggtcagg ctcctgtttt
     8401 gataactcta tacctgcctg accatcataa gctatcaaca cttcatgtcc ttttgccttc
     8461 aattcatcct caagtagcat tactaggtcc atttcatcat caataatcaa tattttactc
     8521 aatataacac cacctttctc ttatgggaag gtcagtatta aaacaatatt cgtctactaa
     8581 taacttttcc ttccctccca cattttaaac caaataacgc ctcctatcaa gaatataatc
     8641 aaacctattg ctactactat aagctctata ctagctgttt gcatcacttt agagtaaaat
     8701 gcctcagttg aaatagttcc agcttcttta ataaaataaa ttcctggaag catacctagt
     8761 tttactggcc atgtccatgg aacaaatgcc catacttttt caccaacgct agttaatcca
     8821 agtattgctg ccatcaaaat tccaccgata ctaataccaa tagatgcacc cataccccat
     8881 gcaaaactca cccaaagatg aatagctaat agtggaagtg tcccaatcac cacaagtcct
     8941 gctgctgaca ggaataacca tattgttgct ccagatggca ctgcaatact cattcctatg
     9001 caaagtatca acgtagcaat aaatgtgcat atagttaggc aaaacagaag aaaaagaaat
     9061 tttcccagat ataatctaac acgagaaata cctacacata aaaaaccatt aaaatttcca
     9121 gcaagctctt cctcctgcac tataaatcct gctaaaatac ctactccaat aggaatgatg
     9181 aatacagtcc atactgtaaa aaatccttca aaagcaaatt cttgtgtact tccagattta
     9241 gttgtgagat aaaaaaccac acataatgaa aaaataatag gcataaaaag agtaagccac
     9301 tggacagctg tcctctttgc tcgaagccat tctgatgata atagctttat catttgtcat
     9361 ccctcctatt gaaccaagct gcagtaagtg ctgttaatac tataaacaca accaaagaaa
     9421 caacaatccc aataggaatc actgaagtat tcaaaagtgg gtctccagct tctaatatag
     9481 taccgttagg atgtatgttg atgattgggc ataccattct aactgcccaa ctccaaggta
     9541 ctgcaatcca aaatggtttt gtcgcagcaa ggacaccaaa tatcatacct aaagctccaa
     9601 ctcccatact taggaacata cctttccatg tagctagcca tagctgaagt ggaagtatag
     9661 caagagaaac aacccagcat acaatacttg cccctataat ctgtccaaaa ggaactggac
     9721 cagctttcaa tattaaacca gttataattg ttacgactat taaaactagc gttgaaataa
     9781 aactgtagac tgccatagct ataactttat taatccaaag tgtcatagga gacacattgt
     9841 gtgtacgtaa agcacgataa tttccagctt ttttttcctg gaaagctacc aaagtagcaa
     9901 acaatgcaaa tccaataggc agaaataaaa atgaccacca gttaaaaatt aatgctaaaa
     9961 gactttgcca cgcccaagaa gttgtttgag ataatggatt ttgcatgagt gttgattgca
    10021 ttataaatgc atatgctgca aaagacactg ggaaaaaaac aataagttta cccataaatg
    10081 ttcttttata ctttaaaagt tctgattgta aagcattcat ctgttttgtc ctcctttaag
    10141 aataacatct gtaaaaaatt cttctagatt ttcattaggg tcaggcattc cttgaaataa
    10201 tagtcgtcct ccatttataa ttcctatatt atctacaact tgagcaacct ctgatagaat
    10261 atggctagaa agaattacag ttataccttt ttttggaaaa gaagcaatca attcacgcaa
    10321 ttcttgaatg ccaaaggggt caagaccatt ggttggttca tctagtatta acaactctgg
    10381 gtcactcaaa agtgcaatag ctattcctaa cctttgtttc atacccatag aaaattgtga
    10441 agctctcttt ttacctgtat ttttcaaatt tactatttcc aacacttcat aaattttatc
    10501 tttaggtatt cccaatattt ttgtatgtac taacaaattt tcctctgctg taaggtttcc
    10561 atacaatgca ggggattcta ttaaagaacc tatttttgca agagattctc gtttccaagg
    10621 ctttctatca aaaataatac gaccatttgt tgggcgaaga agaccaacaa gcattttaag
    10681 tgttgtggac tttcctgctc catttggtcc taaaagtcca tatatactat ttcgttcaat
    10741 ttgtagagaa acattgttaa ctgccatttg ttctccatat ctttttgata agttttttgt
    10801 ttcaagaatt aaatctttca tattaatttc tcccttcttg taatacctta aatctgttac
    10861 taagagaata ctaacatgaa aaaataacaa ttctataaca cctactaatt ttaataaaat
    10921 ttaaaataaa aaatagagtt accaatttta ttttggcaac tctatttaag tttatgtttt
    10981 acagttatat tagatttaag cttagtttca ttatcacact aacttcaaat ttactctata
    11041 atccttaaat caaacactaa tttattaatc aaacactaat ctattaattt atatttcttt
    11101 agacatctaa tatagtttgc tactaaataa taattattta tatcatattt atctaatcct
    11161 ttaaaagaag ttatttcatc attaagctca tcactacctt ctacaaaaac ataacctgtt
    11221 tcaaattcca tacctacaat aatcttacag ccactataaa tttcctcttg agtatcgtac
    11281 aatttttttg catttccttc atttggaatt gtaaatacaa taggttttaa ttctttatca
    11341 ctggaaatct tttgtgaaaa aggcttgttt aacttaatat tattgataac tacatgttta
    11401 aattcctctg aagtttcttc tactattaca tattttcgct ttaaaaactc tattatttct
    11461 aaaacagact tagaatttac ttttaaaata cttttatatt tttcatataa agatttccac
    11521 tgttcaattt tttcctgtgg tacttcttgg atatgtgtac ttattttttc ttgagatatt
    11581 tttttcaatt aaactctcct ttcaatactt aaatatcctt ataaatctca catgactaaa
    11641 gttacaaggt tcctacttaa tagaattttg catacttaaa tatgtcttat taattctcta
    11701 caggatatct ccgtagttcc tacgattctt acataagtta cttattttaa attgttatta
    11761 gttagattat aaaaattatt tattttatca ctaatataat actcttaaaa actttacaaa
    11821 tcaatttaaa tttgctacct tttatttata acaacttata gtacatttct cctatgcttt
    11881 tcaatatatt acttttaaaa ggaggttttc attatgattt taaatagagc tgagcttttc
    11941 cgaggatatt ctttaaatag atttaacgag atatgcaata tattatcagt aaataaaatt
    12001 aaatataagt ataaagtgaa aaataatgct tactctaaaa aacctttttt taatgaaata
    12061 acacttggca atttaggcca aaaggaagat ttctcttatg agtacttaat ttatgttcat
    12121 aaagacgatt atgaacaggc agagtctcta ataaattcta agttgcgata gattatctat
    12181 aaaacatgac attctgttgt catgttttat atgttattat actttcaaat atgctactat
    12241 attttcaaaa ttgtgtgctt taaaactagc tttttttagt aatttaaagc ttaaaactaa
    12301 ggagactcgt tatatgaaaa taaatataaa atcaaagtta agtgaattta ttaaacagaa
    12361 caatcttttt gatgacagca gaataatatc ctatcttcct aatataacaa tagaaacaga
    12421 caaaataaaa actataatga ttaatgaggt tgtcccaact aacaccaatg acgattttta
    12481 tagtgtagat aaagatgctg attatttaaa aactactatt cctttatttg acagtgcagg
    12541 aataaaagta tctaacataa atgatatcct agatatgggt atctatataa ctaatgcagt
    12601 aaaacttcct aaatcagaat atacaataac tcgtgataca attaaattac atatgcctat
    12661 tttagaagaa gaaattaaat tatttaaaaa cctagaggta gtaatgttaa tgggagatgt
    12721 tgcaaaaaaa tcttttaata tgattactaa aaagcatata aaaaagaatg tcatcccttc
    12781 catttcaaca tataaacttc gtaataatga actttattat gataatttaa gagtatttcc
    12841 atcatatatt atgactggtg gaaacatact gattgaaaaa tcaaaatttg aaatggcatc
    12901 agaagatata aaaaaaatgt tcgagattat aaacggttga tattacaaaa taataaaaac
    12961 taatataaaa taaaaagtgc ctcaaaaact tctatttatg aggcactttt attttatatt
    13021 agtgtaacta tatactatta acataattta ttatttatat tgttcctttt aatctctatt
    13081 ccatttagtc taggtaacat tactctttac aagattactt tatatttctt ttatatctct
    13141 aacgttttta tttaataaaa caattattaa tacaagtcaa atatatctat ttatctaaaa
    13201 atatatcttt tacagtaata tctggatggt gatacttctt cctattcgtt aaaaaagatt
    13261 tttttacatt aatagcctct tttggacacc agtgaaaaca agctaggcat ctttcacact
    13321 cattacccca ctttggcttt ttatcaacta tacttatatt ctctacagga caaacccttt
    13381 tacatgtccc acatctatta catttatttt ctgcaaaaaa tttagtagtt gcaatttgca
    13441 tattcttacc tactaaacga agtacaacac tatctatatg tgactttaaa ttgttttttc
    13501 cttctatatt tccaatctcc aagttattga tacattttgc aatctcagct accctattag
    13561 tctgattttt caatctttct tcttcaacat ctggaggtgt tatcaaagca tttccaggca
    13621 tattgattaa aaaaccagca gataattttt tccccctttt tttaagtaac ttatctatag
    13681 taaataaact atgtccagct accccattac aagtggcaat ggcatatata tgagtattaa
    13741 tattaaaatt taatttctct acaaaagact ttactatatc aggaacatca gcaaaataaa
    13801 caggaaaaac aattaccaat atatcttcat ttatgtctat attattttct tttctatgta
    13861 tagctaaggg tataacttct ttacttccat ttaacgtttc ttttaaaata tttgaaacta
    13921 ctagcgaatt tcctgttcca gaaaaataat aaatcttact attcattctt atccctcttt
    13981 ctatatttct acatcatcaa tagttaaaaa gtctcttaaa ataggattta tatttacttt
    14041 ccaattctat ccaaaatatc agtaaacaaa ggcaattctt catcagagat agatttaaat
    14101 gtctctgttg catatatact tgcaatttca tttaatggtt cttctaattc tctacctaaa
    14161 tctgttagga atatttgaaa cttacgtcta tctttaacat ctactttacg gacaattaat
    14221 cccttatttt tcataacatc taagattctt gtcgtattag atttatcctt acatgatttt
    14281 tcacataaat ctatctgaga aattccatct tgctcccata gaaaccttaa taacgcataa
    14341 tggtcagttg ttatatcata tccattttgt ttaaacatct tttttaaatc atcttttact
    14401 tttaacgaag tacttactaa cttagttgta gcttcatata gtttctcaaa atctttcatt
    14461 ttacctccat ttaaataatc ccttgctcct cccatctatc aattattccc ttatattgct
    14521 caatatcttt aaaagccatt ggctttaaaa cagcatttat agcaataaaa ttatagattt
    14581 gagaaagcac aggtggattt ttcttttcct ctatgcaata aataaatttt ttagatacct
    14641 tttgtatctt tgattctact ttttcagtta taggattttg attaaatcca gtttttatag
    14701 ataaactacc acaaacataa cctcccgaat ctaagaccat ggattttata aaagaaattg
    14761 cttttgaacc acgtgcattt tcatatgtag taactgctat acatggctta cgatacaata
    14821 atctttcaag catcatatgt gctctatctg taaacacctt gaaaagtcca gttacactac
    14881 ttccataagt tgggctacca aatataacac catcactctt agtaattata tcatgaatat
    14941 actctacttt atcattttga ttgatacact tgcccatttt ataacaattt aaacaaccaa
    15001 tacaatatga tggattgacc tcacttaaat caaaataatt aatttttaca tctggtatta
    15061 atctttctat atctttatac aagtaagtca aaacttttga tgtagctcca ttttttctag
    15121 ggcttccatt tataactgta attatcattt ctatatctcc tttttagttg ttatatcaac
    15181 aataagtata tcaactatat ttttagctgt caatataaac catttattta aagctatact
    15241 tacacagctt aaataaaagt tttatttaac tacatgtgct tatttaatca actataaatg
    15301 aattattttt aataattgac aaactaaatt aatgataata tactttatgt attacaaatg
    15361 taagtagaag gaggtatagt atgaaaaagt taccacatat ttcagaagca gaatatcaag
    15421 ttatgaaaat tatttggaaa tatgctccta tcagcactac tgaagtaatt gaaaagttgg
    15481 tagaaacaag tacatggagt cctaaaacaa ttcaaacaat gttattgaga ttagttaaaa
    15541 aaggagcttt aacatatgaa aaaaatagca gagtattcgt ttatactcca cttgttaaag
    15601 aagaagaata cgtagctaca gaaagtagtt cttttttaaa tcgtttttat aatggtactc
    15661 ttaattctat ggtactaaac tttttagaaa atgataaact atctgaagat gatattgagg
    15721 aactgagaga aatcttaaat aaaaggacga ctaaggggga taaataaata tgtatgcccc
    15781 attctttata cgatttttac tcagtacatt aaccttatca atattaattg ttgtaatttt
    15841 acttgccaaa aaagtattta aaaaacatat ttctgcaaag taccaataca atattggatt
    15901 tatattgctt ataatgttaa taattccatt tgtccctctt aaatatatta atcttggaaa
    15961 tgtatttaac tatttatata cttttaatca acttggaagc agtactaata ggacttctat
    16021 gataaaaaat agttcaaatt ctgtaattaa taactcaaat gcattaaacg atttttcagt
    16081 atccctaaat caagttaatt ttgatagttt aaatatttta ttactaatat catggataat
    16141 tggaatttta atcatgatag ctattactat tcattgcaac attaaaataa aaaatatgaa
    16201 aaaatcaata caaattttaa aagatgagga gatagtattt atatttgaaa agtgtaaaaa
    16261 agatattggt ataaataagg attttatact atgcaaatca cctattataa atacacctat
    16321 tacatttgga tactttaaac cacacattat attaccagat aagtctatat ctaaattatc
    16381 tttaaaagat ataaaatata tcttacttca tgaattacaa cattttaaaa ataaagatat
    16441 actgataaat tatataatgt gctttcttca aatattatat tggtttaatc ctcttgtatg
    16501 gtatgcattt aaagaaatgc gtatagaccg tgaaattgca tgtgatattt cagttttaaa
    16561 aaagcttaat aaagatagtc atatagaata tggacaaact ctaataaact ttgtggataa
    16621 tatttcaaga ccatcaaatc ttacacttac atcagatatt ggtggttcaa aaaaacaaat
    16681 aaaaaagcga ctattgaaga taatggattt tcataatgaa tcaaatacgt taaaattcaa
    16741 aagcatttta atttttacat tgatagcttt aattgtattt ggaaatattc ccaaactatc
    16801 tattatggca gatatcaacg aaaaatatag acctacaaat gaacaaactg tgtacgagga
    16861 cttaagtcag tattttaaag gttttgatgg aagttttgtt atgtatgatt taaaatccaa
    16921 gcaatatcat atttacaata aagaaaaaag taaaacaaga atttctccaa attctactta
    16981 taagatttat agtgccttat tgggattgga aaatgggata ataacaagaa ataattcaca
    17041 tatcccttgg gatggaaagc atcattctaa tagttcttgg aatgaagacc aagacttatt
    17101 ttctgctatg gaaaactccg taaactggta ctttcagact ctagataaaa aaacagggct
    17161 aaaaaacata caaacttatc ttaggcaaat tggatatggc aattatgatg tttcaggtgg
    17221 tatctcaaac ttttggctag aatcttcatt aaaaatatca cctattgaac aggtagaatt
    17281 acttgaaagt ttttatacaa ataagtttaa atttgaagat aaaaatatag aaacagtaaa
    17341 agattcattg ttaatatcaa aaaaggatgg ggtatcactt tttggtaaaa caggcactgc
    17401 caatataaat ggaaaagatg taaatggttg gtttataggt tatgtggaaa aaaatgaaaa
    17461 tacttatttc tttgctacaa atattcaaag caaagattat tctaatggta gttcagcatc
    17521 taagataaca ttatccatat taaatgacaa aaaaatctat aatccttaat atatttactg
    17581 tacattctaa aaaattcaca taattttcat attaattatc aattaaattc atatacaagg
    17641 cttattatat aaacatagaa gatagtaaaa ctagcttaaa aacataatat aataccctat
    17701 acaaatagtg gcaactctcc ctcacatcct caattgttgc cactatttaa ttttttgtat
    17761 gaagttaaat atattttcat catttcaaca atttgggtgc aatacaatct ctattttgaa
    17821 acaacctctt taattgtttt agagtttatg aattatttct catactttaa aaagattgaa
    17881 ttactcttga taatcttctt ttgtagtcat tacatatcac ctactactca acacatattc
    17941 tttgacctat ttttacatcc ttgtttatat ccaaaatatt taccttctta ccatctggaa
    18001 acaataccat tgtcaaagta gactttccta attcatgtat tctctttaaa tcaacattaa
    18061 caagaacgtc tccctgttta accatatctc cttgtttaac agcaacatca aagccatctc
    18121 catttaatgc aactgtatca attccaacat gaattaatac ttctccatta gttcctttaa
    18181 tcccaaatgc atgtccactt gaaaccactg tttctaattt acctgaaata ggagaaacaa
    18241 tttttccatc tataatatca atagcaaaac catcacctaa cattttttta gaaaatgttt
    18301 catctggtac attttctaat gcaacaattt tccccttagc tggagacaca aagttttcat
    18361 tattttcatt aaacactaat tcttcgtctt catcttcatt tatagcttct ttaggcactc
    18421 caaataaaca tgttacaata aagcagaatg caaccacaaa tattgatact aataagtatg
    18481 tgattaaatt atatccatca tagatataca ttaaataaga aggtaaaact gctaatccat
    18541 atgaattaga ttgaatatgt aaaattgata atattgctgc tcctaaacct gaagatgcac
    18601 acataataat caaaggcttt aaattatatc gaagctgaat cccaaataaa acaggctcac
    18661 ttataccaaa aagttgagat agcattgctc caaccgcagt ttgtttaact tgattatttt
    18721 tagatttttt cataaatgct agacatgttc caatattcgc aaatccatac attgcacaaa
    18781 gagtaataag ggcattaaat cctgtatttg ctaatagtga tgtttcaatc attacataag
    18841 tatgatgtaa gccagtaata acagctaatg gatatgtaaa tccaataata aatcctccaa
    18901 ttcctaaagg caaatcaatc aataagctga tgatatcaac catttttaat tctattgtat
    18961 gcattatagg gcctacacct aacaaaataa ctaacattgt tattagcata acaaaaaatg
    19021 gtgtgaaaat aaggtctaat acattaggca tatgtttacg gaaccatttt tcaagattag
    19081 ctccaataaa tgctgtaaca atagctgtta aaacacttcc ttgacaacct acaataggaa
    19141 taaatccaaa tgccataatt gcttcaattt catttgatgg gtcagcaaca gaatatgcat
    19201 taggaagtat tggggaaact aacataagtc caataacaag tccaataaca ggtgttcctc
    19261 caaatacttt aaatgctgac catacaataa ttgctggtaa aaaagcaaat gctgtttctg
    19321 taagaaccga aacaagtgtc tgaatataca atggaatatt tgcagaagac attccaaata
    19381 atccaagtac attatcatta aacaaacaac cctttaatcc caagaataat cctgttgctg
    19441 caataactgg aataatagga acaaatatat ctgctagagt tctcataact ttcttaaatc
    19501 cagtttcatt atttttaact aattcatctt gttctttttt tgataaagtt tttaatccca
    19561 ttttttcaac ttctgcataa actttattaa ctattcctgt tcctaaaata atttgatact
    19621 gtccagaagt aaagaaaacc cctttaacct catcaacttt ttcaactttc ttatcattaa
    19681 taatttctct atctttaacc atcactctta atcttgtggc acaatgtgta atagaaataa
    19741 tattatcttc accaataatt ttaataagct catttgctat tttattatat ttattcattt
    19801 ttctacctca ctttcaataa tactgcatga aatggtttca aacgcactgt tttatttaat
    19861 agatttatat catctaaatt ttggaataca atatcatatt tttcttctac tcttatttct
    19921 acaggtttat cagaaaaatt ataataacaa tcaataacta ccaaatctaa ttgccttcta
    19981 tacgcaataa tatcatcact ttctatatca ataggttcta ttgaaccata aatcaaacaa
    20041 tcagaatact cactatcttg tctcaacttt atcatctttt tataaaattc taaaatactc
    20101 tctgaattgt taatctgact ctgtgcatta atatctttat aattttcaat aacctttaac
    20161 catggtgttg tatctgaaaa acctgcattt tctttatctg cccatggaaa tggcgttctt
    20221 gcattatctc ggcttcttaa attcacaaat tctaacgctt cttcttctga gtaactttca
    20281 tctattgaac gatagtattg gtcaatgcta gataaatcat taaaatcatt tatggtatct
    20341 cttttaaaat ttgtcattcc taattcctgt ccctgataaa taaatggggt tcctcttaaa
    20401 aagaaataca ttgctcccaa catttttact gcatcatcat tcctttcgta ttgcaaaaga
    20461 tactttgttg tagctcttgg ttggtcatga ttttcaataa aattagctgc ccatccatgt
    20521 tcctgaacat acatttgact atctaaaatg cattttctta aatctggaat tgtccatgaa
    20581 attcttttaa accactcact accagaagca acatctaagt cagcatgttt aaagtcaaag
    20641 accattgaaa agaaaccatc ttctccaata aaatcaccta gctggtcata gctaatacct
    20701 gctgcttcag caacagtcat acaatcatat tttttaaacg tattttcttt taattcataa
    20761 agcatttttt ctattccagg ctgatttcta actgtttttg tacatttaac aagaccatca
    20821 acaccatcac tttctaaact ttgaaaactt aaattctttt tgatgaatgt aatagcatca
    20881 actctaaatc ctgccactcc catttgagac caggtattaa tcatttggta aagttcactt
    20941 cttaatattg gattttccca attaaggtca ggttgttttt taccaaaaga atgaaaataa
    21001 tattcgtctc tattctctac cttttcccaa acacttccac caaaaacaga cctccagtta
    21061 tttggtaaat cgtttccttt tttaaaaata taataatcat gataaggact ttctggattt
    21121 tttaatgctt cttgaaacca aggatgttca tcacttgtat gattaagaac taaatctaat
    21181 ataattttga ttcctctatc tttactttct ttaattaact cttttaaatc ttccatactt
    21241 ccaaattcac ttgcaatatc caaataatca ctaatatcat acccattatc gtccataggt
    21301 gatttaaaaa ttggacataa ccacaatgat gtaataccta aattttttaa ataatctaaa
    21361 cgagaaataa ttccctgtaa atctccaatt ccatcattat tactatcttg aaagcttctt
    21421 ggataaattt gataaacgat ttctttttgc caccatttct tttccataaa ttactccttt
    21481 ttatatgata tttcttcttt ttaattcttt ataaattcca ttatcgaata tgtcctcaca
    21541 tatagatgtc gctatatctt taagttgttg atgactattt ttcattgcaa ttgtaacgtc
    21601 tgatgcttga aacatgacaa catcgttttg accatcacca aaacatatgg tttctttttg
    21661 agttactcct aatctttctt gtaatctctt aattgctttg cccttatgaa aatctttttg
    21721 aataatttca taatactgac tagaaatatc tctctgtgct aattccattt tatcttgtag
    21781 tatatttttc acttcgtcaa ataccttttc atttgaccat aaacaaattt tatgtatatc
    21841 ttgtgactta tactcttcaa tgttattttc atatgttatt ttttcttgga tatgttgttt
    21901 atttatacaa gaattagttc ctttcaactg attcattgtt tcaaatattt ctttggcttt
    21961 ttgattcatg aaaacttttt cctgactctc aatcgaaaaa gcaacttctc tcttttttaa
    22021 taaacaaaca acttccttta tcaatctttg attaaaagat tgattataaa gtaattcacc
    22081 atgatattga atgtaattac ctcctcctgc aatatatcca tcaactccta atgataaaac
    22141 atcatcttgt attgtaccca tacttcttcc agtacaaata acaacactgc aatgattctt
    22201 ttgacataaa cgtatagcat gctttgcaga ttctgggatt ccatatacct catctctaag
    22261 cgtaccatct atatcaagta ttaataattt gtacaatttc atcttccttt cttactatca
    22321 aaatcataat agcattcaaa aaaaaagata tcaatatttt gatatctttt aaaaaatatg
    22381 ttcattttta gcacaaattt tcattttcgg ggaatatgtt cactttctaa atacatcttg
    22441 tatttaatat aaagcagttc aattaaaata aaataaggtg ttgttacatc gtagtccagt
    22501 tgtttatcta cagaaataga tatagattga atatacaggt tttcatcaca taatcttgct
    22561 aaaggattac ttatcatttt tgtaatagaa accatataaa ttcctttcat tctcatttct
    22621 ttagcaaaat caataattgt agatgcatct ccattcaatg aaataaagaa aatcatatca
    22681 tctgttttta taaaatgatt aagtgcctga atcatatcat aaccataaac atcatatata
    22741 attttccctg ttggtaagaa aatccttttc atctctttag caactctttc ctgagaataa
    22801 ccttctccaa agacgattat acgatttgcc tttttcattc tttcaaacat aatcgaacaa
    22861 tccttcttct ctatatcatc tatcacctta ttataacaat ccattaattg gtgtttactt
    22921 tgatttttat attctacttc tgtttcattt aagcgtataa taactcttaa ttcactatat
    22981 ccaggtaatt gtagtttttg agcaaaacga gaaagtgcac ttgtagatat atggtatttg
    23041 tctgcaaatt catctataga aagtttaata caatcttctt tatgattaag aatacactca
    23101 cagatatact tatcatttgc tgtaaatgaa ttataatatt tattgaaaag tccttcaatt
    23161 ttcattcttt tctccttcta tactctatgt aattcatatg aagcaaatct aatagagcat
    23221 aaaatgctcc tacgacttca taagatatag cttgtaatgt atcaagtttt tgcgtttcaa
    23281 catacagatt atactcacaa agtgttgcaa ttgtattatt atcaagccta gttaaagaaa
    23341 tcgtatgtat atgattgttg ataaattttg caatttcaat tcctgctttt gtttctccag
    23401 acaaagaaat gataataaat aaatcatttt tttgaaatga ttctctcata aattcaattt
    23461 cacctatatc aaataagtca ataacacatt tacctactga aagaaatatt ctctttattt
    23521 cttgagctaa acttttctgt tcattacctg tcccataaat ataaattgtt tctgcatcat
    23581 atattatttt acaaatatct tgaaatgata ttttttgtaa ttcattcatt aatagtcgat
    23641 aattctcgca aacatcatga aaattattat cattattaac aaattctagt tgattatttt
    23701 taagtaaata ctttaaatca gagaatgaat ctaatccaat ttttctacaa agccttaata
    23761 aagtagctct agaaacatta cattcatttg caattttttc acacgaaaaa ttacggtatt
    23821 ctctttgaca aatcaatatt tgtttaataa taattttatc attttcattt aaattatcat
    23881 aattttggtt aactaacgat tctaatctca ttttttccac tccctaaata ttattttaat
    23941 atacattagt tgctttttct agggaagagt tttaaataat aaggataaaa taatcatttg
    24001 attttatttt tattaaaata aaaataacct gctaaaagca ggcaccaaaa agtacttatc
    24061 ctttcgaaca tgtactttag atattgcttt cttcggaagc ttcatctctt catactctaa
    24121 aatatagttt tttctaactc aaaagtattt tccatggtag aaattacttt aattcatctg
    24181 ccatttactt tttatcttat ttaataatta ctattacttg aatatttact tctttaagta
    24241 aatttcatag ttaacaaagc taaaactatt attatagatg ttaaaataca aaattgtaag
    24301 tattgttata aatatgctta cagctacaaa tatacatgat atattaaaaa gaagctgtaa
    24361 attattatca tacatacaag caactatgcc tctattaatc catgttaatt tttattattt
    24421 aatacaagga gttccatgag acatacatat ccattcatat tttaaattat ccaattctct
    24481 tatagatttc accatctttt tataatccca attccatgga ttaggataag gaactatatt
    24541 tccattctta tttttgacta aatcacctgc aaataataca gcttcaaaaa tcatgcagac
    24601 atgccctgga gtatgaccag gagtatggat aatttttata ttcccaatct gcatattctc
    24661 tccataaggt tcaacatcct taatgagcct tctggaaatt gcctttccaa taaacttctt
    24721 aaacccaggt ctatccttct ctcctataat aaaaggtata tcctctacat gagcccatac
    24781 ttttgctcct gttttttctt tcaataattt aatattagat atatggtcaa catcatgatg
    24841 agttaataaa atatgcttaa tatctgtaag tttaatgcca agtgttttta attctttaat
    24901 tatatttttc cccataaaag gaagacctgt gtctataaga ataatttctt tatcaaacac
    24961 aataaaacaa tttgagccac gagtactatc taacattaat acattttctt ttatcttcat
    25021 aaataaattc cccttatttg gtattttacc tatataatta attaataaag ttataaatag
    25081 ttataatact tgatattaat tttatttttt attaaagtat actataggta tacaagtatt
    25141 atatttacat aagtatacca tcagtatact tttttatcaa tagaaaggaa taatttatat
    25201 gtcagaatta tcaaaaagag ataaagaaaa aatacaaaga gaaaatgaga ttattgataa
    25261 agcagaaaaa ttattttgtc ttaatggatt tgataataca actatgaatg aattagcaaa
    25321 agaagttgaa tatactaaac gcaccatata caagtacttt tcatgtaaag aagatttatt
    25381 ctttgctgtt gtcttaagag gatataaaag actttgggat aatgttaaaa tagaaagtgc
    25441 aaaaggcaaa acagggtttg aaaaaataaa actttcatac tttgcttttc ataaatttta
    25501 ctctgtagaa ccttctctat tatgccttat gggaatgatt ggtattgtaa actctgaaaa
    25561 atcggatact gatatgctgt ttaaagaaaa gtttttttct tttaataaat ttatgtttga
    25621 tgaaatacaa ggaatgtttg aaattggaaa aaatgataga agtattaggc atgatattga
    25681 aatacctact cttatgtatt catcaatatt tacacttaca ggatttttta atttactatc
    25741 tgtaacagga aaatcatacc tgaacaactt taatatagat gaggaaaaat ttattgaaac
    25801 aactcttgct cttttaatag attcaattaa agcataaacc taagtttata aattaaagtt
    25861 tatatactta aatgataatt ttttctatct ctaatataaa aaatattaaa cataaaacta
    25921 ataacttagt taatggaata actaaattac tgaaaggatt aaaagtaaat ggctaaaata
    25981 cttcttttag aagatgatga tacaattaca tttggaataa aaacttccct tgaaaagaaa
    26041 aatcacactg tatgttgtta taatacgatt gctgaagcta aaaaaaattt tgataaaact
    26101 attgatttaa ttctgcttga tttaaatcta cctgatggta ttggatacga gttttgccaa
    26161 tatgttaaaa gtatagaaga tactccagtg atatttttga cagtccgtga tgatgaaaaa
    26221 gacattatac gtggattaga tatgggtgca gatgactatg tggttaaacc gttttttctc
    26281 tcagtacttc agtccagaat taatgcagtt cttagaagaa ctcattcaaa tactaacaat
    26341 actattacat gtgaaaatat aagcttgaat aaggttgaag tcaaagcata tttagataat
    26401 gttgaagtac ctttgacagc aggagaatat aaacttcttc taagtcttat ggaaaataaa
    26461 aatagagcta tgactcgtac aaagttacta gaaatgctct gggatgtaga tggtaatttc
    26521 gtaaatgata acactcttac tgttacaata aaacgtctta gagaaaaatt aaaaaatcca
    26581 ccttgtataa aaacgttaag aggcatagga tatagaatgg aggacagcta gtgaaaactc
    26641 caagaaatat caacagtaca gttgttttta taatattctg cttaattgta acttttttat
    26701 tgagtgtaat tctaacaact ataacttctt ttaatgaata caatgtactg tctaaggctc
    26761 ttggtgcatc atatgagtac aatcctaagt ctacagaaag tatactaaaa tcactgaaaa
    26821 attctagtga taaggatttt aaaacaggaa atgaaatttt atcaaaacat ggatattcta
    26881 aaagtacatt ttggagtaaa aacttttttt attttctagt gatatcgttt ggtataattt
    26941 caacaatggc tacagttata tatataatca aacatagaac aaataagcaa aaactgagta
    27001 gaataaacaa tcttactcaa tacttagaag aagtgaattt aggaaaagag tctgtacttt
    27061 tacgatgtga agatgaattt tctcaccttg aagatgagat atataaaaca gttggggaac
    27121 ttcgttgttc aaaagaattt gctttaaaag aaagacaaac tctttcagat aatcttgcag
    27181 atattgctca tcaacttaag actccaatta cttctatgtc acttatggca caattacttt
    27241 cagaaaattg tccagatgaa gaaatagatt atattaacag acttacaaat caaatctcaa
    27301 gacttgagag actagtttca tctttactta cattatcaaa attggatgca agtactttaa
    27361 tatttgaaag caaacttata gatgtacatt ctcttcttac ttatgcaatt gagcctatag
    27421 aaggttcttt aagagaaaaa catcagacat ttactatcga ggcttctcct tcggtagaat
    27481 ttgaaatcga tattaattgg actttagaag ctcttttaaa tattcttaaa aattgtagtg
    27541 agcatataga agatggtgga tatatagttg cttattatag tcaaaatcct ctatatgttg
    27601 agattatcat agaagataat ggtaaggggt ttgctcagga agaacttcca tatattttta
    27661 atagatttta tcgtggtaaa aatgctagta aggacagtat tggtataggg cttgctctat
    27721 caaagtctat tatagaacgt caaaatggta ctctacatgc agaaaattgc cataatggtg
    27781 gtgcaagatt cataattaaa ttttacacat aaaatgctgg attataggta aaatacctat
    27841 aatccagcta tatctatatt ctatgttatc tattgtttta gtaatatact tctatttaca
    27901 tttaaggttt tattaaacaa acaacttaac tatccctata cttgtaagta gtcttaaatc
    27961 gaaaccttta aataagttca aatagatttt gtttatattg tttcattttt tatagattct
    28021 actatatttt cattcttcaa ctgcttgtat cctgtcatat atgatgctat tacaataaaa
    28081 agtatcaata tagtaaaacc tattattgta gatataggtg caaatggtaa atactctata
    28141 aaatatattt catttatacg tagaaaagca tacactattc ctatttgcac aggaatacta
    28201 agtagtaatg gtgtaattcc taagaataaa ccttccaaaa taagcatctt ttttatgcct
    28261 tctggagata atccaacaga acgcaacata gcaaactctt gtcgtctact tctaatattt
    28321 cctgacactg tagcaagtac atttgataat ccaatcatag ccaataaacc tgtaataaag
    28381 cctactatta aattcatggt ctttattcca ctagcctttt gtttttcact ttctaaaaca
    28441 tcctcaatac tataatctcc tgaaccatag tctttattat aaatggactc taatttttct
    28501 cttactctag gaatgtcctc acgattattt actttaaata ctcctatcag cctatttgaa
    28561 cgtaacctac ttacttcatc ataattttta gatatttcct gtaccataga cattggaact
    28621 atttgcatta atgtatactt tgaactagta tctactattt taggaagtgt gcttgtaata
    28681 gctcccactt tcatgttgta agtataatcc cctttatctt cgtcatatac tttttcattt
    28741 aatttaatgt tatctcctat tgaaagattt aaataatcta tatatacttt atttctacgt
    28801 gtactacgat taatatcttc ctccttatta tatacaattg aggttggatt ttttgtatta
    28861 taaaataact ttgggtctac agaaatttgc ctacaatatt ctgtaaaact cttatcatca
    28921 agcccaatta tatttgaaat tatacgaaac ttgccatcac gttctatagg agagtatttt
    28981 ttagatgaaa ctatactttt aaatcctcca tttttcgcaa gttcttttga tgcatctttt
    29041 tcagtcagcc aagtggaagc agacattttt tgataatata gtgaactttt tacaccttct
    29101 gtattatcta atttttcaat gaaagcagac tctataggtt cactattttc caaataaact
    29161 gctatatctc tttggtcctt ataattatca cttccataaa cagcttttgc tccttcttga
    29221 tttgcaatta tatgcaaaaa acatgtaagt aataaaaaag ataatgttaa agatattgtt
    29281 gcagtacgat aagatttttt acgagcacgt agtgcgtttg atgcaagttc tccttctatt
    29341 ccaaatatct ttcctaatga atatttatgc gactttttga ttttaacact atctccttgt
    29401 cgtattgctt ctataggaga tatctttgat acttttcgtg ctggtataag tgcagaaatc
    29461 cagactgtta ctattgtaag taaaactgct ggcagaaaag caggaatacc ataagtaaat
    29521 accacttctg gaacatcagt atgataatta actgaattaa cataaactat aagcctatta
    29581 cacaataacc atcctaaaaa taaacctatt ggtaatggta taattgtcag taaaaagcct
    29641 tcaaacacta cagacctttt aatttgtttt ggtgatgcac caacactaga taatatccct
    29701 aactgagtaa gtctagcatt tgcagacagt gaaaacgcgt tgtaaatcac cattacaaac
    29761 acggctactg taaaaattgc tattgtaaga tacataatag gagttgaaaa ttttgatata
    29821 ctactcatag atgctttctg ttctggtgaa aagactaaca tctttaaaag ataattacta
    29881 ttgtatttaa gattgtattt tccatattca ttagtcttcc atcccaatga tttagcaagt
    29941 tttggaagtt ccttatacgt gtctcttata tttttaaatc gcaagtacac agttatacta
    30001 tcattaggtt ttatacttgt tttatcaaga tatccaagag ctgtatatgc aggtattact
    30061 gatgaagtag aaacatctac ctttcctact attgtcaatg tagcctctga aattttttta
    30121 aaactttccc ctttcgattt tggggctatg ggttcaagag gctttccatt taagattcta
    30181 tatcctattg ggagagtaat tttatcacca atttttacac ttggattatt ctcaaaatat
    30241 tgttttgata atgcaatttc attagcatta tttggtcgac gaccctctat tatagtatcc
    30301 ttttctggca tagaattcca atactctgat gtagcatttc tataaattat ataatctctt
    30361 ttctcatcat taattttgcc tacataccat tgtcctttta ccattacatc atcaactgaa
    30421 gaaaaatttt caattaatgg taagtcactt ccataagtat agtcaaacaa ttcaccatgc
    30481 cagttacctt ctttctcaat tgaaagtctt ataaaatctg tccacatggt atacaacagt
    30541 ccacacaaag aagacatcat ggttgtagta agtaatatag ctaacattat agctatacta
    30601 ctacgtttat tgcgtcgtac aaagtctaag gtgtattctt gaagaatatt catttcttca
    30661 ccaccttttc agagacaatc actccatctt ccatagttac aatacgattt gcctctagtg
    30721 ctattttctc atcatgagta ataagaagta ttgtctgatt aaatcgttta tttgataagt
    30781 ttaacaagtc tacaatttct tctgaatttt ttctatcaag attaccagtt ggctcatctg
    30841 ctaataaaat agctggtcta tagattaaac ttctagctat agataccctt tgttgttgtc
    30901 cacctgaaag ttgacttgga agatggttta atctatcaga taatcccaat atagatacaa
    30961 tctcagagaa tctatcctca tccacctttc ttttatcaag taacatgggt aataatatat
    31021 tttttctaac atcaagggta ggtataagat tataaaattg ataaataaga cctacttttc
    31081 tacgtctaaa aactgcaaga gcttcttcct ttaaacttga tatttctgta tcttcaacat
    31141 aaatctttcc agatgtaggt ctatctactc ccccaagcag atgtaacaat gtagatttgc
    31201 cagaaccaga tgctcctaca atggatacaa aatcaccttt ttgaactgat aaatttacat
    31261 tattaagggc tgtcactctg gtttgatttg aaccatatat ttttgtgaga ttttcacatt
    31321 ttaatatttc catatacaat catctccttt atggttaaat tttagcaacc taaaatgaca
    31381 ttatagtgac atgaatacaa atataaatta ctattaaaaa tattgtttag tttttgcaca
    31441 ttatttaaaa ttataatata aaacaaaaac tatgcaatat attttattta tacttttaat
    31501 atattacata gtctttaaaa ggtattaact ttaaatctat aggtattcaa tttaataatc
    31561 aatagcaagt attttatgta ctaaaggagt tttgctctat atagactcaa tttagtaaaa
    31621 ttatttaata aatattacag actataggta aataatattt acaaataaaa ttaagtttat
    31681 tcccttacac tataatattt cttaattata ttaattgcaa tttcctttac ctttggccct
    31741 gtaatttctt tattggaatc atctgattta atagcaaaat aataggtgtc accttcacgc
    31801 tctagcatac caacaaacca tgcatccaca cgtttatttt tttcatcaaa tcctgttcca
    31861 gtttttccat atacatttat gtttttatca tttatatcta ttttcataat atctcttaaa
    31921 atattaatat gctctttttt aaagtttgta tctccctcaa atatttttgc cattgtttgc
    31981 acttgttctt ttggagatat ttgtaatgaa gattctaacc aaaatccatt taaatgtccc
    32041 tttccatttt ttaaatcgcc ttcccattcg ctaatatcgc aattcccata ctttaaatca
    32101 tctaaaatgt tttggacaga tttagcatca actttatcta tcaatttttt gtaataccaa
    32161 acacaagact ccttaaatgc ctcttctaaa ctaagatttt tattccaatt tttatttgga
    32221 tactctgtac catcatatcc cataactgat tcttttgagt ttattacccc tttttctaaa
    32281 ccaattaaag ttgaaactat tttaaaagta gaacatggtg accttcttgt ttcaattaat
    32341 tctttgttat atatattata ttctttattt ttagtattat aaaaaatagc tcctccacta
    32401 ataccttcaa aacaatcact atagtcaacc atattaattt tttctttttt gtttttatca
    32461 ttgttttcaa ttttggattt atttaattca gacttattta attcagattt tatataattc
    32521 ttatcagttt tttgatttat atcgttatgt tttttcccta cataatacat taccataact
    32581 acaccaacca ataagatagc tatccaaata aaattctttt ttcttttcat aatttatatc
    32641 atctcccata tttaaattta taacctacta ttaaaatagt agacattatt tgtaatatac
    32701 tccaatctag gttctactta taagaaacaa gactaaatga atagctgtat ctcttatatt
    32761 ttcttcacct agtacaatta tgttgattaa acctattgcg aagataatta ggtaactatt
    32821 aataacatcc tgtcctcctt ttaatttgtg atataataat tgattccaca tttaaaactt
    32881 actattgtaa tatttaaata ttacaatagt aagttttaaa tgtcaagtta gtttaggaaa
    32941 gttattttaa ttgtataatt gttattacat ttataaaata tggcatgttt aaaattattt
    33001 tgctattggg aggattaaga taagcagtat tataaaagct ataaattata aaatttaata
    33061 tataatttat agcttttact tttacaaata ttgtttttat tgatttatta atgaatttta
    33121 ttgacaggtt atatttatat aagtaaacac ttacataata agtatataat ctacatatct
    33181 ataagttgag tcaattcaca agtatatgtc aacttaagtt tatgcatagc tcgtgtacag
    33241 gcaatatata acaaactgcg gtcataatca ctataatatg ttttattatt tactgaaggt
    33301 acaataactt catcaaactc taaacctttt gacattttaa tagaagttat ggatactcct
    33361 tttgtaaagc tagaactttc agaagatatt aagtgaacac tatattcttc tcttaacgca
    33421 tcatatacag ctttagcgtc attatctgtc tttaatataa taccaagagt cgcattatca
    33481 ctctttttaa attcttcaat ttctattttt atcttattta tttcatcttg ctcgttatta
    33541 cattttaata aagctggttc ttcaccatgt cgttcaatag gctctaatga agatatgtct
    33601 tgaatctttt tggcgaaatt aataatctca aaagtagaac ggtaactctt gttcaacata
    33661 actaattccc catcattata tagctgtttc atatcatcta gtgtatgagt atgattagga
    33721 ttaaccaatt gtccaaaatc tccaagaata gtctttttac acttaaaaag taagtttatt
    33781 acagcatatt gaataggtgt atagtcctgc atttcatcaa taacaatatg ccttataatc
    33841 ttatcttctt gtataccttc atatgcagca tgaatatata taaatgggta tacatctgac
    33901 cattcaagtg ttttcttttc tgccatcaca aacatgtttg atatattcat ttgtttaaag
    33961 aaatccttat atagtgtaag agtatttttt attgtcaaca ttccattcaa acttttaagt
    34021 atacttttta cctttggtaa atcttcttcc ataatattat ctgattcaaa tttataatga
    34081 atgtcttcag ctaccttctc taatctcttc ttaactggat atctgttata tgcctcaaat
    34141 ctactctgta tccagtcact tttagctgta aaacttccaa aagtgtagtc ttttggaata
    34201 aaaatcttat ttggcatttg ttttatataa tcatcaataa gttttacaaa atcaagtgta
    34261 gatttaaaac gcactctttc agaccattta gcatcattta tttctaatgg gtctttctca
    34321 ctttcaaaat taataactct atctaattgt acctctgcta tattttcaaa acttaattca
    34381 cataatggct cttctccaag ctcaggaagg acattggaaa tatagtctcc aaagacttta
    34441 ttaggtgata ggataattac attatttgct gatattttat ctttaaatcg ataaagaaga
    34501 aaagcaattc tgtgtaatgc tatagatgtt ttcccagaac cagctacccc ttgaataatc
    34561 agtgtatcag ctttatcatt tctaataatt tgattttgtt ccttttgtat tgttgaaatg
    34621 attgacttca ttttttcatc tgaagtgtga ctaagttctt tttgaagaat atcatcttgt
    34681 atattaatgg aactttctag tgcatattct aattttccat ttttaatttt aaattgtcgt
    34741 tttcttgtta tttctccatc cacccatcct attggagcat catatccagc ttttccgatt
    34801 tcataatcat aaaacatact ggcaattgga gaacgccagt caagtattat aagctcatct
    34861 tcataatcaa atgcaaatct accaatataa tacttagatt catcatccat atctttcaac
    34921 ctaaaatcta ttctagcaaa gtatggagaa tctttgattt tattaatctt atctcgaact
    34981 tttaccataa aagccccata gttatctatt tgttttagtc cttgttcatt ttgaaacatt
    35041 tcatgtgggt ctatttcacc acgatttttt accatataca ttttagcatc catataatcc
    35101 ttatcaagct tgtcaacaag attttcagct ttcttaaaag cattgtctag cttgttattt
    35161 atctctatta gatgtgcaat ctcatctgga aaaaaagtta ataactctgc atcatattca
    35221 cacatattat gtttagaatt agatgcaata atatcgcaat ttactacttg tttattttca
    35281 gacatttaaa tcctcctttg ttaattaagt acttacactg aatcattata cttaattgtg
    35341 ttttggagtt tttaatcatt cttgctattt aacttaaagg aagaataaat ttttgagtag
    35401 gatatccaaa ttccacaagc aattctgctt ctgcaaagcc caacttcttt atcatatctc
    35461 tatgtcctgt atcagctttg tcaccctctc gaaaggtagt aattgaaata tctgtatcta
    35521 tcaagaaatc ttcaataact ttcttcaaaa acacttgtgc aatgtctgaa ttacgatact
    35581 gtggatgtat tccaaaaaaa tcaatacttc tagtttctct attaaacacc atatttgcaa
    35641 tagcgacatt attaagtttt aatattaaag ctctttcttc taaaatatac tgttctaatt
    35701 gatatatata ttcttcctct tgcaaattgg gaaatccatc tatgactaat ctcactaaat
    35761 tcatccataa tggtatgtca gacatacttg caagtttaat ttctttttcc aactctagta
    35821 cattctttga aaataggtta tcttttttca aaacaaatct tagctgcaaa ggataaaatt
    35881 cttgttcttt cctatattta tttggagatt ttttatacat agctttaaat atagaagtga
    35941 atgcctgttg actttcatat ccagctatca atgcaatttc aataattggt ttctttgaaa
    36001 ataccaataa ttttgctgct tctgtcaact ttctccgctt tatgtaatca tgcattgtta
    36061 ggccaacaga agttgtgaaa gttctatgca ggtgatattt tgagtaatga acagcattag
    36121 caactatatc taaatccagc ttttctgata aatgctcctc tatatagtca atagcagtta
    36181 tgacattaat tactttgtaa gacattgcag cttccctcct tttatccatg caacattata
    36241 tcaattatta cttagttata tttaatgaat cttgctattt tataaacaaa tttgttgttt
    36301 aagttttcaa ttcataaatt taatatatta ctttttactt tctgattaaa gactctaatc
    36361 ctttaatttt caataacttt tcatttagta aatctaaaaa gttatttatt attttcttct
    36421 tattagattc cttaattaaa tgataaatat ttatattata cgactaaaaa cagcttttta
    36481 caattattta atatgattac aaacttaccc tatcttaaag tttaatgggg tcctgttatg
    36541 aatcttttta ttttaattta agctcttcat cagtttattt tatatattga tgtatttgat
    36601 ttcatacaaa aaagagtgct tcatgaacta aaaagttcat ttttaaacac tctcttttat
    36661 gtaaataaat ttctattcta ttttgaaata ttataatttg acttataatc tattaatacc
    36721 aaaaataata aattgtacgt ctaagaatag attctaatta gttgctcagt agtaaaatct
    36781 gtttttaact aatcctgctt tgtatgttga acatattcta taattgtacc atctggatgt
    36841 ctcaatgtca tattatatcc tgtaggaact tttttcttat ctcttactac aactgctcca
    36901 ttatccaata gataacttct ccattcatca acagaatcaa ccataaaagt agattttgtt
    36961 tctataaatg gtttaagagc atcatcagaa ccagataata gtaaaacatt tcctatttgc
    37021 gctaattcca acccaacttc tttataggaa aaacgcaaag aacatttttc ttttaaaaga
    37081 ttctcataaa aaggtatggc tttttctata tcttgaatat aaaaacgtgt taatatatca
    37141 tatattttca tattttcccc ctatatatct atataatatt acctctaaat tataccactc
    37201 tacaaattat ataacaataa attaagcata gccttttgag catcttaagt agaccttaac
    37261 aatatattgg ggttacatta gaatacataa attaaatcac cttgatatta tttaggataa
    37321 aatttatgta gtagcagtaa atgttcttct aattatcaac taacaaacga taatctttaa
    37381 ctttctatat aatgggtaac atcattttcg taagatattc actagctgga cgattgatat
    37441 catttaaata ctggtaatat atatcgccat gaagtttata tccattgcta tttgcccatg
    37501 aaaataagtc ttctaaagta gggtcttgta attcataaag tcctaaatca attgcagtta
    37561 ttattttctg tgcaggaaca atcttttgta gaatctcatt tttcccatct atataagtgg
    37621 ctacaggaaa ccctacttca acatccaatt ttactaaatc catgttatga aaacatacaa
    37681 tcggtgctcc tgaagataaa attcctctct cctccagata tttagaaatt ttttcaaaac
    37741 tttgttttga aaactctgaa aattctacca taaagtcaat tgtttttcgt attgccaaaa
    37801 aacagtactc agattgttca gctattgtta tattggatat tctagtcatt ttattcctcc
    37861 attactttat tttcatttct aatcttttta taccaaatta tacgaaaatt cactgtgtaa
    37921 ctaattattt catatgaata taaaacataa cctccttcca tttcttatat cgaaagtata
    37981 gcaaaaataa tctcatcatt cttgtcacac agtgctctta taatcagaac atacttaaaa
    38041 gacttgccta atttttaggc aagtctaaaa gaaatctttt tctataaaaa atttatacaa
    38101 acaattaaaa tatcaaaata tagactaata taaaactata aatttactaa tctgaacctc
    38161 ccatggctaa agtcacaaga ttactgattt atagaatttc gcacactaaa atatgtctta
    38221 ctaattcccc acaggctacc cccctagttc ctacgttctt acataagtta tctattatca
    38281 aacatcaaaa tttaaacaag tttcaggttc atcttctatt cttgctattt taaacttaca
    38341 tgcaattaat actaaagata ctattggatt tataatattt aataaagcgt atggaccata
    38401 tgttagtgca gatacaccta atgttgctgt tatatacgcc ccgcatgtat tccatggtac
    38461 taatggagaa gttaaagttc cactatcttc taaagctctc gacaacatct taggatgtat
    38521 acctctcttt ttatattcct tattatacat tcttcctggt ataactatag atagatattg
    38581 ttcacctgct attatattgg taattgtaca agttaccata gtacataata ctgttccaaa
    38641 tacacccttt gctaatctta gtaaagaaga tgctactact tcaagcatac ctgttttttc
    38701 taaaatacct ccaagagata aagcgcatat tgttaaggat acagtggaca tcatattcat
    38761 aatcccacct ttagataata aagcatctac ctcctgtatt tgtgttgacg aaacatatcc
    38821 attttgtgct gcatctaata tcgtattcat agattctcct tgaaaaacta ctgcaaacaa
    38881 tactcccaat acagcaccta ctataagccc tggtatagca ggaactttaa atacaaccaa
    38941 tccaattact attgctggag ctaataataa tactggtgat aaagtattaa atgtagaatc
    39001 taatgtagac ctaattaatt ctatttgttt tatatctagt gcttgacctg aatacttcat
    39061 tccaattaca ccataaatta ctaaacaaat taataatgat ggtatagtcg aatatagcat
    39121 atattttata tgtgtaaaca aatctgttcc tgccattgct ggagctaagt tagtcgtttc
    39181 agataaaggt gatagtttat ctccaaagta agctccagat attatagaac ctacgattaa
    39241 tccaattggc atacctaaac tttgaccaat tcctaaaaga gctactccta ctgtacccat
    39301 tgtgctccaa gatgaacctg tcgcaagaga tactattgca cagactaatg ttgatgctac
    39361 taaaaaaata tttggtgata aaatttttag tccccagtaa atcatacatg gaactactcc
    39421 acttactatc caagttccta taagtacgcc tattataatt atgattaaga ttgcttgcat
    39481 tgccatcttt atactattaa acatacttaa ttctaactct ttccatttaa atccaagtct
    39541 atatacagcc acaagtcctg ctactgctgc tgctcctatt agtggtacat gaggtgatga
    39601 accatacact aatactccta gacttaaaaa taatatcaaa caagtaattg gtatcattgc
    39661 atcaaacaga cttgcttttc tacattctcg ctctttcata aaatagtttc ctcctaatta
    39721 tttaaacact acactataat tttttttgaa acatatttgc tcgtactaga tgacatgata
    39781 cccccataag ttaataaaaa ttctaaattg tcacctaatt tataatcata ttttgtgtta
    39841 cttacatcta gtatcatgtg gtctgaactt gcccctaata tttttatatc agtgtctatt
    39901 ggaattaaag agtcaatatt tatatcttgt ttacctatag ctatgattgc cctttttaag
    39961 attcccttat cttcataaac aggtttattt ctaaaggcat caactcctat ttctccaata
    40021 ggaactgatg gtttttcttt acattcaact acttggcata ttaatttaaa tgcatcttga
    40081 tatgttccat ttatattttc accgtatgcc gtttctctac caagtagaat ggactcgcct
    40141 attctcaaat tagtaatacc ttctggcata ttattattta tcaacagatg aatagaactt
    40201 gaattacctc cagatactat ttctagattt attccaaatt tttcttccat tctttttgct
    40261 atacttacca gtctgcctat attttcccta gatggtataa tagctccata acaggttaag
    40321 tttgtagcta taccaattat ttcaatattt tttaatctta ctatctctct tacattatta
    40381 aaaaagtctt cttcataaaa gtatccttct cgtaaatcgc ctaagtcaaa cataagaact
    40441 atcttatgaa taatattttt actttcacat aattcattta atttctttat tgttcctaat
    40501 tctgagttaa aactaatatc gcaataattg ataacttctt ctaattcact taacattggg
    40561 attctaatca atatcttagg tagatttata tcttgtaaat tttttaagtt ttgtattctt
    40621 gaatctgcta tatggtctat tccttcacta catatttctt ctactatttc tttttgtgca
    40681 caaaatgatt tagtaacaaa agatatctta atatttcttt catgacacat ctgcgaaatt
    40741 aattttgcat tatgctttaa cttttctata tctatttcta acctaggata cataatcttt
    40801 cctcctcaaa attcaaactt tttaacagga gcttaagaga tgcttcttca tattttttag
    40861 ataaaacacc catagatgcc ataatatagt cattatctat taatatcttt gttttttcct
    40921 ttggaaatag taattcatta tttttattgt atttgagcat attatctaat atttcaattc
    40981 tactagggag cctagtaagt gctccccctg taccaactat atatcttact tctgttaaat
    41041 ctttcccttc tgcaattttg acttttccag ttgttccata gatatttctt atttttcctg
    41101 aatgtcttag catagcttta acaacagctt cttttgtcaa tctttcaaca aataatattt
    41161 cttcttttga ttttggtatt ggaggataat tctctattac tgcatcaata tctatagata
    41221 gttcactttg taagttttct ttacctataa cttctactat attcttcata tttacatata
    41281 cacctaaatc cccctcaaca cttctctttg cagtaggttc aggattaacc aaaattttat
    41341 taatatagtc acttccatct gtaacagaat gtacatcagt agttgctcca cctacatcca
    41401 gagttaataa atctccaata tttttatata aaagtttaga agcttccatt acagctcctg
    41461 gggttggagt aatattttca ttaaccattt ctttaatgta tttcattcct ggtgctgtag
    41521 ttatgtgttc ttcaaataca ctttgaatga ttcttcttgt aggttctata ttcaataaat
    41581 ctattttagg gtatacattt tcagttatat aaagcttata attagtatct tcaaatatta
    41641 accttatttc ctcttgattt tcaacattac cagcatatat aactggtatg cctagattca
    41701 tagaagctat catttctgca ttataaatag ctgtatctct ttctccataa tcgactccac
    41761 cagctattaa aattatgttt gggtttattt cttttatttt atttaaatca gttcttttaa
    41821 ttctacctga tgttatttga cgaataacag ctccagcccc taaagctgct tctttagctg
    41881 cttttactgt catatcatag actaatccat gaacagtcat ttttaatcca ccagctgcac
    41941 tagaagttgc aaacatatca ttatactcta tatcatctac agataattta ttagctaaat
    42001 ctttaatagc acctgataag ccatttctta catcgccacc ttcaaacact gttgttggag
    42061 cttgaccttg acccaaaaat ataggattgt tagtattaat atcgtggaaa gcgtttacca
    42121 ctgtagtagt acttccaatt tcagctacca acacatctat ttttaaagaa accttgctat
    42181 tttccattat ttattactca tttctcttct tttttcaact aagaaagtgg ctacatgtat
    42241 gcctttactg tttcttccaa atccagcatc tatgccttgt tcaacagcta tttcaggtgt
    42301 tacttgtgtt cctccacacg caatcattac tttatctctt ataccttttt ctacacaata
    42361 atcgtgtaat tttttcatgt ttttatagtg aatatcatca tgacttatta ttgtagatgc
    42421 aagtattgca tctgcattca gttcaatcgc tgcatctacc aatttttcta caggtacaga
    42481 agtccctaga tagtggcatt ctatgccata tttttcaata cctccatgtt ttatatctat
    42541 tatttctctt aaaccaactg aatgttcatc ttctccaacc gtagctgaaa ctattttcat
    42601 aggtttttct tctatatctg ctcttatttc ttcatcagtt agtaccttag gttcttctgg
    42661 tattactaaa ttgtttaaat ctatatcaaa cggaactctt cctttcactt ctattctagt
    42721 cccttcagat tcatgcataa cttctctact tatgacctca atttcttgta agttcatttt
    42781 tttgacaaat tccaaagctg caaactctgc tgttctttta tcagtaggaa gcatcattgt
    42841 tatcattata attccatctg cacaccattc catttcaggc ttaattttag ttccatttct
    42901 gtactcatat gattctttta gtctattttc tacattatca aaatcgtcaa gctcatcaat
    42961 atatattatt ttgtctggat tttcaaatgt acatccatct attaacattg atggattatc
    43021 tatagcattt tcatcatatt gagctacatt attgtatcca aagtgagcag ttacaggtgc
    43081 taaataatct tcttctcttt cataaacagt accttcacca actccacctt ttatttttct
    43141 agatattcca tcaccatttc tttctggata atttccagaa tcaacaaaaa atccttgttc
    43201 cacagattca aaatatccac caacttcaga tatttcttcc atatataata cagctctttc
    43261 tttaagttct cttgctttgt ctcttaattc accgacatct ttaagttcta tcatatccat
    43321 aagcccatcc ataccaacta aagcttgctt tgctgtatca catgcttcta tattgtatat
    43381 atgccaaggt acatttctac cttcatcagg tgttatagtt gattgtatgt cagctctagt
    43441 taatactgat gttaataagt ttaatacatg agtaactgtt gcttctctag ttgatgattc
    43501 catatattta gtattcattt gagctctcat cttataatca ctaaataact ctctaagtgc
    43561 aacagcgtaa ggtaagtcta tttttaaaca tggtgctgga ggtgctgttg gtggaactgt
    43621 tgataagcat atattacttt tatctatacc tattttttct gagaatatag agtttaaagc
    43681 gtgttgaacc ataagttcag gcattacctt ccatgcttct ctagcagtag cattagcatt
    43741 atgtgctcca tcaatttgag ctatattggc aaaagccatt attttctttg cctcacaagc
    43801 atctacaaaa gaccttacca tgtttatatt cctatacaga acattatatt gtgggtcttg
    43861 atgtgctcca ttaactcctt cctcagcaaa cattactgct acttcaggac cagcaacacc
    43921 acttacatat gaatgataat taataggtct accaacttct tcttctataa aatcaagagc
    43981 cttcctttga gctcttactt gttttcttgt tattggaact ccacctatac cttgtggggt
    44041 tccttctata agcccatcaa aatgtgattg tccagcagtt ctgataacca ttagatggtc
    44101 agctccatga tgagctgcca ttctcattct tcttatatca tcctcaaatc taccagaagc
    44161 tatttcagta gtaataactg gacttggttg agggtctata ttattgaaat acttagatga
    44221 tggtagccct acactcttct ttaaaggttt tgtacagtct ttatatttaa aaggtcccat
    44281 ttcaagattt tccaagtgtt ctctccaaac ccaacctctt cgttttggtt tatatttatc
    44341 caaatctttt aatatatagt ctatatccaa ttttttattt tcctttaaca tgattctacc
    44401 ccttctctaa ataaacatgt aacttcatcc caatattcac ccttaactaa taacaaacct
    44461 gcttctctaa ccgttatatt tttagattta gccaatttat aaactacatg ccctgctcct
    44521 ttacctataa gacctctttc cataacccct tctaaaatag gttttacttc aagagaagaa
    44581 aatcccattc tcaacaagac agacctttct attgatggtg ttgtattctt tttaccaagt
    44641 tctatcattg gttctactat ctgggtagct aaactccaaa acctttcttt taactcatca
    44701 tcactcagtt cttgaagatg ttttcttcta acttcaaaat catcctctct ttttttcatg
    44761 aacattcctc cattcgtatt gatttaatcg aattttctga atttttattt tatattaaat
    44821 taaaaaacaa taattactat aaattattta atacatcaac aacaaaatct cttgaacttt
    44881 ttacttcttc cattaaaaat tctatatctc gattacttag cttatcaata ttatcaatgt
    44941 gattattaga tatacaattt tttataaatg actttcttat tttattcata tcaatatcaa
    45001 cacatttcaa taattctggt ctttctggta atattaagtt tactcctgca atttcttctc
    45061 ttggatttcc aaattttata tctattccat tttctcttgc aaaggtaagt tgtggacata
    45121 tatgtttacc tgcacccgta tattctgttt cttgtaccac tatagcttta tcctcatcta
    45181 gctcttgagc taaagagaat gctgctgcta aagatgtatt tcctgctgga cctctttcta
    45241 taccttctag ctgtgccaat aattcagttg tataaaaaac ttctccttgt tttactaata
    45301 catatctatc catatatcta agtggtctag ctgcagacct aggtacatct gacctatcag
    45361 gccatgtaca gtatggtatc ccaaaacctg tatgtcctgt tgtaaatgat tttttattaa
    45421 attgattatc actagccatg tgtaatcctt ttaaatctac acttgctcct actactttag
    45481 tattaataga accagctttt atcaatcctc ttgcagttcc agtaagatta ccaccacctg
    45541 catttgtaca aacaactata tcagggtctt ttttatactt ttctctaaat tggatagcta
    45601 actcataacc taaagtttct acacctgcaa ttccaaatgg tgaatataat gatgcgttaa
    45661 aatagcctgt ttcttctaat aaaactaaga acgtataaaa taactctggt ccaacagtta
    45721 actgaacaac ttctgctcca tatgcctcac attttcgcgc tttttctatg atttcaggtt
    45781 gacctacacc attagaatca taacattctt gaactattat acattttaat ccctgcattg
    45841 ccgcttgtga tgctacagct gcaccatagt taccactggt agctgctatt acacctttat
    45901 atcccatttg tttggcatga tatactgcag tagctgctct tcttgcttta aaactaccag
    45961 atgcattcat tgcttcatct tttacaaata ttctagctcc tttaccttct ttagcacatt
    46021 ttctagctaa tttagtcaaa tttttaagct ctattaaagg agtatttcca acagcatact
    46081 gtgattgaat tgcttcaata tcttgtaatg tatatcctgt ttcactcatc attttctcat
    46141 aatcaaaccc tattccctct tgttcaaaag aagaataatc caatccgatt gcatttttca
    46201 ttatttcgtt atttcttccc ataacagctt gataactcat atctttcatt gatgttgaat
    46261 tattcattat atacacctcc aaataatatt tctttagctt ggattcctat ttggagtaac
    46321 tctggtatac atttaccata atcatgagta taatatggat ttattttaag taaattacct
    46381 tttacaagtc tgcctgtaat tgttttaact tctactaagt cgcctataga agcatcactt
    46441 tgtataaaac ctttaaccca catttctaag gatactttct tagtatcctc tggaacttgt
    46501 ggagctcttt cttctggagt caatactata ttgtgtataa taacccaatc attaatttta
    46561 gcatccattt tttcatcccc ttattaattg actattgcct tactaattaa tttactgtta
    46621 ttttaaatta aacattattt taccaattag ctaactgtta tcttactaat tagctagttg
    46681 ctatcttact aattagcctt tgattcttct tccaattcaa cttctatctg atttcttata
    46741 tctcccatta ttgctctagg tactggtata tccaacatag ttttaagacc tggtctagca
    46801 tttattatta atggaataga gtttactatc attgcataag tacctactcc tccaggtatt
    46861 tcaggattta tttgtaaatc aatgtttggt atacctttta ttgatacata gtctcctgtt
    46921 ttaatacctt ctgcttctgg tattatctgt tgtggatgct ccatttcaat taaaacttct
    46981 ccatttacat atccatatcc acattgtcta caaccagcaa catttccagc caatgcctct
    47041 ccgtacttag attttctatc tactgtagtc attattggct cttttgtttg ttctatctta
    47101 tctaatttcc aacctaatcc atcagctatc atatttattg attctacaaa acctacatga
    47161 cctgctatag tcttatcttc tacacctttt ataaattctt cccttgttac tccaacacct
    47221 tgttccacca taacagactt tccaaatgga gataaatcat ttactctctt agcttttata
    47281 ctagtaactt cttcacaagt acctgaaagt gccaatacta gtaaatctaa tacaaatcct
    47341 ggatttattc ctgttccaag tatacttact ccattttcct ttgcaacttc atccattttt
    47401 ttagctaaat ctgcatcatc agcttgtgga tatgccattt gttctgctgt tgatataaca
    47461 tttatttttc tatttaataa gaaatttatt ttatcaaaag cctttttagt aaatgaatca
    47521 gttgctaaca aaacaacatc tacacttttt tctctaaaaa cttcttcata atttttattg
    47581 ataataactt ctggtctttc tcctctttca attcctaaaa catcatacat acttttccct
    47641 gaagtacttc tactacaaac agatactatt tctatacctt ctttctttag aatcatatta
    47701 gccataccta ttcccatagc tccaaatccc catattccta ctcttacttt tctcatatat
    47761 acaccctcct aaaaatttat atattctcaa taattagtta aagcataaat cgtgccaact
    47821 ttaaaattaa agtaaatatt tattggaacg ttgaaactat aattatttta ttatgtattt
    47881 tcataaataa aaaataatat ttcttatgag aaataaaaaa aagagcaaaa tattttgctc
    47941 ttttataata attttgctcc tctattttgc ttttttattt tactttgatt ttgttttttt
    48001 atcttgctta tttataatat tttgatatta tattttttta ttttatactg taaattttgc
    48061 ctattcatac ctaaaattga tgcagcttta gttatattgt aagatgcctt ttctaatgat
    48121 tcatatatat acttttcttc tatctcactg ataatcttac taagtgtttt gtcctccaaa
    48181 tcgtttaaag aataattgtt actctcttta ttctctttaa gagtgtaata gtcgtttaga
    48241 ttaatcatac ttgtttctat ttgtacctca tcttccatca tactcatagt acaataaatt
    48301 atgttttcta gctctcttat gttaccttgc caatcgtaat tttttaaaat tttaatagct
    48361 tcatctgaaa ttcctaccac ttttttattt aactttttat tatactttga aataaatttt
    48421 ttgcacaata gaggtatgtc ctcttttcta tctctaagag gaggaatttt tatatgaatt
    48481 atgttaagcc tataatataa atcttttcgc attttatgct cgtttaaaat tacctttgga
    48541 ctttcatttg tcgttgcaat aattcttaca tctataggta tatccttagt tcctccaacc
    48601 cttcttacat aaccttcttg taaaaccctt aatagtttag cctgtaacat aataggcatg
    48661 gaatttatct catccaataa caatgtccct ttgttagctt gttcaaataa gcctggccta
    48721 tctattgctc ctgtaaatcc tcccttgcta gttccaaaaa gaataccttc gaatagtgat
    48781 tccggcaaag ctgcacaatt ttgtgctata aatggatagt ttctcctatt acttccatag
    48841 tgaatggact ggctaaataa ctctttaccc gttccagttt caccgtatat aaatactgaa
    48901 gcatcagact tgcaagcttt cttagattta gctattgctt ctttaatcaa attactttcc
    48961 cctaaaatat catcaaattc ataacccttt ttttgtctaa tactaacttt attgactaaa
    49021 ttttttttat aatttctatt tagtaaattt tctatcgttg tcatattctt tgatatttca
    49081 agtgccccta caaactcgcc tttaatatca attggtattg tagtattaat tgctgttatc
    49141 tcttttccat ttttatccag atatttttga atattgtctt tgataacttc ccttttttcc
    49201 aaaacttcta atagtgtact cctgtttatt ttcattttgt tgactatatc aaaaaatgaa
    49261 ttggttaaaa tatcgtgtct gttcattttt tccatttttt gtacagaatt attgtagatt
    49321 atagttttcc cgtctttatt aacaaaatgt accccttcat caatattttc caatatttca
    49381 ttcattattt taaaaaatat ctcttggttc aactatatca ccccagtacc taaatatata
    49441 aaaacccaaa aaattaataa aatgttgcta ttgtatttat cttatcatat atttatgata
    49501 ttttttatgt a
//
LOCUS       DAMJWU010000018        49357 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMJWU010000018 DAMJWU010000000
VERSION     DAMJWU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.018"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            184..1896
                     /gene="ptsP"
                     /locus_tag="PC460_001196"
     CDS             184..1896
                     /gene="ptsP"
                     /locus_tag="PC460_001196"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF2600089.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            2219..3769
                     /locus_tag="PC460_001197"
     CDS             2219..3769
                     /locus_tag="PC460_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2600090.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            3795..6497
                     /locus_tag="PC460_001198"
     CDS             3795..6497
                     /locus_tag="PC460_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2600091.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSAQQIVHKQCANDILSCIENCGLDKKYISVEVTEKVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQITKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(7003..7668)
                     /locus_tag="PC460_001199"
     CDS             complement(7003..7668)
                     /locus_tag="PC460_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600092.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            8012..9442
                     /locus_tag="PC460_001200"
     CDS             8012..9442
                     /locus_tag="PC460_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2600093.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            9810..10388
                     /locus_tag="PC460_001201"
     CDS             9810..10388
                     /locus_tag="PC460_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2600094.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            10418..10564
                     /locus_tag="PC460_001202"
     CDS             10418..10564
                     /locus_tag="PC460_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2600095.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            10869..12248
                     /gene="scfB"
                     /locus_tag="PC460_001203"
     CDS             10869..12248
                     /gene="scfB"
                     /locus_tag="PC460_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF2600096.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            12277..12774
                     /locus_tag="PC460_001204"
     CDS             12277..12774
                     /locus_tag="PC460_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF2600097.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            13072..14736
                     /locus_tag="PC460_001205"
     CDS             13072..14736
                     /locus_tag="PC460_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF2600098.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            14749..17199
                     /gene="recJ"
                     /locus_tag="PC460_001206"
     CDS             14749..17199
                     /gene="recJ"
                     /locus_tag="PC460_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2600099.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDDSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            17212..17724
                     /locus_tag="PC460_001207"
     CDS             17212..17724
                     /locus_tag="PC460_001207"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF2600100.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            17798..20005
                     /locus_tag="PC460_001208"
     CDS             17798..20005
                     /locus_tag="PC460_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF2600101.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            20030..20479
                     /locus_tag="PC460_001209"
     CDS             20030..20479
                     /locus_tag="PC460_001209"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF2600102.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            20494..21108
                     /locus_tag="PC460_001210"
     CDS             20494..21108
                     /locus_tag="PC460_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2600103.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            21199..22755
                     /locus_tag="PC460_001211"
     CDS             21199..22755
                     /locus_tag="PC460_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF2600104.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            22745..24007
                     /locus_tag="PC460_001212"
     CDS             22745..24007
                     /locus_tag="PC460_001212"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF2600105.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            24081..25868
                     /gene="aspS"
                     /locus_tag="PC460_001213"
     CDS             24081..25868
                     /gene="aspS"
                     /locus_tag="PC460_001213"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF2600106.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMLTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            26327..27559
                     /locus_tag="PC460_001214"
     CDS             26327..27559
                     /locus_tag="PC460_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2600107.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            27573..28379
                     /locus_tag="PC460_001215"
     CDS             27573..28379
                     /locus_tag="PC460_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF2600108.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(28491..29360)
                     /locus_tag="PC460_001216"
     CDS             complement(28491..29360)
                     /locus_tag="PC460_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2600109.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            29732..31159
                     /locus_tag="PC460_001217"
     CDS             29732..31159
                     /locus_tag="PC460_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF2600110.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIGSVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(31236..31505)
                     /locus_tag="PC460_001218"
     CDS             complement(31236..31505)
                     /locus_tag="PC460_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600111.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMRGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(31508..32950)
                     /gene="nhaC"
                     /locus_tag="PC460_001219"
     CDS             complement(31508..32950)
                     /gene="nhaC"
                     /locus_tag="PC460_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2600112.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(32975..34141)
                     /locus_tag="PC460_001220"
     CDS             complement(32975..34141)
                     /locus_tag="PC460_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2600113.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKYYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(34366..36486)
                     /locus_tag="PC460_001221"
     CDS             complement(34366..36486)
                     /locus_tag="PC460_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2600114.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSNNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            36719..37120
                     /locus_tag="PC460_001222"
     CDS             36719..37120
                     /locus_tag="PC460_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600115.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQIGK"
     gene            37163..37456
                     /locus_tag="PC460_001223"
     CDS             37163..37456
                     /locus_tag="PC460_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF2600116.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            37471..37911
                     /locus_tag="PC460_001224"
     CDS             37471..37911
                     /locus_tag="PC460_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF2600117.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(38003..38476)
                     /locus_tag="PC460_001225"
     CDS             complement(38003..38476)
                     /locus_tag="PC460_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2600118.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(38476..39264)
                     /locus_tag="PC460_001226"
     CDS             complement(38476..39264)
                     /locus_tag="PC460_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2600119.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            39592..40836
                     /locus_tag="PC460_001227"
     CDS             39592..40836
                     /locus_tag="PC460_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2600120.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            41159..42256
                     /locus_tag="PC460_001228"
     CDS             41159..42256
                     /locus_tag="PC460_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2600121.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            42247..42945
                     /locus_tag="PC460_001229"
     CDS             42247..42945
                     /locus_tag="PC460_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2600122.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            42948..44012
                     /locus_tag="PC460_001230"
     CDS             42948..44012
                     /locus_tag="PC460_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2600123.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITSMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            44195..44899
                     /locus_tag="PC460_001231"
     CDS             44195..44899
                     /locus_tag="PC460_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600124.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            44892..46214
                     /locus_tag="PC460_001232"
     CDS             44892..46214
                     /locus_tag="PC460_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2600125.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            46401..47774
                     /locus_tag="PC460_001233"
     CDS             46401..47774
                     /locus_tag="PC460_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600126.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEAFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(48146..49084)
                     /gene="pdaA"
                     /locus_tag="PC460_001234"
     CDS             complement(48146..49084)
                     /gene="pdaA"
                     /locus_tag="PC460_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2600127.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        19040 a         5483 c         8384 g        16450 t
ORIGIN      
        1 tataaatatt ttagaaaatg aaataagata tttttaatca taaattcaga gtaaaaatac
       61 agtaacaaat agtttataaa attttataaa aaattataag taaataaaac tataaaaatg
      121 taaattattt atataaattt ataaagttta tgtgctagat gatatttttt aggaggtaga
      181 gttatggctt acaaaggaat aggtgcttct cctggtgttg ctttagggaa agcgttagta
      241 gttgaacata gtgaattagt aattgaaaaa aaatctatag ataatgttga agctgaaatt
      301 gctaaattag aagatgcagt tgcagtttct aaagaagaat tagtaaaagt taaggaaaag
      361 gcttctgaag aattaggagc agaggaagca gaaatatttg aagcacattt attagtttta
      421 gaagacccag agttaattgg ttctgcaata gataaaatta aaactgaaag tgtaaatgca
      481 gagtatgctt taaatgaaat aaaagagatg tttgttagca tgtttgagtc tatggacaat
      541 gagtacatga aagaaagagc ggcagatata aaagatgtta caaatagaat acttagacat
      601 atattaggaa ttaaagttgt tgatttatct gctttaagcg aagaagttgt attaatagca
      661 catgatttaa ctccatcaga cacagcaact atgaataaaa aaatggtttt aggattttta
      721 actgatatag gtggaagaac ttctcatact gctatcatgt ctagaacttt agaaatagct
      781 gcaatcgttg gattaaatga tataacttca aaagttaagg atggagattt tgtggttttc
      841 aatggagaca caggtgaagt aatcgtgaat cctgatgaag agactataaa taaatatact
      901 gaattaaaag ctaagtatga agatgagaga aaagcattac agctattaaa aggaaaacca
      961 tctgtaactt tagacggaaa acatgtggaa ttagctggta atataggaac acctaacgat
     1021 atagaaggtc taataaagaa tgatgctgaa ggtgttggtc tttatagaac agaattttta
     1081 tacatggata gagattcttt cccaacagaa gaaatacaat atgaagcata caaagctgta
     1141 ttagaaggaa tggatggaaa gcctatagtt ataagaacat tggatatagg tggagataaa
     1201 gaattaagtt atttaagtat ggaacctgaa atgaatccat tcttaggtta cagagcaata
     1261 agactttgcc tagatagaaa agatatattt aagactcaat taagagcttt atatagagct
     1321 agtgttcatg gaagattaag aatcatgttc ccaatgattt catctttaga agagttgtta
     1381 caagctaaag aagttgtaaa agaagtttta gcagaacttg attctgaagg tgtggcttat
     1441 gctaaagatg tagaaattgg tatgatgata gaagttccat ctgctgctgt aatatctgat
     1501 gtattggcta aacacgtaga tttcttctct ataggaacta atgatttaat tcaatacact
     1561 tgtgcagttg atagaatgaa tcaaaagata agctatcttt acaatcaatt taatccagct
     1621 gttttaagac ttataaagac tgttatagac aatgcacata aagaaggcaa atgggcaggt
     1681 atgtgtggag aatctgctgg agaccaaaaa atgataccaa tacttttagg tatgggatta
     1741 gacgagttct ctatgtctcc aatatcaata cttcctgcta gaaagttaat aacttcagtt
     1801 aaagagtcag atatgaaaaa attagctgat gatgtactta atatgggtac tgctgaagaa
     1861 ataaaaagct atatagaaaa gacttttaat atataattaa tgttatatag ttactaaaat
     1921 tcatttttct aaataacaaa attatatttt acgaataaaa aaactatagt atatcatata
     1981 tactatagtt tttttgttat ataaattacg tatcaaaatt tgtaaaatga atagatataa
     2041 ttactaaata tgtgatagta ttggctataa taggatataa aaaataatat tgtaaaaaaa
     2101 agtttatatt tagtaatgaa aatttaacgt tttacttgaa attatgtgtt ttgtaatgta
     2161 ttattaaggc tctaatagga acattttcat caatttttct atagtggggg taggaattat
     2221 gaggtacaaa aaaagaaata tagataagtc aagtgaactt tcatttcaat ctctagcaaa
     2281 atattttata actttctata tagtaattat attaattatt ctttctatgt tttgtataaa
     2341 tacaatatgg gttatgaaaa aagataatca agatttatta tctacaacat caaatcgtat
     2401 tgaagatagg gtaggagaaa caattaagct attgaactct ttgagtaaag ataattacat
     2461 aatagataat agtgtgaatg gcatagataa aagtaaagaa ctttcaatgt atagtgatga
     2521 atttgggtat atgatgatat gttttgttga taagaatata attgtgtggg atgaagaagg
     2581 tggtaatatt agtctagctg gtcgtgatta tatgcagaaa ctttttgcaa caaaaaaata
     2641 tcaagttaca gatagttttg cagcaggggc tgatggaaaa actttaaact acactattgc
     2701 atcacctata ataaagaatg gaaatgtaga tggttgtgta tttgcagcaa tttactttga
     2761 tgagataaag gatatactca aacaaggaaa aggtcaacag caacagaatt tcatattatt
     2821 tggaagtgaa aatcaaataa tgaatttaac aagtgatgaa actgcatatg gagagaagta
     2881 tatgaagtta tcgcagaaaa caaaatatct tggaaccaat gcaaaaaaag tagaaaagga
     2941 tatgtttaat aataaatcag gtgattattg ggcttttgaa aatggaaaat tatactttat
     3001 gtcctataaa ccaattaaga acacaaattg gagtttattt tgtaaagtta atttcttaga
     3061 tatttttgca ttaaatttaa ttcctatggg agctattcta tctgttataa ctttagtatt
     3121 tattattttg atttgtatgg agaaaagctt catgaaaaaa cgaatggaag ttattgatat
     3181 gcttttaggt tctgttcaag aattagaaaa acgtatatat caaacagaaa gacctagtga
     3241 agtagacttt aaagaaatta ttcagctcac aagtaaaggt ctgactgatg gattaactgg
     3301 tgttgtaaca cgtactgtat tttttgacca aatttataat cgtgttgaaa tggaggaaaa
     3361 tgataaatta tctgtaattt catttgttga tttagatgat ttgaaatgta ttaacgactt
     3421 atatggacat gaaattgggg atatggtttt gaaaaatgtt ggatatgtac ttcgtgaata
     3481 tgagataaaa tataatggtt taattgggcg ctatggtgga gatgaatttg ttatgttgtt
     3541 atctggtttt acatcgagag aagaggtgga ggatttattg gatgagattg ctattcgctt
     3601 acatactgaa gtactaacag atgatggtat cattcaaact cattgtagtg ttggagctgt
     3661 agtatgtagt gtaaaagaca ataaagatat agataattta ttaaaacagg ctgatgaagc
     3721 tttatactat gttaaacgta atggaaaagg taattatcat ttagtttaat gtatgtggaa
     3781 ggagtttata tcatatgaaa aatgtatcta aaaaattttt tgtatgtact attattgtat
     3841 taataacatt tttatgtttt agtaaatttg ctatttatgt ttttgacaaa caagaaaaga
     3901 aagaaacaaa agtagtagaa aacatgttaa atgtctacaa gaataacatt attttaaatc
     3961 tgactgaaaa atttaattat agcaaagact tgacatcagt tttagtttca aacaattatt
     4021 ctatagataa attaaaacct attgcagatg aaattcttaa aaacaattct tgcatatata
     4081 tttcagttgt agaaaaatat aatgtagctg catttttttc taattctgtc aaaaaagata
     4141 ttatcaatac tccaatacag gaactgaatt attcttattc attagcaaat tctatcaaag
     4201 gattagtcgt agaaggtcct tctaaagacc agttttcaaa tggagatgtg tttttaatta
     4261 ctaatcctat ttataaaaat ggagagtact ggggagaaat tacagtagct ttagatgcca
     4321 aaaaagttgt agattctttt aacttaaata aattaaaaga atctggattt gagtatgaac
     4381 tttggcgtgt acacctagat aatggttcaa aatctacaat agaaatttct actaaagatt
     4441 ttaatttttc caaatcacat gaaattaagt ttaaacttcc gactgtttgg actcttagca
     4501 ttgtgtacaa aaatggatgg aataataaga atatagattt tattgtattt gttatattgt
     4561 taattatttc tatattaata ggtgtgatga catttattta cattgaaaag aagtcgatta
     4621 gtaaggaatt aaatcaacag agtcttaaaa atttagaaac tggtattttg aatagagaag
     4681 gttttttaat taagttgcaa gagcatctga atagaaaaaa taatgaaata gtgttgctgt
     4741 atatatgtat tactaattat agtaaatttg ctccagttat gagctttgaa aaaagacaag
     4801 attatcttac acatatagta aaaaatctag ataattacat aaaaagttct cattttattg
     4861 ctcgtataag tgaggaaggt tttgttctag ctttagaaga aaattgtgat aaaaatgaat
     4921 tgcttaacat tgaaaaaggg atttccttag agcttttacg aaaaattaaa gtagaaaata
     4981 aacaagtatt tttaacgatt ggttatggct catctaggtg tgttgatgat agaaaagatg
     5041 cagaatctct gttactttca gctattgaag attatgagag aagatttcca gcaatgtctc
     5101 ctattcatga ttttactaaa aagtgtgaag atatgcttga tgaaaagaaa cagattgtat
     5161 ttggtgaata tccagattta gaaataaaca agttgtctaa agtaatatac aaatattata
     5221 aaaaagtaca gcgtattatg tataaagatt ataatattga tattggaaat aggcgagggt
     5281 atatgcgtga tgtcgaagta ttaattgatt ataattctga gagaatgtta tacttatttt
     5341 taatagacat atgcaagttt ggaaaattta atgaactgtt ttctattgaa ataggagatg
     5401 ttattttaaa agaagttagt aacaaattaa aaacaatttt ttctaattat ctatatcgta
     5461 ttaatggaga tgtattttta ggcatttcaa tagataaaga atcttactta gatactttac
     5521 ataaggttaa tgaagtattt aatagttctt taagtgtaga agatgtactt tttgatgtaa
     5581 aatgtaatat tggtatttgt caatatccat atcatggtga aaatccatat gaccttctgg
     5641 aaaaggtaca aattgcattg gagtatgcaa aaaaggatgt caaaagtaat gttaaagttt
     5701 atgataagga attgacagaa gagatttacc atgaaaataa tattattaag gatttaaaaa
     5761 aaggtctgga agaacaaaca ctagaagttt ggtatcagcc aatttttaat gttagtaaaa
     5821 actgttatat aaaagctgag gcactaatta gattgagaaa taaagagggc ggttattatt
     5881 ctgcctatga agttattact tatgcagaaa agcatggact tattaaggat gtaggtctat
     5941 atgtactaaa aaaggcttgt gatgtaacta caaaaataaa agataaaaat atagactttg
     6001 aacaaatgca attaaattta tctgctcagc agattgtaca taagcagtgt gcaaatgaca
     6061 tattatcatg tattgagaat tgtggtctag ataaaaagta tatttctgtt gaagttacag
     6121 aaaaagtact tatggaaact tttgaaattg taaatgttac tcttgataaa ctaaaaaaag
     6181 aaggcattag catagcttta gatgagtttg gttctggatt tagtagtata aattatctgg
     6241 caaatttacc tattgaaaat ttaaaaatag ataaggaatt agtagaacaa attacctatt
     6301 ctaaaaaaca atttgaatta ttaaaaacta tagttcaaat tactaagatt aatggcatgg
     6361 atattgtagc agaaggtgtt gaaacaaaag aaactctgga tttagttgta acttcaggtg
     6421 tgaatttgat tcaaggtcat tatttttcag aacctttaag tgaagataaa tttattgaac
     6481 ttatgattga agaataaagg ttaataaaat aaagatttaa ggtattgaaa aataattaaa
     6541 agtatttaat aatagaatta gggaagaata agtttataag taaaaacaat tgattagtag
     6601 ttaataaaat aaatgtgata tccaaataaa tcctaaatct aatttgagtt ttaatataga
     6661 tagtctaaag tttgattatt aaagatttaa ttgtaagttg ttacagttga tgatattgat
     6721 tagagattaa agtttagagt agaaagttac cttaaattta aaaatgcaag ataacccaat
     6781 ctaaaaatta taatcactcg ataggtttaa ggatttattt gtttattata aataggaggg
     6841 tgtctcaaaa tgaacttttt agtttatgag gcactctctt ttatatagaa ttaaatatat
     6901 tttattattt caacaatgaa aaagaagctt atctctattt tgagacagcc tctttgtata
     6961 ctattattaa tttaatcaat aacataattt aaaattataa tactagttaa gatttcctgc
     7021 tttattagta aataattctt taatagcatt gtttctggaa actaaatcgt tatcagagtt
     7081 gtttgttttt ttcataatca ctgaataacg ttttttattt ataaaaaata ttaaaatctt
     7141 atcatcatca aaattgtatt ctttatatct gtcgatagac tcataaccta gatttagaat
     7201 gtttttgtct tttatactat ctacatctaa aatatatgat gggtcagcct ttagtaatat
     7261 attaattaaa catttttctt caccagcaca gaataactcc atcttacttg atatagattt
     7321 agattgctct gaagttattt ttgcaggaat aaggttgtca ttatcatctc ttagaagata
     7381 accatgtgtg tcttgttgat atactaaatt aacctttttc ttaggaacac taatcatttt
     7441 atctaaatgt acataatgat gattttcaac aaaaccttca tttaatattt caacaaagtt
     7501 attaatttta cctaggcaaa aagattctat agattcttta cagaatttat ttgtaaatgg
     7561 tattatgttt acgttttcct cgtctgtatt ccatgctata gcataatcca acgaatatac
     7621 ttcttcctct atatctgcaa aattatattg tactatgttt cctttcaaaa ttactcgacc
     7681 tccatcttat aatatattta atatacttta taaaacttat attatattta aataatatca
     7741 taaattgaat aaatatgata tagtaaataa gaaaaaattt gaaaaaaatc actataaatg
     7801 agtaataaat ataaatagtg tatactaatg ggatggaaaa tgtccactat taactttatt
     7861 accccacata tataagataa aattattaat ataaaatata atctatcgac aatgaataga
     7921 cttttaaata aacttaatat aaaattaatt aaataagtgc ttaaaaacga tttactttaa
     7981 tgatagaatc atattaaagg aggataattt tatggatata aagaataaaa ttaaatctat
     8041 atttttacca gaaaaaaatg actatttaga tgatgaagaa tttgagtatg atatagaaaa
     8101 taatgaagaa gatttaaagg aagataacaa agtacattta aatgatttaa gcagagtaaa
     8161 atatgattac agagagttaa aagacataga aaaccaaaca gaagagatgt gtctgctagc
     8221 agtaaagcag gatggtacta taattgagtt tgtaaaggac aagacttata aagtatgtat
     8281 ggaagcagta aaacagacat ataaatctct taagtatata acaaatcaaa atgaggatat
     8341 ttgtatagaa gcagtaaaac agaactacag agctttgtac tacataaata ataagacaga
     8401 aaatgtacta attgaggcta taaaaaatgc atcaacttat gatgtcatgg aagtatttaa
     8461 atttgtggaa gagcaaacag aagatgtatg tctagcattt atagaaaggg catctaaaaa
     8521 tgatgttgca gagatattaa aaggaataaa agagcaaact ccagccatat gtttagaagc
     8581 tgtaaaaaaa gatggaaaat ctttagctta tgtaaaggaa caaagtaata gtatatgttt
     8641 agaagctgta aaagaaaact acagtgcact atcttgtgta aaagagcaaa ctgaagaaat
     8701 atgtatagaa gcagtaaaac agaatgactt tgcattgtat tatgtaaatg aacagacaga
     8761 aaaaatatgt atggaagcag taaaaagaag ttatatggct ctacagtatg taaataagca
     8821 aacagaagag atttgtttgg aagcagtaag aattgatgga agagcattac agtacgtaaa
     8881 agagcaaaca gaagaaattt gcttggagtc agttagacaa aatggaaaag tactgcaata
     8941 tgtaaaaaaa cagactgaaa atatatgtat agaagcagta agaggttcat ttgaagaact
     9001 tgaaataaaa gaaatattaa gttatgtaaa gataccaaca gaaagaatat ttgtagaagc
     9061 agtaaagcaa aatggaaaaa ttcttaaata tgtagaaaat cagacagaat taatatgttt
     9121 agaggcagta agagaaaact ataatgcttt agcttatgta aaggaacaaa cagaaaagat
     9181 ttgtttagaa gcagttaatc aaagttatga agcgcttaaa tatgtgaaag aacaaacaga
     9241 agaagtttgt ctaaaagcag taaaacaaga ttatagaatg ttgaaatatg taaataatca
     9301 aacagaaaaa atatgtttag aagcagtaaa gcaaaattat agagctttag aatttgttga
     9361 taatcaaaca gaaaaagttt gtcttgaagc agtaaaacag aatagaaaag cactacagta
     9421 tgtaaaacaa aaacaaagtt aaaaggctaa aatatataga aaacatatgc aaataaataa
     9481 aatttgatat tttgtaataa ataaaatggg tatctcaaaa ggaacttttt agtttgtgag
     9541 gtactctttt tatatgaaat gaaatatatt tttagccttt taacaatggg gaaaaggttt
     9601 atttctattt tgacataggc tttttatgca tataaagctt gataaaattg ttttattata
     9661 attgttttgt tatataaata ataagaagaa aattatatag gtcttttttt gaccagtttt
     9721 acatttttaa caactagttt tgtaaagtat attgaatttt ctcaaaaaag aatgtactct
     9781 atatatgtaa aatttttttg gaggtaaaaa tgtttaaaag atatgctgaa aaaatgacat
     9841 ctgttttaat ttgtaataat atgatagata ataatgaatc taaagtctat tcttatggat
     9901 ttgaaatatt aatagctttc atagttaata taaccaccat gctgtttata ggttttttat
     9961 ttggtaaatt tacatatgta ttattttttt taatgtgtta ttgtccaata agacaatttt
    10021 caggagggta tcatgcagac aattatttta gatgcttatt aacctttatt tttataattt
    10081 tatctacaat cttaattata gaaaatataa atatagactt atttaaaaat attattatga
    10141 ttattgcaag tgttagttgg gttggaattt gtgttctgtg cccaatagaa cataggagta
    10201 accctataag tgatagggaa aaattggttt ataagaaaac tgctatattt atatctacag
    10261 tagtgttatt gataacaatt ttaagtcttt ctttgagtat atttgtagat tatttcacat
    10321 atagcgcatt tgccatgttt tggatttttg tgatgttagt attaggtaag ttaaaagcta
    10381 aagtataagt aaaatatgta tgagggagag ttaagttatg aaaaagttta ttgtaagatt
    10441 tatgaagttt gctagctcat tggcacttag cactgctatt ttgtcagcta atagtacctg
    10501 tccttggatt attcatcagc ctaaagtacc caaagaaatc agcaatctaa agaagacaaa
    10561 ttaaatacaa gcacttattt atacaaaatt atcaggaaaa aagaaattat tatattttaa
    10621 taagattaaa acaatataaa atcagataca aaatttattt tatttatttt ataggagcct
    10681 aatacctatt tggtaatata ttagtgcgcc aatttagggg gatatagtgt atttaatgaa
    10741 atgacttacg gagagtaagt catttttgta ttgtatgtat tttatgtaaa cttatgatat
    10801 aatgaagggt aaagtttaat taaaataata aaaaacttta gtatagatag taatttagga
    10861 ggtagggtat gagtatgata cataaatttt ctatgaatgg atataatatc gttttagatg
    10921 tgaatggtgg agctgttcat gtgttagatg atgttgcata cgatttgtta gatttttata
    10981 aagaaaaaag taaagaagaa atattagaga tattaaaatc aaaatatcaa gaagaaaaaa
    11041 taaatgaagc atatgaagaa atattaaatt tggaaaaaga gggtctttta tatacagaag
    11101 atacatatca atatcatcca agctttgtac atagagagcc tgtagtaaaa gctttatgtt
    11161 taaatgtagc tcatgattgt aacttaaaat gtaaatactg ttttgcagct caaggtgatt
    11221 ttggaggaga aaaagaactt atgagttttg aagttggtaa ggcagcaatt gactatttga
    11281 ttgctaattc aggaagtaga aagaacctag agatagattt ctttggtgga gagcctttga
    11341 tgaactttga ggttgtaaaa caactggttg actatggaag aagtgtagaa aaagattata
    11401 ataaaaatat aagatttaca ataactacaa atggtgtact gctaaatgat gagattatag
    11461 attatataaa tgaaaatatg cataatgtag ttttaagttt agatggaaga aaagaagtta
    11521 atgataatat gagaccaact ttaaatgata aaggaagtta tgatattaca ttaccacgat
    11581 ttaagaaact tgtggaaaaa agagcaaaag ataagtatta ttatataaga ggtactttta
    11641 caagagataa tttagatttt tcaaaagatg ttatgcattt tgcagattta ggatttaaat
    11701 taacctctgt agagccagtt gttggagatg agagtaatcc atatgcactt agagaagaag
    11761 atttacctaa gatatttgaa gagtacgaaa aatttgctgt agagtatgca gatagacaat
    11821 tacaaggaga tggatttaaa tttttccatt ttatgataga cctaaatcaa ggtccatgtg
    11881 taataaagag aataactgga tgtggagctg gaaatgagta tctttctgta actccaaatg
    11941 gagatatata tccatgtcat caatttgttg gaaatgaaga atttaaaatg gcaaatatat
    12001 ttgatgaaga aatagtactt ccagaaaatt taaaaaatat gtttagagaa gctcatgtat
    12061 atactaaaga agagtgtaaa caatgttgga ataagtttta ctgttcaggt ggatgtcatg
    12121 caaatgcgat aaactttaat aatgacatat caaaaccata tgaattagga tgtgaaatgc
    12181 aaagaaaacg tacagagtgc tctataatga tacaagctaa gttaatgtta gaaggagcaa
    12241 ccaactaaat ttatatttta aaggagttgt ttatatatga ttagagatta tttagaagat
    12301 aaacctttaa ttgacgaaag tgtatttgtg gcaaaatcag cagatgtaat tggaaatgtt
    12361 aaaataggca aagattcaag tatatggtac aatgcagtag ttaggggtga tgaaggacca
    12421 ataactattg gagaaaacac taatatacaa gattgttcta tagtgcatgg agatacagaa
    12481 actataattg gtaacaatgt aacagttggg cataggtcta tagttcatgg ttgtaaaatt
    12541 agtgataatg tgttaattgg tatgggttca ataatattgg ataatgctga aataggagaa
    12601 tatactctga taggagcagg tacactaatt acatctaata agaagtttcc accaggtgtt
    12661 ttgataatgg gttctcctgg taaggttgtt agagagctta cagaagaaga taaaaaatat
    12721 attgatgaat cttatgaatg gtatttagag gcagcacaaa atcaaaaata ttagtttaaa
    12781 gagtggttgt ttcaaaacat atttaaatct gttttgaggc aaccactaat ttattttatt
    12841 agattttgtt gatattttag ttttcaaaaa gtatttgatt aaaccagata taaaatgtca
    12901 aaaataaaaa ttaactgagt taaataattt taataaaatt ggtagtatat gtgataaaat
    12961 taaatattaa agaggatata ggatattaga atatcaattg gtgaaataat gtcatatacg
    13021 ataaattaag gtgtaagttt ttgggtatac ctatcttagg agtgaaaaga tttgagaata
    13081 agttatagaa atttaaagcg atatagagaa attggatatg ttttaataaa gtatggtttt
    13141 agttttattg tagaaagact aaatatagaa ggaattgcct ataaaatacc actatttgac
    13201 ccaccagaag aaataaaaaa tatgacaact ggtgagagaa tgaaaagagt tttagaagaa
    13261 ctaggaccta catatataaa aataggacag atattgagta caagaaaaga cttgttagac
    13321 caggatataa ttgatgaaat atcaaaactc agagatgatg tagagaaatt tgattcaaat
    13381 atagctattg atatatttaa agaagaagtg ggtttaagta tagaagaaat attcttagaa
    13441 tttaaagaag aaccaatagc agcagcttca attggtcaag tttatgaagg tgtacttaaa
    13501 acgggagaag aagtaatcgt aaaaattcaa agaccaaata tagagaaaat aataaaatct
    13561 gatttagaga ttcttagaac tattgcaaat actttaaaag atttaaaaaa ggattttaat
    13621 ttagatttag tacagatgat tgaagaattt caaactcaat taatgaggga acttgattat
    13681 acatttgaag ctataaatgc aactaaattc tctaggatat ttaaaaatag tgatgaagta
    13741 tatataccta aagtatattc agaatataat actaagaaaa ttttagtaat ggagaaagtt
    13801 aatggtacaa agttaagtga tgtagaaaaa ataaggagac taggttataa cacaaaaact
    13861 attgttgaaa taggagttag gtcatttttt acacaggtat tatctcatgg attttttcat
    13921 gcagacccac atccaggtaa tatattcgta gttgctaaaa ataagatagc atatatagat
    13981 tttggaatga ttggaattat tgataataaa actttaaacc aattaaatga aattgcatta
    14041 gcaggtgtag aaaagaatgt tgacaagata atatatctat tgatagaaat ggatgcatta
    14101 aatggagagg ctgatattaa gggacttaga caggatttac tttatctaat acattattac
    14161 tatgatattt ctatagaaaa aataaatgta acagatatat taaatgaact atttaggttt
    14221 tttagacagt ataaaatagt catgcctgca caatttgtaa ctcttgcaaa gacagttata
    14281 acattagaag gaacaagtag aactttaaac acagattttt cttttggttc tatgggaaaa
    14341 gagtttatga aacatcatta taagagtaaa tttaacccta aaaatgttgt tttaagttca
    14401 agacaaaatg tagaagaaat attactggac ataaaaacta ttccaaagca attaaaagca
    14461 attttaaaaa atatagaaag aaataatata aaaatgcaaa ttgaagatgt taaaatgacg
    14521 aggttagaaa attgtataat agagctaaca tctcaaatat ctttgagttt agtgttggca
    14581 tctattatag taggttcttc tcttataatt gcatctccaa atatagaaaa taatatatgg
    14641 ataaaattta ctgcaatagc gggtttcttt atatccttta taataggcct ttgcttagtt
    14701 ataagaagta ttaggtcaaa atacaaaaaa gattaggggg aattaaattt gaaaaaatgg
    14761 atattgaagt ataaaggtaa gatagaggaa agtgaattta gcaaaaaact taatatttcc
    14821 ccggaaatct gccaaatttt aaaaaataga gggatatgta ctgaaaaaga ttcagaaata
    14881 tttatgaatc catctttaga ttatttaaga gacccttttt taatgaaaga tatgcaaaaa
    14941 gctgttgata gaataaagtt agcagttgag aagaacgaaa aaatttggat ttacggagat
    15001 tatgatgttg atggtgtatc atctacatct atcttgtgct tatattttaa gagtataggt
    15061 tacaatgttg attactatat tcctaataga ttagaagaag ggtatggtat aaatgaagat
    15121 gctattaagc ttataagctc taggggatgt gacttgatta taagtgttga ctgtggaatt
    15181 acatcagtgt ctgaggtcaa tacagcgaat gatttgggca tagatgttat aattacagac
    15241 catcatgaat gtcaaagtga aattccatca gcctttgcag taataaatcc aaaacaagaa
    15301 gattgtaatt atccatttga ttcactatgt ggatgtggtg tagcatttaa aatgattcaa
    15361 gccttgactc caaaagaaga atttaaaact agtatgtata attatttgga aatagtaaca
    15421 ctagcaacta tttgtgatat tgttccactt atagatgaaa atagaataat tgtaaaaaat
    15481 ggtttaaaat caatgaaaga aggcaaaaat attggattaa gggagcttat aaaagtttgt
    15541 ggtgtagaaa gtgataagat aggttcttct catataggtt ttgcaatagg tcctaggata
    15601 aatgcttcag gtcgattggg atattcttac ttaggagtag agctatttac tactcaatca
    15661 caagaagagg cagtagaaat agctagtata ttggaagaaa aaaataatga aagacagatg
    15721 atagaagcta agatgtatca tgaagctgaa gaaatgctaa agtctaatag cagatacaat
    15781 gatgacaagg tgttggtttt agctaaagag ggttggcaac atggaataat aggaattgta
    15841 gcatcaaagt taactgaaaa atactacaaa ccaacaattc ttctaggtat agaaaatggg
    15901 gaggctactg gttcagcgag gtcaataaaa ggatttaata tatttgaagc tctcataaag
    15961 tgcaaagact tgatgactaa gtttggtggt catgaacaag cagcagggtt atccttggat
    16021 tcagacaatg tagaaatttt agcaaatgaa ataaataaat ttgcagatta taatttaact
    16081 gaagatgata tgatagagaa tgtaaatgtt gagtttgaat tacaagaaaa tgtaataaat
    16141 ttaaatcttg tagaagaatt acataagtta gaaccctttg gattaaataa tcctaatcct
    16201 agatttatag ttagaaatta tattttaaaa gatttgaaag ttataggtaa gaatcaacaa
    16261 catctgaaat taagcataga aaaagagaaa agctatgaat gtataggatt taatatgagt
    16321 catttaaaat caatgtataa agtaggagac aaggtagatg tactattcca attagatgaa
    16381 aataactata tgggaaatag gaaagtacaa tttttactta aagatatacg attggctcga
    16441 cctaaaagtg caagtaatga caaactttca ttgaaattaa tgtcaaaaat aataccaaaa
    16501 gatactcaaa gcctgtataa tatatctgtg tctgattttg aattatttga tggaaataca
    16561 gatataaata tatttgatta ttttgaaaaa gatacactaa taatatctaa ttctataaat
    16621 ggattttata gagcaatgtc agatatatca cttatagatt tggattttaa cataaattac
    16681 aatattattg aagatgatag caaaaatact gataaattag agcttatatt ttctcctaat
    16741 attgataaaa tagacttaaa aagatataat aatattattc tttatgatta tttatacaat
    16801 aaaggcgaat attcgtatat atatgagaat aaaagagaag aaagtgaaat tataaaatat
    16861 tataataaaa cagacctatt atatttaaag aatgtagtta gtaatatagt tccatcaaga
    16921 gatgaattta taacaattta taaacaagca ctcataaaaa aagaaattga tttggatatg
    16981 gtaaatataa gggaaacttt taatgtaatt ccattaaagt tttttacgat attaaatgta
    17041 tttagagaat taaatttact tgactttaat ttaaattatg aaaaaaattc tgtactcata
    17101 agaatattgc caaaaccaca gaaaaaatta gacttaaatg agagtttaat actcaataat
    17161 ctaaaaaatt tagaaaaaca atacaatagc agttattagg aggcgtttcc catggattta
    17221 aaaaacttta taaggaatat tgatgatttc ccaaagccag gcatagattt taaagatgtg
    17281 acaactctat ttaaagatgg agatgcattt aaatatgcag tggattctat agttgaagaa
    17341 ttgaaagata aagatgtaga tttagtaata ggaccagaag caagaggatt tttgatgggt
    17401 acacctgtag catatgcttt aggagtagga tttgttccaa ttagaaaacc tggaaagtta
    17461 ccaggtgaag ttgagagtta tgagtatgga ttagagtatg gtacagatac tttagagata
    17521 cataaggatg caattaaaaa gggtcaaaaa gtagcaatag tagatgattt attggctact
    17581 ggtggaacta tggaagccgc tgccaagctt gtagaaaaat taggtggaga agtagtttct
    17641 atgcaatttt tgattgagct aaaattctta aatgggagag aaaagttgtc taactatgat
    17701 gttaattctc ttataaaata ctagtacttt tataatagtt ggtataacac caactatttt
    17761 catatatatt aaaattttat acaaaatagg tggttatatg catgataaag aattacaaga
    17821 aataattgaa aaaataaaag tttatgcacc taatgcaaat tttgaaatgg ttgaaaaagc
    17881 ctataatctt gcaaaaaaag cgcatgaagg acaatataga aaatctgggg aaccatactt
    17941 tattcatcca ttagcagttg caaatatttt aatagagatg gaattagata ttgaaactat
    18001 aacagcaggt ttacttcatg atgttgtaga agatacagaa tatacctatg aagacataaa
    18061 aaaagaattt ggcaaagaag tagcggattt ggttgatgga gtaactaaac ttggtcaaat
    18121 aaaatatcgt tcgaaagaag aaactcaatc tgaaaattta agaaaaatgt ttttggcaat
    18181 ggcgaaggat ataagggtta tacttataaa acttgctgat agattgcaca acatgagaac
    18241 attaaaatac atgcctccag aaaaagcaaa gtataaagca aaagagacac ttgaaattta
    18301 tggtggtata gctcatagat taggaatttc caagataaag tgggaactag aagacagagc
    18361 attaagattt atggacccag aagggtatta tgatttagtt agtagagtat caatgaagag
    18421 aagtcaaaga gaggattata ttcaaggaat tgttgaactt cttaaagata agtttaaaga
    18481 tttaagtata ccatgtgatg tatatggtag accaaagcat ttttacagta tatatagaaa
    18541 gatgcaaaaa aaacataaga cttttgaaga gatttttgat ttaacagcag taaggatatt
    18601 ggttgataat gtaaaggatt gctatgcagt acttggtata gttcatacat tatggagacc
    18661 tattccagga agatttaaag attatatagc aatgccaaaa cccaatatgt atcaatcatt
    18721 acacaccact gttgtaggtc ctgatggaga acctttagag atacaaataa gaactcatga
    18781 gatgcataat atagctgaaa atggtatagc agcccattgg aaatataaag aaggtatctc
    18841 tagcaaagag tcaaaggttg aagaaaaact tcaatggttg agacagatga tggagtggga
    18901 aaaagattta aaagacccac aagagtttat ggatgcactt aaagaagatg tatttaatag
    18961 tcaagtttat gtatttacgc caaaaggaga cgtaattgaa ttaccagcag aatcaacacc
    19021 tatagatttt gcctatagag ttcatacaaa tgtaggtaat aaatgtgtag gagctaaaat
    19081 tgatggaaga atagttccta ttgattataa gcttcaaaat ggtaatattg tcgaggtact
    19141 tacatcatct aattctaatg gaccaagtag ggattggctt aatatagtga agaccccaaa
    19201 tgcaaaaagt agaattagac aatttttcaa gaaggaaaga agagaagaaa atgttgaccg
    19261 tggaaataca atattagaaa gagaatttaa gaagcatgat ataccacaaa aagaccctct
    19321 tgttgaaaaa tatatggcat tagttgctaa gaaatttaat cagccaagtg ttgaggactt
    19381 aattgccact gtaggttatg gtggtataat gtcatctcaa gtggtatcta aggtaaaaga
    19441 cctatattta aaagaggtta aaaaagctaa aaaggaacaa cagatacaag aagaagattt
    19501 aaataaacac aatataagtg aaacagaata cagtaaaaag agaaagaaaa acgcatcaca
    19561 aggaattata gtaaaagggc ttgacaatat cttagtaaga tttgctaagt gttgtaatcc
    19621 tcttccagga gatgaaattg taggatatat tactaagggt aggggagtag ccatacacag
    19681 agctgattgc ccaaatgcaa acaataatgg tgatttcttc caaaatagat tggtggaggt
    19741 ttcatggagt tcagcaggaa aaggtaagtt tgaagcggaa attcaaataa aagctattga
    19801 cagaagagga attgttaatg aaataactca tattgttgca atggataaac ttagtttaaa
    19861 cggaataaat gcaagaaaag gtaaggatag tattgtaagt ataaacttac ttgtagaggt
    19921 taatgacaca gaggaattaa aaattctaat gaaaaaatta aaagcaattc ctggtgtaga
    19981 aagtatttat agaatgacaa attagagtta aaataatagg gaggaaacta tgagagcagt
    20041 agtgcaaaga gtatcttcat ctaaagttac agtagatgaa aacactatag gtcaaataaa
    20101 taaggggtta ctagtattgt taggagtgac tcatgatgat aaatcaagtg atgtagatta
    20161 tatgattgat aaaatattga atctgagaat ttttgaagat gaaaatgaca agatgaattt
    20221 atctctaatg gatataggtg gagagctttt agtagtatct caatttactt tatatggaga
    20281 ttgcagaaaa ggaagaagac ctggatttag taatgcagct agaccagaac ttgcaaataa
    20341 cctttatgaa gaatttgtaa aaaaagcaaa agataaaggt gtgacagttg gtacaggtca
    20401 atttgcagct catatgatgg tagagttaac taatgatggt ccagtaacaa tactattaga
    20461 ctcaagtaaa tctttttaag ggggaaaact ttaatgatta taaaaaaatt tgtagatagt
    20521 tattttggag taaatactta tgtattaggt gatgaaaaga ctaaaaaatg tgcagttata
    20581 gacccaggtg gaagtttagt agagacatta agttttgtaa aagaaaatga attaaatatt
    20641 gaatatatta tccttactca tgggcatgga gaccatatag gatatgttaa ggatattaaa
    20701 gaaaaaactg gtgctaaagt agttgcacat gttgatgaaa aagaattatt aaatgacaaa
    20761 aataaaaatc taagttatac aatgagatgt ggagcgcaag agtttgatgc agatatctat
    20821 gtaaatgata aagacaagct tgaacttggt gaattaaaaa tgactttctt gcatactcca
    20881 ggacatactc aaggatgtat gtgtataaga gttgaagatg aaatgttcac aggagatact
    20941 ttgtttgctg gaagtattgg tagaactgat ttatatagtg gagactttaa ccaaatggaa
    21001 aaatcactta agaaattatg taaatatgaa gatgctgtta gagtatatcc tggacatgga
    21061 ccaaccagta ctctaggggt tgaaaagaag acaaatcctt acatgtaatt ttatattagt
    21121 atttaaaaaa gtataaaata tttaataact atattagtga gggggttctt ctactgttaa
    21181 atagaaggag ttaagttgat gttgtatgtt tttttaaaag gacatgatta taagtatgaa
    21241 gtagcagaac ttataaaatt gtttactagt gaatttaagt ttaaagactc taatgctaac
    21301 aataatatag agtcaaagca tttaataaat agattgattt atgagaatgg tgtattattc
    21361 tcaagtactg aatactatga aaatggaaac ttaaagtacg agtcaataca gaatatagaa
    21421 aatatgaatc ttgaattttt agatggattt gatagcagca aaacgattga agaatttctt
    21481 aatagcctag atagtgaaag taagaggaac tttaaaaagc tgtgtaaaga aaatatcaaa
    21541 aaaagcatgt ttttagtgct taaacaagta tttaattctt atgtaccatg gggaattttg
    21601 actggtatta gaccagtcaa aattgtccac aatttaatgg ataaaaagct agatgataat
    21661 agcataagaa ccattctaaa agataattat tttataatag atgagaaaat agatttagct
    21721 cttgaaatag ctaaaagaga gcgattattt atgtatccta tagataaaaa taaaatttct
    21781 ctttatgtaa gtattccatt ttgtccaact agatgtgtat attgttcatt tccttcaaat
    21841 tcattaaaac aatttggaca tttaaaaaga gaatatgttt ataaattgat tgaagaaatt
    21901 aaggggtttg ccaaagttat taaggatact aagaaagaag ttgaaactct atatattggt
    21961 ggtggaactc caactacact tgatgaggaa gaattggatt tgcttataaa ttcattgttc
    22021 aatgaattag atttaagtaa aattaaagaa tttactgtag aagcaggtag acctgacact
    22081 attacagaac aaaaactaag agtgttaaaa aaacacaatg ttagtagaat tagtataaat
    22141 cctcaaacta tgaatgatga taccttggta aagattggaa gagagcatag agtatctgat
    22201 ttagtagatt gctttaatat ggctcgtaga atgggatttg ataatataaa tatggatata
    22261 attttaggtc ttatagatga agacttaaat atggtaaaga acacattgga ggagataaaa
    22321 aaactttctc ctgaaagcct tacagttcat acattagcca taaagagagc atctaactta
    22381 aatataaata tggataggta taaagaacat ctaacacaat atgaagaaat ggtaaaaatg
    22441 atagatttat caatgaaata tgcaaaagat atgggattaa atccatatta tatgtacaga
    22501 caaaaacata tgttgggaaa tttagaaaat ataggttatg ctaaagaagg atatgagtgc
    22561 atttataata tacagattat ggaagaaaaa caaagtaact atgcattagg tgcaggtgct
    22621 atatcgaaat ttgtctatgt agatgaagat agaattgaaa gagtagaaaa tgtaaaaaat
    22681 gttgaacaat acattgaaag agtagatgaa atgataaaaa ggaagaaaga agaggtatat
    22741 aaaaatgttg actaaagctc ctagaggaac taaagacata actccaaaag aggcttacaa
    22801 atggcgttat gtagaaaata aatttagaga aatatgtgct ttatatggat atgaagaaat
    22861 ggtaacacct atatttgaac atacagaact tttcaagaga agtgttggag atactacaga
    22921 tatagttcaa aaagaaatgt attcttttaa agataaaggt gatagagaaa ttaccctaaa
    22981 gccagaaggt acagcaggtg tagtaagagc atttatagaa aataaattat atgcagatac
    23041 tcaaccaaca aaattatttt atgtgacacc ttgttttaga tatgaaagac cacaagctgg
    23101 tagacaaaga caatttcacc aatttggaat agaagcactt ggaagtgata ctccttcaat
    23161 ggatgctgaa ataatcgcac tagctgttca attttttaat gaagttggat taaatgattt
    23221 ggtagtgagt ataaattcag ttggctgtcc agtttgtaga aaagagtaca atgctttatt
    23281 aaaagagtat cttgactcaa aagctgatat attgtgtgat acttgcaatg agagaagaga
    23341 aaaaaatcca atgagggtta tagactgtaa aaatcctaca tgtaaagaaa atatcaaaga
    23401 tataccattt attgcagacc atttgtgtga tgattgtaaa tctcactttg ataaacttca
    23461 agaatactta aaggaaatga atataaactt tgtaattgat aaaacaatag ttagaggctt
    23521 agattactat agaaaaactg cttttgaaat tatatctaat gatataggag ctcaaagtac
    23581 tgtatgtggt ggaggaagat atgatggtct tgttgaacaa ttaggaggac caaaaggtat
    23641 aagtggaatt gggtttggac taggaattga aagactttta cttacacttg aaggtaatgg
    23701 aattgaaata gaaaaccctc aatctacgga tatatttata gtaactatag gtgaagaagc
    23761 aaatacaaga agctttaaat tgttaaaaga cttgagacaa aatcatataa gtgcagataa
    23821 agaccatata gagagaagtg ttaaagcaca atttaaatat tcagataaaa taaattcaaa
    23881 atttacaata gtaattggag atgatgagct taaaaatgac actgcgacat taaaaaatat
    23941 gaaaacatca gagcaaacta ctgtaaaatt aagtacatta gttgaagaat taaagcaaaa
    24001 gctgtaaaat aagaaagtat gatgttttgt gtattagata aatatatagc attaaaatta
    24061 aataaaatgg aggtagtatc ttggaaactt taaagggttt aaagagaact cactactgtg
    24121 gagagttgag agaaaaaaac ataaatgaag aagtcgtact tatgggatgg gtacagaaaa
    24181 aaagaaattt aggtggtctt gtatttgttg atttaagaga tacaagtgga ttatgtcaaa
    24241 tagtttttga tacagatgta gataaagaag cttttgaaaa agctgaaaaa ttaggggctg
    24301 aatttgtaat agctgtaaaa ggaaaagtat gcgaaagaca atctaaaaac cctaatatgc
    24361 taactggtga tatagaaata tttgcaactg aacttagact tttaaacaag tcagaaactc
    24421 cgcctattta tataaaagat gatgatgatg tttctgaagc actaagatta aaatatagat
    24481 acttagattt aagaaaacca tctatgcaaa gaaatttaaa attaagacat aaagtaatga
    24541 atattacaag aaattactta tcaaacaata gattttgcga aatagaaaca ccatttttaa
    24601 tagctccaac accagaagga gcaagagatt atctagtacc aagtagagtt aatccaggaa
    24661 aattttacgc attaccacaa tcaccacaat tatataaaca attattgatg gtaagtggta
    24721 tggatagata tttccaaata gttaaatgct ttagagatga agacttaaga gcagatagac
    24781 aaccagagtt tacacaaata gactgcgaaa tgtcttttgt tgagcaagaa gatgttatga
    24841 gtatgattga aggtttgctt gaagcgatat ttaaagaagt tttagatgta gaattggcac
    24901 ttccacttcc taagatgact tatgcagaag ctatgtctaa atatggttca gataaaccag
    24961 atactagatt tggatatgag ttaacagata tatctgatgt tgtttgtaac tgtggattta
    25021 aagtgtttgc tgatgctaca caaccaggaa aaagtgttag aggtataaac gtaaaaggca
    25081 aagctgacga ctttactaga aaacaaatat catcactaga agaacatgct aagacatata
    25141 gagcaaaagg tcttgcttgg atgaaagtag gacaagaagg agtaacttct ccaatagcaa
    25201 aattctttaa tgaagaagaa atgaacgcta tacttacaag aatgaatgct gaagtaggag
    25261 atttactttt atttgtagct gataaaaact caatagtatt tgatgcttta ggacaagtta
    25321 gacttgaagt agcaaatagg cttaatctat tggataaaaa tgtatataac ttactatggg
    25381 taactgagtt cccagttttt gaagaagatg aagaaacagg cacattctca gcaatgcatc
    25441 acccatttac atcacctatg gatgaagatt tagacaagtt agaagaaggt gataagtcat
    25501 ctttaagagc gaaagcttat gatattgtat tgaatggtta tgaaataggt ggaggaagtg
    25561 ttagaatatc aaattctgat gttcaatcaa gaatgtttaa agcacttggt tttacagagg
    25621 aaagagctaa tgaaaagttt ggttatttat tagaagcatt taaatatggt actcctcctc
    25681 atgctggtct agcttttggt cttgatagac ttgttatgtt acttgcagga actgataata
    25741 taagagaagt tattgcattc cctaaaaatc aaaatgctgt ttgccctatg acaaatgctc
    25801 ctacattggc ggaagatgag caattagaag aattaagtat aaaagttgat ataaaagata
    25861 atgaataata tgtagttgca agaaactaaa atgtaaaaag tctggatata aaatatattt
    25921 atattcagac tttttttatt agaattattt tatatttttt ctctatatat tttcataaat
    25981 tatacttata ttcttaacca atataattaa aatactatgt ttaaaaattt tacataaact
    26041 tgattatttt agaaactact tctgtaatga gtacacgatt ttaattaaag aatatataac
    26101 aaatttgaaa taagtctaat atatgactta atagattaaa ttaaaataat aaatttggac
    26161 tatttatcta tttataactt aaattatatc aaattgataa caaactagat attatacttt
    26221 tttgtagaat agaattacaa ttatataaag aaaaattcta ttaatagtga aaaaaaagta
    26281 gaaaaaagta gaaattattc ttttataaag aaagggaggg ataatattgc aagagagaga
    26341 agaattagtt ttaaatccag ttccaaaaga ggaaagagtt ggatggttag ctccattgtt
    26401 taatatgctt ggttctaaca tagctatatc tgaactaatg gttggaggaa ctttaatttt
    26461 aggaatgact ctttctaata tgattattac atcaattata gggaacttaa tattagttgc
    26521 aattattatg atacaaggtt atataggtta caaagaaggg ttaaatacgt atgtattagc
    26581 taagggggca tttggagaga taggaggaaa gtatcttata tctctattac ttggtataac
    26641 tagttttgga tggtttggtg tacaagcagg ggttgcagga ctatcagtac aaaagatatt
    26701 tccaagtgta aatctaacat tagttacagt tatacttgga ttatttatgg ttgtttttgc
    26761 actgtatggc tttaaagcta tggctaaatt taattactta gttataccac cacttatgat
    26821 attaatggtg tggggagttt ttaaagcttt ttctacttat ggagttgaag ctatttataa
    26881 ttatactcct caaacaacta tgagtatggt tgaaggtctt aatattgtag taggtcttgt
    26941 tatagttggt gcaattatat cacctgacca gttaagatat acgagacgag taaaagatat
    27001 ttggattatc agttttttag gtctagggat tatttcatta ttccaacaag ttgcagctgg
    27061 tgtaatgtct atgggagcac ctacttggga tataacagaa gtattggcta atttaggttt
    27121 tggatgggta gcgtttgtta ttttaatact tgcatcttgg tctacaaacg tatcaaatgc
    27181 ttattctggt ggattagcat taaaaactat ttttccaaat gtaaaaagaa atgttttgac
    27241 tttagttgca ggtctaatag gtacaataat agcagctact ggtataatat ttaaatttca
    27301 gtcattttta tctttcttgg gaatcgcagt tcctgcaata gctggaatta tgtggtgtga
    27361 gtattatttt atacaaggaa gaacttataa gcatagagaa ggaataaatt ggattgcagt
    27421 aataagctgg ttaataggtt ttgcagcgtc gtattatagt tcaaaaataa atttcttaat
    27481 acctccgata aatggaattg tagtgagtat ggttatttat tatatattaa tgaaatgttt
    27541 tggaatcaaa gataaataaa gggggcaatt acatgggtcg taaggtgaaa ataggtataa
    27601 ttcaacaaca cagtgtactt ggagatgtta aaaaaaatat agaaaaggca gtagagatga
    27661 tagatgactt aggaaaacaa ggggcagata taatttgttt accagaacta tttgctacag
    27721 gatataattt agagtcttta ggtggagtta aaacattaga attaataaga gaacataata
    27781 agtacataga ggaatcaatg tcagaagctg ctaaaagaaa taatgtatac ctgatatctc
    27841 catatggaac cttagaaaaa gggtctacac atgtttataa ttcagcagtt atatttgata
    27901 gaaaaggtaa gattatggga gaatactgta aaaatcatct gtggtcatta gaagcagtat
    27961 actttaaagg aggggaaaaa gtagaggtat atgatgctga ttttggtaga tttggtgtaa
    28021 tgatatgtta tgatgcaggt tttccagaag tatccagaga attgacatta aaaggttctg
    28081 aaataatatt tataccatct gcatggagaa tacaagatga agatatgtgg gatttaaatg
    28141 tatctcaaag agctttagag aacactgtat atacagtagg tgtaaattta gtaagtaatg
    28201 actctaattt aatactattt ggaaagagta aaatatgcaa tcctagaggt actgtaataa
    28261 ctcaattaga tacatacagg gaaaaatatg tattatcaga gattgattta gatgaaataa
    28321 aaaagtatag ggaagatatc ccctatttga aagacagaaa aagtagctat aacatataat
    28381 tctatactat aatacaagtt caatataaaa tgataaaaag aggatgtatc aatattaaga
    28441 ttaaatttat tatactttga tatatcctct ttaatattat caactttgat ttaaatattt
    28501 ttaaaatagt tgataagaaa gtttttgaac aacaacacag ctttagattc atagttttcg
    28561 ctaaaccatg aaagatttaa atatctttta aattcaatat ctttgatatg taacaactta
    28621 atctcagggt cttgagataa tccccatgat tttccacata taaatccaac tccttgaagt
    28681 gattttataa gagcataaat tgtatatggt gaatcagact caaatgctat tttgggtttg
    28741 aaattagctg attcgcacag gatatcaatt acctctctat aattctcacc cttcgttata
    28801 actataaagt tttcatctga aacttcgctt agatatactt catcctttaa tgataaagga
    28861 tggtttattg aaactcctaa tagtatttct tcgcatgtta aagttatgct attttctgag
    28921 tttagctttg taaatgaaga agatatgtac aaatcaaaat cacttttttt ataagatgat
    28981 ggcaaagtat gtgacacatt aaaagtaata ttgggatata gttttctgaa attactcaaa
    29041 agtgatggta acacattagc cgctgcagga gataatatct ttacttctcc tgaattttct
    29101 aaagaagtgt cttgtaactc cttttttgaa ctttctacta aatctaaaat gctatctact
    29161 ctttttagaa acaatcttcc atactcattt aaaacaatat atcttccttt tctgtcaaat
    29221 aactgaactc caagttcttt ttctaaggaa gatatagttt tgcttaaagc tggctgtgct
    29281 atatttaact tgtgtgatgc ttttgttatg tgctccattc tagcaacagt ttgaaaatac
    29341 ttaagatgca ataaatccat agtataaaat acacctcttt taatatgact tgagttatat
    29401 caattttata atcaactcca cattatacat tataatataa agtttatata ataaactaca
    29461 atatattaaa aagttattta aattgttatt tatattttgt acataatcta attaaaaaaa
    29521 gtaactttaa atgattaact aaggaaatgt tgttacaaac tttagttata taaaaaattc
    29581 atttgatgag caaagtacta atatcaagta agaataaaag cttattagat aagaaaaata
    29641 atattttata ataatgatgt tacttaaatt attatgttat actattttaa ataggaaaaa
    29701 tttagatact ataattttag ggggagtcaa gatgaaatta actaaatcaa tatcatcaat
    29761 gctgtgctta tctctagttt taagcttatc accagtagca tatgcaaatg aaataagtag
    29821 aacaacagtt gagaatactt tagtaggtca ggatagatat caaactgctg ttgaagtaag
    29881 taaaaaagga tggagtagtg caaatgaggc tgtagttgta aatagtgaat ctattgtaga
    29941 tgcattagct gtaacaccat ttgctaaatt aaaaaatgct cctatattat tgacagaaaa
    30001 aaataatcta aattcacaaa ctgagaaaga attaaaaaga ttaggagtta aaaaagttta
    30061 tataattggt ctatctagtt ctgtatcaaa tgatgtgcaa aaacagttag aatctaataa
    30121 tttaactact gatagagttg gtggtagtga tagataccaa acagctttga gtatagctaa
    30181 aaaaatagaa ggattaaaag atatatcaga aatagctgta gtaaatgggt acacaggtct
    30241 tgcagatgca gttagtatag gttctgtagc tgctactaat ggaatggtta tacttccagt
    30301 atctgatgca tctggtatat ctagctttaa agattttata gaatctaaga atataaataa
    30361 atcatacata ataggctcta ctaatgctgt atcagataaa ataaaacaaa gtttacctaa
    30421 ttcagaaaga ataggtggag cagatagaaa tgaaactaat ggtaaagtaa tagaaaagtt
    30481 ttatacatct aataaattaa acaatgtatt tgtggctaag gatggaatga aaaatcaaaa
    30541 tcaacttata gacgctctag cagtaggagt actagcatca aaagaaaatt ctcctgtact
    30601 aatcgtagga gaacaattaa gtgcaacaca aaagagtgta ttttcgaata aaaatgctac
    30661 ctctataact caagttggta gtggaggaaa tgaaaatgca tttaaagaat taaaagattt
    30721 acaggcaaat agtaacaata agagagtaat gtatgtaact aatacagata aggtaaatat
    30781 aagaaatgat gctacaatag aagcttctgt tataggatat ctaaataatg gtgatgaagt
    30841 agaagtttta gatgtgttaa aaactggatg ggtaaaaata aaatacaatg aaggtattgg
    30901 atatgttagt gggtcttatt tgacaaataa taagcctgat aatagtaatg aaaatataaa
    30961 aataaagtat gtaaaagaaa aagatggtct aaatgttagg aaaggaccaa gtacagaaga
    31021 tgaaaaaata ggtcaccttt catatggaag caaagtagaa acaatagaaa tgtttgctac
    31081 tggatgggta aaaataaagt acaatggtgg atatggttat gtaagtaatg attacttatc
    31141 tgatacacca gttatttaat tataagtaac aataaaaaaa ctgctttaat ttgtaaagca
    31201 gtttttttat aaaaatttaa gtctaataac tatttctatt ttgatacctt aatcctggga
    31261 tttattatta tggatattat caacatacaa acactccaaa tccagtgtct aattccaaac
    31321 ttagatatta ataaattcag tcctgctatg gtaaataagc agtatttaat tacaattaaa
    31381 agtgtattta tagaaagccc cctcatagaa agtatcctaa atccaataat agacattcta
    31441 agtcctataa atgccaataa tatacctgca atatcctgaa ttaagtaata gtattcaaat
    31501 ttcatattca atcaactctt ctctgagcag tatcaaatat ttctaactct tcaatttcat
    31561 catctgtgta tgtaagcata gtaaaaccag ttacaccata aataaatgaa attataggtg
    31621 taatccaact taagaaacaa tatggaataa attctagtgg acttacacca agagtctgag
    31681 cacagaaaac agcatttgaa ttccatggta ttaatggacc accaagtgta gctgtatctt
    31741 ctataattct agatagattt tgtggtttta atttaaattc tttgtatatt ggagacatta
    31801 gagttccgac aaatacagaa gtgaacatca ttgatgaact aaatgcattt gcaaagtagc
    31861 ttactataaa agtagaccca accaatttag ttcttgattt tcctatttta gttataagag
    31921 gttgaagcag tacttctaag aatccagttt tttggagaat accagcaata acgaaaacta
    31981 caaatactag taaaacggtt tcagccatac tcattattcc accacgattt aatagtttgt
    32041 cggcatagac aaatccagta tctatagtaa atcctgatag catgtaattt aaaacatcac
    32101 ctattttttc tccttcttga aatactgcta taataagtcc catgatagct gaacttagta
    32161 tagatataat gggtggcttt tgtagcaata aaagtaataa taaaaatatt attggaatca
    32221 ttgctactaa accaatatga aagtttgaat ttaatacatc cttgatttga tttatttgaa
    32281 catagtctat agtattattt gaatacttaa atccaattac tgtatacaga actatacata
    32341 ttacgtatgc aggtcctgtg gtataaaaca tatgcttcat atgtgtaatt acatctgtct
    32401 tacaaactgc tgcagctaag tttgtagaat ctgatagagg tgacatctta tctccaaaga
    32461 aagcaccaga aatgactgca ccagctgtta aaccaagagg aatacccata ctagtaccaa
    32521 tactcatcat tgctatacca gaagttccaa gagtccccca agatgtacca gtagcaacag
    32581 aagttaatga acatacgatt aatgttgtaa gcagaaaata tttaggggtt attattgtta
    32641 gtccactata tactactgtt ggtattgtac cagcagctat ccaagctgct attagtacac
    32701 caacagaaag tataattaag tttgattgaa aggcatcttt gccaacttca aatccaaaag
    32761 cttctatttc atcattagta taaccaagtt tcatggctgc aggaactact ataagccatg
    32821 aaagtaaaaa cattgtagtt atagaagcat taaatacaac tataccaact gatgttagag
    32881 ctattattgc aagcattact aacaaggaat atctaaatga aggtattttt ctttctgtat
    32941 tcattttcat agtgcaaatt ctccttaaat atttttagtt aattgcattt tttattctta
    33001 taagagcttc ttcaagcata cttcttggac aagctaagtt tattctttga tacccacttc
    33061 ctccaatgcc aaagctgttt ccttgattta gagcaacttt acctttttgt acaagtatac
    33121 tttctagttc ctcatcactt aatccaagtg cactaaagtc tacccataat aaataagtac
    33181 cttctggttt tctaacttta agttttggca tattttcatt tatatatttt atagcaaaat
    33241 ctatattaga ttctaaatat tctaaaaatg attctagcca agattcacca ttattatatg
    33301 aagcttcagt tgcaactaag ctaaagcaat tatttctctt tatatcaatt ctggtaaagg
    33361 catcatctaa caatttatag tatttttcat caggtagtat tacatatgat gattgaagac
    33421 cagctatatt aaaagtttta gttggagcca tacaagtaat tgtatttttt tcaaattctt
    33481 tagaaataga tgccatagga atatgcttat gtttttttaa tataatgtca ctatgtattt
    33541 catcagatat gatttttaca ttatgcttaa gacaaatatc tcctagtttt tttaattcat
    33601 cttttgtcca tactctacca actggattat gaggattaca aagtatgaat aattttacat
    33661 ctttaatctt gttttcaatg tcttcataat ccattatgta gtttccattt tctaattttt
    33721 gtaatgggct tataattagt tctctattgt tattttttac aacactatta aaagggctgt
    33781 aaactggttc ttgtatcatt attttatcat ttgcttttgt gagttcattt attaaaagac
    33841 ttatagctgg gataacacct ggactataaa ttaaccactc actttttatt ttccagttat
    33901 gtctcctatc tagccagttt actatagatt cattgtaaga atctggtctt gttgtgtagc
    33961 cataaatctc ttgttctagt ctatttttta aagaatcaat tatacaaggt gcagccttaa
    34021 aatccatgtc agccacccac ataggaagta aatcattagt tccatacttt ttttccattt
    34081 ctgaccattt agatgaaaaa ttattgcttc tatcaacaat ctcattaaaa ttataattca
    34141 ttgtaaatac ctccagtaat tttaatttta tgttaataat attaagcaaa caacgtgcca
    34201 atattgataa taaaaaatat ttattcacaa gttaatattt aatattaagt aatttcagag
    34261 catagataag ataaaaatat taattatact taaaactttg tggaaaaggt tatttctaat
    34321 aaatttttaa atatatgaaa atgtaattaa ataggttaaa taggtttaac tgtttaactt
    34381 gttagaccta tttatatttt ttagaaactc tactcccttt gatgttataa ttgaacctcc
    34441 acgaccaaca agtatgtcta caagcccaaa gtcttgtagt tctaaaagca tatttctaat
    34501 ctgttgttct gtcaaaaatc tattttcctc taaagctatt ttacataagc tttttctacc
    34561 tattctttct tttaaatcat aagccttttt taaattatcc aatataaata tgtattcatc
    34621 tagtgacctt ttaaaattat agtcgtattt catgatgttt ttgtctttgc caatgttgaa
    34681 ttgatacttt tttatattat tacttacttt attacaaacg gttctactat aatttgaatc
    34741 tatagtatct aatatatatt caggtaaatc acatatttct ataatatctt tttccaagta
    34801 acagaaatat tctcctaagt ttcttaattc tcttacattt ccttcccatc tatacatttt
    34861 aaagatttct tttacttcct cacttagaat aaaattacat ggaatatcat attttaatga
    34921 accaaaaatt tcaaatatat cagcaggtct ttccctaagt ggatgtattt taaggggaag
    34981 aacattcaac ctaaaatata aatctcttct aaacatattt ttagatacaa gttcttttaa
    35041 atttctatta gtagctgcaa ttattcttac atcaatgtca atgacattat tagaaccaat
    35101 acgtcgtact tgtttttctt gtatgactct aagtaattta gattgagaat ttaaatccat
    35161 ttctccaatt tcgtctaaaa atatagttcc attgttggct aattcaaata aaccaatttt
    35221 cccacctttt ttagcaccag taaaagcccc ttcatcatag ccaaataatt cactttgcag
    35281 tagattttct tgaaaagtac tacaatttac tgcgacaaaa ggaccatctt ttctcctaga
    35341 agcactatga atggattgag caaataattc tttaccagta ccactttcac caattataag
    35401 gatagatgaa tttgattgag ccattttatt agcaattttt tttgtattaa tagtttgtat
    35461 actagaacct aatatatcat caaatgtgta tttactgata ttaccagagt ttactagttg
    35521 tgctcttaat ttagcttgct ttttttctaa ttgagaaaac tttgtcatct ttagtataaa
    35581 tccatcaaat acatttaatt gtatatgttt aatctctaaa tttatatcaa tatgatttat
    35641 tttaatcaat ttctcaaaaa atggagtttt attggtcaat atgctttgaa aattgatgtc
    35701 ttttatgcaa tcaccaacaa atgagtcaat catttcattt gcatttatag acaaaatttt
    35761 tctagccata tgattataga attgaattat tccatcgtta tttgtgcata taattccatc
    35821 atcaattata gatagtaaca aatcaaattg tctttcaaac ttatttgcat ctaacaataa
    35881 ctcacctgta ctataacttg taggaattat tttttctacg tattttttta ttaggtcatc
    35941 ttttattaga tgttttaatt tggtttttgt agcaatttca acaatacaac ttaaatctat
    36001 tattctgtat tttaaatcta taacttcaca atttttaaac ttaggcaaga ttttttcgcc
    36061 aggagttagg attacagcat ctgtgggagt aaactcaatc tctggatagc agggcaggat
    36121 atttatattg tctattccta atctatgtat taaagaaatt gtttctattg ccatatcttt
    36181 acttaagtta tatacataaa taattttatc tttttctaat ttggatatca tatcaatact
    36241 gttcttttca aatgtcaact taggaacaat gatttgagca tcatagtgag ataattttac
    36301 tatttcatca tattttaaat aagcagatag gagtataagt ttttctttaa aaaactttaa
    36361 atttccttct tcaaatgagt ataaatttat ctttagatta tcacctaaga attgtcttat
    36421 ttgattttca taaatttctc cagcgtcttt ttttaaactt acaatagcta attccatttg
    36481 tttcatttta acctctcata caaatttgac ttattttaga ttacataata attaaattag
    36541 attaaataat attatttaat tattattata aatttattat agactacaca ttgaagtaaa
    36601 agaagtagct aaaattattc cttttatttt tgttttttgt atctatttgg tataatagta
    36661 ttagagcgta taataattta ggcaaaattt tttaaggaag actagaggag aatataaaat
    36721 gaaaaaatgt ctttactgta atagggatat aaagtcacca aataataaat tagctataaa
    36781 atatgatgac aatatgaata tatatccatg tcgtgcagaa tgtaaggaaa aaattgaaga
    36841 atatatagca aaaaaaccta catataagtt tataaatata ttagaggttc ttttaggtgt
    36901 gggcggaata ttctgctttt tgagtaaatg gactattgca gcccaagttg tacttggaat
    36961 tgatgcactg gttatatttt tattcccatt ggcaggattt aagatgaatg gaaagttaag
    37021 tatggaacaa actaagaatc aaagtaggtc tattgcaata tcaattttgg catttatagt
    37081 aataataact gtattatatt ttaaacaaat tggtaagtag agcataattg tataagacat
    37141 cattagagta tgaggtgagt ttatggaaga aagaaaatta gcaccaaaca atgagaggga
    37201 agctctcata aaaagattaa aaagaataga aggtcaagtt aagggtatac aaaagatggt
    37261 ggaacaagaa cgttattgtg ttgatatatt ggttcaaata tctgctataa ggtctgctat
    37321 aaatagagta ggcacaatta tcttagagaa tcatataaaa ggatgtgtag cagaaagcat
    37381 taaacatgat gatgctgata aaactgaaga gatgataaaa gagcttatgg atactataaa
    37441 taaatttaca aaataaggag gcggttagtt atgaatcaac aaggatttat tatggaaatt
    37501 gttgatgata gaactgcaaa gttaaaaatg caaagacatt cagcttgtgc tgcttgtgga
    37561 aaatgtgcta ctacaaattc agcagagtca aaagaaatat tagtagaagt agacaacaca
    37621 attggcgcaa aagttggtga ccatgtagaa gtgagtatgg ataatatgaa tgtattaaaa
    37681 gcagctgtaa tggcttatat aatacctctt atagcacttc ttgtaggaac aatagggact
    37741 tattatatat tgggggcaat tggaatgaca actggagttg aagccataag tggattagtt
    37801 ggaatttgtc ttacaataat ctgttattta tacttaaaga agaatgacaa aaaatttaga
    37861 gacagtagag aatttatacc agtaataaca agaatattaa ttgaccttta agataattat
    37921 aattaaaaaa tagcaatgtg cttcttaaat aaatactcaa tacgtatttg tagagtttaa
    37981 gtacattgct atttttatat ttttattttg attttctgta aggaataaag ttttttctga
    38041 ttttcaaaag attcaatgtt cttacaaaag aagttacaac taatacacca agtgctattg
    38101 cagcacttag aaatagcgca tctgtacctt ttgacatagc caaagaataa tttttctgaa
    38161 tcaaagacaa cattgtatta tatagcccaa ttcctggtat tagaggaaga atacctggaa
    38221 ttacgaacac aatagctggt tgctttagtt ttcttgctaa agtctcactg cacgctgaga
    38281 ccaaagcagc agcgataaaa cctgcaaaaa ctgcatttgt agtaaaatta aataaataaa
    38341 catatacaat ccaaccaatt ccgccagtta ctccagctgg aagaagtaga tatagtggag
    38401 cattaaagaa aacagcaaat ccagcagttg caacacctga aaataaaaaa tgcaaaatca
    38461 tagacatatc agtcattaga aaggacctcc tattctatac catgtatcaa ggacaaaacc
    38521 aactccagaa gcaattgcaa taataatcat agcagcatca aaagcacgag aaattcctga
    38581 tattaaattc cctgatatta aatctctaat agcttttata aaagcaactc ctgggagtag
    38641 tggcattata gcgccgacta taagcatttt agggtcatct aaaatgccta tttcaactag
    38701 tagaactcct gaaagtgcaa ttaaaaaaga agatactaaa caggaaaatg cagatattgc
    38761 acttatcttc atcgttttat cataagtaat tgcagcaaag attgctatta taaacgtaag
    38821 tataaaattt aatacataat tccctccaag caaacaagca aaacaagcag atgcaattcc
    38881 tgtacaagag taaaataacc ataaaggata aggttttaca tcttggattt cataaagacg
    38941 tgcaatggca ttatcaatat catactcttt gtcactaaca aattctcgag aaaaactgtt
    39001 taataaagaa atcttattta agtttatacc tcttgattga atcgttttca taaacgaaaa
    39061 gccatcaaat ttctcatctg aaataattat tactgtagga gttgtaaaaa tatttacatg
    39121 ataaaaccct cttgacctgc atattctaag gacactgtct tcaaccctag atgtttcagc
    39181 tccatttttt atcatcagtt cccctacaaa tagagctagt tttagaatat ttttcttata
    39241 ttcaagttcc tcattttgat tcataaatac acctccaaat taaaaaacgt aatattgaaa
    39301 ttatatcaaa tttttaagta atattcctta atatctatac aaatatatta tctttttttt
    39361 gtttcaaaat agtcacattt aaattatatc caattaaaag aattagacaa tattatttat
    39421 tatattttaa cttaaaaata aagaaagtaa ctatgttaaa tatggaaaaa tggcaaaaca
    39481 cttgactatt aattggtaaa ggactataat aataaattat catattgata aaaacgaaaa
    39541 ttatgaaatg tctgtaaaaa taaaatttaa ataataacta ggaggagtaa tatgtttgag
    39601 aacttgaaaa aagcggaccc tgagatttat gaaagtatga aaagagagtt aaaaagacaa
    39661 caaagaaata ttgaattaat agcatctgaa aacatagtat cagttccagt aatggaaact
    39721 atgggaagtc atcttacaaa taagtatgca gaaggttact caggcaaaag atattatggt
    39781 ggatgtgaat acatagatga agttgaaact cttgcaatag atagaatcaa aaaaatattt
    39841 ggagcagaac atgctaatgt tcaacctcat tctggagcaa atgcaaatat tggtgtatat
    39901 tttgctatgt taaaaccagg tgatgttgtt atgggaatga atttatctca aggtggacat
    39961 ttaactcatg gtgctccagt taatatttct ggtcaatatt ataaatttta tgaatatggt
    40021 atagataaag acagtggact tatagatttt gatgaagtta gaaagatagc acatgaagta
    40081 aaacctaaaa tgatagttgc aggagcaagt gcatatccta gagaaataga ttttaaaaag
    40141 tttagagaga tagctgatga ggttggagca ttgcttatgg tagatatggc tcatatagca
    40201 ggtcttgtgg ctgctggtct tcaccaaaat ccatgtgaag tggctgattt tgttacaaca
    40261 acaactcaca aaactttaag aggacctcgt gggggtgtta ttctttgtaa agaaaaatat
    40321 gctaaagaca tagacaaagc aatattccct ggtattcaag gtggacctct tgaacatata
    40381 attgcttcta aggcagtttg ctttaaagaa gctttaagtg atgagtttaa agaatatcag
    40441 gtacaagttg ctaaaaatgc aaaagcctta gctgaagaac ttattaaaag agattttaaa
    40501 ttgatttctg gaggtacaga taatcattta atactattag atttgactaa taaaaatata
    40561 actggtaaag cagcagaaaa gagattggat gatgcttata taacagctaa taaaaataca
    40621 attccatttg acccaaatgg tgctcttgtt acaagtggaa taagacttgg tactccagct
    40681 gtaactacta gaggtatgaa agaagaagat atggcaataa tcgctgaggc tatagaccta
    40741 tgtttaactt atgatgaaga atctaaggct agaacattgg ttgttggttt aactgaaaaa
    40801 tatcctttat atgaagaata taatattatg gattaattat aaagaacaac taaataaaat
    40861 acatttaaat aattacaatg attgtgtatg tatttgaata tatttagata taaaaaaggt
    40921 gtctcaaaat gaacttttta gtttatgagg cacttttttg tatggaatcg aacatatttg
    40981 tattatttaa ataataaaaa atatggttat ctctattttt agatggtctc tttgtatttt
    41041 ttaattaaaa ctttaagatt tcttaagaat tatactaggt ttttctaaag atttaaagtc
    41101 tatacttata agtatagtaa tcatatgaaa agaaaagctt taacgaggag ggaatcttat
    41161 gaaggtatta attcttacag gaaaatttgg tatgggtcat tactcagcat caaattcttt
    41221 aagtgaagat ataaaagcaa aatttgataa ttcagaaata ataattaaag atatatttga
    41281 gtacattatg cctaactatt cagataaaat gtacaagact ttttctattc ttgtaaatcg
    41341 gggaagtagt ctttataatt tgttttataa atgtgctgaa aatggaaaga aagatataaa
    41401 atttactttt tcagattatt ttttaaataa attagataca ttacttcatg aagtacaacc
    41461 aacagtagtt atttcaactt ttccattttg ttctcaatta gtatcaagat ataaggaaaa
    41521 atataactca aatcttcctt taataacatg tataactgat ataagtagtc actcagaatg
    41581 gattagtaaa aatacagact gttatttagt agctagtaaa tctactaagg aagaacttgt
    41641 ttttaaagga atagatgaat ctaagattaa agttaatggt attccagtaa aaaaagaatt
    41701 taaaagaata gaacatgtaa atcattcaac taaaaaaaat attcttataa tgggtggagg
    41761 attaggatta ctgcctaaat cagaacaatt ttacaaagaa ttaaatagtt tagaaggagt
    41821 aaaaactact gtaataactg gtaataataa aaaaatgtac tataaactat atggtagata
    41881 tgaaaatata gaggtagtag gttacaccaa tgaagtatat aaatatatga aagattctga
    41941 ccttattatt tctaagccag gtggaataac actatttgaa acaatttatt cagaacttcc
    42001 aatattggca tttaatccat ttttacaaca agagatagat aatgctagtt ttatattaaa
    42061 taatgaaatt gggagaatac ttggtaaaaa taaaaaatac tatgtagatg agataaaaga
    42121 tttaatttat gatgatgcta ctcttaaaga aatgagcagt aacatgaaag aattaaaaaa
    42181 acaatttgat aataatacct tagagaatat tttattttca cttgatgaac aaggagcgtg
    42241 tagagaatgt atgtagtagg tttaattatc gttgttgcat tgattttttt agtccattca
    42301 attataccta catactataa taaactatta aataaagagg tattaaaaaa tatggctgga
    42361 gaaaatgaaa ttgcacttac atttgatgat ggtccagata agagatatac agaaaaatta
    42421 ctagatgtgt taaaagaaaa tgatatacaa gctatgtttt ttgtagtagc aaagaatgct
    42481 gaaaaagagc ctgaaattat aaaaagaatg ttgagagaaa atcatatagt tggtttacat
    42541 tcattggaac acagaaatgc atggctttat agttatagtt atgttaaaaa agattttatt
    42601 gaaagtacaa atataatgaa aaatttaggt gtagatgtaa actactatcg tccaccatgg
    42661 ggtcatacta atatattttc aaattcattt gttaaaaaat ataatctaaa aatgactttg
    42721 tgggatgtaa tggcagaaga ttgggagaaa gatagtacag tagacattat tataaacaag
    42781 ttaatgagta gaacaaaaga aaattctata atttgtttac atgatgcagg tgaaaattca
    42841 ggtggtgcag taggtgcacc tgagcgtact attgaagctt taaagatagc tattccaaaa
    42901 cttaaagcca gtggtttaaa gttcgtaaca ccagaaagga tgtagttatg ataagtaaaa
    42961 ctgaaaagaa aaataaatgc ctaggtagtg tagctttttt agttttacta atgggcataa
    43021 caggatattt tgtttttaga gggcagtcag ttgaatcatt gataaaatca ttaaaaggtg
    43081 caagccctat gtttatacta attggatttg caatgatgtt tatatatgtt gcctgtgaag
    43141 gtataaatat atacttaggt atgaaagctt taaaccaaaa gactactctt ttaaaatgta
    43201 tgggatatgc ttttattgga ttttatttta gttctattac gccttcagca tcaggtggtc
    43261 aaccagcaca agtttattat atgaaaaaag acgatataaa tatatcatat tcttcactta
    43321 tactattagt cattgttgtt attcaccaag tagttatact ggcatatagt ggaataatgt
    43381 tcattatgga acgtgagttt atattaaata atgtaagtgg tatgaacatt ttactaatat
    43441 atggtgtaat aacaaatgtt gctcttgtca ttggagtaat tgcaattata ttttcaaaaa
    43501 aacttgtaaa caattttata atatcaataa caaatttatt aggtaagtta agaataataa
    43561 aagatgttga aagttcaaga aaagttataa tatctcaaat tgaagagtat gttaaaggtg
    43621 ctcaatatat aaagcagaat ccaaaacttg tagtccaaat tttagtcata actattgtac
    43681 aaataacatc tatgttttta gttcctttct ttgtatacaa agcttttcat ctatcaacat
    43741 atactgtatt tgagatatta gcgatacaat ccttgcttaa tattgctgtt tcttcattac
    43801 cattgccagg ggctgttgga gcttctgaaa atagttttat gacattattt aagatatttt
    43861 tccctggtca cttattagtt ccggcaatgt tacttagtag gggaataagt ttttatgcat
    43921 ttgtagctat aagtggattg atatgtatag tagtacatgt aaaatcttct gaaaaagtaa
    43981 aagcagtgaa aaaagttgct tatgtaagat aatattatat agattatttt tattaatatg
    44041 tcaatttgtg tgtatatttt aattaagctt taaacatgtt tacaggagta gaaactatta
    44101 aaacgtgtga cattgctaag cttttgctaa taaagtataa tattttatat agtttgaaat
    44161 atttaagaaa tatagcaaaa aaaggagctt ggtgatgaat aaagagattc caaaaaaaat
    44221 tttagtagct gatgataatc ctgagattag ggaaatcgta gaaatacttt taactggtga
    44281 aggttatgaa gttattatgg caacgaatgg agaagaagct gtaaacttag tagatagtac
    44341 gattgatttg attattttgg atgttgtaat gcctgtaaaa acaggatttg ttgcatgtaa
    44401 tgaaattaga gaaaaaacga cagcaccaat attattttta actgcaaaaa ctcaagactc
    44461 tgataaagtt attggatttt cagcaggagg agatgactat ttatcaaagc ctttttcata
    44521 ttctgaacta atttcaaggg ttaagtcgct acttagacgt tattatatat accagggaaa
    44581 ggaaaaggaa agagagccaa atatattaaa aacaaaagag ttatgtgtca atttagatac
    44641 tcaagatgtt acactaaatg gagaacctgt gtttttgaca actatagaat atagtatatt
    44701 aattcttatg ttgaaaaata gaaaaaaagt tttttcgtct gaaaatttat atgaaactat
    44761 ttgggatgag cagtattttt atacagcaaa taatacaata atggttcata ttagaaattt
    44821 aagaaaaaag ttagaaaaag accaaaaaaa tccacaatat ataaaaaccg catggggaaa
    44881 ggggtattat attgattaac aaaattaagc catctaagtt gggagttata ctattgatat
    44941 atgtagtaat ctcagctgca gtggctttca ttttaaatat accattttac tctgctgttg
    45001 aatctatagt aaaagatgta tcagaaggga aagtatattt taaaagagaa gtaaatcaac
    45061 ttatggataa ttttcaaact tatgtgaata acaatggtat aagagcagat gatggagatg
    45121 atatagcaaa atggaatagt gataattggt ttgtttctat gaagatatat aaaggaaata
    45181 aggtttttta tgatacatta tactatccta atgaagtacc tgataagagt gaggaaaatg
    45241 agttttatag cagtcccatg gctagaaact atgagataca attttctgat attaaagcta
    45301 atatagttgt agtagcttta tttagattta gatttcaaaa ttttatagaa gcaatttcta
    45361 ttacatttat ggcacttaca tttattataa catttttact gttgtttagt agaaagataa
    45421 agtacctaag gaaaatagaa atgggtataa agatagtaga gagtggcagt ttaaagtata
    45481 gaatcccagt aaaaggtaat gatgaattat catcacttgc atctagtatt aatgagatga
    45541 gtcatacatt ggagaatcaa cttataaaag aaagggagat gaagaataaa aataaagata
    45601 tagtaacttc gatatcacat gatgttagaa ctccattaac gtcagtaatt tgttatttag
    45661 atttgattaa agaccataaa tacaatagtc ctgagcaatt ggaacaatac ataaaaaatg
    45721 taaggtctaa agcatatcaa ataaaagatt taacagatga cctttttacg cattttgtta
    45781 attctggaga ggaagtttca aaagggtatg aaaaaataaa tgctaatgaa tttatttttc
    45841 aattactttt agatagttca tatacattgg aggagaaagg atttcatata acaatagaaa
    45901 atgaacttta taaccaatat catatttatg tagatgttac acaatttaga aggatatttg
    45961 ataatttatg ttctaatata ataaaatatg ctaggaaaga taagcctata gtatttttaa
    46021 ttaaagtaga ggattctaaa ttactaatta tacaaaaaaa tagagtaagt aatttttcta
    46081 agaatgtaga aagttttgga attggtataa agaattgtga gaaaatagtt gcatatcata
    46141 atggaagcat aaatgtaaat tcagatgata aagaatttgc tattgaaata tctattccag
    46201 caaaagaatc ataaaaattt aaattatata ttgtgtaatt gctattatat attgtgtaat
    46261 tgcttatgta aaattctatg aagcaggaag catgtgactt tagttatggg atgttcaata
    46321 ttgtgtacct ctaggcaagt aacaagttta cttacaagac catataatat tattatctta
    46381 aaaaaagggg acataataat atggttttga caggagcaca ttatatttac attctattta
    46441 tggtaattat attaattact atgattatga aaaaggatac aatagtacca tgtatattag
    46501 gtgttttttt tatgggattg ttctttgaaa aaaacatctt tggtgctata acagcagtgt
    46561 ttaactcttt tattatatct ttaaatgagt tgggtccaat tatcttgatt atagctatta
    46621 tggttgcatt atctaaagcc ctagaagcca ataatgctat acagtatatg gtgagaccat
    46681 tttcgagagt tataaaaaat tctaatacag cattttttgt aactggattt gttatgttag
    46741 ttctttcttg gtttttctgg ccaactccag ctgttgcctt agttggagct gtatttttac
    46801 ctgttgctat gagagctggg ctaccagcaa ttggtgttgc agtagcatta aatttatttg
    46861 gtcatggtct tgccttatct acagactttg ttattcaagg agctccaagt ataacagctg
    46921 gtgcagcagg tgttgcagta tcatatgtta taaatgatgg tatgattttg ttttgggtga
    46981 tgggaatcgt aaccatatct atggcatttt atacattaaa aagagatatt aacaaaggaa
    47041 tgtttagaga agaactaaaa ggttttgaaa gtgaagaagt agaagcgttc aatggaaaat
    47101 ctaaaatagc aactatttta gtagctttgg gatttcttgc tgatataatt gctatgtatg
    47161 tatttgacct aaaaggagga gatgcatcag ctcttttggg tggaacagca gtatttttaa
    47221 taataattat aaatgttata aattttggca aagatagtct tgaaaatgta tgtgagaata
    47281 taattgatgg ttttgtgttt gggataaaaa tatttggagc aattatacca atagcagcat
    47341 ttttctatat gggtgaagtt gcaccattga caggtgtatt tggaaaagta ttagcaccag
    47401 gttcacaagg tttattatca gatataggca tagctctttc tcaaacaata ccacttaata
    47461 aatttgccgt atcaggtata gaaactgtag tgggagcaat aactgggctt gatggttctg
    47521 gattttctgg gatttcatta gttggttcac ttgcctctgt atttggaact gctataaatg
    47581 caagtgttgg tgcattagct gcattaggac aaatcagtgg tatatgggta ggtggaggct
    47641 gtctagttcc ttggggtctt atttcagcag cggcaatttg tggggttagt ccaatagaat
    47701 tggcaaagcg aaactttgtc ccagttataa ccggattggt tgtaacaacc atagtagcaa
    47761 tgtttattat ttaataaatc gaataaatct ttaattgtat aaattaaaaa tagatgtgta
    47821 gctataattt attataattg ttttactaat tctaagccat acatctattt ttattgatat
    47881 ttttattagt attctttaat aaagtaatat ttttcattat attatacata acatctaaat
    47941 ataacatcta aaatactgtt atctatattt aatatcttgc caaatttttt gatgttttat
    48001 tgccctgtcc caccaacata tataagctat cacaagtttt tacctatact atcaccaaac
    48061 aaaaattcta attttaattt attattttat aacctatatt atattaacta tattagtttg
    48121 tagactttag atgtaattat aaatattaat actccaagag ttcaaattca tatcctagtt
    48181 ttcttgcatt tgatataaaa tcatttaaaa tttctgtact agttttggaa actgcatgta
    48241 gtaacaagat agaaccatca tgtagattat ccattatttt ttgcgttgct tcttcatgtg
    48301 atggttgttt atcagtatcc cagtctctgt atgcaaaact ccaaaataca gtcttgtatc
    48361 ctaaattttt agtcattgcc aagctttttt ctgagtattt tcccatagga ggtctaaaaa
    48421 atttaaccat atctttccca gttacatctt tatataactt ttcaacatca tataattcgt
    48481 cattaaagtt ttttagattg cttgtttttg ttggcataga tggatgggtt ttactatgat
    48541 ttccaactat atgtccctca gaaaccattc tttttactaa atctttattt tcttttatat
    48601 atggagctgt aacaaaaaaa actgctttaa cctggttttg ttttagagtg tcgagtattt
    48661 ttgtagtgta tccattttca taaccttcat caaatgtaag atatagtgtt ttttgaccat
    48721 cttttgtagg tccattgtat agggcatcat agtcagaaaa tttaaattca atgtccgtat
    48781 ttacttctgg tgttttatgc ttattgtttg gtatataaaa ccaatcaaga gtagtgttat
    48841 ctaaagaaga tacatctaat ttattattat cattattagt agtggctttt gatggtttat
    48901 ctgttccgtc tgaaactatt acattggaag tatctttacc actatctatt ttttccttat
    48961 cttcttgtaa ctgtgtttcc ttattttgat tttcgttttg attgttttgt gaatttgaac
    49021 atccaacaat aaaaaaaata gaaatagaaa gtagtgttaa ataatgcaat acttttctaa
    49081 acattttgaa gctcctttct aaattaataa taattgcata taataataat tatatgatta
    49141 attacaattg catatagtat tattataatt ggaaattact attaataata gttatatctt
    49201 taaaaataaa gtcacaatag tatgacaaag ggtgaataat caataaattt aaaaaaaaat
    49261 gaaaaaaata gttgaccata ttatgagaag atgttattat aataaatgtg ctcaacaaga
    49321 agtaacaaaa taacaagcac aaagaagtta attgaat
//
LOCUS       DAMJWU010000019        47029 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMJWU010000019 DAMJWU010000000
VERSION     DAMJWU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.019"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(102..1115)
                     /locus_tag="PC460_001235"
     CDS             complement(102..1115)
                     /locus_tag="PC460_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2600128.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLFLLYFFSNNLI
                     YAQNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYS
                     DFANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTS
                     EFGAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENT
                     DFGIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKH
                     PESKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIK
                     WML"
     gene            1343..2227
                     /locus_tag="PC460_001236"
     CDS             1343..2227
                     /locus_tag="PC460_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2600129.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ANKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            2429..3979
                     /locus_tag="PC460_001237"
     CDS             2429..3979
                     /locus_tag="PC460_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2600130.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            4008..4655
                     /locus_tag="PC460_001238"
     CDS             4008..4655
                     /locus_tag="PC460_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF2600131.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(4797..5057)
                     /locus_tag="PC460_001239"
     CDS             complement(4797..5057)
                     /locus_tag="PC460_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF2600132.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            5274..6557
                     /gene="hflX"
                     /locus_tag="PC460_001240"
     CDS             5274..6557
                     /gene="hflX"
                     /locus_tag="PC460_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2600133.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            6565..7242
                     /locus_tag="PC460_001241"
     CDS             6565..7242
                     /locus_tag="PC460_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF2600134.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDKAIKSLE
                     NAKNLKK"
     gene            7328..7771
                     /locus_tag="PC460_001242"
     CDS             7328..7771
                     /locus_tag="PC460_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2600135.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8076..8720
                     /locus_tag="PC460_001243"
     CDS             8076..8720
                     /locus_tag="PC460_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF2600136.1"
                     /translation="MSNYRTLHEFGMDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(8806..9183)
                     /locus_tag="PC460_001244"
     CDS             complement(8806..9183)
                     /locus_tag="PC460_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2600137.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(9183..9569)
                     /locus_tag="PC460_001245"
     CDS             complement(9183..9569)
                     /locus_tag="PC460_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2600138.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            9748..10503
                     /gene="nadE"
                     /locus_tag="PC460_001246"
     CDS             9748..10503
                     /gene="nadE"
                     /locus_tag="PC460_001246"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF2600139.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            10586..11305
                     /locus_tag="PC460_001247"
     CDS             10586..11305
                     /locus_tag="PC460_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2600140.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(11472..12476)
                     /locus_tag="PC460_001248"
     CDS             complement(11472..12476)
                     /locus_tag="PC460_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2600141.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            12742..13638
                     /locus_tag="PC460_001249"
     CDS             12742..13638
                     /locus_tag="PC460_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF2600142.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREMNLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            13776..15038
                     /locus_tag="PC460_001250"
     CDS             13776..15038
                     /locus_tag="PC460_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600143.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            15114..16307
                     /locus_tag="PC460_001251"
     CDS             15114..16307
                     /locus_tag="PC460_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2600144.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSIKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            16936..17730
                     /locus_tag="PC460_001252"
     CDS             16936..17730
                     /locus_tag="PC460_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2600145.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGVFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            17825..19165
                     /locus_tag="PC460_001253"
     CDS             17825..19165
                     /locus_tag="PC460_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2600146.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            19302..20534
                     /locus_tag="PC460_001254"
     CDS             19302..20534
                     /locus_tag="PC460_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2600147.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            20574..21716
                     /locus_tag="PC460_001255"
     CDS             20574..21716
                     /locus_tag="PC460_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2600148.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            21727..22521
                     /locus_tag="PC460_001256"
     CDS             21727..22521
                     /locus_tag="PC460_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2600149.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            22532..23554
                     /locus_tag="PC460_001257"
     CDS             22532..23554
                     /locus_tag="PC460_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2600150.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            23653..25029
                     /locus_tag="PC460_001258"
     CDS             23653..25029
                     /locus_tag="PC460_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2600151.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNRSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(25361..25705)
                     /locus_tag="PC460_001259"
     CDS             complement(25361..25705)
                     /locus_tag="PC460_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600152.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            26193..27203
                     /locus_tag="PC460_001260"
     CDS             26193..27203
                     /locus_tag="PC460_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2600153.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            27314..27802
                     /locus_tag="PC460_001261"
     CDS             27314..27802
                     /locus_tag="PC460_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2600154.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            27978..29906
                     /locus_tag="PC460_001262"
     CDS             27978..29906
                     /locus_tag="PC460_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2600155.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            30083..30493
                     /locus_tag="PC460_001263"
     CDS             30083..30493
                     /locus_tag="PC460_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF2600156.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            30515..30817
                     /locus_tag="PC460_001264"
     CDS             30515..30817
                     /locus_tag="PC460_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF2600157.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(31034..31726)
                     /locus_tag="PC460_001265"
     CDS             complement(31034..31726)
                     /locus_tag="PC460_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2600158.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(31752..32939)
                     /locus_tag="PC460_001266"
     CDS             complement(31752..32939)
                     /locus_tag="PC460_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2600159.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            33275..35152
                     /locus_tag="PC460_001267"
     CDS             33275..35152
                     /locus_tag="PC460_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2600160.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKVGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            35462..36868
                     /locus_tag="PC460_001268"
     CDS             35462..36868
                     /locus_tag="PC460_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2600161.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDIYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            36896..37744
                     /locus_tag="PC460_001269"
     CDS             36896..37744
                     /locus_tag="PC460_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2600162.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNEKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            38246..38938
                     /locus_tag="PC460_001270"
     CDS             38246..38938
                     /locus_tag="PC460_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600163.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            38935..39870
                     /locus_tag="PC460_001271"
     CDS             38935..39870
                     /locus_tag="PC460_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2600164.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            40018..40941
                     /locus_tag="PC460_001272"
     CDS             40018..40941
                     /locus_tag="PC460_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600165.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            40925..41695
                     /locus_tag="PC460_001273"
     CDS             40925..41695
                     /locus_tag="PC460_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600166.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TVLRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            41705..42487
                     /locus_tag="PC460_001274"
     CDS             41705..42487
                     /locus_tag="PC460_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600167.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            42864..43400
                     /locus_tag="PC460_001275"
     CDS             42864..43400
                     /locus_tag="PC460_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2600168.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            43432..43821
                     /locus_tag="PC460_001276"
     CDS             43432..43821
                     /locus_tag="PC460_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2600169.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            43858..44244
                     /locus_tag="PC460_001277"
     CDS             43858..44244
                     /locus_tag="PC460_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2600170.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            44371..45813
                     /locus_tag="PC460_001278"
     CDS             44371..45813
                     /locus_tag="PC460_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2600171.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            46110..46925
                     /locus_tag="PC460_001279"
     CDS             46110..46925
                     /locus_tag="PC460_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2600172.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        17588 a         5504 c         8717 g        15220 t
ORIGIN      
        1 ctaatatatt tggtctgtag gttaaataaa agtaccttta gaccaaatat attattaata
       61 acttaataac atcttaaaac ttatttattt atttatatta tttacaacat ccatttaata
      121 aatacaccat aacctacagc tgggtcgttt tcaattgcat gtgacactgc tccgattatt
      181 ttattatctt gaactattgg agtaccactc ataccttgta caataccacc agtttgagct
      241 aaaagctttt tatctgtaat tttaatttta aaacttttac tttctggatg tttttggttt
      301 tctatatcta taatttcaat attatatttt ttacacttat taaagttatc ttgtaaaatt
      361 atttgtgctt ttcctaattt aacttcatct aaagatgcta ctttatattt ttttaacttg
      421 gaaatatcaa agttattaat ttgacctctt attccaaaat cagtattttc tgtaaagcta
      481 ccaataatat tttctcgttt tgcatttata catccaacac tacctttata agatttttct
      541 atattaaggt gcttagttgt agaaattgaa cctgttttaa taggtatttt tcttgcatta
      601 ccaacactta ttggatgacc tacagctcca aactctgatg tatctggatt gatatacgtt
      661 aaagtagcaa atccagaaag taaattgtta aaactaattt tttctaatat gtgtttggta
      721 gttttcattg ttataagttg tccacctcta tttaataaaa tagatacttg tttttcttct
      781 ggtaaagcat ttaaggtatt ggcaaagtca ctatatccat caacttcagc attatttacc
      841 tttactatag catcacccaa tctaaatgga gatgcttctc caggatttct aactatgata
      901 ttttttaatt ctgcgtcaat ttgaagtacg tttccaattg gtattaaata ttcaggttca
      961 tttttttgta aattttgtgc ataaattaaa ttatttgaga aaaaatataa taaaaataaa
     1021 aataaaaatg ttaaaacaat tgtaaatttt gacttagcta agttggtttt tttacttaaa
     1081 gtattatttt taaaatgaaa atttttaagt tgcataaaac acctctttct tattaaatta
     1141 ttttaataat agaattgaag ttttcttgct gcttctatat tatgttgtgc aagaatcatg
     1201 ttttgatgct tggaaaacta gctcaataac accttagttt tcataaaaat aaataaaata
     1261 aatagtgtat atgataaaat aaatacatat tcatataaat aaaaataaac aataactgtc
     1321 tagcataaga aaggagcaaa aaatgaaaaa atatagattg ttagttactt gtataattgc
     1381 catgggaatc ataattatag gaacatcaaa tccagttagc aatttttaca agactacaaa
     1441 tttgattagt tcaggaaata tcacaaatga aaaagcaaat aaagatgaaa attcaaacaa
     1501 aaataataaa gataaaaatg aagacaaatc taattctaaa agtaataaag ataagtcatc
     1561 agaggacaaa tcgtcagata ataaaaatga aattcaaaaa aataaaaaac ttttaatttg
     1621 tatagaccca gggcatcaag gaaaaggaga tagcaattta gaaccagtag ctccaggttc
     1681 ttcctctaaa aaagcaagag tatcttcagg aacagagggt attgctacaa aaaagccaga
     1741 atatgtttta aacttagaag catctcttgt tttaaagagt atattagaat ctaaaggtta
     1801 taatgtaata atgactagag agacacatga tgtaaatata agtaattcag aaagagcaat
     1861 acttgctaat gacaaaaaag cagatatggt tgtaagaata catgcagata gtttaaataa
     1921 ttcttcaaaa actggagcat ctatattgat tcctgaaaaa gatggaaaat atacagctcc
     1981 tatatatgaa gaaagtaata aatgtgctga atttatcaaa cagaatatgg aacaatcagg
     2041 tatacaaatc aatggaatcg ttcaaagagg tgatttgact ggattcaact ggtcaaaagt
     2101 accagctgta ttagtagaaa tgggatttat gagtaactat aatgaagacc aaatgatgtc
     2161 aaatccagac taccaaagaa aaatgatgca gtgtatagct gatggattgg atgcgtattt
     2221 taaataagat atgaatagag aaaaagttag gttggtatta ttttcctaat ttttatctct
     2281 attttttatt gtacataaca caaagaagaa gtttatttta ggttaaattg tcgtaaatat
     2341 aactaaaaat attgggaata cttgatttaa gtttaaaaag aatatattat agtatagaat
     2401 gtgtaaaatt tttttgagag gtgttgaaat gctagtagta gaaaatgtga gtcatggatt
     2461 tggtgcgaga acaatattag aaaatgtatc ctttagactt agaaaaggtg aacatattgc
     2521 actagttggt gctaatggag aaggaaaatc gagtttttta aatataataa caaagaagct
     2581 tatgccagat gctggaaata taaaatggtc ttcaagagct acagtaggtt acttagacca
     2641 acatacggta ttaagcaagg gaaaaactat aagagaagtg cttagagagg cttttaagca
     2701 tatgtttgac ttagagcaag aaatgattgc tatgtatgac aaaatgggag aagctagtga
     2761 tgatgaaatg agtaagcttc ttgaagaaac agcagagata caaactatac ttgaaaatag
     2821 tggtttttat atgatagatg caaagataca agaagttgca aatggtcttg gtcttggaga
     2881 aattggtctt gataaagatg taactgactt aagtggaggg caaagaacaa aagtacttct
     2941 tactaaactt ctacttgaaa atccaactat tcttatattg gatgagccta caaactattt
     3001 agacgaagag catataacat ggcttactaa gtaccttcaa gagtatgaga atagttttgt
     3061 cttagtttct catgatattg aatttataaa taatacatgt aatgtaatat atcatatgga
     3121 aaatggagag ttaaatagat ataaaggaaa ttatgatgaa tttgtaagac ttaatgatat
     3181 aaagaaacgt caagaagaac aagcttatga taaacaagtt gaagaaagaa aaagactaga
     3241 ggattttgta gctagaaata aagctagagt agcaactaga ggaatggcta acagtagaca
     3301 aaagcaactt gataagatgg aaatattaga aagaccaaaa gaaaaaataa aacctacttt
     3361 tgcttttaaa gatgctagag cagctagtaa aataattttt gaaacagaaa atttagtttt
     3421 agggtatgat gaagcattga caaaaccact taatttccat ttagaaagag gaaaaaagat
     3481 tgccttaaaa ggaatgaatg gtattggaaa atctactcta ttaaaaactt tacttggaat
     3541 aataaaacca tttgaaggaa atgttaaatt aggtgactat ttggaagttg gatattttga
     3601 acaagagagt tcaagagaaa atagtaatac acctatggat gaagtttgga gtgaatttcc
     3661 agggcttaca aactttgaag ttagacaggc tcttgcaaaa tgtggactta caaatgaaca
     3721 tataacgagt caaatgagag tattaagtgg tggagaagct gctaaggtta gactttgcaa
     3781 agtaatgtta aaaaatataa atttcttagt attagatgag cctacaaatc atttagatgt
     3841 tgaggcaaaa gatgaattga agaaggctat caaagagttt aagggaacag ttcttttagt
     3901 atgtcatgag ccagaatttt attcagaaat tgttgatgat gtatggaata tagaagattt
     3961 tacaactaaa atcgtgtagt gatttaactt attgctgggg ggatttcatg aaaaagtttc
     4021 taaatgtact tttttcaatt tgtattagta ttgttataat agtaggtgtt attaatttta
     4081 ctgttggatt taaacaactt tattattttg acattgatta cttaaatata tcagagttat
     4141 ctggtttatc taaagatgat ataaaattga actacgatta tctaattgat tataacttaa
     4201 ataagaatgt gtctgagttc aaattaccaa cattaaaatc ttctccacaa gggaagatac
     4261 attttgaaga agtcaggaat atatttcaaa atataaataa attagctaaa ttattattgg
     4321 tagtttcttt agtaggaatt atattgagtg taaaaaataa aaatataaaa atcttaaaaa
     4381 ctacatcaat aacacttata attatgccat tattattaac agttccaatt ttattaaatt
     4441 ttgaaaagtc atttattatt tttcataaat tattatttag aaatgattat tggatattta
     4501 atcccgattt agacccagtt ataaatatat tgccagagga attttttttt cactctggaa
     4561 tgatgatttt aatattaatt ttgttagtaa gtatagtact gtttgtaatg tataaattat
     4621 gtaaatcttt atcaataaaa atatttcaat gttaaataga gggatatttt tatttccaaa
     4681 ataagataat aaaaaaggat tagattaaaa gttatacaat aaaaatagct gtgcaataat
     4741 atttagacta tgaaagtagt taaaaaatat ttatgcacag ctatttataa tagaatttac
     4801 tttttactag tgattttaac taaatcttga aatttactca taatgtaatt gtaattatct
     4861 tttttatgag gtaatataca actactgcaa tctttgatac ctttatctgt atacttaaag
     4921 tttccaccac agttttctcc taaagcatac aaaggacaat agcaaaatag acagttaaac
     4981 tcttctggtt tattagtttt atgacaaggg aaaaattcgc aatctttgtg attaaaaaac
     5041 ttataatttt cgcccatatt ttcctcctaa ttaaataaaa tttaaatttt ttatactata
     5101 taagattttg gtgataatat aattatacta aataatttta tgaatagtcg ataatatagt
     5161 attattaatc atagtattaa tggtataatt agtcaacaga aaaataatgt aaaaataatg
     5221 taagtgtatt ttgtaattag aatatgtttt ataacaaaaa atgaggtgat taagtgaaag
     5281 aaattcaaga aaaagcactt cttgtaggtc tgaatttgac tacaatagtg aaaaaaaatg
     5341 atgatataga cactaatgaa tctatggaag aattaaaaga actaacaaaa gctgctgggg
     5401 cagaagttgt aggtagctta attcaaaata aacactctgt agatgcagct tattatatag
     5461 gaaaaggtaa agtagaagaa ataagagcct atagtgactc tttagatgct actttagtaa
     5521 tttttaatga tgaattatct ggtgctcaaa tcagaaatat agagaatgtt gttggaagaa
     5581 aagtaataga tagaactact ttaattttag atatatttgc tcaaagggca cttagcaaag
     5641 aaggtaaatt acaagttgaa ttagcacaat taaagtatag attgccacga ctttatggaa
     5701 tgggtggtga gatgagtaga acaggtgcag ggattggtac tagaggtcct ggagaacaaa
     5761 aactagaaat agataagaga catatactta ataaagcagc agatataaga cgtgaactta
     5821 aagaagtaaa aaagaataga gagacacaga gagttaagcg tttaaagtca aatataccga
     5881 ttgtagcact tgttgggtat acaaatgcag gaaaatctac cctgttgaac gaattaataa
     5941 agacacacaa ggattatgaa caagaaaaag aagtttttgt aaaagatatg ctttttgcaa
     6001 ctttagatgt aaccttgaga aaggctcttt tgccgaataa aaaagaattt ttagtagtag
     6061 atactgttgg atttgtaagt aaacttcccc atgatttagt agaagcgttt aaggcaacat
     6121 tggaagaagt acagtatgca gatttaatct tacatgtaat tgatgcaaca aatactagtt
     6181 atgaattgca aaaaagtacg actgaaggtg tattaaaaga acttggtgta aatgataaaa
     6241 aacatatact tgtatataat aaagtggaca agctagaatt ggatatatat cctaaaagtc
     6301 aagaagatat tgtgtatatt tctgcaaaac aaggcataaa tatggataag ttattaaata
     6361 tgatagaaat tgctttaatg gaaaatactt attcagtaag tttgatgtta ccttatgaga
     6421 gaggtgatat atttagtaga ataaaagata aatataatgt ggagaatttt gagtatgggg
     6481 aaagtggtat aacgctagat gtagatttag atgaagaaga ttttaatatc tatagagagt
     6541 acatactaga aaaataggtt gaatatggat ataaactggg aaaatataga taatcttgaa
     6601 gattatttta taacctatct tctttataaa gaatctaaaa cagtatctca aattagtaaa
     6661 attagaaata tttcaagtac tgaagcaagt gagcaactta tacaagcaaa gttaaaaata
     6721 aaggaaatgc aaaaagatga ttttgaggca tctaaggata ttttggataa atttctagaa
     6781 ctagataaaa ttaagagact tgattttatg gatagcttag atgacgaaaa aatggtatac
     6841 tttaagagaa aggttttcaa aagaatcctt gtagaaaaaa atgctgaaga tttaattgtt
     6901 ttaatttggg cgacaggtga gctaaaagat gataggtttt tgaaattgct tcatcagctc
     6961 acaaaccaca gacactcaga tattagaaga ataacttatt cagcaatcag aaagattgaa
     7021 tcaccaagta gtagagaagt actgcaaaaa ggtctctacg acaaaaatgc acagactagg
     7081 cagtactgtg caaaagctct ttcaaaattg ggagatgaaa acagtttaaa gattttacag
     7141 cagttgaaag acaagaataa gaattttgaa aaagaatatg tgcttagagc ttatgacaaa
     7201 gccattaagt cattggaaaa tgcaaaaaat ttgaaaaagt aattttttat taaagcaatt
     7261 tgtttactta atgatgtatt tctaaagttt aacaaggtaa taaataaatt aatgggaggg
     7321 gtttcaaatg atagtaagac gttgtgcagg tggagtagtg ttttatgcta ataaagtact
     7381 tattgtaaaa aatgataggg gtgaatggac acttccaaaa ggaaagatac taggaggggg
     7441 acttccatat gagagtgctg ttcaaagagt taaagtagaa actggcatag atgctaaaat
     7501 gatagatgtg gctggcgata ctatgtatga atttttctca agaagtagac aacaagaagt
     7561 ttgtaatgca ataatgtggt atgttatgga agcttgtaac actgattgtg tacttgctcc
     7621 tgaatttcaa gaaggtggat tttataaagt aaaggatgct ttagaaatgt tatcacatca
     7681 taaagagcaa gctttagtag aagtatctta taaaaagttt aaggaattaa agaaattagt
     7741 ttcagagggc gatgatgttc cagcaaatta attgtgaatt tagaggattc tgaggaaggg
     7801 atagcccctt ccttttttaa ttgaagcagt tttaagcgta aattatatag tctctttaaa
     7861 ctatgattta tcaatttaga gatttagtag tttatattta aaatcagtaa gatatattta
     7921 aaataaaaat taaaaagtat acaattttat tatataattg tcttatgatg ttgattaaag
     7981 aattattaaa agtccattta tttttaaata gaataatttt ataaatatat atttgatgtg
     8041 tatattagaa aatgattaaa ataggaggaa atacaatgag caattacagg acattacatg
     8101 aatttggaat ggatgaaata acgattgaaa agtctgtttt tattggatat gctaagccta
     8161 taaagagtga agaagaagca ttagagttta taaatgaaat aaagaaaaag cataaagatg
     8221 caactcacaa tgtgtgggct tatactgtag gtaaaaatat gaatatacaa agatatagtg
     8281 atgatggaga accacaagga acagctggga tacctacatt agaggttata aaaaaagaag
     8341 atttgagaga tgtagcggtt gttgttacta ggtattttgg aggaacaaaa ttaggtgcag
     8401 gtggtctagt tagagcttat acaaaaggtg ctaaactggg acttgaagca ggtaagataa
     8461 tttataaggt aatgtatcaa gaagttaagg ttaagataga ttatactcaa cttggaaaag
     8521 tgcaaaatga gttaatgaat ttggggtatt ttataaaaga tactgtatat gaggataatg
     8581 ttgaaatagt agtatattca agattagaag atgtagagaa attatcagaa aaaatgattg
     8641 atataacaag tggaacagga aaaatagttt taggagagga attttatttg tctgaacaaa
     8701 atggagaaat tttaatataa tctaaaaggt tattagaata gttaatacct caaatagcca
     8761 attagtattt ttaagtgaaa agccattaaa ttgatttaat ctaaatcaat ttaatggctt
     8821 tttatcggat tttgcacaga atcctttttt tacttcaaat aaaccgacta aaagtaaaaa
     8881 tatcccaaat atttttttta atatatctga agacagataa ttagctaata gtgaaccaaa
     8941 tacagctcct aaaaccccaa acacagctat aggtataacc aatttaaaaa ttacattttt
     9001 attttttata tgtatagcaa gtgctataag tgtcataggg atagatgaaa gtaaatttac
     9061 gctttgtgct attttagtgt ctatgccagc aagtaaaacg agtgcaggta taaggattgt
     9121 tcctccaccc attcccattc ctccaataat tcctgcaaaa aatccaatta gagcaaataa
     9181 cattaaaaca ccatccttaa tgctgcaatt atcataatta ttccaaaaga aattctaaga
     9241 aactttccag taaccttatt tagtaattta gctcctataa taccaccaag aatacttcca
     9301 actgcaactt taattgttaa gttaacatca taagtacctt tagatacata taaaacagaa
     9361 cttgctgtcg ttaagaatat tattatagaa agagcagttg catgagattt atgttcttct
     9421 accttaacga tgtcatttaa tattggaact aaaagagtac ctcctccaga accaaaaact
     9481 ccatttataa atcctgtaaa aactccaatt atcgtatttt ttaagttaaa atcaaagtat
     9541 tttttcttta aatcatcagt ttttttcata aaaatcacct tctgaaatag ttttaccaaa
     9601 ataggcaaat tatatttata taaaataaga ttaaatataa tctttcatgt ttgtttaatt
     9661 ttgcataaaa aaaaacatat taaaacaaca tattaatata tggtataata tattaaaaca
     9721 aagtagcaaa tttgagagga gataacaatg tcaaatataa aaatacaaat agacaaaact
     9781 gtagaatggc ttataaataa ggttaatgag gcaaatgcaa aaggattaat tgttggtgta
     9841 tctggaggaa ttgattcggc agtagttgct aatttaatta aaaaggcatt tccagaaaac
     9901 tctatgggag ttataatgag cataaaaagt aatcctcaag atagagaaga tgcacttaag
     9961 gttatagagg gatgtgatat agaatacctt gatttagacc ttatagaacc acaaagtgct
    10021 atattagaca tggtagtagg aaatttaaaa gataaacatt tatatagaga agaatattta
    10081 aagatgacag atgcaaactt aagagcaaga gtaagaatga gtacaattta tacaattgct
    10141 aacaatctag gatatctggt tgttggtaca gacaatgctg cagaaataca cacaggatat
    10201 tttactaaat ttggtgatgg tggagttgat attttaccta ttgcaaattt aactaaagga
    10261 gaggtgtatg aatgggcaaa agagcttggt gttcatgaag atttaataaa caaagctcct
    10321 tcagcaggtc tttgggaagg tcaaacagat gaagatgaaa tgggaactac ttacaatatg
    10381 atagatgctg tgttagaagg tagattggat gaagttccta aaagagacca agagataatt
    10441 gaaagacttc atagattaag tgaacacaag agaaaaacgc ctgcacaacc tcctaagttc
    10501 taatttacta tttataagat ttgtgataaa atttacaagt gatgaaaatt agaataatta
    10561 attgtacgta ttaaggagtg gaaatatggg acgtataggt aatataatta atagaaaagg
    10621 taaacaagat gctcaaagag caaagatatt tactaaacat gctagagcaa tagcagttgc
    10681 agctaaggaa ggtggagcag acccagagta caatgcagcg ttaaaaacag ctatagaaaa
    10741 agctaaggca gataatatgc caaatgataa tatagataga gctatcgcta aaggtgctgg
    10801 agcaggtgct ggagaagact atgaaactat agtttatgaa ggatatggac ctggtggagt
    10861 tgctgttata gtagaaactt taactgacaa caaaaacaga actgctggta acgtaagata
    10921 ctattttgac aaaaacggag gaaacttagg aactagtgga tgtgtgtctt ttatgtttga
    10981 taaaaaagga caaatattag ttggtttagg agatggagtt tcagaagaag aattgatgga
    11041 tgttgcatta gaagcaggag ctgaagattt cataacagaa gaagatggtt atgaaataat
    11101 aactacacca gaagattttt ctagtgttcg tgatgagtta aaagctaaag gatatgagtt
    11161 tatatctgct gatgtgaaga tgatacctca aactacaact gtattgactg aagaatctca
    11221 tttaaaaatg atgaataaat tagttgatat gcttgaagaa gatgatgatg ttcaagatat
    11281 atatcataac tgggaagtag aataaatagt ataagcatat tgaaatttat aagtattgaa
    11341 tcaattttaa catgtgataa attttataga ataagaaaca ctaaaataga catacgcacc
    11401 tctaacctat atttgcataa taatatgtaa gcataggtaa ggggtgtttt tatatttata
    11461 ggttaagaaa tttaaagtaa attttgagct ggactcgatt gtctataaat aattgatggg
    11521 tatataactt gttgctgaat accatttata cgcccagtta ctgtatcaat aatcatccta
    11581 agactacgtt ctgctgtctc ttccattttt aaatctataa ctgtcaaagg aggaatactt
    11641 cttgccatta gtcgtgaagg tccattactt gctgcaaata tttgaatatc ctctccaatt
    11701 acaattcccg attcatgaaa tgcattcatc ataccaagag ctaccacatc atgattacaa
    11761 aaaattactt tttttagtga attttttgca agcatttttt gagcaatatc atatccacta
    11821 tctattttat tagaacaata atatatagaa tctgatagat gtattccatg ttctttacat
    11881 gtttttaaaa aagatttacc ccttaaagtc atacatgtaa aggaagttgg gttgaataca
    11941 acagcaatat catttttacc attttgaata gcatgtaaag ctgctatacg accagtttcc
    12001 tcattatcaa tagatacact tgaaaaacca ggtatttcac gatttacaac aactacagga
    12061 attttaggtt tattttttac tagatattct atatcatcag cattgatacc tgttaaaaca
    12121 attccatcat aataattatt tgcaaaaaag tattcatcat cacatagatg accaactttg
    12181 taaggtcgta tgatgatatt tatgtttaaa tgttctaaag aaattatgtg gtttattgct
    12241 tgaagtatat tggttgatgc caactcaaat ccctcatcct gccaataaaa agctatttta
    12301 atattattat gtttaatagg agtagagcgt ttatatccaa gcttatcagc tgaatttaat
    12361 ataagctctt gtgtatgtag cggaatcctg cgttcatttc ctttaccact taagacgtac
    12421 gagacagttg ctgaagaaag accacagtct tttgcaatat ctttgattgt tgccatgata
    12481 aaatcctcct taaatagttg cttaaatttg cttataccaa taatcttaac atatattcaa
    12541 ctaaagaaca atattctaca aggtgcatat tttacagaat tagaataaat aaatttaaaa
    12601 aaacttctta cttatgttga cataatttag tttataggat tataatatgc ttaaataagc
    12661 ttattattaa cttaaattaa gcaaagttaa gcaggaacat gcaatgctag cttgcaatgt
    12721 gactgtaaag ggggaaaagg tatgagtaag gtaaatatat ttgaagttgg tcctagagat
    12781 ggatttcaaa acctgaaaga gtttttagaa actgataaaa agcttaagat tattgatggt
    12841 cttattgctt cagggattaa acaacttgaa attagttcat ttgtaagccc aaaagcaata
    12901 cctcagatga aagatgcaaa agaagtagca acttattgtg tagaaaaata tcctgatgtg
    12961 aaactttatg cattagttcc aaatttatac ggtgctaaag ttgcatggga gtgtggtata
    13021 agagatatat cctatgttat atctttgagt gaaactcata ataaaaaaaa tataaatcgt
    13081 acccatgaag agtctattga tgagcttaaa aaaataatga atacttatcc agacatgaat
    13141 atatgtattg gaatggctac tgtatttgga tgtccttttg aaggtttacc aaaaattgaa
    13201 gatgccataa gatttgctaa acgcctttgg gaaaacggta tcagggagat gaatctagca
    13261 gatacaatag ggattgctga ccctaagcaa gtaagagaaa ctattagtgc attagttgaa
    13321 gctttaccag agtgcagatg gcaggtacat attcatgata ctagaaatat gggaatggtt
    13381 aatactcttg tggctattga atctggaatt actactgttc agtctacact tggaggatta
    13441 ggaggatgcc catttgctcc tggagcatct ggcaatactg caactgaaga tttagtatat
    13501 atgcttgaac gtatggggta tgatacagga gttgattttg ttaaacttct tgaattatca
    13561 aagtatcaga agagttttgt aaatggagta tatagtggtc atcatgtaaa tataaagtct
    13621 gatagttgta attgttagtt ctattgatta atcgattaaa aaaatatgtt gattaaaaaa
    13681 atatgctgat tggtaaaata gatttattga taaaaaatat aaattataaa atacattatc
    13741 taataaaaca aataaaaaaa ttgaggggga ttagtatgga tattatagct ttacttttat
    13801 ttttaggagt tattattatt gcatttgtac gtaagaataa tgcaggtatt ttggctctag
    13861 ctattggtgt catagctgtt agaatttttg gaatggatga taaagaactt tatggagcta
    13921 taagcaactc aatgtttact acacttgttg gtataacact actctttgca atagtgaact
    13981 caacaggagc attaaagctt gttgcagaaa aaattgtagc tgcttctggt aaacgtgttt
    14041 ggcttatacc tatagcagtt tttatagcag gatttgttgt tgctggtgtt ggacctgggg
    14101 caattccagc acttgcaatt atcccagcaa tggctgttcc tgtagccata acggttggtt
    14161 ataatcctgt aatgcttgca cttataggtg aatgtggatt aatggcaggt cgtatgactc
    14221 ctattactcc agaaggtcaa attataacaa ccgttgcaga aggggttgga attgaaaatg
    14281 ttatgcctac tatcttagcc tgtcaaactc tttctacaat tgtattttca atagtattgt
    14341 ttataatttt caaaggatat aaacttaaaa aacctataaa tgttatagat agtaagcaaa
    14401 tagaaaaatt caatataaag cagataattt ctctaataag catagtagtt atgattgctt
    14461 ttatcataat ttttgatatg aatgttgggc ttgcagcttt tgcagtttca gctgtactta
    14521 tttttttcaa tattgctgat gaaggagaat gtatcaaatc aattccatgg agcacgatag
    14581 taatggttct tggagtaggt gcaatgatga caatcgtaga tgcagcagga ggtattgact
    14641 tgatgagtaa tgcattatca tcactgatga atagtaaaac agctactcca attatgagta
    14701 tttcagcagg acttatgtct atggttagtt ctgctttagc agtagtttat ccaactatga
    14761 tgcctatgtg tgttgatata gctaaggcag ttggaggagt agacccacta gcacttattg
    14821 ctgcagttgg tgttggaggt tctcttgctg gtgtaagccc actttctact ggtggggcat
    14881 tgattttagc agctatgggt tcaagtaaga aagactttaa taaggaagaa caaaataagg
    14941 tttttgtaca gcttttcatt atgagtgcag ttggcttatt ggttattgca gtagtatcaa
    15001 tattatcatt taatgcaata acacatttaa tgaactaatt agaataactg aaaataaatc
    15061 attaaattta acttttaata ttaatcaata taatataatt tggaggggaa gttatgaaaa
    15121 aaggagcatt aagcaatata aaggtacttg accttacaag agttcttgca ggtccatatt
    15181 gtacaatgat gttggcggat tttggagctg atgttataaa aatagagatg ccaagaaaag
    15241 gtgatgatac aagaggtttt ggaccttttg taaatggaga gagtttatac tatgcaaata
    15301 taaatagaaa taaaaaaggt atcactctaa accttaaatc agaagaagga aaaaatatat
    15361 ttttagaaat ggtaaaacat gctgatgttg tagtagaaaa ttacagacca ggtgtaatgg
    15421 aaaagttagg tctgggttat gatgagttgg ctaaagttaa caatcaaata atttatggag
    15481 ctgtatcagg atttggatgt tatggaccat attcagaacg tccaggttat gatattattg
    15541 cacaggcaat gggtggtctt atgagtataa cagggcaaga aggaggagaa ccaacgagag
    15601 ttggtaatgc tatgggtgat gtactaggtg gaatgaattt aactattggt atactcacag
    15661 cactcaacgc tagaaaaata ataggaaaag gacaaagagt agatgtttcc ctcgttgatt
    15721 ctgttgtatc gtctctcgaa actggtatac agagatactt tgattcagga caacctgaac
    15781 ttatgggtaa tagatatgct tcagcctatc cttatgactc ttttaaggca agtgatggaa
    15841 gttttgtaat aggctgtggt aatgacaaac tttttgaaaa gttatgtaaa aaagttcttt
    15901 ttagagaaga ccttttaata gatgagagat ttaaaacaaa tcttaatcgt tgtgaacatt
    15961 acaaggctct taaaccagaa atagaaaaat ggtctattaa gcatacaata aaagaagcag
    16021 ttgacttaat atcagaggct ggagttcctg ctgctcctat ctataatctt gattgtgtta
    16081 caacagatga acatatagca aaagcaagag aaatgattgt agatattagt catcctatta
    16141 taggaaatat gaaagtcaat ggaaatccag ttaagttgat gacaacaaaa gctaaaattg
    16201 atatgccagc accatctcta ggggaaagta acgaggagat ttataatggt tggcttggtt
    16261 ttgatagtga taaaataaaa gatttaaaag aaaatggagt tatttgatta aaaaatagct
    16321 aaacacactt gtgactttag tcaggggagg ttcagttaga ataatatcaa aaaatattaa
    16381 atcaatactg taaatcaaat atagaattgt cagaagcatt taagatggga aaagctactt
    16441 agacattgag tctttgtggc ttttcctatt ttcattaaga attttatatt atcagaatag
    16501 atatgtacta aactaaagta ttaacaattt aaattaatag aaaataaact acctaattat
    16561 gttgaataaa gactacttta gtgaaaataa acttatagag atgctattta aattatttta
    16621 atggaaatat caaaaatgag actgcattct caaaaatgag actgcgtttt ttaagtattt
    16681 tctcgaaaat gagacaaaaa tattgtttta tgggttttat tgaagaaaaa ttcttctgtg
    16741 aattacaatt taatcaattt taaagttgaa aattattaaa aatttagtga aatttatgta
    16801 tatttcgttt attttattaa aaataacaat tttttactaa aaataaagaa cttcaatggt
    16861 gattggcata gtacttgcta ttttatatta acaagaaatg tagaaaaaat aacaatgtat
    16921 aatgggggga atatgatgga atctgtaatg gaaaatttga acaatcttaa ggtggaggtt
    16981 aaggacaaag tctgtgttat tactataaat agacctaaag ctttaaatgc tcttaatagt
    17041 gacactctta gggaactatc acaagttatt gatgtagtat cagaaaatga ggcaatatta
    17101 ggagttatta ttactggaga aggtaaagtt tttgtagccg gtgctgatat acgccagatg
    17161 caaaactata aaagtgagga aggcagaaaa tatgcaggtt atgctcaggg agtttttgat
    17221 aaaattgaag ctcttgaaaa aacagtaata gcagcagtta atggatatgc attaggtggt
    17281 ggatgtgaac ttgctatgag ttgtgatatt agaattgcat cagaaaaggc tatatttgga
    17341 caacctgaag ttaatttagg agttattcca tgttttggtg gaactcaaag attatcaaga
    17401 cttgttggaa ctggaatagc taaagagtta atttttacag ggagacaagt aaatgcagaa
    17461 gaagctaaat ctattgggct tattaataaa gtggttccaa gtgatttact tttagaagag
    17521 tctatgaaga tgatgaacca aatagttgaa aaagcgccaa tagcaataag atatgcaaaa
    17581 gttgttatta acaaagggat agatatggat ttaaagaatg ctcttgaact agagaaagat
    17641 attgcaggtt taacttttgc tacaagagat aagcaagaag gaatgaatgc atttatagaa
    17701 aagagaaagc cagtttttga aaataagtag ctattataac taaaattgga acaatgtaga
    17761 atatattcaa aatctaaatt tcgtgaaaaa atacatatag tttaatttta aaaggagtta
    17821 atttatgaat acgggagtaa ttataagttt agtagggatt atcatagctc tatctcttct
    17881 agttgtaatg gttatgaagg gaataaatat ttttattata gcaattgtat gttcatcatt
    17941 agttgctata acaggaagcc taaatgtgta tgatgcactt aaagtggatt atatgacagg
    18001 gtttgttgga tttcttcagg caaatttctt tattttccta acagggacat taatgggaaa
    18061 aataatggag attactggtg gagcaaaatc tattgctaag atgattgtaa gatggattgg
    18121 gaaggataaa gcactacttt ctattccaat agcttgtgga atattagcct atggtggagt
    18181 aagtgttttc gttgtaagtt ttgcagtgtt tccgatagct ttagaaattt ttaaagaagc
    18241 tgatttgcca cgaagattta taccagcagc tttgactttt ggatgctcaa cttttgctat
    18301 ggttgcacca ggggcaccac aaattcagaa tattgttcca gcatcaacat tagggacaga
    18361 tattatggct ggagcagtaa atggttttat atcatgtgca gtaatgttta tagttggaag
    18421 tacaattttg tacaaaatgg tttcaaaaga aaaagccaat ggtggtcatt ttatagcaca
    18481 tgaatcagat atatttgatg aagcagaagc aactaattct aagagagaaa atggtcccaa
    18541 tggtgtaatt gccttaattc cattgatagt gtcaatttta ataattaatg taaaagttaa
    18601 tggaaaagcg atagtaccaa ttgaggtggg agtatttatt ggagctttat tagtatattt
    18661 attgttaaat aaatatcaag ataatactaa aatagttgga catgttggtg atgcatgtaa
    18721 aactacagta gtagcaatat gtaatacttg tgctgtagtt gcatttggtt cagtagtaaa
    18781 atcagcagtt ggttttgact tcatagttaa tgctatgact aatattcctg gtcctcctat
    18841 tgcaggggct gcagttggaa ctacagttat tgcaggtatt tgtgggtcag cttcaggtgg
    18901 actaggaatt gcagctccac ttcttggacc agtattttta gccaagggtg tttccgctgc
    18961 agcattaact aggactatgg caatttcatc agcagcatta gattcacttc cacacaatgg
    19021 atatattgtt actgtaacaa atggtctttg taaagaaaca cataaggatg cctatatgcc
    19081 aatattctgg gtaactgttt taaccccatt tattggaacc atagtagcag tcctactgtt
    19141 tacaatgttc ccgggattgc cataagagaa aagataaaag gtataaatta tattttgatg
    19201 ttataaaatc aattttcaaa atatatacaa taaatttcat gttttaatac tgtaaatatg
    19261 tatttaaaat aacaaattga ccatataagg aggaattgaa agtgttaaat aaaccattag
    19321 agggagttaa ggttattgac ctaacgtatt ttgtagcagg tccagggact tctaaaatat
    19381 tagctgactg gggtgctgac gttattaagg tagagccaag ctttggagac ccaggaagaa
    19441 agacaggggc aacaatgaca atgccaatag atgattataa taatcctttt tacagcacat
    19501 ataattcaaa taaaagaggt ttgtctataa atcttaagag tgaaactgga attgaaatta
    19561 tggataagct tttaagcgaa gcaaacgtat ttgtttcaag ttatagaaca ggagcattaa
    19621 aaagactagg tcttgattat gaatcattaa gtaaaaagca tccacacctt atctggggtc
    19681 agataaatgg ttttggcgat tttggacctg ctaaggataa tgcaggattt gatacagttg
    19741 ccttttgggc aagaagtggg gctatgcttg atatagcaga aaaagataca agtccaataa
    19801 atccacctat agcctttgga gatgcaacaa cttcttgtag tttatctgga ggaatatgtg
    19861 cagctttgta ccagcaagct aagactggaa aaggacaaaa agtaatggca tctttatttg
    19921 ggcaagcaat atggaatgca tcatctttat tagcatctac acagtttagt gatgagtatc
    19981 caaaaactag aaaaaatgct atttcaccag taataaattc ttatcgttgt aaggatggaa
    20041 agtggatatt cttaagtatt ttagagcatg aaagatattt tgaggcactt tgtagattat
    20101 ttggaagaga agacttaata aaagaagaga aatttgcatc ttcagtagaa agtaagaaaa
    20161 atgctacaga acttattaat attttagatg gtgaatttgc aaagtttact caaaatgaga
    20221 tggttgaaaa acttactgct gctgatatag ctcatgaaaa aattcaacat gttaatgatg
    20281 tagtgactga tgaacaggct attgctaata actatatata tgaacataca agtaaagatg
    20341 gaaataagac aatgcttgca tctactccag ttaaatttgg aaatattgaa gttaacatga
    20401 cttgtgatgc tccacttata ggagaacatt cagatgagat attgagagaa cttggataca
    20461 gtgattctga tatagaggct ctaattgaat ctggaattgt aacaatgaat aaagaagcat
    20521 gtgaaatatg ttaataaaca tgtgaagtat gttaataaat aggagggaat tttatggatt
    20581 ttagattaac agaagcacaa ttaatgcttc aaagagtagc aaaagaattt gcagagaatg
    20641 aaatagcacc aatagctgct gaaacagata aaacaggtat ttttccaagg gagcttttta
    20701 gcaaaatggc taaaatagga tttaatggaa ttggaacacc tgtagagtat agtggttctg
    20761 gtggggcaga tatagaaaag gtaattgttg ttactgagat tgccaaaaaa tgtgcagcat
    20821 ctgcagcaat attatcaata cacacaatat atgctcaagc tattttgaaa tttggtactg
    20881 aggaacaaaa gaaaaaatac cttccaatga tggctgaagg tgggtgtgta ggtgcttttg
    20941 ccttaaccga acctaatgct ggttcagatg ctgcgagagc tgcaacaact gcaattattg
    21001 atgaagaaac tgatgaatat gttttaaatg gaactaagtg ttttatttct ggtggaggtc
    21061 aagcagaatc attaataata tttgcattga cagacccatc aaagggaata aagggaatgt
    21121 ctgctatcat tgtcgacaaa ggtacgccag gtttttcaat aggaaaaatt gaagaaaaaa
    21181 tgggtattca tggttcagaa actgcagagt taatatttga taactgtaga gttccaaagt
    21241 ctaacttatt aggaaaagaa ggtaaaggat ttaatatagc tatgacttgt ttagatggag
    21301 caagaattgg agttggagct caagctgtag gaattgctga aggggcttta gaagaaagta
    21361 tcaaatattc taaagaaaga gtccagtttg gcaagccaat ttcagctttg caaggtattc
    21421 aatggtatat agcagatatg gcgacaatgg tggaatcagc aaaattatta gtatattatg
    21481 ctgcagattt gaaagctaga ggagaaaaac acactaaaga agctgctatg gcaaaataca
    21541 atgcgtcacg tactgcaaga gaggttacaa atttagccct tcaaatccat ggtggatatg
    21601 gatatatgaa ggattatcca ttagagagaa tgtacagaga tgccaaaata acagaaattt
    21661 acgaaggtac ttcagaaata cacaaagttg ttatttcaag agcagtacta ggataggagg
    21721 aaaagaatga atatagttgt ttgcttaaag caagttccag atactaatga agttaaaata
    21781 aataaagaga caggtactct aataagagat ggtgtaccaa gtataatcaa tccagatgat
    21841 agaaatgctt tagaagaagc tctaaaaatg aaagatgaac taggagcagt gattaaggtt
    21901 ataagcatgg gacctcctca agctaaaagt gctttaaaag aagctttggc aatgggagct
    21961 gatgaagctt atttaatctc agatagagct tttggtggtt cagatacttg ggctacatct
    22021 acaattattg ctgctgctat agagaaggtt ggaaagtatg atgtaatatt ctgcggaaga
    22081 caagctatag atggagatac agctcaggtt ggaccagagg ttgcagaatt tttagggata
    22141 ccacaagtta cttatgcgaa agaagtaaag gttcaagatg acaaattact agtaactcgt
    22201 tatacagaga caggggatta tttaatagaa gcaaagatgc cagttttatt aactgctata
    22261 aaagagttaa acaatccaag atatccaagt gtcaagggaa tcttagaagc ttataataat
    22321 ggagatgcaa agattactgt attaacactt gcagatttag atgttgatac aactcaaatt
    22381 ggattaaagg gttcacctac aaacgtttat aagtcttttg taccagttaa agacaagcat
    22441 aatgaaataa tagaaggtat aaataaaaaa gaaaaagctg agaaactaat agaaatacta
    22501 ttcgatttga aactagtata ggagtgttaa gatgatgaga gcaaaagtta atcaaggtat
    22561 aaatttaaat gattataatg gtgtttgggt aataggagag caaagggaag gtaaaattaa
    22621 tcctgttacg atagaactta ttggtgaagg aagaaagctt gctgaccaat taggaaaaga
    22681 gcttgcagtt gtgatagctg gatatgaagt agagaaagaa gtgaaggaat tacttcacta
    22741 tagtgttgat aaagtatatt acatcaatga ccctcttctt aaagatttca ctactgatgg
    22801 atatgcaatt tcaattgcaa atttaatcga aagaaaaaag ccagaggttg tattggttgg
    22861 tgctacttct ataggaagag atatagcgcc tagaattgca ggtaaggttg gaacaggtct
    22921 aactgctgac tgtacaaaac ttgaaataga ttcaacagat aataagttat tacaaacaag
    22981 accagcattt ggtggaaatt taatggccac tattgtttgt ccaaaaaata gaccacagat
    23041 gtcaacagtt cgtccaggag ttatggcaaa agctgtaaga aacgaatcag agactggaat
    23101 tttagaagtt gttactccag aacttactga aaaaatgatt agaactagat tagtagagat
    23161 acttccacaa gaaaagaaat ctgtaaatct aactgatgct agaataatag tatcaggagg
    23221 aagaggatta aaaagagcag aaggatttga gcttataaaa gaattggctg ataaattggg
    23281 ggcagagatt ggtgcttcaa gagcagcagt agattcaggg tggatagaac attctcatca
    23341 agtgggacaa acaggaacta ctgttagacc agaactatac attgcatgtg gaatatctgg
    23401 agcaatacaa catttagcag gtatgagtga ttctaaatat atagttgcta taaataaaga
    23461 tgcaaaagcc cctattttta gtatatgtga ctatggaata gtaggagatt tatatgaaat
    23521 tatacctgaa atgatagaat cattaaatag gtaactttta gaaaacattg aatatgtaga
    23581 atgataagtc ttataatatt gtattgtaag acttattatt tttgctgaag gttatagtgg
    23641 ggagatgaaa caatgaaaga gcagtggtat aaggatatat ttgcaagagt attatctatg
    23701 actgatgatg gttttattgt tgtaaataga agtggagtaa taatagatat taatgataaa
    23761 tattgtgact ttttaggaaa agaaagaaaa gatataatag gacaaaatat acaaagtatt
    23821 ataccaaata caaaaatgct agatgttatg aaaaataagt attgtgaaga aggagctata
    23881 catcattatt caggtggaaa tacaaaagaa aaaagtgtaa tagtaagtag gtcttatgtg
    23941 gaaaatgaca atggagaagt agttgcagga gtagctcaag ttaaatttag attgcaatca
    24001 tttgatgtag ctaaaaagtt aatgtcagaa tatatggagc tccaatatta taaagagcag
    24061 tttaaggata actgtggttt tgacaagctc ataggtgaaa atagagactt catagaactt
    24121 aagaaaacag gtgtgaaagc ttctaaaaca aactttccag tacttttaac gggtgaaaca
    24181 ggtacaggaa aagaagtctt tgctagagca attcacaata atagcagtag gtcagataaa
    24241 cctatggtta gtataaactg cgctgcaata ccagaagaat tattagagtc agagctattt
    24301 ggctatgacg aaggagcttt tacaggagca aaaaagggtg gtaaaaaagg taaattttta
    24361 gttgcgaata atggtacaat ctttttagac gagataggtg acatgccttt gaccatgcaa
    24421 gcaaaacttt taagagttct tcaggaaagt gagatagaac cagtgggagg tcttaaaact
    24481 ataaagatag atgtaagagt aatctctgcc acaagaaaga atttaagtaa aatggttgaa
    24541 gagggattat ttagagaaga cctatactat agattaaatg taataaacat tcatatgatg
    24601 gaattgaagg acaggcaaga tgatatttta ttgctagcaa attatatatt aaacaaatta
    24661 aatgttgaat acaaggaatt gaaggtttta agtgataagg ttaaaaattg ttttattaac
    24721 tatacttggc cgggaaatat aagagaacta caaaatgtaa taaaaagtgc ctatgctgta
    24781 agtgatgata tggtcattat gatgtgtgac cttccatcta agatggataa tatcagcaga
    24841 gtagctcaat gtaatgtgga tagcaattgt tcaattcatg aaatggttga gaattatgaa
    24901 aagagcttaa ttattgatgt gttaaggaag tataattgga aatgttctaa ggcagcagag
    24961 gttatgggaa tacacaagag tcttttatat aagaaaataa aaaagtatga aattgaatta
    25021 aataattaag ataaattgaa ttaaggcaat gctaaaaaaa cgcccttatt caaagcgaac
    25081 tgtataaact gttttatgca gttaaaacgt gtaaaagtag atgtgaagta ctaatgtcaa
    25141 tcattagtac ttttttatta tatttataat tactatctat atttcagtat cttttgatat
    25201 ctaagattat tatgtttaat ttaggatata acaaaaccct cttagttatg gacagttttt
    25261 tatattttaa ctgtctacta agaggggata gtttagttta aatttagagt aaattttatc
    25321 tagcatttat aaaaaatatt ttgaatagct tttttgttat ttaatcataa ttttctactt
    25381 ctttatcaag ttctttgcca caagaatcac acttatatat agcattgtat ccgtaatcat
    25441 attcatcaac tattttcttt tgtctaaata caccaccaca ctcacatagg aaaccaattt
    25501 ttataagtgt aacgataaat tgtgatttta taaattttat atcattacat ttatcgttag
    25561 tgtaaaatgt ataaatataa gagttatcgc actcatctct taattcaaat ctttcaatat
    25621 atgtgttatt ttttaattcc tcaaattctt ctttagttaa atatgatttt tgttgcaatt
    25681 catgtaaacg cttaatttgg tccattattt atatcctccc aataatttta gtattttaag
    25741 cttagataag gaatctaagt tttattaaat ttatcccaag gaattattta aatcatattt
    25801 agttttatta attacaacat atataaatta cacgaaaata aaactaattg taaaaataat
    25861 atcctgtgaa aacctaaact actaacccct tactaacccg aaaactaaaa aaagaaaata
    25921 tatccaaata taagtgatat ataaataaag atgtttcata taaatttaac tttaaatatt
    25981 ctgacaataa tatatataaa tatattatag ttaaaatata ccacatattt tgggtattgt
    26041 atactggaat tagagtaatt aatttatttt ttaacataca aatgatttta tgaaaaaaaa
    26101 caagactcaa aaatttttat attctttgat agaataaaat tgagagtaaa tttattgaat
    26161 tttataaaat ttataagggg tgagtctaag atatgcatga agataaaaat gatatatttg
    26221 aatcaataga tactgaggat tttgataata aaacagaaga agaatgttta gagctagtaa
    26281 gaaaaaatgg attgaattta atatacataa aaaatcaaac agaagagata tgtctagagg
    26341 ctgttagaca aaattgtaat gcaataaaat gtgttcagaa tcagacagaa aaaatatgta
    26401 tagaagcagt taaacaagat tggagaatgt tagaatttgt agaagagcca acagaagaaa
    26461 tttgcatgtt ggctattaac caagatggaa cagcattaaa atatatagaa aatcagacag
    26521 aggagttatg cttaagagcc gttgaaaaaa atggtgcagc tttggagtat gtaaaagaac
    26581 aaacagaaga aatatgtata gaagcagtta gaaacagcga atttggattg gcaagagtga
    26641 aaaatcaaac agaaaaaata tgtatggaag cagttaagca ttgtagctat aatctaaaat
    26701 gggtagaaaa tcaaacagaa gaaatatgta tggaagcagt gagacaaaat gggcttgatt
    26761 tgaaatttgt aaaaaatcaa acagaaacaa tctgtttaag agcagtaaga caaaatggaa
    26821 tggcattaga atttgtaaag gaacagactg ttggtatatg cttaaaggca gtaagacaat
    26881 atggaatggc attaaaattt gtaaaagaac aaacagaaga aatttgtaca gaagctataa
    26941 aacaagataa aagagcactt agttttgtaa aaggagataa ggaaaaatac aaggctctat
    27001 atgataataa tgaaccattt gcaaagagat atgttagaaa tgtaatagaa aaagaaaaca
    27061 gagcattgat taaaaaagga gaagaaaatg caaaaatcaa aattgcagga aaactttatg
    27121 atagaggcat gtcttttgaa gatatatcag atatagttga gattgaaata agcaagttaa
    27181 aagtgagttt aggtgttttt taagggaaat agttaataaa aaaataatcg actagctctt
    27241 tgtaggacag caaaattagt gatataattt gatgtataat aaattatttt tattagataa
    27301 gggggaatta aagttgaaga ttacagtatt ataccaaaca agaagtggaa acacagaaag
    27361 ggtcgctaag ctgatagaag agggcgctaa aaaggtggat ggagtagatg taaagcttat
    27421 gagacttgat aatattgatt tagattattt aaatgaaagt aaagcagtaa tatttggaac
    27481 acctacttat ttagcaaata caacttggga agtaaaaaaa tggtttgatg aagattcaaa
    27541 aaaagttaat ttagctggaa aattaggagc tgtttttgct acatgtgatt atatatgtgg
    27601 aggtccagat gttgcgatac ttactatagt aggacatttg atggttaaag ggatgcttgt
    27661 gtattctgga ggtggttctt taggaaagcc atttatacat ttaggacatg tacattcaat
    27721 tgaaggtcca gagttacaag atgagaaggc tataatattt ggtgaaagaa ttgctactaa
    27781 ggcaaaagaa ctatttgctt aatttctata tgcataataa tgattagagt aaatttaggg
    27841 ttttaatgga gatttgaagt aaaagttata aaataaaaaa ttttccataa aattactcaa
    27901 gatgaattat aatataatta taaataaatt atattattaa aatatttaaa atcaaacgtc
    27961 aatagcgagg gaagtttatg gtatggcata tagaatatga gattttttct gcaattatag
    28021 ttattttttt aatggtatac ttttttagaa gtaaatttat acctacttta caaaacaaaa
    28081 tatattgtgc acttttaata ttctcatttt tatttataat ttcaaatatt ttaggttcat
    28141 tttgtctaaa taatatagac aaaattccaa tttttattac ttttttattg aatcagatat
    28201 acttattgtt attgccaata ccagcagcac ttgtatcatt ttatgttatg gctattattt
    28261 atcaagatat aaggtatatg aaaaaaagat tattattttt atctatacca cttataattt
    28321 gtataatctt atctattaca aatcctttta cgcatattct atttggattg tcaaaagaaa
    28381 caggttatat acgaggttgt ggatatgttg caacttttgc atcttttttc ttttatgcaa
    28441 tatattcagc atttttatct tttaaatata gaaaagcaat gcatgaatca aaaatatggg
    28501 ctattagagg ttttcttata gtttcaattg tggcaattat cttacaggca atttttttac
    28561 agtatcttct tacaggaact gcatgtgcat gtagtttact tttgacatat ctttctatgc
    28621 agaatcgagg tcttattatt gatgacctta ctggtgtact caatagacaa tcttttattc
    28681 aagagctaga tttgaatatc aatactgaac aatcaggatt tattattaca attgcgcttg
    28741 atgattttaa gtttatgaat gaaacttttg gaacaaaaaa tggagatatt gcactaaaag
    28801 aagttggaaa atatttgatt cagatacatg atgtaaacca tgtatataga atagggggag
    28861 atatattttc tattgttgtc aatagtcaat taggtataga accagatgat attatcagaa
    28921 agatagaaga aagatttaaa aagccttggc tagtagaaga gataagtttt aatttagcaa
    28981 ctagtattgc tgttgtttat tacccagaaa atgcagaaac gacagaagat gtaattgttg
    29041 ctatagattt ctctatacat gaagcaaaac gttctgagtc aaggcgagtt gtttatgcag
    29101 atgcgtctat aagtgaaaag ataaagaaga aacatgttat taaaaattgt ttaaaagaag
    29161 cattaaaaaa tgatgggttt gaagtgtact accaacctat attttctaat agcaggggca
    29221 ggtttacttc agcagaggca ttacttaggc tagaacacaa agagttaggt tttatacctc
    29281 ctgatgaatt tattccaatt gcagaacaaa caggattgat taactcaatt ggattagtag
    29341 tctttgagaa ggtatgtaga tttatagcta gtgatgagtt tgaagattta aaattggata
    29401 atatagctgt gaatctttct gtagtacaat gtatgcaaaa aaacttagct gatgatttac
    29461 tttatcttat gaataaatat aatgtatcac caactaaatt taaacttgaa attactgaga
    29521 ctgtggctat gggctctttt aatataatta aagaaacaat ggaaagatta attgatttag
    29581 gtgttaagtt tgcacttgat gattttggaa ttgcttattc tggagttact aatatgcttt
    29641 ctttaccatt ttcagtaatt aagttagata agagtttgat ttggtctatg aatgaagata
    29701 gtaggcataa acttacagtt gagacgatta tagctcttat aaataaattg aatatgaagg
    29761 ctgtagcaga aggtgttgaa accattaagt gtgctgaaga tttaatagct atggagtgtg
    29821 aatatttgca agggtattat ttttctaaac cagttccaga agatgttttt aaaaatttgt
    29881 tgaaagaaaa tacttttaca agttaacacc ctttattttt ttaattacaa ctttgttcat
    29941 tgacactgcc agcgatatat tataaaatta acacaataat aaatattaaa tttattatac
    30001 atattattaa tattaattaa tggagtaaag atttattata tattttattt aattatccta
    30061 aagtgagaaa ggaagatgtg acatgaataa aaagaaaata ttagtgccaa ttgatgggac
    30121 agaaagaagt atgcattctt tggaatttat aaaaggaata tttaaaaaag atgaagttga
    30181 agtagaaata atgaatgtaa aagaactagt atttattgat ggcatatccc tggctgaaga
    30241 gataaaaaat tctgaaaatt taggaagaag aatcttggat aaagcagcag agataatggg
    30301 agagtatgat gttaaagtac attttacttt tggatatcca ggagatgaga taattagaaa
    30361 agcaaaagaa gataatgtag attttatagt tatgacaaaa tctactaaaa aaggtcttac
    30421 aagaatgata ggttcagtaa cagcaagtgt agttaaacaa gcaaaatgta tagtgatgat
    30481 tgtaccggag tagaattaga aaggtatttg aaatatgaat aaaaagatag tagcagtttg
    30541 tgaaagtgaa agaagtttaa attctcttaa gacagctgca aatgatttag gatttgattt
    30601 agtttatgaa attcaagaaa atgataatat aaaaaatgaa ctttctattt atgatataga
    30661 agatgcatcc atagtgttat ttgttacaag tgattctata gaaagcatag agaagattga
    30721 tagatttata gatagagaat tttatgaagt tgatccaaag tatataatag aggatgcaaa
    30781 atctatagta agtgaaattt taatagactt aaattaatcg tgggtgtcgc actaagaata
    30841 gagcgacgcc tttttatata ttttacacaa gaaataaaga ggttgtatca aaatatagat
    30901 aagagtcttt ttcattgttg aaatgatgaa aatatatttg atttcataca aaaaagagta
    30961 cctcataact aaaaagttca ctttgagaca ctccattttt atatgttagt tgcatttaag
    31021 gtaaaataga agtttaatca tgttgaaata actgaattac atcttctaaa tgattgtaca
    31081 ttgatgtata acctaagtaa gagtctttac tatcaaatgc aatcattatc ttgttatggt
    31141 ggctaatcat atccttgaga gaatttctat atttaaaacc aaagtattgg ttagaagaaa
    31201 ctgttttagg tattaactca taatatttta taaaaattgg attatgagaa gctaaaatta
    31261 tctctttgtg aaattgcaag tcagcatcat aacattcttc tatcaaaccg tgagaaacct
    31321 tatccaattc ttctattgac tttaggtaag tatattttat tttttgaagt tctttagatg
    31381 tagcatttaa aattgctagt ttagtagctt cactttcgat aagttgtctt gctcttaaag
    31441 attctacata aaattcttgt atgttatgat ttttatagat ttcaccatca tcaaataggt
    31501 caactttttt aaatccttca actataggac ttgaataagg cacagctcta agtattcctt
    31561 ccttacagag tatatttatg gcatttctta caggtattct acttatgtta aataggtcag
    31621 caatctttct ctcacttggt aatttactat taattttaaa agttccattt agaatgttta
    31681 atttaatata atcaataatt ttttcagtgg catttaagct tttcataaaa actcctaaac
    31741 atataatttt tttatgattg tattatatca tagttaagag ttaaatctac ttttttataa
    31801 tatgtataag acaggataga ggccaaaata gctataatag ccaagataaa gtatccatat
    31861 tgtattgaag tatattccga tattttacca actatcattg gaaatacaga cattcctata
    31921 gcataaattg cttgaaaaaa acccattgca gtagatttct gatgttttgg tacatttttc
    31981 atagactcgc tagtaaggta agaaaggagt atacctgtag atatacctgg tagtacttgt
    32041 agtacaaata ttatattaat attttttatt gttggaacta aaaagcaata ggctgaaact
    32101 aaaataaaaa ctaaaggaat ccaaaatttt ggcccatact tagtacaaaa tttagtagaa
    32161 gcaaagccag aagaagcaac agcacacacc atgtaaaata tagaagatag acctatgagg
    32221 gtagaactag aacctaagtt tttaagaatt tgagtagtaa atgacattgt tgtagacatt
    32281 tgaataccct gttgaattaa tgccataaag gaaaaaaata caagtctttt atccttgcag
    32341 atagataaat attttttaat tgatattttt tcaggtattt tttttgaatt atgatttttt
    32401 atatttaagg ctagaatcgt tccaataact gaagacaata cactcatgat acataaggct
    32461 gtcattccta atttttcata aaataaagta ctcataacaa atccaatcaa cattcctaga
    32521 ttgtttacta atattattgt acttgtagct ttttgttgat gttctgtaga aaaaaagttg
    32581 gtatagtgaa ccatgtaaga aatccaagtt gatgatgata ttccagataa aatgttagct
    32641 ataagaaaac cattgcctcc aggagaaaag actcttataa gagatgctaa accagcaaag
    32701 aaaattccag acagtataaa tattttatgt ttatttatgg aatcagcaaa tactccaaca
    32761 ggaagcctta ataaacattg tgagattcca tatgcaccta ttattatacc aatcatattt
    32821 gtagatacat tacatgcact taaatatgga ttttggaaag gtatatatac atattgtgaa
    32881 aaccagaaaa atgtaacaat agaaagtaaa agtaattttt gattagatat ttttttcata
    32941 ttatcctccg tatggtattt ttataatgca aataatatac catttggatt ttttaatcaa
    33001 gtactttctc tattaaaaaa aatttaaata tataattatt atttacaaaa aaattatact
    33061 ttatagttga attaagaaaa cgcttatgat actataaagt taacaaattt gaatatataa
    33121 cagtagacgt gttactggta atgcaggcaa gatcgaatca atttttatag attatctata
    33181 gagtactatg ggtataatct atttatttgg ttcgattttt ttgtttaatt ggccataaaa
    33241 caaaaagcct atagttgaaa atggaggatt ttttatggat tataaaaata tagctcaaga
    33301 aattctacta aatgtaggag gaaaagagaa tgtaaatgaa gtgactcatt gtatgacaag
    33361 acttagattt aaagtcaaaa gcgcttctaa agtgaataag gataaattat ctaaaactga
    33421 aggagtaata actgttgttg agagtatggg tcaaatacaa gttgtaattg gaaataaagt
    33481 aaaaaaagta tatgatgagg ttataaaaat tgtacctcaa tcaaataacg taggtaaaaa
    33541 gaatgaaagt caagaaaagc aaggtataat taattctata ttatcagcag tagctggtat
    33601 atttacacct acaatacctg ctatagcagg ggttggtatg attaaaggta ttttatcagt
    33661 tcttgctatg tattatatga ataaaaatgg agtagatata aaagaaacgc aaagctatat
    33721 tatcttaaat gccatggctg attctatatt ctattttatg cctattatac taggatatac
    33781 tgcggctaaa gtatttaatg caaataaaat aatatctatg gtattaggag ctacactttg
    33841 ttatccaact tttactgctt tgatggcagg tgaagaatct gttagatttt taggattggc
    33901 agtaacaaaa gctacgtata catcatctgt tattccaata attattgcta tatgggctct
    33961 ttcatacgtt gaaaaagtat tagaaaagta tatacctgag gtaattaaaa taataatggt
    34021 tccaacttta tcattggtag taatgttacc tgcaacatta tttttatttg gaccaatcgg
    34081 aatttatata ggaaatgtca taaacttttc ttataagtat atttatgaat taagcccagc
    34141 attgtgtgga gcatttgtag gtgggttatg gtgtgtgttg gttatatttg gtgctcatag
    34201 agcattatta ccaataggta taagtgatgt tgctcaaaca ggaagacaga atttattggc
    34261 atttgcagga gctgcaaact tttcacaagc aggggctgct ctaggagtat tctttaaaac
    34321 taaaaatcaa ggactaaaaa caatttctat gtctgcaact ataacagctt tatttggtat
    34381 aactgaacct gcaatatacg gtgccaatct tagattaaaa aagcctatgg tatgtgccgt
    34441 aatatgtggt gcatttggag gtgcgataat gggaatgggt ggagcttatg gtaatgcatt
    34501 tgcaaaccaa ggtgttttaa ctatacctgt atatgctgaa gcaggtgcat taggtttctt
    34561 aagttatcta ggtggttgtg ctatagcatt ttttggttca gctatttcaa cttatctagt
    34621 tggatttgaa gatttggaag agtcttctaa taaggaaaat tcatctataa aagtagaaac
    34681 gaaggatggt gttattgata taacttcacc tgtagagggc gaatgtatag aattgtcaga
    34741 agtaaaggat gatgtatttg cttcaaaagc tatgggagaa ggtatagctg tgttacctac
    34801 aaaaggagta ataacagccc ctactgattg tgaagtagct tctttatttc caacattaca
    34861 tgcaatagga ttaaaattag ataatggagc agaaatgtta atacatgttg gaatcaatac
    34921 agtagagtta aatggtaaat actttaccaa acatgttaat caaggagatt tagtgaaaaa
    34981 aggtgataaa cttatatcgt ttgatataga taaaataaaa aaggttggtt atgatgtaac
    35041 tacgccagtt atagtaaaca atacatttga ttttgggcaa gtagtttctt gtaaatctag
    35101 ttatgtttca accaatgata atataatttc attagtatta gctggtaatt aagttaattt
    35161 gactagagat aagtaaattg atttaaaagg aaataagtaa atcaacaatt aaatacatct
    35221 aaaagtaagt ttattgcaat gaattgttaa agaaaataat taatatggag taaatctatt
    35281 gcaatgaagt aacaaagaaa ataattaata tagagtaagt ttattgtaat gaattgttaa
    35341 agaaaataat taatataggg taaatctatt tcaatgaaat aacaaagaaa agagttaata
    35401 tagagtgaac ttattgcaat gaatcaacaa agaaaataat taataaaaag gagaaatatt
    35461 tatgaataca ggatttccaa aagacttttt atggggagca tcatcttctg catttcaagt
    35521 ggaaggagct tgggataaag acaataaagg aaaaaccgta gccgactata attcttttaa
    35581 aaagtctcat ttacaggcag atactaaagt cgcatcagac ttttaccata attatgaaga
    35641 agatattgaa ttaatgaaag aattagggat gaaaacatat cgtttttcaa tttcatgggc
    35701 aaggataata ccagatggtg aaggagagat aaaccagaaa ggtttagact tttataataa
    35761 gataatagac aaactaatag agtgtgatat tgagccattc gtgactttat accattttga
    35821 ccttccattt aaattagtag agaaatataa tggttgggaa agtagagaaa ctgtctatgc
    35881 ttttgaaaga tttgctaaaa tatgctttaa gcattttgga gatagggtaa agtattggca
    35941 acctcataat gaacagaatt taattgttag agtggaggag agaattaata tatatgatga
    36001 aacagattca tggaagatag ataaaatgcg tgctcagatg gattataatc tttgcttagc
    36061 acatgcatta gcagtcaatg catgtcatga gatgataaaa gaaagtaaaa ttggagcagc
    36121 agtatcatca tctgtaacat atcctcttac ttctaagcca gaagatatat atgcagctag
    36181 aatgaatgat aattttaaag tgtactatat gcttgatatg catcattatg gagaatatcc
    36241 agggtattat atgaagtatc ttgaaaaaag aaatatagtt cctcacatgg aagatgggga
    36301 taaagagata cttaaaaaag ctaaaatgga ttttatagct gtaaattatt ataggacaaa
    36361 ttgtgcagaa gctttacctg aagattcaca acatccattt ggattaagag aaggaactgt
    36421 tgattttagt atgtatggac tatttaaaat gtcaatgaat cctaacttag aggcttctga
    36481 gtatggagca gcaatagacc catcaggtct tagagttgcc ttaaatgaat attggcaaag
    36541 atatcactta cctgttataa taacagaaaa tggtctagga gctaaagata tattagaaga
    36601 tggaaaaatt catgatgact atcgtataga ctatttaaga agtcatataa atgcatgtaa
    36661 actagctatt gaagatggtg tagagatgat tggatattgt ccatggtcat ttacagacct
    36721 tttaagttct tctcaaggtt ttaataagcg ttatggactt gtttatataa atagaactga
    36781 ccatgaaatt ttagatttaa aaagaatcaa gaaagatagt ttttactggt ataaagaagt
    36841 tatagagaac aatggaatag taaaataaac ataaatatat attgagggtt ttagtatgta
    36901 cattacaaag gtattaaata attcgttgct acttgcaaag gatgataatg atgaagaaat
    36961 aatattgatg gggaaaggca ttggccataa ttataaggcc gggtctgagt taaatgaaaa
    37021 agatatagaa aagatttttg ttcttcatga tgaaaatata aaaaagagta ttatacaatt
    37081 ggcaacagaa atagatgcag aatattttgg tatagctcaa atgataattg catatggaat
    37141 agaaaaatat aacttaaaac ttatgaacca tatatatttg gccttaactg accacattgc
    37201 ctttgcagtg agacgtttta aagaaggtat aatgattgaa aatcactatt tgttcgaaat
    37261 caaggaattt aatccaaaag agtatgatat aggaaaatat gctattactg tttttaagga
    37321 ggttcttggt ttagacctac cagaagaaga gattggtaat atagcaggcc attttataaa
    37381 tgcacaacaa gataatcctt atagtgatag aaataagaga agtgctaaaa ttgtaaatgc
    37441 tattttgcaa atagttcatt atcacttttc tattgtatat aataaagagt cattttatta
    37501 taggagattt gtaatgcatc taaaggcctt ttcacaaaga tttctggcta atgaaccatc
    37561 aagagaaaaa atagatttta tatatgagca agttcaaaaa aattgtaagg aagagtatga
    37621 atgtgtgaaa aaggtaggag cttatatgta taaggaatac tccagagaat taccaagaca
    37681 agaggagtta tatttgatga ttcatattca taagatttta ggagaattat gcgaaaatag
    37741 ttagaatatc ttaatatgct agtaatatta taaaaaatta aaaattaact gcactttaaa
    37801 tatgaataat tgcattactt aagggggttg tcttaaaatt gattttttta attaaatttg
    37861 aagctcaact ccttttttaa acctttatat cattacaact ttaatgttta tacgcctgta
    37921 cttcagatgt atatctactt tttacatttc tcaacatgca aatctaccat tctgtatata
    37981 gtaaatgtat tatcatgttt gttttattca agcacactta taatacgctt gattttttca
    38041 caatgatgaa atatactaat gggtgttata ggtataagaa tcgcattaca cttgaagaag
    38101 taagtgataa atgagatata aatcctcata tgataaacta ctatttcatt gaggaacata
    38161 ttctaagtga tgtcaagata ctacaatatg actaaattcc tagtgataaa aaagtctacg
    38221 aatgagtgta gaaaggggaa ctaagatgaa taaagtttta attatagatg atgataaaga
    38281 tctttgtatt ttgttaaaaa agagtatttc aattgaacag attgaagcaa actattgcca
    38341 ttctgggaga gatggactgg ttgaactttc aaaacaagat tatcagttag tagttctaga
    38401 tgtgatgatg ccaggtatgg atggttttga aactatggaa caaatacgaa aatatagcag
    38461 tattcctatc ttaatgctga catcaaaaag tgataattac agcaaagtgc atggattgcg
    38521 agctggtgca gatgactatc taaccaaacc atttgaaatt acagaattta ctgctcgtgt
    38581 actgtcttta atccgacgtt acacccgttt taatccaaaa aatgaggagc cacagcaact
    38641 ttctttcaaa ggtttgttga ttgaccttga ccagcataca gttactacca atggcgaacc
    38701 tatgttgctt ccacccaagg aatttggtgt acttctttac tgtgcaaggc atcagggtaa
    38761 gattttgaca aaacagcaga tttatgaagc cgtgtggggt gagccatatg tatatgatga
    38821 cagcaatatc atggctatta tcagtcgcct aagaaaaaaa attgaactag acccatcatc
    38881 tcccatctat ttgcaaacaa taaaaggaat tgggtatcaa ttcaacacgg aggtatgaaa
    38941 atggatgaac aaacgataat tcttttctta ctcttgctgt cgattggtct actttttctt
    39001 tccatatttg ctttttccaa gcttgggcat attcacaaac gcttaaaaga tatagaggag
    39061 atactcgctg atgtacaaat tggagacgaa aatcgtaaaa tactaattaa gccatgtgat
    39121 gttatggctc cattggttta tcagcttaat gagattgtct acgattatga aaataagtta
    39181 ctttctctga aaaagtcgga taaagctagc aaacaactta tgacaagttt atctcatgat
    39241 gttcgtacac cattaacaac tctgattggt tatctggatg ctgtacacag tggtatagtt
    39301 atcgaacaag agcgggagga ataccttgaa atcgctaggc gtagagccta cgacctcaag
    39361 gattacattg atgttctttt tgactggttt cggttgaact ctgatgaatt tacgctgtca
    39421 atagaatctg tggaaattgc agagttgtca cgcaatattt tgaaagactg gattcctata
    39481 ttccatgaaa agaaactgaa ttttgaaatt gatattccag aaaatcgact aatggtaaac
    39541 ctcgacccag atggatattc ccgtgtggtc aataatctgg ttcaaaatgt tcttgctcac
    39601 agtaaggctc ggcaaatcaa gattaccatg agtgaggatt ccagaatggt attactccgt
    39661 gttgaggatg atggtgttgg gattgccaga gaaaatttac cacatatttt tgagcgactg
    39721 tataagtgtg ataaaggacg ttcagaaaaa ggaagtggtc ttggtctatc tattgtcagt
    39781 caaatggtgg agcgaatggg tggtcaggtt tccgtagaaa gtgagattgg aaagtatacc
    39841 gtatttacag tgagcttacc attaatataa aattgttttt cccccatgtg acatgtatac
    39901 acagggggat tttttgtaag ttataaagtt gcaaggttaa tacaaggttg ttgcaaggtt
    39961 aatgtaagat tgaggtggta tgataacttt aataaacaaa ggaggctttt attatttatg
    40021 aataatttaa ttattgaaac aaaaaatctc actaagcaat atggtgctca aaagagcgtt
    40081 tctaatttga atattcacgt tcagaaaggt cgcatttacg gattgctagg aagaaacggt
    40141 gcgggtaaga cgacaactat gaaaatgtta ttgggcctaa caaaaccaac tactggagaa
    40201 attaaaatat ttggtcagga cattcacaat aacgagaaga agcttcttcc tcgaattgga
    40261 tgtttgattg aatcaccagg gttttatcct aatctgacag gaacagaaaa tctcaaaatc
    40321 tttgcaaggc ttagaggaat accaaaaaaa aatgcagtag aatcagcact ggatgtagtc
    40381 ggtttgccaa acaatgacaa aaagttattt tctcaatact ctcttggtat gaaacagagg
    40441 ttggcgattg ctcttgccat tatgcatgac ccacaactgt tgattttgga cgagcctatc
    40501 aatggacttg atcctattgg tattgcagaa gtaagaacgt ttattcgtga tctctgcaca
    40561 gaacgaggta aaactatttt gatttccagt cacattcttt ctgaaattgc cttgttggca
    40621 gatgacattg gcattattga ccatggtatt ttgttggagg aagaaagtct tgcccaatta
    40681 gaagaaaaaa acagtaaata catacatttt gctgtatctg atgcagcaca ggcagcaagg
    40741 cttctggaaa cccaacttaa cctgaaaaat ttcaaagtag cagataatta caatattatg
    40801 ctatatgata cagatgtttc tgttgcagaa ataaatcgtg ctttcattct aaacaacatt
    40861 gaggtatcag aatctcattt atgcgaagat actcttgaag attacttcaa gaaagtaaca
    40921 gggggtgaag gaattgcttg atataatttc atgtgaattt tcaaaactca agcggttaaa
    40981 atttatactg atttctattt taggtgcttg cttatttcca attcccgcaa caattttgat
    41041 tgcaaaggac aacttgccct ttgagcaatt gttcaaatta gttgtcaatt ttggatattt
    41101 cttgctatta ccaattgtat taagtattgt tgctagccaa ctatttttta tagaaagaga
    41161 taatgatgta ttaaaaaatc ttgcaacagt gccagtgcca aaaggaaaac tggctctggc
    41221 aaaattggct gtgttgctgt ttatatcatt gttctactct gttgcagggt taggagctac
    41281 tatcattgga ggtttcattg ttggaatagt agagggggtt gctgtaaaat taggaatgag
    41341 cattgctttt ggaattatgt tttttgttac tgtacttcct gttgtggtac tcattgtata
    41401 tttcaatcgc agctatattt tttctattat actttctttt gtatatgcta ttttcaattt
    41461 tagtatatcc ttaaatatta tcaattttga accaaataac ccactattaa gcgtattacc
    41521 tgctcctgtt ataatgcgtt ggtggatggc attttgggga gatcccactg gcgaatacac
    41581 ggtacttaga cagccatatt tgctctcaac tcctgcttgt gtaggtattt tatgtttgat
    41641 aaccattata gcagtcctgc tgatttgtat gatatacaaa aagcaggaga attaggaggt
    41701 atctatgctt gatataatta ctacagaatt tcaaaaaata aaaagatata atattctatg
    41761 gattggtgtt gttgctgttc tgttttcagc actattggca gtttttcagg ttgtaagttc
    41821 acacggcagt gaccctctaa cgtatgagag ctttgcaaac ggagttatct ggaataattt
    41881 cagtcttagt tttccatttg ggattacact tattggagga tatcttatta accgtgaata
    41941 tacagaccaa accttaaaaa acatcctaac tgttcctatt tcattacgca aattgctagt
    42001 gggaaaatta attgcggttg gaggaattac tgtattgtta agtcttttca gcttcttatg
    42061 cacatttatt ttggggttgt tattttgcaa tatcaatata tctgttatac tcgttgtaaa
    42121 atccttagtt cagattttgt ctgttaatct gtattgttat gttgctgttt tgccgattat
    42181 tgcttatttt ggtcgtaaac aaaatgcatt tctgacgggt gtagggattg catttgtata
    42241 cggtttttgt ggtgtgtttg ttgctggaag aaatctgaca gacttttatc caatcacagc
    42301 aggtcttggt ctagttggat acaacaatgg tgctggtact gtttatcagc cacttatcgg
    42361 tgctatgact ttgattgtaa ttctaatctt gacaactatt ctacttgtat ttacacctaa
    42421 ttatgataaa gttatgacca tatctaagaa aaaagggata aagcataaaa agtattctca
    42481 taaataacat aaatattgtt tctattattt aagttgctag acagtgaaaa gaaaaaatct
    42541 gtcgtacagt ggctatatgc tcatgtattc aagaagctac agttttgatt tttcataact
    42601 gtagcttctt tttgtattta ttaaatcacg actatcataa tacttgcaat aggtatagta
    42661 acaagagaga gtactgtcat atgtgtttta tatattacat taagtatatt ctaaaatata
    42721 tgttttaaat aatatatttg aaaataggaa tatttaaata aaaaaaataa tattatatat
    42781 agttgacaaa aattggcaaa tgatatacta tataaatata gaaatagtaa tcattactat
    42841 tattaaaaag ggaggaatta attatgaact taaaaggaac taaaacagaa aagaatttaa
    42901 tggcaggatt tgcaggagag tcagaagcaa gaaataaata tacatactac gcttcaaaag
    42961 caagaaaaga aggttataat caaatagctg ctatatttga agaaactgct aacaatgaaa
    43021 aggaacatgc taaattatgg tttaaacttt tacatgatgg tatgccttca acagaggaaa
    43081 atttaaagga tgcagcagca ggtgaaaact atgaatggac tgatatgtat gcaaaatttg
    43141 ctaaagaagc tagagaagaa ggatttgaca agatagctta tttatttgaa gctgttggaa
    43201 aaatagagaa agagcatgaa gaaagatact taaagctttt agaaaactta aatgaaggaa
    43261 aaatatttaa aagagatgaa gaagttgttt ggcaatgtca aaactgtggt catgtgtatg
    43321 taggtactga agcaccagaa aaatgcccag tatgtgacca tccaaaagca tattttaata
    43381 taaaagctga aaattattaa aaattatcat tattaaaaag gaatgttaac tatgaaattt
    43441 tctaaacaac gagaactgat tttaaatgaa atattaaata atccggttca tcctactgcg
    43501 gattacctat atgaaaactt aaaaaaagat aatccgaatt taagcttagg aactgtgtat
    43561 agaaatttag ctcagctaac ggaacatggc tttataagaa aggttagtat tccagggtat
    43621 ccagatagat ttgatggtcg aatagataat cactatcata tcatatgtga agtatgtgga
    43681 gaggtatacg atttagaatc cgaagttctt aataatttac aagaattaat atctgaggag
    43741 acagacataa aaataacatc ttataacata agttttaaag ggatttgtaa taattgtaaa
    43801 aggtgtagcc aagtaggtta gttttcaaat tctaaaaata attaatgagg tgagtttatg
    43861 tgtagtgaac aaaaattttt tatatgtgag acttgtggaa accttgtagg aatgatacaa
    43921 agtggtggtg tacctatttt ctgttgtgga aaacctatga aggaacttgt tcctaataca
    43981 acagatgcag cagttgaaaa acatgtacct gtaattgaag tggatggaaa taatgttact
    44041 gttaaagtaa gtagcacaac tcatccaatg actaaagaac atcatatagc atgggtgtat
    44101 cttatgactg aacaaggtgg tcaacgcaaa tgtctagcag tagatggaga acctgtagtt
    44161 aaatttgctt tgaatgatga tgataaagtg atttctgcct atgcttactg taatttacat
    44221 ggattatgga aggctgaact gtaatattta aataaattga gtcaaaaagg aggctataaa
    44281 tgtattttca tacaaatagc ctcctataaa tttatataaa ttcaagtttt aattttgttt
    44341 aatgtcaata taatataagg agaaaataat atgtcaattt ataaatgtag tgtttgtgga
    44401 tatatttatg atgaatctaa gaatgataaa acatgggatg aattaagtga agattgggaa
    44461 tgccctgtat gtacaaaagg tcgtagttat tttggaaaga taagtactgt ttactatgaa
    44521 gaagatgaaa agatagcaga agacatagtt gaagatgaat ctaaattaaa tactgaaaaa
    44581 gagggagatt taaattactt aagtacatat ttaagaagag atgatgaagt tgagaaacat
    44641 atggatataa ttcacgaaat ggctgttaca ggaaaatcta taatagaacc tatgagaact
    44701 aaactaccag taatttcttg ggatgatatt ttaattatgg gtgctcaatt aaatccatta
    44761 cctttaaatg agcatgatga agtaaataca acaacaataa taggaaaaaa agcaaagaaa
    44821 cctatgatta tagaaaatcc agtttatatt tcccacatgt catttggagc tttgtctaaa
    44881 gaactaaaaa tagctttagc aaagggtgca gcccaaaata agacagctat gtgtagtggt
    44941 gaaggaggta tattaccaga agagaaggaa gcttcataca agtatatttt tgagtatgta
    45001 ccaaataaat atagtgtaac tgaagaaaat cttaaaaatt ctgatgctat agagataaaa
    45061 attggtcaag gaacaaaacc tggtatggga ggtcatcttc ctggcgaaaa agtaactgaa
    45121 gaaatagcaa aagtaagaaa taagcctgta ggtcaagatg taataagtcc atcttgtttt
    45181 gaggaaatac aatccaaaga ggatttaaag aaacttgtag atgaacttag agaagtttca
    45241 gaagggcgtc caataggtgt taaaatttca gcaggtcata ttgaaaaaga tatggaattt
    45301 atagcttatg ctaaacctga ttttgtaact attgatggtc gtggtggagc tactggagca
    45361 agtccaaagt tgcttaaaga tgcaacttca attccaacaa tatttgctct ttatcgtgct
    45421 agaaaataca tagatacaca tggattagat atagatttgg ttataacagg tgggcttcgt
    45481 atatctactg attttgctaa ggcaattgct atgggggctg atgcagttgc tattgcaagt
    45541 tctgctctta tggcagcggc gtgtcaacaa tatcgtatat gtggttctgg taagtgtcct
    45601 gtaggagtag ctactcagga tgaggaattg agaaaaagat tacatataga aaatagtgct
    45661 aatagagtgg caaacttttt aaatgtaagt ttagaagaac ttaagacttt tgctagaatt
    45721 tctggtcata aagatataca tgatttaagt gttgatgatt tatatactgt taactctgaa
    45781 atatcaaatt atacaaacat acagcatgta tagattgaag tttgaaacta ttagatttta
    45841 gacagctata aaagtaaatg ataaatatat tttataaaag agagtgtctt aaaatgaact
    45901 ttttagttca taagtcactc ttttttgtat gaagccaaat atatttatca ttttagcaat
    45961 gaaaaagaat cttatctctt tattttgaga tagactcctt ctatttatga aaattaaaga
    46021 tattaaattg taaaatattc tgtgatgtcg tactattaaa ctaaagattc tgtactttaa
    46081 agtaaataaa aatttaagga gtgattatta tggaaagatt gaagatacaa gttttagtcg
    46141 acaataatac ttatattgat agatactttg ttggagaacc agcagtaagc tattatattg
    46201 agattgatgg aaataggatt ttgtttgact cagggtattc agatgtattc atatcaaacg
    46261 ctgaaaaatt aaatatagat ttaggaaatt taactcatgt tgttttttct catgggcata
    46321 atgaccatac aagaggtatc cagtttttag aaaataggta tgatttatct acagttgaac
    46381 ttatttcaca tcctaactgt tttataccta gaaaacatgg agagaaaagc ataggagctc
    46441 cattttctgc tgaggaaatt aaaaatattt ttatgtataa tcctaaggat aagccattta
    46501 atctaagcaa gaattgtgtg tttttaggtg aaataccttc tataaatgat tttgaaaaaa
    46561 gagctaaaat tggtaaatgt aaaataggag atttgtggga agatgattat gttttagatg
    46621 attctgctat agtttgtaaa actgataaag gtatttttat tgttacagga tgttctcata
    46681 gtggaatttg taatatagta gagtatgcta aaaaggtatg tggagatgat agagttattg
    46741 gtatactagg tggttttcat ctttttgaat tgaataatag gttagatagt acaatacaat
    46801 atttggataa agaagaaata agaatgttgt acccttgtca ctgtgtttct tttaaggtaa
    46861 aagcgaagat gaatgaaata ttgaatataa atgaagttgg agtagggctg accataagta
    46921 tatagtattt gaaagtatat aagatttaag ttgaaggtta aaaagatatt ttctatagaa
    46981 aatatctttt ttgttacgtt tattttaaga gaaatcaata tacttttat
//
LOCUS       DAMJWU010000020        46622 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMJWU010000020 DAMJWU010000000
VERSION     DAMJWU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.020"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(115..894)
                     /gene="hisJ"
                     /locus_tag="PC460_001280"
     CDS             complement(115..894)
                     /gene="hisJ"
                     /locus_tag="PC460_001280"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF2600173.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLILGSDSHCAEEINKYDIV"
     gene            complement(1440..1790)
                     /locus_tag="PC460_001281"
     CDS             complement(1440..1790)
                     /locus_tag="PC460_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF2600174.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(1806..2222)
                     /locus_tag="PC460_001282"
     CDS             complement(1806..2222)
                     /locus_tag="PC460_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF2600175.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(2241..2432)
                     /locus_tag="PC460_001283"
     CDS             complement(2241..2432)
                     /locus_tag="PC460_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF2600176.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(2583..3398)
                     /locus_tag="PC460_001284"
     CDS             complement(2583..3398)
                     /locus_tag="PC460_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF2600177.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(3445..4383)
                     /locus_tag="PC460_001285"
     CDS             complement(3445..4383)
                     /locus_tag="PC460_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2600178.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(4380..5252)
                     /locus_tag="PC460_001286"
     CDS             complement(4380..5252)
                     /locus_tag="PC460_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2600179.1"
                     /translation="MNKNLSEKMEIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(5277..7205)
                     /locus_tag="PC460_001287"
     CDS             complement(5277..7205)
                     /locus_tag="PC460_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF2600180.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(7317..7694)
                     /gene="gcvH"
                     /locus_tag="PC460_001288"
     CDS             complement(7317..7694)
                     /gene="gcvH"
                     /locus_tag="PC460_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF2600181.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(7781..9907)
                     /gene="cdhC"
                     /locus_tag="PC460_001289"
     CDS             complement(7781..9907)
                     /gene="cdhC"
                     /locus_tag="PC460_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF2600182.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(9964..10770)
                     /locus_tag="PC460_001290"
     CDS             complement(9964..10770)
                     /locus_tag="PC460_001290"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2600183.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(10820..12187)
                     /locus_tag="PC460_001291"
     CDS             complement(10820..12187)
                     /locus_tag="PC460_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF2600184.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(12218..13162)
                     /locus_tag="PC460_001292"
     CDS             complement(12218..13162)
                     /locus_tag="PC460_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF2600185.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(13182..13952)
                     /locus_tag="PC460_001293"
     CDS             complement(13182..13952)
                     /locus_tag="PC460_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2600186.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14008..15393)
                     /gene="lpdA"
                     /locus_tag="PC460_001294"
     CDS             complement(14008..15393)
                     /gene="lpdA"
                     /locus_tag="PC460_001294"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2600187.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(15437..16318)
                     /locus_tag="PC460_001295"
     CDS             complement(15437..16318)
                     /locus_tag="PC460_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF2600188.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(16354..17028)
                     /locus_tag="PC460_001296"
     CDS             complement(16354..17028)
                     /locus_tag="PC460_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF2600189.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(17065..17937)
                     /locus_tag="PC460_001297"
     CDS             complement(17065..17937)
                     /locus_tag="PC460_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF2600190.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18029..18661)
                     /locus_tag="PC460_001298"
     CDS             complement(18029..18661)
                     /locus_tag="PC460_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF2600191.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(18766..20442)
                     /locus_tag="PC460_001299"
     CDS             complement(18766..20442)
                     /locus_tag="PC460_001299"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF2600192.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(20691..21470)
                     /locus_tag="PC460_001300"
     CDS             complement(20691..21470)
                     /locus_tag="PC460_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2600193.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(21780..23699)
                     /gene="cooS"
                     /locus_tag="PC460_001301"
     CDS             complement(21780..23699)
                     /gene="cooS"
                     /locus_tag="PC460_001301"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2600194.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(24306..25217)
                     /locus_tag="PC460_001302"
     CDS             complement(24306..25217)
                     /locus_tag="PC460_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2600195.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(25333..25911)
                     /locus_tag="PC460_001303"
     CDS             complement(25333..25911)
                     /locus_tag="PC460_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2600196.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRAIDEIPE"
     gene            26137..27060
                     /locus_tag="PC460_001304"
     CDS             26137..27060
                     /locus_tag="PC460_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2600197.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(27232..28374)
                     /locus_tag="PC460_001305"
     CDS             complement(27232..28374)
                     /locus_tag="PC460_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2600198.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(28392..30146)
                     /locus_tag="PC460_001306"
     CDS             complement(28392..30146)
                     /locus_tag="PC460_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF2600199.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            30282..30488
                     /locus_tag="PC460_001307"
     CDS             30282..30488
                     /locus_tag="PC460_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600200.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(30493..31416)
                     /locus_tag="PC460_001308"
     CDS             complement(30493..31416)
                     /locus_tag="PC460_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600201.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(31670..33370)
                     /locus_tag="PC460_001309"
     CDS             complement(31670..33370)
                     /locus_tag="PC460_001309"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF2600202.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(33780..34193)
                     /locus_tag="PC460_001310"
     CDS             complement(33780..34193)
                     /locus_tag="PC460_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2600203.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(34259..36637)
                     /locus_tag="PC460_001311"
     CDS             complement(34259..36637)
                     /locus_tag="PC460_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2600204.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            36956..38407
                     /locus_tag="PC460_001312"
     CDS             36956..38407
                     /locus_tag="PC460_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF2600205.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(38611..41124)
                     /locus_tag="PC460_001313"
     CDS             complement(38611..41124)
                     /locus_tag="PC460_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2600206.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTSQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKALDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(41347..42168)
                     /locus_tag="PC460_001314"
     CDS             complement(41347..42168)
                     /locus_tag="PC460_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2600207.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(42686..43162)
                     /locus_tag="PC460_001315"
     CDS             complement(42686..43162)
                     /locus_tag="PC460_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF2600208.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(43197..43775)
                     /locus_tag="PC460_001316"
     CDS             complement(43197..43775)
                     /locus_tag="PC460_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2600209.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGNEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(43879..46479)
                     /locus_tag="PC460_001317"
     CDS             complement(43879..46479)
                     /locus_tag="PC460_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2600210.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHSNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNVFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        14114 a         9027 c         5235 g        18246 t
ORIGIN      
        1 cttattatta ttttgtttaa gttctattac tattttcaat catataaatt tacaaaagac
       61 tgtacataaa tttattttat acaaacttat ctacagtctt ttgtgtgaat atatctaaac
      121 aatatcgtat ttatttattt cctctgcaca gtgagaatca gaacctaaaa ttaatggaat
      181 atcgtatttt tttaccaact ctaataagta tccatctata taaggctctt tgcagtactc
      241 ttttctattt ccagaaacat tataatcaag ttcataacct ttttctttaa ttaactttat
      301 taactcttca agaattgtat ttcctttata atcgtatgga aatacctgat taaactttct
      361 aactaaattt aaatgaccta ttctttttgg tttatgtata cctaaatctg aatttacagc
      421 ttttatcaat gtttcataat acttattata aacagcttca atacttccta atttgtcgat
      481 taaattttga aactcctcta cactgtaatc tacacaataa taatcatcat caatcttaat
      541 tatatgtact gataaaagag aatcctctaa ttcctttcca tatttattta aattctcctt
      601 tatcccttct tcgtatcctt ctataaaatc cacttccgac ccaacattta tcttgatttt
      661 accctcatac ttttctttca acttcttgac atcattaaaa tactttggta aatcttcaat
      721 actcatacaa ctatcatttt caggagcagg gtctctaaat ccatttgcat acggaaaatg
      781 ttctgtaaat gtcatttctg ttattccaac atccattgct ctttgtacat atttttcaaa
      841 atcatcctta cttccatgag gacaataagg tgaatgtata tgaccatctt tcataaataa
      901 ttcactcctt ttttaaagaa aaaatctaat ataaatgcat aaatctttta acttcaaaat
      961 aatattttta atcatgctaa tttttattat atctaatatt agagtaattt taaactgcct
     1021 tttaagtatc ttatcattag tactattttc aacttaacat taatattatt ttttaactta
     1081 acattaatac tatctttaaa tagtaatact caatatataa ttacttaaca taaaataact
     1141 caaattgctt gatgaggctt ttgcagtatt taaagaatat ataattattt aacattaaat
     1201 aatccaatct tgtaataaaa tttactcatt aaagtatatt acagaaaata cctcaaaata
     1261 aactttttag ttcatgatat actctttttt gcatgaaatt agatatattt ttatcatttc
     1321 aacaatgaaa gggaggctta tatccatctt tagacaaccc cttagataaa atttttaata
     1381 attaaacatc acaaaatata aaaatcgctt atatcaaacg tataagcgat taattatatt
     1441 tacatatgtt ttttagcctc taacaacatt ttattagctt cttccatata ctctatagcc
     1501 ttttcaatac tctttgctgt ttcttctttt ccaatagctc tagccttttc aacccactct
     1561 ctaaatcctt cttcatgagt ttcattgtga tttatccaat gtactaaaag tatttttaaa
     1621 gttttttcat ccttagactc tgatgattcc tcatgactat gttcgtggtc gtggtcgtga
     1681 gcatggtcat gtggatgttc atgacatata tctccatgag tatgttcgtg gcagtggtca
     1741 tggtcatgat gtccatgatg atatcttaca atctctttat caaaaatcat tagttattcc
     1801 ccctgttatt ttatattaaa ctcttttttt ataaggttta cagaatcttt tacaagtagt
     1861 gctaatggtt taccttctaa tccaactaac tccacatttt ctggcataat aggtaaaagt
     1921 atcttaagtg cctcactgtc tgcaatagct tcacttatat tacaagtaac ttctcccatc
     1981 atagaattag gtattattac agaaataggt gcaactatta tatttgcttt tttagctgat
     2041 actactatag cattttcccc tgtagctcct ttattagcat gagctttcat catagaacta
     2101 gttgctatag aattagtccc tagtgcataa atttctacat atgtaggtaa ctcttctctc
     2161 aaagaagaaa ctatctgggc tccaatacct ccacccattc catctatcac tgctattatc
     2221 atatgaaatt cccctattaa ttaattttct tgtattatta tcttatggtc catcaatctt
     2281 atttctttaa ttatcccatc tattatcttt tgctctccta ataaatccgt taaataaatc
     2341 tttccatcac aagggtctat attaaccaca ttttccataa ccttttctaa ctcattatta
     2401 cttttacaaa tgaaagctga tgattcacac atataaatct cctcctaata tgatttttat
     2461 taaaaatcaa aaaaagattc ccttaggtat ttcaatacac caaagaaacc ttcatgattt
     2521 cagtttttgt atattattat accttaagta tatcacttta atttatgtaa agtcaattaa
     2581 agttaacgct ttttattata ctcttcattc ttgtattttt catatagttc tttaactaat
     2641 tgcttctcat aatcagtcaa atcgccctcc acaaactcaa cattaaattg ttcagctaat
     2701 ccctttacta tgtttttctg tagaatatct atgtctattt ttctgccaat ttcattttct
     2761 attccacttg cttttttagc tgtaaatttc atagctcttt ctttcaattc tggtgtctta
     2821 gtttttatta atttaaacaa tttttctaca tcaaaatcca gtattattga accatgttgt
     2881 aataaaacac catcttttct gctttgagca cttccaatta ccttcttatt attaatagta
     2941 acttcatatg atgcatgtgc attaaaacat gcagctgata aattttccct acttattctc
     3001 tcgcctctat ttaaattatc tgtttctata ccacttagat tcaacccttt aactagacct
     3061 tcacttatat atctataaga taggttgatg ctcttatcga ttaaaggatt atcttctcct
     3121 ataattatac tataagttag ctcattatca tgtaatacag ctcttccacc tgtaattctt
     3181 ctagtatagt ctatattcat acatctacac ttatctaaat ctatctcatc ttccaatttc
     3241 tgaaaatatc ctatactcaa acaagctggt tcccatgtat aaaatctaag agttggctta
     3301 ttatgtccct tcttatatgc agtaaatata gcttcgtcaa ttgccatatt cattgctcct
     3361 tcatatgatt tattatgtat aactctccac tgattcatga ttattacgcc cccattttta
     3421 gtataaatat atcctagaaa tcctctatag atttatttct cttattgcta aatctatctc
     3481 actttgattt aaaggtcttg aatagttgta tatagtactt cctggtcttt cattatagta
     3541 tggtctatta caatccttac aacctcttat ttcaaaagga tagcctttgt ttatgtcttt
     3601 taaaatatca ttgtcaagct ttatactatt taagtatcca tttttaaact taaaatactc
     3661 ttttggataa cctttattta tcatataaga cattaactgt acccttctat aactttctat
     3721 gcttggttga tttatctttt ccattttagt tcctctaact ggagtaaatg caaataaact
     3781 aatcgttaca tcattttcct ttaaataagt atataaattg tatatatctt catgactttc
     3841 acccatacca actattatat gtgtacttat tttattggga tatgattttg acatttcagt
     3901 tataaatttt aacttttcat catagttatt tccttttatt ttttcatata gttctttatt
     3961 tgctgcatct attgctattc ctattttatc aactcctact gaaaaaaact tctttatctg
     4021 ttcatcattt tctaattttg cagatagtga aattggcatg tctatcttac cacttatata
     4081 gtttataaag tctaaaacag agttatgtgc ttcttcactt gccattgatt gaatacatat
     4141 tctctttata ttttttcctt tataagcttt taaagcatct aatatttctt cttttgaaaa
     4201 ctctggccaa attactcttg ataatttatc ttttctagta ctactttcta ttgattgaga
     4261 acaaaaactg catttattta tgcatttttc tccaatcatt atatatgctg tggtaggagg
     4321 tatatcgctt tttttgttta atatcccaag ttctattgct gtcccaactg atagtctaat
     4381 cataaaacgc aacactcctt acactcattt attgtcatat tcatttctat agccttattc
     4441 tttgcagacc ttgatggtaa tactattcta ttgactccac acattataga taattggtct
     4501 agctcttttc tatacactcc tcttggtctc atacaaccta gattaatttc agtatctggt
     4561 aaattaattc tagcttcaca taaaatatct gctacccctt ctaactcagg aggttctaca
     4621 ttttcatact cagtcccttt tgttggaata aggactataa atgtaagctt attaggagga
     4681 tttttttgta ggtattctat aatttcatat tcacccttta tttgtcctcc ttttaaacca
     4741 atacatatat gtggtgctac ttctgtatgt tcttgtattg tattatagac ttcaatataa
     4801 tcttctttac tctttttcat cttataaact tctcttattg tctcatcatc aaacactaaa
     4861 tcaaaagaaa ctatatctaa atacttacac aactctacaa tgctatcttt atccataaga
     4921 cctaaatgag cattcaatct atatccttgc tcttttaagt ttttaatggt attaatatga
     4981 gtatttatag ggacatctcc ctcataacta caaccgccac tcaatagaaa gctagttata
     5041 ttccttgatt gtactttttc ctcatattct tctattggca ccatattgtt taagtagtga
     5101 ccgttgcaat gggcacattt caaactacat tgattgcttg tagtactaag agctagtgtc
     5161 tggttaggat aagcaaactc tattttattt ccaaaattct ttaatttaat atcaaatgct
     5221 atttccattt tttcagatag attcttattc atagtttatt ccttcttaag tacattttac
     5281 tcttctagta atttctttgc tgttgggaac tgctctatat tcgtatgtgg taagaaacat
     5341 gcagaaataa attcatccat ataacttgga tatacactaa gttcaacata agttatttga
     5401 ttacctattt cttcaagttt attttttgca tctttactta taagagctaa gtaagaacca
     5461 acaagtgaac tattccctat ataagtaaat ttttctctat caatatcagg aagtaaccct
     5521 ataattattg agttttctat gtctaggttg tttcctatac cacctgctat ataaacccta
     5581 tctaatatac taaaatccat acctaaacta tctacaagag tgtatgcgcc tgaatatata
     5641 gctcctttag ctctgatgaa attatctata tcaacttcat tgactactat atcattttct
     5701 aaatcaaatt cttctttaaa tgctaataca tactctccta tctcatgctc attaaatcta
     5761 actctcttat tatctaaatc cctgtatatt ttacctcttc tatctattac ccctttagta
     5821 atcatttgac agattaaatc aattatacca gaaccacata taccaactgg tgcacattca
     5881 tcaataatct ttaatgttgg ctctaaagtt tctttatcaa taattacttt ctctattgct
     5941 cctgctgatg ctctcattcc acagcttatc cctccacctt caaaagcagg tcctgctgaa
     6001 catgcacagc tcatcatata atctttattt ccaaatacaa tctctccatt agttcctaaa
     6061 tctataaata aaacattttc ttcacttgac catatccctg ctgataatac acctgctgtt
     6121 atatctcctc ctacataact tgcaacagat ggagctaagt acacatatgc actgtcattt
     6181 acaaaaagac caacattttc acccataagt tttggagatt ttaaaaatgg tggtatgtaa
     6241 ggttcttgtc ttaagaagtc tgtatacaca cccaagaata aacttgtcat agttgtattc
     6301 ccagcagcca ctaatgtaac tacatcttcc ttatctattc catttctttc atatattgtt
     6361 tttaatagtg gatttatagt ttcctcaact atagctttat gaagtgtctc taatccattt
     6421 ttcttagttg aatatataat tctatttata acatctgctc catattttat ctgtgcattt
     6481 ccagaagatg ctttatctac tacctctttt gagtataggt ctactaaaca aacaactact
     6541 gaagttgtac caatatctat tgcaactccg tataaggaat tttctttatt tccttgttct
     6601 atatttatga ttgtaagttt cttttgtttt tgaacatatg ttattgtaac cttaaaatca
     6661 ctctttctaa atacagttgg catttttcta agtatatcca atctaaaatc tatttcatta
     6721 tatcctaggt tatttctaac atatctttcc aatctatcta catcacttat attatcatct
     6781 aaatttggtt cttccatttc tatgtatttc ttttttatat ttgttttaaa ttgaaggtta
     6841 tgttcttcaa ttattttttt agctctttca aaaatttccc tatcctcttt tttattacta
     6901 ccttctatct tcataccatg catagaagat gatactttag aaggaacttc aatttctata
     6961 tctgatatta cctttgtaca acaagctagt atataacctt gctcccactc atcatctgtt
     7021 atatgtcttg ttttttctgt atcaactttt ccattcaata attttacttt acacttccca
     7081 caagacacgt ttccattaca aggagcatct atgaatatat ctgcatttct tgcaacctct
     7141 agtaatatat ccccctcatt acaataaacc tctttattgt ttggtgtaaa acttactttt
     7201 atcatgttat ccctcccaat ttgttctata tatgataaaa ggaaagtata gctaaatttg
     7261 cgtgcactca aattttacta tactttcccc aattataata atatcttaat ctttaattat
     7321 tctaatccag cctcgtattc tttatcactt aataacgtat caagttcagc cacatcagct
     7381 agttttactt ttaatatcca gttttcatat gggtcttcat ttatacattc tggctcatct
     7441 tctaattttt cattaacttc caatacctca ccattaacag gagatattaa atcagaagct
     7501 actttagaag actcaactac tccaaaatct actcccttag ttacagtatc ttctacttct
     7561 ggtgtttcaa caaacaatat ttctcctaat tgatcttgag catagtctgt tatacctata
     7621 tatacaacat ctccatcaat tacttttacc cactcatgat ctttagagta ttttaattct
     7681 ggtaataatt tcatttcgat agacccccaa tattatatta ttgattaaat tacttaaatc
     7741 atattaatat tatatcatat tattttaaat tttccatcaa ttacattaca ctttccatag
     7801 ctaatgctgg atgacctttt tcttctaaga aagctaatac tccttcagaa tcgtcagcta
     7861 tagtttcatc acaaaccata tcacagaagt tttctattcc agtcatttct ttaactgtag
     7921 catttaattt atcagctaca taatctttta attcttttgg catccaaact attctttctg
     7981 gtccaccttc tgcataagcg aattttttag aagatatgaa atgtcttccg tgtcccataa
     8041 atcctggagt ttgaactcct ccacctgtca tagaagcaag ttctccgaaa gtcataccaa
     8101 ctggagtaac acttgcaaat tctctgttaa ctacaacaaa tccatttgct tctggcatta
     8161 taccacagat acattcgaag catccacaag aagtcattgg gtcttctagt atagagtata
     8221 atgtaacaga ttcaactgca ccttgagata tttggtttac agtttcattt actgaattcc
     8281 atacacctgt tctatcatct aagcactcgc ctttttctat tggttgacaa ggacctgttg
     8341 ggtctaattc tttagtagcc ttagcatcta accagctaac tgctccacaa agaccaagtc
     8401 tttcaggagt aacaacacaa acgtgagctg gtgcaaatga ttgacataag ttacaagaat
     8461 agaaagtatc aacactttca tcaactaatg aagctaatct ttcatctcta gcattgtact
     8521 tagctattgc ttctcctttt aattcagaaa ctttttcagc atctgtaatt atagttattt
     8581 cacacttatc aactactgat tcaaattcat ctaacatctt agcatataaa acttctccta
     8641 tatgctcaag tctaaatcct ttatcaaatg catctttaga gattcttacc caagccatat
     8701 ctctttgtcc aacgtgcatt acaccttcta tatagtttaa gaagtagtgg aaacgtcttt
     8761 caagaactgg ctcgaaatct tcttgcatat ttttaccagc tattttaact attacagcaa
     8821 gtggtaatct taatacacca tctgcatcaa cttcatctat atttggtcct attatttcta
     8881 tcttatggtc ttcaacttct gttgcttctt tcttaacaac aagctcccaa gcttcagttc
     8941 tgtttccacc aaactcagca aacatatcgc tctttctaat tctctcacct tcaaatgctg
     9001 ctgcaaatga aactgggata ggtatttcag taacttttat ttttattcct ctagcttcta
     9061 atgaagtagc tactatctta tcgtaatctt tttgagttaa taggttcatt ggaacttcaa
     9121 gaactgtttg gtcagttata actgggaatc ctaaagctat tgctccagca cctgcagata
     9181 caactaattc acttaatggt ccaaatgcat taacaaatgc aggaactcta ttagctgtgt
     9241 actctaataa tccgtttaaa tcaccaggag ttaatccacc gaatattaat gctgctctta
     9301 ctgcaacaga tacaacgtgt ataacagaag ttacatcata tcctagaggt ataactctta
     9361 gctcaagacc catttttact ccagcttcta tagcttggtc tataacttta ccaactaaga
     9421 aagttagtaa acctttagat tggtaatctt ttataacttt tgctgctgat tctgcatctg
     9481 gacactcacc aagaacaact gcaacaccag gtatatctcc tgtaactaat ggtacaccaa
     9541 gtgatctgat tatagggtca gttatatgac ctgcacatgg ctcactatat ggagcatcca
     9601 tagttgaata ttttaatgct tcaattactt ctgctgctaa agctgtagct aaaccagcat
     9661 taaaagcatg ttctagtaaa tgagttctat ttattaaaga ttttactact tctaaagcac
     9721 cttctaagtc ccctaaagtg ttcatctttt gtcctgttgc agcatatata caaggtaatg
     9781 aatatgctgt atcagggaaa gcaactttat gctcttttcc attcttttct atagcttcag
     9841 ctaacatagc ttgagccgca cctaaagctt gttctgaccc tgtaaagatt atattatata
     9901 gattcattct atttccccct ttaatattta cacctatttt ttattaacta taccctaaaa
     9961 accctagaaa gcaaatccac cttcatttaa ctctaagaaa gagtctgcat ataaagatgc
    10021 cccgttgatt atgtcacaag attttacagt tttcattaat tctgggtcac atggattcat
    10081 tatagctgaa ctaaatccat tagccattgc cattgctaag aatgcactat ctaaaacagg
    10141 tctaacatgc ttaggacatc cattagatac gttagataat cctcctgtag ttaaaagtcc
    10201 catctcagtc atcattttta tagcttctaa aacttctact tgtttttctt gcataccttt
    10261 tataactaag cataatgggt caaatagtat atcagttggc tccattccta acattaagcc
    10321 tttttctaat atttcttggc agtaagccat acgctcatca ttatctcttg ggataccttc
    10381 ttttgcacat aaacctataa ccattgcttc gtattcagct gctatatcta ttaaatcaaa
    10441 tcttgaacca gcatcagcag agtttattat tggttttgca ttagttctat cataaacttt
    10501 aagtccagct tcgatagctt ttttattagc tgtatctaat gctataggaa cattgttgaa
    10561 ctcagattga agtaatttga ttccccatgt cattatttct tcgccatctc tttcggcagg
    10621 tcctatatta aaatctatat agtgtgctcc tgcttctaat tgctcttttg ctctttttaa
    10681 gattggagct gggtctcttt ctgctaatgc ttttcttatt gaaggtgata tacaatgtat
    10741 tctctcacct ataatcataa atttttccat ttataatgtc cccctttaaa tttaatatta
    10801 atagttagtt aatattttat tagtttgttt ggcatgcttt ttcttggtat ccttttaaga
    10861 acttaggtaa ttccatagat tcctctggtc ctataactac attccatcct ggtaagttat
    10921 cttctatatc acctttaagt actgcaactt ttcctggaat gataaggtct ttacaattag
    10981 ttacttcttc taccttactt tctttaatga aagcagagat tgagtttcca ccaaatttac
    11041 cagcagccca agatgtaaga actgaatatc cacctgcatc tggtattact aaccatactg
    11101 gaacttttga tctttctata tcaccagcaa ctatgaagta agttaatgca aagtcaacag
    11161 taactaatac tggagaattt tcatctgggt tgttgattgg ataaatcttt ggttcaactc
    11221 tcattggtct ttgtgggtca gtgtatatgt tttgtcttag tgcaaataat ggtaatgcct
    11281 tagcataact aatatcatct attactatta tagaaccgta ttttatagtg aatattgatg
    11341 ataaagcaac ttccatcata gggtttgaat tagataatct atttgcaaat actattgaag
    11401 gatatccaaa tgttctatct tgctctttta aagctgttct tctaacttgt atagcattag
    11461 tgtaagtatc ttttatgttt tctcctgtta catctaatac taattcttta tatccagcag
    11521 cttgaattaa ttcaactgtt tcatataatt cttctaagct accagctttt acacctaatg
    11581 gtaaacttgc tcctttaact acttctatca tatctttgta gttctcttta gttgctccat
    11641 ataccattgg tctagcatct tttaatactt caacagcttc tttaactact tgtgcatcat
    11701 cacaagctaa aatataagct acttctaatc cactgccttt tattttatct attaatttta
    11761 aatacgcatc tttatctcca caatactcta aaacagccat ttcaactttc atttgttcac
    11821 caattcttac atagtcaact tttttcatgt tagctatttt agcatctact gcttcatcac
    11881 tcatacaagt acagaatgaa actgcatatc tatttctact tactaatgtt ttttcatgtc
    11941 taaataatac tgtttctcca cctagttcat actcagatgc accagcacca acttttaaag
    12001 ctttcattaa aggtgcagta gcctctgata attttgctat agcgtcatca gacatatgtg
    12061 ggcatttacc tacttctaca gctcctgaag caaccttcat agagaaagcc atacaagttg
    12121 ggaatccaca atccttacag tttttctttg gtgttaattt aaatatatct aaagctttta
    12181 atgccattat tttttccccc taaccttaac aatttaatta agctaatgcg ttaaccaatt
    12241 cttttatagt ttctattgat tttggatgac gaagtataac tgcgtttgaa ccacctacta
    12301 aaacacttgc tgcagttgaa acttccattg ctatagttct ttcttctgga caaccccaat
    12361 ctggctcatc ttctattgga gctatagctt ctttaacatg acctacttca aaagctactg
    12421 gtgttatgat aggcatttgt aatgtcttat cattttgacc aaatgctgca agtctaattc
    12481 tatccatagt agatgcaacg tactcatatc cataaccaac tgctgaacat ccaacattca
    12541 taactatatt ttcaccttta acgcctagtt gagttaacaa tacgtttaat tgtttagcta
    12601 ggtttatatc aacagaagat tcagctccta ctttatgtgc ataagccata ccagctgatg
    12661 ctcctactcc tttgtagtta tcttctactg ctgacataaa taagcagtta tgtccatcca
    12721 atgtttgagc taatttttca aataatttac catctttttc atgatttcct gaaccagcta
    12781 caactaaagg taattttatt gcttcaatta ctgccttagc tacatcagca cattcatcaa
    12841 ctgacttatc taatccattt gggtcagaac catcaaattt taaacaaata aaatctgctt
    12901 gtgtattagc ttcaacatat ttagcccact ctactggaca attagctaca tctttgtata
    12961 attccttata tgaatcagtc cagctttcag gataaacatc tgatatttgt atccctattt
    13021 ttggagaatt acctacttct ccatcaaaac tataaaaagg taatacattt tccccaccta
    13081 atttaattgc tttttcccct attcctactt caacttctga tatttttcca gaatattttt
    13141 gagtagacat tttaaatgcc atattttttt cccccttaag cctattttaa ttctaattta
    13201 gcaaaaattt ctttcatagc cactttagat tttgaatctt caggtaaatt aactaaagga
    13261 atacctgtag aatcatattc atatatcaat tcatccatag gtacaacacc gattagctct
    13321 aggttatgct tttcaatttc ttctttaaca ccatcattca taaccccatt tggaacttta
    13381 tttacaatca aaagtattct tccaacactt aactttagtt cctcagctaa atctcttatt
    13441 ctagcaacag cttgtatgct acgtcttgag caatcactaa ccaataaaag tgtatcaaca
    13501 tgtctagtga cttttctact tagatgttcc ataccagctt cgttgtccat gactaagtat
    13561 ttgtaatgac cagatatttt gtttacttgt tctctaagta tcccatttac aaaacagtaa
    13621 catccttcac cttctgacct acccattact aataagtcat atccttcgcc ttcttcgatt
    13681 atagaattta atctaaattg aagatattgt gcctttgtca taccaccagg gaatgcattg
    13741 cctaactttt ctctttgatt tacttcttct cttatttctc ctattgttgc ttcaacttcg
    13801 atacctaata cttcatttat gttagcattg gcatcagcat ctacaactaa tactggtccc
    13861 tttttgtcct taaccaaata atcaatcaaa agccctgtga gacttgtttt accagttcca
    13921 ccttttcctg caacagctat attgtatccc attttaagac ttccccctta aatctagcca
    13981 cagagcctta tgtactctgt ctgtctatta taatttaggt acagaatgaa cacattctcc
    14041 atgtacatca tgaagtgctt ccattagccc ttcagataaa cttggatgtg gatgtattgc
    14101 atcccctact tgttctactg ttaatcctag atgaacagca agtgacaact ctgtcaataa
    14161 atctgtagca tgaggtccta ctactgctgc acctattatc ttatcagttt ctttgtcagc
    14221 tataactttt acaaatcctt ggaaatgtcc tatagcttgt gcttttccaa gacctctaaa
    14281 atcaaattga cctacattgt attctacacc ttcagcttca agttgctttt cagtcttacc
    14341 aacaccagca acttcaggtt cagtataaac acatcttgga attgctctat aatctactac
    14401 cttagtctta cctaaagcat tttcaactgc tacgatacct tctttagaag caacatgtgc
    14461 taggaaagga gtatctatta tatcgcctat tgcgtatata ccttctacat tagtttcaag
    14521 atgttcatta actactactt ttcctctttc catttcaatt cctatatctt ctactccaga
    14581 attgtcaagg ttaggtcttc taccaacaca tactaatgca tattgtgctt ctataacctt
    14641 tccattagaa agagttgcaa cagccttacc atcaactact tcacaagttt gtactccaat
    14701 acctgtaata accttaattt tatctttctt aaattgtcta agtagttgtt tagctacatc
    14761 tttgtcttca tttaatagta tttggtcaac catctcaact atagttactt ctgttcccaa
    14821 ggctctaaag aactgcccta tctcacatcc tataactcca ccacctacta ttaacataga
    14881 ctcaggtatc tcttcaagtc caagaacttc atcactagtt attacaactt ttccatcata
    14941 tgggaacatt cttggtacaa ctggaacaga accatttgct agtattatct tatctgcttt
    15001 tatagtttct actgttccat catctttagt tacttctata gtgtttttat caattaattt
    15061 tccaaaacca ttaactaagt ttactcctct tttttcaaat aagaactcta taccacttat
    15121 taattggttt acaactttat tttttctttc cattatggca gtgaagtttg gcttaactgt
    15181 accatctatt tctataccaa aatcctttgc ttcttttaca gtattcaata ctcctgaaga
    15241 agcaagaagt gctttagttg gtatacatcc tgcatttaag caagttcctc caactcttct
    15301 tttttcaaca actgttacat ctgcaccaag catagaagct tttatagctg ctacatatcc
    15361 tcctggtcca ccacctacta ctactatctt catctttaat tcccccaaac ctaatataat
    15421 ctacaatctt gcaatactat aaacctgcgg catccagtat agcaggtata ttctcttctg
    15481 ccccaattgc cattatatga acaccatcac ataaatcttc ttcttttaat tgtttgataa
    15541 gttcccctgc catttttatt ccttcactta cccagttttc ttttccagct gctcttaatc
    15601 tttcgatttg ttcatctgga acaaatattc ctggtacatt tgcagtcata aatttagcca
    15661 ttcctggtga ttttaaaggt actattcctg ctaatacctt gcaatccata tcccttgtta
    15721 attctctaaa ctttctcatt gtatttatgt cataaagtgc ttgtgtttgg aaaaatttag
    15781 cccctgcttt tattttcttt ttcattttta ataattgaac ttctattgga gaatattctg
    15841 gagttactga tgctcccaga taaaaatctg gagaaccttt taacttatta ccagccatgt
    15901 ccgtacctgc cattaaagtc tcagcagttt gaagtatccc cacagaatct aaatcaaaca
    15961 ctcccttagc ttgtggatgg tctcctacgc tagtatggtc tcctgttagt gccaacagat
    16021 tgtttatccc aaatacacct gctgacaaca tttctccttg tatagctatt ctatttctat
    16081 ctctccctgt tatttgaata actggctcta atccagcatc ttttaataat ttacatgtag
    16141 caagagaagt tgctctcata actgccgatt gaaagtctgt tacatttgct gcatgaactc
    16201 ttccaactaa cggctttgca cattctatta aatgtgaaag gtcagtccct tttggaggag
    16261 ccatttcagt agtaactgca aattttccac tttctaatgt ttctcttaat aagctcatct
    16321 ctaatccccc ttttaacctt ctaaaaaaaa cttttatgaa tttgctgctg cttctttctt
    16381 tttctttgca cttaaacttc ttggattatt ttgcttagaa tagtcttttg gtggtcttat
    16441 ttctgctaag ttatctaatt gtcctatagc ttctagtctt tcatatatct ttatccaagc
    16501 acaatcattc tctgagttta cttcacactt accattttta gctccaccac aagccccatt
    16561 cataagacct ttagcacaca tagttactgg acatatccca cctgtccaac caagttcaca
    16621 gtctccacaa gccttacagg cttcttcata ttctccaact ctttgtactt cccctataaa
    16681 caaagtattg ttggctggat aaactggctt atcttttaaa ttcttaacta ttgtctgtgt
    16741 accatctcca catgctaaag ataaaactgc atcagcttcc ttagtctctt cctttagagc
    16801 ctttaaatcc tttttagact ttaaaaggtt acaagcaggg tcaagcatta catatcctaa
    16861 gatttttttg ccttcacctt caagagtttc cttcattttt aaaacttctt cttccccacc
    16921 acttttacaa gttgctgcac attgattaca accaactaaa accagcttgt caaaatcttt
    16981 taagtaccct agcacttctt ctaatggttt attttcagaa attatcatat ctacttcccc
    17041 cctaaacctt ccacatatat atatctattt attgttttgt aatttacaag ccttaactac
    17101 atgttttgca agtatagatg ttgtaactga accaaccccc gctggaactg gagttatcat
    17161 tgatactttg tctaaaactg catttgtatc aacatctcca cataaatttc cttcttcatc
    17221 aacatttata ccaacgtcta taactaccgc tccatcttta acaaaacttt catcaaccat
    17281 cttagctcta ccaatagctg ctattagcac gtctgcctct gctgctacac ctgatagatt
    17341 tttagttttt gaatgacata ttgtaacagt tgcatgttca cttaataaaa gcattgatac
    17401 tggctttcca actaccattg atcttccaag aactgttacc ttacttcctt ttaaatcaac
    17461 attatagtgt tttaatatct caactactgc tgtaggtgta catggtggaa atccactttt
    17521 atccccttcc attaccttag cagagtttat tggactaaaa caatctacgt ctttttccgg
    17581 tgcaataaca tattttataa cttcttcatt taagtgtttt ggaagaggtc taaaacataa
    17641 tataccatta acatttttgt catcattaac tctctttaat acatcaatat attcctcttg
    17701 tgttatatcc tcagctaact ctaacacttc tgttgttata cctatgtttt gacatctctt
    17761 tagtgctcct ctttcataag ataaatcatc acttcttgcc ccaactctaa ctattgttag
    17821 tttaggattt ataccttctt taattagtaa atcgacttct tttattaatt cttcactaat
    17881 cttatcagct acaggttttc cttttataat ttgtccttta gttgacattc cttccataca
    17941 ttcacaactc ccctacaagc aaacttaaat taatttattc tatataccac atattaataa
    18001 catagtatct actttcgtca tttaaaaatt attttccaag agctttttca actttggcat
    18061 aaacttcatc acaaattttt acaccatctt caactaactt atctacttct gtttttacct
    18121 tattaacata ttctttatct tgtatagaat ttatatttat aactacattt aattgtccac
    18181 caagtatagc agctcttaaa cactgaactc ctacaccaac atcacttata gcaagtcttg
    18241 aacctttatc aactaaatct tcatgtaact taatagcttc atagcaaact cttactatgt
    18301 ttaatggtac ttcacaagca acttttaatg ccttttccat tgtttctgct tttattcttt
    18361 tttcttcttc tgtttcttta ggaagcccgt aagcttttga taatggtaag aaacattctg
    18421 catcatcatc tatcattttt aataaatctt tttctaactt tcctgcctta cttaatatct
    18481 cttttacact ttcttcatac tcagcatact tcttcttccc tattgtaagg ttacatacca
    18541 tactacctaa agccatacct attgcaccta ctagtgcagc agctccccca ccacctggta
    18601 ctgcttcttt ggaagatagt acttctacaa aatcaacaca agttttatct gctattttca
    18661 tgataatcct ccttgaaagg atgtaggaaa atatctcaaa ttgttcgaga tattttacga
    18721 actattttaa gatatctcct actctttatt tataaattaa tttccttaga ataatccagc
    18781 tattactcca ttttcatcaa catctatttt ttctgctgct ggaacttttg gtaatcctgg
    18841 catcttcatt atttctccag taagtgcaac tataaatcca gctcctgctg aaattgttgc
    18901 ttgtcttaca gttattctaa atcctgttgg tctacctaat ttagtttggt catcagttaa
    18961 agaatattga gttttagcca tacatactgg aactttagta aatccaagtt tttctaagtt
    19021 agctatttcc ttatcagctt caggagtata atctacacca tctgctccat aaatcttagt
    19081 agctatagca tttatcttgt cttttataga taaatcatct tcatagcaga atctaaagtt
    19141 attttcttct tcatccaata atcttaaaac ttctttagct actgcaattc caccttctcc
    19201 accttttgcc catacttcag aaagagcaac attaacacct aattccttac acttgtcttc
    19261 aacaagttta agttcagctt cagtatcaag tgggaatcta ttgattgcaa caactgctgg
    19321 taatccatat acttgagtta tgttttcaac atgttttaat aagtttggaa gtcctttttc
    19381 aagagcttct aggttttcgt tatttaagtc tgctttagca actccaccat tgtattttaa
    19441 tgctcttaca gtagcaacta ttataacagc atctggtttt aagtttgcca ttctacattt
    19501 tatatctaag aatttttctg ctccaaggtc agcaccgaaa ccagcctcag ttattacata
    19561 atctgcaaag tgcatagcca ttcttgtagc tataacagag ttacatccat gagctatatt
    19621 tgcgaatggt ccaccgtgta caaatgatgg tgttccttca agagtttgaa ctaggtttgg
    19681 ttttaaagca tcttttaata atgcagccat agcaccatta gcttttaatt gtctagctgt
    19741 tacaggttca ccttcgtagc tgtatccaac tattatgttt cctaatcttt cttttaaatc
    19801 agatatatca cttgccaaac agaacgctgc cattatttca gaagcaacag ttatatcgaa
    19861 tccatcttgt ctaactgctc catcaccttt tttacccata ccatcaacga tatttctaag
    19921 ttgtctatcg ttcatgtcta cacatcttct ccaagttatt ttcttagggt ctattctaag
    19981 tgcatttcct tggtgaatat ggttatcaag catagcagct aaaagattgt tagcagctcc
    20041 tatagcgtga aaatctcctg tgaagtgtaa gtttatatct tccataggaa caacttgtgc
    20101 atatcctcca ccagctgctc cacctttaac accaaatact ggtcctagag atggttctct
    20161 taaagcaact aatacatttt tgtctagttt agcaaatgca tcagcaacac ctatagtagt
    20221 agtagttttt ccttcacctg ctggagttgg gtttatagct gtagttagta ttaacttagc
    20281 ttttttacca gtctctcttt ttagtagatt gtagtcaact tttgctttgt atttaccata
    20341 aagttctaca tcatcttcac ctagtcctaa tttttttgca acctctctaa tatcttgagg
    20401 cttagcttct tgagctattt caatgtcaga tttaaatccc ataactgaat ctcctcccac
    20461 aacctaaata gatttaaaat ataatttttg taaaaattgt attaatatgt aaaaattata
    20521 ttttataaga taattataca tgtaaaatta cttaaaaact agctactaag ctattaaaat
    20581 attcaaactt aatagctagt tatttatcta attacttact tattacctat ctattgccta
    20641 tgtctaacct ttaaccaact taaaatccca aatcaaaatt tctatctatt ttacttatcc
    20701 ttaagtgaca ttaatttatc ttttattgct ttaatctgtt ctttcgttgt ttcactagaa
    20761 tcatatggag attgattagc tctatcagaa tcaataacat cttggttata ataaataaat
    20821 cctaaagatt ctccatcttc taagttttgg attataaatt cttcatcttc cttatttcta
    20881 attttatttc ctactacaaa tactttattt acacctatat catgtccaag tttcttaact
    20941 tttctataag tctgtagact tctctctcct ggttctacaa ctactataaa agcatctaca
    21001 ccttgggctg ttcctcttcc taagtgttca attcccgctt ccatatccat aaccactaca
    21061 tctttatttt gtagtattaa atgtgagcag agtctcttca aaagaacatg ctctggacat
    21121 acacatccag taccaccaga atctacggtt cccaatgtaa gtaatcttac tccattgtat
    21181 tctttacaga aattttcagg gatatcgtct actttaggat tcatcttaaa catttttcca
    21241 aatgaaccta cagaagcagc tgttctatct gaaactaatt ttttcatttc tgatataggt
    21301 actattgatt catatacttc ttttggaaaa cctagtgcta aagctaaatt agcatcaggg
    21361 tcggcatcaa cagcaacaac tctgtatccg tcttcagcaa acatcctaga gagcattgaa
    21421 gaaaaagtcg ttttacctac tccaccttta cctgttattg ctatcttcat aatccctacc
    21481 accttttatt actatttgct ttttagtagt taattttaca gttaaatatt ttttatactc
    21541 tatattttta tacactaaaa ataaactaca gctagtcctt acaggaacca attaaattta
    21601 ccctactagc ttttcaaagc agcatgtata gttttattat tctttaatat tgtttatatt
    21661 ttatttattc agtatattca ttatattttg atgttcttta ctttaatatt tgactttatt
    21721 caactttatt caaccagaaa cttcttataa aaacgtttat tctataactt cactattttt
    21781 taagcttcag ccttttctgc cattttttct tcaactaatg cttcaaagtg aacacgtttt
    21841 ttctctatat cttctattat tgtagctgct aactcttttg gatttgtatt tacagtaaat
    21901 gtagctccaa ctttatcttt taattcacta gttaatatat ccactgctct tgaagaacca
    21961 gctattggag gcattattcc taaataagtg tctataccag aagctacaac atagcatcct
    22021 atagcaactg ctttttctga catccactct ggagctatac ctacaactgg aaggtcacac
    22081 atatccatat ctaagtaatt agctgctgct ccaactaaat ctagtatacg gcttatatca
    22141 acacaagaac ccatatgtag tactggtggg attcctacta atttacagac agttgcaaga
    22201 cctttacctg catatttttc agccgcatca gattccatta aaccaaactt agctgctgct
    22261 gaagctccac aacctgtagt aactactatt atgtcgtttt tgattaattc tttcattatt
    22321 gttacgtgag cctcatttga aactccttta gcattgttac atccaactac accagctgct
    22381 cctcttaata ctccagcttt taaacagtct gttaatggct taactgttcc tgctgggtct
    22441 atatgagagt ttacaactct gtctaattga tttactactg cttctacact atacccaact
    22501 gttgcgcctg attttaattc aggtataagt acttttgatt tatctctatt tttaaagttt
    22561 aaaatagctt ctttaactat tgctttagca tcatcaagtg cttgttcttc tctaaattca
    22621 atataagttg aacctgttat cttagctttt ggagaagttg ttacaaattt agtatggtaa
    22681 caatctgcaa ctctagctaa tgcagggaat atacattgaa cgtcaactat tacagcttca
    22741 actgctccag ttattattgc aagttcttgt tgatggaagt ctcctgctat ttttacacca
    22801 tgtctcattg taatctcatt acctgtacaa cacatacctg ctaagtttat tccatcagca
    22861 ccaacttctt tagctaaagc tactaagtct ggctcttctg atgctaaaac tatcatttct
    22921 gataatgctg gctcatgtcc atgtaatatt atgttaactt tattttcttc taatactgct
    22981 aaattcgcct ctgttcttct tggcactgga gttccaaata agatatcgct tagacgagta
    23041 cctatcatag aaccacccca accatcagcc atagaagttc taagactcat gtgtattaaa
    23101 ctatctatat caccagtaca tcctatatga gtagagtgca ttattgttgc aatttctcta
    23161 tctatagctc ttggctctat agcgtattct ttccatattt tttgtcttgg ttctggtgca
    23221 ttttttataa atcttaatgt cccaaaaggt tttccaaatt ccattaatgc aatttctgcc
    23281 acttcatgtg ctacatcata gatatctcta ccttctgtct ctacatccca ttctttagct
    23341 aaagtaatta acttagcttc gtctcttatt ttatagtttc catttggatc agctaatgct
    23401 aatgtatgtg ctatatctct agcatggtct gaatgggcag ctgtcccacc agcaaccatt
    23461 cttgcaaagt ttctacctac tatagtatgc tcatctgctc cacataaccc ccttggagtc
    23521 ttagggctta ttctacatgg tcccattcca catattctac agcaaacacc ttgaagacca
    23581 aaaccacatt gtgccttaaa gccttctttt ctatcataca ttgtttctac gccttcttct
    23641 ctagctttag ctatcatctt ctggctgttt aaatcaattg ttaacatttt ttcatccata
    23701 ttcctatccc ctttttttga tttttaaaat cccccaggtg actgacttca tgtcataaaa
    23761 taataaaata aatgctttct aagttgatta tatactaatt ttcagtgctt tgctttagat
    23821 ttaaatattt ttttaacaaa attagataat acataaaaat tttattttct acacttaatt
    23881 taagttgatt ttatcgcttg tttttggcta tttgttaata tctaaaccta cttttttgat
    23941 acttcagttt aattaaatta tgttttgatt tttttaaatc aaatttgaaa tttttctagt
    24001 catttttata ttattgcaga ttgtttgtgt atttattatc tttcactact gtttggactt
    24061 attacagatt tttactccct aaacttaatt agtaaaaatt tcttagaata gcattttttg
    24121 gactaatttt tttgaatttt tttatttttc ctactttaat attaattata gcttcaaatt
    24181 gaacatttgt atatattcat ttgaagctat aattattttt tatataaaac ttttttaata
    24241 cagcattaat ttagacattt tgactatttt ttctacttta attcatttat ttcaagcgat
    24301 ttatcctatt tcatattttt agctacaata gccccatatg caatagaata taatttagat
    24361 tcatgtgaat cagctcttga cactaaaact ataggagctt ttgcacccat aattatacct
    24421 gctgatttag agtctgccat atatgtaaag gctttcccta ttccattacc aacctctatt
    24481 gttggaacca atatgatatc tacatctcca gatacttcac ttttaaaccc ttttatctca
    24541 ctagcttctt ttgaaacagc taaatcaaaa gccagaggac cttctacaat tacattttca
    24601 ccaaattctc cttctttaca agcttttgct aacatatctg catctacagt tgcttgcatc
    24661 ttaggattta ctttctcttt agctgccaaa catgcaactt ttacagtttc cattcctaaa
    24721 gcttttgcag cttttataga attttttaat atctttgcct tttgttcata atcaggagct
    24781 atattcatac ctccatctgt agttataagt aatttatggt atttttctaa ctcatatacc
    24841 ataacatgac ttaataaact atcagttcta agaccatagt ctttatttaa cacttctttt
    24901 aatatgacag atgtatctaa taatcctttc attacaaagt cagcttcttt acttgatact
    24961 aatttaactg ctatttctgc acactctttg atatcatcct tatccactat tttgatacca
    25021 cttaaatcaa atcctatttc ttttgatatt tctcttattt tgccttcatt tccaaccaat
    25081 ataggagtaa ttatatttct ttctacagca tcctttattg ccattaaaac ttctttatca
    25141 tgagctgctg ctacagagag tacaattttc tctcctccct taagttgttc taaaatatca
    25201 tctaattttt ttatcaaaag taacccctcc cttatatata tttaactaaa taataccata
    25261 tttgctatac cttttctcaa tttatttttt atgttataaa gaatttatat aactaaaaga
    25321 cagtttaata ttttattctg ggatttcatc aatagctctt aaaagctttg caggaacacc
    25381 acctacaata gtattttctg caacattttt tgttacaact gctccagcag cgataattgc
    25441 attatctcca atagtaactc ctggaactac agttacatta gcaccaatcc atacattttt
    25501 tcctattgtg attggagaag gataagttgt accacgtttc tctggtgcaa taccatggtt
    25561 aagtgtagcc aataccacat tatgcccaat ttgcgtatta tctccaatct caatcccccc
    25621 ctggtcttga aaatggcaac tgctatttat aaaaacattt tttcctattg tgatgttttt
    25681 tccacaatct gtataaaatg gtggaaacat aaaaaatgat tcatctacct tttttccaat
    25741 taactcagaa aataaactac ggacttcttc tggttcatga taagaagaat ttaacttact
    25801 tgtgattttc attgcctcat tactcaactt aaccataaat tcatgtattt ctgatttact
    25861 agaaacaggt tttctttgat tgatatgtgt taaaaattca ttgatattca ttttatacat
    25921 cctttcaata tgtgctttaa tttatttgta cattctaaat aatattttaa taaaaattaa
    25981 tttaatttat ttttattaaa atatcattgc ttcttttagt gtaatataca attatatcta
    26041 tatcaaatat ctatattaaa tacttaacta tacttaaaaa gcatataaat tgatataatt
    26101 atataataaa taagttaata ttgaggaggg ataattatgg aaataagagt attaagatat
    26161 tttttagcaa ttgcaagaga agaaagtata tctggtgcgg caaccttttt acatataaca
    26221 caaccaacac tttctagaca aattatggaa cttgaagaag agttgggagt taaattattg
    26281 gttcgaggaa atagaaaaat caccttaaca aatgaaggta ggcttttaca taaaagagca
    26341 gaggaaatta tatcattagt tgataaggtt gaattagaat ttgacaatcc caatgagatt
    26401 attagtggtg atatatatat aggaagtggt gaaactgaag ctatgcgact tatagctaga
    26461 actgcccatc attttcatca aaaatacccc aatgtaagat atcacctttt tagtgggaat
    26521 gctgatgatg tgactgaaaa attagacaag ggactgcttg attttggtat tttaattgaa
    26581 cctgcaaata tacagaagta tgattatgca caaattcctg ctactgatgt atggggttta
    26641 ctaatgcgta aagatagtgc ccttgcatca caaagcacaa taaatccaaa agatttatgc
    26701 aatatacctc ttattatttc aagacagaca cttgttgata aagcaatttc taaatggctt
    26761 caaggcagta atgaaaaact aaacattatt gcaacataca atttagttta taacgcttct
    26821 cttttggtag aagaaggtct gggctacgct ctgtgtttag atagactcgt caatactaca
    26881 gaaaacagta atctttgttt tagacctcta tctcctcctt tagaagctaa attaaatatt
    26941 atatggaaaa aacatcaaat attttcaaag gcagcagaaa aatttttact tcaaatacaa
    27001 aaagattttt cttctatcat gttggataca tctaagttgg atacacctaa agaaaaataa
    27061 atcataataa aaagtgagag tgtctcaaaa tgaactttgc actgtataag gcactctttt
    27121 ttatacaaag taaaatatat gttcatcatt tcagcactaa aaaagaggct tatctctatt
    27181 ttgagatagc cccacttaac caataaaatt aaatacaact taacaatatc cttacttatt
    27241 tattccaagt tctcttagac aagcaatcgt aatttcatct tgtggattta attcataagc
    27301 tctttctata tagtaagttg catcatcaat atcaccatta tttaaataag ccattcctaa
    27361 attgcaaagt atttctgggt cttcctttat cttagccgct tgttcaaaat actctattgc
    27421 tttatctaaa ttattcttat aagcctcaca aagccccaat tccactatag tatccacctg
    27481 attaggcttt attatcaata tcttctcaag atacatctta gcctgctcta tttcgcccat
    27541 acccttatat ccaagtgcta tcataaacag caagttccac caatccatat gttcttcttc
    27601 taatggaagt aacttctcta atccttcctt aaacttacct tggaatacaa gtgtatatcc
    27661 ttcttcatat tgaactttaa agtccatctt gcccagattc tcttgtacct cagctactaa
    27721 atcagcatct aaccctagcc ttaacgcttc ttcccaagtc agcttagtct ttaaatattg
    27781 accttgatta taataatggt agcctaagtg ataataacct aatgcaaaat tctcatcaac
    27841 atcaattacc ttttcaagct tagccatggc ttcaagtaag aatgcattca ttgccttatc
    27901 atccatatct ttttgatact gtttagctat ctcttggcaa acaatcgcat agttatacat
    27961 tgcatctaaa tcatctggat acattgttat aaatgctttt atatatatta aagaatctgt
    28021 atgttcacca atctcaaagt attttctcga catatatccc atatacgatt tcaaatccaa
    28081 atcaatatct tttgataaag catttaaaaa cttctcatac tctgcattgt ttttaaactt
    28141 actatctatt ccaattatat atatcattgc atctgctata gacattgaat ttaacttatc
    28201 ttgagcagtc ttttctttta tcccttttac taatacctca ttttttatag gtacatctaa
    28261 tccacctttt ggcacttcat agctttttaa tttaaactct ccaccatgtt ttatagttat
    28321 aaaagctaat tcttctgctt tttttagcaa atatttttct atcttaaaat ccattttatc
    28381 ctcctaaata cttaaatctc tactttatga gttttagatt tctcaaatat ttttttatca
    28441 tcatactcaa ataagactac attttctact tctcttattt ctgtatttat atcatttttt
    28501 aaatccacaa cattaaatct aaatggctca aaatgaacat attttattat atgtttagct
    28561 ggtttatcaa caaaactagg taaatatgtt gataagttat cattatcttg taaaaataca
    28621 tggcatctat tcttaaaatc atccacatct agcttattaa aaaactgtgg tatatttgat
    28681 gttttaccca gtgatatata gtcatatttt aatatatcgt tgaatacatc attatctatg
    28741 ttgatttttt cattatagaa atccatcaat attttatata actgattctt tccttgtgcc
    28801 aaatcaaagt agccattttc atcaaaatac ttggcaaaaa cctcaaagaa tttaaaaggg
    28861 ctttttttat aaaatctgcc tataatatat ctcatagata acacaaagtt tttagaattg
    28921 tagtatctct ccagtatatc ttctatatcc tttagcttca aagtttcttc atagcttata
    28981 taattattgt ataaaaactc atatggagca taatccttat atttaaaacc atgctcatct
    29041 gcaacttttc tcatacctgt ccctttaatc atctttaaaa agcctaattg cagatgctct
    29101 atacctagat taaatacatc attaaaagaa ttttcaaagc tcttataatc ttcataaggc
    29161 agtcctgcaa ttaagtctaa gtgttgatgt atatttctat aagatgctac agtttgtaca
    29221 acatgagaca actttttaaa atcatccctt ctacctactg catcaagtgt tttttcattc
    29281 gttgactgaa cccctatttc aaattgaaaa agaccttctt tacaatcagc caaaaactca
    29341 agcatcttat catctatcaa atacgctgta acttcaaaat gataagttgt atatccatta
    29401 tcatttttca taagaaaatc catgatttcc atagccactt ttctatttgc attgaatgtc
    29461 ctatctataa actttatttg tgatactctt gcatctatta aagcttttaa atcactcttt
    29521 atcctgtcta aactaaaata ccttagacct ggaatagtag atgataaaca gtattggcag
    29581 ttaaaagggc aacctcttga tgattcataa tacactattc tattttcata ttcttttgga
    29641 ttcaagtttt cataaggact aggaatctca tccaaatctt gaagtaattc cataggctta
    29701 ttcactatta tctcattgcc ttgtctatat acaagtccat ttacatcatt tatttccatc
    29761 tttccttgca agtgaagtac taaatctcta aaaattaatt ctccctctcc ataaagtata
    29821 taatccacaa aatcatactt tttcattgcc tcataactat cataactcac ttccggtccg
    29881 cccaaagcta ttttagtatt aggtctaatt ttttttagat tatcacaaag tttaactata
    29941 tctcccacat tccagatata tgttgagaat aatattatat catattcatt tttatatata
    30001 tcctttaaaa tatagtccaa atcgttgttt atcgtgtatt ctttcatatt aacttctatc
    30061 aaatctctta caaattcttt tagatatctt atagccaaat ttgtatgaat aaacttggaa
    30121 tttaatgttg ttaataaaat tttcaaatat ttctcacctc tttttaagat tatactgtct
    30181 ttttgtaccc cttataggtt aaaatagtat atgtaaagtc taaataatat ctatataatt
    30241 aatttattaa tttcaagtaa tgtgaggaaa ggaagttttt tatggaatta attgaaaaag
    30301 tatctatcaa ctctttatca aaaagagatt tactacttat tataaagtct cttgaataca
    30361 ctaatgaaaa tactcaaatt agtgatttca ttgaccttag gaataatata gtaaaggaat
    30421 tatgtttttt aactgacact aaggaaaaag attttttaga ttatctagaa aaaaatagca
    30481 cactctaata acctaaacct ttttaaactt taatatataa atacctgatt tagaatttat
    30541 ttttatgctt aatatattat cattaaaggt tttcgacata actgttattt gattaaatat
    30601 agggtctact atagtattac cttcttcaag tcttataaga cttcttgggt ctatgctttt
    30661 attatatgcc ttaactccaa ttttatattt tctcaattta acaacatatt cggacataga
    30721 aaaaataaaa tctatattat cttcatttaa tttactatct tcacccaaaa tggataatat
    30781 agattttttt atgctattaa ttccttcatt ttctacagtt ctatcttcca tatatacatc
    30841 atatagaaca tttaaaacat ttaataaaat actttcttca tcataatctt cagatttcaa
    30901 atcaatgtac ttatcaatta attgtattgc tttaatcctt gccatctgaa actttacaac
    30961 atctgatttg tctaattcta tagaaaatcc tattatcttg tctttaatac tctgtagtaa
    31021 ttcgacatac tctattttac tattatttat aagattgtgc ataatggaat tatacaccac
    31081 agaacctaat aaataatcaa ataaaagttc ttctttttta aaataaataa catacttttt
    31141 tattagttta ataacctcat cttcactttt ttgaatatta gtatttgata aaattagtgg
    31201 taataaaata tttaaaaaat tgatatttcc tattttttct ccataaaatc tatagtaaat
    31261 aagttcatct ctcagtctat tttcgtctat atacacacat ctttgcgttt tatcatctat
    31321 taaggatttt gtatttatta tagttaaatc tttaagagcc ctacttgcac tactaaaaga
    31381 agatgtgtag acttttttta gtaattcgtc attcataaat atatcctttc tgaaaaattg
    31441 aatgtaaata aattgttatt aaaaaaaaag agacagcccg tttaagaagc tgtcatatat
    31501 agggggatag tagtgtacaa aaatcattgt aatctactat ctatatatta ttatacgata
    31561 ctactttttt atatgtaaag catcagtttt cgacatccct atattttaca gttatacaaa
    31621 ttgcgtacta agttcaattt tattcttttt atttcaacat tttatcatgc tacattcttt
    31681 caggagcgtg cattcccaac aatttcagag aattttctaa agtttgtttt gttgctaaaa
    31741 ctaatgcaag tcttgctttt cttatttcca aattttctac taaaattgga ttgtcatgat
    31801 aaaatttatt aaacgcttga gctaaatcta gcataaatct agtcactata tgtggctcat
    31861 ttttctttag agcaagtaaa atattcttat tgaatgattc tataactcta agaacttctg
    31921 cactatcttc atcagctaat agactataat ctatatcaga agtcacttct acttctgctt
    31981 ttcttaaaac tgcacagcat ctagcatgtg tatactgaac atatggtcca gtctcaccat
    32041 caaaactaag cgttctttcc caagagaagg tatagtcttt aattctacta ttagataact
    32101 cttggaatac tactgcccca acacctactt gcttagatat ttcatccaca tttttagcat
    32161 ttgggttttt agctagtata atttccttag ttttatctat agcttgattt aaagcatctt
    32221 ctaaaaatac tactcttcct tttctagtcg acatagtacc ttcttcaagt gcaaccatac
    32281 caaatccaac atgaattaaa tcttttgccc aatcatatcc cataagctca attaccttaa
    32341 accattgttg gaaatgtaag ttttgttgag aaccaactac atatatacac ttgtcaaaat
    32401 cataagtttc ttttctatat attgcagcag ctaaatctct tgtcatatat aacgttgaac
    32461 catcattctt agtaataagt gctggtggca ttttatactc ttctaaatca acaattcttg
    32521 ctcctttaga ctcttttaaa agatttttct cttctaatag ttcgataact ctgttcattt
    32581 tatcagaata aaaactctct cctgctaaag aatcaaattc tatatctaaa agtttataaa
    32641 ctctgttaaa ctcttttaaa ctctcatttc taaaccattg ccataattct tgtgcttctt
    32701 cgtcaccatt ttctaattta ttaaaccatg ctcttgcttc atcttccatt tctgggtgtt
    32761 gttcagcttc atcatggaat cttacgtata attttagtaa ttctggaatt ggattagact
    32821 ctactacttc tttttctccc cactttttaa aggccactat taacttacca aactgagttc
    32881 catagtcacc taaatgattt attcttatag tcttgtatcc ttgcgaatca tatatcttat
    32941 ataaagcatt acctataact gtagttctta tgtgccctat atggaaaggt tttgctatat
    33001 ttggagaaga atattcaact attacagttt tatcttttcc aaaatcacta tgtccgtaat
    33061 tttctttttc atctaaaact tttttaatta cagtttctgc taattgagat ttatttacaa
    33121 agaaatttac atatccgcct aattgtatta cttttgttat ctctccactt ggttctatac
    33181 tttccgccaa ttcacttgct atcatgtttg gagcttttct aaacactttg gctaatttaa
    33241 agcatggaaa agcatagtcc cccatatcct tatttggtgg aacttctata agtgcctcta
    33301 tctcttcttt cgataaatct tctatttttt ctttaaggca atttgatatt gccaccttaa
    33361 aatcttgcat tctgttgctc ctctctacat tgttcctggt ttatattgtg cttatctctt
    33421 tatgttaaat caaatttcgt gttttttcaa gtatttcatt acagtgtaaa acactttagt
    33481 aattatgttt tctgtatata ttaaaaatac tataatttca gtttttaatc acactattat
    33541 tgatttttta taagactttt ttctatttta ttgtttcttg tactatccag ttgactttat
    33601 atactttttt taatctttta tctgttttca tacatcaatg tcagctttat atacattttt
    33661 ttaatctttt atctattttc atacatcaat gtcagcttta tatacttttt ttactttttt
    33721 atctattttt atacatcaat attagcttta tatacttttt ttactctttt atctattttc
    33781 tataaatctt tttcactctt aacttttatt ccttcttctt ctaatagcct tgctgttata
    33841 cctttccctg gtatcttttt acctgaaaaa ctaccatcat atattagatt acatccacaa
    33901 gaggggctac cctcttttag tattgcttcg ctacatacat ataaatttgc tatttttaga
    33961 gtttcattag cccctcttac aaagttttct gttacatcct tattatcttt agtaattact
    34021 tttccatcaa ctatctcagc aggtggtctt ggtgtagata gaccacctaa ctgctctggg
    34081 catacaagtg taaattcttt gccttttaaa tattccttga ctttttcatt atcattatta
    34141 tcgccactat atttacaatt tattcctatt aaacatgcag ataccaatat cataaaaatg
    34201 tgctcctata caaattatat atattctcat acaaattact aatgtattct tatacaaatt
    34261 attttaattt aacaattgtt actcctgctc caccttctcc atattgacca cctctttgtg
    34321 attttacatg tctatttctt cttaaaatgt cttgtagccc tgcttttaat actcctgtac
    34381 ctattccatg tattactgtt acagactcta aacctgccac atatgcatca tcaagatact
    34441 tttctacttc cattatagct tcttctaggt ttaaccctct aaggtcaact tcatttttta
    34501 cactaccaga ttttgacctt ataatgtttc tcgttgattt agttacattt gttgaaacat
    34561 ctttgcgtgt tttttgtaaa gatttaaaag gtagagtcat cttcattata ccaatttgca
    34621 ctactgcttc ttttctcttt ttatctactg aaactacact accattttga tttaaagtta
    34681 ttaccttaac ttcttcacct ggctttaagt cttttatctc cttatttgat acctttggaa
    34741 ctatcatact ttttactgtt ggctgaagac ttcccataga gctagtcaat tcttttctaa
    34801 gttcttctat cttacgattc ttttcttttg aagctctttc ttgttcaaga cttctaagtt
    34861 cttttattat aatatctact tcttctttag cttgtcttat aattgaaaat gcttctgatt
    34921 tagctttttc aatcatctta tctctttgac tgaccaattt ttcaagtttt tcgtcatact
    34981 ctactttcag tttttcaatc tcttctttta atctttcagc ttcttctcta tcttctactg
    35041 ctttgattct attcttttca acattctgta acacatcttc taaagctatg ttttctgtat
    35101 ttatatattc tttagcacgt gaaatcacat aatcacttag cccaagtttt ctagaaatct
    35161 caaacgcatt tgattttccg ggaacaccta taagtaactt atatgttggg cttaaagttt
    35221 ctatatcaaa ttcaactgct gcgttttcta cacctggttt agtaagagca taatttttaa
    35281 gctcactata gtgagtggta gctatacatt tagctcctac agaatttata tcttctaata
    35341 ctgctattgc aagggcagct ccttcaacag ggtctgttcc tgctcctaac tcgtcaaata
    35401 taactaaaga gtctgctgtt acgttttgta atatagatac tatattcgtc atatgagatg
    35461 aaaatgttga tagactctgt tcaatactct gctcatctcc tatatctgca aatacattat
    35521 catatacaca catactactt ccataatcag caggtatatg aagtccactt tgtgtcataa
    35581 gtgcaaatag acctacagtt tttattgtaa cagtcttacc acctgtgttt ggacctgtta
    35641 tcaccaatgt atggaagtct cttcctaaat aaatggtatt tgctactacc ttctttttat
    35701 ccagcagtgg atgtcttcca ttttttatgt ttaaatattt atcctcattt aaagttggtt
    35761 ctattcctct catctgtata gaaagttttc cctttgaaaa tgcaaagtcc aatcttccaa
    35821 gtatctcttg attagagatt aaatcttcgc tcacttctcc aaccatagca gaaagctctg
    35881 ataaaattct ttctatttct tcttgctcac ctagttttaa ctgtctcaat tcattgttca
    35941 tctctactat agtcattggc tctataaaaa gtgtcgcacc agaagatgac tggtcatgca
    36001 ctataccagc tacttgcgac ctatattcag attttacagg tactacaaat ctatcacctc
    36061 ttagagaaat tattgcatct tgtaagtact tttgataagt agttgaactt attattgaat
    36121 ttaattttga cctaatggat tgattttttt gggctattct tcttctgata tctctaagtg
    36181 tacttgaagc attgtcagat atttctactt cactaattat agcattgtat atctggtctt
    36241 ctatgtcctt gtatacatat agagaatttg atagagattg tattattgga taattaaaat
    36301 cttcttcatc actactagat aagctatttc ttaatcttct agccactctt aaggtatctg
    36361 ctatcataat cagacttcct gggtcaagag aagcacctat ataagctctc tttaccttat
    36421 cttctatatc atgaatacct tccaacccta ctgaacctct ctttataata attgattgag
    36481 cttcactagt ttcttctagc atagatttca cttctacaaa atcagtattt ggtactaaat
    36541 tttctatata ttttagacct aaagaagatg aggctttctt ctttaacaaa tctattattt
    36601 tattgtattc tagaactctt aacgatttct cattcatttt ctatattcac catcctcgat
    36661 attttaactt ttttatttaa ttttaaaaat gtatgttttc aagcatttcc aataaaaact
    36721 gcaagtttta tttctttttc atttctaaaa ttattatatt ttttctcttt tagatatatt
    36781 ttacacatat tttgtattat actatgtatg tagctatcta tatattatac ataaacatgc
    36841 tttatttgta atataatctg ttaaaaaatc ttctttaata tttttaaagg agtttatttt
    36901 agcttataaa aaaaaatagc tacttatata gataaattaa ggaggaacat gagtcatggg
    36961 aaacgtatta gaaggattaa aaccagaaag tgtttttaaa aactttgaga aaatatctca
    37021 aattcctaga ggttctggaa atgaaaaagg cataagtgat tttttactta gctttggtaa
    37081 aaatttaggt cttgaaacta ttcaggatga aagcttaaac ataataataa gaaagcctgc
    37141 tacaaaagga tatgaaaatt gtccaggtgt agttcttcaa ggacatatgg atatggtttg
    37201 cgaaaaagaa aagaatgttg aacatgattt tcttaaagac cctataaaat taagaattga
    37261 tggtgacatg atatatgcta ctggaactac tctaggagct gacaatggta tagctgttgc
    37321 tatgggtatg gctatattag aagataatac tttagagcat cctgcactag aagttttagt
    37381 tactgttaat gaagaagatg gtatgaatgg tgcagatgca ctagacccta gcttaattaa
    37441 aggtcaatat atcctaaata tggactccga ggaggaagga tacttattag ttagctgtgc
    37501 tggtggtaaa acttgtgttg ttagtttacc tgttgaatac aaggaagtta aaggtgacaa
    37561 acaaggatta ttagttgagg ttactggact attaggtgga cactctggta tggaaattgt
    37621 tttacaaaga gcaaatgcta acaaagcaat agctagagta ttaagtgtat tgaatgttga
    37681 ttatgaatta gcttctgttg atggtggtac taagcacaat gctatacctc gtgaagctaa
    37741 atgtgtaata gctgttaata aagctgatgt tgaatctgct aaaaaacaaa taaatgacat
    37801 tttaactgca tttaaacatg agtttactac ttctgaccct ggcatgactt actcagtagc
    37861 tgaaacttct gtagacaaag ttcttactaa agattgtaaa gaaaaagttg ttcaaatgtc
    37921 ttgcttaact cctcatggag ttcaatcagt aagtcttgat atagaaggat tagttgaaag
    37981 ttctactaac ttcgctataa tcgaaactaa agaatctact attgaatttt taacttctgt
    38041 tagaagttct gttatgtcta ttagagatga aatcgctgat agaataagac ttttggctca
    38101 agctttaggt gctaattacg accttatagc tcaataccca gcttgggaat ttaaaaaagg
    38161 ctcaaaatta gaaaaaatat gcagtgaaac ttatgagaaa ttaactggta aagttcctac
    38221 tgttatggct ctacatgctg gtcttgagtg tggattatta cttgataaac ttcctcatgc
    38281 tgaagctatc tctataggac cagatatgtt tgatgttcat actccaaatg aacatgttag
    38341 tatcccttct gtagctaatg tttgggatta tgttattgaa atattaaaat ctatgaacca
    38401 atattaaatt taagttttga ttctaagtag attttaattt taagtaatct atataaaatt
    38461 aactctgtaa attaatacta cggttagtat aatatttact ttcaatatta acttatagaa
    38521 ataaagccta atgatagata ataacatgta tcattaggct ttattgtaat ttaaatatta
    38581 ttaagcttta ttctgtttta aaattaattc ttatactatt ttaaatctat ttcttccttg
    38641 tctcttagaa tcataaagag cattatctgc tatttgatac agttcatcat actcttgacc
    38701 aggctttgct ataactaccc ccatactaat agataaactt tctccatttg gcaacttaat
    38761 gagatgaata tgattgtata catcattaca tttattaata agttcttcct tatttattat
    38821 atcattgata taaacactaa attcatctcc acccaaacga cctactatat cattttttgc
    38881 aaaactttca tcaagtgcct ttgcaacatc aataagtact ttatctccta acaagtgacc
    38941 ataagtatca ttaatcgatt taaaataatc aatatctata atcaataaag caccattttt
    39001 ttcagttgat aaattcaaca aattttgtga agttaatttt ctaatatatg ctgcattata
    39061 aatattggtt aaactatccc tttgagcctt atctaacaat ctattctttt cctctttttc
    39121 agaatcaata ttacatattt tcccgattac atacattggt atttttctat cattataaat
    39181 tatttttgaa gtaactctaa tccaatgaaa agtatcatca agcataatag aatacatttc
    39241 agttgttcca ttttcttgtg attttattac atcaaaaaac tttttattat attctttcct
    39301 tattttatct ctttcatttt ctatattgcc attttcactt tccttatcat ttttatccaa
    39361 accataataa gtctcaatac ccttttctgg catagaaatt atcaatttat cttgcttata
    39421 atcatactca aaaaagtatt cacttgtcat ttcatacaac tgagtatgtt ttttgagttc
    39481 taaagatatc ttctcgttca tacttgtacg agtatgtaaa ccaaatgcca taaatccaat
    39541 aatacaaatt aataaactgg ctacaataaa aatagtctca attggattct gttgcataat
    39601 ataaagcaac gagaaatctt gtctatatgt agtattttgg tagacaattg attgcatttt
    39661 ttctgatgaa atacttataa tagctttatt aaatatattc aataattctt gtttatcttt
    39721 tttaattata ccaaaacaaa tactctttgc tttataggtt tgaggaacta gacgcacatt
    39781 tttatactta ggttgattca tatagtactg catagaataa ccttcgccat aagtgtaatc
    39841 tgcatagcct ttattaacag cttctataca ctcttctaaa ttatcatacc aaactatatt
    39901 ctcttcaaac tgaccataat aattaatacc tttggctaga gctacacgtt ttccatctaa
    39961 actattctca tttaatttat tattaatcat catagtatat tgagtagaaa gaaaaggccg
    40021 agacatagct atattatgtt cacgagagta ttcataatca taaatcattc ctgctacaat
    40081 atcaatttca cgctttttag tcatgttttc taaatcttct tgagatttta caggcacaag
    40141 ttcaaattct aagcctgttt tatcacatat atgatttaac aaatcaattg atatacctct
    40201 taattcacct gtatctttat ctatattttg aaatggtggt ttgtttaaaa gtactcctac
    40261 ttttaatttt tctgtctctt taatgtaatt agtttcttca tcagataaaa acatttgatt
    40321 attctctggt gtaaagtatt tttcatataa agtagttgaa aaatatgggt ctttctgttc
    40381 aatattatta attgctgagt taagcttttg agttaaattt gtactacctt taggagtaat
    40441 gaaataaaat ggttttgatg aaaattttgc tacagtacgt acaccttcta ttgggttcat
    40501 actcacatta agaagtacat ctgctttacc actctttagt gcttctagtt gctcatcatt
    40561 tgttttacaa tataccaatt caggtgacat aagattcatt ttacaatact catctaattc
    40621 tttaagtcta gtatcagaat tttctattac agcaatacgg atttttttca catcttgtga
    40681 actcattatg ttatcaatat ctgtattttc atataatgct tgtagtactg tatttgctac
    40741 tccataatta ttccctgcat aattatatat tttttgcatt ggttcattgt ataacatacc
    40801 tcccattata tcaatttcac cattttctaa catcttcatt aattttgtaa tagattcatt
    40861 tatctctcca ggaacttgaa caaattcata attccatcca gtatactgtg caatttctag
    40921 taaatattca tatgtatacc caaaatagtt accttgctca tctatttcag ttaaaccttt
    40981 ttgaatagga taagcaacac gaatggtacg attatcatta cctatagcgt tgataccaaa
    41041 agaaaatatg caagaagtga atatagatgt aattacaata attaataaaa gtaatttttt
    41101 taaattcact acacaatcct ccatatagtt aagatttaca gtataaatta ctgcataaac
    41161 ttgtagacat gtaaaaatag attagtatat aaaaggatat ataaatctac ttagaaattt
    41221 aaatatcctt aatatataca atatatttaa attataactc atttatatgt ctattacaac
    41281 aaatttaccc tgctaaatac ctactctata tcccataatt ttacaagttg tagtgaagtt
    41341 taagttctat ctcacctcaa atttagcagt gtttctattt acagttcttt tatatttcac
    41401 atctggataa cccaatataa atgaagtgat aactgcttcg ttctcgctca ttcctaacag
    41461 ttcttttatc tttggctcta catttgatgc cataacaaaa aatccattat aacaaattcc
    41521 taatccttga gaataggcca taagttccat attagatgca gccaatcctc catctacata
    41581 agcacttcca cctagagaca tatcacctac aactaccata agtagtgggg catcataaaa
    41641 taaagaatca tttccgttct ctttgtatct tttgtacatt ttcttaaatg tatttttata
    41701 gatagaacgt acagggtcat tgtcatcagt atctaatgcc aaattataaa gaccttgtat
    41761 tgccagttct ttcacttctt ttagcttttc ttttactaat atgtatctta taggctgtct
    41821 attaccacct gttggtgtgt atctaccagc ttccactatg ttttttattt tttcttcttc
    41881 tacctctata tctttatatt gtcttatact tcttctaaat ttaataaaat tcaataactt
    41941 ttcgggttct aattcaaaac tttcacgatt gtattctatt acttcatttt tatcaaatcc
    42001 ttgcatttct attgcatctg ttgggcaaat tgccatacag tgaccacagt taatacagag
    42061 aatattttta acctttgctt tatcatcctt aacttttatt gcatttacaa gacagtctga
    42121 ttcacacatc ccacagccta cacatttttc taaatttatg tctatcattc caaattattc
    42181 ctccttttaa aaaatttact ttagtataaa acaaatagtt gtctaagtca atttttattt
    42241 ttttaacaat atactagcct taactacact ttaatggaga aaaatataca taaattggaa
    42301 cttaaattag aacttattca aagtaaaaga taaaagaagt agatgtctaa aagtgaactt
    42361 tttagttaat tagatattct ttattgtatg aaatcaagtg gatatttatc atttcaacaa
    42421 taaaaaaggc ttacctctat tttggatatg tccctgtcaa gtagacagac taaaaaaagg
    42481 ctatctttat gcactgagat agcccctttt cccaactact attgtttatc ttaatattag
    42541 tcaataaatt taatttgcta taacctgtaa gttacatttt aatttgatgt aactttaaat
    42601 tacattttta agtaatatct ttaaattata ttcttaaaat atatcattaa actacatttt
    42661 aattaatcta aaaactatat cttaattaat ctaagaaact tacattataa tcttttagtt
    42721 tcccattatc atatacaatc tttgcactaa agatatttcc agtcttagcc caatttaaga
    42781 acagcctgtc accttcccac atatttaaat cgaatatttt atcattatca acccaaatta
    42841 aatttccttc tttacaatca tttaaatctt tttcagataa ttcaccttca aactcatctg
    42901 agaaatacaa aaacatatac caatcttctt catctttaaa ttttggaaaa gttatcaacc
    42961 ctttatatga caatgattta agagtaagac cagtctcttc ttttacttct ctaattacac
    43021 attcttctgg agtctctcct tgctcaaact ttcctccaac tcctacatac ttaccttcat
    43081 gtatatcatc tttcttttta tttctgtaca acatcaaagt cttgtcatct ttttctatat
    43141 aacaaatagt tgtaagtttc atgtatctcg ccttgctttc tcttttatat tctcaactaa
    43201 ttattttcag cactttcaag cacatcactt atattcttca ttgcattaag agaaagctct
    43261 gcaaattcat caaatggaat ccctatatct tctatacttc tcattgcatc tctatttaca
    43321 tttgaagcaa atctcgtttc tttcattctt ttaataacag acttagtctt tacactggta
    43381 agttttttat ctggataaac taaagctata gcagttataa acccagttat agggtctgct
    43441 gcatatattg ctttttccat taggctagtt cttgtaactc ccattccatc atgatgacca
    43501 agtattgctc tatacatttc ttcgttaccg aagttttctt ttttaagcag ttctactgtt
    43561 gttgtagcat gtccttcaca tatttctcct ggctttcttc caactatatc tgaatccaaa
    43621 tcatgtaaaa gacctgccac tgcccactct tcttcttttt ctggctctag tcttttggct
    43681 aattctctca taacagcttc aacagcataa caatgttttc tcattctatc agtttctaaa
    43741 tacttaaata aaatttctaa agcttcttct ctcattttca ccaatccccc tgttttttca
    43801 catatatgat atttttatat attaatacac ataaattcaa gcttttatat atattcatgt
    43861 atatttttca tgttatatct attctacacc tttatagtag tgtcctgcaa ctgttccaat
    43921 ttcatctaat ttcttataca agtctgtagc cttttttcca agtttataat tattactcag
    43981 tacctcaatg tgagcttcaa taaccttttt aaccatctca ctatcttcaa ctgtaaaatc
    44041 tatcctaaat acatctattc caatttcctc taactcatat agattgtcca acatacaagt
    44101 aaccttcgaa ttgtaaattg tacttctaca gaatatatct tgtgttatcc tataacttgc
    44161 atccgtaaag tctcttaaag cataactact ttccttacat ttagcaactc tcttgtcctt
    44221 tttacaatct ctaacaacta ctcccatagg acagtattct gtaaccatca taggagcata
    44281 tccatatatt actgcttcaa tttctacatc tgtatatttt aaagtttgtt taatttcatt
    44341 taagtttagt tcttgtgaca aacaaactgt acttgctccc tcttctttga agtgatttat
    44401 actttcacta ttgaatacat ttaagtagca atctatattt aagtcttttt tcttaaagta
    44461 ttccatagag ccccagttac caatttgaac cttattaatt ccttttatat tatccaatat
    44521 ctcaaattgc ttgtactctt tatttctaac aatccttggt gctgaataga ttaatttttt
    44581 gtcagtgact gctgtattag agtggttctt attcaaagta tctattttac ttgtatttga
    44641 caaagtatct atgttatttg tatttgaacc cattagttct ttaggagtac ctaaagacga
    44701 agaagtatct aaatccatag aaatacttga atctatagaa atatttgaat ctatagagat
    44761 acttgagacc atttgtaaag cttttgataa tgtatttata tcttcataat agattaaatc
    44821 tacattataa tttaatactg cttctaattg ctccaagttc ttaactttaa ctctaatctt
    44881 aggagctgta tcaagttttt tctgtttatt tactctattt ggctggtatt ttaatctatt
    44941 cttcttaaat tttctatctt ttatctttat tctttcttca tctaaaagct caatacaatc
    45001 ccttctcatt tgatttaaca cacttatagg catacttaca ccttcatcca ggtcaatttc
    45061 tatctctttt aacttatatg gtgtatttcc aagcttttga atttgtgttt ctattttttc
    45121 tttacttaaa gccaccttta tagctttttc aactattttg tctccttcta ccaccgattt
    45181 atttccacaa aaatcactta taaaaagctc tggcttttta cctagtttaa tactcacttt
    45241 tgcacgtata ttagttttta tgaactcttt atcttgctga aatgtagctt gtactctatc
    45301 cattaattcg ctgtcagaag tcttatatat aagctgattt ctctttgctt ctccaacaaa
    45361 atctaattct atcaaatccc ctttatgtgc tattgtagtt atctctccat cctttattat
    45421 tcttccaata gtacctccac ctaagttaat tccatcacct ttcttaagcg tattttctag
    45481 cattactcta agtcttttgt tctttctatt ataatctaaa acttttccaa tatatagccc
    45541 ttgattatta ggtctattag agttcattat atccttacca acttcaccta atatatatcc
    45601 ttttgtaaac tttctattaa atatggtata caaattattt attatttcct cagaaatact
    45661 tatttttttc ttatctacaa attcattaat cgcttctcta tatcctgaaa ccacagtagc
    45721 tacatactct ggctttttca ttctaccttc aatttttaaa gataatacac ctgcttcaat
    45781 tattttatcc aaatcttcta tagtacttaa atctctagga ctcaacaaat aatctccatt
    45841 actttttact acttctcctg tatttatatc tatcaactca tacttttgtc tacatggctg
    45901 tgcacaccta cctctatttc cagacctatt gcctatcata ctactcataa ggcattgacc
    45961 cgagtaacat acacataaag ctccatgtac aaaaacttct atatctacag ttgtattttc
    46021 acaaatccat tttatctcat caacatttaa ttctcttgct aaaacaactc tatcaaaccc
    46081 tacactctga aggtatttta catcttctaa ggaatgtgct accatttgag tgcttgcatg
    46141 tagttcaaag tcaggtaata gtttttttat aagtcttgcc attcctatat cctgtactat
    46201 taaagcatca atatctatat cgtataaaaa ttttacatac tctacaaaat cttctatttc
    46261 atactgtttt atcaatgtat ttacagtaac aaagacttgc actcccctta tatgagagta
    46321 tttaacagcc tcaatcagct catttctatc aaagttattt gcagatgctc tagcactaaa
    46381 gtctttacca cctaaataca cagcatttgc tccgttttga actgctgctt ttaatgaatc
    46441 aaaagagcct actggtgcaa gtaattctat ttttttcata tttttctcct ctatttatac
    46501 ttgttaggtt aatataatta tatctataac ttaaatattt gtattaaatt tatatatttc
    46561 ttactatatt ttaaattctt caatcaattt atatattagt tatttatatt tggttcattt
    46621 ga
//
LOCUS       DAMJWU010000021        45601 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMJWU010000021 DAMJWU010000000
VERSION     DAMJWU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.021"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..597
                     /locus_tag="PC460_001318"
     CDS             <1..597
                     /locus_tag="PC460_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HDF2600211.1"
                     /translation="TGATGVTGATGPTGPTGATGATGATGASAIIPFASGIPLSLTTI
                     AGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITSISAYFST
                     TAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGIVTGL
                     NIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            766..1527
                     /locus_tag="PC460_001319"
     CDS             766..1527
                     /locus_tag="PC460_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2600212.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1836..2114
                     /locus_tag="PC460_001320"
     CDS             1836..2114
                     /locus_tag="PC460_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600213.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2407..2982
                     /locus_tag="PC460_001321"
     CDS             2407..2982
                     /locus_tag="PC460_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF2600214.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(3088..3921)
                     /locus_tag="PC460_001322"
     CDS             complement(3088..3921)
                     /locus_tag="PC460_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF2600215.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(3989..4618)
                     /locus_tag="PC460_001323"
     CDS             complement(3989..4618)
                     /locus_tag="PC460_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF2600216.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            4929..5588
                     /gene="cprR"
                     /locus_tag="PC460_001324"
     CDS             4929..5588
                     /gene="cprR"
                     /locus_tag="PC460_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF2600217.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            5799..6857
                     /locus_tag="PC460_001325"
     CDS             5799..6857
                     /locus_tag="PC460_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2600218.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            7108..9252
                     /locus_tag="PC460_001326"
     CDS             7108..9252
                     /locus_tag="PC460_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7522..7524,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HDF2600219.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            9301..10134
                     /gene="fdhD"
                     /locus_tag="PC460_001327"
     CDS             9301..10134
                     /gene="fdhD"
                     /locus_tag="PC460_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF2600220.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            10145..10318
                     /locus_tag="PC460_001328"
     CDS             10145..10318
                     /locus_tag="PC460_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600221.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            10376..10927
                     /locus_tag="PC460_001329"
     CDS             10376..10927
                     /locus_tag="PC460_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2600222.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRQCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            10952..12328
                     /locus_tag="PC460_001330"
     CDS             10952..12328
                     /locus_tag="PC460_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2600223.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            12328..12843
                     /locus_tag="PC460_001331"
     CDS             12328..12843
                     /locus_tag="PC460_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2600224.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNLDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(12921..14204)
                     /locus_tag="PC460_001332"
     CDS             complement(12921..14204)
                     /locus_tag="PC460_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF2600225.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            14392..14814
                     /locus_tag="PC460_001333"
     CDS             14392..14814
                     /locus_tag="PC460_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF2600226.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            14916..15854
                     /locus_tag="PC460_001334"
     CDS             14916..15854
                     /locus_tag="PC460_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF2600227.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            16040..18064
                     /gene="ligA"
                     /locus_tag="PC460_001335"
     CDS             16040..18064
                     /gene="ligA"
                     /locus_tag="PC460_001335"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF2600228.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            18224..19573
                     /gene="rph"
                     /locus_tag="PC460_001336"
     CDS             18224..19573
                     /gene="rph"
                     /locus_tag="PC460_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF2600229.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKIIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            19573..20043
                     /locus_tag="PC460_001337"
     CDS             19573..20043
                     /locus_tag="PC460_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2600230.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            20237..21523
                     /locus_tag="PC460_001338"
     CDS             20237..21523
                     /locus_tag="PC460_001338"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF2600231.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            21673..22257
                     /gene="clpP"
                     /locus_tag="PC460_001339"
     CDS             21673..22257
                     /gene="clpP"
                     /locus_tag="PC460_001339"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2600232.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            22276..23526
                     /gene="clpX"
                     /locus_tag="PC460_001340"
     CDS             22276..23526
                     /gene="clpX"
                     /locus_tag="PC460_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF2600233.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(23597..24124)
                     /locus_tag="PC460_001341"
     CDS             complement(23597..24124)
                     /locus_tag="PC460_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2600234.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(24112..24624)
                     /locus_tag="PC460_001342"
     CDS             complement(24112..24624)
                     /locus_tag="PC460_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2600235.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            24804..27167
                     /gene="lon"
                     /locus_tag="PC460_001343"
     CDS             24804..27167
                     /gene="lon"
                     /locus_tag="PC460_001343"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF2600236.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            27157..27768
                     /locus_tag="PC460_001344"
     CDS             27157..27768
                     /locus_tag="PC460_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF2600237.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(28034..29404)
                     /locus_tag="PC460_001345"
     CDS             complement(28034..29404)
                     /locus_tag="PC460_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2600238.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFVGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(29579..29694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            29982..31061
                     /locus_tag="PC460_001346"
     CDS             29982..31061
                     /locus_tag="PC460_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2600239.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            31453..32133
                     /locus_tag="PC460_001347"
     CDS             31453..32133
                     /locus_tag="PC460_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2600240.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            32126..33505
                     /locus_tag="PC460_001348"
     CDS             32126..33505
                     /locus_tag="PC460_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2600241.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            33676..34761
                     /locus_tag="PC460_001349"
     CDS             33676..34761
                     /locus_tag="PC460_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2600242.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            34815..35174
                     /locus_tag="PC460_001350"
     CDS             34815..35174
                     /locus_tag="PC460_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2600243.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            35227..36099
                     /locus_tag="PC460_001351"
     CDS             35227..36099
                     /locus_tag="PC460_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF2600244.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYISNIGMII"
     gene            36100..36543
                     /locus_tag="PC460_001352"
     CDS             36100..36543
                     /locus_tag="PC460_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600245.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSRHTLPTTDKTREKFSKIKLNEIKKIYETIGINIITKISIDK
                     NKLEIEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            36558..37142
                     /locus_tag="PC460_001353"
     CDS             36558..37142
                     /locus_tag="PC460_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600246.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANLLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            37395..38432
                     /locus_tag="PC460_001354"
     CDS             37395..38432
                     /locus_tag="PC460_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2600247.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            38513..38878
                     /locus_tag="PC460_001355"
     CDS             38513..38878
                     /locus_tag="PC460_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600248.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            38883..39200
                     /locus_tag="PC460_001356"
     CDS             38883..39200
                     /locus_tag="PC460_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600249.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            39248..40852
                     /locus_tag="PC460_001357"
     CDS             39248..40852
                     /locus_tag="PC460_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600250.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            40976..42604
                     /locus_tag="PC460_001358"
     CDS             40976..42604
                     /locus_tag="PC460_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2600251.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTSLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAALDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNIYFSEEGQSELD
                     VMYNRVVSAYTYALESMRSDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            42869..44218
                     /locus_tag="PC460_001359"
     CDS             42869..44218
                     /locus_tag="PC460_001359"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF2600252.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            44374..45453
                     /locus_tag="PC460_001360"
     CDS             44374..45453
                     /locus_tag="PC460_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2600253.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT        18546 a         4912 c         8110 g        14033 t
ORIGIN      
        1 acaggggcaa caggagttac aggagcaaca ggcccaacag gcccaacagg agcaacagga
       61 gcaacaggag caacaggtgc tagtgcaata ataccttttg catcaggtat accactatca
      121 cttacaacta tagctggagg attagtaggt acacctggat ttgttggatt tggtagttcg
      181 gctccaggat taagtatagt tggtggagta atagacctta caaacgcagc aggaacattg
      241 actaactttg cattttcaat gccaagagat ggaacaataa catctatttc agcatacttc
      301 agtacaacag cagcactttc acttgttggt tcaacaatta caattacagc aacactttac
      361 caatctactg caccaaataa ctcatttaca gctgtaccag gagcgacagt tacactagct
      421 ccaccactta caggtatatt atcagttggt tcaatttcta gtggaattgt aacaggatta
      481 aatatagcag caacagcaca aactcgattc ttactagtat ttactgcaac agcttcaggt
      541 ctttcattag ttaatactgt agcaggatat gcaagtgcag gaattgcaat aaattagatt
      601 agataagaaa aggcttctaa tatcgtattg atattaggag cttttttaag aatgattgat
      661 gaacatagtt ttaatattaa ttagtgcgca tattataaaa tactattata taatataaaa
      721 taataacatt aaattggaaa tatagaatta tctaggggtg atactatgaa aaataagatt
      781 gaaatatcaa tgaacaactt tgtaaaaaat tatcataaat caaataatat aactacaaag
      841 tggcaagaac ttttgtttgc ttatgcatct gctgaagatg atatgtttca gaagctaaaa
      901 agtgttgtag gaacatctca tgctatgcca aaagattttc tggaaaatgg aaaaactata
      961 attacatatt ttataccttt tgatgaaagt gtaaataata gtaatattgg agggtttgaa
     1021 tgttctgaaa tatgggcaag agcgtacata gaaactaata tgttgatttc aaatttaaat
     1081 gatttcttat gtgaagaact agagaagata ggggctaatt catatgctat tcctgggtcg
     1141 tataattttg acagtgaaaa attggtaagt gactggtcac aaagacatgt agcattcata
     1201 gcaggtcttg gaacttttgg tttaaataat atgcttatta cagaaaaagg gtgttgtgga
     1261 agaataggaa gcataatcac aagtttagaa ttagagccaa ctaaaagacc agaaaatgaa
     1321 tactgcttgt ataaacataa taatagttgc aaaaaatgtg tcaataaatg cgtgaaagaa
     1381 gctttaaaag aagatggatt taataggaat aagtgttatg aaatgtgtat gataaatgaa
     1441 aaaaaataca cttatatagg tggatgtgat atttgtggaa agtgtatagt tggagttcct
     1501 tgttcagtaa taaacccatc aagataatgg atttaagttg actaataatt ttaaagatag
     1561 tttataatga aaaaatttta gttatcaaaa taaatataat ataaaaacta aataaatgtg
     1621 tttaaaaaat ggataaataa tttgtgttga ttgacaacca gttatactgt tgatagactt
     1681 atatgtatcg ctgtgatata taaggagggt tgagatttta tgtttgattt aatctttatt
     1741 tttgcatagc aagggctatt gcaactatga aatttaatat ataaatagcc ctttgctatt
     1801 cctaatgaaa aattactatt taataggaga gcaaaatgag ctataaaaaa gcaatacata
     1861 tattaccaga agaattactt gaattaatac aggaatatgt agatggtgaa tgtatctata
     1921 ttcctagaaa atctaataat aaaaaagaat ggggaagtaa tacacctact cgtgaggagt
     1981 tgtcaattag aaatatgcag atttacgaag actatcaagt tggatatgat ttgcaatatt
     2041 tatcggaaaa gtattattta tctttgaaaa gtatccaaag aattgtatta caagaaaaaa
     2101 gaaaagatat ctaaggatga gccagtatag ttttataatt atactggctc attttctttt
     2161 gataaaagtg tttgaggtat agtttctttt aagtggatga tataatttaa gtaatttcaa
     2221 taacttatac agtagctaac aaagctggtt gtaaagtatc taataagaag tagtagcttt
     2281 ttatatgttt gaaattaaaa tttattaaaa ttaggaggtc ttgttttgaa aaaaatactt
     2341 gattatattc caccttttca taactttcta ttaatttagt aatcttatag aaaggaaatt
     2401 gcatttatga aaaaaattac ttgggaggtg gaatatatct cccagttacc tgtaattagg
     2461 tattgtaaaa aatgtggaaa aaagactgaa tatatttgtt caggtttatt tagggttaat
     2521 gctcaacata aatatttgga tatatggtta atatacaagt gttcaaattg tgattcaaca
     2581 tggaactcaa ctatatattc tcgtgttaat ccaaaaagct taaatccaga aatactagaa
     2641 caattccata caaatgatta taatcttgtt aagcaatatg ctatgaatat ggaactttta
     2701 catagaaatg gagcagaagt tggtattcca gagtataaaa tagtaggaca agaaattaat
     2761 attaatgacc ttacacaagt tcatattatt agtaaatatc catgtcagtt aaaggtatca
     2821 acattattac gtgagaaact aggtttatct aggaaggtat ttgacgaaat gttggattgt
     2881 gggattatta gaagtaactc aggagttgat ttaaagaaag gtaaattaaa ttatgaaatt
     2941 atgttagata taggcaagga gaagcaaaag tcttcaaatt gattagaata actaacttaa
     3001 tatattttca ggaacatcat ttaaaaaaat agtgacttta aaaaatttaa agccactatt
     3061 ttttaatatg ttttatctat ataactatta tttttctagg ttttttaata tttcgtaata
     3121 gtgtgttgtg gctatttcac ttgactcgcc ttttttacaa aaagataatt tagatagttt
     3181 tggaactaca cgttcaacga caattttatt acctatcatt ttagattcaa gtatcttcaa
     3241 tgcttctaaa aatctcttat ccttttttgc acgattataa aatgataaaa cgtagacata
     3301 aaggaatagg ttataattac ggaaaggata ttcaacttgc ataaataatg tgccaattcc
     3361 atagtgacaa gggccgattg gttttttaat agtccaatgg tcaagtagaa aatctacagc
     3421 tttgtcaagt tttgattctt tatttagata attactaaaa cggaatgcat ttaatatatt
     3481 aagagttggt agtggatttg agtattcagt ttctggacct cgaccaaaac taaatttatt
     3541 gcaacgccac ccaccatcag tatactgtgt atctaaaaaa tgttgaaacg ttttttgcat
     3601 tctaatgtca gatgcatatc ccatatgaca gagtacgtta gcagcatttg cagtatgaca
     3661 agggtaaata cctccatttg gatatagttt gaaacgtcca tcttctcgcc atgtgctaaa
     3721 tatcaggtca gcacattctt taagtaaagt atcatcaggg ttcacaccta attctaatag
     3781 ataaagtaca ctatcaagag tagagaaagg agaacctttt attaaacgct tatcaggtgt
     3841 agtccaatag tctgcaccat tatcatacct gtgtgataat attatttcaa tatctgataa
     3901 agattgattt tctacattca ttttttactc catttcatat tatattctat taaatctatc
     3961 aaaaacttta ttataatatt atttatattt ataattgatt tattttattt aaaattgcat
     4021 ttatagctgt atcaggaaca actgctaaat cttctgcatt gaagtaataa actatattag
     4081 aatcaaataa tatatttgaa tttggaggaa attcatcatc tccaaaccaa agtataaata
     4141 ccatagatgt attacccata tatgtaaacc tataagctaa atctcccatg tttacttttt
     4201 cagctccaat tttctccata actaacttat atttgtctat ttgagatgta aaaacttctg
     4261 aaagcctata tagagtcctg tttaaaaaat ttggataata aacattgcct cctggtattt
     4321 ctttaaaagt tacatattta ttagttctag gaataccttt agcattgatt aaatatctaa
     4381 ctatcaagat tttaagctca aagttattgc aaaggctacc attactattc caaacttctc
     4441 cacttggata gtctataata tattgtctat caaaaaaatt gagtgtgaat ctatttaaat
     4501 cattattgaa aggtatatta cttaaattac taattgtatg agggtctaat tctttgagtt
     4561 tttttcgaac atagtcaaat ggaacattat aattattttt tgttatcttt tcatccataa
     4621 ttttttcctc caatttaaac aattaaaagt atatttataa aaagttttct tattattaat
     4681 tttattatat attcaaaata ttacaataat aaattgttaa ataaattttt atatttaagt
     4741 aaatacgtgt aaaatgaata taaagttttt gaaatataaa gtttttgtat ctaaaatatt
     4801 ttatttgtaa aatagtaatt aattattgta agctataata tgagagtatt atggttaatg
     4861 tgaattttaa agatattatt aaatatataa attagaatac tattaaacgg ataaatggag
     4921 gagttattat gtctaagata ttagtagtag atgatgagtt agatatgtta aagttgattg
     4981 aaaatgtgtt gaaaagagat ggacatgaag taaaggtaat tagtgatgta gaaaaagcta
     5041 tgaacatgga ttttaattat tttaatctaa taatattaga tgttatgatg cctaagatgg
     5101 atggttttga agtatgtaaa actataagaa ataaggttga ttgtccaata ctgtttttga
     5161 cagcaaaaaa tatggaaagt gatattatgt acggtcttgg tataggtgca gatgactata
     5221 taaccaaacc ttttggtgta ggagaattaa gggctaggat aactgctcat ttaaggcgag
     5281 aaagtagaga aaaaaagaat ttattaacta tctcaaatgt tcaatttaac tttttaggca
     5341 aagaagtttc aattgatggt aaaaaaatta attttactaa aagtgaatat ctaatatgtg
     5401 aatttctagc aaaaaataag ggacaagtat tttcaaaaga aagaatatat gagtcggtat
     5461 atggttttga tggagaaagt gatataagta ctatcacaga gcatataaaa aatatacgaa
     5521 gcaaattaaa acaatatgat atttatacaa tagaaactgt atgggggata gggtataaat
     5581 gggtgtaata agtataagtg ttgtttagtg aaaatattat atatatctac aaaatgtttg
     5641 taaaatatta atctatatat taaaatttaa tttatagatt aatatttttt gtaaatgaca
     5701 aaattgtaag gtattttgtt ataaaatatt aagattcata gtgtatacta ataatatagt
     5761 atttagtata aatcttgagc aaaggatggt tatttctaat ggatagacta aagaatatta
     5821 gaaagagttt actacagtat acatttttat ttttgttaat aggtataaca atttacctag
     5881 tgtttaaaac attggatatt aaaatgctat caaatgttat ggttatggta gataagaagt
     5941 ttttattcat aggtgctttg gcaataatgt gtcacataat gcttgaagga gtagtaatga
     6001 agataattat agaaagcact cacaaagtta atattagatt tattggtttt aagctagcca
     6061 ttatgggatt ttattataat ttaataactc cttttgcttc aggaagccaa ccagtacaga
     6121 tatatgtact taaaaaatgt aaaatgccac ttagtaaggc aagtgcagtt gttaccaata
     6181 agtctattat cttccaaata gtagttactt tttattgtac aatacttgtt ttaatgaatt
     6241 ttgctatgtt agaaaaacaa atgaaaatga ttatgcctct agtgtttcta gggatagcca
     6301 ttaattcatt tgtcttaatt atgataatat tagttatttt aaatcctaaa aaaataaaat
     6361 attttatgag aattactata aagcatttat caaaaattaa gtttttaaaa tttttagata
     6421 ataagattga atccatagaa agttttatag atgattatag taaagcaata aatttttttg
     6481 caaagaataa aaaggtatta atttcaacta taatagtaac ttttattcaa ttaagtgctt
     6541 actttagtgt ttcattttgg atatataaag catttaattt acaaggttat agctatattt
     6601 atatactgac attacaagca tttttatata tggcaatatc tccaatacca acacctggga
     6661 atgtaggagc taatgaatta gcctttttca ctatattcaa atcagtattc cctcaacctt
     6721 tgatgggata tgcagtattt ttatatggtg gatttatgta ctatttgatt ctgattggaa
     6781 gtggaatatt tactgtaatt actcactata gaatgaaaaa tagaattggc agaaatattg
     6841 ttttaagtga aaactaaata agtttaaaga atatatattg agtatattaa aaaatatgtc
     6901 ataagaacta gaatatttta attaaatggt tgaaatataa agattattat gacatatttt
     6961 ttttgaaatt taagaggaat tgtgagaaaa ttgttgaatt taatagatag tattgcaaaa
     7021 tatttatgat tttgcatatt ataaaaacac tcattatctt taagataaat tttacttatc
     7081 aggggataaa tctaaggggg gaattgtatg gagaaaaaaa ttttaacagt ttgtccatac
     7141 tgtggagctg gatgccaatt atatctagta gtaaaagata ataagatatt aagagctgaa
     7201 ccggctaatg gtagaacaaa tcaagaaagt ttgtgtttaa aaggtcgtta tggatgggat
     7261 tttctaaatg accctaaaat tctaacacct aggctaaaaa agccaatgat aagaaaacaa
     7321 ggtgtactag aagaggttga atgggatgag gctataaact atacagcttc aagactaaat
     7381 gaaattaaag aaaaatatgg tcctgattct attatgggaa caggttctgc aagaggacct
     7441 ggtaatgaag caaactatat aatgcaaaaa tttatgagag ctacaatagg aactaataat
     7501 gttgaccatt gtgctcgtgt ctgacatgca ccatctgtag ccggtctggc ttactcatta
     7561 ggtagtggtg caatgtctaa ttctatacca gagatagaaa atgcagatgt tttatttata
     7621 tttggatata atggagcaga ttcacatcca atagtggcaa atagaatagt aaaagctaaa
     7681 aaaaatggtg ctaaattaat tgttactgac cctagagtaa ctgaatctgc aagaattgct
     7741 gatatacatc tacctataaa aggtggaaca aacatggttt tagtaaatgc ttttggaaat
     7801 gtattaatag aagaaggtct ttacaataaa gaatttgttc aaaatcatac acaaggattt
     7861 gatgagtata aagaaattgt taaaccatat acagctaaat atgctgaaaa gattacagga
     7921 atcccagaag agcttataag aaaagctatg agagaatatg caaaaggtaa aaaagctatg
     7981 atactttatg gaatgggtgt ctgtcaattt ggtcaagctg ttgatgtagt taagggactt
     8041 gcatcaattg ctctacttac aggtaatttt ggtagagaaa gcgttggaat aggacctgta
     8101 cgtggacaaa ataatgttca aggagcttgt gatatgggag ctcttcctaa tgtatatcct
     8161 ggctatcaaa atgtaactga tgataagata agagaaaagt ttgaaaaagc ttggggggta
     8221 aaattatctc ctaataatgg ttatagttta actcaagttc caaatttagt actaaaagag
     8281 aaaaagctta aagcttatta tatatttgga gaagacccag tgcaaagtga cccagatgca
     8341 tcagaagtta gagaagcatt agatgaattg gaatttgtca ttgttcaaga tatatttatg
     8401 aataagacag cacttcacgc tgatgttata cttccagcta cttcttgggg agaacatgag
     8461 ggagtatata catgtgcaga tagaggattc caattgatga gaaaagctat agagccacaa
     8521 ggagatgtaa aaccagactg gcaaattata agcgaaattt ctacagcaat gggatatcct
     8581 atgaattata aaaatactaa ggaaatatgg gacgaactta gacaattatg tccaagtttc
     8641 ttaggtgcta cttatgaaaa gatagaaact caaggatgcg ttcaatggcc ttgtaagagt
     8701 gaaagtatgg aagataaagg aactatgtat ctgtatgaag gtcaaaagtt ttctactcct
     8761 aatggtaaag gtaatttgtt cgctgctgaa tggagacccc ctatggaagt tgaagatgat
     8821 gaatacccat ttagtttatg tactgtaaga gaagttggac actattcagt aagaacaatg
     8881 actggtaatt gtagaacact tagttcatta gaagatgaac caggacgtgt tcaaataaat
     8941 tctaatgatg ctgaaaaatt aggaattgaa gatgatgaat tagttagaat tagttcaaga
     9001 agaggaagtg taataacaag agcaacagtt actgatagag ttaaggaagg tgcaacatat
     9061 atgacttatc agtggtgggt tggagcttgc aatgaactta caatagcaaa tttagatcct
     9121 atatctaaaa caccagagta taaatattgt gctgttaaac ttgaaaaact tgaagaccaa
     9181 gaattagcag aaaaatgtgt aagagaagag tatcaaagtt tgaaagacaa aatgacagct
     9241 acaaatatct aatgatttat tagatttaag atttataaaa ggcagaaagg ctaatatttt
     9301 atgaacaata tatactcaaa aactaaatta caagaaaata gtaaggataa agtttttgaa
     9361 aatagttata gtctagtgga ctacaaatat ttaaattctt ttaatgtaga agtaagcaaa
     9421 ataaatgaac atgaagtatc aatagataat gaagaaataa ttgcagaata tccacttagt
     9481 tttattttaa atgataaata tataaataca tttttatgta ccccatataa tttagaggaa
     9541 cttatagtag gattcttgaa aacaaaagga tacatagaaa acaaaaaaag cttattaaaa
     9601 tttgagataa atgagagtga acgttttgca aaagtaacta ttcaagagaa acaaaaaaat
     9661 caagatgagc aaattatatt tttaaattca ctagactata taacatgtgc tcctgtaaag
     9721 agcaatataa aaataaatta tgatacgatt tacagtataa tggatagaaa tttaaattca
     9781 tcagaattat ttaaaaatac tggtggggta catagcgtat ctgtatttaa ttttgataaa
     9841 aaaatagtca cttgtgaaga tgtagcaagg cataatgcaa tggataaggc tattggattt
     9901 tgtgttttaa atgacataac attaaaagat aaaattgtgg ctgtaagtgg aagaatatca
     9961 ttggaaatga tattaaaagc agctatgact caaattccta ttgtagtatc aaaatcagca
    10021 ccgactaatt tatcaataga gctttctaaa aaattaaata taacgcttat tggatttgta
    10081 agaggaagac ggatgaatat ttatgcaaat ccacagagag ttatcaaaaa gtgagaatga
    10141 atatatgtgt actaaaaaag aagagcaaag atattcaaag tgtgaaatct gtggagtatt
    10201 aattgatgat actgataagg tgtataataa cacatacaat ttgtgcaaaa aatgtaaacg
    10261 aaatataagt actgagaaaa tagtgaaata tataaatata tatgatgaaa gtaagtagac
    10321 atatgatgaa aataactaga gggttggaaa tatacaaatt gaaaggtggg ataatatgaa
    10381 aaataaaata ggctcttttg taatagcaga cccaaacaaa tgtacaggtt gtagagcttg
    10441 tgaagtagct tgttttacaa tgcataatca gcataataat gtgggttata cagttggaac
    10501 agttgatata cctgttatac ctagacttta cttagtaaaa ggtgacaatt tctgtatgcc
    10561 aatacagtgt agacagtgtg aagatgctcc ttgtttaaac tcatgtccac aaaaagctat
    10621 agttaaagaa aacaatatta tgtctgtaaa tgaagaaaag tgtataggat gtaaaacttg
    10681 tctgctagca tgtccttttg gagctataga cttacttcca caatatgaag atggtaaaga
    10741 agtagaacaa gtaattttag atgaaaataa gaagatagct tataaatgtg acttatgtaa
    10801 agacaatgaa aaaatagctt gtataagtgc atgtcctcag gaagcattaa aacttgttac
    10861 tccaatagat gacaaaaaag ctaagaatag acaagctgca cttagtttat tgttaacaaa
    10921 taaataaagg atacaggggt aggtgacgtt tatgataata aatatagata aggatttatg
    10981 tactggatgt agagaatgtt caaaggtctg tccagtaaat gctattgaag gtgaagaggg
    11041 aaagccacaa gagataaatt tagatagatg tgttatgtgc ggtcaatgtg ttcaaacttg
    11101 taaatcttat gcttcagtta tagatgaagg atttgagttt ttacaagaga aaaaacaaga
    11161 aagagagata ccagagtcta taaatgaacc tatatttgca gcatacaatg tatgtgatat
    11221 agataaggtg aagaaagctt taagtgaccc aaatgtattc actatggtcc aatgtgcacc
    11281 agcagttaga gttgctttag gagaagattt cggatattat ttaggatact taggtgctgg
    11341 gaaaatggca gcagcactta gagctttaaa ttttgataga gtctacgata ctaattttgc
    11401 agcagattta actataatgg aagaaggtag tgaattaatt aaaagagtga cagaaggtgg
    11461 aactcttcca atgtttacat cttgttgtcc agcttgggta aaattcatgg aaaaaaatta
    11521 tcctaaatta actaatcatt tatcttcttg taaatcacct cagcagatgg gtggtgcaat
    11581 atttaaaact tatggtgcag agataaataa tgtagatgca tcaaagatat ttagtgtttc
    11641 tataatgcca tgtacatgta aaaaatatga atgtgataga gaagaaatgg attctagtgg
    11701 atatagagat gtagatgtag tattaactac tagagaacta gcttatttaa taaaagacat
    11761 gggaattgat tttaaaaatt taagagaaga aaaatttgat agtcctttag gtagttatag
    11821 tggagctgga actatctttg gtgttactgg tggggttatg gaagctgcta taagaacagg
    11881 atatgagctt ataactggag aaagtattcc tgatgtagaa gtaaaacaag taagaggaga
    11941 aaatggtttt agaaagtcaa ctattaaaat aggagactta gacttaagag ttggagtagt
    12001 aagtggatta aaaaatgtaa taccagtgct tgaagacttg gaaaaaggaa aattagatgt
    12061 agattttatt gaagttatga cttgtcctgt tgggtgtgta agtggtggag gtcaaccaaa
    12121 aattttacta gaagaatata aggaattggc ttatgaaaat agaacaaaag caacatacgt
    12181 gcatgatgaa catttagcaa ttagaaaatc tcatgagaat cctgatatta aaaaactgta
    12241 tgatgaatat ctggtagaac cattaggaga aaaatctcat catctattac acactacata
    12301 ttgtatagga aaggaagtgg ctaagtgatg aactattttg ttatagcaga ccctgataaa
    12361 tgcataggat gtagaacttg tatgatagga tgtgtagttg cacattcaga tgaagatatt
    12421 ttttaccaaa atctagatga aataaatttt aatccaaaac tttctgtcat aaagacaaaa
    12481 gaagtaagtg ctccaataca gtgtagacat tgtgaagatg ctccttgtgc aaaggcatgt
    12541 ccaaatggtg gtatagttag ggttgggaac acaataaaaa taaatgaaga aaattgtata
    12601 ggatgtaaga cttgtatgtt agcatgtcct ataggtgcaa tagacatagt tacattaaaa
    12661 gatgtagatg aaggaaagct ttgctttaga gaaagaatga cagcaaataa gtgtgatttt
    12721 tgtatagaat cgccagaagg gcctgcatgt gtaaatgttt gtccaactaa ggcatttact
    12781 atagttaagg atgaggatat agatagtaaa gttaaaaata agagaaaatt agctatattg
    12841 taatataaaa aagatggagt tttatttatg attaaaagct ccatcttttt tattataata
    12901 tttatatatc taaaaatcaa ttattgcatt agtttatcta atctttcttt attcaatttt
    12961 ttcattccta tagaagttat aataagtaaa gtataggcaa ttataactga taaaattata
    13021 atagaccaag aaaatctgta tccaaaatta tcaactaaca atccaaataa tgttgaacct
    13081 aatgcaaatc ccactgcaaa aattaattgt acgattccat atatgctacc aaactccttt
    13141 tgaccaaaga aagagccagt taaataagca ggaccaatca tataagcaaa tacagaaaga
    13201 cctttcaaaa tggcaaatac aaatgcaaaa gtagaagatt tatctaccaa tagtaatgat
    13261 aaagttgcta taagaaccaa tacaaaagat ataataagtg gctttgtttg accgaattta
    13321 tcaaacaata atcctccaaa taaattacca atgagagcaa ataaagcaaa tgtggagcct
    13381 actgttccta ttagaatagg atttagataa tttctaagat aagttggata ttgtacggat
    13441 aatgatgaaa catatatacc aacaaatata aatccaatag tcataatcca aaaatattta
    13501 atttttttag cttcttttaa tgtataaccc catactgaag gagaaattgt atcagatgct
    13561 ttctcttttt tacctttaga ttttaccttt tcattaatat ctttaggcat cctgagtatg
    13621 aataatatta caggtatacc aactattaat gctataattc caaatattag ataagaacga
    13681 ctgtatcctt gagttgctaa taagcgaact gcaagtggtt gtagaaatat actaccaata
    13741 gaaccaccag caaaagcaaa gcccaaagct tttcctttgc tttcttcatc aaaccaagca
    13801 cttattaaaa caggtattcc tatagcagat ataattgtag cgccaatttg aacaatacat
    13861 gatacagtgt aaaattgcca tagttcagta catatagaaa aagtagcaaa agcaatacta
    13921 gaaattatac aacctaataa atatagtatc ttaatattga ctttagaaaa taatacacct
    13981 ataaaaggtg atgcaattgc tgaggcaata gtaccaatag tgaatattag agaaaaagaa
    14041 gctaaactaa agccttctcc ttctacaaca taacttataa attgaggctg tatacttgct
    14101 acaatactaa atggaacagc ttgtatcaac atacaaccaa gtactataac ccaagcattt
    14161 gaaaagtttt taatgctaaa tttattgttt agtaatgtat tcatggtatc tctcctttag
    14221 gttatgattt gtagatatta atctaattat tctaatttta tatattagtt ttggatagcc
    14281 caactattta aatataattt gggtattaat ataaaaatta actataaaaa ttgattattt
    14341 taaatgtatg gggtaaatta aagtatataa ataaatgaaa ggatggataa aatgtcattt
    14401 gaaaaaataa ataataataa cctagataaa atacacaaca ggaagtgtat tatgcttgta
    14461 aactttaata aaaaagaatt atcattaatt aaaaacgtaa gtaactttgc tggaataaga
    14521 gaccatatca tcttaagtga taaaaatgga gagaatataa taagagacat tttagatgga
    14581 aatatatcaa atgaatgtga aaatgggatt aagagtaagt ctgtaatatt taataacact
    14641 ccaagcacac aagtaaatgc atttttagaa gggctaaaaa aagtgagaat aaatagacct
    14701 tttacagcta tggtaacaga gacatctatt gattggacat tgaataattt aatagaaaat
    14761 ttagttcaag aacgtaatgc tatgtcttta ggaaaaacaa ttgaacataa gtaaatttaa
    14821 gatatcttat agtaatttta actaatgtac attgagtatc atattatatt ttagaatata
    14881 aaattttaaa tgtgggtaga tggaggagat ttgagatgaa agttttattt acaataaatt
    14941 atggaaaaga aaaatttgat aaaataagag aattaggcta tgatgtcata cattactctg
    15001 aagatagtat tgaaaataat gaggatgtaa atacagcaga tgtactagtt acatacaatc
    15061 catttgagag attagatatt tctaagatga aaaatttaca atatatacag acaactagtg
    15121 taggtataga ccaacttcca aaagaagagg tattgaagag aaatattaag atagctaata
    15181 atagaggcgg atatagtgta cctattggtg aaagtattgt attgtatatt ttagaaatat
    15241 ataaaaatag tgcaaaattt tttaaacaac aacaaaataa agaatggaag atggattttt
    15301 ctgtatcaga attgtatgga caaagagttg gatttttagg aacaggtact atatcttcag
    15361 aagcagcaaa aagattaaaa gcttttggtg tagaaatatg gggtgtcaat actgatggaa
    15421 gtagtaaaaa atactttgat aagtgttttg ctactgaaga tatgaatatt gtatttgaag
    15481 aatgtgatat agtagttgta gcaatgcctt ctacaaaaag tacagatgga atagtaaaca
    15541 aagatatgtt taaacttatg aaagatggtt ctgtatttat aaatgtaggt agaggaaata
    15601 cagttaatca gaaagattta gaagaatgtg tagagaaatt tagaggggta gcattagacg
    15661 tatttgaaag tgaaccttta aacaaggaca ataagttgtg ggagtgcgaa aatgtcatag
    15721 taactcctca taattcttgg gtgtcagata aaaacaaaga gagaacattt aatatggttt
    15781 ataataattt gaaacactat ataatggaga ataaacctgt agataatgtt gtagacatat
    15841 caagaggcta ttaaaaataa aaaattttga taaaaataaa aactcttatt agtcgtatta
    15901 aagatactaa taagagtttt ttttgtaata aatttaaagc ccaaaaagtg atatactaaa
    15961 aaatatataa ataggagata attaattata gggaaatttc aaaataattt aattttaaaa
    16021 atatgaagag gtgcaaaaag tgagcgtaaa agatgaaatt aataaactta tagagacaat
    16081 aaactaccat aatgaaaggt attataatca agatagccca gaaatatcag attatgaata
    16141 cgataagtta atgaaggagt taatttcttt agaagaagaa catccagaac taaagagaat
    16201 tgattctcca acaaataggg taggaggtaa gccattagat aagttcaatc aaattgtaca
    16261 taaaactcct atgttgagtt tatcaaatgc tttttcagag caagatttga gagattttga
    16321 taagagagtt caagaatatg tgggagaaaa tgttgaatat gttgttgaat ttaaaataga
    16381 tggtctttct gtaggtctaa cttataacaa tggagagttt gaaaaaggag caactagagg
    16441 agatggagtt gtaggagaag atatttctca aaacttaatg acagtaaaaa gcatacctct
    16501 taagataaat gataaaaaag aactaatagt tagaggagaa gtatatatat caaaagataa
    16561 ttttagaaaa gtaaatgaac aacaagaaga acaagaacag ccattatttg ctaatccaag
    16621 gaatcttgca gctggttcat tgagacaatt agacccaaaa ctaacagcaa agagaccatt
    16681 agatatattt gtatttaata tggaaaatat agaagaatac gatttagaaa gtcacagtga
    16741 atcactagag tacttagaaa aactaggatt ttcagtaagt cagagttata aagtttgtaa
    16801 aagtatagat gaagttattg agcatattga atactggaca gataacaggg gtaatttaga
    16861 ctacgaaata gatggtatgg taataaaagt aaacaatata aaacaaagag acgaaatggg
    16921 atatacagcc aaaagtccta gatgggcaat tgcatataaa ttcccagcag aaaagaagaa
    16981 aactaaaata gtagatataa tagtagaagt tgggagaact ggtacaataa caccttcagc
    17041 aattcttgaa ccagtcagac ttgcaggtac tactgtaagt agagctacac ttcacaatga
    17101 agattatata agagaaaaag atataaaaat aaatgataca gtattagttc aaaaagcagg
    17161 ggacataata cctcaagttt tagaagttat aaaagaagaa cgtactggag atgaaataga
    17221 ttttaaaatg ccagaaaagt gtccagtttg ttcagagcca acaataagat tagaaggaga
    17281 agctgctgta aaatgtatca atatgtcttg cccagcccaa ataagaaggg gaattataca
    17341 ctttgtgtca agggatgcaa tgaatataga tggattggga gaatctataa ttacactgct
    17401 attaaatgaa aaaattattc aagatgtatc tgatttatat tatataaaaa aggaagatgt
    17461 agtcgaccta gagagaatgg gcgaaaaatc tgctgacaat ttaataaatt caattgaaaa
    17521 atcaaaagaa aatgatttat ggagacttat aaatggatta ggtattaaat ttataggagt
    17581 taaagcagct aagatacttg catataactt taaggattta gatgaagtaa tttctgcaac
    17641 tagtgagcaa ctagaagaat tagaggaatt tggaagtata atggcaaaca gcgttgtcga
    17701 gttttttaag gaagacaaaa atatgaacgt tataaacaag ttaaaaaatg taggagtaaa
    17761 tacaaaatca ttggataata caggtgaaaa agtagaaaaa atatttgaag ggatgaaaat
    17821 cgtattgact ggtacacttc ctacattaaa aagaaatgat gcaaaagata tgatagaaaa
    17881 aagaggtgga aaagctacat ctagtgttag taaatcgaca acatttgttt tggcaggtga
    17941 agaagctggt tctaaactta ctaaagcaaa tgatttagga attaaagtta tagatgaaga
    18001 gagattttta gaattattaa agttatcatc gaaagatgaa gtaaaagctg tattagagag
    18061 ttaaaaagtc gcttaattta gtagaaaact actataatta atgatagaat agttcagtaa
    18121 gctaataaga ttacgaaaaa tgacaataat taatatttga caaaattaaa aattataaaa
    18181 ataaatttat aaaaaataaa aaatatgaga aaggatataa aaaatgacca gaagttatga
    18241 tagaagtaac gaacagataa gacctgtaaa gataactaga gattatacaa agtatgcaga
    18301 aggttctgtt cttatagaaa tgggagagac aaaggttata tgcactgcat cagtggaaga
    18361 aaaagttcct ccttttttaa gaaatagtgg tactggatgg ataaatgctg aatattctat
    18421 gctaccaaga gcaacacaac aaagaaagat aagagatgca tctaggggaa aaattgatgg
    18481 aagaacacaa gaaatacaga ggttaatagg aagagcaata aggtctgttg tagacttgaa
    18541 taaattagga gaaagaacta tttgggtaga ttgtgatgtg attcaggcag atggtggaac
    18601 tagaactgca tctataacag gggcatttgt agcagttgca gaagcaatat ataaattata
    18661 taaagcaaag ataataaaag agatgccaat aacaaatttt gtgtctgcaa ttagtgttgg
    18721 tattgtaaat ggaatacaca tgctagattt atgttatgaa gaagattcta aagcacaggt
    18781 agatatgaat ataataatga ctgataagtg tgagtttgta gaagtacagg ggacaggaga
    18841 agagaaacct ttttcgagag cagatttaaa tttattgtta gagttaggtg aaaaagggaa
    18901 taaagaatta ataaggcttc aaagagaagc tttaggtgaa atatcagatg agatattggg
    18961 tatggattat ggtaatgaag tagtgattgc tactaataat gctcataagc tagaagaaat
    19021 aggtgaaatc ttaaaagatt ttgaatataa agtgtactct ttaaaagatg ttgatttagc
    19081 aggtatagag atagtagaag atggaaaaac atttgagcat aatgcgttga taaaagctag
    19141 aacaatagca aaaaagacta aacttatagc aatatcagat gattctggct tagaagtaga
    19201 tgctttaggc aaaaaacctg gtgtttattc agctagatat gcaggagaac atgcaacaga
    19261 tgaagaaaat agaaaaaaac tgttaaaagc tatgcaaaat ataccaatga gcaaaagaaa
    19321 tgcaagattc gtctctgcaa tagcagtagt ttttcctgat ggaaaagaat ttgtagtaag
    19381 aggaatttgt gaaggaatga taggatttga agaaaaaggt aaaaatggtt ttggatatga
    19441 tagcttattt atagtaaaag gatatgataa gacttttggt gaaataccta gtgttataaa
    19501 aaattcaatc agtcatagag caaacgcact aaaacttatg aaacaagagt ttataaaaag
    19561 ggtagtgaag taatgaaaat aggcatagta agcgacacac atatgatgat taaaaatatg
    19621 gaaaaaacaa taccatattt gaaagaatgt gacctaataa ttcatgcagg agataacttt
    19681 actgattcaa gatatattca tagcatgact aatgtaggta ttattgcagt taaaggtaat
    19741 tgtgactttg atgctgttga ggaagaagtt gtctttgaag tggcaaataa aaccatattc
    19801 ttatgccatg gagataagta tggcgtaaaa tatggaactc atatgttaga aaaaaaggca
    19861 actgaagttg atgctgatat agttatattt ggccataccc ataccccatt tagagaaatt
    19921 aaagatggtg tactttacat aaatcctgga agcacttcac ttccaagagg agtatcgtat
    19981 aaaagttttg tcattatgga tatagaagaa gatgacataa aaattgaaga aatacgcata
    20041 taaaggtgta tttatctaaa aaaatggcaa aaaacgctca aaaaattaaa ttttatcaaa
    20101 taagtacaac gatattaatg gaacacttgt aaataaaaaa acttatgtga tataattaag
    20161 tggttgaatt ataaatagaa tgaattataa taccgttatc gggtattaca taaatatcac
    20221 taggaggatt agaaggatga aagcagaact aattaaaaaa gagggtaata aagttacttt
    20281 aaaaataaca gtagataaca ataaatttga agcagcagta aataaggctt ataataaaag
    20341 cagaaataaa tacaatatac ctggatttag aaaaggtaaa gctccaagaa ttgttataga
    20401 gactcaatac ggaaaaggta tattctacaa tgatgctata gaaatattat tcccagaagt
    20461 ttatccagaa gctatcaagg aattagacat agacccaata gacaatccag atttagatat
    20521 agaagaaata agtaaagaca atgggttggt tatggttctt aatgtagaag ttaaaccaga
    20581 gtttgaatta ggaaactaca agggtataga aatagctaag gttgagaata ctgtaagtga
    20641 cgaaaatgtt gatgctaaac tacaagaaat ggttgaaaaa aatgccagat tagtaagtgt
    20701 agaagacaaa gctttagaag atggagatac agctataata gacttcgaag gttttgaaaa
    20761 tggtgtagct tttgatggtg gtaaaggtga aaattataac ttagttatag gttcaaacac
    20821 atttatacca ggatttgaag aacaattagt aggtaaaaaa gctggtgaag aagtagaagt
    20881 taatgttact ttcccagaag aatatcactc acaagattta gctggaaaac cagttgtatt
    20941 taatgtaaaa ataaatgatg taaaagtaaa agaattatct gcacttgatg atgaatttgc
    21001 taaagatact agtgaatttg attctttaga tgaattaaaa gcagatgtaa gagctaagtt
    21061 agaagaagaa gctaaaaata gagcagatgc agaaactaga aattcagtag tagaaaaagt
    21121 tgctgaaaat acagaaatag aaataccaga agttatgata caacatcaaa tagataatat
    21181 gttaaatgaa cttaactacc aattacaata tcaaggattt ggattacaac aattactaga
    21241 gatgactggt agaactatgg aagaattaag agaagagaga aaagaagatg ctaaaaaatt
    21301 agttaagtct tctttagtat tagaagcaat aactaaagct gaaggaattg aagcaacaga
    21361 agaagaattt aaggctgagt tagaaaaaat ggcttctgct tacaatatgg aagtagaaaa
    21421 aatagaagct tctttaagag atgccgacaa agaagatata aaaggtcaaa taaaaataag
    21481 aaaaacaata gatttattag ttgataatgc aacaatagct taataaaata aacgctaact
    21541 aatatagttg acgttatgaa atgtttagta tataatgatg tgcaaatata atttagttaa
    21601 atgcgtataa taatttacag tagcttaaaa aaagctactg taaattatat agaataaagg
    21661 gggtatgaaa ccatggcatt agtacctgta gttgttgaac aaacaggaag aggagaaaga
    21721 tcttatgata ttttttctag actattaaaa gacagaataa tcttcttagg agatcaagtt
    21781 aacgatgcca cagcagggct tatagtagca cagctattat ttttagaagc tgaagaccca
    21841 gacaaagata tacatttata tataaattct ccaggaggaa gtataacttc tggaatggct
    21901 atatatgata ctatgcaata catcaaacca gatgtaagta ctatatgtat aggtatggct
    21961 gcttctatgg gagcattctt gttggcagca ggagcaaaag gaaaaagact agcactacca
    22021 aatagtgaaa taatgataca tcaaccatta ggtggagctc aaggtcaagc aacagatata
    22081 gaaattcatg caaagagaat tttaaaaata aaagaaactt taaatgagat attatctgaa
    22141 agaactggtc agccattaga aaaaattaaa atggatactg aacgtgataa cttcatgagt
    22201 gcactagaag ccaaagaata tggtttaata gatgaagtat ttacaaagag accgtagata
    22261 gagagaggtg ttaatatgtc taaatatgaa gaaaaacgac aattaaagtg ttcattctgc
    22321 ggtaaaaatc aagatcaagt aagaagatta attgcaggtc caaatgtata tatttgtgac
    22381 gagtgtgttg aactttgtga cgaaattatt caagaagaga tagaagatac tatagatgag
    22441 gatactacat ctttaccaaa accaaaggaa atgatggaga ttctaaatga ttatgtaata
    22501 ggtcaagaaa aagctaaaaa agctttatca gttgcggttt ataatcatta taagagaata
    22561 tacagtaaaa aatctagtag taaggatata gaaattcaaa agagtaatat acttttatta
    22621 ggacctacag gttctggaaa gacattactt gctcaaactc tagcaagaac tctaaatgta
    22681 ccttttgcaa tggcagatgc tacatcattg actgaagctg gatatgttgg agaagacgtt
    22741 gaaaatatac ttttaaaact tattcaagct gctgattttg atatagaaaa agctgaaaga
    22801 ggtataatat atatagatga gatagataag attgcaagaa aatctgaaaa cccatctata
    22861 actagagatg ttagtggtga aggtgttcaa caagcacttc taaaaatatt agaaggtact
    22921 gttgcaaatg ttccaccaca aggaggaaga aaacatcctc atcaagaatt tttaaagata
    22981 gatactacta atgtattatt tatattaggt ggagcttttg atggattaga gaagataatt
    23041 caaaaacgtg gtggagataa aacactaggt tttggagcta aaatagaaag taaaaaagaa
    23101 ttagatttag ggaagctata tgaaaaagta cttccagagg atttattaaa atatggtata
    23161 ataccagagt ttataggaag aataccagtt ttagctactt tagagttgtt ggatgaagat
    23221 gcattaatgc agatattaca ggaacctaaa aatgctctag taaaacagta taaaaagtta
    23281 ttggagttag atgatgttga gctagaattt gaagaaggtg ctcttcgtgc aatcgctaaa
    23341 aaggccatag aaagaaatac aggagctaga ggacttagaa gtatagttga aagcgtaatg
    23401 atggaaacta tgtttgaagt accatctaga gataatataa agaaggttat agtaacagag
    23461 aaatctgtga acgaagactc agtaaatcct atcatagtac ttaaggatca agaggaaagt
    23521 gcataagatg atgaaaagga gctttaaaag ctcctttttt tatgttaata tgctaaatat
    23581 aaaataggta tcattattat aaaaatgtga gtaatatacc tagaatacca aagacgaata
    23641 caattgcaat ggaaccaatt tttcctttat ataaaagata aaatgatgct ataaatgcaa
    23701 ttattccata taaatcccaa ttaacttctt tggcagatga aaaagctgct gataatatga
    23761 atcctagagt aattggtcta agaactgtca ataagttttc taccttttta ttatttttaa
    23821 atttagaaaa taactttgat ataatagtta gacctattaa tgcaaaaatt gtaacaccaa
    23881 tagttgcggc tagagaacca aagaagccag cagtcttaaa tcctacaaat gtagcactat
    23941 ttattgctac aggacctggt gtagattgtg atatagcaag taaatccaaa aactcttgat
    24001 tagttataaa gtgatattta gatataaatt cttgctggat aaatggtagc atggcataac
    24061 cgccaccaaa actaaatgcc cctattttaa ggaatattaa gaagagtagt attatttctg
    24121 acatagacta tatacacctc ctccaattat taaataaata gggcttatat tgaatacacc
    24181 aacagataaa actgctataa ttaatagtag tatgttagtt ttagactttg gtagtttctt
    24241 gaacatgctc gtaaaagaat acaatataag agctattaca acaggcgcaa ctcctttaaa
    24301 aaatccatct aaaattttag atgatgaatt tttaaagtat agatacgata agactaagac
    24361 tatacaaaat gatggtaaag cagagcctaa ggtacatata agaactcctg gtataccata
    24421 aagtttatat cctaataata cagatatatt aactgccagt actccaggat aactttgagc
    24481 tatggataaa taatcaataa attcttcctg ctctaaaagt ttttgtttgt ttacaagttc
    24541 atcttgtata agaggaacca tagcgtatcc accaccaaat gtaaacgcac caatttttaa
    24601 aaaggttaaa aacaatttca acataataga aacctccaaa agataaataa catctaagta
    24661 tactaatatt aatattatat cataataaaa agataatata gtatgatagt attctaagaa
    24721 tttatcatta atgttgaaaa ttaacttgtt caaatatata atattaaaag caaagcaatt
    24781 agactagaaa aggagtaatg ttgatggaac aaaattatac taaaatagat catgaattac
    24841 cattaattcc tctaagagga ttagctatat ttccatatat gatattaaat tttgatatag
    24901 gtagagagat atctcttaaa gctttagacc aagctatgat ggatgaagaa cttatatttt
    24961 taacttctca gaaagaagca gaggtagatg agccaggaga agaggatttt tatcatgtgg
    25021 gaacaatctg taaagttaag caaatgataa aattaccagg agatacagtt cgtgttttgg
    25081 ttgaaggtgt atcaaggggt agagtaaaga aaatagaaca agaagatggg tatttcagag
    25141 cagtaataga agaaatagta tttgatagtg ataatttgga tagtgaaaca gaagttgaaa
    25201 ttgaagcatt tgtaagaaat gtatttgatg cctttgaaga gtatataaat attggaaata
    25261 gggtatcacc agaaatatta atatctttag ctgatataga agatgtagat agatttatag
    25321 atacgatagc tgcaaacata tatctaaaat caagccaaaa acaagaaata ttagaagaat
    25381 ttgatattag aaaaagactt gaacttattt attctatatt attagaggaa atagatatat
    25441 taaaaataga aaagaaaata actttaagag taaaaaaaca gatgaataag gttcaaaaag
    25501 aatattatct tagagaacaa ttaaaagcta tacagaaaga attaggtgaa gaagaagata
    25561 taaactctga agcagatgag tatagagaaa agcttaaaaa aataaaagca cctaagacaa
    25621 ctaaagagaa aatagaaaaa gagatagata aattttcaaa aatatcttct atgtctccag
    25681 atgtatctgt aagtagaaac tatttagata ctatattctc attgccatgg aataaagaga
    25741 caaaagataa attagatata actaaggcaa aagatatatt agatgaagac cattatggat
    25801 tagaaaaagt taaggagaga atactagagt atctagctat aagaactctt gctaagtcac
    25861 ttaaagggcc tataatatgt ctagttggtc ctccaggaac aggaaagaca tctatagtaa
    25921 aatcaatcgc cagagcatta aatagaaaat ttgtaagaat atctttgggt ggagttagag
    25981 atgaagcaga aataagaggt catagaagaa cttatgtagg ttcaattcca ggaagaataa
    26041 ttaatggggt aaaagaagcc caaacaaaaa atcctgtatt cttatttgat gagatagata
    26101 agatggcagc agattataaa ggtgacccag cttcagcaat gctagaagta ttagacccag
    26161 agcaaaataa ggattttgtg gaccattact tagaaatacc ttttgattta tcaaaaatac
    26221 tatttgttac aacagcaaat agtctaagta atataccaag accattatta gatagaatgg
    26281 aagtaataga agtatctgga tatatagaag aagaaaaatt aaatatagca aaaaaatacc
    26341 tacttccaaa acaaattaaa gaacatgctt taaaagaaaa ctttataaaa atagatgatg
    26401 aaactttaag aagtataatc aaccattata caagagaagc tggtgtaaga acactagaaa
    26461 gaactattgg taagatttgt agaaaggttg ccaaaaaata cgttgaagac ccaactcttg
    26521 aagaggttgt tataaataag tctgacctag agacatatct tggaaaagac atgtttaagt
    26581 atcaacttgc agaagttaac cctcaaatag gattagtaaa tggtcttgcg tggacagaag
    26641 ttggtggagt tacacttgaa gtagaagtaa acgtacttaa aggtaaaggc gaaattgtat
    26701 taactggaaa attaggagat gttatgaagg aatctgcgaa gacaggtatc tcttatataa
    26761 ggtcaatagt ggacaagttt gatatagacc ctgaatttta taagacgaat gatattcata
    26821 tacacattcc agaaggtgct gttccaaaag atggaccatc agctggtata actatggctc
    26881 ttgcagtaat ctcagcactt actaaaagac ctgtgcctgg aaatatagca atgacaggag
    26941 agataacttt aagaggtaga gtcttagcag taggaggagt taaagaaaag ttattagcag
    27001 ctcatagagc tggtataaca aaagtattaa ttcctaaaga atgtgaagct gatttagatg
    27061 aaataccaga aaatgtaaaa gaaaaaatgg agtttgtgct tgtagaacat atggatgaag
    27121 tattagaaca agctttatta aagagtggtg aaaataatga aaattagaag ttcagagata
    27181 actatgagtg ctgtgaataa atcacagtat ccagcagaag gaatcccaga gatagcattg
    27241 gctggtaggt caaatgttgg aaaatcatca ataataaata cattgcttaa cagaagaaac
    27301 tttgctagaa ccagtcaaac accaggaaaa actagaacta taaattttta tctaataaat
    27361 aatgaatttt attttgtaga ccttccaggc tatggatatg caaagatagc taagtctgaa
    27421 aaagaaaagt ggggaggtat aatggaacga tatctagaaa gtagacaaga gttatgttct
    27481 atattcctac ttgtagatat tagacatgag cctacagcag atgataagct tatgtatgaa
    27541 tggataaagc actttggata taactgtgta gttattgcaa ctaaggcaga taaaatatct
    27601 agaggacaat atcaaaaaca cataagtata ataagaaaaa aattgcaaat ggaaagtagt
    27661 gaaaaagtaa ttcctgtttc ttcactaaaa aagactggtg ttgaagaatt atgggaagag
    27721 attgtaaatc aatacaatca acatggatat gaaataacag tagattaaat ttaaaatgtt
    27781 aaatattgac ataagattta tttgaataat aaatgtaata atagttagtt ttaattatat
    27841 taatattaaa gtaaaatata ggatgcctta aaatagtttt aatgaacttt tttttggcat
    27901 ccttattgtt tataaacaat aaatatcagt aattttgcta aatactaata aattttagta
    27961 aggtatggga aaattagtat aaaatagtta aactaaaaaa ttattgattt atgtagtcag
    28021 taataaatag catttaagaa atagatttat ctgttttcct cataaagaaa acagttagta
    28081 taagccctac aaaacatact gatgctgcaa tccaataaga ctgattgcta gctataacat
    28141 aggctgatgt gtgactttta aacttttgat tgtatgtaag actttgtgca aaaaaactac
    28201 taaaaaatgc tgttccaaga gcaaatgata aattacgaat agttgcaaga aaactgcctc
    28261 cgtacccctg tttttgagga ggaacatctg ctaaaatact actattgttt ggtgaactaa
    28321 atgctcctag cccaagtcca aaaataacta aacatacaat taaaaggtac aatggagaat
    28381 ctgcttttaa aagtgccatt ccaactagtg agatggtcat aaatgaaaaa gatgctgtta
    28441 gaatgttttt tgcaccgtgt ttatcagaaa gtgaacctgc aataggtgct ataagtacca
    28501 taataagtgg atgtacagtc attacataac cagccatcat aggagataaa ttttttaatt
    28561 gttctaaata aaatggaagt agaaaagata ctgccatttg aggaaaataa caagctactc
    28621 caatgatatt gcctacagta aagtttctat ttttaaatag agggaggtca ataaagcttt
    28681 gttcaaattt tacttctctt aatgccaaca ataagaagat tataagtgct gttataccta
    28741 aatacaatct tgtattaccc ttctcattca ttgcaagtaa tataagcgta gtgcaagatg
    28801 cgaaaagtag aagacctgga atatcaaagc tcttgttttg ttcatccaaa acaggtttag
    28861 gcagaaattt aatgccaaga aagactgcta taataccaat agggatattg ataataaaaa
    28921 tagaaggcca tccaaaatgt tctaaaatta tacctccaat tacaggacca ctcatatttc
    28981 ctataccaac tacagctcca gtgattccta tagcttttcc tttttcattt gctggaaatg
    29041 cattggaaac tataccaagc ccagtagcca ttaaaatact tgctccaact gcctgaaaga
    29101 ttcttgaaga aattaatgtt gataaattgt ttgacatact acacagaaaa gaacctattg
    29161 taaatataaa aaatcctaat gtgtatagac gatttgaacc tactttatcg ccaagttttc
    29221 cgaaaaataa catagtagct gttacaacaa gcatataagc tgttacaacc cactgaattt
    29281 gtgacatgtt aattttaaag tcagcagcaa ttgtaggatt tgcgatattt agtatacttc
    29341 catcaagagt agacataaat acaccaatac agatgataat caatactatc catttttgtt
    29401 tcatgataac actccaatct taatagattt tataagtacg atataattgt aaaataaaca
    29461 acaaaaaaag cacctgtata ttaggtacct tgaatgtgtc taatttcatg aataaatacg
    29521 gtagttgaag gatttgtata gattactcat gtaaaattac ataaaggtac tcatatacta
    29581 ttttcttcta ccatccagat tttactgtcg gctttggaat atgaccaaat tatgccataa
    29641 ggctcgcggg cttttaccgc cgataggtaa tttcacccgt ttatgaagaa aaacagatgt
    29701 attttgtaat attattttaa cataaaatta taatggaaac aattggattt aagtaatatc
    29761 cagctgtatt acaatacaaa tatttcaata tggacactta aattgtattt atagatagaa
    29821 taaggcacag tatgagaatc tataataatt agataaaatt aacctgttat tagtattaga
    29881 tggcaagttt taaatttgtc aaaatcagaa agctgtaata aaattcttat aatatgaata
    29941 tcttaattaa gagatattgt aaattatgag gaggcgctaa tatggaagga aaggtaaaga
    30001 aggcattccc gggtggaaat acatcacaag gctcatattc tttttttgac tatataattc
    30061 ctgaaaatgt aaaaagagtt ttttgtttaa agggtggacc tggagtagga aaatcctcac
    30121 ttatgaaaaa aatttataaa gagtttttgc atagaggata tgatattgat ttatatcatt
    30181 gtccttctga cccaagttcg ttggatggtt tagttataaa gaaattaggc gtggtactta
    30241 tggatgcaac tgccccacat actatggacc caaagctacc tggagctttg gatgagataa
    30301 ttaatttagg tgattactgg aatacagaag aattagaaaa gaataaacaa gaaatatcag
    30361 tttatgataa agatattagt aattcattta aaagagcatt taagttttta aaatcagcag
    30421 aaccaatctt tagagacata gaagaaaaat attctgattg tatggattat ggcaaagtaa
    30481 atttggtcac agaagaattt ataaaaaggt tatttgatgg agttaaaagc acaggaaatc
    30541 taaaaagaga aaagcattta tttggttcag ctataactcc tgtagggtgt ttagattata
    30601 cagaaaacat tttaaaagat gtaaagaaag tctattattt agatggagaa ataggaactg
    30661 ggaaaactac attattaaac aaagtatata agaaagctat tgaaaaaggt ttaaatgtag
    30721 aagtttatca ttatccactt attccagaga aaattgaaac tgtactatta actgatttag
    30781 atataggcat aactataagt acgactttta aagaagaaga tacaattgat ttaaatcagt
    30841 ttttaaatag agaaaaacta cttaaatatg aagaagatat taaatttgat gaaaaagttt
    30901 tagatgattt aatttgttgg gcagtactaa atcttaagag agccaagtct aaacatgata
    30961 ttatagaaag ttactatagt cctaatatga gatttgatga agtagggaaa ttaagagata
    31021 aattaattaa aagaatttta aaatatgagg aaaacttata aacgtatatg tttaaaatta
    31081 atgttaataa tgatataatt tttagtaaat tggtgtagct ttgaggctat gccattttac
    31141 ttttttttgc taaaaaatga tattaataat ctccaaagca attatttgtt tttcataatt
    31201 attatttcct ttcctaattt atgttttttt ataatatcaa aacaatgttt attgaaagtt
    31261 tatataattt taagaacatc taaaaatgca tacttgatat tggtaaatta gtgataggag
    31321 ttcaatgtat gatattatat actaaagatg aattttaata tataactttg atataataag
    31381 catatgtaaa tgtttaatat ttaattaaag aaaattatca tataaaaata aatttgtaag
    31441 gagcaataca gtatgaaaaa acaatatgtg tttataatac tagcaagtac tattttaggt
    31501 atacttgttg gaaccatatt tagagattat agcaatgaag aaacagttta tacaagcaaa
    31561 gacaagtcaa taagaaaaga aataaaaatg acaagaaaat ctataaaaga attaaccaaa
    31621 gagaaagaca agttagataa agaaattaat tcaatgaaac aaaatcaaaa aaattctgaa
    31681 gaaatatcag aaatagaaac acttagagat agtctatcat attcagatgt aaagggtagt
    31741 gggataatat taagtataga tgctatagat gaaaaaacag ggaatatagc taatagtata
    31801 gactataata agattcttgt aaaagttata aatgaattaa aagtaaacgg aggaaaatac
    31861 atagaaataa atggtcaaag aattaatcaa tattcagaag ttgtacttgc aggaagccat
    31921 ataaatataa atggagttcc tgtagcacaa ccatatgaaa taaagtgcat tggagataag
    31981 gagaaactat ctaattatgt agataaaggt agtaaatatt tagatagcat acaaaccaat
    32041 tatccaatga aaataagcat aaaaactgaa gaaaatatct ctatgaaaaa aatgaatata
    32101 cctaataaat taaagtatat aaagggtgat taagattata ttagaggaaa gcagtttgcc
    32161 aatgttaata ccagaaaaac ttgcaaaaaa atataatgta tttccagttg agtttcaaga
    32221 tgataattta gttgtagaaa tagaagatga ggatatttat gctctacagg acttgagatt
    32281 agctacaggt aaagaaatta tattgaagaa ggagaaaaaa gaacttataa gtgaaaagat
    32341 tgaaaaatat tataatagct ttgaacttga tgaggattat gcaaaaaaat tatttgaaaa
    32401 tcttttagaa aaagctataa aagaaaacgc aagtgatata catatagaac catttaaaga
    32461 acatttaata gtgagaatga gggtagatgg agaactaaag gaagtatcaa agtttttagt
    32521 tgatgtctat ccatcattgt ctacggttat aaaattaaag gcatcaatgg atataacaga
    32581 aaaaaggctt cctcaggatg gtagggtgga tataaagtta ggtggaaatt taattgacat
    32641 tagagtttca tcaataccaa ctgtctatgg tgaaaaaata gttttgagga tacttaatag
    32701 agatactttt ttaaaagaca aattagaact tggtttttca gaggaagcta taaaatctat
    32761 aaatcgtata ataaataaaa gagcaggaat attattagtg acaggtccta caggtagtgg
    32821 aaagactaca acagtatatt ccttattaaa tgatttaaaa ggaataaata aaaatattat
    32881 gactatagaa aatcctgtag agtataagat ggatggcata agtcagattc aagtaaattc
    32941 aaaagtaggg ttgagctttg atgtaggtct tagagcgata ttaagacaag acccagatat
    33001 aataatggtc ggagaaataa gagatgcaga aacagctaag atagcagtaa gagcagctat
    33061 aacaggtcat ttagttataa gtactttaca tactaatgat gcagtatctt ccatagctag
    33121 attactagaa atgcaaatac caccatatct tttaaatgct tctcttatag gtgttatatc
    33181 tcaaaagttg gttagaaaag tctgcaatca ttgtagccat gagattatga taaaagataa
    33241 ttttagtgga gatgtaaata ctaaggtagc tgtagggtgt gaaaaatgta atgataaagg
    33301 atactttgga agaacagcaa tttatgaaat cttagaaatt aatgatgata taaagacatg
    33361 tataagaaat atggaggatt ctagtactat taaagatgta gcacaaaata atggaatgat
    33421 aacatttgaa gatagttgta aaagactaat aaatgaaaaa ataacgacat tagaagagtg
    33481 tttagtagta aatgaaatga tttaactgaa ttgtatagtt aaacttcgtt tggtcagttg
    33541 tctttattta atctatttta attaagatgt ttgagtataa attatattta agtgaaataa
    33601 aattaaatgc attaaattag aaaatattaa gtttatttaa ttgtaattaa tataatttgg
    33661 aaggtattat agtacatgag attatctaaa aatatatttt ataatttaga aaagttttat
    33721 ttagtattaa atagaatcag tgataaagaa attagagtaa tttgcaaaga gataggcata
    33781 ttgctggaat ctggatgtga aattacaaaa atatttgaaa tcattgagag tcagtcaagt
    33841 aaaaaagcaa agaatttgct tagtatagta tcaaatcata ttcaaaaagg taactcaata
    33901 gcagaatcat ttcaaataac aggtatattt tctaaattct ttataagtat gataaaagca
    33961 ggagaaacta gtggaaactt agatataata atgagtgatt tatctaatta ttatgataaa
    34021 gaatacaaat taaaaatgaa aataataact atttcaatat atccaataat acttataata
    34081 ttatctatct tatcaatgtt gtttatattc gtatttgtaa tacctaattt tcaggtggtt
    34141 tttaccaaca atggaattga accaccttta ataactagag ttttaatggg aatatctaca
    34201 gtggttacaa ataacttagc ttatataata tttagtttta tattgttttc aataggaagc
    34261 atttatttct ttataacaaa tgataatata aaaaaattga ttaattcgtt aaaattcaaa
    34321 atacctttta taaaaaaaat taatcagtta gtagctacaa caagattttc aagggcattg
    34381 tccgttttaa ttagcagtgg aatacaaatt gtggaagcta tagacatgtc agctagtgtt
    34441 atagacaacg aatttattta tgaaaggtta aagatttcca ataattatat aaaaaaagga
    34501 aatagcatag gtaaatcttt gaatttagca aatgtttttc caaaattatt tatttcaatg
    34561 gcaaatacag gagaagaaag tgggaaacta gataaaagtc taaatgtaat aaataaattt
    34621 tatgaaaatg aattaaataa caaaatagag caaataatga aatttataga acctatgcta
    34681 attgtattta tgggattaat tattggtact tttattttag caatggttgt tcctatgttt
    34741 gatgcaatta ctaattttta gagagaaaaa taaattttat tagatgaaac ttttgaaaag
    34801 gagagaatat ttaattgata aatttgataa ataaaaaacg aaaaggattt acacttgttg
    34861 aaatgattgt agtagtaact attttaggcg ttatatctag tatagcatta gttaagtata
    34921 gtaaggttca agaaagtgcc aaattaaatg cagactatac gaatgctgct aatatagtaa
    34981 ccgcagctag catggcaatt aatgatgatg aaaagacaat agactctcta agtgtagaga
    35041 cattgaagga aaagggatac ctaaatactg ttccagttcc tcagagtaca tcaggtaaat
    35101 tcgaacttgt cataaatgat agcggaacag atataagcgt aaatataaat tctaagcaat
    35161 tttatccaaa ataatatttt atattgattg ctgtaagttt taaaattatt tttatttaag
    35221 aaggagatgg ctataattaa aaaaattcta aacttagaaa taaatgatga ttatataagg
    35281 cttgtatttt taagaaaaag tcataaaaaa gtttttatag ataaatcaat tataaaagag
    35341 attaactttg atataaaaaa tgatagtaat tcgattgaga agattaaata tatagaagat
    35401 gtagttagat ttgtaaaaga ggagattggt aaatcaaaaa tattatataa aaatttatac
    35461 tttaacattc aaattcagga tatagtcatt agaaatgttg aagttctaaa tactaagaaa
    35521 aaaagagaca tattaagcat gattgaattt gaaataacac aatacattcc aataaacata
    35581 aataactatt caataaaata taaagttata aattctaaaa atgataaatt gaatatacaa
    35641 gtaattcttt tcccaaagag tattattcaa ttaattaaga acatttcaga aaatctaaat
    35701 atgaatccaa agactataaa tataaatttt gatatattac aaaagcttat aaacttaaat
    35761 ttaatagaga atttttcaga agaaggggta tttatagaat gtaaaaaaag tcagttaatt
    35821 ataaatataa caaaagataa aaaaataaga gaaacatact tgctatcaaa agagggaaaa
    35881 tcatatgagt tcataaaaag attattaagg gattttaagt atgtttatta ctatggtata
    35941 gaaaattgtt ttattgaaga atactttaaa tatacagata aatctattga agtaaatcca
    36001 ttaagattaa aagataaagt aaaagtagaa aataattttg atgcagattt agaagacaat
    36061 aggataaatt atataagtaa tataggaatg attatttaag tgaaagtgga taaaacgata
    36121 aattttctta atggatttaa aaaaactaaa ccatgtaata aaatctataa aataataata
    36181 gtcttaattt taacgtgtat tttatttaaa tcatttatgt atgtgaaatc tataaatgaa
    36241 cttgaagatt atataagtca aaataagcta gtggataaat caagacacac tttaccaaca
    36301 acagataaaa ctagagagaa attttctaaa ataaaactta atgaaataaa aaaaatatat
    36361 gaaacaattg gaataaatat tattactaaa atatcgatag ataaaaataa gctagagatt
    36421 gaaggtgtgt gtagtgattt agatgtttta aacaatctaa ggaaaataaa gtacataaaa
    36481 tattattaca tagacagtat tgtaagagat ggagaatatt atagatttag aataaaaagt
    36541 tagatgggtg attatttttg agattaaata atagagaaaa aaatctactt aaaatattgt
    36601 taatattagt tattatattg gcaagtgata tagcagtaag ttatttggaa gaaaacttaa
    36661 ctagtttgaa gaatgaaaag caaaatataa taaataaaat aaataatcaa gataattatc
    36721 aaataaagga taataagcat atagatgata aatatataga aagtgttgaa tcaaataaaa
    36781 atgaagaatt aaaaaataat tttaataact catttttaga taaaaaaaca aataaagata
    36841 tagtgttatt agtagaaaat gaactgagag atattgtgaa tgtagaatat attaatcaag
    36901 aaagtagttt agataatcac aataaaaaac aagttaattt agaaataaaa gttaatggag
    36961 aacttgcaaa tttgttgaaa cttgaaggtg taatgaagaa gttaagacta aataataaac
    37021 ttgaaaagat agaaattaat aaattaaaag tcaataattt tgatgttcaa aatattccaa
    37081 atgaatataa acaagaaaaa gacaatattg ttgaatgtaa aatgacagtt agagtcatat
    37141 aacaaaaaag aatagataaa gtaaaaaact attatcaatt aataaaattt tatagaggaa
    37201 ttagttttac gataattcat ttctacaaga gggattgttg atgattttat cgaaatccta
    37261 attaaagtta atatattgta ccaaaatata agtgaattta atagaatatt tacttatact
    37321 atagattgat taataaaaaa atggtataat aacaaattgt actttaattt aataataagg
    37381 gagtggaagt catattgtta gggagaaatg agctatgccc ttgtggaagt ggcaaaaaat
    37441 acaagaaatg ttgtttaaat aaagatattg tttcagaaag agcatgcaga aaaactgaac
    37501 tttcacaaaa acaatataca gatttatatt caaagttata tgattattca agacaggata
    37561 agtttaaaga agaatgtgaa aaagctaaag aaatgtttta tataatgcaa aatgatagtg
    37621 taaatgaaaa atttgaaaga ttttttaata cttatttaat acaagaccac ataatggaaa
    37681 ataaaaaggt tatgacagtt ggttttcttg aagaaaatgg tgcaaaccta agtcaatcag
    37741 aagtatcaat attaaaaagt atgtttgaat catacgtaag tatttatgag gtaaaagaag
    37801 tttctgctgg aaaaatagta ttaaaggact gtttaagtgg aaatgaactt tgtacagaag
    37861 atgtaaaact tctgagaagt tttaaaatcg gaagttgtat gatagctaga atagttgata
    37921 ttgaaggaac taacatattg atagatataa ctataagtat atcaaatgag gttaaagata
    37981 taattttaaa ggatattatg aatctattca atcaatatca agatgtatac aaagatatga
    38041 agtcgttctt aatttatcat acacatatac tatacaagta tatacaacag cttttagacc
    38101 caagtatagc tgaatattta aagagagaaa gagataaaaa cgatacaaaa gttaattcag
    38161 aaaatgaatt agctgaagaa gaatgtaaag ttttagatac tcttaaagaa aatgtagaaa
    38221 aagaaaatat agatgcatgt atagagtttt ggaatgaata taagaaaaat catgaaaata
    38281 taaaaggagc tgaaaatggt tgggcagctg ctgttgaata ctatgttaaa aaagaagcag
    38341 gacaacctat aactcaagtt caaatatcaa agaaatacga tataagctct agtacactag
    38401 gaaagagata caaggattta aaaatatctt aattaattat aaaaagcttt aagtcataga
    38461 taaagaatct atagtataat atatgtataa tgtttagaaa gaggtattaa taatgagtct
    38521 tagtttagga aatgcaattg ctgtatcaac gaactttata aaatctaacc cagctgtacc
    38581 tgtgtacaat ggagaagaaa tatatgcagt tatttatcat atagaaatag gatataataa
    38641 tggtgtatca cctgatacca aacaatatat aataatagac tataaagagt ttgatttatc
    38701 tatggataaa aaaaatgaac taataaatat tgctaaagag tactgtaaat ctaaaggtat
    38761 tgtagaaaac gaaaaccttg aaataaacct tttagacaat gaagaagcag agaacttctt
    38821 agaaaaggtg tttaataata tgaaggttat aagaggtctt ataactaaaa aaggataaat
    38881 aaatggacta tataggaata gaaaatataa caccttatga aaatacatat gaatttagtg
    38941 tatatgaata tgatgatgaa atcaccttag gtagtgaaaa gttatatgta tgtgaattaa
    39001 gggttgtatt gattaaagtt aattctctgt atgttgaaag attgcataaa tcagttgaag
    39061 caatggtctt agtaaaaaac ttgaaaaaag atttagataa aacacttgtt gtaaacaaaa
    39121 taaagaattt tgtgctagat gagatttggg tagaaaatct agtaaaagag aatatagaag
    39181 ttatatttgt agaaagctag gaataaccct agctttttta tattacaatc taagagagga
    39241 aaacaatatg ttaagagtat ctaatgtaaa acttggaatt gataaagata tatctttatt
    39301 aaaagatgtt atacttaaga agctaaaaat aaaagaaaaa aacttagtca aatatagtat
    39361 atataaagaa tctatagatg ctagaaaaaa aggaaaaatg gaatttgttt attctgtaga
    39421 tgtagaagtt aaagatgaaa ataaaatact taactcaaaa ataaaagatg taacaaaaat
    39481 aaaggaaatc agatacgtaa atgtacctgt gggtgataaa aaattaaaga ataatccttt
    39541 ggtaattggt agtggaccag ctggtttgtt tgcaggtctg cttttagctc aaatgggtta
    39601 tgaaccaatc atacttgaaa gaggtcttga tgtagacagt agaaccaaag atataagtga
    39661 tttttggaca actagaaaat taaaaaataa ttcaaacgtt caatttggag aaggtggagc
    39721 aggaactttt tcggatggta aacttacaac tagaataaaa gatatacgat gtagaaaagt
    39781 attagaagag ttagttcagt ttggggcacc agatgaaata ttatactcac ataagccaca
    39841 tgttggaaca gatatattaa aaaatgtagt taaaaatata agaaatgaaa taataagttt
    39901 aggtggtcaa gttagattta attcaaaagt aacagatata gttgtatcaa atgattctat
    39961 acaatctgta ataataaatg atactgaaaa aatagataca gatacaatta tattagctgt
    40021 tggacatagt gcaagagata cttatgaaat gttgtattca agaggtataa aaatagttca
    40081 aaaacctttt gcaataggag ctagagttga acatccacaa gatttaataa ataaagcaca
    40141 gtacaaggat ttttataatc accctaggtt aggagctgca gattatagac ttatagagca
    40201 tgtttcaaat ggaagaacag catatacttt ttgtatgtgt ccaggaggta gtgtcatagc
    40261 atctgcatca aatgattttg aaatagttac aaatggtatg agtgaacatg caagagataa
    40321 aataaatgca aatagtgcat ttttagtaaa tgtaacgcca gaagatttta agagtgataa
    40381 tccactagca ggtgttcatt tccagcagaa gtatgaaaaa ttagcttttg aattaggtgg
    40441 aaaaaattat aatgcaccag ttcagcttgt tggagatttt ataaacaata agacttcttt
    40501 gaatttagga agtgttgagc catcatatag accaggatat acacttacaa atttaagtga
    40561 atgtttgcct agcttcgtaa cagagactat gagagaagcg tttatatctc ttgataaaaa
    40621 attgaatggg tttgcaatgt atgatgctgt tttgacggga gtagaaacta ggtcatcatc
    40681 accaataaga atagtgagag atgaaaatac tttagaatca gtgagtatta agaatctata
    40741 tccatgtggt gaaggtgctg gatatgcagg tggtatagtt acagcagcag ttgatggtat
    40801 aaaatgtgca gaaaaaataa tacaaaaata caccaataat acaaaaattt aacataaact
    40861 taatgatttt ttaatattta acatgaattt aacattatca ctcctataat ttgataaaat
    40921 acaataggtt ataaaatttt tagatacagt aatctcttta caaggaggaa ataatttgaa
    40981 cattataata agtctaatgg gtggattagg tctgttctta tatggtatga accttatggg
    41041 agaaggtctt caaaaatctg ctgggacaaa gctaaaaaag ataataaaac tacttactag
    41101 taacctattt atgggagttt tggtaggtac aggagttaca gcagtaatac aaagttcaag
    41161 tgctactact gtaatggttg ttggtttcgt aaatgcagga ataatgactt taaaacaggc
    41221 aataggagtt ataatgggtg ctaatatagg gactactgtt acagcccaat tggtatcttt
    41281 cgacttaact ggtatggccc cagtagcatt aggtgtaggt attatacttt atttatttgg
    41341 aaataagcct agaataaaaa atatagccga aatacttata ggttttggta tattatttac
    41401 aggtatggat tttatgaaga tggcagtgga gccactaaga gactatcaag gatttactga
    41461 cttacttgtt acatttggta gatatccact acttggatta ttattgggat ttgggattac
    41521 agcaataata caaagttcaa gtgcatctat gggtatgttg gttgcactgg cagcagaagg
    41581 tctagtgcca ctatcagcag cactgccaat tctttatggc caaaatattg gtacatgtgt
    41641 aacttcactg ttatcaagta ttggtgctaa caaaaatgca cgaagagcag ctatgatgca
    41701 tctaatcttc aatgttttag gaacggtgat attcttaata ttcttgaata aaccagtagt
    41761 aagcatggtt acaagttggg atccaagcaa tgttgcaaga caaatagcca atacacatac
    41821 attgtttaat ataatatctg tattgatact tttaccattt acaagtctta ttataaaact
    41881 agctataaag cttgttcctg atagagcagg tgatatagat gaagatgaaa ctaaaactat
    41941 taaatatata gatgatagaa tgatagaaac tccttctata gctcttgcaa atacgataaa
    42001 agaagcactt cgtatgggtg agaaggcgaa ggaaagccta aatgcatcta tggaagcttt
    42061 agtagaacat tcaactgaaa aaatagataa gacatataga cgagagagat taatcaatga
    42121 tttacagaaa gctattttaa attatttact taaactttca aaagcagctt tagatgatga
    42181 ttcaagagaa gttgtggata ctttgtttaa tacagtcaat gatatagaaa gaattggtga
    42241 ccacgctaaa aatatagctg aattatcaca agttgcaata gattcaaata tttatttctc
    42301 agaagaagga caatctgaac ttgatgttat gtataataga gttgtttcag catatactta
    42361 tgcattagaa tcaatgagga gcgataatgt tgatttagct tgcaaggtta taaaaattga
    42421 agaacaagtt gatataatgg agaaatcttg tagagctaat cacatgtata gattgaataa
    42481 taatctatgt agtatagaaa atggagttat ttatttagat gtaataagta atttagagag
    42541 aatatctgac catgctgtca atatagcaca acaagttata gcgaagagat taggaaatga
    42601 ttaaaaataa ttaatttaaa ataatataaa atttaaaata gaggataaat aaaagagaaa
    42661 ctttaaatga tacgtttaga gtttcttttt ttgtaaattt atttgactaa aatttttaaa
    42721 tttatacata gttatagtat ttagaagaat cgatttcctg ataataaatg atgagatatg
    42781 tatgattttg tggcaaactt ttaaaaaaaa cacctatatt ttgtgttata atctctttaa
    42841 aacgaatatg ctgaaaagag ggatttaaat gggaaaaata aattttgatt atagtaaagc
    42901 tacagagttt ttttgtcaaa acgaaataga tgtcatgcaa ccatatgtag atgttgcgca
    42961 cgacatgtta cataataaaa ctggtttagg aaatactttt ttaggatgga tagaccttcc
    43021 taaaaattat gataaagaag agtttgacag aataaaaaaa tctgctgata agataaaatc
    43081 agactcagat gttcttttgg taataggtat aggaggctct tatttaggtt cgagagctgc
    43141 tatagatatg gtaagtcatt cttttaggaa tggacttaaa aaagagcaaa gaaaggctcc
    43201 agaagtatat tttgtagggc ataatataag ctcaacatat attatggatt tattagatat
    43261 aattgaagga aaggatattt ctgtaaatgt aatatctaaa tcaggaacta ctacagaacc
    43321 agctctggct tttagaatat ttaaagatta cttagagaaa aagtatggta aggaagaagc
    43381 aagaaagaga atttatgcaa caacagatgc aagtaaaggt gcacttagac agttagctac
    43441 agaagaagga tatgagactt ttgtaatacc agatgatgta ggtggtcgtt tttctgtatt
    43501 gacagcagtt ggattacttc ctattgctgc tgctggactt gatattgatg ctatgatgaa
    43561 aggcgcaaat gatgctagag aagcatttca aaatcctgac ttaaaaagta atgatagtta
    43621 tagatatgct gttgctagaa caattttaca tagaaaagga aaagatgtag agttattagt
    43681 gaactatgaa cctcaactac attatgtaag tgagtggtgg aaacagcttt atggagaaag
    43741 tgaaggaaaa gaaaataaag gtttattccc tgcttctgta gatttctcaa cagatttaca
    43801 ttctatgggt caatacatac aagatggaaa aaggctttta tttgagacag tacttaatgt
    43861 tgaaaactgt aagagaaata taactataag ttcagaggaa gtagacttag atggtttaaa
    43921 ttatttagca ggtaagactg tagattttgt aaatcataaa gcttttgaag gaactctatt
    43981 ggctcataca gatggaaaag tacctaattt agtaataaat atacctcaac tagatgagta
    44041 taattttgga tatttagttt atttctttga gaaggcttgt ggaataagtg gatatttatt
    44101 gggagttaat ccatttgacc aaccaggagt agaagcttac aagaaaaata tgtttgcttt
    44161 actaggcaaa ccaggttatg aaaaagaaaa ggaagagcta gaaaaaagac ttaaataatt
    44221 ttaaatagtt aaggtttaat taagattaaa ataatataat gaaatcatca aaaaggagtt
    44281 tactcaaaat agatgatttc atttttttta tgaaaaattg ggaaaaatat attttgataa
    44341 tttatattat attaagatag ggagtggtat gagatgtcaa gaagaaagaa agggataagt
    44401 ttagtaatat tagttgctat aataagttca atacttagta gttttctaac aataatttta
    44461 gttaaggaca atttagtaag caaatctaca ggaagttcta cgccgatagt tgtaaatgat
    44521 gatggtaaga gtcaaaatat ttatcaagca gttgcagaaa aagctacgcc atctgttgta
    44581 ggtattacaa caactagtgt tgatactagt aatatgtttg caataccaac tgaaacacaa
    44641 ggtgtaggaa ctggaatcat agttgattca aatggatata tacttactaa ttctcatgtt
    44701 atatcagatg gtcaagctac aagtgtaaat gtattattca atgatggttc aacaacttca
    44761 ggtaaagtag tatggtttga ccaacaactt gatttagcta tagttaaggt tgataaaact
    44821 ggactaacac cagcagaatt tgctgatagt gacaaagtaa aagttggaga tatatcaata
    44881 gcaataggta atcctcttgg attagatttt caaaaaacag ttactcaagg gataataagt
    44941 ggattagata gaactataca aacagaaaaa actaatatga ctggtttatt acaaactgat
    45001 gcaagtataa atgcaggaaa tagtggagga ccattgttaa atcaaaaagg tcaagtaatt
    45061 ggtataaaca cagcaaaagc ttctcaagca gaaggattag gatttgcaat accaatcaat
    45121 acagctaaga gtatagttga agaagtaatc aaaaatggaa aatatgaaaa agtaacttta
    45181 ggcataaaag gtacagatgt atcaaattat gaagcagcaa caggtacaaa attaagtact
    45241 gataaaggtg tttatgttgc agaagtaata tctggttcat cagcagaaaa agctggagta
    45301 aaggtagggg atattataac taaagtagga gatacagata taacaggtat gaatgattta
    45361 aataaaaagt tgtatacttt ctcaaaaggt gctagtacaa aaataacagt aaatagaggc
    45421 ggaaaagcag taacaataaa tgtgaatttc taaatatgac caaaaagata gagactacat
    45481 ggtaaagtgt agtctctatt tttatttaat ttttatagta tatacatcta aattaattaa
    45541 aatgatgtat tgtttatagt ttattgctat attttcttat aaaaataaat ttaataaata
    45601 t
//
LOCUS       DAMJWU010000022        44603 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMJWU010000022 DAMJWU010000000
VERSION     DAMJWU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.022"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            158..3704
                     /locus_tag="PC460_001361"
                     /pseudo
     CDS             158..3704
                     /locus_tag="PC460_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            3889..5823
                     /locus_tag="PC460_001362"
     CDS             3889..5823
                     /locus_tag="PC460_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF2600254.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5929..6924
                     /locus_tag="PC460_001363"
     CDS             5929..6924
                     /locus_tag="PC460_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2600255.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6896..7681
                     /locus_tag="PC460_001364"
     CDS             6896..7681
                     /locus_tag="PC460_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2600256.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7669..8367
                     /locus_tag="PC460_001365"
     CDS             7669..8367
                     /locus_tag="PC460_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600257.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8490..8963
                     /gene="ybaK"
                     /locus_tag="PC460_001366"
     CDS             8490..8963
                     /gene="ybaK"
                     /locus_tag="PC460_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF2600258.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9160..9999
                     /locus_tag="PC460_001367"
     CDS             9160..9999
                     /locus_tag="PC460_001367"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF2600259.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10012..10560
                     /locus_tag="PC460_001368"
     CDS             10012..10560
                     /locus_tag="PC460_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF2600260.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10626..11807
                     /locus_tag="PC460_001369"
     CDS             10626..11807
                     /locus_tag="PC460_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF2600261.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPAPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            11975..12361
                     /locus_tag="PC460_001370"
     CDS             11975..12361
                     /locus_tag="PC460_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2600262.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDTDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12511..12774
                     /locus_tag="PC460_001371"
     CDS             12511..12774
                     /locus_tag="PC460_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2600263.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            13300..13983
                     /locus_tag="PC460_001372"
     CDS             13300..13983
                     /locus_tag="PC460_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600264.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGKFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13994..15235
                     /locus_tag="PC460_001373"
     CDS             13994..15235
                     /locus_tag="PC460_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2600265.1"
                     /translation="MKIFSNKDIKIFFLMLISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIILNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISVNGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSIFTISFLKLTNM"
     gene            15307..15990
                     /locus_tag="PC460_001374"
     CDS             15307..15990
                     /locus_tag="PC460_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600266.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15987..18317
                     /locus_tag="PC460_001375"
     CDS             15987..18317
                     /locus_tag="PC460_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600267.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDNGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANIGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNGNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAVSGCLLGSILGLILHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPIKKICELDIIDSINAQ"
     gene            complement(18498..19340)
                     /locus_tag="PC460_001376"
     CDS             complement(18498..19340)
                     /locus_tag="PC460_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2600268.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            19635..20369
                     /locus_tag="PC460_001377"
     CDS             19635..20369
                     /locus_tag="PC460_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2600269.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGRPIEYTISIFRTDKYQYEIVLSE"
     gene            20379..21512
                     /gene="nagA"
                     /locus_tag="PC460_001378"
     CDS             20379..21512
                     /gene="nagA"
                     /locus_tag="PC460_001378"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2600270.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLNNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            21611..22360
                     /gene="nagB"
                     /locus_tag="PC460_001379"
     CDS             21611..22360
                     /gene="nagB"
                     /locus_tag="PC460_001379"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2600271.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            22512..22784
                     /locus_tag="PC460_001380"
     CDS             22512..22784
                     /locus_tag="PC460_001380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600272.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            23444..24148
                     /locus_tag="PC460_001381"
     CDS             23444..24148
                     /locus_tag="PC460_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600273.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAISRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            24150..25637
                     /locus_tag="PC460_001382"
     CDS             24150..25637
                     /locus_tag="PC460_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2600274.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            25842..27455
                     /locus_tag="PC460_001383"
     CDS             25842..27455
                     /locus_tag="PC460_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2600275.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(27818..28648)
                     /locus_tag="PC460_001384"
     CDS             complement(27818..28648)
                     /locus_tag="PC460_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2600276.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            28721..30448
                     /locus_tag="PC460_001385"
     CDS             28721..30448
                     /locus_tag="PC460_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600277.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSHFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            30485..32347
                     /locus_tag="PC460_001386"
     CDS             30485..32347
                     /locus_tag="PC460_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600278.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            32680..33369
                     /locus_tag="PC460_001387"
     CDS             32680..33369
                     /locus_tag="PC460_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF2600279.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYQIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            33474..34160
                     /locus_tag="PC460_001388"
     CDS             33474..34160
                     /locus_tag="PC460_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF2600280.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            34177..36084
                     /locus_tag="PC460_001389"
     CDS             34177..36084
                     /locus_tag="PC460_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF2600281.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPIEVGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            36535..37224
                     /locus_tag="PC460_001390"
     CDS             36535..37224
                     /locus_tag="PC460_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600282.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            37660..38199
                     /locus_tag="PC460_001391"
     CDS             37660..38199
                     /locus_tag="PC460_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600283.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            38218..39261
                     /locus_tag="PC460_001392"
     CDS             38218..39261
                     /locus_tag="PC460_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600284.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            39263..40099
                     /locus_tag="PC460_001393"
     CDS             39263..40099
                     /locus_tag="PC460_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600285.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            40204..40878
                     /locus_tag="PC460_001394"
     CDS             40204..40878
                     /locus_tag="PC460_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600286.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            40878..41930
                     /locus_tag="PC460_001395"
     CDS             40878..41930
                     /locus_tag="PC460_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2600287.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            42160..43008
                     /locus_tag="PC460_001396"
     CDS             42160..43008
                     /locus_tag="PC460_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2600288.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            42998..>44603
                     /locus_tag="PC460_001397"
     CDS             42998..>44603
                     /locus_tag="PC460_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2600289.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLNKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKVLIFIVSISVL"
BASE COUNT        17642 a         4600 c         7762 g        14599 t
ORIGIN      
        1 gattttacaa ataaatctta agctatctta tttaattcta atacaggaag aaggtaaatg
       61 tatattgaaa aaatcataga aatagaagta aacaatttaa ataaatacaa aggtttgcaa
      121 aagaagtaat ataattatat acaatgagga tgtgagtatg atgaacagac taaagccaag
      181 aattttaggt attagtatct tgtttattat tataattatt ttaacatgtg gagctgtttt
      241 caagtttaaa tttaaattag ataataaatt aaaagaaacc acacatacat atttgtctga
      301 gtttacagaa cacaatataa ttaatctcaa gacaagactt agtggtcaat ttgacatgct
      361 tgaatctatt gctaacttca taggaaactt gaataatata aatgatgaaa tggtgattga
      421 tttaatggat tctgatgtaa agcgaagttc tttaattaga atgactatct ctacattaga
      481 tggggaatgt tattctaatg atggaattac ccataatgtt caagatagag attattttaa
      541 aaaagctaaa aatggagaaa agaatatatc agagccttta aaatctattg ttaataataa
      601 agaaataata gttttatcta ccccagttta taaaagtggg aaaatagttg gtgttttaag
      661 tggaatttat gattcaaaaa agttaaatga attattagga ttatctgcat ttgatgaaaa
      721 agcagatgtt tttgttgcaa agaaaaatgg agatataatt gctcaaaaaa caaataaaaa
      781 tagtaagacg atatcatgta tatttcagct ttttgacaaa gataattgtg atgaagttga
      841 ttgtctaaaa actataaaag aaaatatgaa aaatgataag tctgagtatt ttaattataa
      901 cttagataat gaaaaattta tgttaaacta tcagccactt ggagttaatg attggtatgt
      961 tttttctgtt gtgccaaata aaatagtgtc tattcagtca gagaatatat cttatgatgt
     1021 atatattttt gcatgaaata ctactagtta tattaatttt tataatatat atagcatgta
     1081 ttataaataa taacaccaaa attattataa atgaaaaaga aaaatttaaa gtactcactg
     1141 ataatatact aggaggggtt aaaacctgtt taaaagatga atatatgact atgacatatg
     1201 taagtcaggg gtttttagag cttacaggtt actcttgtga agatttaaat aaaatattta
     1261 aaaatagatt taaagacatg atttataaag atgatttaga caagtttaat tgtattattc
     1321 taaaccaatt agaaaaacaa gataaaattg aagttgaata tagaattgta agaaaagatg
     1381 gaagtataat ttggattttg gagagaagta agtctataaa tgataataaa ggaagtgagt
     1441 gtattcaggg agttttaact gatataacag aatttaagaa aatacaagaa gagttagatt
     1501 caaaaagatt tgaaatacag gctatcaatg atagtattgt tggtggaata gtgataactg
     1561 agataaatga tgattttgat agtatatatt taaatgaagg ttatttaaat atgattggat
     1621 atacacgcaa acaactgaaa gatgagctaa ataataaatt aatcaaactt atttttaaag
     1681 atgatagaac agaattaatt aatagtataa acaaacaact caaattaagt gatacatttg
     1741 catctgaaca aagagttgaa aaaagggatg gcactattat atggatttta ttaaaaggaa
     1801 gaaagatgat ttatgataat ggaaaatctg tactttgttg tgttattgta gatattactt
     1861 ctgctaaatt attagagcaa gaacttaaaa agagtgaaga acgtcatgaa atcattctaa
     1921 agcagacgca aaatattata atagaatggg atatagaaaa agataaaatg aatatatcac
     1981 ctaattggta taataagttt aaatatgacc ctataactga aaacgcaaca aaaaatataa
     2041 ttaaatcagt agatatatat catgatgata tggataagtt tatttcatta attaaactta
     2101 cttcacaagg tagtgagtat gaagagtgtg aaattagact tagaactaga gatactattt
     2161 acgcttggtt tagtataaag gcaacaactt tatttgataa taatggtaaa ccttataaag
     2221 tagtaggaag tttgactgat attgatgaat caaaacgaga agctgaaaag ttgaaagaaa
     2281 gggcagaaaa agaccctctt acagatttgt ataataaaaa agcatcacaa tcacttatag
     2341 aagagtacat aacattggat aaaaaaagaa aaggtgcttt aatgattatt gatattgatg
     2401 actttaaagg aataaatgat aatttaggac atttatatgg ggatgctgta ttaagtgaag
     2461 ttgcaaatga cctaataaat ctatttagag caaccgatat agtaggcaga attggtggag
     2521 atgagtttat tgtatttata aaagatattt ctaaaacaag agatattata aataaagcag
     2581 aagaattggt aaaaacattt gaaaggtctt ttttaggtaa aggacgaaat tataaaatat
     2641 cattaagtgt aggaattgct aagtttccac aagatggtat ggactatatg acactattta
     2701 gaaatgcgga tatagctctt tactcagcca aagggaaggg caagaattgt tatacattgt
     2761 atgataaaaa aattgataaa gtccaatata taactaatac aagaaatgca ggagatagtg
     2821 ataatgtaga gatgtctttt aaagaacata tcacaaatta tatatttgat atactatatg
     2881 aatcaagtga tatagaagtg gcagtaaatt taattctaag tattgtaggt aaacacttta
     2941 atgtgagtcg catgtatata tttgaaaatt cagatgataa tcgagtatgt aaaaatacat
     3001 ttgaatggtg taacgaaggt ataacctcgg aaattaataa tcttaaaaat atttgttaca
     3061 aggatttagg caactatctt ttaaatttta atgcagatgg gttattttat tgtaatgata
     3121 ttgaaaattt aggtagtgat gtgaaaaaag tacttaagcc acaaggcata cgttcaatat
     3181 tacaatgtaa aattttagag ggtaatgaat ttagagggtt cataggattt gatgaatgtt
     3241 catataatcg tatgtggact aatgatgaag tgaacacttt gacatttata tctaaaacgc
     3301 tcaatatgtt tttattaaaa atgcgtattc agagtaagct agaagagtct ttaacaattc
     3361 aaaaatctgt gatggataat atggatatat gggcatatgt tgttgacagg aatacctatg
     3421 aattactatt tatcaataaa aaaatgcttg agtcagcacc aaacactcat gtaggtgatt
     3481 tatgttataa agcgatatgg aataatcaag ataaaccttg taaaaaatgc cctatgatat
     3541 atttaaaaga tggtgatgat acttataaat cattcatgca taataaaaag tttgaggtac
     3601 aggcaaatat aactacaact actttgaaat ggtttgatgg taaagatgca tgtttaatat
     3661 ttggaagtga aataactagt attgaagctg aagaaaatac ttaaaatact gtgttatata
     3721 tatacttaaa atcgcaattt gaatttatta gacttaaaat gtctatataa atcatttaag
     3781 ttgtggtttt aagtattttt tatgatataa ttaagcgtta agaacaaaat taaaagatac
     3841 ttttgtataa aaaaatattt gttaaatata tattgagcag gtgataatat ggaaaataag
     3901 tttttaccaa taagcaaaca agatatgata gatagaggat gggaagaatt agattttgtt
     3961 ctagttacag gagatgctta tgtggaccat catagttttg gaacagcaat aatatctaga
     4021 gttttagaaa gtgctgggta taaagttgga ataatagctc aaccagactg gagaactaca
     4081 gatgatttta tgaagctagg aaaacctaga cttgcatttc tagttaatgc aggaaatatg
     4141 gattcaatgg tcaatcatta ttctgtaagt aaaaagcata gagaaaagga tatgtattct
     4201 ccaggtggga aaatgggtta tagaccagat agagctacta tagtatattg caataaaata
     4261 agagaagcat acagtgatgt tgcaatagta ataggtggaa ttgaagcgag cttaagaaga
     4321 tttgcacact atgattattg gagtgataag gttagaaagt ctatgttaat agatagtgga
     4381 gcagatttat tagtttatgg tatgagtgaa aagcagattg tagaagtagc caatgcactt
     4441 aatgatggat atgacccaaa atacattaga catattgatg ggacatgcta tatttcagat
     4501 acattggaag aaatatatga taaatatata ttaattccat catataaaga aatttgtgaa
     4561 gataaaatga agtatgtaga agcttttaaa attcaatatg atgagcaaga cccatttaga
     4621 ggtaatataa ttgttcaacc tcatggaagt aagtatctgg ttcaaaataa gccagaaaaa
     4681 ccacttagta gagaagaact agatgaagta tatggattac catatcagaa gacataccat
     4741 cctgtgtatg aaaaatttgg aggaatacca gctatagaag aagtaaaatt tagtatagta
     4801 agttcaagag gctgttttgg tagttgttca ttttgtgcaa taacatttca tcaaggtaga
     4861 gcagtgcaaa gtagaagtga aaaatcaatt attgatgaag caataggaat aacaaattta
     4921 gatgatttta aagggtatat acatgatgtt ggaggtccaa cagctaattt tagaagacca
     4981 gcatgtaaaa aacaaattac taaaggtgct tgtaaaaata gacaatgctt atctccatca
     5041 ccatgtaaaa atttagatgc agaccacagt gaatacttac atttacttag agctgtaaga
     5101 aaattaccta aaataaaaaa ggtgtttgtg cgttctggaa ttagatacga ttatgtaatg
     5161 gcagataaga acaataaatt tcttagagag ttgatagagc atcatgtaag tggtcagtta
     5221 aaggtagccc cagaacatat atcagaagaa gttttaaagt atatgcaaaa accagcagga
     5281 aaaacttatg ataaatttag acagaaattt tttgctataa atgaagaatt aggtaaaaag
     5341 cagtatttga taccctattt aatgtctagt caccctggtt ctaccttaaa tagcgctata
     5401 gagttagcag agtatttaag agatactcac taccaaccag agcaggttca ggatttttat
     5461 ccaactccag ggactctatc tacaaccatg ttttatacag gtattgaccc gcttacaatg
     5521 aagccagttt atgttcctaa atcaaaacgt gacaaggcaa tgcaaagggc tttattacaa
     5581 tatagagcac ctagaaatta tgatttggta tactctgcgt tagttgaagc aggaagagaa
     5641 gacttaattg gttttggaca tagatgtctt ataaagccaa aaaatgaaaa gccttatttt
     5701 aatagaaata attctaaaaa aaatgtatct aaaggaacaa ataaaaacaa aaaaactaat
     5761 acaaataaca gaagtaataa aaatcaacaa aaaagttcaa ctaaaaaaaa gaaaaaacat
     5821 taaagtataa ataaaaatac ttaaaaaaga aggattattc gataaattaa cagaaattag
     5881 tataaataaa tataattatt ttaatgacgt tacctttggg aggaagagat gaaaaagtat
     5941 atttctataa tattattagt ggtgttgact atggttgttt tggtaggatg ttcaccagga
     6001 aaagataacc ctaaaaataa ggagctatca gttgtcaaac aagtaaaagt agctgttcca
     6061 gatggactac cagcagtggc aatagctaaa cttgcaaatg aaaatccaga gattaaagaa
     6121 ggatatgaaa cagtgtatag cattgaaaag acacctgaag cgatttccac tagagtgatg
     6181 aaaaaagatg cagatatagc tatagttcct tcaaacatgg ctgcaatagc gtataataaa
     6241 acttcatcat ataatattgt tggaactgta ggaatgggtt cactttattt ggtgtcaaca
     6301 gaaaatataa aagattactc tgatttagtt ggtaaagaag ttggatgtac tggaaaagga
     6361 cttactccag acataactat aaaaagtctt ttgttacaaa aggatataaa ttattcagat
     6421 ataaaattta attatgtaaa ttcagcaagt gaactggtac cattattggc tactggtaaa
     6481 gttaaaacag gtattgttcc agaacctgca cttagtgctt tgatgtctaa aaatccagac
     6541 ataaaaatta taaaaagttt aaatgactca tggaaggaag taagtggttc aaaagatggt
     6601 tatcctcaat ctactttaat tgtaaagtct gaatttttaa gagataataa agattttgtt
     6661 gattcttttg taggtcaatt atcaaatagt atagattggg caaacaaaaa tcctgaagaa
     6721 ttaggtgcat attctgaaaa aataaaaata tctactgaat caaagataat aggaaaatct
     6781 cttgaaagag ctaatttgaa atatattcca gttaaggaca tgattaaaga ttataaaaat
     6841 tactatgaga aattagctaa ttttgatgat aagacattag gaggcaaagt accagatgaa
     6901 gcaatatact ttgttgagaa ataaaaaaaa taaagaacaa tttatagata aaatggaggt
     6961 aacactttct tgtgttatcc tcttattttt atggcaaatt attgctttaa aaattgataa
     7021 tgatattttt ttaccaacag taggtcaagt gtttaatagt ataaaagaga ttatgctaaa
     7081 ttctagtttc tatatagata tactttactc tatggggaga tgttttttta gttttttact
     7141 ggctatgatt tttgctatta tactttcaat agtgtcttat ttaaatagat tttttagaaa
     7201 cttattaaag ccaatcaatg ctttgacacg ttcaatacca actatgatat tggtagtact
     7261 tgcacttatt tggtttgaaa aggatagtac cccttttata gttggattta ctatagtttt
     7321 tcctatattg tatgataacg ttttgggcgc tatattgaat atagacaaga acttattaga
     7381 aatggctaat gtgtataaaa taaggtttat agataaggtg ttaaaaattt atttaccagc
     7441 cataaaattt caaattatat ctatactagt atcaacattc tctttaagtt tgaaagttgc
     7501 tatagcagga gaggtatata gtcaacctac atatggtatt ggaactatga tacagactga
     7561 aaaaattaac tttaacacat ctggaatatt tgcatggata attattgttc ttttaatatc
     7621 tgctatttta caaatagctc aaaagttttt agcaaggaga gcttttttat ggaaaagata
     7681 aagttgagta ttgaaaatat aaataaaaga tatgatagtc gtattatatt tagagatttt
     7741 aatattgatt tttatgtaaa tgaggtaaat tgtatccttg gaaaatctgg ttgtggaaaa
     7801 actacactct tgaacataat aagtgggatt attaaaaatg acaccaacaa tctaaatatc
     7861 aaagaaaatt taaatagagt aggaaataag cttgaagcta gttacatatt tcaagatgat
     7921 agacttatag actggttaac agtagaagaa aatataaaga tagttgtaaa taaatactat
     7981 aataaaactc aattaaataa aatttgtgat gaatacttgg aattggttgg aattagtgat
     8041 tataaaaaat tctatcctca aatgcttagt ggaggtatta gacaaagagt aaatattgct
     8101 agagctttta tatatccatc aaaaaacatt ataatggatg aaccttttaa atctattgat
     8161 gcaaagaata cacagcttat aatggataat ttcagaaata tattaagaaa agaaaagaga
     8221 actgtgttat ttgtaactca taatattgag gaagcattgt ttttagcaga taggatattt
     8281 attttaggag actctccaat tagaataaaa aaaatattaa aaaattctaa ggaattggaa
     8341 aaaaatgaag ttttgaagtt gatatagagt tataataata ctgtcgtttt aaaatatatt
     8401 tggttaaaat ctataagact atattaaaat agtacagaat attcaaaact gtgtataatg
     8461 ttcgtatttt ataaaaagga agtgaatgta tggttaagac taatgctatg agaatattag
     8521 attcaaataa gattgattat aaagtaatgt cttatgaagt gaaaagtgaa catgtggatg
     8581 gtgttgaggt ggcacatgat ataggaagag atgtaaatga agtgtataag acattagtaa
     8641 ctcaaggagt tagtaagaat atatatgtat atgtaatacc agtacatgaa aacttagact
     8701 taaaaaaggc tgcaaaagta gctaaagaga aaagtgtaga gatgattcat gtaaaagata
     8761 taaataagct tacaggttat attagaggcg gatgttctcc tataggtatg aaaaagcttt
     8821 ataaaacttt tgtaaatgaa agtgcaaaga atttagatac aattattgtg agtgcaggaa
     8881 aaataggata tcaaatagaa ctatctccat ttgatttaca aagacttata aaagtagaat
     8941 ttgtagatgt aataaaaaaa taagaattac atataaaata tagagcttat attctcaata
     9001 ataggtgttg taatattaat aaaatgaata ataattttaa aataaatatt cagttactta
     9061 tgtgtaattg gtataaaaat attgagaaat tttaaaaaca atggtaaagt gtaagtataa
     9121 tcacgaatat tttttataaa gtaatggagg gatgttcgct tgagaaaaat aaaatctgaa
     9181 caaatcgtcg agcaggtaaa aaaactttgt atagaagcga gcctatattt aggagaagat
     9241 gttttaagtt gtataaaaga aaaagcgaaa agcgaaaaga gtgaagttgg aaaaaatata
     9301 ttgaatatat tagttgaaaa tgctgaaata gctaaagaaa aaaatatacc aatatgccaa
     9361 gatactggta tggctgtatt ttttgttgaa ataggacaag aagttttaat tgaaggagac
     9421 acacttactg atgctataaa tgagggagta agacaagggt atgaagaagg ctacttaaga
     9481 aaatctgttg taagtccaat taacagagta aatacaaaag ataatacacc tgctgttatt
     9541 cattatgata tggtaaaagg tgataaaatt aaaatagaat ttgcagctaa gggatttgga
     9601 agcgaaaata tgagtaagat gaaaatgtta aagccatcag atggattgga aggaattaaa
     9661 aaattcataa tagatacagt ttcagaagct ggacctaacc catgccctcc tatggtaatt
     9721 ggagttggta taggtgggac tgtggacaaa tgtgctcaaa tagctaaaaa ggcacttttt
     9781 agagaactag gtgaatttaa taaggacgaa aatatagcaa aacttgaaag tgaactatta
     9841 acagctatta ataaacttgg tataggacct caaggcttag gtggtactac aacagctttg
     9901 ggattaaaca tagagacttt tcctacacac atagcaggtc tacctgttgt tgtaaatata
     9961 aactgccatg catcaagaca taagaaggta gtaatttagg aggaaaataa aatgataaaa
    10021 ataactacac ctgtaaatga aattgatata gcaaaattaa attgtggaga tacaattagt
    10081 ttgagtggta tactttatac agcaagagat gctgctcata aaagattgat agattgtata
    10141 aataaaggtg aagaattacc atttgacgta tatggtcaag gaatatatta tgtaggacct
    10201 actcctacaa agccaggaga agttataggt gctgcaggtc caacgacgag ttatagaatg
    10261 gatgatttga ctatacctct tttagaaaga ggtcttagat tgatgatagg caagggaaaa
    10321 agaagtgatg aagttataga aggtatgcaa aaatacggag cagtttatct tgctgcaata
    10381 ggaggagctg gagcgtatat ttcaaattca ataaaatcat gtgaaattat tgcatatgaa
    10441 gatttaggag ctgaagctat aagaaagatt gtagtagaag accttaagct tacagtagca
    10501 atagatagct atgggaataa tatatatgag caaggtcgag caatttatga atgtaaataa
    10561 tgtttagaaa taaatatgaa tatcaaaatt aaaaatagtt gtgtatattt taaggaggaa
    10621 aattgatgtc taaggattat gcaaaattgg cattagaaat gcatgaaatc aacaaaggga
    10681 aagtaagtgt agaaagcaag gtagaagtta aaactaagga tgatttatca acagcatata
    10741 caccaggggt tgcagaacct tgtttaaaaa tacatgaaaa tcaagatgat gtttatagat
    10801 acacttctaa agggaatcta gtagcagtag ttagtgatgg aagtgctgta cttggattag
    10861 gtaacattgg tgctgaagca tctatacctg ttatggaagg taaagctata ttgtttaaac
    10921 aatttgcaga tgtagacgct tttccaatat gtttaaagac taatgatgta gatgagatag
    10981 ttaaatctgt agagttgatg gaacctgtat ttggaggtat aaatttggaa gacatatctt
    11041 ctccaagatg ttttgagata gaagagagat taaaaaaatc tttaagtata cctgtttttc
    11101 atgatgacca acatgggaca gcaataatag ttgcagcagc cataataaac tcaataaagt
    11161 taattgaaaa taaaaaaata gaagatttag aaatagttat aaacggagca ggagctgctg
    11221 gtatagccat agcaaagata ctacttaata tgaatgttaa aaatataatc ttatgtgata
    11281 gaagtggagc attggaagct agtatagaaa atctaaacta tgttcaaaaa gaaatgttaa
    11341 aagtaacaaa tataagaaat gaaaaaggac ctttaaaaga tattataaaa ggaaaagatg
    11401 tatttattgg tgtttcaggt ccaggagctg ttacaaagga aatggttgaa agtatgtctg
    11461 agaagccaat aatattggca atggcaaatc ctgctccaga gataatgcca gaagaagcaa
    11521 aattaggtgg agcatttata atgggaacag gtcgttcaga cttccctaat caagtcaata
    11581 atgtattggc ttttcctgga atatttagag gagcattaga tgttaaggct aaagagataa
    11641 atgaagaaat gaagatagct gctgcatatg caattgctaa tacaataagt gatgaggaaa
    11701 tatgcccaga gtatatatta ccagatgtat ttaataagaa tgtagtaaaa aatgttgcta
    11761 aggcagttaa agaagcagct ataaaaacta aagtaaatag aatataaatt tttatatgct
    11821 catatattct tacttttata tacatatctt tatgattaaa cgtgttagaa aacgtagtaa
    11881 atttatacta tcagacttta cctttattac ttaaaatgat ataatatgaa tattataaat
    11941 taataaattt aaatttattg aggtgcaaga gataatggaa tataaagtta atgaccaaga
    12001 acttaatgct tcaatgttta tcccattcgt taacaggata tggccaggag attatgatac
    12061 ggataaaaca cagactgcat tatcaaaaac tttgaatata actgcttatg atgataaagt
    12121 gcttgtagga tgtctgcgag ttctttcaga tggttattat tttggaacaa ttacagaatt
    12181 gcttgttctt ccagagtatc agaaacaagg cattggtagt aagcttttcc agcttgctaa
    12241 agagaataca ccaactatgc tatattttgg tgcacagcca ggagtagaag agttctatga
    12301 aaaaaatgga tgccaaagaa gtcttcaatc ttacataata aaaaagaaag atagttatta
    12361 aagttccagc tttcaataat cttaaaagta caatatttta gtggaatcat ttcatttttt
    12421 attactaata tgacataaac atgtcatatt agtattgata tacttttaat atggttaaga
    12481 gccaaagaaa aatataagga gtgatttaaa atggaagaaa ctaaattttg ccaatgctgt
    12541 gcaatgccaa tgggacaaac tgatgaatta tatgggacaa ataaagatgg aagtaaaagt
    12601 actgattatt gtagttattg ttttaaagat ggtgagttta cagctgatat aagtatggaa
    12661 gagatgatag aagtttgtat ccctcatatg cttcaaagta ataaggatat gactgaagat
    12721 gaagcaagaa caatgatgaa caatttcttt ccaacattaa aacgctggaa ataattttat
    12781 gcaaaattag ttatatgcaa aacatgaaat atggtgttga gaataagggg tggtgtgctt
    12841 tatagcatac cacctgtttt gttacttgct agttcaggta ttcttgtatg cttaaatatg
    12901 ttctcatctc tctttctatt ttaaattgta aaaatctaaa atatgttatt tatattctac
    12961 ttctttatcc atgtcttttt ctaccacaag tcaaatccaa ctacaaaatc atcattttca
    13021 attcactgtt taatagaatt aacagataaa gagttataaa attttctatt ttttatataa
    13081 aataatacag gtcaagctga cttctagcta cttgggatag tgtatatatt tatgatagtt
    13141 ataggagtaa taccaatcat tagactgttg atttgtgtag tcttttgtaa aaacatttgt
    13201 tttaatatat tgagtttttg aaaaagtaaa attataatta taataaatta agtgtaataa
    13261 tgtattagaa gtaaaaataa ttaaaataga ggtgattaaa tgagtagtat attgttagtt
    13321 gaagatgatt taagcttgat atatggatta gagttctcta tacaaaaaag tggatttagt
    13381 atagacatag caaggacagt aaaagaagct atgcaaatgt atgaggaaaa aaattatgac
    13441 ttattgcttt tagatgtatc cctacctgat ggtaatggat ttcagatttg cgaaaaagta
    13501 cgagaggttt ctaatatacc cattatattt ttgactgctt cagatgaaga ggtaaatgta
    13561 gtgatgggtc ttgatatggg tggagacgat tatataacaa aaccttttaa attaaatgaa
    13621 ttaatttcaa gaataaaagc attacttaga cgttataatg ttgcaagtaa tgtgaatgaa
    13681 ttaagttcaa ataatattac tattaaatta cttgaaaatc gtgtttttaa aggtaaattt
    13741 gaggtggaac ttaccacagc agaatataaa ttattgtgct tatttatgaa aaataaaaat
    13801 attgtactta ctagaaaaaa catattggac aagttatggg atggaaatgg tagctttata
    13861 gatgacaata cattatcagt atatgtaaga cgattgagaa ataaaattga agataatcct
    13921 gaaaatccta agtttctatt gaccgttaga agaatgggtt ataagtggaa tgtaattaag
    13981 tgagggtatt aatatgaaga tattttctaa taaagatata aaaatatttt ttttaatgct
    14041 tatatctatt ttagtaatgt ttattatagt agggcaaata gtggttataa gtataactaa
    14101 caattataaa aaaacgttga tagagcatga ttataatata gctggatact tatatagtat
    14161 aggtgttgat aaatctaaaa taccatcagc atttgttgca catgaagata aatatatttt
    14221 aaaaggaaaa gaaatactag atacagctgg atatggattt gatatagatg ttagctttac
    14281 accaagtgta aaattgtttc aaagtaaata tataaaaact atgtctatct atttattagt
    14341 attttttact atgattattt ttatactata tatgtatttt tttaggcaac aaagtaagat
    14401 agaagaggct agttataaaa ttgatttatt tatgaatgga aatataaatg aaagactaaa
    14461 tacatatgaa gaaggtagct tatcaaaatt atttagttca atagatgtta tgtcgacatc
    14521 tttaaataca catatattaa aagaaaaaca gagcagagaa tttcttaaaa atacaatttc
    14581 ggatatatcg catcaattaa aaactccact tacagcttta ataatgtaca atcagatact
    14641 acaagaagaa agtcataata gtgatgtcgt tgaagctttt gtacaaaaaa gtcaaagtga
    14701 actagaacgc attgaaacat tgattaagaa tttattaaag attacaaaga tagattcaaa
    14761 aacaataatt ctcaataaaa ataacgttaa tcttaaaaat ttaattgtca aaattttgta
    14821 tagctttgaa actagggcag aaaaagaagg aaaggttatt tctgtaaatg gttcagaaaa
    14881 tacattgttg ttctgcgatt atgaatggat gtcagaggct ttaagtaatc ttataaagaa
    14941 tgcattagat aatacaaaag agaatgataa aataacgata gagtggtttg agacgactat
    15001 aaccaccatt gtttcagtta aagatactgg aaatggaatc catgttgagg atattcatca
    15061 tatatttaaa aggttttata gaagtaagtt ttcaaaagat aatcaaggtt taggactggg
    15121 attaccacta gttaaatcta ttgtggaaca acataatggt actatttctg tagaaagtaa
    15181 actatcacaa ggaagtatat tcaccataag ttttttaaag cttactaata tgtaagataa
    15241 aatacatctt gcagtaaggt atatagatta ttattatatt aatcaattag aaaggtgaga
    15301 gagaaaatgg atattcttgt ggttaataat ttatgtaaaa catatggaaa tggatataca
    15361 aaggtagagg cattgaaaga tgtatccttt acagtttcaa aaggagagtt tgtgactgta
    15421 gttggagaat ctggttcagg aaagagtaca ctattaaatc tcttaggagg tcttgatact
    15481 ccaacatcag ggagaatatt tattgatggt aaagaaatat gttcaatgaa agaggaaaaa
    15541 cagactattt ttagacgtcg taacataggg tttatatttc aggcatataa ccttatacca
    15601 gaactaactg tggagcaaaa tataatattc ccattgttat tagattataa aaaaccagat
    15661 actatttact tagatgaagt attgactatt ttaggtctta aagataggaa aaatcatctt
    15721 ccaaatcaat tatctggtgg tcaacaacag agagttgcaa ttggaagggc tttgattaca
    15781 aagccaagta ttatactggc agatgagcca acgggaaatc ttgacagtaa aaatagtaat
    15841 gatgtaattt cacttttaaa agtgactgca gaaaagtata atcaaaccat tattatgata
    15901 actcataata cgaatatagc ttctacagca gatagaatac tacaggtttc tgatggtgtg
    15961 cttacagacc tgggggtgaa tagagaatga gaagctactt agaattagta ccacaatatg
    16021 caaaagtaca taagaaaaaa aatagaacta caattttatg tattattatt gcagtatgct
    16081 tagttactgc tatctttgga cttgcagata tggctataca atgtcaaaag atacaattta
    16141 ttaaagatga tggaaattgg cacatagtaa ttagaaatat tgatgaaaag tttatatcag
    16201 aaataaatag tagagtagat atagctcacc atggtagggg gcaagtcgtt gacaatggag
    16261 agtttaaatc aaaggagatt ttgatacaag gtttagatga agaagtagca aagcaattta
    16321 acttagaaat aataaggggt cgttatccta ttggggagaa tgagacttta atagatgagg
    16381 atgcagttaa aagctttaat ctaaaaatag gagattttat caaaataaaa ttagcagatg
    16441 gtagaaataa aaattataag atagtaggtg aatattcatc aatatcaagc ttaagagcta
    16501 cagataaaca tggattaata ctttcaatat tgggtacaca agctattaaa aaatcagaaa
    16561 cgtacgatta ttattatatt acatttaaag aaaaggttaa tattagaaaa gccacttctg
    16621 atattaaaaa taccttcaat ttaagtgata aacaaattgg taaaaatgaa agattacttt
    16681 ctattatagg tcaaggtgac tctgactttt ctaaaccatt gtatttaaca gcaggattac
    16741 tttttgtact tgttttagta gcaagtatta tcatgatata taatagtttt aacatgagtg
    16801 taattgagag agttaaattt tttggtctgt tacgttgtct agggtcatca aaacatcaag
    16861 ttaaaaaatt tgttgtttta gaaagtttaa ttttaagtat aaaagctatt ccaataggct
    16921 tagtattagg aagtcttgta actattatat cttctatatt tcttaagtat gttaatgagt
    16981 tattcagctc aatgccaatt ttaaaagtta gttttattgg tattgcattt ggaataattg
    17041 tagggttttt aactgtaata ttatcagcta ttattccagc aaaaaaagct tcccgtgtat
    17101 ctccacttag cgctgtacgt gggaatctaa tgtccaaaga aattttaaat aacgggaaga
    17161 caagtaaagt tgcaaatata ggaagtttta aaattgagac ttctatggga ttaaatcatg
    17221 ctttttcaag aaagaaatct tttgtactta tgacttcttc atttgccatt agtattatac
    17281 tatttttagg ttttagtgtg cttatagacc ttatgtatca aggacttaag cctatgaagc
    17341 cagaagctcc tcatatatct attgttgacc ctaattttaa gtctgtttta actaaggaag
    17401 atattaatga aataagcaat atcaaaggag taaagaaagt atacagtaga atgatgacgg
    17461 atatagaagc tacatataat gggaagaaga caaaatcaca attaatttct tatgaaaagt
    17521 atcaatttaa ttgggcaaaa aaatatctat taaaaggaga agttaatgaa aataaactac
    17581 agtcagaaaa ccttgcatta gtagaagagg ggcatggatt tgatatagga gataagatat
    17641 atattaaaaa tggaaatgct gaaaaagagt tagaggtagg aggtatactt tcctcaattc
    17701 catttgaggc taaagataaa tatattggaa atattataac atctgaaaat acttttacga
    17761 atataacagg tataaagggt tatataataa tggatattca ggttgataaa gatactcctg
    17821 gagatttaat tgaaaatata agacgtgtag tagatgataa cttagaggtc agagataaaa
    17881 gacaaagtaa cttagaggtt aaaaattcgt tttatactat ggcaacattt gtatatggat
    17941 ttgtgtttat tattgcgatt ataagcatgt ttaatatcat aaatagcatg aatataagtg
    18001 ttacaagtag aaagaattac tatggtgtta tgcgtgcagt tggcatatcc aacagacaat
    18061 taagaagaat ggtaatagta gaggcaggtt catatgcagt gagtggttgt ttattaggta
    18121 gtatactagg attaatatta cataggtata tgtttatatc gttagtaagc tcaatgtttc
    18181 atttagagtg gcaagtacct tttaatttac tacttatcac tgtactaact atgataataa
    18241 taactttatt atcagtaaga aaaccaatca aaaagatttg tgaattagat ataatagact
    18301 caataaatgc acaataaata taggtattga tttaaattaa aaaaacgaag taaaaatgaa
    18361 atagaaaata tttttcattt ttacttcgtt ttttatatta taaaattttt gttatgttac
    18421 aaaatttcta taaagatgat gtgaatctaa acattatatg acaacgtgag catttaagtt
    18481 aaaatatttt aaatatacta ttttagataa gttccctctt catcttgata aattaatcct
    18541 tttttcataa gcactccaag agctcgttta aaatttttct tacttgaatt aaataatata
    18601 gaaatatctt tagggtcaga tttatcatta aatctcatat atccatcaga accttctaaa
    18661 taacttaaaa tcttattttc taaagtatca agttcatcct ttctatttcc tctaggagaa
    18721 agaccaagtt ttccatcttc atatattttt atgacatgta aattttcaag aacatcacca
    18781 gtttttagtt cagtaaagta ctcattatga agtattacac cagcatattt attgtctaca
    18841 catatcattg cagaattatt agtttggaat ccatatacaa ctccagttac aatatctcct
    18901 acattgtaag tatggtctgt taataagtat gaatctatat ctgtagtagc agcaattctt
    18961 ttgcttttgt ctaaatatat ataaaataaa tatttttcgt ctctaaatag tggataagtc
    19021 ttagctttaa aaggaactaa tatgtcttta ggaagtccca tatctataaa agtaccaatg
    19081 tctgtattat caactacttt taaatgtgct acatccccaa ccttagctaa aggcggtatt
    19141 aaagttgcag cagttctgtc atcagaatct ttaaatatga aagcatttac ttcatcacct
    19201 atgtttattt cattttttcc gattaatcta ttatgaagaa gtatatcgtc ttttgtgtta
    19261 ttagtttgtc catccaaaaa atatccaaat tcagtttttc tttttactgt aagtttatta
    19321 aaatcaccta tgtttatcaa attaaaatca tcctctcatt ttatatttat aatttagcca
    19381 taatatactt atgtagtagt ttgtcacagt atctatatta gtattagtga aatttacaat
    19441 aaataatata gttatttatg tatttttcaa ttctctagct tcttatatag tataaactcc
    19501 aatttctttt ttgtctttag gttttattaa aatttttaca aaataagtta aaaagtattg
    19561 aaataaaatt tcttttaaag tataattggt atataccaaa attggtatat accatatcga
    19621 aaagtggtga ctatatgaaa atagtaaata aatctagtaa tataccattg catactcaat
    19681 taagttcaat aataagagag atgatagaaa ctggtgagct taaagagggc gatgcaataa
    19741 tgccagaaag agaactttgc aacatacaaa atgtaagtcg tatgacagta aataaaacca
    19801 ttgttggatt agttacagaa ggacttcttt atagggtaca aggtaaaggg acattcgttg
    19861 caaagcaaaa aaagaagtat cagttttcaa atgtaaaagg ttttactgat gttatgaaag
    19921 aaaagggagt aaacataaaa acggatatat tatcctttga gatggaacta ccagatgatt
    19981 tagttaaaag aaagcttgga ataagtgata atactacaaa tatatataag attgtcagac
    20041 ttagatatac tgatggagag ccatttggat tagaaatagt atatctatca gaagaaatgt
    20101 gtaagggtct aacaaaaggg atattggata attcttcatt atatagggtt ttaaatgaaa
    20161 aatatggcta taagatacaa aaagctgaac aggtaatgga gccagtaata ctttcagatg
    20221 aagaaagtaa gcttcttgaa acagatgaag gtgctttagc tcttaaactt catagaaatt
    20281 cctataatag agaaggtaga cctatagaat ataccatatc aatatttaga acagataaat
    20341 atcaatatga aatagtattg agtgagtagg tgaataatat gaaatgttta attaatggaa
    20401 agataatatt aaaaaatcaa atcttagaaa ataaagtatt agtatttgat gaaaaaataa
    20461 ttgatatagc tgatagtgta ccaaaagatt gtgaagttat agatgctgat ggaaaatata
    20521 tatctccagg gcttattgat attcatatac atggaaacat gggaaaagat acaatggatt
    20581 cgactgatga atctattgaa acaatttcaa aatcaattat gagacatgga gtaacatctt
    20641 ttcttccaac tacaatgact atggataaag aacatgttta tgatgcactt gaagttatta
    20701 aaaaagccca aaatagaaag cttgaagggg cacaagtatt aggagcacat ttagaaggac
    20761 cttttataaa tgagaattat aagggagcac aaaatgaaaa atttataata aattcaaaat
    20821 acgaatttat aaaagaatat aaagatgtta taaaagttat aacttatgct ccagaaaaag
    20881 atattgattt tgattttact agagaaataa aaagatgtac agatatagtt ttatctattg
    20941 gtcatagtaa tgcaaattat gaccaagcta aagaagctat aaatttagga gtaactaatg
    21001 taactcacat gttcaatgca atgactggtt taaaccatag agacccaggc gttgttggtg
    21061 cagcacttac aactaatgtg tacagtgagt taatagcaga tacaatccat ataaataaag
    21121 atttatttca atttatacta aataataaag gaaaagaaag actgatttta ataacagatt
    21181 ctatagaagc tggtggtctt gaagatggca attattctct tggaggtcag aaagtcatag
    21241 ttaagggtaa tgaagctaga cttgaaaatg gagcattagc tggaagtgtg ttgtctttaa
    21301 ataaaatggt atttaacttc ttaaataata caaaccttaa agtgaatgaa gcaattaatt
    21361 tagcttcttt aaatccagca acatctttgg gaataaatga caaaaaagga agcttagaaa
    21421 taggtaagga tgcagatata gctgtatttg atgagaattt agattgtaaa atgacacttt
    21481 gcttggggga agtagtatat aaaaatatat agtatacaaa acaaacctta aagacagtaa
    21541 aaacaatata aaaaataata aattaaactt acattagtaa aagtagtagt taaaatttag
    21601 gaggaataag atgagagtat tagtgtgtaa agattatgat ggaatgagta aaaaagccgc
    21661 tgaaatgata gcagctcaaa tagttttaaa accaaattca atactgggac tagctacagg
    21721 aagtacacca gtaggtatgt atagagattt agtaaaaaaa tacaatgata atatagtaga
    21781 tttttcagat gtgatgagct ttaatttaga tgaatattat aagttaccaa tatctaatga
    21841 ccaaagctat gattatttta tgaaagagaa tctatttaat catgtaaaca taaagccaga
    21901 aaatactcat ctaccaaatg gaatggctga tgatattgaa aaagagtgta tgaactatga
    21961 agcttctata gatgcagctg gtggtataga tgtacaagta cttggaatag gtcgtaatgc
    22021 tcatataggt tttaatgaac ctgatactaa atttgcaaaa agaactcatg tagttgaact
    22081 tacagaatct acaatagaag ctaatgcaag attctttaaa tcaagagaag atgttcctaa
    22141 aaaagctgta agtatgggaa taggttcaat attgaagtct aagaaaatat tgcttttagc
    22201 ttcaggagaa gaaaaagctg atgcagtata taatactgtt tacggagata taactccaga
    22261 agttcctggt tctatacttc aacttcataa agacactata gttatagtag atgaagcagc
    22321 agcaagtaaa ttgaatccaa aagattataa attagtataa gaatagaatg tagtttaaat
    22381 aaaaaatagc tatcattgaa taatcaaatt tatttaatga tagctatttt tatttaaatt
    22441 agttattatt gtttaaatta cttgcttatt actttttacg aaaactcgtt atcaagaagt
    22501 atccaaataa gatgttaaaa attattatgt ttagtgggat tctatcaagc ataataaaat
    22561 tacctataat tgggatatta ccaactggaa acataaagaa gaatacaggc atcagtgata
    22621 gtaaaaaaga tggaaaacaa agaagaagta atcttggaat atcaatatac caactaccat
    22681 cctttttgta ttctctaatc aataattcag ttcctagtaa taaaccaata ccaccagagg
    22741 aaattaaatt aataaaaatg tatcttttaa agtcttgcat ataaccagtg taaaattgag
    22801 tatagaaatc ataatttaat ttaacggcta aaaaatacat aagcaataga atgattaaaa
    22861 ataaaagata tctaagagct tttttcattt taaattctcc cttcagaaat actttattta
    22921 gttttatttt gttggaatct gattttatca tttagtttaa gaaatccata atattagtga
    22981 agaatgaaat aagtattata tatgaataaa tatttttaga attaaggtga attgtagtgc
    23041 ttaatataac tataatatta attgtttgaa tattcaataa aaagttcgaa aaatatcaat
    23101 tctgatataa actgaataaa tacagttgaa tattaaatta ttattttatc aaaaatatgc
    23161 aaagtactac accttcaaat atcacctaaa gtaattaaaa ggcaattaaa gtgaagtagt
    23221 tcaagtattt ctaaaataag tttaataaat tctataataa tttatttttt gccttaaaat
    23281 tgccttaaaa tccaaacaat tttaaaacta atgtactata aaaagctggt ataatttaaa
    23341 tatagtaaaa aataagattt atcttatcta ttgggattta attatttaat caatatattt
    23401 ataccattta atttaaataa aaagtctatt aaggagtgag cttatgagta agttaattta
    23461 tatagcagat gatgaagata atataagaaa tttggtaaag acattcttaa aaaatgaagg
    23521 tcatgatgtt atggatttta aaacgggtga tgaactttta gaacaattta atataaagga
    23581 gtgtgactta gtaatactag atataatgat gcctggctca agtggttttg aagtatgtac
    23641 aaaacttcgt gagaaaagta cagtcccaat aatcatgctt actgcaagag atacagatat
    23701 agattatatt acagggataa ccttaggtag tgatgattat tttacaaaac catttagccc
    23761 aatgtctttg gttatgagag taaaatctat atttagaagg atagagtttg aaaagaaaca
    23821 aaattatgat aaatatagta actcgataga tatggaatta aaatttggtg atgtaataat
    23881 aaataaaaag aataaaatcg ttacaagtaa aaatgtaaat atagacctta caccaaatga
    23941 atataaccta ttgacttatt tgtttgaaaa tatagataga gcaatttcca gagatgaatt
    24001 attaaacaaa atatggggat acgatataga agttgagaca agggctgctg atgatacagt
    24061 gaagagatta cgtaaaaaaa ttttagatac aaatatttta atagaaactg tatgggggtt
    24121 tggatttagg ttgaaggaga aaagttaaga tgaagaatgg aaatgacaag aatagaattg
    24181 gaaagattaa atccaaattt aaattaaagt tctcaaagaa aaatataagc ataaaaacta
    24241 ggattttgtt gagtatactt tgtttgataa ttatagtatt tatgataata ttagtatctt
    24301 ttaatgtatt tcttgataca tatataaaaa caactgcgaa tgaagaattg acaaagtcta
    24361 cagaaatagt agaacatatg gatagtaatt ttagacctat taagccacca caagatgatg
    24421 gaaagcttcc tcccaaagta ttatctaatt ttatgagaaa tgtacaagat aaagttagga
    24481 tggcagaaac acaatcagat gctgatgcta tggtagttga ttctaagtat aacctgatat
    24541 ttccaacaag agaggacgat ttcttaaaaa atgttgatga gatggagctg attagggagt
    24601 gtattcaaaa tgaaaaatta gatttaaatt cagatgcaaa tacacggata tctaccagaa
    24661 ataaggatta ttatatttca actgtaaaaa ttaccagtat atatggtgaa caagacaagt
    24721 atcttatact ttttatagac atatcaaaaa ctctaaatct agcacaaaag ataaatattg
    24781 tattaatatc tgtaatgtgt tttgctggaa tattagcaat atttacagct gtgattttat
    24841 ctgagaaaat tgctaaaccg ataaaagagt tgtgtgagtt tgcaaaaaaa atgggtcaag
    24901 gagatttcaa aagaacctac tttgactttt cagataaaga attagtagag ctctcaactg
    24961 ttatgaataa gagtgctgag tatctggata agtatgataa tgaacaaaaa atattttttc
    25021 aaaatgcatc tcatgaatta aggacaccac ttatgtctat taaaggttat gcagaagcta
    25081 taaaatataa tgttatagat agtaaacatg caagtgatat aattcttgaa gaaagtgata
    25141 ggttaagtga tatggttgaa gatttattat acatttctaa aatagataat ataactaaag
    25201 attttgaact tgtagaatgt gatttaagag aagtactttc aaactgtgga gctaggcaaa
    25261 atgtaagagc tataaataag gggataaagt ttatttatga atttgatgaa gaaatggttt
    25321 tatttgaatg tgatgaaaaa aacatttcca aagcatttat gaatcttata gaaaatgctt
    25381 tgagatacgc taagagtgaa ataaaaatag tgtgtaagta taatcaaaaa aatatagttg
    25441 tgataataga agatgatgga attggaatca agaaagaaga tttacctcat gtttttgaaa
    25501 gattctataa gggagtagga ggaaatcatg gaattggatt atcaatagtt aaatctattg
    25561 taaataagca tggtgggcgt atatacgtag aaaatggcaa aaaaggagct aaatttacta
    25621 tattatttaa attgtaaaaa aattagagaa aatatagaga tgtttgaagt tgttttttat
    25681 atatatctgt tgtatactgt tgaaaaaaga acaatattaa gctgacatat attacgatag
    25741 ttgacattta aagtaaatgc ttaaatatag atacgaaagg aaaagtaaaa tctgaagtgt
    25801 atttaggttt aagtaagctt cttattttat aaaaaatatg tatgaaagaa cagctgattt
    25861 ttaatattac aaatttagta acggttgtat ttgaagcttt tgtcatccat atgtttttat
    25921 cagacttttt aggccagaaa gaagggtata taaaagttgt aagatatgct aaactgggtt
    25981 ttataatatg tttaggattc tgtaatttaa taactttaaa ccctaaaatc acaatgcctt
    26041 taattttcat ccttattttt tcaacatcat ttttatataa aggaaattta aagacacgat
    26101 tgtttactac tgttttatta agtatattct ttatactgtc agaaattgta gttacaagta
    26161 tttttgtttt atttgtaaaa gaaggctttg aaataatgct tgaaaataat agtataagag
    26221 tattggcgac aattttatct aaaattgtct ttttgttgac ttgtaaaatt atttgtctat
    26281 ttaaaaaaga tgtacattta gatatgccca taaagtattg gctcccttta tttttaatac
    26341 ctatatttag tttatttctg tctgtttcta tctttgatgt aagtaaattt tttagtcttg
    26401 aaagtttaaa atttctaagc ttaatatcta gtgttggtat tttatatatt aattttattg
    26461 tcttttattt atttaaattt attatagata aaactaaact atctatgaaa tatgaactac
    26521 ttgaaaaaga aataattcat aaggaagaat taagacttag taatgagtgt tataaaatta
    26581 ttgttgaaca gactgattct gtcgttttcg aatggaatat caaagaaaat aaatcatttg
    26641 tatcgcaggc ttggactgaa aaatttggat ataacaatgc atgtaagaat atttttaaag
    26701 aaattaaaga taaagactta gttcattctg aagataaagc tatatttgaa ggttttcttg
    26761 aaagtattaa aaaaaagaat atgcacaatc aagcagtata tcgtttaaaa aaatctaatg
    26821 gtgaatatat ttggtgtaga acatcaatta caagcattta taatgatgaa aatgaacttt
    26881 taagagttgt cggagtaatt gtagatgtag atagtgatat aaaaaaatat gaagagttaa
    26941 gaactagagc agaaagtgac tcattgacca atatatacaa taaaggtaca tttgaaaaat
    27001 tagtggaaga aactatagtt atgaatacag gtgataaaaa agatgcatta ttcattattg
    27061 atttggatga ttttaaagaa ataaatgata attttggtca tccatttggt gattttgtac
    27121 taaaaacatt tgctgataag atacaaacta gttttggtag taaagactta gtcggaagaa
    27181 ttggtggaga tgaatttgtt gtatatatgc aagattatgt tacagaagta aatttgcata
    27241 aaaaagctaa agaattaaat agagtacttt cggataatta cactgatttg agttttagtt
    27301 ttgatgcttc tgtcagtata ggtattgcaa ggtatcctca agatggaact tcattttttg
    27361 aattgtttaa aaatgcagat agagcacttt atagcataaa agcatcaggt aaaaattcat
    27421 attgtttatt tgaagaagaa ctatatgtac agtgaatttg aatttagatt ttatgtgaga
    27481 gtaagattta tatatacttg tggctgtctg atttataaaa tatgttactt tgtatatttt
    27541 tatatgtttc agtaaggtta attaacataa gcaaaggtgt aagtaaattt atattagttt
    27601 aagtttactt acacctttat aaaaatacaa ataatagatt catttacatt cttaaatgat
    27661 taaagctctt taccttctaa acatgcataa catatcgtta taattccagt aaataagact
    27721 aaaataccca ttcctaagat tacagtttca ttatctatta ctccaaatgt ttttaaagta
    27781 acttatttcg ctttgtatca taataatttg tctcttttta cattttaact tctgctgaga
    27841 tttgagttag atattcgtta gaatctcgca cacttatatc atatagaaga tattcttcat
    27901 aaacaaaatc cccaatacgt aaattattct gattaaaaaa atttaataac ttttcataag
    27961 ccttgtaaat agtttcataa ctccctttat gataagcagt tacataaagt ccctttggtt
    28021 tgattgatac aggatatttc ttgtactctt tattaacttt tgtaaataaa tatgaatatg
    28081 attcaaagtc tttattaatt atatcttcac ctttaatgat tgagccaatc gtaccataat
    28141 cactcaattc atagttgtcc ataaagttaa tacattcata taaaaaattt tcttcctcat
    28201 ctcctaaaaa ctgttcttta tatgtaacag gtgttttgac caagtactct tcttcatgtt
    28261 cttttaaagt aatagtattc gcatctatat tacaggcatt tttagtgaat gaaatagtag
    28321 attccattag agcttgaata ttatttagtt tttcaatttc atttttaata tcaatggttt
    28381 tcttttctaa aagctcaatt agtaaattag gtgttctttt gtctatataa gctttaattt
    28441 cttttaaagg catttttact tccctaagtg aagaaattat accaaaaatg ctaagttgat
    28501 ggtatgagta gtatctatat ccattttctt tctttaattc tggtgagaaa agacctatat
    28561 catcataatg gaaaagtgtt tttttattga ttccacaaat ctttgcaaat tctccagttg
    28621 taaaataatt ttttgaaact tgactcataa ttataattcc tccttgacta tcctgttaca
    28681 gtatagttta tcatagtatt ttgtaaagga gtgtgattat atgaaaaaat taattcattt
    28741 tttaaaacca tatagagttt taattgttgt agttttgata tttacgtttt tacaaacact
    28801 tggaactttg tatataccaa cacttacagc aaatatagta aacaatggtg tagtaaaggg
    28861 agatatagat tatattgtaa aaacaggttt aatgatgatg atagtagctg gtataacagc
    28921 tttatcagct gtgttagttt gtaaagtatc agcaaatctt tcatctggat tttgtagaga
    28981 tattagagaa gcagtattta taaaatcaca agatttatca ataaatgatt ttaataatat
    29041 aggtacagca tcaatgatta ctaggagtac tagtgatatt actttaatag gtcagtctgt
    29101 gtttatgttt attcaattag tattaccagc accaattata actgtttctg gtctattctt
    29161 agcatattct atagataaag caatgacaat tattattgtt gtagtaatgt ttttattcat
    29221 gctttcagca tttttagttg gtaaaaagct tataaagttg tttaaaatga tgcaaataaa
    29281 aatggacaat atgaataggg tgttacgtga ggtagtaact ggagtaagag ttataagagc
    29341 ttttaatagg agtcattttg aaaaaaagag atttgataga actgcaatag attattctga
    29401 aacagcaatt tcaataaata aaatatttgc agtacttatg cctattgtaa tgttaataat
    29461 gaatttaggt atagtaagta ttatttggtt tggtgggatg agagtcagta atggaaatat
    29521 ggaaataggt catataatgg cacttgttga atattgtatc ttaattttat tctatttaat
    29581 catgggagta atggtattta tgtacatacc tagagcaggt gcatgtgcag atagagtaaa
    29641 tcagatatta gatatagaac ctgaaattgt agatggaaat ggtcataaag atactgtctc
    29701 agagagagga catttagtat ttaaaaatgt aaccttttca tatgcacaat cagaagaacc
    29761 tgtgttaaat aatataacat ttgaagcaaa atctggagaa gtaactgcaa ttataggaag
    29821 cactggctca ggcaaatcta ctattgcaaa tataatacca cgtttttttg aaattcaatc
    29881 tggagaaata tctataaatg gtcaggatat taagaagatt cctcaaaaag aacttagaga
    29941 taagattgga tttgtaccac aaaaagcttt tttatttagt ggaacaatag aagaaaatat
    30001 aaggtatgga aaagaagatg caagtattga agaagtaaag catgctgcaa gtatagctca
    30061 agctgatgaa tttatttctg atatggaaga caagtatgac tcatttgtag cacaaggtgg
    30121 aaataatttg tcaggtggtc aaaaacagag aatatccatt gcaagagcgt tagttagaaa
    30181 gcctgaagta tatgtctttg atgatagttt ttctgcatta gattttaaaa cagataaaag
    30241 acttagaaaa gcgttgaaaa atgaaatcaa agattcatca gctataatta tagcacaacg
    30301 tataagtaca atcatggatg ctaatcagat tatagtttta aatgatggaa aaattgtagg
    30361 tattggaaaa cataaagatt tacttgaaaa ttgtgaagtt tataagcaaa ttgcggattc
    30421 acaacttagt aaagaagaac ttgcataaaa tgaagaatta atctgatatt tggaggtgta
    30481 tgatatgagt gaaaaaagaa tagatgaaca agatatagaa gtacttagtt ttgaagatgg
    30541 catgtcagca gaaaaagcta aggatgcaaa aaaaactact aagagattat taaaatatat
    30601 ggcaaaacaa aagtttaaaa tattaattat ttttatttca gtattgataa gttcagcgct
    30661 tacagtttta gcacctatgg ttatgggaaa agctattgac caactattta atggaataaa
    30721 aactgcagtc caaacaggta caaaattcag tgtgaatttt agtactatgg gggggatagt
    30781 atcaatactt ctagggttgt acttaattag tgcagtattt atatatattc aacagtatat
    30841 tatgtcagga gttgcacaaa atctagttct tagcatgaga aaagatttaa gtgataaact
    30901 caataaactt cctcttaagt actatgattc tcataaaaag ggggaaacac ttagtatagt
    30961 aacaaatgat ttagaaaaag tagcagatag tttacaagaa ggattaatgc aacttattac
    31021 agcagtagtt acagtagttg gttctattgt aatgatgata agtataagtg taccacttac
    31081 aatagtatca gctataacat tacttgtttc attgggtatt actgttgtaa ttgcaagaaa
    31141 atcacaagaa agattctctg aaaatcaaaa ggcactagga gagttaaata gcaatataga
    31201 agagatattt actggtcaaa tagtggttaa agcatttagt aaagaaaagg atactataag
    31261 aaactttaaa atattaaatc aaaatcttta taatgcaagt agaaaagcac agttttcgtc
    31321 atatgcaata tctccaatta ttagatttat aaacctaatt ggctatgtta ttatagcagt
    31381 tgtaggtggg atatttgcat caactggagc aatgactctt ggtagtatac aagcgtttat
    31441 tcaatatgta aatcaagcat cagagcctac tacagaaata tcttatattg taaatatgct
    31501 tcaagcagca atagcatctg cagagagagt ttttacagtt atggacgagg tggaagagat
    31561 aaaggatgaa gaatcttcaa aggttattag catgcctaaa ggtaaagtac aatttgagca
    31621 tgttaaattt ggatatagtg atgattttat tcttatgaaa gatattaata tcaatctaaa
    31681 tgcaggagat aagattgcaa ttgttggacc tacaggagct ggaaagacaa ctcttgtaaa
    31741 tttacttatg agattttatg agatacaagg tggaagaatt actattgatg gtgttaatat
    31801 aaaagattta aagcgtggag aacttagaac tatgtttggt atggttcttc aagatacatg
    31861 gctgtttaat ggaagtataa aagaaaatat tgcatatagt aagtcagatg ccacaatgga
    31921 tgaaattgta agagcagcaa aatcagcaag agtagaccat tttataagga ctttaccaca
    31981 agggtatgac actatattga ctgaggatgc ttctaatcta tctcaaggtc aaaagcaact
    32041 tctaacaatt gccagggcaa tactttcaga cccatcagta ttgattcttg atgaagcaac
    32101 atctagtgta gatactagaa cagaggtaga aattcaaaag gcaatgaata acttgatgaa
    32161 gggaagaact agttttgtta ttgcacatag attatcaact attcgagatg ctgatttaat
    32221 attagtaatg aaagaaggaa ctattattga acaaggaagc cataaggaac ttattgaaaa
    32281 gaaaggattc tatgaagaac tttataacag tcaatttaca agtgaatatg atgaagatgt
    32341 tgtatgatta tcaagtttta ggcaactaaa tgattgataa atattaagtt aaatatagta
    32401 acatgtaagg ttatatggaa taagagtatt tctctatttc atataacctt ttttatttat
    32461 caaaataaga taaaatttcc tttctaaatt agattgaaga atgtatttat caatataaaa
    32521 gtaaaataat cattattatt agtgaagtaa atattgtttg ccacatatat gccataaaga
    32581 tgcacttatt ttacggagat ttaacaaagt agtgtaaagt atactctaaa tataaaataa
    32641 atcaaataga gttaaataaa ttgagaggtg atataaatag tgaataaaaa attgaactat
    32701 agatttgaaa tgaagcacaa aataactgaa gcagatgtgt tagctttaaa atcaagactc
    32761 tatcaaataa tgaagaaaga tgaaaatgca tctaaagatg gaaaatatct tattaggagt
    32821 ttatattttg atactcctga agataaagca cttttggata aattaaatgg cgttgcaata
    32881 agagaaaagt ttaggataag attttataat aatgactgct cttatataag gctagagaaa
    32941 aaaatcaaac attacaacat gacatcaaag ctatcaacta atttaaccaa aaaagaagta
    33001 ataaatatac taaataatga tattgaattt ctaaaagagt cgacaaattg tttattaaga
    33061 gaattttatt tgaaattaaa atgtgaaaga ttagaggcaa aaagcatagt tgattacaat
    33121 agagaagctt ttatttatcc agttggaaat gtaagagtta caatcgatag tgatattaaa
    33181 acttctgtaa attcggtaga tttatttaat aaagatttgc caactgtttc ggtaattgat
    33241 gagaatatga ctgtattgga ggttaaatat gatgaattta taccagattt tataaaggat
    33301 ttaattcaaa ttaataagac tacctctaca gcagtttcaa agtatgcttc aagtagatta
    33361 tatatttaag aataaaggct atgatttata taaagatata cataaataag gttaaaaaac
    33421 aaataagtta aaaataaata aaataaaaaa taaataagtt aaaaatagga aggatgattt
    33481 taattatgac atttaatgat atttttaaat caagttttat agaaaacgta tcaggttttt
    33541 catttgtgga cagtgcttta gcactaggtt cagctttttt agtaggtttg tttatatata
    33601 tggtctataa aaagacttat atgggaatta tgtattctcg accattcaat gtgtcgcttg
    33661 tagcacttac aatgcttaca acttttgtaa tattggcagt tacttcaaat gtagttttat
    33721 ccttaggtat ggttggtgct ttatctatag taagatttag aacagcaatt aaagacccaa
    33781 tggatttagt atttctattt tggtcattgg gttcaggtat agttttagga gcaggtttga
    33841 ttcccttagc tgtcatgggt tcaataatta tgggattgat tctaatattc ttttcaaata
    33901 aaacaatatc tgaaactcca tatatattga tggtaaattg taataatgaa gattcagaag
    33961 atatagcaac tgataaaatt aaaaaagtat ttactaaata tcaaataaaa tcaaaaagtg
    34021 ttacaccaga aaaaggaata gaattagtgt ttgaagtaag aatgaaagat ggagaaacaa
    34081 gtcttataaa tgacttgagt caagtagatg gagttactaa tgcagttctt gtaagttata
    34141 atggtgacta tgtagcatag agaggagtaa tcttttatga aagataaaaa atttaccctt
    34201 cttatctcga ttatgattat atttttatgt gctgtagttg gagtttatag tacatctagc
    34261 aacaaaagtg ttgatttata tagtgatgta tatattgaaa aatattttaa cagagacaag
    34321 gttatggaag ttaatataga gatagatgaa agtgacttga aggatatgaa tgaaaatgct
    34381 ataaaagaag aatttaaggt tgcaaaagta actgtagatg gagatacata tggaaacgta
    34441 ggtataagaa ctaaaggaaa ttcaagtctt atatctgtag caaatagtga tagtgataga
    34501 tacagctata agattaattt tgataagtat aatactagtc aaagtatgga agggcttact
    34561 caattaaatc ttaataactg ttactctgac ccatcttata tgagagagtt tttaacatat
    34621 agtatttgcg aggaaatggg attagcgact ccagaatttg catatgctaa agtctctata
    34681 aatggcgaat atcatggttt gtatttggca gtagaaggat taaaagagtc ttatcttgaa
    34741 aataattttg gtaatgtaac tggagactta tataagtcag atgaaggaag ctcgttgcaa
    34801 tataaaggag atgacccaga aagttactca aacttaatcg ttgaaagtga taaaaagaca
    34861 gctgattggt ctaaaatcac aaaactatta aaatctttgg atacaggtga agatattgaa
    34921 aaatatcttg atgtagattc tgtccttaaa aatatagcaa taaatacagc tttattaaac
    34981 cttgatagct atcaagggag ttttgcccat aactattatt tatatgagca agatggagta
    35041 ttttctatgt taccatggga ttttaatatg tcatttggtg gatttagtgg ttttggtgga
    35101 ggtagtcaat ctatagcaat tgatgaacct acgacaggta atttagaaga cagacctctc
    35161 atatcctcgt tattaaaaaa tgagacatac aaaacaaaat accataaata tctggaagag
    35221 atagtaacaa aatacctaga ttcagactat ttagagaata tgacaacaaa attgcatgac
    35281 atgatagcat catatgtaaa agaagaccca acagcatttt atacttatga agaatttgaa
    35341 aaaaatataa catcttcaat tgaagattct agtgataata agggatttgg taataaaggg
    35401 tttgacaaca ataactctaa taacagtgat tctaataata attctaatag tgaaaataag
    35461 cgctctggaa atcaaagtga tgaaaaagaa gttaatgctg aattaacatc aagcgtagtc
    35521 aaagctaata cagataatga aactaaaaat aaaactacaa atgatagtga aagtaagaat
    35581 aatacagata aagataaaag tggaaatgat aataatcaaa agctagaagg tcctatgggt
    35641 aaaggaggta agtcaatacc aggggttttg gaagttgcag aagatatgag taaaactata
    35701 aaatctcaat taagtggaga aacttcttcg acaaagcaaa actctggtga tgaaagttca
    35761 agtggaatta aaggtagtga aaagtttgat gaggatatga gtggtatgcc agaaccacct
    35821 gagggaatgg atggtaaaat gccaccagga atgggtaata tggataaggg agatatgaat
    35881 ggtaaaaatg gcaatatgaa tatggataga aatcaagata atccaataga agttggaggt
    35941 tttggtaata gaggaggagg ctctgtgagt aaaacaacaa catacttcaa attaatttta
    36001 ggtggagctt caatgataat aatgtcgatt atgttagtag gtgtatcaag ggtaaagaga
    36061 agaagattta taaagtcaaa ataagattaa aattgaatat aaataatttt aatactgtta
    36121 agtctaaata tttacttagc agtatttttt agaaaataat gtgtttgatt aataatataa
    36181 ggtaataaat tgataaaaat taaatacagc atattaataa tacataataa agtaataaaa
    36241 aactgtttaa gtataataac ttatatttag acagtttttt tcatttataa ataatataaa
    36301 tgaaattgaa aacatttgaa ggatagaaat tggttggata aatgtataaa ttacttcacc
    36361 aaaaaataat gacaaaaact gtaactaagt tgtaataaaa tggtagttat ttagcataaa
    36421 ataatgtatc atagaagtat attataaaat taataaaata agatttttgt aagaaaactg
    36481 ctggcttttg atatttagag tgagtagtta aattatgttt aggaggagta gaatttgttg
    36541 caaaaagtat tatggaaatt tttaaagcta gtagtcggtt tatttatatg ttcagttggt
    36601 atagtattga cgataaattg taaccttgga ttatctccct gggatgtatt tcatcaagga
    36661 ctttcaaatc atataggaat aactatagga actgcttcta taattgtagg ctctatagta
    36721 gttattgctg atgtcgttct tggagaaaat gtaggatggg gaactgtttt taacatgcta
    36781 ctaattggat tttttatgga tttactttta tatagtaatt taattccaga ggctgataat
    36841 ttatttgtag gaataattat gttgatatta ggattggttc ttttgtctgt tggaatggta
    36901 ttttatatgg gaagtggttt aggaagtgga cctagagatg gtttgatggt tgcaattcaa
    36961 aagaaaacag gaaaatcact taaattaata agaggtacta tagaagttgg agcattaata
    37021 gtaggatttt tattaggtgg aaaagttggt ataggaacta taataagtgc atttggattg
    37081 gggtatttca cacaaatggt atttggctta tttaaattag attgtagtaa gattaaacat
    37141 agatttatag tagatgatat aaaatttatc aaagtgtata tgagtggtga gaaaaaagct
    37201 gctgttcaaa atatagttaa gtaatttaag aataaattca acgaaatgtt aagtatttta
    37261 aattaacatt ttgtttagat agataaattg aatattaaaa gaatattaaa atatgaggat
    37321 tgctagaaca ctataaaata tagatttcta gcaatttttt atttgttcaa tattttttta
    37381 gtactagaag tcttattctc tatttaatga atgaattaat ttataattag agatactaaa
    37441 ctttatagat attaatataa acacctttaa actgtgtatt ttccaaagcc tttaaaatgc
    37501 ttgtatttat tctaaataaa aaaataaaaa aatgtcaaaa aaactcttgt attatatcca
    37561 aattttatat ataattacct atgttgttta gaaattttaa actcaatgtt tagtaaacaa
    37621 aatgtttagt aaaataaaac agaaactagg aggtgcaaca tggatatagg tgaaaagata
    37681 aaaagattaa gaacagaaaa acaactgacc caggaagagt tagctaatag atgtgaattg
    37741 tcaaagggat ttatttctca attagaaaat aacttaacat caccatccat agctaccctt
    37801 atagatatac ttgaaatact aggtacaaat ttaagggaat tttttaatga aatagatgat
    37861 gaaagaatca gttttacaaa agaagatatg tttgaaactg aagatgaaga tttaaaatat
    37921 aaattaaagt ggttaattcc aaattcacaa aaaaatgaaa tggagccaat aataataaca
    37981 ttgtatccag gaggacagta taaagaagaa aagccacatg aaggtgaaga atttggatat
    38041 gtattagcag gttcaatata tgtccatata ggcgaaaaga aaaataaagt aaaaaaagga
    38101 gagagttttt actttagacc gaaagcaaat cactatatat caaacgaggg caaaactaca
    38161 gcaaaagtga tttgggtaag tacgccacca tcattttaga atagaggtgt ttaataattg
    38221 gtagaaaata taatagaatt aagggatata tctaaacatt atgaagaact tactgtttta
    38281 gataatttca atttagatat aaagaaaaat gagtttctaa cattgcttgg accaagtgga
    38341 tgtggaaaaa ctacaacatt aaaaataata gcaggatttg agtatgctga tgatggtaaa
    38401 gttttatttg aaggtaagga gataaataat ttgccaccat atgaaagaca agtaaacaca
    38461 gtatttcaaa aatatgcttt atttccacac atggatgttt atgaaaatgt agcttttgga
    38521 ttaaaaatta aaaagactcc aaaagatgta atagatgcta aagtaaaaga ggttttgaaa
    38581 cttgttgcac tagaaggttt tgaaagacga caagttgaat cattgagtgg aggacaacaa
    38641 caaagaattg caatagcaag agctctagtc aatgaaccaa aggtattatt actagatgaa
    38701 ccacttggag cacttgattt aaagttaaga caagagatgc aaatagaatt aaagagaatc
    38761 caaaaaaaat taggaataac atttgttttt gtaactcatg accaagaaga ggctcttact
    38821 atgtcagata ctattgttgt aatgtataag ggcaaaattc aacaaatggg aacaccacaa
    38881 gatatataca atgaacctaa aaattcattt gtagctaagt ttataggtga aagtaacata
    38941 tttgatggaa ttatgataga agattataaa gttaatttct gtaatagaga ttttgagtgt
    39001 gttgataaag gattcgaaaa aaacgaaaat atagaagtag taataagacc agaagatata
    39061 aaaatggtaa agccagaaga aggaatgtta aaggggaaag ttacatctac agtgtttaaa
    39121 ggagtgcatt acgagataga attgaatgaa aatggaagat tgtggatact tcataatact
    39181 aaaaatgctg aggttgggac tgaactaggc atggatatat atccagaaga tatccatata
    39241 atgagaaaag agaataacta agatgaaaag aaaatctttt ttagcatatc catatgtagt
    39301 ttggagtgcg atatttgtaa taattccttt aatattagta gtatttttta gttttacaaa
    39361 agaatcaggt ggaggttatg cctttacact tgagaattat aaagaagtaa ttgaccctat
    39421 atatatgaaa gtttttgggc gttctattct tttagcagga ggagctacat taatatgcct
    39481 tatagttggt tatccagtgg catatataat ttcaaaggct cgtgtaagta gaagaggttc
    39541 attaatatta ttgtttattc ttcctatgtg gatgaacttt cttttaagaa catatgcatg
    39601 ggttgctata ttaggaaaaa atggactttt aaatacattt ttaggttggt ttggaataca
    39661 accattagca attttgtata ctaattttgc tatactttta ggtatggtgt ataacttttt
    39721 accatttatg gttcttccaa tatatacagc actatcaaag atggataatg atttgataaa
    39781 cgctgctcat gacttgggtg caaataatat gacagttttt agaaaaatta tatttccttt
    39841 gagtttgcca ggtgtaatgt ctggaataac tatggtattt atgccagcag tcactacttt
    39901 tgcaatatca agactgttag gtggaggtaa aataatgctg gttggagatt tgattgaaca
    39961 acagtttaca gttgttggag attggaactt tggttcagct atatcgatat ttatgatgat
    40021 agtaatatta atatctatgt ctataatgtc aaaatttgga gatgaatctg acaaagaagg
    40081 cggtgggtta ctattttaaa attaaagaaa ataacttcta atatatattt agcattagta
    40141 ttcttatttt tgtatgcacc catatttgct ttggtactat tttctttcaa tgattcaaaa
    40201 tcaatggcaa gatggaatgg atttacatgg aagtggtatg gtcaattact tcaaaatgag
    40261 agtataatga gtgctttata ttatactatt gtaatagcta tattagcgtc tgtaatatct
    40321 acaatagtag gaactataag tgctatagga atacataaga tgagaggtaa gagtaagaaa
    40381 ttaatactaa atgtaaacta tcttcctatt ttaaatacag agatagttac agcagtagct
    40441 cttatgagtc tgtttgtatt tgtaaaaatg gaatttggat ttacaacaat gctattggca
    40501 catataatgt tttgtttgcc atacgttata ctttcagttt tgccaaaaat gaagcaatta
    40561 cctgataata tagaagatgc agctatggac ttgggagcaa cacctatata cgctcttaga
    40621 aaagtaattt taccacaaat aaaaccaggt atagtttctg gatttttaat tgcatttact
    40681 atgtcaatag atgactttat aataagtttc tttaatgctg gaaatggtgt aagcaatctt
    40741 tctatagaaa tctatggaat ggctagaaga ggtataaaac ctgaaataaa tgcattatca
    40801 acaataatgt ttgcagtggt tctaggttta cttttactag ctaataaaaa agaatctata
    40861 gtaaggggga ttaaataatg attaagttta aaaaagcaat atcccttata gctgtaggta
    40921 tgatgacagt tactttattt acaggatgta ataaagcaac tgattctaca aaagttttaa
    40981 atgtatataa tgttggagat tatatagatg agtctcttat agataagttt gaaaaagaaa
    41041 caggaataga tgttcaatat tcaacttatg atactaatga aatgatgtat caaaaagtaa
    41101 agagtggtag tacacactat gatttagtat ttccttcaga ctatatggta gagaaaatga
    41161 aaaatgaagg tcttttggaa aaattagatt ttaagaatat acctaatatg aaatatttag
    41221 ataagagttt tttaaatcct gtatacgaca aaactaatga atatagtgtt gcatatatgt
    41281 ggggaactct tggaatattg tacaataaga aagaagttaa agaccctatg gatagctgga
    41341 atatactttg gaatccaaag tataagggaa atataatgat gtttgactct gttagagata
    41401 ctatagggat aactcttaaa aaattaggat atagcatgaa tagtgtaaat ccaaaagaaa
    41461 taaatgaagc taaaaatctt ttaatgaaac aaaaggattt agtattggct tatgtaaatg
    41521 atgaaggtaa ggatagattg cttggtggag aagtggcaat gggtatgctg tattctggag
    41581 atgcagttac actaatggag caaaatcctg accttgatta tgctatacct aaagagggaa
    41641 ctaataaatg ggtagatgct atgtgtgtac ctacaacagc acaaaataag aaagaagctg
    41701 agctgtttat aaacttttta ttagacccag agaatgcaaa agtaaatgct gagtacattg
    41761 ggtattcaac acctaataca ggagcactaa aattattaga cccagaaata actcagaatc
    41821 ctgttgcata tccatctaaa gaagtacttg ataaatgtga aacttttgaa gatattggtg
    41881 aaaatataaa attatatgac aaggcttgga tagagttaaa atcaaaatag ggtcttgttg
    41941 ttttaagtaa atttacttaa taaatactta ataaaactgt tcttttttag aaaatttatt
    42001 ttctaattat tgaacagttt tattttttgt ttaagttata ttcatatgca atttaaatag
    42061 tttattaata aatgaaaact tatttgtata atgactttaa taataattag aaaaaatggg
    42121 caacttaata gaataattgg aggtgtaaat ttataaaaaa tgaataggat aaataaaaaa
    42181 atagatttat taatattggc tttgctcgtt tttatatttt taattgttgg aatagtaata
    42241 ctatcgatta ggacagaaaa taaattgaat gtgttcataa tgttagcaat aatttttttt
    42301 ataataatgt taacttattt atcaaattca gtagtggggt tgataacaag ttcaataatt
    42361 atatttatgt atacatccta tattttatat aacaatatta ctcacaatat ggatgttgaa
    42421 tttatatcat atatgtggat aatagcaaca ccagtatctt caattataat gggtaatctg
    42481 aacaaaagca taaatgaatt gcaaaataca aataaaaaac tatctgaaca gtataaggaa
    42541 ttagtgacta ttgatagtga aactggactt agaaatttaa agatttttta taatgatgta
    42601 aatatggaga taagtaaatc tattagacat aatactgatt tttctcttat gatagtaaaa
    42661 ttaccatact acggaaacct acagactata tttggtgaaa acaaaactaa taaaatagta
    42721 aaatatattg gttcaaatat tatagaatgt acaagaaatg aagatataat ttatagttta
    42781 caaaaagata tgatagggat actaatgcca aatacatctt tggaaggttc aaaagttgtt
    42841 aaagacagaa taaagaaaag gataaaagaa ttaaatttag atttaaacaa taggggaaaa
    42901 tatgtaaata tagatgtcaa aattgcattt ttacaatata agagtagttt tggagatagt
    42961 ataaatttta agaatatagt tgaagaggaa cttcaatatg atgtttaaaa tatttaataa
    43021 aagtctatta aaattagtag ttgtattatc aatagtactg acaatatcaa attttagctt
    43081 atcttcatat gcatcagaaa ttacttccag cacagataag gtattaatca tatatgattc
    43141 taagaaagaa acagcttata atagagatat tttaaatatt atgaggactc tattgggaag
    43201 atttagttca gatatagagc tattaaaatt gagtaactat gatggtgaga taaataaaaa
    43261 ttattacagt catatattta ttttaggaat caatgaaaac tcatataata atgataaaaa
    43321 cactaaaaac ttgattagtt ccttaaattc atataaagga actatatgtt ggttaggata
    43381 tggtatagaa aatcttttgg aacataaaaa atacaattta gactatgtag gaaaaacaaa
    43441 caatatagtt agtgtaaatt atagaggtaa atcatataac ttggatgaac attatgtttt
    43501 taacattgta gaatcaaaag atacaagtaa taaagtaata ggaagtatta atgatacctt
    43561 aaataagtat ccatatataa taaatgataa aaatcttttt tatgtatcaa aattagattt
    43621 agatggagtt ttattttata tattttgcga ttcacttaat gatattttta atattaagac
    43681 gtttgataaa ggacgtatat ttgtaagaat agaagatgta catgctttta gagaaccaaa
    43741 aaatcttgta gagatagcgg attatttaag tagtaagaat ataccattta cgattgcatt
    43801 aataccagca tatgtaaatc ctaaaaatca taaggtaata actttatcag aaagccctga
    43861 aattgtcaaa gcaataaaat atatgcaaga taaaggtgga actgtgatat tgcatggata
    43921 tactcatcaa tacaagaaag aagaagtttc tggagaaggg tatgaatttt gggatggtaa
    43981 aaaagatgaa cccttaaaag aaaatatgaa gatatttgtc aaagatagag ttttgaatgg
    44041 tcttagagta tgtatagaga atggaattta tcctcttgca tttgaagcgc cacattatgc
    44101 aatggaatca gagggatata aagaattaaa aaaatatttt tctacttata tggggcaaca
    44161 tcaaaataat gataaaaaat ttagcacaaa tacatatcct tatattatta gggatacaga
    44221 ggagtttaat atatttatac ccgaaaattt aggatatata gacccagaag ataagtttac
    44281 ttttcaaaat ataaaagaaa atttaaataa attatccatt gtaagaggtt ttagtggagg
    44341 gttcttcttt cattcatatt taaacataga gtatctaaaa aacacaatag aatatttaga
    44401 aaaacagaat atagaattta tgaatttaag agattttaat aattgggtaa aagttgatga
    44461 aatacaaata agaaataatg gagatgaaat tatagtcaac tatgataagg acttagatga
    44521 aataactaaa agtgatacta gatttaaatc catatccaat attagtaaag ttttaatatt
    44581 catagtatct ataagtgttt taa
//
LOCUS       DAMJWU010000023        43637 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMJWU010000023 DAMJWU010000000
VERSION     DAMJWU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.023"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            100..393
                     /locus_tag="PC460_001398"
     CDS             100..393
                     /locus_tag="PC460_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600290.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
     gene            complement(525..1874)
                     /locus_tag="PC460_001399"
     CDS             complement(525..1874)
                     /locus_tag="PC460_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2600291.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKKGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFVGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(1911..3131)
                     /locus_tag="PC460_001400"
     CDS             complement(1911..3131)
                     /locus_tag="PC460_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2600292.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAVVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            complement(3500..5512)
                     /locus_tag="PC460_001401"
     CDS             complement(3500..5512)
                     /locus_tag="PC460_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2600293.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            5983..6633
                     /locus_tag="PC460_001402"
     CDS             5983..6633
                     /locus_tag="PC460_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF2600294.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(6773..8149)
                     /locus_tag="PC460_001403"
     CDS             complement(6773..8149)
                     /locus_tag="PC460_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2600295.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(8165..10078)
                     /locus_tag="PC460_001404"
     CDS             complement(8165..10078)
                     /locus_tag="PC460_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2600296.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            10214..10846
                     /locus_tag="PC460_001405"
     CDS             10214..10846
                     /locus_tag="PC460_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF2600297.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            10985..11524
                     /locus_tag="PC460_001406"
     CDS             10985..11524
                     /locus_tag="PC460_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2600298.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(11700..11870)
                     /locus_tag="PC460_001407"
     CDS             complement(11700..11870)
                     /locus_tag="PC460_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2600299.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            12155..13222
                     /locus_tag="PC460_001408"
     CDS             12155..13222
                     /locus_tag="PC460_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF2600300.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            13392..15293
                     /gene="cooS"
                     /locus_tag="PC460_001409"
     CDS             13392..15293
                     /gene="cooS"
                     /locus_tag="PC460_001409"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2600301.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            15286..15759
                     /locus_tag="PC460_001410"
     CDS             15286..15759
                     /locus_tag="PC460_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2600302.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            15734..16960
                     /locus_tag="PC460_001411"
     CDS             15734..16960
                     /locus_tag="PC460_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2600303.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(17099..18286)
                     /locus_tag="PC460_001412"
     CDS             complement(17099..18286)
                     /locus_tag="PC460_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2600304.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            18559..19383
                     /locus_tag="PC460_001413"
     CDS             18559..19383
                     /locus_tag="PC460_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2600305.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            19512..20777
                     /gene="gluD"
                     /locus_tag="PC460_001414"
     CDS             19512..20777
                     /gene="gluD"
                     /locus_tag="PC460_001414"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF2600306.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            20990..21580
                     /locus_tag="PC460_001415"
     CDS             20990..21580
                     /locus_tag="PC460_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2600307.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            21910..23043
                     /locus_tag="PC460_001416"
     CDS             21910..23043
                     /locus_tag="PC460_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2600308.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGIEYYDSLLND"
     gene            23959..24981
                     /locus_tag="PC460_001417"
     CDS             23959..24981
                     /locus_tag="PC460_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2600309.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            25278..26198
                     /gene="pyrB"
                     /locus_tag="PC460_001418"
     CDS             25278..26198
                     /gene="pyrB"
                     /locus_tag="PC460_001418"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF2600310.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            26237..26929
                     /locus_tag="PC460_001419"
     CDS             26237..26929
                     /locus_tag="PC460_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF2600311.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            26922..27824
                     /locus_tag="PC460_001420"
     CDS             26922..27824
                     /locus_tag="PC460_001420"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2600312.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            27909..28493
                     /locus_tag="PC460_001421"
     CDS             27909..28493
                     /locus_tag="PC460_001421"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF2600313.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            28708..29634
                     /locus_tag="PC460_001422"
     CDS             28708..29634
                     /locus_tag="PC460_001422"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDF2600314.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(29686..30576)
                     /locus_tag="PC460_001423"
     CDS             complement(29686..30576)
                     /locus_tag="PC460_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2600315.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            30764..31795
                     /locus_tag="PC460_001424"
     CDS             30764..31795
                     /locus_tag="PC460_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF2600316.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            32089..32964
                     /locus_tag="PC460_001425"
     CDS             32089..32964
                     /locus_tag="PC460_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDF2600317.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            32982..34472
                     /gene="cls"
                     /locus_tag="PC460_001426"
     CDS             32982..34472
                     /gene="cls"
                     /locus_tag="PC460_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2600318.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            34665..34949
                     /locus_tag="PC460_001427"
     CDS             34665..34949
                     /locus_tag="PC460_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF2600319.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            34982..36610
                     /gene="groL"
                     /locus_tag="PC460_001428"
     CDS             34982..36610
                     /gene="groL"
                     /locus_tag="PC460_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF2600320.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDVGMPGMGGGMPGMM"
     gene            36872..37612
                     /gene="larB"
                     /locus_tag="PC460_001429"
     CDS             36872..37612
                     /gene="larB"
                     /locus_tag="PC460_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF2600321.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            37696..38430
                     /gene="larC"
                     /locus_tag="PC460_001430"
     CDS             37696..38430
                     /gene="larC"
                     /locus_tag="PC460_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF2600322.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            38460..38879
                     /locus_tag="PC460_001431"
     CDS             38460..38879
                     /locus_tag="PC460_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF2600323.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      38939..39040
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            39261..40796
                     /gene="guaA"
                     /locus_tag="PC460_001432"
     CDS             39261..40796
                     /gene="guaA"
                     /locus_tag="PC460_001432"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF2600324.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(40873..41919)
                     /locus_tag="PC460_001433"
     CDS             complement(40873..41919)
                     /locus_tag="PC460_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2600325.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(41996..42211)
                     /locus_tag="PC460_001434"
     CDS             complement(41996..42211)
                     /locus_tag="PC460_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2600326.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            complement(42645..43046)
                     /locus_tag="PC460_001435"
     CDS             complement(42645..43046)
                     /locus_tag="PC460_001435"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600327.1"
                     /translation="MQTLAEIIKEYRSEHNLSLREFSNLCGVSHTYIDKIEKNRDPRN
                     GKAVEPTLDMLEKMSFGLNLTLKELLTKLGKIQPNTQQDDELLKKVNSKEDNEVNELI
                     NRLASLDSDDKNAIKKMIDNAYYKAVNTKKE"
     gene            43211..43423
                     /locus_tag="PC460_001436"
     CDS             43211..43423
                     /locus_tag="PC460_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600328.1"
                     /translation="MKNNLQYIRKESRISQKKFADKIGISRPYLSRIENGKVNPSLEI
                     AHKISLETGKTINEIFFDFTVNHSKL"
BASE COUNT        16642 a         5009 c         7157 g        14829 t
ORIGIN      
        1 aaaatagtta attggatata caatattatt ataaaaaata aatattattt attttagatt
       61 ttagaaattg aaatttcctt aagtgaagga ggtaattaga tgattagtat gattatgatg
      121 cttattggag tattatttat agtgtttggg ataattttaa ctgctgtcga aataaaagga
      181 aaaatgccag gtttttatgg agttgggcaa aatgcagtaa gaaaaaatgc tatagggatt
      241 atagcagcag gtatagcatt aatttttttt agaaatgggt tagatagtat tgattcaatt
      301 cttacctatg gagtattttt agtgatagag ttagtagtaa taggattata ttttcttata
      361 agaataaaat atgataaaga tgggaatctt taaagtataa attaataata ttttgatttt
      421 gatatatgtt tatataaaaa aattgtagat aaattttata aaatagtttt atctacaatt
      481 tttttatatt tattatattt tatgagaagt aatagtataa aatattattg aattttttct
      541 ctcgaaatat ataatttaga atcttctgat ttatttttat gctcgatttc cttatttttt
      601 ttcataaata tatatagagg agctattaac aacattgccc ctaatcctat taaaactcta
      661 ttttgagtta taggttttga aatatcttct gcccaagctt gttgtaacaa ccataagcta
      721 cctaaaatag ctactattgg tataacaggg ccaaatggaa tcttaaaaga ggccttaaca
      781 tctgttcttt ttcttaaaac tattactgat aaacaagttg gtatgtactg agcaaatctt
      841 gcaattacgc tcattactgc aagttgttca aaacttccag ttagcacaag tggtatacaa
      901 caaattaaag atattattat tgctacatat ggagcaccat atttatttgt ttttcctata
      961 aatgcaggaa gagaaccttc ttctgctaga gaagaaccac atttaggagt tactattgat
     1021 gaacctatat ttataccaaa tatagatatt aatgttgcta cagatatgaa aactttacca
     1081 taattgccta caaatacact tgaagcatct gcaattggaa tactgttttc aaatagtcta
     1141 tctccaagta tacccataca gacaatttga attaatatat aaattattgc acatataaat
     1201 attgtggtta ttaatgcgac tggaagattt tttttaggat tttccatttc tccacttgcc
     1261 acaataaatg attcaaagcc ggtaaatgcg taaaatacta atataatggc tggacccata
     1321 ctagagttaa ctacatttcc tgaaggaaca atatttacaa attttataaa aaatattcca
     1381 actattataa aaactattaa tggtactaat ttagctatag ttattacatt attcattatc
     1441 tttgtgcttt taattccaaa taagctattt attgaaagta gtgttacgag tattgcagct
     1501 atataacctt tgtattcagt ggcagattct ggccaaaatg aacctaaagc tgttgcaaat
     1561 cctactgcta gagtagacca tgatataatt ctaataaccc aagacattgt accaacttca
     1621 aatcctataa aatctccaaa agcttttttg gagtatagat atgctcctcc tttcttatca
     1681 aacatgcttc caacttctgc aaaacataaa agtattgata gaactaagag tgtagcaaaa
     1741 atgtatataa acatactgtt ttgacctgct agattataaa ctttacctgg tagtaagaat
     1801 atacctgaac ctattatgct attaatccct aataatatca tactaaatag acctaatttc
     1861 ttagcttttt ccatattaat ttatctccta ttcttttatt aattactgta ctaattaaaa
     1921 tacaaagtat tcattacatg catcataaca cctattggta aacaatcttc atctatatta
     1981 aagctagagg tatgaagagg tgaaattaag cccttttcct catttttaca tcctaaatgg
     2041 aagaatgctc ctttacaatg ttctgtgtaa aaagaaaagt cttcaccacc taaagacggg
     2101 tttggtctca atataaattt ttcctctcca agtaattctt tagtatttga tataactgta
     2161 tctactaatt ctttttcatt tataacagca ggatagtttt catcacttac atgtaaagtc
     2221 ccaacacaac caaatgcaga tgcagtatct ccaacaattt tagctatttt atcaatcata
     2281 aagtttcttg ttttgctatt taaggttctt aaagtgcctt ctaattttac atcttcacat
     2341 attacattgt gtgcatcacc accatatatt ttacctatag tcaaaacaac agcattattt
     2401 ggttctaagt ttctacttat tatagtttgt aatgatgtaa caatttgtga agctgtaact
     2461 attgcatcaa taccgttttc aggatatgca ccatgagctc ttttaccttt gatacttatt
     2521 tttatagtgt ctacactagc atttaatgta tcatattttg tttcgataag gcctgtttct
     2581 atatgtggca taacatgaag tccgaatata taatcggcct tggggttttc taaacaccca
     2641 tcctcaacta aaaattttgc tcctccaaaa ccttcttctg ctggttgaaa taagaatttt
     2701 acatttacat taagtttatc ttttattgaa tgtaaaactt tacatgctcc aagtagcatg
     2761 gttgtatgtg catcatggcc acaagcatgc atttttccag aatgtattga tttataaggt
     2821 atgttgtttt cttcttcaat aggaagagca tccatatctg ctcttataca aatcgtttta
     2881 tctgcacttt cttttaaaat atatgccata attccattgt gttttgtaaa ttccatataa
     2941 tcaattccga tttcttttaa ataagaaatt accttctgtt tagtaagatt ttcttcaaga
     3001 gctaattctg gaattttatg taattctctt cttatgttta taagccatgg ttttatagaa
     3061 ttacaacttt ctattacaaa atcttttcct aattctttgg tttgatattt taatttcaaa
     3121 gcctgttgca tttttattcc tccaattgct ttttattgtt ttattaatat aataagcaat
     3181 tattatgcca taattttcga tggagaattt tgttttttat aagaatattg aataaatata
     3241 atcatttatc tatatgtata gtataaaaaa tgattgcata gtttcaattt tcagtaaaaa
     3301 atattatttt gaagaaaata tattttatat gctagtttat ataaaaaaaa taggaaataa
     3361 aaaaattatc ctattcctat taattcctat tatttatgta caaaataaat tataatttta
     3421 gggtacttga aaaataaaaa aacctattga aggagtactt taatcctgaa tagatttctt
     3481 tgtttgataa attataattt tatagacctt tatattttaa tttattgtaa aaatcttctc
     3541 ctttttgagt tattttactt ccatatcttc ctgaactaca aattataagt tcttcttttt
     3601 ttagatattc aaatattttt ttaatttttc cttctgtaat ttgaaggttc ttatcgagta
     3661 gtgcttttaa aattaatcct ctaccaatac caacatctga ttccttattt aaaattagta
     3721 tctctaatat attcaatata tcattcttac tatattttag ttttaatgat gtatttttgt
     3781 tatctgaaga ccttaagttt ggaggtaaat ctttctctaa tacaatatct tcacacataa
     3841 tgtttaaata ggatgtaaca ttttggagtt ctcttatatt acctttccac atatagtttt
     3901 gtataagttt ttttgcttct ggtgtgatta ctaatttctt attaattaaa tcttccataa
     3961 taattaatat gtcttctttt ctttctctta gaggaggtat atttattgga attgtattta
     4021 atcgataata taaatcctct ctaaattggc tattatcaat catttgttct aaattcttat
     4081 ttgtagcaga tatgattcta acatctatat taattacatt gtgagatcca acaggcatta
     4141 tctgcttttc ttgaagaact ctaagtagtt ttgtttgtaa ttctaaaggc atatcgccta
     4201 tttcatctaa gaatattgtt ccattattag caagttcaaa taatccttgt tttccatctt
     4261 ttagacctcc agtaaaagtt cccttgtcat atccgaatag ctggctttct aataaatttt
     4321 caggaacggc tgcacaattt actgctataa agggttgatt ttttctattt gagttgttat
     4381 gtatagactg agctatgagt tctttaccag ttccactttc acctctgata agtatgctta
     4441 aatcagattt agaaaccttc ttagctaaat ctatacattc aaacatttta ggactatttg
     4501 tttttatatc cttaaatgta tattttgcta tttgtccttt ttctcttaat tttttagtga
     4561 gattttgttc taattttttt atgtatgtta tttcttgtaa actataatat gtgcccattt
     4621 tttcatctct attgtatatg ttttttttat ttacatttat atatttctta ttgaatacga
     4681 caactttatc tagtatctct ttctcactca ataaaacttt taaactatct acaaatatat
     4741 cctcaatgta ttttccatat atatcctcat ttatgtctaa aatatctttt actttactat
     4801 tatatgtatt tatttcacca tcttttgaag taaataatat accatctttt gataaattca
     4861 agattgtatc taattcttct atttttaaaa ataattcctt atatttatct tttataccag
     4921 tatctaaact tattatttct tctgagtatt taactagatt agattgtatt tcctttgaat
     4981 caatttgtaa taaatttatt atttctataa atgtagagat atctatatat ctgtgaccta
     5041 aatcaaatat atctgagata aagctaggta ctttttctgg aactcctgga gttatagcta
     5101 tttttatatt tttatagtta ttatcattca tatatggaat tggtctaata ttagtcactc
     5161 caattttata aaataaactt atagtttcta gagtagtttc atcagaatca tttacgatta
     5221 gaacatttgt accctgtggt aaagatagta gattataaat tttatcttcc ctaaaagttc
     5281 ttcttaccac aatcactttt ttcttatcta gtatattatt tataattttg tctaatcttt
     5341 ctactgacat tactaatatc acatcatcat ttataatatc attatcattt atttcattta
     5401 tgaagtatct attaatattt atagaatctc caaaaactaa tttcaaatta ttttctaaaa
     5461 agtctattaa atcttttcta ctatcattta ccagagctac tgattttttc atttatttct
     5521 ccttatataa taaatagttt ccaacaaaat agttgtataa agcaatctaa ttttgataat
     5581 ttaaatttat caaataaaaa agaaagtgtc tataatgact ttctaaaagt tgcattaaga
     5641 cactttcttt cttatagtta aaaatcttgt caccaaatcc ccctaattgt tgcttaaaac
     5701 ttaaactgta tgtatatttt tcatttctat tcgattaaat tataatatac acccaaaaaa
     5761 atttatttta ataaattcta ttgataatat aattttataa atatagaatt atattaaaat
     5821 aaattatata tgaaaatata tatgtataag tttattctgc gtgagtagaa tggatttgtc
     5881 aagtttaatt tagttgtaat tctatctttt attaaaaata acataaatta aaacatataa
     5941 ataaaatatt ttatacaata tattgtaaag gaggtaactt tgatgaaagc agaaaaaaca
     6001 agtatatatc caattattaa aaataattta aacaaaatat caattgttga atataaaaag
     6061 ggtcaaagat ttgtagcatc agataaagac ttacaagaag tgttctttat aatagaggga
     6121 gttgcattag tggagtgtac tactagagga ggaaataaat ttcttgttga cattgtacct
     6181 gaaaatgaat ttataggcaa aattagttac atatatgaac ataatttgaa atgtgatata
     6241 tttgcaaaga ctaatataaa attatttcga tttgaaaaaa atgtatttga agagttttat
     6301 ctcaaaccag attttattgc actatttcac agaaaatgta caagaagaat atatgaatta
     6361 tataaaacca gaatggtaag agaactattt tcttgcacag aagttattgc ctattgtata
     6421 tcaaataaac aagaaaataa tgtctgcaat ataaaagcta ttcgtaattt cagtgaaatc
     6481 tattcaatta gtagaaaaag tagataccat tctctgctaa agttaactga aaaggagata
     6541 gtaaaaaaaa tgggtaatac atatgaaatt ttaaactatg atgaattaca taaattggca
     6601 tttgaagtaa aagaattttt ggaagatgag taaaaattgt tacttaatca aaataaagta
     6661 taataatttc tgtaaaaaat agaaatagtc tcactgaaaa tcaaaaatac tttagttatt
     6721 ttcaatgaga ctgtttagaa atattgtata cttataatgt atagtataat tattaatcaa
     6781 tttcctttac tatattaata gcccattttt tagataatgc tctaggaaca ccaaatataa
     6841 acttaaccac ttcttcacta taagtgagca aaacgagtac aaatataggt gcacctttaa
     6901 atgcagctat aaaagttaga ggaatagaag ctaaccacat acatcctaat tctaaaaata
     6961 gagcatattt agtatctcct ccacttctta aaataccaat tataaccaat gtattaaaag
     7021 atttaagagc cattagtatt cccattataa agaatatctt agttatatca gaagctaagc
     7081 tatcagaaac actaaacatt tttaataata atggtatatt taaaattaaa ataattccaa
     7141 gtattaaacc agctgaaaat acaagtatag aaaacttttt agcatattca atggctcttt
     7201 taatatggtc agctccaagt tcatttccaa gcataataga tgcaccagat gcaatacata
     7261 cagctgtcat tatgaagaaa ttacctgtac tggttgctat ctggctagct gctatagcag
     7321 aagtaccagc tcttgcatat gcaacagaat acaggacagt tccaattgcc catacagagt
     7381 catttacaaa tataggaaag ctttttgatg aaaaagattt tataaaaatt ttatcaatag
     7441 cttttagatt ttttagacca aactttaata tatagtcttt tttataaagg taaacataag
     7501 taatcattaa aattaattca catattctag caataacagt agctagagca gcacctttta
     7561 cacccaatgc aggaagaccg aagttaccaa atataaatcc ataatttaaa ataacatttg
     7621 ttacaagtgc aaatgcacta caaatcatac ctaacttagg atttctaaca cctcttgaac
     7681 cagtactaaa tacagtactt acagcaatta atggataact aaatactgca atactaaagt
     7741 aatctataca aagttttacc acctcagaat catacgaaaa tatgtgtata attggctttg
     7801 gtgttagtaa agcaggaata acaaatacaa aacttaaaac aattgccaat acacaagtaa
     7861 gacctgtgac ttttcttata ttactcacat ccttctttcc ataaaactgt gaaataaaaa
     7921 ctccagcacc tcctgtgata ccagataagg acatattaaa aagaaagaaa aattggtttg
     7981 caactccaac tgaagctaca gatgtttccc ccaaactact tatcataatt gtatctatta
     8041 catttacaga agtagaaatt aagttctgta tgataatagg tatacataaa gatagaagta
     8101 ttttataaaa tcttttattt tcgtctttta aagaaaatag ttcttgcaaa taatcctctc
     8161 ctttttacat aaattcttcc cactcatcat atagagaggc aaggttattt tctaaattaa
     8221 ttttttcttg gctaacttct ttagctttat ctggattaga gtatacttct tcttgacata
     8281 aaagtatatc taatttttca atttcaatct ctaacttttc aatttctttt tctatatttt
     8341 gtctttttat tctatttttc ttttctattt ctctttgttc tctctctttt cttttttcat
     8401 cttttatttg agttctagtt ttttcttctt tttcttctat aatactcatc tcttggattt
     8461 gccttttttt atctatataa taatcataat ttcccagata ttcagtaatt ccattttcat
     8521 ctaaaactaa aattttatca acaacagtat ttagaaagta tctatcatgt gatattgtaa
     8581 atatagtccc atcataatta gttaaggcct cttcaaggac ttccttagaa tcaatatcta
     8641 ggtgattagt aggctcatct aataacaata agtttgcatt tgatagtata agtttaagta
     8701 tggcaactct agctctttct ccaccactca atgtagaaat cttcttaaaa acttcttcat
     8761 ctacaaataa aaatgcccct aacatatttc tcaaagtagt ttgagtaaga tgagtattat
     8821 tttcccaaat ctcatcaatt attgtgttat ctaaattaag agttttttgt tcctgatgga
     8881 agtaggaaac atgtacatta gttccaaatt taatattgcc actaagaggt tgtagttcat
     8941 tagttattat tttaaataat gtagatttgc caacaccatt tgcacctata agtgcaactt
     9001 tttcccctct atatatatct aaatccaagt ctttaaaaag tattctttct ccataaccca
     9061 tagatacatc tctaatttga agaacatcat taccactctg gacagaaggc gtaaattgta
     9121 tacgagcttt tttcctataa gcttctggtt tatctaaaac atctactttg tctaatgctt
     9181 tttctttact tcttgcacgc tttaggtgtt tttctctacc ataagctttt agtctttcaa
     9241 ttgattcttc ttgctttttt atttcttttt gttggtcttc aaatttctta agctctagtt
     9301 ctttttctat tttagataac tcaacaaatt tagagtagtt accattatat actttaagct
     9361 ttttattgtg aacttcaaaa attctattta cggattggtc taagaaatat ctatcatgag
     9421 atattaacat gacagtccct ttatattgtt ttaaaaatac ttctaaccat tctattgctt
     9481 cagagtctaa gtggttagta ggttcatcaa gtaacaatag agttggtttc ttaagaagta
     9541 attttcctaa aagaactcta gttttttcac caccagaaag tatgcttatt ggcttatcca
     9601 tatcattgtc actaaatcct aatcccttta gaactccttt ggcttcagat ttatatccat
     9661 atccatttaa atctgaaaat aattctagtt tatgtgaata ttcatccatg attttttcta
     9721 taattggaga gtttgttttt gtactttctt cagatatttt aacctccaat tctcttaaat
     9781 aggattccat ttctatcaaa tttttaaaaa cttctagtac ttcttccaaa atagtattat
     9841 ccgaataaaa attggtattt tgttctaaat atccgatttc acaatcttta gaagtgtaaa
     9901 tatctccact atcatatcca taaattcctg aaattatttt aaataaagta gtttttccag
     9961 taccattaac accgattata ccaattttat cgccttcatt tactgtaaaa ctaatatttt
    10021 ctaatataga atctatacca aaacttttat ttaagttatt acatgacaaa acaatcataa
    10081 atttctacct ccttgaacta tctaaaatta taatgttttg atataatata atcaaggtta
    10141 tatgtattag ttttgaaaaa gaaatatttt attgtgaatc aaacaaaaaa gaggacactt
    10201 ttaggaggtg aagatgttgg gaaataaaaa tatatcaatg gcagttataa gaaggctccc
    10261 aaaatatcat agatatcttg gagacttatt agatagggat atacaaagaa tatcttctaa
    10321 agaattgagt gatataatag ggtttaccgc ttctcaaata agacaagatt taaacaactt
    10381 tggtggattt ggacaacaag gatatggtta taatgtagaa gctcttcata ctgagatagg
    10441 taaaattctt gggttggatc gaccatacaa cgcagttctt gtaggagcag gtaacttagg
    10501 acaagctata gccaattatg caggatttag aaaagctgga ttcgagataa aagctttatt
    10561 tgatgcaaat cctagaatga taggtttaaa gataagagag tttgaagtat tagattcaga
    10621 tactttagaa gactttataa aaaacaataa tatagatatt gctgtattat gtatacctaa
    10681 aaatggagca caagaagtta ttaatagagt tgtaaaagct ggaatcaaag gtgtatggaa
    10741 ttttgcacct ttagatttag aagttccgaa aggtgttata gttgaaaatg taaacttaac
    10801 agaaagttta tttaccttat cgtatttaat gaaagaagga aagtagacaa cttaaacata
    10861 ttaataatga gcagttgtaa agagtattga gaaagtcaga agatttaata ttaaatcctc
    10921 tggctttttg aaatattagg ataaaaattt aaataatgta tggaaataaa caatattaag
    10981 gagagtggtt tttgtggagg aattatggaa aaaggcaatt gaatttcatg gtcatgaatg
    11041 tccagggtta gctataggat ttaaagcagc acttgcagca aaaaaatatt taggatgtaa
    11101 acagtcagga gatgaggaaa ttgtatgtat ttctgaaaat gatgcttgtg gaatagatgg
    11161 tatacaagta gtgctaagtt gcacaatggg taagggaaat ttactactta ggatgactgg
    11221 aaaatcagta tatcatttct ttaatagaga aaatggaaaa tctataagga tatatgtaaa
    11281 gtcaaaacca tttgagatgg atagagaagg atacaaaaga tatttattag aaagtgattt
    11341 tgaagaaata tttgagatta cagatactaa gttaagactt cctgaaaaag caagaatatt
    11401 taaatccctt aaatgtgaag tgtgtggtga atttgcatct gagcaatata taaggattga
    11461 agaaggaaag aaagtatgtc ttgattgttt tgataaatat gaaagatttt atgaagagta
    11521 ttaaaattat aaattagatt aaagttaaaa gtattagata tgtgctaaca gtattttaag
    11581 atgaattagg tagcaacata ataaagtata taaataatat taattgaatc atacataatt
    11641 aaaaagaggc tgattagtat ttataaaata aaatcagtct ctttatgaat aacgaattat
    11701 tattctggtt gaggtgcatc tacaggacaa gctcctgcac aagaaccaca gtctatacaa
    11761 gaaccagcat ctataacata tgcgtcgtct ccagctgata tacagctaac agggcattca
    11821 gcctcacaag caccacagct tatacaagcg tcagtaattt tatatgccat tttgaaatcc
    11881 tcccttttat atgtattgta attcaataac attataacaa atattattga atataacaat
    11941 taaattaatt ctacaacaaa aactttatta tgatattctt gttttaaagt tttacaataa
    12001 agtttaatct gcttatattt ttatttattt taaagaagtt tattttaaaa ataaaattaa
    12061 atttaaaaag ttatgtattg attatattta aaaatgttat actctaaaaa agtatatttt
    12121 atttaaaaat ttattaatac aaaggagatt tactatgaaa aataaattag tttacatatg
    12181 tattatgagt ctattgctat ttgtagtagg atgttctaat aatccaaata aggaaagtac
    12241 cattaaaaaa gactctaaga ataaccaaag ccaagttaaa gaagaaaaaa gagaacctgt
    12301 gaaaaaagat ataactataa gtttcgctgg tgatgtaact atggggaact ataaaggttc
    12361 atcatattat ggcagttttg accatgaatt taaaagacaa ggagaagatt acaattattt
    12421 tcttaaaaat gtaaaacctg tatttgaaaa agatgactta acagtggtta atttagaagg
    12481 tccacttact acagcgtcaa atgcaaaaga aaaaaaattt gcatttaaag gaaagcctgg
    12541 atatgtaaat attttaaaat ctggaagtgt agaggcagta agtattgcaa ataatcattc
    12601 agaagattat tttgaagaag gaatgaaaga tacacagttt atattggacg agaataaaat
    12661 aaattatttt ggattaggga aagatgctgt tgtagatgtc aaaggaatta aagtgggtct
    12721 attagggtat aatggattat caactgaata taatgaaaaa aatttaaaac aaatggaaga
    12781 tgatattaag tccttaaaaa agaaatcgga tgttgttgta gtgtactttc attgggggat
    12841 agaattagat tattatccag ataaaaaaca aaaagatttt gctcattatg ctatagacaa
    12901 gggagcagat ttagttatgg ggtctcaccc acatgtgatt caaggaatag aaaagtataa
    12961 aaataagtat atagcttata gtctagggaa tttttgcttt ggaggaaata agaatccttc
    13021 tgatacagat agttatatat atcaacaaac ctttactttt tcagataata aattaacttc
    13081 aattaaagag ccaaatataa taccaacttc aataacatct agtaactcca gaaataatta
    13141 tcaaccaaaa atattggatg gaagtgagaa ggatagagtt ttaaataaac ttgaaaaaat
    13201 aagcaaagat ttaaataaat aaaatgtaaa aaaaaataaa tatatatatt tattttaata
    13261 atttatattt tgtttagggt atggtaaatc gtggtattaa ataagtagat tttaatagtt
    13321 aaattatagt caattataat aaagctattt aatctacttt tttaatataa aaatataagg
    13381 gggaatttgt aatggcttgt aacacttgta aaatgtgcga aagtgcagac aagaaattag
    13441 aaagttttgt agcttcaaag gatgttgaaa cagcatttca tagaacagaa gatcagaaag
    13501 ttaagtgtgg ttttggactt cagggggttt gttgtagact ctgttcaaat ggaccatgta
    13561 gagtaactcc taaatcacca agaggtatat gtggagcaga tgcagacact atagtagcaa
    13621 gaaacttttt aagagcagta gcatcaggag cagcttgtta tttacatgtg gtagaaaata
    13681 cagctaagaa tttaaaaaat gttggaataa ctaaaggtgt tgttaaagga gaaaaaacat
    13741 taaatcaatt agcagagatg tttggaatag aaagtgatga aaagtatgat aaatgtataa
    13801 aggttgcaga caaagttatc aacgatttgt ataaatcaag agatgataaa atggaattag
    13861 tagaaaaaat agcttatgca cctagagtga aaaagtggaa agagttaggt ataatgcctg
    13921 gtggtgcaaa gtcagaagtt tttgatgcaa tagtaaaatc atcaacaaat ttaaacagtg
    13981 acccagtaga catgctagtt aattgtttga acttaggtat atcaacagga ttatatggtc
    14041 ttacactaac taatctttta aatgatgtta tgttgggaga accagttata agaatggctc
    14101 ctgtaggatt taatgtaata gacccagact acataaatat aatgataaca ggtcatcaac
    14161 attcaacatt tgcaaatttc caagatagat taaaagatga agatgttata aaattagctc
    14221 aatctgttgg cgcaaaagga tttaagttag taggatgtac atgtgttggt caagatttac
    14281 aattaagagg agaacattat caagaagtat ttgctggaca tgcaggtaac aattttacaa
    14341 gtgaggctgt tttatcaact ggagcaatag atatagttct ttcagaattt aactgtacta
    14401 taccagggct tgaaccaata gcagataaat ataaagtaaa aatggtttgt ttagatgatg
    14461 ttgctaaaaa agcgaatgca gaatgcatag gtttagacag aagtaaatta gatgaactta
    14521 gtaacacttt aatagaaaaa gcattggagt cttataagga gagaagagga agtattgaaa
    14581 tagatatacc aaaagaccat ggatttgaac aatctttgac tggtgtaagt gaaaagaact
    14641 tgaaagcgtt tttaggagat tcatggaaac ctcttattaa tttaattgca gaaggaaaaa
    14701 ttaaaggtgt tgcagctgtt gtaggatgtt caaatatgac agcaggagga catgatgtaa
    14761 atactgttga acttactaaa gaattgatta aaaaagatat aatagtacta tctgcaggat
    14821 gttctactgg aggattagaa aatgttggtc ttatgtcacc tggagcagaa gaattggcag
    14881 gagaaaactt aaaagaagta tgtaagactt taggaatacc accagtatta aactttggac
    14941 catgtctagc tataggaaga ctagaaatag tagcaacaga attagcagct gaacttggaa
    15001 ttgacctacc acaattacca ttagtgcttt cagctccaca atggttggaa gaacaagcat
    15061 tagctgatgg agcttttggt cttgcccttg gcttacctct tcatttagct ttacctccat
    15121 ttataacagg aggaaaattg gtaacagaag tattaacaga aaaattaaaa gacttaacag
    15181 gaggacatgt tatagtgaat ccagacccta aatcttcagc taatcaatta gaagaaataa
    15241 taatagatag aagaaaaaat ttagggttaa aggatgtgga atgcaatgca tagaattttt
    15301 ataaataaag atttatgtac aggatgtaag agctgtgtac tagcatgtat gctaaaacac
    15361 aataaagatt atgacatgta cactcttgat ttagaaaata tagataatga tagtagaggg
    15421 catatagaat tagatagtag acataataat ccagttccaa ttctttgtag acactgtgat
    15481 gaacctgaat gtgtattagc atgtatgagt ggtgcaatgc ataaagatag tgaaagtgga
    15541 atcgtttcat atgatgaaga gaaatgtggt tcatgttata tgtgtgtaat gtcttgtcca
    15601 tatggattat taaagccaga tgatagaagc aagcagaaca tacttaaatg tgatttatgt
    15661 aaagatgagg agtatcctag atgtgtagct aattgcccaa gtggagcaat tgaattacag
    15721 aaggaggaaa atgatgaatt atgtagtgtt aggggctagt gcagctggta taaatgcagt
    15781 aaaaacatta agagaattag ataaagattc aaatatagta gtaatttcta aagatgaaaa
    15841 cgtatattca agatgtatgt tgcatcacgt tatatctgaa cacagaactt taaaacaaat
    15901 aaattttgtt gatgaagatt ttatggagca aaataatgta aaatggattg caggtaagac
    15961 tgttaaaggt atagacataa ataaaaaggt agttcagaca gaagacatta ctgtaaatta
    16021 tgataaactt ttaatagcta caggtgcatc ttctgctatt cctcctataa aaaatttaag
    16081 agatggaaat tttgtatatt ctgtgagaaa catagatgat atttataaaa taaaagaaaa
    16141 agcacaaaat tctaaaaatg tagttataat aggtgctggt ttagttggta tagatgcatt
    16201 agtgggatta tttaaatatg agcagttaaa tatttctgta gcttttatgg aaaagtacat
    16261 tttagataga caattggatg aatatacagc atcagtgtat gaaaataagt tcaaagaaaa
    16321 aggtgtaaaa ttttacccaa gtgcatcaat acaagaaatt gtattggata acagtaaaaa
    16381 tgttacaggt gttgcatttt caaatggtga agttttagat gcagacatgg taattgtagc
    16441 tacaggggtt aaaccaaatg ctgacttttt agatggaaca ggaatagaat atgatagagg
    16501 tataataata gatgatatgt gccaaacaac acaaaaagac atatatgcag caggagatgt
    16561 tgtaggtaaa aatgctattt ggcctctggc agtaaagcaa ggaattgtag ctgcctataa
    16621 tatggttgga aaagataaga agatagaaga tgaatttgct tttaaaaata gtatgaattt
    16681 catggatata ccaactattt ctataggaat gaatacacct gtagatgata gctataaagt
    16741 attaaccaga catggaatta atgattataa aaagtttgtt tttaaagata atgtaatcta
    16801 tggagcagtt atacaaggag atatttcata tgtaggtgta ttaacttatt taataaaaaa
    16861 taaagttgaa atatatgatt tagaaaatag gatatttgat atatgttatg cagacttttt
    16921 taatataaaa gaaaatggag aattttgtta tagtgtatag tatatacgtg gagtaaggtt
    16981 tataaataca gtaaatcaat aaatagagtt attctaaaat aagaaaaatg acaaatctta
    17041 tttagaaata gctctatttt ttgtaattaa atttaaatta tattcaattt gtaaaatatt
    17101 attttgaaca attatacata tactctctag ttagaggtaa atcatttttt atacctttag
    17161 tgaaaagtat ttgatgtata tctataatac cagtgttgaa agcagcagca caagaacaaa
    17221 ggtataaatc ccacattctt ataaacttat catcaaacat ttctttaatc tttggaagat
    17281 tctcagagaa atttttttgc cagtatagta aggtttttac ataatgtaat cttaaacttt
    17341 ctacgtctat agtatgatat ttgtaatcac tacaaatatg tatcatttct cttaggctag
    17401 gaataactcc tcctgggaaa atatattttt ttatccaagg gtctccataa gattcctgca
    17461 atccacttat atagtgaagt aaaaacaatc caccatcttt taaaacagca tttacgtttt
    17521 taatgaaaag ttcatagttt tctctaccaa catgttcaag catcccaacg cttacaactc
    17581 tatcaaacat aaggtctgat ttttttaatt ctctatagtc cataagtctt acctcaagat
    17641 agtcttgaag gttctcttct ttaattcttg cgttaaattc cttaaattgc tcttcgctaa
    17701 gtgtaattcc aacgccgtga attttatatt tttttgcagc ttctattagt aaaaatcccc
    17761 aaccacaacc aatatcgaga aggctcatac cttcctctaa atttagttta gttaatatgt
    17821 ggtgaacttt attgacttga gcatcataaa gagtatcatt atcatttcta aagtatgcac
    17881 aagaataact aagtgtctta tctaaccata agctataaaa atcgttgcca atgtcatagt
    17941 gggcatgtat ttctttcttt tgatgtctca ttgaatttga tgagtgcaat agatgtttta
    18001 cagctgaatt atcaatattg aatttgtcta tttgttttaa aagtagattt aaagttaaga
    18061 ataaatctcc ctcaacttca atgtctctat ttatgtaggc ttctccaagt gctaaagatg
    18121 tactttttaa tagttcagac ttgctaatat cattatttac tataactttg aactgagggg
    18181 agccctctcc aattagaaat tcttcctcat ctttaagcct aactagaaat ggtgtttcac
    18241 caaatttctc taaatagtct tttaaaaaat tttttgtaat attcatatta ccagctcctt
    18301 ctaatgtaaa tccagtaagt ttccctgctt aaaatatatt atagtccttt ttttaaaaaa
    18361 gtaaaatttt ttcaataaat tttttattta aaggaaattt attttttgta aagaaatttc
    18421 atgttgtagt atattttttg cttataaatt ttggtactgg atataatatt aaactgttaa
    18481 tgatataata aatttaatct ataaagtaaa taaaattttg taataaatag ttaaagctat
    18541 tttataagga gatatatgat ggaagtatat gctttggttg ggtcaagtgg cacagggaag
    18601 agctttaagg ctttagagtt cgcatatgaa aatgatatag aatatataat tgatgatggg
    18661 attttaatat acaaaaacaa ggttttagct gggatatcgg caaaacaagc aaatactact
    18721 atagaagcag ttaaaagggc tattttttat aatctggagc atagacaaga agttagagaa
    18781 gcaattaaaa aagaaaatat aagaaaaatt cttattattg ggacatcaaa aaaaatggtt
    18841 aatcaaattg tagagagatt gtcaattgga agggtttgca agtttataaa tatacaagat
    18901 ataagtacta aatatgaaat agaaatagca aaacaagcta gaaaagaagg aaatcatgta
    18961 attcctgttc cagcagttga gataaaaagt atggcaagtg gtcttagtat aaattctttg
    19021 aagagacttt ttagaaaagg aaataatagg aatatggtag ttttagaaaa aacaattata
    19081 agaccaactt ttagttatat aggaaagttt tatattagtg ctgatgtaat taaacagatt
    19141 attgaatatg aagtatacaa ctttggaagc attgatagaa taaataaaat caatatagag
    19201 aattgcaata attttatgaa tatttttgta agtataaata tcaatgattt agaaactata
    19261 aaaaaaattg aagacataca acagacaata aaaaagtcta ttgagaaaat gactttagtt
    19321 aatgtacaaa aaatagatat taacattcat aaaattaaaa aaataaactt cctccaatgt
    19381 taaaaaataa actgagaaaa tgatatacta atttgcaata aagtgtgcta taattaaact
    19441 gtaaatttag gaaatggttt tttctaaatg gagtatacat aattataacg tatttataag
    19501 ggggctttca aatgtcagga aaagatgtaa atgtcttcga gatggcgcaa tctcaagtaa
    19561 aaaatgcatg tgataaatta ggtatggaac cagcagttta tgaattatta aaagaaccta
    19621 tgagagttat agaggtttca attccagtaa aaatggatga tggttctata aaaactttta
    19681 aaggatttag atcacaacat aatgatgcag tagggccaac aaaaggtgga ataagattcc
    19741 atcaaaatgt ttcaagagac gaagtaaaag ctttatctat atggatgact ttcaagtgtt
    19801 ctgtaacagg tataccatat ggtggaggta aaggtggaat aatagtagat ccatcaactt
    19861 tatctcaagg tgaattagaa agattaagta gaggatacat agatggaata tataaattaa
    19921 taggtgagaa agttgacgtt cctgcaccag acgtaaatac taatggtcaa ataatgtctt
    19981 ggatggttga tgagtacaat aaattaactg gacaaagttc tataggtgtt ataactggta
    20041 agccagttga atttggtggt tctttaggaa gaacagctgc tactggattt ggtgttgctg
    20101 ttactgcaag agaagctgct gctaaattag gaatagatat gaaaaaagct aaaatagctg
    20161 ttcaaggtat aggaaacgtt ggttcttata cagttcttaa ctgtgaaaaa cttggtggta
    20221 ctgttgtagc tatggctgaa tggtgtaaat cagaaggttc ttatgctata tacaatgaaa
    20281 atggtttaga tggtcaagct atgttagatt atatgaaaga acatggtaac ttattaaact
    20341 tcccaggagc taagagaata tctttagaag agttctgggc ttcagatgtt gatatagtaa
    20401 taccagctgc attagaaaac tctataacta aagaagttgc tgaatctata aaagctaaat
    20461 tagtttgtga ggctgctaat ggaccaacta ctccagaggc tgatgaagta tttgctgaaa
    20521 gaggaatagt tcttactcca gatatattaa ctaatgctgg tggagttaca gtttcttact
    20581 tcgagtgggt acaaaactta tatggatact actggtcaga agaagaagta gagcaaaaag
    20641 aagaaatagc tatggttaaa gcatttgaat ctatttggaa aatcaaagaa gagtacaatg
    20701 ttacaatgag agaagctgct tacatgcatt caattaagaa agttgctgaa gctatgaaat
    20761 taagaggatg gtactaagat ttactcttaa taaataatag gatataatta tttaagatat
    20821 aaaggggctg tacatagtac agcccttaaa ttatgtggct tacaatgaag taagctattt
    20881 ttgttttaac ttataaataa cttgataaat tatgtaattt agaatgtatt ttttaccacc
    20941 acatgattac aaattagagg agttagattg aatttttaga catgaactta tgcattttaa
    21001 aagcaaagac cttaatttaa agtatctagt attattttta aaaatagtat attggtttaa
    21061 tccatttata tgtataatgg aaaaatatat ggatttggat tgtgaacttt attgtgatga
    21121 gagagtttta aaaaattgta ctgtacaaca aaagcaagaa tatgctctaa ctattgtaaa
    21181 ttcaatgaaa agaagtacaa atttttcaaa taaatttgta gctggattaa ataaacaatc
    21241 tgatattaga aaaagagtaa taaacatgtt cagtgaaaaa cataaaaatg gtatcttaag
    21301 ggctttgatt ataagcttgt tttcttctat tacatttttt aaattcgatt caactagtat
    21361 tattgaagag aatgatttct tagagaggtc aagatatacg tttgaaggaa ggcaagaaga
    21421 catgtttgta acaatagatt ttatatatgc agatgtacct ataaaactta gaaaagagca
    21481 tgaagaaaac tgtagagtca atggaatagt accaaaagat tcagattcaa tagaaattat
    21541 tcctagattt tataaagatt gtatggattt aaaaaaataa gttagactta atattgatgt
    21601 tgatattatg aattttttat atttaagcaa ttgaaaattt tattatagat tcaattttat
    21661 atgtgtttaa gtatgaaatt tatagatagt tgaaataatt aaatatgatt gatgcatagg
    21721 aatcattact aagctataat taattatagg ggagtttaaa taaattatga gttacacaat
    21781 cataaaatga taaaaatagt tatctgttac aaaaaatgta tctttttaat taataaagat
    21841 aatatgaaaa cttaaaaggc aaataaactt aaatataaaa tactcttaaa taagatagga
    21901 gaaaaatgta tgtctaactt tattctaaat gggattttta gaacagttat agtaagtagt
    21961 ctaagcattc ttctgatatt gattttcaag aaaaatgtat ttaaaagatt tagcaaaaaa
    22021 tttaattatt atatatggat gatagttgtt attaaattat ttttaccatt tacatattat
    22081 acttttacaa taaatatctt gagaagtaaa aaacacatta atattaataa cataaattta
    22141 gagggattta ataatgtttc aactataagt aataatatta tattgtacac ttggattata
    22201 actgtagtag tgtatttaat ttacactata tttaaatata taaaacttaa aaatttaata
    22261 aatgacttat cttatgatgt tgatgataga gagattgtaa atctttataa aaatatatta
    22321 gaagaattta atataacaaa agatataaaa ctcaaatact cttatgaagt tgaaacacca
    22381 gcatttttta attcatgtgt acttttgcca ccacatgagt ataaattgaa agagttagac
    22441 tggattttta gacatgagct tatgcatttt aagagtaggg acctttattt aaaatatgta
    22501 atcctatttt taaaaacagt atattggttt aatccattta tatatataat ggataaacat
    22561 atagatttag attgtgaact ttattgtgat gaaagagttt taaaaaatcg aaattcagag
    22621 gaaaaaaagg attatgcttt aacaattcta aatgcaatga gaaaaggttc aaatacttca
    22681 aataaattta tagctggact tcataagcaa tctgatatta aaaagagagt tatgaatatg
    22741 tttaatgaaa agtacaagaa tggtatatta atggcattaa ctttatgttt attatcttct
    22801 attacttttt tgaaggttaa ttcaactagt attgttaatc ttaatgtatt acctaaagga
    22861 tttgaagact tacgaactgt tagcagtata cgtttttcag ttataggatt tacatatgca
    22921 gatgctccta aaaatatgag ggaagaatat aaaataaatt gtgaatcatt aggtttgata
    22981 cctaaagact ctgataaaat agaagttggt atagaatatt atgacagtct attgaatgat
    23041 taaataaata ttattttaac agatattaat tataaagttt tgaatagtaa tttgttgaga
    23101 aataaatata tccttataaa aatagatact tatataccaa gaataaaaaa tagtcaagat
    23161 agtacacaag gatatactta taaagaactt aaataagtga aataaattat taattataag
    23221 gtttataatt ttaaaaaatt agataaattc aggtgtaaaa tactataaaa cacaattcgg
    23281 aaaagtgtat gttgaacttc atttaaaatg gaatttcaaa gttgctatag taagtagttt
    23341 aagcattctt cttatattaa tctttgagaa taatgtattt aaaaaagtta gtaaaaaatt
    23401 taactattgt atattaatga tagttgctat tgatatatta tctaaaaaaa ttaagtatga
    23461 aaagattact gctaggacaa tcttttcata cttaattttt aatatttata ttatccaaaa
    23521 ttttcatctt tttaacttag aatttgagaa tcactattag gaaataggct ttgatttatg
    23581 tagggttaaa aatttcacag aatatctaac tttatactaa atccatcaaa aaatatacgc
    23641 catacaaaag cgcataaaaa aacacataaa acaatattta tgcgcacttt aacctcgaat
    23701 ggtacaaaat tctttacttt tacagaccca tattatataa ttaagtaaat aatgtatatt
    23761 taggtgacat attggataag ctaatttatt tattttatta atataataat actataaaag
    23821 ttatataaag tggtttacaa aaaagttaca gatagagctt ttattaatgt attgtgtatt
    23881 agtattttat ccttaggtac acttcaccta caaaatagaa tcataagata attatgggaa
    23941 aattttagga ggtttatcgt gaagaaaaag atattaatac cagtatttgc atcagttatg
    24001 gcattatcag taagttcaat tgtaaatgca gatgaagtaa atgattcatc tcaaaataag
    24061 gatgataaaa caaatactga attaaattta ggtgaatata aagaagtaaa atataaagtt
    24121 gcaaaaataa aagatggagt agctataaag ataagagaag aaggtcaagt tcaaaacata
    24181 gcttattctg gagatgaatt tacggtttta ggaactcaag gagaatgggt taaggttaaa
    24241 gttgaagatg gcgaaggttg gttagctact agatatgtag atatatctga aggtgtagga
    24301 tatactaacg ctgataaagt aaacttaaga aaagataaga gtgaaagttc agaagtaatt
    24361 gaagagctgg aaaaaggaag ttctttattg gttttggaag ataatggaga ttggttaaaa
    24421 gttaaagatg gggaaacaga aggatatgta aaatcatctt atatatctga taaagctcct
    24481 gtaatagaag agccacaagt aaacccaagt attgaccaaa atgtagattc tacaaataat
    24541 aatcaacaaa acaatgcaaa tactaatcaa caaaataata attcaaatgt gccaacagct
    24601 aactctaatg cagttcaagc tgtacttaac ttggcatatt ctaagcaagg ttgtccatat
    24661 gtttggggag cagaaggacc aaacactttt gactgttctg gatttacaca atatgtgtat
    24721 agaaatgcag taggaaagaa tataccaaga acatctaaag cacaatctaa atatggacaa
    24781 actgtaagta aagcaaattt acaaccagga gatttagtat tcttcacaac taatggttct
    24841 ggttctgtta gtcatgttgg aatatatgta ggtgggggta atatgataca ctcaccatca
    24901 acaggaaaaa cagttagtgt aactagtata aattcatcat actatacagc aagatttgtt
    24961 acagcaaaaa gaatattata atcggtttgt aatatgacaa attgataaat acataaatat
    25021 atataaacgc tctatattat atttaattta atcatggaaa caaaataatg attatatttt
    25081 aaatgtacta tagagcgttt ttgtatcact ttaaatctaa tcaagattca agttaatatg
    25141 actgttaaat aggagccttt tcatatgatg attatttgtg caatcccttg acaggatagg
    25201 gtaaaggtgg taagctacta acagtacaaa aatactttta attttagttc agagaggcta
    25261 aaaaaggagg acgaaagatg ttaaaatcaa gaaatttaat ccaaccagaa gacttttcaa
    25321 tagaagaaat agatgaaata ttagaattag ctcaaaagat aattgacaat ccgtcaaaat
    25381 attctcgtat atgtgaagga aaactattag ctacattatt ttatgaacca tcaacaagaa
    25441 caagattaag ctttgaatct gctatgaaca gattaggagg acgtgtagta ggcttttcag
    25501 agccaaattc atcctctgca tcaaaaggtg aaactctagg agatacaatg aggatagtat
    25561 ctggttatgt tgatataata gctatgagac atcctcaatc aggtgcagct tcagaagcag
    25621 ctagatatac ggaagtgcca tttataaatg caggagatgg caaaaaccag catccaactc
    25681 aaacacttac agacttactt actataaaat cattaaaagg aactttagaa agccatacaa
    25741 taggattatg tggtgattta aaatatggaa gaactgtgca ttctttggtt aaggctatgg
    25801 caagatataa aaatacaaag tttgtattta tagcacctga agagttaaaa atgccagatt
    25861 atataaaaga agctataaag ggacatgcat attatgaaac taataatttg gatgatgtta
    25921 taggaagctt ggatgtttta tatatgacta gagttcaaca agaaagattt gaagataaat
    25981 cagagtatga aagacttaaa aactattata ttttaaataa agctaaatta gaaaaggcgt
    26041 ctaaagatat gttagtaatg catccattac caagagtaaa tgagatagac atagatgtag
    26101 atagtgatga tagagctgta tattttaaac aagctaaata tggaatgtat gttagaatgg
    26161 ctttaataat aaaactttta ggtataaatg aagactaata gttatcatat aatagtaaaa
    26221 caaaggagat tttgggatgt ataaaattct ggaaaatata tatataggcg aagatatgta
    26281 tagaatgaaa gtaaaaggta attttgaagg taaaatggga caattctata tgttaagagc
    26341 atgggatact tatccagtac tttcaagacc tataagtata catgatatag atgaagaagg
    26401 gataactttt ctttataaag tagtaggaga aggaactcaa attttgagta acctaaaggt
    26461 aaatgatact ataaaattgg aaggccctta tggtaatgga tatgctaagg ttgatggaaa
    26521 ggtagctcta gtaggaggag gaatcggtgt agctccacta tatttggttg ctaaaaatat
    26581 aaaaaattgt gatgcatatc ttggctttag agaagatgtt atattagaag atgaatataa
    26641 acaagtatgt aataaagtat atacaacagt aggaaatact tttgtaacag atataattga
    26701 tgttgagaaa tatgattata tattaacatg tggtccaact ccaatgatgg aaaaattagt
    26761 taagatggta gaaggtacta aaacgagaat tatggtatct cttgaaaacc atatggcttg
    26821 tggagtaggt gcctgtttag tatgtacgtg taaaactaat ggaggaaata aaaagacttg
    26881 taaagatggt ccagtgttct ggggagagga cgtgattttt aatgggtaat ttaagtgtga
    26941 aatttggaag tgtagaattt aaaaatccag taataatggc gtctggaact tttggatttg
    27001 gaaaagaata taatgagatt tatgatatac aaaaacttgg tggtataagt agcaagggtc
    27061 ttactttaaa taaaaaacct ggtaacaatg gtatgagagt tcatgagaca ccatccggta
    27121 tgatgaatag tgttgggctt gaaaaccctg gagttcaagg atttatagac tatgaattac
    27181 ctttttttag taaactagac ttagtcagaa tagctaatgt tggaggtggg acattagaag
    27241 actacttatt aggggttcaa atgttaaatg acaagcctat agacattatt gaattaaata
    27301 tatcttgccc aaatgtcaaa gctggtggaa tggcttttgg tataaaaaat gaagtggcaa
    27361 gagaagttgt tcgtgaggta agaaacataa cgaaactgcc tttagtaata aaattatcac
    27421 ccaatgcaga agatattgta ggaatggcaa aagtttgtga agaagaaggt gctgatggag
    27481 tttcattagt taatactttt aaagcaatgg caattgatat aaaaaataga agaccagtat
    27541 ttgaaaatgt atatgctggg ttatctggac cagctataaa acctatagct ttgagaatgg
    27601 tacatgaagt gtgtaaaaat gtaaatatac ctgtaatggg aatgggcgga ataactaaag
    27661 ccacagatgc tatagagttt ataatggcag gagctacttg tatccaagtt ggaactgcaa
    27721 attttatgaa tcctagaata ggtattgaaa taatagatgg aattaatgaa tttatggata
    27781 gagaaggaat aaaaagttta gacgaaataa gaggaattat ataaggtaga ataaatagta
    27841 ttttaaagat aaaaaatata gattaaataa acaataattt tataacatta acatggagga
    27901 aaaataatat gagtaaagta gatgtagtgg atatattaaa gaaaagtgat gcattgttgg
    27961 agggacattt tttattatct tcaggaaaac acagtaatag atacgtacaa tgtgcaaaag
    28021 tattaagatt tccagaatat gctgctgagg tattaagtac agttgttgaa caaataaaag
    28081 acttagatat agacttagta gtaggaccag ctatgggtgg agtaatagtt tcttatgagt
    28141 taggaagaca attaggaaaa gaagctgtat ttactgagag aaaagacaat acaatggagt
    28201 taagaagagg atttgaagtt aaaaaaggag caaagataat aattgctgaa gacgttgtaa
    28261 ctactggtaa atcaactatg gagacaaaaa gagtattaga agccttaggt ggagaagttg
    28321 taggtgttgc atgtatagca gatagaacta atcatgatat aggtatgcct atatatagtg
    28381 ctataaaact tgatattcaa gtttatgaat ctgatgagtg tcctttatgt aaggaaggaa
    28441 aattaccagt tgttaagcct ggaagtaggg agttcaagga attagggatg taataaaaac
    28501 ttaattattt atagtgttac ttaaaaaatg taaatttttt agagtaaaaa tttgtaatat
    28561 aacatataac ttaaaaataa aaagcagtta gccttataaa tgaaggttag ctgttttttt
    28621 tgttaaaaat atatttacaa aataagagat agtagcagta caatataagt ataaggtaaa
    28681 agtattatat attagggagg ggtaagtatg aaaaatcaat taaatgaaaa tcataaaaag
    28741 gttgtatttt ttggagtagg tgctgtagga gctacttttg cagaacaatt ttttaactct
    28801 aaatatgatt ttaaaattct ttgtgataat gaaagaaaga aaagatattt agaagaagga
    28861 tttataataa atggaaaaag gtatgatttt gattatgtaa ctaaagatga gtataaacaa
    28921 gaggctgatt ttataattat aggtctaaaa tataataatt taaaagaaaa tataaaagaa
    28981 ttagatggat tagttggaaa aaatacagtt ataatgtctc tgctaaatgg agttgatagc
    29041 gaagagataa taggagaaag atttggaatt gaaaaaatgg tatattcata tgttaccaat
    29101 atagatgcaa agaaaatcaa taataatatt atacacacta ctaatgggat aattgtattt
    29161 ggtaacaaag ataatagtga agatagaaaa actaatataa taaccgaagt ctttgatgat
    29221 gtaaatatag aatatacttt atcaaaagat attcaacgag atatgtggtg gaagtacatg
    29281 gttaatattg gtgtaaatca aacttcagct atacttggtg caccttatgg agtttttcag
    29341 agttctgagc atttaagaga attagcaaaa tctgcaatga gggaagttgt tgctatagca
    29401 caagcaaaag acatatctct tacagaagat gatgtggaac attcattaca tagaatacta
    29461 gaacattcaa aagaaggaag aacatcaatg cttcaagatg tggaagctca tagacttaca
    29521 gaagtagata tgttttctaa gaatatctgc aaacttggaa aaaaatataa catacctact
    29581 cctataaatc agactttctt ttatatgata aaagtaattg aaagtagatt ttaaagggtc
    29641 tttgtaagaa atattgataa ttattgattt taaattttat taatgttata tactatttaa
    29701 gttactaaat atttttaact cttcaatgaa aatagttgct gcttttgata aagttatatc
    29761 ttttggaaat atatatgaga aatgtctgtt gtagctgctt ccaagctcta tgacagacag
    29821 ctcattgttt agaacaggta agcttgtcac tagattagag actattgtaa ttcctaaatt
    29881 atttctaaca gattctttga cagcataatt acttccaaaa accatcatac tttttggaat
    29941 aatttcttta acacttaaaa acatatctaa ataatctctg gttccagaac catcctccct
    30001 gactatccat ttttggtttt gaagtttatc aaaactaaaa tcttttaata aatgactctt
    30061 gtatggaagt gctaaaacca tcttatcttc aaaaaaatat tcttgaataa atgaagggga
    30121 agagcaagtg ccttcaataa gaccaatgtc taaaatataa tccttaacat gagaagatac
    30181 gattgatgta ttttttataa aaacttctac atctatgtca ggatactttt tagaaaataa
    30241 tgctagaaaa tttggtaaaa tatactctcc aatagttaaa cttgcaccaa tctttaagtg
    30301 accagttata gcatctgaaa cttggcttac atcatgataa gtaatattta aaagatttaa
    30361 tatttcttta gctcttttgt acaagatttg tccactttct gttataaaaa tggttttttg
    30421 tttaatagac ctgtttataa gcacaacttt aaaataattt tctagatttt ttatgtgttt
    30481 acttacactt ggttgtgata gatttaaata ttccccagct ttagtaaagt ttttgtattc
    30541 aacaacagct acaaatgttt ttaattcttc aaacaatttt ttgtctcctt tacctaatga
    30601 aattttacat tttttatata ctttctacag ataattatac tatatataat aaccattaaa
    30661 aacattaatc attacgatat tatatattta ttttacttat ggttattaca attatatact
    30721 attgctatga agaaaactat attaattata aggtaggtat attatgttaa agaaaaaaag
    30781 cagtttgaca ttaaaaacta taagggaaat attaccagga ctgtttgtat cagtattagt
    30841 aggttatatt agtatattta tttctatttt aattcctaag gtaggggcag catcaatttc
    30901 tatattttta ggtatgtttg taggaaacct atttttaaat caaaaggtat ttcaaaaagg
    30961 ttataaattt tcagagacag atttgttatc atattcaata gtactattag gagcaacact
    31021 cagtgtatct accttaatag acttaaaagt ttcaggaatt ttatttataa tattgcaaat
    31081 gacaataaca ataatagctg cactttatat aggtaaaaaa ctaggatttg aagaaaactt
    31141 tagatttcta atggcaagtg gaaatgcagt atgtggttct tctgcaatag gagcaacagt
    31201 tccagtggtt aatgcgacag ataaagaaaa gggaattgca gtaactattg taaatgttac
    31261 tggtatattt cttatgtttt tacttccaat aatatctcaa tttttatata gccatgaact
    31321 tgtaaaaact tcagcaatga taggtggaac tttgcaatca attggtcagg ttgtagcaag
    31381 tggagcaatt gttggagaaa atgtaaaaga tttagctact atttttaaga tagtaagagt
    31441 aatatttcta gttgttgttg tattagtatt tggacatatg aaaaatcaat ctaataggga
    31501 aattcttgaa gaagaaaagg atgagataaa aaagaaaaaa gtgactatac catggtatgt
    31561 aattggattc tttataactt gtgcactttt ttcaatgaac attataccac aagaagtatc
    31621 tgtgttgtgc aaagaaataa gtaacaaact agaaataata gctttagcag caattggatt
    31681 aaaggttaat gtaaaagatt tagtgaaaca agggaaagaa gtttctttat atggtttatt
    31741 tgtaggtata gtacaagttg tatcagctgt agttttaata aaaatattta tatagcataa
    31801 aaatagtatt tgtaatatta atttgattaa ttaaaataat atgatactaa taatttataa
    31861 tataaataaa aaatgaattt cgataagttt aagtgggtat aaacttatag aaattcattt
    31921 tttatttaag gtaaaaattt aaagtaaaat attattttac ataataataa ccttataaat
    31981 tttgaaatta cttatctttg tgttaaaata agtaaaatgc acatattaga gctaagtttt
    32041 taagtaaata aacaaaatga aaatataaat attaaggaga tttggtatat gaatgtttat
    32101 gaaaaaggta atggagttat actagaagga gtaacagatt ttgacccagt acatatattt
    32161 gagtgtggtc aatgttttag atggcacaaa caagaagatg gctcatacac tggagttgca
    32221 aaggggagaa tattaaatgt caaaaaagag aatgataaaa tatatttaaa taatactaat
    32281 ttaaaggagt ttaacagcat ttggtataat tactttgatt taggaactga ttatacagaa
    32341 ataaagaata agctaaaaaa tatggatgaa tatttaaata aggctactga atttggatgg
    32401 ggaataagaa tattaagaca agatggatgg gaaatgctta tatcatttat aatttcctct
    32461 aataatagga ttcctatgat acaaagagct atagaaaatt tatcaagaaa atttggtaag
    32521 tatataggtg aatatgaagg taatgaatac tatgcattcc caacaccaga agaactaaat
    32581 aaggcctcac aagaggaaat aagagcatgt caaacaggtt ttagagataa gtacataaaa
    32641 agtactacac aggcagttat agagaataat gatgaggtat ctgaatatac aaatttaagt
    32701 actgaggatt gtagaaaaga gcttttaaaa tttaatggag taggaccaaa agtatgtgat
    32761 tgtattgcat tatttggtat gcaaaaatat gattcttttc cagttgatgt ttgggttaag
    32821 agagttatgc aagaatttta tatagatgaa gacatgagtt tacctaaaat gagaacttat
    32881 ggaatcgata aatttaaaga aatgtctgga tttgctcaac agtacttatt ttactatgca
    32941 agagaactcg gtataggaaa ataaattaag ggggagaaat tatgggagta ataggaacaa
    33001 tatttctgtt ctatttaatt atatcatatc tagctggagc cataatttct gtgattatat
    33061 tgcttgaaaa tagagaccct gcaaaaacaa tgtcttggct attgatgttt attatatttc
    33121 caggtgtagg tttaatgata tatgctattt caggtagaaa tattagaaaa agaaaattat
    33181 ttaagactca aaaactagct aataatataa aagaaaagaa actatttgat acattagaaa
    33241 agataacaga aatagttgaa ttagaaaaag aatctataaa acaaaataag cttttaagag
    33301 atgaagagga tggtagttat agaaaaaggg taataaatat gttgttaaag actggtatgt
    33361 ttccctttac taaaaataat aaagtggatg tatttgtaga tggaaatgaa aagtttaaaa
    33421 gacttataga agatataaga gaagccaaag accatattca tttagagtac tttatcatca
    33481 aagatagtga aataggtagg gtgttaaaag aagaacttat aaaaaaggct aaagaaggca
    33541 ttaagataag aattttatat gatgatgtag gatgctggag attttggttt aatagaaaat
    33601 ttttccgaga aatgagggaa gtaggtatag aaattgcagc atttttacct acaaaatttc
    33661 ctataatagg agggaaatta aactatagaa atcacagaaa aattgttgtt attgatggaa
    33721 taattggata tacaggtgga ataaatatag gagatgaata tttaggcaaa aatgataagt
    33781 ttggatattg gagagatact catattagga ttaagggtat ttctgtatac atgcttcaaa
    33841 tgacatttct gattgattgg tattatacga caaaagaagt attagtgact aaaaattatt
    33901 tcccaagtgt agggaatgtt ggtgaaagta tgatacaagt tgttgcgagt ggtccagaca
    33961 gtgactggga agatattcat tatgcttatt tttctgcaat atgtcaggct agaaaaaatg
    34021 tttatataga aactccatat tttattccag atgaaagtct actaaaagct ataaagagtg
    34081 ctgctcttag tggagttgat gtgagaataa tatttcctaa aattgcagac cacaagatag
    34141 taaatatagc atcttattca tattttgaag aaatattaag agcaggtggt aaagtttatt
    34201 tatataataa aggatttata cattctaaag tcgtaataat agatgataaa attgcttctg
    34261 caggtacagc taatatggat ttaagaagtt ttatgcttaa ctttgaggta aatgcattta
    34321 tctatgatga agaagttatt agggtaatga cagatgattt ctttgaggat ttaagtcact
    34381 gtgaagaact taatttggag gtatttaaga atagaaatat catacaaaaa atcaaagaat
    34441 ctgtagcaag attattttca ccaatattat aaaaaattat taaaaaaggt attgaaaatt
    34501 atgtgtgatt ggtatattat aataattgtt agcactctat tagatagagt gataataaat
    34561 aaaaagtata tatataaatc tacatatata agtagtatta atttaaaaat aactaaaaac
    34621 ataggcgaac tagtctatac ttaatatttt aggaggcgtg aataatgaaa ataagaccat
    34681 tagctgacag agtagtaatt aaaaaagtag aagcagaaga aaaaactgca agtggaatag
    34741 ttttaccagg agcagctaaa gagcaacctc aaatagctga agttgtagaa gttggaccag
    34801 gtggaatagt tgaagggaaa gaaataaaaa tggaattgac agtgggagat aaggttatat
    34861 tccaaaaata ttctggaaca gaagttaaga tagaaggaca agaatacaca atactaagac
    34921 agagtgatgt attagctgtt attgaataaa tatagaataa atttattagg aggggtttaa
    34981 aatggctaaa gaaattaaat tttcagagga aacaagaaga gctttagaag ctggtgtaaa
    35041 taaattagca gatacagtaa aagtaacatt aggacctaaa ggaagaaatg ttatattaga
    35101 taaaaaattt ggttctccac ttataactaa tgatggagta actatagcaa aagagataga
    35161 actagaagat agatttgaaa atatgggtgc tcaattagtt aaagaagtag caactaaaac
    35221 taatgatgta gctggagatg gtactacaac tgctacagtt ttagcacaag ctataataag
    35281 agaaggttta aagaatgtaa cagcaggggc taacccaata cttttaagaa aaggaataca
    35341 aaaagcagta acagtagcag tagaagaatt aaaaaatcaa tcaagaatag tagaaacaca
    35401 agaggctata tctcaagttg cttctatatc tgctggtgat gaagaagttg gaaaattaat
    35461 agcagaagct atggaaatag taggtaaaga tggagttata actgttgaag agtctcaaac
    35521 tatgaatact gaattagatg ctgttgaagg tatgcagttt gatagaggat ttgtttctgc
    35581 atatatggtt acagatgtag ataaaatgga agcagtttta aatgacccat atatattaat
    35641 tactgataaa aaaatatcta acatacaaga attattacca gttcttgaac aaatagttca
    35701 acaaggtaaa aagttattga taatagctga agatgtagaa ggtgaagcat tatctacatt
    35761 agtagtaaat aaattaagag gaacatttga tgtagtagca gttaaagctc caggctttgg
    35821 agatagaaga aaagaaatgc ttcaagatat agcaatactt acaggtgctc aagtaatatc
    35881 agaagaatta ggttatgatt taaaagaagc tgatttatct atgctaggta gagcttcatc
    35941 tgttaaggta actaaagaaa gtactacaat agtagatggt tctggagata aaaaagctat
    36001 agaagataga gtaactcaaa ttaaacatca agtagagcaa actacttcag attttgatag
    36061 agagaaatta atggaaagat tagctaaact tgctggagga gtagctgttg taaaagttgg
    36121 agctgctaca gaagtagagt taaaagaaag aaagttaaga atagaagatg ctcttaatgc
    36181 aactagagca gctgtagaag aaggaatagt agctggtgga ggaactgctt ttgttagtgt
    36241 tataccagca ataggaacac taattgagag tttagaagga gaagttaaac taggtgctca
    36301 aatagttaaa aaagcgttag aagagccatt aagacaaata gctataaatg ctggtcttga
    36361 aggtgctgta attgtgcaaa atgttgttaa ttctgaagca gaaactggat ttgacgcatt
    36421 gaatgaaaaa tatgtaaata tgatagaagc tggtatagtt gacccaacta aagttagtag
    36481 aagtgcttta caaaatgctg catcaatagc aagtactttc ttaactactg aagctgctgt
    36541 tgctgacctt cctgaaaaag aagatgtagg aatgccagga atgggtggcg gaatgcctgg
    36601 tatgatgtaa tttaaaaaat ttaaaatata aattttaata taaagaacta tttcgactta
    36661 gattttaaac tctaagttga aatagttctt ttgtatatat ctaatttttg tatatctata
    36721 aattcaatac acatatttag ataatcagta tctagtttgt aataatattt tagagtagta
    36781 ttgttatttt ttaggttatg attttagata tatattaatt aaggtataat tgttgataga
    36841 gaataaatca aatttaaaag gatgcgataa tatggactta agaaaattgc ttgaagaagt
    36901 taaaaatgaa aatattgata tagattttgc gttagaaaag ttaaaagatt taccttatga
    36961 agaccttgga tatgcaaata tagaccatca tagagaatta agaaatgggt atccggaagt
    37021 tatttattgt gaaggtaaga gtgatgagca tattttaggg attattgata aaatgaatca
    37081 aaaaagttct aacatattgg ggacaaggtg cagaaaagaa acttttctga aaattaaaga
    37141 aatctataat catgctgagt acgaagaggc gtctaaaatt cttaagattc aaaatcatga
    37201 tatagagaat ataggaaaag gtaaaattgt gattgctaca gggggaactt ctgatatacc
    37261 agtagcagac gaagcttatc atacagctaa gtttttaggt aatgatgttg atagaatata
    37321 tgatgttgga gtggcaggta tacataggct tttaaataaa agacataaaa tagatagtgc
    37381 tagagtaata gtagccgttg caggtatgga aggtgctctt gcaagtgtaa ttggtggact
    37441 ggttgatgtt ccagtaatag ctgttccaac ttctgttgga tatggagcaa attttggagg
    37501 attagcagca ttactagcta tgttaaatag ctgtgcatct ggaatatctg ttgtaaatat
    37561 tgataatgga tttggagcag gatatttagc atcaatgata aataaactgt aaatacattg
    37621 atataaatta aacaaaacta tataataaaa tttaatttct attatatttt tactattaaa
    37681 attgaggaga catatatgag tgaaaaaata ctttattttg atataataag cggtatatca
    37741 ggggatatga cccttgcaag tttattaaat ttaggtgtgc ctaaggaaat ttttttggag
    37801 gaaattcata aactaagaat gagcgatgaa tttaatataa acatcagctc gaaaactgaa
    37861 aatggaatag taggtacaaa agtagaagta attacaaaag aaaaacatac acatagaaat
    37921 ttagtggata tatttgaggt tatagataaa agcactctaa acgaaaatgt aaaatctaaa
    37981 gcaaaaaaaa tatttatggt aattggagaa gcagaagcaa aagttcatgg aactacaata
    38041 gacaagatac attttcatga ggtaggggct atagattcaa ttgtagatat agtaggagct
    38101 tctatattag ttgacttact ttgtgtagat aaagtttgtg ctagtacagt gtctgttggt
    38161 tcaggatttg taaaatgtga acatggaatt atacctatac ctgcacctgc aactatagaa
    38221 attttaaaag gtgtgccaat taaattaaat aatgtaaatg gagaatgtac aactccaaca
    38281 ggagctgcaa tcataaaagt cttgtgtgat gagtttgtag atgagcttga attttctcca
    38341 aagcaaattg gatatggtat aggtcataag aagtttgaag taccaaatat actcagaact
    38401 gtattaggtg aaaaaaaaaa gaagaattaa tttatgaaat tagtgcaaac attgatgata
    38461 tgtcttcaga aatctattct tatttgtatg aaaaaatatt agatgagaaa gcattagata
    38521 tttatacaga gagcatttat atgaagaaga atagaccagc aacaaaagta tcaattttat
    38581 gtaatgaatc agatttagaa aaatttatta aaatattact tttagaaact agtacttttg
    38641 gagtgagata taatgcatat aatagagaaa ttctagagag aaaatttgta aaattagata
    38701 caaaatatgg agtggttaca gtaaaactag ggtattataa aggaaaacta gtcaaggcta
    38761 ctccagaata taatgaatgt aaaactatag caaaaaatga agatattcct ataaaaaaag
    38821 tgtttgctga tataaatttt agaataaaag aagaatttag tgaaaactta ttgacataag
    38881 taataaattt tgataaatta atattcagcc ttattaaata aaagtaaaca gaaaaaaata
    38941 tataaaatat aaactcactc gtatatgctc agaaatatgg tctgagagtc tctaccaaga
    39001 taccgtaaat atcttgacta tgagtgaaat tattatacca aaagacctag ttaagtaaat
    39061 gtgtaggctg agttaaactc cctatgtgtt ggctataaga taaaggtatt ttggaagcct
    39121 gtatagtaat ttcacttttg ctagaaatta tggaagttac tatacaggtt ttttgtgtaa
    39181 aaagatttaa tataatttag actgtattgt gttaaaataa atatataaaa actatattat
    39241 acaaggagag atgtaaattt atgaaacatg aattagtact tgtaatagat tttggaggac
    39301 aatataatca gttgatagct agaagagtta gagaaaacaa cgtatattgt gaaatacttc
    39361 catgtactgc aagcatagaa agaataaaag aaaaaaatcc taaagggata atatttacag
    39421 gcggtccaaa tagtgcgtat ttagaagatt ctccaacaat atcaaaagaa atatttgaac
    39481 taggagtacc aatacttgga atatgctatg gtattcaaat aatgtctcat gtattgggtg
    39541 gagttgtaag aaaaggtaat aaacaagaaa aagaatatgg aaaaacagct ataacttatg
    39601 gaaaatcatc attatttgaa ggaataacta caaatagtgt atggatgagt catacagatt
    39661 taatagaaaa agttccagaa ggatttacta tagttgcaaa tactaatgat tgtccagtag
    39721 cagctatgga aaatgtagaa cgaaacctat atggagttca attccatcca gaggtagaac
    39781 actgtttgga aggggacaag atacttacaa acttcttata taatatttgt aaagtaaaag
    39841 gtgactggac tacagattca ttcatagaag ataaaataaa agaattaaaa gaaaaaatag
    39901 gcgataaaaa agctttatgt gctttaagtg gaggagttga ttcttctgtt gctgcagttc
    39961 ttattcataa agcaattggg gacaacttaa cttgtatatt tgttgaccat ggtttactta
    40021 gaaaaaatga aggaaatgat gtagaaagaa tatttagaga gaaatttgat ataaatttaa
    40081 taagagttaa tgcagaagat agattcttat ctaaattaaa aggagtttct gaaccagaag
    40141 ctaagagaaa aataataggt gaagaattta taagagtatt tgaagaagaa tcaaataaat
    40201 taggaaaaat ggatttctta gtacaaggaa caatctatcc agatgttata gaaagtggac
    40261 atggaaatgc tgctactata aagtctcatc ataatgttgg tggaatccca gaagatgtag
    40321 acttccaaga aattgtagaa ccattaagag agttatttaa ggatgaagtt agaaaaatag
    40381 gattagagct tggtatagaa gaaggattaa tatttagaca tcctttccca ggaccaggtc
    40441 taggaataag agttataggt gatgtaacta aagaaaaatg tgatatatta agagaagctg
    40501 atgctgttta tatggacgag cttagaaaag caggtttata cagagaaata tggcaagctt
    40561 ttgcaacatt accagatgtt aagactgttg gagttatggg tgatgaaaga acatatgctt
    40621 atttagttgg tttaagagca gttacttctt ctgatggaat gacttctgac tggtataaga
    40681 tgccttatga tgtattagaa agaatttcta atagaataat aaatgaagtt gatggagtaa
    40741 atagagtagt ttatgatata acttctaaac ctcctggaac tattgaatgg gaatagtaaa
    40801 taaaaaagtc gtaagccttg tattttcaag gtttgcgact tttaaaaaaa tctttgtgat
    40861 actaaaatga tactatatat ttaaattatc tagtttttgg gcaacttcaa gttgcttatt
    40921 tgggtataaa tgtgaatatg tattccaagt tgtttctact ttttcatgcc ccaatctttc
    40981 tgctatagtt aatatattta catccatatt taccaataat gacgcatgag aatgtctcaa
    41041 atcatgcact ctaattcttt ttacattaga taacttacag catctatcta gctctttaga
    41101 aagataactt ttagcaaatt taaatattct ttcattatct tttaagtcat atagcttaga
    41161 tagataatct tttatattat tatataagaa atgtgggata gggacaacac gtttactttt
    41221 aggagtttta ggagaagaaa caatatcctc tccatttagt cttatgtagc ttttttcgac
    41281 gcttatttta ttttcgaaaa tatcttttgg agtaagagct aacaactccc ctagtctaag
    41341 acctgtccaa aataaaattt caaatgctag tctaggttct gatttctttt caaattcaat
    41401 aaatttttta aactcttcta atgtccaaaa gttcatttca tctgcgtttt ttttaccgat
    41461 agaacctgcc aaatgagcag gattagaagg caagttataa tatttaactg cataatttaa
    41521 tacagcaaca agctggttgt ttatcgtttt aatatatgtt tggctataat cactttttaa
    41581 taattcgttt tgccatcttc ttatatgtgt tgcagtaatt tcatttattt ttagactttt
    41641 aaaaaaaggt aaaattctaa agtttattaa atacttttta gtttctaatg tagataattt
    41701 taatctagaa gacatatcgt gcatgtattc ttctatgaga ctttcaaaac tcatatcagt
    41761 actcatttta gatttattca aaaattctct ttcaaactct agagcttctt ttttagtttt
    41821 aaaacctctc ttaattttct ttttcctatc cccatcgaag tctgtaaaat aaaagctagc
    41881 ataccaactt tttctttgtt cgtctttgta agcaggcatt ttagacaccc ccaattatgt
    41941 cattttaata ttacaataat tataacatgt tagatatgct atctaaataa ttgttttatt
    42001 ttcctcttct ttttatttct tgaagcttgt aatatttaca cagttctaag aagtcttctt
    42061 ctagtgctat agataagtct aatatcgtag atatacctat atctttgtac tcttgatttt
    42121 ctagttttga tatataactt ctgttacgtc tcattttttc tgctaattct agttgtgtca
    42181 ttcttttctt ttttcttaat tttttcaaca tttattaaaa tccacctttt aattattttt
    42241 atgacatttt tataattaat agtttgtgta atttcttgta aaaaatgttc ctcattagaa
    42301 cattttttgg tggaaaaatg tgttaaaatg taagtaagaa aagcactttc aaagcactat
    42361 aaaaatagtg ctgaaataga taaacaaata aaagtaataa ggaggaaaga tagattctag
    42421 agggaaatca ttatcatttt taagaacgta tgttcagttg gtgggataga aaaatacttt
    42481 acaggggatg gtttatttga atgagaatga aaatatgagt ttatatgtca gtaagttaaa
    42541 agaagtattg aaaaataatt cagaagagta taaaaaaata agagctaaaa taaatgaaat
    42601 ttataatcta aatgaaaaga aagaataaaa gggctttagc tcttttattc tttcttagta
    42661 ttaactgctt tataataagc attatctatc atttttttta ttgcattttt atcatctgaa
    42721 tctaaagatg ctaatctatt tattagttcg ttaacttcat tatcttcttt tgagttaact
    42781 ttttttaata attcatcatc ttgttgagtg ttaggttgta ttttacctaa tttagttaat
    42841 agctccttta aagttaaatt taatccaaaa ctcatttttt caagcatatc taaagtaggt
    42901 tcaacagctt taccattcct tgggtctctg tttttttcta tcttatctat atatgtatgg
    42961 ctcacaccac aaagattaga aaattctcta agagataagt tatgttcact tctatattct
    43021 ttaataattt ctgctaatgt ttgcataatt tcacacctca cctaaataat agttgactta
    43081 attatatcag aaaaatgtaa actattgtta acaaaaataa aaatttagta aaaaataatt
    43141 gacaagagta aaaaaaagac gtatactatg attaacaaaa aggttataaa taaagttagg
    43201 agggattaat ataaaaaata atttacaata tattagaaaa gaatctagaa tatcacaaaa
    43261 aaaattcgct gataagatag gtatatcaag accgtattta tctagaatag aaaacggtaa
    43321 ggttaatccg agtttagaaa tagcgcataa aatctcgctc gaaacaggga aaacaataaa
    43381 tgaaattttt tttgatttta ctgtaaatca tagtaaacta taaggtttta attgtacctt
    43441 gaaaactaaa tataaaatat tttaaaggag caagtatatg aaaaataaag aaaaagaaat
    43501 aatcattaga ggaattttaa taggaatttt ttatttttta ggaattgtat ttagtaatac
    43561 tttttttaaa taataaatag aaggggtagg tgaaatacca actccccaaa tcaaaataac
    43621 actttggaag ttaaata
//
LOCUS       DAMJWU010000024        43166 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMJWU010000024 DAMJWU010000000
VERSION     DAMJWU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43166
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.024"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(<1..1876)
                     /locus_tag="PC460_001437"
     CDS             complement(<1..1876)
                     /locus_tag="PC460_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600329.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     T"
     gene            complement(2059..3534)
                     /gene="spoIVA"
                     /locus_tag="PC460_001438"
     CDS             complement(2059..3534)
                     /gene="spoIVA"
                     /locus_tag="PC460_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF2600330.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3706..5001)
                     /locus_tag="PC460_001439"
     CDS             complement(3706..5001)
                     /locus_tag="PC460_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2600331.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(5185..6594)
                     /locus_tag="PC460_001440"
     CDS             complement(5185..6594)
                     /locus_tag="PC460_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2600332.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(6646..7785)
                     /locus_tag="PC460_001441"
     CDS             complement(6646..7785)
                     /locus_tag="PC460_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2600333.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVEGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            8104..8754
                     /locus_tag="PC460_001442"
     CDS             8104..8754
                     /locus_tag="PC460_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2600334.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(8911..9930)
                     /locus_tag="PC460_001443"
     CDS             complement(8911..9930)
                     /locus_tag="PC460_001443"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2600335.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(9961..10575)
                     /gene="plsY"
                     /locus_tag="PC460_001444"
     CDS             complement(9961..10575)
                     /gene="plsY"
                     /locus_tag="PC460_001444"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF2600336.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(10610..11929)
                     /gene="der"
                     /locus_tag="PC460_001445"
     CDS             complement(10610..11929)
                     /gene="der"
                     /locus_tag="PC460_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF2600337.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(11963..13291)
                     /locus_tag="PC460_001446"
     CDS             complement(11963..13291)
                     /locus_tag="PC460_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF2600338.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADTHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(13529..13729)
                     /locus_tag="PC460_001447"
     CDS             complement(13529..13729)
                     /locus_tag="PC460_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600339.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(13731..14663)
                     /locus_tag="PC460_001448"
     CDS             complement(13731..14663)
                     /locus_tag="PC460_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2600340.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(14708..15640)
                     /locus_tag="PC460_001449"
     CDS             complement(14708..15640)
                     /locus_tag="PC460_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2600341.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(15695..16984)
                     /locus_tag="PC460_001450"
     CDS             complement(15695..16984)
                     /locus_tag="PC460_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2600342.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(17013..17705)
                     /locus_tag="PC460_001451"
     CDS             complement(17013..17705)
                     /locus_tag="PC460_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600343.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(17720..18481)
                     /gene="pgeF"
                     /locus_tag="PC460_001452"
     CDS             complement(17720..18481)
                     /gene="pgeF"
                     /locus_tag="PC460_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF2600344.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(18478..18939)
                     /gene="nrdR"
                     /locus_tag="PC460_001453"
     CDS             complement(18478..18939)
                     /gene="nrdR"
                     /locus_tag="PC460_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF2600345.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(18961..19224)
                     /locus_tag="PC460_001454"
     CDS             complement(18961..19224)
                     /locus_tag="PC460_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2600346.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(19328..20101)
                     /gene="sigG"
                     /locus_tag="PC460_001455"
     CDS             complement(19328..20101)
                     /gene="sigG"
                     /locus_tag="PC460_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF2600347.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(20193..20915)
                     /gene="sigE"
                     /locus_tag="PC460_001456"
     CDS             complement(20193..20915)
                     /gene="sigE"
                     /locus_tag="PC460_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF2600348.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(20940..21812)
                     /locus_tag="PC460_001457"
     CDS             complement(20940..21812)
                     /locus_tag="PC460_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF2600349.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(22102..23376)
                     /locus_tag="PC460_001458"
     CDS             complement(22102..23376)
                     /locus_tag="PC460_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2600350.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(23660..24817)
                     /gene="ftsZ"
                     /locus_tag="PC460_001459"
     CDS             complement(23660..24817)
                     /gene="ftsZ"
                     /locus_tag="PC460_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF2600351.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(25007..25360)
                     /locus_tag="PC460_001460"
     CDS             complement(25007..25360)
                     /locus_tag="PC460_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF2600352.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(25459..26133)
                     /locus_tag="PC460_001461"
     CDS             complement(25459..26133)
                     /locus_tag="PC460_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2600353.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(26138..26836)
                     /locus_tag="PC460_001462"
     CDS             complement(26138..26836)
                     /locus_tag="PC460_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2600354.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIEIYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(26859..27599)
                     /locus_tag="PC460_001463"
     CDS             complement(26859..27599)
                     /locus_tag="PC460_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF2600355.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(27869..29095)
                     /gene="murG"
                     /locus_tag="PC460_001464"
     CDS             complement(27869..29095)
                     /gene="murG"
                     /locus_tag="PC460_001464"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2600356.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(29113..30243)
                     /gene="spoVE"
                     /locus_tag="PC460_001465"
     CDS             complement(29113..30243)
                     /gene="spoVE"
                     /locus_tag="PC460_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF2600357.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(30295..31650)
                     /locus_tag="PC460_001466"
     CDS             complement(30295..31650)
                     /locus_tag="PC460_001466"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF2600358.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASVIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(31669..32637)
                     /locus_tag="PC460_001467"
     CDS             complement(31669..32637)
                     /locus_tag="PC460_001467"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF2600359.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(32678..34051)
                     /locus_tag="PC460_001468"
     CDS             complement(32678..34051)
                     /locus_tag="PC460_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF2600360.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(34218..36197)
                     /locus_tag="PC460_001469"
     CDS             complement(34218..36197)
                     /locus_tag="PC460_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF2600361.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(36209..36556)
                     /locus_tag="PC460_001470"
     CDS             complement(36209..36556)
                     /locus_tag="PC460_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600362.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(37042..37977)
                     /gene="rsmH"
                     /locus_tag="PC460_001471"
     CDS             complement(37042..37977)
                     /gene="rsmH"
                     /locus_tag="PC460_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF2600363.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(38007..38753)
                     /locus_tag="PC460_001472"
     CDS             complement(38007..38753)
                     /locus_tag="PC460_001472"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF2600364.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(39147..40433)
                     /locus_tag="PC460_001473"
     CDS             complement(39147..40433)
                     /locus_tag="PC460_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2600365.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LEYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(40448..41713)
                     /locus_tag="PC460_001474"
     CDS             complement(40448..41713)
                     /locus_tag="PC460_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2600366.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSSKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            42029..43126
                     /gene="ychF"
                     /locus_tag="PC460_001475"
     CDS             42029..43126
                     /gene="ychF"
                     /locus_tag="PC460_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF2600367.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        13440 a         7950 c         4530 g        17246 t
ORIGIN      
        1 gtgttagtat acaaaataaa aaaataatat ctttttttag attatattct ttagattcta
       61 atttataatt attatttatt attccatcat aatcacttat atttgactta tttttatttg
      121 tatcttgatt tttgttagaa atactttccc tataaggaaa agaatgttca ttggtaccaa
      181 atactgattt tgagcttaaa tcataattat catcattttc tgtaattgaa tttttattgt
      241 aaaatccttt ttctttatca gaattatttt ctttattacc gtctttaggt atatcatgag
      301 gatttttatt ttcattgcta tcagtacttg attctacatc atctttagga gtatttggtt
      361 gtttctcatc agaattatca ttttcattat tattattttc attattattg ttttgattgt
      421 tattactatc attattttca tttccattat tcccatcttg attgttatca tcctcatctc
      481 tatccgggtc gtctctacct cctcctccac ggtcaccatc aatatcatca ttttcccgtt
      541 catcatctgg cgtctcttga ttgtctccgc ctcccattat aaatcctggt ttaaaaacta
      601 tatttgaata cccttctttt aatcctccta ccacttttac tctaaaacaa cgcaacttaa
      661 aatcttcaaa aaaaagtata gcttgatctc taatatcctc ctgttcttca gatatccaat
      721 tgattccatc gcttgaatat tcaacaaaaa ccttacttgc actatacggc atatcataaa
      781 ataaaacagc tacatatcca ttttttgtat tttgaaattc cagttccaaa ttgtctattg
      841 gttttttctc tataacagat acattcaaaa taggaactac atcatcattt attataattt
      901 tttctttatt taacatttct gttttaaact ttcctgttat attaaaattt tctccctttt
      961 ccaacttttc ataataatca gtaaaatccc attctacatc tatatcaagt cgattaccac
     1021 tagatgtttc taataccata tatttgggaa attttatatt ttctatttta tcatctttac
     1081 ttacttttat ttcatcacta aatacaccaa ttcccctatg acaacatttc acaatatcat
     1141 agccagaatc ttcagctata gaaggtataa gctcattata atcatcaatt atataaactt
     1201 tcttactttc tgatataatt gattgtgcca acttattatt atctcttagt aacccacttt
     1261 cattgttgct actatatgct tttattgaag tttcttctat ctcaacatca cctttagaat
     1321 aaacacctat agcatgttct ccattcactt caatatcctt atttagtatt ctaatatcat
     1381 tttcagtata aattcctatt gcatcttttc catttgcctt tattttttca acagattcgt
     1441 aggatgattt aatatgtaag tctcctcctt cttcaacgta taaggctgtc ccatcagtag
     1501 caattatgtt gtttatacca atacttatac ttccaccatt ttctatatgt attactcctt
     1561 cttttcctcc ttcacctatt atattcaatt cattatcatc aataataaat ctcccatgat
     1621 tttttatcaa tattttatat ttgttggtat ctattgtaaa attactgtca taaggaatat
     1681 aaaatctaaa ctcttcatct attaccatat cacaagaaag agtataaact atttcttctt
     1741 tattattttt attttcttga acccaccttt caaaatcttc aagacaattt tcctcttgtc
     1801 caaaaacttt atgtggaaaa cttataaaac acaaaactat tatactcata aaaataaata
     1861 tctcatactt tttcatctta cactcctaga aatttttagc tttgaaacaa tcctgtcgaa
     1921 acatatactt cacccactat aatttttcca caattatcaa ttattgtcaa tttatataag
     1981 tatctttatt aattgttata aataaaaagc tacttactaa agcaaacaga cgtttcactt
     2041 taataagtag ctatattttt ataacaaaat agttataata ttagaacttc cttcatttac
     2101 aattttttgt aaagtctttt gcattttaac tcttatctct tctggcatag tatataattt
     2161 actttgtaat tgctctttta ctaagtcatg tagtgattta ccaaacatat ttgattccca
     2221 taaatctgct ggttgctctt caaatacttc cataagatat tttatcatct cttcaccttg
     2281 attttgattt ccaactattg gagaaacctc tgtagatata tctgctttta taatatgaag
     2341 tgatggtgca ttggctttta acttgatacc atattgtttt ccttgtttca ttatttctgg
     2401 ttcttccaga ctaagttctt ctaaggaagg agctactact ccatatcctt ttatcttggc
     2461 atctattaaa gcactttcta ttttatcata ctcattttta actttagata gtctagttat
     2521 cagactaagc agttgataat ctccttcaat tttaaatcca cttttttctt caaggacatt
     2581 atagaataaa tcttgctttg tctgtaaatc aatatttata acaccttctc ctagctctac
     2641 attatcaaca cctgtatctt ccaaaaactc taactcagaa aatccttgta taataccttc
     2701 tatatccctt attttaccta tatctataat actttgtttt aatgttgtta ttatgctact
     2761 ttttatccaa tgatttcttt caagaccttc aacccatttt ggtaagttga ttcttatttc
     2821 tgtaagtgga aaatcatata atacagcttc cataacctcc tctatatctt cttcttccat
     2881 ttcaaccaca ttcattggaa gaactggaac ttcatacttt tcttccaatt cacttctaag
     2941 catactcgtt tcctcacttt ttggacttaa cgtatttaaa accactgcaa atggtttttt
     3001 aagatttttc aattcttgta taactctctc ttctggttct acatagcttt ttctgtcaat
     3061 tccagtaact gaaccatctg tcaagacaac tatccctatt gtagaatggt cttttataac
     3121 ttttttagtt cctatctctg ctgccttttc aaatgtcata gcttcttgtg accaaggagt
     3181 ggatactaat ctttgttttc caccttcctc atgccctaaa gctccttcaa ctatgtatcc
     3241 aacacaatct accattctta cttttaaaga tactgtgtct tttattttta tttctacacc
     3301 atctgctggt acaaattttg gttccactgt cattatcgtt ttacctgaac cactttgggg
     3361 tatctcatct cttgttctat cctttttaaa ttcattgtct atattaggta taaccaactt
     3421 ttccataaat tttcttataa aagttgattt tcctgttctt acaggtccaa ctactccaat
     3481 atatatatcc ccctgagtcc ttttggatat atcttcgtat atgttattat tcatattatt
     3541 cctcctatac atctaatctt attaaattat atttagagtt aagggttaat attcttaaaa
     3601 tagaaaaata aagaccagca atattaattt aatttacata tagaaatttg taaatttcaa
     3661 tgtaaattaa attaatattg ctggtccaaa tagtagtgta ttatattaac tgactttagt
     3721 agtattttca tttgaattat tattcatctc aataagttct ttacgtcttt cttttgtcat
     3781 gttcatccat ataaatgtaa acaccaatcc aattatggct actatagtaa gaactatgaa
     3841 gtagtaccta tatccagtta cacccggaaa tgcatccaat atcttccctg ctgctaaagg
     3901 acaaagtact tctggaagat atcctaatgt agcaactatt cctatagcag ttcctgatat
     3961 tgataatgaa taattacctt cttcaagtaa cgaatagtgt aaaccttgaa ttgcatacat
     4021 agatacatat attgcaacac atccaatcaa tagtatataa atcatagatg attttgttgg
     4081 tgtaaatatt acacctaaaa gacctgcaat cataagtata aatccataag atataatctt
     4141 tgatgaacca actttatctc caagtaatcc agaagcagct gatgctactg gtctacaata
     4201 ctgagctaat attgagattg cagatgcaaa caccacagta gctccaaatg cttcagttga
     4261 ataaggcgta aaataataaa atgacatatt agctgaatat gaacaaaaca ttattattgt
     4321 tattatccat gtatgaggca tttttattac tgcaaaaaag gcttctttat caaattttgc
     4381 tgatacctct ttttcattat cctttaattt gaaaaatact aatactccaa ctattacatt
     4441 tattgccgag tataatacta taatagctgt aagaccagat ttatcactta cttttgatga
     4501 aatataccca aatagtatca atactagaga tagatgaatt gcattggtaa ttccacgacc
     4561 actttccata aatccaaaag cttttccttg ttcattttca gatgctaaca ttctaacagc
     4621 ttttacaaga gctggccaaa aagtaagtat tgttgttatt ccccataagg catggattaa
     4681 aaacacaaca atatatggag gataaagtgt taatacaaga ccagatattc ctgtaataat
     4741 taaagaacct gaaagcaatt ttctggctga aatacggtct gctaaaaaac caccaaaaag
     4801 gtatgatacc attgcaagta gaccataaat acttcctaag cttcccattt gtgtatttgt
     4861 aagattaaat gcttgtgcaa aagtatcata ataataattc tttaaatatg gtaaagcata
     4921 tataaatgct cctgctacaa agagtattat caagactttg aaatttttcc aaatgcttgg
     4981 tttatttact tcaactgaca tacttcctcc ttatgtaaat acctgtaaat tttcatgata
     5041 attaattatt gcttattgta ttgtttattc aatatttcgg aatatttatt aatatattag
     5101 gttaatctat caataattta tggtgagtta tgagctaaaa gtaaactctt ttagctcacc
     5161 ataataatta tatttttatt tatactaacg tccagagtta ttagatatct ttttttcata
     5221 gttcttccat agcattaaac ctgttattat aacaaaaaga cctcctccta tcatttcaat
     5281 ctcatagatt atattatctt ttaaagtctc cataactggt gccgttgaga aaagtatgcc
     5341 aactacagtt actacaagta ccattctggc aatattgata caaactgaat cactttttgc
     5401 aattgaatat ggtctttctt catttggtct tgtctttcta agtctaatat aagatgtaaa
     5461 aagcaagaca tatggaaata atgctgttaa tgctgtcata gtaacaagaa cattgtatat
     5521 attatctact gatggtaata agtttgttcc tacaagaagt agactcacta ttatagcctg
     5581 aactattact gcatttgatg gtatattgta tttatttgta acagtcaatt gcttaggaaa
     5641 tacaccttct cttacatttc caaaaagcat ctttactgga cctgctatat aaagtattaa
     5701 tgctcctagt actgataaag ttattccaaa tgctactatt cttataaacc aacttcctat
     5761 acctaaatct tgtgcaactt tagctaatgc atctagtatc cctgtagatg ctgcaatttt
     5821 atctggagga agtatcattg ttattgctac tgaacctaag atatagattc ctcctattaa
     5881 aacagccaca gttaatatag ctttaggaaa gtttttctta gcattatcca tttcggttat
     5941 gaaattagca gtcgtttcag ctcctgataa tgcaaatatt atagcagata tagaaactaa
     6001 tgaattggca tctatttttg gtattatatt ttggattgta tatatcgatg ctgatggagc
     6061 ttttttgaga ataactacag acataaatgc catcactata agtaatatag caggaattgt
     6121 agaacctaat gctcctgtat tcgtaaaaaa ttttccaaat gccatcccac gagtacttac
     6181 taaggacaac agccaaaata ttatgagtga aagtgctaaa ataaacatct tattgtttga
     6241 taaatctgga tttccaagca tatatgcaat atttatagct aaaaatgtca agaaagaaga
     6301 ataccaaaat atctttgatg tccaatttag ccatgacacc ataaatcccc acttctctcc
     6361 ataagcttgt ttaacccatt ctccaagtcc accatctttt ggatatgttg cagccaactc
     6421 tgcacatata agtgatgcag gaacaaagaa tataaaagca aacaataacc aaactggtat
     6481 agaaccaaga cccaatccaa aactagaggc agtagattta gctatccatc taatcccaaa
     6541 tagtgctgat atattcataa atactaaatc ccaaaatcca attttctttt tcactgtaat
     6601 tcctccaatt tcaaatttat tcaaactcat acactatgta ttgatttaaa tatcaagtat
     6661 aagttcttca acacttcttg gataattagt tataattgta gcaccattct tacctactat
     6721 tgctgtatct gagtgtctaa atcctccaac tccaggtata tatatccctg gttccataga
     6781 aaataccatt ccttcttcta atattaattc attgtcaaat cttaaatatg gctcttcatg
     6841 ttctgacaaa cctaaaccat gaccaattct atgatttgaa tataactcta gaccatactt
     6901 tgcaacaaca gctcttgctg cttcatccac cattcttgct ggtataccag cttttattgt
     6961 atctaatcca gcttgttgag cctctactgc aatcttaaac acttcttttt gtctttctgt
     7021 tggtttgcct ataataaaag ttctttcatt ttctgctcta tagttttcat accaaacctg
     7081 tctactatgt attactatat ctcctctttg aagtattcta gtattagaat ctaaatgagg
     7141 ttgtgctgtt ctatcaatcc ctgaacaagt ccagtttgca aatcctatat atgtatcagg
     7201 ataattttca gaaacatatt ttagtaaagc attatcccct tcaacgtcaa actccaattc
     7261 actcatccca actctaacat tttctaaaga cccttttatt cctatatcag ataggtaacc
     7321 tgctatcttc aaaaattcta tttcttttgc atcttttaca tatctcattt caaatatttt
     7381 acttccaaca tctttgatag caaatgactt atcagttata tactctgaaa agcttgcaga
     7441 tactatatct ttttctattc caacagttgt tcctttaggg tatttattta aaattatatc
     7501 taagtaatgt ttatgtgata ttccatgttc tttcatttct ggtatttcat aatacacata
     7561 tacattatca acttttgcaa cttctttagc atgtaattct tctaatccag gtactattaa
     7621 ttctatttta tcttgagtta ctactgttac aattggtcta gaataagtta ttgctttaaa
     7681 tccactaata taaaattgat tgtctggttc aaatattaaa gcaatttcaa tgcttttttc
     7741 attcatgtac tttctcaatc tttctagtct catattattt gacataatgc acctctttct
     7801 taatattcag ataatttatt caatacaaca aggatgaata tattatttta aaattcttaa
     7861 tatttttagt acttgtcgta tttgtatgta aattaatttt atttgtgatt attttatcat
     7921 aatcatgatt attttgtcaa tcatgatttt attttaaaaa attttttata ttctagtgat
     7981 tttaaattat ctaaaatata acgagtatac attgtatttt gtaatattat tcttgattta
     8041 tggtaagttt aatgataaaa tcacgattat ataaactata ttgattattt gtaggtgaat
     8101 aaaatgacaa ataaagaatt acaaaagaaa aggatattga tgtatttcat agaagctgct
     8161 caaaatatta tggaaaacga aggtatagaa aatattacac ttcgtaaggt tgcagatatg
     8221 gctggctata atagttcaac tctatataaa tactttaaaa atttagacca tttaatatca
     8281 tttgcatcta taaaatattt taaagattat aacttgaata tttcaagatg tatagaaaat
     8341 gtcaatgatg aatataagaa atatataatt atgtggaaat tattttgtaa gcattctttt
     8401 gataatgctc aagcttttta ccaggtattt tttaacttgt ctagtgatga acttagctat
     8461 ataacaaaac aatattatga tatgtttcca gaagatttag gtattcatga tagtgacata
     8521 tctttaatga ttacaggaga aagtataaag gaaagaaata agatactatt aaacaatctt
     8581 gtagatactg gatatatacc agctaaagat ttagacataa tcaatgatat aattatttct
     8641 ttttatcaaa atctattatt cttaaaaaag aacacaggaa aaaattctga tgatgatgaa
     8701 ttaactatga aaatgatttc ttctattgaa tacataataa aacatgctaa ataaaattaa
     8761 aatatggctg tctcaaaata gagataagcc ttttttattg ttgaaataat aaaaatatat
     8821 ttaatttcat ataaaaatga gtgcctcgta aactaaaaag ttcattttga gacaccccta
     8881 aaataaattt attatttatt atacaatagt ttatatatca tcacctaaaa ctacttcttc
     8941 catttcatgc tttttacttc tcatcattag ttctattcca acttctttag gatttgaacc
     9001 tttctttatt acagaatata tagcattagt tataggcata tctatatcta atttttcagc
     9061 aacatcatgt gctacttcag tagcagttat gccttctaca accatcttta cttcttttaa
     9121 agtatcttct aaactcatcc ctttacctat gagtatacca gctcttctat ttctactatg
     9181 catacttgta caagtaacta ttaaatctcc aatacctgaa agacctgcaa aggtagacat
     9241 attagctccc atagcaattc caagtctgct catttcacta atacctcttg tcataagggc
     9301 agcttttgca ttatcaccat accctaatcc gtcacatatc ccagctccaa atgctataat
     9361 attttttaat gctcctccaa gttctactcc aactatgtct ggattcgtat aaactctaag
     9421 tttagggctc ataaacaaat cttgtatcat ctgtgctatt tttaaatctt tagaagcaac
     9481 tactacggta gtaggaatat cccttgctac ttcttcagca tgagatggcc cagatagtat
     9541 tacatatgga ttttgtggta attcttcttc acagacttgt gataatctaa gcccagtccc
     9601 tttttcaaga cctttagcta cattaacaat aacttgtcct tcttttataa atggttttat
     9661 tttttggcaa gtacttctta cagcctgtga tggaactgct aaaactatta ccttacaatc
     9721 ttttatagtt ttttctatat cagtacttat agttatatta tttggaaata atactcctgg
     9781 taaatagtct gtattttctt tagttctgtt tattttttta gcttgttcct cttttcgagt
     9841 ccacatgctc acatcattac ctttttttgc aagccctaaa gctaatgcac ttccccagct
     9901 tccagtacct aatacacata ctttctccat gtttaacacc acctgagtat aatatattta
     9961 ctattctttc ttttgaccaa tttttctctc tgtcccattt aataatcgct ttatatttgc
    10021 tctgtgatta tatattactg atgcagttaa gaacagagct actattaaac cagctttatt
    10081 ttttaccata atcataaata ttggtgaaca tcctattcct acaacagaac ccaatgaaac
    10141 atactttgtt atagctacca ccaatataaa tactgctaga cacattagtg ttattactgg
    10201 atttacagct agcattgccc ctagtgaagt tgcaacacct ttgcctcctc taaatcctaa
    10261 aaccgctggc caattatgac ctgctacaac acatataact gccagatatc cagctaaaag
    10321 agtatctatt ccaaccaatc ttgctgcaaa ttctgatatt aaaacagcca ttacaccttt
    10381 aagaacatca cctaaaaatg tcattgctcc tgctcttttt ccaagtgtcc taagtacatt
    10441 tgtcgaacct gcattaccag aaccttgagt ccttatatca accccagcaa ttcgctttgc
    10501 aactatgtat gaggtagata tatttcctaa aagatatgca actacagcaa taattatata
    10561 actaaatatc tccactgaat ttcccccctt tagttatatt ttaagatatt tattttttat
    10621 ttttttgtct atactcaaac tttatggaag ttccttcaaa tccaaaattt tctcttatct
    10681 tattctcaag gtatctttga tatgagaagt gagttaaatc cttatcattt ataaacaatg
    10741 taattttagg aggtctaatg tcagtttgag tcccatagta aatttttaat cttctacctt
    10801 tgtcagatgg tggttgattt aacattacag cttctccaat tacttcattc agtgctgaag
    10861 ttgatattct cttagaatgt tcatttgaca catattcaac tgtatctagt atcttgttca
    10921 ttctttgatt agtttttgca gaaacaaata cgattggagc atacatcata aatggaaatt
    10981 tttcttttat atcttttgtg taattactca tagtcttatt atctttctct attaaatccc
    11041 atttattgat tacaaatatg catccttttc cttcatcatg tgctatacct gcaacctttg
    11101 tatcttgttc tgttacacct tctaaggcat ctattactat caatactaca tctgctctat
    11161 ctacagcact catagctctt attacactgt atttttctat tgtctcatat attttacttt
    11221 ttcttctaag ccctgctgta tctattagaa gaaatttctg tccatttttt tcaaaataag
    11281 tatcaattgc gtctctagta gttcctgcaa ttggacttac aattactctt tcttctccaa
    11341 gtatcttatt gagtatagaa ctttttcctg catttggttt acctgttata gctactctta
    11401 ttatatcttc ttcatactct gtatctaatc ctgctggaaa attttcaact atctcatcta
    11461 ataaatctcc aaatcccata ctattggctc ctgaaactgc aaaaggcgtt ccaaatccca
    11521 actcataaaa atcgtatata ttatcaaatt gactttgact atcaatctta ttaaccacta
    11581 agattactgg tttttttgtc tttctaagca attgtgcaat ttctttatca gcagctgtta
    11641 ttccagcttt accatcaact ataaaaagta ttacatgtga catgtccatt gcaagcatgg
    11701 cttgatttct catttgagat aatattatat cttcgctatc aggttcaatt cctcctgtat
    11761 ccactaatgt aaaatattta tctaaccatt ctacttcagc aaatattcta tctcttgtaa
    11821 ctccaggtgt attttcaact attgatatcc tttttcctgc aaatttatta aatattgtag
    11881 acttaccaac gtttggtctc ccaactacag ctacaactgg cctacttata ctcattttat
    11941 ttctccttcc atcttttgtt ttctatttta aaattttatc cacaaaatct tttccttcat
    12001 tcaaacaagg gataattttt attctcataa gttcttctaa ttgatttaat tcaatattat
    12061 ctaagaaaat ttcttcatct gcttttagca tacttcttgt tatataaaga gcttctccta
    12121 attctttatc ttcaagttgt tcttttaaat ccttagctgt taaaagacct gatacagtta
    12181 ttgtctctcc aaaaaacttg tttttgattt catatacatt tacttgaaga tttttaaact
    12241 tgtccatcat agcatctgcc atactctgta taaattcata tgcagaatga ccagtagcta
    12301 ttgatacttt cttctctctt tttagaatct tctcaggcaa tatatttaga tactcttcaa
    12361 tctctgtttt taacttacgt atcataccta caccatcttc aaactgtaaa aaaccttcat
    12421 attcttcata tccaggcaat tcactattag atagtatata aaattcatct gataaaaatg
    12481 caaatcgagt acctaatttt tcaagatatt tctgttgtat ttgatgtatt tgctctatag
    12541 ttttacttgc gctcttatca ttaaatatat ttaattctac tagattttct ctatgctttg
    12601 taatcccaac tggaactatc gcaacactgt ttacatacgg ataaagttga gctagttctt
    12661 ttattgttct atccaattcc tttccatcat ttatacctgg acataaaaca atttgacagt
    12721 tcatttctat atgagtatct gccaatctct tcattatacc atataatttt cctgcaaatt
    12781 tattgcttat cattttttgt cttaactctg gattagttgt atgcacagat atatttatag
    12841 gacttattct atattttata atattattta tatcttcttc actcatattt gtaagtgtga
    12901 caaaatttcc ttgtaaaaaa gataatctcg aatcatcatc tttaaaataa agagtttctc
    12961 tcataccttc tggcaattgg tctataaaac aaaacataca cttatttcta cagcttttag
    13021 cttggtcaat tatcggattt gtaaattcaa cacccaaatc ttcatcatag tctttttcta
    13081 tctcgtatat gtagacttcg ccatcttttt tttgtatttc tacatctaaa tattcatcac
    13141 ttagtaagaa tctatactct attatatcat gtattggctg ttcattcaca gatataagta
    13201 aatctcctac ctctatacct atttcttctg caatactatc tttataaacc ttacttataa
    13261 tattatttac tttttttaca tttacttcca atattttcac cactcttttg tattcatcaa
    13321 tgcttattat aatatatttt aatatgcaat ttatttattt attatatttt atgatagcat
    13381 ttttaagcat ttcatgtcat aataacacat tttaattcta atagtcaata taagtaaata
    13441 gctataaaat agattagaac aaataatatt caaatttaag tttcttaaaa atttgaatat
    13501 tatttgttct taaaaaaatt ttataaattt aatgttttac tatgattaac gtcccatttg
    13561 tcatatcatg aaccattcca cctacacact ttgacatcaa caatgcctta ttttgtaagt
    13621 ctttctcatc atttgcataa aatattggac aacctccacc atttttcata ttcttatcaa
    13681 gtgtaactat agctagggta tattcattta aacctatatc cattcccatc ttatgcactt
    13741 cctttctttc ctattttata attttttagt gataagtttt ttcctttaga acttgaaagt
    13801 attgggcatg actttacggc ttccattact ttttccacat tcttatctac tggtaaataa
    13861 gcaatcatta tactttcatc ctctttattc cttctaggta atggtacgaa tgaaggttct
    13921 ccataatctc catataaacc taaccttgaa tatatattgt atactattgc ctgtctttga
    13981 cctgagtcaa ataatatccc tgcattagca taatctttat ttttaggaat tatttgtatt
    14041 cctacacctc tattaatata tacttctcta tcacttttta gacctatatt tgttattccc
    14101 tttaaatctc ctaccttcaa tatagactca tctacaaaac taatctcagc aggcattaca
    14161 tcagctatgt ctccaatact ttttctagta agaagttttt ttaatataaa ggctaacact
    14221 aatccacaaa ttgaacttat taccaaactc attccttcac ttattttcgt ttctgcaagt
    14281 attacataat acaatcctat tgttaaaaaa gatgtaacta tacacatata atttcttact
    14341 tcataggttc tagctatctc ttctatgaat gcagctcctc tctttacaag ttgaacttcc
    14401 tctaagtttt gcaatgtatt tcttctattt gctctcactt gtctaaattg ctctgctgct
    14461 agggataaaa aagttataga tgtataagaa cgatttataa gagctggaac caataatgct
    14521 cctaatgaag aagcgacgaa tgctaatata agttgagata gtaaaacatt tggttgcgtt
    14581 ggatactgtt tttggtctaa attcaacatt ataattctag atgttatacc aataataaca
    14641 gcaacaaaaa atactctatc cattaaatca ctcctaaaat atttaatttg aaattttaca
    14701 tgtaaatcta caaagtcttt ctagaaaaat cactctgctt tccttttgat gtttctatga
    14761 taggtgtact tttcactaca tctatcatga catctatatc tttgagtatt ggcatatatg
    14821 gtataaccac agtacttttt tctaaattgg tcttagatat agcaacaata tcaacctcat
    14881 ctacatctct atctatcccc atatgaataa atagattatg tattatagct tgtctttgcc
    14941 ctatatcatt tactattcca aatgcaccta aatctcttgg tataacttct atggccagac
    15001 attcatttct atacttttct cttgtacttt ctagacctat gttagttatt attacaccat
    15061 ttaccattaa aataggtcct tcaaaactta tttttgcagg aactatatct gctatatctg
    15121 ctatactgtt ccttcttaaa aatcttctaa aaatcaaacc aacaatagct cctgacacaa
    15181 tagctaggat tgtgcaataa aaaaagctaa ggccatactt tcttgcaaaa acgatataaa
    15241 ctatcgaaga taccagtgca gaaaaaagac ttatataact tctagattca tacgtagatg
    15301 ctatttcttc aacgtaagcg tctccttttg gtactaattc ttcattatcg atattcttta
    15361 gtgttattct ttcttgttct gcaagacctt gaaactgttg tattgctact gcaaaaaatg
    15421 tcaaggctgc aaactcctta tctataagtg caggtaatgc tatagcacct aacgaagctg
    15481 aaagcccaga tataattatc tgttctaaat aatcttgtgg tcttgatggg tattgtttgt
    15541 ctgtaactct taaaactaat cctctacata atattcctac aactaatgca actataaaag
    15601 agtgtctaaa taaatttgca ttcaataaac tttcttccat tttctcagct ccttctctta
    15661 tatagtttgt gcaaaaaaca aaaaaaattc ccttctattt aaggaaattt ttttgtaata
    15721 ttgtaactat aaattcgcta ccaatgccaa gttcactttt tacttttata catccattta
    15781 gtgatttgac tatatgcttt gttatagcca atcctaaacc cgttcctcct acatccctac
    15841 ttcttgcttt atctacccta taaaatcgtt caaatatgcg ttctacatct tcttttggta
    15901 tccctacacc attgtctata actttaatta cgattttttc ttcatctttt aacactacaa
    15961 cttgtacaat cccattatct tgggtatact ttatagcatt atcaactagg tttaagaaca
    16021 cttgctttat atagtcttta ttggatagta tacttattga tttatcatca catttatata
    16081 aaacttctat atttttttca ctagctgaat gttgagttat ttcatatacc tctttaaata
    16141 catcatatac tacaacattt tccataagaa gagtatcttt attttctaca aatgataaca
    16201 gcaatatatc atcaatcaac ctttttaatc tatctgattc gctttctatt atttctaaga
    16261 atctatttct agtattcaca tctatatttt catttgactt tagtgtttca acaaacccac
    16321 ttatagatgt aagtggtgtt ttaagttcat gacttacatt tgcaacaaaa tcacttctca
    16381 tattttcaag tttaactttt ttagtaatat cttctatatt tactatcgaa cctattatta
    16441 cgttgttagt attctgaaga taaactgggt ctacttttat cttatatacg atatcatctt
    16501 tagtagtaat attagcagtt tcattatctt tagaaccaat aaattgaagt atttgtttta
    16561 atatttcttc ttcttttatt acaacattaa tattatgacc ttcaactaat tgatttggtt
    16621 cactctttat catttctttt gcttcatcat ttatcagaag tatatttcca tttacatcta
    16681 tagctaaaat tccatgagat atactcttta atatagacct tagctgtaaa tgtttatact
    16741 gtacttcttc taaggtttta tccatattct ttatcatatg attaaaattt ctagctaatt
    16801 cgccaagttc tcctttggct gatatgttaa gcctagcatg atattcatta ttacttactt
    16861 tttttgagac gtatataaac tcttttaaat atttccttaa tgttattgtg tatcttacag
    16921 ctaatataac aatggctatg gacaacataa caataaaaaa atatatacta tttaaatctt
    16981 ccacaagata acactcctaa aatataacct ttttaatcta ttttgtatcc tacacctctt
    17041 atagtctcta tatatttttc cgatttatct tcatcttcta ttttttttct taaatatctt
    17101 atatgaacat ctactgttct agtttctcca tagtattcat acccccatat tttatccaaa
    17161 agataatttc ttgaaagtac ttttccttta ttttgaatta ataattttaa caattcaaat
    17221 tccttaagag ttaactctat cttttcagag cctttggtaa cttcatgttt tgataaatct
    17281 agttttaaat tacctgtagt aagtatattg ttttcttcac ctaagctagt caaattatat
    17341 ctccttaaaa cagaactaat cctagctaac aactctttta tactaaatgg cttagttata
    17401 tagtcatcag cacctatttc aagcccttca accttgtcat tttccatatt tttagcagta
    17461 agcattataa caggaatatt ttttaagtcc ttatcacttc ttatttttct caaaacttct
    17521 attccactta tatttggtaa catccaatct aaaagtatta aatctggttt aatttcctta
    17581 gcctttataa atccgtcaaa tccatcatat gaataactaa cttcgtaatt tgacacctcc
    17641 aaattgaatt ttaatagctc aactatatgc atttcatcgt ctataacaag cactttagta
    17701 ttcattaaaa agctcctctc tattttatct gtagtaccgt tcctattcgt tctgatgcct
    17761 tattatgttt tctataagag tggaatttat ctgctctaca actagtgcat aaatttaaat
    17821 ttattatatt ttctttttta actccactac atctaagcat atactcatta attttccata
    17881 aatctaaata gtaacttccc tcttttatta tatagaactt ttcatcatta tttgtaagaa
    17941 ttgtgttaaa tttttctact aaatcttcag acacttcata acaacatggt ccaattgatg
    18001 gcccaattat acatactaaa tcttcaggaa ctgttttata gcattcagac attaatttta
    18061 ttgttttatg acctatatta ctaaaagtcc ctctccaacc tgcatgagcc agtcctatgg
    18121 ctttattttt agcatctata attgatatag gtacacagtc tgctgtaaac agtaaaagtg
    18181 gtactttttc caagtttgtt ataagtgcat ctccatctct tcttttccct atagtatctt
    18241 tattgactat attaactata tctgaatgta tttgagaatt actagttaga ttttcaaatg
    18301 tcaaatcaca ttctttacat acttttaaca tatcttctct attttttgca tctatattct
    18361 tacatgttat aactagattt acagaattta atttttcatc aagttcttca caattaccac
    18421 ttaaattaac atactcttta atatcttgta cattttttat atttatataa tctttcatca
    18481 tttgtctcct ttttctatca gtatactttt aagttcattt acaaaagtat ttatatcttt
    18541 aaattgtcta tacacagaag caaatcttac ataggcaacc tcatctaact ctttcaattt
    18601 atccattacc atctcaccga tttttttggt ttcaatttct tctataaaac atttatttat
    18661 ttcattttca atatgtacaa caatactttc aatctcgtca atagaaacag gtcttttttc
    18721 acatgctttt agcaatccat attttatctt ttctctatca aaatattctc tactattatc
    18781 ctttttaatg accattaaag gagttgtttc tattttctca tacgtagtaa acctcttttt
    18841 acatgattca cactcacgtc ttcttctgat agattttaaa tctgtatgtc tagaatctat
    18901 tacctttgat tctttataat tacaataagg acattgcatc tatattcctc ctttgtttaa
    18961 ctatatctca aactcatcta agcttaattc tgaacctata tttactatta ttgtatcaca
    19021 acctatttta agtatatcat tccaaaatat aacttcttca tcccctcctc ttgaaaaaaa
    19081 acttctactt ccatctccaa aaatggtaaa tgagactacc ttaccttcgt gaatatccat
    19141 gtctatgtct attataaacc ccatcctttt tccatttttt acatttataa tttctttttc
    19201 cattatatca gaaactctaa tcatattgta ctcacccttt ctttaaaaat aaatatgtat
    19261 cgaagtaaaa aaaaatgacc tatataggtc atttaaatta aaataatgtt tacagtccat
    19321 ggattactta tacatatttt ctcatattct ttaaagcatt tttttcaatt cttgatactt
    19381 gtgcttgaga tataccgatt tcatcagcaa cttctatctg agttcttcct ttgtaaaacc
    19441 ttaaatctaa taccaacttt tctctactat ttaatttctt aatagcttct ttaagtgata
    19501 tttcttgcaa ccaattttca tcagtatctt ttttatcttg aacctgatcc ataacgaata
    19561 ttgcatctcc attgtcttga tatactgggt caaacagaga tattgggtct tgtatggcat
    19621 caagtgccat tacaactgat tctacttcta attctaattc ctttgctatt tctgatactg
    19681 taggttcttt agaatttgtt ctaattaatc tttctctaac ttgtagtgcc ttatatgcta
    19741 tatcttttaa tgacctactt actctaattg ggttattgtc tcttagatac cttctaattt
    19801 ctcctataat cataggaaca gcataagttg aaaaccttac attttggctt aaatcaaagt
    19861 tatctatcgc ttttataaga ccaatacaac ctatttgaaa taaatcgtct atattttctc
    19921 ctctgttgtt aaactttttt attacactca agactaatct taaattccct cttacaaact
    19981 gttgtctggc ttcttcatca ccatttttta tttgtaataa aagttccttc atttgcttat
    20041 ttttaagaac tggaagttct gatgtattaa caccacatat ttcaacctta ttaacttgca
    20101 tatattttca gtcctctctc aaaagtattc atctattgaa attatttaca tttccaattt
    20161 ttttatactt tttaagaaga attaatttag cattatacaa attttttcat ttctttttgc
    20221 aaccttgaga ttattttctt ttcaagtcta gatatgtagg actgtgatat tccaagcatt
    20281 ccagcaactt ctttttgtgt tttttcaata cctttatcaa taagaccaaa tcttaattcc
    20341 ataatttgct tttctctgtc acttaatctg tcaagtgcca taaccaacaa atctctatct
    20401 atttcttctt caattatttt gtatatttca tcattttctg tacccaatac atcagataat
    20461 aaaagttcgt ttccatctaa atctatattt aaaggttcat caaatgatac ttctgttttc
    20521 tttttattat tttttcttaa gtacattaat atttcatttt ctatacatct cgaagcataa
    20581 gttgccaatt ttatattttt attcagttta aaagtattaa ctgcttttat caaacctata
    20641 gttcctattg aaatcaaatc ttctacatct atacctgtat tttcaaattt tctcgatatg
    20701 tagactacta atctaagatt tctttctatt aaaattgact ttacactttc atctgtttct
    20761 agcttttgta aaagctccat ttcttcttct ggtttcaatg gaggaggaag tatatttgcc
    20821 cctcccatat agtatattcc ctttggcttt attattttta atttaatacc taacatagta
    20881 ataaagctta ttattctttc tttcaatcgt aacattcctt gtccccctta aattttcacc
    20941 taaaatccag gcaatatatt tggattcatt attgctccat attctctatc attaaaattt
    21001 gaaataccaa gtattatatt tcctatcttt tgctcatcta cttctatata gtcagctttt
    21061 aagcctatta ttatgcttgt cttattactt ccagcatgtt tataaggtat tattctaact
    21121 ctacctgaag ttttatcatc taaacttcct ataatttctt gtgctttctt catatctata
    21181 tcttcataat taatatttaa tagtttttct ggaataaatc cttctaacac actagactta
    21241 actattatta catcactttg acttattggg tcttttaaca aattaccact atctactaaa
    21301 gccttacagc aaaaactttt ttctagtaag tttatattta tagtctttgt aagctcctta
    21361 atgtacttaa gcattttcaa atctttataa atgtatttta ataattcaca actcacataa
    21421 gtacatgcaa ttaagaaaga tatttttaag tgacttattc ctgtaaaata aattataaaa
    21481 taagtgcttc cagaaataaa aatgtttact agataaaaaa ctaacgctac atgaacataa
    21541 tcttttttgc tcttacttct aaatgaaatt aaagttatta tactcatgat tattatttta
    21601 aatggtacag taaatagtat attcattgat ggatacaaat aagcaacaga ataggcacct
    21661 cctaaactag cccccaataa cttatttttt ttaggattat atcttttagt aagaatagaa
    21721 gtacaactta taataatata atttattact aggttttcta taacatagta ctcaatatac
    21781 accatgtatc cccccttaaa acctttaaac atattataag atagtatgta caaaatttta
    21841 tgtcactttt gatggtcgaa tccaaaacaa attctattta aaaagaacaa tgatatatgt
    21901 cttatacttt atgtcgtcaa aagatatttt tttaatgcca aacaaataga cttttttgta
    21961 gaatccaata tatctaggta gaagttattc gctctacttt tggtatattt acaaaaaaag
    22021 actacataat ttaataatat atcaagtaaa tcttttactt atataaaatc aaattatata
    22081 gtctgtaaat tcaaatataa attattgtgc tagtaatcca tttgcatatt ttgcagattc
    22141 atttagtgct tgttctggag ttatgtctcc agctatcatc atttgaagtt gtttccataa
    22201 atattcatat atctctactc catgaggtcc aactgtcaat ggtctataca tatgtttatc
    22261 tcttgtcacc atttcttcaa atcttgggtc tccttggtat ttttcacctt tagcaactgg
    22321 agctctaggt atttctttgt ggaaagcttc cattgattct ttgcttagca tatactcaag
    22381 aagtttaaat ccaagttctt tatttttaca tgctgacatt aatgttaaat catctactga
    22441 agcaaaactt ccaactgtct tatcttctaa tgatgttaca tatccccagt ttaaatccgg
    22501 gaatgattta tcaaatgtac ttgtagcagc agaggatagc attatagtaa atgcagcttt
    22561 tcctggtcca aatacacttt caatcatttc attactatct tttgataaag catcttctgg
    22621 aatatacttt ttcatatcat ttataaagtt tgcagctttc aagccttctg cattattaaa
    22681 tttaactgat tttaaatcat cattatatat atctccacca gcttgccata aatacggata
    22741 ccagttccag tttaagcttc caaatacttt agaaccccaa ccttgtgcaa gaccccactg
    22801 gtcgattttt ccatctccat cagtatcttt tgttgctttt tcacagattc ttttaaaatc
    22861 atcccatgtt tttggaggtt tttctcctaa tttttctagt atatctttat tgtagtacaa
    22921 tacgcctgga ttagcagctt ctataggtaa accataaata ccacccatca tttttgcatc
    22981 atctaagtat agataattat ctgaagttcc tgatttttct aaataaggtg ttaaatcttc
    23041 taccgcaccc atttctataa attgaggaaa catctctgca tacatgtatc ctatatctgg
    23101 accttcacct gcacttattg ctgttgcata tttttctgcg tagttatccc atggtactat
    23161 ctgaaattca atttcacagt tattttcttt ttcaaatttt tcaaatatcg gcttccatgc
    23221 atctgcatca tggtctgtta atggtgcaga ccatacaact aatttttctt taccctcttt
    23281 gcctttagaa tcttcttttt ttgaacatcc tgcaagaatt gatactgata gaatccctac
    23341 cataactaaa gaaagaacct tttttagttt aatcatgctt aatccccttt cagactttaa
    23401 tttgtctttt tcctcaaatg tttacattta attcagattt aatattaaca aataagatta
    23461 ctcttgtcta atcataaaaa actatactaa ctcctataac tatatttttt ttctatacct
    23521 attagattta attcatacta aaaacttact ttttaaattt tatcaatatt taaaaatttt
    23581 tttaagtcta gccacaaact aactatctaa attgagcaaa ataaaaagaa gtctattgac
    23641 ttctttataa gcttacatat tatcttcttc ttcttaggaa tgtaggtatc tccatgctct
    23701 catcatcatc ttcttcaatt atacttgcct ttttaggagg ctctattttt tcctcagcaa
    23761 ttacttcttc ttcaatttct tttacagctt gttttactgt tgtatttaat gaagaacgta
    23821 tgcttgattt aggtttagta tctaaatcta aggcaccatt ttgtaatcct tcaaatcctg
    23881 tcgcgattac tgtaatcatt atttcatcac ctaaatcttc ttttatagaa gctccaaata
    23941 taacatttgc ttctgggtca caggattcca taactaaagt tgatgcctca ttaacttcaa
    24001 ataatcctaa gttaggaccg cctgtaacat taagaagtac tcctttagct ccttgtattg
    24061 atgtttctag aagtggagat tgaatagctt gtcttgcagc atcaattgct ctagtttcac
    24121 cactagcact acctataccc atatgagcaa gtcccttgtc tttcattata gtagtaacat
    24181 ctgcaaagtc caagtttatt aacccttcaa ctgctataag gtctgaaatt gattgtatac
    24241 cttgttttaa cacatcatct gcaactgcaa aagcatctaa cattgatgta tttttttgta
    24301 ctatctgtaa aagtctatca tttggtatcg ttataagagt atctactttt gatttaagtt
    24361 ctgctattcc accttcagcg ttcttcattc ttatcttacc ttcaaatgca aaaggtttgg
    24421 taactacacc tactgtaagt atccccattt cttttgccaa gcctgcaacc actggagcag
    24481 ctcctgtacc agtaccaccg cccattccgg ctgttacaaa gaccatatca gctccttgaa
    24541 gtaactttac tatttcatct ttactctcct cagcagctct tttccctact tctggatttg
    24601 ctcctgctcc aagtcctcta gttaattttt ctcctatttg aactttatat tcagcttttg
    24661 atgtatataa agcttgttta tctgtattta ctgatataaa ttcaacgcct tttagttgag
    24721 cttctaccat tctattgaca gcattgtttc caccgccacc tacacctatt actttaattt
    24781 gtgcacattc ttctaattct acgtcaaagt ttagcatcat aaaccccctt ctctcatgat
    24841 tcaaagttat cgtatgtatt aattatatca ttttcttctt ttattttgtt aacctttaag
    24901 taacatattc cacaaaaacg taaattttcc tttttaaatt tcttacttat attgtcaaat
    24961 attactcttt aagtataaaa taatttacgt atatattaaa tgctctttaa tttttacgtt
    25021 ttttgttcat aatattacta ataatatatc gtctaattat agataagtta ttaaacaatc
    25081 tcataccaaa aactaataca gcaacatagt aaagagaaac tcctaacttt tctcctatat
    25141 aagtcaataa tatagccaat atagcattac ctatgaaacc agtaataaaa attacattat
    25201 catagttatc ttcaaaattg gattttatcg ctccaaaaat agaatccatt attgcaagaa
    25261 tagctactga catatatatg gaataatcca tagaatatgt aaatggaata tagtaaccta
    25321 ctactacacc aaatgctaat cctcctaaaa cccataacat aaatcttcac ctctttattt
    25381 tactttgaaa cacaactaca ttacaatcct ttttattaat tttttttact ttttctttat
    25441 ttttttgtat ttttcgtttt attttgcaaa taaattcact ttatttccat tattttcctg
    25501 aacttttata ttaattccat atatatcctt aagagattta gcatatgatt ctggagagtt
    25561 tatagctgca ctcaataaat ctatatctcc tatagctttt attataaatg gttgtccata
    25621 tgtagtatca tttactgtaa tagtagctcc tgaacatttt atcttacttg ttgctagtac
    25681 tctttctcca tttatagaaa ttgctcttgc tccagccttt tttaaatcat ttaaaaccct
    25741 caaaatatct atatcatgta taatcaaatc gtttggattc tgtccgtctt ttaactctct
    25801 ctcactatcc ctcatagtaa tagttatgcc tggccctgta acagttgaat aaccacttaa
    25861 ttctttaact tcttcaagct ctttttgcat aagtgtttgg atagattcat ttccattctt
    25921 ttttgcttct ttatattcat tcaaagtttt tttgttcttt ttaagagttt cttttaagtt
    25981 ttttatttct ttttttgtat tttctacctc actttcaata ctttcagttt gacctaatgt
    26041 tatgtatact ctatttgggt ctagtgattt tatataagtt gatatgaaaa cgcctaatat
    26101 gaatgctcct aatataacta ttttcttttt catactatta tttcccctct gctttgttta
    26161 tatcactatg accatttatt tctatattgt cttctttggt cacttttact ttggcattat
    26221 aatatgtttc tatttcccag acaataccat actttatatt aagtgccgaa gccaatgtat
    26281 cagaatcacc aattgctttt attataattg gttcttttat gacagtgtca ttcatataaa
    26341 gcttgtcttc ttttaaatac atatatgtat tagaaactat tctctggtcg tttatagata
    26401 tggcacttgc ttgtgctgaa tttaatttat ttatcattga taataataaa tcataattat
    26461 atagtatact atcattttta tcagagtttc ctaagtcttt tgtagaattt atctcaattt
    26521 ttatgcctgt tccttcaaca tctgtatatc cagctaatgt ttcatatttt tctatttctg
    26581 acttaagtat gtcttgttct ccttcattgt ttttgcctct ataaatctct atatctttct
    26641 tcacactatt aacttctgat tgtagattct ctttttcttt ttttaaagat ttaagttctt
    26701 ctgtaagttg ctctcctctt ttaaaagttg tcatccctct attttctata ttaataattt
    26761 taagttgtaa actaacaaga acacccaata caatacttcc tatgattata cttttattta
    26821 taaatctttt tttcaatata tcacttcctt acaattgtct atccttctgg actatacact
    26881 gccaactttc catagttaag atttatagtc cctccattag tatttttatt ttgcaggtca
    26941 atcagaattt tacttaccct agatatatta taatctaagt tatcatcatt agaaagtaag
    27001 atttttatat tacttcttgt aagcatttct atattgcttt ctttttttaa gtttacataa
    27061 tttatttttc tataaattcc ttcttctttt aaataattta aagttttaaa tacattctcc
    27121 ttagttttat aatttttaaa ttttatagat ttattatcat caatgctata atctacatct
    27181 actatcaagt cttttttact ttcattagaa cctcttagtt cttccaataa atttcctttt
    27241 ttatctatat aacaataatt atcttcatct tttaatactg caaatatttc tttttctttt
    27301 aaacttataa ttatcttatt gggaagtttt ctttttattt ccacattttc tatatatgga
    27361 ttttttataa gtttattttt catatcttta aaattgtatg caaaaatatt ttcatttaga
    27421 tttacattta gttctttcat tatatttgat ttggtcaccc tcttatttcc aattacatct
    27481 attttcttta catcaaataa atcacttttt acaattatac atatacaaaa agataacata
    27541 agtataaaaa ctaaaactat catgacatta ttcgtattta actttcttct ttttttcaaa
    27601 tataatcaac ccctttggtt aataaataca cctttagagc ttgtttttcc tacaaaaaaa
    27661 ttataatatt taaagtcaaa tttttgcatt ttattacata acatccaaat aaggtattaa
    27721 attttataaa taaatcaata tttcatcaaa ataaacatag catactacat actatttata
    27781 tatttaaatc tgtatacata caaataaacc cctaatttat aataatttag aaaatctaat
    27841 atattataaa ttaggggtaa atatttactt atcttttctt tattcctatt accttagctt
    27901 gaccttctat acttggagtc gtttcttttt ttatttcttt tacttcctta ataacttttt
    27961 ctttttttgt ttttttagaa gttgactttt gtgttgaatt gtatactttc attatctcat
    28021 catatataag gtctatggct tcaggcttac ctatagtttt acttgcattt gccatatcaa
    28081 ccaaaagttc tctatctcct aaaagtttaa atactgctgt atttaggctc tctggagtta
    28141 gatttttttc taaaatagca attcctgccc cttgtttttc tatactcttg gcattatact
    28201 cttgatgatt ttcagcagta taagctttag gtattattat agatggcttt ccaagagccg
    28261 ttatctcagc taatgaaatt gctcctgcgc tacctataac caaatcactt gctgcaagtg
    28321 catttgccat atcttctaag taaggcacaa ctttttggta tggtttaaga tttatatcgc
    28381 taatacttcc cataaattca tcataatagc ttttgcctgt agcaaatata aaagctatat
    28441 cttcatttac catattcttt attacaagtc tcattgcatc atttattttt cttgaacctc
    28501 cactaccacc atagcaaagt accattcttt tttcgtcact tattgataga ttctttcttg
    28561 caatattttt tcttgataat aaaatttctt ttctaactgg atttcctgta aaaactaatt
    28621 tatcttctgc tgcctctgga aatcttttat gcgaatcttc aaaacttgta agtacctttg
    28681 taactgtttt agataaaatc ttattagtta caccaggaaa agaattttgc tcatgtatta
    28741 tggctggtat tttccccata gatgcattaa ataaaacagg tccactaaca tatccaccag
    28801 ttcctataac tatatctggt ttaaatttct taacaatctt tcttgattgc tccaaacctt
    28861 taaaaagctt aaaaactctt tttacattat cgaaatctat ttttcgctta aagccttgaa
    28921 ctgttacagt ctttaattca aatccatact taggaactat ttctgactct attccctttt
    28981 ccgtcccaac aaaaattatc tcagcgtctg gatgctcatc tcttattttg tttgcaatag
    29041 ctatagctgg atacacgtgc cctccagttc caccgcctga taataaaact ttcatcaaat
    29101 catcatctcc tgttaattaa ttttgacatg ctttgagatg tttaacacta ttcctacagc
    29161 tcccataaag atggtgagtg aagttcctcc ataacttata aatggtagtg ctacacctgt
    29221 agctggcatt gatgatgtcg caactgctat atttaaagct gcttgtattc caatttgtgc
    29281 acctatacct ataacaacca tacaagcaaa tacattagaa gtcttcaaag ctattcttac
    29341 acatctgtag actaaaacta caaatagcat aataactatt atacaaccaa tcaatcctaa
    29401 ctcttctcct attatagcaa atataaagtc attttgtggc tctggtatat aaaaatactt
    29461 ttgtttactc tttcctaagc ctagaccaaa caatccacct gaacctagtg catatagacc
    29521 ttgtattacc tgatacccct taccaagagg gtcttgaaaa gggtctaaaa atgatgtaac
    29581 acgactaagt ctatagggtt cagcaattac taatgcagca aataaagcag ccccactccc
    29641 tatcatagca aatacaattt tcatatccat tccagcaaca aatatcatta caaaagtaac
    29701 tagaattaca gtacctgctg ttgacaaatt tggctgaagt attatcaacg caaaaaatat
    29761 ccctggaact acgagaagtg gtacaacacc ttttgtcaaa gattttattt tatcataatt
    29821 tttttcaatt aattttgcag ttaatattat tgtagcaaac tttgcgattt ctgcaggttg
    29881 aaatgttaaa gctcctattc ccaaccatct ttttgctccg tttgcttcta tccctaaagg
    29941 ggtcaaaact aacaataata atactactgc tatagctcca attgctgtag catttttctt
    30001 ccaaaaacta taatctattc ttgatgtaat aatcataact ataaatccaa gtacagcata
    30061 tattacattc ttttttagaa aatagtacgc atcattgtgt ttaaatgaag attgtataaa
    30121 acttgcacta aacaccataa ctattcctac aaatactaaa agcatagtag tgtaaaacac
    30181 tactccatca aattcagtct tcatccttat gtcaatttgt tttttcaaat tttctttagg
    30241 catccaacta atctacctcc attcttccat ttaagtaagt ttagaggtaa gagattattt
    30301 taaattgttt acattatctt tgaagtcttt tcctcttact tcaaaacttt catacatatc
    30361 ccaacttgca caagctggtg aaagaagcac tatatctcca ctttttgaaa tttcataaga
    30421 agtcttaact gcttcttcca tatcttttac tatatgtatg tcattaaatc ctttattttt
    30481 agcacaattt tctatatttg atgcagtttc tcctaataag accaatgatt taacatatga
    30541 ctttgcagtt tcaagaagtt catcaaaatt acttccctta tccatccccc ctgctataag
    30601 tattataggt ctatcataag actgtatagc ttttatagtg gaatctggat tagttccctt
    30661 tgaatcattt acatatatta cattgtctaa atttcttaca aactcttgtc tgtgttccac
    30721 tcctttaaat gtcataagta catcctttat aacttctaaa tcaattttgc agacatatgc
    30781 tattgctata gctgccatac aattttctaa gttatgacct ccaggtaaac ttacatcatc
    30841 tttatttaga aatttgattt tatcatctat atctattatt atgtcattat tttctaaata
    30901 tactcctcca tttacttttt tagttcttga aaagtaaatt actttggcat tgcatttatc
    30961 agctaaagat tttactatct catcatcata gtttaataca cagaaatcct caacagtttg
    31021 gttcataaat atatttgcct ttgcttctat gtatttttcc atagtatggt gtctatttaa
    31081 atggtcctct gtaatattta atattgcgct tactttaggt ctaaattcat ctatactctc
    31141 aagctgaaaa ctgcttagtt cagttactaa cacagactct tcacttgaag tttctatagt
    31201 atctattaca ggatttccta tattaccaac tacatatgta tctcgttttg ctcttgaaaa
    31261 aatttctcct accaaactag ttgtagttgt ctttccattt gtacctgtaa tccctataaa
    31321 aattggatta ttcgatagtt taaaagctaa ttctacctca ccaattatat acttgcctga
    31381 attttttaat ttcaatatga atggtaggtc tagtggtaca cctggagaca ctacaaccat
    31441 atctatatca tctacatctt ctgggtgatg tcctaatatg tattttatgt catttataga
    31501 tttcaattca tcaagtatat ttctcaattt attttcatct tttatatcat ttacgattac
    31561 acttgcaccc aatttatcta aatgtttaat tgtagatata ccagtttttg caagtcctac
    31621 taataaaact ttttttcctt ttaagtccat ttttcattcc ccctagccct aaaatactgc
    31681 tattatactt atccaagcta aaacaactgt tataatccaa aaaataaata caactcttgt
    31741 ttctggccaa ccacactgtt caaagtggtg gtgtattgga gccatcttaa agaatctctt
    31801 tcttgttgcc ttaaaatatc caacttgaat caaaactgat agagcctccg caaaataaat
    31861 tcctccaatt ataggtatta taagcactga gttagttaga actgaaaatg ctacaactgc
    31921 acctcctaat gccattgaac ctgtgtcgcc cataaacact ctagccggat atgaattaaa
    31981 tcctaagaaa cctaaacatg ctccaacagt tgctgctgct aaaactgcga cttctgtatt
    32041 tccagcaata gaacttgcaa atagcataaa gaatacagaa actattaatg ttactcctga
    32101 tgctaatcca tctaatccat cagtcaagtt tacagcattt actattgcta cgattataaa
    32161 catcattatt ggtatatata atattccaac atttatcaca aaatctgtaa atggtatcat
    32221 aagttgtgaa gcacttgatg atgatgtata ctgataaaaa gcaacataaa aagataatgc
    32281 aacttgaagg attatttttt gatatgcttt tagtccaagt gatcttttta gctttatttt
    32341 tataaaatca tctaaaaagc caataaaacc aaaacctgca atacatataa gacctactgc
    32401 catatcatga ctaactttta ctcttgtaag tccagttatt agaattgcaa caatcattat
    32461 aattccaccc atagtaggtg ttccgttttt agctagatgt gtttggggtc catcatctct
    32521 aactgtctga ccaaatttaa attttctaag cattggtata aatattggtc ctattataat
    32581 tactattaaa aacgcaatca atgcggtgta cgtaagctct gttattccta acatcataaa
    32641 actcctctcc cttgtcaatt aactcattag tttaagctta tttatttaga tattcgacta
    32701 ttttttctaa tctcatgcct cttgagcctt ttactaaaat agtgtctcct gtttttacta
    32761 attcatttaa cttgttaaac acatcttctc tattctcaaa gtgatatata ttggctgaat
    32821 tgaatcctaa ttgttttgct tcttctccaa taaacactga attttcacct atagttatta
    32881 taatatcagt attattcata gatgattttc caactaatct atgaccatac tctgaaattt
    32941 cacccatttc tagtatatct ccaagtatcg ctacccttct acctttatat cttccaagta
    33001 tctttaaagc agcatccata gaatctggac tagcattata tgaatcatta attattgtta
    33061 atttttcatt ttttattatg tctaatctca tttttgtccc tttaaaattt tttaaacctt
    33121 tttttatata atctaaagaa atatttagac acaaaccaac taatatagca gacattgcat
    33181 tatatatatt atgttctcct attgttggta taaatatttc ctcttttttc ccatttatta
    33241 cacatataaa tgttaaacta tcctcttcca tagtataact ttcacaataa acatcattgt
    33301 ttttattaaa gccaaatgtt tttaacttat atacatgttc tttttctttt aatgtagata
    33361 aaaacttatc atctccattt acaattaaag tatttgtttc atcaaagttt gaagtaatct
    33421 ccatctttgc tttcaaaata ccctcttgtg aacctagttt ttctacatgt gataatccta
    33481 tattcgatat gactgcaatt ttaggattta ctatatccac taaatatttt atttcattga
    33541 atccagacat acccatctct attacagcac actcatgttc tttatttaga ttaaataaag
    33601 tcaatggaac tccaaaatga ttatttaagt ttttttcatt ttttagtata tttaactttg
    33661 cagatatggc agagtaaatc atatctctag tagtagtttt tcccacacta cctgtaacac
    33721 ctataaatgg aatatcaaat ttttctttat agtattttga aatatctcct agagccaaag
    33781 atgtatcttt tacctctatt aaatttatat ccgaactttc taattttatt ccattacttt
    33841 cattttttat aaaagtttta catccactat catatgcaga cttcataaat gtatgcccat
    33901 ctaaattttc tccaactatg gctacaaatg cattttgggc atttgcctcc ctgctatcta
    33961 taactatatc acttacacag tcattataat ctccatatat taacttccca ttagttgcaa
    34021 gtactaactc ttttattgtt aagcactcca ttattaaact cctcctttaa aaccttagct
    34081 atattctctt agcaacaaaa ggcaatgtat accgttatat atacattgcc tacgataact
    34141 cttaacttta taattatata ctatatttct gtttttttga agatgtttat gggtatatgt
    34201 taattatctg ttacttttta gttttcaaaa tatagggtta tacttgagtc ttcattaact
    34261 tttactccag gttttgggaa tatatccttt acctttgtat cttctggaag ttctatatct
    34321 gcatctaagt ctggttttaa cttcgcatcc tccaatgcct ttacagcatc tcctattttt
    34381 agatttctta catctggcac tttaacttgc ttcttttcat actcagcttt ttcttcttct
    34441 ttatatactg gtttaactcc caaatatttt aaagagtcat tcattatttc tttcactatt
    34501 ggtcctgctg tagtactacc aaatgcactt actcctgttg gttcgtcaac aatagcaaga
    34561 actactattt gcgggtcatc acaaggtgct attccaacaa atgaacatat atattttcct
    34621 ggagcatact taccatctat aactttttga gctgtacctg ttttaccacc aagtctataa
    34681 cctggtatat atgctatctt tcctccacct tcagttacaa ctgattctgc aatttctaac
    34741 atttttttag atgtttcttt agatataaca cttcttactt tttttggttt tactgtttca
    34801 gttatatttc ctttattgtc agtataagat tttaccactc ttggttgcat taaatctcca
    34861 ccattagcta ttgatgatat agctgttata agttgtattg gagttactga tatagattga
    34921 ccaaatgata ttgtagccaa ttcaactggt cctacatttt tttcgttata cagtattccc
    34981 ttagcttctc ctggtaaatc tatacctgtc ttatccataa gaccaaatga ttctatataa
    35041 tcatacattt ttccaacacc taatctgctt cccaactcaa caaatactgg gttgcaagag
    35101 ttttgtactg cttgtttaaa ttcttgtgtt ccatgaggtc tgtaatgtct ccaacattta
    35161 atctttcttc ctccaacagt tacactacct gtacatgtga atttttctcc atctttaatt
    35221 actccttctt caagagcact ggaagatgta ataagcttaa atgtagaacc tggttcatat
    35281 gtatcactta ctgctggatt tctccacatt tgataatagc cttttatttt atctttatca
    35341 ttatattttt caagttcttc ttgataatat ggatatattg gcgttcttga gtcattaggg
    35401 tcataatctg gttttgatgc taaagcaagt atatcacctg ttttagggtt catagctatt
    35461 gcagtaacct tttttgcatt atttaactca taagctttct gtactgcttt ttcagtatag
    35521 tgttgaatta cttcatctat agacaacaca agaccattac cttgtacagg ctcataatat
    35581 ttttccattc cttgtggtat ttctcttcct gaagcatcag tacttactat aagttttcca
    35641 ggctttcctt ttaacttttt atcatattgc atttctacac cagatatacc tgttgcatct
    35701 gaagaagtat gtcctaacac atatggtgca aaattcccat atgggtaata tctttgatta
    35761 tcttctgcca cccatattcc acttaattta gcatctctta tttgacttgc cttatcatca
    35821 tctatccatc tttttacttt tacaagagcc atattttttt tgcttattaa agcgtaaata
    35881 tctttataat cttcatccaa aatctcagct accttttcgg ctgcttcttt tttatcttct
    35941 acttccacag gtttacacca aacagtatac ttagttacac ttactgctaa ctctttcata
    36001 tttctatcat atatagttcc tcttttgggt tctattggta tatctcttgt ttgttgttct
    36061 agtgcttttg tactcaacca atttcccttc ataagttgta gatacccagt cctaataaca
    36121 aggcaaaaaa acaatgcaca tgctagaata agaacgagta ccagcctttt cttacttatc
    36181 ctctttactt ttctcaaatc tattccccct agttatccac atctatatac tttatttgtc
    36241 catcttttgg atagtccata ttgagcaatt ccttagcctt tagctcaatt tcttttattg
    36301 atgttttttc agtttctttt gcctttagtt cctctaatac tatctctttt tttcttaaat
    36361 ctttttgtaa ataatagata tcgtacttca tttccgagat ttttgaataa ccacatagat
    36421 tcaatattat tactgaaaaa gttgcaatca gtaaaatgca tttagctcct atttttttct
    36481 tatttctctt tttataatta ttcttttttt tatttttata ttcatttaga ttatgtattt
    36541 tataattttt tttcaaattc tcaccccgat agtatatagt ttaaattttc ctctatacta
    36601 tatttgttat taatcatatt tcttcctcta aaatttcatc gctaaattaa tacactatta
    36661 cacaatctaa ttgtattttt ttctatacac aacaaaaacc tataaattca cttatacttt
    36721 tatatgaatc tataggttag atattaattt ctatttattt ttatcacttc tagtattttt
    36781 agataatttc ttccataaat ataatatgta actaactatc tactctctga ttcctaagtt
    36841 ctccattata ttacttaata ccatctatat ctatattttt atatctaaat tttcattata
    36901 tatctcaata acaaagaaca tttcttatag tgttaattgt tttttaattg ctttaccagt
    36961 gcaacaaaat atataatact ttttaattaa ttataataat cttattttat atataagatt
    37021 attctttagc cttataacta cttatatttt ttctgctact cttagcttag cacttcttga
    37081 acgtggattt acttctattt cttcctcact aggtaatata ggttttctag ttattatttt
    37141 cacctcagat tctttatcac attggcatat aggtaaatgt tgtggacata tacaatctga
    37201 tgctaaatgc ttaaatgcat ttttcactat tctatcttct aatgaatgga atgttattat
    37261 acatattctt ccaccttcat tcattataga agaagcatca tttatcatct ttgtaattac
    37321 tccaagttca ttatttactt ctattcgtat tgcctgaaaa gttcttttag ctgggtgagg
    37381 tccatctatt ctagctttct tagggatagc ttttttaatt acatctacta attctcctgt
    37441 agtttctatt ggtttgtttg ctctttcttc aactataaat ttagctatac gcttagccca
    37501 gttatcttct ccataatctt ttattatctt agttaattca tcttctgtat aagtatttac
    37561 aacatcatat gcagaaaact cacacctaac atccattctc atatcaagtg gtgcatcttg
    37621 catataagaa aatcctctat cagcttcatc aagttggtga gatgaaactc ctaaatcagc
    37681 taatacacca tcaattttgt aaactcctat tttttctaat tcctctttta tattttcaaa
    37741 attgctgtga acaaacttca ctctactttc atattcactc aatctctctt ttgctgtgct
    37801 tatagcattt ttatcttggt caaaccctat aaataatccc ttatctgaca gtttttttac
    37861 tatttcttta gaatgtcctg ctcctcccat agtacagtca acatacacgc catctggctt
    37921 tatatttaaa ttctctatac actcatttaa aagaactgat acatgatgaa attccatagt
    37981 tacctccctt aaattcactt tttaaactat tcttcactaa tattattttt atttttcttt
    38041 gataaataaa catgtgcaat tatgccacca ataactccaa ttagacttat aacttgtgcc
    38101 attctaagtg gtcctaacat taaactatct gttcttaatc cctctataaa aaatcttcct
    38161 attgaatata aagttatata agtaactata acttgacctt catatttttt attttttcta
    38221 aatagtaata aaactataaa tattccaaaa tcccatatag actcatataa aaaagttgga
    38281 tgtaccttta ctccatcaac cattattccc caaggtaaat tggtagcacc tccatgtgct
    38341 tctccattaa taaaatttcc ccatcttcca atagcttgtg ctaaaggcat tccaagtata
    38401 actgtatcag ccatttttaa gaagttaata tttttaatct tagtgtatat ataaccagct
    38461 aatattccac caattaaccc tccatgtata gcaagtccac cacctctaaa attaaaaatt
    38521 tgagacatat tttgagcata gtaactccaa ttaaatacaa cataatagat tcttgcacag
    38581 attagtccaa ctggaattgc tattatagct atatttaata catcatcttc tttaattccc
    38641 actctttttg cttcttttat tgctattaaa gttcctaaaa tcattccaca tgccataagt
    38701 attccatacc acatgatgtc tatgccaaac agtgtaaatg ctactctatc cataacgtca
    38761 cctctttgta ttaattgaaa atatttccaa agcaatatgc tataattaca attaattgta
    38821 attatactaa attatgataa cacattaatg ttaattctac aaaaaatatt aaaatttatt
    38881 ctacaactcc ctacactata ttatatctaa ttactataaa attttttaaa ctgaatctct
    38941 catgactaaa gttacaagtg tacatagata ttttttaatc aaaaagattg atgcatatat
    39001 acatcaacct cttctaattc ttatctatag ctcttaaatt tttacctcaa tttatataaa
    39061 tatattttat aaatttgttt taccaaaata aacatttaaa aacataaact aatacttaat
    39121 tagttattct tattatgatt aagtttctat ttaggctcta caatagaaat aacacaatac
    39181 tcttctttaa aatgctcttt taatctttct tcaacttcct caaaagtaat atctttaata
    39241 acatctaaat actccaatag atttatatcc ttaaaaacat aagatataaa actattacct
    39301 ataaagttta tagaatcaaa gcacttaata aatgacccta tttttttctt tttagttctc
    39361 tcaaactctt cttttgataa tccttctttt tttgattttt ctatataatc aagaattatt
    39421 tcttttactt ttttaggctc tttagaatct ccagctatta tgctaaaagc ataatccact
    39481 tgagacgaaa atccagctcc aaaattttca tttattaaac cttgcatata caaatcttca
    39541 taaagcttac ttcctttttt aaataacatc cctacaagta tatcagttac aatctcttta
    39601 cgtaaaagtt ctttcccttt taaaccaaca ttactatctt taaaacctat gttgaacata
    39661 ggcatagata tagggaattt ttctataacc tctttttctt ttacaccttc tggttcttct
    39721 ggataaaatc tttctatact cttagagagt ttatctactt tgtaattgtt tgacttttta
    39781 gttacatcta taactttttc tacatctaag tcaccaacaa caaataaagc catatttcct
    39841 ggattataga aagtattata acatttatat agctcttcct tagttatctt atatatacta
    39901 tcaacagtac ctgctatatc aattctagca ggataattta catacattgc cttcaagcaa
    39961 ttaaagtata cattccaatc agggtcatca ttgtacattt ttatctcttg tgctattata
    40021 cctttttcct tttcaacatt ttcatctgta aaataaggtg tttgaacata gtcaattaga
    40081 tgttctaaac tctcataaaa attttcagta gcagaaaata agtaggctgt cattgtaaaa
    40141 ttagtaaatg catttgcatt tactcctaat tttgaaaatt tatcaaatgc atctcctcca
    40201 tcaggttgct caaacatctt atgctctaga aaatgtgcaa taccttcatt cactctaatt
    40261 ttcttatctt ctccaattgg tacaaattct aagtcatttg aaccataatt agtagctaaa
    40321 atagcatact ttttcataaa acccctttta ggcataaaat atacatctaa tccattttgc
    40381 aatttctcat aataaacttc ttctttcaat atatcattaa ctattttttc catagacatc
    40441 tcctccatta atttctcaag aagtatacag tatcaagctc tatatttttt atagcttcaa
    40501 ctatatcttt ttttgtaacc ttttctatac tagatattat atcttgtact tcagaatttg
    40561 tctttgccat agattgagag aaaacaaaat cagacatacc acctatatta tcagttatag
    40621 atttcataga attaactaaa gcaagtttac tgttttctaa ttcttcatca gatatatcac
    40681 ctgcttttac actttctact tgttttttta tcaattctac agctttatca taatctttag
    40741 tttctatacc agatgatata aacatgatac ttttatactt ctctattgaa gagaatatat
    40801 agtaacataa actttctttt tctcttacat taacaaataa tttagagtgt ggaccaccac
    40861 ctaagacatt gctcccaact actaaagcat aatatttatc tatgtctgca taatccacat
    40921 ttgctctata acccataact aacttacctt gagttatttc catttgttca tctataactt
    40981 ttacctcatc cacatttttt ataaaatctg ctcttggtat atttataatt tcttctcttt
    41041 caaattttag atttttactt attatatcta caactttatc ttcatcaaaa ttaccttcta
    41101 caactatatc tataggtgat gttttaagta tgtttttata atgttcatat aactctcttg
    41161 aagttatact atctatttca tcttcataac caaattcact tacactatat ctctctcctt
    41221 tacacatagc ctcaatacat ttatcttgcg catattcctt tttatcattt attattgatt
    41281 gtatcttttc tcttaaattc tctttttcaa tatttaaata ttcttcttta aacccaccat
    41341 ctacaacaag cgtattattt attacttcat taaaaaattc tactacttct tcaaatatac
    41401 tctcatctaa atacttttca cttatgttaa ttactttaaa actcaatact tgacgttctc
    41461 cacgttttac agcatcagct cccatagatg aaccgtataa atcatctaat ttattggata
    41521 ttgctctagc actaggatat ttagagcttc cacttgctat tattgatggt attagagcat
    41581 tttttgtagt ttctttttca tccaatagtc tctgaacata taaacttata agattcgatt
    41641 taaacttttc tgtttttatc aaagtaagat taatattatt acctaaattt attatttttt
    41701 tagtattaac catatgtacc tcctattttt atttaaacat caattttaaa attgaaattt
    41761 atttattttt taattacata tactcacata taaaaacctt ctatttaatt atatcctttt
    41821 tattattttc taaaagcaat atttttattt aaattaaata caaatattat ttaaatcaat
    41881 atgctttaaa gtctagttta caagcttgat atatatgatg tataatcaat atgtatgtct
    41941 ataattaaaa caattttgca atcctataga ttgattgttt aaagttatat aaagaatata
    42001 aaaaagaata agagagagga cgattaatat gaaattaggt atagttggtt taccaaatgt
    42061 aggtaaaagc acactattta acgcaataac tcaagcaggt gcagaatctg caaattaccc
    42121 tttttgtaca atagacccaa atgtgggagt tgtatctgtt ccagatgaaa gattaaacaa
    42181 actacaagaa ttatataatt ctgaaaaaat agttcctact gctatagaat tttgtgatat
    42241 agcaggttta gttagaggag cttctaaagg agaaggttta ggaaataaat tcttatcaca
    42301 tataagagaa gttgatgcaa tagttcatgt tgttagatgt tttgaggatg aaaatgttgt
    42361 acatgtagat ggttctgtag acccacttcg tgatatagaa actataaact tagaattaat
    42421 tttctcagat atagagattc ttgaaagaag aattaataaa actcaaaaag ctgctaaggc
    42481 tgataaaact ttaggttctg aacttgattt attaaaatca ataatgtcta ctttagaaga
    42541 aagtaagtgt gtaagaacta tggaatttac agaagatgaa caaacattcg taaattcatt
    42601 ggatttactt acatctaagc ctgtaatata tgcttcaaat gtatcagaag atgatttagc
    42661 tgataatgga gaaaataata aatatgttca acaagttaaa gcatttgcag aaacagaaga
    42721 tgccgaagtt gttgtagtat gtgcacaaat agaagcagaa atttctgaac ttgattctgc
    42781 tgaagaaaag aaagagtttt tagaaacatt aggtcttgaa caatcagggc ttgataagct
    42841 tataaaatct tcttatgctt tacttggttt aatttcattc ttaactgctg gtccaaaaga
    42901 agttagagca tggactataa aagttggaag taaagctcct caggcaggag gaaaaataca
    42961 ttctgatata gaaagaggat ttatcagagc agaaacaata gcctttaatg atttggttga
    43021 gcatggtact atggctgctg ctaaagaaaa agggttggtt aaattagaag gtaaagaata
    43081 catagttaaa gatggagatg taatattatt tagatttaat gtttaaaatg attaatcgta
    43141 tacactttga ctattatata taataa
//
LOCUS       DAMJWU010000025        40014 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMJWU010000025 DAMJWU010000000
VERSION     DAMJWU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.025"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            280..735
                     /gene="smpB"
                     /locus_tag="PC460_001476"
     CDS             280..735
                     /gene="smpB"
                     /locus_tag="PC460_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF2600368.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            893..1242
                     /gene="ssrA"
                     /locus_tag="PC460_001477"
     tmRNA           893..1242
                     /gene="ssrA"
                     /locus_tag="PC460_001477"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1357..3384
                     /locus_tag="PC460_001478"
     CDS             1357..3384
                     /locus_tag="PC460_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2600369.1"
                     /translation="MSIDNNFNHVAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIIKSVYEEYDFLPEEERDELVIVDLKENHDINLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNNVKEIIGKFKFKDEINQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNKMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEILFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKINPSAIVNVYGQELLDESFAICQADMLMKGQNPDNIRLGNT
                     LTQDRFNGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIENDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEAKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEQIKEI
                     YEKFEESENSKIFDNEDFGYRKVTIERPLKLSFKVNEEAIENVKNTTQFINLAVSKKK
                     DIEVKEKEEAEGKIKQDKLIKLLESFDNTLEYMDRDKFIKDLKAKSKEFDIQLSAGLI
                     KAIVNTIGVRNEDAEVCKDSKGNIESDSSLKDTESIGLKDDVYEYFEKEVKPHVNDAY
                     IDESSINNIGYEIPFTRHFYKYEELRSFAEIMKEVESLESEIALKIRKVLG"
     gene            3384..4775
                     /locus_tag="PC460_001479"
     CDS             3384..4775
                     /locus_tag="PC460_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2600370.1"
                     /translation="MGYRYRSDDELKDSGVEWLGKIPKDWEVRQLKRVLSDRKENNEP
                     IKTSNILSLTIEKGVIPYSEKGTGGNKAKEDLTQYKLVYPNDIVLNSMNVIVGAVGLS
                     KYFGCASPAYYMLYKINANDDIRFYNYIFKSKLFQDSLKGLGNGIMMKQSETSGKLNT
                     IRMRIPMEKLSSVKIPIAFNLDQEKIANFLDEKTSQFDSIISKKEKLIERLEEAKKSL
                     ISEVVTGKVKVVKTDDGYELIKRSSEEMKDSGVEWLGEIPKEWDIKRLRFLGKLQNGI
                     SKSSDSFGYGYPFVSYSNVYRNIELPHIVEGLVNSTEEERKIYSVEEGDVFFTRTSET
                     VEEIGFASTCMKTIDNSTFAGFLIRFRPNDKRLYKGFSKYYFRCDLNRKYFVKEMNLV
                     TRASLSQNLLNNLSVVLPKYEEQVEIYNALELKVSYINLSINKLKYQIEKLKEAKQSL
                     ISEAVTGKIEILD"
     gene            4837..6108
                     /locus_tag="PC460_001480"
     CDS             4837..6108
                     /locus_tag="PC460_001480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600371.1"
                     /translation="MDKNIFQININTDVLIRLRNQINEEYYISYRKEFYDKNNKKHRA
                     WDKICAIMDRLDDTVDYLNKLKLNTGKYRRSAFDFFEFMNQASVVMDCITHLAKIFEV
                     PYEEVETSTEIFNELGKEGKGTDKKYFEYLRSLCSVHPMETSRHKIYQENSFECSPYV
                     VWNDKSIWTNDASDLYAVVYTSKDNDSFKRIHIYMNQIFEYVKRRVNFIENIIEHIEK
                     YQQEIIEIFRNTPIKKEEDFESYIDYLRNLKKEIEIRYGNDSFHELNYLIELFDLPLS
                     NPDNEEKMNLYKNALKYAIKFEHNSIQNMTNEGYKNNGILYPQVNITTTLLEELISLN
                     SGSEERRKYRYAIEKTSYLSYDSCWSDREWAYDQIDDMMPFLERYVTFDKAQSDFEHY
                     VLVKLALYLECLENECLINKNVPNDKKFRIK"
     gene            6143..9124
                     /locus_tag="PC460_001481"
     CDS             6143..9124
                     /locus_tag="PC460_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDF2600372.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVDDLFRFL
                     ENTQKELLDEFKYNRGSNYKKAFIDLLNSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKKDRNPKETLFGFNQRVLVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     RGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKGNQRAIFPRFHQLDAV
                     RKIVEDLKINKVGKNYLIQHSAGSGKTNTIAWTSHRLSSLHDEENNAIFDSIIVVTDR
                     KVLDKQLQNAIYQLEHKNGLVVKIDEDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLDKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKVIDAENEKNEKSSIDKMN
                     ELIEKRTNLGNISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPISVYYKVGKKIIDNPEFDKGEAKRAINKFVSLNEHNIRQKVETIVDDFVDNR
                     SMWLEGKAKGMIVTASRLHAVRYKLAIDQYIKEKGYDMKALVAFSGTVKDGDDEYTEV
                     GMNKEVAPDITDSNLPNIFDKNEFKLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKQTFILDFQNSVEDIQDAYKPYFEMTNIDKVTDPNVVNDLWYQLHEY
                     AVYTDEEVNDFTVLFYKEKRTSSQDAKMNNLIDKAVERYCYLEEDDEKKSDEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLKKLPKKSKQRIDLDDMLSLDYYKLQKL
                     GLDDKKGTDISLYNGEGELVGISETATSRVSEADEEYLEDIIKKINDVFGVGLTEDDR
                     IMINTYEEMFKNDKDIMDIAKANSYEDLVSTFGKKYFKRGIVKTKNRNDRLVKEILTN
                     NNLQNYMIHYLAEKIYEEANR"
     gene            complement(9183..9353)
                     /locus_tag="PC460_001482"
     CDS             complement(9183..9353)
                     /locus_tag="PC460_001482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600373.1"
                     /translation="MNNDKPFIEFEEEVDFILTECKKEGVSIDRDTIELILDLDLKFL
                     ELKGIATPLEDE"
     gene            9634..9969
                     /locus_tag="PC460_001483"
     CDS             9634..9969
                     /locus_tag="PC460_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600374.1"
                     /translation="MIKLWKKEDLNLLLEYPKEVVENVDNVINILDENYGCNRKLTDD
                     GGYVCIIEDIKEVENLKSNILKGLVEEFSDVIYEDEVNTYNSTLYLLSSDYSVTVISK
                     NEETEYLLK"
     gene            10033..10491
                     /locus_tag="PC460_001484"
                     /pseudo
     CDS             10033..10491
                     /locus_tag="PC460_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            10631..10924
                     /locus_tag="PC460_001485"
     CDS             10631..10924
                     /locus_tag="PC460_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600375.1"
                     /translation="MFKKENRYMTKEIAENLPIEIAILLWNLIDDLTTEKDYLQLFEI
                     NPIGLGVVEIVHKQEVPEYEARIYIQNDKIKDKLKIYVIDNVEYRTMMFSNEY"
     gene            10951..11547
                     /locus_tag="PC460_001486"
     CDS             10951..11547
                     /locus_tag="PC460_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600376.1"
                     /translation="MINNKSEIINKLFFNELKELIDKYNNIDDKTVDVIEKIFTCIED
                     EEIKNYLIRDNCKLQELVNTYNKLEDLNIDEAIFFAWYNLTINTISIDKASEYYDDLT
                     TSNYIEVENHIIYTNERDLRKYAEDELETMLSMEHHIDRLLHKDMLIDMWLNNTSKEE
                     VIEEILMSDDIEDILNLSPEYAFTLANGIEYRYSEKEE"
     gene            11700..11969
                     /locus_tag="PC460_001487"
     CDS             11700..11969
                     /locus_tag="PC460_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HDF2600377.1"
                     /translation="MTNNNNNCDYKGIYADMVEVLGEEIVIKLHKYYRGQQITFPMKL
                     YSIEYVERYIVKNYRTKTIKEMCRELGYTEGWIKQLINKNRLNSK"
     gene            11995..12237
                     /locus_tag="PC460_001488"
     CDS             11995..12237
                     /locus_tag="PC460_001488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600378.1"
                     /translation="MILTVLAILMGTGIFFLINSMLDITYFGCGPIGFLWFGCFIVSY
                     AILTSLGGIVLGLLKWIVIGLIILFVIGLIANKIND"
     gene            12443..13576
                     /locus_tag="PC460_001489"
     CDS             12443..13576
                     /locus_tag="PC460_001489"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012938.1,HMM:NF016983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2600379.1"
                     /translation="MQDFDFISETIKKFQSDLENMDKVNILVVGKTGVGKSTLINNIF
                     REELAETGIGRPVTQHLKKISKEGLPINLYDTKGLELEEDVQETIKKEILDEIENCNR
                     NSLERNDKSELMHVCWYCINTSVARCEETDIKWINELSEKIPVIVVLTQGFPRKKARE
                     LKSSIDDLNLNCKNVIPVLAEQLVEEEDEIVIKSYGLDKLVEVTYQVIPKEVKKAFVN
                     VQKVDIKKKVEESRKWVLGYVGGSGAIGFSPIPFSDAPLLATAQVAMISHITVIFGLQ
                     VDKALLTAIVSSIGGVTSATLLGKTLVSNILKFIPGAGTIAGGAISGSVAALTTTALG
                     MAYISVLEYILNENMKGNEVNNTEIAEKMKDAYKDQLGMLSKK"
     gene            14340..14585
                     /locus_tag="PC460_001490"
     CDS             14340..14585
                     /locus_tag="PC460_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600380.1"
                     /translation="MRSVTVEDGDERFAIEKIYIKSLQRYEVRICLYRDCRDRINKLI
                     PRPVDLTMDKFVALILVGLKAGVLDDDFKQELIKGLA"
     gene            14933..16294
                     /locus_tag="PC460_001491"
     CDS             14933..16294
                     /locus_tag="PC460_001491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600381.1"
                     /translation="MKGNINKKDIDLSKITFDKFLEDEGLQNRERDYLYVDDYDNTLD
                     TQEFKLHIIEENTDNTYIDKDKYWYLDLDDSLFEYEEDEDTIAKTLYSLIDIRIKFLK
                     HVEYRKYINLGVIDEEYFDEIKSFLKFYIVAKTKKNELGNSNLKQMAIDIFDKERFED
                     DISDDDMENISEWIGEDQNFKMFYDKVCRDLSNSTQDKEILKLAGIRNNKVKHYKGRK
                     ISRKCKLELKSLTKMWEYETKLTHDANKAHKKIKSNMLEKMTDKNYKNIYEYLNNEII
                     NSNEEYKNIYFYSLELKLANELRKCTLKNLNKIKDIENQVKFISYTIAYIHIVPVLTI
                     REKLSDKLFNILSTNEGKDIEKFTKEVSLIMRIINIECLELLNIVSKNNYYKNFSEKE
                     LEEFKSIYNINNGRKSFENKYKIKVDYNIDDVRKYGEVIKAINIESKKKIVDRIKKKR
                     KSN"
     gene            16484..16801
                     /locus_tag="PC460_001492"
     CDS             16484..16801
                     /locus_tag="PC460_001492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600382.1"
                     /translation="MTNFKSVKVELILLIECEEGNRNELEGMIDEGVLNEKEYSLTIL
                     GSTEYEDNARAIYILMNTGGSYEKNLQRLSRLHLKIENLLKDTNVKYRGISLVPNIVK
                     WDK"
     gene            16813..18576
                     /locus_tag="PC460_001493"
     CDS             16813..18576
                     /locus_tag="PC460_001493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600383.1"
                     /translation="MSKAINREELIKLYEEIGLNVLDNNTVMNSSNKKICNFLVNIIE
                     QRVIDGKMYLCINAMGINGELLSEVILDSNTINRTTWVIENWGFKYSIDKKYINDFIE
                     LIQHLCMNIKTKYIYDHTGWIYQDNKWIYLHGGGVIGGSDVQVELDESIDNYILPDKI
                     SDINKACEMSLKLVDLIEDDKGIIFSSLVYLSPLIEIISRKIKPPENIVWTYGKTGSR
                     KTAVSKVVLSHFGDFNKKIPATFNDTITAIELKANKLKDSLYLCDDFAPKQDFRDKKI
                     QDSKAESIIRMYGDRVGKGRSSNKLDIKDAKRPRGMILITGEDVICGESSNARLLSIE
                     LNRSSVDLNILTELQNNSHLLGEAMRGYIEWLLSEMGNIDELSNILLYNFKSYRDEIR
                     EQYKDDIHGRTIESSAWILVGFSCMLNYIRDKGVIDKYEDDEYMDRAIKVVERLIEEQ
                     IKLISRNSPIDIFLNTLKESINSKSIRIATLNDQNQVIEDKLEYICGYKDKEYYYFYT
                     DKVYSFIEFENKKRGIHLGISQRQLVKQLRDSGIVKVDSNNDSPKKTIHTKDASDSTG
                     YTTIRPRMLQIERSIIENFQI"
     gene            18639..18794
                     /locus_tag="PC460_001494"
     CDS             18639..18794
                     /locus_tag="PC460_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600384.1"
                     /translation="MTETYKISIGRKDLYSKDYKVVERTDYEKRHTVNISRSLVDILS
                     ILVKEDR"
     gene            18881..20446
                     /locus_tag="PC460_001495"
     CDS             18881..20446
                     /locus_tag="PC460_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2600385.1"
                     /translation="MTGKKAVVYARFSSDNQRDESIDAQLRAINEYADKNNIKIVNQF
                     IDRAKSATSDKRPEFQNMIKFCEADTTGISMVIVHKLDRFSRDKYDSAMYKQKLKVKG
                     IRVVSVLENLDNSPESLILESVIEGMAQYYSANLAREVAKGQKENALKALHNGGDAPL
                     GYDVAHDKTYTVNEEEAQAVKIIFDRYIKGYSYSKIIDELNILGYKTKRGNKFGKNSL
                     HGILNNEKYTGVYVFNKTQRKGVDGKRNGHKQKSEEEIIKIEGGMPQIIDKGVFKQAQ
                     DMIQKRKKTPGAHKATTTYLLTGLIKCGECGHAFQGNKRKDSYGNEYVSYRCGCRKQK
                     RECNNREIKRDYLEEFILQELEKNILNDEAIPILSKALNEKLNDKNESNAELLKNLEQ
                     KLDKVNKEISNILNAIMNGIVNSMLKDKLDELEQVKFNLDLKMNELKIENKVVDGVGV
                     TEDQIRGMFSRFKEFVLERNIPECKKFIGDYIKEVIVYKDHIEVIFNVVFCFVDNDIT
                     HDLHIITERGKIT"
     gene            20592..21884
                     /locus_tag="PC460_001496"
     CDS             20592..21884
                     /locus_tag="PC460_001496"
                     /inference="COORDINATES: protein motif:HMM:NF014177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDF2600386.1"
                     /translation="MADIFTVLNYQGSKKRLLDFIYENTQEYIDSDKAFLDICSGTSA
                     VAYNMKRHFTVYTNDSENYAYQISNSIVKNNERYNFSQSDEMIIREKYNINLQRLNNI
                     FNDMVNQEDMLLEQEDADGLIELYSNCPCIWKNNVDIEIEGNTIQSIYDLRKCKRDIP
                     NVLFTLYYSSSYFGIKQSMEIDSIRYAIEFIESEITKSILFTSLFFAMKECVFAKDGH
                     MAQPLNMETGKKRMLRLKARSIYDIFIDKLKEFNTEEFVLSQKENKCFNKDFRTLLET
                     EESIKDEVGFIYIDPPYTDMQYSRYYHILNTVVLYDYPEISLSRGTISKGIYREGRYQ
                     SPLSQRKHALNEIDFLFSFCKENKINVALSYAYPIDTEKQATNRYTASIDDLINVAKK
                     HFGDNVKVVHEEYSHANNRNSDKKNVLEYLIMGIHNKE"
     gene            21887..23506
                     /locus_tag="PC460_001497"
     CDS             21887..23506
                     /locus_tag="PC460_001497"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDF2600387.1"
                     /translation="MDKINKLKERIANIEATNKNDIYSSNLYWSQKPFNICDEIIEHL
                     SEDGDIIFDPFMGSGVTVIEALKKEYKRNAIGCDINDAPIFIVKTLLEKYNVPKIEKD
                     IKELNNKLSKLSTYYSTKCRECGEVVTISKTIFDKENRNAKCELKSIHYKCKECKNDF
                     KGEPNETEISKFFIDEELKNIDDFQFIANSRLAVGEGDTVRTIFSNRNLIVLDKILGH
                     IDEYKNSESYEPMKYILLSILHLSKITDTHSNSQWPLWIPKKDCVEKNVVELFIKNSK
                     KTIKALKLASKYLCENRENVELIKNLDRGKFKLIKDGVQNIEDDVIPNEFVDLIITDP
                     PYMGQVLYSEYMQLYKPFMDLEFELENEIVISSAPARDKGKVLYFDMMNSAFENISRV
                     LKVGKYMCMYFHDSNLDVWDNIIDIMTNNNLKYMGQIHVSKNKNTLKNILSPKKSLNG
                     DCIIFFKKDLNINYDINEVDNIEDKINNIAQAIIDIKGYASTPELYDNGALEYIISNG
                     KLKEISKKYKDLTSIFEQRFNWDKERGVWTV"
     gene            complement(23631..24782)
                     /locus_tag="PC460_001498"
     CDS             complement(23631..24782)
                     /locus_tag="PC460_001498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600388.1"
                     /translation="MKKLTIRLKDDFHKDLLDLVAFKNDSINSYVINLIKNDLSKNTN
                     LLRSYDNMIRDNITNVLITQKINELKAIQDGIFKEYKVNDILSNSKIYEVMTANSLNH
                     ILIPGHSGSRDAKDEFGNEFEYKHFKKSSSNHSWTFNDFSDTSIAKLKKAKAVIFAHI
                     NDVDFPYPGKLDWYYSIPGDIMCEYLLKYTQSIQNSRKMINVSPNQLESRLNASKIIP
                     SAQNGVYDKYLSAIYKASSELEELTGVKNLLTSNKFWELIVALELNHQVNPEQGGREG
                     AHDAYDSEGNPYEYKVSKNHAWNFQDISDNVLNKYYNDKAIILAVVDKENIEVEHIYS
                     TEPADVVPLLRKKLQEKINRCLEKGSPIRRLQVSLSKSDLTRVNAKKLY"
     gene            25288..25503
                     /locus_tag="PC460_001499"
     CDS             25288..25503
                     /locus_tag="PC460_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HDF2600389.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            25503..25982
                     /locus_tag="PC460_001500"
     CDS             25503..25982
                     /locus_tag="PC460_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HDF2600390.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFDSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            25988..26218
                     /locus_tag="PC460_001501"
     CDS             25988..26218
                     /locus_tag="PC460_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600391.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(26334..27500)
                     /locus_tag="PC460_001502"
     CDS             complement(26334..27500)
                     /locus_tag="PC460_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2600392.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPLRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKYLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQGPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYINNNYVFCNELGIPIDDKRPLRNLKSILNSLNIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(27490..28242)
                     /locus_tag="PC460_001503"
     CDS             complement(27490..28242)
                     /locus_tag="PC460_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2600393.1"
                     /translation="MNKSYIPKYTHKFPTNIPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILINNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(28334..28825)
                     /locus_tag="PC460_001504"
     CDS             complement(28334..28825)
                     /locus_tag="PC460_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2600394.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(28822..29020)
                     /locus_tag="PC460_001505"
                     /pseudo
     CDS             complement(28822..29020)
                     /locus_tag="PC460_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29077..29838)
                     /locus_tag="PC460_001506"
     CDS             complement(29077..29838)
                     /locus_tag="PC460_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600395.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDIK
                     EIIKNDVLTKQSIDELLDIGLSEESIETIYDYFSDSTDNGSNIRLKKLISQKENITIK
                     NNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            29985..30185
                     /locus_tag="PC460_001507"
     CDS             29985..30185
                     /locus_tag="PC460_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600396.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            30189..30503
                     /locus_tag="PC460_001508"
     CDS             30189..30503
                     /locus_tag="PC460_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600397.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            30525..31268
                     /locus_tag="PC460_001509"
     CDS             30525..31268
                     /locus_tag="PC460_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600398.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKKIKIKFGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(31974..33887)
                     /locus_tag="PC460_001510"
     CDS             complement(31974..33887)
                     /locus_tag="PC460_001510"
                     /inference="COORDINATES: protein motif:HMM:NF020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HDF2600399.1"
                     /translation="MSIKLDDKTHNLRINQATSVFGPGAMLDFIDQTLMTAHPNYWTN
                     TIKIQDDRLQATLRVNELRTPPTIDDRSSVPFVRFPRWYFCPKCRKFMPIEDWEKLYK
                     NAHKDKPMKIPICLTCSTKKSTKQLVPTGIITICEHGHIDDFPWVKWVHFRNKSGRKE
                     ICENPQILMKTGKSNLGLEGIELECSCGAKANMKGAFMRLKSSKSNDYGVPSEYKEMF
                     KCSGNSPWYNKKVNKCTKYSFASQRGASNVYYPKIESSIIIPPYSDTLNSKVLSSKGY
                     DTFKKFLDRAIKKNKLERFMDEDFEDFASDIAKETGFDTDKVSIVLKDLLPFEDSSPS
                     TTTRNQYREEEYEALIGNIDAKSKKTNDFTIEEQNLSDYDIPNFSKIVLVKRLREVRA
                     LTGFSRVNPPDNNIFGEEDIDDSCNDTKVISLRSRDCKKSFYPASEVRGEGIFIEINN
                     DMVKSWISSNPKIQDNASILNSRYNKDINVNDKAYRKITPKFLLLHTLAHLIIRELSF
                     VCGYATSSLRERIYCNNSENEKVMSGILVYTASGDSEGTLGGLVRQGMHDSLPSILNS
                     ALNRALWCSNDPICIDSTGQGRNSLNLGACHSCTLLPETSCEEYNLLLDRSMLIGDMD
                     NRDIGFFKNLILI"
     gene            complement(33889..37416)
                     /locus_tag="PC460_001511"
     CDS             complement(33889..37416)
                     /locus_tag="PC460_001511"
                     /inference="COORDINATES: protein motif:HMM:NF012493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDF2600400.1"
                     /translation="MNCSELRDKTLDLVRKELLGPGSEDLCENIKYEIITDSPIERYS
                     TGILYPLNNNIDVSNTSEYDGEDNVFTSDDTQNELDRAISMSNETLPSSMGMTFFLKK
                     DAEVINFRVNYATYRNALYTDCHVKYTGDDFYSSNTINEYVELRGSFLYLKQKLTSEK
                     VYNLLKKHDLLDNAEAKFVLYKLANQCPDDENKTGGYVRVPHENLIVSIDMDKNYIKK
                     PISKDLDIVLSKRIYSNDFVSITCMLVNSQSGPKKYNNTIFQPELMVLAEDNNDLEFI
                     DIFTAGSVNTFKKLDSEEISLDLLYRNKKTFATGHGVAIDQDIQNDALIKLCTEFIPH
                     FEVPKLDFNVDELSDVSRELLSMKNLSDISDLSKDKKIYFLYKFVDAYDIWIDDLEED
                     SKDLSDVFKDVAYEHIKNCRDTKSRMLEGINLIKSNDEVYRAFSLMNRAILMQRCHSI
                     HITDRLPGSNNLDYIDYSNDNSAWRPFQLAYILTCLSSIENPDSKHRDLVDLLWIPTG
                     GGKTEAYLGLSAFTIFLRRIRNKENGNGTAIIMRYTLRLLAAQQFIRASILICACEII
                     RKENKSELGKKEISIGLWIGSDQTPNFNDEAEEMLNNLTGNNIKFRSSINSEYELDNL
                     KSKYNKFQLLKCPWCGTKLVKEWDKTSKKAVGDWGYRIKSRPKKFYLCCPEEDCSFED
                     MLPIQVVDEEIYNNPPTLLFGTVDKFAMITWTTEVASLFALDDDNSNLSPELIIQDEL
                     HLISGPLGTMVGLYETALDAMCSYKGIKPKIIASTATIRKAQNQVKNLYNRNVAQFPP
                     PGLTSSNSFFVKEMPLSKKRGRLYVGVIPSGKTQVTVEVRLMSALLQRLKMMENVELN
                     LLDKYWTLVSYFNSIRELGKCSTLLNDDIKDNMERLCKRVSAHPYQSLRNLNEQRELT
                     SRLTSTKINTTLDELSNTLTSNNLTVDTLLASNMISVGVDVDRLNLMVVVGQPKLTSE
                     YIQASSRVGRSNEGLIFTLYNPTKTRDRSHYEQFFSYHQSFYKFVEPTSITPFSEPAR
                     ERGMHAVLISMIRHILGLQANDLASRFSKNMDGVEDLKNFIIDRSIDIFRENNQNADD
                     NIVNEFKSEISSELDIIMDLWHDKAQISDKDNLLHYYKRPSKFDNKIYNNLLIPYDKD
                     VITNEKQTLNSMRNVDKQCTVEIIELEEN"
     gene            complement(37434..>40014)
                     /locus_tag="PC460_001512"
     CDS             complement(37434..>40014)
                     /locus_tag="PC460_001512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600401.1"
                     /translation="ICEIYFNKQRLTNSKYYQDNINVLNYIINEKNNNLIINYNDKYI
                     YAIYLLNIAINSYLNNTLNPNNHILTKLDKGMPLLYKGQIVIFKNINNTHITLGHKNK
                     TDTTLIPIDSAYMLITYNGHLEVNSMGKTPASKTFQTKNVLSSLGFSDLKNMTGVIND
                     STLIILPCKDDISDLVSNIKIKDINNTYKFTELFPCSYISSTGAETDYPGNHAKQIPL
                     LKFTTNISSAFEIIKSDKSIKNVFIIGDYPIYKHINDFERILNRKRIESVNIVTSNSN
                     ISNILDLPNIDNLNIYSWSKDVLLTYSQDFENTSTFQKLWLDKLINKNICTTSVDSNI
                     SDLIHQARKSLYYISKYDFDISIKNSLMMCSYSLLKILENTPFNLDFLEETIKLLNLN
                     IATPSAKLENIKYLISSTSPDLELSALFDDVIVKFERIINELSDNNTKFNTLNKLVSS
                     YKYKTFTPNTPVILLQKQYEAIILKNLLKSRNINLQVISAEKFKDSKTIKNLIMFGFY
                     DLKYLDILNSDLIINITLYLYNSELSKYNYYMNQINSARNKIEKNNKIYNLLDIKPFK
                     PTYETNKLEAYSTDSPISDDDSMDEYISNYKFDINLNEIILSLSINQGIYNSKKEHVT
                     SFMISDNNEYIFCTKKAIAYSIDWDKKSVRKKKLSEAKVNDWFLFVDDYLNEEGELVI
                     NVIQKLLELNLLPSHYLDDYKLTNSWKSELKNYISYSKYNYNDVSNSLAVLGEKINPI
                     TIKNWVTNKRLIGPIYLKTFEKIFKVIDSKLDHTEVYDATKSIRSLHTKIKKSIDTFV
                     SDNFFSSKSDIIKDEVSAVVLSMLGNTNDYVTPVQIREIHSCDKYLSPHLVNKVLDLY
                     SVY"
BASE COUNT        15883 a         4428 c         6285 g        13418 t
ORIGIN      
        1 tataatatgt aatatttttg ttttgaaatt tataaattag tattactggt tagagttagt
       61 ttagtattaa gtatattcga agtagttttt tagctatttt catacattta tattacttaa
      121 ttatatatag cctaactaac ttaatatatc agtgaaatat tgactaaaat gcctaaatat
      181 gttatcatac aagttagtca aataatttgg caacattaaa tatttagtaa atttctaaca
      241 tttatggaac aaatttctag caaagaaggt gagatttata tggctggtaa aaaaatatta
      301 gcaacaaaca aaagagcaag acatgaatat tttatagaag aaacttatga atgtggaatt
      361 gaattaaaag gaacagaagt aaaatcaata agacaaggca aagttaacct aagtgatgga
      421 tttgcatctg tagataatag cgaagtattt ttaaaacaag ttcatataag tccatatgag
      481 caaggaaata tattcaatgt tgattcttta agagttagaa aactattact tcataagcat
      541 gaaataagaa agcttattgg agcaactact ataaaggggt attcacttat accacttagt
      601 ttgtatctaa aaaacggtaa agttaaagta gagattgcct tagcaaaagg taaaaaacta
      661 tatgataaac gtcaagatat ggctaagaaa gatgcacaaa gacgtataga aagagaaatg
      721 acaggtaagt attaaactag ataaaactgt attttttaga ataaaattct ttattatcta
      781 gaacataaca ttgattttta ttcttaaaag tattatatta tatatacatt aaaaatttaa
      841 taaaaatggt gataaagttt gagaaaagtc tactggacta taaactactc aagggggcgt
      901 aaaggtttcg acgtgggttt ggaacttgag gctgcatgtc gtgttactct gggtcacgta
      961 aaaactgggg aactttaaaa taaacgcaga cgataatttc gcgatagcag cctagttgct
     1021 gctcgtccct cttagtcctc ctgccgacta agactggacg tcattatgca gggaactact
     1081 ttaggggtgt ctcgacttga agtagattaa ttgggactgg ctaagcatag agcctgccac
     1141 ttggcgctat gtgaggtgag atattagtga agttggctaa gcatgtagat gcagcaagga
     1201 aaagatttgc ggacaggggt tcgactcccc tcgtctccac cattgaaatc cacgaaaaca
     1261 tattgatagt ttagtcaata tgtttttttt gtgccttgaa atgatataat tataatataa
     1321 actaatgtaa aatttaacta aaaggagata gtataagtga gtatagataa taattttaac
     1381 catgttgcat ttctgtggaa tatagcagaa agtttaagag gtacatataa agaagaagac
     1441 tatagaaaag taatgttacc actaatagta ataagaagat ttgattgtct attagatgat
     1501 tatgatagag aaataataaa atcagtatac gaagaatacg acttcttacc agaagaagaa
     1561 agagatgagc tagtaatagt agacttaaaa gaaaatcacg acataaactt acaattttac
     1621 aatgtaagtg attttacatg gaaaaaacta ttagatgata gtgaaaatat aaaatcaaac
     1681 tttgaagaat atctaaacgg attctcaaac aatgtaaaag aaataatagg taaatttaaa
     1741 tttaaagatg aaataaatca attagataaa aaagacaagc tatatgcagt tcttagcaaa
     1801 atgtatgaag tagatctaca tataaactca gtatctaaca ataaaatggg atacatatac
     1861 gaagaaatgt taagaagatt tacagaaaac tcagcagcag gagagcaata cacaccaaga
     1921 gaagttataa gactttgtat ggaaatacta tttatgggta aagaaaactt ccttacagaa
     1981 gaaggaaaag taatatctat agcagacttt tgttgtggta ctggaggaat gctttcaata
     2041 gctgaagact atgtagaaaa aataaatcca agtgcaatag taaatgtata tggacaagaa
     2101 ctacttgatg aatcattcgc aatatgtcag gcagatatgc taatgaaagg gcaaaatcca
     2161 gataacataa gacttggaaa tactttaaca caagatagat ttaacggaga aaaaataaga
     2221 tttttaatat caaatccgcc ttttggtgta acttggaaag acgaagaaaa gaaagtaaaa
     2281 gaagaagcag acttaggttt tgatggaaga tttggagcag gtactccaag agtaagtgat
     2341 ggttcattac ttttcctaca aaatatgata agtaaaatgt atgatgatga agaaggaagt
     2401 agaatagcta taatatttaa cggaagtcca ttatttacag gagatgcagg aagtggagaa
     2461 tcaaacatta gaaaatggat aatagaaaat gatttactag aaggtataat agcactacct
     2521 actgatatgt tctacaatac aggaatagct acatatattt gggtacttac taacaaaaaa
     2581 gaagctaaaa gaaaaggtaa gatacaactt gtaaatgcta gtgaatatta tcaattaatg
     2641 agaaaaagct taggaaacaa aagaaaagaa ataagtcttg aacaaataga acaaataaaa
     2701 gaaatatatg aaaaatttga agaaagtgaa aatagtaaaa tatttgataa cgaagatttt
     2761 ggatatagaa aagtaacaat agaaagacct ttaaaactaa gctttaaagt aaacgaagaa
     2821 gctatagaaa atgtaaaaaa tactactcaa tttataaatc ttgcagtttc taagaaaaaa
     2881 gatatagaag ttaaagaaaa agaagaagct gaaggaaaaa taaaacaaga taagttaata
     2941 aaacttttag aaagctttga caatacttta gagtatatgg acagggataa atttataaaa
     3001 gatttaaaag ctaaatcaaa agagtttgat atacaacttt cagcaggact tataaaggct
     3061 atagttaata ctattggtgt aagaaatgaa gatgcagaag tttgtaaaga tagcaaaggt
     3121 aatatagaaa gtgatagttc attaaaagat actgaaagta taggtcttaa agatgatgta
     3181 tatgagtatt ttgaaaagga agtaaagcct catgttaatg atgcttatat agatgaaagt
     3241 agtataaata atataggata tgagatacca tttactagac atttttataa gtatgaagag
     3301 ttaagaagtt ttgctgagat tatgaaggaa gtagaaagtt tagaaagtga gatagcacta
     3361 aagataagga aggtgctagg gtaatgggat acagatatag aagtgatgat gagttaaagg
     3421 atagtggagt tgagtggtta gggaagattc ctaaggattg ggaagtcaga caattaaaaa
     3481 gggtactatc agatagaaaa gaaaataatg aacctataaa aacaagtaat atactgtcat
     3541 tgacaataga aaagggagtt attccatatt ctgaaaaagg tactggtggt aataaagcta
     3601 aagaagattt aacacaatac aaattagtat atccaaatga tattgtatta aatagtatga
     3661 atgtaattgt tggtgcagta ggactatcaa aatattttgg gtgtgcgagt cctgcatatt
     3721 atatgttata taaaataaat gctaacgatg atataagatt ttataattat atttttaaat
     3781 ctaaattatt ccaggatagt ttaaaaggat taggtaatgg aataatgatg aaacaatcag
     3841 aaacttcagg taagttaaat acgatcagaa tgagaattcc aatggaaaaa ctaagttcag
     3901 taaagatacc aattgctttt aacttagatc aagaaaaaat agcaaacttt cttgatgaaa
     3961 agacttcaca gtttgattca attatatcta agaaagaaaa gttaatagaa agattagaag
     4021 aagcaaagaa aagccttata agtgaggttg ttactggtaa ggttaaggtt gttaagactg
     4081 atgatgggta tgagcttata aagagaagta gtgaagagat gaaggatagt ggagttgagt
     4141 ggttaggtga gatacctaag gaatgggata taaaaagact tagattttta ggaaaactac
     4201 aaaacggtat aagtaaaagt agtgattcat tcggttatgg ttatccattt gttagttata
     4261 gtaatgtata tagaaatata gaattaccac atatagtaga aggtctagta aattcaactg
     4321 aagaagaacg taaaatttat tcagtagaag aaggagatgt attctttaca agaacttcag
     4381 aaacagtaga agaaattggt tttgcttcta cgtgtatgaa aactattgat aattctactt
     4441 ttgcaggatt tttaataagg tttagaccga atgataaaag actatacaaa ggattttcta
     4501 aatattactt tagatgtgat ttaaatagaa aatattttgt aaaagagatg aatttagtaa
     4561 ctagagcatc tttatctcaa aatcttttaa ataatctttc tgttgtattg cctaagtatg
     4621 aagaacaagt tgagatatat aatgcacttg aattaaaagt tagctacatt aatttatcga
     4681 ttaataagtt aaagtaccaa atagaaaaac taaaagaagc taaacaaagt ttaataagtg
     4741 aagcagtaac aggaaaaata gagatactag attaacttct agtatcttaa tatagatata
     4801 gattatacat acaaagttaa ataaaaggga gaattaatgg ataaaaatat atttcaaatt
     4861 aatataaata cagatgtact gataagatta aggaatcaaa taaatgaaga atattatata
     4921 tcttatagaa aagaatttta tgataaaaat aacaaaaaac atcgcgcttg ggataaaata
     4981 tgtgcaataa tggataggct tgacgatact gtagattatt taaataaact caaattaaat
     5041 acaggtaaat atagacgaag tgcatttgac ttctttgaat ttatgaatca ggcttccgta
     5101 gtaatggatt gtataacaca tcttgcaaaa atatttgaag taccatatga agaagttgaa
     5161 acttcaactg aaatttttaa tgagcttgga aaagagggta aaggtacaga taaaaaatac
     5221 tttgaatatc tacgttcatt gtgttcagtc catccaatgg aaactagtag acacaaaata
     5281 taccaagaaa atagttttga atgtagtcct tatgtagttt ggaatgataa aagtatatgg
     5341 actaatgatg ctagcgattt atatgcagtg gtatacacaa gtaaagataa tgatagtttc
     5401 aaaagaattc atatatatat gaatcaaata tttgagtatg taaaaagaag agtaaatttt
     5461 attgaaaata taatagaaca tatagaaaag tatcaacaag aaataattga aatttttagg
     5521 aatactccaa taaagaaaga agaagatttt gaaagttata tagattattt aagaaatcta
     5581 aaaaaagaga tagaaataag atatggtaat gattcttttc atgaattaaa ctaccttata
     5641 gaattatttg atttgccatt atcaaatcct gataatgaag agaagatgaa cttatataaa
     5701 aatgctttaa aatatgctat caaatttgag cataatagta ttcaaaatat gacaaatgaa
     5761 ggttataaga ataatggaat attatatcca caagtgaata taacaactac attactagaa
     5821 gagctaattt cattaaatag tggtagtgag gagagacgta agtacagata tgcaatagaa
     5881 aaaacatcat atctaagcta tgatagttgt tggagtgata gagaatgggc atatgatcaa
     5941 atagatgata tgatgccttt tttagaaaga tacgtaacat ttgataaagc tcaaagtgat
     6001 tttgagcatt atgtattggt aaaactagca ctttatttag agtgcttaga aaatgaatgt
     6061 ttaataaata agaacgttcc taatgataaa aagtttcgta ttaaataatt atagctaata
     6121 aaatagggag agggaggata ttatgttacc aaaagaaaaa cacctagaag aatctattga
     6181 aaatcattta gtttcaattg gatatgaaca aggtcattca caagaatttg accttgaaaa
     6241 ttgcttattt gtagatgatt tatttagatt cttagaaaat actcaaaaag aattactaga
     6301 tgagtttaaa tacaacaggg gaagtaatta caaaaaagct tttatagatt tattaaattc
     6361 aaacatcaaa agaagaggtc ttataagtgt acttagaaat ggtgtagaag atataatgat
     6421 gaacggaaaa tttcaattat tctacaacaa accaaatcta actactaata aaacagcgta
     6481 tgaaaattat aaacaaaata tattcaaaat aacaagacaa ttaaaatata gctcaaagca
     6541 caacaatact ttagatatag tattatcttt aaatggattt cccgttatag taatggaact
     6601 taaaaatcag tttactaatc aaaatgtgta taatgctata aagcaattca aaaaagatag
     6661 aaacccaaaa gaaactttat ttggatttaa tcaaagagtg ttagtatatt ttgcagtaga
     6721 tactgatgaa gtttatatga caacagagct aaaaggagat aaaacattct tcttaccgtt
     6781 taataaaggt aacaatagag gtaagggaaa tcctgtagta gaaggaaaag aaaaaacttc
     6841 ttatctttgg gaagaaatac ttactaaaga tagtttactt gatattataa aaagatttta
     6901 ctttattcaa gaagatgata aaggtaatca aagagcaata tttccaagat ttcatcaatt
     6961 agatgcagta aggaagatag tagaagattt aaagataaat aaagtaggta aaaattatct
     7021 tatccaacat agtgctggta gtggtaagac taacactata gcatggactt cacataggtt
     7081 atcaagtctt catgatgaag aaaataatgc tatatttgac tctataattg tagttacaga
     7141 cagaaaggta cttgataaac aattacaaaa tgctatatat caattagagc acaaaaacgg
     7201 acttgttgtt aagatagatg aagataaaaa ttcatcagac ttagcagatg ctattgtaaa
     7261 tagagcaaag attattataa ctactattca aaaattccag tatgcactac ctaagataga
     7321 acaacttgat aaaaggcgtt atgcagtaat tatagatgaa gcacattctt caacatctgg
     7381 tgaaaatatg aatgcattaa aagaaacttt agcaggaaag actttagaag aagctaaggt
     7441 aattgatgcg gaaaatgaaa agaatgaaaa gtctagtata gataagatga atgagcttat
     7501 agagaaaaga acaaatttag gaaatataag tttctttgct tttacagcta ctcctaagaa
     7561 taagactatg caagtatttg ggaggatagg tgaagatgga ctacctcatg aatttcattt
     7621 atattctatg aaacaagcga tagaggaagg atttatatta gatgtactaa aaaactttat
     7681 gcctataagt gtttattaca aagtaggaaa gaaaattatt gataaccctg agtttgataa
     7741 gggagaggct aagagggcta taaataagtt tgttagttta aatgagcaca atataaggca
     7801 aaaggttgaa actatagtag atgattttgt agataatagg tctatgtggt tagaaggtaa
     7861 agctaaaggt atgatagtta ctgcaagtag attacatgct gttagatata aactagctat
     7921 agaccaatat ataaaagaaa aaggatatga tatgaaagca cttgttgctt tttctggtac
     7981 tgtaaaagat ggtgatgatg agtatacaga ggtaggtatg aataaggaag tagcaccaga
     8041 tattactgat tcaaatttac ctaatatatt tgataaaaat gaatttaagc tattaatagt
     8101 tgcagaaaag tatcaaactg gatttgacca accaaaactt tgtgctatgt atgtggataa
     8161 aaaactagat ggtgttaaaa ctgttcagac tttatcaagg cttaatagaa cttatcctaa
     8221 taaacaaaca tttatattag atttccaaaa tagtgtggaa gacatacaag atgcatataa
     8281 gccatatttt gaaatgacta atattgataa ggtaacagat ccaaatgtag taaatgacct
     8341 ttggtatcag ttacatgaat atgcagtata tactgatgag gaagtaaatg attttacagt
     8401 attattctat aaagagaaaa gaacttcttc tcaagatgct aagatgaata atttaattga
     8461 taaggcagtt gaaaggtatt gttatttaga agaagatgat gaaaaaaaat ctgatgagtt
     8521 taagaaaaaa tgtcaaaagt atattacttt ttataatttt ttaattcaga tttatcctat
     8581 aaagaactta aatcttttaa gattgcaagt ttatttagta gcacttttaa agaagttacc
     8641 taaaaagagt aaacaaagaa tagaccttga tgatatgtta agccttgatt attataaatt
     8701 gcaaaagctt ggattagatg ataaaaaagg aacggatata tccttatata atggagaagg
     8761 tgagttagtt ggtatatctg aaactgcgac ttctcgtgta agtgaagctg atgaagagta
     8821 tttagaggat ataattaaaa aaataaatga tgtttttggt gtaggactta cggaagatga
     8881 tagaattatg ataaatactt atgaagagat gtttaagaat gacaaggata tcatggatat
     8941 agctaaagct aatagttatg aagatttagt tagcactttt ggtaagaagt attttaagag
     9001 aggtatagtt aaaaccaaaa atagaaatga tagattagtt aaggagatat taactaataa
     9061 taatttacaa aattatatga ttcattattt agctgaaaag atatatgaag aggcaaatag
     9121 ataagatacc tatagagagc taattaactc tccataggta ctttattatg agctattgta
     9181 tattattcat cttctaaagg tgtagcaatt ccttttaatt ctaaaaactt taagtctaaa
     9241 tcaagaataa gctctatagt atctctatct atagaaacac cttctttttt acactcagta
     9301 agtataaaat caacttcttc ttcaaactct ataaatggtt tatcgttatt catatttttg
     9361 ctcctttgat taaatataat gtatttactt taaattatat caaagaagag aaaaaaataa
     9421 cattatttgt aaaaaactat atatatataa tatatagttt taccatatat agataaactg
     9481 tttttaatta aattataaat atatattata ttaatgaatc tatattaaat agatagttga
     9541 gtaagtagta ataccaaaaa tattaaaaag agatgttggt agaaatacga acatctcttt
     9601 tttagtgcaa aaaattaaaa ggaggaagtt aatatgatta aattatggaa gaaagaagat
     9661 ttaaatttat tattagaata tccaaaggaa gtagttgaaa atgttgataa cgtcattaat
     9721 attcttgatg aaaactatgg atgtaacaga aaattaacag atgatggtgg atatgtgtgt
     9781 ataattgagg atattaaaga agtagaaaac ttaaaatcaa atatattaaa ggggttagta
     9841 gaagagttta gtgatgtaat atatgaagat gaagttaata catataattc aacactatac
     9901 ttgttatcta gtgattatag tgttactgta atttctaaaa atgaagaaac tgagtactta
     9961 cttaaataag catatacatt gataaggtgt atatgcttat tttaatttta ataaataaag
    10021 gagaaaagca gaatgaataa aaagagaact cctgccaaga gagttgatat agttttactt
    10081 aaattagtaa aagaacgtag tgttctatat gaaactagaa aaataagtaa tctttatgat
    10141 gcttatagat tagcaaagaa ttttttataa atagtgctag agaaaagttt gtagtggtat
    10201 gtttagatac aaagaattgg ccagtagcat agaggttgta agtataggca ctgtaaattt
    10261 atctatggta tatccaagag aaatatttaa agtagctata ttaaccaatt catctgaaat
    10321 gatatgcttt cacaatcacc caatgggtaa tactgacttt agcaaggaag atagttataa
    10381 ctaatatgct acagaagcgt ggagaaatat tatgcattga attagtagat acatatagtt
    10441 attgtagatg aggataaata ctttagcttt aaagaaaact gtaggatata agagtcttag
    10501 cttaaataaa ttaggctaag gctttttcta tgttccaaaa caattaaaaa taaatcaaat
    10561 ttaggttaag cccggaaagc ccttgaaaaa aataatacat ataaatttaa aactaaatag
    10621 gagttgtaac atgtttaaaa aagaaaatag gtatatgaca aaagagatag ctgaaaatct
    10681 accaattgaa atagctatac tactatggaa tttaatagat gacttaacta cagaaaaaga
    10741 ttatctacaa ctatttgaaa taaatcctat aggattagga gtagttgaga tagttcataa
    10801 gcaagaagta cctgaatatg aagctaggat atatatacag aatgataaaa ttaaagataa
    10861 attaaaaata tatgttatag ataatgttga atatagaaca atgatgtttt caaatgaata
    10921 ttagttaatg attaaaggag agagagtaat atgataaata ataaatctga aataattaat
    10981 aagttatttt tcaatgaatt aaaagagtta atagataaat acaacaatat agatgataaa
    11041 actgtagatg taatagaaaa gatatttaca tgtatagaag atgaagaaat aaagaattac
    11101 ctaataagag ataattgtaa gctacaagag ctagtaaata catacaataa acttgaagat
    11161 ttaaacatag atgaagctat attctttgct tggtacaact taactataaa tacaatatct
    11221 atagataaag caagtgagta ctacgatgat ttaactacaa gtaactatat agaagtagaa
    11281 aatcatataa tatatacaaa tgaaagagat ttaaggaagt atgctgaaga tgaattagaa
    11341 actatgttaa gcatggaaca tcatatagat agattactac ataaagacat gttaatagat
    11401 atgtggttaa acaatacttc aaaagaagaa gtaatagaag aaatattaat gagtgatgat
    11461 atagaagata tattgaattt atctccagaa tatgcattta cattagctaa tggaatagaa
    11521 tatagatact cagaaaaaga agaataaaat aagtatatgt actgaaaaat acgggcttta
    11581 agggcttata cattaaagta catatttgaa aataccatag attttacaag agaaaatcag
    11641 aatgaaactt tttcaaatac tagttattat taattagtgc tagaaaagga ggtggagtga
    11701 tgactaacaa caataataat tgtgattata agggcatata tgcagatatg gttgaggttt
    11761 tgggtgaaga aatagtcatt aaattacata agtattatcg tggacaacaa ataacatttc
    11821 caatgaaact atattctatt gaatatgtgg aacgatatat agtgaagaat tatagaacaa
    11881 aaacaataaa agaaatgtgt agagagttag gatatacaga gggatggata aagcaattaa
    11941 ttaataaaaa tagattaaat tcaaagtaat tagagaagga gattgacagt agatatgata
    12001 ttaacagtat tagcaatact tatggggaca ggtatatttt ttctgattaa ttcaatgcta
    12061 gatataacgt attttggatg tggcccaata ggatttttat ggtttggatg ttttattgtg
    12121 tcatatgcaa tattaacatc attaggtggt atagttttag gattgttaaa gtggatagta
    12181 ataggattga ttatactatt tgtaatagga ttaatagcga ataagataaa tgattaaatt
    12241 actgtagatt attttgataa aataattatt taatatattg aggcattaca tgatagaaga
    12301 ataaatataa atgacttaaa atctttaact taactaaaca aaaaatatat tatttattac
    12361 aaaaaatgac aaaaaaatta aatattatat tgtattatga caatataaaa cattggagtt
    12421 tataaattag gaggaatatt ttatgcaaga ttttgatttt ataagtgaga caataaagaa
    12481 atttcaatcc gatttagaaa acatggataa ggtaaatata cttgttgtag gtaaaacagg
    12541 agtaggtaaa agtacattaa taaataatat atttagagaa gaattagcag aaactggaat
    12601 tggtagacct gtaacacaac acttgaaaaa aataagcaaa gaaggattac caataaattt
    12661 atacgataca aagggattag agttagaaga agatgtgcaa gagactataa aaaaagagat
    12721 attagatgaa atagaaaatt gtaatagaaa ttcattagaa agaaatgata aaagtgagtt
    12781 aatgcatgta tgttggtatt gtataaatac atcagtagct agatgtgaag aaactgatat
    12841 aaaatggata aatgagttaa gtgaaaagat accagtaata gttgtattga cacaaggatt
    12901 cccaagaaaa aaagcaagag aattaaaatc tagtattgat gatttaaatt taaactgtaa
    12961 gaatgtaatt ccagttttgg cagagcaact agtagaagaa gaggatgaga tagttataaa
    13021 gtcatatggt ttagataaat tagtagaagt tacatatcaa gtaataccaa aagaggtcaa
    13081 gaaagctttt gttaatgtac aaaaggtaga tataaaaaaa aaggttgaag agtcgagaaa
    13141 atgggtttta ggatatgtag gaggatcagg agcaatagga ttttcaccaa taccattttc
    13201 agatgctcca ttattagcaa ccgcacaagt tgctatgata tctcatataa cagtaatatt
    13261 tgggttacaa gttgataaag ctttactaac ggcaattgta agttctatag gaggggttac
    13321 atcagctact ttactaggaa aaacattggt atcgaatata cttaagttta taccaggagc
    13381 tggaactata gcaggtggag cgataagtgg ttctgtagcg gcattaacaa ctacagcatt
    13441 aggaatggca tatataagtg tattagaata tatactaaat gagaatatga aaggtaatga
    13501 ggttaataat actgaaatag ctgaaaaaat gaaagatgca tacaaagatc aattaggaat
    13561 gttatctaag aaataattaa ttcaagataa agtataagat taattatagg gaaaaagtaa
    13621 aatttaaagg tatatttggt aaaacataaa caaaaatgat aatggcgtca atattatttg
    13681 ttgtcatatg tagtagctta gtatatatat tattgagtga taggtttaat cataagttaa
    13741 tttacttaga gaaagatata ttagatttaa gaaataaaaa tttgtaacta gctaataaag
    13801 gatcagtata gaaacagata aacaagagga taatgtagat attaaatcta aaaacactaa
    13861 taatgaagaa tataaaactt caaaactatt taggcactag atagagtatt tgctataatg
    13921 aataagctaa tagagaaatt aaaacgttta tagtaagata actaattcaa atacatttag
    13981 tggacttcaa ggaacatttg atacactatc aaactatggt agccaattga tagaccaatc
    14041 atctaaattg aatttatcaa atgttggatg gttagaaata tctgatataa ttacaagtat
    14101 aggaaaggac tattagattc tgaaaatatg tcatttgaag cattaaatat aatgggaaaa
    14161 ataggaaaat ttataaatta tattgaaatt aattatatta actaattaaa tattgataca
    14221 taatgccttg ctacagttaa atgtggcaag gcattatttt tgtaaagaaa ggaagaatta
    14281 acatggcgaa gcaaagtaaa gaggaactat ttgaaaaaga aactaaatat tgcaagataa
    14341 taagaagtgt aacagtagaa gatggagatg aaagatttgc aatagagaaa atatatatta
    14401 agtcattaca aagatacgag gttagaatat gcctgtatcg tgactgccga gatagaatta
    14461 ataaacttat tccaaggcct gtagatttaa ctatggataa gtttgtagca ttaatactgg
    14521 taggattaaa ggctggtgta ctagatgatg atttcaagca agaactaatt aaaggtttag
    14581 cttaggaggt agaatatgac aatagaagag tttactagtg gagtaatgtt tgggatttca
    14641 atttttagtg cagtattaaa gcaagtatta gaaaataaaa aatagtatac aggaggtatg
    14701 agtatgattg gatttataat aggaagtata ataggaattg gactatgtat tagtatagag
    14761 atactgatat cgaaaaatgc cataatatac taagttatat ttaaattatt aatagattca
    14821 tatataattt atttaagccc ttaaagccca gataagttat attaaagtaa agtttgtatt
    14881 tatgtaaatt aaataatata tattattata aatttgaaag gactttataa atatgaaagg
    14941 caatataaac aaaaaagata tagatttaag taaaataaca tttgataagt ttttagaaga
    15001 tgaaggatta caaaatagag agagagatta cttatatgta gatgattatg acaatacttt
    15061 agatactcaa gaatttaaat tacatataat tgaagagaat actgataaca catatataga
    15121 taaagataaa tactggtatt tagatttaga tgatagttta tttgaatacg aagaagatga
    15181 agatactatt gctaaaacat tatatagtct aatagatatt agaattaaat ttttgaaaca
    15241 tgttgagtat agaaaatata taaatctagg agtaatagac gaagaatatt ttgatgagat
    15301 taaatcattt ttgaaatttt atatagtagc aaaaacaaag aaaaatgagc taggtaattc
    15361 aaatttaaag cagatggcta tagatatttt tgataaagaa aggtttgaag acgacatatc
    15421 agatgatgat atggaaaata taagtgaatg gataggagaa gatcaaaact ttaaaatgtt
    15481 ttatgataaa gtttgtaggg atctctcaaa ttctacacag gataaagaaa tattaaaatt
    15541 agcaggaatc aggaataata aagtaaaaca ttacaaagga agaaaaatta gtagaaaatg
    15601 taaattagaa cttaaatcat tgactaaaat gtgggagtat gaaactaaac tgactcatga
    15661 tgcaaataag gcacataaga aaataaaatc taatatgctt gaaaaaatga cagataagaa
    15721 ttataaaaat atatatgaat atttaaataa tgagattata aatagtaatg aagaatataa
    15781 aaatatttat ttttatagct tagaattaaa gttagcgaat gaattaagaa agtgtactct
    15841 taaaaactta aataaaatta aagacataga aaatcaagta aaatttattt cttatactat
    15901 tgcttatata cacatagttc cagttttaac gataagagag aaactaagcg ataagttatt
    15961 taatatattg tcaactaatg aaggtaagga tatagaaaaa tttactaaag aagttagtct
    16021 tataatgaga attataaata tagaatgttt ggagttatta aatatagtta gtaagaacaa
    16081 ctattataaa aattttagtg aaaaggaatt agaggagttt aaatctattt ataatataaa
    16141 taatggaaga aagtcatttg aaaataaata taaaataaaa gtagattata atatagatga
    16201 tgtaagaaag tatggagagg ttataaaagc aataaatata gaaagtaaga agaaaattgt
    16261 agatagaatt aagaagaaga gaaaatctaa ttagattttc tctatttttt ttgaaaaaaa
    16321 taaaaaatat ttggtgaaaa cgtttatctt taggtgatat gagtagtcat tatttattat
    16381 ataatcatat ttgtaggaac gagctaatta ataaaatcta agaacaatag cttaatgatg
    16441 aaaaagtacg gcctttcggg ctttgacaac aggaggtata ttaatgacaa actttaaaag
    16501 tgtaaaggta gaattaattc tacttattga atgtgaagaa ggaaatcgta atgaattaga
    16561 aggtatgatt gatgaaggtg tattaaatga aaaagaatat agtttaacta tattaggtag
    16621 tacagagtat gaagataacg caagggctat ttacatactt atgaatacag gaggatcata
    16681 cgaaaaaaac ttacaacgat taagtagatt acacttgaaa attgaaaact tacttaaaga
    16741 tacaaatgtt aaatatagag gtatatcttt agtaccgaat attgttaaat gggataaata
    16801 ggaggaatac aaatgagcaa ggcaataaac agagaagaat taattaaatt atatgaggaa
    16861 ataggattaa atgttttaga taataacacc gttatgaatt catctaataa aaagatatgt
    16921 aattttttag taaatataat agaacaaagg gttatagatg gaaaaatgta tttatgtata
    16981 aatgctatgg ggattaatgg agagctatta tcagaggtaa tattagattc gaataccata
    17041 aatagaacta catgggtgat tgaaaattgg ggttttaaat attctataga taaaaaatat
    17101 attaatgact ttattgaatt gatacaacac ttatgtatga atataaaaac taagtatata
    17161 tatgaccata caggatggat atatcaagac aataagtgga tatatctaca tggaggagga
    17221 gttataggag gttcagatgt tcaagttgag ttagatgaat caatagataa ctatatacta
    17281 ccagataaaa ttagtgacat aaataaagca tgtgaaatga gtttaaagct agtagactta
    17341 atagaagatg ataaaggtat tatattctct tcattagttt acctatctcc acttattgaa
    17401 attatcagta gaaaaattaa accaccggaa aatatagtat ggacatatgg aaaaacagga
    17461 tcaagaaaaa cagcagtatc aaaagttgta ttaagtcatt ttggtgattt taataaaaaa
    17521 attccagcta catttaatga tacgattaca gctattgaat taaaagctaa taagttaaag
    17581 gatagcttat acttatgtga tgactttgca cctaaacaag actttaggga taaaaaaatt
    17641 caagactcaa aggcagaatc cattataaga atgtatggag atagagtagg gaaaggacgc
    17701 tcaagtaata agttagatat aaaggatgct aagagaccta gaggaatgat attaataaca
    17761 ggtgaagatg tgatatgtgg agaatcatct aatgctagat tattatcaat agaattaaat
    17821 agaagttcag tagatttaaa tatactaact gaattacaga ataatagtca tttgcttgga
    17881 gaagcaatga gaggatatat agaatggtta ttatctgaaa tgggaaatat tgatgaatta
    17941 tctaatatat tattatataa ctttaaatct tacagggatg aaattagaga acaatataaa
    18001 gatgatatac atggaagaac tattgagtct agtgcatgga tattagtagg ttttagttgt
    18061 atgttaaatt atattagaga taaaggtgtt atagataagt atgaagatga tgagtacatg
    18121 gatagagcga ttaaagttgt agaaagatta atagaagagc agataaaatt aatatcgaga
    18181 aactcaccta tagatatatt tttaaataca ttaaaggaat caataaattc aaagtcaata
    18241 agaatagcaa cattaaatga ccagaaccaa gttatagaag ataaattaga atatatatgt
    18301 ggatataagg ataaggaata ttattacttt tacacagata aagtatattc ttttatagaa
    18361 tttgaaaata aaaaaagagg tatacatttg ggaataagtc aaagacaact agtgaaacag
    18421 ttaagagata gtggaattgt gaaagttgat tctaacaatg attctcctaa aaaaactatt
    18481 catacaaaag atgctagcga ttcaacagga tatacaacaa taagaccaag aatgcttcag
    18541 atagaaagaa gtataattga gaactttcaa atataactta taatcaagag tgcttatgta
    18601 ctcttgatta tattctatga taaatggagg atataaatat gacagaaact tataaaatca
    18661 gcataggaag aaaagattta tattctaaag actataaggt agttgaaaga actgactatg
    18721 aaaagagaca cacagttaat ataagtagaa gcttagtaga catactttct atacttgtaa
    18781 aagaagatag atagattaaa atatggttac acacttagtg ttagtgtgta gcctttaata
    18841 tagattaaaa ttagtatatt taaactttgg aggaaataat atgacaggaa agaaagcagt
    18901 agtatatgca cgctttagtt cagataatca aagggacgaa tcaatagatg ctcaattaag
    18961 agcaataaat gaatatgcag ataagaataa tataaaaata gttaatcaat ttatagacag
    19021 agcaaaatca gcaacatcag ataaaaggcc agagtttcaa aatatgatta aattctgtga
    19081 agcagataca acaggaatat ctatggtaat agttcataag ctagatagat tttctagaga
    19141 taagtatgat tcagctatgt acaaacagaa attaaaagta aaaggaataa gagttgtaag
    19201 tgtattagag aacttagata attcgcctga atccctaata ttagaatcag ttatagaggg
    19261 aatggcacaa tattacagtg ctaatttagc gagggaagtt gctaaaggtc agaaagaaaa
    19321 tgcccttaaa gcccttcata atggaggcga tgcaccatta ggttatgatg tagcacatga
    19381 taaaacatat acagttaatg aagaagaagc tcaagcagta aagattatat ttgatagata
    19441 tattaaagga tattcatata gtaagataat agatgaacta aatatcttag gatataagac
    19501 taaaagaggt aataaatttg gtaagaatag cttacatgga atattaaata atgagaaata
    19561 tacaggagta tatgtattta ataagactca gagaaaaggt gtagatggaa aaagaaatgg
    19621 acataagcaa aagagtgaag aggaaataat aaaaatagaa ggtggtatgc cacaaataat
    19681 agacaaagga gtatttaaac aagcacaaga tatgattcaa aaaagaaaga aaactccagg
    19741 tgcacataaa gctactacta catatctatt aacaggacta attaaatgtg gtgaatgtgg
    19801 acatgcattt caggggaata agagaaaaga ttcttatgga aatgaatatg tttcatatag
    19861 atgtggatgt agaaagcaaa agagagaatg taataataga gagataaaaa gagactattt
    19921 agaagagttt atattacaag aacttgaaaa gaacatatta aatgatgaag ctataccgat
    19981 tttaagtaaa gcattgaatg agaagctgaa tgataagaat gaaagtaatg cagagctact
    20041 taaaaatcta gagcagaaac tagataaggt aaataaagag ataagtaata tactaaatgc
    20101 tataatgaat ggtatagtaa atagcatgtt aaaagataag cttgatgagc tagaacaagt
    20161 aaagttcaac ttagatttaa aaatgaatga actaaagata gaaaataaag ttgtagatgg
    20221 tgtaggtgta acagaagatc aaataagagg tatgttttca agatttaagg aatttgtact
    20281 agagagaaat atacctgaat gtaagaagtt tataggagac tatataaagg aagtaatcgt
    20341 atataaagac cacattgaag tcatattcaa tgtggtcttt tgttttgttg acaatgatat
    20401 aactcatgat ttacatatta tcacagaaag gggtaaaatt acatagtaaa tatatatttt
    20461 ataaaagtaa atatcagata ttatttagat aaaaattatt tttgaaaata ttagttttaa
    20521 aaaatatatt tatataatta taaaataaaa tggtataata aaactttaaa tatgataaag
    20581 aggtgagact attggcagat atatttacag tactaaatta tcaaggtagt aaaaagagat
    20641 tattagattt tatatatgaa aatacacaag aatatattga ttctgataaa gcttttttag
    20701 atatatgctc aggaacgagt gcagtagctt ataatatgaa aagacacttt acggtttata
    20761 caaatgatag tgaaaattat gcatatcaga tatctaattc aattgttaag aataatgaaa
    20821 gatataactt tagtcaatca gatgaaatga tcataagaga aaaatataat attaatttac
    20881 aaagattaaa taatattttt aacgatatgg taaatcagga agatatgtta ttagaacaag
    20941 aagatgcaga cggtttaata gagctatatt caaattgccc atgtatatgg aaaaataatg
    21001 ttgatattga aatagaaggc aatacaatac agagtatata tgatttaaga aaatgtaaac
    21061 gagacatacc gaatgtgtta ttcacattgt attattcaag tagttacttc ggcataaagc
    21121 agtctatgga gatagattct attagatatg ctattgaatt tatagaaagt gaaataacaa
    21181 agagcattct ttttacaagt ttatttttcg caatgaaaga gtgtgttttt gctaaagatg
    21241 gtcacatggc acagccatta aatatggaaa caggcaagaa aagaatgtta agattaaaag
    21301 caagaagtat atatgacata tttatagata aattaaagga atttaatact gaggagtttg
    21361 tattatcaca aaaggagaat aagtgcttca ataaagactt tagaacatta ttagaaacag
    21421 aagaaagtat aaaagatgaa gttggtttta tatatataga ccctccgtat acagatatgc
    21481 aatactcaag atattatcat attcttaata cagttgtttt gtatgattac ccagaaatat
    21541 cattgagtag aggaactata tcaaaaggga tatatagaga aggtagatat caatcacctc
    21601 ttagtcaaag aaaacatgca ttaaatgaga ttgatttctt atttagtttc tgtaaggaaa
    21661 ataaaataaa tgtagcatta agttatgcat atccaataga tacagaaaaa caagcaacta
    21721 atagatatac ggctagtatt gatgatttga taaatgtagc caaaaaacat tttggagata
    21781 atgttaaggt agtacatgaa gaatatagtc atgcaaataa tagaaatagt gataaaaaga
    21841 atgtacttga atatttaata atgggtatac acaataagga gtaaatatgg ataaaattaa
    21901 taaattaaaa gagaggatag ctaatataga agcgacgaat aaaaatgata tatattcttc
    21961 taacttatat tggtcacaaa aaccattcaa tatatgtgat gaaattattg aacatttaag
    22021 tgaagatgga gatataatat ttgatccgtt tatgggatct ggggttacag ttatagaggc
    22081 tttgaaaaaa gagtataaaa gaaatgctat tgggtgtgat ataaatgatg caccaatatt
    22141 tatagtaaaa acattattag aaaaatataa tgtgcctaaa atagagaaag atattaaaga
    22201 gctaaataat aaattaagta aattatcaac ttactattca acaaaatgtc gtgaatgtgg
    22261 agaagtagtt actatatcga aaactatatt cgataaggag aataggaatg caaagtgtga
    22321 gctaaagtct atacattata aatgtaagga atgtaaaaat gactttaaag gtgaaccaaa
    22381 tgaaactgaa atatctaaat tctttattga tgaagaatta aagaatatag atgattttca
    22441 attcatagcc aattcaaggc ttgcagttgg cgaaggtgat acagtaagaa ctattttttc
    22501 aaatagaaat ttaatagtat tagataaaat attaggacat atagatgaat ataaaaatag
    22561 tgaatcttat gagcctatga agtatatttt attatctatt ttacatttaa gtaagattac
    22621 agatacacat tctaattctc agtggccttt atggattcct aaaaaagact gtgttgaaaa
    22681 aaatgtagta gaattattca taaagaatag taagaaaacc attaaggcat taaaactagc
    22741 atctaaatat ttatgtgaaa atagagaaaa tgtagaacta atcaaaaatc tagatagagg
    22801 gaaatttaaa ttgataaaag atggagttca aaatatagaa gatgatgtta tacctaatga
    22861 atttgtggac ttgataataa cagatccacc atatatggga caagttttat attcagagta
    22921 tatgcagtta tataaacctt ttatggattt ggaattcgaa ttagaaaatg aaatagttat
    22981 atcaagtgcc ccagctagag ataaagggaa agtattatat tttgatatga tgaattctgc
    23041 atttgaaaat atatctagag tattaaaggt aggtaaatat atgtgtatgt acttccatga
    23101 tagtaatcta gatgtatggg ataatataat agatataatg accaataata atttgaaata
    23161 catgggacaa attcatgtaa gtaagaataa aaacactctt aaaaacatac ttagtcctaa
    23221 gaagtcgtta aatggagatt gtataatttt ctttaagaaa gatttaaata taaactatga
    23281 tataaatgaa gtagataata tagaagataa gataaataat attgcacaag ctattataga
    23341 tataaaagga tacgcatcaa ctccagagtt atatgataat ggagcactag agtatataat
    23401 atcaaacgga aaactaaaag agatttctaa aaagtataag gatttaacaa gtatatttga
    23461 gcaaagattt aattgggata aggaaagagg ggtatggact gtataataaa taaaaagcat
    23521 ctatagataa ttatagatgc tttttattta tattttatag taaactaaat atttaataat
    23581 ttctaatttt tatacaatat gttaaaggat tggctatcca aagatattat ttaatataat
    23641 tttttagcat taactctagt taaatcactt ttagataatg aaacctgtaa tcttctgata
    23701 ggagaaccct tttctaaaca tctattgatc ttttcctgta acttcttacg aagaagagga
    23761 actacatctg ctggttcagt tgagtagata tgttcaactt ctatattttc tttatcaaca
    23821 actgctaata ttatagcttt atcattataa tatttattta gtacattatc agatatatct
    23881 tgaaagttcc aagcatgatt tttagaaact ttatattcat aaggattacc ttcactatca
    23941 taggcatcat gagcaccttc tcgtccacct tgctctgggt ttacttgatg gtttaattct
    24001 aatgcaacta ttaattccca aaacttatta cttgtaagta aatttttaac tcctgttaat
    24061 tcttcaagtt cacttgaagc tttataaata gcacttagat atttatcata aacaccattt
    24121 tgagctgatg ggattatctt acttgcattt aatctagact ctagttgatt agggctgaca
    24181 tttatcattt ttctggaatt ttgtatactt tgagtatact ttagtaaata ttcacacatt
    24241 atatctccag gtatagagta ataccaatca agctttccag ggtaaggaaa atcaacatca
    24301 tttatatgag caaatataac tgccttagct ttctttaatt ttgctatact tgtatccgaa
    24361 aaatcattaa acgtccaaga atggttagag ctacttttct tgaaatgttt atattcaaac
    24421 tcatttccga attcatcttt tgcatctcta gaaccactat gtccaggtat tagtatgtga
    24481 ttaagtgaat tagcagtcat aacttcatat attttgctat tagaaagtat atcatttact
    24541 ttatattctt taaaaatacc atcttgtata gcctttagtt catttatttt ctgtgttatt
    24601 aaaacatttg ttatattatc tcttatcata ttatcatagc tccttaataa attagtattt
    24661 ttagataagt catttttaat tagatttatc acataactat tgatgctatc atttttaaat
    24721 gctactaaat ccaataaatc tttatgaaaa tcatccttga gtcttatagt tagttttttc
    24781 aagctatcac ctcggatatt aattagatgt gccgtcgaga taagtataat taagcaggtt
    24841 tataaagtca aggattaaaa aaattaaatt atagatatat aaaagtatat atagcaatat
    24901 agataaatag ctaaactatt aatcgtgggt attattcttg cgattcgtct ccaccaattt
    24961 tgaaaaaaga atgatgaaat acttggaaat actagtcttt gttgactagt attttttatt
    25021 ttggagaaga aatccaccaa ccgtacccca accacagaaa atgaggtttt tttgggggta
    25081 tattggggtg catttaggct gtataaaaag tttttatttg tttattattt tatttgttta
    25141 ttattttatt tgtttattat gataataggt aacatagaat tttaatatct actttatgat
    25201 ataatatttt tataaatgta agacagttta agttgttaat tagtaaaaat atatattata
    25261 taagcttgat aggagttgag gtttgtaatg gatgaaagaa aactagaatt ttctattttt
    25321 tgcattgaag ctttagcaga aaaattgaat atagattcag aaggagttta taaacttctt
    25381 aaacatgata ctgatatttt agataactat ataatacctt gctatgaagc actacattct
    25441 caaagtaaag gttacatagt ggaagattta atgcaagtac ttaaagaaaa gggtgtgata
    25501 taatgattgt atatcatggt tcatattgtg tggtaaagac gcctagtatt accttttcaa
    25561 gagattcttt agattttgga aaaggattct atgtaaccag tataaagaca caagcaatta
    25621 actggacaga gcgttttaaa aaaagaggta aaaaagcata tttaaatagt tattctttag
    25681 atattgataa attaaaagaa aactgcaagg taaaaatctt cgatagttat aatttagaat
    25741 ggcttgattt tataatagaa tgtagaaata tgagtgatat atatatgaaa tatgacatta
    25801 taataggtgg tattgctgat gataagattt acaatacaat agaactgtat caagataatc
    25861 ttattgaaaa agatgaagct ttaaaaagat taaaatacta taaaccaaat gaacaaatat
    25921 gtataaccaa tcaaaatgta atagataaat atttaaagtt taggtatgaa gaggaggtat
    25981 aatgattatg gaagcaaata aaacattatt acaaatacta tataaaaaaa tcatattaga
    26041 attttccaaa caaacaggta agagcttgga agaaagtatg gattatttat atacttctga
    26101 aacatataaa cttataagtg agggtgtatc agatatgcac tgtaagggtc atatatactt
    26161 ggctcaagaa ttaatgttag aaaatggatt aatgtaccat aagggctatc caagataaat
    26221 aattaaaact agatacgaaa taaggataca atataatata aaattaattt gtatccttat
    26281 tttgattttg taattaacta tatgaaatca caatatttat atgtatttga tttttacaat
    26341 gaaaatattt tatccaactt ttctacagct tcaactttct tatcttccat aacttgagta
    26401 tatatattca aagttattga tatatcataa tgacccatta aaacttgtac agttttagga
    26461 ggaacatcat tctcaaaaag tctagtagca taagttttcc taagaccatg aaactttata
    26521 ggctctatat ttaagctatt taatatagac tttaaattcc ttaaaggtct tttatcatct
    26581 atagggatgc ctaattcatt gcaaaataca taattattat ttatatataa ttctccagcc
    26641 tttaatatat ctgtattttg atttacttta tgctctttca atttatttaa aacatctttt
    26701 ggaataggta cagttctgta agaatttgat gtctttggcc cttgctctaa tactttaagc
    26761 tctctatttc cagttttatc tataaaatat gttctttgaa gagttctttt aactgttaaa
    26821 ttagactttt taaaatcaat atcaaaccat ttaagtccta ataattctcc taatctaagt
    26881 ccagtaccaa gagcaagtaa aaataatacc tctaatttat gcccatctat agcttttata
    26941 aaactctttt gttgttcaag agtcaatatt tcaagtttgt tttctttttt ctcaactggt
    27001 atatttacca tagtacaata gttacgttgt ataagacctt gtttttcagc ttctgataag
    27061 caggttttca gtttagtgtt tatttgcctt atagtaggta tacttttatt ggaatcaagc
    27121 aatctattgt aatatttttg tatgtgagat actcttaagt actttaattt aacattgcct
    27181 aaatcagtat ctttaatata attacgatag atgctttcat atctttcaaa tgacttgggt
    27241 tttaaatctt ttattctata ttcaaataac caattgtaaa accaatgctc taaagttatt
    27301 ttatcatctg aaatatcaga gcttaaataa tattgttttt tataagcatc taatttatct
    27361 ttaacttcct tttgagtctt accataaaat tcttttctca gtggtttacc atcttcatta
    27421 tagccaatca ttatacggga acgccaacca tctttatatt ttccgataga gccttctcca
    27481 ttagctcttt tagttgccat ttttaaacag ctccttatat gatttatagt tatatccatc
    27541 aaagaatgta atacatttct ctaataagaa ttttataact tcatcagctt ctagtgtttc
    27601 atagaagttt agcattaatg tgttgtactc ttgtttatgt ttagctggta ttgatagaac
    27661 tcctatatca ttatttataa gtattaggtt tgaggtaagt ctagcagttc ttttgttgcc
    27721 atcatagaag aattggcaat aggacaacca taaatttatt attatagcct tttctaatgg
    27781 gttagttatt ttatttatga tatttatgtc atttacaaat aaatcattta atagagtggc
    27841 ttctatgcat tcatattgag tagtaccagc tatacctata ttaccatttc tgaaatctcc
    27901 aacgattaga gcttcatctt ttgcaactat tttatgtata gagcaaattg tatctttatt
    27961 taaagataag ttttctttgt tacttaattt tattacataa tcccatgatg attttatatt
    28021 tagtatttga ttttggtcac ttaatttatg tccgcctacg gttataccat caagtaatgt
    28081 ttgtacttct gggaatgtga aggggttatt ttctaggtta cccatatcat atacaaattc
    28141 acttatcatt cttctaatgt atgttaaagt atatttttta tctccatgag gtatattagt
    28201 agggaattta tgtgtgtatt ttggtatata tgatttattc atgattttag ctcctttcat
    28261 atctaatttt tatttattaa ctatgaatgt ataaaagtta ataggtaaac tcatattttg
    28321 aaaatgtgca atactaatat tcttcatcct catcttcaat aagaccatta gagttttctt
    28381 gtataaaatt ttcaacaaga ccatttatca cccaatttag atttttggag aagtctttga
    28441 agtcagagaa gtttaggtta attggtttta ttgtatctgc attatctgta atttcaaact
    28501 tttttttata gtctgttttt atatcataac cataattttc taatatgtga attactaaat
    28561 ccaactcttt atcaaatact tttaaattaa tattattatt tgttaaagta tctaaatcat
    28621 tttcttcaac aatgagttta tttatagcaa ttgataaagc agtagctatt tttcttatca
    28681 tatctaaact aggcattctt tgattttgct catatttagc tatagcatga actgaacaac
    28741 caatcttgtt ggctagctca tcccttgaca tatttatcga ttttctatat tgtttaatct
    28801 tttctccaat tgtattattc atcataattt caccttaaat atatcatcaa acaatgattg
    28861 tacttctaaa catgtaaagg agttattttc tagattatcc catatcatat actaaattac
    28921 tcataactct cttagtgtat gttagagtat attttttatc tccatgaaat aaattagtag
    28981 gggatttata tgtgtatttt agcatatatg atttattcat aattgtaact ccattcatag
    29041 ttttttatca ttgctttaga tagtattgat attattttat attttagtta gtagtttttt
    29101 tatttcttta agtgtatttt caagcttttc atttttttcc tttagttcgt tattttcttg
    29161 taataattta aagtaatcat tatttttaat agtaatattt tctttttgag atattaattt
    29221 ttttagtcga atattacttc cattatctgt tgagtcacta aagtaatcat agattgtttc
    29281 tatactttct tctgatagac caatatctaa taattcatca attgattgtt ttgtaagaac
    29341 atcattttta ataatttctt ttatgtcatc aggatgtaat gaaaaatcta attcagtatt
    29401 agatatacaa tctgaaagta tccaattata aacttgattt tcatttagtt ctaatttttc
    29461 accaagttta taatattggt ctttatcaga ccaggcacct agcaataaac ttgaggtaat
    29521 tctaatatca tcttccataa gatttgaaat agcatttaaa aattcatcaa aagaatagta
    29581 ttcttctggg aataaagact gtaaaaggta taaaacatct tcattagtca ttacttcatt
    29641 ttcaatatca aaataaaaga tagtcttttc aattttcaaa gctttagcga gttttgataa
    29701 ggtatctata ctaggtattc ttttatcatt ttcataattt attagagcat ttcttgagat
    29761 gccactaatt tcagcaagtt gaatctgcgt tagctctttt tgttttctta tcttttttaa
    29821 gttttttcca atttgcaata ttatcacctc gtataaagta tatcccaaaa gtacacaaat
    29881 gagaacagaa aaataatata ttcttattaa cagtacacat atatcgacat tataattaaa
    29941 atgtaaagtt tacgaatgta aactagaaaa aaggaggaaa aagtatggga atcaatttaa
    30001 aaatatttag gattaaaaaa ggtctaaagc aacaagaact ggcagataga gttggtatat
    30061 caagatatta tttatcaaac ttggaaacag gtaaggctaa taatccaagc aatgatttga
    30121 tgattaaact atctagagct ttagatacaa cagtagaaga attatttttt agtaaggagg
    30181 attaaaatat gaaagagaat aataatttag aggttttaaa gacattacaa gccactttaa
    30241 acattattgt aaagaatgaa gaagaagcac aagaaaaaaa gcaaggatat aaaattattg
    30301 aaaatgcgaa aagaaataaa gctgagctta taacatgtaa agaagcatct aagctttatc
    30361 ctataggaga atcaaaattt aggcagttat gtcattctaa aaacaaagga ttcccttgta
    30421 tatggataaa taatcgagta tacataataa aaaataaatt agatgattgg tttattgaaa
    30481 atgcaaatgg tatcaagtta tagtcttaaa gtaggtgttt gcaattgagc aataaagggt
    30541 ggataaagct ttatagggag ttgttggata aatcaatttg gaaagatatt aaaccagaaa
    30601 gaaaggtaat attaataact ctattactta tggctagtca taaagaaaat catgttttta
    30661 tagataataa aaagataaaa ataaagtttg gacaatttat ttcttcatat agcaaaatag
    30721 ctgaaagatg tggtaaagga gttagttatc aaatggttag aagttgtttg ttggttttta
    30781 aaaatgctgg ttttctaaca tacgaaacaa caaacaagta tatagtaata actattgaaa
    30841 actgggagct ttatcaagag aatatagaaa atcaacaacc taatcaacag actattaaca
    30901 atcagtcaac aacaatcaag aatataaaga atgaaaataa tattatatat agtgaaatca
    30961 taagcaaatt taactcaact tgtattgaac tttcaaaggt caagaaacta acagatacaa
    31021 gaaagaggag aataaattca agaataaaag aatatgataa agaaactgta ttagaggtta
    31081 taaatatagt tagtaaaaca agttttctga atggaaataa taatacagga tggaaagcaa
    31141 attttgattg gataatgaat tcaagtaatt ttactaagat actagaggga aattataata
    31201 gcaaggaaat tgtcataaat aaagagacag gaatatataa tccagatgac tcacaattaa
    31261 agtggtaagg aggttatatt ataaataatt caaacaactt aattaaaaat acagaagcag
    31321 agcaatcaat taaattacac taagttagtt ataaatcttt catatatttg ttttttaagt
    31381 atctctaata tcgtttaaat tacactaagt tagttgcaga tataccagaa aatatatata
    31441 aaattatgcg taaactatat tgttatgagt acaaataata tttacctatt cattaaaaaa
    31501 cttaactaag aatttaattt atattattga agtatcaata attttggaaa gtagaaaatt
    31561 ataccccaat aataccccaa ctacaaaaat agacaataaa aaataaccag aaaaattgct
    31621 tcaacatcat gaccaaatag ccatatacct atacttataa aaaccaacaa aacaatcaaa
    31681 taaccactta taaattctcc tcggtctcca ccattggaat ccacgaaaac atattgattt
    31741 aatagttaat atgtttttta ttttgaaaaa tcatatataa tataaattaa tagtttattt
    31801 aaaaatttca gtatgagcac aaaattatat aattattggg aattagttat aataagtaat
    31861 ttctagtaag aatgatttgc aaaaaataat aatttaaata caaaaaacta aaggatagaa
    31921 gtcataaaat taacttttaa cctttagtta tataaataaa aaattagtta ttactaaatt
    31981 aaaattagat ttttaaagaa cccaatatct ctattatcca tatcaccaat taacatgctt
    32041 ctatcaagta ataaattata ctcctcacag ctagtttcag gcaataaagt acatgaatgg
    32101 catgctccta gatttaaaga atttctacct tgaccagtac tatcaataca tattgggtca
    32161 ttagaacacc aaagagcacg attaagagca ctatttaata tagatggtaa tgaatcatgc
    32221 ataccttgcc taactaatcc tcctaaagta ccttcagagt caccacttgc agtgtaaact
    32281 aaaattccag acataacttt ttcattttcg gaattattac aatatatacg ttccctaagt
    32341 gaagaggtag catatccaca aacaaaacta agttctctta taattaaatg agctaaagta
    32401 tgtagtaata agaatttagg tgtaattttt ctataagctt tgtcattaac attaatatct
    32461 ttattatatc tagaatttaa aatagatgca ttatcctgta ttttaggatt agatgaaatc
    32521 cagcttttta ccatatcatt atttatctct atgaaaatac cttcacctct aacctcagat
    32581 gcaggataaa aagatttttt acaatctcta gatcttaaac ttataacctt agtatcatta
    32641 cagctatcat ctatatcttc ttcaccaaat atattattat caggtggatt gactctacta
    32701 aacccagtta atgcccttac ttctctaagt ctttttacta acactatttt actgaagttt
    32761 ggaatatcat aatcagatag attttgttct tctattgtaa agtcattagt ttttttagat
    32821 ttagcatcta tatttccaat taaagcctca tattcttctt ctctatattg atttctagtt
    32881 gttgttgatg gtgaagaatc ttcaaaaggc aataaatctt tcaaaactat ggatacttta
    32941 tcagtatcaa aacctgtttc tttagcaata tcactggcaa agtcctcaaa atcctcatcc
    33001 ataaagcgtt ctaacttatt ctttttaata gcacgatcta agaatttctt aaatgtatca
    33061 taacctttac tagatagcac tttagaatta agagtatcag agtatggtgg tataataatt
    33121 gaactttcta tcttaggata atatacatta gaggcacctc tttgagaagc aaatgaatat
    33181 ttagtgcatt tatttacttt tttattatac caaggcgaat taccagagca tttaaacatt
    33241 tccttatatt cactaggtac accataatca ttagatttag aagattttaa acgcataaat
    33301 gcgcccttca tattagcttt agctccacag ctacattcaa gttctatacc ttctaatcct
    33361 aggttacttt ttccagtctt cattaatatt tgaggatttt cacaaatttc ttttcttcca
    33421 gatttatttc taaaatgaac ccattttacc catggaaaat catcaatatg accatgttca
    33481 caaatagtaa ttataccagt aggtactaat tgtttagtag acttttttgt gctacaagta
    33541 aggcaaatag gtattttcat aggtttatct ttatgagcat ttttataaag tttctcccaa
    33601 tcttctatag gcataaattt tctacatttt ggacaaaagt accatctagg aaacctaaca
    33661 aaaggaacag aagatctatc atctatagtt ggaggtgttc ttagttcatt aaccctcaat
    33721 gtagcctgaa gcctatcatc ttgtatttta attgtattag tccaataatt tggatgagca
    33781 gtcattaatg tttgatcaat aaaatctagc atagctcctg gtccaaaaac acttgtagct
    33841 tgatttattc ttaaattatg agttttatca tctaatttta tagacatatt aattttcctc
    33901 caattctata atttcaacag tacattgttt atcaacattt ctcatagaat ttaatgtttg
    33961 tttttcattt gtaataacat ccttatcata aggaattaaa agattattat atattttatt
    34021 atcaaattta ctagggcgct tataataatg caataaatta tctttatcag atatttgagc
    34081 tttatcatgc cataaatcca ttataatatc taattcagat gaaatctcag atttaaattc
    34141 atttactata ttatcatcag cattttgatt attttctcta aatatatcga tagatctatc
    34201 tattatgaag ttttttaaat cttctacacc atccatattt tttgaaaatc gacttgcaag
    34261 atcattagct tgaagtccta aaatatgtct aatcatactt attaatactg catgcatgcc
    34321 tctttctcta gctggttcag agaaaggtgt aatactagta ggttcaacaa atttatagaa
    34381 tgattgatga taactaaaga actgttcata atgagatcta tctcttgttt ttgtaggatt
    34441 atataatgta aatataagtc cttcattact tctaccaact ctactgctag cttgtatgta
    34501 ttcagatgtt aacttaggct gaccaactac taccattaaa tttaatctat caacatcaac
    34561 accaactgaa atcatattac tagctaatag agtatcgaca gttaaattat tagaagtaag
    34621 agtattagat agttcatcta aagttgtatt tatcttagta gaagtaagtc tactagtaag
    34681 ctctctttgt tcatttaggt tccttaatga ttgataaggg tgggctgaga ctcttttaca
    34741 taatctttcc atgttgtctt ttatatcgtc atttaaaagt gtgctacact tacctaattc
    34801 tctgatacta ttaaaataag atactagtgt ccaatattta tccaataaat tcaactctac
    34861 attctccatc attttaagtc tttggagtaa tgctgacata agtctaacct ctacagtaac
    34921 ttgagttttt ccagatggaa taacacctac atatagtcgg ccacgttttt tacttaaagg
    34981 catctcttta acaaagaaag agttactaga tgttaatcct ggaggtggaa actgagctac
    35041 attacgatta tatagatttt taacttgatt ttgtgctttt cttatagtag cagtagaagc
    35101 tataatctta ggttttatac ccttatagct acacatagca tcaagtgcag tttcatataa
    35161 acctaccata gtacctaaag gtccagaaat aagatgaagc tcatcttgaa ttattaactc
    35221 aggagataag ttgctattat catcatctaa agcaaaaaga ctagctactt ctgtagtcca
    35281 agttatcatt gcaaatttat caactgtacc aaataaaagt gttggagggt tattataaat
    35341 ctcctcatct acaacttgta taggtaacat atcttcaaat gaacaatctt cttcaggaca
    35401 gcataagtag aatttcttag gtctagattt aattctatag ccccaatcac caacagcttt
    35461 tttagaagtt ttatcccatt ctttaacaag ttttgttcca caccaaggac attttagtaa
    35521 ttggaattta ttatattttg attttaaatt atctaactca tattcactat ttatactaga
    35581 tctaaactta atgttattac ctgttaagtt attaagcatt tcttctgcct catcattaaa
    35641 gtttggagtt tgatcactcc caatccacaa accaatagaa atctcttttt tcccaagttc
    35701 agacttattt tcttttctaa taatttcaca ggcacaaatt aatatacttg ctcttatgaa
    35761 ttgttgtgca gccaatagcc ttagtgtata tctcattatg atagctgtac cgttaccatt
    35821 ttctttgttt cttattcttc ttaagaagat agtaaaagca gatagaccta agtaggcctc
    35881 cgttttacct ccaccagtag ggatccaaag aaggtcaact aaatctctat gtttagagtc
    35941 aggattttct atcgaagata aacatgtaag tatataagca agctggaatg gtctccaagc
    36001 agaattatca tttgagtaat ctatatagtc aaggttatta gatccaggga gtctatccgt
    36061 aatatgtata gaatgacatc tttgcataag tatagctcta ttcattagag aaaatgctct
    36121 gtatacttca tcattagact taattaaatt tataccttct agcattctag acttagtatc
    36181 tctgcagttt tttatatgct catatgctac atctttaaaa acatcactaa gatcttttga
    36241 atcttcttct agatcatcta tccaaatatc ataagcatca acaaacttat aaagaaaata
    36301 tattttctta tctttactaa gatcagagat atctgataaa tttttcatag acagtaattc
    36361 tctagaaaca tcacttaatt catctacatt aaagtccaat ttaggtactt caaaatgagg
    36421 aataaactca gtacaaagtt ttattaaagc atcattttga atatcttgat ctatagctac
    36481 accatgacca gtagcaaaag tttttttatt tcggtaaagt aagtctaaag atatttcctc
    36541 agaatctaac tttttaaaag tatttactga tccagctgta aaaatatcta taaactctaa
    36601 atcattgtta tcttcagcta ataccattag ttcaggctgg aaaatagtat tattatattt
    36661 cttaggccca gattgactat taaccagcat acaagtaata ctaacaaagt catttgaata
    36721 aattctttta gataaaacta tatctaaatc tttagatata ggttttttaa tatagttttt
    36781 atccatatct attgatacta ttaagttttc atgtggaaca cgaacataac caccagtctt
    36841 attctcatca tcagggcatt ggttagctaa cttgtataaa acaaatttag cttcagcatt
    36901 atcaagtaaa tcatgctttt ttagtaaatt ataaaccttc tcagatgtta gtttttgctt
    36961 aagatatagg aatgatcccc taagttctac atattcatta attgtattgc tagaatagaa
    37021 gtcgtcacca gtatatttaa catgacagtc tgtatataaa gcatttctat aagtagcata
    37081 atttaccctg aaatttatta cttctgcatc tttttttagg aaaaaagtca tacccataga
    37141 agaaggtaaa gtttcattag acatacttat agctctatct agttcatttt gtgtatcatc
    37201 tgatgtaaat acattatctt caccatcata ttcagatgta ttacttacat caatattgtt
    37261 attaagtgga tataaaatac ctgttgaata acgctctatt ggactatctg ttattatttc
    37321 atattttata ttttcacaaa gatcttccga accaggtcca agaagttctt ttcttacaag
    37381 atctagggtt ttatctctta gttcactaca attcatattt acctcctatt tatttaataa
    37441 acactatata agtctaaaac tttgttaaca aggtggggtg acagatactt atcacaactg
    37501 tgtatttctc gtatttgtac aggtgtaaca taatcatttg tattcccaag catacttaaa
    37561 acaactgcag acacttcatc tttaattata tctgatttac tgctaaagaa attatcagat
    37621 acaaatgtat ctatagattt ttttatttta gtgtgcaaag agcgtatact tttagtagca
    37681 tcatatacct cggtatgatc taatttagaa tctataactt taaatatctt ttcgaaagtt
    37741 tttaaatata taggacctat taatctttta tttgttaccc agtttttgat tgttatagga
    37801 ttaatctttt cacctaaaac agctaaactg tttgatacat cattataatt atatttacta
    37861 taagatatat agtttttaag ttctgatttc catgaattag ttaatttata atcatcaaga
    37921 taatgagatg gcaataaatt aagttctaat agcttttgta taacattaat aactaattca
    37981 ccctcttcat ttaagtaatc atcaacaaat aaaaaccagt cattaacttt agcctctgaa
    38041 agtttctttt tcctaacact ttttttatcc caatctattg aataggctat tgcctttttt
    38101 gtacagaata tatactcatt attatcagaa atcatgaatg atgtaacatg ttctttttta
    38161 gagttgtaaa tcccttgatt tatagataga gataatataa tttcatttaa atttatatca
    38221 aatttataat tagatatata ttcatccata gaatcatcat cagaaatagg actatcggta
    38281 gaataagctt ctaatttatt ggtttcataa gttggtttaa aaggttttat atcaagtaag
    38341 ttatatattt tattattttt ttcaatttta tttcttgcag aatttatttg attcatatag
    38401 taattatatt tggataattc actattatat aaatacagtg taatatttat aatcaaatca
    38461 ctatttaaaa tatctaaata tttcaaatca tagaatccaa acataataag attttttatt
    38521 gtttttgaat ctttaaattt ttcagcagaa ataacttgta aatttatatt tctagatttt
    38581 aataaatttt taagtataat agcttcatat tgtttttgta gcaatataac aggtgtattt
    38641 ggtgtaaatg ttttgtattt atatgaagat actaatttat ttagtgtatt gaatttggta
    38701 ttattatcag ataattcatt tataattctt tcaaatttga ctataacatc atcaaataat
    38761 gcacttaatt ctaaatctgg gctagtactt gaaattaaat attttatatt ttctagcttc
    38821 gctgaaggag ttgctatatt taaatttaaa agctttatag tttcttctaa aaaatctagg
    38881 ttaaaaggtg tattttctaa gattttaaga agagagtaac tacacatcat aagtgagttt
    38941 tttatggaaa tatcaaaatc atacttagat atgtaataaa gtgattttct agcctgatgt
    39001 atcaagtcag agatattaga atcaacagaa gtagtacata tatttttatt tataagttta
    39061 tctagccata atttctgaaa agtagaggta ttttcaaaat cttggctata ggttaacaga
    39121 acatctttag accaagagta tatatttaga ttatctatat ttggtaaatc taatatattg
    39181 cttatatttg aatttgaggt tacaatattt acactttcta tcctttttct atttaaaatt
    39241 ctttcaaaat catttatatg cttataaatt ggataatcac ctattataaa tacatttttt
    39301 atagacttat cagatttaat aatttcaaat gcactagaaa tattagttgt aaatttaagt
    39361 aaaggtattt gcttagcgtg atttccagga taatcagttt cggcaccagt tgatgaaata
    39421 tatgaacatg gaaatagttc agtaaactta taagtattat taatatcttt tattttaata
    39481 ttactaacta agtcagatat atcatcttta caaggcaata taatcagtgt agaatcattt
    39541 attactccag tcatattttt taagtctgaa aaacctaaag atgatagaac attctttgtt
    39601 tgaaaagtct tagatgctgg agttttgccc atagaattga cttctaaatg accattatag
    39661 gttataagca tataagccga gtcaataggt ataagtgttg tatccgtttt atttttatgt
    39721 cctaaagtta tgtgtgtatt atttatattt ttaaatataa ctatttgacc cttgtagagc
    39781 aggggcatac ctttatcgag ttttgtaaga atgtgattat ttgggtttaa tgtattattt
    39841 aagtatgagt ttatagctat attcaacaaa taaatagcat atatgtattt gtcattataa
    39901 ttgataatta agttattatt tttttcattt ataatataat ttaatacatt aatattatct
    39961 tgataatact tagaatttgt tagcctttgt ttattaaaat aaatttcaca tata
//
LOCUS       DAMJWU010000026        40007 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMJWU010000026 DAMJWU010000000
VERSION     DAMJWU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.026"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            343..1203
                     /locus_tag="PC460_001513"
     CDS             343..1203
                     /locus_tag="PC460_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2600402.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(1442..1999)
                     /locus_tag="PC460_001514"
     CDS             complement(1442..1999)
                     /locus_tag="PC460_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2600403.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(2189..3076)
                     /locus_tag="PC460_001515"
     CDS             complement(2189..3076)
                     /locus_tag="PC460_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2600404.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            3195..4394
                     /locus_tag="PC460_001516"
     CDS             3195..4394
                     /locus_tag="PC460_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF2600405.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(4588..7143)
                     /locus_tag="PC460_001517"
     CDS             complement(4588..7143)
                     /locus_tag="PC460_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2600406.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWSLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(7140..7826)
                     /locus_tag="PC460_001518"
     CDS             complement(7140..7826)
                     /locus_tag="PC460_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600407.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGELL"
     gene            complement(7903..8811)
                     /locus_tag="PC460_001519"
     CDS             complement(7903..8811)
                     /locus_tag="PC460_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2600408.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(8813..9484)
                     /locus_tag="PC460_001520"
     CDS             complement(8813..9484)
                     /locus_tag="PC460_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600409.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            9746..10810
                     /locus_tag="PC460_001521"
     CDS             9746..10810
                     /locus_tag="PC460_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF2600410.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            10968..11654
                     /locus_tag="PC460_001522"
     CDS             10968..11654
                     /locus_tag="PC460_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600411.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYKDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            11644..12675
                     /locus_tag="PC460_001523"
     CDS             11644..12675
                     /locus_tag="PC460_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2600412.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITGDLKLIALLL
                     IYTMVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            12757..13422
                     /locus_tag="PC460_001524"
     CDS             12757..13422
                     /locus_tag="PC460_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600413.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            13435..15852
                     /locus_tag="PC460_001525"
     CDS             13435..15852
                     /locus_tag="PC460_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600414.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSST
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(16033..16824)
                     /locus_tag="PC460_001526"
     CDS             complement(16033..16824)
                     /locus_tag="PC460_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2600415.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(16857..17444)
                     /locus_tag="PC460_001527"
     CDS             complement(16857..17444)
                     /locus_tag="PC460_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2600416.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYSNSKSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(17621..19012)
                     /locus_tag="PC460_001528"
     CDS             complement(17621..19012)
                     /locus_tag="PC460_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF2600417.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(19202..20500)
                     /locus_tag="PC460_001529"
     CDS             complement(19202..20500)
                     /locus_tag="PC460_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF2600418.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGYPLPIPYIMPLKETLCMS
                     MSRGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(20857..21732)
                     /locus_tag="PC460_001530"
     CDS             complement(20857..21732)
                     /locus_tag="PC460_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF2600419.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(21925..22611)
                     /locus_tag="PC460_001531"
     CDS             complement(21925..22611)
                     /locus_tag="PC460_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF2600420.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(22660..23193)
                     /locus_tag="PC460_001532"
     CDS             complement(22660..23193)
                     /locus_tag="PC460_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2600421.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(23237..23869)
                     /locus_tag="PC460_001533"
     CDS             complement(23237..23869)
                     /locus_tag="PC460_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2600422.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(23959..25038)
                     /locus_tag="PC460_001534"
     CDS             complement(23959..25038)
                     /locus_tag="PC460_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2600423.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(25171..26517)
                     /gene="pepV"
                     /locus_tag="PC460_001535"
     CDS             complement(25171..26517)
                     /gene="pepV"
                     /locus_tag="PC460_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF2600424.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(26586..27866)
                     /locus_tag="PC460_001536"
     CDS             complement(26586..27866)
                     /locus_tag="PC460_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF2600425.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(27993..28253)
                     /gene="hfq"
                     /locus_tag="PC460_001537"
     CDS             complement(27993..28253)
                     /gene="hfq"
                     /locus_tag="PC460_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF2600426.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(28367..29302)
                     /gene="miaA"
                     /locus_tag="PC460_001538"
     CDS             complement(28367..29302)
                     /gene="miaA"
                     /locus_tag="PC460_001538"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF2600427.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(29340..31307)
                     /gene="mutL"
                     /locus_tag="PC460_001539"
     CDS             complement(29340..31307)
                     /gene="mutL"
                     /locus_tag="PC460_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF2600428.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(31318..34161)
                     /gene="mutS"
                     /locus_tag="PC460_001540"
     CDS             complement(31318..34161)
                     /gene="mutS"
                     /locus_tag="PC460_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF2600429.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(34322..35056)
                     /locus_tag="PC460_001541"
     CDS             complement(34322..35056)
                     /locus_tag="PC460_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600430.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(35025..36038)
                     /locus_tag="PC460_001542"
     CDS             complement(35025..36038)
                     /locus_tag="PC460_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2600431.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(36060..36830)
                     /locus_tag="PC460_001543"
     CDS             complement(36060..36830)
                     /locus_tag="PC460_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600432.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(36805..37095)
                     /locus_tag="PC460_001544"
     CDS             complement(36805..37095)
                     /locus_tag="PC460_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF2600433.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(37226..37325)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      37945..38034
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            38087..38263
                     /locus_tag="PC460_001545"
     CDS             38087..38263
                     /locus_tag="PC460_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600434.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(38378..38734)
                     /locus_tag="PC460_001546"
     CDS             complement(38378..38734)
                     /locus_tag="PC460_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600435.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            38990..39903
                     /locus_tag="PC460_001547"
                     /pseudo
     CDS             38990..39903
                     /locus_tag="PC460_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
BASE COUNT        13634 a         6213 c         4647 g        15513 t
ORIGIN      
        1 taataaatcc ttttgataac atataaaagt aagataaatt tttccattaa ataccttata
       61 attatacaca aaatctctat atacgttaaa cagtatagag aaaattttca tagaaccatc
      121 tttatagggg atactttata aaagaataaa aaaattataa tatataaaaa cttcatagga
      181 gagtaacaac atcagtaaaa atatgaaaac actatataaa aattcaaaaa tattaagaat
      241 aatgaaatac ttgaaattgc ttatatcaag aaattttata taatataaac tggtcaaata
      301 tgttataata ttaaattgtg acaagttcat ggaggtgttt atatgaaaaa acaaaagtcg
      361 ccaattttac ttgaaatgct tggactattt tttggttgta tatcaatgag tattggtata
      421 aatatgtttt taaaaccaca tacaattgct cctggaggac ttagtggtct atcattagta
      481 ctaaacaagg ttacaggact tccagtatca gctatcatgt taattatagg tgtcccatta
      541 gtaatacttg cttttagaat tatgggtaca aaaaattcat taaaaacttt atttggaaca
      601 gttgtatttt cagctatagt tcaattaaca gacccattat caaaactaag atttactaat
      661 gacttattat tgtcttctat tgcaggagga atcctagttg gtataggttt aggaataatg
      721 tttaaatcag atgcttctac tggaggaaca gacttaattg cactcatact tagtaagaaa
      781 ttcccaggaa taaaagctac aaaattcatg tcatgcctag atggaatggt tgttatatct
      841 tctggtatag taaatcgtag tttagaaact gctctttact ctggtatagc actttgtgta
      901 ttaataaaaa tagcagatat gataatggaa ggatttgacc attcaaaagc attttttatt
      961 atctctgatg agcctgaaag tttaagacaa gctataactg aagaacttga tagaggtctt
     1021 actattcttg atggtaaagg tggatacact agagaaaata aagaagttct attagtcgta
     1081 gtttctaaaa aacaagaact ttatctaaag agattagtta aaagaactga ccctactgca
     1141 tttgttattg ttacagatgt acatgaggtt cttggagaag gatttaaaaa tttagagaac
     1201 taataaaaat taatatttta agtatatata ctattttaat taaaatacga tttattcaaa
     1261 aagagtgtct cataaacttt ttagttcatg agacactctt ttttgtataa aatcaaatgc
     1321 attttcagta ttttaacaat aaaaacagga ttatctatat tttgaggcaa ccactttatt
     1381 gaaattttat ttagaaaaca atcattacta aataaccagc atagatatta aatgattata
     1441 tctattgttt tttcaattca tattgataac aaggaacatt tttcacagta tttccagtat
     1501 gaattttttt gatatacttt cctgtacatt taaatcctaa taaatctaat gattttgtag
     1561 aattttggtt ttcttcatag gttattgcaa ttattttatt taagtttagt tctctaaatc
     1621 catactgtat tactcttttt gttatctcag tagcaaaatc atgtccccaa tagcttggat
     1681 ttatacatat acctatttct gcttctaatg ctttaggatt tatattatta aaccccatat
     1741 tcccaataac tttattgtct cttttatata ctatagcaaa gtggccaatt ttcccttttt
     1801 tatatggatt tagaaaaaat gattttataa actttcttgt atcttctaaa gatttatgct
     1861 cactaaatgg taaatgtttt actactattt tatttttata acagtcaaaa aaatcttcaa
     1921 catcatctaa tgatgcaggt cttagtatac aatttctagt ttctaaggtt ttcatttgag
     1981 gatattcaga atcagacatt tctaatcccc ctttatacat taatctaaat atactgttca
     2041 tatttaaaat acgatattaa ttatataaat gttctcaaat taaccttatt tttattttta
     2101 ataaaaacta attactatat gaatctttca aatttacgaa tttatctcta tttttatatt
     2161 aaattacttg tgaaatatgt attctaaatt atatattatt agatatcatg cttaaaaatt
     2221 ctttggttgc taaggataaa gatatatctt ttaaatggca gtaacctata tttctcttag
     2281 gtattggctc ttttatatct aatttaaaca atttctcatt ttccaaataa tctatgctaa
     2341 attcttctat aacacaagac actccaagat ttatatacgc aaattctaaa agtaattcat
     2401 gagaacccaa ttctatatcc gggtttagtt ttataccttt tgataaaaaa tattcattta
     2461 cataaagtct tgaatttgct ttattttcta gcattattaa aggcaatgtg tttaattctt
     2521 ccaatgatat tttcctacct ttgtattcta tgtaatcatt tgcacataca aatatatcat
     2581 gtatttcaat acactcaaca atatttagtg tatcatcttc tattggcatg tttataattg
     2641 ctatatctat attcccatct ttgagtaaag atataagttc tctactcgtt ctatttattg
     2701 tagatacatg tatatgtgga tacttcttat gaaaaatttc taaatatttt aacaagaaaa
     2761 aacgtgcagc tgtatctcct actcctattt tcagagaacc gtattctaat tttttaaatt
     2821 tatttattct atcttctcct gatgatatta actttaatgc agtagtgaca tgttcactta
     2881 gaattttccc ctctggagtt aattttactc ccttattgtt tctataaaat aataaagtat
     2941 ctaattgttc ttctaattgc tttattgact ggcttacagc tggctgtgac ataaacaatt
     3001 ccttagcagc aagagaaaaa ctcttattat ttacaactgc attaaaaacc ttataaagtt
     3061 caagttttat attcatatat aattactcct tatgtcaaat attagtatta tttattttac
     3121 ttataattat atttgaatta taatatacat ggtactaata tgacaatatt aattctttag
     3181 gaggatttta gataatggat agattagttg gaactgtttc aagaggggtt agagcaccta
     3241 ttataagaca aggagatgac ttagttaaaa tagttgttga ttctgtttta aatgcgtcaa
     3301 aaagtgaaaa ctttgaagtt agggacaaag atgtaattgc cgttacagaa gctgttgtag
     3361 ctagagcaca gggaaattat gctcacgttg acaatattgc aaaagatgta aaagataaat
     3421 ttggagatga tactgttggg gttatattcc caattttaag tagaaatcgt tttgctatat
     3481 gtttaaaagg aattgcaaaa ggatgtagaa aagtcgtatt aatgttaagc tacccttctg
     3541 atgaagttgg aaatcactta ataagtattg atgaacttga tgataaggga ataaatcctt
     3601 ggtcagatgt tttaacagaa gaaaaataca gagaactatt tggatataac aagcatacat
     3661 ttactggtgt agattatgtt gattattata agaatttaat caaggattgt ggtgcagagg
     3721 tagaaatagt atttgcaaac aatccaaaaa ctattcttaa ctatacaaca agtattttaa
     3781 cttgtgatat acatacaaga caaagaacaa agagaatttt aagacaaaat ggagctaaaa
     3841 aagtatattc acttgatgat ataatgacag caagtgtaga tggtagtggt tacaatgacc
     3901 agtatggact tttaggttca aacaaagcta cagaagaaac agtaaaatta ttccctataa
     3961 attgtgatga agttgtaaac aaaatccaag gtaatataaa agaaataaca gggaaagatg
     4021 tagaagtaat ggtttatgga gatggagctt ttaaagaccc tgtaggtaaa atatgggaat
     4081 tagctgaccc agtagtatct cctgcttata caaaagggct agagggaact ccaaacgaag
     4141 taaaattaaa atacttagca gataatgatt ttgctgactt atctggtgat gagttaaaag
     4201 aagcaatttc aaaatacata gttgaaaaag ataataaatc tgatgattta acaggaaaca
     4261 tggtttctca aggtactact ccaagaagat taacagacct tataggttct ttagcagatt
     4321 taacatctgg aagtggagat aagggtactc ctattattta catacaagga tatttcgata
     4381 actatacaaa atagtatgtt ttaataagat ttaatttttt tggattatat atattaacat
     4441 gaaaatttat taaaacctct taatagatat gacttttcaa gagttatatc tattaagagg
     4501 tttatttaat gttcacagat aagatatatt taaataattt aaacaagatt gtcagaaagt
     4561 tatataatct taaaaattta tataactcta attcttattt atttctgaaa taatatcact
     4621 cttcaaaatt tgtcttactg gaataattac agcaactata ctaatagtga tatttaaaat
     4681 aatcatacct atgtatagag gtgtatttat tttaagaatt ggatttatat ataaatatac
     4741 acgttttacc ataaaataaa tcataaattg tccaataaca cttccaatta ccattataat
     4801 acttgcatag aatccatata aaaaaccttc tcttatcatc ataagtaaaa acttattatc
     4861 cgtaataccc atagctctta aaattccaaa ctcatgtctt cttgaaaaaa ttaagtagct
     4921 tactgtattg attatatgga acaaacttat tgcaagaagt acaaatacaa taccatatat
     4981 aaataagagt ttttgattta agtaggtatt atttttttca atagcaatgg tataatctgt
     5041 tacgatacat ctttttacat cacttacaac actttttatg ttctcagaca attcctcact
     5101 gttactatct tctgtctttt ttatacttac tgaattataa ttttcgatat caaagttatt
     5161 tttcatttgc ccattcgtca taactatatc cataccataa tcaccaatga aatatatgtt
     5221 atttgccatc acacgtttta cagtagcagc aactacaaat tcttttgttg tataatcatt
     5281 cttattttct tctttaaatt taacaatttc atctgttggt ttcattgtct ttggcacttt
     5341 aacttttatt gtatctcctg gatttatatc aactgcatca tacatcccag ttccatccat
     5401 aggtagtcta actataacct tattattttt aaccatatca tgggtattaa tctccccatc
     5461 tataatataa gaatttaatc cttctaacat accattgtta taaccataaa tatttgtttt
     5521 taaaagatag ccaccttcat tttgctttgt acaaatacca tcaaaaaaag tctttatcct
     5581 gtaatctttt tcaagaggtt taaagaaatt tttccaaaat agttgttctt ctttaatttg
     5641 catagctcca aataaatatc ttactggata gacttttgat attccctcaa cattgtttaa
     5701 tcttgaaatt ttatcttttg gtatacctac atcaaaatct gttgtttgca tagttatttg
     5761 gtagtcagaa tttaaaccat catcagcttt cattgtaagt tcattattat ttttagtaag
     5821 ctgtatagag taactagaac atagaaatat aattccacca agtgataatg aaaacaaaat
     5881 tccaataaac attccctttt tttgagtcat gtatttatga gataatactt ttgtcatgtt
     5941 agagtgtttt acactttcta atattctctt tttcttatta gtaatcttaa agtttttcat
     6001 agattctata ctagagtatt tattcaactg atgtacaaca cgtacagcaa ttaaaattaa
     6061 caacaataag ataaataaaa agcctaaata aatagatttt tcacttataa aaaatgctcc
     6121 tggaacaata tcactactca aaaatatatg tggaaattgt tcatatattg tagacgctac
     6181 tccaatacct aagaagcaac ctataggaaa tccaacaata aaaagtaaaa ataactctaa
     6241 aaatagtaga aataaaagtt gtttattatt agcgcctaat acttctaaaa ccccatattg
     6301 tgatattctc tgaagtattg aaacatggaa aatactatat actgctgctg caccaaacac
     6361 tccaacaagc aatataattg catttgtatt tagttctaaa ttttttaaga actcaaatat
     6421 attatatttt tctatatttt ttggtggttt tactccaaat tcaactgaaa gagaccagtt
     6481 tatataagtt ttttcttttg atatgttgaa ttttttcata aatttataat tttgcttttt
     6541 aatattttta ttttcatcat attttacata aactatataa gaatcctttt ctttaggctg
     6601 aaaataatcg tttacaaatc cctccagagc agtttctggg tttgaaagac ttgataaaat
     6661 accacataac ttatagattt taccatctaa attgatttct gttccaattt ctttgtttac
     6721 atttaaattg tttagagaat atgtgtcaag tactatctct ccatctttac taggaagagt
     6781 tccttttaat aattttctac ctgtcatgtc taaatatgaa gaatcagcat ttaaaaattc
     6841 tataataaaa ggttcatcta tctgattttt aggttttgta acgccaagtc tatctacagt
     6901 gtattccttt gattttttat tttctttaat tttattaagt tgttccttat ctactttata
     6961 gaaataatga taatctccac tatcttccct tatcttttca attctacttt tgtctgcact
     7021 atgaactaaa gaaccaattc ccgtcaataa tgctactgat agtactatgc ttaaaactat
     7081 agatatagat tgttttttat aatgctttat atattgaatt gaaaatttta ctagagacat
     7141 catagtaatt caccaccagt attaaccaca actccatctt caatatggat aattgagtcg
     7201 catacttgtg ctatattttc attgtgtgtt accataagta tcgtttgatg gaattttgca
     7261 ctactttctt ttaataaaca taccacttcc attgtagttt tactatcgag atttcctgtt
     7321 ggttcatctg ctaatataag cgcaggttta tttgccaaag ctctagcaat tgctaccctc
     7381 tgttgttgac caccagatag ttcgcttgga aattttaaac gcttatccca aattcccaat
     7441 gtcttcatca gcatttcaat atattcatgg tctatatggt ttcctttatc taatgtaaca
     7501 ggaagagcta tattatcata agtgtttaat acgggcatta aactgtagtt ttgaaatata
     7561 aatcctatat ttcttcttct aaacactgtc aaatcttttt ttcctatttt agatatgttc
     7621 tttcctttaa ttattacatc acctgaggtt ggattatcta atccacctat aatatttaac
     7681 aatgtgcttt ttccagaacc agaagttcca actatagcag taaatgtatc tcgttcaatt
     7741 tctatactta ctccatttaa ggcttttact ttagtctcac cttttccata gtgttttact
     7801 aaattattcg tcactaatat actcatttaa tttctccttt caatacagat atatgttcta
     7861 taaaatattg taagtgacga taattacact catcctacaa aattagttta aactcttaca
     7921 attctgtaag aagatactaa aacaacatcc acttcctagt ttagattcta ctataatgtt
     7981 tccattttct ttttctaaaa ttaatcttgt aagatataga ccaatccctg tgccattttg
     8041 gttttcaaca tttttacttc tgtaaaatct tttaaaaata tcatttagtt ctctactatc
     8101 aatacctatt ccctcatctt taatagtaat cttcgtatat agttccattt ttattacaga
     8161 aatagtaatc gtgctatctg atggtgagta ttttattgca ttttctaaaa catttacaat
     8221 tgcttctatg gtccactttt tattatgaac taattttatg tctgaaaatg gttcgagatt
     8281 tatccttata tttttagctt ctgctttatt aaagatagta ctgatacttt gaataagagt
     8341 atctttaatt agtagttctt ctacttcaaa ttctataact ccatcctcta atttagtcat
     8401 tttaaataag gattgtaaca gccagtcaat cttaaaagtt tcatttttca tcttttgtaa
     8461 aaactccttc ttctgtaatg tggaaatatt tatattctcc agaagttcac aataaatggt
     8521 tatatttgaa agtggagttt ttaattgatg tgacatattt gatattaaac ctttaatcgc
     8581 ttctttttct gactttgact gttctacctc tatttctatc atatccttaa ttttttttac
     8641 ttggtgagaa atttttgacg ctcttgtatc ctttgtatct ggataagtaa cttctttacc
     8701 ttccaatatt tgatttaaca tcaaatttaa ttcatcatat ctttttcgat ttattttagt
     8761 agatactata aatataacaa ctgtacatat tataaaggct aatataaaca tattattcta
     8821 cctcattcca taaataaccc attccataag ctgtttttat atattttgga ttagatgggt
     8881 catcctctac ttttactcta agtcttctta tatttacaga tacaatatta tcatcaacat
     8941 aattttcttc gctgtcccaa atatggtgta aaatttgttc ctttaataat atttgacctt
     9001 tattttcaat tagataactt agaagcttat attctacaga gctaagttcc aaacattctt
     9061 tatttttata tacttttagt aacttttggt ccaattttat tccattggaa tatattactt
     9121 tattattcga tttttttctt attgctactt tgactcttgc ctttaataca gataaagaaa
     9181 atggttttgt aatataatca tcaagcccta tttctaatcc ttttacttca tcaatttctg
     9241 agtctcttgc agtaagcatt ataattggta tatctgactt ttcccttatt atttgacata
     9301 catcaaatcc atctccatct ggaagattgc agtctaagat tataaaatct atatttttac
     9361 tattaaatat atttaatcct tcttgttttg tataagcctt aaatacatta aatccatcat
     9421 tctcaaatgc ataaaaaaga ccttcattta agtttatatc atcttcaata attaataagt
     9481 tcaattttac tctcctttag attttttaga taaataatta ttttcttgca ttatcatact
     9541 aaaaatattt taattttatt tatttcacta aaaagattgt atttaaattt gttttcaaaa
     9601 taaaaacatt ataatcaagc atatagttat atatttccca atttaattac gcttaaattg
     9661 aaaatataac tttattctta tataattaaa ttatacaaca ataaagttat attttttaaa
     9721 tatattttta ctagggggaa tagaattgaa aagggttgat gagagaaata ctatgtttgc
     9781 aagagcaaat tacaaaaaag atagtactgc ttataatgac tattataaga aaaatcctga
     9841 caaaaaagaa attgatgata gtataagaaa tagaccaaat ttatgtagtg aaggaacaat
     9901 gacttacaat gagttaaact ctccaatggc aagtagtgca ttcgattttt tatctgatat
     9961 caaatcttta tgcgaaggaa aagtttctga tactaaagta gatgtagatg caaagtcaat
    10021 gacaaaaaaa attaagggtc ttgcaaagca gtatggagct tctgtagttg gtataactaa
    10081 attaaaagac tatcatattt atacaaatag gggtagacat gaagaaaatt acggtgaaga
    10141 aattaatcta actcataagt atgcaattgt atttggatgt gagatggata aagaaatgat
    10201 aaatagagct ccaatgatat gtgaagttat agaaacatca aagtgctatg tggatgcatc
    10261 tatcgtcggc atgattttaa gttactatat aagaaattta ggatatgatg ctagaaatca
    10321 tatggatgct aattatttag taatgcctgt ttttatagct cgtgatgctg gtttaggaga
    10381 tataggaaga aatgctatac ttacaaataa agattatggc tctagactta gattaggtgt
    10441 tgttactaca gatattcctc ttttagaaga tgaatatgtt gattttggac tagaagattt
    10501 ttgtaaggtc tgtaaaaaat gctcttttaa ctgcccttct cattcgcttt ctaatgatat
    10561 taaaattggt gatgatggaa aatataattg ggtaattgaa catgagacat gctatattaa
    10621 atggagatat ttaggtacag attgtggtat gtgtatatct gcttgcccat ttagccaaaa
    10681 tctcgaaact ataaagaata ctacttcatt taagggtaat aatgagctta ttcaaaaagc
    10741 tttagatgag tatacttcaa aatttggtaa aagaatattt atacctggaa atccatcttg
    10801 gttgaaataa ttttatttaa taaagaatct gtactagaat ttatcatagg acaggttctt
    10861 tattaaatat ataaattttt aaatactttt tctcgtttat aacaattcag caatgttttt
    10921 agtctataat tgacattata aaatattaaa ctaaaaggag taactgtatg gaaaaaatat
    10981 tagtagtaga agatgattct atattaaata aaactctatc atataaccta attgaagatg
    11041 gatatataat tacttcaaaa tttacagcta aatctgcact aaagtctatt tttgaatgtg
    11101 aatttgattt aataattttg gatattaatt taccagataa aagtgggttt gaattatgta
    11161 atgaaataaa ggaaaattac aatataccaa taattttttt aactgctaat gatatggaat
    11221 gtgatatgat taaaggctat gaacttggag ctttggatta tataacaaaa ccatttaata
    11281 tcaatatatt taaacaaaaa attaaagctt ttttaaatca tcttacaata aaaacaaaac
    11341 aagattacta taaggatgga tatcttgaaa ttaacttttc tgagttatct gccaatataa
    11401 atggaaatca aattatattt acaccattag aatatcgtac tttaaaactg cttactgaaa
    11461 atcctaaaag tattttaaca aggaaagtac ttttagaaaa actctgggat atagatgcta
    11521 actttgtaga tgaacatact ttaacatctg ttataagtag gattagaagc aaaattgaaa
    11581 aagacaatct taagtatatc aaaacggtat atggtatggg atatatgtgg ttaggtgaaa
    11641 gaaatgaatt ttaaaaaatt ttcttttagt tctatttatt taataattgc tgttggtata
    11701 atttttacag ctattttatt tagtatctca atttatctaa taactggtga tttaaaacta
    11761 attgctctat tattaatata tacaatggta ctttgtggat ttatagcatt aataatattt
    11821 ttacttcata aaaaaatagt tatattttgt tctaaaatat gtcacacttt agatagtatg
    11881 atgaatagtg aagataatat tgaaattgaa ttagaagaag aaactatttt atcacgtatc
    11941 aatcacagat taattagaat gtatgaagtt attcaagaaa atcgcaatag tattgctata
    12001 gagaaagctg atttacaaga actagtttct gatatatctc atcaaattaa aactcctatt
    12061 gcaaatttaa aaatgattaa tactactcta ctaaatcaaa catttaaaac agaaattcaa
    12121 aaggattttt taatggatat ggaaaaccaa ttagataaat tagatttttt aatgcaatct
    12181 atggtaaaaa cctccagact agagacaggt ataatcacac tatctaaaaa gaaaaattca
    12241 atttatgaaa caattgctac agcacttagt ggaattttgt ttgatgctga aaaaaagaat
    12301 attgaagtta cagttgattg tgacccaaat ttatatataa ttcatgacaa gaaatggact
    12361 tctgaagcta tttttaatat attagataat gctgtaaaat acacaagttc taatggtaag
    12421 atacgtgtag ctacagaatg ttgggttatg tatacaaaaa tagatattat tgataatgga
    12481 aaaggaatat cagaaagtcg ccaagcagaa atctttaaaa gattttatcg tgaagaagat
    12541 gtccatgaca tagaaggtat tggaataggt ctctatcttt caagaaaaat tatatctctt
    12601 caaggtggct atattaaagt tacatcagat attggaaaag ggtcgacttt ttctattttc
    12661 ttacctaata aataaatttt taaaaatttc atttgtaatg attctgcaac attttggttt
    12721 tatactgtat ttacaagatg ttaaggaggt attattatga gtattttaca agtaattaat
    12781 ttaaagaaat attatggaca aagttcaaac attacaaaag ctcttgatgg tgtaactcta
    12841 tctattgaaa aaggcgaatt tatttctatt gtaggtacat ctggtagtgg caaatccaca
    12901 ttattaaata tgatgggagg attagataat cctacatctg gaaaggttat tataaagggt
    12961 aaggaattat ctactatgtc tgatgaacag cttacaatct ttagacgcag aaatataggt
    13021 tttatatttc aaaattataa tcttgttcct ttacttaatg tatatgaaaa tattatctta
    13081 ccaataggat tggatggaga gaccatagat aaggatttta taaatgaaat tataaatatg
    13141 ctttctcttg aagaaaaatt atatgatata ccaagtaatc tttctggtgg tcaacagcaa
    13201 cgtgttgcta tagctagagc acttgtaaca aaaccagcta ttatactagc tgatgaacca
    13261 acaggtaacc ttgatagtag aactagttca gatgtactga gtttacttaa agtgacaagt
    13321 aaaaaattca accaaacaat tgtaatgatt acacacaata atgaaattgc acaactaact
    13381 gatagaatta ttcgtattga agatggaaaa attgttaact aggaggtgta gtatatgttt
    13441 agaaataata atcgcacatt tataagaaaa atagctttaa atgatttaaa aattaataaa
    13501 cttaaaacat atctttcagg tatcattata atgatttcta catgtttatt attaacagtg
    13561 accttagttt catataatgc ctctgtagat ttagttaatg catctccata tcatgctata
    13621 tataaatctg tagatgaaaa agcaaaaaat atactatata aaaataaaaa ctttagtggc
    13681 gtaggaactt acaaattaat tggaagtaat aaaaagacag attgtattat gtcgattgtt
    13741 tatgcagatg atacagcaat taaacttatg aattttcaac cattgaaagg caaactacct
    13801 acaaataaaa atgaaattgc tatttcagaa aaatatttac aggaatttgg tttagataaa
    13861 gatattggag atagcattaa attaaattat tatgatgata ttacgaataa agaagttcaa
    13921 tgtgatttta ttattgttgg atttctagaa aattattatc aagataatgc caagcaatac
    13981 tatacagtcg tttcaaatga ttattttaag aatatagctt ctacaccttt acaaaataaa
    14041 agtaatactt ttgacgagtc aagacctgat actgtagatg tattagttaa actatcagaa
    14101 gaaaatacag agaaagattc agcttcaatt aagacacagt taaataaaat tggtttatct
    14161 ttaggtatta agaaatataa tatatactta aatgataact atattgaatc aaatttaatt
    14221 gatggtgagc agattattac agttttagta gttggaatag ttgttctttt ttctagcgtt
    14281 tttgttatat atagtatttt ttatatttca gttgttaatt cagttcagat gtatgctaag
    14341 ttgaaatcgt taggtatgac ttcttttcag ttaaaaaaaa ttatttctct acaaggaaat
    14401 attttgtcaa ttatttttat tccattaggg gttatagctt cttgtataat tgcttacata
    14461 attcaaccat tagcatggca aatgatagct gatttattta taattttaat tttatcactg
    14521 gtaatgtttt taactgttag aatttcttta tttaaaccag ctagaattat ttctaaaata
    14581 tctgctattg aagccatgca atatacagaa actaaattta gaaaaaaagc aaaaaatttt
    14641 agttatataa atataaaaaa tctagcatta aagaatatcg agtcaaatag aaagaaaaat
    14701 ttgattgcat taatatcgct aagcattagt ggagttttat ttatttcaat cgctaatctt
    14761 gctaattcaa tagatttcaa aaaacaacta tctcaacaat ttgtctataa cgaagattat
    14821 ataattagca ttaagagtga taacttatat gagcatatta cagaaatcca acaaaataat
    14881 ccactaacag attctcttaa gaatgaaatt aaatctattc atggagtaaa aaaattaatt
    14941 gaatccaaat caattaaaag taatattata gaaccttaca tcaaagatag agacgaaaaa
    15001 caatttatta ctctaattaa aggtattacg cctgaacttt caaaatcact tgaagaatat
    15061 attgtcagtg gaaaaataga ctatacacat ttagattcag atagtttaat tataaataaa
    15121 tatagaacaa aatattatgg attaaattta aaagttggag atacagttac atttaatatc
    15181 tttagtggaa atgatattat taaaaagaaa atgaaaatta taggaatcat tgacaattcc
    15241 aatacaggag gtatgttttt tgcttcagac aaaactttag aatctctaac accatataat
    15301 acaaatttag atttttctat tttaatagat aaaaagcatt ctaaagaagt tgaagaaaag
    15361 ttgttaagta taattcagaa aaattcaaat ttaatgcttt attcatatga agatgattat
    15421 agaatgatta ctagagcatt tcaatatata attatagctt catacatttt tgttggttta
    15481 atttcttgct ttggtatact aaatatgacc aataccctaa taaacagcgt tttaattaga
    15541 aaaaaagagt ttgcattact tcaagccgtt ggaatgacaa gaaagcaact gcgaaatatg
    15601 ctttatagag aaggtctaaa cataagtatc aaagctatat gtacttcaag tacactaggt
    15661 tattttggtt caaatctact ttgcactttt ataaaagatg taattagatt agactttatt
    15721 aattttaaat tttctatatt tactatttta attttttcat tcgtattaat tggtatacaa
    15781 gttttagtga ctgaattact tgtaagaaat atagaaaaaa aatcacttac agaacgctta
    15841 cgttctcagt aagaatgtat ataaaaaata gccatttttt atacaagttt taatatgatt
    15901 ttttaaaagc tatagggata gaaaattttg actttacata attttctatc cctatacttg
    15961 tttttatttt atacagatac acttacttat atctccgagc aacatcacca gagacataaa
    16021 caagcatatt ccttattttc taaatatgtc aattgcatga cttgtaaccc ctactaaatc
    16081 ttcacgttca caagtagcaa ggtgatattt taaatataat gcaaatgcat catcatccat
    16141 agagtctact gcttcacgca tatataaagc caaaccatct gatgcgacat aatgcagtct
    16201 agttacatta aatgcagaca tcaaatcatc aatgttttct ttacgtacaa gctcaaacaa
    16261 atcctttggt tcagacttag cagcaaatgt ctgtggatca atcaaacctt tttcaatata
    16321 ttcagataca ttaatatttc ctctatggaa cccctcgtca atgagacagc catcagatat
    16381 aacatatgca gcaaaaacca caccaccagg ttttgttaca cgaattgcct catgtaaagc
    16441 ttgttgttta tcttctttgt tataaagatg atatagtggt cctaacagta acgtaatatc
    16501 atatttatta tctgggaaaa cagataaatc catagcatta ccctgagtaa tgcttatatt
    16561 ttcatttgat tgagtatttt tatggaaaac ctctatatta tgttcgacta attccactgc
    16621 gtcaacatca tatccctgac gtgccagtgt gtgagaataa cgaccagttg cagcgccaat
    16681 ttcaagcaca caatcacctg attttatgta cttatcaatg taatgcatag tagtgagaaa
    16741 ttccaccatt ccatgtttta atgcaagtcg actatcttca tcataagtat tataaaagtc
    16801 aattaagtat tgttttgttt ccataatttc acctcttcta tattagttaa taaacatcaa
    16861 atagacaaat tccaatctgg tttttcttgt cttataaaag ctgttaatat atttttcaac
    16921 attttttcag cactatatct atctaatgtt aatttttttg ttgctgttaa gacagctatg
    16981 ccatgagtat aaagaaataa atctaaaaat attacttttg cacattccaa ctctattcca
    17041 atgctttccg aaaaaagtct tgcctttttt tcattgtcaa tttctttata aaaatcattt
    17101 gcctcttcca tcatcaaatc taaatcattt ataaataaca acttaaataa atgagtttct
    17161 tcttgagcaa attcaatgta tgaaagcgga agcagtaaat aaggacttac acttttagaa
    17221 ttactataat taacaacata ctgattatag tattcatatg caaaagcaag aaattcattt
    17281 tttaattcat tcatgttttc gtaacaagta aaaattggtc gagtggaaca ctgtaattta
    17341 cttgcaatac tcctcgcatt tacagcttca aatcccactt ctcttgtaat atccaagact
    17401 gtattcaaaa tcatttcttt tgttatcttt actttaggtg gcatatatgt tcacctctcc
    17461 attaaatttt tataaaacac atatgttata taactatagt aacatatcaa gaaattatgt
    17521 caagaaaaag ctttgcattt ttttgcaatt cctaaaactt ataaataaaa catgagttgc
    17581 aattaatcta ttaatagata acagcaactc agattttcct ttaaatactc ttctttttgt
    17641 ctaatatttc atatacacaa tcttctattg aattaatatc tttttctttt ctttttttag
    17701 aatcaattaa aatgaaatac agtataaaac ctacagcaag tccccatgtt ccgcctttag
    17761 cagcaattac acctcccata agtccaatca atcccttctc aacacctgta ttacatagct
    17821 ccattgatag ctttgtacat aaatatcctt gaactaaaag tgttactgac aatgctaatg
    17881 gtataagagg tgtagcaatc tgcgcaattg gataaaaaca gataaaaatt gctgaaaccc
    17941 atactaatgt acccatgcca ctaaaaacag actgcatggc ttcctttcca accttgtacc
    18001 tttgagcaac acttcctgta agtgtcgcag ctaatggacc gcacattggt atatatggag
    18061 aaaagattgc cataaaaata tttcttatcc cacttataac attagaccta tttgaattga
    18121 aatctatata ttcatctttt ctattttcac tttcagaaag tactaagttt tcacctgtta
    18181 taaaatcacc aaaggcaatt atgtatatca taaaagccat tggtatccct tgtataaata
    18241 acattacact tggaaaacct attccaaaag gagatactgt atttatcaga tttttaaaat
    18301 ctactatgta aactaaattg tcaaaactaa atctaggaat ttctatttct ttactaaata
    18361 tacctactat taaactcact aaaatagctg atactacacc atattctcct aaatgaaata
    18421 aaaatttatt cttaactttt agagtatcaa atctttcaga aaacattaca aaacaagcaa
    18481 ccaaaattcc tactgttata gatataggat aagaatcaaa tctcccttta ggattaaact
    18541 ctgatattat agtcgatata ccgacaccca ttagtattcc acctttaata gaatttggta
    18601 tcttactaac taactttcca gctacaccag ttatacccat taaaataaat attattccta
    18661 aatctatttg taaagctgtt aatgcttgta ttctttcaat gcccatgctg aactttaaca
    18721 aatatgcagt tgtaagagtt atagaagctg taatccagcc aggtacaact gggtctccca
    18781 taaaaacatg caaacagtat aatattgagt taatgatagc catactccat gccacttcat
    18841 agggtaatcc caaaatttgt gtactaaggc caattgtgcc taaacttgtc atacttgata
    18901 ttataccttg aatagcttct ggtttctcaa atctgtaatg tataaatgga attcttatct
    18961 taaacattcc aaattttata tgaggttgtt cccttctagc gagtctttcc ataacttcac
    19021 tccacctttc aataactctt ataagtttac aactttataa ttgtttataa attatcaatg
    19081 tcattaaaat tagtatataa catgcaaatt atataaacaa taaattaaat ctataaaatt
    19141 gatagatttt gtgcatatat agaaagaccg cttcattttt agttacttaa atcacttaat
    19201 attagttatt taaatcactt gataaaggtt ttttataaaa ttcaatagtt ctattccatg
    19261 attcaaactg tccttttgaa tttccttcta cagttccacc tgaagaaaaa atccctctac
    19321 tcatactcat acataaagtc tcctttagag gcataatata tggaattggt aacggataac
    19381 ccatattatt gtatgttaat aattctacgt tatatggata attattttct tcaaagattt
    19441 ctaatgcctc taaacaagct tcataactat tccatatgtt atcttctttt ccagcaatca
    19501 taagtatatc tgctttggaa ttttctattt ttattctagc ctcttttcta ttctctgctc
    19561 ttttaagact tttcttataa gtactattaa atccaaatgg aatatctttt gatatatttt
    19621 ttttctgttc ttcaaagaat atatcattgt ctactttgat atatggaaat ggctctcctt
    19681 tatatgacca tgatgaagta tcatttccac tcatcaatcc attaagagct tggaaacaat
    19741 aaatgtgagg ttgtgatact acaacctttt taatttgttc atatcttgaa gcaagtaaaa
    19801 aggccaattc tccacccttt gatgtcccat gtacaaatat ttctttagta ctggtaattt
    19861 cattttcact aatccactca aatacttttt caaaatattc taatggaacc tcttctaatt
    19921 tatctggtaa cccttctaaa gcaaagtatg caactgttag tacattgaat cctctagatg
    19981 caagtggagc tgctaaaagg tctagagcgt ctaaatttcc atcagaacca cctagcatta
    20041 aaattgtttt attattagaa ttttgtttat aaaataattt tcctataaac tcatcagata
    20101 tgtattttgt ggtaatatct tctgatttaa atattctctt taattcaact tcttttcttt
    20161 caatagaagt ttcaaatatc atattcataa ttattggttt atcaatagat atattttctg
    20221 caaaattttt accttcagtt ttagattttt ccagcgaata aattaacccc atacaattat
    20281 cagccttata agtaccttct ataggttcaa ctaaatctaa atctacttct cctttttcat
    20341 ttgaacaaaa cactccataa gatgaaaatt cttctccctt acaccatgga aattccattt
    20401 tcacacttat tttaagttta gtatttggaa ttaagtctga aaccataatc ttaagtcttt
    20461 catccacaaa tgattctaca ttgtatacat ttactttcat aatctatttc tccttataat
    20521 aattatttat gttttatata aaacagcttt atctaataca agataaataa atttttaaat
    20581 taagacacca tattgactat ttcattttat caacatattt catctttgtc aacatattgt
    20641 cttttttagt aatttaaaat tatggtctat atgaaatcca gttgattttt ggctaattaa
    20701 agataggaat gctattaggt ttagaatata tatattcaaa ataaaaaacc actcatttaa
    20761 agaatggttt ttaatataaa ctatttgtta ttaataagga tattttttta tttaaattta
    20821 tataatcgaa catctaatat ttaatgttta ataaaattat gcatttgcaa aagtatatat
    20881 ggcattaact cttgctacat ataaattctc atcactacat ccaatatatc tagcatattt
    20941 ttcaatcttt tcttttgcat tttcaacatc attttcatcg ctctccatta actcatttat
    21001 ataaatatcc attaatgacg ctttatattc tcttatatca tagcctaaaa attcagtcaa
    21061 tctctcaatt tccttactaa aatctccatc ttcttttacc aaaaattcgt atacatcttc
    21121 cctatatgga tttaaagtta ttatttgttt tactactttg cattcgtcag ccttcccaat
    21181 aaaaccatct ttataattat taaataaggc attagatttt tttatagatt cttcatcaat
    21241 ataagaatgt accgcagtta tacgattttc tataagggca tctacaacag cataatgaat
    21301 tttaaaacaa tcattgtata aataattgct cattttcttt attgtcgatt ctatttctaa
    21361 gaagctcgcg tggccatttt ttttattttt atttggtata ataaaagttt tattcaaatt
    21421 attaaaaagt ctcttaaaat ctaaatattt ttcacaataa acacttttaa aggtatcgat
    21481 atctatagtt ataattccaa aacttactat agtctcaatt cctttcttaa aagactcttc
    21541 tatatatttt tctcctaaac ttaaacctct atcagatatt tgctttagat tttgaaaact
    21601 atttaaacaa tctctttcaa agtttttaga tatttctaaa gcatataatt taaagatttt
    21661 ccttatctta ttataattat tttttgtatc atcaaatctg ataatagctc catttacatc
    21721 aaaagtaatc attgagtttt aacctcccca ttagtttcat caataaaata aatttaatat
    21781 aaactttata atcataatat taaacttatt tatactataa ttattaattt gatgataaaa
    21841 gctattttcc ttctaaataa aaaatttgtc taaagtttct aaaaaattta gacaaatctt
    21901 aggttattac tctttaaatt atttttaaat tattccaaat acgccaaata atattggtat
    21961 aaatatagct ggtaacaaat tggctacttt tatattcgtt aatttaagta catttagacc
    22021 aagtcctata attaataaac ttccagctgc tgtcatttct gtgactacag gatcacttaa
    22081 aaatccattt aaaaatgatg ctcccacaca tataatacct tcatacaaaa atacagatat
    22141 tgcagaaaac attacaccta tacctaaaga agcagtaaat ataattgaag ctattccatc
    22201 tataacagat tttacaaata aagtatcatg acttcctgta agaccacttt ctaatgaacc
    22261 aactactgcc atggctccta tacagaataa taaactagat gatataaaac cctctgctat
    22321 agaatctttt ttatttcctt tagaaaattt actttgtaaa aaagcaccca acttatttac
    22381 ccatttgtca atatctatta attctcctat cagagcacct acagctacgg atattattgt
    22441 cactaatgtg ttttttcctt ctagtgctcc tgatatacca atgtacaata cacataaagc
    22501 aattccattc attatagtat cgcttactct ttgaggtaat cctcctttta ttataagacc
    22561 tactatacat ccacctgcaa ttgctagact atttgctatt actccaaaca ttttttctcc
    22621 cctcgattag ctaattgaaa tttatcctat tattaaaacc taaacatcta tactttgtat
    22681 tggatatttt aaatattcca aaaattcttc aaaacctttt ctagatatat ttacagtttt
    22741 agtatttatg ttaggatgaa aatttaagta atcttcttta attacatctt tatctatgta
    22801 aagctcaact tttctttctg tatcatttat taatgaaaaa ggatttacac ttccaggaag
    22861 aagtttaagt acattgtata gtctttcagg tgaagcaaac gataatctag aacttcctat
    22921 cttatcttta agtgagttta aatcaacttg tttttctcct ttaactacaa taagataata
    22981 cttatttcct ttggcatttc ttaaaaataa atttttacat tgacacccct ttgttattcc
    23041 atctaaaatg actaattgct ctgctgtata tacagcttca tgctctacta catcatattc
    23101 aacatttaat ttatccaaaa tatcatatat tttagtttca ttcatgctag attcattttc
    23161 atcttgttct tttttattat ttaaatcact catacttttc tccttaaaca cttcatcata
    23221 atatcattgt gaggtattat tctactatag ctattgctct tactggagct ccatcagcat
    23281 cattatactt aaatggtgct gctataaata gaaatttttc tggaacattt tctaaatttg
    23341 taagattttc tatgattatt tttccttttt caaataaaat atgatgcact tcaaatactg
    23401 atgtgtcata tctatccact gatagcatat ctattcctat gccttttata ttagtattag
    23461 ctatatagtt tgcagcttct tttgttaatg taggataacc tacatagtac tgctttttat
    23521 cccaaaattt tgaccaacct gatttaaata ttataaaatc actatttttt atttcatctt
    23581 catatttttt tagatattct aattctatat tctcatttcc cttttcaagt tccaatacat
    23641 aagcttttcc aacaaaattt tctatatcca acttatctag ggtctcacct ttagtataca
    23701 tatgtcttgg tgaatccata tgagttccat tgtgagaaaa tacactaatt aatgtttctt
    23761 gataaccatt ttcttcaata atagctactt tcttgatatc tggtctatta ggctctgcat
    23821 atacaggcat gtcattatgt gtcacatgag ttaaatcaaa aaccttcatc tttatatacc
    23881 ccctatgttt ttattttcta ttatatacct aaattcattt tatttctatc agattttatt
    23941 ccattatata ccatactact atatattttt tataaacctc aagggattta ccgaaaaatc
    24001 ttggaaagaa ttatcagtaa atataacatc ttcacccaaa aaacgtttat accttccttc
    24061 ttctttcttt aaagtaattc tctgtttttg tgaatgatta ttaaaaacca ttatcttaag
    24121 aggaggtttt tttctattag tctgcactct ttttaacatg tattttatgt gcatatcagc
    24181 ttcaggaaat gaatatccac aaaatattaa aaggtcagta tctcttaaag ttttttctgt
    24241 ttcattccaa acattactta aaaatatgtt tgacatattc ttaaaatatg ttggtggcac
    24301 tataataggt tctgttaact caccacactc taaacatttt gtctctgaac tattttcaat
    24361 aagcttcatg acaccacctt catgtggtgt aagagttaca ctattacaaa tggggcaata
    24421 aagccaattc aatgacccat gtatcttata taatttgatt aatggttctt ttggttttct
    24481 ccaatttaac tcctctttaa aatttgtaaa ctccacacca taatcaagca taattggcaa
    24541 atcattctta tctcttaatc cagtaactgt attgtctata tgtatatcat aatttgcact
    24601 aataaaagta gtatcaagaa gcaaatcatt ttccaaaaga ttttcaacta aaagtttatg
    24661 atatttatta tctgttctag gtgtattatg aattgcttca gccattagca gtattaaata
    24721 ttgtcttaaa aaacgtatac tatcacttct gttatttaga ttttctagtc caaaatttct
    24781 aaatgcttct tttctttgtt ctgctaagtc taaaagacct aaaacttctt caaaggtagg
    24841 aaaatttgca ttatctatat tatcttttac tatatcaata ttgaacatct gtttaaagaa
    24901 aatatacaat tctctattca ttttggtatt ataatttgtg ggatttagtt ttttaaaata
    24961 ttcgctaaaa agttcatttt gaataggtag attctctgct gctgaagctc ctgctcctaa
    25021 aaatattgct attttcatat agttcctcct aatataaata ttactcctat ttttatatcc
    25081 attaaatatc catatcattt attttatgat acaacaataa agctgtcttt tactagaaaa
    25141 aatcctatta caagacagcc ttatattata ttaaatcttt aataattcgt aaatagaaag
    25201 agcatatatt tgagtagctt ttataatatc ctctactatt aagtattcat tagcttggtg
    25261 ttctgtttct ggttttcctg gaaaaatagc tccaaatgca acacaattat ctaaagctct
    25321 tgcgtatgtt gctcctccag atgaaagtgg tgttccatca agaccagttt cttcttcata
    25381 tacttttcta agtgtcttaa caagaagagt atcttctgga acatagattg aatccaagaa
    25441 atcatattct tcataattta aattgtattt atctgtcatt ttctttaact cttttacaaa
    25501 atcatccttt ttgtatgtta ctggaatcct tacatctatt cctgcaaatt ctttttcgtt
    25561 atcaatagtt actctaccta tatttactgt tagtttacct gatacatcat ccttgcaatt
    25621 aggaattata ttgtttccat tagcatcttc accaataact tctgctaaaa acttaattat
    25681 attagaatct atacctatat tattaagagc tatacaaagt ctagccactg catttatacc
    25741 agtatcactt gctgctgaat ggacactttt tccaattata cagattttat taccttcaac
    25801 agtatactca aaatttaatt tatcaagttc tttttttaat ttatcagagt attttccaat
    25861 atagattgct ttacctggaa ctgcatttaa agcctttcca actgacaact ctatatcact
    25921 tttgctatca caagtcaaat gaacttgtaa aagacctttc tctgcatttg ttattgggaa
    25981 tctagaatct ggagtaaatc catagtttgg tatttcttca ttattttctt tatatttatt
    26041 tatacatctc caaagatttt cttcatcagc cccaaaaatg aatcttatcc tcttattaaa
    26101 gtctacattt aaatccataa gagctttcac tgcataaatt gctgatattg taggtccttt
    26161 atcatcttgt gttcctcttc catacaattt accatcttct aaaacagctt caaatggagg
    26221 gtaattccag ctttctaaat caccttctgg aaccacatca acgtgaccaa gtatacctat
    26281 cattttttca ccttgtccta tttctgcata tccataatat ccatctttgt atactgtctt
    26341 aaatcctaaa gatttacata agtctaatat acctcttaaa gctttatcta cattttcacc
    26401 aaatgggcaa ttttcagttg cttcagatat aacactaggt atttttacac tttcttgtat
    26461 tgaagaaatc atttcatctt gtagagaatt tactttttct ttaatttgct ctttcatatt
    26521 atcacccttc aattttcatc ataatatact aataaatatt ttgacctttt aaaaaaaaaa
    26581 tatgcttatt ttttcagttt ttttataaat tcaatcgctt ttaaagtccc catttttgaa
    26641 tggtcaaatg taagtccacc ttgaaaataa acattataag gaggtcttat tggagcatct
    26701 gcacttaatt ctatagaaga accttgtata aatgctcctg ctgccattat gacttcactt
    26761 tcgtatccag gcatgtccca aggaacaggt tttacataag aatcgactgg tgcagcttct
    26821 tgtatacctt cacaaaaact tattacttca tcagcattat taagtctaat acattgaata
    26881 atatcacttc ttaagtcatc atattttggt aatacatcat atcctaattt ttcaaaagct
    26941 cttgagcaaa agatagctcc cattacagcc tgagatacta tataaggcgc caagaaaaaa
    27001 ccctgaagta catttctagt tgttccaaaa gtaagaccac attcttttcc tattccagga
    27061 gaagtcattc tataagatat aagctcaatc aaatcttttc taccagctat atatccacct
    27121 gtcaatgcaa gcccaccacc aggatttttt attaaagagc cagccataac atctgctcca
    27181 acatcagtag gctctttggt atctaaaaac tcaccataac aattatcaac cattactata
    27241 gcttctggtt taacagactt tattacttct atagcttctt ttatatcact aattgaaagt
    27301 gattttctcc aagaatatcc ttttgacctt tgtatcataa cgagtttagt tctatcattt
    27361 attttgtttt taattccttc taaatctaag tttccatcct ctaaaaaatc tacatcatca
    27421 tatgtaacac catactcttt caatgaacct ttttcttctc ttatgcctat aactccctct
    27481 aaagtatcat atggtctacc agtaacagat aaaatttcat ctcctggtct aactatacct
    27541 tgtatacata aagtaagtgc atgtgtacca tttacaatta ttggtcttac aagagcatct
    27601 tctgtattaa atactttaga ataaatttcc tctatttttt cacgaccaat atcattatat
    27661 ccatatcctg tagtccagtt aaagtgcata tcacttagtt tagattcttg catagctttt
    27721 aaaactttat attgattata ttctcttatt tcttttattt cttcaaactt atcttttatt
    27781 tcttccatta tttcttgcga tagtttaaaa gtatcatcat ctattccata gtagtctttt
    27841 aataattctt ttgtctcatt aagcatttta tcacctgtct ctttattatt ttatttaaac
    27901 atgtaaagga gactatttta ctaaaacaaa taaattatag tttataagtt agtataagtc
    27961 tcctctcaat catcttaaat taaattaatt atctatctgt tgttattatt attgttgttt
    28021 tggttttgat ttgttagatt aatatacttt ccaggttgta tagttgatac agcatgttta
    28081 taaatcatgt tttgttgtct attatcccct tctattaata ttatataact atcaaacccc
    28141 tttacaagcc ctttaacttg cactccatta actaaatata tagtaacagg tatcctttct
    28201 ttccttgcat tatttaaaaa caaatcttgt aaatttaaaa ctgtattttt cattagattt
    28261 gtaccctccc aagattaaat tgaacttcta aaatattcct accttatatt ctatataagt
    28321 tatgacaaat tttcaatatt aagttgtata cttaaaaatt tatatattat tttatagaat
    28381 ctttaatata taagattatt tcatttttta attcgtctat attttcatat tggtctaaat
    28441 caaaccattt tgctgtttta tatcttttaa accatgttat ctgacgtttt gcataccttc
    28501 tagaatctct tttaattatt tcaatagctt cttcgtatgt atattctcca tccagatact
    28561 ttaatatctc tttataacct ataccttgca ttgatatcat atctttttta aatcccatac
    28621 ttaaaagttt ttttgcctct tcaaccaatc catttttaat cataatatca actctcatat
    28681 ttattctttg atacaaatgt tctctatctc tatttagaac tattattatt ggttgatatt
    28741 cttcattaaa ttttaaatca cttgaaaaat cattcatttt cttaccactt agacaaactt
    28801 caagagctct tatgaccctt ttagtattgt ttttatggat tctattagca gattcttcat
    28861 caagttcttt taacttatta tgcatgtaat ctattccctt ttcagcaagc tgtttttgaa
    28921 gttcttctct taattcatta ttggcatcag attgtgcaaa atccatatta tatattaaag
    28981 agtttaaata aagtcctgtg cctccagtta ctaaaacaca tttttccttt tcatttattt
    29041 catgtatata tttttttgct ctttgttgaa actctgaaac tgaaaatgga tagtcaggct
    29101 taacttcatc tataaggtaa tgcttcactc cttgcatctc ttcctctgta acttttgcac
    29161 ttccaatatc catatattca tatatttgca tagaatcagc tgaaattatc tctgcattca
    29221 tatcttttgc taattttatt gacaaatctg tttttccaac agcagttgga cctgtaagta
    29281 ttataagtgg tatttttttc ataacattcc tttccatata ttttaaatat ccaaatattt
    29341 tacataattc ttttaaacat tttttctatt tctgtcttag atatttcgac cattataggt
    29401 cttccatgag gacatgtaaa tggattctca cacttttcta gctgttccaa taaacttttt
    29461 atttcaatat cgtatatttt atcatttgct tttatagcag acctacatgc catagatgca
    29521 aatctctctc cttttaaatc ataattactt gtaatttctt ctatattatc aattatttga
    29581 agtataaatt tctcagtttc aggtactcca aatatagtag gaacacatct taccattata
    29641 tgattattgc caaaaatttc tagctcaaaa ccaaatttca taaataactc caagttattt
    29701 tcaatttgaa gcatatctat atttgatact tcaataacca ctgggtctaa aagtatttgc
    29761 atatttatat cttgtctata gaacttctcc atatatcttt catatagtat tctttcatgt
    29821 gcagcatgtt ggtccaacaa atacatagaa tcgtctttgc tcaaaattat ataagtatta
    29881 aatacaactc caattacaaa atatccgtat aaagaaaact tgcgttttga actacacaag
    29941 tattcttctt cactgtcctt aatactatct cctaagtaat aatttccttc atttttaatt
    30001 ccatcaactt gaaactcttc ttgtatatta tcatttgcac tatcttcact tagataatta
    30061 atgtttttta catcatcttc tacacttgca tttaggactt cacttactga ttgaaattca
    30121 tttattggtt tttcacttga taaagtaaca acttcaatga cttcatcctt acctttagta
    30181 atattactat ttttaggagt agattctaat aaatcattat ttcttaattt ataatctgta
    30241 cttttttctc tactgttaat agcgattcta ggttgtgttt ttttatcact ataagtagca
    30301 tattttccaa taagatttga gtgtattaat ttaaccttta aaaaatctct aagttctata
    30361 tacacttctt gttctttttc aaatttaatt tcaagtttat tggggtgtat atttacatct
    30421 atacatgatg ggtctacttc tatatttaag aagcacactg catgtttacc tattggaatt
    30481 atagacttat aactttctgt tattgcatct atgattattt tactttttac aaatctttta
    30541 tttatataga tatgttgtaa gtttttattt gaacggtata tattgttatt acctatatat
    30601 ccattcattt taaagtgatt gcacttaaac tctacatcta ttatattttc cgttatttct
    30661 tttccatata tacttctaat agtgtttaca agttttccat cccctggagt atttagcatc
    30721 tgtttattgt tatttgtata tttaaattga atgtttggat tacctattgc aagtttattt
    30781 atcaaatcac ttatgtttat agtttctgca tgagtagatt ttaaaaattt ctgtcttgct
    30841 ggtgtattaa agaaaatatc ttttataatg atggtagttc cattagtaga acctattggt
    30901 tctttcgata ttatttttcc accttcaaca tatattttag ttcctattat ttcatcttta
    30961 gttttagtcg tcatttctaa ttttgataca gctgaaatag atgccaatgc ttctcctcta
    31021 aaaccaagag agtataaatc atacaaatca tcaatttttt taattttact agtagcatgt
    31081 ctcaaaaatg atttttcaac ttcacttgaa ggtattccta ttccattatc tgtaatctta
    31141 attagacttt ttcctccatc aattatatct atggaaattt tatttgcacc tgcatcaatg
    31201 gagttttcaa taagttcttt tactacagaa gaaggtcttt ctacaacttc acctgctgct
    31261 atcttattta tagtcaaatc atctaggatg ttaattatat ttttcatttt tacacctcta
    31321 agattttatt tcttttgctt ttctttgaag attataaaga gagtttatag catctaatgg
    31381 agtcatattt aaaatatcca gatttaaaat ttcttctgat agtatatctc tatttacact
    31441 atcaaaagag atttgagtta aggctacttc ttttacagaa tcttcttgat taattttctc
    31501 tttatcatca ttttgcttca ttaaagcaac ttcatcattt aatgctttaa atttagaatt
    31561 taatttatca taatcctttt ttatatgttt gtagtctttt actataagtt catgttctat
    31621 ttttagagat tcatatttat tcttaaaatt actttgattt accttaacta attcttcgtc
    31681 caaattacta ttaattatat tattgttttc tttatctcca tttattgcaa cactgttgta
    31741 aacatgattt ttttccaagt ctttaagtat atattttgct ctttctatga cttcgtctgg
    31801 taatttagct aatttagcaa catatattcc ataactcttg tctgctccct gaggaattat
    31861 ttttcttaaa aatattattc cttccccatc ttctttgact gctattgaat aatttttaac
    31921 ctctttaaac tcttcttcta aatcagtaag ttcatgataa tgtgttgcaa ataatgtctt
    31981 acatctaata tttttttgaa tatattccac tattgaccat gctaaactta taccatcata
    32041 ggtactagtt ccacgaccta tttcatctaa tataactaaa cttctctctg ttgcattttt
    32101 taatataagg gatacttcgt tcatctctac cataaaagta gattgacctt gagataaatc
    32161 gtcactagct ccaactcttg taaatattct atctaatatt ggaatatcag cagactctgc
    32221 tggtacaaat gagcctatat gtgccataag agctattata gcagtttgcc tcatatatgt
    32281 tgatttacct gacatattag gaccagttat tatgttaata atgttttctc cacgatttaa
    32341 ataagtatca tttggaacga aattttcctc tcctactata ttttcgacaa caggatgtct
    32401 accatttctt atatcaagtt tattattttc atttattgct ggttttacgt agttatttat
    32461 atgtgcaact gttgcaaggg acacaaaaac atctatatta gctatagtct tagccacttt
    32521 ttgtattcta tctatgtttt tatatattgt atctcttatc tctacaaata tctcgtattc
    32581 taaagattta atcttttcct ctgcatgtag tatcttttct tctatttcct ttaattctgg
    32641 agttatatat ctttctgcat tactaagtgt ttgctttctt atatatgtct catctagctt
    32701 tgcctgttta aagttggctt ttgttatttc tatataatac ccaaaaactt tattaaatcc
    32761 tatttttaat gatttaactc ctgttttttc tctttccctg ttttctattt cctttacaag
    32821 aaaggctcca ttcttagaaa tctctcttag ttccttaagt tcatcactaa aatctgattt
    32881 aattatgttt ccatctttta tggttatcgt aggttcttct agaatagctt catctattaa
    32941 attgtatata tcatctaatt tatccatttc tgaaacatac tcttttagga tttttgcatt
    33001 tgataaattt atagtatctt ttaaattagg taacttttct atcgaatttt ttaagtgaat
    33061 taactctttt ggtgttactc tctcaaatgc tatctttcca catattcttt ctatatcgta
    33121 tatattttta agtatatcat ttaggtcttc tcttaatata aagtcatctt ttatttcttc
    33181 tattacatct aatctatttt caattttaga tttatttatc aaaggttctt ctacatattt
    33241 tcttaaaagt ctacctccca ttgctgtaga agttttgtca agtacatgta ataatgaacc
    33301 ttttttctta ttccctctta tagtttgagt aagttctaaa ttagttctag taaacatatc
    33361 tagaaccata tattctgatg aattgtatat atttatatta ttaatgttag atgttatttg
    33421 tttttgagta ttgtatatat aattcagaag aattgataag cttgacttta ttagaccttt
    33481 atcatcaaac tttagttttt gtagataaac atctggaaaa tattctttta atatatttat
    33541 atccaaatag ttatcactaa aactttcatt tatgtatata ttacttacag tagctatatc
    33601 tctaagcttt tctataaagt ctaaatcatt tataattatt tcagttggat ggatttttgc
    33661 aatttcttca attaccttat cttcatttaa acatgttgca ttagtttctc ctgtacttat
    33721 atcaacataa gttagaccta tattagtacc atccttatac aaagataata aataattatt
    33781 ctttttattt tcaagtaaat ttccatctaa aactgtacca ggagtgatga cccttataac
    33841 ctctcttcta acaattcctt ttgctgtaga tgggtcttcc atttgttcac ctattgctac
    33901 tttatatcca ttttcaacta attttgatat atatgaattg gctgagtgga aaggaacacc
    33961 acacatagga gcacgttctt ctaaaccaca agcttttcct gtcaatgcta tttcaagagc
    34021 ttttgaagct actaatgcat cttcaaaaaa catttcataa aaatctccaa gtctaaaaaa
    34081 cagtatacag tctttatatc tattttttac ttcaagatac tgtttcatca taggagttaa
    34141 tttattcata tctatttgca ttatcaaacc tccttctttt ctaattaaat attatatcat
    34201 agtagatgat tattattaac acaatatata aaattttgac atataactaa aaacaatcca
    34261 aaatgtatac tcacattttg gattgctaag tttaatgctt attaaataaa aatatttttt
    34321 tttacaataa agatattatt tcttttttaa gtttaatgat attttcaagg ttatcttcat
    34381 ttaactttct actatctata acaaattctt tctttactat tgatggtttt tttgaaataa
    34441 cataaattct gtttgatagc attatagctt cgtcaatatc atgagttata agaagtattg
    34501 ttgaatttac ttttttctta acatctaaaa gccatttttg catggatgtt ttagttaatg
    34561 aatcaagtgc cccaaaaggc tcatctaaaa gcattatgtc attagaattt ataaaagttc
    34621 tcaagaagtt agctctctgt ctcattcctc cagaaagttc tgaaggatat ttatcttcat
    34681 atccgcttaa tccaaacacc tcaaaataag gttttaccat tgacctagct ttactctttt
    34741 tttctttttt taaaattagt ggtagtgaca cattatctat tatagtttta tatggcaaga
    34801 gtaaatcttt ttgatacata taactatact taccatttat attcactttg ccactatcat
    34861 aatctgtaag tttagttata atattaaaaa tagtactttt tccacatcca cttggcccta
    34921 atatagaaac aaactcacct tcataaacat caatacttat atcttttaaa acttcaatat
    34981 ctttataagt ttttgataca ttttctatag atacttttac ttttttattg tggtaaaaat
    35041 tcatttgtgt aagcatcact tgccttcata tccttatcta ttaatttata ttcttttaag
    35101 aatgatgtat agttgttcca tacactatct ttcatttctc cccatcgtgg tgcatcatct
    35161 ttgtacttgc ttgctaaata ttcttgactc tttaaagcta gtttttcatc tacttcaggt
    35221 gcatgtttta ctaaaatttt tgctgattct tctggatttt ttattgcata ttcataacct
    35281 tttgttgtag cttttaagaa ttttttagca agctctggat tatttttaag cacagtttca
    35341 ttagaaatta taagtggtgt ataataatcc aatctctcat ctaaatcttt tactggtata
    35401 aagtttaagt catatccaat taaatcagct ttaactgcat cccaaccttc aaatatccat
    35461 gcaaaatcta cagttttcat agctgcaaag aaatcatctt gaccagtatt aactatcttt
    35521 aacttattaa aatcagctcc atttttcttc ataactgctt taaaaacagc ttcttctgat
    35581 ggagagcccc aaccaccata agtttttcct tcaaaatctt tagctgttgt tatattcttt
    35641 tcttttggtg atgcaaaccc agaagtatta tgttgtataa cagttgctac agcttttatt
    35701 ggaagtggat cttcactagt ctttgcataa gtaacttgtt cttgatagct tataccaaag
    35761 tcagcttttc ctgttgctac taaagtttct gctccacttt caggtggttg tactatttca
    35821 acatccaaac cttcttcttt atagtaaccc ttatcaagtg ctacaaataa tccagtatga
    35881 tttgtatttg gtgtccaatc aagcaccata gttacttttt tagaagaagt atctttatca
    35941 ttgtttttgt ctttattgtt atttgaagaa catccagtta ttgttgccaa tgtaaaaaca
    36001 cttactaaac ctaatattcc taacttttta aaattcataa ttgcctccct caaatacctt
    36061 taatctatta atctatgtct tataacaact ttttttacag cctgtacaat tcctacaaac
    36121 attaaactaa atataactac taatatagta gatgcaaata ctttatcaag tgcatatgca
    36181 ctttttgacc taagcatata aacaccaagt cctttcgttc cacctagcca ttctgcaaca
    36241 gttgctgcca ttacagcata cgttgcagat attttaagtc cagaaaaaaa tttatccatt
    36301 gccataggaa atttaaggtg aatgaatgta ttaactttat ttgagtccat agtcttaaat
    36361 aaatttaaat aatctttatc aatattctca atgccatcta caaaacttat taatatagga
    36421 aaaaaacaag taagcattac catcaatact tttggcattg ttccaaatcc aaaccatatc
    36481 ataaagagtg gtgctataat tattgtaggt atcatctgtg taaccaacat aataggataa
    36541 agacattttt ttattattga tacaaaatcc atcaatattc caattaccaa tgatattata
    36601 attgcgacta taaaaccaag tattgcttct cctatagtaa ccacagcatg tatggataaa
    36661 ttttgataat ctttaaaaaa cacattaatt atatctactg gtgtgggtag tatatactga
    36721 ggaacttcta aaactcctac catagattgc caaagaataa gtataaacaa aaatgttgct
    36781 attggatata tactatcttt gactttatct atgatttcta agtttttcat ctatagtcag
    36841 acctccagct ttttcatcta ctctagctac tgttatgact ctttcacatc caagattaaa
    36901 agggatttta tgtacctttt ttaataattc aaaaacttca tcaaaatcac cttcaacagt
    36961 agtgctcatt gctcctattt cacatttaag accacttttt tcaatttcag caatacaagc
    37021 atctactact ttatacatct cttcttcacc tttgtaaggt cttagtggca ttactgctat
    37081 gtctgcgatt accatttaaa tcactccttt aaaaataatt aatacctact aggtatattt
    37141 tgttttttat taaaaaattt tattactaaa aagatattat atttaaataa aagaaaagtc
    37201 ttatccaaaa taggataaga cttattttta tcttaactgg tctccctaca ctggcattat
    37261 ccaaacaggt tcaaagggtc gagataaaat ctcctctcag cacattagct ccccgaaata
    37321 tattatatct tttctacttg attatactac acttatcata ttttaacaaa ttttttattt
    37381 atattagata ttttatccgt tcaaaaatat ctaatataaa ttgtacatta ttacaccaaa
    37441 taaattatct taaaatggta atttatcaac atcttccaaa actagattaa ctgaattttc
    37501 tagtttttat tttgctattt caagcatatt tggatgtata tcaattccat acaaatattg
    37561 tgctaaattt ctgttttcac tcatctaatt tgcaatcgtt acaaattaca gattagaaat
    37621 agcttttaga ctctacttat ctttatctac aaaaaatcct tcaataaatt cattaataat
    37681 attcagtaat ctaaaaataa acctctatct agatttttaa gatacaagcc ttttaggaag
    37741 tatcttttgt gataatataa aaattactca tcattatcat caaaatcctc agcaaccaac
    37801 attttagata agaatcaaac tctataatat aattatttta tccaatttat ttttgagaaa
    37861 taaaatattt ttttaatttt taaatttttt aatatgcacc ttgaaaaaag aaacgttttc
    37921 ctatattatg gtaatagtag aataaaacta aataggcaaa actagagaaa tttagtgacg
    37981 caaagctata gggactaaga cttatgaaat gagttatgtc agccagttgc caaagagata
    38041 ttttcttttt gtttttatga atgtttttta gggggatata tgatatatgt ttatggataa
    38101 aatggataga tgtacacata tactaactgc atatatatgc agttcacgcg attactgcaa
    38161 ttttatagat acacaattaa atgattttat actagaatat ggagagaatg tggtagaatc
    38221 ttgtttgcat caagtgatgg tattggtaag cagatataat taaagaaatt ttattttcta
    38281 gaaaaccctt tttagtttta tctacgtttt ataattcatt aaagaactaa ttaaaataag
    38341 cttcattata tatttttcat aaagtaaaca gttgagttta tctcatatac tgtttacccc
    38401 aattacataa ttctaataat atagattcta atgatttacc acgtttagta gacaaatatt
    38461 caacttttgg aggaatagtc ggataagaaa tcctcttaat aagtccatct ctttcaagat
    38521 actttaattc ttgtgacagt atcttatgac ttatatgaac aacttctttt tttaattgac
    38581 tgtaacgtat tggctcttgt tttaaaatat gccataaaat aattgccctc catttacttc
    38641 ctatcacagt catggtaaaa ccaattgaac aattaaaatc tttttgtctt tcttcaatat
    38701 ttggaaattc ttcaaaatta ttatctatat ccatctcaat cctctctttc aaaatagtaa
    38761 gtatctaact aaaaagtgca ttcttgttta gcgtactaat tcgtaatata atcttgattg
    38821 tagtatagag ccttacataa tactactttt ttagatgtat atgtttaaac tttaatatag
    38881 acatatgtta ttttattaat tttatttaaa cagataataa taaataataa tttatctcaa
    38941 gttttctatg tagataaact aatctaatgt ttagaaagga ttatataata tgaataaaaa
    39001 atttcgtctt tatcaagttg actcattaca aaagaaaaat ttacaggtaa tccagctggt
    39061 gtaattacaa atgctgatgg cttatctgaa tcacaaatgc aaaaaatagc aagagaatta
    39121 aataattcag aaactgcttt tattttcaat aacttggcta aaaatcatca agaaaattat
    39181 gatgtacata tcagattttt tacactaact aatgaggttc ctatatgtgg tcatgctact
    39241 attgctgccc attatgttag agcagtagaa aaccaattca ataacaaaac agtatttcaa
    39301 aaaactagtg ctggaatatt accagttgat attataaaaa aagataatga ttatgaaata
    39361 attatgacac aaggaaaaat tgaagtatct ccaccgttac aacaaaccta tcaaaaaaga
    39421 ttacttaatg catttggtat tttaaaggaa gatattcgta cagattgtcc tattgccata
    39481 tcatctacag gacattcaaa agttatgatt ggaataaagg aattatcaac attaaatagt
    39541 ttatctccaa atttatatga attatcgaaa ataagtcaag aaataaaatg taatggatat
    39601 tatattttta cattacataa taaagaaact ctacaagttc atggaagaat gtttgctcct
    39661 gcaattggta tttcagaaga ccctgttaca ggaaatgcta atggaccctt aggtgcgtac
    39721 cttgtacatt ataaagtaat acctacaccc aacacagagt ttatatttga agcagttcaa
    39781 ggagaaacaa taaaacgcaa aggcaagatg ttagtaaaag taattatttt agataataaa
    39841 ccagttgaag taaaaataat tggaaaagct gtaattgcat tctcaacaaa catagaaatc
    39901 taattataga ttccttctaa gtaaattttt gaacttttta atactaattt aataacaaac
    39961 taatttatat acaatcaaat taattttttt attatttgtt aatatat
//
LOCUS       DAMJWU010000027        38629 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMJWU010000027 DAMJWU010000000
VERSION     DAMJWU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.027"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            95..997
                     /locus_tag="PC460_001548"
     CDS             95..997
                     /locus_tag="PC460_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2600436.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
     gene            complement(1196..1531)
                     /locus_tag="PC460_001549"
     CDS             complement(1196..1531)
                     /locus_tag="PC460_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600437.1"
                     /translation="MTHMSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVA
                     VWKPSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYS
                     CDLQQNKTT"
     gene            1481..1690
                     /locus_tag="PC460_001550"
     CDS             1481..1690
                     /locus_tag="PC460_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600438.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSRPNETDLIELKNAISK"
     gene            1783..4446
                     /gene="mgtA"
                     /locus_tag="PC460_001551"
     CDS             1783..4446
                     /gene="mgtA"
                     /locus_tag="PC460_001551"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF2600439.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            4462..4965
                     /locus_tag="PC460_001552"
     CDS             4462..4965
                     /locus_tag="PC460_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2600440.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            5103..5780
                     /locus_tag="PC460_001553"
     CDS             5103..5780
                     /locus_tag="PC460_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2600441.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            6027..6338
                     /locus_tag="PC460_001554"
     CDS             6027..6338
                     /locus_tag="PC460_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2600442.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            6504..9269
                     /gene="mgtA"
                     /locus_tag="PC460_001555"
     CDS             6504..9269
                     /gene="mgtA"
                     /locus_tag="PC460_001555"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF2600443.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            9282..9449
                     /locus_tag="PC460_001556"
     CDS             9282..9449
                     /locus_tag="PC460_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600444.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            10500..10799
                     /locus_tag="PC460_001557"
     CDS             10500..10799
                     /locus_tag="PC460_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2600445.1"
                     /translation="MKHLHIQQLSTIGSSLKKHLNSTEHNAPICGFLQHITGIICLAQ
                     DQYVCIALVLFYCIKLFQKIFAVGYQIPPFMVSVSAKRGRSLFALATFAGKGVRL"
     gene            10796..11212
                     /locus_tag="PC460_001558"
     CDS             10796..11212
                     /locus_tag="PC460_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2600446.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGAMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            11217..11456
                     /locus_tag="PC460_001559"
     CDS             11217..11456
                     /locus_tag="PC460_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2600447.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            11775..11918
                     /locus_tag="PC460_001560"
     CDS             11775..11918
                     /locus_tag="PC460_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2600448.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            11981..13741
                     /locus_tag="PC460_001561"
     CDS             11981..13741
                     /locus_tag="PC460_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2600449.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(14230..14868)
                     /locus_tag="PC460_001562"
     CDS             complement(14230..14868)
                     /locus_tag="PC460_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600450.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(15278..15454)
                     /locus_tag="PC460_001563"
     CDS             complement(15278..15454)
                     /locus_tag="PC460_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600451.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(15508..15604)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16401..16802
                     /locus_tag="PC460_001564"
     CDS             16401..16802
                     /locus_tag="PC460_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2600452.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            16799..17866
                     /gene="rsgA"
                     /locus_tag="PC460_001565"
     CDS             16799..17866
                     /gene="rsgA"
                     /locus_tag="PC460_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2600453.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            18402..19100
                     /locus_tag="PC460_001566"
     CDS             18402..19100
                     /locus_tag="PC460_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600454.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            19278..20168
                     /locus_tag="PC460_001567"
     CDS             19278..20168
                     /locus_tag="PC460_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600455.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(20561..21517)
                     /locus_tag="PC460_001568"
     CDS             complement(20561..21517)
                     /locus_tag="PC460_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2600456.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            22220..23215
                     /locus_tag="PC460_001569"
     CDS             22220..23215
                     /locus_tag="PC460_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2600457.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            23193..24056
                     /locus_tag="PC460_001570"
     CDS             23193..24056
                     /locus_tag="PC460_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2600458.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFLKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            24053..24847
                     /locus_tag="PC460_001571"
     CDS             24053..24847
                     /locus_tag="PC460_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2600459.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGIFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            25132..26511
                     /locus_tag="PC460_001572"
     CDS             25132..26511
                     /locus_tag="PC460_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2600460.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
     gene            26959..27672
                     /locus_tag="PC460_001573"
     CDS             26959..27672
                     /locus_tag="PC460_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600461.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(27944..29482)
                     /locus_tag="PC460_001574"
     CDS             complement(27944..29482)
                     /locus_tag="PC460_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2600462.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            30364..30819
                     /locus_tag="PC460_001575"
     CDS             30364..30819
                     /locus_tag="PC460_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2600463.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            30868..31452
                     /locus_tag="PC460_001576"
     CDS             30868..31452
                     /locus_tag="PC460_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2600464.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(31457..31780)
                     /locus_tag="PC460_001577"
     CDS             complement(31457..31780)
                     /locus_tag="PC460_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600465.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            31932..32450
                     /locus_tag="PC460_001578"
     CDS             31932..32450
                     /locus_tag="PC460_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2600466.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(32626..33294)
                     /locus_tag="PC460_001579"
     CDS             complement(32626..33294)
                     /locus_tag="PC460_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF2600467.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            33409..34824
                     /locus_tag="PC460_001580"
     CDS             33409..34824
                     /locus_tag="PC460_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2600468.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            35047..35505
                     /locus_tag="PC460_001581"
     CDS             35047..35505
                     /locus_tag="PC460_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600469.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            35502..36086
                     /gene="clpP"
                     /locus_tag="PC460_001582"
     CDS             35502..36086
                     /gene="clpP"
                     /locus_tag="PC460_001582"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2600470.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            36398..37474
                     /locus_tag="PC460_001583"
     CDS             36398..37474
                     /locus_tag="PC460_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2600471.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            37541..>38629
                     /gene="bclA3"
                     /locus_tag="PC460_001584"
     CDS             37541..>38629
                     /gene="bclA3"
                     /locus_tag="PC460_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF2600472.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTGVGITGATGATGATGPTGADGATGPTGA
                     TGNTGADGVAGPTGATGATGNTGADGATG"
BASE COUNT        13397 a         5254 c         7059 g        12919 t
ORIGIN      
        1 gacccgatag gatatacaga cctaagtagt agttactggc aacagcactt aatcggtgca
       61 ggacgagtaa aacaatagaa aggacttgaa aaatatgttt aagaagaata agaaacagac
      121 agaaaatgtc aaagaaaaaa aggtacgtac tgtcaaggta ggcacacata agaaaaccgt
      181 gattgcattg tgggtggtgc ttattgcaag cgtgagcttt ggagtatata agaattttac
      241 cgctattgac cagcacacga cccatgaaaa agaagtgatt gagcttcgct tgcaggacac
      301 caacgggata gaaaatttcg tgaagaattt tgcaaagtcc tattacacat ggagtaacag
      361 caaagaagcg attgacgcaa ggacgcaggc aatcagcggt tatctgacaa aggaattgca
      421 ggacttgaat atggatacca ttagaacgga tataccgacc agctctgcca ttacagatgt
      481 gattgtatgg agcattgaac agtctggaac ggacactttt tccgctacct acgaagtaga
      541 tcagcagata aaagaggggg aacagacaac agcggttaag gcaacctata ccgtaaaagt
      601 tcatgtggac gctggcggga atatggtaat tattcagaac cctacccttg caccagcaat
      661 cgagaaatca gactatgaac caaagacacc agaagcagac gcaagtgtag acgcggatac
      721 cataaatgac gctactgcat ttctggaaac tttctttaag ctatacccaa cagccacaga
      781 aaaggagctt gcttactatg tgtctggaaa tgtgcttgag tctatcggca gggactacct
      841 ctattctgaa ttggtaaacc ctatctttac ggttgatggg gacaatgtga aagtcaaggt
      901 tgccgtgaaa ttcattgata atcagacaaa ggcaacacag gtatcgcagt ttgaattagt
      961 gctacataag gagagcaatt ggaagattat aggataatta ttgaaagcgt gcatttcttt
     1021 aggaatttgc acgcttttgt taagagcatt attctttata tttatgaaag acatcttaaa
     1081 caaagtatta atggcttcgc aaaacttttt tcttaatata tttggattag aaaaagcgca
     1141 tcttaattgt agacttacta tacatatgaa ttatgccagt caacgtattt aaaatttatg
     1201 ttgttttatt ttgttgtaaa tcgcaagaat agataagagt aatcaatggt gaaggggaat
     1261 tattaaaaat actattggtt tcttctgtcc cgttccaatt tgcgcattgt attttttcat
     1321 aaattacaga gtggtcaatt atgtagttca gatattccat acaaactaat tgtattgttt
     1381 ttacactcgg tttccatact gccacttttg tcattctatc actcataata caaatattat
     1441 tatgagcaac ttttctaagt agttcattta aatttgatac atggtcaagg tgaaaaccaa
     1501 gaagaagttt gtcagcactc atatgtgtca aggggaaatg cctatttatg tgcgagagcg
     1561 ctatgagaaa atgcttgccg tatccatccg tgctccaaat ttagaaggaa tggttgaaaa
     1621 ctttgataag gatttatctc gccctaacga gacggatttg atcgagctga aaaatgccat
     1681 atcaaagtaa attgcaagaa aaaagcaaga atataaatat agcattttca gaacactgtg
     1741 ttaaaatgtt attacttata cttataaagg aggggatgtt tcgtgtcaaa agcaacacta
     1801 tttgatagtc gtatcaaaaa atacgcatac tgtagaacat cagaaattta tcgagacatt
     1861 ggctcatctc cggatggttt atccattgag caaatcggtt caatgcggga gaaatacgga
     1921 gcgaacagct tcaatgggcg gaaaaatgat acgacgatgc aacggttgag acgggcattt
     1981 attaacccgt ttcatgtgat tctttttgtt ctgggcattg tttctcttgt tacggatgtt
     2041 ttcgtagcat ccaattttgc gagaaatgct actaccgcca ttatcatctt ttccatgatc
     2101 gtaattagtg gcgtaattcg tctgattcag gagctgcgag caaagagcgc tgctgctcag
     2161 cttgatcgcc tagttcatga aagcgtcaca gtgaggcgtg atggaaagtt aatagagatt
     2221 cctggcgaag aattagtggt aggggatatt gtactattct ctgcaggtga tcgtgttccg
     2281 gcggatatca gattgacgaa gattacggat ctctttatct ctcaggctgc gattactggc
     2341 gagagcgcca tcattgaaaa gagctgccga aaactcagtt acggagagca ggatacactg
     2401 actcagcttg aaaacctcgc ttttatggca accacagtca tcagtggaaa aggggaaggc
     2461 attgtactgg cagtaggcaa agataccctg tatggaggat ttaccaaacc ggattctgat
     2521 gataaaaatt cctttaaaaa aggagccgat tccatcgcct gggtgatgat acgttttatg
     2581 gcgatattag ttcccattgt atttatatta ctggggatta ctgatggaaa atggatggaa
     2641 tcttttgctt tcgccctgtc agttgctgta ggtctgatgc cggaaatgct tcctatggtt
     2701 attacagcct gccttgccaa aggaagtctt gctatgagca aaaagcagac cattatcaag
     2761 gatattaatg ccatgcagag ctttggtagc atggatgtgc tttgtatgga taaaacagga
     2821 acacttacca atgaaagcat cctgctggaa tattatatgg atatttttgg gaatgaaaat
     2881 gcagaggttc ttgatcttgc ttatctgaat agctgctatc attctggtgt ctgcaatacc
     2941 attgataatg ccattcttgc ctgcaatacc atgccagggc gggaagccca ttacgcaaat
     3001 cttctgacaa aattccaaaa agctgatgaa attccctttg actatgctcg taagtttgtc
     3061 agtacacttg tacaggatag caatgggaat agtcagctta ttatgaaagg tgatattgaa
     3121 catatattct cacgctgcaa ccatgtagaa tatcgtggaa aacttctccc catggggcag
     3181 gatgcgagac agagtgtatc ttctgtagta ggtgaaatgt tacaggacgg aatgaaggtc
     3241 attgcggtgg cacgaaaaaa tgtgggagcg caaaagaaaa taactcctga tgacgaatgg
     3301 ggcatgactc tagttggcta tctatccttc tttgatgcac caaagcagac agcaaaggag
     3361 tctgtaactg cactcaaaag attaaaagtt atccctaaga ttctgacagg ggaccaggca
     3421 gcgattgctg tgtctgtttg ccgccgggtg ggtatttata cagagcgtat cttaactggt
     3481 actcaactgg atgaaatgac agactgtgaa cttaaaaagg ctgtggaaca tactcatgtt
     3541 tttgcagagc ttactccggg acataaggtg cgtcttgtct ctgctatgca ggataatggt
     3601 cattccgtgg gatttcttgg tgatggagtc aacgacattc cggcactgaa tgaagcaaat
     3661 gtgggaatct ctgtagatac ggcagtggac gcagcaaaag atgctgccga tgtggttttg
     3721 ctgcaaaagg acttgaatgt tttggagcaa ggtgtaatgg aggggcgaaa aacttttacc
     3781 aatatgctga aatatattaa aatcaccgct agctctaact ttggaaatat tttttccatc
     3841 atttgtgcca gtgccttttt accgttcctg cctatgactt caattcagat tctgcttttg
     3901 aatctgctgt atgatacgtt gtgtattgtg cttccttggg acaatgtaga tgaggaagag
     3961 atgctgtccc caagagactg gtccggcaaa acactgagac agtttatgct gtctttcgga
     4021 ccgatcagtt ctctgtttga tattgtcacc ttcctatttt tgtattattt cctatgtccg
     4081 atgctatgtg gaggggcaac ctatctgcag cttacagatc cttccctgca acttcagtat
     4141 gtagcactgt ttcagactgg atggtttttg gaatctatgt ggacacaggt tttgatttta
     4201 cattttctgc gaacttccaa gattcctttt ttccagagca gggcatctgc accggtcatt
     4261 tctataactc tagtggggat tattgctttt acttccctta catttgtcgg cggtgcgtcg
     4321 ttgtttggac tgacgaaatt acctctttgg tatttcgtat ttttactgct ggtggtatct
     4381 ctctatatgc tgctgaattc agtagccaaa tatttttata agaaaaaata tcaagagttg
     4441 atttaagagg aggcattcat gatgaaaaag aatattagtt atattccgat ggattcggct
     4501 cttgaaatct ggctattaga aaaactacta agagatccac cggacagtga ttgtgcgaag
     4561 gatttacttt ctggtattca ggagatgatt cgtggagagg tatcttcgtt attgttagga
     4621 gcatcagaag aaagcgattg gccagtgggt actctgtcag atagaatcct ggcaggggat
     4681 ctggaaattt atccaagagg cagaaaagta ctgataaaag gttctgaagt caacctgact
     4741 ccaaaggaat ttgatatttt gtactttctt gtccagaaca aaggtgaagt ttttacaaag
     4801 gaacagattt atcgtgccgt atgggaggaa gattatattc tggataacag caacattatg
     4861 gcttttatcc ggaagttgcg aaaaaagatt gaacctgctc cggattgtcc cacatatatt
     4921 ttgaccatct ggggtattgg atacaaattt aatgatcaga tatgacaaag acggactaag
     4981 accattttcg gtttcagtcc gtctattttc ttaccaaatc gcaagaaaat cgcaagactt
     5041 tagccatagg attgtatttg gattttttat atactgttag tagttaaagg gaggtgtttc
     5101 atatgtttta cacagatttt attttgagaa ttgccctctc cttaacactt ggatttctta
     5161 ttggattaga acggcaattg actggtcatc ctgcaggtat tcgtatcaat gtgttaatct
     5221 gcatgggtac aagtttcttt accttgtttc ctatgctcta cggttcggat caggtattcc
     5281 gtgtcggaag cagtattata tccggtgttg gttttttatg cagtggtgtt attttcaagg
     5341 acagagggac agtacgtgga atgaataccg ctgccacctt atggtgtact gcagcgattg
     5401 gaattcttgc aagtacggga atgtatgcaa tggcgatcac cgccgctact attttaattg
     5461 gttccaacct gattctacgt ccctttgcaa gaaagtttca cccaattact tccagtgatg
     5521 aatctgaaaa gcagtatcgt atttcggtta catgtcagga aaaggcagag caggatatac
     5581 gcttattgct gattaacagc aattcctgta agacgctctt tttgaacaat ctggaaagcg
     5641 gtgatgtggt tggtgacaag gttgagattg tcgcagaata ctgttctgtc ggaaaatcca
     5701 aaaacaatat tttagaagga atcgttgggc aggtattggt aattccggaa gtgattagtg
     5761 ctggttggga ggtgttgtga gtgaggagag aaatcgaaga tttgaaacga gcaattcttt
     5821 ttgctttcta ccctgtattt acaatcttat gtagctattt gggttggaat ttctttgaac
     5881 aaagaatggt aatctttaag atatggtatc aggtgagtat tgtgatctgg tttattcttg
     5941 ctgttactat tattgtgctc ttcattaaac ttctgaagct gacaaaacta agaaacaggt
     6001 ttaagcattg aaaggaggaa caaacgatgg attttacatg tatctttttt ggaattttat
     6061 ttactcttgc aggcttttgg ttggcctttg gaaaaggata tcgtcatctt tccctgtgga
     6121 aaaatatgcc gcaggaggag aaagataaaa ttaatattgt cccgctttgt cgtaatgttg
     6181 gagaaatgat tgcgttaaat gggattatat tcttaatgaa aggatttttg ccgggatttt
     6241 caaaccactg gtttgtattt gcaataatcg catggctgat tgttgccggc tttgatgtct
     6301 ggtatatcga aaaaagtgtc cgctatcgca accaataatc cacattgagg tttcacatag
     6361 atattttttt aagattgtga ggtgaaagac atggactctg atgtcagtcc acgaaggcag
     6421 agaatattat atgagaattt ggagattccg tgtcgctcat ataatattcc ttgcccctat
     6481 gaaaattcaa ggaggtaaga ccaatgaaca aaaaagaaaa tcgtatggct attcgtcaga
     6541 ctgtagaaaa agcagtcatc cgtgacgaac agaaccgccg cattcaattt gcggcaacca
     6601 atcctattaa agaagtttta aaaaaacttc ataccacact tcgtggctta gaagtagatg
     6661 aagtatccgt aagtcgtgct aaatatggta ccaataaagt cactcatgaa aaaaagaaat
     6721 ctctggcaaa acgactggtt ggcgcattta tcaatccatt taccgccatt ttattttgtc
     6781 tggctttagt atccacaatt acagatatga tttttccgta tttttctctg tttggcagtg
     6841 tgccagagga ctttgattcc ctgactgttg taatcatttt aacaatggta ttcatttccg
     6901 gtacgcttcg ttttgtacag gaatccagaa gcggaaatgc agcagagaaa ttgttagcca
     6961 tgattacgac cacttgtaca gttactcgta gaaactatga aaaaaaagaa cttcctatgg
     7021 atgatttggt ggtgggagat attgtgcatc tttctgctgg agatatgatt ccggcggatg
     7081 ttcgtatttt ggatgcgaag gatttgtttg tcagtcaggc aagtcttact ggtgagagtg
     7141 aacctattga aaaaacaccc aatgtaagtg ctcagaaaga aagcgtgact gattacacaa
     7201 acattgcttt tatggggagt aatgtaattt ctggtagcgc aacagctgtt gttgtttgtg
     7261 ttggcgatca tactctgttt ggctctatgg cgtccgcagt tgctggagaa gcggttgaaa
     7321 caagctttac caagggtgtc aatgccgttt cttgggtatt gattcgtttt atgctcatta
     7381 tggttccgct ggtgttcttt gtcaatggta tcaccaaggg cgattggttg gcggccttct
     7441 tattcggcgt ttctattgct gttggtttga caccagaaat gttgccgatg attgtaacaa
     7501 cctgtcttgc gaaaggcgct gtttccatga gcaagaagca gacaatcgtt aaaaacctca
     7561 attccattca gaacttcggt gctattgata ttctctgtac tgacaagact ggcacgctga
     7621 cccaggataa ggttgtgctg gaataccatc tgaatgtaaa tggcgaggac gatacgcgag
     7681 tactgcgtca cgcttacctc aatagttact tccagactgg ctataaaaac ctgatggatt
     7741 tggcaattat ccataagacg gaggaagagg aagccgcaga ttcgcgtctc attgacctgt
     7801 ctgaaaacta tgtgaaagta gatgagattc cttttgactt cacacgtcgt cgtctgacta
     7861 cagtggtaca ggataataag ggtaagaccc agatggtaac aaaaggtgct gtggaagaaa
     7921 tgctctctat ttgtgcgttt gcagagtgta atggaggagt gcagcctctg actgatgacg
     7981 ttcgttacag aattctggag acagtggaca atcttaacga taagggattc cgcgtactag
     8041 ctattgcaca gaagagtaat ccttccccaa tcggcgcatt tggtgttaaa gatgagtgtg
     8101 atatggtact gattggatat ctggcattcc ttgatcctcc aaaagaatct actgcggatg
     8161 ctattaaagc tctgaaagac catggtgtta taaccaaaat tttgacaggt gacaacgaca
     8221 aagtgactcg aactatctgc aagcaggtgg gattagaggt tcgcaatatg cttctcggtt
     8281 ctgatttgga acaaatgggc gatgcagagc ttgcaagagt ggcagagtct acagatgtat
     8341 ttgcaaagct aacccctgat cagaaagccc gtgtggtttc tgttctccgt gaaaatggtc
     8401 atacggtcgg tttcatgggt gacggtatta atgatgctgc agctatgaaa gccgccgata
     8461 ttggtatttc ggtggatacc gccgtggacg tggcaaaaga atctgctgac attattctat
     8521 tggaaaaaga tctgatggtt ttggaacagg gtattattga gggacgaaag acatatgcca
     8581 acatgatcaa gtacattaaa atgactgctt catccaactt cggcaatatg ttctcagtgc
     8641 ttgctgcatc tgctttgcta cctttcttac caatgatgag tgttcatctt atatttctaa
     8701 acttaattta tgatttatcc tgtactgcaa ttccttggga taatgtggat gaggagttta
     8761 ttaccaaacc tcgtaaatgg gacgcttcca gtgttggaag ttttatgatc tggattggac
     8821 caaccagttc tatctttgat tttacaacct atatttttat gtactttgta ttttgtccat
     8881 tgttcgtatc gaatggtgta ttatttaatg atttgcctgc tcatttcagc ggtgcagaac
     8941 tggcaatgct tcaggcacag tatatcggga tgttccaagc agggtggttc gtggaatcta
     9001 tgtggagcca gacattagtc attcacatga ttcgtactcc aaagctacct tttgttcaga
     9061 gtcgtgcatc agctccggtg actttgctaa caatgactgg aattatagtt ctgaccatta
     9121 ttccgtttac tgtgtttgga actatgcttg gatttgttgc attgcctgca gcatactttg
     9181 cttatctgat tccatgtatt ctgctgtata tgactttggc aacaagttta aaaaaagcct
     9241 atgtccgtca tttcggagaa ctgctttaag gaggtggaat gatgaactgg aaagaacttt
     9301 ggatgaccct ttttggaact accgaatggc ttggtcttaa tatcggcttt tgggtggcta
     9361 tggcagttgt gttgctgatt gttattgtaa tgaatgtagt attttggtcc atgaaaccaa
     9421 aaaaagacac aaaagagtct tataaataag atatcagtat gaatgaatca agggagatgc
     9481 acagtttttt attgtggaat ctccctatca tatcaaggcg cattcaattg tggcaattta
     9541 gtagtaaagt atgtgttttt ttatcattga attacctata aatgctgtat ataagtggat
     9601 aattgtaatt tcgatataaa aattatggta cttaaaagaa atttcgtttt ttattgtttg
     9661 tttacgcgtc tataacgaaa tcgtgaaata atattcaaag gtagatttta ttatggaatg
     9721 tatgtctgta gaagaagctt cgataaaatg aggagatatc tgaaagaagg ataaaaaaat
     9781 tatgtaatgg aaacagaatc ccaaatctat tgtaacaaca atgacaggta atgaatggtt
     9841 acgtagattg ataattccta ttcaaattat tatctatctt atattgagtg gtgttttgta
     9901 attacatttt tatacaaaaa gaatatgttg aacgcacaat gataaatatt ttgtattccc
     9961 caactaatag aataaagttt ttgcttggaa agtatgttat ataggcatta tggtttgtaa
    10021 tgatcacgtt gacagattat accgctatat atgaagtcaa agaacttaaa tcatttacaa
    10081 gtgagataat ctatctaagt ctaaaaaact ggatttctca atctattgtc aatgagtcca
    10141 ttacttatta tttgtgtttt gcaaagaaat gttttctact ctagtttact ttttagttac
    10201 taaatatcgg gaattggttt tgttggacta tattgatctg aaagtataca aaatattatt
    10261 ccgtggtctg ctgtaacccc tataagttta ttagatgcac aaggattgct accttatatt
    10321 tcaattctac tttgttattt tgcaagactg attataaata catactgctt tagagggttt
    10381 agtttagttc tttttgaact tttgaataag acgacggtta tatagccgcc gttttatttt
    10441 tgcctaacgt tggacaaatt actggatggt cttgttttat cggctctagg ggtagccgta
    10501 tgaaacactt acatattcaa caactttcga ccatcggtag ttcattaaaa aaacatttaa
    10561 attctacgga acataatgct cctatatgtg gttttttaca gcatataaca ggaataattt
    10621 gtttagcaca agatcagtac gtctgtattg cccttgtgct tttttactgc ataaaacttt
    10681 ttcaaaaaat ttttgcggtt gggtatcaaa tcccaccttt catggtgagt gttagtgcga
    10741 aaagaggtag aagtcttttc gctttagcaa ctttcgctgg aaagggggtg agattatgaa
    10801 accatcttct ttcgagggcg ctatcagact tcaatttgat tgtctagctc gcaaggttgt
    10861 aggacgaact gtaaagaact atgaccgaga gcttggcaga cgtgcaagac gtgaaacgcc
    10921 gttctgtgat ttatctgaaa tggagttaaa ccagatcggt gccatggacg aatattgcgt
    10981 tgagtttacg tcatttgatg tatttggttc agaagtccat atctatgatg aacgcttatg
    11041 tgaagctatc aaagaattaa cagaaagccg acgtaacatt ctgcttatgt cctactttct
    11101 tgaaatgacc gacgctgaaa ttgcagaggt tatggaaatg gaacgatttt ccgtatgtag
    11161 aaacaggctg cacacattaa agcttattaa ggatatgtac gaggaggaat aaaaacatga
    11221 tgaagtctac aaaaaagtgt ccatcattct cgaccatcag ttctgcagct gacggtaacg
    11281 aggtggcaat tgaaaagatt ttgaaccatt atgacgctta catatcaaaa gccagcctac
    11341 gcccgttcta tgacgaacac gggaatatgt atatcgtgct tgatatggaa ctgaaaggca
    11401 ggatacgggc agcgttgatt aaagtaatct tagggtttga agtcaaagtg aagtaaatat
    11461 aacagagagt atctaccacc tcattttaac tcttttccgt tctttgacaa gtgaatacag
    11521 taatcagata cgtttgatat acgacgagcc agcggactgg aacgccacga ccactgaaat
    11581 ggggaaagca gcgagcgatt atgccagtga accgcaagcg atctttggaa aggttgctgc
    11641 catgactcgt atatctgaat aatgatacac ccgtatgaca cggttcaccc atcagaatgg
    11701 gaatggtgaa agtccagtgg agctgccgaa gccgtctgat tacttccata aataaaaaag
    11761 caaggagggt tttaatgaat aatataatta ggtatagtat gcaaattgct atgttaaaac
    11821 aactgcttgc ccgtaaactg atttctgaaa aagaatacac tcttgtaaaa caaaggctga
    11881 tgaaagacta taaggtgctt tctgacataa ctgcttgaat tgtgtttttt aaagtagtac
    11941 aataacaata tgaaaggaac acaaaataag gaggtgtact atgcaggaag ttgaagtaat
    12001 aaaagcaaaa aatgttttgg caagacaagc ccgtggaaaa gccattgagc gtttgcgtgt
    12061 agctgcatat tgccgtgtaa gtacagatag cgaagatcag ttaaacagtt ataaatcaca
    12121 agttcagtat tatactgaca tgattaagaa aaacaaggaa tgggtgttag ctgacattta
    12181 tgctgatgaa gcaattacgg ggacacaagt aacaaaacga gaagattttc agcgtatgat
    12241 aaatgactgt atgaatggtg aaattgacat ggtatttaca aaatccatat caagatttgc
    12301 cagaaatact ttagacacat tgaaatatgt tcgtatgcta aaagagagaa atatcgccgt
    12361 ttattttgaa gatgaaaaaa tcaatacact tacaatggat ggagagttat tgcttgttgt
    12421 tcttagttct gttgcacagc aggaagtcga gaacatttca gccaatgtaa aaaaagggtt
    12481 gaaaatgaaa atgaagcgcg gcgagctggt aggttttcaa ggttgtattg gatatgatta
    12541 tcacccagag gataagacaa taactgtaaa tcaagaagaa gcagagattg tcagatatat
    12601 tttcaaccgt tatgttgatg gtgctggtgg ttctgttatt ggacaggagc ttgaaagtct
    12661 aggttataaa acgaagtatg ggagttccac atgggcacca tctacggtta ttggaattat
    12721 taagaatgag aaatacaagg gtgatatact cttaggcaaa acttttactg ttgatccgat
    12781 ttccaaacgt cgccttgaaa acatgggtga agaagataaa ttttatatga aagatcacca
    12841 tgagccgatt attagtgaag aagtttttga aaaagcacag gaaattctta atcggagaaa
    12901 taagaaccga acaacggtag gcagcggtaa gcgtgagaag tatagcagga aatacgcatt
    12961 tagctgtatg ctagaatgtg gcttttgtgg tggtacttta acaagacgta attggcacag
    13021 tggctcaaaa tacagcaagg ttatatggca atgtgtaacg gctacgaaga aaggaaagaa
    13081 attttgtaaa catagcaaag gtattccaga aacagctatt gaagaagcat ttgtagaaag
    13141 ttatcgttta ttatgtgatg ataataaaga tgttctggaa gaatttttgc agagaatgga
    13201 cgatactctt agtagtagtg tggtgtcaaa acaacttgca aaagcggaaa aggagataga
    13261 cgcattagag aagaaaaaaa gtaaacttgt ggatatgcgt ctggaagaaa ttattgataa
    13321 ggaaacctac gaaagtaaat atgctgattt agtaagtaaa caagaacagc ttgttgaaga
    13381 aagacaaaag ttacaagaaa cttctgataa tgagaaagac ataaaaaaac gtttgaaaga
    13441 gtttaagaaa actctggaac agaatgaggt tcttgataaa tttgaccgct atgtgtttga
    13501 aagcattgtt gaaaaagtaa ttgtaggtgg acttgatgaa aacggaaatg ttgaccctgc
    13561 acagttgacc tttgtttata aaacaggctt gaagaatagc gtagatggtg ctaaatttaa
    13621 aacacaaagg aaaaatgcaa gaggacggca taggactaat gaattgtgtt cacatgacag
    13681 caacgaggtt gataaaatgt gttccgatag tagtaacgac acatgtggaa actgtagcta
    13741 agataatcaa acaatagtaa tgtttatata gattatatac taataatata ttgtatttgc
    13801 caccgatttg ccactgtaaa gcgatttcgg tggcaaattt agtctacaat gttatcaaaa
    13861 atctctaaag ttttattttt aattctataa aacttattat aattgtaatt ttaaaagaaa
    13921 tagtcaatct acagtagcca gattttatac aactcatttt tttcatagag taatttacat
    13981 aatctatagt tgaatattat ttaatatttt tataattcat tagcaaatat aggcattaca
    14041 aatgttgata aacatgtgaa agcacatatc cacttataat tatttttttt aaaaccataa
    14101 aatcacctct tcatttgttg tatgttttta taacaaatga agaggtgatt tccagacaat
    14161 ttaaaaggtt tttgtatatt gactaaaatt tatttttatt atttataatt cttaataata
    14221 catatgtacc tactgattcc atattaaact gtataatttt tgttcatctt tttttataat
    14281 taaaactacg tagttctcat aatttctctt atatatatac ttatcatctt ttttaacttc
    14341 taacttatat tttttaaaat cttcttcaat tttattttta ttacttttat taatgttaac
    14401 agaatctctt ttaaatttat caatatattt ttctatttca ttaatattat ctttgcttac
    14461 aactatacca ccttttaaat taaaaacttt tttgaaattt ttttcagaaa atccttgaat
    14521 aaaataactt tgtccatctc cattaatact atcttgagta tcaaatactt caatttcttt
    14581 gttaggtatt ggtatatcaa aattccaaga ttcctttagc attgaaatat tagctgtttt
    14641 tcctaaaaaa agattatata ggatacttcc taataacaaa acacctatta atagtagtgt
    14701 gattaataca atttttctct ttttcatttt tcgttcacaa ccttttgaaa aaacatgatt
    14761 taattcatca tttgaaggca tgtcttcaag aataattttt gttgtgatag gaatgctttc
    14821 ttgaaacata tcatttgtaa tatcaaaatc ttttctttga ttactcaata tccatttctt
    14881 ctaatatttc ttttgatttt ttctagtaca ttgaatactc ttatatacat tctcctcttt
    14941 taaaattaaa agttataaaa gaataagtta tataaaaaat tattatattt tttaatatag
    15001 cttatttgca aatttataat tttgttaatc taatccttca aactaaaaaa tcatttatat
    15061 ttacagaatt tcattttaag agatgtttat tttcacatag ataagagaat ttaagttctc
    15121 atatttatta tttgatacaa atttacatct ttattatatg tattttaaat aaaaaataaa
    15181 attatgaata ctagaaatct atcttaatta gctttctaac ataaggctat ttaaagtaga
    15241 taaaactaaa aagggttctc tagaaaacca aatctattta attatactta cttactaata
    15301 ccatcacttg atgcaaacaa gattctacca cattttctcc gtactctagt ataaaatcat
    15361 ctagttgtgt atctataaaa tcacaataat catatgaact accaatataa gcagtcaaca
    15421 tatgtgtaca tctatccatt ttatctctaa acatatatat tattatcccc ctaaaaaact
    15481 ttcataaaaa caaaaagaat atctcttttt ggcaactggc tgacataact catagtttat
    15541 tttataagcg ttagtcccta tagctttgcg tcactaaatt tctctagttt tgccgattta
    15601 gttttattct acaattacaa taatacaata gaattgctta ttattcaata tacttatttg
    15661 aaaattaaat aaatttacaa atatgaacgt tattttatat caaaaaaaat agactatgca
    15721 tgtagaatat atggaaaaac ttgattgtag gttttgtatg aaaagacagt tgttattaat
    15781 ttaagtgaga taaattgagc aacaggcata aaatgagaaa acaaataata gcagttaatt
    15841 tttattatta atactaggtg aacattaaat ttcaaaatac cactagattt tatagaataa
    15901 gaaaatgaga caaacataaa tatatgaata tattaaatta tacttgaaca tacttatatt
    15961 ttatgataaa atttcaataa taattattag tgaaggagtg tcttgcctga tgtcagagtt
    16021 atatataatg gtaaccaaca aataaatagt tggagacgca tatgggttct gtttaatcag
    16081 aatttttaag tgtatttttg atacatttga ttgttgtgtc ttactctgcc tttatagata
    16141 actaacatta gacaacaaat aataatatta catattactt tgttaaattg tggtgtagtg
    16201 ggctttaatc tgctacactt ttattaattt tacacagtat tattttgaat ataattatgt
    16261 tgaaatagaa gatactaaat tttctagtcc acaggggttt tctgtttttg tgtaggtaaa
    16321 tatgtatacc acataaaaag gcagatataa atacctgtgg actttttttg tagaaaatac
    16381 acgatacgga ggaaataaaa atgaattata aaaatagaaa acgagcttat tatcaggaaa
    16441 attcctgtat ggataacttt atttgcaaaa attgtggttg ccttgtagtt cctgaaggag
    16501 ctggtacaca acatagaaat cattgtccac attgtttaca tagtcttcat gtagatatca
    16561 ttcctggtga ccgtgctgca gattgtgatg ctaatatgga acctattgga gtgtgggtga
    16621 gaaaaaatgg tgagtgggca attattcatc gttgtacacg ctgtggacat cttagttcta
    16681 atcgtattgc agctgatgat aattcagtga aattaatgtc aattgcatta aaaccactgg
    16741 cacagccccc tttcccacta gaaagaatca ttgaaaagtt ggaaaaggag gaaacagaat
    16801 gaatggtatg aaacaaatca atttacaaga ctatggtcta aatagaaatt ttattactca
    16861 aagtatggaa tatcatgatt tataccttgc tcggattatc tcgcaggaaa agaactttta
    16921 tcaagctgta tgtgtaaatg gtgtactgtt tgcagaggtt tcaggaaagc tgcgttttga
    16981 ggctactata caatctgatt ttcccacagt tggtgatttc gttatgttgg atcgttgcaa
    17041 taataaaaat ggtaatgcta ttattcatca tgtgcttcat agaaaaagct tgtttgtgcg
    17101 taaggctgct ggaaaggtaa atgaggagca ggtggttgca gctaatattg acattgtttt
    17161 tttgtgcatg tcactgaata aggattttaa cattcataga ttggagaggt atctgtcttt
    17221 aacatgggaa agtggagcta tcccagtagt agtacttact aaatcagact tatgtgacaa
    17281 tgtgcatcgt aaattacaag tagttaattc tatagccttg ggtgtagatg tagttgttac
    17341 ctcatctata gagccagatg gttataaaca gatttctaag tatattaaac caggctgtac
    17401 tgttgcctta cttggttctt caggagttgg aaaatcttca ttaattaatt gtctgatggg
    17461 tggagaattt ttaaatacaa aggaaattcg catagatgat gataaaggaa gacatgccac
    17521 gactagacga gagctatttt taatttcagg tggtggtatt gttattgaca caccaggtat
    17581 gagagagttg ggaatgtggg atgtttcaga aggtttagat aaaagttttg cagatataga
    17641 agcttatttc ggaaaatgtc attttcgtaa ttgtactcac acctcagaac caggctgtgc
    17701 tatctgggaa gctattgctc atcaagaact ttcgcctaaa agatgggaat cctatcgtag
    17761 gcttaaaact gaagcagtat ttgcagacaa taaaagcgaa tacttagcaa aaaaagaaaa
    17821 gaaatttaaa aatatctcaa aaataaataa gaagattaga agataagtgt agtttttttg
    17881 ttcattctat gagtctatat actattaata tattaaattt gccatcaatt tgttacttta
    17941 atgtgatttt gatggcaaat ttggtttgtg gtattattta aagcaattta ttaatttttt
    18001 taataaagtt tgatttttta tatgatgata tatacatgtg ataatgtata catatttgtt
    18061 acgataggct cctttataaa gaggtcttat gtaagataag aaaaggcata tttatactaa
    18121 tacataatag agtatatgct tatgtatgat agagatgttt cttaagagca aaactgcaat
    18181 attgctaatt tataaagtaa aaatatatga attataaaat acaaataata tatttataaa
    18241 aattattgtt ttaatttata ataaaaattt tttgttgaaa ataatgctcg aacctataaa
    18301 actattaact aatatttgta aatgtatact tttgatgata aaatataatt aagttaagtt
    18361 ggtttttata aacataaatt tattttaagg aggtgaaata aatgaaaata tctagtcaat
    18421 atagaagtca atattcattt agatatgaaa gtaatataaa taatacaaga ataaatgaaa
    18481 gtatggttaa gaaaaatgaa actgtaggaa aagacactta tttatctaat attatgaaac
    18541 aaaagcaaga acttaatgat agaattagag atttaaaata tagacaagag gtttatacta
    18601 aaaaaataaa tgacgcaatt aagaacttat gtaaatcaga aataagagaa acaactaata
    18661 atttttctaa tatagaaata ggtattaaaa atagcattat agaagagaaa aataaaagta
    18721 caatgttaga tgaaaattca acttatctaa atacaaatga tgaaaaagaa tctttaatca
    18781 ctaaagagtc taatgaaaaa attgaagaag aaatattaaa tgatgaaaaa ttagaagagt
    18841 tagaacaaaa aaaggattat aaagaggatt ctaataaaaa agagaaagta tctgaggact
    18901 tatctttagt aggtaaaact cgtgaagagc ttgaaaatat gcttaaaaat tttataaatt
    18961 taacacaaga agaaataatg aaacttgagt cgagaataga aaagttagat aaaaatgctg
    19021 aagaatacaa acaaaattca aagactaata tatttgataa aacagatgaa caaaaaaaac
    19081 atataaatgt actgatttaa acaatatgtt ttaaaaaaat aagtttatta tataatgaaa
    19141 atataaaggt acaatttcaa tacacttcta ttaaaccaat aatttatgaa tcagatttaa
    19201 tttcaattta ctaataacca tatatttatg agttaataat tttatgtttt aaaaagaatt
    19261 aaataagggg gagtagtatg aagaagaata tattaactac tatgattacc atgattatta
    19321 tattgatgat gagtatgaca gtaatggcag agccatatca agaagatgaa acatctagct
    19381 tgaaaaaaca gagtgcaaat gaacgtaatc agactaatat aggcacaatg gatggcagag
    19441 tgcaaacaaa tgcaataagt aaaggtgcta attatgaccc taaatatcct cttaaaggaa
    19501 tgttaacaca acttggtctt aatacaacag gtaaagaggg atgggattta ctctatggag
    19561 gaaatcccaa tggagttgag cttcgagcag atggtaaata ttattatata aataagggta
    19621 tggaagggct aggattttat gcggcagcat tgtcaggtgg ctcatatcag agaataggct
    19681 ttggaaaact tgaccctata gaagtaatag ctgatggaaa ttggatttct tacaaacaag
    19741 ctcaagatac acttacagta atcaggaatt ttttaaatag ctttgactgg agaaatgcat
    19801 ctgagatgga aagagctaat agagctgcaa aattggtaac cgaagcaaaa tatgcagact
    19861 ctaaatattg taacatagtc tatggtaatc tggttgataa acgtggtgta tgtggaagtt
    19921 ttgcaagtag ctttcattta ttaacaaggt taatgggaat ggattcacta tctatattaa
    19981 atccatcatt aaatcatgct tggaactata tacaaattga tgggaaatgg tataggtttg
    20041 atgggtctga aatatcagct tttggtggat cattagactt tgattaccgt aaattaaagg
    20101 atgcaacaag agagatgact acttactatg atgctaaggc attgtctata ttaggattta
    20161 atcaataaaa atagggggag gcttcttata gagccttttt ttattgattt gaaatttaat
    20221 aaggaatttt atatgaaaat ttaatcaata aaagcaaaaa aggctcttta taaagccttt
    20281 ctaagtgttt ttttaagtaa aattttttat atcaacatca gttttaatca aaacatataa
    20341 cattttggac ttacttttaa aaataaaatt attaaaatat aatattaatc atgtttttag
    20401 tttattatat tctataatag tggtcatgtt gactattgta gttttattaa gaatagttgg
    20461 tttatataaa tatatgcaaa gatagcttgt tgtttacttt acttattagt atataggaca
    20521 agatactcta ataaatttgc ctattttaat atcttttgta ctaaaaccaa ctaaagtttc
    20581 ctttattatt tcgttttgaa taatacaaag cataatccga ttgtttgtat aaagactgga
    20641 aagtatcaga gttattttct aatttagata tacctataga acaagaatta ctctctaggc
    20701 ataattctaa aaccttcttt gccttatttt caacattctc ttttgattga ttactatatt
    20761 caaaaacagc aaattcatct ccaccaagtc ttccaataat agcatcattt acgaatactt
    20821 tagtcaaaat atttgaaaaa tgtattagta tttcatctcc tcgaacatga ccaaagatat
    20881 catttacctg tttaaaatta tctaaatcaa agatgtaaaa gtatccatgt gaaacagttt
    20941 taagcatttc ttttatgtgt acttcaaaaa aatgtctatt gtatagtcca gtcataaggt
    21001 ctttttctga ttttagttta aattcatttg ccattgctag tgcttcttgt gctttttcag
    21061 ttattgtttt agggtaatac tcatctttca tttggtcaaa gtttggaata gaatggtgaa
    21121 catggataag tttaatcata tcatctgtaa ctctgtatac catagtaaat cgagtatcca
    21181 tttctacata gatatctttt tctaggtaat ctttttctat tacccaaaga gtaccatata
    21241 ctataaaagc ttcgtctgta actttggtac atgaatacca ctcatctata atctcaaagt
    21301 ctatttgctc tgattctgtt tggtcttttt caagacctat aataaattct tctaatgttt
    21361 cgtaaaattc atgttttcct gttccaatta atgcaaggtt gtcatctaat aaggaaataa
    21421 taaatttaag gttttcttct tctggattta aaatataaat atgccaaatt ttttttgata
    21481 aatctcttaa ttgtttttgt attgtattat tttccataga ttaccccctt aagatttaca
    21541 gtttatattt taatgtatta taagttgaac tcttattaca atattattat aatatagaac
    21601 taaagaaatt acatcctgta taagtaaaag tatatctttt ataataaaaa tattacagta
    21661 aaataaccag ttttaatata cgtattatat ttttaattta taaacgaata ttataaatga
    21721 aatttaaatt caaccctgaa gaaataagct aaatgtatta gtatataaat ccatacttga
    21781 acataaataa cctttatgat atgatatcaa taataatatt atgagaggag tgccttgatg
    21841 tcggagttat ataatggtaa ccaacaatta aatagttgga gacacatata ttctgtataa
    21901 tcagaaattt caagtgtatt tttaatacat ttaatttttg tgtcttattc tgcctttata
    21961 gataactgac atatgacaac aaatagtatt taaaatatat attactttgt taaattatgg
    22021 tgtagtaggc tttaatctgc tacactttta ttaattttgt atagtattat tttgataact
    22081 attttgaaat agagagcatt aatttttcta gtccacagga gttttctgtt tttgtgctgt
    22141 taaatataag cacagagaaa ggcagatata tacctgtgga ctttttttat ataaattatg
    22201 aaatatggag gtagttaaaa tgagtataat tgaacttaat gaactgacta aatcttatag
    22261 aagatttgag aaaaaggcag gatttatggg gagtgtaaag tctttattta ataggaaata
    22321 tacagaaaaa acagctatat cgtcatttga tttaaagata gaagaaggag aatttgttgg
    22381 attaataggt caaaatggag ctggcaaaac tactcttatt aagatgttaa ctggtattat
    22441 tgcaccgtct tctggagaaa tatctgtaat gggttattac cctaacaaat tagaaaataa
    22501 ttttaaaaaa caatatgctg ttgttatggg ccaaaaaagt caactattct ttgaattgac
    22561 accagcagat acgtttcttt tatttaaaga attatatgat atatctaatg aagaatataa
    22621 gaaaaacttg gattacttta tcgaattatt tgaggtatct gactatttaa atgttcaagt
    22681 taggacgctt tctttaggag aaagaatgaa aatggaacta atagtagccc ttctacataa
    22741 tccaaaaata ttatttttgg atgaaccaac tataggctta gatgctatag cccaaaaaca
    22801 aattaggtat ttcctaaaag aagtaaatga aacaaaagga actactatta tacttacttc
    22861 tcattacatg gaagatatta agagtctctg tagtagaagt attgttgtag aaaatagccg
    22921 taaaatatat gatggttcta cagagaaact atttaattat tttcaaaaat ataaaaaaat
    22981 aacagttagt ttttccaatg aaactaacat tgtatttcca aaagattgta aagttgttga
    23041 acaaacaaat cataagatat cagtaattgt gccaaaagaa agaggaaatg aaacgattaa
    23101 atggattttt gataaatatg atgttaaaga tgtaatgatt gaagaagaag atataggtag
    23161 tattgtagag aggatatata agaaaggaag tgatgagtat tatgagcaac tataatgaag
    23221 caagttcaaa tgattcaatg ttttataaat ttaaacagag tattagaagg tattatgaag
    23281 tagccaaaat taactttaaa gtgcaactta tctggagatt tgatgtggtg gttaatatgg
    23341 tgtttacgat tgtaaaaatt ttatttgctt atattttatg gaaagcaatt tttggtgaaa
    23401 ataaaatagt ctctggtttt acatttaact ctatgttatc ttactatata ataagctcat
    23461 ttttagcagg tattgatatg tcaaagagta ccagtgaaga gatatgtaat cgtgtaaaat
    23521 tgggcacctt ttcaaagtac atgattattc caattaatgt tcaaaattat tttattgcta
    23581 aaaattttgg aatgacatgt ttttattttg tatttaatat gatttctgca tttgtatgga
    23641 tttttatatt taaaatagat tttgccttta catcaaatcc agttcaaata ttaatttcaa
    23701 ttttgatgat ttttctaggg tttattttta tgatgaaact ccacttcttt attgggattt
    23761 tgtcatttaa atttcttgag attagttttt taaaaatgat aattgaaaat attgttgaat
    23821 ttattacagg aacaatgata ccacttgtac tcttaccaga aactatagta acagttatga
    23881 aggtatttcc attttattat acaacatatt tgccatctat gttactaata ggtagaaaca
    23941 gcaatgaggc aatacaagga ttaattattc tagcattatg ggttttattt ttttctgtag
    24001 taaataaagt tatgtataaa agaatgagaa cattatttga tggggtgggt atatgatgaa
    24061 aaaaaatttg aaaataatat ttatgttatt aaaaatgaaa ttatcaaaga tgatggtatt
    24121 tagattcgat ttttttggag cattttttgt agatagttca ttatttatat tacaacttct
    24181 aatgtttaat agtatatatt ctcatgtaga ctcaataggg ggatggcaac aaggagagat
    24241 gttaatattt ataggaactt tctcattaat aaatgcgata aatatgacga ttttcttttt
    24301 tggtatttat gatattccac gaaaaatcca agaaggagaa ttagactact atattacaaa
    24361 accattagac ccactattta ggttaacatt tgaaaatatt aatattggtt catcaccatt
    24421 aattctagca agtatactta ttgttttata tggaatatct gagttaaact cagagataac
    24481 tttagttatg ataataatat atattttcat ggtgttattg atgacattac ttttttatga
    24541 tgtatgtatc atacttagaa cagtttcatt ttttgttata tcatcttctg cgataatgag
    24601 attagaagat aatttattgc caatgaatat gaaaattcct ggtgtaatat atgaaggtat
    24661 ttttaaagta ttattctatt taatcttacc atatggaatt atgtcgacaa ttccgactca
    24721 aattttggct gggacaataa caattaatgg attaatatac tcaattttaa ttgtattttt
    24781 gtttacattg tttatgtgga gattttggaa gttaggtatg agacattata aaagtgcaag
    24841 tagttaataa attagtaaaa atagacaatg tataactata tataaatacg tttcatatga
    24901 cactaaacta tgtgaacaag tatttgcgct aaaggcactg aaagctataa aatgattaaa
    24961 gtaaggattt aaaagtttct ttagtagaaa tataattgtg taaaaaagca agattaatta
    25021 aaactatatc caaataatta gatataattc ttatgcagaa aggaggtctg taaatgtcta
    25081 attgtttgga atatatttta aaaataatta atgtacagaa aaataaacaa aatgagtgat
    25141 gtatattatt cagaaaatca tgaacaagat aatctttatg agcaagataa tttactggca
    25201 caaagattta atttatttac tctattgaag tttgcatttc ctactatttt tatgatgatt
    25261 tttatggggt tgtatacaat cgtagatact atatttgtat caagatttat taatacaaat
    25321 gctctatcag caattaatat tgtatgtcca attattaatc ttatagttgg aattggaact
    25381 atgcttgcca ctggaggaag tgctattgta gcacgtaaga tgggaaatgg tgatattgag
    25441 ggagcaaata aagctttttc tttaatcgta ttatcaggag ttgtaattgg ggtaataatt
    25501 actggtatag gtattgtatt tatggattat atcattatat ggttaggagc aagtgaagtt
    25561 ttaattaaat attgtcgttc atatcttttt attatcttgg tttttgcttc tgcaaatata
    25621 ctacaagtat tatttcaatg tttatttgta acagctggaa agcctcaatt tggattatgg
    25681 ctagtgttag gtgctgggtg tgctaatata attcttgact atgtgttcat tgttctgtta
    25741 caaatgggta ttagtggagc agcacttgca actggagtag gatacctaat tcctaccatt
    25801 tcaggtatgg ttttcttttc aaaaaataaa gggttactta aattttgtat acctagacta
    25861 gataaaagaa tattatttga agcttgtttt aatggttcat ctgaaatggt gggtcaacta
    25921 tcaacagcag ttactacttt tttatttaat gctatgatga tgaaattaat tggtgaagat
    25981 ggggttgcag ctattacaat aattatttat tctcaatttc ttttaacttc gctttatata
    26041 ggtttctcaa tgggtgttgc accaataata agttataact atggaagtga aaataatacg
    26101 cagttaaatc atgtttttaa gatttgtata ttatttattt cagtagcatc tttattggta
    26161 tttatctttt ctatatcatg ttcaccaatc attgtagaga ttttttctca aagaggaagc
    26221 cgagtttatg aaattgcaag gtccggattt tatatatttt cttttagttt tattttttgt
    26281 ggatataata tttttacctc atctatgttt acagcattat caaatggaaa ggtgtctgca
    26341 atattatctt ttctacgtac atttggattt ataactatag gtttattgat attgccaaat
    26401 atattaaata taaatgggat atggttagca gtaccaagtg ctgagatttt aaccttattt
    26461 ttaactgttt tttgtatatt taagtataag aatagatata aatatatgta gatattaaat
    26521 aaatagctta gatagatatt ttatgtttta ataagtaatt caattagtgg tagatatata
    26581 ctattgaaaa ttaaagttat aattgatata aagaattaat aaaaataaca tataaaagtt
    26641 taaaagggag ttgaacttaa agtttgatta gaaaaagtca attttaagac aactcctttt
    26701 attttactat tgtattaatc tgtatctgta agtgtattag tctaatatta taaactttgg
    26761 atgaaattaa ttttctatag gttttaatgt atcatacact ttattttaaa gaaacttatc
    26821 ttaattttca tagtgattga atttgtctat tagagatgtt actattagta atgtaaaagc
    26881 aagagtagtg aatataaaaa acaagatagt gatactgcaa gattagaata taataaattt
    26941 ttatggagag atttcaatat ggatacatgt tttaataaaa aaatattact tgttgatgat
    27001 gaaaaggata tagtagattt aattgaagaa gtactcataa atgatggttt taaaaacatt
    27061 attaaagctt ataatggtct agatgctatt tcgctgtgta agatagcatg tccagatgtt
    27121 gtcattcttg atataatgtt accagatata gatggtatag aagtgtgtaa aaaaattcga
    27181 gagttttcat actgttctat tcttttttta tcatcaaaaa atgatgatat tgacaaaata
    27241 ctaggtctta gtagtggggg agatgactat ataacaaagc cattcagtcc aagagaaatt
    27301 gcttttcgag ttaaagcgca attacgtaga caacagtatc aaagcattgt accatcagat
    27361 agtgaagtta ttaaaatagg agatattact attgatatag aaggaaaccg tgtatataaa
    27421 gatagaaatg agattgagtt aactggaagg gagtatcatc ttttaagcta catggctcaa
    27481 aatgtaaata aaattatagg aaaagaaaga ctctatgaac aagtatgggg agtatatagt
    27541 agtatttgtg acaatacaat tatggtgcat atacgacata taagagaaaa aattgaagat
    27601 aacccctcaa atcctaaaat acttataaca gttaaaggac taggatataa acttgtgaat
    27661 agaatcgatt agaatagatt ttaatattaa tgaagatatc tcaagttgaa tttttagtta
    27721 atgaggcatt cttttatggg tgaaatcaaa tgtattttcg ttatctcaac aatgaagaag
    27781 aggtctatct ctattttgag atagcctcat taagtttata tcctggtaat gtactttatg
    27841 ttctaataaa atttttttag tttatttgca gtgaaacatt aactagaaca ttaaaatttg
    27901 cattgaaata ccaattaaat aatattgtat attgtgtata aacttatact aattgttttt
    27961 ctatattaaa tactttatca atccaacctt tataaaattt ttcttcatgg gaaactagga
    28021 ttatacttcc tctaaactgt ttcaaagcat tttgtaaagc atcctttgtc tctgcatcaa
    28081 agtgattggt tggttcatcc agaataagaa aattacaagg agataacatc atacaacata
    28141 atttaacctt agattgttca ccaccactta aagtagatac actccttgaa acatgctctt
    28201 ccttcacacc acatctggct agatgacgtc taatctcttt tgtatttaat tttggatatt
    28261 tatctgctac tatttgaagt ggggttttat caggattttc ccattttaaa tcttgctcat
    28321 aataaccaat tttaacttgt tctgaaaact ggaaatctcc agatatacga ggaatatctt
    28381 tgactaatgt ctttaacaag gtagatttac cgattccatt aaaaccagtt ataactattt
    28441 tttgacctcc atcaacagag aaatttaatt ttggaagtaa agaatagtag tatccaactt
    28501 caagatttgt tatagtaaga gcattttgag ccgatatttc aatttctgaa aattgaatat
    28561 ttggttttcc tgtaaaacta ggaggagcta ttctttctat tttatccaat tgtttttgtc
    28621 ttcctcttgc aatttttgaa tttactccag ctttattttt acgtataaat gtttcttctt
    28681 tttctatttt cttttgttgt gcttgatacc ttctaatata atcttctctt aaatgtgatt
    28741 tttgtttaag gaactctgaa tattttccat gatatttctt aatcatgcca aattctatat
    28801 caagaatacc tgtagaaatt ttttctaaaa agtcaaaatc gtgggaaact acaataaatg
    28861 ccccattaaa tgtatttaaa taatttgaga gccattcaac atgctcttta tctaaaaagt
    28921 ttgttggttc gtctaataat agaacatcat ggttagaaag tagtaatttt gctaaaatta
    28981 cctttgccct ttgaccacca ctaagctctc taacaacacg attcattcct attgaatcta
    29041 atccaagacc atttgctatt ttatttattt cattatctat agaatagaaa ttacttgcta
    29101 ttagttgttc ctgataatct gatgctttga ttagatattg ctcattttca gatatagcac
    29161 ttttttgata gagtaaattc atttcctttt ctattttata aagttcttca aatgcagtgt
    29221 gtaaatagag tgatattgtt gtatctctat tgatttctgc atattggtct aaatggccta
    29281 tattgatatt aggttgccac ttgatttctc ctgaatctgg aacaacttct cccaataaaa
    29341 ttttaattaa tgtactttta cctgtaccat tttggccaac tacacctata tgttcacctt
    29401 tgaataaatc aaagttagct ttctcataca gtgatttatc cataaaacat tgtgataaat
    29461 tagttacttt taataaactc ataattcaaa ctccttttct tttattaaaa aatttatgga
    29521 aataaaaaaa gcagaggaat ggcattgtaa aatgccgacc tctgcttttg gaaaaacaaa
    29581 aggtcataga caagcattgt tgtctatgac cagaaaatct atattatcta tcggcaaata
    29641 ggtaatttta aataagcata gccaaaaaac tatactaaac ctatatttgc cgatgaagat
    29701 agaaagcttt aagctttcag attaacctct acacaacgct tcatcgtata catttgtgta
    29761 gagttaagtt ttctaacaag tttaactaat tctatataaa tcataattat tcacatccta
    29821 atcttagtat taattctagt ttatctgtat ttaaattgta cttttgtact ttgtggatta
    29881 tcttatttat ttaaaatatg ttttattaat tatagcttaa gttatgttat cataatactt
    29941 agttatagtc aataagaaag ttatatttac tattattata ttaatttagt aaatctatca
    30001 ttacaaagtt atttgaagac tcgctatcaa taaaatttta tagtctataa gaaaattaag
    30061 attatgagtt ttataatttt gatataggga tactattttc ataaatatta caacatgtag
    30121 aagtatatgt ctattattgt tgaattttca atgatatatt aaggttctaa tatattatac
    30181 aaaataaaaa cattaattca acttaaataa ttttaagtat tatatgtgtt ttgattaaag
    30241 aaaactaaaa ttttaaggaa aaataacata tttgtattaa attttattga aatgattaaa
    30301 tttatttact taatgtaaat tattagctaa aattgtatta aataattaag gaggtgagta
    30361 gctctggatg aaatagattt aaaaataatt aattctctaa aaaagaatag tagggcttca
    30421 acctctgaaa taagcagaca aattaattta tcagtaccag cagtctcaga gcgtataagg
    30481 aaaattgagc agtcaaattt aattcaaaag tacactattc aaattaatcg ggaaaaaaca
    30541 aaatataagc ttttggcttt tatatttatc aatactgatt tatctaaaag tatgaaagaa
    30601 tttaagaaaa ctatattgga atatgatact gtattagaat gtcatcatat ttcaggagag
    30661 tatgattact taataaaagt attagcagaa gatacaagta atcttgaaga gtttatatca
    30721 gtgactttga aggaaattgg ggggattcaa aaagctaata caaccattgt tttatctaca
    30781 attaaagaag aatttaatac aataaaagat attttttaaa taatttacta gtaaagtgtt
    30841 aattatattt taggaggaaa atagaatatg aaagttttag tttttttagc aaaaggtttt
    30901 gaaactatgg aatttagtgt atttgtagat gttatgggat gggctcgtaa cgattatggg
    30961 catgatattg atgtagtgac ttgtggattt aaaaaacaag ttatgagcac atttaatata
    31021 caagtattag ttgataagac gatagaagaa gtatgtgttg atgattatga tgctctagct
    31081 attcctggag gatttgaaga atttggattt tatgatgaag catatgattc atctttttta
    31141 aatctaataa gggagtttaa ttcaaaagaa aaaataatcg cttctatttg tgtggcagca
    31201 ttgccagttg gaaaaagtgg tgttttaaaa aatcgtaaag ctactacata tcatttgaaa
    31261 aatggtaaaa gacaaagaca gttaagtgag tttgatgtaa atgtagttaa tgaaccaatt
    31321 gttgtagata aaaatattat aacctcatat tgtccagaaa cagctccaca tgtagctttt
    31381 aagcttttag aaatgttgac atctaaagaa caaatggatg aagttaaatt agcaatggga
    31441 tttaaactgt aatggtttat agattttcta agtaatcaga accccagtca cacatggaat
    31501 ctaaaattgg aataatactc tttccaaaat cagatagcga gtattctact tttggaggaa
    31561 caacaggata aaccactctt ataattaaac catctgattc taattcacgt aattgttgag
    31621 ttaacatctt aggcgttgct ttaggaatta atttttttaa ttcattgaac ctcaatatag
    31681 tgtctttaag atgccaaagt ataagtgtct tatatttacc accaatcaat gcaagtgttg
    31741 cttctattgg gcaactgtaa ttatctaaac attttttcat ttttgggctc ctttatttat
    31801 tttaaagtat ctttttaggt actatattac aaaaaagtgc attattgtaa tatagtacca
    31861 tagtgataaa attatagcat aggttaaata aatcatcaac ttagcctaat aaaacaatag
    31921 ggggtaaata aatgaatttc gttgaacttg caaaaaaaag atattcttgc cgtaattatc
    31981 aagatagaaa ggttgaaaaa gaaaaacttg agaaggtttt agatgttgca agaatagctc
    32041 caactggtgg taatcgtcaa cctcaaagat tgattgttat tcaggaaaaa gaaggtataa
    32101 ataaactttc taaggctgcc aatatatatg atgcgccatt agcaattctt gtatgtggag
    32161 ataaagacaa agtttggaca agaccttttg atgggaaaca acttacagat attgacacaa
    32221 gtatagttac agaccatatg atgttacagg caactgaact tggattggca agtgtttggg
    32281 tgtgctattt taatccagat ataattagag aagagtttag tttgccagat aatctagaac
    32341 caataaatat attattgatg ggatatgaat ctaagatacc agagagccca gagagacatg
    32401 aaaaaactcg tgttccttta agtgaaattg tttcttatga aactctttaa ttataaaatt
    32461 aagttattca taggataaat gtaataaagt gtttgttata aacaaaattc aaaataaata
    32521 atatctacat ataattaatt ttagtgaagt atatctagtt taactagaat gtatacttca
    32581 ctaatttatt tattcaaaat atttatgagg attttaatat attaactaaa atatatcaat
    32641 tgaattaact tttaaatctg gaaaagcact tgaaatatag tgttttatat catttaaatg
    32701 gtcttgaatt tgcttttctg atattttatc aggtccatat acaaaataca taatattttt
    32761 agtatctttt gttatagctg ttcgattatc agggccatta agaatactag ataatatacc
    32821 aatattatct gataaaaata aatcatcttt tgttaaaata tgttctttac cacctatacc
    32881 ataaaaatgt tcacttccat ttgctaattc tattttcaaa ggtaattcca ttttatcaag
    32941 gtcatgacct gctgttagaa gaagattttt caattctgct aaaaacattg actcaattgt
    33001 tacacctaca tctgggatac cttttgattt taatagaata gattctaatt gaagcaatac
    33061 agggtaagtt ttcttgaatt ttttataata ctttatataa cttgaaacag gctctgtttt
    33121 aattttttct ttgcgattgt attctgggtg ttgttctata atatttttaa ttgtagagtt
    33181 tttaagaact aaaaaatttt ctttattagg ttttgaatgt gtaatattta taaccataac
    33241 tccaaattta atattgggat aaatttcttt aactttatgt gatacctcta acataatata
    33301 gactcctttt tattttatat tttatgatat aatcttagca aattaaaagt ggattttata
    33361 gttccatttt tatagtaatt ttatagaacc acttttggag gtattagaat gtggattacg
    33421 attaatagaa atagtaatat atcattatca agacaaattt attgtgagat aaaagatatg
    33481 attctggatg gaagcttaaa gtttgagcaa aaactgccat catctaggac tttatcaaaa
    33541 gaactatctg tatctcgtaa tacagtttta gatgcatata atcaattaat tgcagaagga
    33601 tatttggaaa caaggaatgg atatggaaca attgtagcta aaggaatatc agggcataaa
    33661 gttgatttta agttaaatga tacttttaga gatgaaaaag atttacatac taataaacaa
    33721 aaaaattata tagattttcg ttcaggagtt ccagaattaa aactatttcc acgcaaagaa
    33781 ctttcaaagc tatataaaca aatactgtat gatttatcag attcggattt cagatatgga
    33841 agtacagctg gtgtatgggt tttaagagtg gaaatatcta agtatttatt tcgtactaga
    33901 ggaattagat gtaatcccaa aaacattatg attgtttctg gttcaacaca aggtttatct
    33961 ttaatttcac agcttttata caaaagtaat aaaaaggttg ctgtagaaga ccctattcat
    34021 aaaggcttat taaaggttat ttcatctatt ggatattcag ttgttggaat taaagtagat
    34081 gataagggaa tgaataccaa cttaataaaa tcaataaagg ggatttcttt tgtttatact
    34141 acaccttctc atcaataccc attaggaggg attttaccta ttcaaagaag actagagctt
    34201 attaaatgtg caacagagaa tgattactat ataattgaag atgattatga tagtgaattt
    34261 agatatgagg gtcaaccaat tagttctctt tatgaactta gcccaaataa agtaatttac
    34321 ataggttctt ttagtaaaat attatctcca gctattcgtg ttggatttat gttattacca
    34381 gacaatattt tagaaagcta taaaacctta aaaatgtact ctgatgttca tacagaaaca
    34441 atattgcaac atgtattagc tgaatttatt ggaaatggag gtttagagaa gtacatattt
    34501 agaatgaaga agttgtacaa tagaaaacgt aagtacctca taaaagtttt aaacacggaa
    34561 tttaaaaatg aatttaaaat aaaaggtgaa gcagcagggc ttcatatagt tgctcatttc
    34621 tataatgtta aatttacgga aagtttaatt cataaaattt ttgaagcagg tataaaagtt
    34681 tatcctatac aacaatatgc aattgagaat atattagaat atgactatga aattatacta
    34741 ggttatgctc atcttagttt tgatgaaatt gaaaaaggtg taaaaatact cagcaaaata
    34801 atacatatga atcaaggtac atagataaaa catgtagcaa tctacatata atagatgtaa
    34861 ttaagtttaa actttaaaag aaatatatat taatattttt gtataaaaat acctgatatt
    34921 tttttatcga tactttattg ctatacacta aattaagata gcaatttata ttaagaaaaa
    34981 actttttgca taggttataa tacttaaaag gagaggatag agttgaaaac aaatacagat
    35041 gttattatgg agataattga ttatatagaa gaaaatctta cagaaaatct aaatttagat
    35101 aaaatagctt atattgctaa ttattcaaaa taccatttac acagaatgtt tacaaatgtt
    35161 gtaggatgta ctatccatca atacatacag aggaggagac ttactgaagc agctagacaa
    35221 cttatattta cagacaaata tattatagac atagctatta cagtaggata ttctacacat
    35281 caatctttta cactagcatt tagaaattta tataaagaat ctccacaagt atatagaaat
    35341 aaagatgaat tttatcctat tcaacagaga tttgttttaa atctaaatca tcaaattcaa
    35401 agtgaaaaat gtatttctta tgcaaatatg aattataaac aaagaagaga tgaaatcaat
    35461 tcttatctta actttaatat taattgtgag gtgaaagtag catgagttta gtgccacatg
    35521 taattgaaca aacaggtcaa ggggaaaggt cttatgatat atattcaaaa ttattaaaag
    35581 atagaattat atttatagga gaagaaatta atgatgctat tgcaagttta gtagtagctc
    35641 aattactgtt tttagaatca gaagacccag attcagatat aattatatat ataaatagcc
    35701 ctggtggctc tgctacatca ggatttgcta tatatgatac tatgaattat gtcagatgtg
    35761 atgtatcgac tatttgtatt ggtatggctg caagcatgag tgctttttta cttgcaggag
    35821 gaacacatgg aaaaagatgt gctttaccta attcagagat tatgattcat cagccaatgg
    35881 gaggagctaa aggtcaagca acagatgtaa aaatagctgt agataatata ttgaaaataa
    35941 aagaacgatt agataaaatt ctttctgaaa atacaggtaa atcaatagaa gatattagga
    36001 gagatacaga tagagataat tttatgactg cactagaggc taaagagtac gggctgattg
    36061 attatataat gaataggaat gaataaagta tcaaagtatc atattaaaat gtctgtttta
    36121 caaatatata tataatatta aataagagtg tctcaaaatg aactttttag tttatgggac
    36181 actctttttt gtatgaaatt aaatatggtt ttatcatttc aacaattatt accaaatttc
    36241 aagtttaata aattctacta taaaaaatac ataaaagata aggttcttta gtaactttct
    36301 gtcgtcaaaa gtttttataa atttaataac aaaacaagag aaaataccat atattactta
    36361 tgtctgcaat atatagaaga caaaggagtt gtacattatg attacaataa gtttgtgcat
    36421 gattgttaag aatgaggaag aagttatagg cagatgctta gaatgtgtaa aggatatagt
    36481 agacgaaatt ataattgtag atacaggttc tacagattcc acaaaagaaa tagtctcaaa
    36541 atatacagac atggtgtacg atttcgaatg gatagatgat ttctctgctg caagaaattt
    36601 ttcattttct aaagcttcta aagactatac tatgtggctt gatgcagatg acataatttt
    36661 agaagctgat agagaaaaat tattaaagat taaagaaagc ttggatactt ccattgatat
    36721 tgttatggca aaatacaatg tatcctttga tgaaaatggt aacccaactc tctcgtatta
    36781 tagagaacga ttgtttaaaa gaagttctaa tcataaatgg gttgacccta ttcatgaagt
    36841 agtacctctt tttggagagg tattttattc tgacattgca atatctcata aaaagcttca
    36901 tagacaggac cctctaagaa atcttagaat ttttgaaaaa atgatttcag aaggaaaaac
    36961 attggagcca agacaccaat tctattattc tagagagttg tactataatg ctaggtataa
    37021 agaagcaata gaaggtttta ctaagttttt agactcatca agaggatgga ttgaggattg
    37081 tatcagtgct tgcagagatt tagccacatg ttattactta attaatgatg aaaaaagtgc
    37141 cttatattca ttgtttagaa gtttcgaatt tgatgagcca agagcagaaa tttgttgtga
    37201 cataggaaag catatgtttg atagacaaaa atataaagaa gccatttttt ggtataaggt
    37261 agcattaaca agagataaga atgatactaa tggaggtttt aagtcaaatg attgttatgg
    37321 atatatacca tctatacaat taagtgtctg ttatgacagg ttaggagaat cagataaagc
    37381 tatttattat catgaaaaaa caaaagaaat caaacctaac gatagtgctg ttttgcacaa
    37441 tgaaaattat tttagtaaat ttaaaaatag ttgataggtc acaatcatac taaatttgac
    37501 atattatata tagagcttaa aagctcaaat atatcaggag gtgcttttaa taatgagtag
    37561 aaataaatat tttggaccat ttgatgataa tgattacaac aatggctatg ataaatatga
    37621 tgattgtaac aatggtcgtg atgattataa tagctgtgat tgccatcatt gctgtccacc
    37681 atcatgtgta ggtccaacag gcccaatggg tccaagaggt agaaccggcc caacaggtcc
    37741 aacgggtcca acaggtccag gagtaggggc aacaggccca acaggaccaa ccggtccgac
    37801 tggtccaaca ggaaatacag ggaatacagg agcaacagga ttaagaggtc caacaggagc
    37861 aacaggggca acaggcccaa caggagcgac aggagctata gggtttggag taacaggtcc
    37921 aacaggccca acaggagcga caggagcaac aggagcagat ggagtaacag gtccaacagg
    37981 tccaacggga gcaacaggag cagatggaat aacaggtcca acaggagcaa caggggcaac
    38041 aggatttgga gtaacaggtc caacaggccc aacaggagca acaggagtag gagtaacagg
    38101 agcaacagga ttaataggtc caacaggagc gacaggaaca cctggagcaa caggtccaac
    38161 aggggcaata ggagcaacag gaataggaat aacaggtcca acaggagcaa caggagcaac
    38221 aggggcagat ggagcaacag gagtaacagg cccaacaggc ccaacagggg caacaggagc
    38281 agatggagta acaggcccaa caggagcaac aggagcaaca ggaataggaa taacaggccc
    38341 aacaggtcca acaggagcaa caggaatagg gataacaggg gcaacgggat taataggtcc
    38401 aacaggggca acaggaacac ctggagcaac aggtccaaca ggagcaacag gcccaacagg
    38461 agtaggaata acaggagcaa caggggcaac aggagcgaca ggtccaacag gagcagatgg
    38521 agcaacaggt ccaacaggag caacaggaaa tacaggagca gatggagtag caggtccaac
    38581 aggagcaaca ggagcaacag gaaatacagg agcagatgga gcaacaggt
//
LOCUS       DAMJWU010000028        37577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMJWU010000028 DAMJWU010000000
VERSION     DAMJWU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.028"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..471
                     /locus_tag="PC460_001585"
     CDS             <1..471
                     /locus_tag="PC460_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2600473.1"
                     /translation="QRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLI
                     KENDIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFAS
                     SQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            903..1157
                     /locus_tag="PC460_001586"
     CDS             903..1157
                     /locus_tag="PC460_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600474.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1193..1516
                     /locus_tag="PC460_001587"
     CDS             1193..1516
                     /locus_tag="PC460_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600475.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIF
                     IDYII"
     gene            1790..1942
                     /locus_tag="PC460_001588"
     CDS             1790..1942
                     /locus_tag="PC460_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600476.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2136..3059
                     /locus_tag="PC460_001589"
     CDS             2136..3059
                     /locus_tag="PC460_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2600477.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3326..4006
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3326..3353
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaa"
     gene            4198..4350
                     /locus_tag="PC460_001590"
     CDS             4198..4350
                     /locus_tag="PC460_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600478.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4470..5284
                     /locus_tag="PC460_001591"
                     /pseudo
     CDS             4470..5284
                     /locus_tag="PC460_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5336..5497
                     /locus_tag="PC460_001592"
     CDS             5336..5497
                     /locus_tag="PC460_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600479.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5826..6290)
                     /locus_tag="PC460_001593"
     CDS             complement(5826..6290)
                     /locus_tag="PC460_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600480.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(6426..6743)
                     /locus_tag="PC460_001594"
     CDS             complement(6426..6743)
                     /locus_tag="PC460_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600481.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7026..7322)
                     /locus_tag="PC460_001595"
     CDS             complement(7026..7322)
                     /locus_tag="PC460_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600482.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITNEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(7341..7754)
                     /locus_tag="PC460_001596"
     CDS             complement(7341..7754)
                     /locus_tag="PC460_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600483.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7738..7971)
                     /locus_tag="PC460_001597"
     CDS             complement(7738..7971)
                     /locus_tag="PC460_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600484.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8190..8564
                     /locus_tag="PC460_001598"
     CDS             8190..8564
                     /locus_tag="PC460_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2600485.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            9176..9868
                     /locus_tag="PC460_001599"
     CDS             9176..9868
                     /locus_tag="PC460_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600486.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            10064..12124
                     /locus_tag="PC460_001600"
     CDS             10064..12124
                     /locus_tag="PC460_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2600487.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(12330..12653)
                     /locus_tag="PC460_001601"
     CDS             complement(12330..12653)
                     /locus_tag="PC460_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF2600488.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(12646..13347)
                     /locus_tag="PC460_001602"
     CDS             complement(12646..13347)
                     /locus_tag="PC460_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF2600489.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(13625..15010)
                     /locus_tag="PC460_001603"
     CDS             complement(13625..15010)
                     /locus_tag="PC460_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2600490.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALVEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(15456..15878)
                     /locus_tag="PC460_001604"
     CDS             complement(15456..15878)
                     /locus_tag="PC460_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF2600491.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            16356..19259
                     /locus_tag="PC460_001605"
     CDS             16356..19259
                     /locus_tag="PC460_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2600492.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISVSEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            19292..19738
                     /locus_tag="PC460_001606"
     CDS             19292..19738
                     /locus_tag="PC460_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2600493.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            19861..20163
                     /locus_tag="PC460_001607"
     CDS             19861..20163
                     /locus_tag="PC460_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2600494.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            20344..21750
                     /locus_tag="PC460_001608"
     CDS             20344..21750
                     /locus_tag="PC460_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2600495.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            22094..23152
                     /locus_tag="PC460_001609"
     CDS             22094..23152
                     /locus_tag="PC460_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2600496.1"
                     /translation="MGDKMRASVLYSVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            23251..23937
                     /gene="araD"
                     /locus_tag="PC460_001610"
     CDS             23251..23937
                     /gene="araD"
                     /locus_tag="PC460_001610"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF2600497.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            23955..24602
                     /locus_tag="PC460_001611"
     CDS             23955..24602
                     /locus_tag="PC460_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2600498.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(24775..26142)
                     /locus_tag="PC460_001612"
     CDS             complement(24775..26142)
                     /locus_tag="PC460_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF2600499.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(26332..26420)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            26848..28968
                     /locus_tag="PC460_001613"
     CDS             26848..28968
                     /locus_tag="PC460_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF2600500.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKTIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKTCNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNILEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            29072..30325
                     /locus_tag="PC460_001614"
     CDS             29072..30325
                     /locus_tag="PC460_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2600501.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSKMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(30478..31539)
                     /gene="ytvI"
                     /locus_tag="PC460_001615"
     CDS             complement(30478..31539)
                     /gene="ytvI"
                     /locus_tag="PC460_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2600502.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            31813..32553
                     /locus_tag="PC460_001616"
     CDS             31813..32553
                     /locus_tag="PC460_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF2600503.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            32565..34343
                     /locus_tag="PC460_001617"
     CDS             32565..34343
                     /locus_tag="PC460_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2600504.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            34510..35427
                     /gene="pfkB"
                     /locus_tag="PC460_001618"
     CDS             34510..35427
                     /gene="pfkB"
                     /locus_tag="PC460_001618"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2600505.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            35464..37383
                     /locus_tag="PC460_001619"
     CDS             35464..37383
                     /locus_tag="PC460_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2600506.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT        14995 a         4063 c         6193 g        12326 t
ORIGIN      
        1 caaagagaat tatcaagaaa aacaatcggt agtttaaata gaaataaagc aagattaaaa
       61 gttgcaagac ttcaagaaca tatagctaat caaagaaatg attttctaca aaaactatct
      121 actaaattaa taaaagagaa cgatattatt tgcatagaag atttacaagt aaagaatatg
      181 attaggaatc gtaaactatc tcgctcaatt tctgatgtgt catggtcaga gtttattcgt
      241 caattagaat acaaggctaa ttggcatggt agacaaattg taaaggtggg taaattcttt
      301 gcaagttcac aaatatgcaa taaatgtggg tataaaaacg aggaagtgaa ggacttaaat
      361 ataagagaat ggatttatcc tagttgtaat gaaactcacg atagagatat aaatgcaagt
      421 ataaatatat taaaagaagg attaagacta ataacaattc aaaataaata acgtatgtaa
      481 gaaccgtagg aactacgggg atagcctgtg gagaattagt aagacatatg ttagtatgca
      541 aaattctgtg aagcaggaaa cctgtgactt tagtcatggg aggttcaggc tagtattact
      601 cattattgta ttcaaaagct aaagtggaaa cctagtgaat atatgaattt agaagttaat
      661 gagagagcat tagcagccga ctcaatactt attaaaattg aagatgaaga agaaacaatg
      721 aaagaagctg aaagagagaa aaagagaggt agaagaaggt agaatttaaa gttaaaatag
      781 ttacattgct aatatatgaa aatgttacaa atttggaaat agatggatat atatgttgta
      841 tagtataatg tagataatat atcaccaagt aaaaaacaac aaataaatct aaattatgag
      901 gggtggaaaa taaagatatt aaacttacaa tatgtatatt tgcttttgta attggaataa
      961 tagtgttatt ttgctgtata ggtctaggtc aatttaatat ggattgtatt ataaaacaaa
     1021 atggtgggtc aatagctacc gaacaataca acatatattt agaacaatct atttcacaat
     1081 atagaaattt tggttctata ctggttttac taggaggaat aggaatactg tttgataaaa
     1141 gtgtcagaaa atactaaaaa agtatatttt tatttaggat gtgatgaatg atatggcaag
     1201 agatgtattt agtgtaaaga tgacaccact agaagcacag aaaatggtag aaaaaaattt
     1261 ggaagcagat ttagtttctg ttgatattta taacctagaa aataataagt ctataatagt
     1321 tacaatcttt gaaaaatatt atgcaagaag caatagtgat gcaggtttgg tagtgatttg
     1381 tgaaaatact acaggaaaaa cacttgttaa aattacctca actggtagtg caattggact
     1441 atttcagatt gattggggaa caggtaataa tttgattaaa agagtaaaaa aaatatttat
     1501 tgattatatt atttaattat tttatgtaga tattcttagg ttggtatgga atttgtacta
     1561 acctattaag taatcaataa aactaaataa acatagaaag cacttacaaa tatgtaggtg
     1621 ctttttattg aaaaaataga aaataaaata aaaagtaaga tataggaaaa atatgtaaga
     1681 attatatgtt ataataattg tagcaagaag atgtaatcta caatttatag agtggagttc
     1741 atgtattaaa aaattatcct cccaacgata agaagggagg tgaatataca tggataattt
     1801 tttatttaat gtgttagcta gtgtaacagc tagttacata gtttatttga ttagcaagtt
     1861 aatcaaaaaa gtaaaaagcc actctaccgc aaagagtgac ttaaagattg atataaattt
     1921 taaatatcat cgtaaaaaat aactttcagt tatagaactt cactctaccg caaatagatt
     1981 gtagttcttc ttgtttttat tataccacaa attagaaaaa atattgcata taatattttt
     2041 tatagtcaat aaaaatatga aattttttat acacaatgat gatgaatcta actagatgaa
     2101 taaatttaat tgtaaataag aaagtactta cttaaatggt aggtgctttt gttttgccaa
     2161 attatgctat aatattgagt gagatgagta atttgtgggg gaattttata atgtggatat
     2221 tagtaccaat aataacaata gtattactta taatagctgt ttcttctatg caatatattt
     2281 tagttatgat agcttttttg ttgattatat attcttttat agaaaagaaa atagttatgg
     2341 gtcttgtgtc agttcttttt ttcacttatt caatttatct ttgtgcaact tgggaagata
     2401 aatccttgat agcagataat aaagttgaga ctgttaaggc acaaagagaa actgtagaaa
     2461 gagaaaaaga aatggaaaga agaagaatac aagaagaagt agacaaagaa agatatattg
     2521 aaaaacatgg aatggaaata tcagaaaatg atttaaaagt taagctagag gctttagtac
     2581 ctcaagaata taaggggaag aaatatgaat taaaagtagg aaaatttaaa agatatagta
     2641 tgtattttga tttaactgta caaaatgaaa aatttagtaa ttctgaagaa tgtaaaaaat
     2701 ttgtaaaaga aattgctaat gatcttaaaa aaattaaaat aagcaaggcg tactttaagt
     2761 ttcattcaaa agatgacggc ggtatatata attctgttta tattgattat tttagaaaca
     2821 tacaaaataa tgtagataat gttgaaaatt tagaattcaa tgagtttgaa cttaaaaccg
     2881 aagaagaaga gaagcgagaa caagagaaaa tagaacaaga aaaaaacagt tataataact
     2941 acattcaaaa cagagtggtt gacccactag atagaataaa gaaactaaaa gagttgctag
     3001 attcaggagc aattacacag gaagaatata ataaaaagaa aaaagaatta ttagaataga
     3061 taatatagta agcacttaca agtatgtagg tgcttttaaa tttacaaagt attccatttt
     3121 aattttatag tttagatttt atgatataat aaaaatatag aagttttgca gtgtgcgata
     3181 tttgttacaa agtagggctt aatacttgaa atctaagatg ttgagggtgc gtgataagtg
     3241 ttatcaattg cactattgcc cgctcactgc aattttaaga gtattgtata tatgtaggta
     3301 ttggaaatgc taagtttatt ttggggtttt agattaacta tatggaatgt aaaactgact
     3361 cgggagcata taataagtaa tatcaagaat agttttagat taactatatg gaatgtaaaa
     3421 ttcgtaaaat aacaattgtc aggaatatga gaagtttgtt ttagattaac tatatggaat
     3481 gtaaaattgg actcaaaata taagttaaaa aaatttttaa gttttagatt aactatatgg
     3541 aatgtaaaaa tttttgacgc tatgaagaaa tttactacaa aatttgtttt agattaacta
     3601 tatggaatgt aaaaactttt ttcatcctcc caaacctctt gttcgctgtc gttttagatt
     3661 aactatatgg aatgtaaaaa agaaaataag tttaaaaaaa ggtttcatta ttatatcgtt
     3721 ttagattaac tatatggaat gtaaaaccaa ttatgtacag tttagttact gtgttaagtc
     3781 ttgttttaga ttaactatat ggaatgtaaa atccttttat aaatatcttc tgacatgtgt
     3841 atatgcttgt tttagattaa ctatatggaa tgtaaatcgg ctacaacggt ataaattaaa
     3901 tttagcaggg tacattttag attaactata tggaatgtaa attgctgtga tttcaataat
     3961 aaaacaatta ataattgtgt tttagattaa ctatgtggat tcaaaattaa ataaacaaag
     4021 aaagcactta caaatatgta ggtgctttta ttctgctcaa aattggtcgg ttgggtaaaa
     4081 taattagaaa aagttagtaa aaacctattg actgtaactc gttacaatat tgttattaat
     4141 gtaacgagtt acagaaaaga ggtgaataaa atagcaacta aaagtagggc agagtatatg
     4201 aaaaatcgtc gaaaagataa aagaggtttt agtgtacttt tagacaaaga aaagttagat
     4261 aaatttgatg aagtgttaga ggaaaagaat ctaaccaaga aagaatggct agaagaaaaa
     4321 atcgacgagg aactggaaca aaaggaataa aaaataaggg tcactcccac cgaccaaagt
     4381 ttgagtaacc cctatgacgt atactatcgt atatcaatta tagtatatgt cattccttaa
     4441 aaaaatcaat tattaaggag tgtaatatta tgaaaaatga attaatgatg tttgaaggaa
     4501 aagagattga agtatttgaa tttgaaggaa gaattttatt caactcaaaa gatgtagtaa
     4561 actgtttaga cataaaaaat gttaatgaga atataagact tatgaatgaa aagcaagttg
     4621 taaagttaag aaattcggat atcagtaata ctgacatccg aaaacttaat aatgctggag
     4681 aaaattttct tactgaaagt ggagtgtata aattaatctt taaatcaaga aaagaagaag
     4741 ctgagagatt ccaaggctgg ataagtgatg aagttttacc aagcataaga caaactggtg
     4801 catacataaa taataatgca ataccgaaaa gttgagagaa aaagcaagtg agattgaaaa
     4861 attacagttg gtttataaca gtacatctat gttaaaagaa ttgctagatg atgcaggctt
     4921 tgacaataaa tcaaaactat taacagctaa aacattatat aagaaagcag gaattgattt
     4981 accaatagag ataaacgaag aagaacatta ttttgataca aaacaaatag catctaaact
     5041 gaaaatatat tctaagagta ataaaccagc tcagatggca gtttgtgaga ttattaaaaa
     5101 gattgattta gaagatggtg aagttaaagg tgtttgggaa attaatggtt cttggactgg
     5161 tactgtaaat aaatatacaa agagtgtaat agataaggtg agaacttgga tagaggaaaa
     5221 taatagacct accaagattg caggtgagaa gaagaattat tatgtatttt acaaaattga
     5281 gtaaatttat cagttgtatt aaataattta gtttagtttt ggggggatta atacaatgtg
     5341 tgagaattta cttgataata tgtatattga aaagagaaaa gaagaatata gaattaaact
     5401 tttgaaaata agagaaacag atatagatat atataataag ttagaaagta tagtatataa
     5461 actttctgag aaaaaattag agaaaaataa taaatagaca tataaagcac ttggatattc
     5521 tactgtttca agtgctttgt ttggtataaa atggtataat ggagatagag aattatatta
     5581 acccaaaaat ggagaaaata gagaagatga tttagttgac gctttatcag taggagtact
     5641 tgcaggtaag acagaatctc tagtactatt agttggaaat ggaataagtg atactcaaaa
     5701 aagtttaata aataaaagag aatttagaaa tataactcaa attggtggaa atggaaatga
     5761 gaaagcattt agtgaagtag aaaacttagt taaatagatt agaaggtagc aactagagtt
     5821 ggttgttacc ttcttttttt atactttctc tctctaagta ttttcttttt catatcttct
     5881 aatacatcta atgcagtagt ataaaattgt atattatttc tataaggtat agctattgga
     5941 agaatgccct tgttaactaa aaaaataaga agtacaataa ataaaagtgt cataatcatt
     6001 ctaataaaat aattagtgat atctgataaa tcaaatgtta agttaattgt tgtagaaaat
     6061 acaacagcca taatagatat atacaaagca aaaatggggt tactattttt ttgtaactgt
     6121 cctttaagcc tagattgctc tatttctata tcttgaatac tttttatgga atccatttca
     6181 ctataatact tataaacttt taaataatat tttttatact tgtttaaagc ttcttcatta
     6241 tctttaaggt ctttgtcact taactctgat ttaacttccc caaaatccat aaaatccctc
     6301 ctacaatgtt atataatata ctttaattta tagcatattt aagaacttat atcagcaatt
     6361 tgtcgaacga ttttagctaa aaatagagta taatgatagg cttattattt ataagatatt
     6421 attttttata tagttatata tttctttcta attagataat ataatgctgt tgttataatg
     6481 gctactaata ttattgctat tattgtaata tctaatcttc ttgtaaataa gcctattgct
     6541 atatactcaa gacctgttac atagcgataa agtacttcta gtttaacagt ttttttctta
     6601 tctatatttc catatattga ttctaattta ttaaaatatt ttttactaca aaaagcagtt
     6661 ttaggtttaa ttaaatatat tattgtagtt cctattataa ctattattcc aataagtata
     6721 agattgatat ttccaaaatc catagcagat acctcctatt aaatttgctt ttcttaatca
     6781 aagtatatca gtaacacaag tacaaagagc atctaaaata ttacaaatat taacaggata
     6841 gataaaacta tagtaaaaat tgtaataata aagcgaacga aaattatcat actaaaacat
     6901 aagacatgtt ataattgtat tagatagaag attaacatac accaaagcac tccttacaat
     6961 aaagagtgct tatttttttg aaattcatca atatataaac tatcaagaac cttattcaac
     7021 acaccttaaa atcgatttaa atagctcttt ttgataacat aatttatatg atataataaa
     7081 aaaatagaag tgtaccacca atacacttct atagctataa ctaagcattc tcttgctggg
     7141 ggagtgcttt tttcatttct ccaaacaaca ttatttactt cattagtaat tatcaaatca
     7201 atctcattat ctttcaaaat ctctgttgca gtacaaatta gattatctaa gtcaaaactt
     7261 cgagttatct ctagttgttt gttatttact tctcttttta gtatataagt tttaaaatac
     7321 atttatatca ccccttgtat ttactctttt ttctctaaaa atatattgat aagtaaatat
     7381 gcaacaaata aaaaaatctc aatgacacat agtgttatag ctataactag aagtgattga
     7441 tttttccatg cactataagc tatgatgaaa agaaatgtaa aagtaaataa tgtataattc
     7501 ataattttaa ctaagatatg acttgttttc attgttaagt acaagtctct ttcatctgca
     7561 ctatcgtgta attcttcaac aatgcctttt cttgaaaaag cattacaaaa attaaagatt
     7621 gctattacaa ttaaaagaaa tgatgaaatt aggaatcttt tatcttttaa aatgcaataa
     7681 ataactaaac tagcacaaca taacatacaa actataatac ccactataaa acttcgttta
     7741 ctctttactt tcatgttttt tatcctccat ttctttattt tcttttaaac aacatagttc
     7801 ttcaatcgtt gtaccaaaca cttgtgccat acgataggca agcataagag aagggctata
     7861 ttgctctttt tcaatagaaa taattgttct gtttgataca tggactaaat cagcgagttg
     7921 ttgctgtgtt agacccatat ttgttcgaaa ttctcgtact tttgttttca ttccataacc
     7981 tctttctata tgaagctaac ttcatgtgaa gttagcttca taatatccta aaatgagtaa
     8041 aatgtcaatc tcattagata agtattataa aacctaaaag aataatatgg taaaaattgg
     8101 atggaattat ttaccttaat tctcttgttt gtaaaacttt attgatatag aatttaacat
     8161 atacataaat cggcaaaggg gcgcgaatta tgaaaattag taaattacca gaagcggaat
     8221 taaaagttat gagatatatt tgtgaagcta acaaggtatt aacttctagg gaattagttg
     8281 ctgcaatgga agaaaaatat gagtggaatg aatcaactac atttacagtt ttgaaaagat
     8341 tagagcgaag ggagttttta agtacagaga aaataggcaa acttacacat tataatatac
     8401 tagtaaaaga gaaaaaatat ctaaggtttg aaacaaagga atttttgaag aacatacata
     8461 aaaattcaat ttcaagttta atgtcagcgt tacatggtga agacgacgaa gtggatgaag
     8521 ataaattaag tgaactcgaa gaatatttta agaatttgaa ataagaaaat gacaaattga
     8581 tacataataa ataaaatgaa ggtatgaaat tataactttt tctaccttca ttttgtttat
     8641 tattctgaac atgttaagaa aatgaaataa gtttgtattt tagttatata aattttaata
     8701 gaatatgtac agtatatagt agatagaatg tataaaaata gaatttaaat aagtagctat
     8761 tattgcagtg aaatgaatgt ttctaagtat atttgggtgg aagaatggtg gaagaaaatt
     8821 agtgaaatgt aattcttcca ccattcttcc acccaaaaaa taaaaactgt tcaataagaa
     8881 ttaatataca tcaattcagc tgaatgcaat caaaatactt aaatgaaagt aatctgaaaa
     8941 caacataatt caaatcaatt tattcaaata aacctcaatt tatcttaaat cttaagaaat
     9001 atttaagaat taggataata ttttcttaag atttatattt tattatatag ttataaggga
     9061 aagttacaaa aatgtaagcc aaagtaatat aacaatttag tcattgttga gaaagggagt
     9121 gaaagatatt gtataatata gataagtatt attatacaat agaggaagtg aaaatatgta
     9181 caatatatta gtagtagacg atgataaaga aattgttgat tcgatagaaa tatatcttaa
     9241 atcagaagga tttaaaatat ataaagcata tgatggaatg aaagctttgg aaataataac
     9301 agagaatgac attcatctaa tactaatgga tataatgatg cctaaattag atggaatcaa
     9361 agcaactgta aaaatacgag aagaaagaaa tattccaata attttaattt cagcaaaaag
     9421 tgaagataca gataaaataa taggattaaa tattggagct gatgattata taacaaagcc
     9481 atttaatcta ttagagctta tagctagggt taaatcaaac ttaagacgat atgtaacttt
     9541 aggtaattat gaagatacat gtaaagaaat tctaaaaagt ggagctttgg agttaaatac
     9601 actaacaaaa gaagttaaaa ttgatggtga aaacataaaa atgactccaa ttgagtataa
     9661 aataattgaa cttcttttag aaaataaagg acgagttttc tcaatagatg agatatacga
     9721 aaaagtatgg aatgaggaaa gttttaattc agaaaataca gtagcagtac atatcagacg
     9781 aataagagaa aagattgaaa taaatccaaa agaaccaaga tttttaaagg tggtgtgggg
     9841 agttggttat aagattgaaa aattttagtg gaaaaattat aaaagaaaag agtttaatat
     9901 ttaattgcat tttgatttta gtcattttta catttacaca aatgttagga attttaatat
     9961 gttttaattt tttctctgag agtttaattt actctatggg gattatgctt ttcttaaact
    10021 ttattttata taaaatactg acaatgtaga tgaggtgggc aacttggaga aatatagagt
    10081 attaaagtta gtttcattaa tcataatatc tatctcattt ccattaacta tattgttggt
    10141 gacaatagga cttgctgagc ctaaatacaa tatacaaaaa acttctacaa agagtgggat
    10201 ttttgatata tctgatgatt tttatgatag taatatattt gacgaaggaa caatggcaag
    10261 tctattaccc aagtttaagc tatctgtagg tggtaaatta actagtaaag aaaaaggtaa
    10321 attccaacaa gaaggtaaaa gtatagattt aagtagtagt gattctgagt atgataaaat
    10381 catgattaat gaacaagctt ctgaagaata tttaagttat ctaaaaaata taaaagtgta
    10441 tgttattaat acatacacag gagtatttta tacaaatacg tcatatgcta gtttatatga
    10501 ttttaaacaa aaaactaaga gctattgtaa tgtagagata ataaataaga taggaaagag
    10561 ctcatatatt aaaacaataa atggagaaaa atttgaactg aataatctga gtctggaact
    10621 aaatgaagta gatgaaggtt ttgaggcata tgtatctttt ccagaagagc caactataga
    10681 agatggaata gtatatacta attttcaaat atttaaacaa gctacagaaa aagtaagatt
    10741 attttttgta gctagtttaa gtttagcaat tatatgttta ttatctataa ttttatatat
    10801 caaaataaaa ggtgaagttt taaaaagaaa taatgtattt ttactaatat acagtaagat
    10861 acctatggaa tttaatgtaa tattagtatc tatttcaatt gcaattttaa ctatatatgc
    10921 tcttgctgta tcttatcata ttgatgtaat tgcattagtt tctttatctt tactgatagt
    10981 atcaaataat atatttttta taaatgtgca attgagctat ttagaaaata aaatgtgtat
    11041 atttaagaca agtattatag taagagtgat tttatggtta ataaaaactg taagggaggt
    11101 cagtaacgct ataaagaaag tatctttagc aaggaaagtt attgttatag tgataatatc
    11161 tttagcttta atcatggcaa ttagcactcg aagtataggt atgggattac tgttttctat
    11221 atgtattttt ttaggattta caatatatat aactaaaaga ctttcatatc ttagttacgt
    11281 aatagaaggt acagaaagaa taaaaaaggg agaattagat tataagataa aaattactgg
    11341 gaatgataat tttacgtctt tagctgaaaa tataaataat attggagaag gtcttgatag
    11401 agctatagaa gagcaagtaa aaagtgaaag aatgaagtct gaccttataa caaatgtatc
    11461 tcatgatttg aaaactccac ttacttctat aataaattat gtagatttaa ttaaaaaaga
    11521 agaatcaata caaccagagt atataaatga ttatataaat gtacttgagt caaagtcaaa
    11581 aagactaaaa ttattgatag aagatttatt tgaagcgagt agagtaagta gtggcaatat
    11641 agaattacaa attagcaaga ttgatttagt gcaactatta agacaaagta taggtgaact
    11701 tgaagaaaaa ttatctaaaa ataatttata tttaaaatta aatgttccaa atgataaggt
    11761 gtatatatgg gcagatggaa gaagaatgta tagagtattt gaaaatcttt tatctaatat
    11821 agcaaaatat tccttagaag aaactagagt ctatattgat gtatatgact atggtgaaaa
    11881 tgtaaaggta actatgaaaa atatatcttc ttatgaatta aattttgacc caagtgagat
    11941 tatggaaaga tttaaaagag ctgatgaatc aaggaatact gaaggaagtg gattagggct
    12001 tgctattgca agagatttag tagctttaca aggtggaaaa ttttatatag aaatagatgg
    12061 agatttattt aaagcaaatt tagagtttca aaaatatgaa gaagaaataa caaatatgga
    12121 atgagatagt atttaagtat atatattttt tttactgtcg aaagctttta ttataatata
    12181 ttagaatatt tacagtagag aatgaagagt aaaaacagag taactttaag tcactctgtt
    12241 ttttatttaa aatcaataat ataacaactt aaattaaaat tgtacacata gttttaaaat
    12301 aacttataca ataaaacatt atccaaatat cacaagataa ttccaaataa atatatagat
    12361 atcactcctg taataataga aataccaata gatttaaact tatatgcaac aagaagtaca
    12421 atcataaaag atataatttt agggttagct atagataaat atagactatc tccattaaaa
    12481 aatacatctt tagcagttaa ggaagtaagt attgctacag gtataaattt catgattttt
    12541 ttcaaaaaat caggcatctc tttttttgaa aaaattaaca taggaagcac tcttggtaga
    12601 tatgtaacaa tgtacattcc aattataata aaaaatatat aattattatt cataagattc
    12661 ctccttttta cagtaagatt cttctataaa gaaaccaatt aaagatgcta aaattgatgc
    12721 aactattatt actaaattgc ttttgaataa tgcatataaa ataacagaaa taatgattga
    12781 tgatataaat actacaatat aaatcttatt ttttatttga gaaactatta aagctataaa
    12841 catagaagtc aatatgaagc cagaaataga actgtctata gatatacttt ctcctaaaaa
    12901 tgctccaata aaattagcta aaactgatga aattaagcag aagaaattaa gataaatagc
    12961 atttctatca gaccaatttc cagcagtaaa tttttcataa ttaactgcaa aagtttcatc
    13021 tgttatagta tgacaaaatg gcaatataaa ttttggactt ttatttttta tgtacatgtt
    13081 caaattagaa ctcattagaa aatgtcttaa gttaactata aatgtagtca aaacaataga
    13141 tgttacactt gcaccagtag aaatcattga agctgcaatg aattgagaac ttcctccaaa
    13201 tactaaagtt accattagtg ctatttgaaa aggtgaaaga ccagattttt gtaatagtat
    13261 tcctcctact atacctatag gtatataacc taagaatata ggaattgaag caataaagct
    13321 ttcttttaat tgttgagttc tttccacaat aaatcactcc ttttgtatta aatgtattaa
    13381 agtattagta aaacagaaaa atagattaat tgtataattg caattataat taaaaataaa
    13441 tttggattaa tatataaaat actatacaac aaatagataa tcttaaacgg ttattaaatt
    13501 ttatctattt gttattatga tgtaataata catattaatc tctataagtt aattactatc
    13561 tggtgtaatt ttattttaac aacaaaaaaa aatacacaca attaaaatat tgtgtgcaat
    13621 acaattattt tttctcttct aatagtcttt ttaatacgct caaaccagct ttaagttctt
    13681 ctgtactttc tgtagcacat acagctattc ttattgcaga aattggtttt tcttttccaa
    13741 cagcaaaacg ttcagaagca tagacttgta caccttcttt aagtgcttgt atctcaaatt
    13801 tttctgccat tattccatca ggtagaatca accatctaaa tatacattct tcatttccca
    13861 aaagattata tccagataaa tattgattaa taatcttgtt tcttcttctt gcagatttac
    13921 ggcgtgcatt aattaattta tcggcttcac ctgatgtaat catgcgataa gcaatttctg
    13981 ttaaaaaata agattgagat agattaatgt tgtataatgc gcttgataat gcctctcgat
    14041 actgtttagg tgtagagata tatgctagcc gtaaactagg tgcaataatt tttgataaac
    14101 tcgtaatata aattgtttga tttggcagat aggaagccac tggatttaaa tgtgtttcat
    14161 taagtaaact atgaatagca tcctcaataa caattaaatt gtatttagat gcaatatttg
    14221 caatcatttt tcgtccattt tgtgacatta tatgtgtagt tggattttga taatctggta
    14281 ttatgtatag acctttaata ttttcatttt tacaagcata taatagtcct tcttcactaa
    14341 tctcattatc ttcttgttta attggaatta gttgaatccc aagcatttta gctgctgtct
    14401 ttattccagg ataagttaat gggtctacac ctattctatc tccatgttga aacaaaccag
    14461 ctaaaatagc aacaattgca ttttgtccac cacttgcagg aagtaagcta tctgatgtag
    14521 tttcaagccc agctttataa attaatttgg caatagcttc tttttgccat ttagcaccat
    14581 ctctttgtcc atattggaat atagttttaa aatctatttc cttaattatg tgtttaaaca
    14641 aagtattcat ttcttctagc gtgaaatctg gattcattgt gcccatttca ataaatgtaa
    14701 ttttattatt actggacatc aagaatagat ttgaacgtgt atcataagat acaaaagttc
    14761 cacttcctgt aactccactt attagacctt ttttttcaca aattttaaaa gctctagaga
    14821 tagtactcac attaacatct aagaaatctg ccaattcacg ttggggtggt aatttagtac
    14881 ctggtaataa aaatccattg ttgatatctt gttctagttg ttctacaagc gccttatata
    14941 atatttgccc ttttgaaggt ctctttggtt tccagttcat aggataattt tcaaaagaat
    15001 taattggcat aagttttcac ctcacattat taatttgatt ttttctttat ctaaaacaat
    15061 aaattctaaa gatacaaata aaaactgata ggaaataatt ctatcagttt ttgtctttta
    15121 attgatttca aatcttccta taatttttat accttcttta tcataaagag ggattgtttt
    15181 tttaaatata ttaaaatttc tttttcttgt atataaaaca gcttcttctg gtgtctttgg
    15241 ttggttatca caatcgtaca aatttctttt ttttaactaa aagcaataat acataaaatt
    15301 attattgcta atgatactct taatgtggta tatagtcata gtgtcacatg tctatcaata
    15361 gttttttcag taattattgt aacgaatagt aggactcata aaatacttag aaatatgttt
    15421 ttagataaat tttaaatata taatctaact tatatctata aattaacact attattctcc
    15481 aattccattt ctagtttttt tataaattta ttaaattctt ctggttgcct ttttccaaac
    15541 aatactctta caagagctga attgattttt ccaaatatat tcctcttgct ctcactttca
    15601 ataaaaataa gttttgtatt ttctaagtca atatgtacta attcatatct agtaatagta
    15661 ctttctcttt tattgcttgt ttttatctca tatactctat ttttttcgaa attagttatt
    15721 tcaaccttaa atcttttatt atttttttgc tgtacaaatg ttcctactgc ttttttctca
    15781 ttaaaattag gaaaatttag tcttgcagat tttgtaaata tattaaatat gtcttttgca
    15841 gaatatttta ataaaacttc agttttaaat gtattcatat cgttttccct tctgtatata
    15901 aatttaatat ttataagtta atttttaagt taaaatagta taatatgcca tattaaatca
    15961 tgttaaaact atttatatag ctaaaatctt acctatataa gtatgtatac tatcatataa
    16021 aaataaaatc ttaaaataga aagggttaaa aacacagttt attataattt ttattgttga
    16081 gtagatacgt actattatat atgattgagc aagtttttca atcataaatt tataaagtat
    16141 tttgcattaa aatttaaaca aagaattaaa ttatttaaga ttatgaataa ctaatacatt
    16201 tagtaaatta ctaaatatac aaaatgtaaa tacttatcaa aatgtataaa tacttatgac
    16261 atctagttac ttacagggtt taagtagaaa aaaatatcaa agtaagtatg gcatgatagt
    16321 tgcttatata tttatcaaga aaggagttat taagcatgga gttgagagat aaacttgtaa
    16381 acatcataaa tacagaaaat aaaaaagatc cattaacaga tgttcaaata gcaaagtttt
    16441 taagtacaac tagagagaac ataactaatt tgagaaaaga acttaatata agtaattcaa
    16501 ggcaaagaag atatccttat ttaaaatcag caatttcggc aatattacaa aagaataaga
    16561 atatttctgt atcagaaatt acaagggaat tgatgacaga aggctttaat atatctagaa
    16621 gagttgtgga ggaactttta cctaaagaaa gtttagaaat ggatgtagta gaggaatctg
    16681 aagaagaatc gaatgaccca tttgaaactc tgataggtaa taaagggagt cttagaaatg
    16741 ctgtagagca ggctaaatct gctatattgt atccaccaaa agggttacca acgttgatta
    16801 ttggggaaag tggagttggt aagagtttat tttctagaca tatgtatgag tttgcaaagc
    16861 aaaaaaagat agtaaaagaa agctccaatt ttgttgtatt taactgtgct gattatagtg
    16921 acaatccaca attattatta tctttgttat ttggatataa aaaaggtgca tttacaggtg
    16981 ctgaatatga tacaccaggt cttgtagaag aagctgatga aggagtttta tttttggatg
    17041 aaatacatag attgcctcca aaaggccaag aaatactatt ttctatttta gataggggaa
    17101 agtttaggag attaggtgaa actaatgctg aaagaaaagt aagtattatg ttgattgggg
    17161 caactactga aaatgtagag actaatttat tattaacatt cagaagaagg ataccaatgc
    17221 taataacttt gccaccatta catgatagat tactaaaaga gaaaatagac cttatataca
    17281 atatctttca acaagagtgt aatagaataa atgcaaaaat atttgtagat aaaaatgtga
    17341 ttgaaatact tgcactaaaa aagtttagtg gaaatatagg acaattacaa aatatgattc
    17401 aggttctttg tgcaagagct tttatgaagt ttataaattc caaagatgaa gatagtgaat
    17461 cgatagtagt agtagatata aatgaagttt taaagcttaa agattctttt aaagatgctg
    17521 cctttcaaga gattgaatat acagagataa gaaaatattt gaaaaatgct atatttatac
    17581 catttaattt agaagaatca tcagtaaaag gaagtctttt aagtgatgaa tatatattac
    17641 cagaggacat atacaaaaca attgaaaaga aatattatga cctaaaatcc ctagacattg
    17701 aagatataga tatagagaat attttatgga cttttatttt aaatagattt gctgacttaa
    17761 agtttaatct cgatagtaat gaagaaattt tttcaatgag tgatttaaat agttttgtaa
    17821 gtgatagttt agttaaactg gtaaaagaac ttatgaatga tatagttgaa aaaaatatga
    17881 atttagaagt aaataaaaat atgttcaaat atctagcaat acacttagaa gaagctataa
    17941 aaagaattaa gctaaatcaa aagataataa atgtaaattt agaaaaaata aagattgact
    18001 tttcaaaaga atatgaaatt agtaaaagtt ttgcaataaa aattgaggaa tcaattggta
    18061 ttaagattcc agatgatgaa attggattta taacactcta cataaagtct gctctaaaaa
    18121 atgaagtgaa aaaagataaa gtagggttaa taataatttc tcatggacgt atagctacag
    18181 agactgtaaa tgtagttaaa gaacttttag gtgtaaagtt tcctgtggct atagatatgc
    18241 ctttagatga aaaaccaata aatatataca ataaggcagt agaactgtca aaaataatag
    18301 accaagggaa aggcatattg tttttggtgg atattggttc acttacaaac ataggtcaaa
    18361 tagtaaataa gagaaccaat ataagcacta aaactattga tagagtagac ctcctaatgg
    18421 cactagaagc aactagaaaa gtttctatgg gagaggaaga acttgatgaa atattttttt
    18481 ctttgctaaa agataggatg ggatataact atgaattaga aaaacacata gataaatcaa
    18541 atgcaattgt aacattatgt cttactggag aaggaactgc taagtatata agtaaaacgt
    18601 tggaagaaaa gtatgataac actaaatgtt atcaaatgag tgcattggat gaaaatttat
    18661 tttgtaaaat tgaaaatcta aaagagacaa acaatatatt agctattgtt gggacaataa
    18721 accctaagat accaggtata aattttatac cctataataa agaacttttc aaaaatttgg
    18781 atatctatct atcaaaatca aaacaacaag atgacagacc agttaaatcg tatgaaagaa
    18841 tgcttgatga ggatttagtg atttttgaac cagatatata ttttaaaaag gatttattag
    18901 aatatgtatg ttcaattctt ataaataaag aatatgtaga aaaagattac ttagattctg
    18961 ttttacatag agaagaaatg cttccaacat attctaaagg agctataggt gtgcctcatg
    19021 gagattcttc tactgtaaat agtacaaggt ttgtttttgt taaactaaag aatcctattg
    19081 attggggtgt aggtaatgta aatttcatat ttatgccagt ttttcaggct aatgataaag
    19141 agattgttaa aaatgttctt gagatactaa aagatgcaga atttatgaac aatgccaata
    19201 agtgttttga taaagatgct tttagaagga ttatatttga taaatttaaa catctgtagc
    19261 catagcaaaa aaattgagag gagttagagt aatgaatgta gatttaatat cattgggttt
    19321 agaatctaat gattatgaaa gtgttattga agaattagga agtataatgt gtaaaaaaga
    19381 atatgtaaaa gaaacataca taaatgctgt cttggaaaga gaaaggacat taccaacagg
    19441 tctagatatt ggagaaatgt gtgttgctat tccacataca gattcaaagc atgtaaatga
    19501 gtctaatgta gctgtaggga tattgaaaaa tcctgtaaag tttaattcaa tgatagaccc
    19561 aaaagataga cttgatgtag aacttgtatt tttattagca gtaaagaacc cagattcaca
    19621 agtaaaactg ttaaaggatt taatgtcagt ttttcaaaat ataaagttat taaaaaatat
    19681 aaaaaacgca tcaacaaaag aagaagtagc taaattattg gattttatag aaatttaatg
    19741 taggagaatt ttataaataa aaaattaaaa tttaaacaat caaagtaagt attatttttt
    19801 taataggttt aatcttatat aaaataaaaa tttagagtga aaaattagga ggaaattatt
    19861 atgaaacaat ttaatgtatt atcagtttgt ggttcaggga cagttacatc atctatggta
    19921 gcaggtaaat taaaagaagt actaggtaat aaggggattt ctattactac aacagaagct
    19981 agaccaacag aagcactaaa tttagcacaa tcaggaagat ttgactttat aacatttacg
    20041 agtccacttc aaacaggcga ttatggaata ccaactataa atgcatttgc ttgcctaact
    20101 ggtatagggg aagaggcatt ctttgaagaa gttttgactg ttattgaagg gatagataag
    20161 taagttttgg agacgtgaat ttatagtatt taaatcaatg atttagatac tataataagc
    20221 taatattaac taaatatttg tggaaaaaac attttatatg ttgtcataaa ttaaaaaatt
    20281 ataaaaatag tcacttaagt attaaattaa gcttataaat atttataagt aggagggaaa
    20341 aagatgttag aatttatgaa atctataatc gatacatttg gttctgcaat tatagttcca
    20401 attataatat tcataatagc aaagatattt aaagtaacta ctaagaaatc gtttctttct
    20461 gctgtgtatg caggagttgc tttacaaggt tttacactga ttttaaattc atttacacca
    20521 ataataacac cagttatcaa taggatggtt gagagtacag gagtaaacct accagttttt
    20581 gatgttggtt ggcaagcaac atcattggtt gcattttcaa caagtgcagg tatgatatat
    20641 ttaggtcttg gtatattatt acaaacaatt ttatttttga taaaatggac agacgtgttt
    20701 caaccatcag atttatggaa taactattct tatatggtat ggggagcaat ggttatagga
    20761 gtaacaggaa attttccttt aggtatagca tgtatggttt tattaaacct atatagttta
    20821 ttaatatcag agttagttgc aaagagatgg tctagttact atagatatcc taattgtaca
    20881 ataatagcta tgcataatgt tgaagcttct gtgtttgcag tatttgcaga ccctatatac
    20941 aataaattgg gattaaataa aataaaatta aatccaaaag aattagagaa gaaattaggg
    21001 tttttaggtg aaccaattac tttaggtctt tttttaggta tgtttatagg tatattaggt
    21061 aatatgacga gaataaatac tatggaagct tggggagaaa taatgaaagt aggtatatct
    21121 acaagtgcag tgatggcaat tttccctaaa gtagcaagta tgtttgctca agcatttgca
    21181 cctataacag aagcagctag aaagattatg caaaaagctg gaaaccgtga gtggtacata
    21241 gcagtaaatg atgctgttgg gtatggtgaa cctgcaactt taatatcagg tcttatatta
    21301 attccaataa tgcttgtaat agctatggta cttcctggta ataaggtatt accagttgtg
    21361 gatttattgg caattccata tatggttcaa ggtttagttg ccatacataa tggtaatata
    21421 ccaaaagtat tggtttcagg tataatttgg tttggattag gattatatgt ttgtacatct
    21481 acagcaccat tatttacaga tatggctaca aatataggtg tagctatacc tgcaggagca
    21541 atgttaataa caagctttaa tatactagga aaacctttga tgggattagt tttctttgca
    21601 tttctaagtg caaatccaat ttatataggt ctttcagttg taatttactt tgttctatgg
    21661 gcattgttta gaaagaacaa aacaagtatt cttgattatt tagaaaaaca agcacttaaa
    21721 aatgtagaag aagagcctgt agcagtttaa gtttataaaa atgaaataag aataattaaa
    21781 ggagtttata attgatttta tataagtatc agataatacc tgagaaatat ttaaatgaaa
    21841 tatagacttc tttttatttg ataatatttg taattataat gcattgtatc tttatagttg
    21901 agttttctgt ataacctgct atttaaaaca tactctttta tatttataca gtaatgtggt
    21961 ttaattttat aaaatgtaat ctctactggt aaaagacttt gtatataagg catttgcctg
    22021 aataaaccat ttatcttttg tatggcatag atattgctat atagaagtat atagcatgaa
    22081 aggagatatt tatatgggag ataaaatgag agcatcagta ttgtatagtg taggtgatgt
    22141 cagatatgaa atggtagata taccagaaat aacagatacg caagtgcttg taaatgtaaa
    22201 atatgttggg atatgtggtt cagatttacc taggtctatg gtaagtggtt tgagtggaaa
    22261 tacaaaatat ccattgattt taggacatga attttctggc gaagttgtta aaattggaga
    22321 aaaagtaaag catattaatg taggagatag agtagctgtt gcaccattag taccttgtgg
    22381 aaaatgtgat tattgtaatg aaggtaattt tggcttgtgt gatgattaca atataattgg
    22441 aacaagagta aatggagcgt ttgcagaata tgtaagagtt ccagaagaac atattttaaa
    22501 actaccagat acattggatt atgaaactgc tgccggaatt gaaccagcaa ctatagctta
    22561 tcatgggatt tcaaagtcta atattagagt aggagatagt gtagttgtgt taggatgtgg
    22621 tccaataggt cagtttgtta tacaatgggc aaaggtgttt ggtgcatcta agattatagc
    22681 agttgatatt tttgatgaaa aattagaact ttctaaactt ctaggagcaa actatatact
    22741 taactcaaaa gaagtaaatg taatcaaaga aataaaaaaa ataacaaatg gtggtgctga
    22801 tgttgtaata gagactgctg gtagccgatt tacacaagaa caatctctat ttgtagcaaa
    22861 aaagagggga aatatagtgt ttgtaggtat atcacacaca gaattaccac ttagtgcaga
    22921 cgctactgag tgtatattac gtggagaact taccttgaaa ggttcttgga attcatatac
    22981 aagcccttat cctggaagag catggactgc aacattggat tttatgggaa aaggtgacat
    23041 tatttttaaa cccatgattt cacataaaat aggtcttaat gaagtgggtg actttttaag
    23101 taaaatgagc aagagagaaa ttaatttcaa taaaatccta gtagaaatat aaaaatttaa
    23161 taaaaaattc taaaagcaat taaattttat aactatgaca ttaaataaaa taaaaataat
    23221 tttcttaaat aaaatatagg aggaagaaaa gtgttagaaa atttaaaaga agaagtatta
    23281 aaagcaaact tagaattacc taagaaaaag ctaattactt atacttgggg aaatgtaagt
    23341 ggaatagata gagaaaaagg attagtggtg ataaagccaa gtggtgtaga gtatagtaat
    23401 atgactgtag aagatatggt tgtagttgat ttagagggaa atatagttga tggcaaatta
    23461 agaccttctt cagatacccc gactcatcta gttttatata aagagtttga agaattgggg
    23521 ggaatagtac atacacattc aagttgggca acaatttggg ctcaaatagg aaaatctata
    23581 ccatcttgtg gtactactca tgcagattat ttttatggaa gtataccatg taccagaaaa
    23641 atgactagag aagaaatttg caatgaatac gaaaaggaaa ctggaaatgt aatcattgaa
    23701 gaatttatgg gaagaaatcc aattcattgt ccaggaataa ttgtaaatga ccacggacct
    23761 tttacatggg gaaaagatgc aaatgaggct gtacacaatg cagtagtttt agaagaagtc
    23821 gcaaagatgg cttattatac agagttaatg agtcctgata atattatgga taaggttctt
    23881 atgaataagc atttttcaag aaaacatgga aaaaatgcat actatggtca aaaatgatta
    23941 tctggaggtg aattatgcta gactctttaa aaggtaaaat tgtggaaata gcaattaaag
    24001 cagagcatta tggtttatgt aaagaaaaga ctggtaattt tagtattaga gataaggata
    24061 ctggatatat acttataacc ccatcaggtg taggaagaga agaattaaaa gttgaacata
    24121 tttgtgtatt ggatttggat ggaaatgtaa tagaagttga gaaaggtatt aagccaagta
    24181 gtgaggtatt gatgcatatt gaaatataca aaacaagaga aaatataaat gccatatcac
    24241 atactcattc tttatatgca acagcatttt cagtagcaaa gaaaccaatt ataccaattg
    24301 tatatgaatc agttaattat ggcggttatg tagatattgc tccatatgaa agaccaggaa
    24361 ctttaaaatt agctaagagt gtttcaaaat tactgataga tacagatgca tgcttattag
    24421 aaagacatgg gttgacaact gtaggtaatg atttagacga agcattatta aaatcaagat
    24481 atgtggaaga ggtagcagaa atatactata ggtctttagt attaaatcaa tttaaagaac
    24541 ttgaagtagt agatgttgat gagtttaaat cttggaaata cccagaagaa ataaagtttt
    24601 aatctaaata attttcattg aagtggttaa ttaaaatata tgtattattt atatcaatta
    24661 ctatgtttta ttatgttaat taccgtattt tgcaaatata tttattacat tttttcaaaa
    24721 aaatatagaa tccgaaagcc atataaaatt gacttgggat tctatacttt ttatttatgc
    24781 atttttcttt atctgatttt ctggagaatt tatatgttca aaatattttt tagtttcaga
    24841 tactattata ggtgataatg caagtaaagc tataaggtta ggtattgcca taagcccatt
    24901 tacaatatca gcaattatcc aaacaacttc taactttaag aaagcaccta ataacaccat
    24961 agccacaaat acagttctat acccattcat gccttttaca ccaaacagaa attcaaaaca
    25021 tctttctcca taataactcc atcccaaaat tgtagtaaat gcaaataatg ttaaactaac
    25081 tgtaagtaat attggtccaa atgtaggaat tgctccgtta aaagctgctt gtgtcataac
    25141 agctccattt aggtctgaac accacactcc tgaaataaca agagagaatc cagtaagagt
    25201 acagattata atagtatcta taaaagtacc tgtcatagat ataagacctt gttccgctgg
    25261 ccattcagtt ttagcagcag cagcagctat tggagcacta cctagaccag actcatttga
    25321 aaatacacca cgagcgatac catttctaat tgccagtgac atagttgctc ctaaaaaacc
    25381 accagtagca gcagtaggtg taaatgcact tttaattatt aacataaaag tttcagggat
    25441 tttattccaa aaagatatta aaatgattcc acagattaca acatatataa ctgccataaa
    25501 aggtactatt ttagtagcta cattagatat acttttaagt cctccaaata caaccaaagc
    25561 tacaaatact gctaatacaa cccctgtaat tctagggtct atcccgaaac tattatttat
    25621 tgcatctgtt attgaattta cttgagtaaa agtaccgatt cctaaaagtg caactagtat
    25681 accacttata gcgaaaaata ttgcaagagg tctccatttt tctcccatac catttactat
    25741 gtaatacatt ggcccaccag acacttggcc atttttatct ttagttctgt attttattgc
    25801 aagaacacct tctgaatact tagtagccat tccaaagaat gctgctatcc acatccaaaa
    25861 aagtgcacca ggtccaccag ctttgatagc tgtagcaaca ccaacaatat taccagtacc
    25921 aactgtagca gctagagcag tacataaggc tgcaaaactt gaaacatcac cactaccttt
    25981 gttttcagca gagaatatta acttaagagc tcttggcagt ttagttattt gaacaactcc
    26041 aagcttaaca gtaagtagta taccagtacc aactaatagt accagtaggg gtggtcccca
    26101 aataaaagca tcaactttgt ttaaaatatc aattagattc ataaaaacac tccctcattt
    26161 tttttgcttt acataatcaa tagaattttt aatgaagtaa ttgacaggaa ataagtataa
    26221 tttattaata atttctagtg taataaaaaa agctaaatct aaaaaagatt cagctctgga
    26281 tgtacactaa ttagcaacca aaataaaagt attagtaaat actgtttttg gtagataatc
    26341 cctgtccttt tgcctgagag attgagcata aaagccttgc cccttcggcg cccataatgg
    26401 gtctctccag agttccgtaa attttacagt cctactttta aaagtacctg agagtgttac
    26461 tccttcggtg tgctatatag caactcttct ataaaatctt aaccggatat tgatttgtca
    26521 tagcttattg tattaataat attaatataa gtttttatgt tttgtcaact aaaaaattaa
    26581 aaaacaaaga aaaaatataa aaatgtaagc ctatttacga attataaact tagaaagatg
    26641 agtaatatac gtgaaaatga attttgtaat acacatacaa atgaagtgta aaataagttc
    26701 taaataaaaa aataataaaa tcttaaataa ttaattagat atgattaaat tatggtaata
    26761 ttatagttac gatattaaga aaagttctat ttagttagat tttaagcgta atatgagatt
    26821 atgtatagag aaaggatgta tttttagatg ggaaaaatat tagtgctagc agaaaaacct
    26881 tctgttggaa gagatatagc aaaagtttta gggtcaaaaa atgaaaaaaa tggttatata
    26941 gaggggccaa agtatgtgat aacttgggca ctaggtcatt tggttacact tgctgacccc
    27001 gaaagttatg gagaaagata taaatcatgg agcttagaag acctgccaat acttcccaag
    27061 caccttaaga ctgttgtaat aaaaaagagt ggaaagcagt ttaatacagt taaatcgcaa
    27121 atgaatagaa atgatataga tgaaatagtt atagcaacag atgcaggtcg tgaaggagaa
    27181 cttgtagcaa ggtggataat agaaaaatca caggtaaaaa aaactataaa acgtctttgg
    27241 atttcttcaa gtacagacaa agcaataaaa gacggatttg caaaattaag aagtggaaaa
    27301 gaatatgaaa atttgtatta ctcagcaata gcacgagcag aagctgattg gattattgga
    27361 ataaatgcaa ccagagcttt gacaacaaag tacaatgcac agctttcttg tggaagagta
    27421 caaactccta ctcttgcaat gttgcttaaa agagaagaag aaataagaaa tttcaaacct
    27481 aaagagtatt atggattaga attaattgct acaaagggaa attcagatat aaaatttatt
    27541 tggaatgata aaaacaataa ttcttcaact tttagtaaag aaaagataga atccacatta
    27601 aaaaaagtaa aaggtgtaga cataaaaata tctgaagtaa acaagtcata taagaaaaaa
    27661 tattcacctg cactctatga tttaacagaa ttacaaagag atgcaaataa aatgtttgga
    27721 ttttcagcaa aagaaacttt atctataatg caaaaactgt atgaacatca taaagtactt
    27781 acttatccta gaactgattc tagatattta acatcagata ttgttggaac attaaaagat
    27841 agaataaaag ctgtaaatat tagtgattat tcaaagactt gtaataaatt attaaaaagc
    27901 aatattatag ctaataaatc gtttgttgac aattcaaagg taacagacca tcatgcaata
    27961 ataccaacag aacagaaggt attcatgagt gattttagtg acaaagagcg aaaaatatat
    28021 aatcttgtag taaaaagatt tttagcagta ctatctcctc cttttgaata tgagcagaca
    28081 actataagtg caactgtaga aggagaaata tttaaggcta agggaaacaa agtaataaat
    28141 ttaggttgga aagaaagtta taaagatatt gaagatgaag atgagtatga aagtatacca
    28201 aatattttag aaaaagatgt acttaaggtc aagggattaa agataacttc aggaaaaaca
    28261 aatccaccac catacttaaa tgagggaaca ttattgactg ctatggaaaa gaatggtcta
    28321 gggacagttg ctacaagagc agatataata gaaaaattat tcaattcatt tttaatggaa
    28381 aagaaaaata aagatatata tataacatca aaaggaagac aattattaga tttagttcca
    28441 gtagacttaa agacaccaga acttacagct tcatgggaaa agaaattatt ggatatttca
    28501 aagggaaaac ttagtaaaaa tacattcata aatgacatga aagattattc aaaagatatt
    28561 gtactagaag taaaaaatag tgataataaa tttaaacatg ataacttaac taaaaataga
    28621 tgtccagaat gtggtaaatt tatgttagag gtaaatggta aaaaaggtaa gatgttgatt
    28681 tgtgaagata gagagtgtaa tactagaaaa actatatcac aagtaaccaa ttcaagatgt
    28741 cctaactgcc ataaaaaatt agaacttcgt ggagaaggtg aggggagaat cttcacttgt
    28801 agttgcggat acagagaaaa gttgtcgtct ttcaacaaga gaaagcaaga agaaaaacag
    28861 aaggcatcaa aaaaagatgt taatcaatat ttaaaaaatc aaaataaaaa tcaagagaca
    28921 tttaataatc catttgcaga agcattagct aagctaaaaa acaaataatt ttaaagttat
    28981 tattatacac aattataact ttaactgtat attagttata tgtactgata taatatagtg
    29041 tatagtttag gcatgaaagt agggctgtta aatggattta agtggcatat ttaaatacta
    29101 ttgtaaagag tgtgaaaata catggaataa ttcgagtgtt gaattatttg agaatataga
    29161 aacgtatagt aaagattcac aaaaaaagag ggaaaaagaa ttagataaat tgctaaatac
    29221 aatatcagtt catttagaga ggtatccaag tgatgctgta ttgagaaaaa tgtgggtaaa
    29281 aaagggcgag gttttcttac aaaagacatt ggaaaaagaa aatattttta agttagaaaa
    29341 aatggatgta gaggatagaa aaaaattttt agatataaca aaacagttta ttagagatgc
    29401 tagaaaattt gatgatgatt tacctatagg tgatattatg caagctatga ggaatgtatg
    29461 gatttcaaat gcattacaat tattatttgg taaagaagta tattattcaa aagctaactt
    29521 tgcatatagt atgttatatc catatactga taattattta gacaatacaa atatagataa
    29581 aaatgataag attttattta ataactggtt agaaaaaagg ctcctgggag aacacattaa
    29641 atctaaggat tatcatgaaa gtaaagtatc taaaatgata gattatattg aaagtgtata
    29701 ccctagagaa aagtttacag aagtttatga atcgttatta ttaatattta aaagtcaagt
    29761 aaatagttta aaacaacatg gtaaggaaaa tcatttgtgt aaagaagatt tattatccat
    29821 ttctattgaa aaaggaggtt catccgtttt agtagatgga tatttaataa gtggattgat
    29881 gacaaaggaa gaaatagagt tttgtatagg atatggattt ttattacaaa tatctgatga
    29941 tttacaagat ataaaagaag atttaaaata caaccataaa actattatta cagagatgtc
    30001 aaaagagggt actttagata aagttgtaaa taaactaata aattttacta ttgagttaat
    30061 agatagtttt aaaattaata ataaaaataa atctgtaata actatgataa agaatgattg
    30121 cttaatgtta attttatttt ctgtagttta taatgctgaa tttttttctg taggatatat
    30181 aaaagaagta gagaaattta ttccatatac aatagattat tcattagaga ttgaagaaaa
    30241 aataaaagaa aaatttaaaa atatagatgt tttaaataat gaaaatgaat ataaagaaat
    30301 gattgatatt atttgtgcag agtagaaaaa aagggagtgt cttagaatag actttttagt
    30361 ttatgaggca ctctttttgt ataaaatcaa atgtattttc atcattttaa taattaaaaa
    30421 gagcttatcc ctatttgaga aagccctttt taatttaata aaaatgtcaa ggttaaacta
    30481 tttaaatgca gtaataatcc cattatcatg taagtttttt atgattgtag ctgcgatagg
    30541 gaatccaaat aatccagcta ttccaaatag ttgagctcca acaaacatac acattaatgt
    30601 tattaaagga tataggccaa tttggtgtcc tactaccttt ggttctaata tttgtctaac
    30661 aacggttata attatgtata gtattaaaag tcctattgct aaattaatat ttccattaat
    30721 gagtgagata atcatccaag gtgtcaaaat acttcctgta cccaagactg gcaaaatatc
    30781 aacaagtgct atgatgacag atatagtaaa tgcattttca acctttagga tagtaagtcc
    30841 aataagtagt tctatgaatg tcactgatag tagtattgca taggctttta gtagttttaa
    30901 aagagcatta atactgctat tttttatatt tattatcata aattgacttt tttctggaaa
    30961 ttgttttaag ataaagttta ctattttttg atagtcaaaa gtaaagaaaa aagaagcaat
    31021 taaagtaata ataagtttta acataaatgc aggtgtttta cttgcaatac tagcaatagc
    31081 attgagtgca tttgttgaaa tagaagctac taaagacatc atagattgac ttatattatc
    31141 ccaaccaagt attaatgcta ggttaacatt aggtgtacta gcatctatct ttgaaaataa
    31201 agtatttagc gttggttgta tacttgattt atatatttca ggaagtctaa aaaatacatc
    31261 acttacactt gcaaatatct tcacaccaaa gctaactaat aaaaatatac acaacccata
    31321 aaaaattaaa agtattacta tagaaataag tgtacgattt aaatttgtat tatttgtaat
    31381 aagtcttata ataggtctta agatggatgc aatgataaaa ctgattacaa aaggcattaa
    31441 taatggcaat aagaatttta atacaatata tacaatactt gttattaaaa ctacatatgt
    31501 aaatttaatt ataaaattct tttgcttttg aatatccata atataaaacc tccaatgtat
    31561 ttgatattat tctaaatatg gtctttttta cagaaaatat actactgtat attgattata
    31621 aactataaag cttatgtaaa gtaaataaat tatgtaaaat ttaggttaat tttgtataaa
    31681 gagatacttt tatattttac aattaataac caaatactaa aaaatatttt aaataaagaa
    31741 cttaaaattg tgataaaatt aatttataga tattaaaaaa agtataatat atggttttta
    31801 agaggtgcta agatgaaaaa ttttagagat ttaggtggga acaaaactga ggatggcaga
    31861 actgtaaaga agggattatt ttataggtct gcaaaattat caaatctaag tgaaaatgat
    31921 ataaagattt taaaagagtt aaatattaaa tatatatttg attatagaag tgatgaagaa
    31981 gctcgtaaac atccatcaac aataatatca aatataaaaa atataagaat accagcaatg
    32041 agagagctag aagaatcagg aggaagtttt ggttctattg aagatatgat tgatggattg
    32101 tttgaaaaag atggtgcatt taatatgtta aataatagtt actataattt gcctataaat
    32161 aatccgtcat ataaaaaatt agtagaactt ataagagact attctaattt accaatatta
    32221 aatcattgta cagctggtaa ggatagaact ggtgtgggga gtgctataat tcttatgatt
    32281 ttaggggtat cgagagagaa tataatgaaa gattatctaa aaagtaatga ttttgcagat
    32341 aaagagatag aaagatttat agaatataag cctaaattta aggacattcc aaaagaaaat
    32401 ttgaaatata tatttggtgt aaatgaagag tatatgaaaa ctgcatttag aagaattgat
    32461 gaagaataca ttagtgtaga agcatattta tatggagaat ttaatttaaa taaagaagaa
    32521 ataagaaaat taagaaatca atatctagag taaaaggtga gtttatgagt ttaaatataa
    32581 cagcaagaca gataaatata ataaaaatat tgttaaattc aaaagataca attagtggta
    32641 ttgccttaag tcaagagata ggatgttctt caaaaactat acaaaatgaa ataaaggata
    32701 ttaataaaca acttagaaat ggtaaaattt tgtcaataag aggagttggt tataaactag
    32761 aaggaagttt tgatgaggta aatctaaata gcaatctcta tgatgatgta gatagagtag
    32821 aatatataat cagaaaaata ttaaccttaa gtaatgaaga aaaaaatact ataaaacttg
    32881 aagatttagc cgattctatg tatgtaagtg tttcaacagt aaaaaatgat ttaaaagaag
    32941 taaaaagaat gttggaaaag tattcaatat cagttaaatc taagcataaa caaggtatat
    33001 gtgttttaga aagtgaagat aagatattaa attgtatagt tgacttaagc aataagagag
    33061 ataatcaatt gagtttaaat gattttttaa atgatgatat aaaggaaaga aaactattaa
    33121 taaaaaaatt attattagac atattaaatg aaaatggatt agtattaact gatatagaat
    33181 ttaagagtgt gttaaataat atattagtta tgttaagcag acataagtat aatgaaaaag
    33241 aatttataga gaattatata gaaaattata ggagcaaaag aagctcaatt ttagaaaatg
    33301 aacaaaacaa agaaattata ataaattcaa taaaaacatt ttgtaaaaat ttaaaattag
    33361 ctacatcaat agatataagt aatgataagg tatttgaaga gtttttatat aagcatatta
    33421 gtagtttatg taaaaaaata aagttaggta taaatcaatc acctataatg gctcaagata
    33481 taaaaataaa atatccattt gcttttgaac tagctaatat agctaaaaag acaatagaaa
    33541 aagatttgaa gatagaaatt aatgaagatg aagttgcaaa catagctcta catataggag
    33601 gagcagttga aagagcatct tataataata aagataaggt tttcaaaact ataatagttt
    33661 gtacttcagg cataggcaca tcaatgttaa taaaagctaa attagagaat atatttaagg
    33721 aaaaattaga aatactaaag attattcctt catacttagt tgaatatata aaagttatag
    33781 atgttgattt tgtaatatct actgttccta ttgaagtagg agatgtgcca gtaataaata
    33841 tatctccaat tttaagtgag aaagaaatta gattaattga aaaatatata gaaacaggaa
    33901 atatatacct agatttagat ataaaaagcc tgtttgatag tgaattgttt tttactgatt
    33961 tggtgtttaa aaggaaagaa gaagttatag attttatggc aagtaaactt gtacaaaagg
    34021 gctatataga tgaggatatg aaaaaatctt actttgagag agaaaaaata gcaacaacag
    34081 aaatagggaa tatggtaact ataccacatg gagctattgg aaaaattttt gataataaaa
    34141 tagcaattgg tatattaaag aagcctattt cttgggaaat atcagatgtt cgtttaataa
    34201 taatgttagc ccttgataaa gataaaatat tagattatga agtaatattt tcaaaaatat
    34261 ataaaagagt agattctata gcaaaagtta taagtatatg tgaaaatagt agttttgaaa
    34321 aatttgtaaa tttatttaat tagatgggta gtattgtata tacacctgta aatttggata
    34381 atataaaact aaataagata aaaacgataa atttctcttt taattaagag aaattttttg
    34441 actttcaagg atattaatat aaagttaaga tataaacata aagcaaataa ataaaaaaga
    34501 ggtggcaata tgatttatac agtgacatta aatccatcaa tagattatat agttaagcta
    34561 gatgagctaa agacaggaag tacaaatcgt gtaaaagaag aatacgttta tccaggagga
    34621 aaaggtataa atgtatctcg catacttaag gaacttggaa atgataatac atctttaggt
    34681 tttataagtg gttttactgg agaatatata ataagaacat tggaagaaaa agaattgaag
    34741 acagatttta taaagataaa aagtggtttt tcaagaataa atgtaaaaat aaaggcgtta
    34801 aaggaaactg agattaatgg acaaggtcca aatatagatg atgaagacat tgatacttta
    34861 tataaaaaac ttgataagct aaatcaagat gatattttaa tattggcagg aagtatacca
    34921 tctactttag atgaaaaact atacgaaaat ataatggcta gattagaaaa aaagaatata
    34981 aaagttgtag tagatgcaac taaaaatctt cttcttaatg ttttaaaata caaacctttt
    35041 ctaattaagc ctaataacga cgaattagaa gaactatttg gtgttaaatt aaattcaatt
    35101 gaagacatgg taaaatatgc tagaaggtta aaggaaatgg gagctataaa tgtcttggta
    35161 tcaatgggaa aagatggagc attgttgatt acagaggaag atgaagtgct tataagtgat
    35221 gttccaaaag gaaaagtaaa aaattctgta ggagcaggtg attcaatggt agcaggattt
    35281 atatctgggt atttgaatac tgggaaatat gattatgctc ttaaattggg agcagcttca
    35341 ggtagtgcaa ctgctttttc atatgactta gccaaaagag aatacattga taaattggta
    35401 aatgaaatat ctgtaaagaa gttttaaaat attaaaaaat atatagaaag gtaattaata
    35461 tttatgaaaa ttatagattt attagatgaa aagtcaatta agttaaattt aaattcaaaa
    35521 accaaatcag aagcaataga agaattagta gatttggtcg caaatagtgg aaatttaaat
    35581 gataaagaaa actacaaaaa agcaattttg gctcgtgaag aaatgagtac aacaggaatt
    35641 ggagaaggtg tagctatacc tcatgctaaa aattcttcag ttactaaagc ttgtatagca
    35701 gcagcagtgt caaaagaagg tatagattat gaatcgtttg acggaagttt atcaaatcta
    35761 ttttttatga tagctgcacc agacggagct aacaatactc atcttgaagt attatcaaga
    35821 ttatctacaa tacttatgga tgaagacttt agaaataaac ttataaattc aagctctgaa
    35881 aaagaattta aggaaataat agataaaaag gaaagagaaa aattcagtga agaatatcaa
    35941 gatgagaaag tggctgaaaa aaatataata gatactaaag agaatgatgc taataagtat
    36001 cctaaggttt tagcagtaac agcatgtcca actggaatag cacatacatt tatggcagct
    36061 gaaagtttaa ataaaatggc agaaaacaaa ggtgtatcaa taaaagtaga aactaatggt
    36121 tcagcaggtg ttaaaaataa gcttacaaaa gatgaaatag aaaatgcaac atgtataata
    36181 gtggcagcag ataaaaatgt tgagatggct agatttaatg gcaaaaaagt cataaaaact
    36241 aaggtggctg atggtataca caaagcggaa gaacttatag acaaggctgt aaatggagat
    36301 gctccaattt accatggagg agatggaagt cacaacgaat caaatgaaga aagtgaaagt
    36361 ggatttagaa aagtatacaa acacttaatg aatggtgtat ctaatatgtt accatttgtt
    36421 attggtggag ggatacttat agcgatagcc tttttacttg atgattatac tattaaccct
    36481 tcaaactttg gttcaaatac acctatagca gcatttttta aaggaatagg agataaagct
    36541 tttggattta tgttacctgt acttgctgga tatatagcat actctatatc tgatagacca
    36601 gcgtttgttg taggatttgt aggaggtgct ctagcaggtg atggtggttc aggcttttta
    36661 ggtgcactac ttgcaggttt tatagcaggt tatttagtag aagggcttaa gaaaatattt
    36721 agtgtactac cagcttcatt ggaaggaata aaaccagtat tgttatatcc tttacttgga
    36781 acacttttga tgggaattat aatgacattt ttagtaatac ctccagttac agcaattaat
    36841 aatgcaatgg ttggattttt aaatggttta ggtggaactt caaaaatagt tttaggatta
    36901 gtattaggtg gaatgatggc tgtggatatg ggtggacctg taaataaagc agcatatgta
    36961 tttggagttg catcacttga atcagggcag tttgaaataa tggcagcagt aatggctggt
    37021 ggtatggttc cgccacttgc tatagctctt gcaactacat tttttaagaa tagatttact
    37081 aaagaagaaa gagattctgg taaagttaat tatgtaatgg gattatcatt tgttactgaa
    37141 ggagctatac cttttgcagc tggagaccca cttcatgtta taccagcttg tgttggaggt
    37201 tcagcagtgg caggagcact tagtatgttg ttcaacgcag ctcttcgtgc acctcatgga
    37261 ggagtatttg ttataccagt ggttacccat ccatttgcat atatattagc tatagctgta
    37321 ggtgctttgg ttggaatgat gcttttagca ttacttaaaa aaccattaaa tcaagaagta
    37381 tagcaattaa aggatattca agaacttaaa atatgatgtt ttataaaaat taatatattg
    37441 ctgaattatt ttaaaataag gattttatga ggctgttata taacgatttg taattgttat
    37501 cttgacagtc ttttattttt atataagtaa aataagtgtg ttttgcttta tttgtattta
    37561 aattgttttt aagttag
//
LOCUS       DAMJWU010000029        37481 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMJWU010000029 DAMJWU010000000
VERSION     DAMJWU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.029"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            185..853
                     /locus_tag="PC460_001620"
     CDS             185..853
                     /locus_tag="PC460_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600507.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            855..1340
                     /locus_tag="PC460_001621"
     CDS             855..1340
                     /locus_tag="PC460_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2600508.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1401..2522
                     /locus_tag="PC460_001622"
     CDS             1401..2522
                     /locus_tag="PC460_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF2600509.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2803..3993
                     /locus_tag="PC460_001623"
     CDS             2803..3993
                     /locus_tag="PC460_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2600510.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4127..4382
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4441..5442
                     /locus_tag="PC460_001624"
     CDS             4441..5442
                     /locus_tag="PC460_001624"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF2600511.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5546..5935
                     /locus_tag="PC460_001625"
     CDS             5546..5935
                     /locus_tag="PC460_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF2600512.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6096..7304
                     /locus_tag="PC460_001626"
     CDS             6096..7304
                     /locus_tag="PC460_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2600513.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7390..8466)
                     /locus_tag="PC460_001627"
     CDS             complement(7390..8466)
                     /locus_tag="PC460_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2600514.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8695..9222
                     /gene="hpt"
                     /locus_tag="PC460_001628"
     CDS             8695..9222
                     /gene="hpt"
                     /locus_tag="PC460_001628"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2600515.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9311..10243
                     /locus_tag="PC460_001629"
     CDS             9311..10243
                     /locus_tag="PC460_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF2600516.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10305..11531
                     /locus_tag="PC460_001630"
     CDS             10305..11531
                     /locus_tag="PC460_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2600517.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11615..11836
                     /locus_tag="PC460_001631"
     CDS             11615..11836
                     /locus_tag="PC460_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2600518.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            12025..12195
                     /locus_tag="PC460_001632"
     CDS             12025..12195
                     /locus_tag="PC460_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600519.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12288..12749)
                     /locus_tag="PC460_001633"
     CDS             complement(12288..12749)
                     /locus_tag="PC460_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600520.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            12932..13399
                     /locus_tag="PC460_001634"
     CDS             12932..13399
                     /locus_tag="PC460_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600521.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13442..15205
                     /locus_tag="PC460_001635"
     CDS             13442..15205
                     /locus_tag="PC460_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF2600522.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15213..15821
                     /locus_tag="PC460_001636"
     CDS             15213..15821
                     /locus_tag="PC460_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2600523.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15893..16462
                     /locus_tag="PC460_001637"
     CDS             15893..16462
                     /locus_tag="PC460_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2600524.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            16980..20519
                     /gene="nifJ"
                     /locus_tag="PC460_001638"
     CDS             16980..20519
                     /gene="nifJ"
                     /locus_tag="PC460_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF2600525.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(20911..21807)
                     /locus_tag="PC460_001639"
     CDS             complement(20911..21807)
                     /locus_tag="PC460_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF2600526.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PGNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPEDNNNSDSTNKPD
                     DNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYF
                     SHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFS
                     KIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(22303..23637)
                     /locus_tag="PC460_001640"
     CDS             complement(22303..23637)
                     /locus_tag="PC460_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2600527.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            complement(23699..24475)
                     /locus_tag="PC460_001641"
     CDS             complement(23699..24475)
                     /locus_tag="PC460_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2600528.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            complement(24580..25245)
                     /locus_tag="PC460_001642"
     CDS             complement(24580..25245)
                     /locus_tag="PC460_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF2600529.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            complement(25245..25901)
                     /locus_tag="PC460_001643"
     CDS             complement(25245..25901)
                     /locus_tag="PC460_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF2600530.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIIEGGNL
                     "
     gene            complement(25910..27088)
                     /locus_tag="PC460_001644"
     CDS             complement(25910..27088)
                     /locus_tag="PC460_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2600531.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTITVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQSKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            27443..28345
                     /locus_tag="PC460_001645"
     CDS             27443..28345
                     /locus_tag="PC460_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2600532.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            complement(28561..29373)
                     /locus_tag="PC460_001646"
     CDS             complement(28561..29373)
                     /locus_tag="PC460_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600533.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLIDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(29376..30335)
                     /locus_tag="PC460_001647"
     CDS             complement(29376..30335)
                     /locus_tag="PC460_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600534.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            complement(30441..32000)
                     /locus_tag="PC460_001648"
     CDS             complement(30441..32000)
                     /locus_tag="PC460_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2600535.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            32219..33196
                     /locus_tag="PC460_001649"
     CDS             32219..33196
                     /locus_tag="PC460_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600536.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            33197..34168
                     /locus_tag="PC460_001650"
     CDS             33197..34168
                     /locus_tag="PC460_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF2600537.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKKIKSVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            34320..36362
                     /locus_tag="PC460_001651"
     CDS             34320..36362
                     /locus_tag="PC460_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDF2600538.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNIMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVIKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATILLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            36623..37465
                     /locus_tag="PC460_001652"
     CDS             36623..37465
                     /locus_tag="PC460_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2600539.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
BASE COUNT        13983 a         4608 c         6184 g        12706 t
ORIGIN      
        1 aattaataaa aaaatattat atttttaaac ttgaaaagta agtaattata agctatttaa
       61 tcactggaat aaatttacaa taaaaaaata aatacaaagg ctttatcatt aaggtattgg
      121 cataagtttt gcttattaaa caagtatact aataaaattc atttaaacag gagggagaat
      181 ttatatgagt aaattagaat caacaacaga aaaagttgag gtaaaatggt atgggtatgt
      241 agcattaata ttgggagcat tatttttttc aggaatattt aaagatgcac caggaccatt
      301 aaaagtattg gattttaaca atgtattagg ttcttttgga acattgggaa caattaatga
      361 tggtgtaggt acattagctg caaactttag aggtgatgga ggtactggcc ctcgagatgg
      421 atggctatat gcattgacat taataccatc agttatgttt gcacttggag tagttagagt
      481 tatagaccac ttagatggaa tgaaggcagc tcaaaagctt cttagtccac tattaaagcc
      541 attattaggg ttacctggat ttgcaggact aactttgata gctagtttac agtctacaga
      601 tgcagctgca tctatgacta aagaacttaa agatgatggg tatatagatg aaaaacaaaa
      661 agctgtattt tgtgcatttc aattttcagg tgcgtctgca attacgaatt tctttgcttc
      721 tggagcagca ctatttccat ttataggaga tgtaccaata tttataccac ttgctttaat
      781 attaattatg aagtttgttg gggcgaactt acttagatta tatttaaata aatttgacaa
      841 gggggaagct taacatgagt gaggtagcaa aaaaagaagt aaaagttaaa aaaggaaatg
      901 tgatagatgc gtttattgga ggagctagaa atggattcca aataagtaca aattcaatgg
      961 ctccaaatgt catcttcggt tttgctataa tttctgtgtt caatttaaca ggtcttttgg
     1021 atgtaatagg gacaatattt acacctgtaa tgagtatctt tggacttcca ggtgttgcag
     1081 caacagctat aatgacaata tttatatcaa tgggaggagc atctggagta atcgcaggtt
     1141 tatttacagc aggtcaaata agtcaagcac atgttgcaat tctattgcca atgttattgc
     1201 tttcaggagc gcagatacaa tttatgggga gacttttagg aacagctgag ttaaaaacta
     1261 aatattatcc acatatgttt gttatagcaa ctttaaatgg ttgtattgca atgcttataa
     1321 tgaattattt cgtagtatag gtaattataa taaagattta atataaatgg aaagtttaaa
     1381 ttttaaggag gagctagatt atggaaacta acaaagacta tattgagtat gtacataaag
     1441 tgtatgatta tctacacact ataccagaaa aaggatttga agaatataag acatcagagt
     1501 ttgttttaaa agagcttgaa aaaatggggt ttgaaaatat taataaagga attgcaggaa
     1561 ctggtattat agtaactata gattctaaaa aagaaggacc agtatttgct ataagagcag
     1621 atatggatgc attggaattt atcatagatg gagaaaagaa aatgatacat gcttgtggtc
     1681 atgatggtca tacttctatg cttttaacag ctgcaaagtt aataaaagag agaaatttag
     1741 taaaaagagg taagttaaaa atactttttc aaccaggtga agaaaaatta tatggagcat
     1801 taagagtaat tgattctggt ttgttggatg atgtagaaga aatggtaggg atgcatttaa
     1861 gacctggaca agattgtagg ttgggaaagg cgatacctgc aatgtaccat tgtgctagct
     1921 gtataatgga atttacgatt aaaggtatca cttgtcatgg ttctaaacca catttaggag
     1981 taaatgcagt agatgctgtc tgtttagcag tgaatgcaat aaatgcaata agagtaaatc
     2041 cagctatacc aaattctata aaagtaacaa atatatcagt taagggagaa acttataata
     2101 atataccaga tgaagcattt atggctttag atataagggc agaaagtaat gatataatag
     2161 aagaattaaa agaaaaagca atacatgctg taaaaactgc tgtaagttct ataaatgcag
     2221 aagctgtact aatttccaaa gatggagtac cagcagcaga atttgatgaa gaacttgtaa
     2281 acttggctga agaaactata aaagatgtat tgggagctga tgcatctata ggtatattta
     2341 aaaattctgg tggagaagat ttccattatt atacacaaaa actaaaatgt aaaactacat
     2401 atattggatt aggagctgat gctacaccag gatttcataa tccaaatgta acatttgata
     2461 cgaaagcttt agaatatggt gtagatatat ggtgtagatt ggttgaaaag agattgggat
     2521 aacatccaaa ctaataattc tacatttaaa attataatta tagaagttat agaaaaagat
     2581 tgcttatgtt aattagaaaa taattaatat gagcaatctt attttgatat aatgaaactg
     2641 attttggaat attgaaaaaa tatgatggaa aagtaattat attttaaatc tattggacaa
     2701 aaaagtgata ttattataat attaagaatt gtaaaataat accaaagtgc atctagtaat
     2761 agacttaaca attataaaaa ttttattctt aggagggaaa aaatgagtaa gtataatttt
     2821 gacagagtaa tagatagagt aggaacagat tgtgtaaaat gggattttag aactaattgt
     2881 tcaacaaaag cacaaaaaga tggtttgcct ttttggattg ctgatatgga ttttgaatgt
     2941 gcagaaccaa taatagaagc attacgtaaa aaagttgaac ataaaatatt tggatatagt
     3001 tcaaatgata gtgataaata ctttaatgca gtttgtgatt ggtataaaag gagatttaac
     3061 tgggaaataa atagaaaaga tattattttt agtcctggtg ttgtacctgc tgttgctatt
     3121 ttagtaagaa tattaactaa ttctaatgaa ggtgtaatag tccaaaaacc agtgtattac
     3181 ccatttgaag ctaagataaa gagcaataat agggatgttg taaacaatcc tctgatatat
     3241 gagaatggta cttataggat ggattatgat gatttagaag aaaaagctaa gtgtagcaac
     3301 aataaagtac tgatactttg tagtcctcac aaccctgttg gaagagtttg gagagaagat
     3361 gaattaaaaa aggttgtaga aatatgtaaa aaatatgacc tatggataat agctgatgag
     3421 atacattctg atttaattag aaaaggattt aaacatactc ctttacagtc attatgccca
     3481 gagtacaagg ataaaatagt tacatgtata gctccaagta aaacatttaa tctggcagga
     3541 atgcagttgt ctaatataat agtgaataat gatgaactaa aaaagaaata tcaagaagag
     3601 gttacagctg ttggagttgc aacttcacca aatccctttg ctattgtagc tacaatagca
     3661 gcatataatg aaagtgaaga ctggcttaat gaattaaatg attacttaga taataatata
     3721 caatttattg atgaatactt aaaagaaaac ctaccaaaag taaaacttgt ttatccagaa
     3781 ggaacatatc tagcatggtt agattttact gcttatggat taaatgaagt agaacttgaa
     3841 gatttaatgt ttaaaaaagc taatgtatta tttgatgaag gttatatatt tggaaaagaa
     3901 ggaataggat ttgaaagaat aaatgtagct tgtccacagt ctttattaaa agaatgtatg
     3961 gacagattaa agactatatt tgaaaattta taaagtatag gtaaaaattt ataaagtgtt
     4021 taaaaataaa aaaataataa acttgacaaa ttttaactaa tcacttatca tactattatg
     4081 aataaataga aaacataaga gagctaaatt ttattgaatt aaactcttaa agactttgaa
     4141 aggaagagta gttatagaat gtaattaaag cgagttggtg atggtgtgag accaatatga
     4201 agtctataat gaagaacatc ctggagttgc taaccgaaat tttaaaagag taggcttagt
     4261 cggaaccaaa ccgttatcat attggggtgt atcgagtaat atctgtacat tgtgagctgg
     4321 gtttgaattt atttcaaact aatcagggtg gtaccgcgga aaagcaacct ttcgtccctt
     4381 ttatataggg atgaagggtt tttttaatgg aaaaatatat aaatataggg agtgaatgat
     4441 atgagcacaa taataccaaa ggattataaa agtagtttga acgtaattga tacacaaata
     4501 gctataaaaa aattaaaaga tttttttgaa atgagactaa gtaatgaatt aaatcttata
     4561 agagtttctt ctccgttatt tgtattgcca gaaacaggtg caaatgataa tttaaatgga
     4621 gaaaaggctg taagctttga tataccattt atgaataaaa atgcagaaat tgttcaatca
     4681 cttgcaaaat ggaaaagatt agcccttaaa aaatatgggt ttgaagttgg tagtggttta
     4741 tatacagata tgaatgcaat aagaaaaaat gaagaattag acaatataca ttctctttat
     4801 gtagaccaat gggattggga attggttata gataaaagta gtagaaatga aaagactcta
     4861 aaagatgttg taaaaagatt gtatggagta tttaaagata ctgaaatttt tgtatgtagt
     4921 atgtatgaag gtataaaaga aatattacca gaagaaataa cttttataac atcacaagag
     4981 ttagaagata tgtatccaga attatcacca aaggaaagag aagataaaat tgtaaaagag
     5041 aagaaagcag tatttataac tcaaatagga aaaacattaa tatcaggaga aaaacatgat
     5101 ggaagaagcc ctgattatga tgattgggaa ttgaatgggg atatattatt ttacaatcct
     5161 gttttagatt cagcattaga attatcttca atgggaatta gagttgatga agaatctctt
     5221 gatagacaat tgaaacttgc tggatgtgaa gagagaaaag agtttgacta tcataagatg
     5281 cttttaaatg gcgaacttcc gtatacaatt ggtggaggaa ttggtcagtc aagaatatgt
     5341 atgtatttct tacaaaaagc tcatatagga gaagtacaag ttggagtatg gccacaggat
     5401 atgattcaaa attgtagtag tgcaggaatt gaattattat aatttaaata taaactaatt
     5461 gataaaattt atatctaata tgttcatcaa attaattata tcggttataa tgtagaataa
     5521 cctataatta taagaggtga taaatatgga gtttttaaaa ataatcatta atatagtttt
     5581 agatatttta aaaaaaattt tggtcagatt taaaaatgct aaatttggtt tggtttttgt
     5641 atttgattta cttaaactac ctgattttat gacagataaa agaatcaata tagttgataa
     5701 aataaaggta atttctgtat taatttttac tatatcctac tttgtatctg gggttgatat
     5761 aattccagag atgattgcag gggcatttgg ttttatagat gatgctatag ttttaatttg
     5821 gagtataggt atagtgaatg aagaaattaa taagtataga gttatcgcaa aaaaagataa
     5881 acattcaaat ataattgaaa atgtagaatt ttctataaaa gatgaagaag aataacaatt
     5941 tatatatgtt attctttttt ttattaacaa aaaatggtta aattgttgac ggagaggtaa
     6001 aactctatta tcataataag tggatatcca atatttaact aagtattcaa tgtgcaagaa
     6061 tgcttaattt aaaaaagtta tggagggtaa atattatgaa gaaaaaaata ggtttagtgc
     6121 ccaaactaat catcggtatc atagtcggta ttttaatcgg aagttatgca ccagaaataa
     6181 tagtacaaat attagtaaca gttagtactt tattctcagc attcctaaaa tttgttatac
     6241 catttataat tataggtttt gtaacagcag gtatagctga tttagcaaca ggtgcaggaa
     6301 agttattggg gataactacg ggaatagcat atggttctac cttaatagca ggattattat
     6361 catttgcagt atctacatta atcttcccaa attttattga tgcaagtgtt gcaagtcaaa
     6421 ttggagaccc agaggcagga atgttagctc caatattcac aattccatta gagcctatgg
     6481 tagatgtaac agctgcaatc gtatttgcat ttgtaatggg acttggaata tcagctttaa
     6541 gaaatcatgg aaaaggagag actttattta atttcttcca agaatttcaa ggaatcgtaa
     6601 cgaaaacgtt gtcaacagtg attatacctt tattaccttt atacatagca ggaacttttg
     6661 caaatataac tatagcaggg gaagtatgga caatattagg ggtattctgg aaagtatact
     6721 tagtagtaat acctttacat tttgtatata tggcatgtca attcacagca gcaggtgtat
     6781 tttcaggaaa aaatccagta agaatgttaa aaaatcaagt acctggatat ttaacagcta
     6841 taggaactca atcttcagca gcaactatac cagttaatgt tggttgtgca gaaaaaaatg
     6901 gagtaagtaa gcaaataaga gaatttgttg tacctctttg tgcaacaata catttatcag
     6961 gtagtatgat atcaataaca ggatttacaa ctgcagtttt aatgatgaat ggtatgccac
     7021 atggaatagg tgtattattc ccttatattg caatgttagg tatagcaatg gttgcagcac
     7081 caggagcacc aggtggggct ataatgagtg cacttccttt cctaccaatg gttggaatac
     7141 catcagatgg tgggcttgca agtttaatga tagctttata cttaactcaa gacagttttg
     7201 gtacagcatg taatgtatca ggagataatg caatagcagt agtagtagat aaaataaatg
     7261 atggaatgag taaaaaagga aataaagaag agcaaagtgc ttagtttttt aataatcaaa
     7321 aatgagttat ctcaaataga gatagctcat tttttatggt ataaaaaatt ataaaataaa
     7381 gtgatttttt tatttaaaaa ttgctttaag agatgatatt aataatttat tttcattagg
     7441 ttttaatata catactctaa agaaatactc atctagacct tcaaatgaac aacaatctct
     7501 tattattatt ttttgtggaa gcaattgttc tcgaagagat ttagcagtca attcctttgt
     7561 cttgatttta cacaatatga aattgccttt agtattgtat atatctaagc tttttatatt
     7621 cttaagctct ttaagcaggt aatctctttc agtgttcatt aaggaaatag tatttgatat
     7681 aaaatctaaa tcactaaaca tgatttcacc cattttagaa gctattatgt ttatattcca
     7741 taaatctaga tttttattta tttcattttt gacattagtg ttagatataa gaccatatcc
     7801 aagccttatt ccaggcgttg aaaaaaattt tgatgtacct cttataacga ataaattaga
     7861 ataatcatca actaattgag tacaagagta tgtatctgta tcagtaaact ctacataagt
     7921 ctcatcaatc attaaaaatg agtctgtatt ttttaaaatt tctctcactt ctactttagt
     7981 aaatgcaaat ccagttggat tactaggatt acagattact accaaatcat aatctttagc
     8041 attgatagtt tttataagat tttctaaatt tatatgaaaa ttatcttctt cttttgcaaa
     8101 gtatttttct atagaacaat taattttaga taactctttt tcatactctg aataggctgg
     8161 agagagtaaa agagcctgtt tagggttaat tgtatgtata aatgatgaaa ttagttctgt
     8221 agctccactg ccaagtacta tattatctat agaacatttg cagtaattgg atatagaagt
     8281 ttttaagtct atgtaatcag ggtcaggata catagatacc atatctatat tgtttacaat
     8341 gtattgtttg gctaaacttg atgtaccaaa aggatttata tttgagctaa aatccataaa
     8401 ttcttccttt gaaaagccat acttactaga taaactatat aaattagctc catggtttgt
     8461 gctcataaaa gtacctccaa aaataaaatt atataatcga taaaaataac tgattaatat
     8521 atgtacatat agattataat acaaaggaat cgttattcca aatttttaat ggaaaaagca
     8581 aaaaagtatg gataagctgt taatttaggt ttaaaaatga tgaaaaagat tttaattgat
     8641 attttggtat gctataatat ataagttaat agaaggaaag cgggaggata caaaatgtac
     8701 aaagttacag gaaaaatgtt gacagaagaa caaataaaag aaaaagttta tgaattagga
     8761 aaaaagatag aagaagactt taaaggcgaa gatttactag tggtaggtat acttaaagga
     8821 gctagtgtat ttgtttcaga tttaataaga tgtatagatt tggatgtaaa tatagatttt
     8881 atgagtgtta caagttatgg aaactcaaca gagtcttctg gaacagttaa aatattaaaa
     8941 gatttagatg tagatattga aggaaagaat gtgttgattg tagaggatat aatagattct
     9001 ggtttaacat taagtaatct agttgcagct ttaaaaacta gaaatccaaa atctttaaaa
     9061 ctatgtactc tacttgacaa acctcagaga agaaaagcta atatacctgt agactatgtt
     9121 ggatttgtta tagaagacaa atttattgtt ggttatggaa tagactatgc tgaaaagtat
     9181 agaaatttac cttatatagg tatagttgag gatgtagaat agatttaaat gtgaatcaaa
     9241 agcactaatt tctaagggag ttagtgcttt ttagttaatt atataaaatt ttagtaaagg
     9301 agacatctat atggatattc agttaaatat attattattt ttccaaaatt taagaaatcc
     9361 aattttgaac tttatatttc ttgtctttac aataagcaca gaagcaccgt tattaatatt
     9421 aattacaaca attatatact ggtgtataaa taaaaaatgt ggacagaaaa tactgtttgc
     9481 tataattgga aattttgttg taaatttagg catcaaagag tttgtaaaag ctcccagacc
     9541 aataggaata aaaggtcttg aatcattgag agtttctact gctggtggtt attcttttcc
     9601 aagtgctcat actcaaactg caacaacatt ttgggttagt attatgacta tatttaaaaa
     9661 gagatatctt catttaatag gtgcagttat ggttttagga gtaggattgt ctaggctgta
     9721 tttagctgtt cattggccaa tagatgtgat agcgggttgg atattaggaa tattttttac
     9781 agttgtattt gtaaaaatat ttgactatat tgatgataat aaaaattata ttttacttgt
     9841 agttttactt ataccattta taatagtggc tatatttcta aatagccctg aatattttga
     9901 aaaatttggt ataatagtag gatttgtatt tggatatatg gttgaggata gatttgttaa
     9961 atttaataca gataataata acaaaaaaat aaattttagt aataaaaata aggggagtaa
    10021 aaatataatt ttttcaagaa tatgtaggtt tttggttggg atagtaacaa taggaatgtt
    10081 atatattggc attaagttgt ttatttcaat gttaatagtc actttaaata taagtgactc
    10141 tactatgagt ataattttta tgaattttat taagaataca gtagttgttt tttatggaat
    10201 agcaggagct ccagcattat ttaaactttt aaaattaaat tgattttaag gtatagagtc
    10261 aatagtttaa attaaaaaaa ttaagggtag aaaagagtga ttatttgaag aaggtagtaa
    10321 taataggtgg tggaccagca gggatgatag ccgcatcaac tgcatgtgag aaaggctatg
    10381 atgtaacatt aattgagaaa aatcataaat taggaaaaaa acttgcaata acaggaaaag
    10441 gtagatgtaa tattactaac gcttgtgaaa tagaggaatt aatagaaaat gtacctacaa
    10501 atggaaaatt tctttatagt gctttttata cttttacaaa tgatgatgta ataagtatgt
    10561 ttaataatct tggagtaaag actaaaacag aaagaggaaa aagggttttt ccagagagtg
    10621 ataaagcatt tgatattgta aatgctcttg aaagacaact taaaagtaag aaagtaaata
    10681 tattattaaa ttcgaaagta gaaaaaataa tatctaaaaa taataaaatt gaaaaagtaa
    10741 ttttaaacga taaaaaagaa attaagtgtg attcagttgt agtagcaact ggtggattaa
    10801 gttatccact tactggttct actggtgatg gatataaatt tgcaatttca caggggcaca
    10861 caattataga tacaaaacca tctttaattg gtatagaagt acaagaaagt tttacaaaag
    10921 atttagaaaa attatcattg agaaatgttg aaataagggt gtttaatagc aaacaaaaga
    10981 aagtttatag tgattttgga gaacttgaat ttactaaatt tggtttagat ggacctataa
    11041 tcaagtctgc aagttgtaga atgaaagata caaggaaaga aaattacact atattattag
    11101 atttaaaacc tgcattagat gaggaaaaat tggataaaag agttcaaaaa gattttcaga
    11161 agtatacaaa taaaaaattt gaaaaagcat tagatgattt acttccaaaa aaactaatac
    11221 ctataataat aaacttaagt gaaattaatc caaatacggt tgttcaccaa atttctagag
    11281 agcagagaaa aaacttagtc catttattaa aaaatttaaa gtttacagta aagagataca
    11341 gacctattga agaagctata ataacttctg gtggtgtaaa agtaaacgaa ataaattcaa
    11401 gtacaatgga atcaaaatta gtagaaggtt tattttttgc tggagaagta atagatatag
    11461 atgcatatac aggtggattc aatttgcaaa tagctttttc tactggatat ttggcaggtt
    11521 ttaattgcta aaaattacct gaattaaaaa tgtttaaaat cacataatat tatcagcaac
    11581 acaaaataaa ttaaacagaa ggaaggtaat caacatggca agtaacaata acaacaacag
    11641 aacagtagta ccaggagcaa aagaggcttt aaaccaaatg aaattagaaa tagctaatga
    11701 acttggtatg agtaattatc aacaagttga taaaggaaac cttactgcaa gagaaaatgg
    11761 ttatgtaggt ggatacatga ctaaaaaatt agttgaaatg gctgaacaac aaatggctgg
    11821 aaaaagcaac agatagttat aaataattat ttataactat tggctattag tttagctaga
    11881 gctatctcaa atatagaaag taatttctaa attgagatag ctttttttta ttaaattatt
    11941 aaatagatat atttttatgt aataaatttt atgtatttgg ataaaataat acaaatgaat
    12001 atgttacata aggagtgatg taaaatgagt agtgtaaatt ttgaggattt aaaaaacaaa
    12061 tttattaata gtgacttaga tgaaaaaata agaatatata caacaacaga aggtttatca
    12121 gtagaacaat ttaaagagct actaaaatat tatccaatac aacatttatc taaactagaa
    12181 aaagcacttg gatagcagta tttaatccaa aattaaaaag gcaatttatt taatattaaa
    12241 aatatttttt attaaataaa ttgccttttt ataataggtt taaattatta tgcttttgat
    12301 atataattat agattctctc atttccaagt tcaatagacc cagtattaat aagagtaact
    12361 attccttcta tatctctagc agttaagtat ttatatggaa ttaataaatt ataaccagta
    12421 ttacttagta agttttttaa ttgggttatt tgagagatat tgaaacattt tgcagtttta
    12481 gtagattttt ttcttaagtt agataaattt tcattttgaa tatttattaa agtttctata
    12541 tagtttctat aagatatctt aaagtttgtc tcattgacta ttttttcatt aagtatacta
    12601 ctgataaaat cttgagacaa tatattatct gcaaatttat ctgctgttaa ttttccagat
    12661 gttatgcctt tttgatattt tctaagaatt tttttagtat agcagtatat tttatttctc
    12721 gtttctaaat cgagttgttc tatttgcaca aaaccacttc cttctaatta taaaaatatt
    12781 tattaaaaat ctatacttaa ctaataaagc ttcaataact tatttacaat aaatactaaa
    12841 tcataaaata tttgttattt tttattatta ccaagtataa aaaatattat aattgtaaaa
    12901 cttacaaata tatgtaaaat atttaggagt gatgaaaatg ttaaatggga taataaagct
    12961 tttttattct gaaattgtaa aaacaaagat taataactta gattcaagtg gatattcttt
    13021 aagagatgaa tgtaaagaaa tcagaaatat aactctaaag atagatagtg tattttttaa
    13081 gcttttaaag ttagcttttg gttcaatatt gcttatggta gcttttaatt taaacatggt
    13141 tttaggtata ggagtgtttt tagttggtgt agtttatata ctatataaaa gaaatatgga
    13201 agaacaagta aaagagcata taaataatgt gaaaaataat atcgacatct ctaaaataac
    13261 ttttatggat gaaaaaggga gaacaggtct aaatgcatta attacattac ttcttatagg
    13321 attattgaca ggatttaact ggatattagt tgctagtttt gtcgttgttt ttgtttttac
    13381 tataaagaat gtatgttaaa atactaataa atagtttaat atataggggg agaaattgag
    13441 tatgactaat ggcagttata aaatgaagaa tttagtggaa atatccgcta tggtgactga
    13501 atctacagat ttttttaata taaaagatga cataatagaa aaaatgttag aagttgttca
    13561 ccctacaaag gcatgtgtga atctatttta taaaaatgat tctaaatatg cctacttagt
    13621 atgttcgcaa accttagaat atgtacctca aatttttcca ataaatagtc ttaaaggtgc
    13681 aaaaattgat tttgacacat atccagagta tatacatgag gctgtaaatg aaaaaaaaat
    13741 aatatatgtg gaaaatgtat ttgaagattc tagagcagaa ggtgaacgag atttagcaaa
    13801 agctgaaggt tatattggga gaatagtttt tccttttata ataaataacg tagttgttgg
    13861 atttatgact tgttttttaa aagaggatga ttatataaca gaacaagata tagattttat
    13921 atcatctgtt gcatcactct taagtttatc aatagaaata actaataaaa ataataatgt
    13981 acaaatttta attgataaac taagaggctc aataagtgcc ataaatgaag ctactaaaaa
    14041 actatacctt aataagagta ttgatggatt tttggaaaat ttaagtaaac aagcgtgtaa
    14101 tattactaat agtaaagaag ctttaataat tataaatagt catcataata aggatgctat
    14161 agttagttgt tataatgttg agcaaaaaaa gaaaacaaat ttatatccta tgattgatat
    14221 gtttttaaag aaagagtcat tgggggggta tttaaataat ttaaaaccat gctcaaaaaa
    14281 gggaataaat ttggaaagtt atatatatta caaacttcag gataaaaatg agactatagg
    14341 atgtatagtt tgtgcaaata gcaaaaacta tactagtgat gatttaaata ttcttagtat
    14401 attgtccaaa caagtttctg ttggtatgca attatatgaa cataatgaaa aggaagtaaa
    14461 acacaaagtt cttgaaaacg agttgaacat attaaataaa caacaacagc ttattatgga
    14521 taaaggtaaa atggattgta atgatgagaa agaactttat ttttaccata aaccagcaag
    14581 ggtagtaggt ggagattttt attatgctac aaaagtggat gatgaacata tagtttatat
    14641 tgttgctgat gtaatgggac atggaatggt atcaaattat gttgttgcaa tgataaaagg
    14701 tgcttttaaa gtattatgca atcaatacaa tacaccaaga gaaataatga caaatttaaa
    14761 taggatgtta tatgatgaat ttgacaagat gggagtattt acaacttgtt tgataggtat
    14821 tatagatact aaagaaaata tgattactgt gtctaatgca ggtcactata gtcctgtagt
    14881 tgtaaagaaa gatggcacaa tagaaaataa tttaaattgt aaaaagggca tacctattgg
    14941 aataatggaa gatgctactt atgaaaataa tactctttca atggaagatt atgcaatggt
    15001 atgtatgtac actgatggaa ttttagagat aaaaaattca tcaaaagaag agtatggtat
    15061 aaacagatta gaaaactttc taaaagaaaa ttttagatta aatcaacagt taatagtaga
    15121 aaatctaaaa ttggatttaa agaacttctc atcaaaagat aattatgatg atgatatttt
    15181 aatagttatg ttaaaggata ggtagattga ttatggattc aaacgataaa aaaggactta
    15241 aaagtccaga gattataata ataaatggaa gtccaagaat taataaaaat tgcagtttta
    15301 tagcaaatga agtaaaagct atgctagaag agtgtggatt aagttgtaaa atatttaata
    15361 tctacaatat ggatattgaa tattgcacag catgtggatt ttgtgaaaaa acagggtatt
    15421 gcaagtttaa tgatgatatg aagccattat ataaaatgtt tgatgattca aaaggaacta
    15481 tagtaataag tcctgtacat tttgattcaa tatcagctaa attaaagact gttgttgata
    15541 gaactcaagc tatatatgca agtaaatatg tattaaataa accttcaatt gatagaagta
    15601 agaaacggat aggaatgtat attgcagtgg gagggagtaa atcatatgat tcacagttta
    15661 aaggtggaca gattgttatg gattttttct ttaagagtat aaatactaaa cttttacaca
    15721 atatatattt aaacaataca gatgatgtat cttatataga taatcaagaa tttaaagata
    15781 taatatataa aaatttaaag atatatattg attcaatata atttgatttt gtaggtaata
    15841 ttatgtataa ttgtattgca ataatttaaa taattaaaag gagggaattt caatgaaatt
    15901 accaatagca ttatctaata aacatgttca tttgagtcaa gctgatatag acgtattatt
    15961 tggagcaggt catgagttaa ctaaatttaa accattatct caaccagggc aatatgcctg
    16021 tgaagaaaaa gtggatttag taggaccaaa aggaacttta aaaggagtta gagtactagg
    16081 accagctaga gctcatactc aagttgaaat atctttagca gatggtttcc cattaggagt
    16141 aaaagcacca ataaaagaat ctggtgatat agctggaact ccaggtgtta aattagtagg
    16201 acctgctgga gaagttgaaa tgaaagaagg agttatagtt gcttctagac atatacatat
    16261 gagcaatgaa gatgctgcta aatttggtgt aactgataag caaatagtaa aagttaaaac
    16321 atctggacca agagcattag tatttgaaaa tgtattagta agagctagtg acagctttaa
    16381 tctagaaatg catgttgata tggaagaggg aaatgcagca ggcgttaaaa atggagattt
    16441 agtagaatta atagttgatt aatattataa atagacttta tgctagtaga tattttattt
    16501 gctagcatat ttttaaatgt aataaatttc tataggataa atttaatcaa ttagattaat
    16561 ttataaaata aaggggtatt ataaaaaatg taataaaaac ttacaaaaat atgatgtaat
    16621 atggtaaaat tatatatata aaaatttaca tgtaataagg ataagcattt tactttttat
    16681 actaatatga acaaatagat aaaaaagttt gaaaaatcta acttaattta ttatatattg
    16741 tatacaaaat tatgaaaaaa acttaacata tttaatttta aaaaaaatct acttaaatat
    16801 gtaattaatt tacaattttc aaaggaattt tatctaatct gtagtattat attagaagat
    16861 agagtatttg cgcgactata tgtgtgttta tagttttgtt gtgtaaatta tatgtaaata
    16921 taaaaaaata gacttgttat aaacaagtac aattgtagct atcaaggagg atacatatta
    16981 tggctaagtt tatgaaaaca cttgatggaa atacagctgc agctcatgtt gcctatgcgt
    17041 ttacagatgt agcagctata tatccaatca caccatcttc aactatggct gaagtagttg
    17101 atgagtgggc atcacaagga agaaaaaata tatttggaca aacagttaat gttgttgaaa
    17161 tgcagtcaga agcaggtgca tcaggaacat tccacggttc tttacaagga ggagctttaa
    17221 cttctacttt tacagcatct caaggtttat tattaatgat acctaacatg tataaagttg
    17281 caggagagtt gttaccagga gtattccatg taagtgctcg tgctttagca tctcaagctt
    17341 tatcaatatt tggggatcac caagacgtta tggctgctag acaaactgga tgtgtattat
    17401 tagcttctgg ttcagttcaa gaagtagctg atatagcacc tgttgcacat ttagctgcaa
    17461 tagaaggtag attaccattt atacatttct ttgatggatt cagaacttca catgaaatcc
    17521 aaaaagttga attactagaa aatgaagatt atgcaagttt attaaacttt gaagcagttc
    17581 aagcatttag agataatgct ttatctccaa atcatccagt tgctcgtggt actgctcaaa
    17641 acccagatat atatttccaa actagagaag cttctaataa atactatcaa aatatagtag
    17701 gtatagttga aaaatacatg gaaaaaatga gtaatttaac aggtagaaaa catagtttat
    17761 ttgattacta tggagcagaa gatgctaaat acgtattaat agctatgggt tcagtaacag
    17821 aaacaataga agaaactata gattacttaa atgctaaggg agaaaaatat ggtttagtta
    17881 aagttcattt atatagacca ttctcaatga aacatttctt agatgctatg ccatctactg
    17941 ttgaaagaat atgtgtactt gatagaacta aagagccagg ttcaactggt gaaccattat
    18001 acttagatgt acgtgatgta ttctatggaa aagaaaatgc tcctatgata ataggtggaa
    18061 gatacggatt aggttcaaaa gatactactc cttcagatat aaaaactgtt tttgataact
    18121 tagtttcaga acaacctaaa aatggattta cagtaggtat agttgatgat gtaactaata
    18181 cttctttaac tccatctgag ccaataaaaa tagcttctaa aggaactata agatgtaagt
    18241 tctggggatt aggttctgac ggtactgttg gagctaataa acaagctatc aaaataatcg
    18301 gtgaccatac tgaaaaatat gctcaagctt actttgatta tgactctaaa aaatctggtg
    18361 gtataactat gtctcactta agatttggtg atactccaat aagagctact tatttaatag
    18421 atgaagctga ttatattgcc tgtcataaac aatcatatgt ttatcaatat gatttattaa
    18481 aaggtcttaa aaaaggtggt acatttgtac ttaacactat atgggaccaa gcaggacttg
    18541 aagaacattt accagctcat atgaaacaat atatagctaa aaatgatata aaattctata
    18601 cagttaacgc tgttaaacta ggacaagaaa taggtcttgg aaacagaata aacatgataa
    18661 tgcaatctgc attctttaaa ttagctgaaa taataccaga agaagatgct gttaaatact
    18721 taaaagattc tatagttaaa gcttatggta aaaaaggtga aaaaatagtt aacatgaact
    18781 atgctgctgt tgatgcagga ataaatgctt tagttaaagt tgaagttcca gcttcttggg
    18841 aaaatgctgt tgatgcagat aaaggagaag ttaaagaacc agaatttata aaaaatatac
    18901 taagaccaat gactgcacaa gaaggaaaca acttaccagt aagtacattc aatggtatag
    18961 aagatggaac attcccttgt ggtactgcag cttatgaaaa acgtggtata gctgttgatg
    19021 ttcctgaatg gataatagat aactgtattc aatgtaacca atgttctttt atatgtcctc
    19081 attcatgtat aagaccagta ttagttactg aagaagaatt agctaatgct ccagaaggat
    19141 ttgaagctaa aaaagcatta ggtaaaggat tagaaggatt aaaatataga atgcaactta
    19201 gtccacttga ctgtacagga tgtggaaact gtgctgacat atgtccagct aaagaaaaag
    19261 ctttagttat gaagccaata gatactcaac ttgatacaga gttagataat tgggcatttg
    19321 cagtaaatcc tgatgaggta gctccaaaag gtgatgttat gcctgcaaac actgttaaag
    19381 gaagtcaatt tagacaacca ttaatggagt tttcaggagc atgtgctgga tgtggagaaa
    19441 ctgcatacat taaagtagtt acacaattat atggagatag aatgatgata gctaatgcta
    19501 ctggatgttc ttctatatgg ggagcttctg caccatcaat tccttatact tgtaaccatg
    19561 aaggtaaagg tccatcttgg gcaaactctt tattcgagga taatgctgaa tatggatttg
    19621 gtatgtacac agctgttaag caaataagaa ataaaatagt agatgctatg acagagttag
    19681 tttctatgga tatctgtgaa gatgctaaag cggtatttac agagtggtta gattctagaa
    19741 atgatggaga agcttctaaa gtagcaagtg ctaaggttgt tgaattatta gaaaaaccag
    19801 cttgtgattg tacagatgaa aaagctaaag agttagtaaa agctataaaa gatagaaaag
    19861 attatctagt taagagatct caatggatac taggtggaga cggttgggca tatgatatcg
    19921 gttacggtgg attagaccat gttcttgcat ctggtgaaga tgtaaacgta cttgtatttg
    19981 atacagaagt atattcaaat acaggaggtc aagcttctaa agctactcca gctgctgcta
    20041 tggctaagtt tgctgcttca ggtaaaaaat ctagaaagaa agatttagga atgatggcta
    20101 tgacatatgg aaacgtatat gtagctcaag ttgctatggg tgctgataaa aaccaattta
    20161 tcaaagccgt tatagaagct gaaaaacatg atgggccatc tttaataata gcttatgctc
    20221 catgtataaa ccatggatta aaagaaggta tgggaagaac tgtagctaac gaagcacaag
    20281 ctgttgcttg cggatactgg catttataca gattcaatcc agaagtaaaa gctgaaggta
    20341 aaaacccatt cactttagat tcaaaagaac caactgcaag cttcaaagat ttcatacttg
    20401 cacaagtaag atattctgct atagctaaac aattcccaga ggaagctgat ggattatttg
    20461 ctaaagctga agctgatgct aaagaaagat atgaaggata caaaaaatta gctgaataat
    20521 attaaagtat tataatgctt atatagaatt gataaaagag agtgtctcaa agtgagcttt
    20581 ttagttcatg aggcactctt tttgtatgaa atcaaatata ttttaatcat ttcaacaatg
    20641 aaaaaggcct tatctctatt ttgagacagc ctttttttat gaagatatta agatcctgtt
    20701 tttacctatt aaatatcttt ttaagttttc cattttcatc ttgccaatac tttttatata
    20761 ttgctctaca cactaaaatt aagagaacaa ataatattta tagtatgtat atttggattt
    20821 gataatttta aaatagatac aaaagagtat ctcatttgtg ttttttatca cttagagata
    20881 atcttttgat atgtatggaa aaagattatt ttatcctaca aagatttgtg tccaataaat
    20941 tgaaccacca cttttttgtg ctactccaac acctatttta ctgaaatttg gattcattat
    21001 attttttctg tgaccttcag aattcatcca tgcatttaca acttctttag gagttttttg
    21061 acccatagct atattttcac ctgctgtctt atagcttatt ccaaattttt tcaacatgtc
    21121 aaatggagaa ccataagttg gagaattgtg tgaaaaatag ttgttatcaa tcatatcttg
    21181 agattttttt gtagcaacat ttgatataga agaatcaaga gtaagaggat ttaatccagc
    21241 tttagctctt tctatattta ctaagtcaac aacttctttt tggtaagctg aaaaactttc
    21301 acttgtagag cctgagttgt tattatcatc aggtttgtta gtagagtctg aattattgtt
    21361 gtcttcaggt ttatcagtag agcctgaatt attgttgtct tcaggtttat tagactcagg
    21421 tttattatta tcaggtttat tagtagagcc tgaattattg ttgtcttcag gtttattaga
    21481 ctcaggttta ttgttaccag gcttattagt agagtctgaa ttattgttgt cctcaggttt
    21541 attagtgttg ttgcaattct tgtcattatg attcttatcc ttacaagagt tatctttatc
    21601 aggcttaagt tctggtttta cagaacaatt tggttttgca gaaaacttgt atttagtatt
    21661 actatatact aaacttttgt tacttatatt tacattattt tcaagtgatg aagcacttgc
    21721 agtaacagca cttgaagctg ttgctaaagt taacactcct aaagctaata tagtttttaa
    21781 ttttgttggt atatttcttc tcatcattaa acacctctta atctaaaaat tctaaaaatg
    21841 ttactgattt gtaaccttta gtgtaggggg ttacccgtga atctatacta acataagatt
    21901 aagacttttt ttactgtaaa gatatgtaaa aaagatatat atggaataaa taggtatatt
    21961 tcagtgtgtt tgacaatttg tattaaaagc ctgtatagta tggaattact tagttagaga
    22021 aaataatatt tttataataa ttccataacg taaatttata gttatgataa attttttttt
    22081 atttggttaa atttattcag tagatgcttt cgtattagca acaaattata attatttaaa
    22141 tctttaagaa ataaaaaggg tctgtctcaa aatagagata aggccttttt tattgtttaa
    22201 atgataggca tatgttattt catataaaaa gagtgtctca ttaagtaaaa agttcacttt
    22261 gagacaccac ctttcatatt atgaatttta atttgtttta tattaataca ttagtaatcc
    22321 taatgtaatt acaataattt ctgctattag tgaaccaact gcaactacaa tgaaagtttc
    22381 tttaaatcca gttttatgtg ttaacttaga taagcttaag aatgtaagaa aaacaccact
    22441 ttgtggaact atagttagta tatttgaagc tattgtagca acacggtgaa ttaattctgg
    22501 atttagacca gtacttaaat aataatctgc aaagtttggc attactattc ctaatgcacc
    22561 agaagaggac cctgttattg ctgacattgt tgaagtcaaa acagtaagac ttataagtgg
    22621 actacctggt attgacatta taagtttgct aaatacatca aaaccaggtg cagccattat
    22681 tactgctcca aatgcaactg cagaagatgt agcaaatata ggacctatag aaccactagc
    22741 acctatgctc aatgtctgta tcttagatga tagaaattta tggaataaaa aagcagataa
    22801 aagaattgca actattaaag ctatgtaaat tacatttttt ttcataaatt ctgaaccaaa
    22861 agtacttcca gttatagcta taattacgag tgctaataga ggaaaaatac ttgcaaaaaa
    22921 tcctggtaat tctctatcta aagattcttc ttctaattct gaagtataag ttgcataagt
    22981 ttcacctttt ctaatacttt tgtttagagc atatttcata tagacaagtc caaacgtagc
    23041 acaacctata ctacctacaa tacttggtat agtagctgca gtaagagacg tatttagata
    23101 ttgaataggt ataacatttt gaatggctgg tgttccagga attatagtca tagtaattgt
    23161 tgcaatacca agccaaagtg gtatctgaat aaggttccaa gaaatatcaa gttttttaaa
    23221 tagagttctt gcaagtggaa tgaccgcaaa cataactaca aataagctta taccaccata
    23281 cgttaatata aaacttacta taaatatagc aactaaaact ctataaggat gattagagcc
    23341 aaatttattt aaaataaaat cagctattga tacagtagct ccactttctt ccataaattt
    23401 agcaagtatt gagcctaata agaatattgc aaaataactc attatatagt ttccaagtgc
    23461 acccatataa ttgtttttgt cagcacctag cattgatgtt acaacatcca ttttatttaa
    23521 aataacaact attaaagttg ctattggagc agctatagtt atatgaattt ctttaactga
    23581 aaaatatata atagctatga taccaataat tataccaatt ggtgcgataa tactcataaa
    23641 tatccctcct tagtagaagg acctgaagaa tactttcttc aagccctaaa tataataact
    23701 attgtgcagt atatcctcca tctagtaagc aagcttgtcc tgtaataccc ttagcattat
    23761 cacttgctaa gaacatagca tagtcggcaa tttcttgaac atctaatagg cgtttttgtg
    23821 gtacaagtgg gtataaaact tcctctaaga cattttccaa tggtatatta cgagtttttg
    23881 aaaggtcatt gaactgacct ctgacaagtg gagtatctac atatccaggg cataaagcat
    23941 taactgttat accagaatcg gcagcttcaa gagcagcaac ttttgttagt ccaataagtc
    24001 catgttttgc tgagttataa gcagcctttc cagcaaatcc tattacacca ttgattgagg
    24061 ccatatttat tatacgacca aagccttgtt ttttcattat aggaaatgct tttttagtag
    24121 ctataaaagg tgctgtcagc attattttta tcataaattc aaatttatca gttgggaaat
    24181 cttctatcat agaaacatgt tgaagtccag cattgtttat taatatatct aatctgccat
    24241 acttttcaac agttttgtct atagcgtggt taatttcttc ttccttagtt acatcacact
    24301 ttacactcat gcaatcataa cctttttttt gtaactctgt tatggtatta tttaaagctt
    24361 cttgatttat atcagagaac attactttag caccattttt taagaacgat tcagctattt
    24421 gtttacctat tccacttgca gctcctgtta cgaatacaac tttatcttta accattttca
    24481 tttcctccaa ttttatttta tttttatatt aatattttaa gcaatacaaa tttatatgta
    24541 atacgtctaa gagattttta ttttctagtt ttaattattt taaacaacag atatttttcc
    24601 atttaatgaa tctgacaata ttaaaggagc ctcagtagca gaaataacat catctaatgt
    24661 gaaagctgga tttatttctt taagtaaaat tccatctgat gttacttcca taactcccat
    24721 ttcagtaact atcaaatcta cagcatttgt tgcagtaagt ggaagagtac aattatttaa
    24781 tatttttgca gaaccttttg aagtatgttc cattgctaca ataacttttt tagctccagt
    24841 tactaggtcc atagctccac ccatacctgg aaccatctta cctggaatca tatagtttgc
    24901 tatattacca tatttgtcaa cctgaagtgc gccaagaaca gttatatcaa catgacctcc
    24961 tctgattatt ccaaaagaag tagcactatc aaagaaacat cctccaggaa gtatagtaac
    25021 tggttgacca cctgcattta caatagtttc atcacttgaa tttccatctt cggctgaacc
    25081 taaacctaaa aagccatttt cagattgtaa gataacgttt acattatccg gaatataatt
    25141 tgctactttg gttggtagac ctattccaag gtttacaacg gctccgtctt ttaattcttt
    25201 agatactcta tttgcaatat attcttgcat ttctaattta tccattataa attacctcct
    25261 tcaatgatgt aattcacaaa aatacctggt gtattaactt catttgggtc aatatcacca
    25321 atttcaacga tttcatctgc ttcaactatt gtaattttgg cagcagatgc cattaaatta
    25381 ttaaaattgt tcatagagcc tttataaact agattacctg ctttatcgac ctttgatgca
    25441 aatagaagag caacatctgc ataaattggt ttttcaagaa ggtattcttt gctatcgaca
    25501 acaatttttt gctttccctc ctcaacaaga gtaccaatac cagtcggtgt gagtatacct
    25561 cctagaccat aaccagctgc acgtatttgt tcagcaagag ttccctgagg aactaactca
    25621 acatctgttt cattgttatt catttgtctt ccagtttctt tatttaatcc tacatgagaa
    25681 gcaattatct ttttaaattg cttatttaca accattttgc caactccttt gtcaataaag
    25741 ccagtatcat tgcaaattag tgtcaaatct tttatatttt tactacataa ataatcaatt
    25801 aaattttctg gtgaaccatt ggccatgaat ccaccaacca ttattgtatc tccatctttt
    25861 acatgagata gagcttcatc aatgctaact attttattca tatttcctcc tatcgtgaaa
    25921 ccaccattga tattccttga ccaccaccaa tacaaagtgt tgcaagtcca gtttcaactt
    25981 tacgtttttg catttcataa attaaggtaa caagtattct agctccactt gctcctattg
    26041 gatgtcctaa tgctattgca ccaccattaa catttaactt tgatgaatct atttgaagtt
    26101 catttttaac agctaatgct tgggcagcaa acgcttcatt tgcttctatt aagtctatat
    26161 cattgatgct aagtccagct tttttcagtg cttttctagt tgcagggatt ggaccagtac
    26221 ccatgacttc aggttcaaca ccagctgaag cataggattt tattttagca agtggtctaa
    26281 tacctagttc gtctgctttt tgttggctca ttagtattaa catagccgca ccatcattaa
    26341 tacctgaggc atttccagct gttacagtac catctttttt gaatgcaggt ttaagtttag
    26401 ctaaaccttc aaatgtcata cctaatttag gatattcatc tttatcaata gtttctattt
    26461 ttcctcgacg tattaaaaca tctactggaa cgatttcttc tttaaagcgg ttgtttttta
    26521 tagcattctc agccttactt tgactttcta atgccaattt atcttgcatt tctctagtaa
    26581 attcaaattt agttgcaata ttttctgcag ttattcccat atgatactga ttgaaagcat
    26641 caataaggcc atctgttagc atactatcta ccatggttat gtcacccatt ttactaccaa
    26701 agcgagcagt tggaacgata taaggagctt ggctcatatt ttcagtacca cctgcaatta
    26761 ccacatcatt ttcaccagat gtgattgatt gggcagctag ctggacactt ttaagccctg
    26821 aaccacataa tttatttact gtgtagcttg gaacagaatt tggtatacct gcatttatag
    26881 caatttgtct agcaacattc tgtccaagcc ctgtttgaag aacatttcca attattactt
    26941 catctatttc agataaatta agtcctacac gtgatatcgc ttctttaaca gtgatagttc
    27001 caagttgaac agctgaagta tttttaaata cacctccaaa acttcctata ggtgttctga
    27061 ctgctgatac gattactaca tctttcatat ctaatagtta cctttcttga atgataatta
    27121 aaaattttct gaataaagaa attaggaaac actataataa agtacttttt taatacaagc
    27181 ttgtttttaa gtatttcaat tacttttatt cctaatgctt taggttttcc tgtttctaat
    27241 tatagattac ggcgaaaaaa gcgttttgtc taagtctaat tttttatata gtgtataaga
    27301 aaaatttata ggataaaaat ttatagtaga tttagaaaaa tattttgtta aaatattaat
    27361 gaaattatgt tgattttcct ataaaatatc attagaagaa aaaatataat ctaaattata
    27421 cttttgggga agggggttta aaatggatat aaaacagttg aaatattttg ttgaaatagt
    27481 gaaatctggt tttaatttat caatagcatc taagacactt cacatatctc aaccagcatt
    27541 aagtcaaatt ataaaaaatt ttgaagaaag tgaagatgta tatttatttg aaaggtataa
    27601 aggcaggtta aatgggttaa caccagcagg agaaagattt tatgtaaatg cagaacatat
    27661 cataaatgaa tacaaaaata tgatggaaga tttaagagaa gattcagttc aatttaaagg
    27721 aaaagttaga ataggaatac ctccacttat acttggaata gcattttcag atgtagttgc
    27781 acagttagtt gcaaataatc cagatataga atttgatata gtagagaagg gtgcatatga
    27841 cttaaataga atgcttatct tacaggaatt agattatgca gtattgttac atccacataa
    27901 aattgataaa agtgtagtaa ctgaacatgt gcttcaagaa gatgaactta cagcattttt
    27961 aaatataaat catccacttg ctaagaagga taagatagat tggaacgatt tgaatgagca
    28021 gttacttgca atatttaatc ctacttttat gatacatcat aagctaatgc aaaaatttac
    28081 tgatgaaaat gttaagttaa aaagatatat aatgtctggt tcatgggatt ttttgttgct
    28141 atcaaccaca aattctgatt ttataacaat tctaccatca ccagttcatg atttttttga
    28201 aaatagcaaa attatagaaa agccttttaa tcatcctatt acatggaaag ttatattatg
    28261 tagacctaaa aaagaacgtt atagccatgt agaccaacat gtatttaaat ttattataga
    28321 tttctttagt aaaaagaact cttaaaaatg ttattaaaat aacaaataaa taaatcaatt
    28381 tttattataa taaactatta gtttttcatc tatatgtttg agagcaataa taggttttac
    28441 ataacatggt agagtagtat ttaaagttat aatatacaaa atatgtattt ttttaaacga
    28501 aaaataaaaa cagtgtatat ctttagataa tcactgtttt tattttctat ataaaatttg
    28561 ttaagtatta ctagaaaatt ttttgcttat tatagtcaaa ctcatgactg ttaaaaatat
    28621 aagtaatcct ggaaatacag ctaaatataa cttgtcaaaa atatgtcttt gagcatcttg
    28681 aagcatatta ccccatgatg caagaggcaa ttggattcca agacctaaaa aactaagagc
    28741 agattcagtt aagatagcat tagcaatatt taacgaacca gcaactacaa tagtctcttt
    28801 taagtttaaa actatatgtc ttaatagtat atttctagtc ttgataccag tagctttagc
    28861 agctagacag tattcatttt cctttatttt taatgtttcg ccacgtacaa ttcttgaaac
    28921 ttccatccaa cttaagaatc caataattag tataatagtg gaaactttag gtgttagata
    28981 agtatttaat acaatcaaaa ctaagaaact tggtatactc ataagcatat ctattaatct
    29041 catcaagaat tggtctagct tacctccaat atatccacta ataacaccta cagcagtacc
    29101 aaatactaca gaaataagca ttgataaaat accaacacta agtgaaatcc ttccaccata
    29161 aagagctcta gagaagtaat ctctaccaag ttcgtctgta ccaaagatat gctttaagct
    29221 aggttcttga agtttagaag tagtatcaat tgcattagag tctatattta atataaaaga
    29281 agctataatt aatatgatta ttatgctaag agtaatacat gcaaaagcaa aggatttatc
    29341 tttttttaat ttttttaaga tacttgtaaa cacaactaga atcctccttt gctagatttt
    29401 attttagggt caattaataa gtatagtata tcagcaacta aattacctaa aattaaaaat
    29461 agtgctgata acatagttat tgccataatt acagtataat ctcttgaaaa aattgaatca
    29521 atcattaatc ttcccatacc tggccaagag aaaactactt ctgtaacaaa tgcaccagtg
    29581 actaattttt gtatactcat gcctaggatt gtaaccaatg atgtcagtga atttttcaag
    29641 acatgtttaa atagtatgac tttttctgat aatccttttg ctcttgctgt aataacatat
    29701 tgttttttta attgttcatt tacactagct tctacataat ttgtaaaaat agcagtatta
    29761 tatagaccta aagttattac aggcaaaact aaatgtttta ctaagtctcc aatactatca
    29821 tttccaatag tatgcatacc tcctgatggg aaaatgttaa gtttaactga aaaaattgaa
    29881 atgagtatca tacctatcca aaaagaaggt atagaaacac ctatataatt aaaaacagat
    29941 gatactttac tagttattga atttttattt acagctaaaa atattccaag aggtatagct
    30001 accaatattg aaaatatcaa agaagtaccc ataagaataa atgtatttcc cattttttcc
    30061 ataatttgtt ctgtaacggg catatggttt atataagaaa tacctaagtt tccacttaaa
    30121 gtgtttttca accagtttaa atatcttata tatactggtt tatcaagccc catattaacc
    30181 cttatatttt caatatcctg tgaagacatt aaaggattta tatatgcatc tgctgggtca
    30241 ccaggagcta attctaaaag tccaaatgat aatatagata ttaagattaa cataggtatt
    30301 aaaactttta ctctattcaa tattaatttt aacataagta aattctccta attaaaagag
    30361 atgtctcgtt ttaaaataat aaaacaatat tatttttaga catctccttt tctaattaat
    30421 aatataaaat tttacatcaa ttattctaca tacagtttag accaatcttc aaataatatt
    30481 gcaggagatg gtttagcatc ttttattcct tttagatttt tttgagctgc cattaaattt
    30541 tgttcataat ctatagtgta aataggcgca tccttagata tatcctgttg tatttgctta
    30601 tatatttctt cacgtttttc cttgtttaac tcagtaaatc ctttttccca taaagcatct
    30661 atttcagcat tagaatattt agtttggttg caatattgac cagtttcaaa catacctcta
    30721 tatgggtttg gttctaatcc ccaagcataa ccatttacat acatatcaca ttcagtgcta
    30781 ttactaaata gtatattgaa gaatgcctta ctttcataag gaactatttc agcttctata
    30841 ccaatttttt tcaactcttg ttgagctact aaggcatagt ttttatgccc aaatctacct
    30901 gtgttataac ctatttttaa tttatcaatt ttaacaccac ttttatccaa taagtcttta
    30961 gccttatttg tatcttggtc atatccttca acaccttctt ctgtatagaa gtctgcttct
    31021 ggaactaata tagatttagc aggtacagaa ccttcttttc catatgctga ttctatcatt
    31081 tcatttctat ttaaggcata tgataaggct tgtcttactt ctttgttagc catgtttgat
    31141 atattttggt taaatacaat gtagtttaat ctttcttcac tataagtata agtttgtaag
    31201 tttgagtttg attttgcctt ttcatatcct tcagcagatg tcttcataag tgaaatttcg
    31261 ccattattta aagctgcttc ttgagatgct tcattaggaa ttatttttag cgctatagaa
    31321 tctgccttag gttctccacc aaaataatct gcattctttt caaatactat gctttcacct
    31381 tttttccatt ccttaaactt gaatgcacca gaaccaactg gattattatt tttttctgac
    31441 ttagctatat ttgattctcc ttcaaacaca tgttttggta ttggagaaat tttagatata
    31501 ccatataaga aagattcact tgcagttggt aaagtaatct taactgttaa atcatcaatt
    31561 ttttctactt taactggttt ctcacccacc aataagtatt gtcttgatgg tgaattttgt
    31621 ttttcgtcta aaactgtatt aaatgtaaat aagacatctt cagcagttat tggctttcca
    31681 tcatgccatt ttaaattgga ttttaatttt atagttaatt ccttactatc tttaaaatct
    31741 actttttctg ctaaataatt tactaaattt cctttttcat aagtataaag aggagcataa
    31801 actatatttg atgcaagaga gtcttcttta acgtttgcaa aagctccatt gattatatct
    31861 gggtcacttg ccataggtat tacaacagtt ccaccagatt tgcttccact agacttgtct
    31921 ttatcattgg aagcacaggc tgtaaatgta agcattaatg atgaaactaa gattaatgat
    31981 gccaattttt taaatttcat attcttataa aacctccata aaataatatt taacacatat
    32041 tttttattgt ataatagtaa tcattattaa atcaatatat atagaaatta actattatat
    32101 tattataatt aatagaaaaa aataataaaa tgtaagaaaa tgttgtaatt taaattttta
    32161 taaaatacaa tcaaaaggtg tatgttatct taagtaattt tgctgagagg agaaaataat
    32221 gagtaaagca ctaattgagg ttaaagattt aaaagtttat tttcatacag ataaagggat
    32281 tgtaaagagt gtaaatgaag taagttttaa tataaatgaa ggtgaaacta ttggaatagt
    32341 tggagaatca ggatgtggta agtctgtaac agccatgtct ttgatgaagt tacttccaac
    32401 atcaaaaata gaaggtggag aaataatctt tcgaggcaaa gacattttaa agatgaatga
    32461 agatgaactt atgggaataa gagggaatga gatatcaatg atttttcaag aaccaatgac
    32521 atctttaaac ccagcgttta ctgttggaag tcaaatcatt gaaggtatta tgatacatca
    32581 agatttatca aaggaagaag ctaagaaaaa ggtaatcgat atgataaaac tggtagaaat
    32641 accaagagct gaagaaatat ataattcata tccacatgaa ttatcaggtg gtatgagaca
    32701 acgaataatg atagctatgg ctttatcatg taatccaaaa ttacttatag ctgatgaacc
    32761 aacaacagct ttagatgtta ctattcaagc tcagatttta gatataatga aaaatataaa
    32821 agaaaaatta aacacatcta taatgatgat tacacatgat ttgggagtag ttgcagaaat
    32881 gtgtgacaaa gtattagtga tgtatgctgg caaaattata gaagtggcag aggttgttga
    32941 attatttaaa aatccaaaac atccttatac tattggactt ctaaaatcaa agcctgctct
    33001 tggaaaaaac aaagataaaa gattgtattc gattccagga caagtaccaa atccaattgg
    33061 aatgccagat tcatgttatt ttagtgatag atgtgaaaaa gtatgtgata aatgtagaac
    33121 tcagatacct cctttaatag aattgaatag tggtcatagt attgcatgtt ggttatataa
    33181 aaaggaggag atataattga aaaaagaaga gagtttatta aaagtagaag gtcttaaaaa
    33241 gtactttgaa gttaaatctg gattatttga taagaaaatt aaatctgtaa aggcagtaga
    33301 tggaatttct tttgatttaa aaaaaggaga aactttggct atagttggag aatcgggatg
    33361 tggaaaatca actgcaggaa gaaccattct tagattaatt gaaaaaactg aaggtaaagt
    33421 tgaatttaat ggtgtagata tatataagtt agagaaggaa gaacttagaa agttaagacc
    33481 caaaatgcaa atgatatttc aagacccata tagttcatta aatccaagat taaatgtgga
    33541 acaaataata tccgaggcag ttatagaaca taatttagtg ccaaagaatg aaataaaaga
    33601 taatatatta aatgttgtag aaagttgtgg tctttctaag taccatttaa atagaaaacc
    33661 aggtgagttt tctggaggtc aaagacagag aataggtata gcaagagcat tagcattaaa
    33721 tccagaattt atagtttgtg atgaacctgt ttctgcacta gatgtttcta ttcaatctca
    33781 aattataaat ttacttatgg atttacagga taaatataaa ctttcatatt tatttatatc
    33841 tcatgatttg agtgtagtaa atcatattgc acatagagtt tgtgttatgt atctaggttc
    33901 tataatagaa attgcaacta aaggagaact ttacttacat tctcaacatc catatacaaa
    33961 agcacttatg agcgcagtac caatacaaga ccctacaatt aagaaaaata gaattatatt
    34021 aaaaggagac ataccatctc caacaaatcc accaagtgga tgtaagtttc atacaagatg
    34081 cccttattgt aaggaaatat gcaaaaaaga gattccagtt ttaaaagaaa tatcaagaaa
    34141 tcattttgtt gcatgtcatc tagtatagat atttcaaaaa ttaaatttga tgtatataat
    34201 aagaataata gaataaactg aaaatttttt tgattaagat gggatttcat gctgttaata
    34261 ttaagttagt tgatatataa gtttaatcta taatattatt attgaaaaga gaggtagtta
    34321 tgtcaatttt agtaacattg ttgggattat tggtttgtac aacgatttca actgtattta
    34381 gcaaaaagtg gagtaatatt cctcttgcaa tatatcaaat tgtactaggg attatattgt
    34441 ctattttgcc atttaaattc tcattttcat ttaatccaga aatatttgta atatgtatca
    34501 tagctccatt actattcagt gaaggacaaa atgtatcgag aaaagagcta ttagagctta
    34561 gaaaaccaat tttgcttctt gcatttggtt tagttttaat aaccgtgttt gcaggtggaa
    34621 tctttataca ttttcttatt cctgggatgc cattatctgt ttcatttgct ttggcagcag
    34681 taatatctcc aactgattta gtagcagtaa aatcaattac acaaggatta aattttccta
    34741 aaaatataat gagtatactt gaaggtgaat ctttattaaa tgatgctgct ggagtagtag
    34801 catttaaggt tgcagtactt gctacagtta caggagtttt ttcaatagaa gaggcaggga
    34861 ttcaatttat gattacagct tttggaggaa taatagtagg aagtatttta ggatacataa
    34921 ttataaaaat aagacttagt ttacacaagt ggaatttgga agaaattcca atggtaatag
    34981 ttatacaaat aatgactcca ctttttgtat attttgtagc agaagaaata ggagtatctg
    35041 gaatcctagc tgttgtaatg gcagggatag ctcatggaat tgaaaaagag catttacaaa
    35101 atacaactac taaattaagg ataatatcag acaatacttg gtatgttttg gagtatgtat
    35161 tgaatggatt tgtctttaca ctgcttggtt ttttgttacc aagtatatat acaggactta
    35221 gtagtaaaaa tgaaaatatg gcattggaat tgacttttat ttctacttta attgtactta
    35281 ttttatttgt tataagattt atatgggtat atttatggca taattctttt ataaaaccta
    35341 aaaagaatcc tttaaataat ttttttgtag gttttttagg ttttaaggat gaagaagtta
    35401 taaaagagag tatttctaaa tgtaaatact cactaatagt tgctacttgt ggtgttcatg
    35461 gtacatttac actagcaact gccttatcta taccttttta tttggctgat aaagatgtat
    35521 ttccaatgag agatactgtt ttatttatat catcagaagt tattcttata agtttagtat
    35581 tggcaacaat tttactgcca agattattaa aaaataacgt acaagagtca gaacctttat
    35641 tatctgataa agaggcatat aaactaattt tgaaagaagc cattttaaaa cttagtaatg
    35701 aaaaagcaca agaagtacaa ccagttatac atgatttaaa tgaacagttg gtagactcag
    35761 aaaaaggaat actaaatact ccagataata aacttataag tatgatgatt gcagaagcag
    35821 accaacaggg attattagct gttttgaaac tattagaaga tggtaaaatt tctgatagag
    35881 cctttaaatt gtatagattt tatattacaa aatcaagaaa atctgttcaa aaatctatat
    35941 ttaaaattgt aaaattaaaa ataagcatgt ggattataaa tagaaaagta aagaaagaag
    36001 taaaagaaaa aattacaaaa atagtatctg agaataaaga aataatcaaa gagtttcacc
    36061 atgcatatgc actttctact aaaactgcta tatcatttat aaataaaaat actgatgaag
    36121 ataacagaca tgaggcctta atagcaatca gattctataa tcgttatttg aattggatta
    36181 ctagaaagat tcaaaatgat gataagtttg aggcaagagt agaatactac agagtaatgg
    36241 cgatacaaag tcaaagagac agtgttcaac aattagttga aactaagcaa atatcaagag
    36301 aagtggctaa agagttattg gaaaatattc tctatgatga aatgatgatg atagaatcat
    36361 aaatataatg gaataaaatt tgatttttga tgttaaaatt aatactaaaa aataggaaat
    36421 agttttcaat aatttacttg acatatatat ttatttataa tagaataaaa ccataaattg
    36481 aatacaatat ataagcatta acaagctgga ttgttactgg taagcaggca atacctaggt
    36541 taaggtatac aggtgaattg tatcttaatt taggtatttt tttatgaata agggaaattg
    36601 tattttggga gagatattga ttatgattat tcaacaaatt tataataata atgttgtatt
    36661 agttttagat gaaaatataa aaaaagaatt aatattaaca ggatgtggaa ttgggtttca
    36721 aaaaaagaaa ggtcaagaaa tagataaaag taaaattgaa agaacttttg taatacaaga
    36781 tgaaagcttt ttagataaaa ttagtaaact agcaagccag gttgatgaga aattttttga
    36841 ggttagtact gaaataattg cttatgcaga ggagaacttg aatactaagc tttatgaata
    36901 tatttatgta gcacttactg accatattgc atttgcaatt aaaagatatc atgaaaacat
    36961 aactataaag aatgatttac tacatgagat aaaaagaata cataaaaaag aatatgagat
    37021 tgggaaatgg gctgtagatt atataaataa agaatttgat gtgaaatttc cagtagatga
    37081 agctggtttt atagcaatgc atatagtaaa ttcaaactat aagggaagtt caaaggaatc
    37141 actattaatt actaaaattg taaaagacat actaaatatt ataagatatt attatagagt
    37201 tgaatttaaa gaagatgata taaattatga taggcttctg actcatttaa aattctttgc
    37261 aaaacgatta gtaaaaaaag aaaaaatcaa tgatacaaat aatgaaatta tagatataat
    37321 taaagtaaag tatgaaaagg attatgattg tgcatataaa ataaaaactc atgtagaaaa
    37381 gaattatgat tactatgtaa gtcaagatga actactttat ttaacattac atatcaaaag
    37441 agttatatct gtactgaatt tataataaag agtagtttaa t
//
LOCUS       DAMJWU010000030        36662 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMJWU010000030 DAMJWU010000000
VERSION     DAMJWU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.030"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            176..553
                     /locus_tag="PC460_001653"
     CDS             176..553
                     /locus_tag="PC460_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2600540.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            546..1694
                     /locus_tag="PC460_001654"
     CDS             546..1694
                     /locus_tag="PC460_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2600541.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1711..2313
                     /locus_tag="PC460_001655"
     CDS             1711..2313
                     /locus_tag="PC460_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2600542.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2406..3428)
                     /locus_tag="PC460_001656"
     CDS             complement(2406..3428)
                     /locus_tag="PC460_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2600543.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3757..3939)
                     /locus_tag="PC460_001657"
     CDS             complement(3757..3939)
                     /locus_tag="PC460_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600544.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4252..4671
                     /locus_tag="PC460_001658"
     CDS             4252..4671
                     /locus_tag="PC460_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF2600545.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            4802..6643
                     /locus_tag="PC460_001659"
     CDS             4802..6643
                     /locus_tag="PC460_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2600546.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6670..8556
                     /locus_tag="PC460_001660"
     CDS             6670..8556
                     /locus_tag="PC460_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2600547.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNETRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            8564..10564
                     /locus_tag="PC460_001661"
     CDS             8564..10564
                     /locus_tag="PC460_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2600548.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            10826..12085
                     /locus_tag="PC460_001662"
     CDS             10826..12085
                     /locus_tag="PC460_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2600549.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            12146..13933
                     /gene="iorA"
                     /locus_tag="PC460_001663"
     CDS             12146..13933
                     /gene="iorA"
                     /locus_tag="PC460_001663"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF2600550.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            13935..14510
                     /locus_tag="PC460_001664"
     CDS             13935..14510
                     /locus_tag="PC460_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF2600551.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            14563..15633
                     /gene="buk"
                     /locus_tag="PC460_001665"
     CDS             14563..15633
                     /gene="buk"
                     /locus_tag="PC460_001665"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2600552.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            15851..16552
                     /locus_tag="PC460_001666"
     CDS             15851..16552
                     /locus_tag="PC460_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600553.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            16612..17118
                     /locus_tag="PC460_001667"
     CDS             16612..17118
                     /locus_tag="PC460_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2600554.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            17293..18315
                     /locus_tag="PC460_001668"
     CDS             17293..18315
                     /locus_tag="PC460_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2600555.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(18462..18770)
                     /locus_tag="PC460_001669"
     CDS             complement(18462..18770)
                     /locus_tag="PC460_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2600556.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(18830..19258)
                     /locus_tag="PC460_001670"
     CDS             complement(18830..19258)
                     /locus_tag="PC460_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600557.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(19481..20938)
                     /locus_tag="PC460_001671"
     CDS             complement(19481..20938)
                     /locus_tag="PC460_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2600558.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            21356..22270
                     /gene="nadA"
                     /locus_tag="PC460_001672"
     CDS             21356..22270
                     /gene="nadA"
                     /locus_tag="PC460_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF2600559.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            22283..23584
                     /locus_tag="PC460_001673"
     CDS             22283..23584
                     /locus_tag="PC460_001673"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF2600560.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            23565..24401
                     /gene="nadC"
                     /locus_tag="PC460_001674"
     CDS             23565..24401
                     /gene="nadC"
                     /locus_tag="PC460_001674"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF2600561.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            24657..24989
                     /locus_tag="PC460_001675"
     CDS             24657..24989
                     /locus_tag="PC460_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF2600562.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            25151..25336
                     /locus_tag="PC460_001676"
     CDS             25151..25336
                     /locus_tag="PC460_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600563.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            25363..26448
                     /locus_tag="PC460_001677"
     CDS             25363..26448
                     /locus_tag="PC460_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2600564.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            26441..26899
                     /locus_tag="PC460_001678"
     CDS             26441..26899
                     /locus_tag="PC460_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600565.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            26934..28025
                     /locus_tag="PC460_001679"
     CDS             26934..28025
                     /locus_tag="PC460_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2600566.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            28030..28254
                     /locus_tag="PC460_001680"
     CDS             28030..28254
                     /locus_tag="PC460_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600567.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            28264..28827
                     /locus_tag="PC460_001681"
     CDS             28264..28827
                     /locus_tag="PC460_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600568.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            28788..29417
                     /locus_tag="PC460_001682"
     CDS             28788..29417
                     /locus_tag="PC460_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600569.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            29594..30346
                     /locus_tag="PC460_001683"
     CDS             29594..30346
                     /locus_tag="PC460_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600570.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            30359..30844
                     /locus_tag="PC460_001684"
     CDS             30359..30844
                     /locus_tag="PC460_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600571.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            30851..31000
                     /locus_tag="PC460_001685"
     CDS             30851..31000
                     /locus_tag="PC460_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600572.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(31137..31958)
                     /gene="yidA"
                     /locus_tag="PC460_001686"
     CDS             complement(31137..31958)
                     /gene="yidA"
                     /locus_tag="PC460_001686"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF2600573.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            32451..34145
                     /locus_tag="PC460_001687"
     CDS             32451..34145
                     /locus_tag="PC460_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF2600574.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      34477..34587
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            34691..35062
                     /locus_tag="PC460_001688"
     CDS             34691..35062
                     /locus_tag="PC460_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF2600575.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            35159..36106
                     /gene="trxB"
                     /locus_tag="PC460_001689"
     CDS             35159..36106
                     /gene="trxB"
                     /locus_tag="PC460_001689"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2600576.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            36210..36527
                     /locus_tag="PC460_001690"
     CDS             36210..36527
                     /locus_tag="PC460_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF2600577.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT        14506 a         3865 c         6339 g        11952 t
ORIGIN      
        1 tatgtaaaaa gtaaaatatt taaatagata tattttaaaa tctaactttt aatattgtaa
       61 aaaaattaga aatttaacaa aaagtacaaa aattcacatt tttttcataa agtgtttgac
      121 taaaaaattc acatgttgta gaattaaggt agcaggagtt ttgagggagg tgtaaataat
      181 gataagaaaa ataccacaag cagaactaaa agtaatgaaa ttcatttgga aagtagatgt
      241 tacagtgaca tcaaaagatg ttattgaagc tatggaacaa aaatatggct ggaaacaaac
      301 caccacactg acacttttat ctaggctggt aaaaagagga ttcttagatg ctcaaaaaat
      361 agacagatat acacattaca caataagagt aaaacacaaa gattatttaa gttttgagac
      421 aaaggatttc ctaagtaata tacatgatga ctctttaaga agcttaattt cagcattaca
      481 tgatgatgaa aatataggta aagataagct agattttcta gaaagttatt ttgataattt
      541 aaaggatgaa taattatatg ctatatgttt ttgaaaatct tataaagaca actattgtat
      601 gtagtcttgg tatttgtcta ttattatttt tgaaaagata tttatttaag aagtttagta
      661 agagattcaa ttattatatt tggttgataa tagtctttag aatgttactt tttctattta
      721 attatacaat agtgtatgaa gtaaaagaac ccaaagaaaa tgcattagga aataatatta
      781 cacaaattga tatttctaca gataataatc ttatgctgta tgtagcttat ttatggttat
      841 ttgtaactat agtcattgca gtttatacat ttataaagta tacaagattt aaaaatctag
      901 ttgttgatgt atcttatgat attgaagata atgatataaa ctgtatctac aaaaacttgt
      961 taaaagaatt aaatataaaa aagaaaattg aattaagagg ttctgatgaa ttgataagtc
     1021 cagcaggtat gggattattt aaatcttaca tattcttacc agactatcct tatagtaaag
     1081 atgaactaac ttggattttg aaacatgaat taatgcattt taaaaataaa gatattttaa
     1141 taaagttttt agttttgtct gttagaataa tatattggtt taatccactt gtatatgtaa
     1201 tgagcaataa agtaaatcta gattgtgaat tatgttgtga tgaaagtgtt ttggtagaat
     1261 gttctttaaa agataaaaaa gaatatgcac tagcacttat aaagtcaatt aagtttagta
     1321 aaaattataa tagtaagatt ttgacaaccg agtttgataa gacaaatttg gaaaagagac
     1381 ttgagagtat tgttaaaaag aaaggcaaaa gtggaatact aattgctgtt ttagtattta
     1441 tgacttctat aacatctttt gttgaagttg atgctcaagt tagaattgat gagggtaata
     1501 atggaatgaa taacaatatt aatgaatttt tattgaataa gacgactgat aaaattataa
     1561 atatttatta taaagatgca ccaaatgaaa ttaggaaatt ttatgaagat aaatgcgaat
     1621 tagaaggtaa aatacctaga gactatgaca taatagaaat ggatatgaaa gattataatc
     1681 aattaataaa ataattataa ggaggatttt atgttaacaa aattaagaaa aatagtagct
     1741 attatgtgtt gcttattgct tacttttaca agtgtggcat ttgctgacac aagtaaagag
     1801 aaaggaggaa gtaaagagac tgttccagag aaaaacctag attcttttac tctttctggg
     1861 tatatatata catatgaaaa tgcgccacaa gaaataagag atgaacataa acaaaattgt
     1921 gaagagataa atatagaccc taagccagat gatgagatat ttgttccaga atcagctaat
     1981 ctaatgaatg aggacaatag taaaggtgtt tatgcaagtt atactgtttc ttataatata
     2041 ggtgctaaaa ctataacaat tatgagtaat gagtatttga ctataagtac aacaaaagtt
     2101 atacgaaagg gtaatagcgg aaaagaagta aaagctgcac aaatcatgtt aactttactt
     2161 ggttataatg taggtataga tagttctttt ggaagtaaga catataatgc agttgtaagt
     2221 ttccaaaaaa aatacggttt gtctgcagat ggaataattg gtccagctac ttgggataaa
     2281 ttgggtagac taactgaccc tactctttca taggattaaa atctaaaaat atattaaata
     2341 gtgtctcaat atgatttttc aatttaaatg agacactatt taaatataaa tgttataaaa
     2401 ctattttaca ataaattcca tataactcta ccatctatat ccttaacatt ttgacaacca
     2461 gtcaaaatca tagtctcaca taactcattc ctaaccttct ctataaaaaa ctccacacca
     2521 tcaagaccac cgccaaaaga agcagtaaca aaaggtcttc ccatcaaaac agcatcagca
     2581 cccaatccaa gcatcttaac tacatcaaca ccagtcctaa ctccaccatc aacaagtatt
     2641 gttatctttc cctttacaga cttagctata tctggaagta cctcacaagt accaggagta
     2701 taatcaagta ctcttccacc atgatttgaa acaataatgg cactagcacc agactctaca
     2761 gccatcaaag catcatctac agtcataatt ccttttataa taaaaggtaa tctagtggat
     2821 tcaaccaatt ctctaatctc atcaattgtt ttaggagaaa aaggattttc ttgaaattga
     2881 ttattaataa gtccacaagc atcaaggtct acaccaactg caaaagcacc tgcttcttca
     2941 gacaatctaa ttttatctat tatctttgaa ttattccaag gctttataaa tacaattcca
     3001 tttcccctat tatccttcaa tacatctaaa ttatccagta aaaaagtatc tacattagta
     3061 tcgccaacca tagcataaat tccagaatta gaacaacctc taacaacagg ctctatgtat
     3121 tctttttcgg aaactttacc acccatgttc aatatagtac ctgatacagg tgcagcgaat
     3181 ataggagaac tcatttttct accaaataat tctatggatg tatctggctt tgaaacatta
     3241 tgtatgactc tcatatttat ctttattttt tctagacttt ttctattttc aatgaaagac
     3301 gaaccacttc ctttaccacc cataccagga acatctccag cacaagctaa tccattacaa
     3361 attttacata ctttacatgt tccattaaag ttctctctag ctgattttag taagtcattg
     3421 taatccatac aaaatctctc ctttttataa tattaaaatt gagtattact atattgaaaa
     3481 tatcttaatt gtaaatatag ggatgttatt atgatgagct aaactagata atacatatta
     3541 gatatttttt attgttgtag atagttaaat tatatcatat tagatagtca aattttgaaa
     3601 aaaactaact tatttaagta tacattttga aaaaattaaa aaagctatat atagattttt
     3661 aaagaaatct atatatagct tttaagttta tgtcaatctt ttttgttaat gaataaataa
     3721 tatagaatac aatatacaaa aaagtttctt gaaatgctaa gataatcccc atttacaaag
     3781 caacatcatt atactgtgta aatttgaatc aacaatgttt tcaccatatt tcatcataaa
     3841 atcctctaat tgtgtgtcaa taaatgaata atatccactt atactatcga tgtatgcagt
     3901 cattaagtgt atacattcat cacttttatc tttatacata tatatctccc cctatataca
     3961 atatataaat ttagatacca aatcgtatca tattaaatag ttaagtgtgt aacatctatg
     4021 cagttatata tttatatcat tatattaata actaaagtga gttttgacaa tagattttgg
     4081 gaaactggat gttcaaagtg caaacttgga ttttaaaatc attaattaag atagcgcata
     4141 ggaatatatg tatacataac ttaattataa agattataaa caattacttt tattaacttt
     4201 atttaaatgg tagaataact tggagattaa taattataag gaggatataa tatgagttta
     4261 tataagttta tatatgatga taaggaatat ttattaaaag aggagaactg tagtgcttta
     4321 ataaatgatg aagaaaatcc agttcaagga gtaagtattt caaaaataat tgatatactt
     4381 aatgaagcag aagaagttga ttttgatgta gaatattatc aagaagcatg tccattatgt
     4441 ttagaaggag taaaagaaaa gaaaaaattc tttccttttt tagaatatca cttttatatt
     4501 ttcagtaaag atggagaata tgtaataagc aatatatctg ttgattataa aggactatct
     4561 tttaataaac tatcaagggc aaataaagtt gataatagtt atattgtaag tgtgataatt
     4621 tgcgaaaatt gtcaggatta tatagttcag atagaaaatt gtatagttta gtgtaaagta
     4681 tgtcttaatt taagagaaat ttctaaattg agacatcaac ttttttcaaa aatagtatgg
     4741 aaaattacta aatttatgat ataatgtaag tcggaggtga gttacaatat atagtaaata
     4801 tttggaagag atagatgata caattttaga ttcaatgttt aaactaacct tgaatgaaag
     4861 attattgact aataacgatt ttattgaaaa taaaattgat gtagtaaaga atttaattga
     4921 ttgctataaa actgaacaaa atagtttaat gttattaaaa tcaaattttt tgctagcttt
     4981 attatatggt attcaagggt ttagtgaaaa aatgaaagag tatattttga tttcctgtaa
     5041 atatattgat aaatgcttac ccaaagatta taaattttta gcacgatttt attcacaaat
     5101 agctatttta agtttaaaaa atgaagatgt aaataaggca aatttatata tggataaatt
     5161 taatcttatt tgtgatgaaa ataattttct aattgaagaa attattttta aatcacaact
     5221 tatttacatt aaggcaagta agtgtataga atcttctgta ataattaaag agatagaaac
     5281 gttgtatata aagataaaag aaattaacga ttttaattgt aaaaaaatat atttttttat
     5341 tattggtaaa atatattttt tgtttttgga tgatgctcta attgctaagg taaatttttt
     5401 aaaagcaaga aaatatgcag atttactaaa ggatatgcaa gtatcttcac tatgtaatat
     5461 aaagataggt gagtgtgaat gttcgtttga aaattatgaa tgttccatgg aatatttcaa
     5521 tgaagtaata agcaataaaa agtatagaaa tgtaaatata attcaaaaat atagagcatc
     5581 aaacaacata actaaaatat taataaagac tagaaattat cctgtcgcaa taaataattt
     5641 agcaaaatct gaagtatatc tagaaaaaat taaaaatcat agcttaaaag aagtagagaa
     5701 gtttgattta tttataagtt tagccatgta ttatgcaaaa agtaatgaaa aatcttttga
     5761 acaatcaata tgctatttag atagagctaa aaacatatta aatatgataa aaaatataga
     5821 agaatatata atatatgatt tagaaataat ttatcaccag gcaaagatat attatatctt
     5881 tggatattat gataatgcct taaacacaaa taaaaaactc cttgaaaaat ctaaaaatgc
     5941 taaaaattat aattatgtga agttagcata caagagtata tatttgtgtt ttgaaaagat
     6001 tcaaaattat gaaatgagta tgaaatactt taaaatgtat tatgagttaa aaatgatgta
     6061 tataaaagct cgaaatagaa attatataga ttccttaaat tatagacatg agtatataga
     6121 aaaagaaata aataacatga gaaaagtaaa acttgattta gataaaaaaa agtatgttga
     6181 ccatttgaca agtgcatata atagaacttt tttgaacgat tttttagaaa aacaagaagt
     6241 tagtgaatat tgcacagcat ttatgataga tgtagattac tttaaagaat acaatgatac
     6301 ttatggacac tataatggag atattgctct taaaaatata actataataa taaaaaaata
     6361 tctaaaaaaa gacatgctta tgataaggta tggtggagaa gaatttctga tattatctgt
     6421 atgtaaagat tataaaaaaa gtaaaatatt tggaagaaaa ttatgtagga ttgtaaagaa
     6481 atttttaaac aataatttga cagttagtat tggcattgat acatgtaaaa atagtagtat
     6541 agatataaaa gaaataattg aaaatgctga taaagcttta tataaagcaa aggaaagtgg
     6601 tagaaataga tgtttgcatt atcatgattt taagaatttt taactgttga aataggattg
     6661 gtgatttact tgaaaaggag tttaatagaa gatttaaaga gaaatataaa caagatagaa
     6721 tacgatatgt tgactaaatt ctatctaaat ttgacagatt taaactacat cttggattat
     6781 cacaaagacc ttttaaatac aaaattagaa gataatagta gagtctatac tttatattta
     6841 attagtctta taaactatat aaaaaaagat agagaagcat catacaacta tttgatagag
     6901 tcattaaagt atattaaaaa attgtcatta aaagatgatt cagatataat atctaaaatt
     6961 tatatttatc ttactttatc agctatttct ataagaaaat atgataaggt aaataatttt
     7021 tatttctcag caaaaaaaat tataagacaa aatcatttaa atgagttgtt ggtattgtta
     7081 aatatgagtt tatgtgtaga aataagtgct ttatatcaga ccaaaacgga tgtaatagct
     7141 ttagtagaag aagtgtcttt ttttaaaact ttaaaaaata aagctcttgt aagtagggca
     7201 aattatatat ttgggagagt ttatctatat ttatttaata actttataaa aagtatggaa
     7261 tacttaattg aatgtctaaa attggctcaa gaaaataatt tatactctat agaggctttt
     7321 tgtaaatgta taataagttt gtgttattta gagagtggaa ataatgtaga agctataaaa
     7381 tacataaata atgtattaga tgttgcacac agtaataact caatatcagt aactgaaaga
     7441 gtgtctataa aaatagacct tatatgggca tatttaaaag aagatatgaa taaagaagca
     7501 gaagttattt tattaaagac tataaaatta atgaaagttg ttgaaggtgt atataaggat
     7561 tatttatatt catttatatt tttatattca gctgaaattg agttaaagaa agataattgt
     7621 agttttgaaa aggtaaacac ctatttaatt ttatcaaaaa atatatataa aagttttgga
     7681 gatgttttta tatataattc taaactagat tacatatata agttgtatgg agatttatat
     7741 ataaaatttg gtaatataga tgaaggtata agttgttata taaaaggttt aaatttagtt
     7801 aaaaagagtt cattgaatct tagaaaaata tctatatttt atgggctaat agcaaatgga
     7861 tatgagctaa aaaaagattt taaactggca atagaatatt ataaaagtat ggattggtat
     7921 tctgataggt gggaaaaaaa taattcctat aaaacatctc aagcaattca caaacaatat
     7981 gaactcagac aaaaacaaga gagtttaaga ttactagtgg atgataataa gaaattagaa
     8041 aatgatattt taaaagatgg acttactaaa ctatataata gaagatatct ggaacagcag
     8101 ttggatttat ttaatgaaac cagagacaaa aatatggtcg caatactggt cgatatagat
     8161 tgttttaaaa gttataatga taattatggt catctagcag gagataaagt tatagttaag
     8221 gtaactgata ttataagaag tgtatttata gatattaccg aacacataat cagatatggg
     8281 ggagaagaaa ttttaatatt agtagattct atagaatcaa tcggtgttga agagattgat
     8341 aatatagaat tatatattaa aagagttttc aaacttcttg aatttgaggg tattgaacat
     8401 atatattcta aaataaaaaa ctatataact ataagtgctg gagtaagtat aaaaagatgt
     8461 cgtagtagaa aagatgttga aatattaatt gaagatgcag ataagaacct atataaatca
     8521 aaaaaagcag gtagaaatca atacattata atttaaaatt gaggtgattt tgttgcgaaa
     8581 aaaaatagga attatagcct ctgatattga attaaaagaa agaatagaag aattatatag
     8641 agaagatgta gaaaatggaa ctataataat cgacatactt aacctagact taatggaaaa
     8701 tcaaggtcgt atacttgttg aaaaaggtgc ccaagctata attggtagag gtggaggtta
     8761 tagtttagta atagatacag taaatgtacc tgttatacca atgaatatga agtcaactga
     8821 ccttttaaga gcaatagaaa tagcaaagaa atattctaaa aaagtagtat tgattcttgg
     8881 agacaatgaa gtttcttttg actatgttgg ctggagaaat gtcataagta ctgaaataac
     8941 tgaagaatgg tttgaatcta agtatgagat aagaagtaaa gttgtaaaat atatagacca
     9001 aaaagatgaa attgtaatcg taggaggagg tttggcatgt tcttttgcaa ggcaatatgg
     9061 aattgatagt gtatttgcaa ctgctagtga tgaatctata agagaagctg tagaatattg
     9121 taaaaaatta ttagatacat taggtgaaga aaagtttaat aatgaagtgt tacgaaatat
     9181 acttgatggt ataaaagatg gagtaattgc tatagatagt aatggcagta taattttgta
     9241 caatgaaagt gcaaaaaaca tgcttaaagt agagagaaaa tgtgcactta ataaatacat
     9301 cttagatgta tttcctaaaa tggagtggat gttagattgt ttacatgaaa aagaggacgt
     9361 agaagataga aagataagaa atataaataa tctaattgtt aatactagga caacattgat
     9421 aaaagttgat aatagtactt atggagtttt aggaataata caagatataa ccaagctaca
     9481 aaacttagaa agaaaaataa gatttgactt gaatcaaaaa ggtctctatg ctagatatac
     9541 ttttgatgat tttttattta aagataaatt aactaaagaa tttattgaag aagctaaaaa
     9601 aattggaaaa agtgattata caactttact ttatggggaa agtgggtctg gtaaagaaat
     9661 tatagctcat agtatacaca atataagtaa aagaaaagat aggccttttg ttgctataaa
     9721 ttgtgctact atagcagaaa atttacttga aagtgaactt tttggctatg aagaaggtgc
     9781 atttactggt gctagaaagg gtggaaaaag agggctattt gaacttgctc atggagggac
     9841 tcttttttta gatgaaataa atagtctatc ctttaatatt cagacaaaac tattaagagt
     9901 aatagaggaa cgtcaaataa tgagaattgg ttctgattat ataatacctc tggatataag
     9961 gattatagca gcaacgaacg aatctttaac cgaaaaaata gttatgggta catttagagc
    10021 agacttattt tatagattga gtagtttaga gataaatata cctcctttaa gagacagaag
    10081 agaagatata atacctttgt ttaacaattt tgtaaatgaa gtattaaagg atgatggtct
    10141 caatggaata aacagtatag atgaaaattt tgttttgact aaagatgaaa tggataagct
    10201 atataattat agctggcctg gaaatgtaag agagttaaag acaatagctc aaaaatatgt
    10261 tgtgacaggt aaaattaaat tgagacaaga tagagatttt aaaactaaaa aattacttcc
    10321 taactctgaa gtagacaaat ttaattctga aacgacagca agtgtagaag tccaagatga
    10381 atctataaat ataagtaaga taaatgatgg taagataagt atagatataa aagaagtaaa
    10441 taagtatgtt gaagaaaaaa taataagtat gctttttgct caaggacttt ctaaaaatga
    10501 agttgcacaa gttcttggaa taagcaggac atctttatgg aagaaataca ataaaaatat
    10561 ttaaatataa ttacatataa attattttga agtaatgtta attttttaaa acaagtgtta
    10621 agaaaaatta acattacttt tttgcaaatt tttatttttt tagacaggca tatatattta
    10681 taagaaaaat agttaaaaca cttaatttaa taatgaaaaa aataataatt ctaaacaaaa
    10741 tcaattttac ataaagtggg atttattggc atagtaattg cttaatatat aatgtaagag
    10801 taaatacaat tttaggggtg atggtatgag tcaaagtatg gttggtaagc atgcaatgtg
    10861 gccaaaagaa aatgatgtta tatttagcat atctggcaga gctcaagcag cagagaaggc
    10921 ttttggtatg gataatgtaa taaatgcaac aataggcgct ctaatggatg attcaggaaa
    10981 attgataaca atgaaaactg tatatgaaga atataaagca ttagataact gtgagatagg
    11041 tgcatatgca gctcttgaag gtcaaccaga ttacttagaa gcagttaaaa aagtattttt
    11101 cagagattac ttaccagaag gacatataag agtattagct tcaccaggag gaagtggtgc
    11161 aataaaatta gctgtatgga actatactaa tgaaggagat gaggtattaa catctgattg
    11221 gttctggagt ccatatgtaa gtatagctga agaggctaat agaaaagtag ttaattacca
    11281 actttttgat gaaaatagaa ggtttaattt tgagtcgttt aaagaaaaat ttgtaaatat
    11341 agcagaaaaa caaggtagag tctttacaat tataaacaca ccagcgcata atccaactgg
    11401 atatagtgtt gcagatgatg agtgggataa aatattagac ttatcaaaag aagtcgcaaa
    11461 agataaagac aaaaagataa tatttttcgt agattcagca tatattgact ttgcaggtga
    11521 tgatgatgta tgtagaaagt tttttaagaa attttctaat ttgcctgaaa acgttctcgt
    11581 attggttgga ttcagtatgt caaaaggatt tacagcttat ggtatgagaa tgggggctat
    11641 aatttgtata tcttcaagtg aagatgtagc tgaagagttc cattactcat gtgttcattc
    11701 atgtagagca aattggtcaa attgtaatag aagtgccatg gcagtattat ctaacattgt
    11761 aaatgaccct aaaaaattca aagaatatga ggatgaaaaa gaaatataca aaaacatgct
    11821 tactagaaga gcagatgtat ttgtaaaaga agctgaaaga gtaggattag aaattcttcc
    11881 atatatagca ggtttctttg taagtatacc atgtgataat ccaaaagaag tatgtgaaga
    11941 attgactaaa cataatttat ttgcagttcc attaaaaatg ggtttaagat ttgcagtttg
    12001 tgcagtttct gaagataaat gtaagaaagc accaagtatt ataaaagaag cacttgaaag
    12061 tttagaagta aaaataaata attaatttca ttgaacaaat tgggtggact atgaatttaa
    12121 taattgtaga tggagggaat tttatatgaa acaattgatg acaggtaatg aagcaattgc
    12181 acgtggagcc tatgaggctg gtgttaaata tgcatctgca tatccaggga ctccaagtac
    12241 agaaatatta gaaaatatag caacatataa agatgcaata gttgcagagt gggctccaaa
    12301 cgaaaaggta gcacttgagg ctgctatagg gggttcaatt gcaggagcaa gaacaatggc
    12361 ttctatgaaa catgttggtt tgaatgttgc agctgatcca atatttactt atgcatatac
    12421 aggggtaaat ggtggtatgg tacttattac agcagatgag ccgggaatgc attcatctca
    12481 aaatgagcaa gacaatagga tgtatgcaaa atttgcaaag atacctttat ttgagccatc
    12541 aactagtcaa gaagctaaag atatgataaa agaagctttt gaagttagtg aaaaatacga
    12601 tacaccagtt ttatatagag ttacaacaag actttgtcat tcaaaaggat tagtggagtg
    12661 ctatgataga gaagaagttg aaattaaaga gtatgttaaa aatgctaaga aaatggtaac
    12721 tgttccagca aatgctcaaa taagaagggg cgttgttgaa gaaagaatgg aaattcttaa
    12781 aaaattctca aatgaaactg atttaaacta ctatgaaata aatgatacaa aaataggtgt
    12841 tatagcctct ggtatgtgct gtaattttgc caaagaagtt tttggaaaaa atgcatcata
    12901 tatgaaacta ggatttacaa atccattacc ttatgaaaaa ataaaagaat ttgcagaaaa
    12961 agttgataaa atatatgtta tagaagaaaa tgacccattt atagaagaac aaataaaagc
    13021 atatggaata gattgtattg gaaaagatgt aataccacca tatggagaaa tgacaccaga
    13081 tgttttaaga aaagctatat ttggaaaaac aaatgatact atagaataca aatctgaatt
    13141 agtaacacca agaccaccaa gtttctgtgc aggatgtcca catagaggat tcttctatga
    13201 acttggaaag agaaaaaatt taatagttgg tggagatata ggttgttata ctcttggatt
    13261 tgctcctcca tataatggaa tagattatgt tgtttgtatg ggttctgctt ttggaactgc
    13321 acatggagct caaaaagttc ttaatatgaa ggatgataat gaaaaaagat tggtaggtgt
    13381 acttggagac tcaacattct tccatacagg aataaatgga cttttagatg ttgtttacaa
    13441 tagaggtaat tctatttcgg ttatattaga taatagaata actggaatga caggtcacca
    13501 agaaaaccca ggttcaggat atactttaca aggtgctaaa actaaagaag ttgatataga
    13561 aggattagtt aaagcttgtg gaatagaaca tgtaagagtt ataaacccta ataatttaaa
    13621 agaagtaaat gaagcactag attgggcttt agctatagaa gatgaaccat cagtaataat
    13681 aacaagatgg ccatgtgtac ttaaaaaatt ctctaaagaa gatatagaag aatttaataa
    13741 tccatttaag actaagtgta aagttgacca tgataaatgt ataggatgta aattatgttt
    13801 gaaaactgga tgtccagctc tatcatttga taaagagaat aagttatcaa atatagatag
    13861 aaatcagtgt gtaggatgtg gagtatgtgc acaagtttgt ccaaaacaag caatagttaa
    13921 ggaggaaaaa taatatgact aagagtatac ttttggtagg tgtaggaggt caaggaacta
    13981 tacttgctag taaattatta actatgggat tgatggaagc tggatatgat gtaaagatga
    14041 gtgaaataca tggaatgagt caaagaggag gttcagtttc ctcacaagtt agatatggag
    14101 attgtgttca ttctccagtt atagaaattg gtggagcaga tatattagta tcttttgaaa
    14161 aaatggaagc tttaagatgg ttcaattatt taaaaccaga aggaaaagct ttagtaaata
    14221 atcataggat agattctatg actgttttaa tcggaggagc tgaatatcaa gaaagtgata
    14281 tagatgcaga gttaagtaga ttaaatgcaa aagttataaa tgcagctgat aaagctgaag
    14341 aactaggaaa ttcaaaaata atgaatgtta ttttattagg atgtctagtt aaatctatgc
    14401 aattagagtc tattgattgg gaaaaaatta taagtgaaaa tgttaaacca aaatttgttg
    14461 aattaaacat aaaagctttc catgaaggta tggaaatggt tagcaaatag tttataatag
    14521 atataaatag actatagaaa ttaaggtgaa aggaagaaaa ttatgactta cagaatatta
    14581 gccataaatc caggttctac ttctacaaaa atagcagtat atgatggaga agaacaaatt
    14641 cttgtgaaga cgatagacca tccagctgaa gagattgcaa aatataatac tatacaagac
    14701 cagtttgaaa tgcgtaagga agcagttttg aatattctta aagaaaatag tatagactta
    14761 aaatctctta gtgcaatagt aggaagaggt ggagttttac caccagtaaa atcaggagca
    14821 tatttagtaa atgaagaaat gattgatgta ctaagacata gaccagtact tgaacacgct
    14881 tccaatttag gtgctgttgt ggcacatgca atatcagaac ctcttggaat caactcatat
    14941 atttatgatt ctgttgcagt agatgagctt atagatgtag cgagaatatc tggactttgt
    15001 ggaatggata gatcaagtgc agggcatgca ttaaatacta gagcaatggc tttaaaatat
    15061 gctaaggata aaggaaaaga ttataagagc ttaaacttaa tagtagctca cattggtgga
    15121 ggagtaagta tttatcttca tgaaaaagga agaatggttg atatgctatc tgatgatgaa
    15181 ggaccatttt ctccagaaag gtcaggaaga gtacctgcta caaaattagt ggctgcctgt
    15241 tattcaggtc aatattcaga aagagaaatg actaaaaaga taagaggtaa aggtggtata
    15301 gtttcatacc taaatactgt agatgctaga gaagttgaaa aaatgatagc agaaggaaat
    15361 gaagaagcaa aaattattta tgaagcaatg gcttatcagt tagcaaaagg tattggagag
    15421 ttagcaactg tagtagatgg aaaggtagat gctataatta taacaggtgg aattgcatat
    15481 tctgaaatgt ttacttcaat ggttaaaaag aaagttgagt ttatagcacc agtagaaatt
    15541 atggcaggag aaaatgagtt ggaatcactt gcttttggaa ctttaagagt actaaatgga
    15601 gaagaagaag ctagaattta tagtgaaaat taataagaaa aactaaaaat gtttatatac
    15661 taaaaaagtg gtatatccgt tttttataca cgaaagctta gagtataggt gaaaatgtat
    15721 aaaatttgga ttgccacttt ttataattaa aattttatct taacctaata aataattctg
    15781 taaaatttcg tatttatata tatattttat tggtatttat tgttattatt attatgagga
    15841 gtgatttttg atgggaaaaa taagtttaaa aagtatgata agattatttt caggtttttt
    15901 tatatttgca atttcttctg tgcttatgat taatgcacat gtaggtctta tgccttggga
    15961 tgtacttcac caaggtttat ctataaagct aggcataact ataggtcagg cttctataat
    16021 ggtaggagtt gtaattgtta tattggatgc agtttttggt gaaaatatag gatggggtac
    16081 tttactcaac atgacattta taggaatttt tattgattta gtaatatttt ctggagtgat
    16141 acctcatgca agcaacactt atataggtgt gtttatggtt gttataggaa ttattttggc
    16201 agctatagca agttttctat acttaggtgt ttgtttagga agtggaccga gagatgggct
    16261 tatgattgct cttcagaaaa aaactaataa atctgtacgt cttgttagaa caattcttga
    16321 aatactagca ttagttgttg gatggttatt aggaggttct gtaggtattg gtacgcttgt
    16381 aagtgcttta ggtttaggat atgtattaca aatagtgttt agaatattta aatttgacac
    16441 aaaattgctg aaacatagat ttattataga tgatataaga gaatggaagg aaaagaaaag
    16501 caatgaacat aaatgtaaat caagtattgt aataaaaaat gagcagaatt aattatgcta
    16561 aaatatattt gtagtttatc taatttatag taaagtcaag gagattttga aatggaatta
    16621 tgggatttat ataatgctga tggaattaaa acaggaaatg ttataaaaag aggtaattct
    16681 attgaagaag gttattacca tttggcagtt gaagtgtgga ttttaaatag taactctcaa
    16741 attttaattc agaaaaggtc taaaagtaaa aaaacattgc caaatatgtg gggaatgaca
    16801 actggatgta ttgtttctgg ggaagacagt ttagaaggag caattcgcga agcaaaggaa
    16861 gaaataggga tagatataac taaagatgag atgaaagttt ttagaagtat gattcatgaa
    16921 gatactcttt gggatgtata cttagttaaa aaagaatatg atatatctaa agctatttta
    16981 caggaggaag aagtaagtga tataaagtgg gtaagcacag atgaaataag acaattacta
    17041 aaagagggtt tgttttttga atatccagag atatacgaat tgctatatga gatagataat
    17101 aataaattaa atatatgatg tagagcagat ttcagtataa gtaagattat ttaaaatgta
    17161 tcttctctaa attttaatat tgttttatga aaattaataa gttattttta gtatttttct
    17221 acttgttttt tgagattaaa ataaaaaggg cagtcattat aaagtcataa aaatttgggg
    17281 aggatttaaa ctatggaaga taacaaagga ttggcacgtg acagttcaaa atataaaatt
    17341 gtttctatat ctgtcgtagt aattattcta attacatttt ggtatatgtt aaatatggtg
    17401 cttttaacat ttattatgac ttttattttc tataacttgc ttgtagccac aagaaaacga
    17461 attaagaagt tttcttcttt aaatattcca gattcattga taattatagt gttatatgca
    17521 ctatttgcta tattgttagt attgattagc tatgcagttg taccaataat tatagttcaa
    17581 cttacagagt tgagtagggt ttttagtgat tttgatgtaa atcaatttgc tcaatcttta
    17641 ggaccgaaat tgtatcctat tgtctcaaag ctggatttta ataaatatat ttcacaagca
    17701 gggctactta tagcatcaac agcaacaaaa gttggaagtt ttggagtaaa tatattattg
    17761 gcatttttac tgagtttact ccttttactt gaaaaaaatg aaattaaaaa ttttggagat
    17821 aaactttctg atagtaagat ttcttttatt tataattcgc ttgtattttt tggaaagagc
    17881 tttgttaaaa attttggtga agtaatgaaa gtacaagtta tgattgcctt tataaattct
    17941 gttgtttcta tgattttctt aggtttcatg ggattccctc aaatatgggc tttaggattt
    18001 atgatttttg tgttgggtct tattccagta gcaggagtta tagtgtcttt gattccatta
    18061 actgtaatag catttaatac aggaggaata acaaaggttt ttggagtttt acttatgatt
    18121 tgtatagtgc atgcagtaga aacatacata ttaaacccta agttgatgtc gaatagaact
    18181 aaacttccag tttgttttgt ttttattatt ttacttgtag gagagcatta tttaggtgta
    18241 tggggattac taattggagt tccaatattt atgttcctta tggatattct gggtgttaaa
    18301 tttacttcta ggtaatatat taaaataata atttattttt atatataaat tatgaattgt
    18361 tttaaaaaag aacctataaa tagacatcat taataaagac atctatttga taggttcaat
    18421 tttgctttgt ataaatacta taaaactaaa taaaaagaaa tttatttcat ttcaaaagtt
    18481 tcacaacttg catttctttc atttatatga actttttcag ctttgcaaag ttcattttta
    18541 ttgtatttac attttacagc ttcacaatga atgttatctg tatcaacttg gttagtttca
    18601 ttagaacgac tagttatacc agatgtatat ttgtcttcaa aagaagcaca ataagtattt
    18661 tctgtagtag ttgcttgcat tccatctact tttatactac cagcataaca atatccacta
    18721 gtattataag cacaattagt agcagcacaa tttaaattat tatttttcat aaataacctc
    18781 ctaagtttta tttaaactta ttatgtgaag ttatctaaat tttatacact tatttacaat
    18841 tacatttatc acatttaaca actacatctt tggaagtttc ccttcctaaa tttttattaa
    18901 catgatacac atcaacattt tcagttgatc cacatataga acatttaaaa ttgttttcta
    18961 gtagggttct ttctttaaat aacttccaat acatagaatt gatgtaatca ttataattta
    19021 ccttgaggca tgtctcaaga aagtttatgt atagattgtc taaatctcct gtatttaatt
    19081 ctgtatattc tggttcaaaa tattcacaac atcctctaaa atcgggcttt gtatctgaaa
    19141 taacacatct aatatcatat ctatagtcta ggctaggccc cttaaatgaa agatgtgtgc
    19201 aaaaagaaca agatatttct ggtagatttt tttcaagttc atgtaattta ttattcataa
    19261 gtattcctcc aaaaaattta ttttaagtat ataaatgaat agattatttt aaaactttta
    19321 tttatttata ttagagattt aggataaata ttccaataag gagtaaatac ttaagtattt
    19381 attactgtat taaataaaaa agaggcaaat ctaacttgag attgcctact tttttatcat
    19441 tttttaataa aatttattct aagtattatt ttatttagat ttagtcagcc aacttagtat
    19501 ctgctttgct ttttaacttt ctaccagagc gtacagttaa aatagcatat aatacagtaa
    19561 atatggcagc taaaacatat gatatattta tgtttaagtt aaatccaatt tgagcattaa
    19621 atatataact gaatataaca aaggaataaa acataccagg aattaagctt ataatataat
    19681 tcttttcttt tattataagg taaactgtaa tcattgcaaa tgcaaatatg gcaatagttt
    19741 gattactcca agagaagtat ctccacaata tattaaatcc agaagcacta agctttgcaa
    19801 atataaggat ggctatgaca ggaataaata tacagattga caatataaca cgatgtttct
    19861 tttccttttg gtcataatga aagtaatctg caagcattaa tcttaaagat cttaaagctg
    19921 tatctccaga agttattggt aaaacaatta cacctattat agctatgata ccaccaatag
    19981 aaccaagtaa atctcttgca accaaaccta ttacatcagg agaacctact aattctttag
    20041 gtattccttt attgtaaata cccatagcag ctgcagccca aatcattgca attaaacctt
    20101 ctaaaatcat catattataa aaagtagttt ttccttcttt ttcatttgaa actgatcttg
    20161 ctataagagt agcttgagtt gagtggaatc cagaaacaat accacaagca actgtaacaa
    20221 agaatgtagg aataagagga ataccatcag gatgaatacc tttccaattt gcaagactta
    20281 aattagctaa atcataacct tgtgtaaaga ttccaattcc aacaccagca gcagataaaa
    20341 gtagaatagc tccaaatatt ggatatacct ttcctatgat tttatcaatt ggaaatagtg
    20401 ttgcacacaa gtaatataga aagataagtc cataaactat ccagacaact ggattattta
    20461 tagtcgattt catatttaaa atttgagtaa caactaaatc cccaggtgta taaataaaaa
    20521 ctgcaccaac taaaagcatt aaaagacaaa ggaatatatt atagacttga aatactttat
    20581 tacctaaaaa tctactgata agagaaggca tttgtgctcc tttttctctt atactaatca
    20641 ttccactcat atagtcatgc aaagctcctc caaatacaca gccgataggt attagtatga
    20701 atgctattgg accaaacaat ataccttgga taggtcctag aattggtcct gtaccagcta
    20761 tattaagcaa ctcaattaga ctatttttcc attttttcat tgggacatag tctactccat
    20821 cactttgagc aagtgcaggt gtttttctgt catctgggcc aaatactttt tcacaataag
    20881 caccatataa agcacctcct attagtaata tagccaagcc gattaagaat gttgtcataa
    20941 tatcacccca tttataaaat tttttggtct ttgtatacat attatctaaa tgtgtagatt
    21001 tatattgaat ttaattctgg acaacctaca aattctataa agtattacaa ctaatataat
    21061 ttcagaaata aatacatata cataaccatg tacttagtat acaggtattt ggtaatattt
    21121 tcaatgttat agcaataaag aatataaaca aaattctgat aatattttac aaaaaaatta
    21181 aaaaatataa aaattagttt aaatagatgg agaaaaaagg aaaatcaatt gagaatataa
    21241 atgtctatat cttaacaata ttaagaggtt taaaattctt gaagaaagaa tagtcaagtg
    21301 atataatggg tttacaggtg tatatacact agtacatata gaaagcggtg caattatgga
    21361 taaagattta acatatcaaa tcaaagagct aaagaaagaa aaaaatgcaa taatacttgc
    21421 ccatttttat caaccaccag aaatacaaga attagcagat gcagttggtg actcatatta
    21481 tttaagtgag attgctagag attgtaaaga agaagtggtt gtattttgtg gagtaaggtt
    21541 tatgggagaa agtgctaaga tattatcacc tgaaaaaact gtattgatgc cagtaagtaa
    21601 cgctgggtgt gctatggcag atatggtaga tgaagaaggc gtgataaaat taaagcaaca
    21661 gtatccaaat gctttggttg tatgttatat aaactctaca gctaaagtta aggcacattg
    21721 tgatgtttct gttacatctt ctagtgcaat taaaatacta gaaaacatag ataataaaga
    21781 aattatattc cttccagata aaaatttagg aggatatatt gcagaacaat ttccagataa
    21841 aaactttata ttttgggacg gatattgtaa ataccataac aatataagag ctgaagaaat
    21901 aattgagtta aaagataagt ataaaaatgc agaagtatta gtacatccag aatgtaaaaa
    21961 agaaataaga gatttaggag attatgtagg tagtacatca ggaataataa aatatgctac
    22021 taatagtaaa aacaaagact ttataatagc aactgaagaa ggtatattac atgaattaaa
    22081 gaaaaataat cctaataaaa acttttatat tcctggtgga aaaatactat gtacagatat
    22141 gaaaaagaca acattagaaa atttatatag tacacttaaa aatatggaaa atgaagttat
    22201 tgtagaagat gaaatcatgg aaaaggcttt aaattcactg cttaacatgc acaaattagc
    22261 agaaggatag agggatagac ttatgaattt agaacaagat gttttaatag ttggaagtgg
    22321 agtttcagga ttatactgtg cattaaattt agataaaagt ttaaatgtgc tagtagtatc
    22381 gaaatcaaca attgaaaaca acaatacata tcttgcacaa ggtggaattt caactgctag
    22441 aaatattgat gatatagaat cctttgtaga agatacaatg aaggctgggc agtataaaaa
    22501 tagagttgaa gcagttcaag tacttgcaga tgaatctata gaaaatgtag gtcagatagt
    22561 agaatatggt atgcctttag acaaagaaaa tggagagata gattacacaa gagaaggagc
    22621 acatagtgta aatcgaatag ttcatagtaa ggataatact ggagaagttg tttttaaaac
    22681 tttattaaaa gaagctaaaa ctagagaaaa tataactctt atagaagatg catatctttt
    22741 agatatatta aaagatggca ataaatgtat aggagctaga atttttaaat ctaaaaaaga
    22801 gattcatgtt ttttcaaaaa tagtagtttt agctacaggt ggaattggtg ggctttttaa
    22861 aaattctact aatcaaagac atcttacagg tgatggaatt gcaatagctt taagaaataa
    22921 tataaaaata gaaaatttag aatatattca aatacatcct acagcctttt atgaagaaaa
    22981 taatgaaggt aggagaatgc ttatatctga atcattaaga ggagagggag ctaaactttt
    23041 aaataaaaat aaagaaagat ttgtggatga acttcttcct agagatgtag tttcaaaagc
    23101 tatttttgag caaatggaaa aagataaatt accttatgtg tatttagatg caacacattt
    23161 agatagtgaa tatttaatta atagattttc ttttatttat aatgaatgtt tagctagagg
    23221 aacagacata acgaaagaat gtataaaggt atctcctgca caacattatt ttatgggcgg
    23281 gatacatgta gatttggatt caaaaacatc tatggaaaat ttatatgcag ttggtgaaat
    23341 tagttgtaca ggtgttcatg gagccaatag attggcaagt aattcattac ttgaagggct
    23401 tgtattttca aaaagagcag ctaaaaatat aaactcagtt atagataatg taaaagttaa
    23461 gtttatagat gtaccagata tggatataga tatagaacag gtaaaaaaag aaaataaaat
    23521 attagtcata aaagaaattg aaagaacaag tgaggatttt ggcgatgaat tatttgatta
    23581 ttgatagaat gatacaggat gctttgatag aagatgttcc aagtgaagat ataacaacta
    23641 attctattgt ggatgaaaat tctaaatcta ctgtagattt aatttgcaaa caagatggta
    23701 taatagctgg cttaggtgta tttaagagag tttttgagat acttggtgat gtagatgtaa
    23761 aattatataa aaatgatggt gatagagtaa agaatcgtga aaagatagct gtattaacag
    23821 gtagtactag aaacttactt gtaggagagc gtgtagcatt gaattattta cagagaatga
    23881 gtggtatagc tactattaca agtaggtatg ttgaaaagct tgaaggtaca aatacaaaat
    23941 tattagatag tagaaaaacg atacctaatc ttagaatatt agataagtat tctgtcaaag
    24001 taggtggagg atgcaatcat agatttaatt tatctgatgg aatattgctt aaagacaacc
    24061 atattggggc tgctggtagt gttaagaagg cagttgaatt agctagaaaa aatacttctt
    24121 ttgttagaaa gatagaagtg gaagtggaaa ctttagacat ggtaaaagaa gcaatagaag
    24181 ccaatgcaga tataataatg ttggataata tgagtttgga aatggcaaaa caagcagtag
    24241 atattataaa tggaagagcc attgtagaat tttccggaaa tgtaaattta gatactatag
    24301 aagatatagg aaaaattggt gtggatgttg tttctgtagg agcgcttaca cattctgtta
    24361 aagctctcga tataagtatg aagaatttga gaaatatata aattttatat tgaaataaac
    24421 ttaattaaaa tagactcctt aaatttgttt tataaattta aggagtctat tttttattca
    24481 ttaaaattaa taaaaataga aaaaaatcaa tcaataattt aataaaaata gataaatact
    24541 atatacactt ttaataatat agttaatatc tattttacag ataaataaga ataaacttat
    24601 aatataattg tatctgtaaa attttgtaaa atataaaaag tataggggga ataaaaatga
    24661 aagtagctta tatttttagt agtactaata cacataaaat tctagacaaa atgataatac
    24721 cacaacttga acaaggaact catggagtag acgttctagg gatgtttttc tttatggata
    24781 ataccttatt tttatctaaa ggtaatcctg taggcgagag actaagtaaa ttacatgaaa
    24841 aaactgatat gataataatg gcttgtgacc aatgtgctat agaaagaggt atagagaata
    24901 atttggttga tggagctaca attggatgtt tcccaaacct atatgcttgt ttaggcagtg
    24961 caggtgtgga ccaagttata acgttataaa ttagtatagg atacaatcca tagaaaaatt
    25021 tattttctgt ggattatttt aagtaaaaga taaattgttt aaagtagggg atgaacaaat
    25081 gtctaaaatt ttagttgaat ttataaacac ttgaccatct tgtgatgaac atggagtgtt
    25141 tataaaaaaa atgtcagaaa agtatgctga taaattggaa ataaaattat atcaagcagg
    25201 gaaagatttt tcgtatataa aaaaatatgg gattataaca aaaggaactt taataataaa
    25261 tcagaaaaag aaatatgata gattaaataa agatacaatt gaaagggcaa tagtagaagc
    25321 tataaacaat aattaaaagt ttaaaaatta ggagtatcaa aaatgaatat attagaaatt
    25381 gttttaaatg gagttaaaga tactatagta gcttctatag ttacactgaa tggagcatca
    25441 ggatggctta tgataagttt tatcttggca ggtgtacttc acaatgtttt aactccaaga
    25501 aggtttcaaa aacaacttgg aaataagaaa ttttcttctt taattaaaag tactatctca
    25561 ggaatgtttt taccaatatg tagttgtgga gtaataccac ttggaataag tatgtattat
    25621 tcaggagcat atttaggacc agtacttgct tttatgacat caactccaat aataaatcca
    25681 atagctgtag ttctttgtct aggtctttta ggtcctaaag ttacaatcat ttatctaata
    25741 gctggttttt tagtaccatt tttagtagga tttttaggaa attgttttgg aaaagaagaa
    25801 ttatcacttc ataatgattc agaggaagaa gtaatagaat tagaagaaga aaaaagaagt
    25861 tttatggaaa atatgattga tggtcttaaa tggtcttttg gtgaattagc ccttactatt
    25921 agtaaatatg tagtaatggg tatggttttt gcaggattta taacaactat ttttccaaat
    25981 agtataattc aaaaatattt aggaaatcca ggtttgctat ctcttggaag tatagcaata
    26041 cttgcatgtg ttatgtatgt atgtgctgta ggacatatac cgtttatagc agcccttgtt
    26101 gcaagtggag catctcctgg tatagcgata acatttctaa tagctggagc agctacaaat
    26161 ctaccagaac ttataagtat gtttaaactt attggaaaaa gaactgttat tatatattct
    26221 gtaactttga cactgtcttc aataataatt ggatatataa caaatttact tttagtgaat
    26281 aataactcag taaatttaga tgcaacaaat tcttcaatac agttggcaaa taaactaatg
    26341 ttaaatatac ctgatagttt tgagtatgta tgtactattt taattatttt atttgcattt
    26401 aaatcaataa taccaaagat gaaagaggtc ataggaaatc atgcttaaaa agatagttat
    26461 aatatcctgc atagtagttg tgttaatcat tttatcaaaa atagttgatg ataacatcaa
    26521 agaagatgct tctattccaa atgtgaataa agaaacatta gaatatttta gaaaaaatta
    26581 taaagaagac ataataactt gtgctgaaga agacttaaat aatgatggga aaaaagattt
    26641 agtagttata tataaaaaaa gcaataattc aaatgaaatg gtagtcgtag tttctgataa
    26701 aaatagccac tatataacaa agccaatacc agcacctata gagaatcaaa ctataacctt
    26761 taaaaatatt gatgataaag caccaataga agtaattgta tcagggtcta aaaatggtaa
    26821 tgtaggatat gctatttaca gagtagaggg taaaaaattt gttgatttat ttggcgaaga
    26881 tatggacaaa tgttgctaaa aataaaaata atacataaag gtgggaaatt tatatgaaat
    26941 tatctaagaa gattcaagca ttaatcttat ttggtgtaat gggtacatct atattaacag
    27001 gatgtagtag taaaccaaaa gaaaaagaag aagcaaaaac tggaaaagat gattatacaa
    27061 taacagtagg ttattataat tgtgaccata tgactgcggg accagtagca gaatcggcag
    27121 gaatatataa agatttaggt cttaatgtaa aaactgttgg aaatggtaaa gttcctgaag
    27181 ctatggcagc aggaaaaatg gatgcaggtt acataggaac taaggggtta gttggagcaa
    27241 tacctaaggg ttcaccaata acaatagcag ctaacaacca tactggtggt tctgaatatt
    27301 taatagtttc aaaagatata aaagaaccta aagatttaat aggtaaaaaa attgcaacag
    27361 atatgagtga tttcttatgg acaagtgatt atggtccaga aacaggtcta ccaactgacc
    27421 catcaaaata tgaagttgta aatatggatt cagacaaaga taaatattta gctcttaaaa
    27481 ctgggaaaat tcaagctttt acatcttgtg acccttgggg ttctgtagct gaaaatgacg
    27541 gtgcaggaaa aataatagca tctactcaat ataaagaaaa agctaatggc aaagaatata
    27601 actgttgttc attctcatta aacaaaaact ttataaaaga acatccagat ttagcaaaaa
    27661 aattggttct agcacataca aaagcaatag aatatatata tacaaatcca gctgaagcag
    27721 ctaaaatatt tgcaaaatat tataatgtag aagaagaagt tgcacttaga actatctata
    27781 agaagactgt tggagaaggt agaactctta cttggaaagt tacaggtgaa gaatataaaa
    27841 ataatttaca aatgtataaa gatttaaagg ctttagatga tatacctaaa tatgaagata
    27901 cgattgatac aagcttattg gactcttgtg gagctgatga ttttgacaaa tttataaaag
    27961 aaaaagttaa cccagagttt cctgaaggta tgagttatga agactggaaa gcaaaggtat
    28021 tataaataaa tggagaatat gaaaaatata ataatacttt ttatcattat tttgggaagt
    28081 atatttataa tattttttaa ttttgaagat aaagaagaaa taaaaggaaa attaagaata
    28141 ggggtatctg atgatacctc tggttttgta ataaattata tggtaaatag agactacttt
    28201 gaaaatataa aagtaagtga tataatggat atttttacaa ttaatgattg ctgagcaagt
    28261 acaatgcagt gggctctgag ttcagaggaa atagatatag caatcatatg taaagatgct
    28321 gcaaaacaat atgtaaatgt aaatagtgga tttgaaattg ttggtactgt agttcaaaat
    28381 tctgatatat ttctattaag tgataataat cctgaaaaga taggagtcat tcaaaataga
    28441 gattaccagt cagaattggt taaaaaatac tacaagaatg tggaagtagc tcccttatta
    28501 gggactgcac tagcatatgg attagaaagt aatttagtag atggtgttgt aatagattgc
    28561 ataaagtcaa ttgggttaga gggcaaaaga aaatctacaa ctaaattagg agattatgat
    28621 acctatgtat tagttgtaag taaagagttt aaaaaaagta aagcatatag tgattttata
    28681 acactttaca ataaaagtgt tgatgcttta aagaataaag ctacatttaa gaaagcatta
    28741 aaagagtaca ggaatataag aaatatagat atggaggaaa taagcaattg gaaattaaaa
    28801 tttctaaaga taaaagcgat agattaacta agaaaaaaac tgatggtagt atggatattt
    28861 tatataaaat aatactatta gttattatat ttttagtctg gcattttgca gcaaaagata
    28921 tagggagttc tttgttactt ccaatgccag tagatgtaat aaaaggtttc tttttctgtg
    28981 tgacagatgc agagacagtc actaatcttt ttataacatt gcagagagta cttaaaggtt
    29041 ttatgtatgc attattgttt gggcttccaa taggatttat tatgggattt tctaaaactt
    29101 ttgaaagggt tttatctcca gtagtagatt ctgtaaggca agttccaata atggcatggg
    29161 ttcctcttac tatagtatgg tttggtatag gagatggtcc aacaatattt ttaatagctt
    29221 tttcaggggt tttccctata attttaaata ctattcaagg agtaagagca atatcaaagg
    29281 attattataa tgcagctaga agtatgggag catcgccaat tgtgatattt acaaatgtaa
    29341 ttgttcccgc atctcttcca gatatactaa ctggttcaag gattgctata agtacaggat
    29401 ggatgtctgt tatctgagcc gagtttatag ctacgagtgc cggtctcggt tactcaatgg
    29461 tacaagctca aacattaatg catacagatg tattaattgg gcttatgata tttgcagctt
    29521 taataggatt tataatagat agggtattaa agtttataaa taaaatcctt tgtagatgga
    29581 ggtttgcaga ttaatgaagt taagtgtaag agatataaat aagacatttg taaataatag
    29641 agtacataca aaggttttag aagatataag tatggatata gatgatggac aatttgtctg
    29701 tcttttagga ccatcaggtt gtggaaaaac aactttactt actataatag gtgggtttca
    29761 aaaatcagaa cgtggagatg tttttataaa tgataaaaga gttaaaaaac cgggcataga
    29821 tagagctttt atatttcaaa attatgcttt atttccttgg aaaactataa gaggtaatgt
    29881 tctatttcct atgaaacagc agaaaatacc aaaagaaaaa agagaagaaa tgctagaaga
    29941 acttttggtt atgtcagact tgaaaggaaa agaaaaactg tttccgcatc aattatctgg
    30001 aggaatgaaa caaagagtgg ccatgataag agctttagca tgtaatccag aggtattact
    30061 aatggatgaa ccattaggag ctgtagattt tcaaatgaga caaaatttac aggaagagct
    30121 agaacgaata tggataaaga agaaaataac tgcattaatg gtaactcatg atgttgatga
    30181 agctgtatat atgagtgata gagttatagt aatgtcgaga gataaaggaa gaattattga
    30241 tgatataaat atagatattc caagacctag aattagagga agccaaaaat atgaagagta
    30301 caaaaacaaa ttaactgata ctttatcaaa gtgttatgaa gtttaaagag gagtagacat
    30361 gagtcaaata gaaaaataca atagtttgga taatacacaa cagaaaataa gaatctctat
    30421 tatggatatg ataatagata aaggttctag tgttacactt caagaagtga ctgaatatat
    30481 aagtaaaaag ttgaatatag aaaaagaata tatagaaagg acactacaat attttattta
    30541 taaaaatata atggttgttg atgggaatag tataaatttt atatatcctg tatcagcatt
    30601 gccaacaaat cataaagtaa ctttgagaga taatagaagt ttcagtgcaa tgtgtgctat
    30661 agatgctata ggaacctctt gcacattcaa ccaagatata aaaataaact ctatttgtag
    30721 tgctacaggt aaggaaatag aagtaattat aaaaaatgaa aagattgagt atgtgaataa
    30781 tccaaactta agagtgcttc atataaatct tgataagcat ctaaattggg ctgctagttg
    30841 ctgaaatata atgaatttct tctggacgaa gagtgatttt gatgcttgga caaacgaagc
    30901 gggattatct aatgatgaag atatatattg tttagatatt aatgaggcaa tagtagaatc
    30961 atataaaata tttaaactca aacaaaaggt actatcatag atatacatat atgaaaaact
    31021 aaaaatgacc tttagataaa aatgataggt aatatccata ctatataaat tagtatagag
    31081 tagggatgtg aaaagtattt aaatctaaag gtatattttt agtttttttt agatgattag
    31141 atagccttat taagtatata tttatttata acctttgcaa ctccattttc attattagta
    31201 tcagtaacat aatctgcaat ttcctttata ctattacgtg cattccccat agccacacca
    31261 agacctgcat actttatcat atgttggtca ttttcttcat cgccaacagc aatagcattt
    31321 tctataggta tatttagatt cgtacataat gttttaagag cagttccttt attagtagtt
    31381 ttacctaaaa attctagata aaaaggagca gaccttacta tagtatattt attgaataaa
    31441 tttttaggta gttgctgtat gatttcttcc aatctttctg gttcatcaac tatcataatt
    31501 ttacagattt taatagattc atcaatttta tctttttgta tatatttgac ttctatatta
    31561 tttaatttcg cttcatgaag tgtatatttg ccaggcgttt catttggtgt aattaaattt
    31621 gtcattgtat ttatgtgtat tttagtctta tattttttac aaacgttatt atatataaaa
    31681 tcaaaatcat ctaaactcat ttctacatca tgtatgattt tctcactttt aacttcttga
    31741 ataattgctc cattaaagca tataacataa tcaccttcat ctgttaaatt tagttgattt
    31801 agaagttctt ttacaccagg aagaggtctg cctgttgtta taactacttt tgccccagcc
    31861 ctttttgcct cctgaatgga ctcaaagact tcaggagtaa tccttttttg tgtatttaat
    31921 attgttccat caatgtctag tgcgattaat ttgtacatat caacctccaa taaattatat
    31981 actcaaattt cctgtgaaaa cgttgacaca gctagtgttg aacaaagttt cagtgggaaa
    32041 tatggtttgt tttgtatact attatatcaa gagataagta atttagaaag tttaataata
    32101 ttaatttttt gataaaaata ttacttatgt gagatgagaa tgtaaaaact ttgtaatcaa
    32161 taattccaaa attcttaatt acaaataatg gtatcgtttg cattgttaat ataaataaat
    32221 gtatattata tagttagaag tttgaataaa tagactgatg gtaatttgtt atattagtta
    32281 gttttgtgtt ataaaatatt gtaattccat taatcatatg ttattattca acatgattat
    32341 aatataaaga atatttgata tatataataa tttataaaag agtattacat agtacatgta
    32401 aatctatatt tataaataca ataaagtaaa gttgtaaagg agtgctttaa atgaagtttt
    32461 cagagtttaa gtatgaaaga cccaattata atagtatgaa aaaagagttt ttgagttgtg
    32521 tagaagatat aaataattct cgtaattata aagagcaaca aaaaaatata cataagatta
    32581 atttattaag aaataaaata gaaacgttgt caaatatagc ttcgataaga tatagtacag
    32641 acacatttaa taaattctac aaagaagaaa aaaattattg ggatgaatat atgcctttgt
    32701 atgaagaatt aaattcatat ttttataatg ccattgtaaa ttcaaaattt aaatatgatt
    32761 tgataaaaga gtttggagag caatttttca caatagtaga gtattcttta aaaagttttt
    32821 ccaaagaaat aataagtgaa ttgcaggaag aaaataaatt gtgttcagag tatactagac
    32881 ttttagcttc agctgagata atgtttgatg ggaaaataag aaatttatct ggtatgggga
    32941 aattcatgta ttctaagaat agaaaaacaa gagaattagc aaacaaagca tattataatt
    33001 tctttgaaga aaatgaaact aaatttgatg acatatttga taaattggtt aaacttagag
    33061 ataagatgtc taaaaaatta ggttttgaag attttgtaga gcttgggtat gttagaatga
    33121 tgagaagtga ctacagagaa tatatgataa aaaatgttag aaaacaagta ctaaaatatg
    33181 ttgttccaat ggcaaatgag ctttatgaaa aacaagctaa aagaattggt ctagaatatt
    33241 taagttacat agatgaaggc gtggaatttt taacaggaaa tgcaagttta aagggagatt
    33301 caagatacat aattaaaaat ggtaaaagga tgtattctga actgtcaaaa gaaacaaatg
    33361 aattttttga ttttatgtta gaaaatgaac ttatggattt agaaacgaag aaaggaaaag
    33421 gagctggagg gtattgtact tatataccag attataagtc accattcatt ttttctaatt
    33481 ttaatcaaac tgctgatgat atagatgtat taacacatga ggcaggacat gcatttcagt
    33541 tgtatatgtc tagatggatt gatatgccag aaattaattt tccaacgctt gatagttgtg
    33601 aaatccattc aatgagtatg gaatttataa catggccgtg gatggattta ttttttaaag
    33661 aagatacaga taagtacaaa ttcactcact tatcatcttc aattaaattt ataccttatg
    33721 gagtaattgt tgatgaattt caacactata tttacaaaaa tcctaatgta gataagtcaa
    33781 aacgaaaaga aatctggaga tttttagaga aaaaatactt acctcataga aaatatggtg
    33841 ataattcttt tttagagaga ggttgttggt ggtttaaaca aggacatata tttaaaaatc
    33901 cgttttatta tatagattat gtattagctc aaatttgtgc gttacaattt tggaaaaaaa
    33961 tgattcaaga tagagatgca ggatggaaag attatataaa tatctgtaaa gttggaggaa
    34021 caaaatcatt tttagatatt gtgagtatgg gaaatttgta ttctccattt gatgatggct
    34081 gtattgagtc tattataggt gatgttaaat cttggtttga tgaaataaat gacagcaaat
    34141 tataaaaaat tatgagaatt ttattgtata aaagtgaaaa attaaaaaat atataaaaag
    34201 tgattatttg ttgtgaaaat agtcttgttt ttaactaaat tgataaaata aactaaaaaa
    34261 gatttgtact taaaaaaaaa agaaaataca agaaatatgc aatatcaaca tttggaaaaa
    34321 aaagtaaaaa tgtatacaaa atacatataa aaaaactatt gcaaatttta tagaatgttt
    34381 atatacttaa gtcaacagag atagaaaaaa gctatttata gaaaacaaag gataaattgc
    34441 ttaattttaa attttctgaa aaaaataata atccggatga agagtatggg agactgcttt
    34501 ataaaaagcg accgaaggga caagtttaat cttacttacc caaaagatta gatgaaattc
    34561 tcaggtaaag gtgactgtac ttggacgggg ctctgaaaat gattttaaca gggaagacaa
    34621 attgggagat ttgtttttcc tgttttttat ttatcaagta ataaataaaa gtttgaaaga
    34681 gagtaaaaaa atgataataa ttacgaataa tccaaaagta aaagaagaag ttcaaggcag
    34741 agaagttttg tttaaagaca caacttatat tggaatctta gaagcaagta gagatttgat
    34801 acatgaaggt tatgaacttt tatctcatcc actctatggg agtgtaaagc caaatgaaac
    34861 accttacagg actgttatac taaagaaagg aaatcgtttg gatataaact ctttaacctt
    34921 aattgaagaa gcaatcatca ctgcaagtaa atttcagaat aataaaaaga ctccaaaatg
    34981 gacagaaagt gttcaagatg attttagagt aattgattat gatatatttt ataatacaat
    35041 acaaagaatg cagtatgaat aaaaaaaaat tgttaaaaag ttattaaaac agcctaaata
    35101 ttaaatctaa tttaatatta aataaaatga gaaatttaat tggggaggat aagtcaaaat
    35161 ggaaaatgtg tatgacttag taataattgg ttcaggacca gcagggcttg cagcaggtct
    35221 ttatggagca agagcaaagt taaaaactct aatcctagaa aaagacaaga ctggtggaca
    35281 aattgtaata acacatgaaa ttgcaaatta tccagggtca gttccaaatg caacaggacc
    35341 aagtttaata gctagaatgg ttgaacagtg taaagagttt ggagcagaaa tgctaagaga
    35401 caacattgtt gatactgagc tagatggaga tataaaagtt ttaaaagggg aaaaggcaga
    35461 atatagagca aaagcagtaa taataggaac aggagcaaca ccaagaaaga ttggttgtcc
    35521 tggagaaaaa gaacttactg gtaaaggggt ttcatattgt gcaacttgtg atgcagattt
    35581 ctttgaagat tttgaagtat ttgttgtagg tggaggagac agtgcattag aagaagcaat
    35641 gtatttaact aaatttgcta gaaaagtaac aattgtacat agaagacaag gatttagatg
    35701 tgctaagagt gttgaagaaa aagcaaaagc taatccaaag atagagtttt tattagatac
    35761 agtaattgaa gaaatcaaag gtgatggaat attagaatca gttgtattta aaaataaagt
    35821 tactggagaa actcatgaat actttgcaga tgaagaagat ggaacaatgg gagtatttgt
    35881 gttcgttgga ttagatgctc aaactgattt attcaaaggt aaagttgata tggatgaaaa
    35941 aggttacata ataactgatg aagatatgag aactaacata ccaggcgtat ttgcagcagg
    36001 agattgtaga tctaagactc taagacaggt tgtaactgca actaatgatg gagcaattgc
    36061 atctatagta gctgagaaat acattgatga aaaatttggg aactagaatc tgtaaattaa
    36121 gaaatagtgt ctgtaaattt aatgaataat atttaaaatt aaaattaata tttaaaatta
    36181 aaataaaaat ttcaaggggg aataagaaaa tgttagattt agataaggct acatttgaag
    36241 aagaagtttt aaatgcagaa ggttttgtat ttgttgattt ttggagtgaa ggttgtgagc
    36301 cttgtaaagc tttaatgcca gatgttcata aattagctga gacttatggg gataaaataa
    36361 aattctgtaa gatggataca acaaaagcta gaagattagc tataaaacaa aaagtattag
    36421 gtcttccaac tatggctata tacaaagatg gagaaaaagt agacgaagtt actaaggatg
    36481 atgctacagt tcctaatata gaaaacatga ttaaaaaata tctttaatat gttgataaat
    36541 tattaacatt aaaagaaaaa atctattttt atctgaagat aagaagaaaa aggtttttat
    36601 gtccaacttt gttaaaaaaa taattttaaa taaaataatt tttttacaat aaatattttt
    36661 ta
//
LOCUS       DAMJWU010000031        36130 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMJWU010000031 DAMJWU010000000
VERSION     DAMJWU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.031"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            32..1144
                     /locus_tag="PC460_001691"
     CDS             32..1144
                     /locus_tag="PC460_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF2600578.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATIITFLCIATFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="PC460_001692"
     CDS             1398..3422
                     /locus_tag="PC460_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2600579.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="PC460_001693"
     CDS             3642..4328
                     /locus_tag="PC460_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF2600580.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="PC460_001694"
     CDS             4423..5478
                     /locus_tag="PC460_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF2600581.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="PC460_001695"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="PC460_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2600582.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="PC460_001696"
     CDS             7195..8901
                     /locus_tag="PC460_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2600583.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="PC460_001697"
     CDS             8923..9996
                     /locus_tag="PC460_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF2600584.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="PC460_001698"
     CDS             10008..10730
                     /locus_tag="PC460_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2600585.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="PC460_001699"
     CDS             10746..11903
                     /locus_tag="PC460_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF2600586.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="PC460_001700"
     CDS             11906..12745
                     /locus_tag="PC460_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2600587.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="PC460_001701"
     CDS             12760..13935
                     /locus_tag="PC460_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF2600588.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="PC460_001702"
     CDS             13964..14713
                     /locus_tag="PC460_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2600589.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="PC460_001703"
     CDS             14735..15643
                     /locus_tag="PC460_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2600590.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="PC460_001704"
     CDS             15691..16443
                     /locus_tag="PC460_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2600591.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16421..17764
                     /locus_tag="PC460_001705"
     CDS             16421..17764
                     /locus_tag="PC460_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF2600592.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKVVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVK
                     VELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            17770..18861
                     /locus_tag="PC460_001706"
     CDS             17770..18861
                     /locus_tag="PC460_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2600593.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="PC460_001707"
     CDS             18945..20858
                     /locus_tag="PC460_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2600594.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKHN
                     VDEIIFSIANIEKRRKKEIIDVCKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="PC460_001708"
     CDS             21057..21764
                     /locus_tag="PC460_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2600595.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22066..24177
                     /locus_tag="PC460_001709"
     CDS             22066..24177
                     /locus_tag="PC460_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF2600596.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24375..25340)
                     /locus_tag="PC460_001710"
     CDS             complement(24375..25340)
                     /locus_tag="PC460_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2600597.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWSKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25625..26581)
                     /locus_tag="PC460_001711"
     CDS             complement(25625..26581)
                     /locus_tag="PC460_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2600598.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GHGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26949..27737
                     /locus_tag="PC460_001712"
     CDS             26949..27737
                     /locus_tag="PC460_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2600599.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27860..28330)
                     /locus_tag="PC460_001713"
     CDS             complement(27860..28330)
                     /locus_tag="PC460_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF2600600.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28469..29113
                     /gene="uppS"
                     /locus_tag="PC460_001714"
     CDS             28469..29113
                     /gene="uppS"
                     /locus_tag="PC460_001714"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF2600601.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29156..30061
                     /locus_tag="PC460_001715"
     CDS             29156..30061
                     /locus_tag="PC460_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2600602.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30376..31923
                     /locus_tag="PC460_001716"
     CDS             30376..31923
                     /locus_tag="PC460_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2600603.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            31926..32324
                     /locus_tag="PC460_001717"
     CDS             31926..32324
                     /locus_tag="PC460_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2600604.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32364..34097
                     /locus_tag="PC460_001718"
     CDS             32364..34097
                     /locus_tag="PC460_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2600605.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            34329..35540
                     /locus_tag="PC460_001719"
     CDS             34329..35540
                     /locus_tag="PC460_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2600606.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            35733..35993
                     /locus_tag="PC460_001720"
     CDS             35733..35993
                     /locus_tag="PC460_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF2600607.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT        14462 a         3785 c         6365 g        11518 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagcat atttactgct
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tagctacatt
      481 tataatagtt gggtactaca tcttagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acctaggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cccattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaat gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aaaacttatg
     2701 tttctgcata ccataataca aatttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaatagaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaaaaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agagtttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag tagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattggtct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaatgt atggatactg ccagcaggag cattattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcgttt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagagtta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gaccgattat tggcaaccac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagcac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttagaatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacac caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atcaaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagatttta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaagc aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ctattaaaag tatagatttt gaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaaaca agttttaaga
     7861 caaaatataa tagatgaata cgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gttttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagcaatga tagaagtttt
     8401 ataatgggat atgaggaaag ctatggttac ttaattggaa ctcatgctag agataaggat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa caatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaacg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcaacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctagtct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtatt atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgatccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcggca tgttttttaa
    11101 tggtagctat attattattt aatttatttc cttcattaat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaatacttt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccgg
    11461 taatacttac tttatttagt gtaattattg gtacagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggaatgtata cttggctgaa aaggggaact
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgaaaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agccgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagag tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggttca gccaacctag attatataaa ggtagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagaacttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa gccagacact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aagcataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagtgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttgtgg caactttagc ttatttaatt ccgatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggcgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taagaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agcacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag agattattga tgtatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcctaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgtaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt aaagacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 gcttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gtcatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtacttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttata tgaagaacta cttatgtcag
    20641 aagaaggtct gttggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtgccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggtg ttaatagcag
    21121 gagttgtcac agtagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tatagatggg gagcaactgg accaaatgct tttgattgtt
    21481 ctgggttcac ttcttatgtc tataaaaatg gagcaggagt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttcaggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaag aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg ttttttttat aataaattat ttcatattat aatcaaatat caaatataaa
    21961 ttttaactat attattcaat atttgacaga taatatgaat tttagatata cttaataaga
    22021 agtatattgt agaaaaaata aatttaaaaa attaaggggg atgaaatgaa aaaaataagt
    22081 atattagtat tatcattgat aatgacactt actatgtgta gtgtaagttc atttgctgat
    22141 tcatcaaatg ataaagaaat gagagctgct tggatatcta cagtttataa tttagactgg
    22201 cctaagacta agaataatga agccaagcaa aaaaaggaat acacagattt attggacaaa
    22261 ttaaaaagtg taggaataaa tacagcagtg gttcaagtaa gaccaaaatc agatgcttta
    22321 tataaatcaa atataaaccc atggtctgaa tatcttaccg gtacacaagg taaagaccct
    22381 ggatatgacc cattaccatt tttaatagaa gaagcacata aaagaggaat ggaatttcat
    22441 gcatggttta atccatatag aataactatg gcagatgaga gcatagataa attgccagca
    22501 aaccatccag caaagaaaaa tccaagctgg gtagttaaac atggaaataa gtattactac
    22561 gacccaggtt taccagaagt tagaaagtac atagtggata gtatagcaga agtagttcaa
    22621 aattacgata tagatggagt tcattttgat gattacttct atccaggagt aagtttcaat
    22681 gatactgcaa cataccaaaa atatggaaaa ggacaaaata aggatgattg gagaagagaa
    22741 aatgtaaata ctctacttag agatgtaaaa gcgtctataa agagtataaa gcctaatgtt
    22801 gtatttggtg taagcccagc aggtatatgg agaaataaga gtagtgaccc aacaggttca
    22861 gatacatcag gtaatgaaag ttatgtaggt acatatgcag atacaagagc atggataaaa
    22921 caaggattga ttgactatgt agttccacaa ctttactggc caattggtct taaagcagca
    22981 gattattcta aattagtggc atggtgggct aatgaagtaa aaggaacaaa tgtagactta
    23041 tacataggac aaggtatata taagcaaggt caatcgagtt atggtggaca aaatatagca
    23101 aaagaaatag tacaacaagt aacattaaat agaaagtata gtgaaataaa aggtagtatg
    23161 tatttctcag ctaaagatat agcaaatagt acttctatac aaaaggattt aaagagttta
    23221 tattctagta gtgaagaacc agttactcca ccatctaatg ttaaagtgga gaaattaaga
    23281 ggcgacgaaa gatatgacac agcagtagca ataagtaaaa aaggttgggc tacaaattca
    23341 gatacagtag tattagtaaa tgggtactct atagttgatg gtataacatc aacaccactt
    23401 gctacatcaa atgatgctcc tatactattg gtaaataagg ataatatacc tacatccact
    23461 aagaatgagt taaaaagact taatccaagt aaagttattt taattggagg aaataactct
    23521 attggagata aagttgagag tgagataaaa gatacattgt caaatgtatc aataaataga
    23581 gttggtggtt cagatagata tagtacatct ttaatgatag caaaagaatt agtaaaaact
    23641 aatccagtag aaaaactata tataacaagt ggtacaggtg aagcagattc tctttctata
    23701 gcttcaaaag caggagaaga aaagcaacca atagtattag tatcaaaaga taatgtaagt
    23761 gatgaagttt acaattggat atcagattta aaagtaaaag atgcttattt tataggtggt
    23821 aatctaagta tatctgattc agtaataaat aagctggata aggtcattac aaatgatgta
    23881 tctaaaaata gaatagctgg agaaaataga gaagaaacaa atggaaaagt aatacaaaaa
    23941 ttctatccaa atgctgaata ttcatcaatg tttgtaagta aaagtaatca attggtagat
    24001 gcattaacat caggtccttt agcggctaaa ttaaaatcac cagtagttat gcttggaaat
    24061 agtgtaacat ctgctcagaa aactgcttta gagcctaaga aaactactct agtatacgaa
    24121 gctggagatg gtataaatca aaacacatta aacactttct taaacttagt taagtaataa
    24181 aaaagttaat ttaaaataca gagtatttta caaatgtaga ttaaaatgaa aattatcatg
    24241 ataaattaga aaataaaaaa gaactatatg cattttgtaa tcaagtgtac acagcaactt
    24301 gaaaataatt atgcgatata gttctttttt agtaattaat cttatacttt ttaataaact
    24361 ttatgttgaa ttttttattg tttaaactct atgtcattaa atataaaatc gtgtaatatg
    24421 tctaaagtat ctgggtcaaa tctaagaacc catccagctt ttccatatat accacctgta
    24481 ctatgaattt catcatcaat aggaaattca aattgcttaa tatttaagtc acccattttt
    24541 aaaacttgag ctcctaaaga aagtattgca ttaggcttca tgttagtttt aacatatgga
    24601 agaactgcat ctaataaacc tggatattta gtaacaggta aagtttccgt tttcttgatt
    24661 atagcttgta taatctgtct ctgtctgcca tctctagcaa aggctgtatc gttgtgtctg
    24721 atacgtgcaa aagatagtgc ctgatatcca tttagagtct gctttccaga gtttctaatg
    24781 tattgtatgc tacctttatc atcactttta ctccatttat aagtttcagg aataaattta
    24841 tttagttctc gtatttctcc tttttcaaca gtaacctcaa cgccaccaat agcatcaatt
    24901 atatacataa atgattcgaa attaacacaa gcataatttt gaatatcaat attaaaattc
    24961 tcttcaattg tttgtattaa taaatcagct tgtccataag cataagcatg agtaagtttt
    25021 tgtttaccat gaccagggat gtctacatag ctatcacgag ctaatgatgt aagtttgata
    25081 tcattgtgtt tgttatcaat agtaagtatc atcatagcat ctgaccttga agaatcttca
    25141 tttggtctag catctgttcc cataagtagt atgttaatta tcccatcttc atttttatgg
    25201 tcattgttat ccaataaatc actacttatg ctcgagtcat gaatagcaga taatttataa
    25261 taaaaatatc catagacact tatagggaat attactacca agaaagccag tagtataaca
    25321 aatttcttta attttgacaa aagtacccct tctttcttct ttttcctgta tattttttaa
    25381 tagcatacac ttaattatat taaagtattg agatatattc aatacttgtt tcagttacaa
    25441 aaaaaagaca atgatataat ttataagaaa atttatcgac ctatatatat agttaaaagt
    25501 atatataata tattttttaa aagtaattta tattttaaaa taaaaacaca ccccataaat
    25561 acaaagtaat ttgcatttaa gaggtatgta tttatcatca aattattggt ttgttttgga
    25621 atttctaatc ttcaaccata atatctttaa atatgaaatc atgcattgca tctagttcat
    25681 attcctcaaa aggtattaca tagcctgcat tacctatttt tctaccattt tcagtagaaa
    25741 gaggaaactc catagatttc aaatttaaat ttcctatact taaaagttct tttcctaaag
    25801 atattatttc attaggattc atattagttt ttacatacgg aagtattgtg ttaactagat
    25861 taggatattt agtgactggt aaatccttaa caccatttat taaactctgt ataacacttc
    25921 tttgtcttct atctctttca tctgtactat cattttttct tatacgagaa aatgcaagtg
    25981 tctgatatcc atttaatttt tgctcaccag aatttcttat atatttaaga gaacctttat
    26041 tgtcattttt attccattta taagtttcag gaataaattt atttaattct cttatttcac
    26101 tcttatcaac agtaacagta actccaccta aagcatcaac tatatccata aaagagaaaa
    26161 agtcaactac ggcgtagttt tgtatatcaa gttggaagtt ttcttctata gtttctatta
    26221 ataagttagc ttgtccatat acgtaggcat gtgttaattt ttgttctcca tgtcctggta
    26281 tattaacata agtatctcta tttaatgaag ttagcttaag acttttattt ttattatcaa
    26341 tagtaagtat catcatggca tcagaacgag aaacttcttc tcctggtctt gcatctgtac
    26401 ctaacaataa tatgtttgtg atattttctt cgtttttata gtcattacta ctaagcgcct
    26461 ctgtatttat actatcatct tgtatagttt ttaatttagt aaaaacatat ccatatatac
    26521 taacaggaaa tgctataact aaaagggcta gaaatataac gaactttttg agtcctgaca
    26581 ataatatccc ctactttcta aattttttaa ttacacatag ttaattatat taaaaatttg
    26641 ttacataagc aataaaaaac acaataaatt agcataagtt tgtcataata taattatttt
    26701 tatagcattt tatagaagaa agtgtgtagt tattattgat tttattggaa tataattgta
    26761 tgaagactta aatttttata aactaatgtg tatattgaaa aaaatatttt cctgaatgta
    26821 gttatagcat cttatgaaag gtgcaaattg taatttaaaa tttttagtgt ataataagaa
    26881 ttgaaaattc aaaccgataa aaattaataa aaaataatta taaattaata atttggggga
    26941 ggaaatgtat gataaaatta atagcaacag atttagatgg aactttgtta gatgaaaaga
    27001 gcgaaataaa tccagagttt tataaagtat ttaaaaaatt aagagagaga gggataatgt
    27061 tttctgctgc tagtggcagg cagtatcaaa atctgataaa aaaatttgaa gatataaaag
    27121 atgatatgat gtttatatca gaaaatggaa cactagtagt atataaagga aaagaaatat
    27181 tatcaaatcc tttaaataaa gaattagtaa atgaaatcat agagacaaca agaagtataa
    27241 aagggaaaaa aattgtaatg agtgggaaaa aatacgctta tatagaaagc aaagatgaag
    27301 cgtttataca agaggttagt acttattatg caaagtttaa aattgtagaa gatttgacga
    27361 aagtagaggg agacatattg aagatagctg tgtttgactt caaaggtgca gaacataata
    27421 ataatatcta ctttgaaaag ttttctgata gagctcaagt atgtatttct ggagtggagt
    27481 ggcttgattt aactgcaaaa ggtgcaaata aagggagtgc cataaaaaaa gtacaaaaaa
    27541 tgttagatat aaaatatgaa gaaactatgg tatttggtga ccagttaaat gatgtggaaa
    27601 tgatgaaaag tgcctatcat agttatgcca tggaaaatgc aaatgaacat ttgaaacaaa
    27661 ttgctagatt tagagcaaaa agaaatactg aaaatggcgt tgtagataag ataaaagagg
    27721 ttataaaaat tggatagtca tatgtataga tgaaaaagat tattttgaag aaatcaaaaa
    27781 aatatcaatg taattttgat aaataagttt atttatgcga aaattacatt gatatttaat
    27841 aaacatcaat aatagatatt tattttttta tgatattcag tattttgttt tctatttgag
    27901 ttgcattttc agcagaaatt aaataaacat acctacctgc cttctcaatt atagtattat
    27961 ttgctatttc agaattgatt ttttctccat gtccttctcc aaaaggtgct tgtatcatat
    28021 cttttttata attttctaga gttttataga ttttatctac ctcgtcagtt tttataacga
    28081 taatatcttc tagagataat ttttcagaag aattaattac aaaaccttga attatgtctt
    28141 cctctacatc attaaaatag ttaaaatcct ttgcatcaaa gatttttgat tcctttataa
    28201 gtaaatcaga agattttaat gcttttttta tttttgatgt agaaacgttt ttgtaatcac
    28261 cacttgaaga acaactaacc attcctaatg ataagattat cgaaaaaaac aatattaaaa
    28321 tttttttcat gataaacctc caagtttttt aaactctgtc ttaaattagt ttactacata
    28381 attatataaa aataaatgta tttaatgtta cagttttgat taataatgta atattttgat
    28441 aataatacct attgagaggt gattagaaat gagaatacca aaacatgtag gaattatacc
    28501 ggatggaaat agaaggtggg caagtcaaaa tggtatgaca aaagataaag gatatgaaag
    28561 tggaatagac cctggactag agtcttatag attatgtaga gatttaggct gtgaagaggt
    28621 gactttttat ggatttacaa ctgataatac aagacgtcca tcagtgcaga caaaagcttt
    28681 tacaaaagca tgtattgatg ctgcaaatat attaatagat gaaggtgctg ctttattagt
    28741 tgtaggaaat acaaaatcgc caatgtttcc agaagaatta ttaccatata ctaatagaat
    28801 taaagccaaa gaaggtcaaa taaaagcaaa tcttctggta aattatggtt ggtattggga
    28861 cttaaataat atatcaggag tagaaaatgt aaataaaaat aatatacagg attacataca
    28921 gtcatttgat gtatccagac tagacttgat aatcagatgg ggtggaagaa ggagattaag
    28981 tggtttcctt cctgtacaat caatttatgc agatttttat gttatagata attattggcc
    29041 agattttaag agtgacgata taaaggacgc atttagttgg tatgatacac aagatgtcac
    29101 tttaggtgga taattaaaaa tattctgatt taaggttgta gagtgtttta aattattgaa
    29161 aaaaaaactt cttgatggta aaataacact aataatatgt aaaagtgtaa aaatttatac
    29221 caagaagggt gaagaaatgt caaaaggcaa taataacaat aattcaagaa acaaatctaa
    29281 aaagaccagt catctgaata gaaaaaagcg aaaattaaat aagaaaaagt tagcagtgtt
    29341 gatttgcttt acagttttat ttttatttat tgcatttaag gctactcaag gtgtagtagc
    29401 tttggtaaaa agtatggata aaagtaataa aactagtcaa caacaaaatg ttaactcaga
    29461 acaatttgac cttggtaatg aagaggaaaa taaaaagaaa aaatatactg tatttataga
    29521 cccaggtcat ggaggcaatg ataaaggaac tgaaagcaag accagtaata gatatgaaaa
    29581 agacctaaat ctacaaattg caaaaaaatt agcaaataaa ttatctaaac aaaaggatat
    29641 tcaagtagta gtatccagaa ctgatgatac atatatatca ttaaaggata gagctatctt
    29701 agcaaataat agttctgctg atgtgcttgt ttctattcat ttaaatgcag agaaaaatgg
    29761 taatacagca acaggtatag aaacttggta taggaataaa gctacagatg gttcaaaaga
    29821 acttgcacag acagtacaaa gtactatagt atcctatgtt aaagttagag ataggggaat
    29881 agttgaaaat aactttgaag tacttagaga atcaaatatg cctgctatat taatagagtg
    29941 tggtttttta acaacacctt cagaggaaca aaaaataata aatgaaaaat atcaagacca
    30001 acttgcggaa ggaattgttc aaggtgtact ttcataccta gattctaaag ggaataagta
    30061 aattaacttt actttaatgt gaggataaat ttaaaagttt aactgtgtaa attgtattaa
    30121 ctttatttcg taatgttgga aatattgttt gaatttagaa actatgaaat tagaaattat
    30181 tttatctata tctaatttca tagttttttt tgcaaaaaat ttatagttat aaaattttaa
    30241 aaaataaatt tagtataatg taatgtagtt atttatatgt gattttaact tgcattgatt
    30301 tgctatcaat tgtgctttat aattagtata tataaagttt aactgtaaga taatttaaaa
    30361 aataggggaa tttatatgaa taaaaatagc atgacaagaa tttacagttt aaataaaata
    30421 atttttatta taattggaat tttaggaata gttttactca caagacatat tgaggtagat
    30481 attaaagttt taattttaat tttattaacc ttgtttagta taaatgtatt tatttcatat
    30541 cttaaaatta agttgtatga ggataaaatt aatttaggat tgataaataa ttcagataag
    30601 tttataaata ttactaaaga tgagaacttc ctaaaacatg ttcaaaatta tattatcagt
    30661 aatgaaaaag aaaactgtgt aatggcttgt tttgatttat gtagactcaa acttataaat
    30721 gatatatatg gttatgagct aggggatgaa gtgttaaata atattttaag taacttaaaa
    30781 aattattttg gaaaagaagc tatttatgga aagttaaaaa gtgatgtttt tgtattaata
    30841 ataaagcttg aaaataggga acagaagatt ccttatttag taaatcttat taagaataaa
    30901 attgtgatac taagtcagat tgacagtttt aaaatggata taaatattga ggcttctatt
    30961 ggaatttata aatttaacaa taatgaaatt gatataaaaa aagctataga taatgctgat
    31021 atggcaaggc ttaaatcaaa aggactaaag cacatagagt atgtagaatt tgataatgct
    31081 atggaagaag aaatacaatc aattatgaaa atagagagag atttattctt ggctattaaa
    31141 aataaagagt ttgtaattca ctatcaacct aaagttgatt caagtacagg taacattata
    31201 ggttcagagg cattgataag atggttgcac ccatcattag gcatggttgg accaaataag
    31261 tttataccta tagctgaaaa aaatggtctg ataaacaata ttggtagatg gctaattcaa
    31321 gaagttttta ttaccattaa caagtggatt tcagaaggta taaatgtctt accagtctca
    31381 ataaatcttt ctagagtaga actatataaa aatgatttag ttgatttttt taaactgatg
    31441 tttaatacat ataatattcc aaaagaactt atagaattag aaattacaga aacgacagca
    31501 ctaagagatg taaaatttat aagtgaaagg ctttatgaga ttaaatcttt aggtatgaat
    31561 atatcattag atgattttgg agtaggtaat tcaaatttta tcaaccttaa ggggatacca
    31621 ctagatatta taaaaattga taggtcactt atattggata tagttacaaa tacaaaaaca
    31681 aagtttatgg tgaaagcaat tgttaacctt tcacatgatt taaacgtaac tgttatatgt
    31741 gaaggggtag aggatatgca tcaggtcaaa gtcttaagtg aactgggatg taatttcata
    31801 caaggttatg tattttttaa acctttggat gagatgaatt ataaaaaact tcttactgac
    31861 ggaagttata taaatctgaa agatactctt ttagataaat gcatgacagt tgaggaggag
    31921 taaggatgat ttcagaagat gtagattatt caataagaat cataagagct atatttaata
    31981 atggagaaga taaaccaata gatgctaaaa tgatattaaa aagtgaagat ataccaatac
    32041 taacactata cagactgctt agaaagcttt gtgcaaacaa tattcttact tttaacaaag
    32101 aagatggcaa gacatacatg ttaaatgtgt cattggatac aataagtcta agaactattg
    32161 tagaagtaat tgatggaaaa atatatataa ataaatgtac gagatatgta tctaattgca
    32221 gtaattgctt aaaaggttgt aaagtaaatc gtgaattgct agaaatagaa aaagaaattt
    32281 ataaactttt atcgagaaaa tctataagac aaatactaga ataaaaaatt ttcagctatg
    32341 ataataattg gaggttttcg gttatggatg atatatctca agataatttt ctactgtcta
    32401 aagagtatga gaatagctta gatgtagata ctaaaaaagc aagtggaata tattatactc
    32461 ctaaaataat tgtagattat attgtgaaaa aaactttaaa aaatcacgat ataattaaaa
    32521 atccatatcc acgaatactt gatatttcct gtgggtgtgg aaattttctt ttagaagtat
    32581 atgatatttt atatgactta tttgaggaaa atatatatga attgaaaaag aagtatgatg
    32641 aaaattattg gactgttgat aacatacacc gtcatatatt aaactattgt atatatggtg
    32701 ctgatattga tgaaaaagct ataagtatat taaaagatag cttaacaaat aaaaaggtag
    32761 taaatgattt agatgaatct gatataaaga ttaatctatt ttgttgtgat agtcttaaaa
    32821 agaaatggcg atataagttt gattacatag tgggtaatcc accctatatt ggtcataaaa
    32881 aacttgaaaa gaaatataaa aaatttctcc tagaaaaata tagtgaagtt tataaggata
    32941 aagctgactt atatttttgt ttttataaaa agattataga tattttaaag caaggaggga
    33001 ttggcagtgt tataactcca agatattttt tagaaagtct atcaggtaaa gatttaagag
    33061 aatatattaa atcaaatgtc aatgttcaag aaatagtgga ttttttaggt gcaaatatat
    33121 ttaaaaatat aggggtatct agttgtattc taacttttga taagaaaaaa acaaaagaga
    33181 catatataga tgtatttaaa atcaaaaatg aggatatatg tataaataaa tttgaaacat
    33241 tggaagaact tctaaagagt agcaaatttg aacattttaa tataaatcag agattgctat
    33301 ctgacgaatg gatacttgta aataaagatg atgaaacttt ttataataag atacaagaaa
    33361 aatgtaaata tagcttggaa gatatagcaa taagttttca aggaataatt acagggtgtg
    33421 ataaagcgtt tattttaagt aaagatgatg taaagttgaa ccttgtagat gataaatttt
    33481 taaaatgttg gataaaaagt aagaatataa ataaatatat agtagataaa agtgaatata
    33541 ggctaatata ctcaaatgat atagataatg agaatacaaa taaaaggata ctagatgaaa
    33601 ttatagggct atataaaaca aaattagaaa acagaagaga atgtaaaagt ggaataagga
    33661 aatggtatga acttcaatgg ggtagagaaa agcttttctt tgaaagaaaa aagataatgt
    33721 atccttataa atcaaatgaa aatagatttg ctatagatta tgataataat ttttcaagtg
    33781 cagacgtata ttcttttttt ataaaagaag agtatttaga taaattttca tatgaatatc
    33841 tagttggaat tttaaattcc agtgtttatg ataaatactt taaaataact gctaaaaaaa
    33901 tgagtaaaaa tatatatgat tactatccta ataaggttat gaagataagg atttttaggg
    33961 ataataatta tgaagagata gagaatttat caaagcaaat aatatcaatt ttattaaata
    34021 agtctataga taaaggaaaa gtagaaaaat tgcagataaa aatggataac ttgataatgg
    34081 attctttagg tatataagca gttgtaagta ttaaaaaatt ataatctttt aattgtttat
    34141 ttaaatttag aaataaaaag ttagccatat aaaaaatatt aaaaatatat acttttcaat
    34201 atttttttga taaaataaga atgaatttgt gcaattttat ttttctccaa taactaaaaa
    34261 tagttttttg aattaatatt tgcttaagaa taaaaatcag aaacgttttt gatatagtag
    34321 gtgataatat gaaaggatta gttttagaag gtggaggaac aaaaggagcc taccaaatag
    34381 gtgcatataa agctttaaga gagcttggta tagagtttca aggagtaact ggcacatcaa
    34441 ttggtgcttt aaatggagca tatataattc aaaatgatat tgaagtaatg gaagatattt
    34501 ggttaaatta tgactatact cattttatga atattgatag agaaacctat gagaggttta
    34561 aaaatgtaga tttcactact aaaaatctca atgcagtgtt tgagcttata aataaagcta
    34621 gaaaaaatga aggtatagat ataagcccat taagacaatt aatggagaca acattaaatg
    34681 aagacataat aaggaaatca aaaaaagact ttggacttgt tacggtctgg tgggataaaa
    34741 aaattaatcc atatccattg tatatcgaag atatacctaa aggaagattg attgattatt
    34801 taattgctag ttccagtttg cctatttttc aaacgaacat gatggatgat aagttgtatc
    34861 ttgatggaat gttttgtgat aacatgccaa taggactctt ggaagacaaa ggttatgaag
    34921 atgttgttgt aattaggttg attgatgatt ttattgggaa aatgaatcta aatcgacatc
    34981 aaaatataaa tataaaaact ataataccat cagaatatct aggaggttct cttaataaag
    35041 atagagataa tgtagaaaaa aacatccgac taggttactt agatactatg aaagcatatg
    35101 aaagatgcac tggaattaag tattattttg atgtagagta taaatataat gaagaatatt
    35161 gttttgataa atttagaagt atgagcaatg aaacaataca atatatctgt aatttattga
    35221 atataaaaag agatgcttca cttagaactg ttatggaaag tgtaatacca aagcttggtg
    35281 aggtgcttgg attgccaaag gatttttcat ataaagattt gttttactgt atatatgaaa
    35341 agaaacttga agaaaataat ataaacagga taaaattata tgattttaac aaggttatag
    35401 atgttgtaaa tacaaatatt gattctaata gaaatttagc ttctaatttt gaagttagac
    35461 ctaaaattag tattaaaaag aacaaaaaca caaaattatt gacaaattgt attataaaag
    35521 acataaaaaa tcaaagttaa ataaaaaaca aaaaaaaatt tgtaattata ctcgaaaaaa
    35581 gtgtatatta gtagtataat atttttgtag cactagtggg aacaaaaaat gaccatagga
    35641 caaaaaagta ccacttagta ccattaaagt aaaaaacagg aaaacaattt taaaaaatat
    35701 aaaatgtaaa gtttatagga ggatttaaaa aaatggaaaa agtagttagt atcaaaaatg
    35761 caagtggatt acatgctaga ccagcaggaa tgtttgttaa gaaggcagct gaatttaaat
    35821 caacagtaga ggttgtagca aaagacaaaa ctgtaaatgc aaaatcaata atgggaataa
    35881 tgagtttagg tttagctcaa ggagaagaat taaagttagt tgtaaatgga gaagatgaag
    35941 aagtagcttt agctgcaatg gttgaattag ttgaaagtgg atttggagaa tagatataat
    36001 aattttttaa aaatttgata tttagaatct aatattactt aaagtaaagt aacagagaag
    36061 tattaaacat atacagacca acatttgtag atatttaatt tctttgttac tttcatgttt
    36121 atttaatcag
//
LOCUS       DAMJWU010000032        34830 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMJWU010000032 DAMJWU010000000
VERSION     DAMJWU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.032"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            58..795
                     /gene="cas6"
                     /locus_tag="PC460_001721"
     CDS             58..795
                     /gene="cas6"
                     /locus_tag="PC460_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2600608.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            800..2275
                     /locus_tag="PC460_001722"
     CDS             800..2275
                     /locus_tag="PC460_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600609.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFCELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMEKNVKRIVDIVIKSN
                     IEIDEIDKLDLKQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRIDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     MNTRNLGKKMINNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGREEYKMKNYIESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2295..3311
                     /gene="cas7i"
                     /locus_tag="PC460_001723"
     CDS             2295..3311
                     /gene="cas7i"
                     /locus_tag="PC460_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2600610.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYRDKVHA
                     KTSIATITYDLRRILHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKEKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEREENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEEDGKLLLPEERKFMSKELREK
                     AVKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGV
                     IETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMW
                     YLKE"
     gene            3327..4124
                     /gene="cas5"
                     /locus_tag="PC460_001724"
     CDS             3327..4124
                     /gene="cas5"
                     /locus_tag="PC460_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2600611.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4151..6559
                     /locus_tag="PC460_001725"
     CDS             4151..6559
                     /locus_tag="PC460_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2600612.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7001..7948
                     /gene="prmA"
                     /locus_tag="PC460_001726"
     CDS             7001..7948
                     /gene="prmA"
                     /locus_tag="PC460_001726"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF2600613.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            7967..8725
                     /locus_tag="PC460_001727"
     CDS             7967..8725
                     /locus_tag="PC460_001727"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF2600614.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8727..10025
                     /gene="mtaB"
                     /locus_tag="PC460_001728"
     CDS             8727..10025
                     /gene="mtaB"
                     /locus_tag="PC460_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF2600615.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10068..10418
                     /locus_tag="PC460_001729"
     CDS             10068..10418
                     /locus_tag="PC460_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF2600616.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10581..10760
                     /locus_tag="PC460_001730"
     CDS             10581..10760
                     /locus_tag="PC460_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF2600617.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10790..11233
                     /locus_tag="PC460_001731"
     CDS             10790..11233
                     /locus_tag="PC460_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF2600618.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11344..11841
                     /locus_tag="PC460_001732"
     CDS             11344..11841
                     /locus_tag="PC460_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF2600619.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12034..12597
                     /gene="raiA"
                     /locus_tag="PC460_001733"
     CDS             12034..12597
                     /gene="raiA"
                     /locus_tag="PC460_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2600620.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12810..13046
                     /locus_tag="PC460_001734"
     CDS             12810..13046
                     /locus_tag="PC460_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF2600621.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13059..14402
                     /gene="yqfD"
                     /locus_tag="PC460_001735"
     CDS             13059..14402
                     /gene="yqfD"
                     /locus_tag="PC460_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF2600622.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14341..15456
                     /locus_tag="PC460_001736"
     CDS             14341..15456
                     /locus_tag="PC460_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF2600623.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15513..15974
                     /gene="ybeY"
                     /locus_tag="PC460_001737"
     CDS             15513..15974
                     /gene="ybeY"
                     /locus_tag="PC460_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF2600624.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            15980..16699
                     /locus_tag="PC460_001738"
     CDS             15980..16699
                     /locus_tag="PC460_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF2600625.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16818..17219
                     /locus_tag="PC460_001739"
     CDS             16818..17219
                     /locus_tag="PC460_001739"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2600626.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17283..18176
                     /gene="era"
                     /locus_tag="PC460_001740"
     CDS             17283..18176
                     /gene="era"
                     /locus_tag="PC460_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF2600627.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18188..19567
                     /gene="mgtE"
                     /locus_tag="PC460_001741"
     CDS             18188..19567
                     /gene="mgtE"
                     /locus_tag="PC460_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF2600628.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19640..19795
                     /locus_tag="PC460_001742"
     CDS             19640..19795
                     /locus_tag="PC460_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF2600629.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19761..20534
                     /gene="recO"
                     /locus_tag="PC460_001743"
     CDS             19761..20534
                     /gene="recO"
                     /locus_tag="PC460_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF2600630.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20527..21126
                     /locus_tag="PC460_001744"
     CDS             20527..21126
                     /locus_tag="PC460_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF2600631.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21286..22164
                     /gene="glyQ"
                     /locus_tag="PC460_001745"
     CDS             21286..22164
                     /gene="glyQ"
                     /locus_tag="PC460_001745"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF2600632.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22164..24230
                     /locus_tag="PC460_001746"
     CDS             22164..24230
                     /locus_tag="PC460_001746"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF2600633.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKILIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(24396..25943)
                     /locus_tag="PC460_001747"
     CDS             complement(24396..25943)
                     /locus_tag="PC460_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF2600634.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            26223..26990
                     /locus_tag="PC460_001748"
     CDS             26223..26990
                     /locus_tag="PC460_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2600635.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27273..27470
                     /locus_tag="PC460_001749"
     CDS             27273..27470
                     /locus_tag="PC460_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2600636.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27499..28575
                     /gene="vorB"
                     /locus_tag="PC460_001750"
     CDS             27499..28575
                     /gene="vorB"
                     /locus_tag="PC460_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2600637.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28578..29297
                     /locus_tag="PC460_001751"
     CDS             28578..29297
                     /locus_tag="PC460_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2600638.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29310..29849
                     /locus_tag="PC460_001752"
     CDS             29310..29849
                     /locus_tag="PC460_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2600639.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            30006..31064
                     /gene="buk"
                     /locus_tag="PC460_001753"
     CDS             30006..31064
                     /gene="buk"
                     /locus_tag="PC460_001753"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2600640.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31082..31975
                     /locus_tag="PC460_001754"
     CDS             31082..31975
                     /locus_tag="PC460_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2600641.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            32005..33213
                     /locus_tag="PC460_001755"
     CDS             32005..33213
                     /locus_tag="PC460_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2600642.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33237..34496
                     /locus_tag="PC460_001756"
     CDS             33237..34496
                     /locus_tag="PC460_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2600643.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        14063 a         3534 c         6445 g        10788 t
ORIGIN      
        1 ttttttatat tacaaataga gaataatgaa ttatactaga aagtaggagg tgaaaatatg
       61 agcagaattt caatttttat ggaatcatgt aatggcaaat ttccaaatga aaattcaatg
      121 ttgtcagttt cctttataaa aaatatttta aatgcagaaa acaaagattt tgctaaaaac
      181 atatttaact atggggaaga aagaaaaaac aataagcaaa taaaggatat aaatacagcc
      241 atttacatac caaatcttat aagaagcaaa aatgagttac ttgttgatgg agatataata
      301 ttcaatataa gtttttataa ttattctatg ttcagtaaat tatacaatgg aattttaaaa
      361 gtaaaggagc tagaatataa aggttataga tttaaaataa aaaatataaa aatacataaa
      421 gaacacgaga taaaagaaaa tggtgttata tttaaaacta tgtctcctat aatagtaaag
      481 aataaagaag gtaaatattt agatgtagaa gattcaaatt atatagaatg tttaaactat
      541 atagctaatc taactctgga gagtataaga ggtagtgggc ttagaaagcc attggaattt
      601 atacctctta acttcaaaaa aagagtgtta aaagaaaaga ttcgtggatt taaagagaga
      661 gaattttact atataaatgc ttatgcagga acattttttt taaaaggaaa tatggaagat
      721 ttaaatgcac tgtataaaat gggaattgga tatagaagga cggaaaatgc aggaatggtt
      781 gatatattaa agtagcgagg tgagagaaat aaaagctaaa ttttatagat caggtgatat
      841 tgtaagagat attggcattg tttacttttg tgaattacta atggatttaa aaaacgaact
      901 agaaaaaaat gattataagc taaaatttac ttgtgagttg aatagaaatt atttatcttt
      961 agaatctgca gaatatatag accctaagta tatatctgat tatatattag aaaatcaact
     1021 ttttaaggta tttaaaaatg ggaaaaagga aaccttagaa aaaatattta gtaatataga
     1081 taatttaaca tatgtaaatt ttattgaaga attggataaa tcttctgcaa ctgagaaaca
     1141 aaaggaaggg attaaaaaag attttaaaaa cagaagattt ccatatgtta gaaatagtgg
     1201 taagtatgga ttaaacacga gtctagaaaa tatggaaaaa aatgtaaaaa gaatagtcga
     1261 tattgtgatt aaatcaaata tagagataga tgaaatagat aaacttgatt tgaaacaata
     1321 tgaagaaaaa gatagtgtat gtaatgtttg caatataaat aaaacaacaa aactagatat
     1381 tgatttaaga gaacgaaagg actctaagta taacttttta tttagaggtg cagaaaaatc
     1441 aggttttaaa agaagtggac aagttgaaag taatatttgt tttgagtgtg aatttttcaa
     1501 cttaatgtgt cttttatata taaatttaaa aagaccaatg gtttttgcat atacgaatga
     1561 tttaagagaa ttagcatttt taaatcataa gataatgctt aaaagaaaaa tgtattcaga
     1621 taaatcattt tataaaaaac ttttgcatga aaaaataagt tcaattagac tatataggat
     1681 tgatattgat acgaataaag gtataattct aaaatttgac tctattatag agtataaaga
     1741 attattaaaa aaaattgaac ttatagatat tatagataat tataattttt caagagagcc
     1801 aatgaatact agaaatttgg gtaaaaaaat gattaataat gggaatttat caaatttaaa
     1861 agaattatta cttgataata taagtgtttt aaggcaacta actggaactt ctcgagaaat
     1921 ggattttgta tctagttcat ataatatcgg tttatatata aaattgtgtt ggattgtaga
     1981 tggaagggag gagtataaaa tgaaaaacta tatagaaagt aatttaatat attcaagagt
     2041 aggtaaagac ttatttaata aaatgacaga tgaatccaga agaaaaaatt tttctatgaa
     2101 agtgattcaa ctacttaagt ctaatgatag aactcagcta tttcaaacta taatgcatgt
     2161 gttagtttct aatggaatct taataggaga agggtttgta gaaggtctaa tgcagagttc
     2221 tgaattagaa ttaaatacga atgtaggtct atttattcaa gaaatcatga aataaaaaag
     2281 ggggaaaatt taaaatgaat gaaaaaagag taaaaggagt aacattaact atactgacag
     2341 aaagcccagt tccactttcg tatgaccaag ggtatggaaa ttatacacca ataaaaaaag
     2401 aacaatatag agataaagta catgcaaaaa ctagtatagc tacaataaca tatgatttaa
     2461 gaagaatact ccatcaagaa tatggttgga atttaagtaa tattgttttt ggaaaaaaag
     2521 gaaatatata tccatctatt aagaaaaatg taggatgtgt agatgaaaat ggattagaga
     2581 ctgatgtatt tggttattta ataccactta aagagaaagg ttctataagt aaggcttctc
     2641 cgcttagaat aatacctttt aggtcactaa attcatatag aggttcaaca cagttgataa
     2701 caaatagagg ttttttaagt agcgaatttg gtagaaaata ttatgatgag agggaagaaa
     2761 atgaagtact tagagatgaa aactttccaa caacacaagc attagcaata gaagagactt
     2821 tatcagatta ctacgtttat acaataacat tggagcttga tagaatagga gtagtagaag
     2881 tagaagaaga tggaaagtta cttttaccag aagaaagaaa atttatgagt aaggagttaa
     2941 gagaaaaagc agtaaaagat atattagatg caattactgt gtttacaaga aacataaaac
     3001 attcatcagt actattaaaa ccacttgcag taatgggtgg agcttttgat aaagttgtac
     3061 catttttttg ggatgatgta gattataatg ctgatagtgg tgagataaat ttagagggag
     3121 tcatagaaac tatagaaagt tatagcctta aagaaagtaa cacaatactt gctatcaatg
     3181 acagattaaa aatttctaat aaaaaggaat taaataagtt taatttaagt aaataccctg
     3241 taaaagagat taaaaatcta gcagatagat tggaaattgg agaagataat atgtggtatt
     3301 taaaagagta ggaggttttt tgaattatga aagtattaca atgtaaactt aagcaaccaa
     3361 ctgctcacta tagagaccca aaagtatttc aaaatgaata tataagtact ttaaatttac
     3421 catctaagac cacaataatg ggaatgataa cgtatttatg tgatagaaga ttaaactctg
     3481 atatagatat aggaataatt ggaacacatc atcatagaga actagaattt tcaagaggag
     3541 agaacattga tttttggaat gaatatacaa atatgagaaa aggcaaagat aaagaaaagt
     3601 ttttgcttca aggaaactac tatgattact ataaagaaca taaagctcaa aatagcatat
     3661 taaactatga agttcttaaa gaagtagaac tgactatatt tatttcttgc aaggatgatg
     3721 aagaacttga atttattaga aaaaagcttg aaagtccatg taaatatgca aatttaggaa
     3781 gaaaagaaga ttttgtaata ccaagtgaaa aagggtgttt tgtgaaagaa gttgagttaa
     3841 aagaggttat acctatgaat actcgtgatg ctataaaaga aaatataaaa ttaaagaata
     3901 catatgtaag agttgactta agagataaag agaatgtcga gactataata aatcaaggtg
     3961 tattgattgc attaccttat aaatataaag atttagaagc aaatagagat gatagagttt
     4021 atgagttttg ccattatata tatgttgata atgatggtat atatcctaaa aatattaagg
     4081 taaatgtatg tattgataca aaagaagttt ttacatggct ttaaattatt ttgaaagata
     4141 ggtgtttatc atgaaaaata tcatgcaaaa agaaatatta gctaagtcat ataatttaag
     4201 aactaatcaa caagagcaaa cattagttga acatattaag gatttatttg aagtattaga
     4261 atctatatta gaactaaatc tatattcaga taaagatgta gagatactaa aaatatgttg
     4321 tgctttgcac gatttaggaa aaattaattc aattatgcaa caaaaaatgg agattaataa
     4381 taaaatatcg tatagttgct cagaagaaga gaggaaaaag ttagagtctg acaaaaaatc
     4441 tttaaaaaag atagcaagac ataatatatt ttctggagca tttttaaaag atatattaga
     4501 aaaaatgaac ttgtcagaag aagataagtt atatatatat aaaagtataa tgttacatca
     4561 tggaaactat gaagattata tgaggttaag tattagtaaa gttcaagaag aaatttatga
     4621 gtatattgaa aaaggcattt tagaaaaaga agagtttaat ctgaaagata tagaaagtta
     4681 tataaatagc attttaaatg tagactttaa atttggtgaa gatgtattgg attatgatta
     4741 catagataaa ttaagtgaaa gtatgtttat agacagtgat tataacaatg gtcaaataga
     4801 tgattcagtt ttaaattata gaaagtttaa gtatatttta tataaaggga tgctgaactt
     4861 aatagaccat agtgcatcaa gtagacagaa aggtataaaa ttttataacg attttactga
     4921 tgaagaaatt gataatatga ttttaaatga gatatataag agtcagggta atttggaaaa
     4981 aaataatata gagttcaata ctatacaaaa aaggttgaga gaactttctg gaagaaatgt
     5041 tctgactgtt gcatttacag gttcaggaaa aacagctgca gattatagaa agacttttaa
     5101 aagaaaattc tttttagtgc caaataaaat atcagcagaa agtttttata gaaaaaatat
     5161 ttttcaaaat aaaaatgatt tagatacaag aagtagtaat gattacattg gattacttca
     5221 tggagatata aacttatatt cagagaatga agataatgga gaacatgatt ttgtattgac
     5281 attaagggat atagatttga gtataaactt ttgtaaacct tgtgtaattg caacagttga
     5341 ccagttatta cttagtatgt ttaaatttcc gggatatgaa aaaatattcg cagccgtaaa
     5401 aaatgcatct ataactgttg atgaagtaca tttattggaa ccaaagatgt ttttgatttt
     5461 gatatatttt atgcagtttg catgcaagta cttagatgtt gattttcact taatgacagc
     5521 tacacttcca aaatcatata aagagcaaat gataaacaag ggaattattt ttcaagaaga
     5581 aagtaatgaa aatgttactg ataaaggaga aatagttttt attgagagca acaaggaaga
     5641 agagatttgt gaaggtaaag atgtaaaggt ttcttttata aaggaaaaag aaatcaagtc
     5701 aatagttgaa gatgctttag aaaataaaca aaaaattctt ataataaaga atactattga
     5761 ttcagtcaat agaacatatg agtttttaaa agaaaattta tctgataagt atggtggtgt
     5821 agatatagat gttttacata gtagatttaa gttcaaagat aaaaaagaga aatatagtaa
     5881 aatattaaat ggagagggtg atatttggat atcaactcaa tcagtagaaa tatccttgga
     5941 tttggatttc aatataataa taagtgattt agcaactatg gatagcttga tacaaagaat
     6001 gggtagatgt aatagaaata ataaatatga atatggaaac ttttatattc tacctagtga
     6061 agataaaatt tatgataaca aacttaagaa cacaactaaa aacatcttaa aggacatatt
     6121 aaaaactaac tctattttta caatgggaat tagaaaaact attttggatg attactatga
     6181 taatagtgta gtcaaaaaat attttgaaga gaattttata agttgtgata aagaaattaa
     6241 gaatatttat ggtataaata aagaaatttt tgatggattg gatttaattt ttaattttga
     6301 accttacaaa aatattgttg atagtaagaa ggaagcagct aaaattttta gagatgttga
     6361 tgttagttac aaaattattc tggaagaaga tttttataag gaagatagag agttacaaca
     6421 agattcgatt caagtatcag ggtttatatt taatagatta tattattatg ggttaataag
     6481 caaagtagaa ggatacatgg tactaaaatc tagtagtaag tttgagtata atagtactgt
     6541 aggcttaatt ttgaagtgag catatgttta aatataatat acctttagac attgaaaata
     6601 caaaaagtac tactgtaagc tcactgtaaa aactattttt ttaaaaggaa aaataagttg
     6661 tgagatagag tgtttacaag tgtatataga taataaattt gaggtttaag attaactaag
     6721 tgggtttcag gtacatcgcg aaaataatta taagttggag attttgtatg tttaagatta
     6781 actaagtgga tttcaaatat tgatatagta gatataatag aagttttatt attaggttta
     6841 aaattaacta atcggatttt taaatattct gactattata taaggatatt attagcttga
     6901 atttaaaacc aataatataa aacaacaaaa tattatattt atgatataat aaaagttatg
     6961 gaaaaacagg aatattacta tagagaagag aggtaaactt atgaataatt ggatagaagt
     7021 aactataaaa acaacaacag aagcagttga acctataacg aatatactat atgaacaagg
     7081 agctggagga gctgtaatag aagacccaaa agacttttta ttccaaaaga aaaatgaact
     7141 agactgggat tatgttgaag aagaagtatt taaaaagaat gaagaagatg atgttcttat
     7201 aaaaacttat gtttctgaag aaaaaaatgt gatggaattt gtcgaaataa taaagcaaaa
     7261 agttttaggg ttaaaagatt ttggtataga tataggtgaa ggaagtgtat cattggacca
     7321 agtaaatgaa gctgattggg caaatgcatg gaaagcatac tacaaaccaa caaaagtagg
     7381 acaaagggta gtagtaaagc ctacatggga agattatgct atgcaagagg gggatttaat
     7441 catagagctt gaccctggaa tggcatttgg tacaggtaca catgagacta ctagtatgtg
     7501 cattagagaa ttagaaaagt acgtgaataa agattctaaa gtatttgata ttggctgtgg
     7561 tagtggaatc cttgcaatag cagcagctaa attaggagca aaagaagttg tagctgtaga
     7621 tttagatgaa gtggctgtaa aagttgcaaa agaaaatgtt cttgaaaata aagttgaaaa
     7681 aagtgtatct gtaatgcatg gtaatttaac tgatgtaata aaagataaag cggatgtaat
     7741 agttgcaaat ataatagctg atataataaa gatacttgca aaagatgttc aaaactttat
     7801 gaaagaagat gctatattca tatcttcagg tataattttg gataaagtag aagaagttaa
     7861 agaaagctta attgaaaatg gatttgaaat agttgaagtt caaaaattag gtgaatggtc
     7921 agcaatagtt tctaagctta aaaaataagg gatatggtga cgtataatgg atagattttt
     7981 tgttgaaaaa aataatataa atctacaaga taaaacttgc actatagaag gtgaagatgt
     8041 aaaacatatt tcaaaagttt taagatgcaa attaggtgag aaacttgaaa tatgtgacaa
     8101 gaataataat gagtatattt gtgagataat gaatatagat aagagtatag ttaatttaga
     8161 aattttagaa aaagttgata taaatagaga atcagaactt aaagtaaggc tataccaggg
     8221 acttccaaaa gctccaaaaa tggaaatgat acttcaaaaa ttaactgaag tgggtgtaga
     8281 agaaataata ttagttcaaa ctaagagaag tgttgttaaa gttgatgaca aaaaagaaga
     8341 taaaaaattt gaacgttggg aaagaataat ttatgaagca gcaaagcaaa gcaaaagagg
     8401 taagatacca aaattaagag gtgttttaag ttttaaagaa gctttagaag atatgaaaaa
     8461 aaataatgta aatatttgcc cttatgaaaa tgaaagaaca gtttcaataa aacatgcact
     8521 aaaaaaatgt gattctaata tagatagtgt tggaattttt ataggtccag aaggtggatt
     8581 ttctgaagaa gaaatagaac aaatacaaaa aaataattgt aatgttgtat cattggggcc
     8641 tagaatatta agaactgaga cagcttctgt tgtagcatca acaatagctt tgtatgaact
     8701 aagtgattta ggaggagaaa aataaattga aaaaagtagc tttttataca cttgggtgta
     8761 aagttaatca atatgaaaca gaagctatgc tagaactatt tgagaaagat ggttatgaac
     8821 aagtcaatag tgaagagtat gctgatgttt atgttataaa tacatgtaca gttactcata
     8881 tgagtgatag aaaatctcgt caatatataa gacgagttaa aaagaagaat ccagatgcaa
     8941 taatagccgt tgtaggatgt tattctcagg tatctccaga agaaattttg gatatagaag
     9001 aagtaaattt agtcatgggc acaaatgata gaagaaagat tgttgaagaa attaaaaaga
     9061 taaattcaag taagaaagtt agtacagttg atgacataat gaaagttaaa gcttttgaag
     9121 aaatagaaat cagccaaact aatggaaaga ctagagcatt tatgaaaata caagatggat
     9181 gtgacagatt ttgtacttat tgtatcattc cttatgcccg aggacgcgtt agaagtagag
     9241 atattgatag tatagtcgat gaagtgaaaa aattggctaa taatggctat aaagaggttg
     9301 tattaacagg aatacatgtt gcctcttatg gaaaagattt aaaggatagg gatattaaac
     9361 ttcttgatgt aataaaacaa ataaaccaga tagaaaaaat agaaagaatt aggcttagtt
     9421 ctgttgaacc aattttattt acagatgaat ttgtaaatga agttttaaag atggataaag
     9481 tttgtcctca ttaccattta tcacttcaaa gtggatgtga tgaaactcta aaaagaatga
     9541 atagacgata cacaacttta gagtacaaaa ctattgtaga tagattgaga agtaaaatgc
     9601 cagatgtggc aataactaca gatgtaatag taggttttcc tggagagaca aatgaagagt
     9661 ttaaaaaaac gtacgagttt ttaaaagaaa tagagctttc acaaatgcat atttttaaat
     9721 attcaccaag aaaaggaact ccagctgcaa ctatggaaaa tcaagttgac cctcaaatga
     9781 aacactttag aagtgaacaa cttttaaatt taagtaaagt aaattttaat aaatttgcaa
     9841 ctaaatttat cggccgtgag ttggatgttt tatttgaaca aaatatagaa ggcaataaat
     9901 atgagggatt aacttcaaat tatataagag ttgttgttga aagtgacaaa aatatacaag
     9961 gtcagatatt aaaagtgaaa ataaatgatg taaaagatga atatgtagaa ggaattttgc
    10021 tataacatgt agaaattata atatttataa gataggaggt ggaaagaatg gattgtattt
    10081 tttgtaaaat agcgaacgga gaaatacctt caactaaagt ttatgaagat gatagagtct
    10141 tagcttttaa tgacttgaac ccagttgctc catatcatat attagttgta cctaaaaaac
    10201 attatgatag tttaattgat attcctgata aagagatgga cattgtgtca catattcatg
    10261 ttgttattaa taaaatagct aaagagaaag gttttgacca aactggattc agagtcataa
    10321 ataattgtgg aagtgatgga gggcaagaag ttaaacattt gcactatcat attttagctg
    10381 gtaaaaagct tcctaattac gaagcgggac aaaactaaac taaattattg aaaaattgag
    10441 ttccttcttg ataaaaatac ttgataaaag taaaaattta gtttataata aacaaagtgt
    10501 taggttgtaa tgcatattat gcaaaaagaa catagttaca gtcccagcat aattgcgcta
    10561 ttggagggag ggaaaaagaa atgtcagaag taagagttag agagaatgaa acattagaca
    10621 gcgctttaag aagattcaag cgtcaatgtg caatgtctgg catcatgtca gaagtgagaa
    10681 aaagagagca ctatgataag ccaagcgtta agcgtaagaa aaaagctgaa gcagcaagaa
    10741 gaaaaaacgc taagaaatag tagtaaataa gaataaagag gtgaagggga tgtcccttaa
    10801 gcaaaagtta caagaggatt tgaaatcttc gatgaaaaac aaagatacag tgagaaaatc
    10861 tgtagtaact ttaataagag cttctataaa gcagtatgag gttgataata gggtcgagct
    10921 tgatgaagat ggaattatcg atgtaatagc aaaacaatta aagcaacgta gagatgcatt
    10981 agttgaattt gaaaaagctg gaagagaaga cttaataaaa gaaacagaag gtgaaattga
    11041 agttttaaaa gagtacctac ctcaacaatt aagcgaagaa gagttagaag aaattgtaaa
    11101 atctactata tctgaagtag gggcaacttc tatgaaggat atgggaaaaa taatgtcagt
    11161 tatccaacca aaagttaaag gaagagctga cggaaaactt ataaataagt tggtaaaaca
    11221 aaacttacaa tagattgata tagcctagag agaaatctct aggcttttta tagtttttgg
    11281 gaataacgag tcatatttga gcatatctat aatcaaagaa aattttgaaa gaaggggaaa
    11341 aatttgatta tatcagtaaa agagattagg aattttttga taaacatatt tataccacta
    11401 ataataggtt acttaagtag catcttaaca atgattatca gtggaacaga tatttcaacc
    11461 tattatttac aactcacaaa gccaagcttt gcaccaccag cttttatctt tcctatagtt
    11521 tggactatat tgtacatatt gatgggtata tcttcatacc taatattaag aaagggttac
    11581 aacttaccta aagtaagaga tgcaattttt tattatggat tacaacttgt attaaacttt
    11641 atttggagta tactattttt tggatttgga cttagattta cagctttgat agatataata
    11701 ataatgattt tggtttctat aataatgatt tcgaagtttt ctaaaataga taagagatct
    11761 ggatacataa atttgattta tttaatgtgg cttgtttatg caggattttt gaactatttt
    11821 atatggttta ttaataaata gaatgtaaag attttgtaaa ttaccctgta cttttaataa
    11881 tatcataaca aacgaaaaat aaatagacta ttaaaaataa tcattttcat atacaataat
    11941 attaatttaa atacaataaa tttataaaaa ttgtatttaa agattaacat attaaagtat
    12001 tagaattgga aaggcttgaa gggagaggtt tttatgcaaa taatagtatc tgggagacaa
    12061 atgaaattaa ctgacggaat aaaaggatac gtagatggaa aattaagtag gttagaaaaa
    12121 taccttgatc ctgaatcaga agtgaaagta acagtaagtg cgaaaaagga tagacaaaaa
    12181 gtggaggtta caataattcc tattaacggt caaataataa gagcagaaga cgtagaagat
    12241 gacttatatg cagcaattga tattgtttgt gacaaattaa gtagacaagt tgtcaaatat
    12301 aaaactaaag ttaaagacaa agttcaaaat aataagagca taagatttga aaatcttgac
    12361 tttatagata atagcagtga atttgatgat tatgattatg atgatgaaga agatgagaat
    12421 atagtaatag aaagaagaaa gaaatttaat gtaaaaccta tgagttctga ggaggcgata
    12481 cttcaaatgg aactagtagg acacaatttc tatatgttta gaaaccaaga taattttgaa
    12541 ataaacatag tgtataagcg taaagctggt ggatatggtt taatagaaca agattaataa
    12601 aaattgtata cgtttagatt ttatataaga aaaaataaaa aggtaatcat gttgattttt
    12661 tatcaatatg attacctttt tcttgtttaa ataatatatt agatattagg tatttattat
    12721 ataaaaattt taaaactaat ctgttacaac atcaataaag tgcataaata cctatttcaa
    12781 gcataaaatt aattaagggg gtaatgctta tgttagaaat atccactgat ttacaagtaa
    12841 atcaaccagt tattactgtt acaagtaata cgtttattag tatagaaaat tatctatcaa
    12901 tattagaata tgaagttgat ttaataagaa taaagactaa agttaaaaca attaaaatat
    12961 ctggggataa actttcactt aagtatatta ctgatagtga gataggtata aaagggatta
    13021 tctacaatgt tgagtatgtt gattaggagg agatacactt gataagtttt ataaggggat
    13081 actatgttat agttgtggaa ggagtaggac tcgaacaatt tttaaatcat cttataagaa
    13141 atgggataag tgtttacaat gtaactagaa taaaaaatac taaaatggag tttcatatag
    13201 atagacaaga tataaaagaa tttaagaatg tttatagagg aagtaagttt gatataaagg
    13261 taaagcaaaa aactggggtc ccttttatta tcaaaagagt ttataagcac aaaggtatgt
    13321 ggatttgtgc tcttgtttct ttatttttat tgatgtctac atcacagttt gtgacagatg
    13381 tatatataca atctcctgaa ggtataaaaa aagaagctct tagaaatgag ctttataagg
    13441 ttggagtaag accaggagtc tataagaaga gtattgatag aaaagaagtt agagaccata
    13501 tgatgtcaaa atttaatgat gtggcatatt tatctatcaa tgtaaaagga acaaatatat
    13561 ttgttactgt tacaaaaaag gcagaatccc taaaatctac agaccaatca aattattgta
    13621 atgtaattgc tttaaaaaat ggtataatag aaaaggtaat ccctagaagt ggtaaatcag
    13681 ttattaaatc tggagacata gtccaaaagg gagatgtctt attaaatgga gctaatacca
    13741 agtcaatacc agaagtatgg gcaagtactt tttatgagtc cacaaaaaag gcaagttatg
    13801 tagatactgt aaataaaaaa actggagaaa agaaaaatat atatacacta agtttttatg
    13861 ataaagagtt tacaatgaga aaaaatataa aatataaaga ttatgttgtt gaaaataaag
    13921 aaaaaaaact atcaatagga aactatactt ttccaataaa gataaaaacg agtacttttt
    13981 atgaaacaaa aaaggttgaa gtaaaaagga ataaagaaga gttaaaaaaa gaattatctg
    14041 aaaaagcttt aaaagagtta gaatacataa tcccagcttc ggctagaata atagatgtaa
    14101 aacataactt taaggttaat aaaaatatgt tagaatattt aataacagtt caaactagtg
    14161 aaaatattgc taaaatttat cctcttagta agtcagaagc agaaaggttt ataaaggaag
    14221 aaagtaaacc agatgaaggt gaagaggaag ttccttccaa tccagaaaaa agaccattaa
    14281 atgatataag aaatgagttt gatgaggata ataaggataa taaagatcaa aacaattctg
    14341 atgaaaataa tagtaatcaa aatagtaata atagtcaaaa taataatagc aataacaact
    14401 aataaaatta aacttaaaat cttaggagga ctaattcaat tgataataca aaagaaattt
    14461 acaatagcag atggtaactt tgaaagagag ttatttggca actttgatga aaatgtaaaa
    14521 ttaatagaga agactttaaa tatagatgta atacttagag aaggcaatat aattttaatt
    14581 ggagaagaaa aaaatgtaga ttctgcatta aaacttatga atgaattaca ccaaactgta
    14641 tcaaatggta agcatttaga taagcagagt atttcttatt cattatctct tttgcttgaa
    14701 ggaagtgaac aaaaaataaa agaacttgag gggacaatcg tcataactca aaggggaaaa
    14761 gctgttcaac ctaaaacttt aggtcaaaaa gaatatataa aacttataga aaataatgat
    14821 ataacttttg gtgttggacc tgctggaaca ggaaagactt atcttgcggt tgctatggca
    14881 gtaaaagctt ttaaaagaga tgaagttagt agaattatac ttacaagacc agctgtggaa
    14941 gcaggagaga gtctaggttt tttaccagga gatttaaagg acaaagttga cccatattta
    15001 agaccactat atgatgcctt atttgaaatg ttgggtgctg ataaatttaa taagtattta
    15061 gaaagaggta ctattgaggt tgcaccactt gcatttatga gaggtagaac acttgataat
    15121 tcatttataa tattggatga agctcaaaat actacttctg aacaaatgaa aatgttttta
    15181 actagacttg gatttggctc taaagctgta gttacaggag atgtaactca aacagattta
    15241 ccacaaaata agaagagtgg tctaatacaa gctacagaga ttcttaaagg tgttccaggt
    15301 attgggtcta taatgctaac agatagagat gttgtaagac atgagttggt acaaagaata
    15361 ataagagctt atgagaagca cgacaaaaga gaagaattta aaaaagaaga acgtaaaaaa
    15421 gcgaaaatgc aggaaaagac tttcaaaaga aagtaaaagt gtagtaatat agatatatag
    15481 cattattaat gaataaaaag taggtgtagt gaatggattt gatactagat gatagacaag
    15541 ataaattaga agtaagtgag gagttaattg aaaagataaa ggatataatc atagaatgtt
    15601 tagattatga aggatatgat gataattatg aagtgagtct ttcttttgta gataataaag
    15661 agattcatga attaaataga gaatatagag gagttgatag agtgactgat gttttatcat
    15721 ttcctctttt aagtgatgat tttgaggatg ttgagttaga agaagaatcc ctaggtgaca
    15781 tagtagtatc tcttgaaaga gccttagaac agagtataga gtataaccat agctttgaaa
    15841 gagaagtatg ttttttaata tgtcatagta tgtttcatct tttaggttat gaccacgata
    15901 cagatgaaaa tacaaaagaa atgagagaaa aagaagaaca catattaaat aagttaaaca
    15961 taacaaggga gtagttttta tgaaaccaga aaaaactcgt caaggtataa ttaaagcatt
    16021 taatgcagct atagaaggga tattgtatac atttaaattt gaaagaaata tgaaaataca
    16081 ttatttaggt tcagttgctg ttcttataat aagtttgttt tttaactttt caaaattaga
    16141 aatgataatg ttacttatgt ccatatgctt agttgtagtt gctgaaatgt ttaatacagc
    16201 tatagaaaaa gctgttgatt tagtaactga tgaatatcat gttcttgcta agatagcaaa
    16261 agatgttgca gctggaggcg ttctagtggc agcactaaat tcagtagtag ttggatatat
    16321 tttgttttat gataaattga cagatatatc tggaatatta atttataaaa taagagaatc
    16381 agaattacat ataaccttga tatgtatatt attggttctt atagctgttg tagttgtaaa
    16441 agcattgact tctacaggta ctcctctaaa aggaggaatg ccaagtggtc atgctgcctt
    16501 agcatttgct atagcaactg caattacact tatgactgaa agagttgttg cctctactct
    16561 tgcatatata atggctgtat tagttgccca aagtagaatt gaaggaaaaa tacatacctt
    16621 ttgggagact attgctggag ctttgttagg tgtgttgata gctatattag tatttcagct
    16681 tggaatgttc tataattaaa ataaatgtac ataaaatatt taaaataaaa caaatctgtg
    16741 ctaattaaat ttttatgata taatgtagag tattaaataa aaataacttt taattattgt
    16801 atacaggagg agttgttgtg gataacatag aattgttaag gttggcagaa gatgccagac
    16861 aacattctta tgctccttac tcaggtttta gagtaggtgc agctctttta actaagagtg
    16921 gtaaagttta tactggatgt aatatagaat gtgcatcatt aggtggaaca aattgtgcag
    16981 aaagaacagc tatttttaaa gcaatatcag agggtgataa ggatatctat aaaatagcta
    17041 tagcaagtga taactcagaa aataatgagc aaacatatcc atgtggaata tgcaggcagg
    17101 taattataga gtttggttct gatataaaaa taataacggg atatactaaa ggagaggtgt
    17161 ttgagcatag tataaaggat ttgttgccaa actatttctc tggaaatgat ttaaaataaa
    17221 tttaaaagta taaataaatt taagtaatta aaacaagaag ataaaaagtt aggagacagt
    17281 taatgttcaa atcaggtttt gtcagtatag tgggtagacc aaatgtagga aaatctactt
    17341 taatgaataa tgtagttgga gaaaaaatag ctataatgag tgataagcct caaactacaa
    17401 gaaatacaat acaagcagta tatacagatg aagaaatgca gatagtattc ttagatacac
    17461 caggtattca caaaccaaaa aataaattag gtgaatttat ggtaaaggca gcaacagaag
    17521 cttttaaaaa tgtagattta atattatttg tagttgatga ttctaaaaaa ataggtcctg
    17581 gagatagaaa gataatagag gatttaagaa gtgttaagac accaataatt ttagttgtaa
    17641 acaaaataga ccaactagac caaaaagatg agctatttga tataatcaaa atgtatgata
    17701 gagaaggcat ttttaaagaa atagtaccaa tatcagcatt aaaaggaaag aatacggata
    17761 ccttaataaa agttatacaa aattatttag aagaagggcc aaagtatttt ccagattata
    17821 tgattacaga ccagccagaa agagtattaa tagcagaact tataagagaa aaagttcttc
    17881 actatcttaa tgatgaaata cctcatggtg tagctgttga aattgaaaaa atgaaagcga
    17941 gaaatgataa ggaaatagta gatgtatctg ctgtaatata ctgtgaaaga gattcacata
    18001 agggaataat aataggaaaa aatggtagga aactaaaagg aataggtaag tcagcaagac
    18061 aagatataga attgctttta ggttcacaaa taaatcttca attatgggtt aaagtcaaag
    18121 aaaattggag aaatttacaa aattatataa ataattttgg atataatgat aaataaaggt
    18181 gggttatatg gataggaaac tggatgcttc ccaagattta ttatatgaag taaaatcatt
    18241 aattgataat aataaagtat tggagttaag agaattaata gaggaatatc atataattga
    18301 tatattcgat ataatggaaa atttagagga agacatgaaa attcaattat ttgaagtact
    18361 tcctttagat atggcatctt ctatattaga agaaggtagt gtagagtttt ttattagtat
    18421 tttatctaaa ctggatgtag aacactcgaa aaatatactt gaacttatgt ctcttggaga
    18481 tatggcagac aagttgagtg aacttgaaga agaagaaaga gaacacataa taaatctttt
    18541 gaatcaagag aatgccgatt atgtaaaaga gttactattc tatgatgaag attctgctgg
    18601 tggaacaatg accactggat atatttctat aaataaagat atgacagctc ttgaggctat
    18661 agaccatatg agagaagaag ctgaagaagc tgaaactatt tattatatat atgtggttga
    18721 tgatgaagaa aaactagttg gagtattgtc attaagagaa ttaataatag ctagagacgc
    18781 aaatatagta gaagatttga tgagtgaaaa tataatctct gtttatgtag atgaagatag
    18841 agaagaagca gtaagattag tatctaaata caatttaata gcaattccag tagtagacag
    18901 acaagaaaaa ttaaaaggaa taataacagt agatgatata attgatgtaa tggaagaaga
    18961 agcaactgaa gatatgtata aatttgcagg aagttcagaa catgaaagag aagttgctga
    19021 aaaagaaaac cctacattga gagagcaaat aatatcagca cttagaggac gattaccttg
    19081 gttaattatt accttagttg gagggttgtt ggcttcgtta atactttcta acttagacta
    19141 tataatgaat cctgtatatg ctccattagt attttttata cctgtagtaa ttggtatggg
    19201 tggaaatatt ggaactcaat catcatctgt tacagtaata acattatcta ataaggattt
    19261 aaattttagc aatgtggtta gagaaggtat tgtaggtatt attacaggtc ttttgtgtag
    19321 tataataaca ggtattgtaa tttactttgt tatgagaaag ttagacattg tattaattgt
    19381 ttcaatatca ttatttataa atatggtttt aggtgcaaca ataggtgcgt ttatgcctgt
    19441 tttattaaaa aaaatggatg cagacccatc tacagtttca tcaccaatta tatctacagc
    19501 tcttgatata actggtatag cagtttactt tataataaca acggcattat tgtcaaaaat
    19561 tgtttaataa ctttatgtat gatttatcct cttcttacta aaaatataag tataaatgta
    19621 agaagggggt tttttaaaaa tgaacaatat cagttgggaa gtttttaaaa aaacaggtag
    19681 tatagatgca tatttatatt tctgtgattg caaaaacata catgaagaaa ttgaagaagt
    19741 aagggagaaa aaagaagacg atgataatcc tgaacaccca ggggatagta cttaaagcta
    19801 taaggtataa ggaaagtgat ataatactta cactatttac aagaaagctt ggaaaagtat
    19861 ctgcaatagc aaaaggagct aaaaaaaata agagctcttt gctttcatca tcacagttat
    19921 tttcttatag caattttacc ttaaaaaaac aaggtaatat gtacaaggtt actcaaagtg
    19981 aaataataaa aagtttttat aatatatcct atgatataga ggcattttct tatgctacat
    20041 atattacaaa attagttgaa aattctatac ttgaaaatca gactaataat aggctattta
    20101 tattacttgc gcagactctg tatttgtata cacaggataa tacagacaat aggtttataa
    20161 cagcagcatt cgagttaaaa tttttagact atataggatt taaacctata gtaaataaat
    20221 gcattaattg tgaatgtaaa attttacaaa attctgtgtt taatatctac gaaggtggta
    20281 tactatgtag taagtgtagt aaactttttg ataataatat aaaattagac ttaacaacta
    20341 taagtcttat ggaatatgta ttaagaaatg atattttaac atgtagtaaa gctaaagtat
    20401 caaaatatat aacacatgaa cttgagaata ttctagacaa gtatttgaaa gtttatgttg
    20461 ataatattaa ttttaaatca ttacacgtat tgcaaagtgt aaaaaataat aagggagtgg
    20521 ataatgatga gtagtaatat aactattgaa atggttgatt cagtctttga aagagttcca
    20581 ggagcaactt atgcagaagc aaaagaagca ttagtaatgt gcaatggaga tgtaattgag
    20641 gcagtaatat atttagaaaa taaaaaaaat acttgtaatt gtaaagcaaa atctgctaaa
    20701 gaaactatgg aagaagtatt tggcaaagat ggagaaaata taaagtttca attaaaagag
    20761 cttcttagaa aaagtagtgt agtgagaata atagttgaaa aagatggaaa aactatgatg
    20821 aatattcctt taacagttgg agttgttgga ttagcttttg taccattagc tactttagtt
    20881 ggattatcag ctgctgttat aagtaaatat cgtataaaag ttcaaaatga agaagatgga
    20941 gaaattgtag atttaggtga attgaatgaa gaaaaacttc acgttttgaa gaatatgata
    21001 actaatgcag caaaagatgt gaaggatgta gtagttgaca aaaaagaaga tgacaaagat
    21061 gtaactgctg accttatgaa agaagaatct gaaaagaata aagaagacga agacgaaaat
    21121 aaatagatat aaagtgactt ttaaaaaatg aagagaagat aattataaga attatcacta
    21181 acattgacaa attttataga atttgatatt ctaaaagttg tagaagaact attaaaatag
    21241 cctttcgtgt agacgagagg ctttattgtt ataggaggga tttttatgaa ttttcaagag
    21301 atgatactgg cgttgcaaaa atactggtca aagcaaggtt gtatcatgat gcaaccttat
    21361 gatattgaaa aaggtgcggg aactatgaat cctaatactt ttttaagatc attaggacct
    21421 gagccttggc aagtatgcta tgtagagcct tcaagaagac cagcagatgg aagatatggt
    21481 gaaaacccaa acagactata tcaacatcat caattccaag ttatactaaa accttctcca
    21541 gataatatac aagaattata tttggaaagt ttgaaagaga ttggaattga cccaagtgag
    21601 catgacataa gatttgttga agataactgg gaagctgcta cagttggtgc ttggggactt
    21661 ggatgggaag tttggctaga tggtatggaa ataactcaat ttacgtattt tcaacaagta
    21721 ggtaatattg aatgtgagct tgagacagga gaaattacat atggattaga aagattagct
    21781 atgtacattc aagaagttga tagtgtttat gatttaaaat ggaatgataa aataacttat
    21841 ggtgaagtat ttaataaagc tgagtatgaa aactcaatgt acgcatttga attatgtgat
    21901 gcagatatgt tgtttaatct gtttgatata tatgaaaaag aagcattaag acttatggaa
    21961 aatggccttg taataccgtc atatgactat gtgttaaaat gctctcatgc atttaataca
    22021 ttggatgcaa gaggtgcaat aggagttagt caaagagctt catttatagg tagagttaga
    22081 aatatggcaa agactgttgc agaaactttt gtaaaacaaa gagaagaaat gggattccct
    22141 cttttaaagg acggtgacaa ataatgaaaa attatctttt atttgaaatc ggtgttgaag
    22201 aaatgccatc aagatttgta ggcagtacac tagaacaatt aaaaaacaat cttagtaaat
    22261 tatttgatga aaatagaata gcttttgata aaataaatgc ttatgggaca ccaagaagac
    22321 ttactttagt agtcgaagga ttgagtgata gacaaagtga tttagaagag gaaataaagg
    22381 gtcctgctaa aaaaattgct gttgatgcag aaaataattt aacaaaacca gcattaggat
    22441 ttataaaaag caaaggatta agcgaatctg acatatattt taaacaagtt ggaaaagaag
    22501 aatatatatt tgcgactata aagcaagatg gaaaagaaac ttcagaagta ctaaaagaca
    22561 tattaccaga gactataaaa tctgtagtat tcccaaaagc tatgcgttgg ggaggaaaaa
    22621 atcttaaatt tgcaagacct ataagatgga tagttgcatt gttaaatgat aaagtattag
    22681 agtttgattt agaaggaata atatcttcta atgtaacaaa aggtcataga tttttaggtg
    22741 aaagtacaat agaagttgat tctatagaag attattttga aaaattagaa aaaaattata
    22801 tagttttaga ccaaaacaag agaaaagaaa tgataagaaa acaatgtata gaagtagcaa
    22861 attctttagg tggagaagtt gaatttgatg aagatttact tgaagaggta acttatttag
    22921 ttgaatatcc aactgctttt tatggagaat ttgatgtgga ttatataaaa ctaccaaaag
    22981 aagttgttat aactcctatg aaacaacatc aaagatattt tccagtactt aaagaaggaa
    23041 aactgttacc taactttata gctgttagaa atggtgattc ttacagaata gacaatgtta
    23101 aagctggaaa tgaaaaagtt ttagaagcaa gacttgcaga tgctttattt ttctatagag
    23161 aagatactaa gagaaactta gaaagctaca tagaaaaatt aaaaactgtt gtattccaag
    23221 taaaattagg aactatttac gacaaaactt taagacttga aaaattaagt gctgatatac
    23281 ttgatagttt aggtttagta gatgaaaagg cagatactat aagggctgct aaattatcta
    23341 aggcagattt agttactgga atggtaggag aatttgatga attacaaggt tttatgggaa
    23401 aagaatacgc aaaagttgga ggagaaaatg atgttgtaag tgaagctatt tttgaacatt
    23461 atcttccaag atttgcagga gatatacttc ctaagactaa tgcaggggtt gccttatcaa
    23521 tagctgataa gttggattca atagcaggtt tctttgctat aggaatacaa ccaacaggtt
    23581 ctcaagaccc atatgcttta agaagacaag ctttaggtat acttagtata cttatggaca
    23641 gaaaagttgc tgtggatata aaaatactta ttgaacatgc tttagacaat tattctgaat
    23701 tagattttaa taaagaagaa gttgttgagc aaataatgaa tttcttcaat gagagagtaa
    23761 aaaatctatt taaagattta ggaataagat atgatgttgt tgatgctgtt ttaagttctg
    23821 atataaatga tgtttctgat atgcatatga gagctttaga gcttaataat tggctagaaa
    23881 aagatgaatt agtggagatg cttactgcat ttaatagagt ttctactcta gctcaaaaag
    23941 cagaaagtga taaaattgat gaatctttat taaaagaaga tgctgaaatt aaactttaca
    24001 atgaatttaa acaagtaaag gtaaaagtta atgaattatt aaaggataag aaatatggta
    24061 ttgctttaga tgaatttgca tctttaagac cagtagttga taatttattt gatacagtta
    24121 tggtcatgga taatgatgaa gctattaaga acaatagact tgctttactt aaacaagtat
    24181 atgatactat gctaggaatt tgtgatttat ctaaaattgt ttataaataa aatttaaact
    24241 atttataaga aataaggctg tctcaaaata gagatagtct tatttttcat tgttgagatg
    24301 atgaaaatat atttgatttc atagaaaaag agtgtctcat aaactaaaaa gttcattttg
    24361 agacacccat gttttttgta agtaaatata ataaattata ttttatactt atcagttatt
    24421 ttgtttaaaa tattatcatt tgaatttacc cattcataaa aattgcctga agatgcgtcc
    24481 gatattttat aaagcatttc aggaatatca ctagctttaa aatctccaag tgattttccc
    24541 aatctagtct tacctatttc aaaactgcct ccaacaaata attcaaatgc atctgtagat
    24601 ataccatcaa cattcttctt tttaccacat aaacctatgc taccaacttg atgaacacca
    24661 catgaattta aacatcctga tatatataac ttaggtgctt tatttaaaat ttcttcatta
    24721 ttttgtaatc taaaataatc tatgatttca tgtaacattt tctgactatt ttgtatgccc
    24781 atttgacaaa ttggaacacc tatgcaagat atactttgtt ctaattgaga attacaacta
    24841 atagttttac ttatttctaa tattcttttt gcttcatttc catttaaatt taaaatatat
    24901 aatccctctg tcattcctag tctaatcata ggattttcaa cattatctaa ttctttaagt
    24961 aaagtactta aatcctttgt taacaaaata cctccaactg gatgtatata tacagtgtat
    25021 aaaccatcct gtttttgagg tattagtcta gaatcttgta tatctatttt aactccaggt
    25081 ttagagtaat ctatttcttc aatattgagt tcaagactac cattttcttt ttctaattta
    25141 tagtattctt taaatttttc taagaatagt tcttcaccaa gtgattcgac catatatcta
    25201 accctagctc tgtttttatt ttcataattt ccataatcca taaagaattt aatcatacct
    25261 tcaacacaat ataaggcgtc tttcggcttt ataagttcat caagttctaa agcaactttg
    25321 ggttgtttac ctagacctcc acctaagaaa actctgaaat atggttcatc atttttcaaa
    25381 gtagcaacaa atcctaagtc ttgtatacta caatgagcag tatctgtgta acaattagaa
    25441 taagaaactt ttaattttct aggtaaatgg tatgtagtag catttttgat aaaatattta
    25501 tcagtagcaa ctgcatatgg agtaacatca aagacttcac tagggtcaac tccagataat
    25561 ggagataatc cgacatttct aggataatta ccaccaccac ctctagtgaa tatatcattt
    25621 tttattcctt cttccatgat atcaacaatt gaatttatag ataaatcgtg taattgtata
    25681 gcttgtctag ttgttaagtg tatcttatct aagttgtgtt taatagctat ttcatagatt
    25741 ttattaagtt gatactgaga taaaactcca gcagatactc taagtcttat cataaaagat
    25801 tttttgtctc tttgagcata aacaccatat ccaccagaaa atcccttata ttgcatttta
    25861 tttatttctc catttaaaaa ttttgaagaa tattctttaa aattaggtag ttcatttaac
    25921 aatatctttt taaaattatc cattatttca cctcattgtt agtcttgtat aaaaatatat
    25981 ctattgtata atagatatat atattattat ctagtcagga taaaaatata acagaataat
    26041 tctattaaaa gtaagaataa taaggacata gtgagtattt tgtttttatt ttacaaatta
    26101 agtataatat ttttgtttta taaaatataa ttaagataaa tagataattt tgtaatttta
    26161 atatataata tttagtagag tgtgggagta gcacttaata tttgcaggag gagtttttgt
    26221 atatgggaga aataataaat gcattagata gagccctaga cataattttg ctgttatatc
    26281 atgaaaagag agaaatggga ataactgaaa taagtaaggc tatgggagta tataaaagta
    26341 ctgtacatag aactttggtt actcttgaaa acaaaggttt tgttatacaa aatgcagaga
    26401 atggtaagta ttggctagga ataaatttat atgcaatagg tatggttgtg ggagaaaaaa
    26461 tgtcattgac tgaaatagta aaaccatata cgaaaaaatt aaatcaagaa tttaacgaag
    26521 tagttaatgt atctatatta gaagaaagag ctcaagattc tccaagaagt atcataatac
    26581 ataaagagta tggttcaaat caattacttt cagtaaatcc atccgttggt tcaagtagtg
    26641 aatgttattg ttcagcagtt ggtaaatgtc ttatggcatt taatgattct atagattttg
    26701 aaaaatatag aaaaacacca atacacaagt atacagaaca tacaattgat aattgggatg
    26761 atatgatgct ttttttagca aaaataaaag aacaaggata tgctatagat gatgaagaac
    26821 tagaacatgg actaacttgt attggagcac ctatcttaga taaaaataat aaagctattg
    26881 cagccataag tttgtcaggt ccaactataa gaatgagaga aggggacttt gagtataaga
    26941 ttaaaagagt tatagaaact gcaaaaagta tatctgaact ttttagatga tttttaatct
    27001 cgataataaa aataggctat atttaaattg ttatttgtaa atatcagttt aaatgtagcc
    27061 tatttttctt taatctaaaa ttaaatattc ttaaaattta aaatgttatt gaaatatata
    27121 agcgaatgtg ttatctttta attaagcaat aaaaatagaa caaggttcta tactatagaa
    27181 caaaattgat gcatatatag tatttataca aaatataaat attatatatt taaaattaag
    27241 taaaaatatt acataaaggg gagatttgag aaatggaaaa agtcaagttg agtgttgaaa
    27301 gatgtaaggg atgctatctt tgtattgaag cttgcaaaaa agatgcaata agtctttctg
    27361 actacacaaa taaaaaaggc tttgtaacta ttaaagtcga tgaagaaaaa tgtattcaat
    27421 gtggcgcttg ttatacaatg tgccctgatt tagtatttga gatattataa attattttta
    27481 ttagagggga gaaaaggtat gcaaagttta ttaattaaag gaaatgaagc tttagcagaa
    27541 ggtgctatac gtggtggttg cagattttat gcaggttatc caataacgcc acaatcagaa
    27601 atattagaaa tgatggcatg gagacaaaaa gaagttggag gagtatttat ccaaggtgaa
    27661 tcagaaatag ccagtgtaaa tatggttatg ggagcaagtg cacttggagc aagagctatg
    27721 acttcttcat ctggtccagg attttcatta aaacaagaag gtattgcata ttgggtatca
    27781 gctggtttgc cagcagttgt agcatgtgtg caaagatatg gtataggtga tggatttata
    27841 ggagctggtc aagataatta ttggcaagca gtaaaaggcg gtggaaatgg agattatcat
    27901 ttaatcgtgt tagcacctaa tagtgttcaa gaaaatatgg atatggcata tgaatctttt
    27961 gaattggctg aaaaatatat gcatccagta cttatacttt ctgatgctac aataggtcaa
    28021 atggtagaac cttgtatagc accaccaatg aaagaatacg atatggataa agctccatgg
    28081 gctgcaaaag gaacacctgt aggagaaaaa aataaagtaa taactgatat aacatatttt
    28141 gaagaccaag atacatggca aaaagggtat atagaaaaaa tgagaaaagt tcaagaagat
    28201 gaacaaagat gggaagagat agaaatagaa gatgctgaag tggtatttgt ggcatacggt
    28261 atttcatcaa gaatagcaga aggtgcagtt agagatgcta gagcaaaagg aatgaaatta
    28321 ggtctaataa gaccaaaaac attgtggcct tacccaagaa aagcatttaa gaaattaagt
    28381 gattcattga agggacttgt aacagttgaa atgaacatga tggcacagat gaaggaagat
    28441 gtaattatag catgtgataa tagatttcca gtatattcag tagcaacagc tcaatatatg
    28501 gcagaagtaa atcaattaat agattttgct caacagatta tagatggaaa agctaagcaa
    28561 gaggaggtat tttaattatg gctaaaaaag caccagcaat aatgatgtgt gaaaatatgt
    28621 tctgtggagg ctgtggacac tctatagtcg atagaattat tggagaagta ttaacagaaa
    28681 tggatatatg tcaagataca attatatgtt cagatatagg ttgtaatcat atgtttcaat
    28741 ttagtatgaa tgtagatgtt gtcgttccac ctcatggtaa aatgggagca gcaatggcag
    28801 ctatgaaaag agttagacca gataaatata tatttacaat agctggagat ggtggagctt
    28861 atgcaatagg gcttggagaa actatgtcag ctgctactag aaatgataat gtaatatttc
    28921 ttgttatgaa caataatatg tttgcaatga caggagggca aatggctcca actactatag
    28981 aaggtcaata tacagcttct actccagcgg gtagaaatta cgcagatcat ggttcaacat
    29041 ttgatgttgt aaaagttatg ggaaatttag atatagcata tctagcaaga ggaacaataa
    29101 ctaaccctaa agaaataaat acaacaaaaa gatacatcaa aaaagctcta gaaaaacaaa
    29161 aagaaaagaa aggtttttgc ttaatagaag tgcttgtacc atgtccaaca aactggggat
    29221 taaagccagt agatgctatg aagagaattg aaaatgaact tgttccaaag tatccacttg
    29281 gagaatttat tgattaggga ggaaataata tgttgaatga aattatatgt gctggttttg
    29341 gaggacaggg agttcttaca gcaggaaaaa ttataatgta tgttgcatat aaggcagaaa
    29401 agaaaattac atggttccca tcttatggaa atgagatgag gggaggaagt gcaaattgca
    29461 atgttgttgt aagtgatgaa aaaattgcaa atccttgtgt aaaaaatcca gatatactat
    29521 tgggaatgaa cagcgtatca gtagataagt atgaaagttt aatgaaaaaa aatggttata
    29581 tgtttgtcaa tgaatcacta gtaagtaagg atagaaagta cagagaagac attaatgtta
    29641 taaaagtacc tgttactgaa atagcacaaa atttaggcaa tgaaaaagct gctaacattt
    29701 gcatgttagg tgctatggta aagagtttgg atttatttac aaaagaagaa tttgaaagtg
    29761 gtatgtgtga atactttgaa aatcaaggca aaggtaaatt caattctaaa aatgtagaag
    29821 catttgaagc tggatacgac tacgaatgaa tattttaaat atttaaaata attattgaaa
    29881 tacttatttt tatatgttag tatttaatta ataaaatata gaacgtcgtt ctgaacaata
    29941 ggacgaagtc tttaggtcgg aatacaagaa cgacgttctg tatagtagaa ctagggggtg
    30001 ttaaattgaa agttttagta ataaatcctg gtggtacatc cacaaaaatt tctgttttcc
    30061 aagatgaaaa tgaaattttg aaaaaaaata taacccatac aagagaagat ttaaaaaatt
    30121 tttctaaagt atttgatgaa tatgattata gaaaacaact gatagtagat attttaagta
    30181 gtgagaatca tagtataaat tcttttgacg cagtagttgg tcgtggtgga cttatgaagg
    30241 ctataaaagg tggtacctat actgtgagtg aagaaatgat tgaggatatg agaaatgaaa
    30301 taaatgggga acatgcatca aatttaggtg cattattagc taaaactata gctgatgaaa
    30361 taggagtaca atcatttgta gtagacccag tttctgtaga tgaatttgat gatgtatcga
    30421 ggattacagg aatatcagac atagaaaaag caagttggtt acatgcatta aaccataaag
    30481 ctgtttcaag aaaaattgca caaaaactag ggggaaaata cgaggattat aactttattg
    30541 tagctcattt aggttcagga atatcgattg tagcacataa acaaggcaaa atgattgatg
    30601 gaagtggtgg taggtctgat ggtccatttt ctccagaaag gagtggtgga ttacttgcat
    30661 atcctcttat agaattgtgt tactccggaa aatatactca tgatgaaatg gtcgcaaaag
    30721 taagttcgat tgggggaatg tacgattatt taggaacaaa ggatatgata gaaattgaaa
    30781 acagaatcaa ggagggtgat gaaaaggcaa aacttataat ggatgcattt atttatcaag
    30841 tagcaaaaga aatttctatg tatggagctt cactttcagg gaatgtagat agagttatct
    30901 tgacaggagg gatatctcac tcagaaatgg tagtcaaagg agtagcagaa aaagtttcat
    30961 atctagctcc aatagagata gtaccaggag agatggaaat ggaggcactt gctctagggg
    31021 cacttagagt acttaatgga gaagaaatag caaaaactta ttaatattta ggagaaagag
    31081 tatgttaaaa gattttaata atttattaga agaggctaaa aagcttgaac ctgttattgt
    31141 aagtgttgct gttgcagaag acaaagaagt attaaaggct gtgaaatcag caacagattt
    31201 aaacattata aagccaattc ttgtaggaga caaggataaa atagaaagta tatcaagaga
    31261 aattggatta aaaggtcatg aaataatagg ttgtcaaaat cccgatgaat cagttaaaaa
    31321 agctgtagag atagttaaaa ataaggaagc taaagttctt atgaagggat tagtaaatac
    31381 aagtgtctat atgagagcta tattaaataa agaaacgggt cttagaacag gaagattatt
    31441 aagtttgctt gcagtatatg aattacctca atatcataaa cttctttatt gtactgatag
    31501 tggaataaat gtatcgccaa acttaaatca aaaaaaagat ataatgacaa atgcacttct
    31561 tactatgaaa agtataggca tggaaaatcc taaaactgcg atacttactg caaatgaaat
    31621 ggtagaccct aagatacaat ctacagtgga tgccaaccta cttgtagaga tggaaaaatc
    31681 gggagaaata ccaaagtgta tagtggaagg tccaatagca tttgatgtag catttgatgc
    31741 acatgcagca gaacataaag gtattaaaag taatattgct ggtgatgttg atttattagt
    31801 atttccaaat atagaagcag ggaatatatt aggaaagtca tggcttagat ttaataaagc
    31861 taagtgggca ggtatagttt taggagcaac aaatccgata attttaggtt cacgttctga
    31921 cacagctgaa ataaaaataa attcaatagc attagcgtgc ctagcatcac aataagaaat
    31981 tattattaag gaggaaaaag tattatgaaa tatgcaaaaa gaatggatat ggtaaaagct
    32041 tcagctataa gagattctca aaagaaaata gctcagaagg tagcaagtgg tggaactgtt
    32101 atatcttttg ctgcagggct tcctgaccca aatttatttc caatagaaga gataagtgaa
    32161 ataactcaaa aagtacttaa tgaaagggga aaatttgccc tagcatatgg acctactaaa
    32221 ggtgaagatg agttgttaga tctattagta aaaagaatga aagaagaaga aaatattgat
    32281 acaaaagcag aaaatattat aataactact gggtcacaac aagggatagc attaagtgct
    32341 atgattttat tagaaaaggg agatgtagta gttacagaaa atccaagtta cttaggtgca
    32401 ataaatgcat ttagacctta tgaatgtgat tttttaggag tagatacaga tgaagaagga
    32461 atggtaacag atgatttaga caaattatta tctgaaaatt ctaacataaa ggtgatatat
    32521 gtaataccaa catttcaaaa ccctacagga aaaacatggt ctttacaaag aagaaaagat
    32581 tttatggaag tcgtgaataa atatgatgtc atagtaatag aggataatcc ttatggagaa
    32641 ataagattta caccagaacc tctacctaca cttaaatctc ttgatactaa agataaagta
    32701 ttgtaccttg ggtcattttc aaaggtatta tgtccaggat ttagagttgc ttggatgtgt
    32761 ggtaatgtag acatcataga taaggcagaa cttttaaaac aaggtataga tttacagtca
    32821 aatcaatttt cgcaagtaca agtaatagaa tttttaaaga aatacaactt aaatgaacat
    32881 atagaaaaaa taagagcaga gtataagaaa agatgtctgc taatgttaga tgctatgaag
    32941 aaacatttcc cagaggaagc taaatataca gaaccagatg gaggaatgtt tatatgggtt
    33001 gaacttcctg aaagtataaa tgtagatgat ttattagata aagccattga tgctggagtt
    33061 gcttatgttt ctggagagtc cttctttgca aataatggtc caaaaaatac tatgagactt
    33121 aactatacaa caatgtcaga agaacaaatc gttaaaggtg ttgagatact tgcagaagtt
    33181 ataaaaaaag aactcaatta tgcttgtgaa taaatataaa aagtgagggt ttaaatatgg
    33241 gagaaaaaga aaacggtata aagtttggaa agttaatgtt tcaacttgtt attatagcaa
    33301 tatcttggga attagtttat attataccat ttatccaata tacattatat gatccaattc
    33361 taaaagcatt agaatgtaca aacactcaac taggttttct acttacaatt tatgggttag
    33421 gtaatatatt tggagctcct ataggaggct ggttagcaga tagatttgat tatagaaaga
    33481 taattttagg ttctgtattt cttaatggag tagtatcatt tttatttgca tttaatatga
    33541 actatccatt tgcagttgct acttgggtag gttgtgcaat cacatcatta gttatgaact
    33601 atccttctat ggtaaaaata cttagagtta taggaaaaga taatcaaggt aaggtttatg
    33661 gatttaatga agctatggtt ggagtttctg gagtagtaat gggagcaata ttcttatata
    33721 tttatacatg ctttgcaact cctacattgg gtatgagatg ggtaatgata tctttaggaa
    33781 tattatcaat agcaatgtgt ccagtattat ggtttgtaat taaagatgta gatattaaag
    33841 aagaaagaga agaagcaggt gaaaaaatgt ctgctggtga ttttataaca gtactaaaat
    33901 caccaaatac ttggctagta ggaataagta tattctgtgt atattcattc acagttacaa
    33961 tgtcttattt tacaccatat ataacatctg tacttggagg ttctgtagca ttatctggtg
    34021 cattagcaat aataagacag catggtctaa aattatttgg agcaccattt ggaggatatt
    34081 gtgcagacaa attaaaatct cctacaaaag tacttttacc aatatatgta tttggtatag
    34141 cagtaatagt tttattccta gtattacctg catcaactcc aatgactata ttcatagctt
    34201 taacatttgt agtaggtata ttaggttaca tgggaaaagg tatatattat gcagtacaag
    34261 atgaagtaaa agttccagtt aaatattcag caactactat aggtatagct gcagctttag
    34321 gattttcacc agatgtattc caatttgcat taataggaca ctggatagat acttatggca
    34381 ataaaggtta cacatataca tttatattcc aaatagcaat tctagttatt ggaatactat
    34441 cttgtttata tatattaaag gttaaaaaga gaagagcaag taaattagaa gcttaaatat
    34501 tcaatttggt aattaagtat atataaatac aatacttatt ttgatgaatg tgattgtata
    34561 aataacataa aaaatatcaa tagattaagt tttataataa tgtttagaga ataaagtata
    34621 aaaatcaaaa tgttatcaag ttttctaggt tttaacataa agttacccct gaaggtgtaa
    34681 aattgagtgg caattattat ctattttctt acctacataa acctatttaa tttaaattct
    34741 aagagatttt aaaaatagat ggtagttgta ctcaatttta aacctcataa taaatacgaa
    34801 aatttaaaat ctaactagat actatttaat
//
LOCUS       DAMJWU010000033        34740 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMJWU010000033 DAMJWU010000000
VERSION     DAMJWU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.033"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC460_001757"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC460_001757"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF2600644.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTDPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="PC460_001758"
     CDS             complement(2851..3216)
                     /locus_tag="PC460_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2600645.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="PC460_001759"
     CDS             3415..4263
                     /locus_tag="PC460_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600646.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="PC460_001760"
     CDS             complement(4700..5788)
                     /locus_tag="PC460_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600647.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="PC460_001761"
     CDS             5913..6641
                     /locus_tag="PC460_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2600648.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="PC460_001762"
     CDS             complement(6843..7334)
                     /locus_tag="PC460_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600649.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="PC460_001763"
     CDS             complement(7345..7788)
                     /locus_tag="PC460_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600650.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="PC460_001764"
     CDS             complement(7778..8617)
                     /locus_tag="PC460_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600651.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="PC460_001765"
     CDS             complement(8836..10221)
                     /locus_tag="PC460_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2600652.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="PC460_001766"
     CDS             complement(10394..11137)
                     /locus_tag="PC460_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2600653.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="PC460_001767"
     CDS             complement(11157..12419)
                     /locus_tag="PC460_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2600654.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="PC460_001768"
     CDS             complement(12603..13601)
                     /locus_tag="PC460_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2600655.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="PC460_001769"
     CDS             complement(13585..15096)
                     /locus_tag="PC460_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2600656.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="PC460_001770"
     CDS             complement(15150..16112)
                     /locus_tag="PC460_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2600657.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC460_001771"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC460_001771"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2600658.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18105)
                     /locus_tag="PC460_001772"
     CDS             complement(17092..18105)
                     /locus_tag="PC460_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2600659.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18333..19310)
                     /gene="bioB"
                     /locus_tag="PC460_001773"
     CDS             complement(18333..19310)
                     /gene="bioB"
                     /locus_tag="PC460_001773"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2600660.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19516..19779)
                     /locus_tag="PC460_001774"
     CDS             complement(19516..19779)
                     /locus_tag="PC460_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600661.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19849..20922)
                     /locus_tag="PC460_001775"
     CDS             complement(19849..20922)
                     /locus_tag="PC460_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2600662.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21181..21939)
                     /locus_tag="PC460_001776"
     CDS             complement(21181..21939)
                     /locus_tag="PC460_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600663.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(21936..22853)
                     /locus_tag="PC460_001777"
     CDS             complement(21936..22853)
                     /locus_tag="PC460_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600664.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22853..23053)
                     /locus_tag="PC460_001778"
     CDS             complement(22853..23053)
                     /locus_tag="PC460_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2600665.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23058..24152)
                     /locus_tag="PC460_001779"
     CDS             complement(23058..24152)
                     /locus_tag="PC460_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600666.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24662..24802)
                     /locus_tag="PC460_001780"
     CDS             complement(24662..24802)
                     /locus_tag="PC460_001780"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2600667.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            complement(25112..26419)
                     /locus_tag="PC460_001781"
     CDS             complement(25112..26419)
                     /locus_tag="PC460_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2600668.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIVHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26484..26963)
                     /locus_tag="PC460_001782"
     CDS             complement(26484..26963)
                     /locus_tag="PC460_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2600669.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27010..27873)
                     /locus_tag="PC460_001783"
     CDS             complement(27010..27873)
                     /locus_tag="PC460_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2600670.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27873..28628)
                     /locus_tag="PC460_001784"
     CDS             complement(27873..28628)
                     /locus_tag="PC460_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2600671.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28655..29143)
                     /locus_tag="PC460_001785"
     CDS             complement(28655..29143)
                     /locus_tag="PC460_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2600672.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29161..29562)
                     /locus_tag="PC460_001786"
     CDS             complement(29161..29562)
                     /locus_tag="PC460_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2600673.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29565..30062)
                     /locus_tag="PC460_001787"
     CDS             complement(29565..30062)
                     /locus_tag="PC460_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2600674.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30086..30490)
                     /locus_tag="PC460_001788"
     CDS             complement(30086..30490)
                     /locus_tag="PC460_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2600675.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30720..33287)
                     /locus_tag="PC460_001789"
     CDS             complement(30720..33287)
                     /locus_tag="PC460_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2600676.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33453..34196
                     /locus_tag="PC460_001790"
     CDS             33453..34196
                     /locus_tag="PC460_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2600677.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT        11262 a         6360 c         3560 g        13558 t
ORIGIN      
        1 cttaaaaata attcgtcaat actttttaaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaacgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatacttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaaaaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggatctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcatgcc taacaatgtg cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggca tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctgtac aaggtatacc acaagctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtgttgg tgattcagaa
     4501 tagtatggta ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gcaaaaaaat
     6301 ataatgtaac cgattttcta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatgtttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagtttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaaaccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgatcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaat cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcatagttca aagaaaacct agagatttct ccatgatttc
     8521 tttttaagta tttacatagt ccacaatagt aagctttgta ataatcatat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttatgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttgttta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcctt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg tagctaacat ctccaatatc taatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg cacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg gtattcttat tggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcttct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta ccaagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttagta gtggatactt tttgagacaa
     9961 accatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccataact
    10021 agccacaaat gctgtatcca taccttctaa tccctctata ctaaaaccat gtttttttaa
    10081 acattctttt tgtaaatttg aagcatagta ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattatcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctacc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgttcag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccaaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaaccc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattac tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 ttttttccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gaccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacccgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatccacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattct cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaatat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcctcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcacc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattttgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatattt ttcaacatct tctttataag
    16201 gcataccaga ttgtgcacct agtttagtta cagacagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tgtttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattgcact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg tagccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgaa aaaaaaatta atatactttt ataattatct
    18301 aaaagtatat taattaattt aagcagtatt tattaaagtt caaatccttg ctctataatc
    18361 atttttttat cattttcaat cccacttcca acagttgtca aataatcacc aacaattgca
    18421 gaatttactc ccccattaaa tccaactttg tcatatccct ttaatgatag ccttccacca
    18481 gcatacctta tgtacacatt aggaattata aacctaaata ctgctgttgt ttttagtatt
    18541 tccattggtt ctaatacatc ataattttcc attggtgtat tcttaatagg atttaaaatg
    18601 tttattggaa atgattttac atttaattct cgtatttcaa aagccatttt tagtctctgt
    18661 tcttcactct cattcattcc aattataccg cctacacata catctagccc agctttttta
    18721 gcatctttta tagtttttat cctatcttta tatgtgtgtg ttgtacatat attatgaaaa
    18781 ttatctctac acgtctcaac attgtggtga tatgtagtca cacctgctga cttcaacatt
    18841 ttagcttgct cataatctat aatacctaaa gatgcacata attttaaatt agtattttcc
    18901 ctcaatcctt catagatatt taaaatatta ttaaactcct ttcctgacat acctttacca
    18961 cttgtaacca atgagaatct atgcactcct ttactctcca tctctttagc tctatttaag
    19021 atttcattgt aatctaataa tgaatattct tctacaccag ttttataata agaagactga
    19081 gcacaaaatt tacagtcttc actacatttg ccagatttag cattcattat cgtacataaa
    19141 tctgcttttc ttcccataaa atattctctt atttcatttg cagattttaa tagaatatca
    19201 aaatcgtttt tattatttgt atcaaggctt attaaattaa gagcttcttc ataacttatt
    19261 tctttttctc tcaatacttt attttttaat tttattatgt atttttccat atatcctcct
    19321 atttgtcaac ttaatatttt tatgtagttt acttattata tatagtattg catatataac
    19381 atttattggt caactttatt ttagacacaa gtttactata cacaaaaaaa tactgcttca
    19441 aaacaatatt agtatataga ttatacatat tattattaaa gcagtatttt ttattagaat
    19501 ataattctta ctggtttata aaactatcct tgtcttaact ccatccaagc tttccattgt
    19561 aataacttct aagccttcat attctctaaa tagccatttt aaatccttca tggcttgatt
    19621 aacctgactt tttgttagat tatttataaa atattctttt ctagtttcct catactcttt
    19681 gctagtcaaa aagtttctta gaaaattaaa cgcaaataca cttggaatat tgtttacatt
    19741 agccttcttg ctgttttcct tctcatagta aattttcata aatgcatact cctttgatac
    19801 ttttagattt ctcttacaat aattatatgc atttaagtgc tactattctt ataattttat
    19861 taattcatat tctttacttc ccatacccat ttcacatgca tagtctatta ttctagttcc
    19921 atttacatga ggatgttcat gtttgaaaac atcatgaccc atttccttac aaatcaaatc
    19981 atagcatgct tgttcaatag ctacagggtc tgttgatgct aatattccta tatcatgggc
    20041 aattggtcta cctgaccaag gtacacaatc acaaagtgga gttacattca taacaaaagt
    20101 tatataccct accttgtctt ttttatttga tactgctcca taagcgtatt ctgccatctt
    20161 ttcgacaaac acatctgagt cactttccca ttgtatagtt actgctctag ttggacagac
    20221 agttggacat tctccacatc cataacatac atcagaatct ataactgctt ttttatccac
    20281 tatacttata gcttttgtag gacaagaatt aacacacttt ccacaaccta cacacttctt
    20341 ttcctttact actggagtaa catcagaatg ttgcatttgt tttcctgctg cacttgcaca
    20401 tcccatagct aagtttttta atgctcctcc aaaacctgct gcttcatgac ctttaaaatg
    20461 actcattact atcattgcag aagaattata aatttctcca cctattttta cactttcaaa
    20521 atgttttttg tctattttta cattctcata atttctacta tataatccat ctgcaattat
    20581 tactggtgca tttacaactg cataagcaaa tccattttct atagcagttg taagatggtc
    20641 tacagaattt gttctactcc ctgtatacag tgtatttgta tctgttaaaa atggctttcc
    20701 ttcgcagtct ttaaccttat caaccacttg tctaactgcc acaggactca tatatgtagt
    20761 atttcccttt tctccaaagt gaagtttaat tgcgacttgg tcattttttt ctattaaatc
    20821 tttgaatcct gcttcatcaa ataatcttct tacattgttt ggtacgtttt tattttgcga
    20881 atttgagtat aaatcacaaa aatatacttt tgacttttcc atatctacac ccctttaaaa
    20941 tatttactta tactaatttt atcactttta aagtaatttt tagtatattt taacataatt
    21001 aaatattctt atatttattt gatacaaata atattatttg tattattttc tacacttaat
    21061 tctatatatt aaactacacc taaaaatcaa aaagacttga aacttaagtc caagtctttt
    21121 tgatttttag gtgtagtttt taacaacata agattacttt accttgtgat aaaggtatta
    21181 ttaaactggt tttttattaa ataaaataac agctgctagt aaacatacta tagaaataag
    21241 aactgttata ataattggaa taataaattc tggcattgcc actttccctg aaatcaagtc
    21301 tatatttttt gacatcaaga aattaggagt ataaggtgca atctgtttag gaatagaaat
    21361 taaactcaat aagagagttg ctaccaatac aagtaaaaca ctggcaaatg ctggtctaaa
    21421 tagtacacac ccaagaatta atacactaat atacataatt cctataagcc ataaattaaa
    21481 tgctgaaaat ataatatgat taagatttgc agttggccaa aaatacattg tatagccata
    21541 agcaaataaa aaactcaacc aaaaaccaat tgtcataata atcaccgtaa cagagaattt
    21601 agacaatact atagatgaac gtgataaacc tttagtcagc ataataacaa gtgtcccttt
    21661 agaatattca tttgataaaa tattacaaaa aataattaga gttaaactca taccaagacc
    21721 agagatattg ctaaaaaatt gtgtccaaga atctaaagca gttggtgcag cagtaacttt
    21781 taaagtcggt gcaaaatgag caatcaagtc tggcataaat tttgcagaaa taggactact
    21841 tataccaaat attagaaata aaataattaa actaaataat ttataatttc ttaaattctc
    21901 tttaaattct ttttttgtaa atgctatgta ttctctcact ttactacctc caaaaataaa
    21961 ttttcaagtg ataattctat tagttccatc tttactggac aaagattcat ttctgcgagc
    22021 gtagatataa ctgttttttg tattgctttt atatctttac catgcaaaac aatttccata
    22081 ttagtttctt ctgaatcttt tagtaaggat ttaataccat cactagactt aaacttttga
    22141 atttcatcat tacttgcaaa ctccagtaaa agtctatcct ttccgtgcat actttttatt
    22201 tctgaaagtg taccactaag ggcaatgctt cctttgttta atatagcaac atggtcacag
    22261 attctttcaa catctgatag tatatgtgtt gaaaatatta cagttgttga gtctttgatt
    22321 ttaagcataa tatctaatat ttcttttcga ccaacagggt caagtgcact tgttggttca
    22381 tcacaaataa gtaatttggg gcgtgaaagc aaagcttgag ctataccaag tcgttgcttc
    22441 attccacgag aaaacccacc tattcgtctc ttttcatttc taagacccac aagtgataac
    22501 aactcttcac tccttatctg tatttctttt tttgaaagtc ccgtaatttc accacaaaga
    22561 gaaagatatt caagtggtct catataattg taaaactctg gaacatcagg cagataacct
    22621 acatgacgat tagaacttgt tttcccatag gtcactttct caccacaaac atctatagtt
    22681 ccactatctg gtttaagtaa cccaagtacc attttcattg ttgtagtctt acctgcacca
    22741 ttcttaccta caaacccaaa gactgaccct tctggtacag taaaatttaa attatctatt
    22801 atctttcgat taccaaaacc ttttgataat ccatttattg agacaatatt catcattctt
    22861 cacctcttcc tattgtaaaa tacaaaattg gtcctattaa actaataaac agtactacaa
    22921 taatccatat tgccttattt ccaattttat aatttggatg tctcaacaca tgaacaagtg
    22981 cagtaattat aagtattaaa tctaaaataa taacaggaac caaaagtggt aagtattcca
    23041 tcaaattttc cataatctta ttcctccaat aaagataatt tttctataag aaatttgtat
    23101 tctttgtttt cacttaatac agaaaacatt ggattatttt taatagcagc aacaatacct
    23161 gcttttacga ttgtatcatc acgtgtaata cctgttccaa tttcagaaat ccatctgtca
    23221 agtttattaa aaaagtcatc cccatgtatt ataattgggt atatatcacg tgttgctaaa
    23281 tctacatatt ttcgtaatga tttaatagct ccttcattat caccatgtat taaattaatt
    23341 tgtgcctgtg ttaaatatga agaaagcata atagcaggag ataatgtatc aagctcaaat
    23401 atatctgaaa ttgctagaat acgtctttct atttccttca tttgtaattt atctgcattt
    23461 tgcaaaatac ccactagatt ttggactaat gatataaggt tctgatatgc accaagttga
    23521 aatgtttctc ttgcttcatc catttgacca ttcatcattt gcccttgtgc aagaagtata
    23581 gattcattta tcatgggaaa attgctgttt tgcaataaat caatcacttg tatgggttga
    23641 tttaacaata tataacaagt tgcctccata ctttttgctt gacggcaaat atctatgtca
    23701 ttacaagttt cctgtatacg aataaatatt tctaatgctt cactaattaa tagttcacgc
    23761 tttttttcat ctacaatatc atagtgatta actattaaaa gacccatccg aaaaatcaaa
    23821 ggaaaacatg aataatattt ttttatgatt tctcttattt catccatgac ttcatcaaat
    23881 ggtttaaccg taaagtcttt gcacagttta ttatacagtt tattaatatc ttccttcatc
    23941 atttgaggtt cataacatat taactcatca acggtaatgt tgaaatatgt cgcgagttgc
    24001 ggtaaaaagg ttatgtcagg atagctttgt ccagtttccc atttagatac agaagcttta
    24061 gaaacgccta tataatttgc aagttcatct tgtgtaattc ctttttcttt acgctttaaa
    24121 atcagcgttt ttgcgatgtt taattcattc atgtcaatcc ctccttatgt ataattatat
    24181 ccttaaacat gtaaagctaa caagcgattt gaagttgact tttgacgaaa aaatcaacca
    24241 cagagaaaca aataaacaag tatataaaat atatatttaa atttatcttt aatctccttt
    24301 gtaaaatatg taaactttgt aaataaaatc catattatat aaaactaata gttaatctat
    24361 ctaaatacag tttttattta tcacgttttt aaatattttg tgtaaaacct cttttaatat
    24421 aaacacctta ttttcatttc tcatttcata ttctttttaa aatactgaat ataactcttc
    24481 gattttccat ttaatccttt tataaagtta ttattcgtat ttattcctct aattataaca
    24541 ggcaactatc atgtattggt atttgtctat tatataaaaa tctataaaag aaaagcagtc
    24601 aatcatttag attaactgct tgtacatata tcttattaaa ctgtataaac tataattttt
    24661 attattctcc attttcatcc caagtcataa atccccatcc actatctgga taaatatcga
    24721 tagacttttc tctaatcata taaccaactt tataacactc ttttgttatt ttattagata
    24781 gtatacatcc ttctccattt atctaattcc atttaattaa cttttctttt caaccttaac
    24841 ttttataaaa tcatcttttt taataatttc atacttattt tctcttcaac atcatcaaat
    24901 gagggtgtct caaaattaac tttttacttc ataaggcact cttttttgta tgaagtaaaa
    24961 tatattttca tcatttcaac aataagtaag aggcttatct ctattttgag acagccccat
    25021 ttgatggtgt tgaacattta aataaggttg aactcgtttt atctctcttt ttaagatatc
    25081 tgtaaatttg cacataaagt ctcttcttct tttacaattc taatagtttt attaaagcca
    25141 aagtatatat ctccatattc tttcgtatat gatttattgg tactctttca tctactccat
    25201 gaccaccagt tttatacgct aagaacttag gtccaaacgc caccatatct ttaaaccaac
    25261 ttgcatatgt gccaccaccc atagcaattg tatatccatt ttcatctgtt acttcttcat
    25321 aggcttctaa taatttttta agtactaaat tactctcatc cacataatgt aagtctttat
    25381 gaacaataac ctctattttc caaccattac aaaaaacctt tttgaatcta tctattatat
    25441 tgtttaaaac tatattttta ccatacctta tatctaactc acatacgatt ttttcctcat
    25501 ttacttttaa tattcctaaa tttaaggtag tgtttttgaa tacctcatca tattgattaa
    25561 tccccaatgc atctccataa taatcactac aaaggtattt tgaaactaat tctatatctt
    25621 ctctatgctg aaaatccata ttatcctcaa ctatctttat taccatttta gatatagcat
    25681 tttctccctt ttcaggatta cttgcatgta cagctactcc tttagtagtt aactctgaaa
    25741 taattccatt aataattttt gcagtacaat gagatggaac aacattttct ttagttcctc
    25801 cacttactat tatgtaaggt ttacttttgt caattttaat ttctttttct atccttatat
    25861 gaacccttcc tttttctcca ttaaccactg gatacatagc atctggagta aatcccatac
    25921 ttggaatttc ttcaccttta tctaagtaat atttgataca tttcatacct gtctcctcat
    25981 ttgtaccaaa aattaatctt attcttttat taattgtagg atacatatcc tctatgtttt
    26041 tcaatacatg tataagcata gatacagcac ccttattatc tatgacacct cttccataaa
    26101 taatattgtc aattactgct cccgaaaatg ggtctacact ccacccctct cctggtggaa
    26161 ctatatctaa atgcattgga atccctatta attcctctcc ctctccaatt tctgcatatc
    26221 caatataatt atcaatattt ttaacactaa aacctaaact agcacagtaa tctaaagttt
    26281 tatctaaaga ttttttaggt cctataccaa aaggacattg tgaaataggt tcaccttgtt
    26341 gtgaatcaat ttttatcaat tcttttagaa attctatggt tctatttaaa ctattatcta
    26401 cacttctgct tatatccata aatcaaccta ctttctatct ttttaaagcc tgtccttaaa
    26461 gaacaggctt ttagcattaa aaattatttt tttgtaaata ctaattcaca aacttcatca
    26521 tcatgagcac atttttgagc aaaatctaat ttaacacctt gtgcacagtc aactcttcca
    26581 taatccccat aacaagctaa tctacaaatt tcttttcttt cttctttagt taatccatat
    26641 tctttccaag cagcatctaa tggacagtga tggaatttta aaacgccatt ctcatctgta
    26701 ttttctacta attccatttc aaacactttt tgtcctttac tgttatataa catttctgcc
    26761 atttttcctg gagtatctgc tacgttatat ttttgacctc tcattttacc aaatccatat
    26821 attgcttctt tacatatatc atctggatta gctcctaatt tttcagcttc tttcataaga
    26881 agatagaacc atactgctct ttctcctgtt gctcctctaa ttccttctaa atattccttt
    26941 tctgtataca ttttttctga catttctttc tcctcctaaa attaaattct atattttaat
    27001 cactgtgaat tagaatattc caatgaatga ccctaccaat gctaatacaa ccaaaattac
    27061 taataactta gttattgtcc acttcttgta tttcattccc caatatacga agaatacaac
    27121 accaagtggt aataaacctg gagcaatcat atttaatgta tcttgaacaa caattgcttt
    27181 accaccaata tcaaatgata gcggagttga aagcttaact gtagttgcag ataatgctcc
    27241 catcataaac attcctagaa ttgaagcacc atcaattatc ttattgacaa taccacctga
    27301 tagaattttc ttaattgaat ctctacctaa agaatatcct aaatgaaata agtaataacc
    27361 ttcgcaaaat agtaatatac taaacattat tgggaaaaat gcacctataa cacttccttc
    27421 tgctccaaat gaagcaccta aagctaagaa tattgtttga aatgtaccaa aatctaaagt
    27481 atctccaatt ccagccaaag gtcccataag accattctta acacctgaaa taacttctcc
    27541 tgggattttt ccttcagtag ctttctcttc ttccatagct aaaactgtac cgtgaattaa
    27601 tcctccccat atagcttgag tattaaaaaa ttgtaagtgt ctctttaacg catctactaa
    27661 atcttctttt ctcttatata acttttctaa agcaggagtg aaacttgcac aaactgctaa
    27721 tgcttgcatt ctttcaaaag agttggataa ttctgctgtc caccaccatc ttagatatgt
    27781 ttttgttaca tctttctttg taagtactct tgtcttatta acttcagttg gaccttgtcc
    27841 atttttaact tctttatttt ttatttcttg cattatactt cacctaccct tttgtcttct
    27901 ttcataaagg taattaaaag agctatacaa acaccaaata ttgcagctgc agtaattgat
    27961 atttcaaaat attgaaccat aaaaaatcct ataataaaca ttggtaagaa tttattttta
    28021 cctatagtaa atattgttgt tgcaaaacct aatgcaggta atataccgcc cattactgtt
    28081 aatccattca ttaaccattg tggtataaca tctaataatg attgaactaa atctgctcca
    28141 aaaaagttac atacaaaaac tattggaaac cttaaaacaa acccaaatat taaagaataa
    28201 agtgttgcac atctccagat gccttttgta ttaccttctt cagcatactt atctgcctta
    28261 tgaaccaata cagcattacc agttcttctt aagttattaa ctaatactcc tataacacct
    28321 aaaggtaccg caatagatac tgcaacttct gcattaacac cagtttgaag tgctactggt
    28381 attgcaataa gagcagctag acatttatct gatggaatat ttccccctgc tgcgaccatt
    28441 cctagataaa ccatttctat acttgcacca acaatcatac cagttgttac atcaccccaa
    28501 agtaatccaa ggctaaaacc tataaataaa ggagacttta gtgttgagaa aagggtatac
    28561 cctgcaagac ctgctgcaaa ccaataccat aatcctgtta caattgccaa catcaaaaca
    28621 gattccattt ataattcctc ctaatttata ttttttattt aaatttcttt aacgcatttt
    28681 catatgtcat agatgattcc tctggtataa cttgtaggac tatttcaacc ccattttcat
    28741 taagttcatt aagttgttct acatcctcat ttcctaatga aacagctgta aacaccattt
    28801 tctttcctgc ttcatttgga atacctccta attgaatctt ttcatattta agtccagctt
    28861 tataagcctt atttgccata tcaacatttt taaatagaat aaatatattt ttatctccta
    28921 aagtgttgtt ttggaatgct tgtcccgcat cttctgctgt taaaataact acttctactc
    28981 ctgatggtgc tgccatttta tatatgtcag ccataaattc atcttgacct aagaagtcat
    29041 caactattat tattttttga gcttgagcga tttttaccca ttttgttata acttgcccat
    29101 gaattaatct gtggtctact cttactaatg ttatttttgc cattatattc acctcataat
    29161 ttatattttt ttaaattctt taattaaatc cacacaaagg ttattatttt ttttgacgat
    29221 tctttctggt aatgtatgta atttatcttc tttgcttaat tcttcatcta cgattaaaat
    29281 tgtctgtaag ctaagtccat ttattgcatc tattcctctt cttatagcaa cagctattgc
    29341 aacacttgat gtagatcctc catataaatc tgatattata attttcacat cttctttatc
    29401 tactacttct tcaacctctt tcataaatac agaaacatct ttttcagaac ttagtgataa
    29461 aacttcatat tgatttgaca atccaaagtt tttatttgtt tcttctacta agacttttcc
    29521 ccaagttcca tggcacagta tgattacctt atatttattc attgctaaat aaactctcct
    29581 ttatagcgat taatattttg gtttaattct acttcttctt catcaggtac catttgaatt
    29641 gatacattaa tattcttttt aataaattca tctattaaaa gaaactcttt tttattaatg
    29701 tatacatttt gagatatttt cttttttcct attcctgtag gtattcttcc aacaataagt
    29761 tttttgatat taatcccttc ttctatcatt ctatttgcag tatctaaatt tttaattaat
    29821 acaaaaacgt tatcatcagc ttgattatta taaaaatatc tccttgattc acttacagtt
    29881 aatattgata aatcaatatt ctttggtgct gccattttta tcaaaccttt agcaataaca
    29941 tcattggttg tttcatcatc tactactact atctttttta cacctagatg cttaacccat
    30001 ttatatataa cttgaccatg tattaatcta tcatcacatc tgcaatattt tactaaagac
    30061 atttttaatc aaactccttt ttctattaca ttgagctaag aactttatta atattgttta
    30121 taccagtttg tcctgcttca atacatgctt ctgctaactc ttctaaactt tttccctctc
    30181 tattaattaa agcttcaagt agcattggaa gatttactcc agtaagtgca tgaaacttgc
    30241 cacctttttt aaggtttaaa cttgcaacat tacttggcga acctcctaat aggtcagcta
    30301 atactaaaac tccgtcacct tcatctaatt catttaactt ttctacaaaa tttttattta
    30361 agtcttcaat attatcattc ctatttagtt taattgctgc tgttctttct gctggtcctg
    30421 caataagttc acagcttttt ataatttctt cactaaaact cccatgagtc ataactatta
    30481 ttcctatcac caatttcacc tcacttttct tgtcactttt tatataagca attgccgtgc
    30541 caacttatgt gtatcaataa caaatggcta ttttacagga tttaatcaaa actttttact
    30601 caattttttt taaaaactta ttatttcgat atgtatcaaa agattttttc aacctgtatc
    30661 aaatctgtat caaaaaaagt aaaaaaaaga tagccttttg gctattttta aattttgtat
    30721 caacttttga atttatctgt atcatattgt gattcaaata tatctaatat atttgcatat
    30781 tccatatctg aaataattat atcgaatgtc tcttctacta gcttaaaact ttcttttata
    30841 actaaatata attctttatt tctactaatt ctttcttcat atgatttata aactaaagac
    30901 tcttttatta ttactctttc aatcatacat gaacaatgaa atataaactt tactaaaagt
    30961 tcatcttgaa ttactaaaga atagtgttca ctaattctat taattacttc ttttaatata
    31021 aaataagcct tttccccatt taaaaaagtc aaagttttac taatattatc aattagtaat
    31081 ctatcaaaat atctaccacc attatctttt tcagcatatg taacttccat atctccataa
    31141 tgtttcttta tagattctat aatattaaaa tataatttct ctaatgtcat ttctggagtt
    31201 agagcctttc tagttgcttc tattacaata ggtgtactta ccatatctat tgcttttact
    31261 tcttttcctg tagcatcacg aataacttta gcaaatgtta aaatagaccc catatcagtt
    31321 aaaattaaaa ttccctttcc tttatcaatt ttattggcta tttctacagc ttgtactaaa
    31381 atatcctgaa ctctatcttc taatcccata ttaattgcca ctgcatggtt acaatccagt
    31441 aaagtattgg ctacattcac catgctagat gctgtagatt caccatgcat tatgactaat
    31501 attgctatac tctcctcatt taatcccaca tacgataaat aaagaaatgt tgctaaaaaa
    31561 attgcttcat cttctggaaa ctctactcca tacattaatg acatgttaga taatatttct
    31621 tttgctatgt tatattcata tgctctttcc ttcataattt catctttatt aggatgcttt
    31681 actatatttc caatctttat tctttcaatt aaattctgaa tatgtaaagc tatagctttt
    31741 attaatttat ctaatccttc tcttttgctc catttatcat gcttttgtaa aatatatttt
    31801 acagtagttg caattgaatc attaacaatg ttgttataag ctgtttgatt actactatta
    31861 tatataaaag tattcattaa attataggaa tacttctgta tgtctccatc tataacactt
    31921 ctaatttgtg actctttaat accttcttgt tgaagtttat tccaagtttc ttttatcatt
    31981 ccataaaaat ctaaatcata ttttttattt tctattaata aattttcact ttcaaaatcc
    32041 ttttcttctg gtaaaaaaat aatatcatct tttccaataa aactgaaatt ttgtacaaac
    32101 tcctcttttc tattatttcc atataatgat tctctaatat tatttggtag aagagagagc
    32161 cttacatata cacattccct tttataggtc atataatcta aaaaagctct tgcacaaatt
    32221 aattgtatat cgctttttaa ttggcctatg tttcccttac acttatataa aataaatgcc
    32281 ttaataactt cttttgaaac ctttaactta acaccaatcc taacactctc ttctttaaaa
    32341 aaataacata taagcttcat tctctcttga aagcctcttt cctctaaaga cggaatcttt
    32401 atagttacag gtattcttct taaaaacgtt tccaacattg ctgtttctgg gtcttctgta
    32461 gtggctgcaa ttatcataac tctacactcg tgtaccttat tagcctctcc taaccttcta
    32521 tatatccctt tatccataag cagaaatagc atttcttgtc cctctgcaga taatctatga
    32581 acttcatcta aaaacaatac tccattatta gcttcatgta caataccata cttatcttta
    32641 tcagctccag taaatgctcc ttttacatat ccaaaaagct gcgctaataa taattgctga
    32701 ttatcagaat aatcagcaca gttaaatatg ataaatggtg cattatcact atcttctttt
    32761 atagttaaac tatacttata catatattct gcaaatatag atttaccaac tcctgtaggt
    32821 cctattatta atgtactcaa ccccttaggt ggatatatta ctgctgcttt agcctgttct
    32881 atttgggacc ttaaacttcc atcacatcct actaaattaa aaatattgtt ctcttttttt
    32941 atactctcta aaatatactc atctttgata gaatcatttt cactatattc gtttatagag
    33001 ttaatattat caaatatccc tttatttagt ttctttccta aaatactctc aatactttct
    33061 ttatataagt actgaacagg cttacccttt atctttataa taatactttc ttttattaat
    33121 tgatttaatt ccttactagc attacttcta tttataccta cttcagtagc tatatattct
    33181 gcatcaaatc caaatctaat gccattatta aagttcaagt gttcttctgt taaccttttc
    33241 aagcaaatta aaactttttc tttccttgat agcttctttt gtgtcatagt taacctcccc
    33301 tataagcagt aatactgtta aacatttatt attaagctta attctacaat attttaatat
    33361 ttttgtctaa ttatgtaact attgattttc tttcctattc gttttataat ataaattacg
    33421 ttaccctcat cttttataga ggaggaattt atatgaattt tttatcagat ttacacaccc
    33481 actctatagt cagtggtcat ggctatagca ctttactaga aaatattaat tattgtaaag
    33541 aaaatggaat taagatacta ggtacatctg aacatggtcc taagatgcct ggttctcccc
    33601 acagatggta ctttcacaac attcaaaata tcccaagaat cataaataac attataattt
    33661 taagaggctg tgaagcaaat atactagata ttaaaggaaa catagaccta gagccttttg
    33721 taattcctcg cttagattat cttattttgt catttcacga agctgtgttt tctccaaata
    33781 cattagagaa taatactaaa gctttaataa atgcaataaa taagcatgat aatattgaaa
    33841 tattaggaca ccttggaaac ccaaactacc caatcgacta tgaattaatc attaaattag
    33901 ctatagaaaa gaatatctta atagaaataa ataattgttc tattaaaggc gtgtcaagaa
    33961 atggtagctc ggataactgt aagtatattg ctacactttg caaaaaatat ggtgctaaaa
    34021 tcatactgac ttcagatgct cacatttgtt ttgacatagg taactatgag tattctgaaa
    34081 atatcttaaa ggaaattaat tttccagatg aattaataat gaattatcct aaaaaactta
    34141 ttaatcattt tcaccaaaaa ggtaaattac tagatgttga ttatacaaat atctaagtaa
    34201 taatacatgc ttactttaac tcccataaaa attttaatac tatttatatt atatgaaata
    34261 aagaaattat aataaagaag ctatttaata aagcttaatt acttttatga acttgatgga
    34321 ttgatatggt ctatgatagc aatagaaatt ataacatttt taataaactt agttttatat
    34381 accttatagt ataaatctaa aaaagcatca tctaaaaagg agctgtcaca tgagcaaatt
    34441 aaatttgttc tatgatagct ctttttttac ttattttaat taggtatatt tttaattatt
    34501 ttatataaag cttatgttct aagtaaaatt atgcataaca aacaaaggta taaaaatacc
    34561 attttttcag ataatattta attataaata ataaatttag aatacttctt ttaaatgatg
    34621 agagtctaga aatttttatg tgtattcttt aaataaaaat ttccagactc tagccccttt
    34681 ttgaatattt taaaatttaa cttaaactaa gcaaattcta taatagctta tctctaaata
//
LOCUS       DAMJWU010000034        33508 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMJWU010000034 DAMJWU010000000
VERSION     DAMJWU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.034"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..1119
                     /locus_tag="PC460_001791"
     CDS             <1..1119
                     /locus_tag="PC460_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2600678.1"
                     /translation="FLFVKYIIKQSTVLSIVSTLLVYLIEQLVYGFTAPLNYLIFTSD
                     INTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNIKVMNFDKYIAILMIPLLLIILF
                     MQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQAL
                     EDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQD
                     YLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDL
                     CIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGL
                     LNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            1158..1643
                     /locus_tag="PC460_001792"
     CDS             1158..1643
                     /locus_tag="PC460_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600679.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(1906..2142)
                     /locus_tag="PC460_001793"
     CDS             complement(1906..2142)
                     /locus_tag="PC460_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600680.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(2297..2521)
                     /locus_tag="PC460_001794"
     CDS             complement(2297..2521)
                     /locus_tag="PC460_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2600681.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2618..3871)
                     /locus_tag="PC460_001795"
     CDS             complement(2618..3871)
                     /locus_tag="PC460_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF2600682.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            4131..5120
                     /locus_tag="PC460_001796"
     CDS             4131..5120
                     /locus_tag="PC460_001796"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF2600683.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5192..5932
                     /locus_tag="PC460_001797"
     CDS             5192..5932
                     /locus_tag="PC460_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF2600684.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            6148..7299
                     /locus_tag="PC460_001798"
     CDS             6148..7299
                     /locus_tag="PC460_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2600685.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            7324..8079
                     /locus_tag="PC460_001799"
     CDS             7324..8079
                     /locus_tag="PC460_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF2600686.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8271..10658
                     /locus_tag="PC460_001800"
     CDS             8271..10658
                     /locus_tag="PC460_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF2600687.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(10889..11122)
                     /locus_tag="PC460_001801"
     CDS             complement(10889..11122)
                     /locus_tag="PC460_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2600688.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            11411..12001
                     /locus_tag="PC460_001802"
     CDS             11411..12001
                     /locus_tag="PC460_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2600689.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            12115..12960
                     /locus_tag="PC460_001803"
     CDS             12115..12960
                     /locus_tag="PC460_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600690.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            12957..13640
                     /locus_tag="PC460_001804"
     CDS             12957..13640
                     /locus_tag="PC460_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600691.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            13637..14353
                     /locus_tag="PC460_001805"
     CDS             13637..14353
                     /locus_tag="PC460_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600692.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            14450..14713
                     /locus_tag="PC460_001806"
     CDS             14450..14713
                     /locus_tag="PC460_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600693.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            14685..15140
                     /gene="def"
                     /locus_tag="PC460_001807"
     CDS             14685..15140
                     /gene="def"
                     /locus_tag="PC460_001807"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2600694.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            15192..15653
                     /locus_tag="PC460_001808"
     CDS             15192..15653
                     /locus_tag="PC460_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF2600695.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            15703..16605
                     /locus_tag="PC460_001809"
     CDS             15703..16605
                     /locus_tag="PC460_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2600696.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            16975..17787
                     /locus_tag="PC460_001810"
     CDS             16975..17787
                     /locus_tag="PC460_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2600697.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            18835..19596
                     /locus_tag="PC460_001811"
     CDS             18835..19596
                     /locus_tag="PC460_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2600698.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            19661..20443
                     /locus_tag="PC460_001812"
     CDS             19661..20443
                     /locus_tag="PC460_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2600699.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            20710..21129
                     /locus_tag="PC460_001813"
     CDS             20710..21129
                     /locus_tag="PC460_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF2600700.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(21232..21498)
                     /locus_tag="PC460_001814"
     CDS             complement(21232..21498)
                     /locus_tag="PC460_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF2600701.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            21725..22426
                     /locus_tag="PC460_001815"
     CDS             21725..22426
                     /locus_tag="PC460_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600702.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            22508..23116
                     /locus_tag="PC460_001816"
     CDS             22508..23116
                     /locus_tag="PC460_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2600703.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            23440..23904
                     /locus_tag="PC460_001817"
     CDS             23440..23904
                     /locus_tag="PC460_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HDF2600704.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            24541..25557
                     /gene="gap"
                     /locus_tag="PC460_001818"
     CDS             24541..25557
                     /gene="gap"
                     /locus_tag="PC460_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2600705.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            25952..27121
                     /locus_tag="PC460_001819"
     CDS             25952..27121
                     /locus_tag="PC460_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600706.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            27501..31754
                     /locus_tag="PC460_001820"
     CDS             27501..31754
                     /locus_tag="PC460_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2600707.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            31903..32397
                     /locus_tag="PC460_001821"
     CDS             31903..32397
                     /locus_tag="PC460_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2600708.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            32513..32890
                     /locus_tag="PC460_001822"
     CDS             32513..32890
                     /locus_tag="PC460_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF2600709.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            33096..>33508
                     /locus_tag="PC460_001823"
     CDS             33096..>33508
                     /locus_tag="PC460_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF2600710.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKD"
BASE COUNT        13507 a         3397 c         5610 g        10994 t
ORIGIN      
        1 tttttatttg taaagtacat aataaaacaa tctactgttt tatctatagt atcaactttg
       61 ttagtatatt taattgaaca attagtatat ggatttacag ctccattaaa ttacttgata
      121 tttactagtg atataaatac agatatttat aaatttgata taaatatggg actatcttct
      181 ttaattacta ttataatagc tggatttata tattggtata gttctaaaaa gtttaatatt
      241 aaggtcatga actttgataa atatatagca attttgatga ttccattgtt gcttataata
      301 ttatttatgc aatcatttga atatagttca aatataaata tagatacaag tcttgggatt
      361 gtaaagctgc tattaaatac ttctataact gaagaaattc aagcttttct tttttctata
      421 attggaacta tatgtgtatt tttttctcta tttaccttta agaaactaat tcaagctctc
      481 gaagatgaca aggagagggc aattatgaat caacagattc atgctcaaaa aaactatatt
      541 gaagaagcga aatcacgatt atcacaaact atatccttta gacatgattt taataatcat
      601 ttagctattg tcaatggatt gttgaaaaaa gaccaaattt taaaagcaca agattattta
      661 aataagcttg aaaaagtaac aagtgaccta tcattttctt gtaatacaca gaatattgtt
      721 attgatgctc ttttcaacaa taaattaagt atagcaaaac aattaggcat agaggtagat
      781 tgtgaagtaa ttatacccca aagcactaac atgaacgatt ttgacctatg tatagtgttt
      841 gctaatgcta ttgacaatgc aattaaagct tgtagagttg tagataaaca aaataggtat
      901 ttaaagcttt ccactaagtt agagggtgga ttttttatga tagaaataaa aaatagctat
      961 aatccaataa ataattatac aagtggctca ggtataggtc ttttaaatat aaaagaaatt
     1021 gctaaaaaat atcaaggggc tataagtata gataagacat cagattattt tcaaataaat
     1081 gtattgctgg ttatttcatt acattgcgct agtatttgat aacaatttca ttgaaaatag
     1141 tatttgtaag ctatattatg tacttgaata gagaaagttt gatatataga aatgaggagg
     1201 ttactaaaat gaaagttgga aatataaatg gtcaagcacc aattacaaat ggtttagata
     1261 gttgtcaaag tgaggtacaa ggcttagaac aaaagaaaaa atcattacag gatgaaatag
     1321 agaaagtaag aagtgataaa agtattgatg gtgaaacagc caaattaaaa atagaagctt
     1381 taaaaaaaga aatggaagag attgaaaata aaattcaaga gttaaaagga aataaagcaa
     1441 aaaaaacaaa taaagaagaa aataatgttt cagaagtaaa taataatcta ttagagagaa
     1501 aaaaagaacc ttctaataat gaaaatgtaa aacaattata tgatgttttt gaaagaagca
     1561 atgtagacga acaatgggat aatacttatc gtgttctcca agatgataaa aacttaaaag
     1621 taaaatttaa tagacctata tagtttattt ataaaaatag ttaatatttt aattatcacc
     1681 actttttaga agaatacaca ctttatttta aagacccaca tatttattta cagttagcac
     1741 atttatatgt ggttttataa ctattattta caagtctata ttgataactt ttacatagta
     1801 tataatgttt aaatacttat tcaaatttat ctatacataa acaaaaaaag agattttaaa
     1861 atctcttttt agattgttga cttatggctt tttgatttaa taaaatcaag aagccgtttg
     1921 aattttatct gttggctgat acccttctga tattgaaaca ccatttttgt ttccaaaatc
     1981 gtttaaaatt aagaacttat tatgaatgac tatttcatta tgaacaataa gcagataaca
     2041 tgaatcttca atcaatgttt taacagtagc attaaatgct tgcttataca gttctatcgt
     2101 ttgtttgaac tgtccattaa agtaaatatg agtcaataac ataactatat ctacacaaat
     2161 taatataaat tttttatact gtacattatc taactgttga ttatctgtat tacaacatga
     2221 ttgaatttaa aaagacatca acacatagaa tgttgatggc ctgaaaatag atttaaaatc
     2281 tcttttttat tatatattaa ttatttttta aatttatatt ttttgcatca gattttctaa
     2341 tagctaagtc aaaccctcta aatctaatca ctatagggtc attaagagga gctatctttt
     2401 tcacttcaac ttcagtgcca tctattagtc ctaaggctag aagtctcttc ataagttttt
     2461 catttccagc tatattgtct ataattccct tttgtccaag ttttaagttg taaacagtca
     2521 ttattaacac ctcctaatga aaatcattat caaactttat atttataata tataacaaaa
     2581 agagcaaatt gtcaatatat aacaaaaaat aaacatacta agttagtatg tttataaaaa
     2641 atagtataca tgggatacta atagcatcta gcaaaaaacc acttactaat ggtacaacta
     2701 aaaaggcttt tggagaatca ccatattttt gtgtcaaaga actcatattt gccagagcgt
     2761 tcggagtagc tcctattcca tgtccaatca aaccacttat tatgatggca gaatcaaaat
     2821 cttttccaag tactctaaag caaataaata ttgaaaatag gattataaag attacttgaa
     2881 aagttactat taagagaata ggaggtaata atgtagataa ttcaaataaa tctatattca
     2941 taagtgataa agttaaaaaa attcccaatg aaagttcttt taaaaaatct ataagtttaa
     3001 aatttaattc tagtatattt atcttatcat ttaaatttct aaaaataaca gccgaaaaca
     3061 tacaagttac aacagagggt aatagtatat taaacgttat atagaagcat ctacttataa
     3121 tttctcctgt atttatacaa ataaatatga gaagaacctg ttccaaaaat acagatggtg
     3181 ttattgtttc ggagaagtta ttatttttta tcctattctt atttctaaaa aatttacttc
     3241 caaatctctt tagatttcta ttatatctag gaatcgacaa gtctatattt gatgatggtt
     3301 ttaatttata tttatttatt agaaatttag ctacaggaca tcccaatata ccacccataa
     3361 taagtcctat tgttgcagca gataatccaa ccccaactgc tccttctata ccgagatttt
     3421 ctattgtaag tccaaatgca gcagaataac catgaccacc ttccatagat acatttccac
     3481 acataagccc taaaagaggt tcaagtttta taatctttga tattgtaata gctaatatat
     3541 tttgacaaaa tgccaatact ccacacaaaa tccaatatct aaataataca ctaccacctt
     3601 tctttataag atttatgctt atttcaagtc ctatagttgt aaaaaagaag gttatgaaat
     3661 ttggcattaa atttccactt atagatatat ctgctatatt aaaatattta aaaaataaat
     3721 ttagaaaaca aaataataga cctcctatta ctggtgcagg tatacagaaa ttatttaaaa
     3781 agttaacttt attcttcaat aaattaccaa ttaaaaagaa gaatatagat agtgtcaagg
     3841 tttgcactat atcaaaactt aaggtaatca aaataagtcc tcctttgtaa ttcaaagttt
     3901 taattatata acttaaattc ttatgtattg aaattttatt atcaaatgta tattaataaa
     3961 tgtatattaa aaagaatcag tagatataat tagatagtca taaattaaat taaaatattt
     4021 taatgagata tgactttttt agaattaaaa accgtagtat attgatataa tagaaataag
     4081 acttaaaaat ctgttgatag tattatttat aagtacagta ggaggataaa atgtttgaaa
     4141 atttacctaa gatagattta cattgccact tagatggaag tgttagagta gaaaccatgc
     4201 ttgatatagc tattaaagag aaaatagatt taccttcaaa taatatggat gaaataaaaa
     4261 aattagcaaa ggtttctttt aattgtactt cactagatga atatttagaa aaatttgatt
     4321 tacccttaaa agtaatgcaa tctaaggaaa atttaaaaag aattactttt gaacttttgg
     4381 aagatgcaag tagagaaaat gtaaaatata ttgaaattag atttgcacca ttacttcaca
     4441 cccaaaaagg tatgagtgtt aaaaatataa ttgagggtat aattgaagga attagagaag
     4501 ctgaaagtat ttatgatata aaaggcaatt taatattagg ttgtatgaga acaatgacct
     4561 caaaagaggc attattagta attgaagaag ggaaatcatt tgtaaataaa ggtgttgtag
     4621 cagtagactt gtgtggtcca gagaaagagg gattttgtaa ggaatataaa gatgttttta
     4681 aattagcaag agaatatgga tataaagtta caatacatgc aggagaagct gcaagtggag
     4741 aaaatgtatt ggatgctata aatattttaa aggcggatag gattggtcat ggggtaaaaa
     4801 ttaaagacca taaaaaggcg tataatttag ttaaagataa aaaaatattg cttgaattat
     4861 gtccaactag taatgtacaa actaagacag tagattctta tgaagcacat ccattttata
     4921 ctttttataa agataattta catgtaagta taaatactga taatagaact gtatcagata
     4981 taaatttaag ttcagaacta aatgttatat ttgatacttt taaactagga ttagaagact
     5041 ataaaataat ttatagaaat gcagtagaag ctagttttgc agataaagaa actaaagagt
     5101 atttgaatag tttaatttaa tagataaaaa tatttaaact ttaaattaag ctattaaatt
     5161 gataaaaata cattgtatta ggagaaaaaa tgtgaaagat tatgaagatg ttgtttatat
     5221 taaactagtc aaaaaagaac ttgaagaatg gaagaaaaat atattaaaga aaccttctat
     5281 atttgacaaa gcatcaaagg gtatacagaa taaatttaat ggagtattgc cagataaata
     5341 tcatgaaata attacttctt ctataaaagt aatgacaaaa accgtactgt ttggatataa
     5401 atatgctaca aagccaccac ttagaaatat gtcattagaa gaaagagaaa aattagtttt
     5461 agaaaaagct aaaacttaca agactacagc aatgattgaa ggggcaggaa caggggcagg
     5521 aggaatcctc ttaggtttat ctgattttcc tttgctttta ggtattaaag tgaaattttt
     5581 atatgatgta gcttcaatat atggatatga tgttaaagat tatagagaaa ggatttatat
     5641 attaaatata ttacagttgg ctttttctag tcaacagcat ataaacaaaa cttttaaatt
     5701 gatgaataac tgggatgaat atgtaaatag tctaccagaa gacatcaatg attttgattg
     5761 gagaacattt caacaagagt atagggatta tttagattta gctaagtttt tacaactaat
     5821 gcctggaata ggagcatttg taggatttta tgtgaatgga aaattaattg ataaattatg
     5881 tgaaactgct gtgtctgctt atagattgag gattcgagag cttaagttat aagaaaaatt
     5941 ataacataaa aaattagtta tacaatatat ctatatgtat ctaattttgc attgataaaa
     6001 acaattatgg ataaaaggta aagcatgata gaatctaatg tagaggtttg tattttaatg
     6061 gaaaaattta gtttttattg cttaagatac aaattgaatt acgaagagat tgaattatga
     6121 agagaagtaa ttgggaggaa tattttcatg acaaatttgc caaaacaatt tgatagcttc
     6181 aatgaagcta gacaaaaagg atttataaat gcaaaggaac ttaaggaaag tgggaaaaag
     6241 atggttggag ttttctgtac atttactcca gttgaaattc caatggcagc tggagcaact
     6301 gtagtaggtg tatgcggcgt tagtgaagag ccaatacctg atgcagaaag ggtgcttcca
     6361 agaaatcttt gtcctttaat taaatcaagc tatggacatg caataacaga tacctgtcct
     6421 tatttctatt tttctgattt gttgatagga gaaaccactt gtgatggtaa gaaaaaaatg
     6481 tatgaagaac ttgcaaaagt taagccaaca tatgtaatgc atttgccaaa tacttcaaaa
     6541 ggagaatttg cctataaact ttggaaagat gaaatgataa gattaaaaga agaggttgag
     6601 aaatctttag gtgtaactat aactgaagaa gatataagaa ctgctataaa agataaaaat
     6661 gaagaaagag aacttttaaa agagttttat gcacttggta aattacaacc atctgcactt
     6721 acaggccttg agttacataa tgttttatat caagcaggtt ttaaatttga ccgtgcagaa
     6781 cttaaacatt ctttaagaaa agttatagat gatatgaaag aaagatatga aaaaggtgaa
     6841 tgtcctgttc aaaaagataa gccaagaata ttgataacag gttctcctat tggtggaata
     6901 agcgaaaaaa tagttaaaac tttagaagat gcaggggcat cagtcgttgc atatgaactt
     6961 tgtggagcta ttagatcaaa cgatttattg gttgatgaag aaatagaaga tgtatatgat
     7021 gctttaacac aaaaatatat aaatataggt tgttcttgta tgatgaataa tgacaatcgt
     7081 attgaattat tagatagaat aatagatgaa tataatgtag atgctgtaat agatgtagtt
     7141 ttacaagcat gtcatacatt taatatagaa agttatagaa taagagaatt tgttacaaaa
     7201 gaaaaaaata agccatttat gtctcttgaa actgattatt caaaatcaga tactgaacaa
     7261 ttaagaacta gatttgaagc gtttgtagag atgttgtagg aataaataaa agtaggagga
     7321 actatgttta gtataggagt tgactcaggg tcagtggcaa caaagggtgt gctatttgat
     7381 ggtgaaaaaa ttattaagaa aataattata ccaactggat ggagccctaa aagtacatct
     7441 aaacaggtct atgagttgct aagtagtgaa attgacaaaa aagatataaa aaaggtggta
     7501 ggtacaggtt atggaagggg agtaatggat tttgctgata agaaagtaac agaaataact
     7561 tgtcatacta gaggtattta ctttttaaac aagaacataa ggactatttt agatgtaggt
     7621 ggtcaagata gtaaagttat aaatttagat agagatggaa atgtatttaa ctttataatg
     7681 aatgataagt gtgctgcagg aactggtcga tttttagaga ttacttctaa ccttttagga
     7741 agcgatattg aaagtataga tacattagct aaaggatatg aaccagttaa tatatctagt
     7801 atgtgtacag tttttgctga gtctgaaatt gtaagtcttc tagctcaaaa catttctact
     7861 ggagaggtag cagcagggat acttaaatct attgcaaata aatccacctc aatgttagca
     7921 agaggtgagg taatagatga agttgcattt actggagggc ttgcaaaaag taaagaacta
     7981 gtaaaaatga tagaagaaat actgggtaaa aaaattttta ttgcagaaga tactcaaata
     8041 ataggagctt taggagctgc tgtaatagga tttagataaa aacatataaa aagaaaatga
     8101 gaagctgaat ttattataaa taaattcagt tttttatttt attttattta tttataaagg
     8161 aaaactttgg tataattgat ttaaatgatt gtatatttgt aaatacttga tatttagtca
     8221 tatataacat tataatttta aaaattagct caaaataagg gggagaaagc gtgaaaaaat
     8281 ttacttcaaa aaaagtaaca aggtttgttg cgctgttttt aatatctatt ttagtaatgt
     8341 caacaattcc tgtatcagct gacaatagca atacattgaa tcagataaaa aaagcagaat
     8401 acagtgagac gtataaacaa tatctaaatg atgctaaaaa tggatgtaca gaaaaatata
     8461 atgggattat accaaatcca tatcagttgg aaggtactca aatccaaagt aaaggaagaa
     8521 cagctccaac atcttatgac cctagaaaat taggtcttat gacatcaata aaaaatcaag
     8581 aggatttagg aatctgttgg gattttgctg caatggcaac cttagaaagt ttcttaaagt
     8641 taaataatta tggagactat gatttatcag aagaacactt aagatggtgg gcttcagatg
     8701 gtgaatatgg atggtctgta aatgatatga atggtgcttt aaattatgaa gctatgggat
     8761 atttaacatc ttggtcaggt cctaaattag aaaaagatat cccatacaat ggtagagtat
     8821 ctaaagcaca aggagcaaaa aaaccaacta atatgaatac agcacctact gtttttaatg
     8881 tgacagatgc tgtgtgtgta agcaatgaca taaattctac aaaaaatgct atattacagt
     8941 atggagctgt aacatctggt tattatgaag atattaaata tcaaagtgat gaccaaaatt
     9001 catattattg tccaacaaaa agttttaata caaatcatgc tatttcaatt gtaggttggg
     9061 atgataacta ttctaaagat aattttaatg caaatattag accatcaaaa aatggtgctt
     9121 ggttaataaa aaatagctgg ggagattata attctgaggg tggatatttc tggatatctt
     9181 atgaagacaa gacacttatg tctgatatag ataatttttc aatcaaaggt ggtaaaaaac
     9241 caaatgatga tgagaagatg tatcaacatg actatgcaag tattgtacct ttaatttcaa
     9301 aaaagataac agctgcaaat gtatttgatt tcaatagagg cgatgaaact ctaaaatctg
     9361 taatgttctt aactgaaagt ataggagcta agtatgaagt atattatgct ccagtagtaa
     9421 atggtatacc tcaagaaaac aatatgaaaa aattaaaaga aggtactgcc caatattcag
     9481 gatacataac agttccaatt gattcttttg atataccaga aggaaagggt gcaattgtag
     9541 tcagtataga ggggaaaaat ggagagtcaa ctataggttc tgagtcaaat gtaccaggat
     9601 atgatacatt taaagcaaaa gctaatttag gtgaaagtta tatcatagat aatactggaa
     9661 aattctttga tataaataga gatagtaact tctatccgtg taattttact ataaaagctg
     9721 ttacagaaaa gtcttctggt gagagtatcc caaatgagtc tttaattggt agtgatagat
     9781 atgaaactgc tatcaaagtt agtcaaaatg gtttcaattc ttcagaaaat gtagttttag
     9841 taaatggcag ttcaatagta gatgcattgg cagcaactcc ttttacatct gctataaatt
     9901 caccaatatt attgacacaa aaagaagctt taaattcaaa aactaaagct gagatacaga
     9961 gattaggtgc taagaaagta tacctaatag gtggagaaaa ttctataagt aaagaaattg
    10021 aacaacaatt aaaaagctta aatatatcga tagagagaat atcaggaagt gatagataca
    10081 agacaagttt attattagct caaaagttaa atggtataaa aaatgtatct caaatagcag
    10141 ttgtaaatgg agtgaaagga ttagctgatg caattagtgt aggcgctgca gcagcagaaa
    10201 ataacatacc aatcatactt gcaaatgaaa agagcgaatt gcaaggagct gatgaatttc
    10261 taaattcatt aaatatagag aaatcttata ttattggagg aacagcatcc ctatcaaaca
    10321 atctagaaag taagttaaaa aatccaacta gattatctgg tagtagtaga gacgaaacta
    10381 attcaaagat aatagataat ttttataaaa aagatacttt aaaaaatgca tttgtagtta
    10441 aaaatggaat caagaatcaa aatgatttaa tagatggatt ggcagttggt cctttaggag
    10501 caaaaactgg gtcaccagta attttagttg gtgataaatt agctgatagc caaaaagaag
    10561 ttcttaaaaa taagacttta gaaaaagtaa ctcaagttgg tggaggagca aataaaaatg
    10621 catttaaaga gttagtaaaa ttaaaaagtg ctaaatagag tacttaagta ttaacatata
    10681 ttagtgtgta ttagtaactt atcttaataa aagctgaaaa taattacttc aaattattga
    10741 taaataagat aatcaatgat tgtaaagttt tgttttcagc tttaaattta tgtagtataa
    10801 gtttaaaaac ataaaaaagg gttactttat atagaaatat agaaaatctt acattttata
    10861 tataaataac ccatatttaa ttaacttttt atagtgttct aatagatatt attgaactta
    10921 atggtaaata taatgtgtaa gtttcattta aagttggatt agattcatct atatatcttg
    10981 agcaaagtgt aattgataag ctatctgctt ctataatttc accatataga ggacaactgc
    11041 taaagtaaga atgtacttca acatatttat ttaagttttc ttgaacataa tcgtaaaaac
    11101 ggttttttgt agtagtagcc ataaaatcaa ctcctttatt ttttattaaa gttgaaactt
    11161 ttaaaaagat aaatatgatt acttctatta gaaactaagt taaaaaataa gcaaatagat
    11221 atgaaaatta gaataatata ttatttatat cttaattata acactagata ataaaaaatg
    11281 ctatataaaa ttcagaattt tcgaaagttt ttaagcttat ttttaaatat actaataata
    11341 ctattgacat tagatagaaa aagtattata ttgactatat aaattatttt agtcaaactg
    11401 gagggaaaag atgcctaaat catattctga taaagaaaga gagtatataa taaaaagact
    11461 taaagaagaa gcaagattgt gtatggagca atatggtatt agaaaaacta ctgttgatga
    11521 gcttgtaaaa cgggtaaaaa taccaaaagg aactttttat ctattttttc aatcaaaaga
    11581 attactcttt tttgaggtat taagagatat acatgactct atacaaaaag aaatcttata
    11641 tgagataaat agagtagatg aaagtattac atgtgaacaa ttgacagata tatttatgaa
    11701 attctatagg atggttgata gtacttcaat tttaaatctc atgataaatg gagaatttga
    11761 gatattggtg agaaagttac cagattctat aattgaagaa tactttagac atgatgactt
    11821 tgaaattgga gagattatat cttgcatacc aaatgcaaaa aataaagaca tagaaagctt
    11881 tagtggagca tttagagcag tttttcttac catgctatat aagcgcgagg ttggaagtaa
    11941 ttgttttgaa gatgctttaa gattaatgat aaacggatta gttatacaac ttatggaata
    12001 gaatttaaat tttgtttgct aataactttt attaaaaatt attaacaaac aaaaaaatta
    12061 tattttattg accatatagt caaaatagtc aaaaatttga aagggtgata ttgaatgatt
    12121 aaagttgatg atttatcatt tagttataca gatagagatt ttttacaaaa tattaacttt
    12181 gaagtaggaa aaggggaaat actaggattt ttaggaccat caggtgcagg aaaatctaca
    12241 ttacaaaaga ttctaattgg aatgataact aattatggag gaagtgtcat tgtaaatgga
    12301 gtagagagta aaagacactc aaataaattt tatgaaaata taggagttga ttttgaattt
    12361 cctagtttat atgaaaagtt gactgcaata gaaaatttaa aatattttgg gtctctatat
    12421 tctaaaaaac tactatctat tgatgagtta ttaaaatcag taggactaga aaatgaatca
    12481 aataaaaggg tatctgagta ttcaaaaggt atgaaatctc gattaaattt tataaaagct
    12541 ttattacata atccagatat tttattttta gatgaaccaa caagtggtct tgacccatca
    12601 aatagcaagg ttatgaaaga tataatttta tcagaaaaat cgaaaggtaa gactattatt
    12661 ttaacaactc ataacatgtt agatgcaaca gagctttgtg acagagtagc gttcattgta
    12721 aatggtaaaa tatctgcttt agatacacca cataatctta ttatgtcaaa aggagctatt
    12781 aaagttagat atacttactt tgataatgga gaaaaaactt ctgaatgttt tctgaacaat
    12841 actgctaatg ataaaaattt gaatatgctt attgagaaaa ataaactttt atcaatacat
    12901 agtagtgaac caacattaaa tgatattttt attgagataa cagggaggaa tttacaatga
    12961 ggctaaaaaa tcttatttta ggagatataa agtttcaatt taagtatggt ttttattttt
    13021 tatatctttt tttaagtatt atatatattt gtattatcaa tgtatttcca acttttatga
    13081 gagaaaaaat agctataatt atgatttatt ctgacccagc agcaatggga ttgtttttta
    13141 tgggagctat tgtacttctt gagaaaagtc aaagggtttt aaattcctta gctgtatctc
    13201 cagtaaaagt atctgagtat attttatcaa aggtaatttc attaggagta atatctagta
    13261 ttgtagctat gtttattgct attacattaa atttagataa tattataata tcaactatag
    13321 gtaccttttt ttcttctatt atattttctt tgcttggatt gataattgca agtaaagcat
    13381 ctagtttgaa tcagtttatt gttttaagta ttcctattga gattatatgc tttattccac
    13441 caatactgaa tgtacttctt gacacaaaat catatgcaaa tttgtaccct tttaatatat
    13501 gtatttctct tattagtgga gataaaaatt ttataatgat taatatttta attttaatat
    13561 caattataat aataatttat tttataactt attattttat ttgtagcagt tggaaaaagg
    13621 taggtggagt aaaactatga taaagattat aaatgcgttt aaacaagagt ttgaacaaat
    13681 taagagagat gctatgcttt ttatagtatg tgtttctcca atattatgtg gagtatttat
    13741 aaaatttgga ataccattaa tacagaatat atcacttaac aagttttatt atcaattaaa
    13801 tttagagccg tatttcttga tgtttgattt acttttagct tttattacac cttttatgtt
    13861 cttttttgct tctacaatgg tcatattggg agaaattgat gacagcattt caagatattt
    13921 aatagtaact ccgcttggta aaactggtta tcttatttca cgatttggaa taccaggtat
    13981 cttagcattt attattacta tgattctact tatctttttt tctctaacta aaatttctct
    14041 cttgttaaac ttatcaattt ctttattagt tttattacaa ggagttatta tatcaatatt
    14101 agtgatttct ttatcatcaa ataaactaga aggaatggta attacgaaat tctcaggtgt
    14161 ttttatgatg ggtattttag caccattttt tattttaaat aaagcacaat atattctgtt
    14221 cttcttacca actttttgga tatcgaaggc atttaaggaa gataattatg tctatatgtt
    14281 tatttcaatt gttatatcat taatatggat tttattatta tttaaaaagt ttagtaaaaa
    14341 aatagttaaa taagaaaatt taatatataa atgtaaggtt gacttgacag taatgttgaa
    14401 aaatgttaca tttaagatgt aaagttaacc ttacatgaat ggagcaaata tgaaaaataa
    14461 aataaagata ttaagagaaa aattaggatt gacacaagaa caactaggga gattagttgg
    14521 aacatctaga caggctataa atgctataga aactggtaaa aatgaacctt caatatggtt
    14581 ggcatatgat atttcaagaa tttttaatga accaattgaa agtgtatttt tatttgaaga
    14641 aagtgaaaga aaatcaagag cacagataag taggggagaa tattatggct ataagaaaga
    14701 taagaacatt tgatgatgaa attttaagaa aaaagagtaa gtatgtagaa aacgtagata
    14761 ataagataag agaaatatta aatgacatgg cagagaccat gtacaatact ccaaatggag
    14821 gaggattagc tgcatgccaa gtaggagtat taaagcgatt agttgttata gatttaggag
    14881 aaggacttat taagcttgtt aatcctgaaa ttattgaaca agaaggggaa caaattgttg
    14941 ttgaaggatg tttaagtttt cctgaagtat ggggtaaatt aaaaagacca aaaaaagtta
    15001 cagtgcaagc tctaaacgaa tacggtgaga aaatagaaat taaaggttca ggttttatgg
    15061 caaaatgtct ttgtcatgag atagaccatt taaatggtat agtttttaca gataaaatta
    15121 ttgaacatgt aaaattgtaa gatagattgg ttattgaatt tataaaatat tttatagtaa
    15181 ggagttaaat tatgagatta tggcataagg atttaattga tgtattacct aaaaatcaat
    15241 tagtaagtca atggagagag ctacttgcta taaaaggttc tatagataaa aaaggtactc
    15301 caaaccatct attagtgaat aaagtattga attatagtat agatgaattt aaattttata
    15361 caaaaattgt acatgatgaa atgttaaaaa gaaattataa acctaatgaa cttaaatata
    15421 caagtatatt aaaatggaaa aatagaaatt ttgcaaatga tatatctaat gaacatagtt
    15481 taaatttaga aaatttatat gatgattggc ataataaaat gtatttaaag caatgcttat
    15541 ataatttaga agaaaaagca acatgtggag gaataccaat aaatgaatgg aatatattac
    15601 tttgtaaata tagtaaagat tatgaattgt ggtctggaaa tatcatgttt taattactat
    15661 agattaacat tatagtatta ttgtatataa agaggtgatt atttgtataa taaaataaaa
    15721 tatattccaa taaattgtga aatagcttgt aataaattaa aaaggagtat gccatataaa
    15781 tgggatttaa atatttatcg tggatgtgag catggatgta aatattgtta tgctatttac
    15841 tctcataagt acatcaactc caataattat tttgaagaga tttatgtaaa aacaaatata
    15901 gtagaaaaac ttgagagaca acttaaaagc agtaaatgga agaaagaagt aatcaatata
    15961 ggaggagtta cagatagtta tcaaccaata gaagctgact ataaaattat gccagaaata
    16021 ttaaacctgc taataaaata caagacacca gctattattt ctacaaagtc agatttaatt
    16081 ttaagagatt acgatttaat tgataagcta tctaggatta catatatcaa tgtagcatct
    16141 acaattacca cagtaaatga agatacacaa aggcttattg agccaaatgg tgtggattca
    16201 atgagacgat ttaaaatgtt aaaagaattt agaaaaacta acgcatcagt tggtctacat
    16261 attatgccaa ttataccata tattacagat gattttgata atataaattc gctatttaga
    16321 catgcaaaag aatgtaatgt acattatgtt ttacctggga ctttatactt aaggggtata
    16381 actagaggtg ttttttttga ctttgttaaa aaagagtttc ctgaattatt tgataaatta
    16441 tcaatactat atagtgcagg ttcacttaat agggagtata aaaatcaatt gtataaaatg
    16501 gttaatgaac ttagatataa gtattctcta tcaagcagtt actctaaaat aatgaaggaa
    16561 aagttaaaaa actcaggaga tgtacagata tctttttttg attagtttta tataaaaaaa
    16621 ttttttttac tatgatgatt aaaatttatt aaaattaaaa tttattgaca aatataaaat
    16681 ttaaatatat aattaattca taaaataaaa atatgaaagg atgatgttgt tcggtgatat
    16741 ttgatatcat aaaatcgttg atataagtat tgctggaaaa gagtgaataa aatcaaattt
    16801 aataagcaaa caaagtaaag ctatagtttt attgttgtgc ttaaaaatat ttggttctat
    16861 tttactctta tttaaactca tctttttgaa gttaaaccac agagtaaact ctgtggtttt
    16921 tttattttaa aaataaattt taaggagtgg tatgttttgt taaacaaatt atgtatgtat
    16981 ttagaaagac cagagcttta taaacaaagt gaaattaatt tttgggatga tgagtatatt
    17041 tcaaagcaat tattaaaagc acacttagac actaattttg aaggagcaag tagaaatttt
    17101 aactttatcg aggattctgt aaattggata gttacagttg caaatccagc taattatcct
    17161 aaattacttg atttaggttg tggtccaggt ttatatgcag agaaatttgc acaaaaaggt
    17221 tataaggtaa ctggaattga tttctcaaaa cgttctatta actatgcaca aaatagaaat
    17281 aaggagacta atttaaacat aaattacttg ttccaaagtt atttaaatat gaactataat
    17341 gaggaatttg atttggctac tttaatttat tgtgattatg gagctttgtc aactgaaaat
    17401 agaaggttat taatggaaaa aatttatgat agtttaaaac ctggaggaaa attaatatta
    17461 gatgtattta caataaataa atacaataat tttgaggaaa ttaaatattg ggaaataaat
    17521 gaagatggag gattttggag taatgaaaaa tatatgtgtc tacaagataa ttgtaaatac
    17581 aatgattata atacattaga acaaactctt gtaataaccg aaaaagatga aaatgtattt
    17641 tatgtttgga atcactattt ttcaaaagaa agtttcttat tagaagttga aaatattgga
    17701 tttaagtctg ttgagttttt tagtaatgtt aagggtgaag catattctga cgatagtatg
    17761 actatgggat tagttttaca aaaataataa tttttttaaa atgaataaat aaaaacttga
    17821 aacctttaac aataaaataa cataataaaa gcttacatat tgaaaaatgg aaacttttat
    17881 gttattttta gtacattaca aatttgctta tataaaaatc aatacttgaa ttattatttt
    17941 tatgtgaaat ttatctatag tatattttta taaacaagta atgttattaa aattaagtat
    18001 gtagttaaaa gtgtatagca aaaaggctgt tatattaatg ttagaggttc agggaaaata
    18061 tataaagcaa agtatttaca gataatgtag attgcacagc tataagttag ataatagaat
    18121 tatgtaatca agaatttaca acaaagtata agataaggat aatgctaaat tgccatacag
    18181 gtgctagatg tgtaaatgga tttaattaat actattgata aagtgcaaca agcacataaa
    18241 ccaattgatg agattattaa aactataaga gatatagttg acataattaa aatattggaa
    18301 cttgtttata attttaaatc aaatcaataa tgattaagat taaaataata ttaaggcatc
    18361 aatgttataa gagtacaata taaggagtac tatatagtac tccttactta tacatattag
    18421 gcaataggaa attttttcat agttaaattt tcaagtattt tttaggaaac tttataattg
    18481 agaattaagg gtatagtatg tgatgaactt actgaattta taattaggtt atataatgga
    18541 ataatcatca atagttttac tttagggaaa atatatgctt aaggaagtat agcttactaa
    18601 tattattaaa atgtatattt aaaatgataa aaaaagaatt agacaagtat ttagtaaatt
    18661 gactatgcaa aattctatca agcaggaacc ctatgacttt agtcatggga ggttcagtag
    18721 gtaacttgtt gaattaagtt tatattattt aaataaacta cttaatttag ataatataaa
    18781 aagtcagaat tttaagaata gagctatata atcttagcta ttaggagaat tactatgata
    18841 ggaatatatt ttagtggaac aggaaattca agatactgtg tagaaaaatt tttacaagaa
    18901 tatgatataa cagcaaattc ttattctatt gaagataatg aacttttaca gcacatcaat
    18961 aatcatgaga atattatttt tagttatccg gtgcaatata gtaatgtgcc aaaaatattg
    19021 aaagatttta taataaatca ttcaactttg tggaatggta aacgtgtttt tattattgca
    19081 actatggctc tatttagtgg agatggctct ggtgtgttat ctagaatatt aaaaaaatac
    19141 ggtgctataa ctgttggtgg attacatgtg aagatgccag atagtatagg agatgaaaag
    19201 gtattaaaac attctttaga gtataataga aagcttgtaa taaaagcaga aaagaaagta
    19261 actaaagctg ctaaaaaact gaaacatggg tcaccaccaa aagaagggtt aggttttttg
    19321 agtcatattg tagggttatt tggacaacga ttgtatttta tcaacaaaac aaatatgtat
    19381 acagataaac taaaaattaa tactgaggaa tgtattgtat gtggtaaatg tgttaattta
    19441 tgtccaatga agaatctagt aattaaaaat tgtatgattg ttgcaaatgg gcaatgtaca
    19501 atgtgttatc gttgtgtaaa taattgtgct aaaaaagcaa ttacattgtt aggtaaaaaa
    19561 gttgttgaac agagtaatat taaaaaatat atgtaagaaa tttaaagttt tattgaatgt
    19621 aaattgatgt atattttatg ttagaaagag aggttaaata atggaatact acatagttga
    19681 ttcatttgca actaaactat ttaaaggaaa tcctgctgga gtttgtgttc ttgacagaag
    19741 aataccttta gagttaatgc agaaaatagc agaagaaaac aatttaccag aaacagcatt
    19801 tgtagttaaa ggtaaaggaa attatgaatt acgatggttt actcctaaag cagaaattga
    19861 tttatgtggt catgcaactt tagcagcagc ttatgtaata tcaaatttta ttgatgttaa
    19921 tgtaaaaagg atagatttct ttactcaaag tggaaagctt gaagttacac gaaatggaaa
    19981 tctatatgaa atgatatttc cagaaataat gcctattgaa atagagttat caccacaaca
    20041 agctaatttg ataggctgtg taccaagtgc tgtttattct tcaagagatt taatcttatt
    20101 gctaaacagt gaacaagaag ttataaatta taaacctaat tacgcacaat tgcgtaaatt
    20161 gacagactgg ttaggtataa taatcacagc acaaggaagt aatacagatt ttgtatcaag
    20221 atatttttgt ccagaattag attcagaaga ccctgttact ggttcatcac attgtaatct
    20281 tattccatac tggtctgaaa agttgggaaa acacaaaatg gtagctgctc aactttctaa
    20341 tcgtggtggt ataattcagt gtgaagtgtt gaaggataat actgtgaaaa tttctggtga
    20401 agcagtcttg tttatgcagg gaacaataaa gattgatatt taaataagta taataactaa
    20461 tttattatga aaaataatta attttcttag tgttaacaat aataaagtag agttaaaaaa
    20521 cttgatttca cttaaatcaa tatgatagat gttgatacta agattgacaa gcatgattaa
    20581 aaaagataat atttaatcta taaagataaa aacacagtct ggttggtagt ccagacgcag
    20641 caatagctgt cagtaacctg cctccttggt tgtccgttct ttagaaagta atttaaggag
    20701 gaactagcaa tggacaaaat tagtggaaaa ttaacagtct tatttgaaga accattttgg
    20761 gtaggtatat ttgaaagaca agatggtaaa aaatatgaag catgtagggt agtttttgga
    20821 gcagaaccta aagaagtaga agtgtatgaa tttatacttg aaagattttt ctcattagat
    20881 tttggtagca ttaaattaga aaaaaatgtg accaaagaca acattggata caaaagaatg
    20941 cagagaaaag taaaaaagga acaagaaaaa gaaactatag ggactaaggc tcaaaatgct
    21001 cttaagcttc aatatgaaga aagaaaacag gatagaaaaa acctagctaa aaatagaaaa
    21061 gaagaagaaa aagaaagact atttaatttg aaacaagaaa agagaaaagc taaacataag
    21121 ggtcattaat tttaattaaa tatcttttta ataattgcta gaaaaaggtg tataaaattt
    21181 ttaattttat acaccttaat ttattgaaaa gaataaggtt ttatatttca attactttaa
    21241 aatataaaaa tatactaata aaaattgtaa aaatagtata aatggtatac caaatgtaaa
    21301 tttaatgtgt ttagttttat gtctaaaaga atacattcca attatagaac ctatactacc
    21361 tccaattact gctatagaca taagagtagc ttcttttatt ctccattcgt ttctaattgc
    21421 tcttttctta tctatgtaca ttgaaaagaa agcaatagaa ttaatcacta taaaatatat
    21481 tagaataaaa tttttcacag tcttgcctcc ataaaaatat attatacatt ataaaacaag
    21541 atatttgttt tggaaagtta aatttgtaat tagtacatat aaaatatttg ataagaacaa
    21601 atataatatt aattggaaaa tttaaaaaaa ttaaacaatt agcaaatagg gggtatattt
    21661 ataagacaca aagtgataaa atctatttag aaaataatgt aaaataacag gaggataaag
    21721 caaaatgaaa tttatagtag aaaaagaagt ttttgataaa ttagaaaatg tatgctttgg
    21781 tgtagtagtt gcaaaaggaa tagataatac taaagaaata gaaagaataa gcaacctttt
    21841 agatataagt atagatagag tagaagatta cttcaaagat aaaaaagtaa aggaatcaga
    21901 agaaattata ccatatagag aggcatttag aagtctaggc atgaacccaa ataaatttat
    21961 gagttcgata gaagcaatga caacaagggt tgctaagaat aagaaacttc ctcatataaa
    22021 tcctattgta gatttaggaa actctatttc tttaaaatat ttacttccaa tgggtgctca
    22081 tgatatggat tttagaaatg atgatgtata tgttagattt tctaaaaaag gggataaatt
    22141 tgtaccattt ggagaaactg atgttgaatt aatggaagaa ggagaattaa tttattcagt
    22201 tggagacatg gtaaaaacta gaagatggat atggagacaa ggagaagaag gaaaaattac
    22261 taatagctca aaaaatatat tttttcctat agatggtttt actgatgcta atttagataa
    22321 ggtaatgagt gcaagagaag aattagcaaa attgttaaag gaaatattta attgtgaaat
    22381 taaagtaggt tttgtagata aagataatcc tgaaatggaa atataataag ttgttgaata
    22441 cttgttaaga tattatattt acttaatgat gttaaaaagg tattaaaaaa gaaaggtcgt
    22501 agtaaatttg aaaaaaacta taataaagaa tgtaaatcca atgattgaaa ataagtttat
    22561 aggacttttt gaaatagagt ataaaaacaa attagatgaa gataaggttt ggatggtagc
    22621 atcaagaaaa agtagtgagc aattaaaaaa tatttatttg aagaatgaag aagatagtgt
    22681 agatgcagtt gtaatagttg ggcttcataa aagtagtaaa aaattaattt taataagaca
    22741 atttagagta cctattaatg gatatatata cgagttacca gcaggtcttg ttgatgaagg
    22801 agagtctata gatatttccg ttgaaagaga acttagagaa gaaacaggac ttactctttt
    22861 agaaattaaa aaaaataaaa gtattgataa agtttattta tctcctggaa tgagtgatga
    22921 atctgttgcc tttgtttatt gtatatgtga tggtgatata acagatgaat ttttagaacc
    22981 agatgaagag attgaagcgc ttttaatttc gcaagaagat gcaaaagaga ttttacaaag
    23041 taatcataag atagacacaa aagcattttt gattttacaa atgtttgtga gtttaggagc
    23101 taaattattt gaatagatat atagaaaaga ggttatatag aagagtaaaa taataaagtt
    23161 tattttactt gtgtaaatta taatctcttt ttttatacaa taaagtataa ttttgtgtat
    23221 ttttaaataa gtgatttgac tcttaaattt tcataatatg ttataaacca atttaagata
    23281 ctacaataat taaaattcca aaattattta acatatttgc aattaaaatg agaaaatgta
    23341 aaggctgaat gattgaaaaa tttgatgtaa aatagtagct tagtttaata ctatagtttg
    23401 tagggttaaa aattagaaat tgtaagggag ataaattcta tgaaaaaatt agttattatt
    23461 ttaattagta tcattagtat ggttagcttg acagcttgta aaaaaagcaa tgaaagtaaa
    23521 tataataatt taaacaacaa agaatccgga caaacaacca ttgaaatgga cttagataaa
    23581 aattatgata cttcagaccc atttgttaat gcacgtttgt tttgtgtgtc taatgatata
    23641 gatgttttag acacagagat ttcttttaaa atggatggag atagtggtat tgtggaaata
    23701 aaggacaata aaacagatga aactttatgg agtaatacat ggcatggaag cgttgataat
    23761 gatacattca caatttctct ttctaatata caaaaagaga aagagtatgc tatatgcttt
    23821 actggaatta aaataaatca tgcagttgta aaggttactt ttgaaagcaa tttagttaag
    23881 gagaaagaaa aaccctcaaa ataaattcca gcttttctat aacttgatat tcagtacata
    23941 aaagcagttg atatttatga ttgactgctt tttcttatca aaaaggatag gttgtgcatt
    24001 tttacttgaa atagtttatg cacatcaaaa gttggtgagc atgatattgg aaatggtaag
    24061 catgaaatct tgataataca aggaaataaa tttaagtttt caattaagta tatttccata
    24121 atttagttaa caataagcta attattattt atattttgat atataaataa taattgtagc
    24181 aaattttaaa aaatcatata ttttttgaac taaataaaaa tttattttta ttattttaaa
    24241 ttagaggaga ttcttgaaga tatattattt ttaaaaataa gtatagttta aaaattcaaa
    24301 aaaaagatgg caaaatatgt cccaagggac attgagtaaa ataaatgatt tcagcttatt
    24361 tacactaaaa agtacatact aaatgagggg taaaataaag aaaaatgata tactaattgt
    24421 cttttgataa taaaaaaaca tggaatttga ttatttaatc aagaaaaagt attgaacaaa
    24481 tgatttattt agtgtacaat aatcatgtaa aaacatagta atggagggag acatttcata
    24541 atgaaaatta aggtaggaat gaacggattt ggaagaatag gaagagctgt attaagaata
    24601 gctcaagaag aattaggaga taatatagaa attgtagcca taaatgcaag agcaacaaca
    24661 gaatctttgg cacatttatt tacatatgat tcatgctatg gaacttttag aggtgaagta
    24721 gaggcaaagg atgaagacac tctagtagta aactctaaag agataaaaat attaagatat
    24781 aatgacccag aagaattacc ttggaaagaa ttgggggttg atatagtaat agaatcaaca
    24841 ggattattta cacaaagaga aaaggcagaa aaacatatta aagcaggagc aaaaaaagta
    24901 attataacag ctccaggaaa aaatgaagat ataactatag ttatcggagt taatgagaaa
    24961 gaatatgata atgaaaagca caatataatt tcaaatgcat cttgtactac aaattgtcta
    25021 gctccttttg ctaaagtatt ggacgaaaaa tttggtatag taaaagggtt aatgactaca
    25081 gtacattcat atactaacga tcaaagaata ttagataaaa gccataaaga tttgagaaga
    25141 gcaagagcag cggctgaatc tataatacct accactacag gagcagcaaa agctgtgtca
    25201 agagtactac cacaattaga aggaaaatta aatggttttt cacttagagt accaacacca
    25261 acagtttcac ttgttgattt agtgtgcgaa cttaaagaaa acgttacatc tgagcaagtt
    25321 aactctgtgt taaaagatgc tgctgaagga gaattaaaag gtgttttagg atattgtgac
    25381 aagccacttg tttctataga ttataaagga gattcaagat catctattat agacgctcta
    25441 tccacaatgg ttatagaaga caatatggtt aaggtagttt cttggtatga taatgaatgg
    25501 ggatattctt caagaactgt tgacttagta aaatatgtag ctgaaaaatt aaaataatat
    25561 atatttctta aagcaaattt taaaaaaatt ttttaaaaaa attatataaa actaaaaaaa
    25621 aagattatga aaaatattga aggaaaaatt ttttcataat cttttttctt tcatgcaaaa
    25681 agatacaagt tacaacttat atttgcatat taggtcgtta gagaagtata tatatattta
    25741 gtaaggaatc atacttttta taaaatgaaa gataagatgt cgaaaacttg cataaatctg
    25801 aaaaattttt attattaaaa tactgatttc cattttaaaa atgataaaaa aatgacaaat
    25861 aaagttgcaa atagtaatac ttataagtat aattaaaaaa gagaaaaggt tttgttaaac
    25921 tttataacaa aaaaagaagg gatgattcaa aatggagaag ttaacagaca ggagacaggt
    25981 taaggcagta agcatggaaa ccttcatttt cctaatatta ctagtagttg gttttgggta
    26041 tgttggtagt attatgggag caggaatgat gttcaaagta ataatgagta cagcacatgc
    26101 tttactattg gagacagttt tcttaattat ggctatggca gttcttgcag gcgcattaag
    26161 cgcactactt tcagagtttg gagtaatagc gttaattaat aaaattttcg ctgtatttat
    26221 gaagccttta tatggactac ctggtgctag tatagcagga gctataacaa cttatttatc
    26281 agataatcca gctattatac cttttgctaa agataaaaca ttcacacagt attttaaaca
    26341 ataccaagtt ccagcattat gtaacttagg aacagccttt ggtatgggac ttatcttaac
    26401 aacatttatg atttctcaag gtacagaata tgtacttcca gcattaattg gtaatttagg
    26461 tgcaattata ggtagtgtca taagtgttag aataatgttg acatttacta agaagtttta
    26521 taaatacaat ccggaagaag ataaagcaac aggaacatta gagaaaaagg atgaatttag
    26581 agaaatcaga gaaggaaatg tatttcaaag agctttagat gcaattcttg aaggtggtaa
    26641 aatgggggta gatatgggaa tggctataat tcctggagtc ctagtagtat gtacattggt
    26701 gatgttatta acttttggac catctacaga tcctgttaca ggtcaagaag tttatacagg
    26761 agctgcatat gaaggaataa aattacttcc agttattgga gataaacttg gatttatatt
    26821 agaaccatta tttggattta catcaccaga ggcaatagca ttccctataa cttcattggg
    26881 agctgttggc gcagcaatgt ctctagttcc agagtttata aagtcaggag ctataactcc
    26941 taatgatata gcagtattta cagctatggg tatgtgttgg agtggatatt taagtacaca
    27001 tattggtatg atggatgcat taaatgctag acagttggca ggtaaagcta ttctttctca
    27061 tacaataggt ggtttatgtg caggagcagc tgctcacttt atatttacac tcgtaggata
    27121 aatgtaatca gctaagataa aaaataatat acaaaaaatg gttgttgata tgtaataaga
    27181 atttacatat caacaaccat ttttttctga ctaaagataa tttataatac ataaacatta
    27241 agtggatatt agagttaagt tatatttatg taaaaagtaa caaaaatctg tagaaattta
    27301 tgacaaaatt tagtacacta actatatgtt cttatttaaa atttaaggtt agaattagga
    27361 attacctagt agagtaagct ttgattaata aaagaatcat aaaataaaaa tattagtcat
    27421 aaagattata aataacttac ttaaaataaa gccatagaac ttacaaaaat taaaaaagca
    27481 tataaatatg gaggggtaca atggatgaat tatttcatat aggaattgat gtaggttcta
    27541 caacggtgaa ggttgtggta cttaataata gtaataatat agttcataaa gaatatagaa
    27601 gacattattc tgatgtaaaa aaatcagtta aagaagtact aaatggtata tatgaaaaat
    27661 tgggagatat aaatactacc attataataa ctggttcagg tggaattgga atatcaaaaa
    27721 aattaggtgt aaaatttgtt caggaagtta tatctagtac aaaatcaata gagtattttc
    27781 atcctgaaac tgatgttgtc atagaacttg ggggtgaaga tgcaaagata acatatctaa
    27841 gtggaggaat agaccaaaga atgaatggaa tatgtgcagg tggcactgga gcattcatag
    27901 accagatggc atcactttta aagacagatg ccagtggatt aaatgaactt gctaaagggt
    27961 ataatgttat atatcccata gcttcaagat gtggtgtttt tgctaaaaca gatattcaac
    28021 ctcttataaa tgatggagcc aatcagactg atatagcaat gagtattttc aatgcagttg
    28081 tcgtacaaac tgtgagtgtc ctttcttgtg gaagaaaaat agaaggaaat gttgcgtttt
    28141 tgggtggtcc attgtacttt ttatctgaac ttagagaagc atttaaaaga gtattagatt
    28201 taaaagatga aaacataatc ttcccagaaa atgcacaact ttatattgct attggagcag
    28261 gattattatc agtagatgaa gataatatac aactaaaatc tctaatagaa aaattggata
    28321 gtataaagaa tattgaagat ggagaagtaa atttattaga acctttattt aaagataaaa
    28381 atgagtatga agaattttca agaagacatg aaaaagaaaa aataaattat gtggacataa
    28441 attcaataaa aagcaattgt tatttaggaa ttgatgcagg ctcaactaca actaaagcag
    28501 cacttattga tgaggatgga agacttgtat attcttatta taatagcaat gaaggaaatc
    28561 cattaaaaac aaccattaaa gttataaatg aagtatatga tatattacca aaaaatatta
    28621 aaatattgag ttcaactgta actggatatg gagaaggtct tattaaaaaa gctcttaaaa
    28681 tcgataatgg agagatagag actatagcac attacaaagc tgctaaattt ttcaacaaag
    28741 atgtagattt tatacttgat attggtggtc aagatatgaa gtgcctaaaa ataaaagatg
    28801 gagtaattga taatataata ttaaatgagg cttgttcatc aggttgtggc tcatttttag
    28861 agacatttgc aagttcttta tctatgacaa tagaagaatt tgcgcatgaa ggtatttatt
    28921 ctcaaaatcc tgtagattta ggttcaagat gtactgtatt tatgaattct agagtaaaac
    28981 aagctcaaaa agaaggtgca agtgttggag atatatcagc agggctatct tattcagtaa
    29041 taaaaaatgc tttatttaaa gtaataaaga ttagagattt aaatgagatt ggtaataata
    29101 ttgttgtaca aggtggaacc ttttataatg atttggtgct tagaagcttt gaaaaactaa
    29161 ttggaaaaaa tgtaattaga cctaatattt caggaattat gggagctttt ggttcagcac
    29221 ttatagctaa agaaaaatat gaaagtggat acaaaacatc cttgttatct aaggaagagt
    29281 tgaataatat caagctagaa gcaagtgtaa ctagatgtaa aggttgttca aatcattgtc
    29341 ttcttacaat aaataaattt tctgataatg aaatttttat ttcaggtaat agatgtgaga
    29401 aaggtgaagc tatttatggt gataaaaaaa taggattaga aaagaaaaaa ccaataaatt
    29461 tatttaagta caaatacaat agaattttta gatacaaacc attggaaaaa gaagaagcaa
    29521 aaaatgggga aatagggata ccaagagtat taaatatgta tgaagattat ccattttggt
    29581 ttactttttt caatgctatg ggctttagag ttgtactttc agataggtca tcaaaacagt
    29641 tatatgaaac tggtataact agtattgcat ctgaaacagt ttgttatcca ggaaaattag
    29701 ttcatggaca tatagaaaat ttaattgcaa aaggtataaa aaatatattt tatccaagtg
    29761 taacaaatga aaataaagag gattcaaatg cagataatta ttataattgt cctgttgtaa
    29821 tatcatattc tgaagtaata aaaaacaatg tagagaatat aagaaataag aacataaatt
    29881 atataaatcc atttataagt ctaaatgaca aggaaaaatt gaagaaaaga ctctatgatg
    29941 aattatcaag acatttttct gatataaaaa taactaaaga agaagttaat catgcagttg
    30001 ataaagctac tgatgaacag aatttattta aaatggagat acaagcagca ggtgaaagag
    30061 cacttaaaga aataaaagaa agaaatatga aggggattgt gttatgtgga agaccatatc
    30121 atatagaccc agaaataaat catggaatgc cagagcttat aaattcatta gatatggcag
    30181 tgcttactga agactctata tgtcatttag ccaatgtaca aagaccactt agagttgtag
    30241 accaatgggt atatcattca agattgtata aagcagctag ttttgtaaga gataaaaact
    30301 atttggaact tgttcaactt aattcatttg gctgtggatt agatgcagta actacagacc
    30361 aggttcaaga aatacttaat gaaaaatcga agatatatac tattataaag attgacgaag
    30421 gcaacaattt aggagcagct aagataagga tgaggtcctt aaaagcagct atgtttgaga
    30481 gagaaagcaa aaatatagat gtaaaaaata taaaggtaaa caggatacag tatgctaaaa
    30541 ataatcagat aacgaacaaa catactatat tagcaccaca aatgtctcca atacatttcc
    30601 aattccttga aaaagctgtt aacttaagtg gttataatat agaaattcta aaagatactg
    30661 atagtagtgt aattgaagaa gggttaagat atgtaaataa tgatgcatgt tatccagcta
    30721 taatagttgt aggtcagcta ataacagcat taaaatctgg caaatatgat ttaaataaca
    30781 cttctgttac tataactcag actggtggag gttgtagagc tacaaattat attggattct
    30841 taagaaaggc tatatacgat gctggattta aagatattcc tgtcattgca cttagtgtaa
    30901 atggaataga agatagtggt ataatggata atatatcact aaaattaatc aatagactct
    30961 ttatgtctgt tgtttatggt gatttgctga tgaaagtact atatagagta agaccatatg
    31021 aaaaagttac tggaagtgca aatgctctat atgaaaaatg ggttgatata tgcaaatctt
    31081 ctcttgttaa agcaaaaatt tctgtattta ctagaaatat aaaagatata gtaaaagaat
    31141 ttgataattt agagatacta gatatcaaaa aaccaaaagt aggtttggtt ggagaaatat
    31201 tggtgaaatt ccatccaatt gcaaataata atttagtaga tatattagag cgagaaggtg
    31261 ctgaagcagt cgtgccagac cttacaaatt tcttcttaag ttgtgccttt aatactatat
    31321 acaaacacac tcatttagaa ggaagtagaa agagtagaat gataggagaa gcatttatat
    31381 acataactgg aatatatcaa agagtatata aaaaagcact tgataagagt gagagatttt
    31441 atgctccagc caatataaaa aatgttgcta aaagtacaga accagtagtg tcattaggta
    31501 atcaaactgg agaaggttgg cttttaacag gtgagatggt tgaattatta aatgaaggtg
    31561 tagaaaacat tatttgtatg caaccttttg gatgtttacc taatcacata attggtaagg
    31621 gttctataaa agaattaaag aggttgtata aaaatgcaaa tataatacct attgattatg
    31681 acccttctgc aagtgaggta aatcaactta acaggataaa acttatgctt tctaaggctt
    31741 ttaaaaatat ataagattta tattttgata tgttaaaagt taatgaattt aaaaatatac
    31801 tctatattaa gtatctataa aaaagagtta tcctattttt tagggtaact ctttattagt
    31861 taaaaaatta tatgtatact gatattaaag gggggaagta atatgagttt agtatatgat
    31921 gtttataaat caaaattagg aaatttgtac atattaagtg aagatggttt tatagttagt
    31981 atatacatag gagatgaaaa atttaataat ttaaaaaatg aatgtattaa aaaaggaatt
    32041 gatataaaga gaagtaagga agatttaaag gaagcaataa gccagataga tgattacttt
    32101 aatggaaaaa gaaaagtctt taatttaaat attcgtattg aagggactga atttcaaaaa
    32161 tcagtttata gggaaatgtt aaaaatacct tatggagaaa cattgtgtta ttcagatata
    32221 gcaagaaata taaacaatcc aaaatcagta agagcaatag gacaagcaag taaaagtaat
    32281 aaaattccaa taataatacc ttgtcataga gtagttggca aaaaagatat tggtggatat
    32341 atggggaatc attctgattt gaaagagata ctattaaatt tagaaagaca gagttaactt
    32401 agaaagaaat gtttttattg ttaatatttg atataataat agtaaaaaga agtatttttt
    32461 acaaaaacaa tgaagaattt attttaattt ataaaaatga gggggaattt taatgtgtga
    32521 taaagttata gcatcaaaat atcatgagaa gggatttaat tgtgcagaat cagttataaa
    32581 agcatataat gaggaattta atacagatat accagtttgt ttaggaagtg gactgggttc
    32641 agggtgtggg gttgctagtt tatgtggtgc agtaaatgca tcaaatataa taattggata
    32701 tgtaaaaggc agaaaccacg aagatgaaag tactaaagct aaagtatatg ctaaagattt
    32761 aactacaaca gtaagaaaag agtatggttc agagttatgt atagacctaa aaaaagattt
    32821 agttgcttgt agagaaataa tggatttcgc atatgatagt ctaaaggaaa ctttaaaaaa
    32881 agaattataa gaaaataagc tatcaagtat aaattatttg atagcttatt tttatagtcg
    32941 atactaaata aatcaaatat attttagaat tattatttgt aaataaacag caaataattt
    33001 taggattttt atttaatata gtttttattt atatttattt ttgaatatgt tatactttgt
    33061 ttaaataaat atttgtaatg agggagatga gaagattgaa gaatctcaat ttacttatta
    33121 ataaattata ttcaaaaaat cataatgaag catataaaac ttttttattc ttagaaaatg
    33181 aaagtttaaa atcaaatatt acatattgtt tttttgactc attcctagaa atgattgata
    33241 acgaaaactc atatattcga acaaggggat tgttattaat ctctgcaaat gctcagtggg
    33301 atattgataa taaaattgaa ataaatatag atagtatact aagtcatatc gtagataaaa
    33361 aaccttctgt atcaagaatg tttattaaat ccatacctaa tattacaaag tataaagaaa
    33421 atcttattaa tcgcataaaa atggaattaa gtaatgcaga tataagtatt tataataata
    33481 atatgaaacc attagtagaa aaggatat
//
LOCUS       DAMJWU010000035        33028 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMJWU010000035 DAMJWU010000000
VERSION     DAMJWU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.035"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            215..1036
                     /locus_tag="PC460_001824"
     CDS             215..1036
                     /locus_tag="PC460_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2600711.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1115..1326
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1413..2729
                     /gene="argH"
                     /locus_tag="PC460_001825"
     CDS             1413..2729
                     /gene="argH"
                     /locus_tag="PC460_001825"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF2600712.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            2978..3316
                     /locus_tag="PC460_001826"
     CDS             2978..3316
                     /locus_tag="PC460_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF2600713.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(3443..4732)
                     /locus_tag="PC460_001827"
     CDS             complement(3443..4732)
                     /locus_tag="PC460_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2600714.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            4812..5516
                     /locus_tag="PC460_001828"
     CDS             4812..5516
                     /locus_tag="PC460_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDF2600715.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQRNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            5608..6654
                     /gene="selD"
                     /locus_tag="PC460_001829"
     CDS             5608..6654
                     /gene="selD"
                     /locus_tag="PC460_001829"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5656..5658,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF2600716.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            6672..8084
                     /locus_tag="PC460_001830"
     CDS             6672..8084
                     /locus_tag="PC460_001830"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF2600717.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            8198..10081
                     /gene="selB"
                     /locus_tag="PC460_001831"
     CDS             8198..10081
                     /gene="selB"
                     /locus_tag="PC460_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF2600718.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            10186..12924
                     /locus_tag="PC460_001832"
     CDS             10186..12924
                     /locus_tag="PC460_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF2600719.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(13043..13987)
                     /gene="manA"
                     /locus_tag="PC460_001833"
     CDS             complement(13043..13987)
                     /gene="manA"
                     /locus_tag="PC460_001833"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF2600720.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            14160..15521
                     /locus_tag="PC460_001834"
     CDS             14160..15521
                     /locus_tag="PC460_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2600721.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            15609..17498
                     /locus_tag="PC460_001835"
     CDS             15609..17498
                     /locus_tag="PC460_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2600722.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            17513..17968
                     /locus_tag="PC460_001836"
     CDS             17513..17968
                     /locus_tag="PC460_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2600723.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            18027..18344
                     /locus_tag="PC460_001837"
     CDS             18027..18344
                     /locus_tag="PC460_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2600724.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            18369..19631
                     /locus_tag="PC460_001838"
     CDS             18369..19631
                     /locus_tag="PC460_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2600725.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            19736..20800
                     /locus_tag="PC460_001839"
     CDS             19736..20800
                     /locus_tag="PC460_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2600726.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            20854..22032
                     /locus_tag="PC460_001840"
     CDS             20854..22032
                     /locus_tag="PC460_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2600727.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(22223..23281)
                     /locus_tag="PC460_001841"
     CDS             complement(22223..23281)
                     /locus_tag="PC460_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2600728.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            23348..23719
                     /locus_tag="PC460_001842"
     CDS             23348..23719
                     /locus_tag="PC460_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF2600729.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            23756..24430
                     /locus_tag="PC460_001843"
     CDS             23756..24430
                     /locus_tag="PC460_001843"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF2600730.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            24679..25518
                     /locus_tag="PC460_001844"
     CDS             24679..25518
                     /locus_tag="PC460_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2600731.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            25762..25858
                     /locus_tag="PC460_001845"
     tRNA            25762..25858
                     /locus_tag="PC460_001845"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25796..25798,aa:Sec,seq:tca)
     gene            26013..26720
                     /locus_tag="PC460_001846"
     CDS             26013..26720
                     /locus_tag="PC460_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF2600732.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(26862..27698)
                     /locus_tag="PC460_001847"
     CDS             complement(26862..27698)
                     /locus_tag="PC460_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2600733.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSKAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            27886..28791
                     /locus_tag="PC460_001848"
     CDS             27886..28791
                     /locus_tag="PC460_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF2600734.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            28800..29984
                     /locus_tag="PC460_001849"
     CDS             28800..29984
                     /locus_tag="PC460_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2600735.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            30099..31400
                     /locus_tag="PC460_001850"
     CDS             30099..31400
                     /locus_tag="PC460_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2600736.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            31354..32397
                     /gene="holA"
                     /locus_tag="PC460_001851"
     CDS             31354..32397
                     /gene="holA"
                     /locus_tag="PC460_001851"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2600737.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(32584..32850)
                     /gene="rpsT"
                     /locus_tag="PC460_001852"
     CDS             complement(32584..32850)
                     /gene="rpsT"
                     /locus_tag="PC460_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF2600738.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT        13209 a         3375 c         5591 g        10853 t
ORIGIN      
        1 aatttctagc attataagac tataaattga aaatattcag aaaaatacta caagaaacga
       61 tttattttgc tcttaaaata agttaaatga atgttattaa ttttgtaaat tgaaatagtt
      121 taagtataaa gttatgatat aatctaataa gggtaattct ttagattata ttaaggaaat
      181 ataggtatac aataaaaagt tatggagttg gattatggga tataaattaa ttgtaacaga
      241 tatggatgga actttattag gcaataatca taaagttaca gatgaaaata aaactgcact
      301 acaaaaagtt ataaaaagcg gtattaatgt aactttagca acaggaagag ccttcgattc
      361 agctaaatgc aatgtagatt ttcttaaaga ggacatgcca ataatagctt gtaatggttc
      421 attaataaga gagcaaaatg gaaatataat atattcaaat aagattgata caagaacatg
      481 tttaaacatt ttagatgtat tagataaata cgatatttac taccaatgta atagtataga
      541 ttctatgctt tcgaaaaaaa tagaaggacg tgaagataga ttaagtgtat ttttaggctc
      601 tgaaacagaa gtaattgtaa aagatgattt gagagaagaa atctttaaaa aagatatact
      661 aaaatttgta atcatagaag aaaaaaatcc atctatttta gatgaaataa gaaaagaatt
      721 gagaaaagtt caaggaataa agataacttc ttcatggcca aacaatattg aagtcatgaa
      781 tgaaggtgtt gataaaggta atgcagttaa aattttagct gaaaaaatga atatagacag
      841 agaagatata attgcatttg gagacaatta caatgacata gaaatgataa agtttgctgg
      901 tcttggggta gctatgggaa atgcagaaga attgattaaa caagaagctg attatgttac
      961 agatacaaat caagacagtg gagttgcaaa ggcaatttat aaattcctag agttaaaaga
     1021 atcacttagc tcttaaaaca gtatataata ggaaaaaata ggttgactat tttgtagata
     1081 gtaattataa tacaaataga taaaacataa aaatttagat tcagtgaaaa agagagtaga
     1141 tatagagaaa atctcagcga attgggggat ggtgtgagcc taatgataac actttatatt
     1201 gaaaagaact ttggagaata ctacttgaaa ttcagtatgg tagtaggtaa tctcgcctta
     1261 agagaataga gtgggtaaac attgtttacc aactagagtg gtaacacgga atatttcgtc
     1321 tcttggtctt tttgaccaag agatttttta gtttcattga gttacaagac ttataattaa
     1381 aatacaaaaa attaaaatat gaggtgaaag ttatgaaatt atggggcgga cgctttagaa
     1441 aagcggaaaa tcaacttatg gaagaattta ataaatcttt tggctatgat tgtgtactgt
     1501 acaaaaaaga tatcgaagga agtgtagcac atgttcatat gcaagtaaaa tgtggacttt
     1561 taacagaaga agaaggaaag tctataacag aaggtttaaa agggatactt gaagatgtag
     1621 aaaatggaaa gttagtattg gatggagagt atgaagatat acatagtttt actgagataa
     1681 atttaattca aagaattgga gatgttggca agaagcttca tacagcacgt agtagaaatg
     1741 accaagttgc agttgatatg agattgtatg ctaaagaaaa agcaaatgat ttggtgggtt
     1801 taatatctga atttaaagct actataaaag atgttgctga caaaaaccca gtgatgatgc
     1861 caggctatac tcatcttcaa agagctcaag tagtgacatt taagcatcac ttaatggcat
     1921 attatagtat gtttgataga gatgagaaaa gaataaaaaa tgctatagaa atattagatg
     1981 aatcaccttt aggctgtgga gctttagcag gaactacgca tgatatagat agaagtataa
     2041 cttgtgaaca attaggattt aaaaaagtag tagataattt tatggatgga gtaagtgata
     2101 gagattattt actagagcta atgagtgatt tttcaattat aatgatgcac ttaagtagat
     2161 taagtgaaga gttgatactt tggagttctc aagaatttgg atttgttgag atagatgatt
     2221 tatatacaac tggaagtagt ataatgcctc agaagaaaaa tccagatggt gcagagctta
     2281 taagaggtaa gactggcaga gtatatggaa acttatttgg tctttttaca gtaatgaaag
     2341 gaataccatt agcatataat aaagacatgc aagaagataa agaaggtttt ttcgatagtg
     2401 tacatactct tgaaatgtgt atacaaataa tggatagaat gatagccaca ctaaaggtaa
     2461 atgaggataa aatgaaacaa gcagtaaaaa atggtttttt aaatgcaaca gaagttgcgg
     2521 attatttggt taaaaataat gttgctttta gagatgcaca tggtatagta ggaagtatcg
     2581 ttatatattg tgaagataat aaaaaagcaa tagaagattt aacattagaa gaattacata
     2641 agttttcaga tgcttttaag gaagatattt atgattttat cgactatgag agtatattaa
     2701 ataagggaat aaaaaagaat ttaaaataat gtatttattt ttaaaagctg taaatttgaa
     2761 tgttatcatt taaatttaca gctttttatt gtgtaagatt ttattatata aaagtaagaa
     2821 acttaaaagt agtagtattt aaaataaata caaataaaaa attgttatta aaatgataat
     2881 taatatcaat aagtattgac aaaattattt ctaaatacta ttatatataa tataaataat
     2941 aatgaaaatc aatatcaaat aaatgggagg aaatctaatg aacttattag tgattggtgg
     3001 tcatgaaaga atggaaaaag attatatggt tatggccaag aaaaaaggtt ataagacaaa
     3061 ggtatatact accatgtcat ctaaattaaa taattctata ggaagaccag atgcagttgt
     3121 catacttaca tcaactgttt cacataagat gtctagaacg gttgagtctc aagctaaaaa
     3181 acaagatata ttaatcgtaa ggcataaaaa tagtagtaag gtagcattta atgaatgtct
     3241 agatatggtt gatgaatgtc tgggaaattg ttcaaaatgt aagcttaaag acaaacaaaa
     3301 acaaaaaaat atataggtat aaaaaataca taaacataaa attaaaagta ctgagataat
     3361 agttataatt attatctcag tacttttaga tttataagga taagtatatt ataaattttt
     3421 tattataatt tccaactact tgttactttt tcatattttt tttacttcta gctttttttc
     3481 tttttttctt ctttatattt gaagctatta taaataaaac taaaagcact agaatacctg
     3541 caataatctt aacaaaagtc attaaaatat tatttccagt aaaaaatgga agactgctat
     3601 caagattatt ttttgcaatt aagtctatac tgttttctag tttaccatta ttatatacat
     3661 ttaaagtacc aaccttagcc ccttttttta taggcaaatc aattttattt aactctgttt
     3721 tggtatcata attgccttga gattcatctt tttctaagaa tatcctataa ctattttttg
     3781 gttcataaat caattcatgt tgttttgtaa acaatacttt tttagtatct gtgtattttt
     3841 ctttatcaac aatagtagca cacttaaagt tatcaaatcc ataatcaatc agaatacgtg
     3901 agtctaaata taaatcagca ttagttgaat taaatacagc aacgataact cttctgccat
     3961 cttttacagc actcgataaa agacatttac cagcagcatc agtatatcca gtttttatac
     4021 catcaactat atcatatttt atatttatag gttgaccttt gtatgtaatc ttatcatgtg
     4081 atgtcaaaaa cttattagtg tttacaaagt atctctcata aggatatgct tttgtagctt
     4141 caaatttaag tgacttagtt tttacaattt gtctaaaagt atcattattc atagcttctc
     4201 tagctatgag agccatatca tgagctgttg tgtagtgatt tgggtctggt aatccatgag
     4261 gattattaaa atgagtatta gtagctccaa ttttttttgc ctcagagttc ataaggtcaa
     4321 taaatttttg tacattgcca ccaccaacat atctagctaa tacaaaagca gcatcattag
     4381 cagaatgaac caaaagagca tctagtaact gctttacagt aaaagattcc ccatttttta
     4441 gatacatact agagccatct atttgaggta aatctttaat ttctattact tgatttaaat
     4501 ctttaacctc ctttagaact aaacaagctg tccatacttt agttgttgaa gctggataaa
     4561 gcttagaatt tccatcctta ttatatagta ttttgccaga ctcatagtcc attaaaactg
     4621 catgtttagc aactatagct ggttcattat ctgcaaaaga gatattagag cttcctaaaa
     4681 atgaagtaaa tatcaataaa cagattaata aaagtgataa atttcttttc attgtgagcc
     4741 tccaaatttt aaattcatct tttagtatag catatatttg tcaataatta aaagaaagga
     4801 gtgattaata tatgaaaaaa aaagtaaaat ttatttcgtt ttttgtttta tttattttaa
     4861 taatatcatc aataatcgtt tataaatcgc ttattttcaa agatgatatt tatgttgtaa
     4921 gtaaaaaaaa tttacctaat gaaaaaacac atgttgtaga gaatgaaatt aaaggtgaag
     4981 ataataatca caataaagta actaaagaca tagataaaaa ttttcaaaat aataaaataa
     5041 ctatatacat aagtggagca gtaaatagac ctggaattgt aactatagag tcagacaaga
     5101 gactttatga tgctgtagaa ttactaggag gaacgacaaa agaagcagat ttaaatggag
     5161 tcaatctatc agtaagatta gaagatgaac aacattacat aatacccaaa ataggtgaag
     5221 caacatctgt tacaagtaat gatgattcca ataaaccaaa tcagaggaat gagtcaagag
     5281 gtgagcctaa aaataaatcc aataaaaata tatctaacga atcaaaagta aatataaatg
     5341 tagcgactat agaagagttg gattctctac ctggggttgg agaagctaca gccaataaaa
     5401 ttttacaaca cagagaagaa aatggacagt tcagttcaat tgaggaaata aaaaatgtaa
     5461 atggtattgg agataaaaag tatgaaaata taaaagattt aatttgtgta gactaaaata
     5521 taatacaaaa cttacatata gatatgttat tataaatatg tatgtatatg tgtgtacatg
     5581 tggaaattta gtaaaggagg cacttttatg aaaaaagaac aaaaaaaact tacagagatg
     5641 acaacagcag gtggttgagc cgctaaaata gggccagagg ttctggcatc agttttatct
     5701 caattgccta aaaatgataa tatagaaaat ttattagtag gactagatac agcagatgat
     5761 gctgcagttt ataaattaaa tgatgatatg gcacttatcc aaactttaga cttctttaca
     5821 ccaatggtag atgacccata cgtatttggt caaattgctg catcaaattc actatcagat
     5881 gtatatgcta tgggaggcaa acctttggtt gctatgaata ttgtttgttt tccatcatgc
     5941 catgatatgg atgtacttgc agaaatatta aaaggtgggt ttgataaagt gaaagaaagt
     6001 ggagcacttt tggttggagg gcatactgta gatgacaaag agcctaaata tggtctttca
     6061 gtttctggga ttgtaagtcc aaataaagtt ttatcaaatg ctactgcaaa acctggtgat
     6121 aagttaatta taacaaaacc aattggagtg ggtgtattaa atacagccat gaaagaaggt
     6181 atggtagaac aacatatagc agataaagta atagaaataa tgatacattt aaataaatat
     6241 gcagcaatga gttttgagaa atttgatgta aattcggtta cagatataac tggttttggt
     6301 ctacttggtc atactttaga aatggccaaa gcttctgaag taagtattga aattgaatca
     6361 aaacatgtac caattattga aggtgcaata gaaatggcac aaatgggaat tatacctgct
     6421 ggtatgtata aaaatatgca ttatgtatct aaagatgtag aagttgtagg aaatatagaa
     6481 gttgcagttc aagatatttt atacgaccca caaacatcag gagggttgtt aatttcagta
     6541 aaagaagaac ttgcagagga acttgtaaaa gatatgaaat taaatggagc aatagaagct
     6601 aaaattatag gttctgtagt tccaaaaggg gaaaaatata ttacagtttt ataaaatatt
     6661 atagagaaag agtgattgtt ttgagcaaaa gagaattgtt tgcaatgttg ccttcagttg
     6721 atgaagtatt aagtgataat agaattgtag aaataataaa tgaatatcct agaagtttag
     6781 ttttagaatc tgtgagagaa gtaatagatt taaaaagaca atttatatta agattaaaag
     6841 aagatgcatc aaactctgta acaatagagt ttggtgaaat aatagaatct gcaatagaga
     6901 gagttaagtt aaattactca ttatctttaa aaaaagttat aaatgcaact ggtacagtta
     6961 ttcatacaaa tttaggaagg tctttactta gtgaagatat aaaagatgaa ttatggtgtg
     7021 ctgcttcaag gtattcaaac ttagaatacg atttggataa tggagaaaga ggttcaagat
     7081 actctcattt aactagtaca ataaaaagac ttactggagc agaagatgta ctagttgtaa
     7141 ataataatgc agcggcagta cttcttgtac ttagcacaat ggctaaagga ggagaagcaa
     7201 ttgtatctcg tggagaatta gttgaagtag gtggttcatt taggattcca agtataatgg
     7261 ctcttagtgg tgcagaatta gttgaagttg gttcaactaa taaaactcat ttgaaagatt
     7321 acaaggaggc cattacagaa gatacaaatg tattgatgaa ggttcataca agtaattata
     7381 gaataatggg attcacagag agtgtttcta tagaagaatt ggttaattta ggtaaaaaat
     7441 ataaactacc tgtaatagaa gacttaggaa gtggagtttt tatagattta tcaaaatatg
     7501 gtttatctta tgaaccaact gtattggatt ctattcgaca aggtgcagat gtagtaactt
     7561 ttagtggaga taagatgctt ggaggtccgc aagcaggtat tatagttggg aaaaaagagt
     7621 atatagaaaa aatgaagaaa aatcaactta ctagagcact tagagttgat aagcttacaa
     7681 tatgtgctct tgaagcaaca ttgagaatgt atttagatga gacaaaagct atagaaaata
     7741 tacctacttt aaaaatgttg acatataaaa ttgaagaatt agaagtaaaa gcaaataaac
     7801 tatttgaaaa aataactgct ttaaacttaa atgcaaacat aaatatagaa gatggatttt
     7861 cacaggtggg aggagggtct atgcctcttg aaactataag tacaaaggta atatcaataa
     7921 ctcctgaaca tatgaatgtt tcatctttag aaaaaaaact aagacttagt gaagcacata
     7981 taatagccag agtgtatgat aataaatatg tactagatgt aagaactata tttgatgatg
     8041 aatttgatgt aatagttgag gaattgagaa aagcatttaa ttaaataaat ttgagttaat
     8101 aaatattaaa tttagaagta taagtatgaa gttttgatta aaaaatagct acacacactt
     8161 gtgactttag tcatagcagg tttagagggg gcaaaaaatg aaaaatgtag taattggaac
     8221 agcaggtcat attgaccacg gaaaaactac tcttataaaa gcattgactg gaagagagac
     8281 tgatacgtta gatgaagaaa aaaagagagg tatttcaata aacttaggat ttacttattt
     8341 tgatttacca aacaatagaa gagctggaat tgttgatgtc ccaggacacg aaaaatttat
     8401 aaaaaacatg ttagctggag catcaggaat tgatatagtt ttacttgttg tagcagcaga
     8461 tgaaggggtt atgccacaga cagttgagca tttagatatt ctaagctttt taaatatcaa
     8521 aaatggaata gtggttctaa ctaagtctga tactgtagat gatgaattta gagagctagt
     8581 aaaagaagat atttgtgaaa aaataaaagg aacttttctt gaagatgctg aaataattga
     8641 agttgattct atatctaaaa gaggtataga cactcttatt gaaaaaatag ataaaatgag
     8701 tgatgatata gaagataaaa atgaaaactc tccagcaaga cttaatatag acagagtatt
     8761 ttcaataaaa gggtttggta ctgtaataac aggcacttta atagaaggaa agataagtat
     8821 agaagatgat ttggttatat atccacaggt attaaaaaca aaaataagaa gtatacaagt
     8881 tcatggagaa aataaagata cagcttatgc aggtcaaaga acagcaatca atatatcaaa
     8941 tgtaaaagtg gatgatataa aaagaggaga tgttttagct gcaccaaact cattggagga
     9001 atcaatgatg ttagatgtaa aattatcatt ggtaaatcat ttagataaag gattaaagca
     9061 ctgggataga ttaagattgt atcatggaac tagagagata ctttgtagag cagttccact
     9121 agataaagaa ttaattgaaa gtggagaaag tggctatgtt cagttgagac ttgaagaaag
     9181 catagtatct aagaaaggtg atacgtttgt agtgagacgt tattctccaa tggaaactat
     9241 aggtggaggt gtaataattg acccatctcc taaaaaacat aagaaatttg atgaaaaagt
     9301 tatagaagca ttaaaaataa aagaaaaagg cgaactaaaa gatataatag aagagtactt
     9361 aaaaaggaat ttaaaaaatt atccaaatat aaaagaaata atgagctata gtggtgctca
     9421 tgaagaagat gtaaaaagag cgcttgaaac acttatatca gaagataaag tatttattat
     9481 aggaaatatg tatatgcata taaatcaata caataaatta aaagaaaata caataaagct
     9541 tttaagtgag taccataaga agtataggct tagaaaaggg atactaaaag aagaagtaag
     9601 gtctaagata gagagtaatt ttaagactag ggagatggat attttattag aaaaattgtc
     9661 tacagaaaat gctataaaaa tagaaaataa tatagtatct ctattagatt ttgaggttat
     9721 tttaaatgat aagcaaaaag aaatagcaaa aaaaatagaa aaaagattaa aatcatgtgg
     9781 agtttcttct atactgacaa tagatgaagt ttctgaagga aatcataact atgctgaagt
     9841 attggagtca atgataggta ataaagtaga gaaattagat gatttgtata taatggacaa
     9901 agatatttat gaaaatgcaa agaacatttt gataaattat ataaaagaaa ataaagaaat
     9961 aacattagga gaatacagag atttgattga ttctagcaga aagaattgca tgattatatt
    10021 ggaaaacttc gatagaaaca aaataactaa aagagtagaa aacaaaagaa ttttgtttta
    10081 gcagaaaatg tctgtttttg ctttaaggta taaagaagga attataatgt cgagtatcgt
    10141 ataattaatt agtataaaat attaattaag aggaagtgta aagtattgaa gaatataata
    10201 cctgatgtaa aaccagatat aaaagaaaga gaagacatga gccttttatt tataataatg
    10261 ggctcagtac ttttattatt tattataaaa gatatagaga aatatgaaac tttattacat
    10321 gccatatata cgatattttt agtagctaca ggagtaatta tatttaatac tacaaaattt
    10381 aaagtagatt cattttctac atttttagga ttaattttta tagctactgg agtattggag
    10441 tgtatatatt tatttaattc cttggggatt aaaactaagt ctataatgga gattaacatc
    10501 acaatatcag caataacaga tttatttcct atactgggtg tatacttaag ttttaaattt
    10561 gttaaggata acaaacaaat ttattcaagt gtaattttat ttatagcaac tgctacatta
    10621 acaataacat ctttattcat aatatcagga atttgtgact atctgggaaa tggtaaatca
    10681 caatatgctt taggagttat aatatctata ttcataataa ttatttctat cattagtgga
    10741 attgaattaa attcaagttc aaataaaggt aaatgggaat atgatgaaaa aaagattttt
    10801 aacagaataa tagtaataat aatcttatca agagttccaa atctattgca tatagttatt
    10861 gataatagaa atgtagagag gatattatca caaataataa taaatgtagc cttatattat
    10921 ttatataatt acatagtatc taagaatatt aagaaaacag cactggaatt gcatgatacg
    10981 aatgaagaat taacaaaaaa aacggaaagt ttaaaagaaa agaataaaaa attaatctat
    11041 gagacagaaa agatagagga acttaaggga atacttatga aaagagaatc taggctacaa
    11101 tctacattag atgtagcagt aaatagcata gtagtattta gcaaagatgg agaaattaca
    11161 tatgcaaata agagctttag aaatactttt ggagactata aagaacaaga taaattgact
    11221 gataaaataa aaaacttcaa tgatttgata gaaagcattc attatgtgtt tgttcatgag
    11281 aagaatgcag agaaacttat tagtacaagc gataataaag tttatcaagc tatatttgca
    11341 cctttaatta ttgcttcaca aacagaagga gtattgtgtg tactagaaga taagactaag
    11401 aaaaaggagt acgaacgcaa acttattgaa gcaaataaga gaagtaaaga ttttttagaa
    11461 agtattggag atggcatagt agtgcttgaa ggtaataaca aaatatatgt taataatgca
    11521 tgtagggaaa tttttaaaaa taatttagag agtattgatt tttcattagt atgtaaaagt
    11581 gaagaaagta tagaaaaaag atatgtaata gatggtgaag taaagtatgt agagatgagt
    11641 ttttctcagt atactaatgc tggtactaat aaaactatta ttgtaatacg tgatacaacc
    11701 tctcgaaaaa tatctcaaat aaaacttgag gaaagtcaat catcatattc aagatttata
    11761 gatatattac ctgatggcat atgcttatta aaagaagatt taagtataaa ctatgcaaat
    11821 aagtcgtttt tagatatgct agcttttaca aacatagatg atataaaaga ttctaatatt
    11881 aaactattaa tgaatgcgaa tactgaagaa aaaatgaaat ttacggataa tatggctaaa
    11941 gttttaaatg aaaatagaag tatactttta ctggaatatg aattgataaa tagttatgat
    12001 gatatagtag aagtagaatt aagtgcatta ccttttgcta tatacaatac tagatacata
    12061 atgcttatta taaaagattt agtacataag aaatattccg agcaagcaga aaaagaactg
    12121 cttgaaagat ttaaaacaga taaaatcaaa acggagtttt ttgcaaatat gtctcatgaa
    12181 ctaaaaactc cactaaatgt tatatctagt tcaaatcaac tggtagattc tttctataga
    12241 aatgaaaaga taaaagatta caataacaat attaaatctc atgtggattt agttagacaa
    12301 agttcttata ggcttcaaag attaataaat aacattatag atttaacaaa gatggagtct
    12361 gggttctata cactaaaact tgcgaaatac aatattgtat ctgtaataga agacttattt
    12421 atgaatatag aagagtatgc acttagaaaa gacataaaaa ttctttttga tacagacctt
    12481 gaagaaatta atgtttatat agacaaggtt gaaattgaga gaattatgtt gaatctattg
    12541 tcaaactgta ttaagtttac tgataatgga ggctggatat atgtaagtat tcattataaa
    12601 atagataaag taatcataag tgtaaaagac actggagttg gaataccaca agataaatta
    12661 gagttgattt ttgaagaatt ttcacaagtt gataagactt tatccagaaa tactgaagga
    12721 agtggaatag ggcttgctat agttaagaat ctagtaagtc ttcatggtgg agatatagaa
    12781 gtagtaagtg aagtgaataa aggcacagag ttcttaattt cacttccaat gaagggattt
    12841 agtagtgaac attacacaga agataagaga atatataata ttcaagaaaa aataaaaata
    12901 gaattttctg atatatatta ttaaaaatga ttatgagcaa aaataaaata aaaatactat
    12961 ttgtttaaat atttaagttg taagaaatag gctatcataa ggtaaattat tatgatagcc
    13021 tatttttagc taggttttat atctatatgt gagaacatat aatctctaaa ttaccttcaa
    13081 aactaaaatc tccaaagtcg tttggtagta taaaatgcat accctttttt aaattgtatt
    13141 ctacagaatt gtgtataagt tttccatttc catccaacac actgtataaa gaaaaaggtg
    13201 tattgtgtgt aaaattacat tttccaaaaa catctaattt ataaacacta aaaaattcgt
    13261 ttgacacaaa tgttgtgcat ttaaagtctg atgttgatac gattttatag tcagtatcga
    13321 aatttatatg aggaacattc gtaacatcta ttgatttttg aacatgaagt tcacgcttat
    13381 tacctgaatt gtcagttcta tcataatcat agactctata agtagtatct gagttttgtt
    13441 gagtttctaa aatcaatgtt cctttgcata tagcatgtat agtgccactt ggaacataga
    13501 aaaagtctcc cttttttata ttaacctttc taagtagttt gtcccattca ttattattta
    13561 ccatatttac aaattcttta tgagattttg cattatgacc tattataatt tctgcatcat
    13621 cagaacaatc tattacatac caacactctg tcttacctaa ctctccattt tcattttttt
    13681 tagcatattc atcatttgga tgaacttgaa ctgataaatt atcatttgca tccaatattt
    13741 ttgtaagtaa aggaaactta tcaccaggtg tgtttccaaa taattctcta tttttattcc
    13801 acaattcaga tagtttttta cctttatatt ttccattttc tattaagcaa tctccatttt
    13861 tatgagagct tatagcccaa cattcaccag ttgttggcga atctatttca taattaaatt
    13921 tatctttaag tgcagtacca ccccatattc tatccataaa aataggtttt aaaaataatg
    13981 gttccataat cctgttcctc ctatgatatt aaatttatcc tacaaataat tatatataaa
    14041 tagattataa aatcaatagt aaaaaagttt tgtcccttga aatgacataa agtataagga
    14101 gttattaaat aagataaact atgatatata atataaagtt ctaaattgga gtgataatta
    14161 tggagttgtt taaggcagaa agcaaaaaat ataaaaaaga atttttaaaa ttattggagc
    14221 aatgggttag tataccttca ttttatgata gaaaaactgt atctaaagac atgccttttg
    14281 gaaaaggagt atatgatgca ttaaactggt ttgaaaatct aggtagagaa aacaatttca
    14341 aagtaaaaaa tatagataat catgctgttc aaattgaata tggaaatggt aaagagtatg
    14401 tagatatatt tggacattgt gatgttgtaa atccaggtga aggatgggat agtgaaccat
    14461 ttaaactaaa tattataggt gataaattag ttgctagagg tgtatctgat aataaagggc
    14521 ctatgatagt gaatttttta gcactaaaaa tgataaagga tttagatatt aatttaaaaa
    14581 gaaaggttag attaatagct gggggaaatg aagagagtgg atttaaatgt ataaagcatt
    14641 attatagcaa agagccatat ggtgtatgtg gatttactcc agatgctaaa tttcctgtat
    14701 taaatggaga aaaaggtgga gcaataataa agttaatttc aaatatagac gataaatctt
    14761 tatatataag tggtggaata gagtttaata caataccaga taaagtttat ataaagaatg
    14821 tagaaaaatt gggtaaagat aatatatgtt ttgatattaa caatatatcg ataaattatg
    14881 ataatggcaa ttatattgtg tatggaaaag gtggtcattc atctaaaccc gaaaaatcaa
    14941 taaatcctat attagctaca attaaacttt tgtcagaaaa tattgatgaa aaatggacaa
    15001 aagatttata taagttgata aatcaagata atataaatgg aaacttattt ggtttaaata
    15061 ttgaggggaa atgtggaatt ttatcaatgg tgccaacaat aataaatata gtagatggaa
    15121 aattagaagt agttttaagc gttagatatc cagaaatatt aaccattgaa gatataataa
    15181 aaaaatttaa tttgtatatg gagcaaaata atattaataa atttgagctt attggggaaa
    15241 atttaaagca agcaaattat atagatagaa attcaaaact tgtaagaagt ttgcatgata
    15301 tatatataaa gtattctgga gatttaaaaa atgatgttag agtaactagt gcaggaagtt
    15361 atgcttctga aatgaataat tcagtaatat ttggttgtga atttcctgat ggtagctttg
    15421 gcaatgtcca tagtgcgaat gaatttgcta gcttagatag atttataact gcaataggta
    15481 tatatgcaga ggcaataatt acattatgta ataaaatata aatacaaaat gtcccttata
    15541 tagacatatt atatatacaa ttaaaatttt acttgatata taattgaaat caaagagaag
    15601 gtgatacaat gataactaag aaacatgcaa taatagtaaa gagtttaagc aataaagatg
    15661 gatatatgac atcaaacgaa ttagctatca aattggatgt gtctactaaa actattaaaa
    15721 gatatatagc agatttaaat agtgttttaa gcaaatatga tttggtaata gcttcttcta
    15781 gaggtatagg atacaagtta agcggttcta aaaacaatat agccagagct gttaaagaag
    15841 caaataaata tattgatggt tttttagatg actctgaaga gtcaagaatg tcaaatataa
    15901 tatgtatgct tattaataga aattatatga gtatagaagc aatggcagaa gaattaaatc
    15961 taagtatagc cgctataaat aaattgtcta gtaagcttaa aagaaaactt gaaaagtatg
    16021 acttagttat aaaatctaaa ccttactatg gaagctatat acatggtgaa gaaattaata
    16081 taaggcaatt aattactgac tatgcaataa aattagatga aaaaagtaaa gtgaaggtgt
    16141 ttttagatga cattagtgaa aacgatatac actgtataga aagtatttta gaaaagcact
    16201 taagagaaaa agatacaata atttcagata aagattttaa tcttttgctt tcaaaaataa
    16261 tcgtttctgt atttaggagt aagagaggtc atagtaacaa tataaactta atggatacaa
    16321 gttatagatt tcataactac tactttatag aaaatctaat gaaagaaata tctgataaaa
    16381 ttggatttaa acttattgaa gatgaagtaa tttatatttc aaattattct ggtgtaatag
    16441 cctataaagg tacacaagga agaaaaaaca caagtgaaat tgaggaacga ataagtactg
    16501 ttataagtag tgcacttcaa gatatattcc ttatttcagg tagtgattat acaaaagatg
    16561 atgagtttat ggttgcaatc tctgaccata taaagagatt tttaaataga gctagagcta
    16621 atgtaaaatc aaataatcct ttacttcatc aaataaaaga gaagtttcct atagctttta
    16681 atttagccgt gtttttatca aataaactag aaactgagtt taatttaaaa cttgatgaag
    16741 atgagcttgg atatatagct atccactttg ctgcttcaaa tgaaagaatg aaaaaaaata
    16801 ctagtaagaa aatttgtata gtttgtcatt atgggatagg aacaggacaa cttatttctg
    16861 aaaagctaaa acagaatata agtgatttaa gtgttgtggg tgtttatcca gttagatatc
    16921 tcgatatggc tataagtcaa gatgttgatt tgatagtatc tacagttgaa ttaaaaggat
    16981 atgaaaaacc tgtcttatat atagaaaata tatttgatga tagcttaatt gaaaacgtaa
    17041 acaaagcctt ttatgaaaag gaagaaagaa gaaagataat aagtaacatg tttgatgaaa
    17101 aagctttttt cagtataaag gctagtacta aagaagaggt cataatttca ctttcaaata
    17161 aactaaaaga aagagatttt atagaggaaa gttctataaa aagtataata gatagagaaa
    17221 atatttcatc aactgaaata ggaaatctag tagcaatacc ccacacaata gtgaagggag
    17281 ataaaaaatc tataatagga gttggaatac ttgaaaatcc aataatttgg gataagcaag
    17341 aggttcagct agtatttatg gtatttttta atactaaaga aaaacataat ttctcgatat
    17401 ttaaatacct ttataatttt ataaaagatg aaggaggagt aagaggaatt attaaaatat
    17461 gtgactttaa taaattaatg gcacttatag gtaattaagg aggaaattaa taatgggaac
    17521 aaacatattt aacaaagaat atgtattttt aaatgttgat gctaagtcaa aggttgaagt
    17581 tttaaaattt atatcaaaaa aagctaaaga cttaaacttg gcagaagatg agagcttagt
    17641 atatgaaggt ttaatggcta gagaagaaca atttactaca aatttaggag aatctatagc
    17701 aatacctcac actaaaaatg atgcaattga aaatccagca gtagtagtat tgaagtttaa
    17761 tgaagatgtt gtttggaatg aaggtgagga taaggttaaa ttagcaataa gcttacttat
    17821 gccaggaaaa agtaaagaga atattcatct taaattatta tcatctcttt caagaaaatt
    17881 aataaataaa gaatttaaag attctctttt aaaaagtgat aatgttgaag aaatatctaa
    17941 cttaataaat gaagctttag gtttataatt aaacactaag gttataaaat aaaaaaataa
    18001 ttaactaaaa ataggaggaa tttgaaatga ataaaaaatt agtagcatta tgtgcttgtc
    18061 caatgggact agctcacact ttcatggcag cagaagcaat agagcaagca gcaaaggcat
    18121 taggttatga agcaaaagtt gaaactcaag gtgctgatgg tgtacaaaat gagttaacaa
    18181 gagatgatat attaggagca actatgataa tacatgcagt agcaataaca ccagaaggta
    18241 tggaaagatt tgatggttgt gaagtttatg aagttgaatt acaagaagct ataaaaaatg
    18301 cagaaggtgt tataaaagaa atagaagaag atttaggaat ataataaatt tattgtggag
    18361 gaaatagaat ggcaattaag aaaaaagtta taaacagttc aggtgcttct acaacaggag
    18421 gaaataatgt aggaaaatct actccatcta gtccaactca atcaaaggga aatggaaaat
    18481 ggaaagaaat aagtaaacat ataatgacag gtatatcata tatgatacca gttttagtaa
    18541 tgggtggact tataggggcc ttatctcaat taataccata tgctatatta ggacttgacc
    18601 catctgtagg tatagttgat gctatgaact caggagaatt tacagggttc aagcttagtc
    18661 ttttaaatat agcacaatta atgtcaaatt tcggatttac tttgtttgga tttgcgatac
    18721 cactgtttgc agcattttgt gcaaactcta taggtggaaa aacagcttta attgcaggat
    18781 ttataggtgg atatatagct aataaacctg taggtgtacc acaattcgtt gatggacaat
    18841 ggactgaagt tgttccagtt gcatcaggat ttttaggtgc aatactaata gcatttataa
    18901 taggatattt tgtaaaatgg ttaaataaat ctataaaagt ttctcataac tggttagctt
    18961 ttaaaacaac atttttaata ccattaatag cgtcattagc ttgtatggta ttgatgatat
    19021 ttataataac tccatttggt ggattaatta atgaaagtat gaaaaacttc ttaacagcag
    19081 caggagcagc tggagaatat gtatatgcaa cagctttggc agcagctaca gcattcgact
    19141 taggaggacc aataaataaa gcagcaggat tcgttgcact tggtttaact acagagaatg
    19201 tacttccaat aacagcaaga acaatagcta tagttgttcc tccaattgga ttaggattaa
    19261 caacattatt agataaaaga ttagtaggaa gaagagtata tgatagacaa ttctatcaag
    19321 caggaaaaac ttctatattc ttatcattta tgggtatatc tgaaggagca ataccatttg
    19381 cacttgagag accaggtttt gttatacctt taaacatagt tggaagtgta ataggagcta
    19441 ttacaggtat aattctagga gcaattcaat ggttcccaga gtcagctata tgggcatggc
    19501 ctctagtaga taacttattt ggatatataa taggtatagc agttggtgct atattcatag
    19561 cagtaggaaa tatattctat agaaataaat taataaaaga tggtaaactt gttgtagatt
    19621 atatcgacta gtatttaatt tttactatct ataaataact ttggctggta atatggtttt
    19681 aatcaatatt accagcctta cctatatgat taattaaagg cggtggagat aagatatgga
    19741 taagatagaa aaagtaagag agtattttag agaatatgat atagatggat ttcttataaa
    19801 tagtcctaca aacaagtttt atgttggaaa tttatttagc tcttcaggtt atgtttttat
    19861 aactaaagaa tcacaatata taatagtaga ttttagatat tttgaagaaa ttaaaagaaa
    19921 atctagtttg tttaatgtag tattaatgga caaaactaga actcattttg atataattaa
    19981 tgatatttgt agagagcaga atataaagga aattggattt gagggaaatg aagtatcttt
    20041 tgatttatat agaagtatga gtaataagtt aagtgcaacc ttaaaatcag ttgatttatc
    20101 tacattaagg gaaacaaaaa atgaagacga aataaaatat ataaaaaaag cttgtgaaat
    20161 agttgatgct acattttatc atatagtaga ctttataaag gttggtatga ctgaaaagca
    20221 agttgaaaat gaaattgtta gaattataaa agatttaggt ggtcaaaaag aatctttcga
    20281 tacaatagtt gcatctgggc ttagaggagc attacctcat ggaaaagcaa gtgaaaaagt
    20341 tatagagtat ggagattttg ttacctttga ctttggtgct aagtataata attattgttc
    20401 agatattacc agaaccattt gtatgggtac tataaataaa gagttagaag aaatatataa
    20461 tatagtaaga aaagctaatg aagagtgtat aagggtttta agaccgggaa tgactactgg
    20521 agagatagat aaagttgcaa gagacataat tggttcatat ggatatgcca ataattttgg
    20581 tcataatctg ggtcatggtg taggaattat ggttcatgaa tatcctgcat tagctcctga
    20641 atcaaatgaa gtattaaaag aaggaatgat tgtaactata gagcctggta tatatgttcc
    20701 aagtcttgga ggcgtaagaa tagaagatga tgtacttata acacaagatg gatgtatgag
    20761 attgactaca tctactaagg atttaatagt tgtaagctaa aaataaatta gataaatatt
    20821 ttataacaat aagaaaagaa gaggtaaatt taaatgtata attttgatga taaaccagat
    20881 agagtaagtg aaaagtgtag aaaatgggat ttaaatatta taagagataa atttggagat
    20941 attagagaag attttatacc aatgtggatt gctgatatgg attttaaaat accaacagaa
    21001 atagaaaaca aatttattga agctgtaaga agaggtgtat ttggatatac atactgttat
    21061 gatgaatttt atgattcagt tattggttgg caaaaaaata tgcatgaggt aaatgtagaa
    21121 aaagaatgga ttacattaac ttatggaact gtatcaacac ttcattatgt agtacaagct
    21181 ttttgtaaga aaggtgatag tataatatta aatacaccag tttatgaccc ctttgaatca
    21241 tcagcaaaaa aacaaggtgt aaatgtaatt tgtaatacat tagatgttgt aaatgataga
    21301 tattatataa attttgataa attagaagtt caaattaaag aaaataagcc taaattaatg
    21361 atgttttgta caccacataa tccatctgga aggatatgga caattgaaga aatgacaagg
    21421 gtagcaacta tatgtaaaga aaataatgtt attttggttg cagatgaagt acatgcagag
    21481 catattcatt atggtacatt taattcaata cttaagattg gaaaagagct tttagaaaat
    21541 gttattctat tgacatcacc aaataaagga tttaatcttg gtggattaaa gacttcttat
    21601 tcaatagtta taaataagga tataagggac aaatttagaa ataaattaaa gcaaaattct
    21661 ataacttctc caaatgtgtt tggaataata ggattgataa ccgcttataa tgaatgtcat
    21721 gagtggttaa gtggtgtaaa tgaatatata aaatcaaatt atgaactttt agaaacttgg
    21781 gtaaataaat ataataagat aaaattgatg aaaatggaat catcttactt agcttggatg
    21841 gatataagtg gattaggtat tagtgcaagt gagtttacag ataaattggc tatagataca
    21901 ggagtattat tagaagatgg aagtcatttt gtaaaagatg gtgagaagta tgttagaata
    21961 aatttaggaa ctcaaaagga aaatgtaatt gaagccttaa atagaatgga tttattttta
    22021 aagtctttat aaaaatttgg ttgaattagt attttatata aatttaaact attaatatgg
    22081 tatatacata agtagaattt gttttagatt acttaagtat ataccatatt ttatataaaa
    22141 attaaatttg cattagtcat gtgataacta tttaaattca tatgtatttt gtatgtataa
    22201 tagatataat tattatttaa acttattgtt tatttaattt ttcaatagaa aaagatttat
    22261 ctgtaagttt aaaattatct tttataccct tagttaagta tactttctta tccttagtta
    22321 tgaatatagc atctacatta tcaattttat taattaaatt catacctttt tcaagaccaa
    22381 gaccaaatgt agaagtggat aaggcatcac aattaataga tttatcagag attattgtaa
    22441 cactacttaa attgttttca aaaggatacc ctgtaaatgg atttaacata tgatgatata
    22501 tttttccgtc tttttcgata aatctttcat aaattccaga agtaacaaca gacttatttg
    22561 tagtttctat gtttcctatg gattcaccat ttttactagt tgggtcttga atacctattt
    22621 taaaaggttt atcattttta ccattttcta aagtaaatat atttcctcct aagtttacta
    22681 aacctttttt tacatcctct gattttaaat aggctacaat tttatcagca gcatatcctt
    22741 tagctatggc acctaaatca attttcatat tttctttggt taattttata gatttatttt
    22801 tttcatccaa tatgatgttc ttataatcaa taagaggcag aagtttttgt atcttagaat
    22861 cacttggaac tttagcttta tctgttccaa tagcccataa atttgataat ggaccaattg
    22921 ttatgtcaaa atatccatct gataatttgg aatattgtat agcttctttt acgacgaaaa
    22981 atgtatcatc agaaactctg acaaattttt taccagcatt atcatttatt ttagaaacat
    23041 cacttcctgg tatatgagtg ctcattttgt tgtcaatata tcttaaaatt gaatcacatt
    23101 catttaagat tttatcactt ttggattctt ttacattaaa tacagtaact tcgtttactg
    23161 taccaaggta gtagctagtt ttactatatt tagtattctg gcttgaactc ctaaaaaatg
    23221 aaaataaaat taatgctaaa atcaatatca caaaaataaa tgtgattttt ttcttattca
    23281 tggtatattc ctcctatgaa attgtgttat tattttatta taacataatt tcataggagg
    23341 acaaaaaatg aagaaaaaag acattcttct tattttaggc attttggttg ttattacagc
    23401 atgttatggg ataataaatg ttataaattc taaaaatgct ggaaatattg agatttatgt
    23461 agataataaa ttgtataaaa ctgtttcaat aaatgccaaa gaagaattta aaatagagag
    23521 tagagggggg tataacatag taaagattca tgataaaggg gttgagatag tagatgcttc
    23581 atgccctgat aaggtttgcg tacatactgg atttataaac aagccaagtc aaagcatagt
    23641 ttgtatacct aatagagtga gcataaaaat aaatacaaat gaaaagaatg ataatcaaga
    23701 agatataatt tctaaataaa ataataaaaa taattgaaag gaattggtga taattatggt
    23761 taacttagga aacgattggg atgaattatt gaaagaagaa tttgaaaaag attactactt
    23821 aaacttaaga aaatttctta tagaagaata taaaacacgc caaattttcc caaatatgca
    23881 caatatatat gaagctttaa aacacacttc atacaaagac acaaaagtct taatattagg
    23941 acaagaccct tatcatggag ataatcaagc tcatggttta gctttttcag ttcaaccaca
    24001 agtgaaaact ccaccatctt tattaaatat gtataaagaa ttaaaagatg atttaggttg
    24061 ttttatacct aataatggat atttaatgcc ttgggcagac caaggagtgt tacttttaaa
    24121 tactgctctt actgttagag cgcatgaagc taattctcat aagaacaaag gatgggaaat
    24181 tttcactgat agagtaatat ctattttaag tgagcgtgaa gacccagtta tttttgtact
    24241 atggggaagt aatgcacgta aaaaagtaga gttgattgat acaagtaaac attatatttt
    24301 ggaagcacct catccaagcc cattgtcagc aagtaaaggc ttttttggat gtaaacactt
    24361 ctctaagata aatgaaattt tgaaaaagct aggaaaagaa cctattaatt ggcagataga
    24421 aaatatataa aagtaaaagg taacaaaatg ataacaaaac aacaataaac aatgacattg
    24481 ttgtaactaa tagaaatatg cttaaatagt tgaaaatact atgtatgcat atttctattt
    24541 ttttgtcaag ttgacaactc ttgaaaaaat agttacaatt tagttacaca aaaaacacaa
    24601 ataaaaaaag aaacttacaa agcagaattt tatgaagcaa aatatatagt gtaaaaaatc
    24661 ttagggagga aattttatat gaattttaaa aaattgagta atgctaaagt ctcggtaatg
    24721 gcagttgttt tatcagttgg aatattatcg ggatactctg cgcttaataa acaagtaaca
    24781 ttagtagtaa ggggagaaga aagaactata tcaacattta gttctaatgt aggagacctt
    24841 ttagaatctc aaaacattag ctatgattca aatgatatag taactttaga tgaaaataca
    24901 aagttatcta atggagataa aatagaagta attgatgtaa aggaaaaaac agttgtagaa
    24961 actaaagaca ttccatttga agttacagtt gttgaagatg gctctttatt aaagggtgat
    25021 tctaaggtta aagaagaagg aaagtctggt aaaagtgaat taatatataa agaaacttat
    25081 cacaatggaa agaaagtaga aaaggaattt gtgaaaaagg ttgttaaagc tgagccagta
    25141 aataaggttg taagcaaagg gacaaaggta gaaatgcaag tagcctcatc aaggggagaa
    25201 agttcaagaa gaatggcttc atcatcatct agtagcagta gtagtaacaa tggtaaaaat
    25261 atgagagtgg tagcaactgc atatactggt cacagtataa ctgcaacagg aacaaaacct
    25321 aagtggggaa caatagctgt agaccctaag gttataccat atggaactaa agtttatata
    25381 cctcaatttg acatggtgtt tactgcagaa gactgtggtg gagcaataaa aggaaataaa
    25441 attgatatct acatgaatga ttcaaaaaca gtttataact ggggaagaaa aactatagat
    25501 atacgtatat taaaatagtt aaataattta tatttttact ggaaattata ttttgattat
    25561 atagagaaga aactctttat agattctaaa aatataattt ccagtttttt attttggata
    25621 tgtaataaaa caggtaaatt ttaaccatgt atagataaaa gctatttaaa ttataattta
    25681 ctatcatata aatctataaa aattgttgca aagagtcaaa atatatgata ttatagattt
    25741 cgttgagaag gatttcatta tgggagtgga tgggtgccgg tgtgccctct agtcttcaaa
    25801 actagtatga ggggttaaga gcctcttagg tgggttcgat tcccacgcat tcccgccaat
    25861 atgtaaaagg tatcttttaa ttaaaagata cctttttaaa tttattttaa gataatttat
    25921 ttttaataat atataatgtt ttgtattata taatgtaaat tttctaatac aaaacattat
    25981 atatttagat aaaaatctag gagtgtggaa agatggaaaa taattttaca acgagaacta
    26041 gttttctagt tggagatgat gggataaaaa aacttaataa ttcaaatata atagtatttg
    26101 gagttggagg agttggtagt tttactgttg aagctttagc aagagctgga gtaggcaata
    26161 ttactattgt agattttgat gatgtagata taaccaatat aaatagacaa ataccagccc
    26221 tacattcaac tgtaggaaga tacaaagttg atgtcatgga agaaagaata ttagatataa
    26281 atcctaatat caatataaaa aaaataagaa gtctttataa taaagatact agtgacgaaa
    26341 tactaactga aagatatgat tatgttgtgg atgcgataga catggtaagt tccaaaatac
    26401 atctaattga aacttgtgaa aaaaaaggat tgaaaataat aagttctatg ggtatgggaa
    26461 ataaattaga cccaactaaa atagtcgtta cagatataca caaaacaagt acttgtcctc
    26521 tagctaaagt tatgaggaaa gaacttaggg atagaggtat caaaaagtta aaagtagtat
    26581 attctactga acaaccaata gaacttaaga agaaagtaat gaatggtaga aaagtaacac
    26641 ctggtagtgt atcatttgtc ccatctgtag gaggtttaat aatcgcatct gtaatagtaa
    26701 atgaactatt aggacaataa gttttaaata ttttaattta taaattttat atacaaattt
    26761 gaaggctaga agcatgaata caaagcaatt ttatattcat acttttagcc taattatttt
    26821 atattacctt attttatgta gttgtagttt tattttttag attatgtatc tatttcataa
    26881 ttatattttg taaatataat ttctccatca ctataaaaaa caatatctcc tattctatct
    26941 gttctataaa ttgatacatg gttttgtttt agattattaa taacctccct atgtggatga
    27001 ccatattgat ttttatatcc acatgatatt gcagctatat ctggactaat tttttttata
    27061 aattctaaag atgaagaaga attacttcca tgatgaccta tttttataaa attaatatta
    27121 tttaatttaa aagaatgtaa aatttctttt tcattttctt cttcagcatc acccataaat
    27181 aaaaaagaat tatcattaaa agttaatttg aaaactatag aatttaaatt agattcttct
    27241 tgtatatatg atggacttaa tacatatata tttatattgt tatctatgtt taaaacatca
    27301 tttttataga gatgctgaat agataagttt ttatccgtac aagaatttat tagattttga
    27361 taagccttac tatcagttga ttgttcaggc atatatatgg agtttacatt aaattttttt
    27421 ataatattat caagacttcc tatgtggtct gaatctggat gagttgctat tataatatca
    27481 atagttttta ttcttttttg tctaagataa ctaataatta tattttcgct atcttcatca
    27541 cctccatcaa ttaatatatt cttatttgtg ggtgtttgaa ctaaaatact atcaccttgt
    27601 ccaacatcaa tcatatgtat agatagtaga gattttttgt cacagccgat taaaagtgat
    27661 attataataa gtattgataa aaaaatttta tttctcaata aaaaatcact ccattcatta
    27721 catgtttata taaatttatg atgggtaata taagatatat atgacttatt atattttata
    27781 ttttaaacaa tataaatgaa attaatacca taataaaatt ggagtttttt atatatacaa
    27841 aataaaattt ttaaaattaa gcaataaaaa aggtaggagg attttatgtt tttcaaagat
    27901 attgaactaa attcaaaaaa agagttagac ccatattttg atttagtaga ttatgaagct
    27961 tgtgagtact gttttagtac tttatatatg tggcaacatg tttataagac aggatattat
    28021 ataggtgaag attttgccgt tttagttggg gaatacgaag gagatagctt ctcaatatta
    28081 ccacttgcta aaaaagataa acttccagaa gttgtagatt ttgtccttga atatttttct
    28141 aaaaataata aaaaaatata cttaagaggt attactactg aagtagtaga gtttttaaaa
    28201 gaaaaatatc caggtagatt tgagtatata gaagaaagag atttatttga ctatatttat
    28261 gatgcagaaa gtttaagaac tctagctggt aaaaagaatc aaaagaagag aaaccatata
    28321 aactatttct taaaagagta tgcaggtaga tatgaagcta aattattaga taaagaaaac
    28381 tttgatgagt gtttagtact aatgaaggaa tgggaaagta ataaggaaga aaataatgaa
    28441 tttgatgaaa gtatggatga tgaattaata ggtatcaaaa agatatttaa ccattatgac
    28501 atattaaaag ataaggtaaa agtgtttggt gtttatgtag atggaaaatt agaagctttt
    28561 agtataggag agcttttaaa tccaaatatg gcattgattc atatagaaaa agctaatccc
    28621 gatataagag gtctgtatcc atttataaat caacaatttt tggtaagtga atttaaggac
    28681 gttgagtttg taaatagaga agaagattta ggtattgaag gattaagaaa agctaaactt
    28741 tcttatcatc cttgcagatt tgttgaaaag tacagtgtta gggaggctta atagactata
    28801 tggagattag gtatgctaaa gaagaagaga tagaaagtat aaaagatatt tggagttatt
    28861 gttttaacga tacggagagt tttatgaagt actattttaa tgataaatat aaatctgaaa
    28921 atactgtagt agctttagat gaaggaaaaa ttatttcttc tcttcagcta aatcagtata
    28981 agttattact aaatagcaag gtatataata catcatatgt ggtaggagtt tctacacttc
    29041 cagagggaag aggagctgga tatatgaaca aagtaatgaa atttacttta aacgaattgt
    29101 ataaaaaagg gcaattagtt tctatattga tgcctattga ctatagactt tatagaagat
    29161 ttggatatga gcattgttac gaccagattg aatatactat taacacagat gatttaaaaa
    29221 attttaagtc aagtggaaaa atgataaagt ctaatttatc acaaattgat gatttgatac
    29281 aaatagatag aactttttta aatgaggtta atggtaatgt gttaaaagat gaacattatt
    29341 atgaaaactt atttaaagag attcaaagtg aagatggatt tttatacatt catgaaggaa
    29401 atgaaaaaga tggatatata gtttattttc ttcaggaaga taaaatgttt gtcagagagt
    29461 tattttataa aaatatagat gcccttaaat caatgcttaa atttatatat aatcacaata
    29521 ctcaatgtaa gatagttact atatctactc ctacaataga taagataagg tttatattag
    29581 acaaccctaa agatagtgat ataaaaataa aaccttttat gatgggaaga gttataaatg
    29641 taaagaagtt tattgaagat atagatattg agaaggatat taatagctca tttaatttat
    29701 taatagaaga caagtttata gatgaaaata atggtctatt taaaatttct atacagaata
    29761 agaaggtatc tgtagagcag ttagataaaa aaggtgcaga aaagcctcaa gaagattttg
    29821 atattaaatt agatataaat accttaacac agttatcatt ttcttatata gatgtaaatg
    29881 aagctatttt cttaaatgat ataaaagatg tttcagaaga aacactagaa acacttaatt
    29941 gtattttttc taaaaaaaat aactatataa atgaatatat ataggtagag tacgttaaaa
    30001 gattctaaaa atcttttgac gtattttttt gtttagataa caatttatct tgtttaaaaa
    30061 ttttctattt gaaaataatc taaataatat acttttatat gggagtgatt ataattcaaa
    30121 taagaagacc attattaatt atattaatgt ttgtggtagc tatatcattt atcattacaa
    30181 ataacacaaa aaacgacgaa gaacttaatg ataagataat aactatacaa ggagttgtca
    30241 aaggaaaaat tgaaaagaaa agatacaatc agtacaaggt aggggttttt ctaataaatg
    30301 attatacaaa aaataaaaat ttgaagattg gtcagaaagt taatattaca ggtaaattca
    30361 aaagtttaga taaaatgaag tatgatgatt ttgattatgg aaggtatata aaaagtactg
    30421 gatataaagg tatagtgtat ataaataact ataaaattat tgataaaaat aaactttact
    30481 ctttaatagg ggaaataaag ttttatatta gtaaaactta taggtactta tataagaaaa
    30541 actcagattt tataaattct atactattag cagaagttga aaatttgact gaggaacaaa
    30601 aagaaatttt taccagaact ggaactagtc atgtaatatc tatatcaggt cttcatacag
    30661 gaatattatg tgtaattata tcctttctat taagaggtat aaataaattg tataaattat
    30721 taatactgtc gatttttata actttatatt gtataatggt aggtgcgtct ccatccataa
    30781 ttaggtctat agcatttgtt atgatttttt atttatcaat atttattgat agaaagaaag
    30841 atggaatatc ggcattgtct ctaattggga taattttaat tatgaataac ccctatgtta
    30901 tatataatgt aagttttcaa ttgtcttttt tagctacact ttctatatta atattctata
    30961 ataaaataaa tagtattata aaattaagta tggtatcatt gacaatatca tcaaatatat
    31021 taactttacc tataatttat tatacattta aaggtatacc attgctttca attataggaa
    31081 atttaattat agtgccattt gtgggagtaa taatgtattt aagtattgca agtttaatcg
    31141 tatttaaagt aagtgtagta atagctaaaa ttatatcttt ttttaatagt acatttatag
    31201 aaagcatatt ttttttacta gaaaaaatca gcaatttaag ttttgcatac attaatatag
    31261 aaaatcctaa attttatatt gttgttattt attacatagg agtgtttttt tacatatttt
    31321 acatagaagg aaaagagata aaggagcaag agaatgaatt acaaggatat tataaggaat
    31381 gtaaaagaga aaaactttga aaaaatgtat cttttttatg gaagagagta ttatttaatt
    31441 gagaatgcaa taaaagcatt taaagatagt ctaaacgagg gaatgcttga ttttaattta
    31501 gatataatag atgggaagga aatagtctta aatcagctta taagctctat agaaacactt
    31561 ccatttatgg atgataggaa gatagtaata ataaaagact ttgaactatt aaaaggaaag
    31621 aaaaagaatt ttacagatag tgatgagaaa tacttaatag agcatttaga caatattcca
    31681 gacaccacaa ctatagtttt tgttgtatat ggagatgtag acaaaagaaa atcattagtt
    31741 aagaaaatag gaaacaatgg aattgttttt gattgtgata agctttctga tatggatttg
    31801 tttaaatgga taaaaaaatc ttttgcttta aatgatgtta taatagataa ttctcaaatc
    31861 atgtatttta ttgaacaaga aggatacaga gataagagta gtgaaaagac attatctgat
    31921 ttagaaaatg aaataaataa aataagttca tttgttggta aagggaataa tgtaaccaat
    31981 gatgtaattg ataaattatc tcaaaaaaaa gttgagaatg atatattcaa attaatcgat
    32041 tatattggag aacaaaatgc ttctaatgca atgaaaatat taaatgatat gatacaagaa
    32101 ggcgaatctg tattaggtat attttctatg atagcaagac aatttaaaat aattatgcaa
    32161 gtaagacagt tacaacttga tggatattca acaaaactta tagctgataa attaaaaatg
    32221 catcaatttg ttgtaggaaa agcattaaag caaactaaaa atttttctga tgatataatt
    32281 gtggaaatat taaactatat attagaaagt gattacaaaa taaagacagg tcttataaga
    32341 gatactttag cagttgaaat gttggttagt agatactgta aaagagaagc aatttaaaaa
    32401 atctatatat taataaaaaa taaatagcaa gatatgttat agttcagagt agatgtaagc
    32461 taaattaagt ttgatataca atcaactata atatgtctaa ctatttattt tttattgtat
    32521 aaatttacaa aataaaaaac ccctacatag gggttattat atataagaaa tataaactgg
    32581 taattaagcg ttcataccgt ttaatttttg agctaattta gctacttttc tagctgcagc
    32641 attcttatgt atagttcctt tagaagctac ttggtatatt cttttttgag cgtattggaa
    32701 cttagcaaca gcatcctctc tgtttccagc tgcaacagca tcttcgaatc ttcttattgc
    32761 agtttttatt tgagattttc taactctatt taaagcagtt ttcttttcga taacacttat
    32821 tcttttctta gctgatttta tatttgccac cgtgttcacc tccctgtatt tttttatcat
    32881 attcatcgtc aacatattag attttaacag aaataaatct aaaaatcaag gattattatt
    32941 tatatatttt aataatgttt tttattttga ttttttagac tgtaaattgt gtataaataa
    33001 tttgcaatat cttgttttta aattggta
//
LOCUS       DAMJWU010000036        31710 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMJWU010000036 DAMJWU010000000
VERSION     DAMJWU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.036"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            294..638
                     /locus_tag="PC460_001853"
     CDS             294..638
                     /locus_tag="PC460_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF2600739.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPVRFDVIEVYL
                     LEKRINHIMNAF"
     gene            669..2270
                     /locus_tag="PC460_001854"
     CDS             669..2270
                     /locus_tag="PC460_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF2600740.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2289..3389
                     /gene="dprA"
                     /locus_tag="PC460_001855"
     CDS             2289..3389
                     /gene="dprA"
                     /locus_tag="PC460_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF2600741.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3422..5509
                     /gene="topA"
                     /locus_tag="PC460_001856"
     CDS             3422..5509
                     /gene="topA"
                     /locus_tag="PC460_001856"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF2600742.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5674..6459
                     /gene="codY"
                     /locus_tag="PC460_001857"
     CDS             5674..6459
                     /gene="codY"
                     /locus_tag="PC460_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF2600743.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6566..7831
                     /locus_tag="PC460_001858"
     CDS             6566..7831
                     /locus_tag="PC460_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF2600744.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDVEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDSHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7862..8632
                     /locus_tag="PC460_001859"
     CDS             7862..8632
                     /locus_tag="PC460_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2600745.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMIFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8741..9187
                     /locus_tag="PC460_001860"
     CDS             8741..9187
                     /locus_tag="PC460_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2600746.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9205..10398
                     /gene="nifS"
                     /locus_tag="PC460_001861"
     CDS             9205..10398
                     /gene="nifS"
                     /locus_tag="PC460_001861"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF2600747.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLDNRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10452..10892
                     /gene="nifU"
                     /locus_tag="PC460_001862"
     CDS             10452..10892
                     /gene="nifU"
                     /locus_tag="PC460_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF2600748.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11181..12260
                     /gene="mnmA"
                     /locus_tag="PC460_001863"
     CDS             11181..12260
                     /gene="mnmA"
                     /locus_tag="PC460_001863"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF2600749.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12403..12641
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12709..15348
                     /gene="alaS"
                     /locus_tag="PC460_001864"
     CDS             12709..15348
                     /gene="alaS"
                     /locus_tag="PC460_001864"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF2600750.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15373..15630
                     /locus_tag="PC460_001865"
     CDS             15373..15630
                     /locus_tag="PC460_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF2600751.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15640..17811
                     /locus_tag="PC460_001866"
     CDS             15640..17811
                     /locus_tag="PC460_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600752.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSCASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18017..18442
                     /gene="ruvX"
                     /locus_tag="PC460_001867"
     CDS             18017..18442
                     /gene="ruvX"
                     /locus_tag="PC460_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF2600753.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18520..18807
                     /locus_tag="PC460_001868"
     CDS             18520..18807
                     /locus_tag="PC460_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF2600754.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19089..19547
                     /locus_tag="PC460_001869"
     CDS             19089..19547
                     /locus_tag="PC460_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2600755.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19664..20401)
                     /locus_tag="PC460_001870"
     CDS             complement(19664..20401)
                     /locus_tag="PC460_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2600756.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20618..22294
                     /locus_tag="PC460_001871"
     CDS             20618..22294
                     /locus_tag="PC460_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF2600757.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22502..22702
                     /locus_tag="PC460_001872"
     CDS             22502..22702
                     /locus_tag="PC460_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF2600758.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22756..23919
                     /locus_tag="PC460_001873"
     CDS             22756..23919
                     /locus_tag="PC460_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2600759.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLT
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24130..24552)
                     /locus_tag="PC460_001874"
     CDS             complement(24130..24552)
                     /locus_tag="PC460_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF2600760.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24573..25409)
                     /locus_tag="PC460_001875"
     CDS             complement(24573..25409)
                     /locus_tag="PC460_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2600761.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIILDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25707..26303
                     /locus_tag="PC460_001876"
     CDS             25707..26303
                     /locus_tag="PC460_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF2600762.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            26333..27052
                     /locus_tag="PC460_001877"
     CDS             26333..27052
                     /locus_tag="PC460_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF2600763.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27054..27593
                     /gene="scpB"
                     /locus_tag="PC460_001878"
     CDS             27054..27593
                     /gene="scpB"
                     /locus_tag="PC460_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF2600764.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            27755..28384
                     /locus_tag="PC460_001879"
     CDS             27755..28384
                     /locus_tag="PC460_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF2600765.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            28338..28766
                     /gene="ytfJ"
                     /locus_tag="PC460_001880"
     CDS             28338..28766
                     /gene="ytfJ"
                     /locus_tag="PC460_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF2600766.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            29014..29454
                     /locus_tag="PC460_001881"
     CDS             29014..29454
                     /locus_tag="PC460_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2600767.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            29470..30921
                     /locus_tag="PC460_001882"
     CDS             29470..30921
                     /locus_tag="PC460_001882"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF2600768.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKIKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(31012..31680)
                     /locus_tag="PC460_001883"
     CDS             complement(31012..31680)
                     /locus_tag="PC460_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF2600769.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT        12895 a         3263 c         5675 g         9877 t
ORIGIN      
        1 atcatagtta tgaccggata caatgtattt tcaacatttt tcaaatagct tacaactaat
       61 ataaataaag ataaatatcg tataaaagca actaattttt ctctgttcat taaaggcact
      121 cctccaaaaa aataaacttt tactatgaaa actagaataa tatcataaaa gaataaagtc
      181 aataaaagta tttattaata ttgatggaaa taaaatcaat ccaaaaactt gaatagtaaa
      241 attgtatact gacaataatt taaaagtgaa aatttaatgt attggagtga ataatgaata
      301 ataaagaaaa aggtgatttt ggagaaaaag tagcagttaa ttatttgcta tcaaaaggag
      361 caaagatact agaaaaaaat tacagattaa agatagggga aatagatata atagcaaaaa
      421 tggaagatga aattatattt gtggaggtga aatcaaggtc taacataaag tttggctacc
      481 cttgtgaatc tgtaagcttc aaaaaaagaa aaaagataat tggagttgca aattattata
      541 ttataaaaaa taacttaaat aacactcccg taagatttga tgttatagag gtatatttat
      601 tagaaaaaag aataaatcac ataatgaatg ctttttaaaa aagataatgt ttttggaaag
      661 gattaaatat gttaagtatt attaattcaa gcaacttagt aggaatcgac agctttttag
      721 ttaaggttga agtagatgta agcaatggaa tacctagttt taatattgtt ggtcttccag
      781 gcaaagaaat taaagaagca agagaaagag taaagtcagc aattcttaat agtggatata
      841 aatttccaag cacgagaata gttgtaaatt tatcaccagc agatataaaa aaagaaggag
      901 cttttttaga tttatctata agtataggcc ttttaagaga gttaataaaa aaagatgaaa
      961 attacataag agaaagtatg tttataggag aattatcatt agatgggaaa ataagaaaag
     1021 taagaggtat attgcccata attatgggtg caaaaactca gaacataaaa cgtattttta
     1081 taccaattga gaacataaaa gaaagtctat ttgtagatga aattgatata attcctatta
     1141 aaagtttaaa agaatgtgtc gattttttaa acgaagagat aaaagtcgat aaagtaagga
     1201 taatgtcttt tttggatgat aagtcaagaa aagagaacga agaattagaa aaagataata
     1261 gttatataga ttgcaaatat actaagatta ataatgaaga aagtaaatat gatgaagact
     1321 ttaaagatgt aaaaggaaat tattttgtaa agaggagtgc agaaattgct gctgctggaa
     1381 atcataatat gtttatgatt ggacctccag gctcaggaaa aactatgatt gcaaaaagag
     1441 taaggacaat tttaccagat ataagtgtag aagaaatgat agaagtaagt aaggtctata
     1501 gtatattggg tatgataaat gaaactaaag gaataattga taaaagacct tttagagcac
     1561 ctcatcatac aactacaaag caatctttaa ttggaggagg aatggatgca agacctggag
     1621 aaattgcttt agcacataga ggtatcttat ttttagatga gatagccgaa tttgatagaa
     1681 aaatacttga gactttaaga caacctatag aagatggata tgtaaatata tccagagtaa
     1741 agtacagtgc aaaatatcct tgtagagtac tgttagttgc agctatgaat ccatgtccat
     1801 gtggttatta tatgtcagag acagagtgta gatgtagaag taacgaaatt gatagataca
     1861 taaataaaat atcaggtcca ttgttagata ggtttgatat ttttgtagaa gttaattcta
     1921 taaaatattc agattttaat tctttaaaac aagaagaaag ctctcaaaaa ataaagagaa
     1981 gagtagaaaa tgctagaaaa atacagataa atagatttaa gaaggataat ataaaaaata
     2041 atagtgaaat taaagcttac aatttattta aatattgtaa actggaaaaa gaagcttcaa
     2101 aaacggctga gatgatattt aataaataca acctaagcag tagaagttat acaaaacttt
     2161 taaaaatggc aagaacaata gcagatttag aagaaagaga cttgataaat tcacagtgta
     2221 ttatagaagc tttttcattt agaaaggctt attatagtta ttttaaatag ttttttgagg
     2281 tggattttat ggaaaaaaga gatacttatt tatggttaaa atcaattggt ggaataacaa
     2341 caaaaacaat tgaaataata gaaaatgaaa tagtaaatat tgaagatata tttgattttt
     2401 ctgaaaaaga aatatataat ttaaaaaata taagcttgaa tattagaaaa aatatagtaa
     2461 aatatagagg tcacgcttat ttagaaaata taaaagaact actttacaaa aaagcgatta
     2521 aatatatttg taaatatgat aaagaatatc ctgaaaattt aaagaatata tacaacgctc
     2581 caaagttatt attctacaaa ggtgacatag gcttagttaa taataatttt aatatagcta
     2641 tagtaggttc aagaaaacct acagcctatg gaattaattg tgcaaaaaca ataagttgtc
     2701 aactatccca atatggagtt aatatagtaa gtggattagc tataggaata gatgcatact
     2761 cgcacatagg ttgtatgagt ggaaaatcta aaactatagc agtacttggt tcaggagtag
     2821 acaatcctct tccaaaacaa aatttacatt tatcaaataa aatattagaa aatgggggat
     2881 tattattatc agagtataat attaactcga cagtagcccc gtaccatttt tctaatagaa
     2941 atagaattat cagtggttta agtgatggag tagttgttgt agaagcagca ataaaaagtg
     3001 gtgcactaat cacagtggat tttgctcttg aacatggaaa aaatgttttt gcaatacctg
     3061 gtaatataaa ttctcaaatg agtagaggtt gtcataaaat tataaaagaa ggtgcaaaat
     3121 taattgaaaa tatagatgat attttaaatg aatataacat atttaatatt attgataaaa
     3181 aaataaatca aaaatatgat aatataagtc taaatgccaa gtctaagcaa ataattgaag
     3241 ctataaaaag agaaggaaac ttgcatatag atagtatttg tgattatact ggtatagaaa
     3301 taaagtatgt aaattcaata atcaatgaat tagtactaaa tgagttagta gtagaaatga
     3361 ataataaaac gtacagttta aatgtataaa ttaagattag gtagtggggg tgtatggttt
     3421 tatggccaaa acattagtca tcgtagaatc gcctgcaaaa gctaaaacta tagaaaaatt
     3481 tttagggaaa agtcattaca cagttaaagc atcagttggt catgtcagag atttacctaa
     3541 aagtaaatta ggtgtagata tagaaaataa ttttgaacct caatatataa atataagagg
     3601 aaaaggcgat gtaataaaag aattaaaaaa agaagctaaa aaatctaaac aagtatatct
     3661 agcaactgac cctgatagag aaggtgaagc tatatcatgg catttagctc atatactaaa
     3721 tttagatgaa tctgaggatt gtagaataga gtttaatgag ataactaagg atgcaattaa
     3781 aaaagcaatt aaacatccta gaaatattga tataaatttg gttgatgcac aacaggcaag
     3841 aagagtgcta gacagacttt taggttatca aataagtcct atattatggc aaaaggttag
     3901 aaaaggtctt agtgcaggta gagtgcaatc tgttacaaca aagttaattt gtgacagaga
     3961 aaaagaaata aaggcattta ttccaaaaga atactggaca atagatgttg aggctaagac
     4021 agtaaaagac aaagatataa ctttgaaatt ttatggtaaa aatgatgaaa agattgaatt
     4081 agaaaatgaa gaagctgtaa atgaaatact aaaagaaatg gaaggtaaag atttaaaggt
     4141 tgagaagata gaatctaaat caagacgtaa atcagctcca aaaccattta caacaagtat
     4201 gcttcaacaa gaaggagcaa ataagttatt ttttactact aaaaaaacaa tgatgatagc
     4261 acaagaatta tatgaaggta tcgacataga aggtgaaggt actgttggtc ttatatcata
     4321 tataagaact gactctaaaa gaatatctga tgaagcaaaa gaaaaagcta agggctatat
     4381 cgtaaacaat ttaggagaaa actactataa aaacactggt gataaaaaag aaaaaaagga
     4441 tactaaaaag gttcaagatg cccatgaggc aataagacca acctcagttg atagaactcc
     4501 tgagagtata aaagcctcat tgagcaaaga ccagtacaag ttatacaatt taatttggag
     4561 aagatttgta gctagtcaaa tggaagattc agtgtttgat atactaaatg tagaatgtaa
     4621 aataggcgat attgtattta aggcaactgg ttcaaaaatg aaatttgatg gttataccaa
     4681 ggtatataat ttttcagaaa gagaagacaa aattttacca agtatagaaa aaggagatat
     4741 attaaatata gaaaatatat tgccagacca acattttaca caaccaccag caagatatac
     4801 agaagctagt ttagtaaaaa cactagaaga actaggtata ggtagaccaa gtacgtatgc
     4861 tccaacaata gctactatac tgaatagaga gtatgttgaa aagcaaggaa caagtctatg
     4921 tccaacagaa cttggaataa tagttactga tattttggaa aataatttcc ataaatttat
     4981 agatgtagat tttactgctg atatggaaag taaacttgat gttatagaag aaggcaatac
     5041 agattggaaa aagattgtat cagaatcata tgcaccactt aaagaggcta ttgaagaagc
     5101 tcttgaaaat atagaaaaag taaatatgga tgaagaaact gatgagattt gcgagaactg
     5161 tgggtctaat atggttatca aatatggtag atttggaaag tttatggctt gtaaaaacta
     5221 tcccgattgt aaaacaacta gaccaattat gaataaaatt ggtgttaaat gtccaaaatg
     5281 tgaagaagga gatattatac ttagaaaatc taaaagaggt aaagcttttt atgggtgttc
     5341 taattatcca aactgtgact ttgtagcttg gaataagcca acaggagagt tttgtgagaa
     5401 gtgtggttca tatatggttg aaaaagtgac taaatctgaa acaaaaacag tgtgttctaa
     5461 taaagaatgt aataaagaga caaaagagca taatgacgga aaagagtgaa aattgaaaaa
     5521 aatcacataa ttctcttgaa aaaggttcca atctatgata atctttcaag tgtaaacaac
     5581 taatagaaaa ataaattttc attaaatgtg aaaataatat tagaaaatat aaaaggttat
     5641 taaaaggcct aaatttcatg aggagatgat taaatggcaa gtgaagtgtt acaaaaaaca
     5701 aggaaaataa ataaaacatt acaaacaagt ggtggaagca gtgtctcttt tgatttactg
     5761 gccggagcat tgggcgacgt tttaagttct aatgtttatg tagtaagtgc aaaaggtaaa
     5821 gtactaggtc ttcatttaaa tgatgttcaa gacagttcag ttatagaaga tgagtatact
     5881 aagcaaaaga aattttcaga tgaatatact caaaatgtgt taaaaattga tgaaacatta
     5941 gaaaatttaa atggtgagaa gatattagaa atctttcctg aagaacatgg aagattacaa
     6001 aaatatacta cagtagttcc aatattagga agcggtcaaa gattaggaac attggtactt
     6061 tcaagatatt caaattcatt caatgatgat gatttagtaa tagctgaata cagtgcaact
     6121 gttgttggtc ttgaaatatt aagagcaata ggtgaagaat tggaagaaga aatgagaaag
     6181 aaagctgtag ttcaaatggc aataggcact ctgtcctact ccgagcttga agcagttgaa
     6241 catatttttg ctgaattgga tggaaaagaa ggtctacttg tagcaagtaa gatagctgat
     6301 agagttggta taactaggtc tgtaatagta aatgcactta gaaaatttga gagtgcaggt
     6361 gtgatagaat caagatcatt aggtatgaaa ggtactcata taagaatact taatgacaaa
     6421 cttacagatg aattaaaaaa attaaaaaac aatcaataat ctatatttta taggtttaga
     6481 ttagaaaagg tatctatttc aaatagatac cttttttaga cgaattatta aggtaatgat
     6541 aagtatatta aaaaggagtg agaaaatgcc tttagcagat aaaataagac caaaaacaat
     6601 gaatgatgta ataggtcaaa gtcatataat aggtaatgga aaaatattgt ctaagatatt
     6661 acaatctaat tttttaccaa atatgatatt ttttggtcct cctggtgttg gtaaaactac
     6721 agtagcggaa ataatagcag aaagaagcaa taaaagcttc tataaaataa atgctactaa
     6781 ttcatcttta gaagatataa aaaaggtgat atcagagctt ggaagtataa ataatgtaaa
     6841 tggagtccta ttatatatag atgaaataca aagttttaat aaaaaacagc aacaatcaat
     6901 attagaattt atggaaaatg ggagtataac tttgattgct agcactacag aaaatccata
     6961 tcattatgta tataaagctc ttttaagtag gtctactgta tttgaattta aacctttaga
     7021 aaaacttgat gtagaaaaag gactgaaaag agctgttgaa gtattaaatg aagatagcta
     7081 tatggatatt gagtgtaata ataatgctat tgaagatata gcaattcttt ctgatggaga
     7141 tatgagaaga gccttaaata ttttagaagt ggtagtctat tcaactaaac caaataagga
     7201 taatataact tatatagatt cagatgtaat aagggcatct acatttaata aaattataaa
     7261 ctatgacaaa aatggtgata gtcattatga tattttaagt gcatttcaaa aatctataag
     7321 aggaagtgac ccacaggcat ctatacacta tctagcaaga cttataaaag gtggggattt
     7381 gataagtata tgtaggagac ttttagtaat agctagtgaa gatataggtt tagcttatcc
     7441 aaatgctatt gtaattgtaa aggcatgtgt agactcagct atgcaacttg gatttccaga
     7501 agcgaaaata ccattagcag aagctactat tcttttggct acatctccaa agtctaattc
     7561 tgcatgtata gcaataatga aagctatgga tgaactggat aaagagtttg ttaaggatat
     7621 accaaatagt ataaaggatt ctcactattc tggttctaaa gaaataggta ggggttgtgg
     7681 atacaaatat cctcataatt ttaaaaatca ttatgtaaag caacagtatt taccagatag
     7741 cataaaagat agtatttatt acataccaca agaaaataaa actgagaaaa atataaaaaa
     7801 atatttagaa tatctaaata gtgattttta gactgaactt tatgtatcgg gggtagatat
     7861 tatgattata aaacttaact caacttatca cagtagagtt atgaaatact taaaaaaaga
     7921 acctgagtac aatttgttta taatagggga tattgaaaga tatggatatg gaaataattt
     7981 tttaaatata tgggcagatg tgggtgaaca tggtgaaata aaggcaatat tacttaaata
     8041 ttttgaattt atgatatttt attctgatgg agagtatgat gttgaaggtt tttataatct
     8101 acttcgtaat actaattacg aagaaatatc tgggaaaata tgtgctgtag atgctttagc
     8161 aaagagatta ggacttaata atttaaaggt agttgatttt tgcaaacttc aaactaaaaa
     8221 gtttttgata gataataatt gtaatgcaaa ggttaaaaga attaggcttg gaaatctaaa
     8281 aaagacagtg aaactttatg acttaataga tgaatttcac agtacaactt tagagaattt
     8341 aaaaaatgga ctaaggacag gtagaggata ttgtatagag attaacaaac aggtggtgtc
     8401 aatggcaaag agtacttcag aaaataggac tcatgcaatg ataattggag taggaacaca
     8461 tcctaaatat agagctaaag gacttgctac aaaatgccta attaaactat gttcagagtt
     8521 actgagagaa aacaaaattc cttgtttgtt ttatgataat gaagaggctg gaaagatata
     8581 taaaaaacta ggatttgaaa acattggaaa atggggaatt tattcaaaat aaaaaattaa
     8641 aattaatctt gacaaaatta gtagggtata ataaaattat cttgaaagta attcctacta
     8701 aaacagtatg aattagattt ctatatagag gtgataatat atgaagttgt ctactaaagg
     8761 tagatatgga ctaaaagcaa tgtttgaatt agcattaaat caagacaatg gaccagtatc
     8821 attaaaattt attgctaaga agcaaaaaat atcagatcag tacttagaac aaatattttc
     8881 atctttaaaa aaatcgggtc tagtaaaaag tgtgagaggt gctcaaggag gttatttgct
     8941 ttcaaagaat gctgaagaca taacagtagg agatatactt gtagtattag aaggaccagt
     9001 tgcattatct gactgtgtac tagatgaaga tgtatgtgaa aactcaaaca tgtgtgtaac
     9061 aaaaatagtt tgggaaaaga tgaaaaaggg tatagaagat gttatagatt caattactct
     9121 taaagatatg ataaatgact ataacaaaaa taaattagaa aatgatataa caaatattaa
     9181 aaaatagatt tggagatgaa tgtaatggaa aaaagaagat tgtatatgga ttattctgca
     9241 acaacaccta ttaaaaaaga agtattggat gcaatgatgc catacctaac agattatttt
     9301 ggtaatgcat ctagttttca cacattcgga agagaagcaa aagatgcttt ggataaagca
     9361 agagaacaag tagcagccct tataaatgct gaacctagtg aaatatattt tacagcaggt
     9421 ggttcagaaa gtgataactg gacactagag ggtgtagctt atgctaataa aaacaaaggt
     9481 aatcatataa taacttcaaa aatagagcac catgcaatac ttcatacatg tgaatactta
     9541 gcaaaacatc atggatttga aataacttat ttagatgttg atagcgaagg aaaagtagac
     9601 ttaaagcaac tagaagattc aataaaagat actactatac ttataagcat aatgtttgca
     9661 aataatgaaa taggaactat acagcctatt aaagaaatat ccgaaatagc taaaaaacat
     9721 aagatattat tccacactga tgcagtacaa gctacaggta atattccagt agatgttaaa
     9781 gaattaggaa tagatttgat gagtatgtct tcacataaaa tatatggacc aaaaggtgta
     9841 ggagctttat acataagaaa aggtgtaaga cttcataact ttgtccatgg tggagcacaa
     9901 gaaaaaagca aaagagctgg tacagaaaat atacctgcaa tagttggata tggaaaagca
     9961 gcagaattag ctaaggaaaa tatgcaaaat catgtagaaa ctttaactcg tcttagaaat
    10021 aagttaatag atggtgtgtt agaaagaata ccttacacaa gagtaaatgg tagcttagat
    10081 aatagattgc caggaaatgc aaactttgca ttccaattta tagaaggtga aggaatactt
    10141 ttattattag atatgttagg aattgctggt tcaagtggtt ctgcatgtac ttctggttca
    10201 ttagaccctt cacatgtact tcttgcaata ggtttaccac atgaaatagc acatggttca
    10261 ttaagactta cagttggaga ttttacaaca gatgatgata tagattatat actagagaac
    10321 ttaccaaaag taatagaaag acttagaagc atgtcaccac tttatgatga tgcgaagaaa
    10381 caaggtttag taaaatagac ataaagataa agcataaaaa atagacataa attgagtagg
    10441 aggaaaagat aatgcaatat agtgataaag ttatggaaca ttttatgaat ccaagaaata
    10501 tgggagaaat tgataatgca agtggtgtag gtgaagttgg aaaccctaca tgtggagata
    10561 taatgaagat atttttagac atagatggag atgtaataaa ggatgttaag tttaaaacat
    10621 ttggctgtgg ttcagctata gcaagttctt ctatggcaac tgaaatgata aaaggaaaaa
    10681 ctataaaaga tgcattagaa cttacaaata aagctgttgc agaagctttg gatggtttac
    10741 caccagtaaa gatgcattgt tcagttttag ctgaacaagc tgtaaaagca gcccttatag
    10801 attatgctca aaagaacaat atccatatac cagaattgga tggatatgtt atagatgatg
    10861 ctcatgacca tgatgttgaa gaagaagaat aaaaataagt gattgtgtta tgagaagttt
    10921 acaatgatta aaaagaagta ctaatgatat aattaggaca taaaaaatag ctgtttttaa
    10981 aatggctatt ttttattgcc taaattaagt attttaactt gatttagagg ttaattagtt
    11041 aacaaaataa tccataagat tcaaataata aagatgatta tttttttaga aaatagaaca
    11101 agctatattt taaataagta caatacttgc tctatatggt ataataataa gtaatgcaaa
    11161 agatgaggtg aaatttgttt atgaataaaa aagtaatgat aggtatgagt ggaggagttg
    11221 atagctctgt agctgcatat cttttaaaac aacaaggata tgatgtcatt ggtgttacta
    11281 tgaagttgtg gcaagatgat gatgttgttg aaattgaagg tggttgttgc tctttatctg
    11341 ctgttgaaga tgctagaagg gtagcaaata aaataggtat accattttat gttttaaatt
    11401 ttagagaagt gtttaaagaa aaagtaatag attattttat agatgaatat ttggaaggaa
    11461 aaactcctaa tccctgtata gcttgtaata aacatataaa atttgatgat ttttataaga
    11521 aggctagaca aataggttgt gattatgtag ctacaggtca ctatgcaaag atagaaaagg
    11581 atgaaagtac aggaagatat ttgctaaaaa aatctgttac agataaaaaa gaccaaactt
    11641 atgcacttta taatcttaca caagagcaac tagagcatac tttattgcca ataggtgact
    11701 atgaaaaaga tagagtaaga gaaattgcta aggaaatggg tatggcagta cacaataagc
    11761 ctgatagtca agaaatttgt tttgttaaag ataatgacta tgcaaattat gtaaaaaaac
    11821 attctaaaaa gcgtattgaa gaaggttttt ttgtagatac taaaggcaat atacttggaa
    11881 aacataaggg tatattatat tatactatag gccaaagaaa agggcttgga attacttttg
    11941 gaaaaccaat gtttgtaata gatataaatc caataaataa taccatagtt cttggagaca
    12001 atgaagattt atttaaaaag gaacttattg ctaaagatgt taactttata tcaatagata
    12061 cgttagaaga acccttaaga gttcaagcca aaataagata ttcagcaaaa ccatcaccag
    12121 ctactattca tagagtagga gaagatacta ttaaaatagt ttttgatgaa gctcaaagag
    12181 ctattactaa aggtcaatct gtagtaatgt atgatggaga tattgtagta ggtggaggta
    12241 taatcgagaa aagtttataa aaacatctaa aaaattaaaa aaataagcaa taaagtatat
    12301 taagagtaaa taaagtttat aaaataaatt tattgtatat gaaaatatag tattgattaa
    12361 ttttgtaaaa tttagtaata taattagtat attaattaaa aaacaaatac agtgataagg
    12421 aatagtaaaa tatttgatgt cttacagaga gagggaaatg ctggaagccc ttagaatatg
    12481 atatttgaag cagcctttta gtagcaatct tgaacaattt atcttttata aagaagattt
    12541 aagtagagat tgacggtagt tacgttatag ctaaggtttt ccgaagatat catactttgt
    12601 atgataatta gggtggtacc gcgattgtcc tcgcccctat aaatttattt atagggacga
    12661 ggttttttat ttaaacgaaa aaataaaatt tattaggagg aacatgatat ggaaaagatg
    12721 ggattaaatg aaataagaag taagttttta gaattttttg agtcaaaagg tcattatgta
    12781 gcaaatagtt attctttagt accaaacaat gataagagtt tgttacttat aaactcaggt
    12841 atggcacctt tgaaaaacta cttttcaggt gtagaagtac ctccaagtgt gagaatgtgt
    12901 acatctcaaa aatgtataag aacaggagat atagaaaatg taggtataac tgcaagacat
    12961 gctacattct ttgaaatgat gggtaatttc tcatttggag attactttaa aagagagtca
    13021 atcaaatggg gatgggaatt tgttacagaa tggctaaata taccagaaga taagatatgg
    13081 gtgactgtat atgaagaaga tgatgattct tatgatatat gggcaaaaga aatgaatttc
    13141 ccagaagaaa gaatggttag acttggaaaa gatgataatt tctgggaaat aggtacaggt
    13201 ccttgtggtc cttgttcaga aatatatttt gatagaggag aagaatatgg atgtgacaat
    13261 ccagactgta aaccaggctg tgattgtgat aggtatttag aattttggaa ccatgtattt
    13321 actcaatttg atagagatga agaaggaaat tatagtctat tagaaaacaa gaatatagat
    13381 acaggtatgg gtcttgaaag aatgggatgt attatgcaag gcgtagacac tatctttgaa
    13441 gttgatacaa ttaaatctat actagaagca gttgagaagt taacaggagt aaaatatgga
    13501 gagaatccaa aaaatgatat atcaataaga ataataactg accacataag agctgtaaca
    13561 ttcttagtta gtgatggagt gcttccatca aatgaaggaa gaggatatgt tcttagaaga
    13621 ctccttagac gtgctgctcg tcatggtaag ttattaggag taaaagaatt atttttacag
    13681 aaactaatag atgaggttat aaaagtaaat gataaagcat atccagtttt agttgaaaaa
    13741 gaaagttata ttaaaaaagt agttggaata gaagaagaaa aattcaatga aactatagac
    13801 caaggaacag aaatattgaa ctcttatata gaagtattaa aaaatgaagg aaagactgtc
    13861 ctaagtggtc aagaagcttt taaactatat gatacttatg gcttccctat agacttaact
    13921 aaagaaatat tagaagaaga acatctatct gtagatgaag aagcttttaa tgaagaaatg
    13981 gaaaagcaaa aagaaagagc tagaaatgct agaggaaata tggatggaga aagttggaag
    14041 gaagaccctc tatcaaaatt agaatctaca gttgacagta catttaatgg gtatagtgaa
    14101 atttatggtg aaggaactat tgaagctata gttaaagatg atgagctagt tcaaagtgca
    14161 gaagaaggcg ataaggtatc tatagtacta gataacacta ctttctatcc tgaaggtgga
    14221 ggacaagttg gagattgtgg attgataact aatgaaaact tagttttaga agtattaaat
    14281 actaaaaaag gtgctaataa tagtataaaa catataggta taataaaatc aggaagaata
    14341 agtaatgggg acaaagtaaa gacactcgta gatagagaaa ctagaatgtc agctgcaaga
    14401 aatcacagtg ctacacattt acttcataag gcattaagag aagtattagg agaacatgtt
    14461 aatcaagctg gttctttagt aactccagaa agacttagat ttgatattac tcactttgaa
    14521 gcaatatcaa atgaagaatt aaaagtaata gaagaaaaag taaataatgt tattttatca
    14581 tctttagaca taaaatgtga tattatgaat ataaaggaag ctaaagaaaa aggagctaca
    14641 gcactatttg gtgaaaaata tggtgatgag gtaagagtag tttctatggg agattattct
    14701 acagaacttt gtggaggaac tcacttgaca aatacttctc aagtaggaat gttcaaaata
    14761 ttatctgaag gtggagtagc agcaggtgta agaagaatag aagcaataac aggaaaagct
    14821 gtttatgaat acctaaaaga aagagatgga attatctctg aagtttgtgt aaacttaaaa
    14881 tctaaagaag ataatttaat tcaaagaata agttctttat tagaagaaaa taaaaactta
    14941 tctaaagaat tgcatgatat gaaagctaag atgagtttac aatcagttga ttctattttt
    15001 gattctaaag ttgaagtaaa tggagttaat ttgataacta ataaatttga aggtatggat
    15061 atggatacac taagagaaac agcagataat ctaagagata agcttggttc tggtgtagta
    15121 gtacttgcaa atgtagtaga tgataaagtt aactttgtag ttactgccac taaagatgta
    15181 ttagataagg gaatacattc aggtaatatt gttagagaag ttgcaaaaat agctggagga
    15241 aaaggtggag gaagacctaa tatggcacaa gcaggagcta gtgatgtatc taaggttgac
    15301 caagctttaa gctatgcaag tgaggttatt aagacacaag ttaaataaca ttcactcaag
    15361 gaggttttta gtttggaaaa agatttagac tacactatga aatttgaagg aataccagaa
    15421 gataggatga gtgtaggaga tactattgat tttgtatata aagcattagt tgaaaaagga
    15481 tataacccta taaatcaaat tatagggtat ctcctttctg gtgattctag ctatataact
    15541 agtcataaaa atgcaagagc tataattaaa aaatttgaaa gagatgagat acttgaagaa
    15601 gtaatcacgc actatctgaa tagaaagtag gtcaaataaa tgagaaaaaa gattatgtcg
    15661 ttgtttgcaa tagtcattat gatttttgta tcaactgcta gctatggttt tgctgaagaa
    15721 aaaggcaagg tcatatttat tgatatgaat aggaccaatc ttggaagtat gatgaatatt
    15781 ccaatcttga aaaatgagat agaaaagaga ggctatgtag ctctcatgaa tataagaggg
    15841 gatcaaggta ctgatgatag aaggtcttgt gcaagtatgg gtgcaggaac aagagctagt
    15901 gtaactactg aagactatat caattttcaa aaagtagacc aagatactgc taaagctttt
    15961 gaagtcgcaa caggtcaaaa agctaaagaa ataaatgatt taggcattaa tagatctata
    16021 aatgaaaatc ttgagtatgg agaatatgga gcaaatttag ggtcattagg aaaaacttta
    16081 tcagaaaatg gtttaaagac atctgtaatt ggaaatgctg atataattga aaataatcaa
    16141 ttagttaaaa acagaaactt atgtttagta gctatggacc aatatggaag gattgattca
    16201 ggtaatattg ataatattaa taagaaagat tctaaaatgc catttggaat atctacagat
    16261 tatgataaat tactagatga aactaaaaaa tcctataaaa atagtgatgc tatatttgta
    16321 gaacttggag atacatatag attagatatg tatagattaa atctaaatga taatacttat
    16381 gatacaatga agaaaaatat tgtaaaaaat ataaataaat atcttgaaca agtattttct
    16441 atggttggag aaaatgacac tgtttatata gcaagtgcat ttccaagtga cctagattat
    16501 aagaataaga gaagactttc tccaataata aaatttagtg gagaagggaa aggtctactg
    16561 gaatctgcca caacaagaaa ggctggaatt gttgccaatt tagatgttgg agtagatata
    16621 ctaggtacat ttggtcttga aaataaagag atggtaggta gaagttatac tttagtaaat
    16681 aaagaggaca atattagcta tctaaaatca ctttatgaaa ggatagtgtc tatatcttca
    16741 ataaggtcaa ctattataaa tggctttgta ggcgtaatat ctgcttcttg ggtaattgcc
    16801 atgtttgcga ttttctttag aaattatatg ccacataaag agcgtgtatt caaagtactt
    16861 aaggagttta taaaattagg cataataatg ccactatcat ttatgttagc tcctttgatg
    16921 aattttaaaa ctccaatttc aatatctttg ggaatatttg caactacgct aatattatac
    16981 ttgttaggta gaaaattatt taaggacaat gatataaagc acatgggatt ttttgcaaca
    17041 cttactattt tagtcatagt tatagattct gtatttggaa catacttaat gcaaaataac
    17101 ataatgagtt atgatgctgt cataggagct aggtactatg gtgtaggaaa tgaatatgaa
    17161 ggtgtaacta tagcaagtgc agtatttggc ttagcagtat tattacatta taagaaggta
    17221 tctaaattac ttgttgtaat tttttcatta ataatactta taactagtgc atatccttca
    17281 atgggtgcaa atgttggtgg agctatatca gaatgtgtag catatttgtt gtttataatg
    17341 cttatatatg acattaaaat agattttaag aaagctgtat tattagctgt ttcagctgta
    17401 gttgtcgttt ttgcatttgc agctttagat atactttcag gtagtgaatc tcatttaggt
    17461 atgtttgtac agagaatttt cctaaatgga ccaggagaga tattacaaac atttggaaga
    17521 aaaattcaaa tgaatatgca actagcacag acaagtgtat gggtaaatat tttacttgta
    17581 ggaattggag taattgcagt gcttatattt aaaccatcta gacactttag aaagataatg
    17641 aataattttc caatcttgtt taaagggttt attgcatcta tggttggttg tataattact
    17701 cttttagtta atgattctgg aatagttgct gcatctacag catctatata tatacttata
    17761 ccactaataa taataagtat aaatatgata atatttgaaa aaaataacta aaaaaatttt
    17821 tactgttcat ataggattta aataaaaaat gcaatatgat tattaatgta aactggatgc
    17881 ttacataaaa aatcatattg caatttttat gattgataat gtaaaattct atgaagcagt
    17941 aagcctgtga ccttagttat gagaggttca gaatatgtat ttaatctata gaatataaat
    18001 agataaagga ggaactatgt tagatggaag aataatgggt cttgatgttg gtgataaaac
    18061 tattggagtt gctgtgagtg atttgatggg attaactgca caaggtgtaa aaacaataaa
    18121 aagagttggc aagaaaaaag atattgaaga gctaaaagca ataataaaag aaaaacaagt
    18181 aaataaaatt gtatcaggat taccaaaaaa tatgaatgga actttaggtc cacaaggaga
    18241 aaaagtaatc aagttttgtg aattggtaaa agcagaaaca ggtattgatg tagagttttg
    18301 ggatgagagg ctttctacag tagcggcaga gaggtcttta ttagaagcag atgttagtag
    18361 acaaaaaaga aaaaaggtta tagatatgtt ggcagcagta ataattttgc aaggatattt
    18421 agattttaaa ataaattcat aaaagaacta taaaattgaa tacttatgat atataatata
    18481 aataataaaa ataaaaataa atttgaggtg aatgacatta tggaagaaaa tataataaat
    18541 ttaatagacg aaaatggcgt tgaaagccaa tttgaaataa tattaacatt agaagcagaa
    18601 ggaaaagagt atgcaatatt aatgccttta gatgatgaag aagctgaaga agctttaatc
    18661 tttagaattg atgaggatga agaaggtgaa atcttaatac ctttggaaag tgatgaagag
    18721 tatgaaactg ttgtagctgt gtatactgct ataatggaag aagaaggttt aaattacgac
    18781 gaagatgaat caaatggact taactagata agtaattaag tttgtatata aaaatcagat
    18841 atttattata tttgctttaa ttaaacctta ttaattgtaa aagcatttat agtatatatc
    18901 tgtttttttg ttttttatta aaaataccaa aaaatttatg aattttggtg aataatatat
    18961 cttttatggg tatatattta tattatgaga ataaattaaa tttatttaaa tgtcttgaca
    19021 aaaaatgaat tatcaattac aatattattt aataaagatt atcaattgaa aatagtaagg
    19081 gtgatgttat ggcaaataca atggatttat taaaagacaa gttaaaagaa actggtttta
    19141 agattacgcc acaaagaaga gcaatagttg aaatattgct taaacatgac cattcacatc
    19201 taagtagtga ggaaatctat gatttagtta gggttgactg tcctgaaata ggcttagcta
    19261 ctgtatatag aacaatgcag ctattggatg aaataggatt aatatcaaaa ctaaacttag
    19321 acgatggatg tataagatat gagattagtc tacataaaga agactgtcat aatcatcatc
    19381 atcttatttg taaaaattgt ggaaagataa tggaagctaa agaagacctt ttagacaata
    19441 tagaaaaaga gatacaatca ttgtacaaat ttaaaatact tgatcatgat gttaaatttt
    19501 atggactatg tgacgagtgt aatggagtaa gcgattcaga agaataaact tctattgctt
    19561 aatgtcgatg gaagaatagt atagaacttt tatattctat gctattcttc cattttttat
    19621 tataaaccat ctataaaatg caaatataca gttttcaaag tgattatagt tttaatattg
    19681 aaaatataat taatcctata agtacaaaaa tgaatgattt ttctatgaaa ttactaaatg
    19741 gtcctccaac agttaaaaac cctaaagaaa ttttcttttc gcttagggga taaaaaattg
    19801 gtattccttg ctttgtaaat aaatctccta aaaacatatg gcttgcataa ccaatcgtac
    19861 cataaaaaga taaattatta atattagtga ttaattctat ctgttttagt aaaaaatata
    19921 ctattatagt tgcaaatatg ctatgagaaa aacttctgtg ctttaaccag gggaaaattg
    19981 acaacattag acaaaaaatt gtaaaatata tttcaacttc tatgatgtat aagcatatag
    20041 ctaatagtat aaatattaat gatagaaaaa cttttcttag aaatgtatgg gttagttttg
    20101 cttcaataat tataattgca acaaatgtaa ttattgcgct taaataaaaa ttatcattta
    20161 tatttattga aataaagaaa ataataatat taacagcata tataaaaatt ctatatatgt
    20221 atttggatac attgtttttt agaatgaaat ttgagaccat agaattagat ttatccaaat
    20281 cgggtagtac agcaaaagta gctgaaacta aaatatcaac taaagatata ggaattttaa
    20341 atattaaaga tgtctggata gctgttaaga ttcctattgc acaatgagtt ttaccacgca
    20401 taaatcctcc taaattatcc gatattaaat catataatat ttagaggata tcaaaataaa
    20461 ataggtttgt caaaataata cgttaaatgt attttaaata tagcttaaaa tatgatacga
    20521 attatggtac aataagaata actggtagcg agtgcgtgat ttgatattat ttatgattgt
    20581 agaacgtgtt aaaaaattaa atagacagaa ggagatgatg caattgttca aaaagaacac
    20641 caataagata aaggtaatgg ccttgggtgg acttaacgaa gttggaaaaa atatgactgt
    20701 tgttgagtat aaagatgaaa taattgttat agatgctgga cttagttttc cagaggatga
    20761 aatgttagga gtagatatag ttataccaga tataacttat ttagtaaaaa atagggataa
    20821 aataaagggt atatttatta cacatggaca tgaagaccat ataggagctc ttccatatat
    20881 attaaagaaa ataaatgtac ctgtatatgg ggcaagactt agtataggtc ttatacaagt
    20941 aaaacttaaa gaacataaga tgaataatgt taagttaaat gttataggtc ctagacaagt
    21001 tataaagctt gataatatgg aagtagagtt tttgaaaaat aaccatagta taccagatgc
    21061 ttactctatt gcaatacaca cagaccaagg tataatatac cacacaggag attttaagat
    21121 tgacttgact cctattgatg gagatgttat ggatatgcat agaatctgtg agttgagcaa
    21181 aaaaggagtt cttttaatgc tggctgatag tactaatgca gaaaaaccag gatttactat
    21241 gtctgaaaag actgttgggg taggtcttga cgaattattt gcaaaaggaa atggcagaag
    21301 aattatagtt gctacctttg catccaacat acacagatta caacaaataa taaatactgc
    21361 tgagaagttt aatagaaaag ttgctatatc aggtcgctca atggtcaatg tagttggagt
    21421 tgcaaaagaa ttaggatatc tagatatttc tgatgacatg cttatagatt taaatgatat
    21481 atgtaagtat gaagatagtg aattagtgat aataacaact ggttcacaag gtgaacctat
    21541 gtcagctctt gctagaatgg ctttttcaga gcacaaaaaa gtagaaataa agagtggcga
    21601 tttagttata atatctgctc atccaatacc aggaaatgaa aaattaatat caagagtaat
    21661 caatttctta tttgaaaaag gagcagaagt agtatacagt gatatagctg atatacacgt
    21721 ttctggacat gcctgtcaag aagaattaaa acttatacat gcattagtta gaccaaagtt
    21781 ttttatgcca gctcatggtg aatatagaat gttaaaaaga catgctgaaa tagctgaaca
    21841 gcttggtatg gataaagaaa atatttttgt catgcaaaca ggagatgttt tagaattaga
    21901 taaaaactct gctaaggtag caaatcgtat acaaacagga aacatattag ttgatggtct
    21961 aggagtaggc gatgtaggta atatagtttt aagagataga aagcatttat cagaagatgg
    22021 tcttatgata gttgtagtta ctatttctaa ggatgagggt aaagttttag caggtccaga
    22081 tataatttcc agaggattcg tatatgtaag agaatcagaa gatttaatgg atggagcaaa
    22141 ggatataatt aagaatgttc taaatgaatg tgaagagaaa aatattaaag aatgggctta
    22201 tttaaagaat aatattaaag aaaacctaaa agaatattta tatcaaaaaa ctaaacgaaa
    22261 tccaatgata cttcctatca taatggaagt ataatataaa aaaactataa atttgaaaat
    22321 tagacctctt gtaattttta aagtaatatc aaaaatccaa ttgatattat cttaagaatt
    22381 acaagaggtc tttttaatac ttgatttaaa aaaatatata tattacataa aataccaatt
    22441 ataacaatct tttattagta aaaactactt atatataaat tttactgata ggaggattct
    22501 tatggatgat gtttcaagac aaaatgctat aaaagcttta aaacaaacaa aaatggaaat
    22561 agcaggagag tatggaatga attatgaaga tgcttttgaa ataatagaaa atgcgtctaa
    22621 taaaggtgtt ttagaaggtt actttaaaaa gcttgaaaag aaaaaaaatt taggtcaagg
    22681 tatatctagg catttagaat aaaaaaagtt aaatgtgaga aaataagttt aaataaactt
    22741 taagaggtga catcgatgaa aaaattagct agtactgctt tggctatatt aattgcgctt
    22801 actcccttaa gcttttcttt tgcaaacaat aaggaaaatg ctgatgccaa ccaactaaat
    22861 atatcttcaa aatcagcaat tttgatggat gttggtagtg gacaaatttt atatgaaaag
    22921 gatgctcata aaaagcttcc tcctgctagt gtaactaagg taatgactat gctacttata
    22981 gtagaagctt tagattcagg caagattaaa cttgatgatg aagttcaagt aagtgaaact
    23041 gcatctagta tgggtggtag tcaaatattc ctagagccag gagaaactca aaaggttgat
    23101 acattgttaa aaggtatagc tgttgcttct gcaaatgatg cttgtgttgc tatggctgaa
    23161 cacttagctg gaagtgttga agggtttgtt gatagaatga atgctaaagc aaaagagtta
    23221 aatatgaatg atactcattt tgcaaataca aatggacttc cagttgcaaa ccactataca
    23281 tctgctcatg atatagcttt aatgtctaga gaactattaa aacacgaaat gataagtaaa
    23341 tacttaacta catggatgga taaggttgta gtagggaaga aacaagttac agtaggtctt
    23401 gcaaatacta acaaacttat aaagcattat caaggagcaa ctggtgttaa aactggcttt
    23461 actcaagaag cgaaatactg tttatcagca tctgcaaaga gaggtaatac tcatctagtt
    23521 gctgcaactt taggagcaga gacttctcca gaaagattta atgatgcctc tagcctttta
    23581 acctatggat ttgctaatta tgaaagtgtt aagttatgta gtaaaggtga taatatagca
    23641 acacttacat tggataaggc tgatgaaaac aaagttaaat tggttgctaa agaagattta
    23701 aatgctctta ttaaaaaagg aagttctaaa gaatttgaga agaaaattga aatagtaaaa
    23761 aatcctaaga tgccaattaa aaaaggaact gttttaggaa agataaagat atgtaaagat
    23821 aaaaaagtaa taggcgaagt ggaattaata aatactaaag atataaataa ggcaagctat
    23881 ctacaaatgc ttcaaagaat aattgataat atgatataaa taattatcag atgaaatata
    23941 attataatac acaaagatat gattataata cacataaaaa atacccttat gtaattattt
    24001 gccatagggg tatttttatt tactatatag aagtatttta taatcttatg atttttatag
    24061 aaatatagaa aagtttatgt atcttaaaat atcatttgat ttaatatcat atatagggtt
    24121 gtttaacaat tatctaagta aaggcatact accagtaagt ttattcactt tctttttatt
    24181 cccataaatt gctatcccaa aatactgtaa ctcatcttct ggaacactag caatcttttg
    24241 tgtaaattct tcataaagat tgcaactttg tgctacatct gaaaaatcta caacagttaa
    24301 atcgttaaat tccaaagtat ataatttttt acgtaaatct tttattattt ctttgtttcc
    24361 ttttaaaata ggtacaggaa tgtcaataat tcctaagtgg ctattacctg ttttgtcaat
    24421 tacatctggc ccaacagttt ctggggcatg ttttcctagt gtaattccca ttatagctgc
    24481 tgtattggat ataattccca ttggtaaatt ttcgtctatt accatgacac attttgaatt
    24541 tttattttcc atttttattt ctccttttta ccttatttaa agttgtttga atcatcattt
    24601 ataaatatat tttgatactg ctttggtgta agaccaataa aattcttaaa aaagtttgta
    24661 aagtgacttt ggtctacaaa tcctgtttgt atcgcagcat ctataggttc tacgcctttt
    24721 tctaaaagct ttttagcttt gtttacccgt atattttcta ggtatctata tggtgtaata
    24781 ccttttaatt tcgtaaaatt acgaagtaga gagtacttat tcatacctgc tatagtgctt
    24841 aattcgtcaa gtattatatg ttcagcataa ttattttcta gataatcaca tactgcttga
    24901 atttctatat ttgtattttc caagttagat tgcaaatttg gctctgtatg ttcttcaatt
    24961 aattgttcaa ttaggaagaa aaaaagctct tcctttttaa aatccaattc ttcttccatg
    25021 attatatagt gtaattcttg tagtagaggt actaactcac tgcgaaatat aacaggttga
    25081 ttaaattttg gtaaataatt tttacctgta atttcaaaga cggttttttt cattatttct
    25141 ggcttaatat taatacatct gtaatcaagt actttatcat ctatttgttc acaagtatga
    25201 ctatcaaaag ggttaaaaag tagcaaatct ccagtacttg tggtatactc tttattttta
    25261 caagataaat atctttgacc tttttctata aaaccaatta cataatgttc atgaaaatgg
    25321 tttgggaatt tttgcattat gcctttaaat ctatatgctt caatttttaa ttccctatca
    25381 aagcatactg tgcgttcttc ttgtctcact taggtaacct cctttcatgt tatacaaagt
    25441 gtaacacata tttttttata cttcttgtat aatattgact aagagacaat aatgtcaatt
    25501 catgtcaata taatattaca cttacagtag gtgtaattat tatattttca caatgaatat
    25561 gatataataa taaaagttat aaaataaaaa gcaaatttta attttatgcc tagaagttaa
    25621 aattattata atgtaaaata attttgatta taagctattt aattgttata ataatattaa
    25681 aaatttaatt taggagatgt acattaatgt taagtatgaa tataggagaa atagtagcaa
    25741 gtttagttgg tatagctatg gcaatatcaa tacatgagtt tggccatgct tattcagctc
    25801 acttattggg agatgataca gctaaagctt atggtagaat gacattaaat cctgtaagac
    25861 atgttgaccc aataggtctt ttggctatgt ttatccttaa gatagggtgg gcaaagccag
    25921 taccagtaaa tccaaataac tttaaaaact ataagatagg aaacctgata gtttcacttg
    25981 caggcgtttt ttgtaatgtt ttgactgcta tactgtgtgt aattatcaat aagtatgtgc
    26041 atatgtatgc tataaataca atagctgaat atgtgttttt atatagtatt ggatttgcag
    26101 cttttaactt acttccgata cctccacttg atggatgggg agttatatct acatttgtac
    26161 catataaatg gaatgaatat ttgtataaat ttgaaagtat gagttatata attttattaa
    26221 ttgctttatt tactggtatg tatagaataa tattaacacc aatatatgca gtgttgctaa
    26281 aaatagtata tttatttgtt taatataata aacagattaa agagggattg atatgaagta
    26341 taatatacaa ttacaagtct atgagggacc attagattta ctttatgatt taataacaaa
    26401 gcataaaata gatataaagg atatatccat aatagatata actaagcagt atctaaatta
    26461 tttgaaaatg ctagataaaa tggatttaga gataactagt gaatttataa ctatggcatc
    26521 aaaactttta gagataaagt ctaaatatct gctttataag caaaaagatg aagaagaaga
    26581 cccaagaata gaacttatgg aaaagttaga agaatataga aagtttaagg tagcatcaca
    26641 agatataaaa gaaaatataa cttatgttaa tgagagattt tatagaaata aagaagagat
    26701 aattattgat gacaatgtgg atttagaaga tatatcaatt gaagcaataa aaaatatact
    26761 tccatacata tttaaagtta aaacatcaca gatagaaaat gctaatgatg agaaactaga
    26821 taaaatagta aggaagaaaa taatatctgt agaagaaaaa atattatata taagagatat
    26881 tataaaagag aagattgaag ttacttttac aaatattata aaatcatatg aaaatgatga
    26941 aataatagct acttttttat ctatattaga gcttataaaa gaaaaagaaa tagtcgtagt
    27001 acaagatata ttttttgatg atattctaat aagaaaaagt tcggagtgtt aaaatgaaaa
    27061 gagaagatat aaagtatatt atagaatctg ttatgtttgc atatggtgag ccaataagta
    27121 taaaagaatt aaactatata ataaataaag aactttcatc aaaagaaata gagattatgt
    27181 taaatttact tattgaggag tacagagaac aaaatagagg tattcaaata atcaagttag
    27241 agaataagta ccaaatgtgt acaaataaag actatgcaga atatataaag aagattattg
    27301 aaccaaagaa aaagaaaagt ttgagccaag ctacattaga aactcttact ataattgcct
    27361 acaaacaacc aataacaaag gtagaaatag aggatataag aggagtaaaa tgtgataagg
    27421 tattacaaac tttgtttgaa aatgaactta taagagaagc tggtagattg aataagatag
    27481 ggaaaccaat aatttataaa acaacagatg aatttttaaa gttgttgaat atagaaagtc
    27541 ttgaagaatt gccacctata gaaaattatc aagaagtggc aacaaatgaa taaaaatata
    27601 aaatcacaat aatttagtat gtaattttaa attttaaaag atttaaaatg ctctaagttg
    27661 ttgtgttttt ttgtataaat ataaaattat aattctctta aaataaatat ggataattaa
    27721 gtataaatag taattttttg atgataatat aaacatggag cttaatgaat tatttaattt
    27781 taagtattta ataatagctg taatttttat tataagtgta actatgttga ttttaatatc
    27841 tcatataaac atcttaataa tagctgatat aaaaaacaaa gatatttgtt taaagcttaa
    27901 tataaaatac atgtttaatt taataaatat aaatagacaa ctttatccag ctgaaaatag
    27961 taaaaataat gataaaaaag aggggatgaa gaataatata gattcaagta tattattagc
    28021 tgatgacctt ttgagcattt atagattatt aaaaaagata aaaatacatg aactttattc
    28081 aaatattaat tttggaactg gaaatattgg cttaacatct tctgtatatg tattgataaa
    28141 tactttgtat ggaaatttat ttaatatgat tgatgcagaa aaaatgtatt taaatgtaaa
    28201 tccagatttt acaaaagatt atgttttagg aaatataagg atacatataa ggccaagaat
    28261 aaaagcttta tttaatatta taataatgat taataaaatt atgaataaaa ataaaggaaa
    28321 taaggaaggt gatagtaatg agagcaacag gttcgataca gagtcttatg gaaacaactc
    28381 ttgaaacaat taaaggttct atagatgcaa atactattat aggagaccct ataaaaacag
    28441 atactacagt ggttgtacct atatcaaagg ttacaattgg ctttggtata ggaggaggag
    28501 aatactcaaa aggatatgat gataaagata gggaaattga attaaaaaat gaaaaatcag
    28561 atacgaattt tgctggaggt agcgcagggg ctatatctgt acaaccagta gcatttgtag
    28621 tagttgaaag tggagagact agaataatga gtttagacag taatatcaat ctagtggata
    28681 atatattatc tataactcct agagtattgg agaaaattca aaatatatca caaagtaata
    28741 agaatcaaga taaaaacaat atgtaagaat aaaatatttt taaatatgta tatagtttta
    28801 ggtaaagtag tggggttagt ataaatccca ctattttgtt gtgtacttta aacatttata
    28861 tctaataaga ttttagttta aaacaattta aatagataat aaagttgata tatagtataa
    28921 ttataaaaag aaaagaagta ttatattata gatatataaa cgcatttgag atataagagg
    28981 gagttataaa tcattagttt tgaggtgaat gttatgagcg ataaagaatt atctattttg
    29041 ttgttagaaa gtatgaataa ttttcataat ttaataaaga ttattaataa cgaaagatat
    29101 aaaaaagata aggttcttac agaaagacag ttctttgcct tagtaaaaat aagaaaacat
    29161 gataaaatag agcttaaaaa tctaagtaga gatttgcatg tatctacttc tagtctgtgt
    29221 atactactta ataaattggt tgagcaagag tatgtatata gggaagaaga tagtagagat
    29281 agacgaaata ctttttatgg aataactaaa aatggagaaa agatattgga taatgaaata
    29341 ctaaagtttg tatctataat aagtgataaa atggattgcc tagatataga taataaagat
    29401 aaattattta cttctttgga agagtctaaa aatataatag aacaattatt ttaaaaagga
    29461 gaatttatta tgaatataaa gtattctaaa gaaatattaa aaagtttaga aaatgctata
    29521 acagtagtag ctctatttga gggagaagat gtttctacga catttgataa aataaatatg
    29581 ataagcagaa attttaattc taaagaaaat agtatagata ctgtaacatt gattgaaaat
    29641 aacaatttgg taaatgtttg tcttgttggt tttggtaaga gagagttatt gaatagagaa
    29701 aaaattagaa tcataggtgg taatttgtgt aaaaaattaa aaaatatatt gaaaaatgaa
    29761 gcatataaga gtagtgatgt taatgtactg aatcttaatt taaatagtga tgaaataggt
    29821 tcttttgtag aagggatatt acttggaaat tataagtttg ataagtataa gacaaaaatt
    29881 aaggattttg aaagaaaaga agttgatact ataactatat ttactgaaaa agaagttgag
    29941 tgtcaaatag aaaaggctaa aatattagct aattcaacta taatagctag aaatctagtt
    30001 aatgaaccat caaacgtaat atatccaaaa acattagcag tagaaacagt taaacttggt
    30061 gcagagtttg ggtttaatgt tgatgtttat gaagaagaaa aaataaaggc attgggaatg
    30121 gatgcttttt tcgctgtttc aagaggctca attaataaac caagattcat agtaatgaga
    30181 tactttggag ataaagaaag tacagatata ttaggtttag taggtaaagg attgacttat
    30241 gatactggtg gatattcttt aaaatcaaat gcttctatgc tagatatgaa gacagatatg
    30301 gctggtgctg cgagtgttat aggtgctatg tgtgctatat ctcaaagtaa attaaagaaa
    30361 aatgttatag cagtagtggc tgcatgtgag aatgctttat caggaggttc atacaaacct
    30421 ggagatataa ttagttctat ggcaaagaaa actattgaag ttttaaatac ggatgcagaa
    30481 ggtagactta cattagctga tgcaatatac tacattataa acaatgaaaa agtaacaaag
    30541 gtggttgatg tagctacatt aactggtgct gctttaacat tgttaggtaa tgttgcaact
    30601 cctatagtaa caaataatga tgatttttat tgtgaattag aaaaagctgc cactttatca
    30661 ggggaaaggg tatggaagat gccaatttat gatgaattta aagatatgat aaaaggagaa
    30721 gaagcagacc ttaaaaacac tggtggtaaa aatgctggat gtataacagc aggagctttt
    30781 attggtgaat ttgttgggaa tactccttgg atacatatgg atatagcggg gacaagtaca
    30841 tcatcaaaat caattggata caaagcaaag ggggctacag gagaacctgt tagaacattg
    30901 tactatttgg cagaaatcta aaaaacaatt ataaaatata atttataaag ccacgtggat
    30961 atttttgtaa tttaagatta tctatgtggc tttatttttt atttaaataa attatttact
    31021 ctttttcttt ttttcaagta taaaagtatc tctattaaat aaaagagcag gtatgtacat
    31081 aatactagta acaaaaataa acgtaaaaat agcagttgtg ctatgactta aaaaaccata
    31141 atagtaccta gagttggtga atgtgtttag taaaaatatg aatgaaaaat aaataaaaca
    31201 tatagctaag ttatctctaa gtgcatatct gttaactcta aattttctaa catcaaccat
    31261 atatatatct gcataaatta tcaagcactt agagaagata aagataaaat atattaagct
    31321 aggaaattca tagcccaagt ttttagaaaa aatgagttct ccaactgaac aaactaaact
    31381 ccaagaaaaa aatatattaa aaagatgata ctgcttaaaa attagtatgg ctatacacag
    31441 atatatacaa atataattga ttccaattgg taaagagttt agagtactat aattacctga
    31501 agaaataagt aataattgct ctagagccat aattattaca agtaatgtac aaattatctt
    31561 ctcaactgta tagctataag gtaaaatatt tttggttagt tttggacaat agtataaaaa
    31621 taatccaaat ataaagagac ttacaaattg ttcactagaa aaaagaaaaa ttttaaacat
    31681 ataacattct ctcctataat aaatttaata
//
LOCUS       DAMJWU010000037        31666 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMJWU010000037 DAMJWU010000000
VERSION     DAMJWU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.037"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            177..833
                     /locus_tag="PC460_001884"
     CDS             177..833
                     /locus_tag="PC460_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600770.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1341..2612)
                     /gene="sleC"
                     /locus_tag="PC460_001885"
     CDS             complement(1341..2612)
                     /gene="sleC"
                     /locus_tag="PC460_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF2600771.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2857..3573
                     /locus_tag="PC460_001886"
     CDS             2857..3573
                     /locus_tag="PC460_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF2600772.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3652..4962
                     /locus_tag="PC460_001887"
     CDS             3652..4962
                     /locus_tag="PC460_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF2600773.1"
                     /translation="MTELPNEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5062..5598
                     /gene="lepB"
                     /locus_tag="PC460_001888"
     CDS             5062..5598
                     /gene="lepB"
                     /locus_tag="PC460_001888"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2600774.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5764..6300
                     /gene="lepB"
                     /locus_tag="PC460_001889"
     CDS             5764..6300
                     /gene="lepB"
                     /locus_tag="PC460_001889"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2600775.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6310..7065
                     /locus_tag="PC460_001890"
     CDS             6310..7065
                     /locus_tag="PC460_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF2600776.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            7139..8806
                     /locus_tag="PC460_001891"
     CDS             7139..8806
                     /locus_tag="PC460_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF2600777.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            9379..10308
                     /gene="glsA"
                     /locus_tag="PC460_001892"
     CDS             9379..10308
                     /gene="glsA"
                     /locus_tag="PC460_001892"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF2600778.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            10406..10969
                     /locus_tag="PC460_001893"
     CDS             10406..10969
                     /locus_tag="PC460_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600779.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            11457..12293
                     /locus_tag="PC460_001894"
     CDS             11457..12293
                     /locus_tag="PC460_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF2600780.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKKELKMIKEILS
                     SK"
     gene            12577..13572
                     /locus_tag="PC460_001895"
     CDS             12577..13572
                     /locus_tag="PC460_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2600781.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(13829..14233)
                     /locus_tag="PC460_001896"
     CDS             complement(13829..14233)
                     /locus_tag="PC460_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2600782.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            15074..15988
                     /gene="queG"
                     /locus_tag="PC460_001897"
     CDS             15074..15988
                     /gene="queG"
                     /locus_tag="PC460_001897"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF2600783.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKNRQQKGYLTG
                     MEEPIIESRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            16094..18331
                     /locus_tag="PC460_001898"
     CDS             16094..18331
                     /locus_tag="PC460_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2600784.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTKQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            18529..19746
                     /gene="nth"
                     /locus_tag="PC460_001899"
     CDS             18529..19746
                     /gene="nth"
                     /locus_tag="PC460_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF2600785.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKKEINKKGIDKKDIIEKKEINKKEMDKKDIIEKEEINK
                     KGMNKKDIIEKEEINEKEIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKG
                     ETEKKTKKSSSNKATQKTSKETKSKFKIKNESANEKDVNKILDELEKLYPDAKCELNY
                     GTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKS
                     KSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVF
                     RVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIK
                     EDCNYYKELNETK"
     gene            19940..20410
                     /gene="trmL"
                     /locus_tag="PC460_001900"
     CDS             19940..20410
                     /gene="trmL"
                     /locus_tag="PC460_001900"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF2600786.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            20446..21294
                     /locus_tag="PC460_001901"
     CDS             20446..21294
                     /locus_tag="PC460_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2600787.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKKLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            21599..22603
                     /locus_tag="PC460_001902"
     CDS             21599..22603
                     /locus_tag="PC460_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2600788.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            22729..23841
                     /locus_tag="PC460_001903"
     CDS             22729..23841
                     /locus_tag="PC460_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2600789.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATEYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            24331..25092
                     /locus_tag="PC460_001904"
     CDS             24331..25092
                     /locus_tag="PC460_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600790.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            25095..25913
                     /locus_tag="PC460_001905"
     CDS             25095..25913
                     /locus_tag="PC460_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF2600791.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            26093..26695
                     /locus_tag="PC460_001906"
     CDS             26093..26695
                     /locus_tag="PC460_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF2600792.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    27154..27391
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            27455..29374
                     /gene="thrS"
                     /locus_tag="PC460_001907"
     CDS             27455..29374
                     /gene="thrS"
                     /locus_tag="PC460_001907"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF2600793.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            30142..31233
                     /locus_tag="PC460_001908"
     CDS             30142..31233
                     /locus_tag="PC460_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2600794.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT        12647 a         3016 c         5825 g        10178 t
ORIGIN      
        1 gataatggtg gatagagtag taagaaaata aatagataaa gggatagaat aaatttaaat
       61 atggaaaatg aatatctaat aataagtgat taataaataa ttgtagatag attgtaaatt
      121 aaataaatct ttaatagaaa ggagaactat ttatatatta aatagtattt ataaatatga
      181 aaaaaggcga ttttatatgg agtggagttc ttatttgtat tattgctgtc ttagcaatac
      241 caagtagtag agaagtattt atggaagcaa caggtgcaca tccatatatt ggaggttttt
      301 taaagtttgc tgtattagct acaatgggag atttacttgg tgctagaatt ttgtataagg
      361 attggaaaat accagtagga gtattttata gagcaatagt ttggggaata ataggtgtag
      421 ttaccacata tggaatgtca atctactcat caggtgtagg tcaagcacaa gtggatggta
      481 tgcttccatt tgagggaata gcttttgcaa atgctctatt taagagtgca actatgaata
      541 tactaatgtc accaactgta tttttattcc ataaggttat ggactgtttt atagataaaa
      601 aatatgaggt tggaaagggg attaaggtaa aacttaaaga tgtggtaaat attgtggact
      661 ggaatgctta ccttggtttt agtgttttaa aaactatacc atttttctgg ataccatgtc
      721 atacaattgt attcttattc cctgctgagt atagagtaat tgcttctgca tttgcatcta
      781 tagctttagg tctaatattg gctttagcta ataagtctaa agttgcagtt taggttcact
      841 gatgaagata gtagtattat ttaaattagg aataactttt taaattaata ttatttgtat
      901 ttataataaa taataattta tataagaagt aatattctaa gtgatggata gtgttttaaa
      961 taataagaag taatactcta agtgatagat agtgttttaa ataataagga gtaatactct
     1021 aagtgagaag tagcatttta aataataagt gagattttaa ataaaaagta ggtttttcgt
     1081 tagaatagca ttttattaaa cttaaaaata aatataatat tttaaagaaa tctaagttgc
     1141 taagcactgt ttttaagaag cttaaaatcc cccatttaaa gttttaagat tctaaaaaaa
     1201 caagttataa ctaaaaaatg gctgttaaga ctacaatact tataagtaat acttatggta
     1261 tatattagtt cataacagcc atttttattt taaattaata attttattca ataatttgag
     1321 tttatgttct aaatcaagct ttaaattaaa ggatttaaag aagctattct agttgtagct
     1381 acataaactc tggatatttc ataccatgtt ttaaaatcta ctactccgct ttgaggtaaa
     1441 ccaaagattc cttggaatat cttaacagcg ttttctgtat ctgttccata gataccatct
     1501 tctataactt ttggtacagc tggataagaa ttagatatag catttaactg attttgtatg
     1561 gttctaacat actgtccaga agaaccaact tgtagtgtag taccagggaa tgacactggt
     1621 acaccactta ctataggcgc tctttcaaat actatctcat ctccatagaa atatcttagt
     1681 atactctcat agtcatatcc ttggtcacct aagtctttac tgccccattg agtcatttgg
     1741 tcaggacatt gggatttttg tccatcacaa tattgtgcta atagtggctg tcttgatgta
     1801 ggaggtcttt ttataaaagt attaaaaata gcatctacaa ctacgttaat tggttcaaat
     1861 aagtttctat tatttataaa cttatggtcg tatgctgttg ttgaagttat tgtaaagtta
     1921 tatcctttat ttctatacca ttcagtaaag actctattta aagtaaatga tattatagct
     1981 attacatttg cataaattgt ctgttcaggc caagttgaat atatttcaga tgatgctatg
     2041 tttttaatgt actctttaaa tggaatccaa tagtttggag ctgatgaatc ttctggcaaa
     2101 ccatcatgaa ctactataaa ttcaggcact acagggttat ctaatacaac gaaaccagta
     2161 ggaggtggta atggttttaa gttactttct ggaatcttag gaggataagt accatataga
     2221 gtatgttctc ctatgtcaaa aatcaactca ctttgtcttg agaaagaacg cttacttctt
     2281 gttctaggag acatgggaac tccttgtctt gcttcaattg tagctaacaa ctgtgttcct
     2341 tctatgacaa ctgtctcata tccatctgct atggcttcca ctatatactg gctataaggt
     2401 ctaacatcag atggttgtag agagtaatct acatctggtg ctggtaatac taaaccagtc
     2461 acttgaccag attcattact tcttaggttt tggtataaag ttgatgagct ttggctacca
     2521 ttagacattg aatatatgtt tactactgca ttttgaatag gtctattgtt agtagcatca
     2581 attatgctta ctgttaagaa accatcttgc atcaaatcac cctttcttta aatgaattta
     2641 gttcccttat atttcaagat atgaatatag cttaaaattt gttaaataat atagatataa
     2701 atttaaattt ttttgagtat ttgagtatat ttgtatttaa ataatttaaa ataatttaat
     2761 gtatatttta gtatgtattt aaaacacgaa taagttaaaa ataagtagat gatataaatc
     2821 ataaataaat caaaaatttt gaaaggaaaa taatctatga agctagattt atttttatta
     2881 gcaatagttc ctatattgat aggaatgttt tggataaggt ctaaagatag atattgtaga
     2941 gaacccctaa tccatctaat taaatttttt ttaataggtg cttttctaag tgttataatt
     3001 atacttttag aaaatttact gatgaagttt aatgtatttg aagggtattc agaactaata
     3061 tatgtatcat ttgtagttgc aggacttgta gaagaaggtg taaaagctct gatactgatt
     3121 cctgccctaa ttaaagaaaa acactttact gaaaagttag atggtattat ttactctgta
     3181 tttttagctt taggatttgc aacaattgaa aatatggttt atattttttc tgaaagtaga
     3241 aatctagcac tgcaagttgg tattaatagg gcagttatct ctataccagc tcatgtaatg
     3301 tttgcaatta ctatgggcta ctatatttct aagtataaat ttgagggaaa taaaaataaa
     3361 agacgtgagt atttgtttat ggcagtgtta attccaattt tattacatgg agtttttgat
     3421 ttcatattga tgatagaata tagatgggct attatattac ttatagttta tgtaataatt
     3481 ttgtggaaga taaatcttga taaacttgag aaatatatga atcactctaa aaaagtgttt
     3541 ttcggtaatc ttaggaaaaa gaagaagaaa taaaatttaa aggaaagaca taaataatta
     3601 aagtataata aatgacacac aagtaaagca gattgaaggg ggattataga tttgacagaa
     3661 ttaccaaatg aatttttaga agatattgaa gaaatactaa aagatgaata tgatgatttt
     3721 ataaaaagtt atgaggaaag taagactact gggcttagaa taaatacttt aaaaattgac
     3781 aagaaaaaat tatttgattt ggagttgttt gatttgaatc aaataccatg ggcagaagaa
     3841 ggtttttatt atgatgataa aattgacaga cctggtagaa atccactgca tgaggctgga
     3901 gcatactatt tgcaagagcc ttctgcaatg agtgtaatac caaaggcaga tattaaagag
     3961 ggtgaaaaag ttctcgatat gtgtgctgct cctggtggaa aatctacata tatactatct
     4021 aagctaaacg atacaggttt attggtttct aatgaaatta atgctagtag aattaaggct
     4081 ctaggggaaa atttagagcg atttggagcg agaaattgta ttataacaaa caccgattct
     4141 aatagcttaa gaaagacgtt taatgggtat tttgataagg ttattataga tgcaccttgt
     4201 tctgggcaag gtatgtttag gaaggatgag gttgccatat cagattggag ttatgcaaag
     4261 gtagtagaat gtcaatccat ccaaaaagag attataagag atggatataa aatgttaaag
     4321 aaggatggca ttttaattta ctcaacttgt actttttcaa gggaggaaaa tgagcatgtt
     4381 ataaatgagt ttatcagtga atatccacat gcagaactta ttgaaatgga aagattatgg
     4441 cctcataaga taaagggaga aggtcatttt gttgccaaaa taaaaaaatt agaagaagaa
     4501 gaatgtaatg tcaaagaatt aaaaataaaa aatttagata aagaaataaa ggattatagg
     4561 gagtttgaaa agaaattttt aaaggttgat ttttatagta ataaaaaaaa taaattttat
     4621 ttaagaggag aaaatttgta cctacttcct gaaatatatc cagatacgaa aaaactaaaa
     4681 gtattgagat atggtttgca tttaggggtt ttaaagaaga atagatttga gccttctcat
     4741 gcactatctc attacttaaa agttgaagat gtaaaaaatg ttgaaaactt cagtgtacag
     4801 agtgaatcaa ttcttaaata tttaaaaggg gatgttgtaa attctaatga tagtagagga
     4861 tgggttttgg tttcggttga aggaatacct ttaggttggg gaaaagaatc ttctggtgta
     4921 attaagaatc attatcctaa aggtcttaga aaagtatttt aaactattag aaaaaagaag
     4981 tataatattt atattgtagg tttatggtag attattttgc tgtagattga cttaatattt
     5041 aacacagagg agacgatttt agtgggtgaa gcagttaaaa aagaagttgt agaatggata
     5101 aaagtgattg tcatagctct tgttttggca tttgcaataa ctcgttttat agtgccaaca
     5161 atagtcaaag gagaatcaat gtatcctaca ttagttgaac gtgattattt gatagttaac
     5221 agaattgcgt acaaggtagg agagccaaaa tacaaagata taatagtatt caaaaccgac
     5281 ttaacagagg aaaatggaaa gaaaaaagat ttagtaaaaa gagttatagg tgttcctggt
     5341 gaccatgtaa aaatacaaga ctccaaggta tatgtaaatg ataagttgtt agatgagact
     5401 tcctatatac ataataatcg tactgatgga gatattgata tcgtagttcc agaaggaaaa
     5461 ttatttgcaa tgggagataa tagagaaaaa agtttagata gtagatacga tgaggttgga
     5521 ttggtcgacg agcataccat tttaggaaag gttctagtca gattgtatcc attttctaag
     5581 ataggaacta ttgactaaac atataaaatt tggagcaatc gctgattagc tgaaatcacc
     5641 agaccataca caatagttgt ctatataaaa aagacaacac cttatttagt agacttatct
     5701 aaaagatagt gtttaagaga cattatcaat aaagataata taactataaa ggagaatttg
     5761 tgaatgaatg aaactattaa agaagagatt gtagagtgga taaaaataat tattactgca
     5821 cttttttttg catttattat aactcgtttt ataaaaccaa cattagtaaa tggagaatca
     5881 atgtacccaa cacttaaatc acatgattat ttggtagcaa acaggatgac atataagtta
     5941 tcagaaccaa aatgtggaga tataatgata tttaagactg atttattaca agagaatgga
     6001 aggaaaaaag agcttgtaaa aagggttata ggtgttcctg gtgaccatct aaaaattaag
     6061 gattctaagg tttatataaa tggtaagtta ttaaatgaag tttcatatat acatgataat
     6121 tatactgaag gcgatattga tatggtgatt cctaagggaa aagtatttgc gatgggagac
     6181 aatagagaag ttagtttaga cagtagatat aaagaagtgg gattagtaga tgaagaaaat
     6241 attaaaggaa aagttatttt aagagtattt ccttttacag atataggtat ttttgagtag
     6301 gtgatttgta tggcagttgg tatagcgtcc ttattgttta atattgagga ggctttaaac
     6361 atttgtgaga gtataaagca aataactcat ttagagattg gaattgacaa tatatctgaa
     6421 tgtagtgagt tatgtaaata taaagagaga atttctaagt taggattatc tatagggata
     6481 cacttgccta tggaattaaa tacttgcgaa aacatagagt atatacgaaa ttcatggata
     6541 aattttattg agaagataga atttgaacta aaaggatttg atttaaggta ttttaacctt
     6601 catttgggat atgttatgac aaatagagtt agaaagaata gagacaaata tttaggaaat
     6661 agtgtggatt ttttagataa attaaataca aattcatatg tatgtataga gaatacttat
     6721 tcaaaaggtg gggatttttc aaatattgga aatatatctt atgattttga atatatattt
     6781 aagaggatta aaaattctaa gatttgtttt tgttacgata ctggacactg tttaattgat
     6841 gaggatgctt atgtaaagaa cttaaaggat aaaataagac tgatacactt aagtgataat
     6901 gatggtatca atgatacaca tgttggtata ggaagaggaa ttttatctga agaggggatt
     6961 aaagaggtct taacattaga tgcagaatat ttagtattag aaataaatta cgaagacatt
     7021 gaagatacta taagtaaatt gaataatatt gtaggagagg gttaaatacc tctctttatc
     7081 ttgttgatat gcctcattaa ttaaaattta tattcatcaa aaggaaggag atgttgaagt
     7141 gaaaaaaata aacttaaaaa tagaaggaaa agataaggaa tattcattag aagaaaattc
     7201 ccctggaatc agattgggag atatagcaaa agaattttgt gatgagcata agggttatat
     7261 aacacttgca gttgtagaca ataaactaaa agagctaaat tgtagagtta agaaggattg
     7321 tgagataaat tttttagata ctactaatga agatggagag agagtttact ttagagtcat
     7381 gtcatttata tttgtaatgg catgtagaga aatcttttgg gatagcagag ttacaataga
     7441 acattcacta tcagatgggc tatattgtga agttcatata gatagaaagc ttaaagaagc
     7501 agatgttgaa aatataaaaa acaagatgaa agagatagta aacaacgatt atgttataga
     7561 aaaaattgaa gttacaagaa atgaagctat ttctatcttt gaaaataatg aaatgtatga
     7621 aaaggcagag cttttgaaat acaaagagta tgatagtgct aaagtatata agtgtagaaa
     7681 ctatatagac catttttatg gttatatgtt gccatcaact ggttatataa aatcatttga
     7741 tattaagctt tataatggag gcgttataat tttaggacct agtgaagaag ataagactct
     7801 tcctatgaaa tttgtacctc aacctaagct atcaagtgta tattatgaag cagaagaatg
     7861 gtcaagactt atgggaattg acaaggtagt atctcttaat aaaataatag aaaacaatga
     7921 atatggagat ataataagga catttgaagc attgcatgaa aagaagctat ctcaaatagc
     7981 ggatatgata aaggatggca ataagagagt agttttaata gcagctccat cttcatctgg
     8041 aaaaacaagt tttgctcaca gattatcaat acatcttaga gtaaataatt taaatcctat
     8101 ttcaatttct ttagatgatt attttataga tagaaaacat actcctttag atgaatatgg
     8161 aaattatgat tttgaatcaa tttatgcaat agatttagag aagtttaact tagacttaaa
     8221 gaaactttta gctggtgaag aaattgatga tataagattc aattttaaag aaggtaagag
     8281 agaatacact ggtaaaaaga taaagataga ttcaaatcag ccaattatat tagaaggtat
     8341 acatgggcta aatcctattt taacatcatc tataccagat gaagataaat ttaagatata
     8401 cataagtgcc cttactcaaa taaatttgga tgaccataat cgaatcccta ctgctgattt
     8461 gagaatgata agaagaattg ttagagacta taattttaga ggatatagtg ctgataatac
     8521 tattttacaa tgggcatcag ttagaagagg agaaaagaag aatatattcc cgtaccaaga
     8581 ggaggcagat gctatattta actctgcttg tgtatatgaa ttggcagttt taaaacgatt
     8641 tgcgaaacct ttgttggaag agataaagga agataatcct gcacatattg aagctactag
     8701 attactaaag tttttgcagt attttgttga attggacgat acttctgaca taccaggtat
     8761 ttcaattctt agggaattta ttggaggaag taaaatagtt gattaatgtg tgtattatag
     8821 attgttttat gaagattaaa tggcatagca agtagtgtta ttggtttatg ttaagtatat
     8881 taagtgtaaa gtggagaatt aaattgatac agattttaaa gtttgagttg atatataaaa
     8941 aggcttgttt tatacttttg tatgaagcaa gctttttatt ttaaatttta cttttttgtt
     9001 taatattttg ttagtttatg tgattatttt ttaaaagagt aaaagagtaa aagagtaaaa
     9061 gagtaaaaga gtaaaagagt aaaagagtaa aagagtaaaa gagtaaaaga gtaaaagagt
     9121 aaaaggtgtt gggttagaaa tttttggtgt tataaaaatt taaattactg gattattaga
     9181 ttgctggatt gctaaattac taggttgtta aattattgaa ttactaaatt attaagctct
     9241 ttaaactatt taaattgttt gaaaaatgaa ttgattcttg tagaaaataa ttttaaagta
     9301 taattttttt taacattgtt ttattatagt ataaacttgt gctaaaatag aatatataac
     9361 ataatataag aggtgcatat gaatacgtta gatgagacat tattaaaaga aattataagt
     9421 tctaataaga agtatactaa ttatggacaa gtcgctagtt acatcccaga attaaaaaac
     9481 gctagaagaa atgatttagg aatttgtatc atagatagcg aaaataatct ttatagtgct
     9541 ggaaactgta gcacaaagtt tactatacaa agtatttcaa aaccaattgt gcttgctatg
     9601 gcacttatgg ataatgattg ggaagacgtt ttttcaaacg ttggaatgga gccaagtggg
     9661 gacccattta attcaataat gaaacttgaa attaatgaca caaaaaaacc ctgtaatcca
     9721 atgataaatg caggggctat agtaaccact tcccttataa atgggagttg tttagaagaa
     9781 aaagaagaaa gaatgcttag tttctttaga aagttagcaa aaaatgataa tataggaata
     9841 aactatgatg tctataagtc tgaaaaaatg actggagata gaaacagagc tatggcttac
     9901 ttattgaaaa gtgatggatt tatcagaggg aatgtagaag atgttctaga cctttatttt
     9961 aaacaatgct caattgagat agattctgta gatttagcga gaattggaat aaatcttgca
    10021 aattatggag tagatataga aaatggtgag catttaatga gtgaaatggt ttctaggatt
    10081 gtcaagacat ttatgatgac ttgtggaatg tatgatgctt ctggggaatt tgctattaaa
    10141 gttggcattc ctgcaaaatc tggagttggg ggtggtataa tggcttctgt gccgggacgc
    10201 atggggattg gtgtttatgg acctgcgctt gacaaaaaag gtaacagcgt cgcaggagtt
    10261 aaagttttag aggagttgtc aaataaactc aaattaaata ttttttaaaa tattaagaaa
    10321 ggattttttt gacaacaaca tataatttaa tatataatta aattgtaaag aaatatgaat
    10381 taaataaatt ttttaggagg ggtcaatgaa agaggtattg gtattacgag atttggagtg
    10441 cataaaggct attgcgcatc cacgtagaat agatattcta aaagcatttg acaaatcgcc
    10501 attgtcagca aaacaattat ctcaattatt ggaagaacct catgcaaaaa taaactacca
    10561 tataaaaatg ttatacaaag ttggtatttt agaattagtt gaggaaaaaa taaagtcggg
    10621 gattgtggaa aagtactatt atccaagtgc aaaaaatgtg gttataggga ataggatact
    10681 taatttttct ttagacaatg gggaagaaaa agaagaattg tacatatcaa aatttgaaaa
    10741 tatgagtgaa gttttttata aggcagcaga ggaagatgtt ttagaaaatg aaaatatagt
    10801 tgattatcat gatatttctc ttactcatga tgaattagtt gaacttagtg acactatgaa
    10861 gtcaaagata gatgaaattt taaataaaag acaacacaat gttgaaggtt ctaagtatga
    10921 tatagctatg gttatagttc ctactcttga ggaggaatgt cctagttaga gaattaatta
    10981 tggattaatt aaagatataa tattagagat tgaacatcaa aatttactta ataatcaaaa
    11041 aatagttttt ggtatgggta aaacaatgat ttttaatctc tatttttttg tttttttata
    11101 cctattgtat ttgtaaataa tatgtatagt gtaagatgta tacactatct aagaagtgct
    11161 tagataatat acaaagtatg taagattata atatttgaaa ttgattaaaa tgggtataat
    11221 taaagtgtaa atattaaaat taaaaattaa tataaattaa atcaatgcta gaaatatgtt
    11281 aaaagaggac tgagattagt tgggatagaa catcccttag agcttacgaa tttttagtga
    11341 aaaagtacta tcaaatagga atctccatct tcatatgttg gctaaatgta gttaaggtgg
    11401 agtagttcaa aattaaaatt gagtacaagt agtagtacaa ataaaaggag attaatatga
    11461 ttaaattagg aacacatctt tcaatagcaa aaggatttgc taatgcggct gagacagctg
    11521 tatatattgg tgcaaataca tttcaatttt ttagtagaaa tccaagagga ggaaatgcta
    11581 aagaatttga tgaaaaagat ataattaaat ttcaagaaat tagaaaagaa aataattttg
    11641 caccactgct tgcacatgca ccatatacta tgaatctggg tggtacaaag gatgatgtat
    11701 acgaatttgc aacaagggtg atagatgagg atataagaag gatggactca ttaggaattg
    11761 aatacatgtg ttttcatcct ggaagtcatg taggtggtgg tgtagatttt ggcatagata
    11821 gaatagtaaa aggattgaac aatgccataa agggtgatga aaatataact atacttcttg
    11881 aaactatgtc gggaaaggga actgaaatag gttttaaatt tgagcaatta aaaagtataa
    11941 tagacaaaat tgagcataaa gaaaaaatag gtgtgtgtct tgatacatgt catatttttt
    12001 cagctggtta tgatatagtg aatgatttag atggggtttt ggaagaattt gataaggtta
    12061 taggcataga aagattgaaa actgtccatc taaatgatag tatgatgcca tttggagata
    12121 aaaaagacag acatgctcct ataggggaag gaaagatagg attaaaagca ttaattgatt
    12181 ttatggaaca tcctagtcta aaacatttac catttttctt agaaactcct tttgatgatg
    12241 aaggtcacaa gaaagagctt aaaatgataa aagaaatact atctagtaaa taaaccttta
    12301 ttaaaaatat gtgaaatact tcaccctcaa cataattagg gtggagatac aatcatgaaa
    12361 tgtgataatt taaattaatg gataggaata acaaaaattg tatacaaatt aactgggtat
    12421 aaagtatgat tatattatga atagactgat ataaaatata attatactat gaatagtatg
    12481 taaatatatt gcaaagattc tcataatttg ttgtataatt aaagatgaca tatttatatt
    12541 atataaaaaa ttattaaaag ttaggtgggg aaagatatga gaaatttagg caatactaac
    12601 atgaaaataa agagagttgg ctttgggggc attccaatac aaaggattac tcaagatgat
    12661 actaacttag ttataaatga gttagaaaaa caaggaataa actttattga tagtgcaaga
    12721 gggtatacaa taagtgagga agcaatcgga attgccattg aaggaaaaag agataaattt
    12781 ttcttagcta ctaaaagtat gtccagagat tatgattcaa tgaagagaga tgttgaaata
    12841 agtttaaata attttaaaac tgattttata gatttatatc aattccataa tgttaaagaa
    12901 gaggagtatg ataatctttt taaagataaa atggcttata gtgccctttt agaggcaaag
    12961 gagcaaggta agataaaaca tattggtata actagtcaca atttaaatac tatagaaaaa
    13021 gctatagaag atggaaaatt tgacactata caattccctt acaatatagt tgaaggacaa
    13081 gcagatgaag tgtttaaaaa agctcatgaa aaaggaattg ggataatagt aatgaaacct
    13141 ttagctggag gtgctttaga caatgcaaca ctggctatta aatatatact atctaaagat
    13201 tacatagatg tagttatacc aggtatggag agtgttgaac aggttagaca aaatgtcgct
    13261 gtacttgaaa atcttgtgct agatgaaaaa gataataagg aaattgaaga aataagaaat
    13321 tctttaggta aaaaattctg tagaagatgc gaatattgta tgccttgtgc tgtaggaata
    13381 aatataccat tgagtttttt atgtgaaggt tattatacta gatatggatt aaaagagtgg
    13441 gctaaagaaa aatatgaggt tatggatgtt aaacctactg agtgtattga ctgtgggctt
    13501 tgcgagagta gatgtccata tgaacttcct ataagagaaa tgcttaagac tgttgttgaa
    13561 aagctaggat agattttatt tattgaaatc ggtgtattaa atttactccg atttcttttt
    13621 attagatgtg gtcttatata caaatattaa atttacatag ttatagctat tatataaatt
    13681 taatatttgt ttaatactta ttaaatattt acttaatgct ttttaaaaac atttgtttga
    13741 gtatagacat aacacaatta ttattgttgt attagtatag tttcaaatac tggagtatca
    13801 atataatgtc gaataacaca taaaatcttt atttttcaaa aatattatag gatatatcgt
    13861 atttagatac tggataaagt tcaatttcaa ccctagggtt atctttatct ataaaatcaa
    13921 ataaaagtac tggcttaagt tgtttatcat tttttattat tccacttttt tcaataccat
    13981 cacaaatact ttttggataa ttatgtaaat cccccattct cttatttggg aagaataatt
    14041 tcaagactgt tattagattt tcttcaacag tttccccttg atactgttgg tttataaaac
    14101 ctgcaatcat gttttcgtat gcaagatact tagaatattt tccctttgaa ggcatccatg
    14161 cttgaccatt tatgttatga agcttaaaat tagatttact aataggtcta cctggaattg
    14221 ttattttaat caaatttttc accatcctta tacagtattt attataatct gtatatgatt
    14281 taaaagcaaa ttgttattta aagtgttttt acagagcttg tttgtaaaat tttccacttt
    14341 agatgtagta atataagata agtattttgc ttacaaattt taaaatttga tatatgatgg
    14401 gcatattcat attagactat tataagacct tcataatatt aataagtata agtttagtaa
    14461 agagagtaaa aagaaatcca tataagtcaa gtaaagactt aataaatggc aatgtaaatg
    14521 ggatattaat tataagaaaa aatcaatctt tttgcttaac tatagaaaga atgtcaaaat
    14581 ataactaatg atatttgaag ttttagagtt tatattaaaa ggtaagaagg ttaagtgggt
    14641 aaaaaaggtt gaatagatta aaaaggctaa gtatctaaaa gagttaagta ggtaaaaaag
    14701 ttaaataagt aaatgaaaaa ggctaagtat ctaaaagagt tagtaggtaa aaaagttaaa
    14761 taagtaaata aaaaagatga agtatctaaa agagttagta ggtaaaaaac taaataggta
    14821 aaaagatgaa gtatctaaaa gagttagtag gtaaaaaatt aaataggtaa aaagacgaag
    14881 tacctaaaag agttagtagg taaaaaaatt aaatagataa aaagacgaag tatctaaaag
    14941 agttaagcag gtaagaaaat tgaatagcta aaaagattaa gtatttaaaa gagttagtag
    15001 ctagaaaagg ttaaaaagac taaaaaaatt aaataaattt aaaaagtcaa ttagattaaa
    15061 aaggaataat tttatggata ataaggaatt aaaagcattt tgtaatttaa taggattaaa
    15121 ctgtgtagga gtagcagggg tagataaata tgataattta gaaaagatac ttaagaacag
    15181 acaacaaaaa ggttatttaa ctggtatgga agaaccaata attgagagca gaattaaccc
    15241 aagacatata atgaaggatg ctaactctat aatagtctgt gcatttccat atcatataaa
    15301 tagacaagaa aagaataaaa attcaaattt atcaaaatat tgtcatggaa aggattatca
    15361 cattgtagta aaggactttt tacaacaaat ttgtgattat atttctaaaa cagtaacaga
    15421 ttttaaatac aagttatttg ctgataatgg accattagtt gacaggtatt tagcttattt
    15481 atcaggaata ggatattttg gaataaataa taatatcata actgatgaat atggttctta
    15541 cgtatttata gggtatatag taaataatta taaatttgaa gaagataaac cactagaaaa
    15601 aagttgtttt aaatgtggta agtgtgtaaa atattgtccg ggaaatgcta tattgggaaa
    15661 ttatgatatg aaccccaaaa aatgtttatc atatataact caaaaaaaag gagatttgtc
    15721 agagaaagaa aaagttgttt taaagaatgg caaaaaggta tttggatgcg atatttgtca
    15781 agaagtatgt ccacataatg ttgaaatacc aacaactcat attttggaat ttaaagaaaa
    15841 tataatagat tatttggatt atgatgagat acaaaatatt tcaaataaag aatttaaaag
    15901 gagatatgga gatagagctt ttagctggag aggtagaaat ataataaaga gaaatatgga
    15961 aataatttta gataagtatg atgactaata ataagagtct gtttcatata ctttaatgtg
    16021 tatttacttt aacacttgtt gtgtaaaagt catattatat cattaaagtt aaatatgggg
    16081 aggtggggga atattgaaat ataataatac atctgaaata aatccactag attatatatt
    16141 gggtcaagaa agagctgtag aagctatgga attagggttg aaaataaata atccagcgta
    16201 caacatatat atagctggtg aacctggaac tggtaaaagt acttatgcct taaaggtttt
    16261 aaatgagtat gcatccaaaa aaaatacgca taaagattgg tgttatatat ataattttga
    16321 aaaccctaga gagcctatta tagttgaatt agaaaagggg tttggaagag aacttaagaa
    16381 agatatggag aaattaattg aaagtctttt agaagatttt aaaaatgcgt ttgaaagtga
    16441 aaattttgaa atagaaaaaa ataaacttct ggatgaatat gaaatagaaa aagatatgct
    16501 tttgaagcaa attaaaaaat atggagaaga aaaaggattt aaattaaaac agagtaaaat
    16561 gggaatagtt tttatccctt tgaaagaaga ggaagatgaa agtgatgagt ctgatgaaga
    16621 gttttacaaa gctaaaagag aactagaaaa tatggcgata caggtcgtat ataaaattag
    16681 aaacttagaa gaaatagctg aaaaagcagt gttagagctt gaagaagaga tagctaaact
    16741 tgtagtagaa cctcatatca agagattgtg tgaaaaatat gaaaattatg ataagattca
    16801 aacttatctg gaaaatataa agaaagatat aatagaatat atgtatttat tctatttaga
    16861 tgaagaggag ttaaaagata agtatgataa agagcatttt attaaatata aaattaacct
    16921 atttgtagac aatggaagct caaagaataa agaatcagca cctgttgtag tagagataaa
    16981 cccaagtcca gcaaatctat ttggaaaggc agagtatgat tatgcaaatg gaaatataaa
    17041 gactgatttc acaaaactat tatcaggagc atttcatagg gctaatggtg gttatttagt
    17101 actttatgca gaccaattac taaaatatac aatgtcttgg gaaattctta aaaagacttt
    17161 gcagactaaa aaagtaatat tggaaacaca aacagcaatt aaaccagaaa atatgccatt
    17221 ggatgttaaa ttagtactga ttggaagcca ctatatatat gatattttat atagatatga
    17281 tgaagatttt gaaaaatatt ttaaagtatt tgtagacttt gatagtgaaa tggataaaaa
    17341 tgaagataat gaagaaggaa tagcaagatt tatagcatac caatgtgata agaataattt
    17401 aaggcatttt actaaatcag cagttgaaga ggttattaaa tttagcacta gattatctgg
    17461 tgatttagaa aaattatcaa ctaagtttaa taaaataatg gaagttgtaa tagaaggaag
    17521 tgcttatgca gagtttagaa attcggagta tacaaagcag tgtgatgtta aaaaagcaat
    17581 aagtgaaaag agaaaaagaa tcaatagagt ggaaacacat atggatgaat caattgaaaa
    17641 tggatttact ctaatagaaa cagagggaag aaaagtagga gtgataaatg gtttgtcagt
    17701 cttaagtaca ggagaatact cttttggtag agcttcaagg attacagcga ccactagccc
    17761 aggaagtaaa ggcatagtaa atatagaaag agaagtaaat atgagtggct ctatacataa
    17821 caaaggagta cttatattag gtggctatct ttctgagaat tttgcacagg atttactatt
    17881 gtcactaaat gcatatgttt gttttgaaca aaattatggt ggaatagatg gagatagtgc
    17941 atcttctgca gaattatatg tactattatc atctcttagt ggagtatcaa ttaaacaaaa
    18001 tattgctgtt acaggttcta taaatcaaaa gggtgatata caagtagttg gtggtataag
    18061 tgaaaagata gaaggatttt attctatatg taaaactaaa gggttaaatg gtgagcaagg
    18121 cgtcataatt cctagaaaaa atcaaagaaa tctcgtttta tcagatgaag ttaatgatgc
    18181 agtgcgagat ggaaaattta agatatatac tgttgaaaga gtagaagagg ctatagagat
    18241 attgacagat gttaaatttg aagaaataaa gaaattggta aaagaaaaat taatatcttt
    18301 cagcaagata caaactgtgt caaaagaata agtttattat agaatttaat gggtatttat
    18361 gtataataaa aagcaagaac tttaataggt ttttgctttt tttgtaaata tatataaatt
    18421 aaattcttat tttgtatgtt ttatatattg atgtttattt ttttgttgat atgattaaac
    18481 tatttataaa tagcgtttta taaattggta ttttgaaggg ggataattat gaaaaatgta
    18541 aatctaaaag agataaatga aaaagatgta attttggaag aagagataaa taaaaaagga
    18601 atggataaga aaaatataat tgagaaggaa gagataaata aaaaaggaat agataagaaa
    18661 gatataattg agaaggaaga gataaataaa aaaggaatag ataagaaaga tataattgag
    18721 aagaaagaga taaataaaaa aggaatagat aagaaagata taattgagaa gaaagagata
    18781 aataaaaaag aaatggataa gaaagatata attgagaagg aagagataaa taaaaaagga
    18841 atgaataaga aagatataat tgagaaggaa gagataaatg aaaaagaaat agataaaatg
    18901 attggtaaaa aagatgagat aattgatgag aaagatatag agaattttat ggatattgaa
    18961 gaagataaaa aaagtatcaa agtaagtaaa aaaagtacta aaaaaggtga aacggagaaa
    19021 aaaacaaaga agagtagttc taataaggct acacaaaaaa caagtaaaga aactaaaagt
    19081 aagtttaaaa ttaaaaatga gtcagcaaat gaaaaagacg taaataagat tttagacgag
    19141 ttagaaaaac tgtacccaga tgctaaatgt gagttaaatt atgggacagc ctttgaactt
    19201 ttaatagcaa ctattttatc agcacaatgt actgatgtaa gagtgaataa agttacttca
    19261 gagttgttta aaaagtacaa cacagctaga gactttgcta atttgagcat tgaagaaatt
    19321 agtaaagaga taaagagttg tggtctttac aaaagcaagt ctcaaaaaat aaaggataca
    19381 tctgaacagc tatgtgaatt atatgatggt gaagtacctg atagcttaga aaagcttata
    19441 aaattacctg gtgttggaag aaaaacggca ggggttgtac ttagtaacgc ttttaatcat
    19501 ccagcaatag ctgtagatac tcatgtattt agagttagca atagaatagg aatagtggac
    19561 gagccaaatc ctcaaaaaac agaatttgca cttatggaag ctataccaaa agaaagatgg
    19621 tcacactcac atcatgtatt aatttttcat ggaagaagaa tgtgtaaagc tagaaatcca
    19681 gaatgtgcaa gttgcccaat aaaagaagat tgtaattatt ataaagaact taatgaaacg
    19741 aaataacttt tagaaagtaa gttgtacaag agggattttc tgatataata atacttgtga
    19801 aaatgttaga taaaaaacta ttatatacat gaaataaaaa ttattattaa cattatttta
    19861 tataatagta taatattatt gttattctgt ttataaaata aaatttgata aataaaagcg
    19921 atacttaggg aggattaaaa tgtcattaaa tatagtatta gtagaaccag aaatacctca
    19981 aaatacagga aatataataa gaacctgtgc agcaacaggt tcaactttac atttagtaaa
    20041 accactgggt ttttcattgg atgataaaca cctaaaaaga tgtgggcttg actattggga
    20101 tatagctgat atacaatatt acgatagctt tgaagaatta caagaaaagt atccaaatag
    20161 taaatacttt ttttctacaa caaaggctaa acagtcacat tcagatgtga aatatgaaga
    20221 aaattgtttt atagtatttg ggaaagaaac aaagggatta ccagaacctt tgttgaaagc
    20281 aaatatggat acttgtatta gagtgcctat gttggatgtt gaaaaagcta ggtcattaaa
    20341 tttatcaaat tcagttgcta ttgtagtgta cgaggcttta agacaaatag gatacccaaa
    20401 tatgaggtag tttcaacaag cctaaatgag aaaggagcat acttaatgaa aaatctaaag
    20461 ataatctgtg acagtttatc ggacgtgcca aaagatttgt tagataaata tgatatagat
    20521 atagtttcat taacagttat actggaggaa agagaataca aggatagaat tgatatatct
    20581 ggagatgaat tttataaaaa gcttagagaa gaaaatgtat atccaaaaac ttctcaagct
    20641 acttatgccc aatttaagga aatttttgag aaatatacaa atgaggatag agagatactt
    20701 tatatagcag ggtctgccac agctacaggt acgtatcaaa gtgctgtaat ggcaaagaat
    20761 gatgtagatg gtaaaatata tacatatgac tctaacttgc tttgtatggg aactggttta
    20821 cttgtaataa aagcaggaga acttgcaagt gaaggaaagt ctgttgctga aatattgcct
    20881 gttttggatg aattaaaaga gaaggctttt gttatatttt cagtagatac attggaatat
    20941 ttacaaaagg gaggtagaat atcatctact aaggcagcaa taggaagtat attaaatata
    21001 aaaccgattc ttgaaataag agatggatta gtttcgcaaa tatctcaagt aagaggaaag
    21061 aagaatgtaa tttctaagat gatggaattg ataaaatcaa attgtggtga tgatttatca
    21121 gaccaaattg tatatatagg atatagtgat gattttaagg aaaaagaaca attatctaag
    21181 gttatgcaag atgaattaag acctaaagaa attagatttt tccaaattgg tgtgtgtata
    21241 ggtgcacatg ctggtcctgg tgttactggt atcatatgct taaaaaataa ataatatatt
    21301 tttggatgac attatttatt tttttattaa attaggatag aatattaatt attgagatgt
    21361 taaattggat ttattttgga tgaaattaag tgttgatata ttagatttag tttgtaggga
    21421 agtgtgtaaa tagataagaa tctattgcac acttttttat agtaagattt attaaaaaaa
    21481 tttcaaaatg tacttgtaaa ttgtatataa aaaaattata aataggatgt aaaagtaaat
    21541 aggatgtaaa agtaaatagg atataaaaat atcgtaaatg tatgaggagt gaaatttaat
    21601 gcgtttcaaa agaatattga tattgatgtt aactgtagca atgatagctg gaatgttggt
    21661 tgggtgtgct aataacccaa gcaataatcc tagctctagc aaagataata aaagtgatat
    21721 aagcgataaa agtaattcaa aaaaatctgt atcaatgata ctagatattg aaggaacaaa
    21781 taatgaagct atgaataatt cagctttatt agcattaaac aatgctcaaa agaaacttaa
    21841 tatagataca aacaaagtgg aatcagatga ttcctcaact ttttctaata gtatagatat
    21901 tttatgtaat gacaattacg atttaattat agctgtagga gctagatttg caaaaccatt
    21961 ggagatggta gccaagaaat atccaaagca acaatttgca attatagact atgagtatga
    22021 caagcaacct tctaatataa cttcaatttc ttatgaagat aataaatctg gatatctagc
    22081 tggattaatt gctggaaaaa tgactgaaag cgataaggtt ggttttatag gaggaacaaa
    22141 gagttctagt agagataaat ttgaatcagg ttttagagag ggtgttaaat tttctaatag
    22201 tagtataaag gatatttcag ttgagtatgc agatgttttt aaagatagca aatcagtaga
    22261 atctatagct aaaaaaatga tggataatgg agtggatatt attttttcta ctactgaaga
    22321 tgattcaaaa gctgtaatag atgcagtaag ggcaaaaaat aaaaaagtta tagcaacgaa
    22381 taaagaccaa catgagttag ctccagaaaa tgttataagt tctattgtaa aagattttga
    22441 gaatcctact tataatctga tacaaagttt tgtaaaagga aattataaag gcggaaaagt
    22501 tatagaaaac acagttaagt ctggaagtac tgggcttgcc caaaatagtt ctcaaaatat
    22561 tcctccagat gtgttagaat atgttaataa gaacaataaa taatctacta taatatccaa
    22621 gaatacttat gactttagtt atgaggtgaa ttggatgtta taatagacat atatagaaag
    22681 acatatgcag aggtaggtca gacctatcta aatcagggag tgaattttgt gaaaataaaa
    22741 aggatattga tggcttgctt tgtagtagtt tttacaattt ttatattagc tatatgtaat
    22801 tttgattttg ggtttgataa agtaaataag aataattcta atatagatag tgctaaatcg
    22861 aaacagagtg agtctaaaag tttgccaact tcagcagaaa aggataaatc aaatagtaaa
    22921 agtaagaata aagaagatga cacagaagat gattcatatg taagaaaaac tattggagta
    22981 ttgttagata ccgaaaactt aaataacgaa tcatataata acaatgtact atcagcctta
    23041 aaaagggctg acgaagattt tgatgttagg ataaaacttg aaaaaccaaa gacttctaaa
    23101 agttttaaag agagtataga aaaactgcat aagtatgaac cagatttgat aatagcagtt
    23161 ggagccagat ttgcagagcc aattaaagaa gctgctactg aatatcccaa acagcaattt
    23221 gcaatagttg actatactta taaagttcag ccagctaatg taatttctgt atcatttcaa
    23281 gataatgtta ctggatattt agcaggactt attgcaggta aaatgactga tactgggaag
    23341 gttggtttta taggaggagt tgagggttct agtagagata agtatgaatc tggatttaga
    23401 aaggggctta aaacttcaaa taaagatgct gagttactag ttaagtattc aaatatattt
    23461 gaaaaccctg aatcagctag aactattgca aaggaaatga gagattctga tagagatgtt
    23521 gtattttcag ctacagagga tgatagtaaa agagctattc aggtagcaag agaaaatgga
    23581 ggtaaagtta tagctattaa taaagaccaa tacgatatgg ctccagataa tgtaataagt
    23641 tcaattataa aagatgtaga acaacctgta tatgaactga taaagaacgt tgcaaaagat
    23701 ggttttaagg gaagtaaagt aatggaattt aaaataaaag atggagaact tggcttgact
    23761 tcaaagagca gtagaaatat accaccagat gttttggaat acataaaaaa agagtataac
    23821 aaagtgaaag atacatatta attattgatg tagaagatta taaaaatata ctgcttaagt
    23881 ttagattggt gataaaagga tatatttatt aaatttgtgc taactgtaca aaatatattg
    23941 attaagttta gattagtgat aaaaagatat atttattaaa tttgtgttaa cagtatggaa
    24001 tatactgctt aagtttagat tggtgataaa aggatatatt tattaaattt agtctaactg
    24061 tacaaaatat attgattgag tttagattag caataaaaag atatatttat taaatttgtg
    24121 ctaactgtac ggaatatact gcttaagttt agattagtga taaaaagata tatttattaa
    24181 atttgaacca actgtacaaa gctatactaa ttaagcttag attaactata taaaattata
    24241 acaataagtg tgtgggcaga aaaattctgc tacacacttt atttatattc acataaaaaa
    24301 tttatcaaaa aaaagaaaaa ggagtaaaaa atggaattta caagagaacc atttatacta
    24361 gatgacgatt tgtgtctagc tttggtggac aaaagaatta gttatctaat ggaaaatgaa
    24421 ttaaaattaa gaaatataga gataataaaa acaatagaat gtaaagaatt atatgaaagc
    24481 ataaaatatc acccggatat atgtatttgt aatttaggaa aaggagatat aattgttgcg
    24541 ccgaatgtat ataatcaata caaagagtta ttaagtagat ataaatttaa tataattaag
    24601 ggagaagcta atttattaaa tagataccct tatgatatac cttacaatat tgcgatagtt
    24661 ggtgactatg ctatacacaa ttttaagtat actgacaaag caatattaga ttatattgaa
    24721 aataataaac ttaaaaagat aaatatagag cagggctatt ctaaatgctc aatctgtata
    24781 gtagatagaa cgtctataat aacatcagat aaaggtattt ggaaaagtat gaagaataca
    24841 gaaatagagt gtttattgat agaaaaaggt cacataaatt tatttgagat gaattatgga
    24901 tttataggtg gatgtacagg gttgatttct aaggataaac tagctttttg tggagatgtt
    24961 aaaaaacatc ctgactatga acgtataaaa agttttgtag aaagtaaaaa caaggagata
    25021 gttacactta gctgtgaaaa tttactagat ataggttcta tagttcctct catgacaaga
    25081 aaggagcgat aactttggtt gagaaggata atatttcttc catgaaattt ttgaggaata
    25141 ataatgtaaa cgatagatta atatatacga atgacattgt agaagtagtg atagattctg
    25201 caaagcaaga agtgttaaat aaaaaaatac ttaatggtac taaagctgaa caaagagctt
    25261 taaatattga tgagatatca acggaaataa ctagtatgat aataagtata atgaaagaga
    25321 aacttttgaa agaatacatt ctaaaatgct atgggcgtac atatcctgaa gataaaaata
    25381 atatatacat atattcactg aacatatttg ctaaaaaaga gatttttatg agagaaacag
    25441 tatttactag ggtgagaaat tatatagaag aaaatgattt tataaatgta gaaggtttta
    25501 ttagatttag aatgagagaa tttatgaaat atatttctgc tataggtgat attgcagttg
    25561 aggagtattt aataaagaaa gaccaagatg aatttataag agttttaaaa tactttatag
    25621 atactcaaga agaaaagatt gatttgctta aagttcatat aatggaagat aatacatttg
    25681 ttttatacga taaaaatgga aataagatag acagtataga tgatgaagaa attataaata
    25741 tggtaataag agaaaattta aattatgagg actttttaat aagtacctta ttaactctat
    25801 gtcccaaaaa aatagaaata ttggatttgt taaataataa ttgctcaaag gaaattatag
    25861 atactgtaga agcaattttt gaaaataaag ttagtataat tatggaaaac tagaatctaa
    25921 gcatataaaa gctatccata taaaattaaa aaaattcttt ttaaatcgac acaaaagact
    25981 tataattgta tataaagcta tagtataact agtgttatta agtataaaga atatctactt
    26041 gattaataca atatagctgg caaatataca attgataagg agtaatttta taatggataa
    26101 tttaattagg atactgatat tagctgttat tggtggattt ataggttatg ttacgaatgt
    26161 agttgctata agactgatat ttagaccaat agagcctatt aaaataccta ttttaaatat
    26221 agaaatagta ggtcttatac caaagagaag agcagaaata gcagcaaatg taggagaaat
    26281 aattcaagaa gaatttcttt ctatggacga aatacttgca aatatcatta ctgacgaaga
    26341 taaggaagaa gttgtaagat acataaaagc aagggtaaaa attataatac atgagaaagt
    26401 ttcatttatc ccgagtggta taaaaaacat gatacaagat tatctaggag agataattga
    26461 gtctgaagtt aagcaaagta tagatgaatt gagtaaaaat ataataaata aagcaaatga
    26521 acgtatagat atacaaaaaa tggtcgaaga taagattaat gaactagatt tatatgaatt
    26581 ggaagagata ataataagaa tagctaagaa agaattaaaa catatagagt ttttggggct
    26641 tgtcttaggg tttttaatag gtatagctca agggttaatt actatgttta tttaaatata
    26701 tgagaagtag catttattta agtactaatt atatttacct atttgagtga attacttatt
    26761 aatagcattt agatattaat tgtatttatt tatttgattg tgttacttat taatagtatt
    26821 taagtattta ctgtactact tattaactat attaagtatt gattgtattt aagtatacaa
    26881 aacctaaaaa gagtacaaaa attagaagtt agttttaata aaaattagtt ttagtattta
    26941 tctagtcata tctatcatgc atgtggatac gttttaaaaa ttttaaattt atgaaaatat
    27001 tataatatca ctataaatca agagattaat ttaatataaa aatataagaa tattggttta
    27061 cagctgattg agatgaaaaa taatgggtaa ttaatatatt acattaatgt tgattgatgt
    27121 ataactatct gttataatga aaatatatta aaaatcatag tgttgaagag gaagagtaga
    27181 gtagaaacca ttctcagaga atttgggtct atggctgaga gcccatgtaa taggtctatt
    27241 tgaaaactac cttggagctg agcttattag cagtatttgt tttgtactat acgtgaaagt
    27301 gctcgggtga cgaccttata gtcatcaaga gggttgtata tacttgtata cgccaacttg
    27361 ggtggaaccg cgaattaata tcgtcccaaa catatttggg gcgatttttt attacaaaaa
    27421 atctaaaaat tatttcaata aggaggaaaa aacaatgatt aaagttgctt taaaagatgg
    27481 ctcaataaaa gaatttgaaa atgctatttc tgttatggat gtagctaaat ccattagtga
    27541 aggattagct agaaatgtgg tagcagcgtc tgtgaacgga gaggttgtag gtctggacca
    27601 cattatagat actgattgtg acttgaattt atttaagttt gaagataaag aaggtaagga
    27661 agtttttaga catacatcag cacatatatt agctcaagct ataaaaagac tttacccaga
    27721 agcaaagctt gctataggac caagtataga aaatggattc tactatgata tagatttaga
    27781 ccatagatta gttccagagg acctagagaa aatagaagct gaaatgaaaa aaatagctaa
    27841 agaagattta aaaatagaaa gatttgaact tcctagaaat gaagctttag agcttatgaa
    27901 agaacaagga gaagactata aggtagaact tatatctgat ttacctgaaa gtgaaataat
    27961 atctttctac aagcaaggtg attttactga tttatgtaga ggaccacatt taccatctac
    28021 taaaaaggta aaagctgtta aattacaaag tgtagcaggt gcatattggc gtggtgacga
    28081 aaataataag atgcttcaaa gaatatatgg aacttctttt gagaaaaaca aagacttaga
    28141 agagtactta catttactag aagaagctaa aaagagagac cacagaaaat taggtaaaga
    28201 attaggatta tttatgatac ctgaagaagg tccaggattc cctatgtttt taccaaaagg
    28261 tatggaactt aaaaatgaat tattaaaatt ctggagagaa atacacagaa aagctggata
    28321 tattgagata gaatctccaa tcatattaaa tagaaaatta tgggagactt caggacactg
    28381 gtatcattac aaagaaaata tgtatacagt taaaatagat gatgaggatt atgctataaa
    28441 acctatgaac tgtcctggtg gattaattta ttataactct caactacatt catatagaga
    28501 tttcccaatg agagttgcag agcttggtag agttcataga catgagttat ctggtgcatt
    28561 acaaggatta atgagagtta gagcatttac tcaagacgat tcacatatat tcatgttacc
    28621 agagcaaatt aaggacgaaa taaaaggagt agctaactta atagatggta tatacaaaac
    28681 ttttggattt gaatataatt tagaattatc tacaagacca gagaactcta tgggaagcga
    28741 cgaagagtgg gaagcagcag aaaatggatt aagagaagct ttagaagagt taggtctacc
    28801 atatactatt aatgagggtg atggagcatt ctatggtcct aagatagact tccatctaaa
    28861 agattgttta ggaagaactt ggcaatgtgg tactatccaa ttagatatgc agcttccaag
    28921 acaatttgat aatacttata ttggtcaaga tggtgaaaag catagaccag ttatgattca
    28981 tagggttgct tttggtagta tagaaagatt tataggtata ttaatagagc attatgctgg
    29041 taaattccca gtttggttat ctccaactca agttaagata cttcctatat ctgataaatt
    29101 tatggattat gcaaatgaag ttaagaaaga gttatttgat aagggtataa gagttgaatt
    29161 agatgataga gctgaaaaaa ttggatttaa gataagagaa gctcagcttg aaaaagtgcc
    29221 atacatgcta atagtaggtg aaaaagaagt agctgataat aatgtttctg ttagatcaag
    29281 agataaaggt gaaataggta gtattaaatt agatgaattt atagctagta tttcaaaaga
    29341 aattgaatca agagaaagta ttattcaaga ctaatatata ttatattagt agctaagata
    29401 ttgttatata agataagttt aatcaatatg ttgatatgtt gatgcatagt gattaaattt
    29461 atgaaaagat gagtataatt agaggtacag ttaatcatat ttttatgatt aatgtacctc
    29521 tagttttttg tatggtagat ttattaataa atatatcata gaagtgtttt gaacatggaa
    29581 atatttatgt gcattcttta aataaacgtt tattttttga gtgataatta agtgcttgtt
    29641 ggtaaggtta tggctgtaaa aatcataact ttattttcat atttgttcca ttttcataaa
    29701 aattattatc cctttaagaa aatttcttaa tcctcttgaa atatttcctt tttagtctaa
    29761 agttaaattt ctttcttcaa aatgaatatt taaatgaact aaaatttaag tacattttct
    29821 aatcttatga gtctattttt catagataat tgccctataa tagaagaatc tatgttggtg
    29881 ctatgtggtg acaataacag cattgtcagt gattaattaa tgctttataa atgattaatt
    29941 agtgttttat gttatttttg gacttaccct ctatacatag attaatttat ttaactttag
    30001 atagagagag aatgtcaagt acatacacag acattagagt ttaagaaagt gtttatggag
    30061 caagaacttt taaaagaggt agtgacttag tttaatttca agaaatgata aatacaaaag
    30121 aggaaaaaga gaggtgttga aatgatagaa aaagtagaag aaaaatctaa gattattaaa
    30181 acatcaaaag aatataagcg aactataatt gaagaaaaag ataaagaatg gaccaaaaaa
    30241 ttaaatgata tacttagtag aagtagtttt tctaatgatg aattagataa aatcaaggcg
    30301 ttaattccaa aagagatttt gactaatgaa aatgttggag aagttgtgac tgaagatgga
    30361 tatgctaagc ccgagtatga tgaggtattt aaatcattgt tcactttaaa taatgaatat
    30421 gatttacttg taaattttat aaatgatatt ctaaaagatg ctccatatgc taacaggaat
    30481 atcaaaccgt tcactcaaat aaagagaata attagagttg aaactgatcc aacaattaat
    30541 tacattggag agaaaagacc taaacttgat attttggctg aagatgaaga aaataatcat
    30601 ataaatattg agatgcaaag agcttttgag aacgattatt tggaaagagc tgaatattat
    30661 cttagtcgtg tacatggtag aaagttagaa gaaggtaaag agtataaaga aattggaaaa
    30721 actattggaa tacatatact aaatcatgta aaatataatc acatagaaga ttatgtaaat
    30781 tgcctgagat taacaatgga cgggcatcca gatatttatt ctagtaaaac tgcattatat
    30841 tttatagagc ttccaaaaat tcataaatct gactatatag taaatagaat tatgctatgg
    30901 ggaaaactta ttagtaatcc ttctaactta gatgtaagag ttatagctaa gaatgattct
    30961 attattaaaa aagcacttga tagattaaaa gaattaggta gcaatgaaga atatttatta
    31021 aatctgaaaa gaggtgctta tatcatgaat aaaagtagaa attttaaaga agagattgaa
    31081 agagagacag caattagagt tgcaaaagaa aaagattata aaataattat aatgttgaaa
    31141 aaaaatggtt ttaaattaga tgacatatta aataaattta atgattttga cttaagtgaa
    31201 gatgagataa aagaagtata tgaaaataat tgatgataaa taaaaagtga acttatataa
    31261 gtttgctgaa tcggagtctg gaaatattta tgtgtatttt ttaatgaatg aaaatttatt
    31321 ttaaaaacaa taatttagaa gtacagttaa ttatattttt atgatactgt acttctagtt
    31381 ttttctattg tagatttatt atttactaat aaatctacaa tagaaatgct tttgttgtaa
    31441 gaatcatttt atctaatgat tgttttttat aatagttaaa tttccatttg tagtgtttaa
    31501 atataaatct ataacagcat ttctataaat gttataaatc actataatga ttcaactcaa
    31561 agttactgct taaagaagca gagattttat caaggtatat catggaaaaa aatattttat
    31621 tgtaatattt ataagatttt attatggaaa taatgtaaaa acttta
//
LOCUS       DAMJWU010000038        29499 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMJWU010000038 DAMJWU010000000
VERSION     DAMJWU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.038"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(37..2703)
                     /locus_tag="PC460_001909"
     CDS             complement(37..2703)
                     /locus_tag="PC460_001909"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF2600795.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(2779..3041)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3337..4164)
                     /locus_tag="PC460_001910"
     CDS             complement(3337..4164)
                     /locus_tag="PC460_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2600796.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(4212..5648)
                     /locus_tag="PC460_001911"
     CDS             complement(4212..5648)
                     /locus_tag="PC460_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2600797.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(5735..6604)
                     /locus_tag="PC460_001912"
     CDS             complement(5735..6604)
                     /locus_tag="PC460_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2600798.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(6762..7415)
                     /gene="phoU"
                     /locus_tag="PC460_001913"
     CDS             complement(6762..7415)
                     /gene="phoU"
                     /locus_tag="PC460_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2600799.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(7443..8207)
                     /gene="pstB"
                     /locus_tag="PC460_001914"
     CDS             complement(7443..8207)
                     /gene="pstB"
                     /locus_tag="PC460_001914"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2600800.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(8226..9050)
                     /gene="pstA"
                     /locus_tag="PC460_001915"
     CDS             complement(8226..9050)
                     /gene="pstA"
                     /locus_tag="PC460_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2600801.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(9052..9969)
                     /gene="pstC"
                     /locus_tag="PC460_001916"
     CDS             complement(9052..9969)
                     /gene="pstC"
                     /locus_tag="PC460_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2600802.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQGIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(10350..10796)
                     /locus_tag="PC460_001917"
     CDS             complement(10350..10796)
                     /locus_tag="PC460_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600803.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVP"
     gene            complement(11312..11992)
                     /locus_tag="PC460_001918"
     CDS             complement(11312..11992)
                     /locus_tag="PC460_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600804.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(12042..14057)
                     /locus_tag="PC460_001919"
     CDS             complement(12042..14057)
                     /locus_tag="PC460_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2600805.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(14129..14806)
                     /locus_tag="PC460_001920"
     CDS             complement(14129..14806)
                     /locus_tag="PC460_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600806.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(15386..15469)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(15702..16544)
                     /locus_tag="PC460_001921"
     CDS             complement(15702..16544)
                     /locus_tag="PC460_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2600807.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(16796..18580)
                     /gene="pepF"
                     /locus_tag="PC460_001922"
     CDS             complement(16796..18580)
                     /gene="pepF"
                     /locus_tag="PC460_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2600808.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLVHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(18646..19311)
                     /locus_tag="PC460_001923"
     CDS             complement(18646..19311)
                     /locus_tag="PC460_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2600809.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(19462..19623)
                     /locus_tag="PC460_001924"
     CDS             complement(19462..19623)
                     /locus_tag="PC460_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF2600810.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(19701..20210)
                     /locus_tag="PC460_001925"
     CDS             complement(19701..20210)
                     /locus_tag="PC460_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF2600811.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            20416..20634
                     /locus_tag="PC460_001926"
     CDS             20416..20634
                     /locus_tag="PC460_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2600812.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            20652..22598
                     /gene="feoB"
                     /locus_tag="PC460_001927"
     CDS             20652..22598
                     /gene="feoB"
                     /locus_tag="PC460_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2600813.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(22777..23832)
                     /locus_tag="PC460_001928"
     CDS             complement(22777..23832)
                     /locus_tag="PC460_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2600814.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(23880..24680)
                     /locus_tag="PC460_001929"
     CDS             complement(23880..24680)
                     /locus_tag="PC460_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2600815.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(24673..25464)
                     /locus_tag="PC460_001930"
     CDS             complement(24673..25464)
                     /locus_tag="PC460_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2600816.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(25504..25926)
                     /locus_tag="PC460_001931"
     CDS             complement(25504..25926)
                     /locus_tag="PC460_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF2600817.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(25942..26421)
                     /locus_tag="PC460_001932"
     CDS             complement(25942..26421)
                     /locus_tag="PC460_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2600818.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(26553..29309)
                     /locus_tag="PC460_001933"
     CDS             complement(26553..29309)
                     /locus_tag="PC460_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2600819.1"
                     /translation="MRDEILSFVNECVLFNSFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGIAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         9457 a         5233 c         3213 g        11596 t
ORIGIN      
        1 tatatacttt ttgtatgttt ttactaattc ttatatttat tttattttag attctaaatt
       61 tgttaatctt tcctcaacag atttaatcat ctcttcaaat ttttcttttt ttgctttttc
      121 ttcttcaatt aaactttcag gagcttttgc taagaaacct tgatttgcaa gctttccatt
      181 aactctcttt atttcgcctt ctaacttttt cttttcttta gataatctct caatttcttt
      241 ttcaaaatca actaattcat taagaggtat aaatatttca acaccatcaa taactactcc
      301 aactgcatct tctggaacat tagatttatc ttttgctatc tctacatttg aagcagatgc
      361 taaagttata aaatagtctt taccaagctc aatagcttct attttttctt cacttgggat
      421 tattatcaat ttagctttct tagaaggtgg tacattcatt tctgctctca catttcttat
      481 acttcttata ccttccatca ataagttcat catatcttct tcttcagcca tgttatcttc
      541 ttcattatat tttggccatt cagatactat tatacatcct tcaacagtag gaagatgagt
      601 atatatttcc tctgttataa atggcatata tggatgtaat aattttaata tcttttcaag
      661 aacataagtt aatgtatata atgctgcaga tttagcattt gcatcttccc catataatct
      721 aggtttaacc atttcaatat accagtcaca gtattctgac caagtaaaat cgtatatctt
      781 ttgaagtgct attcctaaat caaacttatc catgttatta gttgcttctt ttacaatatt
      841 atttgctctt gatattatcc atttatcagc tagagttaag tcctctttaa cactttctct
      901 agtcatgttt ttaattatat cttcatcaat attcataaat acaaatcttg atgcattcca
      961 caacttattt gcaaagtttc tagcaaattc tactctttcc atatagaatc tcatatcatt
     1021 tcctggggaa tttccagttg ttaatgtaaa tcttagggca tcagctccat attgctcgat
     1081 tatctccaat gggtcgatac catttcctaa agatttactc atttttctac cttgagaatc
     1141 tcttactaat ccatgaacca aaacatggtc aaatggcttt tcattcatac agaacatacc
     1201 tgcaaatgcc attctaacta cccagaagaa tattatatcg tatcctgtaa caagtacact
     1261 tgttggataa taataatcta aagcttcagt cttatttggc catcctagag tagagaaagg
     1321 ccataatgca gaactgaacc aagtatctaa ggcatcttca tcttgtttaa aatgagtgct
     1381 tccacattta ggacacttat ctggcatttt tctagcaact actatttctc cacactcttg
     1441 acaataataa gctggtattt gatgacccca ccataattgt cttgatatac accaatctct
     1501 tatattttct agccattgta aataagtttt atcaaattta tctggaacaa attctaattc
     1561 tcctttttta agaatatcta tagctggttt tgcaagttct tccatcttaa caaaccattg
     1621 ctctgataat ctaggctcaa ctactgtatg gcatctataa caagttccta cattatgatt
     1681 atgctctttt atcttaatta ggtaaccttg ttcttttaag tctgctacga tagcttttct
     1741 acactcaaat ctatccatac cttcatattt tccacaaact tcattcatag ttccatcttc
     1801 attcatagta tttaactgct caagattatg tcttaatcct acttcaaaat cattagggtc
     1861 atgtgctgga gtcatcttaa ctgcaccagt tccaaactct aaatcaacat aactatctgc
     1921 aactattgga agttctcttc ctactaatgg aagtattgct gtttttccta ttaaatgttt
     1981 atatctatca tcttctggat ttactgctat accagtatct cctatcattg tctcaggtct
     2041 agttgtagct atttctaaaa agtcttcact atcttttagt gggtacttta tatgataaaa
     2101 gtttccgtcg tgttcttcat gttcaacttc tgcatctgat aaagtagttt tgcaatctgg
     2161 gcaccagttt attattctat ttcctctgta tatgtgacct ttttcatata ggcttacaaa
     2221 aaattctata actgcttcat tacacccttc atccatagta aatctttctt tatcccaatc
     2281 acaagaatca cctagttgtt taagctgatt agatattttt cctccaaatt catctttcca
     2341 ttcccaggct ctctttaaaa attcttctct acctatttca tactttgtct tgccttcttg
     2401 tttttttatt ctttcaacaa ctttaacttc tgttgctata gaagcatggt cagttcctgg
     2461 aagccaaaaa gcttcatatc catccatacg tttccatctg attaatatat cttgaagtgt
     2521 atggtcaaga gcgtgtccca tatgcaattg ccctgttata tttggtggtg gcatcattat
     2581 tgtaaatggt tttttatctg gatttggttt agatttaaaa tacccttcat ccatccattt
     2641 tttatatagc ctagcctcaa aatcttttgg gttataagtt tttgataaat tcgtattttc
     2701 cattgtcctg cctcctgtat tttataaata aattttttaa atatctttag aaataaaaaa
     2761 gagctcttca tcctctttaa aaaggacgaa aagctcgcgt taccacctta attccaaagc
     2821 ttaaaataca ttaacatata attaaattat tggttaatgt tattttatca actttgactc
     2881 tcaaagaatt taacggttct aaccgtctaa acttacttat agttcagttt agaaactcca
     2941 aagctacatt cagtaaatct tatacttaga aaatcttaca gccagtgaat tttcctctct
     3001 tttagtagta tttacttact cctcttcttc tcagtcttta atattacata ttattatata
     3061 caaatattat tctctttgtc aattattttt attttatttt ttctgaattt tcttaattat
     3121 gtctattctc ttttatatag ttgttatatt ttaaaatatg tttttacagt tattctgtct
     3181 tataatgtca ataattgcaa gattatttta ttttaatttt aatgctttct cattaatttt
     3241 atattatcct tacagtatac ctcttattcg tattttttat attatatgtt ttattttata
     3301 aataatttac tttatctttt actatattta aatttattat aagttttctt gccagaaatt
     3361 tttaggtgtt atattttctg taattggcaa tattgtatat cctctctctt ttaagattct
     3421 tataatatta tttaaagcat ttaaactttg ctctttctca tgaattaata ctactaattc
     3481 atctcttttt cttccataat ataatatatt acttactatc tgacttgtag aagacttcca
     3541 atcctgtgta tccaaattcc agtcccaaat taaataatca tttgccataa gtttattata
     3601 tgaaccttct ggcatatatg gtttacttcc gtatggtaat cttattaatt ttgaactctg
     3661 accagttatt tcttgtagag tattattgca tgtattaaat tccccaactg ttacttctgg
     3721 gcttttatat aacttatgta tatcatgact tacactatga aaacctattg agttttcttc
     3781 ttccaagact ctccttactt cttccttgtg gtttttcata ttctgattaa gcatgaaaaa
     3841 tgtcgctttg acatcatttt cttttaaaat atcaagtagt gcatttgtat attttgatgg
     3901 accatcatct attgttatgt atgctacttt ttcagaagta gcccctggac cttttgtttc
     3961 ttcttgagat gctttgtata tgatttctcc taaagtatta ctttgagaaa gcagttccag
     4021 tgtattttca ccttttgaac ttatattttc aactggaaca ttattaattt tttcctccaa
     4081 taatgttcca gttaaaatta aaacagctat aagaaatatc cttataaata catgaaaaac
     4141 agccttatta tctttaattt tcatccccca tatctccctt ttataaaagc ataagtttaa
     4201 gacttaaaaa gttatttttt atatttattc tctttcttat tactttttat atctctacta
     4261 aatatttttt taaccttttc ttcttctttt tctcctaaag attctactct tttgattccc
     4321 aaagaagtta aaaattgtaa tacatttaaa ttatcaaatg gagtttccat attcgtactt
     4381 tcactatatt catcaacatt cttagtacct tcttccacac ttaaaatacg cttcggacct
     4441 cctacacagc ctcctacaca ccccattcct tctataaatg tagcttctat ttctttattt
     4501 agaactttct ttagaccgtc catgcactct tttgttcctt caaatgcctt ttcttcaaat
     4561 tttatatttt tgtcaattct tctaactgat aacttaactg ctttacttac acctcctgtt
     4621 ctagcataaa ctctacctga aagggacgcc tctactctat tactctcatc cattattgct
     4681 ggatttaact ctaatgcatc aaatatttct tgcaactctt taaatgtaag cacaaaatca
     4741 actgcatctt ttatatcttc taacattgct tctttctttt ttgccataca aggacctatg
     4801 aatacaactt tagcatctgg atttaaaatt tttacagctc tcccacaagc aatcatagga
     4861 gaaacggatg gtgatacatt ttccaatatc tgaggaaaac tattttgtat caaactaacc
     4921 caaataggac agcaacaact agttataaag catccttctt cgtccttttt catatgctca
     4981 taataatcat atgcttcttt tgcagtaagc atatctgctg ccaaagcaac ttctatcata
     5041 tcttcaaatc ctatcttttt taatgcagtt cttaatttac cagggcttac atctttccca
     5101 aactgtccta caaaagctgg tgcaactatt gcatatacag ggctctttat atcttttaac
     5161 aattttatca ttggtataaa ttctatttta tctgaaattg cacccaactt acatcttgat
     5221 acacaatcac cacaagcact acatacatct tcctcattac aattaatatg cttacttctt
     5281 atcttacatg actcatcttc cttattattt tcatcacaat atttgcaagc atctgatatt
     5341 acagatatta ttggtctttt gacttcatct aaatttacag cttcatgcag tgactctttt
     5401 aaatttttat ctgtatcttc tgtaacatcc aatcccatag ttaccctaat taaatttacc
     5461 atagtatctt tatcaaattt tccactaaaa taattattta aattatcctt taaatcttca
     5521 tttaaaattt tattaggtaa gttttcaata tcctctatat catcattcca cactgctttt
     5581 acaagttcgc caaaaagctt cattttatat atttctagta ttttagaatt gtcatttcta
     5641 gtattcataa aaaatagtac ctccataaat taaacttata tatgtttaat atttacaaag
     5701 atactatttt ttatttatta tttaactact attattaata tgatttattt agctttctca
     5761 acaactctat ttcaatcatt attttcatta gatgatagtt gaatacaggg cttttttctt
     5821 catctatatt ccagtctagc ttatccacat tatatgtttt cttaaggtat ttatatgtat
     5881 gctctttcaa ataaagttta ttttcaagta actctataga ctgcatatga aaatgtattt
     5941 ctctacatat ccttaaggca ttctcaattt tttctatatt agtttcactc aaactataat
     6001 ccaactcatc aataagtttt gaatatattt cttctaattt ttctacttct tcttcaaatt
     6061 tctctacatt taaattttct tttctaagaa ttttatcttc aataagcaac attgaaagtt
     6121 cttctatctt tttaaactca tctataacac tatttagata atttggtcta gcaacataat
     6181 aattaacaag tactccaaac aaaacaccca cagatgtatc taacacccta tgtaaagaat
     6241 aatacacagg gtcactattt cctacaccta gatgtatagc cagaaaggtt acacaggcaa
     6301 ctactactga ctttatttta aacagattgc ataaatatat tgagcacatt actcctagac
     6361 cacataaaaa tgggtctcca gctttaatga ttgcaaatat gaatcctatt ataccaccta
     6421 aaatagtacc ttccactcta ttgattcccg tctttaaaga accttttact gtatcttgaa
     6481 cagaaattat acatgctacc agagaataaa acatattctc tactaaatag tcaccaagaa
     6541 tcatacataa agtgacacta attcctgttt ttatagtcct catacccatc ttttgaagtt
     6601 tcaaagttcc acctcctaaa aagcacctta tataaaatac gcatcctcat atttaatatg
     6661 ttcctagtat atcacaattt tttaaattta cccattaatc atattttttt atcaattctt
     6721 tcttaattta gacaataaaa aaactgcttt aaagcagttt tttatcctat ctcaaccatt
     6781 ttaccctcaa ttgcaaaaat tatcttctca catacatttg ttatatggtc tccaattctt
     6841 tcaaggtatc ttcctataag tagaagtttt accccttggt ctatggtatt tgagttttca
     6901 tgcattattt ttaaacaatc aatttgtact tgttcataca atccatctac aatatcatct
     6961 ttagttccaa catcataagc tatacttgca tcaaataaga ttaaagcttc tctagtctga
     7021 gacatcatag acttacattc ttttgccatc ttaggaatat ctattagctc ttttatataa
     7081 gcctctccac ctatcttcaa catttctcta gctatatttg ctgcataatc tcctattctt
     7141 tccagctcta tagatatatt acctaatgaa taaataaatc ttaagtccct tgccattggt
     7201 tgctttaata ctattagctc tatactctta tctcttattt cttctcttaa tttatttatt
     7261 tcttcatcat cttctactac ttttcttgca ccctctaaat ccttctctat catacaagtc
     7321 acagactttt ctattgcttc ttcacatttt tccatcataa gcactgtttg ttcttttaaa
     7381 gtatcaatac ttatatctaa actattattg accattgtct aactccttta tagttatata
     7441 ttttaaccaa atctacctgt tatatagtct tctgttcttt tatctacagg agtggtaaac
     7501 atagtttttg tatcgctaaa ttctataacc tctccattta agaaaaaagc tgtttcatct
     7561 gatatacgag cagcttgttg catattatgt gtaactatga ctattgtgta gtcttttttt
     7621 aaatcctcta taagttcctc tacttttaat gttgaaattg ggtctaaagc agatgtaggt
     7681 tcatccataa gaataacttc tggtctcata gcaattgctc ttgctataca tatacgctgt
     7741 tgttgaccac cagaaagtcc tagtgcagat gatttaagcc tgtctttaac ctcatcccaa
     7801 atagctgcac cttttaagct ttcttctaca attttatcta attgcttctt atctcttagt
     7861 ccatgtgtac gaggaccata tgccacatta tcatatatac tcataggaaa tggatttggc
     7921 ttttgaaata ccattcctac tttagtacga agatttataa catcatctga ggtatatatg
     7981 tcctctccat caacagaaat gtttccttta attgttacat cttcgattaa gtcattcatt
     8041 ctatttaacg ttcttataaa agttgatttt ccgcatccag atggacctat taaagcagta
     8101 accttattct cttttatatc catatttatt ttctttaatg cttgcttatc accataaaat
     8161 aaatccaaat cttttacact catttttatc ttatcgatta attccattat tcacacctct
     8221 taaatttaat aattagcttt atttaatttc ttagcaacaa atcttgctag catgtttagt
     8281 gctaatacaa ttactattaa aacaattcct atactggctg ctgttgctat atctccactc
     8341 tctttagtca gtaagtatga gtgtacagtt aatgttcttg cactatcaaa tattccacct
     8401 ggcatcttgg ctacagtacc tgctgtaagt aatattgcag ctgattctcc aattatacga
     8461 ccaactgata agattactcc agataaaatt cctggtattg cacttggaag tactacctta
     8521 tataaagttt gaaattttgt agctcctaat gccaatgatg attctctata agcttgagga
     8581 actgttttta aagcttcttc tgttgtacga attattactg gtaatattat tattgcaact
     8641 gtaagtgaac ctgcaactat ggaatatccc atttttaatg taactacaaa gaaaattcct
     8701 ccaaataatc catatactat agatggtatc cctgataaac tttcagttgc aaatcttata
     8761 gctgtaactg cttttccttt tttagcatat tcttgtaaat atatagcagc caatattcct
     8821 ataggagttg ctacaactat agaaactact actgtatata atgttgtaat tatcattgga
     8881 agtataccac catcacctga tgctgaataa ttgctaaata agaaattaaa attcatgttg
     8941 ccaattccct ttataaatat aaatcccact ataactacta atgatactat tgtaaatagt
     9001 gctgatagat atactaatga cttcaataga ttctctttga actttctcat cttaatttac
     9061 cgccttattt gaaagtttat ttaaaactaa gtttaaaatt aatataaatg taaacaatac
     9121 tactccagtt gcaaatagca tttcttgatg tgtaccaaat gcgtatccca tttcaagtgc
     9181 tatattagtt gtaagtggtc ttacactatc tgttattgca gttggcataa ctggtgagtt
     9241 gcctgcaaca agtattactg ccattgtttc acctaaagct cttcctactc ctaacactac
     9301 tgcggctaat actccagatt ttgcagcagg taatacaact ttgaatattg tttctatttt
     9361 tgatgcacca agtgctaatg aaccttcttt gtatgctttt ggtactgctc ttattgctgt
     9421 ttctgataca gatattattg ttggtaacat cattactgct aatactatta ctattgctaa
     9481 caaactttga ccttttggaa gattaaatat accttgtata gttggaacta tcactgcaag
     9541 accaaataca ccatataata ctgaaggtat acctgctaaa agttcaactg ctggtgacat
     9601 tatttttgct aatcttttag gtgctacttc tgctataaat acagaggtta atataccaac
     9661 aggtactcct ataacaagtg ctccaagtgt tgcaaatatt gatgctacta tcatagatga
     9721 tattccaaat ttattggcag aaggaaccca atctgcacca gttaaaaagt ctataaatga
     9781 atatccttca aatataaatg gtcttaatcc tttataaaat acgaatccta ttatcaaaag
     9841 taaacttgca actgctacta aagcactaag tagaaaaata ttttttgcta ctttttcaat
     9901 tacgtatttt gtcttattgc cattattgtc atttttttct aaacctacta atctagattt
     9961 tactatcata ataccctccc attaaaatat acttatcgat tttcgttctg tattcaaaat
    10021 ttaatttttt ataattcgtc ttcatcaaat ttcctatact tttattctac tttttttaag
    10081 ttaaatctat attaggatta agttaagcaa gtgtaaaatt atatttactt tttaacatta
    10141 attttacttt taaaactata tttgacattt actttacaat atactgttgt ttctctatat
    10201 taaaatagca gatatagata ataatacagt ttatgtataa tttattacct atatctgcta
    10261 ttaagatttg ttaactaaat ttaactttta tactacatat ataattattg tatcctctag
    10321 tttatatcac tattatttcc atcaaattgt tacggtactt gatgtttgca ataggtacga
    10381 atagccaaaa atataaaaat aggtatttct atcactgaga aaaacagtat aatgtgtggt
    10441 gtccaaaaac tcatacctcc taagtgtaag aaattaaatg atgtcatttg atataataaa
    10501 ctaatcttaa aaccaattcc ccctggtggt aataggtatc caatccaact aacacctatt
    10561 cccataggaa ttaagcaaat agaaaaagca atcagcatag aagtaagaga atccttacat
    10621 tttgcagata aaaataatgt taaactaacc attgcaagta ttgaaattag accaccaata
    10681 gctataagaa tctgtgcttg tagtaaattt atatttacta gactaatgac agaaaacaac
    10741 atttgaaatg aggttttcaa acattcagta ccaaaagcta aatcagaaat tgccaaatgg
    10801 atagaaatac atataacaaa cattattata aatatagaaa atagagctaa tattttagta
    10861 aacgctagac gaatatgtcc atgtttcgta caacgaagga tgctatctga ttgtgtctga
    10921 tactcaccag aaaaagtagg tgcagcgatt gcaacacata taattaataa tataaaaata
    10981 taaaatatta tataatcgaa agcatcttct ggatttatta agaaattctt aatcttatta
    11041 tttaactatt ttattattta atatatatta aataataaaa taatatatag atttagatat
    11101 acaatttatc catcttttca aatataatct ataaaataat agttccatta cgcatttttt
    11161 tatataataa aactgactgt aatttaactg gcaaagtcta gaagaatagg atttatcaat
    11221 taaattacag tcagctattt tataataatt tagtttagat atcgttagta tttcacattt
    11281 ataacaatta ttaatgatat taatatattt attattcctt attaaaataa tatcctgctc
    11341 ctcttattgt tataattttc tgaggatttt tataatcttt ctctattttc tttcttaatc
    11401 cattcactgc aactaccaca ctatagttat cctctaagta ttcattcatc catacatgtt
    11461 catatatctc tttagtagtc aaagtctttt caggattctc tataaaaaag tttaacaact
    11521 tattttctgt tggagtcaat tttattattt tatcttctaa ctttattact ccactagttc
    11581 tattatattc ctctaatttt gtattatttt cattattatt tacatatgag ttcattcttc
    11641 ttaaaatagc ttttactttt gcgtataata tttctaaatt tagaggcttt attaagtaat
    11701 cgtctccacc agaatttaaa gcatttaaaa tgctttggtc ttcatttata taagtcatat
    11761 aaacaattgg acaagtagtt acatttctaa tttttttaca aagagtccat ccatcaccat
    11821 cacttagaat tatttctaaa aatattaaat catatttatt gctaaatatt tttccaatag
    11881 cttcttcaca attatatgct aaatctatac ttattccttt ttcctccaga aatcctttta
    11941 gcttctggca attatccttg tctccatcta ttatcaatat cttagtttgc attataatca
    12001 ctcccaaata tcattattct tttatattaa ataagctatt attataaaaa acatagactt
    12061 aattttattg taaaaacagt tccttttcct agttctgaat ctacaaatat ttctccacca
    12121 tgctctctta taatggattt gacaatatat agccctaacc cttgcccttt tggctcatta
    12181 ttgtctcttt ctgtaaagaa tctgtcaaat acctttggta tattttctgg aggtatacct
    12241 tttccattat cagaaatttt aataattgca aatccatctt ctttatccat tgataaaatt
    12301 atctttccat caaatggagt aaaggtcaaa gcattataaa ttaaattctc aaatgctctt
    12361 gtaagcatct ctttatttat ctttgcatat atttttttct ccaatacata agtttcaaag
    12421 tttacaccac atgcttgtgt atctggcaaa ttattttcat atatctcttt aataaaatta
    12481 ttcagacata ttttttcact aataccaatg cttttatctt gtgatgtaaa ttcttgcaaa
    12541 ctacgtactc tattttgtac ttcatttgac ttttgattta tagcataaag ataggttctt
    12601 gtctcttcat ctacatgaat attaccaacc tttatcaaat ctataaatgc ttgtatagcc
    12661 gaaacaggtg cctttaaatc atgtgctaca tctgccaaga atcttttacg ttcatttaac
    12721 aatgttgtaa gttcattagt tctcacttca acttcatgtt caagattcct tgtaagtttt
    12781 tcattctctt ttattattat tctattcctg tttatcatca taacagaaaa taaaataaca
    12841 attacaaacc cacaatattc tgtttgccac cctgtaaata taggctcaaa tctattactc
    12901 gtaactgcat caaataatat gctaattcca aaaacagtgt ttactgaaac taaaatataa
    12961 gaacttatat gctcagagca tgtcttttta tatattgaaa ccagcgaaga cattaatata
    13021 tatataaata caatatattt atatatgtct attaacttcc catatatgtt gacaaagtac
    13081 atatcatatg gaagaagaat aattggtatc actatagata tgatactcat tgtaattcct
    13141 aatggtaaaa ttaaaaatct atacacttta ttttcttcta ttttggatat acttccattt
    13201 atacaaataa tacataaaac taccataaaa tatgacaaat cttcaaccgc atatgtaaag
    13261 cttaccaaag gcagtcctaa caaatgtaca aagggatagg atacatggat ggcaaagaaa
    13321 acacacatac atccaaaata taagtaaatt ttacttttgt ttgaaagaaa ccatacagac
    13381 aatgaaaata tagctattgc aaaagttgtg aagcaaatta tactataaaa aagtaatcga
    13441 taaaacatca tattacttat gtcctttgtt tctcctaatg caggcggata atacaaacca
    13501 ctataataat gtgaaaaatt cgatacattg attataattt ctgtattttt ctttgcaata
    13561 aaacttacta ctgaattttt aattttgggt ctatattttt ctgttcctac atctcctaat
    13621 ttagaaacca tttctccatt tatccatact tcacttgcgg aaaaaatctc aggaagctct
    13681 aaacttagta gcttatcctt gccttcattt tttattctca tacgataagt agcattccca
    13741 tatggtgact gagtcccttt tctcatagaa aaatctggat attgacctat aaaaacatat
    13801 tctggtttta tatcttgctc tgtaaaatca ttcggtttaa atattttatg attataaaac
    13861 tcccagtcat ctatcaaaaa gacaagttta cctttagtga aagtatcatt atctatagtt
    13921 aaaattccat tacttgctct aatagactta tatgtatatt tattatcata tttatacaga
    13981 agatttaatc ctgttattgc cagcagagag actatcaata caatgagagt tccttttagt
    14041 atattatact ttttcattgt atcaaccaca tctctttcat agtagtatta aataaaacaa
    14101 gaactattta ttaattacag tatctcactt atggtacaaa cttatatcct tttccccata
    14161 cggtttgaat gtattgatgc tccctgctta aagaattaat tttttttctc atctgtgcaa
    14221 tatgaacctg cactgtatgt tgatctccaa tatcaggcat tttccaaact tggttatata
    14281 tctctatctt tgaaaatact tgttctttgt tctcagctag aaacaataat atttcaaatt
    14341 ccatagtagt taaatctata ctttctccat caaaagttac ttttcttgaa tttacattta
    14401 ttactaacgc tgaaaacttt aacacattag gaactgatgt aatatttttg tactgcctca
    14461 tacgagcata tattctaagt tcaagttctt ttaaactata aggttttgtt atataatcat
    14521 cccccccagc taaaaatccc cgaacttttt cttcttcatg ctcataatta gataaaaata
    14581 ttataggtat gttaaccttt tcttttatat cattgcacat tttatatcca tctatatctg
    14641 gcaatgaaat atccaaaatt atacagtcta aaactatact ctctactata tccatagcat
    14701 ctttagcagt ctctgatgta tatacattaa aacctatatt agaaaaataa atttgattta
    14761 tgtttaaaat atcaacatca tcatctacaa ccagtaaatt atacattaca ccactccatt
    14821 caaaggtatt ttaattataa ttccaagttt tatagttatt acaaattata atgttttatt
    14881 ttcatatata actggtatta ttattttata atatctttac aaactagact ataatactta
    14941 agtatttttg aataattctt gaatattgta ttaaacatta agattcatta ttaaatacta
    15001 attaaagtct ccgattgttg gagaatggag cttaaagtca ttttcacgta taaatttatt
    15061 atttttctag aaacttgtct attggctagt ttgagtattg atttatatga tttatatggt
    15121 tataatataa aataaattgt attattttgg taaatatatt gttacaaaag gtttatattc
    15181 tgcatctcaa tgtttataaa aactttattt tcttattata atactattat tcttttaaaa
    15241 aaatttccct tttccaacaa atggctactt taaaagccaa aattttacct atcaataaaa
    15301 atcttaacga ctaatactct tattttcaaa ttttgcaaca tttttgttaa ttttaaaaca
    15361 aaaaaagact atgcaatata aaaattatat tcatagccgg ttgcaccact aactccatat
    15421 atctctagat gcccaataat aagatatact tctcagtttc taaaatattt ttattaaaaa
    15481 tcttttatat attttcataa atttcttttt tattctaaca ttgtaatatt attttgtcaa
    15541 tatttccata ttcttagaga taagatacta aaatacctat tttatctaaa gatatttata
    15601 tttctacatt caataataga caataccctg caagttaaag catcattttt atgagcttat
    15661 ctcacagggt attatatgtt tactttatat tataactctt gttattgatt aactttaata
    15721 aatccttttt ctgaaacaag tgcttgtcct tcatcactta atatataatc tattaaagct
    15781 ttaccttttt ctgacaaacc atcttcttta ttaactaata taaatggtct tgatattgga
    15841 tatttattag ctattacatt tgcatcagtt gcttcaacat catttatttt taaactattt
    15901 actttctcat caacatatcc aaatgatata tatcctatag cattttcatt tccttcaact
    15961 gtagttttta tatttcctga accatcactt atttgtgcat ctttagttag ttcttctgaa
    16021 tcaaacccta caatttcttg gaatccatct ctagttcctg aaccttcttc tcttgatact
    16081 attactattg gtgcatcatt tcctcctact tctttccagt tagtaatctt tcctgtatat
    16141 atacctttaa cttggtccat tgtcagattt tttactgagt ttgcagtatt agttatcaat
    16201 gctataccat ctattgctat tttagtttcc tttaatccag cacttttttc ttcatctttt
    16261 aaatctcttg aactcattcc tatttcagat gttccttcta tagcattttt tatacctcca
    16321 gaagaaccaa tctgttgtac ttctacacta acaccttgat ttttttcttg aaacttttct
    16381 ccgatagttt ccataagagg acctattgaa gttgaacctg aaacagaaac tttgcttgaa
    16441 tctcctgaat cacctgaacc tcctgagtta gaactacatc ctactgctaa agctccaact
    16501 aacattgtac tagctaaaag aacgcctatt ttctttttaa acattttact tacccccgct
    16561 tatattttaa ttttttgttg tcatttttct atattttaat aatacagatt ctttgttaaa
    16621 tgaatattag agcagggtta agtaaatgta aatattgtta aatacatgta aaaacttaat
    16681 tttatatgat ttattcttaa ttagattaac tttttaaagt tactttaggc agatataaac
    16741 ataatacaac tcatattatc agaagtcttc attgaagcta tcaacctatc acatcctata
    16801 attctttttc tagtttatta actaattcag caaatacatt taaggcttcc tctatagatt
    16861 cttttttcgt catatctaca ccagcagatt ttaattgttc tagtgggtat tctgaccctc
    16921 cactttttaa gaagtttata tatctatcta ctgcagtact tccttctgaa agtatctgtt
    16981 gacttaaagc acttgcagca gaaaaccctg tggcatactt atatacatag aagtttgaat
    17041 aaaaatgagg tattctcgcc cattctattc ctatttgttt atctactatt gcagattttc
    17101 cgtaatactt ttcatttaat ttgtagtata tattagtaaa ttcatcagca gtaagtggtt
    17161 ctccactctc taccctttga tgtacaattt tttcaaattc agcaaacata gtttgtctat
    17221 gaactgtagt tctaaattgt tctaaataat aatttaataa gtatatagtt tcatccttag
    17281 acttagaatt ttctaataaa taatttataa gaagtaattc atttaatgta gatgcaactt
    17341 ctgcaacgaa tatcttatag tccgaatata aaaatggttg acttgttctt gaataatgac
    17401 tgtgaacaga gtgccctaat tcatgaacta aagtgaatag agaattaagg tcatttttat
    17461 agctcattaa tatataagga tgtgaatcat aacttcccca agaataagct cctccttttt
    17521 taccttcatt atcatataca tcaatccacc cttcatcaaa agcacttttt atgaccttca
    17581 agtattcttc tcctaaaggt ttaagtgcct ctaatatgat atcctgtgct ttatcatatg
    17641 gtatatccat gtcaaattta tctgtcaaag gaacatacaa atcatacatg tgtatttcat
    17701 tcaatcctaa aaatttcttt ttaagctcaa catatttatt tagattagga atacttttat
    17761 ctatagctga tataagatta ttgtaaacat ctaaacttac atcatctgaa aataaagacg
    17821 cttctattgc tgaattatac tttctagttt ttgcgtaaaa tatctctgat tttattccac
    17881 cataaagcgt tgaggcaaat gtatttttat atttctcata tgttgaaaat tcaccttcaa
    17941 aagcgtcttt tctaactcta ctattcttac tttttataaa taaagagtag tttgaatgag
    18001 aaagcttaac cttattcccc tcctcatctt ctatctcagg aaattccata tctgcatatg
    18061 ctaacatatc gtatacattt tctggaattg tcgataattc tgatgtagct gcaagtattt
    18121 cttcctctcg ttcactcaaa gtatgtggtt tatctctcaa tatatccttt atatattttg
    18181 tataaaagga tagcttctca tcttttaagt attcacttaa cttattctct tctatcgcca
    18241 gtatttctgg aactatataa gaagtagcca cacctaaatc agtagataac atttctgtct
    18301 tagtggctct tccttgatta tcattaattc tagtatcttc atgttgtttc atatgagtat
    18361 atacatataa attttgaaca atcctagatg cattttcaga gatatttaat actttatata
    18421 agttctcctt actctcagaa agcttccctt tatattcttt tacttcttgg ataagatttt
    18481 ttactttact tatgtctttt tcaatttcct ctttactgga gtacatctta tctatcttcc
    18541 atttgtactt ttcttctatt gtacttctat cagttcccat atcatacacc tctattttaa
    18601 tttgttttat attttattca ctttacaaaa tatattgtat atattttagt ccaaataatc
    18661 tacatttatt actccttcta tcatatttat ttctttcata acatcgtgta agtgtattct
    18721 cttagcataa gatacaaaaa aattaacctt ccagtattct tcattacgac attctatttc
    18781 catatttctt acgataagtt gatttttttc aaatttatca tataaaaaag gtatagagtc
    18841 caaattgctt aattttaata tcaatacatt cttttcttgg ctaaataaaa acgtattgat
    18901 tatcctaagc acaaataatg ctatcattac aaataatcca cctagaattg ccaattcata
    18961 ataaccatat ccaactgcta taccaataca tgcagttgcc cacatcccag cagctgtagt
    19021 aagccctttt accccacctt tattttttaa aattgctcct gcacctaaaa aaccaattcc
    19081 agataatact tgagcaggca ttcttgctgg gtcagcattt gtatacaaat tataatcaaa
    19141 gaataattga atcgaagtta ttgaaacaat acaagaacct attgcaatta gtatatgtgt
    19201 cctaaatcct gcaaactgtc ttattttttc tctctctaag cctattattc ctcctattat
    19261 tagtgctaat agtaacctta ttgctatctc cttacttcct atatcaatca taatataaaa
    19321 ccctcctgac ttaattacaa cttatgtcct acagataatt tacacatcag aaatactatt
    19381 ttatatctta atattattat taatatatgt aaaatatagc catctgttaa tttttgatac
    19441 aaaaaaaccc acaactgtgg gctatttgct taggtacatg tatttattta ttaatatatc
    19501 tattttctta cttgattcta ctaactcttc ttgaaatatt tctggatatt ctatgtatgt
    19561 atttatctct tccctaactg cttcaatctc attttttaac gcttctaatt cattgttcgc
    19621 cataataatc accactatta caaatttgta tatttttata tacattttat caaacaatat
    19681 tatattttgt caatattatg tcaataaaac ttactataaa aatgctttaa tttaggtatt
    19741 gtatacctgg atactaagcc tacaaacata ccagttacta atgatgccat taacataaat
    19801 ggtaagtacc ctatagcaag tatattttct atgattaaac ttgcaactat taattgacct
    19861 atattatgcc ctattgcacc tataatgctt atccctatta gtgataaatc ttttatttta
    19921 ctcgctaaat acataaataa aagactcaga taagctcctc ctatactaaa tagtagatat
    19981 gataaagggc ctgcaaatat tgatgacaaa attaccctga tagtaacaat tataacagta
    20041 tctttgacac ctaacaacat taatgaaatt aaagttataa tattgctcaa acccaatttt
    20101 gcccctggaa atataactat aagaggattt ggtatatatg tctctaatac atataaagct
    20161 aaactatacc ctatcataag acctaaaaat gtcatttttc tagttttcat taagttaact
    20221 cctccaaatt ttatctatca tcttacatta taatacaaaa tatataaaaa cacctaattt
    20281 agactaaaat atttttaatc tatttagaat aaatattagt tatttcaatg tgcaattacc
    20341 tctataaatc ttgacttttt gtatcatact tgttacaatt ttaatgataa tgaatttcat
    20401 taggaggtgt taataatgac tgtttacgat ttaaaaatag gagaaaaagg atttattaat
    20461 agtatagatg gagatattaa attatctaaa agattgcttg cacttggatg tataaatagt
    20521 actgaaatag aagttaaaaa ggttgcccca cttggagacc caattataat tagatttaga
    20581 ggctttgacc ttgcgattag aaaatctgat gctaaaaata tttctttaca ataatagagg
    20641 aggagctacc cgtgattaat gtagcattat taggaaaccc aaatgtcggt aaaactactg
    20701 tttttaatct acttacaggt tcaaatcaat atgtaggtaa ctggcctggt gttactatag
    20761 aaaaaaaaga aggatttctt ggaaaagaga taaaggttgt cgatttacct ggtatatatg
    20821 caatggatac tttctcaaat gaagaaaagg tttctaaatc atatttagaa aatgaagatg
    20881 ttgatgtaat agtaaatgta gttgatgctt ctaatttatc aagaaacctt tacttaacaa
    20941 ctcaacttat gcaatttaac aagcctatag ttatattgct taatatgtta gacattgcag
    21001 aatctaaagg agttaatata gatgccaaaa aattaagtga agaacttgga gtaattgttg
    21061 ttccaattat agcaaaaaag aaaaatggtg ttgataggat agaagaagag attaagggag
    21121 ctacgcaaaa agcttttata tatcagaaaa actttggaag tgaaacagaa acatataaaa
    21181 aattagaatc cattttatcg agatgtacaa aaactacata tacacaaaag aaatctatca
    21241 gtgataagat agacaatata gtcttgaatc ctatattagc atatccgata tttattggtg
    21301 tattgttcct cttatttaaa tttacctttg actgggtcgg aggaccacta caagagggat
    21361 ttgctagtct tatagaggct tatatttctg cacctgttag tgatatgtta tctaactcta
    21421 gcccatggtt taaatcactt atagttgatg gaataattgg aggtgttggt ggtactttac
    21481 cattcttccc tttaatattt acattattct ttggtatatc gttgtttgaa gatagtggat
    21541 acatgtcaag aacagcattt ttaatggata aagttatgag aaaggttggt ctatctggta
    21601 aagcttttat acctatagtt atgggtatgg gatgttcttc tccagctata atggcaacta
    21661 gaacattgga aagcgaaaaa gatagaagag ttacagctct tattgcacca cttatgactt
    21721 gtggagcaaa gctacctata tatgcactat ttgtagctat attctttcca cataatgctg
    21781 ctttagtaac tacatcactg tatttggtgg gtatagttat ggcaattgta gttgcattat
    21841 tcttaaataa aacagccttt aaaacagagg ctgaaccatt tatattagaa ttaccagaat
    21901 atagaatccc tactatagat gcacttatta aaaatacttg gaataaatca aaaggtttct
    21961 tgataagagt tgttactgtc atgtttgcaa tgtcagtcgt aatttgggga ctttcttcat
    22021 tcaatgtttt tggatataca gaagatataa atgtaagctt cttggctact ctaggtcata
    22081 ttatatctcc tatattcaaa cctcttggtt ttgatgattg gagaacttca gttgctattt
    22141 taagtggtct tggagcaaaa gaaatcgtag taaactcttt aaatatacta tatggtaatc
    22201 taactgttgt cttaccaaca atatttaatg gagttactgc atatacattc ttgatattta
    22261 ctgctttata tactccatgt atagctgcac ttgcaactat gaaaaaagaa tatggcaata
    22321 aaatgatgtt tacatcattt gcatatcaat ttatactagc atggataatg gcttttatag
    22381 ttaaaagtat aggtggagta ctatttatgg gtaattcaat aatggaagct ttgataggtg
    22441 gaataattat agtccttgca ctctttattc tgtttaataa attcaagtct acaaaaaacg
    22501 gaaatgtctg tagtggatgt tctatttgtt ctggatgtcc aagtgctaat tcttgctcaa
    22561 gtaaggaaga cagcaataaa aaaaataatg cacactaata tataaatatt ttagtttaaa
    22621 ataagataaa agtgggtgtc tcaaaattaa ctttttagtt catgaggcac tcttttttat
    22681 ataaaatcaa atatattttc atcatttcaa cagtaaaaaa gagcttatct agtaaaaaag
    22741 aggcttatct ctattttgag acaacctctt tttttgttac ttattcagtt tcttgctacc
    22801 taacttcatg tggaacctag tatcttttgg aacacttggg tctacacctc ttaatgtagg
    22861 tatttcccca catattagtt gtataggtac tataaaatta aatgtttgat agtaaggatt
    22921 atctgtgata tcaatcttta aatttttatc attttctgta acatctctac ctatagcaaa
    22981 tacattttcc gtccaaccag aaagtacatc aatcatctta gttactctag gttcttttcc
    23041 tgtatctagt ataaatatag tagaatcact atttatagca ttatatatac catgtataaa
    23101 ctcttcaaac tcatacccag ttacaggtat tctcattgtt tccaataatt ttaatgctgc
    23161 ttctaatgta tctccatata tatcagagtg acctattatt cttatttctt tagaattaac
    23221 taacttttct ttgtttcttt caatccattg tttagagagc ttatacactg cttcaaatct
    23281 attaattgcg tctaatatct tatttatttc ctcattgtac ttttcactag atattatacc
    23341 tttttctcta gctatttgaa gacctaataa cattaagttt aactttgtac aataatatcc
    23401 ttttgtttta gctccagatt tttcttcacc acaatttact gttaatatat aatcagaaat
    23461 ctcatctatt aaagcattct tacaacctgc catagatgct attttacagc ccttatcttc
    23521 agctaacttc atagcgttgt atgtagaata actactccca ccttgagata cacctactac
    23581 tagggtattt tcattatcaa acttgaatgt atcttcagtt atcataaatg gatacatttt
    23641 cacaacatct atatctaata aattttgtaa atatggttgc acctgcactc ctgagtgata
    23701 gctagtacct gaacctgtta ttataatctt ttttagattt gttctcttaa cttcattaaa
    23761 taagctatct gcattagata taatttctct cattctaact ggtgtttcta acatataatc
    23821 ttgtattgtc attttataca ccttccttat ctatatattc aataaattat aattaatttc
    23881 tataacaaac ctatagcatg taaagcgatt cctgttacta tagtgcctat agttaatttt
    23941 gttacactta cctttctctt taacatgtag tatattacaa atgcaaaggt taaaggtaac
    24001 atattaggta ttattttatc tagtatatct tgtaaaatta cttctgcacc atttaagttg
    24061 aatttcagag gagtagttat actcaacata gttgcaacca ttcctcctac aacgaataaa
    24121 ccaacagttg tagccataga cattattttc tccataagtc cctctctttg tattctttca
    24181 agagaattta cccctacttg ataaccatac ttaagtccaa atattctaag ggcaaatgct
    24241 ggtatattgt ataatagtaa gaataatagt ggtccaaaga tattaccttc ttttgcaagt
    24301 gatactccta ctcctgctgc tataatccta aatgttcccc agaataatga atcaccaatt
    24361 cctgcaagag gtcccataag agctgtttta acagcactaa ttgaactttc atcaaattct
    24421 ggattattag cattctcttc ttccatagcg gctgttatac ctaatatagc aggaactacc
    24481 actgtcgttg tattaaaaat ctccaaatgc ctttctattg cttttgcttg attttctttc
    24541 ttattgtata gcttctttat tgcaggtaac attgaataac agtaacctaa gttttgcatt
    24601 ctttcgtaat taaatgcacc ttgaagagca aatgaacgcc aaaacatact tcttaattct
    24661 ttttttgtta atttattcaa agtcaatctc tccttccgtt aaagtattga ttgctaattg
    24721 aggattttga ttattttctt tgttattgat ataaaagttt agtacaaatg ctacacatac
    24781 tccaaataaa gctatagcag ttatatctaa tccaccataa gatgctaaca aaaaccctat
    24841 aaagaaaaat acagacatct tttttgaaag taacatatcc ataagcaatg caaaacctac
    24901 tgctggcaac aatgttcctg caacacccaa tccgtctaat ataactttag gtattgcatt
    24961 tattaatgct gtaactttat cagcaccaat cataattgct aaaaaggttg gaactgatgt
    25021 acttaaaaag aataatatta ctggcaacca catcattatg gtaactttct taaaatcagc
    25081 ctgtttagca tagaaaactg ccttttgaga aaagtacgaa tttattattc taactaatac
    25141 tccaagagat tgtgctaaga ctgctactgg tactgctact gctaaagcaa cttctgcacc
    25201 attacctgat aatatagcaa aagctgtacc taatacacct cctgttacta catctggagg
    25261 agtagctgca cctattcctg caattcccat ccaaattaat tcaagctgtg caccaatcat
    25321 aattcctgat tttatgtctc ctaaaactaa tcctactaac atacctgtaa ctattggcct
    25381 atcaagcata gattgaccaa atatacgccc atctagtata ccaactcctg ctataatagc
    25441 taacatagta gcttgtacta acatattgaa cctccatttt aacttagttt aatttacatt
    25501 aaattacatt acatttttga ttttgttttg attttctaat aactcattta cataaacaat
    25561 tccatttttt gcttcctcta atcctgattt aactattagt tttgtatcct cctcaactaa
    25621 taaaagtata gataaaagca ttggtaaatt tactcctgtc aataattcaa atttgaaccc
    25681 ttgtaaaagt aagtttgtac atatgtttgc cacacttcca ccaaacacat ctgttaagac
    25741 aacaaattct tcactatctt tattatttga taatatactt ctaatttctt tctcagcatc
    25801 atctgctgat ttatccgttg tcatacaaaa atactcaata ttagttaatg aaccaattat
    25861 taatttactt gtgtcaacaa gctctctgga caattctcca tgtgttgcaa ttagaaattt
    25921 tttcattctc tttctcctaa attattttat taaatctgat agttgtttct ttttgtctct
    25981 aggtaacatt ctaaattcta tatcaatccc taaatcacct atttgtttaa agttatttat
    26041 atcatcatca tcaacagcaa ctaaatctgt aagtttcttc ttcccttcct catatcttaa
    26101 tcctcctaca tttatttctt ttaatactcc tcctgaattt ttagctacaa ctaaagcatc
    26161 tttggtattt tttactaaca ctaatacatt gcttttcttt gaagaagcaa atttttctac
    26221 tatttctcca gattcctcta ccccccttat atataaagtt acattgctag gtttcgctaa
    26281 gttcagtgac atagctttca tttggtcatt ttttgcttca tcatttgcaa ctagtattac
    26341 atttatacct agtgatgttg cccatccata tgcaacctga ccatgtatca atctgtcatc
    26401 aattctcatc aaagatacca ttttccccaa atcctccatc atacattaaa atttttattg
    26461 tttatactct catttatcta taaaagcaaa aagtatgcca aatactggca tactttttta
    26521 aatttcattc acttattttg taatcttgta ttttactgtg gtagaccata gataatatta
    26581 taaatatatc ctatctctga aattggtatt tgcacactat aactcttttc tagcacacta
    26641 aatgcatttt ttattagttt tacttcacta tgatggtttt tgattaacaa atccatatca
    26701 gtatactctt caatttcagt ttgtctaact aatctttcaa tcatacaact taagtgaaca
    26761 tataaagaaa tctttttatc atttgaaagt tttttctttt gaatattttc tatagcatta
    26821 attccttctt ctattctctc tattatcttt tttgaatcta aaatagttag actatctatt
    26881 actctaatta atgaaaaatt ctttacaata gaatcattta tgttctttat tttttcattt
    26941 tctactactc tgctaaatat tctaaaaaca tcatcttctc cttttcctga tattatatcc
    27001 tctaataata taaaatctac cccattcaca tttggattgg cagttcctac tatagccatc
    27061 acatcataca tttcaaaaac tggttcttta tctcccatag aaacaagact atcataatca
    27121 taaggtatta ttttgacatc aactattcca attaaacttt cggaaagtag atgttggatt
    27181 tttatagcaa tacctatccc tgtaaaacaa gatgatatta tagcatattc tttatttttt
    27241 tgaggataat acaattgtat attaggttta gtttcttcat ccataatttc tgcaatctct
    27301 tcaatgtttt taccttgcat taataaattt cctacttcta gtgcagaaat agtggatatt
    27361 tggtctataa ataacattgg gctatttatc ttattcttta aatcttcata tataagatta
    27421 agagagccca tatcaaataa tacaattaaa ccattcttta tcctatctcc ctctagatat
    27481 ctagacattt gttttgatac atcagcaata gtagcatcta taggcatatc aatagctgta
    27541 aatatatttt tacccaacat ccgattacat acattcgcca tactactggc agtagcatat
    27601 ccatgtgcta taatagtcgc attaacttga ttaaatttca ttcttataat tgttgattta
    27661 ataaatataa tcattatgat tgtatctaca ttattaagat acatatccaa tctattcgtt
    27721 attaatgttg atattttact tattatagag tattctttgt aactctcttt ttttaaatat
    27781 attagtaatt tattttctaa agctttatac tcatcagaca attgataaga ttcgtccttc
    27841 aaataaagat aagacgaaaa tgccaataca ttattcccat catattttat attgttatta
    27901 acttctaaat ttctaaatat atcctgcatt atcgatatta taagctccat aagtacgtta
    27961 ttattttcat cattatttga aaagactaaa ttgtccatta gtctaatcat tagattagaa
    28021 ctctttttac taaatttttc ctttgttatt tcattttttt ctagtctatc aaagtaatct
    28081 aaacatttta tgaaactttg atagattata tttcttttat ttcttccatt tgacatttca
    28141 atactaaatt gtttaggaga aaagataatg cttttgcttt taccactttg gaattgaaat
    28201 atatctacat tttctatata aagatttcta ggaaggtctt gaagagtaac aaaaatattt
    28261 ccatttcttc tttgattttt tgcataagct cgtccacatg catattttat tacattttta
    28321 cattcaccta tattcccctt gaattttatc tttaaaatcg catttaatgt aatttgagtt
    28381 atctctatag gcaaaccaag taccttactt tcttcataaa taaaatgttc tacaatcttt
    28441 cttttttcaa tagtccctct ttcttctata ttgggaacat aaatatttat gggtattctt
    28501 cttaaaaagg tttgtaaaaa ttcagataga ctttctgttg tagcaaatat taatcttacc
    28561 ctagcttttc taattactcc attctctcca atcctagaaa acgtacctgt atccataaat
    28621 gtaaataact tttcctgacc ttcactattt aatctatgga cttcatctaa aaataaaaca
    28681 ccaccatctg attgttctat tagacctgct ctagattcat ttgctccagt atatgctcct
    28741 tttacatgcc caaataatag actagacaat aactccaaat tattataata ctgcgcacag
    28801 tttaacacat tgtatggaga atttttttct aatacattac tgcttacact atatttgtat
    28861 attatcttgg ctaaaaaagt ttttcctact ccactttcac ctgtcagcat tattgtaagt
    28921 ccgccatttg gataaagtac agcggttttt atttgttcga ttacttcttt taagctatta
    28981 tcatatccta tgatactatt aaacacagtc tcttgcttta ataatttaca ctcattttta
    29041 agttcatcta aattttcata aatacttttt tttgtaggtc ctactacatc atttatttta
    29101 tttttaggta aaaaaataac aggtctactg tttatcttta taagattatc agattttact
    29161 aaatcattta ataatctact tgattgagtt ctacttaaat taaacttttt tactatatct
    29221 tctgttatta tgccactttc ttttttaagt tcatcaaagc tgttaaataa tacacattca
    29281 tttacaaaac ttaaaatttc atccctcatg ttcaacctcc aatacaaata tcaataatac
    29341 tataatatta gtatatcatt tatatattta tgaaaaagat aattatattc ctatcttgat
    29401 tctcttttgt aaggttgtaa aatcaaactt tttataagta taaaatctag attttttaac
    29461 ataatctcaa ggttcttatc tttccatata tctgacata
//
LOCUS       DAMJWU010000039        29409 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMJWU010000039 DAMJWU010000000
VERSION     DAMJWU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.039"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            130..438
                     /locus_tag="PC460_001934"
     CDS             130..438
                     /locus_tag="PC460_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600820.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(600..2390)
                     /locus_tag="PC460_001935"
     CDS             complement(600..2390)
                     /locus_tag="PC460_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600821.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(2390..4117)
                     /locus_tag="PC460_001936"
     CDS             complement(2390..4117)
                     /locus_tag="PC460_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600822.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(4362..4628)
                     /locus_tag="PC460_001937"
     CDS             complement(4362..4628)
                     /locus_tag="PC460_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HDF2600823.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            4926..6038
                     /locus_tag="PC460_001938"
     CDS             4926..6038
                     /locus_tag="PC460_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2600824.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(6402..6911)
                     /locus_tag="PC460_001939"
     CDS             complement(6402..6911)
                     /locus_tag="PC460_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HDF2600825.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVLVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(6913..7560)
                     /locus_tag="PC460_001940"
     CDS             complement(6913..7560)
                     /locus_tag="PC460_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600826.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(7574..8746)
                     /locus_tag="PC460_001941"
     CDS             complement(7574..8746)
                     /locus_tag="PC460_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2600827.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPAICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            9014..10390
                     /locus_tag="PC460_001942"
     CDS             9014..10390
                     /locus_tag="PC460_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600828.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILSNYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(11327..11857)
                     /locus_tag="PC460_001943"
     CDS             complement(11327..11857)
                     /locus_tag="PC460_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2600829.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(11945..13039)
                     /locus_tag="PC460_001944"
     CDS             complement(11945..13039)
                     /locus_tag="PC460_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF2600830.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADRNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(13041..13487)
                     /locus_tag="PC460_001945"
     CDS             complement(13041..13487)
                     /locus_tag="PC460_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2600831.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            13793..14983
                     /locus_tag="PC460_001946"
     CDS             13793..14983
                     /locus_tag="PC460_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2600832.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            15153..16115
                     /locus_tag="PC460_001947"
     CDS             15153..16115
                     /locus_tag="PC460_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2600833.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(16237..16899)
                     /gene="zmp1"
                     /locus_tag="PC460_001948"
     CDS             complement(16237..16899)
                     /gene="zmp1"
                     /locus_tag="PC460_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF2600834.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYAEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(16999..17084)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(17270..20188)
                     /locus_tag="PC460_001949"
     CDS             complement(17270..20188)
                     /locus_tag="PC460_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2600835.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(20306..20391)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(20518..21696)
                     /locus_tag="PC460_001950"
     CDS             complement(20518..21696)
                     /locus_tag="PC460_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2600836.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            22071..22220
                     /locus_tag="PC460_001951"
     CDS             22071..22220
                     /locus_tag="PC460_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2600837.1"
                     /translation="MFFISYIYTKSPIEFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            complement(22321..25095)
                     /locus_tag="PC460_001952"
     CDS             complement(22321..25095)
                     /locus_tag="PC460_001952"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2600838.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(25267..26523)
                     /locus_tag="PC460_001953"
     CDS             complement(25267..26523)
                     /locus_tag="PC460_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2600839.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(26595..28097)
                     /locus_tag="PC460_001954"
     CDS             complement(26595..28097)
                     /locus_tag="PC460_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2600840.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(28188..29378)
                     /locus_tag="PC460_001955"
     CDS             complement(28188..29378)
                     /locus_tag="PC460_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2600841.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         9650 a         4976 c         3497 g        11286 t
ORIGIN      
        1 taatcagaaa gttatcttta taaattatag ttttagattt ataatttata aaatatttat
       61 gttctttgta aaatttttta gaagcttcta atgttatgct ataaaactat cttgagaagg
      121 gctgattgaa tgtttaataa aatatatgaa aagaaagaat ttatagtttt tcaagttaaa
      181 aaaggttatg tagtttataa tactagaaag agttttgaag aaggtcatac acatttaaag
      241 cattttgaag cagcaaagac agctattgat ttagcaataa ataaaaagat tcctagaagc
      301 aaagatggat attatctgac tagtttaatt cgcataagtg atgatggtta ttacattgat
      361 aagttaagcg aattgttata tgttagagaa caaaaaggta aaaaagaaaa gtactgtaat
      421 tctggttatc agatgtaaca agactagttg tgaattataa gtaccaatca taagtacctt
      481 tggtcttaaa atcaaagagt atcagtaaat ctataaaaaa acaacttact gatactcttt
      541 ttaattatat gattaatatt tgaatttgat acaaaatttt atacaaaagt ttatgttatc
      601 tattctgatt ctttgtctgc aaattggcta ttataaagct tctcataaaa tccacccttt
      661 tccattaatt catcatgatt cccctgctct ataatacttc cattattcat aactaaaatc
      721 aagtcagcat tgcgaattgt agaaagtcta tgtgctataa caaaactagt tctaccattc
      781 ataagatttc tcattgcttt ttgaagcata agttccagcc tagtatcaac agaactcgta
      841 gcttcatcaa gtatcaatat tgaagggtct gatataaaag ctctagctat ggttaaaagc
      901 tgtttttccc ctagagatat attagatgct tcttcattta aaaacatatt gtatccatct
      961 ggaagagtgg ttataaagtg atgaacattt gctacttttg ctgcttgtat aatttcttct
     1021 ttagtagctc caaatctacc atattctata ttttcaaaaa tggttccatt aaataaccat
     1081 gtatcttgaa gcaccatacc aaacattgaa cgcaaatctt tacgtttcat atctcttata
     1141 tctactccat caattttaat agcacctttc tttacatcat aaaacctcat caataagttt
     1201 attaatgttg ttttacctgc tccagtaggt ccaacaatag ctaccatctg accacttttt
     1261 acttctgcat ttaaatcttc tattaaagtt gaattttctc catatccaaa attaacatgc
     1321 tcaaatgtaa catttccttt tacattttct atcttaactg gattttcaac atcaggtatt
     1381 tcctcttctt cgtctaaaat ttcaaataca cgttctatag ctgcaaaaga agattgtatt
     1441 actgtagata actgagtcat ttgtgaaagt ggctgattta tctgccatat ataacgcaca
     1501 aatgcttgta aatttcctac tgttattcct ccacttactg catatataga accgacaact
     1561 gccacagctg caattccaaa gtaagttact aatgaaacca taggattcat catacttgat
     1621 ataaattgtg ccttaaatcc attttcacaa agttcttgat ttactttttt aaattctttt
     1681 atagaatctt cttctttacc atatagtttt atttcattaa atccagtata catttcttgt
     1741 actttcccat ttaattttcc aagagcattt tgttgcaatg aaaatctact ttgagatttt
     1801 tttactacaa atttagatac aaaataacta attggaatta taaaaagagc tataagagcc
     1861 atatatatat taattgtaaa catcattgaa agtgccaaaa ttatgactaa gattgagttt
     1921 acaatttgca taaaactctg ctgcaatgca tttgatattg tatctacatc atttgatata
     1981 cgacttaata catctccaaa actattacta tcaaaataac taataggcaa tcttcttatt
     2041 ttattttcga cttcatttct taaatctctc attgtgtttt gaatagcatt ggtaagtaga
     2101 aaacttgaag tatacgtaaa aattgcattg gcaaaatata tacatgctaa aatcaccaat
     2161 atttttgtta cataatcaaa atttacggaa gcccctggta ctccttttgc gatactaatt
     2221 acatctttag tcaattgtgt tattatcaat ccttcagttt taggtgcaag tgcattcata
     2281 gtagctgctg aaattataca tataatagct ataataaagg aaaatttata tttctttaca
     2341 aatggaagaa gtctaccaat tgttttctta aaacctttag actttttcat tattctaatt
     2401 cctccttttt taactgagat gttgcaattt catagtatat ttcacagttt tttaataatt
     2461 ctttatgagt tcccattcct acaacttgtc cttcattgag tactataatc ttatcagcat
     2521 caataataga actaattctc tgggcaacaa tcaatacaat tgcatctttt gtttccttct
     2581 tcaatttagc acgaagtatt gcatcagttt taaagtcaag tgcagaaaaa ctatcatcaa
     2641 aaatgtatat ttcaggtctt cttataatag cccttgcaat agaaagtctt tgtttttgac
     2701 ctcctgatac atttgctcca ccttcactaa tcaattcatc aaactttcta ggtttttttg
     2761 atataaactc atatgcttgt gcaacttttg cggaatattc taattctgac tctcctgcct
     2821 tatgtttccc aaatctaata ttatttgcta tgctaccagt aaaaagcagt gatttttgag
     2881 gtatgaatcc tatctttttt cttaaagctt ttaaatcata ttctcttata tctactccat
     2941 ttatcttaat actaccttct gttacatcat aaaatctagg tatcaagtta attagagtac
     3001 ttttcccact accagtactt cctataaaag caactgtttc acctgttttt gcagtaaatg
     3061 aaatatcctt aagtacagaa gcttctccat ctggataggt aaaagttaca ttatcaaatt
     3121 ctatgatacc cttattttca gtatccttaa ttccattttc aggatttttt atcaatggtt
     3181 cttcatttaa aagttctttt attctatttg cagatacctc tgctcttgga tacataataa
     3241 ataccattga aaaaagcata attgaaaaca tggcatggaa aagatattcc aaaaatgcaa
     3301 ccaactgtcc aacttgaagc tctccaatat ttatcttctc acttgaaatc caaaatacag
     3361 ccaaaacagc tatatttaat agtaaaaaga atgctggttg agtaattgac ataagcttaa
     3421 atagtttctt agaaacatta gcataatatg tatttgtttt ttcaaatcgc tctgtttcat
     3481 aatcatcatt tccaaaagct ctaattactc ttattccagt taaattttct ctcgatataa
     3541 ggtttaattt atctagtcct ttttgttgtt ttgaagatat tgcctgagat acttttgcta
     3601 ttattgctac tccaataatt ataaaaggta ctgatatagc caatactata gaaagttcca
     3661 cacttgttct aattgtcatt attatactta taatgaacat aactggagtt aataaggcag
     3721 tccttagtag tatattaaca aattgttgaa tttgaaatgc atcattcgtt gttcttgtaa
     3781 tcattgaaga aattccaaat ttattgtatt cagtatgtga aaattcttgt gatttctcaa
     3841 atatgtcatt tcttatatct ctggttacac ttgttgatac cttagcagaa caataaccca
     3901 acaatatact acctacaacc cctataatag aaataataac aatcactatt cccatttgtt
     3961 ttatgtaagc tatattctga tttgcaatac cattgtcaat tattttagct attatagttg
     4021 gaatccccaa ctcgactaat gcaaaactaa atatagccag tatgtttaat actattaata
     4081 acttataatt ctttaaataa cttaaaatta atttcatatt tttctccttt cttaattttt
     4141 gagatagtgt aatcatgtaa taatactaaa tttataaatg cttaatttta taaaaaaagg
     4201 agaaaagagt taacgcactc tcttctccag catattcttt ttaatacacc ctccgacaag
     4261 tcgcttatat gttaacatat ttgacaagta atgtcaattc atatagccaa aagtctttta
     4321 aatgcttcat ttcataacat tgtaacaaat tggcatgtaa tttatttttg ttttttactg
     4381 tattccaatt ttacaagttc tccaatatta ctagctttct taaaattcaa attagtctta
     4441 tctataccag acttaaatag tgaaatccct ctccccaata ttattggcaa tatatatata
     4501 taataatcat caattaagtt atctttcata aataatttta ctatatcgcc tccaccaact
     4561 atccaaatat cttttttatc cttctctttt ccaatttgct caattaattt tttaggttct
     4621 aaattaattt atagtgtgat tattctatgt aaacatcact tttttactca ttcaataata
     4681 ccctattaat aaatcctcaa actaatttaa ataaactttg cataaaatta aaaatatatt
     4741 atataaataa tttaaaacca cactaactta ttgaaaattc taattattac aaataaatat
     4801 tgctctctgt taacgctagt tctataatat aattataagc tattaatatt ataaagacga
     4861 tattactaat ataataatta taatacttat agttaattta cttttgttga aaggtggtaa
     4921 ataacatgaa aatttcagaa gtcgctactc caaatttttt gctagattta gaccagttag
     4981 aaaagaatat agataaaatt caacaaattt gtacaaataa taataaacaa ctttggccta
     5041 tgcttaagac acataaaagt acttacatag cgagacttca aaaaaatgca ggtgctagtg
     5101 gttttctagt tggaacactt gatgaaggtg aagctctgat aaaagcaggt tttgaagata
     5161 ttatgttggc ttatccttac atgggagaag taaatataaa tagaattgtg actatggcat
     5221 caaaaataaa tttaatctgt gctacagata atatagaatg tgctaaggct tatagtgaag
     5281 ctttttctaa atcaaaaata gagtgcaagc tacttattat tgtagattgt ggcttacacc
     5341 gttttggggt tgaaccagag aaagtggttg aacttgctac tgaaatttct aagctagaaa
     5401 acataaagat tgttggtata agttcgcatc caggtcaagt gtatggttgt agttctccag
     5461 aaggtgttcc agatgtttgt gttcaagaag attcttctat ggagaaagca tacaaagctc
     5521 taactgataa tggatttaat atagaaattg tcgcttctgg tagtactcca acagttgaat
     5581 ctgaagctgc ttctaaaact ataactgctg taagacctgg aaactatgta tactatgatg
     5641 ctatacagat aggtttaggt tgtgctactg aagatatgtg tgctcttact gttgtgtgca
     5701 ctgtaatctc aaaaaatagc agtggatact atttaattga ttgtggaagt aagtgccttg
     5761 gacttgataa aggtgcacat ggaaatagta gtattgttgg ttatggtagg gtatgtggac
     5821 atccagaact tactattgat tcactttcag aagaagttgg aaaaattaaa gtaaatggtg
     5881 aaactgatat aaaaattggt gatagaataa gaattatacc taatcattct tgttccactg
     5941 caaacttgac aagtaattta ttaggtttta gaggagatac aatagaaaaa gtcatagaag
     6001 tagatattcg tgaaaattca aaacaaatca tcctttaaaa acagatttat tctgatatta
     6061 acatgaaagt atatttttct taaaattatt tctaataact aatgttaatg ttggataaat
     6121 aaaaaacata aattctagaa atataaattt aaaagaagag gctgtctcaa aatagagata
     6181 agcctctctt tcattgttga aattatgaaa atatacttga tttcatacaa aaagagtatc
     6241 tcatgaacta aaaagttcac tttgagatac tctctttttt taattaatat acacattaat
     6301 tactatctta gtttattact atatatcaat tactactcat taatccttgt atatattaat
     6361 tcttatatat catttatagt taggttataa ttttatgtta tttacattaa tcccataaca
     6421 gtgagtaaaa ctcttactat taacatcccc actattgcat caaatatagg aactgccaac
     6481 agtaacattc tatacttagt actggcattt gctacaagca ctattctggc ataatgacca
     6541 actagagtac ccattaacat acatggcata attaatatgg ttgcttgtgg tgcagtgatt
     6601 agaccttgtg caaataagtt tgcagctgta gctgctcctg ctgccttagc aaagaatgct
     6661 gctatcaaaa ctaaaacaga ttccccaggt agtccaaaaa tacccattac tggtccaaaa
     6721 atatttccta aaatatcaac taaaccagtt aattgtaaaa actgcactat tacataaccc
     6781 attatcatgg ctggtaatat ctgttcaaca cctatgtaaa aacctttttt tgctcccccc
     6841 ataaatatct caacagcagt tttcttaggt ttactattta caattgcatt tccagtattt
     6901 tgttgactca tactacacag cctccttagc cttattcttt ttatttctta cacctatgat
     6961 taatctaact agattagcac caagtatttt acagaatatc tctataagga ttattggacc
     7021 taatgccaat aatgctattg gtaaaagtgg agcttgagta tttattgtgt tcagtataac
     7081 agctgaacca gcatattgat atgctacaaa tatagttctt tcatcatctg ttatttcccc
     7141 actttcaaat aattctttcg tcattattga ggctacatct gaactagtaa atgaagatac
     7201 aaatgctatt cctgctattc caggtatacc aagcagaggt ctaagcaatg gattaaatat
     7261 ctttgatgcc gctttcattc ctcccataga ttcaactaca tctataagac ctacagcaaa
     7321 acagacagtt ggaatcaaag tcagagcaaa taagaaacca tcttttgctc ccgttcctcc
     7381 tttacccata aagtctacac catctgcaat agtaccaaat gaacctgtca gattagcaaa
     7441 atctaatgct ttaaggaagc cttgactatc tttaaacact cctgaaaata atactatcaa
     7501 tacaaataaa gataaatacc ctttccatgt taccttatta tctccagctg ttacatccat
     7561 ataaaatccc cctttatacc tcaaatttat ctaagtttaa aataactgta gaaattaatt
     7621 tcaccctctc aaacatagaa tctactactg catattctct tgatgtgtgg ttccattccc
     7681 ctcttacacc aaatgaacag attgcgggaa cgtttgcaat agtcaaataa ctagcatctg
     7741 aactacctcc aagtctctta gcatttaatt ctccaaatcc attttctttt gacacttcat
     7801 ttacaaatgt atgaaatctc ataacatctt ctgtagtttc aaatggcatc atttcactta
     7861 taaaattaac ttctgtagaa gttccttcta tgtaagtttc tttacatatc tctctaactt
     7921 gttctttaac attctccatt tcttctactt tttcaaatct aaggtctact tttatactac
     7981 aatcttctgg aatagaattt tccactcttc cacccttaat cacacttaca cttactgtag
     8041 ttccaacatc taaattagtc aatttttgta tgcgtagtat cttattagcc atttcttcta
     8101 tagcatttct tccaccttca aaatcattac ctgcgtgtgt ttctacacct tttacatgta
     8161 tattacatcc tattcttcct tttctaccta cacacaaact attatctact aatccagttt
     8221 ccatattaaa agcacaaaga ccaccttttg cttccctcaa tataacatct gcacctgttg
     8281 aatccttatg tcctatctct tcatctccag aaaatactat ttttattggt ctttctttgt
     8341 atcctatttt atttaatgct ttaataacat ataaggaaat tattatacca cccttcatat
     8401 ctaaaacacc tgggccatat gcttttcctt ctattatttt aaatggattc tctccaaatg
     8461 tacctgtttc aaagacagta tccatgtgac ctgaaaaaat tataggcttt ttatttatat
     8521 tagaaccaag tgtacctatc aatgtactac cattatctcc aacatctact aactcgcaat
     8581 ctaaaccttc tttttcaaac tcaaacttta atctcttagc caatttattt acgctttctt
     8641 tacaatgtgt atagctttct atattaacaa tctctttcca taatgaaact atttcttctc
     8701 tattctcatc aacaaactta gaaatttcat ttaaatatgt attcataatc ttctccttta
     8761 ttttcaaata aaatgtattt ctttcctaaa atctaagttt attttaggaa atcttaaaag
     8821 actagtcctt aattacaaaa tagcacaatt attcttgaca gtcaacatat ttttcagtct
     8881 tttttatatt ttctgatttt tctttaaatt ttccttttgt aaaataaatt gacgcgtttt
     8941 atctactatt ataaaatgag gttataaatt gattgattag ttactatact ctcgaaattt
     9001 taaaggaggt tttatgaaat ataaaattgg ttttttgaca acatcttatt ttatgtcaga
     9061 taagtttaaa caagctctta aagaattaaa tgatacttgt gaaattacat ttattgagct
     9121 caaaggggag aatcctttta ataatttacc taatatatac tatgagcaag tagataattt
     9181 tgatggattt gtatgcagtg gtatagttcc atatagtgaa cttataataa atataaaaga
     9241 cataaaagtt ccattaaatt ttttaaagct tgatgaaaga gatttttata aatacttatt
     9301 caaattatta aatagccaaa aagatattga ctttacaaaa tcatttatgg attttttaag
     9361 agaggacaac aattattatg atatatattc attaatagac tctaataaat gtccatatac
     9421 aattaaagat tttaatatcc ctcaacctgt atatgacttt aaaaaattaa atgataaatt
     9481 attggaaata catttagact tattgcgaga taataagatt aacctttctt ttactagaaa
     9541 ttacatcatt agttgtgaac ttaataaact tggatatgat tgtatttaca tggttccatc
     9601 tacagaatca atcctgaata catttaaatc tctggtgaat gaaataacat tacaaaactt
     9661 agacaaaaat aaatctgcaa cttgtatagt tacagtaaat agttcagagt acataaatga
     9721 agatttgata tttaaaaatg atgagataca aaatcaaata tataacacta tattaaattc
     9781 tctatcaaga catggatttt atggagttca ggtcaaaaaa aatgatatga aaatagagat
     9841 acacactact aaagaaatgt taaacaatat gaccaataac tatacagatt tttttatttc
     9901 agaagattta aaagaaatta aatatagctt aaatataggt tggggcattg gaaatacaaa
     9961 tattcatgca gagcaaaatg ctcgtcaagc taatggtaaa tctgcttctt tgaatggaaa
    10021 ctgtactttt atagtaaatg atactaatga tacaataggc cctttatatt caaataaaaa
    10081 ttctaataat attgaagata gctcaatagc caataaagtt gcttctttta tacctctatc
    10141 taatacaaat gtatctaaaa tcatgtgtat gattaatgat agaaattcaa ataatgtgtc
    10201 agcagaaata ttatcaaact atatgaacat aactcttaga agtgctaata gaatattgag
    10261 tatcttatat aaagcaggaa ttgcaactat tgtaaataca aaactcgata atcaaagagg
    10321 aaggcccaaa aaaatatata aagttgattt cttatctttt ctaaataaga tgcagaaatt
    10381 aaattcttaa tatttaattt tttaataaga acaatgtatt ttctctaatt ttaaagaatc
    10441 aaaatgttta aattttatta gaatatacaa ctttttactt taaaatacct aaacctttta
    10501 tcagtgaaat aaaccataat atatcgtaac ttattagaaa aacaaaataa tttcatatac
    10561 aaataataaa atagccacta caatccataa tttgcagtgg ctacttttta cactatcact
    10621 tattacttaa tttttaaaaa ttaattgtat tttttaaata atttatacat acctattatc
    10681 agtaaagcaa taaatacaag tatatctata aaaataatta ttgtaacatg tctggcaata
    10741 ttgtaaataa aaagtataga gaatatttct atagacaacg ctacaaaaca cataaaattc
    10801 gttaaataat ctgcaatagt gtcactataa aaagcatgta tttgtaacca tctccatata
    10861 aatggacgta aaaggattct gaatacaaca caaatgacag ggtaggcata aataaatttt
    10921 ttatctacat aattagctac accattattc cattggatag gaatttgagg tggcaacttt
    10981 ggaaatactg ttattgcaat ttaaacacta cctctctatt ctattaattt ataactataa
    11041 tatagtctta aatattttca ctctataaaa ttgtatattt agtatatctt aaaatcaaac
    11101 taattatctt ttataagttt tcatacaata atatctcctt tcaataatat atcttttttt
    11161 atatttaaaa ctatcttatt ttctataaaa taatgtataa acaaggttta cagtcaagta
    11221 tttttattat ttttttgatt tcataaaaaa agcatagcaa attatttgct atactttttt
    11281 actaaattat ttattctata tcttatatga acttattgtt taactactac acaatactag
    11341 ctttaatcat catatttttt gcaaacatac tggcttcata tatttcttgt tcatctggat
    11401 gactttgaga ttcctcatgg ttatttaatt gttggcgaat atgttcgttg tcaggagaaa
    11461 gttttaacat ctctttatat tttgctttca atccttctgc tatcttacct tgacatatga
    11521 atgaacctaa tatttcatta tcttttggga taattgattc tatcttttct attatactag
    11581 ctccataact tcctttttta cttgctccca ttgttccaaa tagtgctatc tttttattgt
    11641 gaatattttc aagcacttgt tttgaatttt tatcacaaat tcccttgtcc acccaatagc
    11701 ctaagaatac tatatcataa ttttcatagt caatactata atcaactaat tcatttacat
    11761 ctttaatgtc tttttcttct tgaatttcat catatatagt ataagcaatc tttttagtat
    11821 ttccagttaa acttgaatat aaaactaaac ttttcatgat gtctcctcct ctttatctaa
    11881 atacttatct aaatcaccaa aaatatatta accttaacaa attatttatt tttataagga
    11941 tattctaatc tagtatacat ccaaactgag agcgtgtttg taaagcatct gcacacttct
    12001 tattacaata acagcacttt ttaatctcat ctactcttcc atcttttact ttctgtaacc
    12061 aattcctatc tgctagtaat tgtcttgaca tagaaaccat atctactcta ttatttgaaa
    12121 taatactttc tataaaatct gggctagaca attttccaac cccacatact ggaagatttg
    12181 tgtgtttctt tacttcatca gctaaatata aaaaacagcc ttcttcttta aagtaaggat
    12241 gattattggt tggtatagta tcttctaatt ttgaatgatt tgcaagtgtt acatgaaaac
    12301 tgtttacacc tagggattca aattttttaa caaaatactc aacttccgaa agtaaaactc
    12361 ctgcatttcc ataatgagga ttttcttgtc ttattgcaaa tttatagtct attggaatgt
    12421 ccttaacttc atctcggata ctactaatta tttcgcttgc aaatctactt ctattatcac
    12481 acgttctacc atattcatca tttctattat taaatatact tgatgaaaaa cttcctacaa
    12541 gcctatcacc atgaatttga atcatgtcaa aacctgcttt ttttgcattc aatgctgtta
    12601 ctttaaataa atttataata tttttaattt cttctttggg catatttgtt atgtaatctt
    12661 ttactccgtc attcattatt ttttttatct catcatgtga agtaattccc atattcatag
    12721 tatctttaat taaattaaca tcataatctg aacaaaatag ttgtgctgat acctttgcac
    12781 cagaattgtg tatttcatct acaattttct tgtatttcat gactccatct tcatccgata
    12841 ggcttatagc taccttatga aaactaggtc taatagatac atctcctaga gtaatcagag
    12901 ctactcctga cttagcaata tcagaaaact ttttaatttt ttcttccaat ttaagaccca
    12961 tgcttgtagg tgcaaaagat actctatttt ttaattccaa atttccaatt ttaattggtt
    13021 ttaataaatt acaatacatc ctattcttcc tccacagctg tttttatatc ctttaccaca
    13081 tgtaaattct ttaccagttc cttatattct gtatccgaga actctttttt tatgtgatta
    13141 tcccactttg aaaatatgtt ttttatatcc cctaatactt cctcaccctt atctgttact
    13201 ctcaactgaa aagctctact atcattaggg ttttggattt tttctatgta tccatcttgt
    13261 tctaacctag atatagttct tgttgtatga gctttatcaa cttcaaattc tgtagaaaca
    13321 tcattcaaag aacaattcct atttttatta atataaatta aatagaaata tagaccattc
    13381 gtaagtttat atggttttaa acatttttca caataaagtt taaagttttt ttgcaagtta
    13441 caaataatat atgacaaact ttcatactgt aaatttttaa aatccatctt aaatttccct
    13501 tctataatta taatatgttt gactatatca actatattat attaaacaat taatatagtc
    13561 aagaatattt tattctctaa atctatgttt tattaattct ttaaaaaact attatataaa
    13621 ataatcatga taaaattaga aattggaatt tttaatatgt actgtacata aatatatttt
    13681 atataaaagt ttatattttc aaacaagtaa tttttcgaaa attgtctaaa ttcagtaaaa
    13741 atatggtaat atatacataa gcaatataaa tctataaaaa ggagtgttac atatgatatc
    13801 taataaaatg caaactttag ttgcaaatag ttctgttata agagctatgt ttgaagaagg
    13861 aaagaaactt tctgatattt atggtgagga aaatgttttt gattttagta taggaaatcc
    13921 aagtgttgaa cctccagaga ctattaaagc agttataaat gatattttaa atgaagaatc
    13981 tccaaattta gttcatggat atatgaacaa ctcaggatat gaggatgtaa gagatgctat
    14041 tgctgaacac ataaataaaa aagatggatt aaatcttaca agagaaaatt taattatgac
    14101 ttgtggtgct gctggtggtc taaatattat tttaaaaact cttttaaatc ctggtgatga
    14161 agttattgct tttgcaccgt attttggtga gtataaaaat tatacagaaa actatgatgg
    14221 aaaattaata gaagttccta caaatataga gacttttgag ccagatttag atgctctaaa
    14281 aaatgctatt actccaaaaa caagagcttt aataataaac actccaaata atcctactgg
    14341 tgtcatatat tctgaagaat tattaaaaaa tcttggagaa ttattggatt caaaacaaaa
    14401 agaatttaat acaagcatat acctaatttc tgatgagcca tatagagaaa taatttatga
    14461 tggtgccaaa gttccttgtg tactaaaata ctatagaaat tcatttatag gatactctta
    14521 tagtaaatca ttatctctac ctggtgaacg tataggatat attgttgcta atgggcaaat
    14581 ggatgatttt gatgatgtta tgtcttcatt aaatgttgca aatagaatac ttggatttgt
    14641 aaatgctcct tctctttttc aaagggttat tgcaagaagt ttagatgcag aagttgatgt
    14701 aaatatttat aaaaagaatt ttgaccttct atataatagt ttaatcgata tgggatattc
    14761 ttgtgttaaa cctaatggta cattctattt gttcccaaaa gctccaattg aagatgataa
    14821 aaaattctgc aatgatgcaa aacaatttaa tttattatta gtaccaggtt cttcttttgg
    14881 ttgtccaggt cattttagag tatcatattg tgtatcttat gataaagtta aaagttcttt
    14941 accagcattt gaaaaattag ccaaattata taatttaaaa taactaaatc aaagtaattc
    15001 aaataattaa ctaccttagt atagaatact tacattctat attaaggtag tttataattt
    15061 tatgtactat attttataca taccatatta ttccaatatg cttgaatatt cttatctata
    15121 gtactaatat aaattacaag gaggtgtttg acttgaaaaa aggttatgtt tatataatac
    15181 tgactacctt attttttagt tcaatggaaa ttagtctaaa aactgtcact aatgacttta
    15241 atcccttaca aataacttta tcaagatttt ttgttggcgg tttagtctta ctaccttttg
    15301 ctgtaaaaag attaagagct cttagtcttt ctattacaaa atcagactta aaatactttg
    15361 catttttagg ttttatgtgt gtagtagtaa gtatgtcatt gtatcaactt gctgttctaa
    15421 acacaaaggc atctgtagtt gctgttttgt tcagttgtaa tccagtcttt gtaatgcttt
    15481 tagcttactt aattttgaag gaaaaaatat ataggcacaa tgtaatttca cttatattag
    15541 aagttcttgg aattatagtt ataataaatc cattgcatac aaaactaact cttagtggaa
    15601 tattcttaac attatcttct gctattattt tttcaatgta tactgtattt ggtaaaagaa
    15661 aaaccttaaa atttggtgga atagttgtaa cctgctttag ctttatattt ggtagtttgg
    15721 aaatgttgat attggtttta ctcactaaaa ttaattttat cgcagatata ttaaataaaa
    15781 ataagcttca tgtatttgca aatatcccta tttttagtgg atatacctta cacaatatgc
    15841 ccattatgat ttatgtattc gtatttgtta caggtgttgg ttatgcgctt tactttatgg
    15901 caatggaagc tacatctact agtcttacat ctttagtatt cttctttaaa ccagtaatat
    15961 ctccaatact agcattatta atacttaaag aaattatccc tataaacatg gttataggaa
    16021 ttttattgat agtaattggt tcaataattt ctattatacc tacgataatt actcaaatgc
    16081 ataataaaca cgttgaagcc ttgtataagg agtagtaatt gataatctat catagttata
    16141 cataagacaa aatttattag atttaaacgc aatataaaag agatgctatt ataatagcat
    16201 ctctttattt atatttattt taaatttatt aaaactctat ttagctaaat tttgcaaaaa
    16261 gctataagtt tgtggacaag ctgattttaa cttactattt gtatcttgat ttaaatagta
    16321 atatgcaaat gattctgcaa aaaattcttc tgcatataca cctaagtagt ttacattacc
    16381 taaagaacga ccttctttag caaatatttg tttgaattgt gctgatttag atatatcatt
    16441 taatactata tggtctattg catgtgctgt ttcatgtaat tctaaattta ttgcatcatg
    16501 ccctttacct ttgttactaa atcctattct aagagctact gtacttccac ctaaacctgg
    16561 aacatcatcc caagtttttc cagtaccttc ccatccctta ggaactacac cttttaaata
    16621 agcatattct ttttcatctg ttaactttcc acttaataat tttattttta agttattttg
    16681 ctttaatgca tttaaatact ttccatctat attagctaat ctatttacca tagcattagc
    16741 ttcgttttta tcgtaatttc cagttggaaa aactactatt tgactaagtg tgtctttatt
    16801 ttgttgtata gtagtactat ctgcatgtgc acttactgga actgaactta ttaagaatat
    16861 tgatattata gctattaata attttttact tggtctcata ttattccctc ctttgagtta
    16921 ggccgggagt aataaatttc aaaataaaaa agatacccaa gagtatcttt gtatatacaa
    16981 aaagaaattt ttactctttt cggcaactgg ctggcatggt ttactaaact ttagccccta
    17041 tagctttgcg tgactaggtt tccctagttt tgcctcattt aattttatat ctatattata
    17101 gcttatatct ttttaattgt ctatatccct ttggaacttt tttctaaaaa gttattattt
    17161 ttttacataa atcttcaaaa aaaaatatct tctaacaata ttttcattat taaatattgt
    17221 tttagaagat attttttaat ataacttata ttttttataa tgttagggtc taatttgtat
    17281 ttttatttct tcttaatacg ataagtccta caatagctcc tataaccaaa agtatttctc
    17341 caataattgt agtactatcc cctgtttttg gtggtactgg tggatttacc aaagtatcat
    17401 ctttaacttt attatttttt tcttctgaca ctgatggctt tactacttct ggtattggaa
    17461 taatctgttt aacaggagtt tctatcatct cttctgatgg tggtactggc ggattcaata
    17521 cctctggtat tggcggattt attatatctt ctgatggtgg tattggtgga tttataacta
    17581 tagtatcctc tgtatcagat gaacttgatg gtttccttgg tttatctgtg cttggtggta
    17641 ctggtggatt tactattggt tcatctgtat ttggtggtgc tggtggattt actggtggat
    17701 tttcttgtct tatgttttct atatttccaa tatccactat tctaccaata tctttattac
    17761 caatttcaac cttaatttta tccttcagtt tttcatatcc acttggagcc attatctcca
    17821 ctaaataata tgttcctggt ttttttatac tagattgaat tataccatct tcatcactaa
    17881 ctaaatcctt aactactata tttccaaatg catcttctaa attgaactta gcacctgaaa
    17941 gtacattttt atctaaagca tccacttttt tagcttcaaa agcataacct tgcttagtag
    18001 tctctactcc tggaggactt gaaccattaa tctgagccac attttgtata gtctccccaa
    18061 cttttacatt tgtgaagtct gcatcaaatg taacccaact ttgcacaggt tccccttctt
    18121 ttatctgttt aaattcgtat ttatttacta atcttattgt attttttcca tctccagctt
    18181 cttttttaag ttcaagatta tcagaatatt ttatattaga tatttctaga gctgagttta
    18241 cttcatctaa aagatttact tgtcctgcat cataaactcc ataagttgac atattgattt
    18301 tgtatttaac aatctggtta tcactactat ttaaaacttt tttatcaact tctttttttg
    18361 cgcttattaa tccagaattt ttcttaaagt ttatctcatc atttttatca atttcactat
    18421 tatttgcatc aagaacagct ctatttatat agctatcttc aatactaatt acctttaatg
    18481 tataagctat aaaaaacttt gattccactt cttctttact gagattaatt gtaaatccag
    18541 ttataacttt atctacttgc ccactttcat ttcttttttc ttctacttta tatgaaatat
    18601 tattggcaat tttttcttta ctccaaggag tccagctccc attcattgga atttctacaa
    18661 ctctaaaatc atgcccagct tctcctgaag tagttaatct catgccttct ggtatattat
    18721 ctgttataac aacattactt attggtgatt tatcatcatt tattgaaatc tgatagttta
    18781 ttacttgacc aacttgagta atatttgttg ctcctgaatg tcctttagta agaagataat
    18841 catctcctct atcaaatata cttacagtag catcatcttc ttgtatcttc ttaccatcag
    18901 attcaatcct agctacattc ttatatttat taccatgttt tgcgtttttg tcactgattc
    18961 tagccttata cttaacagta tacgatttat ctatatttcc aaaattaact gaaatagaat
    19021 ttttactctg ttcaatatta aaatcactaa cttcttttcc atcacttgtt actacactta
    19081 cagaaccatt tatcaatgtc aatccttcag gaagctcatc atacaaaata atatctttta
    19141 actcttcctg cttttcattt acttgtatac tgtaatttat atctttattt ttaactggca
    19201 taaatacagt agttgatttt tttccttcaa caagcttttt aagtgttcct ttagcatctg
    19261 gaacatccgt tttatctcca ggttttattt ctacatctat atctccactt ccactttcta
    19321 tttttccagt agtttcaata tgtatttttg cattagtatc ttcatcaacc tttttaacat
    19381 atgcttccaa acctatttta ccttgcatta cactttggcc attaggtaat tctctaaatg
    19441 taagtgtata ctctccatta ctttctgttt tatcaaagca ttcacttgta taatctaaat
    19501 taactttatt gaatacatct ggcaccttaa aagttattga atctcctgct tgtattcctt
    19561 ttgatgttgg agtataagtt aaattaaatg atattttttt cattgattcc gtttctattg
    19621 tattgttatt aatttttaca acaaatccat agtcttcatt ttcttcatta agttcttctt
    19681 ctaaaacttt caataaattt tcatcttcta atagattatc ttcctctgga gttttttctg
    19741 tatttttatt gtctagtggt ttttcatctt ctaaagcttg ttctgacttt ttattatctg
    19801 ttgacttatc ttcttctggt atctgctctg gctttttatt atctattggc ttttctcctt
    19861 ctggagtttg ctctggcttt ttattatctg ttgacttatc ttcttctggg gtctgttctg
    19921 gttttttatt atctgttggt ttttcttcct ctggggtctg ttctggtttt ttattatctg
    19981 ttgccttatc tttctctgga gtctgttctg gctttttatt gtcgctttcc ttctcctctc
    20041 cagttgatga attagttgaa ccactttgaa tctgactatt ctctcctaat tctgaagcat
    20101 aagatacagt tggatatatt gtagacacca ccatacttaa aatcataatt aaactaatta
    20161 ataatgcctt tctatttcct ttcttcatgt aaatctcacc cctttaggtt acctataatt
    20221 tataatctaa tcttccatta atatcgtata taaagtaatt tttactaaaa aattatacaa
    20281 aaaaagctat gaaaataaat aaatttatat tcatagccgg ttgcaccact gactcagtat
    20341 atccataagg tgcccaataa tagaatatac ttcaacatct ctataatatt tcataaaaac
    20401 aattaaaatt gctttattta tattaaaaat acaattatat acttctatta tattatgcct
    20461 ctatactatt gtctatcctt tttacatttt ttaccttatg atgagattaa ttaataatta
    20521 aataaaatcc ttatctacaa caattacaca attatattca ggatttatct ccttaactaa
    20581 ttttgttata tcttctttta agtcatcttc tgacatgttt ttggaaaatt cggaaggatt
    20641 aactactata tcaaatatta aattcttctt gcttcctttt ccaactattc taaaatcatg
    20701 catagatttt acaagactat ttttcttaag tatttcttca agttcttttt tggctgacat
    20761 aacttcttta tcttcaagac atattgggtc catgtgaatt actagatata tatctaattt
    20821 ttcagatatt tctctctctg cagtatctat aatttcatgt atttccatta tatctatgtt
    20881 ggatggaatt tctgcatgta tagatgcaat acatctacca actccatagt tatgaataat
    20941 taaatcatgt gtacctatta tatatttata agataacaat agctctataa tagaatttac
    21001 catttcttca tctggtgctt cccctaaaag aggatttata gtatctttta caagagagaa
    21061 accagcataa agtatcacta atgatactac gataccaaca tatccatcta taggaaaatt
    21121 agtaaatcta gctacaataa atgatataac tacacagctt gaagtaaata catctcctaa
    21181 agcatcaagt gaaactgctt ttaaggctga agaatcaatt ttatttccca taaatttatt
    21241 aaaacgactc aaccagattt taatcattat agatgcaatt aaaagtataa gtggtattac
    21301 ttcaaatttt acaggaattg gatttactat cctttctata gatgacttta caaattgaag
    21361 tccaaccaac atcaccataa atgctactat taatgctgat aaatactcta ttcttccatg
    21421 accaaaaggg tgttctttat cagctggctt acttgctaat ttaaaaccta tcatagttat
    21481 tgctgaagat gccatatcag aaagattatt aaatgcatca gccattatag ctatactaga
    21541 tgtgagcatt cctatgaata cctttatcac aaataaaagt aaattggata atatacctac
    21601 tacaccagct acataaccat acttatttct aacatctgta ttttgaacgt tttcgctatc
    21661 tctgataaat gttttgacta ggattttgga aaacatgaaa cttacctccc tgccctttat
    21721 tttattaaca aattttataa tctatatctt tatttatgat aacataattt atatataaaa
    21781 ttcttaaaaa tcactctact tcagtaaaat attattctat atatctattt agatacgaat
    21841 tttatattta gtaaagtttt tatggcttag tgtctgttta atagattcta tataagtttt
    21901 tacaaagatt atttattatg ataatatcat ttttcccaag tattacttag aatatgattt
    21961 ttttgttttg tctgtacata ttttaaattt atactatatt tatctatatt ttataacctt
    22021 atgatacact ataactataa gtttttaaat ggaaaggaat gaaataaatc atgtttttta
    22081 tcagttatat ttacacaaaa tcacctattg aatttgatac tcctttgcaa aaagaggcat
    22141 acaaaatatt acaaaaatta gatattgact ttgaatgtgt tgacacagat gaagcaatta
    22201 caatggaaga ttgtgtttag attaataaaa aattaaatat gaaaatggta atataaaaca
    22261 acattaaaca aacaaaaacc gcaaagttac aatatatagt actttgcggt ttcataggaa
    22321 ttaattttta ctattaaagt tcatagttct actatttttc attgttctaa gaattagctt
    22381 tacaaattca tttgacaata ggatagtgga tgcaaatagt attatcttta tccacatatc
    22441 aaagtccaaa gaaacagaat taaagaaact ctggaacact tgtgtaaata ttatttgaat
    22501 tatccctgta actactatta tttgcaatgc cagtttattt ttaaagaaat ttggtattgt
    22561 actatttaaa ccaaactctc tacaatttaa tgcattaaaa agggcactga atgcaaataa
    22621 agaaaatata acagtacctt gttcttctga tgtagctccc aatatattaa aagctgattg
    22681 agtaaataca attgtaatta taagaatagc atttataata atatttacaa acatagacct
    22741 agctattatt ccagaatgtc tatttattgg ttttcttttc aatacataat ctctaacagg
    22801 ttctaatcct aatgctaggg ctggaggacc atccattatt atatttaccc ataataattg
    22861 tattgtagta aatggcatat ccttaccagt aagttgagat attatagcaa ttataaatgc
    22921 tactatattt acagtaagct gaaactgtat aaatctttga aagttctcat atatacctct
    22981 tccccatttt attccctcta ctatagtact aaagctatca tcagtcaata ttatatctgc
    23041 tgcattttta gatacttctg ttcctgaaat acccattgca attccaacat ctgcttgact
    23101 aagtgctggg gcatcattta taccatcacc tgttacagct acaacttcgc cacttttttg
    23161 aagtgctgaa actattctca tttttgtatc tggtttactt cttgcaacaa tagatattcc
    23221 tttgatttct tctctcaatt ctttatctgt caatacatct atatatgttg cttcaactgc
    23281 ctttttccca tcatttaata atcctaattc atttccaata gctgttgctg tatttatatt
    23341 atctcctgta agcattttta catctactcc tgcattaaat gctttatcaa tggaatcttt
    23401 gaccccttct ctcaatgggt ccactattcc tacaaatcca ctaaaaacta aattatcttc
    23461 cttcatataa cttcttgaat ttcctattaa attcaattca ccttctgaag tcattgcaac
    23521 ttctgtttta gaattactca tttttttata agcaaatcct aatgttctca tagatttaat
    23581 ttgtaacttc ttaatctcat ctaaaatact tttttctact tttggagtta ttggaacaat
    23641 attttttcct tgttgtacat aagaacattt ttttaataat acttctggag caccttttga
    23701 aagtaatata tctgaatcct cttgacgaat taatgttgac atcttctttt cttctgagct
    23761 aaatggtatt tgtgatatca aatacgtttg ttttctcatt tcattatagt tcttatcatt
    23821 atgatataaa agtaatgcac actctgttgc gcttcctata tatttaaatg aattatcttc
    23881 tttttctata tctgcagttg aatttactat acagttttgt tcaaaatagc tattactttg
    23941 atactctcct ccacttatat atttaccatc aacatatgct acctctacca tcattttgtt
    24001 ttgtgttaaa gtccctgttt tgtcggaaca tattacagaa acagaaccta tagtttcaca
    24061 ggcttctttc tttgttacta atgcatttat ctttgccatc ttttgcattg ttatagctaa
    24121 ggtaatattt atcatagttg gcaaaccttc tggaacagtt gcaactatta atgcaataca
    24181 aaccatatat gcatttttag caggttctaa tgattgtaag aaagataata cactgcttgt
    24241 atctaaaatt aacatacctt ttctctgcat ctgtataatc ataaacatac ataataaagt
    24301 agctattacg cctgatattc ccgctatttt agcacccaac ttccctaatt taatttgaag
    24361 tggagtttgc tggtcttcat ttgataaatt ctgtgctatc ttacccatct ctgtcttatc
    24421 accagtatga gtgacaacca ttatgcccct accatatgct acaagagtcc ctccaaatac
    24481 catattgact tgctttgcag gtattgcatc ctgttcgatt atttctgttt tagaataaac
    24541 tacttccata tctaatacag catcagcatt cttagctaca tcatcagatt ctcctgttag
    24601 catatcttct ctaagtttca aatttataga ctgtataagt cttccatctg ctggtatcat
    24661 atccccagtt tctatatata caatatctcc tggtactaaa tcattttttg atattttaat
    24721 tatcttccca tttctaagca cctttacttc tatattttct gttaattttg acagggcatg
    24781 agctgctttt ttagattttc cttcagttat tatgcctata gaaattccaa taaaaatagc
    24841 ccctaaaata cctaatgcgt catgtaattc tcccacaaaa gaacttatta ctgctgctcc
    24901 tatgagaatt aatatcatag gttctgtcaa actttctccc aactcgtccc aaaatgttct
    24961 accttctttt atagtaaatt catttaatcc atatctaagc ttcctttctt ctacctcatt
    25021 atcatcaagt ccaatttctg gatttgtttt taagtatttt aatacttctt tggtaggttt
    25081 attataatac ctcataactt tatccccctt gttaattatc tataaggaat tatatgctta
    25141 tgatagcttg tatattactt ttcaaaaaaa ataaggtgtt gattaatcaa caccttatag
    25201 tggttatata ttattaaatt ttatgttaca ggctttataa attgtgagct atacaaatca
    25261 aaatatttaa tcttcaaaat acccttctgg atataatgga aacttagata taagttcttg
    25321 tgcctctgct ttacattcat ctaatacttc tttattatca atattttctg ctaccttatt
    25381 cattatccct gctatctgta taacttcttt ttctttcaga cctctttgag atattgatgt
    25441 aagacctata cgaacaccac tagttacaaa tggtgattgt gggtcgtcag gtataacatt
    25501 tttattaact gttattccta catattcaag agctttttca aattgttttc ctgtaagatt
    25561 cttacttctt aagtcaacca ttactatatg attatcagta cctccactta caatactaaa
    25621 accatgtgaa atcagttcag aagctagagt ctgagcattt actacaactt gctccattat
    25681 ctttttaaat ttatctgtac ttaaatataa tagtgaaaag gcttttgctg caactgtatt
    25741 aagatgtata gaccctaatg ttcctggaaa aacaccttta tcgagtttct ttgcatgttc
    25801 tgctttacaa agtacaatac cacttcttgg tccacagaaa gtctttgtcg tagatgaaga
    25861 aacaaaatct gcataaggta caggactagg tattactttc gctgcgacta atcctgcaac
    25921 atgtgccata tctaccataa agtaagctcc tacttctttt gcaacctttg atattctttc
    25981 ataatctatt aatcttggat atgaacttgc ccctgcaatt ataagttttg gtctacattc
    26041 ttttgccttt gcttcaagag catcataatc tatacgtcca gtatttgggt ctaccccata
    26101 aaattcatat ttataaatgt catgtaaaaa attaactgcg ctaccatgtg tcaagtgtcc
    26161 accttggtca agacgcatac ttaaaacagt gtcatttggt tttaatattg atgaatatac
    26221 acaatagtta gctgtagaac ctgaatacgg ttgtacgttt acatgttctg ctccaaatac
    26281 ttcttttgca cgttttattg ctagggtttc aagtttatct gcttcttcag aacctgcttg
    26341 aaaacgagcc cctggatagc cttcttctgt tttatttgta aatacacatc cacttaattc
    26401 aagtacttct gttggtgctg tactttcaga agctatcatc tcaatattgt gttcttgtct
    26461 aaccaactca tcagctacaa tcttgtataa ctctgggtct gaaatttttg ttgtttttaa
    26521 cataataatc ctcctcttaa ttaaagaata tattttatta gtttaatctt cattgataaa
    26581 tttattataa cggtctataa aacgtaagat attcctccac caggtcctaa tggtatgcca
    26641 atacccatga atagtaacaa tattattatt tctattaatg taaataccac tgagtatgga
    26701 atcatattcg atatgacagt acctatacct atatctttat catatcttct ggcaaaggcc
    26761 aatataactg gaaaatacgg aaaaagtgga cttattggat ttgttgaagc atctccaata
    26821 cgataagcta tttgagttaa tgctggatta aagtctagta gcataaacat gggaacaaat
    26881 ataggtgcta aaatcgccca ttttgcagaa gcacttccta caaataaatt gataaatcca
    26941 gatagcaata taaatcctat tatcaatcca tagctaggta ttcccgactc tgctagtaat
    27001 tttgctcctg ttattgataa aattgttcct aagttactaa gattaaataa attaataaac
    27061 tgggatgcta taaatgctag gactatatat ccacccatat cactcatact actacccatc
    27121 atagatacta aatctttgtc actttttatc ttcttactta cttttccata cactaatcct
    27181 ggagttaaaa atataatagt gattatagga actatacctt tcattaaagg tgcattacta
    27241 gatagtatag aaccagtctc tggatctttc ataaatgcat tgtcacctat acataaggca
    27301 actatcacta ctactacaac taacaatgat aataaagcgt attttatacc cttattttca
    27361 agttcactca attcctgtat atccttatca tttgttgaat cattatattt tgtaaaacga
    27421 ggagctataa atttttcagt tacaaatgct cctgcaaaag ttaaaactat cgctgatata
    27481 aaagcaaaat aaagattcat tgctggattg ccttggtaag atgggtccat catttgagct
    27541 gctggtattg taaatccagc taacaataca tctgtagtac taagcataac atttgctgca
    27601 aatccccctg caacacctgc aaaagctgca aacataccta caagaggatg tcttccaatt
    27661 cctaaaaaca caagtgctgc aagtggtggt aatattataa atcctacatc tgctgctgta
    27721 tgtgacaaca tacctgcaaa tacgattgct acagttacaa gtttttgtgg aactttactt
    27781 ataccctttt tcattaacac ttccataaat ccagatttct cagcaacacc tgctccaagc
    27841 attgtaacca gtactaatcc taaaggagca aatgactgaa agtttgtaac tatgctacct
    27901 aaatatgatt gaatctgctc tttctctagt agatttacta cctttacaac ttcttttgta
    27961 gaaggatgta tttcttctac tcctaaatta gatataactg ctgacaatat tacaacaatt
    28021 atacatagta ataaaaatat ggttactggg tctggaagtt tatttcctgc tctctctaca
    28081 acatttagtg ccctcatcaa taatccacta tctttgtttt tttcctgtga attaattttc
    28141 ctcaaactaa taccccctca taaactttat ctccagaatg ctctatctta aattttattt
    28201 tttaatttat ccatataatt tataacagac tgacatagta catttgtacc taccaatata
    28261 gaatttttat cagctttaaa tttaggatta tgccactgat atatacaatc ttgttctttg
    28321 ttgcctaccc ctaaccagta aaaaaatcct ggggcttttt ccatgaatat agaaaaatct
    28381 tcccctcctg tagaaggtat tggatcaact atagcatctt ctgaatacaa atcacacaca
    28441 gacttacaag ctattgcgta catctcttta ctatttataa cagctggtaa atcaaataaa
    28501 taatctagct ttgcagttgc ttgatatcca cgagctatct cacaaactat attttctaaa
    28561 ttttctgata ttttctttcg tacttcatta ctaaagcttc tacatgtgcc tgtcatatct
    28621 accttttctg aaattacatt atttgcagtc cctccattta tagtacctat tgatataaca
    28681 gctgattcta atggacttat gtttctgctt actatggttt gaatacccat tataattgca
    28741 cttgatgcta ctatagggtc tattgttctg tttggtatac ctccgtgacc tccaaatcca
    28801 tttacttcta tcttaatagt gtctactgct gccattaacc cacctaactt aacacctatt
    28861 tttccacaag gtatatctgg atgattatga agtccaaata tagcatcaac ttttggattt
    28921 tccattactc ctcgttccac taacatcttc gcacctttat tgacttcttc ggctggttga
    28981 aatataaact ttacatttcc atgaagacta tctctaatat gagacaatac atatgcacat
    29041 cctacaaggc aagacgtgtg tatatcatgc ccacatgcat gcattatacc atcatatctg
    29101 gatttatact taatatcaac ctcttcatat attggcaacg catcaatatc tcctcttagt
    29161 gctactgtgg gaccatcata cttccctttt aataatccaa caacgcctgt ttccattcct
    29221 aaatctaata tttctatgtc taaactcact aataaatctt ttatagcttt tgtagtccta
    29281 aattctttaa aacttaattc ggggtgcata tgaatatctg taaaaaaatc taaaatatct
    29341 tctttatact tttctgagat taaaataagt tcatgcatac taacacctct ccaaaatttt
    29401 attttttta
//
LOCUS       DAMJWU010000040        29107 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMJWU010000040 DAMJWU010000000
VERSION     DAMJWU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.040"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            174..2816
                     /gene="adhE"
                     /locus_tag="PC460_001956"
                     /gene_synonym="adhC"
     CDS             174..2816
                     /gene="adhE"
                     /locus_tag="PC460_001956"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2600842.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3176..3517)
                     /locus_tag="PC460_001957"
     CDS             complement(3176..3517)
                     /locus_tag="PC460_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600843.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3544..4215)
                     /locus_tag="PC460_001958"
     CDS             complement(3544..4215)
                     /locus_tag="PC460_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600844.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4227..6803)
                     /locus_tag="PC460_001959"
     CDS             complement(4227..6803)
                     /locus_tag="PC460_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2600845.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVILANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7011..7946
                     /locus_tag="PC460_001960"
     CDS             7011..7946
                     /locus_tag="PC460_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2600846.1"
                     /translation="MANNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRVHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            7965..8702
                     /locus_tag="PC460_001961"
     CDS             7965..8702
                     /locus_tag="PC460_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600847.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(8888..13945)
                     /locus_tag="PC460_001962"
     CDS             complement(8888..13945)
                     /locus_tag="PC460_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF2600848.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(13970..14389)
                     /locus_tag="PC460_001963"
     CDS             complement(13970..14389)
                     /locus_tag="PC460_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600849.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14623..14874)
                     /locus_tag="PC460_001964"
     CDS             complement(14623..14874)
                     /locus_tag="PC460_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600850.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            14963..15694
                     /locus_tag="PC460_001965"
     CDS             14963..15694
                     /locus_tag="PC460_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF2600851.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15753..16664
                     /locus_tag="PC460_001966"
     CDS             15753..16664
                     /locus_tag="PC460_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2600852.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKFIEAGKFELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16715..17527
                     /locus_tag="PC460_001967"
     CDS             16715..17527
                     /locus_tag="PC460_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF2600853.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17636..18328
                     /locus_tag="PC460_001968"
     CDS             17636..18328
                     /locus_tag="PC460_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2600854.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18415..19356
                     /locus_tag="PC460_001969"
     CDS             18415..19356
                     /locus_tag="PC460_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600855.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLNQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19393..20550
                     /locus_tag="PC460_001970"
     CDS             19393..20550
                     /locus_tag="PC460_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2600856.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20702..21262
                     /locus_tag="PC460_001971"
     CDS             20702..21262
                     /locus_tag="PC460_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2600857.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21290..21781
                     /locus_tag="PC460_001972"
     CDS             21290..21781
                     /locus_tag="PC460_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600858.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            22010..22561
                     /locus_tag="PC460_001973"
     CDS             22010..22561
                     /locus_tag="PC460_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2600859.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22647..23579
                     /locus_tag="PC460_001974"
     CDS             22647..23579
                     /locus_tag="PC460_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF2600860.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23634..24497
                     /locus_tag="PC460_001975"
     CDS             23634..24497
                     /locus_tag="PC460_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2600861.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24744..26606
                     /locus_tag="PC460_001976"
     CDS             24744..26606
                     /locus_tag="PC460_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2600862.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26609..28075
                     /locus_tag="PC460_001977"
     CDS             26609..28075
                     /locus_tag="PC460_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2600863.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQRKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            28099..28935
                     /locus_tag="PC460_001978"
     CDS             28099..28935
                     /locus_tag="PC460_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2600864.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10757 a         3503 c         4665 g        10182 t
ORIGIN      
        1 ttgacaaaat attcagaaaa aaacttcttg acctaagaga gtaaaataaa taaaatctaa
       61 ggtatagaga tgaaatctat aaaattattt taagtataag ttaaaaatat agatgatgca
      121 tttaagatta ataaaaatta gagatgcata attattttag ggaggttttt agtatggaga
      181 aaaaagaaaa ggtagtagaa aaatcaaatg ttgaagtatg tagtcctgaa attgtcaaca
      241 gtgttgaaac attaagaatg agattagaag aaattagatt ggcacaaaaa gaatttgcaa
      301 catttacgca agaacaggta gataagattt tcttagctgc ttcaactgca gctaatcaac
      361 aaagaatacc tctagcaaaa atggcagtgg aagagactgg tatgggtatt gttgaagata
      421 aggttataaa aaaccatttt gcctcagaat atatctataa tgcttataaa gatactaaaa
      481 cttgtggtgt catagaaaaa gatgaagcat ttggttttac aagaattgct gagccagtag
      541 gtgttttatc agcagttata ccaacaacta atcctacatc aactgcaata ttcaaatccc
      601 taatagcact aaaaactaga aatggtataa ttttctctcc tcatccaaga gctaaaaact
      661 gtactataga agcagctaga gttgtacatg atgcagcagt taaggcagga gcaccaaaag
      721 gacttatagg ttgggtagat gttccttcga ttgaattaac taatgttgtc atggcagagg
      781 cagaccttat attagctact ggtggacctg gaatggttaa atctgcatac tcttcaggta
      841 aaccagctgt cggagttggg cctggaaatg tacctgcaat aatagatgag agtgcagata
      901 taaaaatggc tgtaagttca atcttagtat caaaatcatt cgataatggt atgatatgtg
      961 cttccgagca agctgtaata gtaccagaaa aaatatatga agaagttaaa aaagaattta
     1021 aatatcgtgg agcacatttc ctaaataaag aagaaactga aaaagtagga aaagtagtaa
     1081 taattgatgg ttcattaaat gccagaatag ttggacaacc agcacatgta atagccaaaa
     1141 tggcggatgt agaagttcca aaaacagcta gaataataat tggtgaagta gaatctgttg
     1201 aattaaatga accttttgca catgaaaaac tttctcctgt acttgcaatg tacaagtcta
     1261 aaagttttga agatgcagtt gctaaagcag aaaaattagt tgcagatgga ggctatggtc
     1321 atacatcttc attatatgca gactctataa atcatccaga gagagtagaa aagtttgtaa
     1381 atgctatgaa gacttgtaga gtattagtta atactccatc atcacaaggt ggtataggtg
     1441 atttatataa ctttaaattg gcaccgtctc ttactcttgg ttgtggttct tggggtggaa
     1501 actcagtatc tgaaaacgtt ggagtaaaac atttgcttaa tatcaagaca gtagctgaaa
     1561 ggagagagaa tatgctttgg ttcagagcac cagaaaaggt ttatttcaaa aaaggatgtt
     1621 taggtgtagc agcaagagaa tttaaggatg ttatggataa aaagaaagca tttatagtaa
     1681 cagattcttt cctatataat aatggatata ctaagaaatt aactgattta ttagatgaga
     1741 tgggaattaa gcatactaca ttctttgatg tagctccaga cccaacatta gcttgtgcaa
     1801 gagaaggagc taaagctatg gctgatttcc aaccagattt aataatagct gtaggtggag
     1861 gaagtgcaat ggatgctggt aagataatgt gggtaatgta tgagcatcca gaagtagatt
     1921 tccaagactt agctatgaga tttatggata taagaaaaag agtatatgta tttcctaaga
     1981 tgggtgaaaa agcgtatttt gctgctatcc caacttcagc aggaactggt tcagaagtaa
     2041 ctccatttgc agttataact gaccaagatt caggtgtaaa gtacccatta gcagattatg
     2101 aattaatgcc aaacatggct ataattgatg ctgatatgat gatggagatg ccaccaagat
     2161 taacagcagc atcaggtgtt gatgctttaa cacatgcatt agaagcatat gtatctatgt
     2221 taagaactga accagctgac ggtctagcac tacaagcagg taaaataata tttgaatatt
     2281 tacctcgtgc atataaaaat ggaaagaatg ataaagaagc aagagaaaaa atggcaatgg
     2341 catctactat ggcaggaatg tcttttgcaa atgccttctt aggaatatgt cactctttag
     2401 cacataaatt aggtgcattc caccatgttc aacacggtgt tgcaaatgca ttattaataa
     2461 atgaagtaat caagttcaat tgtgcagaag ctccaaataa aatgggtgca ttctcacaat
     2521 acagatatcc tgattgcata caaagatatg ctgaatttgc ttcttttgca ggtattaaag
     2581 gaagtacaga ccaagaaaaa gtagataatc ttataaaagc aatagatgaa ctaaaggcaa
     2641 aagtaggtct tcctaagact ataaaagagg ctggagtaga agaatctaaa ttcttagaaa
     2701 gattggatgc tatggttgaa caagcttttg atgaccaatg tacaggtgct aatcctagat
     2761 acccattgat gagtgaatta aaagagatat atctaaaagt ttattatggt aaataatatc
     2821 tattgtgcaa cataggttct attgggtttg tatataaatt attagatttg aatataaata
     2881 gagttttatt agagacacgt agaagtaact ataaataata gattataact taaattctaa
     2941 atggaaatga taaaaagtca gtaatttaat ggtaatttcg aatatgtgta tttaatcaat
     3001 taactttaaa attagtcgga aaattttttt attacatata attttaaagc ataatatata
     3061 aaaaacttta tgaaaaaatt ataaaaatat tcagaaaatt ctattgacta atttatttat
     3121 catgatataa taagtttaga aaagaaaaaa attggggcct attaaggccc ccttactatt
     3181 ttttgataga tttatttaaa attactttct cattttgatt ttttatattt attatagagt
     3241 cagtataatc cacttcaaag ttggcaaaag cagagaataa atctatatct cttaacaaat
     3301 gaattgtcat acattgattg ttcttattta gccttagaat caatgctctt acaaatggaa
     3361 attgtattct agaattaaat aagttagaac catcagtatc tatatctaag ctacgataat
     3421 taatatctat aaatatatct acatcatcat tttcttctct tattattctg tagtcaaaat
     3481 ccaagtttaa atttaaatta cctaaaatca ataacattta aatcctcttt tctataaata
     3541 gttttagagt gttaatttaa gctctccatc tattatagat ataattctat ctgcttttct
     3601 agcaatattt tcattatgtg ttattattat aagggtttgg ttatattttt ttatagaact
     3661 ttttagtaaa tccatgactt cttctgtagt tttactgtcc aaatttcctg ttggttcatc
     3721 tgctaggatt atacttggtt ttgcagataa agcacgtgca atagcaactc tttgctgttg
     3781 accaccagat aattggttgg gaagatgatt tttcctttca ttaagaccca ataaatttat
     3841 aaggtcatca atatattctt tatctatttt tttattgtct aactttagtg gtaattctat
     3901 attttccttt acatttaata caggaattaa gttatactgt tgaaagacaa atcctacctt
     3961 ttgtcttcta tatcttgcta attctttatc tgaaagacaa catatatctt taccatctac
     4021 ctttatagta ccattacttg gtctttcaag accacctact aaatgtagaa atgtactttt
     4081 cccacttcca cttggtccaa ttatagcaac aaattcgcct ttatttattt ttaaatttgc
     4141 attctttagt gcatgtacag atgtttcctt tttcccataa atcttaccta aattaatagt
     4201 ttccaatata ggcataatta tccctcctat tctgtaatgt ttatagcttc aactatacta
     4261 attttattta tctttcttga tggaatatat gtagctagaa taccaacaat tatatttgct
     4321 aaaataacta ggatatatat tttgtaatct attgaaaatc ccaatccata tgatacaaag
     4381 ccctgcatat aatacatttt aaattgtatt ataattcctg acacgatagt aattatactt
     4441 gagagtacac catataatat tccttcataa agtatcatat ttttaaagcc tctctcagag
     4501 ataccaatag ctcttaacat tccaaattca ctagttcttg aagttaagtt ataacttatg
     4561 ttgtttatta tgttaagtac actgattatg aacattattg ctactatacc ataactataa
     4621 atcatagttc tagatgtcat cttatcagaa ttttcttttt ctaccatcat atctactgtt
     4681 gtagttccag gaacttcact tccaatttta cctaaaaggt tatttatttt tttcttgttt
     4741 gcattttcat taaggtctgc ataaactaag ttatacttat ctacaccagt taattttttt
     4801 aggtattcgc tactcgttat tacatctaca ccatcatctc cagagtacat accattatct
     4861 gcaaaaggat aacttataat agctccaact ttaaattcat attctaagta ttcataatta
     4921 tcttttagac tttcataaag ccctgtatcc atcccaccct ttggatattt gacttttata
     4981 gtatcaccta cttttatatc tacagctggt gttccttcac caggagtaaa agtctttgtg
     5041 tatttctgtg ttttagaaat ttctggtaca tatacaatag ctaaattttc ttttttcatt
     5101 tttttcaagt ttatatttcc acttactaag tatttttcta atgactttat catctcatca
     5161 ttataacctt ttagcttttg ctttagtgta tatttgccag tagtcttatt ttttatgaac
     5221 agaggattta gcttataata aggagttgaa tttatagctt cgtagtaatc taatctattt
     5281 attttattta cctctattct tgtgtttaag atactagcag ttttgacttt gtcaattcca
     5341 tctatatttt ttattttatc tatctgatta tcattaaacc cttttatatc tttaaaagaa
     5401 gtattgttac caatttcatt gagataaaca tttgatagaa taaaatctgc attcatatat
     5461 gtttgttctt gagtagattt gttttgcaca tctaaaaaag aatatttata attgtctttt
     5521 atgacgatta tacctcctat actcatagat aatacaatta taatgaaccc ctttttattt
     5581 ctaaagatgt ttttcgcaga tatagatttt gttactgaca tatttaaagt taattttgat
     5641 attaaataat taactttggt tacattttta tcagctccaa aagtttttct gatagcatca
     5701 ataggagaaa ttcttcttat ttttaagtat gtaaagaaac ttataattag tattattagt
     5761 attgtacatg caattgataa gagaattatt ttatctggaa taacaaaagg tgtaacattt
     5821 atatttcctt caaattgaat atttcctaca gacctattaa atatttgtgc acctcccata
     5881 cctatacata tacctatagg cattgctatt agagcaagta tgaaaagttc ataaagcata
     5941 agtttgaaaa tcctaaagtt ggtggaacca atagctctta agacgccata ttcctgtact
     6001 ctttggtata ttgatataac atagatacta tatattacaa taccagcaaa tagactcata
     6061 gcaattgcaa cgtatttact tgaattatct aaagtaccgt ttttcatagt agattctata
     6121 agcattgagt ttcttcctat acttttctca tctaacatag cattttttat tatattgtct
     6181 atgtttgtat ttatatcact gccttcatta aattcaatat aagttgtaaa atcattcagt
     6241 tctgattcat tgagattaga aaaaatttca cattgtccct tttttttctc tataaatcta
     6301 tcttcaagga ttccaacaac tttaaaagtt tctggatttt ctttttgata taatttaaaa
     6361 gtaaggtttt gatttatctc tggttttaag cccatactat ttaaaatcca tttctcgact
     6421 accacctcat tgctagcttt tggaaatcga ccttttataa gtttagaatg actagtcaaa
     6481 taatttttat ttgcatatag tatgtttata ggtaattttt cattaacatc tgtagacgca
     6541 tagtaagatg ttatacccag atttttaata tctttgctag attttatgga ctttaattga
     6601 tttttcttta tatttttaaa gtatacatgg tcagttccaa gctctctttt caatctatct
     6661 acatctgctt gctgagcact tcttgagagg gtaccaatac caacaacgag tgatgtagca
     6721 agaattatac tcacaacaat tgctaaagac ctagctttat aactttttat ataccttagt
     6781 actattttca aattatcttt catatttacc acttcctttc ttaactaaat caattatata
     6841 tttatctaaa atatatagca aagtaagtat tttcaagttt taagtagtta attgtacaaa
     6901 ttcatctgta tttttacaag tttaattaat ctatatgtaa attttaactt tttacataat
     6961 ttttttaaaa tgatataatc cctatataaa ttgtagaggg gagggaaatc atggctaaca
     7021 attttgtatt tcctatagta atactaactt tgtttatatt tagcaatctt ataatgaata
     7081 agtataaatt ttacaaagaa ttagacatat taaaaagagg catattaaca ttttttatat
     7141 cgtgttttgc attgatttta tttatggagg gcactaaata tatacaatta aggttaggat
     7201 atagtttttt acttgaagga gtattaaata ttatactttt tttggtttta attattttaa
     7261 gtttttatac aggctttaaa gttataagat tgataaataa taataatgaa caggaatata
     7321 aactacaaaa actagatagt aaaataaatc ataaggataa tataattatc aaattaagaa
     7381 gtcaaaaaca tgactatttg aaacatttgc aagtggtgta ttctctatta aatactgggc
     7441 tagatgaaga tgccaaagaa tatataatga atatatcaaa tagttttaaa tcaaaaaatt
     7501 caaggtatgg aaaagtatct tatttagatg ctatagcttc attaaaatac gaggaatgtc
     7561 ttgaaaagga tatatctttt gatgtctaca ttcaagattt tatggataat ttagagattg
     7621 aacctaatga tatgagttca gtactattaa acataataga taatgcaata gagtctttgt
     7681 ctactataaa aaaagaaaag aagtatataa gagtacatgc atatgaagat gaatttcagt
     7741 atattatagc tataaaaaat aatggtccta aaatacataa tgttgaaagt atattttgga
     7801 atggatttag tactaaagaa ggacaagata ggggatatgg gctacattta gttaaaaata
     7861 tattagataa gtatgattat agtatacttg taaatagtga tgatttttta actgaatttg
     7921 taatcttaat cccaaaatat tattagttag gaggagaaaa gtctatgata aaagttttga
     7981 tttgtgatga tgataagatt gtaagagatg atatagcaaa tatagctaaa gagtctggat
     8041 atgtagatta tgttaaaaaa gttaaggatg gtatagaggc aattgaattt ataaaaaatg
     8101 agaaaataga tttattgatt attgacattg atatgccata taaaaatggg attgattctg
     8161 ctgaagaaat tctttctata gataaggata tacatatagt atttgtgacg ggtttcgctg
     8221 attatagtct tgaaagcttt aaagtgcatc ctatagattt tgtagttaaa ccatttaaaa
     8281 aagaaaaaat acttgattca attaacatag caatagacca tattaactcc cataagattg
     8341 ctaataataa ttttatagaa gacacccttt ttgtatataa aataaggaag cagatacata
     8401 tgattaattt tgatgatata gttatgtttg agaaaaattc aagagcaatt aatctacaca
     8461 ctaaagatga agatataata aagttttatg agaactttga tgatttaaaa aaacgcctac
     8521 ctcctaattt ctttatgacc catagacaat acatagtaaa tctaagattt atacataagg
     8581 ttatacctat aaataaatct tcattagaga taaggtttat aaattttcaa gaaaatgcca
     8641 tattaaacaa atctctagaa aaagattttt tatatagatt ttatagggtt aaaagattct
     8701 agtgaagata gaagaaaaat actttttgtg tgatataata agtaccataa agataatgta
     8761 taagagaagt ttatataaaa attatttgta attatgattt aatctatata aattacagta
     8821 tagtatatat tggactttct aatttttttt aagaaagtcc aatatatttt agggatagtt
     8881 tttatactta taaattactt aagatactag ggtctttaat cttactatct tcagcaagta
     8941 atagtatctt agacataatc tcagcagttt tagggtcttc atcagcgaat ggaaggaata
     9001 tcttacctct actttgagaa tgtataggaa gtatattcaa agcacccatc ccttttttat
     9061 gtacaatacc agaacccata tgcacagaat actcacctaa attacctgct ataagagcat
     9121 gagagccttc aaaattgaca ttgttaattt ttaataataa taaaagttct ttagcaattg
     9181 ctatacgcat ctctacagta gaatggctag tttcagggtc aacaccacca gcatgagcga
     9241 cacttacaac aaggtctata tcacgcataa cttctgagaa tagaattgga ggtatagaag
     9301 ctaattctaa acttttataa gtttttctat caaaaaattc tactgtttca atagtaggag
     9361 gctctatatc tgctggtgaa aaccaatctg caagtgcaaa catctttgcc actatatttt
     9421 ctttataaaa tactttctgt aatccctctt cataatcgac agtccaaagc tgatttctaa
     9481 gaagtgctac agttttcttt ggctgaattt ggtgaccagc atatctgcga gatatagttt
     9541 tttcagataa ttcatcactg tttattctat aatactcacg gaatacttgc ttaaatggtt
     9601 gaacaatttg cttactaaat aaatcatgtt ggaactcact ccacttacca gattcaaaca
     9661 aatgaactgg atgtgcaagt tttaatttat tttttgccgt caacttataa gtttcacctt
     9721 ctatattgca aataaagaac ttagacttat cattataaac atatccaata ttatcatcac
     9781 atacccatac tagattttta atcaaagggc agataactgg atttttatag atattggcaa
     9841 tttctttaac tgtaaatact atttcatttt tcatagcttg ttctaaactc tgttttgcac
     9901 gagaatactg actctttaaa tctttttgga cagatttaag ttcaagtaca tatggatgtt
     9961 tagcaatagc ttttggtata ctctttaaag acttgttgcc tttttgaact ttagtagatg
    10021 cttttccgtt ttcatcaaca tctatccaaa ttgaagtacc atcaatatct tttggtttaa
    10081 atagatgagc aacttcattt agtttttcag tctcaagagc ccacatcata cgatttacat
    10141 ctccaaatcc agcacttcta gctagatttt ctagcgctat agtacaagct tttgattcac
    10201 tttcacgacg ttgtgcacca aattgcttgc tttctttagc aaacttttga ataaattcat
    10261 aacggaaagt tgcttctttt aggtctcctt ttttcatagg aagtaaagag aaacttctca
    10321 atttatcttg atttctattt gtttcaattt ctttcatcaa catgtcttta tcaagcttac
    10381 caagtgtagc atcagcatag agttgtgcac gtctatgaga gttaccacca ctagttatat
    10441 atttagctga cttgtaaagt atattgaatc tttcttcccc aatagtttta tatgaatcta
    10501 aaaaccaatc tacatcaaat gcaccatcaa tgaaactacg tggtgaaata ggggagaata
    10561 gagcaacctc tgattctttt tgtgcagaga agccttcatt tatatgtgca tggaagtacc
    10621 aaattgtact tttcagaccc ttccagttta aatatttttc aattatgttt acccattgag
    10681 gggcatacat agcagattct atcaatctta actcagtaat gtcggtattt ttaagtaaac
    10741 tttttaattt ttttgagtca tcttctttag taggatagca atttttaagt agagaactta
    10801 aaacatcttt ttttgaataa ttgctaccat atgaatatcc tctctggaac gtttctttac
    10861 caagagcgac caaaatattt acaaaataat ttataccttc aaagtattgt atattattag
    10921 ctagatgaga cacctctgtt ggcatatcac ctctacgtga ttcaatttct ataattcggt
    10981 ttgtaacagt ttttaaatta tctttaagcc aagggtactt ttctaatgtg tcgccagccc
    11041 atctaggatt tgtcaatgtt cttattatat ttgatgcgtt gtatccactt atcatttctc
    11101 tattaacttg ctcttcagtc attaatccta attcatgtgc acgagcaaac acatttatgc
    11161 tagtatagaa aggaaactta tattctagca actgataatt tgaaaacgta gtgtagaaaa
    11221 acttagtaaa agaaatatca tcataaataa acgaatgtgc tttatcaagc caatagttta
    11281 aaataggggc atttaaagca gggcagtatt cattttgtct gtaatagttt ttagtaaatt
    11341 caactctttg ttttttacat tgttctatag tcaataagtt tctaagttta tagtatattt
    11401 ctaaacaata gttaaatatt tctttatggt caaactcatt tagtaaaact cttaaaagtt
    11461 ttcttgtaag tccattaaat ttaagttttt caagttttgt atatatttta ttttgttttt
    11521 tagagtcaag gacatttgaa tatatagatg aaaaccaact tttttctgtt tcattatatc
    11581 cacatgtact tatactataa aataaaatat caagtattaa tttgtaatct aagtcaagct
    11641 ttttactagt ttctctccaa agctctgcta atggataatt atcaagagta tcattagaaa
    11701 ctttatattt aataggagtc aagttataag tagaataacc taaaagtact ttgtctacat
    11761 tacctgacca tctttcaact tcatattcat aatctgcatt ttcagtaata agctcatcaa
    11821 atttttcata aacttttatt attttattta tatccgtatc gagaattttt tttgcttctt
    11881 tttttgttgc tacagaaaca gaatcctcac gagtagataa acttatattt gagataattt
    11941 tattaagctt tgatgagcta ttagaatcat ctttaaaatc aaattgttct ggaatttgaa
    12001 ctttgttttc agggttatat attccaaaac cattttcttt gcagtaatca ttttttgctt
    12061 tacctaacaa attatcaatt agtatttgaa cttgagatgt attcttttta gatttatcaa
    12121 tatcttctat aagtaaaaca agcttattat atagttcttg tctagcagta ttgtctttta
    12181 ataagtttac aatttcaaga cctccaaggc atttttgaga atttgaagat tttatcagtg
    12241 aagaagcaat attttccaaa tttgaatcat cttgttcaag taaaattttt attactgttt
    12301 gtctaataga gcttgtcttt aaagacaata gattggttat cttatctaat tctacatctg
    12361 ttagctcaag ctttctaagt agactcaata ccgtttctct attggaaaca ctcttatcac
    12421 ttaaacattc caataaaaat tcacgttgtc tatctgtctt tggttcattt acaaaattga
    12481 ctataagtga agctcttaaa tctgaactca taagatgaca attttccata agcacatcta
    12541 taaattgagc attcatatcg tatgctgtta aagtcatcat acatttaagt atttcatgag
    12601 aagaaatttc aatccaagtc catggaaata cacttcctga aaatgatttt gtctttgaat
    12661 ttaaaatttc taaagtatgt tttaaacttt caaattgttc ttttctagca tcaaagtctt
    12721 tgtcaatagg agtgtttgac aagtcaaaat tagtttctct gttttgccaa ctatagtagt
    12781 agccaacaga accttctaca tagaaatttg taattaccca tgctagtatc tctaaatcat
    12841 cacccttaat gtttttagat gctatagaat gtttaaaata tgtactttct gtatgcgata
    12901 aaaagtaaag agctacaagt tttttatatt tttcctgaca agaaagtaag ttttcaataa
    12961 gagagtaaga gtcatatatg ttgtaagttg cagtagccca taaggcaaaa taaatttcaa
    13021 gattatcatt gctattaatt gattctatac ggaacttttc atcagtcaat gatttatatg
    13081 aaagttctaa acatttacgt attattccag gtttttggtc tgaaaaacca agtcctgtcc
    13141 atgtatccaa ggctcttata acagaactaa atcgctctaa attgttgtcc aaaatagttt
    13201 ttaacatgta tattgatgca gaaattgtac cctcatctaa actctctaca atttgctgtc
    13261 taagtccttc ttgttgtttg gctgccaaaa gaagtttaga aagcatttca tagcatttat
    13321 cactatgact cttcatgata ccaattatca tttgtcttgt aagtagagca gtttgatttt
    13381 ctccaaatat tacttcttct aaagcactta ttactttttg atttcctttg tctatttcta
    13441 gtgctatttt atcactcatt atagagtcat agtacaagac cttatttgga agagtaagat
    13501 aatctataac tgaaaaatca taataaatta aatcaacata gtttgaaata aaccttataa
    13561 tacctctaaa ataatccaca agtttatttg agcgataccc tttacgataa tatcctcctg
    13621 aatatgtata atccatcttt cttttaatta attggcaaag ttcttctact tgttcagaag
    13681 taaatattaa ttctaatgca ttttttaaag gtccagaaag gagctcgtcc atatttttaa
    13741 tttcgtagta gtggcaattg tagtatttct ttatcatatt taagaatttg tcttcaggtt
    13801 ctctataata gtatgaacta ttttctatgc agtcccaaag agactttgct aagtcgtagt
    13861 actgttttga agaagattta gtaaaattat ttttaaaatt ttcaattatt ttaggatttt
    13921 tttctactct agtacgttct aacatttatg tacctccaaa ttatttattt taaaacattc
    13981 ttccagcaag caaggtaagt cttataaaag taaatatatc tccacttttc agatttatag
    14041 aggaatcgag tttttcaata acatcttcat ttataagcac tttgtagatt ccatcttcaa
    14101 atgcctgtaa cgctacctct attgcctttt ctacagattg tttattctca ttgtaaatag
    14161 agccaaatcc aacttttcca atatcagctt gagattcaat ttcttcatta gttaagaagt
    14221 ttattattga tttttctggg attttctcat tatattcaag aacattttta gatacaattt
    14281 cagagatagc ttcttttagg caattgatgt tgtcagatac atcaaaaggg gtaagttcta
    14341 gcataggttt gcgtttgcca acagccttaa ctctaaaata aatttgcata aaatcacctc
    14401 gcaaaatatt aattatgtat ttagaaattt aaaaatttat ctatttgtta tattatatca
    14461 ttttgtgaat tattaatgtg aattatggaa ttattttaca aaaaatagaa aaaaagctat
    14521 cttaaagtct aaaaaagcaa taaaagataa cttttaacta taattatata gaattataac
    14581 tgtacattta ttaaatttta attttagaat tatttttgat aactatcttt ttttatttat
    14641 aaattttgct cctaaatatt ttttgatatc ttcttcagga gaaaagtcat caataagtat
    14701 ttctttccca gtgtcttcaa atactacaag aacttcacag tcaccttgta agaaaaattt
    14761 tctaactgta tcttctttaa cttctctaac ttttattaca ttcttttttt ctttttcaaa
    14821 gtcttttgtg taataacttc ccatcatatt aattattttt gtatatctat tcataaaata
    14881 cctccatatg cgattaaatt taaaaaatat gttataataa ttgtttgtat taacttaata
    14941 attataacaa ggagtaataa taatgactag tataaaaaat aaagatattt taaattgtat
    15001 agattattcc attaaaaata agctctttga caaactaaat gatgtatata agtccttgcc
    15061 agtaggaaat tgttctggat gtggaaattg ttgtatggag tcagtaggta taaatttaat
    15121 tgagtttttg aatatatttt gttatcttga agatagagtt gatttaaaaa aaagctgtat
    15181 aagtaaaata ttagattatt attttgagga gtatacaaga aagaacccat gtccatttaa
    15241 agatactaat aatagatgtt taatatatga agttagacca ttaaattgta ggatgtttgg
    15301 acattggaaa aaagaggatt ataataaaaa tttagataat gttattgaga aaaataataa
    15361 ctatagagag ttaatgaaaa agcaatatgg atttgaaata aatgatgaag tagtaaatta
    15421 caggatagat tattgtgata gatttattcc aaataaagat tatttaagta aatctaagag
    15481 attaagcttt tttgatgagc ttatgatatt agactcaaat atttattcaa gtggaaatat
    15541 agatatagac tttagggata gaggtatagt tgaatatttt atagagtcat tactttatag
    15601 aaatttagca tataatgtga aaataaggat atctaaagaa tacaaaataa gaaaaagaac
    15661 tatagataga ataaaaagga ttgttcttgt atagttttat attagtaagt aataaattgt
    15721 atcataaata ttgtcaaaat gaggtgaaga aggtgaatct tacaaaagta aaaggaaata
    15781 ccttttttat aaaaggtggc acgaatacag gaatatatgt atttgaagat aacacagcac
    15841 ttatgataga cccagggtta tgtggttcaa gacctaggag agtaaataaa gtattagatg
    15901 aaaataatat acaggttaga tatattataa atactcatgg tcatgatgac cactatggag
    15961 cttgtaatca atttaaagaa tattataaag atgtgtgtat aatgtcatct gaatacgata
    16021 aattatatat agaaaatcca gaattgtttt caaagtatat tgttggggga aagagcaatg
    16081 tatttatgga taatatctta aaaaataaaa tgttggatga tataaaaata gataaattta
    16141 tagaagctgg aaaatttgaa ttaaacaatg aaatgtttga tataattgaa tttcagggtc
    16201 atacacctgg tagtatagct gttttgacga aggataatgt gatttttgtt ggcgatttat
    16261 tgataggaga tgaaatttta tcaaaatacg atttcttgtt tctatatgat atacaagaac
    16321 aaataaattc attagaaaaa ttaaaaaata tagattttga gtacttagtt ttagggcatg
    16381 gcaagcaagt tatatcgaaa gaagattctt ataaattaat agaaaaacat ttgaatgctt
    16441 taaaaaaata tttatatcaa ataagagagt tgcttgtaga accgaaatct ttagaaattt
    16501 tattaaaaaa cattataaat aacaataatt taagcaataa ttataaagag tatcattttt
    16561 ttaaatcttc gttagtatct gtcataagtt acttagttga tttagaagaa atagggtata
    16621 tattggaaaa tggagaattg ctttactata caaaaacaaa ataaattatg ataaaataaa
    16681 gaaggtcata aaaaacaaat aaagggtgag caatatggaa tacgaaaaat ggaatgagat
    16741 actagcacct tatgaacatg cagtagagga attaaaaata aaatttaaaa atataagaaa
    16801 agaatattta gcaaaaggtg aatattctcc catagagttt gtcacaggta gaacaaaaaa
    16861 aatatcttcc ataatatcta agttaaaaag gataaatgca aaagatatag aaactgaaat
    16921 tgatgatatt gctggtataa gaataatgtg ccagtttgtt gaggatatat atgctatagt
    16981 tgatttgata aaagtaagaa atgatatgac tataatagga gaaaaagatt atattacaaa
    17041 ttacaaagat agtggttata gaagctatca tgttataata aaatacccta taaactccat
    17101 tgcgggttca aaggaaatta tatgtgaaat acaaataaga actcttgcaa tgaatttctg
    17161 ggctacaata gaacattcat taaaatataa atatgaccat tacatacctg agactcttgc
    17221 tgaaaggctt agaagagcat ctgatgctgc ctttctttta gaccaagaaa tgagtgaaat
    17281 aagagaggac ataatgaaag ctcaggctat gtaccaaatg aagtctatag ctattagaga
    17341 tactctaaat agaatacaag agttatatga cttgggtgat acacataaag ctatgcagta
    17401 ccaaagaaaa cttgatagaa ctgaaacaga taaaaatata actgaaattt tggaattaaa
    17461 acaagaaata gatatattat tagagcaata taaggatact aaggtagatg ataaagtagt
    17521 tcagtaaaaa ctacttgtag aaaatttgct aaatattaat ggggctgcct cttaatggaa
    17581 aataatttct aaaaagagac agcctattta gtgtaattaa gaaaggaatg atattatgag
    17641 tttgtatgct ataggtgatt tacatttttc aacatcagtc aataagccaa tgaatatctt
    17701 tggaagtaat tgggatggtc atgaaaagaa aattatagat aattggaaag aagttgttaa
    17761 ggaagaagat atggtcttag tactaggaga tacatcttgg ggaattaatt tatccgaagc
    17821 taaaaaagat ttagatataa taagcaaact tccaggtcag aaaatactta taaagggaaa
    17881 tcatgattac tggtggacaa ccgttacaag tttaaataag ctatatgaag acatgcgttt
    17941 tattcagaca aatttttatg aatataaaga ttatgcaatt tgtggaggta ggggctggat
    18001 atgtcctaat gatgttaaat ttgatgagac tgatgaaaaa gtttataaaa gagaagaaca
    18061 tagacttaga ctatcattag aatctgctcg taaaagtggt cactcaaaaa taattgtgat
    18121 tactcattat cctcctacga atgataaatt ggaagaatct ttatttacaa agttatttga
    18181 agaatataat gttgaaaagg tgatttatgg tcacttgcat ggtaaagaat cttttaaaat
    18241 gggattgaag gggattcgaa atggagtaga atatacatta gcatcttgtg actatacaga
    18301 atttaattta ataaaagttc acgattaaaa aacataaatt ggtaaatact ataagataca
    18361 tgattaaaaa taaatatttg agggatttcg gttttgagaa aggagacacg acttatgagg
    18421 tggatattag tttttatatt tttatatctt attcctttag tagtgatatt cagaaattac
    18481 aagaatttta agaggtcttg tatctacagt agtatttatg tggtattagc tacaacaata
    18541 atgataagta atatttatat gagtggactt aataaaataa aagagtctat gtattatcac
    18601 aattatattt ctgatgagac atatgatgaa aagtatgtat caaattttaa ggaaaaagaa
    18661 agtactgatt taaaccagga taataaaaag aatgaagaaa tatcaaataa taaaaattta
    18721 aacaacaaac aagatagaaa tatacaaaat aaagaaagta atgaagaagg aagtaatgat
    18781 ataaaagaaa agaataattc tgatagtaat gtttcagtga ataataaatc agaaaaacaa
    18841 gaagataata caaaagaaaa taataaatat acaagtaaag aaaaaaatag tgaagatata
    18901 aaaaaaatag atatggaaag tataggaatt tttaagaaag aaatttataa tatagaaaga
    18961 gcagctttag ttccaatgag agaatgcata ccttacacta aggatatagc aaaaaatata
    19021 actaaattat ctagtataaa aaaagatgtt acaaatgcca gaaacaagtg taaagaagtt
    19081 gtaaaagaat atgaaaatat gcagattcca gaactttcta aggagaagta tacagaaaca
    19141 ttgagtaatg ctagagatga cgtaaagaaa gcatatgagc ttagagaaaa atctatggaa
    19201 agtgctttga aattaataaa tactaaaagc cctaaatata tagggaagat aacagaatat
    19261 cttgatttat cagataagca gatagaaagt tttaaaaata gattgaacga tttaaaagaa
    19321 gaaataaata attcagaaac aaataatatt aattaataag atttaaggat ataagttaaa
    19381 agggagatta tgatgaataa acagagtata ggaaaactaa atatgttttt tatggggatt
    19441 actcaaagaa accttgataa tagggatttt tttattgagt tagaggtgat ttttaaatct
    19501 ggaagaaaag agtttaaagg aattgcaata gaggaagata ataaatataa atttaatttt
    19561 aaaggaaaat cagatttata tacgtttaat gagttgctta agaatatttc taaagaggca
    19621 gaaaattatg atggattagt atttaaatat gttgagcgag gaactattgt tgtaattgaa
    19681 ggagacaata agaaggtaaa tgtaaaatat ttagataata aggaagaagt acctaaaata
    19741 gatgaattta cggcatctca aataaaaaac agagattatt atgtaaaagt aggacaagca
    19801 aatgcacttt taaaagaaat tggagtactt acaaaagatg gtaagataaa aaatgataaa
    19861 ataagaaaat acaatcagat agaccatttt gttgaattga tagatagtat tttaaaagaa
    19921 ataaaggata aagactgtat aactatcttg gattgtgctt gtggaaaatc ctacttaagc
    19981 tttgtcttaa atttttatat aaaagaagta ttgaagaaaa actgttattt tataggaata
    20041 gattattcag atgtagttat agaagcatca aaaaatatgg ctaaaaactt gggttataaa
    20101 aatatgtcat ttataaaaga ggatttgact aattatacac ctaataggga tgttgacctt
    20161 gtaataagtc tacatgcttg tgatacggct acagatatgg caataggtct aggaattaga
    20221 gcaaaatctg aagctatagt agtagtacca tgttgtcata aggagctatt agggcaatat
    20281 agatatgaag ctatggaacc aatattaaaa catggtgtat ttaaagctag atttgcagat
    20341 ttaataactg atggtttaag aacattgtta ttggagggca atggatatga tacttctgta
    20401 gtagaataca tatctccact tgatacacca aaaaatttaa tgataagagc gataaaaact
    20461 aaaacgaata atgacaaagc attaaaagaa tataaagaac tgaaatcaca atttggagtg
    20521 gaaccaacct tagaaaaact tatatattag ttaaaaatgt aaaatttacg aaaagcttgc
    20581 ttaaatatgc aagctttttg gtattatata ggtatagtca tatccagagt tttgatttat
    20641 aattaaagct gtgctataat atttgattaa tcaaatcaat aaatgttaca tggaggtaaa
    20701 aatgaacaaa ttgaatatat gtattgatat agatggaaca ataacaagcc cttatcattt
    20761 tcttccatac ctaaatgata tctttaataa aaatataact gaagcagagt gtataacaca
    20821 taattgggaa gaattgtatg gttcgggaat aagagatgtg tatgcggagt ttaataataa
    20881 gtatatacac tcatatgatg aagcaaagat tgtagagggt gctacagagg taattagcac
    20941 attatctaaa ggacataatt tatcttttgt aacggctaga catgagtgtt taactgatgt
    21001 aactaaaaat tggctatcga gacaaggatt ttcagatata gatgtttact tattaggaag
    21061 tgattataaa gttgaaaagg caagagaact gagttgtgac atatttatag aagataatcc
    21121 tttaaactca gtgcaactag ctgatgatgg tgttaaagta atacttctgg atactaacta
    21181 taataaagat gttaaacatc aaaatatagt tagagttagt aattggatag atattgaaaa
    21241 gattataaaa caaggtattt aaagatttgt agattgaaag ggggagcata tgatagattt
    21301 aaaaattgaa aatagaaaag gaaaaatcca tgtaaagagc agtgaaataa aagacatttt
    21361 agaattacgt ccagacttcg aatatgtaca agatatatca aatactataa atcaagaaaa
    21421 tatattggta tttgattgcc agttgacagg aaattctttt gatatggatg atttagatat
    21481 agaagaaata ttggaagagt taggtgaaga aatagatgag tcttatttca gtgttttatt
    21541 tgaagatgta agagcgtatt taaaagatgc tactgatgag atagaagcag acttacagga
    21601 taattactta tttgataatt tgagatgtta ttttgatata tacaatataa atcaagaatt
    21661 tacagatttt aagtttgtat ttttagtttc ttttaaagat ataaaaattt cttcattagc
    21721 aaacctagct aagattgttt ctaagagaca gcttataggt gcatctaagt tttatagtta
    21781 aaatgaaaga ttattataat aaaagctagt aaccttgtaa aaatataggt tactagtttt
    21841 ttatttattc attttcacaa aatttcagaa aactatttac attctaaaaa aagtaatgta
    21901 tattaaacat aatgtacatt ataatggata aaaagtttct attaaattac taagtttatt
    21961 taataatcaa aataatttat cttatattag tatgtattag ggggtattta tgcaaagttt
    22021 aaatgaaata attgctgaaa atttaaaaaa gataaggaaa gaaaaacatc ttagtttaga
    22081 caagattgca caacttagtg gtgtaagtaa gagtatgcta tctcaaattg aaaagtgtga
    22141 ggtaaatcct actatatcta ctttgaagaa aataactaat gggctaaaaa tctcatttac
    22201 atcattgatg gagaggcagg aaagtgacat agaattgatt caaaaaagtg atatagacca
    22261 ttttattgaa gataatggaa agtatataag ttatccaata tttccatttg actccaaaag
    22321 aagatttgag atatttatga ttgaaataga agaaggtgga aatttagatt caaatgctga
    22381 acttccagga acacaagagt ttataactgt ttttagtggc gaagtaacca ttaaaataaa
    22441 tggagaagat tacattgtat ctagtggaaa ttctataaga tttaaagctg atgtatcaca
    22501 tatatataga aactcaggca aaggtatagc taaaatgagt atggtagtat attatatata
    22561 aataatttaa tggaattaga gtgatattta gatgatttta ggtatgaaga tatattatat
    22621 ttaagttata ggagtgtgat ttatggatga ataataaaaa agcagttttt ttcttttgca
    22681 gtgatttaaa gaaagaccca gtagccagta atgtgataaa atgttgtgaa gaaataatgg
    22741 atttaaatca aactgatatt gttattgatg ataattttgt attagagttt aaggataaaa
    22801 acaataactt atttcatttt gtaaaaacaa aagatgtaat aagtcataat tataaatatt
    22861 atcttccaat tttaaataga cattttagag attatgattt tgcaggagtt gtaaattggc
    22921 atgagggaga aaatgctcca gaacatatcc taacagctca tacaattgga gatgttccaa
    22981 caggagaatt tggaaattca aatccaagat actttaaaaa tctaatcaat gctattgagg
    23041 atataaaaaa tgaaaatcta cttgatgaat ttactacatt aacagaagca actcactggt
    23101 ctggaacaac atatggtgaa gaatctaaat taattacaga atattctgta cctatgttgg
    23161 atattgagat aggaagtagt agtgatagtt ttaataatag tatagcaata caggtattgg
    23221 caaaatcatt aattagagta tttgattgtg atgaaccttt gaagactttg ttatgtgttg
    23281 gaggggtaca ttttgagaaa tctttctcag atattataaa aaataaagaa tataacatat
    23341 caattggaca tgtattgcca aatcaatgga ttgtaagtgg aatgtatgat gatgaatctg
    23401 gatttgagaa actagaaaag tgtataaata gtattgaagg aggaattgat tgtatagttt
    23461 ttcatgataa attaaaaggt acctataaag agcaatgtcg taaactaggt gaaaaactta
    23521 atgtacctgt atttaaacat aaaatattaa aaaatccaaa agacttacct atatggtagt
    23581 tttatacagt taatataaca ataagtcgga ttaattttta agcgaggtca aaaatgatat
    23641 tatcttattt tgcattatta attagagtag tgctattagc ttttgaaaga gtagttgtaa
    23701 gattattagg tgatgaagaa ggagatattt ataaaaatat agcttcatca tttctatttt
    23761 tctttatagg gggagtatgt ttacttccat ttagcataat ggatagagtt agtgattggt
    23821 cgtttttaat accatgttat ataagtagtt tagtctattc aattggttca gtagcgtatg
    23881 taacgtcttt agcaactggg gaagtttcac ttgttactcc aataaatagt ttaaattcat
    23941 tgtttttact gcttttatca gttatatttt taggtgaagg tttaagttta gcaaaagttg
    24001 caggaatcat aattatgatt ggtggagtgt ttatattaaa aaaagtaagc tcttttttaa
    24061 agaatgtctc tgtaattttt aacaatttac catgtagatt aatgttttta tacatatttt
    24121 tgcagtcagt aggaagagta ttggataagg ctttttttgt acaagtatct cctatattat
    24181 attcaactat actgtatttt ttcgtaggtt taaatttatt tatcttttta gcatttaaaa
    24241 agaaacaaaa aataatttgt gagattttta gtaataaaaa ggggctttca attttaagtg
    24301 gtgctattaa tggatattct tatttagcat tattaattgc tttaaataat ctagaactta
    24361 gtatagcaga gccattttct caagtatcta tgataataac tttaatactt gctcatttta
    24421 tattcaaaga aaatataaaa gaaaaaatac caggttcaat tttgatacta attggtgggt
    24481 ggctactgtt actttaattg atataaaaaa tgaattataa aggtgctatt agttagtatc
    24541 agaaaattta aaaaagtatt gacaattctt ttaggcaaat gttaatataa taatacaaaa
    24601 tagtataaat agaagacatg ttactggtaa tgcaggcagg atcgaatata ttgctttgat
    24661 ttttcaaggt gtttatatag gttctgcctt ttttgcatct tgaatggcca ttcataaaaa
    24721 attatggaga aggagattac aagatgaatt ataaaaaaac tgccattgac attattgaac
    24781 aagttggtgg ttcaaaaaat attttagctt tagaacactg ttcaactaga ctacgcttta
    24841 agttgattga tagaagtaaa gtcaatcagg atgctttaaa aaaagtacaa ggtgttatga
    24901 gtatagttaa tggggcacaa tttcaagttg tcattggaaa tagtgttgtt gatgtgtatg
    24961 atgaaatttt aaaagtttgc cctttagata caaatgatag tactgtaaaa actgaagaaa
    25021 aaataaactt aggaacaaga tttatagaat ttattattaa tatttttcaa ccactgattt
    25081 ttgctattgc aggagctggt attttaaaat cactgttgat gttattagca atgtttaata
    25141 ttttaaaatc agattctact atatatacat tattagttgc tatttcagat gcaacattct
    25201 atttcctacc tcttatggta gctgtaacta ctgccaatgt attgaaatgt aatcgtttag
    25261 tagctattgc cgctgtagga tacttattgc ttccagcaac aacaactgct ttaagtgaag
    25321 gtgtcgaact atttggattt acaattccaa acattgccta taatgcacag gttttcccgg
    25381 ctatcttatg tgtgtctttc ttggcaacaa tggagaaaat ttttaacaag tattcaccaa
    25441 aaccaattcg aactttcttt gttcctatga tgtctttggc aattactgtt ccaattactc
    25501 taactatttt aggtccttta ggatataacg ttggtacaat ttttacaacc attattttat
    25561 tcttatataa caaaatggga ttcttagttg taggattact tgctgctttt cttccattta
    25621 tggttgcaac aggaatgcac aaagcactaa taccttattc aattaataca attggaaagt
    25681 tgggttatga agctttatat atgacatctt cattagccca caatatttct gaaagtggtg
    25741 cttgttttgc agtagccttg cgaacaaaag atgaaacatt aaaacaaaca gcattatctg
    25801 caggaatttc tgccttgatg ggaattacag agccagcttt atatggtatt actttacaac
    25861 ataaaagagc aatgttaggt gttgtattct caagtgcaat ttctggtaca tttttaggat
    25921 tatttgcagt aaaaggattt gtaattgttg gaccagggct tgccagtatg agtatgttta
    25981 ttgacccaaa caatggaaac aatttgattt ttgcaagtat tggttttgtt attgcaatat
    26041 taggctcgtt tattattaca ctgattattt ggaaggaaga tacaaataca gtagagtcat
    26101 caattggaga tgttgttgaa gaagagctag aagaagaaaa agaagtaaat atcttagtaa
    26161 ctccagtaga aggaaaagta atagatttaa gtaaggttaa tgatgaacta tttgcaagta
    26221 agactttggg agatggagtt gcaattatac cgaccaatgg aaatttatac gcaccttgtg
    26281 attcagaagt tgtgatgtta tttgaaacaa aacatgcaat tggtttaaga acaaaaaatg
    26341 gggctgagat tttgattcat attggaataa atactgtaag tatgaatgga gatggattta
    26401 aaacatttgt taagacaggc gataatgtaa aagaaggaga tttattaatt cagtttgatt
    26461 tagacaaaat ctcacatgct aatttagatt caactgttat gattgtaaac aataatggag
    26521 cagattatgc ttataaagtg ttaaaccagt cttatggcaa tgtaaaaaaa ggaagtatct
    26581 tgtttgatgt aaagggagga atataaatat gagaaagttt ccagaaggat ttctttgggg
    26641 aggagctaca gctgctaatc aatttgaagg tggttggaat ttaggtggta aagggtggtc
    26701 tgtatcagat gtagcaaagg cacactttga tgcagatgtg aaagattata aatctaataa
    26761 tgaaatcaca acaaaagata tagaagaagg attagcacat cctgaagatg aagtaaatta
    26821 tccaaaacgt catggtagtg atttttatca tcactataaa gaagatattg ctctaatggc
    26881 agaaatggga tttaagacat atcgaatgag tattgcttgg tctcgtattt ttccaaatgg
    26941 agatgataaa gagccaaatg aagaaggttt acagttttat gatgatgtat ttgatgagtt
    27001 gataagttat ggaattgaac cacttgttac tatgtcacat tatgaaccac cattaaatat
    27061 cgttttaaac tatgatggat ggtattcacg acaagttatc aatatgtttg ttaggtatgt
    27121 agaaactatc tgtgaaagat ataaaaacaa agtgaaatat tggcttacat ttaatgaagt
    27181 tgattctatg attcgtcatc cgtacactac tggtggttta gttcgagacc gttttaagga
    27241 taaaaacttt gaggaagtta tttttcaagc tatgcatcat caatttgtag ctagtgccct
    27301 tgcaacaaaa atttgtcatg aaattattcc aaattcaaaa gtagggtgta tgttaacaaa
    27361 attaacgtat tatccataca catgtagacc agaagatgtt ttggcaactc aacgaaaaat
    27421 gagaagtatt tatgcatata gtgatacgca ggtttttggt gagtatccag tatatttact
    27481 gtcgtatttt aaaaataata acattcaaat agtaaaagaa gaacatgatg atgaaattat
    27541 gaaaaagtat ccagtagatt ttatttcatt ctcttactat atgtcttctt gtgaggcagc
    27601 tgatacaact ggattagata ttacacctgg gaatacgtta ttagctgtaa aaaatcctta
    27661 tttagaaatg agtgaatggg gttggcaaat tgattcaata ggtctacgta tatctttgat
    27721 tgaattatat gatagataca gaaagccttt atttatagtt gaaaatggat tgggagccaa
    27781 agatatttta actgaagata aaaaagttca tgaccaatat agaattaatt atttaaaaga
    27841 acactttaaa tgtatgttag atgcgattat tgaagatggt gttgaacttt ggggatatac
    27901 ttcatggggt tgtattgatt tggtcagtga atcaacgaaa caaatgtcaa aacgttatgg
    27961 atatatttat gtggatgctg atgattatgg aaaggggaca tataatcgat ataagaaaga
    28021 tagtttttat tggtataaaa aggtaattga aagtaatagt atagatttta aatgaccaat
    28081 agaaggtgag caaataaaat gaactacata ataaagaaag ttttgaactc gagcgttgtt
    28141 ttagttcatg acacaaagga taatgaattt attttgctag ggaaaggaat tggatatggt
    28201 aaaaaaacag gagaatatat ttgtggttcc aatgataatc aaatgtttgt ccctgttgaa
    28261 aatacaaaat caaaacaatt tttagaatta atggatgata ttcctgtcga cattttaaag
    28321 ataacacagg aaataatagt agaggcagaa aagttattga actcatcttt taataaaaac
    28381 ttatacttaa tattagcaga ccattttaac tttgcaattg aaaggatgcg taaaggaata
    28441 aaaattacaa atcgagtatt ttgggaaata aaaaattatt atccaaatga gtttaaggtt
    28501 ggaatggttg caatttatat ggtagaggag agattgggca ttcaattacc agaggaagaa
    28561 gcagctaaca ttgctttcca ttttgcaaat gctatggctt cagatggaaa ttcatatgat
    28621 acgattaagt atgccaaagt aattggagaa ataattaata tttttgtttt ttctttgaat
    28681 agaaccttag ataaaaagag tatgcattat atgagattta taacacatat aaaatttttt
    28741 gtggaaagat ttttttcaga tagtatgtta agcagtggtg atgatttatt atttaatcaa
    28801 atgaaaaggt caaatccaaa agaaatgtta attgcattaa aagttagaga ttttttagaa
    28861 aagaaatatg gaaaaaagtt aacaaatgag gaaatagctt tcttggttgt ccatatcgca
    28921 cgagttaagc aatagagtga attactaata ttatcttata cgtctacatg gtagaaaatt
    28981 gaaagaaggt aaagtatata aagaaactga gacatattat tttgttgttc attatgttta
    29041 ttttatgagt gtatcctgtt aaaaatttaa gttaatattt ataattaaaa tatacaaata
    29101 gttaaaa
//
LOCUS       DAMJWU010000041        27317 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMJWU010000041 DAMJWU010000000
VERSION     DAMJWU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.041"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            264..797
                     /gene="pyrR"
                     /locus_tag="PC460_001979"
     CDS             264..797
                     /gene="pyrR"
                     /locus_tag="PC460_001979"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2600865.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            1081..2292
                     /locus_tag="PC460_001980"
     CDS             1081..2292
                     /locus_tag="PC460_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2600866.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2591..4243
                     /locus_tag="PC460_001981"
     CDS             2591..4243
                     /locus_tag="PC460_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2600867.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(4458..6233)
                     /locus_tag="PC460_001982"
     CDS             complement(4458..6233)
                     /locus_tag="PC460_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2600868.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6529..7563
                     /gene="ltaE"
                     /locus_tag="PC460_001983"
     CDS             6529..7563
                     /gene="ltaE"
                     /locus_tag="PC460_001983"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2600869.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            7850..8680
                     /locus_tag="PC460_001984"
     CDS             7850..8680
                     /locus_tag="PC460_001984"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2600870.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            8684..9565
                     /locus_tag="PC460_001985"
     CDS             8684..9565
                     /locus_tag="PC460_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2600871.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            9595..10212
                     /gene="gmk"
                     /locus_tag="PC460_001986"
     CDS             9595..10212
                     /gene="gmk"
                     /locus_tag="PC460_001986"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2600872.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            10215..10481
                     /locus_tag="PC460_001987"
     CDS             10215..10481
                     /locus_tag="PC460_001987"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2600873.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            10486..11685
                     /gene="coaBC"
                     /locus_tag="PC460_001988"
     CDS             10486..11685
                     /gene="coaBC"
                     /locus_tag="PC460_001988"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2600874.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            11924..14449
                     /gene="priA"
                     /locus_tag="PC460_001989"
     CDS             11924..14449
                     /gene="priA"
                     /locus_tag="PC460_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2600875.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            14467..14907
                     /gene="def"
                     /locus_tag="PC460_001990"
     CDS             14467..14907
                     /gene="def"
                     /locus_tag="PC460_001990"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2600876.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            14922..15851
                     /locus_tag="PC460_001991"
     CDS             14922..15851
                     /locus_tag="PC460_001991"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2600877.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            15861..16613
                     /locus_tag="PC460_001992"
     CDS             15861..16613
                     /locus_tag="PC460_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2600878.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTVVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            16682..17383
                     /locus_tag="PC460_001993"
     CDS             16682..17383
                     /locus_tag="PC460_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2600879.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            17445..18770
                     /gene="rsmB"
                     /locus_tag="PC460_001994"
     CDS             17445..18770
                     /gene="rsmB"
                     /locus_tag="PC460_001994"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2600880.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            18778..19809
                     /gene="rlmN"
                     /locus_tag="PC460_001995"
     CDS             18778..19809
                     /gene="rlmN"
                     /locus_tag="PC460_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2600881.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            19829..20581
                     /locus_tag="PC460_001996"
     CDS             19829..20581
                     /locus_tag="PC460_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2600882.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            20598..22613
                     /gene="pknB"
                     /locus_tag="PC460_001997"
     CDS             20598..22613
                     /gene="pknB"
                     /locus_tag="PC460_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2600883.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            22721..23458
                     /locus_tag="PC460_001998"
     CDS             22721..23458
                     /locus_tag="PC460_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2600884.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            23556..24440
                     /gene="rsgA"
                     /locus_tag="PC460_001999"
     CDS             23556..24440
                     /gene="rsgA"
                     /locus_tag="PC460_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2600885.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            24445..25116
                     /locus_tag="PC460_002000"
     CDS             24445..25116
                     /locus_tag="PC460_002000"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2600886.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLSDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            25125..25766
                     /locus_tag="PC460_002001"
     CDS             25125..25766
                     /locus_tag="PC460_002001"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2600887.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            25897..26526
                     /locus_tag="PC460_002002"
     CDS             25897..26526
                     /locus_tag="PC460_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2600888.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            26581..27237
                     /locus_tag="PC460_002003"
     CDS             26581..27237
                     /locus_tag="PC460_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2600889.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT        10883 a         2764 c         5093 g         8577 t
ORIGIN      
        1 ttaaaaaagc ttgaaaaatt tgaaatataa atatataatt tgatttgata gattaaaaaa
       61 ttatctttaa acgatacaga gatatcgtaa gacaattgct aaattgcact gtatattttt
      121 tatataagag cttcttgtct tgtgatgaga agtttttttg ttgtttaaat tgaaatttta
      181 aataaaaata aatttataaa aaattaaaag aaaggagtag atttgtcaaa tagatatatt
      241 atatctttga caataattat gatatggtag aaaaagctca attaatggat gaaaaggcaa
      301 tagcaagagc aattactaga ataagtcatg aaataattga aagaaataaa ggtgtagaaa
      361 atctagtttt agttggaata aagactagag gtgtgccaat tgcaaataga atttctaaga
      421 aaatagaaca aatagaagga acaaaagtag atacaggaga tattgatata actttgtata
      481 gagatgattt ggaaaaaatt catgttgaac ctgtagttaa aggaacttat ttagatttta
      541 atgtaaatga taagacagta gttttagttg atgatgtttt atacactgga agaactgtga
      601 gagcttcact agatgctatt atagatatag gaagacctaa atcaatacaa ttagcagtgc
      661 tagtggacag agggcataga gagttgccta taagagcaga ttatgtagga aaaaatgtac
      721 ctacttctag acatgaaatt atatctgtca gtttattaga aattgatgga gaagattcag
      781 ttacaatcaa agaataatta tttttaagta ttatataaaa gcttagcatt gaatagtcag
      841 ggaggaggat aaagcttaca tagattgcac aaaataaata ttataatttt atggatgtga
      901 gtcttaatgt agtttttatt ctcctttatt tgagtttttg atgtcgtaaa atgtaaaata
      961 atgataaatt atataataag tatatagagg ttaaaaaata gaatctaggg aatgataatt
     1021 aattaaaaga tattaattaa acagaggatt tatttatata taataatttt ggaggttttt
     1081 atgaaaaaaa cgataatgtc tttacaacat ctattagcca tgtttggagc gacagtatta
     1141 gttcccattt tgacaggttt taatccgtca gttgcaattt tctgtgctgg tgttggtaca
     1201 ttgatatttc atttttgtac agaaggaaaa gtacctgcct ttttaggttc aagttttgcg
     1261 tttataccag ttatactagc agctaaagag gcatatggtg gagatttagc atatgctcaa
     1321 ggtggtataa tagtagcagg attaatttat attataatgt cgattatagt aaaagtagtt
     1381 ggtgtaaata aaattaaatt atattttcct gcacaagtta caggagctat gatagtagtt
     1441 ataggattaa atttattgcc aacagctttt agtatggctt cagccaattt tgtgatagca
     1501 tttataactt tagcaatagc aattttaacc aataagtttg gtagaggatt tataaaacaa
     1561 ttaggaatat taatagcagt tttttctggg tatataatct gtttgatatt aagattagta
     1621 gatataacta ctattacaga agcaagttta tttgcaatac ctaactttac agttcctaag
     1681 ttcagtttag gagcaatagt gattatatct ccagttgtat tagctgtatt tatggaacat
     1741 ataggagata tgactactaa tggtgctgta gtaggtaaaa actttataga aaatcccgga
     1801 ttaaatagaa cattacttgg ggatggattt gcgactatag ttgcagggtg tttaggagga
     1861 ccagccaata caacttatgg agaaaataca gcagtacttg ctataactaa aaactatgac
     1921 ccttcaatac ttagacgtac agcaatattt gctatattgc ttgcatgtgt aggcaagttt
     1981 ggtggatttc ttcaaagtat accaggttca gttatgggtg gaattagtat aatgctattt
     2041 tcgatgataa cttatgtagg attaaaaact ataagggaca gttcttgtgt agaaagtaaa
     2101 ataaatatat taatcatagc agttatatta ttaataggtc ttggaacaac atatttatct
     2161 aataaaggta tttctgttgg aatccctata acaagtacag taaagataac tggtcttagt
     2221 ttagctgcaa ttgttggaat tgtattaaac agaattttaa ataatcagga ctttaaagtt
     2281 gaagaagaat aaatttaaag aataagttgt atattgaata actaaaatta gttaattttc
     2341 tatagacatt tgtaattaat gttgatataa tgattaaaaa taaatgaaag aaagggcaag
     2401 gtaactagga caatgagaat ataatctatt ttttataatt taaagtattg taatttttga
     2461 aaagcctatt ttggtggctt tatttgcaat gcttaaatta aggagagtag attatagtct
     2521 atacgtttat ttgttatgcc tatttataga atctctcctt atatttaagt gtgttaagga
     2581 gggatttttt atgttgttag taaaagtaga gaatttaaag aaatattatg cagataaatt
     2641 aattttggat atagataaac ttgaaatatt agaaaatgac aaaataggtc tagttggttc
     2701 aaatggtcaa ggaaaaacaa cattattaaa agcaatattg ggagaaatag aaattgatga
     2761 aggatatact taccttacag aaagctattc ttatataagt cagagtgaaa ataatattga
     2821 aacatgtagt catagtaagg agaagagtct tttaaatgct ccagataaat ttgaagaaca
     2881 cttatcagga ggggaaaagg ttaagttaaa aatagcagat gcgctgagta ataagaaaaa
     2941 tatcataata gctgatgagc caacttcaaa cttagataaa aaaagcattg gggttttgga
     3001 agatatgttt aaaaggcatg agggagcatt attattaata tctcatgata gacgttttct
     3061 ggatgagtta tgtacaacta tattagaact agaagatgga aaactaaaag cttacaaagg
     3121 caattacact gattatttaa tgcaaaaaga tgaagaagta aaaagagctg attttgagta
     3181 tcaagaatat gttaaagaga aaaaaaggct tgaaaaagct cttttatata aaaaagcttt
     3241 aagtgatggc ataagaaaaa caccaaaaag aatgggtaat tcagaagcta ggttgcacaa
     3301 aatgggtggt cagactaata agaaaaagtt ggactcaaat gtgaaggcta taaaaagcag
     3361 aattgataaa cttgaggtaa agaataaacc taaagtttct aaagaaatga acattaagat
     3421 gcaagatggt atggagataa ttagcaaaaa tctagtagag gtaaaagata tgactctaaa
     3481 gttagaaaat aagctcctgt tagataatgt ttcctttaag ataaaaagag gtaaaaagat
     3541 agcattgtta ggtgataatg ggtgtggaaa aagtactctg ataaaagaaa tattggctga
     3601 taaaaatgac aatataaaaa taaataacaa ggtaaaagta ggttattttg accaaaatca
     3661 aagtttatta gatgaggaaa agagtgttct atacaatact aaagttaata gttcatttga
     3721 tgaatctttt ataaggataa acttaagcct atttgggttt aaaggtgatg atgtctacaa
     3781 aaaagtaaaa gtacttagtg gtggagaaaa ggtaaaaata gcactatgta aaataatatt
     3841 ggaagataat aattttttag tatttgatga accaacaaat tacttagata taaaatctat
     3901 ggaagcactg gaaaaggcac tgataaatac tgataaaact atgcttatag tgtctcatga
     3961 tagagtattt gtatcacata tttgtaacta tattatagag ataaaagatg ctaaaataag
     4021 ggagtttgat tgtaattatg acgaatatac aattagcaga aataaaaaaa ctcctagtag
     4081 agaaaaccaa atcaaaaaag aaaatcttct agtattagaa aatagactta caactgttat
     4141 atctatgtta tctatagaaa aagataatct aaaaaaagaa ttgtatgaat cagaatataa
     4201 tgaattacta aaacaaatca ataaattaaa aaatagcttt tagtgttaac tttttaatta
     4261 catctatata agaagactga aaatatttgt tgggaatata agtatagtta ttaaaacaga
     4321 aaagtatttt gttagaaaat taaaagtaag ctaattagaa agtggcctta ttattgtact
     4381 ttataagaag gccactattt ttatttagaa tgataatgtg tagttgtcta acatttattt
     4441 tgtatttagt gcagaattta ttcagattta accttaagct tggctactgt ttcttcttca
     4501 ggagtaacat aaatagtact gttagtttca taaataacca tacctggttt agaaccacta
     4561 ggttttttca cattttttct cttagtatag tctacaggca cctgagaaga aagttttgac
     4621 ttactaaaga atgctgctag catagctcct tcaaatacag tattatcagg gacttcctta
     4681 ccagcacact ttattattac atgagaacca ggaatatttt ttgtatgcat ccataagtca
     4741 tcattatcag caagtcttag tgtaagatag tcattttgct tattgttttt accaaccaat
     4801 attttaaatc catcagaaga cacaaattca tgaggttttg tagatgggat agtatccttt
     4861 ttactgtttt tcttttgagt cttgatgtat ccaactttag caagttcttc ttttatatct
     4921 tgtaattcag ctaaattttc acagttttca atacttaaaa ttatgttttc taagtaatct
     4981 atttcttcct tatttaagga aatttgatga gatatctcga ctttagcatg tttcattttg
     5041 ttatactttt taaagtattt ttgagcattt tcagatggtg tcaagttttt atttaattct
     5101 attgttacat cattacaatt ttcatcatag aagttagcaa cttcaatact ttccatacct
     5161 ttttctacca tataaatata tgaagtaatc aattctcctt ttatcttata aatgtctgca
     5221 ttttcggatt ctgataattc ttcttcttgt tttttaagtt tattataaag tctatcaagc
     5281 ttaacagata tactcttctt taaatcagat gaacgttgat taattctatc tttaatatcc
     5341 ttagttctat agaaatcttc cagaatacga gacatactat ccttatttat atatgataaa
     5401 tcgctaaata atgtaaggtt tatacaactg aaatcaacta ccttatccac ctttttatct
     5461 ataataatgc aaggagagta tttgttagaa tttatatcat tgaataaatt acaaaatact
     5521 ttgtgtaaag catcaaattg ttcatcagat atatccttta tgatagcatt ttgatttaca
     5581 cctgctctgt agcaaatttc tttagctata atagggctta tacctagaaa tttagaatat
     5641 atagacttaa agataggtcc ttccaattcc tctaggtttt taataaacaa atccttagat
     5701 atatcatcaa gagggttgag tttgttttgt tctggtggca atgaataatc atgaccaggt
     5761 aaaacttgac gaactctact tatgctaaaa gggactcttt ttatagaatc tataatttta
     5821 ttttcttcac catgagttat tattatgttg ctatgtcttc ccataatttc aatcataata
     5881 tttttaactg tcttttcttt taattcatct aaagattcaa cacttatttt tattattctt
     5941 tcaaaaccta tttgagaaat ttctactata tttccacctt gtatgtattt tctaagtaac
     6001 atacaaaaca taggcgcttt tagagggttt tctttttgat aattacttgt aagataaact
     6061 ctaggatttg aggcacttgc acttaaaacc aatctaaaat tttctttatt atttcttata
     6121 ttaaaaatta cctcatcatc ttcaggttga tgtatcttat ctatttttcc acctgtaagt
     6181 tttgatgata attcatcgac tattgaatgt ataactaatc catctaatgc catttatagc
     6241 acccctttct ttgaccaaaa tgagtcagta aattaattat acacataata tacgctttgt
     6301 gactaggggg aatttaaaca atagagatta atatttaaat tgtataatta tttatattta
     6361 ttagagagga aactttattt ttaaaattaa taatttaaag tagattaaat ataaagtaaa
     6421 tatagtataa ttattgtgta gagtataagc tatgaaagta tatgaacttt acagaaaaaa
     6481 tcaattttaa ggttctatag tatataaata tgaaagaagg ttgaaaaaat gagatttatc
     6541 gatttaagaa gtgatactgt tactatgcct actgacgaaa tgagagaagc tatggcaaag
     6601 gctcctgttg gagattcaat ctttagagat gaccctactg taaataaact tgaagaatta
     6661 gcagcagcta aagttggaaa agaagatgct attttccttc caagtggaac ttttgggaat
     6721 caattagctc tatttactca ttgcctaaga gggcaagaaa taattattgg taaaggttat
     6781 cacattgtga ctcatgaggt tggggcacct gctgtaatag ctggagttca gcttagaact
     6841 atagatgaag acgaaagtgg agctctaaat cctgaacttg ttgaaaaggc aattagaaga
     6901 gatgacatac atgaaccaca gacagggctt atatgtgtag aaaatgcata ttcaggtgga
     6961 acagttgtga gtcttgataa tttaagagaa attaaaaaaa tagctgagaa acataactta
     7021 ccagtacatt tagatggagc aagattattt aacgcagcat taactttggg tgtagaagct
     7081 aaagaaatag caaagtgttg tgatagtgtt atgttctgtg tgtcaaaagg tttagctgca
     7141 ccaatgggtt ctatacttgc tggaaataaa gaatttatag caaaagctag aagaaagcaa
     7201 aaagttatgg gtgggggaat gagacaagta ggtatagttg cagcagctgg tataattgcc
     7261 attgaaaaaa tgactttaag attgaatgaa gaccatgaaa atgcaaaata tttagcttgt
     7321 gaattaaata aaattgatgg cattgaagta aacaatgtta atccagatat aagtatggta
     7381 ttttttaaga tgagtgaaga tattataaaa gaggatgttt taataaaaga attttttgaa
     7441 agaaatataa aaattaataa aatggaaaat ggtgaatata gatttgtaac tcatgtagat
     7501 attactaagg aagatttaga ctatgtttta aaaacactta aagaattaat aggtgtatgt
     7561 taatagatat ctactagaag gattatagct agttaccaga tgtttaatta aaaaattcat
     7621 agacagttta aataaaacat acataaaatt catatagaaa tattaatata taaacataga
     7681 aagaactagc ttaaaaacat aatataatta ccctaattgt aatggcaact ctccctcaca
     7741 tcctcaaatt atagttgtca ttacaaaact tttcccataa attataagtt tatgggaatt
     7801 tttttatgtt ataatagttt aagcgttaat ttacgaagga gcgatactta tgaaattttg
     7861 gaaactacat ggaataggaa atgattttat agcaatagat ggaagattcg accagataaa
     7921 tccatctgat tatactgctt tagcaaaaaa agtatgccat agaagatttt ctgtaggagc
     7981 ggatggattg ttagttgtga aagattcaaa agttgcagat gtagaaatgg tatattataa
     8041 ttctgatggt tcaagagcag ctatgtgtgg aaatggcctt agatgttttt gtaaatttgt
     8101 ttatgataat tgtatagtta aaaaagaaag ttttacagta gatacattag atggtataaa
     8161 agatataaaa gtaaatctta ctaatagaga aataaactct ataagagtca atatgggaaa
     8221 aggaagtttt atagctaagg acgttcctgt acttacaaat aaagaacgtt ttgtgcaaga
     8281 aagtataaag gttttagata gggagcttaa agttacttct atgttgatgg gggttcctca
     8341 tacagtagtt atagtagatg aattagatgt agatgatgtt tgtaaatatg gaaaagagat
     8401 tgaaaataat aaaatatttc ctgaaaaaac gaatgtaaat tttgtaaagg tagaagataa
     8461 gtataatata catgtttata cttgggaaag aggttgtgga tatactttag gttgtggtac
     8521 tggtatgact gcatcagcaa tagtgtgcaa tttacttaat atggtagaaa gtagtgtaaa
     8581 tgtaacgtct caaggtggaa cagttaaaat agatgtagga gatttctctt atatgacagg
     8641 tccagcagta aaaatatgtg aaggtatttt ggaggtttaa ttaatggcta taagtatgac
     8701 tggttttgga agaggagaat acaaggatga taattattac tttttagtag agtgtaaaac
     8761 gataaatcat aagtattcag atataaacat acgtcttcca agaaaaattt catttttaga
     8821 ggataaagtt agaaatttag taaaaaatta tgtaaaaaga ggaagagtag atttatacat
     8881 aaagtttgac ttattaggca aagaagatgt aaacttaaat tttgatgagg gattagcatc
     8941 tcagtatata gacatactaa aagaaataaa aaataaattt gatattatag atgatattag
     9001 cgttatgaat gttgcaaagt ttcctgatat tgtgaaaata gaagaaaagg aagaagatga
     9061 agatttattg tggtcaatgt tgaatcaagc agttgaagat gcattaatca aattaagaga
     9121 aatgagaagt gaagaaggta aaaaattagc tgaagatatt gccatgagat gtgatttatt
     9181 aaaaaatcat atagaagaga ttgaaaaata ttcaagtagt gttgtagaag attatagaga
     9241 aaaattgaat ttgagaataa gtgaattact ggatgaccca agtataatag atgagaatag
     9301 attagcacaa gaagttgcaa tatatgcaga taaaagcagt ataacagaag aaatagttag
     9361 atttaaaagt catatagggc aacttaaaaa tactatattt aaagacgact ctataggtag
     9421 aaaaatagat ttcttaatac aagagatgaa tagagaaact aacactatag gttcgaagtc
     9481 atctgatata aatataacta atttagtagt agaagtaaaa agcgaattgg aaaaaataag
     9541 agaacaaata caaaatatag agtaagaata caatattaga aggggaatgc ttaaatgttt
     9601 gtgaaaaaag gattattgct agttgtatca ggaccatcag gtgcaggaaa aggtactatt
     9661 tgcaaagaac tacttaaaga aaatgataca ataaaacttt ctgtatcagc tactacaaga
     9721 aaacccagaa ctggagaagt tgatggagta aattatttct tcataagtaa ggaaaagttt
     9781 gaagagatga tagaaaaagg tgagtttttg gagtatgctc aaatttatga taatttttat
     9841 ggaactccaa aagcggcaat aatggaatgt ttagaaaaag gacaagatgt attactggag
     9901 attgaaatgc aaggagcaaa acaaataaaa gaagtttgcc cagagggagt atttatattt
     9961 gtactaccac catcacttga agaattaaaa aatagaattg ttggaagagg tacagaaaca
    10021 gaagctgaaa tagagaagag atttagctgt gcatatgaag aaattaaaat gattaaggat
    10081 tatgattatt ttatatttaa tgaagatgta aaaacatctg ctaaagaaat agaaggtata
    10141 atatcatctg aaaagaataa agttagcaga tataaaaata ttataataga gaaatttaag
    10201 gaggaattat aattatgtta aaaccatcta tcaatgaggt actagaaaaa atagacaaca
    10261 gatactattt agtggggact gtttcaaaga gagcaagaaa attaattgat ggagaagaac
    10321 catatgtttc aaataaaact aaggaaaagc ctgtatgtgt agcaactaaa gaagttgcaa
    10381 gtggaaaaat aacatataga ttattgacag aagaggaaat agagatagaa gaagctagac
    10441 atcatgctga gcaacaccaa caaatttcag aagaggagta aggtcatgct aaaagataaa
    10501 actgttgtaa ttggcataag tggcggtata gctgcatata aggcttgtga tgttgtaagt
    10561 aagttaaaaa aactaaatgc aaatatccat gttataatga caaaatcagc aacagaattt
    10621 gttaaaccac ttacatttca atcgctgagt caaaattatg tagtagaaga tatgtttgaa
    10681 gaaccaaaaa cttgggaagt tgaacatata tcattagcaa aaaaagcaga tttattctta
    10741 atagtgccag caagtgcaaa tgttataggc aagattgcaa atggtatagc tgacgatatg
    10801 ttgacaacta ctgttatggc aacaaaagca caggttctta tagcaccagc tatgaatact
    10861 aatatgtatg aaaatccaat tgttcaaaaa aatatacaaa ctttaaagag ctttggatat
    10921 aaatttattg aaccggaaag tggaaggctt gcatgtggag atattggaac aggtaaacta
    10981 gctaatccag atataatagt agaggaagtt ttgactaatc ttactaaaga gcaagattta
    11041 aaaggtcaaa gtatgatagt tacagctggt ccaactgtag agagtataga cccagtaaga
    11101 tatttaacta ataggtcttc tggtaaaatg ggatattcta tagcaagaga agctatagct
    11161 agaggtgctg atgtaacatt aatatcaggt cctacaaatc tttctattcc acaaaatctt
    11221 aaaaaatttg ttcaaataga gtcagcacaa gatttgtatg aagcagtagt atcaaattta
    11281 agtgaaaata aaatagtaat tcaaagtgca gcagttgcag attataaacc aaaaacttat
    11341 tctgacaaaa aaattaaaaa gaagaatgat gatttatcaa tagaactaag tagaaattat
    11401 gatattgctt atgaaatagg aaaaataaaa gaagataaga tactaattgg atttgcagct
    11461 gaaactaatg accttataga acatgccaaa ggaaaaataa aaaagaaaaa tttagatttt
    11521 attgtggcaa atgatttaac taaagaaggt gctggtttta gagctgatac taatattgta
    11581 aaaataatag atgtagaggg aaatattact gagtatccta agatgaaaaa agaagaagta
    11641 gccaatgtta ttttagataa agttaaagcg ttgcttgaga aataagacct tttttaaata
    11701 tgatatttgt gtattttatc acagctaaag tggctgaaag ctgcaattta attttagcga
    11761 gtaaaaactc gcaaatgtca gttaaattgt agttttcagc atttattttg tcataaagta
    11821 taaaatataa ttaatagtta attattaaaa taaagttaat attaataact tgaaattgct
    11881 tataaattct gagattttaa tatcttgtaa agtatattaa aatatgaaca atatattaaa
    11941 tttaagtaga ggttttataa tgaaaaaata tgcaaaggta atagttagaa gtaatactat
    12001 atatacagat aatttattta catatcaaat tcctgtattt ttatctgatg taataaaaat
    12061 tgggcacaga atattagtac catttggaaa aggtaataaa ccaacagaag cctttgtatt
    12121 tcaatttaca gatagcttag atgaaaaaat aaaaataaaa gaaattattg atatattaga
    12181 tgaaaatccg atatttagaa aagaagattt agagttggtt tattggatga aaaatagata
    12241 tttgtgtact tatatagaat gtataaattt gatatatcca aagggataca aattaaataa
    12301 ttataaagta gtcctattag gtgaaagttt aagtggatta aatgatattg aattgaagga
    12361 aaaaatatca actttaagtg ataaaaatag agaaatagta gagaaagtga ttgatagtaa
    12421 aggtaaaatt aaggttgata agttaaaata tataccaaac ttaaatagtt ctttgtacac
    12481 aatgaataaa aatggaatta taaaattatg ttgggaatat aaaaatcaca aaaatgaaaa
    12541 gaaagtatgt tatatatctt tgagtttaga aagtgataag attgacgatt atatagagca
    12601 aaataaaata aatgtgggaa gtaaacaaaa agagatatta agttttctta aaaataatga
    12661 aaatgtagag ataaatgatt tattggattt attaaatgct tcaaaacaaa gtataaattc
    12721 tctaagtaag aaaaagctaa taaccctaga atttaaggat tattatagag agccaaaaag
    12781 tatttacaaa tccgtactaa aaagtataaa attaaacaat gagcaacaag aagcagttga
    12841 tgagataaag tcgaatatgt ttgtagatga taaaaaacca tacttaattc atggagtaac
    12901 tggaagtgga aaaactgaag tttacatgga aataatagaa tttgctctaa atcaaggctt
    12961 agacagtata ttcttagtac ctgagatagc attaactcct caaacaatag ataggctaaa
    13021 gagtaggttt ggagatttag ttggagtgtt ccacagtaaa ctatctgaag gagaaaaaca
    13081 tgacgtttac aaagctgtca aagcaggtaa agtaagagtg ttaattggag ctaggtcagc
    13141 tttatttgca ccttttaatt ccttaggctt aataataatt gatgaatgtc atgagtcatc
    13201 atataagtct gagaagaatc caaaatttaa tgctatagaa gttgctagat ttatggcatt
    13261 aaaaaataat ataactctta ttttaggttc agcaacgccc tcaattgaag agtattatag
    13321 ggcaaaatct ggagaataca aacttataaa tataaaaagt agagcaaatg ataagcctct
    13381 tccaaacatt gaagttgtag acatgaaaga tgagttagat aaaggaaata gaagtatatt
    13441 tagtttgaaa ttacaaaaag aaatacgtta tgcgattgaa gaaaataatc aagtaatact
    13501 atttttaaac agaagaggtt atgcaaattt tgtatcttgt agaaagtgtg gatatgtgtt
    13561 tcaatgtgaa aattgtgata tatctttaac ttatcataag aaaagcaata ctggaagatg
    13621 ccactattgt ggatatgaga aagaaattcc aaaagagtgt cctgaatgta aaagtactta
    13681 tgttaagcca tttggagttg gaactcaaaa aattgaagag gaattaaaat atatttttcc
    13741 agatataaaa actttgagaa tggataaaga tacaacttca aaaaaaggtg ctttagatga
    13801 gatactaaat aaatttaaag ataaagaagc agatgtactt attggaacac agatgttaag
    13861 taaagggctg gattttgaga atgtaacttt agtaggtatt ttgtctgcag acatgatatt
    13921 aaattttcca gactttaaaa gttttgaaac tacatttcaa ttgataactc aagtctctgg
    13981 aagagcaggg agagctgata aagagggaaa agtagtactt cagacttatg acactgaaca
    14041 ctatgctatt aagcatgcta ttgaatatga ttatgagggt ttttatgaag atgagataaa
    14101 aataagaaaa gcatttggat attctccttt taataatatg ttaagtgtag ttgttagtgg
    14161 tgaagatgaa agattagtga taaaaaatat aaaaaatatg catgcatcat tgatttattt
    14221 attagaaaaa aggggtataa atgatttagg atttatatta ggtccaaatc catgttcaat
    14281 atcaaaaata aatcaaaatt atagatggca aatactcttt aaagatgaaa atattgaaat
    14341 taatctctta aagggtataa taaaatatat atgtataaca aaacgagatt tgatatttga
    14401 taagaatatc aatgtatcta tagatattaa tccaaacagt gtattataaa tttaggagga
    14461 attataatgg ctttaagaca aatagttcaa ataggagaac cagtattaag aaaaaaatca
    14521 aaaaaggttg aaaaaattga tgaaaagata atacaattat tagatgatat ggcagaaact
    14581 atgtatgatg cagatggtgt tggactggct gcacctcaag ttggaatatt aaaaagagta
    14641 gtagtaatag atattggaga agagcttata gaacttataa atcctgaaat aatagaaaca
    14701 tctggggagc aaatagatga ggaaggatgt ttaagtgttg ttggagaagc gggaaatgtt
    14761 agaagaccaa attatgtaaa ggtcagagct ttaaatagaa atggtgaaac aatagagtta
    14821 gagggagaag aacttttagc tagagcattt tgtcatgaga tagaccactt agatggaatt
    14881 ttatttgttg acaaaataga aaagtagtag gagttgattt gatgaaaata gtatttatgg
    14941 gaactcctga tatagcagtt ccatgtttac aaaaaataat agatgagaag tatgaaatat
    15001 tgggtgttgt aactcaacca gataaaccaa aaggaagagg taaaaaacta ggtatgagtc
    15061 cagtaaaaga actggcaatt gaaaataata tacctgtata tcaaccagta aaggctagag
    15121 ataaagaatt tatagataaa attaaatctt taaatccaga tgtaatagta gttgtagctt
    15181 ttggacagat acttccaaaa gaaatattag agattcctaa acttggatgt ataaatgttc
    15241 atgtttcttt acttccaaaa tatagaggtg cggcacctat aaattgggta ataataaatg
    15301 gtgaagaaaa gactggtgtt acaactatgt atatggatga aggattagat actggagata
    15361 tgatactaaa gacagaagta aatcttgatg aaaatataac agcaggagaa cttcatgata
    15421 aaatgatgaa tataggagca gaaactttaa aagaaacatt aagattaatt gaagaaggaa
    15481 atgctccaag agaagtacaa aatcatgaag aattttccta tgcgccaata atgaataaat
    15541 cattaggtaa catagatttt tcaaaaagtg ccagagaaat acacaattta gttagaggag
    15601 taaatccatg gccaagtgct tatactacct ataatgatgt cataatgaaa gtttggaaaa
    15661 ctaaagtgtt agatgaaaaa agcacaaaag atgtaggtac aataattgat gtaagcaaag
    15721 atgggataaa agtaagtact atagataatg tacttttaat tgaagaaatt cagatgccga
    15781 ataaaaagag aatgttggtt ggtgaatata taaaagggaa tactatagaa actggtttag
    15841 tattgggtta ggtgaaaagt atgacagata taaagaagta tttaacaagt gttgggattt
    15901 tgattttaat attaggaatt ttgataggag ttgttaattt ttttataata agtttaactc
    15961 aaatattctc cttgatgata aatattataa taatatcact aatacttata atactatgtt
    16021 caacagtagt tacttataga gtaattaaag gaaagtatgt taactcaaat ataatgaaaa
    16081 taaattttaa tattgtcagt ggattatttc ctattatatc tttcattgca tcatcatttg
    16141 gtatgtcaaa aagtgatatt agaagaatat atataaagtt aaataatgaa tatatataca
    16201 gtaataaata taattttaat ccagaggata taataatttt aatacctcat tgtattcaag
    16261 agaatagttg taaattaaaa gttactaatg atatagataa ttgtaaagaa tgtggaagat
    16321 gcaacatagg agaattaatt aagctaaagg aaaaaactaa tgtaaaaata tttgtagcta
    16381 caggaggaac tctagctaga aaaattataa tggacactaa acctaaagct gttgtagctg
    16441 ttgcatgtga aagagatttg acatctggaa tacaagatat taagaaaatt cctgttttag
    16501 gtgtatttaa caaaagacct aatggacctt gtgtaaatac taatgtagac atgatggata
    16561 ttgaaaaagc tataggcttt ttaacaggaa aaaatatatt agtttgtaat taaactgttt
    16621 ttaagttaag tgaatattaa gaggatgtat aataaaaata tattttagga gagtgttatt
    16681 tatgtaccct gtatatggcg gtttttgggg atttgaccca acaatggttg tattaattcc
    16741 agctatatta cttactatat atgcacaatt taaagtgagt tcaacaacaa ataagtattt
    16801 aagagtaaat acacgtcgag gatatactgg agagcaaaca gcaagaagag tacttgattc
    16861 aaatggtttg tatgatgtaa agatagaaat ggttagaggg catctaagtg accattatga
    16921 cccaagaaga aaggctgtaa ggttgtcaga agatgtatat tatggaacat ctataacttc
    16981 tgttgcagta gctgcacatg aatgtggtca tgccatacaa catgctaaag gttatgcacc
    17041 tcttcaaata agaagtagtt tagtaccagt agtgaatttt gcttcaagta tatcttggtt
    17101 tttaatattt ttaggattta ttatggctgg tccattttta aaaattggta tactattgtt
    17161 ttcggcttca gtgttattcc aaataataac attgcctgta gaatttaatg cttcaagtag
    17221 agcaatcgtt caacttggga atttaggtat tatagatgaa agtgagtcaa gacaaagtag
    17281 aagagtactt tcagcagcag ctttaacata tgttgcagca gcattggttt caatacttca
    17341 attacttaga ttgttactaa tagctcaaag aagaaatgac taaaagtaaa gtgggttgtt
    17401 tagaaacaac ccttttttat gtttaataaa agggagaaat ttatatggat gcaagagaag
    17461 ttggatttaa agtattatgt gatatagaaa aaaatgacaa ttattcaaat attgcaataa
    17521 ataagcattt taaaaattta gaaataagtg atatggatag aggacttgca acagagttaa
    17581 tttatggagt aatagaaaat aaatattatt tagattatat aatcaataaa ctttcaaaaa
    17641 taaaggtaaa aaaaatgtca acttatgtta aaatcttttt aagaatggga acatatcaga
    17701 ttttattttt agatagtata tcagattatg cagcagttaa tgaaactgta aaattatcta
    17761 agaaatatga taaaaaatct tctggtttca taaatgctat tcttagaaat gaaataagag
    17821 ctaaagagac tataatggac ataactgagg aagatagtgt aaaatactta tctataaagt
    17881 attcctataa ctcttggatt ataaaaaact ggatagataa atttggacaa gaatttgctg
    17941 aagatttgtt ggaagcaaat aatgaaaagc ctagtatcta tataagaacg aatacactta
    18001 aaataagtag agaagaactt attgaaaaat taagtgaaat gggtattgta tgtttaaaag
    18061 ttcctatggt agaagaagct attaaagtcg aaaaaatgaa aaatatagaa aaaaatgaat
    18121 tatttaaggc tggtttattt acaattcaag atataagttc tatgatagta gggaaagtaa
    18181 ttaatccaaa agaaggctca tttgttttgg atgtatgtag tgctcctgga gggaaatcta
    18241 ctcacttagc tactttaatg aacaatactg gtcaagtagt agctagagat atttttgagc
    18301 ataaactaaa gcttataaac tctactgtaa atagattggg gcttaaaaat gtgtttgtag
    18361 aaagatttga tgcttctgag attgatgaaa atagtataag taaatttgat tatgttttag
    18421 cagatgttcc ttgctctggt tttggtataa taagacgtaa accagaaatt aagtataaga
    18481 aagaagaaga gttaaaagac atcacttcta tacaaaagaa aattttagaa aatgcatcta
    18541 aatatgtcaa agttggtgga actttagtat atagtacatg tacaattcaa gatgtggaga
    18601 atataaatat agttacttct tttgttgaag aaaatcacaa ctttgaattt gttccaattg
    18661 atacagtaaa tgtagactta gacaatcaag ataaaggata tttaaagata tatcctaata
    18721 ttcatggtat agatggtttt ttcatagcaa aattaaagag aataaggtag tgagagaatg
    18781 gaaaagaaaa agatagtatt aaaaaatttt actgaagatg aacttaaaga atttatgaaa
    18841 actatagatg aaaagccctt tagaggaagt caaatattct cttggatata taagggtgca
    18901 aaaacttttg atgatatgaa caatatacca aaaagtttga gaaataaatt agaagaggtt
    18961 tcatgtatag ggcatataga tatagaatta aagttagaat ctaaggtaga taatacaaaa
    19021 aaatatttgt ttttattaga tgatggaaat ataatagaaa ctgtgatgat ggattatgat
    19081 agtagagtta ctgtctgtgt ttctaaccaa gtaggttgta ggatgggatg taatttttgt
    19141 gcatctacaa tggatggatt aattagaaac ttagagccat gggaaatttt agaccaagtt
    19201 attaaaattc aagaagatac tggtaagagg gtatcaaatc ttgtattgat ggggagtggt
    19261 gagccacttg ataactttga aaatacaaaa caatttttaa aaataattaa cgaaaaaaat
    19321 ggtcttaata tagggtatag gcatataact ctttcaactt gtggaatagt acctaaaatg
    19381 tatgagttgg cagacttaga aatagctata aatttagctt tatcacttca ttcaccatat
    19441 gatgaagaac gaagaaaaat tatgcctgta gcaaatgctt attctattga agaaatattg
    19501 aatgcatgta gatattatat aaaaaagaca aatagaaggg ttacatttga atattctctt
    19561 ataaaagggg taaatgactc agaaaaagaa gcaaaggcat tagctaagct cctaaaaggt
    19621 atgctatgtc atgtaaattt aattccaata aataaagttg aagaaagaga atatgaaaag
    19681 ccagataaag catttattta taaatttagg gatagtttgg agaagaacaa tatacctgcc
    19741 acagttagga tgtctatggg ttctgatatt agtggagctt gtgggcaatt gagaagaaaa
    19801 tacaagtaat ttgtaaggag gaagtcgtat ggtttatagt tgtgcctccc atataggaaa
    19861 aataagaaaa aacaatgaag actattgtga gggagaagtt atagatacag aacatggtcc
    19921 aataggaata tttgccatag ctgatggaat gggtggacat aaaaaaggtg aagtcgccag
    19981 taaattagca gtagaaaata taattgattt tctaaaggaa aacttattac aacatgataa
    20041 tgtaaaaata gactatatag atgatatatt aaaacaggct tataataatg taaactcaat
    20101 tgtgcacaaa aaatctatgg aagatataga atttgaagga atgggaacta cattagttac
    20161 ggctatagtg tataataatg tcttatatgt ggcaaatgtt ggagatagca gatgttattt
    20221 gttaacagat gaaaaatttg ataaaataac tatagaccat tccgtggttg aagaacttat
    20281 gatggcccat gtcattacag aagaagaggc aagaagacat ccacagagga atagaataac
    20341 tagagcaatt ggaacagatg atatggttgt tgtagatatt tttaagaaag aaatcaaaaa
    20401 aagtgatata atacttcttg cgacagatgg attaactggg tttattgatg atgaagatat
    20461 aagagattta atattagatt atgaatatga gagaactagt aatataagtg aagaattgat
    20521 aagtatggcc aatgatgttt ctggtaaaga taatgtttcg gttatagtga taaaagtata
    20581 attaggacgg tgatattgtg ggagatacaa ttttaggaaa tcgatatgaa atcatcagga
    20641 agattggtga tggaggaatg gcatttgtat atgaggctaa agacagatta ttaaacagga
    20701 ctgttgcact taaagtactt agacctgaat ttgtagatga tgatgaattt ctaacaaagt
    20761 ttaaaagaga ggcagaagca gtagcaagtc tttcacatcc aaatatagta aatgtttatg
    20821 atgttggtga agatggaaaa gttcactaca ttgtaatgga gtttgtagat gggaagaatc
    20881 taaaagagat tatacaagat gaaggtatat tagatgaata tactgcatta gatataacaa
    20941 aacaaatagc tatggcactt agtgcagcgc acaaaaaggg gattatacat agagatataa
    21001 aacctcataa tattctcata tcaaatgaag gaagagtagt aaaagtggct gattttggta
    21061 tagccaaagc tgtttcaaat tcaacaatga ctaatattgg tagtataata ggttcagtgc
    21121 attatttttc accagagcaa gctaaaggta aatttgtaac taataatgct gacctgtatt
    21181 ctctaggtat agttttatat gaaatgttga taggaaaagt tccatttagg ggagatagtc
    21241 caatttctat agcacttcaa cacattaatg atgatataga ctttacatca gaagaaaaag
    21301 ttaggattcc tcaaagtgta agaactacaa taaaaaagct tacagaaaaa tctagtgcag
    21361 atagatatca aactgctgaa gaattgatag aagatataga gtatatagaa aaaaatattg
    21421 atttagattt tataaaagaa tatgatgatt ttgcaactaa gaaaattgat gaaaaagaaa
    21481 ttaataaagt tgtaaatcca acacttgcaa aaccagctcc tgaaaaagtg gttaagcctg
    21541 ttgaagtagc tgatttggat gatgatgaag attactatga tgatttttat gaagaagatg
    21601 atgatgaaga tgaagaagag gaagagataa tgagagctaa aaagaatcag agacctaaga
    21661 gtactccaag taagagaact aagaaaaaga agaaaaaaca ggaaagccca aaatctagaa
    21721 gaagattaaa agtaattgcg gctgttttga tactgatatt gtgtgctcaa gtattcttag
    21781 catataagtt tttatttgct ggagggtttg gcaataaatc tttgactgtt cctaatcttg
    21841 taaatatgac attagaagaa gctcaaagtg ctgttgaaaa agaaggatta tacctgagtg
    21901 taaaaagtga agagtataat agtgaagttg atgaaaattg tataatatca cagacacctg
    21961 aaggtggaag tacaaatgtc aaaaagggtg atactataaa tgttgtcgta agtaaaggtt
    22021 caagtcaggc atctgttcct aatgtagtgg gacttacttt gagtaatgca aaacaactta
    22081 ttgaagaaaa taacttaaaa gtgggaactg ttaagtatga atacagctca atatataaag
    22141 aaggtacagt tttaagtcag agtccaggtg ctggctcatc aagagctcag gaaggtgatg
    22201 aggtaaatct atatgtaagc aaaggttcag aaaaatcaaa tacacagact ccaacagtac
    22261 cagataaaaa accaacaaca ccagagaata atacaccaac agaacctggt tcaaactcag
    22321 gaaacagtgg tggaaattca ggaggctcaa attcaggaaa tagtggtgga aactcaggca
    22381 ataatggaag taactcagga ggttcaaact caggaaatag tggtggaaac tcaggaggct
    22441 caaactcagg agataatgga ggaagttcag gaggctcaaa ctcaggagac aatggaggaa
    22501 gttcaggagg ttcaaactca ggagacaatg gagatagttc aggaggctca aactcaggag
    22561 ataatggagg aacttcatca ggaactggcg caaacattgg taaaacagaa taaatattaa
    22621 taaaataaga gctgtccttt tatagaaaat attttcttta aagggcagtt tgttttgtat
    22681 aaaatactga attggttata atataaggag aaaagtattt atgaagaaga aaacagtttt
    22741 aattacagga ggagctagag ggattggtaa agctatgtct aaagcttttg caaaagaagg
    22801 ttacaatgta ttagtaaatt ttaataagtc agaaaatgaa gctaaagagt tgtacactat
    22861 attaaatgaa aaaaattttt ctgtaaaact ttttaaggct aatatttcaa atagagaaga
    22921 tgtagaggat atggtagatt attgtataaa agaatttgga ggattggatg tacttgtaaa
    22981 caatgctggt gtaagtcaag ataagttgtt tacagatata actgatgaag attgggataa
    23041 tatgatgaat attaatttaa aaggaagctt ttattgttca caagttgctt taaaatacat
    23101 gatatcagaa aaaaaaggaa atataattaa tatatcttca atctggggta tatcaggtgc
    23161 gtcttgtgaa gtgcattatt ctataacaaa agcaggtata ataggtatga caaaggcatt
    23221 agctaaagaa gtaggaccat caaatattag agtaaatagc atagctccag gtgtcattaa
    23281 tactgacatg ttatctggat ataatgagga agatatagac gctcttgtgg aagaaacccc
    23341 tctaatgagg ctaggaactc cagaagatat agcaaattgt gctattttct tggcatcaga
    23401 caaatcaaat tttatcactg gtcaggtaat tagtccaaac ggaggatttg ttatttaaaa
    23461 aaaatgtggt attatataaa aataggaaag aataacacga tatactactc ataaacagta
    23521 tgtgtttata taaattttat aaagggaggc atttgatgct agaaggaaaa attataaagg
    23581 ggattagtgg attttattat gtagatactt ataatggtat ttatgaatgt aaggctagag
    23641 ggatatttag aaagcagaaa ataacacctt tggttggtga tagagtcaaa ataagcattg
    23701 ttgatgaaga tgaaaaaaag ggtatcttag aagaaatcga cagtagagat acagaactta
    23761 taagacctcc tattgcaaat gtagataaag cattaatagt attcgcaata aaaaatccta
    23821 aaccaaactt atctctttta gatagattta tagttttggc agaaaaagaa aatttagaaa
    23881 ctgtaataat ccttacaaaa gcagatttag atgataatga tacattagaa actgttaaaa
    23941 atatatatga acttagtggt tataaagtca ttcctgtaag caatattact aagttaaata
    24001 tagataaagt aaaagaagaa ttaaaagaaa atgtagtagt gtttgcagga ccatctggag
    24061 ttggaaaatc aagcttatta aatgaaatag atgaaaattt taaacttcaa acaggagttg
    24121 taagtgataa aataaaaagg ggaaaacata ctactcgtca cgctgaactt ttaaaattag
    24181 aatttggtgg aatggttgca gatacaccag gttttagttc ccttgcactt gaagatatag
    24241 aggaagttga acttaaagat tattttatag aatttgataa atttaatgat tgtaagtttg
    24301 gttcaaagtg tattcatgaa aatgaaccta attgtgctat aaaagaagct gttacaaatg
    24361 gagaaatatc taaagaaaga tatgatagtt atatacagct gctacatgaa ataagacaaa
    24421 ataatagtag gaggtattag aaacatgatt aaattagcac catcaatatt atcagcagat
    24481 tttgcaaaat tattagaaga tgtaagaaaa gtagaaagtg caggatgtga atatcttcat
    24541 atcgatgtaa tggatggaca ctttgtacca aacattacat taggaccact tgtagtcaaa
    24601 agtttaaaga aagaaaatat aaatatggta tttgatgctc atcttatgat agaaaatcca
    24661 gaccagtata ttgaagaatt tgtaaaagca ggatgtgata taataactgt tcaccaggag
    24721 gcttgtgttc atctacatag aactatacaa aatataaaat cacatggaat aaaagctggt
    24781 gtagtactta atccagctac accagtagat acgattaaac atgtactatc tgatttagat
    24841 atggtattat taatgtctgt taaccctgga tttggaggac aatcatttat tccttgtgtt
    24901 ttagataaga ttaaagagtt aaaagctatt atagatagtc aaggattaaa cattgatata
    24961 gaagttgatg gtggtataag ccctaaaaac gtagccgaag ttgtacaagc aggagctaat
    25021 gtaatagtag caggttctgc aatatttgga agtgatgaca ttcaagaaac agttaattta
    25081 tttagaaaga atgcttctct tgaagaatta gtttaaattt aataatgaaa atctgtattg
    25141 ttttaaatgg cgaaattgaa gattacgaag ttacaagaga tattataatt aaggaatgtt
    25201 atgactgcat aatatgtgca gatggagggg caaatcacac ttataaaatg gaaataatgc
    25261 cagattatat attaggagat ttagattcag tagaagaaga aaagataaac ttctataaaa
    25321 ataaaggtgt taaatttgaa aaatttccat ctaaaaaaga tgagactgat acagagcttt
    25381 gtcttttttt agctaagaca cttaaagcaa atcatattga tttttttggt gctttagggg
    25441 gaaggattga ccacactctt gcaaatataa agctattata ttatttaaaa gaagatggta
    25501 tatattctag gatactttct gataaagaag agatgtatat agttgaaaat gaggaaattt
    25561 cattgtatgg aaacccaggg gacactattt ctgtaatagc tataaatggt gatgctaagg
    25621 gtgtaacact tactggctta gaataccctc tcgataatta ttatatgaaa tactctgtac
    25681 ctattggaat atcaaatgtt atgttgagta attcttgtaa gattaaagta gagcaaggct
    25741 gtgtacttgt agttagaaat ttataaaatt tgattttgac aattggtttt atattgtaga
    25801 gcagatacaa tcaagtaaaa tatatttatg attgtatctg ctttttagta tataattata
    25861 aatatataat aaactttgac ataaggggga ttttttgtgg tagatagtat tatttttgat
    25921 ttagatggaa cattgtggga ttcaactgaa gaagtatgca aagtctggca ggatgtttta
    25981 agtgagcata aggaaataga attaagtgtc acaaaagatt tatttagaag tcttatgggg
    26041 ctttcttttg aggaaatagc aaatcgttta tttccaaatt taagtgaaga agaaagaatg
    26101 aatttattga atgaatgttc tttgaaagaa tgtgactatt tatctgaaaa tggagctaaa
    26161 ttatttgatg atatagaaga tactcttaaa ttactatcta aaaaatataa attatttata
    26221 gtaagtaatt gtcaagctgg atatatagaa tcttttttaa aagcacataa actagaacag
    26281 tattttatag attttgaatg tcctggtaat actggtttgc ataaaggaga aaataataaa
    26341 cttataattg aaagaaataa attaataaat ccaatctatg ttggtgatac gcaaggtgat
    26401 gcaaatagtg ctaagtttgt aggtatacct tttatatatg ccaaatatgg gtttgggaat
    26461 gtagatgtat atgattattt tatagattca tttaaagatt tgttagaaca tgatatatta
    26521 aaataagatg agtttaaaaa ttatattaaa ataataaaaa agttatgggg ggatttaaaa
    26581 atggaattac aagatactat ttttaaaaga caaagtgtaa gaaagtttaa aaatcaagat
    26641 gtatcagatg aagatatttt aaaaatgata aaagcagcag gtgcagcacc atcaggcaag
    26701 aatattcaaa actggcattt tgttgttata aaacgccgtg atttaatgga aaagatagca
    26761 gatgtgataa ctaaaaagca acaagaaata cttgtagaaa tggataaagt atcagtggat
    26821 aaggctaata gatttagaaa atttgttaaa aattttacac tattttactt aaaagctcca
    26881 gttttagtat tagtttttac aaaggtatat aatccatcag gatattatga attagagctt
    26941 atagatgcac ctaaggagac tatagataaa ttatttataa gaaatccagg tatgcagagt
    27001 ctaggtgctg caattgaaaa ctttacacta tcagcaattg aacttggata tggttcttgt
    27061 tggttgacaa gtcaaaacta tgcagcagat gagatagaag ctgttttaga agcagaaact
    27121 ggttttgaaa agggagagta ttttttaggt gcaatgttag ctcttggagt gccagaggac
    27181 aatttgaaga gtccatctaa aaaaccagta gaagaaatat gtacatttat aaaataaaag
    27241 tcaaggacat tttaaaatcg aagtaaatta aaaacaaaat aaattaaaaa taaatattta
    27301 aaaaattaaa aagtagg
//
LOCUS       DAMJWU010000042        27248 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMJWU010000042 DAMJWU010000000
VERSION     DAMJWU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.042"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(<1..896)
                     /locus_tag="PC460_002004"
     CDS             complement(<1..896)
                     /locus_tag="PC460_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2600890.1"
                     /translation="MKLKHIAIIGSLFPILFSIVLFFGVLMSADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVNVLLAIIQVESGGTAKDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQMQYDATQHIPLSQA
                     KAGELVFFHSTYNAGSYVTHVG"
     gene            complement(893..3085)
                     /locus_tag="PC460_002005"
     CDS             complement(893..3085)
                     /locus_tag="PC460_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2600891.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPENEDGKTREEVVKTEIESNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVAGAVAGSTVSNARTTRNNSAKDNRGNTSSSMGQR
                     AGSKVGAVLDTKNKVKDKANAMKETVKDIPTQTAYAVHSAKEKAKTSVSDFKRGMVQE
                     QQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQARKTDGSATTGATRPHERPVTASA
                     IPKTNVEKTQEVKRPATTSTSKAGEPVKTNSVKERPLSSGTSDGKIPQSAQTVHSKNV
                     EKVVSHETHQNYKAERTTKAQTVEQAKRTTEHTEKNRNLVTKKGQKKK"
     gene            complement(3085..5535)
                     /locus_tag="PC460_002006"
     CDS             complement(3085..5535)
                     /locus_tag="PC460_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2600892.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNLKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEHQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRCEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGEILSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            complement(5522..5911)
                     /locus_tag="PC460_002007"
     CDS             complement(5522..5911)
                     /locus_tag="PC460_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2600893.1"
                     /translation="MKKIRSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPAFLTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLQKEIFNETITAVRSVSYVPD"
     gene            complement(6029..6532)
                     /locus_tag="PC460_002008"
     CDS             complement(6029..6532)
                     /locus_tag="PC460_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2600894.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIDELNEMYEMIEELPDYIVDCLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            complement(6535..7038)
                     /locus_tag="PC460_002009"
     CDS             complement(6535..7038)
                     /locus_tag="PC460_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2600895.1"
                     /translation="MQETRVLVETKRTTGEETTSYWFDLPIEVAEFEEKLGVSTESQD
                     YRIIEKLLPYADEVHEHTSVYQLNELDYMYRQLSSDMQEEYTMLHTVYENLEALYICR
                     NVITLYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFETYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(6957..7253)
                     /locus_tag="PC460_002010"
     CDS             complement(6957..7253)
                     /locus_tag="PC460_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2600896.1"
                     /translation="MNDIFRDMQAKVGCENISDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSCQTLKDVMKQQKGREEQCRKQGYWWKRKEQLAKKQHHTGSTCR"
     gene            complement(7350..7952)
                     /locus_tag="PC460_002011"
     CDS             complement(7350..7952)
                     /locus_tag="PC460_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2600897.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            complement(8037..8198)
                     /locus_tag="PC460_002012"
     CDS             complement(8037..8198)
                     /locus_tag="PC460_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600898.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALVAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            complement(8365..10275)
                     /gene="ltrA"
                     /locus_tag="PC460_002013"
     CDS             complement(8365..10275)
                     /gene="ltrA"
                     /locus_tag="PC460_002013"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDF2600899.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNHILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11017..11151)
                     /locus_tag="PC460_002014"
     CDS             complement(11017..11151)
                     /locus_tag="PC460_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2600900.1"
                     /translation="MWELLKDLLLVSFGMGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            complement(11159..12358)
                     /locus_tag="PC460_002015"
     CDS             complement(11159..12358)
                     /locus_tag="PC460_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2600901.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPNNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIGYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIRKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTQPEPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHYQIIKQQTATVEE
                     MVANVKE"
     gene            complement(12560..13954)
                     /locus_tag="PC460_002016"
     CDS             complement(12560..13954)
                     /locus_tag="PC460_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2600902.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNMKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFSSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            complement(14071..14556)
                     /locus_tag="PC460_002017"
     CDS             complement(14071..14556)
                     /locus_tag="PC460_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600903.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQAFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            complement(14654..15037)
                     /locus_tag="PC460_002018"
     CDS             complement(14654..15037)
                     /locus_tag="PC460_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2600904.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITRRT
                     YDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIKAD
                     DIILKNKGGQVGNPQNHAPQGQPKK"
     gene            complement(15052..15378)
                     /locus_tag="PC460_002019"
     CDS             complement(15052..15378)
                     /locus_tag="PC460_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2600905.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFTPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            complement(15614..18658)
                     /locus_tag="PC460_002020"
     CDS             complement(15614..18658)
                     /locus_tag="PC460_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2600906.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTDNHNGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITNGNG
                     NYSIAGATYGVFSDKDCTKQIATLTTDDSGNTEVAEVKATTVYIKELSAPLGYKVDTT
                     VYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTTAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAFLPL
                     GTLTVEETKSPTGYLLDGAYMQANGSEEQIKALYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETKDTKPQGSATLKDTAFDIISLNDNAVLVEGKIYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSKWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGMELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAEIIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPMKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DGKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETPTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            19186..20193
                     /gene="asrA"
                     /locus_tag="PC460_002021"
     CDS             19186..20193
                     /gene="asrA"
                     /locus_tag="PC460_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2600907.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKDVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            20194..20985
                     /gene="asrB"
                     /locus_tag="PC460_002022"
     CDS             20194..20985
                     /gene="asrB"
                     /locus_tag="PC460_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2600908.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            20998..21969
                     /gene="asrC"
                     /locus_tag="PC460_002023"
     CDS             20998..21969
                     /gene="asrC"
                     /locus_tag="PC460_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2600909.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            22026..22802
                     /locus_tag="PC460_002024"
     CDS             22026..22802
                     /locus_tag="PC460_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2600910.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            23073..25091
                     /locus_tag="PC460_002025"
     CDS             23073..25091
                     /locus_tag="PC460_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2600911.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            25306..26163
                     /locus_tag="PC460_002026"
     CDS             25306..26163
                     /locus_tag="PC460_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2600912.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            26260..27129
                     /locus_tag="PC460_002027"
     CDS             26260..27129
                     /locus_tag="PC460_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2600913.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         8252 a         5026 c         5015 g         8955 t
ORIGIN      
        1 attcccacat gggtaacata cgaaccagcg ttataggttg aatggaaaaa gaccaactcc
       61 ccagcttttg cctgcgataa agggatatgc tgggttgcgt catattgcat ttgtgccgtt
      121 ctcggtaaag agataccagc ttttccataa caccattgcg tcagtcccga acagtcaaag
      181 gaagtgttgg gattgctacc gccatacaca tatttccagc cttgatattt caacgcttca
      241 ttcattacct tttgtgccag ttctcccgat acctgcggaa cagttaaata ctgattgact
      301 aattccacat aaaacatatt tccatagcca taccgccagc ccccattttt cgcaacggct
      361 atcgggttgg tgtaggttac ttttttaccg ccagatttct cacgggcaaa gctctctgca
      421 aggttaaaag tatgtttctt tccttttcct gccacatatc ccacatagcc accgccatag
      481 ttataggact gtatcgccac attcaaatca tttattcctt gatttttgca ggaagaaagc
      541 agggacgcaa aatatttaca cccctgcttg attgagcttt ctgtatctaa cgagttgggc
      601 ggtaaaccaa gactttctga actctgcatg acatcttttg ccgtaccgcc actttctact
      661 tgaatgatag ccagtaacac attcacatac tcagaaatac cgtattcttt agcgtacttt
      721 tccaccatag gctgatgttt caagacttct gcggataaat tcatacccgt aatgcccgaa
      781 gaaaagttgc tgttctcgtc gtcgctgtcc gcactcatca gtaccccaaa gaaaaggact
      841 atggaaaaga ggataggaaa cagactgcca atgatagcga tatgtttcaa tttcattttt
      901 tcttctgtcc tttcttcgtt acaaggttac ggttcttttc agtatgttct gttgtacgct
      961 ttgcttgttc aactgtttgt gcctttgtgg tacgttctgc tttgtaattc tggtgtgttt
     1021 catgcgatac cactttttct acatttttac tatgtactgt ctgtgccgat tgtggaattt
     1081 ttccgtcaga tgtacccgaa gataagggac gttctttcac actatttgtt ttgactggct
     1141 cgcctgcttt ggaagttgaa gttgtagcag gacgtttgac ttcctgtgtt ttctcaacat
     1201 ttgtttttgg aatggctgaa gctgtgactg gtcgctcatg gggacgggta gctcccgttg
     1261 tcgctgatcc gtccgtcttt cgtgcctgcc ttgcttcttg tgctttttga agttccatac
     1321 gcttgtcagc gatattcttc ttatgctgtt cctgcttttc caagcgtccc gactgtctgg
     1381 attgttgttc ctgcaccatg ccacgcttga agtccgatac acttgttttt gctttttcct
     1441 ttgcggaatg gacggcataa gcggtctgtg tcggtatatc cttgaccgtt tctttcatag
     1501 cgtttgcctt gtctttgacc ttattcttcg tatctaagac agcacccacc tttgaaccag
     1561 cacgttgtcc catgctggaa gatgtattcc ctcggttgtc ttttgctgaa ttattccttg
     1621 ttgtccttgc attagaaacc gtactgccag ccaccgcacc agcaacaccg ccagcggtaa
     1681 cagcaccagc aagccgtctt tccatacgcc tagccctatg tgccatataa agatacggtc
     1741 tgcggaaaat tcttcgtccc atgctttgac tgtcgttagc gttcaaagag aacatactca
     1801 ttaaatcgcc taacttcatg tagataccag cgaaacacac tatctgcaag aacgccacca
     1861 taaagaacgg gtagtctgtg gaaatgttat aaaacatact ggaaatgcta aatgccaccg
     1921 ttacaatgag cgtaattcct gcccttgtca ttatggtatt aaataccctc acgattgcct
     1981 gctttgccat gctttcatag ctcggtatca tggatagtaa aaagctgata ggtaaaaaca
     2041 tggcgaagat aataaaaagt atctggctga acaacatcat acccgtaagc aagaatacaa
     2101 atatcgtgat acctaagttg aagaacaaga ggaagaacac catacctaaa cggtttacga
     2161 cttgcggtat cgtcagattg ttgttgtcgt tactttcgat ttctgttttc acgacttcct
     2221 ctctggtttt gccgtcctcg ttctctgggc ttgccgatac aagagcttct acacggtctg
     2281 tcccgatttc ctctgcgttg ctgttcccaa actgtaagag tagccacggc tgttccactt
     2341 gaatagagaa taggctgtcc cttatcaagt ccacgctgtc cttgccctcg ctgtcagagt
     2401 ttgggagcat gatttttgtt cccaagtcca aagaagcggt actgatgtcg cttgaaaatt
     2461 catttatctt tttgatgtag tcgggagcat aggcaataaa tgaagccgac aaaataaaca
     2521 ccacaacaaa gttgataacg gcgtgaagtg ccttgctggt ttctctttta atcagtcccg
     2581 tataggcaac atatagtccc accactaaga taatgagaag caggaaaccg acatagaagc
     2641 cagaggttga aaaaccgctt gaggtaacgc ctgcaagggt ctgtatgctc tgcccgatac
     2701 tgtctgccat atcattgatg aagtccagct tgtaggcttc ctgcactaca tagcccgtgg
     2761 cattacttaa atataaactg atagtccaga caaaattagt gatacagtac aaaccatatt
     2821 gtactgattt tccaatcccg tccagccagt tccacggcaa ccaagcccag ctattatcca
     2881 catagaagtc aagctgatag ttggaaagcg gatatttgga atatagattt tctgcgttta
     2941 tcgtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa
     3001 gggagagtgc caccacgaac agagccattt tgagaaagtg gaaaatcttc ttttttgtga
     3061 acgcactctg tatcctttct ttcatcactc cacctcattt ctctgtatag gcggtctggt
     3121 atcaaaggcg tgtagcagtt cttcaaagac ggggtgtatc tgtaccacgc cgacacgccc
     3181 gtataagtcc tgcaacaagc attgtccgtt ctccaaatca cgaagtcgct tctgattgtt
     3241 ttcgtcctct ttgtcgatac cgaaaaattc taaagtctgt tttatctcgt tgatgtcagt
     3301 agaacggaaa gcgaatttta agccgatatt gtttttcaga ctttcctttg acacgtcccc
     3361 agaagattgc gtaacgaaat acacacctgc ctgcatagct cgtccagcac gcaccagctt
     3421 gttagataaa atttcgcctt gtgccacatt taagaacgcc cacgcttcat caaggtctac
     3481 gattttgaag atagagcggt cgctgtggat aaaatctaag gcaaaggtac taatcacaat
     3541 cagcatggaa accgatagta attcaatggt cgtgtattcc tcaaaagtcg tgtccttatc
     3601 tggcagtaca aggtcggcta cttgaatgat attgagctga ttatctaaac tgatagcatt
     3661 ttccaccgtt ccgtctgaaa acagaagatg tgcaaaatca tagtctgtaa agctgtcgat
     3721 atggtctgcg atattgcgtg aaattgcggt atcttcacag cgtaactcgt ctatcacatg
     3781 aagcaagccc cttgtttcac tctgggtaac agaacgcacc gccttacgaa gtacggggaa
     3841 tttttcgccg tccctagagg aaataccagt taaaaaggtt agtatgtcga ttgccagact
     3901 ttcagcgtct ttcacattct tcataatcac aaacgggtca agaagccctg cgttgtcctt
     3961 gtcgcttgtt aggtttacga tattgatttc atgggcgatt tctggaagtg tttctttcca
     4021 gtttccacgc tctgattttg ggtctaagat aaccgcttga ccgccaaaca aaacagaata
     4081 gtacacaagc aggttattac agaatgactt cccaccgcca agcgaaccaa caaaagcaga
     4141 agcaagggca tttgtaactg taccttttac gccttgactg gcaagggacg gttgcagata
     4201 tacatttctt cccgtatcaa cggaatagcc catatagata cccgtgtttt cccctaactg
     4261 ctgtgtcgca ccaaaaccaa gcccagctaa gaagtctgat tttacatact gcacataatc
     4321 attgatatat cgcttgctgg ctggtagaaa ttcagaatga agccccagca tatccccagc
     4381 aggacgtact aacttcacat tcaaatcgtc ataaaaatct ttgacttcat cacaacggcg
     4441 tttcagttcg tccaaatcat cagcagacac acggataaca taagagagct tatacatact
     4501 ttcttttgtc tggtctaaat cggtttccag ctcgtccacg ctgtctaaag cgtctactac
     4561 atttgagctg gtttcacttc cagcttgata agcgtgatta tcaaggtctt tcagttcctt
     4621 tttcttgttg cggacggtcg ttaatgcttt tcggttttcc acgatttcta cattcatgct
     4681 cgtatcaacg ggaaaggtaa attgctgttg ctggaaatag aagatttcac tagacggaaa
     4741 atcaagctcg cctacaatcg cattgacggt aaaataggac acataacttt ccttgtcctc
     4801 atgttccaat cgtaaatatc gctggctttc ctcaatcaca caccttgtcg ggcggataag
     4861 gtcgtaatac ttgataagtg tttctttctt atactttttc tttggtagct ggtgctcata
     4921 atcttcatag gcgataccgt ccctgccgta aagatgttcc ataagatacc caaagtcatt
     4981 gatttccaga cggcgaacct taaagcgacg ggagatttta ttttccagta acttttccat
     5041 tttcatgtaa cggttgattt catcattcgg catggaaaca aagtcgttca tcagcgtgtg
     5101 gttcacttca tggagaaact ctttgaacgt cagccacgcc gattttttaa ggttcttcag
     5161 attgagctgt tcttccgtaa ccatgagttt aaagccaaga aaaaagcggt agtccacttg
     5221 attgtcccca atcattgata ctaacgcttc ggtctgttcg tctatcttct ggtaagcgac
     5281 ttcctttaat ttccccgtaa ccaatctttt tgactgctcc tgcatacttc ttatagagct
     5341 ttccgtggca atctgcaaag catgaatttt accctcacgg gactgtgcga ttagctgtcg
     5401 gaaactgtca tgcacgataa atttctgttc tgcggataag aaagaatagt tatacggtat
     5461 cagctcatag taggcgaaca cctcattgtc cttgttccag acaaggttgt tgtcaatata
     5521 tttaatcggg aacataactc acactcctta ctgccgtgat tgtttcgttg aatatttctt
     5581 tttgcagttt tacggctttt cctgcataag tgattttcgg tcgcaacgca taggttatct
     5641 gtgatttcaa aaagctgtat ggcttctttc cgtcaaaggt tttctgcgac ataaaccaag
     5701 tgaggaaagc aggaataccg aagtatttta aaaatgctcc ctcaatcatg gaaagtgggg
     5761 gcatatcccc aaacagaatg atgataaatt ctgtaatcac aaaccatgta atctgtgtaa
     5821 aggtaacggg aaagggcaag ttaaagtcat tgattgcata caagactttt tctacattcc
     5881 agatacccgt gtagcttcta atctttttca agttctttca actcctctca taagtttcta
     5941 aatggaaaag gacagccctg tttcagactg tcccttaaag agaaatacgc cgtcagtagc
     6001 aacaatgctt gtcgctgatc ttccagcgtt aatatggtat ctcgcacatt cctcggcttg
     6061 ttgcaatgaa gcaaccgccc atatccaaat ctcgcccgta tgcttcatag tcaatatagt
     6121 tctgcaagct agggggaata tcgccaagtg cctgcaattc atctatgtag taataggcaa
     6181 catctgtcat ggtttcacag tcgggataat agtaaatatc gtccttgtgt tccacgactt
     6241 cctccagcgt cccataatga ccaataaatt cgtccaaaca atccacgata tagtcgggaa
     6301 gttcctctat catttcatac atttcattga gttcatcaat ggaaacatac tccccaatct
     6361 caatcgggaa gttatcggta tcatggattg cgtattcttc atactgttca ttcaagccga
     6421 ttttttcttt cacatcttcc tcgtcaatag ggaaagtaaa ccaagcaccg actaaatagc
     6481 cctcattata cttgccaaga ttagcgatat aaactgccat atcatcaatc atacttactc
     6541 tggcaattca aagataccgt gttccgttac taaaaatttc ccgtgttcct gcaagtgtga
     6601 agcataggtt tcaaagtcaa aatagtattc ttgacagtcc tcggataaat gtttgaatgt
     6661 agggtcattc atcagctttt gccttgcaac atcaatcatg cttttacagt cgggataaag
     6721 cgtaattaca ttcctgcaaa tataaagtgc ttctaaattt tcatacaccg tatgaagcat
     6781 tgtatattct tcctgcatat cgcttgaaag ctggcgatac atataatcta attcattgag
     6841 ctggtacacg cttgtatgct catggacttc atcagcataa ggcaacaatt tttcaataat
     6901 gcggtaatct tgactttctg tactgacacc taacttttct tcaaactcgg caacctctat
     6961 cggcaggtcg aaccagtatg atgttgtttc ttcgccagtt gttcttttcg tttccaccag
     7021 tacccttgtt tcctgcattg ttcctcacgt cctttctgtt gtttcatcac atcttttaaa
     7081 gtctggcatg acataccaaa cacatatttt gaaaaatctt ctaactgttt ttcttcatag
     7141 tcggcaaggt tcagttgctt catttcatgc cacaccttac gcttgtaaga gggcaggtct
     7201 gaaatgtttt cacaaccaac ttttgcctgc atatctctaa aaatatcgtt cattacatca
     7261 ctccaatctg aaatttaggt aagaaaaaag cagtcaatcc taagactaac tgctttgtgt
     7321 gtatggatat gaagttgtca aactgggatt tatctggcag acttgtttat ccactcatca
     7381 agaaatttca tttgttcttc cgtgtgaaac caatgctctc cgtttctcat aacagtaagt
     7441 gttgcattag tttgttttgt aaattcagat atagttgcat aagaagtcaa gttatctttc
     7501 tcaccaaata aaatatttgt tggaattttc catgtagttg gattttcttt tacataacag
     7561 agatattccc atgagagtgt ttctccaaaa gttgttttaa tttctttttt atctcgaagt
     7621 tcatcttctg taacatttgc ccaatacatc atatctgcaa tcagtttttc catattcaca
     7681 ataggtgaaa taaaatatgc tttttctatt tgcttatctg ataaggcgtt catagcgaaa
     7741 taggcaccta tgctatttgc aactatttga acagatttgt aatgtttgca aatcaaatca
     7801 aaaagtgatg gaaattcttc ttttgcttcc catggatatt gtgcagtata gttgagtcca
     7861 accacatcac aatcactaaa aagaggtttg taatgtgaag actcctctgc atttccacct
     7921 ttaccatgta tataaataac tactttatcc ataatagttg tcctcctcaa cttcaaattt
     7981 gtcgctttct tcatatccac attatatcat tctcatatca gcaccacaat aaaattttac
     8041 gcaccgataa tcttattgaa cagctctaac agtacgtctt tcacaccgcc agcattgaat
     8101 accaatccaa cggcaacaag ggcaactacc aagaagccga taagtttaga aaactcacgt
     8161 ttgaacccta agtagatacc aataaccaca atcgccataa gcactaagct ctgggcgttg
     8221 cttaaaaacc atgtgtatat ggataggtaa gttcctgata ttataaatat ctcaaacttt
     8281 tccccccatt cacacggagc gtgcgacttt caacgcactc cgcgttccat cgtgtttata
     8341 ttgaaaagta tttatgtagt agacctatat caagtttaat tcttgattgc cagctaatac
     8401 tctatattta tttagtctta tgagctggac tttatttagc ttcaatagag ctatatattt
     8461 ttgtattgtt tcttctgttt tagcgtgtat tagacggtgt accgcctctt ttaccaaaat
     8521 atgattttta taatcgtcat ttccaccagc tccagttggt acaatatgat gacaatgtat
     8581 ttcattagct tgttgaaatt cttccagagt tatagcacat tttccatgtt gagcagaaaa
     8641 tagcgaaatt cgattgtctg cgtattctat tgtgcgattt tccaaagata atttcattaa
     8701 ctggtacatc agtaatttat caacacttag attggtgtgg ataaattttc ttccctctag
     8761 agtgtagcaa ttcgctaaat acacctttgc cattggtttt ctgtgttgga tatatccaac
     8821 agggaaaata ggttcgccct cgccagcaac ataccggagc attttagatt taccatatct
     8881 tgttgcctcc tttgtcgtaa gttgtctgcc tgtttttctc aatctgcccc ttttgtttgt
     8941 acctaatctg ttagtgaaga tagccattac agctctattc agtttcatac aatccagatt
     9001 tacattcgtt gcaatctgat aataattttg catacctaat accattgaat tataaagtct
     9061 tgtttcttct aattccgttt tcccttttga gggagaagca attttctttg cctgtgatac
     9121 cagcttgtct ttttggtttt tgaattgttt atcacttata tgtgatttaa caactaactt
     9181 ctttcttctc ggtatcattt taatcttgaa accaagaaag tcggagtatc tttttctaac
     9241 atttacaatt cttgtttttt cttgtgatac ttccaatttt aatcgttgtt caatccattg
     9301 agtgattgct attttggctt tttcagcact ttccttgtag cggcagaaaa ctctaaaatc
     9361 atctgcataa cgaaccatat acatttcctt taactttgtt ttcttcattt ctttgtaagc
     9421 gttgctttta catgggctac cgcttttatt gatatgaacc acatagtttt ttgtaacagg
     9481 attttcctgc cattggcttt ctacccaatg gtctaactca ttaagaacaa tgtttgctag
     9541 tagtggggat attattcccc cttgcggagt tcctttctct ggatatgtta ttgtaccgtt
     9601 agtcattttg attggagctt ttaatatcct ttttaaaatg aatatcaagt gcttgtcccg
     9661 tattcccata gcccatatct gttttatcag cttggaatgg ttcacgttgt caaagaaacc
     9721 cttaatatca aattcaatca cataatgtag ctggctgatt tgtaggcggt tataggttgc
     9781 ttttattgca ttttcaactg acctattagg tctgaaacca tagctgtttt cactaaattt
     9841 agcttcgcag attggttcta atacttgttt tatacattgt tgtataagcc tgtcccacat
     9901 acaaggaata cctagaggtc tggttttacc atttggtttt ggtatttctt ttcgcctcac
     9961 tggcttaggt cgatacccat gtggactgcc tgcaacaatg tatcttactt ttttgacaac
    10021 tgtttctgct ggcaatttgc caatatcttt aattattgta ccgtctgttc ctgctgtttt
    10081 actgcctgca tttgctttga tatttcgata tgccagtaat atattttctc tcgataggat
    10141 aatatccata agatgagtga aaatatctcc attcttactt ttggcatata aatcatcaaa
    10201 ggtgttttgc atttcgtaat attcagcgtg tcttaaatct tctacacaca atgtttcatt
    10261 tttattttta ggcataaggc ttcactcctt tgcttgaaat tgagtgagct tttttatctt
    10321 actcgtaatc cttattgaaa caacataata cttttctaat atatacacga cttgagggca
    10381 ttcctccatt ctcattacaa gaatttctta ggttcgccct ctgcttttca gcattagttc
    10441 aactgcttat tgttcttcgt cagtttattc tcttgatgag aacccaatac ctttcctcgt
    10501 tccgataatc ttatctatac atatatatcc ttaggtcact agctataagc ctgctggctt
    10561 gataccgcct gtaacgttat atggcatttc ataacctata ttcttacttt gccacaccag
    10621 ctacgatttc tcgtaccttt gcatttctac aaagttcaaa tttagacccg tacattcgct
    10681 gtttcgtcag ttccttatag aacattctaa ccatagatat tttatagacc cccgacctgt
    10741 tggatacgct acccgcctct ggatagtttt cgatagaaga tatatgttca tcaactatta
    10801 cggtatcttt cagccgtctt taccgagctt caaacattct gagtttcctc atacaatgct
    10861 tgtcggaggc tcagggcagg cgtttcagag cgttacttca tcattccacc tgtcagatta
    10921 tcagttctcc taataaccaa actttttcgt tcttttatta gtgccttagc ttttcactaa
    10981 ggaacgagtc gcacataaat tttgtccgaa attcatttaa ttgtcctctc tttctttgat
    11041 ttcttccata catctgtcag cttctttgcc gacagttaaa atgcacatca agactacacc
    11101 gatacccatt ccaaatgata ccagcaataa gtctttcaat aattcccaca ttttttcatc
    11161 actcctttac gttggcaacc atttcttcaa ccgtggctgt ctgttgcttg attatctggt
    11221 agtgtcgttc ggtcagtttt gcctgctttt caatcgtttc cagatactcc gtctgattgt
    11281 ccttgtctat ctgcttcaac atcttcaagg tcggtgcaac ctgcctttgt agccagttga
    11341 gtgtacgctc cagcgtgtaa ggttctggct gtgttgtcag ctttacgggc ggtcgcccct
    11401 cgccaatgaa ccaagcccag cgtacattgg ttttccagtc gctttttcgc ttgtctggtt
    11461 ctttatcagc gaagcggata tattgattga taatagaaaa tgctgtcagt tctgcgtcat
    11521 aatgtgtcag cagttccaca acagcataat aggcacgttc atttttcaaa cgtatctcaa
    11581 atctattttt tatggggact tcctcaatcg gtatgccaag tttggcgtac tgttcgtaat
    11641 tcttttcata acagcagaag tacacttcac ttgaaaatga accaatatac agcgtgtgtc
    11701 ccataccgta tttatccgtt tcattatgct ttaccagctc tccagaacca tagcttttga
    11761 aagaacggaa tttagaaata cattcctcat tctcgcattt tcgtatcagc tctggaatat
    11821 caagtaagcc cgttcggtca ttgattgcca aatcaagacg cttcattaca ccgccctcaa
    11881 ccaatgcgtc cgtgaagaag tcataccaac ttcgctcttg tgccagcaga tagctttcaa
    11941 actggcgaca gcctttgcct ttcagttcca gcaggacacc tttttcttcg tcctgcgata
    12001 catacacaaa cacatctccg atatggtaat gctcggtgta tttgtaatgt ccgtaatctt
    12061 cgtgaagcat atagccgatg ttgagcttca atatatcctt gatgatatgc tttatatcca
    12121 ttgtgggaaa acggatacgc acataatcaa acagcaggaa catgggattg ttgggattga
    12181 atttttccac cgcttctaaa agcgtatgtt tcatgtcctc ggtcagcgtt acctttcctg
    12241 cttcgatacg gctcaaatgt tcacggctga tatttgccat gattgcaagc cttgtctgcg
    12301 atacgccgta tgcacagcgt ttttctttca aggcggttaa aaaatcagtt tcattcagtt
    12361 gtcatacccc caatcgtgat aattttgtga tattgtgata atttcaaggc tttatcacaa
    12421 aaggcgaaat gtacgaaaaa cccttatttc atgcggtctt gcaaaggttt ttgtggaagt
    12481 ttccgccgta attgtgcccc tctgttagat aatgaggggc ttgtatctgc tggcgtggct
    12541 aacgccacac cagcaacggc tagtccgtgc cgtcgccttt cgcttcgcac gtcgccgtcc
    12601 cgtcctgcct tgcgtatgca agtgagccta tggtctgcaa aaaatcatgt ccttttggaa
    12661 ctaagggtgt gtaaaattca cttatgacgc ttgttcccac atcacaatag ccacgtccct
    12721 tgatacgctt ctggaaaaac tgttttttca catcagaact gaacagcata ccgtaaccta
    12781 attcgctgat acgccccaag ccaacacgga aattaaaatt atctctgata ccgtccgaga
    12841 aatattttgc gtctggacgc tggcaggcaa caataaggaa atatcctgct tgtcgtccta
    12901 acatgacgat tttctttagc tggctaagta ggctcacact ttcttttgtc cccagcattt
    12961 caaaaaacgc cacatattca tcaaagataa gaaagcaggg cggtaaacca agataggcat
    13021 agttttcgcc cgttttatag tctgggtgtc gcttcatttc ttcgcttctt tgtaccatgc
    13081 cctcataaaa ggcattgaca caatcaatca tttcttcttt ggtgtgatac acatttccca
    13141 ttaccgtacc taaatctgca aggtcagcgt tcttcgggtc taatatgtaa agaacagcgt
    13201 tcgtatgtaa cagagcttca ataagcgtca gcagaaaata cgttttaccg ccaccagtcc
    13261 caccagcaat cagagcgtga gggagtgcgt catattccca tacaagattt ttcatcagcc
    13321 taagacagcc gttttctgct ttcacttcat caatggtaat gcggttcgct atcatatcat
    13381 aaagcatggt gtattcgata taaccgtcat gtaacgtctt gtcggtcagc tcacaataca
    13441 agccactttc caatttatcc tctaaccgta agagctggtc ttgatatttt cctaatgtaa
    13501 tttcacagcg gatatgaagc agtccttttt ccatttgata ataaatcttt ggaaaccaga
    13561 tgattttttc ccttgatctg ctttgcaggt cagtaaaaaa accgctgtct tgtacggtgt
    13621 ctgcttcata ccacttgttt tccagtatca ttcgtgccag cttttgacgg tgcaggagct
    13681 tcttgaaact gtcataacag aagcggtaat acagaaaagc gaccaatgca caaacaccag
    13741 tcgctatcag tagggttatg aagttgtaag tggaaagcgt caaaccgttg tctaataagc
    13801 tgaaatgctc ccaatcggta cgcatgagct gtttcatatt cagtagcagg ataaccgcaa
    13861 tgaatacaaa cagaagcgtc cctatggaaa agtggtagac aaggttcttg tcgctggctc
    13921 tgatacggtg tcctttgttt aaaatcattt tcataaatca atcactcctt tctgggtacg
    13981 aaaaaagcag tcaatcctaa gactaactgc tttgtgtgta tggatatgaa gttgtcaaac
    14041 tgggatttat tgttcttaac ttttctgtcg ttataaagct gacttacgca cattaacctc
    14101 tgcacagacc agcccagcta tatgctccgc tgtttcattt gtcagtacaa agagcattcc
    14161 gtcattacgg cattttttat gtgtgtcgcc attcaaggca taaacaaaac cttttacaca
    14221 ggtatcacta caggcattag ggtcaatcag tctcttgcag tcacgagaag tagtcatttt
    14281 cttttgcata tcagcaggaa gcgaagcgat tatatcctca tactttccag cgttgtctcc
    14341 gtaaatccgt gcaaaaacac ctgtcttgcg aaacacccaa ttcattactg ttttcttctc
    14401 tagttggtat gaagcggtgt aaccgctttt tgcttccttt attacaaggt cacagccctg
    14461 ctcaataagt ttggtattaa gtttctcgac aaacgcttgg tgttctggcg cgattgccga
    14521 tagaaattct ttgaatgaac ctttttcttt tgccattatc tttttcctcc acaaatttca
    14581 atttatccgt ttcttcatac acatattata acagttgctt atatccgcta caatgaaaat
    14641 ctagtttgcc agcttatttc tttggttgcc cctgcggtgc atggttctgt ggattgccta
    14701 cttgaccgcc tttattcttc aagataatat cgtcagcctt gatataccag tccacattga
    14761 cacctcgata gctggcgttt gctaccgtat cggctacggg attgacaagc tctacctctg
    14821 cattatacgg atagtccttg acgggaatat cagcaggaat tgatacttgt atcatacgtt
    14881 cctgcacact acatttcaaa tcataggttc ttcgtgtgat ttcttcactt gttgaaccgt
    14941 cgtcattttc tgctcgcacc tcatgtctga gggcagagaa cttcaataca ccgaaagttt
    15001 tttccttgtc taatacaata ccgtttgcta atctcataat catttacccc cttacttgct
    15061 ttcctcggct ggcaacatat catcagccgt taagatatag tttgtgaacc ctcgtccttt
    15121 gaccgcttta ccgtctgcgg taatctttgg attgataagt tttactcgct gttctggttt
    15181 gaaatgcttt tcgcctgctt tcgctggaat ggttacgata atatcatcag ccctttgtac
    15241 gtcagagtac aagttgtagc ttcttgatac gggcgtaaag cgtccgttaa tgcgttgctg
    15301 ttcaatcttg ttttcgccag caaattccaa gtttccaaaa gtctgtgcca tgttcggcac
    15361 tacatatttt aattccatag tgttttacct cgtttctttc ttctttttta agtttgttga
    15421 ttgtgctttc ttattctggg aattagggaa ataccagtaa tcccccagat agtaaagggt
    15481 aaatcaatgc ttcgcaccct tgactatccg tgtgcttgca ggttgttggc agacaagcca
    15541 gaataagtaa acgtctgccg tttgacagct tcggcagaga gcgtgatttt tcttttctca
    15601 taccgttacc gccttatgat ttcttcattt tacggcgttt gtaaagatat gttccagcca
    15661 gtccaccagc agatgtaagc aacattacca gaaatgccat gacatttgtg ttgtcgcccg
    15721 ttttaggact gtctgttggt ctgctcggtt tttctggtgt tgttggcgtt tcgggtttct
    15781 ctgggacttc tggtttttcc ttaaaagtaa tcgtctgtcc ctcgtcctca atgtccttat
    15841 gctcagtaac ttttttcggc tcgtttggat tgcttaaatc atacaattct tcaaaagtta
    15901 caagctgttt gccgtcaaga gaagtagcgt caaaggtaaa ggtaacttcc actttcatag
    15961 tttcgctgtc agcgataaac gtataatcac tttccacacg ttttccattt ataagaagct
    16021 ctgcattttc ttctttcaac atctgccagc ccacaagttt atactttgta ccaacttcca
    16081 agccctctaa ggttacagta tcaatgattg ttacgtcttt tcctgcttca agctctttgt
    16141 taccgtcctt atcggtagca gttgtatgta ttttgatgat acgttctgta ataagtaccg
    16201 tctgcccgtc gtcctcaata tctttatgct ccgcaacttt catgggttcg tctggattgc
    16261 ttaagtcgta taattcctcg aatgttacca gatttttacc gccaagctcg gacgcattga
    16321 atgtataaga aatttccact ttcatttctt catcatcagc aacaaaggta taatcgcttt
    16381 ctacacgttt cccgtcaatg ataatctcgg cgttttcttc ctttaacatc tgccagcctt
    16441 ttaactgata cttagtgcct tttgtaagtc cgtctaattt gacagtatca acgattgtta
    16501 cctcttttcc tgcaaggatt gtcttttcgc cgtctttact ggttgctgtg gtatggatag
    16561 tgatttcctt ttcgtattca tcagtcaaag tccctaaatc aatcacaaga ttatttcttg
    16621 ataccacgat ttcaaatggt gggataagtt ccatgccttt gttgctgtca gagcgtaatt
    16681 cttcaataat gtaagtatcg taaggcaacg cacctttgct gtcgtctggt tcagaagttc
    16741 caaaccatac accgtcctcg cttgttttgc cagcgttggt attgtgcttg tgcgaagccc
    16801 attttgaaga agtggagaat tgcccattat cgtcagttac cacaacatga ctttcgcccg
    16861 ttgtcttgct tgtgatccta aagggaacat cagcaagacg cttgtgtgtg cctgcaccga
    16921 ttttcacgcc ctcaatatcc ccacgcttaa tctgattgta gatagagtgg gcttcgtctg
    16981 ttaagtccac gatttttccg ttttctgtga ttgtaaaatc aatcggcttc gcaccgtctg
    17041 tcaagtaacc gtttggagct tgtttctcaa cgatacggaa attcccgtaa ggcaaaaggt
    17101 cagcagaagt agaagcgata ccctcaatat ccgtatggat tgtttttaca acttcgtttt
    17161 tcttgtatat cttgccctca accaataccg cattatcatt taaggaaatg atgtcaaagg
    17221 cagtatcttt caaagtggca cttccttgtg gtttggtatc tttggtttct aaatctcgtt
    17281 tctgaatttt gacaccgcca cgaatgactt tatctgatac ggaaaactgg ttacttccag
    17341 ttaatacggc aaggtcgccg tcctcggtaa tctgcgtaag gtataaggct tttatctgtt
    17401 cttcactacc attcgcctgc atatatgcac cgtctagtaa gtagcccgtt ggcgatttcg
    17461 tttcttcgac cgttagtgta ccaagaggta aaaacgcttt tccgtcctgc atatagaagc
    17521 tatcgccaga taccttgtat gcgtctgcta attttgtgat gtaatgggtt gtcccgttac
    17581 tgtctgtttc agcggttgtc tttgtaaccc atgtacgagt agcttcggca ggaagattgt
    17641 ctttattgta gaagcctgca taatatttcc atgtaaactc cgccccctct aaagaagcgt
    17701 tcccttgtgg gttggctttc tgtgtttcca tgtcaatttt aaaaagctca atcaaagtat
    17761 cggtaacttt tggcgtatca gatactttca aagtcgctgt ctttccagct tcgactttta
    17821 aggaatacac cgtagtatca actttatacc ccaatggtgc agataattct ttgatatata
    17881 cggtagtagc tttgacttct gcaacctctg tatttccgct gtcgtcagtc gtaagggtag
    17941 caatctgttt ggtgcaatcc ttatcggaaa agacaccgta agtcgcacca gcgatagagt
    18001 aatttccgtt accgttagta atgctggtat tattggaagt cttttgaagt ttggcatttc
    18061 cgacatttaa cttcgcccag aattgcccta attcctgccc ctcgccagag tagatataac
    18121 cgccgcattc atagcgtcct ttgttttctt tcacaaaggc ttttgcacca gcaaacactt
    18181 catcttgtgt tgcctttggt atttcatcat aagaagctcg cacgttatca cattgccagc
    18241 ctaagtgaac actcaatctt tgccacacaa caagctgtct taaaaggtag gcgtgatttt
    18301 tgcttatccc attgtggttg tcggtgtatt gcttcacata ttcgatagat aaggcaacat
    18361 cacttatctg gtcggcactc atacgggagc ttgcgtcagc tctggtcttg tagccgttct
    18421 taaaatctgt gttaatatcg atacaataag cgtcctcgcc ctcaacggtc aaatgcccct
    18481 cgttaaaggt agaaccaatc gaaccgtcat tcattacttt ttcaacgata ccgacacgtt
    18541 cttcactttc cgtccagtat tgcttgcttt ctgcatggac aagtgtagtc ggtaaagcag
    18601 taacgacagt cgcaaaggca agtacgcccg tacacaatcg tttcaatatc tttttcataa
    18661 ttctaaatgc tcctttcatt ttgggtaata aaatagccgt ccataatgaa cggctggaaa
    18721 tagaaaagga acgtcaattt aggcgttcca tagtctatga aatattcaat ttttttcttg
    18781 cttcaatact gatgtgccgt accatatctt tgcatatcta ggaaaacttg cgtatcaagg
    18841 ctcattcatt ggtagtaaat tagtagtaaa ttgatgtgtt tgtttccttg aaaatgctga
    18901 tagatactat gttttagcgg ataatcaact ttttagtatg ttttttgcat aaaaattgag
    18961 tagatagtag ttttattgta cttaatgatt gtatttaagt gattagattt gaaattgaat
    19021 aatgtagata ataaaaaaat attaacagaa cttaataaac tccattaata tttttttatt
    19081 atttttgaaa ctgtgataat tatcacggaa gcatagaaaa taaagtgtta agatttaaac
    19141 aagatagtaa gtaagtaaaa ctaaaatata agaaaggtga taaaaatgaa actaaaatta
    19201 gattcatcta agttcaatga aggtctgaat tatctaaaaa aagactacaa gatatttgca
    19261 ccagttttaa taccatttaa aggtactttt tcagatactg atgttataag atacaaggag
    19321 gtaaacgctt ttgaagaaat ggaatttgct aagaaagcaa acttttcacc aaaagatgtt
    19381 gtacttccca taaatcaagt attgttttat tttactgaaa aagagtttaa ggaaagcgat
    19441 ttagacaaca agaaaatttt aattttctta agagcttgtg acataaatgg tataaaaaga
    19501 cttgatgaaa tatatctaaa taatggtgaa gaaaaagatt atttttataa aaatatcaga
    19561 gataaagtaa agtttgcact tgtagggtgt aaggaaagtt ttagaaactg tttttgtgtg
    19621 agcatggatt ctaacaaaac tgataattat tcaattggat taaatataga aggagataca
    19681 ctgtacttag atataaagga tagtgaattt gaagtattta atggagaagc aactgaattt
    19741 aatgttgatt atgtaactga taatttaata tctgtagatg tgccagagaa tattgattca
    19801 aatgaaatga taggaaattc aatatgggat gaatatgata caagatgtat aggatgtggt
    19861 agatgtaatt ttgtatgtcc aacatgttca tgttttacaa tgcaggatat tttctataag
    19921 gaaaacgaaa atgtaggtga gagaagaagg gtttgggctt cttgtcaagt agatggatat
    19981 acagatatag caggtggaaa ttcttttaga aaaaaacaag gcgaaagaat gagatttaaa
    20041 actatgcata aaatacatga ttttaaaaaa agattcggat atcatatgtg tgttggctgt
    20101 ggaagatgtg atgatgcctg tccgcaatac atatcatttt caaagtgtat agaaaaaata
    20161 aatgatttag taattagtaa ggaggaagta taaatgaacc catatatacc agtaagtgca
    20221 aaaatactag atatagttaa acatactgat aaagaatgga cttttagagt aaactgcaat
    20281 acaaaaggtg tattgcctgg aaagttttat gagatatcta tacctaaata cggtgaaagt
    20341 ccaatatcag tatcaggata tggagaggat tatattgact ttacaataag aaatgtagga
    20401 aaagtaacta gcgagttgtt taattataaa gaaggagatt ctttttttat aagaggtcct
    20461 tatggaaatg gatttgatgt atccctttat gaaggaagag aaattgtagt agtggcagga
    20521 ggaagtgcat tagcccctgt aagaggaata gttgaatatt tttataataa taaagaaaaa
    20581 tgcgaaaagt ttaaattaat agtaggattt aaatcaccaa aagatatttt atttgcagat
    20641 gatttaaaga gatggagtaa aaaacttgat attttagtta ctgtagatgg agcagaggaa
    20701 ggatatagtg gtaatgtagg attagtaaca aaatacatac cagaattgga tatgaaagat
    20761 ataaacaata cttctatagt tgtagttgga cctccaatga tgatgaaatt tacagtagaa
    20821 gaatttttaa aaagagattt agatgaaaag aacatttggg tttcttatga aagaaaaatg
    20881 tgttgtggaa taggtaaatg tggacattgt aaaatggatg atacttacat atgtttagat
    20941 ggaccagtat ttgattattc atatgctaaa aatcttattg attagggggt gaagtttgtg
    21001 ataagagatt taaataccaa aaaagtaatg aaaaatgctt atagaattac aaaaacaaaa
    21061 tatgagactt ctttaagagt tagagtacct ggaggtctta ttgaccctga gagtttagct
    21121 gtagtttcta aaattgcaag tgagtacggc aatggacagg tgcacataac tacaagacag
    21181 gggtttgaaa tattaggaat aaatatggaa gatatggaag aaataaataa aattatacaa
    21241 cctgttattg aaaaaatgaa tataaatcaa agtgagaaaa acgcgggtta tccagcagca
    21301 ggtacaagaa atatagcagc ttgtatagga aataaagtct gtccaaaggc tcaatataat
    21361 acaactgaat ttgctaaaag aatagaaaaa gctatatttc caaatgattt acattttaaa
    21421 gtggctttaa ctggttgtcc aaatgattgt ataaaagcta gaatgaatga ctttggaatt
    21481 attggtatgg ctttaccaat atatgaaaag gatagatgtg taaattgtgg agcatgtgta
    21541 aaaaaatgtt ctaagatatc tgttggagca ttaaaaacag aaaataataa ggtggtaaga
    21601 gataaagata agtgtatagg ctgtggagag tgtgtactaa attgcccaac aaatgcatgg
    21661 actagagatg agaaaaaata ctatagatta gctataatgg gtagaacagg aaagaaaaac
    21721 cctagactag cagaagattt tcttttatgg gtagatgagg acagcataat aaagataata
    21781 cttaatactt atagatatgt agaaaagtat atagataaag atgcaccagg aggaaaagaa
    21841 catataggat atataattga tagaacagga tttatggaat ttaaaaaatg ggcacttgaa
    21901 ggtgtagagc ttccagaaat aactaaaatg tacgaaaaca tatactggag tggaattaaa
    21961 tatctataaa ttgagtaaaa catatatttg aataaattaa aatttaatag aaggaagagg
    22021 gagttatgca taaagaaact ttggataaat taactaatgc agctataaat aagataaatt
    22081 tattaaacac aagtaaagta aaatatttgg tatcttctgc gtttgcagga ctttatgtag
    22141 gtataggtat acttttgata tttactattg gaggactttt gactgatgca ggttcaccta
    22201 tgactaagat agtaatgggg ttatcatttg caatagcact aagtttggtt ataatgacgg
    22261 gaacagagtt atttactgga aacaatatgg ttatgtctgc tggtatgtta aataaaggtg
    22321 taagtataaa agatacttct aaaatatggg catacagttg ggtaggtaat ttaatcggag
    22381 ctttggtttt aggcattata tttgtaggaa ctggtctagt agacaaagga cctgtagctg
    22441 aattttttgc aaatacagca gctagtaaag catccatgcc atttacagct cttttcttta
    22501 gagggatttt atgtaatatt ctagtatgtg tatcagtgtt gtgttcgttt agaactaata
    22561 gtgatactgc aaaaataatt atgatattct tatgtttatt tgcttttata acatcaggtt
    22621 ttgagcatag tgtagcaaat atgacaattt atagtgtttc attattttca ccaacaataa
    22681 gtacagttac tattggagga gctatttata atttagtagc tgtaacactt ggaaatattg
    22741 ttggaggagc tttatttatg ggtcttggaa cttatatatt aggtaaagaa aagcttaatt
    22801 aaatttattg tataaattat aactctcttg ataaatacat ttttagtatt gttgagagag
    22861 ttatttttat atttttgaaa aaatatttga aacaaaaaaa atatgggagc atacgaaaat
    22921 atatcggtta tatatataaa aaatacgttt atatatgaat tatatagttc taaatataaa
    22981 aaaagttaaa gtcctaccgt aaaaaagcgc attttgaata agataacaaa tatgataaaa
    23041 ttggtcttgt tgaaattttt aggggggata tgatgacaaa gaaactagta atatcaaaaa
    23101 caaagttgtt tatttttata atagcattta tttctatatt tatatattta tttggaagta
    23161 aaaatacact tataggtgta ggtatagtaa cagctatgct aacactcttg gaaagagatt
    23221 taactatttc tccaattaaa aatttactga agtatttagc aataaatata atcttaggaa
    23281 tactatcatt ttttgcagta caaaatatgt atttaggagt tttattaaat tttatagcat
    23341 tatttataat agggtatgta tttagttatg acttaaaaag agcagtatat gtaccctttg
    23401 gtctaatgta tatatttatg gtaagtatac ctgtaggaaa aagtgaattt cctatgagat
    23461 tgtcggcttt agctgttgga gcagttataa taatgattgc tcaatttgtt atgaatagaa
    23521 ataggatgaa aaatgtcgga gataaagaac tgatatctat atgtgatgaa ttattagaaa
    23581 aaattagcct tttaaaaaat actattaatg atgatagttc tataattaaa tctagtatga
    23641 gaaaaataga tagctgtaat tatagaataa attctatatc taaaaatttg aaaatggtta
    23701 tttttgataa tagaaaagat gacttttata tcagtattag aggtatagac ataatgaata
    23761 ttttattttc attagaaaga ataagtttaa tcctggaaag atacaaaaag gatagtaaag
    23821 aatttgaaga tgaagatata aaaaatattt tagaagaaag tagcataaat agtaaatcag
    23881 atgttttagt tgtagcaacg aaagaaataa actatattaa gatgtgttta gaaaataaag
    23941 ataccataac tgataaagag atattaggat ttagagatta tgttattgct caggatacta
    24001 aaaatataaa tttaaaagaa atttatagtg ttttagaaaa tttatatgag tttttagtag
    24061 attataaaaa agtaaaatca agagacgaaa agaaagctga aagaaaatca aagatacctc
    24121 atgaatttaa acgactatca atatataaga aaaactttaa tttaaattct ataagatttt
    24181 cttatgcagt aaagattgct ttagctacag ctgtagcggg ttttattatg gattatttcc
    24241 atttaagaga tggaagatgg ataatgttaa ctgtcttttc attaacacaa ccttacgcag
    24301 aaaattgtat acaaaggtct agaaaaagaa tagaaggtac atttatagga gctgtaatat
    24361 ttattgtatt attttcaata ataaaagata gtacattaag gtcgttaata gttcttttag
    24421 cagggtatat aaactcttat gtagttgatt atagaaaatt aatggtatgt gtaactgttt
    24481 cagctcttgg ttcagcagta gttatgggag acccaaatgt tttaacaata agcaggattt
    24541 catatgtagc attaggagca attattgcat taatagtaaa taagtttata ttaccatatg
    24601 atgctaaaac aggctatcaa catgttatag aaatgtataa aggaatagtt aaaaatataa
    24661 ttgatgaagt caataagtct atagaaaatg tagctgatgt ttactatata aaaaatttat
    24721 tattgatacc atcacttatt gaagatagac ttatgttgat aaatgctata tataaagata
    24781 aacatcaaga ggatttttta gaaaatcaga aattactaat aagtaatatg tataatctat
    24841 atataaatgt taataaaaat aaaataaaag atgaagatgt tgaaaaaata ttgagagata
    24901 ccaattatat ttcaaattac aatgtagata agtatgatga aggaagaagt gtaattttag
    24961 agagtattgt aaatactaaa agtctaggtg acaagataat atgtctaaac ttactgcaaa
    25021 ctctaaatgg tgtcaaagaa atgtatagaa taagtaatat aactaaagtt tcaataaaag
    25081 aagcagctta gatattaaat tatagaatag ccatatattt aaaataaagt atatggctat
    25141 ttgttttata aaatactaaa agaaaataat atataaaaaa caaaaaacat atatttatga
    25201 attttatatg aaaatagtta aaatattgat ttttttcatt ggttgaaaaa acaatttact
    25261 taggataaac tataatagta ttcaaatatt atgggggtta gatttatgag taaagatgta
    25321 aaggtaaagc gtaataagaa atatgataca tttagtataa ctatggaact tgagagacct
    25381 ttactttatg atgaggttga gaacaaagat gataaaaata ataatagaaa atttaacata
    25441 aagaataaga tatttaaggg aataggtata ctatttgtat tgattttgct tggctttttc
    25501 atatggataa ataaaacata tgaacctcaa aagctggcaa aagaagctct agtcagcaat
    25561 agtaaagttg aagtaactgt aaatgaaaac atttcattta caccaaaagg aagaaaagtt
    25621 tctaaaggtt taattttgta tccaggagct aaagtagaaa tagaatctta tgctccactt
    25681 gcaagaaaaa tagcagaaag tggatatgag gttgtaatcg taaagatgcc acttaatcta
    25741 gctatacttg gaactaataa ggcacagaaa gttatggatt cttacaacaa catagaacat
    25801 tgggtaatag gaggtcattc attaggagga gttgcagcat caaattttgc acgtgataat
    25861 aaattaattg aaggcgttgt atttttagct tcttatccaa tgggtgatga gttgaaagaa
    25921 ttaggaaaaa aagttatatc tatatggggg agcaaagatg gagtagtcaa ttttaaaagt
    25981 cttgtagaat caaaacaaaa acttcctgat gatacaactt atgttgagat tgaaggagga
    26041 aatcatgcac aatttggaga ttatggaaaa caaaagggag ataatgatgc tattataagt
    26101 caagaaaaac aacttaatat tactgccaat agtatcatta aatttttaaa gaatatatct
    26161 taaactaaaa tttaaaaaat ataattttat aagttattat ttaaagtaaa tattaataat
    26221 ataatagaat gaatatttta ttacaagggg gattttaata tggaatacga tatgccactt
    26281 tacagaccac caagtgaggc ttatagtctt ataatacaag tcacactagg atgttctcat
    26341 aacaaatgta cgttttgtag tatgtacaaa tctaaaaaat ttaaaataaa gccactagaa
    26401 gtaataaaaa atgaaattga tatatttaga agatattata agcatgtaga aagaatattc
    26461 ttagcagatg gagatgcatt gataatacca atggagaaat taagagatat tatcttatat
    26521 ataaaagaag tttttccaga gtgtgaaaga attactatgt acggctctcc taaatctata
    26581 gagaaaaaat ctgatgatga acttaaagaa ttaagaaaac ttggtgttaa aatgatttat
    26641 ctagggcttg aaagtggaag caatgaagta ttagaagata ttaagaaagg ttttagtagt
    26701 gaacaattaa taaaagttgg tagaaaagtt aaaaaaagtg ggattaaatt atcagccact
    26761 gtaatagcag gtcttggagg aactaaaaaa acacatcagc atgcagttga tacaggaaaa
    26821 atgttaggag caatttcacc agattatgtg ggtgtactta gtttaatggt agaacctaat
    26881 actgaacttt acgatttact taaggcagga gagtttacaa tactagagga taaggctgtg
    26941 ttacaagaaa ttaaagagat gataaagaat atagatgcta atgaaaaaat tgtatttaga
    27001 agtaatcatg catcaaatta tgctaattta aaaggtgtat tacctgatga taaacaacgt
    27061 ctaattgatg aaattgatta ctatctaagt aaccaaaagc taaaaagaga agaatataga
    27121 agactatagt ttttgatata tcaatctata ttgataggta aatactaaga ttatttagat
    27181 acaaaaaatc atgctacatt aatagttgct tactattgat gtagcatgat ttttttatta
    27241 aaaaacaa
//
LOCUS       DAMJWU010000043        25633 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMJWU010000043 DAMJWU010000000
VERSION     DAMJWU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.043"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(283..663)
                     /locus_tag="PC460_002028"
     CDS             complement(283..663)
                     /locus_tag="PC460_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2600914.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(864..2057)
                     /locus_tag="PC460_002029"
     CDS             complement(864..2057)
                     /locus_tag="PC460_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2600915.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGIYLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            complement(2120..3376)
                     /locus_tag="PC460_002030"
     CDS             complement(2120..3376)
                     /locus_tag="PC460_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2600916.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            complement(4172..4906)
                     /locus_tag="PC460_002031"
     CDS             complement(4172..4906)
                     /locus_tag="PC460_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2600917.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(5912..6550)
                     /locus_tag="PC460_002032"
     CDS             complement(5912..6550)
                     /locus_tag="PC460_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2600918.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(6553..7410)
                     /locus_tag="PC460_002033"
     CDS             complement(6553..7410)
                     /locus_tag="PC460_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600919.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(7412..7786)
                     /locus_tag="PC460_002034"
     CDS             complement(7412..7786)
                     /locus_tag="PC460_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2600920.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            complement(8935..10125)
                     /locus_tag="PC460_002035"
     CDS             complement(8935..10125)
                     /locus_tag="PC460_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2600921.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(10141..12096)
                     /locus_tag="PC460_002036"
     CDS             complement(10141..12096)
                     /locus_tag="PC460_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2600922.1"
                     /translation="MEKIKIENSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(12113..14179)
                     /locus_tag="PC460_002037"
     CDS             complement(12113..14179)
                     /locus_tag="PC460_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2600923.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            complement(14506..15156)
                     /locus_tag="PC460_002038"
     CDS             complement(14506..15156)
                     /locus_tag="PC460_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2600924.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(16260..16595)
                     /locus_tag="PC460_002039"
                     /pseudo
     CDS             complement(16260..16595)
                     /locus_tag="PC460_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(16693..17373)
                     /locus_tag="PC460_002040"
     CDS             complement(16693..17373)
                     /locus_tag="PC460_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600925.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(17370..18296)
                     /locus_tag="PC460_002041"
     CDS             complement(17370..18296)
                     /locus_tag="PC460_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600926.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(18369..19286)
                     /locus_tag="PC460_002042"
     CDS             complement(18369..19286)
                     /locus_tag="PC460_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2600927.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRAIVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(19286..19975)
                     /locus_tag="PC460_002043"
     CDS             complement(19286..19975)
                     /locus_tag="PC460_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600928.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(20383..20678)
                     /locus_tag="PC460_002044"
                     /pseudo
     CDS             complement(20383..20678)
                     /locus_tag="PC460_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21752..21970)
                     /locus_tag="PC460_002045"
     CDS             complement(21752..21970)
                     /locus_tag="PC460_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600929.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(21971..22378)
                     /locus_tag="PC460_002046"
     CDS             complement(21971..22378)
                     /locus_tag="PC460_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600930.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(22464..22952)
                     /locus_tag="PC460_002047"
     CDS             complement(22464..22952)
                     /locus_tag="PC460_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF2600931.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(23157..24278)
                     /locus_tag="PC460_002048"
     CDS             complement(23157..24278)
                     /locus_tag="PC460_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2600932.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTQKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(24651..24821)
                     /locus_tag="PC460_002049"
     CDS             complement(24651..24821)
                     /locus_tag="PC460_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600933.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(24993..25436)
                     /locus_tag="PC460_002050"
     CDS             complement(24993..25436)
                     /locus_tag="PC460_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2600934.1"
                     /translation="MIEIKTIEINKKSQIDLLILADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         8848 a         4343 c         2693 g         9749 t
ORIGIN      
        1 tgttattttt aatcaaatga acgaatatta tatatatcat tcataaaaac tttatataaa
       61 gaatagaaac aagttctaga ttactataga gatgacatat caaaatattt ttaaaaagca
      121 atttaagtat tagaagtaat tgaagttagt aaatataaat gcgtttactt ttaagtattg
      181 cttaagggaa taaaaaataa aaataaactc tgcaaccatc taaaaatagt tgcagagctt
      241 acaaaaccaa aattatctaa attgtaataa aaataataac atttatacat aagcttcttc
      301 ccaatttact acccagtttt ctaacgactt tatcttttcc tctgttagct cctcttcgtg
      361 aaatgatact aataaattcc ttataaaatt attcttcaaa agattttgca catttcttga
      421 tataaactct aaatatgcct tttctttgat tgcaactgta taatacatac acttgcctgt
      481 ttcttgaaca tataagaatc ttttctttga tagttttgat aaagttttta aagctacttt
      541 ttctggtaaa ttatgttctt ctttcatata atcaataatc tcatatgaat tggtttttat
      601 atctaaattc caaataaatt tcataataac taactctaat tttgataatt tttttaaaca
      661 catagttatc actccgcttc tttatctata taattaaata ttacaataat aatttcacaa
      721 aatcaattct aataatcata acatacctta aaatctgttc ttaacttcta ccttcttaat
      781 ctcccaaatg atttttttat attgtattca atagtataat ccttttccaa aatataactt
      841 accagttaaa ataatcttta tttttactta ttctcgcctt ttacagaagt taataatttt
      901 ttataagtta catcaattcc aaaagctcca agaggtgcag ttataagtat agctagaact
      961 gctaacgtca aaataagttg tccagatgcc atccccattg caagtggtac acctcctatt
     1021 gccgcctgta ctgtagcctt tggaatatag ccaatcatac agaacaatct ttcctttatt
     1081 gtcaaatttg tcttaataag gcagaagaat actcctacca tacggaataa aagaactcca
     1141 aatattaaaa ttacagctcc aattcctgct ttaaatgcat aacttatatc tactgttgcc
     1201 cctaccaaaa caaacaagag aatttcagca ccaacccata gtttcgaata ctttatagac
     1261 aatcttcttg caactacacg atattttatc tgaaggaaaa tcccaatact cataactgct
     1321 aataatccag aaaatggcac aataccctct aaagcagtct ctaaacttat cattaaaaat
     1381 gagatactta aaagcaacac ccctttagca gaatctctca tatgaacctt cttaaaaaat
     1441 actgctaaat agattccaat cactgctcca actattacac ctaatataat tgatactgga
     1501 atttgtaaaa agctaatagc tgatatacta tttccttgtg ccaatcctgt gaatactgta
     1561 aacatcacaa ttacgaaaat atcgtctata gaagttcctg ctaaaagcat ctgtggaata
     1621 cttttttctg taccataacc ttcttccatt aatttaagca ttttaggtac aataattgcg
     1681 ggagaaactg ctcctactac tgcacccatg actgctgctt ccaatataga aacgcctagt
     1741 aactttggtg ctaaaactat cattccgata atttcaaaag tagctggaat aaagcacata
     1801 agtacagcgg gtcttcccac tttcttcaaa tcgtttatat ctaaagataa tccagccctt
     1861 gttaatataa taatcaatgc aatctttcta aggtcagaag aaatatctag tattgaatta
     1921 tcaatcaaat ttaaaacata tggacctaat ataattcccg taagtaacat tcccaataag
     1981 ttaggtaatt ttatcctctt aaaaatacct cccgaggcca ttcctaataa aaaaatcaaa
     2041 gctaaacttg tcaacatatt cctaccctat cctttctcta atattctttc tcttctaatc
     2101 atacattatg ttgttttatt tattgttttg ttgaaatttt ttgtagtgaa taaatagatt
     2161 tatccatatt aaatatatat gaaatagcgc atactacaaa gaaataaggt aaatagctgt
     2221 acccaaaaac ttctgcccct ataaatacag gtgcaaaaaa agtatttgta gcactaccaa
     2281 agacactagc atagccaagc gctgcaacta attcaattgg caaattaaaa aatcctgcta
     2341 acaatacacc aagactagct ccaatggaaa acaaaggtgt aacctctcct ccttgaaaac
     2401 ctgctgacaa agtcagtatt gtaagtataa actttaacag ccagtcaaat gaataaattt
     2461 ctccaccata gaaactattt tgaattaaat ttgtccctaa acctgaatat cttcctttat
     2521 aaaataaaag gaataataca ctaagacata ctccaataat agcaatcctt atcataggat
     2581 tttttaatct attccctatt tttctttttg acaatttcag acaccatgca aatgctccac
     2641 caaccattcc aaaaataatt cctaaaacaa taagcttcca aaatattgac aagtcaaata
     2701 caactttaga agaaagtgca aaagaaaatt tctctaaccc taaagctttt gatactgcac
     2761 ttgctgtaaa agaagcagta aacgcaggaa aaagcgattg atactccagt gaaccagcaa
     2821 ccaaaacctc catagcaaat agtattgctg ctattggtgt ctcaaataat cctgcaaaac
     2881 cagctgccat accagttact agaaaaatac tagaagcatt ttttataggc aaccttttac
     2941 ctacccaatg tgaaaaagtt gcaccaatct gaacagcaac tccttctcgt cctgcactac
     3001 caccaaataa atgggttaac catgtacctg atattataaa tggaaccaat cttaaaggaa
     3061 taatttcttc ttctccatgc cctacctcaa atattagatt cattccttta ctactttttc
     3121 ctccaaattt aaaatagcaa taagcaataa caactcctac tactgctaaa aatggtatca
     3181 gatacattgg atatgtttct cgaatatctg taacttttag aagaaccgtt ccaaatatgg
     3241 tatctataag acctataatt gcaccaactg gtatacctat gagtcctaaa aaaaatagtc
     3301 caccatatgt tttataaacg cttttaattc ttattatcat tttaatccat attaaagttt
     3361 taaatttttc tatcatttta attcctttct atttgcatca taagattttt tgaaagtttt
     3421 attacatcaa ctgtagtcaa tgtaatgact atcataattg ttttttaaat tgataggata
     3481 taggtacact cttaacccat tcttaataaa ataattaaca tataatctac tattttttct
     3541 attaattaaa tatttcttca aatttaaaaa ataaaaattt agtaagtact tcacctccat
     3601 tttatcgatt taaacaacaa aaaaagctgg tggcatctat gatatcatag aagtcatcag
     3661 ctcattggca gtttaggata ttatcatcca agggagaacc tcattcccat aattattata
     3721 taatttttta tttttcatat gtctatttta tgtaaattgt atttttaatg ttataaataa
     3781 tataacctat caattaaaat attaaagtct ctaatattat gagtgttcaa tacttcatat
     3841 tatttttatg atatataatt taaatgattt tctttacttt gcattatgat acatgattta
     3901 aatatttttc ttcactttgt atactaaagt ttatttaaaa tattttcttt cctattatac
     3961 tatgctgtta aatatttttc aactgaagta ttgtagaact ttaaaattac tataaaaaat
     4021 tatattctaa aatacttgta atactacata tcacccttaa cacttaagta taattttaaa
     4081 gcaaaattct tattacttat atttaatttt gattctacat ctttagatat aattttaagt
     4141 aatttaagaa attattgtaa atcatatagt actactcaat aattaggttt ttcaatgttc
     4201 caattatgtt tctttgatat aatccaccat gactaaaaac tatcgtttct atatcataat
     4261 tacttatatt tttaatagca tcttttaact cttgtttgtt gctgtacatc acatcaacag
     4321 gcttaagttc tccattttct acctgtaaca aatctccagc aataagtact ttagactctt
     4381 ttaaataaag acatatgtga cctctagtgt gacctcctgt atgtataact tctatttcct
     4441 cacctagatt caatttttca tggttatcta atgacacatc tactttagta tatgatgatt
     4501 taaatccaag tcccataaca tttagcatac taagcatctt atcatcaatt ttatctacca
     4561 tttgttccaa catataaagt ttaaaaggag tttcttctcc tgttatatag ctgatttcac
     4621 ttttgtatgc atatactttg atattattct tagatttttc tcttagataa tttatgtttc
     4681 ctatatggtc tatatcatga tgagtgatta ttatattttt taacttatca aagctcgtgt
     4741 tacttttatt aagagcatca attattaaat caccttgacc cggaagacct gtgtcaataa
     4801 gtgttgcatc atcttctgtg tagattacag gaatatacat aactttatct tcaccgaata
     4861 catttgatga aatttttaaa acatctaatc cctttgctaa attcataatt tttctcctta
     4921 taattactta attagaggtc aatttaatta aagaaattga gaaaatgtag attttcctct
     4981 agttatattt tattaattaa aaattattaa tacctagtat atcatttaag ttgaaggatt
     5041 actcgacttg atttttttgt tattttatgg tatagtaatt aatatagaag taagtaattg
     5101 caagctttgc aattactttt atttttttct aataactatt ttatttttga taattcgtat
     5161 aaaattaaaa gtaaagtaat atagaaaata tgaatataga aaaactttaa taagaaaata
     5221 aattatgaat aaataaaaga aacgcaatac tgtatataga tgattattta gaaaaataaa
     5281 atctctttta ataaaaataa gcttcaaaat aaacttttag ttcataaagc ttaggagtaa
     5341 ctaaattgag tgcaagtgta tctactgttc aaaataaata gttcataaag cactcttttt
     5401 tatattaaat caattatatt ttcgtcattt cagcaatgaa aaagaagctt atctctattt
     5461 tgagacagct tctttttatt aacgtcctac tttttattag ttttctaaaa ttaaaaagct
     5521 ttatttatag gattccttag atttatattt tgacgatata attttttaaa aacaccttaa
     5581 aagatggtat acaatacaca caatcatact cataattgaa aaataatcaa tggaaaacaa
     5641 aattactgga tattccctat taaaaaatac aaattgattc acaagaaaca tataatctat
     5701 aatattatga tgttactctc ccacaccaag taactttatt ttcaagtaac cttttcatac
     5761 catctaactt cactcaataa aaatggcaat ataaatatat actctatttt ttacttttaa
     5821 atatctgaca agctttatat actattggaa gtaagataca aagtaaaaca gcccaatctg
     5881 gtaaatgtgg catataaaag tcccccttac cttatacaag ctccttatta ctaaaaattt
     5941 taatagaaat aaacattgaa gctaacagta taattaaact taaaaggaac aatacaatat
     6001 tcactatcat ggatggatac tgatatataa atggaatttc aaaattgctg tgagctatag
     6061 ttaatggtaa tacaaatgat gaagccatga ttatcatcac gaaaaagaat tttgttgttt
     6121 catatcctaa tttgtattga ataggcatat aaactccata aataatagaa cttattaata
     6181 aaacaatcaa aatatatgtc aagtcaaaag ttcctatttg tggaataagt aaattttcaa
     6241 tccagtaaac taatgtacaa tagataaata ccattgcaaa taaaacatac ttagattgaa
     6301 ctaagccacg tctaggataa ggagttgaac aaagtaatac agatgctttt gaatattgac
     6361 tctcctttaa taatagatac tgacacagca taaattctga aaatataatt gatagtacaa
     6421 atcccaatat ttgtgcaaat ggaggtaaac gccataatat aaataatggg attcctattg
     6481 caataaataa tatcaacaat acatattttt ttacaatcag aatatctttt ttaactaaat
     6541 gaatcaacat aattactctc tcctttcaat atatgcaagc ataatatctt caattgtagg
     6601 acgctctatt aaaatatctg gcatattttt ttgcacttca ttaatttttg aggtaatccc
     6661 tataaaacca taacctgtaa tttctaaatt taaaaataat tttttggttt catcatttag
     6721 aaattgaata tcccctttta cttttctatg ttcgtccaat agagtatctt tttcacgctc
     6781 taatataata cgtccattgt caatcataac aatcatatct gctattttgt ctaaatcaga
     6841 tgttatatga gtggaaaaga aaacaccacg accacatttc ttcatatatc ctgttagtat
     6901 gtcaagtatc tgactacgga ttagtgggtc taaaccactt gtaggctcat ccataattaa
     6961 aaactcagct ttatgcgata atgctaatgc taaagcaaat ttcattttca ttccttttga
     7021 aagtgtatca atcttttgtt ttgggttaag attaaaacgc ttcatatttt gttgataatc
     7081 ttcttcacac caattatcat atgaaggtgc aataatactt ttcatttctg ccattgacaa
     7141 ctcctcatag aaatatgaat catcaaagac aactccaatt cgatttttta attccttctc
     7201 atttccatcg aaatctttac caaagatttt tatttttcca ctatcacgct ttacgacatc
     7261 caatatcgaa cggattgttg ttgtttttcc tgctccatta acacctataa atccggttat
     7321 acaatcttct ggtaatgcaa agcttatatt agataatgaa aaatcggtat aagtcttatt
     7381 taagttataa acttctaaaa tattttccat attaatcctc ctcatataga atatccatca
     7441 tttcttgtaa ctctatctgt gtaattccta gtgcttttgc atctttaatc atatctgcca
     7501 ttttttgttc aactatacgt ctctttgagt ctcttagtat ttctaaattt ccagttgaaa
     7561 taaatgttcc tattccaact tggcgtgtaa gaaagccttc attttcaagt tcttcataca
     7621 ctcgtctaat agtcaaaata ctaactttta aatcattagc aaaatttctt atggatggca
     7681 ttgcatcccc ttctttcagt tcttcagtaa atattgcttc tttaatttgc tctttaattt
     7741 gttggtataa tggcttatct gatgtgttag aaatgagcac tttcattttt tatcaccccc
     7801 tttttatcac tttcagtgtg atgttatcat atacacacta tacacttaga gcactttttt
     7861 gtcaatataa aagatgtaaa atattactat gacaaaataa atatgaatga aatattagag
     7921 attgttaata gccataaaca ctttttaaaa ctacataaga taggatgtat taaatattta
     7981 ttaaaagtaa gaattgaata tataaaagaa aaagtaatcc tgtatgaatg atttgtttaa
     8041 tgataaatta agttataaac tatttataaa aaacataaag gaataaatcc atttctaatt
     8101 acaaacatac taaaaaattt agataataat aaataaatag taataaatgg gatgaattta
     8161 taagaatttg gatagaggaa agagttattc aagagtgtag actagaggta aactctatat
     8221 taaaaaaatt taaattggga tattattatg tatggaatat ttgcagaagt aaccttgcta
     8281 taagtataga agattttttt gattacaaaa gataatactc taaaatgtga ggatttcaac
     8341 attcaatact gtgctttaaa taatatcact ccaaaatacc atccaccttt tactataatt
     8401 gaatacccta attattttaa aaaagaaaac attagaaacg gaaaaccttg tatatataaa
     8461 ataaaaaata ctaacttcta aaacacgaat actctttgat aaataaaaca aaaaataatt
     8521 ttaataatta aaattggcta tctcaaacta gagataagtc tctttctcac ttttaaaacg
     8581 ttgaaaatat attcgatttc gtacaaaaaa gagtgcctta gaactaaaaa gttcactttg
     8641 tggcactccc ttttttctcc tctttttaat tttttataaa aattcttcaa aaatattatt
     8701 aaatacccta tattatacat aaatcaaatt aaagttgcat tttagcaaat aaaaacaaat
     8761 tataactatt agcatagcaa ataaaaaatc tgctatctta taaaatcaaa attataatta
     8821 taaaaagaaa atattcttat aacaaattaa ctaatataaa attattttat aaattaatac
     8881 tactttttag cttctcctat aaattaatac cactttttta actatgaaac ctatctacaa
     8941 cattggtacc ataagaccag ctaccacaat agaaatcata caagccatac caccaactac
     9001 cagcttaggc ataatcttag acatcaaata tgctctttct tcttccgttt ctccaacagc
     9061 attacacact tcatttgaaa ttaaatatgt agttgggaat cccaacatct gacaaaaccc
     9121 tactccaaaa gcaagaggtt tagaaccgat aattttccaa cctggtaaca ccttcatcat
     9181 aaggaatata ccaatgatag aagctgcaaa catacccaca attggtacaa aaagactaat
     9241 taaatctgtc aaagaaactt ttgctaaaga aggaataaca gctgcaaaca caaccatgtt
     9301 gataaatccc ttgatttttc ctttttccaa aatgtctttt ggcacaatac caatacgtgt
     9361 tacaataaca ccaacaacaa gacatacaat agaataattt acatgaataa tctctgaaag
     9421 ttcataccct agataagaga ataatgctat aaaaaatata caaacatttg tagaataata
     9481 tttatcgtat ttttcacaga aagcttgttt tacttttgtg cctgtattct ctgcttcttt
     9541 cttatcagta tcaacatttg ctaaatcaat gccttttgat tttgcttcat gatattctac
     9601 taaaagacgg tttgcctctt gtaacgctgc tcttgaagct attggtgtac caataaactt
     9661 ctgaattgca aatacaactg ttgctgtagc tgctgcaagt gtcagtcctt tttctagtgc
     9721 tgcttgtgtc ataatagtag tagcaggtaa agctccattt ataactggta tgcttgtcaa
     9781 tactgttgat tttccaaaca aaggaataca taatgagaca cagactataa ctgctacaat
     9841 tccaagccat gctgtcaata ctgttctcca ttcgtctata aattgtttaa cattcaacat
     9901 tgttcccata ctaaacataa tcattggaac actccaacta gctgctgcgg tcatacctgc
     9961 tttttcaata atatcagctg gtattgcttt tgtgacaaat agaatcaaaa ataaaagtag
    10021 tgtaacaaat acagctgata cttttgcttt tgttttgctt gcaattaaat ccccaatagc
    10081 aaataaaact aatacgacac taaaccataa taacatatca ctcatagaat aaccccctta
    10141 ttataaatat ctttcaaacc aatctacaat agcatttgct cttgcaattc tattagatgg
    10201 tcttccattt ctagacaatc catgtgtatc tccatgaaac aaatacattt ctgtatctac
    10261 accaagctgt tttaatgctg taaacatttg gaaagtatcc cccataaaac atctataatc
    10321 cctatcacta tgtaagaaca atgttggtgt cttaacattt ttcacatatt taataggtga
    10381 atgattccaa agaacctcct catgattcca aggcgtctta tctccacaag tctgcttcat
    10441 aattctatcc ccaaagtcaa taattaaaga agctgtaatc aagttactaa tacctctttg
    10501 agaaactgct gctttgaaac ggtctgtatg accaattatc cagtttgtca tataaccacc
    10561 gtaacttccc cctgcaaccc ctaagcggtc tgcatcaatc ccaggatatt tttcaataca
    10621 agtatctaca aattccataa gatgctcata gtcaacatac ccccatcttt ctaccatttc
    10681 taagaatttt tcaccataac cgtcaccacc atgagggtta caataaatca catacattcc
    10741 ctttgatgtt acacactgct tcaagtgtgt aaacatatag ccatatgccc atttaggccc
    10801 accatgaatg aacaatacag caggatattt ttcgccttct ttagcatcta taggtggtat
    10861 aacaaaacca tcaacttctt ctttattttt tgctacaaac gtcaattttt caggtaatga
    10921 aaccttatga gtatctaaat atggtttaga aaaagcagtc atttttttca ttgtacctgt
    10981 agtcatatca atgctatata tttctgctaa ctcataatct ttcaacatta ctgtgtacat
    11041 ggtgttgtcg taaatatcaa aagatgtaaa agcatctact tttgtgatga tacgtatatt
    11101 acctgtcttt gtatcaaact cattaaattc tgtcatagtt ctgcaaattc ttgcaccaat
    11161 aaatttgtct cctataactt tattactacg tccaccacca tatactgcat ctacatccag
    11221 caatgtagca tacataccat ctggactagc aatcatattt atttcattag tactcaaatc
    11281 aagtataaaa atatcatgat tctgtgtgat agaacggtct aatgttgaag cttgcaacat
    11341 tactttatct ttgccaatat attttgccaa acttatttgg tactttcctt gtggtacaag
    11401 ttctttggtt tcaccactgt ctatatgata ctcccaaagt ccagaaaaag gagttgcaca
    11461 atcttcataa atctgaccag tatataaaat tctgttttta tcagaagaaa cgtctacacc
    11521 tgctacttca caaaattttg gtgtaatggc ttttgttata ttttcacctt cttgatataa
    11581 ttctaatgtt acacgtcttc tttgagaaaa attttctcca tctcttataa aaggtttatc
    11641 tgtaaatgtc caataatcta caccctctac tgccctatct ggttctgatt ttttagaatc
    11701 cgttggattt tttgttgtca gtaaccacaa tttatttcca agtgatacag gtacgctaca
    11761 agcttttgga atagaaaatg ctttttttgc ttctcctcct tctatagaaa tagtgtaaaa
    11821 atctgttgta cctgattttg gcttatttct tttagatgta aaaaggagtt ctgtattaga
    11881 aatccaacta aatgatgtta catcttttgt ttgagtcata tgtatttgtt tttctgtttt
    11941 gatgtccatc acatataagt catgttcata gcagtctttt tcctcgttga tatttgatat
    12001 aatataagca atatgctttc catcaggtga acaagaaata tcatgaggga atatatattc
    12061 aaaaatatct gaattttcaa tctttatttt ttccattttt atattctcct ttctactgtc
    12121 ctatgttatc tttttttata tatactgaaa accaatctag catttcaact aatctttgta
    12181 gcttttgttt tggtctacca gaacgagaca gttcatggtt ttctccttca aatagacaaa
    12241 gttttgtagg acacccatga attttcaatg ctgaaaacat ctggaatgct tcttgcatat
    12301 agcatctata gtctttttca ccatgtaaaa ataaagtcgg tgtaacacat ttatcagcat
    12361 aagcaagagg tgactgtttc cattgaagct cgtggttttc catatgagaa gcacctgttt
    12421 gtcctttgca gaaataataa ccaatgtctg ttgtaccctc catgccactc cagtttgcaa
    12481 tagaacgctg agcacaagca acagcaaaac ggtttgtatg accaattatc caattcgtca
    12541 taaatccacc ataacttcca ccttcaacag ccaattttgt tttatcaatg tctgtatatt
    12601 tttcaagtac agtatctgta aattccataa aatctatata atcaatatct ccaaactgtt
    12661 tcctaatatc tgcaaaagca ttgccacgac cttcagaccc ccttgggtta cagaagaata
    12721 caaagtaacc tgcacttgta aatacctgta tctcatgcat aaaatatggt ccatatgtca
    12781 atcttggtcc tccatgaatc gccaatacag ctggatattt tttccccacc tcataatcta
    12841 ctggtttata tacgtatcct tgtatttccc atttagaact tccttttgac tctatgtatt
    12901 ctggcacaga aattttatat tcctctaaaa tatggtcatt taaatgtgtt ttcttacaca
    12961 atgttccatt ctctactgta taaacttctg gtaatccatt tcctaaacaa cccacacata
    13021 caatattgcc atcttttaca tctataaaat caactgaacc tgtctgtgtt actttttcct
    13081 gttttcctgt tggaatatgt attctttcaa taatgcagtc catatctact gtagaagtgt
    13141 aataaatata ttctccatct acctttgttg tctgaccagc acccatttta acatcagatg
    13201 ttactgtatt tccaatacaa tgatgttgat gccctgaaag aagtttcatt tctttcgtat
    13261 caatatcaat agtataaaaa tctccatttt ctccatttcc ataagataaa gcatcagtta
    13321 ggtttagtat cacttgattt tgatataatt caaaaccttt tataatatac ttatcttttt
    13381 ctaatataca gacatttgtg tttgtgtttg tatcaaatac atagacccca tttttcaaac
    13441 cttttacatc tttaaactct tgccctatat ataagatttt tccatcactt attttcatac
    13501 ctacaacatt aaacataggt gctgtaattg ccttcaactc atttatatta ctgtcgtata
    13561 catataggcg ttttcttttt ctgctaatat atccctcccc attgacagca taaggaattt
    13621 ctttgaaatg ttcataatgt cttttgttat aagcatttat ttttttctgt tgttcttctt
    13681 ctggcaatcc ctctacaata ggtcggtcat tgtcataaga atcaataact gcatacaaac
    13741 cttttcctat aggattcaat ttaccacctt tgatagggag tctaaaaagt tcttctgctt
    13801 ctccaccatt taaagataaa gcatagtaac tcatgcattg cttaccgttt ttgacagctt
    13861 cttttacttt ttcatttcgc aaagcaggga acaagatagt attttcatct tcccaggcat
    13921 atgctccaac agagtttata gacgttattt gttttaattc gccagtttta ttgtttaata
    13981 tataaacttg gcttgtgtaa ccattctctt tgatatcagc tttttttaca ataaatgctg
    14041 tatatcctcc atctggtgat atttggcaat cacttataaa ttgatattcc aaaaatgttt
    14101 cactcgtaaa tagctttttc atagtatttt ctatcctttc tttagtaatt tttttcaaac
    14161 tctctaccac ctctttcatt ttttatttta aatttaaaat aaaaaatttt aaatttaaaa
    14221 taaaaaattt tataaaggtt atttttttag gaagccttct ggcttccttg taatatttgt
    14281 aatttaaagt atatttaatc catgcttaat tcatgcttaa ttcatactta atctatactt
    14341 aatctatact taatctatat ttaattcata cttagactta tttacactat atttagatta
    14401 tatttattag attatattta gattatgttt aaactataat tagattatac ttagactaca
    14461 tttagcaact taatagataa gtatatatag taacttcaaa acttattatt catcttcaca
    14521 gaatctttta atagttcctt gaatatgatt tcttagttta tctactgctt tttttgtatc
    14581 tctgtcttca atgaatttta atatttcaaa atggtcttgt actgttcctt cataattacg
    14641 attttttaat ggtcgaatta aatagttcac tactataaat cttccttgta taccaacata
    14701 tacatcattt aaaatctcat tatttgcccc ttctacaatg gctttatgta attccaaatc
    14761 tgctagtgca agttctacag atttttcttg acgatagtac tcctcgcatt tatcagagta
    14821 tctttttatt acatctagct tttgttgaaa aacctcttcc tcttcttgca tcattttttc
    14881 taaagcatat gtttcaatca gtgttctcgt ttcacaaact tgttttacca tatcatattc
    14941 atactctggt acgtacactt taccatttgt cataatagta agcactccat ctttatttag
    15001 catttttact gcttgtacaa ctggtgttct gcttacttca aatttttcac aaaggtcatt
    15061 tagtaatatt ttttgaccta cagagtattc accacgaata agactttcaa taatcttatg
    15121 atataaaatt tcatctaaat ttgactttac tttcactact ctcaccttat ttctattctt
    15181 ttgccatatt atattgaatt actggacttt aattactttt agtaatattt ttaaagtaga
    15241 aattcttatt tgatagtact tttgtactac aatttaaatt attgtactgt aaatcatgct
    15301 cttatactat agattaattc attgtattgc aaatcaagtt attgtactat agattaaatc
    15361 gttgtacttc aaattaaact attatactat agattaaacc attgtacttc aaattaaact
    15421 attatactat agattaaatc gttgtacttc aaattaaact attatactat agattaattc
    15481 attgtacttc aaattaaact attataccat agattaattc attgtattgc aaatcaagtt
    15541 attgtactat agattaaatc gttatacttc aaatcaagtt attgtactat agattaaatc
    15601 gttgtacttc aaattaaact attatactat agattaaatc gttgtacttc aaatcatgct
    15661 agtgtactat agattaaatc gttgtacttc aaatcatgct agtgtactat agattaaatc
    15721 gttgtacttt aaattaaact attatactat agattaattc attgttctgc aaatcatgct
    15781 agtgtactac aaattaaact ataaatgttt actatctctt aataatttga ataatatgtt
    15841 acaaaaagta tagcaccttt ttattttttt cgcaataaat aaaaaattta aaatttttta
    15901 ttttttattt tcttatatta tcaatttgaa atatgaaaga ttaagactaa tagaattgat
    15961 tcagttaatt gaaaaaaata ggtacctttt atgaaaattc taatgataag catttttctg
    16021 atgtaatggt aaattataaa tgataaaatt aaaaatcact tttagtttta tgaactgtcc
    16081 ccatattaag aaacaactaa gatacataaa aatagttata aagagatagt taaactatat
    16141 ccactatttt atatagttaa aatagtgggt atagtccaag ctaagaataa ctaagaaatt
    16201 agcgttaaat agcatagata aaaatatatt tatataaaaa ctttttttgc tgcaaacaat
    16261 tatttccata acattttata tacttctcta aaatcttcta aacttgataa atattcaatt
    16321 aaagggacat aattacctaa actacaagaa tttaaggagt ttctatacaa ttcctcacta
    16381 tcccaagata caatttttat aggaagatta ttttctaaca tctgttttag cataacaaat
    16441 ctacctatgc gaccattacc atcttgaaat ggatgtatca attcaaatct ataatgaaat
    16501 tcagctataa ctttaataga tacttcactc tgactatagt accattcaat taattcatca
    16561 agctttggct caacttcaaa tggttgtgca atattattaa aactctaata atattaataa
    16621 tagctgatat aatagcaaat cattattatc ccaagaagat acaattaata actaccctcc
    16681 tatccacagt atctagtcaa tttcagtaac tttatgattt agtatataat atataccaat
    16741 cacagttata ccaactataa taaaaacaga aatcaaactg tcttgagttg tatttgtacc
    16801 agctaaaata aattgtctta aattgataaa aataattgaa ctgacaaggg taacaggtat
    16861 tgattttctt atcattccaa caacgaatgt ccatatacct aatgagcaaa atacaataat
    16921 acttttaatt gctcctggta tccaataaga taaaattgaa atatgaaaat ctccaataac
    16981 taaattaaaa tacttatcca acacaacaac gaatgtaaaa caacagatat atccaactat
    17041 cattgatgat atcataagag tcgtaatcaa aagtaacttt gataaaataa gttttttacg
    17101 gtcaagtgga taagagaata aaatcaagat tgttttattt gtatattcat ttattatata
    17161 tgccacattc aacacagcaa ataatataat atagtaaaaa cagaaaaata aactgattgt
    17221 cctaaatgta acatcataat catatattga agtagattta tcattcaatc cagtaaagac
    17281 aaaaaacata cataagaaaa tccccagcat tgaaaataaa aaatatcttt ttagcccaac
    17341 tttttttagc tctagtttca ttaaatgtat catagtgttt ctttacctcc tgtacctgta
    17401 gtttggaaga agtaatcttc taaagaactt tgtttacgtc caatgctatt tataccaatt
    17461 tgattttgaa taaatatttt ggatatttct tttacatctt ttcgtaaatc atatatttca
    17521 atacaacttt ccgaagtaat tttaaagtct tttattccaa attctttttc taacaagtat
    17581 ccagcaagct ccacattatc tacatctact tcaacataat ctgtttgata tttgtgtata
    17641 tctgacatag aaatttcttt aataagttta ccatcttgaa taattccaat agtgtccgca
    17701 atttgttcta cctctgaaag aatatgacta gaaataaata tagttattcc ataatctgta
    17761 tttaacttac ggaataattc tcgcatttct cttatcccct ctgggtcgag tgcatttata
    17821 ggttcatcaa gaatcaataa ttctggttta gtaagaattg ctctagctat tgcaagacgt
    17881 tgtttcatcc ctaaagaata ttttgaaact ttcttgtttg attgctttga caatccgaca
    17941 cattccaata cctctgatat atgttctttg ttataataac ccatatattc acagtgtaaa
    18001 tcaagattct gtttagcagt caaattctca tataaatacg gattttcaat aatagtacca
    18061 atctttttta atatttcaaa atcagtttct gccactttct taccgaacat aactatttct
    18121 ccagaatttg gattaacaag atttagcaac attttcatta ctgtactttt acctgccccg
    18181 tttggtccaa caaatccata aatttctccc tttttcacat gaatattaac agcattaaca
    18241 acagctttat ctttatatat tttctttaat tgatgggtct ctatgatata gttcatcata
    18301 ctccctcctt tctaaaaaca gtatacaaaa taaaattgtc ttttctctct tttaaatcta
    18361 acttttttct aacttttcaa ttttaccgta aaaactgttt ttacattagg tctactattt
    18421 acataaattg aacctcccat agagtttgca agcccttgag aaatagccaa acctaaacca
    18481 ttgccttttt ttctgtcagc agtataagct cttgtgaaaa tatgctcttt ttcagtttca
    18541 gaaatacctg aacctttatc ctcaatatct ataaaaacat gtttttcctt ttcatatagt
    18601 gaaatcccta aaaatctacc atctgaacca tattttatag cattatcaat taaatttttg
    18661 agtatacgtt gtaatgcttc tgtgtcaaca tttgcatata taggtctatc ttcaatatta
    18721 acttcaacat taaagttttc tttatctagc aaatcataaa actctaaaat aatctcatga
    18781 cataattgtg ttagattgga tttttgaaga tttaatatca tatctccaga ttggattttt
    18841 gccatattaa acaggttatt tattgaatta acaagctcat ttgaatttgt ttgcattttt
    18901 tccacaattg ctctcattga agaagataat tcttcttttt gactttgaag atatagcatt
    18961 tcaatataac cctttaatac tgtgatggga gttcgtaaat cgtgtgatat gtttgtaaat
    19021 atttgcaata tcgctttttc acttctatta taattaattt gcctgctgta aaacttttca
    19081 acaagtttat ttatatctac tgctgtttct tttacaatcg aaatatcaga tggtactaaa
    19141 atataattaa tttcatcatc atttgttatg tcacgaagtc ttgaattgat atacttataa
    19201 tcactttcca ttttctttga tttatttcgt tcttttaagt agagagcaaa tagtacaatc
    19261 gaaattacaa ctaaaaataa ctccattatt cttcctccat tttgtagcca attccccaaa
    19321 gagttttaat ataattattt ttttctatac catcagacaa tttatttctt aacctgttaa
    19381 tatgagtatt tagcacattt tcatttccat aataaggttc tttccaaata gcgttgtata
    19441 attgttcctt tgaaaaagca cgtccttgat tagtcgctag taatttaaca atttcaaatt
    19501 ctgtatgtgt taagtttaag tcaattccat tctttgaaac tacatatttt gatatgttta
    19561 taatcaaatc cttataccga ataaccatat cttgagagtt tcttgaactg ctatacactg
    19621 ttgcacgtct aatattagct ttaattcttg caagcaattc tacgattgaa aaaggctttg
    19681 taatataatc atctgcacct agattaagcc ctagcatttt atctgtgtca ctatctttcg
    19741 cagtagtaat aataacagga acaacactca cacttcgtat atattttaat acttcaaaac
    19801 cactagactt tggtaacatc aagtccagta aaataatatc aaatgaataa tttttaaata
    19861 tttcaatagc tgtatttcca tcaatggcag ttttaacaga gtaattctca ctaatcagaa
    19921 aatcgtatac catttctcga atattcttat catcttcaac caataagatg tccattatta
    19981 ttcaactcct tttcgtcagt atacttcaaa tgatatattt attaaaaagg gttgtcaata
    20041 aataactgct tatgatattg tatctgcaat ttggtgatac tcttctaagt taaagttctt
    20101 ttcaataagc tttattgaaa aaagaattaa aatatagtat actctcttat ctttatctaa
    20161 aatatacata attttctttt attaacatct acaattaact attttatatt ataaaacacc
    20221 ttttaacttt tgctaaaaaa tattttatac ataaaaaaga aaccacattc tagttttata
    20281 cagaacatag tttctttaaa aataatcaaa tttggatttt aaataattaa aaacaaactt
    20341 ttatttgttt ttaattttcc aattttatag tagataattt agttaattag ttcttaaatg
    20401 tttatcttct ttagtaaatt tttttcttat taagaaatat gcaactatta caacaagttc
    20461 tattgctaaa aatacaccat aagtagctat agaatctatg tatctatgcc atttctaaag
    20521 aaaattaaag caattcctgc tgctataata cctacagcat tttaattttg accaaaacca
    20581 taaaaagaag ggaaatttcc ttttatttca atgaatgtca agattaatcc aatagctata
    20641 aatagtactc ctacaacttc cataatcatg ctaggcattt tgacccctcc ttattaataa
    20701 ttaactacta ctataaaaat atttattatt tttataatta tattatatat gcgattacat
    20761 gtttttggaa gtatatttgg aagtaatttc atatttattt aacttaattt ttttgtgata
    20821 aaataattct aattttcata tagatattat tataaatttt ctagtttatg gagttattca
    20881 ttctataatt cacaaatatg tattttttaa tctattaaac tattcatttt ctggcttata
    20941 agcatggact tcttatctat aagcttggtt tttcatctaa taagttattt attctttaat
    21001 ctatgagctt gatttttcat ctataagtca tttatttttt aatctataag tttagttctt
    21061 catctataaa tcatttattt tttaatctat aagtttggtt tttcatctat aaataagtca
    21121 tttatttttt aatctataag tttagttctt catctataaa tcattcattt tttaatctat
    21181 aagcttggtt tttacctata attaattcat tctctaatct ataagcttgg tttttattta
    21241 taagtcattt attctctaat ctataagctt ggtttttatt tataagtcat ttattctcta
    21301 atctataagc ttacttctta atctataagt catttattct ctaatctata agcttacttc
    21361 ttaatctata aatcattcat tttttaatct attagcttgt tttttaccta taattaattc
    21421 attctctaat ctataagctt tctttttcat ctataaatca ttcattcttt aatctatgag
    21481 cttggttttt catctataag tcatttattc tctaatctat aagctttctt tttcatctat
    21541 aaatcattca ttctttaatc cattagcttg gtttttcatc tataagtcat ttattctcta
    21601 atctataagc ttacttctta atctataaat cattcactct ttaatccatt agcttggttt
    21661 ttcatctata agtcatttat tctctaatct ataagcttgg ttttttacct ataattaatt
    21721 cattctctaa tgcataagta atacatgaaa tttaaatatt attttcttca ataaattcaa
    21781 acaactcttc tacagttgta tcaaatacct tagcaatatc cattgccagt tttaaagaag
    21841 gattgtagcg accattttct aaatgtacaa tagtttcacg tcttactcct actgactttg
    21901 caagttcacc ttgtgtcata aggttctttt ctctatagga tttaattttt gtctttagta
    21961 atactgacac ctaattgtat atcccctttc tgtcaaaata tataaataga attaacttca
    22021 acaaggattg aataaacaaa atgactaata aagacattcc tatatttaaa tttgacacat
    22081 ttatgtccaa agttacaaga aatatagata ctatagctac agaaaaaaac aatactttta
    22141 ttgcaatatc attaactctt gatagtatac attttgatgc ctcatcaatt aaatcatcat
    22201 actttttatt atagacccac tgagctccca tagctaatac ccaaataatt ataggaagat
    22261 attcaatact acttccttta aaaaaatact tatttactaa tgtagctcca atacaataag
    22321 aaattcccaa tatttctgaa ataagtgttg atttaatacc agtttgttgt ttagacatat
    22381 aaaatccccc ttaaaaagtt atgtatttat aacacctctt gttataaata tataactttt
    22441 ttgaataaat ttcaataaaa atattatttt gcaacaaatt cttttattaa ttttacatta
    22501 gatagaggca ctttagatgt cactccttgc aatgtcaaat cttctatatt aacttcattt
    22561 aattttgaac acagttctaa gtcaaatatt ctttcccaac ttaccttctt ttcaatttgt
    22621 tcatcttcat aaagctctac agtaatatcc attaaaggta aatgccataa atcaaattct
    22681 gataataaca cattgtcttc acttatttct atttcaagta aaacaccttt ttttcctttt
    22741 tctaaataac cactacatct taaatcaggt ctcttcgtcc aaacccatat catactatca
    22801 tcaaattcat atattctatt tgtcatttga ctatacatcc atttgtatgg aatatcaaag
    22861 tcttctaata taaaagaata atcatttgat agatatttat tgtttttatt gttattccaa
    22921 aattctaaag tctgtatagt ataaaatttc atatataaat caaccccttt gtttttctat
    22981 aagaaggaga tggttcaaac ttttattttg aattatctcc tttatttata cacctctttg
    23041 tacctattat ttatatatct ctttatatct accatattta aataacaaag ttttatatta
    23101 atttaaattt aaaagaggaa gctttgtatt tatctagtgc catctcaatc atagtattag
    23161 ttttgattat agactttttc aacctctgat ttattcaaat catctataat atcgcatatt
    23221 tcactcaaat tgtatccttt attttttaat tttatgatga tattaaattt ctcttctctt
    23281 ttcccttttg caataccttt ttcgatacct ttttcgatac ctttttcaat accttcttga
    23341 aaaatttctt ctttaaaatt tctactttta ttcataatat acgcacctct ctttaaattt
    23401 aataaatagt cctcattact acctaattct tttaatctat caagtgctct tttaataaca
    23461 taatccgttt tagatagtat tctaatatct atatgagaag gattatcaat aagtttaccc
    23521 catattagaa ctctgtttgc tatacaactg gatttacgaa tttttggaag ttctataaaa
    23581 tataatgcag ttttactaga aaaaatatct ggatgcccgt ccattgttaa tctcaggcaa
    23641 tttacataat cttctatgtg attatatttt acatgattta gtatatgtat tccaacagtt
    23701 tttccaattt ctttatattc tttaccttct tctaactttc taccatgtac acgactaaga
    23761 taatattcag ctctttccaa ataatcctct tcaagagctc tttgcatctc aatatttata
    23821 tggttacgtt cttcatcttc agccaaaata tcaagtctag gtcttttctc accaatgtaa
    23881 ttaattgttg ggtcggtttc aaccttaatt attcttttta tttgagtgaa ctgtttgata
    23941 ttcctattag catatggagc atctttgagg atatcattta taaaatttgc aagtaaatcg
    24001 taatcattat ttaaagtaaa caatgattta aatacctcat catactcagg cttagcatac
    24061 ccatcttcag ttacaacttc tccaacattt tcactagtca atatttcttt tggaattaaa
    24121 tccttgattt tatctaattt ttcattagaa aaatcacctc tactaagtat gtcatttaat
    24181 ttttgggtcc attctctgtc cttttcttca attatagttc gtttatactc ttttgatgtt
    24241 ttaataatct tagatttttc ttctaatttt tctttcatat caacacctct tttttattat
    24301 tacccatttc ttcataccaa agtctgaact tctaagtaaa atttgcatta ttgaaataag
    24361 aaccttataa acttatatat cattactaaa gttaagactt taaagagaag cacttagatt
    24421 acaatagatt ttagatagct ttctttttaa aactattgtt gataaatgaa tgaatataag
    24481 ggtatgtatt aaaatagaga tactcatctt tttcattgtt taagtaatga aaatatattt
    24541 tatttaatac aaaaaagagt gccttataaa ctaaaagttc acttcgagac actcccttac
    24601 attttgtttc ttaaacttta caatattaca tacttattta ttaatttaat ctatgcatac
    24661 ttaacccaat aataaagtac atatatccaa ctcattaagc catgaaaaat agcccatgga
    24721 atagatttcc atgcagtata agaaataatc attgctaaac aagaaccaaa agaaataccc
    24781 tttttaacag ttgattttat agtattatta tttgaatcca taattaatcc tccaaaattc
    24841 aaaataaaca agttattata tatcttattt gaatctaata caatataaca gcttctaaat
    24901 aaattatact cttattatac ataatttata aattataatt ccgataatta tatgaattaa
    24961 ttaattatat taacctaaaa atattatcct atctaaaagt tatatgatag atacaacatg
    25021 tcaacacatt gtaattctcc ttcaaagatt ggttctggat aattatctac aaaaaagttt
    25081 ttaattttat gtgaataaac aaatccaaac ttctcataaa aaggaactcc actttcagat
    25141 gtaccaacaa ccattgatga atatttttta ctataaacat ctaaaagatg ataaatcatc
    25201 tttgaggcat aacccatatt ttgaaaatgc tcatatgtag caatgttttt taattcacat
    25261 atttctttgg aaatttcatt tacaactgct atacaagtaa gttctttatt atcatacaat
    25321 gcgtacatag ttcctttatt gagatattta tcaatcattt cttcacatgg gtcagctaat
    25381 attaaaaggt caatttgact ttttttattt atttctatag tttttatttc tatcataagt
    25441 tctccaccct cactttattc aaaccttaat ttaacaaact tcttacagta cttatttagt
    25501 tcattttgat atatattttt actttttaaa atatagtgat aattccctag aatattaata
    25561 ttaataataa attataatat cagctctagc aattacctag aatatcaata ctaatattca
    25621 tctataatat tag
//
LOCUS       DAMJWU010000044        25600 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMJWU010000044 DAMJWU010000000
VERSION     DAMJWU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.044"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(248..964)
                     /gene="dapD"
                     /locus_tag="PC460_002051"
     CDS             complement(248..964)
                     /gene="dapD"
                     /locus_tag="PC460_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF2600935.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1188..1937)
                     /locus_tag="PC460_002052"
     CDS             complement(1188..1937)
                     /locus_tag="PC460_002052"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2600936.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(2004..2891)
                     /locus_tag="PC460_002053"
     CDS             complement(2004..2891)
                     /locus_tag="PC460_002053"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2600937.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(3008..4009)
                     /locus_tag="PC460_002054"
     CDS             complement(3008..4009)
                     /locus_tag="PC460_002054"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF2600938.1"
                     /translation="MKKVNVAIIGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4434..5315)
                     /locus_tag="PC460_002055"
     CDS             complement(4434..5315)
                     /locus_tag="PC460_002055"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2600939.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGIISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            5638..6831
                     /locus_tag="PC460_002056"
     CDS             5638..6831
                     /locus_tag="PC460_002056"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF2600940.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            7037..8155
                     /locus_tag="PC460_002057"
     CDS             7037..8155
                     /locus_tag="PC460_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF2600941.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            8286..8531
                     /locus_tag="PC460_002058"
     CDS             8286..8531
                     /locus_tag="PC460_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF2600942.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            8518..9264
                     /locus_tag="PC460_002059"
     CDS             8518..9264
                     /locus_tag="PC460_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2600943.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKSEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            9549..10424
                     /gene="hslO"
                     /locus_tag="PC460_002060"
     CDS             9549..10424
                     /gene="hslO"
                     /locus_tag="PC460_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF2600944.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            10815..11615
                     /locus_tag="PC460_002061"
     CDS             10815..11615
                     /locus_tag="PC460_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2600945.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            12142..14382
                     /locus_tag="PC460_002062"
     CDS             12142..14382
                     /locus_tag="PC460_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2600946.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            14796..16331
                     /locus_tag="PC460_002063"
     CDS             14796..16331
                     /locus_tag="PC460_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2600947.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            16347..17108
                     /locus_tag="PC460_002064"
     CDS             16347..17108
                     /locus_tag="PC460_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600948.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            17446..18315
                     /locus_tag="PC460_002065"
     CDS             17446..18315
                     /locus_tag="PC460_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2600949.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            18644..18895
                     /locus_tag="PC460_002066"
     CDS             18644..18895
                     /locus_tag="PC460_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF2600950.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            19007..19786
                     /locus_tag="PC460_002067"
     CDS             19007..19786
                     /locus_tag="PC460_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2600951.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(19921..20571)
                     /locus_tag="PC460_002068"
     CDS             complement(19921..20571)
                     /locus_tag="PC460_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600952.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(20568..21071)
                     /locus_tag="PC460_002069"
     CDS             complement(20568..21071)
                     /locus_tag="PC460_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2600953.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(21120..21635)
                     /locus_tag="PC460_002070"
     CDS             complement(21120..21635)
                     /locus_tag="PC460_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2600954.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            21915..22439
                     /locus_tag="PC460_002071"
     CDS             21915..22439
                     /locus_tag="PC460_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2600955.1"
                     /translation="MHEQIDCCERKKRTIDIKGENMRELLKWVSIINRYNTNYIDRKL
                     ADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVK
                     DKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMN
                     EEAKLEAKENGRKI"
     gene            22423..23826
                     /locus_tag="PC460_002072"
     CDS             22423..23826
                     /locus_tag="PC460_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2600956.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(24390..24701)
                     /locus_tag="PC460_002073"
     CDS             complement(24390..24701)
                     /locus_tag="PC460_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600957.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(24757..25350)
                     /locus_tag="PC460_002074"
     CDS             complement(24757..25350)
                     /locus_tag="PC460_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2600958.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         9506 a         3147 c         4226 g         8721 t
ORIGIN      
        1 gtttaatagt ttattacatt gatatattga aaaattgaag gaaataaaaa tacttgtcag
       61 caagaaataa aaatgatata agaatgaata agagaattgt aaaaaaataa aatgattata
      121 aataaaagat aaaaatattg taaaaaagag ggtgcctctg gtgaattaaa ttcacacaga
      181 gacacctttt taattaattt tttaaattag tatattattg tttaattaat agcatagtac
      241 tatttaatta atctaaattt ctaaggtctt ccataagctt agttttgtca gccgtttttt
      301 catctttcat ttttataact ttagcaggag aaccagcaac aacggcacca gcttctacat
      361 cagtagttac aacagcacca gctgcaacaa cagcaccttt accgattcta acaccttcaa
      421 gaataactgc attagctcct attaaaacat catcttcaac tataacagga gtagcagaag
      481 gtggctctaa aacaccagca acaacagcac cagcacctaa gtgaacattt ttaccaagag
      541 ttcctctagc tccaataaca gcattcatat ctaccataga accttcacca ataacagctc
      601 ctatatttat aacagctccc atcattacaa cagcattttt ttctatagta accatgtctc
      661 ttattattgc acctggctca attcttgcat gttcacttaa agtatttttt agaggaatag
      721 cagaatttct tctatcatat tctacatgag tatctgttat atcatttcca tgtttatcta
      781 atacttccat tatatcctca tagtcaccta caaatatata agaaccatta cttccaaata
      841 ccttaaattt atcagttgat tggaagtgta aagagttagt atttacatat accttaacag
      901 gtgttttctt tttaacttct tttatatacc ttgctatttc ataagcatcg tttaaattta
      961 ccatttgtta attctccttg tatttcatta tatttctttt attatatact tatttgtcaa
     1021 ttttagtata tacttacttc ttattattta tattttattt tagttagctt ttattgtata
     1081 gacatagtat taaataaatt tattatatat gtttgatttg tattgcatat tattatatat
     1141 atattgtata tgtttaattt atattgtgta ttattatatt tatactatta tcccaacatg
     1201 ctgtccatat tataaaatcc tggtttttgc ttaactaagt atttggcagc agctatagaa
     1261 ccacttgcaa agataccacg agattgagct tgatggctta ttgtaagaac ctcattgtct
     1321 ccagcaaata gagcatcatg ttcgccaaca atatttccac ctcttatagc atgaatacca
     1381 acttcatttg gttgtctttt tgcactacga ccatatctac cataattatt ttcaacgttt
     1441 ggcaaaacct cttttatagc gttagcaatc ataacagctg tgccacttgg ggcatctact
     1501 tttctgttgt gatgtttttc tattatttct atatcaaatc cttctagtaa tttagctgca
     1561 tctttaacca attttactaa tacatttaca ccaagagaca tattatatga ttggaataca
     1621 ggaataactt tagccgcttc acgtattttg ttcatttcgt catcattata tccagtagta
     1681 gcaattacta aaggtgtttt agtctttaaa acgtaagata agatattatc taaattagag
     1741 tgatgagaaa aatctataac tacatcagct tcctcaacta tagtactcat atcttcataa
     1801 attttaaggt cgccttcata gctatccaga cctctagctg caacacaaac caattctaat
     1861 tcattatctt ctcttatagt atcggctaaa atcttaccca tttttccatc ataaccagtt
     1921 gctataactt ttaacataat ttcctcctat gattaagggc taacttttga ccaaacttac
     1981 tttttaccat gcattaatct ttactatgca ttaactttta gtccaaagtt aactaattct
     2041 ttctttaata cttctaaatt tgctgggtcc atttctgcta gaggtaatct taaatctcca
     2101 acattgaatc ccaataaatt cattgcagtc ttaactggta taggatttac ttctataaat
     2161 aaagctgcga ttagagcatc cataccaagt tgtaattttc tagaaccttc aatatcgcct
     2221 tcaaagaatt tagctaccaa atcgtgagtt tcttgagggc aaacattagc aagtactgat
     2281 ataactcctt gacctcctaa agataataaa ggaagtatag tatcatcatt accagagtag
     2341 atagcaaagt catctggaac taaactagct atttctgcaa cctgtcctag gtctccacta
     2401 gcttctttta cagcaactat gttatcaatt ttagcaagtt caactataag gctaggactt
     2461 atatttacac aagttcttcc agggacattg taaagtataa ttggaagttt aacactgtta
     2521 gctattgttt caaaatgacg cttaagtcca gctttattag ctttattgta ataaggtgtt
     2581 attattaata gagcatctac tcctaatttt tcagcttctt gacttaagtt aactgagtgc
     2641 atagtatcat tagaaccact tccagctata acaggaactc ttttattaac tttttcaaca
     2701 acatacttaa tagtagcaag ttgttcttca tcagtcatag tattagcttc accagtagta
     2761 ccacaagata ctatagcatc agtaccatta gctatttggt attcaactaa ttcacctaat
     2821 ttgtcaaaat caactttgtt atcctttgta aatggtgtaa ctagagcaac agcagaacct
     2881 ttaaatatca tataaaatca atcccctctt ttttattttt gtgttttata ttacagttac
     2941 ttctaatgct tgtttttgag ttactattaa tattttacaa aattatatat ttatacaagt
     3001 ttgtatatta tactaaatcg tatgataaag ccatttcagc tatttgaatt gcgttagttg
     3061 cagcaccttt tcttatatta tcagcaacaa cccaaagatt tacaccatta tcaacactaa
     3121 aatctcttct tattcttcca acaaatactt catctctatc atcaaattct acagcagttg
     3181 gatattcgtt tttagcagga ttatcaacta ctacaacacc tggagcttct tcaagtgttt
     3241 ttactaaatc atctaactca aatggattgt taaactcaac atttatagat tcactatgac
     3301 cattttttac tggaactctt acagtagttg cagttatttt taaatcgtag ttatcaagta
     3361 ttttcatagt ttcatttacc attttcattt cttctttagt gtatccattt tcagtgaatg
     3421 aatctatatg aggaagacag ttgtaagaga tttgatgagg gtagaatcca gtaggattac
     3481 cgttcattcc tttctctaaa tcttcaacac ctttaacacc agaaccagat acagcttggt
     3541 aagtagagta aacaactctc ttaattttgt atttatcatc aagagcttta aggggaacca
     3601 tagcttgtat agtagagcag ttaggattag ctattatacc cttatgattt tttatggctt
     3661 ctggattaac ctcaggaact actaaaggca catctggatt cattctccat tgactagagt
     3721 tatctactac taagattcca tttgaagcag ctattggagc atacttttca ctaatacttc
     3781 caccagcaga gaataatgct atttgtatgc ctctatcagt aaatgaagtt tcctttaatt
     3841 cttctactat atattcttta ccacaaaaca ttactttaga accagctgat ttagaagaag
     3901 agaataaaaa tagattttct attggaaaat ttctttcttc taatactttt aaaaatgttc
     3961 tacctaccat tccagtagca ccaattattg ctacatttac ttttttcata taaattcacc
     4021 ccaattttaa attattaaaa cattatttta ttttagttat acaagtaaga tatgttaata
     4081 tttttatata tccaaaaact ttaatattat attaacaact ttaattatat acaaaaaacg
     4141 tacaaaggta tactttgtac gtctaattta tcactcaaag taaaccctgt agctcatcac
     4201 ctacaaaaat aagtgacagt attacacatg ttatgtgcaa tcccagaaaa tattattgca
     4261 agaaatattt tcttcggctg taaatccttt ttccaatcat taacacctgc cagctactat
     4321 tggatactct tagttacagc tacctctacc ctaggttaat aggatattaa aatttgatta
     4381 ttctaataat aatacatatt taagaaaaaa tcaacttatt tatataaata ttgctaaaat
     4441 ttaaatccaa aatttgtcaa ttcttgtttt aacacattaa gattagtttc ttccatttca
     4501 gctagaggaa gtcttaaatc accaacatta aaacctaaaa tgttcatggc agttttaaca
     4561 ggaactggat ttacttctat aaataaagca gcaattaaag catccatatc aagttgtaat
     4621 ttttttgagc cttcaatatc accttcaaag aatttagtta ctaaatcatg tgtttcttta
     4681 ggacatatgt tagcaagtac agagataata ccacttccac ctaatgataa tagtggtagt
     4741 atagtatcat cattaccgga gtatatagca aagtctttag gtactaattt agctatttct
     4801 gcaacttgag ctaagtctcc acttgcttcc ttcacagcaa ctatattatc tattttagca
     4861 agttcagcca caacagatgg ctttatattc actttagttc ttcctggtac gttgtataaa
     4921 ataattggca acttaacgct atttgctatt gtttcaaagt gaagtttaag acctttctca
     4981 ttagtcttat tatagtaagg agttattatt aaaagaccat ccacccctaa tttttcagct
     5041 tcttgactca tatgaacaga caacatagta ttgtttgaac cagtacctgc tataacaggt
     5101 attctcttat taactttttc aactgtatat tttattacag caaaaatttc agattcactc
     5161 atagtagtcg cctcacctgt tgttccacaa acaactagtg catctgtgcc attctcaatg
     5221 tgatattcga ttaattcacc taatttttca aagttgacat tattatcctc tgtaaatggg
     5281 gtaactaaag caacagcaga acctttaaat aacatataat caaatctcct tttttatcta
     5341 aatttaattt tttaagtaca ataataatta tagtacaaat taattaattt taataggtat
     5401 agttaatatc ttaactgttt aaataagata tttatattat attaatatta gaaaaatttt
     5461 aaataaatgt tttattctca aaatatgcat attaatttaa taaaatatac caaaatggaa
     5521 ataaaaaata aattttatga gaatttaata actaataatt aatatattta ggttataata
     5581 aatatgtgcg taaaatctaa caataagcaa taataccgta aatttaggag gaataatatg
     5641 gatactttaa aggaactttt taaaatagga agtggacctt caagttctca tactatggga
     5701 ccacaaagag cagcagagag atttaagaat gaaaacccag atgcagaaag ttttagagca
     5761 atattatatg gtagtttggc agctacaggg aaaggtcact taactgacta tataattgaa
     5821 aaaactatag cacctaaaaa agtcgaaatt gtttgggaag aagatattat aaaagatttt
     5881 cacccaaatg ggatgaagtt tgaagcttta gataaagata aaaatgtaac agctgaatgg
     5941 actgtatatt ctgtaggtgg aggaactata gcagaagaag gtcaaagaaa cagtaaaagc
     6001 aatagtatat atcacttaga tactatggat gagatagtaa aatggtgcaa agaaaataat
     6061 aagactttgg tagattttgt gttggaatgt gaaccaaaag acataaaaga ttatataaaa
     6121 actataaaag atgctatgag aaaatctatt gatgatggac taagtacaga tgaaataata
     6181 ccaggcaagt tattattaaa aagaagagca agtacatttt ataatgcata taaaaaagat
     6241 aagagcttct caacacttgt atatgcatat gcactagcag cttcagagca aaacgcttct
     6301 ggtaatataa tagttacagc accaacttgt ggttctgctg gagtaatacc aggaatattc
     6361 tttgcaatgc aggatttcta taattatgat gacgaaaaaa taattgaagc actacttgta
     6421 gctggaataa taggtaatat aataaaaact aacgcttcaa tatctggagc agaggtaggg
     6481 tgtcaaggtg aagttggagc cgcttgttct atggcagcag cagcagtagc atacttaaaa
     6541 ggtggaacta tagaccatat tgaatatgcg gcagaaatag cacttgaaca tcatttagga
     6601 atgacttgtg accctgtata tggatatgtt caaattccat gtatagaaag aaatgcaatg
     6661 gcagctcaaa gagcttatga tgcagctaat tatgcattat taactgatgg ctcacattct
     6721 gtctccttag accaagttgt tgaaacaatg aaagaaactg gtatagatat gatggataag
     6781 tacaaagaaa cagctaaggg tggacttgct aaacatttct tctcttgcta aaaataaaat
     6841 tttccaataa taattcctct gttttgattt atgaaacagg ggaattatta ttaatggtga
     6901 attatttaat aatgcaaaat atttttatat tcaatttagc tttttatttt taattagtat
     6961 aacatgctat ataatatttt aattataaag aaatatagaa atatttttta ataaattaga
     7021 aattggagga gattacatga gagaggcatc atatttacaa gaacaacttt ataaacatag
     7081 gaagtcatta aatcctattg cagagattgg gagaaaagag tataagactt caaaatatat
     7141 aagagattat cttgataaat taggaattga gtatgaagtt tatttagaaa caggtatagt
     7201 aggtgtaata gaaggtaaaa gacctaaaaa agatattgct tttagagctg acatagatgg
     7261 tcttcatagt gaagaagggg taaaacattt atgtggtcat gatggacatg ctactatctt
     7321 acttggatta attgagtatt taaatgataa taaagaagac ttaaatgata atatagtatt
     7381 tatattccaa ccggcagaag aaggtcctgg gggagcaaaa gctttaatag atgaaggagt
     7441 gcttagaaaa tacaatattg atgaaatata tgggcttcat atatatccag agttaccaga
     7501 aggttatgta ggtacaagag agggctattt tatggctcaa ataggagata ttgacataga
     7561 aatagtgtct aaaagtggac atggggctat gccacaaaat ggtattgatg gtatagtgat
     7621 agcttcaaac tttgtaagct cacttcaaac tatagtgtct agaaatataa gcccaataga
     7681 caatgcagtg ttgactattg gcagaattga agggggagct agaagaaata taatagctga
     7741 aaatataaag atggaaggta ctatgagatg tttcagccca gaagtgtatg agaaaatgaa
     7801 gcttagagtt agagaacttg ctagagggtt tgaattggct tataactgta aggttaatat
     7861 aaatattata gatgattata tcgctgttaa aaatgacaag gatttgtatc aagagtttgt
     7921 agaggctatt ggaagtgata ctgttgtaga gttagagcca cttatgatat cagaagattt
     7981 ttcttattat caaaaagaag ttccaggatt attttttatg ttgggttcaa gaaacgaaga
     8041 aaaaggattt gtaaatggtc ttcataatat aaactttaat tttgatgaaa agatatgtgt
     8101 aaatgcattg aatgtttatt caaaattatt aaaatacaaa gaagcaatag attaggaaga
     8161 taagttataa agattaaaaa gcataaatac ctaaatttaa aaaaagtgta ttaataaata
     8221 ccccttacat atttgtataa atttatttta attgacaaaa atacaaataa gaaggggtga
     8281 tttttttgaa agaaaaagta caaagagaaa agaagataac taatggcaag aatactaaaa
     8341 tattgacaaa tgatgatata atgaaatatg agatagcctc agaactaggt cttatggata
     8401 aagttggtga acgtggatgg gcaggactta cagctaagga agcaggaaga attggaggta
     8461 tgcttacatc acgcaaaaag aggcttaaaa agattgtgga ggagcagaat aaaaaggatg
     8521 agtcaatata gtgattttgc gtttgtatat gatgaactta tgaatgaagt tgattataat
     8581 ggttgggtta aatatataga agatataata gatagtgaag gtgtaaaagt aaaaaatata
     8641 ttggaattgg catgtggcac gggtaatctt acaattccat tgactaaaaa aaattacgat
     8701 atagctggta tagatatatc tgatgagatg cttagtgtag caagagaaaa gtcagaaaaa
     8761 gaaggagtag aattagtttt acttcaacaa gatatatcag agcttgattt tgaaatatct
     8821 gatttggact gtgtactttg tgcttgtgat ggattcaatt atataactta tgatgatgat
     8881 ttggaaaatg tattttcaaa gacatatgaa ttattaaaga aagatggaat attcatattt
     8941 gatataagtt ctttttataa attagccaac atattgggaa ataacatgta tggagaaaat
     9001 agagaagata tagcgtatat gtggcaaaat tactttgatg aagaagagaa tctaattgaa
     9061 atggaactta cattctttat taaagatgaa tgtggaaaat ttgaaagatt tgaagagata
     9121 caccaacaaa gagcatatac tgaagatgag atagttgata tgcttgaaaa atctggattt
     9181 aaggatataa aatattatgg ggattttaca tttgacaaac ctagtgaaga aagtcaaaga
     9241 atcttctttg tatgtaaaaa ataagatatt tttgactata taagataatt attaaaaata
     9301 accatgatat aaaaatatag aaaatgcagt tttatagatt aaaagtaaag tgatgatact
     9361 ttatgacttt tattttttaa taattataaa gttctataga taaaaattaa aatataataa
     9421 ctattgctta gtaaaattaa aataaaatgg gttttgccca aggttaagat agataagtat
     9481 ttatagatta aaattaaggt agtaatataa taaaaataag acaattaacg ggacatggag
     9541 gaatattaat gagagattat gtactaagag cgacatcagg aaatggtcaa gttagagcat
     9601 ttgtcgcaac tactagaaat acagtagaag aagctagaag attacatgaa actacaaaag
     9661 tagcaacagc agcacttgga agaacattaa cagccacatc tataatggga ttaatgatga
     9721 aaaatgatag tgataaattg actgtaataa taaaaggtgg aggtccaatt ggaactatta
     9781 tcgccacatc agactctaag ggaatggtaa aaggatatgt aggaaatcca caagtagagg
     9841 ttgaagacta tccaaatgga aagttaaatg tagcagcagc agtaggtaca gaaggtgttg
     9901 taaaagttat aaaagactta ggtcttagag aaccatataa tgggacttat ccattggtaa
     9961 gtggagaaat tgcagaagac tttacttatt attttgctgt atcagagcag actccatcag
    10021 tagtagcttt aggtgttctt acaaaagaag atgaagtaga gtttgctgga ggatttatag
    10081 ttcaattaat gccagatgca gaagaagaga ctattgctaa gttagaggaa aatgtagcta
    10141 aattaccttc tataactaac atgttaaaag aaggaaaatc accagaggat atattaaata
    10201 ttgttttgga tggtttggaa cctaaaatac ttgatacctg tgaagttggg tttatgtgtg
    10261 aatgttcaaa agaaagagta aaaactgctt tggttgcaat tggtaaaaaa tcattggctc
    10321 aaataataga agaagataaa aaagcagaag ttggttgcca gttctgtaat aagaaatata
    10381 tgtatagtga agaagagttg cttgaaattt taaaggaaat gtagactata aaaatagttg
    10441 tattgatata agtaatgtta tagtgatata gtaaagtcgt attgatatag taattaaagt
    10501 ataatttatt aataatagac atatttatat taaaaaataa gtttaataaa tttttaagct
    10561 taataaattt taagaattaa gaagctttct ggctgtaatg tttgcaatgt tgcagttaga
    10621 aggctttttt attttattat agttcgtgtc aaagattgta tgaaacaata aaatattgta
    10681 aaataatttg tatatttaga cctgattggc acaaaggttt ataaggtatt tgagttgatt
    10741 atgcgaaatt ctatgaagta gtaaccctgt gactttaatc atgggaggtt cagaagaagg
    10801 agaaaatttt aaatatggaa agtaaaacga agtacatagg aataagtata ttaatattat
    10861 tgatgatatt tttaattgca aaaaatttta acggaaaact tgaaatacct attcctgaga
    10921 gtaatagtag aaaatttaaa tcagcagcag tgttttatcc tcaacatcaa gatgatgagg
    10981 ttttatgggg aggaagtgcc attgtagatg ctataaaaca gtgtggtgtt gataatgtat
    11041 atgtggtatt agtatcagat ggttctggag ttaacgtttt caaagctaac acaaaattta
    11101 gaaatttaac taggaagcaa aaggaagaac ttagaaataa cgagtttaaa tcagctttga
    11161 gagagcttgg agtgaaacct caaaatgtga ttatacttgc agatatagat aaaaaggaag
    11221 gcacacatta tgaattaatg gaaaagacta tattggattt tgaacataag tttaaaggca
    11281 atgtgaccca tatagcccat cactataagt atgatgacca tataatgcat agaaagaatg
    11341 gggaagtttt aaagaaactt aaacgtgaag gtaagataaa agatgacttg tattttgtta
    11401 agccaaaata tgtaaagtat ataccaagaa atgaaagagc tatctataaa gcaatggatt
    11461 ttgatgacta caataaggtt aagagtgctt gttatgaata taaggttgta aattcaaagc
    11521 gtaaaatgta tggtatagga tatatttcgg cacatagtta ttttgaacat cttctaaatg
    11581 accctgaact tacgtctgta ttaagtgaag attaatttct tataagtagt aagttatgtt
    11641 tgataagttg attggaattt tctatagttt tgaaggtatt ggaaatttag ttttatttaa
    11701 agatttaaag atagaggttt ttagtgttta aaattttaaa gttaagttga ataattgtat
    11761 agaattattt gtgaaagaag aaacagtgta aaacatagta tcttatttat ggttttaaaa
    11821 tcataggcaa gatacttttt ttatgtaact aataccattt tgagattatt aacttaagaa
    11881 ttttatataa tttaacactc atatataatg atgatattta ggtagaatag atgcaattac
    11941 gcttaaataa aataactgta ttttttataa aaatcaaacc ctacactata ctaccagata
    12001 aaatctacat gctataattt gacctgtgtt tataaaaaaa ggagtaatct aataaaatag
    12061 tatttcactt atgtataact aacaactatt attacgattt tctctaaata atttaaagaa
    12121 aaataatgca ggaggtaaaa tatgaacaat gaatttataa ttttaaaagg ttgcaaagag
    12181 aacaatttga acaatatttc cttaaaaata ccaaaaagaa aaataaccat attcacaggg
    12241 gtgtcgggtt ctggaaagtc atctattgta tttgagacaa ttgcaaaaga atcacaacgc
    12301 caactaaatg aaaggtttag tacatttgta agaagttttt tacctaagta tggagaggtt
    12361 aaagctgatt gtatagaaaa tttgtcaaca ccaattatca tagaccaaag tagacttggt
    12421 ggaaattcaa ggtcaaccct aggtactatt acagatgtaa attccttttt aagagcttta
    12481 tattcaaggt ttggtagtga gtatataggt aatgctaata tgttttcttt taataatatt
    12541 gatggtatgt gcccacactg tcatgggctt ggaaaaaagc tagttcctaa tatgaaccaa
    12601 attcttgata tgaataaatc attaaatgaa ggtgctatat tgctttcagg gtttggagtg
    12661 ggttcttggc attggaatat attcgctgaa tcaggatttt ttgatattaa taagaggata
    12721 tgtgattatt cagaagtaga attggaaaag tttttgcatg gtaaagcaga aaagattaag
    12781 atagaaaatg ctggtcaaac taacatgaca tatgagggat taatggttaa atttaatcgc
    12841 ctatacttaa gtagagaggg agaaatatct gaagctacta agaaaaaatt gagtaaactt
    12901 cttatagaag atagatgtcc tatatgtgat ggaagaagac ttaatgaaag agtatatagg
    12961 tctttaatta atggttataa tatagcagat ttaactagta tgcaaattga tgaattggct
    13021 gaagttatta aaagtataga tgagccagaa gcacaacctt taattaaagg aataatagaa
    13081 aagttaaaca gtatcataga tataggtctt ggatatttaa cactggatag agagacatct
    13141 tctctatcag gaggggaatc tcagcgtata aagatggtta agtatttaaa tagtaatctt
    13201 gttgatttaa tgtatatttt tgatgaacca agtgtaggac ttcatccaaa agatgtgtat
    13261 aaattaaata atcttttaaa aaaattgcgt gataaaggga atacaattat agttgttgaa
    13321 catgacccag atgttattaa gattgcagac catattgtag atgtaggacc aaaggcaggt
    13381 aagtatggtg gagaagttgt gtatgaaggc agttatgaaa atctacttac aagtggtact
    13441 ttgacaggga atgctttaag taaatttctt cctattaaag aaagtgtaag agaacatagt
    13501 ggatatttag aagttaaaaa ttgtaataaa aacaatttaa aaaatgtatc gattaagata
    13561 cctaaaggtg tattgactct agttactgga gtagcaggag caggtaaaag tacgttgatt
    13621 aaggatgaat ttttaaagca aaatcctagt gctgtactta tagaccaaag ccctgtttca
    13681 gcaaattcaa ggtcgagttt ggcaacatat agtggaatta tgaacaacat aagaagcata
    13741 ttttcaaaaa caaatggcgt aaatgcttct ttattcagtt caaattcaga aggagcttgt
    13801 gataactgta agggttctgg aatagtagaa atgaatcttg cttttatgga gagtataaag
    13861 agtacctgca atgtttgtga aggtaaaaaa tttaaaaaag aagttttaga atataggttt
    13921 caagggaaga atataattga agttcttgaa atgtctgttt tagaggcaat tgagtttttt
    13981 aatttaaaac agattaaaac gaaattacaa tgtattgaaa aaatgggtat tggatacctt
    14041 actttaggac aaactcttga tactttgtct ggaggagagt gtcaaagatt aaagcttgct
    14101 agtgaattaa ataaggaaag ttcagtgtat atattagatg aaccaacaac aggtcttcat
    14161 atggctgata ttgagaattt tataagtata attgaagaga ttgtagataa tggaaatacg
    14221 gtcataataa ttgaacacaa tatagatgta attaaaagag ctgattggat tattgagctg
    14281 ggacctgaag gaggaacaaa aggcgggaga gtaatatttg aaggtattcc aaaacaatta
    14341 tgtaacaata aaatatcatt aacagcaaaa tatatagtat aataaataaa atataataaa
    14401 attataatga tattttaata aatataatgg gtatataaaa tgataattaa acttaacagt
    14461 aataaataat gtgagaaata ttcaaattta attaaataaa gtgtcagaaa tttgtttaaa
    14521 ttttagtatt ttaacggttc tgaccttttt tcttattttt atggagaata tcgaggatgg
    14581 agtctctaat agttactttt ttgtgtctac ttgcaagttt gattaaaaaa tggctataca
    14641 cacttgtgac ttcagtcatg ggaggttcag attataatct taatatataa tttgtgaaat
    14701 aatttgatga cgatttgctg atgtataaac aataaagatt aactacatta aaatttaaat
    14761 cttgttaagt aaaacttata aaaggagaga gatggatgat aaatgatatt ctaaccttat
    14821 tatctgaaag aagtgatttt tttataaagc tatccataga acatttgatg atttctatta
    14881 tttctataat aattgcgatt gttttgggtg gtataattgg tattattgta agtgaatttc
    14941 ataattcttc taaacctata ttgggaacta tcaattttct atacaccatt ccatctattt
    15001 ctatgctagg atttctgata ccattttccg gaattggaaa tactactgct gttattgcac
    15061 ttaccattta tgcattattg ccaatggtta gaaatacata tactggaatt actaatgtgg
    15121 ataaaaatat tcttgaagca gctaggggaa tgggaagtac agattttcag atattgtata
    15181 aaataaaact tccgttagcc ctacctgtta ttatgtctgg tattagaaat atggtgatta
    15241 tgacaattgc attagctggt atcgcttcat ttataggtgc tggaggtcta ggtgtagcca
    15301 tatatagagg aatcacgaca aataacacaa taatgacaat tactggaagt atacttattg
    15361 ctctattagc tttaatattt gattttattt tagggctggt agaaaaattt tttcaacgaa
    15421 aaagaaaaaa gaaaaatagg aaaaaagttt ttgccattct aggaagcctt attcttatag
    15481 caactatctt tacgtcttat ttatttaatc cttcaaagac tcaaactatt catattgcta
    15541 ctaaacctat gactgaacaa tatattcttg gtgagatgtt ggatatttta attgaaaaaa
    15601 acacaggtct aaacgtggaa attacacaag gtgtaggagg gggaacatct aatatccagc
    15661 cagctatgga gaatggagaa tttgacattt atccagagta tacaggtaca ggctggaata
    15721 tggtacttaa gaaaaatgaa ttatatactg aaagtatgtt tagtaaacta cagtcggaat
    15781 acaaaactaa acttaaaatg caatggactg gtatgtatgg tttttctaat agctatgcat
    15841 tagcagttag aaaagaagtt gcagataaat acaatttaaa aaatataacg gacttaaaag
    15901 atgtatcttc taaattgaca tttggagctg aatatgactt ttatgagaga gaggatggat
    15961 atactccatt gtgtaataca tatggactga actttaaaga tacagttgat ttagatattg
    16021 gattaaaata ccaagctatc aatgaaggta agattgacgt aatgcctata tttacaactg
    16081 atggtcagta tagtgtttca gatgttgtag ttctaaaaga tgataaaaat ttctacccat
    16141 cttatcaatg tggaaatgtt gttcgtttgg aagtattaga ggaaaatcca gaactatcaa
    16201 aggtatttaa gatggttgga gatattttga ctgataaaga aatggctaaa atgaattatg
    16261 acgtagaaaa caacaattta gaacctagag aagttgctcg tgagtttctg aataagaaag
    16321 gtcttttata aattggagga aatataatga cacctattat acaatttaaa aatattaaaa
    16381 aacaatataa tgataaaact ataatagata atcttaattt agatatagaa aaaggagagt
    16441 ttttgactgt aattggttcg tctggttctg gtaagacaac actcttaaaa atgattaatg
    16501 gtctgatttt gccagatgga ggaaatattt tgataaacca aacagatatc aaaaacgagg
    16561 acttaattaa attaagaaga agaataggat attgtgtaca gggaagtgtt ctttttcctc
    16621 atatgactgt tgaagaaaat atttcatatg taccaaatct cctaagtaaa aagaataaat
    16681 tagaagtaaa aagtgctgta aacaagtgga tggaaatagt gggattgcca aatgatatga
    16741 aagtgcgtta cccatcagaa ttgtcaggtg gtcagcaaca acgtgtaggg attgcaagag
    16801 ctttagcttc ttctccagaa attttattaa tggatgaacc ttttggtgca gttgatgaga
    16861 ttacacgaaa gcaattacaa aaagagataa aagaaattca taaaaaaaca ggagttacaa
    16921 ttatctttat tacgcatgat atttatgaag ctttgatttt gggaacaaag actttggtat
    16981 taaatcatgg agttgttcaa cagtatgata ctcctgagaa tattttaaat actcctgcta
    17041 accaatttgt agaccagtta ttgaatataa gaaaatctat aatagatgat gataatttaa
    17101 aagaataaat ttaaatttaa aatcaaattg ttgtgataaa aactattgat ttagagctaa
    17161 cttcaagttg tatacttgaa gtggaaaagt tagattaagg tatagaaggg catttagcag
    17221 ttggctgtag acgaggtaac tgctgaaggt gctttcaata aatggctaga acctgtatct
    17281 gttgaagatt ataataaatt gaaataagaa gctcaatata cagagtagat aggcacttca
    17341 aatagaagtg tcttttgtgt gccataaaat aaatcaaaat attagcaaag aatgtgaaaa
    17401 acaaaataaa ataatatgct atctttaaaa taggagggat gctatatgga taacaatttg
    17461 attaccattg aaaaaacttt agattatata gaaaaacatc ttttagatga gatgttagat
    17521 ttaaatgtaa tttctaaaga gataggctat tcaaaatacc atttacatag aatgtttaca
    17581 tcagttgtcg gatttagtgt acataattat atacaaagac gtagacttac tgaagcagcg
    17641 agaatgctaa tttttacaga aaaaccaatt attgagattg ctttaatctc ggggtatgat
    17701 acacaacgct ctttttcaag agggtttaaa aaaatgttta aacgtagtcc taattttttt
    17761 agaagacatc aagatttttt accactacaa ttaaaatttg atattcataa tagaaaaaaa
    17821 ttacgtggag atatggtttt agatgttaaa aaagttaatt gtgcagatat aaatattgtt
    17881 ggctattctg gtaaagtaag aaaaggattt catattattg gaaaatgctg gagggatttg
    17941 cataaaaata aagagatgat agaaaataag gtagatttag gttttttaat tggagttagt
    18001 gattatagca attttaaaga gattgagaat actcctacat ttacatacat tgcaggtgct
    18061 caagtagcta cttttggcaa attacctaat ggaatggaaa aattcgtatt accgaaaagt
    18121 aattatattg tttttacttt tattgggaaa aatgaggata gtatgcaaaa tgttgtagaa
    18181 tatatctatc aagaatggtt ccctaattca agttgccagt ttaatgaaaa taatttatat
    18241 gactttgtaa aatatggaga aaaaactgat gagaatggtt tgagtgaaat tcagttttgg
    18301 gttccaatca tttaaaagaa ggtattatgt ataaagtaga tggatttata taggcaaagt
    18361 tttacagcag tatgtattta taaataattt atagtttgta ggaaagaaat aattttacta
    18421 taagattatg ataaaataca caatactatg acaaaatagt gttaaataat agatttaatt
    18481 ttgcatagga aaagttctat aatggtgtgt attcataaat aatttataat ctgtaggaaa
    18541 gaaatagttt tactataaga ttatgataaa atacacaata ctatgataaa atagtactaa
    18601 ataatagatt taattttgca aaggataagg tgttttaata aaaatgaatg tagaaaacga
    18661 attacttaaa aacctagata gattgcatac aacagagtta ggcgttgtaa gaattaagaa
    18721 aaatctttct ttggagacaa atgatgtagt gaactggtgt aaaactaaaa tagaatctcc
    18781 aaatgccatt attaatagaa agggaaaaaa ctggtatata agtgtatatg attgtattat
    18841 aacagtaaac gctcacagtt atacaattat tacagctcat aaagaaaaga aatgaattgc
    18901 aaaatacaaa ggtcaaaagc attttccact gtttgtagta cctatgaaga agtatataaa
    18961 ataatattac ttttgaataa taatttaaat tttggaggtt gaaaaaatga atgatactgt
    19021 aaaagaaaaa ttggcagagt cactaacttc tgatactact gatattttac cttatttgcc
    19081 ttatttatta caagattttt gggaattagg aagttcacca gaacaaatgt tacaattatt
    19141 tagaaagcat atacctttta atgaggatac aaaagttttg gatttagctt gtggaaaagg
    19201 tgctgtatct gtgaagcttg caaaagagtt gaatatacat atcaaaggta ttgatttgat
    19261 acctgaattt attgattatg ctagaagaaa ggctattgaa tataaggttg atgctttgtg
    19321 tgattttgaa atgaatgatg ctaatagttc tgtaaaaatt gagcatgatt atgattgtgt
    19381 gatttttgga gctgtagggg atattttagg taattataaa gaaactataa gtaagctatt
    19441 acctacaata aaaagtaagg ggttcatttt aattgatgat gcatatgtag tagataaatc
    19501 tacaaatgaa cagcttaatt ttgagagggt gtatcccact tacaatgagt ggtgtatatt
    19561 gttcaaagaa ttaggtctga agctaattga atgtatacca tgtttagaac ttgaaaatga
    19621 tgttgaagag gaaatgaatt ggattataac tcgtgcaaat gagctaatta aaaaatatcc
    19681 ttctcaaaaa gatatgtttg aacaatatgt tattagccaa aaaaatgaat atactgattt
    19741 acagcaggac cttgttggag caacttggat tttacaaaaa gaataagata aatattagac
    19801 tagataatca atgttatagt gaattatccc atgtctagtg aatataaata acataaaaaa
    19861 ccgtcaagat gaatagttta ttcgtgcttg acggttttta gtatttgttg aagtaaaatt
    19921 ttatttcttt agtatatcac ttttagtcaa tccatttgat ttagcttgag tattccattg
    19981 atgattcccc ataagtttat ataggaatct agggaagtta ggtgaaataa aaatagtact
    20041 tttgttggaa tcatctatat gtatattatc actaagtata ttttttgaaa gttcttttaa
    20101 tgcaattcct aaatcttttt taggtccttt acccaaaggt acggattcta gtgaaccaaa
    20161 catctcccca gctccaattc caataccttg aacccatgta aagttgattt ttttacacca
    20221 atgctctatc atttgaattg ccaaatgatt atgttcacct tcattgaatc cacagttaac
    20281 tattgcatac actggaatag gttttttatt ttgtgttttt aaaaattttt cacatttctc
    20341 taaaaaatga agtaaatgag atggaatacc atctacataa agaggagatg cgattattaa
    20401 tctatcacaa ttaagtatgg cattaagagc gtcaggatac ttagatgtgt tatctacttt
    20461 aaatgtaaaa actttattat cattttgaat aagttgtttt aattcagata aaaaatactg
    20521 tgaattattt ttatttactt ttggactacc atttatcatt actatattca tatggattca
    20581 cctcttatat cttgtatatt ttcatagaaa caaacagagt gttcttttat attgaaattt
    20641 atagcatttc catttactaa tttcgtagca gtttcttttt cagcttctgt aatacaatct
    20701 ccatagaata tagcttttaa atttatctgt ttatcatatc tgcttttatg acgcatcata
    20761 ttatctttta ttacaaagta tggaagaata taattaatgc ttttatcaag tacgtttttg
    20821 acaaaagggc taaaccctcc gtaacagcat ctgcttatta taataattcc atcagagtca
    20881 tttataagtt tacccatgtt gctgtaataa tcttttacaa cacattcaga aggagtttta
    20941 atccagcaac caaaacaacc aatacatttg tggatttctt ttgtgtttgg aagtattttt
    21001 atattgtcat caagtttggg taataacttt tctaattctt tgtcagttaa atcatgtatt
    21061 aaaagcctca taaagtccat ctccttttat tatgtatgtt tacttctaaa aattatatct
    21121 tatagatttt tcagtaaatc tttacaccaa ttaataacaa aattataata gtcaactcca
    21181 taaatcattg ttgcatacat atatgtatat tggtcaacac aatgattttc ttctgcatca
    21241 tttttttgtt tacttaattt atttagaagt aattctactt cagttattaa catttttaag
    21301 tttgtttcaa ttatttcttt gggtagatac ttgtaaaaga atatattaac aaggtggtct
    21361 tgctttgttt tagaaaaatt tataggtttc tttaaccatt caagaaatac ttctttacca
    21421 gtattagtta tggaatatag tttttttaat ttgttaccat ctataacttc gtgatagtgt
    21481 atatatcctt ttgtttctaa tcttttaagt gcaggataga tacttccaaa gctggcgtca
    21541 aaaaagtacg aggtactttc tgacattatt tgttttaagt cgtatccact taattctttt
    21601 tccattaaaa atcctaaaat aatgtattct aacatattac ctcctggtat caattagata
    21661 tatatcgatt cgatatattt attattacag ataataaatc ttacgtcaag atgcttttta
    21721 agataagcta aaaagaatta ggagttattg ataaatatgc aaaaaaatta atataaaagg
    21781 taattaaata tcaataaaga tttaaaaata gttaaatcaa agtaagagtc taatatataa
    21841 aatttatagt aactttatgg tttaaatata aacttgtgtg ctattgacaa agtataaaat
    21901 tagcagtaga ataattgcat gagcaaatag attgttgcga acgcaaaaaa aggacaatag
    21961 atattaaagg agagaatatg agagagctat taaaatgggt ttccattata aatagataca
    22021 atacaaatta tattgacagg aaattggcgg atattggaat caataatagc caatattttt
    22081 atgtattaca catatgtaat aatccaggta ttacacagga cactatattt gagaaaattc
    22141 ttgtgaatcc tagtaatata acaagagctt tggcaaattt agaaaaagaa ggctttataa
    22201 caagagaacc aggtgtaaag gataaaagaa catggcattt atatcctaca gaaaaatctt
    22261 taggatgtta tgatgaaata ctaaaaataa caaatgggta tgttgaggag ttgttgagtt
    22321 catttgagaa agaagagcaa gagttgttta tgtcaatgtt aaagaaagtt gcatttttgg
    22381 ctattgatat gaatgaagaa gcaaaattgg aggcgaagga aaatggaaga aaaatataag
    22441 gagccattgg aatatgaaaa ggtttcaaag ttattaacta agtacgcaat tcctagtatt
    22501 atagcaatgt tagtaagtgc tttatataac attgttgacc aaatatttat tgggcaaggt
    22561 gttggtgtat tgggcaatgc tgctacaaat gtcacttttc cactaacaac aatatgtact
    22621 gcaattgcat tattgttagg aattggagga gcgtctaagt gttcattgga attaggtgct
    22681 aaaaattcag aaaaagctac aaaagcagca ggaaattcta tatgcttgat ggctgttttt
    22741 ggaataagtt tatttatagt agtatcaata tttttaacac cactattaaa attctttggc
    22801 tctacatcag aaatactacc ttatgcacac acatatacaa aaattacatc aattggatta
    22861 ccatttctta taatgtccac tggaatgagt aagttgatac ttgcagatgg gagaccgaaa
    22921 gcttctatgt tttgtatgtt aataggagca gttattaata caattttgaa tccattattc
    22981 atttttgttt ttaatatggg aattgctggg tctgctttag caactgttat tgggcaactt
    23041 atatcttttg gaataagtgt tcattatata aagaattttc aacatattaa attacataag
    23101 aaaagtttta aattagatag tataatttat aaacatatat ttgcgctagg ttcaagctca
    23161 tgttttaatc aattagcaat gactgtaaca cagatagtta tgaataatac attggcatat
    23221 tatggggcta ggtcagttta tggaagtgag attccacttg cttgtgtcgg tatcattatt
    23281 aaagtaaata tgatttttat atctataatt attggtatat cacaaggtat gcaaccaatt
    23341 atagggtata attatggggc tgaaaagtat aatcgtgtaa aagaagcata taagtttgca
    23401 gtgggaagtg caactattat atctgttatt gcattcttat gtttccaatt attcccaaga
    23461 caaattacgt ctatatttgg taacggaaat gagacttatt ttatatttgc agaacattat
    23521 tttagaactt tcttgttctt aacatttata aatggtgttc aaatagtatc atcaactttc
    23581 tttacatcaa ttgggaaatc agtgttggga ctttttactt ctttgactcg tcaaatttta
    23641 tttttaattc cattgatagt aattttacca atgtttatgg gaattgatgg agtaatgttt
    23701 gcaggaccaa tagcagatgc agcagcggca atggtatgta tatactttgc agttcatgaa
    23761 atgaaatcat tgactataaa gcaaaataat ttgatgagta accataataa gattgcagag
    23821 gtataagaca atatttaaga agtatagaaa attaatttaa agaagtataa taaactaatt
    23881 taggaagtat agaaaattaa tctaaagaag tataataaac taatttagaa agtatagaaa
    23941 attaatctaa agaagtgtaa taaactaatt taggaagtat agaaaattaa tctaaagaag
    24001 tgtaataaac taatttagga agtatagaaa attaatttaa agaagtataa taaactaatt
    24061 taggaagtat agaaaattaa tttaaagaag tataataaac taattcagga agtatagaaa
    24121 gctaatctaa agaagtatag taaactaatt taggaagtat agaaaattaa tctaaagaag
    24181 tataataaac taatttaaga aatatagaaa attaatttaa agaaatacaa tacattaatt
    24241 tttaagtaac attaaattaa tgtaagtaaa ataatgttca tatgaagatt taaaggtgca
    24301 ttttctgaag ctaagtatat gttgtagagt gttaggtctc tctatcctgt gaagtttaat
    24361 agctcaagaa aatgcactat ttatgatgat tattttgttt tatttccaat tttggaaata
    24421 actattctaa ttaaatctaa aattatacca taaataagaa aagaaagtat agatgctatg
    24481 taccaataaa tcgaaattga ccctgtaatt gttctacatg ggatttgcat acctgaatcc
    24541 ataaaaacaa aaaaactatc aactctggaa ataatagaca aaataggatt taaacctatt
    24601 agaacaatac tttttgagtc ttgaccaatt tgatgcatat aaatctcaaa taatgcaatt
    24661 aaaaaaaacc aaaatgtaaa agatttaata atttttttca tcaaaatacc tcacataata
    24721 taaattctgt ttgcactatt tgcttactac aactatttat ctccattcag tcacatcact
    24781 taattgtaat ctaggtcttc ttgatggttc ttcatcagga taacccaaag atattgctgc
    24841 cactaattga ctatctgtat ttaaccattc acataattca cgatatgcaa agtatacatc
    24901 acaaatccaa agactaccta gacctaattc cacagctgta agcgacatat tctgaattgc
    24961 agctccaatt gattgcatgt ttgccatttc atagaatcgt tcttcagggg atagcttttc
    25021 taaaggtgat ttacctaata tatttaaaat aaaaattgtt actggagctt gtttcatgat
    25081 ttcaactgtg taatttgcac cagcgatatg ttgacgactt cctggaagaa gcccattatc
    25141 attaatttca ttttgaatac ctttgctcat ggctttaagc atagattctt tttccttttc
    25201 agtgatgaca acaaaacgcc atggttgcct gtttttagat gaaggtgcat ttattccagc
    25261 ttcaattatt ttctctatag tttcatgtga aatgctttga tttttatatt ttctgatact
    25321 tcgtcttttt gatatagaat cacttatcat tttaagtaac ctccgtttat tttttggaaa
    25381 aatctattta atttttattt tctattttaa ttattttagc attaatcaga aaaaaagtca
    25441 ttgattttag tatacaatta ttaatgatta tattaagaat tgtatcaaaa agtatgtgat
    25501 tatactaagg gattatatta aacattatat ttaaaattgt atgaaaggtt gtatttaata
    25561 ttatattgag agatatatat attaggattt atatatattc
//
LOCUS       DAMJWU010000045        24557 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMJWU010000045 DAMJWU010000000
VERSION     DAMJWU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.045"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(476..652)
                     /locus_tag="PC460_002075"
     CDS             complement(476..652)
                     /locus_tag="PC460_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600959.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
     gene            1227..2249
                     /locus_tag="PC460_002076"
     CDS             1227..2249
                     /locus_tag="PC460_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600960.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALN
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            2263..3150
                     /locus_tag="PC460_002077"
     CDS             2263..3150
                     /locus_tag="PC460_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2600961.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRAGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            3156..3929
                     /locus_tag="PC460_002078"
     CDS             3156..3929
                     /locus_tag="PC460_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2600962.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            4041..4766
                     /locus_tag="PC460_002079"
     CDS             4041..4766
                     /locus_tag="PC460_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600963.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLFAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            4770..6173
                     /locus_tag="PC460_002080"
     CDS             4770..6173
                     /locus_tag="PC460_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2600964.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            6184..7008
                     /locus_tag="PC460_002081"
     CDS             6184..7008
                     /locus_tag="PC460_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF2600965.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(7333..7953)
                     /locus_tag="PC460_002082"
     CDS             complement(7333..7953)
                     /locus_tag="PC460_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2600966.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNKLIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            8219..8551
                     /locus_tag="PC460_002083"
     CDS             8219..8551
                     /locus_tag="PC460_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2600967.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            complement(8671..9312)
                     /gene="pcp"
                     /locus_tag="PC460_002084"
     CDS             complement(8671..9312)
                     /gene="pcp"
                     /locus_tag="PC460_002084"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF2600968.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(9333..10265)
                     /locus_tag="PC460_002085"
     CDS             complement(9333..10265)
                     /locus_tag="PC460_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF2600969.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            complement(10287..10952)
                     /locus_tag="PC460_002086"
     CDS             complement(10287..10952)
                     /locus_tag="PC460_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF2600970.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            complement(11522..11995)
                     /locus_tag="PC460_002087"
     CDS             complement(11522..11995)
                     /locus_tag="PC460_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2600971.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(11998..12402)
                     /locus_tag="PC460_002088"
     CDS             complement(11998..12402)
                     /locus_tag="PC460_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2600972.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(12377..13006)
                     /locus_tag="PC460_002089"
     CDS             complement(12377..13006)
                     /locus_tag="PC460_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2600973.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMYSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      13371..13414
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            13515..14483
                     /locus_tag="PC460_002090"
     CDS             13515..14483
                     /locus_tag="PC460_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF2600974.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            14485..15048
                     /locus_tag="PC460_002091"
     CDS             14485..15048
                     /locus_tag="PC460_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2600975.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            15056..15745
                     /locus_tag="PC460_002092"
     CDS             15056..15745
                     /locus_tag="PC460_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2600976.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            15872..16600
                     /locus_tag="PC460_002093"
     CDS             15872..16600
                     /locus_tag="PC460_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2600977.1"
                     /translation="MMFDRVKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            16614..17288
                     /locus_tag="PC460_002094"
     CDS             16614..17288
                     /locus_tag="PC460_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2600978.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYIFKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            17314..18150
                     /locus_tag="PC460_002095"
     CDS             17314..18150
                     /locus_tag="PC460_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600979.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            18166..18837
                     /locus_tag="PC460_002096"
     CDS             18166..18837
                     /locus_tag="PC460_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2600980.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            18827..20059
                     /locus_tag="PC460_002097"
     CDS             18827..20059
                     /locus_tag="PC460_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2600981.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            20209..20526
                     /gene="trxA"
                     /locus_tag="PC460_002098"
     CDS             20209..20526
                     /gene="trxA"
                     /locus_tag="PC460_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2600982.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            20543..21421
                     /locus_tag="PC460_002099"
     CDS             20543..21421
                     /locus_tag="PC460_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2600983.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            21691..22059
                     /locus_tag="PC460_002100"
     CDS             21691..22059
                     /locus_tag="PC460_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2600984.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            22062..22433
                     /locus_tag="PC460_002101"
     CDS             22062..22433
                     /locus_tag="PC460_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF2600985.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(22578..23033)
                     /locus_tag="PC460_002102"
     CDS             complement(22578..23033)
                     /locus_tag="PC460_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2600986.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(23072..24337)
                     /locus_tag="PC460_002103"
     CDS             complement(23072..24337)
                     /locus_tag="PC460_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF2600987.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         9488 a         2718 c         3782 g         8569 t
ORIGIN      
        1 aaaaaataaa aaacttatat aaataaaatg cttgtttttc ttgtattatt tatataaata
       61 ttgaaatttg tctagttata cgtttttatt gaccagttat gcagttgata ttgaatattc
      121 taaatattgg tgttatttta taggtggttt aaacatatta gggggatttt atatatgatt
      181 tgtatgattt atagagaatg tataaaatag taaaggttta gagccatgtt tagtggattg
      241 cagaatatgc atatagaatg attcttgtaa attaagttag acataggaaa tatataattt
      301 gtattggatg attaatttta tggtgtatgc ttttaacttc tataatggga aagtaaagat
      361 gtactcccaa tacatcttta ctttagcaga agttatagtt acttataaag tacaaaaaat
      421 tatatattta ttagagtaga taaatctaaa aaggtttttc tagaaaaatt tagttttaat
      481 tgtatctact tactaataat aagacttgat gtaagcaggt ttcaactacc gtttctccat
      541 attctgatat aaaatcatct aactgtgtat ctaaaaaatt acaataatca tatgaactac
      601 tgatgtaagc agttagtata tgtgtacatt tgtctatttc tccaataaac atattatcca
      661 tccccctaaa aaatttagta aaaataaaaa gtcgcattct attagtcaat taacataatt
      721 tataaaattt tttagtacta gtttctataa cttaactcct taaggttaaa tattcccagt
      781 tttaccaatt tagttttatt ctacaagtat ataatactag aagatgcttc attattcaac
      841 atgcttgttt gagaatagaa aatattctaa aattataatt gtttttaaaa ataagattca
      901 agataaataa agttatatgc aactatttaa taatctttcc ataataagga cagtaggttc
      961 tattcttaaa aaactccatc tttaaattta aagaataact acaatttgaa caacaataaa
     1021 atttccaatc aataaaaata tcaaaaataa taaaaataat tgaaaatgta taatttccca
     1081 gaaaaataat aaatgtaact ccaataataa acacacctat aaattataaa ataatttaag
     1141 ctttatttaa ggttagtatt atatttatat aagatttaag ttttaatata tatgtgtaat
     1201 taaaatgatt taggaggaga aatatattgg taaattggga aataaaaaaa ttggctaaat
     1261 ctaagagtat gtttatatct ctaggcatat tagtgttaat actaatgatt agtatcttta
     1321 ttacgcctgt tttagaaaca gaaagttatt atatagataa agaaaaagga cgtatagaag
     1381 atacgagaag tggtatcgac ataggtaatg agaaatttca aaataagatt aatgttttaa
     1441 agcagttttc aaatcaaaca gatacaggtg aattttctaa aaaaattgaa ttaatgtcaa
     1501 agaaaaaact tgataatcta agtgtgaatg aatataagga tattagtttt tggaaagtat
     1561 ttaactatag agttacaaat tcacttataa atgtaggaat gttaatcatt atatctataa
     1621 ttatatctaa tatttacgta gatgaagtcg ttacttcggt aaaagatatc atactatcat
     1681 caaaagagaa gaaaaaagca ttaaattcta aaatatttgt tgctttatta gttccattag
     1741 taatttattc tgtttattta atgggaatat ttattataac ttgtatgcaa caaggagtac
     1801 ctgttaatgg agacttagaa tcttttagaa tagtagacaa tgtagttgca ttgaaaacta
     1861 atttaagtat aacaaagtat gtaatattaa atataggcat agagcttttg atgtttgaag
     1921 gatgggccat gatagccata tttgggtctt ttataagtaa gtcttcaata tcttcaataa
     1981 gtttttttat atttattata tctataacta agataatgtc ggtaatccat atattaccta
     2041 aaaaattact ttcagttgta tcaaatgtaa attattatga tttgatattt ggttttaata
     2101 aaataatagg aaattatcta ggtgatatta tgatatttaa agtgaatata gacattgtca
     2161 atatagcaat aggacttttg atggcaatat tgataggtgc tatgtcatta tgttttttag
     2221 taataaaatc agattatata agtagataaa aggggagtta agatgaataa attagaaata
     2281 gaaaatcttt caaagatata tggtaaaaaa gtagcaaacg acaagattac agtaactcta
     2341 gaaaatggag tttatggtct tctaggacca aatggagcag gaaaatctac attaatgaaa
     2401 caaataacaa cgttgataaa acctgatgaa ggtcagatac tttataatgg tgaggatata
     2461 tttaaaatgg atgctgatta tagaaatatt ttaggatatt tgccacaaga atttggagtt
     2521 tataaaaatt ttacagcaaa agaattttta cagtatattg gagctttaaa aggtttaagg
     2581 ggaaaatatt taaatagtaa gataggagaa ttattagatt tagtaggtct ttatgatgtg
     2641 agaaataagt ctataggaaa attttcagga ggtatgaaga ggagagctgg gatagcacaa
     2701 gttttactaa acgaccctaa gataatagtc cttgatgagc caacagcagg tcttgaccca
     2761 caagaaagag ctagatttag gaatttaata gcaaaaatgt ctagagataa gataataata
     2821 ctttctactc atataatttc cgatatagaa tcagttgcaa aggaaacaat aatgattaaa
     2881 gatggcaaac tcctaatgaa aggaacacac aaagacatat taagtcacat ggaaaataaa
     2941 gtatataatg ttcaagttaa aaatgaatgt gaagttgatg atatacaatc taaatataag
     3001 gttgtaagta ttcaaagtga tattaattca atagtactta gaattgtttc agaggatatt
     3061 ccaagcgagg ataatataca accagttcaa gcaaaattcg aagatgtata catgttttat
     3121 tttgatttag aagatgcaag ggaggtgtag tagaaatgat gtatttagtt aaagctgaaa
     3181 tgataaaaat attaaaatca aagagtgttt ggataatttg gctgttttta atattttttg
     3241 gattttttct gattagaaag tttgatgttt tagatactta tgcagatata ttttacaaga
     3301 tagaaggctc aatacctcta ataggtttga taatgtttat aataacttct ggaaattaca
     3361 ttaaagaata tgattctaat atgacaggtt taataaatac aactaaaaag ggaaaaaaat
     3421 cagtagtatt atcaaaacta gtagctaatg gattaatttt atctattatt aatatttctt
     3481 ttatattgtt agtaggaatt agaggtttta gtttttttga ttttaaaaat ttaaatgaat
     3541 cgattcataa cttatggtac tttggaaata gtaatttaaa tcttacagta atacaaatgt
     3601 atataattgt tatattgact actatttttg cttcttttat atttgcacaa ataggcttaa
     3661 cactttcatc tatatttaaa tcggctgtaa ttccatttat actaggtgga ttgataatgg
     3721 caattccata ctttagtgta ggttttatac cagataaagc tataaaattt atgtcagtga
     3781 ctccaaattg gattatgatg agccaacaaa tggttaagta taatgtacca agtattttga
     3841 tagttttttc tatagtaatc tcaattatat taatgatagt gttaactaaa ataacatatg
     3901 aaaattttac atcaagcaaa aggttttaac taaaaataat agtaatttta tttatatttt
     3961 agtttaatta aaaataagat aaatgaattt tagataaaat atatgctaat atatatgtaa
     4021 attattacag gaggttgttt atggaaaatc gtgttttaat aatagatgat gaagtagaaa
     4081 tattgaagct tttagaaact gtcctaaaaa aagaaggcct taataatata tatactgcaa
     4141 aaactaaaaa agaaggatta gaattattta aaagtattaa tcctgatttg attgtattgg
     4201 atattatgtt accagatggc gaaggttatg atatttgtaa agaaataaga aaaacctcca
     4261 attctccaat aatattttta tcagctaaaa ccgaagaatt agataagtta cttggactag
     4321 cgattggagg agatgattat gttacaaaac cttttagccc taaggaagtg gcttttagag
     4381 ttaaagcgca tttgagaaga ttaagttatt ttagtgatgc tcaaaacgaa agtaaaaatc
     4441 taaataataa tgaagaaaaa attatatcat ttggcccata tatattaaat gaaagtaggg
     4501 cagaacttat aaaagatgga aaatctatag gactgtttgc aaaggaacta aaaatcctat
     4561 ctttatttgc acataatcaa aatcaaatta taagtaaaga aaaactatgg gataaagtgt
     4621 ggggagaaga ttatgttgga tttgacaata ctataatggt tcatataaga aaaattaggg
     4681 aaaaattaga ggataatcct tcaaaacctg aatatattct gacaataaaa ggtcttggat
     4741 ataaattagc agtaaaggaa gattagaata tgaagtggaa gattactaga aattttatat
     4801 ttacaattgt gtttgtagct atatcagtag taattataaa tatcatatct atattgtatg
     4861 tgatttcaac taatagcttt tttaaagttg tagattctgg aaataatcct gaagaatttg
     4921 cacgttcttt tgaaaaagat ttatatgaaa aagatggaga atttaaacta tctaagattg
     4981 gagctgaaaa attagaaaaa tcaaattcct ggatacaggt tttaaatgat ttaggagagg
     5041 aagtttatgg agtaaatgtg ccaaaggata ctccaaaaaa atatacaccc tttcaaatgg
     5101 tgaataatta caagtatata gaaacaaagt atgttaattt tgtactagaa aaatatttaa
     5161 acaaaaaaca tttaaacata atagtaggta tacctagtag agatatatca agaataattt
     5221 taacctattc tcaaaataac attaaaaaaa ctttaaataa agttatcatt ataacactag
     5281 taattgatag cgtggtagct ttaggtgtag gatatttatt tagtagaaaa cttacaaaac
     5341 caatttcaag cgtactatgg agtattgaga ctatggctaa tggaaattat agtttatatc
     5401 ttaaggacag aggtatatat gaagaagttt ttaaaaatat aaatatgtta gctgatactt
     5461 taagagtaaa tgaagttgag agaaaagaaa atgaagagtt gagagaggaa tggctggcaa
     5521 atataacaca tgatataaag acacctctag catctataca agggtatgca gagataatta
     5581 atgacaaaga ttatgagttt gaagaagatg aaatacaaga gtatacagaa attatatata
     5641 ataagtctaa gtatataaaa gatttagttg atgatttaaa tttatctact cgattaaaaa
     5701 ataatactat agttctggac aagaaaaaaa taaatttagt cagtttagtc agagatataa
     5761 taattgatat tttaaatgat aatagatata aaaataggaa tattgaattt gaaagtaatg
     5821 aagatttaat tgaagtatat gtagattcaa tcttattcag aagggctata accaatctta
     5881 tatttaattc aatagttcat aatagcgaag gaactttgat aagtgtagaa attgtaaaaa
     5941 aagataatat agaaattata ataaaagata atggaattgg aataagcaaa agtgatttga
     6001 aacatatttt taaaaaatat tatagaggta ctaatacagg tgagatgcat aaaggttcag
     6061 gtcttggtat ggctatttct aaagaaataa tagagattca taaaggtaaa atttatgtaa
     6121 gtagtgaaat tggaatagga acaaaaatta ttatagagat aaaacaaaat taggagatat
     6181 gatatggatt tttttaaaaa aggtataagt gggtttacta ttaaaatttt agcattaata
     6241 tttatgacat ttgaccatat agcagcattt atgccacaaa caatgcaaat accaatttgg
     6301 tttcattggg taggcagaat atcagcacca ctgtttatat tcatggctgt tgaaggattt
     6361 tatcacacta gtaacagaaa aaaatatata agtagattgt atatttggtc tgttattatg
     6421 gctataggaa atcaaataat taataatgta ttttcacatc cagaaggagc tataattata
     6481 aataacattt tttcaacatt atttttaata gcgatttatt tacaagcaat agagttcata
     6541 aagaaattta gaaaagagaa agaaattaaa tattttatta taggattatt gatgataatc
     6601 attcctataa ttttaggaat atttacagta gctttacttt ttaaagtcac taacagagtt
     6661 attgctttat ttatgatttt ggtaccagta ccatttttag tagaaggtgg accaatttgg
     6721 attatacttg ggattatttt ttatttgtgt agaggtaaaa agttcagttt gagtatttgt
     6781 tatgttttaa tgtgtatatt tatatttaca acaatgtcaa atggagatta tagtttaaaa
     6841 aattctattt tacaaaatta ccaatggatg atgattgctt ctctaccttt aatgttactt
     6901 tacaatggag aaaaagggaa gagtatgaag tatttatttt atttatacta tccaatccat
     6961 gtatatatat tgtatatctt aggaatatac ttaataaatg gtttttgatt gtaagtgata
     7021 tttttaatac aaaatgaaca gtatgaggat tgttttaggt ctattaaaat tgttatttag
     7081 atgagatttg tagaatattt aaatttatga gcagaaattc agttattgta atttcaataa
     7141 cattagaata taatattata atttatttag aaatatatga tgttatacat ggtttggtta
     7201 ttaatgtaat aataaaacag aggtttgata taaaattcaa tacctctgtt ttagtgcttt
     7261 ttatcctaca tttatgctta ttttatttat aaattagtgt tttgttttaa ttaatattag
     7321 tacatttttt atttatttta taaaattagt cataattgtt tcttcatatt gtggttcttt
     7381 aattccagct tttttacctg ctgatataca cttaagaagc catgccatgt tttctcctag
     7441 agttcgcata gtctgtaatc cttcttcatc cttcataaca tcctctggag taaagccatg
     7501 tacctggttc caatactgtg atgatacaat aggcatattg ctgattgaaa aatatttatt
     7561 tagttggtca aatgttgcac ttgcaccacc acgtctacat gacacaactg aagcacctaa
     7621 ttttgtagcc atgcgacttc cagctatgaa aaatagacgg tcaagaaaag aagttaattg
     7681 accagttgca ctagaaaagt aaacaggaga accaataata ataccatcaa tattatccag
     7741 atcttcaatc aatttattaa ctttatcatt tcgaatacat tttccagttt caaaacagga
     7801 tgcgcaacta atacatcctg ggattgtttc tttacctaaa tacagtattt ctgttttaat
     7861 atcgtgttta ctcaatactt tagtaatctc atttagtgct gtataagtac atccatattg
     7921 gtttgggcta ccgtttatca ataaaacttt cattataaat ttcctccaat gaattttatt
     7981 taatagattt tctaagagat attatttttt ataaaatagg attttcatga atattatcaa
     8041 tttatataga aatgtctaat cttagaatcc tatttaaaat aacttttttt ttagtaaata
     8101 cacctcctat tataaaattg actttcaatt attcttacac tataatttta ttataaatta
     8161 ttctaaaaac aagtacgcac atttttgttc aatactaacc aaaaggagag tgtaatatat
     8221 gagtatagac tgtatatcaa atacagattt aagtgaaaca ggatttagtt atacactttc
     8281 actgattagt ggtaaatata agatgataat tctttactgt cttgtagaat ttgaagttgt
     8341 aagatataat gcattaaaac gttatataaa tactatctcc tataaaactt tgagtttatc
     8401 attaaaagaa ttagaagcag ataacttgat tattcgtact gaatatcctc aaatacctcc
     8461 taaggtagaa tatagtctat cagaaagggg aaaatctctg attccaattt tagatgcaat
     8521 gtgtgagtgg ggagagagga atagacctta attttgtatc tgtagatacc ataaaaagac
     8581 tgtgagtaaa attgtttgaa taatctactc acagtctata tttaaattaa tagttagtat
     8641 ttttaattta taaaaatact aaaaaattta ttatgaaatt tcaccaccaa tttcttttac
     8701 atcttcttta tatataatac aagcttctat agctaattct aagcctttta ctatttcttc
     8761 taaagacatg aatggtgtat tttttttatc tattacttgt gaagttgtat atgggatgtg
     8821 tataaatcca ccccttatat taggatattt cttgtctatc atataaagaa ttccatacat
     8881 cacatgattg caaacaaatg ttcctgcagt gtttgaaaca ctagctggaa ttttattttc
     8941 attcatatgt tttaccatag cttttacagg caattttgaa aaatatgcag gttcaccatc
     9001 ttcttttata atagtatcta taacctggtt accctcatta tcttttattc taaaatcatc
     9061 tatatttata gcaactctct caggtgtaat gtcaaatcta cctccagctt gtccaactga
     9121 tattactata tctggattgt gctcttgaat agctttatct atagcttcaa cagattttgt
     9181 cataactgtt ggtatagtta tctttatgat ttctgcaccg tttatattat tattgaccct
     9241 ttcaactgct tcttgagctg ggtttattgg ttctccacca aatgggtcaa atcctgttaa
     9301 caaaattttc attattttac ctcctaatta tattaaaatc ctaaaaagta cattactaat
     9361 atgtgggcta taattaatac caaagcaact ggagcttgat attttataac aatataatca
     9421 tttttacatt ctagcaaggc tgctggaact atattaaaat tagcagccat tggagtaagt
     9481 agagttccac aatatcctgc tgttaatgct aatgctccaa ttatagcagg atttccacct
     9541 tgagacaata caaaaggaat accaattcct gccgttatga ctgaaaatgc ggcaaatgca
     9601 tttcccatta tcatggtaaa tataaccata cctaatacat aagctataac accaaataat
     9661 ctgctatcag cagggactac accagatatc atggttgaaa taacttcacc gactccggct
     9721 tgagtaaata aagctcccaa tgcaactaaa agttgtggta acatactagc aggtcccatt
     9781 tgttgaagta atcttgagcc atcttgaact gatgtagcag gttttgcctt agttattata
     9841 agggctgata ttaatgcaat tattgaaccc acacctattg caaattgaga agaattatca
     9901 aaattagaaa ggctcatagc tactactact gcagcaaatg ctaatattaa tgatggtata
     9961 aatattttat ttttaagttt gtctgcttgt tcttttccaa aattttcatc tggttcttca
    10021 tactttgcaa atacaacttg tttagaaagt gttaataaac tagataatat taatattatt
    10081 cctattatat tggctggaat caatctacca aatatgaatg gcagtgatat aagtatccaa
    10141 aataatgatg taccatattt cttattgtta tttatggtag taaaagactt atatgctata
    10201 acaatcatta aaaatcccat tagtgcgtaa aagatttcta atgatatgtt tataaaattt
    10261 tgcattttta ttcctcctaa ataaaactat tttttatgac ttttgtttaa ttttttatca
    10321 tataaggtaa actggattgt agccactaca agtgctatta ctgccataat agtactagcc
    10381 tttacgatat taagtccagt tacattgtat ccaaattgag tcagagtaga tgctataagt
    10441 aatactccac tactacctgc aaataagttt tgaccataaa aatttccata gttttctgat
    10501 gcagcagata atgcctttat ggcttcttca tccgattctt ttagttcatc actattactt
    10561 aaaccagcag cttgtgccat aggattaaca agtggtctta caaattgagg atgtccactc
    10621 attcttattg atagagcacc agctatttgt ctagctatca tgtatatagt aagtactttt
    10681 cctgttgtaa gtctttttaa tttttctatt aaagaaacag ctctttgttt taaaccatat
    10741 ctttctaata caccgattac tggcaatgtt aatatgaata atgatacacc tctgtttgag
    10801 acaaaagaat ctcctaaaat tgtaaaaatt tgattaaaat ctaatcctgc aacaattcct
    10861 gttgccactc ctgctaataa tacagtaaat aatgtgtcaa ttttaagtat aaatccaatt
    10921 acaactatca atataccgat tagttttatc ataaattgtt ctccttccat ttctatatgt
    10981 tttaatttaa atttatttaa tggataaagt taaactcttt ttatagtatt tgcgttaata
    11041 ggactattat tataaaattt aataaaaaat agggaagatg ttttaaagta tcaaagtaaa
    11101 taatggttgt gtataggaat ttgaataaca aagattatat tttaagctaa tatgaattga
    11161 ttttgtaagt ctatagttta agtatatgtc aatatttgaa ataggtttta aattagatat
    11221 tatattataa aaagagaatg tgccaaacat agagacaagt gtatttttca cttttgaaat
    11281 gataaaaata tatttgattt catacaaaaa gagagtgtct aatgaatttt ttagttcatt
    11341 agacactctc tttttgttat gattaataaa acatatttgt tatgattaat aaagcatata
    11401 ttaagtgcta gtttcattag atttcactaa gaaaaataaa attttgataa agtttaaaga
    11461 ctattttgag caatattact tataaataaa agaatttatc atagaaataa ttagttttat
    11521 tttagctatt aagatttatt aaaaaattat ctatattatc ctgtgatatt ctaaaactgt
    11581 cataattacc ttttgaaaca gccttcataa taagtttatc taaaaatttc attttttcta
    11641 atcgagcttc actaccaaaa attccaatag catttgcact ttctaataag gatgatggaa
    11701 aatttgattt taaatattta tctgcttgtt cagaaaagcc acagcaaagg aaaatgccaa
    11761 cttgtttttt gtttaataac tctacattat cattacatag cacttgtatt ttttttgaaa
    11821 ctgaaccaac atatatagaa cttccaataa taattttatc aaattttgat aactcaattt
    11881 tattattatt attaatatca acaatagtga cattatctga tagtttattt tttagtatat
    11941 ttgcacagtc ttttgtacaa ccatatttac ttgaatatat aataatagta ttcataatta
    12001 ttctcccttt cttaaagcat tttctacagc ttttcgtata gtattactgc tataagttgc
    12061 agaagttata gcatccacat ctaaagactg ttgtttaata atacttgttg taattttttc
    12121 agcaggttta cctaaaagat ggtcgtgttt taagatatta atattttgga ttttaccatt
    12181 attaacttca acagacacag tagcttttac caagttatta tcagcacttc cagtataaat
    12241 gccattttta acattattaa tatctatatc ttttactacc aagttttctg gctttaagtt
    12301 gcttacaatt acaataataa tgcatataaa tacaaagaat agagcaatag atatttttac
    12361 atattttttc tttttattag tagccttgtt gcgaattttc attgtaaagt cctcccacta
    12421 aaatatctaa gaaattttct ataagttgag aaaaattatg taaagaatat agtttatttt
    12481 caacaacaca taaaacgaat cctaacaatg cacttataag catccatgag cttttatcta
    12541 tatttttttt gaatattcct tgttcaattc caatggaaag gaatttatca agcattttta
    12601 taccattatt gctaggcgta ttgtttgtat tttcagatgc aaatataata ttcattctac
    12661 tatacatgat agattttgcc atctctggtt cactagaaag agtttttata aagctagtca
    12721 taatttctat gatttgttta tctattggta gtgatgagca attattcata atgtcaatgg
    12781 cattactttc tactgtgtta taaagtttat tggtcatatc gctgataatc tctgctttgt
    12841 ctttgtagta aagataaatt gtagttggag aatattctat ttttgtagca atttttctta
    12901 ttgataaatt ttcatatcca tgttggttta taatttcgat gctagcatcc ataatctttt
    12961 tcttcatttc ttctttttca tttttacgtc tttctttgat actcatataa tcacctcttt
    13021 gcttaacggt gttaagttaa tttacactgt tattgtataa tttaatttta atactgtcaa
    13081 taaattatta aataatataa gttaatatat ttttatggat aataaattaa tgttattaat
    13141 tgtaatattt aagatattat tgtataataa taatcggttt aaatgcgcca catcttatta
    13201 aagttggttc ataattctaa ataaacttta ataagataca ttaaaatata ataaattctt
    13261 ttatgctata aaatttattt tatagctatt aaatcagatg tttaaaaatt ttttaacagt
    13321 ctatagtttt taagagtcta tagtaagtag cctaaaaata tgattttaca tctgtacttg
    13381 ttcgagaact ccaagtataa aaaactaagg aattatatag tggcagtata taattaatac
    13441 ccttagacac acctttttga aagtggaaaa aagatgtgta gttctcctcg accacaaatc
    13501 aaggaggata aaaaatggaa aagagaaaag taataattga ttgtgaccca ggaattgatg
    13561 attctttggc aattcttctg gctttaaact caccagagct agaagtaatt ggaattacca
    13621 catgttgtgg aaatgttcca gcaaatatag gtgcagaaaa tgcactaaaa acacttcaaa
    13681 tgtgttcttc actaaatatt ccagtatata taggagaaga agcaccacta aaaagaaaac
    13741 ttgtaacagc tcaagataca catggagaag atggtattgg agaaaacttt tatcaaaagg
    13801 ttgtaggagc taaagcaaaa aatggagcag tggattttat aataaatact ttatataatc
    13861 atgaaaaagt atcaataata gcacttgcac cacttacaaa tatagctaaa gcacttatta
    13921 aagataagaa agcatttgaa aatctcgatg agtttgtatc tatgggagga gcatttagga
    13981 ttcatggaaa ttgctctcca gtagcagagt ttaattattg ggtagaccca catggagcag
    14041 attatgttta caagaattta tctaaaaaaa tccacatggt aggtttagat gtaactagaa
    14101 aaattgtact tactcctaat attattgagt ttataaatag acttgataag aagatggcaa
    14161 agtatataac tgaaataact agattttata ttgatttcca ttgggaacag gagggaataa
    14221 ttggctgtgt gataaatgac cctctagcag tagcgtactt tatagataga agtatatgta
    14281 aaggatttga atcatatgta gaagttgtag aagatggaat agctatgggt cagtctatag
    14341 tggattcttt caatttctat aaaaaaaatc ctaatgcaat tgttctaaat gaagttgatg
    14401 agaagaaatt tatgtacatg tttttaaaga ggctttttaa aggttatgaa gacattatag
    14461 actctgtgga aggagtgata tagtgtgaaa agaaaaatat gtgtaaaaac tattacactt
    14521 atagcttttg gtatttctat aaatataata ggcgctttta tagctatggg attaagatta
    14581 cctgtatact tagattcaat aggaactata atgatagctt cacttttagg acctaaatac
    14641 gctgttgtta caggtgtgtt tggaagttta ataagtggaa ttacgtttga tgtgtattct
    14701 ctctattttg caccagttca aatatcaacc ggattacttg ctggaatagc atttaaaaag
    14761 aattttttaa ggggtttaaa aactccacta ggagtacttt tatttgccat tccaacatct
    14821 ataataagtt caataatttc agcattttta tttggaggta tgacctcttc aggttcatca
    14881 tatatagtgc aaatcttgaa agtgttagga ctgggggatg tttttagtgt gtttgtaacc
    14941 caagtattta ctgattatgg agataagctg ttagcagttg ttttagtgaa tttaggatta
    15001 aatgcagttc caaaaacttt aaaggtatca ttgactggag gaaaatagat agaaaatgga
    15061 tagatatagt gagataacaa ataaaaatca aagagaaatt gttctgctta aaggatttcc
    15121 ttgtatatgg ggaaagtgtt ctttttgtga ttatatagat gataattcta atttagaaga
    15181 agagatgaat aaattaaatc taaaggtttt aaaaaacgta actgggaaat atggagttct
    15241 tgaagtcata aattctggaa gctgttttga attacctaag gatactttag aaaaaataaa
    15301 gtgtattata aaagagaaaa atataaaaaa attattttta gaaagtcatt ggtcttacaa
    15361 aaataggctt aaggaaatga gagagtattt tgaaattcct gtggtattta aaattggagt
    15421 tgaaaccttt gacaatgatt ttagaaataa tattttaaat aaaaatgcaa attttaagac
    15481 tccacaagat gtaaaagaat attttgattc accttgtatt atggttggaa taaaaggaca
    15541 aacaaaagag atgatagata aagatataga gattatccta aatacttttg aaaaagcgac
    15601 agtgaatgtt tttataaata attcttctag tataaaaaga gatgaagagt tagtgaaatg
    15661 gtttgttagt aagtataaat tcttggatga aaatccaaat atagaagttt tatacaataa
    15721 tacagatttt ggtgtaggag attagcatta atttagttta aatttaaaat tgaaaagttt
    15781 cttatgtttt tgaaaaagct tctaatagtt agaagctttt tttgtagagt tttatgttac
    15841 aataagaaaa aaggaattaa ggagcaatta aatgatgttt gatagggtaa aatggaataa
    15901 attggattat aatgaacttt tagaatatat gatgtctata caagatataa aatatagaga
    15961 ttttaataaa aagcttatac ctggaacaga aaatataata ggtattagag ttcctaattt
    16021 aagaaagtta tcaaaagaaa taagccaagg gaattggaag gaatttttag aagtagcaga
    16081 ggatacatac tatgaagaag ttaggctaca aggtatggta attggcaata taaatagtaa
    16141 ttttgaagaa actttatact atgtaaaaag atttatacct aaaatcgata attggtcagt
    16201 ttgcgatgga ttttgttctg atttaaagtc tatgaaaaaa tataaagaaa atatgtatga
    16261 tatacttaaa aaatatgttt attcaaagaa tccatgggaa attagatttg cattagttat
    16321 gtttttaata tattatgttg atgataaaca tattaaagaa atatttgaat actgtaataa
    16381 tatacaaagt gaagaatatt atgttaaaat gggtatggca tggttgctat caatttgttt
    16441 tattaaatgt gaagaggaaa cttttctcta tataaaaaat aataatttag atgatttcac
    16501 ttataataaa atgctacaaa aaataattga atcaaataga gtagatttag aaaagaagaa
    16561 tataataaga agtatgaaaa gaaagacaag aagatgttga cggaggaata ttaatgaata
    16621 acaatgaaga cagaattgac aaatttttag aacttcaaag caaaggattt aaatttgcag
    16681 agatagctca aaatctaaag attagtcaaa gtactttaag aactttttta aataaaaaag
    16741 gatataagtc agaaaaaggt aaatacatat ttaaagacga attaaattat aataaaagtt
    16801 tggataatag aaacttggaa gaagagaaag ctaaggagta tgaaaaaagt aaaaagaata
    16861 ctataagtaa tagaaaaaaa gtaagcacca aaaaaaagat ttctaatgaa aaggctttaa
    16921 gtaaaagtgt tgaacaaatt gagtttaatg atacaggttt aaaaaatgca aagccaaaac
    16981 aaaatattaa gcctaagaaa gatagaaaaa taaatataac tcaagaagat ttagataagc
    17041 tttgtgaagt atatgattgg tatttagaag taaaagatta caatataaag cctaaaaaag
    17101 tatctaataa aaaagatgta catatagatg atgaacacat agatgaatta aaaacaacaa
    17161 gaatacgtat agataaagaa atatggaatg attttgaaag attatgtagt aattcgaatt
    17221 ttgaaaaaca tcaaatactt acacaggcat tgaaagaatt tatgaaaaag tataaaaatc
    17281 tactataaaa tactactata tgaggtgctt aatatgaata aaatagtaaa aaaatcaatt
    17341 tctctaatga ttatattaac aatatttatt tttatgttga cagcatgtga aaaagatgaa
    17401 cagcctgatt cagttgatat tcaaacagaa gataaaaatg agataaaaat agatgaggga
    17461 aatgcaaaag tattgaatat aggaaaatca gaaattataa atatagatga aggtgataaa
    17521 gtagatacat caacaaaggt agaaaataat tcaacattta atatatccaa tgttgaattg
    17581 atatataatg aatatgatga gaataaaaaa attatatctt cggatagtaa ggctttgttg
    17641 gacatgactt taatgcctgg gaaagttgct tacatagaat gtgggcataa aacttttgta
    17701 aagggtgtag aagtttatgc atatgaatat gaggcagagg gtaagattgt gtatgttaat
    17761 ttaaaagaaa atactattaa tataagaaat aataatatta agctagaaaa tagtagtcag
    17821 tatgaggtgc tatctacatc agaacttaaa aaagtaaatg aatcaaatga gggaattacg
    17881 tatcaagtaa aagtaaaaaa ttcatcatca aaagatttag gaaatattat tcttaagaca
    17941 gcagaagtta atgaaaatgg agaatattta acagtaaatc gtgtgccatc atataagatt
    18001 ttaaagcctt cagaagagac agatatagat atattgtgtt caacaaaggc aaaaagtgtt
    18061 gaaatagtgg gatatacgta tgatgatata aaagaaaaag ccaatgtgga tattgactta
    18121 aaatctcata aagttaagat agacaaataa aggtagggga tacttatgaa ggttttaata
    18181 gttgaagata ataagatact tttagaaagt gtagtagaag aacttagcaa gcattttgaa
    18241 actgaaaagt gtgaagatgg agaagaagct ttatacttag taaatcaaaa tatatatgat
    18301 ttagtcattt tagacttaat gcttcctaat ataaatggat ttgatatcct aaagaaaatg
    18361 agagttaata atatagatac acctgtactt atattgacag caaaagaaac attagatgac
    18421 aaagtagaag catttactat aggagctaat gattatttga caaaaccttt ttatatggaa
    18481 gagttagtag ctagggtata cgctatatta aggactaatg gaaagataaa agaaagaaac
    18541 ggattagaat ttaaatcttt atatttagat acactagaaa aaagagtgta tattgaaaaa
    18601 gaagaaataa aacttcaaaa caaacagttt aacttgcttg agtattttgt attaaataaa
    18661 ggttcaattt tattaaaaga gcagatttat gatagaatat ggggaattga ttcagatgcg
    18721 actattgaaa tagtggaagt atatgtaagt aatctaagaa aaaaactaag taaatatgga
    18781 tatgataaat atattaaaac taaacgtaag gtaggatata tattcgatga taaataagaa
    18841 tgtatttacc agtactaaaa atcatttaat aaaaatgtat attattgtag taggtagttt
    18901 tttaataatc ttttctatat ttatatactc atattttagg ggacttacat atagtggaat
    18961 tgatagtgag ataaatgatg aacttgaata tatagtctct caatttaaaa gaacttcatt
    19021 tttgaaccca ataagactga aagatcctaa agatatggtg tatgtgtatg aagatggaag
    19081 gataagttac tatactcaaa atgaatattt tgatgagtta cttccagata gaagactaga
    19141 taaaaaaaat tcgtttttta aatacacaga aaatggatat acttttagag aattaaatgt
    19201 agatgtaggt agataccaaa tacagataat tagaaatata gattctgaga tgaattcttt
    19261 aagacagctt acatctgttc ttataattgg aatattaatt tctgtaataa ttacttactt
    19321 tgtggctgta tatctcacta gaaaagcttt aattccaata gaaacagcat ggaaaaatca
    19381 agctaagttt atacaagatg cttctcatga attaagaaca ccaataacaa tagtttcatc
    19441 aaaattagaa agtatgttaa aatccccaga aagtacagta aatgatgaag ttgaaacaat
    19501 agctactgca atgaaggaaa ctagaagact taaaaaaatg ataactgatt tattgtctct
    19561 tactaaagag gactctattg ttaaagttaa tttagaagaa atagatttag aaaagttatt
    19621 ggaagaaata tcagaagact atattgacat tgctgagttt caggaaaaaa gattcgtgtt
    19681 taattctaaa cttaagaata aagttataat aacagataaa aataaattaa gacagcttat
    19741 attaattttc attgataatg cctttaagta tacaaaattg ggtgatgaga tagcattaga
    19801 attgaaagaa gatatagagg atgaggtaac tttacttata agtgacactg gtataggcat
    19861 aaaaaaagaa gagatacctc ttatatttga tagatttttt agaagtgaaa atgtaagaaa
    19921 taaagattta gaaggttctg gaataggact ttcaatagct cgcatgatta gtttaaactt
    19981 atctattgat ataaatgtaa cttctgatgt agatattgga actacatttg aattgtcaat
    20041 acccaaaaag ttaaaataat aaaaatataa aaaattgttt ttagaaatga agaatgggta
    20101 tactaattag tataaaatat aaaaagacct tataaaagta aatttatatt tataaatttc
    20161 atagtataat tatataggga taataaagtt taataggagg atttaaaaat ggcaaaagtt
    20221 ataaatacat ctgaatttag aagtagtgtt gaaggtagta agggagtagt tgtagttgat
    20281 ttctttgcaa cttggtgtgg accttgtaac atgttgggac ctgtatttgc agaattaggt
    20341 gaagagatga aggataaagc tagatttgta aaagttgata tagatgagag cttagaaata
    20401 gctcaacaat tcaatgtatc tacagtacca actatgatta tatttaaaga tggaaaacca
    20461 gtagaaactt taattggatt catgccaaag aacaaaattg aaatgcaagt aaaatcatat
    20521 ttataaaaaa tgggtgagta ttatgagata tgatatagct ataatcggca gtggtccagc
    20581 tggattgtct gctgctataa atgcaaaaat aagaaataaa acaattatca tgtttggtaa
    20641 tgataattta agtaataagc ttgttaaagc tccttcaata gataattatt taggttttta
    20701 tgatataagt ggtgatgaat taaaagacaa gtttaaatca catatagata gtatggatat
    20761 ttcaattgaa aataaaagaa taaataatat ttatgctatg ggagaatact ttacaataat
    20821 gtctggaaat gatatgtatg aagctactac tgtaatcctt gcaactgggg tagagtatac
    20881 tagaccaatc aagggtgaag aagaatttct aggaagaggt gtgggctact gtgctacatg
    20941 tgatgcacca ctttatagaa ataaaaaggt agcagttata ggatataatg aagaatctaa
    21001 agaagaagca aattttttaa gtgaactcac ttcaaagact tattttatac ctatgtacaa
    21061 aaaagataat cttatgagaa gttcagataa cctagatgat tctattgaag ttatacatga
    21121 cagaccagta cagatagatg gagataaact tgtaaacaaa gtatctttta aagaaaacca
    21181 cattgaagtt gatggagtat tcgttataaa agatagtaca gctccaagtg cattggttcc
    21241 tggaatagag attgatggca ttcatattaa agttgacaat aatatgaaga ctagtataga
    21301 tggatgcttt gctgctggag attgtgttgg aaaaccatat tcttatataa aagcagctgg
    21361 tcaagggcaa atagcagctt taaatgctgt ttactattta gataaattaa agagggctta
    21421 atatttatat tgattaactt tttaaagata tttataaaaa actgtcttga aataatttta
    21481 ttatttcaag acagtttttt atttttaagt tattaataaa agtattttaa aatctatatg
    21541 taattacttt tattaataaa actcaaagtt atattagtaa aaattggaat tatttataat
    21601 atactccaaa atttaaaaat aatggttgaa aaatctaatt aagagtacta taatttaatt
    21661 aagtaattac ttaatcaaaa cgaggtactt atggaaaact tggttaaaat atttaaagca
    21721 ttgagtgatg aaactagact taacatactt atattagtat ccaagagaaa tatatgtcaa
    21781 aaggggattt ctaaatatct agggatttca gattctgctg tttcacaaca tataaaaata
    21841 ttaaaagatg ttgggataat aacaggatat aaagaaggat actatgtttt atatcatatt
    21901 aataaagaat catttaatat atgtgtagat tttataaatt ctatgttaag taattctagt
    21961 gaaagcttta tagatgtatt tgatgtaaat acaatacatc taggatgcag taaagaatgt
    22021 aaatctataa aaaaatgttg taagagaagg gagaaataat aatgaaagta tgtataccag
    22081 tagaggagaa taaaggattg gatagtaaac catatggaca ttttggttct gctccaattt
    22141 ttgttgtgtg tgatttagaa agtggagaag ttaaatcttt agataatgga gatttagatc
    22201 atgaacatgg aaaatgtcaa ccattaaaag cattgtcagg gacagctgta gatgcagtag
    22261 ttgttggagg aataggacaa ggagctataa ttaaattaaa tggaatggga ataaaagttt
    22321 acagagcaga aggtgatact ataagtgcta atttagactt acttaaaaat ggaaaattag
    22381 tagaatttcc atcagaccat acttgctcac atgacggatg tggacatcat taaaaaaaaa
    22441 cagctgatga aatatatact tcatcagcta ttttttcttt taataaatac gattttattc
    22501 ttttaaaacc aaaatttaga tttttatatt ttttgtagta tccttaataa tatttcaagt
    22561 ttacaagttt actactttta gatttttttt gtatgttttt ctatatagtc aatatcattt
    22621 aatatgtcat tgagttcttt attaagtgtt tttaaatcat caccatctac attgtagttt
    22681 aaaaatagtt cctctacatt atcaatgata gaattgactt ttctagtaac ttcaacaaat
    22741 gtcttaaagt tactactatt tttagcattt agaaattttt ttaaatcctt tgatataaat
    22801 gatactattt catattcatc attaaataga cctctaccaa taggatatgg ttcaattctc
    22861 tgtaaaaaat attggtcttt agcattaata ttataagtat aagatatttc ccaaccgtct
    22921 cctaaatcaa acttgataaa atataatgta ctacttactt ttgatattct tgtggcacca
    22981 agctgtctta aaagaacttc aatcgaatca ccttgaacag tattaatatt catatataca
    23041 catccccttt agtattatta tactatattt tttaaaattt tataattaaa taaacagtag
    23101 atataataat agatataatc ataagtggaa aacctatttt gaaatactcc ttaaaagaga
    23161 taggatgacc gtgtttatta cctatacctg ctagtacaac attagcagat gctcctatta
    23221 gtgttccatt tcctccaaga caagctccaa gagatgttgc ccaccatagt ggcattacat
    23281 caataccttc agcttgcata gtcaaaataa gaggaattag tgttgcaaca aatggaatat
    23341 tatctaagaa agaagagact atagctgaca accataatat caacaacatt gtaaatacaa
    23401 gatgtccttc tgtaagtcca atcaatgctt gagccaattt gtttattatc ccaacttcta
    23461 ctaatccacc aactactaca aataatccca taaagaataa aattgtagac cattcaatac
    23521 ctgccataat ttcatctaca tcttgttttc ctattaaaag cattatagat gctcctgtca
    23581 gtgctacaac agaagaatct atgtggattg tactgtgaaa cataaaacca aaaagtatga
    23641 atgctattaa tataaggctt tttataagta gaggtttatc tttaacagat tttttttcat
    23701 ctagttttaa tattgcattt ttagctcttt cattcacaac aagttctttt ccataaataa
    23761 atctaaagca tattatagtt atagcaagta ttactataac agcaggacct aaatttatta
    23821 caaaatccat aaaactaaga ttggcagcac taccaatcat aatatttgga gggtctccta
    23881 taagagtagc tgttcctcct atatttgaag ctaaaatctg tgttattaaa aatggaactg
    23941 gattaagtcc taatatttgt gtaataacta tagtcattgg tccaataagt aagacggttg
    24001 taacattatc taatacagct gataaaatag ctgttataat agcaaagcaa atcattatct
    24061 tccatgggtc acccttagct ttctttgcag tccagatagc tatatattca aataaccccg
    24121 agtttttgac aacagctaca aatagcatca taccaactaa tacaccaata gtattgaaat
    24181 ctatatggct taatccttca tcaagtgtaa gtatattgaa tattatcatt aatagtgcac
    24241 caccaacagc tgctacggat ctgtttacct tttctgtaat aatagctgcc ataactatta
    24301 aaaatataca tatagctatt atttgttgaa attccataat ataagtacct ccatttgtat
    24361 tattttgtgc ctaatgctag tactatatat agtattactt ttttattgtt ttttcaatgg
    24421 atgtatgaaa gtatattatt taacaaagtt tttgaaaaat tgacagaatt taaagaaaat
    24481 ttttgttatt taaataaatt ttaaaattat tccattgata ataatattaa acgacaaacg
    24541 aaaaaatata cccttta
//
LOCUS       DAMJWU010000046        24263 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMJWU010000046 DAMJWU010000000
VERSION     DAMJWU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.046"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            81..902
                     /locus_tag="PC460_002104"
     CDS             81..902
                     /locus_tag="PC460_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2600988.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            923..2119
                     /locus_tag="PC460_002105"
     CDS             923..2119
                     /locus_tag="PC460_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2600989.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            2286..5684
                     /locus_tag="PC460_002106"
     CDS             2286..5684
                     /locus_tag="PC460_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2600990.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5694..7367
                     /locus_tag="PC460_002107"
     CDS             5694..7367
                     /locus_tag="PC460_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2600991.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7640..7834
                     /locus_tag="PC460_002108"
     CDS             7640..7834
                     /locus_tag="PC460_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2600992.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    8003..8236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8313..9710
                     /gene="asnS"
                     /locus_tag="PC460_002109"
     CDS             8313..9710
                     /gene="asnS"
                     /locus_tag="PC460_002109"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2600993.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9883..11058)
                     /locus_tag="PC460_002110"
     CDS             complement(9883..11058)
                     /locus_tag="PC460_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2600994.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11477..12841
                     /locus_tag="PC460_002111"
     CDS             11477..12841
                     /locus_tag="PC460_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2600995.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12863..13354
                     /locus_tag="PC460_002112"
     CDS             12863..13354
                     /locus_tag="PC460_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2600996.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13626..14297
                     /locus_tag="PC460_002113"
     CDS             13626..14297
                     /locus_tag="PC460_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2600997.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMKRLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14382..15248
                     /locus_tag="PC460_002114"
     CDS             14382..15248
                     /locus_tag="PC460_002114"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2600998.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15281..16783
                     /locus_tag="PC460_002115"
     CDS             15281..16783
                     /locus_tag="PC460_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2600999.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16913..17365
                     /locus_tag="PC460_002116"
     CDS             16913..17365
                     /locus_tag="PC460_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2601000.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17525..18328
                     /locus_tag="PC460_002117"
     CDS             17525..18328
                     /locus_tag="PC460_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601001.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18386..19273
                     /locus_tag="PC460_002118"
     CDS             18386..19273
                     /locus_tag="PC460_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2601002.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKDMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19292..20140
                     /locus_tag="PC460_002119"
     CDS             19292..20140
                     /locus_tag="PC460_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2601003.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20203..20892
                     /locus_tag="PC460_002120"
     CDS             20203..20892
                     /locus_tag="PC460_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2601004.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(21222..22412)
                     /locus_tag="PC460_002121"
     CDS             complement(21222..22412)
                     /locus_tag="PC460_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2601005.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYAWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLDDGIDTAGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSERGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEPKVILTPEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLRDSEIGYYIDTPKTKNGKRKIPLTE
                     RAYQALQRILKNRVKAQPLVVGGYSNFLFLNREGLPKVAGNYVGMVRGLIKKYNKYHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(22490..22693)
                     /locus_tag="PC460_002122"
     CDS             complement(22490..22693)
                     /locus_tag="PC460_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2601006.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(23075..23323)
                     /locus_tag="PC460_002123"
     CDS             complement(23075..23323)
                     /locus_tag="PC460_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601007.1"
                     /translation="MKHTFKKPSYYLLSQAMDGDEKAIEKVLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            complement(23327..23746)
                     /locus_tag="PC460_002124"
     CDS             complement(23327..23746)
                     /locus_tag="PC460_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2601008.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQQGLKRRKEKEISFC
                     ELPEIMVENLAVWDDYETDYTIFNVCGNDIRVYDDELAEALKQLSERNRETLLMYYFL
                     EMNNEEIAKKQNISRSGVFQNRHNSLALMRKLLKEKR"
BASE COUNT         9106 a         2911 c         4359 g         7887 t
ORIGIN      
        1 ttgtattatt atttttatat caaaaatagt aataaagtct atatacattt agaaaatact
       61 gtttaaaagg agtaggattt atgataaaaa aagagatatc taactttaga ggaagtagtg
      121 attatgtagt atcaccagaa ttgatggcat cagttaatgt tgctattgct cttgaaaagc
      181 cacttttgat taagggcgaa cctggaacag gtaaaactat gctagcacaa gcaatttcaa
      241 atgaattaaa aaaagattta gtaatttgga atataaaatc aactacaaaa gcacaagaag
      301 ggctttatgt atatgatact gttcaaagac tttatgatag ccaatttgga ggagaaggtg
      361 tagatgatat tagcaagtac ataaaatatg gtaaactagg agaggccttt agctcaaatc
      421 aacaagtaat tcttttaata gatgaaatag ataaggcgga tttagaattt ccaaatgact
      481 tgctttggga attggataaa atggagtttt acataaatga gacaaaagaa actgtaagag
      541 ctaaacaaag accaatagtt attataactt caaatgcgga aaaagaactt ccagatgctt
      601 ttttaagaag atgcatattt cattatatag aatttcctga tagagacatg atggaagaaa
      661 tagttaaagt acattttgat aaagtagaag aacacctatt ggaacaagta atgactactt
      721 tttattggat tagaagttta aaggacatac aaaaaaagcc tagtacatca gagttaatag
      781 actggataca agcattaact ttgagtggaa tgcctattga aaaaatagaa aaagaagttc
      841 cttttgcagg aatactactt aagaataatg aggatattga atctatgcaa agacacttat
      901 agttaatagg agctggataa ttatgtttat tgaatttttt tatttattga ggtcaagagg
      961 attaaatgta tctttaaatg aatggatgac tttaattgaa gcattggata aaggactgtg
     1021 ctattcaaac ttttcaaatt tttactatct ttgcaggatg attttaataa aaagtgaaag
     1081 tgattttgat aagtttgatg cagtgttttt ggagtatttt aaaggtatag aacaccaaga
     1141 agaaattcca gaagagatta tgaattggct taaaaaacca gatatagatt tagaagagtt
     1201 tgaaagatta gaacaaaatc caaatataaa tcttgataaa ttaagagcta aattggagga
     1261 acgaataaaa gaacaagatt ctcctcataa tggtggtaat tattggatag gcacaggagg
     1321 cacttcagag ttaggacatt caggaaaagg gcaaactggt atacgtatag gtggaaattc
     1381 tacttatggt agagctgttc ttgaagttgc aggagaaaga aaatatagag attttagaga
     1441 tgatgaagtt ttaaatatgc gtcaatttca agttgcacta aagagacttc gtcaattttc
     1501 tactagaata gatgctccaa agacagagct tgatttggat aagactattg aagagacttg
     1561 taataatgca ggatatttaa agttattttt tgagaagcca agaaaaaata cagtgaagct
     1621 tttattgctt atggattcag gtggttcaat gagagggtat agcactttat gtaatacatt
     1681 atttcagtct gtaagtaaat caaatcattt taaagatgta aagatatatt atttccataa
     1741 ctgtatttat gatagattat ttacaacacc tgaatgttgg ttaagtaaat ctattaatac
     1801 tgaatggata ttaaaaaata tagataaaaa ctataaagtt ataattgttg gagatgcttc
     1861 tatgtcgcct tctgagttgc ttcatattgg tggtaactat cgtggtccat ataattacac
     1921 acctggtata gagtggctta aaaggtttaa gagaaagtac agtaaaattg tatggatgaa
     1981 tccagaactt agagatggat gggattcaat taattattgg tatcaaactc aaagaatgat
     2041 acaaagtgaa tttaatatgt ttccattgac tgtaaaaggt cttgagaaag cattgaaaaa
     2101 tttaatggtt tcaagataaa gtaaatataa atgatggtat gatttattca aaattgtacc
     2161 atcattattt atttttttaa tatacctatt ataaattgta taactgaaac ttaaaaaatt
     2221 atatagtcta atacatttta gactcacaca aaacatatac attaataata attttagaag
     2281 tcgaggtgat tataataaat tatgaattaa ttgtaaaata caatggagat atattgaggt
     2341 tagaagaaga attaggtgta tcagtagaga tacttaattc atcatatgca ataataacat
     2401 caagtaatga agaagatgta aatatacttt taacttatcc agaaattgaa tttatagaaa
     2461 agccttttat attacagact caggatgtgc aaagtttttc aagtacagga ataacaggat
     2521 ttaaaaatag aactggttta actggaaaag gaactataat tggtataatt gattcgggaa
     2581 tagattatac tctacctgtg tttagagata gtgatggaag gtctaagata ttatattatt
     2641 gggaccaatc tattcaagga aatccaccag aaggttttag agaaggaaca ttatatacta
     2701 atgaagatat aaataatgca atagatggaa gtatgtatat acctatttca acaactagct
     2761 tacatgggac acatgttgca ggtatttgtg caactattgc aagtgatgcc agaataatag
     2821 tggttagagt tggaaatata caaaccgata ttttttcgag aagtacagag tttatgaggg
     2881 caattaaatt tattttagat agggcattag aattaagaat gcctgttaca ttaaatataa
     2941 gttatggaag taatgaaggt tcacatagag gaacatcttt atttgaacaa tatatagatg
     3001 atatgtgttt attttggaaa aacaatatag ttgtagcagc tggaaataat gcagataaag
     3061 gaggtcataa aaggattagg cttcaaaata atattacaga agaagttgag tttatagttg
     3121 gggaaggaga gcgtatatta aacataaata tatggccaga ttttgttgat gattttagtg
     3181 tacatttggt aaacccatca aataatcaaa cacaagcaat ctctctaact tcaggtgaga
     3241 taaggaatac tttgggtgaa actagaataa ctggttattt ttaccctata gcaccgtatt
     3301 ctctcacaag aagagttact ctacaattaa gttcaaatac acagataact cctgggttat
     3361 ggaaaattgt atttgaacca atagatatag taactggtaa tgtaaatata tatttgccaa
     3421 cttctgaagg tctaaataga aatactagat ttctgattcc tactcaagag cttactgtaa
     3481 cagttcctgg aacagcaagt agagttataa cagtaggtag ttttaattca agaacagata
     3541 tagtatctat attttctggt gaaggagata cacaattagg tgtttttaaa cctgatttat
     3601 tagctccagg tgaagatata gtatcttttt taccaggagg tacaagtgga gcattaactg
     3661 gaactagcat ggctacacct catgttacag gggtttgttc attgtttatg gaatggggaa
     3721 ttgtaaatgg aaatgattta tttttgtatt cacagaaatt aagggcatta cttctaaagg
     3781 gagcaaggag attaagtaat caatcttatc caaataattc atcaggattt ggttttttaa
     3841 atttatcaga tatagatttg tacactctat ctaatataaa tcaagattta gaaacagagg
     3901 atattggata tagaagtatt aataagtcat ttaaagatga ggaaaatagt tataagttta
     3961 tagatggtta taatatgcaa atacataatg acttagagaa tgaaatttat ataagtaaaa
     4021 atgcaagtag acaatcagga atactatcag gaatagatat agtccatact cctgaatttg
     4081 aagaagaact agctgggtta ggtatgagtc aaaggttttt taagataagt gattcattag
     4141 gtgtactatc tataaataat acagactata atagcataca acgagttttg caacttcctt
     4201 caatcataag aactgtatcg actacaaaga tgacattact tggagaaatt aatcgaggta
     4261 cttttggagg tgtagttgca acagaagaaa tgggcgtaaa tttctttaaa aataatccta
     4321 atattaatat aacaggaaga gggactctaa tatcaattgc agatacagga atagactatc
     4381 tacacccaga ttttatttat ccagatggca cttcaaaaat agtatattta tgggaccaaa
     4441 caaaagaagg aactcctcct gatggttttt atattgggac agaatataca cgagaagata
     4501 taaatcgagc tatcgcagaa aatgacccta gtctatctca ggatgaagtt ggtcaaggaa
     4561 ctatgttgag tgggatatgt gcagggcttg gaaatgtaaa tagtgaatat gcaggtatag
     4621 cagaagattc tgagctaata ataataaaac tcggcaagat agatgatttt tataatagtg
     4681 caatgctgtt tgctgcatct cagtatgcat ataaaaaagc ttttgaactt ggaagaccat
     4741 tagttataaa tatgtcttta ggtaccagta gcctagcagg tttaaccaat aggagtaata
     4801 gtgaaaaagc gttctttact agagggttat gtataacggc aggagcagga aatgagggca
     4861 atacacagac acatacatct ggaataatac catatgttgg aggttctgtt gaagttgaat
     4921 tagagttaaa tgaagatgag gaagaattat cattagaact ttggttaaat agaccagata
     4981 aagctgatgt aataatagta tctcccactg gtgaagaaag taaaagtgtt ggaatttcaa
     5041 attataataa agtaacaggt ttatttgatt tagaggggac tgaatattct ataacatata
     5101 tatacccaac aactttttct ggacagcaat ttactaatgt tactttaaag aatgcaaaaa
     5161 gaggtgtatg gaagattcga ttagtaggag tttatattat aacaggaaga tataacttat
     5221 accttccaaa tagagaactt ttaaaaagtg gaactagatt tagagaggta gacccttttt
     5281 acactataaa ttatcctgcg attcaagatg atttaataac cataggagcc tacaatacta
     5341 ttaatggtag tttatggcaa agttcatcaa gagggccaac tattgaagac aggttaaaac
     5401 cagatatagt agcaccaggt gttaatataa tagctgctta tcctgggaac acgtatgcaa
     5461 ctataactgg tacagcagca gcaagtgcac atgcagctgg tgctgctgca atgtattttc
     5521 aatatacttt tgttgacggt agatatccaa accaagcata tgtgcaaaaa ataaaaactt
     5581 ttatgcaagc tggtgctaga aaagattcaa acacagtata tccaaataca aattctggat
     5641 atgggctctt ggatgttaga ggaatgtttg atgttttaag ataggaggga tttatggaaa
     5701 aatcttattg tataatttat caaggtgata tagaatctgc cctacaagaa aatggaataa
     5761 ataggtatat ggttttaaat agtcagcttg cagtaatata tgtaccttta gattttgatg
     5821 aaactatttt aaataatata atacaagttg cttggtggga agaatctgaa ccaatgagca
     5881 gtctaattga aataactaat aatgtaaata atggagaaac cattacaaca gcagctgaaa
     5941 ctgattatat ttatgaaaat ccatataatg atataacagg gagaggaatt ttattagcag
     6001 ttatagattc tgggatagat tatttgcatc ctgattttat aaatgatgat ggaactagta
     6061 aagttttata tttatgggac caagaagcaa atacaaatcc acctccagaa ggttttattt
     6121 ttggaagtga atttacaaga agtgagttaa atatagccat aaatagaaat gatgggagtt
     6181 tgagtcaaga taatataggt actggaactt tagtttctgg tatattagtt ggaaatggaa
     6241 ggataaattc tcagtacaga ggtattacta cagaatcaga cttaatagta gttaaattaa
     6301 aatcatatac agatacttat tatgctggaa ggataaatta cagtgtttca gattttttag
     6361 cagctataac gtatgttact aacatagcaa gaacagaaaa taaaccactg ataataaatt
     6421 tgactattgg agttaagtca agtgctgtag caactacttc aatactagat acttttaata
     6481 tattgagtag tgctggtgtg gtagtagtga gtggtgcagg aaatcaaggg aatacagata
     6541 ttcattattc tggcagattt agcagtgtgg gagaagttca agatgtaata atacaagatg
     6601 gagatgacta tgctcttgat ataacactaa atacgaatgg tccagataaa gtgggagcac
     6661 agataatatc tccttcaggt gaagttagtc atgatataag gtattctcca gacttttata
     6721 tatatagagg aaaatttaac ttagaaaata ctacttatgc aatgagattt atttatccat
     6781 atattacctc tggaaaagaa aatttggaga taagattaag agatataaaa cctggagtat
     6841 ggattttaag attgacttca gaacttataa taaatggaga atatgacata tacttgccaa
     6901 ataaaaattt gatagctcca gacacaaggt ttttagaccc tgattcagta gctactataa
     6961 ctatgtatgc tgctagtgat gatgtaatta cagttggtac atttaacaat aagactgata
     7021 gtatgtggat tggttcttcg aaaggaccaa ttagaggaag aggaattaag ccagatatag
     7081 tagcttcagg tgtagatata atatccacat ataaaaatgg aacatataat acaggaacag
     7141 gaacaggagt gagcagttct atagtcacag gtgttttagc cttattgatg gaatacttag
     7201 aaaaacagga taatgtaccg agattatcat tgtttacaca agttttaaaa acatatttaa
     7261 tattgggtgc tacaaaactt gagatatata catatccaaa tgtatcgcag ggatatggga
     7321 ttttaaattt aaaaaatacg attcaacaga tagcaaatac tctatagata agaacctatg
     7381 taaaattata attgattatt agtttaattg tatcaaatac tttagataga gtagaaaaat
     7441 agcataaaaa ataaatatga aaattaaaaa acagttttat attttgaata cacaaagtaa
     7501 atattttgta tataaatagg tataaattaa gtattgaaaa gatatgatgt taatttaaaa
     7561 ataattataa tttagactta tgaattttat ttgtaaaatt gctaaaacta ttacaaaata
     7621 aataatagag gtgatagttt tggaaagtaa aaaggcaaga gcattaaaag gtaaagataa
     7681 taaaagactt aaaagttcac ttttagttgg aaatgagtcg acagcagcat gggctgatgt
     7741 tgaaaaatta aaacctcata gtaaagtttc aataccttca attaagggtg tcgaagaagc
     7801 aaaagactgg gttgacaatg gaagtaaatt ataaaatttt ggaaattgaa gtaataaaga
     7861 agtaatgaag ataagtattt attatcatta tgtaaggtag aattattata ttttaaataa
     7921 caatcagtat caattatata aatttattga ctaaaaattt ataaattggt ataattttgt
     7981 taattagcag aaataataaa agataaatac tgtgaagaga agagtagata taaaatgtag
     8041 ttctagcgag ttgaggatgg tgaaagctca atacgaggtt tatattgaag aacatcttgg
     8101 agtttctaat cgaaagctta tttagtaagt agacttagac ggaaggcacc gttataggct
     8161 ttaagcattg cttaactaag aggtcattat gacaactagg gtggtaccgc gagagtaata
     8221 gtctttcgtc cctttatata gggatgaagg gcttttttta ttgaaattat atttaaaaga
     8281 aattaataac aattatacta ggaggattta aaatgcaaaa ggaatttata acagtaaaag
     8341 aattgtacag agaaaaagaa cagtacatag ggaagactgt aaaagtagct ggatggataa
     8401 gaacatctag agtatctaaa aattttggat ttatagagct aaatgatgga agcttcttta
     8461 aaaatatgca gatagtattt gatgagaaag tagaaaattt taaagaggta ggaaaattag
     8521 caataagctc atctatactt gtagagggag agttagtatc tacagaaggg gctaaacaac
     8581 cagtcgaaat acatgcaaaa agcatagttg tagaagggga atcagatagt tcatatccac
     8641 tacaaaagaa gagacatacg cttgaatatt taagaacgat agcacattta agaccaagaa
     8701 gtaatacttt ttcagctgta tttagagtaa gatcaatagc tgcctatgca atacacaaat
     8761 tcttccaaga aagaaacttt gtatatgcac attctccaat cataacaggt agtgactgtg
     8821 aaggtgcagg agagatgttt agaataacaa ctatggattt aaatgatatt ccaaaaactg
     8881 aagatggcaa aatagactat actcaagatt tctttggaaa agaggctaat ttaacagtta
     8941 gtggtcaatt aaatgccgag ataatggcac tttcttttag aaacgtttat acttttggac
     9001 ctacatttag agctgaaaac tcttatacag gaagacatgc ttcagaattt tggatgatag
     9061 agccagaaat agtttttgca gaccttgaag ataacatgga acttgcagaa gatatgataa
     9121 aatatgttat aagctatgta ttagaaaatg ctcctgaaga aatggaattt tttaatagtt
     9181 ttatagataa aggattgtta gaaaggttaa ataagatagt aaactctgag tttattagaa
     9241 taacgtatac taaagcagtg gaattattaa ttgaatctgg gcatgaattt gagtatcctg
     9301 ttgagtgggg atgtgacctt caaactgagc atgaaagata tataacggag caaatatata
     9361 attgtccagt gtttgttaca gattatccaa aagatataaa agctttctat atgagaatga
     9421 atgaagatgg taaaactgtt agagcaatgg atttactagt tcctggagtt ggagaaatcg
     9481 taggaggttc tcaaagagaa gaaagagaag atgtattgtt agatagaatt aatgaaatgg
     9541 gattaagaga agaagattat tggtggtatt tagaacttag aaagtttggt actgctactc
     9601 actctggatt tggtcttgga tttgaaagaa taattatgta tatgacaggt atgtctaaca
     9661 taagagacgt tatacctttc ccaagaactc ctaaaaatgc tgaattttaa taattatata
     9721 taataaaact tatataactt taagtataaa cactaaaatg acttgcatat ttatctattt
     9781 tagagatatg caagttattt ttattgtact ataaatatat aatttgatta cttttatttt
     9841 gcgatataat acttataggt ttatgtaata gtatgtaacc ttttaataaa atgcaacaca
     9901 attacgtccc tgttcttttg cagtatatag agccttatca caattactta taaactcatt
     9961 taatgttatt gtctttccaa ttttctttgt ataaactcca atactaacag ttacacattt
    10021 taaaggaaca tcatcacgct caaaattgca ggactctata atttttcgta aacattctga
    10081 tttaagttta gcatctgcct cattacagtt aaaaatacat ataaaaaatt cctcaccacc
    10141 atatcgtata gcatgttcat tttcagaact tgtaaaatct aaaattactt tggcaatatt
    10201 ttgtagacaa tagtctccct tttgatgtcc ataattatca ttaaattgtt taaaataatc
    10261 tatatctatc atcattccag ttataggttc ttgatttgca tggctgatta attttttttg
    10321 tagaatatct tcaaggtatc tacggttgtt cattttagta aggtcatctg tcataacttt
    10381 gtcgttaagt aaattattta tatgagatat ttcttcatat tgttgactaa ttaaaagttt
    10441 atcatagtaa gtaaatacac gatttctata tagcattgta gaaacaaaaa tggcaaggga
    10501 tgttataaaa aatgtgttga caatattact aaactcattg cctggtatag tttgaatccc
    10561 aggtaagaat aaattaagaa acaaaaagca tcctccaaaa actattactg attctccaaa
    10621 ttttaatact gagaaagctg cagctgctaa cataatataa ctaaatacac ttaggtcatt
    10681 tccacctaac tggtcaagta gagtaattac acaaccccat aggcagaaaa aagtcacata
    10741 aattatccat aaatttatat aaaacgtaca gcaatccttt tttttattcc aaaggtaaga
    10801 aaatacaaac ataaagacag aagatacaca aaatagtgtt acatacatat aaaaatacca
    10861 ttggcgtctt ggtgtgacaa aagggcctcc ttctctagct gagattgata tcatcataat
    10921 tatttgagat attactatta ttatgcaaat gattttaccc gtatgataaa gataattgca
    10981 gagttctttc tcaacattgt ctttgtgttt tttgtctaga ggaaatccaa aaaatttttt
    11041 tattttttct aagttcatat aagcctccat taaaaataaa gcataaaaat aagtattcct
    11101 ttctatcatt agatatttgg aatacttcgt ttatgattta attatatcaa aatatattgt
    11161 gtgtaacaat ctaaaattgt gatagataaa atttattaga taatgtcgag taactttatt
    11221 gattaatact ttattataat taagatttac agatagttta ctactgaaaa taaagaatag
    11281 aataaattta aattaagcat aataaaatat aaataataaa gttcgcatta acaaactatt
    11341 taaatatcaa gacctgttaa aaaacaacag caataaacaa ataaaataga taaatatatc
    11401 caattaaggt ataattaagt tttatatgat atagttatat ttgataattt acaatagtaa
    11461 aacttggagg gaaaaaatgg gtgaaaagaa aataaataat atgaattatt cacataataa
    11521 ttattccaaa aagacaggtg aaccaactag aaaaataaaa aatagccata tcactcttaa
    11581 aagtaaagaa gaaaaaattt gttctagttg caataaagaa aataatacta aggataatta
    11641 ctgtaaattt tgtggaaatg aactttatga aatagcatca ttaagaccac ttgaaacaaa
    11701 actagacttg aaatctaaaa taaaagagct ttcatatcat gcaaataaaa gaggtgtatt
    11761 tttaacaaca tttacaacca tatttatatt gtttattatt gcattgatat ttaaagctat
    11821 tataactatt caatttaatg atattagtta tttagtaaat ccagcacata taatcctagc
    11881 tctcaacttg ggtcaaatta gtgtatctat gtctaccatg atgggttctg ggtttataaa
    11941 tgccaatata ggtttgttga tattacttat aatccctatt ttggtattat taatatcaaa
    12001 cctaattttt atgagaaaaa gatgtagaga ttcaaaaact gtattagcaa attctctagg
    12061 agttggaatt ttttatggat tagttttagc tattttatct atttttacta atgtaaaaac
    12121 tagttcacat agtatgttag aatatggata tgcattagaa tatagttatg aattttttag
    12181 tgttctttta aatggattcg tattagggtt tatatgtaca tacataacta cttataaaaa
    12241 aagttatgaa aaagaaaata tgtatttatc tcttttttca aattctataa gaacattcat
    12301 attaggttat gttttagtat tagtaatact attggtacta actatatctg atagtagtta
    12361 cttaaatgaa ctagatatga gtagttattc aaatggatta aatttattta ctatactccc
    12421 acaaatagct agttatatgt gggcttttgc aaatggaatt tcagtaacca taataaactc
    12481 aacagtatca atgtttactt taagttcttc atctttattt ggagatacta aattaatgtt
    12541 ttatgcaatg ggagcattgt ctatgttaat attattatta aatggataca agctaagatt
    12601 taagtataat acagatagta taagacctat aatagtattt agtatatatt atgcattttt
    12661 gatgggaata ttggccttat ttagtacatt tatactagat agtaatatta atttctttaa
    12721 tacaactaat tatggaacaa ctttaataat gcaatttaaa gtcttacaag ctattgtaat
    12781 ttcatttgta tatagttttg tgatatcttt aataggatat aagctaaatt cagctgatta
    12841 aatatgattg gagggaattt ctgtggaagg aaatactaat aataaagtag atgataaatt
    12901 ttctaaggtt aagaaaaagt ttggtagtgg aaaatttaaa gttgaattat ataaagaaaa
    12961 atcaaaatta aatagagaga ttgatgatat tcaagaaaag aaagcaaaga tattcttgga
    13021 aatgggaata ctgacatatc aaaaaattag agaagattct attaatgata atagttttga
    13081 taaattctgt gatgagttac tagaacttga taagttaatt tatgaaaaaa atatggaaat
    13141 taatgaaatg gaaatggcag aaagtaatgt aacttgtgag tgtggttatg ttggaaatgt
    13201 aaatgataaa tattgtgctg aatgtggtaa aaaatttgaa ttggaaaatg aatgtgatga
    13261 ttttataatt tgtggttatt gtgaaagtga gatagataca gaggctgaat tttgtccatg
    13321 ctgtggaaga aaaattataa tggatttaga ataatattgg gaaacctatg tatagtatac
    13381 atgggttttt ttattgaaat tatttaaatg gacttataac agtagttata taaaatgaaa
    13441 ataaacacca aaaaaatgaa ataaaatttc ttttaaatgt aaaaaacaag ctaatatata
    13501 aaataaatca gtaaaaatat agatgcgaga aaaaatatga aaaaatattg caaagaaaaa
    13561 gttttcatgt tattattaat acataaatca agaaaataaa tttcagaaac gaggtaattt
    13621 aaaaaatgct agataaggta aagggaagat taatagtatc atgccaagct ttagaaaatg
    13681 aacctcttca tagtcctttt ataatgggga gaatggctaa ggctgctatg gaaggtggag
    13741 ctgttggaat tagagctcaa ggagttgaag atattattga aataaaaaaa gttactggac
    13801 ttcctgttat aggtattata aagagaaact atgaagattc agatatatat ataacgccaa
    13861 ctaaaaaaga agttgatgaa cttttaacaa cagggtgtga aatgatagca ttagatgcta
    13921 ccaatagagt tagaccaaat aatgaagact taaaagaact tataaagtat ataaaagaaa
    13981 acggtgttct agtaatggca gatatttcaa attatgatga ggctatcaaa gcacaagagt
    14041 acggtgtaga ttgcgtttca actacactat ctggatatac tccatatact aaaacattag
    14101 aaggtccaga ttttgtatta atgaaaagat tagttaaaga tttagaaata cctgtaatag
    14161 ctgaaggaaa ggtaaatact cctcaagatt taaaaaaggt ctttgagttg ggtgttcatt
    14221 catcagtagt aggttcagca ataacaagac cacaattaat aacagaaaaa tttgttaaag
    14281 caattgaaat taattaaaaa ttaatttata aattaaaaaa ttattatatt taactatatt
    14341 tgaaaattaa aattggaggt aaagatatga gaacaacaga tatgaaggga atttattcag
    14401 cattattagt ttcatttgat aaggaaggaa atataaatga aaaagggtta agacaaataa
    14461 ttagacacaa tatagatgtt tgtaaagtag atggtcttta tgtaggagga agtacagggg
    14521 aaaacttcat gctttcaact gatgaaaaga aaagaatatt tgaaatagct aaagatgaag
    14581 tgaaagaaga aataaaatta atagctcaag ttggttcagt taatttaaaa gaagctgtag
    14641 aacttgctaa atttacaact gatttaggat atgatgcaat aagtgctgtt acaccttttt
    14701 actataaatt tgattttgaa gaaataaaac actattataa tacaataata aattctgtag
    14761 ataatagatt aataatatac tctataccat ttttaactgg tgttgatatg agtttagacc
    14821 agtttggaga attatttgaa aatgaaaaaa taataggtgt taagtttaca gcagcagatt
    14881 tctacttatt agagagaatg agaaaaacat tcccaaataa attaatattt gcaggatttg
    14941 atgaaatgat gttaccagct acagttttag gtgtagatgg ggcaattggt tctacattca
    15001 atgtaaacgg tgtcagagct agacaaatat tcgaattaac taagaatgaa aaaatatcag
    15061 aagcacttga agtacaacat gtgactaatg atttaataac agatatatta ggaaatggct
    15121 tatatcaaac aattaaatta ctacttgaag aacaaggtgt tgaggctgga tactgtagac
    15181 aacctatgaa agaagcaact gatgaaatga aatcaagagc aaaagaaata tacagaaagt
    15241 atttttaata aaatactttt agtatgggag gattagtcaa atggtaggat ttacttggat
    15301 agattttgca atactagtgg tttacttatt ggcagtttta tttgcaggat tgttattttc
    15361 aaaaaaggaa atgaagggaa aagagttctt caaaggtgat ggaactattc cgtggtgggt
    15421 tacatcagta tcaatatttg ccacattatt aagtccaata tcatttttat cattagcagg
    15481 aaattcttat ggtggtactt ggattttgtg gtttgcacaa ttaggtatgt ttatagcaat
    15541 acctttgaca ataaaatttt tcttacctct atatagtagg ttagaaatag atacagcata
    15601 tgaatactta gaaatgagat acgaaagtaa aggtcttaga gtacttggag ctttgatgtt
    15661 tataatatat caaataggac gtatgtctat tataatgtat cttccatcaa tagttctggc
    15721 tggtcttaca ggaatacctg taaatgtact gataatagtt atgggtgtaa tagcaataat
    15781 ttactcatat acaggtggat taaaggctgt cttgtggacc gattttatac aaggaatggt
    15841 attaatagta ggtgttacag ggacattgtt ctatttaata gcaagtataa atggtggttt
    15901 tggaacagta atggatactc ttacaactgg acacaagttc ttagcaagta atgaagttat
    15961 gtttgataaa aatatactat ctacaagtgc atttataata tttgttggag ctggtcttaa
    16021 tactttttca tcatatgtat cgagtcaaga tatagttcaa aggtttacta caacaactga
    16081 tataaagcaa cttaataaaa tgacatatgg aaatggtgtt ttatcaatgg gacttgcaac
    16141 agtattttat ctaattggaa cttgtttatt tatatactat actcaaaacc cagatttagc
    16201 tcaaacagtt caacaagacc aagtatttgc atcatatata gcttatgaat taccagtggg
    16261 tataacagga atactacttg cagcgatata tgcagcttct caatcaactt tatcgacagg
    16321 gcttaattca gttgctacaa gttggacttt agatatacaa actatcataa caaaagatat
    16381 aagtatggaa aagcaaacta agatagcaca atatatatca ttaggtgtag gtatactttc
    16441 tatagctgtt gcaatagtat tagcaaatgg agaaataaag tcagcatatg aatggttcaa
    16501 tagttttatg gggctagtac taggtgtttt agcaggaata tttgtacttg gtgcattttg
    16561 taaaaaagca actaaaatgg gagcatatgt tggattcata gtatcagcaa ttttagttgt
    16621 ttatttaaaa taccgtatgc cagaagtaac ttcttgggca tattctctaa taacaatcac
    16681 gacttctgta gtggtaggta caatagttag tgcaatagaa gctaaagtta aagcaaaagt
    16741 atctttacca aaagctgaga ctactgttta ttatgaaaaa taatatttaa atgataaatt
    16801 gtcttataga gtgattagat aaataattta gttactctat aagattgtat aatatacttt
    16861 gtatacaaaa gtataaaaat agactttata attatatagg gagatagtgc ttatgatatt
    16921 tggaaatata aatcatagta agacatattc aacattgcat gaagatttac ttagatgttt
    16981 tgaatatgct aaagataatt cattaataga ttatgaaaaa ggtacttata caatagatgg
    17041 tgatgatatt tttgtgaata ttgtagagta taaaacttgt gaaaaagaag atagattctg
    17101 ggaagctcat aagaagtata tagatgttca tttgatgctt gatggatgtg aacgaataga
    17161 tataaatttt atagaaaatt tagaacaaaa aatatatgaa gaaaagggcg atttcttgcc
    17221 tcttgatggt aaaaataatg gatacatgga acttagaaaa ggtgattttt tagtttgtta
    17281 tccggaagat gctcatatga caggaataaa agttttagaa aaagaaaata tcaaaaaagc
    17341 tatttttaag gttttggtta gataatgata atttatataa ccactagggc tgaaaatagt
    17401 gatttaactt tcagcgatta gtgagacttc gtaaatttta gttagattac tattttcagc
    17461 cttagttttt tatgagaaaa ttaaaaataa ttaaaagctt aaaattttgt aaaggagtta
    17521 acttatggaa aatatagatg tattcttatt aataggacaa agcaatgcta gaggattggg
    17581 aaatccaaaa gagagcatca ttccaaatga aaattgtttt gaatatttga gtactgatga
    17641 aattataaat atgagatgtg agctagagac ttcagaaggt gatggtacta tagcacctgc
    17701 attttcaaat gaatggaata agcttactgg aaataaagtt tgttttattc acaatgcaaa
    17761 agatggttca agaataaaaa actggaatca tgataataac tggtttctaa atgacactat
    17821 agaaaaattt aatacaggct gtactacctt aagtaaacat tataatatag aaaataaata
    17881 cgttatttgg attcaaggtg aaagtgatgc aaaatatggt agtgatgtat tgtactataa
    17941 agaaagtcta aaaaaaattg cgtataggct taaagaagaa tgtatgattg ataaaatgtt
    18001 tgtaagttta actggttatt ggcttggaga agatgaatat tttattagaa caagacgaat
    18061 tgcagcagca caggagtcgg cttgtagtga atgtgatata ttatgtgtag gaagcaaaat
    18121 tgctatgcag tttcatgaca gaggattaac tattgatgat gttcattaca ctcaagaagg
    18181 tcttaatatg ttaggagagg acttatgtaa aaatatttat aagtatcata taaccaaaga
    18241 aaaaagtgta ttaagtgata ctatagactt atcagaagca aggaaatata tctgtgaatt
    18301 ggaaaagata agtaaaagat ttgtgtaata taaaatttta aatagagttg ttaatttata
    18361 aaaataattt gtatggagat atgttatgaa aaattacgtt tgtatagatg ttggaggtac
    18421 ttcaataaaa tatggtttta ttagagaaga tggatttata attgataaat caagtcttga
    18481 tactgaagca aaggaaaaag gtggagaagg aatattagcc aaaataaaag atatagtaaa
    18541 aaaatatata gaggaaaatg aaataagtgg tatatgtata tcaactgcag gtatggttga
    18601 cccagttgaa ggtaaaatac tatttgcatt agaagaatta attccaaact ataaggacat
    18661 gcaattaaaa aaagaagtag aaaaagagtt taatataaaa tgtgaagttg aaaatgatgt
    18721 taattgtgct ggacttggtg aaatgtggct tggagcaggt agaggtgcaa catcttctat
    18781 atgtttaaca ataggtactg gaattggtgg atgtattata ataaataata aactgataaa
    18841 tgggtttagt aatagtgctg gtgaagtagg atatatgaat gtaaatggaa gttcttttca
    18901 agaattggca tctactagca gtctaattaa aaaagtagca aaaataaaaa atttgaatga
    18961 aaatgactta aatggaaaaa taatttttga tttagcaaag aataatgatg aagattgttt
    19021 aaaagaactc gataatatga taaaatcatt agcagttgga atcgcaaata tatgttatgt
    19081 aataaatcca gaagtggtaa ttttaggtgg tgggattatg gcacaagaaa agttcctaaa
    19141 accaaaaata gacaaagcat taaaggaggt tcttatagag agagtttaca aaaacactaa
    19201 tatagaattt gcaaagagac aaaatgatgc aggaatgctt ggagcattat ataatttttt
    19261 aaacaaaata taaagaatag aagggttttt aatgagtatt ttagaacaat tggaatcacc
    19321 aacttttaaa gtcacaaaat cagataaatt attaatagct tatataaaag aaaatataga
    19381 tgatgtattt tataaaccaa tatcacaaat agctaaagag agcaatattg gtgaagcaac
    19441 tataactagg tttgtaaaaa aaatgaattt taatggatta caggatttta aagttacttt
    19501 agcacaagaa atatcaacta tcaataaaaa aaatattata aataaaaata tacaaaatga
    19561 tgagccagct ttggatacag caaaaaaact tttaagttca aatgtaacca ctttagagaa
    19621 cacagtagag ataatcaata gtaaggatgt acatgactgc gcaaggctta taataaatgc
    19681 aaagaaagtt tacttcatag gtattggata ttctggtata atagcacagg attcaaacta
    19741 taaatttatg agaataggtc ttaattgtgt aagttttgac agtagtcata caatgataat
    19801 gatgtcttcc ataatggaag aaggagattt aataattgca atttcacatt ctggtgaaac
    19861 agaggagatt ataaaaactg taaagcttgc aagagccaat aatgctaaaa ttatatcaat
    19921 tactgaaaat aaaaattctg aattaaagga tatttcagat gtacacttat cttatgtatc
    19981 gggagaaaca gtactggaaa ccggttcaat atcttctaaa ttagcacaat ttttcattat
    20041 tgatttagta tatacacagg tagtaaaaga actttctaat gaagctattg aaaggaaaat
    20101 taaaactaca aatgctataa aattgtttaa aaatgaataa aataagaaat ttgtaatgtt
    20161 ttatagagct ctcttaaaaa tttaggaatg gagagatatt acatgtctaa attaaaagat
    20221 tataatattg ataaagtaat aatttttaat aatttaagta ctaaaacgaa gaatcaactt
    20281 aaagaaaaag ttaaactaaa aagattacaa aaaaaagaaa ttttatttta tgaaaaagat
    20341 agcttagaca gtgtttatat acttttagaa ggaaaggtat ctatttttaa gataagtgaa
    20401 aatggagaga gaaaagtaat tttcatatta aatagtgggg aagtcataaa tgatattaca
    20461 cttgacgctt ctaaaaactc tactgttggc tgtgaagcat ttgaaaattc aattgtagct
    20521 tattatagta ttgaagaatt tttagaaatt atgcaaaatg attttcaact tacaaaggat
    20581 attatttctt atatggaaag aagaataaga cgtttatata gacagttgaa aaattctata
    20641 tctataaaag tagataaaaa acttgcagca aaattatata ggttaagtag agaatttgga
    20701 gtttgctgtg gagaatggac tttactaaat gtaaatatta cagttacata tttagctgat
    20761 atgcttggct gtaaaaggga gactgtttcc agaatgataa aggtattgca aaaagaagaa
    20821 cttattaaaa ttgacaataa aggtttttat gttaaagaaa ctgaattatc aaggtatttt
    20881 aaaaataatt aattatgatt aagtaagagt attattagaa ttttaattaa ataaaaatat
    20941 tattaagaga atctatttat agattctttt ttattgtcta atttattaat ttttatttta
    21001 ccctattgaa ttgtataaaa aaacaaataa gattcttttt tattcaaaaa acttactaaa
    21061 tttttgatta tcttttcata agttggtaaa tgccgatata tcccgtattt gagggcttta
    21121 tcggtatttt ctttttggaa gataccttgc ataagctggc atttaccagc atgaaaatgc
    21181 aaccaaaagt agtaaatgag tagtaaatat acgttccgat attaacaagc cagtctttcc
    21241 agttctgctt ttgcagattg gaatgtaccg tgtgcgtagt agccaagcgt catggttatg
    21301 ttagcgtgtc ccatgagata ttgcagggtg ttcgggttca ttcccttgtt tgccatattc
    21361 gtacaatagg tatgtcggaa tgaatgtggt gtaatgtttg gtaacttgtc tttgtggtat
    21421 ttgttatact tcttaatcag tcctcgtacc atacccacat agtttccagc gactttaggc
    21481 aatccctcac ggtttaagaa taggaaattg ctgtaaccac caacaaccag cggttgagct
    21541 tttactctgt tctttagtat tctttggagt gcttgataag ccctttccgt caatggaatt
    21601 tttcgttttc cgttctttgt ttttggagtg tcaatatagt agccgatttc gctatctctt
    21661 aatagctggt ggtctatatt gagtattctg ttctgcatat caatatgcgt tgtcagtccg
    21721 caaaattcag aaatacgaag tcccgtttcc agaagaagca caacctcatc ataatacttg
    21781 ctgtaaatct tatccgtttc cataaaggca agcaggcgtt cttcctgctc tggcgtaaga
    21841 ataactttcg gctcggtatc atcttccaga acatcactta gcttaaattc aaacgggttc
    21901 tttctgatac agtcgtcttg tatcgccata taaaatgacg ctttcaagga acgcttatag
    21961 ttgtcaatcg ttttgtaggc atatcctctt tcactcattc tgatagccca ctctttagcg
    22021 tccgactgtt tgacggtatc aatcgctctc atacccaatg ggtcattttt cagagcgttc
    22081 ataagatact gtcgtccttt ttccgtagct cgcttgatgt tctttctttg gctgttcttc
    22141 ttgtcgtaaa gctggcagac tgtcatttta ccgcctgccg tgtcaatacc gtcatcaagg
    22201 tcttttttta tctgtgcttc tttttccctc aacgatatat catcacgttt tccagcaggc
    22261 gttttgtctg tcggtacaag tttccaagca taaataaact gtggctttcc gtatatatcg
    22321 gtatatttat aaacgtatct tccgtctttt cgctggctct ctccattacg caaattgcga
    22381 tttttactgt cttttcgttt cacattagac atagtgtaaa gctcctttcc gtcatggaat
    22441 gagccgtgat acgctatact tattatacca tatccacggc tctatgacat tagatttcgt
    22501 ccaatgtatc tatgattttt tcaaattgtt ttcgctttat ctgaatacgg ttgccgttca
    22561 cgataaccca gccagcgtca atattttctt cggctaattt acgcaacttc ttttctccaa
    22621 tgcggaagta tttggacgct tcttcaatcg tcagtgtata tttttcccag ataggcacat
    22681 cagtattatt cataatttat gcaccccctt tactgtgtgt cagtctataa gttatataaa
    22741 caggcagacg gctttggaaa gctccattag tatttcaact attcccattc ttgtgggcag
    22801 aaccgcacca tgcggaagta tcattatgct gtaagggtag gtcatggcaa aacgactttt
    22861 ccaacggtcg ctctcggatc actgcgtggg tcgctcgctt tcttttggaa aggtcatggc
    22921 gtacagttcc cgtggctctc tatccttaca aacgtatctg tctgctttat tcagttttca
    22981 aagagctgtg gcgaaagcgg tatgctttca tgtaaaagca ggagcagagc aggaaagagg
    23041 ggattgaaca aatcatgctc tgcgttactg ctggttattc ttcttcaatt tctaaggcaa
    23101 tatcaaaccc taaaatcatt ttgataagtg cttctcggat aagtcctttt aactccatat
    23161 ctacaacgat ataaacatta ccgtattcat catagagtgg acgcaaacag cacttcgata
    23221 tgtaagggtc ataaaatgca agtacctttt caatcgcttt ttcgtccccg tccatagctt
    23281 gtgaaagcaa gtagtaagac ggcttcttaa atgtgtgttt catttgctat cgtttctcct
    23341 ttaatagttt tctcataagt gccaatgaat tgtggcggtt ctgaaaaact ccgcttctgg
    23401 aaatgttttg ctttttggcg atttcttcat tgttcatttc caaaaagtaa tacatcaaca
    23461 aagtttctcg gttacgttcg gataactgtt tcaaggcttc tgccaattca tcatcataga
    23521 cacgaatatc attaccgcat acattgaaaa tcgtatagtc tgtttcgtag tcgtcccata
    23581 cagccaagtt ttcaaccatg atttctggaa gttcacaaaa ggaaatttct ttttcttttc
    23641 ttcgcttcaa tccctgctga tagtccttga caacatgacg gacaactttc ttcaaacagc
    23701 tttcaaaacg gcattgaaca gttttctgga agtcagacgg tttcatcaat ctcaccccct
    23761 ttccgttatg acgtgaaaag tgtttatccc tctttccgca ccatataggg aatggcaagt
    23821 gtgatttgtt aagttgaaac cagaaaaaat cgaaaaaact ttttagtaaa acaaaaacag
    23881 cccgtgtgaa tgtgaataca caaacggact gctttataaa tgctatctgt atgaattttt
    23941 aactcttgta gagggtagaa tgattgtaat actgaactaa aatatttttt cagttgggaa
    24001 cagcaaagta ttattccata tacaatcatg gcgtagcccc ctttaccaga gctatacctt
    24061 gtgccaccaa tgaaaaagga cggctctggc tatatccaaa accgccccgt tatgttttca
    24121 agacgcacaa aagtatatca tctcggaacg gttatggtga tttaaaatga gaaaaacaaa
    24181 agaaacacat acttttgatt tcagaccgct agggctggca atcagagaag cccgtgaaaa
    24241 agctgggctt tctcgtaatg att
//
LOCUS       DAMJWU010000047        24262 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMJWU010000047 DAMJWU010000000
VERSION     DAMJWU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.047"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(17..553)
                     /locus_tag="PC460_002125"
     CDS             complement(17..553)
                     /locus_tag="PC460_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2601009.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(666..1340)
                     /locus_tag="PC460_002126"
     CDS             complement(666..1340)
                     /locus_tag="PC460_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2601010.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            1543..3417
                     /gene="asnB"
                     /locus_tag="PC460_002127"
     CDS             1543..3417
                     /gene="asnB"
                     /locus_tag="PC460_002127"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2601011.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(3509..4945)
                     /locus_tag="PC460_002128"
     CDS             complement(3509..4945)
                     /locus_tag="PC460_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2601012.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            5274..5852
                     /locus_tag="PC460_002129"
     CDS             5274..5852
                     /locus_tag="PC460_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2601013.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            5891..6469
                     /locus_tag="PC460_002130"
     CDS             5891..6469
                     /locus_tag="PC460_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2601014.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            6555..7184
                     /locus_tag="PC460_002131"
     CDS             6555..7184
                     /locus_tag="PC460_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2601015.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            7174..9933
                     /locus_tag="PC460_002132"
     CDS             7174..9933
                     /locus_tag="PC460_002132"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2601016.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            10020..11582
                     /locus_tag="PC460_002133"
     CDS             10020..11582
                     /locus_tag="PC460_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2601017.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            11583..12719
                     /locus_tag="PC460_002134"
     CDS             11583..12719
                     /locus_tag="PC460_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2601018.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            12783..13859
                     /locus_tag="PC460_002135"
     CDS             12783..13859
                     /locus_tag="PC460_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2601019.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            13837..15075
                     /locus_tag="PC460_002136"
     CDS             13837..15075
                     /locus_tag="PC460_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2601020.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            15129..16217
                     /locus_tag="PC460_002137"
     CDS             15129..16217
                     /locus_tag="PC460_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2601021.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLISKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            16245..17036
                     /locus_tag="PC460_002138"
     CDS             16245..17036
                     /locus_tag="PC460_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2601022.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(17151..17705)
                     /locus_tag="PC460_002139"
     CDS             complement(17151..17705)
                     /locus_tag="PC460_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2601023.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            17901..19103
                     /locus_tag="PC460_002140"
     CDS             17901..19103
                     /locus_tag="PC460_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2601024.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            19160..20140
                     /locus_tag="PC460_002141"
     CDS             19160..20140
                     /locus_tag="PC460_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2601025.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            20388..21341
                     /locus_tag="PC460_002142"
     CDS             20388..21341
                     /locus_tag="PC460_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2601026.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            21331..22287
                     /locus_tag="PC460_002143"
     CDS             21331..22287
                     /locus_tag="PC460_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2601027.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            22284..23039
                     /locus_tag="PC460_002144"
     CDS             22284..23039
                     /locus_tag="PC460_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2601028.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            23131..24096
                     /locus_tag="PC460_002145"
     CDS             23131..24096
                     /locus_tag="PC460_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2601029.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         9579 a         2550 c         4136 g         7997 t
ORIGIN      
        1 tttatttttt ctacaactat aataaaatat aaaattgata tccaatcaaa aaaataacta
       61 tgaaatttaa tagcttagat atgaattttt ctgtttttga gttagttttt ataggtagta
      121 aagaaatgtt tatagtaatg ggataaaaca attcaatacc ttctttagta ataaggtcaa
      181 gacacaagtg agatagatat cctattataa acccactgga caaacatgag aaaactatat
      241 tattgttagt gaagattgtc agagatttaa aaaaactata aataagtaat aaggcaatta
      301 gactgtgagt aaaccctcta tgtctaaacc tacttccaaa taatttgtat attaacataa
      361 attttttact tatgtaagaa cctctcatat ctatatctgg aaataaagaa cctaaatatg
      421 caaaataaat ataaattata attaatacta ttttatatgc aatatcgtat ttaactaggt
      481 acagattgtt tataaagggg agtatcaaaa gtgcgaatat ataacctccc ctttcatgtg
      541 tttcttttat catataaaaa tcctcctgaa tcgtcactat aaacattata ttggtaaatt
      601 tagactatct tttattatat tatatagcaa acataagttt ttttcaaaag gattttaata
      661 tttaattaat cttgagattt taaatttttg taattttcta aagcaccatt ccaatttact
      721 acattaaacc agttgtcaat gtattcattt cttctatttt gatattttaa atagtaagca
      781 tgctcccaaa catcaagtcc tattatagga gttaggtttt tacttacagg gctatcctga
      841 tttggagtag tcataataga taatttccca tctttagtag ctacaagcca agcccaacca
      901 gaaccaaaga catctaaagc agatttttgg aactcttgct taaatttttc aaaagaacca
      961 aagtctctat caatagcttc ttttaaagat tcagaaggta tggttttttc tggcgtcatt
     1021 atatcaaaaa agaatttatg attataagct ccacctgcat tatttcttac agttgtagca
     1081 atatctttag gtaaagaatc taaattttgc aataattcac ataaagaata attataaagc
     1141 tcaggatatt tttcaagagc agcatttaat ttatcaacat aagcttgata atgcttatca
     1201 tgatgcagtt tcattgtttc tttatctata taaggttcaa gtgcatcata tgcataaggt
     1261 aatggtttaa ccttaaattt gttattttca ggtgtaaaag caaatgaagt tatgcactgt
     1321 gagattataa ataaagacat aataactggt attaatattt ttttcttcat aaaaatcacc
     1381 tcctaatata gtatttctag aaataaaaaa aatatgaaat attaacagat aatttataag
     1441 aatttaatag caatttcatt ttaaattgaa taagttttaa agccaaagac aaaactatga
     1501 aatatagaat aagactaaag gacagaaagg aattttgatt atatggacgg caatatttca
     1561 atttattata aaggtgatat atctaaaaaa gaagatatta aatttaaaat aaaagaatct
     1621 gtaaataata caagactttc tagtagaata gagtataagg atgaggaaaa aacttttgat
     1681 tttgaaagat ttaatgtaat tgatgctatt tatggaaata aaccatttga aaaagatgga
     1741 aaagttattg tttttaatgg aaatatatat aactatcatg aaataaaaga agaattaatt
     1801 aaaaaaggac acaattttac tacaaatgat gatattgaag tcttattggc atcttatata
     1861 gaatttggta agaattgtgt acataagatt aaaggaatgt tcaactttat aatctatgat
     1921 agagaaaatg aaagtatatt tggagcaaga gatttatttg ggataaaacc actctattat
     1981 ataaataagg aaaatgcaat aattttttcc tcagagtata agtttttact tgaatatatg
     2041 aaaaatttaa atataaatga aagaagctta caaagttatt tttcatttca atatgtactt
     2101 cctgaagata ctatgataca aggaataagg ctgataccag caggtcacta ttttagggta
     2161 gaaaatggta tactatcttt aaagagatat aataaacttg agtttagaag tagtacaaaa
     2221 tttttttata ctaaaaatca cttaggcaat agagatgtga acgaggatga tataagaaat
     2281 atagtagtag attcaatagt aactcatatg gaagaggaga aagaaattgg aacatttctg
     2341 tctggtggaa tagattcatc tattataact actgtggctt cacaaattaa tccaaatata
     2401 aaatcttttt ctgcaggctt tagtgtcaaa ggatatagtg aattagaagt agcaaagaaa
     2461 acagctgata agttaggtat agaaaatatc cagattaata taacacaaga tgaatatata
     2521 aaatcattac cgaatgtaat ttattctttg gatgacccta tagcagaccc ttcagaagta
     2581 ggtctttatt tcttaattaa agaggcaggt aaacatgtta aagtagcatt gtctggagaa
     2641 ggagcagatg aattatttgg aggatataat atatataaag agtatagtac tatgaaatca
     2701 gttgtcaatt cacctactta tattaaaagt atattaggaa gagtttcaga acttatgcca
     2761 aacataaaag gaagaaatta tctttataga gctacaacac cattagaaaa aagatatata
     2821 ggaaatgcaa aagtttttga aaacagcgaa gtaaaaagat tcttttttaa gtataaggaa
     2881 aagaatatat atgaatacct gttgtctaat ttgtatagag atgcacaaaa aaataattac
     2941 gattatataa gtaaaatgca acatatagat gtgaatacat ggcttcaagg cgatatactt
     3001 caaaaaatta gcaaactttc tacagctgag caggttgaac ttagagtacc atttttatat
     3061 aaagatgtat ttgatgtagc aaaaaattta agaatggagc aaaaaataaa taagaataat
     3121 acaaaagttt tacttagaga ggcgtttaga gaaatagtcc cagaacatgt tgttcaaagg
     3181 aaaaaattag gatttccaac tccaataaga gtttggttaa aagattctct aggggatatt
     3241 gtaaaggaaa ctatttataa ttcaaatgta gatgaattta tagataaaaa atatgcaata
     3301 aaacttttag atacgcatct taaaggtcat agagataatt cgagaaaaat atggactata
     3361 tttacatttt gtctatggca tcaattattt attgagcata aaaatgtcga gtattaatac
     3421 ttttttcaat attaatcaga gggcaaaagc atgcaatgtt ttcaccctcc tgattattat
     3481 ggaataggat aagtagaatt ttgattgatt agtttactat ctcaataaaa ttattggcaa
     3541 atattttgac aagattactt tcaacagcta aatttaagtt ttgtccatca tcatatccag
     3601 cggaaatcaa tcctagtaag tttccaaaca tatctaaaag agctcctcct gaactaccac
     3661 tagagattgg agctgtaaat tgtatcattt gaaccgtttc aaaatcacga aaagctgaaa
     3721 taattccatc tgaaactgta ttaaaaagac ctagagggct tccaattgca actatttttt
     3781 gacctctcac aggtttttta ctgactttaa caggtattgg cttgcatttt ctatcaacct
     3841 ttatcacagc taaatcataa tctgaatggt attttataat ttggtaagat gtataaactt
     3901 tatcatcatt ttcaaatcta actaaaaatg aatacccatc attgactaca tgaagattcg
     3961 taagtatata accttcatta tttattacta ctccagaacc gctcttaaac ggtttatttt
     4021 cgttattgca cacaacaatc ataacaacag aagctgataa ctttgcaagg tcttcataag
     4081 aaagttctac ttcctcatct tgtacaattt ttttagatgt tagatttgtg ttattaagtg
     4141 tatttttaga gttaggttta ttcgtagtag gagaagtctt attggtattt tcactgtaat
     4201 ttatatttgt aatatgcctt tcaaaaggta tttctaatat atttggagtc ttcctagaaa
     4261 gttcaaatag agtagaaaat tttgaacatt caggatataa atcaagaata tctttatttg
     4321 gatttatata taaaatatca cacccaagtt gtgataaaaa atataagaaa aaaagttctt
     4381 gccttttaat atttccaaag tatactatct ttggtgaaat agtagtcttc accttgttat
     4441 aaaataattt aggtagaaaa tagtctatcc aacacaaaag tttaactgca aagtttttga
     4501 tgatagagga tgtacaattt ggcatacttg tggaaaataa atctaaagta tttttaaatg
     4561 caatcttttt agtccaattt aaggtgtcat tttctatttt acatggaaag tcgatattat
     4621 ataaatccca actgttaaac ttagaactat tataaatatt aaaaatttta agcatcttat
     4681 ctgtttgttc tttgtcaaaa tttattttta gattgtttaa tctaatatag ggaattgtag
     4741 gattattaaa aaaataagta tctatatttt taattttttc tatgtattta tcaattgaat
     4801 catcaacacc tatatatctt gtaaagtaga cttctttaat atatgaagaa cgcttatttt
     4861 tataggtagt atcagatata aattcaatta gaggttcagt agactttttt attgaaatct
     4921 ttaatggttt aatagtacaa atcatatttt ttctcctata ataagatgat ataatctatt
     4981 ataaaacaac ttaaaacaat atagtacaat aattttgtag tttcaaaata aaatatgtac
     5041 ttgtttatat aaatgatata taattacata ttaaacatag cttttatgca tttaataaag
     5101 tatgtaaaat ctatgtaaaa agtaagaaat ataaacattt ttttaattat attttaaaaa
     5161 tatcaagcaa gttttattca caaatgaata aaaaatgtta taatatgatt aaagaaaaag
     5221 ataaataatt agcaagattt gtttagctaa aattaacaag ggagcgataa attatgggta
     5281 taacattatc aaaaggccaa aaggttgatt taactaaagg aaatccaggt cttaaaaata
     5341 ttttagtagg tttaggatgg gatactaaca agtatgatgg tggatttgac tttgatttag
     5401 atacagcagc atttttaaca ggagcaagtg gaagtgttac taatgatgga gattttgtat
     5461 tttacaacaa tttaaaacat acatctggag cagttgagca tctaggagat aacagaactg
     5521 gcgaaggtga tggagatgat gaacaaatag ttgttgattt atctaagatt cctggagaaa
     5581 taagcaagat ttcttttact gttacaattc atgatgcaac agaaagaaga caaaattttg
     5641 gacaagtaag taattcttat ataagaatag tagataaaga cacaaatgaa gagttaataa
     5701 agtatgaatt aggagaagat tttagtatag aaacagctat agtagtagct gaaatatata
     5761 agcacaatgg agaatggaag tttaatgcac taggttctgg ctttgaaggt ggtcttgctg
     5821 cattatgtgg aaactttgga ataaacttat aatttcaact ataacaataa actaaagggg
     5881 gatattttcc atggctatag tattaaaaaa aggacaaaaa atcgatttaa ctaagggaaa
     5941 tccaggtctt aaaaatataa aacttggact aggatgggat acaaattcat ttgatagtgg
     6001 ctatgattat gatttagatg taagtgtttt catggttgga gaatctcaaa gagttgaaaa
     6061 agatgaagat tttatattct acaataactt aaaacatcca tctggagcag ttgaacactt
     6121 aggtgataat agaactggtg aaggtgatgg agatgatgag gaaatattag tgaatttaga
     6181 gacaatgcca aagcatatac aaaagatagc tgtaacagtt acaatatatg aggctgctga
     6241 aagaagacaa aattttggac aagtaagcaa ctcatatata agagtattaa attctgaaaa
     6301 tgatgaagaa atattaagat atgacttagg tgaagaattt agtatagaaa ctgcaattac
     6361 agtatgtgag atatataagt ataatggaga atggaagttt agtgcagttg gagcaggatt
     6421 tgaaggtggc ttagaagcac tttgtagaaa ctatggctta aatgtttaat ttaagcaata
     6481 taatatatta aatataaata acctatacaa ataatgtata ggttatatta tataaaattt
     6541 tgtggaggtt tagtatgtcg attaatttat caaaaggtga taaaatagat ttaaaaaaat
     6601 ctaacccagg actgagtaac atacttgtag gtctaggatg ggaccctgtg caacaatctg
     6661 gcggaggttt ttttaaatcg ttatttggtg gaggacaagc tgatatagat tgtgatgcat
     6721 ctgtatttat gcttaatcaa gaaggtaaat taagtggaat aaaagattta atttattttg
     6781 gaaatctaaa aagtgcctgt aaatcagttt tgcatacagg agacaatcta actggtgagg
     6841 gtacaggtga tgatgaacaa atcctagtta atttggataa agttccatca aatattcata
     6901 aattattgtt tgtagtaaat atatacaatt gtgtagatag aaaacaacac tttggaatga
     6961 tagaaaatgc ctatataaga gttgaagacc aaggtaataa gaaagagata gcaaaataca
     7021 atctatctga taattattct gaaaaaacta ctcttatagt aggagctata tatagaaaag
     7081 atggaagttg gcaatttaaa gcaattggtg aaggaacaaa agatgcgggt cttaaagaag
     7141 taatgcaaaa tttagacagg attgagtgtg catatggaat ttaataatga tatcaaatat
     7201 tataatgtag ataaagaaga agttacaaaa gacttatctg ttaatcctga aaaaggttta
     7261 tcagaatcag aagtcaagac tagaagagaa aaatatggtt tgaatgaatt tactccaaaa
     7321 gaagagggta gtttttggga tgatttaaag gaaagtttaa gtgaaccaat gatagttatc
     7381 ttgattattg cagcagtagt aagtgcagta attggggaaa ctcatgatgc aatagggatt
     7441 gtaggtgcta tagcaatagg tatagcaata ggtatgatta cagaaggtcg ttctaagaaa
     7501 gcagcagagg ctctttctaa gttgactgaa aacatagaag taaaagtatt gagagatggg
     7561 gaagtacatc aaatttctaa gagtgaattg gtaccaggtg atatagtata tattgaaact
     7621 ggtgacatgg tacctgctga tggtagactt atagaaagta taaaccttaa aattagagaa
     7681 gatatgttaa caggagaatc tgatgacgta tctaaaaagt gtgatgtagt agtttctatg
     7741 gaagagatag aatctaaagg agcagtagta gtacaggaac caatacctgc aaaacagata
     7801 aatatggtgt ttggtggtac acttgtagct tatggtagag gagctctagt tgtaacttca
     7861 attggagata atagtgaaat gggtaaaatt gctcaaaact tatcagaaac agatgagcaa
     7921 actccacttc agataaaact tggaaattta ggtggtttga tagccaaagt ttctagtgca
     7981 gtagcaggat tactatttat atttatggtt ttccaaatga taagtaagaa tgtattaaat
     8041 gtagatatgt ctggaatatt accgttttta gaatcaatag acccagttaa aacagcattt
     8101 acagtatgtg ttgcgttgat agttgcagca gtgccagaag gtcttccaac tatgataaat
     8161 atgacacttg caataacaat gcaaaaaatg gctaaaataa atgcattggt taccaaaaaa
     8221 gaagcttgtg aaacgatagg ttctgtttct gtaatatgtt cagataaaac tggtacatta
     8281 actgaaaata gaatgactgt agaagttgca tatgttgatg gcagatatat tgaaagttca
     8341 gaagaggaaa taaatagtta ttttgaagaa aactgtatga taaactctac agctgatgta
     8401 gagcacaatg atggtgatat taagtattta ggaagtgcta ctgaatgtgc attgctttta
     8461 tattacaaaa acgtagatta tagacaagtt agaaaaaatt ctaatatagt agctcaaaat
     8521 ccttttactt cagattcaaa gagaatgact tctgttatag gtcaagataa tcatcatgta
     8581 ctattatcaa aaggagcacc agaagtctta cttgaattat gttcacatat tcaaagagga
     8641 aaagacatag ttccattaac tgaaaatata aaacatgaga ttttagaaga aataaagaaa
     8701 ttgcaaataa aatcaatgag aactttaggg tttgcatata aagaaatctc acaagctgaa
     8761 gaagaagcag cagtaactgc tgaaagtgat gctatgaatg taagtgcaat ggaaaacaat
     8821 ttagtattta gtggttttgt tggtataaga gacccactta gaaaagatgt tatagagtct
     8881 gtaaatacag caaataaagc tggtgtttct gtaaaaatgc ttacaggaga taatataaat
     8941 acggcaagag ctataggtga agagttaggc ctattaaaaa ataatatgag agtggttgaa
     9001 gcttcatata tagatacttt agatgatgaa gaattaaaac aagagataca aagtatttca
     9061 atcgtggcaa gaagtaaacc agatagtaaa atgagaatag tttcagctct gcaaaagagc
     9121 ggagatgttg ttgcagtaac aggtgatggt ataaatgatg ctccagcact ttcaaaagca
     9181 gatgttggta tagcaatggg tatatctggt acagaggtat ctaaaaacgc agcagacata
     9241 attttaactg atgatagttt tagtaccatt gtaaaaggta ttaaatgggg tagaggaatt
     9301 tatgataact tccaaagatt cgttcaattc caattaactg taaatgtaat tgcattttta
     9361 gtggctataa tatctcaagt tatgggacaa gagatgccat ttactacaat acaattatta
     9421 tgggtaaaca tcattatgga tggaccacca gcactagcat taggtttaga gccagttagg
     9481 gaccatgtgt taaacagaaa acctgttgat agaaaagcca atataatttc taagtcaatg
     9541 gtgtatacaa tagtattaaa tgcattttat ataacagcaa tactaatgtt acaatctaca
     9601 tttaatttct taggagttga tgaagcgcat aagggaacag taatgtttgc gttatttgct
     9661 tttagtgcac tgtttaatgc atttaactgt agagagttta atgcagatag tatcttccct
     9721 aattttacac ataacaaatt agcactacaa ataatagtgt tgacaggaat tgcacaagta
     9781 atatttactc agctattcca agatttcttt aactctgtag caatggattt tatgacatgg
     9841 gtaaaagttt tatgtgtagc agcaagtgta attgtggtta atgaagttgt aaaatgtata
     9901 ataagactgg taaaaccaaa aaagactgct taaaacaaat tttttagaag aaaacagact
     9961 gactcaaaat atgaattttg ggtcagttct gaataaacat ttaaattggg aggatagtta
    10021 tgggtgactc aaaggtattt gaaaaaatat tctccagcca atcagataga ggaaattaca
    10081 ctccttcaaa gggatatcta agttatttta tttcgtatat tggtttagaa gatgaagttt
    10141 tgtacaatct cgagatattt aaaacaaaac aaaatattga tagcaaaaaa gatattgctt
    10201 tatttactga tgttatagca aatcctagtg attttgatat tataaattat tttaaaagtg
    10261 gacttcaaaa atatagaact agcatggaag atgtagatat aaatattttg ggatttgaag
    10321 agattgatta taaaataaag caagcaatgg atagagtgtt aaaagaagag gaaaaagaat
    10381 ttactaatga tagagttaaa caaaatttta tagtgaaaat aatggcatgg ataaaaattt
    10441 atatcggtgc tttagatata aataaaaatg aagctccaaa agtaatattt tatggagata
    10501 taaaaaaaca tgaagtttat ttactcttaa tattatactt ggcaggtttt gatgttttat
    10561 atcttaatcc aaattcaaag agtaatatag atattttaaa atcagaaaga tacaatatag
    10621 agtttgaaga agccaacata atagaagaaa aaatttcttt tgaagaaagg gttattttag
    10681 gggaaaaaat agataaaagt tctgtaaaaa aagcttttac tgttggggca gaagcatcaa
    10741 agcgtataag tgaggaattg ttaaatgatg caggatttat aaaaccttgg caactccaag
    10801 atagaaaaat aaaaaatcta cttctaagtt caactgttga tgaaatatca atatactgga
    10861 atcaaccact taaattaaga ccaggattta agtttaatga tgctattgta gaagctccaa
    10921 attttctttc taagattaat ggaatatata acgataagaa tgaatatatt aagtttttag
    10981 atttattaag agattcggaa agttcaacat ttattgaatt taatggagat gtagatagat
    11041 tttcaaaagc ttttacaaga gaagctttta gtttatcctt tttattggat tcaaaaggtg
    11101 caatagataa aaactctgtt ttaaataata aagattattc catatcaaca ttagcattga
    11161 atcaacaaat tatgatttta gagaaagtgg aagagttgtt agaaggaagt atgtttttga
    11221 atggtttaag tggagaagac aagataaaag gtctatttac agtacttcac atggataaga
    11281 aatttgttca tatgatgaac aatttcgatt actcacttat aaacccaaaa ttgattatat
    11341 acatgtacaa gagtattgta tttgataaag aaatagtttt tcttatgctt ttacttagta
    11401 agattggatt tgatattatc atattatgtc caggaggaga aaacaatata gagaatgtta
    11461 taaataacca attgattgat gtacacagat tggataaaat ggtatatgac cttaaattaa
    11521 attcgcttga aaatgatata cctttactta aaaaaatttt tggaaagagg agacgatttt
    11581 agatggggat aaatataaat agtttttcaa aagataagaa ctctgtagag gttactgaga
    11641 atttgcctac agcagttcaa gaagaaaact tcgatataat ggaatataca aataataaaa
    11701 aaaatgaact gagaaagtct aaggaagttg aggcattgac atctcttata gaagtagaaa
    11761 atccagatac gattttacag tttggtagga aagcatctga aggagtagca agagtttctg
    11821 atagcctttt aaatacaata aaattaaata gaaatgaaga aaattctaaa atgctggttc
    11881 atttaactaa aataatggac aagtttgatt tagacgattt tcaagaaact aaggaaccaa
    11941 atttcgttca aaaacttttc aaaaaagcta ataatgccat tgaaatgatg tttcaaaagt
    12001 atgaaacatt gggaggagaa gttgagaaaa ttcaaattga gcttgaacaa tacgaaagag
    12061 atatagcact ttctaataag caaataggtg ctatgttaaa tgaaaacttt gagttttaca
    12121 atgaacttca aaaatatatt gttgctggag aaatggcaat agaagaaatg gataatgaaa
    12181 tactaccttt ctttaaacaa aaatcagaga ctagtggaga ccaaatggat gttgtcaatt
    12241 atcaagaact attaaaagtt tatgatatgt taaatcaaag agtctatgat ttaaggatag
    12301 cagaaaatat agctatccaa acaataccta tgcttagagg tatgcaacat aataattatg
    12361 gtttaattag aaaaataaat agtgcatttg tcgtaacttt accagtattt aaacaatgtt
    12421 tatctcaagc tatattactt aaaaagcaag aattacaagc aaaatctcta aaagcacttg
    12481 atgacaaaac taatgaatta ttgcttagaa atgcacaaaa tgtaagtact caaagtgcac
    12541 aaatagctag aatggctgga acaagttctg ttcaaataga aacattggaa aaaatgtaca
    12601 atactattaa atctggtata gatgaaacta tgagaataga agaaaacaat agagctttaa
    12661 ttaaggataa tacaaagaga ttggaagaat taaatacgac tataatttat aataagtagt
    12721 attatattaa gggctaattt gtataaagtt ggccctttct atgtatatga ggaggctaga
    12781 cattgaaata tttcgattat atctgcaaag atgatttgga gagaattttt ttgaaggagc
    12841 cagaagattt tagtgctaag actgaaaaag atgtgttgaa atatgcactg ggcgcttttt
    12901 tatacgttcc agctactcaa tataatatga tttataagag tattataggg gatgttaagg
    12961 gagttagacc attagctatt tgtttagaag atgctgttgg agtaaatgga gagttagagg
    13021 ctatagaaaa tcttagatta attttaaaaa atattagcaa tgaaagtata actaataaag
    13081 atggaatacc actaattttt gtaagaataa aagatgtgga gcaactgttg agaattaaag
    13141 agattataat taaaaataga cattctataa caggaatatt aattcctaaa gcaaattcgg
    13201 agttaataga aaattgtata gaagctttag attctatgaa tcttcaagat atgtatgtta
    13261 ttccgataat agagacaaaa gaatttatat ataatgaaaa aaaagaactt agttttacta
    13321 atttgtacaa tgctatttta agacataagt caagaatatt aagcattaga gtaggcataa
    13381 ctgatatatt agggatgtat ggtataagga gagataagaa cttctctata tataataatt
    13441 tgatttgttc ttcatttata ttagatataa tcacatatct tcaaagacca gagttagata
    13501 tacctataag tggtggagta tcagaatttt ttgatatgac gaataaggat ataagaaata
    13561 agtatataga agaaatatta ttggataaat atcatggatt aattggaaag accgtaattc
    13621 atccaatgca aattcaaatt gtacaagcgc tatctacagt atcatatgaa gattttactg
    13681 atgctttgga tatactggat agtacagaca gtaagtatgg tgtgagcaag ggtgtattag
    13741 gagagagaat gaatgaaaca aatccacatt ttttatgggc taaaaaaaca ttgattctat
    13801 ctaaaatata tggtgtatta aataaaggag tagactatga agaattgctt aaattttaat
    13861 tgtgagattg acattattga taatccattg aatttagaac taaatgattt tttagacgta
    13921 ggaataagaa ataatagcaa aagacgtttt ttgtttatct caaagaaact cggtaaacat
    13981 ttagcatgta agccaagtga gatggataat ttgggaaaat taatggctac aatttataat
    14041 gaaaaaaata aggaagaatc aaatggtact gttatagctt ttgctgaaac tggtacagct
    14101 gttgggcata gtttttttga ttacttatgt ggagattatg aatttattca tactactaga
    14161 gagcagtttg acgagttaga aaatctggaa tttttagagg aacattccca tgcaacagac
    14221 cagaatttat actttggtat gcttaacaac tttaaatttg gagatgagat aatattggtt
    14281 gatgatgaga ttaccacagc caatacatgc atgaatataa ttaggaaaat tcaaagcatt
    14341 tatcctaaaa agaaatacac tatatgctca atactcaatt gggtaagtga tgaaaattta
    14401 agtaaagtta ctcgtttaga aagagagcta gattgtaaga tagattttgt atacttattt
    14461 aaaggaaact ttaaatttta tatagatgaa gatattattt caaattacaa cgataattat
    14521 agtattacta aattagagac tatttcaagt gataaagtaa atttagtagt aaatcatatt
    14581 tatgttgatt tagaagatta tattggaaat aagaaatatg taaaatatac aggaagattt
    14641 ggaattaata gaagagaaca atttagatta aaagaaatcg ttaagagaga aagtaaaaaa
    14701 ataactatgg ccagtaaaga atctttaaaa aatgaaaaag aaaagattct atttttaggt
    14761 actgaagaat ttatgtatat acctatgctg tttgcaaaag aaaaagaaaa tcaatatgat
    14821 atatactatc attcaacaac aagaagtcca attattaata ttgataagaa taattatcct
    14881 attaaaagta aatttatgct aaagagtttt tataatatag atattcaaaa ttatatttac
    14941 aatatagata aacataattt ttctaagtgt tatttatttg tagagctgaa taaaagtgaa
    15001 aattcttatc tagaatttat tgatataatt aaaaaaacaa gtataaaaga attaactata
    15061 gtatgttgta gctaaagtgt tttaacagga taaaatatat cacgatttta agataggagt
    15121 tgagtcatat gagagatttt agcacgtatt ctaaagatga tgttattttt ttattaaaag
    15181 atatatcaaa tttaattgaa gaagaaggaa ataaagaaag agaattaaaa atacaaaatg
    15241 gtagacatta ttcagaaatg ataccaatag agtatgaagt tagtgaagat tatctgaatt
    15301 tatatcatga gaaactaaga gaaaataaag ataaacttgc attttctata ggagttatgt
    15361 gtgaaaaaat aattaaaaaa aatggaaatg atgtagtact agtttcactt gctagagcag
    15421 gaacacctat tggtattttg gcaaaaagat atataagaag caagtataat gtaagtttac
    15481 ctcactatac aatctctata ataagagata agggaataga tatgaatgca attgaatata
    15541 tagtaaagaa tcatccgagc tcaaaaattc aatttttaga tggatggact ggtaaaggaa
    15601 ctatatcaaa agaacttgaa aaagcatgtg atgaacttaa tgttaaattt aaaaaatatt
    15661 ttgactcaac acttgctgta ttagctgacc ctgctggata ttcaggactt tatggaatta
    15721 gagaagattt cttaatacca agtgcatgtt taaattcaac agtttctggt ttagtaagta
    15781 gaactgtact aagagaggat ttaattagta aaaatgactt tcatggagct aagttttata
    15841 gacatttaaa agataaagat gaatctatga actatatcga aactatagaa gaatgtttta
    15901 aaaatcaatt taagaatata tctgatgaag ttgaaaactg ggaaagtgac attataacaa
    15961 gagatggata ctttgatgtt ttaaatataa aagaaaagta taacataaca gatattaact
    16021 ttatcaaacc aggtgtaggg gaaacgacta gggttttact tagaagagtt ccgtataaga
    16081 ttcttgttaa agacttaaat gataaatctt tagaccatat attcatatta gctaaggaga
    16141 aaaatgttga agttgaacaa atggatttaa aagcctataa atgttgtgga ataataaaaa
    16201 atatgaaaga tatttaggaa atatattaga caggagaata ttgaatgatt gttttttcag
    16261 atttagacag gagtatcatt tactccaata aatttttaaa tgctgatagt aaatatgcta
    16321 atatagaaat ttatagggaa aaagaaatat cttatatatc tttagatact ataaatttaa
    16381 taaagcaaat acaatattat ggtatgttta tacctacaac tactagaaca gtggagcaat
    16441 ttaagcgtat agaatttaat aaatatggga tttattttcc ttggagtata acttcaaatg
    16501 gaggagttat acttaaagat aatgaaatat tgaaatcatg gagtgaaaaa atagataaac
    16561 taaaatctga ttatgagccc attgaaagta tgattcataa atttaaagac tatttgaatg
    16621 tagatggaat aactaatttt aaagttgcag aagatacatt tttttatata gttgttgatt
    16681 tatcaagatt taatttggat tcaatcaaag aatatacaaa tatattggaa tcaaaaaatt
    16741 ggaagtttta tgtgagtggc agaaaaatat attttatacc aaaagaaatt tcaaaagaaa
    16801 atgctataaa atacttgact aaagaattag gaatagaata tttttatgca gttggagatt
    16861 ctattatgga ctatggtatg cttaatatct caaataaatc ctatgtattg aagcatggtg
    16921 atataaataa aaatgaaata gaaaattcat ttatatcaag ttctttcaat ggaatgagtg
    16981 gaactgaaga gatactatct aacattttag acgagaactg cctacttaat atataatgac
    17041 tgatatataa ataataaact agaaaaatct gaaataaatt atagtaaagt ttatcatact
    17101 aatttatttc agatttttat caatttatat ttaagtaaga actcaatatg ttaaaatgaa
    17161 ggtggagtca ttgcccagac tgagatacat acctcatcat cataattttc atacacatgt
    17221 ggtatagtac tgttaaaata aaaactatct ccttcctcca atagatattc cttatttcca
    17281 agtgttatct tcattttacc ttttattata tagccacatt cttctccttc gtgtgtcaca
    17341 aattcactgt ttttgtgtgt atgaccatct agtgtaacaa gtataaattc tatttttttc
    17401 ccagataaat taggaacaag aagttcgtaa aattttgtag aatcattagt tactattttt
    17461 tttcgattat ttttctttac tactatatca gagtcttctc ttccaatctc ttcaaaaaaa
    17521 taacctatat ttatatcaag agcttttgat attttccaca ttgctacaat agatggaaca
    17581 accatattac gctcaatttg acttattaag cctgtactta aatctgaatt ttttgctaaa
    17641 gtagagatac ttatattttt agattttcta agagatctaa ttttgcttcc aacatctata
    17701 ttcattagat acaaaccccc tcagacatga ttataacata taatttataa agattaataa
    17761 aataaaaaaa aagttttaca atacaagtat tttttatgct atattgaatt ttctgaaaag
    17821 atgcattata atagtattaa tattcaatat attgaatatt gataacgata ttttaattta
    17881 atgaaatgga ggtaattaag atgaagagtt ttgtaagtcc tgataatatt tgggtattat
    17941 gggcagttgt aactggatgg gcagcattta gtatttattt ggaacaaaag tacaattggg
    18001 catcaaaagt gtcgggagca ataatagctc ttgtaggagc aatgttatta tcaaatctaa
    18061 atattatacc agtagagtct gttgtatatg accaagtatg gggatatgtt gttcctttag
    18121 ccatagcttt attgttatat caatgtaaca ttaaaaagat atggaaagaa agtggaagat
    18181 tgctcattat atttcttgta ggttctgtag gaactgtact tggagctatg attggatttt
    18241 tagcattaaa aaatgtagta ccagatttaa atattgttgc agctatgatg acaggttctt
    18301 atataggtgg aaatgtaaac tttgctgcta tgtcaggggc gtttgatgcg ccaggagaat
    18361 tagtatcagc aacagttgta gcagataatc ttttaatggc attatatttc tttgtcttaa
    18421 tagctatacc atcaattgga ttttttagaa aacattttaa acatccacat gtagatgaaa
    18481 tggaaagtat aggtataact gaaggagaaa cagtagcagc aaattattgg ggaagaaaag
    18541 aaatatcttt aaaagatata gcaatagcca ttggtagcgc ttttataatt gtcgcagtat
    18601 ctgtagaact atcaacttgg tttaaagcta taataccatt atcaaatcct tttttagcaa
    18661 tgttaaatac tttatttgga aatcaatatc taataataac tactttgact atgttgtttg
    18721 ccacatttat gcctaatttc tttggtgata taaaaggagc acaggaattg ggtacatttt
    18781 taatatatat attctttgtt gtagtaggtg ttcctgcttc aataacatta ataattcaaa
    18841 aatcaccatt actactttta tactgcggaa taatggtact aataaacatg ctagttacat
    18901 ttatagttgc aaaaatattt aagtttagtt tagaagaagc tattcttgca tctaatgcaa
    18961 atattggtgg tcctacaaca gcagcagcta tggcaatatc aaaaggatgg tctaagctag
    19021 tagggccaat acttatagtt ggaacatttg gatacatagt cggtaattat tttggtcttt
    19081 tagtaggaaa tattttgata taattttatg tgaaaaaaag ttgtttttta gaaagatttt
    19141 tatttaattg gaggttgtta tgattttcga tggacattca gatacttggg cagatgtaac
    19201 aataagaagg cttaacgggg aaaaagatgt atttagaaga atccatcttg ataattttaa
    19261 aagagcaaat atagaaggag ctatttttgt aatatggata gacccaccat atgataaaga
    19321 gcctgaaaaa agagttaaac aaattataag ctctataaaa gatgaaattg aagatatgaa
    19381 agatgttgta aacatagtaa aaaaatattc agatatagat aaaggtataa gtgaaaacaa
    19441 aataaatatt ttaattggtc ttgaaggatt aagccatatt ggaagaaata tagatttact
    19501 agattatttt tataaagaag ttggtgcaag acatgcatca ttaacttgga atgaacagaa
    19561 tgaacttgct acaggatgtt taggggatga aaatcgtggt ttaactgaaa taggtaaaaa
    19621 agcagtcaat aaaatggaaa aattgggcat gatagtagat gtgtcacatt taaatgaaaa
    19681 atcattttgg gatttaatgt ctgttgcaag taagccagtt atagcttctc attctaatgc
    19741 taggagttta tgtgatgcta aaagaaatct tacagataat caattaaaag aaatttcaaa
    19801 tacaggtgga ttagttggaa taaatagttt tagaggattt actaatagta atcctaagaa
    19861 gcaagattta atacatttgg ttgaccatat agactatatg gtaaatctta taggtataga
    19921 acatgtatct tttggttttg attttagtga ttacttaggg aaagagacta taggttcttt
    19981 tactggaaat gctagtgtgc caagtgtaga tggtcttgaa agcacacata cagcacagaa
    20041 catagtagta gagcttgaaa atagaggtta taaaaagaaa gatatagagt taatctctag
    20101 tggtaatttt tataggctct ttaaagatat actaaaataa tataataaag aacttaaatt
    20161 tataaaatag tatatttata caaataatat ctacatattt aaattggcgt tcataattta
    20221 ttaaattaaa ttttaatgtt actagaagtt tcaattctag taacattttt tatgttaaaa
    20281 acagatatag atatatattg acaatgataa ttattattga taaactataa aagtgaaaat
    20341 gataataatt ttcattttaa ttaataaaag atttttaagg agggagaatg aagaaaaggt
    20401 atttagtcat aggaataata attttgtctt atttttcttt atttatagga gctgaagata
    20461 taaatataat gcatatattt gccaaaaatc agcataaact tatgatattt atcatgagta
    20521 gaataccaag attaataagt atattgattg ctggtgtagg aatgagcata gcaggtctta
    20581 taatgcaaca gataagtaag aataaatttg tatcacctac aacaggtgct acaatagatg
    20641 cagcacaatt tgggatagta atatgtatgc ttttagtacc aactgcctct atttttacaa
    20701 agacaataat agcatttgta ttttctcttg tagggacttt tacgtttatg aaaataatag
    20761 ggaagttaca atttaaaaat ataatctttg taccattagt aggtataatg tttggaaata
    20821 taataggttc tatgacggat tttatcgcgt ataaatacga tttgagtcaa aatgttagtt
    20881 cttggatgca aggtgacttt tctatgatac ttaaaggaaa ttatgagata ttatatatca
    20941 caatacccct tataatatta gcatatattt atgcaaataa atttactgtt gttggtatgg
    21001 gtatggattt tgcgacaaac ttaggtctta gttacaaaag aattgtcaat ataggtctga
    21061 taatagtagc acttgttaca gtatgtgtag tcgttacagc tggtaatatc ccttttatag
    21121 gtctaatagt tcccaatatc gtatccttgt atatgggtga taatataaga gcaagcatat
    21181 ggtatactgg tttacttgga gcgatatttg tacttatatg tgatattttt ggaagaatta
    21241 ttatttatcc atatgaaata tctatagggc ttacagttgg agttatagga agtatactat
    21301 tcctttattt aatattaagg aggaatgtaa atgaagctta gtctcaatca aaaaatttac
    21361 tttgtaagta tattactatt catatcaata gcacttttta taatttatgg aatagacatg
    21421 aatcatttag aatatgcatt atcacaaaga ataccaaaaa tatttgctat ggtattaggt
    21481 ggaggatgta tagcatttac tactgtagtg tttcaaacaa ttacaaacaa tcaaatatta
    21541 acaccaagtg tactaggttt agattcattg tatgtaatga ttcaaactat aatagtattc
    21601 atatttggtt caagcagtag tctgattata aatgaaaatt ataattttat tattaatgta
    21661 gctatgatga ttggagcttc attgttatta tataagacgt tatttgaaaa aaataaaaat
    21721 aatattttct ttttaatcct agtaggaatg atatttggaa ccttatttaa aagtgcaaca
    21781 acatttattc aggtaatgat agaccctaat gaatttttag ctttacaaac tagtataatg
    21841 gcaagtttaa acaatataaa cactaatgta ttgttaatag catttataat aattattgcc
    21901 ataatacctt ttatatatga tgaaataaag tatttagatg tattgtcgtt aggtaaagaa
    21961 caagcaatta atttaggtgt agattttgat aaagcagtta aaaaaatgat aatattaata
    22021 gcaattttag tctcaatatc aacagcactt attggaccta tgacattttt aggactttta
    22081 cttgctaata taacaagaga aatttttaaa acatataagc atacatactt gatttcgggt
    22141 tctatgttga tagggatgat tacattgata ataggtcaat tttttataca acatgtattt
    22201 aagtttgata caactttaag tgtagtaata aattttatag gaggaattta ctttattcaa
    22261 ttactattaa agggggcaaa tagatgatag agataaaaaa tatatttaaa agatataaga
    22321 ataaaaatgt tgtagatgat gtctctttta gtatagaaaa gggaaagata acatctttta
    22381 taggaccaaa tggagctgga aaaagtacag tactatcaat agttacaaga cttataggag
    22441 gtgatggtgg agaagtaata atagaaggaa aaagcttgac taattatagc aataaagaat
    22501 tagcaaaaaa aatagctatt cttaaacaat ctaacaatat aactttaaag cttacaataa
    22561 gagaattagt tggatttgga agatttccat atagtgaagg taatctaact aaagaggatg
    22621 aaaattatat tgatgaagca atagaatata tgaagttgac tgatatacaa cataagtatt
    22681 tagatgaatt gagtggaggt caaagacaaa gagcttatat agcaatggta atagctcaag
    22741 atacagagta catattactt gatgaaccac tgaacaattt ggatatgaac cattccgtac
    22801 agatgatgaa ggtacttaga agtctttgtg atgagttaga taagactata gtattagtaa
    22861 tgcatgatat aaattttgca tcatgttatt cagataatat agttgcatta aaaaatggaa
    22921 aagttaaaaa agtaggtcgt acagatgaga tagtaaatga aaaagtttta gaagatattt
    22981 atgagatgaa ttttaacatt aagaatataa atggaaatcg aatctgtatt tatttttaaa
    23041 atattgtata tgaaaataaa cttgctatat gattttttat aaagttttta aattaaaata
    23101 aagattaata ggagagagga aaatattgat atgaataaaa aagcagcaat agtagcagca
    23161 gtagctatta taggattagt gacagtgttt gcattaggtg gaagtaaaaa gaatgaatca
    23221 aaaacatcag aaaattcaaa taatactatg aagataactc ataacttggg agaaacagat
    23281 gtaaaattaa atcctaaaaa agtagtggta tttgattatt cggctttaga tacaatggat
    23341 gcattaggtg tagctgagaa tttagtagga cttccaaaag cgagtttacc agcatcctta
    23401 gaaaaatata aggatgacaa gtatgcagat ttaggaggat taaaagaacc agatttagag
    23461 ggtataaagt cggcaaatcc agatttaatt ataataaatg gaagacaaga agatttttat
    23521 gagcaattgt caaaaatagc accaacaata agtacaagta aagatgataa gaagtactta
    23581 gaatctgtta aaaataatat agacaaaata gcaaaaatat ttggagtaga agaaaaagca
    23641 aatcaagaat ttagtaaaat tgaaaagaaa atagaaactt tgaataaaaa agtaacagat
    23701 aaaaatttaa atgctttaac tataatggta aatgaaggta atttaagtgt atttggtgaa
    23761 gaatcaagat ttagtatatt atacaatagt tttggatttg aaaataaaga taaaaatata
    23821 aaagaatcaa gtcatggaca aaatattact tttgaatata tagctaaaca aaatccagaa
    23881 gttatgttcg ttatagatag aggaatagct actggcagtg atgtaaaaga aagctcaact
    23941 gcaaaatctg ttttgaacaa tgatataata aaatctatgg atgcatataa gaatgataat
    24001 ataatatatt tagattcacc aacatggtat gtaaatgatg gtggtctaac atctttaaat
    24061 aaaatgatag atgatgcttc aaaagcggta aattaataat ccgatataat caaaatatat
    24121 tttttataaa atgattatca aataaactag gagtttttaa gaactcctag tttatttttt
    24181 gcataaaaaa gatatagttt aaacaataat attatctata ataagataaa tctattcaaa
    24241 atctaataaa atatgaagtt at
//
LOCUS       DAMJWU010000048        24074 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMJWU010000048 DAMJWU010000000
VERSION     DAMJWU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.048"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            43..894
                     /locus_tag="PC460_002146"
     CDS             43..894
                     /locus_tag="PC460_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF2601030.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNGLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1027..2640)
                     /gene="ggt"
                     /locus_tag="PC460_002147"
     CDS             complement(1027..2640)
                     /gene="ggt"
                     /locus_tag="PC460_002147"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF2601031.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2865..4802)
                     /locus_tag="PC460_002148"
     CDS             complement(2865..4802)
                     /locus_tag="PC460_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601032.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKIIELIDKDINIEMYYKNRNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDANEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYDNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4789..5556)
                     /locus_tag="PC460_002149"
     CDS             complement(4789..5556)
                     /locus_tag="PC460_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601033.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5844..6518
                     /locus_tag="PC460_002150"
     CDS             5844..6518
                     /locus_tag="PC460_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2601034.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6542..7546
                     /locus_tag="PC460_002151"
     CDS             6542..7546
                     /locus_tag="PC460_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2601035.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7658..8887
                     /locus_tag="PC460_002152"
     CDS             7658..8887
                     /locus_tag="PC460_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2601036.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8895..9569
                     /locus_tag="PC460_002153"
     CDS             8895..9569
                     /locus_tag="PC460_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601037.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9572..10804
                     /locus_tag="PC460_002154"
     CDS             9572..10804
                     /locus_tag="PC460_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601038.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11090..12355)
                     /locus_tag="PC460_002155"
     CDS             complement(11090..12355)
                     /locus_tag="PC460_002155"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF2601039.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12408..13556)
                     /locus_tag="PC460_002156"
     CDS             complement(12408..13556)
                     /locus_tag="PC460_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2601040.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNKVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13656..14201)
                     /locus_tag="PC460_002157"
     CDS             complement(13656..14201)
                     /locus_tag="PC460_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2601041.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14424..15368)
                     /locus_tag="PC460_002158"
     CDS             complement(14424..15368)
                     /locus_tag="PC460_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2601042.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            15754..16635
                     /locus_tag="PC460_002159"
     CDS             15754..16635
                     /locus_tag="PC460_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2601043.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(16696..17904)
                     /locus_tag="PC460_002160"
     CDS             complement(16696..17904)
                     /locus_tag="PC460_002160"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF2601044.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(17977..18229)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(18381..19370)
                     /locus_tag="PC460_002161"
     CDS             complement(18381..19370)
                     /locus_tag="PC460_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF2601045.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            19522..19962
                     /locus_tag="PC460_002162"
     CDS             19522..19962
                     /locus_tag="PC460_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2601046.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            20289..20516
                     /locus_tag="PC460_002163"
     CDS             20289..20516
                     /locus_tag="PC460_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2601047.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            20542..22662
                     /gene="feoB"
                     /locus_tag="PC460_002164"
     CDS             20542..22662
                     /gene="feoB"
                     /locus_tag="PC460_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2601048.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(22795..23613)
                     /locus_tag="PC460_002165"
     CDS             complement(22795..23613)
                     /locus_tag="PC460_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2601049.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            23820..>24074
                     /locus_tag="PC460_002166"
     CDS             23820..>24074
                     /locus_tag="PC460_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601050.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIA"
BASE COUNT         8460 a         3644 c         3054 g         8916 t
ORIGIN      
        1 taaattaaaa taaagataat ataaacacag gaggtacatt ctttgaaaaa cattatttca
       61 gcaaaagaac ttatatcaaa actagaaaaa aatgataatc tagttgtaat agattgcaga
      121 tttgatctta tcaatagaac ttatggtatt gattcttata aaaaaggaca catcaaaggc
      181 agctttatct tagatataga caaggattta tcatctccta caaaagaaca tggtggtaaa
      241 aatcctttac aagacccttt aatactaaaa gaaaaattag aaaaaatggg tgttgataac
      301 gacactacga ttgtaactta tgatgatgga gatttaaatg gagcttgcag attatttttc
      361 caattaaaac atttggggct taaaaatgtc tatgtactgg atgggggaat aacatctttc
      421 gtaaaggaag gtggagaatt ggaagagaaa gtgaatatac caagttgtac aggtaaggaa
      481 attagaccaa atataaacaa tggtttagtt gttccaatgg aatatgttaa gtctaaatta
      541 tacaaaaaag atactgtaat aatagattgc cgtgctaatg agagatacca gggtttagtt
      601 gaacctgctt actcaaaagc tggtcatata ccaagtgcaa aaaattattt ttgtaaggat
      661 ttaataaaaa gtgactttga aaatggctca cttaaagata tagaattttt aaaaaaattc
      721 tttaaagatt taaataatta tgatgaagta attttatctt gtggttcagg gatatctgct
      781 tgtgttaaca gtcttgctct tagagaactg gatatacctc acaagattta tattggtagc
      841 tttagtgact ggattagtta tgatgacaat gaaatcaaaa ctggtcaaga ataaggctca
      901 taatctatat aaaatcagca tatgatagct taaattttgt aaaggcgtca gtcattgtag
      961 actttcgcct ttactaatta tttcatatac tctttaaatt tattatatat attttagttg
     1021 ataatattaa taaactgcaa tatgaccatc acatcttgat tccgtcccac aaatataact
     1081 ttcctgttct gttttaaata ttatttgacc tcgtcccatg tcgacagtat catgaattac
     1141 ttttatttca tgaccctttt caattaatga atctattatg tgctttggca tctctggttc
     1201 aacttctatt tcctttcctt ttatccactg ccatcttgga gcatctagcg cctcttgtgg
     1261 atttaatcca aaatcaatca tatttgttaa cacttgaata tgtccttgtg gttgcataaa
     1321 agctcccatt acaccaaatg gacctattgc tttatcttct ttacctaaaa atccaggtat
     1381 tatagtatga taaggttttt taaatggttt tactacattg tggtgtttag ggtctaaatt
     1441 aaaattattt cctctactat gtaaagcaat cccagttctt ggtatcacaa ttcctgaacc
     1501 aaaatttatg taattgctct gtatatatga aaccatattt ccatccttat ctgcagtaca
     1561 aaggtatact gtaccaccac aatatggctc tcctgcagtt ggatacaaag cctctttatt
     1621 gtcaatcaat aaacttcttt ttttagcata ctcttggctt agtaattcct gaatctttac
     1681 cttcatatgc tctatatcag ttacatatgt tttagaatct gcaaaagcta attttaaact
     1741 ctctataatt ttatgtatgt cttctacatt ttctatattt ccttctaatt ccaattcttt
     1801 taatatgtta agtgccatta aaactgttat tccatgacca tttggtggga tttcaaatat
     1861 tttatatccc ttatattctg tacttatagg ttctacccat gttggataaa aacactctaa
     1921 gtcactcttt cttatagccc cattaaattt tttagaatat tcatctattt tatcagcaag
     1981 tcttcctcta taaaagcttt cagcctgagt atttgctatc tcttctaatg tacttgcctg
     2041 ttcttcacat ataaatatat ctccagcctc tggagcttta ccatctttag aaaatgtatc
     2101 aaaccatggt ttgaactctt ctccaataag ttctttttca tagagttcat atgatttttt
     2161 ccatacctta gctacattag gagatactac gtatccctct cttgcatagt ttatcgctgg
     2221 agataagcat tccattaagc taagtttacc atatttttta tttaattctg accaagctgc
     2281 tggtattcca ggtacagtta ctgctccaaa cccatatttt ggcatttctt tcatattatt
     2341 ataatttttt aattccatat tttctggagc aaagccacta gaatttaaac catacaatcg
     2401 tttttcttcc tctatccaaa caagggcata tgcatctcct ccaatcccat tgcttgtagg
     2461 ttctactact gctaatgtgg cggctgttgc aactgctgca tcaacagcat ttccaccctt
     2521 ttttagtatt tcaagtccag cttgagatgc taatggtgtt gatgttgcaa ctgctccatt
     2581 tttagcatac acgacatttc ttattgatga gtatttatgt tggtaggcat taaacttcat
     2641 aaatttcagc tcctcgtcat tttttatttg atattctgct tagatttatt atataacatc
     2701 aattaatgta actgatacaa aatgatataa aatatagact ttttcctaac tttaatttta
     2761 tctttaaata aatatcttaa actcaattct agtattttct atctgcactt aaataagtta
     2821 ttttattcaa aacatatatc taattctttt aacttaaaaa taatttattc ttttatcatg
     2881 tttagagcat attttaaagt taaaaaataa tatataaaat atcctataaa ataaatgact
     2941 agagttatga taattggtac aagtaatgac cttggtataa acttttgtgc tatagacata
     3001 gcaaataaat tatgaactgt acctacaact aatggcatta aaaatataac ttttaactgt
     3061 tttgatatta ttttctcaat atctttatta tttgctccaa gttttattaa cataatatat
     3121 ctttccttat catcatatat atttgataat tgtttaaata agattatact acctgtacat
     3181 aaaagaaata ctacagacaa aaatatgcct ataaacagca ttagaccact taattttatc
     3241 aagttatcat aactttcaat acttgatgta aaattaaaag gataagtaaa tttcatattt
     3301 tcacttacta tattttttaa ttttaaagtc aaatccagtt cttttttttc attttttata
     3361 tcaattaatc ttattaatct agaatttcca tgtaatttta actgattaaa tacgtcatct
     3421 tttactacaa ttaaatcaaa cgtagcttgt gaatttattt ctggctccaa gatgcttttt
     3481 tgtactttaa acagatttat atcatactca tttgcatctt taatatgcaa gtttattttt
     3541 tcaatctcaa agtctttaaa aaatattttt ttgtatgaat cagaaacata gtacacagat
     3601 tcctttgaag ttaattcttc ataattgtta ttctgatatt gtcttaattt tttaaaatca
     3661 ctttctctaa taatatctac agacgttcta tcttctatat aactatttcg atttttataa
     3721 tacatttcaa tatttatatc tttgtctata agttctatta ttttatcaaa gattatctta
     3781 ttatcttttt tatatttata taataatttg tcaatttcgt tatttacata agaatttcct
     3841 aaatttatgg tataactgta tttataattt tcacttatgt tcctatctat atcgtaataa
     3901 agtgatatgg taaaccctag catagttaca cttgtagcta ttaatattgc tattacagcc
     3961 aatacactac tattgctctt taatttaaat ccaagttcag aaaatgcaat taaattctgt
     4021 cccttataat aaaaactttt tctcttctta acagcatcaa taatcatata catagttgat
     4081 gaaaaaaata aaaaagttcc aggtattatt gtaactaata taaaaactga aagcataata
     4141 ttttcagcaa taaaataact aattgacatt aaataagaaa tagctattaa taaaacacca
     4201 agtacacatt ttaactttat aaatttttta aacttatact tttctggata tttattaaat
     4261 aactctataa ttctcttatt tctgataact atagtatttc ttattccaat aactatgaat
     4321 attattaaaa ataccgctag tgtctgaact aaggccttta tacttaagtt catatttact
     4381 actatcattt cattcattaa ttttacaagc atcatagtca taagctttga aaataacagt
     4441 cctaaaaata tacctattag tattgcaact atacccatca aaaaagtttc atataaaaat
     4501 atttttccta tctgattttt cctcattcca agtaaactat aagttcctac ttctcgccta
     4561 cgtctattga aaaaaaatga gtttgaataa tatataaata aaaatacaaa taccactatt
     4621 acaattgaac ttgtattaat actcatccta gttttctctc caagtgaact caacgcttca
     4681 ttatactcta tagatttaaa actaaaaaat acaaatacac taaatacaat tgaaataaag
     4741 tacattaaat aattaaaaaa actattcttt acattttgca ttgcaattct aaataaagtc
     4801 atttttatta actcctccaa ttaaggaaac tgtatttact attttattat agaactctct
     4861 gttactttct ccctttacaa tttctgtgaa tattattcca tcctttataa agagtattct
     4921 attgcaaaaa ctcgcactta tagcatcatg agttaccatc aatattgttt tattgctgtc
     4981 tttgtttaat tttaatagac aattcaacaa atccctagca gatttagaat ctaatgctcc
     5041 tgtaggctca tctgcaagta ctaatgatgg ttttgttgct atagctctac atacagctcc
     5101 tctttgcttt tgtccaccag aaacttcgcc agggtattta tctaatatag attctatatt
     5161 taattctttt gaaatctctt ttattctatt ttctattact gatactcttt cattttttaa
     5221 agaaagaggc aacattatat tttcttttat tgtaagtgta tccaaaagat tagaatcttg
     5281 aaatacaaag cctagattat cacgtctaaa atctgccaat tcattttgct tcattttaat
     5341 agtattcttt ccatttatat aaatatctcc tgatgaaggt aaatctattg tagaaataat
     5401 atttaaaagt gttgatttac cagcaccgga agaccccata ataccaacaa attctccttt
     5461 ataaaccttg aaagttattc ctcttagagc ttcatgtttt gaaccttttc ttccatactc
     5521 cttttttata ttttcaacag ataatatttc ttccatagtt aataatcacc tttcatcaca
     5581 attagttatt tatcaattct cacaaatata ttatatatat taaaatggct tttaaacatt
     5641 taattatatt aatttaaggt tacattttcg taagtattaa ttatatttct tcaaatttat
     5701 tgcaattatg tttaaaatat aatttatcaa gtaaaatata gttatattaa tataattatt
     5761 ttaggtaata aggtagaaat attttaattt atcaagtaga atatagttat gttaatataa
     5821 ctattttgaa ttaggaggta agaatgttta aaataatggt agttgaagat gacgtctcat
     5881 tgaaaaatat catagcaaag tgtcttacta aatggggaca tgatgttcat caaatagaaa
     5941 atcttgaaaa tataatagaa gagttcaaaa actataaccc agaattagtt ttattggaca
     6001 taaaccttcc attttatgat ggttttcatt ggtgtaatga aataagaaaa atttcaaaag
     6061 ttcctataat ttttatttct tcaagaaatt ctaatatgga tgtaataatg ggtgtaaatt
     6121 taggtgcaga tgattatata caaaaacctt tttctgtaga tgtcttagta gctaaagtta
     6181 atgccctttt aagaagaact tataattttg ttgataataa ctcaaatcag attattcaca
     6241 atggtgttac tttagattta tcaactgcaa caataaacta tgaagataat actattgagc
     6301 taacaaaaaa tgaaatcaaa attctccatg agcttatgaa atataagggg cagatagtaa
     6361 gtaggaataa actcatgaaa aaattatggg ataatgattg gtttgtagat gataatacat
     6421 taactgtcaa tgtaaataga atacgctcaa aattaaatga aattggttta gaagatttta
     6481 tagaaacaaa aagaggattg ggttatataa tcagttaaaa caattctaag taggtgaatt
     6541 tatgagcttt aaagattttc tcaaggataa actaggtttt ttagtataca attttacttt
     6601 cttaattttt acagtatcag ttattatatt ttctcctgta gatgtttttc tgactgatac
     6661 tattttatat atattagttg taaatgttgt gtttttattt ttatatctat ttattagcta
     6721 catcaagaag aacaaatttt tagataagat aaaaaatgat acattcaaaa ttaatatgaa
     6781 tgatatagaa ttagctagat gtaaaaatga agaaaaaata tacttgaaca ttattaaaaa
     6841 tcatcaatat caatgtaata acataataaa taataatatc gagaaatttg aagaaaacaa
     6901 agagatttta aatatgtggg tacatgatgt aaagatacct atagctataa taaaactcat
     6961 tcttgaacaa aatgaaaatt taattgatga aaaaatttca gatgacatag atagtgaaat
     7021 atttagaatt gaaaactccg ttgaaaaaat tttatattta tcaagactcg aagattttca
     7081 taaggatttt ttaattcaag aggtaaatat tgaaaaaatc attagaaata ttataaaaaa
     7141 atactcaaaa tattttattt caaaaaaaat tattttagac ttaaataatt tgaattttaa
     7201 ggttttatct gatgaaaaat ggctatactt catattcgag caattattga gtaattccct
     7261 aaaatactcc tcattaggag ataatatatc tatctattgc aaaagaacca aaaattattt
     7321 acaattaaga gtggaagata ctggttgtgg tataaaaaag gaagatttaa atcgaatatt
     7381 caataaggga tttacaggta ataatggaag aaacaattct aaatctacag gtcttgggct
     7441 ttatttagta aaagaacttt gtgataagct tggtcataaa attgatgtag attcagagta
     7501 caaccaatat acaaaattta ctattacatt caaatgtgat ttatagtatc ttattattga
     7561 tagtttattt tcattaactt atattcaact tcttatagta attctatatg tacatcccag
     7621 tatataatta agtagttttt ataaaaagga ggaagacatg tcttttctaa aaaaatttaa
     7681 aaataaaaga tttagaaata acagttttaa aagtaacaac tttagaaata gcagttttgg
     7741 aagtagtggc ttcaagtcta aaaataaagg ttttaaaaac aatcccttta aaaataagag
     7801 attcaaaata tatgcaggaa tagcagtaat catactttta attctaggag ttcttgctta
     7861 tgttgattcg aaaaacactg aattacaagc taatgaaaat tttatcgaaa catatactat
     7921 tcctgaaaat gaaaaaatat ttataaatgg gatggttgtt cctaagcaaa ctaaggactt
     7981 caatatatct ggagattatg agttaagtga tgttaatgtt acaaatggtc aaaaagtaaa
     8041 tcaaggagac cttcttttta ctgctaaaaa tccaactata atagcagaaa tagatagttt
     8101 aaaatcacaa cttagtcaat acaaaaaaca aaaaatatca ctttctgata ttgctgaaaa
     8161 taaagatgct attgcttcta taaatgcaca gataactgct ttaaactctc agatagcttc
     8221 tctggataaa aaagcttatg acagagttac tgctcctttt gatggcacag tatacttgaa
     8281 tgaccaaact ggaaatccag accaaccagt ttcatttatg actattcaag gtctagagtt
     8341 ttatatgaaa ggacaagcaa gtgaacaaga tttacctaaa atgaagattg accaaatggt
     8401 caatatacta gttttttcaa ctgaccaaaa gcttactggt agaataagtt ttatatctga
     8461 taaaccatct actccaaata ctgaaatggg agctcagcag aatacactat cttattatga
     8521 tataaacata gcatttgata atcaagaagg attagtaaat ggattccatc ttcaagcatc
     8581 acttgaagta gctaattcaa gctttaaaat accagcatct tgtgtactta aagacaacaa
     8641 acactcatat gtatttaagg atttagatgg tatattgaaa aagcaaattg tagatgtagc
     8701 aagtcaaaat gatgattttg ctgtagttag aggcggtctt gaacaaggcg atataataat
     8761 aaaacaccct actaaagaaa tgaaagaagg agacccagtt caaggcgatg gcgtaactgc
     8821 tggttctaat aatggagata ctactccaaa caaaaaagta gaagaagtta ctatggatgt
     8881 gaattaattt acatatgtta attaaattag aaaatattca aaaatattac aaagtaggga
     8941 aagatgaatt acatgtttta aaatccttaa atttagaaat tgaatctgga gaatttgtca
     9001 tgataatggg taagtctggt agtggtaaaa ctactttact taatatctta ggttttctag
     9061 atgtatttga tgaaggaaga tatatatttg atggaacaga tgtaactaat ttgagtgaaa
     9121 atgaacgttc tgtttttaga aatattaata ttggatttgt tttccaacaa tttaatttaa
     9181 tagaaactct aaatgtatat caaaatgtag agttgcctct aatttacaat aaagcactaa
     9241 aaaaatctaa tagagaagaa attgttaaag ataaattaag ttctgttggg cttttggaca
     9301 agcttaagca gaaaccactt caattatctg gtggtcaaca acagcgtgta gccattgcac
     9361 gttgcctagc aaatgaccct cagataatct ttgctgacga accgactggg gcattagata
     9421 gtgaaactag cagagaaatt atggagcttt taacaaggct caacaaacaa ggtaagacta
     9481 taataatggt tacacatgat caagatttaa ctaagtatgc aactaaggtt atacgactta
     9541 aagatggtgt gtttacttcg gaggtgtaat catgagttta ttaaaaaatt ctttggccaa
     9601 tttaaaaggt cacaagcttc gtgtatttgt agctttactc tggattataa taggaataac
     9661 ttcagtaata cttgttagtt ctataggtaa tggatttcaa aaagaaatta agaaatctgt
     9721 aaacaatgta aaccctaata aaactactat ttcgtttgaa tcagccgata acacaggtct
     9781 tactgatgat atgagtattt ttttaaaacc ctttaatgca aaagatttag aagaattatc
     9841 atttgttgaa ggagtagaaa gaatagctcc ttcaagagat gggtttaacc ttgattcagt
     9901 ttactcttca caagcatctt ttgataaaaa aactacgtat gttgatgttg gaccagtaaa
     9961 aaaagattct aaaataaatt taatttgtgg tagagatttt tctttagatg atgaaaaaag
    10021 aaaagtaatt ttacttacat tacaaagtac aagtgaaatt tttgaaaatc cagaagatgc
    10081 cttaggtcat ggtatcaaca taaatggaac tatatttgaa ataatagggg tattagatga
    10141 ttctcagcaa aaccaagctg gaggattctt tggaggttac caagatatgc aatttactac
    10201 ttccctagta cctaaaaagg cttttgacac tttaatgagt caaaattctt attcaaatga
    10261 aatttaccag ttagacttag tttcttctaa aggctataat gtaaatgaag ttgcaaacaa
    10321 tgttatagct aaattatatg agatgcaccc tggtatcaat ggttcttata ctacaccaga
    10381 cccaactgaa cagacagctt atcttgaaag cattaattcc aatgtaaaca aatatgtatc
    10441 tataataact gtagttgcaa tgtttgttgg tggtattggt gtaatgaata taatgtatgt
    10501 atctgttatg gaaagacaaa gagaaattgg tataagacgt gctattggag caaaacctag
    10561 gtctatatta tttcaatttt tagttgaagc tgtttttata acagtatgtg gtggaatttt
    10621 aggaactata gttggatttg ctgctactaa ctatgtttct aaatatatag gttttgaagc
    10681 tatacctagc ttaaatagct tattttatgc aattgtagct actatattga ctggagttgt
    10741 atttggtctg attccagcct ttaaagcttc taaactagac cctataaagg caatttataa
    10801 gtagaaattg tataaagatt ttcgattttt agattagttt atataaaaca tacttaaata
    10861 tataacgcac tattatatat gatagattag gaacttaatc tttccaaata taatagtgcg
    10921 ttctttcata aaaaggattc tcttccttac tatatctaaa ttaccaagat gttatgttct
    10981 aattgttcaa actaatactt ccaattttga ttaatcaatc tcattctcct aatatacaat
    11041 cttttacttt caatcattgt acactccgcc ttaaaaaaat aatcttaaat taggcgttat
    11101 atacaatctc cccattacat attgtataat taacctttcc atataaagat ttacctttaa
    11161 atggtgaatt atcagcttta gaaacaaagc tttcaactgt ccattcttct tctggattga
    11221 atataacaat gtctgctaca gccccttctt ctataaatcc aatatttaaa ttgtacaatt
    11281 ttgcaggatt tatagacatt ttttctatta actgcatcat actaagatga tttttatgca
    11341 ccaaatttgt tattccaaga gctaaagaag tttcaagccc aataattcca cttggagctt
    11401 ttataaactc tctatctttt tcttctttac tatgaggtgc atggtctgtt gctattattt
    11461 caatagtacc atcatttaat ccctctataa tcttatctct gtcccattta cttcttagag
    11521 gtggattcat ttttgcattt gttccaaact ccaatacatc ttcctcagtc aatgtaaagt
    11581 gatgtggtga agcttctgca actacatttg cacctaagta ttttgcaaat ctaaccatat
    11641 caacagatac acctgaactt atatgttgta tatcaacttt agcaccagtc tttagtgcca
    11701 acatacaatc tctggctacc ataacatctt ctgctacact tgatgcacct tttaatccaa
    11761 gttctgaagc tacctttcct gcatttacac ctggatttcc aactaaagaa gggtcttctt
    11821 catgaaaact aagtggtaca tccaactctt ttgccttaat cattgcctcc ataattagat
    11881 tgctatccat taaaggtaat ccatcatcag taaaccctgg tacaccagct tttttaagag
    11941 cttccatatc aactaactct ttaccttcaa aacctttagt tatagctgct gcttgtaaaa
    12001 catttataca tgcatttttt gacttttctt taacataatt aaaagtatct tcattatcta
    12061 caataggatt agtatttgcc atacatataa cagttgtaaa tccacctcta gcagcagatt
    12121 tagctccact ttctatatct tctttatatg taaacccagg gtctctaaag tgtacatgta
    12181 catccactaa acctggagca actacattac cactagcatc tattattttt tcatattcat
    12241 cactttcatc aaacttgcct attttataaa ttttattttc ttttataatt aagtcaatat
    12301 tctcatctct ttgactttta gggtctatta ctctaccatt tttaattaaa atcatattat
    12361 ttctccttta tatttttaat ttatatagac tcatataata ttatacatta ttttacatac
    12421 ttaaatacaa aattacgttt gacatcaata gcctttttag gacaaagttc atggcaacag
    12481 taacatctta tacatttact taagtctata atagcaccct tagtccctat ctctatagtg
    12541 gatgcaggac aagcttcctt gcattttcca cattttatgc aatcttgaaa tcttaccaca
    12601 ggctttggtg ttataagctt tgaaataggt tcatttaaaa attttggtag ctttacggaa
    12661 aacaatctta aatctttact tacatttgga atcttgtaat tttttataat taaatctttt
    12721 atatcttcac ctaaaatttc aatatctgaa aaatcttctt gtatatatcc tctcttaata
    12781 gcacctctaa ttgtagcaac tttatttgga agtaaactta taatcttaca tgcaacaaca
    12841 tctatagcat atggattttg tgatgctaat aaaactccta tttctcgagt agtaccagct
    12901 gaaggtccat caccttccat acctactata ccatccataa ttgtcaatgt tggttttata
    12961 taatcacaaa tatctaaaag tacattttcg caaaacaact cttctgtagg aaatctataa
    13021 tgtatctctg cttttttcaa tccagctatg catccatata agttttttgt accaccagta
    13081 aaagttgcca ttatatgagt ctttaattta caaagattta ttatataatc tgaatctact
    13141 attggtttaa taatatccat atattttaga acatgtgctt tttcactatg tatcttaaca
    13201 tctgaaacat cataatttaa cttaatgccc agttcatttg ctacttcttc aattccagat
    13261 gccttataaa tgctcttaag agaactttga ttatatggac caccagggct atctgctatt
    13321 gtaatatcac aatttaactc taaaagtttt tcacatacaa cttttacaac cataggatgt
    13381 gtagttgtag cttcttcagg cttttttttc attaataaat ttggttttat aagtactttg
    13441 cttccttttt taacaaactt atctatacca ccaatatcct ctaaattttt ttcaatagat
    13501 aactttactt cttcataatt gtaagtttta caactcctaa cagataccga cttcatatta
    13561 tctccatcct tctatcacaa cagtttattt atataattta aaaaaggaga aaccaaagtt
    13621 tctcctttta atctatgttt taattgtttt aattattatt ttccaaaatg tctgtttaat
    13681 aatcctaaga atgctttacc atgtctagct tcgtctttac acatttcatg tactgtatca
    13741 tgtatagcat ctaatcctaa ttctttagct ctcttagcta attttaattt tccatcagtt
    13801 gcaccatact cagcatcaac tctaactctt aagttttctt ttgtatctgc aactacaact
    13861 tctccaagaa gttctgcaaa tttagcagca tgttcagcct cttcaaaagc tattctctta
    13921 tacgcttcag ctacttctgg ataaccttct ctatcagctt gtctactcat tgctaaatac
    13981 attcctactt ctgtacactc accagtaaag ttagctctta atccttcgat tatttctgca
    14041 tctacacctt gagctactcc tattctatgt tcatcagccc aaaccatttc gcctttcatt
    14101 tcttcaaatt tatcagctcc aactttacat actggacatt gtgctggtgc agcatctcct
    14161 tcatgtatat atccacatac tgtacaaaca aattttttca taattaattt cctcccaatt
    14221 ttatataatt aaatttttat ttttaagttt aataacttgc tacaagaata tatatacccc
    14281 agactttata gcttaaacac taaaaataaa atttatttcc taattaattt tatcactttg
    14341 aaacaccagt atcaaccaag ttttgatgtc aaatttctac ataatacata atttatttga
    14401 ttaattagga aatagttgtc atattaaata ctttctaatg aagactcctc tgaatcttta
    14461 agtttcactt ttttgctctt taaaaaagat aatttagttt ctgcagttat gattgcaaca
    14521 aatatcaaga tactaccaat aaccaatttt gctgtaataa gctcctttaa aattattata
    14581 gaaaatattg taccaaatac agcctctgtg gataatatta tagctgttct agttccatca
    14641 actctttttt gacatatagt ctgaaataaa aatccaattg tagtactaaa tacagctaga
    14701 tacatagttc ccatgtaacc agataattca gcttccatct ttaattgacc tgcaaatgtt
    14761 tgaactacaa cagacattaa aaacgctact gtaaattgaa tagctgtcaa tgccattggg
    14821 ttgttatctt ttgcaaactc acttgtaaag aatatatgaa atgcaaatcc gaatgaacat
    14881 ataagtgtta gaaaatctcc aaaattaata gagaaatcag cttctaaaga aagtatccca
    14941 attcctataa gagctaccaa actacttatt attcctattt tatcaagttt tcttctataa
    15001 agtataaatc ctatgaatgg aactattaca acattagctg ctgttataaa cgcattttta
    15061 gatggtgtag tgtacataag tccaatagtt tgtactgtga atgctagaaa taaaaatatt
    15121 cctaaaatcc ctcctgcttt taataatttt ttacccatat tggcacgaat ctgtttaaaa
    15181 aacagtaaat tcataattat agctgccaag aaaaatctta aagttatgat ttgtagaggt
    15241 gtaagcccac cgtctaatgc aaattgggtt gctacaaagc cacttcccca tataattgct
    15301 ataaatatta gtcctatttc tccaaagtat tttttcaata aagctccctc ctttttattt
    15361 ttttgcaaaa ttataaaccc cctaaacagc aagagagttt ttctataagc attactataa
    15421 attaatatag taaatttttt cagtatttta aatctaaatt tacatacatt ttgagctcaa
    15481 atttatatcc actatttaac agtaatatat taatcatttt ttgtaaacac ctttttaata
    15541 aatttttata ttttttatat ttttttacct atactaaatt tttttataga tatttttaat
    15601 atatatgata ctttaatatt atgcaaataa tgtcatataa gttacacttt agttatctta
    15661 gttacaactt tgtcggttta ctttttctac tttcttcgat ataattataa tatatttttg
    15721 atacaattcg acaaaagggg gaatgctgta attatggtaa aaaaaagaaa aaagaaaact
    15781 gttacactta ctatacttgt tgtaatagtt attggtattt tttctatggc atgggcggac
    15841 atgtctagaa aaaaagaaat gaaagaaact caagaaagac ttaaagctga aagattagaa
    15901 aaagaaagaa ttgaaaaaga aaaaaatgaa cctattattg gtgcaaaaaa agaagctgta
    15961 aaagaatatg gatatgacgc aaaaatagtt tgggacaaac ttaacaaata cgactattca
    16021 aacaatggag aaaagatagt ctttcttaca tttgatgatg gaccatctac tactaatact
    16081 ccacaagttc tagatatctt gaaaagacat ggtgttagag gaactttctt tattaagggt
    16141 gattctcttg aaagaaaagg agctaatgaa attttaaaaa gaacatttga cgaaggaaat
    16201 gcaatagccc atcactctta tactcatgac tataaaaaac tatatcctaa cagaagccta
    16261 aatctcgata cattcgtaaa tgaacttaac aaaactgatg aagctatgaa aaaagtctta
    16321 ggaaagaatt tttcctcaaa tgtagttaga tgcccaggcg ggtacatgtc atggaaaaat
    16381 atggaacctc taggaaacta cttaaaagag aaaaacatgg catcaattga ttggaacgct
    16441 ttaaatgctg atgcagaagg taaaaagaaa aatgcacaag aattatttga acatgcaaag
    16501 aaatcttctg aaggcaaaga aatggttgtt cttttaatgc atgatactta tggaaaacaa
    16561 gaaacagtaa atgcattgga ccaaattata acatatttta aagacaatgg ttatcaattt
    16621 aaaattttaa tataataaaa aagcaagttt attgaatgta cattttatac tcaatagact
    16681 tgcttttact ttactctatt ttactagctc gatttttaca aattttttct ttcctatttg
    16741 aacaactaat tcactctctt ttacaatagt gcttaaatcc tctacttttt caccatttac
    16801 ctttactcca ccttgagaag ctaatcttct aacttcactc ttactcttaa caatctcatt
    16861 agaaactagt acttcgatta agtcaaatcc atcttcctta acttgaattg tttgtatatc
    16921 ttcaggtatt tgaccttttt ggaatacaga tttaaatctt tcttcagctt ctttagcaga
    16981 ttcttctcca tggtataatg ttactatttc tcttgctaag ttcatcttta tatctcttgg
    17041 atttactgaa ccctctttca actgagattc tattttatta acttcatcag gatgaacatc
    17101 tgtcactaaa ttgtagtatt taattataag ttcatcagga atttccatag atttttggta
    17161 cattattccc gcttcttcat ctattccaat atagttacct aaactcttac tcattttttc
    17221 tttaccatct aatccttcta ttaaaggcat cattattact atttgagact ccataccaaa
    17281 ttctctttgt aatgatcttc ccattagtaa attgaatctt tggtcagttc caccaagttc
    17341 tatatcagct tctaatgcta tagagtcata tgcttgcatt aatgggtaga agaactcatg
    17401 tactgatatt ggcatttgtc cttcatatct ctttttaaaa tcttctcttt ctaacattct
    17461 cgcaacagta atagttgctg ctaattttat tacatcttca aaatttaact tagctaacca
    17521 ctcgctatta aatcttacaa tagttttttc tttatctaaa actttgaaaa tttgttcttc
    17581 ataagttttt gcatttgcaa gaacttgctc tgttgttaaa gcttttctag ctttagattt
    17641 tccagttggg tctccaattt ttcctgtaaa atctccaatt attattacta tttgatgacc
    17701 taaatcttgt aattgtttca tctttctaag aactactgta tgccctaaat gtatatctgg
    17761 agcacttggg tctagaccca attttactat cataggcttt ccttcttttt ctgatttaat
    17821 aagcttttcc cttaactctt tctcatctat taaatcatct acacctttca taattattcg
    17881 catttgctcg tctatcgatt tcatttttca ttcctcctaa aatatttata taatctataa
    17941 tttttatttt tataataaaa aaagctttca tcctcttaaa aggacgaaag cattgcttac
    18001 gtgttaccac cttagttcat caatatctca cgatattaat ctcttagagt acaattacat
    18061 atactcgtgt actataacgt gtactaatcc gatatctctt aatggttgtt atctttcaga
    18121 gatagtgctc tgagatgtat tcaaactaaa tcaatttatt ccatttcacc aaactggaac
    18181 tctctgttaa ataaaatcta gccctacttc ttctcttcat agcatttata tattcagatt
    18241 atttcatcaa ttacaagaaa tttttcttaa cttactcata atatattatt atttttatat
    18301 tttgtcaacc ttttttatta ttatatacat gtttttttac attatactac ttttttatat
    18361 tttttaccaa tacccaaaat ttatttttca atcaaaagat ttatttgttt attgactata
    18421 ttactctgac tcaatcttat atttattttc ttatttaaaa atgcgtttat aattattaaa
    18481 ctaataactg ttacatcaaa tataaaaaac agcaataaaa ttattatcaa aaataaaatt
    18541 ctagcatata ataattttct aaacttagct attataaagt tttgtagtga catacatgag
    18601 tctctaatga taaaatagac acttatatag gttattgata taaagattgt ttttaaataa
    18661 acagaatcaa ctttaacatt caacagtttt agaacttcaa aaatggctag ataagaaaca
    18721 catatgtaaa aagctttttt tgagcaacat aaaaattggt ttaaatttct aaagttctta
    18781 aatattaaaa atttttttaa agaattagaa tctaaaatat gcaatttatt ccctaaaatt
    18841 aaacttaata aatatatgca aaacaccata ccaaaaggaa acaatgctat cattaaataa
    18901 taaataaaat ttgtataccc attaaaataa atccatttgc tatatccttg aaattctttc
    18961 ataaaactat aacctattaa tgttgaagca tatatatcaa tgaaaaccat tacaataacc
    19021 ccaaatactg aaccatatac taaatcatcc ataaccattg taggcttatt tattagatag
    19081 ccacacatta taccaagtaa tacactctga cttgcccaaa tagcagttcc tagatttcca
    19141 agtaccagag aaattattaa aagcgaagta attccagata aaatggtata ctttaaatca
    19201 catttcaagc agattataca aaaaaatact ggcactataa aatctaagta aatagcatat
    19261 ccaaatccag tactcacagc tataattgtt actactatga ataaagatga caataaagct
    19321 gcttcagtta attttttagt agtattcaaa acactgcctc cataaatcat aaattttcta
    19381 actaaagttt tgtttttaat atagtatatc actttttaaa aatattttta ataaatataa
    19441 attttaataa aggtatttat gttaaaaatg gtataatgta tcttaatatt cagtttttta
    19501 agatgctagg aggtaattac tatgtttaaa attaaacact ttaatgattt gagtttagat
    19561 gaattttatg aaatagcaaa atcaagatat gaggtatttg cttgtgaaca gaagatattt
    19621 tcattaaacg attatgatga tatagataaa agctcttatc acatattttt aaaagaaaat
    19681 ggattgattt gtgcttacgc tagaataatt cctaaagaat acagctctta taatgatgtt
    19741 tccattggta gagttttagt tctttctagt catagaagaa aaggattagc aaaacaaatg
    19801 atggattgtg ctatagattt tataaaagtt aacttacacg aaaataatat aaccttatca
    19861 gcacaaactt atataaagaa cctttactta tcttgtggtt ttaaagaaat ctcagaagta
    19921 tacgacgaag caggtattga acatattaaa atgagattat agtgttgaca agctaatatt
    19981 gttaatatat aatttaagta taaattaaat attgaattga tatttaggta tttgaaaata
    20041 ccttacatag agtcagaagg cttttgcttt ctgacttttt tattatcaaa attcaataat
    20101 tatatggatt caaagtgtta atttttattc tatattctac tttccccaaa agtaagaatt
    20161 atacccaact ttgtatgata ggcatcttgc ctatcttttt tttgtaaaag taacaaggta
    20221 ataatataat gcatagttta aaataagaag attagcccag gctaaatttc aagcatggag
    20281 gtgctattat gaaaaacctt aacgatattc aagtgaaaaa aactgtcaaa gttgaagata
    20341 tattatctag tggtaattta agagaaagaa tgttagcgct tggtcttact agaggagctg
    20401 ttatagatgt agttagaaaa ggtccaaaaa ataatttaac tgtctataaa atcagaggta
    20461 gcaagatagc tctacgacaa gaagaaagta gcttaatatt agtttctgac atttaaaaat
    20521 ttctagcagg aggatattaa tatggggttg acacataact caactaaaat gagttcttta
    20581 aaagacatgt ttgatataga caataaggaa gaccagtttg taattgcatt ggctggcaat
    20641 ccaaatacag gtaaaagtac tgtatttaat catcttacag gacttagaca gcatactgga
    20701 aactggccag gaaaaactgt agctacagct cgtggtaatt ttaaatataa aaataccgaa
    20761 tatgcattaa tagatttacc aggaacatat tctttatttg ccttatcaca agaagaaatt
    20821 gtagctcgtg attttatatg ttttggtaat ccagatgcag taatagtagt atgtgatgct
    20881 acatgtcttg aaagaaattt aaatttagta tttcaagtga tggaacttac agataaagtt
    20941 attttgtgta ttaatttaat agatgaagct agaaaaaaag gcattactat tgataaaaaa
    21001 cttttagagg attctcttgg tattccagtt attttaactg ctgcaagaaa tggctctggt
    21061 atggatgaac tattggatac tttaaatgat gtatcttttg acaaatataa attaaataat
    21121 aaacctgtta gatacaatga aaatatagag aatgtggtaa aatcaattca accagaattg
    21181 gataatatta taccaggtat aaattctaga tggttgggac ttagacttat tgatggcgat
    21241 gaaagtatat ttgaatctat gagtaattat atagacaaag attctataga tgctattaat
    21301 gaagtaaaga aaaagattcc agacaatata aataaacaga aaataagaga tgagtttact
    21361 aaaatcaact atgattatgc taaaaaactg agtgatgagt gttgttctaa tgttgctaaa
    21421 aaatcaactg atagagaaga aaaagtagat aaaatattaa catctaaaat atttgggctt
    21481 ccaataatgc tcttattatt aggtactata ttgtggatta ctatagaagg agctaactat
    21541 ccatccactt tattatctaa tttattactc ggatttgagc caagtatttc gggtatatta
    21601 aatagtatta attgtccatc atggttaaat gatatgttgg ttttaggatt atacagaaca
    21661 ttagcatggg taatctctgt aatgctacct cctatggcaa tattttttcc attatttacc
    21721 ctacttgagg attttggata tttgccaaga gttgcattta acttagacca cctatttaaa
    21781 aaagcttgtg cacatggaaa acagtgtttg actatgtgta tgggatttgg atgtaatgca
    21841 gctggagtta ttggttgtag gataattgac tctccaaggg aaagacttat agctatactt
    21901 acaaacaact ttgttccttg taatggtcgt tttcctactt tgatagctat atctacaata
    21961 ttttttagct cagttataac caactctttc gtatcaagcg tagcaactgc actttgcata
    22021 actttactga taattctagg tgttataata acattgttgg tttcttatac tctttctaaa
    22081 acgctattaa aaggagttcc gtctacattt acattagaac ttcctcctta tagagttcct
    22141 caaataggaa gaactttata tacctctata atagatagaa caatatttgt tttaggaagg
    22201 gctgtaatgg tagccatacc tgctggtgtc ataacttgga tatttgcaaa tatatatata
    22261 ggagatttaa gtatattatc acatgttgca aactttttag accctttagc taaattaatt
    22321 ggtcttgatg gttttatatt acttgctttt attcttggat tcccagcaaa tgagatagtt
    22381 gtacctatac ttttaatggc ttacctagca actggttcta tgatagaatt agatagcttt
    22441 agtgctttag gtcaagttct tagagaacat ggatggactt atttgacagc tttaaatgtt
    22501 atgctgttca gtttactgca ttggccttgt gcaacaacac ttttaacaat aaaaaaagaa
    22561 actggtagtt taaaatggac tgccttagga ttcttaatgc ctactatact tgcttttgtt
    22621 gtttgttttt taacaactac agtttacaat ttatttatat aattagtatt aactgacagt
    22681 agacatatat tagagaaacc atttaaaaat ttatatactt tattataaag catagagata
    22741 tattcaatga taagtaaaat tacttaacat ttgaatatat ctctatttta attcttatct
    22801 ggatacttgg attgaaattt cagtcatata attatcttca ttactttcac aaaaaaagtc
    22861 aagtatactc tcctcatacg aatttccaac aattttatat tcattttcat atatatattt
    22921 tttcaatttt tcgtaagatt tatatgaatc ctcatatttt ccaatatgtt ttatgcaagc
    22981 atacattcct tctggcttta catatagctt tttatccttt atttttttat ctatcttcaa
    23041 ataaaaactt gatatactta aatagtcccc attttttata ttttcattac ttactattac
    23101 tcccaactca tatccttgat acaaattttt cgatttacaa taattggtaa aatctattaa
    23161 ttccatcata aaatcactat tactcatatt actcaaatcg cttgatagaa ctaaatattc
    23221 ttcaaacttt ttggatatat aaatttcttc actattcatt ttagaaattc cttcaactgt
    23281 tatatttatt ctattttgca tcttagaact tatctttctt agttttttta ttttttctct
    23341 tatctttttt tcttcttctt taagcatttt cacacaaagc tggggagttc tattttgtat
    23401 atactctttt atttcctcta aactcattcc tatttctctt aatgattgta atatatcaag
    23461 tgtatcatat tgataaactg agtaatatct ataattattt ttatctttat attcaggaga
    23521 aaaaatacct attttgtcat agaaaattaa tgtctgctta gatataccac acaatttagc
    23581 aaattcacct gttgtaaaat actgtttttc caagggttat acactccttt tctttttgtt
    23641 gtctatatag ttactatatt ctttattatt tactaaatca agtagttatt attttagaaa
    23701 atcaatatgg ttttgatttt aaagcttaaa tatcagttag atgtcagtta taaatatttt
    23761 acccattccc atcataaaaa agatatcccc tcttaacttt tggatggaat attatattta
    23821 tacttgttag agtgaaaatt gtaataggtg ctacaataag atatttaatt aaacataaag
    23881 ttaattattg taataaaaaa tctttagata aattagagtt aaagtatgga aatatagata
    23941 gagaaaaagc tgttaaacta gaaatatttt atcaatattt aatagaactt gaatatatag
    24001 aaatgagttt attaataaga agattagata ctgcaataac atcattagca ttagtatcta
    24061 ttataacaat agca
//
LOCUS       DAMJWU010000049        23436 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMJWU010000049 DAMJWU010000000
VERSION     DAMJWU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.049"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(58..822)
                     /locus_tag="PC460_002167"
     CDS             complement(58..822)
                     /locus_tag="PC460_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2601051.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            1096..1770
                     /gene="fsa"
                     /locus_tag="PC460_002168"
     CDS             1096..1770
                     /gene="fsa"
                     /locus_tag="PC460_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2601052.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1892..3286)
                     /locus_tag="PC460_002169"
     CDS             complement(1892..3286)
                     /locus_tag="PC460_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF2601053.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFTFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3364..4710)
                     /locus_tag="PC460_002170"
     CDS             complement(3364..4710)
                     /locus_tag="PC460_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2601054.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      4960..5062
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5156..6532
                     /locus_tag="PC460_002171"
     CDS             5156..6532
                     /locus_tag="PC460_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2601055.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(6646..7791)
                     /locus_tag="PC460_002172"
     CDS             complement(6646..7791)
                     /locus_tag="PC460_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2601056.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            8044..8751
                     /locus_tag="PC460_002173"
     CDS             8044..8751
                     /locus_tag="PC460_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601057.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            8798..10393
                     /locus_tag="PC460_002174"
     CDS             8798..10393
                     /locus_tag="PC460_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601058.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMLIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            10496..11578
                     /locus_tag="PC460_002175"
     CDS             10496..11578
                     /locus_tag="PC460_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2601059.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            11727..11888
                     /locus_tag="PC460_002176"
     CDS             11727..11888
                     /locus_tag="PC460_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF2601060.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            12229..13497
                     /locus_tag="PC460_002177"
     CDS             12229..13497
                     /locus_tag="PC460_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2601061.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            13692..14480
                     /locus_tag="PC460_002178"
     CDS             13692..14480
                     /locus_tag="PC460_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2601062.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            14480..14929
                     /locus_tag="PC460_002179"
     CDS             14480..14929
                     /locus_tag="PC460_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2601063.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            14933..17197
                     /locus_tag="PC460_002180"
     CDS             14933..17197
                     /locus_tag="PC460_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2601064.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVK
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGRMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            17337..17648
                     /locus_tag="PC460_002181"
     CDS             17337..17648
                     /locus_tag="PC460_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601065.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            17934..18434
                     /locus_tag="PC460_002182"
     CDS             17934..18434
                     /locus_tag="PC460_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF2601066.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            18491..19477
                     /locus_tag="PC460_002183"
     CDS             18491..19477
                     /locus_tag="PC460_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2601067.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            19527..20249
                     /locus_tag="PC460_002184"
     CDS             19527..20249
                     /locus_tag="PC460_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF2601068.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            20278..22212
                     /locus_tag="PC460_002185"
     CDS             20278..22212
                     /locus_tag="PC460_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2601069.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKQEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            22205..>23436
                     /locus_tag="PC460_002186"
     CDS             22205..>23436
                     /locus_tag="PC460_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF2601070.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLILLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTK"
BASE COUNT         9004 a         2511 c         3788 g         8133 t
ORIGIN      
        1 tatttatcaa ataaaaattt atatttataa gaaatcctat aaaacatata attaaactta
       61 taaaacattt acattaaatt ctgataaaat atctactata tcaggagtag gcgttttttc
      121 tgtaattata gaactaatat cttctaaatg agcaaacttg aaatttccat caaatttaaa
      181 ctttttatct tccataacta taaatacttt tttacttgaa tttattatag ccttcttcgt
      241 aagaccatct tcaatttcaa aagttgtaag tgaagagttg aatacatcta ctccacaact
      301 tcccataaaa gtcttatcaa acttatattt cgatataaac tctatagttg gagtaccgac
      361 aaaaccgtct aaactgagat ttaataaacc tccagtggaa attatattta aattatttgg
      421 ttttgaagta actacattta gtatatccag catatttgta acaagtgtta cttttttatt
      481 actttcagct aacaacttcg caagcaagat gttaattgta gagatatcta aaaatatagt
      541 ttctctatct tcaattaaat taaatgcttt ttctgctatt attttctttg ttggaatatt
      601 aatttctttt ctgtcttttg tatcctgatt ctctagtgtt tctctagcta gtacagctcc
      661 tccataaatt cttcttattg aagaaatctt ttctatattt tttaaatctt ttcttataca
      721 atcttctgta acattaaatt tagaactgag ctctttgaca gtaactctac cttctttttt
      781 taggatattt aatatagctt ggattctttc ttctgcaaac attttctacc tcttttcgca
      841 atatagtatt atagtaaata ttataagata aaatacaata tatcaactat tttaacatga
      901 aatacaaaat taatttaata taaaaagtat gttttttcta actatatatt tagaatttag
      961 taaataaaaa aattataaaa ctaagaagga aaagtaaaac aataataaaa aataaaaaac
     1021 aataagcaaa atgttgaaaa taataccaaa tcaatatata ataagaataa agaaagatta
     1081 aagggggata ttttaatgca actaatctta gatacaggta atgtggagga gattaaggag
     1141 ttatgtactt gtttaccaat agatggtgta actacaaatc caagtattgt ttcaaaagaa
     1201 aaaaagaatt ttaagcaact tataaatgaa attggtgaga ttataggtga ggatatgcct
     1261 atacatgctc aagtgttaag tacaaagtat gaagaaatat tagaagaagc attatatata
     1321 agtagtttga gaaaaaatat atatgtaaaa atacctgtaa cacaagatgg tttaagagct
     1381 ataaaagacc ttcatagaaa aggtgtaaaa ataactgcta ctgcaatatt cactgctcat
     1441 caaggttttt tagctgcaaa ggctggagca aattatattg caccttatgt aaataggctt
     1501 gataatatat ctggtgatgg agtagctatg gtttcagaac ttataaaaat aatagatact
     1561 tataaaatgg atacaaaagt acttgcagcc agttttaaaa atgcacaaca agttatagaa
     1621 ttaatgcaac atggggtgca tagtgttaca gtaccatatg atatttgtaa atctatgatg
     1681 aatcatccat taactgattg gagtgtagat aagtttatag aggattggga gaatactttt
     1741 ggaaaaggaa gtaaaacaaa taatatttag tattatggta aaagagtagt tttgtccaat
     1801 tttacaagaa cttatataaa aagatgaaaa tatgaatttt ctttttaata atttcgttat
     1861 tatcttaagt taattcatat tttcatcttt tttagatttc tagtcctata aatttccacc
     1921 aagggaatgt tatacatatt attataaagt aggttaaagg aatcaaagtt aaaccgtatt
     1981 tgaaaataac cttgctatta tacagatttt caccatgacc aaccattatt gtagcatgat
     2041 gaaatggtaa tatgtactgt atgcttacta taatgtagac aaatagacat aaagtaacac
     2101 tattttcctt tagtatatgt aaacttccaa gtaatggtat tactacagat gatgtagtga
     2161 cagcacttcc aagacatata ttcaagatta ttgtgagagt tatcaaaaac aatattgaaa
     2221 taatttctga agagtttggt ataaacttta tcaaataatt tcccattata tctacaactc
     2281 cagagtactt taacactcct ccaattgaaa atgctgctat aaaaaaaata aggaccttcc
     2341 aatttatttc ttttaaagta ctaaatccaa ctatttttct tagtaataaa agaagtacac
     2401 aaattgacat tataattaat gtgtttatgt tatgtatgaa ctgagttaaa aacataaaaa
     2461 acattattcc taggcataaa aatacgtgtt tttgttcttt gtttagcaaa ttaaattctt
     2521 tttttaaatt agaatatgaa aaatgaagtt tattaccctc aagattttca ttagaaataa
     2581 attttattga atgcaattct tttttaaaga caaaggtaaa taaaaaataa gttataaaac
     2641 aagtaattat agttggaaca gacatataaa aagcccattg accccaattt atattacaat
     2701 ttccaagttc tagtacaaca taatttagaa gagtgtctcc atttacaaaa aacattgatg
     2761 taaaggtaga tgctgtaaat atactaaata aaagtataga ttttgaatct tcagttatct
     2821 gttgttcttt taaaaactcc ttgtaaaaag cagatacaag aataaccctt ggaaatggtt
     2881 gtgggataaa aaatattaaa agtataccta aaatatacga taaaagaatc atttttttag
     2941 gggtatttac agtatttaaa attaacttct cagaaataat ttttgcgaca cctgtttttg
     3001 taactgcatt agtaattatg tatgcaagaa ttattatgta aaaattttct gtaaataaaa
     3061 actgtaaaat aacctttatt ggtgcaatgt taaatataaa cgctagaatt acaaaaaaca
     3121 atgcaacaaa agttttttca actgagtcta cagcccatat caagatagtt aaaaaagata
     3181 agcatataga aaattttatg ttgaaactgt aattaagaga gaagtagaga attatggtag
     3241 ctataacagt catataaaat tgttttggct taagaataat acccattttt atattcacct
     3301 ccataattta ttatatcata atggaggtga atacaatgaa tgtgaataca ttttataaaa
     3361 agattattca ttgattctat gatttacaat agctttagtt ttccaatgtc ctttcttata
     3421 gtagataact gtaacaataa gacctaaagt ccaaccaaaa ggaataccca tccaaattcc
     3481 gtctgttcca aaaaatttag aaagcatata agaagctgga agtcttccaa gccataaaga
     3541 tactattgag cttataagtg caaacatgga atcaccagca cctttcattg cacttgaaag
     3601 tacaaaagaa gttccaataa ataagaagaa tggtcctatt ataaataagt aagaagagcc
     3661 aattttaatg acatctttat cagtattgaa tagagcaatt agattttccc taaatagcaa
     3721 aattaaagca ataactgtaa ctgctaaaac taaggtcatt tttatagatt ctcgaatgcc
     3781 ttttttaact ctttcatttt ctccagcacc tatgttttga gcaacaaaag tagaaacagc
     3841 agaaccaaga ttcataatag gtaagcttat aaaattttct atcctaagac cagcaccaaa
     3901 agctgccata gcagatgtac cataactatt aactaaaaat tgaagagcca tatttccaat
     3961 tgaaaacaac atttgttgaa ctccacttgg aagtcctaat ctaagtccat ctttaaatat
     4021 tttgttatca tatacaattc ccttgatttt aaatttaagc acttcatgtt ttttatttaa
     4081 ataaattata cttattataa atgatactgc ctgagccatt atagtggcaa gtgcaacgcc
     4141 ctcaacaccc attcttagca ctacaataaa agttaagtct aaaactatat ttagaattgt
     4201 ggctattatc agaaaataaa gtggagttac tgaatctcca agtcctctaa gtattgcact
     4261 tactgaatta tatccagcag aaaataatat tccactaaat attatagtaa gatacaattt
     4321 tgattgagca aaaacggatt ctggtgtatt cataagctta agcattgggc caccaaatac
     4381 tattccaaga atacttatta atatagaacc aataaataaa agtatatatg tagtatcaat
     4441 agtagctttt acctttttta aatctcctga gccataaaat tgtgacacta aaatagaaaa
     4501 tccaaggcta acacccataa gcgctgccac caataaaaac attatagggt tagctccaga
     4561 gactgccgcc attgcttctt ttccaataaa tctaccaact attatactgt cagctgtatt
     4621 gtatagttgt tgaaataaac taccaataag cataggcatg gcaaaaaaga atatggactt
     4681 tccctcatga cctgtagtta aatctttcac cttattcctc caaataaaaa aattattaat
     4741 ttattatagt ataatcttaa cataaacttt atatccaaac tataaataaa ttgtatcaaa
     4801 atatcatata tttttttaaa atgtaaagaa tataattata atttgaattt aataaatcaa
     4861 tatttaatat aatcgaatat taatacaaaa tcaatacaat atatacgtat atattgacaa
     4921 aattaattat ataaattaaa ataatattcg taaagaaaaa ttcatgtttt gtactgtata
     4981 acatataatt ttgacaatat gggtcataag tttctaccgg aataccgtaa atattctgac
     5041 tatgtatact attatttagt taattaaata tttaattaac tgaattatga aggatacggc
     5101 tcagcttatc aactactgtg tcgttttttt gtaaaattta ataggaggtg ttgttatgca
     5161 aacaacaaac agtagtacta tgcagaaact ttttccaatt ttgactaata aaaatgtcga
     5221 catgaagaag gaaataattg caggagtaac tactttttta actatggctt atataatagc
     5281 tgtaaatcca aatattttat ctgagacagg catgcctgca ggtgcacttg ttacaggtac
     5341 ttgtttagca gcagcatttg gatgtatttt aatgggtgta gttgctaacc ttccttttgc
     5401 tttagcatca ggtatgggtc ttaatgcatt ttttgcatat acagtagttt tacaaatggg
     5461 tgttccttgg gaagtagctc ttacagcagt atttgtagaa ggtattattt ttatagtatt
     5521 atcagtaagt ggagttcgtg aggctgttgt aaatgcgata ccaaaaaata tgaaattagc
     5581 tgttacaggt ggtataggaa tatttattgc acttataggt ctagttaaca gtggaattgt
     5641 tattggagac caagccactc ttatcaaaat gggtagattt acaccagcag taataataac
     5701 ttgtgtagga ttaataatta ttgctgtatt agataaaaaa agagttaaag gttctatact
     5761 ttttggaata gtagtgagtt cattacttgc ttgggcattt gcttttataa atccagagta
     5821 tgcacaaaaa ttaggtatat atttacctgg tggattattt aaattcgaaa gtttagcacc
     5881 aatagcaggt aaaatagatt taggatatgt actacatcca acaaatatag gtggtttttt
     5941 agtagtagtt tgtacatttt tatttgttga tttttttgat acagtaggta cattagttgg
     6001 tgtttgttct aaagcaaaca tgttagatga aaaagggaat gttcctaatg taggtagagc
     6061 cttacttaca gatgcggttg caactacagt tggtgcagga cttggagtat ctacagttac
     6121 aacttatgtt gagagttcaa caggagttat cgcaggtgga agaactggat ggacagcaat
     6181 aacagttgga attttattct tagctgcaat gtttttctct cctgtattta tagcaattcc
     6241 atcatgtgct acagcaccag ctttaatata tgttggatat ttaatgcttg gtacagttaa
     6301 ggatatagag tttgataata taactgaagg tgttccagct tttgttacta ttgcttgtat
     6361 ggcattaaca tatagtatag gtgatggttt aactttaggt atattaactt atgttttcgt
     6421 aaatatattt tataatatat ttggagctaa aaaggcagaa gacaaaaaac atgtttctgt
     6481 agtaatgatt gttctggcaa tattatttat aataaaactt tggctactat aataggtatt
     6541 atttaaaatt ttaatttaat ataaaataaa gggctgtaat ttattatcaa ttgtgatgat
     6601 aaactacagc tcttttgtat ttgttgtatt tttgtattta ttgtattatt taataatgtc
     6661 tattagttct ccattttgta aagttttagg tgtaattact atattgaact ctttttgcaa
     6721 atcttttata atatctaatt ctttttttgt tattattgag attgtgttac cacttcgatt
     6781 tcctctagca gttcgaccac atctatgcag gtattcattt ttactttttg gaaaatccaa
     6841 gttaaaaaca tgacttacat ccacaatatc tagtccacga gcagataaat cagttgtaat
     6901 taaaatcttt gctttaccaa gtttaaattt atttattgca ttctttctat cttctttttc
     6961 catatttcca aaaattccta ttgctttgta attatgataa tttagttttg atactaggac
     7021 ttctatattt ttttcattat ttacaaatac aatagctttt tttggatttg tagctgctaa
     7081 tgcctttcta agaaaagtaa atttatccct tatctcacct aataaatatg agtgattgat
     7141 gttaggattt atttgtgatt tttcctttgt ttttattatt tcaaactcct tcataagttt
     7201 atcacaaatt tttatagtgc tatcagtaag acttgcagag catcctataa tttgtctatc
     7261 tcttaacgtt gttcttatta tgtcttctat acaagttata ttttttccat tcaatagatt
     7321 gtccacttca tctaagacaa tagtttttat attatgtgat tttagtttct tttgttttat
     7381 caagtctaaa actcttccac aagagccaat tactatgtgt ggttttactg cttttatatt
     7441 ttttatttgt ttttgtatat tgacctcccc aattaaagct aaagaagtaa cggagagttc
     7501 tgcattttta gccaataatt caacctggtt tgtaatctgc attactaatt catgcgtagg
     7561 tgcaagtata agagcttgtg tttctctttt tgatgtatca attttttcaa atataggtaa
     7621 aaggtacgct agtgtttttc ctgttccagt ctgtgaatta atcaataaat ccttattttg
     7681 aattatattc cctataacaa gtgactgaac ttctgttgga gaagttatgt cttgtttttt
     7741 gagtccatct attagagtgg aacttatttt taattgttca aatgtattca tattttactc
     7801 cttatatttt aataattata gtcaactatt ttttattata cattgtttat tcaaatcgaa
     7861 ctatatattc ttttataatt atgtgtaatt tgaaagtaaa tatcaattat atttatacct
     7921 ttatatttct gatacctttg cattttatag atttctaaaa taaacaaata aaagttgtaa
     7981 ttgtttgata tttgttatat attgtatata acaaatatca aaactataat atggagggaa
     8041 attatgttaa aagtaatcaa tgtatctaaa tcatataaaa ataaaaaagt agtaagtagt
     8101 gtctcatttg atgtaaatga tggagagctt tttgggttta taggacataa tggagcaggt
     8161 aaaactacta ctattaaagc aattgttgga atacacgatt ttgaagaggg agaagtacta
     8221 ataaattcta aatctataaa aaaacagcct gttgaatgta aaaaggaaat ggcatatata
     8281 cctgataatc cagatttata cgagtcatta actggtatac aataccttaa ttttatagct
     8341 gacatatttg aagttgccaa agataaaaga gaagagttaa taaaatatta ttcaaataag
     8401 tttgaactca ataaagcatt aggagattta atatcttcat attcgcatgg tatgaaacaa
     8461 aaattagcta taatttcggc tttgatacac agtccaaaaa tcttaattct tgatgaacct
     8521 tttgtaggtc ttgacccaaa agcttccttt atattgaagg aaattatgag ggagttttgt
     8581 gacaaaggtg gttgtatatt tttctcaaca cacgttttag aagttgctga aaaaatttgt
     8641 gataagattg ccataataaa agatggaaat ataattgctt atggaactac agaagaagtt
     8701 aagggaagta attcacttga aagtatcttt atggaattaa tagagaaata aaatgttttt
     8761 tatacaatat agtgtctaaa gtgaggaatg ataaaatatg agcaatctaa gtatattaat
     8821 aaaaactaat ataataaatg aatttaaatt aaatgcgtta aaaaaagctg atggtagaga
     8881 aaaacaaaaa ataatgggaa tgatgctttc agttgttata atagcgggtc tattaatttt
     8941 ttatgtaaca agcttaagtc tctctttagt tgatatctta aagcaaataa atcaactgga
     9001 aatgctactg atatttggtt ttggattatc aacattaaca actattgtta catcattata
     9061 taaaacgtca tcatatctat ttcaagcaaa agatttagac cttttgacta gtttacctat
     9121 aaaagaatct actgtactag caagtaagat attaatgttg gttggagcaa actatttatt
     9181 ttcttcaata tgtatgctta tacctgcagt ggtttacttt ttaaattctg atgtaagtat
     9241 aacttatttc ccttatttga tattgctatt tttagcttta ccattattac cgatagttgc
     9301 ttcatcaata atttttctat ttatagggca gatatcatca agaataaaat acaaaaattt
     9361 agtattaatc ataggtagca ttatattgat gttgctttat accctactta tgtcaagact
     9421 taattctatg tcagttgaaa tgctaaagaa tagtgtgtct atttcagaga cgattagaaa
     9481 agtatatcct cctatatatt attttgttga tgctcttaaa acaggaagta tagtttcatt
     9541 tttagcattt atagccatct caataatacc attttttatg tttgtaactc tgtttgcaaa
     9601 aaaatttaaa gatataaatg ctaagatgag tgaaggatat aagtcaaaaa attatgaatt
     9661 taagggtcaa aaaacttcaa gaccttcaaa agctttatta aaaaaagaat ttaagagata
     9721 tacttcttcg tttatatact ttttaaattc atctgcaggt ttgttatttc taatagtagg
     9781 tacaatttta atattattat ttggagcaga taaagtgggt gagttactga aattgaatat
     9841 agattttaat cttataaaaa tacaattatt gggagtattc ttctttataa tagtaatgaa
     9901 ttgtacaaca tactgttcaa tttctcttga aggaaaaaac ttatggatac tgaaatcttc
     9961 tccaatagat gaaagagaaa tattctggtc aaaaatattg atgaattttt tattgaatgc
    10021 accactatct attttatgtc taatattagt atctataaga ttagactttg aaattcagtt
    10081 tattattgta tcaatctgtt tgattgtaag tttttcaatg tttgtagcat tgatgggatt
    10141 attatcaaat ttattatacc caaaccttga ttggaaaaat gaggtggctg tagtaaaaag
    10201 aagtgcaagt atgatagtta caatgttagt atcattagct tatgtaatta tacttggagg
    10261 tatatatgta ttacttggaa tttcgttttt aaatatatat attattttgg caagtatact
    10321 tactttgata ttagatttta tgatatggag aattatttct actaaaggag taagaatatt
    10381 taaaaattta taggtgtatt tatgtcaaat aaagacttat cttttttagt cttgcaaata
    10441 ataggttaag tagtatacta tgtatagtga tatgaaaaaa tttaggagaa aataaatgag
    10501 aaaattatcc aaaagagaaa aagattttat tcaatatgga tttttattat ttttgatatg
    10561 tattactact tatttagtat taactacttt agatataaaa ctaataccta aaattataag
    10621 gctagtaaat aagacttata tatttcttgg catattgaca attatagtat atatgataat
    10681 tgaatctatt gtaactcatt tgataataaa tgcagttcaa aaaacaaaag taaaatttat
    10741 tggtattaaa atggctacaa tgggatttta ctataactta gttactccat ttgcatctgg
    10801 gagtcaacca atgcaaatat atgcacttac aaaatatgat gtaagcctta gtaaatcagt
    10861 ggctatagtt actaataaaa ctgtagtatt tcaaagtaca gttactgtat tttgtggaat
    10921 tttaattttg ctaaataagt cattactgat ggagcaagta cattctgtga aagtcttgat
    10981 aacttttgga atgattatga atgtaagtat gttgttaggt ggactactaa tagtatttag
    11041 tccaaaacaa gtaaaaatga tagcagaagt tttgattaat tttttatcta aatttaaaac
    11101 atttaaattt ttagattcaa aacgtgatag aataaatcat tatattgatg attataatta
    11161 ttctataaaa atatttatta aaaatgtaag aatcttagtt ttaagtatat tacttactgt
    11221 gttacaatta atcgcatatt tcagtatagt ttattgtgtg tataaagcct caaatttaag
    11281 aggagtttca tatgtacata taatgacatt acaggtcttt ttatatatgg ctatatcacc
    11341 aattccaaca ccaggaaatg ttggagctaa tgaggttaca ttttttacta tgttctccaa
    11401 tataattcca aaagaattat tggggtactc tgtactctta tatagtgggt ttgtatacta
    11461 ttttatatta atagtttcag gtttatttac atttggaacg cattatacat taaattcttg
    11521 gaaaaaggaa aataaagtaa attctaattt agataaggtt attgaaaaat taaattaata
    11581 aaataattag ggaaattcta tatataaata tggtgttgaa taagctatat tatattgaga
    11641 tatttccctt ttttgattaa aattatagaa atttgaaata ataagtaata aacagaaaaa
    11701 gtgatattgt tttgaaagga gaaactatga aacaagataa acaaaatgaa aaacaaagtg
    11761 agatttctaa gataaaacaa agcaatgata ataaacttga aatagctcaa gaattacatg
    11821 taagtaaaaa tatagggaat atgcttggat atagaataga taagaaaaaa atgaatagac
    11881 caagttaatg aaaatgttga aaatggtaca ttttaaaaaa tatatataat gaaatgtttt
    11941 attgttaaat ttaaacttat tttaatgggg gaaaaagtcg aaaaatatct aaaaagatat
    12001 ggatataaaa aatattaata tatatatact ataaacttga caaaatcaac cttttaataa
    12061 ctagcaaaaa atagcaaaat tatataagcg ttatttaatt gtcaaaaatg aaagctaaat
    12121 tgcatgtcct aaaaaaaatg attgataaca tttatttgtg tgctataatt atatagtaaa
    12181 acaataagta tattgcataa tgtatgcaaa tcgggaggag agacggacat ggaagcatct
    12241 atacacacaa ttgccagcaa taatttattg ataatttttg ctattgtaag tataactgga
    12301 ataatttgta gtaagcttag tgaaataata aaggttccag atgtagtgtt atatttactt
    12361 gtaggtattt taataggacc atcattttta aaatttatag atattcgtgg atttcaaata
    12421 gaaaatcaac taatacttac atttggttct gcatttattt tatatttagg tggaaaagaa
    12481 ataagtttaa aagtattgag aaatgtgaag atatctgtat tcttattagc tactttagga
    12541 gttgtgatat ctgcttttat aatgcaacaa gtaataggat ttacttttgg gataagtgca
    12601 atgactgctt tactagcagg aactataata gcatcaacag acccagccac tcttgtacct
    12661 atatttaatc aggtaaagat aaaggatagg gtaaaacaaa ctgtaataag tgaatcagct
    12721 tttaatgatg caacaggagc tatactaaca tcagcagtgt tggctgtaat tttgtcaaat
    12781 aaattttcat taggtgaaaa tatttatgaa ctggggacaa tggttattgt cggagtttta
    12841 gttggtttaa ttacaggaat actgcttttg aaattagtta atgataaacc ttatggaata
    12901 tttaaagatt ttgcaccaat aatatctgtt atttcagttg tagttgcata tgaattatct
    12961 acaaaattag gtggaagtgg atatatgtct tgctttattg taggtataat aactggtaac
    13021 aagaaaaact ttaaaatttg gttaagtcaa aaatcttatg atgcagattt ttatgttgct
    13081 gaaactttag gtactatttg tcgtatggca atatttataa tattgggtag ccaagttaat
    13141 ttggctcagt taagtaaata ttttatgcca tctgtaatta cagtattagt attaatgttt
    13201 atagcaagac cactttgcgt tttagtttgt acacttgtag acagggaagc aaaatggagt
    13261 aaaagtgaaa tattatttat gatgtgggta agggaaacag gcgtaatacc agcagcactt
    13321 tgtggtatta tttcagcaat gaaagttcct ggatacgaag tgatttcatc tgtagtattt
    13381 atgactatac taattacatt agtaatacaa ggaagtacta caaaattagt agctaaaaaa
    13441 cttggattat tagaagaaga agtgataaat ataagtgaaa aagtatcaac tttttaaagt
    13501 tttgctttaa aatagtacct gttaatggct attttaatat tgtctaatga tgattattga
    13561 aatagtcatt tttttatgta taagtaattt acagaattta catattagac atatttatta
    13621 tcaaaatttg ttgaagctat gatataatta gttgtattta aaataagaat agcaaataag
    13681 ggaggaaatt tatgtttacg gttatggata ttgtacagcc agatacagta gaagaagcgt
    13741 actcaatttt aaataaaaga aaaactaacc aagtaattgg tggaagtgct tttttaagaa
    13801 tggggaaaaa aagaatagga acaggaatag aattatctaa attaaattta gattatataa
    13861 aagagtatga agattacgtt gagattggtt ctatgactac ttttagaaca ttagaaacaa
    13921 gttccataat aaaaaataat tttggaagaa taatagaaga ttcagttaaa aatataatag
    13981 gagttcagtt tagaaatgta gtaacagttg gtgcaacagt attttctaaa tatggttttt
    14041 ctgacttgat agtagcatta ctttcattag atacagaagt agaattatat aatattggga
    14101 gaattagtct tgaagagttt ctaaatagag attatgagaa agatttactt attaagatat
    14161 acataaagaa gacaaataag aatgcttctt ataaatcact tagaaatgca aagagtgatt
    14221 atccaattct aaatgtatct gtatcaaaac atatggaaca atttaagtta tgtgtaggag
    14281 caagaccgca gaaagctacg attgcaaaac aagctagtga atttctatct aacaatgaaa
    14341 tcaatgaaat taatatagat aaagcagtag aaatagcatc agaagaacta acttttggtt
    14401 ctaacatgag agcatctagg gagtatagaa aagctatgag taaagtactt ttaaaaagag
    14461 ctataatgga ggttatttaa tgttagtaaa gattaatata aatgggaaga aaaaaagtgt
    14521 tgatatagaa ccagaagaat atctggttga tactttaaga aaagttggta atttaagtgt
    14581 gaaaagaggt tgtgacacag gctgttgtgg tttatgtaca gttttaattg acaagaaacc
    14641 tacattatcc tgtgcaacac tgacattgag agctttagat aaagaaataa ctacaattga
    14701 aggtttagaa aatgaagtta aagaatttag tgaagttttg gtaaaagaag gagctgagca
    14761 atgtggtttc tgttctcctg gctttatact tactgtactt gcaatgaagg atgagcttga
    14821 aaatcctaca gatgatgaaa taaagcatta tcttacagga aatctatgta gatgtacagg
    14881 gtatatggga cagttaagag ctatcaaaac ttatctggag gtaaaataga acatgaatat
    14941 agttggaaaa ggaatcaaga aaatagatgg aatgagtatc acaacaggaa agcctgtata
    15001 tacagatgat ttatcagcaa aagatgcttt ggtggtaaag atacttagaa gtcctcacgc
    15061 ttatgcaaaa ataaaagata tagatacatc aaaggcagaa atggttgatg gtgttgaatg
    15121 tgttttaaca tataaagatg taccaaaatc tcgatttaca cttgctggtc aaacatatcc
    15181 agagccatct ccatatgata gattaattct tgaagatgtt gtaagatatg taggtgacga
    15241 agtagctata gtggcagcta tagatgaaaa aactgcaatt aaagcaatga atcttatcaa
    15301 agtaaagtat gaagtactta cacctgtttt ggattttgaa gaggcaatag gaaataaaac
    15361 tatggtacat gatgaagata gacattgtaa ctttgatata ggtatgcaaa gagaaaaaaa
    15421 tatagtatcc catcatgatt atacaaaagg tgatgtagaa aaagtattaa gtaattgtga
    15481 tgtagtgatt gaagaaactt attatactca agctcaagca catgcaatga tggagacgta
    15541 tagggcatac aattatttag accatatggg aagattggtt gtagtaagtt caactcaaat
    15601 accatttcat gtaaaaagac atttatctcg tgcactagaa ataccagcta gcaaaattag
    15661 ggttataaaa cctagaattg gtggcggatt tggtggtaaa caaactgcat gtgttgagat
    15721 tttttctgca attgttactt taaaaacagg gaaaccagct aaaataattt atgatagaaa
    15781 agaaactttt aattgttcaa caagtagaca tgcaatgaga ttaaaagtta gactaggtgc
    15841 tacaaatgat ggcataataa aggcaataga tataaaagca ctttctgata caggagcata
    15901 tggagagcat gcttctacaa ctattggttt ggttggagaa aaaactttac caatgtataa
    15961 taaagttgaa gcaacaagat ttattgcaga tgtggtatat actaataaga tgccagcagg
    16021 agcacttaga gggtatggag catcacaagg atgttttgct gttgaatcaa ctataaatat
    16081 gcttgctaat aaattaaaaa tggacccaac agatttaagg ttaaaaaatg tggtaacaga
    16141 aggtgaaact acatttgcat atggtaaaac tttaaatagt gtcgatttaa taaaatgtat
    16201 aaataaaggc aaagaactta ttggatggaa tgaaaaatat ccttgtaaac atcttgataa
    16261 tggaaaaatt agaagtgtgg gtatggcaat tactatgcaa ggttctggta tacaaggtct
    16321 tgataagtct tcagctgaaa taaaattgaa tgactgtgga gattatactc ttatgatagg
    16381 ttctactgat atgggtacag gaagtgatac gattttagct caaatggctg ctgaggttct
    16441 tgatacaagc attgaaaata taacagttat atcagctgat actgacttag ttccatatga
    16501 cccaggttca tatgcttcaa gtactactta tgtaacaggt atggcagtag ttaaggcagc
    16561 tcaagactta agagaaaaaa taatttctgc tggagcaact aaactaggtg ttgagaagga
    16621 tgaagtaaaa tttgatggta taaaattaac tagtaaaaaa ggtagcactt tattattcga
    16681 tatagcagtt gattctacaa ttggtccaga tactaatcaa ttaataggtc ttgcttctca
    16741 tggtagccca gtatctcctc ctccttttat agcaggtttt gcagaaactg aaattgacac
    16801 agaaactgga aaagttgatt tagtaaatta tgtagctgta gtagattgtg gaacggttat
    16861 aaataaaaat ctagctaaga tacaagttga aggtggaatt gttcaaggga ttggaatggc
    16921 attatttgaa gaagttagat acacagaaaa aggaagaatg gacacaaata catttatgca
    16981 atacaagatt ccagctagat gtgatattgg aaatgttaaa gtagattttg ttgaaactta
    17041 tgaaccaact ggaccatttg gtgctaaatc tgttggagaa gttgttataa atacaccttt
    17101 accagcaata caagaagctg tgtttaatgg aattggtgta agaataaata cactccctat
    17161 tacacctgaa aaagttttta tggaaatgaa taaataatta tttatgaaaa tttagactag
    17221 ttttacatgt taaacaacca gttatgtcat acctgttgaa agatatttaa taagtagtta
    17281 ttataatatt atgaatttta cttataaaat acaaaattaa ataaggggat gatgttttgg
    17341 ctggtaatct aaataatatg agagcagtaa ataattttag aggagataag aacattttag
    17401 aatgtttagt cagctttgag ggtcgttcaa taagtcagag aaaagtaagg gtatttttta
    17461 aagaaaaaca aaatcaaata gaaattgatt ttgcagaaga ggaaatttct aaattggttg
    17521 aaaatgttgt tttaaataca tcatatcaag aaatgttata tgatgaaata gagaaacaac
    17581 tggaaattga ttgtataggt acttggatga tattatctaa attaaaagat ggtagtagag
    17641 ttcactgatt ttaaaataaa ttattagagc ctaattttaa ggctcttttt tgctgttttt
    17701 aattaacaat agaaaactta tctttagttt gtatataatt ttaatatcat atattctata
    17761 tatataaaat tgaatttgta tgtattatat tgtataatgt aaaatgaatt ggaaaaatag
    17821 ttcgataaaa taatttgttt ttataaaatt actgtttaag tttatagatt ttgtataaaa
    17881 taaagttatg ttattaattt ttttagaaat aaaactttta ctaggagaca gatatgagaa
    17941 aaaatagtat taatatagca ctaactgcag caattacagc aatctatgca gttttaacat
    18001 tatcattggg atttataagt tatggaccaa tacaatttag ggtagcagag attatgatgc
    18061 ttttggcatt tttagataaa ggatatatag taggtcttac attaggttgt tttttagcaa
    18121 atgtaatagg accatatggt gtacctgata taatttttgg aacattagcc acatttataa
    18181 gtgcaagtat gatttatatt actagaaagt taggtaaaca gaataaaagt actttagtta
    18241 tagcttcttt atggccaact gttataaatt cgattgtggt tggtctaatg ttaaatgtat
    18301 tctttgggct tccattaatt ctatctatga tacaagtagg ttttggagaa tttgtagtag
    18361 tgacaattgt aggagtacca tttttctgct ttttaatgaa aaaatataaa aatgtcatta
    18421 atattaaatt ttaaatagtg tatagtaatt taattaattg agagtattat tagtgaaaag
    18481 aggtgtttat ttgtcgaagg taaaaagatt gactgatgtt tggatatata ttaatgataa
    18541 gcaaaagttt acaataaaag agttgtcaga ggaatttaat ctttcagcta aaacaataca
    18601 acgatattta aaagaattaa ataaaatggg acttccaatt caagcagaac aaggtagaaa
    18661 tggaggttat agggtgctaa ataatagcta tatacctcct gttatattta cagaaaaaga
    18721 agttatgatt atactatttg cacttaagtc acttcagatt tataattata agttaatgga
    18781 aatagaaatt aattctataa tgagaaaagt aagttatgaa cgtaaaagca gtataaaagt
    18841 aggtatagaa aatattaaaa gatacattgg ctttgtagct aatgatacta atgatggaga
    18901 ttttaagagt agtaatgtaa ctgagttttt tagagcttct tcagaatctc taattttaaa
    18961 tattaagtat aagtgtggta atcagatttt ggaaaaaaac gtatgcccta taggtatata
    19021 tttaaataag ggagcatggt tttcaccagc atatgattgt aaaagtgata aaataatatt
    19081 agttcatata gataatataa ttgatataaa aagaacagga gaaagtaaaa aaatacatat
    19141 aaacttggaa gaatggttac atgaagtata taacaattta tatggaaata gttcaactac
    19201 taaaaaattt ggtgaaatta tttatctaaa tgtattatta actaaagaag gtgtttctaa
    19261 aataaaggac agttcttgga atttagatat ggagctaaat gaagatggta gtggaataat
    19321 tagtacattc ataaaggttg aagatataga ttgttatgct tcgattttat tttttattgg
    19381 aaataatgct aaggttatag agccaaaatt tttgaataag ctactttatg ataaggccaa
    19441 tgagttaaga tatttttatg aagaaaatat ggtataatat ttagaaatgt tgaaattaaa
    19501 taattattaa ggagatatac gttataatgg aaaattttaa taagaatgct aaaataattg
    19561 gtattggagc tgaaggtatt aatataataa atgaagtaga agaaaaaata aaagcaaata
    19621 tggatataga aaaaataaac ataaatcaag agattgaaaa agaatatgtt agaagtcttt
    19681 tagatggtgt agatatatta tttttgatat attcatctga agataaacat attagagata
    19741 ttataaaagc tgtttcatat atgtcaaacg aaagaagagt tctatctata ggaatggatt
    19801 gttcagagaa agaaaataaa gaagatttag agcttgggag agagtttaaa atcaataatg
    19861 atagtatatt taaatttgtt gatttaatga atatcatggt agagtctatt tctgattctt
    19921 gcatgataaa tatagatatt actgatttaa aagaagctat tgtaggagat aaaggaatta
    19981 agtattcttt tgaagagttt gaagatacaa agtcatatag cgagatagct gacattttat
    20041 ttgacaggat ggaatatatt ggtgaggaat ttataagtaa gaaaggaatt gtatttgttg
    20101 aaggaagtcc agaattttct ataatggaat tgaatgattt aataagtaat attcagagca
    20161 aggtagaaga aagctatgaa gtaatatttt ctttatatat aaaggaaaat ttaaatggaa
    20221 atattagagt aggcttattg tataattagt atattttaga tattaaggag agtgtatatg
    20281 tctgaaaata taagaggtac agagtattgg ttaatggaag aattttttta tcaggaaaca
    20341 gacacaccac agatttttag agaagaaagc ataattgcac ttggatttga cataaaaata
    20401 aatttaaaaa aagtattcag tatggggaaa aaatcagaaa agtatattaa tgagaattgt
    20461 aatgataaag attttataaa aaagtttatg tcgattaaaa aaggagatta ttttttatat
    20521 aaagcatatc aaaatataga agaaaaagtt aaatgtaatg tgtctattgg catagttgaa
    20581 gaagattttg aaaatggata tgaattaagt catattttag ggcatacact accagtaaaa
    20641 tggatgcata attttgatac agtttcaaat tgtaaagtat atacaagtga attttataaa
    20701 ttaaataata atgatattag ctcatattta gaaattatta agtcacaaga taagaaatta
    20761 ttgagaaaaa gaatagaaga aagtgaaaat aataaatggt atttaaaaaa ctattattat
    20821 aaaaatgtag ataagtgtgt agatgatgaa gaattattta ttcagcccat taatctagaa
    20881 caattagaaa gcctaaaaaa tataaagaca gatgattata ttttattgta taatagcgag
    20941 aaagtaattt gtgaaaatga ccatacaatg aagagtctat ttggcgtatg tcaagttatg
    21001 gaagggttcg ataaagagaa agtaagtgct ttggaaggtg gatatttttt tcctgttaga
    21061 tggatagagt atgaaaagga gaatatagac ggaaatgcat gggttatatt tgaaaataat
    21121 agtatagttg aaaagtatgt aaaaagattt actaataaaa tgccattaaa tactatactt
    21181 tatggaccac caggaactgg taaaacatac aatacaaaac agatggttta caatctagta
    21241 agtgaaacca gtctaagaaa caaatactat acaggttact tagattataa aaacaatgta
    21301 gaattttgta catttcatca atcatatgga tatgaggaat ttatagaggg tcttaagtct
    21361 gatggtgaag gcaattttaa gcaagaagat gggatattaa aggatatatc tatagaagca
    21421 tgttataatg cattgaaatt tgaaaaaaag atgagctttg agttggaaaa agatagcttg
    21481 actttagaaa ctataaaaga caggaaaaag aaattagtat tagaaaatat agaatatagt
    21541 gagagtttta attttgaaga atcagaaaac tatgttttaa taatagatga aattaataga
    21601 gggaatgtat ctaaaatatt tggtgagcta ataacattat tggaagatga taaaagactt
    21661 actaaatcaa atcaaactat tgtcaaacta ccatattcaa aggaaaaatt ttctctacct
    21721 ccaaatttgt atatagttgg aactatgaac acatcagata aatcaatagc acttttagat
    21781 atagctttaa gaagaaggtt taattttgaa gaaattatgc cagattatga tttattgtct
    21841 attgtagatg gtatagatat tagaaaaatg ctatatacaa tcaataaaag gatagagttt
    21901 ttatatgata gagatcatgt tattggacaa gcttatctac ttgctgtaga aaatattaat
    21961 gatatagtag tagtatttag aagtaaaata ataccacttc ttcaagagta tttttattat
    22021 gattcagaaa aagtaggttt gattttagga ggaattggaa gaaatgaaaa agataaatac
    22081 attgtttata aagaaaaact tatagcttct gagttattta aaaataataa tgcctcaata
    22141 atggagagta aagttaacta ttgtataaaa caaaacatat ctagtgagga gctaaaaaat
    22201 atatatgagt aaaaatatat atgttaagga aacctatgaa tggataaggg taggtaatgg
    22261 tgaaaatgag cttacagaaa ttgaatatga aaaattgctt aaatatctag agaacaataa
    22321 tgatgtatta aaaagtaata taatagatat taaatacaaa aaacttagat ttataaacta
    22381 tgtaggcatc atatgttttg aaaatgtaat acttgaaata cttcctaaat tgtcattaag
    22441 tgataatctt gtaaaagata gagagatact attacagatg ctttctatat gtaataaaat
    22501 tccaattaca atgaatgaaa aaataagatt gagtttgaaa aattataatc tcttaaattt
    22561 ttttgttatg tatttcattg agagtatgca aacacagatg aaaaaaggta tttattttga
    22621 atatataaat aaaatagaga acttaaatgt tatgaggggt aaaatattat taagtacata
    22681 tgcaaaagaa aaaggcattt ctccaatgaa aataagatgt gaatatgatg agtatagtga
    22741 aaataatttc ttgaatcaag ttcttaaaaa agcatgtata tctattttat gtagaataaa
    22801 tgacaattca attcaaggca agattaaaaa aattttaagc tattttcaaa atgtagattt
    22861 aatttatata gatagaaaaa aactattgga ttataaattt tataaaaaca atgatagatt
    22921 taaagattgt tatttactag ctagacttat acttttaaat ttgtctatgg ataatagtca
    22981 aaataatcaa gaggcatttt ctatattatt tgaaataaat actttgtatg aagaatatat
    23041 aggaatttta atcaaaagta tatgggacaa ttcatttaga gaaacatata tacaagataa
    23101 gagtaaattt cttttgaaaa atgaacaaac aggtaagaaa aactttaatt taagaccgga
    23161 tatagtttta tatgacttaa aaaatgaata tgaaattata attgacacta aatggaaagc
    23221 tattgaagta gattcaaatg ttttttatag gtcaagtgat atataccaga tgtatgcata
    23281 tataacagca tatgaaaatg caaaaagatg tatattatta tatccttgta ttcaaaaaga
    23341 taagaattat agttcctgga aattatcaga atcatttaag ggtaagttta tagaagcaaa
    23401 aactgtgagg ttagatgata taaaaaacac aaaaaa
//
LOCUS       DAMJWU010000050        23003 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMJWU010000050 DAMJWU010000000
VERSION     DAMJWU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.050"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            284..748
                     /locus_tag="PC460_002187"
     CDS             284..748
                     /locus_tag="PC460_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2601071.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            755..1249
                     /locus_tag="PC460_002188"
     CDS             755..1249
                     /locus_tag="PC460_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2601072.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1265..2290
                     /locus_tag="PC460_002189"
     CDS             1265..2290
                     /locus_tag="PC460_002189"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2601073.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2277..4724
                     /locus_tag="PC460_002190"
     CDS             2277..4724
                     /locus_tag="PC460_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2601074.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4904..6277
                     /gene="radA"
                     /locus_tag="PC460_002191"
     CDS             4904..6277
                     /gene="radA"
                     /locus_tag="PC460_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2601075.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6280..7350
                     /gene="disA"
                     /locus_tag="PC460_002192"
     CDS             6280..7350
                     /gene="disA"
                     /locus_tag="PC460_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2601076.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7439..8533
                     /locus_tag="PC460_002193"
     CDS             7439..8533
                     /locus_tag="PC460_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2601077.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8662..10839
                     /locus_tag="PC460_002194"
     CDS             8662..10839
                     /locus_tag="PC460_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601078.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KETYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TIYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWYIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(11025..11870)
                     /locus_tag="PC460_002195"
     CDS             complement(11025..11870)
                     /locus_tag="PC460_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601079.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            12087..13253
                     /locus_tag="PC460_002196"
     CDS             12087..13253
                     /locus_tag="PC460_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2601080.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYRVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYVCGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            13275..14921
                     /locus_tag="PC460_002197"
     CDS             13275..14921
                     /locus_tag="PC460_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2601081.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            15018..16391
                     /locus_tag="PC460_002198"
     CDS             15018..16391
                     /locus_tag="PC460_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2601082.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            16836..17789
                     /locus_tag="PC460_002199"
     CDS             16836..17789
                     /locus_tag="PC460_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2601083.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17827..18789
                     /locus_tag="PC460_002200"
     CDS             17827..18789
                     /locus_tag="PC460_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2601084.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18814..19800
                     /locus_tag="PC460_002201"
     CDS             18814..19800
                     /locus_tag="PC460_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2601085.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            19813..20859
                     /locus_tag="PC460_002202"
     CDS             19813..20859
                     /locus_tag="PC460_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2601086.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            21026..22756
                     /gene="lpdA"
                     /locus_tag="PC460_002203"
     CDS             21026..22756
                     /gene="lpdA"
                     /locus_tag="PC460_002203"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2601087.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
BASE COUNT         9331 a         2386 c         4336 g         6950 t
ORIGIN      
        1 tttttattat tatttttaca aatgtttagt aataaagatg agaaggtata tttaaaatat
       61 aaagtattta aagttctgaa atctaatcat gaagcaaaaa attagcattt ggttatcaat
      121 aaatataata aataaaaact cataattaat gaaagaataa attcatgtct aaatttctat
      181 aaaaaattta aatttataca atttagggct ttaaaaattt aaataatagg tgtaaaataa
      241 tagtcaaaga aagtcaaaaa taaaaaggtg tgtggtgata ttaatggcaa ctatgacaga
      301 tataattgaa aaatttatta aagatttgat ggaagaagat aatagcatac aaattcaaag
      361 aaatgaacta gcaaacttgt ttagttgtgc accatcacaa ataaattatg tcttaacaac
      421 aagatttaca atagatagag gatattacat tgaaagtaaa aaaggtggcg gaggatatgt
      481 tcaaatagaa aaaatacgaa aaagtaaaga tgggcatatt agagaactac taaatgaaaa
      541 aataggaagt cagatatcct ataaaaaagc aaaagaatta ttagaaggtt taaaagagtc
      601 ggatttgatt aacgaaagag aattaaaatt aatattatat gcaatagatg ataaatcatt
      661 gtgtatgcca atatatgaat taaaagagaa ggtaaggtct aatattttaa agaatataat
      721 aataggatta tttagtatag aggagtgata atttatgcta tgtcaaaaat gcaataagaa
      781 taaagcatct gtatattata ataagatagt caatggagaa aaaacagaga tgtatttatg
      841 tagtgagtgt gcaaaagaga atacagaaat gaattttaac ttagatatgc ctttttcaat
      901 gatggatata ttttctaatt taggttttca acctaaaaaa gaacttgagg aaaagctagt
      961 ttgtcctaaa tgcaatacaa catatagtga gtttaagaat aatgggagat ttggatgtag
     1021 tgaatgttat aatgcattta gtagtcaagt aaatccaatg cttcaaaata tacatgggca
     1081 tatagaacat acaggaaaag cacctaaaaa atctttttat aagataagtg tagaaaatga
     1141 gattaaagaa ctaaaagaag atttagatag agctataaaa aatgaagagt atgaattagc
     1201 agctcaattt agagataaaa ttaaatattt aaaaggttct attgactaag attggagtga
     1261 tattatgaaa gagaacatag tcatgaaatc aagagttagg ctagctagaa atttaaataa
     1321 ctatccattt ccaaataaat tagataaaga atgtgccatg gaaataatag aaaaagtaaa
     1381 aaatgcgttt attaattcta acttagaaca aaaagaagaa tttgatttct ataaaattga
     1441 agatttagac caatctaaaa aaatgcttat ggttgaagaa catataataa gcccagattt
     1501 agcagaaaat gataaaagtg cagttatagt aaaaaaagat aaaactataa gtataatgat
     1561 aaatgaagaa gaccatataa ggatacaaac tatatgtgat gatttaaact tagaatatgc
     1621 atatagtgta gcaaatgaaa tagatgattt gttagaatca agtttagaat atgcctttaa
     1681 cacaaagcta ggttatctaa catcttgtcc aactaataca ggaacaggaa tgagagcttc
     1741 tgtaatgatg catttaccag cattaagtca actaggctat atggatgaac tttataaaat
     1801 ttcatcacaa ataggtatag caattagagg gatatatgga gaacgaacag aagcattggg
     1861 aaatatatat caaatatcta atcaacttac acttggaaga acagagtcta atattataga
     1921 aaatgtaagt ggacttacaa aagatgcaat ttcaaaagaa ataaaagcta gagaaatatt
     1981 gcaaaaaaaa ttaggtataa aactagaaga taaaatattt agatcgatag ggacacttga
     2041 aaattcaaga gtaatgagta gcgcagaggc aatgagccat ctatcaaata taaaaatggg
     2101 aatagaaatg aactatatag ataaactaga tttaagggct atagaacaac ttatgatagg
     2161 tatacaacca gcgcatcagt caataatgta taagagtgat gatgttgaga atagagatat
     2221 aaatagagca acatatatta gagaaacttt ggaaaaatta agaggaggga tgaattgtga
     2281 acttcaatag atttacacaa agagctaaaa aggcaattga tttagcgttt gaatctgcta
     2341 aaagtctagg acacaatatt gtcggaagtg aacatatact tttaggtctt ttaagagaag
     2401 aagaaggtat agctgctaaa gttctaagta aagttggatt tacagaagct tatttagaag
     2461 gcaaaatagt tgatatggaa ggtaagggag aagaaatctc agaagatata gtattaagtc
     2521 caagaagtaa acaaatactt gaattatcag gaatgtttgc aaataaatta aaaacaaatt
     2581 atattgggac tgagcatata ttattagcaa ttattcaaga aggtgaagga atagctaata
     2641 agattttaaa ttatgctgga gtaaatgata ggactttagc ccaattaaca attgatatga
     2701 tgggtattag tgataaaaat caatacaaag cagaaaatag ttacacgggc aaccagaatc
     2761 aagcagaatc taaagtactt gataagtatg gaagaaatct tacactatat gcaaaacaga
     2821 ataaaataga ccctgtaata ggtagagaaa aagaaataca aagagtaata caaatattaa
     2881 gtagaagaac taaaaacaat ccagtgttga taggagaccc aggtgtaggg aaaacagcca
     2941 tagctgaagg tctggctaca aatatagcct taggaaatgt tccagaaaca ctaaaaaaca
     3001 aaactttata ctctttagag atgggttcat tattggctgg ggctaagtat agaggagaat
     3061 ttgaagaaag aattaaagaa gttgtagatg aagttgttaa aaatggaaac ataatcttat
     3121 ttatagatga gatgcacaca ataatagggg ctgggtctac tggagaaggc tctatagatg
     3181 cttcaaatat attaaagcca gccttagcaa gaggagaaat acaagttata ggtgcaacta
     3241 caattgatga atatagaaag catgttgaaa aagattctgc tctagaaaga agattccaac
     3301 cagttatggt agatgaacca actaaagagg actcaataaa aatattagaa ggattaagag
     3361 ataaatatga agctcatcat aaagttaaaa taactgatga tgctataaag gcagctgtag
     3421 aattatcaac tagatatata tcagacagat atctgccaga taaagctata gacttgattg
     3481 atgaagctgc atcaaaagta agattaaaag aaaatacacc tccttcagaa ataaaaaaat
     3541 tagaactaga aatagaaaat atagataaag aaaaagaaga agcagtaaga tgtcaagact
     3601 ttgagaaagc agccaaaata agagatgaac aaggtctact taaaaaacaa ttagaagatg
     3661 ttagagaaag atggaacaag tcatctaaac attcagattt agttgatgga gaagttattg
     3721 ctgaagtagt gggcttatgg acaggaatac cagttaataa aattcttgaa gaagaggctg
     3781 atagactttt gagacttgag gaaatactgc acaatagagt tataggtcaa gaacaagcag
     3841 taaaatctat ttcaaaggca atcagaaggt caagagcagg tcttaaagac cctaatagac
     3901 caattggttc atttttattt ttaggtccaa caggagtagg taaaacagaa ttatctaagg
     3961 cattagcaga agtgcaattt ggagatgaaa atcaaataat tagaatagat atgtctgaat
     4021 atatggaaaa acatgctgtg tcaagaatga taggttcacc tccaggttat gtaggtcatg
     4081 atgaaggtgg tcaattaact gaaaaagtaa gaagaaatcc atattcagtt atattatttg
     4141 atgagataga aaaagctcat ccagatgtat ttaatattct gttacaaatt ctagatgatg
     4201 gtagactaac tgattcaaaa ggaagaactg tagattttaa gaatactatt gtaataatga
     4261 catcaaatgt tggagcatct acaattggta gacaaaaaac tttaggattt agtatagcta
     4321 aaggagacga agaagaaaaa tctcaatatg aaaaaatgaa agaaaatata atgggtgaat
     4381 taaaacaaag attcagacca gagtttttaa acagaataga tgatataatt gttttccact
     4441 cactaaatga aaatcatata tcaaaaatag tacttttaat ggcagcgaaa ctacaagaga
     4501 gattaaaaga aatggatata aaattagaaa tgagtgatga ggctgttaaa ttaatttcta
     4561 aatctggatt tgacttagaa tatggagcaa gacctcttaa aagagcttta caaaaagagt
     4621 tggaagatga gctatctgaa gcaatcttaa aaggtaatgt gaaaaaggga agtaatgtag
     4681 tagcaaaagt caaagatgaa aaaatagttt ttgaaactaa ataatttaat agagttgcat
     4741 tttaaaatat tgcaaatata agttttataa tctaaacaaa attttaaggg caatgaatct
     4801 ataataattc attgtcctta attattttat ttaatcttta catattgtct ttcagatatg
     4861 ctaaaattat agtatagaat aaaaaattaa ggatgataaa agtatggcaa aaataaaaac
     4921 aaaatatgta tgtcaatctt gcggatatga aacagctaaa tggcttggta aatgtccaga
     4981 atgtacaaaa tggaatacat ttgtagaaga aatagaccaa aaaagcacca aaaaagaggt
     5041 ctttataata gataaatctt catctaaacc agtcagtata aactcaatag aaagtaaaga
     5101 agaagagcgg tttacaacag atataaatga gttagataga gtgcttggag gaggtatagt
     5161 taaaggctct ttagtgcttg ttggtggtga ccctggtata ggtaaatcaa cacttttaat
     5221 ccaagtatct agtaatgttg ccaatttagg aaaaactgtt ttatatataa ctggagaaga
     5281 atcagaatct caaataaaaa tgagagctaa aagattaggt attaactcag aaaatttata
     5341 tatatttgct gaaaataatt taagtataat tgaatcttat ttagaaagtg taaatccaga
     5401 attaataatt ctagactcaa ttcaaactgt cttcagtcca gaaatatcct cggcaccagg
     5461 aactgtcagc caaattaaag aaggtacttc aaaattcatg aaaatttcta aaaaaatggg
     5521 aatttcaacg ttcatagttg ggcatgttac aaaagaaggt tcactagcag gaccaaaatt
     5581 attagagcat atggttgata ctgttctata ttttgaagga gaaagatata atacttatag
     5641 attagtaaga gctgttaaaa atcgatttgg ttcaactaat gaacttggag tatttgaaat
     5701 gagggattta ggattagtag aacttgataa tccatctaaa attttaatct ctgaaaaacc
     5761 aaaagatgta gcaggttcag taataatatc tactgtagaa ggtacgagac caatgttact
     5821 tgaattgcaa gcattagttt ctcctacaag ttttggtata ccaaaaagaa catctacagg
     5881 agtcgattac aatagagtag gtatgttatt agcagtatta gaaaaacgtg ttggtttgca
     5941 aatacaaaat caagatgtct atataaacat agttggagga ataaagataa atgaaccctc
     6001 aattgattta gggatagcca tttctgtagc atctagtttt agaaatatac cgatagatga
     6061 agatatagct gttacaggag aagtgggact tacaggagaa gttagagcag taagctttat
     6121 agaaaagaga atagctgagt gtaaaaaact tggatttaaa aagatagtag ttcctagaag
     6181 taattatgat gttgtaaaag agaccaaagg catagaaata tggccagtag ataacttaag
     6241 acaagctata aatatagtac ttgggaggaa tcaataaata tggagaattt tctagataat
     6301 aaaaatatgc tatatgcatt aaaaatgata tctcctggaa ctccacttag attaggtcta
     6361 aacaatgtac taagagctaa gactggtgga ttaattgtaa ttgcaacaaa cgaagatgta
     6421 atgaaaatag tagatggagg atttgctata aatgcagaat attcaccatc atatctatat
     6481 gaattagcta aaatggatgg agctatagtt ttaagtggtg atgtaaagaa aatattattt
     6541 gctaatgcac aacttatacc tgactatttt atagaaacat cagagacagg aacaagacat
     6601 agaacagcag aaagagtagc aaaacaaact ggtgctatag tcataggaat ttcacaaaga
     6661 agaaatgtta taacagttta tagaggaaat gagaagtatg tagtcgaaga tatatctaag
     6721 atatttacta aggcaaatca ggctatacaa actctggaaa aatataagac agtattggac
     6781 caagctgtaa caaatttaaa tgccttagag tttaatgatt tggtaactat ttatgatgtt
     6841 gcattagtca tgcaaaagat ggaaatggta atgagagtta caagtataat tgaaaaatat
     6901 gtgatagaat tgggtgatga aggaacttta gtaagtatgc aattagaaga attaatgggt
     6961 acaaccagaa tagaccagaa attaatattc aaagattata ataaagaaaa cacagaaata
     7021 aaagaactta tgaaaaaggt caaaaattta aattcagaag aactaataga attggtcaat
     7081 atggcaaaac tattagggta tagtggtttt tcagaaagta tggatatgcc tataaaaaca
     7141 agaggttata ggattcttag caaaatacat agactaccaa cagcaataat agaaaactta
     7201 gtaaattatt ttgaaaactt tcaacaaatt ttagatgcat ctattgaaga attagatgag
     7261 gttgaaggaa taggtgaaat aagagcaaca tatataaaaa atggactcat aaaaatgaaa
     7321 caattagtct tattagatag acacatatga tgaattttaa atgaatttta ctactttttt
     7381 cttgaatata atatacactt tggtctataa tagataagta taactagggg gtgaagcatt
     7441 gataaggaaa gtaacaagaa tattgtttat tttacttgga tttacaatag gtataacaac
     7501 ctatttaaca ttaatgaaag attttgaaat attaacattt ggaaaagaaa catatggtta
     7561 tatcgcagct gtagtagcag gtataataat agccatttta ggatatctta tagaaccatg
     7621 ggttgtaaat aaagtaaaag aaattgctaa aatagtggat aaagaattgt caaaatatcc
     7681 tcagactgat atattgttag ggtctatggg tcttatagta ggatttgtta ttgcgtattt
     7741 attaagtgga ttagtaaata gaataccaat agtaggtgga atactatcct tactattata
     7801 tttattccta ggatatcttg gaatgaaagt tgcgcttaaa agtaaaaatg atttatttaa
     7861 tattggaaaa ctaggaagat tagcaaatcc tattaaggat aaagataagg ataaagaaaa
     7921 taaaaaagaa gtaaaagcca ttcctccaaa agtattagat acaagtgtaa taattgatgg
     7981 aagaatagct gatatttgta agactgggtt tatagaagga aaacttatta ttcctgcttt
     8041 tgttttggag gaactaagac atattgcaga ttcttcagat gatttaaaaa gagtaagagg
     8101 tagaagaggt ctggatatat taaatataat tcagaaagaa ttaaatatag aagttgaaat
     8161 aagtgagaga gactttgatg atatagcaga agttgatagt aaattattaa agcttgctca
     8221 ggttttaaat ggaaaagttg ttacaaatga ttataactta aataaagttg ctcaattcca
     8281 aggtgttgaa gtattaaata taaatgagtt agctaatgct ataaaaccag ttgctatacc
     8341 aggtgaagat atggttgttc aagtggttaa agaaggaaaa gaggctcaac aaggtgttgc
     8401 atatctagat gatggaacta tgatagttgt tgatggagga agaaaatata tgaatgagac
     8461 tataaaagta ctcgttactt ctgttcttca aactcctgca ggtagaatga tatttgcaaa
     8521 acctaaaaat taatatgttc aaagaaacct ataatttggg ctgtttattt taaatagatg
     8581 gtccaaattt atttatttat aacgataatg tttaatgtat tcttaaagat ggggatttaa
     8641 atcagaaagc agggagaatt tatgagaaaa aagattatat ctttcttagc aacatttatc
     8701 ataatattaa catcagcaag tcagtatagt tttgcagatg atatatcaac acgtggaaaa
     8761 gtaatattta tagatatgaa tagaactagt atgagtaata tgttaagaat aaaatcatta
     8821 agagaagaac tagacaatag agggtatatt ggcttgatga atataagagg cgataaagga
     8881 agtgatgata gaaggtcata tgctagtatg ggtgctggtg gtagagctaa tgtggctaat
     8941 gaagaagata ttaattttga aagctcgagt aaggatagaa atatagtttt tgaatcagct
     9001 acaggaaaaa gtgcaaaagg tataaataat ttaactataa ataagtctat aaatgaaaat
     9061 ttaaactttg gagagtatgg ttctgtatta gggtcattag gacaaagttt atctgataat
     9121 ggattaaaag catctgtatt gggtaattca gatataattg aaaatggtca acttataaaa
     9181 aatagaaact tatgtctaac tgcaatggat gaatatggaa gaatacctaa tggaaatgta
     9241 gatactataa ataaaaaaga cttatctatg ccttatggaa tatcaactga ttatgataaa
     9301 ttgattgtag agactaaaga aacttacaaa aataatgatg tgatttttgt ggagcttgga
     9361 gacacatata gattagattt atataaacca aatttaaatg aaaaaacata tgagagtatg
     9421 aaagataaca tagaaaaaaa tatagatata tatttaaaaa atatattttc tatggttgaa
     9481 gaaaatgaca ctatctacat agcaagtgca tttccaagtg atttagatta taaaaataaa
     9541 agaagacttt ctccaataat aaaattaaat ggagaaggga aaggtatatt atcatcttca
     9601 actactagaa gagaaggtat tgtaacaaat ttagatgttg gggttgaaat attagacaac
     9661 tttaatataa aaaatcaaaa tatggttgga agaaaatatg aacttattaa cagagatgat
     9721 aataaagaat ttttgatgga tgaatatcaa aagatagtat ctatttcaag cataaggtct
     9781 acaatactga atggttttgt aagtatagtt ttcttgtcat ggatagttgc tatgattgca
     9841 attttattta gaaagcatat atcaaaaaat tataaagaaa caacattttt tatattaaag
     9901 gaacttttaa aaataggaat agtaatgcca ctgtcattta tgataacacc aataatgaat
     9961 ttcaaaacac cattagcaat atctttagga ataattataa taacacttac tatatatctt
    10021 ataagtaaag ttttaataaa gaacgattta aaaaacatgc tgttttttac tggattaacc
    10081 atagtaatta tggttattga tgctgggttt ggctcatatt taatgaaaag caatgttatg
    10141 agttatgatt gtataattgg agctagatat tatggagtag gaaatgagta tcaaggtgtg
    10201 gcaatcggta gtgcaatatt tacatttgca attttactta catataaaaa tataccaaag
    10261 tggtatataa tagttttttc tttggttatc ttaattacaa gtgcatctcc aattatggga
    10321 gcaaatgttg gaagtgctat ttctgaatgt gttgcatttt tactatttat actattgata
    10381 tataatgtaa agatagattt caaaaaaatc gtattattag gtatagcagt agtatttgta
    10441 ttaggagttt ttgtagcaat agatatgatt ttgggaagta attcacattt aggtatgttt
    10501 gtaaaagaaa tttactttaa tggaccagga gaaataatac aaacatttag tagaaagata
    10561 gagatgaatt taaagctagc tcaaaccagt gcatgggtaa atatactgtt aactggaata
    10621 ggtgtaattt tagttcttat gattaatcag attagatact ttaagcaact gatggatgag
    10681 tatccaattg tatttaaagg atttatagca tcaatagcag gatgtttagt aactctttta
    10741 gtgaatgact ctgggattgt gtcatcagct acagccttta tttatgtaat tgtacctatg
    10801 ataacattaa gtataaatct tacagcatta aaagaataag tactgtatga ataatatgta
    10861 aatattaata cattaattta acttctttat ttacaaaata aagaagaaag tttataaaaa
    10921 ataaattata ataaaaaaga gtttatctca aaattgatta ttctaatcaa attttaagat
    10981 aaactttttt tattataaag ataaataaca tataacctca tattttaggt agattttttt
    11041 cttatatgtt taggagtgca tttaaatttt tccttaaaaa ttctgtaaaa taattttgta
    11101 ttagtaaatc catgagcttc taatatactg cataaggtat catttgtgtt aattaaatct
    11161 ttataaatat gagaaatacg cacatcattt aaatactcta agtaagtttg tcccatatac
    11221 tgtttaaatt ttctacaaaa atattctggt tgtaagtgtg ctaccttagc gattttgtta
    11281 atagaaatag gcattttgta attaatcttt gtatattcta aaactggttc aaattttgac
    11341 aaatcaagta ctgttttttt catattttta ttacctacag atattttaaa gtttgtatat
    11401 aattcaaata aaagttcaaa taataagctt gtaaagagca gattagctgc aggaggagaa
    11461 ctaagtttaa tttctctcat gttttctaat ataagtttaa ctttacttga atttttttta
    11521 tagttattgt cattggcatt aaccttaaag tcaaaatagc aatttttaaa atcagggata
    11581 tatttgttta gaaaagacaa tggtacttgc aataatatag aggtattttg ataaatacat
    11641 ctagttgagt gaataattcc agaattaatg aacatacact cattagcttt taaattataa
    11701 gtgtaaccct ccatgttaat ttgtaaatca ccagaagtta tataaattat ttctaatgag
    11761 ttatgccaat gatttgctac ataactacca ctatcatcaa atagtttgaa attaacatct
    11821 aaattatcaa taggttcaat tatttcatgt gaaaaattat ttctgttcat aataatatcc
    11881 tccttataat tttaaattat aaatagtaaa tttataatgt tcaatattaa aaagataata
    11941 tagactaata aattagataa taaacatact taagaaaacg gataaaatca aatataataa
    12001 ctgtatatta aaataactta aaaaaattat tttattataa gtgcaaaata tacctattta
    12061 aataagataa gaggggagga ttttatgtgt atataaaagg tgtaaattta ggaggttggt
    12121 tagttttaga aaaatggatg acctcaagtt tatttgaagg cacagaagct gaagatgaat
    12181 actatttacc aagacagctt tcaagagagg catatgaatc tagaattaag actcatcgta
    12241 gtgaatacat cacagagaga gattttgcaa cgattaaatc tatgggattt aattcagttc
    12301 gtataccagt accatacttt atttttggag attgtgaacc attcatagga tgtgttaaag
    12361 aacttgataa agcatttgct tgggcagata agtatgggct tagtatattg atagacttac
    12421 atacagttcc aggaagccaa aatggatttg ataatggagg aatatcaggt atatgtagct
    12481 ggtcacaaaa tccagaatat gtagcgttta cattaaatgt attagaaaga cttgcaaaac
    12541 gttatgggat gaggcatgaa ttgtatggaa tccaaatttt aaatgagcct ataacagaaa
    12601 gaatgtggaa cataatgaat gttccaaatc ggtttaaggc tgtagataag gagatggcaa
    12661 gggggagtaa acctaattca ttagagtttt tacgtgattt ttatataaaa gcatataggg
    12721 taatgcgtcc ttatatgaga gaggaaaacg ttatagtgtt tcatgatgca tttgaattaa
    12781 aagcatggaa agactttatg agagaggaag aatttaaaaa tgttgttcta gatacacacc
    12841 aatatttgat gttagctgaa gcagatggat gtgaacaaag tattgatagc tatctcaaat
    12901 atattagaga aaattatgct aaagatattt tacaaatgca aaaatatttt cctgttatat
    12961 gtggggagtg gagcttattt aattcatatg tttgtggtat agatacaaat ggaggtcaat
    13021 cacctctaaa tgggattgaa tcaaatatag ataaattatc aaaagacgat aaaagagaat
    13081 tatataggaa gatagcaaaa gcacagcttg atgcatggag aaatggaagt ggtcactact
    13141 attggaatta taagttatta ctagatacag ttaatgaaga aggttggata ggatgggata
    13201 gttgggacct aggcaaatgt gtagctcaag aatggtatcc tatagaatat taaactaaaa
    13261 ttggaggaaa agatatgatt aaaaacccta tacttccagg ttttaatcca gatccttgta
    13321 tttgtagaaa gggagatgat tattatcttg tagtttcatc ttttgaatgg tttccaggta
    13381 tacctgttta tcactctaaa gacttaaaaa attgggagtt atatactcat attttgacag
    13441 atgaaacaaa aatagatttg aagaagcttc cttcatctaa aggaatttgg gcaccctgtt
    13501 tgacttattg tgaagaagaa gatttatttt acatagttta tggaattatg aactctatga
    13561 atgcaagata ttttgatgtt gataattatt taattacatc aaaagatata aagggagagt
    13621 ggagtgaacc agtatatcta cactcatcag gttttgatgc gtctattttt catgatgatg
    13681 atggtaaaaa gtggattgca tcattagact gggagactag agaaggatat gaaaaacctg
    13741 gtgttatatg tttagtggaa tactgtacaa aaaagaaaga aatagtagga tatccaaaga
    13801 gaatatggtc agggggaact gatagaggtt gtattgaagc accacatata acaaagcgtg
    13861 gagattacta ttatataatg tgtgcagaag gtggaactgg atatggtcat agtgtaacta
    13921 tgggaagggc taaaaatata tggggacctt atgaaaaaga ttcaatgaat ccaattgtaa
    13981 cttcaattcc aggcgatttt tatgaaagac atgaccctga ccatctaaag cctaaatact
    14041 ataatccaga atcaaaactt caaaaatctg gtcatggaag ctatatagaa acaacatcag
    14101 gtgaagtata tttagttcat ctaacatcta gaccatttgt accagagtta aggtgtactt
    14161 taggtagaga aacagctatt caaaaaatga aatggacaaa ggataattgg cttcgaatgg
    14221 aagatgaaag taatttggca aaagaatacg tatcagaaag taaactagaa gaacatttag
    14281 tttcatctat accaagtttt gatgactttg attcaaatga attaggttta cagtactatg
    14341 ctcctaggat ttcaccatta tcatttgcag atgttaagtc tagaccagga tatgtaagga
    14401 ttagaggaca agaatcaaga acatcattaa ataaagtaag catattggca agaaaattaa
    14461 cttcagtata tgcaagaata accactaaga tggagtttta cccagaggta catcagcata
    14521 gtgcgggttt gataatgtat tatgataata tgaattatat aaatttaaga aagtattata
    14581 gtgaaacatt gggtcaaagt gcactatcaa taatacatct tgaaaatgga gagaaaacgg
    14641 aatttttaaa tactaggata ccaataaaag atataccaat atatcttcgt ctgtatatac
    14701 aaggtcgtaa gtcatatttt gaatggagtt atgatgaaaa aaactaccag agaataggaa
    14761 aggtatttga tacaactaag ttttcagatg agtactgtaa atatggagaa tttacaggta
    14821 cgtttatagg attaacttgt gcagatcgtg taaagcataa acactatgct gattttgatt
    14881 tttttgaata tatagtagat gaatcaaaag atgtcgatta ataaaatcat tactaaaatt
    14941 ttcagagcaa aggtaaatct tatgctctga aataagaaac attagaaggc aaatcttaca
    15001 aaaaggagta tggtattatg aatacagaag ttaatgttaa agcaactacg attagatatg
    15061 aaaagatttc attttttgaa agaatttctt atggttgtgg ggatttgggg tgcaatatca
    15121 tatactcagc tatgtcagca tttttgctgt tttattatac taattatgca gatgttagtg
    15181 cagcagctgt aggtagtatt atgcttgtat ctaggatact ggatgggttt agtgacttaa
    15241 caatgggaat tatagtggat agaactaaat ccaaatacgg taaggctagg ccttggattt
    15301 tgagaatggc gattcctttt gctatagcag cagtattatt attttctgtt ccatcaaatc
    15361 ttggtatcac ttctaaatta atttatattt ttattacata taacttagtt tctactgtta
    15421 tttatacagc aatcaatgta ccctatgcaa ctctaaattc acttataact caaaaccagt
    15481 atgaaagagg agtattaagt atatttagaa tgatattagc cacttgtggt accttactaa
    15541 taaatggact tacactacct ctagttgaat actttggaaa taatttatca gcatggacta
    15601 aaacattctt tgtatttgga atagcatcta taatggtatt ttttataaca tttactggaa
    15661 ccaaggaaag agtaaaagca gttaagcaag ataggaatga agtaatacca tttaaaatag
    15721 gtattaaatc tttatttaga aataagtact ggatacaaat aacattatgt ttagtttgca
    15781 tatttattgt atttgcgata aatggtggtt catccgtata ttatgcaaag tttattttgg
    15841 gagatgaaaa attatttggt cctataaata tggtttcaaa tatatcccaa attatagcta
    15901 tgtttatggt tgctcctttt ataaaaaaat ttggaaaaag aaatgtatta atagtaggtt
    15961 caataatatt aatatcgtca aatgttatgt ttataatagt tggacaaaat catataggag
    16021 taatatttgc aagtgctata aagggaatag gaagtgctgg aatagcatca acaatgtttg
    16081 ctattgtttc agatactatt gaatatggag aatggaagac tggatataga actgaggggc
    16141 ttataaatag tgcatctagt tttgggttta aagtgggaaa tggattaggt tcagcaatat
    16201 taggtgcagt attatcaatt ggtggttatg taggaacatc agctactcaa agtgatttag
    16261 ctatagtatc aataaaagta tgttttatat acctacctat atttataaca atattacaaa
    16321 ttatcataat gtctttttat aagcttgata aagaatatag tactatactt aatgaattaa
    16381 atactaaata atgaaaaagt ttcttataaa gtcttaagtt taattaaggt ctattgtaaa
    16441 tgttccctat aaaaactatt atatatttat gattattgaa gaatttttag agaagcgaat
    16501 aataaaaata taagaagact ttaaatgatg aaatggttgg ttttttagaa atgatttaat
    16561 aagttattgt tatttaaagc attataagtg gacaattaat ttttaattta tggagatgat
    16621 atttaacaat tagaagtaat atataatatg cactaagaag caatttttca actatttatg
    16681 aaggtggtgg tacaagaaaa gtatcacctt ttttttgtta tacaaaaaaa cagttgactt
    16741 tttatatgaa ccccttctat aatgttactg aaattaagta aaatgattga acaaatgtaa
    16801 agataattga aaagcttgat tttggaggaa agcaaatgaa aaaaattgga gacttaagat
    16861 taatgatgaa gtgttgttca ctttattacg aagataattt gaatcaacag gaaatagcaa
    16921 atcaattggg aatatcaaga ccaacaatat caagaatatt aaaagaagct tttgaacaag
    16981 gaatagtaaa aatacagata gttgatgttt tgaaaaatga ttaccaaaaa atagagagaa
    17041 gtttggagag aaaatataaa ttaaaagaag taatagtagt agatgataaa caggatgcac
    17101 taacacaaaa acaagaacta gcaagagctg tatctgaata tttaactaga gtagtaaagg
    17161 aaaatgatat aataggtgtt tcaataggaa caacactaaa agaaatacca agatatgtgg
    17221 aaaaaagtaa ctgtaaaaat gttacattta tacctctatt aggagggatt ggagacaatg
    17281 aaatagatat tcatgcaaat caaatagccg taagtttagc gagggctttt ggaggtgatt
    17341 ttaaattatt acatgctcca gctgttatgt ctgacttgtc tacaaaagag aaactttgca
    17401 aagacgaaaa aattaaagaa gtactagatt taatagataa aacaactata gcaattgtgg
    17461 gtataggaaa tcctatgagt ttaaactcaa caataatggc ttctggatac atgaatgaag
    17521 cagatataga agatttaaaa aaatacaact caataggtgc aatttgttta caagcctttg
    17581 ataaagaagg aaaaacttct atacttgaat ttaatcaaag agtattagga gtgaaactgg
    17641 aagatttaaa aaagataaaa agaacaatag gtgtcgcttc aggagatgaa aaaatagagg
    17701 caataaaggc atcattaaaa gcgaaattta taaatagttt agcaattaat cacagtttag
    17761 cacttaaatt attagaagat tgtgactaat agaaagatag ataaaagtta acaggaggga
    17821 atgtatatgt caatatcaaa agaaacattg cttgaaatgt ataagagaat gaatcaggct
    17881 agaaaatttg aagagaaagt tagttggttt tttgcaagag gtatggttca tggaacaact
    17941 cacttatctg taggtcaaga agcgtcaagt gtggcggctg taatggcttt agaaaaagga
    18001 gatttagtat cattaactca tagaggacat agtcaattta taggaatggg aatagaccta
    18061 aataaaatga tggctgaact tatgggtaaa gaaactggat tttgtaaggg aaaaggaggc
    18121 tcaatgcata tagcagatat tgagtcaggt aacttaggag caaatggagt tgtaggtgga
    18181 ggtcttacaa tagctccagg agctgcactt acacaacagt ataaaaaaac aggcaaaata
    18241 gttttatgta gttttggtga tggagcatca aatgaaggta cttttcatga aggaataaat
    18301 ttatcttcaa tatggaaatt gccaataata ttttactgtg agaataattt atacggtatg
    18361 tcaacaagca taaaacgtca tatgaatata gaaagtattg ccactagagc agcttcttat
    18421 ggaatagaag gaatatcaat agatggatat aacccaatag aagtatacga aacagtacaa
    18481 aaagctgctg agaagtgtag aagaggagaa ggacctgtat taattgaaag tagaacttat
    18541 agatggttag gtcattcaaa atcagatgca aatgtatata gaacaaaaga agaaatagaa
    18601 tcatggaaag caaaagaccc tattgaattt ttaaaaaatt atcttataga aaataatcta
    18661 tctaatgaag atgaattaga taagattcaa gaatttgcaa agcaatcaat agaagatgca
    18721 gtagagtttg ctcaaaatag cccaaatcca aaaatagagt ctttattaga ggatgtatat
    18781 gcagactaag aatggtaaag gaggaaaata aaaatgagta caagggaatt aacatatgca
    18841 caagcaataa aggaagcaat gtcagaagaa atgagaagag atgaaaatgt aatatttatg
    18901 ggagaagata taggaatata tggaggagca tttggtgtat cagttggtat gatagatgaa
    18961 tttggaccag aaagagtaag ggatactcct atatcagaag cagctatagc aggagcggca
    19021 gcaggtgcag cagcaactgg cttaagacct attatggaag taatgtttat ggactttgta
    19081 acaatttcta tggatgcaat tgtaaatcaa gcggcaaaaa tgagatatat gtttggagga
    19141 aaagcacaag ttccaatggt tgttagatgc ccaggaggtt ctggaactgg ttcagcagag
    19201 caacattcac aaagtttaga agcttggttt tgtcatgtac caggagtaaa agtagttgca
    19261 ccttctacac cagcagatgc taagggattg ttgaaagcag ctataagaga taataatcca
    19321 gtcatatttg ttgagaataa attgttatac agaaaaaaag gttttgtgcc agaagatgat
    19381 tatgtaattg aaattggaaa ggcagatatt aaaagagagg gtactgatgt tacagtaatt
    19441 acttatggaa gaatgttaca aagtgttgaa gaagcagcag agactctatc aaaagaaaat
    19501 ataaatgttg aaattataga tttaagaact ttatatccat tagataaaga gactatagta
    19561 aagagtgtat gtaaaactgg gagagtttta atttgccatg aagcagctaa gactggtggg
    19621 ctaggtggag aaatatcagc cctgataaca gaaagtgaat catttgatta tttagatgct
    19681 ccagtaaaaa gaatttgtgg aaaagatgtg ccaataccat ataatccaga gttagaaaaa
    19741 gcagttgtcc caagagttga tgaaatagaa gaagcaataa aatctttaat tgttagataa
    19801 ggagggatag atatggtggc aaataaaata aaagccacac cagcagctag aagtcaggca
    19861 agaaaagaca atataaaatt agataggctg attggaagtg gagaaaatgg aaggattcat
    19921 ttagtagatg tgttaaatta tttaaaagat aataaagcaa atactactcc tttagctaga
    19981 agaatagcag aagatttaaa tatagattta gaaactattg taggtactgg atataatgga
    20041 aagataagaa agtgtgatgt ggaaaaatta actggaaaag aaactattgt atcaactaat
    20101 acatctaaat ctagtgagaa aaaagagctg aaaattgaaa atgaaaattc aagaatgttt
    20161 aacacagtag aaggaatatt tgaaaagcca aatccaatga gagctactgt agcaaaacga
    20221 atgtcagaaa gttattttag tgctcctgta tttacattca atatagaagt agatgctaca
    20281 gaattgaagg tacttagagc aaagttaatc gacacagtaa aagaatcaac tggagtaaaa
    20341 ttaactatga cagatttaat agtaatggca gtatctaaaa tacttccaaa tcatcaagcc
    20401 ttaaattcag catggacaga tgagggtata tttagatata aagatgtaaa tatagctatt
    20461 gcagttggtt tagatgaagg cttatatgtt ccggtagtaa aaaatgcaaa caaaaagtct
    20521 ttaaaagaaa tagcaaaaga aagcaaagaa cttgcggaaa aagtaaagac agggaaactt
    20581 atgccagcag accaagaagg taatacattt acaataagta atgttggaat gtatggtata
    20641 actacattta cacctataat aaatatgcct tcaagtgcaa tcttaggggt aggtgctaca
    20701 caagataagt ttgttccagt aaatggagag gctaaaataa aacctataat gaacttatca
    20761 ttaacatcag accatagagt aatagatgga acagtagctg caaaattctt aaaagattta
    20821 aaagaattgt tagaaaatcc tttatcaatg cttgtatagg ttttgatatt ttgttactga
    20881 tataaatagt ttgaatagat tatattatta taattgtttc agtaaattta aagcttttat
    20941 atattaagaa ttatttttaa cagtaactat atgtgaatat gatttgctta attaaaatat
    21001 atgaaaataa ataggaggag agcctatgtc agtagaagta ataatgccaa aagcaggagt
    21061 agccatggaa gaaggtacta tagtatcttg gctaaagcaa gaaggcgaag aagtaaagat
    21121 tggagaacct atactagaaa taacaacaga taaagttaat atggagatag agtcagaagg
    21181 agaaggtaca cttgctgtaa tcatacataa agaggaaggt gaagtattac cagtatttac
    21241 agtaataggt gtaatagcag aaaaagggga aaatcaagag gaagtaaaag ctaaatattt
    21301 atctggaaat gtttcaaaag aagatatagt tgaagagaat caaaatatag aatttaaaga
    21361 agaaaaaata aacaaaaaag agtgtaatca tgattatgat gtagtggtaa taggaggagg
    21421 acctggggga tatttatcag cattaaaagc agctctttta ggtggaagag ttgctttagt
    21481 tgaagaaaat atactaggag gtacttgctt aaatagaggt tgtatcccta caaagactta
    21541 tataaaaaca gcagaaatat tagaagaaat tgaccaatta tcaaagagag gtgtaaaagt
    21601 aactgtagat aaagaacagg acattaagaa ggccataaaa tataaaaata gagtagttaa
    21661 aaaacttaca gctggtgttg gcggattatt aaaaagtaga gatgtagatg tgtttaatct
    21721 taaagctagt gttaaagaag agcataaagt aattttatca gatggaaaag ttttagatac
    21781 agaaaatata ataattgcta caggttccaa agtaaggata ttacctataa aaggaataga
    21841 atcaaattta ataataacta gtacagaagc attagatttg gaaacagtgc cagaagaatt
    21901 agtaataatt ggtggtggag tgattggttg tgaatttgca gagatattca attcaagagg
    21961 ttctaaagta actatagtag aaatggaaga tagagtaatc ccaagaatgg ataaagagtt
    22021 aagcgaatct ttaaaatatt ctcttagtaa aaaaggtata aatgtattaa ctaaaaagaa
    22081 agtttctgaa tttaaagaag aaggaaataa catattagtg tgcattgaag gtgaagagcc
    22141 aataaaagca gatttatgtc tatatgctat aggaagagaa gctaatcttt ctggcataga
    22201 ggacttagat attaaaatag ataaaggttc aatagttgta aatagtaaaa tggaaacaag
    22261 tataccaagc atatatgcag taggtgatgt tactggaggt gtaatgttag ctcatgctgc
    22321 ttttaaaatg ggagaggtag cagcttcaaa tgctctaggt gtgaataaag aagttgactt
    22381 aggtgcatta cctagttgtg tttatacaat tccagaagta gcatcagttg gtattacaga
    22441 agaggatgct agaaaaaaat ataatgtaaa agttggtaag tttaattttg ctggaaatgg
    22501 aagagcttta gcttctggtc aagaacaagg atatgtgaag gtagtagcag atgctaaata
    22561 tggagaaatt ttaggtatac atatgtttgg atgtggtgta gcagaactta taaatcatgc
    22621 agcatcattt aaagcgttag agatacctac tgatgaagca agtgagttga tatttggtca
    22681 tccatgtact tcagaagctt taatggaagc tttggcagat gtaaatggag aatgtttaca
    22741 cttacctaaa aaataaatct aaacataaat tattaattta agtctattat taatattaat
    22801 ttatatcata aaattttaaa acacttactt agccataccc cttattatgc aaataattta
    22861 tttgcataat aaggggtatt ttttatggaa aaatatagaa tatagaatcc ttgtagtttt
    22921 tgttgtacaa aaaagatagt aatataaaat aacattatca ttttagaatg atttcaattg
    22981 taattaggaa aagtgatttt ttt
//
LOCUS       DAMJWU010000051        22670 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMJWU010000051 DAMJWU010000000
VERSION     DAMJWU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.051"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(96..611)
                     /locus_tag="PC460_002204"
     CDS             complement(96..611)
                     /locus_tag="PC460_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF2601088.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(974..2044)
                     /gene="leuB"
                     /locus_tag="PC460_002205"
     CDS             complement(974..2044)
                     /gene="leuB"
                     /locus_tag="PC460_002205"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF2601089.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGSVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(2051..2542)
                     /gene="leuD"
                     /locus_tag="PC460_002206"
     CDS             complement(2051..2542)
                     /gene="leuD"
                     /locus_tag="PC460_002206"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF2601090.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2557..3834)
                     /gene="leuC"
                     /locus_tag="PC460_002207"
     CDS             complement(2557..3834)
                     /gene="leuC"
                     /locus_tag="PC460_002207"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF2601091.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3791..5533)
                     /gene="leuA"
                     /locus_tag="PC460_002208"
     CDS             complement(3791..5533)
                     /gene="leuA"
                     /locus_tag="PC460_002208"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF2601092.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5657..5903)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6051..6497)
                     /locus_tag="PC460_002209"
     CDS             complement(6051..6497)
                     /locus_tag="PC460_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601093.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTNESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6518..7237)
                     /locus_tag="PC460_002210"
     CDS             complement(6518..7237)
                     /locus_tag="PC460_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2601094.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7355..8629)
                     /locus_tag="PC460_002211"
     CDS             complement(7355..8629)
                     /locus_tag="PC460_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF2601095.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(8829..10196)
                     /locus_tag="PC460_002212"
     CDS             complement(8829..10196)
                     /locus_tag="PC460_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2601096.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(10446..10910)
                     /locus_tag="PC460_002213"
     CDS             complement(10446..10910)
                     /locus_tag="PC460_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2601097.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(10915..11544)
                     /locus_tag="PC460_002214"
     CDS             complement(10915..11544)
                     /locus_tag="PC460_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2601098.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRIIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(11578..12465)
                     /locus_tag="PC460_002215"
     CDS             complement(11578..12465)
                     /locus_tag="PC460_002215"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2601099.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(12494..13732)
                     /locus_tag="PC460_002216"
     CDS             complement(12494..13732)
                     /locus_tag="PC460_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601100.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(14116..15258)
                     /locus_tag="PC460_002217"
     CDS             complement(14116..15258)
                     /locus_tag="PC460_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2601101.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(15399..16265)
                     /locus_tag="PC460_002218"
     CDS             complement(15399..16265)
                     /locus_tag="PC460_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2601102.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(16599..18146)
                     /locus_tag="PC460_002219"
     CDS             complement(16599..18146)
                     /locus_tag="PC460_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2601103.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(18139..19275)
                     /locus_tag="PC460_002220"
     CDS             complement(18139..19275)
                     /locus_tag="PC460_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601104.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(19538..20635)
                     /gene="dinB"
                     /locus_tag="PC460_002221"
     CDS             complement(19538..20635)
                     /gene="dinB"
                     /locus_tag="PC460_002221"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF2601105.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVDVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(21025..22530)
                     /locus_tag="PC460_002222"
     CDS             complement(21025..22530)
                     /locus_tag="PC460_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF2601106.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
BASE COUNT         7461 a         4111 c         2345 g         8753 t
ORIGIN      
        1 ctaatttatt tttatcttgt atttttattg tatttttgaa ttttattttt tctaaatata
       61 aaatttatgt tttaaaatgt aaaattttta tttaatcaaa gttcaatata ttccactttt
      121 tctttcgatt cttcaaaaaa ccattcttca ttagaaaaat caccttctgc aatttgtcca
      181 ataggcattt tcatatactt tccagttgct gtcactgcta tatctccatt tggtagaatt
      241 atttctcctg taccttcaaa taattttctg ttttctttag ttattcttcc aacaactttt
      301 atagtagcat ctgttggtac tggtttctta tatttcaact ctaaagatac tgttactccc
      361 catatttggt catcatttat acttacagct cttccaatag tctcatccaa tattgcagca
      421 gacatacctc catgaactct cccaggataa ctttgatgcc aatcctttgt attacaaatt
      481 gatacaagct ctccattttc aagttcataa aaatctgctt taagtcctag ctcattttgt
      541 gttccacaaa ctaaacaaga tttactacta ttttgcttct tcaaaacttt atactttgcc
      601 aaaaaatcca tctcctaacc gacattagta gtcgcttaat attaaataat tctaaaatat
      661 attatataat attaataaaa tccctgcaaa cttctaataa taaaaatagg ctacttcaat
      721 atgataagtt taatttaaag tattattttc caaaaaataa ctttatcaaa atagaaactc
      781 aaagtagata taaacctctt tttcgttatt aaaatagtaa aaatatattt gacttaatat
      841 aaaaaagagt gtctcattaa ctaataaagt ttattttgag acacccctca taaaacaaat
      901 tatactacaa tagattttta ctaattctac aagttatata tttaaattat attattcaaa
      961 ttaaactata attctaaatt tcatttacta ttaattcgcc catttccctt gttcctacaa
     1021 catttcccac accattatag atatcaatag ttctataacc tttattgagt acactttgta
     1081 cagcatcttc aatacactta gcttcttctt ctaagttaaa actatgtctt aacatcatgc
     1141 ttacagatag aatagtcgca attggattta caatatcttt tccagctata tcaggtgcac
     1201 ttccatgtat tggttcatac ataccaaaac tatcttctct aagtgatgct gatggtaaca
     1261 tacctataga acccgtaatc atagatgctt catcagatat tatatctcca aatatattat
     1321 tagtcactat tacatcaaat tggctagggt cttttacaac ttgcatagca gcattatcaa
     1381 catacatatg tgacagttct acatcttcaa actctttagc taagtcttcc attatacttc
     1441 tccaaagtct tgaactctca agcacatttg ccttatctac acttataagt tttttatttc
     1501 tatttctggc tatttcaaaa gctctcttac caattcttct tatttcattt tcgttatata
     1561 tttctacatc ataagcattt tcgataccat caatgatttt tcttccacgt tctccaaaat
     1621 aaatgccccc tgttaattcc cttacaacaa caaaatcaat gcctttctca actatatccg
     1681 ttctaagtgg tgaagcttct tttatacttt catacattgt agctggtctt aaatttgcaa
     1741 ataaacccaa tgactctctt aattttaaaa gaccagactc tggtctttta tctcccttac
     1801 atttatccca ctttggtcct ccaacagaac caagcaatac agcttcgttt tttcgacaag
     1861 tttcaacagt tttattaggt agaggttctc cagtttcatc aatcgcgcat cctccagcta
     1921 atacatattc acaatcgaat ctatgattaa atttacaaca aattttattt agaaccttta
     1981 taccttcatc tataatttcc ggtccaacac catcaccttt tatgactgct atattacaat
     2041 tcatactcca ctacccccta tttgctttta ttgagttaat taagccttca tttaatataa
     2101 tattttgcat aaactcagga aatggttctg ctttatattc ttttcctttt gttatatttt
     2161 ttatcacacc tgtagaaaaa tctacttcta tattatcccc ttcatctatg ttattggcag
     2221 cttcttcaca ttcaagtata ggaagcccta tatttataga gtttctaaaa aatattctag
     2281 caaaagtaga agcaatcaca caggatactc cactttcttt tattacaatt ggtgcatgtt
     2341 ctcttgatga accacaacca aagtttttag ttgcaactat aatatctcct ttttttacct
     2401 tagaaataaa tttatcatca atatcctcca tacaatgcgt tgctagttct ttataatcag
     2461 ctatatttaa atatctagct ggtattatta catctgtatc tatattgtca ccgaatttaa
     2521 aaacacttcc attggcaatc ataaatttta cctcccttaa attatttctg atggttttga
     2581 tatttttcca gttatagctg atgctgctgc tacagctggg cttgcaagat acacctctga
     2641 atcaacatgc cccattcttc ctacgaaatt cctatttgtt gttgaaatgg atttttctcc
     2701 tgctgcaagt atacccatat gtccccctag acatggacca caagttggtg tagaaactgc
     2761 tgctcctgcc tctataaaag tggtaatata ccctttttct atagcttcaa gatatacttt
     2821 ttgagttcca ggaaatatca taactctaac tccatctgct acttttttgc ctttcattat
     2881 ctcagctact acttcaaggt ctgatattct tccatttgta caagagccta ttacaacttg
     2941 gtctatcttt atatccttat ctatctcatc tactgttcta gtattttcag gaagatgagg
     3001 aaaggctact gtttctttta gttcagctaa atctatttcg tatacttcat catagactgc
     3061 atcttcatct gcttcatata tatttacatc ttttgtcata gttgaacact tatgtgaatt
     3121 tatatattcc atagtcttat cgtctactgg aaaaattcca ttttttgctc ctgcttctat
     3181 tgccatatta caaatagtaa atctatcatc catagataag tattctactc catctccaca
     3241 aaattccatt gacttgtaaa gtgctccatc aacacctatc tcccctatta tatgaagtat
     3301 tatatcttta ccacttatcc atttatttgg tttattcttc aaaacaaatt ttattgctcc
     3361 aggaacctta aaccaacatt ttcctgtagc catacctgct cccatatcag ttgaacctac
     3421 accagtagaa aatgctccta atgctccata tgtgcaagta tgagagtcag cacctataat
     3481 tacatctcca gcagttacta tacctttttc aggaagaaga acatgctcta ttcccatatc
     3541 tcctacatca aaaaaattta caactccttt tgattttgcg aaacttcttg taaatttaca
     3601 ttgttctgca ctctttatat ctttattagg tgtaaagtgg tctaaaacca tcactacttt
     3661 agtcttatca taaacatctt caattcctaa tttttcaaat tctctaatgg ctactggtga
     3721 tgttatatca ttacctaaaa ctacatctaa atctgcttcg attaattgcc ctttcttaac
     3781 ttcatttaat ttagcatgtt tggctaatat tttctgcgtc atagtcatcc ccatttttta
     3841 tttcctcctc aatataactt ttatttattg cattcataag tgcattaata gctgctgtat
     3901 ttatgttttc cgatatactt acaccaaaat agacttcatt attgtgctct attcctacat
     3961 aagctacccc tcttgaatgt gacccttcct ctaatgcatg ttcccagtaa tatctaaatt
     4021 tataatcttt catgccacat tgctttagtg cattattaaa tgaatctaca ggtccatttc
     4081 ctataccaac aatcctttgt tcatttccca tataacttat tcttgcagtc atctcaatca
     4141 tattagtatc attagaatca tcattttcat agtttaattc atccattgat tttattttat
     4201 acttcttaac tttataaggg ctctcaactt caatatattc tttcttaaac aaattaaata
     4261 ttgcttcact actaagttct tccccaattc tatcagattc cattttgact acatttccaa
     4321 aatcagaatg catattttta ggtatcataa atccataatc tgtttccatt atataaacag
     4381 ctcctccttt tccagattgg ctatttatcc ttataatttc tttgtattct cttccaatat
     4441 catgagggtc tattggtaaa tatggaactt cccagtaatc attatctctg tttttcattg
     4501 ctatcatgcc ttttctaata gcatcttgat gtgaacctga aaaagcacaa tgtacaagat
     4561 tacctgcata tggatgtcta tcatgtacca taagttttgt acagtcttca tatataccta
     4621 ttatcttatc tatattgcta aaatctagct ctgggtctat tccttgtgta taaagattaa
     4681 gtcccacatt tactatatcc atattcccag ttctttcacc atttccaaat agtgttcctt
     4741 caagtctatc ggcaccagcc aataatccaa gttcacttgc agccgtacaa gttcctctat
     4801 cattgtgagt atgcaaactt agtataatag aatccctaca acttatattt ttacaaaacc
     4861 attctatttg gtctgcatat acgtttggtg ttgccatttc aactgttgat ggaagattta
     4921 ttattacttt tttttgagga gttggtttcc aaacatctat cactgcctca catatttcaa
     4981 gtgcataatc catctctgtt cctgtaaaac tctctggtga atattcaaaa gtaaattctg
     5041 tttctggata tttttttact tcttcattaa ataattttgc tccttcaact gctatatcta
     5101 ttatttcttg tttactcata ttaaaaacca catctctttg aagaacagat gtagagttat
     5161 ataaatgtat aattgctttt ttacatcccc ttattgattc aaatgttttt tctataagat
     5221 gagccctaga ttgtgtaagt acttgtatag ttacatcatc tggaatcata ttctcatcta
     5281 ttaattttct taaaaatgta tactctgtat ttgatgctga tggaaatcct acctctattt
     5341 ctttaaatcc tgtatcaata agcattttga acattctgac tttttcattt acactcatag
     5401 gtgttggcag tgactgattt ccgtctctta aatcgacact acaccaaatt ggagccttaa
     5461 atatttcatt atctggccat ttacgattta ctatttgcat tttatcatat tttttatact
     5521 ttccacactt catgttattt cccccttttt ctgttttttg tattcagtat acctcatatc
     5581 gacgcaatcc tgcacaattc ataaggataa atgtcaattt tatcattaga ataaaaaaaa
     5641 cctcatctct aataaaatta gagacgaggt tttgctcgcg gtaccactct atttagcttt
     5701 ctaaaaagct cccttagtaa tagccaatac aataattata atgactatat ttcctataac
     5761 gtggaattac tccgattatt ctaagattta aatcttagaa aacagctcct agacaagttc
     5821 agaactatca actactaaat cacaccatcc tttagctctc tgaaagtttc attgttctta
     5881 ctacttctat tcatcacttt tttaatcatt taaattttct aaatattttg atttaatagt
     5941 tattatatac acattaaaat gtattgtcaa cacaaaaaat aattatctca attttttatt
     6001 tttgataagc aattctataa aacatgctaa ctattgaaaa cagtatgata ttatgcctta
     6061 aactttttca tcttttcatc tatgatattc aataattttt tttcatcttc caaactcaat
     6121 gcccttaatc taattttata attagattcg ttagtgtata tatttaatat attttttact
     6181 acttctattt tttctattat cttataatta aacttttgac tttgtttaga ttttatactg
     6241 aatatacatt gattatttat taatattgaa tgattatcga gagtaatata tacttttccc
     6301 tcatatttat ttgaaaaatc acgaccttgt ttaaatagaa aaactgctgt tattatacat
     6361 aaaatatata atattgtcgt tgaatatata aaaattgtag ctaaaattat aagtgctaaa
     6421 accatatata tagtcattcc ttttttaaaa taaccattgt cataataatt catatagata
     6481 aacttatttt tactcaaatt ttttcactcc caagattcta aaaatttata tcaaaaaatt
     6541 ctataatttc atcttcatga taatttatga ctaattcatc tggaaaatct actgcttcta
     6601 aaactttttc tgcattttca aattctccta tagaataaca tatatgagag tcagtaccaa
     6661 gaattactct tgcaccatat gttttacaca atgtcaacat atgacttaca ttttcccaag
     6721 tacccgttct atgagagttt gaacttaatg atgaattatt tatttctaaa aggatatttt
     6781 tatctttagc tttttttact ataggttcat aatctaaagg atacctactg tcatctggat
     6841 gtccaattat ttttacctta ggtttatcca taacttttaa aatagcattt gtattttctt
     6901 cttttgtacc agaagctata caaggtctat gcaaacttgc gattatataa tccaaatgtt
     6961 gcaacatatc ttcttgaaca tctaaattac cattataatc cattatattt ccttctattc
     7021 ctctaagaat ctttacgcct tgcacttcac gtggaagcaa atgcaaatta taaaaataga
     7081 atggatgtgg tcctcctggc atattaggtc catggtctga tattcctaag tattttatat
     7141 tcttcttact cgcctcttcg acattttcct tgaaagtact atgtgcatgt ccacttatta
     7201 gagtgtgaca atgtacatcc ataatagact ttttcattaa atttcctcct aatattttac
     7261 ataaattcgt gaaaatagct aaagggctat ttcaaaacta aagctgtcat aatagacagt
     7321 gtaacgtttt aaaacagccc cattttcaat tattttacac ttctaaatct ttaaatttat
     7381 ctttaaataa ttctccaata cttcctaaag attcctctac ttctaggtat tctgttatat
     7441 cctcttctgg ctctctagtt gcttctttta ttgaaaccaa cattctctta gtctttggtt
     7501 taaaatcaag aatcttaact ttaacctcat ctccaatatt aacaacatta gtaactttag
     7561 ccactctagt atcactcaat tcagatattg gtaaatacgc atctacacct tctttaattt
     7621 gaacaaaagc ccctttttct attattctaa gaactttagc attgacaaca tcatcaattt
     7681 gtacttcttt tgctattaaa tcccaagggt cattatttat gtcttttaat gccaaggcaa
     7741 tttttttagt ctctttattt acatccaaca cataaacttc tacttcttgt ccctctgaaa
     7801 caacatcttc aacactttca actcttgtcc aagctaaatt atttatatgt gctaatcctt
     7861 caatcccacc aatatccaca aatgcaccat aaggcattat ctttgtcacc ttaccatctc
     7921 ttttttgtcc aacttcaaga gaattaaata agtcttcttt agctgctttg atacgagctt
     7981 tttcttcttc tctttcttgc ttaattcttg ctctttcagc ttctttttct gccttaattt
     8041 gagctcttct ttcttctctc tctttattta tacgctcttg taatacatct ctatgagatg
     8101 ctacaagtct attttttcta acatccaatt cttttatata aacttctaag ttaagaccaa
     8161 caaatttaga tgtatctgta ataaattttg tatcaagttg agaaattggt ataaaaaatg
     8221 tatatgtatt gtaattagca aatacacctt tatctatatt cttttcaaca tttacagtta
     8281 ttattctatg ttcatcataa gccttttgta actcggcaaa atcatttctt ttgtcaagtt
     8341 gtaatttaga taaagttatt gtaccatctt tttgagatac ttttgttatt acagcagtta
     8401 tttcatcacc tatgtggtat atatcttcta tatattgatt tttttctatg ttcaattcac
     8461 ttataggtat tattccatca aacccatagt ctaatcctaa ttgtacttca tcatttctca
     8521 ctgcagttat ttttccagtt gtcaattctc caactttcac tttgctgaat acttgttctt
     8581 gctggtcaag taattcttgc attgttaaat cattttccat tataaacatc ctttcctctt
     8641 attgattatc atatctgtaa atattatacc attaatattg caaatactta catttttctt
     8701 actataattt ttaaaataaa ttcaacttca tatatttata cattatagaa aatatcttat
     8761 taaattaaaa tacaaattct aatttttatc taataaaata ttttcataaa aaatttatat
     8821 tgtatatctt attcttttat tgctttaggt tttgatagaa aacatttata aaatataaag
     8881 aaagaaattg ctgttatagt agcagctgtt atatctgcaa ctggctctgc taaaattact
     8941 gcaaaaagtt tttcattaaa gaatgttgga agtataaata ttaaaggtat taaaagtaca
     9001 atctttctaa gtagagctaa aaccaaagat attgttgctt ttccaagtgc taaaaatgtc
     9061 tgttgacaag ctatttgaat cccaaacata aaagcaccag caaaatatat ttttatactc
     9121 caactagtca tactaagtaa ctgcggattc ttattaaata tactaacaaa aaatgcagga
     9181 aatatcatta ataaaagcca taaaatagta gaatatgtaa aacatgacaa caaacacaac
     9241 ttaaatgtct tttttactct atcaagttga cctgaaccat aattataact tattattggt
     9301 tgtgctcctt gtgtaacccc catattagga agcataagaa tttgcattat actgctcatt
     9361 atagccattg aacctactgc taaatctcct ccatacattt gtaatttact atttaaggct
     9421 ataagtacta aactttctgt agattgcatt ataaatggag agattcccaa accaagcact
     9481 ggtaagatga tacttgcttt tggtatcata tacttctttt ttattttaag tatacttttc
     9541 tttccaaaga gaaacataag tacccaaata gcagaaacgc cttgtgcaat tatagtagct
     9601 aatgcagctc cttttacacc catattaaag ccaaatataa aaatcgggtc aagtattatg
     9661 tttattaaag cacctatagc aactgtcatc attccagttg tagcaaaccc ttgggtattt
     9721 ataaaagtat tcatacccat tgaaatctga acaaacactg tacctattaa ataaatattt
     9781 aaatagtcca ttgaatatcc aattgtagat tcacttgctc caaacatcca aagtaaaggc
     9841 tcttttccaa tcttaaaacc tatagtcaaa agtattgcta aaatcaccag tacagaaaaa
     9901 ctgttactca ttattttctc tgcctcatca ttgttttgct cacccatttt tatagcagct
     9961 aatggacctc ctcccatacc gattaaataa ctacatgcag ttattataat tattattgga
    10021 aatgccactc caacaccagc catagcaact tctccatttt ccattctacc aataaaaatc
    10081 ctatcgacta tgttgtataa aacatttaca atttgtgcga taatagcagg tgtagcaagt
    10141 ttaaataata atttacccac actttcacta cctaagttag tgtttgaatt ttccatttta
    10201 aaacctcctc aatccaataa ttaaaatgta tcactaaaat tttattctat ataaaaattt
    10261 cattttatta gcagacaaat ttagagtact ttccctacaa gtaatactat actatatttc
    10321 ttttgccata gtaaacattt tagaactaac tgaagttaaa tactttatct cattttttat
    10381 ttattattaa aaatttttca cacatactca taaaaaaact atccaagtat attacttaga
    10441 tagttttata ttatatgtaa agaaagagct gaaactataa cccctgataa caaattgcca
    10501 agaaatactc ccataaaagc atcttttatt ctcattttta atatggaagc tatagcagat
    10561 gcagtccacg ttcctgtagt tggcagtggt atacctacaa atagtattat accaattatc
    10621 gaaacacttt ttaatttctt cattcttgag tttatttttc tatcaattac atcagcaatc
    10681 cctttaaata gtttcttccc tcttaaaaat tgtaatacat gtctgaatgt caatatcaag
    10741 ggaatcgata ccaatgtaga acctattaca ctaaatacat aaacccatat aggatttaag
    10801 cccattgcaa tacctatagg aatggctccc ctcaattctg ttacaggaac catagataaa
    10861 attaatatgt gtaaatatcc caaactattc cctcctattc taagtgacat taagttatga
    10921 atttatttgc tctataacct caccatcctt tttactaact aaaaatgcat ttttagtcaa
    10981 tgaaagcatt ggtaaatcac tcttagagtc tgaatatgca tatgaatttt cataatcaat
    11041 ctctatccca agtttaccta atacagcctt tattcttttt actttttcaa ttcccttaca
    11101 gttttctcca atgaatttat ttttatatct tgaattttca taaaaaaact ccgtgcctat
    11161 aacctcgtct gcaagaccat aatacttaaa atatttcata tatgcatatg gagatgcagt
    11221 tatcataatt ataacattat cttcttcttt atttttgtaa ataacctttt tcatagattc
    11281 gttaattttt ttcattatgc aatcatcaaa aaactttttt aaatcttctt cactaaaatc
    11341 tactattgga taaaagattg cctcttttgc cttcttcaat ggtattattc taattaaata
    11401 cataaataga ataaaaataa tgtatggtaa ctttataata gttttaaagg ttttattttt
    11461 taaagagtaa ataaaaaaat ctatcatgct atcagtattt ataacagtat aatctacatc
    11521 aagtaataat acactttttt tcataagtat ttccccatcc tatcactaaa aatatcacta
    11581 aaaaatcaat attattaaac atgtaattat ccataagatt acgtttattt ttaaagcatt
    11641 atcttttaag acaatatcag taggattatt ttcatttgta gtgttaatta tataattata
    11701 tctaagtaca ccataaacta caaatattgt tgtaaatatc atatgtggct tttctggatt
    11761 acttgtagaa tacaatgcat aacatacaat tgtagaactt aatataacta tcatcatttg
    11821 atttagattt tctatgtcat aatcttccaa tatttttcta tgttcaactg catcatctct
    11881 tagagtttct atttcttttt tacgttttcc aagagctaag tacagagata agaaaaatgt
    11941 acataaaatt atccaatttg ataaactaac acttatggct acacatcctg cataaaccct
    12001 aagtataaaa ccaagagata ttgaaaacac atctaatagt attatattct ttagtttaag
    12061 agaatataat atattgttta tcaaatagag actgagaatt atagaaagat taaaactaac
    12121 aactgttgat aaacagactg aaattaaagc taatactaca aatagtatat tagcactttt
    12181 tttgcttatt cttccacttg ccactggtct attacatttt ttaggatgtt ttttatcttt
    12241 atccatgtct atagcatcat tcagtgcata tactgatgat gacattagac aaaaacacac
    12301 aaacgcaata aacgtattta aaaataaatt tatatttagt atgttgtttg aaaaaattat
    12361 agctgaaaat acaaagaaat tctttatata ttgctttatt ctcattaact ttatataatc
    12421 tgaaatttta gtaagaagcc ttatacttgt attggtctgt ttcataaact tactaccctt
    12481 tcaatattat tttttaaaca ggatatgatt ttatccctat gtttaaaatt tctataaatc
    12541 cattgacatt actgattact atagtaacca ataacagcga tagaaatata ttatacatat
    12601 tcttttcatt ttttctaagc gctttcgtaa agtaagccaa taaaattata actgcaaaac
    12661 taaaatttat agccataata cttgaattga ctagattata tcctactata ccatgtaaag
    12721 ttagattgaa tataattatt cccatacata gcttaggaac tcttttttct gaattttcaa
    12781 taactgataa aacacaaaaa cataaagcta ataatgtaag aattactggt attgtagcaa
    12841 cttctccatt ttgcaaaaat aacccttttt taatacttgc tccaggaaaa taaaaaaccg
    12901 aatacaataa tctctctata atcattgcaa tcttttccaa aattggttct ccacttataa
    12961 atttcgtatc atactgtatt acatttccaa ggttcataaa actctcataa gactttgttg
    13021 aaactacaca aatatatgcc attaggataa atgtaattaa atgttttaac atttttccta
    13081 tttttaattt aaactcaaat atcatattaa aaaaatacaa atatatatta ctaatagtta
    13141 ttccaaatag aggaatagct gctaattcta ctaaataaga attttttcct ttcatcttat
    13201 ttgcaataac tatgaaaaat attaatgaaa attggctata tataaatctc tctacattta
    13261 aagtcataaa taactgtgga aaagaaaata tcattatcat agtaagtaaa attaaagtta
    13321 atctctgcat ttttatcttt ttaatatgat taaagactaa agttattgac atagcactag
    13381 ttattatttg tagacaaatt agtccaaacc cataggcact attaaaatct gttgtcccag
    13441 gatttgctat tgaagtacaa aataggtata ttggatatgc aaaaattgat ataattgctg
    13501 aaaacaatat gtgcttacta ttgtcttgac ttatagcaaa tacatttaaa ttgaataaaa
    13561 ctcctgtatc tgtatctaac aatacatcat aagtattgtt taaatcataa aatatagtag
    13621 tcttagaaaa aaatatctta gttattatta agtaagttat tacaactaat ataaacaaaa
    13681 acatctcgtt tttatatttt aatattttac tcatcaatat atcaccatcc attcattata
    13741 taggatttgc tgacacctac aatttattcc ttatatttaa tagccatctt cataatgttc
    13801 ttacttgttt tattgtaatt gaataactct ttatttacaa ccacaatatt tactttattg
    13861 atgttataat cagtcaagta ttgtttatat aacatagatt agtgttgtat aaaatgagaa
    13921 tttgtaagca atattaatgt aaaatatttg aaaaacttta tataaaaata tacaatgttg
    13981 cttatggaat taaactataa caaattaaag cataatttaa ggagacaaaa ttataatctg
    14041 tctccttaaa tattaaaact atattttaaa acattactaa atactataca aatttcaaat
    14101 atagtataaa attaattaga ctttaacact attagctcca tcttcttccc attttttaat
    14161 ttcttttaat ctatctatta taggtaaatg ctgagtacat tcaacttcac attttccaca
    14221 agatatacat tctttagctt ttgccttact attcattgtg taattccaat gtcctaagcc
    14281 ataaactaac tttgtcattt cttcatcact ctttttaaac atctgctttt cattataaaa
    14341 aagcatatag gctggggtat taataccttt tggacatact ttacaataac cacaacctgt
    14401 acatatttcg ttcatattct tatttaatct agtctcaata tcatgtatat cctcatctga
    14461 ataaattcta ttttcgtctg caattttaca tgcatcatct atatcttgtt ttttattaaa
    14521 ccctatcaaa gatatactta tttgtgaaat tcctatgttg aatcttaaag ctgattctat
    14581 agtactttcg ccatcttttg atagaaaaga aagttcttta ttatactttg gaatagttcc
    14641 accacttaat gggttcattg ccacaacacc atatccatta tcaacagcat atttggcacc
    14701 ttttagccta tatggaaagt ttaatatatt tattcccata gttacacctt caaacttatt
    14761 ttcacttaga atatctataa cctcatctcc tggttgatga gatgaaaata ctatatggtc
    14821 tatcaatcct tcttctttac atttcaataa tccttcatat tgacctccag gtctcattgc
    14881 tagttcgtaa tgttccatct ttctaatatt ccaaacatga taaaaattta ttttagatac
    14941 gcctaatcgt tctattgatt tttttacagc ctctatagct ttttctgatg tatcacatgc
    15001 ttttggcttt tctttagttg atacataaaa atcggtctta ccttccttta tcatttgtct
    15061 aaaggctagt ccaaatatat cttcacttct gtcatcacaa taccctggtg ctgtatcaaa
    15121 gtaattaata cctttttcat acgcatattt gactaaatca gcattttctt gattactttt
    15181 ttctaaatca aaccttaatc ctccatagcc aactactgaa accattttcc ctgtctttcc
    15241 atattcaacg tatctcattg taaattcctc cttagatata ttaagactta atttctttat
    15301 agaaaatatt ataccataaa taatttttat aaattattta tggtataata ttttttttat
    15361 aaattatatt tttttataac ttgtatttta taactaagtt aaaaactata aaaaggttca
    15421 atatggacta ttacttgagc attttcatta atttcttctt gtatttgttt ctcaatttta
    15481 tgtgttaaat catgagattg ctctacgctt ataaatgggt ctaccattac atgcatgtca
    15541 atataaatag aacttttgct acctctactt ctgatattgt gaacatccct tacctcatta
    15601 aattcaaaca caatttcttt tatataatcc tcatctacag ccactctatc gaccaaaatt
    15661 ccaatagatg gcctaaatat tccataagcc gaataaatta tgaatgcaga aataataata
    15721 ggaactatag attctattat tactggaaat cctaacttaa caccaactaa agtaaccaat
    15781 acacctagag acacaaatat atcacttctt gtatgcaatg aatccgaaat caaaacataa
    15841 ctatttagtt tagtcccaat tctatattca tacatgcata caaaaatatt tataaaaagt
    15901 gtaataatta aagcaattaa actttctata gttatagcag gtacaactgg attagctatt
    15961 cttaacactg cactaagaat tatcttaatt gctataacaa acaacatacc acttataaat
    16021 aaactggtta taatttcaaa ctttttatgt ccataaggat gatttttatc ttttggttta
    16081 gatgcaaaga aaatacctat aagccccact atattagatg ctccatctga caaagaatga
    16141 aatccgtctg ctgtcatact atagcttttt atttgattac caataatgat ttttaataat
    16201 gctactgcaa aattagcaaa taaaattatc cacaatactt gcttcacttt cttatagtta
    16261 tccataacaa attccccctc ccttcgattc aataaagaaa gctatctatc aatatcaatc
    16321 aaccagacaa taaaatcgta tataagcaca tttactatat ttatattcaa aattggttgt
    16381 acttgttcat tctatttgaa acttaattat aaataaaaat cgtaggtatg gttttggtat
    16441 acataaaaat aagagactgt ctcaaaataa agataatctt cttttttatt gttgaaatga
    16501 taaaatatat ttgatttcat ataaaaaaga gtgcctcatg aactaaaaag ttcattttga
    16561 gacactccct taaatttttg atataaacta ttaatttttt atactaagcc ttctttaact
    16621 aagaatgaat gagcaacttc ttctggtgat ttaccaagtt catccacttg atagttaagt
    16681 tctctcatag tttcttcatt tatttttcct gaaagcatat tcataacttt ttctaactca
    16741 ggatatttct tcaaagtatc ttctcttact aatggtgctg cataatatgg tacaaagaaa
    16801 tttttatcgt cctctaatat cttaagatta aactttttaa taagaccatc agttaaaaat
    16861 gcatctataa cctgagattc attattatct aaagctgtat atcttaaact tccattcatt
    16921 gcttttacat ctttgaattt taaaccataa tctctagaaa gccctacaag accatcttct
    16981 ctattttcaa actccaaagt tggtgagaat gtaaattctt taccatattt agtcaaatca
    17041 gaaatagtat ttattccata cttttcagca gtttcaggca tcatagctaa tgtgtaagta
    17101 ttattaaatc caagagggtc aagtaaagtt aactgatttt ctttttccat agtatctttt
    17161 acaacattat atgcttcatc agcatcttta atcggagcat gtttcattac atttaccaaa
    17221 agtgtacctg tatagtcaac atacatgtct aactcaccag atttaattgc attaaacgca
    17281 acagatgaac cacctagatt taaattcttc ttaactttaa gattcgtatt tttttctact
    17341 aattcagcat aaacattgcc aagtataagt tgttctgtat aatctttaga accaatagtt
    17401 attgtatctt ttttacttga aaaactagaa ctaaaaacaa cgaacaacat tgaaacaaca
    17461 agcactacag atattacttt aagtgctata taattcttct taggtgcttt tggatttaat
    17521 ccttttggag taactgcaac ttcaatttta ctaaataaat aatcaactaa cagtgcaatt
    17581 atacatgatg ggattgcccc tgctaaaatc atattattat ttacagtttg aacccctgaa
    17641 aatactaaat atcccaaacc accagcacct ataaatgctg caagagtcat caaaccaaca
    17701 gctgtaactg cagatattct aactccaccc attattatag gaagcgctaa aggaaattgt
    17761 attttaaata atgtctgatt tcttgtaaga ccaatacctt tagctgattc taatactact
    17821 gggtcaatac ttgttatacc tatatacgta ttttttacaa ttggtagtaa tgaataaacc
    17881 actaccataa atatggctgg tttacttcct ataccaagta taggtacaag aagacccaaa
    17941 agtgccatag acggaactgc ttggactaag tttacaaatc caagtacaaa ctttctaaga
    18001 gatgagattc ttgaaataat tattccaaga ggaacaccaa ctattatagc tattaaaata
    18061 gatgtaacag ttagactcat atgttgtatt aaaagctcta taatcttatc tttttgtaat
    18121 agtataaagt taaaaaaatt agccaagaac actcacctcc atttctaaga attgttcact
    18181 taaaacagaa agtaaactac ttctagtaat aagccctacc agtttatttt catcagaaat
    18241 aaccggaata tatccaaccg aattacgatt cattacattt agtatttcta ctaaattatc
    18301 accttcattt acatgcaaag gctcacttga cataatatca gcaagtaata ttgacttatc
    18361 atcaatatct ttcatatctt ttacagtaac aatacctttt aatgtttttg ctctatctat
    18421 aattagtaag ctatcaacct tactcgttct cataatttct attccttgag ttacagttct
    18481 agtagaatta acagcaatag gattttttat cataatatct cttgctttta tatattctgg
    18541 attatcccaa actctatctt ctccgataaa atcttttaca aagtcatttg caggctttct
    18601 aagtatattt tcgggagtat catattgtgc tattcttcca tccttcataa tacaaatttt
    18661 atcagctatc tttaaagcct catccatatc atgagttaca aatattatag tttttttcaa
    18721 ttcatcttga agagaaaata attgttcttg taaagatgtt ctcgttattg ggtctaaagc
    18781 actaaaaggc tcatccatca atattatttc agcatcagta gcaatggctc ttgcaacacc
    18841 gattctttgt tgctgtccac cactaagttc tgaaggatac ttattcaaaa attcatcact
    18901 atcaagtcct accatctcaa gtagttgcaa cgttcttttt tcaatttctt caacggattt
    18961 ttcctttttt aggcgtggaa ttagctctat attttctttt attgtaagat gagggaatag
    19021 gcctgtgttc tgtataacat atcctatttt tcttctaagc tctatttcat tttcctgaga
    19081 aattggtttg ccattaatat atatctcacc agatgtaggt tttattaatt tgtttataag
    19141 cttcaaagta gtagtctttc cacaaccact tgaaccaatc aaaactacca atgtacctgt
    19201 ttctacaact agagaaatat catctaatat aacattattt cctatttttt ttgttacatt
    19261 tcttatctct atcataattt atcatcccct ttttcgaaca agcaaccttc ttcaatatta
    19321 taagttgttt ttataattat atcatactat tttatttatg taaacaaagt acgattttta
    19381 ggtcactatt tgtctgaata ttgctacaaa tataaagtat ttatatatag tatataatac
    19441 ataatgtcaa aaaacaaaaa atataaaaat attcataaaa taacactagc ctcaattata
    19501 tagtattaaa gctagtgtta tttcatttaa acaaaactca catttggata ctatccatgt
    19561 attcaaataa tgtaatatct ttataagttt cttttttatc acttaggttt gatattgtaa
    19621 ctcccaatag cctaatttgt tttccattaa catcaacatt tctcaacaaa ttatctacac
    19681 cacttcttat atcattatat tcgtcaatat agtgttctaa actaaggctt ctagttatct
    19741 ttgtaaagtc ctcatattta atttttaatg ttaatgtttt tccaaacttc tcagagtttt
    19801 ttaatctatg acatacttct tcacaaagtt catcaagaat gttgcgtgtt tcttcttcat
    19861 caatatccaa attatgactt aatgtagtct cagccccaac agattttcta accctatttg
    19921 gttccactgg tctatcgtct attcctctag caaatttata taattcataa cctctttttt
    19981 tgaatatatt ttcaagttca aataaattta aacaccttaa atcatatcca gtttttatac
    20041 ctaaattttt aagagtattt gaagtaactt ttccaacacc aaaaaattta ttcacaggta
    20101 atttatctaa aaagtcctgg gcattttctt cggttattac agttaaccca tttggctttc
    20161 ttaagtctga tgcaatctta gcaagaaact tattgtatga cacaccagct gaagaagtaa
    20221 gaccaacttc tctaaatata tcttttttta tttgctttgc tatctcaatt gatgaatcaa
    20281 tatttttttt attttttgtt acatctaagt aagcttcatc taaagataat ggttcaacta
    20341 aatccgtgta tctataaaat atatctctta tttgctctga cacactctta taaacatcaa
    20401 atcgaggtct tacaaaaact gcatatggac acctattgta agctacagct gatggcatag
    20461 ctgaatggat accaaatttt ctagcttcat aagaacacgt tgcaaccaca cctcggcttt
    20521 gtggatttcc accgacaata acaggtcttc cttttaattt tttattatct ctttgttcta
    20581 tagatgcata aaaagcgtcc atatctatat gaattatttt tctataatgt ttcattatta
    20641 tcacctttta aagtataaat agattaattt tatttatatt actttttaat tataccaaac
    20701 ttatgtttct ttttggtatt ttttcattat tttctttata tattcatatt ttccataaat
    20761 aaaaccatct ataatccatt aacttcaata taatttataa aaaatcctta aaaacacttg
    20821 caaaaactat ataaaagctt tacaataggt attccaagga actaaattta aaattcctta
    20881 tggataacag tatatatatt taatttatgg gatgttttaa aaacaaaaga ctgtcttatg
    20941 caagacagcc tactgatttt attataattg taatggactg ctcaaactat tactttaaaa
    21001 cagcccatat ttttttatat aattttaact atagtaaaga ttttgtgaag gatattctgg
    21061 gtcacaagtt acatctatac caagctttct aaatgtttgt tcatcatctc tatttaatat
    21121 agtagtagaa tgagcttgac acccttttaa taatgctaac ttttccatag caaattgtgc
    21181 agttggatta gttacagcac aaatactaag tgcagttaac acctcttggc aactaagtga
    21241 aacatttcta ctagctaaag tctttgtttt taagtttatg ataggttcta atattacagg
    21301 tgatattaag tgcatatcat cagatatatt ggctaaatgt ttaatggcat ttaatactac
    21361 tgctgcagtt ccattcatta aatcagaacc cttaccagtt aatattgtac catcttctaa
    21421 ttctaatgca actacaggac aagtatcatt cttgtcagca ctttctttca atttattact
    21481 gtagtttcta gcaggtaata caacatttct atcttctggc tttaagttaa gctcttccat
    21541 aatcattttc attctatcat aagcaccttt atcaacttgt ccctttttat actcacaggc
    21601 agttttaaag tatcttctta ttatttcttc tatagaagct tttcttatta cttcatcatc
    21661 tattattcca aagccaactc tattaacacc catatcagtt ggagattgga aaattgattc
    21721 ttttccagtt atcttttcta ttattttttt aagcactgga aataattcca aatctctatt
    21781 gtagtttact gacatttcac catacgtttc aaggtggaat gaatctatca tattcacatc
    21841 ttttaaatca actgtagctg cttcataagc tatattcaaa ggatgtttta caggtacatt
    21901 ccaaactggg aatgtctcaa acttagaata tccaactaca ttacctcttt tattttcatg
    21961 atacaactga cttagacaag taccaagttt tccactatta ggtcctggtg ctgtgactac
    22021 tactattggc tttgtagttt ctatatattc atttgctcca tatccctcat cactaactat
    22081 agtctcaaca tctgacggat atccctttgt tggataatgc ttataaactt taattcctct
    22141 acgttctaat ttatttataa atacagttgt agcaggttgt ccttcaaatc tagtaattac
    22201 tacactattt acatctagtt cataacctct taaatcatct attaatctta aaacttccat
    22261 atcataagtg ataccaaagt ctcctcttat tttgtttctt tctatatctc ctgcatagac
    22321 acaaattaca acttcaactt tttctttcat atgttgtaat acttttatct ttgcattttc
    22381 atcaaatcct ggaagtactc tttttgcatg aaggtctccc atcaattttc ctccaaactc
    22441 taaataaagt ttgtcaaaat tatttactct ttctaagata tatttagact gctcttctaa
    22501 atattttttg tggtcaaatc ctactttcat cttaacacct cactaaatta aatttcttat
    22561 tgataacagt atatttatat atattttttt agtaaaacat cttataaaaa aatgttaata
    22621 ctttttcact caaagtaata ttatacaatt aagattaatt tttttcaaga
//
LOCUS       DAMJWU010000052        22524 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMJWU010000052 DAMJWU010000000
VERSION     DAMJWU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.052"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            227..937
                     /gene="rgaR"
                     /locus_tag="PC460_002223"
     CDS             227..937
                     /gene="rgaR"
                     /locus_tag="PC460_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF2601107.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNTITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            1983..3014
                     /locus_tag="PC460_002224"
     CDS             1983..3014
                     /locus_tag="PC460_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2601108.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            3147..4445
                     /locus_tag="PC460_002225"
     CDS             3147..4445
                     /locus_tag="PC460_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF2601109.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(4559..4804)
                     /locus_tag="PC460_002226"
     CDS             complement(4559..4804)
                     /locus_tag="PC460_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2601110.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            4952..5818
                     /locus_tag="PC460_002227"
     CDS             4952..5818
                     /locus_tag="PC460_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF2601111.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(5822..6358)
                     /locus_tag="PC460_002228"
     CDS             complement(5822..6358)
                     /locus_tag="PC460_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF2601112.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(6549..6752)
                     /locus_tag="PC460_002229"
     CDS             complement(6549..6752)
                     /locus_tag="PC460_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2601113.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            7043..7807
                     /locus_tag="PC460_002230"
     CDS             7043..7807
                     /locus_tag="PC460_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2601114.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            8196..9509
                     /gene="prdC"
                     /locus_tag="PC460_002231"
     CDS             8196..9509
                     /gene="prdC"
                     /locus_tag="PC460_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF2601115.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      9913..9998
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            10580..12829
                     /locus_tag="PC460_002232"
     CDS             10580..12829
                     /locus_tag="PC460_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDF2601116.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTSDVEKPSDGQVPDGEKPSDSQVPDVEKPSDGQVPDGEKPSDSQVPDGEKPS
                     DGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQ
                     TPDVEKPSDGQMPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINK
                     IVYNFWEDEEDVKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYH
                     DLRFDDITKTLTFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSP
                     YLPAEDFNGQENQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWI
                     ILGQMDEQERLKQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAG
                     REELKPNNTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDG
                     DGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKD
                     HSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPT
                     DDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPP
                     VPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSYVFISVIAICGL
                     AYQCKIKRN"
     gene            13236..14996
                     /gene="prdR"
                     /locus_tag="PC460_002233"
     CDS             13236..14996
                     /gene="prdR"
                     /locus_tag="PC460_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF2601117.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            15425..17305
                     /gene="prdA"
                     /locus_tag="PC460_002234"
     CDS             15425..17305
                     /gene="prdA"
                     /locus_tag="PC460_002234"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF2601118.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            17386..17670
                     /locus_tag="PC460_002235"
     CDS             17386..17670
                     /locus_tag="PC460_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601119.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            17708..18433
                     /gene="prdB"
                     /locus_tag="PC460_002236"
     CDS             17708..18433
                     /gene="prdB"
                     /locus_tag="PC460_002236"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:18158..18160,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF2601120.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            18513..19271
                     /gene="prdD"
                     /locus_tag="PC460_002237"
     CDS             18513..19271
                     /gene="prdD"
                     /locus_tag="PC460_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF2601121.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            19286..19753
                     /locus_tag="PC460_002238"
     CDS             19286..19753
                     /locus_tag="PC460_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2601122.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            19771..20253
                     /locus_tag="PC460_002239"
                     /pseudo
     CDS             19771..20253
                     /locus_tag="PC460_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
     gene            20273..21280
                     /locus_tag="PC460_002240"
     CDS             20273..21280
                     /locus_tag="PC460_002240"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF2601123.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            21400..22281
                     /locus_tag="PC460_002241"
     CDS             21400..22281
                     /locus_tag="PC460_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2601124.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         8982 a         2554 c         4098 g         6890 t
ORIGIN      
        1 taataaatac atattatatt tattttacta tattttttta cataatatat ctagaaaagt
       61 ataggaattt atcgtaattt aagtttaaat attaaagaga atttctataa gtagtttaat
      121 cgacaattta aatttataaa gaaatttaaa tttgaaagct agtgagtaca gtttggaaat
      181 tgaaataaag taaactaaat gatattttag atttggaggt gatattatgt tcagggtagt
      241 tatatgtgat gacgaaaaaa tacaaagaag catactaaaa gagtttacag aaaaactttt
      301 gtctgaaaga tgtctaaatt atcaaatact ggaattttcc tgtggagaag atttaatatc
      361 caaataccca gaaaaaattg atattatatt tttagatatc caaatgaaag atatcaatgg
      421 aattgaggtg gctaaaaaaa taagaaagtt tgatgaaaaa gtagaaatta tatttacaac
      481 ggctttttca gagtatgccc cccaaggtta tgaagttcgt gcttatagat atttagtaaa
      541 gcctatagag tatagtaact ttgcaatggg tgtaaatctt tgtattgata accttcaaag
      601 aaagaaggag cgttatatag ttttaaatag taaaaaagga tttaatagaa tactgataga
      661 ttctatttta tatgttgaaa ctgtaaagag agatttagtt gtacatacaa taggtagaga
      721 ttataaagct aatatgagca tgaaacaagc agaaaaatta ctgtgtgaaa atggcttttt
      781 tagatgtcat acaagtttta ttgttaattt aagaagaata gaagatctta gggataatac
      841 gattactatt aatgaacaat ttatacctat aagtaagtat agacttaaag atttaaaagt
      901 agcattaaca agcttgttat gtgatataat gtattaattt taaaaaggta attgaaaata
      961 tcgtaaaaaa gatttaatgt catcacaggg ctattgattg aaactgtctt aatatataaa
     1021 atgttattta tattaagaca gttttattat gcaaaatagc agtttataga cttttaatat
     1081 ggtttaatat gtatatgtag agtttccatt ataatagcat gattattaat tagtagcttg
     1141 attatcaata tactattatt gatttttata tgtattatat gtatttttag aatatgatta
     1201 cctttttaaa ataatgacta tttataaaac tctatagata tttgtttcat ttagctatgc
     1261 atactttaat taattatatt aatatttaat ggagtacaat ataaatatta aagcgacatc
     1321 ttatagttta ttgaacctat agtaatgaat taataaatag tttaattcta tgaaaagatt
     1381 tccttttatg caatttattt attgaaatat gatataatat ataaaggtgt aagtattagt
     1441 tgttaaaata atatttatat tagtttttat gcatacttgg tataagtttt tcatatattg
     1501 attaaaattg atgttgattt attattttta gtattttttc accttttgat tcatactttg
     1561 tagaatggtg tattttctaa aactgattat aagacaagtt cattcaaatt agcattttta
     1621 tatcatataa ataattgttc cccctaaata agatttagat gtatagtatt aaaatcaaaa
     1681 agtataaaat aaaattgatg cttaatcata gataaatatt aataaaatat acaaggggat
     1741 tgtctaataa aaagataatc tcctttttta caaataattc taaattgtga tataaatcta
     1801 aaaattttat cttaacattt aaccatttaa tgataataat tgatataata aatataaatt
     1861 aaatatatgg tgtacttatt ttatgttttt agcaagaaat atagcattta aaagataaat
     1921 tgtgcaactt aaatcaaagt ttaagatgaa ctattaacat agatatatta aaaacttttg
     1981 taatggagaa tatatgtgat atgaataata aagttaaggt tgcagtatta gatacaggaa
     2041 tagacaaaga acatgattac ttaaaagata atttagttgg aggaattgct tttgaatgta
     2101 tgcatgatta tatctttata tcagataaat ttgatgatga agatggtcat ggaactgctt
     2161 gtgcatcaat aataaagaaa gagtatgaag atgtagagtt atttgtaata aaaattttga
     2221 gaaaagatgg tattacaaat ataaaagttc ttgaagaagc attaagatac cttttagaca
     2281 ctaacataag attaattaat ttaagtttat cagtcatagg agtagaaagt gttaagggct
     2341 tatttgaagt ttgttatgaa ttgtttagaa aaggaaaaat aatagtatgt tcactcgcta
     2401 atgattttga cttaagttat ccagctatgt ttaacaatgt tataggtgta agaggattta
     2461 ctttagatgg agatgattca ttttggtaca acaaaaaata tgatgttcaa tgtgttatgg
     2521 atagcaattc atatataagt tgtgatataa ataattccta tagattacca ccaaaatgta
     2581 atagttattt atcagcaaag cttactggta aaatagctaa gatattatca gaaaaaccag
     2641 gcataacaat atctgattta aatgataagc ttgaatcatt agcaactaga aatcattggg
     2701 atagttgtga ttttgatgag tgtagtagaa ttgtaaactt taaattggat ttgtatgaca
     2761 aagaaaatgc tctttttatg aaagttgctg acgttgttag ggattgctta gatattgaag
     2821 agaataatga aaaactcttt aagggtagcc tttttaataa ggagataggc ttggtttatg
     2881 ataattgttt taatctgttg aaaaagctag agcatagatt tgacataaag tttaattata
     2941 tggacatctc aaaatatgac ttagtttcaa tatatacact gactgaatta gtggaaagat
     3001 ataaaaactt ataaataaag atttgtagct aaatttaatg tagaaggtat taaattatat
     3061 gtattctttg tcggtagatt aaaagtaaaa aatatactat aattagatag cataagagaa
     3121 aatgccaata cagggggagt tagataatga aatatgaaga agcattagag tacatatcac
     3181 aaactaataa attcggtata agattaggat tagaaaatat cggaaagtta ttagaattat
     3241 taggaaatcc acaagaaact ttgaatataa tacatgtagc tggaactaat ggaaaaggtt
     3301 ctgtgtgttc ttttgtttct aatatactaa gagagtgtgg atataaagtt ggtttatata
     3361 catctccata tttagaaact tttactgagc gtataagagt aaatggacaa aatataccac
     3421 aggatgatgt tgctagaata attgagttaa taaaagaaaa aatagagatt atggtaaaag
     3481 aaggctatgc atatccaaca gaatttgaag tggttactgc aatggcattt tattactatt
     3541 cagaacaaaa ggtagacttt gtggcattag aagttggttt aggtggcaga tatgatgcta
     3601 caaatataat aacaaaatca cttgtaagtg tgatagtatc tataagtttg gaccatacag
     3661 gtatacttgg agatacaata gaaaaaatag cgtacgaaaa ggctggaata ataaaggaaa
     3721 atggagtagt actagtatat gaccagactg atgaagcaaa ggatgttata aagtcagttt
     3781 gtaaagagaa aaaagctaaa tatatagaag ttgattttga tgatataaac ataaaaaaat
     3841 cagatataaa ttctcaaata tacgattgta ctgtaatgaa agaaacttat agagaccttg
     3901 aaataaaatt gataggcgaa catcaaataa acaattctat cttggcaatt agtgtaataa
     3961 aatatctaaa agacataaat aaattagcaa atataaatga agaatctata agaaaaggac
     4021 ttataaatac taaatggcca ggaagaatag aaaagataaa agaaaatcca atttttataa
     4081 tagatggtgc acataatgaa gatggagcaa aatctttggc aaaagcactt gataagaatt
     4141 ttaaaggtag aaaattaacc ttattaatag gtatgttaga agataaagat attgatagtg
     4201 ttttagagat attgttgcca cattttaata aggtaataac aacaacacca agtaacccaa
     4261 gagccataaa ttcagatata ttaagagaaa aagtattaaa atatgtagat gatgtaacat
     4321 caaagcatga gatagaggat gcagtaaatt atacattaga aacatcaagt gaggatgata
     4381 ttataataag tgcaggttca ttatatatga ttggtacagt gagaacttta gtaaagaaat
     4441 tataatttta taaaataagt atacaaataa aaatataaaa aagagttgtc taaaaaatgg
     4501 acaactcttt ttatataaaa taagttttta ataattttac tgatgtttat ataagtaatt
     4561 atatagattg acgtatagct ttagataatt ggtctgggca agaagtacct tttccaccac
     4621 agtcaatacc atttaattta tcagcaactt caagagcatt ttggccaatt attaagcttc
     4681 taagtcctga taaattacca ttacatcctc ctataaattc agcatcaaca atcacattat
     4741 tttcatcaac ttcaaaaatc atttctctac aacatacacc acttggatta aatgttactt
     4801 tcattaaaaa gacctccatt aaatattatt tcttttttta acattaataa taataccaaa
     4861 tatatgcgta ataatgcaac tataaagcat aatacaagct aatgaaataa tatattattg
     4921 tagtatatgt attgtagaga ggaggggtta tatgaaaatc tatatttcta actacttatc
     4981 aaagagtatt agcatagttg attattctac acttgaacta gaaaaagaaa tagtattaga
     5041 agataatata tatccacacc atttttgcat agaaaaagaa aaaaatttaa tatacatacc
     5101 tagttcaagt aatgggatac tctatgtctt agatttaagt aatgataaaa taattgatac
     5161 tgtctctata ggaggaagtc ttagtcaagt tgtacttagc gataatgaat tgtttgtggc
     5221 aaatgaagat tctaatagta tatatgtact agataaaaac acattaaatc caataggaat
     5281 tattggagtt gataatatgc cacatggatt tgactttgat agagaaagca aaaagttata
     5341 tgtaccgtgt ataaactcga tagtatgtat agatacaatt aataaaaata tacaaaagaa
     5401 aataaatatg gactttagag cttggcatat agaacttgat aaacaaaaga aagaaatgta
     5461 tatatcaaca cttgatggaa agcttataat aatagatgaa gtaagcatgg atataaaaaa
     5521 ggtattatat gaatttttat taccagtaga aataagattt aattatagtg gcaaaaaagt
     5581 ttatgttgct gatttgggtt ataataatgt aagaatttta gattatacaa cgagtagata
     5641 cattggaaat attgaaataa atgggattcc ccaaggatta gaaatatcta aagatgaaaa
     5701 gttactattt gtatcagata cacaagaaaa ttcagtaaaa gtttatgaaa ctgcaaataa
     5761 caaattaata aaagaaatca aggtaggaaa agagcctaca actataattt gtttgtaggc
     5821 tttaatgtat gtctcttata agtaaatcga taacttcggc atacatataa gatgcattat
     5881 ttttccagtt attgcaaata agttttgctt gtttctctga tgaaacattt agatttaaat
     5941 ctattaagtt agactgattt tctatgactc taaggttgac aataaattca ttatcacttt
     6001 gttttacaaa actagacttt acttgagtat ctgctagtag attttctttg ttagaatcta
     6061 tatacttgtc tattttttca gtaacactag taggtattcg attaaagaag tattctagac
     6121 tttctacgcc tctttgagta agagcataat attctctgtc agagtcttta tatgtagtca
     6181 aaaacttaga ttccataagc tgagataaaa gctgttgtaa tgaaaaataa ttcatcatct
     6241 cagtctctaa tactacttga gttatttgag aattagttaa atccatttta attttttcta
     6301 atatatataa aattaaaagt ttgtgatagg ctaattcttc agacgagttt tcaaacataa
     6361 atatcatcct ccgtcaaact atttctatat ataatatatt aaactaattt gtacaaaatt
     6421 aaaagaacaa tttgtattgt attattaatc tttaataatt catataaaaa caattttata
     6481 aataaatata gaaaaacagg gctgctaatt agcagccctg ttgataccca ggtgtcaaaa
     6541 tttactattt attttccagc catttgtctt tcagccattt caactaattt cttagtcata
     6601 tatccaccaa catatccatt ttctctagct gttaagttac ctttgtctat gttttcatag
     6661 ttagataaac ctatttcgtt agctatttct aatttcattt ggtttaaagc agcttttgct
     6721 tctggaacta ctgttctgtt tgaatttgac atagtaaatt tcctcctcaa ataattagtt
     6781 tatgatgtct aacaacactt ttatttatgt gatgttaata ctaatttctc caaaataaaa
     6841 ataatatata cagaaaaata aaggtaaaaa tgtaaaaaat ataaaaaaat taaataaatg
     6901 agtattttag agagtttaat taggtcaata taggttttat ttttttagtt attgtcttat
     6961 aaattaaata ttacagtact tttttgagct gaaaaaaaat aaattttaaa tatagaagaa
     7021 attatttcaa agggggaata gtatgaaaag agaagttaaa aggactaaag ctttgatata
     7081 tagtgtttta tttttttcat tattattagt gttaatattt gtttttgttt atggaataaa
     7141 atacatatgt tctaaatctg acaattcaaa aatgcctata tataaagttg atacaaatga
     7201 gaaaaaaata gctttgagtt ttgatgtagc atggggaact gataatatgg atgatatttt
     7261 aaaaatttta gataaacata atgtaaaagc gaccttcttt ttagttggta gttgggtgga
     7321 tgataatgaa gaaattgtaa aagctataga tgaaaaagga catgaaattg gaaatcattc
     7381 aaatactcat gccaatttaa aggaaatatc aaaagaagac ataacaaaag agatagaaac
     7441 tacatcagaa aaaatatata atataacagg aaaaaggact aatttattta gacctccttt
     7501 tggggacgta aacaataaag ctatggacat ttgtagtgat ttaggttata aagtaattaa
     7561 gtgggatgta gattctatag actggaagga gcttggacca aatcatgtaa ttgaaaaagt
     7621 aataaaagaa tctcaacctg gttcaatagt tttattccat gctaatataa atgatgtaga
     7681 taattatttg gatactataa taagtcgact aaaaaaagat ggatacagtc tagtcaaaat
     7741 atctgatttg ttgtataaag ataattatgt agttgattcc aatggagtgc aaaaaactaa
     7801 gaattaataa gtaataaatg tatattttaa gtaaagttgt gataaattat atagtatatg
     7861 ttagaaaagt gtcgataaaa agacggtgtc tattttttga cactttttat acatgtatgc
     7921 aatatcaata attatagatt aaattgatta aaaaagtgtc taataattga cttttttttg
     7981 atgggtttaa aaatttagaa gaattaatga atgctgtaag acagtatttc caaagggtat
     8041 aagtttgttt aaaagttggc atagaaattg ctttgttaat aagcgatatg ttctatagaa
     8101 ataaacaata tgttaagacc ttataataga aaaatagcat taacattatt tgaacagctt
     8161 ataaaaaaat taatatactt aagggggcgt actagttgaa caaactatat tctattttat
     8221 taaaacaaca cgttggtggt ccagataagc cagtagtaag tgttggagat gtagtaaaaa
     8281 aaggaactct aatcgcagaa ccaactggat taggggcaaa tatttatgcc agtgttagtg
     8341 gaaaaataag tgaaataaat gaccaagcga tagtaattga agctgatgaa gtacaagaag
     8401 atacttttga accattaaaa ggagaaggta tacttgactt aattaaagaa gctggagtag
     8461 taggaatggg tggagcagga ttccctactc atgtaaaatt aaacatagat ttaaatggtg
     8521 gaacaatatt agcaaatgca gctgaatgtg aacctttatt agctcataat ataaaagaaa
     8581 tagaagaaag accagaaata gtttatcaag gtataaaata tgctatggaa gttactaatg
     8641 ctggaaaagg tatgctagca ataaaaagta agcatccaaa agcaatagaa gctttcaaaa
     8701 aagtaataaa gccaggagac aacatagaag ttgcagaact agttgatatg tacccaatgg
     8761 gagaagagag agcaatagta agagatgttc taggaaaatt acttgagcca actcaattac
     8821 cttcagaggc taatgcagta gtaataaacg tagaaacact aactagaata gtagaggctg
     8881 ttgagcaaaa aagaccagtt atatctaaaa atataactgt agttggtcaa ttaaacagtg
     8941 gaaaagaatc tatagtgttt gaagatgtgc caattggaac aactgtcgga gaattaattg
     9001 aaagagctgg tggaataaaa ggtgaatatg gtgaaataat cctaggagga cctttcactg
     9061 gtaaagctac tactttagat gctccaataa ctaaaactag tggtggtata atagttacta
     9121 tgccatttgt taatgaaaag agaaaaatgg gtcttttagt ttgtgcatgt ggacctaatg
     9181 aagaaagaat gagagatata gcaactaaaa tgggagttac tgatatagta tcagttcaaa
     9241 aatgtaaaca agctcaagaa attaaaggtg cattaaaatg tgaaaatcca ggacactgtc
     9301 caggacaagc tcaaaaatgt attgaattta agaaagctgg agcagaagtt atattaatag
     9361 gaaactgtac tgactgtagt aacacagtaa tgggttctgc accaaaatta aaattaggtg
     9421 tatatcatat tactgaccat gtaatgagaa cagttaatca cccattaata agaagaataa
     9481 aaggtgacgt aagattaaaa ataaaataat agataaatag aagttagaca tctttagaag
     9541 taaaacttaa aagttacttc taaggatgtt ttttaatgta ttttgagggc aatatatata
     9601 ttgtaaaata aatggaaaaa atagacaaaa tatgctagat attgtataat gataacaata
     9661 gctattacaa ttaatattgg acaaaaataa tgatactcaa gtatgtggtt tgtattaata
     9721 tgcatattat attaagtcaa ctatccgata gagtaaaagg tttttgtgat atttgaagat
     9781 actctatagt atctttttta ttataaaaac taatttttgt cttttattag ataagtatct
     9841 aggatactaa tttatatttt attgagttgt taaaaaagtg cacttaaaaa gttaaaaaag
     9901 aaaaattaaa aacctattta tagaaactgt gaagtatatc ttaaacctgg gcacttaaaa
     9961 gatatatgga gttagtagtg caacctgcta taaatataaa taggagtaac ttttaattgt
    10021 caaaaatata taaaataaat acataaataa gtaattaata gaagatagat ataaatatat
    10081 ttttgaaatg ctccctgcat tcataacgag tgtagagtgg cagatatctg cccaaaactc
    10141 ctttaattgc tggaagttcc taaagctaac taaaccacaa cataatcatg aaataaggat
    10201 aagtgtgata gagtgtgcat ttaataagca cttgaggtta cgaaagtaga aaaaattagt
    10261 tagatagcat aaggttaaat cctaagtgcc ttgaaaatgg ataatcagca ggtaagcttc
    10321 gaaaagaaga aacttcaacg actagagaga aatctcgtac ctacaagtgt ggggaaatgg
    10381 ggagtaccta atttatggtt aggtaatgat atagtctgtg cttaatagaa atattaagaa
    10441 gttcatagga gaactggtta ggagtaacga ccctaattga acacactaaa gatgtattat
    10501 tgaataagat tcatataata tttaataatt ttaattatat taagtgaata caatatatgt
    10561 ttttagtatc aggagaattt tgaaaacaaa aattaaaaaa tcaagtataa tcagtttagt
    10621 aattgctttt agtatggtat ttactgcatt tacaccaata gtatcttatg ctgacgaagt
    10681 gactagcaat gatacaatac ttaatggaga agaacaatca aatggtcaga catcagatgt
    10741 agagaaacca tcagacggtc aggtaccaga tggggagaaa ccatcagaca gtcaagtacc
    10801 agatgtagag aaaccatcag acggtcaggt accagatggg gagaaaccat cagacagtca
    10861 agtaccagat ggggagaaac catcagacgg tcaggtacca gatggggaga aaccatcaga
    10921 tggtcagata ccagatgggg agaaaccatc agatggtcag ataccagatg gagagaaacc
    10981 atcagatggt cagataccag atggggagaa accatcagat ggtcagatgc cagatggaga
    11041 gaagacatca gatagtcaga cgccagatgt agagaaacca tcagatggtc agatgccaga
    11101 tggggagaaa ccattagatg agcaaactcc agaagaagaa aaaccattgg aagaagaaat
    11161 agttatagag gaaatgagtt taaaacaaga tattgataaa attcttgata tgactttatc
    11221 tcaaataaat aaaatagtat ataacttttg ggaagatgaa gaagatgtaa aggcagatga
    11281 acaatcagaa attaaccaag tttttacttc agaagactca tttattagct tatggtatga
    11341 caaaaaagca aaagtaaaaa attcttgttt actaaaagaa atagatggtt cagatagacc
    11401 ttatcatgat ttaagatttg atgatattac aaagacttta acttttgact atgtaataaa
    11461 gggtcttgta ggagcaagct caaaagacga caaatatgta atagaaggtg aagatggaga
    11521 gcaaactgca ttttgttaca ataatcattt aagaccacca agtagtaatg gaaagagtcc
    11581 atatttacca gcagaagatt ttaatggtca agaaaaccaa aatgaagaag cagttaaatc
    11641 tatactatat gcaggttctg aatttgatgg atttggatat aagcaacaat ttaatttagg
    11701 tggagaagaa aatgaattga tgacttactc agctactcaa agtgcaattt ggattattct
    11761 tggacaaatg gatgaacaag aaagacttaa gcagtatcaa ggttctataa acctttgtga
    11821 taaacttata gaaagagcaa aaactgaaga ggaaagagct gagtatcaga agaagaaaga
    11881 agtagcgata aatataaaag cttatttaga agcattgtta aaagcaggtc gtgaagagtt
    11941 aaagccaaat aatacaggaa aacctagttt gagtaatggt ttaactaaga ttaactttga
    12001 aaaaaatgaa gatggtactt atgaaacaga agctgtagcc ttagtaggtt atagtggtgt
    12061 agtaaagtta aaactaccag atggagtaac tgcttatgat ggagatggta atataatagg
    12121 tacaggcgaa gtagaaattt caacgcaaca gaaatttaaa cttaagagtg ttggaaaacc
    12181 agatgccaaa gctaatattt ctgctgtatc ttatgattat atttttccaa aagctattca
    12241 atattataag gcagtgcttg atttaggaca aaaagaccat tcatcttcat taccatctag
    12301 caagcaaaat ttattaagtt atacaataga gaaaaagaat ggtcaagaag ttaactttaa
    12361 tataggttta ccaacagatg atgatgatgt tgtaaatcca ccagtgccac caatagatga
    12421 tgatgttgta aatccaccgg tgccaccaac agatgataca aatggacata agccaaaacc
    12481 atcaccacca atagatgata cagtaataaa tccaccagta ccaccaatgg atgatacaat
    12541 aataaatcca ccagtaccac caacggagga tgcagtgtta aatccaccag tgccaccaat
    12601 ggaggatgca gtgctaaatc caccagtgcc accaacagag gatacagtgt taaatccacc
    12661 agtaccatca gtaagtgata cagtggaaaa aactccagaa ctatcaagag atgatacaat
    12721 aataaaatca ccaaaaacag gtgatgaaac tcagctaatg tcatatgtat ttattagtgt
    12781 cattgcaatt tgtggattag cgtatcaatg taaaataaaa agaaattaat ctagattatt
    12841 attaagttta aaataaaaag tctcaaattg atttataagt cattttgggg cttttttatg
    12901 taaatataat atgcaaatat agtttgttta aaaatatcta ttttaattat attcttaaat
    12961 ttattattgg atacaggcat tcattaattg tttgatgaat agaaaaatgt tttgaaggtg
    13021 aaaatagtat tgaaaaaata atatactaag ggtataattc atttgatttc atttgtataa
    13081 atttaattat aaattttaaa gtgattatat aaatttaaat gctaattact taataaattt
    13141 taataataat tatttaataa ataattatta taatatgcta gaataaatat atataaataa
    13201 gttttgatga ggtactacta taaagggggg actttatgtt ttcgatacca gaagtaaaaa
    13261 aagtagaaga ggtaatggat acaaaattta caactataga cgaagacaca agaattgaag
    13321 atgcaataaa agaaatgata aaaagcaaca caaaaactct aatggtaata gattcatctg
    13381 accaattaaa aggcattata tccatgacgg atatacataa tctttatgag atgcataaaa
    13441 agtatgaagg tcaaccagta aaacttataa tgaagaagga tgtaatatat gtcaacgaag
    13501 gtttaacctt agatgaatgt agagatataa tgatattaaa aaatataggg attcttcctg
    13561 ttcttagaga taataaaatt attggagttc taaaacaaga gcacattagg gactatctat
    13621 atatgcattt agaagattat ggattaactt tgaagtatat tataggtcaa attaaagaag
    13681 gaatttgtgc tataaataac gaaggtgttg ttatcttgtg gaataagttt atggaggaaa
    13741 ggtatgatat aaagtctgaa gatatagttg gtcgaccgat gaatgaattt ttggaaaata
    13801 ctatttcaga aaaagtacta aatagtaaag ttggaatgag tgacttgtat tttacagata
    13861 aaaaagaaaa tatgtatgca ttagttcatg caaatcctat attttataaa gaagaattta
    13921 tcggtgttgt atgtacagaa gttgatgtta cagaagctaa gatattagct cttgaacttg
    13981 aaaaagtaaa tgatacatta aaatatctaa agaacgaagt caaaaattta tctaaaggta
    14041 gttttgataa gattttaggt aaaagttata aattggaaaa atcgaaagcg attgcaaaac
    14101 aagtggctag aacaaatagt agtatattta tttggggcga aagtggtaca ggtaaagaag
    14161 tatttgcaag agctattcat gattatagtg agagaaaagg gcaatttata cctgttaact
    14221 gtagtgccat accaaatgag ttatttgaaa gtgagttttt tgggtatgaa tcaggtgcat
    14281 ttacaggagc aagtaaaaag ggtcgaatag gtatatttga acttgcaaaa gatggaactg
    14341 tatttttaga tgaaattgcc gatttacctc ttagtatgca agcaaagttg cttagagtat
    14401 tacaggaaaa agaaataaga agagttggtg gagataccac aataaaaata aatcctagaa
    14461 taatatcagc taccaataaa gatttgagta aaatggtaaa ggcagaaaaa tttagagaag
    14521 atttatacta tagattaaat gttgttgaaa taaagattcc tccacttaga gagagaaaag
    14581 aagacatagg attattggtt catagctttt tagaggagat atgtaaacaa aataataagc
    14641 ctgttcttac aattagcaaa gatgtaatag atatatttca gaattataga tggaaaggta
    14701 atattagaga attaaaaaat acgattgaaa atattgttgt attgtcacaa aattctaaaa
    14761 tagatgtgga tgatgtacca agttatatga tggattctac aaataatagt actgaagaag
    14821 aagagtatcc tttagattta acgaaggcaa ctcaaaaaat tgaaataaag aatataacca
    14881 aggcacttaa aatgtctaat ggaaataaag ctaaagcagc taagatatta aatataccca
    14941 gaacaacttt gtattataag atagaccaat acaaaataga tgtgtctaaa atatgacaca
    15001 tgactaaatt ttgacaaaat gataaacttt aatttaaaac ctagtaaagc caacacttgt
    15061 ataagtgttg tgtatataga tgtgttaaaa aaaatgaaaa agtgttgatt aattgacact
    15121 ttttttgttt gagaaaatgt ttacaaatac aaaaaataac atattttaaa taaatattta
    15181 tataatattt ggctaaaaat agcgatagat gaagaatcta ttatagaaaa aatctttttg
    15241 ttaataagtt ggcataggaa ttgcttataa tataaagtga ctttaagtga gaaaaaaata
    15301 atattaatca taacaaatta tgtaggtttt aatttactta atttctcagt taaggggtta
    15361 cctgttgagg aattgagatt taaaataata aataagaaaa taatgtaagg aggtatttta
    15421 tactatgtca ataactttag aaacagctca agcccatgca aatgacccag cagtttgttg
    15481 ttgtagattt gaagcgggaa caattatagc gccagaaaac ttagaagatc cagcaatatt
    15541 tgcagactta gaggattctg gattattaac aataccagaa aatggattaa ctataggtca
    15601 agtactagga gctaagttaa aagaaacttt agatgcactt tctccaatga ctacagataa
    15661 cgtagaagga tacaaagcag gagaggctaa agaagaagta gtagaagaaa cagtagaaga
    15721 agcagctcca gtatcagaag cagcagtagt tccagtaagc acaggagttg caggtgaaac
    15781 agttaaaata cacataggtg aaggtaagaa cataagctta gagatacctt tatcagtagc
    15841 tggtcaagca ggagttgctg ctccagtagc aaacgttgct gctccagtgg caagtgcagc
    15901 agcagaagta gctccaaaag ttgaagaaaa gaaactttta agaagcttaa ctaaaaaaca
    15961 ctttaaaata gataaagttg aatttgctga tgaaactaaa atagaaggaa ctactttata
    16021 catcagaaac gcagaagaaa tatgtaaaga agctaatgaa actcaagagt tagttgtaga
    16081 tatgaagtta gaaataataa ctcctgataa atatgaaact tacagtgaag ctgtattaga
    16141 tatacaacca atcgctacta aagaagaagg cgaattaggt tcaggtataa ctagagttat
    16201 agatggagct gtaatggtat taactggtac agatgaagat ggagttcaaa taggtgaatt
    16261 tggttcttca gaaggtgagt taaatactac tataatgtgg ggtagaccag gtgctgctga
    16321 caaaggtgaa atattcatca aaggtcaagt aacaataaaa gcaggaacta acatggaaag
    16381 accaggacct ttagctgctc accgtgcatt tgactatgta actcaagaaa taagagaagc
    16441 attaaagaaa gttgacaact ctttagtagt tgatgaagaa gtaatagagc aatacagaag
    16501 agaaggtaaa aagaaagttg ttgttataaa agaaataatg ggacaaggtg caatgcatga
    16561 taacctaata ttaccagttg agccagttgg tacattagga gctcaaccaa acgttgactt
    16621 aggaaacatg ccagttgtat tatctccact tgaagtatta gatggtggta tccatgcatt
    16681 aacttgtata ggacctgcat caaaagaaat gtcaagacat tactggagag agccattagt
    16741 aataagagct atggaagacg aagaaataga tttagtaggt gttgtatttg ttggttctcc
    16801 acaagtaaat gctgagaaat tctatgtatc taagagatta ggtatgttag ttgaagctat
    16861 ggaagttgat ggagctgtag taactactga aggtttcgga aacaaccata tagatttcgc
    16921 atctcacata gagcaaatag gtatgagagg tataccagta gttggtgtaa gtttctcagc
    16981 tgttcaaggt gctctagttg ttggtaataa atacatgact cacatggtag acaacaataa
    17041 gtctaagcaa ggtatagaga atgaaatatt atctaacaac actttagctc cagaagatgc
    17101 tgttagaata atggctatgc ttaaaaatgc tatagaaggt gtagaagtta aagctcctga
    17161 aagaaaatgg aatccaaatg ttaaattaaa taacatagaa gctatagaaa aagttacagg
    17221 agaaaaaata gtattagaag agaatgagca atctctacca atgagtaaga agagaagaga
    17281 aatatacgaa aaagacgaaa actaaaattt agatactaac taagatttag acaataacat
    17341 aaatttagat ccaagtttaa aacagtatag agaggtagaa ttataatgga taggttaaaa
    17401 tatatctcat cagagacttt ttatgaaggt attatagttg acatcaaagg cggtggagtg
    17461 actatagacc taaaaggtag acttggacaa tttaaaatac ctaatagaat gcttataact
    17521 gactacgaac ttaagatagg tcaagaagtt ggattcatgc taagctatcc tgaggtatta
    17581 agtccagagc ctaatcaaga gtatgtagaa aatattagga gagaaaaaga aaaacaagca
    17641 gaaatgcaat caaaacaaga agaaaaataa taaataatcg tttgaaaata gaagggagag
    17701 gaaatatatg agccttacaa cagtacaagg acttcaatct gaaatattcg ttccaataac
    17761 acctcctcca gtttggactc ctgtaactaa agaattaaaa gatatgacta tagctttagc
    17821 tacagcagct ggtgttcatt taaaatctga caagagattt aatctagcag gagatactac
    17881 atttagagct atacctaata cagctacagt tgatgaaatg atggtatcac atggtggata
    17941 tgacaatgga gatgtaaaca aagatataaa ctgtatgttc cctatagata gattacatga
    18001 attagctgca gaaggattta tcaaaggagt agctcctatg cactatgcat tcatgggtgg
    18061 tggaggaaac caacatgtct tcactgaaga aactggtcct gctatcgctg ctaaacttaa
    18121 ggaagaggga gtagacggtg tagttatgac agctggctga ggtacttgcc atagaactgc
    18181 cgtgatcgtg cagagagcaa tagaggaagc tggaatacct acaataataa tagcagctct
    18241 tcctccagta gttagacaaa acggaactcc tagagcagtt gctccattag ttccaatggg
    18301 tgctaatgct ggtgaaccac ataatataga aatgcaaaca catatattaa gagatacatt
    18361 agagcaatta gttgcaatac catctgctgg taagatagtt ccattaccat acgaatataa
    18421 agctcacgtt taaaatgtaa tttatatttg aaaatataga acccttctcc tacacggtta
    18481 ggagaggggt tttaagaaca aaggtggata ttatggaaga aaaaataatg agaagacttg
    18541 ttataaaacc gtttcatatg aatgaagtta atttcggttc aaaaacttct ataaagaaag
    18601 atgtacttac aatagattta tcaagtatag atgaaataaa agaaagagaa gagttaataa
    18661 cagacataaa agtagacatt ataaaacctg gagattatga cagagaaatt aatactatta
    18721 tggacattat accaatatca acaaaagttt taggcagact gggagagggt attacacata
    18781 ctttaacggg tgtatatgta atgttaactg gtgcagatga agatggaaat caaatgcatg
    18841 aatttggttc ttcagaagga attttgaaag accaaatggt atttgggaga tatggaactc
    18901 catcagttga agattatata atccatgtag atgtaacttt aaagggaggt ttaccttttg
    18961 aaagaacact tccacttgca gcattcagag catgtgatga ttttatacaa gaaataagag
    19021 cgtctttgaa aatggaagac ggaagaaatg ctactcaagt tcgtgaatat tttgacaaaa
    19081 ttcgaccaaa tgcaaaaaag gttgtcatag taaaacaaat agcaggtcaa ggtgctatgt
    19141 atgacaatca gttattctca aaggaaccta gtggatttga aggaggaaca tcaattatag
    19201 atatgggaaa tgtacctatg attatatctc ctaatgaata cagagatggt gcccttagag
    19261 ctatgactta agagaggtgg atattatggg aataggacct tcaacaaaag aaacatctct
    19321 tcatcacttt agagacccac ttttagacat agttgaaagt gataaggatg tagatttact
    19381 tggagtaata gtagtaggaa ctccagatgg aaatgaaaat aaaacctttg tgggacaaag
    19441 aacggccgct tggctggaag ccatgagagt agatggagct atagtttcat cagatggttg
    19501 gggaaactca catgttgact acgctaacac atttgaggaa ataggaaaaa gagatatacc
    19561 tgtagttgga gttacattta atgggactca agctaagttt gttgtaagca atcaatatat
    19621 ggataccata gttgatatga acaaatcaaa agaaggtatt gagacagaag tagttggaga
    19681 aaacaacaca aatgaaatag atgctaaaaa agctttggca tttttaaaac ttaaaatgag
    19741 aaaacatgga taaaaaattt aagtaaggga atgagttttg tgggaatttc tacatcaatg
    19801 aaaaacacat cacttcatat atttagagac ccgttactag atttgataga taatgattct
    19861 gacatagact tgttaggagt ggttgtagtt gggacatcag agcacaatga atggaaaaca
    19921 tttttagcaa ctagacttgg aagatggatt gaatcattgc gacctgatgg agtgattata
    19981 actttagata tggcaggaaa ccagcacatt gattttacaa atgctatagc agagtttgta
    20041 aaatcagata tacctacagt aggtcttact attatgggag cagatggatt agtaattacc
    20101 aatccatagt tggataaagc taccataatt gattataaaa agacaacagg ctacatagaa
    20161 acagaagtag ttggagacaa tcacatggat gaagttgatg ttaaaaaagc attggctttc
    20221 ctaaaattaa aaatgcgtaa agacgcaaaa taaacctaag ggggaatttg atatgaaatt
    20281 tagcagaagt atacaagcta tagactctca tacagcaggt gaagcaacta gaatagttgt
    20341 aggtggaata cctaacataa aaggaaattc tatgcctgag aaaaaagaat atttagagga
    20401 aaatctagat tatttaagaa ctgctataat gttagagcca agaggacata atgatatgtt
    20461 tggttctgta atgactcaac cttgttgccc agatgctgac tttggaataa tcttcatgga
    20521 tggtggcgga taccttaaca tgtgtggcca tggtacaata ggagctatga cagcagctat
    20581 agaaacaggt gtagttccag cagtagagcc tgtaactcat gtagttatgg aagctccagc
    20641 aggaataata agaggagacg ttacagttgt agatggaaaa gctaaagaag tatcattctt
    20701 aaacgtacca gctttcttat ataaagaagg tgttgaagtt gacttaccag gtgttggaac
    20761 tgttaaattt gacatatcat ttggtggaag cttctttgct ataatacatg caagtcaatt
    20821 aggtttaaaa atcgaacctc aaaatgctgg taaattaact gaactagcta tgaaacttag
    20881 agatataata aatgagaaaa tagaaataca acatccaact ttagctcata taaaaactgt
    20941 agacttagtt gaaatatatg atgagccaac tcatccagaa gctacttaca aaaatgtagt
    21001 tatatttggt caaggtcaag ttgacagatc tccatgtgga actggaacaa gtgctaaatt
    21061 agctacttta catgctaaag gtgaattaaa agtaggagaa aaattcgtat atgaaagtat
    21121 attaggaact ttattcaaag gtgaaatagt tgaagaaact aaagttgcag atttcaatgc
    21181 tgtagtacct aaaataactg gttctgctta tataactgga tttaatcatt ttgtaataga
    21241 tgaagaagac ccacttaaac atggatttat tcttaaataa gtaataaata aattaggaaa
    21301 ttaaaataga atagtagcca cttgtcggtt gctattctat agcttttagg ctataagatt
    21361 cagaatatat gaaaaaataa aaaaatagga ggatggatta tggtagcaat cttaaaggca
    21421 ttatatgtag tattcgcagc aatctttgca gttgtgtttg gtaaagacgt agctaaagca
    21481 aacaaagagg ggaaactaga agaacaaagt actccaaaag ttgctgtaac aggatttata
    21541 acagacttct ttgatacatt aggaataggg tcatttgctc caacaatagc aatgtcaaag
    21601 gctttaaagt taaatatacc agataaaaaa atgcctggta cattaaacgt agcacatact
    21661 attccagttg ttacagaagc attcatattt acatcaatca taccagttga tggtgttact
    21721 ttagtatcta tggttgtggc agcagcagtt ggttcatata taggtgcagg aataatagct
    21781 aagatggacg agagaaaaat acaacttgtt atgggtgtaa ctttagcatt aacagcaata
    21841 ttaatgttat taggtcatcc ttggattaac gtattaccag gtggaggaaa tgaattaggt
    21901 cttactggtg ttaaattagt aataggtgta ataggtaact ttatactagg agctttaatg
    21961 acagctggta taggtttata tgctccaggt atggcaatgg tttatttctt aggaatgtct
    22021 gcaaaagttg cattcccaat agttatgggt tcttgtgcat tattaatgcc agttgcaagt
    22081 atgaaattca taaaagaaga tgcatatact aaaaaagcat cagttataat agctatatgt
    22141 ggtttaatag gagttttcgt agctgcatat atagtaaaaa ctctaccaat ggatatatta
    22201 aaagcattag taatagtagt tatagcttat acttctgcta caatgataat ggctgcaaat
    22261 aaaaacaaaa agactgctta gttaaagact acttagtgtt tattaaatat tacagctaat
    22321 aataaagatt ataaactgct ttaaaatatt gaattaatat tttagggcag ttttttattt
    22381 actaaattaa tataaagcaa ttaacatgcc aattatgtaa tatatatagt gatttagtag
    22441 atttaatgta aaaaacgttg tatttttaaa gaataaagca taatttaaag ctaaatattt
    22501 aaattttata atcaataagt tcaa
//
LOCUS       DAMJWU010000053        22439 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMJWU010000053 DAMJWU010000000
VERSION     DAMJWU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.053"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(392..532)
                     /locus_tag="PC460_002242"
     CDS             complement(392..532)
                     /locus_tag="PC460_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601125.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(790..1950)
                     /locus_tag="PC460_002243"
     CDS             complement(790..1950)
                     /locus_tag="PC460_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF2601126.1"
                     /translation="MKLNKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGSVSAVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSISDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(2121..2324)
                     /locus_tag="PC460_002244"
     CDS             complement(2121..2324)
                     /locus_tag="PC460_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF2601127.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(2411..3445)
                     /locus_tag="PC460_002245"
     CDS             complement(2411..3445)
                     /locus_tag="PC460_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF2601128.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTATSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKEAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSKFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            complement(3967..5328)
                     /gene="rlmD"
                     /locus_tag="PC460_002246"
     CDS             complement(3967..5328)
                     /gene="rlmD"
                     /locus_tag="PC460_002246"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2601129.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIIVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(5355..6077)
                     /locus_tag="PC460_002247"
     CDS             complement(5355..6077)
                     /locus_tag="PC460_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2601130.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(6077..6406)
                     /locus_tag="PC460_002248"
     CDS             complement(6077..6406)
                     /locus_tag="PC460_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF2601131.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            6736..7662
                     /gene="fba"
                     /locus_tag="PC460_002249"
     CDS             6736..7662
                     /gene="fba"
                     /locus_tag="PC460_002249"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF2601132.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(7797..9428)
                     /locus_tag="PC460_002250"
     CDS             complement(7797..9428)
                     /locus_tag="PC460_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2601133.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(9911..10948)
                     /locus_tag="PC460_002251"
     CDS             complement(9911..10948)
                     /locus_tag="PC460_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2601134.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(10967..11752)
                     /locus_tag="PC460_002252"
     CDS             complement(10967..11752)
                     /locus_tag="PC460_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2601135.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(11785..12918)
                     /locus_tag="PC460_002253"
     CDS             complement(11785..12918)
                     /locus_tag="PC460_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2601136.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(12966..14093)
                     /gene="hadC"
                     /locus_tag="PC460_002254"
     CDS             complement(12966..14093)
                     /gene="hadC"
                     /locus_tag="PC460_002254"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF2601137.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(14093..15319)
                     /gene="hadB"
                     /locus_tag="PC460_002255"
     CDS             complement(14093..15319)
                     /gene="hadB"
                     /locus_tag="PC460_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF2601138.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(15334..16134)
                     /gene="hadI"
                     /locus_tag="PC460_002256"
     CDS             complement(15334..16134)
                     /gene="hadI"
                     /locus_tag="PC460_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF2601139.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(16173..17372)
                     /gene="hadA"
                     /locus_tag="PC460_002257"
     CDS             complement(16173..17372)
                     /gene="hadA"
                     /locus_tag="PC460_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF2601140.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            18049..19047
                     /locus_tag="PC460_002258"
     CDS             18049..19047
                     /locus_tag="PC460_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2601141.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            19409..19936
                     /locus_tag="PC460_002259"
     CDS             19409..19936
                     /locus_tag="PC460_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF2601142.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            19963..20559
                     /locus_tag="PC460_002260"
     CDS             19963..20559
                     /locus_tag="PC460_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF2601143.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(20624..22207)
                     /gene="asnB"
                     /locus_tag="PC460_002261"
     CDS             complement(20624..22207)
                     /gene="asnB"
                     /locus_tag="PC460_002261"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2601144.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         6954 a         4071 c         2676 g         8738 t
ORIGIN      
        1 attaaatttt taatttaagt aatagtatat gtaatcttta gtcatcttct taccagtttt
       61 ttatccactt ttctaatgaa gttatttttt catatgttaa ttcctcttga aataatgata
      121 ctaaaagact ttttataaaa ttataactta aaagtttatg tatttttttg atatgaattt
      181 atagtactta tcttctttaa ttgagattgt gtatatttaa ctaaacatta caacaataat
      241 tctatgaaat caaatcaatt atttacatat ttttttagaa caataataag tatatattca
      301 caaaattttg tgttaatatt tttaatttgt gatataataa aagcaaggaa acatatttta
      361 cttaactttg agtgatgttt ccttcaacaa attattttga agatttactt ttctggaact
      421 ttatattaag ttcccagcca ctcttagttg gtcgctcaga gtggctcttt aatttatcgt
      481 aaatgtaact ggctataaca ccagttataa tactcatcat tagattttcc atagttttca
      541 cctcctctca taagaaagtg aagaaacttc catggaaaca ccactcatag ctttttcaaa
      601 tatttattcc ttgccatatt aattatatca taaatattgg aaacataaaa ttttaatact
      661 tatcgtcttt attattattt tttataatta ataatttttt ttaataatac ataatataaa
      721 tttatatttc ttcaataaaa aagacaatct taattaattg tcttttttaa tatatatttg
      781 gctaatacat tattttacac aaacattaca agggtctatt ttaccttgtg acttagcttg
      841 ttttaatgtt actttattta ttgttttact tctttttagt gtagaacaat tttttgttag
      901 atgatatgat tttccattag gagttacata tactgtactt ttatcagtta atccaggatt
      961 attagagcta ggctcattac ttggtttgtt cgtattattt gaattagtat cttttacaat
     1021 gcctgtattt gaatccccaa cattattagt tatatttaaa cattgtttta taacaacatc
     1081 atcaatattt ccaatagcag ttattttact tgcatttttt aatatactct tattacttcc
     1141 atttccaact aacactatag gtgttttttg tgcaattgta gaaccaacta aagaataaac
     1201 taaatcagtc ccacttgcta tgtaaaattc ttttgagcca ttataaaatt tatttattat
     1261 ttgtttattt gtatcgtatc tatctactcc acctattctt gttgctttag tttcatttat
     1321 taacttatca ctaatactga cttttccacc aattgcataa gtttctaatc catctgtagc
     1381 aaaacttgta gtgtttccat cagttaatac tattggagat ttatctctaa ctgctataga
     1441 agcaacactc atagcatctg gttcaccttt aaaagcatta gttaatatta cttttttaac
     1501 agcacttaca cttcctattt cttttgcaac tttatgactt gtatctattc tatcagttcc
     1561 acttattcta tttatgctta tttttttatc acttaatatt ttctgaactg atgctcctat
     1621 cgaatttttt cctccgatta gatatacttc ttttaccttt tctaatcttt ttaatgtttc
     1681 tgaagggatt gtatctttct tagttaacaa tattggggca tttttagctc ctgacaatcc
     1741 acttgctgac aatccatcag ataatccatt ttcagaatta ataagtattg caatatcata
     1801 attttgttta tctgctatta aacctgctgt ttcatactta tttgtacctt ttattgaatt
     1861 aatagaacta agagcgtttg atgttggaca actagcaatc aataatgaaa cagacatccc
     1921 tattgccaat agtgttttgt ttaatttcat aatgaaatcc tcctataaat tttataatat
     1981 tacttaaata ttataattct acataaaaga atattttcct ttatgaaatt agtttatatt
     2041 tcaaaatttt gtcacttttt aaatctatat actaaaaaat agactatttg taacagtcta
     2101 tttcttagta tatcaaatat ttatttaggt ttacgtttct ttgtactagt ttttgtatat
     2161 gatattccag ttccaggtat actcaatgta gctgattttc ttccttttga attaactgac
     2221 actctagcac ctttaacacc agcacttata cctacactct tcttacctaa atttaatttg
     2281 agtcctcctc ctagatttat gcttttccta aagttaaatc ccatagatat aaccttccct
     2341 tctatatttt aaatatttat acttaaaatt atatttgtta tacacttgcc atcattggta
     2401 tatctagcat ctatatcttt ttacgttttt tcttcttata tactactcca gcaactacag
     2461 ctgctgttcc agctccaacc ccataagtag catttctact tttctcttct tctctttttt
     2521 ctaaaccttc atcatataca ccttttaact tagtttcaat agacttcatt tcatctggta
     2581 tactaaaatc ttcttcattg taaccttgat tatatacact ttcttttgct ttaggtaact
     2641 taaccttacc attatcatat ccttctttaa accaattagc atatttaaca ttacctatag
     2701 ttgatgtgtc aaatctttct cctttgatag cactctcaaa tccttgagta gtgtaatcat
     2761 tttttaattg ttcttgtatt tcgtcataag cattattata agcatctata aatttagaat
     2821 ctatgttttc ttcaaatgtc attttttctt tatctttatt gctatcctct atccctttag
     2881 ctttatattc ttctatcttt ttagtcttat attcattgta tccagttgaa tatccggtat
     2941 cataagcatt ttttaaaact tctttttcat atgtattatt agatttagca cctgtaagcc
     3001 ctagattata tccagtctct ttagcctcct tttcttcctc ttctaattta gccttacctt
     3061 tttcatatcc ttctgagtac tttgattcat atgtatcact ataatctcca ctataatttg
     3121 atgaactata atttccttta tacccatctt catacccttg attatatcct ttttctccaa
     3181 cagacctctt ttgcttttct tcattattaa ccgaagttgc tgttccagaa ctgcttcctc
     3241 ctccagtata aggacaacca ccattattat gtaaatgagc tggataacca ccacaatgat
     3301 agtgataact tcctaatcca gatttatttt tattatcacg atgacctcct gatgaatcag
     3361 ttcttcctga atgagcatac gaaaagctcc ctacactaat catagttata ctcattgcaa
     3421 cagctattaa ctttttctta aacataatct atttccccct aaaatattct atgattttat
     3481 tatatcagtc aatatattat gtttaaaccg ttaaaattcg acaatgtaaa tatttgatga
     3541 attgtatttg taaataaatc ttttagcata tcttgaatat ctgtagttgt tttgacatca
     3601 tattcttcaa taagtttttt aatatttcgt ttttgttaaa cataaaaagc gtgcactttt
     3661 ctttgtatag tgatatttga tattttaatt atcactactt tagaagaata cacactttat
     3721 tttaaaaacc ctgatactat gttattagaa aatggtacta atataaaaga tatccagcat
     3781 atactcggac atgctaaaac ttcaacaact atggatacat atttacatgt aactaacaac
     3841 atgagaaacc agacggtaaa tatttttgaa atcattgtag aataaatttg ccacccaaaa
     3901 ataattatgg tggcaaatag gtggcaaatc tacttttaag acacactata aaagtctgaa
     3961 accctgttat tctctaataa gtttaactac agtctcacaa tgtggcgtat ttgggaacat
     4021 atccatacac ttaaccatct taatctcata accatacccc ttaaaatcaa ccaaatcaac
     4081 aaccaaagac ttaggattac aagaaatata aacaatctcc ttagcaccaa atccacaaat
     4141 atccctaata gcatccttat gaatgcctgg tctaggaggg tcaacaataa tcaaatcagg
     4201 cttgtccttt aaatccttaa caaccttagc cacatcccca gcaataaact cacaattagt
     4261 aagtccattc aacttagcat tctcattagc agcaacaaca gcttcctcaa ctatctcaat
     4321 accatacact ttcttagcag caccagccat aacctgtcca atagacccag taccactata
     4381 caagtcaaat acaactttat cattgtaatc tccaataaaa tccctagcaa tactataaag
     4441 aacctcagct cccttagtat tggtttggaa aaacgaaaat ggtgaaatct taaacttaag
     4501 acctaaaatt tcttcttgta tataatctct tccatacaat actcttaact catcacattg
     4561 gactgcatct gctaaaccat cattaatagt atgaagtaca cctactaact cagcttttaa
     4621 atcaatccct aaaagcatat ctttaaactc atacatatca aaatcttctt gagaagaagt
     4681 tactatatta actataatct cattagtgtg aatccctttt ctaactacaa gatgtctcaa
     4741 atatcctttg tgattcattg ttctataata aggaagcttt ttttcattga aaaactctac
     4801 actagaaact aatatctttg agaagtcttc atcaaccaac atacactcct caacagtttg
     4861 tatatctata tgctttcctt ttttatgaag acctaatgtc aaaggtccat tttttacttc
     4921 atctccaaaa gtatattcca tcttatttct ataatatttg ttttctggac ttccttctat
     4981 ccctaaaaac tgaaatccaa ataaatcttg ctttaaaaat aaatccataa cttgtttttc
     5041 ttttatttct aattgttttt catatggcac agacaatagt gtacatcctc cacactctct
     5101 aaagtgcttg caagtttctt cagtttcaag tggtgagttc tctaataatt ctatcatctt
     5161 aacttcagct ttcttacttc taactttttt aactccagct ctcacctttt gaccacttat
     5221 tccacccttc atatggataa ctctatctcc aacttggctt aaagatgtac caccaaattc
     5281 cattttatct acttcaaatt ctataacatc ttttttcttc acagacattc ctccaattta
     5341 ctatagtatc tttattatct ttcttcaatc aattttactt cttcatctgt taactctcta
     5401 tactcaccta actctaaact ttcatccaat tttaaattgc ccatagacaa tctttttaag
     5461 tatactactt ttttgcctac gctttcaaac attcttttta cttggtgaaa cttaccttca
     5521 tgtattgtaa gctctatttc tgattcatca tcacttttta atatatttaa ctgtgatggc
     5581 atagttttat atccgtcatc aagcactaca ccttctaaaa aagcttctac atcttcttca
     5641 gtaactactc catcaatttt tgcataataa gtcttaggca catgcttctt aggagataaa
     5701 actctatgag aaagctttcc atcattagtt aaaactaata atccctctgt gtctttatct
     5761 aaccttccaa caggaaatgg ttcaaaagct aagtatgata aatcaattaa atctaatact
     5821 gttgggtcat atttatctgt agttgctgat atatatccat caggtttatt catcatcaaa
     5881 tatatatatt ctctatatat aacttcttct ccattaaata atatctcatc attttcagtg
     5941 tctacttgag ttcctggatt tgaaacttca cttccattta ctactacaga cccttgttta
     6001 caatacttct ttatttcact tctgcttcca taacctaaat ttgaaagaat tttatctata
     6061 cgttgctttt tagccattaa aaatgctctc cattctcata atatttttct ttttcaaata
     6121 ctgtaaatgc taaatccata ctttctacat ctaaattatt tcttatacta tcagttatta
     6181 ttcttgctac tatatcttgt acttcttgtc ctctatcaaa ccaagctact tcaacaaaag
     6241 gatatacatc tgctatttta ccatctctta ttgcaactga ctttatacat tctatttcaa
     6301 agtagtctct tggacatttt acagcttcaa ctaaatcatt tatcattttt tcacttattt
     6361 tacatatatc attttcatta attcctctta tttttatctg tggcatagtt tatctccttt
     6421 attttaaatt tttagtgaat tatcatccac ttaattccct tcgataacgt ttgaagtggt
     6481 aacttcttgg tcaagacaac tattgttatc agtatctcca aactaataag gtagttccta
     6541 cccttatagt tagcattata tagctaattt tcctaaattg tttatattct ttattatttt
     6601 atttaatcct attgtcttct atttaacatt atacttgtaa tttatagtta ttacaattta
     6661 ctatatactt tttttgattt aacgttataa tataagtgat gggtaaattt taaaagttaa
     6721 taggaggagt ttaaaatggc attaattact acaaaagaaa tgtttaaaaa agcttacgaa
     6781 ggtggatttg ctataggtgc atttaacata agtgacttag aacaattaca aggtgtttta
     6841 aaagctgcta aagctaaaaa ttcttatgtt atgatacaag cttctatgtc agctgttaaa
     6901 tatgctggac ctcatacatt agttgaaatg gttaaggctg cttctgatga aataggtgta
     6961 gatgtagctc ttcacttaga ccatggtcca aatatggatg ctattaaaac ttgtattgat
     7021 gctggtttct cttcagttat gatagatggt tctcattttg actttgaaga aaatgttaga
     7081 ataactaaag aagctgttga atatgcacac tctaaaggtg ttgttgttga agcagaactt
     7141 ggtgttctag ctggaacaga agatgatgtt acttctgatg ttcacaagta tactcaacca
     7201 gctgaggcag ttgagttcgt tgaaagaaca ggtgttgatt cattagcaat agctattgga
     7261 acttctcatg gtgctttcaa attcaaagga gaagctaaat taagattcga tatactagaa
     7321 gaaatccaaa gcaaattacc aggtttccca atagttcttc atggtgcatc tgctgttgac
     7381 caaaacgctg ttgctacatg taatgaattt ggtggaaata tagctggtgc taaaggtgta
     7441 cctgtagata tgttaagaaa agcatcttct atggctgttt gtaaaataaa catggatact
     7501 gacttaagac tagctatgac tgctgctatc agaaaattct tagctgaaaa tccaaaagaa
     7561 tttgacccaa gaaaatacat tggtgctggt agagatgcta tacaagctgt agttgaaagc
     7621 aagatagatg atgttttagg ttctgctaac tctataaact aatataactt ttaaatattt
     7681 ataaaagaaa agagatgact taaagtaatt aaatttattt ttaagccatc tcttttaagt
     7741 tgtataaatt atgtatctta taatatttta attatattgt ctttttaaac tgacagctaa
     7801 acgtgaatat gtccactctt tatctttcta tggatggtag aaggatatat acctataact
     7861 tcagcagcct ttgttattga accatactta tttatagcct tacttatcat tatttggtcc
     7921 actattttat atgcatcatt aagattcata attccattta ccactattgg tgataaatca
     7981 tctgtttcat tatcaatcat atctaaattt atcagcatgt taaattcatc ttctccaatt
     8041 acatttttag ctgataatac tacaaatctt tcaatgatgt tttttaattc tcttatgttt
     8101 cctggccaag aatattcttc taataactca ataactttag gcgaaacttc tacttctcta
     8161 ttgtactttt cattgtaaag ttttaagaaa taatttacta atggtactat atcctctttt
     8221 ctttctctaa gaggaggtat ttttactgga ataacactta atctatagta caaatcttgt
     8281 ctaaatttaa ggttatttct aaggtcttca tgagaaatat tagtagcact tatatatcta
     8341 acatctattt ttataggctt actaccacct attctagtaa tagtattttc ttgtataacc
     8401 ctaagtaatt ttttttgcat ctgcattggt aactcaccta tctcatccaa gaaaatagtt
     8461 ccaccattcg cctcttcaaa aagtcccttt ttaccttttt tcttagctcc agtaaaagaa
     8521 ccttcttcat atccaaaaaa ctctgactct aataattcat ttggtatagc tccacagttt
     8581 atagctataa agggtttgtc tctccttgaa ctatacttat atattgttct tgctattatt
     8641 tcttttccta cacctgattc tccatttata aatatagaag aatctgaggc tgcagctctc
     8701 ttaacaactt tttttatatt gtccataatc ttactttgac ttatatactc tgtgtcttct
     8761 gatatatcac tatattttat ctgagattca agcttaataa cccttgaaat atctctccct
     8821 gttagtactc ctcctgtaag ctttccctta gaatcccatc ttggaactgc cgtatatgca
     8881 ataagcttcc catctgggta aacgatattt ctataaatct ttttcttcac tttaagcatg
     8941 tttaatatta ttggttcttc aactaatcct tgtctcacaa ggtctgttat atttctacca
     9001 agtacctcag ttcttttaaa aggtaatatc tcatcactga cagtgttcat cttttcaatt
     9061 accccatctt tgttaaacac aacaacaact tcatctatat gctctattat atcttcaatt
     9121 actttatcag atattagaga cttctcaaat aaataaacta tattattttc attattatta
     9181 tctttcatat tgtatatatg tgcagtcatc cttaactctc tgaaagctat tttcttttta
     9241 ctatctgtca gtatttctgt tttgtcaaaa tttgaatcta attcatttat atttctaggc
     9301 ttttttatat ctagaaaact aaaaatacta tctattagat gattacagta tattatttcg
     9361 cctgattctt tacataaaat ggctggattt tctacatgtt ctataagccc tattaaatta
     9421 ttatccatta atattcgcac ctttcattaa gtaatttatt gaactacata caaaatttat
     9481 tgtttgctta caataaatac catcagcaca tataatcaac tccttataat attcgtcttt
     9541 tcaagtagtt tatttcatta aaacgtaact aagatataat tttaatgatt atagtttgtt
     9601 aaaaatttca ttttgctttt aattgtattt tatcacatat ttagtaatta tttgtctatt
     9661 taatacaatt taattaaatt taagatttat ttaaaaaaac aaagataatc cttacagaat
     9721 ataactcaca atttgtaagc aatatacata taaggattat ccagattatc ttgtattata
     9781 aaattttatt caaacagact ttttaataac tgacaaatac acactagcaa taacttatca
     9841 aagggttaat aattatgtta ctaactaata agacttattc aatcagtaat atttatatag
     9901 tttgtatcta ttaagctttt atagcttcta ataaatcatc aacgctatct aatttttcta
     9961 taagcattgg aactatttca tgtaagtctc caactactcc atagtcagcg atttctaaaa
    10021 ttggagctgc tgggtttttg tttatagcaa ttatgaaatc tgatgattgc atacctgcta
    10081 aatgttgtat tgctcctgat ataccacaag ctatatatag gtttggttta acagtagttc
    10141 ctgtttgacc tacttggtga gaatggtcta tccatccagc atcaacagcc gcacgagaag
    10201 aaccaactac tccacctaat ttgtcagcta atttcttaag catttcaaat ccttctggtc
    10261 cacctaatcc taaaccacct gatactataa catttgcatc tgtaagagat actaaatctt
    10321 tcttagtttt aactgtttct aaaacagtag ttcttatatc atcttgagtt attttgtagt
    10381 ctaatgctat aacttcacca gttcttgttt cgtctttttc agctttatcc ataacaccag
    10441 gtctaactgt agacatttga ggtctatggt ttggacaaat gattgtagcc attatgtttc
    10501 caccaaatgc tggacgagtt tgttttattt tcttatcttc tgggtctatt tctaacttag
    10561 tacagtcagc tgttaatcca gttccaactc ttgaagctat tctaggtgct aagtctctac
    10621 ctatatgagt tgctccgaaa agcattattt ctggttttat ttcatctatt gcatctttta
    10681 ttacttttgt atatgcatca gttgtatatt tttctaaaag agcatcatct gcaacataaa
    10741 ctttgtcagc tccaaaagtg attaattctt tagctaatcc atcaacatct tttcctaata
    10801 atattgcaca aagttctgca tctacttctt tagctaattt tcttccttct cctaataatt
    10861 ctataactac tggagctatt tttccttctc tttgttctgc aaatatccat acgtttttat
    10921 aagaacttaa atcttttata tcattcatag tttgtactcc tccaacttat ataatgtgct
    10981 tttgttttag accgattatt attttactag ctatttcatc agctttacct tctaagattt
    11041 ctccttttcc tcttggttca ggagtaaatg atctaaatac tttagttggt gatgctttta
    11101 aaccaacttc ttctttattt acatctaaat catcaattgt ccaaactttt acatcatgtt
    11161 tatatgcttt tactatttta tcaacagaca tgtatctagg tgtatttaac tcttttactg
    11221 ctgttaaaag tacaggtgta cttactttta ttaattcgta tccatcttct aattgtcttt
    11281 gaactattat atcttttcct tctattttga aatcttgtac atatgtaact tgaggaatgt
    11341 ctaatttctc tgcaatttgt ggtccaactt gtgcagtatc tccatctatt gcttgtcttc
    11401 ctgcaaatat tatgtcatag tcacctactt tagatattcc cgctgcaata gtatttgatg
    11461 tagcccaagt atcagctcct ccaaatgctc tatcacttaa taatattgca tcatcagcac
    11521 ccatagctaa acactctctt agcataaagt ctgcttgagg aggtcccatt gttataactg
    11581 ttatagttac atcatcatgc tcatctttaa tttttaaagc ttcctctaat gcatttgcat
    11641 catctgggtt taatatactt ggaactccat ctcttataag agtacctgtt tctttattta
    11701 ttcttacttc gttagtatct ggaacttgtt ttacgcaaac taatattttc aaagtctata
    11761 cctccaattt cattagatta tttattattt taatacgttt gatgaaatta ccattttttg
    11821 aacttctgat gttccttcgt atattgatac gattctagca tctctgtaca ttctttcgat
    11881 ttcataatct tttataaatc catatccacc gtgaagttgt aatgctttat aagcaacttc
    11941 atttgcaatt tctgatgcat agtatttagc catagcagat tcttgagcaa ctctcattcc
    12001 tgcatctctc ttttgtgctg catcataaac taatcctcta gctgcacaaa cttttgtttc
    12061 catgtcagct atagtaaatt gagtattttg gaatttagct ataggcttac caaattgaac
    12121 tctttgtttt gtatatttaa cagcttcatc taaagcacct tgagctattc ctagagctaa
    12181 ggcagcaaca cctattctac caacttctag agtacccatt gctatcttaa atccttgacc
    12241 ttcttttcca agtaagtttt cttttggaac tttaacgttt tcaaatatta agtcagaagt
    12301 ttcagttcct cttataccca tcttatcttc atgagctcct gttgagaaac catcccattt
    12361 actttctact ataaacgctg atgttcctct aagtccttta cttctatctg taactgctat
    12421 tactatagct acatcacaga atggtccatt agttatgaaa gtttttctac catttaatat
    12481 atagtggtct ccaactaatt ctgctgttgt ttgttgtcca cctgcatcag aaccagctcc
    12541 tggctcagtt aaaccaaatg ctcctattaa ttcacctgtt gctatacctt ttaagtattt
    12601 ttgtttttgc tcttcagtac cattgtaaag tattggtaat gaagcaagag aagaaccagc
    12661 tgtaacaaaa gtagctgttg aagcacatct tctagctatt tcttccatta ctatagcgta
    12721 agaaacatat cctgctcccc caccaccata ttcttctggg atattactgc ttataaattt
    12781 tgcatcagct aattttttga ttaattcctt aggcattaca cctgttttat ccatttctgc
    12841 tggtaaagta tctaattctt tactaacaaa atctcttact gcttttctta aaagttcttg
    12901 ttctttgtta tataacatag cttcctccaa ttttcatatt ttatagtaaa tttattttaa
    12961 tgaacttacg ctaaactcat catctcagca aaagtttgaa tacgagttct ggcttgttca
    13021 ttattctgag tttgttggtc gatttcaaca tataaagtag gtattccact atcttctata
    13081 tcttttctaa ctaaaggata atcgtattct tctgggtcac agaatttcat catacagaag
    13141 ataacaccat ctatatcttt ctttttaact tcatctacta taagtgaccc acgttttttc
    13201 ttagggtcat aagctaatga acatccttct atgtttgacc attgtcttgc taatctctct
    13261 aacgcatcat cacctgctgg tacatctgtt ctgaattgtc ttgtttcttg tgctaagtcg
    13321 tcagctacaa ctgatatatt gttgtcttct aaaatgtcta atatatcttt agaatcagct
    13381 aatatacctg ttaataaaac tttctttcca gaacagactt cttctggcat agcatttaat
    13441 tttgctatta aatctttcac tagctcagta tgttcttctt ttctcataaa gaacccactc
    13501 ttaattacta agcttcttat tgatggtttt atagtattag aatgtttata agctacttca
    13561 acaaagtctc tcatagtttt tctatgttca ttgtaaactt ctatactttc atgaattttt
    13621 gcttcttcta tttcatatcc acaaatttct tcaagttctc tttttacacc tttgtactca
    13681 ctgattaagt attttacacc agcttcaagt tttctatttt gtgggtgtac taatgatata
    13741 tattttatat gaggtactgc tgatttccag ttttgtccta aacaaattag tgtatcacac
    13801 atacctggta taataactcc agataattca tcataagcac cttttaatcc atattctaaa
    13861 catgattgca ttattgaaca tgcaaatgca gggaaatatt gtttagctaa atctaattct
    13921 gtttgtcctc cccatatacc aactggaagc attccagctg catgtataat ttcttctggg
    13981 caataaactg ggaaacaacc tatagctttt ttaccagttt cacttttata tttttttaca
    14041 gccgcatttg gattttcaac tacttctttc attttagata aaatagcttc cattatatct
    14101 cacctctatt aagttttttt ctttcttcca ttacttcaac taaaccttga attcttgttt
    14161 caaattgagc attagtgaaa gctctagggt ctgcttggtc accatcaaat ccagcatatg
    14221 gtaatccagt ttcttcagct gctcttcttt gcatttcata ttgtattaaa ctcataagtt
    14281 tacagcttct gttcatatga tagaatgctc catcacattt accctctact aaagaatcaa
    14341 ctctatattt tgtcatacgg tctaggttta cattgttaaa catagtacta tatgctactg
    14401 ccattccatc taaatcatta acttcatatt gtaatgccca agcatgtggg taaacactac
    14461 ctgtcatgtt aactccaaat ttagctaatg ttttcatctt gtatcctata tatggccaac
    14521 aaggtatacc ttccatcatt attctgtatt tttcttcccc tctgaaagaa gatttaccag
    14581 ttttcatgtt gtcctctaat tcttctataa gtaacttaaa tgcttctgta gtttcttttt
    14641 tacctctagc acaaactatt acagccatgt aagtaaataa gtcaaatcca ttcattggag
    14701 aaggagaaga atctgctggt aaactcatag aatacttcca tagtcttccg ttttcagctg
    14761 atattttcat tacttcttca aacttcttag ggtcaaattt ctttcctgat ataatttcta
    14821 gttgttttat agcttcttca aattgagctt taatataatc tattcttgat tgagtaactt
    14881 cgtcttcatt attgaaagtt gtatcaatca ttattaaagg tatatctaat tctcttgaaa
    14941 tattttcata ccattttata acttggttac atatattgtt acagcaaagt aggaaatctg
    15001 gagccggcat atccaaagct tcacatccac cattttctaa aagaccaaaa tttgttcttg
    15061 catatgcaca taggtcaata gaatatcctt tagattcagc tatttcacat aattctaaag
    15121 aacctttttt agctgctact ccagctgctt ggttttctgg atataatacg tttaagtcaa
    15181 atacttctgc taactcttga ggaaatactg atgttgacca acctacaggt cttccttctt
    15241 ctttagcttt aaatgcattt gcatattgtt cagctaataa atcattaatt actactctag
    15301 cttctttttt ttcagacatt ttcatccctc tttttatata tttttcactt ctttttgtga
    15361 ttcttttgct tcatcaaaag catataacgc tgctcctaaa gcaccagtta attgaggtat
    15421 atcaggtaca ataatttctg tgttgatttc tcttgccata gctcttacaa taccactatt
    15481 tctagcaact ccaccaacca taactacatt tctttgtact cctattcttt ttactaggct
    15541 agaaactctc tttgctactg aagtatgaat acctgccact atatcttcaa tttttgcatt
    15601 ttcagataaa tgtgatataa cctcagactc tgcaaatact gtacatgtac tgcttattga
    15661 tacttcattt tgagaattca tagatatact tccgagttca gatacatcaa cctctattat
    15721 ttttgccatt acatctaaaa atcttcctgt acctgcagca catttgtcat tcataagaaa
    15781 gtttaatagt cttccattat tatctaattt taataccttt gcatcttgac cacctatgtc
    15841 aataatggtt cttgtctctg gaattatgaa atttacccct ctagcatgac agcttaattc
    15901 acttatttgc ttatcagcat ctgaatagtt cattcttcca tatcctgtaa ctacaacttt
    15961 tttaatatct tctcttgcaa gacctgtctt gccatataat ttttctaaaa ctcttgatgg
    16021 tccagtagtc ccagtaccag aggatattgt ttcagaagct acaatatctt ccccattctt
    16081 taagattact ccctttgatg cagttgaacc tatatctaat cccattgtgt acatctttag
    16141 cattctcctt ttaaaaaatc tagaattact aattaatatc ttacaacttt actatcttta
    16201 aagttattta ttttctcttc atcgtagcct aaagatttta atacttctac agtatgttga
    16261 cctacttttg gtgctggtgt atattcttta attccttcat ttctaaacat aactggagta
    16321 ttaactaaga cacctgtatt tccgctatcg tatgttttct taaataagaa gtcatttgcc
    16381 caagcttgtt cgtcatctaa taaatcttca cagctttgaa ttttttcaaa tggtaagtct
    16441 gcttcttcta ataaagctga ccactcgtct aatgtttttt ctaacatagc ttctccaact
    16501 atcttaacta aatcttcaac atgattaacc attgaatcta tgttattata tctatcgtct
    16561 tctaatatat attctctatt tataacctta cagaatttgc ctaaccactt gttgtattgt
    16621 attaaagcta attgaatcca tcttccatct ttacatttat atgtagtcat taatgggctg
    16681 tttggatttt ctcttgataa aggcatttca tttccgtatt gtgctgttgt tatcattgtt
    16741 cccattccat atatagctgt atggaaaaga cttactgtta ctctctcacc tttaccagtt
    16801 tgagcttttt tatgtaatgc tgctaaactt cctgctgcta gtgctagacc tgcatagtgg
    16861 tcaccaaatc ctgctgctgt atttgctgga gatgttcctt tttccataac agattggcta
    16921 actcctcctc ttgcgaagta tgcagtatag tcaaatcctg gtttatcttt taaaggtcct
    16981 ttttcaccat atcctaatat ttgagagaat attaatcctg gatacttatc ttttatttgg
    17041 tcataagcta tacccatttt ttctaatgct tgaactctaa cattagttac aaatatgtct
    17101 gcttctgata ataatttatg taatatttct actccttctt ttgattttac attaatactt
    17161 acaccctttt tatttccatt ttctaattca aacatagggt tttcatcatc tgatgctgga
    17221 gatttaaatg ttccacccat aactcttatt ccatcacctt ctataggttc aatcttaata
    17281 acctctgcac cccagtcacc taacattttt gcacaacatg gtgctgcgat gaaacttgaa
    17341 agttctacta ctttaactcc ttctaaaagc ataacgtttt cctccttaat agtatcaatt
    17401 ataaaatttc gattaaatca ctcataaaat acttttttct gatattaact gtctgatgtc
    17461 aaagttccta atcttatcta aaataactta aaaattatta aaattatttt ttattgtttt
    17521 attgtcttac acctatattg ttaagcattt atcgtgccaa ctttatattt aatcaaaata
    17581 tcgcttgatt tgaatgtttt ttaacaatat gcaattgaat aatctttttc atatcgtggc
    17641 atcttgccac tatccattgc attttgctat tctttctatg cttataaaag ttttttttat
    17701 atattttttt atgaaaaagc ttgacatttc aaaaaaagtt ttcattgcta aactcaaaaa
    17761 cacatgttat cattgtaatt aatcagcaag ttagttaact aatttcaaac ttagataatt
    17821 ttttaataat atttaatcta aaaaataaca atttctttga taaattttag agtaattatt
    17881 acattaaata aaaaattact aaatatcaaa ataacacagg gaaaacaatt tcaaaactaa
    17941 atttttaacc ttaaaaattt tgtataaaaa atataacctt atatgctaca tattatttct
    18001 aaatgaaaaa ttaccataat aatttaataa ttacaggagg gtattgatat gaaaatacta
    18061 gtatttggag cacgcgatta tgaagaacca gtaataaaaa aatggtctga agaacataag
    18121 gatgttcaag tggatattta tcctgaaaac atgactgaag aaaatgtagt taaagctaaa
    18181 gggtatgatg gtatatctat acaacaaact aactatatag ataatcctta tatttatgaa
    18241 actttaaaag atgctggggt taaagttata gcttcaagaa ctgcaggggt tgacatgata
    18301 cattttgatt tagttaatga aaatggactt atcgttacaa acgttccttc ttattcacct
    18361 aatgcaatag ctgaattagc tgttactcaa gctatgaacc ttttaagaaa gactcctcta
    18421 gtaaagaaaa aagtctgtga aggtgattac cgttggatag ctgaacttct tggaacagaa
    18481 gttagatcta ttacagttgg tgttataggt acaggaaaaa taggtgctac ttctgcaaaa
    18541 ttattcaaag gcctaggagc taatgtaatt gcatttgacc aatatccaaa tagtgattta
    18601 aatgatatat taacttacaa agattcttta gaagaccttc taaaagaagc tgaccttata
    18661 acattacata ctcctttact tgaaggaaca aaacatatga taaataaaga tactctagct
    18721 ataatgaagg atggagctta catagtaaat actggccgtg gtggtttaat taatacaggg
    18781 gatttaatag aagcactaga gtcaggaaaa attagagctg ctgcccttga tacatttgaa
    18841 actgaaggat tgttcttaaa caaaaaaatg aatcctggag aattaactga cccagaaata
    18901 aataaacttc tttctatgga acaagttata ttcactcatc accttggttt cttcactagt
    18961 acagcgattg aaaatatagt ttattctagt ttaagtagtg ctgtagaagt tataaaaaca
    19021 ggaactgcta ctaatagagt aaattagtat tttataaata tattttttca ttaattagct
    19081 attatctaac aaaatagcct ttaatttccc aaatataaga gagtcagctc agtatatatt
    19141 gagtagactc tcttatatta ttttttatat taaacttgta ctcttcttat tcataccaaa
    19201 ttaaatttat attaacttac tctaactact cctactctat acattttacc tatttttact
    19261 tttctaatta tatacatttg aatgggtaca atctgtattt tacaaaaaaa acacctaaca
    19321 aactatgaaa attgtatgat taatattaat atatttataa gaaaacataa caattatgtt
    19381 agctctggat ttttagggag ggagtatatt ggaaaaaatg aataaagtta cttctaaaaa
    19441 aactacctat attgttactt cagcattatt tgcatccata atatgcctga caatagcata
    19501 tatattacat attccagttg gtggaaacaa tggatatgtt catataggag atgcttttat
    19561 ttatctagct gcaactattt tgccaacaaa ctatgctata gctgcttctg caattggtgc
    19621 tggtctagct gatttatcta caggtgctgc tatctgggta ataccaacta taatcataaa
    19681 accaatactt gtattatttt tcacttctaa aagtgacaag ataataaata agagaaatat
    19741 agttgcttcg gttgtagcag gtatagtagg attagtttta tacatgtttg ctgaaggtat
    19801 aattattgga agttttacaa gtgcatttgt aatgagctta cttggcttat tacagccaat
    19861 aggaagtttt attgttttta taatcttagg tatggcactt gataaacttg attttaaaaa
    19921 acgttatttt aattaagaat ttaaatttat ggagggaaaa ttatgaatat cttatataca
    19981 attgaaaata gtatctatgt aaatataact aatacatgtc catgtagttg tgttttttgt
    20041 ataagaaacg aaaaagatga agttgcaaat agtggtagtt tatggttgga gcatgagcca
    20101 agtgtagatg aagtaaaaga agccttcaat aaatacaatt tagacgacta tgatgaaatc
    20161 gtattttgtg gatatggcga accattaatg agaataaatg aattgataga ggtagcaaag
    20221 tttataaaag aaaaaagtag cataaagata agaataaata caaatggtct aagtgattta
    20281 atacataaca aaaaaacagc tattctgtta aaaaatgtta tagatgcagt atcaataagt
    20341 cttaatgccc caaataaaga agcttataat agagttactc aaccaaagtt tggagaaaaa
    20401 tcatttgatt atatgttaga ttttgcaaga gattgcaaaa aatatataaa agaagtagct
    20461 ttttcagtag tagatgaaat atctccagaa gaaatagaag aatctaaaca actggcaaaa
    20521 aaacttgata taccattaag ggttagacat aagaattagc aagtaaaatt ataaaacatt
    20581 aagaatcacc tgaatggtct ttcaggtgat tcttcctctt gtattaacat tttacaaaat
    20641 agatttcata ccacactagg aatgtgtaaa tcatccaaat ctttctccaa ttatcagagt
    20701 ttccactaat gtagtcatta aaaatctcat taatctcatc tatgttaaag aatttatctg
    20761 ctatctcact attgaattta tctcggatat ccttattata ttggtcatca gccatccata
    20821 tacgaattgg cacaataaat cctaatttat ttctaaaagc aatctcttct ggtaatgttt
    20881 ttgatgcagc tgtacgaaag gcaattttat tctcttttcc tttcacttta tattctgatg
    20941 gtattctgga tgcaatatca aatattcttg tatctgtaag aggcattctg acttccaatc
    21001 ctactgcagt actcatttta tctatattaa aataaatatc tccttccaac catatttgaa
    21061 tatctacatt tgacatgtta gtaagtgtat ctagttcttc tgtttcttca taaattcctt
    21121 ttaccacttc aataggaagc acatcaggat tgtatttctt aagaattttt cttttctcac
    21181 cctcattcat aatgtttgta tttccaatat aaaatgtctt caaattctct ccataacact
    21241 ctgccttatg gtatatatta tatccaccaa aaaactcatc tgctccttct ccagagtaac
    21301 agatatttgt gttttcagct gctgccttgc atccaatagc aaaaacgata gcagatgcat
    21361 ctccagttgg ttgctccata tggtacatta cataaggtac tgacgcaaaa tactcttcag
    21421 gtgtaattat atatctcgaa tttttacacc caagaatatt agctgtctgt tgtgctaatt
    21481 ttgattcatc aaaacgctct tcctcataac cacatgaacc tgttttttct atatctgaca
    21541 ttgccaatat ataagatgaa tcaacacctc ctgataggaa taactctgca gtttcattct
    21601 ctgattttac ctctggcata attttctgaa gtgttgtatg aatttcatcc gtccattcct
    21661 caatagactt gctgtggtca ggatgaaatt caggtttcca ataacgacca atacatactg
    21721 ttttattctg atagacaagg taatgtcctg gcataagctt ctttactcca cggaagaatg
    21781 tattttcacc tgctacatat gtgaaagaca tataaatctg taacatgtct tcatttaact
    21841 ctttcacaaa tcctggttgc tccataatct tacgaataga tgaaccatag agaagcttcc
    21901 cattttcagt ctcataataa tagaaaggtt ttaccccaaa ctggtctctt aggcagaaga
    21961 gtttcttttc cacagtatcc cataatgcaa aagcaaacat tccatgcatg tgatttgcca
    22021 tatcatatcc ccatgtctta tatgcttcca ctaaaattgc tcgttcacgc tcatctctac
    22081 ataatgaaga atcgattcca agttcttcac aaagaacttt ccagtttcga atatgcccat
    22141 tgtattctag aatttttatc atatatgtgt cttttcgtgt taaatacttt tcttctaaat
    22201 ttatcatatg tgtatccttt cgtattaaaa catttaactt ctaaatttat taaatgttta
    22261 tatatattta ttcacttata atcactgaaa aatcgacttt tttcaaagta aattcttttt
    22321 gaattagtac taatctgctc tttattaatg gcataaatat gattatcaag tattatcagt
    22381 gttataacat tgaatttttt attcttcata aatattgccc cctttatatt accaactaa
//
LOCUS       DAMJWU010000054        22363 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMJWU010000054 DAMJWU010000000
VERSION     DAMJWU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.054"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            100..1551
                     /locus_tag="PC460_002262"
     CDS             100..1551
                     /locus_tag="PC460_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2601145.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1749..2390)
                     /locus_tag="PC460_002263"
     CDS             complement(1749..2390)
                     /locus_tag="PC460_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2601146.1"
                     /translation="MTDNYIFDSYNNLVDGLSAYLGEGYEFILYKFNGVHYSINKIAN
                     GKYNTVKEGDIITDFDLALINKLENDADGDFISYFNRNKLGEPLKSTDIVIRGVDNKI
                     IGLLCIHFYLNTPLTRIIGSYIPEHNAMQSAPVLENIVEDTDEVILKSYQDAKVIVEN
                     DSNISSNDKNKSIVSILNESGIFKIKDSVVKVADLMGLSKNTIYMHLRNLNKN"
     gene            2864..4243
                     /locus_tag="PC460_002264"
     CDS             2864..4243
                     /locus_tag="PC460_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2601147.1"
                     /translation="MAQDKIVVEILENIGGKENIKEVAHCMTRLRIKVCDSSQVNQEK
                     LKKIKGVLGLVLKGEQYQIVIGPNVSQVYNTLNEVIGIIPEKDSKKTISEGKQNSIAK
                     ILDTIAGIFNPIVPALAGAGLIKAILALLVASKFLSADSETYIILNTISDGVFSFLPF
                     MLAYSAAKIFKMNTYVAVAIAGAMLHPNFINLIQQGLTKISFIGIPMSLIQYNGSVFP
                     IVLTIWFASYIEKGVDKITPKALKIILVPALTILFAAPIALIIIGPIAQMLGNYIAGG
                     ASILFERGGIFAGLIYGGIYSFMVVTGLHHGMVPVLVDGITRYGFNYISPVSGSSNMA
                     QAGSAFGVWLKAKDKDLKAVAATATISALTGVTEPAIYGVNLRLKKPFLCAAIGGAAG
                     GGFAAYFQCKAFAMGGPSFLTLPMFIGEGGHVGLVAIAFTIAFIVSAIATYIVGFEEE
                     VTADSHANL"
     gene            4287..5021
                     /locus_tag="PC460_002265"
     CDS             4287..5021
                     /locus_tag="PC460_002265"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010990049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2601148.1"
                     /translation="MKIYLTKDYEEMSQVAAQMVLKYMYSNDNKRINLSITAGQTPKR
                     TYEILSEFVKNKPYFENVHYYNFDEIPIKGEKVGVTIRNLTNMYFREAQIPEERIHVL
                     DENNYQGFDRKIEADGGIDMVLMGLGWDGHFCGNLSGTVNSFDEETRAVSTYITDDMA
                     KLLTKSVGGNPEKLFDYYVTYGPKTIMHCKNIIMIVNGKHKAQILKQTLFGPLDLLCP
                     SSLLRLHPNFTLIVDEEAASEIKDLI"
     gene            5110..6309
                     /locus_tag="PC460_002266"
     CDS             5110..6309
                     /locus_tag="PC460_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2601149.1"
                     /translation="MYNHMKKVFQGLEGGLFSSVEKADVGDSYQKLGEQGVSLMGWAD
                     PFFPDYCIPRHILDAATLAISSNMSTHYTAPVGNDLLKIEIAEKLKEVNNLDVDFKRN
                     ILITPGSDSGLYYSMLPFINEGDEVLIPTPCYPNNIQNTKLMGGVPIFVELKESDGYQ
                     IDITEFEKRLTNKTKLVVLTHPNNPTTTIFNKESLEKLAKFIIDNDLILICDQAFEDF
                     TFENELITPASLEGMFERTVTIFSTSKGMGLSGFRVAYMVCSDKLMDKYYGAAVTVLG
                     ATNTVAQMSTIEAFKDRSFMEDFERVFDKRRQEAYKIINEIPNVSMQMPESGFLGWIN
                     VSKLGTSSEIQKYLVDEAKVCINDGINYGPGGEGHLRIILGSYKDDSKVIGALNRIKD
                     ALIKKQQ"
     gene            6333..6815
                     /locus_tag="PC460_002267"
     CDS             6333..6815
                     /locus_tag="PC460_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2601150.1"
                     /translation="MLSIFKSKKNDNKLKAFASGKVIPIEDVDDNVFSSKMLGDGIAI
                     YPTNEYVVAPCDAEVNIIMKDTKHAVGIKLFNGMEILFHVGIDTVSMNGEGFEIFVSP
                     GDKIKRGDKLISFNSKLIKEEGLQDVVIMVVTNSEQYKNIKYHIGLEAVIDDTIIAEI
                     "
     gene            complement(7133..7730)
                     /locus_tag="PC460_002268"
                     /pseudo
     CDS             complement(7133..7730)
                     /locus_tag="PC460_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7974..8402)
                     /locus_tag="PC460_002269"
     CDS             complement(7974..8402)
                     /locus_tag="PC460_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601151.1"
                     /translation="MKIHSIQNLTLDTRNLIKKYSLTLNEDLIWEFKHGKYHTIKYFT
                     HKFAVKESVLALLFNIHKLCYAKIKYFESSFSKYEPYKYSYQNEFVKCEMYDMNFMFH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQDLII"
     gene            complement(8803..9264)
                     /locus_tag="PC460_002270"
     CDS             complement(8803..9264)
                     /locus_tag="PC460_002270"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF2601152.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
     gene            complement(9369..10562)
                     /locus_tag="PC460_002271"
     CDS             complement(9369..10562)
                     /locus_tag="PC460_002271"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF2601153.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(10585..11250)
                     /locus_tag="PC460_002272"
     CDS             complement(10585..11250)
                     /locus_tag="PC460_002272"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF2601154.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(11284..12396)
                     /gene="ribD"
                     /locus_tag="PC460_002273"
     CDS             complement(11284..12396)
                     /gene="ribD"
                     /locus_tag="PC460_002273"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF2601155.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(12528..12649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12838..15320
                     /gene="recQ"
                     /locus_tag="PC460_002274"
                     /pseudo
     CDS             12838..15320
                     /gene="recQ"
                     /locus_tag="PC460_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     regulatory      15409..15520
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15662..16963
                     /gene="thiC"
                     /locus_tag="PC460_002275"
     CDS             15662..16963
                     /gene="thiC"
                     /locus_tag="PC460_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2601156.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            17017..17211
                     /gene="thiS"
                     /locus_tag="PC460_002276"
     CDS             17017..17211
                     /gene="thiS"
                     /locus_tag="PC460_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF2601157.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            17204..18007
                     /gene="thiF"
                     /locus_tag="PC460_002277"
     CDS             17204..18007
                     /gene="thiF"
                     /locus_tag="PC460_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF2601158.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            18070..18840
                     /locus_tag="PC460_002278"
     CDS             18070..18840
                     /locus_tag="PC460_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF2601159.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            18857..19960
                     /gene="thiH"
                     /locus_tag="PC460_002279"
     CDS             18857..19960
                     /gene="thiH"
                     /locus_tag="PC460_002279"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF2601160.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            19961..20650
                     /locus_tag="PC460_002280"
     CDS             19961..20650
                     /locus_tag="PC460_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2601161.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            20838..22232
                     /gene="yfcC"
                     /locus_tag="PC460_002281"
     CDS             20838..22232
                     /gene="yfcC"
                     /locus_tag="PC460_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF2601162.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         8329 a         2655 c         3715 g         7664 t
ORIGIN      
        1 ttataattaa attaatgaat ttttaacaag gaattattca tttttttgat tttaagattt
       61 attgagaaaa caaaactaaa taggggggta attaatataa tggatttttt caatggtatt
      121 ttaacgagta ttaacaatgc agtagtattt gtaaacaatt acttatggtc gtatatctta
      181 attataatgt tagttgtaat tggtatttac tttactatta aaacaaattt tgtacaattc
      241 agatatttta tagaaatgtt tagacttctg ggtgatggta cagcgaataa aaatgcaaaa
      301 aaggaaggaa aaatctcttc atttcaagca ttttgtataa gtacatcttc aagagttgga
      361 actggaaata tagctggaat agcaattgca gtggtagcag gaggacctgg tgctgtattt
      421 tggatgtggc ttatagcatt aattggttct gcatcaagct ttgttgagag tacattagct
      481 cagatttaca aagtgaaaga tggtcaagct tttagaggtg gaccagcgta ttatatggaa
      541 caaggtctaa ataaaaaatg gatgggtatt ttattctcaa tacttataac tatatgttat
      601 ggttttgtat ttaatgcagt tcaagcaaat actgtttcac ttgcatttaa taatgcattt
      661 ggaataagta aaatggctat gggaataggt cttggaattt taactgcaat agttattttt
      721 ggtggagtgc atagagttgc aaaagtttct gaaataatag ttcctgtatt tgcaggatta
      781 tatatattag ttgctttggc tatagttgta atgaatataa cagagatacc aagtgttata
      841 gcattgatag ttgaaagtgc atttgatttt aaaggaatgg cgattggtac atttatggga
      901 gttgtaatga ctggagtgaa gagaggtcta ttttctaatg aagcaggtat gggtagtgct
      961 cctaatgctg ctgcaacagc acatgtaact catcctgtaa aacaaggttt aattcaatct
     1021 ttaggtgttt ttacagatac tataataata tgtagttgta cagcattcat ggtattatta
     1081 tattcaggtt atagtagtag tggagcaact ggaattgaat tagctcaaga agctttaact
     1141 catcatatag gaccagttgg aaatatattt atagcagttt gtatattctt gtttgcattt
     1201 agttctatag ttggtaatta ctattatggt gaatcaaata tggagtttat gagtggaagt
     1261 aagactaaac ttaatgtatt tagagttttt gttgtaggta tggttttatt tggttcactt
     1321 actcaagtgg atgtagtttg gaatcttgca gacttattta tggcattgat ggcgataata
     1381 aacttaatag caatagcact tttaggaaaa tatgcattta ttgctttaaa agattataca
     1441 agtcaaaaaa aatcaggaat aaaagaccct gtgtttattg cagatgaaat agaaggccta
     1501 gaaaaggtat cagagtggca tagggaatta tctgaggaac atttaggata aaaaatagta
     1561 taaaattata aaaccatttt gaaagactag agtatgtata tactttatat gaatataata
     1621 ttgaattaat ttatattact ggaattttta attccagtaa tattttttta attacaagga
     1681 tattatgatg acttaaagac tgtggaaaac tcttttttag ttttcattat taatattaat
     1741 tgacaaattt agtttttatt taaatttctt aagtgcatat aaatcgtatt ttttgataat
     1801 cccattaagt ctgcaacttt aacaacagaa tccttaattt taaaaatacc actttcattt
     1861 aaaatagaaa caatagattt atttttatca ttgctagata tattagaatc attttcaaca
     1921 attacctttg catcttggta tgattttaaa attacttcat cagtatcttc tacgatattt
     1981 tctaaaacag gagcactttg catagcatta tgttccggaa tatatgaacc aattattctt
     2041 gttagtggtg tattaagata aaaatgtatg cagagtagac cgattatttt attatcaact
     2101 ccccttatca ctatatctgt tgattttaaa ggttcaccta gtttgtttct attaaaataa
     2161 ctgataaaat ctccatcagc atcattttcg agtttattaa tgagtgccaa atcaaaatca
     2221 gttataatat ctccttcttt tacagtatta tatttaccat tagctatttt atttattgaa
     2281 tagtgtactc cattaaattt atataaaata aactcgtatc cttctcctaa atacgcagat
     2341 aatccatcca ctagattgtt ataagaatcg aaaatatagt tatcagtcat gttaaaagtc
     2401 atatgttacc tcctaagaaa tatcttaata tcatattaac atataaaatg aatttaataa
     2461 aatatatttc agtgaaataa taaatataat gaaaaaaaaa ctattgactg aaaaatattt
     2521 cagtgatatt attaaaatac agtaaggcaa acgtattatt gacagaatat ataaaatgtt
     2581 aaaaatagta tctaggaatt aaacaaatta aaggactagt gatttgaatt tttttagtat
     2641 aatatgtgac ttccaattaa tatttattaa acaccaataa aattgtaaat atgcttaatg
     2701 atatacttta gcgatgaagt gaaaatattt tatttttatg attaacctat aggaattaat
     2761 ttagcatact gtttagctag taaatctaaa taaattttag ctttagctga tatcatgttg
     2821 aataaagttt ctattggatt ttgtataaca aaggagtggg gagatggcac aagataaaat
     2881 tgtggttgaa attttagaaa acattggtgg taaggaaaat attaaggaag tagcacattg
     2941 tatgactaga ctgagaataa aggtatgtga tagttctcag gtaaatcagg aaaaactaaa
     3001 gaaaataaaa ggtgtactag gtcttgtatt aaaaggtgag caatatcaaa ttgttatagg
     3061 tcctaatgta tcacaagtgt ataatactct taatgaagtt ataggaatta tacctgagaa
     3121 ggatagtaaa aaaacaatat ctgaaggtaa acaaaatagt attgcaaaaa tattagatac
     3181 tattgcaggt atttttaatc ctatagttcc agcattagcg ggtgcaggtc taataaaagc
     3241 tatattagca ttactagtag catcaaagtt tttatctgca gatagtgaaa cctatataat
     3301 attaaataca atatctgatg gtgtattttc atttttacca tttatgttag cttactcagc
     3361 agctaaaata tttaaaatga atacttatgt tgctgtagca atagcaggag caatgctaca
     3421 tcctaatttc attaatttaa ttcaacaagg cttaactaaa atatctttta tcggtattcc
     3481 gatgtcacta atccagtata acggaagtgt atttccgata gtactaacta tttggtttgc
     3541 atcgtatata gaaaaaggag tagataaaat aacaccaaag gcacttaaga taatattagt
     3601 acctgcattg actattttat ttgcagctcc tatagcatta attattattg gaccaatagc
     3661 tcaaatgcta ggaaactata tagccggtgg agcaagtatc ctatttgaaa gaggaggaat
     3721 atttgcaggg ttaatttacg gaggaattta ttcattcatg gtagtaacag gattacatca
     3781 tggtatggtt cctgtattag tagatggaat aacaagatat ggatttaact atataagtcc
     3841 tgtgtcaggt agttcaaata tggctcaagc aggatctgca tttggagtat ggttaaaagc
     3901 aaaagataaa gatttgaagg ctgtagcagc tacagctacc atatcagcat taactggagt
     3961 taccgaacct gcaatatatg gtgttaattt aagattaaag aaaccgtttt tatgcgctgc
     4021 tataggtggt gcagcaggag gtggatttgc tgcatatttc caatgtaaag catttgctat
     4081 gggaggtcca tcgtttttaa ctcttcctat gtttatagga gaaggtggac atgtaggatt
     4141 ggtagcaata gcatttacaa tagcatttat agtatctgca atagctacct atatagtagg
     4201 atttgaagaa gaggtaacag cagattcaca cgctaactta taataaagag aaattattta
     4261 acatatcaaa aaggagatta attactatga aaatttattt aactaaagat tatgaagaaa
     4321 tgagccaagt ggcagcacaa atggtgttaa agtatatgta cagcaatgat aataaaagaa
     4381 taaacctttc tataacagca ggtcaaacac caaaaagaac atacgaaata ttaagtgaat
     4441 ttgttaagaa taaaccttat tttgaaaatg tacattatta taactttgat gaaataccaa
     4501 taaaaggcga aaaagttgga gttactataa gaaatttaac taatatgtac tttagagaag
     4561 ctcaaatacc agaagaaaga atacatgtgt tagatgaaaa taattatcaa ggctttgata
     4621 gaaagataga agccgatggt ggaatagaca tggtattaat gggattaggt tgggatggac
     4681 acttctgtgg aaatcttagt ggtacagtca actcttttga tgaagagaca agagctgttt
     4741 caacttatat aacagatgat atggctaaac tattaacaaa atctgtaggt ggaaatccag
     4801 aaaaattatt tgattactat gtgacatatg gacctaaaac aataatgcat tgtaaaaata
     4861 taataatgat agtaaacgga aaacacaaag ctcaaatatt aaagcaaaca ctatttggac
     4921 cattagattt attatgtcca tcatctttat tgagattaca tcctaacttc acattaatag
     4981 tagatgaaga agcagcatct gaaataaaag atttaatata aataagtaag gaattaaaat
     5041 taaaaatgaa tttaggtgca aagtaatatt ttattttgca cctttgcttt aataaaaagg
     5101 agaataaaaa tgtataatca tatgaaaaaa gtattccaag gattagaggg tgggttattt
     5161 tcaagtgtag agaaagctga tgtaggcgat tcttatcaaa aattaggtga gcagggtgta
     5221 agtttaatgg gatgggcaga tccatttttc ccagattact gtatacctag acatatatta
     5281 gatgcagcta cattagcgat atcaagtaat atgtcaactc attatacagc gcctgtaggt
     5341 aatgacttat taaaaattga gatagcagaa aaattaaaag aagtaaataa tttagatgtt
     5401 gattttaaaa gaaatatatt aataactcct gggtcagact cagggcttta ttactcaatg
     5461 ttaccgttta taaatgaagg ggatgaggtt cttataccaa caccatgcta tccaaataat
     5521 atacaaaata ctaaattaat gggaggagtt cctatatttg tagagttaaa ggaatcagat
     5581 ggatatcaaa tagatataac agaatttgag aagagattaa ctaataagac gaagttagta
     5641 gttcttactc atcctaataa tccaacaact acaatattta ataaagaatc tttagaaaaa
     5701 ttagctaaat ttattataga caatgattta atattaatat gtgatcaagc atttgaagat
     5761 tttacttttg aaaatgaatt aataacacca gcttcattag aaggtatgtt tgaaagaaca
     5821 gtaacaatat tttcaacatc aaaaggtatg ggattaagtg gatttagagt tgcatatatg
     5881 gtatgcagcg ataaattaat ggacaaatac tatggagcag cagtgacagt attaggtgct
     5941 actaatacag tagctcaaat gtcaacgatt gaagcattta aagatagaag ctttatggag
     6001 gattttgaaa gagtttttga taaaagaagg caagaagcat ataagataat caatgaaata
     6061 cctaatgtat ctatgcagat gcctgagtcc ggatttttag gttggataaa tgtatcaaag
     6121 cttggaacat catcagaaat acaaaagtat ctagttgacg aggctaaagt ttgtattaat
     6181 gatggaatta attatggtcc aggaggagaa gggcatttga gaataatttt aggaagctat
     6241 aaagacgata gtaaggtaat tggtgcatta aatagaataa aagatgcttt aattaaaaag
     6301 caacaataaa taaaaaataa ttggagtgga gtatgttatc aatatttaag agtaaaaaaa
     6361 atgacaacaa gcttaaggca tttgcatcag ggaaagtaat tcctatagaa gatgttgatg
     6421 ataatgtttt ttcatctaaa atgttaggtg atggaatcgc tatttatcca actaatgaat
     6481 atgttgtagc cccttgtgat gcagaagtaa atataataat gaaagatact aaacatgcag
     6541 ttggaattaa attatttaat ggtatggaaa tcttatttca tgttggaata gatactgtat
     6601 ctatgaatgg agaaggtttt gagatatttg tttcaccagg tgacaagata aaaagaggtg
     6661 ataaattaat atcttttaat agtaaattaa taaaagaaga agggttacaa gatgtagtta
     6721 taatggtagt tactaattca gaacagtata aaaatataaa atatcatatt ggactggagg
     6781 cagtaatcga tgatactatt attgcagaaa tttaaaaaca gttatttaag taaatactta
     6841 gataatttag atatagttaa aattaaatcc ttaaaatcaa attatttaat ttttgtgtaa
     6901 ttttaactat aaagccgatt aataatataa agtatactaa ataatatggt tattatgatt
     6961 gttactgaca aagcctaagt agatataaca atttgctcta attgagtatt gctatatcta
     7021 cttgttttta tatttaaaat aaaagagtct gcattgaaaa gataattctg atgagagtaa
     7081 atagggtatt taataatagt ttaattctga cttatcaaga tgaacctatt catataccta
     7141 aaacatcttt aacaccttct aaattcatac aaatagctgt ataggctgcc ttattgatta
     7201 tttttccgtc aatttttact ttgtagtgaa cagcatatat aaatattatc gaatagattg
     7261 tatcaagagc tctattttgc tattcattag ctaaagatag tactctgtca gttatttttg
     7321 aaactaatgc atgagatata tcaattccgt aaatattatt taaatgtatg atatatatcg
     7381 cgagtcgaca ttccttttga gtacatgcca attacttggt tctctattcc agaaatttca
     7441 ttttcgtaat tttgaataac cctaggctgg aaatcatccc tagggatatt taattttacc
     7501 tctccaaaat ctgatttaac attcttagct ctatatccat ttcttgaatt tgatgttgct
     7561 ttactttggt tatcatatct ctcgtacatt aaatgatctt caatctctga ctctaacatt
     7621 ttgtgaattg tgcttgcgaa taaatctttt aacatctctt ggatgtcttt agtatttttt
     7681 acatcatact cttgaactaa gtgttttaat atttaatttt tgttgtacat aaaaaatgtg
     7741 tacctcctat tttggtaatt agatttaata tcttgacttt taggagaata cacattttat
     7801 tttacagacc ctcttttcta gaaaagtttt tatgaaaatt gtgatataaa ttattaataa
     7861 aaaataattt tatatttatt ttattatgta atttagtttt attaaaaatt ttttattatt
     7921 ggatatagat tatctattat aaattttatc tttaagttta ttctcctaaa atactatata
     7981 attaaatcct gtaaattatc ttcaaagctt tctaggtaat cacaaaataa tctaaattct
     8041 tctatggttt taatttcttg taaatttctc aaatctatca ttatattact atatttatga
     8101 aacataaaat tcatatcata catttcacat ttaacaaatt cattttgata actatattta
     8161 taaggttcgt attttgaaaa actcgactca aaatatttta ttttagcata acataatttg
     8221 tgtatgttga acaaaagtgc aagaacagac tccttaactg caaacttatg tgtaaagtat
     8281 tttatcgtgt ggtatttacc atgtttaaac tcccaaatta aatcctcatt taaagtaaga
     8341 gaatattttt ttataaggtt acgcgtatct agtgttaaat tttgtattga atgtattttc
     8401 ataatttatt ctcctcataa atatttaata agtattaaat tatagtgttt tattattaat
     8461 tgttcttaat tatattgtat ggtaaattaa aaaagtaata aaatagataa ttaatatact
     8521 agtaatatat gctataaata aaaacaatag ctaaaatagt aatatatatg tttttatgat
     8581 attttatgtt tagggttata tttgtaatta ttttaagctt ctaaaagtag atttgatgga
     8641 taacgtataa ttttagaagg atatgcaatt tgtttgataa gaacgtacat atgattaaat
     8701 taaaaatatt aagtaccaaa aagaagaaag atagtttctc cctttggtac ttaatatttt
     8761 gtatccttta cactaatttt taatatttat taatgatatt atttaatcta gagacttaaa
     8821 taaatttgcc atttctagcg cattcatagc acaatcataa cctttatttc cagcttttgt
     8881 acctgctctt tcaatagctt gttcaatatt ttctgtagtt acgattccaa atataacagg
     8941 taattcactg tctaaagata ctttggctat ccctttagag acttcactac atacataatc
     9001 ataatgagaa gtagcccctc ttataacaca gcctaaacag attatagcat catacttacc
     9061 acttttagcc attttttgag caacaagagg aatctcaaaa gctccaggta cccaaactat
     9121 ttctatattt tctggagaaa cgtcatgtct taaaagacag tcttcagctc ctgataaaag
     9181 ttttgatgtt ataaattcat taaaacgaga atttattata cctattttta aatcttttcc
     9241 aataagtttt ccttcatata tcatttttac ttcctccaat tttatttatg ttatagattt
     9301 atatttataa atatctttaa tttaattata aaaaactact tagtagaaaa atatcatcat
     9361 ataatcattt atataatatc taaaagatgt cccattttat cttttttagt ttttaaatat
     9421 agcttattaa agtcattaat ttcttcatta atagggattc tattttcgat ttttattcca
     9481 tatttctcta attgatttat tttatctggg ttattactta aaagatttat actttttatg
     9541 tttaaatttt ttaatatttg agcagccata tagaaatctc tcatatcttc ttcaaatcca
     9601 agcattaaat ttgcttcaac agtatcgtaa ccttcttctt gaagtttata agccttaatt
     9661 ttatttaaaa gaccaatacc tctaccttct tgcttcatat aaattaaaac tccacttcca
     9721 ttttccacaa ttttcttcat agaagattct agctgtaatc cacaatcaca ttttagagag
     9781 tggaacacat ctcctgttaa acattcagaa tgaagtctaa ctagagtatt ttctatattt
     9841 atatttccat aagtaagggc aatgtgttct tcatttgaat aagtatctct atatcctatg
     9901 atttcaaaat ttccatattt tgtcggaagg aaagcactag atactttttc tatagtagtt
     9961 tcatttattt tgcggtattc tataaggtct tttatagtga tgatttttaa attatgcttt
    10021 tctttgaaaa tcattaaatc atcaactctt gccattgtac catcatcttt cattatttca
    10081 cagataagac caatctcttt aaaacctgca agtctagcaa ggtctactga ggcttctgta
    10141 tgtccatccc tgacaagaac tccaccagtt tttgctatta aaggaaagat gtgaccaggc
    10201 gttttgaaat ctttacttgt agaattttca tcacagaata attttacagt ttcagctctt
    10261 tcatatgcag atataccagt tagagtatct gctccatcta tagtaacagt aaaggctgtt
    10321 tgaaatgtat ctgtattttc tctaaccata agaggtatat taagactttt aaattttttt
    10381 tcagtggcag gtagacaaat tagacctcta ccatatgtag ccataaaatt tattgattca
    10441 ggagtagcca tttcagctgc catcaataaa tctccttcat tctctctact ttcatcatca
    10501 actacgatta ccattttccc attttttata tcttcaatag cttcttcaat agtattaaac
    10561 atgtaattca ctcctttatt atttttaaaa accattttta aataaaaaat ctttatctat
    10621 atttgattta tggttactac tattagaatt taattccttg tagttattag ccataaaatt
    10681 attaacatac tttccaatta catcgctttc taaattaacg aaatctccaa cattctttgt
    10741 caagagaata gtgttaattt tagtatgagg tataatagaa accttaaata tttcatcatc
    10801 taaatatgat atagttaaac ttacaccatc aatagcaaca gaacctttga gtatcattga
    10861 ggataaaagt tttttatcag gtcgtatact tactaaaact gcattttcat ctttttcaaa
    10921 tttgcagata gttcctttac catcaacatg acctgttact atatgaccac caaatcttga
    10981 agaaagactc atagctcttt caagatttac cttgtcattt gcatttaata aacctaaatt
    11041 tgtggaacga attgtctcca tcataacatc ggctgtaaat tcattttttc cgaggtttga
    11101 aacagttaga caaactccat ttacggctat actatctcca agatttgtgc catctaatat
    11161 tttatttgat agtatggtta cttttccact cttaccatta gcagtaattt ttctaagtat
    11221 tccaacttct tctactatgc cagtaaacat cctaacctcc tatatgaatg tattaacaaa
    11281 aatttaattt aaaacatctg attctataag aatatcgcca tctatcaaag ttacattatt
    11341 gattgttaac tgtgttgaat ctgacaaatg atttattcct tctccagaaa cgaatgtagg
    11401 tgcatcttta ccacctataa tcttgggtga tagaaataat ttgactttat caactaactt
    11461 atttctaaag agactgtcat tgagagtagc accaccttca actagaacag aatcaatgtt
    11521 taactctcct aatttataca ttacaaactc caaatctact aagttgtttt ttgaaggaga
    11581 aattattgtt tcaacattat tttcttttaa catagataat tttatacttt ctttgcaaca
    11641 tgtgaaaact atagttttac tggttttatc tttaaccaaa ttactattaa gtggcaactt
    11701 taggtgtgta tctattacta ttcttgtagg atgagacact ttttcttgat tgagcctaca
    11761 agttagtaaa ggattatcgt tcaaaactgt atttatgcca accattatac tttggtattt
    11821 atttctgtat ttatgagtta gataccttga tgattcatta gatatccatt ttgattctag
    11881 tgattttgtg gcaattttac catctagact tactgctgat tttacaatac aaagaggtct
    11941 tttgtttttt atatgataaa aaaatgcctc atttaaatct cttgcttcat cttctaatat
    12001 tcccacagta acatcaatat tgttatcttt taattttttt actccattac cacaaacaag
    12061 tggatttggg tctaatgtag atattacaac tctcttaatc ttattttgta taattttatc
    12121 tacacaagga ggcgttttcc cataatgaga acatggttct aagtttacat aaagagtaga
    12181 gtcttttaga ctttgtttag cactgtttat agcatttact tcagcatgat tagagccaaa
    12241 tttctcatga tatcctttgc ctattatatt actatcttta acaattacac atccaactaa
    12301 tggatttgga tttacaaagc cttctccatt tttagccaat tcaatagctt ttttcatata
    12361 ataaatatct ttttcttttt gattcacaaa attcacaata tgtacacctc ctgtttaatt
    12421 ggataatttt taaacaaaaa accctgagaa ttaactcagg gtttagaata atctacaata
    12481 aatgtttaat aaatgtagaa caaaattgct ctacaaaaac actcattaaa tatcttcttc
    12541 catccagact ttactgtcgg ctttggaatt taaccaaatc aaccaagttt tacattggct
    12601 cgcgggctat accgccgatt gggaatttca ccctaccccg aagattaagt attaaattta
    12661 tatttagaat gtatcataaa ttattttact ttgcaaacta tttttttaaa aaatatatgt
    12721 taatatataa tatagaatgt ctataaactt tgaaatacca cgattgtgaa tataaaaata
    12781 aaaatttgaa cataatcagg aaagaagtct aattaattta aattaggaga gtaatatatg
    12841 aataaaaaac ttttagaaat actatcaaaa tactatggat atacaagttt tagaaaaggt
    12901 caggaatcta taataaattc tatcttaagc gaaaaagatg tgctagctat tatgccaaca
    12961 ggagggggaa aatcaatatg ttatcaaatt ccagctttga ttttagatgg aatgacaata
    13021 gtaatttctc cacttatatc acttatgaaa gaccaagtag atgccttaaa gactatggga
    13081 attagtgctg cttttataaa tagttcatta agtagcaaag aatttaatga aatattaaat
    13141 aatattagaa aaaataatta taaaatactc tatattgctc cagagagatt agatgcacaa
    13201 gagtttattg agctaataaa taataacaat gtaagtcaag ttgcgataga tgaagcacat
    13261 tgtgtatctc aatgggggca tgattttagg ttgagttata gaaggatttc tgattttata
    13321 aaaaatttgc ctaaaagacc tatagttaca gcatttactg caacagcttc agaagaggta
    13381 agaactgata taataaattt actgtgttta gaaaatccag attattatat aactggattt
    13441 gatagagaga atttatcaat aaatatagta aaatcatcta gtaaaaataa gtatatttta
    13501 gattatattc aaaatcataa aaatgagtct ggaattatat atgttgcaac tagaaaggaa
    13561 gttgaaaata tatataatgg tttatctaag agaaatatat ctgtatcaaa atatcatgca
    13621 ggtttatcta aaaatgaaag aagtaaaaat caagaagatt ttataaatga taatgtggat
    13681 ataatggtag caacaaatgc atttggaatg ggtattgata aacctaatat aagatgggtt
    13741 atacattaca atatgcctca aagtatagaa aactactatc aagaaatagg tagagctggt
    13801 agggatggag aaaaaagtga gtgtgtactt ttattttctc caggagatgt acatatacaa
    13861 aaatatttaa ttgatattgg agttgaaaat cctgaaagaa aattatttca gcataagaag
    13921 cttcaataca tgattgactt agtttacagt aattcatgct atagaaagac aatattgagt
    13981 tattttggag aaaattatat agacaattgc aataattgta gcaattgttt agtagagggt
    14041 gaaatagtag ataaaactat agatgcacaa aaagtcattt catgtgtagc tagaatgaaa
    14101 agaagttatg gtgttactac tattgtagat gtgcttagag gttctaagaa taaaaagatt
    14161 ttacagcttg gattaaatac tttgtctaca tataacatta tgagagatta ttctagtgaa
    14221 gacttaaaga attttgtaaa tacattggtt tctcatggtt ttttagattt agttgagact
    14281 ctaggcaatg gaaatagagg tagttttcct actataagat taaatgagat gtctatgaaa
    14341 gttttaaaag gtgaagtgaa agtagagttt aaagaaattg ttatgacaaa atcattggaa
    14401 gtagaggatg agttatataa tgctttaaga gaacttagac attcaattgc aagtgaagaa
    14461 aggatagctc catatatggt atttggagat ggtacactta gagccatgtc tagtagttat
    14521 ccaattaata aagaagaaat gctagatatc tctggagtag gagaaattaa ataccaaaaa
    14581 tatggtagag attttgaagc agtaattaaa gaatatgtcg aaaaaataat ataaataaaa
    14641 aaatagatga aaaaaatgtc gattcaaaga acgttaatag tgatttctta gaggttaata
    14701 cagataaagt tttatatgaa aaactgttag tcattagaga agaatatgca acaaaggaaa
    14761 aattacctgc atatatgatt ttagccaata aggctttgaa agaaataagt ggaagatatc
    14821 cacttgatga agaacaatta aaagatattt ctggcatagg tggagtaaaa tttgaaaaat
    14881 atggagctag tattttggca gaggttaata aatatgtaaa agaaaataat attaatgtta
    14941 tttgggagaa aaagggcaaa aggaagttaa tattagatgg agaaagtaga aaaaataatg
    15001 aaatagcatt agatttgcta aatcaaggaa aagatataca aagtatcagt caagaaatag
    15061 aagtttcact gtcaaccatt attggatatg tgcatgacta tgtaaaggat ggacatagta
    15121 tatcatttga attaggacta gaaaattttt ataatgaaga taaaaagaag atgattttag
    15181 aagtttgcga aaaagttggc tatgataatt taaataacat aaaaagaaaa cttccagatt
    15241 caataaagta tgaaaatata agagctgtga tacttgactc ttatcttgaa aacttaaaaa
    15301 ataatattat agcagagtaa tttttaactt aataaaaaat aaaatttgta ttattgtgtt
    15361 gaactaataa ttttgctgtg ttatactgaa tttgtaaaaa taatgaaaaa ttagagctag
    15421 gggcgctaga attggctagc tgagagataa agccgtaact tacaaaccct aataacctga
    15481 tcaagataat gcttgcgaag ggaagctttt taatataatt ttattatata aataggcaaa
    15541 ttattcaatg ttaggagaaa tttgtctaag tgtatatacg attataagag tgctttccaa
    15601 acgaggaaaa gcactttttt tattttcaaa aactataact ttaatcttgg aggattgtaa
    15661 tatgaactat acaacacaaa tggatgctgc tagaaaaggc ataataacta aagaaatgga
    15721 aatcgtttct caaaaagaac aggtagatgt aaatgaatta agagaattaa tagcaaatgg
    15781 acaagttgtt ataccagcta ataaaaatca taaatcttta agtgcagaag gtgtaggtaa
    15841 aaatttaaga acaaaaataa atgtaaatct aggaatttca agagattgta aggatataga
    15901 aaaagaactt gaaaaagtta gagtagcaat agacatgaaa gctgaagcta taatggattt
    15961 aagtaattat ggcaaaacta gagaatttag agaaaaggtt gttgaaatgt cacctgcaat
    16021 gataggttca gtacctatgt atgatgcagt aggatatttg gaaaaagaat taaaagatat
    16081 aacagaagaa gagtttttaa atgtaataag acaacatgct attgatggag tagactttat
    16141 tactatacat gcaggtctta caagaagtgt ttgccaaaaa ataaaaaatc atgagagatt
    16201 gactcatata gtatctcgtg gaggttcttt attgtttgca tggatggaat taaataataa
    16261 ggaaaatcct atttatacta attttgataa aatattggat atatgtgaag aatatgatgt
    16321 tacattgagt ttaggagatg cttgtagacc aggttgtata aaagattcta ctgatggtgt
    16381 tcaaattcaa gaacttgtag tattaggtga acttactaaa agagcatggg aaagaaatgt
    16441 acaagtaatg atagaaggtc ctggacacat ggctattgat gaaatagaag caaatgtagt
    16501 attagaaaag agattatgcc atggagcacc tttttatgtt cttggaccat tggttacaga
    16561 tatagcacca ggttatgacc atataacaag tgcaatagga ggagctttag catgtgctaa
    16621 aggagtagat tttttatgtt atgtaactcc agcagaacat ttaagattgc ctaatttaga
    16681 tgatatgaaa gagggtataa tagctgcaaa gatagctgct catgcaggag atatagctaa
    16741 aaatgttaaa ggtgctcgtg aatgggataa taagatgagt aaagctagag ctgatttaga
    16801 ctggtgtgag atgtttagac ttgcaataga ccctgaaaaa gctaagagat acagagatga
    16861 atcaactcct actcatgagg atagttgtac tatgtgtgga aaaatgtgct caatgagaac
    16921 agttaagaaa atattaaaca atgaggaact taatctaata taatttttgg ctagtatgac
    16981 tggaataaaa ataagctgaa aagaggaggt gatttagtga aggttaatgg gaaggaaatt
    17041 gaatttgaaa aagatttgac tgttatagat ttattaaata aatataattt aaaaagtgat
    17101 agagttgtag ttgaagtgaa tttagagata attgaagaat ctaactacaa tacatatgta
    17161 ttaaaagacg aggacattgt agagcttatt agctttattg gaggtggtta aaataaaaat
    17221 atttgtaaat gaattattaa cagatgttga ggaaaataca actgcttata aagttagaga
    17281 tagctataac aataaatgtg atgtattggt gttaaatgga tatccaatta aaacagatat
    17341 gaccctttgt gaaaatgata aattaacact tatacaaaaa ggagttaagc catcacttga
    17401 tgaattggaa agcttgttga ttgctagaca tactccaaat atacataata aattaaaaaa
    17461 agggaaagtt gctatattgg gattgggggg acttggttcc aatatagcta tctctttagc
    17521 tagaatagga gtgggagaac ttttacttat agattatgat gttgtagaac catctaattt
    17581 aaatagacag cagtatttta tagatgatat aggaaaacta aaaactgatg ccatgataga
    17641 aaatataaaa aaaataaatc catttataaa attaaataaa cgagatattt ttttaaacaa
    17701 gaacaatatg gatactatta aggattcaga tttaataatt gaggcctttg atgatgcttc
    17761 atgtaaagca caggtatgta attatgtatt aataaattta aaagataaat atttaatagc
    17821 atcatctggt atggcaggtt actatgattc aaatataata actactaaga agataaaaga
    17881 caggttttat atatgtgggg attttgtgaa tgaagctaaa tttggtgaag ggcttatggc
    17941 tccaagggtt gccatctgcg caaatcatat ggcaaatttg gcaacgcaaa tacttataga
    18001 tatgtaaata ttaaaattag atataaaaaa gattgaatat taaaaaaatt taacgtggag
    18061 ggaaataata tggataaatt ggtacttggt ggacatgaat ttaatagcag attattggtt
    18121 ggaacaggta aatatggttc aaataatata ttgcctgagg taataaaaga aagtggaagt
    18181 gaaataataa caatggcact tagaagagtg gatttagaca ataaacaaga aaacatatta
    18241 acgtatattc caaaagagat gacaatactt ccaaatacat caggagctac taatgcagaa
    18301 gaagctgtaa gaatagctag aatatctaga aaaatgggat gtggagattt tataaaaata
    18361 gaagtaatat cagatactag atatttactt cctgataatg aagaaactat aaaagctaca
    18421 aagatacttg ctgatgaggg gtttatagtt cttccatata tgacaccaga cctttatgct
    18481 ggaagaagac ttatagaagc taatgcagcg gcagttatgc cacttggagc tccaattggt
    18541 tcaaatagag gtcttcaaat gaaagaaatg ataagaataa tgatagatga gttggatata
    18601 ccaataatag tagatgcagg tattggaaaa ccatctcaag ctatggaggc tatggaaatg
    18661 ggagctgatg cagtacttgt caacactgct atagcttcag caggagaccc tgtacaaatg
    18721 gctagagcat ttaaattagc tgtagaaggt ggaagagaag catatattgc aaaaacagga
    18781 aatgtgtcag aatttgcaaa tgcatcttct cctttaacag gttttttagg caatttatag
    18841 aaagtggtga ttggtgatga gtttttatga tgtaatagaa aaatataggg attttgattt
    18901 tgatggatat ttaaataatg ttactgataa tgatgtttta agaagtttat caaaagacaa
    18961 gcttgaagat tttgatatat taaatttgct ttctaaaact gcagtaaaac atttagaaga
    19021 tatggcacaa aaagcacata aattaagtgt tcaatacttt ggtaaaacag tatgtttata
    19081 tacaccaatg tatatagcaa attattgtgt taatcaatgt gtttattgta gctataatat
    19141 aaagagtgga ataaaaagaa aaaaattaac tatggatgaa attagagaag agggagaagc
    19201 aatttcaaaa gaaggtttca aacatctact tgttttgact ggagaaagta gttttcactc
    19261 aagtgtagaa tatataggtg aggctataga aatattaaga gagaaatttc cctctatagg
    19321 tatagaagtt tatccaatgg aagtagaaga atataagtac attgtagata aaggtgttga
    19381 aggattaaca gtatatcaag aaacttatga cgaggaaata tataagagag tacatataaa
    19441 aggcccaaaa tcaaattata aatttagact agatgctcct gaaagagggg caaaagcagg
    19501 tatgaggact ttatcaatag gtgcactttt agggctaaat gattttagaa aagaaacttt
    19561 ttttacaata ttgcatggga aatacttaaa aacgaaatat cctcatattg agctttcata
    19621 ttcaacacca agaatgagac cttttaaagg gtgttttgag gaattggtag atataagtga
    19681 tactgattta gttcaagcta tggtatgtat gagattattt gaccctcatg cagctataaa
    19741 tatatccact agagaaaatt tagaaatgag aagtcatata atacctttag gagtaactaa
    19801 attgagtgca ggtgtatcta ctgatgtagg aggtcattct caagatgaac atgatactgc
    19861 tcaatttaag ataaatgatg aaagtactgt taaagatgtg gagaaaatgc taaactcaat
    19921 tggttatcag catgtgttta aagattggga aagattttaa atgtatctga taacaaacag
    19981 aaaattatgt agtgaggaac gatatttaga agttattaaa gagtctatat tgagtggtgt
    20041 agaaaatata ataataagag aaaaggattt agaatatcaa gagctaagaa aattatatat
    20101 gaaaataaaa actaaaatca attgtataga ttttcaagag caaatatcag atgaaagttt
    20161 gaaaactaat ataaatcaaa aagagtgtag aaataaattc aaggttaatt ttataataaa
    20221 tagtaacatt gaattttttg aaaaagtgga ttgtcaaggt atccatcttc cttttaaatt
    20281 attcttgaat ttaattgaaa ataagtataa ttttaatgaa aataaaatac taggattatc
    20341 tttacataaa gtagaggaag tagattattt agaaaaatta ataagaaatc aaaatataaa
    20401 aatagattac ataacattat cacatatata tgaaactaaa tgtaaagaag gattaaatcc
    20461 aaaaggaatt gagcttttaa aagaagctaa aaagattact gatattaaaa taattgcact
    20521 tgggggaatt ttaccatcta atgtcaagga aacattaaaa tactgtgatg attttgctat
    20581 aatgtcaact ataatgaggt ctaaagatat taaaaaaacc atatcaaatt ataatgaaaa
    20641 gttgaattag taaatatagt atattgaatt tttaacttaa ataaaaataa aaaagttatc
    20701 ttttaaattt taaaaggtag cttttttatt ttatatcttt gctaataaaa aattgtaaaa
    20761 agggaaaact ttagtcagag agattaaaca atatcctaga aagatttaaa atttagtaaa
    20821 gtatggagga cattataatg actgacagta aaaagaaacg atttattata ccacacactt
    20881 ttacaataat atttttacta atagtcttga tggcaatatt gacttggata gtaccaagtg
    20941 gtgaatttag tagagaagat gtagaaggta gaatggtagt tgtaccaggg acttatcaaa
    21001 gtgtagaaag taatcctcaa ggatttgcag atgtttttaa agcaccaata gaaggcttta
    21061 tcgattcagc tgaagttgta ggttttgttt taatagttgg aggtgctttt ggtattgtaa
    21121 ataggactgg agctatagaa gcaggaatag cttcggttat ctctaagttt aagggaaaag
    21181 aacttcttat aattccaata tcaatgattt tatttggact tggaggtaca acatttggga
    21241 tggcagaaga gacattacca ttttatatga tatttgttcc actcatgaca tccttgggat
    21301 atgattctct aacagcaata gcaattgtat ttataggagc agcagcagga gccgccgctt
    21361 caacaataaa tccattttca gttggtattg cacaggcact agctcaactt gctcctggtt
    21421 caggagtaac ttacagagta gttattttta tagcatatat actaataagt atagcatttg
    21481 ttatgtacta tgctaagaag gtaaaggaaa atcctgaaag ttcaatagta tatgaaatag
    21541 ataaaaataa taaatcaata cttaatcaaa cttcaacaga aataaaagaa tttactttta
    21601 aagaagtatc tgttcttgct atatttggaa tagggatggc aataatgatt tggggtgttt
    21661 taagtcaagg atggtacatt ccagaaatat caatgtgttt tgctgcaata ggtgtgcttt
    21721 ctggattggt tggtagatta tctcaaattg aaatctcaga aagttttata gatggagcaa
    21781 aagatttagc atctgcagct cttgctatag ctttggctcg tggaatcgta ataattgctc
    21841 aaaatggatt tataatagat actatattaa attccgcagc agcctttcta ggtgggcttc
    21901 caaaggttat atttgtatat ttatcatttt tcttggaagc tgcattggca tttttaattc
    21961 catcatcttc aggtcttgct tcacttacag tacctgtact tgctcctctt ggtgatttgg
    22021 ttaatatatc ggctcaagtg atagtaaccg cttatcaatt tggtgtgggt cttacaaact
    22081 ttataactcc aacttcagga gtactgatgg gagcacttgc agtagcccat attccatttt
    22141 caaaatgggt aagatttata atgcctatct tgttgatttt gacagctgta tctctagtct
    22201 ttttaacaat aggtatttat atagggtttt aattaagtgt agttttattt aagatattta
    22261 gtaatggtat aaaatgataa tatgttttca ataaattgcc actaatttaa ttagtggtaa
    22321 tttgtttttt gtatttaaaa ataaatatat ttacttgtat ttt
//
LOCUS       DAMJWU010000055        22157 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMJWU010000055 DAMJWU010000000
VERSION     DAMJWU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.055"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            588..1895
                     /locus_tag="PC460_002282"
     CDS             588..1895
                     /locus_tag="PC460_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601163.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1981..2243
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2369..3172
                     /locus_tag="PC460_002283"
     CDS             2369..3172
                     /locus_tag="PC460_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2601164.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3210..3821
                     /locus_tag="PC460_002284"
     CDS             3210..3821
                     /locus_tag="PC460_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2601165.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3853..4524
                     /locus_tag="PC460_002285"
     CDS             3853..4524
                     /locus_tag="PC460_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2601166.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4822..5184
                     /locus_tag="PC460_002286"
     CDS             4822..5184
                     /locus_tag="PC460_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF2601167.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            5620..6321
                     /locus_tag="PC460_002287"
     CDS             5620..6321
                     /locus_tag="PC460_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF2601168.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            6591..7409
                     /gene="larE"
                     /locus_tag="PC460_002288"
     CDS             6591..7409
                     /gene="larE"
                     /locus_tag="PC460_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF2601169.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            7464..8612
                     /locus_tag="PC460_002289"
     CDS             7464..8612
                     /locus_tag="PC460_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF2601170.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            8748..10136
                     /locus_tag="PC460_002290"
     CDS             8748..10136
                     /locus_tag="PC460_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2601171.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            10165..10863
                     /locus_tag="PC460_002291"
     CDS             10165..10863
                     /locus_tag="PC460_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2601172.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            10866..12299
                     /locus_tag="PC460_002292"
     CDS             10866..12299
                     /locus_tag="PC460_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2601173.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            12321..13058
                     /gene="truA"
                     /locus_tag="PC460_002293"
     CDS             12321..13058
                     /gene="truA"
                     /locus_tag="PC460_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2601174.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    13169..13376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13629..14828
                     /locus_tag="PC460_002294"
     CDS             13629..14828
                     /locus_tag="PC460_002294"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF2601175.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            15075..16121
                     /locus_tag="PC460_002295"
     CDS             15075..16121
                     /locus_tag="PC460_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF2601176.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            16229..17392
                     /locus_tag="PC460_002296"
     CDS             16229..17392
                     /locus_tag="PC460_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2601177.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(17481..17861)
                     /locus_tag="PC460_002297"
     CDS             complement(17481..17861)
                     /locus_tag="PC460_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF2601178.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(17864..18523)
                     /locus_tag="PC460_002298"
     CDS             complement(17864..18523)
                     /locus_tag="PC460_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF2601179.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(18629..19603)
                     /locus_tag="PC460_002299"
     CDS             complement(18629..19603)
                     /locus_tag="PC460_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2601180.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(19643..19990)
                     /locus_tag="PC460_002300"
     CDS             complement(19643..19990)
                     /locus_tag="PC460_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601181.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(20208..21164)
                     /locus_tag="PC460_002301"
     CDS             complement(20208..21164)
                     /locus_tag="PC460_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF2601182.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            21607..21975
                     /locus_tag="PC460_002302"
     CDS             21607..21975
                     /locus_tag="PC460_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2601183.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         8792 a         2235 c         3648 g         7482 t
ORIGIN      
        1 ctaatttata tgtataaatt aattatgaaa aatttatata agtaaaatat attaacataa
       61 aggtaaaatt ttgtatacta aaagggaata tttacagttt aatggcagtt ttaaaataat
      121 aaattaaagc tgtcattttt atttaaaaat ttttagaaaa agtacaatag tgttaatgtg
      181 tgaattagat aaggtgtata taatagttta gatgatatga tataaaatat gttgtaaatt
      241 aaatgttttc tttgacaata tatacacaag gatgataata tctatatgaa taaaagatag
      301 tgtgtaattt gataatatgt aattataaca ttatgatata attacaataa agtatgtaat
      361 aatctgtaga aatttcaagc tagttatcta agttttcgga taactaaatc taaacggaat
      421 acataatgta ttaaataaat gatgatacta aaatgatact atttttatag aaatttattg
      481 aggtatataa ttaatgaaaa taatatatta aattatataa attaaattat ataaattaaa
      541 ttatataata tattgagttt tacataaaag caaaggtggg aataaaaatg aaagaaattg
      601 ctgatgctat aacatcagag tatttaagct atggtatcaa tgaagatatt agtatctatg
      661 aaggtagatt ttgcatatat ttagataaaa aatatagatg taatgggaaa atttattata
      721 aaatgacacc tccaatatct ataagtttta aagcagacat aggttgtgtt gaagagatag
      781 acaatgaaga tgacaattta gctttagatt atgataatgc tattcttgaa gttcatggat
      841 ataaaattat ttctataaca ataaatactt taagtgaatt tagtgtagaa ggttatatca
      901 atgatgactg cataaaatct aagaactcct atgttgaata tgtagatttt aatataataa
      961 acttagataa aatacctgga aaactcatta aatacaatga taaggtatac gcaggtagaa
     1021 ttgaatttga tataaatgat tatgttgtaa ctatagacaa gcgatatgat tatagaaaag
     1081 aattaaaatc agaattaaag tctaaatctg gagcaataat aactcatata ggtaggatta
     1141 gaagaaaaga tggtagaatt tttagaacaa ataatacaat aaatctttta gataggatat
     1201 caacagcttt gagttttatg tgtggaagat atgtagggtt ttgtttggcc aaagggtata
     1261 gaagtggtaa tgaagtttat agaatatgga atgaaaatca aatatctcct tttagatatg
     1321 taccaacatg gtcagataca ctttcaaatt attataatat ggaaaaatat ataagcctta
     1381 tgtgtaaaaa gctagaagat ttttattatg gttctgctat aaagagtgtt gttgattggt
     1441 acatagaatc tcttggaagt gctaccatgg aaaacaatat aatatctgta caaattgcac
     1501 ttgaaacttt gtcatatgta attctagtgg aacaaaataa gatacttaca gatgaagtat
     1561 ttgactgtaa tttggcatct aaaaatataa gactcttact tgatacatgt aagataccat
     1621 atggtaaaca tgaattaaat atatttgata atataataaa aaataaattt gatgatggag
     1681 tggatttagt tatttattta agaaatagca tagttcatcc aagtagaaag actcatagag
     1741 ctgtgttaga ggttgaagat atttggaata taatttcaat aggaacgaga tatattgaac
     1801 ttgttttact ctttatttta ggttatagag gagaatattc aaacagatta gtagaaagat
     1861 gttatggtga agtggaagtt gtaccttgga actagatttt tatatttatt ttaaaatata
     1921 tattgacata tattgcctta tgctataaaa tcaatatcat aataataatt cagaataaca
     1981 ttaaatgcta agaaaagaaa tagtaaaagt agttaatctt aaacagagag tttccgattg
     2041 gtgagaggag caatagattt tactttgaac acatctttga gcaggactct gaaagagtag
     2101 aaatacttta ttaggagatt tccggaatcc ttgcacccgt tatagtgcta gagtataata
     2161 attttattgt acttaaagag gttagtgtcg tgaggcatta atgaacagag gtggaaccac
     2221 gagagttaac tcccgtcctc tagcagaagc tagaggcggg agttttttaa tataattttt
     2281 taagcgcgca gagaattata ttgcaataga tacacttttt aaaaaataat caaaatattt
     2341 taaaaattaa aaggcgaggg agaatattat gaaaaatata ttaaaaaaag taggaatatt
     2401 tacaattatg ttaggtttat taggtggagt tgtaggttgt tcaaaaccag ataatgaaaa
     2461 agataaggat gcttcaaagg aatctaaaaa agaagtagtt gtagggtttg ataatacgtt
     2521 tgtaccaatg ggatttttag atgaaaaggg agatacagta ggatttgacg tagacttagc
     2581 taaagaaaca tttaaaagac ttggaatgga agttaagttt cagcctatag attggtcaat
     2641 gaaagaaact gaattaaatg attcaaaaac tgtagatgta ttatggaatg gatattctat
     2701 aacagacgaa agaaagaaaa tagtatcata tacagaacca tatttacaaa ataagcagat
     2761 aatagttact ctaagtgact caaaaataaa ttcaaaagct gatttaaaag ataaagaagt
     2821 tggaacacaa caaggttcaa cagcacttga tgcagtggaa aaggataaag attttatgaa
     2881 tagcttaaaa ggtggagcac cagttcttta tgatacttat gataaagcac ttagagattt
     2941 agagataggt agaactagtg cagttgttgg tgatgaagtt ttaattagat actatatggg
     3001 acaaaagggt gaagacaaat acaaggtatt aaaagacgat tttggattag aagattatgt
     3061 tgtagcaact tctaaagaaa atccagaact ttgtgagaaa ataaatgaaa cattaaaaga
     3121 aatgaaaaaa gatggaactt ttgacaagat ttatgataag tggtttaaat agaagataag
     3181 ttagctgtta agaagggggc actaaaggca tgttacaagg tttagagata gttatagcaa
     3241 tgttttgtat aacattgata gtatcaattc cattggggat tggagttgca tttttaagat
     3301 tatcaaagaa taaattagta agtggtatta cacaatgtta tatattgata atgagaggta
     3361 cacctttact tttgcaaatg atagtcatat tttacggatt acctctttta ggaattgtat
     3421 ttgacagatt tacagctgga gtagtagctt tcttcttaaa ttatgcagca tattttgcag
     3481 aaatatttag aggtggaata cagtcaatcg atagaggaca atatgaagct tcaaaagtac
     3541 taggatttga taagtttact atgtataaga gagtaatctt tccacaagtt tttaaaagaa
     3601 tactagcacc tatttcaaat gaagtaatca cattagtaaa agatacatct ttagtatata
     3661 tattaggatt aaatgatatt ttaagaatat ctcaaatagc aatgaacagg gaagcaagtc
     3721 tgttaccatt atttgaggca ggagcgatat atcttatatt tgtagctata cttactaagg
     3781 gatttgaatt acttgaaaaa aaatattctt attatagata aagttacaat attataggag
     3841 gaggtttttt atatgttgaa aatcaaaaac ttaaataaat catttaaaaa aaatagagta
     3901 cttaaagata tttcttttga gttagaagaa ggtcagattg gagttttact tggtaagtct
     3961 ggtgcaggaa agaccacaat attaaggtgt ataaatggtc ttgaagaatt tgatagtgga
     4021 gagattataa tagataatga ggtaataaaa aataagagag atatggcaaa aatacgagga
     4081 aaaataggta tggtttttca aaatttcaac ttatttcctc atatgacagt ccttgaaaat
     4141 ataattgaat ccccagttaa tgtttttaaa gttcctagaa aagaggctga agagagggct
     4201 agagaacttt taagattagt tgatttagaa gataaattaa attcttatcc atttgaattg
     4261 tcaggaggac agcaacagag agttgctata gctaggtcat gtgcattgat gccaaaagtc
     4321 ctttgttttg atgaacctac ttctgcactt gatattgata ctattcaaag agtagtcaat
     4381 ataatgaaca gacttaaaga caaaggtatg acaatattaa taattacaca tgatgttgtc
     4441 ttctcaaata atgttgcaga taaaattata agtataaaag atggtatagt agaaaatgtt
     4501 caaataaaag aaaaaattgt ttagagtttt ataaatataa actagggtag aattttataa
     4561 tataaataaa gggcaagtaa aatcaaatat actatgccct tttgttatct caaaatttaa
     4621 tcttatattt tatgtttaag ttacattata atttataata aaaataaaag gactatttaa
     4681 atatataatg tgaataatgt ttaataattt gaaatatgat aattttttat gttgaaagct
     4741 tataatgatt atgatataaa ttggttatag tatacaattt atgggtataa caataaaaaa
     4801 ttagaaagga atttttataa aatgattaaa ttatatggat atacaaaatg ctctactgtt
     4861 aaaaaagcta aaaattggct taaggaaaat aatttagaat ttgaagatat agatatggtt
     4921 caaaaccctc ctagtaaaga ggagttaaaa tcaatttata agactagtgg atatgatata
     4981 aagaaatttt ttaatacaag tggtatgaaa tatagagaat tgggtcttaa agatattgta
     5041 aaaactgaat ctgatgataa acttttagaa atattggtaa gtgatggtat gcttattaaa
     5101 agaccattac ttttggatgg taaaaatgta ttattgggtt ttaaagaaga tgtatggaaa
     5161 agtactttgc taaaagaaga ttaggaagag ataggtgtgt tgattaaaat tattaattaa
     5221 tacacctata aatttatgta ttaaatctaa tctttataaa tattaattag caaattcaaa
     5281 tcaaaaaaat tcatatcaac aaacatatta cttcttatta caaattatat catatgtttt
     5341 atattaaaat attataatat tacaacgaat tgttacaaaa ttacttatta ttaaccatga
     5401 acatttgttt tgtgctatca tatttatata agggaattgt cataaaaaat atcttgttca
     5461 tacagtttta tatagaaaaa agaaatgaat cttttcttta tataagtata ttttgtaaat
     5521 tgcctctata tttatagttt aaataatagt ggtattaaaa atctgacaac aaaaataaaa
     5581 ctttcaaaaa attaaatcta aaaagaatag gagataaata tgagtattga agatttatat
     5641 gattttgaat gtgttccttt gaacaatgag attgatattg atgttttatt tgaaaagatg
     5701 atagaagatg gaaaagaaaa agaatataca ccaattatag tcattgatga aaaagtaggg
     5761 ctattaagaa aaaatataga tatgattact aaagagtatg gttctcttga aaactataga
     5821 gagtattgtt taacaaaata taatgaaatt gatgtaaatc aattttttga ttttaggaag
     5881 agtgagattt cagaattatt aaaggagcta attgatttag aagatgattt ttatgattat
     5941 aaacatttta gacccattat gaatatagat atatttcatg aatataacaa agtttacata
     6001 gctaaaattc caacaattaa accatatgaa gtatttgcat acatacctat tggcggattt
     6061 aatgattgtc ctagagatga agaacatata gctattgcaa aatattggta tgaaaagcat
     6121 ggaatctttc caatcgcaat tggatgtgat acagttcaat attctgtaaa aaatttaact
     6181 aaagatggta aaaaatttga tgacttatgt attgaattag tattttattg tgaggatatt
     6241 attatacaag ggtatgaaac cttaaaggct cttaaggatg tattaaaacg ctctacaata
     6301 gtgctttttt ggtgggattg atattctatc atttagatag tattaaattg ttagtcacca
     6361 agaatccact tgctttagtt atagggagtg tgtcaaacta ggtttttata cacagtgtag
     6421 catagtaata tgtttaaatt acaaaggtta aaaaaatact ttaatatttt taatattata
     6481 attaatagat ttaaggtaat tataatttat aaaataaata ctttatatgt tataatcatt
     6541 aagaatgtaa aaaaataata tatagtttta tagttcatat ttttccagat atggaggtaa
     6601 attttgtgaa ggaaaaagaa aaattagata aattaaaaaa aatgttactt gaattaggca
     6661 gtgttgtagt tgcttattct ggtggagtag atagtaattt tttgcttaag gtcgcaaaag
     6721 atacattagg tgaaaatgtt atagctgtga ctattcatgc aatgatgcat tcaagtaggg
     6781 aaattgaaga ggctaaacaa tatactcaaa attttggtgt taaacatatc atattaaata
     6841 tagaaaactt tgatttaaaa gaatttaaag aaaatggtgt tgatagatgt tatcattgta
     6901 aaaaatatat attctctaaa ataaaagagg tagcaaaaga acataatata aagtacatag
     6961 tagatggaac aaatatagat gatttaggag actatagacc tgggcttaag gcattgagtg
     7021 aattaggagt aattagtcca ttaaaagata gtagtttaaa aaaagaagaa attaggtcgc
     7081 tttcaaaaac attaggatta aatactttta ataaaccatc atttgcatgt cttgcgagta
     7141 ggatacctta tggagttgaa ataacagatg aaaatttaag aatagtagaa aagagtgaag
     7201 aatatctatc caatttaggt ttttctcaat ttagagtgag aatgcatgga gatatagcta
     7261 gaatagaagt tggacaggaa gagctaggta aattttttga aaataacaat tttaataaag
     7321 ttgatactaa attaaaaata tttggcttta aatatgttac actagatatg tcaggatata
     7381 aaatgggaag catgaattta aatgtgtaga ttattttaaa aataaataag ttttatagtt
     7441 aatataagga aacaggaggt tttatgaaaa attatacgtt aatatcgcca tgtttttttg
     7501 gaatggaaaa aatgctggct agagagatta ctaatttagg atatgaaata ataaaaacag
     7561 aagatggaag aataacatat aaaactgatg agtttggaat agcaaagtca aacatgtggt
     7621 tgagatgtgc tgaaagagta catcttaaaa ttgctgaatt tgaggcaaaa agctttgatg
     7681 aattatttga aaatacaaaa agaataaact ggtctagata tataccttat ggagctcaat
     7741 ttcctatatc aaaagcttcc tctataaaat caaaattata cagtacacca gatgtacaag
     7801 ccatagtaaa aaaggctata gtagaaagct taaagaaaag ttatttggag gatggtttgc
     7861 ttaaagaaga taaggagaaa taccctattt ttgtatttat acacaaagat aaagtaacca
     7921 tttctataga tacaacagga gatgctttgc ataagagagg atatagagaa aaggcaaata
     7981 aagctccaat aagagagact ttagctgctg ggcttattta tttaactccc tggaaagcag
     8041 gtagagtttt agtagaccct atgtgtggtt ctggtactat attgattgaa gctgctatga
     8101 ttggtataaa tatggcacct ggcttaaata gagagtttat atcagaaaag tggagaactc
     8161 ttgataagaa aatatggtgg gatgtaagaa aagatgcttt taataagata gataatgaat
     8221 ctaaatttaa gatttatgga tatgatatag atgaagaatc tatagatata gctagagaaa
     8281 atgctgaaat agcaggtgta gatgaatata tagagtttaa tgtaggagat gcaactcaat
     8341 ttaaaagtga agatgagttt ggatttataa tcactaatcc tccttacgga gaaagattag
     8401 aagataaaga cagtgttaaa cagttgtata aggagcttgg ttatgcattt agaaaattaa
     8461 aaaattggtc gtattatttg ataacttctt atgaagactt tgaatatgag tttgggcaaa
     8521 aagcagataa aaaaagaaaa ttgtataatg gtatgttaaa aactaacttt ttccaatatc
     8581 cgggtcctaa gcctcctaga aataataaat aagttttaca tgatttattt gtaaaatata
     8641 aaattttatt aaaataagta aaacattatg atgccctgaa acgtctaatt attgtgagga
     8701 gatttttgtg aacaaattta aagatttata ggattaggag gagttaaatg aaagtgttaa
     8761 agcttattac tgtagtagcc ctgatgatgt tatttgttac tggatgtaat ataaaaactg
     8821 aatctccaga acaattagcg aagatacctg actatgatga tacaaaaaaa gtattgtatg
     8881 atggtattga ccagttacta aaaccagatt catctatgat attgccttct aatacaaaag
     8941 aagtaggaaa aataaataag gtagatttaa acagcgatgg aattgatgaa ttagtagtat
     9001 ttgaacaaaa agaagattta agtaataatg tgagtcaggt agggtttgta accttgagtt
     9061 acaatggaga aaaatacgta cttggagacc attttttaga aaatggcgaa tcaatagaat
     9121 atgctaattt ttatgatttg gactcagatg gatataagga agttatctta ttggttaaat
     9181 ccaaagacaa aacgaatatg catatatata aagtagtaga taatgaaata acaaaaatat
     9241 atgacttgga tgcatcatgg cttcaaaata aagaagattt taatgacatg aaagtaaaaa
     9301 ttggatatat agatggagat gacaagttag acatattgat gcttcatttg aataataaga
     9361 ctaatgaaat gtttgcaagt gttgcaaatt ttgatgggaa aatgaaaatc aaagattctg
     9421 ttaagtttga aaatgtaaaa aacttaagtg aattatatat aacattaggt aatgttgctt
     9481 catctgttgg aaattcttct gcagctataa aaggtattgt acttgatatt cctatattga
     9541 aagataataa ctatattacg cagatacttt atatgaaaga tgggaaaatt aacaaagctt
     9601 ttagtgatta tgataaaact attactaaat cttattatat acctgtagat gatattgaca
     9661 aagataagat tatagaaata cctatagttg caggcagtac aggtaataat aaaaatactt
     9721 atagttctaa atcatctgca actataagtt ggtatagatg gaatggaaaa gaaggtgctg
     9781 actcaagttt aatatttaca agtcagatat attataatta taaatataat tttaaattat
     9841 ttattccaaa taatttattt gataaaatac ttgttgaaca ggaatttgtt ggagaaaaag
     9901 cagtatttaa attttactat tttgactatt cagaccgtaa gaatttattt aatatagtag
     9961 tagagagtaa aaataaactt gaagatagaa aaaatacagg tactcaaaat tctattgtac
    10021 ttcaagagag cgatgaatat actttcttac tagttgtaaa caatgcaaat gaaatggata
    10081 agctagatat gactatagac gcattaaaag aatatttctc attaatatat ggttaatatt
    10141 atactcaatt ggaggtttta ttttatgaag gaaaagatac ttatactaga agatgaaatt
    10201 ggtattagaa gttttgttag tataaactta aaaagggaag gctatgaaat agttgaggca
    10261 ggtactggaa gagaagctat agaaaaaatg acaacagaaa aggatattac aatagctctt
    10321 ttagatgtaa tgcttccaga tataagtggt atagaagtat gtaaatttat aagggagaat
    10381 tttgaccaag taggtataat aatgcttact gctaaagctc aagaagatga taaaatagaa
    10441 ggatttatct caggagcaga tgattacatc ataaaaccat ttagtataaa agagctttta
    10501 gtcagagttt ctgctcttct tagaagagta gcaaaggatg attcaagtgt taagtcgagt
    10561 gagatagttt ctccaccatt tatacttgat atagacaaaa gaaaattatt taaaaatgga
    10621 aaggaaattg agcttacacc aactgaattt tcaatagtga aatatctaat atcaaatgct
    10681 aagcagtcgt taagtagaga ccagatatta gacgaagtat ggggaactaa ctatttatat
    10741 gattttaaaa tagtagatgt aaatataaga aggataagaa ataaaataga agatgaccca
    10801 tcaaaaccaa aatatataca aactatttgg ggttatggat actgttttag aaaggaagaa
    10861 taatagtgtt acattttgaa ggtctgagaa aaaaagtaat aaaaaactat tttataataa
    10921 ttattataat ggttactctg tttgaaggat tgttcatgtt ttacatacaa aactactatt
    10981 atgattctgt caaacagcta cttgaatctg aaataaaata tgctgatgaa tataatgcta
    11041 ttacaatgga aactacaagt tttgagaaaa aagtaaaaaa tatatttgat aaacaaccct
    11101 taactaaaaa ttcagaattt ggaatttcaa taatagataa agataaaaat attatattag
    11161 accaatatgg ttttaaaagt aaagaaaaag caaactatga agatgttaac aatgcattaa
    11221 aagatataaa aactaaaaat ttaacacctt atacttatag aataccagat acaggggagc
    11281 atgttatgag tatctcctta ccacttaagg taaataatat aatagaaggt gttgttagat
    11341 atacagtatc attagatgct attgataatg caatattaaa gcaagctaca tggcttatat
    11401 tagcaggaat atttatactt attatagcta tactaataag tcttaaattt gctgagactc
    11461 ttataaaacc attacgagag ctaaagaaat ttgcaaatga attggctgta ggaaattata
    11521 atataaaatt agaaaagatg aagattgtag atgatgaaat tggtgattta gcacagacat
    11581 ttgaacacat ggcacatgag attgacaaaa gtgaaaaatt aaaagaggaa tttatatcat
    11641 ctgtatctca tgaactaagg actcctctta catctataaa ggggtggagt gaaaccttag
    11701 gttatgaaag tataacaaga gaagaactag acttaggttt aggcattata caagatgaaa
    11761 cagaaagact tataaaacta gttgaagaat tattagactt ttcaaggctt tcttcagaca
    11821 ggataaaact tcatgttgat atagttgatg ttgaaggttt aattgtaggt gttgtaaatc
    11881 aacttaaagt taaagcagct gaaaaagata tatcactttt atttgaattt gaaaatgaat
    11941 ttatagagaa cattcaagga gataaaaata gattaagaca ggttctcata aatttaatac
    12001 aaaattcatt taaatttacg agtcaaggtg gttacataaa agttgtagct tctcaagatg
    12061 aagagataac aaccatatca gtagaggata atggttcagg tatagaaaag caaaatttaa
    12121 ataaagtgtt agataaattt ttccaagaag attataataa ggctggtagt ggattaggac
    12181 tagcaattag taatgaaatt gtaaaacttc atggtggaag aatgaaaata gaaagtgaaa
    12241 agaatgttgg aacaaaaata acttttaata taaaaaataa atttgctaaa caagcataaa
    12301 ttcttagaaa gaggattaag atgagaaaca taaaaatgat agtagcatac gatggttcaa
    12361 gatataaagg atatcaaaaa ttaggtgaca ataatatgac tattcaagaa aaacttgaaa
    12421 atgttttaag taaaatgact aatgaaacag tggagataat aggttctgga agaactgata
    12481 tgggagctca tgcaagagga caagtggtaa attttagaac taattgtatg gattctttgg
    12541 ataaaattca aaaatattta tatgaatatt taccagaaga tatagttgta aaaactgttg
    12601 aagaagttga tgaaagattt cattcaagat acaatgtaaa atcaaagact tatatgtata
    12661 aaattgacaa taataagtat cataatcctt ttataagaaa atatgctact catgtaagta
    12721 aaaaattaga tttagataga atgagaaaag caagtgagta tttagtagga gaacatgatt
    12781 ttactagttt tgcttcttct aaatctaaga aaaaatcaaa tgtaagagaa atttattcta
    12841 taaatataaa agaagatgat aatgttatag aaatatatgt agaaggaaat ggatttttgt
    12901 ataacatggt aaggataata gtaggtgctt tgatagatgt tggtctaaaa agaagagctc
    12961 cacaagatat taagtatatg cttgaatcaa aagatagatg tcaatcatca gatactgcac
    13021 cagctaaagg actatgtctt tggaaagtaa catattaatc aattatataa aactctcttt
    13081 aaaataaaaa taaaataaat tatatagttg acataaatgg aaaaataaac tattatatat
    13141 tttataagag agatataaaa ctttatattt aaatcctgtg agaaagagag taacttctat
    13201 gtgttttata gagagctgag gatggtggaa gcttggcaaa taagtattag tgaaaagaac
    13261 tttggaggat actaattgaa tttagtatgt tagtaggtaa tctcgcctta agagaaagag
    13321 tggataagtt tatcttatca attagagtgg taacgcggat atatttcgtc tcttggtttt
    13381 tccaagaggc gttttttaat atatttaatt attagtgttt atctggtatg ttgaacattt
    13441 actaattgat tagctaagtt tatcaataag agtggtaccg cggatatttc gcctcttagt
    13501 attttaaaat actaaggggt ttttttagta ttaaaatgat tttatgtgag tctaaattct
    13561 cagagtcatt caatattaag aatttctata aaataaaaat tataatttaa gattgggagg
    13621 aattaaaaat gaaggaaaaa gtagtattag catattcagg aggacttgac acatcaatca
    13681 taataccttg gttaaaggaa aattatgaag atatagatgt tatagctgtt tgtggaaatg
    13741 taggtcagga agataaaatg gaagatgtat atgaaaaagc tttacaaagt ggagcatcaa
    13801 aggcatatgt ggatgatata agtgaagaat ttgtaactga aactatattt aaagctgtaa
    13861 aagcagaggc taaatatgaa ggaaaatact tattaggaac ttctttagca agacctataa
    13921 tagctaaaaa attagttgaa gttgctcata aagaaggtgc aaaatatata tgccatggat
    13981 gtacaggaaa aggtaatgac caagttagat ttgaagctac aattgcagct cttgacccaa
    14041 ctataaaagt tattgcacct tggagaattt gggatataaa atcaagagaa gatgctatag
    14101 actatgctga aaaacataat ataaaggtaa cagcaactaa agctaaaata tattcagtag
    14161 atgccaacct ttggcatgtt tcaacagaag gtggagatat agagcaccta gaaaatgaac
    14221 ataaaaaaga tgtttataaa caatgtgtag acccagaaga tgcatgtgat gtagctgaat
    14281 atgtagaagt ttactttgaa aaaggtgtac ctaaaaaggt aaatggcgaa gaattatctc
    14341 cagttgcact gattcataag ttaaatgagt tgggttgtaa acatggaatt ggtgttatag
    14401 atatagttga gaatagactt gttggtatga aatcaagagg tatatatgag actccaggag
    14461 gaacaatcct ttatgaagca cataatattt tagaaagtgc tactttagac aaagatacat
    14521 tacatttcaa acaaatggta tcttataaat acggagaact tatttacaat ggactatggt
    14581 attgtaaatt aagagaatct atagatgcat ttatggaaca aactcaagat aatgttacag
    14641 gaacagtaaa agttaaactt tacaaaggaa atataaaacc tgctggaata tttactgaaa
    14701 atgctcttta tgatgaagga atatcttctt ttggaaatag tgaactttat gaccacaaag
    14761 atgcagaagg atttattaat ttattcactt taccattaaa aatcagagct atgaaagctg
    14821 gaaaataata aaataaatta tttaagagtg tataaaaacg atttttaggt ttttatatgc
    14881 tcttttttta tttgaattta ttaaattata cagtaaatag tataaaaaaa tatattatag
    14941 ttccaaatgg cttaataatg tgaaaatata agacctaaaa tataaatttt ataaatacaa
    15001 tagttattga ctctctcatt gtaagagggt ttatactgaa tctaatgaaa atttatagtg
    15061 aggagataga ttatatgaga aaaataattg aaattaaaca tcattcacat gattatgagt
    15121 gtatgtggaa tggtatagaa gatttatata taaataaaac cggtgaatac cttccaaacg
    15181 attttttctt tttactgtca ggttttggtt cattttgtta tatgaaaaca aataaagctg
    15241 atttgaaaag aatggtagct ttgggagatg gaaggacaaa acagatgtat aaatttttag
    15301 caccaattgt tggatttgaa tacaaacatc atgagtttaa aaagtatgag caagcattaa
    15361 agaaagcaaa atctgaaatt gatgatggat atccagttat tttaggagct ttagatatgt
    15421 attatctacc atattataag aagctatatc ataaagaaca tattcctttt cattatatat
    15481 taatggtagg atatgatgat gaaggaatat atctgtatga ttgtggtaga acagaattat
    15541 tacatctttc ttatgataaa ttacgtgaaa gcatgaattg caattcccct ggattaagca
    15601 aagaaaatac tatttgcaca ataagaatga atacttcaaa gaataaatat aacattgcta
    15661 gagaagctat tgctataaag cgtgatatgt ttttaaatcc acctaaagga tttttgggat
    15721 ataaaggttt tgaaaagttt attaaagagt tgccacagtg gaacactgaa ttggataagg
    15781 atgattatga taagattttg accaatatgg ttatgttttt tggaacagta ccaacaattc
    15841 caaatgcaat caaaggaatt aaagaaccag atactgttca atttaaaggt ggatttgata
    15901 aaatgagtag aatgctcaac agtattgggg aggaatacaa aaacaactct tttatacaag
    15961 tagctgatat ttttgataaa actgctatta taattgagaa aatatccaat attataatag
    16021 attacttaac tcaaacatgt gatgatacag aacaacttcc gttattgttc tctgaggtct
    16081 tagagcatat gaaaacagga tatttaatct tggatgtata aaaaatatat tgttgaccct
    16141 aacattacgt aatagtgtaa tatgaaaata tagcttaaat atattagtag gtattgatgg
    16201 ctgtaaaatt aaaatttgag gtgtggatat gagaacagtg aaacaagttt ctgatttaac
    16261 tggaataagt gtgagggcat tacattatta tgatgaaata ggtcttttaa aaccaaataa
    16321 aattacagat gcaggatata gactttatga tgatgaatct attaaaactc ttcaacaaat
    16381 tttatttttt aaggagattg atattccttt aagagaggtt aaggaaataa tgtcaagcca
    16441 atattttgat aaggttgaag ctctaaaaaa tcaaaaaaaa ttacttattt taaaacgaaa
    16501 gagactggat gaattgatag agcttataaa tcaaactcta agaggagaag gaaatattga
    16561 ttttaaagag tttgatatga gcgagtattt taatgtttta gaagaattta aaagagagca
    16621 taggaataag gtaattaaaa tttatggtag tgtagaaaaa tacaatgaat atatagaaag
    16681 agttaagtct aatgaagaga aaattgctaa aatggctata aaacaatatg gaactattga
    16741 gaagtttgct aaagctataa aaacaaattt tagcagtgat atattgaatt taggagaaaa
    16801 atttgataga tataaaaatg attgtttaaa agaaaaacat ccaaaattaa aagaattata
    16861 tagaaagctt gtagaagatt taagtaggaa tccttcttca acagaccttc aagaaatagc
    16921 aaaagcgata acagatatat ctaaaaaaga ttatgaaatt ttctctatgg atactggtga
    16981 tgacaactgg tattatatgg ttcaaaatta tctagtaaat cctatgtgga tagaagaagt
    17041 agataaaaag tatggaagtg gagcaggaaa gtttattgga caagtactaa agacttatct
    17101 aagagataga aaaccaaaga taaatacatt gtatgaaaaa cttgtagaag atttgagtag
    17161 agattgttct tcaagggaag ttcaaagcat tgttgaagaa atagacaatg aaatgaaaag
    17221 aagtaatgaa ttttataaaa tagacaatgg tgaaagatac tttgattata tgtcagagct
    17281 ttatttacaa gattctaact atataaaagt aactgataag aattatggtg atggtgcatc
    17341 taagtttatt ggagaagctt ttaagattta ttttgataat aataattgtt gaaaatttgg
    17401 aagtaaaaaa tatgtaagtt ttataaaagc acaaaaataa ggatgtagat tagctccatc
    17461 tatatcctta tttaaagtta ttaaaatata tagtttacat caaacttaac actttcaata
    17521 atatttccca aatatgtttt tatactttca acttcttcta aagattttaa tttattttca
    17581 gtataaattg ctttgtccag aatattaaac cttaatccta tttcatcaaa gtactcatga
    17641 tttaaaactt tgtaccaatg gtttttttct ttattccaac tcgtagatat tgaatcagct
    17701 tctttttgag ctttttccca atcttcattt tctatagatt tttctaaaac agatatattg
    17761 tctttatatc cttctgtaaa atcatatagt ttattgttaa cgtaaaatcc aaataataca
    17821 aataagatag tccatagtat aacaaatgtt gtagatttca atattaatca ccctttttat
    17881 tttgaataaa tattttgtca ttttcatcta aaacacaaat aagaacatct ttaaaagttt
    17941 ctatgtgatt agattttaaa atacccatta accagttagt atctttttga gctatgtcta
    18001 gatttttatt tattattcta ccatccagta taagtgggat aggtaaatga ttaaaagctc
    18061 ttggaggagt actattgtag cttgatgctg gtacaacact tagattacca tcagtctcca
    18121 aaatagcata ttgtacatct tttaggttaa agtatccttg aatcctaagt tgttctaata
    18181 attcatctat actaattctt tcttttttaa gctctttata tacaattttt cctttatcta
    18241 ttaatacaga aggtttacct gataaaaatg aacttaattt tctgcttttt aatgataaga
    18301 atgaaattag agtctgcatg attacaagtc cagatattgc tgcaacacca tttatgattg
    18361 gtatattatt attttccatt gggacagatg caacttctgc aattaatagt gttataacta
    18421 aatcaaaaca gttcatttca gctatttcac cttttcccat gactctaagt gcaattagca
    18481 ctgttatgta taatataata cttcttatta atactactgt catatctatc aattactcct
    18541 ttaaaaaata tttacactta gttagtatat cctatataaa aaatttaatt caaagtataa
    18601 aaaaccttaa ataattggat tcaactattt aaggtttttt tgataatttt aatattaaat
    18661 tttttatgga ttttctgtta cttttattta ttttaataac agattcaaca aaactctttt
    18721 tatctcctaa ttttaagata aaatcaggat attcttcaaa ttcattgagt tttattaatt
    18781 tgaaagaaga agatagattt ttaaagttaa taattttaga gttattcact aatacacttc
    18841 tttttttaaa ataaatttct tttttactta tatttagtat agttaatatt tgtttaagat
    18901 atattattat atcttctgga gttctatcaa atttgagttc aaaattttca tcttcaaagt
    18961 agggaatata atataaaggt gttttgagag caggtaattt attatataat tcttcaagaa
    19021 tatcgatatt tttaggggag ttaaagaaaa agcatttaca tacattcatt aaatccacag
    19081 catctgaaag tgagttatgt ttaatatctc cttctacaga aaaaattttt ttcatattca
    19141 gtaaagatat ttgcttagat aagattctat tattgtatgt aatatgttga gatataaaag
    19201 gttgaatgtc aatcatcata ttaaataact ctaagcattt acctgtatat ttatacactc
    19261 taaatgaagt tagaaggcca gttatatcaa aatcacccca attatatatt gtaacatcat
    19321 caaatttaca aaaccattta taaaaatcat ccataacata taaaagatta ggagcattat
    19381 tgatatctag ttggttcaat ttggttaaat ttttacattt atttgaaagt attttattgt
    19441 attttggctt aactaaagag taataattat ctattgtatt aaattcgtta tcaactaaaa
    19501 ctccaccaat agaaattaat tccgcaccat tatattcttt tggtttattt ttattatcat
    19561 ctagtatatt aaattcaaag tctataaata acttgtattt cataaatcta aagcacatcc
    19621 tcaggtaaat tatttattga gcctaagcat cataagaatc aatagatata atgactctaa
    19681 acatcaaagg ttcattacaa cttttattta tttcataatt taattcaaat aaatctttta
    19741 agatataatt tataagtatt ctttcccaac ccatttttaa attaacttta gagtctatta
    19801 atatattttc tatcttttta aatatttcta tatttgctgg tattatactg agctcaatat
    19861 tacttttaaa cgggttagac tgtaaatctg ttataagtat attttttaat tcctctgtaa
    19921 gttcagaaat taaactatca cctatgtatt tttttatgat ttcctctcta atttctttta
    19981 aatttagcaa tattagtacc tgctacttac atagtagctt ttcctttcct tttaatacta
    20041 ttattcattt ttgaatctta tttagatata ttttaagatt aaaagtcttt agctatataa
    20101 ttttttaccc atttttaact tatattaaca tattacaggc ttatatctcc accctgttta
    20161 cattgaggat ggagtatatt gcctattttg ataaaaatga taggttgcta tttgcctatt
    20221 cctttttctg ataatttttc taacatatct ttggtcattt tatctaaatc ataactaaac
    20281 ttaaagttcc attcttttac tgcactacta ctgtctagag agttaggcca agaatcagct
    20341 atactttgtc ttacagggtc tacatcatat tctatcacaa aatcaggaat atattttttt
    20401 atagaatcag ctatttcttc aggagaaaag ctcatagcag taatattaaa agcatttcta
    20461 tggatgagtt tatcagctgg agcttcgatt aaatccacta ttgattgaag tgcatcaggc
    20521 atatacatca tatccatttt tgtaccttct gctatgtaag atttatatct cttatttttt
    20581 aatgcctcat aatatatatc aactgcataa tctgtagttc ctccaccagg aggcgtaaca
    20641 taagaaataa gacctggaaa tctaacacct ctagtgtcta ctccaaattt tgagtgataa
    20701 taatcacata gtaattctcc agaaacttta gtcacaccat acatagtatt aggtctttgt
    20761 aatgtatctt gaggagtcat attttttgga gaattttcac caaaagcagc aattgaacta
    20821 ggtgtaaaaa atttacaatc caattctttg gctatttcaa gaccatttat taatccattc
    20881 atatttaagt tccaagcttc tagaggttta ctttcagcaa ctgcagaaag taaagatgct
    20941 agatgaatta tagtatctac atcatatttt tttgcaattt caaaaaaacg attcttatct
    21001 gtaacatcta atatctcaaa tataccagat tcacatactg gattaccttc ttttactctc
    21061 cttgaactag cgattacatt ttgttctcca tactcatttc taagctttat tgttaattct
    21121 gaacctattt gtcctaatgc acctgttata agtatttttt tcaattttac aacctcctaa
    21181 gggtttaatt tatgtagtac tacttatttt tattttagca aatcttttgt ttaaatacaa
    21241 agaattaaag ataataatat acgacttaat tgaatgaagt gcagtaagtg cgtaaaaatt
    21301 taaagctaat atataatggt ctattgtata acaataaaag agtagattaa attattatat
    21361 atagattgtg tgactaagtc acagaaaata gagctaattt aatatataat aaaagcatgt
    21421 caagtaaagc tttaaattaa agtaacttta tataaaaagt aatataaatt gataaaatta
    21481 taaatttatt tatagtgaat ataggttgga ttttatagat acaaaaatag tatatatgat
    21541 atgtagacca ttagcatgtt taaagtagca aaacatataa aataaaattt gaaaggaagt
    21601 ataaaaatga caagaccatc aatatatcat agccaaggat acacttgtgc agaggcatta
    21661 ataaagtctt ataatgagga acacaacaca gatataccaa tctcaatagg aagtggtatg
    21721 ggggttggaa tgaatgttgg aagtgtttgt ggagctgtaa atgctgctgt tgttataata
    21781 gggtatttaa atgggcgaaa tagtaattta gatgaaaata tagcaaggaa gtattcaaga
    21841 gagctattga atagagtaag aaataaatac agctcagaaa tatgtgtatc tcttaaaaaa
    21901 aataaagtaa cttgtgaaga aataataaat ttcacatatg atgctctaaa tgatatgcta
    21961 aaaaatcaaa aataattcta agaaacaagt attttacatt cgatagaata cttgtttttt
    22021 tatgatattt ttaataagta ttagtttatt ataattttta aatcaatttt attatatttt
    22081 aattgaaata atctactttg aaaattacta actgacaaaa gaatatgatt atagtataga
    22141 aaaaatgtaa attattt
//
LOCUS       DAMJWU010000056        22124 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMJWU010000056 DAMJWU010000000
VERSION     DAMJWU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.056"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(141..1412)
                     /gene="serS"
                     /locus_tag="PC460_002303"
     CDS             complement(141..1412)
                     /gene="serS"
                     /locus_tag="PC460_002303"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2601184.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKNFNLDEVVKLDDERRKTLQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            complement(2228..3832)
                     /locus_tag="PC460_002304"
     CDS             complement(2228..3832)
                     /locus_tag="PC460_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2601185.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            4218..5144
                     /locus_tag="PC460_002305"
     CDS             4218..5144
                     /locus_tag="PC460_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2601186.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNTYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(5264..6262)
                     /locus_tag="PC460_002306"
     CDS             complement(5264..6262)
                     /locus_tag="PC460_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF2601187.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            6366..6803
                     /locus_tag="PC460_002307"
     CDS             6366..6803
                     /locus_tag="PC460_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2601188.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            6889..7476
                     /locus_tag="PC460_002308"
     CDS             6889..7476
                     /locus_tag="PC460_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2601189.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(7776..8396)
                     /locus_tag="PC460_002309"
     CDS             complement(7776..8396)
                     /locus_tag="PC460_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HDF2601190.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(8404..9171)
                     /locus_tag="PC460_002310"
     CDS             complement(8404..9171)
                     /locus_tag="PC460_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDF2601191.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(9212..10033)
                     /locus_tag="PC460_002311"
     CDS             complement(9212..10033)
                     /locus_tag="PC460_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601192.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(10392..12302)
                     /gene="phnW"
                     /locus_tag="PC460_002312"
     CDS             complement(10392..12302)
                     /gene="phnW"
                     /locus_tag="PC460_002312"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF2601193.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(12581..13366)
                     /locus_tag="PC460_002313"
     CDS             complement(12581..13366)
                     /locus_tag="PC460_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2601194.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDAMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKAYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(13583..13813)
                     /gene="dltC"
                     /locus_tag="PC460_002314"
     CDS             complement(13583..13813)
                     /gene="dltC"
                     /locus_tag="PC460_002314"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF2601195.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(13832..14980)
                     /gene="dltB"
                     /locus_tag="PC460_002315"
     CDS             complement(13832..14980)
                     /gene="dltB"
                     /locus_tag="PC460_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF2601196.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(14980..16494)
                     /locus_tag="PC460_002316"
     CDS             complement(14980..16494)
                     /locus_tag="PC460_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF2601197.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(16615..17805)
                     /gene="dltD"
                     /locus_tag="PC460_002317"
     CDS             complement(16615..17805)
                     /gene="dltD"
                     /locus_tag="PC460_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF2601198.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(18093..19112)
                     /locus_tag="PC460_002318"
     CDS             complement(18093..19112)
                     /locus_tag="PC460_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2601199.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(19288..19827)
                     /locus_tag="PC460_002319"
     CDS             complement(19288..19827)
                     /locus_tag="PC460_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2601200.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(19928..20608)
                     /locus_tag="PC460_002320"
     CDS             complement(19928..20608)
                     /locus_tag="PC460_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2601201.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(20637..21926)
                     /locus_tag="PC460_002321"
     CDS             complement(20637..21926)
                     /locus_tag="PC460_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2601202.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         7233 a         3817 c         2268 g         8806 t
ORIGIN      
        1 tttttattac ttctatatta ttgtatcact tttatattaa ctattttttt attacttcta
       61 tattattgta tcacttttat attaattatt ttttattacc tctatattat tgtattactt
      121 aaaaatacat aatttaactc ttatttaata acagaaactc ccatatatgg tcttaaaact
      181 tcaggaacaa ccacagaacc atcttcttgt tgataatttt ctaatatagc tgcaacactt
      241 cttccaatag ctaacccaga gccatttaat gtgtgtacat attcagcttt agaatcttta
      301 tctcttttaa atcttattcc tgctcttcta gcttggaaat cttcgcagtt tgaacaagat
      361 gaaatctcaa catacctatt gtaacttggc atccaaactt ctacatcata tttaaaagaa
      421 gccgtaaatc ctaaatcacc tgtacatatc tttactactc tataaggcag attcaataat
      481 tgtagcatag tttccgcatt gttagtaagc ttttctaact cattgtaaga ttcttctggt
      541 gcaactattt taaccatttc tactttgtta aactgatgct gtcttactaa acctcttgta
      601 tctcttccag cagaaccagc ttctgaccta aaacatggag tataagctgt ataatttatc
      661 ggtaattgtt caaatttaag tatttcatta gcatgtatat tagttaaagg aacttctgat
      721 gttggaatca taaaatagtc cataccttct attttaaaca tatcttcttc aaacttaggt
      781 aactgtcctg tacctaaaaa actatttcta tttgccataa atggtggaat aacttctgta
      841 tatccattta cttttgtatt cgtgtctaag taaaaattta ataatgctct ctctaatcta
      901 gcacctaatc ctttgtaaag agtaaatctt gaaccagtta ttttactagc tgtttcaaaa
      961 tcaagtatac ctaattctgt tccaatttcc caatgagcct tagattcaaa atcaaaggta
     1021 gtagtctttc cccaagttct tacttcaaca ttatcttcat cagtttcacc ttgtggtact
     1081 tctggatgtg gtacatttgg tattctcatt aataggtatt ccatcttatc ttctacttct
     1141 ttaagatttt ggtctattac cttaatttta tcagataact cttttaattt aatcttttca
     1201 ttttctacat ctttaccttc ttttataaga tttggtatat ttttagatgc tgtatttaat
     1261 tcatttttca taacttcaac ttcttgaaga gtctttcttc tttcatcatc taacttaact
     1321 acttcatcaa ggttaaagtt tttttcgccc cttctttcca ttgctttttt tatacaatct
     1381 aaattttctc ttattctttt tatatcaagc attttttcct cctcagtacc taagattgga
     1441 tttatctctt aatctcaaat aacttagata atatacttta tgcttgaggt taagaaacct
     1501 ttttctttat ccgaacctgt tatttttatt ttatttatat tttactcact tttttgccca
     1561 ttgtcaaatt atatttaatt aaataaatga aatttaattt taggagaata ttgctagatt
     1621 taacttcatt ataattatat ctattttgct aatcaattat ctattttgac tttttcttta
     1681 tcaatcttgt atagttatta acttttatat aaattttttt atattataac atagtcctat
     1741 atatctatta tttggataga attatttttt aaattagaat tattttgatg aatgattcaa
     1801 gtaataggta tgaaattgaa tcttttagaa ctgcttacaa aaaatactca aagaaaaata
     1861 gtaattttct cagattcaca atatatttta tatgaaatgt tataagttaa tctatttaat
     1921 aaatttaaat ttgctattat agatttagtt ccaaagtctt actgcaaagt aatatacata
     1981 gttttttaat aaactaataa gtgcaattaa taactactta attgtttaat atagtacata
     2041 gataacttta ataaatctac agttatttaa aaaggtagaa tcaataaaat tatacttata
     2101 aatcttcaat acaataatta aaatttaata aaaatataat tgttgattta actgtaatct
     2161 tatttaattc tacctaactt attatataaa actatttttt atagattata aaatatctaa
     2221 ctttcatcta cttcaaagaa tacaatactt ctccttctct tatagtctgc aatactttaa
     2281 tatcttttat tttcataggg tcgattgtaa gtgggttttc atctaatatt atcaaattag
     2341 ctaatttccc ttctttaatt gaaccttttt tatcttcttc aaaatattga tatgctgcat
     2401 ttatagtaac accctttaaa gcatctaatg ggcttatctt ttcattttct cctatttgta
     2461 tacctttttt tgtaattcta ttgacagcac accatactgt ttccagcata ttaggtgcta
     2521 ttactggtgt atcctgatga agagtgtaaa ttaatccctt ttctatagtt gttttaagtg
     2581 ggctaatctt aaacgctcta tcttctccaa ggtttttgat atgaatatca ccccaataat
     2641 atgtgtgagc tacaaaatat gaaggtatca tatttattac tttcatatca tcaatctggt
     2701 ctgctcttac tgtttgagca tgtatcataa ctggtcttat attattttca ctactttgct
     2761 cttttaagtt taaaactttc tcaaacgcat ctatcaactg gtctgctgct gcatctccat
     2821 tacaatgagt taagagttgc atcttttctt ttagtgaaat atctataaac ttttctactt
     2881 cttcatcttt gtatatagga tatccacaat aaccatcatc tgaattttca tatggtctac
     2941 tcaaccatgc tgtcttacct tgtggagaac catctaagaa aatcttataa cctcctattt
     3001 taaatctatt tatatatttt tttataaact ccctattatt atctgctaca gacttacttt
     3061 ttttcaaatt tacatatcct acaacatcca tttttaattt attttgattt gccattgctt
     3121 ttaaaatgtt aaattgattt tcttccatta aaccttcttg agctgttgta ataccatagc
     3181 ttagatatat gttctgtgct ttttcaattg aattaaaaat ctcatttgat gatggttgtt
     3241 ttattttaga tgctacatta aaaaatgcat tttcttctaa atatccattt ggttcttcac
     3301 tgccttctac tctacctata tgtccacctt ctgggtcacg tgtttcattg gtaacaccaa
     3361 gctgttttaa ccccaatgta tttgcaactc ccatatgacc agaagcatgt gaaattagta
     3421 ttggattttc tgaagatgca ctatcgagta cactcttatc tggatgctta ttttcttcta
     3481 aaaaattatt atcatatgaa aatccaatta tccagtctcc tttttttata ttattcgact
     3541 ctttgaaatc ctgtattttt tttactatgt ctttaaaact ggttgcatct tctaaaggaa
     3601 ctagctttag agtagtagca aaagctatca agtgactatg tgaatctata aaagatggca
     3661 tcaatgtctt accttgtaaa tctataattt ctgtatcttt atttttcaaa gcaaatactt
     3721 cttcttttgt tcctatcttt tttattattt tgtcttttat aagaatagca tctccacata
     3781 ttgaatcttc cattgtgatg atatttccat tgtaataaat ttttgctttc ataaaattct
     3841 ccttacgttg tctactttaa taaatttttt tcattatttt ctgcttaatt atattatctt
     3901 ctttataaac tttttcttta gagtttaatc atataaaagt tttattttta attattgttt
     3961 gccaagaagt aaatactatt cattgtaaat aagacaaatt aataggcttt atactaattg
     4021 aaaaaatatt ataagttgtt aaatttaaat atctaaactc aattatatct gtacaattgt
     4081 aattttttaa atcatgatgt attattttaa atatacagtt acattattat tcacaatata
     4141 agtatcttag ttctcaaata acatctcatg taatctattc aatgcaaaaa atttaaatat
     4201 tttttaggag gttttttatg agcaaggaaa ataaaaatgt aagaattgct gttgtccagg
     4261 cgtcaccagt cataatggat ttagaaaaaa ctgttgaaaa agcattagga ttaattaaag
     4321 aagctggtag aaaaggggca aatatcgtag ttttccctga agcattcata cctgcatatc
     4381 caagaggtct ttcatttgga tttgttgttg gaagtagaac aatggaaggt agagaagatt
     4441 ggaaaagata ctatgataat tctgtaccag ttcctagtgc tacaacagat ttattaggaa
     4501 aagcagcaca agaagcagga gtatatctat caatgggtat aacagaaaga gatggtaatg
     4561 atataaattg taccctttat tgcacaaact tatttttctc tccagaaggt aagctaattg
     4621 gtaaacacag aaaacttaaa cctacaggaa cagaaagatg tatttgggga gaaggagatg
     4681 gaagtactct tacagtagtt gatactccat atggaaaaat gggttcgttg atttgttggg
     4741 aaaattatat gccattagct agaacagctc tttatgctaa gggtgtaaaa ctttacatag
     4801 cccctactgc agattcaaga gaagagtggc aagcaactat gaaacatatt gctttagaag
     4861 gaagatgttt tgtaattgga tgcaatcaat atgttgaaaa aaatacgtac ccaactgacc
     4921 ttaactacta taaggagcta gatgcagaac cagaaattat gtgtccagga ggaagttgta
     4981 tagttgaccc ttttggaaaa tatgtagcag gtcctatttt caataaagaa gaaatgctaa
     5041 tagctgattt agacctagaa aaaatagtac ttagtagact agattttgat tcagaaggtc
     5101 actattcaag accagatgta tttgaattaa ttgttcatga ataatctttt tacacaaaca
     5161 tctacataaa tctttttaca taaaaaaatg agatactcat aagtaaattt caagtatctc
     5221 atttttgctt aaaacctata atcctaatta taaatttatt cacctatatt ttttctaaat
     5281 caactactgt tcttccaaca tgttttcctg ccattattga atctatacta tcatttaact
     5341 cttccaatga tactacattt accatatcat ctaaatcatc taatttccat tcatttgata
     5401 ataaatccca tatttttaat cttaagttca ttgggcaatc cacagaatct ataccatata
     5461 aagtaattcc ctttaagata aatggatata ctgaacttgt aaaatcaact cctcctgcat
     5521 taccacaagt tgttactgct ccacaataat tagttgtctt tattgctgtt gctagagtat
     5581 ttcctcctac tgtatctatt actccagccc atcttggtct aagcagaggt cttcctgagt
     5641 tatcatccac ttctgaccta tgtataacat ctttaacacc taagtttagt aatacttctt
     5701 tttgttcttc taattttcct gttgaaacta caactgaata acctaattta atcaatattt
     5761 tagttgcaat tacagcaact cctccagtac caccagtgac taatacatct cccatattct
     5821 tgtctacaga atctataaat ttatacacag ataaagctgc tgtaaaccct gctgtaccat
     5881 aatccattgc ttcttttagt gaaagatttt taggaagttt aactacccaa ctagaaggaa
     5941 ctcttatgta ttctgaaaaa ccaccagaag tattcattcc caagtcataa cctgttacta
     6001 aaacttcttc tccaatttta aaatcatcac aactactaga ttctactata ccagatgcat
     6061 caattcctgg tgtatgagga tactttctcg taacaccctt attaccattt gcagaaagtg
     6121 catccttgta atttaaagaa gaatatttta cttttattaa aacttcgcct tctggcaatg
     6181 tatccacttc tctttcaact atatttcttc taaaatttcc ttcttcttct gtaatatata
     6241 gtgatttaaa ttttttattc ataaatttcc tcccaataca tttagtttta tgttaaacta
     6301 attatagttt ataaatagtt ttatgtcaaa ctaatttaaa tagattaaag attggagaat
     6361 aatttatgga agataaatat attgtacatt tcataagtaa aacaaaggct agtatgatta
     6421 aattcattga aaataaactc agtaaaaatg gtttaggtga gttaatacct actcatggaa
     6481 atattttaac tgcactttat gaaaataatg gaatacttac aatgaaagaa attgcagcta
     6541 aaataggaaa agataaatcc actgttacag tgttggttaa taaacttata aacctaggat
     6601 atttagaaag acaaaaatgt acaaatgaca aacgtatcac gtatataaag cttacaaaaa
     6661 aggcactgct tatagaagat acttttaata gcatctcaac acaagttaaa gagacggcat
     6721 atcataatat tacagaaaat gagaaacaag aatttcttag aattttaaaa aaaattaatg
     6781 ataattttaa aaatgctgac taaattgagc ataattatat ctcctattgg aataataaat
     6841 aaaaagaatc aaatattgta aaaatttatt gtgatagaga ggtagaatat gaatttaaaa
     6901 gatagtaaaa ctaaagaaaa tttattaaga gcttttgcag gtgagagtca agctagaaat
     6961 agatacaata tttctgcatc tgtagcaaaa aaagaaggat tatatataat agagtcttta
     7021 tttaattaca ctgctgacca agaaaaatca catgcaaaac aattcttttc taaattaaaa
     7081 gaatttgctg gtgaaaatat aagtatatca ggagcatacc ctgtagattt atatgacaag
     7141 acagcacaac accttaaagc tgcacaacat aatgagcttc aagagtggga tgatgtatat
     7201 aaaaactttg gaaaaacagc acgtgaagaa ggatttgaat ctatagctaa gttatttgaa
     7261 aatatagctg aaatagaaaa agttcatggt gatagatttg gtagatatgc tactgattta
     7321 gaagaaggta ctttattcag aagaaatgaa gatatacaat ggatatgtac acattgtggc
     7381 catatacata taggtaaaga agctccaaaa gcttgtcctg tatgtcttca tccacaagga
     7441 tatttcttag attttaaaaa ctcattattt gaataaatta aggcaataat tctatttaaa
     7501 caatttacta tatttatatc aagtattttt ataaatactg tattatctta ataacaataa
     7561 aatgaaccat tgatattact ataagtagaa aatgtagttt aaatacaatt tatagtaatt
     7621 catcatggtt cattttattg tttactaatc cctataacct acttaaatct atctttaact
     7681 tttacatact cacataaaat cttatcttta aactaaatat ttaaataaag tgtattttaa
     7741 gtatattatt ataatacatt atataagata atacactatg ataactttat acttatatca
     7801 tttatttctt ttgtcaagtc aatctcaata atcttaacta catctttttc aaactttact
     7861 tttttataat ccagacatcc tactacgaat ggaactccaa actttctaaa caaataaatc
     7921 tttccacttt cattgtatat tgatgtaaaa ttttcttcta ttatattcac tatcaatttt
     7981 aactcttcaa aagttattat agatttaact aaaaacttct ctattatgct caatataagc
     8041 agttcttttt tacctatgct ttgctctttt ttatataaat tttgaaatat ttctttggta
     8101 taattacata taacttcttt actgagaata aaatcaatgt caaattcttt attaatatcc
     8161 tttttcataa acatatttgc aatatcatca agagaagtct cttctttcat agacaatatt
     8221 aactctattc tttctagtat tttatctctt ggaaagaaag tctcttgacc tgtaaaagct
     8281 gattttttta taaaccattc ctctggtatt atgttttttc ttttccatct atataattga
     8341 ccataagata tattagtttt gctcaataaa tctttcttag atattaattc ttccataact
     8401 tttctagccc acctttatac tgttttttac agtagaatta tctgaaactg ataaattatc
     8461 actgtattca atatttccaa catcacaatt atctactaga gatatgcttt tacctcgtat
     8521 atcttttatt gatgcatctt ctaattcaat agtatctccc tctatcaaat tacatttaaa
     8581 ctttcctgaa aaaatattaa tgattttaat taatgaaaaa gcatctttgc ttcttcctat
     8641 ttttatatta gttgccccta tctctttaca ataagaacct cctcctgaat aaacatatat
     8701 attttctcca tttaaaagac catcacattt tattgctcca tataccttta tatcttcaca
     8761 ttctatattt gatttatttc tcatttcgcc atagattttt atatcttgtg catacatatt
     8821 ttcatttaca cttaattgtc ctcttattgt aacattatta catttaagtt tgccatccac
     8881 tttctgttga gcagttataa ctaagtcatc tatttctgca tctccctcta tattaagttg
     8941 accattcact cttacacgct ctgctttaag attgcctctt ataatagttt ttccgttaac
     9001 aacaaaattt tttatattga tgtcaccttc aaattttgca tctccaagaa cttttacttc
     9061 gttactttta acatttcctt ttgatttaga gcttcccacg atttttatat tttcatactc
     9121 accactactt ataaagcctt ctccaagtat ttttatagac cctttttcca tattattacc
     9181 tcattttaaa ttttattttt aaacctactt attacttatc cttctttaat tttatttttt
     9241 cacttatatt ctttatttca ccaactattg aatcttcatc aatctctata ctttcactgt
     9301 actccaaact ttctacataa catcccttgc ctattttaat attttttcct cttacaactt
     9361 tgacttcact attttctaag cttatttcat ctgcttctat aatatttgca aacaatttac
     9421 tttctgtata ctttctaatt gataatagac ctaaacttat caaatttgaa gacacttttt
     9481 caaagttgat atgctttttt tctagcatac gaaatttttt gcaataatct ccatgcccct
     9541 tttttatatt tatactactt gctccaactt cattaaaatt agaagttcca tttaaactaa
     9601 tattaacttc ttcacaattc aaaaatccat tacactccaa gattccactt aaattgatat
     9661 tttcacaatc tatattctca tttgctttta aatatccttc tccatgaata atatcagatt
     9721 ttatacttcc ttctacatta ataattccac tagtatcaat ttcaacagaa ttaatatttc
     9781 ctgtacatct aagaagtccc gatatctcaa cttttgatgc cagtatgttt ccttctactt
     9841 tacttaaacc agatgtaaaa aattcattac attctgttgt cccaaaaact tttgctgaac
     9901 cagaagaatc aaatttacta cattttacac ttccttctat tttgcctgtt cctgatatat
     9961 taacattatc aaaaattcca cctttacatt ttccaacacc atcaaatctt agtgaactta
    10021 attcttcatt cataatgata tcctttcttt accgtatttt atctttaaac ctaatataac
    10081 aaaacattgt tacgttgtca agtatgataa taatacttaa taaagcaact atcatttatt
    10141 ttctataaat aggcataaaa ataataggga aatgctttat ttaaactata aatctaattt
    10201 cttagattgt ggtttaaata aaatatatcc ctattactca atctttatat attcattgta
    10261 taaacttcta aaatataagt ctttaaattc tactgtcaat actttttata cactgtttgt
    10321 gctatcatct cacacaccta aaggagtggg ctttccacac tattctgtaa cctacaagac
    10381 actatcttct tctatctatt gataaatttt tctattacat cagctagtct ctccatatca
    10441 gttggataaa cttcacctat gctacctatt ctaaatgtat ctatatcagt caatttacct
    10501 ggatagataa caaatccatt atcttttaaa taagtataaa attccataaa ttcaaacttt
    10561 gcatttttag gatataagaa agttgttatt acaggtgatt gagcattatc attcaccaaa
    10621 gtatcaaatc ctaaagattt taatcttgaa gctattgtaa actgattttc tttatatcta
    10681 gcatatcttt tttcaacaga accttcttct tccaattcta ataaagcttg ataaaaagct
    10741 cttacaacat gtgtaggtga ggtaaatctc catttaccat tatttttctc catagtctcc
    10801 cattgcgcat acacatcaag agataatgat ttagctatac ctttacattt agataatttc
    10861 tctttattag ctattataaa accaaatcca ggtactcctt gtatacattt gttagaacta
    10921 ctaactaaaa aatctatatt aaaatcttca acatcaatct caattccccc aaaactactc
    10981 atagcatcaa ctatataaat tttgttatat tcttttgcaa gtttccctac ctcttgtata
    11041 ggatttaatc tacctgtagt agtttcacag tgaaccattg atatatgtgt tatatccttg
    11101 ttttctttta ataaattttc tactacattt aaatccactg cttctatatc tttaaaagtg
    11161 cagtcaacaa attgtatgtt aagataatta catatatctt tcattctctt tccgtatgct
    11221 ccatttgcaa ttacaagaag tttaccatct tttgaaatag tacttcctat tatagcttct
    11281 actgaaaaag tcccacttcc ttgcattaat acacttgtgt atttatctgt attttgggtt
    11341 gctagagata ctaatcttct tctaacatct tgtactaggt tattatattc aacatcccaa
    11401 gtacaccaat cttttaacat agatgctctt actgatttag tagtagatag tggtcctggt
    11461 gtaagtaata tgtaatcatt ttctggtaca aaatctgatt taatagtttc attctctatt
    11521 tttattagta tatcttctaa ctcagccata gtttctatta caaaatgtgc tcctgcctct
    11581 ttaaatttct ttgaaactat actcatctta gcttttaatt cttccttatc catgttctct
    11641 acttcttctt gtgtaagccc taactcactt gaacctttta ttacagcaac actccacatt
    11701 cctgcattta cgccttcttt tacatcactt attgtatctc ctacttttac catagaactc
    11761 ataggagaca cccctaaagc ttcagcattt ttatagcaca tccaaggata tggtcttcct
    11821 tgagatactt ctgatggtgt tacaagaaaa tcgggtgagt atcctttctt tgctgcattt
    11881 ggttcaacta tgttcatcat ctctcttgta taacccgttg tagaacctat ttttaatcca
    11941 ttttttctca acttttcaat agtttcaaca acatgaggaa ttggagtcgt atactcttct
    12001 aatgtctcaa ataacattgg ctcaaattca gcataaagtt catttacatc atcttcagtt
    12061 ggcactttac caaacttatc tgaccaaata ttctttattc tatccatttc acacatttct
    12121 cttatatggt ctatttttaa ttttcccata ggttttcttg cttcttccat tgttacatct
    12181 atacctcttc ttttaaatat ctctataaat acattaagag gtgcaaaaca tccataatct
    12241 actgttgttc cagcccaatc gaatactact gcttttattt tttctccata tatctttttc
    12301 atatttatag cctccataga ttctttttag aatttttgtg tttttaattg tttttacttt
    12361 taaagtataa ctttcatatg ttaattatac atcatattta ggttaaatat atattaaatt
    12421 tctgttttta gttgtattta gttgttttta ttttgtatca tattcccata aaatcctaag
    12481 ttacaatcta aaatttactt tctaatctta atctatgaac tgactatatt ttatgaattt
    12541 agttgtattg agcatagttg tattatataa aactaagggt ttattttgct tctatccatg
    12601 ttatatttat ctcatttaac ttatccttcc attcattcgg aggttctaca tcacaaataa
    12661 caaaatctaa ttcctctaaa tctgctatct tatacatact agcaattcct attttagaac
    12721 tatcacataa tacaaaactt tcctttgaat tttttatata tgcttttgac aacaatgcct
    12781 tattttggtc tatactacta attccaaaac tatcaagtat accttcacta gatataaaag
    12841 ctttatctac gtataagttt ttcatagcat ctattgatat tggacatgct gtcctttggt
    12901 gttttaaatt tacttttcca ccaataaata taacttctcc atcaaacaaa ccttgattct
    12961 cgtaagaaat caattttgac agaactggat aagagtttgt aataattttt aaattgttta
    13021 cagatattat ataatctaca atctgcaaat tggtagtacc ttcatctatg ataattgact
    13081 cattgtcatt tacaaactta gctgcatatt tagcaatact ttgcttttta tctaggttag
    13141 tattgtttct ctttatatgt tctggttcaa tcactccata atttattttt atagcaccac
    13201 catatacctt ttttaattta ttttcatttt ctaattcttc caaatatctt cttattgttt
    13261 ctgatgatac ttctaatctt tttactaatt ctttagtgtt taccttccct tttgaatcta
    13321 gtaatttaag tatttctttt tttctatcta tcgaatttaa agacattcat tttcctccta
    13381 aatataatat atttttctcc tataatatta caatgatacc taagatttat gaaatcatat
    13441 ttatgtatta aattttttac ttaattaaag ttattttcaa aagactttgt attatcatta
    13501 aaaaagtcca tcaaatatta gtatcagatg gactttgtaa aattttattt tataaattaa
    13561 tatttaaagt ctaactacta atttattttc ttgtctccaa gaattttact aaattattta
    13621 cagtagacat atcttttctc tctaaatcag ttggttgaag ctctatccct aatctatttt
    13681 caatttcaag taaaacttca attatagcta atgagtctaa aagttccgtc tcaaataaat
    13741 taaggtctaa atcttctctt atctcatcag tccctaatac atcttcaaat atttctataa
    13801 ctgtttcttg cataaatttt cctccttaaa attaagctaa ataacctgag aatatcagca
    13861 atccaaaaca cacaatatgg aatgtaatga aaatctgcac tctctcgaac catttatctt
    13921 ttttatgttt tttataaaat ttagattttc gctgatatat atcagttaac acaagtgcca
    13981 atccttggta tacaccataa gcaacataat accaagttag accatgccaa aatcccattg
    14041 ttatcatggt tatcatctga gccacatgag cagcagtagt ccttttctta aatcttttct
    14101 ttctcattga tgacataaca aatctagaaa aaatataatc tccaaaccat cttgaaagac
    14161 ttatatgcca tcttgtccag aactctttca tatctttgct gataaatggc ttgtcaaagt
    14221 ttataggtac ttgtattcca aatatgtacc ctgtacctat agcaaataaa ctatatcctg
    14281 caaaatcaaa gaacaggtat aaactatatg cgtacatata acttaaaata tttataaaac
    14341 tcatatcttt aggtattctt gatacccaat atgtatttat caaaaatgct ataacaaact
    14401 tataacctac tcccatgaca atatttttta ttcctggcag taagtattct tctatatact
    14461 cttttctaga gatttgtttt tccaagtcct gttcaaatct tcttgaccta tcaataggtc
    14521 cagaacttaa agttggaaaa aataacataa aataaagcat ctttgaaatt ttaacttctt
    14581 ttatagctcc atcataaatt tcaataacca tttgtatagt tctgaaattt aagtatgata
    14641 ttccaataaa tccaattatt ccaaaagaag taactggaga tattttattt attattattg
    14701 ggagcattga tgcaaacaag aatccccaat atatgtattt atttttagtt tttcttctta
    14761 catattcata tcctttaact attattactt caagaataat aaatattacc aagtacttta
    14821 gttggacatc aattcctact attaagaata tcataaacaa acttgccaac ataccataat
    14881 actttatatt cttgcccatt agacctaaaa ttactgctgg tattgatgtt aataataata
    14941 tataaagata aaaataatct ccatattgtg aaaaaatcat taaatttcct ccataagttt
    15001 ctttctgtct attttaccat tgatatttgt tggaaactca gagattattg aaatatttct
    15061 tggaatcata tatgaaggta tatactttcc aagttccttt tttattatca tcccattttt
    15121 aatattactt aaatcattct tttcatttaa ctcaacaata ccttttaagt aagcaatttt
    15181 ttcgtcctta tatactggta aaactactgc attttttaca ttatgtactt ttctcaaatt
    15241 attttctata tcttctattt caattctaaa tccatttaat ttaatctgaa aatcttttct
    15301 tccacaataa tatatatttc catctaaaag atatcccata tctccagttt tataagctct
    15361 ccactttaca ccatctattt catcatagaa aaatacttca tcagtctttt ctttattatt
    15421 gaagtaacct ttagaaactg atggtccaat tattataatt tcaccctttt cattttcttt
    15481 aagctcattt ccatcttcat caagtatttt tattttacaa tttgacatag ggtatccaac
    15541 tggaagactt ttttcatcat ctatagcctt ttgagtcata tcatttacac ttacaccaac
    15601 agtagcctcg gttggtccat atccattgat aattcttgtg tttggaaatc tactcataag
    15661 ttctttagta agattttttg aaagtgcttc tcctatgaat atcattgact caaggttagg
    15721 aagcatttta ctattaaact ctttttcagt tatacatact cctgcaaaag aaggagttga
    15781 cacccataca gctatatctg agatactaaa ttgtctaaat agttctttat agtctgctaa
    15841 tacatctttt gatattgaaa ataatgtagc tccatggata agaccagggt atattgttgt
    15901 aactgaaagg tcaaaagaat atgctggttg gttcattatt actttttcac ttccatctat
    15961 gtttagatat gggcttatcc aatctgaaaa gctatctaaa ttgtttgaac ttatttgaac
    16021 cccttttggc tttcctgtac ttcctgaagt aaatagtata tatgcatttt catcatcttt
    16081 tacccagttt tctttatcta atgattttcc ctgatattca tttattatat aatttaattt
    16141 atccatatca attacattga tatctccaaa atttctttca tcactaaagt taaataatac
    16201 ctttggtttt atctctttag tgacttcaaa aactctatca attggaaaac taatatcaag
    16261 aggcacatac gctctacctg attttaaagc tcctatcata catgccatta tcatgttttc
    16321 tttatttcca taaattacta taggagtatc ttcttctttg tatacatcct ttagaaatac
    16381 tgatatagca tctgaatatt catttaaatc tttataagaa agtttatctc cattacacat
    16441 tagtgctgtt ctatctgtat ttgaatattt ttttattcct tcaataattt tcatataaga
    16501 atgctccttt aaaatccgtt ataaataaat ggcaattctt taaatggagt aaatgttaga
    16561 actcctatcg ccaatattat aaccatagtt aaacaaatac tagttaacaa atctttattg
    16621 ttctttaaat attttaaata atctctcaca gacatctacc catcctttcg taccaagatg
    16681 cattacatcc cttaaataat acttatctga ccctttatct ttaaggttta gaacttcaaa
    16741 gcctttgctt tctgctattt tttgtgcttt atcgtagtac tgatttcttt ctttttcaga
    16801 aataccagtc aaattataga atttatccat acttgggatt aatacaacaa ctggcttaat
    16861 acctaagtca gtacatacat ttagcatcag tttataattt tcaaactcct tagatgttaa
    16921 taaatttaca tctttgtctc ttcctttgta ttgctctata ccatctttaa aatgctcttt
    16981 ataatagtac ttatcaatat ttagtggatt tttaccaact ctttttttag catcttcaat
    17041 ggctttcttt ctctcatatg accaatttat tggtcttttt ctttttgttg ctctcttttt
    17101 atctagctta attaatcgtt tataaagaat acccttttct ttaagagcta tacaatactt
    17161 tcttccttga aaataaggct ctaaaagcac cttttcagct ttttcaggaa atgtcttagg
    17221 ctcatatagc ttagcatata atgcttctgc tttatactcg tcacttcccc ataataactt
    17281 acttgatttt tttgcatatt caatcttatt ttgtttagat atttttggat tatctaaaaa
    17341 tcgataaaat tgtattggtg aaaatctaga ctgataatgg tgacttgtga ctccgtcttt
    17401 ttccataaac cattgcatag aaattaacaa gacaactttc ttattatcaa tattaggatt
    17461 catacttcct aatattgcag catcttgtaa tgtctgagta taggctctcc caattgcaaa
    17521 caccttattt ttactcctat ttgtattaaa atagtacttt ggatgttgtt ttgttgaatt
    17581 actaagttct gatgacccca aaatcataat atccttttct cttaaaaaat gattatttgc
    17641 tgtaacccct ttatctttta tatcacttaa agtatcatcc ataattggaa gtagattttt
    17701 ctccctcaaa agttcatcag ttttagaatc aagaaatttg tctaacccaa ataaaaatac
    17761 tacgcctatt ataaaaggtg taataaagta tatcaatttt ctcatatttt ctctcctcta
    17821 aaaatattca aatttatgtt attattaatc atattagttt tgatatgtat attcaactac
    17881 aaaattgtaa taaaatcgtc aaaacttttt ttgatactgt tacaaaattg taaaaaaaaa
    17941 gaaaaaaacg ccaaaaataa atttcttttt agcacttttt catacttata atttgacatt
    18001 ttaacacttg gttaagtata caccttacct taacttaaaa ttaaatttat ttaagaatat
    18061 atattataaa ttcttatatt ttctaatata aactatatac tatcagctgg ataaacaata
    18121 ctactttgtc ttcttatctc atccataatt tccattacta ttattgaatt ttcatgagaa
    18181 tttatatttg attctatttt attgttattt ataagactta taaactctac aagttcataa
    18241 tacattcctt ctatttctct aggcttttcc tctgcttttt cttttggaat agatatatct
    18301 tcttctcttc catctctgta tttaataata gctttttcaa atagattaag cttttcaatt
    18361 attatacttc cctcttctcc ctgtatttca gatggtatat aagaatcagc tactttagaa
    18421 tactgaatta tcgcatccat atcatcatat tgaaatacag ctgaaccttc tccatccact
    18481 ccagtttgaa gtatatgaga aactgccttt acagtttttg gagcaccaaa taaattcacc
    18541 ataggatgta tacaatacac tcctatatcc attaaagctc catttgacaa ctctttttta
    18601 aaggcatttt ctataattcc atttttatat ttatcatatc ttgaagaata ctgacaatag
    18661 cttccaaaaa atcttcttat tttccctatt ttatacaagt tttctttaac tgctttaaag
    18721 tttggaacac atgtaattct catagcttcc ataagcaata cattattatc tttggctgtt
    18781 tttatcattg atttgacttc ttttaaatta gatgctaacg acttttcaca aagcacatgt
    18841 tttttgtgtt ttaaacaagt aatggtttgc tttgaatgta atgcatttgg agaagctatg
    18901 tatacagcat ctatatcctt cgatttagcc atttgttcta aatcatcaaa aaaaatactt
    18961 gctccatata gagttccaaa ttctctagct ttttctaaat ttctagaata tacagcaacc
    19021 aattcaaagt ctttaaccct agatgcagct cttagaaata tatttgctat attgcttgtc
    19081 ccaacaatac caaatcttat tttacccatc ataaatcact ccccattttc tacactttat
    19141 tttaatttta ataaaaaact ttacaactta tagatatatt ataaaataat tttatcaaat
    19201 aaaaaagaaa gctaatttta aatgatttca ccattttaac atagctttct tttaactaca
    19261 atctaaattc aacttatatt tttaaagtta tctttctact atatctttat ttcccctcaa
    19321 aaattttata gtaagaattt catccacaaa caatgttact aaaagtatag ttacaacaat
    19381 tggccaataa ggtattataa gactatataa actatcaact atatacactt gaaaatatct
    19441 aactccaata aatgtcaata tagtccaata tacagaaaac tttagacata ctattcctct
    19501 actattgtac ttataatctg aataatccca atagacctta ttaaaatata ctttcataag
    19561 tattcctgta atatattcta ctactgtcgg cactactaga cataataaaa gcaatgcaaa
    19621 tatattattg gggtcaataa atcttgagac ctctattata atcgacattg caattgcata
    19681 cattggttta aatggaccat ttaaaaatcc atcttcttga aaatgccctt gtttataata
    19741 actatataag ttttcaatta tccaacctat aaaaccatac aagaaaaaat taaataaaaa
    19801 atataataga gaacctacat tatccatgaa tattcctcct agctgtttat acttattttt
    19861 tctcatattc atggatttat tctaaatcaa agaaattata tttaatttat ttaattaata
    19921 ctttatatta tagattgtga cattctttag agttcaaact ttttaggtat tctctaactt
    19981 ccagtaaatt ttcaagtatc ttatgtgatt gtgatttaat ttgctcatct ggctctttta
    20041 aatctggtac atttatacat cttataccac cattgtatgc agcttcaact cccataggcg
    20101 aatcctctat gacaatacaa tttttgggat taacatttat ctttttagct gcttttagga
    20161 atatctcagg gtttggtttc gagtttacaa catcatctcc acaaactata gcatcaaagt
    20221 aatcttttaa atttgcctta gctaatctct ttactgctct ttctctcttt gttgatgttg
    20281 caacagccat tttgtatcca ttttccttta aaaaagagat tagttcattg actcctaatt
    20341 tgataggagc tcccttcctc tccataaatt caatcatatt tttagttttt tcgtcatata
    20401 aatctattat tggtacagaa ctatcgtata tatctgtaag accttctatt attcctttac
    20461 gatttcttcc cataactgat gtataaattt ccttagtcat agtatatcca tacttttcaa
    20521 acgtctccat ccaaaattca agagaaattc tctctgagtc aaacaaaact ccatccatgt
    20581 caaaaataat tccttctact ttttgcatac aattcctcca atattttttg atataattat
    20641 attacacttt ttatgattaa ttttacaacc ctcaatttat ccaattctaa tattgtaaaa
    20701 ttgtaatttt caaaagttac actttggttt actttaatat ttgaaccaag catataggat
    20761 atccatccac ctatactgtc aaatccatca tgctctatat caagtccaaa atacttatta
    20821 atatagttga tagattcagt tccatcaact aagtaagaac ctgctcctat tttcttaata
    20881 tgagtctctt ctttatcaaa ttcatcttga atttctccaa ctatctcttc tagtatatct
    20941 tctattgtta ccacaccaga tgtaccacca tattcatcca caactatagc aagttgaagt
    21001 ttttcctttc ttattctctc aagagcttta tctatagtta aattttcaga tatatatata
    21061 atatctctca atatttcttc tagttcaatt ttattttcat ttatcttttg attgtataaa
    21121 tctcttatat gtacaaaacc taatatatca tctttatttt ttctgcatac tggatatctt
    21181 gtaacacctt cctcttttaa aatagctaat atcttttctt cagaatcact ttcatatata
    21241 cataccatat cagttctagg aaccattatc tctctaattt tcttttcttc aaattcaaaa
    21301 atattatcaa ctaatctttg ctctgattca tctataagtc cacttttata actttcttct
    21361 actaatagtc ttatttcgtc acctgtatgg ggttcatcaa cctcatcagc ttgtgaatat
    21421 ccaaatggtt taagaaataa atcagtactt aagttgaaaa aatatattat tggatatgtt
    21481 aacttataaa atcctactaa taaaaaagat gtactcaaca ttattttttc tgtattataa
    21541 agtgctaaag ctttaggaac caactctcct attacaacct ccatcattgt tataattaaa
    21601 aaagaaatgg aaattgatat agtatatata atactttcac taagattaaa tacacttact
    21661 attggtaaaa ttaactcttt aactgtagat tctcccatcc aacctaatgc aagtgaacaa
    21721 agtgttatac caagttgaca tgcagaaaga taggaattta agttgtcttt tattatttta
    21781 catctttttg cattattatt accttcagtt atcatggtat caatcctaga actacgaagt
    21841 tttaccatag caaattcaca agctacaaaa aaagcattta tcaaaacaag cattactata
    21901 agtgttactt ttaataactc ccgcataaag tataggttct tataatgtaa gaaatctata
    21961 agaactcaaa gttcccccct tatattaata aatttaattt ataatatata ttctatcata
    22021 tttattaata tatcctactt tattacaaat aatttttata aactaaataa ccccttttaa
    22081 tatttatgac tggctatttg ataaatatta aaggggttat ttag
//
LOCUS       DAMJWU010000057        21175 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMJWU010000057 DAMJWU010000000
VERSION     DAMJWU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.057"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            242..763
                     /locus_tag="PC460_002322"
     CDS             242..763
                     /locus_tag="PC460_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601203.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            797..1573
                     /locus_tag="PC460_002323"
     CDS             797..1573
                     /locus_tag="PC460_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2601204.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1915..2868
                     /locus_tag="PC460_002324"
     CDS             1915..2868
                     /locus_tag="PC460_002324"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF2601205.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2886..3905
                     /locus_tag="PC460_002325"
     CDS             2886..3905
                     /locus_tag="PC460_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF2601206.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3936..4187
                     /locus_tag="PC460_002326"
     CDS             3936..4187
                     /locus_tag="PC460_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601207.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4469..6274
                     /gene="lepA"
                     /locus_tag="PC460_002327"
     CDS             4469..6274
                     /gene="lepA"
                     /locus_tag="PC460_002327"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF2601208.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6423..7760
                     /locus_tag="PC460_002328"
     CDS             6423..7760
                     /locus_tag="PC460_002328"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF2601209.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7993..9306
                     /locus_tag="PC460_002329"
     CDS             7993..9306
                     /locus_tag="PC460_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2601210.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9478..10653
                     /locus_tag="PC460_002330"
     CDS             9478..10653
                     /locus_tag="PC460_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF2601211.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10756..11775
                     /gene="hrcA"
                     /locus_tag="PC460_002331"
     CDS             10756..11775
                     /gene="hrcA"
                     /locus_tag="PC460_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF2601212.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11805..12425
                     /gene="grpE"
                     /locus_tag="PC460_002332"
     CDS             11805..12425
                     /gene="grpE"
                     /locus_tag="PC460_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF2601213.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12517..14364
                     /gene="dnaK"
                     /locus_tag="PC460_002333"
     CDS             12517..14364
                     /gene="dnaK"
                     /locus_tag="PC460_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF2601214.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSESEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14575..15729
                     /gene="dnaJ"
                     /locus_tag="PC460_002334"
     CDS             14575..15729
                     /gene="dnaJ"
                     /locus_tag="PC460_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF2601215.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16695..17630
                     /locus_tag="PC460_002335"
     CDS             16695..17630
                     /locus_tag="PC460_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2601216.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKKRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17666..19039
                     /locus_tag="PC460_002336"
     CDS             17666..19039
                     /locus_tag="PC460_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2601217.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19227..19994)
                     /locus_tag="PC460_002337"
     CDS             complement(19227..19994)
                     /locus_tag="PC460_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601218.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(20024..21133)
                     /gene="ugpC"
                     /locus_tag="PC460_002338"
     CDS             complement(20024..21133)
                     /gene="ugpC"
                     /locus_tag="PC460_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF2601219.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         8269 a         2391 c         3740 g         6775 t
ORIGIN      
        1 tatgcttaat taagataatt tttccaataa tcaagataat ttttttcatt agtatgtatg
       61 atagatgcat caaacaatat aactaatatg ttagaaggtt atatgtaatt aatgtcagtg
      121 tttatataga atagcatttg tagcatttat tatagttgta attactattt atatcataga
      181 cttgaatagt ctaaaaaaat agactataat atttttatag actaaagaaa gggctgataa
      241 gatggacata gcaaaactaa ttttaaatcc tgcaaggctt cgtattttac aatatattcg
      301 actttatggc agtgttcgta catctgatat tgtaaagtat ttaaatgata ttcctcgtgc
      361 aacagtttat caccatgtaa aaattttaga agagaacaat atgattgaag taattaaaga
      421 aaatcgtgtg cgtggtacta ttgaaaaggt ttatgcacta aaggaatata caacatctat
      481 ggaaggagag accttcgttg cactttctac agcattttat gtaggtctga tgcaggaaat
      541 gaatgaatac tttagcagaa aagagcaaga ccataaaaag gataatgtat ttttctcttc
      601 agcattacta tatatgagtg ataatgaata tgaaaatctt cttaaaagta tagctgattt
      661 attgaaacca tatatagaac aaaaacctaa atcaaatcta aaacttagaa aactttcaat
      721 tatatcttct ccacctgtag ctgataatga taaaacaaaa taacatgcaa cccattttta
      781 taaaaggagg ttatatatga aatttcatga gtttggaaat aaaaacagac cacatattat
      841 gttgattcat gggggaggga atgcatggtg gaactatttg cgacaagcag aagttttgtc
      901 taacaaatat catgtgatac ttcctacatt ggatggtcat ggagaagaat atgaaacaac
      961 atacatttct acagaggata cagcagataa gttgatatca tatatagatg aaaattgcag
     1021 tggacatctt ttcgctttgt gtggggtatc acttggtggt caaattgtca tggaactttt
     1081 atctcgaaaa cctaatatta ctaaaaaagc aattattgat ggtagtattt gctacccaag
     1141 acctcttatg gcaaggtttt gtattgctgg tattcgattt tttggaggac ttttgtttag
     1201 tgagaaggct tgtcgctttc aaattgctat gatgccaaaa ttgttacctg aaaatatgca
     1261 atatccagat gaaataaaag cgtattatat gacagatatg ccacatgtaa gaaaagaaac
     1321 attctataat atgtatcgta cttacatgat gaactataca cttaaagaga atgttaaaaa
     1381 aaccacagca caagtaatgt actggtatgg agaaaaagaa atgaagtgtg tcaaaaaatc
     1441 tgcaaaactg tttcagtctt atgtgccctc ttgtgagatt tatgaagcta agagctataa
     1501 tcatgggtat ttatcacttt atcttccaaa tgaatggttg gaaattgcag aaccattttt
     1561 ccaaaaggaa taaaggtgtt aggtgattat tgtataatta ttgctgtttt tttatatagt
     1621 aaataaatta ggtataaata taaaaaatta ttttagttaa tatattagtg aaagcttaat
     1681 aggaaaatta taaatagatt aaaggtagca cctatataaa tagttgctac ctttttctgt
     1741 ttgtaaataa atgatagata agaaggttta ctgacaaatc actgactaaa cccaattttc
     1801 ttacattgaa agccttaatt ttacttgttt taaataaatt tacaatttct tgttataaaa
     1861 tactaaattt tgttaacaat tataattagc atttaattta tactggaggt ttatatgatt
     1921 agtgtaagaa cagatttagc tttagaagct agtgaaatgt gtgaaaagtc tcaagaagga
     1981 agtagtattc ctggtgtaaa aatagaaact aaagaattag aaaattgcat tgtaactaaa
     2041 gttgaagtaa tagacaggca aggcgcagag ataatgaata aagatatagg aaagtatata
     2101 actctggaaa gtaatcttat gaagtttgat gatgatgagt caagagaaga aatgataagt
     2161 tatttaaaag aggagttagt agatatattt ggccaagaca aaaataaaaa aaccctggta
     2221 attggtcttg gaaatagaaa tattacttct gatgcgttag gaccaaagag tgtatcaaaa
     2281 acacttgtaa ctagacatct ttttaaaaat tataataaag actatgatga tgatttcact
     2341 gaggtatcag ctttaagccc aggagttatg ggtgttactg gaatagaaac tagtgaaata
     2401 gtaaaatcat tggttgagaa ggttaagcca gatagagtag tagcaataga tgcattggct
     2461 tcaagaaaaa tggagagagt aaactctact atacagatat caacagcagg aatatcacct
     2521 ggtggaggtg ttggcaatac tagaaaatct ctaactaaag aaactttagg cgtggatgtt
     2581 attgcaatag gcgttccaac ggtggttgat gcagcaactc ttacaataga tgtcttagat
     2641 atggcaatag acaatttaat agctcaatca gaagaaacag aaagttttta tgaaatgttg
     2701 aaaaaactta aagaagaaga aaaatatcat ctaataaaag attcgttaga cccatatgac
     2761 aagaacttaa tagtaacacc aaaggatata gatgatacaa tcgaaaatct atctataata
     2821 ataagtgaag gcttaaatag gtctttacat ccaggtagat tggtataaaa taaggaggag
     2881 caattatgtt taaaaaatgc attaaagtag taaccttgac ttttatatta gtatgcattt
     2941 taccaggaaa atcattagct ttgaatcaag atgatttttt aaagttttta gtaaattcat
     3001 cttatccaga agctaaagtc gaaggaaatg atacagaaaa taaaaagaat aataaaaata
     3061 aagaaacaag taaggaaaat aaggaagaaa gtaaagaagg aaacactaaa tctaaagatg
     3121 caagtaaagt tgataataaa aaagagagtg aaaaagaata tataaaactt tatgttggta
     3181 aagaaaatgt tccagatatt gagtcaaaaa attcagacac aactgaaact aacacaacat
     3241 ctagttcaga ttataaggat gatttaagag taaccaaaga aaatccaaga attttgatat
     3301 atcatactca tggatgtgag acttattcaa attcacctga tggaaattat cattcaagag
     3361 ataagaaaaa ttcagtaatg gaagtaggaa gtgcattaac tagtgcttta gatagcaaag
     3421 gatggggtgt agtccatact actaaatacc atgactatcc atcttataat aactcttatg
     3481 caagtagctt aaaaacaata caaagtatat tacctaaata taactcagtg gatatagcga
     3541 tagacttaca tagagatgct agggatttaa ctaatccagc cactaaggaa aaagaccatt
     3601 taaaatatac aactatgata aatggagaga gagtatcaaa gttcttcttt gttgtaggag
     3661 gtaaaaatac aaatagaaaa caacttagag ctttagcaga agatataact gcttttgctg
     3721 agaaaaagta tcctggtcta gtatctccaa tagtagaaaa agactatgct agattcaatc
     3781 agtttgcagt taagaatcat atgttagtag aaatagggaa taacgcaaca agtgtagaag
     3841 aatctaaggc tactacaaag tatttagccg agattttgga tgaatatttt aaacaaaaaa
     3901 attagagctt taagttataa ttaggagcat gctaaatgag ccgattagaa aaaaatatag
     3961 aaagtaaaag taaactaaga aaatacaagt atattatgag aatcttattt ttgattacta
     4021 tgattatatg tacatcagtt tgtgtattta tagtggacag taatgcaaaa agtatgctag
     4081 gaatgaaaca atatgatttt ccaagtatta aatttgatat aacaaaattt gattttgaca
     4141 taccaaattt tgatatacat gaaatcataa aaaaatttaa taagtagtaa taagtgaata
     4201 aggtttattg attaaaataa gccttatttt agttatatat ttttaatgta taaaataaaa
     4261 attcattaac tgtattttat aggatttact ttaagtttat gatattatat aatatttagt
     4321 gttagacttg ataatccaca tataatgtgg ataattttgt ttagtgccaa ttagtttata
     4381 ttcaaaaatt ttaaatttgg tatataaaga gtttttagat aatgtaaaat aataactata
     4441 agaataatca agaggaggga attttaaggt ggacaataaa caaagtagaa ctagaaattt
     4501 tagtataatt gcacatatag accatggaaa gtctacctta gctgatagat taatacaaca
     4561 aactgggctt gttagtgaaa gagacatgaa aagtcaatta cttgacaata tggaccttga
     4621 aagagaaaga ggaatcacta taaagcttca aaacataaga ttaatgtata aagcgaagga
     4681 tggaaatgaa tattatttaa atcttataga tacacctgga catgtagact ttaactatga
     4741 ggtatcaaga agtttagctg catgtgaagg agcactatta gtagttgatg cagctcaagg
     4801 tgttgaggca caaactttag ccaatgttta tcttgctata gaccaagatt tagaaatatt
     4861 accaataata aataaaatag atttaccaag tgcaagacca gaggaagtaa aaaatgaaat
     4921 agaagattta atagggcttg attcaagtga agctcctctt atttcagcta aaactggttt
     4981 aaacatagaa gatgttttag aagatattgt aaaaaatgtt cctccaccta aaggagataa
     5041 tgaagctcca ttaaaggcat taatattcga ttcttactat gatgcttaca aaggtgttgt
     5101 agcttatgtt agagtatttg aaggaactgt taaaaagggt atgacaataa aaatgatgaa
     5161 taccaataaa aagtttgagg taactgaagt tggtgttatg gctccaggtc aaactgaact
     5221 tagtgaatta tcagcaggtg atgttggtta tattgctgct agtataaaag atataagaag
     5281 ttgtcgtgtt ggtgatacaa taacagattc aaataatcct acagaagaac ctttaccagg
     5341 atacaaaaaa gctactccaa tggtatattg tggtatatat ccaggagaag gagaaaagta
     5401 tgagaatgtt agagatgctc ttgaaaagct tcaagtaaat gatgctgcac ttgaatatga
     5461 agctgagact tcagcagcac taggatttgg ttttagatgt ggtttcttag gtcttttaca
     5521 tatggagata atgcaagaaa gacttgagag agaatttaac cttgatataa ttactacagc
     5581 tccatctgtt atatatagag ttacaaaaat ggatggagag gttgtaatga tacaaaatcc
     5641 tgcaaactta ccagaaccat ctgagataaa gatgatagaa gagcctatag taaaaggaga
     5701 tataatagtt cctaaagact atgtgggtgt tgttatggag ctttgccagg aaagacgtgg
     5761 aaatatgtta aatatggaat atatagatga tagaagagta atgcttcact atgaccttcc
     5821 tctcaatgaa gttgtttacg atttctttga tgctttaaaa tcaagaacta gaggttatgg
     5881 ttctcttgat tatgaggtta aaggttatgt agcatctaca cttgtaaaac ttgatatatt
     5941 aatcaataaa gagcaagtgg atgcacttag ttttattgtc catgagacac gtgctttccc
     6001 tagaggaaag gcaatgtgtg agaaattaaa aggagaaatt ccaagacatc agtttgctat
     6061 accaatacaa gctgctgttg gtaataaggt tattgcaaga gaaacaataa gcgctctaag
     6121 aaaagatgtt cttgctaaat gttatggtgg agatatatct cgtaagaaga aacttcttga
     6181 aaaacaaaaa gaaggtaaga agcgtatgag acaaattggt tctgttgaag ttcctcaaaa
     6241 agcatttatg tctgtgctta aattagatga gtaaatcctt ataaatttag ataaagatat
     6301 ttaaaagcta gagtgatttt agaattattt ctaaattagt ctagcttttt tatggtatat
     6361 tttaagtaaa ctcatagaga atataagtaa gaatattaaa gaagaataaa aaggtgatat
     6421 ttatgattaa tatagaaatt cctaaaaaag tagactatat aattaaagaa ttagaaaaga
     6481 atggttatga agcttatata gttggaggat gtgttagaga ttgtttatta gaaagagttc
     6541 caaacgattg ggatataaca actagtgcta gaccagaagt ggtagtagaa ttatttgaaa
     6601 aaacaattcc aactggtata caacatggga ctgtaacagt tatgattgaa catgaaccat
     6661 ttgaagtgac gacttataga attgatggaa attatagtga tggtagacat ccagattcaa
     6721 tagaatttac aaataacata gttaaggact tgtcaagaag agattttact atcaattcaa
     6781 ttgcatacaa ttcaaagaca ggattagtcg acccatttaa tggctatgaa gatatacaaa
     6841 ataagtatat acgttgtgtt ggaaatccag tagatagatt tgaagaagat gcacttagaa
     6901 tgcttagagc agttagattt tcagcacaac tcaattttaa aatagctgaa ggtactaagc
     6961 aaagtattca taaaaaagca gatttgataa aaaatgtttc tattgaaaga atccagacag
     7021 agtttaataa aatattagta tctgattcat ctaaattaaa cttattaaaa tcaacaggtt
     7081 tgttgaaatt tataattcct gaaatatgtg aattagaaga tgttactcaa cacaatccat
     7141 atcacatcta tgatgtacaa aaacatactt taatagctac agaagtaatt gaagatgaac
     7201 tgtatttaaa acttacaatg ttgtttcatg atttaggtaa aaaagtaact aaaacaactg
     7261 ataaaaatgg tgttgaccat ttttatactc atagtagaga atctgtaaag attgcaaaga
     7321 agattttgaa aagacttaag tatgataatt atactattaa taaagtttta atattaatac
     7381 aatatcatga ttatagaata gaacctaaaa ggaagataat taaaaagtta ttaaataaac
     7441 ttgaagatgt tgaattattt gaagatttaa taaaagttaa ttgggctgat acacttgcaa
     7501 aaaatccaaa gtatgcaaaa caaaagatat taaatcttat tgagtgtgaa aaagaattta
     7561 aacatataat aaatcaaaag gagtgcttta atcttaaaga tttagctatt aatggaaaag
     7621 atttgatatc tataggtata aaaccaggta aagatatagg atatatttta aataagatgc
     7681 ttgaaatagt cataaataat ccagagttaa atgaaaaaga aatactaaaa gaaaaagcat
     7741 taaatatata tacattttag attcaaatat aaaaaattta attactttaa aaaacataga
     7801 ataaaatact tatttagata aaataaataa tttttagaga atattttaga agttacgatt
     7861 gtatgatttt taaatgcata ggcatttact ctaggactat agactaatat aaaaaaaaac
     7921 tttgggaata ctaaaaccat aaatttataa aattaatatt ttatatactg taaaacagtg
     7981 aggagggaca gtatgcatac tcaactacaa aagactattg gtctttcagc agctctttct
     8041 actgttgtag gtatggttat aggttcggga gtttttttta agccacaagc aatatacact
     8101 acaactaatg gagctccagg tctaggtatt atagcgtggc ttttaggtgg ttttataaca
     8161 ataactgctg ggcttacagc tacagaaatt tcagctgcta taccaaaaac tggtggtatg
     8221 atgatttata tcgaagaaat atatggagaa aagttaggtt ttttaactgg atggatgcaa
     8281 actgtattat ttttcccagg tacatcagcg gcattaggag taatatttgc tcaacaagca
     8341 tctgaacttt taggaatgag cccaaataat atggcaaatg tacttcctat agcaattgga
     8401 gttatactat ttttagcgtt attaaatata ataggttcat ccttaggagg aaaagttcag
     8461 acagtagcca ctattggaaa gatgatacct ttaattttaa taatagtctt tggatttatt
     8521 aaaggtcaaa gtagtgaagt attaaatcca tttgtaggag atggtgtaaa tgcatcaaat
     8581 gcacttggac aagtattaat agctactttg tttgcatatg atggttggat aaatgttgga
     8641 gctatatcag gagaaatgaa gtctccagag aaagatttac caagagcaat agttggtggt
     8701 ttatcattag ttatggcagt ttatattata ataaatatag cttatctttg ggtagtacca
     8761 gcaagtgaat tagcaacagt tacttcccca gctacattag tagcaacaag attgtttgga
     8821 aatataggtg gaaaagtaat aactgtgggt atattaatat cagtatttgg gacattgaat
     8881 ggatatttat taacaggttc aaggatacct tatacattag ctgaaatggg aactttacca
     8941 gcatctaaaa ctcttttaaa agtaaattct ggaggttctc ctgtaaattc aatactactt
     9001 ataactgtgt tagcctgtgt atatgcatta tctggtcaat ttaatctttt gacagactta
     9061 acaatatttt caatatgggt attttatgta ttgactttta taggtgttat gaggcttaga
     9121 agacaaaagc cagatttaca cagaccatat aaagtccctt tatatccaat aataccaatt
     9181 atagctatat taggtggact gtttgtaata ataaatcaaa tattgacttc aacagtcata
     9241 tctttaggcg gaatatttat aacactatta ggtttaccag tttattatta tatgaaaaaa
     9301 agataactac ttaatttaag ttagataaat ttgggtaaag gtgaaaatta acttttatcc
     9361 aaatttttta tactgttata caatataagt attatatatt gcttgatgtg ctattttgtt
     9421 tgatttataa tttagtaatt gatgttttaa gataatttaa gaaatgagga gtgaaagatg
     9481 ctaggacttt atgtacatat tccattttgt gtgaaaaaat gtaagtattg tgattttaat
     9541 tcttataaaa tggacataga ttcaaaaaaa agatatatag aagacttaaa aatagaaatg
     9601 gaattatata gtaataaatt atataaagat aataaatata aaaataaaga atgctgtagt
     9661 ctaaataaaa atgacaaaat aacgagtata tttgtagggg gaggtacacc tagtatacta
     9721 acctctgatg aaataagaga agtatttata agtataaaag aaatgtttga tatagatgag
     9781 aatgcagaaa taactataga gtgtaatcct gggacattaa cattagaaaa attaaaaact
     9841 atgaaagaga ttggcataaa tagacttagt atagggctac aagctattca agaaaagcat
     9901 ttgaacttta taggaagaat acatacttat gaagaatttg agaaaaacta taaagatgca
     9961 ttaagtgtag gttttaaaaa tattaatata gatttaatgt acagtcttcc aaatcaaact
    10021 ctatgtgatt ggaaggaaac tcttgaaaaa gttgtacact taaatccaac acatatatca
    10081 gcttattccc ttatactaga agaaggaact gaactataca atatgtatga aagtaataaa
    10141 tttgaactta tagatgaaaa tgtagatata gaaatgtatg agtatacaat aaactactta
    10201 aagtcaaaag gatataatca atatgaaata tccaactact caaaagaagg ctataattgt
    10261 gagcataata ttttatattg ggaatgtgaa cattatatag ggattggagc aggagcttct
    10321 ggctatatta atgagaacag atacaataat gtagaatctt tagaagatta tcatttaagt
    10381 ttggtaaaaa gagaaaaacc aatacaagaa aatgaaattc tttctgagaa agatatgata
    10441 gaagaaaaga tatttatggg gcttagaatg aataaaggca taaaatttga agattttaaa
    10501 aaaaaatttg gaatagattt tagagagaag tataataagc aaatagaaat gctattagct
    10561 agaaagctta taaatcagag ctttgaaggt atacaattaa ctcaaaaagg aagagaaata
    10621 tctaatagtg tatttataga atttatggaa taatttttaa ggtgttgaca aaataaattt
    10681 ataatggtat ataatatata taatcttttt agcactcgaa cttagtgagt gctaacagct
    10741 aggaggtata ataatatgga attaaatgaa agaaaattaa atatcctcaa agctatagtt
    10801 aaagattata tagaaacagc tgaagctata ggttctagaa caatatctaa aagacatgat
    10861 ttaggtgtta gtgctgctac cataagaaat gaaatggctg accttgaaga attagggtat
    10921 ttaattcagc ctcatacttc tgcgggtaga gttccatctg aaaaaggcta taagttatat
    10981 gtaaactctc ttatgagtaa aagcgaattg gatgataatg ataagattct tattgaacaa
    11041 tgtatgaatc acaatataaa tcatataaaa gaattgattc atgaaacttc taagctatta
    11101 tcgcaactaa caaactatac aactgtagca gttactaaga gtttaataaa tcaaagtgtt
    11161 ataaagcaca tacagttggt tgctatgaat gacaataata ttttacttat agttgtaaca
    11221 gataaagggg acttaaaaaa agctaacctt acaactaatg tatatttaga tcaatctaag
    11281 ttaaatttga tttctgataa tcttacaagg aagctcttag gtaaaagtat aacagatttg
    11341 gatgataatc taattgcttt tattaaatac gaaataagtg agtattctgg gcttatagat
    11401 gagcttttaa atgctttgaa ctcaaatatg aaagaagaag atttttctct atcattaaat
    11461 ggagctacta acatatttag ttatccagaa tttaatgatg tattaaaagc aaagtcattt
    11521 ttaaatatgt tggaaaaaaa ggaaacaata gctgacataa ttaaatcaaa gggaatacaa
    11581 aaagacaatc taaatataat aattggtagt gataatgatt gtgaactggc acaggattgt
    11641 agtatagtta cagcaactta taatgtagat agagacttgg ttgggcgaat aagctttata
    11701 ggccctacca gaatggatta tgctagaatt tactctataa taaattatat gagtttatta
    11761 attaatagaa agtaaagaaa ttttaaattg cgaggtgtaa agtcttggat aaaaaaaatg
    11821 agcaacagga agtaagagaa gaaaatgata cttctattaa ccaagaatcg gaaactcaag
    11881 tagagttaga agaggaagtt gttaatgaag aatgtgaaac atcaagtgaa aaaactgatg
    11941 aaaaagaagt tgatgatgaa aatgtaacag atataaattc taagttagca gaaaaaaaat
    12001 tgcaggatga gttggatgaa ttaaatgata aatatcaaag acttcaagcc gaatatgcaa
    12061 actacagaag aagaactcaa caagagaaag agacaatagg cgtatttgca aatgaaaaaa
    12121 taataactga attgatacca gttatagata gtatggaaag agccttagat gcatgtgaag
    12181 ataaggaaga tactatgtat aagggtataa gccttgtaca taaacaatta atagacactt
    12241 tagtaaaatt tggagtagaa gaaatagaag cagaatctaa ggaatttgac cctaatctac
    12301 atctagcagt tatgcaagaa agtgtagatg gagttgaagc aaatcaaata gtaatggtac
    12361 ttcaaaaagg atataagcta ggaactaaag ttgtaagacc ttcaatggtt aaagtttctt
    12421 gctaagtata aattaaaggt taaattattt aacctaatat aaaatattaa aataagtaaa
    12481 acttaattat ataattactc gaggaggatt ttagtcatgg gaaaaataat aggaatagat
    12541 ttaggaacaa caaattcatg tgttgcagtg ttagaaggtg gagaagcaca aataatagca
    12601 aatagtgaag gtatgagaac tactccatca gtagtagcat ttacaaaaga tggtgaaaga
    12661 atagttggag aacctgcaaa aagacaagca gttacaaatg cagataaaac aataacttct
    12721 ataaaaactc atatgggaac tgattataaa gtaaatatag atggaaaatc atatactcca
    12781 caagaaatat cagcaataat attacaaaaa ttaaaatcag atgcagaaag ttacttagga
    12841 caaacagtaa cagaagcagt tataacagta ccagcttact ttacagatgc tcaaagacaa
    12901 gcaactaaag atgctggtag aatagcaggg cttgatgtta agagaataat aaatgagcca
    12961 acagcagcag ctcttgctta tggtatggat aaattagacc aagaaaagaa aatattagta
    13021 tttgacttag gtggaggaac ttttgacgta tctatacttg aaataggaga tggaactttt
    13081 gaagttttag ctacagctgg taacaataga ttaggtggag atgactttga ccaaatagta
    13141 atagattatt tagcagagga atttaaaaaa gctgaaggtg tagatttaag aaatgataaa
    13201 atggctcttc aaagattaaa agaagcagca gaaaaggcta agaaagaatt atcatcaaca
    13261 atgagttcaa acataaactt acctttcata actgctacag cagaaggacc aaaacactta
    13321 aatatagatt tatcaagagc taaatttgaa gaattaacta gaggtttagt tgaaaaaact
    13381 atggaaccaa ctaagacagc tttacaagat gcaggacttt ctacaggtga tatagatgat
    13441 gtattattag taggtggttc tacaagaata ccagcagtac aagaagctgt taagaagttt
    13501 ataggaaaag aacctcataa aggtataaac ccagatgagt gtgttgcagc aggtgcttct
    13561 atacaagctg gagttttagc aggggatgtt aaagacttat tattactaga tgttactcca
    13621 ttatctcttg gtatagaaac tatgggtaat gtaatgacta agataataga aagaaatact
    13681 acaataccaa ctaagaagtc tcaaatattc tcaactgctg ctgataacca aacagctgtt
    13741 gatatacatg ttcttcaagg tgaaagaagt atggcttatg acaatacaac tttaggaaga
    13801 ttccaattaa cagatatacc accagcacaa agaggaatac ctcaaataga agttactttt
    13861 gatatagatg caaatggtat agttaatgtt tcagctaaag atttaggtac aggaaaagag
    13921 caaaaaataa ctataacatc aaacactaat ttatcagaat cagaaataga gcaaaagata
    13981 aaagaagcag aaatgaatgc agaagctgac aaacaaaaga aagaaaaaat agaagcattc
    14041 aatcaagctg aatctactat atatcaaact gaaaaaactt taaatgaact tggagataaa
    14101 ataagttcag gtgaaaaaga ggatatagaa aaagctatag cagacttaaa agctgtaaaa
    14161 gataatcaag atgctacagc agaagaactt aagaaagcta cagatgaagt aatgactaag
    14221 ttccaaaaag tatctcaaga aatgtaccaa aaagcagctc aagaacaaca agcggcacaa
    14281 ggtgcagaac aagcacaaga caatggacct aaagatgaca atgtagtaga tgctgacttt
    14341 aaagaagtag atgaagataa ataagctata taatatgtaa cactaaatga taattggaac
    14401 atatatgatt tgtgaaaatc atatagatgt taaagatata tggacaatat acatcataaa
    14461 gaatataata tatcatagat tatataactt aaagtagaat ataagaatta atgatagctt
    14521 gtagtttatt attctacaag ctatcgtttt gtaaaatgaa ggtggtgtca atttttgagt
    14581 acaaaaaggg attattatga agtcttaggt atcagtaaag gtgcagaagc tcaagagata
    14641 aaaaaagctt atagaaaatt agcaatgaag taccatccag atagaaatcc tggagataaa
    14701 gaggcggaag aaaaatttaa agagataaat gaagcttatg aagttctatc tgatgataca
    14761 aagagaaaaa cttatgacca gtttggacat gatgggctaa atggtcaagg cggttttggt
    14821 ggtcaaggag gatttggcgg tcaaggcttt ggtggttttg aagatatgtt cggagatata
    14881 tttggtgaca tgtttggagg aagcttcgga ggtggaagag caagaagaag agggcctcaa
    14941 cgtggtgcag atataagaca gagtgttact atatcttttg aagaagctgc atttggtaaa
    15001 aagatgtcta taaaagtaaa tagaagtgaa gaatgtgaag aatgtaatgg aactggagca
    15061 aaaccaggaa catcaaagaa aacttgttca acttgtaatg gaactggtca agttagaact
    15121 gttcaaagaa ctccatttgg taatatagca agttcaagac catgtagtgc ttgtaatgga
    15181 actggagaag tgattgaatc tccatgtagt aaatgtcatg gaactggaaa tacaagaaaa
    15241 gttaagacta tagaagtaga tatacctgct ggtatagatg atggtcaaat gataaaactt
    15301 tctggtcaag gtgaagtagg agaaaaaggt gctccaagag gagacttata tattgtagtt
    15361 aatgttaagt cacatccact atttactaga gatggaaatg atatttattt tgaaatgcca
    15421 attacatttg ttcaagcaac tttaggtgat gagatagaag tacctacttt ggatggaaaa
    15481 gttaaatata gtgtgccaga ggggacacaa actggaactg tatttagact taaagagaaa
    15541 ggaatcccta gaattagagg taattcaaga ggagaccaat atgttaaggt tgtagttgaa
    15601 attcctaaaa aattaaacga taagcaaaaa gaactattga gagaatttgc gaaggagtgt
    15661 gggtcgaacg tccacgaaaa gaagaaaacc ttcggacaaa agatagaaga tatgtttaag
    15721 aaaaagtagt taaagtagcc aatatataat catattttta aaatcttagt tatttataag
    15781 attgtcaaag gagatattat gatttttata ttggctattt ttgtataaaa aataaatgtg
    15841 cataaaataa aactgaatat aaataatttt ctgaaaaata cttgtcctaa aatatataat
    15901 atgctataat ataatcaaat aaaagaaagg ggtgattcca gtgggaacgg atgtggcact
    15961 gccaaatgat gaagaaaact ttattgtaat atacaaaata aaattagata cttttagtgc
    16021 tagattaagt actggaaaaa ctcatgagct gggataatta ttgtaattac ctagactcaa
    16081 acttttagta gtaaaatagt aatattatat agacaaggta tctaattttt aaatcttttg
    16141 tatgaattaa aatttttata aagggtatat ctgattagaa tgtaattagg gaaacctagt
    16201 tgtgaattac ttttagtata tacatgtgta aagaaatatg tatatggtat tgggtattgt
    16261 gctagatgtg tatgtaaagg aatatgtgtc tagttgggta tatgtaaaga aatatactta
    16321 taatctatga aaaattttta gtataaattg ctaaaacatt gagattgttt tttatagaaa
    16381 gttagtttat accaactaaa aatgtgctta agatttgttt gatgtttaat tataaataag
    16441 gttgtagata aaaaaataag taaagacaaa ggtttaaaat acttgcttta aatatagttt
    16501 gttttgaaca cttgccaaat taaaataatt aataaaatag acatcttctc atagtaaaat
    16561 atactgtgag aggatgtttt tgctttgttc ataaactttt atgaaaaatt tgcatagtgt
    16621 cacattttaa tatactatat atataataaa atatagataa aattaaatga ttcaaaagag
    16681 aaaggtgaaa agagatgtat tatataggtg tagacatagg aggaacaggt atacaagcag
    16741 gagtagttga taactatggg aagataattt ttaggtcaga gtgtaaaaca gtaattgaaa
    16801 aaggatttga aggaatactt aatgacataa aaataatgat ttataagttg ttggaagata
    16861 ataaacttac tatgagtgac ataaaatcta ttggatttgg tgttccaggt tttataaata
    16921 aagaaggatt agtaacttgt gtaaatttaa aatggaataa aaaagctttt aataaagagt
    16981 taaaaaaaag atttccagat gttgaaattc atggagaaaa tgatgcaaca gtagcagcac
    17041 ttggagaagc aaagtttgga agtatgaaag gtgctaatgt tggagtatta tatactttag
    17101 gaactggtgt aggtggagga atagtaatta atcaaaaagt cttttctgga gcacatggat
    17161 tgggttcaga aataggtcat caaattatag gagaaaacta ttttaattgt aattgtggaa
    17221 ataatggttg tgtagaaact ttttgttcag caactgcaat tataaagtat tctcaaaaac
    17281 tgatagaaga aggagaaaag agtaggattt tagatttggc agagggaaat ttagaaaatg
    17341 taaatgcaaa gatggtgttt gatgcatata gagaaaatga tttagtggca attaaagtaa
    17401 taaatagatt taaagagtat ttagcaaaaa cttttgcaaa cacaataaat tctttagacc
    17461 cagaaattat atctatagga ggaggcattt ctaaatctag cgatataata cttgatggaa
    17521 tagaaggttt ggtaagaaaa tttgtattat acaaaacaga agatatagct acaattacat
    17581 gtgcaacttt aggttcagac gcaggaatta taggagcagc ttttttataa tgtaaatgaa
    17641 ctgcttattg gaggcgtgat gatatatggg taaaacaaat gctagaagaa cgcttttttt
    17701 gatagcaata ggttcaggaa ctatgctgaa tcctcttaat tcttctatga tttcacttgc
    17761 tttacatagt atacaaaatg attttcatat tagttttaca actgtatctt ggattgtttc
    17821 agcattttac ctagctagtg ctattgcaca accagtttca ggtaagatag gagatttaat
    17881 aggtagaaag gtcttatttt tgagtggttt gatgcttgta attttatcag catccatagt
    17941 accacttgtt caatcattct ttatattgat ttttataaga gtaattcaag caattggaac
    18001 aagtacccta tatccatctg gagttgctct tatacaaaat aatataaaag aaagacaatc
    18061 ttctgcatta gcagttctaa ccatttttgc ttcaactaca gcaggattag gtccgacact
    18121 tggaggatta ttgttggact taggaggatg gcatgctata tttcttgtaa atatacctgt
    18181 tgttttggtt tctatatgct tagggtattt tttattccca aaagaagtaa aagaaaagaa
    18241 atctataaaa gaaactttaa aaaatgtttt gtcaagatta gatattattg gcatacttct
    18301 attttcaatt gcaatgattt ttatattact atttttgcta tctatgaagg aatcttttaa
    18361 cttagaacaa cttgtatttg gtataatact gatgtgtctt tttatttggc atgaattgag
    18421 aacaaaattg ccatttatag atattaagct ttttgtaagt aatcctaaat tgtcaaaagt
    18481 ttacttgcaa tttattatat taaatttctt taattatatt ttgttttttg gtcttcctag
    18541 ttattttcaa gatgccttac attatagtgc aaagtcgact ggattattta tgctatttat
    18601 gtctggtata ggtatattta tatctccatt aactggaaaa tggatagata aatctggaac
    18661 tagatttcct cttgttacat catcaatctt tatgtttatt agtgcagtac ttctttcagt
    18721 agtttttatc catatacctg taataggaaa gggtattata ttatcattag caggtattag
    18781 ttatggagtt ggtaatgtag ccctgcaatc ctctatgttt gaagaaagtc ctcgtgacag
    18841 tatagggaca gcttctggtc tgtttcaaac tagtcgttat ttaggttcta ttttagcaac
    18901 tgttttactt ggaatggtat ttgaagtaaa tataacatca gaacaatttc agatattagg
    18961 ttatgttatg gtggtcttag gtacaataag ttttttatta aattttatat taaagaaaga
    19021 attaagaaaa gtagaatagt ggatttatgg atttaataag tctaataact gataagttta
    19081 aaaatattgt ttgtaaaaca taactggttt gatatttatt aattctaagt tttgaagcat
    19141 agagtactcg ataagtatac aatattttga attaaaatta agcagaaatt tgaactagtc
    19201 tttcaaatct ctgcttaatt tagtagttaa atttttttat agagaaaata agatttgaca
    19261 cttagataaa aaatcattgc attttcaaaa ttctttaaat caaacccaag tatctcatta
    19321 atcttttcca atctatatac tagcgtgttt ctatgtaaaa acatactagt agatgtctta
    19381 gaaatattta aatcattatt tataaatttt attcctgtat atagaagttc ttcgtcaata
    19441 gatttaatct tattaaaatc tattaattta ttgagtatat tatcctcatt aattaaatct
    19501 aaagttttat atctaataag gtcaaacaaa tttaaaaact tattttcagc gttgtctttt
    19561 ataatatgag gtagtaaatc agaacataac tgtaatctaa aatcaaaatc agctttacta
    19621 tctaattcac caagataaaa aatataagta ctttgataag tttcagtttc aattatattt
    19681 tgtgtagtta cattaatatc taagttatat ttagaattta ctataaaaaa accattgtgt
    19741 tgtattatat ttatatcttc aaataaatta taaaaaatat tttttagata ttcaaagtct
    19801 atatgagatt ggtaaattaa ataaaagttg ccttttaaat tatcgagatt atttttatct
    19861 agaaaatcaa taacataaat agcattattg aaagtaacta aatggctact agcatctaac
    19921 ttttggtttt catatggata gatatttatt tttttattaa gctgtctttc taaaaaatct
    19981 tttaactctt ccataaagtc tccttaaaaa taagattata ttcttatctt atagctaaag
    20041 tagtttcttt gtcaaatatg tgtactctac tattgtcaac tgaaagtgtt aagtcatcac
    20101 caacattaat tctgttttta ctgtcaaatc taattgttat cgtattttcg cctagtttta
    20161 agtgagcata agtttctgct cccataagtt cttttatttc aattcttgat ttaaaacaag
    20221 tatctggatt agatttaata aaagtatctt ctacatgaat atcttctggt ctgataccta
    20281 taacaacttc attattaata taattgtctt ttattaaatc atagtgtttt tctttattca
    20341 atttaagctt aatatgttca ttttgtgcat aaatttcatc attttcttct attaatatta
    20401 catcaataaa attcatctgt ggtgctccaa taaatcctgc aacaaatata ttagctggat
    20461 aatcgtatac atcttgtgga gttgcaatct gctgaactac tccatccttc attactacga
    20521 ttctatcagc catagtcata gcttcaactt ggtcgtgtgt tacatagata aatgtagttc
    20581 caagctgttg gtgtagctta gttatctctg ttctcatagc agttctaagt tttgcatcta
    20641 agttggatag aggttcatcc attaagaata ccttaggatt tctaactatt gcacgtccta
    20701 aggcaaccct ttgtctttgt cctccagata atgttttagg ttttttattt aaaagaggta
    20761 gtaagtctaa tattttagca gcttctttaa cttttttgtc tatttcatct ttaggcaatt
    20821 ttctaagttt aagtgcaaat gccatgtttt cgtaaacaga taagtgggga tataaagcat
    20881 agttttgaaa aaccatagct atatctctat cttttggttc tatatcattg acaagtttat
    20941 ctccaatgta tagttctcct tcagagattt cttcaagacc agcaatcatt ctaagtgtag
    21001 tcgatttacc acaaccagat ggtcctacta aaacgataaa ttctttatct ttaatgtcta
    21061 tatttatatt ttttactgca ttaaatccat ttgagtaaag ttttgatata ttttttaaaa
    21121 ttacttctga cataatatcc tccttgatat ttaaatttta attatatagt atcaa
//
LOCUS       DAMJWU010000058        21061 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMJWU010000058 DAMJWU010000000
VERSION     DAMJWU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.058"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..369
                     /gene="tuf"
                     /locus_tag="PC460_002339"
     CDS             <1..369
                     /gene="tuf"
                     /locus_tag="PC460_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2601220.1"
                     /translation="DNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEE
                     GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLR
                     FSIREGGRTVASGVVATIIE"
     gene            741..1052
                     /gene="rpsJ"
                     /locus_tag="PC460_002340"
     CDS             741..1052
                     /gene="rpsJ"
                     /locus_tag="PC460_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF2601221.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            1144..1773
                     /gene="rplC"
                     /locus_tag="PC460_002341"
     CDS             1144..1773
                     /gene="rplC"
                     /locus_tag="PC460_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF2601222.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1803..2426
                     /gene="rplD"
                     /locus_tag="PC460_002342"
     CDS             1803..2426
                     /gene="rplD"
                     /locus_tag="PC460_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF2601223.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2426..2716
                     /gene="rplW"
                     /locus_tag="PC460_002343"
     CDS             2426..2716
                     /gene="rplW"
                     /locus_tag="PC460_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF2601224.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2746..3576
                     /gene="rplB"
                     /locus_tag="PC460_002344"
     CDS             2746..3576
                     /gene="rplB"
                     /locus_tag="PC460_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF2601225.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3611..3892
                     /gene="rpsS"
                     /locus_tag="PC460_002345"
     CDS             3611..3892
                     /gene="rpsS"
                     /locus_tag="PC460_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF2601226.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3924..4259
                     /gene="rplV"
                     /locus_tag="PC460_002346"
     CDS             3924..4259
                     /gene="rplV"
                     /locus_tag="PC460_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF2601227.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            4282..5097
                     /gene="rpsC"
                     /locus_tag="PC460_002347"
     CDS             4282..5097
                     /gene="rpsC"
                     /locus_tag="PC460_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF2601228.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            5133..5564
                     /gene="rplP"
                     /locus_tag="PC460_002348"
     CDS             5133..5564
                     /gene="rplP"
                     /locus_tag="PC460_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF2601229.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5566..5769
                     /gene="rpmC"
                     /locus_tag="PC460_002349"
     CDS             5566..5769
                     /gene="rpmC"
                     /locus_tag="PC460_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF2601230.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5793..6047
                     /gene="rpsQ"
                     /locus_tag="PC460_002350"
     CDS             5793..6047
                     /gene="rpsQ"
                     /locus_tag="PC460_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF2601231.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            6073..6441
                     /gene="rplN"
                     /locus_tag="PC460_002351"
     CDS             6073..6441
                     /gene="rplN"
                     /locus_tag="PC460_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF2601232.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6465..6770
                     /gene="rplX"
                     /locus_tag="PC460_002352"
     CDS             6465..6770
                     /gene="rplX"
                     /locus_tag="PC460_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF2601233.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6800..7342
                     /gene="rplE"
                     /locus_tag="PC460_002353"
     CDS             6800..7342
                     /gene="rplE"
                     /locus_tag="PC460_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF2601234.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            7360..7545
                     /locus_tag="PC460_002354"
     CDS             7360..7545
                     /locus_tag="PC460_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF2601235.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7578..7976
                     /gene="rpsH"
                     /locus_tag="PC460_002355"
     CDS             7578..7976
                     /gene="rpsH"
                     /locus_tag="PC460_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF2601236.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            8007..8549
                     /gene="rplF"
                     /locus_tag="PC460_002356"
     CDS             8007..8549
                     /gene="rplF"
                     /locus_tag="PC460_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF2601237.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8585..8953
                     /gene="rplR"
                     /locus_tag="PC460_002357"
     CDS             8585..8953
                     /gene="rplR"
                     /locus_tag="PC460_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF2601238.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8974..9483
                     /gene="rpsE"
                     /locus_tag="PC460_002358"
     CDS             8974..9483
                     /gene="rpsE"
                     /locus_tag="PC460_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF2601239.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9498..9683
                     /gene="rpmD"
                     /locus_tag="PC460_002359"
     CDS             9498..9683
                     /gene="rpmD"
                     /locus_tag="PC460_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF2601240.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9716..10159
                     /gene="rplO"
                     /locus_tag="PC460_002360"
     CDS             9716..10159
                     /gene="rplO"
                     /locus_tag="PC460_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF2601241.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            10203..11471
                     /gene="secY"
                     /locus_tag="PC460_002361"
     CDS             10203..11471
                     /gene="secY"
                     /locus_tag="PC460_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF2601242.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11488..12138
                     /locus_tag="PC460_002362"
     CDS             11488..12138
                     /locus_tag="PC460_002362"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF2601243.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            12139..12885
                     /gene="map"
                     /locus_tag="PC460_002363"
     CDS             12139..12885
                     /gene="map"
                     /locus_tag="PC460_002363"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF2601244.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12907..13176
                     /locus_tag="PC460_002364"
     CDS             12907..13176
                     /locus_tag="PC460_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF2601245.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            13214..13432
                     /gene="infA"
                     /locus_tag="PC460_002365"
     CDS             13214..13432
                     /gene="infA"
                     /locus_tag="PC460_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF2601246.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13468..13581
                     /gene="rpmJ"
                     /locus_tag="PC460_002366"
     CDS             13468..13581
                     /gene="rpmJ"
                     /locus_tag="PC460_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF2601247.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13734..14105
                     /gene="rpsM"
                     /locus_tag="PC460_002367"
     CDS             13734..14105
                     /gene="rpsM"
                     /locus_tag="PC460_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF2601248.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            14141..14539
                     /gene="rpsK"
                     /locus_tag="PC460_002368"
     CDS             14141..14539
                     /gene="rpsK"
                     /locus_tag="PC460_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF2601249.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14571..15194
                     /gene="rpsD"
                     /locus_tag="PC460_002369"
     CDS             14571..15194
                     /gene="rpsD"
                     /locus_tag="PC460_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF2601250.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            15272..16219
                     /locus_tag="PC460_002370"
     CDS             15272..16219
                     /locus_tag="PC460_002370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF2601251.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            16240..16581
                     /gene="rplQ"
                     /locus_tag="PC460_002371"
     CDS             16240..16581
                     /gene="rplQ"
                     /locus_tag="PC460_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF2601252.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16701..17534
                     /locus_tag="PC460_002372"
     CDS             16701..17534
                     /locus_tag="PC460_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2601253.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17522..18388
                     /locus_tag="PC460_002373"
     CDS             17522..18388
                     /locus_tag="PC460_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2601254.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18382..19185
                     /locus_tag="PC460_002374"
     CDS             18382..19185
                     /locus_tag="PC460_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF2601255.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            19217..19948
                     /gene="truA"
                     /locus_tag="PC460_002375"
     CDS             19217..19948
                     /gene="truA"
                     /locus_tag="PC460_002375"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2601256.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            20068..20499
                     /gene="rplM"
                     /locus_tag="PC460_002376"
     CDS             20068..20499
                     /gene="rplM"
                     /locus_tag="PC460_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF2601257.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20528..20920
                     /gene="rpsI"
                     /locus_tag="PC460_002377"
     CDS             20528..20920
                     /gene="rpsI"
                     /locus_tag="PC460_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF2601258.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8419 a         2489 c         4407 g         5746 t
ORIGIN      
        1 gataatatag gagcattatt aagaggagta caaagaaacg agatagaaag aggacaagta
       61 ctagcaaaga ctggatcagt aaaggcacac acaaagttta cagcagaagt atatgtactt
      121 aaaaaagaag aaggtggaag acatacacca ttctttgatg gatatagacc acaattctat
      181 ttcagaacaa cagacgtaac aggagcttgt aagttaccag aaggaataga gatggtaatg
      241 cctggagata acgtaacaat ggaagtagac ttaataaact caatagttgt agaagaggga
      301 ttaagattct caataagaga aggtggaaga acagtagctt caggagttgt tgctacaata
      361 atagagtaat cctttactaa actaatggat ttaatataaa aaaagagggg ggtaactcct
      421 ctcttttttg ttttttgttg acaagtagcg ctaaagcata tatactaaac tagtgccttg
      481 agacagatta acaatcaagt ttaaggttat gatgttgtaa tagaaaataa aattgaaaaa
      541 aacagaaaaa aaacttgaaa aaatgtaaca gatgatataa aatataatag tgtgcttgag
      601 agattaagaa aataataatt aaatcctagt gaaatacgaa ctgatatcga tttaaaagtt
      661 acaacacacc cggagcagtg tatcggtatt ggttgtacgc aacttgaaaa ttaaacacgt
      721 gataaaggag ggaaaacaaa atggctaaga atgaaaaaat aagaataaga ttaaaatcat
      781 atgatcacaa attattagat ttttcagcag gtaaaatagt tgaaactgct aaaaaggctg
      841 gatcacaagt ttcaggacct gtgcctctac caacagaaaa acaagttgta actatattaa
      901 gagctgtaca taagtacaaa gactctagag aacaatttga aataagaact cataaaagac
      961 taatcgacat agctaatcca acacctaaga cagttgactc attaatgaga ttagacttac
     1021 cagcaggtgt agatatagaa ataaaattat aagaacccct aagatattat cctatagatg
     1081 attatcttag aatgattact ctaaaagatg taatccgctg taagaattat aggaggtgct
     1141 aatatgaaag gaatattagg caaaaaagtt ggtatgactc aaatattcac tgataaaggt
     1201 gtagttatac ctgtaacagc tgttgaagca gggcctatgg tcgttactca aataaaaaca
     1261 gttgataaag atggatacaa tgctatacaa ataggttttg aagatgctaa agaaaaagct
     1321 ttaaacaaac ctaaaaaagg acatttagct gctgctaatg ttttaaagaa acatttaaaa
     1381 gaatttagag tagattctgt agaaggatac acagtaggac aagaaataaa agctgatgta
     1441 tttgaagcag gtgcaaaaat agatgttact ggaataagta aaggtaaggg attccaaggt
     1501 ccaataaaaa gacatggaca aagtagaggt cctgaaactc acggttctag ataccacaga
     1561 agaccaggtt caatgggagc ttgttcttac ccaggtagag tatttaaaaa caaaaaatta
     1621 gctggacata tgggaagcgt aaaggtaaca gttcaaaact tagaggttgt taaagtagat
     1681 gctgacaaga accttatatt agttaaagga gctataccag gagctaaagg ttcagtagta
     1741 actataaaag aagctataaa ggtttctaaa taatgactaa cctagagaaa ggaggaataa
     1801 caatgccaaa attaaatgta ctaaatgtta gtggacaaaa cgttggagaa atagaattat
     1861 cagattctat atttggcgta gaagttaatg gacatgtttt atacgaagtt gtaaaaaatc
     1921 aattagcaaa taagagacaa ggaactcaat ctgctaaaac tagagcagaa gttagaggtg
     1981 gcggaagaaa accttggaaa caaaaaggaa caggtagagc aagacaaggt tctacaagat
     2041 ctgtacaatg ggtaggtgga ggagttgctt ttgcacctaa accaagaagc tacaaatata
     2101 cattacctaa gaaggtaaga agattagcta tgaagagtgc tttatcttca aaagttcaaa
     2161 atagtgaagt tatagtatta gatgcattaa acatggatgc tcctaagact aaagaatttg
     2221 ctcaaatatt aaacaatata aatgctgcta aaaaagcttt agtagttata gctgacaaaa
     2281 acgataacgt aataaaatca gctagaaata tagaaggtgt tcaaactgca ttagttaata
     2341 ctatgaatgt atatgatata ttaaaatacg attcatttat aataactaca gatgctgtta
     2401 aaaaagtgga ggaggtgtac gcataatgac taatccacat gatgtaataa taagaccagt
     2461 tgtaactgag cacagtatgg ctgaaatggg tgaaaaaaaa tacacttttg ttgtggctaa
     2521 agatgcgaac aaaactgaaa taaaaaaagc agtagaaaaa gtattcggag ttagtgttga
     2581 taaagtaaat actttaaact acgatggaaa agttaaaaga atgggtagaa ctcaaggaag
     2641 aacatcaagc tttaagaaag cagtagttaa attaactgct gatagtaaag aaatagaatt
     2701 cttccaagga atgtagtatt ataacaaacg aaggagggaa aacagatggc tataaaaaag
     2761 tttagaccaa cttctcctgc cttaagacaa atgacagttt tagtttctga tgaaataaca
     2821 tgtaatcaac cagaaaaatc tcttttagtt aacttaaaga aaaatgctgg tagaaacgta
     2881 catggtagaa taactgttcg tcatagaggt ggaggacaaa aaagaaaata tagaataata
     2941 gattttaaaa gagataaaga tggtatacct gctaaggttg ctactataga atatgatcca
     3001 aacagaactg caaatatcgc attattaaac tatgctgatg gagaaaaaag atatatatta
     3061 gctccagttg gaattaatgt tggagatact atattatcag gattaggtgc tgatataaaa
     3121 ccaggaaact gtttagcatt aaaagatatg ccagttggta ctatcattca taatatagaa
     3181 ttaaagccag gaaaaggagc tcaattagtt agatctgctg gagtttctgc acaattaatg
     3241 gctaaagaag gaaaaaatgc tttattaaga ttaccatcag gtgagatgag attagtaagc
     3301 ataaactgta aagctactat aggtcaagtt ggaaatatag agcatggtaa cgtagttatc
     3361 ggtaaagcag gtagaaaaag acatatggga ataagaccta ctgtaagagg ttctgtaatg
     3421 aaccctaatg accatccaca cggtggaggg gaaggtagat ctccaatagg tagaccatct
     3481 cctgttactc catggggtaa accagctctt ggatacaaaa ctagaaagaa aaataaagct
     3541 tcaaataaac taatagtatc aagaagaact aagtagtaaa tttatttttg ctcggaaagg
     3601 aggaatataa atgtcaagat caactaaaaa agggcctttt gtccatgcaa gacttttaaa
     3661 gaagatagaa gctatgaatg ctagtggaaa taaagaagtt ataaaaactt ggtcaagatc
     3721 ttcaactgta tttccacaaa tggtagaaaa cactatagct gttcatgatg gaagaaaaca
     3781 tgttccagta tatataacag aagatatggt tggacataaa ttaggtgagt tcgttcctac
     3841 tagaactttc aaaggacata aggatgacga aaaatctaat aagagaaaat aattaaatta
     3901 tgactaagaa aggaggaata acaatggaag caaaagctac tgctaaatac gtacgtgtat
     3961 caccaagaaa agcaggccaa atatgtgacc ttgttagagg aaagaatgtt gatgaagcat
     4021 tagcaatatt aaagtttact ccaagaggag cagcttcaat aatagctaag gttgtaaaat
     4081 cagctaaagc taacgcagaa aacaatcacg aaatggatac tgaaaaatta tatatagcat
     4141 caatagttgc taatcaagga ccaacaatga agagattcat gcctagagct atgggtcgtg
     4201 caactacaat aagaaaaaga acttctcata tagaggttgt tgttaaagaa aaaaaataat
     4261 taatagatag gagggaaagt aatgggtcaa aaggttaatc cacacggctt aagggtcggt
     4321 gttataaaag attgggactc aagatggttc gctactgata aaaaagagtt cggaaactta
     4381 ttattagaag accataatat acgtaaattc ttaaagaaaa gattatactc agctggagtt
     4441 gctaagatag aaatagaaag atcagctaac aaaataaaaa tggacttaca cgttgctaag
     4501 cctggtgtag ttataggaag agctggtgca ggaatcgaag cattaaaagc tgaattagaa
     4561 aaaatgacaa agaaaactat aatagttaat atagtagaag taagaagtac agataaaaat
     4621 gctcaattag tagctgaaaa tatagcttta gctatagaga gaagggttgc tttcagaaga
     4681 gcaatgaaac aagctataca aagagcaatg aaatctggtg ctaaagggat aaaagtttct
     4741 gcttcaggaa gattaggtgg agctgaaatg gctagaactg aaggttatag tgaaggaaat
     4801 gtgccactac aaactttaag agcagatata gattatggtt tcgctgaagc tgatacaact
     4861 tatggaaaaa tcggtataaa agtttggata tgtaatggtg aagttttacc aactagagac
     4921 ggtgtaaatc caagagaaga aagcagaaag agcgatagaa gagacaacaa gagagataat
     4981 agaagaaacg atagaagagg aaatgacaga agaggaaatg ataacagagg aaactacaga
     5041 ggacaaagac ctcaaggtgg atcaagacct caaagaactg aaaacaaagg aaattaataa
     5101 tagttaggtt caaggaagga ggaaaaatac tcatgttaat gccaaaaaga gtaaagcgtc
     5161 gtagagttca tagaggaagc atggctgggc aagctcataa gggtaataaa gttacttatg
     5221 gtgagtttgg attagtagca ttagaagctt cttggataac ttctaatcaa atagaagctg
     5281 ccagaatcgc gatgactaga tatataaaaa gagggggaaa ggtttggata aaaatattcc
     5341 ctcataaacc agtaacaaga aaaccagcag agactcgtat gggtgcaggg aaaggttctc
     5401 cagaatattg ggtagctgta gttaagccag gaagagttat gtttgaatta gcaggtgttt
     5461 ctgaagataa agcaagagaa gctatgagac ttgctgcaca taaattacca atcaaatgta
     5521 aatttgttaa aaaagaagat ttagaagtaa agggtggtga ataggatgaa agctaaagaa
     5581 ttaagagatt taacaagcga agagctaatg aacaaattaa acgacttcaa aagtgaatta
     5641 tttagcttaa gattccaatt agctactggt caattagaaa atacagctag aataaagttt
     5701 gttaagaagg atatagcaaa agttaaaact gttcttgctg aaagaaagtt atacgaaact
     5761 agagcttaat ttggaaagga ggctttttaa acatggaaag aggaagaaga aaagttagaa
     5821 taggccgtgt tgttagtgat aagatggata aaacaatagt agtagctgtt gaagaattcg
     5881 tacgtcatcc actatataat aaacgtgtta agagaactaa gaaattcaag gctcatgatg
     5941 aaaagaatat atgtaacatc ggagatagag ttaaaataat ggaaactaga ccattatcta
     6001 aagataagag attcagacta gttgaagttg ttgagaaagt taagtagttt ttgaagaagg
     6061 agggattacg atatgataca acaagaatca cgtctaagag ttgctgataa ctcaggagct
     6121 aaagaacttt tatgtatacg tgttctaggc ggaagtaaaa gaagatatgg taacataggc
     6181 gacgttatag ttgctactgt taaaagtgca acacctggtg gagttgtaaa aaaaggtaag
     6241 gttgttaaag ctgttatagt aagaagcaaa caaggcgtaa gacgtaatga cggaagctat
     6301 atatcttttg atgaaaatgc tgctgttata ataaaggacg ataaaactcc agtaggaact
     6361 cgtatattcg ggcctgttgc tagagagtta agagacaatg aattcatgaa aatagtttct
     6421 cttgctccag aagtactata attaaggagg tgcaatagga catgatgcgt gttaaaaaag
     6481 gtgacactgt tgtagttata gcaggtaaag ataaaggtaa aaaaggttca gttttaaagg
     6541 tatatcctaa aactagcaaa gtgttagttg aaggtgtaaa tgtaataact aaacatcaaa
     6601 aaccaagtgc tatgaaccaa caaggtggaa taataaataa agaagcccca atacacatat
     6661 ctaatgtaat gccatttgat cctgaaacag gaaaaggcgt tagagtaaga tatgaagtaa
     6721 aagatgggaa caaagtaaga gtatcagcaa agagcggaaa agaattataa taaatattgc
     6781 ttgaaaggag ggaccttaaa tggcttctag attacaagaa aaatacatga aagaagttgc
     6841 tcctgcttta atggagaaat ttggatacaa aaacgtaatg gagataccta agttaaataa
     6901 aatagttatt aacatgggta taggtgacgc tagagaaaat ccaaaaggat tagaaaaagg
     6961 cgttgaagaa ttagaaatga tatcaggaca aaagcctgtt ataactaaag ctagaaaatc
     7021 tgttgctaac ttcaaattaa gagaaggaat gccaatagga acaaaagtta cattaagagc
     7081 tgacaaaatg ttctacttca tggacaaatt agtttcagtt tctttaccaa gagttagaga
     7141 ctttagagga gttaacccta atgcttttga tggtagagga aattatgctt taggagttaa
     7201 agaacaatta atattccctg aaatagaata tgataaaata gataaagtaa gaggaatgga
     7261 tataatattt gttactacag caaaaactga cgaagaagct cgtgaattat taaaattatt
     7321 aggaatgcca ttttctaagt aagcaaagga gggattcccg tggctagaaa agcgatggtt
     7381 gttaagcaac aaagaaagca aaagtacgct actagagaat acactagatg tactatatgc
     7441 ggaagaccgc attcagtttt gaaaaaattt ggtatatgcc gtatatgctt tagagaatta
     7501 gcttataaag gtcaaatacc tggtgttaga aaagcaagct ggtagaagct tgattagaat
     7561 ggaaggaggg caacaaaatg acaatgacag atccaattgc agatatgctt actcgtataa
     7621 gaaatgctaa tgttgttaag catgaaactg tagatgttcc agcttctaat atgaagaaag
     7681 aattagctag aatcttatta gaagaaggtt tcataagagg ttatgatgtt atagaagatg
     7741 gaaaacaagg tatcataaga atacaattaa agtacggaca agaaggcgaa agagttataa
     7801 caggtcttaa gaaaatatct aagcctggaa tgagagttta cgctgctaac catgagatac
     7861 caaaagtatt aaacggatta ggaatatcag ttatatctac ttcaaaagga atattaactg
     7921 acaagcaagc tagaaaagaa aatgttggtg gagaagtaat ctgctacgtt tggtaattaa
     7981 tatgaacaat gtaaggaggt gcgaccatgt caagaatagg tgttaagcca ataataatac
     8041 cagcaggtgt tgaagttact atagctgaag gaaatttagt tacagtaaaa ggtccaaaag
     8101 gaactttaac taaacaatta agtgctgagt taaacataaa aaaagaagaa aatacaataa
     8161 tggttgaaag accaactgat aataaaaaac atagatcttt acacggacta actagaactt
     8221 tattagataa tatggttgta ggtgtaaata ctggtttcga aaagaaatta gaattaaaag
     8281 gtgttggata tagagctcaa aaacaaggaa agaaattagt tatgaactta ggtttctctc
     8341 atccagttga gatggaagat ccagaaggaa taactgttga agctccaaac caaacagagt
     8401 taatagtaaa aggtatagac aagcaattag tagggaatta tgctgctaag ataagagctt
     8461 ggagaaagcc agagccatac aaaggtaaag gtataaaata cgttgatgaa gtaatcagac
     8521 gtaaagaagg aaaaactggt aaaaaataat accaaaactt aaacgctaga aaggagtgag
     8581 ctaggtgtta aaaaaggctg ataaaaatgc taatagactt caaagacata agagagttcg
     8641 tagaaaaata agcggtactt ctcaaagacc aagattatgt gtatttagaa gtgctaacaa
     8701 tatatatgct caaataatag atgacacaaa aagagtaact ctagttgctg catcttcttt
     8761 agaagcagaa gttaagagtg ctgtcaatca tacaggaaac aaggaagcag ctaagaaagt
     8821 tggagaatta gttgctaaaa aagctgttga aaaaggaatc actgaagttg tatttgacag
     8881 aggtggatac ttatatcatg gaagaattca agaattagct gaaggtgcta gagaagctgg
     8941 tcttaagttc taatacgaag gagggaaaaa tacatgctac gtcgtaagcc gatagatgca
     9001 ggacaacttg acttacaaga aaaagttgtt gaagtaagac gtgttactaa ggttgtaaaa
     9061 ggtggtagaa atttcagatt tgcagcatta gtagttgttg gagatgaaaa cggacacgtt
     9121 ggtataggtg ctgggaaagc aatggaagta ccagacgcta taaaaaaagc agttgaagat
     9181 gctaagaaaa atctaatagt agtgccaata gttgggacta caattcctca cgaagtacgc
     9241 ggacactttg gtgctggaaa tatattaata atgcctgctg tagaaggtac aggagtaata
     9301 gctgggggac ctgctagagc cgtacttgag ctagctggac taaaagacgt tagagctaaa
     9361 tctttagggt ctaacaatcc aagaaatatg gtaaatgcta caatagaagg acttaattct
     9421 ctaaaaacag ttgaagatat agctaaactt agaggtaaaa aagttgaaga acttctaggg
     9481 taaggaggta gtgtaaaatg gctaaattac aaataaagtt agttagaagt gttataggaa
     9541 ctactcctaa ccaaaaaaag aatgttgaag cgttaggatt aagaaaaaga gaacaagttg
     9601 ttgtaaaaga agacaatgct caaacaagag gtatgataaa taaagtaagt cacttattag
     9661 aagtaactga aatagctgaa taatttttat aaagtagtgc aaggaggtgc aatccatgaa
     9721 gttacatgag ttaaaacctg ctgaaggtgc agtaagagct aagagaagat taggtagagg
     9781 tactgcaact ggacaaggta aaactgcagg acgtggacaa aaaggtcaat ggtctcgttc
     9841 tggtggtgga gtaagagtag gatttgaagg tggacaaatg ccactagcta gaagacttcc
     9901 taagagaggt ttcaataaca tatttaagaa agtttacaca gaagttaatg ttgaagtttt
     9961 aaacagattt gaaaatggaa cagaaataac tgcagaatta ttaaaatcta ctaaaactat
    10021 aagcaaaata ggtaaagacg gaattaaaat cttaggtgaa ggtaatttag agaaagcttt
    10081 aactgttaaa gctgctaaat ttacagcttc agctcaagaa aaaatagaaa aagctggtgg
    10141 aaaagcagag ttagtataat aacctgctaa tctcctagcc agtacaggcg gggtgaatta
    10201 gcgtgctgtc aaaattaaaa caagcttgga agattaaagc agtaagaaaa aaagtaatgt
    10261 atacacttat gatgatcgtg atattcagaa taggtactac tataccagtt ccaggcatag
    10321 acactagtat tatacaaaag atggtaggcg gcaatagtct actttctctg tataatatgt
    10381 ttactggtgg agcatttagt aacttttcac tatttgcttt aggtataagt ccatatatca
    10441 ctgcatcaat cataatacaa cttctaacag taggttttga aagtttagca gaacttcaaa
    10501 aatctggtga agaaggaaag aaaaaaatca ataagtacac taaatataca gcactagctc
    10561 tagcagttgt acaagctttg ggaattacat taggaattgt aagaagtgct ttaatatcaa
    10621 atagtgtgtt ttttatcaca actgttgtaa taacattagt ttcagcaagt atgttagtaa
    10681 tgtggattgg ggacaaaatt actgaaaaag gaatcggtaa tggtagttca gtaataatat
    10741 ttgcaggtat aatatcaaga atacctacag atgttattaa aatatcgcaa caagttaaat
    10801 ctggcgaagt tgcaccttgg gttatagtga ttctagctgt agttatatta cttacagtaa
    10861 caggagttac atttatacaa gaagcaacta gaaagatacc tgtacaatat gctaaaagag
    10921 ttgttggaag aaaaatgtat ggaggacaaa gttctcatat accaatgaaa gttaaccagt
    10981 caggagttat gcctataatt tttgctagtt cacttttagc tttccctcaa acaatagcta
    11041 tgtttatggg tccaaatgct caggcttttg ttcaaaaata cttaagtatg gcaacagaac
    11101 aaggattttg gacatataga tcaattgaga ttctacttat aattttcttc tcatattttt
    11161 atacaactgt atcatttaat acagaagaca tatctaaaaa tatgaaaaat aatggtggat
    11221 ttataccagg tataagacca ggtgagccta ctatggatta tttaaataga attttaacta
    11281 gattaacttt agctggagct actttcttag caataattgc tatggtgcca gctcttacaa
    11341 cacattatat gaaagtaaat atgagtctag ctgggacttc attgctaatc gttgttggag
    11401 ttgctttgga gcttaagaga cagttagagt caaacttagt tatgagaagc tatcaaggtt
    11461 tcttaaaata aaatggagat ggataatatg agaataatat tacttggacc tccaggtgct
    11521 ggtaaaggta ctcaggcggc aggaatagta gaaaaataca atatacctca tatatcaaca
    11581 ggagatatat tcagaaagaa tataaaagag ggaacagaac ttggaaaaaa agctaaagaa
    11641 tacatggacc aaggtttatt agtaccagat gagttaactg taggtttagt tactgataga
    11701 atatctcaag aagattgtaa aaatggattt atgttagatg gatttccaag aaatgtagca
    11761 caaggagaac atttagatat cttcttaaaa aatgctggta tatcactaga taaagttgtc
    11821 aatattgaag ttgataagag tatattagtg tctagagcag ttggtagaag aatatgtaag
    11881 tcttgtggag ctacttacca tgttgagttt aatcctccta aagtagaagg tgtatgtgat
    11941 gtatgccaag gagaattata tcaaagagct gatgataatg aagaaactgt atctaagaga
    12001 atacaagttt atctagatga aactaagcct ttagtagatt attatagcaa acaaggtata
    12061 atagcagata taaaaggtga tcaagcaata gataaagtat ttgaagatat tgtcgcagcc
    12121 ttaggaagtg gaaaataaat gattatcttg aaatcaaaaa aagaaataga acttttaaga
    12181 gaagcaggta aaatagttgc tgacactcat gaagttttaa gaaaagctat ttctccggga
    12241 atatctactt tagaattaga taaaatagct gaagagaata taagaaaata caatgcagaa
    12301 ccttctttta aaggatatgg aggttttcca ggaagtatat gtgcttctat caatagagag
    12361 gtagtacatg gtattccagg agaaactata ttacaagaag gtgacattgt aagtttggat
    12421 ataggagcct attataaagg atatcatggt gattctgcga aaactcacgg agtaggcatg
    12481 atatctgaag aagataggaa attgatagaa gtaactagag aaagcttcta tgaaggaata
    12541 aaatttgcta aattaggata tagactttca gatatctcac acgcagtaca aacacacgtt
    12601 gagaaacatg gtttctcggt agttagagat ttagtgggtc atggagttgg agcaaatctt
    12661 catgaagacc cacaagtacc taattatgga ctcccaggaa aaggaccaag acttagagaa
    12721 ggaatggtta ttgctataga gccaatggtt aatgcaggtc gttatcatgt aaagactcta
    12781 tcagatgggt ggacgactgt tacaatagat ggaaaaaaat cagctcatta tgagcatacg
    12841 atagcaatta ctgaacatga acctttgata ttgacaaagt tgtaatttaa aaagtaggtg
    12901 aaaaaagtgc tatcagataa tttaagtata ggtcaagttg ttaaagtttc attaggacga
    12961 gataaaggaa atttattctt tgtagtcaaa ataattaata atgaatatgt attaatagct
    13021 gatggaaaaa agagaaaact tgacaaacct aagttaaaga aagtgaagca cttaaaaaaa
    13081 tataatttta ttaatgatga agttagaaaa agagtagtat ctggacaaga gattacagat
    13141 tcttttttaa gagctgaact tactaagtta aattagataa tttaacttat tgaaatggag
    13201 ggctggttag ttaatggcca aaaaagatgt tatagaatta gaaggtacag tttcagaagc
    13261 tttacctaat gctatgttca aagttaaact agaaaatgga catgagatat tatgccacat
    13321 ttctggaaag ctaagaatga acttcataag aattcttgaa ggtgacaagg tgaatgttga
    13381 actttctcca tatgacctta caagaggaag aattacttgg cgtaagaagt agactatatt
    13441 aatatttagt ctaaggaggg attaataatg aaggttagac catcagtaaa accaatatgt
    13501 gaaaaatgta aagtaataaa aagaaaagga aaagtaatgg ttatctgtga aaatccaaag
    13561 cataagcaaa agcaaggata agaaattatt actagcattt tgttaaattt tatagtataa
    13621 tactatattg tgatgttaac aaaagaacgt taaaatcgtt gaacaatctg gcgtaggatt
    13681 ggcatatgat gtgaagtttt aaacactaaa aactatgtgt aggaggtgcg atcatggcaa
    13741 gaatagctgg ggtagattta cctagagaaa aaagagcgga gataggctta acttatatat
    13801 atggtatagg taaagcaact gctaacgaaa tattagctaa ggctgagata aatccagata
    13861 ctagaataaa agatttatca gaagatcaag ttaatgagtt aagaaaagtt atagatgacg
    13921 atttcttagt tgaaggtgac ttaagaagag aaattgcttt aaacataaag agattaagag
    13981 atataaaatg ttatagagga ataagacacg ctaaaggtct tccattaaga ggacaaagaa
    14041 ctaagactaa tgctagaact agaaaaggtc ctagaaaaac agtatctcgt aaaaagaaaa
    14101 aataaataaa taataactag atgaaggagg gaaaaggaaa atggctaaac caaaaaagaa
    14161 agttacacgt attagaagaa gagaacgtaa aaatatagaa cgtggtcatg ctcatataca
    14221 atcaactttc aataatacaa taataacttt aactgacgtt cacggaaatg ctatatcttg
    14281 ggcaagttct ggacaattag gattcaaagg atcaagaaaa tcaactccat ttgcatctca
    14341 aatggctgct gaaacagctg caaaagctgc gatggaacac ggactaaaaa gtgttgaggt
    14401 attcgtaaag gggccaggtt caggaagaga agctgcaata agagctttac aagcaactgg
    14461 actagaagta actatgataa aagacgttac tccaatccca cataacggat gcagaccacc
    14521 aaagagaaga agagtgtaat taataagagt acaataggag gtgtagataa atggcaagat
    14581 atacaggtgc atcatgtaga caatgccgta gagagggaat gaaattattc cttaaaggtg
    14641 atagatgtta tacagacaaa tgtgctatag taaaaagaaa ctatgctcca ggtcaacatg
    14701 gccaaggaag aaagaaagtt tctaactatg gattacaatt aagagaaaaa caaaaagtta
    14761 aaagaatata tggagtttta gagactcaat ttagaaattt atatgaacgt gctgaaaata
    14821 tgcctggtaa agcaggtgag aacttattaa gtttattaga gagaagatta gataatgtag
    14881 tttacagaat gggactagca tcttctagaa aagaagctag acaattagtg actcatggtc
    14941 atttcacttt aaatggaaat aaagtagaca taccttcatt aatagttaag gttggagatg
    15001 ttatagaagt taaagagaaa tcaagatctt ctgcaaaatt caaaaattta gtagaagtta
    15061 actcaagaat agctcctaag tggttagaag ctaatgtaga aggaatgaca gcaaaagtag
    15121 ttggtgttcc aacaagagaa gatatagatc ttgagatagc agagcactta atcatagaac
    15181 tttactctaa ataataagca cacttttaaa aattatttta aaattttgtt taccctcagt
    15241 ggattactga attttaagga gggttttgtc catgatagaa atagaaaagc caaaagtaga
    15301 tatagttgag cttagcgaag actatagata tggtaagttt gttatagagc ctctagaaag
    15361 aggatatgga ataactatag gtaacgcatt aagaagaata ttattatcat cattaccagg
    15421 tgtagcagtg aatgctataa aaatagatgg agttcttcat gaattttcaa caatacctgg
    15481 tgttaaagaa gatgttactg agataatatt aactttaaaa gagctttcag caactataga
    15541 tggtgaagga agtagaacac ttaaaataga agctcaagga ccatgctcta tcacaggtgc
    15601 agatataatc tgtcctccag atgttgaaat attaagtaaa gatttagcaa tagctacatt
    15661 agatgataat gctaaactta atatggagat atttgtagat aaaggtagag gttatgtttc
    15721 tgctgaagaa aataaaacag agaatgttcc aataggtgtt ttacctgtgg attcaatata
    15781 tactcctgtt gagaaggtta gttatcatgt ggaaaacaca agagttggtc aaaaaacaga
    15841 ttatgataaa ttagtattag aagtttggac taatggtagt ataaatcctc aagagggaat
    15901 atcattagct gcaaaggtat tagttgagca tttaaactta tttatagact taactgaaca
    15961 tgtaagtagt gttgaaatca tggtagaaaa agaagaagat caaaaagaaa aagttcttga
    16021 aatgactata gaagaattag atttatcagt tagatcatat aactgtttaa agagagcggg
    16081 aattaataca gttgaagaat tagctaataa atctgaggat gatatgatga aggttagaaa
    16141 cctaggtaag aagtcattag aagaggtaat acaaaaacta gaagaacttg gattgggatt
    16201 aaaaccaagt gaagaatagc gagcaaagga gggataagaa tggctaagta ccgtaaatta
    16261 ggacgtgaaa cagctcacag aaaccttatg ttaagaaact tagtaacttg cttactaaga
    16321 agtggaagaa tagaaactac agtgactaga gcgaaagaaa ctcgtagaat ggctgaaaag
    16381 atgataactc ttgctaagag aggagatctt catgctagaa gacaagtttt agcttatgtt
    16441 atggatgaaa cagtagttaa taacttattc acagatttag ctccaaaata tgctgagaga
    16501 aatggtggat acactagaat aataaaaata gggccaagaa aaggcgatgc tgctgaaatg
    16561 gcttttatag aattagtata gtaaggaaag gattaggctt gatggcttaa tctttttttt
    16621 atttttttga ctaaggtatg atataataat gtgtaaaatg atttttataa gttattgctt
    16681 actaaaagga gtatgagtta atggataata tagtaaaggt aaataatatt tcgtttgagt
    16741 atattacaga tgaagcaaaa cttaaagcaa tagataattt aagcctagat gttaaaaaag
    16801 gagaatttgt tgcgataata ggacataatg gttctggtaa gtctacttta tctaaaaact
    16861 taaatgctat tcttatgcct actgaaggaa atattcttat tgatgatatg gatactaaag
    16921 aagaagaaag attatgggat ataagacaaa ctgcaggcat ggtatttcaa aatccagata
    16981 atcaaatagt tgctactata gtagaagaag acgttgcatt tggacctgag aacttaggta
    17041 tagaaccaaa agaaataaga cgaattgtag aggagtcttt aaaaagtgta ggtatgtatg
    17101 atttaagaga tagacagcct catttgttat caggaggtca aaagcaaaga gttgcaatag
    17161 caggaattat tgctatgagg cctaaatgta taatatttga tgaagcaaca gcaatgttag
    17221 atccatctgg aagaaaagaa gttatgaaaa caataaaaag acttaataag gaagaaaata
    17281 taactgttat acatataacg cactttatgg aagaagcagt agaagctgat agagttgtag
    17341 ttatggaaaa aggaaagaaa atcttagagg gaactcctag agaagtattc agtaaaataa
    17401 agatgttaaa ggaaatcggt ttagatgtac cttgtatgac agagttatct agtttattaa
    17461 tagaagaagg tataaatatt agtagtgata tattaactgt ggatgagatg gtgatggaat
    17521 tatgtcaatt atagtaaaga atttaacaca tatatataat gaagggatgc cttttgcaag
    17581 taaggcactt gatgatgttt cttttgagat taaagataga gattttgttg gtcttatagg
    17641 tcatacaggc tcaggaaaat ctacattaat acaacattta aatggattac taaagccttc
    17701 ctcaggagaa atatttataa atgattttaa tataacagat aaaaacttga atttaactga
    17761 gattagaaaa agagtagggg ttgtatttca atatccagaa taccaattgt ttgaagaaac
    17821 gattgataaa gatatagctt ttggaccctc taatttaggc ttagaggagt ctgagataca
    17881 taacagggta aaagcatcaa tggaagctgt aggacttgat tatgagggat ttaaagacaa
    17941 atctcctttt gaattatctg ggggacaaaa gcgtagagta gcaattgctg gagttatagc
    18001 catgaaccca gaagttctca tattggatga accaactgct ggtcttgacc ctggtggaag
    18061 agatgaaatt tttaacttaa taaaagactt acatgaaaaa aagaatatga ctataatact
    18121 atcatcacat agtatggatg atatggcaaa gttagcaaaa actttaattg ttatgaatca
    18181 tggtagcgta gaatttatgg gaactcctag agaagtgttt aaatcaaatg ctagtaaatt
    18241 aaaagatata ggattagata ttccacaagt tttagaactt gcattgaaat tgagagaaaa
    18301 aggctttgac atcagtgagg atatattgac tttagaagaa gcaaaacagg aaatattaaa
    18361 agttgtgaga ggacgaggat tatgttaaaa gatataacta tagggcaata ctacccaaca
    18421 agttctgcta ttcataaatt agatccaagg ataaagcttg tagcgacaat tgtatttatg
    18481 gtatctatat ttgtagtaaa taaattttgg ccttatatag tagttttatt atgtttatta
    18541 gctatgataa aattggctaa tataccagtc aaatatattg ttaagggtgt taaacctctt
    18601 aaatggatta ttttgtttac atttcttata aatatttttt tcttaccagg tgatgaaata
    18661 tggtcatttg gatttttagc tattacaaaa cagggtctaa gacaagcaat ttttatggca
    18721 ataagattaa tatttttagt agttggtaca tcattgctta ctttaacaac ttcaccaata
    18781 gaattaactg atggaataga gagattgttg aatccattta agaaaattgg tttgccagtt
    18841 catgaacttg caatgatgat gacaattgct ttacgtttta ttcctacatt gttagatgag
    18901 acagacaaaa taatgaaggc tcagatgtct agaggtgcag attttgaaag taagaatctt
    18961 ataaatagag caaagaattt agtaccactt ttggtacctt tatttgtaag tgcatttaga
    19021 agagctgatg aattggctat ggccatggag gccagatgtt acagaggtgg acataatagg
    19081 actaagatga gagagtctgt tatatcaaga agggattata tggcatgtgt atttcaggtt
    19141 gtttatctgg gagcaattat agctactaga tttatagcaa tttaattaat aagttataga
    19201 tataatgagg taaagtatga gaaatataaa aattaaaatc caatacaatg gtaagaatta
    19261 ttgtggatgg caaaagcaac ctgattcttt agggatacaa ggtacaattg aaagggctat
    19321 atatgatata acaaaagaag aaaccagtct tatagggtct ggtagaactg attcaggagt
    19381 tcatgctatt ggtcagattg caaattttaa aattaattca ggtataagta tagaaagtat
    19441 accaatggca ctaaatgcca agttgccaaa agacatatca gttatagaag catgtgaagt
    19501 aaatgatgat tttcactcca gatacagtgc aaaaggtaaa acatataaat atttagtata
    19561 taatagcaag tttagaaatc caatacttag tgagatatca tatcaagtaa aatatgaact
    19621 tgattttgat aaaatgtgtt cagaagcaaa aagtttatta ggaacacatg attttaaagg
    19681 ttttatgagt tctggttcat ctgtaaaaga tacagttaga acgatatatg atattgatat
    19741 aagtaaaaaa gatgacttga ttacttttga gattagtggt aatggttttt tatataacat
    19801 ggtgaggatt attgttggaa cacttgttga tatgggtcgt ggcagaatta acgaaccatt
    19861 tttagatata atacaatcaa aaactaggtc aagatgtgga catacagctc cagcacaagg
    19921 attatttctc aaaaaagttc attattaact tgacacacca ggctacatgt attaaaatat
    19981 atgagaatgt gttaataagt ccacttgccc cggacttgac ataaacggta aaaaaatttt
    20041 atgaaaagta gttaaggagg gacagttatg aaaagttata tagctaagcc agctgatgta
    20101 caaagaaaat ggtacttagt tgatgctgaa ggaaaaacat taggtcgttt agcaacagaa
    20161 attgcgacag tattgagagg taaacataaa ccaacattta ctcctcacgt tgatggtgga
    20221 gattttgttg ttgtagtaaa tgcagaaaaa atagttttaa gtggtaagaa attagatcaa
    20281 aaatattaca gataccatac tggttatgta ggtggattaa aagaaatatc ttacagagat
    20341 atgatggata agaaaccaga agaagttata tcacatgctg taagcggtat gttacctaaa
    20401 aataaattaa gaagcagaat gatgactaga ttaagagtat ttgcaggtgc tgagcacact
    20461 catgcagctc aaaatccaga agttttaaac tttaaataag attaataggt gaaaggagga
    20521 gtttataatg gctaacgttc aatattatgg aactggaaga agaaaaagtt ctgttgctag
    20581 agtaagacta gttgcaggtg aaggaaatat attagtaaat ggaagagcat tagaaaatta
    20641 ttttaattat gagacattaa taagagatgt taaacaacca ttagttttaa ctggtaatga
    20701 aaataaatat gatgttatag taaaagttga aggtggagga ttcactggac aagctggagc
    20761 tataagacat ggaatatcta gagctttatt aaaagctgat ttagatttaa gacctgcttt
    20821 aaagaaagaa ggtttcttaa ctagagatgc aagaatgaag gaaagaaaga aatacggatt
    20881 aaaagcagca agaagagctc cacaattctc aaaaagataa ttacttattt ttttggaaaa
    20941 gcaaaatcct ttatttactg ttattcagaa agtattggta ttttaaaaat gttgggttaa
    21001 gttaattatt gatatataat aaaataataa gatatcttat taggatgttt ttcattaaga
    21061 a
//
LOCUS       DAMJWU010000059        20998 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMJWU010000059 DAMJWU010000000
VERSION     DAMJWU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.059"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            195..1256
                     /locus_tag="PC460_002378"
     CDS             195..1256
                     /locus_tag="PC460_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2601259.1"
                     /translation="MRDFLKNYKSSIILLACTIVGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKGTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKNLMVTQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     IGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIFMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1269..1466
                     /locus_tag="PC460_002379"
     CDS             1269..1466
                     /locus_tag="PC460_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2601260.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1630..2334)
                     /locus_tag="PC460_002380"
     CDS             complement(1630..2334)
                     /locus_tag="PC460_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2601261.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYTKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            2499..3224
                     /locus_tag="PC460_002381"
     CDS             2499..3224
                     /locus_tag="PC460_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2601262.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSMSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3284..4876
                     /gene="hcp"
                     /locus_tag="PC460_002382"
                     /gene_synonym="priS"
     CDS             3284..4876
                     /gene="hcp"
                     /locus_tag="PC460_002382"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF2601263.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDICGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5248..6153
                     /locus_tag="PC460_002383"
     CDS             5248..6153
                     /locus_tag="PC460_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2601264.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6277..7230
                     /gene="msrA"
                     /locus_tag="PC460_002384"
     CDS             6277..7230
                     /gene="msrA"
                     /locus_tag="PC460_002384"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF2601265.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTHIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            7383..8513
                     /locus_tag="PC460_002385"
     CDS             7383..8513
                     /locus_tag="PC460_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601266.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8630..9598)
                     /locus_tag="PC460_002386"
     CDS             complement(8630..9598)
                     /locus_tag="PC460_002386"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF2601267.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            9933..10121
                     /locus_tag="PC460_002387"
     CDS             9933..10121
                     /locus_tag="PC460_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601268.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            10381..11580
                     /locus_tag="PC460_002388"
     CDS             10381..11580
                     /locus_tag="PC460_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF2601269.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIESDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11632..12135
                     /locus_tag="PC460_002389"
     CDS             11632..12135
                     /locus_tag="PC460_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2601270.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            12242..12559
                     /locus_tag="PC460_002390"
     CDS             12242..12559
                     /locus_tag="PC460_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601271.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            12717..13676
                     /locus_tag="PC460_002391"
     CDS             12717..13676
                     /locus_tag="PC460_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2601272.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            13791..14576
                     /locus_tag="PC460_002392"
     CDS             13791..14576
                     /locus_tag="PC460_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601273.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDKKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            14868..16187
                     /locus_tag="PC460_002393"
     CDS             14868..16187
                     /locus_tag="PC460_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2601274.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(16262..16690)
                     /locus_tag="PC460_002394"
     CDS             complement(16262..16690)
                     /locus_tag="PC460_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601275.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(16781..18157)
                     /gene="hydG"
                     /locus_tag="PC460_002395"
     CDS             complement(16781..18157)
                     /gene="hydG"
                     /locus_tag="PC460_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF2601276.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIREEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(18158..19243)
                     /gene="hydE"
                     /locus_tag="PC460_002396"
     CDS             complement(18158..19243)
                     /gene="hydE"
                     /locus_tag="PC460_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF2601277.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(19279..20487)
                     /gene="hydF"
                     /locus_tag="PC460_002397"
     CDS             complement(19279..20487)
                     /gene="hydF"
                     /locus_tag="PC460_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF2601278.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLIVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDSGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(20502..20753)
                     /locus_tag="PC460_002398"
     CDS             complement(20502..20753)
                     /locus_tag="PC460_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF2601279.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         7897 a         2483 c         3405 g         7213 t
ORIGIN      
        1 atatatagat atgaactatt aataaaacgt taaaaataca aaaaaactat agaaattgac
       61 atatattcca gtatttgcta aagtagctgt tggggaaaat tacaaaaatt attaatgcgc
      121 attagataat ttactttaat ttttttagca tttggggtaa tttataatat aaagcattag
      181 aaaggaaaat ataaatgagg gactttttaa aaaattataa gtcatcaatt atacttttag
      241 catgtactat agtaggggcg atagtaggat tagtacttgg tccaaaagct tcaatactaa
      301 aaccatttgg agatttattc ataaatgctc tattaatgat agtagtacca ttagtttttc
      361 ttagtgtttc ttcaggtgtt gctagtataa aaaatatgga acgatttgga aaaataatga
      421 agggtacagc tatagtattt atgggaacaa cacttgtaat tgcattttta ggtattacat
      481 ctgcattatt atttaatccg tctaaaggat tagatgtatc aagtgtaaaa aatttaatgg
      541 tgactcaaga tgcagaaact ttagaatcaa aaggaacgtt attagaccaa gtagtatcaa
      601 gtattacagt taaaaatttt ggagatttat tttccagtag taatttacta caattagtta
      661 tatttgcagt tttaattgga acagcaatag ctatgatggg agatagggga aatacaataa
      721 gaaagctttt atcagaagga acggaagcaa tattaaaagt agtaagtata ttcatgcttt
      781 atgctcctat agggctaggc tgttatacag ctacaataat tggggaactt ggaagtaaaa
      841 taattcaagg atatgtaagg gtgtttgtaa tttattcact aacagcagcc atgtatttct
      901 tattatttta cagtctgtat gcttttttat ctgcaggaaa acaagggttt agacaattct
      961 ggagaaatgc tataaaacca atagcaacgg cactagctac ttcttcaagt tcagcatgta
     1021 taccatcaaa tttagaagct agtaaaaata taggagttcc tgatgatata tcagaaacag
     1081 ttatacctct tggaataaat atacataaag atgggtcagt tttagcagta ataataaaag
     1141 tggcattttt atcaggtatt ttaggtagtg gaatggcaaa tccagcaacg ataggtcaat
     1201 tatttataac tgtatttgtt gttggagtat ttatggctcc tgtagcaggt ggctgactta
     1261 ttggtgagat gctcgtggtt agcatcttcg gttttccagt ggaggcaatt ccactgttaa
     1321 cagttataac tactataact gatattccta atactatatt aaatacaact ggaaatacag
     1381 tatcatcaat gttagtttca agattagtag aaggaaaaga ttggcttata gataaaacag
     1441 caattactac aaagaaaata agctaattgt acacttaatt aataaagtta ataggtatct
     1501 taattttaat aaattagata cctattaact ttttaagtaa aaatataaat ttaagattca
     1561 aatatgatat ttataatttg aatcaactaa tatagtatat attgaaataa atatgtttaa
     1621 atatctattt tattttttaa aataaaagat ttcaaattta tttttgttaa aagataacaa
     1681 gccttcatct cgcatcttgc caagctctct tgacatagaa cttctgtcaa cacataaaaa
     1741 atcagccatt tcatttcttg aaaagggaat tgtaaactgg tggctacctg tttgttctat
     1801 ctgttttgaa aaatacataa gcaatttctc gcgagttgtt ttacgagaaa gtaattctat
     1861 tttattgtga agtaaaatgt ttttttcagc tattaattgt agcatatttt caacaagcct
     1921 attatgaaat acacatgcag atgaacaaat accagttaca cttttaaact ttatgaataa
     1981 aacttcgcaa gaagatgttg tggttactgt aacattactt ttatgtagtc tagtacatgc
     2041 gaatacctct ccaaataaat ctccaggcaa gagttcagct acaatattcc tattcccttc
     2101 gatatcttcc tttattatat gagcacttcc tttaagcata attccaatag aagtaacttc
     2161 acttccaaca gagaatatta tactatcttt tgtgtaagtc ttatctacaa cacctaaaca
     2221 ttccaacatt tcttcaagtt catcactttt aattccttca aatagaggaa tttttttaag
     2281 taggtctata tgcttttcta aaaatttttt caaataatca cttccttgtt gcatatgcaa
     2341 ccgataattt gttttcattg tactatacta agtttataaa agcaagggga tataaaaatc
     2401 ctactacaag aaatttgaaa taaagaaata taggtattaa aaatatatat ataagaaatc
     2461 aatactttaa attaaaaaaa ttttatgagg tgatataaat gatgagaaaa ataattaaaa
     2521 ttgatgagga aaagtgtaat ggttgtggac tatgtgttga agcttgtcat gaagaagcta
     2581 taggaatggt aaatggaaaa gcaaaacttt taagagatga ctattgtgat gggctgggag
     2641 attgtctgcc aacttgtcca acaaatgcga tttcatttga gtatagagaa gcagcagaat
     2701 atgatgaagc tgcagtaaaa gcaaatatgg aagcaaagaa agcacaaaag aaaactttag
     2761 catgtggatg tccaggttct cagtctaagt caataaatag agaggtttca aatagtatgt
     2821 ctataagcaa tgatatagta gagataaaag gttctcaact taatcaatgg ccagtacaaa
     2881 taaaattagt accaactaat gcatcatatt taaagaatgc aagtctttta atagcagcag
     2941 actgtacagc gtatgcatat ggaaattttc ataataaatt tatgaaaaat aaggttacat
     3001 taatcggatg ccctaaactt gatgaagttg actatgctga aaaacttaca gagattttaa
     3061 aagaaaatga tattaaaaat atagtagtaa cacgtatgga agttccttgc tgtggtggta
     3121 tagtaaatgc tgttaagaca gctctacaaa atagtggaaa aatgatacca tggcaaatag
     3181 ttactatatc tgtagatgga aaaattgtag atggagaaat ttaattaagt gaattataga
     3241 aaaatataat aaattttaat aaataaaaag ggaggaataa aaaatggaaa aaatgttttg
     3301 ttttcaatgt gaacaaacag caggaggtaa aggttgtact ataaatggtg tctgtggtaa
     3361 aaaggggggt acagctaact tacaagacga acttacagga gtgttgattg gtctttcaag
     3421 agcaactatt ggaaataaga atagaccaac aagtgaaaca gataaaatta tgatagaggg
     3481 gctattcact actataacga atgtaagttt tgatgatgaa gctataaaaa gacaaattga
     3541 aaaaactgag gtagaaaaaa gtaagcttgt acctagatgt tcagattgtt caacaacttg
     3601 taatagaaat gataactatg atatgaagga tttatggaat gataatgagg acatacgttc
     3661 attaaaatca ttaatcttat ttggattaag aggtatggct gcctatgcat atcatgcaat
     3721 ggtacttggt tatacagaca aagacgtaaa tgaatttttc tatgaaggat taatagcagt
     3781 tggaggagac ttgtctattg acgaactttt aggtctagtt atgaaaactg gagaaataaa
     3841 cttaaaatgt atggaattgc ttgatagagc aaatactgaa acttatggaa caccagaacc
     3901 gactcaggta tctatgaaaa tcgagaaagg gccatttatt gtagttacag gacatgacct
     3961 ttatgattta cacaaattat tagaacaaac gaaagataag ggtataaata tatatactca
     4021 tggtgagtta ttaccagcac atgcatatcc aaaactaaaa gaatataaac atctaaaagg
     4081 taattttggt acagcatggc aaaaccagca aaaagaattt gataatattc ctgctccaat
     4141 attatttaca acaaactgtt taatgcctgt taaagatagt tatagagata gagtatttac
     4201 tacagaagta gttgcttatc caggtatggt tcatattgat gaagataaag atttttctag
     4261 tgtaatagaa aaagctcttg aacttggagg atatagagaa gatgttcata tgactggtat
     4321 taatggagga gatacattaa ctactggttt tgcaagaggt acagtttcat ctgtacaaga
     4381 taaagtagta gatgctgtaa agagtggagc tataagacac tttttattgg ttggaggttg
     4441 tgatggtgca aaacctggaa gaaattatta cactgaattt gtaaaacaag ctcctaaaga
     4501 cactgttata ttgacacttg catgtggaaa atatagattt aatgatttag accttgggga
     4561 tatatgtggc ttacctcgtt taatggatat gggacaatgt aatgatgctt atagtgcaat
     4621 taaagtggca gtttcattgg caaatgcttt tgaatgtgga gtaaatgatt tgcctcttag
     4681 catggtactg tcatggtatg agcagaaagc tgtttgtata cttcttacac tacttcattt
     4741 aggtatcaaa aatatctata tagggccttc tttgccagca ttcttatcta agaatgtatt
     4801 agatatattg gttgataaat ttgcattgac acctatatct actcctgaag aagatatgaa
     4861 gaagatttta ggataaataa acatatctat acttaaagat atttatagtt aaaatatttt
     4921 taagtatgcc acaattaaga taaggggtta aacttaaaat ttgattaaaa aatcaatttt
     4981 gagatgaccc tttatttatt ttttgtaata attgaaaaat aaacctgctt tcagtatatt
     5041 ctttttaaga atctttgtat taaatttata atacttagat atataatatc ttatatattt
     5101 tattttaagt attaaaggtt tgaatgtaac ttaaatatta aaagaggaac attgtaatat
     5161 gtaacttgca taaaatatat atactatagt gaaattattt tgattaaaat ttaaaatata
     5221 aggtgtttta tgtgggggta tatgtatatg aaaaattcta attttaatat attggcattt
     5281 gatggtggtg gattaaaagg ggcattgagt atatccatac ttgagaggat agtaaaagag
     5341 tatccaaatc tattaaacaa tataaatatg tttggaggta catctacagg gagtctaatt
     5401 gcactagggc ttgcgtatgg tgtaagccct aaagagataa aagaacttta ctcaattgaa
     5461 aattcaaagt atatttttaa taaaagttat gcagaaatac tcagaccaaa gtatgaaaac
     5521 aaaaacttaa aagaagtttt attaagtata tttccagagg aactagaatt aaaagattta
     5581 aataaattag taatgatacc aagtttttat ataggaaatg aggaaaatgc atggaaacca
     5641 gtgttttata acaatatgcc aaattctttt acaaagacta gcaaagttgt agatgtagca
     5701 atggcaagta gtgctgctcc ggtatttttt ccaacatata atcgtcatgt tgatggagga
     5761 ataatagcaa ctgacccgag tttagcgtgt ataattcatg ccattgacag tggatttaaa
     5821 cttaagaata caagactttt ttcaataggg actggatatg tatataacag tataaaggca
     5881 gatactactg aatggggagc tattgactgg ataattaata aagaacccga tttaccaatt
     5941 atatctatta ccttagaagg aaattctcaa atgagtcagt tattttctca aaaacttctt
     6001 ggagataatt actatagatt aaatcctaag atggaaaaag atgtagctat ggatgattgt
     6061 gatgcattag aatatctaat gtctttagga gaaaattgtg atataaaaga tgcttttgga
     6121 tggataaaga aaaagagctt ttatagaaat tagattcaat ttgcaattgt atttttacac
     6181 atgatttcat tgagtaggta aaaataataa aatgcagatt attaagttac ttcttaggga
     6241 ataaataaag tataatttat ttaaggagtg atgaaaatga taaaacttgc tacttttgca
     6301 ggtggatgtt tttggtgtat ggtaaaacct tttgataagt atgatggagt tattaaagta
     6361 gtttctggat atacaggtgg tcatgttgaa aaccctacat atcaagaggt atgctcagga
     6421 actactggtc atttagaagc aattcaaata acatatgatg atgatataat aagttatgat
     6481 gaacttctaa atatcttctg gaaacagatt aatcctacag atgatggtgg acaatttggt
     6541 gataggggaa ctcaatataa gactgctata ttttatcatg atgaagaaca aaaaaatata
     6601 gctttagagt caagagaaca tctggaaaat agtaaaatat ttgatagtaa aatagtgact
     6661 gatataattc cagcaactaa attctacgaa gcagaagaaa atcatcagga ttattataag
     6721 aaaagaccat taaactacaa tatgtattat aaagggtctg gaagatataa ttttgtaaaa
     6781 agccattggg atggaaacaa tataaatagg gaagagttaa aaaagacttt aactcatata
     6841 caatttgaag tcactcaaaa tgatatgaca gaagtacctt ttgaaaatga atattatgac
     6901 aataacaaag agggaattta tgttgatatt gtaagtggag aagtgttatt ttcttcaaaa
     6961 gataaatttg attctggatg tgggtggcca agtttttcaa agccagttat agataaatct
     7021 atcattgaga gaagtgattt ctcacaaggt atgtacagag ttgaagtaag aagtgctaaa
     7081 ggaaattctc atttaggtca tgtatttgat gatggtccaa aagaacttgg aggtttaaga
     7141 tattgtataa attctgcttc tcttaaattt ataccaagag aaaatatgga agctttagga
     7201 tatggagaat atttaaaact ttttgagtaa ttaggaatat ttattataaa cttatctaaa
     7261 aggtgaaaat ataaaaaaat attttcacct tttgtttttt tgtcaataat atgataaatt
     7321 attaattgta atcttattta taccaataga ttaagaattt aattttgaaa gagagaaaac
     7381 aaatgaattt aaaagacttt aatggaaaga gattaaaatc agctagaatt tttagagcaa
     7441 agacgataga acagttgtct aaagaaacta aaataaataa aaaagattta aaagcttttg
     7501 aggaaaataa gtatgtacca aatatagaaa atacattaaa attatctaat atattaaatt
     7561 ttccaaaaga gtatttttat aaaaatgaaa atataaatat cgttgtagag gatagccact
     7621 ttaaccctca atctaaacta cctagagtag aagagatttc ttatagagaa aaaataatta
     7681 taatacataa aatatattca tttatggaga attatattaa gtttccaaaa gaaaacttgc
     7741 caaatagaaa agtaatgtca gatattaata taaatgatat agaatctctt gcatataaga
     7801 ctagagagtt ttgggattta aatagtactc caatagtaaa tatggtaagt ttgttagagt
     7861 ctaaagggat tataatatca ggtatgaatg tagatagaaa aggggcaact atctttactc
     7921 aaaaacagag aattagtaaa gaatctaaat atctaatatc tctgggtaat gacaaaaagt
     7981 cagcatcaat tagaaattat actttggcat gtgagcttgg atatataata tcaagtgaat
     8041 taagaatacc aagtaagcaa ttttcagaag atgagtatgc ctgtgcattt ttattgccaa
     8101 aagagagttt tttaaaagat ttgactcatc cagaagattt agattattat gtagaactaa
     8161 agaaaaaatg gattgtacct atatctgcta tgatttttag agcatataat ctagaaaaga
     8221 taaattacaa aaaatacaat tatcttatga atgaaatgga taaaaaaggc tggttgatag
     8281 aagaaccact agacaaaatg aaaggttcaa gcccaacgta tttaaaaaga gcagttgaat
     8341 tattgataga aaataaaata atgagtgtaa atagtatagt atcaagttta gaggaatttg
     8401 gaataaatct ttatccagaa gacttagaat tattgatggg attgaaaaaa ggattgttat
     8461 ctcaagaggt aaataaaaaa agtaaagtta taaaatttga tggtaaaaaa taggtttata
     8521 ttttagtatt aaaattaaaa gcaggtacaa atgatttaaa cattaactta gttaaaagtc
     8581 ttttcaataa gtcatttacc tgcttttagt gtaagaaaga atattgaatc taaagtgttt
     8641 ttgaaagtgc aatatattcc cttaaaacag aacaagattt attaaattcg ttttgctcat
     8701 attcagtcat attgatttca ataatttctt caataccatc ttttcctata acacatggaa
     8761 cacctgcaaa tacatttctt tcgttatatt gaccttctag taatgtagaa actggaagaa
     8821 cttttttctc attgtgtagt atagctttta caatttctac taaagcagta cctataccaa
     8881 attctgtaca atctttacca tttattatac ttattcctgt ttttttattt ttttcaacta
     8941 ttgatggtaa gtctaaattg ctataagtac ttgggttgtc ttttatcatg tctaagattg
     9001 gtttaccacc aattgaaaca tgagaccaag gaaccatttg agaatctcca tgttcaccca
     9061 tagaataagc ttgaattgat tgttgagcga ttccagtttc ttcagataaa atccttctaa
     9121 gtcttgttga atctaaagct gttccagttc caatgacttt gtttttagga aagccagatt
     9181 tttcccaaat ataatttgta actacatcaa caggattaga tatgactaca aatataccat
     9241 caaagccaga atcaactatg ggttttacta tagattttat cattccaata gttggtctta
     9301 gtaaatctaa tctagttgtg ttttgtctac gtaaaggccc ttctgaagag tctgcaacac
     9361 ttatcactac tatgtcagaa tctttacagt cagaaaaagt tcctttttct atatgtactt
     9421 tatgtggcag atatgaaact gcatcagcta aatctaaagc ttgagctttt gatttagatt
     9481 catctatatc aatcataaag agttcatcac aaactccttg agttattaaa ctaaatccac
     9541 aatgagaacc aacatgccct gaacctacaa tagatatttt tcttggcttt attgccattt
     9601 taaatcactc cccctaaaat aacgatacat tgaaaattat agatgttatt aaaagctgtt
     9661 tttggatatt ttaatattct attatttcag aaaatttatt taatatgatt ttatactaaa
     9721 atttttaaaa aagcaatgtt aatatactct ctattagtga atattaaagt attatatgta
     9781 tattaataat tattaaaata tgtaatgtca ctgatagtta gaatatttaa gtgctaaaag
     9841 atgtgtataa ataataatga tgtcagttta taatgatatt aaattgagat atactgtttc
     9901 tctaaattaa aaaataaagg agtaaatgtg ttatgaatta tcatacaaaa gaagaattgg
     9961 tggaagcact gagagtagta tcttcaagta ttattaattg tggaaaagga cagaagaaat
    10021 tttctgagga gacatctcat catacacgtt ttaagaatat tatagaagta atgtatattt
    10081 caaaatcatt aataatggat gaaattagta aaagagattg aagcttattt tgcaatttaa
    10141 catgttttaa tatacatttt attatataag aaaataagta aacatacatg ttataaaata
    10201 ttggaataat atacaattat aaatttgtta acactaatat ctatatgtta aaatatagta
    10261 gaagatgtta gtaaataata ggatgagttc tattttaata caattaaatt aagcttagtt
    10321 tgaaatttgt atctaaaaag taaagtaatc tagaattaag aaaatggaga tagaattata
    10381 atggaaaagt tatattatac aaatcagtat ataagagatt ttacagcaga aataattgaa
    10441 ataaaagaag tagaaaataa gtatcatgtt ttattagata agacagcttt ttttcctggt
    10501 ggaggaggtc agtttggaga cctgggaaaa atagagagtg atgatgtaat aaatgtgtat
    10561 gaagatggaa aaaaaatata ccatgtatta gataaaaaac ctataaagat acataaggta
    10621 aagtgtagta ttgattggga tagaagagaa gatggaatgc atcaacattt tggacaacat
    10681 gtattgtctg gatgtttttt taaaactttt aatgcaaata cgacaggttt tcatttggga
    10741 aatgagtata gtacagtaga tatagaaggt aatttagaag aggataaaat tagagagata
    10801 gagttgtttg ccaatgaaat tattcgtcaa aatatacaag tagagacatt acttccaacg
    10861 aaaaaagaac taaaaaagat ttggttaaga cgtgatttgc caaatactga tgaagagata
    10921 agagtaataa aaataggtga tttagatacg aatgcatgtt gtggagtgca tccaaaatca
    10981 actggtgaat tgagaatgat aaaaataaag aaatgggaaa aacataagtc aagtacaaga
    11041 atagagtttc tagcaggtaa aagggcagtg gatgaagtat tgaagaggga tatatatttg
    11101 actaaaatat gtagatattt aagctgtagt gaggaagatg ctataaatgg tattgtaaat
    11161 ctaaatagta aagtggaaga ggctttaatt aaaaaaagaa ggcttgaaga agtagtatct
    11221 acatatcagg tcaaagaaat gattgaaaaa gcaaataagg ttggcaatat ttcggtcatt
    11281 aaaaaaattt atgatgatga agatttgaaa tatgtaaata agatagcaaa taaaattgca
    11341 gaatatgaaa atagtattgc attattagct gttaaattta atgataaaat aaatttagtt
    11401 tttgcatgtt ctaaaaattt aaaagatatt aatatgggag ttttattaaa ggatgcaata
    11461 agtcttatag atggcaaggg tggtggaagt aaagtattag ctcaaggagg aggtaagaat
    11521 aatggaaatt tagaggctac acttgattat gcatttatga agatagaaaa aactctataa
    11581 atatattata taaaaaacct tagtagataa aatagatatg gaggaataag tatggagcaa
    11641 caaaaggact acatagagtt atatgaaaat gaagaactaa tagagataag agaagcacgt
    11701 atggatgatt tggatacaat agcaaaattt aattataatt tggctaaaga aactgaagga
    11761 aaagaacttg atatggatgt tttaactaaa ggtgtaaaag cattgttatt agatgagaga
    11821 aaaggaaaat atcatgtata tacagtattt gataaagttg ttgcacagat tatgtataca
    11881 tatgagtgga gtgattggag aaatggaaat ttcctttgga ttcagagtgt atatgtagat
    11941 aaggaatata gaagaaaagg tatctttaat tatttattta attatataaa aaatatatgt
    12001 gataaagatg aaaatatagt tggaatgaga ttatatgtag aaaaagaaaa cataaatgca
    12061 aaagcaacat atgagtcttt aaatatgtat gagtgtgatt ataatatgta tgaatacgaa
    12121 gtaatacatt cgtaatataa atttgtaata ctgttatact gatgttataa agtattgatt
    12181 atattgacga ttatatgaaa taaactggat attataatta gaatttttag ggggtataaa
    12241 aatgggcgtt ttaaaaggaa tgttttatgt atttttattt ttagacttag tgtcaatatt
    12301 ctgtttcttt ttcaataaag gaaaaatagc atctaataaa attgtgttta atgcaatagg
    12361 agtacttaca tttgtacttt gttttatgct ttttagttat tatcctaata ataatcttat
    12421 tggaaaattt attgcttctc tattttttat atttggagtt gcaggagtta tgttgaaaga
    12481 aaaaaatttt ttatatgcta gacttctttt aactgtagtt atagtttttt ctacattaag
    12541 attgttcgtt attcaataaa atgtatttgt atgaaaattt attaaagtct acacatttaa
    12601 gtgtaggctt ttttgatttt ttgtttgaaa attaaggaaa ttttaaggaa attttaaaat
    12661 cgtcttaaat atttattgat atacttaaaa tattaatagc gaaaggagac ttatcagtga
    12721 tagaaagtaa aaagtcagaa aaattgttac tgtcaattat atacataata tttttggtat
    12781 atattttaat gattataaga ataattttat ttagagatgt accaatttac gctattttca
    12841 agggaacgtt taggaatgta aatttaattc cattttatac aatatatcaa tttatagtag
    12901 atagtaatct tgactttatg aaagctactg taaatattat agaaaatata ggtatattta
    12961 ttcctatggg aattttttta cctatagtat gtaaaaatct aaataaaaaa actataatta
    13021 tagtaattat acttgttagc ctagcatttg aattaacaca atatattttt gctcttggaa
    13081 gtagtgatat agatgatgtt attttaaatt cattaggtgg cataattgga attaatattt
    13141 acataaacat gaataaatta tttcccaatg atattaacag atttaaggct ataataggta
    13201 ctagtttagt tttaggagtt ataggattag gagttatttc taaaaattat cataacttac
    13261 taacttttaa atttagacct gatagaaaaa tatcaaaaat attaattgaa gaaaataggg
    13321 aaataattaa agatataaat aaagatacag tagatatagt tgggacattt gagagcttta
    13381 aaaaaggaat aataactatt aaagcaggtt ctaataacaa agtaaagaga ccagagaatt
    13441 taattgattc tgatggaaat ataagaattt atttaaatga aaatacaaaa ttagtatctt
    13501 atataattac caaagaatca aaaatggata tagttaaata tgaggaattt gatgtgaaga
    13561 atttagattt attaaggaga tatgatacta ttaatgtttg gattgataaa gaaaatcagc
    13621 caaaggataa acatggtatt atggctagta aattattaat tggattgtat gaataaaata
    13681 ttaaacaaga ataaaagaat aacaaatata aaaaacgaaa aaactatttc tataattatt
    13741 tttacgttgt gcataaaata agaacattag tgaaggagta gagataaatt atggagataa
    13801 taaaatatat cttgtacttt tttatatatt catttttagg atggacagta gaaagtatag
    13861 gatgttctat agcaagcaaa agaataatca acagaggttt tttaaatggt ccaatatgcc
    13921 ctgtttatgg ttttggagcg gtaattgtga tatcactatt gggaagattt aataatgtag
    13981 taatagtatt tctattagga atgattttaa ctacgatact agaatatttt acaggattta
    14041 ttttagaaac cttatttcat gctaaatggt gggattattc tgacaaaaaa ttcaatataa
    14101 agggtagagt atgtttaaaa aatgcaatat actttggtgt aatgtctgtt ttaataataa
    14161 gatttataca tccatttata aaatattttg taagtataat tccatataga atattaattt
    14221 caatggctat aattacaaca ctttggacta tacttgattt gattgttact attataacac
    14281 ttaaaaaatt agatataaag ttaaacttat tagacgatat aattacagac cttaatgata
    14341 taaatgtgaa attggataaa tttgatagag gagaaataca agctttattt aaaacaataa
    14401 ataacgacga attagaagtt agagaaaaaa taaacaaaat aaatagtaag ttggaccgta
    14461 tagagtctaa tattatttta caaaaaagag ttataaaagc ttttccacat ataaaacata
    14521 aaaaacaaca agagcaacta gaacatttta aaaaaattat taatgagaaa aggtaattat
    14581 acaatttaat gaagacgaat agaataatgg tatttataag atgattttca gaggactcta
    14641 taaaaataga atagagtcct ttattttttg aaaaataatg tttatttgat aaaaataaaa
    14701 tatattgttt ctgaataaca aatattttga aaattataaa aacatatatt gacaatttgt
    14761 gtcaaaaata ctaacattaa gtatataaag aattttatat gagaatatct ttataaatcg
    14821 caataatttt aaaacataaa tttatttaaa gaggggatga tttgccaatg agcgagataa
    14881 ctagtcaaat gataagtaca gaagaaaaaa aatatgttgc taaaactcaa aagatacctt
    14941 attatccagt agcatttaaa tcaggtgatg gtgcaatgct atatgattat gaaggtaatg
    15001 aatatgtaga ttttttagca agtgcaggct ctgcaaatgt aggtcatggt aataaggaaa
    15061 tctcacaagc agttaaagaa caaatggatg atataacaca atatacactt gcatatttcc
    15121 atagtgatcc tcctgtaaaa ttagctgaga agcttgtgga aatagcccct ggagataatg
    15181 ataaaaaagt actatacagt gcaactggtt ctgcttgtat agatgctgct ataaaattag
    15241 caagagggta tactggaaga acaaagataa tatctatgtg tgaatcttat catggaagta
    15301 cttatggagc tatatctata tctgctttaa gtacaaatat gagaagaaaa atgggtccat
    15361 tgttaccaga agtttatcac ttccattatc ctgataaaaa tagaacagct aaagaatgtt
    15421 tagatgagat agaatatgct tttgcacact atctaccagc agaagaggta gctgcaatat
    15481 ttatagagcc aattgcagga gatgcaggaa taatagtgcc tccagtagag tgggtacaag
    15541 gtttaagtaa aatatgtaaa gaaaatggaa tattacttgt aagtgatgaa atacaacaag
    15601 gtatgggaag aacaggtaag tggtttggta tagaaaattt tggtgttgaa gcagacctta
    15661 tagttcttgg aaaatctgtt ggaggtggat tacctctagg agcagttgtt ggaagaacag
    15721 aaataatgca aagtcttgat gcaccagcac atttatttac tttagctgga aacacaacag
    15781 tatgtgtggc tgctttaaaa tctatagaga taattgaaaa agaaaactta cttcaaaaga
    15841 gtatagaaat gggagattat attaaagcag gatttgaaaa actaaaagaa aaatacgata
    15901 taattggaga aataagaggc ataggattat ctataggagt tgatatagtt aaaggaaaag
    15961 gttctaatga aaaacatcca gatgctacag ctaagatatg ttatagatgt atacaaactg
    16021 gattaataat gatattctta ggacaatcta ctttaagagt gcaaccacct ctagtaataa
    16081 ctaaggagca agtagataaa gcaatgaata tcatagattc tgcaatagat gattatttaa
    16141 atggtagaat tggtgatgaa gtatatgaag taactcaagg ttggtaaaat aaatattaaa
    16201 tgaaaatagg agttatctca attttagaaa attatttctg aaaagagata actcttttaa
    16261 gttacccaca aataagaaaa ttgtttgatt cattaaagac tatatttcgg atgtcgtttt
    16321 tgtgagtatc agagacagta ttacgagaat aattaccttt attcgtacta tctgttaatt
    16381 tagttataat taagatagct aaaagaataa gaattactgt gtataaagca ataacatatg
    16441 gttttctttt atatttgtat ttgttattga aaaaagaatg gccataatta tttttattta
    16501 ctttagatgt atttgtattc tttttcttgt tacctttctt tttatcatcc ttttttctat
    16561 ttttagggta aggaatgact ttgtgtgtat tatcaagttt gatattttta tttttttgaa
    16621 tattgttttc caggctagct tttttttctc caaattgaat agttttattg gtattgttgt
    16681 tatctttcaa tgttaatctc cctcccttaa actttccctt tagataatat tactactaac
    16741 ctatggaata tacaataaat aatatataag taataaaatt ttataggtat aaatctcttt
    16801 ctccacgctc taaggcagtc atattgttag atactatgtt tcttatatct tctctttcta
    16861 tcttacctat ttcttctgcg ataagttttt tacccataat tttaagttct tcatctccat
    16921 aatcgattaa aaattcattt aatgtagtaa gagcatttgg tgtacataca ttatggattt
    16981 gtcgactttt agctaagctc atgaatctat cgccagttct acctttacga taacaagctg
    17041 tacaataact aggtatatat ccatcagtaa tcagttcttt aagcacctca acagggcttc
    17101 tatggtctcc aacttcgaat tgttctgtat tgtcaccatc ttcatatgct ttatatcctc
    17161 caacacctgt tagagaacct gcactaattt gtgatacacc atattttagt aactcatttc
    17221 tcatttcagc agtttctcta gtggacatta tgattccagt aaaaggaaca gctaatctag
    17281 ttatagctac tatttttttg aacatatcgt cagaaactaa gtgaggaaaa tcttctaagc
    17341 tcataccttc agcttttttc aatctaggga aagatatagt atggaaacct acaccaaatt
    17401 tttcctctaa gtgttcgttg tgcatcataa gtcctagaac ttcaaattta gggtcagata
    17461 aaccaaataa aactcctgca ccaacatcat caacaccagc ttccatagct ctatcaaaag
    17521 ctgttaaatg gtaataatag tcatttttta tagattgacc atgcattttt ataaatgttg
    17581 gtttgtgata agtttcttgg aataatatat aagttcctat tcctttttct tttagtaatt
    17641 tatactcatc tacacttgta gctgctatgt taacgtttac ccttcttata tttccgtttt
    17701 cattatatgt tgaataaatt gcatctaagc aatctaagat gtaatttata tcacaatttt
    17761 tagggtcttc accagcttct agagcaagtc ttttatgacc cattttttct aatattttaa
    17821 cctcttccct tatctcttcc atggtaagtt tttttctttt aaatttgttg catttactaa
    17881 aaccgcaata aacacattca tttacacagt agtttgatac ataaagaggg gcaaataaaa
    17941 ccactctatt accatatatt tcatttttta tttgcccagc tatactaaat aatctatcta
    18001 gttgtttttt atcttctact tcaagaagtg tagctatatc tttataacta agtttttccc
    18061 ttctatcagc cttatcaagt accttttcaa tatcatcaga agttgaattt tttgcatctt
    18121 ctaataaaga attaataagt tcatggttta tgaacattta caaacgcctc cattcaatat
    18181 tatcaccgcg agaatagtca acttcaagtc ctaatgactt gatgttttct tctaaacttt
    18241 ggtgatattc acaagcttct tgaccagtaa aggctttgtt atcgtaaaga gaatattgtt
    18301 ttctaaattc ttgaggagaa aggtttggca taattacatt gcaaccagct aaaagacctg
    18361 catttctacc actagggttt atagatgcca aggctgttgt agcgggtaat aaaacttttg
    18421 gaagtagaag tcttgtaatt gaaagcataa gtaatgtctt ctctaaatct ccatgtttat
    18481 aatccttaaa aattgtatca tggtgtggaa tgaaaggtcc tattccaacc atatgaggat
    18541 ttaactcttt taggtataat aaatcacgaa caaggtcttc gttgttctga aatggtgatt
    18601 caaccataaa tccagcacct acctgataac caatttcttt aaggtttttt aggcattctt
    18661 ttctagttct aagctcaatg tttggcggat gtaatttttt ataatgactg tctgtagcag
    18721 tttcatgtct taaaagatat ctatctgcac cacataaaaa gtatttttta tacgaatcat
    18781 aactcttttc tcctagtgat agagttatgg cacaatcctc atatttattt tttatacttg
    18841 aaacaatttc acatatttta tcatcagtaa aatatgcatc ttcaccacct tgcaaaacga
    18901 atgttctgta tcctatttta taacctatat cacaacattc aagtatttca tcaagtgaaa
    18961 gcctgtatct tatgatattt ttattagaag attgtattcc acaatagtag caattatttt
    19021 tacaatagtt tgtaagctct ataagtcctc tcaagtagac tctatttcca taatgtttat
    19081 ttctcatgtc gctagcttgt ttataaagat agtttcttaa attttgttcg aagttatatt
    19141 cctcattgtc gatattttct attaaatata gtagttcatc aaaacttaat gaattaatgc
    19201 tgtaaagttt atctataaag tatttaatct tatttttagt catattcaaa tatataatta
    19261 ttaagtatag ttaaattttt aataattgaa aagttcaagt gatctttcca aaataccttt
    19321 tacatatgct attaagatac catagtttac aataggtaca ttgaaagatt ttgccttttc
    19381 tattctagat aacattccag ctctattcat catacaagct ccacagtgaa ctacaagggc
    19441 atatttattt atatcttcag tgaaggatac tccagaacta aattcaaaag taatgttttt
    19501 gccagttttc tttctaatca tattaggaat ttttactgtt ccaatatcat cagtttgtct
    19561 atgatgggta catccttcgg ccatcaagat tttatcacca tcttttaaat tttctaaagc
    19621 ataagcgcca ttaatcaatt ctttaaggtc tcccttttgt ctagcaaaca atatagaaaa
    19681 agatgtaaga ggaatatctt ttggtgtatc tttatctact tgtggaaata cttgagagtc
    19741 agtaataaca agttttggtt ttttgcctaa attagataga gtttctttta aactatcttc
    19801 ttttgtaact atagaaattg caccactatc caaaatatct cttataactt gctgttgagg
    19861 aagtataagt ctaccttttg gagctgcttt atctattgga acaactaaaa caattaaatc
    19921 atttggttca attaaatctg ataccaattt gaactcagtg ctatccttag gcaaaacttt
    19981 tattatttca ttttttaaat tttcaattcc aattttatct gttgatgaga cagaaacaac
    20041 tggacattta actttatttt tagttaagtt taaaatttct gattgattct taatagtatc
    20101 aattttgttg agaattagaa tatgagggat attcttatca ttaaactttt caattagtga
    20161 caaatcttct tgtgatatac caatctggca atccactact agtaaagcta tgtctgtctt
    20221 ttctaaaaca tctagtgatt tacctattct aagttcacca agttcaccaa catcatcaag
    20281 accagcagta tctataagaa cacaaggtcc tataggaagg atttccattg gtctaaatac
    20341 tgggtctgta gtagttccag caatatctga gactattgca gcactttgat ttgttatagc
    20401 atttattatg ctagatttac ctgcatttct tttaccaaag agacctatat gtactcttac
    20461 cgattgtgga gtagaattta gactcatttg tactcaccac cttaaacttc tttttttgat
    20521 atactagttt ttacagatac atgaggtatg ttgcccaatt taccagtcaa actattaata
    20581 gtatccattt cgccaacaac tgctatacat acgattgaga caccttcttc ttcgcaagga
    20641 atgcccattc ttcctttaat aataccttta aaacctgata caacctcatt gaatttaaat
    20701 tgacattctt ttggctcttc caaaatagca cttataactg caaccttttt cataaatatc
    20761 ctcctaactc agaaaatatg tcttaaacaa atccaaaatc aaaaaacttt tctttgtaaa
    20821 gaaaagtttc tacaaaagaa aagttattaa ttacacaagt ataatgtgct atattaaact
    20881 atttccttac agattagata actcttttct ggtagggtta gcttgtaaaa atttaattta
    20941 acaacaagta actgaagaaa atttttatac aatttatata aattataata aaaattaa
//
LOCUS       DAMJWU010000060        20743 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMJWU010000060 DAMJWU010000000
VERSION     DAMJWU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.060"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(85..621)
                     /locus_tag="PC460_002399"
     CDS             complement(85..621)
                     /locus_tag="PC460_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2601280.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(639..1952)
                     /locus_tag="PC460_002400"
     CDS             complement(639..1952)
                     /locus_tag="PC460_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2601281.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2307..2747
                     /locus_tag="PC460_002401"
     CDS             2307..2747
                     /locus_tag="PC460_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2601282.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2932..3120)
                     /locus_tag="PC460_002402"
     CDS             complement(2932..3120)
                     /locus_tag="PC460_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601283.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            4060..4455
                     /locus_tag="PC460_002403"
     CDS             4060..4455
                     /locus_tag="PC460_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601284.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4498..6597
                     /locus_tag="PC460_002404"
     CDS             4498..6597
                     /locus_tag="PC460_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF2601285.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYNGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6822..12032)
                     /locus_tag="PC460_002405"
     CDS             complement(6822..12032)
                     /locus_tag="PC460_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2601286.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKEIM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNALYEEHKDWIISKREDSGSA
                     GAEGTAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILKSLGYIDFKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12047..14542)
                     /locus_tag="PC460_002406"
     CDS             complement(12047..14542)
                     /locus_tag="PC460_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2601287.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15225..15512)
                     /locus_tag="PC460_002407"
     CDS             complement(15225..15512)
                     /locus_tag="PC460_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601288.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15532..15807)
                     /locus_tag="PC460_002408"
     CDS             complement(15532..15807)
                     /locus_tag="PC460_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601289.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16444..16623)
                     /locus_tag="PC460_002409"
     CDS             complement(16444..16623)
                     /locus_tag="PC460_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601290.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(16908..18383)
                     /locus_tag="PC460_002410"
     CDS             complement(16908..18383)
                     /locus_tag="PC460_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2601291.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
     gene            19452..20630
                     /locus_tag="PC460_002411"
     CDS             19452..20630
                     /locus_tag="PC460_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2601292.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFRNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
BASE COUNT         7028 a         3532 c         2246 g         7937 t
ORIGIN      
        1 acatataata ttaaaactct ttatttttta atatatattt taaattttct tttttaataa
       61 atctttttct tttaataaat ctatttaatt ttcttcttct atatcagagc caaagggaat
      121 ccatgcatta ccaaactctt tatctttaaa taaataagct aatccagtta tttgcttcaa
      181 tctaagaatt tcatcaactt ccatacccag gtgctttgat atccattcat cagattttcc
      241 aatttcacaa agttcagtta taattttact cattaaatcc acattatgag aacctcttgc
      301 acgattgtgt cgaatagtac ttgccattct ttcatcaaga gatttatcaa ttactgaaac
      361 aggaagaagt ccattttctc tatcatatat atcctcattt tcaagcatta ctctatatct
      421 atgaaaacca tctacaataa tatacttatc cttttcctca ttataataac agacaattgg
      481 cattgtatac ccatcttctt ttatactttt atacaacaac tgcatttctg gtggggctac
      541 tgcatttgga ttatagtcgt tagattctat cttttcgatt ggcactgcga tgattccata
      601 tacaggactt ttatattcca tagcatctta actcctatct aatgaatttt ttcatatttc
      661 ttcttaatat attccgttct aattctctgt tcttttgtag gaccaaatcc cataaatcta
      721 cacatatggt cattttttaa aatacaataa cacattcttt tccaacttgg tatatctttg
      781 gtaaatttaa tatcatccgt atcatcaggt atatcatcca aaaatatgac tctactcttt
      841 ttatcttttg tatgatttga aactccattt aattctattt tataaccttt tctcaacaat
      901 tcacgtataa ctatttctga caaaccacca cctgtattct gccaaaactc aatagatgtt
      961 gaaaattttt taatatagct attccttata ttttcaggta aggtatcaag caaaaaatat
     1021 gtgaaacttt tccatgtgtg tccatatggc aatttaacac tacgatatcc catagctttt
     1081 gttttgccat aaatagaagt aaaattaact ccttgtactc tagcaaccaa tttcacccat
     1141 acatctgggt ccaatattct gtataaattt aaactctctt ttgctgaatc attaaaaggt
     1201 gatgccactc tcatttttct tggagctact ccagctttat agaataagtc ataaatttta
     1261 ttatagtcat agtcaaattt atagtgtgct aaccatatat cttctatttc ccaatcatat
     1321 ataggagatg ccgtccagac atttttaaat tccttagtta tccagcactt tcctttatat
     1381 ccatgctgtt tattcacaat agaacaataa cgttgtagag attctgctgc tcgtgttcct
     1441 aataagcaaa caacttttgc accttcatgt atatctctaa tccatctacc aaattgtttt
     1501 gccaaacttt cttgtctcat tttatattta taataagtaa aaggattatt tttttgattt
     1561 attatgtatt ctttgactgg catttccctt acccataact cttctttctc atcatcccat
     1621 ggataccaat acatttcata atttcctaca gcagttcttg tagccattgg taaacacacc
     1681 caatatggtt caatatcttc aatattatct tcaaatactt tttcaacata ttcagtagtc
     1741 aaactatatt gagcctcaaa atcttgatga aatactccta ttttggaact tatattattc
     1801 tttctcttat aatccaaaac taaatttagc agtaaaccac tatcttttcc tccgctaaaa
     1861 gatacataga tataatcaaa ttcattaaaa agataagaaa gtctttcttg aaaagcttca
     1921 aatacatcgg attctaaata tctcttatcc atttgaatta tcccctttca attagaagtt
     1981 tataaaacct tacaatgcaa tattattatg cttttttagc agtaatcagc ataaattata
     2041 cattatttaa atacacaaat ccaactaact acatataatt ataatgtaat tatacttttg
     2101 tattataaac tatattttat cattatttta catttttttc caatatatat aaatatcata
     2161 tttttctatt agctaaaact aattacttta tatttctacg tattagtata ataatacata
     2221 aaaacttgaa taatgcataa aaacttgaca tactgttgtc ataattactt tgctataatt
     2281 aaaagtaaaa atggaggtaa attctaatgt atgaacgtat gcttgataaa agcaataaac
     2341 cctgtttgtc tcaaataata gaatatatag gtaaagatgg atatgcacga ttgtgtaagt
     2401 tggaaactta cttgaaaact caatataatt tatcaagaga acttcgattt ccttttggaa
     2461 acaattatgg ttggggttac aaatatagcc ataaatcttt tcatttattt tatgcttttt
     2521 ttgaaaaagg tgcatttact gttctgattc agataggtga caagcaagtt ccagcagtag
     2581 aaaaaacgtt ggtagctttg acaccaaaag ctaaagagct ctgggtcact cgttatcctt
     2641 gtggcaataa tggaggctgg attaagtatc gtattttaga agatgcagac ttacttgata
     2701 ttattcaata tataagagca aaaaaatctc cacctaaaca aaattaactt ttattttttg
     2761 tgatttaaat atagtgtata taaattccat tctttccata taaaaaaaat aatataaaaa
     2821 atatctatta gtataactta tttagtatgt cttattctac tttttacaga taattaaaaa
     2881 gttttatcat aaataaatca taactagaca tcctagtaaa tcaaaattaa tttattcttt
     2941 tttattagaa tttttgataa cagtccctat acatatgcca attatcattc caaaaattag
     3001 accataacac attgtgatac ctacatttcc tttagaaata cctatagcac aacctatagc
     3061 cattccaagt atcatgccta taaacataca tataataact aaattgttgt ttttattcat
     3121 agttttctcc attcaatata ttttaaattg cctagctcgc cttcttagct taaaacaaag
     3181 aaatataaat taactttctt tcattctaat atctgcttgt ataaatgtca aacaacacat
     3241 ttcttgtcat aacctcctct ttttgaagta agtacaatct cccagacgca ataatatcaa
     3301 tttcctaaaa ccatttaagt aacttttata tttacataat atttacaatc ataaactata
     3361 tttttattca tttatacatc ataaacactc cctactaatc attattatac ttaaaataat
     3421 gttcatttat aataatatat ctaacacact ttaattgaaa aaaataaaac tttgcctttc
     3481 attcccatat ataaataagt atactttttg atgctctatt acaaatcaac taaattaatt
     3541 gatataaatt atcattttct gttgaaaata ccattcgcac atgctatact taattgcata
     3601 aatggtaata aatgtcatat gattttacaa atctattaat aacaattatt acttttactg
     3661 tattttaaat tttttggact aaatatcaag aactttctaa aatacaaagt acaaaaaatt
     3721 tatataagtt ataaatttat atgaactaga taagctcaaa caatatgtta tgtataaact
     3781 gtgtagtatt cggttataaa tttatataaa ttatataagc tagataaagt attacctcaa
     3841 ctatataagc tagatatata aattgcatat ataagctata taagaattat ttcaactata
     3901 taaactacat atgtaaaatt catgtaaatt tactaatata aatcaaataa aattactaaa
     3961 ctctctaaac taattttgta aaacatactc tatttttaac atttgttacc ttatacataa
     4021 gtacaaacaa aatctaacta aatgaaagga taatgtaaaa tgaatatttt aaaaatgaaa
     4081 aaaacaggga tatttttagg aggagtactt tttggtactg taggtgttaa gatactttca
     4141 agtgattgtg ttaaaaaaac ttgcactcgt gcaacagcag gagcattgaa agttaaagat
     4201 tctgttcttg aaacaactac gaaaattcaa gaaaatgtag aagatatttt agctgaagca
     4261 aaagaaatca acgagcaaga agacttatct acatctaagg taaacgttgt acaagatact
     4321 cttaaatgcg aacaagaagt tcttaaaact gaaactataa aaactgtaga agatactaat
     4381 tctaatagca ttaaatgtga agaaatcaaa gataacgtaa ttgaagaaaa aaatagtact
     4441 tgtacaacag aatagattta atgattatta tgattaataa tatcaggagg aagtaaattg
     4501 aatttttata taaaacacga aataagaggt cgtatacgct ttgacttaca acttggcaaa
     4561 ttaaacaaca agcagtccga tactctctta tattatttaa cttctataaa tggtattacc
     4621 aaagcaaaag tttacgatag aactggtgat gcagtcattt attacaatgg tgaaagagaa
     4681 tacataataa gagaaatcac taaattcagt tttaacaata gcaaaataga aaacctagtt
     4741 cctgaacata ccagcaggga attaaacaat tattatagag acaaattagc aaataaactg
     4801 ctagtcaaaa gtatctccaa aatatttttt ccaaagtcct taagattttt tttaattgga
     4861 cttcattcta taaaatattt taaaaaaggt ctctcttccc tgctaaataa aaaaatagaa
     4921 gtttctgttt tagatgccac tgctataggt atttctattt ttagaaaaga tatcaatacg
     4981 gctggttcag ttatgtttct tcttggtatc ggtgaactat tagaagaatg gactagaaaa
     5041 aaatctatag acgacttggc tcaaagtatg tcactaaaca ttgaaaaagt atggttaaga
     5101 aaaggtgaca ctgaaatcct aattccaatt tcagagataa aggaaggaga tttagtaagt
     5161 gtaactatgg gaaatatgat tccattggat ggagttatag tatcaggaga agcaatggtc
     5221 aatcaggcat cacttacagg tgaatccctt gcagttaaca aaaaagaagg atcttacata
     5281 tatgctggaa ctgtaataga gcaaggcaat attgttatgt gcgtcaagga aaaagctggt
     5341 actactagat tccagaaaat agtaactatg attgaggaat ctgaaaaact aaaatcttct
     5401 gtagagagta aatttgaaca tctagcagat acattagttc catacagctt tttaggaagt
     5461 atattaacct atgcaattac tagaaaccct ataaaatcac tctctatatt aatggtagat
     5521 ttctcttgtg cattaaaact ctctatccct atttcagtac tatcagccat gagagaatgt
     5581 aacaataaca acattactgt aaaaggtgga aaatttcttg aaggtgttgc atgtgcagat
     5641 actattgtat ttgataagac aggcacatta acaaaagctc aaccaactgt ttcagatata
     5701 attacattcc aaaattataa taaggaggat atgttaagac ttgcagcttg cttagaagaa
     5761 cacttcccac actccatagc aaatgcagta gtttatgaag ctgaaaaaca agggcttagt
     5821 cacaaagaaa tgcatacaga agtagaatat attattgctc atggcatttc aacaaaagtt
     5881 gatgataaaa aggttgtaat aggtagcaaa cattttgtat ttgaagatga gaagtgtact
     5941 atcccagacg gtgaggagga taagtacaat aatctatcag atgagtattc acacctattt
     6001 atggcaatat caggtaaatt atcagcagtt atttgcataa atgacccttt acgaaaagaa
     6061 gcaaaatatg taatttcaaa tttaagagaa tgcggaataa aaaagattgt gatgatgact
     6121 ggagatagcg aaaaaacagc taaatctata gcaagtaaag ttggtgttga tgaatattat
     6181 tcagaagtct taccagaaga caaagccaat tttgttaaaa gtgaaaaact caaaggtcgc
     6241 aaagtaatta tgattggtga tggaatcaat gattctcctg ctatttcaga atcagatgtc
     6301 ggaattgcaa tgagtgaagg tgctgaaata gcaagagaga tttcagatat taccatatca
     6361 gcagataatt tgaataattt aatcgtttta aagcagatta gtaataagct tatgaaaaga
     6421 gttgatttaa gttctaagtt tattatcggc tttaatttag gtttgatttt acttggtgta
     6481 ggtggatttg ttagaccaag tacatctgcc tttttacaca acgcatctac tgtcggaata
     6541 agtttaaaca gtatgacaaa tttattggaa aacaccaata cttataacta ccattaatct
     6601 ccattacata aaacatatga tataaaaaat ttataattca tctttttaaa aaaatatgtc
     6661 ttaaaattag cttttttcta atcaaaatta tataaagcat actttacaaa ttttaaactc
     6721 aacttatttc tattttttac aaaaaaggag atgattcaaa ttttcatttt gaaacatccc
     6781 cttttgtaaa tttctttaaa atttatatat taattatatt ttcaaccaat aattgtattt
     6841 tcaaccaatc tttcttcata gcattttaaa tcattttgct ttagataatt aaattcagac
     6901 tctacataaa taccacagcc taccaattca attgtcaaat taggtatatt ttcctttagt
     6961 atactagtct gctcataaat aattctttcc aataaatcaa cttgtaatat agaaaaatgc
     7021 ttcataatct ccccatcact atctatatct ttcttgattt cataccaact ctttgacaaa
     7081 attcccttaa aatcaatata ccctagactc ttcaatatat ctatactatc gctccatgca
     7141 tacttactag gtttatcaaa gtatgactct agtttcttct tctgctcatt tgtaagtgaa
     7201 tcataagaaa gcttcttatt aaaatgtgcc ttcaaagaaa tcttagccat agaatatgga
     7261 cttctaccag agtttaatcc tgcatttttg aacttttcaa ttcctttatc ttctactgaa
     7321 gaccatattg agtttattga atctgaatca ttgtatttaa catcaagacc atgtgtaata
     7381 tttatatagt tttgtggaaa tacatattga taatagacat cttttttatc atcatataga
     7441 gagaagtatc cactatcata taaatgaata tgcaccttta cttttaaact atcctctaaa
     7501 atatttcgtc taaggttcgg gatatttgca tacatggtta catattttat ggcatcaaag
     7561 tttttattta atggaacttc aaaactaatt tcatctcctg tcaaaagctt tttcacttct
     7621 tcttcattac aatcatatag agaatatgta ttttcaaatc ccacacgtga tttcccatct
     7681 aaactgcctg ttaatgctac cttcaaaata tggttatctg ttttttgcca ttggtaagca
     7741 tcttggtcaa gaaactctct aatctgtcta ttttgctctt tcgcctgatt tataacctcg
     7801 ttttcatctt ctgtaatccc tacatggcat ctatttaaga tatttactat attgcttcca
     7861 aatgggaatg tccatagttg aaaagctgct gcctcaacct gacacaagta tctaacctga
     7921 tttaattcaa tttgatggat aagttttttc tgatattcat tcatctgatt accactatct
     7981 gacatttgat aaatcaattc tcccattgca agattatctt ttttcttatc aatcttatca
     8041 gtaatgataa tttgagtttc catcatatcc tgtacttttt taaatattat agaaatctcc
     8101 tctgaatttt ccatttttaa gcttccaaac tttgtgctta attcagttaa aacttccttc
     8161 atctttaatt tacttatctc attttcaaaa atatcttgat ttttaactgt actatcgaat
     8221 ttttctacaa atggtataac agattctttc aaagttttat ctttaaattc caccatttga
     8281 gtattcaatt catctaattt cttaatatta ttttctttat ctttaattgc ctctttaact
     8341 acctctattt tactctcaaa actagatttt acattaagac aattattaac accactaatc
     8401 gcagtattta taagagaaca agcagaactt ccaatactca atgcattttt aattttagat
     8461 gtcttttgct gtctttcaat agcttgttta cttttctcta gtgcagctaa cttatctttt
     8521 tgctgtttat tattgtaatt tgcctccaac atacctattt cttttttctg gatttttgtg
     8581 taaatttcct tatactcgct ttcacttaac ccctcttttg ctctttgaat atcgtcagct
     8641 ttgtctgcat aattatcatc atctatagac ttaactataa tctccttaat ttggtcaaat
     8701 ttagttcttc tttcttcatc aacacctgcc aaattataag tcattttatc taaagcattt
     8761 tttctatctt cacttagctg ttctccaagt acaaatctca taccatcaga atccaattta
     8821 aaatctgaaa aattaaccat cctgttagtc catttgtatt cctcttcttt agctgaaaga
     8881 ttttccttca aagattttgc actgtcaaat gcccctagac aagcttgacc aacaccacta
     8941 aatgcagtca gtaaactcaa tgcgctttgt attccagaaa gaattaactg tgttttcatt
     9001 tgttcctcaa gttttgcctg tttctttttt agttcctcta tgtcttcttg ttcatcttct
     9061 ttcatcttta taatttcttg aatcaacagg ttcatttttg cattaacaga aagcatttta
     9121 ttctcaagtt cattttttaa aacctctatt tgttgatttc cagattcaat ttgagttagg
     9181 atactttcct tatatgtatt gaaatatcct tcttttaact catctttctt cccttgttcc
     9241 aattctctta aggtcttatc cagataatct gtcaaagaac gaatatctgt aacacgattt
     9301 cctccaattt cactttcttc aatcatctgc acataaaatg ctgtcttctg ctcaagtgca
     9361 aataaaatca tactccattc gctaaaattc tgattttttc ctacaaaaga ttctattcta
     9421 tcagactgca tcaatttatt tataaggtat tcatagattt cctttgcttc tttataaaat
     9481 tctttctttg tattcatatc atccagagaa agtactgctt tatttttctt ctcaaaacta
     9541 tagtacttct gaagtatttc tctatttaat tgcatctcta ttttctcaac tgcatgaaaa
     9601 atttcataaa tatgtgaaag ttctaaaata tcagcttcta aaattcctac tgcacacaac
     9661 agtctaagat ttctataacc tttaattcca gaaaaaccaa atctctcgaa aaattcatag
     9721 aagaatgtat ctattgacaa agaatactcc tgctttaaca attccttttc ttttagtgca
     9781 tctccagagt gaccggctgt tccctctgct ccggctgaac ctgaatcttc acgtttgcta
     9841 attatccagt ccttatgttc ttcataaaga gcatttaatt tctctccatc atctgctgct
     9901 tgcttctgat ttaaagcatg attaaacaca tatctatcaa aataatagta gtttctatca
     9961 ctattatcca ttggaggaga ggtacctata tatcttattg tttcttcata tatacctcta
    10021 attccaccaa gacctcctgc accaccagct cctccagagc ctccttttcc tgctaaacca
    10081 tgatttccat cttttttact tatagcactt tttaataaag gagcttttcc aaggagataa
    10141 acctgtccag acttgcttcc aagacctgca tttccaccat tacctcctgt gcctccagtt
    10201 ccaccttgtt ttcctttttc tgcttttacc acatagtatt ttgtaccttt aacattagca
    10261 aattcttttg actctttttt agaacctatt ttagcatttg ccccagcttc tccagttatc
    10321 cctgtatctc ctttttttcc atcttgtcca tttccaccac gtccaccatc tacgtaaagt
    10381 ttaagttctc ctcttttttc tatttcttta aaagataata tgatatcacc tgaagtacct
    10441 gcattattgc catttcttcc attcattccg tcgtaaatag aactttctcc aaccaatttt
    10501 tctgcatcct taccagatac gttgattgaa taagtccctt ctggtatatg taatttttct
    10561 gtaagtagta caatattctt acctgagaag acttctttct ccatattaca gtcaattttc
    10621 aactcacaat ctccatacat atatatagat gacaaaatat taagttgata accagatatt
    10681 attttagtca aatccgataa attcacttta ggtataggaa tatatataga aagtacattt
    10741 tctttctcat atgattttat atatgtacaa ctttgaatct tctctatcct atactctgca
    10801 tcttcaagct ttttggcttc ttcttcccta tattcttctt ctgctatcca atatttgata
    10861 tcttttaatg tatcttctaa agtatttttt agcaatgatt ttaacaactc acttagacca
    10921 acaatatgag tctcactaca gtaactattt aactctaaaa gaagacgtgt ttcttctaat
    10981 atattcactt tctgtacgcc atcaccatat gaaagttctt gtacaaactc cataatctct
    11041 ttcatattat tagtcctact acataaatct gtatacatct tatctaattg aatcttttta
    11101 tcttttagac tatttataga agatagaatg gaaatagaat cttccatacc ttttttaaca
    11161 ctttctatat aaggaactaa aagtttttct aggtctttat aggttacatc aacagctttt
    11221 tgtatttcta ttttacaaga agcagtaagc atcaacccca aatctgtttt ttcaaatgtt
    11281 tcaggtagat ttttcatttc atccaacaac ttttctggct gtttatctag tggattacat
    11341 aatttaatag atacaccatc tgataatgct acatttaata agtacactaa ttccttacaa
    11401 ccgtcgtaat ctttctcttg actatctagg tcttcttttg ctttttccaa tatatttctt
    11461 attttttctt ctgttacatt accaattagt gttttaggta ttttattgat aagaaagaag
    11521 aaattttctt tatactttgc ataatcacca agcatatctt tcaaattttt gcctgtttct
    11581 atcaataatg tatgtctaga gtccagtagg gattgatagt ctaaaataac taccatccct
    11641 tttacctttg aagatgtgga aatagtttcc cttacaagca tagcaccagc aatcatatac
    11701 tctgctccac ggttatcaaa aaaacctggg caatcacata atgaaaaatc tctatttgca
    11761 atatctacaa cttctggaca taaagtctta gaatatacac catttccaat ttcgacagct
    11821 gaatcttttg cagttatata tatttgtcca gtctcttcat ccgttttttc ttccatcaca
    11881 cagcctttta aataatttat acaagtactc tttccaacgc ctgaagcacc taagaataac
    11941 aaaattgact tttcttttaa gtcatatttt ctaagctcgt ctcttccttc ttccaaaagg
    12001 tttacgattt cactataaag tttatttgcc atacatccat ccctttctat atttcggaac
    12061 aatagccttg atatttttct ttaataagat tctcttctac ctttgaaaca gctgttgata
    12121 acttagtgtt tggagcatta aatcctccat tgtctcctct atctccatat tcagcgtatt
    12181 ctcttgctgg tcctgtttta ccttcattcc agcttccacc actaaattct atatgatatt
    12241 taattatact atctaactta taatcccaac ttgtaaaata tcctgtatag tatacagtct
    12301 tatctatacc acttggagaa aatgaaagtg tattatatcc gtgatagcca ccacttcctc
    12361 catatccacc taaacctggt ctaccatttg gacctgcttc tccatctttc actgttatat
    12421 cacaatgatt aattattcta tttttttgtt cctctgaatc tgaaatgaag taaatattac
    12481 ctttcttacc agcatttcca cctcttccag catttccacc agctccacct ttttcacctt
    12541 ttttgccact tgtacctttt tcaaaatacc ctttaaacat ctgtccgtgg aaataacttg
    12601 tatatcctgc atcagctcca ttacttccag aagctcctgt ttcaccattt tccccatcta
    12661 ttcctttaga accatttcct ccatttgcaa tcagtgttag acgataattt ctaatttctt
    12721 ctttaatata taagtaaata ttacctgcat ctctaccatc ttcaccattt ttcccataca
    12781 tagaattatc tacaagttct ccgctctttc cacttacatt aatttttaca ttactaccta
    12841 catcaagatt gttagaagca agaataatat ttttctctga tgcaaagttc attccaaggt
    12901 tatcatccat ataaatagta tctttagcaa agaacataaa tgtagaaaca ttgtcatcta
    12961 cttttctatt tttgaaatat tccaatacac tgctaattac taagtttgga attaatgctc
    13021 ttatttcaat acctttctta tcattaatat taattgtttt tactataaat ggatttaaaa
    13081 tgtatttacc aataaattct ctaattatct cattaccttc ttctccatat gcacttaatc
    13141 ttgttatact gtcttctaac tcttcatttt gtgtaactgt aacatttgtc aaaattgaat
    13201 taaaatcact tacactatat acataatcct ttttatttgc aacttccatt aatttttctc
    13261 tatttggtaa tgtaaatacc tgtttttgca cttcataaga aactaaaact ttttctaaat
    13321 tctttcttat tataatgaag ttttgttcca ttatcttctt acttgccttt tctataataa
    13381 attccatatg tacagaaggt aataaaattt ccccagttcc atgttctgta tatttactta
    13441 aagcatcaat tatatctaag tttcctttta tcttattttg taatttcatg caactatctt
    13501 gtaagaataa tccactttgt ttggctaata cgttgataaa tctctctata gtaagacttg
    13561 ttgtactttc caaccagttt ttaaatactt ttacaacctc atcatatttt gctatttctt
    13621 gaatcattgt tatacttcca ataatattga taaaatcttc tgtatacgcc ttaacataat
    13681 cttctaggct agatacgata ttgttttgaa tttcttttat caacgtatct aaaactaatc
    13741 ttgcatctgc actaattgga tattggaatg ctgatgtctt atctttaact tcatctaatt
    13801 ctagtacttg ctttaataag ccttccctct catctgatgc aagtggctta caaagatgaa
    13861 tattttttcc attatctttt agcaattctt caaataacca agctttttcc tctatattac
    13921 tgtctaaatt ttcgcataat tgttcatatt ttctttttat aacattatat atttgttttt
    13981 ctgttcttcc taagaaactt tttgaaatga aaaaagttat actaccctta aaattttctt
    14041 tatgaagaca catatttaac tgttggaaca ggtctagtac ttttgtaagt ctagaatcta
    14101 atatatctgc ttctggtatc aatacaatta ctgcacatat agatgcagaa tttttaaata
    14161 cttcacctaa tgaaactgct gcactaatat catgggctac tcccctattg tcgctaaaac
    14221 ctgcagtatc acataatttt gcatccattc cttctattgg ttttctcata tttacaatgt
    14281 tcgggtataa agtttgagaa acagctcctg aaccaatctc tgccacaggg tctaagcaat
    14341 caatatatga tataccagta tcttcatctt ctacttccat cattacacat cctgcaagat
    14401 agttaattgc agtacttttg ccacatcctg tcccaccaag aattagaatt aaattttgat
    14461 tttcagcctt atttattgct acttttcctt catttaataa gagtgttatt tcttctaaaa
    14521 gttgattgtt attttctggc atttattttc ccccttggtt atatatttta tttaaataaa
    14581 gtttaatccc ttaagtagat tgcaaagttt ttatactata tttaaatttg ttttttattt
    14641 tttattttta ataaatttgc attttcacta aatttgtatt ttcactcaat aattattctt
    14701 ctttgtttat taaaataact gtgttgtata atataatctt tatcaacaca gttattttat
    14761 ttaatgcgct tattttattg tattttatgt aatacagttg ttttttatat tatatgtcca
    14821 ttattttcct gtggttatta catatagatt tttaattata tttgtaccat taatttaaac
    14881 attcctatac tactgcttaa gttttacacc tataccagct tgaatatttt atttttattt
    14941 gcttaaatag ttcaactata ctggtttaaa taggttatct ttatttgctt aaatagttca
    15001 actatactgg tttaaatagg ttatctttat ttgcttaaat agttcaacta tactggttta
    15061 aataggttat ctttatttgc ttaaataagt tcaactatac tggtttaaat aggttatctt
    15121 tatttgctta aattagttca actatactgg tttaaatagt tttcctttag tttaaataat
    15181 tcacctatac ctacttgaat agttcacttt tatttgctta aattctatac caatactggc
    15241 ttaaacatta tactaccatg attgtattgt ctatcttcaa ctacaataaa aacaggttct
    15301 aaatctttag tagatgttat ttttaattct aaaaaaacat tatcagttgg taatattttt
    15361 acatcataat tatttaaatt attattcgtg atactattaa atacatcacc ttgacgttca
    15421 aaaaaaacat tatcaactat tacaaaataa acatcttctt ttctactatt ttcaacctct
    15481 atagtatttt gtttagttac atttagtctc atcttccacc tcctattaat attacttaat
    15541 tatattaata cttgtaattg ttgcattatg ttcatcacaa ggaagaggtt taaaaacaac
    15601 tatctctgct aaataattct gtggaggatt tgtaactgaa gtagcattac aaataaattt
    15661 aaaatttgtt cctgctacaa cttgagtaga aacaataagt ggttcataac caaccccttc
    15721 taacattcct acagcttcat taaatacttt taaatcttct tcttcgacag ctctgaattt
    15781 tgaccatcca ccaacaaaaa gactcatata aaaacctcct ttatatatat atttatttat
    15841 atatatagta gtaccaattt tctcaatatt caatattttc tgtgtaactg gtcaatgaaa
    15901 atgcataact ggataaaaat acttataaaa taacttaact ttacatataa atagcttaat
    15961 ttacatgtaa taagattatt ctacaatatg aaatacatct tattttgaat atattctaaa
    16021 tacaataatg gatattttca attattatac ttagaaaata tccattactt aatttgtttt
    16081 atttaaaatc acatagagac tttattattt tttttattgc atgaatagct atttcaggta
    16141 tcataaggtg atataccccc tccttgaaac cacaaaaaac ataacaaact ttacgaatct
    16201 tgtcttaaaa gaccacaggt acattcctta tttgtggatt ggagtagcca taacaagcaa
    16261 attttacaat ttttatgatg acaaaaatca ataaaatcgc ttagtatata acacgaaata
    16321 ctacttgttg ctattaagaa tagttagata ggaagtatgc attataccaa taaaggaatc
    16381 aataaagaaa gttaaaacat ccctacaaat taaaaatctg ttacattttt ctatatttaa
    16441 acgttattta cttacaattg gagaaataac aggttttcct tctcggtcaa gtaaaggcgt
    16501 aaatccggct gcatggccat aatagtggaa tacatagttc acacctgttt ctttatccac
    16561 ccaaatctcc gttgcattta tttttccttg tgaatatact ttttcaaatc tatcttttcc
    16621 cattttataa tcctccatat ttttgattgg tctatgcctt cttcacttaa gtagaaaaaa
    16681 tacataactt atttttttaa ttctaatatg agcacgtaaa aatgtcaaac agcttattta
    16741 atttcataac ttcatgttac tgttcgtaac cttgtctgtt tacttacact gcattacagc
    16801 accattttat cctttacatt attgtattgc acacagtaaa aatgcttact ttattggatt
    16861 tttcaactaa aaaaaggatt ttttctccac agaacattta ctatttttta taatatatct
    16921 aattttactc tcatatcaac atttaatgaa gatataacct ttttcaaata ttgtaaagac
    16981 tccttaaaaa tattagaaaa agattctgca aatctctcaa catctccttt ttttaaatac
    17041 ctaagtatct caaaacatac atcaaaacgt gttttcaaaa tttcattata ttcttcatga
    17101 aaaaatctct ctaaatagat tccaaatatc atatattgtt ttaaatgtaa aaatgtttcc
    17161 tgtaatacat aaatttgtgt ataatctact atcttacaaa gtaaaacaaa atctggataa
    17221 accttacact cttcacaatt attttgtaag taccttaaat gagtttctat tttatctaca
    17281 gctatcaaat ccattttatt tcctagtaaa agtactacat cctcaagagt aatcgtaaca
    17341 aactccatag catccaaaag caaactcaac cttttagctc cttcttctgt atttatataa
    17401 tcatcatcat attcagaaaa aagtgttata tatgttccct tgccattaat tgttttagca
    17461 attcccaatt catttagctc attataggca cttctaactg ttggaagaga tacattatat
    17521 ctattaatta tcgaagtaat tgatggtaga caatctccta ttttaatatt gccttcaaaa
    17581 atttgacaaa ttacatctga aacaattaag tcatataaat aacatttttc caacgttaca
    17641 gaattaaaac tacttgtatc tatactttgt ggagcacgat tttctaccat tttaagtatg
    17701 gattctctac aagctttata catatatgtt aatagttttt taaattcaat tagattcccg
    17761 tccaatccat ataagtaagt ttttcttacc tgctctatgt atttattacg aaaaaagtct
    17821 atacaacaat tatccaagtt atccaagtta tcgaaattat aggccaattg tgaaataatg
    17881 acatcatgct gaataatctc acaaaaatgg tgaatcagga aattatttgt tttacttatt
    17941 agtttattta tatattttag cctttgttct ttaaagacta aataattaga actatttttt
    18001 tccatacaat cgataatctc gtttaattct tcaaagtctt tagatgtaaa tcttttaata
    18061 ttgtacatag caatagaagg aaatattaaa ctaaaaatct tatatatttc tgatatttgt
    18121 aaaatcttct catataataa gtcagattct attgattgtt tactcgtact ctggattaaa
    18181 actggtctag aaccagcttt tgtctctata aagccctttt ctgagagcat ttttattaca
    18241 gtttttatag tattttttga taccatatat tcttctgaca tgctttttat agaatcaagc
    18301 ctatctccaa tagtaatttt accagtataa atttgttgaa ttatatgctc aaaaataatt
    18361 aaatattgtg gttttctctt catatttttc ctcttcgata aattagattt attgtttttt
    18421 gtaaggtata tttactttta tttaataggg caaaatagtt gcttattttt tcctcataat
    18481 ctataattaa tatagaccat ttatatatat ggtctaagtt ttataatact gtaatgcagt
    18541 aaaatagcat attgtagcta tactacagca aattaacata ccattgtagt atattaattg
    18601 gaataatttt aagtacttca aaattccagc aaaacatata tgtgtttttg atattatgta
    18661 tattcgttaa tagaatagct atacttgttt tacataataa attatatgat acttgctata
    18721 tgtaattact atatttaata cacataacta ttatacttac tatatgtaat tactatattt
    18781 gatacacata attattatac ttactatata taattactat acttggacac ataactatta
    18841 tacttgctat atgtaattac tatactttat acacatagtt attatacatg ctatatataa
    18901 ttactacact tggacacata actactatat ttactatatg taattatact atacgtggac
    18961 atatagttat catacatact atatgtaatt actatacttg gacacataac tattatattt
    19021 gctatgtgta attatactat acttggacat atagttatca tacatactat ataattacta
    19081 tacttggaca cattatcata catactatat gtaattacta tacttataca catagttatc
    19141 atatatacta tatataatta ctatacttgg acacatagtc atcatacatg ctatatgtaa
    19201 ttactatact tatacacata actattatac tttccataca taattactat atttggacac
    19261 atagttatca tacatactgt atattaagat ataatacttt ttaaatttta tataacacag
    19321 acacatgcaa taaaaaagaa ggtatccata ataagctctt tgcatgtacc taaataaaaa
    19381 ctgtaaaata gaatatataa aatctaatct attttaagta aatacaattt cactttaaaa
    19441 gaggatgtac tatgaaatac catatatttt taatcataac tactatatta ataatcgaac
    19501 tatgtaacat ttacatatgc aaaatagcat cttttagaaa caaaaatata actatacata
    19561 agaattacaa atatattttt ttaataaaac cattaacctt cttgataata atattcacaa
    19621 aattaccatt accactatat tatatattgt tttacatagt aagtcataaa ttgctcttaa
    19681 gcagaaaaag ttctactaac aaccatttta ttcccaatat ggcagtaata agtttttgta
    19741 ttttacacct tattactgta agtacaatat cacttgttaa aaatacaagt ccagatataa
    19801 tatttgcaaa tatgacctat aagttaatta gtattttaat agcttctatt ttagatttta
    19861 tcttatggta ctctatgaat aaaataaaag agcatattta tataataact caaaatcaaa
    19921 tagagttcag tctattttta aaatttatct ggttcacttt aggatatata ttatttgata
    19981 gtatacaaat tacatttgct ccaccaagtt ctctaacttc ctactttata ttgggaagta
    20041 acatattatt aatcttacaa ctaattatat tcatcaatca tatatattcc attgaaaaaa
    20101 atatttattt ggaaaaagaa cataatagac taaaaatgat aaaacaagaa caattactaa
    20161 aagtaaattt attaaagaaa aaagccaaca ttgatgaatt aacatcagca tataatcgaa
    20221 aatttgcttt tgaaaaaata cagcattaca ttgataacaa tttattattt tcattgatat
    20281 atatcgattt ggataaatta aagacgataa atgatacata tggacattta gctggggata
    20341 actatattat taacttctca agtatagtta ttgaaaactt aggaatagat gacatatttg
    20401 ctagaatagg aggagatgag tttatagtta ttatgcctaa atctaatgaa aacaccacaa
    20461 aagaatttat agagaaaata agtaaaatta tggataatac gatatctttt agctatggta
    20521 ttgtagaagt ttctagcgaa aacaaaatga ctgtagaaga aattattaat attgctgata
    20581 ttagaatgta cactaataaa caaaataatc ataaaaggaa caataactaa aatgaatatt
    20641 tacatgagct tacttgtagt ctcactatgt tttttatctt attatctcta tcattataat
    20701 ttttttaaat atctggacat aatgattaat aactctaaaa ata
//
LOCUS       DAMJWU010000061        20722 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMJWU010000061 DAMJWU010000000
VERSION     DAMJWU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.061"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            60..1016
                     /locus_tag="PC460_002412"
     CDS             60..1016
                     /locus_tag="PC460_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF2601293.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            1193..1348
                     /locus_tag="PC460_002413"
     CDS             1193..1348
                     /locus_tag="PC460_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601294.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            1478..2491
                     /gene="aroF"
                     /locus_tag="PC460_002414"
     CDS             1478..2491
                     /gene="aroF"
                     /locus_tag="PC460_002414"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2601295.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            2513..3577
                     /locus_tag="PC460_002415"
     CDS             2513..3577
                     /locus_tag="PC460_002415"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF2601296.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            3577..4890
                     /gene="aroA"
                     /locus_tag="PC460_002416"
     CDS             3577..4890
                     /gene="aroA"
                     /locus_tag="PC460_002416"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF2601297.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELNKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            4868..5947
                     /gene="aroC"
                     /locus_tag="PC460_002417"
     CDS             4868..5947
                     /gene="aroC"
                     /locus_tag="PC460_002417"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF2601298.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            5950..7146
                     /gene="pheA"
                     /locus_tag="PC460_002418"
     CDS             5950..7146
                     /gene="pheA"
                     /locus_tag="PC460_002418"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF2601299.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRFIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            7193..8002
                     /gene="aroE"
                     /locus_tag="PC460_002419"
     CDS             7193..8002
                     /gene="aroE"
                     /locus_tag="PC460_002419"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2601300.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            8014..8547
                     /locus_tag="PC460_002420"
     CDS             8014..8547
                     /locus_tag="PC460_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF2601301.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDINMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            8568..9407
                     /locus_tag="PC460_002421"
     CDS             8568..9407
                     /locus_tag="PC460_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF2601302.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            9675..11156
                     /locus_tag="PC460_002422"
     CDS             9675..11156
                     /locus_tag="PC460_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2601303.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVYDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            11189..14626
                     /locus_tag="PC460_002423"
     CDS             11189..14626
                     /locus_tag="PC460_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2601304.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLITYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVELGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            14644..15390
                     /locus_tag="PC460_002424"
     CDS             14644..15390
                     /locus_tag="PC460_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF2601305.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(15339..15647)
                     /locus_tag="PC460_002425"
     CDS             complement(15339..15647)
                     /locus_tag="PC460_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2601306.1"
                     /translation="MNFLKENSTFYFATVEDNKARVRPFGFIMDYEGKLYFGIEKYKT
                     SYKQLLVNPNIEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            16450..20583
                     /locus_tag="PC460_002426"
     CDS             16450..20583
                     /locus_tag="PC460_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2601307.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISPPIGST
                     NLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDADNGTF
                     GFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSNVIGA
                     WWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVTGYPP
                     PQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFSEGES
                     AIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTNVFFR
                     DLIPNGLEFIPGTVTVDGVSVPGVDPNTGFSLPDIGGGTGVEVTFDVVVESIPNPNPT
                     NNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEELIYTI
                     KVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETRVIII
                     RGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNSAVPG
                     TLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGTLNSG
                     ISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISIIKVAD
                     EDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWNGSLN
                     IGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSADIEV
                     IKTSNMDTAVPEETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVTFQPW
                     NGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDETDIS
                     PLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYSIDNG
                     VTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNNNTST
                     SEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPASILSP
                     EYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPN
                     PDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTLIDNV
                     SDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNIAEVT
                     STTPDSNLNNNISRVQVEIKQFCCNDCGCKDCCCNCCCNDCGCKNCCCNCCKNDFWC"
BASE COUNT         8436 a         2262 c         3446 g         6578 t
ORIGIN      
        1 attacagtaa ggattataaa aaatttttag aatgtaaatt gaaatttagg agtagatgta
       61 tgtatatact gaatttaaat acaagagaga ctattgaaga ttttagagat aaattttatg
      121 tagcagaaaa ttcttatctt atattaagtg ctccaaaaaa tttaaaattg cttaaggaaa
      181 ctttggatat agatgaaata acttttaatg attgtcttaa atttgatgaa ataaccaaac
      241 ttgatttatt tgataattac gattttttaa gcctaaatac atttgaacta agagatggag
      301 aagctgttat agaagaggta aatatgtatt tatcagataa ttttatattg gttgtagtga
      361 atgaagaaca ctttttattt gaatttgtaa aaaatattat tttgaagaac tctcaacttg
      421 aaaaaaatcc agttattaat ctatttaaga taaactactt gattcttaga gaagttataa
      481 aaaatggttt tgagtctttg gaaaaagttg aagagttaat tcttcaaata gaagatgaaa
      541 tgatggacaa cattaataag aatcatgtat ctagaataag tgatgtaaga ggattaacaa
      601 gaattatagt taagaatact agacctcttt tgtatattgg ggatagaata gttaaggaaa
      661 atataagata tttaaaatat tcaaatgtaa aaaaatataa tttagaaaat tttcaaggta
      721 tagattttgg aattgacaaa ttatacaatt tcgcattgtc aacacgagag ttagcagata
      781 aattattaga tatttattct tccagagtag gtgagaaaac aaataactta attacaaaat
      841 taaccttgtt aacagccata tcagcaccac ttacaattat aactggtata tatggaatga
      901 actttagata tatgccagaa ttaaactgga tttatggata tccagcaact ttatttttta
      961 tgctatgtat aatttttgtt ggaattataa tatttaaaat aaaaaaatta ttgtaaatta
     1021 cagatgatgt ttaattgatt ttataaaaat acatcaattg agaagctgat ttaagtaaat
     1081 aaaaaaatat tttaaattga aaatatttat agaaaatatt gacaattata ttttctttgt
     1141 tataattatt aaacaagaag aataataaat tataaaaatg gaggataata ctatgaaagg
     1201 aatattaaaa gaactaacag caaaacttta tagctttttt tactttatgt tctggggctt
     1261 ttattttagc gctggctatt tcttttacaa aaatatatgt acttcctttc tggtgaggcc
     1321 ttattttagt gattttgagc ataagtaaat tttataaaaa tgattatttt atatatttat
     1381 ataagattta gtgcttgata gattatttta gagaactcat tagtgagttc tcttttttat
     1441 tttaaatata aaattataat attgggaggt agtaaaaatg atagtagtat taaaaatggg
     1501 agcagataaa aatgaagtta aaaaattaat tgaggctatt gggagagaag gagtagaagt
     1561 taatcccata gatggaacag aattaactgt tttaggttta gttggagata ctagtaaaat
     1621 agatgccaaa agaatagaag caaataaggt agttgaaaaa gttatgcatg tggtagagcc
     1681 ttttaaaaag gccaatagaa aatttcatcc agaaccttca atcataaatg ttaatggaat
     1741 ggaaattggt tcaaagaaaa ttgctatgat agctgggcct tgttctgttg aaacagaaga
     1801 ccaaatagta tctatagcta aagatgtgaa aaaatcaggt gcaggatttt tgagaggtgg
     1861 agcattcaaa ccaagaacat caccatacgc tttccaagga ttgaaatatg atggattgga
     1921 tttattaaaa aaagcaaaag aaaaaacagg gcttcctata gttacagaaa taatgtcaac
     1981 acaagatata gatatatttg aagaaaatgt agatgtaatt caagttggag ctagaaacat
     2041 gcaaaacttt gatttattaa aagaattagg taaaacaaat aaaacaatac ttttaaaaag
     2101 aggattatct gctacaattg aagagtggct aatgtcagca gagtatataa tggctggagg
     2161 aaatgaaaat gtagttcttt gtgaaagagg tataagaact tttgaaacat atactagaaa
     2221 tactttggat ttaagtgcaa ttttagctgt gaagaaactt agtcatttac cagttatagt
     2281 tgacccaagt catgcagcag gtaaatcatg gatggttgac tctttatcta aggcagcaat
     2341 tgcagttgga gcagatggtc taataatcga agttcataat gacccagcac atgcactttg
     2401 tgatggaaaa caatcgatta agccaaatga atatgatgaa ttgataagtg aattaaagac
     2461 tatcgcatca gcagttggta gagaaatata atctataaaa ggatggttaa acgtggaaga
     2521 aaaactatta gtaaatttaa aagaaaatag ctacaatata tttattaaaa aaaatatact
     2581 aaagaatata ggagaagaaa taaaaaaaat atattttgga aataagattt ttattgtaac
     2641 tgatgaaaat atagcaaaat tatacttgga taaagtaaaa aataatttaa gtgaatctgg
     2701 atttgaaatt aaatctttaa tattaaaatc tggtgaagaa acaaaatcat ttaatactct
     2761 tcctaaaata tacgatagtt tacttgactt taagttaact agaaaagatt tgattattac
     2821 tttaggagga ggggtaatag gtgatttagg tggatttgca gcagcaactt ttttaagagg
     2881 tataagtttt attcaaattc caacatcttt attagcacaa gttgatagtt ctgtaggtgg
     2941 aaaagttgga gttgaccttg agagaggcaa aaacttagtg ggaagctttt accaaccaaa
     3001 ggctgtgttt atcgacccaa atgtattatt atcacttcca gaaaaattct atagagatgg
     3061 aatggcagaa gttataaaat atggatgtat aaaagacaaa acttttttca ataaactttt
     3121 aagtttaaaa aatagagaag aagtaatgca acatatagaa tacattatac ataaatgttg
     3181 ctttattaaa aaaactgtag ttgaagctga tgaaaaagac tttggagata gaatgcttct
     3241 aaactttggc catacattag gtcatgctat tgagaaatac tacaatttta cgggttatac
     3301 ccatggagaa gctgttgcca taggaatgta taagataact ttagaaagtg aaaagatagg
     3361 aattacaaaa aaagggacat cagaacttat aaaatcaatt ttaatgaatt ataatttgcc
     3421 ttatgagatt gaattaagag ataataaatc tatagtagat gctatatctc ttgataaaaa
     3481 aaatctaggt gatacattaa aaataatact tttaaaagat attggagaga gtattatata
     3541 taatactact tctaaatttt ttcaggaggt aagataatgg gaagtctaaa aatatatcca
     3601 agtaaattat ctggagatgt aaaaatacca ccatcaaaaa gtatggcaca tagggcagtt
     3661 atttgttcat ctctaagcaa tggaaaaagt agaatttcta atatagattt ttctgatgat
     3721 ataatagcta ctataagagc aatgacatca ttaggagcta ttatagaaaa gaaagaagat
     3781 attttagaaa tttcgggaat tttttctaaa gaaggtattt taaatagaga gaatcagttg
     3841 aatcaaccta aattgacaat agattgtaat gaatcaggct ctacattgag atttttagta
     3901 cctatatctt tagcatttga tggtgtaaaa aggtttatag gtagaggaaa tcttggaaaa
     3961 agaccacttg atacttatta tgagatattt gacagacaga atataaaata tagttacaaa
     4021 gaaaatcagt tagatttgat tatatcaggt aagcttaaac cagatgaatt tagagtaaaa
     4081 ggaaatataa gttctcagtt tatcacaggt ttactgttta tattgcctac gctagagagt
     4141 gattcaaaga taataattac tactgagcta gaatctaaag gatatttaga tttaactctt
     4201 tcaacgataa aagattttgg ggtagaaatt ataaacaata actataaaga atttattata
     4261 aaaggaaatc aaacgtataa agcgagagat tataaagtag aaggagatta ttctcaagga
     4321 gcattttatt tatcagcaga tgctatagga gaagatatta gcatattaga ccttaaagaa
     4381 gattctcttc aaggggatag tgaagtagtt gaaattttat ctaggatggg aatggaaata
     4441 ttaagagaag gcaacaaaat aaaaggaata acaaatggtt taaatggtac tttgatagat
     4501 gcatctcaat gcccagatat aatacctgta ttatctgtag tagcatcctt aagtataggg
     4561 aggactacaa taattaatgc tggtaggctt agaattaaag aatgtgatag acttcatgca
     4621 ataaatgttg agttaaataa acttggagca aatatagagg agaaagagga tagtttgata
     4681 atcgaaggag taagcaagtt aaatggtgga gtagaggtgt ggagtcacaa agaccataga
     4741 attgctatga cacttgcaat tgcttcttgt agatgtgata aaccaataat attaaaagat
     4801 tttgagtgtg tatcaaaatc ttatccacat ttttttaaag attttaagat gttaggaggt
     4861 cgaattgatg agtggaatat ggggaaataa tttaaaagta tcaatttttg gagaatctca
     4921 tggaaatgct ataggtataa atatagatgg tcttccttcg gggattgaac ttgaccttga
     4981 taatattgat aaagaaatga agagaagagc acctggaaaa aattctatat ctacttcaag
     5041 aaatgaaagt gatataccag aaattctaag tggatatttt aatggaagaa ctacaggaac
     5101 gccattatgt gcaatcataa gaaatagtga tactcgttca aaagattatg gagagcttaa
     5161 aaacttaatg agaccaggac atgcagattt cacaggtaat gtgagatatt ctggatttaa
     5221 tgactataga ggtggaggtc atttttcagg taggataaca gctccattag tattttgtgg
     5281 ggctatatgt aaacaaattt tatcacaaaa aggaatagag ataggagcac acataaaaaa
     5341 aattaaaaat atagaagata tgagttttga ttatgtaaat atttcaaaac aacagttatc
     5401 aaatttgcaa actttggaac tacctttact tgatttatca aaggaagaag ccatgaaaaa
     5461 tacgattata gatgcaaaaa atcaaggaga ttcagttggt ggtataattg agtgtgcagt
     5521 agtaggtatt aatgtagggc ttggaaatcc attttttgac tctgttgaat ctactttatc
     5581 acatttattg ttttctgttc ctgctgtaaa aggtgtagag tttggtttag gttttgaact
     5641 tgcagatatg tatggctctc aaagtaatga tgaaatgtat tatgaaggaa atcaagttaa
     5701 gagcaaaaca aataacaatg gaggaattat aggagggatt actactggaa tgccaataat
     5761 tttcaaagtg gcaattaagc caactccatc aattagcaga caacaaaaca cagttaatat
     5821 aaaagataaa aaggacgata ttttatatat taaaggcaga catgaccctt gtatagttca
     5881 aagggctata cctgtaatag aagcagtaac agccattgga atttttgatt tgatgaaagg
     5941 acgataataa tggaggattt aacgagatat agaaatgaga ttgacaatat agataaagag
     6001 cttattcaat tatttgaaaa gagaatgaat acagttcttg aaattgccag acataaaatg
     6061 aaaaataaca ctactatact tcaaaaagat agagaagaaa aagtactaag tagagccgta
     6121 gataatttag ataataaagc atattcacaa gaaacggtac agttttttaa ttctataatg
     6181 gaaattagta gaggattaca aaaaagattt atagataatg atggtgagca gaaaatcgag
     6241 tttagtttag attcatcaaa taagaatttt gatttgtcaa atgtaaataa atataagtct
     6301 ttaaaagatg aattaaataa aaaaaatatc ctagtaggct ttcctggaaa atcaggttca
     6361 tttacagaag aagcactaaa taaattcttc aacaaaaaaa tatcaaaaaa acaatttaaa
     6421 gaatttgaag atgttttcat agcacttaaa aataaagaaa taaactatgg aataatcccc
     6481 atagaaaatt catctacagg agctatctct gaaacttatg accttttgag aaagtacggt
     6541 ttttatatag ttggagaaga atgtataaaa atagaccaaa atttaatagg gataaaagac
     6601 acaaagcttg aagatataaa agaaatatat tctcatccac aaggtatagg acaatgtagt
     6661 gaatttttaa aacaaaatag tacatggaaa ttgataccct ttaataacac agcaacaagt
     6721 gcagaacttg taagaaaact tcaagataaa acaaaagcag caattgcaag caaaaaagct
     6781 gcaagtattt atggattaga aataataagt ccttgtataa atgatatagc aaacaactat
     6841 acaagatttg tagtaatcag taatcaaata cacattgaag aagaatcaaa caaaatgagt
     6901 gtagtatttt ctgttgaaca tgaagcagga aaactttata aagtacttgg atactttgct
     6961 aaaaataata taaatatgac aaaaatagaa tcaagaccta tgaaaaatgc ctcatggaga
     7021 tatttctttt atatagattt tgagtgttct atatacaatg ctaaagtgta cgatttatta
     7081 gaattgatag aacataatac agcatatttt aaatttatgg gagtatataa aaataaaact
     7141 gaatagataa gataaatttt tatttaattt ataattatta ggaggtgtaa gtatgaattt
     7201 tttcggtcta gtaggtgaaa aactctctca cagtgtttca cctcaaatcc acaagagagt
     7261 attcgagata ttgaatattg aaagtgctta taaaaacttt gaaatttcaa aagaagatat
     7321 atctaagcta gacggtgcta taaaattatt aggaatacaa ggggtaaatg ttactgtacc
     7381 atataaggag cgtattatga aatatcttga ttttatatca ccagaggcaa aacgtatagg
     7441 agcagttaat acaattttac tcagagaaaa tatgttatat ggttacaata cagattactt
     7501 tggtcttgat agtatgttta aaatggctaa tattgatgtt caaggaaaag tagcagttat
     7561 attaggaact ggaggtgctt ctaaagctgc tcttacatat tttattgatt ctggtataga
     7621 aaagctttat gtttcaacta gaaaaaagga tgataaaaaa ttattaaata gtaaagctat
     7681 attaatagat tatgaagaac ttaaacatat aaaaggagat ataatattaa atgctacacc
     7741 agtaggtatg taccctaatg taggaatttc accagtgtca aaatctatta tacaaaattt
     7801 tgatatatta atagatttaa tttacaatcc tggagaaaca gagtttttaa gaattggtaa
     7861 tagtatgggt aaaaaaactt gtgatggatt atatatgctg gtggggcaag caataaaatc
     7921 tcaggaaatt tggcaagata ctaaaataga taatagtatt ttagatgtta tatataatga
     7981 actaaaatta gaatttttgt aagggagatt gttatgataa ataaaacaaa agaaaaattg
     8041 atattaatag gtatgccagg tagtggaaaa actacaattg gtaaactttt ggccaaagaa
     8101 tataattgta gtttctgtga tatggatgat tatataattc aaatatctca aaaaagtata
     8161 gcagagttgt tttcagaagg agaagatatc tttaggaact atgagactca agcatgtaga
     8221 gaactttcta taagtgataa aacagtgata tctacaggtg gaggtgtaat aaaaaaggat
     8281 attaatatgg aaatactaaa agaaacaggt ataataattt ttattgatag acctatacaa
     8341 aagattttgg aagatataaa tattaattca agaccattat taaaaaacgg aaaagataga
     8401 ttgtataatc tttacaatga gagaataaat ctttataaaa aattttcaga tatagagatt
     8461 ttaaatgata aatcattaaa taatgctgta tataatatta caaatgcagt cagtgaaaat
     8521 tttaaatttg actttaaaga aaaataatct agggggaaat aaaaataatg aatattgtta
     8581 ttgtaggact aggagttata ggaggctctt ttgctaaagc attaaaaaaa gctggatatg
     8641 aaaatgtatt tggagtagat gtagatttag aaactttaaa aaaagcagaa aaagcaaaaa
     8701 tcataaaaaa aggatgtact acaggtaaag agttatttaa aaaagctgat ttaattatat
     8761 tatctatata tcctagatta gttgtagatt ttctaaataa taataaaaac ttttttaaaa
     8821 aagggactat aattactgat actacaggaa taaaagaaac acttatcaat gatgtgttgc
     8881 aaattattcc agatgatata gattttatat ttggacatcc aatggcaggt agggaaaaaa
     8941 agggtattga ttttgcaagt gaacaagttt ttaatggcgc aaattatata attactccaa
     9001 ctggcagaaa caacataaaa aatttagagt tagttgaaaa tttaatttta gagattggat
     9061 ttaaaagagt aaaaaagtta acttcacaaa aacatgatga gattatagct tttacttctc
     9121 agttacctca tgttatggca gttgctctta taaatagtga tgaagaaggt agagatacgg
     9181 gtaaatttat tggagatagt tatagagatt taactagaat tgcaaatatg aatgaagact
     9241 tatggagtga acttttttta ggaaatagag ataatttact aaaagttata gaaaactttg
     9301 aatcagaagt aaatttagta aaagaagcta tatttaacaa tgataaaaat aaattaatag
     9361 agtattttaa aaaatcatca ataagaagag aagctttaga aaaataaata ttaatattta
     9421 agctatatat agatgggtat aaatataagc gatagtgtat ttaattgtat taataaatat
     9481 ggattcaata taattaaata tgctattgat atatttgtac ccttaactat tgttatcttt
     9541 tagatgataa tgatatttat ttaaatactc tttattaaca atttataaaa cttaattgta
     9601 aaaaatactt attaatagta aaataaagtg aatcaattat attataattg ctaaagagag
     9661 tgaggtgtag ctatttgaaa aaaaatagat taatagcaaa gaatatatca atagcattta
     9721 tagtactatt cttttttagt gtatttacat tcttttatgt aggtaatatt aatagagtgt
     9781 tagaatatga aacaaatgac ataataacag ttacaattgc tggatggatt atactttcat
     9841 ttttattttt aggtattata atttacatat tatattcaaa agcaaatagt caaaaaacta
     9901 tagaaaaagt agcatatacg gattttgtaa caggttattc caactggaga aagtttgaat
     9961 tagatgttac aaatttatta aagaaaacat cacaaaataa taaatatgca atggtcatat
    10021 tcgatattga taaatttaaa gcaattaatg atatatatgg ccataaaaag ggaaatttaa
    10081 tattaaaaga tattgcagat actttaaatg aattgactga tatcaatgaa acttttgctc
    10141 gtgtaagtgc tgataatttc aatatattat taacatataa caaaaaagag gatataataa
    10201 atattatcaa aaagattatg gcaaataatg aacttgtaaa cttatctttt ggaatttatg
    10261 aaatcaaaga taaggattta tctgtaagtg tatatagtga tagagcttca cttgcaaaat
    10321 catcaataaa aaataatagt gatgtaaatt ttgcattttt taatgacaaa ttaagagaga
    10381 aattattatt tgaagataag atagaaaaag aaatggaata tgctcttgaa agtgggcaat
    10441 tcgtaatgta tttacaacca aaatacaata ttaaattaga taaattttgt ggaagtgaag
    10501 ccctagtcag atggcaatat acagaaaaag aggtcatata tccaggtgat tttattccta
    10561 tatttgaaaa aaatggattt attagaaaga ttgatatgta catattagaa caagcttgta
    10621 aagaaatccg gtctttattt gataaaggaa tttcaccttt acctatatct gtaaacttct
    10681 caagagttga tttttttaaa aaagatttta ttgaaaacat agtaaatata tgtgatagat
    10741 ataaaatacc atatagttta atagaaattg aaattactga atctagtatg tttggagata
    10801 ctgatactct atttaatgtt agtagaaacc tacaggatat tggctttata gttgcaatgg
    10861 atgattttgg ctctggatat tcttctgtaa atatgcttaa aaatatacca ttaaatgtaa
    10921 taaaattaga tagaggattt tttgtttatg ataaagatgt tgataagagt caaattgtta
    10981 taaaaagcat agtttctctc attaagcaat taggaataag ggttgttgct gaaggaattg
    11041 aaacaagaag tcaaatagaa atgttaaaaa aagcaaattg tgatattgtt caaggatatt
    11101 atttttcaaa accattacca attaaagaat ttgaaaaatt agtctataaa atatagtcag
    11161 cgagtcttaa atgggaggtg gtgattaagt gataagagca aaatcgttaa aaaatgtact
    11221 gagtattgtt ttaatatttt tatttacaat aatttctttt tctgattttg catttaaaac
    11281 taatgtccag gaagaagaaa atagtaaaat taatttaaga gaaatatccc tacaaagttc
    11341 caatgctata aaaataaaat tagatagttc tctatccttt attaaaagtg tggcaaatat
    11401 ggtttcttat tttaatacta tagataactc aaaaatagtt ccattattag ctaatatatc
    11461 aaaagaatct gactttcaaa gaatctctat tgtatctcct gatggaataa gtcataccag
    11521 tgatggcaaa attatcaatg tgtcaaagta tcagtatttt aagaaagcta ttaaaggaga
    11581 aagtaatata tctgaagttg tgaaatctcc aattgatgga aaagatactt ttgttatagc
    11641 tactcctgta tttaataaga ataacaaaat aattggtgtt ttatacggtt cttactacaa
    11701 tgataaatta gctgaattta tagatataac aagttttaat agtgaagggt acatagatat
    11761 atttgaatct agtggtcaat ttgttttaaa atcttcacat acaaacaaac tgattaagtc
    11821 aagtagtaac atatatgata tatttaaaaa agcttctttt gatgatggtt atagctataa
    11881 aaaaatatat gaagatacaa aaaataacaa gagtggcttt acctcatatg aatacaaagg
    11941 agaaaatcgt tatgctactt atacacccat tggtgtaaat gactggtatg taatttctgt
    12001 gataccacaa tatactgtaa gaagaaacat tgataaaata aatcaattag cattttctct
    12061 tattttcaag gtattcttat tgtttattat gcttataaca tatattattt ataaaaacaa
    12121 taaatctcag aaagatatac taaaagctaa caaagaaatc tcatctatta caaatagcat
    12181 tcctggagga gtacaaaagt gtaccctggg tgaaacatgc aatattaatt ttttaagtga
    12241 tggatttata gagttgtttg gttatacaag agaagaaata aaaacattgt ttaataatga
    12301 tttttatagt ttaatacata aagatgatgt tgaaagaata aaaaaagaaa tttttaaaca
    12361 actttacaaa aataaaactg ttgaattaga atataggatt ataacaaaaa atggtaatac
    12421 agtatgggtt ttagataaag gggagcttat aacagaacct gatggaaaag aatatttttg
    12481 ttgtgtaact gtagatatta ctagaacaaa acaattagaa caagagatgg aacaaaaaaa
    12541 tctagaactt gaaatgatat cagaaaatgt tttaggtggt ataattataa cagaatttaa
    12601 tgatgatttt actataaaat acgctaactc tggttatctt tcaatgattg gatatacacg
    12661 tgaacaatta gaaaatgaac ttaaatacaa agcttgtaat cttgtttttt atgaagatag
    12721 aaagaaggct cttgaaagta tacaacatca attaaacaca aaaggaatag ctgtatatca
    12781 acatagaata agaagaagag atggtaatat tatttgggtt tctataaaag gaaggcttat
    12841 tgttgatgat gatggtattg aaaaaggtat ttggattcta aatgatataa cagatttaaa
    12901 aaatgcagaa caaaaactta aaataaatga agaaagattt agaattgcat taaagcaaac
    12961 atcaaacact atatttgagt atgatataaa aacaaagtca cttttaaata ctagtaaaga
    13021 gcttgataaa tataatattt ataaaattac taaaaatgtt ccatattctc tagtagaatt
    13081 aggagttatt cataaagatt ttagtgatga ttttataaat atgtatgaaa atgtaataaa
    13141 tggagcaaaa aaagcaagtt gtgtagttaa gatgaaactt ccagatgaaa attatatatg
    13201 gaataaagtt accttgacta cgatttttga tgaagatggt aatcctgtaa gagcaattgg
    13261 tatacaggaa gatataagca aacaaaagga agcagagata agatacaatc aagaaggtaa
    13321 gtatcattca gctatattgt cggaagcaat gataacttat gaaataaatg tatcaaagga
    13381 tacttttatc aaaggaaatg aaagttggca aaaaccattt ggaattaagc ctgacaataa
    13441 ctattctcaa atggtaaaaa ctatagcaaa tagtattgta cataaagaca ataaagaaac
    13501 atttttaaat acatttttat gtgaaaactt attggatgca tataaaaata atgtgactga
    13561 aataaaatta gaatatcgaa gaatgaatca agaaaataaa atgatttggg tcatttgtac
    13621 aatgcactta ataaaagatt ctattacgaa cgatataaaa gcacttgctt atgttaaaga
    13681 tattgacaag caaaaaaatg aagaaattag attaaaatta aaagctgaaa gagatactct
    13741 tacaggatta tataataaag gtactacaga aaaattgatt aatgatttct tgaactctga
    13801 ggaagcaagc ttaggaatgc atgcattttt aattgttgat atagataatt ttaaatctgt
    13861 gaatgataat ttaggacatg tatttggaga taaagtatta tttgaagttt ctaaaaacct
    13921 aaaacctata tttagaaaag atgatatagt aggtagaatt ggtggagatg aatttatagt
    13981 attcttaaaa aatattcagt cttttgagaa tatcaaaaag aaagcagaaa aaatatgcga
    14041 tatatttaga aattcttata cagaaaataa aaagacatat aaaatatctg gaacagttgg
    14101 aatttcattt gtaccagaac atggaactgt atttgaagaa ctttataaaa aagcagacat
    14161 agcactttat tatgcaaaga gcaaaggaaa agataagtat gcactttata atgattgtat
    14221 ctaccaaaat gaacttgaaa ataatataag tgataataat ataaagttag cagataaatg
    14281 tgagaaatcc aaaccagtac ttaaagaaat tcttgattct attgataatt atatctatat
    14341 agtgaatcca aatacttatg aacttctttt tgtcaataat aaggtgtctc gtttggattt
    14401 agagataaaa ttaggagaaa cctgttataa atcactatta aaatctgata gaccatgtaa
    14461 taattgccct cttaacggat tagaagaaaa taagcattca aaattcaaaa tagagacaaa
    14521 tacatttggt aaaaatatca gtatagcaag ttggattaat tgcatagatg gagaatacaa
    14581 ctgtcttata gagtgtatag attcttcttt atacaaaaat aaataaaaaa ggactatgaa
    14641 gatatgttaa atagtataac tttgatactt ggtataatat ttacaattta ctatttttat
    14701 ctcaaaatag tttttggagg aatttcattt agtgagattt tcttagttgt aggaattgta
    14761 ctaattatct atcaaatatt taagaaaaaa attaaagaaa agaaggtttt ttatagggtg
    14821 ttaaaaataa taatcagcat ttttttaata gtatttgtag ttactgagag tctaattatt
    14881 ttttatccca aaaatagttt agaatcaaaa agtgattatc tcttaatttt aggagcttct
    14941 gtaaaaaaga ctacaccaag tacaaccctg aagggtaggt tagacactgc attgaaatac
    15001 ctaaaaatta atgataattg ttatgtggta gtatctggtg gtaaaggaag tggagaaaaa
    15061 ataactgagg caaaagctat gaaagattat cttataaaaa atggtataga taaaaatagg
    15121 ataatagaag aggataaatc cacgaatact tatgagaatt ttaaatattc aaaattgaag
    15181 atagaagaac atagtcaaag aaaactaagc gatttaaaag taaaaatagt aacaacagat
    15241 tttcatgttc tcagaagtaa aatattagca tatagaaatg gatataaaaa tacgtctttt
    15301 tatgctagta aatcaaaact ttcatttgta ccaacttact atacaagaga attttttgca
    15361 ttatggaaaa caatcatttt tgatagataa taaataaaaa gactagatat aaactcatat
    15421 ctagtctttt ctattccctt aattctaatc cattttccaa ctttacttgt tgtagaaatt
    15481 tcaatattgg gatttacaag taattgctta taagaagttt tatatttttc gattccaaaa
    15541 tataattttc cttcataatc cattataaat ccaaatggtc ttactcttgc tttattatct
    15601 tcaactgtag caaaatagaa tgttgagttt tcttttaaaa aattcattat tttttcatga
    15661 ttaaatcctc tttgtatctt atactaatat aatgaatata aataaaatat tcattatact
    15721 taatataaat cgatataaaa acaattacaa tattatattg attattcgaa attatatata
    15781 aaattcagaa aaaattttag tttacataac aaaaataaag tcaactaaaa ttattgattt
    15841 tagattaaag taccggatta agcatttaag tagattttaa gcttgatttg attggaaagg
    15901 tacaaactat caccgaaaaa aattaaaccc atttaaaaag caatataaaa gcttgttatt
    15961 tttgagaaaa atagaccctc taaactcaat tagattaaaa ataaaataca gaaaattata
    16021 tctctaaata tcttaaataa ttaagtttaa attttacgat attttgtttg attatttata
    16081 atataaaagc atactcttaa tcttcaaaat ttaaaaagag taaaaaataa caaaaaaatc
    16141 actgacattt cactgacaaa ttgtaatttt gttattataa atacattgaa aatacttgat
    16201 tattgaaaaa tggcataaaa taatacattt tatgccattt actattattt gatagttttt
    16261 aacttatgac ataagatatt ttttaacaca tacaataaaa tataaaaaaa ttgatattgc
    16321 aaataaaaaa aatacatatt agatgttata gaaaaaaagc gcacttatag gcttaaaatg
    16381 tcacataaaa gagttttaaa gaataatata ttatgaacac aaattttgta atcacaaagg
    16441 agtgataata tggcaacagt atcaggacaa atagtttttg atagaaatag aagtgctacc
    16501 attgattctg gagactcagg aatagccaat ataccagttg tattacaaga tatagcaact
    16561 ggagttagac ttgtggttct cacagatgca tcaggaaatt atgtttttac taatgttcca
    16621 aatggtaact atagaattgt agaagctttt ggaactacag gaggagtacc aacaccaggg
    16681 aattttgcaa cagcgatagt aggtccagtt ccaattgcag aaatgccacc tattagtcct
    16741 cctattggtt caacaaactt agatggtaca acacctaaca cactattaat aacagtagca
    16801 ggttcagata taacaaatca aaatatatta aatgggcctg tgatttatac acctatagag
    16861 aatatattgg attcttgcgt atctgtatct aatataaacc ttattacaga tgcagataac
    16921 ggaacattcg gtttttttcc accaggtaca ccaagtaata caggacctga aatagaacct
    16981 tacccaaatg tgacgccaga ttttacgtat gttgtaccag acccaactaa gtttacacct
    17041 ttagatggag aatatacagt acaaaatatt atgacaaatg caatgagtaa tgttatagga
    17101 gcttggtggc gtattgctga ccatactaca ggaaatgaaa caggaagaat gatggtggta
    17161 aatggtttta atcctggagc tgtatttttt aaatcaacag tacaagttac acctaacact
    17221 aattatttat ttagcacttg gattttaaac ctttttaagg taacaggata tccaccacca
    17281 cagctaggtg taagaatatt tgaccaaaat aataatatac tatatcaagc aacattagga
    17341 gctcaaattc cagttaatac aaacgcacca gaatggaaag aagtaggtac tgtgattaat
    17401 tctcaaaata ataatcaact tacagtagaa ttttttagtg aaggagaaag cgccattgga
    17461 aatgattatg ctatagatga tgtttcttta agagaaatag ctgttccaac ttttactcca
    17521 ataaaaacaa gtaacacaga aactgctaat gtaggtgata taatcacata tacagttaca
    17581 ttagataata cttgtgatag ccctttaact aatgtatttt ttagggattt aattccaaat
    17641 ggtttagagt ttatacctgg aactgtaacg gttgatggag tatctgtacc aggagtagac
    17701 cctaatacag gtttctcact tccagatatt ggaggaggaa caggggttga agtaactttt
    17761 gatgttgtgg ttgaaagtat tcctaaccca aatcctacta ataatatagc taatatagat
    17821 tactcctata cacctgtaga aggcggtata ccaaatgatt tttcagtaga ttctaatcca
    17881 gttcctgttg aagtaatatc agcagatatt gaagttacaa aattatctga acctactata
    17941 gtgaatccag gagaggaatt aatatacact ataaaagtag taaataatgg accattccca
    18001 tctgaaaatg tggttttaac agatgatgta ccagcttcta ttataaatcc agagtattca
    18061 ttagatggag gagtaacttt tcaaccatgg actggaagtt taaatatagg gacattagaa
    18121 gtaggagaga caagagttat tataattagg ggtattgtaa atccatcaac agtaggaatt
    18181 ataacaaata cagctgtagt atcttcaact acagcagacc caaaccttaa taataataca
    18241 tctacaatag agacagaagt gaatttatta gcagatattt tagtaatgaa aacagcagaa
    18301 cctaattcag ctgttccagg tactttattg agatatacta ttcaagtaga aaacttagga
    18361 ccagcaaatg ctgaaaatgt aatattaaat gatgatattc cagcttctat tataaatcca
    18421 gaatattcat tggatggagg agcaagtttt caaccatgga atggaagtct taatatagga
    18481 actttaaact ctggaatttc attaacagtt ttaattcaag gaacagttag tttaaattct
    18541 tcagaatata tagtaaatac agcaacagtt tcttcaacta cacctgaccc agatttaagc
    18601 aacaatatat ctaccattat tacaccagtt aacccacaag caggtatttc tattataaaa
    18661 gtagctgacg aggatgtagc tgttccagga gaagaatttg tatatacaat tgaaattttt
    18721 aatgaaggac catctaatgc tacgaatgta gttttaacag atgatatacc agatgttatt
    18781 ttaaatccag aatattcatt ggatggagga gcaactttcc aaccatggaa tggaagtctt
    18841 aatataggta cagttgcacc agggcaatta ataagaatta tcataaaggg attagtaagt
    18901 tcaactgcta caggagatat tactaataca gcagaagtaa gtgcagatgt accagaacca
    18961 gttacggatt catcaacagt gacaactcct atagttccat cagcagatat agaagtaata
    19021 aaaactagca atatggatac agcagttcca gaagaaactt tcagttatac tataacagtt
    19081 acaaatttag gaccatctgc tgcacaaagt atcgttttga cagatgatat accagatgtt
    19141 attttaaatc cagagtattc attggatgga ggagtaactt tccaaccatg gaatggaaat
    19201 cttagtatag gcactttgga tgctggagaa ataagaagta ttataataag aggaacagtg
    19261 agccagacag cagtaggtac aattataaat acagcaacaa taagttcacc tacacctgac
    19321 ccaaatccag ataataatac ttcaacagat gaaacagata taagtccatt agcagatatt
    19381 tctgtgataa aaggcaacga accagttgct ataccaggag gaagatttat ttatggaatt
    19441 gaaattgcaa atgcaggacc atcttttgca gagaacgtaa ctttaacaga taatatacca
    19501 gcttctattt taaatccaga gtactcaata gataatggag tgactttcca accatggaat
    19561 ggaagcctta atataggtac tttagatgct ggagagataa gaaatatcat aataagggga
    19621 acagtaagtc aaacagcaat aggtacaatt ataaatacag caacagtaag ttcaactaca
    19681 cctgacccaa atctaaataa caatacttca acatcagaag cagaggtaag ttcatcagca
    19741 gatatttctg tagtaaaacg aagcaatcag actgttgttg taccaggaga tgtactagac
    19801 tatacaattg aggtgagaaa tgcaggaccg tctactgctc agaacgtaac tttaacagat
    19861 aatataccag cttctatttt aagtccagaa tattcaatag ataatggagt gacttttcaa
    19921 ccatggaatg gaagtcttag tataggcact ttagatgctg gagagataag aaatatcata
    19981 ataaggggaa tagtaagtca aacagcaata ggtacaatta taaatacagc aacagtaagt
    20041 tcaactacac ctgacccaaa tccagataat aatacttcaa catcagaaat agatataagt
    20101 tcatcagcag atatttctgt aataaaatta gcaaataaaa cagaagcatg tgtaggagag
    20161 caaatagatt ttgtaatagt agtatcaaat gcaggaccag aaaatgctca aaatgttact
    20221 ttaatagaca atgtttcaga taagcttaag aaagcagtat tttcacttga tagaggtgta
    20281 agtttccaac catggactgg aagtcttaat ataggtacac taccagctgg aactttaaga
    20341 gtgattatat taagaggaat tataaaatca acatgtcttg atagattaac aaatattgca
    20401 gaagttactt caacaacacc agattcaaac cttaacaata atatatcaag agtgcaagta
    20461 gaaataaaac aattttgttg taatgattgt ggttgtaaag attgttgctg taattgttgt
    20521 tgcaatgatt gtggttgtaa aaattgttgc tgtaattgtt gtaaaaatga cttttggtgt
    20581 tgattttata aaataatttt gtatacctta aattaaactg aaataaaaaa taaattcaca
    20641 tatgagtaaa atattttata aatattaata aattttctat ctttgatatg gaccacattc
    20701 gtaatactag ttattaaatg aa
//
LOCUS       DAMJWU010000062        20698 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMJWU010000062 DAMJWU010000000
VERSION     DAMJWU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.062"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            50..1927
                     /locus_tag="PC460_002427"
     CDS             50..1927
                     /locus_tag="PC460_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2601308.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTG
                     AYEGTINYNQDPEYLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTV
                     NASQTRLNGSNRYETSLLIAKEIDKNHDVEKIYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKDSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGAD
                     RHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     APGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPD
                     LFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRV
                     LPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSL
                     K"
     gene            2169..4082
                     /locus_tag="PC460_002428"
     CDS             2169..4082
                     /locus_tag="PC460_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601309.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKVTTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4075..6294
                     /locus_tag="PC460_002429"
     CDS             4075..6294
                     /locus_tag="PC460_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2601310.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKSGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLSLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6403..7233
                     /locus_tag="PC460_002430"
     CDS             6403..7233
                     /locus_tag="PC460_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2601311.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7223..10690
                     /gene="addB"
                     /locus_tag="PC460_002431"
     CDS             7223..10690
                     /gene="addB"
                     /locus_tag="PC460_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF2601312.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIINFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10690..14517
                     /gene="addA"
                     /locus_tag="PC460_002432"
     CDS             10690..14517
                     /gene="addA"
                     /locus_tag="PC460_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF2601313.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKSDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14563..15774
                     /locus_tag="PC460_002433"
     CDS             14563..15774
                     /locus_tag="PC460_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF2601314.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15761..19288
                     /locus_tag="PC460_002434"
     CDS             15761..19288
                     /locus_tag="PC460_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2601315.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEIPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19419..20672
                     /locus_tag="PC460_002435"
     CDS             19419..20672
                     /locus_tag="PC460_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2601316.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT         8985 a         1954 c         3676 g         6083 t
ORIGIN      
        1 tgctatggaa aataacgctg atttaaatat aaacttagag aaaaaaagta tcgttttagg
       61 tagcaaatca aaagttagtg tcaaatttaa agaaaaacca gatgcagata gcattacatt
      121 aaagtataaa tgctatgaca tgccattgga tacaactcta aattacaatc aatcaactgg
      181 ggcatatgaa ggaactatca attataacca agacccagaa tatctaaatg tttgggaact
      241 acaagggata acaataaaca gcaaaaataa tcctaaaact ttaaacagac aagacctaga
      301 aaagctggga ttaaatttaa aagactataa tgtaacacag gaatgtataa ttgaagacat
      361 aacttctaga aaagatgtaa ataaatattt gagaaaaact tcttcaccta ttacagaact
      421 tacaggaagt gatagatatg aaacagcagt taaaataagt aaagagggtt ggaaaaatgg
      481 ttcagataaa gtagttataa taaatgggga tgtaagtata gatggcatta tatcaactcc
      541 actggcaacc acatataatg ctccaatact tttggttgaa aaaaacaatg tgcctaatag
      601 tgtaaaatca gaattaaagc gcctaaaccc taaagatata attataattg gagatgagaa
      661 tgctatttct aaaactactg ctaatcaaat taaatcaact gtaaatgcta gtcaaacacg
      721 tttaaatggt tctaatagat atgagacatc tttattgata gcaaaggaaa tagataaaaa
      781 tcatgatgtg gaaaaaatat acataacaaa tgctaatggc ggagaagtgg atgcacttac
      841 tatagcagca aaagcaggtc aagacaagca accaattata ttaactgata aagatagtat
      901 tacagacaat acatataaat ggttaaagag tgaggattta caaaatgctt attttatagg
      961 tggtcctcaa atgatatcaa caaatgttat aaataaggta aatggaataa ctaaggatag
     1021 tgttactaat aatagagtat acggagcaga tagacacgaa acaaatgcaa acgtaataaa
     1081 aaaattctat acagatgatg agttagaggc tgttttagta gctaaatcag atgtacttgt
     1141 tgatgcttta gcagcaggtc cattggctgc gaacttaaaa tctccaatac ttataacacc
     1201 aaagacgtat gtatctgcat accataaaga taatttagaa gctaaatcag ctaataaagt
     1261 atacaaaata ggaggaggat tgacttctaa ggtaatgagc tctatagcat catcattatc
     1321 taaacacaat acgactccaa cagaaccagg aaatagtggg ggcaagacag ttatgattga
     1381 cccagggcat ggtggttcag cacctggaaa ttcatctgga ggaatgattg aaaaagatta
     1441 caatttaaat acttcacttg caacaactga atatttacgt tcaaagggat tcaatgtaat
     1501 aatgacaaga gacacagata agactttatc tcttggaaat agaactgctc tatctaattc
     1561 attgaaacca gatttattta caagtataca ttataatggc tcaactaata aacaaggtca
     1621 tggtgtagaa gtattttata agcttaaaga taaaaatgga gggactacta aaactgtagc
     1681 taccaatata ttaaatagaa ttttagagaa atttaaactt acaaatagag gtataaaaac
     1741 aagagtactt cctagtgatt ctacaaaaga ttatttatac gttttaagaa gtaatgatat
     1801 gccagctgta cttgtagaat gtgcattttt ggataatgaa aatgatatga gtttaataaa
     1861 ctcatctgca aaagtaaaag aaatgggtac acaaataggt aaaggaatag aagattcatt
     1921 aaaataaatg gaaataaaaa tatttaaaaa cttaatgtaa atttatagtt gtaaatttat
     1981 actaaagtca aaaaatgaaa tataagtgta tcaatagtaa gactgtatgt acatttgcac
     2041 atgtgaatgc aagtatatat acagtctttt tttgtagtta aatgcaataa tttgattgtg
     2101 ttttgtaaaa tacacaatat gatataatta gataatatac acttaattta attgaggaag
     2161 gtgattatat ggaatattta tttgaaaaaa gtagtttaaa agaatatgga aatgaagctt
     2221 tagataaata tagagaatta ttagataaaa ctggtaataa ttcaaggtgt attcttttgt
     2281 tagtaccaaa caataacaca aagatacgat atgaaagggc tctaagatta aattatagtg
     2341 aagaactgaa agtaacaaca tatataagtt ttgtgaagaa agaattagtt aaatactggc
     2401 cactaataat agaaaaatgt gatggaataa gtacaaaggt tgtatctcca acatttatat
     2461 caaatagttt aagtgaatat ttaatagtgc aaaaagttaa ggaaaaaagg atacaagaag
     2521 gatattttga agatattaca ggaaccaata aatccatagc aaatagtata atgactaaca
     2581 taaataagtc tgcatttaat ttaattgatt ttgattttat tggagaaaaa atatattcat
     2641 caaagaaaaa taaagatagt atatatagat tttcatacac tcaaatggat gaaattatat
     2701 catattatat aaattcctta ctatcaaatt caattttaga taatgcatta agcatatatt
     2761 tatacaatca atacctactt aatgatgatt tttatttaaa aaacttattt acagaagtga
     2821 gatatataat tgtggatagc cttgaaagct caagtaatgc agaagttgat tttataacag
     2881 aagcattgga taatacatta caaagttata tatattttga ttatagtagg gattattctg
     2941 tatttaataa tgtggatata gactatataa atgagaaaat aatatcaaaa attaagccta
     3001 aagaagaaaa tattaaaaag aaggagatta gaatagaaga tttatactta ttacctgcaa
     3061 atatagaatt aaatcaatca agtcaattat ataatgaaat gttagattta ataagtgaaa
     3121 aagttatatc tcttatagaa gaagatgtat ctccaagaga tatagcaatt atatctccta
     3181 taaacaatag tatattggag catcaaatta gagattcttt gatagaaaaa aatatagatg
     3241 tttttagtac taagaaggat aaaaaagctg tggattatcc atatgggaat gcattggtag
     3301 tggctacatg tatattctat ggatatttag attttataaa agatgatgaa tacttaagct
     3361 ttttagaaac tcttttagaa ataaatagga taaaagcatt taagattttt aatgaaacaa
     3421 gacatttaag tattgataat atagatgaac atagtcagta tcgtgatatt ttacagtata
     3481 ttgaagaaaa aaagaagagt gatataaaaa tacatgaatt tttaactcaa ttttacatag
     3541 ataaaatgtt aaatttaaaa gaaggtaaaa agaatgttgg gttatgtaaa aagataatag
     3601 ctgaaagtga gtcttttagt gaaagtttaa gtctacttgg gatgaaaaaa gaaaagatat
     3661 ttgtagaagc tttaaaaagt acaataaacg attattactc tgttgtcgat atagaagagt
     3721 tgaaatccaa agacaaaata gtaataacta ctccttattc atatatatca tcaaatatag
     3781 atagaagtat acaaatatgg gtagatatag gaagtaatgc ttggaatatg aaaatagaaa
     3841 aagatataag taatttaatt gtacttagaa aatcttttga agaaaaaaag atttatacaa
     3901 atgaaatgga agaatattat aaaaaatatt atctatacaa tacagtatat aatttgttat
     3961 taaatgcaaa gaaaatctat gcatttaaaa gtgaatatgc gattaatggt tatatccaag
     4021 agagtatatt atatagtata cttttaaaaa tctcacataa aggagataaa aattatgatt
     4081 aatataaatt atagagaaga ccagatacca ataataaatt acgataatgg tactatggca
     4141 gtaccagcag taccaggtgc tggaaaaaca tttataataa caaatttagt agctaaacta
     4201 ttacttgaac agaaacataa gagtggtaaa atattaattc ttacatatat gaatagtgct
     4261 gtaaataatt ttaaaggtag aataagaaag attcttgaag agaataaaat agatgatact
     4321 aattcgtatg aagtaatgac tatacacagt ttggctgtaa aaataataaa ggaaaagcca
     4381 gaagttgtaa tgttaagtga ggattttaac atagctgacg atttgcaaaa aagtattata
     4441 ttaaatgaat gtataagtag atttagattt gaaggtggag aaagagcttt tagatggttt
     4501 ttaaaggagc aaaaagatga acgctggaaa gaaataactc ttgaagcctg ggaaagaggt
     4561 ttttttgaat ttgcaggaaa tgcaataagt gaacttaagt ataagggaat ttctccagat
     4621 aaacttgaag aaatattgtc aagaggacac aaaggaatgc ttaaaattat tttaccaata
     4681 tataaattgt atgatagaaa attaaagcaa aatggattgc tagattatga tgatatatta
     4741 attttagcag aaaaaacttt aagcttagac gaagggctta gaaagaagtt tcaaactaga
     4801 tacaaatata tatttgaaga tgagtgtcag gactcaaatg aaattcaagg taatataata
     4861 aaaattatat ctagcgaaaa taaaaaccta gttagagttg gagatataaa tcagagtata
     4921 acaggaacat tttcatcatc agaccctaaa ttttttaaag aatttataaa tagtgcagat
     4981 ttctgttata ggatggatat gtcaaatcga agttcaaaag atatacttga tttagcaaac
     5041 actcttgtca agtatgttac aaaggaattt agacaagaag aatgtagaca tgctcttgaa
     5101 gatatgcaaa taaaaactgt gccaaatgga atgggatata aagaaaaccc taatccagaa
     5161 cattataata taaatgtaaa gtggtatgag agttggaaaa ttgaaataga gcaaactgct
     5221 agatacgtta agggtataaa aaataaatat ccagataaaa gtataggtat attggttcca
     5281 tttaatgaac aagtgacaca agttgctaaa gaacttagag agaataatct aacctttgaa
     5341 gaattaggac caaattcttt gaataaaagg aaggttataa acaacatagc atatattatt
     5401 gattttatgt tacattgtga tgatgtagaa aagttaatag ttgtacttaa taaagttttt
     5461 atagatactg ataatgaaga agaaataaag tattttttag aagaattaaa aaactattct
     5521 accgaagaaa ttatttataa ttttgaattt agtaagttag ataatgattt aatagaattt
     5581 aaagaagaaa gtgatatata cttaagtttt aaaagaggaa tagatattat aaaacagata
     5641 ttagaatatc caataataag gttagacctc ttagttttgt ttattgggaa aaaacttagt
     5701 ttagaaaaag aagataaagc tgtactggat tatatatctt tttatataaa gtatctagta
     5761 tcagaaaata taaatatgga tttagaagat gtatataatt tattgtttga tgtaaagaac
     5821 agggtgttta atcatataat agatgtggta tatgagataa atggatatga accagagcct
     5881 ggtagtataa cactatgtaa ctatcataaa tcaaagggga tggaatggga ttgtgtgttt
     5941 ttactaggtc ttgttgaata taatttccct gacaatatta atcaaaagtt tcagagtgat
     6001 aaatggtatc taaaagaaaa atataaaaac cctatggcaa ttattaaatc agaagttgaa
     6061 gctatattaa aaggaagtat ttcaacagat tatgctcata aaacgaagat tgattctata
     6121 aatgaaaaga ttagactttt atatgtaggg attactagag caaaggaaat gttagtactt
     6181 tcaggaagtg cttatagaga tgaaagtgat atagggaata agagaaaaga gcaaaaacca
     6241 tgtatatatt taagcagact taatcaacat attattgaaa aaaggtcaaa ttaatatata
     6301 ataagttatt aaaagattgt ttgaaatata tctaaaagag gataaaacaa tatgtacagt
     6361 aagtaactac taaaaaataa agttaagaga gaagtgatat gagtgaacga taaattaaaa
     6421 tatttttctt atagtcaaaa ttcaattaac acatataaat catgtccatt gaaatttaaa
     6481 tataaatata ttgatagaat aaattggaaa aatgatgatg ttggtagtag agagtattat
     6541 gaaactttaa aaacaggaag agattttcat ttgatttgtg aaagatattt tagcaatata
     6601 cctcttggaa tctattttaa tgaggatgat aaaaatagta aaaagttttt aaagtggatg
     6661 gaaaatataa aaaaagttgt tcctattgaa aaaggaaaaa cgtatttgcc agaatatgaa
     6721 gtaaggatga cattaaatgg ggatataata caggcaaagt atgatttagt tgtaattgga
     6781 aataacaata tagaaatatg ggattggaaa accgagtcta aaaagattga ctataaacac
     6841 gtagaaaata gaatacaaac tattgtttat atgtttttag caaaagaagt aatacctaaa
     6901 gttttaaaaa tggacataaa tgttaaagat ataaatatga gatattatca acctgaattt
     6961 gatgacttac caattaccat tttatatgat gaagaaaaac atgaagcaaa tagaaataaa
     7021 attcaaaatt atataagtat gataaaaaac actaattatg aagaacatac atacgaagac
     7081 aatctataca atgatataga aaaggtatat aataaagaag aacgtatgtg ctatagaaat
     7141 aaaaagcatt gcaagtattg tgaatttaat aagttatgta atggcaaaga aatagattat
     7201 agtattttgg aggcggaaat ttatgggact taggtttgta ttaggtagaa gtggaagtgg
     7261 aaaatccacc tatatactag atgagattaa aaaagaagct caaaaaaatg aaacgacatc
     7321 aattatactt ttagttccag aacagtacac atttgaggct gaaaatagag taagtaaact
     7381 atttttagga aaagaaaaag ataagtatct aagagttagg gtacttagct ttaagacact
     7441 aagtaatata gttttttcac aagtaggagg attgacagat gtaaatataa attctagtgg
     7501 aaaagcaatg atggtatata gagctattga agatgtaagt gaagaattaa atgtattttc
     7561 aaaatcaaaa tcgcagtctg gttttgtaag ctcaattaca gatatgatat cagaaatgaa
     7621 acaatacaat ataagccctg aaatgttaga aaatatatct ggagaattgg ataatgaaac
     7681 tttaagttta aaattaaaag atataagtaa aatatataat tcttttgaag ggaaattgca
     7741 tgaaaattat gtagatgctc aagatatgtt aacttcattg gcaagtaaaa ttgaattaag
     7801 cagttattta gatggagctt gtgtttatat agatgaattt acaggtttta caccaaatca
     7861 gtacaatgtc ataaagagta tattaaataa gtctaaaagt gttaatatct ctttgactgt
     7921 agatgatatc aactatattg ggtacagtaa atctgatatg ttttccagaa cgaaatttac
     7981 ttattcaaaa ttgactcaat tgtgcaacga agaaggcata aaaatattgc cacaagtaaa
     8041 tttaaacaca ggtgttataa aaagatttga aaaagttaag gaattacagc acttagaaag
     8101 attttataat gcttatccat ataaaatata ttcaaatcaa actgaaaata taaagataaa
     8161 agagtttaac aatttatatt cagaggtgga agagatagcc agagaaattg tacatttagt
     8221 tagagataag aatgtcagat atagagatat aaccattgct acaagagatt taaatagata
     8281 tgatttttta gtacactcta tatttaatga atataatata ccaaacttta tagataagaa
     8341 gagagaggca aaaagtaatc caatagttat tttaattatt tcagctttag aaatgaagaa
     8401 tagacgatat gggtatgaaa ctatgtttag gtatttaaaa agtggactta taggaattga
     8461 taatgatgat ataaatttac tagaaaatta tgtgttggct aatgggatta aaggtaaaaa
     8521 atggtttgat gaaaaatggg attatagaat aacccaaagt ctttcaggtc aagaaagtga
     8581 atttgaactg gaattaaaag agaaaataaa tgaaataaaa aatagagtat tagagcctat
     8641 agtaatttta caagaaaaat taagaggaaa aaatagagta aaagaaatct gtagatatat
     8701 atatgaattt ttattagata taaatatgcc agaaactata gaaagtttaa taataaattt
     8761 taaggataaa ggggaacttg atgttgcaaa tcaatactca caagtttggg acatagtagt
     8821 ggacatatta gaccaaatgg tggaacttat gggtgatgaa ataatttctt tagaaaaatt
     8881 tataaaatta ataaccttag gttttgatga atatgaatta ggactggttc ctcctagtat
     8941 agaccaggta cttgtaagta gtgtggatag aatgaaaaat ccagacacga aatatttata
     9001 cctagttgga actactgatg gtgtgtttcc gctgattaca aaggatagtg gaatattgag
     9061 tgataatgat agggaaagtc ttggaaacaa aggaatcgaa gttgatattg atagtaagac
     9121 taggagtttt gaagaacagt ttcttgtata taaagctctt acttcgacat ctaagaattt
     9181 aaccataaca tatccaatat cagaccatga aggtaaaacg cttagaccat caataattat
     9241 atcaagactt aagaagatat ttccaaatat agaaaataaa agttatttag tggaagaaaa
     9301 taaaagcact gataaagata tacttaaaaa aataacggtt aaatccccta catttaatga
     9361 acttataaac gtaataaaaa attatgacag tgatggttac aatacagaag agattaatag
     9421 tatttggtta gatatatata ggtactattt aaaagatgaa atctacagtt ctataacaaa
     9481 aaaagttata aaaggtctta gttatactaa tcaagttcat aagattgaag agaaaaaaat
     9541 aaggtcattg tataaaagta attctcttag catatctagg cttgaaaaat atgctgaatg
     9601 tccatttgct tattttatac aatatggtct aaaagcaaaa aagcgtaaag agtattcatt
     9661 tacaccacca gatttaggaa cttttattca taatatttta gataggtttt caaaagaact
     9721 tttacaagat aatttaactt ggagagatat tgatgaaaaa tatatagaat taaagatagg
     9781 tataatagtt gatgaaataa ttttaaagat accaggatat atattaaata gttctgagag
     9841 gtataaatac cttgcatata gattaaagaa tatgttgaca actgctataa ctataattag
     9901 tcagcaaata aaacaaggtt catttgaacc aatagattat gaagttaaat ttggcaacaa
     9961 tggaaaatat ccacctataa aaatggtgtt agaaaatgga caagaagtaa gtttaattgg
    10021 acaaatagat agagttgatg aatttgaaga aggagaaaat aaatatataa gaataataga
    10081 ttataagtca ggcaataagt ccataagtct aacagaaata tattatggtc tacaattaca
    10141 attacttgtg tatttagatg caatccttga aagtgcaaag gatgaagata taaatataaa
    10201 tccagctgca atactatatt gtagaataaa taatccaata gcaaaattta atgaagataa
    10261 agatgatgaa gaaatacaag aagcaatatt aaaagaactt agaatgaagg ggcttgtagt
    10321 aaaagactct cacatagtta aagagatgga taaatcatta atagatggag aaaggaaaaa
    10381 ctctttagta ataccagtag gtcttactaa ggatggaaat gtaggtaaaa gtacttcagc
    10441 aataagctat gaggatttca aactgcttag aaaatatgta aggcatgcta taaaggattt
    10501 atgtgaagaa atgttaagtg gagaaatacg tatagctcca tataaacata aagatggaac
    10561 atcatgtgac ttttgtgatt actctgctat atgtcagttt gattcaacta taaaagataa
    10621 caagtataaa aatttaaata ataagagtaa tgaagagata ataaaaatga tgaaaggaga
    10681 tgttaattag tgagttctcc taaatggaca aaagagcaat tagaagttat agaaagtaga
    10741 gaatgcaatt tattggttgc tgctgctgct ggttcaggaa aaacagccgt tctagttgag
    10801 cgtataattc aaatgataac aagtagagaa aatccaattg atatagataa gttattagtt
    10861 gttacattta ctaatgctgc tgcgtcagaa atgagagaga ggatagggga tgctatagga
    10921 aaagccttag atgaaaaccc agaaaataaa catttacaaa accaacttgt tttattaaat
    10981 aaatctagta ttacaacaat acactcattc tgtttggatg ttataaaatc aaactttcat
    11041 agaataaact tagaccctaa tttcagaata ggagaccaaa ctgaatgtgc tattttgaaa
    11101 caagaagcaa tagaggaagt atttgaagat ttatatgaag aaagagatga ggggttttta
    11161 aatttagttg aaagttatgc agaaagagga ggagataaag aagtacaaga tattatcttg
    11221 ggaatatatt catttgcaat ggcttctcct gaacctaaaa aatggttgat tgactcagca
    11281 gaacgattta atattgatga aaattttgat ttttcacagt ctatttgggc tagagctata
    11341 ttagatactg taaaaataga gattaatggt ctttgtctaa atatggaaag ggcattaaaa
    11401 gaagtggaat caatagaaga attagaaact tttgcagaaa agttatccgt tgaatataaa
    11461 aaaattgctg acatttcaca agcatgtaat aaatcttggg atgaggcata taaaaagatg
    11521 gcaagtatgt cctttgaaaa ttatgtaaaa ggagtaaaaa gaatttcaaa agatgctcct
    11581 tcgtatataa aagaatcaaa agaaaaggca aaaactataa gagataagac taagaagtct
    11641 ttagaaagta tagtaagtgc tacattcaat aaagataatg attctattag agaagaaata
    11701 aaatatcttt ataacatagt taaaccaata tctagtgttg tactgagatt tgaagaggaa
    11761 tatagcaata agaaaagaga aaaaggaata atagatttta acgatataga gcattttgca
    11821 ttaaatatac ttacagatgt agatgaaaaa ggtaatattg taccttctga tatagcagtt
    11881 ggatatagaa acaagtttta tgaaatattt atagatgaat atcaggatag taatctagtg
    11941 caggaagtac tgcttaaagc tgttgcaaat acagaaactc caaatagatt tatggttggt
    12001 gatgtaaaac agagtatata tagatttaga caagcaaagc cagaattatt tttgcaaaaa
    12061 tataataact ataatgataa aaagggaagc tcccatagaa aaataatgct ttacaaaaac
    12121 tttagaagta gagaagaagt tgtagatgct gtaaattata tatttgaaaa tataatgaat
    12181 gagaatatag gagaaattga atatacagaa aaagaaagac taaatttagg tgcaaatttt
    12241 aatgtggata cagacgagaa atctattatt ggtggagcaa cggagataca cctaatacaa
    12301 aaagataata aacttgatga tgatattata aatgataaag acgatagaat taataacaaa
    12361 gaaaatgaaa tagaggaaga agaaaaatta gataatatac aacttgaggc tagaatggtt
    12421 ggaaacatta tcaaagattt aatgaaagtc aatgaagatg gaaaaattca aaaggtctat
    12481 gataagggaa tagatggata tagacctgtg gaatttagag atatagttat tcttttaaga
    12541 gctacatctg catgggctcc agtgtttgca gatgaattga tgaatatgga tataccaacg
    12601 tatgctgatg ttggagtggg gtattttgat acgattgaaa taaaaacaat attgtcatta
    12661 cttcaaataa tagataatcc aatgcaagat atacctctta tatctgtatt aaaatcacct
    12721 atatttggat ttacaccaga ggatttgatt gatattagag ttcagagtaa agataaaatt
    12781 ttttatgagg ttttaaaaag tacagcagag tatgatggat ttacagattc tcaaaatgaa
    12841 aatgagtctg agtttatacc aagtgaagaa tgcataaaca aaagtaaaga ttttttaatt
    12901 aagctaaagg aatttaaaga aaagtcaatg tacatgagta cagatgaatt tatatggtac
    12961 ttgtacacaa ggactggata ctatgcctat gttggagcat tgccaggtgg aagtcaaaga
    13021 caagctaatt taaaagtatt gtttgagaga gcaaaacaat ttgaagaaac cagtcttaaa
    13081 ggaatattca actttgtaaa ttttatagag aaattaaaaa aatctagttc tgatatggga
    13141 agtgcaaaaa cacttggtga gaatgcaaat gttgttagaa taatgagtat tcataaaagt
    13201 aaaggtctag aatttccagt agtcatatgc tctgcaatgg gtaaaaattt caatactcaa
    13261 gattttaaaa agagtatttt atatcatcat aatttagggt atggacctca atttgtagac
    13321 tatgagagaa ggatttcttt tccaagtata gccaaagaag cattaaaaag caaaataaat
    13381 atagaaaatt tatcagaaga gatgagagtt ttatatgttg catttacaag agctaaagaa
    13441 aaattgatta taactggttc tactagaaat atacaagata gtataaagag atggtcaaat
    13501 ggaattgaaa gtttagatac aatatcacaa tacgagatat taaaaggtaa aaatttttta
    13561 gattggataa tgccttgtgt attaagacat agggatttat ctaatctact agaagaagta
    13621 ggattagacg cagtttttaa tgttgaacat aattcaaaat ggtatggaaa gttgtggaat
    13681 aagagtgata ttttagtgga aaagaagagt gatgaagaaa aagaaagtat tgaagaaatt
    13741 cttgaaaaaa tagatgtaga taatcctgat agtgattatt acagtgaaat agaagaaaaa
    13801 ttaaactata tatatccata tgaatttagt acaagaaaac ctgctactat atctgttaca
    13861 gaaataaaaa agatacaaaa taattatgaa gaagagttaa taaatactat atttgaacaa
    13921 aaagtaatat taaaaaagcc tctatttatt caaaatgaag aagaaagaga aaaaataagt
    13981 ggaacagaaa gaggtacaat agtccattta gttatggagg ttttagactt aaaaaatgta
    14041 agtagtgtaa atgatataaa atcacaaata agaggttttg tttcaaaagg tataataaca
    14101 gaaaaacaag ctagtatagt aaatccatat aaaatatata aattttttgc ctctaacata
    14161 ggaaaaagaa tgttgaatgc tgaaattata aatagagaaa aatcaattta tgcacaagta
    14221 aatatgaaag acatatatat ttatgaaaag cttataaaca atgacgataa aaaactttat
    14281 gataatgaaa gtgtcatgtt aagaggtata gttgatgctt attttgaaga agacaatcaa
    14341 atagttttgg tggattataa aacagatttt gtaaatgagg aaaatataaa tcaaataata
    14401 gagaaatata aaaaacagtt ggatttatat gcagatatta ttgaaacttt aactggtaag
    14461 tcagtaaaag agaaatgtat atatttattt ggagttgatg aagctgtttg ttattagttt
    14521 ttatatataa attaaaccta aaagatagag gaaggggaaa aaatgagatt tattcataca
    14581 tcagattggc atctagggaa gtccctagaa ggccattcta gaattgagga acaagcaaag
    14641 ttttgtgagg aatttataaa aatagtagaa tcaaatgaaa tagatatggt tataattgct
    14701 ggagatgtat atgatacttc taatccacca gcacaagcag aaaaactatt ttatcagact
    14761 gtatcaagac ttgccaataa tggtcagagg tgtgtattaa taatttcagg aaatcatgat
    14821 aatccagaga gactctctgc aataacacct ctagcacatg aacaggggat acttatatat
    14881 ggttatccat taagtgctac gatagaagct aagtataaag ggtttgaaat aacatatgca
    14941 actcagggat gtacaaaact gaatataaat ggagaaaata ttgtggttgc tacattacct
    15001 tatccaagtg aaaaaagatt aaatgaagtt ttttcaagtg aagatgagtt tgagaaacaa
    15061 aaaaattatt ctgaaaaggt aggagatata tttagaagtt tagaagaaaa ttttagaagt
    15121 gatactataa atatagccgt atcacatata tttgtaatag gtggagagag tactgaatca
    15181 gaaagaccta tacaattagg tggaagtttt ttagttgaaa gaaaagattt gccagaaaag
    15241 gctcaatata ctgcactagg tcatttacat aaacaacaaa aagcttccga acgtttaaat
    15301 gcatactatt ctggttctcc acttcaatat agcaaggatg aaagagcgta taccaaaggg
    15361 gcatacatag tggatataaa agctggtgaa aaaccaataa ttgaagatgt atattttaat
    15421 aattacaaac ctatagaagt atttaaatgt aatggaatag aagaagcact agatatatgt
    15481 gagcaaaatc aagatagaga gatttggtca tattttgaaa taaatactga tgagataata
    15541 tcacagaatg aaattaaaaa aatgaaggaa ctattaaaag acataataga aataaaacca
    15601 ataataacat cttgttatga acaagaaagt gtagatataa aagaaaagtc tatggcagag
    15661 ttgtttagag agttctattc ttttagtaaa ggtgtagagc caaaaggaga acttatggat
    15721 ttatttctag atataataag tgaagaaggt gaatcagcag atgagaccaa ttagattaga
    15781 acttacaggg cttaatagct atattgataa acaagttata gactttgaaa aattaattga
    15841 aagaggtttg tttgggatat ttggaactac tggaagtgga aaatctacaa tcttagatgc
    15901 aataactatt gctatgtatg gaaatatatc cagaaataca aaagaatata taaacagtgt
    15961 gtgcgataag gcaattatat catacgagtt tgagatagga agcaaaaaca ctagaagaag
    16021 atacattgta gatagaacca ttgctagaag caaaacagga acaaagacct ctaatgctag
    16081 attaatagaa gtattgaatg acaataccca aaatgtttta gctgataaag ttgtggaagt
    16141 aaatgaaaaa gtagcgcagg tcgtaggatt aacggcaaat gattttacta ggtcagtagt
    16201 tttaccacaa gacaaattta atgagtttct aagattatca cgtgcagata gaagagacat
    16261 gttagagaga atttttaatc ttgagaaata tggaagaagt cttggcgaaa aagtgaaaaa
    16321 gagaaaaaat atacagttac aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga
    16381 tggaattaca gaggaagtat ataacaatat aaaccaagaa ttaatagagt taaaaaattt
    16441 ggaaaaagat aagaataatg ctttagacct agctcaaaaa tcttatgaag aaagtaaaac
    16501 agtatatgaa gaacaattaa agttagaaaa aaatgaactt agaaaaaatg agcttgattt
    16561 aaaaaatagt gagataaaag aaaagattgc tcttgtagaa aatgatagta acgctaaaaa
    16621 ggtagaccca tacataagtt cagttcaaaa tctagaaaaa aagatagatg aagatagctt
    16681 cgcagtaagt aatttagaaa agaaattagc aatattaaat caggagcttg aagttacaaa
    16741 aaatagatat gaaaaaataa acaaaattaa gaatgaagaa atccctaaat tgagtgaaga
    16801 aaaaataaga ttgcaacaag caataaaatt agaagaagag ttagttttat tagatagaga
    16861 gttaaaagag ctaaaagaaa gtggaattaa tttaaataag actaaagttg aattagaaaa
    16921 agtaaagcag gtgtcagagt ccagaaaaga tgcagttact aaatctataa aagaagttga
    16981 aggaaagatt gataaagtaa atataagtgc agaattaaaa caaaaaatat ttctagcata
    17041 tgaatacgag aaagattaca acaacgtatt ggaagaaaaa aatcaaaagc taaataaact
    17101 agaagaaata ctcaaagaga cagaaaatat aaatttaaaa gttagatata ttgataaaga
    17161 taagaatgat gtaaatcgta atttagaaaa tttatcactt cacttggatg ttcttcttaa
    17221 aaaatgtcct ggtaagagtg ctgatttact tttaaaatct gaatatgtta ctgaattaag
    17281 aaataaagca aataatacta aagaaaatga aattaagaaa agcagtatac aagatgaatt
    17341 aaagataata cttgaaagta agtttaatac tgaaagggaa gtaaatttac taaatgaaaa
    17401 acttgaaaat aatagaaaga acagagatga cttagaaaaa gaattagaag aactaaagta
    17461 tttaaattta gcctctgaac ttagacgtga attaaaagaa aatatgcctt gtccagtttg
    17521 tgggtcaaaa catcatgaaa atcatgatat aactaagtat gatgaaaata tatcttttgt
    17581 caaagaaaaa ctggagaaat tagaaaaaga aaaaataagt ataagaaaca atatagagga
    17641 attaaatgct aaagtaagtg ggtatttatc tatagaaaaa atgaagacta aagaacttga
    17701 agatgtaaaa ggaaaactag gggggattcc atctagtcaa ttattaaaaa aacttgatga
    17761 agaacaaaga aaacttgcat tgttaaaatc taatattcag gagtgggaga aagaaaaaga
    17821 gagtactgaa aataaagtaa cattagctaa agaagaaaaa aataaaattg aaaaagaaga
    17881 attaaaaata agagagagtc tgaataatta taagaaatta acaagagatt taaacataga
    17941 aatagaaggt ttagaaaata aatgtaaaaa acttaaacag gaatacctag gattaaagac
    18001 gattactaaa gtctctaatc tattgtcaaa agtaaaagaa ataagggaga atgaaaaaga
    18061 attagaatta ctaagtactg ggcattcaaa tttattaaaa aatagagact cactagatat
    18121 taaaataaga gaaaatgaga gtaaactaca tgaaatagag atagaattaa taaaggctag
    18181 agaattatat gtagaaaaga aattgagtag agataataag tataaagaag ttattagcat
    18241 aacgaaaggt gatttagcta aaaatctttt acataatgtt gaagaaaata tatgtaagat
    18301 attagaacaa gaagaatcaa gtaagaaaaa attggaagag caaagattag aatatgaaaa
    18361 aaacattgct gaaaagcaca atatagatgg tagacttaaa actgctaagg aacaatataa
    18421 agaccaaaaa tcaacactaa ataaactatt ggcagaaaat aaatttgaaa gtatatatgc
    18481 agtaaaaaga gctttattag atactgatgc agtaaaaaaa ttgattgaag aaatactaga
    18541 gcatgaagaa gaacaaaagt tattatcatt taaaataaaa tcatcaaaag aaaaattaaa
    18601 tggaagaagc ataaagaagg agtactttga acagttaaaa gatgaaattt ataatttgaa
    18661 agtagaaata ggaaatatat ccaaagatat tggagctaat caaaatcagt taataacatt
    18721 aaaagactct ttagataaga taaatgattt taataaacaa cttaaggtag ttgaacacaa
    18781 tgtggactta ttggaagaat tagataaatg tgtacaagga aataaatttg tagaatatgt
    18841 ttctacaaac caattaaaat atatagcgtt ggaagcatca aaacgattgg atggaataac
    18901 aaaaggaaga tatgcacttg aaattgactc aacactaaac tttgtaatga gagataactt
    18961 taatggagga gaaagaagaa gcattgatac tctatctggt ggagagacat tcttgacatc
    19021 tctatcatta gcacttgctc tttcatctca aatacaatta aagggaagtg caccattaga
    19081 atttttcttt ttagatgaag gttttggttc attagatagt gaacttttag aaattgttat
    19141 agaatccttg gaaagattgc atagtaataa tctaagtgtg ggtataataa gtcatgtcga
    19201 ggaacttaaa aatagagttc ctgttaagct attagtttca tctagtgagg ctggaatagg
    19261 ttcaaaagta aaaattgaat atagttagaa aatatatgtt aattgaatat attatctaat
    19321 atttccaaat aatatgtaat tgtgatataa taaaaactaa tattaaagtt atttataatt
    19381 aatattaata taaaaacatg taagtaggag tgtggtaagt gttggaatcg cccaataatt
    19441 tgattcagat tattttttta atagtattac ttatagggtc tgcatttttc tcagcatctg
    19501 aaacggcctt aatgtcgttg agtaaaatca gaatcagata tatgcaagat gaaggagtta
    19561 aaggagctaa gttagtaagt tcattaatag aaaatccaaa taagctttta agttctatat
    19621 tggttggaaa taacgtcgta aatatagcag caacatccat atcaacatct ttgtttatag
    19681 gattaatggg agaaaaagga gtggcacttg ctacagctgt tatgacagta ttagtattaa
    19741 tatttggtga gattactcca aaaacaatag cagcaaataa ttcagaaaag gtttctctat
    19801 tagtatctaa acctataaaa gcaattatat ttatattaag accaatagta tggatattta
    19861 atataattac aaatattata tttaaattat ttggtataac aaataaagga gctaaatcat
    19921 tcataactga agaagagcta aaaactatgg taaatgtaag tcatgaagaa ggcgtccttg
    19981 aaatggaaga gagagaaatc ataaataatg tttttgaatt tggagatatg caagctaaaa
    20041 atgcaatggt acaaagaata gatatggtag ctatagatat ggaagatagt tatgatgaga
    20101 taatacaggt atttaaaact gaaaaattaa gtagaatgcc tgtgtatgaa gaaactatag
    20161 atgatattgt aggaatactt aatataaaag atattatatt tttatctgat gaagaaatag
    20221 agtcatttga tattaaaaat tatatgagag aacctttttt cacgtatgag ttcaaaaaga
    20281 taacacaact tcttgaagag atgaaacttg aaaaaagtca gatggctata gttgtagatg
    20341 agtatggtgg aacatctgga cttttaacta tagaagattt agtagaagtc attgttggag
    20401 atatagagga tgaatatgat gaagaagagg atgaaataca agttataaaa gaagatgagt
    20461 atattgtaga tggaagtact aaaattggtg atgttaatga acttattggt gtaaacttgg
    20521 aatctgagga gtttgattcc ataggaggtt ttataattgg acacctaagc agattgccag
    20581 aagaaaatga agtaattgaa gttgataata taagattttg tatagaaagt atagaaaaaa
    20641 atagaattaa aaaaataaga atatatacat aatgtaataa tgttcttaaa tttattag
//
LOCUS       DAMJWU010000063        20253 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMJWU010000063 DAMJWU010000000
VERSION     DAMJWU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.063"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            79..3027
                     /locus_tag="PC460_002436"
     CDS             79..3027
                     /locus_tag="PC460_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2601317.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3005..3469
                     /locus_tag="PC460_002437"
     CDS             3005..3469
                     /locus_tag="PC460_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2601318.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3582..3884
                     /locus_tag="PC460_002438"
     CDS             3582..3884
                     /locus_tag="PC460_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2601319.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3946..5310
                     /locus_tag="PC460_002439"
     CDS             3946..5310
                     /locus_tag="PC460_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2601320.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFASVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5345..5818
                     /locus_tag="PC460_002440"
     CDS             5345..5818
                     /locus_tag="PC460_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601321.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            5990..6640
                     /locus_tag="PC460_002441"
     CDS             5990..6640
                     /locus_tag="PC460_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2601322.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6814..7746
                     /locus_tag="PC460_002442"
     CDS             6814..7746
                     /locus_tag="PC460_002442"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2601323.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8020..8733
                     /gene="ispD"
                     /locus_tag="PC460_002443"
     CDS             8020..8733
                     /gene="ispD"
                     /locus_tag="PC460_002443"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF2601324.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8730..9215
                     /locus_tag="PC460_002444"
     CDS             8730..9215
                     /locus_tag="PC460_002444"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF2601325.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9310..9518
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9582..11297
                     /locus_tag="PC460_002445"
     CDS             9582..11297
                     /locus_tag="PC460_002445"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2601326.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11419..12864
                     /locus_tag="PC460_002446"
     CDS             11419..12864
                     /locus_tag="PC460_002446"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2601327.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13304..14785
                     /locus_tag="PC460_002447"
     CDS             13304..14785
                     /locus_tag="PC460_002447"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF2601328.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15006..15161
                     /locus_tag="PC460_002448"
     CDS             15006..15161
                     /locus_tag="PC460_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601329.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15306..16703
                     /locus_tag="PC460_002449"
     CDS             15306..16703
                     /locus_tag="PC460_002449"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF2601330.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16688..17101
                     /locus_tag="PC460_002450"
     CDS             16688..17101
                     /locus_tag="PC460_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF2601331.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17207..17965
                     /locus_tag="PC460_002451"
     CDS             17207..17965
                     /locus_tag="PC460_002451"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF2601332.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            17978..18715
                     /gene="rlmB"
                     /locus_tag="PC460_002452"
     CDS             17978..18715
                     /gene="rlmB"
                     /locus_tag="PC460_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF2601333.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18718..19245
                     /locus_tag="PC460_002453"
     CDS             18718..19245
                     /locus_tag="PC460_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF2601334.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19310..19957
                     /gene="sigH"
                     /locus_tag="PC460_002454"
     CDS             19310..19957
                     /gene="sigH"
                     /locus_tag="PC460_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF2601335.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            20031..>20253
                     /gene="tuf"
                     /locus_tag="PC460_002455"
     CDS             20031..>20253
                     /gene="tuf"
                     /locus_tag="PC460_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2601336.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETP"
BASE COUNT         8391 a         2095 c         3781 g         5986 t
ORIGIN      
        1 aaaaaaataa taagtacaaa aaaaacaata tatggcatat aagttgctat ataaataata
       61 aggagggaac aattataaat gaggcaaaga ataaaaaact taattgaaaa tgaagataag
      121 aaaaatcctt taacagatga agcgatttca agtaggttaa atattcgaag agaagatgtt
      181 acctttttga gaaatgagtt aaaaatagaa gattcaagac aaagaagaaa agttgtttta
      241 attaaagcta taaaagatat tttaaaagaa gaaaaaacta taaataaaaa tgatataaca
      301 agaagattaa acagcattgg gtttacagta tcaagattta cagtcataca atacttaaaa
      361 gagattcaag attctggtga cctaaaactt gataaagaga gtgatgataa aaaagaagaa
      421 ataatcaata atagagaaaa taaagatata gcatttgata aacttatagg tagtagtggt
      481 agcttaagta caattataaa actagccaaa gcagcaattc tttatccacc tcatggtctt
      541 catactatta ttatggggcc aactggtgtt ggaaagagtg agttagcaga gtgtatgtat
      601 aaatttgcag tagaatcatc cagattccct aagaatgcac catttatagt ttttaatgca
      661 gcagattatt cagagaatcc acagcttttg ctatcacagt tatttggaca cgttaaaggt
      721 gcttttacag gagctgatga gaacaaagaa ggattagtat caaaggcaga tggaggaata
      781 ttgtttatag atgaaataca tagattacct catgaaggtc aagaaatact atttcaactg
      841 attgataaag gtatggtcag aaagttagga gaaacaagac taacacataa aatagatgta
      901 atgattattt cagcaacttc tgagccaata gattctcatt tacttaatac atttaaaaga
      961 cgtatacctg taacaattga aataccagaa ttagttgcaa gaccattaaa tgagaggttt
     1021 gacattataa ataatttctt cttaatagaa gctcaaagaa tgggagcaaa catacatata
     1081 aagtcagatg tattaaaagc tcttatgctt tacgattgta taggtaatgt aggtcaactt
     1141 agaagtgata ttcaagtagc ttgtgctagg ggactattaa accaacttac aaacaaacta
     1201 aaagaagtaa atataagcat atcagatgta cctggatatg taaaacaagg tcttataaaa
     1261 atcagaaatt gtagagggaa aatagaaaat tatgtagatg gggatttaat tatagactca
     1321 aatcttcaag gtgagataga aaagaaaaat gaagatatat atacttttcc agatgaaata
     1381 tacaaactta ctgaaagaag acatattgat ttattggaac aaggtttaga ttttgatgtt
     1441 ataaatagaa tcattggagg agaattagaa agttgcattc aaaaatacat aaaacaagtt
     1501 aaaaaagttt ctattagtaa caatcttcca gatataagtc caattgttgg aaaatatatc
     1561 gtagagctaa cttatgaagt aatcaaaata gcatcacaat acctaagttc actagaaccg
     1621 aatctacatt tatgtttagc agttcaccta aaagcagctt ttgagagatt aaaagaagga
     1681 aaagtaatat caaatgctca tttgaaagaa ataataggaa attatccagt agaatattct
     1741 gtagcagaaa aggtagctca atatttaaag gaaagatata acataaactt accaaaagaa
     1801 gaaataggta taatagcaat atattttaaa atgagtagta gaagagatat gtctgaacaa
     1861 aaaaagatag gcgtacttct tatggctcac ggaagagtag catcttctgt atgtgaggtt
     1921 acaaataaac tattaggaat taatcacgca aggtatttag atatgccatt tgagaagaag
     1981 ccggaagaaa tgattgaaga agctactaca ataataaaag atattgatga aggtagaggt
     2041 gtgattatat tagtagatat gggttcactt tcatattttg gagatattat ttctggaaag
     2101 actggaatta atataaggac aatatctaga tttgatacat tgctttctat agaggttata
     2161 cgaagagcag ttttaccaga tttaacaata gacgagatag ttgatgaagt taaaggtata
     2221 gagccacaag taaaagaaaa ggttagtaaa tgtaacaata caaaagtaga ttttagaaaa
     2281 cctataattt taacattatg tattactgga ttagggggag cttgtaagat aaaaaaaatc
     2341 ttagaggaaa atattaaaag tataactgaa aaggttgata tagaacctat aggtatgatt
     2401 gaatatccaa gtcttgattt agagataaaa caaataagca atgataaaaa tgtaatcgcc
     2461 atagttggaa caattgaccc tcagatagca aatatccctt tttttagtct agtagacata
     2521 aatgataaag cgaagttaaa tcaaataaat aacctaattg atacaaatca aattggtgaa
     2581 acaaataaaa aatatgaatt atcagattta atacataaag aagatatatt tgtggattta
     2641 caggttaaat caaaagaaga agttctaaaa tatatgagta aaaaacttag agagaaaggt
     2701 tatgtgacaa aaaatttcta taaaaaagta gtggaaagag ataacttatt tccaactgaa
     2761 ttatcagatg aaatagcaat acctcacaca gatggaatag atgtaattag gccagctata
     2821 agtatagcca tcttaaagaa gcctattgtg tggagtaaaa gtaaagtaaa aataatactt
     2881 ttattagctg tagaccaaaa gtgttttaat ccattaagca ctctactctc atttatagaa
     2941 acagatgaat ttaagaagat aaaaaacatt aaagatagag attttattag ggagatgata
     3001 ttagatggag tcaatgaaaa taattagtag caatttaatt tttaaaaata ttgaagtgtc
     3061 taacaatgaa gatgcattaa agtttttggg acaacgtttg tttgacgaac agtatgtaaa
     3121 agaaagttac atacaagctg ttgtagcaag agaaaaaaag tatgctacag ggttaccaac
     3181 agaaatatac ggagtggcaa taccacatac agatatagta catgtaaatg agccaggaat
     3241 agcaattgga atactgaata aacctgtaaa atttataatg atgggtacag acgatactga
     3301 aattgatgta aaagttgtat ttatgttagc agtgaaagag ccacaagaac aattgcaact
     3361 attagaaaaa cttatgacta tatttcaaga taagaatata ctaaataata ttgttgactt
     3421 aagtgaagaa agtgtgagtg atttattaaa ctctaaattg aaaaattaaa catgtaaagt
     3481 atattaaaaa tgtaaaatta aacatgcaaa gtatgttaaa aaatataaaa agattattaa
     3541 agatttctaa ctatagatat ctttaaaata aaataatttc aatgggggtg actaatgtgg
     3601 ctaaaaagat attagttgca tgtggaacag gagtatgtac ttcaacaatt gctataaaca
     3661 aattaaagaa agcacttcaa gacattggta aattggatat ggttaatatt acacaatgta
     3721 aagtagtaga agttgcatca aaagcacctg attatgattt aataatttgt actacacaag
     3781 tatctagtag tataaaaaca ccagtgataa atggattacc ttttttaact ggtgtcggaa
     3841 tggataaatt aatcaatgat gtattagaag agttagaatt ataattaaaa aaattaagga
     3901 acatggtctc taaaatcata agttttagag acttaaaatt aaataatgga gggtctttat
     3961 atggagatat taaatttcat tgttggtcta ggtgcacaag taatgatgcc aataataata
     4021 tgtatttttg gtttgatatt aggtacaaaa ctaggaaaat ctttaagagc tggtttaact
     4081 gtgggagttg ggtttatagg tcttaacact attatagctt tgctaacaga taatttaggt
     4141 ccagctacac aacaaatggt taagaattta gggctaagtt tatcaataat agatgtagga
     4201 tggccagcag catcagctat agcttttgca tcaacagtag gggctttaat tattccaata
     4261 ggtcttgtgg taaatatagt tatgctaatt actaatacta ctcaaactgt tgatgtagac
     4321 atatgggatt actggcattt tgcttttaca ggagctttag tggcaggtgc tactcaaagt
     4381 gtaatgtggg gagtatttgc atcagtagca aatatggtaa ttgtattggt aatggcagat
     4441 ttaactgctc caggtataga ggaatattta ggtatgccag gtatatctct tcctcatgga
     4501 tttacacagg catttgttcc aattgctata gtagtaaata aattattaga cctaatacca
     4561 ggtataaata agattgagat aaatgctgat acattgcaga aaaaatttgg attatttgga
     4621 gaacctttaa taatggggtc tgtaataggt gtaataattg gtattgctgc gaaatatgat
     4681 ataaaaggaa tattacaatt aggtgtaact atgggagctg tattaatatt aattccaaag
     4741 atggcagcat tattaatgga aggattatta ccagtatcag aagctgcaca ggaatttatt
     4801 gagaagagat ttaaaaacag aggcaagatt tatataggct tagattcagc agtaggaata
     4861 ggtcatcctg ttacattatc agttgcacta gtgttggttc ctttgactat acttatagca
     4921 gctatattgc caggaaataa agttttacca tttgcagatt tagcagtaat tccatttgca
     4981 cttgtattaa tcgtaccaat tacaaaggga aatgtgttta gaacattaat aataggaata
     5041 attataatta cttctggatt attaatcgca actaacttag ctccattatt tacccaaatg
     5101 gctttaaatg ctagttttaa aatgcctgaa ggagcaacta tgatttcaag tatttgtgat
     5161 ggtgcgaatc cattaagttg ggtatttgta aaagttatga actataaagt tattggagga
     5221 gtagtttttg gagttatagc tttaggaatg gctatttaca ataggaatag aataataaat
     5281 gaaaataaaa agctatctat agaagaataa ggttaaaaca attttattaa ggagtgtata
     5341 ggttatgaaa aaatataaat atgattctaa aagatactta ctcagtgtta caataccagg
     5401 attttttatg attttaatat tgatttatgc actgtttaat aactatacaa attttaattt
     5461 gaatatatat agtttgttaa taattgtctg tacttatggt atattcaata cttttatatc
     5521 atcaagtaat cctaaaaaaa ttattattga tagcaaatgt atacatttta cctcatttat
     5581 gactacacat aagtatgaaa ttaaagatat agaaaagata agaataagag aattttataa
     5641 taaaaaaatt tatgtaaaaa ttaatgatgg aaaccttttt aaaggtaggt attggataag
     5701 aacaaatatg tttaataata gtatagcatt atatagctat ttcattgagt tggaagagtg
     5761 cttatatcca gattcactaa aatttagaaa taaaaaattt gagaataaga acatataaaa
     5821 ataaaaaatt gaattgaagc ctagcttaat cttttttgat aagatataag tttgaacttt
     5881 atactaaatt tttagaattt tcaatcaaga caataacata atagctaagc aattagaaca
     5941 caattaagat gtataaggca tgtatatgat gaatgggagg atttaattaa tgttattaga
     6001 gaaattaaga aaagaagtat tacaagcatc tttagacctt ttaaactata atttagtgac
     6061 attaacagga ggaaatgtaa gtgggagaga tgaacaaact ggctatatag caatcacccc
     6121 aagtggaatg gattatagaa atcttacacc aagtgatatt gttatagtag atgtagatgg
     6181 aaatattata gatggaaaat ggaaagcatc agtggattta tcagaccact tgtatatata
     6241 taagcataga gaagacataa atagtataat tcatacacat tctacatact caagttgttt
     6301 tgctatttta aatgaaccta tagaatgtgc aagtacaact cttgcaaatg aagtaggggg
     6361 ttcagtacca gttgcaaaat tttcaccacc aacatctaaa aagatgggaa aatgtgttat
     6421 tgaagcaatt ggtgacaaaa gagcatgtct tttagcaaat catggcgtaa ttgcagttgg
     6481 tccatctgta ggacatgctc ttacagccgc tgtaatgtta gaagattcag caaaagtata
     6541 ttacttagca aaatcaattg gcacaccagt gctactgcct gatgaagaaa ttcaaagagc
     6601 gagagatgta ttttttaatg tctatggtca ggataaataa attagataaa attttaaaag
     6661 taatatgaaa atatagatat tcaatgatat taaggagatt tataatgata taataagttt
     6721 aatagttaag aagtaaaata tgaaatgtaa tagtatataa attcatttaa gactaaaaag
     6781 aataagataa aataaaaagg agtctgtatt tctatgtatt acttattaaa caaaagcaca
     6841 aatccttact ttaatttggc attagaagaa tatctatttt taaatgataa atataatgat
     6901 gatattataa tcatatggag aaatgaagaa tccattttta taggtaaaaa tcaaaatcca
     6961 tatcaggaag tatatcatga tgttattgaa aagggagaaa ttcctatttt aagaagaata
     7021 tctggaggag gaactgttta tcatgactta ggtaatatca atatgtcatt catacaaaaa
     7081 gataggcaac ttcatgagat agattttttg gagcatacta agtttatgca aaagatgctt
     7141 tcaaccttag gactagatgt atctataact gaaagaaaag atttattttt aaatgggaaa
     7201 aaaatttcag gctctgcaca gtctataaaa agaaaaaatt ctctttatca tggaactctt
     7261 ctttacgatt cagatttaaa taaattaact aaatatttaa attcaaataa agcaactgaa
     7321 tcaaatgcaa ctaaatctgt ttcaagtaaa gttacaaata ttaagttttt actcgaaaaa
     7381 gatataaatt actttttaga ttattgtgtt gaatatttaa aatctaatat aaagaatata
     7441 aaagaacttg aattggatga agaagatatt aaaagtgtat acaatatagg agaagataaa
     7501 tataaaaaga tagagtggac atatggtaaa acgccgaaat ttcaagtagt gctaccattt
     7561 aataatgaaa gtaaaataaa tatacatgtt aacagatgga aagtatcaaa attttttatt
     7621 tcttatgatg ataatgtaat taatatggac aagttgttag atttgaattt ctttaaagaa
     7681 gaaataaaag agagtatttt agaaaattat ccacattaca tagatttaat caatttaata
     7741 ttttaatata aaacctctaa tttgataaaa ttgtcttata tcaaactaga ggtttttaat
     7801 attcttgcat attaacaacc attacaaaat tgtaaaatta aaaacaacac tattgaaaaa
     7861 tcaatatata ttgtaataaa tatatgtgtt tttcattagc aaatagacat taactattta
     7921 tacaactgaa aatttaatat ataattaatc agatggttaa aataaaaata ttaaaagttt
     7981 taataaatga tggttaaaaa taaaatacga ggtgatacca tgtatagtgt tataattgtt
     8041 gcagcgggaa gcggaaggag aatgaactta gatataaaca aacagttcat aaaattaaga
     8101 gaaaaagaaa ttattgctca tacaattcaa gttttttatg aaaatataaa tatagatgaa
     8161 atagtggtat gtataaaaaa agaagaagaa gattttttta aagaaaatat aataaataag
     8221 tataacttta aaaatataaa aatagcatat ggcggaaaag aaagacagga ctcaatatat
     8281 aatggtttaa aaaaattaga tgaaaattgt gatatagtac ttatccatga tggagcaaga
     8341 ccatttgtcg accatagaat aatcaatgaa tctataaagg tagctaaaga aaaaaaggct
     8401 gtggttgtag gtgttccagt aagtgatact ataaagatag tgtcagatgg aacagtacaa
     8461 gaaactccag aaagaaattt gctgtgggca gcacaaaccc ctcaaacctt tgaatataat
     8521 ctcataatag atgcttatga acaagcatat aagaacaatt attatggtac tgatgattca
     8581 atgttggtag aaaatatagg tcaaagtgtc actatggtaa tgggttctta tgaaaacata
     8641 aaaataacta gtccagaaga tttaaatata gcagaacaaa tattaaatat ggaaaaaaga
     8701 gatgaagtta acagtagaag gagaattata tgagaatagg attaggatat gatgtacaca
     8761 aattaacaga ggatagaaaa cttataatcg gtggtgtaga gataccacac gataaaggtt
     8821 tgttgggtca ttctgatgct gatgtattga tacatgcaat aatggattct attttaggtg
     8881 ctctagcatt aggagatata gggaagcatt tcccagatac agatgaagaa tataagggag
     8941 cagatagtat gaaattatta gaacatgtgt ataatctaat aacaagtaaa ggatataaaa
     9001 ttggtaatat agacagtact ataattgctc aaagccctaa gatggctcca tatattgaga
     9061 gtatgagaag taatatatca aaagttttaa acacagacat tgataatata aacataaagg
     9121 caacaacaga agaaggtctt ggttttacag gagcaaaaca gggaatagct tcacaaagta
     9181 tttgtttatt attgttgaca agtcagaata attaatatat tattagtaat aagtttaatg
     9241 gatatcaagt aataggccta tatagaatat ctataatgta tgaatttaag tgaaattaat
     9301 ataaatataa aattagctat gataaagaaa tagtaaaatg atgaaatctt cacagagaga
     9361 aaacggtggt gagagttttc aagaggaagt ctttgaacct gtcttttagc ttaaaggtat
     9421 aaaaatcttt acggtaggaa ccgttatatt cccaaaagag agctattcat atagctaact
     9481 agagtggtac cgcggaaatt aaacctttcg tctctatact tgtatagaga tgagaggttt
     9541 tttatatttt caggaagtta atattcagga ggaatctatt aatgaaaatg tcaaaaatgt
     9601 ttatgccgac attaaaagaa atcccagcag atgctgagat tacaagccat caattaatgg
     9661 ttaggtcagg catgataaaa aaaatgactt caggtgttta taaccaatta ccaatggggt
     9721 taagagtgtt caaaaaaatt gaacaaataa ttagagaaga attaaataaa aaagattgtc
     9781 aagaaatact ttgtgcagcc ttacttcctt ctgaattatg gaaagagtct ggaaggtgga
     9841 ctgctatggg tgaagaaatg tttagactaa aagacagaac agaaagagag tactgtcttg
     9901 ggccaacaca tgaagaagca ttcactgata taataagaca agaaattact tcatataaac
     9961 aattgccact aaacttgtac caaatacaag taaaatatag agacgagaga agaccaagat
    10021 ttggtgttat gagaactaag acttttacaa tgaaggatgc atatagtttt gatgtagatg
    10081 ataaaggttt agataagtca tatcaagata tgtttgatgc atatgtaagt atatttgata
    10141 gatgtggatt ggagaatagc cctgtacagg cagattcagg agctatagga ggttctacat
    10201 cagccgaatt tatggttaag tcagaagttg gtgaagatga agttgtattt tgtagtggat
    10261 gtgattatgc agccaatgta gaaagagcag aatcttgtaa tctagcatca caaaaagaag
    10321 aaatgaaaga attagaagag gttcatacac caggagcagc tacaataaaa gagttagaag
    10381 aatttttaaa aacttctcca gataagtttg ctaaaacttt agtatatgag gcagatggaa
    10441 aaacagttgt agtcgttgta agaggagata gagaagttaa tgaaatcaaa gtatctaatg
    10501 ccataggttc tgttatagaa tttgctcttg ctacagatga tgtagtgaga aaagtaacta
    10561 atgcagaagt aggatttgca ggtccaatag gtataaatgc tgactatgta tttatagata
    10621 aagaaattgt tgaacaaaga aacatagttg ttggggctaa taaaactgag taccatataa
    10681 aaaatgctaa ctatggaaga gattttgaag gaatagttgg agattttaga aatgttcaag
    10741 aaggcgacaa gtgtatagta tgtggtaagc cacttgaaat agctagaggt gttgaagtag
    10801 gacatatatt taaactagga actaagtact cagaatctat gaatgcaaac ttcatagaca
    10861 aagatggaaa atcaaagcct atagtaatgg gatgttatgg aataggtgtt gaaagaacag
    10921 cagcagctat aattgaacaa cataatgatg agaaaggtat aatatggcct ttgtcagtag
    10981 ctccatacca tgttgtgata gttccagcaa atatgaaaaa tgaagaacaa atatcaatag
    11041 ctgaaaatat atataatgat ttgcaagcta tgggtgtaga agttttatta gatgacagag
    11101 atgaaagaat aggtgtcaaa ttcaatgatt cagaattaat aggaatacct atgaggatta
    11161 cagtaggtaa aaatattaat gaaggtaaag tagaatttaa acttagacat aaagaagata
    11221 aagagataat tgacatagaa gaaataaatg aaaaagtaaa ggcagagttt atcaggaata
    11281 atgttaggtt aggataataa cttaaataag atattgtata gataatcaaa gttgtacttt
    11341 atagacacca ttttatctat atataaggat ataaaatggt gtttttttat aaaacaaata
    11401 aatgaggagg taattaaaat ggcaaaaaat gaaaaacaat ttgttgaaga aataacaaag
    11461 atggaagatg attttccaca atggtacaca gatgtaataa ctaagactga cttagttgat
    11521 tatgcaccag taaaaggttt tatggttgta aagccatatg gatatgcatt atgggaaaag
    11581 atgcaagaat ttatggataa aaaatttaaa gaaactggac ataaaaactg ttattttcca
    11641 ttattaatac cagagagttt attaaacaaa gaagcagaac acgttgaagg ttttgctcca
    11701 gaagttgcat gggtaacaca tggaggaaat aaaaaattag aagaaagatt atgtgtaaga
    11761 cctacttcag agactataat atgtacaatg tatgcgaaat ggttaaaatc atatagagaa
    11821 ttaccatatc tttataatca atggtgctca gttgttagat gggaaaaatc aactagacct
    11881 ttcttaagaa catctgagtt tttatggcaa gaaggtcata cgttacatga aacagcagaa
    11941 gaagcacaag aagaaacaat acaacaatta gaagtatata aagcgctatg tgaagaatta
    12001 cttgcaatgc cagttgtagc tggtcaaaaa agtgaaagtg aaaaatttgc aggtggtgaa
    12061 agaacatata ctatagaagc tatgatgcat gatggtaaag cattacaatc aggtacaagt
    12121 cacttcctag gtcaacactt tactaaggct tttgatatta cttttgcaga tagagaagga
    12181 aatttagcta atccatatca tacatcttgg ggagcatcta caagacttat aggtggactt
    12241 ataatgactc atagtgataa cagaggttta gtattaccac caagagtagc tccaattcaa
    12301 gttgttatag ttcctatagc tgctaaaaaa ggaaatgtaa tggagactgt tgataaaata
    12361 tatgctgatt taaaagctaa gggagtagct gtagaagttg atgatagaga taactataca
    12421 actggatgga aatttaatga gtgggaaatg aaaggtgttc cagtaagagt tgaaattgga
    12481 ccaaaagata tagaaaataa tcaagctatg gtatttagaa gagatacttt agaaaaggat
    12541 tctatgcctt tagaaggatt agctgatgct atatgtgatt tatttgatgt tatacacaat
    12601 gatatgtttg aaaaagcaag aaaacataga gaagacaata catctatagt tgaaaatatg
    12661 gatgagttta gaaaagcttt agaagaaaag ccaggattca taaagactat gtggtgtgga
    12721 gatgcagaat gtgaagctaa gattaaggaa gaaacaggag caactataag atgcttacct
    12781 tttgaacaag aaaacttagg tcacaagtgt gtgtactgtg gaaaagaagc agatagtatg
    12841 gtagtaatgg caaaagctta ctaaaataca tttaaataaa aactggggta gaaagtattt
    12901 aatactttat cccagttttt ttgtttttta tgagtaataa aatgtatttg taactatgtc
    12961 ataagataag tcatagatac ttatattaaa aagaatataa tagagaacta tttttaataa
    13021 ttacatatta atgaaagcaa aatatttttg ttatatagat tttcaataaa gcaaacaata
    13081 aatataaatg aatattagta aggagtgctt ttaattttta gaaaataaat gacttaaaag
    13141 aaagatttat gaaattaaaa ctaggatttt ctgatataat aaacatgtgt atttttaaat
    13201 aaaaaggata ttattattag atttgattaa aaataaactt gcaaaacaat gtactagttt
    13261 aatattatag ttatattgaa taaaatatca ggaggaagta aaaatgaatg taagagtaag
    13321 gtttgcacca agtccaacag gatttgtaca tataggtagt ttgagaactg ctttatataa
    13381 ctatttattt gcaaaaaaaa tgggcggaga atatatatta agagtggaag atacagacca
    13441 aagtagatta gtagaaggtg ctatagagaa tatgcttaat gctatgaaat gggctggggt
    13501 caaccatagt gaaggtgtta tattagatga tagtggaaaa gtagtacaaa aaggagaata
    13561 tggtccatat attcaatctc aaagattaga tatataccaa gaatatataa agcaactttt
    13621 agatagtgga aaagcttatt actgtttctg tacaaaagaa agactggatg aagtaagaga
    13681 tgctcaaaga gcagcaggag aaacagctaa atatgatggt cattgtaaga atctttctaa
    13741 agaagaagta gaagcgaata taaaagcagg aataccatat gttataagat taagattgcc
    13801 agagaatcat acaattaagt ttacagactt agttagagga gatatggaat ttaatactaa
    13861 tgacttagat gaccaagtat taatgaaaac agatggattt ccaacttatc attttgcagt
    13921 agttgttgat gactatttga tgaaaattac acatgttata agaggagaag aatgggtatc
    13981 atctactcca aaacatgttt atttatatga agcttttgga tgggaagcac ctgtatttgt
    14041 gcatttacca aatatactta ataaagaaaa gaaaaaatta agtaaaagac aaggtgatgt
    14101 agctgttgaa gactttaaga aaaaaggata tttaccagag ggattagtaa actatgtagc
    14161 attagttggt tggtctcctg aagataatca agaattgttt acaatggagg aattagaaaa
    14221 agccttctct gtagaaagag tctctaaaag tggtggagta tttgatactg aaaaattaaa
    14281 ttgggttaat cagcattata taaaagatgg tgatgatgct tatctaacag acttagcaat
    14341 accattttta atagaagatg gatttataac agaagaagaa gctactaata aatatgattt
    14401 cttaaagagt atgatatcag tacttaaaga gaaattacaa tatgtaaaag aagttactga
    14461 acatgcaagt atattttttg gagataaaat agaggtagaa actgaagagg gaagagagtt
    14521 cttaagatta gaacatatac ctactttaat agatgctttg agagaaaaaa tagaaaaaac
    14581 agaggtatta aatgctgagt ttgttcaagc tatgcttaaa gaaatacaaa aagagtacaa
    14641 aataaaaggg aaaaatttat ttatgggctc aagaataatt ttgacagggc aaatgcatgg
    14701 tccagattta ccaaaagtaa tggaagtttt aggaaaagaa acttgcttaa atagaatagc
    14761 ttatgtaaaa aataatatat tatagtcata aaagtccgct aatttagaag cggacttttt
    14821 ttgtgttagt atttggtgaa aatatctgct taagaaaatg aaggtattta aggtaataaa
    14881 tttaatatat aaaaagcaaa gcaaaaatat aaataaaata tgaataataa actaataaaa
    14941 tattaagcat agaagaaggg aatctattat tttagtttga tttaattatt aagggggtaa
    15001 ttactatgta tagtaatatt gatgatgtta aaaaggaatt aaaggagcta tgcttagaat
    15061 atgttacaat tttagaaaaa ttaaaagatg aaaaaatgat tacagaagaa acttttgaaa
    15121 agtgctcttc tcaaaaaaaa atatttttag aagaacaata gaggatggta atagtattat
    15181 tattatgtag tttgaaagat agtacgaaaa atgatagtaa tactattatt tattgcaatt
    15241 ttcaactata atataatata taatgttaag aagataattt ataaaaatac agtgaggtga
    15301 tgagtgtgaa agtttataat acattgacta gaacaaaaga agaatttgtt ccattagaag
    15361 aaggaaaggt aaaaatgtat gtgtgtggtc caacagttta taactatata cacataggta
    15421 atgcaagacc atttataata tttgatactc taagaaggta tttagagtat agaggatatg
    15481 atgtaactta tgtacaaaac tttacagatg ttgatgataa gattataaat agaagtcatg
    15541 aggagggaat atctcctgaa gaagttgcgg ctaagtatat aaaagaatat tttgtagact
    15601 gtgatgggct aggaataaaa agagctactg ttcatcctca ggttacagat aatatacaac
    15661 agattataga gtttataaaa gagttggaag ataagggata tgcatatgca gtaaatggtg
    15721 atgtatattt tgatacaaat aagtttgaag gttatggaaa actttcaggt caaaaacaag
    15781 aagatttaga ggcaggggct agaattgagg ttaatgacca aaaaagacat cctatggact
    15841 ttgttctttg gaaggcaaaa aaagaaggtg agccaggatg ggatagccct tggggagaag
    15901 gtagaccagg atggcatata gaatgttctg ttatgtctaa gagatattta ggtgaaacaa
    15961 ttgatataca tgcaggtggg caagatttaa cattcccaca tcatgaaaat gaaatagctc
    16021 aaagtgaagc tagaagtgga aaaacattct caaaatactg gatgcataat ggatatataa
    16081 atataaatga tgaaaagatg agtaaatcaa aaggtaattt ctttacagta agagatatat
    16141 caaaattata tgatttagag atagtaagat ttttcatgtt atcagcacat tatagaaatc
    16201 cagtaaactt tagtgatgag atgttaaatc aagctaaagc aggtcttgaa agactatata
    16261 atactaaaga aaaattagaa tttacgctaa gtaatttagt agaatctcct ttaacagaaa
    16321 aagaagtaga gcttgttaaa gaattagatg actttagaca aaaattcata gatgctatgg
    16381 atgatgatgt taatacagca gacgcagtaa gtgtaatatt tgaattagct aagttaataa
    16441 attctaatgt agatgaaaat tcttctttgg agtttgctaa aaaatgctta gatgaattta
    16501 atgaacttac aggagtatta aatattgtaa ataagaaaaa agatactgta ttagataaag
    16561 acattgaaga attgatacaa aaaagaactg atgcaaagaa aaataaagag tttcaattag
    16621 ctgatgatat tagacaacaa ttactagata tgggaattgt attagaagac acaagacaag
    16681 gtgttaagtg gaaaaggata taaaaatgga aaaaactgaa ttagttacta tgtcaccatt
    16741 agttttagca tacttaggtg atacagtgta tgagacatat ataagagaat atttaattag
    16801 acagaataca caaagaaaag ttaatgattt gcataaacta gctataaagt atgtaaaagc
    16861 taaagctcag gcaactataa ttcatgaaat tgaaattgag ttaactgaag aagagagtaa
    16921 aatttataaa aggggaagaa atcaaaagtc aaatacttct cctaagaatg cagatattat
    16981 agactacaaa catgcaactg ggtttgaggc attagtggga tatttgtatt taaataatga
    17041 aatagagaga cttcaatata ttatcaataa aggaatcaaa attattgaaa gagatatgta
    17101 aatattaaag agtgttgtct taataataag taataaggca acactttttt tataactaat
    17161 gtataataaa tatatagaaa ttagtaatga gaaaggataa aaagttatga aagtaaaatt
    17221 aatatcacat acacctgaac cagaaaaagt aatagctatg gcagctaagc tatgttattc
    17281 accagttggg acagacgaaa tagaaaaaga cttaacagat gaaagtatag agaaattttt
    17341 aaatatgtta ttaagtatag gtcatgggtc aatattagaa catgcttcat ttacattttc
    17401 aatagaaggt atttcaaggg cttgttcaca tcaaattgtt agacatcgta tagctagttt
    17461 ttcacaacaa agtcaaagat atgttaaatt agaacaattt gagtacataa ttcctccaga
    17521 aatagaaaaa atagaaaagg caaaagagtt atttatagac tccatgaaaa aagaccaaga
    17581 gaattatgat aagttagttg aaatattgtt tgaaaatcat tataatgacc taataaaaaa
    17641 cggtaaaaat gagaaaacag ctaaaagaca agctgaaaag aaagcaatag aagatgctag
    17701 atacgtattt ccaaatgcat gtgaaacaaa aatggtattt actataaatg ctagaagttt
    17761 gtttaatttc tttgaacata gatgctgtga aagagcacag tgggaaataa gaaatttagc
    17821 tgtggagatg ttaagagagg ttaaaaaggt tgctcctata ttatttaaaa agaccggtcc
    17881 aagttgtgta aatggaagtt gtccagaagg aactatgact tgtggagata tagttcaagt
    17941 tagagaaaaa tttaaggcct tgtaggaggt gaataatttg gcgagtatag aaggaagaaa
    18001 tccggtaata gaagcaataa aaagcgacag agaaatagat aaaatattaa ttgcaaattc
    18061 agctaaagaa ggttcaatta agaaaataat aggaatggct aaagataaga atataattat
    18121 tcagtatgtt gataaacata agctagatga agtaagtaca agtcattcac atcaaggtgt
    18181 aatagcttat gcaagtgagt ataagtatta tgaattagat gagctaatag atttagcaaa
    18241 gaataaagat gaagacccat ttttcattat attagatgag ataacagacc cacataattt
    18301 gggttcaata ataaggacag cagatgcagt aggagcacat ggagttatca ttccaaaaag
    18361 aaggtctgta cacataactc ctgtagttgc caaagcatct gctggagcag tagaatatat
    18421 gccagtttgc aaggtaacca atatagttaa tacaataaaa agactcaaag aagaagggct
    18481 gtggattgct gcagctgata tggatggaga aactttttat aaacaaaatc ttactggtcc
    18541 tcttggtgta gtaattggaa gtgaaggttt tggaatatct agattggtta aacaaaactg
    18601 tgactttata gtaaaaatgc caatgatagg taatgttaca tctttaaatg catctgtagc
    18661 aggtggaata cttttatatg agatattcag acaaagactg gataaaagta aataggcatg
    18721 aaaaaaaata taaatcatta cttaataata gatggctaca atattataaa tgcgtgggac
    18781 aatttgaaag aactggctaa agaggactta gaagattctc gagaaaagct tattgatgac
    18841 attatagaat tttctgagtt tatgggttat aagactataa tagtttttga tgcttataat
    18901 gttaaaaatt ctcgagaaaa agttgaaaaa agaaaacaca taacaatagt ttatacaaga
    18961 gaacaccaaa cagcagatag ttatatagag aaatttataa cttctttatc aaagtatgat
    19021 gatgtaaaag tagccactaa tgactatgca gaacagcaaa tgattttagg aaaaggtgct
    19081 actagaatgt ctgcaagaga attaaagtta gagttagatc gttccaagaa taaaatgaag
    19141 gaaaaaaata tcggtttgag gaaaaaaatt cagcgaaatt ggttggagga aaggttagat
    19201 aaagaaacat tgtcgaaact tgagaacatt cgtagaaagc gttgaaatta ctaggttctt
    19261 tagactataa tatacctata aatttgtttt atatatgggg gagatttaca tgttggtagc
    19321 aaaagaaaaa agttatgagt tagtggacaa ttgtcagcag gatgagtata acattgtatt
    19381 aagagcaagt gaaggggata aaatagcact agagtacatt attactaaat acagaaattt
    19441 tgtaaaggca aaagcaaagt catatttttt aattggagca gataaagaag atataataca
    19501 agaaggaatg ataggccttt ataaggcagt tagagatttt gatggaagta agaccaattc
    19561 atttaaatgt tttgcggaga tatgtataac aagacaaata ataactgcta taaaaactgc
    19621 aacaagacaa aaacatatac ctttaaattc atatgtttct ttgaataaac caatatatga
    19681 tgaagagtca gacagaacac ttttagatat tatagctaca agtatagtaa cagatccaga
    19741 agagcttata attagtaaag aagagttaaa aaatatagaa tcaaaaatga acgaattgtt
    19801 aagtgacttg gaattagaag tactagaatt atatttaaat ggaaagtcat accagtttat
    19861 agcggacaag cttaaaagag atgtaaagtc aatagataac gctttacaaa gggttaagag
    19921 aaaattagaa aaacatcttg aaaacagaaa tgattaatag taaaatactt accataagct
    19981 tattgtgagc aaaattaatt tatatcgaaa gataagggag gaaattcaaa atggctaaag
    20041 ctaaatacga aagaacaaaa cctcatgtta atatagggac aataggacac gtagaccacg
    20101 gtaaaactac attaacagca gcaataacaa aaacattata tgacagatat caattaggag
    20161 aagcagtaga tttcgcaaac atagataaag ctccagaaga aagagaaaga ggaatcacaa
    20221 tatcaacagc acacgttgag tatgaaacac caa
//
LOCUS       DAMJWU010000064        20229 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMJWU010000064 DAMJWU010000000
VERSION     DAMJWU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20229
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.064"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..601
                     /locus_tag="PC460_002456"
     CDS             <1..601
                     /locus_tag="PC460_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2601337.1"
                     /translation="VFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQ
                     RELSRKTIGSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKN
                     MIRNRKLSRLISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVK
                     DLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            719..1024
                     /locus_tag="PC460_002457"
                     /pseudo
     CDS             719..1024
                     /locus_tag="PC460_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            1037..1942
                     /gene="truB"
                     /locus_tag="PC460_002458"
     CDS             1037..1942
                     /gene="truB"
                     /locus_tag="PC460_002458"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF2601338.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            2073..3002
                     /locus_tag="PC460_002459"
     CDS             2073..3002
                     /locus_tag="PC460_002459"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF2601339.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3106..3363
                     /gene="rpsO"
                     /locus_tag="PC460_002460"
     CDS             3106..3363
                     /gene="rpsO"
                     /locus_tag="PC460_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF2601340.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3603..4448
                     /locus_tag="PC460_002461"
     CDS             3603..4448
                     /locus_tag="PC460_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2601341.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4573..6684
                     /gene="pnp"
                     /locus_tag="PC460_002462"
     CDS             4573..6684
                     /gene="pnp"
                     /locus_tag="PC460_002462"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF2601342.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            6950..7693
                     /locus_tag="PC460_002463"
     CDS             6950..7693
                     /locus_tag="PC460_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2601343.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7851..9098
                     /locus_tag="PC460_002464"
     CDS             7851..9098
                     /locus_tag="PC460_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2601344.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGI
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9133..9378
                     /locus_tag="PC460_002465"
     CDS             9133..9378
                     /locus_tag="PC460_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2601345.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGDILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9388..10611
                     /gene="dapG"
                     /locus_tag="PC460_002466"
     CDS             9388..10611
                     /gene="dapG"
                     /locus_tag="PC460_002466"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2601346.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10724..11458
                     /locus_tag="PC460_002467"
     CDS             10724..11458
                     /locus_tag="PC460_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF2601347.1"
                     /translation="MKDYREDNEKFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11581..13992
                     /locus_tag="PC460_002468"
     CDS             11581..13992
                     /locus_tag="PC460_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2601348.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13995..15053
                     /gene="mnmH"
                     /locus_tag="PC460_002469"
     CDS             13995..15053
                     /gene="mnmH"
                     /locus_tag="PC460_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF2601349.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            15047..16381
                     /gene="rimO"
                     /locus_tag="PC460_002470"
     CDS             15047..16381
                     /gene="rimO"
                     /locus_tag="PC460_002470"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF2601350.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16368..16910
                     /gene="pgsA"
                     /locus_tag="PC460_002471"
     CDS             16368..16910
                     /gene="pgsA"
                     /locus_tag="PC460_002471"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF2601351.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17198..18244
                     /gene="recA"
                     /locus_tag="PC460_002472"
     CDS             17198..18244
                     /gene="recA"
                     /locus_tag="PC460_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF2601352.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGISVSKEIVEE"
     gene            18420..19961
                     /gene="rny"
                     /locus_tag="PC460_002473"
     CDS             18420..19961
                     /gene="rny"
                     /locus_tag="PC460_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF2601353.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8285 a         2049 c         3906 g         5989 t
ORIGIN      
        1 agtatttgaa aatactaata atcatatagg tttagattta ggaattaaag aattttgtat
       61 ctcaagttgt ggagacttta tagaaaatcc taagtatctt aagaagtcat taaacaagct
      121 tgctaaatta caaagagaat tatcgagaaa aacaatcggt agtttaaata gaaataaagc
      181 aagattaaaa gttgcaggac ttcaagaaca tatagctaat caaagaaatg attttctaca
      241 aaaactatct actaaattaa taaaagagaa tgatattatt tgcatagaag atttacaagt
      301 aaagaatatg attagaaatc gtaaactatc tcgcttaatt tcagatgtat catggtcaga
      361 atttattcgt caattagaat acaaggctaa ttggtatggt agaaaaattg taaaggtagg
      421 taaattcttt gcaagttcac aaatatgcaa taaatgtggg tataaaaacg aggaagttaa
      481 ggatttaaat ataagagaat ggatttgtcc tagttgtaat gaaactcacg atagagatat
      541 aaatgcaagt ataaatatat taaaagaagg attaagacta ataacgattc aaaataaata
      601 acttatgtaa gaaccgtagg aactacgggg atagcctgtg gagaattagt aagacatatt
      661 ttaatatgca aaattctgtg aagcaggaac cctgtgactt tagtcatggg aggttcagtc
      721 aaaggtatct agtatagttt taactcaaga catgatgaat agaaataata ttagctttaa
      781 tgatgttgat ggaataacaa catatacaag agacataaaa ggtatagaag ttggaatttt
      841 atttaaagag aaaaaacaaa atgaaattaa agtgagtttt aggtcaaaaa attatgttga
      901 tgtaagtgaa atagccaagt tatttggtgg tggtggtcat gtaagggctg ctggatgtac
      961 tattagagat agtattgata atgcaaagaa aatggtacta gaggcagtgt taaaatcaat
     1021 ttaaggtgac agtaaaatga ataagattat aagtatattg aaacccacag gtatgacatc
     1081 tcatgatgtt gtaagtagag tgagaaagat tttaaatata aaaaaagttg gtcatacagg
     1141 tactcttgac ccagatgctt caggtgtttt accaatctgt atagggaaag ctacaaaagt
     1201 atgtgaagtt attttaaaca aagataaatc gtatatatgt gagcttactt taggtataag
     1261 tacagatact tatgatgctt caggtgaaat tttaaaaaaa gttgatgatt ttaaatttag
     1321 taatgaagac atagaaagag cttttgacac acaaagagga gagataaatc aacttcctcc
     1381 aatttattca gctttaaagg taaatggaaa gagaatgtgt gatttagtaa gaagtggtag
     1441 acaatctgaa attactttaa aaacaagaag agtaaatata aaagacataa aaatactaag
     1501 tataaaaggt aataaagtaa tgttttatgt tgagtgttct aaagggactt atgtgagaag
     1561 catttgccat gatataggag aatatctagg ttgtggagct catatgtcgt ttttgaatag
     1621 aacatcttct ggtaaatttg acttagataa ttcaataaca ttagaagaat tagaattatt
     1681 ttatgaaaat aaaactttag acaaatactt atatgatata gattatgtcc ttgattcgtt
     1741 taattatgtt gtattaaacc ctaatgctat aaaatattat agtaatggtg gctcaataga
     1801 tgataaaagg tttttaaaaa ataatttcga taaagatgat gaatttgtaa gagtatacag
     1861 tacagataat ttcttaggat taggaaaatt atcaaaacac aataatacaa taagtgttaa
     1921 aagtgataag atgttcatat aaccaatgta tttaaaaaaa caacaaaaga gatagtacta
     1981 taataacttc tatagtctat ctcttttact tatgctataa tagtttgtga ataagttaag
     2041 agtaaattat attttagcgg ggaacattta ttatggtgat tataaaatct ataaaagaaa
     2101 tagcaaacat aaaagaaagt gtcattacca taggaaattt tgatggtgta cacaaaggac
     2161 atcaagttct tataggaaaa acagttgaac atgcaaaaaa agaaaatata cagagtatcg
     2221 tatttacatt tgcaaatcat cctgtaaatt actttagacc aaactcaaca aaaaatatta
     2281 taagcaatga tgataaaata aaattattag aagatttggg aatagatata gttgtaatca
     2341 taccatttga tgagtatatg actaaaatac ctgcaaagga tttcgtagat gagatactag
     2401 taaaaaaatt aaaagctaaa aagatagtaa ttggacatga ctttacattt gcaagggcaa
     2461 aagaaggcaa tgttaatcta ttaaaaagct tagaacatga atttggtttt gaggtagaag
     2521 taataaaacc catcaaaatc aatgatgtaa gagtgagtag tacttatatt agaagtttag
     2581 tatcccaggg tgatatggct aatgtcaagg aatatttggg tagaaattac aagttagaag
     2641 gttgtgttat acattctaaa caccttggaa gaactatagg attcccaacg gcaaatattg
     2701 accttaagaa taatatgctc gttccaaaga gaggtattta tgcttctatt gtacatattg
     2761 gagatgagac ttattttggt gctacaaatg taggctataa cccaacagtt aatggaaaaa
     2821 ctttgtctat tgaaacaaat attttagagt ttgatagaga tatttatggg gaaaatataa
     2881 ttgttgaatt tttggaaaga ataagagatg aaagaaagtt taattcaata gatgacttaa
     2941 aaaatcaatt gtataatgat acaaattatg tgtatgaaaa ctatgtttgc aaaaacaagt
     3001 aatgtatgat aaaatgatta aggaaaataa ccattactca gcatttgaat actccaatca
     3061 atgcttagta ataggcgttt ctaaaaaaat acggaggtaa tctaaatgat aaacaaatca
     3121 gaaataataa aagaatatgg aagagctgaa ggggatactg gttctccaga agtacaaata
     3181 gctttattaa cagctagaat aaacgagtta aatggacatt taaaaactca taagaaagac
     3241 caccattcaa gaagaggact attaaaaatg gttggtagaa gaagaaactt attagcttac
     3301 ttaaaggaaa aagatcttga aggatataga gcattaatag ctaaattagg attaagaaag
     3361 taatttcatt ctttataaaa aagcaggtat agtgtatacc tgctttttta tagttaattc
     3421 attataaagt aaaatagtta ataataaaag tagtttatat gttataaaaa attataaaat
     3481 gaataatatt gggtaagaat attaaaatag tatacaaata cataatcaat ttaaaaggaa
     3541 agacaggata aatatagaaa ttttataaca ggataattag ttggaataag gaggatatta
     3601 ttttgaaaat aaagttagta tgcgatagtt tgtgcgatat tccagatgaa atatcagaaa
     3661 aagattatat agagatagtt ccacttacag ttatttttaa tgatagggaa tatatagaag
     3721 gagtagatat aaagaaagaa gagttttata aaaaagtaaa agaaatcaag caaataccta
     3781 aaacttcaca agctacatat atggagttta aagaagtgtt tgataagttc ataacagaag
     3841 gttatcatat aatctgtatg acaggggctg caaacgcatc aggaactttt caaagtgcta
     3901 tgatagctaa aaatgatgta aatgaaaatg aaaaaataca tatatttgat actagaaatt
     3961 tatctctagg tagtggacaa tatgttataa aggcatgtga acttcttgaa gaaggactgg
     4021 gatttgaaga aattatagat gaattagaaa atactcgcag tagtgtaaga ttgctttttg
     4081 caccatatac acttgatttt ttaaagcaga gtggaagagt tccagttgca actgctttga
     4141 taggaaatat gttaaatata aagcctatat ttttctttga taatggggaa gctaagttgg
     4201 taaataaggt tagaggaata aagaatatag caagtaaatt ggtcgatata attttagaaa
     4261 tgaatgaagg gtctttagaa ggaaaaatcg tcacaatagg ttgtggagat aatttacacg
     4321 attgtgaaat attaaaagat gaagtaaata aaagaataaa agcaagaaga gtattattta
     4381 ctcgaggagg agtttctatt tgctctcata cagggccaga tatattggct ataagttgtt
     4441 ctaattagta gtaatataag gtttttgcat atatatgtga agataatttt atattgttaa
     4501 tttatagtat ttttgataat atagtaaata gagtgatata cgtgtttttt aataagagga
     4561 ggacaagcat taatgtttga acataaaata tttaaaatgg attttgcagg aagagaactt
     4621 tctgtagaaa taggaaagat ttgtgaaatg gctagtggta gttgtatagt tcgatacagt
     4681 gatagtatgg taatggtaaa tacaactaaa tcagccaaac ctagagacgg aatagacttt
     4741 tttcctttaa gtgtggatta tgaagaaaaa ttatactctg taggtaagat acctggaggt
     4801 tttttaaaga gagaaggcaa gccttcagag aaagctatac ttacatcaag attgatagat
     4861 agaccaataa gacctttatt cccaaaagga tttagaaatg atgttcaggt agttgcaaca
     4921 gttctatcag tagaccaaga ttgtacacca gatatagtag caatgatagg ttcttcaata
     4981 gccctttcta tatcagacat accatttaat ggaccaacag gttcagtatg tgttggcctt
     5041 gtagatggtg catttgttgt aaatccaaac gcagagcaaa gagaaaaaag ttcaatgcat
     5101 ctagttgttt caggtacaaa ggaagcaata atgatggttg aagccggagc tgatgaagtt
     5161 ccagacgagg taatgcttga tgctatttta tttgctcacc aagaaataaa aaaaatagtt
     5221 gagtttatag aaggtatagt tgcagaagtt ggaaaagaaa aaatgcctgt tgaattatac
     5281 catgcaggag aagaaataac tcaattggtt cgcgaatttg caactgataa gatgaaaaaa
     5341 gcagttcaga cttttgaaaa gttagagaga atggaaaata tggatagagt taaagaagaa
     5401 actttagctc actttgaaga aactttagaa gattttgaag attttgttgg agatatagaa
     5461 gaagtattac aagatataat aaaagaagaa gtaagaaaac ttatagtaca tgaaaatgta
     5521 agaccagata atagaaaact tgaagaaata agacctatat ggtgtgaaac tggcatgata
     5581 cctagagctc atggttcagc catatttact agaggacaaa ctcaagtact taatgttgct
     5641 actttaggtg ctttaggaga tgttcaaaaa ctagatgggt tagatgaaga agaaaacaag
     5701 agatatatgc atcattataa tttccctgca tatagtgttg gagaagctag accatcaaga
     5761 ggaccaggaa gaagagaaat aggacatggt gctttagcag aaagagcttt attaccagta
     5821 ataccatctc aagaagaatt tccttatgct attagattag tttctgaagt attaagttca
     5881 aatggttcta catctcaagc aagtgtatgt ggctcaacat tatctttact tgatgcaggt
     5941 gtacctataa aagatatggt tgcaggtata gcaatgggac ttataaagca tgatggtaag
     6001 gttgcagtgc tttcagatat acaaggtatg gaagaccatt taggcgatat ggactttaaa
     6061 gtagctggaa cagagtatgg tataacagca atacaaatgg atataaaaat tgatggtata
     6121 gataaagaaa ttctacaacg tgctttaaaa caagctaaag agggtagaat ccatatacta
     6181 ggagagatga gaaaaactat atctcagcca aaaccagaac tttcaccata tgcacctaag
     6241 attgttaaaa tgcaaataaa tcctgacaag ataaaagatg taataggacc tggtggaaag
     6301 ataataacta agataataga cgaaactgga gttaagatag atattgaaca aactggggaa
     6361 gtatttatat caggtattga gattgatatg attaagaaag ctcaagaact tataaataat
     6421 atagtagttg aaccagaagt aggaaaaact tataaaggta aggtaagtag aataatgaac
     6481 tttggagcat ttgttgagat acttccagga aaagaaggat tacttcatat atctcatata
     6541 gctcatgaaa gagttgccaa agttgaagat gtattgaata taggggatga agttgaagtt
     6601 aaagttacag agattgatga aaaaggaaga gttaatttat caagaaaagt tcttttacca
     6661 aaaccagaac ataaaaataa ataacttatt atataattaa aagcaaagtc ttaggatttt
     6721 gcttttttaa atacaaaaaa gtagataatt ttaaaatgat agttatggat ggcttatagt
     6781 tctttttctg tcaggcttta ctcttataaa gaagctattt aaaagctgat taatagagag
     6841 ctttattgat atatatagaa tcacttaagt tatatttagt gcataaacag agcttatttt
     6901 taatagtatt aaataagaaa caatttgtac ataattaggg ggagttttaa tgataatgat
     6961 ggtacttaat aagagtaagc tcaaaaaaat cataatacta attttagtag tgttattagt
     7021 attatcagga ggggtaatgg tattaaagtt atttaacaac aagccagtat ttaatttatt
     7081 taatggtgta gatttaccat atgaaagagg tacaaaggat aaaagtggat atgttgcatt
     7141 aacctgtaat atagaccttg gatgggaaac agagtacgta gaatccattt tagaaacact
     7201 taaaaaagaa aatgtaaaga ttacatttaa tgtaactgga aaatgggcag aaaagaataa
     7261 ggatgaatta ttaaagataa aaaaacaagg acatgaaata ggtaatcatg gatacaaaca
     7321 tttagattat tctacattat catatgaaga caattatgag caaatagaga cctctaaaaa
     7381 gataattgaa gaaattatag gtgaaaaaac taaatttttt caagcaccag caggttcttt
     7441 tggtccagaa acagtaaagg ctgctaaagc attagggtat acttctatca aatgggatgc
     7501 cgatactata gattggaaat acaaagacca accagaggta atcattgata gaatgaagaa
     7561 aaaagacatt aaagatagta gtataatttt aatgcatcca accaacgcaa cgacaaaatg
     7621 tatagatgat attatagcta ttgtaagaga aaaaggacta aaaccaggaa aacttagtga
     7681 tgtatttaag tagacaaagt ataaatattt ggttaagatt gtataagttg tagttgaatt
     7741 aattaaaaga ctgacttata atgtaataca tataaaaaat aaatataatt aaaacatagt
     7801 gttttcaata atccaatatg aaacaaagaa taaattgtta ggagaatgta atgtataaga
     7861 caaaaatact ggaaaatggg cttacaataa ttggtgaaga aataccatat ctaaagtcaa
     7921 taacattagg tatttggata aatgcaggct ctaggataga agaagcacag gtgagtggaa
     7981 tttctcattt tatagagcat atgatgttta aaggaaccaa aaacagaaca tcaaaagaga
     8041 ttgctagttc aatagataat ttgggtggtc aaataaatgc atttacaagt aaagaatgta
     8101 catgttatta tgtaaaacta atagatgagc atatagatac aggaattgat gtattgagtg
     8161 atatgatact aaattctaaa tttgataaaa atgatataga taaggaaaga cttataatat
     8221 tggaagaact taagatgtat gaagactctc cagatgactt atcttatgat ttattagtgg
     8281 aaaatatata tgctaatgat ggattaggaa tgaatataat tgggacaaaa gaatctctgt
     8341 acaatataac tagagaatcc atgttagaat acttaaataa gtattatatt ccaaataacg
     8401 ctgttatttc tatagctgga aactttaact ttgatgatat ggtggagaaa atcaaatcaa
     8461 aatttggaca ctgggaaaag aaaaatttaa gtatagacat aagtgaggct aagtttaatc
     8521 cttgctttat atcaaaaaat aaagatactg aacaggtgaa cttagctatg tgcctaaaag
     8581 gtattccttt tgaaaatgat gaagaagtct attcaatggc agtggtaaat aatattttcg
     8641 gtggaagtat aagctcaagg ctttttcaga aaataaggga agaaaaaggt ttagtatatt
     8701 caatatactc atcacagaca ttatatagga aatgtggtga attaggtata tttgcaagca
     8761 tgagtacaga aaatttacaa gatgtatata atttaataaa gaaagaaatt gaaaatataa
     8821 gagaaaacta tcttacagaa aaagaaatat ctgaaagcaa ggaacagtta aaaggaaatt
     8881 atatattgga tttagaaagt actagcagta gaatgatgtc tactggtaaa tctatgttac
     8941 ttagcaaaaa agtaaaaact acagatgaaa tacttgaatg tataaataat gttaatatta
     9001 attcaataaa gaaggttgtt gataaggtat tcaatatcga aaatattggt acatgtatag
     9061 ttggaagaga tgtagaaaag attctacact tggattaata ttgaaaaaag taaatctctg
     9121 gaggtataaa atatgaattt atctgaaata gcaggtaagg aaatagttaa cttggtcaca
     9181 ggagaaagat tgggggtcat tggagaatgt gaccttatac tagatgaaac tacaggcgat
     9241 atattagcgc ttctaattcc aaaagaaaaa ggattttttg ccagaagaaa agataagtca
     9301 tttttagaag taccatggag aaatgtaaaa aaaataggta atgacatgat aattatagaa
     9361 tatgagggat atatttagga ggaaaaaatg agtattttag tgcaaaagtt tggtgggaca
     9421 tcagtagaat catatgaaaa aatgaatgaa gtatgtaaga tagtaaaagc atacaaaaaa
     9481 aatgatgaag aattacaatt agtattagta gtttcagcaa tgggaagaaa aggagctcca
     9541 tatgctacag atacacttat aaatttgtgt agtgcagtaa atgatgagcc aagtaaaaga
     9601 gaacttgatt taataatgtc ttgtggagaa ataatatcag gaacaattct tgctaattta
     9661 ttgaatgcac aggggataga ttctgttttc tttactggtc agcaagcagg tataattaca
     9721 agtgatgaat attctaatgc aaagataaag tatataaatc ctaaaaaggt aaaaagagct
     9781 ttagatgatg gtaaggttgt agttatagct gggttccagg gtgtaacaga tgatggagaa
     9841 ataactactc ttggaagagg cggaagcgat acatcagcag ttgctattgg taaagcttta
     9901 gaatgtgaaa cagttgaaat ttacactgat gtggatggta taatgactgc agaccctaga
     9961 gtagaaccaa acgctaaagt attgagtttt atagactatg aggaagtatt ccaaatggct
    10021 gacaaaggtg caaaagtaat acatccaaga gcagtacagt tggctaaaag tggaaatata
    10081 actttagcca taaagaatac aatgaatcca acttttgaag gtacaaaaat aggttcttta
    10141 tgtagacact tagaagacaa tatagaatat gaacaagaaa gagattttaa ggttgctgtt
    10201 gcaaataaag atagcgtagc acaagttaag attaagtcag cagaagaagt gtttacagaa
    10261 gtattaaatg agatagaaaa gaaacttata actatagata tgataaattt ctttatatca
    10321 gaaaaagcat ttgttgtaga agatgctgat ataaaatcat taaaggaaat actagataaa
    10381 tttgaattag attatgaagt aaatagagac tgtgctaagg taacattgat atgttcaaga
    10441 attgctgatg aaatgtctgg tatcatgtca aaggtagtta gaggattatc aaaagcaggg
    10501 gtatctcttt tacaaacatc agactcaaat atgactatat cttgtttagt tagtgaagaa
    10561 gatatgcata cagcagtaca tgctatacac caacaatttt atttaaaata aactgtataa
    10621 tacaattatt ttttaatcaa atttaagaat ttcatatata tcttaatata tttgatttat
    10681 ttggtaaaac taaattaaat ataattttga taggagtttt tttatgaaag attatagaga
    10741 agataatgaa aaatttgata aagataattg tagtgaagaa tctagttgcg aagagagaga
    10801 atgtataaag caatttggta ccaatgaaat gccacctcaa cctcctaaag atattcaatg
    10861 tataactata attggagaaa ttgagggaca ttttatagga aatcctcaaa aaaaagctac
    10921 aaagtacgag catataattc caatgctata ctcaattgag gaaagtaatg atgttaaagg
    10981 agtgttagtt gttttaaaca ctgttggagg agatattgaa gcaggtttag ctattgcaga
    11041 acttttaaac agtacatcta aaaaagtagt tactcttgtt ctaggaggaa gtcatagtat
    11101 aggtgttcct cttgctacag caggagatta ctcatttata gcaccaacag caaccatgat
    11161 tatacatcca gtgcgaacta ctggtcttgt gattggtata aatgagactt ttgaatattt
    11221 taaaaagatg caagatagaa taattcaatt tataataaga acatcaaata tcaaaaaaga
    11281 tgtacttgaa aaacttatgc atgaaaaaga tgaacttgta agtgatgttg gtagtgtatt
    11341 aataggaaaa gaagctgtgg attatggtct tatagatgaa gttggaggac taaaagaagc
    11401 acttaaaaaa ttaagagagc ttataaaaga atctgaggaa gaaaaaaata atgattaaaa
    11461 tttgtttaaa taataaaatt tatgatataa tgatgtaata aagtaggagt gttagtgtta
    11521 aggcattagc actttttgct gtgaaggggt aaaacttgct aaaaataagg agtgatatta
    11581 atggccacga aaaagaagaa aaaaaagaag gtaaatacct taagttttaa tgcagaatat
    11641 cataatttaa taacaatatt tataggtgta tttttattat atagtttaaa ttctaattct
    11701 atgggatgga tacctgtgtt aatgcaaaat ctttttaagg gactatttgg agggctttca
    11761 atagcaatac catttatagt aattataaca ggacttttag gattttttga tggaaatgaa
    11821 tatatatata ggctaagaaa gactaaacta tactacataa ttattttatt tatatttgta
    11881 ttttatggat tattaaatgc aggaactctt cctgtagata gcccactaaa aggtaacatg
    11941 tttgatgatg ttatgaaatt gggtgtaagt ggacaaggta gtggtttgat agcaacaact
    12001 attgcgtatt atatgagtaa aatatttggt atagcaggtg gatggttaat aagtatcttc
    12061 gcacttatac tttctgtaat gtttatattt aatatttcta taaaagattt ggtgtcaaat
    12121 gcaaaatcaa aatctaaagc atctaaagac agcaatttaa cgtttaaaga taaaattgca
    12181 aatatgaaaa aatctgcaat agatatgatg actgatgaag tagatgatac tactataaat
    12241 aaaaaaccag gtttctttaa agggttaatg tcaaaaggtc ttaataatga tgatgaagaa
    12301 gatgaatatt ttgaagaaga aaatagtgat ggtgtagatg ataaaactat aaaaatagtt
    12361 ggatttaata aggcagaaga tgagtattta gaaatattag aaggaactca aagtatgcca
    12421 gagttagatg ttttaaaaga attacaaaaa gctaccaatg aaaatccagt tgtagatact
    12481 aaaccagaaa aaaaagttga tatagctaaa cctaatttaa atatagaaaa aactcaacct
    12541 atgagtattg tagcagagcc agttaatgag gattattcaa actataaaaa gcctagcatt
    12601 gagcttttaa ataaagtaaa taagaagtct gatgaaaatg gtaaaaagaa agtgttgaaa
    12661 aatgcttctc ttttagaaaa gacactatca gattttggag tggaagcaaa aattaatcaa
    12721 gttacagttg gacctactat aacaagatat gaaatacaac caagtccagg agtcaaggta
    12781 agtaaaattg taaatttaac tgatgatata gctttaagtt tagcagctaa aagtataaga
    12841 attgaagccc caattcctgg taaaagtgca attggaatag aagttccaaa tgaagaagct
    12901 caaatggttg gtgtgagaga agttctagaa agtgaagaat ttaataattt taactctcct
    12961 cttgcaatgg gacttggaaa agacgtagca ggaaaaatta taattggaga tataggtaag
    13021 atgccacact tactaatagc tggttctaca ggttcaggaa agagtgtgtg tgtaaataca
    13081 ttaataagta gtattttata taaggcaaat cctgatgaag taaaattact attaatagac
    13141 ccaaaagtag ttgaacttgc aaattataat ggaataccac atctattgat accagtagtt
    13201 actgacccta aaaaagctgc aaatgcttta aattgggcag ttacagagat gaatagaagg
    13261 tacaaattat ttgcagatgc tcaagtaaaa gatgtaacaa gttataatga gaaagcggaa
    13321 gaaaaacttc ctaaaatagt aataataata gatgagttag cagatttaat gatggcaagt
    13381 gcaaatgatg tagaagatta tatttgtaga ttagctcaaa tggcaagagc tgctggaatg
    13441 cacttgattg tagcaactca aagaccatct gttgacgtta taacaggggt aataaaggct
    13501 aatataccat ctagaatagc atttgcagta tcatctcaaa ctgattcaag aacaatcctt
    13561 gatatgggtg gtgcagagaa gcttctaggt aaaggtgata tgttattcta tccacttggt
    13621 gcagcaaaac cagttagact tcaaggggca ttcatatcag agagtgagtc ggaaaaagta
    13681 atcgattttg taaaaagtca ggttaaagat ggaataaaat atgaagaaga tataattgaa
    13741 actatatcta aggttaatac ttcaaagggt agtgatgagg atgagttttt gagtgaagcg
    13801 atagaatttg tcgtggaaag tggtcaagca tctgcctcta tgctacagag aagatttaaa
    13861 ataggattca atagagcagc tagacttata gattctatgg aagaaagagg aataattgga
    13921 gcaagtgagg gaagtaaacc gagaaaggta ttaataagca aacaggattt gcagaattta
    13981 gaaggtgaat agaattgtca gttataaaaa tagaaaaagc acttaagcta gataaagtaa
    14041 tatttgtaga tgtgagaact gaaggagaat atgaagaaga ccatatattg aatgcaatca
    14101 atatgcctct ttttaaaaat aatgaacata atgaagttgg aacaatttat aagatgcagg
    14161 gaaaacatga agcaatacaa aaaggatttg actatgtatc gtataaatta aaggatatat
    14221 atttacaagc agcagaactt gcattaaatt atgataatat tgtcatatat tgtgcaagag
    14281 gcggaatgag aagtggcagt atagtaaatt tattatcatc attaggtgta aatgtatatc
    14341 aacttgaagg tggatataag gcttatagaa attttgtttt agagtattta aggcatgtaa
    14401 tggatacaaa aaactttgta tctgttcatg gattgactgg tacaggaaag actgatttgt
    14461 tacatttatt agatgaaaaa ggaatagacg tgctggattt agaaggcata gctaaaaaca
    14521 gtggttctac ctttggtttt ataacttttg ataaaaagcc accatctcaa aagaactttg
    14581 aaacaaaact atttgaaagt atattttttt cgaaagagaa ttatatattt gtcgagagtg
    14641 aaagcaaacg agttggaagt gttacagttc caaatgaaat tttcgaggca atgacacgag
    14701 aaggacatca tatattatta gaatgtgata ttgaaaatag agttgataga ctttgtagag
    14761 attatattta tgataaagat gaaggtaata tattgatttt aaaagaatgt ataaacaagt
    14821 ttagaaaaag attgggtaat aaagtagtag atgattatat tgatttatta gaatcaggtg
    14881 aatataaaga acttgtaaaa agatatcttt tagaatacta cgacccactt tatatgcatt
    14941 cagtagagaa atataagtat agtaagatta taaattttaa tgatacacaa aaagctttag
    15001 aagatgttat agacttatat gagagtatat tggaaggaga aagtgaatgt taaaaatagc
    15061 attggaatca ttaggatgct caaaaaattt agtagatgca gaaataatga tgggaatttt
    15121 aaataacaaa ggctataaat taattggaga ttttgaagaa gctgatgtaa ttatagtaaa
    15181 tacatgtggg tttatagagt cagctaaaca agaatctata gatacgataa taaactttgc
    15241 agagttaaaa aaaactggca atttgaagtt gttgatagta acagggtgtc tagcccaaag
    15301 atattctgaa gaattaaaaa ctgaaattcc tgaaatagat gctatagtag gaacaggaag
    15361 ctatcaaaat atagataaaa tacttaaaga attaagtgaa atgcatcaaa ttgtaagcct
    15421 taatgatata gagtttgtat ttaatgaaga tttaccaaga tatatatcta cacctagcta
    15481 tatggcctat ttaaaaattg gagaaggatg ttccaataat tgtacttatt gtataatacc
    15541 aaaactaaga ggtaaatata gaagtagaaa atttgaagat ataattaaag aagctaagaa
    15601 attagctgaa tctggagtaa aagaacttgt ggttatagca caggatacga caaagtatgg
    15661 ttttgattta tatggaaaag aaagattatc agaactttta gaagaacttg caaaaataga
    15721 tggatttaag tggataaggg ttatgtattc atatccagaa tctataacag aagaattgat
    15781 acaagttata aagaaatatg ataatatatg tagttacttt gatatgccaa tacagcatgc
    15841 aagtaacaat attttaaaat taatgaatag aaaaactact aaagaagata tattaaataa
    15901 aatcaatctg ataagaagta atataccaga tgctatactt agaactacaa taatagtagg
    15961 gtttcctggt gaaacagaag atgacttcaa acaacttgtg gactttgttg aagaggttaa
    16021 atttgataga cttggtgcat ttgcatattc aagagaagaa gatacaccag cagatagatt
    16081 gccaaaccat atagatgaag aagtaaaaat acaaagaaga gatactttaa tgatgattca
    16141 acaaaaaata tctgaagaac ttaatgacaa aaagatagga aaaacttatg aggtgttaat
    16201 agaagaacag atagaagata atgtatatac aggaagaaca caaggtgatg cagaagaaat
    16261 tgacagtata gtgtatgtaa aaagtgtaga caatcttgaa gtaggagaat ttgtaagtgt
    16321 tcaaataaat gatgcaatgg aatacgattt aatgggagat gttttatatg aacttgccta
    16381 acaagttaac tttgtttaga atatttttaa taccagtatt tgttttaatt atgttgctca
    16441 atgtaccaaa taaattttta atagcttgta ttatttttat aatagcttca attactgatg
    16501 ctttagatgg aaaaatagct agaaaataca atctagtcac tgactttgga aaatttatgg
    16561 acccattagc agataagtta ttggttattt cagcacttac atgtatgata gaagaccatc
    16621 ttgtaagtag ttggatggta attataatag tggctagaga gcttacagtt agcatattaa
    16681 gagctatagc tgctgctgat ggaaaagtaa ttgctgctgg aaacagtgga aaacttaaaa
    16741 cgataactca aatggtatct atagtgtttt tattgttagg tgctcagttt gaaaatgtat
    16801 tgatattaaa tattggtgaa attctcattc ttatagctac actacttaca ttatattctg
    16861 gatgggaata tctatataaa aataaagagc tttttatgtc ttctaagtaa aaaattatgc
    16921 tttaataaat aaaaactaat gattaataaa taaaaaataa caacttaaaa ttttaaaaat
    16981 gtaaaaaagg gtatactatt atagtatacc tcttttcatt ttaagaatat tttcttttgt
    17041 gtatataatt attaataatg gtttataaat aaaatgtaat gaataaaaat ataatcatta
    17101 gtagcgtttt taaattgact aggtttgatt tatgttatat aattaatata taacaagaga
    17161 acaaatgttt gtagataaat gaagggatgg tatagagatg agtgtagatc aagaaaaatt
    17221 aaaagcgttg aatgaagctt taggtaaaat tgaaaaagat tttggtaaag gttcagtaat
    17281 gaaattggga gaagcaacat ctatgtctat agatgttata tcaacaggag cgattggttt
    17341 agacatagct attggtatag gaggtctacc tagagggaga atagttgaag tatatggtcc
    17401 agaatcttct ggtaaaacta ctgttgcgct tagttgtgta gcatcagctc aaaaagatgg
    17461 aggaatagct gcatttatag atgcagaaca tgcacttgac ccagtatatg caaaagcttt
    17521 gggtgtggat gttgataacc taataatatc tcaaccagat acaggtgaac aggctttaga
    17581 gatagcagag gcattgataa gaagtggagc gatagatata atagtaatag actcagtagc
    17641 agcattagtt ccaaaggctg aaatagatgg agatatgggt gattctcatg taggtttaca
    17701 agctagactt atgtcacaag cacttagaaa gttaactggt tcaattaaaa aatcaaattg
    17761 tgttgctata tttataaacc agttaagaga gaaagtagga ataatgtttg gaaacccgga
    17821 aactactact ggaggacgtg cactaaaatt ctattcatca gttagattgg atgttagaaa
    17881 aatagataca ataaaacaag gtgataaagt tataggtagt agaactagag ttaaagttgt
    17941 taaaaacaaa gtagcaccac catttaagca agctgaattt gatataatgt atggagaagg
    18001 aatttcaaaa attggagacc ttttagatat agctgctgat gtagatatag ttaaaaaatc
    18061 aggttcatgg tatagttaca atgatactaa acttggacaa ggaagagaaa atgttaaaaa
    18121 attcttggaa gataatttag atttaactac tgaaatagat gagaaagtta gagcatttta
    18181 caatttaaat gaagaacatg aagaatcagg tatttcagta tcaaaagaaa ttgtagaaga
    18241 ataagtaaaa atacaaaaaa ataactagta attaaagaca tgccacgctc cctattagct
    18301 tgacacaatg ttgaaaaaat tataaaatta agtgaggata aaatatctaa atttaatttt
    18361 aattattagg aatatttttt aatcatagtg atgacatttt gggattatta acgaaggagg
    18421 tggatgtcat agatagtgta gtaatgattg taataggtgc agctgttggt attatagctg
    18481 gatattttgt tagaaaaaat atatctgaag caaaaattgg acaagctgaa aacttggcca
    18541 aagaaataat agataaagca caccacgact cagaaacagt acaaaaagaa aaattactag
    18601 aagctaaaga agagatccat aagtggagaa cagaagctga aagagaaaac agagaaagaa
    18661 gaacagaagt acaaaagtat gaaaaaagag tagtacaaaa agaagaagtc ttagatagaa
    18721 aattacaaaa tttagaatct aaagaagtaa acctaagtga aaaattaaag gtagtggaaa
    18781 agaaagaaga agaagttgaa gtaataaaaa ctcaacagtt agaaaaatta gaaagtatat
    18841 caggaattac ttctgataag gccaaagaaa tcatcttaac caatgcggag agagatgtaa
    18901 gacgtgaaat gtctataatg ataaaagaga tagaaagtca agctaaagaa gaagctgata
    18961 agaaatcaag agaaattata ggatatgcaa ttcaaaaatg tgcagcagat cacgtagccg
    19021 aaactactgt aacagtagta agcttgccta atgatgaaat gaaaggtaga ataataggta
    19081 gggaaggtag aaatataagg actttagaaa ccttaacagg tatagacctt ataatagatg
    19141 acactccaga agccgtaatt ttatcaggat ttgatccaat aagaagagaa attgcaagaa
    19201 ttgctctaga aaaattaata gcagatggaa gaatacatcc agctagaatt gaagaaatgg
    19261 ttgaaaaggc gagaaaagaa gttgacaata ttataaaaga atatggtgaa caagctgcat
    19321 ttgaaactag tgtacatggg attcatccgg aattaataag attattaggt agacttaact
    19381 atagaactag ttatggacaa aatgtactta aacattctat agaagttgct catatagctg
    19441 gtataatggc tgctgaaata ggagcagata ttagattggc taagagggca ggtttattac
    19501 atgatatagg taaggctgta gaccatgaaa tggaaggtac acatgttgaa ataggtatgg
    19561 atttacttag acgttataaa gagtctaaag aagttataca tgccatgagt actcatcatg
    19621 gagattacga gcctcaaaca gtagaggctg tattagttac agcagcagat gcaatatcag
    19681 ctgctagacc tggtgcgaga agagaaactt tagaagcata tataaagaga cttgaaaaat
    19741 tagaagaaat agctaattca tatgaaggtg tagaaaaatc atttgctata caagctggta
    19801 gagaaataag aataatggta aaaccagaag ctataaatga tgaagaaata catctattgg
    19861 ctagagatat gactaagaaa atagaggatg aacttgaata tcctggacaa ataaaagtaa
    19921 gcataataag agaaacaaga gctatagagt atgcgaagta aaagaaataa tatctaaata
    19981 ataagaaaat atttttagaa atttaaattt gccacaagat atgataatat atcttgtggc
    20041 ttttaaattg tttaagttta attttatata atgcaaaaat aatctaagtg ctaaaagtgc
    20101 tacttacaaa agattaataa aataaagctt tatatatgta ttatttatat aattatccaa
    20161 gtttgtttga tataatgaag ttgtaaaatc aatactttac tacaaaataa atagtaaata
    20221 ctacagtaa
//
LOCUS       DAMJWU010000065        19336 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMJWU010000065 DAMJWU010000000
VERSION     DAMJWU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.066"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(<1..975)
                     /locus_tag="PC460_002474"
     CDS             complement(<1..975)
                     /locus_tag="PC460_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2601354.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDN"
     gene            complement(1023..1883)
                     /locus_tag="PC460_002475"
     CDS             complement(1023..1883)
                     /locus_tag="PC460_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2601355.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2152..2361
                     /locus_tag="PC460_002476"
     CDS             2152..2361
                     /locus_tag="PC460_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2601356.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2361..2627
                     /locus_tag="PC460_002477"
     CDS             2361..2627
                     /locus_tag="PC460_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2601357.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2656..3228
                     /locus_tag="PC460_002478"
     CDS             2656..3228
                     /locus_tag="PC460_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2601358.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3463..4653)
                     /locus_tag="PC460_002479"
     CDS             complement(3463..4653)
                     /locus_tag="PC460_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2601359.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4913..5344)
                     /gene="dut"
                     /locus_tag="PC460_002480"
     CDS             complement(4913..5344)
                     /gene="dut"
                     /locus_tag="PC460_002480"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF2601360.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5457..7685
                     /locus_tag="PC460_002481"
     CDS             5457..7685
                     /locus_tag="PC460_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF2601361.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7796..8281)
                     /locus_tag="PC460_002482"
     CDS             complement(7796..8281)
                     /locus_tag="PC460_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2601362.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8533..9756
                     /locus_tag="PC460_002483"
     CDS             8533..9756
                     /locus_tag="PC460_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2601363.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9760..12894
                     /locus_tag="PC460_002484"
     CDS             9760..12894
                     /locus_tag="PC460_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF2601364.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12928..14226
                     /locus_tag="PC460_002485"
     CDS             12928..14226
                     /locus_tag="PC460_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF2601365.1"
                     /translation="MSKIVAISCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14515..14760)
                     /locus_tag="PC460_002486"
     CDS             complement(14515..14760)
                     /locus_tag="PC460_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2601366.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(14974..17601)
                     /locus_tag="PC460_002487"
     CDS             complement(14974..17601)
                     /locus_tag="PC460_002487"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2601367.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17662..18504)
                     /locus_tag="PC460_002488"
     CDS             complement(17662..18504)
                     /locus_tag="PC460_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF2601368.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18511..19149)
                     /locus_tag="PC460_002489"
     CDS             complement(18511..19149)
                     /locus_tag="PC460_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601369.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
BASE COUNT         6743 a         3082 c         2676 g         6835 t
ORIGIN      
        1 attatctaag tcttcatcaa acatttttat tatatttttt atctcatact tgtactcatc
       61 ttcattcatt ctataaagca ttaaatcttc tagtacatct ttcatatcga agtacacatc
      121 ttctaataaa taaaaatagc cttcctctat atctttcaat ttatctatag tatccatttt
      181 tttacttata tctttcataa tttctaaatt ttgtatattt acatctatga ttgttaataa
      241 gtacattctc acagttttta ttatttcaac agaaaaatct acaatattat aatttccttc
      301 ttttttatat aatgactcct taaatttgtt aaaacaatta tctaaattag aatttatatc
      361 gttaaatggt ggtcttatta tgtcacgtat tattaaaaga ttttgtatat ctacattatt
      421 tgtgattgcc tcatccaaaa taaataaatt atatgcatca tttaatgggt catgggcatc
      481 tccactaaac tctaagttaa caaattcaag taaatttttc aatgatatat agttcataca
      541 tctcatacca caatttatat atttttcttt tatatctaca aataaatctc taatgttatt
      601 tacaaacctt ggatggttat tttttttagc acttctcata tctgtgttat tgaaacatgt
      661 caaatccaaa ttaccaaaag tatatattcc tttaatatca gaaaatatgc caagccattt
      721 tttaaacttt ctcatgacct cttcataact tggtgcactc ttcaattctt cagaagatat
      781 atgtgttaat tcttgtatat gaggatacaa ctcctcatta cttactggtc ttattaaaga
      841 tttaaattta tcaatatttt ttgttttttc atcatacata actgctccga ttgcaattat
      901 atcagaatta aatactgctc tttttgattt agaatttggc atattcattt caaaatctat
      961 atatactctt ttcaatataa acaccctttc caattgttta tagtgattct ttttaactat
     1021 ttttattcaa aattttctaa gaaagaatgt ttctccccat ctttttgcca accaagtact
     1081 gaatcaagat ttacattttc tgccgcctta aatatagatt tttctacatt tttaaaattt
     1141 gattctaagc tctttattaa gtcttttatc tcatatcttt tattacctgt ttttttagca
     1201 gcaaccatac atgcacaatt taatggccat ataccactat tttgaataaa tcttataata
     1261 tgttcttctc ttatataata aagtggtctt ataattttta taccttcaaa attagtagaa
     1321 ttaagtttag gaagcattgt tttaaaattt ccagcacaca ataaattaag cattgttgtc
     1381 tctattacat cgtcataatg atgacctaat gcaagtttgt tacaccctaa ttcttctgcc
     1441 tttgaatata atgctcctct cctcattctt gcacacatat agcatggata atcttttgct
     1501 atttcatctg ctatctcaaa tatccttgaa tcaaataaat gtattggtat atttagatac
     1561 tcacaattat ctataagtaa ttgtcttata tttgcatgat atccagggtc catagcaata
     1621 aattcaacat caaaattaac ttgtccatgt ttttttaact cttgaaacat tttagccata
     1681 agaatactgt cttttccacc agaaatcgct acagctatct tatcaccttc ttcaaccaat
     1741 ttataatctc ttattgcctt cataaactta gaccataaat ttttcctata tttttttatt
     1801 atactttttt ctatatctct tactggaagt ctttcctcaa aaggaactat aatttcacag
     1861 ccttttcctg atagttcact catctaatca cctcgtatat ttatgttaat aatattttac
     1921 aaaaaagtct accttcatcc acaaaatact ataattttta ctttaatttt actaaataat
     1981 ttatagttta caataacaca taaacttata ataacataaa taacctcttt cttttattat
     2041 cttttttatt tttctcattt aatattttat tattagtaac gatttttaaa tgattgaata
     2101 ttatacacta gatagagtta cctatctatt tacaacaagg aggttttcat cttggaaaat
     2161 aagactagag atatcaaaaa aaattgtggt ccatctttgg ctagaccata cattaataat
     2221 caaattttca cagaaatgta ctgcctttct gatgcattaa aatttggaac tatatttcca
     2281 gaactaaact tactagagtc tgaaatgtac aacaaagaat tatatgctaa gccaaaaaaa
     2341 tatagaggag gtagaagata atgaaagact catctagagc tgaacttatg agaaaagttc
     2401 aggaaacatc ttttgcatgt gttgatatga acttatattt agacaatcac cctgatgata
     2461 aaaatgctat caacacttat aattcattat gtaatcaatt tgcgcaagct agatatgctt
     2521 acgaaaataa atacggtcct ttaactaact ttgggtatgc tccaagtaga tgcccttggc
     2581 aatgggctga ccaaccttgg ccttgggaca gagaatttaa ttattagaca tcaagtattg
     2641 gaggattgaa aattcatgtg gatatatcag aaaactatac aacacccagt taatataaaa
     2701 acttgtgacc ctagaatggc taaatttctt ataactcaat ttggtgggcc aaatggagaa
     2761 cttgctgcat ctttaagata tttaagccaa agatatacaa tgcctactgg taatatgcgt
     2821 gcacttttaa cagatattgg tacagaagaa ctagctcacg ttgagcttat atgtactatg
     2881 gtttatcagt taacttctga tgcaagccca gaagagttaa aagctgcagg tcttggttca
     2941 aactatgctc aaaatggata tggaatttat ccaacagatt caaatggtgt tccatttgat
     3001 gtaagaccta tagcagttat gtcaaatccc gtaaccgatt tacatgagga tatggcagct
     3061 gaacaaaaag cacttgcaac ttattatcaa cttataaacc taacagatga cgttgatgtt
     3121 atagatgtat taaaattctt gggtcaaaga gaaataattc attatcaaag atttggtgaa
     3181 gccttaatgg atgcttacga gttagaagaa tctcaaaaaa tgttctaaaa tttttatttg
     3241 tataactatt attatttgat aatttataat ttactgttgt gttaaaattt ataaaaaata
     3301 taaatataat ttattttcta taaacagaga aaataaataa aaagcaggta tcaatcaaat
     3361 agtaacatgt attatactat acattactat caatattgta cctgctttca tattataagc
     3421 aataaagaaa aatctattta ttaggtttta taataaaaac aattataaaa ctcttcttgc
     3481 atttacatat ctacttgaat agtagctact atttatatta gatattgtta ctttttttga
     3541 tgaacctgaa gatgcatgta tcatatctcc atttcctatg tatattccaa catgacttac
     3601 agaaccatta tttgaacctt gtgtatcaaa gaatactaaa tctccacttc taaggtctcc
     3661 tctatttaca tatgttccat acttactttg gtctctagat actcttggta tactaactcc
     3721 tacagatttt ttcataacgt actgcgtgaa tccagaacag tcaaaactat ttggaccttc
     3781 tgctccccat acatatggtt ttccaagtag tgtctttgca aaacttatta cactatcagc
     3841 tccttgactt ggagaattag tcgaataaga tggaattgaa gtatcaacag aacctttttc
     3901 agcaaggtat tgacttgaca catatccagt ttgattgcca taacttattt ttgtccatcc
     3961 tccactttca gaaatacttc caacctcagt accataactt aatgtagcta ttttagaatg
     4021 acttgtacct ggtcctgttc taacgttcaa tgactttgct gttacaacct ttgtaccctt
     4081 tacaaggtca tttgaactgt tttcagcatt ttcatttgta gagtcattta cactagttcc
     4141 taaatattta cttgaaacat aacctactct accattagaa cttatttttg accatccatt
     4201 tgactcagat atgtatccga cttcagtccc atatcctagt gttgatattt tagaactact
     4261 tgtacttggt cctgttctaa aatttaatcc ttttgctgtt acaacttttg tcgacttaac
     4321 tgaagtatca ctactttcat tgctacttcc aaggctattt attgctacat aatttccatg
     4381 cacataacct gtgtttccat tatatttaac ttttacccaa gaaccttctt ttcctacgta
     4441 ctcaacttta tcacctttgt aaagtttccc cattgattca tggtttgtac ctggtccttt
     4501 tctaaaattt accgcattcc ctgttattgt tctgctttct atactcacac tctctacttc
     4561 agactcatta gcatatgcta aattccccat agtaggcatt attatcgcag ttgccattat
     4621 actggctgct attctctttt gattaaaatt cataactttc cactcatctc ctcttttcta
     4681 ctatctttat ttcacttcaa aatataaaag aaataattga cactatcaaa atatcaatta
     4741 tttcttttat attaatatta ttacaaattt gtaactattt gtctatagta atatttgctt
     4801 accataaatt accatgtata aattttaatc tttaatagcc tgtaatttag taatatagcc
     4861 atcttttacc ttttttaaaa atggctatat tttttaactt ttttgtaact ttttaaaatc
     4921 cagttgagcc aaacccacct tttcctcttt cggagtcact taattctttt acttcttcta
     4981 tgttaatatc atagataggt tttactatca tttgagcaat tttcatatgc ttttcaacct
     5041 taaaatcatt tttaccatga tttattaata taattttaag ctctcctctg tatccttcgt
     5101 caatagttcc tggtgtattt aatacagtaa cagaatgctt taaggcaagc ccacttctag
     5161 gtcttacctg cgcttctgtc atagtaggaa gctctataca tatcccagtt ttgataagtt
     5221 tagtctctcc aggaggtatt acaacctctt ctatagaata caaatccata ccagcatcac
     5281 ctttgtgagc aaaattaggt attatagcat catcatttaa tttttttact tttaaattgt
     5341 acatcttata tctcctttca actttctata aatattataa cttaaatttc gacctcttga
     5401 agttttttat ttttttgtgg taatataatt attagtgttt taaaggagga ttttttatga
     5461 aacttgtact tgctgagaaa ccatctgtgg caaaaactat agcatcattt ttaggtgcaa
     5521 agaccagaca agatggttac tttgaaggaa atgattatat aattacttat gcagttggac
     5581 acttagtatc attatatgat atgaaggatt atgataaaga taaatactct ggctcttgga
     5641 aaatgaacaa tttcccattt gttcctgagg ataagtttaa atttaagatt gatagttcta
     5701 aaactaaaca attcaacact gtaaaaaagc ttttgaatag aaaagatgta gaatatgtaa
     5761 taaatgccac tgataatgac cgagaaggag aacttatagc atttttaata tttttactgg
     5821 caaaaaataa aaaaccagta aaaagaatct tggtaaatga atggacacca gaagatatta
     5881 caagaggtat aaaaaaccta aaagatgaag atgaaatgag aaatcttcaa gctgctggat
     5941 atactaggct tattactgac tggttgattg gaataaattt cacttctgtt gctacattga
     6001 aatacggaaa tgggaaacta ttaaacatag gtagggtaat tcttccaaca gttaaattag
     6061 tctatgatag agatatggaa atactaaact ttgttcctaa aacttattat gaaatagaag
     6121 ggcattttaa agctgaagct ggtgaatata aaggtaaata tgttaagggt aaagaaagta
     6181 aatttgatac acttgaagat gcaaataaaa ttatagcttc aattacatct gaaactggta
     6241 aaatactaga taaaaaagtt actatgtcaa aggaatatgc accaaaactt tttagtttga
     6301 catcacttca aggatatata acttctaagt attccaattt tacatctgat aaggttttaa
     6361 atgtctgtca atctctctat gagggtaatg gtaagggtgg atacataact taccctagaa
     6421 ctgattccat tttcttagaa gaaagtttgg cttcaaaagc ttctcaaact ttagataaat
     6481 taaaggttgg tcttgaatat gaaaataaaa ttaaattttc taaaactaaa agagtttttg
     6541 attcttctaa agtagactcc catagtgcta taattccaac ttatataatt ccaaaaagtt
     6601 tatctaagga tgaacaatta gtttatgatg ccataaaaga tagatttgta gcaaacttta
     6661 tgcctcctgc tgaatatgag aatacagaaa ttaagactga agtagataat tgtactttct
     6721 taacaaaagg taaggtgctt aaatcaaaag gataccttga ggtatataat aaggaagaaa
     6781 aaaacgattt acttccactt gtaaataaag atgatgttgt agatgtgtta gaaataaaac
     6841 cattaactaa gcaaactact ccacctaaac cgtatactga ggacacactt ttaaaggcta
     6901 tgaaaaattg tggtaaaaat gtgcctgaag aagatacaac agtgctatct ggatattcga
     6961 ttggaacatc agccacacgt gctgatgtgt taaagaagat aagtcaagtg ggctatgtaa
     7021 agaaaaaagg taaatcttat tctataacag accttgggaa aaatttagtt gaaatttttc
     7081 ctgttaaaga cttatttgat gtagattata ctggaaaact tgaaaaaagt ttaagcgata
     7141 ttcaaaaagg aaagtataca agaaaagaat atcttactaa tatcattagt tttatatatc
     7201 aaaatgtcaa tattataaaa caagatgctt caaaaaatat aaacacagaa gcatatactt
     7261 tcgattcaaa aactaaaaaa tttactaaaa caaaagaaaa aaaacagaaa aaagatgtaa
     7321 ctaatactga aaaagaagtt gctatatcaa aggattctaa taacaaaaaa gaagttaaaa
     7381 atggtgatga aaatcaatct cttggcaaat gccccatttg tcaaggtgat gtactagaat
     7441 ttgacaaagg ctttgcttgt aaaaatcata aagaatgtaa atttgttatt tggaaaaatg
     7501 ataagtattt aagtctttat aaaaagaaag ttaacaaaac aatggtcaaa aatattttaa
     7561 aaaaaggtga aactaaagta aaatctttaa ctgctaaaaa cggtaataaa tttgatgcta
     7621 cattaaaata tgataaaaat ccagatactg gctatttcaa ttggaaaata gagtttgaca
     7681 actaaatatt cataagaaaa gaaatagcag tagtataata atctatatta tattactgct
     7741 atttcttttc ttatgaagtt tcaacttttt taatagtgac ttttacaatc acactctaac
     7801 ctaatttctt tactatttct gataacttta atgtatgttc atctaataaa gttagttcct
     7861 cttcagaaag attagataat ttttttacta gagattcttt gagaatagct tttactgcat
     7921 caaataattt aaatgcttta tctgttaatt caaccctaag aatccttcta tcttttgggt
     7981 caggaaattt attgactaaa ccatgttcca ctaatctgtc aataatcgga gtcatatttg
     8041 attttaaaat tcctaaatta gaagctattt gagaaattga tgatgaattt gatgtcataa
     8101 gataaagaat taccttagca tgtgatggag ccattggaca ttcttcaaaa taagctttca
     8161 ttatatcaga gttattttga aactttttta acaaatcgct ttctttaaat atattatttt
     8221 caagcacaaa taaaaaatct aaaaaagagt ccgctatttt aagtatattg ttgttatcca
     8281 tatttgtaac atcccttcaa tagataatat aataaaacat tgttgtttaa tatagtataa
     8341 attataaact attaataaat ttaaaacaag aaaaagttat tgactcataa taattataaa
     8401 tttataatca aattagagaa ttataaattg ccaataaagg gaagtttttt aattagtcta
     8461 aaactaaact taaattataa gacactttaa attctacaac aagctagtgt gcaatatata
     8521 ataggaggag atatgaaaag atatttaata gttttcttaa tgtgtagttc actgttttta
     8581 gttggatgtg gaaaaacaga agaatctgaa cctgagaaac caatagcagt ttctgttcaa
     8641 aaagcaatag gtggagaaat tgaaaacaca aattctttct caggaactac aaaggttaaa
     8701 gatgaaacag ctgttactgc acagactgtt ggcacagttc aagaagtgta tgtaaaactt
     8761 gggcaaaatg taagaaaagg agatgaatta ttgagtataa gcagtccaga gctagaaaat
     8821 agtgtaaagc aaagtaaagc ctcactagat ttagcaaaag caagttattc aagtgccact
     8881 ggtggttcat tagaggcaca agtaaatcag gcaaaaacag ctcttgacaa tgcaaagatt
     8941 caatatgagg agtctcaaag aaattatgac aataataaga ttctttatga acaggaagtc
     9001 attagtttag accaatttaa aaaaatagaa ttttctttag agcaaacaaa acaacagtta
     9061 gattctgctc aaagagcata cgatacagca acttcaaaaa gtatacctca agctaaagca
     9121 cttgctaaaa aacagcttga ccaagctcaa gtatcatata atttagcaat gagtaattta
     9181 gacaaattaa cattaacatc tccagtagat ggtacaataa cagctaaaaa ctttgattca
     9241 aaggaaatga taacacaatc acaaccagct tttattattt ctaatcctaa tatactagaa
     9301 gttgatttaa atgtagcaga atcagatata ggtaaattta agaaagatgg aaatgtagat
     9361 gttataattg aagaccaaag gattttagga aaaatagatt atgtaccttc agtagttgac
     9421 cctcagacat ctttatatcc tgttaaagta cttgtaaata atacaaacaa caaatttaag
     9481 gcaggtatgt ctgctcaagt aaacttaagt gttgaaaaag aaaatggtgc tgttacagta
     9541 cctaaaaaag caatatttga agaaaatggt aaaaaatatg tatacatagc aacaaaagac
     9601 aatatagcaa agaaacactt ggttcaaact ggaattgtaa cagaggataa aatagaaatt
     9661 aaaagtggtg taagtgataa agatacagtg attatcggag ggataagtct tatttctgac
     9721 ggaactaaga tatttcctgt agaaaaggag aagtagacta tgaatttaac acagacttcc
     9781 gttaaacgtc ctctcacaat cataatggtc tttttagtat taatagtttt tggtggtatt
     9841 ggatataaaa aaatgtcaat aaatctaatg cctgatatag aaataccagt tgtaatggtc
     9901 atgactactt ggacaggtgc tggtcctcaa gacgttgatg aacaagttag tcaaaaagtt
     9961 gatgaaagtt tatctgccgt ttcaaatgta aaatctacta tatcaagttc acaagaaagt
    10021 gtttctatgg tagttgctca gtttgaattt ggaactaatt tggatgaaat aatgaatgat
    10081 gttaggtcta aagttgatgc attacagacc agcctcccag atgatgcagc taaaccaaca
    10141 gttttaaagc tggatatgaa tgcacaggct ataggtcaac ttgtcatatc tggaggaaat
    10201 gaaaatagtt cacaagcctt aagaaaatac gctgaagatg taattcagcc taaaatagaa
    10261 tccatagatg gtgttacttc agcagacctt aaaggtggag aaaaagcaca ggttaatgta
    10321 atagctgacc cagctgttct ttcaaattat ggagttagtt tatccactat aaaaggtgtc
    10381 ttatcctcta gtaataaaac attcccttat ggttcaatta ctcaaggtga ggataaaata
    10441 gttttaagag cgattgataa attagaatcc ctagatgata taaaacagat tcaaattcct
    10501 gtaaaaggag gaaatacagt tagattagat aatctttgta gtgtagatta tggatatgca
    10561 gacaaagata gtatttatag atacaatgga aaagacagtt tggtaataga tatttctaaa
    10621 caacaagatg ccaacactgt aaaagttatg cagagtgcat acaaatacgt agatgaattg
    10681 aataaggaaa atcctcaatt taaaattaaa attgctaatg atacaagttc atacataagt
    10741 gaatcaataa acagtgtaat gagcaacttg cttataagtg ctgttatcgc atttattgta
    10801 atatttgctt tcctaaaaag tgtaagagct tcactagttg ttgcagttgc aataccaatt
    10861 tctataatag gtgctatagc aattctctac tttactggtg aaagtttaaa tcttattact
    10921 gcaagttcat tggtaatttc agttggtatg gttgttgata atgctacagt tgtaattgaa
    10981 aatatattta aatataggaa aagtggtaga ttgagtcttg atgattgtgc tattgaggga
    11041 acaagaactg taacaaatgc aatacttgca tcaacattga ccacaatagc aatattctta
    11101 cctatcatgt ttacagaagg tatagctaaa ataacttttg gggcattagg taaaacacta
    11161 atagcttcac tagcattctc ctttataggt gcaattacac tcgttcctag tgtatttgca
    11221 aaattaagca gaggaaaaaa tagtcaaaag atgcaagaaa aagatagtcc tatatttgat
    11281 aaggtaagtg aagcatataa aaaattatta agtgtatgtt tgagacatca acgcttagtt
    11341 gtaatatcaa gtttacttat ttttgtagct tccttatttg gaatgggatt tataggtatg
    11401 gactttatgc ctgcagcaga caagggtgaa ttatctattt ctatagaact tccaaaaggt
    11461 cttagcctag aatccaatga ttactatgta tctatgactg agaaaaaagt ctcagatatt
    11521 ccagaaatta aaactttaat aacaactttg acatcagatt caaataatag taaagcaagt
    11581 atagcaattg aattagaatc tcaaaaaaat agaaaaagaa ctactgatga aatagaaaaa
    11641 gagataattg atagagtggc tactgtacca gattgtaaaa taaatgtaag tcaaaacagt
    11701 agtgttatgg gtggagctgg aggagcagac ttcacattag aaatgaaagg tcctgattta
    11761 gatgtattaa aaatactggg aaaagaagct gaaaaagaat ttagctctat aaaaggattt
    11821 agaaatgttg aaacttctct tacagataca acacaagaag ctcaatttac tatagataaa
    11881 cataaggctc aagaatatgg tgtaaatacc gctgaaattg caggcatgtt acgtacagca
    11941 ataaatggtg actccgtaac cactgctact attgatgatt acaaagtgga tgtaaaccta
    12001 aaatttaaag cagatactat tgatagctta gaagatataa agcaactaaa agtaaactct
    12061 gcaactggaa aagaagttcc tcttggtgcc ttttcagata taaaaatggc agatggtcta
    12121 caatctatat ctaggactga tggagatttt tctgtaaata taactgctac acttgacaat
    12181 ttagatgctg gtactgctac aaaactagca ctagaaaaaa cgaataaaat ggtaattcct
    12241 aaagattact ctataggtgt tggtggtgaa acagaaatga tgaatgaatc tatggcaagt
    12301 cttggatttt caatggtcat agctatagca ttagtttata tggtaatggt agctcagttt
    12361 gaatcattta gtaaaccatt tataatcatg ttctgtgttc catttgcatt cgttggagtt
    12421 gtgctttcgc ttctagttag tggtcaaagt ttaagtactg tcggtatgct aggtataatc
    12481 ttacttatcg gtattgttgt taataatggt atagttctta ttgactacat agagcaactt
    12541 agaaaaacaa atcgtaacaa tgatttaatt ggtttggttt caattggttg tgcagcaaga
    12601 ttaagaccag tcttaatgac tacagcaaca acaattttag gtatgttgcc aacagcacta
    12661 tctcttggtg aaggtggaga aactatgcaa ccactagcca tagttataat gggtggtcta
    12721 actgtatcta cacttataac tttggtgctt atacctacta tatacttaat atttgataaa
    12781 tgggaaaata agttttcaga caaaatgcct aaaatatttg gtaagataac agataaaata
    12841 ggaagtataa aacgtaaaat aataaagaaa ccaaaattga aagctaaagt ttaaatagaa
    12901 tggagggtga ttgtattgaa taaaaaaatg agcaaaatag ttgcaattag ctgtggtgtt
    12961 ggtataataa ctgccaattt aatcccagtt tacgcagata ataatacttc ttctataaac
    13021 aaagttgata attctatttt aagtgactca aataaaagaa catctgaaaa ggaaattttg
    13081 actgctagag aagctgttag tgctgcactt aaaaatagtg aaaaattaaa aatgaaatca
    13141 gaagaaataa aaatgttaaa agaaaagtta gaagtacaag atgagtttga tagttttact
    13201 ggaagtgaca actcgtttcc atacgaccaa attggacttc ttaaaaatca aagtgaacag
    13261 gctaaaggct ttatggagga ccaaatagca aatgatatta ctaataaatt taacgattta
    13321 gtttcaagag aaaatgagct agataaaata aaaaacaatc ttgaaataaa aactaaagaa
    13381 attaaagata tgaaattaaa aaaagattta gggttagtaa catctttaga aactgaatca
    13441 gctgaacttg aacttcaaac actacaaaat actcaaaaag ctaaacttca agaactaaaa
    13501 aacaatcaag actattttaa gttattaact aatatagatt taaataatta tcaattggat
    13561 aaagaatata gatttgaatc ttttagagta ggtggctcag ttgattcata tatggaaggt
    13621 aaggttaatg aatatttaaa atacgaccaa ttgattttag aacgtacaga agaaagcttt
    13681 aatgataaag atgagaacaa agcagattta ccagatagac ctaaccttac aagaccagtt
    13741 gctcctacaa aaccagagca aggtaaccta actgatgatg aatacaaggt tcttatggat
    13801 aagtatgaaa aagattatga tagatataaa aaagaagttg aagcatacaa tcttgaaaga
    13861 cagacttatg ctgctggcct tactacttat gcaaattatc ttcaacaaaa atttaatact
    13921 gaaaatggtc tagtaacatt agaagacagt aaaaaagctc ttaaaaaagg gttaattgat
    13981 agctatgctc aattacttgc tttagaagat acaattcaga ttactaaaaa acaacttgat
    14041 ttatcagaaa aacaattaaa aaatactaaa ttaagatatg atttaggatt aattactcta
    14101 actgattata aaaaacaagt ggtaagtaat gaagatgcta aaaatagcta tgacactctt
    14161 attgtgaatt ataactcact aaaaaatggt atagaaaaac cttggatact taatactgga
    14221 aaataattct ggtaaataga aagattactt taacatacgt ttgattaaaa aatagttatg
    14281 catactcgtg gctttagtca tggaagattc agaaggcaaa taatagaaat agattagtaa
    14341 aaatatattt acttataaaa ctgtatactt ttatagaggc tatctcaaaa tagagataag
    14401 cctctttttt attgtttaaa ttataaaaat atatttgatt ccatacaaaa agagtgcctt
    14461 ctgaactaaa aagttcattt tgagacaccc tcaaaagctt aactctaaat ttaattattt
    14521 tttatcaaat gtttcatcta ttttacatct caccatgtta acaaattcag aaatattata
    14581 tcctctaagt ttgcatccac atttaggaca ataatccagt ttcctagata ccaaagccct
    14641 acaactaggt tctggacatc ttatttttag actctcatct gctactttaa atccacatcg
    14701 tgggcatata ctacctggac gtgatgatac ttcctttcca catttaggac actttgtcaa
    14761 ctttatcccc tccagtaaac tcaatttaat tgatagttga aatttctctc tacaataata
    14821 tatgcaatat aattaaatac gtttcactta caactatata agttaaattt ttataataaa
    14881 aatcccctat attcaattta caaatactta atttgataca ttgaacaata ggggatttta
    14941 tttttattta gttacatatt ttgcagtcta ttattatcta ggatttttta tagatgcttg
    15001 agctgctgca agtctagcaa ttggcactct aaatggtgaa caagatacat agttaagtcc
    15061 tacactataa cagaactcta ctgaagatgg ttctcctcca tgctctccac atattcctag
    15121 ttttaattct ggtttaactt ctctacctaa tttagctccc atcttaacta acttaccaac
    15181 accattttgg tctagtactt ggaatgggtc tttttctaat atttcttcat ctacatattg
    15241 acctaagaat ttaccagcat catctcttga atatccaaat gccatttgag ttaagtcatt
    15301 agtaccaaaa ctgaagaagt ctgcttcttg tgctatttca tcagcagtta aacaagctct
    15361 aggtatttct atcatagtac ctacagtata aggaacttct ataccttctt cttttattat
    15421 agcgtcaaca gtttctacta tcatttttct tattattttt aactcattta cttctccaac
    15481 taatggaacc attatctctg gagaaacttt aacacctgct ttgattgctt ctatagcacc
    15541 ttgtattata gctttagatt gcattacaca tatttcagga taagttattg caagtctaca
    15601 acctctgtgt ccaagcattg ggttaaactc atctaaatca actattctct tcttaatatc
    15661 acttactttt attcccatag attttgaaag ttcttctata gtctcatcat catgaggcaa
    15721 aaattcatgt agtggtgggt ctagtaatct tatattaact ggttttccat ccataacttt
    15781 aaataactct acaaaatcct ctctttgcat tggtaatatt ttttcaagag ctttttctct
    15841 ttgttctact gtattagata atatcatttc tctaactgct ggcaatctag cttcatcaaa
    15901 gaacatatgc tcagttctac atagacctat accttctgca ccaaactcta tagctgcacg
    15961 agcatctcta ggattatctg cattagttct aaccatcata catttgtgtt tgtctaccca
    16021 gttcattaat ttttcaaaat ttccagttaa agatacttct gttttcttaa catctcctag
    16081 gtacacattt cctgtagaac catctataga aatgtattct ccttctttta taactacatc
    16141 atcaagtgct cttatttctt tattaaactc atcaactttt atttctccac atccagcaac
    16201 acaacattta cccatacctc tagcaacaac tgctgcatgt gacgtcattc cacctctagc
    16261 tgtaaggatt ccttctgctt taaccattcc ttctatatct tctggagatg tctctagtct
    16321 aactaaaact actttttctc ctttttcatt tgcttttact acatcatcag cattgaaata
    16381 aactttacca cttgctgcac ctggtgatgc tggtaaacct tttgctataa ccttggcttc
    16441 ttttaatgcc ttatcttcaa attttggatg taataattgg tctaattgat taggctcaat
    16501 tctcatgata gcctcttttt catcaattat tccagcttca actaaatcaa ccgctacatt
    16561 tattgctgct gctgctgttc tcttaccatt tctagtttgt agtatatata atctttcatt
    16621 ttctatagta aactctatat cttgcatatc tttataatga ccttcaagta tatgagtaat
    16681 ttttacaaat tcatcgaata cagctggcat tacatctgca agagttgata tattttgagg
    16741 agttcttata cctgcaacaa cgtcttcacc ttgtgcattc attaaaaatt caccaaataa
    16801 tttattttct cctgtagctg gatttctagt aaatgcaaca cctgtaccac tagtttctcc
    16861 catattacca aataccattg attgaatatt tactgctgtt cctaagttat gagctatatc
    16921 attcaattta cggtatacga ttgcacgagg attattccat gatctaaata cagcctctat
    16981 tgcaagcatt aattgctctt ttgggtcttg tgggaaatca ttttttattt cttttttata
    17041 aattgcttta aattctttta ctatttcttt taaatcatca gttgtaagtt ctgtatcaac
    17101 tgtgtatccc tttgcttctt ttactctgtc taatacattt tcaaatttat attttggaac
    17161 ttccatagca acatcagaaa acatttgtat gaatcttctg taactatcat atgcaaatct
    17221 ttcattttgt gtagcctctg ctaagccaac aacactattg tcattaagac ctaggttaag
    17281 aattgtatcc atcattcctg gcatagagaa tactgcacca gaacgaactg atactaataa
    17341 tggattctta ttgcatccta atgttttatt taagtctttt tctaaagtag ctaaatgctc
    17401 ttctatttct tttattatct ctgctcttat actttcattg ttaacataat aatcattaca
    17461 tgcttctgtt gttatagtga atccaggagg gacaggtaag cctatcttag tcatttctgc
    17521 taaattggca cctttacctc ctagtaaaga tttcatgtct ttgcttcctt caccaaaact
    17581 ataaacatac ttagtttcca ttgttaaatc ccccattaat ctttttattt taatttatat
    17641 catttttatt aatagacatt attaaatttc tatttctttg tatatcttta gaccattttc
    17701 tttcattatg tctaaaatta tacctgctgt ttcttctatt gccttatttg aaacgtttat
    17761 tactgggcat ccaattcttt tcattatctc ttctgaataa tctaactcct gaagtattct
    17821 ttctaaatta gcgtagtttg ctttactgga caatcctaaa gcttttaatc tttgggttct
    17881 tatctcattc agcttttcag gtgaattagt aagacctatt atttttttag tatctatctc
    17941 aaacacttct ttaggaattg gtatttctgg aactaatgga acattagcta cttttatatt
    18001 tttattagct aaatacatac tcaaaggagt tttagatgtt cttgatattc ccactaaaat
    18061 tatgtctgct tgaagaacac ctctagggtc ttttccatca tcatatttca ctgcaaattc
    18121 tatagcttca actctcttaa agtaagattc atctaatttt ctaattatac cagcttctct
    18181 tttaggtttt cttcctgttc tcttgcttat ttctcttagt atatctgtca ttaaatctat
    18241 acaactaaga ccttctcttt cgcaatatct ttcaacaata ctgcataatt cttcatcaac
    18301 taatgtatat acaattaaag cgttttcagc ttttgcattc tccaaagttt caagtagtac
    18361 tcctttatta atcatgtatg gaaatctttt tatttcataa gtttcattca cataaaactg
    18421 agacatacat gcctttgcaa cttgttgtgc agtctcccct actgaatctg atactgcata
    18481 tataattagg tttttcacca gcatacctcc ttaattgtca actatttcta agaatagctt
    18541 agttatgttg gtttttgata ttctcccaac aactctcata tgatttttgt cttctccctt
    18601 atattcaaca atcggaatcg aatctacttc atgttctatt atttttcttg aagctaataa
    18661 aacacttgct cctttagtta aagtgactac atttggagtt cttgtcatta tcatacctat
    18721 tggcatttta tttatatcag aacctcctat agttgctttt agcaagtctt ttctggaaac
    18781 aactccacat aactcttcat tctcatctat aataactata ctccctacat cagataaaaa
    18841 catagtgact attacatcat atatactttc atccttctta gctagtacag gcatgctcat
    18901 tatatcctct actgtttttt cttttacact tttaccaatt aaatttattt cactaactcc
    18961 tgaataaaaa tatccaactt ttggtcttgc gtctaatatt cctgtcattg ttaatatagc
    19021 taaatcagat ctaagtgtag cacgagtcac attcaagctg gaggcaatac tttcactcgt
    19081 tattggttca ttttccttaa ctatatctat aatttttagt tgtctttcat taagctgaat
    19141 aataatcacc ttctttcatt gtgctatact ttttatcttt tcgcatctta tatagtatat
    19201 tatataacta tatataccat tctgtaaaca gattttttat tgaatacgat atatttttta
    19261 ctaagttctc tcgcttaaag atatttatta tatattaatg ggggatattt ctttaattaa
    19321 tagaactata ttagta
//
LOCUS       DAMJWU010000066        18847 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMJWU010000066 DAMJWU010000000
VERSION     DAMJWU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.067"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(301..693)
                     /locus_tag="PC460_002490"
     CDS             complement(301..693)
                     /locus_tag="PC460_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2601370.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            1260..1622
                     /locus_tag="PC460_002491"
     CDS             1260..1622
                     /locus_tag="PC460_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601371.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1606..2313
                     /locus_tag="PC460_002492"
     CDS             1606..2313
                     /locus_tag="PC460_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601372.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDISNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEIINQFKNESENIRNQEVVEFTEEDEEELKQNIIENIK
                     KNFDEKNLVQVYKYRGV"
     gene            2333..2752
                     /locus_tag="PC460_002493"
     CDS             2333..2752
                     /locus_tag="PC460_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601373.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEVYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2742..3266
                     /locus_tag="PC460_002494"
     CDS             2742..3266
                     /locus_tag="PC460_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601374.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKCSQKYE
                     TDFKIKVWNFSESI"
     gene            3578..4135
                     /gene="efp"
                     /locus_tag="PC460_002495"
     CDS             3578..4135
                     /gene="efp"
                     /locus_tag="PC460_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF2601375.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4227..4682
                     /locus_tag="PC460_002496"
     CDS             4227..4682
                     /locus_tag="PC460_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2601376.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4845..5555
                     /locus_tag="PC460_002497"
     CDS             4845..5555
                     /locus_tag="PC460_002497"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF2601377.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5609..6679
                     /locus_tag="PC460_002498"
     CDS             5609..6679
                     /locus_tag="PC460_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2601378.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6707..10261
                     /gene="smc"
                     /locus_tag="PC460_002499"
     CDS             6707..10261
                     /gene="smc"
                     /locus_tag="PC460_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF2601379.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSTNLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10288..11568
                     /gene="ftsY"
                     /locus_tag="PC460_002500"
     CDS             10288..11568
                     /gene="ftsY"
                     /locus_tag="PC460_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF2601380.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPVPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11671..12039
                     /locus_tag="PC460_002501"
     CDS             11671..12039
                     /locus_tag="PC460_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF2601381.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            12036..13388
                     /gene="ffh"
                     /locus_tag="PC460_002502"
     CDS             12036..13388
                     /gene="ffh"
                     /locus_tag="PC460_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF2601382.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13426..13698
                     /gene="rpsP"
                     /locus_tag="PC460_002503"
     CDS             13426..13698
                     /gene="rpsP"
                     /locus_tag="PC460_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF2601383.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13763..13990
                     /locus_tag="PC460_002504"
     CDS             13763..13990
                     /locus_tag="PC460_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF2601384.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14098..14613
                     /gene="rimM"
                     /locus_tag="PC460_002505"
     CDS             14098..14613
                     /gene="rimM"
                     /locus_tag="PC460_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF2601385.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14622..15317
                     /gene="trmD"
                     /locus_tag="PC460_002506"
     CDS             14622..15317
                     /gene="trmD"
                     /locus_tag="PC460_002506"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF2601386.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15502..15852
                     /gene="rplS"
                     /locus_tag="PC460_002507"
     CDS             15502..15852
                     /gene="rplS"
                     /locus_tag="PC460_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF2601387.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16173..17069
                     /gene="ylqF"
                     /locus_tag="PC460_002508"
     CDS             16173..17069
                     /gene="ylqF"
                     /locus_tag="PC460_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF2601388.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17458..18750
                     /gene="brnQ"
                     /locus_tag="PC460_002509"
     CDS             17458..18750
                     /gene="brnQ"
                     /locus_tag="PC460_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2601389.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7967 a         1761 c         3466 g         5653 t
ORIGIN      
        1 cattaatatc tataaaatga caaaataaaa aatactattt aaatagatat taaaaaattg
       61 ataccaagta tttgtttgct atatgaataa caatttattt aggagcttta cttggacact
      121 gtataataat aaagtaattg ctaaaaacgt ttgatgattt ggatgtattt ttagaaatta
      181 gaaaaatata tacttactga ctaatgataa tcttatatgt attttagttt gagtgattta
      241 agaaaagtgt tttgaatggg gattatttta tagagtaaat aaatatataa tagtagaaaa
      301 ttacatataa agagtagtat ttatttcatt accttcttta tctgttccga tagggtcttg
      361 tagagatact tgagttttat tttttttgtt agatctaata ttcattaaaa tctcattttc
      421 aatacatcta gaagcatatg ttgctaatcg agttccttta tctatgttat aagtttctat
      481 agctttaatt aatcctattg tacctatgga tattaaatca tcctgatctt ccgttgaatt
      541 attatatttc tttgctatat aagctactaa tctcatgttt ctttcaatta atgtatcttt
      601 tgcactttta tcgttttcta ttttaaattt agttaaatac tcaatctctt cttcaggggt
      661 taagggtttt tcaaaagatt taagagctgc caataggtca cccccttttc aacggtcact
      721 ttaaattata tgaaggagac ttatccaagt tgactgttat ctaataaaat aatatgcata
      781 tttttagaat ttaaaacata tatttgcaaa cttttgaatg gaatatgaca taaagagtgg
      841 gaatttaaat ttaagttttt gttgtatata tatgaaacaa ggcgaagaaa ttattgattt
      901 ataaagtata aattggaatt gaggtgtcaa taaatataaa aggaaatagt atttatatat
      961 gttttggtgt tgaagtaaaa gattaatgca aagcattaga gtctataaaa atatgaatat
     1021 aggaggtgta attttacaat acttaaaaaa gtaaatgtaa ttaattgttt gtataaagtt
     1081 gattacaagc aaaagtatca gtcatagtta atactgttaa ggatgaaggt ggtgtgttaa
     1141 tttatatatg tatgggaaga aattttgatt acaatgaaaa aacttacaaa agatatttac
     1201 gaaaaaactt atagttaaat gtttatatat aaaatattat ttaggggttg tttttattat
     1261 tgaaaataag aaagagtggt tttatatcaa ttgaatgtat aataagtatt gctatattat
     1321 atgtggctgt ttatttagtt tctacatcat tgtataattg ttatagtttt atcagtagaa
     1381 atatatctga cagagaaatg ttaagtacag caaaaaaata tatagaagat gagaagtata
     1441 gaatacaaaa tagtaagtat gagttaattg aagataagat agaaaaaaat tacataaatg
     1501 gatatgaaat taacagtaga atagagcaaa ttttagatta ttatcaatgc tatgaaataa
     1561 atatagagat aaaaaatgaa tttaaaaaac tgaggtttaa tagctatgtt actagaaaat
     1621 aaaggctcta tattaataat aacattgata atattttcta ttataagtac tgtgtgcatt
     1681 atgtgtatag gtttaattta tacacataat aatatgtttg agttaggata caaagacatt
     1741 caactaaaag aaatttcttt aagtggaata gaaatatcag taagtaatgt actttcttgt
     1801 gttgaggaag ctattgatat atctaataat gaagacgaat ttagagaata tttcttagga
     1861 aataatatga ttgaatgtat aaaaaaaata aaagatacat cttatagtag tttagaagaa
     1921 ctttctttaa aagtagaaaa taatactatt tatgacagag gagatttttt tgaatttgat
     1981 atagaaagta atgttagaga aaaagaattt ttaaaagcaa taaaagtaag agtgaaaatc
     2041 aaaaatccat ggataggaat tgatattgaa gattatatgg ataaatctaa taacaataaa
     2101 attgatgaca aaacattcaa taattataaa gcatctgaaa ataatgaaat tataaatcaa
     2161 tttaaaaatg aaagtgaaaa tataaggaat caagaggttg ttgaatttac agaagaagat
     2221 gaagaagaac ttaaacaaaa cataattgaa aatataaaga agaattttga tgaaaaaaac
     2281 ctagtacagg tatataagta taggggtgta taactttgaa aaaagaagga atgtgactgt
     2341 aatagaatta atagtatcta tcagtataat actcttgata gttacattat cttttccaaa
     2401 agataatctt gaaaatcata aaataaattt atttgcacgt caactttgtt cagatataag
     2461 gtatgttagg cgaattaata tgtttagcaa ctacaataca tatatatatt atataaatca
     2521 aggtaaacac gaaggttatg tattgagaaa agatggtaaa aacattaaat caataatgct
     2581 acctaaaaat gcaaaaatag ctaggagtat tgaaataata aagtttaaat ctgatgggtc
     2641 gcctcaaaag gcgtctacaa tagaggtata taataataga ttaagaaaaa ctataacaat
     2701 aacacctgta agtggaagag tacttctgaa agagggaaaa tatgaaacat aaatatggtt
     2761 acttgctttt ggaaagtgtc gtatcattgt cttcaatggt gataattata ctagttttat
     2821 attctatatt tttgtctaca ataaacttaa agttaaaagt agaagataag atagaactac
     2881 aacaacaaag tcttgaaatc ataaaatcta tggaaggaat tattagcaat tctatgggaa
     2941 taatgaatgt atctaattat gaagaaacat ttaaaaagac aacatctata aaatgtagat
     3001 atttagatga aaataataat gaagaaggca taagtaataa agaaattata ttgaatgaaa
     3061 gacgaaataa gttatttgta aattctttaa atggcgaaag tagtcaagca ggtggttatg
     3121 aaattggaga ttatgtagat gaaatgtatg tatcaattac taataatggt caatacgtta
     3181 atattaaatt gaaattatca aaatgtagtc aaaaatatga gactgatttc aaaataaaag
     3241 tatggaattt tagtgaaagt atataaaaat acataaattt aagagaaaga aatgacatca
     3301 ataagtcata taaaatataa ttattgagaa ctatataact aaataatatc aatatgtaaa
     3361 gtgtatagat taagaatata gaggataata ataaaaatat agaataagta atttgccaaa
     3421 tacttgttta tattgtataa cttatgatat tatgatatta gtaaaaattt gtaatcctga
     3481 cttgacagaa ttcaagtaag agatataatt ttaaaggcat agtaatgtaa aatttactaa
     3541 aaaataagaa atttatttta atatggagga acaaaaaatg gtatcagcag gtgattttag
     3601 aaaaggtgtt acatttgaaa aagatggaca accatgttta gtagttgatt ttcaacacgt
     3661 taagccaggt aaaggagctg ctttcgttag aactaaatac aaaaacttaa aaacaggagc
     3721 tataagagaa gaaagtttca atccaagtga aaaattccct aaagctgtta tagatacaag
     3781 acaaatgcaa tatctataca atgatggtga gttatattat tttatggatc aagaaaattt
     3841 tgaacaaata ccattaaact atgagcaagt tgaagatgca attaagttct taaaagaaaa
     3901 tgaagttgct acaataagat tttaccaagg acaaccattc caagtggaag cgccaaactt
     3961 tgcagagcta gaagttacag atacagagcc aggcataaaa ggtgatacag caagtaatgt
     4021 aactaaagca gctacagttg aaactggagc agttgttcaa gttccattat ttataaatac
     4081 tggagataaa gtaaagatag atactagaac aggtgaatat ttatcaagag tataaaaatt
     4141 tatgtatata aaagcttata tgtcttaata atataagata tatgagcttt tagtttatat
     4201 ataaaaaaga aagcgagggg tgttacatga aacatttata tgaagaagtt gcatacttaa
     4261 aaggcctagc agaaggacta gaaataagtg ctgaaagtaa agaagggaaa atgatacata
     4321 aaatagttga tgcattagaa gtatttgcag atgctattgt aacattggat gaagaacaag
     4381 aagaacttca agattttgtt gaatctatag atgaagattt agcagattta gaagaagata
     4441 ttgctgacat ggaagaagat ctttatgaag aagatgacga tgaggatgat gaggacttca
     4501 gctatataga aatggaatgc ccaaattgtg gagagttagt agaaatagac gaagatttgt
     4561 tatatgatga tgaagtagat gttgtttgtc ctgattgtaa ggctgtgata ttatcatcag
     4621 aagatgattg tgatgatgat tgtacatgtg gtggttgttc aagttgtgat gatagagagt
     4681 aaaaattcat aagaattaaa actgcctatg atgcctatga gatattttgc ttcataggta
     4741 gttttatttt tatgaggaag ggtattatgt attttggttt tgcaatactt ggacaaaaat
     4801 agtacactag aatttataga ataggatttt ggagggatgt aaatatgaaa ataagtaaaa
     4861 aattactaga taatatacaa agatttgaaa atgttataaa ttataaattt aaaaataaag
     4921 aatatatact agaagctctt actcatagtt catattctaa tgaaaataaa aaatataatt
     4981 ttaacgaaag attggaattt ttaggggatt cggttttagg catagtgata agtgattatt
     5041 tatttaatga agaagctaat ctgcccgaag gggaactaac taaacttaga gcaaatatag
     5101 tctgtgaaga ttcattaagt gaagtagcta atgatataaa tttaggtatt cacatgttat
     5161 taggtagagg agaagaagca acaggaggaa gacataggac ttcaatatta gcagacgcat
     5221 ttgaagctgt aatagctgct atatatcttg atggaggttt tgaaagtgca agacagttta
     5281 tacttcatca tatggaaaat ataatttatg attcaagaaa aggaaatata tttagagact
     5341 acaaaacgca tcttcaagag gtgttacaag ggaatggaga aaataatatc tggtacaggc
     5401 ttatagaaga aaaaggacca gaccataata aaagatttgt aatggaagtt ggaattaatg
     5461 acgatgtact tggtatagga gaaggcaaga gtaaaaaaga agctgaacag ttagctgcta
     5521 aaatagcttt aaagaaaaaa ttatgggaaa aataaatata atatgtaaaa atataaaaag
     5581 aaatatttat attagaagga tgttgtagat gaaaaagaga attataccta tatttgttcc
     5641 tcacaaagga tgtcctcatg attgcatatt ttgtaatcaa aaaaaaataa caggtgtgag
     5701 tactgatgtt actagtgaag atgcaagaaa tataattgag gaatgtttag agactataga
     5761 taaagatgca gatgtagaaa tagcattttt tggtggtagc tttactgcta tagatattga
     5821 tatacaaaaa agtttattat cagttgctaa agaatatgta gaaaaaggtc ttgtaaaaga
     5881 tattagaatg tctacaagac cagattgtat tgatgaagat atattaagta tgttaaaaga
     5941 atataagaca agtataattg aactaggagt acaatcctta gatgaaaaag tattattaga
     6001 tagtataaga ggtcatcaat ctgaaattgt atacaagagt tcaaagatga taaaaaatag
     6061 tggaatcaaa cttggattac agatgatgat tggtcttcca gctgacacag aagaaaagtg
     6121 tatatttaca gcaaaaaaat ttgtagagct aaaaccagat tgtgttagag tctatccaac
     6181 tttggtagta aaagatacag gtcttgaaaa gttaatggaa caaaataaat ataatccatt
     6241 tacccttgaa gagagtgtaa atatagtaaa aaaagttctt gtactgtttt atgttaataa
     6301 tatcaatgta ataagagttg ggcttcaagc tactgatgat atacaaattg gaaaagctgt
     6361 tttagcagga ccatatcatc ctgcatttag agagttggta gaagcagata tgataaaaga
     6421 ttatttagaa tttgttattt tgcaaaataa aaatataaag caaatgttgg tcaaatctaa
     6481 taaaaagaat atttctaaaa tcataggaaa taaaaaaaca aatgttaaat atatggaaga
     6541 aaaatttggt gtgcttttaa aaacacaaga atcagattta gatataaatc aattagaaat
     6601 tgtgttagat gggaaaagtt taattagtgc taatatgaga gacattcata gaaaattgta
     6661 tgacatttat gacctttagt tttaataatg aagggagaga aaagatttgt atttaaaaag
     6721 actagagtta aagggattta aatcctttcc agtaaaaaca gatattattt ttaaagaagg
     6781 aataacagcc atagtaggac caaatgggag tggaaaaagt aatatatctg atgctgtaag
     6841 atgggtatta ggagaacaga gtattaagag tcttagaggt gataaactcg aagatgtaat
     6901 atttgcaggt acagatacaa aaaaacctat gaattattgt gaagtggctc ttactattga
     6961 taattctgaa aatcaattgg aattagattt tacagaagta actataagaa gaagggctta
     7021 tagaaatggt gaaagtgaat tttttctaaa taataaaagt tgtagattaa aagatataaa
     7081 agaagtcttt ttagatactg gtattggaaa agatggttat tcaatcatag aacaaggaaa
     7141 agtagatgaa atattaagta ataatccttt aagcagaaga aaagtttttg atgaagcttg
     7201 tggcatatca aaatatagat ataaaaaaca ggaagcagaa aggaatttaa gtaatacaaa
     7261 ggaaaattta gaaagaatag atgatgtata tatagaaatt gaaaatcaat taaagcctct
     7321 ttttaatcaa cagacaaagg caaaaaagta cttggaaata agtgaaaaat taaagacact
     7381 tgaagtaaat agctttataa gagaaattga aggaatagaa aaagaattaa gtgaggttaa
     7441 tgaacatcgt aaggttattg aaaaggagct caatgaaaaa gaagaacaaa aaaatgttgt
     7501 agagaaaaag caagaagata ttaataaaga ggtagaggta ttacaagatg taattgaaaa
     7561 atctgtagat tatataaatt caattaaagg agttatatca aaaaaagagt cccaaataaa
     7621 tttaattaag gaaagaataa gaaattttac gaatgaaatt agtagaaaaa atttagaaat
     7681 aaatgatata aaagagaagc taaatgaaaa taagcaatac ataaaagaat tagaaagcaa
     7741 taaattatca ggtagtgaag aactatctac cttgcaggaa aatataaaag tattggaagc
     7801 aagtaaagat aaacaaaaaa ttaagttaga atccctaaat aatgagatag aattattgaa
     7861 agaaagtata attgatatat taaataaaaa acaagagttt agtaataagc tgtccacttt
     7921 aaacgcaaat aaagaaaata tgaatatcag agatgaaaat ataaattcag agataacaga
     7981 gttaaacaaa aatattgaaa taaaatcatc tgaattagat actataaata aagaatttaa
     8041 tatgcaaaat gaaaatttaa agaatgtaaa taatagacat aaagaattat caactaattt
     8101 gcaggattca attagtgaac acaataaact agaagatgaa atacagaaaa gtaaatataa
     8161 tttaaatgga tataattcaa agttaaatgt ctatatagat atggaaaatc attatgaagg
     8221 atttaataga ggagtaaaag aagtactaaa aaataaaaat ttaaaaggag ttcatggagc
     8281 attaggacag attataaatg tacctgaaaa atatgagaaa tctatagaag cagctcttgg
     8341 tgcttatatg caaaatataa taactgataa tgagtttagt gctaagtctg caataaacta
     8401 tctaaaacag aataatttag gtagagtaac atttcttcca ttaaatataa ttaaatcaaa
     8461 taaaataagt ctaggaaatt taaaagcaaa tactaagttc ataggaatag caagtgattt
     8521 aattacattt gatgaaaagt atagaaatat aattgaaaat atacttggaa gaacaatact
     8581 tataaataat atagatgagg gcataaaatt tgcaaaagaa actggtcata ggtttaaaat
     8641 agtaacttta gatggtgaaa tcttaaatcc aggaggttct ttgactgggg gaagtctaaa
     8701 aactaatggg aatatactat ccagaaaaag atacattaat gaatatacag aaaagataag
     8761 taatatcaag aatgaaattt cacatctaga attaaaaaga gaatctttag acaaagatgt
     8821 gaaaaatatc aaaaatgaaa ttgattcaca tgaaagtaaa ataaaagatt tagaaaaaag
     8881 tataataata aaaagtacaa gtataaagaa tgttgagtct gaaattgaat cgttaaaagg
     8941 tagtattact aaattagaaa atgagaagaa tgatttaaat tctaacctaa attatacact
     9001 tgaaaaaagt gatgatgtta gaaaagatat ggaagagtta gatgatttat acaataaaaa
     9061 taaagaaaaa attgatgcat tgaatgagga aataaaaaga tacaatgatt tatatgataa
     9121 agaaaaatct gaatttgatg aattgaactt aagtttagtg aagaaaacag aagtttacaa
     9181 tagtatagtt agagatataa aaagaatatc tggtgaaaat tgtgagctag aagaaaaaaa
     9241 taaacaatta gaagagtcat taaattatga agaacatgaa ataattaaat tacaagattc
     9301 tatactcact gaagaaaaag aaaaagaaaa tttaacaaaa caactagggg atagtaatag
     9361 aaatcttgaa actagaaaaa tagcaaaaga tgatttaaaa aatagttttg atgaaattaa
     9421 taaagaatta aaaactatag atagacagca tatagaactt aaagaaagtt tatttaaagt
     9481 tggtggaaga ttagaaagat taaaaactag tcaagacaca tatataaata agttgtttga
     9541 gcaatatgat atgaccttag tacaagcatt ggaaattaaa gatgaagatt tggatataga
     9601 tagaaaattc ttagaaagtc taaagagaga aataagaagt cttggaaata taaatataga
     9661 ctctataaag gaatatgagg aaataaaaga aagatacgat ttttatagcg aacaaaagca
     9721 agatttagaa gagtctatgg aagagataga aaaacttata catactttag aagaaaatat
     9781 gaaatctgaa tttgaaatta agtttgaaga aataagtaaa aattttaaat atgtatataa
     9841 gagattattt ggtggaggtt gtggagaatt aactatttta gataaagaaa atttattaga
     9901 aagtgatata ctaataacag ctcaaccccc aggtaaaaaa atgaaaaatt taaacctatt
     9961 atctggtgga gagaaagctt taacggcaat aagtatatta tttgctatat tgattacaaa
    10021 gccaacacca ttttgtatat tagatgagat tgaagcgcca cttgatgacg caaatatatt
    10081 tagatttgga gaatttttga aggatttatc taaagagact caatttatat cagtaactca
    10141 taggagagga actatggaag ctgctgatta tatctacgga gttacaatgc aagaaaaagc
    10201 catttcaaaa gttattagtt taaaattaaa agaagctcag gaaataacag atattatata
    10261 acttagattg acataggagg aataacagtg ttaaaaaaat tatttggttt tggaaaagac
    10321 aaggaaaagg aaatagaaaa aaaagatgct gaagaagaaa tagaagttga agacagtgtt
    10381 gataatttag aaaacttgga agaaactatt ttttcaggac ttgaagaaga agtgatagat
    10441 aaagtggaag atgttgaaga aaaaaatgag gaaagtgtca ataatgaagg cactgaagaa
    10501 atcgaaaact ttgaaaaaat agaagttgag atggatagta atggaaaaga agtagaaaat
    10561 atttcaaata atgattctca agaagagata atcgaagaat tagaaaatta tgatgaaatt
    10621 gagtctaaag agatagaaga taaagaagat aagaaagtta acttgtttga aagactaaaa
    10681 caaggattga caaaagcaaa gcaaggaata actgatagaa tagatgaagt tttaaaatca
    10741 tatacaaaga tagatgaaga attacttgaa gatttagagg aaattttaat aacagcagat
    10801 gttggtgtaa atacaactat ggatataatt gaaagactaa gagataaaat aaagcaaaaa
    10861 ggtattactg aaccaataaa ggtaagagaa gaacttaaat ctatagttga agatattctt
    10921 acaaatgaaa actctacact agatatagaa ccagtaccat gtataatatt aatggtgggt
    10981 gtaaatgggg tcggtaagac tactacaata ggtaaactag caaatagata caaaaaagat
    11041 ggtaaaaaag ttttattagc agcagcagat acatttagag cagcagcaac agagcaatta
    11101 gaaatttggg caaatagaac caatgtagac ataataaaac atcaagaggg agcagaccct
    11161 ggagctgtag tatttgatgc tataaaagct gcaaaagcta gaaaaacaga tgtattaata
    11221 tgtgatacag caggaagatt acacaataag gctaatctta tgaatgaact tggaaaagta
    11281 tttaaaatag tagatagaga atttccagaa gcaaaacgag aagtacttct tgtggtggat
    11341 gctacaacag gtcaaaatgc agttgttcaa gcaaagacat ttaaagaagt tgcagatata
    11401 acaggtatag tacttacaaa acttgatgga actgcaaaag gtggagtagt ccttgcggta
    11461 aaatcagaag tagatgtacc tgtaaaactt attggagttg gagaaagtgt agaagaccta
    11521 caagatttca atgcaaaatc attttcagat gcattatttg gtaactaatg ttcttttaaa
    11581 aataatggtt gacaagagtg ttgttatgat ataaaataca atagtgtaaa gtaaataacc
    11641 tttacagtat actgctagga agtgtaagat atgaatatag aaaaaatggt cgaaattgga
    11701 ttgttatttg aacagtataa agaattatta actgacaagc agaaagaaat agttgctcta
    11761 tactatgaag aagattattc tttaggtgaa attagtgaaa atctgaatgt atcaagacaa
    11821 ggtgtttatg ataccttaaa gcgttcagaa aaaatactaa gagattatga agaaaagtta
    11881 cacttagtat caaagattca agagcaggaa aaaaatatta agattataaa agataaaatt
    11941 attgacatta aagaagattt attgcacaat agagattgtg ctaatttaat ccctaagcta
    12001 gaaaatatag aagatgtatg tagggagatg ataaaatgat atttgaagga ttgtctgata
    12061 aactacaagg ggcatttagt aagttaaaat ctaaaggaaa acttacagaa gtagatgtaa
    12121 aaaatgctat gagagaagtt aaacttgctc ttcttgaggc agatgttaac tttaaagtag
    12181 ttaaagattt cataaaaaaa gttcaagaga gatgtgttgg gcaagaagtt atgcaaagct
    12241 taactccagc tcagcatgta atcaaaattg taaatgaaga gcttacaagt ttaatgggag
    12301 atgtacaaag taaagtaatg atttcctcaa agcctcctac aattttaatg atggttggat
    12361 tgcaaggtgc aggtaagact actacagcag gaaaacttgg aggatatttt aaaaagcaag
    12421 gtaaaaaacc tctattagta gcttgtgata tttatagacc agcagctata aagcaacttc
    12481 aagttgttgg agaaaaattg gatatacctg tattcaatat gggagataaa gaaaatccag
    12541 taaatattgc gaaagcaggt ttaagccatg cgattaaaaa tgctaatgat ttagttatta
    12601 tagatacagc tggtagactt catatagatg aagtacttat ggatgagtta aagtctatta
    12661 aatcagaagt taagccacat gagatacttt tggttgtaga ttctatgaca ggtcaagatg
    12721 ctgtaaatgt tgcagaaagc ttcaatgagg cacttggtgt tgatggagta gtattaacta
    12781 aattggatgg agatactaga ggaggtgctg cactttcaat aagagcagtt acacaaaagc
    12841 ctataaaatt tatggggatg ggagaaaagc tagatgatat agaacctttc cacccagata
    12901 gaatggcatc aagaatatta ggtatgggag atgtcttaag tttaatagaa aaagcacaag
    12961 aaaatataga tttagagaaa gctaaagaat tagagcaaaa aattaaaaaa caagagcttg
    13021 attttgaaga tttcttacaa caaatggaac aaatacaaaa tatgggacct cttgacaaga
    13081 tacttggtat gatacctggt atgggaaatg taaaagacca acttggagat gtagatttga
    13141 atggcaaaga aatgaagaga acaaaagcca taattcagtc aatgactaca tatgagagaa
    13201 gaaacccagg agtattaaat gcttcaagaa agaaaagaat agctaaaggc agtggaacta
    13261 gtgtacaaga tgtaaataga cttataaaac aacttaatga aatgaaaaaa atgatgaaga
    13321 tgtttgctgg ttcacaaaag agtatgaaga aaaggggcgg cttaggcggt ttaccttttt
    13381 ttaaataaaa tataaaaaag taaaaattat tcggaggtga ctgtaatggc agttaaaata
    13441 agattaaaaa gaatgggagc taacaaaaaa cctttctaca gaatagtagt agctgattca
    13501 agagctccaa gagatggaaa atttatagaa gaaataggat actataatcc aatttctgaa
    13561 cctaaacaag taagaataaa tgatgaaaaa gctataaaat ggttagctac tggtgctcag
    13621 ccaacagaag tagttaaaaa attacttgta aaaaacggag taatagaaaa attcgaagct
    13681 tctaaacaag caaaataatt ttttcttggc aattagttta agtaagggct cattgctaag
    13741 gttactttag gagatgaaat atatgaaaga gctagttgta gatatagcta aggctctagt
    13801 tgataatcct gactcagttg ttgttgagga atttgaagat aatgatggta tcgtcttaaa
    13861 gcttacagtt gctcaagagg atatgggtaa ggttattggg aagcagggaa gaatagctaa
    13921 agctataaga actgtcgtaa gatctgttgc aaatagagaa aatataaaag tttctcttga
    13981 gatagtataa aataggagga ttaggtataa tgcctagtcc tttttatata aatgcgtagt
    14041 ttaagatttt atgatgatat gaccacataa gattaaattt tttaagaagg tgataacatg
    14101 aaagaaaaat taactcattt taaggtaggt caaatagtaa atacacaagg tttaaaaggt
    14161 gaagtaagag tttatccgct tactgatgat atagagaggt ttgatgaatt aaaagatttc
    14221 tatctgggaa aagatttaaa taataaattg gctgttgaaa aagtaagata caaaggttct
    14281 atggttataa tgaagataaa aggcatagac agcatagaga aagcagaaaa actaaaaaat
    14341 aaatttatgt atgtatctag agaagatagc agagagcttg aagaaggcga attttttata
    14401 gcagatatga ttggaatgga agttttaact gtagatggaa aatatgtagg aatactagaa
    14461 gatgttcttc aatactcagc aaatgatgtc tatgtagtaa aaggtgagga agataaagaa
    14521 tttatgatac cagctataaa aaaatttgta cctacaatag atattgatga aagaaaaatg
    14581 attatagacc ctattaaggg aatgatagat taggtggtaa tatgagattt catataatga
    14641 cactttttcc agaaatattt aattcgtaca tggatgaaag tataatgaag agagcagttg
    14701 aaaaaggtat aattgaggtt catatatata atataaggga tttttctaat aataaacata
    14761 aaaaagtaga tgattatcca tttggaggag gagcggggat ggtaatgact cctcaaccta
    14821 tatatgacac atataagcat ataataacaa ctcataatat aaataaccca agtgtaattt
    14881 atcttactcc aaaaggtaag gtatacaatc aatctatggc aaagcagatg tctttaaaag
    14941 aagatataat actactatgt ggtcattatg aaggaataga tgagagaatc attgatttaa
    15001 tagttactga tgaaatttca attggagatt atgtattaac tgggggagag ctcccagcac
    15061 ttataatgat agattctata tcgagattaa taccaggtgt attaaaccaa gaagaatcat
    15121 ttgaagaaga atcatttaaa gacaacttat tggaatatcc tcattacact agacctagag
    15181 attttgaagg attaaaagta ccagaagtgc ttttatcagg gaatcataag aagatagatg
    15241 agtggagaag agaagaatct ataaggataa ccaaagaaag acgatttgat ttatataaaa
    15301 agtctaatga aaaataaaaa tataatgaaa aattaatata tatgtataca ataaagtacc
    15361 tataaatgga attaaaaaca taaaagtgct tgtaataact attgaagtat agtataatat
    15421 aaaatgttga aaatacgggc gttcctctgt ttacactggt cgggtagtta agaacgtcta
    15481 tgatatctag gaggaagaaa gatgaatgaa atgctaagag caattgagca agaacaatta
    15541 aaaaatgaag ttcctaattt tggacctggg gacacagtta aagtacacgt tagaattata
    15601 gaaggaaaaa gagaaagaat acaagtattc gaaggtgtag tattaaagag acaaggtgga
    15661 ggagcaagag aaactttcac agttagaaaa atgtctttca atgttggtgt agaaagaact
    15721 ttcccagttc actctccaaa aatagaaaaa atagaagtta ctagaaaagg taaagtaaga
    15781 agagctaaac taaactactt aagaggtaga gtaggtaaag ctgctaagat aaaagaagct
    15841 agaaataaat aattaaaaac tttacttata aatggggggc tgatttatca gtcctctttc
    15901 tattttaatg aaaattacaa caaataatta tactagaaat aaatagaaat atttgttgaa
    15961 aatatagatt atttatctat gaatttatag cttttaaaat gaaaaataaa attggtaaat
    16021 attgcattat attttgtaac gatattaaaa ataagagaac agtattatgt aaagtatgac
    16081 gttaaaaaga tgacaatatt atagtttgtg aagatatatt ttttaagtgg aattaggata
    16141 taataatata atattaataa ggagtgatac atgtgtcaaa tgaatacgaa gagtatctta
    16201 tggatgacaa tctacatata aactggtatc caggtcatat gaaaaaaact aaggagttag
    16261 taaaaaataa cttaaaattg attgatgtag taattgaact tttggatgct agaatcccgt
    16321 ttagtagtaa aaatccagac atagacaggc ttgtaggaga taagccaaga gttgtcgtac
    16381 ttaataagag tgatatggca gatagggata aattgaatca atggattgaa tactataaga
    16441 aaattaatat caaagctata ccagtagata caataaaagg tgttggtata aataagataa
    16501 tcgaagaatg taaaaatgta actagagaaa aaatgagttc tttaaaagac aaagggagaa
    16561 aagaaagagc tatacgaata atgatagttg gcgttccaaa tgttggtaaa tcgtctctta
    16621 taaacaaatt aacaggtaga aaaagcactc aaactggaga taagccagga gtaacgaaag
    16681 ggaaacaatg ggttagatta aagggaaatc ttgaactttt agatacgcct ggaatacttt
    16741 ggcctaagtt tgaagaccaa gaagttgcat taaatttagc atttagtaga gctataaagg
    16801 atgagatttt agatactgaa actttggctt taagacttat agaaaaactt atgaaaatag
    16861 aacctgagaa gttgaaagcc agatataagt tagattgtct aggagaaact ccaattgaaa
    16921 ctatggatat gattggtcat aaaagaggat ttataacagg taaaaaagaa ctagattata
    16981 cacgcatagc cacaactgta ttaaatgaat ttagagatgg aaaaatagga aatataacat
    17041 tggaagttcc agaaaacgtt aaaagataga ttttaaaaca aaatcaaaaa tttaatgtta
    17101 aaaatttaaa aaagcctttt agcaaaggct tttttatttt tttgaaatat agacaaatat
    17161 ctccatataa aagtataaat taggttagaa agcctttaaa aatattaatt taagacaaat
    17221 atacctaata ttttgaaaat taaaatgagt gtgattatta aaaattttag catattgact
    17281 tgcttgtttc gtataatata taataagcac aaataaatac aatatattaa aaatttaatt
    17341 tatttaattt tattaattta ataataagtt cttattgttt aattcaactt acttatgcaa
    17401 gcgtaaacaa tcgagacatt ttattaattt aataaggtta aaaaggggga ttttataatg
    17461 ggtaagacca aggatgttat agtatttgga tttgcgttat ttgcaatgtt ctttggagca
    17521 ggtaacttaa tatttccacc ttacttggga ataattacag gtccagaatg gttaattgcc
    17581 ttcttaggat ttacgtttgc tgatgctgga ttggcacttc tagcagtaat ggctacagct
    17641 aaattcgatg gaaacgtagt tgaaatgttt aaaagatgtg gtatgaagtt agggattctt
    17701 ataggttgtg cagatatttt atgtatagga ccattcttag ctataccaag aacaggtgca
    17761 acaacttatg aaatgggtat aatgccttta tttggaactt caattccagt gttattattc
    17821 tgtatacttt tttttgcgct ttcatatgta ctaacaataa gaccttctaa ggtcgtagat
    17881 atagttggac agtttttaac tccagctctt ttaatagctt tagcatttat aattataaaa
    17941 ggtattatat cacctcttgg agatatagta gataaaccta tgatacctaa tgtatttgca
    18001 gaaggtatag gtcaaggata tcagacaatg gatgcttttg cagcaatagc acttgcatca
    18061 gttctaatag tatctttaaa tgataaagga tattctacta ttagtgacaa gttaaaaatg
    18121 attggtaagg cgggtgttct tgcttgtgga ggattagctc tagtttatgg aggattatgt
    18181 ttccttggag caactgtatc aacaatgtat ggaacagatg cagtacaatc acaagtaata
    18241 gtaaatataa ctgaaggatt gcttggaaat gttgggaaag cgattttagc agtagtagta
    18301 tcattagcat gtttaactac atctataggt cttacatctg ccacaggtca atatttctct
    18361 agacttacaa aaggtaagtt gagttatgaa aaaatagtat tagctgtatc tgtattcagt
    18421 gcagtagtag catcttttgg tgttggaact ataataaaga tagcatctcc tatattaagt
    18481 atagtttatc caccatcaat tgttttaata attttagctt tcttcaatga aaaaattaag
    18541 aatgataatg tgtacaaagg agcagtatat atgtcattgc ttgtaagtat actaacagtt
    18601 atatctagtt atggagtagc tgttccagta gtaaactctt tacctcttaa ttcattagga
    18661 tttaactggg tggtgcctgt tataatagct ggaataattg gcaattttat accatcaaag
    18721 agtcaatcta atacacttgg aacaaactaa gttttattta attaaaatat aatcaattgt
    18781 aaataaatga gttaactaaa agtaaaagga gttatttgta caaataactc cttttatttt
    18841 tagattc
//
LOCUS       DAMJWU010000067        18818 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMJWU010000067 DAMJWU010000000
VERSION     DAMJWU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.068"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            776..2446
                     /locus_tag="PC460_002510"
     CDS             776..2446
                     /locus_tag="PC460_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF2601390.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHKMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESSKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2568..3302)
                     /gene="pflA"
                     /locus_tag="PC460_002511"
     CDS             complement(2568..3302)
                     /gene="pflA"
                     /locus_tag="PC460_002511"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF2601391.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3462..5693)
                     /gene="pflB"
                     /locus_tag="PC460_002512"
     CDS             complement(3462..5693)
                     /gene="pflB"
                     /locus_tag="PC460_002512"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF2601392.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5986..7047
                     /locus_tag="PC460_002513"
     CDS             5986..7047
                     /locus_tag="PC460_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF2601393.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7138..8751
                     /locus_tag="PC460_002514"
     CDS             7138..8751
                     /locus_tag="PC460_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2601394.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9026..11011
                     /locus_tag="PC460_002515"
     CDS             9026..11011
                     /locus_tag="PC460_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2601395.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            11016..11450
                     /locus_tag="PC460_002516"
     CDS             11016..11450
                     /locus_tag="PC460_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF2601396.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11479..12039
                     /gene="srlA"
                     /locus_tag="PC460_002517"
     CDS             11479..12039
                     /gene="srlA"
                     /locus_tag="PC460_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2601397.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12103..12471
                     /locus_tag="PC460_002518"
     CDS             12103..12471
                     /locus_tag="PC460_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF2601398.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12496..13062
                     /locus_tag="PC460_002519"
     CDS             12496..13062
                     /locus_tag="PC460_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2601399.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13103..13468
                     /locus_tag="PC460_002520"
     CDS             13103..13468
                     /locus_tag="PC460_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2601400.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13569..14342
                     /gene="srlD"
                     /locus_tag="PC460_002521"
     CDS             13569..14342
                     /gene="srlD"
                     /locus_tag="PC460_002521"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF2601401.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14691..17129
                     /locus_tag="PC460_002522"
     CDS             14691..17129
                     /locus_tag="PC460_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2601402.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDMALHKVNYVSDELDTTSNKVNYVSDELDMALHKVNGVSDELDMA
                     LHKVNCVSDELDMTSNKVNYVSDELDMTSNKVNYVLDELDITSNKTDYISNELYNLSN
                     KSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTVTLCDMFIP
                     CTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKR
                     TYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKNLG
                     ISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINQRMAKKVG
                     IEEKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQ
                     VTYNESMVEIIRQN"
     gene            17217..17552
                     /gene="spoIIAA"
                     /locus_tag="PC460_002523"
     CDS             17217..17552
                     /gene="spoIIAA"
                     /locus_tag="PC460_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF2601403.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17566..17994
                     /locus_tag="PC460_002524"
     CDS             17566..17994
                     /locus_tag="PC460_002524"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF2601404.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            18017..18781
                     /gene="sigF"
                     /locus_tag="PC460_002525"
     CDS             18017..18781
                     /gene="sigF"
                     /locus_tag="PC460_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF2601405.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
BASE COUNT         7335 a         2133 c         3252 g         6098 t
ORIGIN      
        1 attttgtagt gtaatcgtat tttttattaa ttcttacaaa atcaatgaaa ataagtatat
       61 ccattttaaa gtacataaat ttaaaaatcc agaaaaatat acttaaatta tgtaagtaaa
      121 agaggggttg aatcagttga aaatgtaata ttagcaaata gtacatcaat cctatatacc
      181 ctgtaagcaa ctctattttc atgtactaaa aatgtaccta ttttattaac tgaaaagaat
      241 aaattaaatg taagtacata agaagataga aaaaattaaa aacaaaaaat atttatataa
      301 ataaatataa aaaatgatat aatccctatt aaaaaattta tagatgatga agaaatatca
      361 aaatcttata ttataggaag agacacttct ttgccaaaat ctatagaaaa acaataccaa
      421 atccaaagag aataggcgga gaaaaaaata aaataaattc aagagtaata gaaaagtttt
      481 atagaataaa gaactgataa ctctttatat aacgaaaaat agaattttaa gagaaaatga
      541 tttaataact gatttaacat ggtgttttag caggtaaaaa taaatcacca gtaatgatta
      601 tagaaaaatt actttatgaa tctcaaaaaa cttagttaag agtaagagat ttgataagat
      661 aactaaagtt ggggaaatgt gtttgatgag acaaaaaatt agtaaaatag tataatctaa
      721 ataaggatat tttgttgatt aaactacact aatagtaata ttgtaggggg aaaagatgag
      781 agaagaatcg aactctttaa gtttagacca tatagaaatt ttctttgaac ttttatctca
      841 aagtacagaa gattatatat ttttttggga tataaatagg aataaattta aaatttcttc
      901 agcaattttt gatgaattta acttaagtaa agagatagaa tctgatttgg tcaattgttg
      961 gagtaaaatt gtttatccag atgatgttca aatatggaaa gatgatatac aagaattact
     1021 acatggaaaa aaaggtgagc acaatttaga atataggttg attaataaat ataaagaaat
     1081 agtatggatt tcttgtagag gtaaagttta tgtgtcggat gacccaaaaa ctatatttct
     1141 agtcgggaga ataaagaata ttggtgaaaa aaataaattt gattctatca caggaacgtg
     1201 gaatagagaa caatttgaac ataaaatgaa ttatcttata aaagagaaaa tttataaaaa
     1261 tggtgctatg tttattatgg atattgataa ttttaaaaat ataaatgaaa aatatggaca
     1321 ttcttatggt gacaaagtgt tgcgtgcaat tgctactgaa gtactggaat atcttccaaa
     1381 agatgtaaga ttgtataggt tagatggaga tgaatttgca tttttttacc ctatgtgtac
     1441 taaagaaact atagagaaaa tatatgaaaa gatacagatg tatacaaata ctcagcatga
     1501 aattgaatca agtaagtact attgcacagt gactgcaggc gttgctatgt atccagaaga
     1561 tggagataat tatttagatt tatttaaaca tgctgatatt gcattggata tagcaaaaat
     1621 tagtggtaaa aatagaatta agtttttttc acaagagctc tatgaaaata aactaaaggt
     1681 aatttcaatg caacaaaaac taagagagtg cgttgaaaat aattttaatg actttgagct
     1741 gttttttcaa ccccaggtaa atgctgttac aaaagaggta ataggggcag aggtactttt
     1801 aagatggcat tcgagtactt atggagaagt atctcctgtt gaatttatac caatactaga
     1861 acagagtaat ttaattatac cagttgggaa gtggattata aaagaagctg taaaacagtg
     1921 taaagaatgg cataaaataa atccagactt taaaatatct gtaaatgtat cgtatattca
     1981 gttaaaggaa gattttttta gagattttat agtagaatgt ttagtagagt atcaattaag
     2041 acctgaattt ttaattttgg aattaacaga gaattgttgg ataccagata taaatttact
     2101 aaatgataaa tttattagtt taaagggtat aggagtatat attgcaatag atgactttgg
     2161 tacaggatat tcatctttaa attaccttaa agaactgtct gttaatataa taaaaattga
     2221 aagaagtttt gttaagaata ttacatataa tagttatgaa tatacttttc ttgaatatat
     2281 tattaaatta gctcatatta taaatttaaa agtttgtgtt gagggaatag agtcttatga
     2341 agagtatgat atagtaaaaa gcttaggggt agatattatt cagggttttt tatttggaag
     2401 acctgtaagt gcctctgaat tttataaatt tcagctatca aagtgatgta tataaaatga
     2461 gatatatagt atataaaaat agggcaaagt ggaagttttg aagtttatta aaaatttaca
     2521 ctttacccta ttttgttatg tcttctataa aggattaata tactacacta actattatat
     2581 actttcactc caaatttaga gattatttca ctagcttttt ggacatcttc cttagtggca
     2641 tcaggaacat cttttagttc ataatcgata cctaaacttt cccatttgtg tattcccata
     2701 gaatggtatg gtagcaattc aaatctttct acatttttca atgtagctac aaattgacct
     2761 agtttttcta aattatctat atcatcagta atgccaggaa ctaaaacatg tcttatccat
     2821 acaggaatat ttcttgattc taagtgtcta gcaagtttta atgccttatc cataccaaca
     2881 ccagtcaaat caatagattt ttcctcaacc atatgtttta aatcaagtaa aaccaaatca
     2941 gtattatcta aaactgggtc tatagtttct atatctacaa atccagatgt atccaagcaa
     3001 gtgtgtatat cattttcttt tgctttttta aataattcac ttaaaaattc tggttgaaga
     3061 gtggattcac caccagaggc agttatacct ccaccagaaa acttcataaa agaagtatac
     3121 ttaagagcat ccgttattat ttcatcaact gtgtattctt ttcctgattt aatatcccat
     3181 gtatccctgt tatgacagta tttgcatcta agaggacacc cctgaaaaaa taatatatat
     3241 cttattcctg gtccatctac agtaccaaac gtttctatag aatgtatctt tcccttaatc
     3301 atagagttca cctcacaata tattttaata accttatcaa tagttataat atttctctaa
     3361 aaaaggccat cttataccag aaataattca aatataagat agccttttat ttttattttt
     3421 tacacagtag taatcttagt taaagtataa aaatttaaag actaagccat tttaccgtgg
     3481 aatgttctgt ttataacatc taattgttgc tctttagtta attttatgaa gttaactgca
     3541 tatccagata ctcttatagt taattgtgga tattcttctg gatgttccat agcatcttct
     3601 aaagtagctc tatcaaatac atttacattt aagtgatgag cattttgagc gaagtatcca
     3661 tccatcattg cagaaagatt gtttattctt tcagtcatat cttttcctaa agcagcaggt
     3721 actatagaga aagtattaga tataccatct tgagcatgtt catatggtaa tttagcaact
     3781 gaagataatg aagctaaagc accattagta tctctaccat gcattgggtt agcaccagga
     3841 gcaaatggag caccagctct tctaccatct ggtgtattac cagttttctt accataaaca
     3901 acatttgaag ttattgtaag tatagattga gtaggataag aatttctata agttttattc
     3961 tttcttattt tattcataaa gctttcaact aactctacag ctatactatc aactctatca
     4021 tcattatttc cgtattttgg ataatctcct tctatttcaa aatcaacagc tattccttgt
     4081 tcatttctaa tagttttaac cttagcatgt tttatagcag ataatgagtc tgcacaaact
     4141 gataatccag ccataccaca agccatagtt ctaaatatat ctctatcatg taatgccatt
     4201 tctaatgctt cataagaata tttatcatgc atataatgta ttacatttaa agtatttaca
     4261 tataatgttg ctaaccaatc agtgaatggt tcaaatttac tcataacttc atcataatct
     4321 aagtactcag aagttatagg ttcaaatctt ggtccaactt gaacaccaga tttctcatct
     4381 acaccaccat ttatagcgta taataaagtt ttagctaagt taactctagc tccaaagaac
     4441 tgcatttgtt taccaattct cattgcagat acacaacaag ctataccata gtcatctccc
     4501 cagtatgctc tcattaaatc atcattttca tactgtactg aacttgtatc tatagatact
     4561 ttagaacaga aatctttgaa tccttgaggt aatttagttg accatagaac tgttaagttt
     4621 ggttctggag aaggccctaa tgtatataga gtatttaaca ttctaaatga gtttttagta
     4681 actaatgttc taccatctat tcccatacct cctatacact cagttaccca agttgggtct
     4741 ccagagaata agttattgta atcaggagtt cttaggaact taaccattct taatttcata
     4801 acaaaatggt ccatatactc ttgtaattct tcttctgtta cgattccagc ttttaaatct
     4861 ctttcaaagt atatatctaa gaaagtagat gttctaccta aagacattgc agcaccattt
     4921 tggtctttaa cagcacctaa ataaccaaag tataaccatt gtacagcttc ttttgagtta
     4981 gttgctggtt ttgatatatc aaatccataa gattcagcca tttttttaag ttcatttaaa
     5041 gctgatattt gctcagttat ttcttctctt aatctgataa cttcctcatc catgcaagaa
     5101 acttctaatg atttcttttg ctcattttta tcttctatta aagcatcaac accatataga
     5161 gcaactcttc tgtagtcacc aattattcta cctctaccat aagcatctgg aagacctgtt
     5221 attataccag attttctagc agctctcatt tctggagtat aaacatcaaa aactccttgg
     5281 ttgtgtgttt ttctgtattt agtaaatata tcttttattt ctgggtctaa ttcatatcca
     5341 aaagcttcgc atgaattttc aaccatcttt ataccaccat atggcatgac agctcttttt
     5401 aaaggggcat ccgtttgtaa cccaactatt gtttctaatt ctttatctaa atatcctgga
     5461 gcatatgcag ctattccaga aacagttttt gtatcaacat ctaaagtacc accattttct
     5521 ctttcttttt taaataggac cattgcttcg tcccataatt ttttagtatt ttcagtagca
     5581 ccagctaaga aagagtcatt tccctcatat ggagaatagt ttaattgtat aaactctcta
     5641 acatttattt ctttagtcca tcttcctgtt ttaaatcctt gccatgcatt cattaagtat
     5701 ttcctcctta gatttaaatg ataaataata ctattaaaat taatttttat atacttaatt
     5761 tattttatat atttttttaa aaatatattt tctaatataa taatatcata ttattcgtat
     5821 cctgtataca taaaaaataa aaaattgtcg ctttaaagta taaaacagat tatatgttaa
     5881 taatgtggtg aaattatacc catatataaa taaaataaaa caaataaata gctaattaag
     5941 gagaaaatat tttttatttt ttgtttagga taaataaatt ttattatgga aaaaagaaaa
     6001 agcatacatc ttcacaaatt gaatatttat ttaatagggg gaggtgatag attgtttatc
     6061 acagtggtat tatttttagt aggtttccta ttaattacta aaggcgcaga tatctttata
     6121 aattgcactg tagagatagg taaaaaaacg aatatatcag aaattatact tggagctact
     6181 atagttagtt ttgcaacaac attacctgaa tttacagtgt ctctacttgc ttctattgat
     6241 ggtcatacta ctatgagttt aggaaatgca gttggttcta taatatgtaa tacaggatta
     6301 gcattagggt tggttgtttt tataagtcca tttaatgtag ataaaaaaat gtttttttct
     6361 aagtctttac tattaatagt atccgttata gttttaattt tattgagttt agatggagtt
     6421 ataacaagag gtgattcatt attacttata ataatattaa tcttttatat ggttaataat
     6481 tatagaagtg tcgttggaaa atctgatact aaaaatagaa atattaaaaa taacactatt
     6541 aaagacatat ctactaaaaa agggaaaaat tatagaggtt tttccgtatt agaaattgta
     6601 aaaatactat tactttttgc tacaggattg ataatgatga taataggttc acaaatcctt
     6661 atagagagtg gagtaataat agctagtttt ttaaatatac ctcaaggtat agtaagttta
     6721 actataatag cacttggaac ttctctgcca gagatagttt cgtcgattac tgctataaga
     6781 aaaaatcatc atgaaatatc agtaggaaat attttgggtg caaacatatt gaatatagta
     6841 tcagtaatag ctgtatcagc aataccaaat aacataccaa tactatctca aaatagacaa
     6901 ttggatatac cattcatgat attattatta ttaatagtaa taataccaac acttaaatct
     6961 aatagactta gtagaataca aggtatattg atgttattca catattttct gtacatatca
     7021 attttgtatt tcatgtatat tatttaacta tactgtttaa aatattaatt gcagaatatc
     7081 ctaaaaaagt atataatgga taggttaaga ttaattgata acggagagga ataatatatg
     7141 aatataacaa agtttgaaga tttaccaata agtgagggaa taaaaaaagc tatagctgaa
     7201 atggggtttg aagaaccatc tcctatacaa gcacagtcta tacctgcgat tttgtcaggt
     7261 aaagacgtta taggacaagc tcaaactggt acgggaaaaa cagctgcttt tagtatccct
     7321 atactagaaa caatagaccc aaataataga agtttacaag ctgtagtgct ttgtccaaca
     7381 agagaactgg caatacaagt atcaacagaa ataagaaagt tagctaagta ttcgcacgga
     7441 ataaagacat tacctatata tggtggtcag ccaatagata gacaaataaa atctctaaag
     7501 agtggtgtgc aagttgtaat aggtacacca ggacgtacaa tagaccatat caatcgtaaa
     7561 acattaaaga tggataatgt aaaaatgatt atattagatg aagcagatga aatgctagat
     7621 atgggattta gagaagatat agaaatgatt ttaagtaaga ttccagaaga aagacaaaca
     7681 actttctttt cagctactat gccaagagga attttggaat taactaagag atatcaaaaa
     7741 gacccagagc atataaaagt tgttagaaaa gaacttacag tttcaaatac aaagcaatac
     7801 tatatagaaa ctaggtcatc aaataaatta gaagtattat gtagattagt agatgtatat
     7861 gaccctaaac tatctgttgt tttctgtaat actaagagaa aagcagacga attggtagga
     7921 gatttacaag caagaggata ttttgcagat gcattacatg gtgacttaaa acagactcaa
     7981 agagatatag taatggataa gtttagaaat ggtacaatag atatacttgt tgctactgat
     8041 gttgcagcta gaggtataga tgttgatgat gttgaatgtg tattcaacta tgacttacca
     8101 caagatgaag agtattacgt tcatagaatt ggtagaactg gtcgtgctgg tagagaaggt
     8161 atgtcattta cttttgtatt tggaaaagaa atgagaaaga tgaaagatat agaaagatat
     8221 actaaatcta aactaataaa gcataatata ccaacaataa ctgatgttga agagaaaaaa
     8281 gtaggtactt tctttgcaca agttaaacaa actatagaag aaggtcactt aactaaacaa
     8341 ttacaatggt tagaaggttt ctgtaatgat gaagattatg caatggttga tatagctgct
     8401 gcacttgtaa aactatctct aggtgaagaa atgaaagaag aaataataga agaaaaacct
     8461 agaagagagc gtggagatag aaaaggtggt actggagcta aagatggtat gattagatta
     8521 tttatcaata ttggtagaaa tcaaagagta caagcaaaag atatagttgg tgccatagct
     8581 ggagaagttg gaataccagg taaagtagta ggtactatag atatatatga taagtatact
     8641 tttgttgaaa tacctaaaaa agatgcaaaa acagtaattg aaaagatgaa agatattaag
     8701 ataaaaggta ataagattaa tatagaaaaa gcaaacaaga agaaaaaata acgtatagta
     8761 tacaaaacta taattagaaa atatataaaa taatatattg gatatttaaa aagcctgatg
     8821 tgttttcaaa acagatagta gataatctgt aagagttaac agcataaggc ttttcttgtt
     8881 ttatatgatg ttataatttt ccagattgtt gtgaaaaatg tatattagtt cctttttttc
     8941 aaatctgatt tgaaaaagag aatattaatt atctatatag tttagattat aataaaaaat
     9001 gtaagataac aaaggggaga aaaagatgtc aatgacttat atggacccta gggttttttt
     9061 gattttagaa atggtgttag aagaagacac aaatactcta gaacaatttt caaatgtttt
     9121 gggtgttagt actaggacta tcagaaacta tataaaccag attaatcgag aattaaaagg
     9181 tggtattgct aaaatagtta aaaatgaaaa aggtacatat agtataagaa ttgaggatga
     9241 aagtaaatta attgagattg taaattttaa tagaggaaag ctatcaaatt ttataaattt
     9301 aaattcacct gatgaaagaa taaattatgt tcttgatatt ttaactatga ccaacaatcc
     9361 aattaccata gatgatttgg ctgaagagat atgtatagga agaacaacat taataaagga
     9421 tttaaaaaaa gcagacagaa ttttagctga atatgattta gaattaaaaa ggaagccaaa
     9481 tataggtatg attttagttg gtgaagaaat ggatataaga ttattaatcc ttgatagatt
     9541 gtatgaaaat tatattgatg tcttggaaaa cgttaatcag ataaatttag ttaaagatta
     9601 tgatattgaa tgtcttagag atgaattaaa aaagttattt aaaaaagaag aactctacat
     9661 aacagaacag gtcttgagag atgttgaaag atatataatc atgtctgtct taagaaatct
     9721 aaatgggtac aaatttgaaa agatagataa gagatttgaa gtgataagat gttcagatga
     9781 atatacttta ggattaaagc tcaaagcttt attggaaaag atatttaata tagttttaaa
     9841 tgaaaaagaa accatatttt tgacaatgcc attaataggt agaaaagctc ctagtacaaa
     9901 attagcactt agcagtataa agataaatga tagcgtaaag gaagttgtag atgaagtaac
     9961 tagtttttta ttagagacct cagggattga ttttaaagaa gataaaaagc ttatagaaaa
    10021 tttggaatat cacttgtatt ttgcacttaa tagaatgaga ttcaacataa gggtaaaaaa
    10081 tcctctttta caagaaataa aggaaagata tacatttcca tatagtgttg caaagatagc
    10141 tgcaatgata attgaggaaa agtttgactt gaaaatatgt gaccacgaaa taggatatat
    10201 agcattacat tttggaagtt attttgaaaa aaattcaaga aaagttctaa atgtaaatag
    10261 ggtagctgtt gtttgtggaa caggtcttgg gactgcacaa cttattagaa taaagatagg
    10321 aaaaatactt ggagaaagtg tagaagtaaa tactttttct gatatagaag ctaaagctaa
    10381 tttagattta ttaaagtatt atgatttagt atttacaaca gtagatttca aaacagactt
    10441 agatgttcca atttttagag taaatgcgtt atttaatgaa gaaagtataa agaaagagat
    10501 agaaaatgca atacttttga aaaatagtat aaatcttaag tctataaaaa agattgaaaa
    10561 aaatatccct tttataggta aattaattga agaggataag ttctttatat tgaataagag
    10621 tacgtttatg gaaaatttag aagatatgtt agaaaaatta gttcataaag gaattattga
    10681 tatagagttc aaaaaaaagg tattaaaaag agaaaagaaa tcttcaacag cattagataa
    10741 ttatatagca ttgcctcact cagttaatac taatggggaa aatctttatc tggcatttgg
    10801 aatattagaa aaaccagtga tttgggatac aaaggaaatt agaataataa tattgatgat
    10861 aattccagat aagaatgtag atagcacaga cctgataata aaaagttatg aagaagtact
    10921 tgaagtaggg agagataaaa agatggtaga agaacttgct aaggtaaaat catttgatga
    10981 attttataaa ataattacca agaggaggta ataatatgga cccagtaatt tttattatag
    11041 catcacttat tacagtgtat ttattaaata tttttcttgg atacttacag cttaaagatt
    11101 ttaataaaaa ttatattgaa ctaaaaagaa aaggaagagt ggcaattggc agaaaaaaag
    11161 gaagaatttc atcaggtaca attgtgttga tacttataaa tgatgatggt gtgattgtcg
    11221 agactagaaa aatgcaaggt gttacagtat tggcaagagt aaagtcattt gaaggtttgg
    11281 taggaaagag cttagagtat atagaaaaat cagatttaac tgtttacaat aaactactta
    11341 aaaaagcaat actagatgca gtcaaacagt acataacatt tgagaaaaat aaatgtgaag
    11401 atacaaataa tctgagtact gatgaagagt gtaggatgga agtagtttaa aaaaatatat
    11461 ttataaggag gagttaatat gacgcaattt tttcaagtat tagcaagtgg tgcagatagt
    11521 tttatgaatt tatttagggc aggtgggcaa caatttgtag gttttgtaac agatatagta
    11581 ccacttttag tagcattatt agttacaatg aatgcaatta tcaactttat tggtacagat
    11641 agagtcgaga gacttgcaaa aaaatgttct gcaaatatat ttactagata tttgatatta
    11701 ccagtccttg gtacatttgt atttgcaaac ccaatgacac tatcattagg aagattcttg
    11761 ccagaaaagt ttaaacctag ttactttgca gcagcaagct tttcatgcca tacaatgaat
    11821 ggattattcc cacatatcaa tccaggtgaa ctatttatat atctaggaat tgcaaatggt
    11881 ataacaactt tgggattctc aacagcagat ttagcagtta gatatttgtt agtgggtata
    11941 gttgcaaact ttattaaagg tgtagttact gactatacaa caaaatttgt agagaaacaa
    12001 caaggaatag cattaaattc agatataaag attggataat tactttaagg tataaataaa
    12061 tttttcaaat aaaaagatag tgtatagagg aggataaatt atatggcatt taacaaagtt
    12121 aaaatagtaa aaggttctgg aggatggggt ggaccattga taatagaacc aacagaaaag
    12181 aagaataagg tagtatatgt gactggggga gcaaagccag aaactgcagt aaaaatagct
    12241 gaacttacag gatgtgagtt aatagatgga ttttcacagg gtgttaaaga tgatgaaatc
    12301 gcatgtgtaa taataaattg tggtggaaca ttgagatgtg gtatatatcc tcaaaagaaa
    12361 ataccgacta taaatataat gaaaacagga aaaagtggac cattagcaat gtttataaaa
    12421 gaagatatat atgtttcagg ggtaaaacca acaaatgtag agcagttata attgaggtag
    12481 taggagggaa gaaatatggc ttatgatact aaaaagaaga taagtgagca gagtcaagga
    12541 attgttgcaa aaataggtat agcactagga aatgtgacaa gtactctttt tcaagctggt
    12601 agggatacta tagatacatt aatacatact atactgcctt ttatggcttt cgttgcagtc
    12661 ctaataggta taataaatca atcgggtata ggaaattggg tagccaattt acttactcca
    12721 ttaatcggaa atgttggagg tttagttgtc ttatcaataa tatgttcatt tccgttgttg
    12781 tctccgtttc ttggaccagg agctgttata gctcagatag tgggggtatt ggttggtaca
    12841 gaaataggaa aaggaaatat tccaccaagt ttagcattac cagcattgtt tgcaataaac
    12901 tcacaagcag cttgtgactt tataccagta ggtcttggtc ttgcagaagc tgaacctgat
    12961 acagttcaag taggagttcc gtcagtttta tattctagat ttttaacagg tgcaccaact
    13021 gttctattag catggttagc aagctttggg ttatataaat agaagaatac tttataaata
    13081 attagagaga ggttcagttg atatggaatt aatatacagt actcaaataa ataaaatagg
    13141 agataatgca aatgaatttt tacaacataa tatgtttgta actttcaaag ataatgctcc
    13201 tcaagagtta gaaaattact gttatataca ttctgaaaat aatttagtaa aagaaatact
    13261 tacggatgat gtgctatcaa taaataatgt tgattacaaa attacttctg ttggtgagtt
    13321 agtaaaccaa aatctttcag aattaggtca tataacattt aagtttacag gtgaaaaaga
    13381 tggagcaatt ggaggaacct tatatcttga agaaaaagaa atagcaccta ttggcgtagg
    13441 tactatacta aaaataatta gaaaataaaa tttaaaacga atctaatcat attataagta
    13501 aattatagat aaaagtcaat ttaactaatc aagttaactt ttaatgtgat aaaaaagggg
    13561 aaatgaaaat gagaaaagtg gctttagtcg ttggaggagg aagaagttta ggtgaatatt
    13621 tatctaaaca tttagctaaa gtaggttatg atgttgctat tgcagattta aatgaagaca
    13681 acgcaaaaaa agtagctgaa gaaataaaaa ctgaatttgg ttgtaaatct gtaggatata
    13741 gttgtaatgc catagatgaa agacaggtta taagtactgt caaagctatt gaaaatgatt
    13801 ttgatagaat agatttatta gtgtacaatg caggagtggc tgtaagtaaa aaaataactg
    13861 attttggata caaagatttt aaatttattg tagacgtaaa tttaaatggg tactttctat
    13921 ttgcaagaga agtatctaaa gtcatgatag caaaaggtat tgagggtaga ataatacaga
    13981 taaattcaaa atcaggtaga gtgggaagca agcataattc aggatattca gcaggtaaat
    14041 ttgctggagt aggacttact caaagtttag cgctagattt ggcagaacat aagataactg
    14101 taaatgcact gatgctcggt aatttacttg attctgatat gtttgaaagt ttaattcctc
    14161 aatatgcagt aaaattggga atacctgaat cagaagtcaa gcaagtttat atagataaag
    14221 tacctttaaa aagagggtgt agatttatag atgttgcaaa tatgcttaca ttttatgctt
    14281 caaaggaagc tgaatactgc actggtcaat ctataaatat tactggtgga caagttatgt
    14341 aaggttttta tggagtgtct caagatgaac tctttagttc atgaggcact tttttgtata
    14401 aaattaaata tattttcagc atttaaacaa tgaaaaagag gtttatcttt attttgagat
    14461 agcctcattt ttgtaattaa aaaacatagt gtttaagtaa acatataaga ttgttagttt
    14521 tttaaaagag aattttttaa taatattata tttatgaagt atttttaaac ttaatttgat
    14581 aaaatatagc tatgttttat atactgtagt tttgaacata gaaagtttaa taaattatag
    14641 tatatatgtt aatttttaat aaggtaagaa agtagaaaga aggatatttt atggatataa
    14701 taaaaaaatt aagtcatggg tgtacattag attgtcatga ctgttgtaaa ttcaatgttt
    14761 atactaaagg aaataatgta gtaaaaattg aaggagataa aaatcatcct tatacaaaag
    14821 gttttatatg caaaaaaggg atggcccatt tagatagatt aaatcataaa gatagaataa
    14881 agactcctat gcttaaagtt gatggagtat ggaaagagat ttcttttgat aaagctatag
    14941 aaataatggc agaaaagctt acatactata aagaaaagta tacatcaaag tctgtcatgc
    15001 attatgacca atatggaagt gggtctgtgc taaaatacat aggagatata ttttttaatt
    15061 tttatggagg tgttagcaga cataagggtg gtccctgttg gagtgcaggg atgcatgctc
    15121 aaaaatatga ttttggagta gctaagagtc atgctataga agatatgtta aatagtaaaa
    15181 gtatatttgt ttggggaaaa aatccagcat atactacaat tcatactatg caaataatta
    15241 aaaaagctaa agaaaaaggg ataaagatag tagtgattga ccccatatat acaaaaacag
    15301 ctcaaatagc agataagtat gtacaagtaa atcctggaac agatggagct ttagctatag
    15361 ctatggcaaa aattatagtg gaagataaat tatatgatga agaatatata aactcgtatg
    15421 ttattggttt tgaagaatat aaaaaatatt tatcttcatt ggaattaagt tttttaattg
    15481 atgaatgtgg tgttaaagag aatgatataa gagagttagt ggatttatat actaacaaat
    15541 attcaagtat aaatgttgga tatgggcttc aaaaatataa aaatggtgga aacacaataa
    15601 gagcaataga tgctttaggt gctataactg gtcaaatagg atttagtggt ggaggggtaa
    15661 actatgctaa taaagtttat ccgagtgtta taaattctga cccttacaat agccaatcat
    15721 atggtgaaga tagggagttt tatgtaagta atatcagtaa atttatagaa gaatctttaa
    15781 aaaatacatc aaataaagta aattatgcat cagatgaact tgatatggca ttacataaag
    15841 taaattacat atcagatgaa cttgatacaa catcaaataa agtaaattat gtatcagatg
    15901 aacttgatat ggcattacat aaagtaaatt atgtgtcaga tgaacttgat acaacatcaa
    15961 ataaagtaaa ttatgtatca gatgaacttg atatggcatt acataaagta aatggtgtat
    16021 cagatgaact tgatatggca ttacataaag taaattgtgt atcagatgaa cttgatatga
    16081 catcaaataa agtaaattat gtatcagatg aacttgatat gacatcaaat aaagtaaatt
    16141 atgtattaga tgaacttgat ataacatcaa ataaaacaga ttatatatca aatgaattgt
    16201 ataatttatc taataagtca atcaaagata atattcctat aaagatggct gtgataacta
    16261 aaagtaatat gctgaatcaa ctgccagatt tagtagaatt ggagagggtg ttttcaaaaa
    16321 ttgaatttaa agtatgtttt gatatgttta taactgatac agttacttta tgtgacatgt
    16381 ttataccgtg tacaaataca ctggagagtg aagatataat ttatagctca atgaccaatc
    16441 catatataac ctataatgaa agagctgtaa agccagcaca taaattgatg gatgaatatt
    16501 acttctttat ggagctagct aaaaagatgg gattaaatga ttatcctttt gtagaaaaga
    16561 gaacttattt agaaaaagtg attgaaccac ttaagcgttt tgacaaaaat ctggatatag
    16621 aaaaacttaa aaacaattac tttactatac ataaccctgt tgcttgggaa gataagaagt
    16681 ttgagactcc atctggaaaa tacgagcttt attcagaaag tataaaaaac ttgggaataa
    16741 gtccaacacc tgtatatata agcaataaat ataaagaaat agaagataaa aatatttctt
    16801 ttagattatt aactaatcat catgcagata ctttatttag tcagcatttt atggacaaaa
    16861 aatctatagc tcaagcatat ataaatcaga gaatggctaa aaaagtaggt attgaggaga
    16921 aagatattgt gatattgagg tcaaaaaaag caaaaataaa tgtacaaata aatatagatg
    16981 atggagtagg taattatata gtaaaaatgt atgttggttg gtggaagaaa catggaaatc
    17041 ctaatagttt aacagacact ggtatatcag attttggggg tcaggtaaca tataatgaaa
    17101 gtatggtaga aataatacga caaaactagt taaatttcat tgaaggaata aaaatataat
    17161 tatagaattg attaaaagtt actattttta caaaactatt gaaggaggga tttttgatgg
    17221 taaattattc cttagaacat aagaatctat acatagaatt tatgtgctca gaactagacc
    17281 accatgtagc caatgaaata agggaagaaa tagataactt attaagtgta aatcaagtaa
    17341 aaaatgttgt atttaatttt gaaaatatta attttatgga ttcttcaggt ataggcgtca
    17401 tcataggtag atataaaaag atttctaatg aagggggacg agtttcagta attaatataa
    17461 gttcacgagt taagaagatt tttgatttat ctgggttaaa taaaattatt ggcatttatg
    17521 atacgtatga agaagctcta agttctttgt agggaggagt atgtgatgaa taatattatg
    17581 gaagttaaat tttcggcaat atctgaaaat gaaagtttag caagagttat agtagcatcc
    17641 tttgcagcta agttagaccc aacagtagat gagttagtag atataaaaac tgctgtctct
    17701 gaggctgtta cgaatgctat aatacatgga tatgaagaag acagttctaa gtttgttttc
    17761 ttaagatgtg agatagaagg aaatactata aagattatag ttgaagatga agggtatgga
    17821 atagaaaatg tggagaaggc aatggaacca ctttatacat caaaaccaga acttgataga
    17881 tcaggaatgg gattcacagt aatgaaaagc tttatggacg atgtagaggt aagttctgta
    17941 aaagataatg ggactagaat tgaaatgact aaaaaaataa atgtgcctaa ataaagtcca
    18001 aaagtgaggt tttattatgg aagtaactgt tgccagagaa gaaaaaaaac ctcttcttag
    18061 tcatgaagag actctagaac ttattgaaaa agttcaaaat ggagatgaag aggcaaaaga
    18121 aatattaata tcaagcaatt taggtctagt taggagcgtt gtatctagat ttttaaatat
    18181 aggatatgat agagaagatt tatttcagtt aggttctata ggcttaataa aatcaatata
    18241 taaatttgac ccaaaattta atgtaaaatt ctctacttat gcagtaccaa tgattttagg
    18301 tgaaataaag agatacttaa gagatgatgg tatgataaaa gtatcaaggt cgttaaagca
    18361 aatagctgta aaggcaaaga tggaaagtga agctttaact aaaaaattag gtagagagcc
    18421 gagtatagaa gaattagcta aagcaataga tgtagaaaaa gaagatttag taatggctat
    18481 ggaagctaat ttcaatgttg agtatttgca aggagttatt catgaagagg aaggttctcc
    18541 aatatgcctt attgataaaa taagcatgaa aggagaaagt gaagaagaaa aagtagtgga
    18601 taacatattg ttaaaagaca tacttggaag actggataag agagagcgtc aaataatagt
    18661 tctcagatac tttgaagaca tgactcaaag tgaaatagga gagatgctca atatatcaca
    18721 agttcaagta tctaggatag agaagaaagt attgtctaag ttaaaagaat atatatcgtg
    18781 ataatactta tgatatgtag aataacaaaa tataataa
//
LOCUS       DAMJWU010000068        18699 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMJWU010000068 DAMJWU010000000
VERSION     DAMJWU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.069"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            113..667
                     /gene="tcdR"
                     /locus_tag="PC460_002526"
     CDS             113..667
                     /gene="tcdR"
                     /locus_tag="PC460_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF2601406.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            985..8085
                     /gene="tcdB"
                     /locus_tag="PC460_002527"
                     /gene_synonym="toxB"
     CDS             985..8085
                     /gene="tcdB"
                     /locus_tag="PC460_002527"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF2601407.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8207..8707
                     /gene="tcdE"
                     /locus_tag="PC460_002528"
     CDS             8207..8707
                     /gene="tcdE"
                     /locus_tag="PC460_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF2601408.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            9036..9218
                     /locus_tag="PC460_002529"
     CDS             9036..9218
                     /locus_tag="PC460_002529"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601409.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9435..17567
                     /gene="tcdA"
                     /locus_tag="PC460_002530"
                     /gene_synonym="toxA"
     CDS             9435..17567
                     /gene="tcdA"
                     /locus_tag="PC460_002530"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF2601410.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17902..18600)
                     /gene="tcdC"
                     /locus_tag="PC460_002531"
     CDS             complement(17902..18600)
                     /gene="tcdC"
                     /locus_tag="PC460_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HDF2601411.1"
                     /translation="MFSKKNDGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT         7388 a         2065 c         2899 g         6347 t
ORIGIN      
        1 atataaaatt taatttattt gccgattata taattataat gactgattta attccgatgt
       61 tgtcaaaatt ttcaaataaa tcatcattat aaatataaga gaggatgatt ttatgcaaaa
      121 gtctttttat gaattaattg ttttagcaag aaataactca gtagatgatt tgcaagaaat
      181 tttatttatg tttaagccat tagtaaaaaa gcttagtaga gttttacatt atgaagaggg
      241 agaaacagat ttaataatat tttttattga attaataaaa aatattaaat taagtagctt
      301 ttcagaaaaa agcgatgcta ttatagtcaa atatattcat aaatcattac tgaataagac
      361 ttttgagttg tctagaagat attctaaaat gaagtttaat tttgtagaat ttgatgaaaa
      421 tatcttaaat atgaaaaata attatcaaag taagtctatt tttgaggaag atatttgttt
      481 tttcggatat attttgaaag aattatctgg tattcaaaga aaagttattt tttataaata
      541 tttaaaagga tattctgata gagaaatatc agtgaaatta aaaatatcta gacaagctgt
      601 taataaggct aaaaatagag catttaaaaa aataaaaaaa gactatgaaa attattttaa
      661 cttgtaatta atgagcttaa agaaatattt acaatagaaa tcaaatttta gaattaactt
      721 tattgtaaaa tcaataactt aatctaagaa tatcttaatt tttatatttt atatagaaca
      781 aagtttacat atttatttca gacaacgtct ttattcaatc gaagagcaaa ttaatcaact
      841 gagtgtcttc aatttaaaat gttaggaagt gaatgtatat gaaaacctaa gtagatatta
      901 gtatatttta taaatagaaa ggaggatata taaaagagtt ttatcattta gatgtaaaaa
      961 tattatagta aaggagaaaa ttttatgagt ttagttaata gaaaacagtt agaaaaaatg
     1021 gcaaatgtaa gatttcgtac tcaagaagat gaatatgttg caatattgga tgctttagaa
     1081 gaatatcata atatgtcaga gaatactgta gtcgaaaaat atttaaaatt aaaagatata
     1141 aatagtttaa cagatattta tatagataca tataaaaaat ctggtagaaa taaagcctta
     1201 aaaaaattta aggaatatct agttacagaa gtattagagc taaagaataa taatttaact
     1261 ccagttgaga aaaatttaca ttttgtttgg attggaggtc aaataaatga cactgctatt
     1321 aattatataa atcaatggaa agatgtaaat agtgattata atgttaatgt tttttatgat
     1381 agtaatgcat ttttgataaa cacattgaaa aaaactgtag tagaatcagc aataaatgat
     1441 acacttgaat catttagaga aaacttaaat gaccctagat ttgactataa taaattcttc
     1501 agaaaacgta tggaaataat ttatgataaa cagaaaaatt tcataaacta ctataaagct
     1561 caaagagaag aaaatcctga acttataatt gatgatattg taaagacata tctttcaaat
     1621 gagtattcaa aggagataga tgaacttaat acctatattg aagaatcctt aaataaaatt
     1681 acacagaata gtggaaatga tgttagaaac tttgaagaat ttaaaaatgg agagtcattc
     1741 aacttatatg aacaagagtt ggtagaaagg tggaatttag ctgctgcttc tgacatatta
     1801 agaatatctg cattaaaaga aattggtggt atgtatttag atgttgatat gttaccagga
     1861 atacaaccag acttatttga gtctatagag aaacctagtt cagtaacagt ggatttttgg
     1921 gaaatgacaa agttagaagc tataatgaaa tacaaagaat atataccaga atatacctca
     1981 gaacattttg acatgttaga cgaagaagtt caaagtagtt ttgaatctgt tctagcttct
     2041 aagtcagata aatcagaaat attctcatca cttggtgata tggaggcatc accactagaa
     2101 gttaaaattg catttaatag taagggtatt ataaatcaag ggctaatttc tgtgaaagac
     2161 tcatattgta gcaatttaat agtaaaacaa atcgagaata gatataaaat attgaataat
     2221 agtttaaatc cagctattag cgaggataat gattttaata ctacaacgaa tacctttatt
     2281 gatagtataa tggctgaagc taatgcagat aatggtagat ttatgatgga actaggaaag
     2341 tatttaagag ttggtttctt cccagatgtt aaaactacta ttaacttaag tggccctgaa
     2401 gcatatgcgg cagcttatca agatttatta atgtttaaag aaggcagtat gaatatccat
     2461 ttgatagaag ctgatttaag aaactttgaa atctctaaaa ctaatatttc tcaatcaact
     2521 gaacaagaaa tggctagctt atggtcattt gacgatgcaa gagctaaagc tcaatttgaa
     2581 gaatataaaa ggaattattt tgaaggttct cttggtgaag atgataatct tgatttttct
     2641 caaaatatag tagttgacaa ggagtatctt ttagaaaaaa tatcttcatt agcaagaagt
     2701 tcagagagag gatatataca ctatattgtt cagttacaag gagataaaat tagttatgaa
     2761 gcagcatgta acttatttgc aaagactcct tatgatagtg tactatttca gaaaaatata
     2821 gaagattcag aaattgcata ttattataat cctggagatg gtgaaataca agaaatagac
     2881 aagtataaaa ttccaagtat aatttctgat agacctaaga ttaaattaac atttattggt
     2941 catggtaaag atgaatttaa tactgatata tttgcaggtt ttgatgtaga ttcattatcc
     3001 acagaaatag aagcagcaat agatttagct aaagaggata tttctcctaa gtcaatagaa
     3061 ataaatttat taggatgtaa tatgtttagc tactctatca acgtagagga gacttatcct
     3121 ggaaaattat tacttaaagt taaagataaa atatcagaat taatgccatc tataagtcaa
     3181 gactctatta tagtaagtgc aaatcaatat gaagttagaa taaatagtga aggaagaaga
     3241 gaattattgg atcattctgg tgaatggata aataaagaag aaagtattat aaaggatatt
     3301 tcatcaaaag aatatatatc atttaatcct aaagaaaata aaattacagt aaaatctaaa
     3361 aatttacctg agctatctac attattacaa gaaattagaa ataattctaa ttcaagtgat
     3421 attgaactag aagaaaaagt aatgttaaca gaatgtgaga taaatgttat ttcaaatata
     3481 gatacgcaaa ttgttgagga aaggattgaa gaagctaaga atttaacttc tgactctatt
     3541 aattatataa aagatgaatt taaactaata gaatctattt ctgatgcact atgtgactta
     3601 aaacaacaga atgaattaga agattctcat tttatatctt ttgaggacat atcagagact
     3661 gatgagggat ttagtataag atttattaat aaagaaactg gagaatctat atttgtagaa
     3721 actgaaaaaa caatattctc tgaatatgct aatcatataa ctgaagagat ttctaagata
     3781 aaaggtacta tatttgatac tgtaaatggt aagttagtaa aaaaagtaaa tttagatact
     3841 acacacgaag taaatacttt aaatgctgca ttttttatac aatcattaat agaatataat
     3901 agttctaaag aatctcttag taatttaagt gtagcaatga aagtccaagt ttacgctcaa
     3961 ttatttagta ctggtttaaa tactattaca gatgcagcca aagttgttga attagtatca
     4021 actgcattag atgaaactat agacttactt cctacattat ctgaaggatt acctataatt
     4081 gcaactatta tagatggtgt aagtttaggt gcagcaatca aagagctaag tgaaacgagt
     4141 gacccattat taagacaaga aatagaagct aagataggta taatggcagt aaatttaaca
     4201 acagctacaa ctgcaatcat tacttcatct ttgggggtag ctagtggatt tagtatactt
     4261 ttagttcctt tagcaggaat ttcagcaggt ataccaagct tagtaaacaa tgaacttgta
     4321 cttcgagata aggcaacaaa ggttgtagat tattttaaac atgtttcatt agttgaaact
     4381 gaaggagtat ttactttatt agatgataaa ataatgatgc cacaagatga tttagtgata
     4441 tcagaaatag attttaataa taattcaata gttttaggta aatgtgaaat ctggagaatg
     4501 gaaggtggtt caggtcatac tgtaactgat gatatagatc acttcttttc agcaccatca
     4561 ataacatata gagagccaca cttatctata tatgacgtat tggaagtaca aaaagaagaa
     4621 cttgatttgt caaaagattt aatggtatta cctaatgctc caaatagagt atttgcttgg
     4681 gaaacaggat ggacaccagg tttaagaagc ttagaaaatg atggcacaaa actgttagac
     4741 cgtataagag ataactatga aggtgagttt tattggagat attttgcttt tatagctgat
     4801 gctttaataa caacattaaa accaagatat gaagatacta atataagaat aaatttagat
     4861 agtaatacta gaagttttat agttccaata ataactacag aatatataag agaaaaatta
     4921 tcatattctt tctatggttc aggaggaact tatgcattgt ctctttctca atataatatg
     4981 ggtataaata tagaattaag tgaaagtgat gtttggatta tagatgttga taatgttgtg
     5041 agagatgtaa ctatagaatc tgataaaatt aaaaaaggtg atttaataga aggtatttta
     5101 tctacactaa gtattgaaga gaataaaatt atcttaaata gccatgagat taatttttct
     5161 ggtgaggtaa atggaagtaa tggatttgtt tctttaacat tttcaatttt agaaggaata
     5221 aatgcaatta tagaagttga tttattatct aaatcatata aattacttat ttctggcgaa
     5281 ttaaaaatat tgatgttaaa ttcaaatcat attcaacaga aaatagatta tataggattc
     5341 aatagcgaat tacagaaaaa tataccatat agctttgtag atagtgaagg aaaagagaat
     5401 ggttttatta atggttcaac aaaagaaggt ttatttgtat ctgaattacc tgatgtagtt
     5461 cttataagta aggtttatat ggatgatagt aagccttcat ttggatatta tagtaataat
     5521 ttgaaagatg tcaaagttat aactaaagat aatgttaata tattaacagg ttattatctt
     5581 aaggatgata taaaaatctc tctttctttg actctacaag atgaaaaaac tataaagtta
     5641 aatagtgtgc atttagatga aagtggagta gctgagattt tgaagttcat gaatagaaaa
     5701 ggtaatacaa atacttcaga ttctttaatg agctttttag aaagtatgaa tataaaaagt
     5761 attttcgtta atttcttaca atctaatatt aagtttatat tagatgctaa ttttataata
     5821 agtggtacta cttctattgg ccaatttgag tttatttgtg atgaaaatga taatatacaa
     5881 ccatatttca ttaagtttaa tacactagaa actaattata ctttatatgt aggaaataga
     5941 caaaatatga tagtggaacc aaattatgat ttagatgatt ctggagatat atcttcaact
     6001 gttatcaatt tctctcaaaa gtatctttat ggaatagaca gttgtgttaa taaagttgta
     6061 atttcaccaa atatttatac agatgaaata aatataacgc ctgtatatga aacaaataat
     6121 acttatccag aagttattgt attagatgca aattatataa atgaaaaaat aaatgttaat
     6181 atcaatgatc tatctatacg atatgtatgg agtaatgatg gtaatgattt tattcttatg
     6241 tcaactagtg aagaaaataa ggtgtcacaa gttaaaataa gattcgttaa tgtttttaaa
     6301 gataagactt tggcaaataa gctatctttt aactttagtg ataaacaaga tgtacctgta
     6361 agtgaaataa tcttatcatt tacaccttca tattatgagg atggattgat tggctatgat
     6421 ttgggtctag tttctttata taatgagaaa ttttatatta ataactttgg aatgatggta
     6481 tctggattaa tatatattaa tgattcatta tattatttta aaccaccagt aaataatttg
     6541 ataactggat ttgtgactgt aggcgatgat aaatactact ttaatccaat taatggtgga
     6601 gctgcttcaa ttggagagac aataattgat gacaaaaatt attatttcaa ccaaagtgga
     6661 gtgttacaaa caggtgtatt tagtacagaa gatggattta aatattttgc cccagctaat
     6721 acacttgatg aaaacctaga aggagaagca attgatttta ctggaaaatt aattattgac
     6781 gaaaatattt attattttga tgataattat agaggagctg tagaatggaa agaattagat
     6841 ggtgaaatgc actattttag cccagaaaca ggtaaagctt ttaaaggtct aaatcaaata
     6901 ggtgattata aatactattt caattctgat ggagttatgc aaaaaggatt tgttagtata
     6961 aatgataata aacactattt tgatgattct ggtgttatga aagtaggtta cactgaaata
     7021 gatggcaagc atttctactt tgctgaaaac ggagaaatgc aaataggagt atttaataca
     7081 gaagatggat ttaaatattt tgctcatcat aatgaagatt taggaaatga agaaggtgaa
     7141 gaaatctcat attctggtat attaaatttc aataataaaa tttactattt tgatgattca
     7201 tttacagctg tagttggatg gaaagattta gaggatggtt caaagtatta ttttgatgaa
     7261 gatacagcag aagcatatat aggtttgtca ttaataaatg atggtcaata ttattttaat
     7321 gatgatggaa ttatgcaagt tggatttgtc actataaatg ataaagtctt ctacttctct
     7381 gactctggaa ttatagaatc tggagtacaa aacatagatg acaattattt ctatatagat
     7441 gataatggta tagttcaaat tggtgtattt gatacttcag atggatataa atattttgca
     7501 cctgctaata ctgtaaatga taatatttac ggacaagcag ttgaatatag tggtttagtt
     7561 agagttgggg aagatgtata ttattttgga gaaacatata caattgagac tggatggata
     7621 tatgatatgg aaaatgaaag tgataaatat tatttcaatc cagaaactaa aaaagcatgc
     7681 aaaggtatta atttaattga tgatataaaa tattattttg atgagaaggg cataatgaga
     7741 acgggtctta tatcatttga aaataataat tattacttta atgagaatgg tgaaatgcaa
     7801 tttggttata taaatataga agataagatg ttctattttg gtgaagatgg tgtcatgcag
     7861 attggagtat ttaatacacc agatggattt aaatactttg cacatcaaaa tactttggat
     7921 gagaattttg agggagaatc aataaactat actggttggt tagatttaga tgaaaagaga
     7981 tattatttta cagatgaata tattgcagca actggttcag ttattattga tggtgaggag
     8041 tattattttg atcctgatac agctcaatta gtgattagtg aatagataaa aatatgttaa
     8101 atatatcctc ttatacttaa atatataaaa ataaacaaaa tgatacacta cataaagtgt
     8161 tctatctaat atgaagattt accaataaaa aggtggacta tgatgaatgc acagtagttc
     8221 acctttttat atttctaatg gtaacaaaat atttttttat ataaacctag gaggcgttat
     8281 gaatatgaca atatcttttt tatcagagca tatatttata aagttagtaa ttttaactat
     8341 atcatttgat acattattag gatgtttaag tgcaataaaa agtcgtaaat ttaattctag
     8401 ttttggaata gatggaggaa tcagaaaagt agcaatgata gcatgtatat tttttttatc
     8461 agtagttgac attcttacaa agtttaactt tttatttatg ttaccacaag attgtatcaa
     8521 ttttttaaga ctaaaacatc ttggaatatc tgaatttttc tctattttat ttattttata
     8581 tgaaagtgta agtatattaa aaaatatgtg cttatgtgga ttaccagtac ctaagagatt
     8641 aaaggaaaaa atagcaattt tactagatgc aatgacagat gaaatgaatg ctaaggatga
     8701 aaagtaagta atggtagata taataaagat attaacaaat aaaaagtgtt atccaaataa
     8761 gaatagctga aagttatcat aattcatgaa actaataatg aaaacgaggg agcagatgcc
     8821 aagagacaca caagtattaa atacatataa tttcgaagca agtgttcatt actatataga
     8881 tgacaaggta gtatatcaaa cattggttca caaagatggt gcatggtcag ttggtaaaat
     8941 ctattaagct acattagtta cagatatcac aaactataat agttaaacat agaaatatgt
     9001 gtaaattgtg atggaaatta ttcaaaaaca caaaaatacg tgatgaagga caaaatgata
     9061 tagaaaataa gtatcaaacc ttaataaatg atttaattga tagtttaaaa gttataggaa
     9121 aaatatataa agaaataaaa acattaaaaa aatataagat atgtttacaa attactatca
     9181 gacaatctcc ttatctaata gaagagtcaa ttaactaatt gagtatcttt aaattgaaat
     9241 gttaggaagt gatttaaata tgaaaactta aattataaaa aatcaatatt aatttatttt
     9301 taaaaaatag aaaggagtgt ataagattta ttttcaaagt ttaaaaacaa gaaaatcaat
     9361 ttaaatttca gaaggaataa atgtggttat agaagtggat ttattatcaa aaataataat
     9421 actaggaggt ttttatgtct ttaatatcta aagaagagtt aataaaactc gcatatagca
     9481 ttagaccaag agaaaatgag tataaaacta tactaactaa tttagacgaa tataataagt
     9541 taactacaaa caataatgaa aataaatatt tacaattaaa aaaactaaat gaatcaattg
     9601 atgtttttat gaataaatat aaaacttcaa gcagaaatag agcactctct aatctaaaaa
     9661 aagatatatt aaaagaagta attcttatta aaaattccaa tacaagccct gtagaaaaaa
     9721 atttacattt tgtatggata ggtggagaag tcagtgatat tgctcttgaa tacataaaac
     9781 aatgggctga tattaatgca gaatataata ttaaactgtg gtatgatagt gaagcattct
     9841 tagtaaatac actaaaaaag gctatagttg aatcttctac cactgaagca ttacagctac
     9901 tagaggaaga gattcaaaat cctcaatttg ataatatgaa attttacaaa aaaaggatgg
     9961 aatttatata tgatagacaa aaaaggttta taaattatta taaatctcaa atcaataaac
    10021 ctacagtacc tacaatagat gatattataa agtctcatct agtatctgaa tataatagag
    10081 atgaaactgt attagaatca tatagaacaa attctttgag aaaaataaat agtaatcatg
    10141 gtatagatat cagggctaat agtttgttta cagaacaaga gttattaaat atttatagtc
    10201 aggagttgtt aaatcgtgga aatttagctg cagcatctga catagtaaga ttattagccc
    10261 taaaaaattt tggcggagta tatttagatg ttgatatgct tccaggtatt cactctgatt
    10321 tatttaaaac aatatctaga cctagctcta ttggactaga ccgttgggaa atgataaaat
    10381 tagaggctat tatgaagtat aaaaaatata taaataatta tacatcagaa aactttgata
    10441 aacttgatca acaattaaaa gataatttta aactcattat agaaagtaaa agtgaaaaat
    10501 ctgagatatt ttctaaatta gaaaatttaa atgtatctga tcttgaaatt aaaatagctt
    10561 tcgctttagg cagtgttata aatcaagcct tgatatcaaa acaaggttca tatcttacta
    10621 acctagtaat agaacaagta aaaaatagat atcaattttt aaaccaacac cttaacccag
    10681 ccatagagtc tgataataac ttcacagata ctactaaaat ttttcatgat tcattattta
    10741 attcagctac cgcagaaaac tctatgtttt taacaaaaat agcaccatac ttacaagtag
    10801 gttttatgcc agaagctcgc tccacaataa gtttaagtgg tccaggagct tatgcgtcag
    10861 cttactatga tttcataaat ttacaagaaa atactataga aaaaacttta aaagcatcag
    10921 atttaataga atttaaattc ccagaaaata atctatctca attgacagaa caagaaataa
    10981 atagtctatg gagctttgat caagcaagtg caaaatatca atttgagaaa tatgtaagag
    11041 attatactgg tggatctctt tctgaagaca atggggtaga ctttaataaa aatactgccc
    11101 tcgacaaaaa ctatttatta aataataaaa ttccatcaaa caatgtagaa gaagctggaa
    11161 gtaaaaatta tgttcattat atcatacagt tacaaggaga tgatataagt tatgaagcaa
    11221 catgcaattt attttctaaa aatcctaaaa atagtattat tatacaacga aatatgaatg
    11281 aaagtgcaaa aagctacttt ttaagtgatg atggagaatc tattttagaa ttaaataaat
    11341 ataggatacc tgaaagatta aaaaataagg aaaaagtaaa agtaaccttt attggacatg
    11401 gtaaagatga attcaacaca agcgaatttg ctagattaag tgtagattca ctttccaatg
    11461 agataagttc atttttagat accataaaat tagatatatc acctaaaaat gtagaagtaa
    11521 acttacttgg atgtaatatg tttagttatg attttaatgt tgaagaaact tatcctggga
    11581 agttgctatt aagtattatg gacaaaatta cttccacttt acctgatgta aataaaaatt
    11641 ctattactat aggagcaaat caatatgaag taagaattaa tagtgaggga agaaaagaac
    11701 ttctggctca ctcaggtaaa tggataaata aagaagaagc tattatgagc gatttatcta
    11761 gtaaagaata catttttttt gattctatag ataataagct aaaagcaaag tccaagaata
    11821 ttccaggatt agcatcaata tcagaagata taaaaacatt attacttgat gcaagtgtta
    11881 gtcctgatac aaaatttatt ttaaataatc ttaagcttaa tattgaatct tctattggtg
    11941 attacattta ttatgaaaaa ttagagcctg ttaaaaatat aattcacaat tctatagatg
    12001 atttaataga tgagttcaat ctacttgaaa atgtatctga tgaattatat gaattaaaaa
    12061 aattaaataa tctagatgag aagtatttaa tatcttttga agatatctca aaaaataatt
    12121 caacttactc tgtaagattt attaacaaaa gtaatggtga gtcagtttat gtagaaacag
    12181 aaaaagaaat tttttcaaaa tatagcgaac atattacaaa agaaataagt actataaaga
    12241 atagtataat tacagatgtt aatggtaatt tattggataa tatacagtta gatcatactt
    12301 ctcaagttaa tacattaaac gcagcattct ttattcaatc attaatagat tatagtagca
    12361 ataaagatgt actgaatgat ttaagtacct cagttaaggt tcaactttat gctcaactat
    12421 ttagtacagg tttaaatact atatatgact ctatccaatt agtaaattta atatcaaatg
    12481 cagtaaatga tactataaat gtactaccta caataacaga ggggatacct attgtatcta
    12541 ctatattaga cggaataaac ttaggtgcag caattaagga attactagac gaacatgacc
    12601 cattactaaa aaaagaatta gaagctaagg tgggtgtttt agcaataaat atgtcattat
    12661 ctatagctgc aactgtagct tcaattgttg gaataggtgc tgaagttact attttcttat
    12721 tacctatagc tggtatatct gcaggaatac cttcattagt taataatgaa ttaatattgc
    12781 atgataaggc aacttcagtg gtaaactatt ttaatcattt gtctgaatct aaaaaatatg
    12841 gccctcttaa aacagaagat gataaaattt tagttcctat tgatgattta gtaatatcag
    12901 aaatagattt taataataat tcgataaaac taggaacatg taatatatta gcaatggagg
    12961 ggggatcagg acacacagtg actggtaata tagatcactt tttctcatct ccatctataa
    13021 gttctcatat tccttcatta tcaatttatt ctgcaatagg tatagaaaca gaaaatctag
    13081 atttttcaaa aaaaataatg atgttaccta atgctccttc aagagtgttt tggtgggaaa
    13141 ctggagcagt tccaggttta agatcattgg aaaatgacgg aactagatta cttgattcaa
    13201 taagagattt atacccaggt aaattttact ggagattcta tgcttttttc gattatgcaa
    13261 taactacatt aaaaccagtt tatgaagaca ctaatattaa aattaaacta gataaagata
    13321 ctagaaactt cataatgcca actataacta ctaacgaaat tagaaacaaa ttatcttatt
    13381 catttgatgg agcaggagga acttactctt tattattatc ttcatatcca atatcaacga
    13441 atataaattt atctaaagat gatttatgga tatttaatat tgataatgaa gtaagagaaa
    13501 tatctataga aaatggtact attaaaaaag gaaagttaat aaaagatgtt ttaagtaaaa
    13561 ttgatataaa taaaaataaa cttattatag gcaatcaaac aatagatttt tcaggcgata
    13621 tagataataa agatagatat atattcttga cttgtgagtt agatgataaa attagtttaa
    13681 taatagaaat aaatcttgtt gcaaaatctt atagtttgtt attgtctggg gataaaaatt
    13741 atttgatatc caatttatct aatactattg agaaaatcaa tactttaggc ctagatagta
    13801 aaaatatagc gtacaattac actgatgaat ctaataataa atattttgga gctatatcta
    13861 aaacaagtca aaaaagcata atacattata aaaaagacag taaaaatata ttagaatttt
    13921 ataatgacag tacattagaa tttaacagta aagattttat tgctgaagat ataaatgtat
    13981 ttatgaaaga tgatattaat actataacag gaaaatacta tgttgataat aatactgata
    14041 aaagtataga tttctctatt tctttagtta gtaaaaatca agtaaaagta aatggattat
    14101 atttaaatga atccgtatac tcatcttacc ttgattttgt gaaaaattca gatggacacc
    14161 ataatacttc taattttatg aatttatttt tggacaatat aagtttctgg aaattgtttg
    14221 ggtttgaaaa tataaatttt gtaatcgata aatactttac ccttgttggt aaaactaatc
    14281 ttggatatgt agaatttatt tgtgacaata ataaaaatat agatatatat tttggtgaat
    14341 ggaaaacatc gtcatctaaa agcactatat ttagcggaaa tggtagaaat gttgtagtag
    14401 agcctatata taatcctgat acgggtgaag atatatctac ttcactagat ttttcctatg
    14461 aacctctcta tggaatagat agatatatca ataaagtatt gatagcacct gatttatata
    14521 caagtttaat aaatattaat accaattatt attcaaatga gtactaccct gagattatag
    14581 ttcttaaccc aaatacattc cacaaaaaag taaatataaa tttagatagt tcttcttttg
    14641 agtataaatg gtctacagaa ggaagtgact ttattttagt tagatactta gaagaaagta
    14701 ataaaaaaat attacaaaaa ataagaatca aaggtatctt atctaatact caatcattta
    14761 ataaaatgag tatagatttt aaagatatta aaaaactatc attaggatat ataatgagta
    14821 attttaaatc atttaattct gaaaatgaat tagatagaga tcatttagga tttaaaataa
    14881 tagataataa aacttattac tatgatgaag atagtaaatt agttaaagga ttaatcaata
    14941 taaataattc attattctat tttgatccta tagaatttaa cttagtaact ggatggcaaa
    15001 ctatcaatgg taaaaaatat tattttgata taaatactgg agcagcttta actagttata
    15061 aaattattaa tggtaaacac ttttatttta ataatgatgg tgtgatgcag ttgggagtat
    15121 ttaaaggacc tgatggattt gaatattttg cacctgccaa tactcaaaat aataacatag
    15181 aaggtcaggc tatagtttat caaagtaaat tcttaacttt gaatggcaaa aaatattatt
    15241 ttgataatga ctcaaaagca gtcactggat ggagaattat taacaatgag aaatattact
    15301 ttaatcctaa taatgctatt gctgcagtcg gattgcaagt aattgacaat aataagtatt
    15361 atttcaatcc tgacactgct atcatctcaa aaggttggca gactgttaat ggtagtagat
    15421 actactttga tactgatacc gctattgcct ttaatggtta taaaactatt gatggtaaac
    15481 acttttattt tgatagtgat tgtgtagtga aaataggtgt gtttagtacc tctaatggat
    15541 ttgaatattt tgcacctgct aatacttata ataataacat agaaggtcag gctatagttt
    15601 atcaaagtaa attcttaact ttgaatggta aaaaatatta ctttgataat aactcaaaag
    15661 cagttaccgg atggcaaact attgatagta aaaaatatta ctttaatact aacactgctg
    15721 aagcagctac tggatggcaa actattgatg gtaaaaaata ttactttaat actaacactg
    15781 ctgaagcagc tactggatgg caaactattg atggtaaaaa atattacttt aatactaaca
    15841 ctgctatagc ttcaactggt tatacaatta ttaatggtaa acatttttat tttaatactg
    15901 atggtattat gcagatagga gtgtttaaag gacctaatgg atttgaatat tttgcacctg
    15961 ctaatacgga tgctaacaac atagaaggtc aagctatact ttaccaaaat gaattcttaa
    16021 ctttgaatgg taaaaaatat tactttggta gtgactcaaa agcagttact ggatggagaa
    16081 ttattaacaa taagaaatat tactttaatc ctaataatgc tattgctgca attcatctat
    16141 gcactataaa taatgacaag tattacttta gttatgatgg aattcttcaa aatggatata
    16201 ttactattga aagaaataat ttctattttg atgctaataa tgaatctaaa atggtaacag
    16261 gagtatttaa aggacctaat ggatttgagt attttgcacc tgctaatact cacaataata
    16321 acatagaagg tcaggctata gtttaccaga acaaattctt aactttgaat ggcaaaaaat
    16381 attattttga taatgactca aaagcagtta ctggatggca aaccattgat ggtaaaaaat
    16441 attactttaa tcttaacact gctgaagcag ctactggatg gcaaactatt gatggtaaaa
    16501 aatattactt taatcttaac actgctgaag cagctactgg atggcaaact attgatggta
    16561 aaaaatatta ctttaatact aacactttca tagcctcaac tggttataca agtattaatg
    16621 gtaaacattt ttattttaat actgatggta ttatgcagat aggagtgttt aaaggaccta
    16681 atggatttga atactttgca cctgctaata ctcataataa taacatagaa ggtcaagcta
    16741 tactttacca aaataaattc ttaactttga atggtaaaaa atattacttt ggtagtgact
    16801 caaaagcagt taccggattg cgaactattg atggtaaaaa atattacttt aatactaaca
    16861 ctgctgttgc agttactgga tggcaaacta ttaatggtaa aaaatactac tttaatacta
    16921 acacttctat agcttcaact ggttatacaa ttattagtgg taaacatttt tattttaata
    16981 ctgatggtat tatgcagata ggagtgttta aaggacctga tggatttgaa tactttgcac
    17041 ctgctaatac agatgctaac aatatagaag gtcaagctat acgttatcaa aatagattcc
    17101 tatatttaca tgacaatata tattattttg gtaataattc aaaagcggct actggttggg
    17161 taactattga tggtaataga tattacttcg agcctaatac agctatgggt gcgaatggtt
    17221 ataaaactat tgataataaa aatttttact ttagaaatgg tttacctcag ataggggtgt
    17281 ttaaagggtc taatggattt gaatactttg cacctgctaa tacggatgct aacaatatag
    17341 aaggtcaagc tatacgttat caaaatagat tcctacattt acttggaaaa atatattact
    17401 ttggtaataa ttcaaaagca gttactggat ggcaaactat taatggtaaa gtatattact
    17461 ttatgcctga tactgctatg gctgcagctg gtggactttt tgagattgat ggtgttatat
    17521 atttctttgg tgttgatgga gtaaaagccc ctgggatata tggctaaaat atatgtttga
    17581 taaaaaatta ttcctgtgct actaagaaat tatttttata taataaatat tgagatttaa
    17641 ttaaaagtca tgtgttattg taatacatga cttttagtta aaatttttct atcatttaat
    17701 aatctattat tcttgactat tttataataa aattcatata tggaaatatt aatactaaat
    17761 aattaatagt tgataaaaaa tagataatat gctaaaagca aaaactaatt tagagccttg
    17821 taactgttta tttgcaatta taaaaacatc tttaaacata ttgactataa tataaaatat
    17881 taactataat acaaaacaat attaattaat tttctctaca gctatccctg gtatagttat
    17941 atttccaccc atagttgatt cataagttat agttccagca cttatacctc ttatagttat
    18001 gtaatcatcc tctaatactc ttgaaggagt tattgatttt ttataactac atagtacaac
    18061 cttatcataa tttccagaca cagctaatct tatttgcacc tcatcaccat cttcaataac
    18121 ttgaataacc ttaccttcaa attttacctt tttgtactta taatcatcag gtgttctagc
    18181 taattggtca taagtaatac cagtatcata tcctttcttc tcctcttctt cttttttgcg
    18241 ttgttcttca gcctttttag cttcttcagc ttttttagct tcttcagctt tacgttgatt
    18301 ttcagcttca atagctttct tttcgtcgtc tttcattttg aaccatggtt cagcatcaga
    18361 caatttgcta tttaaagttt ctatttctgt tttttgagaa tctataactg atgtttggtt
    18421 agaaatgacc tcctcatggt cttcagaaca agctggtgag gatatattgc caaaaaacac
    18481 gcctaaaata aatgccagta gagcaatacc ctttgtgctc ttaaagaatt taattatttt
    18541 cttagagctt ccttttcctt cattactaaa ttcgttacca tcattttttt tagaaaacat
    18601 aatacaatct cccttttatt agatatattt tattttaaaa gttaaaaact taaaataact
    18661 agttgactaa aaattatata ttcatatttt cctaaaata
//
LOCUS       DAMJWU010000069        18519 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMJWU010000069 DAMJWU010000000
VERSION     DAMJWU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.070"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            150..1310
                     /locus_tag="PC460_002532"
     CDS             150..1310
                     /locus_tag="PC460_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2601412.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1653..1814
                     /locus_tag="PC460_002533"
     CDS             1653..1814
                     /locus_tag="PC460_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601413.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2007..2636
                     /locus_tag="PC460_002534"
     CDS             2007..2636
                     /locus_tag="PC460_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF2601414.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMGGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2876..4135
                     /locus_tag="PC460_002535"
     CDS             2876..4135
                     /locus_tag="PC460_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2601415.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4394..5389
                     /locus_tag="PC460_002536"
     CDS             4394..5389
                     /locus_tag="PC460_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF2601416.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5635..6702
                     /locus_tag="PC460_002537"
     CDS             5635..6702
                     /locus_tag="PC460_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF2601417.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYNKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7236..9146
                     /locus_tag="PC460_002538"
     CDS             7236..9146
                     /locus_tag="PC460_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2601418.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9209..9832)
                     /gene="ytaF"
                     /locus_tag="PC460_002539"
     CDS             complement(9209..9832)
                     /gene="ytaF"
                     /locus_tag="PC460_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF2601419.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10020..10664
                     /locus_tag="PC460_002540"
     CDS             10020..10664
                     /locus_tag="PC460_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601420.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10878..11936
                     /locus_tag="PC460_002541"
     CDS             10878..11936
                     /locus_tag="PC460_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF2601421.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12040..13224
                     /locus_tag="PC460_002542"
     CDS             12040..13224
                     /locus_tag="PC460_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2601422.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13431..14144
                     /gene="rpsB"
                     /locus_tag="PC460_002543"
     CDS             13431..14144
                     /gene="rpsB"
                     /locus_tag="PC460_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF2601423.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14223..15134
                     /locus_tag="PC460_002544"
     CDS             14223..15134
                     /locus_tag="PC460_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF2601424.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15219..15923
                     /locus_tag="PC460_002545"
     CDS             15219..15923
                     /locus_tag="PC460_002545"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF2601425.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            15949..16506
                     /gene="frr"
                     /locus_tag="PC460_002546"
     CDS             15949..16506
                     /gene="frr"
                     /locus_tag="PC460_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF2601426.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16509..16712
                     /locus_tag="PC460_002547"
     CDS             16509..16712
                     /locus_tag="PC460_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601427.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16741..17475
                     /locus_tag="PC460_002548"
     CDS             16741..17475
                     /locus_tag="PC460_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF2601428.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17488..18267
                     /locus_tag="PC460_002549"
     CDS             17488..18267
                     /locus_tag="PC460_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF2601429.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7608 a         1852 c         3222 g         5837 t
ORIGIN      
        1 attaatatat gtcaatagtt aggtatatat gtatttaatt agcatgatat agataaaagt
       61 atttatttta attgtctatt atctgaaaaa gtctttactt agaaatcata aaaatcaaat
      121 taattaggaa aagaaagggt gtgtataatg tgattgagac tggatttgta tattgtagtt
      181 tcttggttgt attcgtaggt gtaatttttt ttatggttga taaattgaaa tggaaggtat
      241 ttgatgttat taatccaatg cttttaatat acttaggttc agctatactt ggatcatttg
      301 gtttatttgc acagaatgaa gatgttggaa cgtatcaaaa cataataaca gtgaacttcc
      361 ttccattaat gttagtgttt ttgttaattc aatgtgattt aagaaaaata ttaaagatgg
      421 ggcctaaaat gttattatca tttttctgtg ctacatttac atttatgctt ggatttgtta
      481 tagcattcat aatttttaaa aattcaatgg gtgcagattc atggaaaatt cttggggcag
      541 catctggttc atggataggt ggctcatcaa atatgattgc agcagctaat gctctaggag
      601 ttgcagaaga aggacttagt tatgcaatac ttatgggttc tataggatat acagtatgga
      661 tgtctgtttg tctaatgtct gtgaaatttg gacctaaatt taacaaatgg acaaagtgcg
      721 atacaagttt tatagatgag gttacatcaa aacttgaaaa agaaaataaa gataatacag
      781 cacctacgtt tgtagaactt atgattttac tttctgttgg gtttggagtt gcgtcttttg
      841 cacaaattgt atccaagata cttccaactt caggtatact taatgaaact atgtgggtag
      901 tacttgttgc atctacagta tcaatattat tgggtatgac aaaaatatca aagcttaaag
      961 ggagtacact ggtaggaaat gtgtttatgt atttactcct tgcagctaca ggtagtaaag
     1021 caaatttctt tggattgtca gaagcacctg tttacataat gtttggattg gtagtagtcc
     1081 ttgtacatgc tatttcattt atagggcttg ctaaactatt taaacttgat ttatttactt
     1141 gtgaagtagg aagtatagct aatataggag gagtaactac agctccaatg attgctgggg
     1201 ttcacaatcc tgtattaata ccagtaggtg tacttatggg gcttctagga aatattatag
     1261 ggacttattg tgctcttatg gtaactcaaa tattacatat gttagcttaa tgaaaatatg
     1321 gtataaaatt aataatttaa ataagaaaag tcatttcaat ttaagcagaa gcttttactg
     1381 aaatggcttt ttttgtttga caagataatc tatttcaaga agatgttttg acatatttag
     1441 tgtataatat tgtaaaattt actaaaaacg agatatattt aatttttata ttgaatcgaa
     1501 cgtctgtatg atgtataata aatatacaga acgaatgttt gcggaacggg tgttttttga
     1561 atttatttta ataatttatg aatatatatc agaatgcata aatatattta aatataaaga
     1621 ttaaaaagaa tttaaaggag tgttgataac ttatgataat tccatcaaga agaatacaag
     1681 atataacatt aacaaattta aaaaatggtg aagtaacttt aattgaattg gatgaaatat
     1741 acaagaagat gggattttta tttgttgtta gtgaaggcaa attgaaaaag ataaaaaagg
     1801 agaatcatca ttagaaatga atgaaataat aaatattata atagtttata aaacctaact
     1861 tatgtagaat ataaatactt aagttaggtt tttttgtgat aaagtttata agtactcaat
     1921 tttataagat aaataatggt tttaagtgaa aagtaaataa gtttaaattt tagtaagtat
     1981 tatattgcat aagaggtgat attattatga aaatagtaac ctacaatatt cataaaggta
     2041 tggattcaaa taatagatta actctaacta aaatggggtt atatttaaaa caattggatt
     2101 gtgatgtaat ttgtttgcaa gaagtattat atcctcaatt tttagcttta aaagctgttt
     2161 taaatatggg tggtgtcttt gctacaaatg taaaaaaagt aaatatgata tatggtattt
     2221 gtacatttac aacatctaaa atgttaaaca gtaatcattt tttcttaact agcaaaaaag
     2281 aacaacgggg agctctttgt ataactatag atgcatatgg aagaattata aatgtaataa
     2341 atactcactt gggacttgat aggcaggaga gagcaaaaca attagacgaa ataatagact
     2401 ataggaacag attagtgggg atagttattt tatgtggtga ttttaatgag aaaaatgtct
     2461 ttttaagtat gtttaatgat atggctattt cactaaacaa gtcttactta ccaacatttg
     2521 aaaaatcaaa ctctagaata gattacattt ttgttaataa aaatactgaa ctaaaaggat
     2581 acacagtaga aaaaatttac ctgtcagacc attatcctgt aataggttac atttaaattt
     2641 caatataaat gaaattagtt gttataaaat aaacaatttt tttgttaaga aatagtaaaa
     2701 gaaatataat aaaaaatatc aaaaataaaa ctaattataa gacataaatt tacaataaag
     2761 ttacatttga ttaaattcct tgtttaacaa aagctattca aatataatct aaggtagttt
     2821 tgaaatggga actctattat gcaaatttaa gctagtgtta aaatgaggtg aagttatggg
     2881 cagaagaaca acaagagttt caagattagg taaagaaaaa aaacgaagtg ttttaattgt
     2941 attggcagct ttagttctaa taatgggtgg atttacttat ttttttaaca gtaaatttct
     3001 gtacaatggg aaaatcgcta aaaatgtata tatagaagga gtaaatgtat ctgatatgac
     3061 taaagcagag gcattaaaag ctataacaga taagtatact ccagaagact taaatcttgc
     3121 ttatgatggg aaaaaatata caatatcacc aaaagatatt gatttaaaat atgatacaga
     3181 agatgttgta aaagatgctt atgaaagtac aaaaaaaggg tcctattttc aaaacttaaa
     3241 aaaatatatt gatataagag tgaataaggc aaatatgaag ataaaagctg aatatgatga
     3301 ggcaaaactt agttccaaag tgtcttctat agcagatagt ataaatgtta agatgaaaaa
     3361 tgcttctatt agtgttggaa gtggtggact aagttataca gactctgttg taggtagaga
     3421 atttgatttg gcagcaaata aagagtctat atataatatg ataaaaaata aagagcataa
     3481 aactttagaa cttaaagtaa atcttcaaaa accagatata acaactgaac aagtaaaaac
     3541 tgtaaattct gttataggtc aatattcaac tacttattca caagctgtag agggtaggtc
     3601 atataatgta ggattatcag caagaaaaac tagtgatgta ttattaatgc caggtgaaga
     3661 attttcatac aataaactta caggacctag taataaagca aatggttaca aagatgcacc
     3721 tgttatagtt tatggtaagc tagaacaatc agcaggagga ggtgtttgtc aaacatcttc
     3781 aacagtttat aatgctgctc ttctttcggg tatggagata actcaagtta caaatcattc
     3841 atctgcatct acttatgtgc ctaaagggag agatgctact gtgagcgatg gaggattaaa
     3901 cttaaagttt aaaaatccat ataagcatcc tgtatatatt aaaaattatg caggtggagg
     3961 aagtgtatca tcagtaatct atggtaattc tggtgacaaa ccaaatatat ctatagaggt
     4021 aaaacaaact ggtcagaata aatattctac atatcgaata tttaaagatt caagtggaaa
     4081 ggtcataaaa aaagaacata tatcaaatag ttcttataaa gaattaaaaa aatagataat
     4141 tttttagaga aatatttgta atataaaagg gagtgtctca aaatgaactt tttagtttat
     4201 gaggcactct ttttctgtga aatcaaatat attttcatca tttcaacaat acaaaagaag
     4261 cgtatctcta ttttgagaca gccttttttg taaaaattaa cactattagt tcttgtttta
     4321 atattacaat cattaatata tagaaaatat tcctatatat tggtataatt gtaaatagat
     4381 ttagttatat aaaatgtatg aggaggtgac atggttgaat tttataggca atagatatga
     4441 ggtagtaaat tctgatgacg ttttagaaat taacaaaatc tataaagcta gagatgtttt
     4501 ttacaggaaa aatgtgttaa taaaagttat aaagcacaat aattatatct gtgaagattt
     4561 tgtttcaaat ttaattgatg aaagtactgc tttagatgag ataaactctc cttatatact
     4621 aaaaataata gatgttggga ttcactgtac agaagaaaca acattatatt atgttgtaag
     4681 tgaagacttt aatggtatcg gactagatga attaattcta ggtaattatc ttcatttaga
     4741 agcaattgta aacataatga tacaagtttt aaaagcttta gaaatgatac ataggaatca
     4801 ttcatatcat ggtagtttaa aatcgagtag tataatagta gatacagaat acaatataaa
     4861 aatttgtgat tttggaatta caaaagcaaa taatggggtt aatacaagaa gctatggaaa
     4921 caggagatat ttatgcccac atcaactatg tataaattat actgataaag aaagtgattt
     4981 ttttgcaaca ggtcttattc tatttgaatc tatatttaaa aaattgcctt ttggagaatc
     5041 aaattcagaa gaaaaaatgc tacaatctat tgataaggga ttagattgga atagtataaa
     5101 agcaattaat ggaaatactg aacttataaa tgttataaaa aggcttcttg gaagaaataa
     5161 taagtatact agagccaatg atattattat tgatttaagt aaagtaatgt atgaaaaagc
     5221 atatatagaa gttgaagatg agaaagaaga agagcagaaa caagacaaag ttatacaagt
     5281 taaaaaagaa tataaaagta aaatgagaaa aaaattacat aaaaaacttg ttgttgccgg
     5341 tctagctata gtcatgattt caatgattat tattagttcc ataatataaa aattgtatat
     5401 taagtatttt aaaatcaatg tctataaagg atatacatag aaattttagt gtagtcttaa
     5461 attatgaaag gaagtggtta ttatgttaac aaaaaataat gaggtattaa acgtctagga
     5521 gtattttaac aaatagccac ctgcttttac agtttagaat aaaaaagctg tggctaaagg
     5581 tcacagcttt ttgctacttt ttaaaggcag gagaatatta aaaaggagca aattatgagc
     5641 aatataaaaa taatagcaag taataaatgt tggattgaag atatagcaag gaaacaactt
     5701 gaagatattt caaagttaaa tggaatttta agagtagtag gattacctga cttacatgca
     5761 ggtaaaatac ctgtaggttt agcagttgag actaaaaata ttatttatcc acatataatt
     5821 ggaaatgata taggatgcgg aatgacttta tttaaaactg gggttctaaa aaagaaattt
     5881 aaaaaagaca aatggattaa gtcacttagt aagattaaag atttgtccga tatagaaata
     5941 aaaaacacat ataaagaaga atgtccaatt ttaaatttag gaactatagg gagtggaaat
     6001 cattttgtag aaattcaatg tataagtgaa atttataata aagaacaatt tgaacaatta
     6061 aaattttcat ctgatgatat tatgatgtta gttcactgtg ggtcaagaaa ttatggagaa
     6121 gatattttaa aaaaatttta tgataaagat ggacttgaag ttgaaagtga gaaagctata
     6181 gattatatta aaaaacatga taatgcttta atgtgggcag agcgtaatcg tgaagctatc
     6241 agtaaaaaaa ttatgcagtc aataggtaca tctggagatg ttgaaacaat attctctata
     6301 aatcataatt ttatagagaa aagggaagat aaatttatac atagaaaagg tgctgtttca
     6361 agtgaaagag gagctgtgat tataccaggt tcaagaggca gtctatcata tatagttatg
     6421 cctactgaaa atacagagat atctttatat tctctatcac atggagcagg tagaaaatgg
     6481 tctcgttcag tttgtaaatc tcgccttaaa agtaagtata ataaagatac tataaaacaa
     6541 actaaattta aaagtcagat tatatgtaat gatgtaaatt tattatttca agaagctcca
     6601 gaggcatata agaatataga gcagattatt gaaagtttga tagaatatga acttattcaa
     6661 gtagtggcaa ctatgaagcc attgattact tataaaggat aatttttaaa gttaaaaaag
     6721 attaaaatta tattataaat agctaatttt atggaacatt taaaagtgtt ttattattag
     6781 ctattttttt attttgtaga gacgataatt tatagagcta aattctttga tatataatga
     6841 ttacaaagtt agactgtatt ggaaaatgca catacatgaa ttgatgacaa tataagtgat
     6901 tcccaaatat aaatgaaagt ataagcttat tttcactata aaaattttca ctgtacaaaa
     6961 acattaatga cattattttt attgacataa atatataatt tatggaataa tattagaagc
     7021 actgatataa gtgtttaaat aagaatagaa aaggagaagt gttttatgaa aaaaaatact
     7081 aacattaatg agtccggagt taaacttctt aataaataag gagtttgata tataataact
     7141 ccttatttat taagttgttc gcaaaataac tattatcaaa gtaaatgata gggtgattta
     7201 aaagacgata acttataaat aaaggagaaa aaataatgaa tagtaataca tttgaaagat
     7261 tacaactaaa tgaagttaaa gaattaataa aaatacattg tgtaagttca ctaggaaaaa
     7321 atttaataga taaattaact cctagtggga atattggagt agtaagaagg aaattagcag
     7381 aaaataaaga agctagaaag ataattgaaa atagtaatca cataccactt gaaggattgt
     7441 ttaatgcagg ttctacaata gataaaattg aaaaaggaat gataatagaa cctgttgaac
     7501 ttgtaaatat tgaagatttt ttaagaggtt gtaggaagat gaaagctttt atgttagaaa
     7561 aagaatttta ttcacctaca ttaagttctt atgccttaaa tataacagaa tgtaaaagta
     7621 tagaagatga gataaattac tgtataaaat ctaataaagt tgattctaat gctagtaaag
     7681 aattaaaaaa gataagacga aatatagaaa ttacagaagg aaagataaaa gatagattaa
     7741 ataaatttat aacatcaact gtaaataaaa agtatataca agagtttata ataagtaaaa
     7801 gaaatgatag atatgtaata ccaattaaat cttcttataa aaatgaagtt aatggtacta
     7861 tattagatac atcatcaaaa ggaaatacgg tatttattga acctattagt gtttcaaatt
     7921 taagtacaga gttaactatg ttaaaagctg atgaaactat tgaagaatac aaaatattat
     7981 cttatttaac agaacttata tttgagaaga taagtcagat aaaattaaat atagagatac
     8041 tttcagaata cgatatggta tttgcaaaag ctaagtatag tcaaaaaata aaaggaatta
     8101 ctccaaaaat aaataataat ggctatataa aaataattaa aggtaaacat ccacttctaa
     8161 caggagatgc agtaccttta gattttgaaa taggcaaaaa ttatagaagt cttataataa
     8221 caggtcctaa tgcaggagga aaaactgtaa cccttaagac tgttggttta ctaactttaa
     8281 tggttcaatg tggatttgat atttctgcaa aagaaggtag tgaatttagt gtatttgaaa
     8341 aagtttttgt agatataggg gataatcaaa gtattgaaaa tgccttaagt actttttcat
     8401 cacatataaa aaatattgca gagattatga gtttatcaaa taattctact cttgttctgt
     8461 ttgatgaaat aggaagtgga actgaaccta atgaaggagc tggtttagca atatcattat
     8521 tagaagagtt ttacaaaatg ggatgtataa ctatagcatc aactcattat ggagagataa
     8581 aaaagtttgc tacattgcat ccagaatttg aaaatgctgg tatgatgttt gataaagaaa
     8641 cgttagaacc attgtataag ttaactattg gaaaatctga agatagtaat gctcttttta
     8701 tttctaaaaa aatgggaata aaaaatagag ttttagatag agctaaagaa tatgtaattg
     8761 atagaaacta caatttagat ttagttaaaa gaagtaagct acaatctaat attgaagata
     8821 aaaatagttc tgaagtcatt ttaaactata cagattataa tgtaggggat aaggtaaaat
     8881 tactagatga agatgatttt ggaattgtat ataagcctat ggacaagttt aataatgtag
     8941 aagtattgtt taatgataat tttatttcag taaatataaa aagattacaa cttagcataa
     9001 aagcatcaga attgtatcca gaagggtatg atttagatgt attatttact agttttaaag
     9061 aaagaaaatt agaaaaagat attcaaagag gttcaaaaaa agctcttaaa aagattcaga
     9121 aagaaattag agagaataga aaataaatat aaaaagcaca aaagaaagca atctaaagag
     9181 aaaagtttat ctttagattg ctttcttatt aacatatctt cataaatcca atgactaaca
     9241 agattaaacc acttaaccaa gataagttta aatttacgtt tttaacaaac atatttccta
     9301 gtaaacaacc aagtaatact gcaattatat ttgatattaa agaaaataga ataacttgta
     9361 tataattcac acctacaagg ccactaccaa aaccgatagc taatccatct aaacttaaag
     9421 caagtgcaag tgtaaatgct tcagcagatt taagcacttt agatttactt gtatctaaga
     9481 catcaggttc aatacttaca ttaaaattta atttaaggtc taacatatta aaattataat
     9541 tctttgagtg tatagatttt ttcttaaaat aagatttaaa aaaactttca attagtctag
     9601 cacttcctaa taaaaataaa ataaagaaac atattaacat tgttatattt tgaggtaaaa
     9661 agtctctaac tattgtacct gcaaaaagcg atattgctaa aatagctgaa caaactacgc
     9721 tgataattat atttgaatat aagggaactt taactttatt tacgccatat ccaaagcttg
     9781 tcacaaaggc atctatacaa agagaagtaa ctaataaaat tgattcaatc acagtagatt
     9841 aacatccttt ctcttaaatt ttgtacttat attagttata gtattgtaaa aatggataaa
     9901 ttgttacaga taatccaata aattgttgat aaaattccaa catcatacaa cacctaaata
     9961 acaaaagaat catatcatat aataatataa aaccaatcat aataaagcag gtgaaaataa
    10021 tggaacaaca tagtaaggtt gggaatttcg gtgatttgtt gaaagtttta ttagatgaaa
    10081 aagaattatc aatgggagaa ctaagtaaaa aaagtggaat tgataaatct actatatctc
    10141 gtatagctaa taataaacaa aagccaaata taaatcattt agaaaagatg gcaatacatt
    10201 taaatattaa cctagaagaa ctgttgaaag cttcaggata tgaatttaga aacagcaata
    10261 atcaaatatt taatgcagat agtgatttta gtaactttga tgatattctt ggatttgcaa
    10321 atttaatcaa tgataagaac tttaatggaa atattgaaaa agagttaagt aagtgtaagt
    10381 tatatgtcca aacagatgaa ggtaaaaaat tactttttga taattttaat aaaaaaatag
    10441 attctattga aaaacaaggt caatttacag atagattaag acaaatgtat gctgattttt
    10501 gtacagatgg acttacaata aagaaatatt tattgattgg aagtatgcta ctttactttg
    10561 ttatatcaac agatgtgatt ccagacttcg tgttcccaat aggatttatg gatgatttag
    10621 tagcattaaa tatagttaca aaattactta aaaatgataa gtaagtttta gaatcatatt
    10681 taatatataa ataaatcaaa gtatataaaa tttattattt tttatatgaa acaaattaaa
    10741 agcacaatga agtagattaa aaatttaaaa ctaagaatct aatatatatt ttgtaactat
    10801 ttaagtatat aaaataaaat aatatccaaa ctaatacaaa atagtaataa ttgttttgta
    10861 ctggaggata tatatttatg aattgtaaat ggatatctaa aatagatgag agaaaaaaat
    10921 gtcatagaga agcagattct tctggatatt gtatctttca taaagaaaat aagtctgatg
    10981 aagagataca attgatgatg gatactcttc ataaagaaga gataagtgaa tttaatggat
    11041 ttgtttttga aaatgaattt aatgcggaag aaatattaac ttacaattat aaaatactgg
    11101 atttttctga atccatattt aaacaaaaag caaattttaa aaaatatatt tttaaaaaga
    11161 atattatttt taactataca gaatttagag ataaagtttt atttaatggt tgtgtattct
    11221 tagaaaactg tgattttaat agaaccatat ttagtaaaca ctatataaat gaccgtatat
    11281 ttgaaaaagt gaaatttaaa ggacctgatt tggtagttaa taaagtagaa aattttccac
    11341 gaatggatgg aataattttt agcatgtgta caaaatttgt gttaaagaat gttgaatatg
    11401 gaaaaagtga gtatgagcat ggcaagataa attatagaat agctagaaat caagcaacaa
    11461 aaattggaga atatgagatg ataggatttt actattataa ggaaagaatt tatagtagta
    11521 aaataatgaa acgttctaat tatcctacct tcagtgatta tttagttgaa aaattttttg
    11581 accaaatagc acgttacaca acaggttatg gagaaaagcc atggaatata ttattagtta
    11641 ttatagcaat aataagtgtt tttgctttat tatacttatt tgtaggcata gaaagttcaa
    11701 atagcacact tgtagcttta gatataaata atataggtga ttactcacta agtgagattt
    11761 tcaaaatgta tatggattta tggtatttta gcatggctac atttagtaca gtaggatatg
    11821 gagatatggt tgctacaagt ttgattggga aagcattggc aggtatagaa gtattttttg
    11881 gagttacaat aggagcaatt tgggcatctg tgattattaa aagaatgata aggtaatatt
    11941 tgtgtaaata tggataggta ttaattacta gatataaatt ggactaaata gggaaaattt
    12001 aaatataaat atacttaata atccaagaag gtgaaaaatt tgaaaaatgc actaaaaaaa
    12061 attatatcat tagccatgat tctagttttt ataataccaa attattcata tgcagatgat
    12121 aaatctgtag acaagtattc aaaaacatct atacttatag accaagaaac aggtagagtt
    12181 ttatatagta aagaacctga tatgaaggta ccattagcta gtttaagtaa gatgatgact
    12241 tttttacttg caatagaagc tattgaaaat aaagaagtaa aagaaactga taaggtaaaa
    12301 atagacaaat ctattgcatc agtaaaaggg tcaagttata aacttaaaga tggtgaagaa
    12361 gtgccactta tagagcttat gagaggtctt atgattgtat caggaaatga tgcggcaatt
    12421 gctatagcaa aacatgtttg taaaacgaaa gaagcatttg tagatagaat gaataaaaaa
    12481 gcaaaagaaa taggaatgag taatacacat tttctaaatc caaatggtct tccaatatac
    12541 gatttaagca accccaaaaa accagcaaaa gaaaatatct ctactgcaaa agatatagct
    12601 attcttggaa aatacatgtt tgaccattat gaaaaacaag taacagctat aactgatatg
    12661 gagacatata caaattcaga tagaagtttt gaaaaaagca atacaaatgc attattaaga
    12721 attataccag aagtagatgg tataaaaact ggatatactg gcaatgctgg atattgctta
    12781 tcattttcta tggttgtaaa taaaaatgat aaaaatgaga aaaaacgtag agtaataggg
    12841 gttgtattag gtactaacca taaaaataaa agaacctctg cttcattagc tctactgaaa
    12901 tatggaaaag aaaatttcaa tatgaaaaaa gtaattgata aagatagctt tattggtaag
    12961 aaatatataa aaggaacgaa agagttagag gttacattaa aagcaaaaga tgaactttat
    13021 acaatattaa aagatgatga aagtataaag tcagaagtta agattaaaaa tatagagtac
    13081 cctgttaaaa aaggtgatac tatgggtatt ataaaatatt atactaattc aggtgactta
    13141 cttggaagtg tagatatagt aagtaataat agtatagata acgtatcatt aaaaacaaaa
    13201 ttaaaaatga aatttgcaaa ttaaattcaa aaaatggtat aaaatacaca aaaaaatata
    13261 aaaatagaaa atatggaaaa aatattgtta tttcttcacc tctgtgatat actactagag
    13321 gtgaaaaaat aaaataaatc acacacagca ttgggatcct taaaaggtgc ctattacatg
    13381 gttttttagg gagaagatca agctggaggt ataaaaccag gaggtaagat atgtcagtaa
    13441 tatcaatgaa acaattatta gaagcaggtg ttcactttgg tcaccaaaca agaagatgga
    13501 accctaaaat ggctaagtat atattcacag agagaaatgg aatatatata atcgacttac
    13561 aaaaaacagt taaaaaagtt gaagaagctt acaagttcac taaagaagta gctgaaacag
    13621 gaaagccaat cttattcgta ggaactaaaa aacaagctca agatgcgata aaagatgaag
    13681 ctgaaagatg tggaatgtac tttgttaatg aaagatggtt aggtggaatg ttaacaaacc
    13741 acaaaactat aaaaacaaga ataaataaat taagagaatt agaaaaaatg gaagaagaag
    13801 gcgtatttaa tgttcttcct aaaaaagaag ttataaaatt aagagctgaa aaagaaaaac
    13861 tagaaaaata cttaggtgga ataaaagaca tgcctgaatt accaggtgca atgttcgtag
    13921 ttgacccaag aaaagaaaac atagctattc aagaagctca tagattaggt ataccagtag
    13981 ttggtatagt tgatactaac tgtgacccag aacaattaga ctttgctata ccaggaaatg
    14041 atgacgctat aagagccgta aaattaataa ctggtgctat ggcaacagct gtaattgaag
    14101 gtagacaagg tgctgaggaa gaagtagctg aagaccaaga ataataattt gacaattaaa
    14161 tggtaagggt cgcccttacc atttataata tggaatagaa taagtaggag gaaacttaaa
    14221 aaatggctaa tataactgct caaatggtaa aagagttaag agaaagtaca ggcgcaggaa
    14281 tgatggactg caagaaagct ttacaagaag ctgaaggaaa catggaaaaa gcagtagatt
    14341 tattaagaga aaaaggatta tcaaaagctg ctaaaaaagc tggtagagtt gctgctgaag
    14401 gtctagttgc aatagaaatg aatgatgata atacagttgc ttctatggta gaagttaact
    14461 cagagacaga tttcgttgct aaaaacgaag acttcaaagt gttcgttaaa gatgctgctt
    14521 gtatggcatt agctactgat aaagaagata tagcttcttt attaggtgaa actcataaag
    14581 aaggaataac tttacaagaa gttttaaata atagagttgc aaaaataggt gagaaattag
    14641 actttagaag atttgctaaa gttgtaacta atggacaagt tgctggatac attcacggtg
    14701 gtggaaaaat aggtgttctt gttgaaatgg aaacagaagc tagagatgct aaagttttag
    14761 aattaggtaa agatgtagct atgcaagttg ctgctatgaa tcctaagtat gtttcaagag
    14821 atgaagtaga tgctgaatac atagcacatg aaacagaagt attaactcaa caagctttaa
    14881 atgaaggaaa accagctaat atagttgaaa aaatggttaa aggaagatta gaaaaagaat
    14941 taaaagaggt ttgtttatta gagcaaactt tcgttaaaaa tccagatata actgttaaac
    15001 aattagttgc tgatgttgct aaggcagtag gttctgatat aaaagttgtt aaagttgtga
    15061 gattcgaagt tggtgaaggt atacaaaaga gagaagaaaa ctttgcagaa gaagttgcta
    15121 agcaacttaa gtaataacca aagagagaac acgcaggtgt tctctttttt aaaaaataga
    15181 aaaaattgga tatgaaggat tgaaatagga ggtttctgat ggataaacca atgtacaaaa
    15241 gggtattgtt aaaattaagt ggagaagcat tagcaggaga aagaggtttt ggtataaata
    15301 atgatgtagt taatgatatt gctattgcta taaaaaaaat acaagaaata ggtgttgaag
    15361 tagctgtagt agtaggtgga ggaaacttct ggagaggcag aaccagtgaa ggaatggaca
    15421 gaactactgc tgactatata ggaatgttag ctacagttat gaatgcaatg gcgctacagg
    15481 atgcactaga aaatatagat gttgctacaa gagttcaaac tgctatagac atgagacaaa
    15541 ttgcagaacc atatataaga agaagagcag ttagacactt agaaaaggaa agagttgtta
    15601 tatttggtgc aggaacagga aacccttact ttacaacaga tacaactgct gctttacgtg
    15661 ctgctgagat ggaagcagaa gttatattgt tagctaaaaa tgttgatgca gtatatgaca
    15721 aggatccaaa agtacatgca gatgctaaga aatttacaga attaagttac atggaagtta
    15781 tacagaaaga attaaaagta atggattcta cagctacatc tttatgtatg gataataaaa
    15841 ttccaataaa ggtatttgag cttacaacag agaatataat aagagcagta aaaggcgaaa
    15901 atataggaac tactgtaaaa taatgaagaa tgaaggagga gtaaaattat gaaactggaa
    15961 atacataagc aattagaaga gaaaatgaat gggacaattg atgctttaaa gtttgaattt
    16021 ggaactataa gagcaggaag agctaatgca caaatgctag acaaaataag agttgattat
    16081 tatggaactc caactccaat aaatcaaata ggtgctatat ctgtaccaga accaagaata
    16141 ttaatgatat ctccttggga taaatctgct atgcatgaaa tagaaaaagc gatagctaac
    16201 tcagacttag gattaaatcc atcaaatgat ggtgaggtta taagactttc tgtacctgct
    16261 ttaacagaag aaagaagaaa agaattagct aaaaaagcta gtaaagctgc agaagaattt
    16321 aaagttagaa taagaaatga aagaagagac gccaatgaga aaattaaaaa gatggaaaaa
    16381 ggcggagaat taactgaaga tgaattaaaa aaagcacaag atgaagttca aaaaatgact
    16441 gataaattta taaaagaaat agatacatta ttatctaaaa aagaaaagga cataatggag
    16501 gtataatatt gaaaaaatat tataaccttt taggtcttca tgtagatgag gtaaaacaat
    16561 attttgatga gcaaaatata aattataccg taaaatctat tgaaggtaaa aaggataaag
    16621 aaaaacttgt agttcctaga gttataaaaa tatctgagat aggtgatagt gtagaactga
    16681 taatcacata cttttctgac tccttagttt aaggagtcag aacaatattg gaggcaaaat
    16741 atgaataaca acattatata tgatatagac ttaaataaca tacctactca tattgctatt
    16801 ataatggatg gaaatggaag atgggcaaaa gctagatttc ttccaagaac tgcaggacat
    16861 aaggctggag tagaaactat aagagatata gtaaaagaat gctcaaaatt aggtgttaag
    16921 cacttaacat tatatgcatt ttcaacagaa aactggaaaa gaccgaaact tgaagttgac
    16981 acacttatga acttattatc tacatactta agaaatgaaa tagctgaact tcaccaaaac
    17041 aatgtaaaag taactgctat aggagatata agtgctttac caaaaacttg tataagagaa
    17101 ttaaactcag ctaaggaact gacaaaaaac aatacaggag ttaacttaaa tttagcatta
    17161 aactacggaa gtagagcaga tataaaaaat gctcttatag atatagtaaa aaattgtgaa
    17221 agtggtaaaa tagatataaa caatattgat gaagatatta taaaaaacta tttaagcaca
    17281 aagtctatac cagatccaga cttggtaata agaacaagtg gtgaacaaag attgagtaac
    17341 tttttacttt gggaagttgc ttattctgag ttttacttta ctgatataca ttggcctgat
    17401 tttaaaaaag aagaactaca gaaggctata tacgtatatc aaagaaggga tagaagattt
    17461 ggaggactta agtaaggaga ttattttatg cttacaagaa ttattgcttc actagctctg
    17521 gttccattat ttctatttgt tgtatatgga ggtattcctc tatatatagc tgagacagca
    17581 atagtttata ttgcattgca tgagttttat aaggcattta aaataaaaga tgtacatcca
    17641 atattcatta taggatattt attttctatt tatttagctg taaaaaatat ttttaattta
    17701 ccattagaat atacttatgc agtaattttt attttatttt tagcaagtat aatttatatg
    17761 ttgatgggca aaaacaatgt tatagatgtt tcgattacgt ttttaggagt tttttatata
    17821 ggagtatttt tagattttat aattataact ataaatggat ttgaaaaagg tagtatatat
    17881 gtatggctta tatttgtgat atcttttatg acagatatat ttgcatattt tagtggttat
    17941 ttacttggaa aacataagct aattcctaaa gttagtccta aaaagactat agaaggagct
    18001 attggaggaa taataggaag tacactttgt tgtattttat ttggatattt atttggcatt
    18061 gatttgcttc aattagcgat aataggaagt ataggaagtg taatagctca attaggagat
    18121 ttatttgcat cttctataaa gagatatgta gggataaagg attatggaaa gataatacct
    18181 ggtcatgggg gtatattaga tagatttgat agtgttatat tagttgcacc ttttgtatat
    18241 agtgcgatta aattttttat tagataaaga ttggttggat ttaaaagagc aatctataat
    18301 tgctagtaat gaaaatagct atgtaacttt taaatgcaga gatattctgc agttattagt
    18361 tacatggcta ttttcattgt tcaaaaattt agaagacctt ggggaattgt ataagaatag
    18421 gtattacttt ataatagtat actatatttg tagtgagtat attttttata ctataactat
    18481 cattaaaaaa tatttttttg tttaataatt tcgatattt
//
LOCUS       DAMJWU010000070        18513 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMJWU010000070 DAMJWU010000000
VERSION     DAMJWU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.071"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(<1..529)
                     /gene="yqeC"
                     /locus_tag="PC460_002550"
     CDS             complement(<1..529)
                     /gene="yqeC"
                     /locus_tag="PC460_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF2601430.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIEN"
     gene            complement(712..1989)
                     /locus_tag="PC460_002551"
     CDS             complement(712..1989)
                     /locus_tag="PC460_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2601431.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            complement(2128..4434)
                     /locus_tag="PC460_002552"
     CDS             complement(2128..4434)
                     /locus_tag="PC460_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2601432.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            complement(4427..5221)
                     /locus_tag="PC460_002553"
     CDS             complement(4427..5221)
                     /locus_tag="PC460_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2601433.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            complement(5328..6686)
                     /locus_tag="PC460_002554"
     CDS             complement(5328..6686)
                     /locus_tag="PC460_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2601434.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            complement(8034..9041)
                     /locus_tag="PC460_002555"
     CDS             complement(8034..9041)
                     /locus_tag="PC460_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601435.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEITVMKEDMSEVKQ
                     EINVLKVDMSEVKQEMTVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEM
                     TVMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNF
                     MQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLL
                     HNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSV
                     K"
     gene            complement(9271..10356)
                     /locus_tag="PC460_002556"
     CDS             complement(9271..10356)
                     /locus_tag="PC460_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2601436.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(10392..11705)
                     /locus_tag="PC460_002557"
     CDS             complement(10392..11705)
                     /locus_tag="PC460_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2601437.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLNENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDTKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(11706..13997)
                     /locus_tag="PC460_002558"
     CDS             complement(11706..13997)
                     /locus_tag="PC460_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2601438.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGSDKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(13987..14781)
                     /locus_tag="PC460_002559"
     CDS             complement(13987..14781)
                     /locus_tag="PC460_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2601439.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(14820..15266)
                     /locus_tag="PC460_002560"
     CDS             complement(14820..15266)
                     /locus_tag="PC460_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2601440.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(15338..16744)
                     /locus_tag="PC460_002561"
     CDS             complement(15338..16744)
                     /locus_tag="PC460_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2601441.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(16762..18132)
                     /gene="hydA"
                     /locus_tag="PC460_002562"
     CDS             complement(16762..18132)
                     /gene="hydA"
                     /locus_tag="PC460_002562"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2601442.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEEIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(18265..>18513)
                     /locus_tag="PC460_002563"
     CDS             complement(18265..>18513)
                     /locus_tag="PC460_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2601443.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIKTIG
                     FGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSVYGK"
BASE COUNT         5559 a         3612 c         1967 g         7375 t
ORIGIN      
        1 agttttcaat acttactgta gaactactat taatactact tgtatctaaa tttgctattt
       61 ttttaaatat ttctactcta tgtatattct cttcattaat attcattcca aatgatgtta
      121 tatctaatat ccctatggtt tttgtagttt tatgatatac tactggttct ttagcattcc
      181 aacctttaag tttttttctc tttgagccat ctccctctat taaaactaaa tcaaattggt
      241 ctacaatttc atccaaaata gaggaatcaa tacttactaa tttattttca ttatttatat
      301 aactgccaca gactgttatt ccatgacaaa tagatggaat aacagttcca tctattgtaa
      361 taattatatt gtcataatcg tttggtacat atattttagt tgttgttgta aggagaactt
      421 tcaatttatc tctataaaaa tttgcaaaat agtttataaa cgaagttttt cctcctgctc
      481 caactacagt tattatttca ttcttttcta ttatatttga tagttccaca ctttcacctc
      541 ttataaccag ttttctaatc tcttaattat tatagttaga cctttttagt aaaagtgatt
      601 tttgaaattc cataaactcg aaagtaaata gattctgttg taaacaacaa ggcggatcca
      661 attcgccgct aagctgtaag cttacgctat cagtttatct tttaaatata attaagatat
      721 tcttactctc tgtggttgct ttaatatttg aggattattc tgtaaattag ataaattaac
      781 tacttgtgat ttttttagta tattaagact cgcatttaca tctgcattta taatgattcc
      841 aacactggat ttgtactgtc cacgcgttat tctttcccct ttaaagtcat attttttaac
      901 attgtccaca ttgatatcag gtagaaaatc atcagcaaaa aaatctgatt tactagtata
      961 gctttcttct tgcctaataa aattgatatt ttttatttta cacagatatt ctaattttcc
     1021 ttttatctct ccaaatggga ttcttataaa atttctatat atttttctct ccatattatc
     1081 acttttttct aacttagcat tatatcctac aattaaattt ccaatattat tttttacaca
     1141 ataatcaatt atataaaaac aggttttatt tacatagtct ttaacttgat tatttctctt
     1201 attccaaact ttaaattgtt ttcttgttat tgcagttata ttctttttta attttaaggc
     1261 ttctatttta tcattctgtt tgtttgccca ctgattcaca gattttagcc tttttccatc
     1321 tatgataaaa ctatcaccta aattggtaac acatgtacat aaattttcta aacccaagtc
     1381 tatagctagt gcattttttt gatttgaaac ttcctctgat tttggtattt cataacaata
     1441 ctgaatttca aagaacctag ctttatgttt aggtattatt cttatttctt taattttttt
     1501 ctctagtata ttttgtggaa cttttatact tactttccca tatagttttt taaatgctgg
     1561 agacataggt acatttaaaa tcccatcttc tttgattctt atttgaccta ttattatgct
     1621 aaaaaatcct tctttatcta aatatttagg tgattttatc cctctaaagt catattttcc
     1681 ttctttggct aatttaatta atccaaaaaa tgacctaaac gaactgtcta cttcttttaa
     1741 tatttgttgc gctatattac tattgagaag cttatagttc tcattttctt tgcatacatg
     1801 ataattagct tcatagttaa gatgttctcc ttgattgaaa taatgctgtc ttacattata
     1861 tgtaccaaca ttatataagt tcttagctaa gaagcacatc tctcgtagag cttgatattc
     1921 ttttttagtt aagtttttta attgttgtct ctgagttcca tagtgtatat tttttattct
     1981 gtttgacatg aactcacctc ctttagaata tactctacta gacattatat acggtatttt
     2041 tacaaaacac catatataat gtccatttaa ttactttatt ttacaaaata atttaaattt
     2101 acttgctttt atacacatta ttatttatta taattgttta ttattatttt tcatacccat
     2161 ataaactttt tcaaaagtca taggaagctc tcttaaacga actcctgtag cattaaatat
     2221 tgcatttgct atagctggtg ctggtgtatg tataactatt tctcctaatg atttagcacc
     2281 ataaggacct cctgggtcga aattctcagc aaaatctacc aatatatttc caacttcttt
     2341 ttttgtagga actttatact gtaggaaact atctgttaat aatctaccat ctttatcaaa
     2401 tatagggtct tcatacattg caaaccctat acccatcatc acaccacctt ctacttgtat
     2461 tctagctaaa gttggattta taactgttcc acaatcaggt acacatgcaa aatttatgac
     2521 tttatattcg cctgtatttt tatctaattc tatctctgca aatcctgcta taaatggttt
     2581 tatttttttc aacatgccaa aagattctgt tgcttccaaa actactggta catcatcact
     2641 agcaaatcct attcctccaa cagattctct acccaattcc tctaatagaa caaacttgct
     2701 attatcgttt ttaacaaaaa ccctgtcttt tcccaactct aaatcttctt ttttaaatcc
     2761 tagctttgac tctgcaactg aaagtaatat atcttttaat ttatttgctg tctttattgt
     2821 tgcatttcct gtaaccgttg tcgtacaaga agcaaatgct cctgtatcat agggacataa
     2881 gtctgaatca cctgtataaa tagaaattct atctacagta gtatttaaag cttccgcagc
     2941 aatttgagct tgaattgtat cagccccttg acctaagtca gcaagaccag ataatagttt
     3001 atatgtgcca tcttcatcca ttcttatagt gactgttgcc atatctacac gcggtatacc
     3061 agaaccatga tttgctactg ccatacctac tgctcttact ttgttgtttc ctatatcttt
     3121 acatggatat ttatcatccc aattaattaa tttcttgccc ttttctatac attgtctcag
     3181 ctcacaactt aatatttcgt cttcagctgc ttttccattg gtatacctat ctattatatt
     3241 tttaattcta aattcaagtg gatccattcc aatcttatgt gccaattcat caatagcact
     3301 atctattgca aatattccct gtgtagcacc ataccctcta agtgctcctg ctggtaacct
     3361 attagtatat actgttctag cattaaatct aacagcatca atatcattat atattggtaa
     3421 ggtattgtgt ccagattcgg aagaaacaga gacaccattc ccaccataag cacctgtatt
     3481 gtttaatgac tttagctcta ttgcttttaa atttccttct ttatctgcac caactttaac
     3541 atcaaaaaac atttcatgtc tactattaga acctacaaaa gtttcacttc ttgtaaatac
     3601 aaattttgct ggtctaccag ttttcatagt aacaaatgca gtaaatatat ctgttgctgc
     3661 tatattcttt cctccaaatc caccacctat tctaggttta attactctta tttttgaaga
     3721 tggtataccc aatacttttc taagtattct ctttacattg tgtggtgatt gtaatgatga
     3781 aactacaaca attctatcat ttatatcttt atatgaatat gacctatgag tttccatcat
     3841 gcaatgcgat tgactttggg ttttgtatga agattcaacc acaaaatcac tttctttaag
     3901 cttagcttca acatctccaa attcaaaaaa ttcttttgat actatattat tttttgcatc
     3961 caatccaaaa ggatatggct ctactatgtc tttttctgga tgtataacaa cttcatttcc
     4021 ttctgcttct cttgcatcaa acacaggctt taaaacctca taatctacct ttatcatttt
     4081 taaggcttta agtgccgttt tctcatcttc agcagccact aaagctactt catctccaat
     4141 aaatctaact gtatcttcaa gtaaaactct atcataaggt gatgcttctg gttctgtttg
     4201 acctggcaca caatatctga cctgtggtac atctttatac gtataaatac ccacaacccc
     4261 tggaattttt tcggcttttg atgtatctat attctttatc ttagcatgag catgtggact
     4321 tctaagtagt tttaatgtca aaacatcttt atgcataatg aggtcttcag tatatagagg
     4381 ttttcctgtg acaattgctg tactatctat ctttcttatc cccttattaa tcattataca
     4441 ttccctcctc aatagcatta tacatccttt taactaatgc acttgccata tcctttctgt
     4501 actctttact agctctcata ttacttccat aagtaagttc cttactagca acaatagatg
     4561 ccttatctat atctttttct ttatttaata tattactcgc ttcaagtgct aatctagctt
     4621 tttgaggtct tgccccaatt gctattttgt aagtatcatt ttcttttagc attgctgaat
     4681 ttaatacaga aaaatctcct gtgcattttc ttattgagtc aaaaacagct attgcatctt
     4741 tttttggtag tattacttcg accaatatat ccctacttaa ttcactatcc aaaaactcac
     4801 ttaactctag tattccttta ttgtataatc tcaccttcgc tccaacaacc aaaagcgctg
     4861 gtattaagtc agaaaagcca tactttgaaa atacacttgc accaattcta gccccttgtc
     4921 taaattgaac accaactata ttagaaacag cgcttgaaag tattccacta cagtaatttt
     4981 ttatagtttt atcaatttct aaacttctaa gtgaagtatc tgcacctatc aaaatatttt
     5041 ttttatcctc ttttatataa tttagattta tatcttttaa atctatcgct gttcctatat
     5101 ttttattact taattttata aatccacatc cacctaaaat aacattattt tttttagaaa
     5161 ttaaaatttt atatgcttct tctaaactct gtggaactga atattccttt atagtaacca
     5221 tcttatcccc ccatcattaa cttattaaaa cacaaaagtt tttaataaat cttttattta
     5281 tatttataat tgtagtatat ttaattcaca tatattaaac ctttacatta taaactttta
     5341 tttttatcct caaccttatc acttttattt ccaggaaatt ttaccatata taaagcttga
     5401 attattatgg ctaccattgc tcctgaaact acacttgaat ctgcaagtaa tgtctctata
     5461 aattttggaa atttttcaag aacttgagga gctaccatga atccaatacc agaagccata
     5521 gaaagaccta atatcatgct attttcttga ttaaatccat ccatactaac aagtcttaaa
     5581 ccagatgttg taagagttga aaatataacg agtgttgctc ctccaaccac acagggagga
     5641 attgttctta ttaaattagc taataaaggc gatattcctg ccaaaaataa tccaattcct
     5701 ccaatagcta gtacaaattt acttacaacc tttgtattgg aaacgattgc agaattttgt
     5761 ccaaacatcc ccattggagt tgcagaaaat aatgcaccta ttgtagagag tactccattt
     5821 cctaaaacag cagaagaaag ttcttcatct gttacttgtc tattaagccc tcctaccgca
     5881 gaaagtgtac aagcacccat tatatcagct atctcaacta aatatattat tgaaaacatt
     5941 acaacaactt caagtcttat atctagccca aaagcaagtg gttttggaag tgcaaccaaa
     6001 gtaaactcct gaattgaaga aaaatttact atacccaaaa ttaatgataa aatgtatcca
     6061 aaaattatgc taactaatat tgatgcatct ttaaataatc cttttccata cttatttaat
     6121 cctaatatca taagagatac agatattcca actataaaat taattggatt cccaaaagtc
     6181 tcattgccca tacccccagc taagtttttt atagctattg gaaaaagtcc aagacccata
     6241 catgcaacta ttgttcctga tacagctgga gtaaatataa atcgtatttt tttaagacca
     6301 aaaccaatta taattattac tataccagct attatttgag agccaaataa tactggtagt
     6361 ccatacttac ttgcaacaga taccccagct cctataaaca tagctgtagc tcccattata
     6421 actggtattc ctcctcctaa tttaaaacct ttaaacaaag gtattggata taattgtaat
     6481 aaagttgata atcctgcaaa aactaatgcg gcttgaatca ataatgttga catcttatga
     6541 ttaagtccag ctgcatttgc aatcaatatc gctggtaaac aactacctat taccattgcc
     6601 attaagtgtt gtaaccctaa aggtattgct tcctttagtg gtggtattcc gtctaattca
     6661 aattttgatg tactttttgc tttcataata aaaactcccg ttacataaaa cttattgaat
     6721 tatataaata cttaaaaatc taatatatta aattaatgat gttcataaat atcaactata
     6781 ctccaacata tatttaagtt tttagtctta aaaaacagat gcaataaatt taattattaa
     6841 caaataaacg tattctttaa ttttgtatac attatatacc atgctttaca aatataagag
     6901 tattatagct atgaattttt aattttttat agtaaattta attcaattta aatctttcaa
     6961 tcttaatcat tataagttat aagttttcat aagtatttac atgtttcaag ttttaaacat
     7021 ttacctataa acgctacttt atagatgtca cttcaaatcc agtgacctca tactttcata
     7081 tgagcgtaga ctatttcttc atcttcagca ttacctgcta agagcaaccc gtttccacta
     7141 tcaatcgctt atagtgtact ctctctcgag atagtcgttt gaccttccta tttctaggct
     7201 tggcgaccaa atgtccattt ttaagtactt aggctctcac catataccat cctcactatt
     7261 gtttctggtt ttcaccacat tcataaaagt ttctttctat cttttattgt agttgcaagg
     7321 ctttaggatt tactggtttt aaagttgtgt cctacataga tttctctatg tacggggcca
     7381 aaagtacaat ttatttaaca tactttgctt taccccctgt aaataatatt atttactctt
     7441 tcactgtttt tgttattaaa ttaattaaat atcaattaat ctaagcataa aataattatt
     7501 taatttaaac tcaaaatatt tatgaatatt ttataatata attctcataa gacagaaaaa
     7561 cttaataagc tatgtatctc agttgtacca atgtactctc ctttactata tttgtatctg
     7621 caacataaaa tatacaagtt tctatctatt tatagatata tatatctata agcaaaatag
     7681 taatattatt tttaatagta aaaattttat tcatattact atttataatt tttatagagc
     7741 aattttagtg ccaatatttg catacatttg tatagtataa aatttttatg ttctcattga
     7801 taaaatttta cttatttcaa gcgttctatt agattttatc atctaaattt taggttttat
     7861 ttttataaat taattgtatt tacataaaaa aaaagaccta aaaacaaatt tttaacggtc
     7921 ttttctcaat tttttatttt catttctaaa atttataata aatagtaaga acactataaa
     7981 ttcttttata taaaattctt agtttgtgga tgataaatta gtgaattaaa atactacttt
     8041 actgatttta gtttgacaat atcactccaa tttttactag tggctaactc tactaaattt
     8101 aagtctcttc ttatttcagc tatatcctct tttatagaat tagtgtcacc ttttatatat
     8161 gctatgttat gttctatatt atcatgagta gctttattta tttcagaatt atgtaataaa
     8221 cttcctaaaa tattattctg ttctatctgt atctctttca ttgatttatc ttcacattct
     8281 agactatcaa tcttaatttc aattccatat aatttttcat ttgctatgga tacctcgtcc
     8341 ttaattccat tcatatcttc attaatgtta tttattttat tttgcataaa gtttacttct
     8401 gatttaactt cggaaacatc ttctttcatt acagttattt cttgtctcac ttctgacatg
     8461 tcttttttca ttacatttat ctcttgtttt acctctgatg tatcttcttt cattacagtc
     8521 atttcttgtc ttacttcgga catatcttct ttcattacag tcatttcttg tcttacttcc
     8581 gacatatcct ctttcattgt atttatctct tgtcttacct cagacatatc ctctttcatt
     8641 atatttatct cttgtcttac ctcggacata tcttctttca ttacagtcat ttcctgtttt
     8701 acttcggaca tatctacttt taatacattt atttcctgtt ttacttcgga catatcttct
     8761 ttcattacag ttatttcttg ttttacttcc gacatatctt ctttcattac agtcatttct
     8821 tgttttactt ccgacatatc tccttttaat acatttattt cctgttttac ttccgacata
     8881 tcttctttca ttacagtcat ttcttgtttt acttccgaca tatcttcttt taacacattt
     8941 atttcttgtt ttacttccga catatcattt tttatgctaa ctacatcttc atttatagta
     9001 tataataact ctaaaatctt ctcttgcact tgcatcacca tccctattaa ttattataac
     9061 attttacaca aatgctatac aatgttaaca tatatatcag aattattaaa attttaatta
     9121 aacaaaattg attcacttta ttacttcaaa actaattaat atctaaaata actacaaata
     9181 aacaaaaata aggtcaacaa caaaaaaggc ttatctcttt gagatagcct ttcttatttt
     9241 tatttaaaac tttgtttttt ataaaataat ctatacattc agctctgaac atgaaatttt
     9301 tcttccaagt gcttttggtt tacaaactga tatacaaagt ccacaatatc tacactcttc
     9361 ttcattcaat tccatcttat cattcaattt tctcgcttga tatgcacaaa ctctctcgca
     9421 catcttacat ttagtacatt tttcttcatc ataagtaaac tcaaagctaa gtctttcatt
     9481 aacatctccc atatgattat gtactatacc acaagcattt tctatagtat tatatcctaa
     9541 tctattaaga ttagatttta aatcatcatg tattttagat attacttgtg cgccttttaa
     9601 aattactact gaacatatac caacaaagtc agcacctgcc ataatcattt ctaaggcatc
     9661 atctccactt acacatccac ctaagccaat tattggtata ttaacttgtt tttttatttc
     9721 atatattctc tgtaaagtta ttggcttgat agtttctcca cttaaatatc ctattccatt
     9781 tccacctaat aaaggttgcc ctgtttctat gtctattcta aatgctggtc ctacagaatc
     9841 acaagcagtt attgcatctg ctcctgcttc ttctaatttt ttagcaaaat cgcctatctc
     9901 atctatcata ggaggaagtt taactattac aggtatattt actctttcct ttgcatcttt
     9961 aagcattggt atcaaatctc tatagtcata agatactaat tcaataaagt cagctcctgc
    10021 atttgcaact ttctctacta attcgctact ttcttcaatt gtatgaccta ctgaagctat
    10081 acagacagtc ccatctcttt tcatttgtgg tatttcaaaa tctatccatt gctctggctc
    10141 aaaatctgtc catttttcat tatttaaaag agcattagct gtatttctaa ccatatgtgg
    10201 tgctggattt atagctcttt cttttcttat agtcttagtc acaactgccc ctgctcctgc
    10261 atcacttaat atcttgcatc cagctgcact atatgttaat ggtcctgaac ctattataag
    10321 tggtgatttt aattctttac ctaaaaaatt atacattatc tgcttcccct tttcatatat
    10381 atttacattc gttaaaattt ccaaaattca caagcaactt ctttcatttc tttaacaata
    10441 gcttttttat ctaaatcaac taactgtttt tcttttaaaa tatgtctacc atctatgtat
    10501 acatttgata taaattcttt tttaccctga actactatct ggtcaaatat attatcaaga
    10561 gtcacaggag ttttaaattc atcttccata actattatat cagccttatt accaactgct
    10621 attttaccag aattttgaag tcctaataca taagcaccat gctctgttgc cattctaaat
    10681 actaaatttg ctggcattac tgatgcatct tcttcaactg atttatgcat taaaaatgca
    10741 cctctcataa cttcaaaaaa atcatttata tatccgtcag tacccagtgc cacttttata
    10801 ccttttttaa tcatttttgg tactggagaa aaacctccac ctacttcaca gttgctaaga
    10861 ggcatatgaa ccacttttac acctttttct tttaatatat ctatttcttc atcatttact
    10921 tttacacact gagatgctaa tacagtttca tccaatatat ctaaatcatt atagtagtca
    10981 actgcttttt tgccaaaatg tttttcagca tagtttggct catatatact ctcacataaa
    11041 tgaaattgca ttggtgcatt taattttttt gtatcttctt tagctttctt aataaatcta
    11101 tcagaacatg tgaagcttgt atgtgtacac attattccat ttattaattt actattttct
    11161 ctactccact ttattaaatt agaattttca tttaaacatc taagtccatt ttcaaagctt
    11221 attctttcac aactttctaa ggataaaatc gctttcatgc ctatttcttc tagtattttt
    11281 ccttgctcaa ttaaagttcc ttcttctgta tttggagctt ctagggtatc acaaaaagct
    11341 accacaccag attctataag ttctattgct gatgctttag ttgttgcctt cacttctttc
    11401 aatccaattc tattttctat aaatggccac caatagtcat ttaagaaacc ttcaaagtcg
    11461 gtaaaatgta catttgccgg tattccccta gaaagaactc catattgatg catatgacca
    11521 ttgacgaatc ctggcattat tatagcatct gatttatcta ttatttcttc aatattttta
    11581 tattttattt caacttcttc atttggcaat atatctttta taatgtcatt ttctactaca
    11641 acagcagaat ttgtatatat ttcatcagca gaagatatta agtatttact tttaatagca
    11701 atcatttatt tacctccctg atttaatttt ttcatggcca tataaacttt ctcagaagtt
    11761 ataggaagtt ctcttatacg aactcctgtg gcattgtata ttgcatttgc tattgctggt
    11821 gcaggtgtat gaataactat ctctcctaaa gatttagctc catatggacc tgttggctca
    11881 taattatcta caaaatcaac aattatgttt cctatatctt tttttgttgg aactttgtat
    11941 tgtaatagat tagaagtttg aagctttcca tcagaaccat gtctagggtc ttcatacatt
    12001 gcaagcccta taccttgagt tacaccacct tcaacttgta ttctagcaag tgcaggattc
    12061 ataactgtac cacaatctgt agaacaagca taatttatta ctttaaactc ccctgttgtt
    12121 ttatctaact ctatttcagc aaatcctgct aagaatggtt tagcactctc ttgtatacca
    12181 taagattcag atgaatataa aacttcttga tttccaccac caacagattc ttttcctaat
    12241 tcacttaaca aaacaaactt ctctacatct tcactataac aaactctgtc ttcatatagt
    12301 attaaatcat ctattggtaa atcgagtttc ttctgtgcta cttttaatat cttttgtttt
    12361 aaactttcag cagattttat tgtagcattt ccagttacat aagtagtaca agaagcgtat
    12421 gcaccagtat catatggaca catatctgta tctcctgtat aaacggaaat tctatcaata
    12481 gatgtattca atgcttttgc agctatttgt gcaagtattg tgtcagaacc agttcctaag
    12541 tctgaagacc cagaaaacaa cttataagtt ccatcttcat ccatcctcat atttactatt
    12601 gccatgtcta tgttggctat accagaacca tgagttccca cagacattcc aacagccctc
    12661 accttattgt ttccaatatc cctaacaggg tacttttcat cccatccaat aagtttttta
    12721 cctctaatta tacattcatc aagtgcacaa cttcttattg gaaaattcat tattccgcct
    12781 tcagtttgtt tttttataat attctttaac tttatttctg ttgggtccat attaagttta
    12841 tgcgctaatt catcaattgc acaatcaagt gcaaatgtac cttgtgttgc tccatatcct
    12901 cttaatgctc ctcctggaac taaattagta tatacagttc tcccatcaaa ttttattgca
    12961 ggtacattat tatatgttgg aagtacatta tgacctgatt cagagcaaac tgaaggtcca
    13021 ttatcgccat aagcgcctgt attgttcaat gctttcatat ctatagcttt tatgtttcct
    13081 tctttatctg acccaatttt tatatcaaaa aacatctgat gtctagtatt agtcattgca
    13141 aaagtttctt ctctagtata aattagtttt gctggtcttt ttgtcatcca agttacaaat
    13201 gatgcatata tctctgttac agctatgttt tttccaccaa aacctccacc tattcttggt
    13261 tttattactc tcacttttga aagaggtatt cccagtgctt tagaaacctg tcttctcata
    13321 tgataagctg attggtttgc agaagttact actaatctgc cattcgtatc tatatacgta
    13381 taagctctat gagtttccat catacaatgg gcttgtgatt gagtcatata agaatgttct
    13441 actattacat cactttcttt aaactctttt tctacatccc ctttacccat tttttctctc
    13501 gatacaatat tttttgtagc atccaatcca aaatcaaatg gacaaaacaa atcatcttct
    13561 tcatggatta aaattttatt tccttccgat tctcttgcat ccaatatagg agtcattact
    13621 tcatattcaa cttttataag tttcatagct ttaatagcag ccctttcatc ttcagcggct
    13681 attatagcaa cttcatctcc tacatatcta actacgtctt caagtattaa ttggtcatga
    13741 ggtgatgctt ctggatatga ctctccaacc attgaatatc tataatttgg aacatctttg
    13801 taagtgaata tgtttactac accttttacc tttaatgcat tttttatatc tatgtttttt
    13861 atcttcgcaa aagcatgtgg acttctaaga agcttaattg ttaaaacatc tttatgaaaa
    13921 attaaatctt ctgtatatat tggttttcca gtaacaatgg cttcactatc tatttttctt
    13981 acctctttat ttaccatcac actcacctcc aatactatta tacattctca ctactaaggc
    14041 ttttgccata tcttttctgt attctttact tcctcttata ttacttccaa atgaaagttc
    14101 ttcacttgat acttcaccag ctttttctat atcattttct aaacttaata tttcacttgc
    14161 tttataggca atttttgctc ttcttggtct agcgcctatc gcaattttat aaatacttcc
    14221 ttcttttaac attgctccat ttaatactgc aaaatcacct gtacattttc ttatagaatc
    14281 aaatacacct atagcatctt ttttaggtaa tataacttct attaatatat ctttttcaag
    14341 ctcattttct aaaaaatcag ctaagttcat aacccctttt ttatataact taacctttgc
    14401 atctaccact agtaatgaag gtattaaatc tgaaaatcca tacttagcaa atacgctagc
    14461 tcctactcta gctccacttc taaattgaac tcctactata ttagaaacag cgtttgaaat
    14521 taccccatta cagtaatttt taataacctt atttaattct aaagttctta aacttgtatc
    14581 tgctccaatt aatatgctat catcagtttc atttatataa tctaaagcta agtcttttaa
    14641 atctattgct gaaccaatat ttttactacc tagttttaaa aatccacatc ctccaagtat
    14701 tatgttgttt tttcttgata ttagcagttc ataagcttca tctaaacttt ttggaactac
    14761 atattccttt atagttatca tactctctcc ccatatcaac ttaattttca cttaaaagtc
    14821 taagtgttta aatatttatg tattgctctt aattgacctt tatatcctgt acatctacaa
    14881 aggttaccat ttacataatc tataacttct tcttctgttg gattttgaag ctctcttttc
    14941 attgccaaga ccatcattat aaatccagga gcacaaaatc cacactggtc agccccttcg
    15001 tctgcaagaa aatctccaaa cttctttgct tcatccaaga caccctcaag tgttgtaata
    15061 tgctgacctt ctactctagc agtaagataa ttacatgaaa gcacggcctt gtcatttacc
    15121 atcactgtac ataacccaca tgagcctgta tcacaccctt ttttaacact tacatatcca
    15181 ttttctctta atgtatctgc taaaaaatca tctggtctta tacttaattc tttttcttta
    15241 ttatttatgg tacatttgat aatcaaataa atcacctcaa aactcatatt tttaagctaa
    15301 agttttacta tactttttta tgaataaatt acatttatta ataaaggcta ttccatttat
    15361 gaccttccat tctatttttt atagttcctc ttacacaaag tctctcagct gtatcttgag
    15421 catcttttaa tgctttctct tcattagctg tcaatatttt tgaatcttcc attattatat
    15481 ttccatctac tataacactc tctatatttt tcatactaga agaatatact aatgtagata
    15541 ccggattatg cataggtatc gcttttggtg aaagcattgg attaaatatt aataagtctg
    15601 cttttttacc tatctccaat gaacctatct catcttccat tccaatagct cttgctccat
    15661 ctatagttgc caactcaaga actttttctg ctgacattgc taatgggtca cacttattaa
    15721 ctttatgttg aagtgctgtt aatttcataa gttcaagcat atcttgagag ttattactag
    15781 cggctccatc tactcctaaa ctaactgtta ttcctttttt aagcatttct ggcactggtg
    15841 caacacctga agataagtac atattacttg ctgtattatg agacactttc atatcgtatt
    15901 ttttagtaag ttccatatct ttttcagtta gatacacaca atgtaccata agcacatttg
    15961 gtccaagtat tcctaatttt tctaaaacat ctatgtcata ttgcccatgc aattcttttg
    16021 ctgcttctct atcaaaagga gtctctgata tatgtactgt aaataaagca tcatcatatt
    16081 cttttactac tctccatagc atttcaagca tttcttgaga atttgaccat attgctgcag
    16141 gagctactcc tattttgatt cttccatttt ctgtattatg gtgtttttca aacaatcttc
    16201 ttacatcttt ttcaaccgtt tctacatctt gcattattcc aggatgaact ccaaattgag
    16261 ctcctgtatt catacatcct cttccaagta ttcctcttat tcctaattct ttataagcat
    16321 ctattattcc atcacaaaga cctggcttac tatgaggata catgtagtca accattgttg
    16381 ttataccact tctaagtcct tcaatacatc caagcattgc agcatcataa gtatcttttg
    16441 gctctaaata gttagctgct ggaaatgtca tagtttctaa ccagtcttta agtaccatat
    16501 catctcccaa tccttttaat aaagtttgga ataaatgatt atgtgtattt ataaaacctg
    16561 ggaataaaac tttaccttta gcatctataa tttttttaac atcagtatat ttactttcta
    16621 tctctttaga atttccaata tcagcaatct tgttatcttt tactactaat gccccatcaa
    16681 aaataacttc tctctcttta ttcacagtta ctattatggc attcttaata agtatatcca
    16741 tcattcttat ttccccccgc attatttagt aatttttctt ttaatatatt gacctgttgg
    16801 atgttcttta acattacaac tttgtactac atgatttcct ctcaaaaata catcttcaac
    16861 tttacattta accttaaatc cttcatatgg agtataatct acattttgtt tttgagtttt
    16921 ataactaatt gtttcttctt tattgatatt taaaatgact atatctgcat cacttcctac
    16981 agctatctca ccttttttag gatacattcc aaatattttt gcagcattcg tagaagtaac
    17041 ttccacaaac ttatttgcac ttattctatt ttctaagact ccatatgtat ataaaagtgc
    17101 taacctatgt tcaacaccag gtgctccatt tggtatctta ctaaaatcat caattcccaa
    17161 gtctttttgc cccttaaaat taaatgaaca gtgatctgtg ccaactgttt gaatatctcc
    17221 actagacaat ccttcccaaa ggtaatttat gtcttctttt tttcttaaag gaggagacat
    17281 tacatactta gcaccctcaa atttatcctt attatataaa ttgtcttcta ataatagata
    17341 ctgtgggcat gtttctacta tcatatctat atttttttct ctagccattt ttacagtttc
    17401 aagtgactcc ctacaactta agtggactac ataggatttt gaattagata atttagttat
    17461 atcagcaagt cttgaaacag actctttctc aactatgtta ggtcttgtaa gtggatgata
    17521 ttttggctcc aaattaccat tttgtatatt ttcttctatc aatacatcta gtaaatcccc
    17581 attttcacaa tgaaatccaa ctatacaacc taaatccctt gctttcttta aaaccctgta
    17641 tattgttcca tcatctactt tcatgccatc atatgccata tacatcttaa aagatactat
    17701 cccatccgaa ataagttttt ccatatgatt aaatgtatct ttatttaaac atgtaattgt
    17761 catatgaaaa ccataatcac aataacaatt tccacttgct tttttatgat atgcttctac
    17821 accttgcagt aggttttccc cctcattggc ttctgcaaaa tctagtattg tagtagttcc
    17881 tccagctata gctgctcttg ttcctgtttc aaaattatct gcagttgtaa tagaacctgc
    17941 attcatatca aaatgagtat gtgtgtctat tccaccagga attacaattt tacctgtagc
    18001 atcaataacc ttatcaccat ttatttctaa gttatttcct atttcaacta tctcttcatc
    18061 ttctatcctt aaatcactta tataagtctt atcagaggta attatagttc cacctcttag
    18121 tattgttccc atatatacct cctaactttt aagctttaaa gtattttcta agtattgtaa
    18181 aactttattt aaaattgtat tttctaaagg tgctatattc agcacctcta gactatattt
    18241 aaataattta ttgtgaacta aatattattt tccgtataca gattttaata ttccatagta
    18301 accttctgtt cctataaata actgttcttg ctctatatac tcatctattg tgtgagcaag
    18361 attttcctta gaaggaccaa atcctatagt ttttattcca gcttcacctg catagtgact
    18421 tccattcgta cagaatgagt attgagttat ttctgaatct attccagctt cttttaatcc
    18481 cttatatgct gcttgaacaa attcatcttc ttc
//
LOCUS       DAMJWU010000071        18422 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMJWU010000071 DAMJWU010000000
VERSION     DAMJWU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.072"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            198..1049
                     /locus_tag="PC460_002564"
     CDS             198..1049
                     /locus_tag="PC460_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2601444.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1053..1523
                     /locus_tag="PC460_002565"
     CDS             1053..1523
                     /locus_tag="PC460_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601445.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            1536..2846
                     /locus_tag="PC460_002566"
     CDS             1536..2846
                     /locus_tag="PC460_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2601446.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            3058..3804
                     /locus_tag="PC460_002567"
     CDS             3058..3804
                     /locus_tag="PC460_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2601447.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4166..5707
                     /locus_tag="PC460_002568"
     CDS             4166..5707
                     /locus_tag="PC460_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2601448.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            5742..6848
                     /locus_tag="PC460_002569"
     CDS             5742..6848
                     /locus_tag="PC460_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2601449.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(7179..8465)
                     /locus_tag="PC460_002570"
     CDS             complement(7179..8465)
                     /locus_tag="PC460_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2601450.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(8588..11686)
                     /locus_tag="PC460_002571"
     CDS             complement(8588..11686)
                     /locus_tag="PC460_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2601451.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSSDFEKLLY
                     QK"
     gene            12411..12563
                     /locus_tag="PC460_002572"
     CDS             12411..12563
                     /locus_tag="PC460_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601452.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(12856..13560)
                     /locus_tag="PC460_002573"
     CDS             complement(12856..13560)
                     /locus_tag="PC460_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2601453.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(13551..15158)
                     /locus_tag="PC460_002574"
     CDS             complement(13551..15158)
                     /locus_tag="PC460_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF2601454.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(15412..16590)
                     /locus_tag="PC460_002575"
     CDS             complement(15412..16590)
                     /locus_tag="PC460_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601455.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(16816..17310)
                     /locus_tag="PC460_002576"
     CDS             complement(16816..17310)
                     /locus_tag="PC460_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2601456.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(17311..18072)
                     /locus_tag="PC460_002577"
     CDS             complement(17311..18072)
                     /locus_tag="PC460_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2601457.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         6279 a         2993 c         2361 g         6789 t
ORIGIN      
        1 aatatgtaat attttagata tttttcgttg acaatgttaa atttttgttt tatccttaat
       61 taaggacatg ttattgacat ttctgaaact tatttgaaat agttttttca ttttatttag
      121 gaaaacgtat tatacctaag aaaacgtatt ttctcatttc agataagtaa aatatttatt
      181 cttttgggga ggaaatcatg gaaattaata aaaaacattt tattcaagta tttttaactt
      241 gcttaatctt ggtagtaata tgtgaattta tagggcaaag aactatagct attggaaaag
      301 cctctatagt actgttccct atgctttatg cagttataat tggacttata ataacaccag
      361 atttattagg taaaaaaatt aaagcactta aaaaagtggt tggagaaaaa gaaataaata
      421 ttgctggtga agttgttgga attgcactag taatgcttgg tataaaatat ggtactacag
      481 ttggaccaaa cattgacaaa ataattcaag ctggaccagc atttatcgct caagaatttg
      541 gtcatatact tgcaccaatc gttgcattac ctcttgcatt aatgtttgga atgaaaagag
      601 aagctgttgg agctactgca agtataagtc gtgaaccttc tttaggagtt atatcagaaa
      661 aatatgggat aaactcacca gaaggtagtg gtgtacttgg tacatattta atgggaacag
      721 ttcttggtac tatatacttt agtgtactag gttcaatatc tatttatagt ggtcttcacc
      781 catatgcttt gggaatggcc tgtggtgttg gtagtggaag tatgatgaca gcagctgctg
      841 gttcattatc taccatggta ccacctgaaa tggcagatac aatacttgcc tatgctgcaa
      901 caagcaatat gatgtctagt ataacaggaa ttatattctt agtttttgta tccttaccat
      961 ttactaattt tatgtataag attttagaac ctaaattttc gagaaataaa aaaactaaag
     1021 aaattaaatc tacagaagga gagtgctaat aaatgtttgc taatttaaat ttaaagaaaa
     1081 gttcatatca attattggtt atgtctgtta ttggagttat aattcttata ggacaaagaa
     1141 ttagcgttgg tacatctata gttacagctc ttcctggtat ggtgatgcta atattagctg
     1201 ctatggcagc tatgattata aaagatttat ttcctaaatc aatctttcca gcttttggat
     1261 ttgcaactat aatcggcctt ttacttagta tgccatatag tccaacatct gaagtattct
     1321 taactaacac taataatatt aactttatgg ctataactac tccactacta gcttttgcag
     1381 gaatttctgt tggaaacaaa atagaagctt taaaagaaat gtcatggaaa atagttataa
     1441 tttcacttat tgtatttaca actatattct ttgcatgtgc gagtatagct catatagttt
     1501 taagtattca aggtaaaata taggaggaat ttattatgaa taaagaacaa ttaaaatctc
     1561 tagtaataaa aactatagat gaaaatagag aaaaaatatt aaaaataggt caaggaatat
     1621 acaaaaatcc agaatatgga tataaagaat ttaaaactac agaagctgtt tcaaactttt
     1681 ttgagcaaga attaaattta tctgtagaaa aaaatatagc agttacagga tgcaaagcag
     1741 atgcaaattc tagcaagaaa ggtcctcata tatctatact tggagaattg gatggtatat
     1801 catgtaaaga acataaagat gctaatgaaa taggtgcttc tcatacttgt ggacataata
     1861 ttcaaatagc tggaatgtta ggtgctgctg ttggtttagt aaaaagtggc gttttagaac
     1921 atttagatgg taaagtatct tttatggcta ctcctgctga agagtttatt gagttggaat
     1981 acagagagca attaaaaaaa gaaggaaaaa taacttattt tggtggaaaa caagaattaa
     2041 taaaaagagg ttattttgat gatatagaca tatctatgat gtttcatgct tctgatttag
     2101 gagaagataa ggctctagtt ggtcctgaga gtaatggatt tgttggaaaa aaggttaaat
     2161 ttataggtaa agaatctcat gctggttctg ctccacatga tggaataaat gctttaaatg
     2221 cagcaatgct tgctataaac aacgtgaatg cacttagaga aacttttaaa gagtctgaaa
     2281 gagttagatt ccaccctatc atcacaaaag gtggagatat agtaaatgtt gttcctgctg
     2341 atgttcatat ggaatcatat gttagagcta gaactataaa tggtatgatt gatgctaatg
     2401 aaagaataaa taaggctttg atggctggtg gaatggcagt tggagctgat gttgagataa
     2461 ctgaaattcc tggatatctt ccaatattac gatacaaaag tcttgataat ttatttaaaa
     2521 acaatcttga agatttaggt ctaaaaggaa aagttattga tggtggagat tttacaggct
     2581 cttttgattt tggagatgtg tctcatataa tgcctactct tcaccctatg attggtggtg
     2641 taaaaggagc tcttcataca agagattttg aaatagttga tgaagattta gcttacataa
     2701 ttcctgctaa agcaatggca cttacagttg tagaccttct ttttgataat gctaaggatg
     2761 ctaaagatat attagatacc tttactccag ttatgactaa agaagaatat ctaacattct
     2821 tagaaaaaca cgataaaact ttataagtct atttttataa tagggtggta tctactgtaa
     2881 aagttatacc actctttgtt tttgtaacag tatacttctt ttcttttagt ttttagatta
     2941 aactaagata tattatcctc cttaaaactt ttagagctac gttgaaatat cagtagaact
     3001 atgctatatt ctttctaatt aatataaatt tctattctaa ttcaaggtgg tgaaaaaatg
     3061 tttaatgcag gaaatcgaat tataaataca tggttgtacc ctattcaaga tggctttgtc
     3121 ttgattgata ctggatatga aaatggtttt aaacatttca aaaaacgaat ttctaaattc
     3181 aacataaaaa tcaaagatat tcgatacata tttctaactc atgctcatga tgaccatgtt
     3241 ggttttctaa atcagattct agacgaatct cctaatacaa agattgttat gcatgataaa
     3301 gctttagaaa ttctatataa aggtcaaaac tcttttattg gtggttgtac cagccgtact
     3361 gcctttctct tttgccaaat tatgaaacta tttggaaaag gtgagcatag tttttctcca
     3421 cttaaacaag aatttgaaaa aaggtgtatt cttgtgtctg acaagaatcg taaagaaatt
     3481 gaaaaagaac taaatggaaa aattatagat acaccaggac atactgctga ttcaatttca
     3541 ctactaattg atgaaggtgt tttattttgt ggagattctg caatgaatgg atttccaagt
     3601 actcacaaaa ttaccatatt tgcagaaagc aaactagaat ttattcaatc ttggaaaaca
     3661 atcatcaata caaaacctaa aatgatttat cctggacatg gaaaaccatt tgaatacagt
     3721 aaattagaac aaaatctaat ctatgtaaat aaaataaatt tatatcctct acataattca
     3781 aaagatattc acaggttttt gtagaaaata caaagtaaat ttatttgatt aattaaaaaa
     3841 tctaacactt tactacgcta cttattttaa taacttaatc ttccactagc ttttcataaa
     3901 attttgtttt aaaattaatt ttaaagcgca tatgttattt aaatacatta gataaaattc
     3961 gtcattaatc tacttaaagc atatttaact aacaccactc aaccagaaaa ttcagaaaac
     4021 tgcattgaca caggaaaaag tttattttat acttttttta ggacatattg tggacacgtc
     4081 ttaaatcgac aaacagaatt taaattgtaa gattaaccac aatacttatt ttttaattat
     4141 atttttaaac attagggagg atattatgaa tgataacgta gttaagaaga aaaacttttt
     4201 tgacaggaca ttaaacagaa tagaaactgt tggtaataaa ctaccagacc cagttacaat
     4261 atttttaggg ttatgtgtac ttttacttat actatctagt ctagttggtt ctatgggtat
     4321 atcagtagtt catcctggta ctggagaaac tataaccgct gtaaatttat tgactgtaga
     4381 acagcttcaa attcttttag gaaacatagt cagcaatttc caagggtttg ctcctttggg
     4441 tctagttcta gtaactatga taggtgctgg tgtatgtgac aaaacaggac ttatgacagc
     4501 aacaatcaaa gcatcagtaa gcaagattcc tgaaacaaga gtaactcttg ttgtaatgac
     4561 tataggtatg cttgccaata tagcttcaga tgcaggtaca atattatttc caccattagc
     4621 tgcacttgta tatcttggtg ttggtagaca tccacttata ggtttatttt ctggatatgc
     4681 agcagtctgt ctaggatttg ccgcaaacat tatgataagt gtcaatgata tacttgctgc
     4741 aagttttaca gttccagcag ctcaaatgat tgattctgga tttcaagcca atgcaacaat
     4801 gaacttattt tttatgatag catcaacttt tgttctaata cttttggcaa catgggtaac
     4861 agaaaaaatt gtagctccta gatttggtaa atatgaagga aatgcagaac tagatgttga
     4921 tggaagtctt agtgatgtag aaaagaaagg gcttaaaaaa gctggtataa gtatattaat
     4981 atatgtggct attatagtag ctcttagtgt aatcggtaaa aaaccattct tagctgaccc
     5041 agaaacagga gatttactat caaacaatgc tcctcttatg aaaggtatgg tacctataat
     5101 agcactagca tttttcatac ctggtgttgt atacggaaaa actataggta aaataaagaa
     5161 ggataaggat gtagtctcta taatggctag tgcaatgagt gatatgggtg gatatataat
     5221 actagcattt gctgcttcac aatttttgca attattcaca aatagtaatt taggtctgat
     5281 actagctgta aagggcggag aatttttaaa atcagcagga ataaatggac ctttattgtt
     5341 gataggattt attatattat catgttttat aaatttattt ataggtagtg catctgcaaa
     5401 gtgggctatt cttgcaccta tatttgtacc aatgtttatg atggttggat acaatccagc
     5461 acttactcag atggcttatc gtataggtga ttgtataaca aatccactta gcccattatt
     5521 tccgtacttc cctattatac tagcattcac aagaaaatac gataaagatg ctggtatggg
     5581 tactgtaatt gctaatatga taccatactc tatatcattc ttaatagtgt ggactatact
     5641 attaatattg tttatggtat ttaatattcc actaggacct ggaatacttc cttcatatag
     5701 catgtaattt atttaaatct ttctaaaagg agaaataatt tatgttagat aaaaatagac
     5761 ttataaataa ttttatggat atggttagaa ttgatagccc atctaatcaa gaattagaaa
     5821 tgtctaagtg gttggtaaac tacttaaaag aaagaaatat agacgccatt atagatgatg
     5881 caggtgaaaa atatggtggt aatacaggta atgtcattgc ctatataaaa ggtgaagaag
     5941 gaagtagacc actttgttta tgtgcacaca tggaccaagt tcaaccttgt ttaggtgtaa
     6001 aaccaatact agatggtaat gtagttagaa gtgatggaac tactacactt ggtgcagatg
     6061 ataaagctgg aatagctgct atacttgaag ctttagaaca tgttattaca gaaaaaatac
     6121 cacatagaga tatttatctt tgttttacaa tctgtgagga agccggtatg catggtgtca
     6181 aaaactttaa tcctgacaac ttgccttgta aagacatggt gattttagat tctggtggag
     6241 ctattggttc gatagcatac aaagctcctg ctcaacaatc tataaaaatc tcttttcatg
     6301 gaaagaaagc acatgctggt atagaacctg aaaaagggtt aaatgctata ttagtagcct
     6361 ctcatgcaat aagcaacatg cacataggca gaatagattc attgactact tcaaatatag
     6421 gtaaaataga aggtggtgga gctacaaaca tagtaactga taaggttaca ttgactgctg
     6481 aaattaggtc tcatatacct gaaactttag agtatgagct taatcatatg gaaaaatgtt
     6541 gtaaggatgc tgcatctaaa tttaatacta cttatacttt tgaacatagt atgtcttacc
     6601 ctagttttga acttagtaga gatagtcatg ttttcaagtt aagtgaagaa gcaataagac
     6661 aagttggtgt tgttccaaat ccaatggtaa ttggtggtgg aagtgacgca aatatattag
     6721 ctaatcttgg atataattgt gctatattaa gtctaggtat gtatgatgta cacacagtta
     6781 atgaatatgt aaatatagac gaattatatg atactgccaa aatagtttat catatgatta
     6841 aattataaat atcataatat aactctttaa attataaaaa taaaaaccta tgtatcttca
     6901 actttataag ttatatatta aagttgtttt acataggttt tagtatttta tataataatt
     6961 tgttttaaca tttttcataa taagcttcaa tattttgcat aataatttgt tctagcattt
     7021 ttaataacaa gctttagtat tttatattaa taatttattc tagtatttta cataatcatt
     7081 tgttctaatt tttttcataa taagctttaa tactttatat agtaatttgt tgtagaattt
     7141 tatacaatat aatgatttat acagttttat aaaatatctt aaaacagtat ttcagtaata
     7201 ttttttggtc ttcctccacc aatactagtc tcttttgcac atattcgacc taaacctgca
     7261 tttactattt tatttaatat acgtcttgca cttctttcac ttacatttag atactctgct
     7321 aactcttttg aatcataaat cttacttttt ctattctcat ttatagcaat aatttttgca
     7381 actgactcac aactcattcc agtcttttta gatatttcga ttaccttttc atcagatagc
     7441 atcaaagaat aatttaattc attttcggaa gctaaaggtc ctcttatgtt ttcatcttca
     7501 tctaccaaat atataccaat ttcttttgaa tctatggagt gtcttaatgc tttatgtgca
     7561 ttattttcag cctgatacgc agttactcca gtaccaattc ctatactaag agaaaagccc
     7621 atgtctttta tctctctctt tagattgaat agcttatcat aattatactc attatcagct
     7681 acccctttat gaacaaatac aacataatca tttcttccaa gtaaaaataa ggaaccttga
     7741 atacttctta catagtttac tacaagctta tccatatctg atttttttat catgtcagaa
     7801 taatagttat ctatcttctc tttataatct attaaactca atatttcaac agcaatttga
     7861 gaaaatcttg ccttacttag agcatattca ctctttacct tgtcataact ttctttgatt
     7921 aattgtatag tagcttgtaa acgaaataca ggatagcctc tttcttttaa ttcattatat
     7981 actgcaccaa aaccagttaa cattatatcc gtcttatttt cttcaaataa ttttatatgc
     8041 cattctacaa gttcagactc tcctacttcc aaagaaaatg gattacaaaa tacactcgtt
     8101 gctttaatat caaattcttt aagtgcatct tccacaacat atctatcaac tacatctata
     8161 ctgaatttat ctaattttat atttgcactt tttattgccc aaaatgcctt cattatactc
     8221 gtcccacttc tcggtacaaa tgaatgtggc ttacttatct catacttaga ttttataaat
     8281 tcataaactg cacatcctgt aaatataata gcatcacatt cgtcttcaca tttagaaata
     8341 acttctattg tatctactac tttttctctt acatatattt tagtctttaa atcactgtct
     8401 atctgtttta aatcatcctt tatcttaaac ccagagtctg aaggacctat aatacccact
     8461 atcataaact tcgctcctaa tccataaata ttatactttt taaaaattat atcctcacta
     8521 ttttctactt ctatttaatt attttgaact actccatcct acctacattt agaatggaat
     8581 aattagctta tttttgataa agtaacttct caaaatcaga acttggaata ggcttacaat
     8641 aataataacc ttgaattacc acacaatcta aactcactaa aaattcagct tgttcttttg
     8701 tctctacacc ttctgccact acatccatat ctaaatcttt tgccatggct atagtgtgct
     8761 ttaaaactat tttttctctg tctgtgattc cttcctcatt aaagaaacca caatctatct
     8821 ttaatgtatc aatattaata ttttttaaca tatttaatga agaatatgct gaaccaaagt
     8881 catccatatt taaaacaata ccatccattc taaggtcggc tattatctta gtaaataatt
     8941 ttatattttt aataaataaa ctttctgtaa gctctaattc taaaaaagat tttggtatac
     9001 tatatttatt tattaaactt aaaacaattt ctttaaaatg tggattatgc aaatgtaatc
     9061 ttgatacatt tacagagata ggaactggct cttcacctaa atctatccat tttctaatca
     9121 aagaacatac ttgttcccat acatacatat ctaatctgat aataaaccca ttcttctcaa
     9181 atagtgaaat aaatttaatt ggaggtatta tacctttttc tggatgaacc catctaacca
     9241 atgcttctgc accaacaatt ttatttgcat taagattata cttaggttgt aaatatacat
     9301 ggaattgccc attttcaagt gcgtattcca tttccctttc catctgtttt tcttcaagtg
     9361 catcatttct tatagtatca tcataaaata tatagttgtt gatactttgt ttctttactt
     9421 ttttgtgagc atatcctgcc atttcacacg catttgtaat tggaatatga ttatccaaaa
     9481 ttctataaac cccaaaacat ggtactaaca tatattcaaa tttttttcta tttaagctat
     9541 ttttaatctt ataaattact ttatgaatat ctctatcaga gtaactctca taaaaaatgc
     9601 aaaatacatc tgcatacata cgacaacata taccattaga accaatgttt ttttgaatca
     9661 actctgcaac atatattaaa gctttatctc ctcctctaac tccataaatg tcattaatta
     9721 ccttgaactt atctatatcc atcattataa ttgcataacg cttgtcagga tatcttttaa
     9781 gcattaaact tatttgttca caaaaacttt cataattgta tagtcctgtt aatgtatctt
     9841 tttggttttt atgtagttgc ataagataat ctatattttt tatagcaact aaaatatgtg
     9901 tttgtttctc acctaactta tttttactac taatctttaa catataaatt aatgcccaat
     9961 agtacttttt atctattcct aaacgtctat actcaaaatt tagttctaaa tcgtcttgct
    10021 tgctattatt aagactatca agagatttta gaaggtggtt atcattaaat aatattatat
    10081 catcaggatg aattaaatat ttttttatgt atgaaaaagt tttttcaaga ttttccttta
    10141 taggaaaact aaaaatattg ctctctgatt gataaattgg agtaattaaa ttcgtatcaa
    10201 tgcaaataga taaaattata tcatatgtat tataagctaa tttttcataa gctttagaat
    10261 acccttttat cattaaatca tcaaaataaa ccttatcaac taactcttta tgtttaaata
    10321 aaatattatt cgtcgaatca gctaagtcac taattacttt catttcatta tttctaagtt
    10381 tgttcttatt ataatacctc ttcttatctt ggtacattct ctcatcagca actttaatta
    10441 gcttatgtat atcaatatta tcgtcactcc aaatactgcc cactgctaca ctatatatca
    10501 tctcatcctt aaattcttca cttaaccaac tcactaattc ttcaaatagt tgttttgata
    10561 tatcttcaca taatacaaca aactcatctc ctccaatacg gtaaacatta taagaatgaa
    10621 agtatttttt tattattaca catgctgaca caatagcaga atcgccatta gcatgtccaa
    10681 agttatcatt aatatctttt aacccattta tatctacaaa tacaactcct atggatttaa
    10741 cttttccttt ttgaaaatta tctatatact taatatattt atttctatta tatgcaccag
    10801 tcaagaagtc atagtagctc atataattta atttattttc tacttttctt tttttcatct
    10861 cagttaataa aaaataagat agggatgtca aaagagaggt atctccaaca gctttatgtg
    10921 gattatcaac tccaataaac cctactactt tatcatcttg ttttagtgaa gatgtcatta
    10981 aactttcaat tttttgaggt tttaaaattt catattctat tggctgttct tcttttatat
    11041 tttctatatc atcaattatt acaaaacctt tttgttcgaa taataaaaac caattcttta
    11101 attcattaat atcaagattt gttagtgcct gtttttgttc atcaacacct tcattgcacc
    11161 attcatatgt attagttact tttgttctat cttctgatac ttcaaaaaca tatgctctat
    11221 cagctttatg aaatttacca atattggtta atacaagatt tacagcatca gaaaaattag
    11281 attctgttat aagatgttta atacatccaa ctaataactc ctttatctct aatttttctg
    11341 ctattgactt acttatatct tccttttctg taatatcaag agctatttct aatcgtgcat
    11401 gtttgccctc ccatttaatt aacttgtcct ttaatataaa atgtctattt gttcgagtat
    11461 tcgtattttc ccatatgtaa tattcatctt cttttaaaat agagtttgta caaaattcgc
    11521 agggagaatc tttcccctgt aatgctttat aacattttaa atgggatata tcattaagtc
    11581 ccattgcctt tttcccaatt tcattaatat ataaaagttc atatgtatct aaagaagaaa
    11641 catatacaat ttcatccaac tcatctatta aatttattac atccatttat atatatctcc
    11701 ttttattcat tttccagatt gaatttttaa agcttttaaa attttgctat atatttggca
    11761 atgatattat acttctttat gttttaaaat agtaccttaa tgaggttcta ttttttagta
    11821 aatagaatta ttattgtgtc aatcgtacaa tagagttttg taatacttaa tttaatatat
    11881 ttataattaa ctatcttatt atgtatttac aattaattct catataaatg acaaatctcc
    11941 acctcaatta agtattccaa tcatatatga ggataatact tcattcattt aaaaatataa
    12001 aactattata tattaaaatt tcttcaataa taaattattt agattgttac tttgatatat
    12061 tatataatat cacaaaggct gatataaaaa gacaaaaagg gcaatttttt tatatcaaaa
    12121 aaaatgttaa attgatatta tccatatata aattgatatt atccatatag gtgctatatt
    12181 taacaattct acttacttcc cttacactta ttagcatata gttattaaga gtttgttgca
    12241 aaacaacact tagcacactt taaaaagtgt ggtctaataa gtaatcaacg aagaagacat
    12301 aataaagcat aaaataggat atacttttcc acgtcatcaa aattgaggat tttattatta
    12361 atatatgata aaatatgctt aataaattaa attttaatgg gagaatagtt atgaaaatat
    12421 ttagtttctt gttgtttgta gtgggaatat gtttttgtat tgctggattt atggggcatt
    12481 cctcagcttt ttctggtgca acattgagtt tttttgcaac tacaatgata gctgtaagac
    12541 aagtcaaaac taagaaaaaa tgaaataggc aaatcctaac tttataagtt aatattacat
    12601 aagtaattag tctaaaatta gatacttttt ctattacaaa aatacatcat ccactttgta
    12661 ttctcttaaa aaaaataaaa tagctatgta aattcaagat tagatagact aactctctat
    12721 ccatttcatt gtaaaatata catagctatt tgtattcgat ttaaaatact tagattttct
    12781 tacataacaa ataatttcta tctgattatt actcatgttc atttattaac cactcagcaa
    12841 ctaatcattt agtatctatt catccgccat acggtaacct accccaacct cagtcaaaat
    12901 atattctggc tgagcaggat tcttttcaat tttccttctt atacttgcca taaaaactct
    12961 aagcgactga ttttcacttt catagtaatt tccccaaact ttatctatta taaacttatg
    13021 ggttaagact cttcctgaat acttagacat caactcaata atcttatatt cgattggagt
    13081 taaatgtatt tcttcaccat taattgacac aatatgattt tcataatcta tctttaggtt
    13141 tttaacttca aaagactttg gagcatcatt atttatatta tcctgttgag ctttatttct
    13201 aagagccaca cgtactctag ccaaaagctc tgttacacta aaaggtttag ttaaataatc
    13261 gtctgctcct ccatctaaag cttccacctt atctctctca tgctctctcg ctgaaacaac
    13321 aattatttta ttagtctttg cacatgccct aactcgtcct attacctcaa tcccatcaat
    13381 atctggtagt cctaaatcca aaattatcaa atctgggtca agagaatgta taagtgatat
    13441 agctgttcct ccattatctg tctctacaca cctataattt tgagtttcaa gagatacttt
    13501 tataaattta caaattggct tatcatcttc tacaattaga actaatggct ttatttccac
    13561 ttgtctttcc cctcctcatc atacataggt atgctaaatc taaatgtagc tcctccagaa
    13621 ggattattaa aggcgacaat ttctccacca tgagcttcta ctatagattt acatatctcc
    13681 aatccaagac caatacctct tctttcaaga tttctagact tattcttagt ataaaaccta
    13741 ttaaaaatat tcccgatatc ttcatttggt attccttttc cattatctgc aacttcaaat
    13801 agtacttgat ttgactcttt ttttacagtt agttctatct tagaattttt aggtgtatgc
    13861 cttatagcat tatcaattag atttactaac acttgttcta ttaataatac atccacatga
    13921 acgattatta tctcatctgg aatatttgtt tttatgtctc tactattggc aaactttttg
    13981 actcttaata tagattcagc tattatttca tcaacaacct ctggtcgttt ttcaatatcc
    14041 aattttcctt catctattct ggtcatacta agtatattct caacagaatg agttagccaa
    14101 ctcgtatctt catatatatt tttcaataat tctactctag tttctttatc tatcacatca
    14161 tcattttcaa gtattgttga agatgaaccc aatatacttg taagtggagt tcttaaatca
    14221 tgagaaattg acctaagcaa gtttcctctt aacctctctc tctctgcctc aaggtttgct
    14281 tttttatttt tctcaaataa attttctcta tcaattgcca acgcaacctg tgttgaaact
    14341 gcttctaata gtatcttttc attttctgta ataatatcta tcttaaaaca cgcaacacct
    14401 ataacaccaa gaacatttga ttcttgacct tttataggga aataatatgc actgctgttt
    14461 ttacaatatt ttgtcccaat tccaactggg ttacctgtcc taaaagcttc ttttatagct
    14521 tccttttcta atactgaatg gaatatattc tcacttccat cattgttaca aatataaata
    14581 cttggcttac ctagattatt attctcatct gcaatagtca ctattacagt tctatttaat
    14641 atatttacta aattagaccc acaaaattca ataatttgat ttttattttt tgctttaagc
    14701 aacgttttgt tattctcata gagcaatctt atcatttttt ctctcttatg agaaagtctc
    14761 acttctcttt ttatcctaga agttaaagta cttgttataa ttgctgccat tagcataata
    14821 caaaaggtca taggataatc ttttctataa gctaaaaaag tatataatgg atgcgtaaag
    14881 aaaaaattaa atgatagcac tcctattact gaagaaagta ttccaaaaaa atatccatag
    14941 gttatacttg tagcaaatag tacacctaat atgtatacta gtataatatt agattcatta
    15001 aaacctacct tttcaaaaac tactgatatt ccagtagcaa ttgtcataac caatatagtt
    15061 tttatgaagt ccttaataat tatcttttta ctgagatagc catcttcatc ataatcatac
    15121 tcttttaagt cttcatgttg tagtcgttcc tttttcattt actacaattc ctccttattg
    15181 ttactgttta aattggctct attttatata atacaatttt atcataataa attcaatcaa
    15241 tacatttaat taatgtactt aaattactaa aaggtgctgt cttaaaatga aaataaatct
    15301 ctatttcaaa acagcaccta tatatttaaa ttactaattt agtttttaca tacttaaatc
    15361 aatagtttta ttcaatttca taaattataa tacttcaatt ataaaacatt tctatccaac
    15421 aagcataaat ataaagtggg cacatatacc tgcacataaa ccacctatag tatgcgaaag
    15481 tatcgcttta ccagccaatg gtcttgcatc taaagcatcc atcataccta tgtgtgtact
    15541 taaatatcca ctccaacaca tacccattgc tgtgaatact gctatatcat taggagttat
    15601 agcgcctgat tttataaact ctggtactaa agatattgct gctccaacag ctcctaatgc
    15661 agttacaggg aatgctattg cctctggtga tgtgaatcca aataatggtt ctattatgaa
    15721 gctaatctta tcaccaatag caggtaataa ttttatacct tcatatgcag ctcctgtgta
    15781 aacagcttga cctgtagcag ggtctgtaga tggtccaaaa gttaataaca taactaatgt
    15841 acaaactact aaaactcctg gtattatagc catacccatt tcaacaccta acttaccacc
    15901 ttcaagtata gcatccaatg ttctttggaa tacattacca tcccttattt ctctaaactc
    15961 ttcaagtttt gcacctttat catttatttg cttttcagta tcatttttag ggtcataatt
    16021 ataatacttt ttagtaaaag tcaacataat tctaacacta ataatactac ctattattgc
    16081 cccaacatta cctattatag ctggtagtac atattcttta ccttgagcta tcataaaagt
    16141 tgttacaata agtcccatac caaaggctgt tcctaaatta cataaagcag gtacttgata
    16201 cttcttaaaa tattgtgtaa atgttttgtc tttagcaaat ggtataatag ctggattatc
    16261 agataaatat gttgcaacta ctcctgctat actagcacca ggtaagcccc atataggtct
    16321 cataagaccc ttaaatatct tattaactaa agatattact ccaaactctg aaagcaatgc
    16381 actaagtgca cctgcaagaa ctgccatagc cattattaaa aatactgtgt ctaataaaag
    16441 cgcatgtgct gtactcatta ttactttaaa catcattcct gcacccatga tgcttcctac
    16501 atatccaaat cctaccgcca atagtacaat aaatacgaag gtttccatac ttactgcctt
    16561 gactcttctc ttatccgaca gcttttccat cttgaaatct tccccctttt aattttaaat
    16621 taaggttcct aacattggta caaatttttt ggtacaaact gagttctaaa acttacactc
    16681 gtaatgcaag ttgtcagttt tttgaaataa aaaaaaatta cgtttataga ataagaatat
    16741 cctctttaat ttggatactc ttattctatt ttatgttatt atattatcat ccttttgttt
    16801 aaaagtcaat tttttctatt tttgttgttt aattttattt atactctttt tatatttttc
    16861 attttctttt atctttgtaa gtactccata taagttgact tttctaaata cagtagttat
    16921 aaataccaat cctaatgcaa ttgaacttgc acacgataat gtactcaccc catattctac
    16981 agcttttata taattattgg tcaaaacaga atacatagta ttatatattc cataacctgg
    17041 cactaatatt attaaacagc aaacacttaa tgtagttgct ggagtcatat aaattcttgc
    17101 acataactca caatatatcg caaaacaaac tgtagctata aaaaaacata agttttctgt
    17161 tactcccata tttaaaagaa atttatacga aaaccaagct atacttccac cgattcctgc
    17221 tagtattaag ttttttcctt tcatattaaa tagcactcca aagcttaagg cagtaaaaaa
    17281 agctgctatt acttctataa acaaatgcac ttatatcccc cctaacttga agtacaatcc
    17341 aagtaccaag aacattccta ctgctataga tacacctaca aaaaatactt ctgctaattt
    17401 tgcaagacca gaaatcaact gtccatctaa caaatctctt aatgcattag tcaaagcaag
    17461 cccgggaact aagagcatta ttgctcctgc tattaaatgg tctaacgtct ttatgaatcc
    17521 gagctttaaa attaaaattg aacaagaagc tataataact gctccaattg catttacaaa
    17581 aaagtcatta agggaagatt tacttaaata cataataaca aacttagtca gaatcccaac
    17641 agtaaatgca gctataaatt cttccacatt tcctccaaat aatatcgaaa aagttgctgc
    17701 tgcaatacct gcaaaaaata ttgttattaa atccgagtaa tagttatctt cacttatttc
    17761 atctaaaatt ttctcacata attcaatatc taaattattt atacttattg ctcttgataa
    17821 tgagttaact tcatgaacca agtttaggtc tactcttctt gagcttattc ttttaattgc
    17881 tgaatgtaat ttcccatcta caaatacaga tgccattaca gctgttggag ttgcaaatac
    17941 atctacgtgg tctattccaa aagattgaca tattctagct atcgtttctt caactctata
    18001 ggtttctcct ccactttgca acatagcttt acctgcattt gaggaaaagt ttaaaattct
    18061 atctatatcc acaaaacata tctcccattc ttttatagta ttattcatat tttaatacaa
    18121 gatattattt atttaaacta ttttaaataa ttcatatcca acatttacat tttatgaaaa
    18181 aacttgtttt ttatgcttgt caaaatcaaa ttttaagctt gtttacacca taaaatggct
    18241 gaattaatat ttgacaaaca ctttcaacca tttatataac cttctaaatt gtataacttt
    18301 ttactacaaa taaaattata tctcttttaa ttctttctct tgagtattta gcttatagta
    18361 acttcatatt tatatatgta caatataaaa cattattttt atgatacaaa atataatttg
    18421 ta
//
LOCUS       DAMJWU010000072        17664 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMJWU010000072 DAMJWU010000000
VERSION     DAMJWU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.073"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(105..725)
                     /locus_tag="PC460_002578"
     CDS             complement(105..725)
                     /locus_tag="PC460_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2601458.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(1039..3177)
                     /gene="vanT"
                     /locus_tag="PC460_002579"
     CDS             complement(1039..3177)
                     /gene="vanT"
                     /locus_tag="PC460_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2601459.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(3195..4001)
                     /locus_tag="PC460_002580"
     CDS             complement(3195..4001)
                     /locus_tag="PC460_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2601460.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3998..5098)
                     /gene="vanG"
                     /locus_tag="PC460_002581"
     CDS             complement(3998..5098)
                     /gene="vanG"
                     /locus_tag="PC460_002581"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2601461.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(5359..6441)
                     /locus_tag="PC460_002582"
     CDS             complement(5359..6441)
                     /locus_tag="PC460_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2601462.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(6491..7192)
                     /locus_tag="PC460_002583"
     CDS             complement(6491..7192)
                     /locus_tag="PC460_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2601463.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(7626..10157)
                     /locus_tag="PC460_002584"
     CDS             complement(7626..10157)
                     /locus_tag="PC460_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2601464.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(10265..10882)
                     /locus_tag="PC460_002585"
     CDS             complement(10265..10882)
                     /locus_tag="PC460_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2601465.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(11241..12125)
                     /locus_tag="PC460_002586"
     CDS             complement(11241..12125)
                     /locus_tag="PC460_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2601466.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(12318..13568)
                     /locus_tag="PC460_002587"
     CDS             complement(12318..13568)
                     /locus_tag="PC460_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2601467.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNIDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(13790..14554)
                     /locus_tag="PC460_002588"
     CDS             complement(13790..14554)
                     /locus_tag="PC460_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601468.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(14548..15273)
                     /locus_tag="PC460_002589"
     CDS             complement(14548..15273)
                     /locus_tag="PC460_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601469.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(15275..15643)
                     /locus_tag="PC460_002590"
     CDS             complement(15275..15643)
                     /locus_tag="PC460_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2601470.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(15819..17363)
                     /locus_tag="PC460_002591"
     CDS             complement(15819..17363)
                     /locus_tag="PC460_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2601471.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKRNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         6059 a         3096 c         1770 g         6739 t
ORIGIN      
        1 tttacaaaag aactaaaaat tttgtacctt aatacaaaaa ataaaacgta tcttaaaata
       61 atttagtact aaatcatttt aagatacgtt ttatttcgta caagctaaat cattttatca
      121 tctttatcat tgtgatgttt tttatgatgg aaatcataat tatgattttc aagattttta
      181 tttactctat ttccaatctt ccatgacaaa aacaccaata atataaatga tgctatcttt
      241 aaaggacttg ttataaaacc ttctatatca cttgccacgt aacttataac tataaacatt
      301 acaaattttc ctgccaacat tgctggcaca aaatctttta aatctttttt accaaaagct
      361 gatgcgatag taactaaaca accgggcata aatggacagg catacgctat aaataataac
      421 ttaaatccct gtttatccat ccaacttatt gctttttcag ttttactatt tctcaactta
      481 ttaaaaagct tactatcatt aaatctgctt attaataaga ataatgaagt tgtcccaagt
      541 cctgagccaa tccatgataa caaacatcca ataaataatc cttgtattgc agcatttgct
      601 gtaactatag caacaagtgg taaggctggt ataaagcttt ctataaatgc acttaataag
      661 ccgattattg tggctaacac ccaataattt tgagtcaaat caagtagttc ttgtatatac
      721 tgcatattgc ttttcccctt ttttaacttt aactattatt atatactcta tatgtttatt
      781 atataccaat ctcaaaaaat gaatctaata ttaaaaagta aataatctat ataagtttac
      841 agttaaggta taattatttt attatatatt ataacaaaaa aggtgatgtt tcaaaataaa
      901 aatctgaaac atcacctttt tataacttaa attgtcatgt aagttataaa aaatatatat
      961 tagacataat attactaaaa atgtctagtc agagtaaaat aaattacaca gtacaacaaa
     1021 tattactatt taattgagtt acttctttat aacatttaac cttcttccta ctctgctcaa
     1081 taattcattc gatatactac atgagttatt tgcaacttca gaaacagata attcagataa
     1141 attatctctt cctattatta ttgccgtatt accaacttct acattaggta tatctgtaat
     1201 atcaacagca agttggtcca tgcaaattcg acccactatt gctgctctac aaccatttat
     1261 tattacgtaa ctttttccac aggataaatt tctaggtata ccatcggcat agccaataga
     1321 taatatagca attacgctat ctctgcttgc aataaagact cttccatatc caaaactatc
     1381 atctttttgt atttttctaa taagaattac ttgtgatttt aaggataaaa caggtcgtaa
     1441 atcaagttgc aatttagtcg tatcatttgg aaaacttaat atcccgtata atgcaattcc
     1501 aattcttaca taattacact taagattggg ataattaaaa aaaccataac tactttgaat
     1561 atgtacttta ggaattttta tacctttttt taacagaaaa tcaattaact tataaaaaca
     1621 ttcaatttgt ttatttgtaa agtctacatc ttcatctttc agactatctg aaacacacaa
     1681 gtgtgtaaat atgccacata tatctaagta ttttaattta aatgccttta aaattgacct
     1741 cacatcatta acatcaaaac ccagtctgtg catacctgta tcaattttaa tgtgtgattt
     1801 tatgttgtat ttctgtttgt ttaaagagat agcatgatta aggtcaataa cactctgtgt
     1861 aagattgtat ttatgaagtt cctttgccct tattgggtta gtatatccaa gaatcaatat
     1921 ttctcctagt attccatatc gacgtaaatc aatcccctca tctattgttg ctactgcaaa
     1981 tgcttttaca cctatttggt ttgcacaagt cgctacttca aaagcactat gaccataggc
     2041 attagctttt acaactgcca tcaattcaca atcttctggc atagcttttt gtatcatctt
     2101 tacattatgt tccaaattat ttaaatcaat ttcaatccaa gctctattgg tatttacctt
     2161 atgttctcct ttcaagtctt tttttagagg tttaagaatc aatataataa ttatagaagc
     2221 tataaaagat gctatactta ccactatata atgaataagg ctattgtcaa ccaagacagt
     2281 ttgtattccg aatattttag aaaacatacg aataacaaca atcatcatag gatgtataat
     2341 ataaataatt aatgatgatg ttcgcaataa cctagctcga gaacctttca aaaatgttaa
     2401 ggctgtaaat aagaaataca tacatggcaa taacataata tacatgctat catgacgctg
     2461 tacccctagt ttatgtaaaa acattccctc acataacata agtaagaatg atataagaaa
     2521 tccaatcaag ctatttttag ttgacatata attagattta ttagatatta ttccaccaag
     2581 tatgaaaaat acaggtgcaa aaaatatacc atttcgtgta taatctgata cttcaaaaat
     2641 atacccatat aaattcttta atagtggtat tttttctaaa aaaccataat aactatcacc
     2701 aaacatacca attacataaa taacaatagt gattactagt gagtacttta aacctagttt
     2761 ttttacaaga aagtatgcaa tacttgcacc aataattgat gcaggaagat accataaatg
     2821 atataaagtc ccatcaaata caatgtcctt aataatattg ggcactaaat tattcatgga
     2881 aaaatatcca gtgtaaatat ttaagggaat ataaattaca atagctatcc cgtaaataaa
     2941 taatacattt tttagaaaag atttcagttt attacaatta taattatatg ttgaaattag
     3001 aaaaaagcct gatagcataa aaaagaatgg cacagcaata cgagcaatta tactagttaa
     3061 cacaaagtca gccattttac tatatgataa taatggagat gtatgaattg caattataag
     3121 taatgcagct ataatcctaa aataatcaag ccctgcataa gatttattcc taatcattct
     3181 tgttcctcca tttcttagtc ctttctattt ctccaaaatg tcacaataaa accatcctta
     3241 ttgttacccg atatttgata tacacattgt tcaggtatag agattctcac ttcttcttta
     3301 gtatgaatag gcacataaaa gatttctata attttttctt tatcttctat ttttaaacaa
     3361 tttgtatacg aaaatctctt aatatattct atatattctt caagagcgag attcatttta
     3421 ttgataatct cagaatgcgg atatcccaca taacgaaaat gccatggttc ttgagatatt
     3481 cctgttattt cttctttttt acactgataa cgctctataa aaccataatg tagtgctgcc
     3541 ttacgaaact cattacaaat accattataa ggaaattcag ggcagataaa atctataact
     3601 tctttcttta accctaaatc aatagcaagc cctgtctgat gttcactgtg atttggaagt
     3661 gctacaaact ttttagtaaa gtcttttcca ttgtcacgca gagaacttga atatatatct
     3721 tgttgttcct ctgcagtacg atatccactt acaggtacta tttcatttat tccacatata
     3781 ctttttaaaa tattttgtaa aatgtttgta gcttcacgtt tcataagaat acttggaaac
     3841 cttgtatcta caggaattaa atctatatct tcagatgttt taactggtaa aaaagcattt
     3901 actagaatta gattcccatc ataaacttta tctttagtca ataagatagt atttgtcatt
     3961 ttatcttctt tatttttatc taataaaata ttattcatca ttttatgtac acctcaatta
     4021 tcttgtccaa tatttcttca aaagacagtc caataccttt catcatattt ggataacgac
     4081 tatgagaagt aaagccagga attgtattga cttcattaaa aataatttca ccagatggag
     4141 ttaaaaacat atctactcta gcaaatccag aacatccaag agttctataa ataatttttg
     4201 ctgtctcttg tattcttttc tcacaatcca aatcaattct agctggcata tgaatttttg
     4261 aacttttcaa tgtatatttt tctgtataat caaaaaatcc ttttgacaat tctatttcat
     4321 ctactcttcc aacaagtaag tcattgtttc ctaaaactgc acaccctacc tcaaaaccat
     4381 caatattttc ctcaattatt acttcattat catgttcaaa agccaagtct actgctactt
     4441 gtaactcttc ttttttataa attttactaa taccaaatga tgaacctgca cgcacaggtt
     4501 ttataaataa tggataagtt aagtcttttg acagttcagc cacattaaat ttatctgagt
     4561 attttagtaa tattgatttt ggtactttaa taccttctga atgaacaagt ttatgtgatt
     4621 tgtctttgtc catacataat gctgaagaaa gagtaccaca tccaataacc tgtatacctg
     4681 ccaattcaaa taacccctgc actgttccat cttctccatt ttttccatgc agtacaggaa
     4741 acacaaaatc aagtttagtg tatttaatat cattttcaca tgattcaata gcactatttg
     4801 gaatatgatt ctgagaaaca gcatcaggaa ttaaaaattc aataatacca tttttgacac
     4861 ggttctgcga aacaataact ggaattatat cagtatccct ttcaaaccaa gtgttattct
     4921 ggatattatt ataatctcca atataatgat accaattacc ctcccttgta attccaattg
     4981 gtataatatc atatttttcc gtattcaaat tctcaagtac tgcaaaagct gattgtaatg
     5041 atacatcata ttctgttgag tttccaccaa atataactgc aattttttta ttattcatct
     5101 ttgattctcc tttgttgatt tatttatttt tatacaatct acagaaacat gttaaaatac
     5161 tttttctatt tcaaatccaa tcaaccttca cttacaattg catctgttcc acataatata
     5221 ttttcacatt tttctattga tataattact gtatcaaaat aaaagcactc ttgttttgat
     5281 acagtaatta tatcaataac aagagtgctc tttatttatt tatcctaaag aatttcctat
     5341 ttttttatac ttaaattctt acaaatttcc tacaatcgga ataataacct taaaggtaat
     5401 tatattgttc tcgctatgag ctgttattgt cccgttatga agctccacta tttctttagc
     5461 tatagcaagt cccaacccag ctctaccact gtttgaatta cgagatgtat ccaacctgta
     5521 aaattgttca aaaatacgct caagtttttc ctctggaatc gtattgctat gatttataaa
     5581 tttaagcttt atataatcat cctcctgtac agctgttatt tcaatagtag tatcttcaaa
     5641 actataataa acagcatttc gtaataagtt atcaaataca cgttgcattt tattaacatc
     5701 acaccttatc atcatatttg gtgtaacatt cagtgtatat tttaaattct tactcatcaa
     5761 agtaggttta aactcatatg ttagttgttc aagcatacga gttaagctta ctgtactgta
     5821 ttcaagtgat atatttgata aattaaacct tgtaatttca aagaattcat taattaaatc
     5881 ttcaagacgc tctgccttat ccaacgaaat agataaatat ttttttcgca gttcttcaga
     5941 aatctgactt tcatctcgaa gtagtgtcag ataccctata actgatgtaa gtggtgtttt
     6001 taaatcatgt gcaagataaa caataagttc attcttacgt tgttctgcca tttgtgtctc
     6061 aaatttacgt tgttcaagta tatgtttaat tgagtttatt ttcttttcta ttgccaataa
     6121 ctctggagat aaagccactt ctccaacatc ttctttgata agagagtcaa taccttgatt
     6181 tatttcacta aaatattttg taaaccaatt taaataaaca cggaatatta taaaaaacac
     6241 aatcaccata gctagtatca tgaagagctc catactatta cggaaaatct gctgatataa
     6301 attcaatgca gctttatatt ccatacgaaa agcattttca aagaatgata ctatccagat
     6361 agaaaactta ccatacaata taaaagaata taaaatccat atgaaaataa tggctataaa
     6421 tgccattaaa gctgttctca taaaaatttt atttctcact tgagtaaagt caggattgac
     6481 tttaatattt ttacttttca attgtataac ctactcccca tacagttttt ataaatttcg
     6541 ggtttttaga aggttcatga attttttcac gtaatcgccc tatatgagcc ataactgtgt
     6601 tattattatc aagatatttt tctccccata cagcttcaaa caattcttca gaaggaacca
     6661 cttttccttg atgttcacat aaataccaaa gaatagaaaa ttcaatcggt gttaatgcta
     6721 cttctttccc aaataaacta catttatgtg tttctttatt gataatcaat cctttaatat
     6781 catactcatt aacaattatc gactgttgtt cataagaatt attatatcgc atatatcttc
     6841 ttaattgtgt ctttactctt gcaacaactt ccaaagggtt aaatggcttt gttatataat
     6901 catcagcacc tatggttaaa cccattatct tgtctatatc ttcaatttta gctgttaaca
     6961 taatgatagg ataatagtat ttttctcgaa ttttctggca aatatgaaat ccatcaatat
     7021 caggaagcat aatatctaaa atagccatat ccaaattttt agattcaata cattctagag
     7081 cttccttacc tgtataaaac ttgtatactg taaaaccttc attttttaaa tatacttcaa
     7141 ttaaatctgc tatttctttt tcatcatcca caatcaaaat attttcgttc atagattatt
     7201 tttcactctc cttattatcg aggtcatcat ttataatatt ggtaaattta catatcaaat
     7261 aattacaagc atatttgtaa ctaattttta taagaaaaga actactacat cagaatgtgt
     7321 tgatgctact attggaacac aaaaactgag attaattgtt attataaaaa ctcccctaac
     7381 acgtccttat tatcgcataa caggtattaa ctttcaataa aaatatctat cagtcaaaca
     7441 caattcaatt tattatacca gagacaatcc atataaaaaa caattaaacc tatgtatgta
     7501 accacagaca ttactaaaat ttccatgtta ttttatataa aaattcaaaa aaggagttga
     7561 atttaaaatt tgattagaaa aaaatcaatt ttaagacaac tcctttttat tttaatgtat
     7621 ttaaattata gtatatcagc cttcataata tcctcaaaat cacctttttc agatattaat
     7681 cttatcaatt taacagtaca gcttaaatca tttataagta tagctccaac taatgaacca
     7741 tctttcataa ataatttttt atatattctt tgaccttcat cttttgatga aatttcttta
     7801 tcaaaatttt gtatattacc aatactaaat acttttacat tcattgcatc taatgaaact
     7861 ggatatactt cactttcaaa tgtcaaatta tctccacttg catttgcccc tgctattttt
     7921 cccatttcta ctgaagatgg ccatattgct aaactaatat ttcccacttg tgcaacatct
     7981 ccacaagcat atatatcttt aacatttgtc tgtaatgtct tatctacaac aatacctctg
     8041 tcaattttta cagatgtatc ctgaaccatt tgagtgtttg ctcttacacc aattgaaaat
     8101 actaccatat cacagtcaac actatctcca tctttaaata caactttttg agcttttcca
     8161 tcaccttcaa taccatcaac tgctacaccc aatcttacat ctattcctgt atctcttaca
     8221 cattgttcta atattttact gccttcttta tcaagttgtt ttgatagtat gctatccatg
     8281 gcttcaacta ctactacttc taatcctttt agtctaaatt cccatgcagc ttcaagtccc
     8341 aataaaccgc caccaatcac tacaactttc ttagattttt ctagtgcttc ttttacacta
     8401 tataaatctt ttatatttct aagagtaaac acattttcta aatcatgacc cttaattggt
     8461 ggtataaagt ttctacttcc tgtagctatt acaagcttat caaatttaat agctccatca
     8521 ttcactataa tagttttatt cacttcatct aatttatcta cattcgttcc taatattact
     8581 actatattat ttttatcata ccaatcttta tcttccatgt agaaatctga accaagttct
     8641 tcatttatac catcagaaag tgcaggtcta tagtaagtca actcatcttc attagaaatc
     8701 atagttatct tacaagtttt atttcttttt cttatagcat ctgctgcata aaaaccagct
     8761 gctccatttc ctactattac gaaatattca tcagtatctt ttctaaaagt aacttcatct
     8821 tcttcaactt ctataaattg gtcttcctta gctccacata cagggcatac actaggagca
     8881 ctatcacctt caaaaacttc attacaaatt acacatttaa atttcttagt tttcttttta
     8941 cttccaaaag tagaatttaa cttatctagg aatttacctg tataagaatg taatttatca
     9001 atctcttcat ttgatggttt aaagttaaca gcaaaaggtt ttactgctgt cattctaagc
     9061 tgacttattc tctccatagc attttctata ccttctccac tccatccata tgaaccaaat
     9121 actgaagcaa cttttccacc atgaagaact gggtttaacg atattaatat atcccatata
     9181 ggctttaacg catctcctaa tattgttggt gttccaaata atacaccttg tgatactgct
     9241 atttttgcca aaatatcttc ttgcttatgc tctattacat caaataaatt tactttatag
     9301 ttgctatttt tttcaatata tgcttttata gcttctgcca taatctttgt atatccatgt
     9361 gcagaaacat aacaaattgt aacttctttt tctaacttta tttgttcatt tacactccag
     9421 ttgtaataaa ggtctataat ttttctagga ttttcagtta atactggacc atgacctgga
     9481 catacagtat ctatatctaa atcttttatc ttttctatgg cagttaccat agatggttta
     9541 aatggtccca ttatacaatc ataatagtat ctcaaagcat ccaagtaatc cttttcttca
     9601 tcttcatcaa gtgtatttac tatcttatca ttactatagt gactaccaaa tgaatcacat
     9661 gttacaagag ttttatcctc ttttatatat gtgtatatgg tatctggcca atgaagcata
     9721 ggtacagaga aaaattctaa agttttgtcc ccaattgata atgtatctcc atctgtaact
     9781 gcaacatatt caaagtcttt gtttacgatt tccttaagat atttaattgc tgtttctgaa
     9841 gctactactt ttgcattttt agctaaatcc aaaagctttt caacgcttcc tgcatggtct
     9901 ggttcagtat gacttacaac aatatagtct attttttcaa aatctatatt taaatcattt
     9961 aatctttcaa tataattgtc aaaatgctta tctttaactg tctcaaataa aactgttttt
    10021 tctgttcctt ttaatacata tgaattataa gtagttccat aaggtgtata cataattatg
    10081 tcaaatactc ttaaatcagg gtcaagcgaa cctacccaat ataaattttc ttttattttt
    10141 agcgatttca atgacatacc gtactcctcc taaaatagtt atttagtctc tttataccct
    10201 acttgttata ttttaaacaa attttattca aaaaagaaat tattttaaag atagtttaaa
    10261 tcttctaatc ttctttttct accttcaata cagttccctt tttagaatct acttttatgt
    10321 catattcatt tttatcttta tctattctta ttttataaaa agtaacatct aaatctttat
    10381 ctaaactcca ctctttaacc attccatttt gttcttttaa tgcaatttcc atagcttgtt
    10441 gaggtgtcat tatgtcatta aaatctaatt tttcttttct agcgactcca tttaaatctt
    10501 ctgaatccaa cttttctgtt ttatcttcta atacatcttt tgtattagca tcaattttca
    10561 tcttatattc attattatca tctacacctt ccacagtata aacaaattta tttaaatctc
    10621 tctcaagact taaatctact atatcagcat ctttgtattt gtctttaaat atgtttattg
    10681 catctgtgta tggcacttta aaatcttgtt ccattaaatt ttcattattt ttttcatctt
    10741 ttaaagcgtt atttgataca tttgcctcct tttttgtatt atcttgtgct tgttctgaat
    10801 ccttagttgg actcccatta catgcagtta ataaacctgc tataagtata cctaccacta
    10861 ataaatacat ttttttgcac ataaaaaccc tccttaaata attaatttat tctatattat
    10921 gccctctaaa ccctttattt acccttttat attaaaattc ctcctatttt agtagtttta
    10981 aaactttata ctcattaaga ttaaatctac ataaaaataa tattgtctca aaatagaaac
    11041 agacctcttt tttattgttg aaataataaa aatatatttg atttcataca aaaagagtgc
    11101 ctcatgaact aaaaagttca ttttgagaca ctcccatttt ttatattttc acttacaaaa
    11161 taacaggact tattaatttt tcacaaatct tataaatata tctatataat attcttaaat
    11221 ttaaacacta cataaaaaaa ttaaatttta tttaattctt ttaccgttaa atccatttta
    11281 ggatatggaa tcgtaattcc ttcttcgtca aacctaactt taacctcttc tagtaaatca
    11341 agataaattg tccaataatc ttctgtcttg caccaaactc tagttgcaaa tttaatagca
    11401 ttatctccat gttctactac tcctataaat ggttctggtt catttattat taatttatga
    11461 ttattaacaa tatctttaag tactcttcta acatgtagta tatcatcttc ataccctaca
    11521 ctaaaagtca aatcaactcg tctagtattc tttgctgaat agtttattag actatcattt
    11581 gatacagaac cattaggtat aagtatctgt ttattatctg gagtaaccaa ttgtgtataa
    11641 aaaagtccta tttgttcaac cgttcctcca tggcctgctg cttctacgta atctcctact
    11701 ttaaaaggtc taatcaatag tataataaat cctccagcaa aatttgatag actaccttgt
    11761 aaagccaaac ctattgctac ccctgctgat gctatcacag cagcaaaact ggacaaactc
    11821 attccccaat attccagaac aatgattata acggcaactt taagtacact caaaagaaga
    11881 gacttcaaaa accttcttaa agttaaatca atatctcttt tactaagtaa taacataaca
    11941 tggtttacaa atttttttat tattttaaat ccaatagaaa gtataagtat gcctattata
    12001 agttttactc cgcttgtagt agcccattct aatagacttt ccattatacc tcccatatcc
    12061 atcttattaa tatctaaatt atttaaatct aacttgctta tatcatctat tttcgtcgta
    12121 tccataaaaa actccttacc ttatatttta gaaccaatat tatagtttaa agttcttatt
    12181 ttctatttaa tataatacaa taccatttgt acatagtaaa caagatttat attccaatta
    12241 tacatcttag gaaaatgcaa actatttgtt atatatatat gcaaaatata atactaaatt
    12301 aaactattaa taaaacacta tatagcctta aaatcatcat tttttacccc tatatgagat
    12361 gatactatag ttataatact tagtactata aatactacaa aacctttttt atatccatca
    12421 actatattat atgaatttac ttgtaaagag atagctataa tcaaattaat aattccactt
    12481 cctataaatt caaaagtatt aataacacta gttgccagtc cagaataaat ttctttattt
    12541 ttatacttgg catcattaaa aacctgtaga tgtgacataa ctatacatcc cataataaag
    12601 aatgatattg gtaaaactat aattggtggt tttacctggt atacaactac aattacaatc
    12661 catataaaca aatctatcat tgccccaaat tttattatat taaatttgct agaccttatt
    12721 ttagcaaata caaaatccat aattattgaa ccaaatataa atccatatgt aaaaaatgat
    12781 actataaacg cagaaaaact cttactcaca ccataaacgt ccattatgta tcttactccc
    12841 cacaagctta taaatgcact tgtaagaccc acaaaagaga acattatcat tgaattatac
    12901 catgttgatt tgttctttat aacagactta ataccatcaa caatattaac cttctctgac
    12961 ttttcatagg gttcagtatc tatattgaat ccatactctt ttggtgtatc tctaacaata
    13021 atatacataa aacatccaat tacaacacca attataccga ttaataaaaa tgaatttctc
    13081 caaccaacaa gttcagacaa aaacacaagt ggaaaagtag caagaactcc acctaaattt
    13141 cctacaaatg aaaatttagc tgttgcagaa gcaaattctg acttattaaa ccatctccct
    13201 tgaattttta aaatacaaag taatattact gatgttcctg cccctacaat tacacgagaa
    13261 atatatgcta attctatact ttgaatcaaa ccaaataaaa ttgaccctaa acttgccatt
    13321 attattccaa gactgctaat ttttcttgct ccatatttat caatcaaaat tcctgcaggt
    13381 atttgcataa atgcatatgc atataggcaa aaggatgcta tatttgaaat ttgtatagaa
    13441 ttaaaaccta actcatttgc taaattgtct gaaacaacag cagttgacat ccttaaaaat
    13501 aatactaaaa caaatgaaaa tgtaattata ccccaaacac tccacttagc ctttgtataa
    13561 cttttcatat cttctccctc aaatccatat atttttaaat tttcttgata taacttatat
    13621 aaagtgtata gttattctta caaattttta cacgcaatat cattatacaa catatgtata
    13681 tatttaacta gaaataaaac actattcata ttatatacat gaattaacta cataaataat
    13741 aagagccatc ttttaacatt actcaaaaga tggctccata taaaaacatt tattctaaat
    13801 ttaatttttt atctaatata aattttgtag cgaaaaataa cgctattgca actacaagac
    13861 taccaagtat tgcaagcata aaactaggac tctgataaag atgatatacc atatttgtat
    13921 cttcatttgg taagatgctt ccaactgcat ctccaacaat tgatattact atatttatag
    13981 ctataaaaga agcaaatgcc actatgtttc tatgtttatt gaattttggt aattgtccca
    14041 ttgagattga tgtatataat aacaaaataa atgatgaata tgaaataaac attactacta
    14101 ctaataataa aatatctaaa aagttttcag aaatccattt aaaactagtg ttaaacaaat
    14161 ctaaaatttc aggtagtaaa attccagaag taccaaaaag cataagaact ccaaaagtga
    14221 atactgcaac tataaaacta agtacagcgt atataagtgc tgttatacat ttagataata
    14281 taagtgaact agtgctaaca ggaagagtaa acattaaata accttcatcc cccaaaagat
    14341 tttttctaaa cctttgaatt gtaactacaa tagttaaaac tcctaatgcc ataaaaagac
    14401 ttgttaatac tagtattcct attccctgta cctgaagtat atttgtattc ataaatattc
    14461 cgttaaatac agctactatc aatatggcta tatataacgg tataaatatt cttccacttg
    14521 cttttaactc atattttaat aatttaccta acatttaaat tcctccctaa ataatgcatc
    14581 tatagatttt cctttttctt ctcttattgc ttcaacgtct ccttgtaaca ctatttctcc
    14641 ttttgatatg aatattatct catcacatat gttttctatt tcacttatta aatgggtagc
    14701 aatcagtaaa gtactatctt cagaataatt ttttaatata gtcttaagta tatatgacct
    14761 tgcagctggg tcaacaccac ctataggttc atccaatata tatatgctcg catttcttga
    14821 cattactaaa ataagttgaa ctttttcttt agttccttta gacattgtct ttagcttttc
    14881 atttacatca atatctaagc ttttaatcat ctcatttgct cttctaacat caaaatcact
    14941 ataaaaatca tagaaaaact taagtaaatc tgataccttc atccaatcat ttaaataagt
    15001 tctttctggt aaatatgaaa ctattttttt agtatctatg gatggtttca ttccatttat
    15061 cataatttca ccattatcag gctgtaataa tccattcatt agcttaatca ttgtactttt
    15121 gccacttcca ttaggtccta acagcccgac tatttttccc tttggtatat ttaaatcaat
    15181 attttttaaa acattcttag ttccataact cttgtttaca cctttaaatt caactattcc
    15241 atcttgagaa acattgctta tatttttatc cattttataa ttctcctttc agattttctg
    15301 taataagttt tagcatttca tctctatcat atccaagctt ttctaaggtt acttttaatg
    15361 tatttatctc cacattagct atttcctctt tcatagcttt tattttttcc ttatcatctg
    15421 ttacaaatct tcctttagtt ctctgactat aaactaaacc ttgtctttca agttctgtta
    15481 gagccttttg cattgtattt ggattaactt ccgcatcttc tgctagtgct ctcactgttt
    15541 ctaatttaga tcctatttta atttcccctg aaattatttt taactttaaa tgttctacta
    15601 attgtatata tataggtttg ttattatcaa gttcccattc catgttcatg cctccttatt
    15661 atattattgt attaagttgt taatacaata atataataat acaaagtata tgtcaattac
    15721 tttttagtaa aagtataaaa aattttacaa aaaaaatgaa cactacatta gagtccattt
    15781 ttaacacaat ttattaaatt ctttatatta cagattaatt accctcttac caagttagat
    15841 acaattaaat gttcaaattc ttcaattggc attggctttc caaacaaata tccttgtgcc
    15901 atatcacact ttacttcttt taaaagttta aactgttctt ctgtctcaac tccttctgat
    15961 atgactttca tacccaactc ttttgccatt tctattactt tagtaacaat tattttgctt
    16021 cttttactat tataagtttc cataataaat ttttggtcaa gttttaagat atccatagga
    16081 atttctttta acatatttaa tgatgagtac cctgagccaa aatcatccat agatattaaa
    16141 aatcctattt tttttaaatt attcattata tcaattaaaa tatcaaaatt atcaaatatg
    16201 atactctccg ttaattcaag ttctatatac tttgcaggta tattgtattt atcaattata
    16261 tttttgtatg tttctataaa attattgcaa tataaatgta tccttgaaac attaactgaa
    16321 acagtaactg gatttattcc tgcatcaatc cattttctaa ggtatataca tacttcttca
    16381 agcacatata aatctaaatt tataacaaaa ccgtttcttt caaaaaatgg gataaattca
    16441 tttggaggta ttagcccttt atcaggacgt ttccacctaa caagtgcttc tgaaccttgt
    16501 atctctcctg tattaagttc tatcttaggt tgtaagtata taataaattc tttattttct
    16561 agacttgaaa acattgtatt ttccatctct ttttctttta tctcttggtc atgaatctta
    16621 ttatcataaa atgcatatga acttttatgt cctccctttg ccattttatg aggtatatta
    16681 gccctatcta tgattgagtt gatatctcta tcttctggaa gtataaaata gattccacaa
    16741 cttaaaacta acttatattt ttcttcttga ttattattaa acaagtctaa ttcttcaaat
    16801 atagttttta acctctttgt tatatcttct tcttctatat atttcataat tataataaaa
    16861 ttatccgcag atattcttgc aaacatttcc tcttcaaata tattcctctt aataatcttt
    16921 gttaaattac gcaaaatttc attcccaact tcatatccaa ataaatcatt tatatattta
    16981 aatttatcaa tatctatata taataaagca tactttactt gagtattttt acatagtatt
    17041 ttgttagcat caattacaaa tttatctata gtatttgctc ctgtaagatt atctgtatat
    17101 gctatccttt gcaatttaat tttaaaattt aatttatcat atattatata gaatataagt
    17161 attacacata ttatagccat aataagtatt atttgtattg catgtttgct aataagctga
    17221 ttattagaca caatagactc aaagttattt atggagttgt ttttatagag tttgcataaa
    17281 gaatagactg gtgttgtgta tgtaatttgt aaaattatac aaataaataa aaaaaacatc
    17341 aatttttttt gtaaattcct cataaaaatt tcctcccatt aaaacacctc tattttatta
    17401 gcaaataagt acatctcaaa agtttccatt tcttgtaaaa tggatagttt atatctactt
    17461 taattttgta aaattttctg ctactataat tatagaatgt agtatattca ttgtcttaac
    17521 tattgattta tatatttcat ataggtataa tagaaaataa attggattat tttggcatat
    17581 aattatttat taaaaggtga ttatttttgt ttaaattgtc tgaaaaattt tatttttata
    17641 ttataatttt aatattttct tttc
//
LOCUS       DAMJWU010000073        17477 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMJWU010000073 DAMJWU010000000
VERSION     DAMJWU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.074"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            290..1123
                     /locus_tag="PC460_002592"
     CDS             290..1123
                     /locus_tag="PC460_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2601472.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1216..2148
                     /locus_tag="PC460_002593"
     CDS             1216..2148
                     /locus_tag="PC460_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2601473.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2200..2682
                     /locus_tag="PC460_002594"
     CDS             2200..2682
                     /locus_tag="PC460_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2601474.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2694..3563
                     /locus_tag="PC460_002595"
     CDS             2694..3563
                     /locus_tag="PC460_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2601475.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3606..4361
                     /locus_tag="PC460_002596"
     CDS             3606..4361
                     /locus_tag="PC460_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2601476.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4403..5224
                     /locus_tag="PC460_002597"
     CDS             4403..5224
                     /locus_tag="PC460_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HDF2601477.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5229..5990
                     /locus_tag="PC460_002598"
     CDS             5229..5990
                     /locus_tag="PC460_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF2601478.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLA
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6151..6408
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6521..6760
                     /locus_tag="PC460_002599"
     CDS             6521..6760
                     /locus_tag="PC460_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2601479.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7064..8059
                     /gene="ilvC"
                     /locus_tag="PC460_002600"
     CDS             7064..8059
                     /gene="ilvC"
                     /locus_tag="PC460_002600"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF2601480.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8112..9800
                     /gene="ilvB"
                     /locus_tag="PC460_002601"
     CDS             8112..9800
                     /gene="ilvB"
                     /locus_tag="PC460_002601"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF2601481.1"
                     /translation="MSGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(10161..11291)
                     /locus_tag="PC460_002602"
     CDS             complement(10161..11291)
                     /locus_tag="PC460_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2601482.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINRKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKIKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(11531..12037)
                     /locus_tag="PC460_002603"
     CDS             complement(11531..12037)
                     /locus_tag="PC460_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF2601483.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(12300..13889)
                     /locus_tag="PC460_002604"
     CDS             complement(12300..13889)
                     /locus_tag="PC460_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2601484.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IVFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            complement(13913..14476)
                     /locus_tag="PC460_002605"
     CDS             complement(13913..14476)
                     /locus_tag="PC460_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2601485.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            14629..14850
                     /locus_tag="PC460_002606"
     CDS             14629..14850
                     /locus_tag="PC460_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601486.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            14853..15668
                     /locus_tag="PC460_002607"
     CDS             14853..15668
                     /locus_tag="PC460_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDF2601487.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQPKLPTTYKEALQHLIEQVEVNEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHS
                     ETIDITRSDGMTDVKMTTKWTQKGRLFLYDLLKVNNILPDIEKEYSYQTSMLC"
     gene            complement(15847..16026)
                     /locus_tag="PC460_002608"
     CDS             complement(15847..16026)
                     /locus_tag="PC460_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDF2601488.1"
                     /translation="MSTSLPKYTLRINRVLLEKIKYIAESEGRSANKEIEQIIKKHIE
                     DYEQRKGEIKINIEE"
     gene            16162..16341
                     /locus_tag="PC460_002609"
     CDS             16162..16341
                     /locus_tag="PC460_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601489.1"
                     /translation="MNSNKKRITVRMPEKLNEEITKKSKYLGLTKNSFILDILWKEFE
                     LLEYRSYKKEVDKHE"
     gene            16334..17125
                     /locus_tag="PC460_002610"
     CDS             16334..17125
                     /locus_tag="PC460_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDF2601490.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDSRNISNNFNNNKDKFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFASKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVETIKDVVSLDS
                     NSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            complement(17208..17402)
                     /locus_tag="PC460_002611"
     CDS             complement(17208..17402)
                     /locus_tag="PC460_002611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601491.1"
                     /translation="MKFVYVKIFNNWIDITDIGSINGCNAIEYINENLDKMYSSNYVS
                     INYKDKNYCIHPSCIQIVTK"
BASE COUNT         6985 a         1899 c         2830 g         5763 t
ORIGIN      
        1 tttgtttaat atttctaatt tataaaaaaa aaattataaa ttttaaaaat tataaatgac
       61 attttaacta gaattaattg taaaaaacac tatataatac tcaattttca acattctata
      121 caattataca gaaattatac agcataataa tcaccgaaat aacaaatttt aaaaattaag
      181 caaaactttt tgtcgaagtt caacgtctat atatatgtag tgtaaatttt tgggataaaa
      241 tactacaaaa atataaatgt aaaaatatag taatattgga ggtcgaacga tgagaaataa
      301 aaagaaaaaa ataaatagaa agaagttgta tttgctttta agtgctattg tagtgtgtct
      361 ggcttttata gtagttggtg ttcgcgtcag ctctttaaat atgaaggaaa atgaaattag
      421 tagaaatgca aaactgtcaa gttctactat tacagatata gtatctaata catcaataga
      481 agttagtaaa gggccaagta aaagttctgg aaaaatagca tacataacta tagatgatgg
      541 tccatctaaa tttactgacg agatgataaa aactttaaat aaatacaatg ttaaagcaac
      601 tttttttatg atagatggga atatgaagga atatccacag caagttaaaa atataattaa
      661 aaatgggaac acagcaggtt ttcatagtgt atcacatgat attcacaagc tttatgttac
      721 tagtacatca gcaaaagagg aatttgatac aaatgatcaa actttttata agataactgg
      781 taaacattca aaagtaataa gaatacctta tggaagcaaa ccatatacac cacaagcctc
      841 ttatcaggct ttagttgatg ctggatataa aatttgggac tgggacttag atacagaaga
      901 ctggagatct aattcttctc aaattgtaca aaatgtaaaa aatcatatta agaatagaaa
      961 aggtgaagat aaagaccaac ttgtagtttt aatgcatgag aaaaaacaga gtgcagaagc
     1021 tttagattca gttttaaaat tcttatcaga tgaaggttat gaatttgcgc cagtagatca
     1081 aaaccaaata ccaaaaaatt attggttacg taatttagag tagttaaaat ataagaatat
     1141 tgattgatat ttttagaaat tgagaataaa aatgagtgag gtgtaggtat attgaataaa
     1201 aaactttata taggaatggt cggaatattg tctttgatga tggttggttg taataaaagt
     1261 ttagcaaaag taaatgatgt ggaaatcaca aaagaacagt acaaaaaaac aaaagcagtt
     1321 ttatctgcta ctaataatta tataaatggg cagtctttag atgaattaga aaaaactttg
     1381 gataaaaaag gtagaaataa attagaaaat gttataatat cgtttatggt ggataatgaa
     1441 cttttatatc aagaggctaa ggataaagga ctaactccaa gtaaaagtga agtagactca
     1501 aaatatcaag aactagaaga taagatgaat ttgaatacaa gctataagga aaaaatggac
     1561 aaagcaggtg ttgataaaga ttatttaaaa caagaaatat ctagagattt agcaatagat
     1621 aaaaataaaa aggcctttga agatagaata aacataagtg ataatgatat ggaagcctac
     1681 tatacaagtc ataaaaaaga ttttaatgta gaagaagtaa gtgcttctca gatacttata
     1741 tcgactttag ataaaaataa aaaagaagta agcaaagata aaaaagaagc tttgaagaag
     1801 aaggcagata atatattgac taaaatcaaa aatggagaaa gttttgaaag tttggctaaa
     1861 aaatattcgg atgataaagc aactggaaaa aatggaggac agctaggata ttttacaaag
     1921 gatgataaaa atgctgagtt tacaaaggaa gtgttcaaat taaagaaaaa tgaagtttca
     1981 aatgtgtttg aaactagtta tggatatcat atagtcaaag ttactgataa aagagaaaga
     2041 caaaaaagtt ttaatgagtg tcagagttta ataagagaat ctattttaaa tgaaaagtat
     2101 attgagcata taaaaaaact gaatgaagat gctaaaatag acagatagaa tttctaacag
     2161 ctcaatccta gcctgtaatc ggagggagat tttacaggaa tgtttaagag tttaaagaga
     2221 acgaaagttg agaaatacat tatcgataat aaagactctt tttatagaat agcatatagt
     2281 tatacgaaga atgaagaaga tgccttagat gtggttcaag aagccatgta taaggcacta
     2341 tactcagtgg aaaatattaa agaagtaaat tatataaaga catggtttta taaaatttta
     2401 gttagaacga gtatagattt tattagaaaa aaccgtaaat ataataatat gacagatatt
     2461 gaccttatag atgagactgg agagtatgat aaatatactg atttggattt aagaagagct
     2521 ttggaagaat tacctattga atacaaatct attataatat taagattttt tgaagattta
     2581 aagattgaag aagtagcaat aatattggat gaaaatgtga atactgttaa aactaggtta
     2641 tatactgctt taaaaaaact aaaattaaaa attgaagagt agggataaaa agaatgacaa
     2701 gcaaagaaag gttaaataag ttaaaagatg agtatcataa aattccaata cccaagaaat
     2761 tagatactat aataaatcat gaaaaaatag aaaacatata tcgagagaaa aataaaaaag
     2821 taaatagatt aagatttaaa gttgcaatag cctttgcatg tatatttaca gtattagtaa
     2881 atataagtcc tgtatttgca gacaattttt ctaagatacc tgtaattgga gcaatagtag
     2941 aagtaataac tataaaaaat tatagtttaa aaagtgaaaa ttatgaagct gagattgata
     3001 ttcctaaaat acgaggttta aaagataaaa acttagaaca aagattaaac agtagtttta
     3061 tggaagatgg aaaaaggctt tatcatcaat tccaagagag aatggagaag atacaatcat
     3121 caaagaataa aggatataaa tctttaagtt tgagttatag tgtaaaaaat aattctaaaa
     3181 aatttctatc aatagagatg acaaaaaatg agatagaggc ttcatcatat gtgagtaagg
     3241 tacattatac tattgataaa aaaagacaaa tagttttgac tttgccaatg ctttttaaag
     3301 atgataagta tataaaagtt ataagtgata atataaaaga acaaatgaga gaacaaatga
     3361 aaaaggatag tacaaaatcc tattttatag atcaaaaaaa agacttgcct gttgaagatt
     3421 ttaaaacaat taataagtat caggattttt attttaataa aaatgaagac cttgtaattt
     3481 gttttgatga atatgaagta gcacctggtt atatgggcgc agttgagttt gtaataccat
     3541 ataaagtaat aaaagatttg taaattgaat agtagtttat atataaatct tttaaggagg
     3601 acaatatgac tacatataaa aaacttaaaa aatctatatt taaaattgct atattttcaa
     3661 tatgcttatt agtttcatta gtagatacaa accctatatt tgcaggtagt tcctttaatg
     3721 aaacttcaaa tagtgatacg ataaaggtgt taaatgctaa gagtatcaac tttaaacaag
     3781 acaattatga agtgaatgtg agtattccta aaataaaagg tttaaaagat aaaactttag
     3841 agaataaatt aaacaatgag tttttgcaag atgggaaaaa aatttacagt gaatttcaaa
     3901 ataaaatgaa aaaggaaaat aataagggtc atagatacct aaatgtaatc cataatgtca
     3961 aaaataattc taaggacttt ttatcaatag aaatgacaaa agaagagata gaagcttcct
     4021 catctataac taagaagcat tacactgttg ataaaaatag aggaatacta ttaaatttac
     4081 caatattttt taaagatagt agatatatta gagctataag tgataatata aaagagcaga
     4141 tgagaaatca aattaagaaa gatgatacaa aatcttattt tatagaccaa gataaagact
     4201 cgccagtgaa agattttgaa tctataaata agtatcaaga tttttatttt aataaaaatg
     4261 gaaatcttgt gattagtttt gatgaatatg aagtagctcc tggttatatg ggagcagtag
     4321 aatttgtgat accaaataaa gtaataaaaa gactaagata gatatataga agatttttag
     4381 atagactggt aaggagaatt atatgaatat atatgaacaa gcaataagat taatagaaaa
     4441 taatgaggat ttcgcatttg ctacaataac gtctcattca ggttcaacac cacgtgaaac
     4501 tggagcaatg atgattgtga aaaatgattc tactatattt ggctcagtag gaggaggtag
     4561 tgtagaagca gaatgtataa aacatgcaat taatgttata aaaaacagag aatcaatgtt
     4621 gtataaattt actctaaaca aatctgatgt ggcaaaacta ggaatgatat gtggaggtac
     4681 tggagaaata caaatagatt ttatagatag taaattaaaa agtaatatag aaaaatttaa
     4741 taagagacta aaggaaaata caagtaaagc atatatattt ggtgcaggtc atatttcaag
     4801 agatgttgct gtgatactat ctcttttaga atttagaaca gtagtaatag atgatagaga
     4861 agaatttgct aatcatgaaa ggtttccaga atcagaagta gtagttttag attcttttga
     4921 aaacatacca gattttccca ctgacgaaaa tagttatata attattttaa caagaggtca
     4981 tctatatgat tcaagtgctt tagaatgggc tcttaaaaga gaggctggtt atataggtat
     5041 gattggaagt agaacaaaaa ttggattgac ttatgaaaaa ctcatgaaaa aaggttttaa
     5101 gaaggaagaa ttatcaaaag tacatgcacc aataggcata aaattgaatg ctcaaacacc
     5161 agctgaaata gctgtatgta tagctgctga acttataaat tgtagagcta ataaggaaaa
     5221 ataaactaat gaacacaggg agtaaagata ttgatacttt aaaggttaat tttagaggga
     5281 ttgaaataaa agcagtttta gtatataaaa atagaaaaaa tatttctata aaaatagatc
     5341 catttggcaa aataactgta atgagtcctc ctaaaatatc caaaaaaata ataaaagata
     5401 taattattga aaaaggagac tggatactta aaaagtcaga aaaatgtaaa ggaagagagg
     5461 aagtatataa acaaagaatg tttataactg gcgaaaaatt tctttattta ggtgaagagt
     5521 accatctagt tataaaagaa ctgttgagag attctgtcaa aaaaagtaac tgtagaataa
     5581 ctattaacga atatcaaata gttgttcaga caaatgatac aagtacagat tttattaaaa
     5641 aatctttaaa atcttggtat aagatggaaa gtgaaagaat agtattagag agaatagatt
     5701 ttcttaaatt aaattgtgaa ataatgaagc aattagttcc agcgagtgtt aaggtaaaag
     5761 aacaaaataa gagatgggga agttgtacag ctcaaaaaaa tatttatata aactcaaaaa
     5821 tatctatggc aagattagat gtaattgatt atgtaataat acatgagttt agccatttag
     5881 cacatatgaa tcactctaaa aaattctatg atttagtaaa atccattatg cccgattata
     5941 aagataaaga aaattggcta aaaataaatg gatataaaat aataatctga aaacgattat
     6001 tccaaaatat aggcgtaaag tataaaagaa gtattattgt gaaaaaaata aataatctga
     6061 aaattttaaa aaaagtcatt gacattgtaa atgatagaga atataatcta tattaacaac
     6121 aaaacagaat tgaaatataa aattaaaaaa ttaaaagcaa tgacagagac aacaatacta
     6181 atagtgttat ccacagagaa ccaatgttgc tgagaattgg tgttaacagt agtataatga
     6241 tcactctgga gcttttgtac tgaatatttt tagtaagtac aaacgtatgt caacgttaca
     6301 tgactaaggt taaggtaatt aattacccga cctgaaaaaa gaggggtaga tgtatttttt
     6361 atatctacaa ataaggtggt accgcggaat ataactttcg tccttataag taatatttat
     6421 attgcttata aagatgaaag tttttttatt ttaagtgata taaataaatt taatccatat
     6481 aaaattaata aaaaaataat aaaaataagg agggattttt atggaaaata ctgtatatgc
     6541 taaattaaac caaggaatag attctttttt aagagtggct atgactctaa gaagaagaga
     6601 ggttgatata caatcaataa gtatgactgt agataatatg aataattcag gtataaaatt
     6661 aatagtaaat gaagaaaaga catccttaga ttctgtctta aaccatatga aaaaactaca
     6721 tgatataaga gagataactg ttgaaagaat gagacattaa ttaaatatat tttataaaac
     6781 taaagataaa tatgcttatt ttaaaataaa aaactaaaga taaatattaa ataagaaaaa
     6841 tcttagatta aaatataagt gtcattgaat ttattagttt atcacagtta ataaatttat
     6901 tagtttgttg tagctaataa aaactgaaaa ttctaaaatt ataattgtaa gattatataa
     6961 atttagaatt taaatctgtt gaataatatt aaaacaatga taatgagtat gaatttctaa
     7021 taaaaataaa tatcataaaa ataagaaaaa gtattgggag gaaatgaaaa tggcaagaat
     7081 gtattatgaa aaagatgtgg atttagaggt tttaaaaaat aaaaaggttg cagttttagg
     7141 ttatggaagt caaggacatg cacatgcaca aaatcttaga gataacggtg tacatgtagt
     7201 gataggtctt tatgacggaa gtaaatcagc acaaaaagca aaagaagatg gatttgaagt
     7261 taagagtgta gcagaagcta caaaagaaag tgatttgaca atgatgttaa tgcctgatga
     7321 aaagcaaaag aaagtttatg aagagagtgt aaaggataac ttaaaagaag gtcagacatt
     7381 agcttttgct catggattca atatacatta taatcaagta caaccaccag aatttgttga
     7441 tgttgtaatg gtagctccta aaggacctgg tcacttagtt agaaatgtat ttacaaaggg
     7501 aagtggagtt cctgcactat ttgcagtata tcaagactac actaaaaaag ctacagagac
     7561 agttttagca tatgcaaaag ggataggagc aacaagagca ggtgttttag aaactacttt
     7621 taaagaagaa acagaaacag atttatttgg agaacaatca gtactttgtg gtggaataag
     7681 tgaacttata aaattgggat ataaaacgct tgtagatgca ggttatcaaa aagaagtagc
     7741 atattttgaa tgtttacatg aaatgaaact aatagtcgac cttatatatg aaggtggatt
     7801 tgaaagaatg agatatagca taagtgacac agcagaatat ggagattatg tatctggtaa
     7861 aagggtaata actgatgctg caaaacaagg tatgcaaaat gttttagagg atatacaaaa
     7921 tggaaaattt gcaaaagcgt ggattaaaga aaatgaagaa ggacgtgaaa acttcctaaa
     7981 aactagagaa gaagaatata atacagaaat tgccgaagtt ggtagaaatc taagaagtat
     8041 gatgtcattc ttaaaataaa tatatattaa atggattagt ttgagggaaa gagtggtgta
     8101 tagtagtgcg tatgagtgga gcaaaggtaa ttttagaatg tttaaagaaa gagggaattg
     8161 acacaatatt tggatatcct ggtggagccg tgatacctct ttatgatgct ctatacgatt
     8221 attcagatga ttttaaacat ataaggacat cccatgaaca aggtttagtt catgctgcag
     8281 atgggtacgc aagaagtact aatacagtag gagtttgttt tacaacatct ggtccaggag
     8341 caacaaatgc cataactgga atagcaacag cttttatgga ttcttcacct atggttgtaa
     8401 tttcagggca ggtgccaact agtttattag gaaaagattc ttttcaagaa attgacataa
     8461 ctggagcaac attatctatg acaaaacata attatttagt tagaaacact aaagaattag
     8521 ttcctacaat aaaagaagct tttagagttg caaattcagg tagaaaagga cctgtacttg
     8581 tagatgttcc aaaagattta tttttagcag aaatggattt tagtggtgag gactatgatt
     8641 tatgtcaaat agatgattat atggattata agagtgattt tgatctagat gatgaaacta
     8701 atattaagct tttaaatgaa gcaatagata tcataaaaga atctaaaaag cctataatat
     8761 atgcaggtgg aggtgttaaa tcttcagaca gtgaggaaat tcttgaaaaa tttgcaacta
     8821 agatagatac acccgtacta aatacactta tggggcttgg aaatatagat agaaagaatg
     8881 aattatctct tggaatggtt ggaatgcatg gaagtagaga aagtaattta gcactttcta
     8941 attcagattt agtaatagcg ataggtgcga gatttagtga tagagtgata agtaaaagtt
     9001 ctgaatttgc taaaaatgca aaaataatac acatagacat agacccatct gaaataagta
     9061 aaaatataga atctaatgta tctttggttg gagatgtaaa gctagtcctt agtttattaa
     9121 ttgaaagggt tgaaagtaag aataatagca attggaaaga agagataaag agatttagaa
     9181 agagtgaggg tgtacaaaca tatgaatttc atccacaaaa tatactaaaa aaaataaatg
     9241 aaaaatatga aacattaaag aaacccactg tggtagtaac tgatgtcggt caacatcaaa
     9301 tgtgggctgc taagtactgg aattttaaag gtaataaaag ttttataaca tcagcagggt
     9361 taggaactat gggatttggt ttaggtgcag ctattggaac taaagttggt aatgtagata
     9421 aaaatgtagt tttagtcact ggtgatggaa gttttagaat gaactgtaat gaactagcta
     9481 cagttgcaaa ttataatgta cctatgctta ttttattact caataataga acattaggaa
     9541 tggtaaggca atggcaaaag ttattttcca atcaaagata ctctcaaact gacattaatg
     9601 aaaatgtgga ttatgttaaa ctcgtaaatg catataatat tgatggatat aaggtatcca
     9661 gtatggaaga attaggaaag gcgctagata tgatagattt taataaacct gtatttttgc
     9721 aatgtgatat agataaggat tatgatgttt atcctattgt agcacctaat gatgcattgg
     9781 aaaatttgat atgtaactaa aacttatact gaatattgat aaaatttttt attaaatttt
     9841 tattggtatt cagtttttgt tgtttagaga tagattggca ttttttattt ttgcaaataa
     9901 atcaatgact atataattaa gaacttatta aattttaatt atacaattta gaatagtatt
     9961 acaacttaag taaatattaa gttttatttg ttagatttat tattaatacc ttatattagt
    10021 tatggttgtt atatactcta aagccttgaa attacaagta tatagatgat tatataaatt
    10081 tgataatttt agcttttaaa atagataaat tagattttgc cacccatttg ccaccgtatt
    10141 atatttttgg gtggcaaact ctaatttgta attttttcaa atatatctac agtttcattt
    10201 ttcattttat cagttacatg tgaataggta tccattgtag ttgatagttg gctatggccc
    10261 aaacggtttt gtatgtcttt aatgttagca ccattttcta ataatagagt agcatgtgca
    10321 tgtcttaaag aatgaaaatg gaagtcattg tttaaagcta ctcgaatttg tcttactata
    10381 gtgtctaaag tgtgagtatt cacttgttga ccattttctt tggtacatac ccagtcacta
    10441 tcaaagtaaa attctccata ttttaatttc attttctttt gatataattt atgttctttt
    10501 aatgccttta ttaaagtgtc acctgtaaat atagttctgc aagagctttc tgtttttggt
    10561 tgccctaatt caaacattcc atttggtttt ttaatcaaag tatgtcttac tgtgatagtt
    10621 ttattatcaa ggtctatatt atcccatttt agtgcaataa tttcacctct tcgcatacca
    10681 gtatgaaatc caattagtaa aactatacgt tgaaatgaat cttgaggaaa tatatttagt
    10741 atttgattaa attcttctaa tgtaatagtt ttaattttat tagtttctgt tttagattta
    10801 gtttttggta tgctcacata ttgcataggg ttttctcgta tatgtttgta aggatggact
    10861 gctgatttta atgacctatg taatatggct tttaatactt gtaatgtatt ttgagagtaa
    10921 tcctctttgt actttctgtt tatgaagttt tgtagtattg caggagttaa agcttttact
    10981 ttgtaagctc ctagttttgg ctttatatgt ttttctatgt ttattcggta gctttcttga
    11041 gtgttgtatt tacagttaag taagacatat tctttgtacc aaaagtctaa gtagtctgat
    11101 aaactgatat tgctttcttc aaatactatg ccagagtttt catattcatt tagtgcttct
    11161 cttaaggctt tttctgcttc ttttttagta ttgcctccaa ctctttctac tttttttctt
    11221 tttccttcta ctatgcctag gtcaaagtag taataccatt tgttacttct ttttcttact
    11281 ccacctttca taatagtatc cctccctttt agaatgtatg tttgtttggt gtttatataa
    11341 aagagcagat taactgctct ttatatactt ttgttatgta caatattatc ttaattttgt
    11401 ttatttggtt tttacttaat tattttctta atattcttac aattttttag cttttatgtg
    11461 tagtttatta taataatatt gaaatatata tttattaagt aaaaaatcta taaaagtaga
    11521 ataaatgagt ttaaaaaata tcctcatata tgtagaaatt tggcaaatta gttaatacta
    11581 aaaatctatt atttcctaag tccaacatgc ttttcttttt tgatagaaat tctaatcttt
    11641 ttagtaagaa gttgatgcta acttgtaatt cttcagctat ttcgtataca cttgtagcgt
    11701 gtgaattaat aacatgtatt atttcttctt ctgttataag aaattcacat gcccatttta
    11761 gtgctttgtt ttcagttttg tctatcaaga ttttattttt gtaactgttt tttgaagata
    11821 catagtttcc aacactggta aaatgatgtc ctaattcttc tgctaagatt tctattagtt
    11881 tagcattgtt ttgttttaat gaattaagta atgatataat ttttagtcct tgtctgttta
    11941 tatacaatcc ttttatgtca tctgctattt tgtcagtgta gtaaatttct atctcttcat
    12001 tatttgctaa gtctaaaagt gcgtctagtt tgttcattga aatcccccta taaaaagaat
    12061 gtatttttga tttttgttat atataaagag caggtgtgaa ctgctctaaa tattcatttt
    12121 ttatccaaaa aagattggat aattttagct gtagacaaaa tatggattat agtatatgat
    12181 tataatataa ttacatataa tatatgtaag cacttacaaa tgtgtgttag tatagttagc
    12241 actaaatttg tatttgtgct taaagcgatt aaaggagttt tggagcggtg ggtagagttt
    12301 tactcactat aaccaagact cttttgcata ttagagtatt tcatgtagtc atatattcta
    12361 ataagccttc ttaaatttgt tttttcgctt gtttttgcag actctaaaat attaggattt
    12421 tcctcaaata ttttcttaag tagattttct aactgttcag tgtcaatttc tttttcttct
    12481 aaaaaagcaa taagtcttgt agctttttca aatgattgat gttcatcatt ccaaatacca
    12541 taaacacttc tattttttat gtaagtatta tttctatttt ttttaatact attgctaata
    12601 atattttcaa agtcactttc tagtgcaatt gtcctataat ctggaaattc aagtgtgcta
    12661 ttaaaaagat gtttatttaa tggtaattta ttatattgct taattaaaga tggataagca
    12721 tattcaagta attcatctga tgaaaattct aagtcattca tgactatatc acgataccat
    12781 tcatcaaaac ttaatccttt taatccttta agaccaaaat cacttgcctt gccaactgct
    12841 aaaactaaat cttcatcttt tattcttgta tcatcaacac taccaacccg aattttagca
    12901 gtaggtttgt tagtaattgt gaaatcgtaa aattcttgtt tgaacattct taatattcta
    12961 actggtaatt ttgatttttt ttcagataat gcctcataga gtaaattaaa atcctccagt
    13021 ttaattttag tcatagtaat cattttatta tcaaaaacaa ttgtatgact tgatatttct
    13081 atatcattaa agtttttttc atactcaatg aatatgaaac gttcctcgag ttttttagca
    13141 ttttcattag atagacaatt aactatagca ttaagtattt tttttatatt tacatctgta
    13201 acagagtaac caataaatat gataggaaac tcaacaaata tagtcattag cttagctgcc
    13261 aagtaggcac ttttgctgtc aaagtctata taatcagctt catttattac aatactattt
    13321 ggagaagaaa cacatccatg aattttataa atttctgcaa taccttgtat agatgaaaaa
    13381 actaaatttt cttgacctat atatgttgta taattatcaa cgatagtttc caaaaaacaa
    13441 tcataatttg ttgtaataaa tcctgataaa ctttttttag agatattact aagtttttca
    13501 acttcatctt gatatttatc tactatttta gaattatttt taatatacat agcaatttct
    13561 gccttaaatg gagaaactcc tgatttaaca atatctaaat acttagagtc taagcttcgt
    13621 atagaagaat ctgaaaacca ttttttatta aaatcatttt caataagttc tgcaattcta
    13681 ggatttatac ccatttttgg gttctctgat tttgctttat ttttataact tgtatatata
    13741 aagtcatcat tactaatttt ttctgcaaag acttttagta aatcctccca atttggaaga
    13801 ttatagtatc ttctagttaa accagaacca acaaataaaa atggtgtagc attaaatcgg
    13861 tttatgacat ctgatattat agatttcata taatttatca cctactttta atttattttt
    13921 tatacttatt tattaaaaat tcaatataat cattgagttg ttcttgagct tcctctggta
    13981 aatctttgtg aggattaact ctatgtgcag ctacagtgtc tatatcattt tttacaatag
    14041 acctacctaa taaataatca gtagtcacat taaatatttc agcaaggcta gatagcatat
    14101 aagcatcagg aactgtcttt ccttgttcat aataaccata agcgcttttg gttaagttta
    14161 atttatttgc aacatcttct tgagtcatat ttttttcagc tctaagttct tttagtcttt
    14221 tttgaaataa tgtattatta caagaagttt tgctagaaat attgcttgct ttattatcat
    14281 acctacctat taaataatca acagtagtgc caaaatattc agctaaacta gataacattt
    14341 ctggggtagg tgttctttta ccttgttcat aaaatccata tgcactagta gttatgttaa
    14401 gatagttggc aatttctttt tgcattacac ctttttcttt cctaagaaat ttcaatctat
    14461 ctgataaaat gctcatataa tcactcctct taataaagtt gcctttatta aataatacaa
    14521 caaatagttg taaaatgaaa taaaaaaata aaaaaactat ttacaaacaa catatagttg
    14581 tgtattatat aagtataaac aacaaaatgt tggtaaggag gatatagctt gaacaatcta
    14641 aaggaactta ggacagaaag gaatttaaag caaaaagatg ttgcattaaa agtaggtata
    14701 actactagtt attatggaat gattgaaagt ggaacaagag taccaagttt agcaatagca
    14761 ataaaattat ctaaatgttt tggagtatca atagaaaaaa tttttttaaa tacaaataca
    14821 acaagatgtt gtttaaaaga gaggagttaa atatgaaaga tttaaaaata gtaaaagtta
    14881 ataataaact aacaactgac agtagagata tagctctaat ggttgaaaaa gaacacaaga
    14941 ttttactaag ggatataaga aattatataa accaaatgga agaagccaat aaaaacatga
    15001 gtacagattt gtacccatct gattatttta ttgaaaatac ttatttagat gattataaaa
    15061 gagagaagcc atgctatgca ataacaaaga taggttgtga cttcatagca aataaaatga
    15121 caggcataaa aggtacggca tttacaggaa tatatacaaa aaaatttgaa gaaatggagc
    15181 aagtattaaa aaatgaacaa cctaaactac caactacata taaagaagca ttacaacatc
    15241 ttatagagca agtagaagta aatgagaaat tacaactaga aggaaaaatg aaagaccaag
    15301 taataaaaga actaaaacca aaggcagatt atacagatat gatacttaaa aacaaaggac
    15361 ttgtcacaat aactcaaata gcaaaagact atggaatgag tggaaaagaa atgaataaaa
    15421 tacttcatga aagagggatt caatataaac aaagtggaca atggctttta tataaacaac
    15481 accaagggaa gggatacact cattcagaaa caatagacat aactagaagt gatggaatga
    15541 ctgatgtaaa aatgacaact aagtggactc aaaagggaag attgttttta tatgacttat
    15601 taaaagtaaa taacatatta ccagatatag aaaaagagta tagttatcaa acttcaatgt
    15661 tatgttagta ctttgaaaaa taaatacaga atattcaaaa agaggtgatt taaaggttga
    15721 gtaatagaaa gaagtatacc ttgagtatta cgaaagaatt atacaataaa tgtaaagaaa
    15781 aagctaatca aagaggtatg tcagtgaatg attacatact atcagtgatt agcaagagta
    15841 tataaattat tcttcaatat taattttaat ttctcctttt ctttgctcat aatcttcaat
    15901 atgttttttt attatttgtt caatttcctt attagcagaa cgaccttcgc tttcagctat
    15961 atatttaatt ttttcaagca gaactctgtt tattcttaaa gtgtatttag gtagtgaagt
    16021 tgacataata atacctcctt tttaaagtct aaatgatgtc ataattatat cataaaaata
    16081 atttatagaa aatctataaa atatattgac ggcataaaga cggcgtgata ttattaaaac
    16141 aagaaagggg ttgacggcaa aatgaactca aataaaaaga gaataactgt taggatgcca
    16201 gaaaaattaa atgaagaaat aacgaaaaaa tcaaaatact tagggttaac taagaactca
    16261 tttatattag atatactatg gaaggaattt gagttattag aatatagaag ttataaaaag
    16321 gaggtagata aacatgaata acttacaagt aatagaaaga aataatgaga gagttctaac
    16381 tacacaacaa ttagcagatg tatatgaaac tgactcaaga aatataagta acaatttcaa
    16441 caataacaaa gataagttta ttgaaggtaa acattatttt ttattgcaag gtgatgattt
    16501 aaagaatttt aaaggcattc atacagaata tgaaaaccta aaatttgctt caaaaatgta
    16561 tctttggact gaaagaggag caaatagaca ctgcaaaata ttagatacag ataaggcttg
    16621 ggaacagttt gataatttag aggaaacata tttcaaagtt aagcaacata agccaacttg
    16681 tatagaagat gtattaatag aaagtttaaa agaaatgaaa gatttaagac ttcaagttaa
    16741 tcaagcaaat agcattgctt tagaagcaaa gacagaggtt gaaacaataa aagatgtagt
    16801 ttcattagac tcaaatagtt ggagaacaaa tacacatcaa ctaattgcaa gaatagcaaa
    16861 aaaacaaggt ggttttgaac atataaatat gcttagaaca gaaagttatg aattattaaa
    16921 taagagattt ggagttgacc tacatagaag attgatcaat aaaagaagaa aaatggcaga
    16981 agaaggtgta tctgaatcta aaagagagaa agttaacaat ttagatgtaa tacaagatga
    17041 taagaaactc atagaagggt atgttgctat tgtaaaagat atggctttaa aatatggaat
    17101 atcaagtgat ttaagcaaaa attaagttaa atcacaacag cactttgaaa actaaatata
    17161 aaataaaaat atctccctta aaagtttttt aaagagagat aaacacttta ttttgtaact
    17221 atttgaatac atgatggatg aatgcaatag tttttatctt tgtaatttat agaaacataa
    17281 ttactagagt acattttatc aaggttttca ttaatatatt caattgcatt acagccattt
    17341 atagagccaa tatcagttat atcaatccag ttattaaata tcttaacata aacaaacttc
    17401 atgataacac caactttcat tagaatttaa gttaattata acatggtgta ctgagataat
    17461 gtgttaagaa atagata
//
LOCUS       DAMJWU010000074        17406 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMJWU010000074 DAMJWU010000000
VERSION     DAMJWU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.075"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            139..885
                     /locus_tag="PC460_002612"
     CDS             139..885
                     /locus_tag="PC460_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF2601492.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            946..1620
                     /locus_tag="PC460_002613"
     CDS             946..1620
                     /locus_tag="PC460_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2601493.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            1989..3335
                     /locus_tag="PC460_002614"
     CDS             1989..3335
                     /locus_tag="PC460_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2601494.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3362..4762
                     /locus_tag="PC460_002615"
     CDS             3362..4762
                     /locus_tag="PC460_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601495.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYKNLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4972..6408
                     /locus_tag="PC460_002616"
     CDS             4972..6408
                     /locus_tag="PC460_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2601496.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            6752..7696
                     /locus_tag="PC460_002617"
     CDS             6752..7696
                     /locus_tag="PC460_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF2601497.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7865..8797
                     /locus_tag="PC460_002618"
     CDS             7865..8797
                     /locus_tag="PC460_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF2601498.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENTLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8902..9369
                     /locus_tag="PC460_002619"
     CDS             8902..9369
                     /locus_tag="PC460_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF2601499.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9613..10467)
                     /locus_tag="PC460_002620"
     CDS             complement(9613..10467)
                     /locus_tag="PC460_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF2601500.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10633..12597
                     /locus_tag="PC460_002621"
     CDS             10633..12597
                     /locus_tag="PC460_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2601501.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12597..13058
                     /locus_tag="PC460_002622"
     CDS             12597..13058
                     /locus_tag="PC460_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2601502.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            13061..13381
                     /locus_tag="PC460_002623"
     CDS             13061..13381
                     /locus_tag="PC460_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2601503.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13397..14497
                     /locus_tag="PC460_002624"
     CDS             13397..14497
                     /locus_tag="PC460_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2601504.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14617..17217
                     /locus_tag="PC460_002625"
     CDS             14617..17217
                     /locus_tag="PC460_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF2601505.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDENIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYHIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6927 a         1782 c         3001 g         5696 t
ORIGIN      
        1 atatattttt atattttgta atttcaaatt aataaataat tagatttaat aaacaaaaaa
       61 taaaaatgtt gattatatta ataaacttta atataatata tttatgaaaa atgtaaactg
      121 tgaaatggag gtagtaacat gaaaattgaa aatctttcta ttgatgatat taaacgaggc
      181 tatatattta atgatgattt aaattgttat agttgcatta aatgtacaaa aacatatgag
      241 gtaggagaaa tatataaaat aggtaataga ttctttgaag cttcaaaagc tattgaactt
      301 catatggaat tagaacatag tgattattta gagcaattgc ttaatagtga ttcaaaatac
      361 aacacattga ctgaaaatca aaaacaatta tttcaattat ttattagtga tttaccagat
      421 aaggagatag caaaacaggt tggagtatcc acatctacta tacgccatca aaaatttatt
      481 ttcagagaaa aagcaaaaca ggctaagctc tatttagcac tatatgaatc tgtatttgaa
      541 gataaatcta ataaaagtag tactataata ccaatccatg aacatgctcg aatggtagat
      601 gaaagatatg tagtaactga agaagagaga aaacacattt tggatacatc atttgaaagt
      661 atgaatcctt taaaattaaa agtattttct tcaaaagaaa agaaaaaggt cgtaatttta
      721 tctaagattt tggaacaatt tgaacgtggg aagaaatatt cagaaaagga agttaatcag
      781 attttagaat ctatatatga tgattttgca acaattagaa gatatctaat tgagtatggt
      841 tttatgagta gaaataaaga atgtacaaaa tattggttaa cataattaaa ataataaatt
      901 ttactaatgt gtaatgttat ttttatctag ggggaggagt caaatatgaa tgatgaatta
      961 aaatctattt tatacaataa aggtgtagac attgttcgct ttgttgatat atctgaattt
     1021 ccaataaatc aaacacaagg tttttctaaa gcaattttat tttgtatagg gttatcaaaa
     1081 aaatttatta cagatatata caataactta cctacagata gtgatgagtt tttggaaaaa
     1141 gaggagaaag tagaagaatt agctgactgg atttcaaaat atattcaaaa taaaggatat
     1201 agagcatatt ctcaatcgga gaaaaacaat ctagaacatg gatattttga aaaagcctat
     1261 attaatccag aaatgcaatc agggattagt cctttaccac ataagacaat agcaaatatt
     1321 agtggcattg gatttatggg aaaaaataat ttgtttgtaa cagaggaata tggatgtgct
     1381 tttagcatgt gtacagtact tacagatgct ccaatttctg ttgaaagata tcctttgatt
     1441 gattcaaaat gtatggattg caatgtttgt gtagagaact gtcctgcaaa agctatacat
     1501 ggaaatgaat ggactttacc tggaaaaaga gaatctatta ttgatgtttc aaagtgtttt
     1561 tgtgtattga ggtgtatgat gagttgccca tggtcattaa gatatgcaaa tcaaaaataa
     1621 ctttactaga gaaggtatta aattgaatat tataaaattt ttaaaatgta agattatata
     1681 agatttctca aaataaaaat ttgtatagaa cactcagaaa tgagtgtttt tttattggga
     1741 ttaaattata ttgatacaaa gcttaactat tttaatcatt atataaaatt tgtattaaaa
     1801 aattaagaat aacttggtaa tagtgaaaat ataatgagat tataattgat ttataatata
     1861 actataatga tattgtaaaa atgaaaattg tcctctcaaa attcccaaag agagtaaaag
     1921 tcacttcagt tatagtcaaa aaaatttttc tgtgaaaaga atttagattt gaaaaggagg
     1981 aattaatcat ggaaattgaa actaaaactc cagattggat taatgtacta gaattaaaca
     2041 gtaaaataga tattactggg aaatatgatg taagtaatat gatacaaaat attgtctctg
     2101 ataagtcact agaaggttcc attatatatt ttccaaaagg taattactta tttgagaaag
     2161 gtattaaaat tagtcaacaa ataacacttc aaggagattc ttattatgga ggaggtaatc
     2221 aagtatcaaa tttagataaa aaacaatcaa tcataggaac aaccaatttt ataacccgag
     2281 gagtttctaa tatgagtatt attacattgt caggaacaag tcaatgtatc aaaaatatta
     2341 atttttatta tgatagtcat gatatagaga aaataccgcc taaaaatgta tctgctatta
     2401 cagaatatgg agaaacacaa ggactatctc attttgaaca tctgtttatt tctggatttt
     2461 cagggatagg aattgaaatt ccatactatt caactggtaa tgatattact gttagttcat
     2521 gtggtcttgg tatgaggctt ggagagaagt caatgctttc tagtagcaag atttacgaat
     2581 gtaaaaatgg aatggagatt actactggtg ttagcttaaa taatgtgaga atagaagaaa
     2641 taaaagaaat aggtattaat aataaaggat ttggttttaa cctgattatg aatcttatgg
     2701 tcgaccaatg tggatattgt ggatttatgt ttgaaaaaat gtgctatagc cagataactg
     2761 ccagaattac tagatgcggt cagtattata aaagtgttga ttataatgct tatgagaata
     2821 tggaagatag gaaagaagaa gcttattcca ttttttatgg agatatttta gaaaattgca
     2881 atattgtatt gatgaatagt aatattgata ccttggaaaa tgattcactt gatgaacata
     2941 agatatatgt aatcaaggca aatgaaacaa acaatgtaat gctaacatgt tatgctgaag
     3001 cagataactt tatacaatgt gcaaagggca acttgctgtt agaaaatggt gaaaatactt
     3061 ataaatttta taatggtgag atttgttctg taggaggtgt tcaaatttct gatatagatg
     3121 aaaaagattt aatcaaaata atagagaaca ctttgtatat agatgataaa ggtaagtatt
     3181 taatggttcc tagattaaaa aagaatactg taatatattc agtactagat tctacagaag
     3241 aaataaataa attacatggt ggaatatggg aacagattga tgtaaaagta ttagctgggg
     3301 aatccatgta ctattataaa aagataaaag attaaattat caaactagga ggtagtgatt
     3361 aatggaagag tctataataa ttaaagatat aagtggctgg attaatgtcc ttgattatgg
     3421 aatttgtaat gatgaaacaa aagatgtaag ttttaagttg caagagatta tggatgctgt
     3481 accagaagat tctattatat attttccaaa aggaaaatac ttatttgaaa gtggtgttaa
     3541 gattaagaaa cgattaactt tatgtggaga ttcttatatt ttggcgaatg ttcctaaaaa
     3601 tactgctgga gtaacacaat ttacttttaa aggaaagaaa aatcctaaaa gtgaaaatat
     3661 agcaattata acagcaaaaa gtgtaagaca ctgtattaag agcatttgct tttattcaga
     3721 tagttgtgat agagatatga catctaatca tgaacctcca acagatggaa aacctagatt
     3781 tcatcataat gtaattataa attatagaaa tatatcggct attgtatgtg aagataaaca
     3841 gggagaatca ggtcattata aaaatctatc ttttagtggt ttttctggaa ttgcacttga
     3901 tatgcctaat aattctacag caaatgatat tacaattttt acatccggac ttggaattag
     3961 tacaggagaa aattcaacta ttgctaacag taaggtttgg ggctgtgttg atggaatgat
     4021 aatttctact ggaactttta taaatggtat gagggtagaa gaaataaaaa aagttggaat
     4081 taaaaatatt ggtagaggtt taaattttat tacaaatatc acaattgacc aatgtggata
     4141 ttgtggagtt tggtttgatt ctatatccaa tgttcagata tcaggaaata tcacaaggtg
     4201 tgggcagtac tattatgatg taagctatga taattattta atgttggata atagagttaa
     4261 agaagcatat tctttattct atggtaattt attggaatca agtaatatag ttttatcaaa
     4321 taccaataaa gataattggg aagatttcaa agaagataag cataaggtgt atcttgtaga
     4381 agcacttgaa accaagaatg tactattaac atgcaatata gatgctactg actggattgt
     4441 taagtcagaa aaaggaaatt taacgtacaa taatatgcga caaactgtta tattttacga
     4501 tggaaaacta agtagtatca atggaattgg tatatctgat gaagattatg gggataaagt
     4561 caagataaaa aaaggaaata tatatataaa tagtaaggat aaggatatta agatgcataa
     4621 accagaggta ggtgacatta tgtcaacaat tattaaagat aaaagtattc tttctgctca
     4681 ttatggggga acatgggaga aaattggtga aaaaatagag tttggaataa ctgtacacta
     4741 ttataaattg atagagaaat aagtaaatac gtagtatttg aatagatgta ttttattgga
     4801 gagctaggac tatagtttta atgtaaatgg tttggtaata gcatggattg aataatataa
     4861 aaataatata aaaaattaat ttgattatgt aaaattctat gaagtagtaa aactgtgact
     4921 ttagtcatag gaggttcaaa ctaagtaaaa gggaaaggaa tatataatat tatggaagga
     4981 agaactggga ttaaagatgg aagatggatt aatgtagtag aaagaggtat taaaaatgat
     5041 ggttcgtaca acgtaggtcc tgctttgcag gatattattg atgaaataga aacagatttt
     5101 tgtgtcttat attttccaaa aggtaaatac ctatttacaa gtggtgttaa aattaataaa
     5161 gaaattgtat tgcaaggtga ttctaattca tctacaggca ctacacaatt tataactcgt
     5221 ggagtagaaa atatgagtat tattacattg acagggaaaa aacaatgtat taaaaatata
     5281 aatttttatt ctgatagttg tgagatacag gtaaataaag aaccaccgac aaaaggtaat
     5341 cctaaatatc attatgagat gattattaat aagaatgaaa agaatgttga gatgaataat
     5401 gtatcagcaa tcctgtatga taacagtaag aatatgatta aaggtctagg tcattatgaa
     5461 aatatttata taagtggttt ttcgggtaca ggaattagga taccatatta ctcaattgta
     5521 aatgatatta cagtttcatc ttgtgggctg ggtattgata caggaataga cacaattatt
     5581 tctaatagta gaatatctaa gtgtcaaaat ggaatgagga ttagaactgg aactagtata
     5641 aataatgtaa ggattgaaaa aatacagaaa gttggtctta taagtagtca agttaagggg
     5701 tatgaggggt atggaagtta taaaattaat aatattacga ttgaccaatg tgggtattgt
     5761 ggcttttcat ttgattcaat gaaagatagt tatttttctg gtattataag aaggtgtggt
     5821 cagtattatt ataatacaga ttatgacaca tatttaagta ttaaagatag agtagaagaa
     5881 gcatattctt tattttatgg aaattatttt acagaatgta atattgattt agttagtgat
     5941 aataaggata actgggatga tggcttagaa tataaacata aagtttatgt atttaaaacc
     6001 cttcaaatac caaaattaac attgagatgc aatatagatg cctatgatta tatcgttaaa
     6061 tcggtacatg gaggaggtaa tttaatgtta aaaaataatt ttaacaatta tatatttatt
     6121 gattcatcta atccagaagt tattaatgga attaatttta attatcaaaa gaatggaaac
     6181 ttgttaaggt taaataatgg taaattaagt tataaaaatg gaaaggaaga aacagctata
     6241 ttcagaccta attatggtct tattgtagca tcaacagatt cagaatgtga acaaatagag
     6301 actagctatg gaggaaaatg ggagaagctt ggttctgaag taattggaga agaaacggta
     6361 tattattata aacatcatga accagataaa acaaaccaaa tacaataaaa tttgtattat
     6421 ataatgatat aaagttttaa tatatagtgt ttacctttaa tgtaatattg gtatatttgt
     6481 atagtattaa atttgttaaa aagtaaaatt gtagggtgct tgaaaatagc acctttttta
     6541 gtttcattat aaaattagtt ttgaaagttt aggaataatg tatatttatt tttaaagaat
     6601 tataacctta ttaaggatat tttaaatgct agaaatgaat cagtaaatca tactggaatt
     6661 atcttaaatg ttttgattaa attaagtatt tagagctata attatattaa aaatatatta
     6721 gaacataatt atattactat ggaggttaaa tatgatagta agtgtgagta gaagaactga
     6781 tatacctgct ttctatagta agtggttttt taacagactt aaagaaggtt ttgtttatgt
     6841 aattaatcca atgaatccaa aacaggttag taagatagag ttaaatccac atactgttga
     6901 ttgttttgta ttttggacaa aggatgtaac tccaatgtta tatgatttaa ataaattaaa
     6961 agactataaa tattattttc actatactat aacatcatat ggaaaagaag tagagacatg
     7021 tattttagat aaaagaaagg taatagattc atttaaggaa ttgtctagaa aaataggcaa
     7081 agaaagagta attttaagat acgacccgat tttcttaagt gaaaagtaca cagtagatta
     7141 tcatatcaaa gcatttgcga gtttatgtaa tcagcttgat ggatttacag aaaaatgtat
     7201 aataagtttt atcgatttat ataagaaaac aaaatttaat actaaagcat tacatataaa
     7261 gccaatagaa attacagaaa tagaaacatt atcaaaagag atttctaaga ttgctcataa
     7321 gcatgggata acacttgaaa tgtgttctga agaatataat ttaagtaaat ttgggattgg
     7381 aaaaagtaag tgtattgatg atagattaat atcaaaaatt ataggatgtg aagtagaggt
     7441 aaaaaaagat agtacacaaa gagatatttg tggatgtgtt aaaagtgtcg atataggaca
     7501 atacaatact tgcagacatt attgtctgta ttgttatgct aactttaatt atgcaaaagt
     7561 agaagaaaac tgtaggcttt ataaagaaga taataaatta ctcatagata ctttaagaga
     7621 tgatgcaaaa ataagcatta gagatatgaa gactattaaa gtagataaga ataaacaaat
     7681 tagtatattt gattaaaata aatatactaa ttaccttaat atattaagaa tatagatact
     7741 tttgataaca aaattaaact taatagatat tttctatata agaaagatgg tcataatatg
     7801 tcaatttgac catctttttg atataattaa aacacaaata atttagtaac aaaggaggca
     7861 gaacttgaat ttattaaaaa aatatcctat acctattgca agtttgattt tgtcaatctt
     7921 tactttaggt aacttattac aatcttatag tgaatcatta cgtaatgtaa ttggatttat
     7981 aggatttatt ttgtatgcaa tttatatagt caaaataata ttacttagaa agggtgttaa
     8041 agagcaatta gagaacactt taatatcaag tacatttcca actataacaa tggcaacaat
     8101 gatatttgct acatatataa aaccattatc tttagaatta agtattatga tttggagcat
     8161 aggaattgtg ggtcatcttg tgttaatagt cttatttagt aagaagttct tagttaaatt
     8221 ctcaataaaa acggtatttc catcttggta tatagtatat gttgggatag ttgcagctag
     8281 tgttacggca ccagcattta atcaactttt gattggcaaa attgcttttt ggataggatt
     8341 tgtatcttat atagttctta taccagtaat tttaaagaga gtatggataa tcaaggaaat
     8401 gcctgaacca tctttaccaa gtatagctat attagctgca ccagggtcac tattattgtc
     8461 tggatatatt aattcttttt cagataaaaa taatgtagta ttatacttgt tattcatatt
     8521 atcaattgta ttttatatta tagttttggg atatttaccg aaattattaa gattgccttt
     8581 ttatccgagt tttgctgcat ttacatttcc tatggcaata agtgcaatgg gtatgaaact
     8641 aatgaatggt tatttaacta aatcaggtca ttttataagt tggatttcat atgcttctaa
     8701 gttagaagag ataattgcta cagtgattat tttatatgtt ttagttttgt ttattatata
     8761 tcttagtaaa tctgaaacta catctaaagc taaataagaa ttattaaata tgtgtattta
     8821 ataggaatat tgatataaat atatgaagat aagataaacc atagaaactt aatttagctt
     8881 aattttattg gaggaaaaca aatgaaaaca aaagaagaat atttaaaaga agttagaaac
     8941 atatcagaag aatactttag aaaaggagag tttttttgct ctgaagcagt tctccagaca
     9001 ataaatgatg ctttaggcca acctttaagt ccagagataa ctaagttagc atcaggattt
     9061 ccgataggac ttggaaaggc acaatgtctt tgtggagcaa tatctggggg agagatggct
     9121 ttaggtatag tatatggaag agttcatggt gaagctatga atccaaagat gtttgagcat
     9181 gctaaaggtt tgcatgatta tattaaaaaa gagtatggtg caacatgttg tagagctatt
     9241 acaaagaaat gggatggtga taactttatg tctcccgaaa gaaaagagca ctgtattaaa
     9301 ataacaggtc aagttacaga gtgggttgca gataagctta tagaagatga aaaacttaat
     9361 atcaaatagt gtaatattag ttggtgaatt tataaagtta aatttactta tagtataaga
     9421 tacttgatat tctcgtattg atagaaaaat ttatagcata tgtatttaat gaaatatact
     9481 tatatttaga gtgtggttta atttcataaa tacatatgct attattttaa gtatataagc
     9541 aaatttattt aaataaatag aaacagttaa tttatatgta caaaactata tatgattagt
     9601 gtaaataaat acctatttta taatactcaa cataaagaca ccagacatga ttagtattat
     9661 accaataaaa atagcaagcc attctttttt gttcttcttt tctttaaaaa atataatccc
     9721 tcctaaagtg gctactatca cacaactctg agagatagaa aaacttattg taactcccaa
     9781 tgtattagtc gttatgaaca tacctaaatt tgctattgac cacaatatac cagtaattaa
     9841 attgaataaa acattttccc tatatacaat ctctttagaa aaatacttga caataataga
     9901 tgaagttatc aacataccaa cagattgagg taatataatt ctatatcctt caattaaaaa
     9961 tatttgatta gttacaacat ataatgataa aaatagagat gataataata caactccaac
    10021 acattttaac atatttttat ctgtagattt tttagtttga taatttgtga gaagtatacc
    10081 aagtattata cataatattg ataaagaacc aatcagtaac attttagaac tggtccattc
    10141 attaaacaag attacagcta ataatgtcgt aaaagttaat tgtgaaccat ttgaaattgg
    10201 cattacttta gatacactag caatttgaat tgctttaaac tgcattaatt gtcccaaact
    10261 ccagaatatg ccagaaatca tacttataca aaatgaaaaa aatgtaattt caggatgtat
    10321 aaccagagtt attatagtgg aaaatataaa cgcacttatg gatgtaccta aaagctgttc
    10381 atgaggagaa cttttcttta gatttgcaac tatgggcatc aatccccatc ccattacagg
    10441 aagtaaagca ataattattg tcagcattaa actatttcct ttcatattaa atttagacag
    10501 cttttaattt atcatctaaa attaaagtgt tcaatacata aaaattaccg tatttaatca
    10561 ggaaatatta gtagtttttt agaaaaacat tttccattaa aataaagaca aagggaagga
    10621 gtaaaattga aaatgttaac tgatatgatt caaaatcgtg aaattaaaat aataaagttt
    10681 ttattaaaag aaggtcatac aactgtaaaa gcaatagcag atgatttaga tgtttcagaa
    10741 aaaacagttt caaaatctct taaagaagta gaagcagtgc ttaaaaagat aggtctttct
    10801 ttaataagaa aacctaagat aggtgtttat attgatggag atttaaaaaa aatacttcct
    10861 tacctagata ataaaggaag tcaaattcct acaaccagag aggagagagt tatctatata
    10921 tattcagtac ttttaaagag taatgattat attactatac aacaactttc tgatgaacta
    10981 tatataagtc gtggaactat tgaaagggat ttagcagaag ttgaaaaact gctaaaaaac
    11041 gaagggattt ccatatataa gaaacctagt aagggaataa agttaaatat aagtgaaaga
    11101 gataaaagac aacttactgc taagtttata cataaatttt ggagtagaaa ctggtatatt
    11161 aagcaagagc aagaaagctt ttatcaagca tttgaaaaga tacaagcaga tgtaaatggt
    11221 attttttcgg aagatgattt atcaataatt attgatattc ttaggaattt cagcagagaa
    11281 agaaaatttg agttctctga ttatgaattt cagtcattag ttattcattt ggcaatagct
    11341 gtagagagaa ttagaaaagg cgaatatata gaagaaagtt tgtatactgg taatatagaa
    11401 aataatcaaa tgtcaaatac caattattta gcaaaacaat tagaagaaaa attttcaata
    11461 ttattaccaa aatctgaaat tggatatata aatttacatt tagtagcgtc tgaccaacat
    11521 agagatacta atgaactaaa aaatacttca actaatcctt gtgagacaag tacaaataat
    11581 atatcaaatc ttcgggaaat tataaaaaat acattgtatg agacagctta tgacaaggat
    11641 ttaattgatg gattgacttc acatgtacat tcatcaataa atagactgaa atatggactt
    11701 agtattaaaa atccatatgt aaatacaatc aaacaaaact tttctttatc atttgagcaa
    11761 gctttagatt taaagaaagt aattgaattt atatatgata ttaggataaa tgatgatgaa
    11821 tgtgcttata tagcattaca ttttgaagct tttagagaaa gaatgaaaga acttccacaa
    11881 aatattagag taattctagt atgcagtaca ggattaggtt catctaggtt attgtctgca
    11941 agaataaaaa aatactttcc aatgataaag attgaaaaag tactttccgt acaagaatta
    12001 gtatccagta aaattgatgc agatttagta ataagtacta tttatttaga gttagaaaca
    12061 attccaacca tagtagttag tcctatttta aatgaaagtg atataagaac cttggaaaga
    12121 aatatacaaa atgtttcaag ttgtacttct aagaagtata aatctttcag aaacttaatc
    12181 tttgaagaaa atatattgtt gaatattgaa gcttcaaact atgaagatgc aattaagtat
    12241 ataacagata acctaataaa aaaaggtttt gcaaaagaag gtatagctaa aagtgcaatt
    12301 gagagagaaa atttatctta cacttctttc caatcaatag ctacacctca tgctaatcct
    12361 agttatatta aaaaatctaa tatatctgtt gcaacgttaa aaaatcctat tacatgggga
    12421 aatgaaaagg tggaagttat tttctttatt tcattagaaa atgacaaaaa cctagagctt
    12481 gatgaaatat atgaagtttt ttatgacttt attgacaata aaaaaaatat aaatgacata
    12541 ttaagtgcaa cttccacaaa ggaagtgtac gatttgctag tggaggagag tatatagtga
    12601 aaatatcaga tgtattgaaa aaagagcaaa ttgtttttaa tcttgataca aatacaaagg
    12661 aagaaacaat aaaaaaatta agtagtatat ttgttgaaaa tgggattgtg gacaatgaag
    12721 aagaatatgt taagtctgtt ttggaaagag aagaacattc aactacaggt attggtaatg
    12781 gtattgctat tcctcatggg aaaagtaatg ctgtcaaaaa atcttctata atatttgcaa
    12841 agactaagca taagattgaa tgggatgctt tagatgaaaa accagtagat agtattttct
    12901 tattggctat aagcaatatt gataaggata gcaatcatct tgtactatta tctaaattag
    12961 caactaaact tatggatgat gacaatgtag atgcactaaa attagccaat tcagaacaag
    13021 aaataatcaa tatatttagt gaagaaggag agatgtagaa atgaaaagaa aaattatagc
    13081 agttacagca tgtgcaacag gagttgcaca tacatatatg gcagctcaag cattaaagaa
    13141 agcttcaaaa aagatgggtc accttataaa agttgaaaca caaggagcta caggaatcga
    13201 taatgagtta actacaaagg atgcacaaat tgcagaagta gtaatatttg cagtagatac
    13261 taaagtcaga aatgctgaac gttttgaggg aaaagaaata ctagaagtac cagttaatgc
    13321 acctataaag gatgcagaga aagtaataaa tgatgcatta aagttaattg acactaaata
    13381 aatggaggga aatgtaatgt tgaaaaagat agtctctgag ttaaaaaagc atgtcttaac
    13441 tggaatctca tatatgattc cattagtaat tgcaggtgct atgataatgg cgatatctcg
    13501 tgtaggtggt tctttctata atataccaga tatatgggat gctaaatatg cagaaagtgc
    13561 tagtagtatt gttagattat tacatgattt agatgggttt ggaggtactg cattaggatt
    13621 aatgtttcct gtaattgctg catttatagg attttctata gctgataaat tagctattgt
    13681 accaggtctt gttggaggta tgattgctaa agatataggt gcaggatttt taggagcttt
    13741 ggcagttggt cttatagcag gatatacatg tttgtttatt aaaaatcatg ttaaattacc
    13801 aaaatcagca gcttcaatag taccaatatt tatagtacca gtatttggga cattaatcac
    13861 tgttgtatta atcaattatg tgataggtat accttttgcc acattaaata ctggattaga
    13921 aaattggtta aatggattat ctggtacaaa tcagatacta atggcagcta ttataggggc
    13981 tatgatggca gttgacttag gtggaccagt taataaagca gctttaacaa catcacttgc
    14041 attattaaca agtggaattt atgcgcctaa cactgctgca atggtaggaa tagttatacc
    14101 accactagga ttgggacttg ctactatact agcaaaacaa aaatataata aacagttacg
    14161 tgaagctgga aaatcatctc ttataatggg tttaattgga gtaagtgaag gagcaattcc
    14221 ttttgcggta gaatctccat taaaagtaat accttcatgt gtaattggaa cagcaattgc
    14281 ttcagctatg gcagttggtt taggttcagt aaatgcaact ccaattagtg gattttatgg
    14341 atggtttaca gtagagaatt ggccaatgta tgtgttatca atagcagttg gaacagtaat
    14401 tgtagctgga ttagttatag tattgcgtgg aaatccagtg attgaggatg atgaatttga
    14461 agatgatgat tttgatgaag cagagtggga atcttaaaat ttaataacta tttaagacgt
    14521 gttataagct atattcaata tgaatcttat actaaataaa gtgttaaata ttcagtttag
    14581 acttaaatat aaaagataaa gaacagagga gaataaatga taaaagcaca tattgttaat
    14641 catacacact gggatagaga atggtatttt acttctggag atgcattagt acttagtgaa
    14701 caactattta cagatgtaat tgatgaatta gaacgtaatc cagatgtaag ttttgtactt
    14761 gatggacaac tttctatttt ggatgattat gtacaattac acaaagaaaa aatagaaatc
    14821 attaaaaagt taataaaaga agatagacta catattggac catggtttac tcaaactgat
    14881 gcattctttg ctagaggaga gtctatttta agaaacttaa tgattggaat ttttgaaagt
    14941 aaaaagtatg gtaagtatat gaaaattggg tatttgccag atacatttgg atttaatgca
    15001 cagatgccta tactattaaa gcatgtaggt cttgataata tattcttttg gagaggtata
    15061 catcttggta aacaggttca atcaccatat tttaagtgga aaagcttaaa tggagaaagt
    15121 tatatatatg cagtaaatat gccacatgga tatggaactg gtatgctttt agagccttca
    15181 ttaaagtatg ttaatggacg cttagaccca gctattgatt ttattaaatc atatactgat
    15241 gtagatgagg tgttaatacc ttctgggaat gaccagttaa atataatagg tgatttcaaa
    15301 aataaaatta aagcgattaa tgatatcgga aagtattcat atataacgag ttcatatcaa
    15361 gagtttttaa aatatataaa gtccatagaa tcactagaaa gctatagagg tgaatttaga
    15421 gaacctgtat tagcaagaat acataaatca attggttcta gtagaatgga tataaaatta
    15481 gcatgtgata aactagaaaa caagctgatt aaaagaatag agccattatt agtaattgct
    15541 aaaaagagca aaattgaaat aagcaatcag ttacttataa atacatggaa aaaacttttg
    15601 gaaggacaag cacacgatag tttagctgga tgtgtaactg atactgtaac agatgatatt
    15661 cttcaccgta taagagaagc aaatgaaata tgtgacagta ttgaaaatac tattgtaaaa
    15721 aagatttcag aagggcttaa attatctaaa aatgatattt tagtatttaa tacagaagct
    15781 aagcgtttta atggatataa agagatacaa gttgtaagtg atagtaaaaa tatttatttt
    15841 aaagataatt tagatgctac tataattgaa gaaacttatg taaaaccgag agaaaatgta
    15901 ttagaagaaa cacctgctgg taatatattt attgaagaac caggatatta tatattaaaa
    15961 gtaagaataa atgtacagct tcctgcactt ggttataaag ttgttagttt tgaattaagt
    16021 gataaagaaa tggtgtcact tgataaatct aatgacactt ttatatctaa tgataattat
    16081 aagataatat atgaagatgg aagcttaaat ttacaactta aagatggaac ttacatatca
    16141 aactttttaa cattaaaaga tagtggtaat gcaggtgata cctatgactt ttctccacta
    16201 aaaaatgatg agaatataaa actttcgttt gataaagtgt atacagaaaa agctttagga
    16261 tatgaaaaaa tggtggttaa aggtactact ttactaccac ttacattaga agatagaaaa
    16321 aacaaatatc ttaatggtaa attaaatgta aaagtaagta ttgtcttatc taaagaaaac
    16381 ccattgatgg atgtaaaact ttgtgttgat aacacgatat ataatcatag actacgtgtt
    16441 catataaaaa ctgatataaa agataataaa aacatagcat cattgccatt tggatatata
    16501 acaagaaaaa atggagtttt agacaactgg gaagatattt acagtgaaat gcctatagat
    16561 ttagaacctt tggaaagtaa tattacgctt acaaaccaaa atagaagttg tactgtattt
    16621 actagaggaa taaaagagta tcaacatatt gaagatgaaa ttgcattgac cttacttgct
    16681 actacagatg aactaggaaa gcctgattta ttatatagac caggacgtgc ttctggtgat
    16741 acaactaaaa aaggtcatat aagaattaaa acagataaag ctcaaatgct taaggaatta
    16801 gaattttcat ttgcaatata tatggattct aaatcatttg atgaattgga aattgcgaat
    16861 ttaacccaca attatctaag ggaatgtgta aattatcaat tacaggatta caatttcttt
    16921 ttatatcata tagataataa gattcaaaaa tcaattgtca ataaaactgt aagtagagaa
    16981 ttagaaataa tatctttacc agaaaactat cttatatcag catgttatcc ttcttattac
    17041 agtaaagata aatttataat aagagtggaa aatccaagta gtaatagaat gatattggat
    17101 gagcaaatat ttaaaaatag aaatgggaaa attgtaaatg caatagaaga tgtagaagag
    17161 gaacaggtat atgaaatagc accttttgac gttatttcaa tattattgga tttataagtg
    17221 taaaagtaag tttattgttt aaagtctgac agatattaat ctgccagact ttttaatttg
    17281 tataaatttg ggtaaatatt aatgaatggt tttagtattt aagtatgaat ttagtattta
    17341 atatagaatt tattgaagta gaataattag tttaacagga taagcagttt aaataaagaa
    17401 ttatta
//
LOCUS       DAMJWU010000075        17345 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMJWU010000075 DAMJWU010000000
VERSION     DAMJWU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.076"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     misc_binding    225..456
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            564..2045
                     /locus_tag="PC460_002626"
     CDS             564..2045
                     /locus_tag="PC460_002626"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF2601506.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2055..2948
                     /locus_tag="PC460_002627"
     CDS             2055..2948
                     /locus_tag="PC460_002627"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF2601507.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3072..4133
                     /locus_tag="PC460_002628"
     CDS             3072..4133
                     /locus_tag="PC460_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF2601508.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4288..4800)
                     /locus_tag="PC460_002629"
     CDS             complement(4288..4800)
                     /locus_tag="PC460_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF2601509.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            5320..6255
                     /locus_tag="PC460_002630"
     CDS             5320..6255
                     /locus_tag="PC460_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF2601510.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(6347..8218)
                     /locus_tag="PC460_002631"
     CDS             complement(6347..8218)
                     /locus_tag="PC460_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF2601511.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            8408..9334
                     /locus_tag="PC460_002632"
     CDS             8408..9334
                     /locus_tag="PC460_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2601512.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9473..9619
                     /locus_tag="PC460_002633"
     CDS             9473..9619
                     /locus_tag="PC460_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601513.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(9724..10248)
                     /locus_tag="PC460_002634"
     CDS             complement(9724..10248)
                     /locus_tag="PC460_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2601514.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(10301..10525)
                     /locus_tag="PC460_002635"
     CDS             complement(10301..10525)
                     /locus_tag="PC460_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601515.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            10722..11846
                     /locus_tag="PC460_002636"
     CDS             10722..11846
                     /locus_tag="PC460_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HDF2601516.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANSVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(12320..13144)
                     /locus_tag="PC460_002637"
     CDS             complement(12320..13144)
                     /locus_tag="PC460_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601517.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(13492..14547)
                     /gene="ispG"
                     /locus_tag="PC460_002638"
                     /gene_synonym="gcpE"
     CDS             complement(13492..14547)
                     /gene="ispG"
                     /locus_tag="PC460_002638"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF2601518.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(14729..15733)
                     /gene="rseP"
                     /locus_tag="PC460_002639"
     CDS             complement(14729..15733)
                     /gene="rseP"
                     /locus_tag="PC460_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF2601519.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(15746..16900)
                     /locus_tag="PC460_002640"
     CDS             complement(15746..16900)
                     /locus_tag="PC460_002640"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF2601520.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(17064..17267)
                     /locus_tag="PC460_002641"
     CDS             complement(17064..17267)
                     /locus_tag="PC460_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601521.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKSI"
BASE COUNT         5967 a         2701 c         2053 g         6624 t
ORIGIN      
        1 acagatattt aatagttatc ttttgattac tttcattaaa aacagcttca tcactatgaa
       61 aatctaggtt tttaaacttt taaaaaagtt aaaaaaatat ttttctaata ctcaatttga
      121 ttacaatttt tttacaattt aaaaaatatt ttttccaaat ttttatatta atacttgact
      181 ttgtctgttt tataaactag aataattaat aatattaaga atattgaaag gcattgatta
      241 ggaagagtaa atctgaatcg attaccagag aaggaaacta ctagctgaaa tgtttcccta
      301 attagaagga ttgaaaacta cctaggagct tctatccgaa gttatcttaa tgattttagt
      361 aggaatagac gtttttatta cttgcgttaa agtatcaagt ggatttttta aaatcaattt
      421 gggtggaacc gcgggaaaca gattctcgtc ccttttttag ggatgagggt ctttttttat
      481 tacctaaatt cagtttttaa aatattctgt atctaattta attagctaat taaaaattta
      541 aaatatatgg aggattataa aaaatgtatt attcaagtac gagaggcacc gaagaaaaag
      601 taactgcatc acaagcaata ataaaaggaa tatcaaatga tggtggtctt tatgttccaa
      661 gtgaatttcc caatgtaaaa aatgaactta taaatttagt aaatttaacg tattctcaga
      721 tagcattttt tgtacttagt aaatttttat gtgattttac tgaagatgaa attaaaaatt
      781 gtatagaaaa tgcatatgat gaaaagtttg attgtagtag tatagctcca ttaaataaag
      841 taaatgatac ttatttctta gaactatatc acggtccaac tttagcattt aaagatatgg
      901 cacttactat aatgcctcat cttcttaaaa catctataaa gaaagataat ttagagaaag
      961 atgttgtgat tcttactgct acatctggag atacaggtaa agctgcactt gagggattta
     1021 aagatataga taaaataaag ataattgtat tcttcccaga agatggtgta agcccagtac
     1081 aaaaacttca aatgaaaact caaactggaa aaaatacata tgtagttggt ataaaaggaa
     1141 attttgatga tgcacaatca ggtgtaaaga agattttttc tgataaagaa tttaatcaaa
     1201 aactattgga taataattat atattatctt ctgcaaattc aattaatata ggaagacttc
     1261 ttccacaagt tgcatactat ttttattcat atatgaaatt agttagcaat agtgatataa
     1321 atttaaatga taaaataaat tttgttgtcc caactggtaa ttttggtaat attctagctg
     1381 gatattatgc caaacaaatg ggtcttccaa taaacaaatt aatatgtgca tcaaatgata
     1441 ataatgttct ttatgatttc tttaaaactg gtgtatatga taaaaataga acattgaaat
     1501 taactacttc tccttctatg gatatattaa tttcaagtaa tttagaacga ttactgtttg
     1561 aaatatcaaa tagaaatact gatattgtaa ataagctttt atcagacctt agtgataaag
     1621 gagtctacaa aataagtgat gatatggaaa aaaatttagc ctcattctat ggagaatatt
     1681 caaatgaaga agaagttaaa agcacgataa acaatgtgtt taaaaattat aattatttga
     1741 tagatactca tactgctgtt gcatacaatt gttatgaaaa atataaaaaa gaaacttctg
     1801 ataacacaaa aactgttatt gtaagtactg caagtccatt caagttttct gaagatgttc
     1861 taaagtctat agattgtgat tttaaagacc ttgatgactt tgctataatt gatagattat
     1921 caagtatttc taaaatagat attccaaaac ctattaaaaa tcttaaaaat gcagaaatac
     1981 tacataaaga tatttatgaa aaagatgaat taaaactagc tataaaaaaa ttcttaaagg
     2041 tataggtgat tattatgtta gagattatag ttcctgcaac aagtgctaat ataggtccag
     2101 gatttgattg tcttggaatt gcgttaaata tatacaataa attttatgtt gaagaaattg
     2161 aaagtggtct tgaaatagaa ggttgtgaag atgcttacaa aaacgaaaac aacttagttt
     2221 atacatctat gaagtacttt tttgatagag ttaaaccaga aaaaatacct gctggtatca
     2281 aaataaaaat ccaaagtgaa gtacctatat gtagaggtct tggcagtagt gcctcttgta
     2341 tagtagctgg agttatagct gcaaatgctc tatcaggtgc taatttagat aaaaatcaat
     2401 tactaaatat agcttcagaa atagaaggtc acccagataa tgttgctcct gctattttag
     2461 gaaatatgat tgtatctgtt actgataatg agaatataca ttatgatatt ataaaaattc
     2521 ctgaagaact taaattttgt gcaatgatac ctaattttaa gttatcaaca gaaaaagcta
     2581 gaggtgtttt accaaaggaa ataccatatt ctgatggggt attcaatgtt agtagagttg
     2641 ctttactaat atctgcctta ctaaacaaaa actttgacct acttaaagta gcttgtcaag
     2701 acaaattgca tcaagattat agaggaactc taattgagaa ctacaatgat attgttgaaa
     2761 aatctgaaca attaaatagt attggagtat ttttaagtgg agctggacct actataatgt
     2821 ctttaataaa agaaaatgat gatagttttg tggataatat gaaaaattac ctacaaaaac
     2881 taaaatctga ttgggaaata aaagagttat gttgcgattc taatggagct gttttaaata
     2941 taatataatt atcgtcttct atacaaaata taactactct tttattataa tttgtagtat
     3001 aataattagg actaggtaat aatattaagt attataatct agttctttaa acatgtttac
     3061 attgaggagg gattatcata aaagagttaa taattagaga tttagttgca aaagttccta
     3121 taattcaagg tggaatgggc gttggtatat ctaggtcatc actagctggt gcagttgcta
     3181 agcttggtgg tgttggtgta atttctggag ttcaaattgg atatgatgag gaagattttg
     3241 aaactaatac tataaatgca aatttaagag ctataaaaaa acatatttct aaggccaaag
     3301 aaatctcaaa tggtggtatt attggtatta attttatggt agctatgaaa gaatatgaaa
     3361 catacgtaaa ggaagctgtt aaagctggtg tagaccttat aatatctgga gctggattac
     3421 ctaataagct accatcatta gtaaagggaa gtaatgtaaa aatagcaccc atagtttcaa
     3481 ctgcaaaagc tgctaatgta attttaaaaa tgtgggatag aaaagagaaa actactgctg
     3541 acttaatagt tgttgaaggt ccaaaagctg gtggtcacct tggatactct aacgaggaac
     3601 ttgataacat agattctata gactatgata aggaatttgt tgaaatttta aaggttgcaa
     3661 atacttatgg agaaaaattt ggtagaaaca taccagtagt tgctgctggt ggtataactt
     3721 ctagtagtga tgttaaaaaa tatatagata tgggtgcaag tggtgtacaa gttggtacaa
     3781 gatttgttgc tacttacgaa tgtgatgccc atgagaactt taaaatggca tacataaatg
     3841 ctagtgaaga agatgttcat atagttaaaa gtcctgtagg tcttcctgga cgtgccataa
     3901 gaaacaaatt tatagaagaa gttaaaataa atcgtccaga aataaagaag tgctataatt
     3961 gtttgattcc ttgtaatcca aaagaaactc cttactgtat ttctcaagct ctaatcaatg
     4021 cagttaaagg agatgttgaa aattcacttt tattttgtgg aaatgatgcc tataaaattg
     4081 ataaattaag tactgttgaa gatgttatca atgaattgat ttctgaatta taatttaaag
     4141 aaaactaaat taaaatttta tcaagctaag gagtttaaat acgttattta ttaatcttta
     4201 taaacatatt tatgtcctta gcttttttgt gtcaatttcc ttttattgaa attttaattt
     4261 aattagagat agtattaacc cttataacta atcttctttt acaagtacat tggttatttc
     4321 agctatatat aaagtatgat aatctttttg tggataccat cttccatcta taccttcagc
     4381 aataaaacat tggggttcaa gtttctgact atacattttt ttgcatataa tagccatttt
     4441 agcttcctta aaataaggtg tatcatttaa gtgttctata ttaaatccaa tctgtgatat
     4501 cttatcctct tctctaccag atacttttcc acaatatgaa agctctttac gaaaatcttc
     4561 atcaaaaaat gtaagagaaa atttatctgt attatctata aattccttag tataacgctg
     4621 tggtcttatt actacaaaag caacatcttt tccccacata actccaagcc ctccccagct
     4681 agcagtcatt gtattcactt taccttcatt ttctgctgtt ataagtgtcc aatctttacc
     4741 tatcaatcca aatggattat ttttaatctc ttctgccaaa atttctttaa aatcactcat
     4801 atctctaata acctctcatt ctcttatatt tatattcctt ttattttcat gataaattat
     4861 aaatgaagtc tagttttgaa aataaaatac ttttttaaat atattattgc aaataagtaa
     4921 atatatatat acactcttat tttttaatat atttaataat ttcactcaga atatatatat
     4981 tctttactca aattaaatat aacttatata ctttatgtgt aaattatggt aaaaatatta
     5041 tttaatcaaa tcaaaacttg atttatatac ctggaaaagg tattatatta aagagtgctt
     5101 aaaatacgca tttcataaat agctatatat aaaataataa ataaggagag tgatgggcaa
     5161 tggatactag tccgttgcgg agagaatttt ttacattttc atcacttaat aatatctaaa
     5221 atcaaaacta atacatatat tcagttatat aaattagtta attaatagat actattaaat
     5281 gatgagattt atataaaact gaataaagga tgtgttgcca tgataagata ttacaaaact
     5341 atagattcaa aattagagaa acttagtttt tttgaagatg gctgttggat aaatcttgtt
     5401 gaacctaatc atagtgaaat caacgaaatt tctaatttat taaatataga tgtagaaagc
     5461 atagaatctg cactagatga agaagaacgc tcaagaatag atgttgaaga caatcatact
     5521 cttatactta tagatatacc agtagacgaa agtgactcta attcttctca ttatactaca
     5581 atacccttag gtattatact aactgaagaa gctattgtaa cagtttgtga tgcacaaact
     5641 aagattttaa atgactttat agttggtcat attaaagatt tttttacgtt taaaaagact
     5701 cgctttttgc ttcaaattct tcacaaaaat gcagcttatt acttgcatta cttaagaaaa
     5761 ataaataaaa tgacaatcat aatagagcga gaaatttaca aatcaatgaa aaacaaagaa
     5821 ttggttcaat tattggaatt agaaaaatct ttggtatact tctctacctc actaaaatct
     5881 aatgaacttg ttttaaacaa aatggttaga actgctggta taaagaagta tccagatgat
     5941 gaagacctat tagaagatgt tatagttgaa aatagacagg ctcttgatat ggctaaaata
     6001 tatggagata tattgagtag aattatggat gctttcagtg caattataag taataatcag
     6061 aataatgtta tgcaaatttt aacagtagtt acactaattt tctcaatacc tacaataata
     6121 tcaggattct ttggtatgaa tgtcatcaac atgccatttt ctaataaccc aaacggattt
     6181 tggataatac ttcttattac ctctataata tgtatagtga ttacattttt catgtcaaga
     6241 aataagctat tataaaatat taataaaaaa gccattagaa ttaattgatt tacatttatc
     6301 ctaatggctt ttatatattt aaatctatat aatcttataa tactttttaa ctatcatgtt
     6361 cttcatctat aatcatttca gtttcaagcc cttctagcat tgaatctaaa ctttctttta
     6421 tatttagtat ataaattggt acatactctt cttcatctat tacctcttct tgcaacttaa
     6481 atggaggaat taatacttca tcaaagtcta tatttaaatt tggaaagcct tctactatct
     6541 cttcacaatt atcgtactct tcatttgcta aagaaatgta catatctagg ctctttttat
     6601 aataaagacc tgcaagcata tactcacact tcttacttcc atatttaata ccttctttat
     6661 aatatttctc agcttctgcg tcatcattta attttaaata aatttgacct aaattatata
     6721 ttgattcaac acaaccttgt gctctagcct tttcatacca aaatttagaa tttacataat
     6781 ctttatagaa catatcataa attaatccta ccatatgttg agcatacaca ttattttcat
     6841 tggcaggtgt atcaaacatc attttagact cttccaattt accttctcta aaataaattc
     6901 tacctagatg tatacgagca ttcatatggt ttttgctact tgccatttca aaatactttc
     6961 tagcattttt tgtatttcct aagctctcat atatacaccc caatctataa ctagacctcg
     7021 tatctccatt ttcagatgct ttcttatacc aacttattgc ttcttcaata ttatttaaat
     7081 cttcatagat atttcctaat ttgatttgac aagatacatt atttggaatc ctcgaataat
     7141 acgaaatcgc tttttctata tcttgattct gataatataa atcccctaga ttttcaaggg
     7201 aacttttgca tccaacagct atagcatcct cataatattt aatagcattt tcataatctt
     7261 tttcttcaaa atatatacct gccagattat tttttgcttc tgttatatca tcttcagcag
     7321 ccattttata ataagttttt gccttctcta tctcattttc tctattatat aaattagcaa
     7381 gtctatactt agaaagtgta tgattttttt ctattgcttt ctcataataa taaattgcat
     7441 ctcccagctc ttcttttcta tcatatacat atgcaagcct atactcagac ttcacatcat
     7501 ctctatcagc actcttttta taatacttct ctgcatcttc taaattatca tttatttcca
     7561 taatcatacc caagttaaag caacctttca tatgacctga cttgtacaac tgtttatata
     7621 aacttactgt tgatttatta tctttctttt ttccatttaa tattgctaaa ttatattttg
     7681 catcatcatc taaaagcaca gaaccttttt tatagtattc atatgcttca tcatctttct
     7741 ccaaaaaagt atataatacc cctaaattaa aacaagcttt acctaaattc ttgtctgctg
     7801 ctgtttttaa ccatttttca caccttacaa aatctttcat tttaaaatgg catacaccta
     7861 aattattttg tgcttgtaaa taacctaaat ttgctatctt ttcataccaa tatatagcat
     7921 attcatagcc atctaactta tagtacatat tacctaaatc aaaagcagca tcttcacttc
     7981 cacttttata tgccattgaa taccactttt ctgcttcttt aaattgtttt ttactttcat
     8041 atatatttcc taaagtatat tgagatttta cgtaattatc caatgcagca ctcttataca
     8101 actctattgc gctttccatc ttacccatct tttcaaggag ggcaccctta gtatgtacga
     8161 ctttcgggtc attagatata ttttttattt tttttataga aaattttgta tttttcatta
     8221 ttcgctacct acccatttta taaaatatat tctctcaaac attattatac attataatct
     8281 acaattaatg ctataatatt gaatttttac cttttgtcac tatattgtaa cattaaattt
     8341 taaattttat aatcattaga ttttattatg taattggtat ataatgtata tatagggggg
     8401 acattttatg aatttcagca atatttttat ccaagttgca gttttattta taattatttt
     8461 agttggatat tttgtcagaa aattcaactt attagatgac cactgcacat ctaagctttc
     8521 aactttgaca atgacagttt ttttaccatc aatgataatt agctccatgc aaattaactt
     8581 tgatagtaaa atgatacaaa aaatactatt gttattattt acatctttga ttatgtatat
     8641 agtttcaatt ataattgcat ttttattgaa atacatttta aaatgtaatg ataaaaaaga
     8701 tttgggaata taccagtata tagttgtttt ttcaaatgtt gcttttatgg gttatcctgt
     8761 aattgaagca gttttaggtc atgaagcaat attctatact gcaatattca atttgccttt
     8821 taacttattt tctttcactt taggaattta tcttttatct aaaggcagta caagtagagg
     8881 tttttcaata aaatcactta taagccctgc tacaatagct attgttattg gacttttttt
     8941 atttgtaaca ggacttaggt taccccaatt tataaacgag ccacttgaga tgttgggaag
     9001 tataactaca ccaatatcca tgattatcat tggaagctta cttgctaatt cttcagctct
     9061 cgattgtttt gtcaacaaaa aattatatat agtcactttt ataagattat tggtacttcc
     9121 tgtgattgtg tattttattc taaaaggatg gataaatgat aagatgattt tagctatacc
     9181 agtagtaatt tcttcaatgc ctgctgctgc aaatactgct ataatggcta atcaatatga
     9241 ctccaatata acactagctt cacaatgtgt gttttttaca acactatttt cagttatatc
     9301 tatacctttt ataagtattt tactcttatc ttgatttatt tttttatgta ttatataata
     9361 acatattcaa taaattacaa atataaacat tttattgtag tatattgatg gaattttttc
     9421 ctagatattt gtcaataata tctatatgta ttatagaata ggaggcttaa atatggatat
     9481 ggatttactc tttaaaatag tatttgctat agttgctata ggtgtttctg taaaaatcat
     9541 taaaactgtt gctagtttag cttttaaaat agcgttgata ttgctaataa ttttatttgt
     9601 atatagaatg tttttgtaaa catttaaaaa taagtttttt aacacaatag agaagtaaaa
     9661 tagattatct ttaatgtaaa ttttttatta aagacactct attttacttc tctattagct
     9721 tatttaaatt aaatttttat tatatcttcc cataatataa acatcttcaa attttccatc
     9781 tctgattaca gcatattttt tagttccctc tatttcaaaa cctagctttt tatataaatt
     9841 taatcccttt tcattatcca caattacttc taatcctact ctagttaaca taatccaatt
     9901 atctgctata tctaaaagtt gttccataag cttcttacct actcctcttc cctgataatt
     9961 ctctgcaaca cttattccta tttctgcgct atgtctcaat cgtggagaag aatttacatg
    10021 taacccagca aaacctatta catctttttc tccattatta ttagtttcag ctacaaacat
    10081 ataatcattt tctcccaaag aagctaacat agcttcagtt ttatcgactc tgtcactcat
    10141 taatgccaaa gtatttctca taacactgta tgttcttcta atttcattaa aagcttgtgc
    10201 atcttctctt cttataggtc ttattgttat atctataggc ttactcatat tggattcccc
    10261 ctcaaacata atctttattt ttaattaaaa ttttacatct ttattttata ctgttcaaat
    10321 ctatgtatgc ttctacctga ggtacatata tcatattatc ttttatatgt gtacttccaa
    10381 attcttttaa ttctgtacct tctttatcct tagttatttt atatctaatg gattccatac
    10441 cttcaactac ttcagcatga aaaaacatac cttcatattg tttaaatcta aaattaaaat
    10501 tgtctttagt aagttttatt atcatatttt tatctactcc ttatataatt gatatttttc
    10561 ttattattag ttgtatatat actgcttatt catattaata tattataact tatcatttat
    10621 ttagtataaa gttataaata gatatattta tacttatttt acaatctttc ttcaattgat
    10681 atataatatg tttatataaa ctaatgtatg tgaggtgaaa tatgagcaaa aaaacaaaat
    10741 atttttcttt aattctcttt cctattgctt tgcttttaaa ttttatagcc agcaaagttc
    10801 caaatattgt tgaaaaatat tactcccaat tcatagataa aataattgta caaatattga
    10861 gtaaagtttc tggaattttc ccattttctc tctatgagat tacaatgtac ataatagtta
    10921 tatctatctt cttattttta tgttgtactt tatctacaat ttttaataag aaaaaaaaat
    10981 taaacatata tttaaaaaat tccattttaa atatattatc cattgtatca atagtctact
    11041 ttctttttgt agtgctttgg ggaataaact ataataggat tccactagaa accacactta
    11101 taaataacta taatttaaaa aacaatacct ctatccaatc aaaacaatat agtgttaaag
    11161 agcttactaa attatataaa ttccttgtaa ttaaagcaaa tgaaactaga aaactcacat
    11221 tacaagataa aaatggtatt gtaaaatcta atacagatta taatggtgtc ataaatagag
    11281 ctcaactagg atacgataat atcttggata tattacctgg tgttagtggt agttattcta
    11341 agcctaagta tattatttca tcaaatctaa tgtgctatac aggaataact ggtatatact
    11401 ttccttttac aggtgaggca aatgtaaata tagcaatacc tgacttatat attccttgta
    11461 cagttgaaca tgaaatggca catcaaaggg gatttgctag tgaagatgag gctaatttta
    11521 tagcgtatct tacttctata aaacacccca atattgattt taactattct ggatatattt
    11581 tagctttaaa ttatactgct tctgctttaa gtaaggttga ttataatgca tatgttgata
    11641 tttctgctgg catttcagat tctgtccgta gagatttaaa aaatgaaagt gaattttggc
    11701 aaaaatatga aggtaaaatt aatgaaattt ctaatgagtt taacaattct tacttaaaag
    11761 ctaatagcgt tactgaaggt actcagagtt atggtaaaat ggttgattta ttgctaactt
    11821 attatgaatt atatccatat aactaaacac tacaattcaa aattttattc atatacagct
    11881 gatattaaat cagctgtttt ctatttaaat aaattattta acttcaagca atcttatata
    11941 atttgatagt acttatacta tctgtataac atttcaattc taactatttt agttaaaaca
    12001 tttctattat cttttttaat aaatgtttta actaaaattt ttaaaacata aaacttccac
    12061 attatttcac tatctatata aaaatctaaa aattcacata taaaattaca taactaaaaa
    12121 atcataaatt tatagaaaac atatattcaa aataaaaaag catgtatcaa aacttgacac
    12181 atgcttataa tcttatgtat atatatcatt ttgtcacaat aaaaatttac ttgtaacact
    12241 gatattttta aacaaaatag tccttattta attaaattaa cattaattaa attcgtactc
    12301 attttgctat cattaaacat tacttatagt aattttctaa tacactattt gaaacttcct
    12361 tcccttgaaa acttttcaac caattctcat atgctgagtt tttccataca ctaccatcat
    12421 taaatgttac ttgttttaca caagatataa tgtactttac accatgctct ccagttgatt
    12481 gtttccattt atcaaacaat agaaaggcat cattttgatt cttacctggt gctattgcat
    12541 tttgctttgc atattctttc tccatttgtt tccattcttt caaccaattt tctacccatg
    12601 ccttgccttg ttgtttttca aacatactta ttatatcctg tggtggtgat tgttgcattt
    12661 ttctataagt atgtgaatat gattttggag acacaggaga tatgcctgta acaataccat
    12721 agtctacacc agctgggcta aatccaacac ttcctacttc gccatctgct gccacatttt
    12781 gagcatccca atatagctca agtggttttc catttttatc atatgctaag catgtaattt
    12841 cataatccat aattgccttt tcactgctat ttgttaagct tataacattg ctaggccaat
    12901 gatatggagt atcatcatac tgtattttgc tattatttat aaataatgga atattctcat
    12961 tataaacaag acctttacta ctttgaatat ctgatacctt ctttgtgaca gtacttaccg
    13021 tatttgctaa gtatacattt gttttttctg aattggtcgt actaagagaa aatgttgttg
    13081 tcgttccagt aatcagcaaa gcaacaacta aaataggtaa gctagatatt tttttatatc
    13141 tcacaattct tcacctcttt ttctattaac ttataataaa acaatttacc aaaacattaa
    13201 aatctatgtt aacataattt accaactgtt ctagtaacta tatcctatca tagttactat
    13261 caagtttcaa tactattgat ggaaattttc actatattaa atcttttttt aattttataa
    13321 atttacaata tgatttaact catacatata tagtcaaatt agaatataag tttcttacat
    13381 ttaatgaagt ttataaaata aaaaatgact agataattaa atactgtaaa taaaaactta
    13441 ttacaaaagt atttaaaaat ctagtcaatt aatatatata tgaattgtat actataattt
    13501 ttcaatctct tctagcaact catcaactaa tttgttacca tcaatcattc taactatttc
    13561 acccttttta aataaaagac cttcaccttt acccccagca atacctatat ctgcctctct
    13621 ggcttcacca ggtccattta cagcacagcc cataattgca acagtgatat ttttttccat
    13681 actaccaatc ttttcttcaa cctcatttac aacactaatt aagtctatat tacatctacc
    13741 acatgtaggg catgatataa ctttaatctt atcatttaaa agtcccaaac ttcttaaaat
    13801 ctgtttacca acaataacct cttcaacagg gtcgcctgtt aaagatattc taacagtatc
    13861 tccaattcct ttaagaagaa gtgctcctac ccctatagaa gactttattg ttcctttgtg
    13921 aacacttcca gattctgtaa ttccaatatg aagaggataa tcaacttttt ttgatattaa
    13981 ttcatatgca tcaacagttt tataaatatc ggatgatttt agagatataa ctatattatg
    14041 aaaatctaag tcttcaagaa tcttaatatg tcccattgca ctttcaacta atgcttcagc
    14101 agttgcagag ccatactttt ttaaaagttc tttctctaaa gaacctccat taactccaat
    14161 tctaattttt aaatttcttt ccttgcattt ctcaactacc atcttaactc tctctatact
    14221 tccaatatta ccaggattta ttcttattcc atccacacct tgttctatag cttcaagggc
    14281 tagcttataa tcgaaatgta tatctgcaat aattggtata tttacactag actttatctt
    14341 tgctatattt ttagcagctt taatatcagg aacagcaact ctaactatat cacaaccagc
    14401 ttcctctaat ctctttattt gagctattgt agcatctgca tctcttgtat cagtatttgt
    14461 catagattgt atacttatag gattttctcc tcctattttt acagttccta cacttacttc
    14521 tctcgtcttt cttctctcgt aactcatatt ccacctctat atattatttt atattgaatc
    14581 tctttaaata ttaaaatatt ataacctaca tgtatattat aaattaaaaa gctatctagt
    14641 taaataagaa taaatttcat atataaaaat taatttattc ttatttaaaa ctacatagcc
    14701 tattttctta aaatctctaa aaactaaatc attgaaataa tcttagtata tctttatatg
    14761 tgataaaaag cataaataac attaaagctc caaaaccaac aacatttatc atgccttctt
    14821 tatttgggtc gagtttttta ccacctctaa cagcttctag taacagcata agtattctcc
    14881 atccatcaag tgctggtatt ggtaatagat ttactatacc taaatttaag cttatgacag
    14941 ctgctaaata cactacattt atgatgcctg tacgagcagc atctgatacc ataccaataa
    15001 cacctactgg tcctgctaca gcattgccaa tccctcctgg tacacgtcct gtaatcattt
    15061 gacctagaaa tgttagcatc tgttttgtca tatctacagt agttttacat gcatttacta
    15121 ttgaccccaa aaagtctttt tctctttgtg gttgaatccc tatttcatac tttccattgt
    15181 tatttttagg tgttacattt acaactttat cacttccatc tctagttata ctaagtttta
    15241 attctccacc agaagaagta tttattatat ttgcaacttc atcccaagtc ttaacagaat
    15301 taccattaat tttatttatc ttatctccta cttgaagccc tacagcctgt gcaggagtat
    15361 ctggcattat ctttcctaat ttagtcgttg gagaacctat atacaaaaat actggaacta
    15421 aaagtattgc tgctaatatt atattgaata ttggtcctgc tacaatagta ctaaatcttt
    15481 gtaatataga cttgtttcca aaagaatttg gacttatttg ttcttcatct tcaccttcca
    15541 tgctgacata tccaccaatt ggaagtgctc ttatagaata ctctgtatct ttttttacac
    15601 tatatatctt aggccccata cctatagaaa actcatttac tttgatacca cttctttttg
    15661 caaatataaa atgtcctaac tcatgtatta atacaattat actaaatagt attaatgcgg
    15721 ctattatagt cataaattca ccttcctatt tttttatcag atttgcaaca taagctctac
    15781 tccatctatc tgtttctaag atttcttcta atgttggttc acttatgcta ctatgaactt
    15841 ctaaagtctt ctctatgtaa tatggtatgt catagaaacc aattttatcc tctaaaaatt
    15901 cacttactaa aacttcattt gctgaattta aaacagagga ataagttcct cccattttta
    15961 aacactcata tgccaatttt aaacatggaa aagtttctaa atctggttct tcaaaagtta
    16021 aagtactaaa ttttgaaaaa ttcattcttt caaagctact ttccattcta tctggatatg
    16081 taagagcata ctgtattggt agtctcatat ctggacatcc taattgagca attattgagc
    16141 tatctgtata ctgcaccatt gaatgaatta tgctttgtgg gtgaactact acatctatat
    16201 tttcttgttc tactccaaat aaccatctag cttctatcac ttcaagtcct ttattcataa
    16261 gcgttgaaga atcaatactt atttttcttc ccatactcca atttggatgc ttcaaagctt
    16321 catttttagt tatatttgca agttctccct ttttctttcc tctaaatgga ccaccagatg
    16381 ctgtaagtat tatcttttct atattttttt tattttctcc atttagacat tgaaatatag
    16441 cactatgttc actatcaaca ggaagaatat ttacattgta tttttctgct tctttcatta
    16501 caagttcacc agcagttacc aacgtttctt tattcgctaa tgctatgtct attcccttct
    16561 ttatagcaca aagagttggg actaatccta tcatacctac tacagcagtt aaaaggacat
    16621 ccacttcatc aagtgatgat attattttta atccttccat tcctgctaaa acttctattt
    16681 ctatattttt aggcaacatt tcttttaatt tcaatgcttt attttcctca aaaactgtta
    16741 catatttagg tttaaactct actatctgtt ctagcaataa ttcaatatta ctgttagctg
    16801 atattgcaac tatctcaaac ttatctctat tatctctaac tacatccaaa gtttgtttac
    16861 ctatagagcc tgtagaacct aaaattgata tctttttcat ataataatcc tttctaataa
    16921 taaatatacc ctgtcaaaac attataacac tactaaaaca attattttta ctattcatag
    16981 aaatttcata tattattcca cagattaaaa ttaatctatt attttttgta ttttgtggaa
    17041 tattataaaa tattctagta ttattaaatg ctctttttta tttccacaac atcttctatt
    17101 ttacagtcta gtgctaaaca tatcttctcc attgtatcca tagaaacaga tttatcattt
    17161 cctagtctgc ctaaaacaga aatacttata cctgatatat ttacaaattc cattttcttt
    17221 atttttctgt caattaacaa tttccataat ggagcgtatg aaatcatgtt atcacctcgc
    17281 aaaatatatt ctatcacatt ttatctattt tggaaaattt tcatctattt agataaataa
    17341 ttatt
//
LOCUS       DAMJWU010000076        17242 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMJWU010000076 DAMJWU010000000
VERSION     DAMJWU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.077"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(188..712)
                     /locus_tag="PC460_002642"
     CDS             complement(188..712)
                     /locus_tag="PC460_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2601522.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(777..1529)
                     /locus_tag="PC460_002643"
     CDS             complement(777..1529)
                     /locus_tag="PC460_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2601523.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1560..2858)
                     /locus_tag="PC460_002644"
     CDS             complement(1560..2858)
                     /locus_tag="PC460_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2601524.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2875..5139)
                     /locus_tag="PC460_002645"
     CDS             complement(2875..5139)
                     /locus_tag="PC460_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2601525.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5394..6212)
                     /locus_tag="PC460_002646"
     CDS             complement(5394..6212)
                     /locus_tag="PC460_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2601526.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6205..6903)
                     /gene="cbiQ"
                     /locus_tag="PC460_002647"
     CDS             complement(6205..6903)
                     /gene="cbiQ"
                     /locus_tag="PC460_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2601527.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6906..7184)
                     /locus_tag="PC460_002648"
     CDS             complement(6906..7184)
                     /locus_tag="PC460_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2601528.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7174..7926)
                     /locus_tag="PC460_002649"
     CDS             complement(7174..7926)
                     /locus_tag="PC460_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2601529.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8108..8300)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8432..10204)
                     /locus_tag="PC460_002650"
     CDS             complement(8432..10204)
                     /locus_tag="PC460_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2601530.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10218..11147)
                     /locus_tag="PC460_002651"
     CDS             complement(10218..11147)
                     /locus_tag="PC460_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2601531.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11376..11564
                     /locus_tag="PC460_002652"
     CDS             11376..11564
                     /locus_tag="PC460_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601532.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11557..12258
                     /locus_tag="PC460_002653"
     CDS             11557..12258
                     /locus_tag="PC460_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2601533.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12260..13183
                     /locus_tag="PC460_002654"
     CDS             12260..13183
                     /locus_tag="PC460_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2601534.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13340..14263
                     /locus_tag="PC460_002655"
     CDS             13340..14263
                     /locus_tag="PC460_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601535.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14256..14993
                     /locus_tag="PC460_002656"
     CDS             14256..14993
                     /locus_tag="PC460_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601536.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            15004..15777
                     /locus_tag="PC460_002657"
     CDS             15004..15777
                     /locus_tag="PC460_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601537.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(16318..16926)
                     /locus_tag="PC460_002658"
     CDS             complement(16318..16926)
                     /locus_tag="PC460_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2601538.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17104..17220)
                     /gene="rrf"
                     /locus_tag="PC460_002659"
     rRNA            complement(17104..17220)
                     /gene="rrf"
                     /locus_tag="PC460_002659"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5557 a         2994 c         2174 g         6517 t
ORIGIN      
        1 aaattttttt aataaaactt tattttttag ttaaaatcat acaatatata gatttccaca
       61 atacaaataa attaaaaagt gaaatgtatt tgtctaaaaa cataataaca tttcacttta
      121 tcctcatttt taagtaatat atatttttaa tttttattta taactaaatc tagtttttat
      181 ataaatttta gtttttttct acatctgagt aatctattcc aaaagtttct acagtaacag
      241 atttcataat ttgtggagtt tgtggcttat cagctgaatc tgttctaact gatgctattt
      301 catcaactgt atccatacct tctatcactc taccaaaacc tgcatactga ccatctaagt
      361 gtggtgagtt tttatgcatt atgaaaaatt gagaaccagc tgaattaggt gccatagttc
      421 tagccattga tatgactcct ctttcatgtt ttaaattgtt agtaaatcca ttaccactaa
      481 attcaccttt tattgagtga cctggaccac ccattcctgt accatttggg catcctcctt
      541 gtatcataaa tcctggtatt actctatgaa atattattcc attgtaatat ccttcattta
      601 ctaatgatac aaagttttta actgtatttg gagctatatt tggatataac tcaattttga
      661 tttcttttcc attttccatt tctatagtta ctataggatt tttattttcc ataatttatt
      721 accttccttt tcattagtta aattacataa ttacatttat tatattatac tctaaattaa
      781 agtctatcaa gtatattttt tcctatttca tatccttctt gataaatagt tttacaaaac
      841 tcatcatcaa agttaagcgt gtctttagga gcaaattgag tattaggtct aactactatt
      901 attttatctt catcatatac ttttttaata tcttctggca actcataatt atattttgtg
      961 gatattaaaa taattttatc cacattatcc ccaattaatg gttcaattaa tgcactccta
     1021 attaaacctc catctaattt gaaaccatca taaattcctt cttgcacatc tttcacaaat
     1081 tgttctttaa atccaagagt tgcgtttgga tttgatggta acaacccact gtatgaaatg
     1141 ctgataatgg cttcattacg aggcattttt gatacatcaa caatcttttc tgatactact
     1201 ttattatcaa cttcaacata attaacataa aagttcattt cttctatatt actatcatct
     1261 agttccctta ggttgtctat agcaggagaa ttatccacag tattatttac tatttttaca
     1321 tttccattct ggatatcttt tatatttgtc cacattttct ccaagttatc aacattccct
     1381 gtgtacaaat aatatccatt tattgctcct attgatgtcc ctgaaatcga ataaaattta
     1441 tttattcctc catcatacaa ggctttaact actcctgctt gataagcccc ttttgctcct
     1501 ccaccttcta aacataatcc taatttcata gtctcatctc caaattgttt atcttatatt
     1561 tatatttcaa aaaacttact aaatgtttta tatatactat agtggtcctc aacatttcct
     1621 aattctctta tagaatgcat tgctaaaatt ggacttccca catccacaga tggtatatct
     1681 atatgagtag aagatattgg acctatagta cttcctcctc tttcatctga cctattaaca
     1741 aattcttgat actcaacatt agcttctcta catatatttt tataaatagc tatagaatat
     1801 ccatcactag tatacgcctg actagcattt atctttataa ccggaccttt tcccattatt
     1861 ggtttattag ttgggtcatg cttttctgga atgtttggat gaatagcatg agctaaatct
     1921 gatgatatca taaaagatga ataaatagac tcaaagaatc cctctctatc cttacccatt
     1981 gatatacaaa ttctctccaa tatatttaac aacatgtttg aatctgcacc ttgtttagta
     2041 gagctaccaa cttcttcatt gtcaaataca gccattacat ttacaccatg ttgaccttta
     2101 gtattaacaa gagcattcaa actagcatgt accattgcaa gattatcttg tctcccaata
     2161 gatataaatt catcattagc ccctattaaa cttccttttt caaattcata taaaaataaa
     2221 tcaaaatcta taatatcgtc catatttaca tctaagttag atgctatttg ctttataaga
     2281 aaattatctt tttctaatgt atcatttaac atcccaacta aaggtaacat atctttttgc
     2341 ttattatatg aatgaccatc atttatactt ctatttaaat gtattgctag atttggtatt
     2401 atacaaattg gctttttaaa gtctataagc ctttcatttg gttttaatat actatcacct
     2461 tttaaaacta ctctaccagc tatcccaaga ggtctatcca accatgtact taatattgct
     2521 cctccatagc attctgtatt taacttcaaa taagattttt cagcttctat ttctgcattt
     2581 ggtttaatac taaatgtagg agaatctgaa tgagagccta ttattctaaa tccatctctt
     2641 tctatatctt ctgaatttac tacaaaagct actatagctg ataaattctt agtaacataa
     2701 tactttccac caactcttag attccatctt tgattcaaaa ctaattcttc aaatccattt
     2761 ttatctaaga tttccttagt actcttgact gcatgaaaag acgacggact atcatatatg
     2821 aagtctatta agtttttagc aaagtttata ctatccattc ctcatttacc tccattataa
     2881 aatatttagt gttgtatatt ctttatttat ttttttaatt ccctgttggt caaaagctat
     2941 agtaacatct gtaccaatta tagaaaccac tgtcccaacc ccaaatttag gatgatgtac
     3001 cttagaacca agttttatat catctggatt agtttcttta ttagaatctt ttattgtagc
     3061 attaacttta tctgcaactt tagatttgct tatagtactc atatactttt tagtatattt
     3121 atctaacata ttataacttg ctttagaata gcttagttct ttttttactt tgtacaatct
     3181 ttctatacat tcttctggca gttcttccat aaatcttgaa gctattgcaa catttgtttt
     3241 accatataat gttctcttct ctgtcaatga catatatagc tcttctttag ctctagttat
     3301 accaacataa caaagtcttc tctcttcttc tatttgtgaa tcactcatag attttatagc
     3361 tcttgatata ggaaataacc cttcttccat acctgttaaa aacacaacag ggaactctaa
     3421 acctttagat gtatgaattg tcataagcga aaccctatca ttgtcttctt catcatttga
     3481 ttcagatgta agtgctatac ttgttaggaa tgtctctaag tctttatttt cataagtatc
     3541 atcattacta ctttcaaact ccaaagctat agaaataaat tctctcaaat tatcaattct
     3601 gtcttgagct tcttcacctt tgcctgttaa atctttttca tttttatttc ttatctctac
     3661 aagctcgtcc atatatcctg tagtgtccaa gactttttct ataagtttgc taacaggata
     3721 aacttctttt atagttctaa gagtccctat aatatcaaca aatccactta tacttgccct
     3781 tgccttagta gatatatctg aatttgtttc tatatcaatt aatactgagt atatactttc
     3841 ctgttttaag ttagctctat cttcaatttt ttctatggtt ctaagcccta tacctcttct
     3901 aggaacattt ataatccttt ttatagatat atcatcttgt ggattttgaa taactcttaa
     3961 atacgccaac aaatccttta tttcttttct ctcatagaat tttgtaccac catatatatt
     4021 gtatggaatt tgacttctat ttaaagcgtc ctctacaggt cttgcctgtg catttgctct
     4081 ataaagtact gcaaaatcct tataaggtct attttctttt ctagctattt gtgcaattga
     4141 atcagcaatg aaatcagcct cttctatttc actaccagtt aattgtatct ttataagttc
     4201 tccttctttt ttttcactcc aaagcttttt tctttttctt tctatattat tagatataac
     4261 tttattagct gcatctaata tcacttgtgt agacctataa ttttgttcca actttacaac
     4321 atgtacattg tcataatctt tctcaaattc aagtatgttt cttatatctg cacctctcca
     4381 tccataaata ctttggtcat catctccaac aacacaaata ttttgatgtt ctcttgctaa
     4441 taattttatc aattcatatt gagctttact tgtatcttgg tactcatcta ccattatgta
     4501 tctaaatcta cttctgtaat atgctaacac ttcatcatta gccttgaaaa gttctacagt
     4561 tttaagtatt aaatcatcaa agtcaagtgc actatttctt tttaaacggt cttgatatag
     4621 cgcataaata tctgcaatct taaccattct attatctgcc atattgatat ctttaaactg
     4681 ctttggagta taaagtttat cttttgctcc tgaaattgca cttataactg cttttggttc
     4741 aaacacttta tcacttaaat ttaattcttt tagacagtct ttaaccaatg taacttggtc
     4801 tgctgaatca taaattacaa aacttctatt gtaaccaatc ttatttatat cttttcttag
     4861 tatacgaaca cagcaagaat ggaatgtact tatccacata tccttagttt caggacctat
     4921 attttgttca actctctctc tcatttcatt tgcagctttg tttgtaaatg ttatagctaa
     4981 tatatttggt gcttgtacac ctttatcttc tataagatat gctattcttg ttgttaatac
     5041 cttagtttta cctgaaccag caccagccag tataagtact ggaccttcag tcttttctac
     5101 tgcttctctt tgagcaggat ttaatgtatc taaattcata tttctcctcc tgttaacagc
     5161 cataaatata ttttaatcta aaatatgaat tacttcaaat tataattatg tattatatta
     5221 atagattatt tccaatatat tacaattaaa taacattttt ccatataatt tatttcattg
     5281 tttaataaaa aaaataacct tatgatttgg ggaatcataa ggcaccttta aggggtctag
     5341 gttaagggtt attatatata aatctatttt tttaaaatag gtttagggct taattattta
     5401 tttgtattaa gttctctttc cttccaatac ttatacaaat cttcttcttt cttaaataag
     5461 ggtaaattca tatttctatg tacttttact aaccatgggg attctaggcc agcttgtata
     5521 atattatttt catttataaa cacattttct gcttttcctt catcaatcaa aatcccctta
     5581 tccaatacat atatgtaatc acatatttca tacattaaat tcatatcatg actggatatt
     5641 actattttta tattattttt atttaatccc tttattatat ctacaataga cctagtactt
     5701 actgggtcta aaccagctgt aggttcatca agtaatacaa tttcattttc cattgctata
     5761 actgatgcta ttgccactct ttttttctgt ccatagctaa gaaaatgtac tggcctgtct
     5821 ataaaatcta ttccattaac agctactaga gctttgttta ttctctcttt aattgttttt
     5881 tcatccatac caatgtttct cattgcaaag gctatatcat catatacttt tgaataaaaa
     5941 atttgttttt ctgggtcttg gaacacaata cctacatttt ttctaaggtt gtataaactt
     6001 ctcttatcat attttaactt ttcttcttta aaaagaattt ctccagatgt aggctttaat
     6061 atacccatta aattcataaa taatgttgat tttcctgaac catttgaacc tattatacct
     6121 ataacatttc cctttgaaaa atccatatta atgtttaata gtgcattggt atttttttca
     6181 tattgatacg ttaaattatt tattttaaac atcattatcc cctactatgt gaaatttacc
     6241 atcatacaat tttatgtcta atgatataca catatcatca taccttgtca taagtctttt
     6301 gtacagcata cttccaagaa tacctaagga tttatatgaa gtttttaaat ttatatatcc
     6361 aaatcttagc tcttgtgact tgtatatttc tgaaaattct tctaaaaata tgaatataaa
     6421 cctatatatt aacattgata actcgacaaa agtgtcaggt aaatgaagtt ttttaaaaaa
     6481 gaatattaat tgattaaaag gtgttgtaag tatacaaaaa tacacacaag tcaaacaaga
     6541 catcgctcta aataacacat gaatagacat gtcaatagac gcctttgata ttcctacatt
     6601 aaaactaaaa aattcaaaac tatataataa atctgcttta ttaaaagata tatttacaag
     6661 agttattcct atactaagaa ataaaaagta cattggtatt ctaagtaatt ttaaataatt
     6721 ttttaaatct attccagcca cacaaacaac caaaatactc attaaaatca taattccact
     6781 taaaataaat atattttgta ttaccataga agcaatcaga aaaattactc ctatggctac
     6841 ttttttgttt ggatttactt tactcaatct atttgtatat gcatatttat caattataag
     6901 catgattaac ttctctttcc tttttgaaga cctaatatgt atcctattac accagcccct
     6961 aaagctgctt gtgcagagaa taataaactt tctatttctc cacttggtgg ctcatatgga
     7021 ctgctaaacc atggtttgta atttggattt atcttagtaa tttcactttc agcttgacca
     7081 tctgctcctc catattcagc acctgagttg acaagtaatg ggaatataat aagagctact
     7141 actaataaaa gtaataatat gttagttttt gttttagcac tcattagctt taactccttt
     7201 atcattattt tctagtatgc ttttttctcc gttttctgta attaaattat atacaattac
     7261 agtaagtata ccttctgcta ttgctattgg tatttgagtc ataaagaaaa ttcctaaaaa
     7321 tttaattgct gaagccatta ccccacctga tgggtctggg aaagccagtg ctaattgtat
     7381 tgaagttatt gtatatgtgg ctaagtctcc tattgctgct gcaagaaata ctggaatagc
     7441 attatttcca tcttttttct ttaaagcctt aaatattagg aatgatacga ttggccctat
     7501 tatcgccatt gaaaaagcat tagctcctaa agtagttatt cctccatgtg ctaataacaa
     7561 tgcttggaat attaaaacta tagttccaag aacaaacatt acgcttggtc caaacattat
     7621 tgctccaaga cctacaccag taggatgtga acaacttcct gttactgaag gtatttttag
     7681 tgctgataat acaaacacaa atgctccaca taaagctaat aatactttct ttttagggtc
     7741 ttgctttact attttaccta ttgattttaa tccgaataag aaaaatggta taaatataac
     7801 tccccatgct atagaccatt ttactggtaa gtaaccttcc attatatgca ttgcatttga
     7861 tgtaactgga gttaaaatga ttaacataag tgcagctata acccctaatt taatattctg
     7921 tttcatctta gtatccctct ctccctttct aataattaaa acataataat acatgtctga
     7981 tttatttaat aaaataaaaa agcctaactc gatggttagg cttctaattt tccacaatct
     8041 aaatacaaac acataaaaca aacatatctg tataattatt ataagaaact ttctccatcc
     8101 ccgaagaaat tactttaata ttttaggcag gtctcctgac ttagcttcat cctactcctc
     8161 ttccttccca tgagatataa aaaatatcat catagtggct aacaaaagtt tcgtctgcat
     8221 cacagtagcg ggggctgtaa aggaatttaa cctttttccc tattaatctt gcttaaagaa
     8281 cctataatat tatatattta atttttaaat tcttcttaaa ttattttcta tataaatcat
     8341 tgtatacctt ttctgtgaaa atttctacat tttttataaa ttttattcat ttttatattt
     8401 ttatgtatat aaattctttt ttgtaagttt attaatttct aattatcttc tttaaataca
     8461 ttgaaactac atcagaaaat ctagttatat ctcttgaata tataaaatca tttccatata
     8521 ttaatctttc agcttcaagt tctgattctt ttcctgtaaa tactcctaat actaatatat
     8581 tttgctttct agcttttctc acttcattgg ctgtatctct caaaccaact atacccctat
     8641 aagccttttc tcctcttaat gttctctcac tactttttcc tatctttaca tcatttggtc
     8701 ttccatcact gagaactatc aggattttat tttcttcatc tctatttatt agaccactac
     8761 atatagattt tattgccaaa ccatctctat tattaccagc acaaaaatat tcaaatatat
     8821 tttcactatt ttttatacta tcatcataat ctttaaatct ttttaatatt gtatagtcta
     8881 aaaaactaga aaatcccatc actctatttg gtatgccaac ttctgttaga gcatttgcaa
     8941 ttatgtaagc ttgaattgcc acatttccct gatttctact ttgagagcca cttgcatcca
     9001 ataatatatc aataacgtat ttcccttttt cattttctat atctttataa aatattttat
     9061 tattattact tctaccaact ctccaagctc tattggctaa tatagtacca caatcagaat
     9121 atactctact tttagcattt tcttctatta aaattcttga tagactttct tttaatctct
     9181 ttatttgctg tctatacatt ctagcatgtt ctctaaattt tcctaaatta ttttcttttt
     9241 gtcttgttac atattttagt tgaaatacat tattacactc acttctaaga actccatctg
     9301 taaagtgtat tctacaacct tcatgaacat ttctacaatt ttgtgtctca atttttttaa
     9361 tttcaccttt actcaaataa gtttttccat aataatgttc tatttttgaa tatatcttat
     9421 ttgcagtgtc ttcatcaaca tatataactc ggtctgctga attgctacat ttagaatcat
     9481 caaaatcccc tacaccttct acaagcatgg atgaagatat tgaatctata tcactttcaa
     9541 taatattttc ttcttcatca ctataaagtt cttcatacat gaaatcagca aatgtatcaa
     9601 aatttacttc tacactttct acattatttt catcaataag actattttct tctccattta
     9661 atatgtattc aaaataagtt aaatatataa catctaatgc ttttaaaatc tccataatat
     9721 cttgattggt atctatattt cttatatcat taactattct tctagtcata ctatctaatg
     9781 ctgggtgctt gtccatacta taaagtacaa atgtgtattt aattttatcc ataatattat
     9841 cattgtgtat tttggtaaaa cgtgaaacaa tatctttata agcatttctt cttatgtctt
     9901 ctatccctgg cctatcttta atgacctttt cttcaactat tgtatttaat ataacttgaa
     9961 ttagacccag tataatatct ttgttgtatc cttttcttat tctatttgta agatattttt
    10021 taactatatc ccaatctaca tactttcttc tagcacctgc aattattgca aaatatagac
    10081 ctatttcttt tgaagagtaa tctttttctg atatatctat actttcattg taatcaccag
    10141 ctattgtcca tgataagtta ttagctctat tttcaaattc ataatcatca tatatccatt
    10201 tcataatctc acctatatta aatatgactt attggaaaca catctatact ctcccattta
    10261 ttaggtattc tagttttaat tacatctcca accatttctt tctcatatac atcaaaagtc
    10321 tttcctgtaa gtcccatatt aattgcaaga gttggtttta atcctcttct tatagtctta
    10381 agagatgcca taagaccacg taaatcaata gctttactag atatctctcc attttgagac
    10441 ttcaattgta aatctaaaaa tattcctgca aagtgtatta gtttttcttc atcagcatct
    10501 gaaaattcat tttttaaaat catcattaac ttatcttctt ctataggagg aatatctatt
    10561 accataaatc tagataccag tgcttcatta agttcttttg ttcctgcata ttcataattc
    10621 atagtcccaa taaatcttgt tgctggatgt agattgactc tctcataccc aggtacatca
    10681 attattcttc tatagtctaa agctgaatgt accacaacta tagcatcatt ttttgccata
    10741 ttaatttcat caaacacccc aaaacctcca ttaatagcac actcataaac tgagcctctt
    10801 ctaagtttaa cttcattgtc tataaatgta tctgtaccta taagtgttga actgtctgta
    10861 tttatatgaa atgatgtatt ccactgaggt ctattaaata tttcccctag attatcagca
    10921 agtaagtttt ttcccgttgc ctttggtccc gataataata tattttcttc ttctaaaata
    10981 gctgctatac acatagacaa gatatcttta ccataaaaat aagttttaga ctttgttact
    11041 ctgtattcta aatctttttc taatccataa taatttctaa aatacagtac atcttcaata
    11101 agtttttcat ctaccccttg cacccttaga ttatctatta tatccatatt tttacccctt
    11161 ttatataaat agtatttgtt tcatttctta tcaccatttt agcataaaag gatataagct
    11221 aaaattgatt ttttaatctc ccccatttta agtaaaacca tgcaccattt tatcactaca
    11281 aaattaagag tactacttac tgatatttct tgattttatt cgtctatacg tatataatat
    11341 atactgataa attatgagga aaggatgatt gaaatatgga atatatgtct tgcccagaag
    11401 tagcaaaaaa atggggtatt tctgaaagaa gagtacaaaa actatgtgaa gaaagtcgca
    11461 taccaggagt ttcaaaactc gggtatatgt ggctgatacc aaagaacgca gaaaagcctg
    11521 ttgatggtag aacaaaaaga agaaaggaag tacaatatga atagcgtttt gattatagat
    11581 gatgacaaag aactttgtgc tcttatgaaa aaatgtgttg agcaagaaaa tttatctgct
    11641 attactgcac atggtggtgc agaaggtcta catttagtcg ataaaaatag acacacactc
    11701 tctcttgtaa ttttggacat aatgatgcca gatatagacg gttttcaagt tctgaaaaaa
    11761 attagagaaa caagtaatat tccagtaatt atgcttactg ctaaaaatga cgaggatgat
    11821 aaagtttccg gactacgttt aggtgctgac gattacctaa caaaaccgtt cagcataaac
    11881 gaacttatgg ctcgtgtcaa ttctcttatt cgtcggtata ctatgctaaa ccctgctttt
    11941 ataacagaca cagatttcat aataataaat ggcatgatta ttgacaagac aaaccgaatt
    12001 gtttctgtca ataatattcc tgttcaactt acaagcaaag aatttgattt acttgctttt
    12061 ctggcttcta ataagggacg ggtgtttacc aaaaaacaga tttatacaca agtatgggaa
    12121 gaagaatatg cttttgacga tagtaacatc atgtctttca tcagtaaatt gagaaaaaag
    12181 attgaaccag accccaacca tccattttat attttgacag ttcgtggtgt cggttatcgt
    12241 tttaataagg aggcttaaca tgaatacaag tgtctatctg cttttgatat tggctcttag
    12301 cctgattatt atagcgtacc ttgtttctgt cctgctacgt gtccgaatac aacttacact
    12361 tataaaagat gctttagagg atataaaaaa tggaaacctt aatcgacgta ttttaacaga
    12421 tgaaaacgac attacaaaac aaatttgcta tgatattaac gaaatagcaa tcaatagcca
    12481 atttcaactt attcagcaaa aacaatcaga acaagcctac aaacagctaa tgacaagtct
    12541 ctcccatgat gtaaaaactc cacttgcttc tttggtcggt tatttggaag cagtagaaag
    12601 taaaattgtt gtaggagagg aaaaagacga gtatatccat gttgcgtcta ataaggctca
    12661 ttacttaaag aattttgtag aaaatctatt tgaatgggta aaattggatt ctaaggaaca
    12721 agtttttcat tttgatattt ttgacttgaa tgaattatcc cgtaatatta tatctgattg
    12781 gatacctgtt ttagaaagca gtcattttga atatgaattt gatataccag aaatagagta
    12841 cttcttacgt atagacgcaa atgcatatac ccgtatcatt aataacctat tacaaaatat
    12901 tattacccat agtagtggaa acaaaatgac attgagaatt tttgaaaaca aagagcaagc
    12961 tcaaattata ataacagata atgggaaagg tatatcttca gacaatctcc cacatatttt
    13021 tgaaaggttg taccaatgcg accattcacg agcttcaaag ggaaatggtt taggcttggc
    13081 gattgcaaag gaacttatta acgctcataa gggaaatatt accgccaaca gcactcccgg
    13141 tatgggaaca gaatttacaa tattgcttcc taaagccctg taaaagtacg aggctttctt
    13201 actctaaaac aagataaaag caagattatg gcaaggtttt ggcaaggttc atgttttata
    13261 attctaattg tagttgattt tgacgttgta tcaactatct tttgtagaaa caaatttatt
    13321 aaaagtgagg aaattatata tgaatgattt tgtgattgaa acaaagcaac ttacaaaaat
    13381 ttatgggaat caaacagtag ttgacaaagt caatctccat gtaaaaaaag gccgaatata
    13441 tggtctgtta ggacgcaacg gagctgggaa aaccacaatt atgaaaatga ttttaggatt
    13501 gacttcaatc acgtatggcg aggtagatgt atttggagag aatattaagg gacgtgaaaa
    13561 acaagtatat ccccgtatcg gtgcaattat cgaaacgcca ggattttatc ctaatctgac
    13621 aggaacggag aacttggaga tttttgcaaa actgcgtggt acagccgccc ctaattctgt
    13681 aaaaagagca ttggaaattg taggcttacc ttataaaaat aaaaagctgt ttagtaaata
    13741 ttcacttggc atgaaacagc gacttggtat tgccaacgct atcctgcatg acccagaact
    13801 gttagtttta gatgagccaa ccaatggtct tgaccctatt ggtattgcgg agatgagaaa
    13861 gtttattagg gacttgagcg ttgagcatgg aaagacaatt cttatttcca gtcatattct
    13921 ttcagaaatc tcattgttgg ctgatgatat tggaattatc gaccacggtg ttcttttgga
    13981 agaaagtagt atgagtgaac tagaaaagaa aaacagtaaa tatatcctgc tacaagtttc
    14041 agatattccg aagacatcac ttattttaga gcgtcaattt aacacgaagg attattccgt
    14101 tcaagatgac tacacgttac acctttataa cactaatctg gatatgggtg aaatcaataa
    14161 agcacttgta atgcagaatg taactgttat tagttctcaa ctttgtaatg atactttaga
    14221 ggactacttt aaaaaaatca caggaggaga gggcattgct taaacttatt caaacagaat
    14281 ttcttaaact gcgtcgtagg aaacttattt ggctgatgtt gttgtctgct ctcattatgc
    14341 cgtttttcgc actactatac ttcaattatt ttggtaaaac aggtgttgag ccaataaagt
    14401 tttataaatg gtctgcattt ggttatacac tttggattat tttgcctgtt gttttgggta
    14461 tattttgcac catgcttata cacgaagaaa accagtatga tatgctcaaa cagctttgga
    14521 ttgtacctat tagtaaaatg ggatactttt ttagtaaatt tttcgttgtt ttgatttact
    14581 ctatttgttt tatgctgatt acagccgttg cttctgtgtt atttagtgta ctttctggat
    14641 atgtcgtatt tagctgggca agcattttat atttgttgaa aaaatgttta gaaattggtg
    14701 taatcactgc ttttgtaatg ttgccgattt tggcaatcgc tacatctcaa aaaggataca
    14761 ttctcccaat ttgtatcaca ttggtatatg ccttttccgg ttttgttata atgacaataa
    14821 atatgtattt acacccatta tccagtatgg gagttattat catgagaaat aaagacattc
    14881 caggtcttat attttcacag gaaattaatg tcccattagc atttctttgt atttgtattt
    14941 ggagtattgt atcagtattg tttgcaaata ttgcactagg aagaagaaag tgaggtgtag
    15001 ataatgtgga aattactttg ggctgaatgt caaaaaattc gtcgttcaaa aattgtttgg
    15061 attacagttt ttgcatccat tatgatagca gtcatagttt ttgcaggggg gcaggatgta
    15121 tataatgacc cagatttaca ttatggctta aagagcgttt atgacggttc acggtatata
    15181 gacaatgcag gttggtatat ggacgcagta caaccttggt caactttttt tgttcttcct
    15241 gctgtcattg ccctattagg aagctatatt atttgtcgtg aagaagagga ggacactatt
    15301 aaatccctgc gtcttattcc cataaatgaa acaaatttaa ccatagcaaa aatgattatt
    15361 acttttatat tcagtatact tctttatttg ttactcttta ctataacatt tttgactgaa
    15421 tccgttttac atttttccga tttatcgaca aaattggttt taagttgcat gaaagagtat
    15481 tttcttgatg gcataggtgt attctttgca atttctccca ttattgcctt ggtatcgcgc
    15541 atgaaaaaag gatattggct tgcattagta ttcacagaag tttattctat tgctggacta
    15601 tttgcgggta tgtcaaatgt cttacaaacc ttttttccta ttgcggcaat atttaatatt
    15661 tctggatact atataactac tggagaaaaa attatgggga gtatcatgag tttattactt
    15721 tgtgcctgcc tttctgcatt tatacttaaa gggctaaaac acaatgaaaa aaattgaaat
    15781 aggtatatca aaatgaaaaa gataaaaaaa ctgattattc taattgtatt cttgtttctt
    15841 tttggcatgt tacactgtta tactgaatgg aaattcaaca ataacccacc taaaggggta
    15901 atagaaagaa taaatgagtg gactgataag attggactgg attagtaact tatgttaatt
    15961 atagtgaatg taatattagc aagttattgt ttcgtatttt aatactataa ctttaaatat
    16021 ttggttttaa tcttccaaac gaatataaaa aaactgtcac agaggcaaca tatttttgaa
    16081 tatttataaa gtggtagttt tgattttcaa agctaccgcc ttttcttcta tataagcaaa
    16141 cttatcctca acaaagtgta aaacatggtg cattttaaga aaaaagtacc acagacacaa
    16201 gcaataaaag aaacgccgag aacctacaaa aacgtagtgc ttatgcagat ttacgacgcc
    16261 ttctattttc tcaaccgtct taatgacctt gtacaaaaga aatttttcta atatttttta
    16321 taatcttatc ttgtgatgtt ttttatgtat aaatgactta ataacaaatc caataaaaat
    16381 tatggatatt ggtataatta gaacgaggaa tttataatca ttataatgtg acattattct
    16441 tacccaactc tcagaaaaaa tatatcctat actgattaat gaagtattcc atatggttat
    16501 tcccacgata gatgatatta caaaagttat aatattttga cctgctatac ctgctataaa
    16561 agatatgtat gttctacata aagggattaa tctacataca caaacagaaa tagaagagta
    16621 tttattaaaa taatcttgag atgcaaatat tcctttcttt gcctttggac atatctctat
    16681 tattttattt atgatagatt tacctcccca atttcccact gtataacaaa atatcgaacc
    16741 aagtatacct gcaattatac ttaaaattaa aattgtcaga aatccaaaat gatttcttgc
    16801 agcaattgct ccacataaag gtaaaacaac ttcgctaggt aatggaaaac aagcatactc
    16861 aatcataatt aatacaaata cagagattaa tccatattgt aaaataaaac tattgattac
    16921 agacacttac ttatctatct cctttcttat ttaaaggttt taagggtaca tgttctaagg
    16981 attctcaccc tatatttaaa gaatcaatct ctttaaacta ttatatgtat ttacacaaag
    17041 catttataac atactttaaa aatagtacct taaaataaga aacttaataa ataaaaaaag
    17101 attacgtggc tacgtcctac tctcccaggc agtttcccac caagtaccat cagcgctaga
    17161 gagcttaact tctgtgttcg gaatgggaac aggtgtatcc tctctgctat tgtaaccaca
    17221 taatctttat gcttaatatt tc
//
LOCUS       DAMJWU010000077        17106 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMJWU010000077 DAMJWU010000000
VERSION     DAMJWU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.079"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(199..2265)
                     /locus_tag="PC460_002660"
     CDS             complement(199..2265)
                     /locus_tag="PC460_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2601539.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2342..5371)
                     /locus_tag="PC460_002661"
     CDS             complement(2342..5371)
                     /locus_tag="PC460_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2601540.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5424..6884)
                     /locus_tag="PC460_002662"
     CDS             complement(5424..6884)
                     /locus_tag="PC460_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2601541.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(6976..7350)
                     /locus_tag="PC460_002663"
     CDS             complement(6976..7350)
                     /locus_tag="PC460_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601542.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7526..8266)
                     /locus_tag="PC460_002664"
     CDS             complement(7526..8266)
                     /locus_tag="PC460_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2601543.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8287..9075)
                     /locus_tag="PC460_002665"
     CDS             complement(8287..9075)
                     /locus_tag="PC460_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF2601544.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLTFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(9163..9253)
                     /locus_tag="PC460_002666"
     tRNA            complement(9163..9253)
                     /locus_tag="PC460_002666"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9217..9219),aa:Ser,seq:gct)
     gene            9512..10276
                     /locus_tag="PC460_002667"
     CDS             9512..10276
                     /locus_tag="PC460_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2601545.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(10557..11672)
                     /locus_tag="PC460_002668"
     CDS             complement(10557..11672)
                     /locus_tag="PC460_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2601546.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(11713..13239)
                     /gene="glpK"
                     /locus_tag="PC460_002669"
     CDS             complement(11713..13239)
                     /gene="glpK"
                     /locus_tag="PC460_002669"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2601547.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            13532..14629
                     /locus_tag="PC460_002670"
     CDS             13532..14629
                     /locus_tag="PC460_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2601548.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(15082..15594)
                     /locus_tag="PC460_002671"
     CDS             complement(15082..15594)
                     /locus_tag="PC460_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601549.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KMDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            16147..17028
                     /locus_tag="PC460_002672"
     CDS             16147..17028
                     /locus_tag="PC460_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF2601550.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSIKDVEKFINFFSAIPAKDTDDTIKISEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
BASE COUNT         5695 a         2915 c         1816 g         6680 t
ORIGIN      
        1 tatctttcac attgttgctt ttctttaata atactaaata atattttcca cattgttgct
       61 ttttctaaac aactataaat aatatattaa acccttattt ctttttctaa taagttaaat
      121 aatacatttt acacatatct atttgtcttg cacataataa ataatacatt atatttctat
      181 ctaaatttag attctaaatt aaatagtata aattatattc ttcaataaac aagttttcaa
      241 atccaagatt cgctctctat ctttaaactt tattgtaaac ataattccat cttttttaat
      301 tatttctcct tcatgaaatt ttttatggta taccttatcc cctacaccaa tactgtcaat
      361 ttcttttttc gttggagatt ttatatcatt aataaataat gatttatcaa ctttcttacc
      421 atacttattt aaagtagagc ttatacataa cttttcttca gctcttgtaa tagcaacata
      481 cataagtctt ctttcttctt ctaattgttc ttcttttttc tcttccttac aaatatcata
      541 tgatttttca tgaggtattg ttccttcatt tacacctata atataaacat ttctaaattc
      601 taatcctttt gcactatgca tagttgtaaa tatgacaccc tcagtctgct tattcttgtt
      661 attttccgtt aactcagtct ttactctatc aatatgctct aaaaactctg taacagtatt
      721 aaaattagtt gctgaactct caagttcatt taatatctct acaagtccat tagtctttat
      781 ctttctgttg ctacaataat ctaaaacata tctatcataa tcaagactag ttctaatata
      841 agatattgca tttttaggat ttaaagtatt taaataactt aaatctattt ccaaatcatt
      901 tattgtttta acttgtttag gatgaagatt acacttattt attaatgctg ttataaagtc
      961 tttttcatcc ttaaccatat ttatactgtc tttggatata tatctaaaag gcttgtttat
     1021 aattctcaac caatctttat ttgattttgg attaatacct attcttaaat aggctagtat
     1081 atcttgactt gcccaatggt catatattgt aattactgag tcttttacta caaatggaat
     1141 cctcatatcc ataaatacat ctactaatgc tcttgactgt atattagttc tataaattac
     1201 tgcaaaatct gaatactcta cataatcttt ttgtatttcg tcaattattt ccctggcaat
     1261 atataaggct tcttcctctg aatcatgtgg agaaatatag gtaacacttc ctccatcccc
     1321 ctgactacac ttaatgattt tttcatatct atttttattt ttgtcgataa gtctatttgc
     1381 agtatgcact atctcacttt tagacctgta atttatatct aatattattt ttttagtatt
     1441 attaaagtat tgttcaaatt caagcaaaaa atctggtcta gcacctctaa atccatatat
     1501 actttggtct tcatctccaa ctgcaaatat attgtttaat gggctacaaa ttaactttaa
     1561 aacttcaaac tgtactttat ttatatcttg aaattcatct atcaatatat atttatacac
     1621 atttctaacc atttctagtg cagacttatt gttcaaaagt aaataataag ttcttataag
     1681 catatcatca aaatctattt tattaacttt agatttttgt tcttcgtata agttgtatac
     1741 ttttaaaaac tcatcttttg ttaatacttc tgaattaaat tcatttttat ccataagttc
     1801 attttttaca taggaaattt catttataac ttgtcctata ttctcatcat catcagcatt
     1861 ctctatattt aaacttttta ggattgcctt gattgtcatt cgctttgact tttcatcaaa
     1921 tatactgtct aagttgtatc tttcaaagta tcttagaatt ttaaaaaata cagagtgaaa
     1981 agtaccatag gttaccttat taagtcttat gtcatcactt aaattcaaag ctctattttt
     2041 catttctatt gaagatgcct tggtaaaact tattgccaag attcttgttg gtgctatgtt
     2101 tttgtttaca accatattgg ctattctata tgtaattact ctggtcttac ctgaaccagg
     2161 ccctgcaagt atcatgcaag gcccatctat atgctcaact gcttctaatt gattttcatt
     2221 taactttctt atatctatat ttgttgtgat tattagctcc accaccttga tttataatta
     2281 tattattttc tttttaatta taaactaaaa gttcataaaa ataaatttaa tttttcctct
     2341 attaaaccac atcattccta ttttcatgac atatcttgag ttctttttta attttgtcgc
     2401 ttatatcttc aggtataaat gatggtttac taatatagta gccttgcata taatctactc
     2461 caagattaat tagtgtttcc atttcacctt ttgtttctac accttctgct atcactttaa
     2521 tatttctttc gttagaatat gagataagat tttttaatat tttctgtctg ttttcatctt
     2581 tatcaatacc tcttacaata gacatatcaa tctttacata gtctggcatt atatataata
     2641 gtacagcatc tccgttgtaa cctgtcccaa aatcatctaa agcaatttct atattccaat
     2701 ggtctatata tgctttcttt ttttcaataa aatcttcatt aatcttatca ttctctgtta
     2761 tttctagtac tactctattt aaatattcac catataaatc ttcaatttcc tcaaaatcta
     2821 cttttgacaa attattatta ggaatagaat ttaagaacaa tttgcaatcg ccaaattttt
     2881 ctttgtattt tttaaatgat ttaagtgctt taaaccatgt aatacgctca atttgatata
     2941 atttagactg tgaacgagct aatctaatga catctattgg tgaactaaaa gtttttattt
     3001 taggtctcat aagtgcttca tatgcaaata ctttaccttt acaatctaca ataggctgaa
     3061 atgcatactc aactaattga ccatcaatta acttgtttaa ttcttctcta ttactcaata
     3121 agaatgaatc cttattatat atattaatat caaattcact aacttcaccc ttgattgtat
     3181 ttttaatttg atacatagca aaatctgcgt atctcattaa ttcatcatag ttggttgagt
     3241 catcagggta ccaggaaatt cctgctgatg cccttatttt aaattctgtt ccatcatgaa
     3301 gttttaataa ggtattattc atctgtttct taacagaatt aataatatct cttatactat
     3361 ctttgctttc atatccatat ataaatacat aaaactcatc acctgaaagt ctagatacta
     3421 taccattcca tgatataaac ttcttaagta tgtctgcagc acacctaata tattcatcac
     3481 cagcatcatg tccatatgta tcattaatat attttagatt atctaaatcc cacataataa
     3541 atgctgctat cttaagttca tctttattct taaatttttc ttgtatctgt gaatgaaaag
     3601 ctctcctatt taaaagattc gttaaaatat caaaatctct ttcatattct atcttccttt
     3661 tctcaataaa ttcttttgtg acatcaacaa caacaccaag aacctttagc tcatcttcaa
     3721 agatattcat tcttatccac ttaagttttt tatctttaga ttcaaactta tatatatatg
     3781 tattttctgt ttcataactt tcttgcaaat atttatctag tccccttaat tttttcagga
     3841 acttattttt ttccatatat ccatattctt caaatttgat ttcagtagta aacacgctaa
     3901 aaaattcttt tgtacaaaaa acattctctg cgtttttgta taactcaaaa gccccaattg
     3961 gcatatctac catttcaata atctgtgata attttgacga agaatctgca acatttgcac
     4021 tgagcaactc tatagctgaa gataattcat ctatttcaga tatatttatc ttatcaagat
     4081 ttactggttt tgatggatta ctgtttctaa ctttgttggc taaaagaaca attggttttg
     4141 taaataatct tgatgcaatg taaataccta ttagacctat taataaagaa attgctatag
     4201 atattataac taatttctct accctttttg aaaatgacaa aagacttttt tcttcaataa
     4261 tacccaaaag agcccaacgt tcattttcaa atggagtatt ggagttatat aaatccaagt
     4321 attgtacact agcataaatg ttgccattac cacattcatt atctttattt ttaatcttat
     4381 atatattttt atacgattcc ttctgctcta aattagaatg taaaccatcc caaaattttt
     4441 cttttaatga gtatccattt gaaattacat tttcaaaggc catatcatta tttctatcta
     4501 cacctaaaat ataattaccc tttttatttc catttatctc tgagtatgga agtaattctc
     4561 ttaaataatt tacacttaaa tctattccaa gtactccata tacagttcca tcttcataaa
     4621 taaggggaac tgtatatgtc ataagcctat cacctgcatt tgtcaataaa aacttacttc
     4681 cccagtatcc taaattagaa cttgatacat ttggattatc aattgcagct ctaaatggtt
     4741 tataaaaaaa atcatctcca tcttgctcat tttcactttt aaatgaaaac ttaggttccc
     4801 aataactgtc catagatatt ccaaaacttc ttgctatatc tgatgaacct ctctcaatta
     4861 acaaatcaga attatcattt ggattaaact tagggtctaa atcccttata tacaatcctg
     4921 tcctacttat ttcaccttta ctatctattg acttatcgtt aatatttttg tcacttagga
     4981 ttataaaagc tcctgtaaca gaattttttc tcaaaagata tattaaatca tttgatatgc
     5041 tttttataat atctttgtta atttcttcat tgttgcctac atcctcaatt aaggcatttt
     5101 tttcatttaa tacttttttt acagaggaat taattgtagc ttccgtttca cttgtatttg
     5161 accatctttg taacatttca ttttgtatat aatttttacg attaattact ctttcattta
     5221 agatgtcaaa cgaattatca tttaatttct taatagttcc tccccataaa attgtggcac
     5281 aaaataaact tgtttgaatt agcataacaa taattagggg aaatatcatc tttttcatta
     5341 ttgaattgtt ttttcttaga ttgttttcca tacccattcc acctatacct ttgtgctcgt
     5401 caacacacta actgccaatt catctatttt tttatagcat tttcaagctc agccttaaaa
     5461 ctttcaaacc aagttttaaa atttttatca ttattaaatt gtgctactgc atccttctta
     5521 gaagttccac tttttactaa ttcatttatt ttatttgaat cttctttagc tttatcaagc
     5581 attgaatatt caagtatttg tctagcttta gttccaccat caaatgcttt attagtatat
     5641 aaattacaac tgttaatctg ttcaatagat gaggctattg aaccttcaac tttatcagat
     5701 aaactcaaat tatctttctt cataacttct tttataacat ccaaatcatt cgtttttttc
     5761 tttactggta agtatccaga agcaatagag aattttatat taggttcttt atctgtaaac
     5821 cattttaaaa attctactga tgcatattct tcttctttag tagattttgt tacagccatt
     5881 cctgcacctt gttgtacagc atattttcct tctcctttaa aacgtggtgc ttgaaaaaca
     5941 gatgtttcaa tattataact ctcattatca tttagcatta ctctatctgg aaagtatgct
     6001 gctcctgaac tagaacctac aagagctaat atatcacctg tttttgcatc atcagaacga
     6061 aacttaccat aagaagcaaa atatccattt atatatggta catagaaatt atcccatagt
     6121 tttttcataa ctttttcatc aatatttagt gtaactttac catctttaac agaaaaaatt
     6181 tctactccta gttgtttact tcctataata atataatttg ctaaagcatc tcttccaaaa
     6241 aatgctttac catcattagg ctctttcgtt aaactatctg tccattcata atattttttt
     6301 gatgtctcta ctaatccttc aattgttttt atatcattta tatttgaatt tgtagctttt
     6361 gcaaatttat cccaatcagt tttatttaac atcaaaattt cagttgattt tgcaattggg
     6421 aaaagtttaa actcatcctt agaatttatt ctgccttctt ctacatattc gtctatgtat
     6481 tcatttaatt cttctttact tatgtactta tccatatcta ctagtaaccc taatttatcc
     6541 atttcatatg cagtatctga gtatgctgca aatatatttg gtacatcttc agaaccaact
     6601 tttttatttg cagaatctaa aactttctct gataaatcaa ctacattacc ttgactgaat
     6661 gcttcaacaa taattccttt ttcagcacct acagtgtcat taaattcctt aacaagctta
     6721 tcaaaagcca ttttctgctg tccattataa taatgccaaa tttgtattga tgtcggattt
     6781 tctgggtcta acttataatt atcttccttc tttaaactac atcctgtaag taataaaaca
     6841 atcattgatg tgataattaa ttttgcaaac ttacacctat tcataaaatc tactccttca
     6901 aattataaaa tttataatag cattaaaact gtaacttcac tatttatatt aattatagaa
     6961 cgtttattga tatatttaaa caggatttac aacatattta aaagcaatta agaaacatac
     7021 tattacaaat atagaaaata aagcttttac ataattttta tttctaaatg caccaaatga
     7081 caccaatcct tgttgaatag ctactgtaat tactaaaagt atcattatta tattaagttg
     7141 aaccagttta aataagtaaa gcaatgaaaa cactactaga agtacacata atacaatact
     7201 agatttatta ataatatcat caatcttatc tactttctta gattgcttat taattttatt
     7261 taatttttct ttatttaaat tctcaacttt tttattatac ctttctcttg ttttatcttt
     7321 atatttagta tttttatttt tatttcccat ttattttcca tcctctataa attttttaat
     7381 tgagcttata cattatttaa atattatata tttaagatac catatattcc tacaaaaagg
     7441 taacttacta tgtatctatt tttgattttc gcataaaaaa gccatctcct ttagaattaa
     7501 ttctgtattg agacagctaa tttaactatt tatcctttga aacattttct acattatcat
     7561 atttactatc aactaaaact atatcaactc ttctattttt tgacctacct tcttcagatg
     7621 aattttctgc tattggtcta aactcaccat atcctacagc agataattta tctggtgcta
     7681 tacctgcatt gtcaataaga agttgaacaa cattggttgc tctcataact gataaatccc
     7741 aatttgactt aaactctgaa ttttttatag acatattgtc tgtatggcct tctactctca
     7801 tgtaactatt catttcattt aacatcttac ctatttgaat aatcttatct ctagaattat
     7861 ccttcacaat agctttccct gtatcaaata gtattgtatc tttaaggctt attactaatc
     7921 ctctatcttg aactcttaat gatactgaat tagccatacc attttcgttt aaatacttct
     7981 gaatttcttc accaattttt ttaaacttag cattttgtaa agtatctaaa tcattacttc
     8041 ctggttcaac tactggttct atcttgctat taccaccttc aataactcca gaagaaccac
     8101 caaaagcaga atttagagat tgagataatt gtttatattt ctccgcatca acattactca
     8161 tagaatacat tattacaaag aaaatcataa gtaatgttat gaggtctgaa taagttaaaa
     8221 gccatcgctc attgttttct tctttttcat cttcatcata caacataaac attccatcct
     8281 tttatattat tcagctctac caccaatttc ttcaagttca attagttctt ttgaatttaa
     8341 aaatattttt aatttttctg tcattaaatt agaattatca ccctcaagta tggataatat
     8401 tgcttctatc accaaaagtc tttcacttac ttcttgttga tttatagcct taagttttga
     8461 tgctattggt aaccatatta aattggcaga accaacacca tacaaagtag ccaaaaatgc
     8521 aacagaaatt tgaggtccta atgcatctgg attacttgaa agattaccta aaatatgtac
     8581 taatcccata actgtaccta ttatccccag tgtaggtgca tatccacctg ctgaatcaaa
     8641 aattgctatt ccttctttat gtcgttttga tgttatctgt atctgtgatt ccaatatatt
     8701 tcttatagta actgcatcca ctccatctac cataagttca agcccttttc taataaaagg
     8761 gtctaattca tcattctcac ttatttcttt gtcaatacta agaagcccct ctttcctagc
     8821 ctttgttgca atttctttga aatagattat attttctatg ttattaggct ttttattttt
     8881 aaaagttaat aacattatct ttatagactt tttaaacaca gggaatggag tcgaagctcc
     8941 aacagctcct atagttccac caaacacaac aagtgctgat gaaaatacaa ataatgctcc
     9001 aacatgtccc ccatcaataa caaaagcaac taccaaagat gctatcccca ctatgaaaaa
     9061 aattaaagtt gtcatctatt ccctcctttc tttagttata catatctaat catttatgac
     9121 attataaaaa gactatcttt aataaaaata gcctttaata attggcggag aaggagggat
     9181 tcgaaccctc gcaccggtta cccagcctaa tcccttagca ggggatcctc ttgagccact
     9241 tgagtacttc tccatatctc tatactagta aattatataa taaaatcata ttttagtcaa
     9301 ttgttttata aaatttgagt tattttgaat tattaaacat ctatttttac ttttttgctt
     9361 aaatgtaagt tttaaatatg gtaaaattat cttgcataac acttataata ttatttgata
     9421 aatatacata cattaacaaa tatacctaaa ctctgactta attgtaagtt atgccaaaac
     9481 taaaataata ctagtccaag gaggatattt tatgttacca caggaaagat atgagaagat
     9541 tttaagtatt ctcaaatcag ataatataat taaaataaat gacatagttc aaatgtttga
     9601 tatatcaata gaaactgcac gaagagattt agcccatatg gagagtatcg gtcttttaaa
     9661 aagagtttat ggtggtgcta taccagttat agcaagtagc gtagaactta actatgttaa
     9721 aagaaaaaaa ataaactcaa acgataaaaa acagattgct attaaatgta gtgagtttat
     9781 aaatacaaat gatactattt ttatggatac tggaactact gtacttgaag tttcaaaaaa
     9841 tttgaaagat aaaaagaatc taactgtaat aaccaactca attttagttg caaacgagct
     9901 tatgaatact aatataaaag tatatgtgct tgggggctat cttagagaaa ctgaaggttc
     9961 tttttctggt ccgcttacac tgtcagctat tgaacaattt aatgttgata aagctatttt
    10021 aggcgctgga ggtatcactc ttgaaaatgg aatctctgac tatcacttag aagaagcact
    10081 tgttagaaaa aaaatgattg aaaggtctag aaaatctata gtagttgtcg aacctcctaa
    10141 gtttggtata aattcatttg catcagtagc tcctatagaa catatagata ctattattac
    10201 ctctgctagt atagaccata atataataat agagttttct caaaaaggaa tacctttagt
    10261 catagctgat aattagctta tatgattgat ttttagttcc ttgatgtaat aaattaaaat
    10321 taaggagcca aataattatt tatttagctc catttatgtc taaactatac tttatatttt
    10381 aattatgtaa ctactttcag ttagcttatc atttttagtt atttgattgc cattttattt
    10441 aatttataag ttttagttat tcgattattt tttaattgtc tgtcttttta atttattttt
    10501 agttatttaa actcctactt taaaataatt taaatttgac ttaagtattg tttattctac
    10561 actgcatctt tcttcatttt aatactttgt tcttcttcca aggttaaatc ttttgtcata
    10621 agtatagcaa gtgcaattgc caatactcct ccaaccaatt ttccaattac aacaggtgta
    10681 atcatctctg gaactactcc tgctgtaaat cctaaatggt cccccaaagc tgctgtagca
    10741 caaaccagcc aagcagtatt tactactttc ccctttggac tcatatcttt catcatttta
    10801 tatactggaa tactattggc taaagtaaaa actaatcctg ctgcactagt ggcatccata
    10861 cctaattttc cacccactat tgtaagtggt ttattaagaa ctttaactaa taaatgtaat
    10921 attggaaaag tacctaataa cacgattgca atacttccaa ttacttgaat accttctctt
    10981 ataggtgtca tgccttttat aagtactata cctgttattt cttgaaatgc tgctgctccc
    11041 aaacctatcg ttatcaatat agatataaat tttccaaaaa ctaatgaccc atttatcata
    11101 agcgtagaat taaattttaa acctactgcc aatattactg ccaatattat cactggtatc
    11161 atatttataa ttaccattga taagttaaat cctgctatta atcctcctat aatacctcca
    11221 aaaggtattg ttatcaagcc tatcagtaac ccttttgcaa agaatggtct gtcttcatat
    11281 tctataagac ctagtcctac tggaattgaa aatactaatg tacatccaag catggatgac
    11341 acaaccaatc ctgcaagcat tcctgcctct ttattgtgtg caagttccat agccaatgga
    11401 taccctccca tatcattcgc taaaatagtt gcaaacatag atgggtcagc tccacagaga
    11461 ttgaagattg gtattattat aggacctagt acatctgaaa ttactggagc taaacaaact
    11521 ataccaacca ttcctaaagc caatggaccc attgaattaa acccttcttc aaactgctcg
    11581 ccaagtccaa atttatttcc aattattcta tctaacgctc ctaaaagcac tcccacagcc
    11641 attatgtata atataatttt atcaaagggc atttttaatt cctcctatac atgtaaaata
    11701 ctctattgta acttaattag cacacacctt tttggtatcg ttttcccaat tcatagctct
    11761 ttttactgct tggttccatc caagtaatat ttcttctctt ttttgagtag tcatatttgg
    11821 aatatatttt ttgcttattg taaattcact tctaatttca tcaatactgt tccagacacc
    11881 acaagaaaga cctgccatat atcctactcc aagtactgtc gtctcaggat tttctgcaat
    11941 ttcaattggt atacctaata tgtcggattg aaattgcatt gtaaagttat ttttagtaat
    12001 tccaccatct actctcaaca tttttatttt tttccctgag gctaagttca tagaatcaac
    12061 aacatctttg acttgaagtg caacagactc aagagtcgct cttactatat gctctttttt
    12121 agtacttcct gttattccaa ccattgtacc tctagcatat tggtcccaat aaggtgctgc
    12181 tatacctgca aatgctggta caaaatatac acctccagta cttgatatgg aatttgccat
    12241 gtcatcagtt tctgaataat tttttataac tccaatgcca tctctaagcc attgtattgc
    12301 agcacctgct atatacactc caccatcata agcatatgta actttatctc ctattttcca
    12361 agcaacagtt gtcaaaatac cattacctgc tatatttagt tcctcaccta tattcatata
    12421 taggaaacat cctgtaccat aagtattttt aaccattcct ggttcaaaac atccattgcc
    12481 aaataaagct gctggttggt ctaccattga agcagtaatt tttatagaaa ccccacaaaa
    12541 tgcctcctta tctgtagttc caaaatcacc actcgtaggc attatatctg ctaaaatact
    12601 tctaggtact cctataatgt caagcaactc tctatcccaa tccatatctc ttatattaaa
    12661 aagcattgtt cttgctgctg tagatacatc agttaaaaaa gattttcctc ctgtcatttt
    12721 ccatattaac caactatcta atgttcctgc aagtacttct ccatttttaa ttttttcttt
    12781 tacacctttt acattatcta ttacccattt aatttttgta cctgagaaat atgggtctat
    12841 aattaaccca gtcttatcta ttatcttatt ttctaaacct ggtatattct ttaaattttc
    12901 cacataatca taagttctcc tgcactgcca cacaatagca ttgtaaagtg gtttacctgt
    12961 atttttatcc cataacatta ctgtttcacc ttgattatct ataccaattc cttttatatc
    13021 actttcattg attccatttt caaaagcctg ttgtaataca ttattactca cagtttttgt
    13081 attattccaa atttctatag ggtcatgttc aacatatcca gattgtggat atatttgagt
    13141 atgttccata taatcgctaa aaacaaactc tgatttttcg tttatcaata ctgctcttgt
    13201 tcctgttgtt ccttggtcaa ttgctaatac atatttcact ttaaatgtcc ccctttattt
    13261 tcagaattaa taaattcttt tttatttttt gaatatataa tctattcaag ttatttatat
    13321 tttatatcat catgtggatt tatgcaatat tcattacaat attttttgtt atttttaatt
    13381 atagtgatta aaaaagtatt tttattgaaa tattattgca atatttatta ttttattcta
    13441 taatctagtt aaaataacac ataatatttt aatatttaca cataatcagt cattcaaaaa
    13501 tttaaattta actttttaag gaggctttac aatgaaaaaa acatatctat tcagtcctgg
    13561 accagtaatg gttacagata aagtcagaaa ttcacttctt cactatgata tatgccatag
    13621 aggaaatgag tttatggatt tattcaaaga tacacaacaa aaaatcaata aactatataa
    13681 tgcaacttct aattactact cattaattgt atctggctct ggaacatctg taaatgaatg
    13741 tgtactatct tctatctttg acaaagaaga tgcagttctg ctagttagta atggtgtttt
    13801 tggagaaagg ctagaagaaa tattatctac ttataatgta aaaacatata aacctaattt
    13861 tgaatgggca gaatacccgg acctagacat tttagaaaaa tacataattg aaaattctga
    13921 tataaaagta attgctatgg tgttccatga aactagttca ggaatgataa atccagtccc
    13981 tgaactagga aaacttgcta agaagtataa caaaatattt tttgtagatg caataagtgc
    14041 ctcagcaggt gaatatattg atgtggaaga gtttaatata gatataataa caggtgttgg
    14101 tggcaaagct cttggagcat ttccaggttc tgcatacctt tgtgctaaag agagtatttt
    14161 acaaaatatt aaagaaaatc aatgtaagaa tgtgtatctt aatctttata aacactataa
    14221 agttgctaaa tcatcttctc aaacaccaaa tacacctaat gttactttaa tatttgccct
    14281 taatgaagct ttaactgagt ttttggaaga tgacagtaaa atagaaagat acaaagaatg
    14341 ttctgctatt ttaaggaatg gtatggaaga attgggtcta acatttttac ttccagataa
    14401 gtatatgtct aatacagtaa catctgtatt tctacctaaa gaaatcaatg taaatgagtt
    14461 tatattaggg ctagaaaggg aaagtggcta tgttgtctat ccaggaaaag gtaaattcct
    14521 agatgctaat atattccaag tagcaaatat gggagaaata tatccagaag attgttataa
    14581 attcttagat atcttaaaat caaaattgga gataaaaaaa gttatataac agtttgtttg
    14641 taaaatccta caatatatca gtataaatta aatagactga tttttttaaa ttttcctata
    14701 ataaattata ttttatattt ttgtataagg gtgaactata atatgtaaag ttcaccccta
    14761 tttcaaatct ttttccttca atataatctt tttcttttaa tttattttat atttttgaca
    14821 taattattct tacatttaat tgttttttat ttttgacgca atcgtttttt attcagtcat
    14881 tttatattct tgatacaatc attcttacac ttaattattt tttatttttg acacaatcgt
    14941 tttttattca gtcatttaat attttcaaca caatcattct tacattctat tattgtttgt
    15001 tttgatacaa ctgtttttat tcagtcattt tatattctca acacaattac cttttcattc
    15061 aatcaaagta atctttaagc attatttttc aagctcatca aagtaattat atacgataac
    15121 ctcacctgtc tctttatctg tagtctttat aatgccctta gctccattta cctcttcttt
    15181 tgtaaattca tcatctaaca caactttgaa tttatcttcc tttccataac cttgttgaaa
    15241 ttcatctctc aaaattgcat ttttttctgc ttttggcttc aactcaaatt caattccaga
    15301 tttcatttca tcaaaataat ttaatactac aacctcatta ttcattttat tagttgtctt
    15361 tataatccct ttagctccat ttacttcttc ttttgtaaat tcatcatcca atgttatatt
    15421 aaatttgtct tcttttccat attcctcttg aaaatccatc ttcatttcat tgtatttagt
    15481 ttcaaactct ttactcttgt ttaattccat agctgataca tttgtcaatg atgctccaat
    15541 tgccattact gttaatgcca ttactatacc acttttaaat ttttttactt tcatatctta
    15601 cctcctaaat taaatgttct caatgaaatt atataaagaa aatcctaagt tttacagttt
    15661 cattcttgta actaaaatta ctattatgta accaaacttt ttgcttcaac tattccttaa
    15721 aagttgcttt tttgaggaat agttgaagtt aaataattag attttatcta tatttataga
    15781 tttaaaatca aatttatcca tataaataaa tacttatttt tgcaaaataa aaatttttaa
    15841 ttactccaat ccttcttatt tacttaaaat cctatgataa gaaatttaaa agtctttacc
    15901 ttctttaaca ataatttatt tacttttaac atacaaaata tagagagtat atcaaaatca
    15961 ttttactgaa tattctaaaa ataagtaccc cctattagta tatatattta catattaaaa
    16021 aaaagtttta aataaagtta tattttttac aacagttttg taattaaaaa catattgaag
    16081 gtaaatttaa aggtactata tactataaat atgtcgacac aataaattta aaaaggagaa
    16141 ttaaatatga aaaaaagatt aataataatg atattgtctg ttgtcttagt actatcaagt
    16201 atacttacga tatttgcata ttcaaatatt aaatataata ataataaatt gatatattct
    16261 aatatgatag ataaaaaaac tcaaaatagt gtaaaggaaa tcctaaaaga aaataaaatt
    16321 aatgaaaaag acatagatac atttattaaa gcagtcaaca attataataa attgcaagtt
    16381 aaaatacttc aaaataatat aaatatatct aaatctggat attcttcaat caatgcaaaa
    16441 caagttccat ataacctaga aaaattgcaa gataattggg tgaaaaaatt ccctgattat
    16501 atggatgtaa attgtagaat aactgctttt agacttttta aagattttat aaattcaaat
    16561 aagaaattta ctggagactc tattgatttg aatgtcgact tggacactat aatgaataat
    16621 aaagatgcta aatttagcat caaagatgta gaaaaattta tcaacttctt ttctgcaata
    16681 ccagctaaag atacagatga tacaatcaaa atttcagaac aaataaaaaa tgaatggaaa
    16741 aaaagaaaaa tatcttttaa aaataataaa aatatatcta ttataaatgg atttcttcgt
    16801 tatccagaaa ctaaaaacgt atttataggt catacaggca tatgtattaa aactaaaaat
    16861 ggcattctat ttttagaaaa atacggtgtt acatcacctt atcaagttac taaatttaaa
    16921 aataaagaag atgtaaaaaa ttatatgttt aatagattga agatgtcaga aggtgaaatt
    16981 gaattacctg accctataat aatggaaaac gataaactta tgaaataata actttaaacg
    17041 caaaataata tttataatta ctaagtcaat ataatagaga aggtctatag atttgtgtaa
    17101 atacaa
//
LOCUS       DAMJWU010000078        16815 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMJWU010000078 DAMJWU010000000
VERSION     DAMJWU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.080"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            293..751
                     /locus_tag="PC460_002673"
     CDS             293..751
                     /locus_tag="PC460_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2601551.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            812..1087
                     /locus_tag="PC460_002674"
     CDS             812..1087
                     /locus_tag="PC460_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2601552.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1146..2420
                     /locus_tag="PC460_002675"
     CDS             1146..2420
                     /locus_tag="PC460_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2601553.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2550..3602
                     /locus_tag="PC460_002676"
     CDS             2550..3602
                     /locus_tag="PC460_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2601554.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3602..4633
                     /locus_tag="PC460_002677"
     CDS             3602..4633
                     /locus_tag="PC460_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF2601555.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4725..5537
                     /locus_tag="PC460_002678"
     CDS             4725..5537
                     /locus_tag="PC460_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2601556.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5540..6460
                     /locus_tag="PC460_002679"
     CDS             5540..6460
                     /locus_tag="PC460_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2601557.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6635..7081
                     /gene="rpiB"
                     /locus_tag="PC460_002680"
     CDS             6635..7081
                     /gene="rpiB"
                     /locus_tag="PC460_002680"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2601558.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7111..7776
                     /locus_tag="PC460_002681"
     CDS             7111..7776
                     /locus_tag="PC460_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2601559.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7886..9388
                     /locus_tag="PC460_002682"
     CDS             7886..9388
                     /locus_tag="PC460_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF2601560.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRTYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9700..11289)
                     /locus_tag="PC460_002683"
     CDS             complement(9700..11289)
                     /locus_tag="PC460_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2601561.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSNI
                     NYYYREFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVKF
                     LENVINLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIYE
                     NIKYNNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFM
                     YIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVI
                     GDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDML
                     KNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQS
                     IVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDM
                     FIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENA
                     HMEIIIDADLFKVMLTFRKVN"
     gene            complement(11258..11974)
                     /locus_tag="PC460_002684"
     CDS             complement(11258..11974)
                     /locus_tag="PC460_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2601562.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12513..13313)
                     /locus_tag="PC460_002685"
     CDS             complement(12513..13313)
                     /locus_tag="PC460_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2601563.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(13627..14358)
                     /locus_tag="PC460_002686"
     CDS             complement(13627..14358)
                     /locus_tag="PC460_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601564.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(14345..15040)
                     /locus_tag="PC460_002687"
     CDS             complement(14345..15040)
                     /locus_tag="PC460_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2601565.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(15042..15833)
                     /locus_tag="PC460_002688"
     CDS             complement(15042..15833)
                     /locus_tag="PC460_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601566.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            complement(15843..16655)
                     /gene="modA"
                     /locus_tag="PC460_002689"
     CDS             complement(15843..16655)
                     /gene="modA"
                     /locus_tag="PC460_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2601567.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         6405 a         2064 c         2709 g         5637 t
ORIGIN      
        1 gttaaacata aaaaaccatt tgtaaaaaac aaaaaaatca tttctctaac aaaaaaaacc
       61 acaaaaaata taaaaaagta tggctagtta agtgattaac aagtaaaaat taaaataaaa
      121 atcaaaaaaa atttatcaat gaaagtcagc taaaatcctg gaagagagca ttatttttgg
      181 gaaaacattt tcttttgttt cacaaaaaaa gccacttttt ggcacacaac atgctttata
      241 taaagtcaag aaaaactaag gtataagaca aaggcaatta ggaggtgtaa atatggagtt
      301 tataaatttt gtaagtgata aagttataga ccttaaaatg gattgtaaaa cgaaagaaga
      361 tttctttaaa gaaatacata ataaagtttt tgatttaggc tttgtaaaag aagaatttgg
      421 agaaaaaata ttagcaagag aaaatgtatt tccaacaggt ttaaatttag gagattatgg
      481 agtagcaata ccacatacag atgcagaata tattaaagag caatttatat cagtctgtac
      541 ttttaatgaa ccagtagtgt ttagttctat ggaagaccaa gatgaaaaag tacctgtaaa
      601 tttggctttc gtattaggac ttaatcaacc acacagtcaa ttatctgtat taacagaatt
      661 aatgggaatt atgcaaaata aagagttagt tgataagttg atgagttcta ctgataaaga
      721 agaagtttta aagacactaa agtctttata aaaataaaaa agtactaaaa ataaaaaata
      781 taaaaataaa aattattagg aggaaataaa aatgaaaaaa atattagtag catgtggagc
      841 aggaatagca acatcaacag tagtttgtga caaggtagag agattagtta aggaaaataa
      901 tatacaagca gaagttatac aatgcaaaat agctgaatgt gcaacaaagc aagaaggagc
      961 agatttaata gtatcaacaa ctatactacc aacaacttat aatataccag caataaaagc
     1021 aacagcatat ataactggag taaatacagc agcattagat aaaaaaatat tagatgcttt
     1081 aaaataatcc tatcatagat acattgaaat aatattttat aaaacagaaa aaggaggatt
     1141 tataaatgga taaattatta gcagtagtac aatacatttt aaatatggga ccgacagtaa
     1201 tactaccaat aacaattgta gtactaggaa tgatatttag aattaagttt acaaaagctt
     1261 taaagtcagg tcttacaatt ggtataggat ttgtgggtat aaacttagtt gtaaacctat
     1321 taacatcaac actagggcca gcggctcaag gaatggttga aagattcgga cttaatttaa
     1381 cagtaataga tgttggttgg ccaacaacag catcagcaac atgggcttca ccagttgcac
     1441 caatattaat accagtttgt ttattagtaa acttactatt attatacttc aataaaacaa
     1501 agatattaaa cgttgatata tggaattatt ggcactttat agtagcaggt gcagttggat
     1561 atatagtaac aggaagttat gtttgggcta tattctgtgc aatactaatg gagttattag
     1621 tactgtttat tgcagaaaag acagcaaaaa atgttcagga gttttatgat ttagaaggta
     1681 tagcattacc aactggttca acagcttcat ttgctccact tggatattta gtaggtaaat
     1741 ttgttgaaaa aataccagga ataaataaaa tacaagcaga cccagactct atacaaaaaa
     1801 gatttggtat atttggagaa ccaatgatga tggggattat actaggtata atacttggag
     1861 cacttgcggg atatgatgta gcaggaatat tcaatgtagg tatatcaatg ggtgcagtaa
     1921 tgttacttat gcctagaatg gttagaatat taatggaagg tttaattcca atttcagaat
     1981 caataagaga tttcttacaa gcaaaatatg gagacagaga actttatata ggtctggatg
     2041 cagcagtagc aactggacat ccagcagtta tatcaacagc tcttatactt gttcctataa
     2101 ctttgttctt agcagttata ctaccaggaa acaaagtttt accatttgga gacttagcga
     2161 caataccatt ttatatggca tttatagtgt gttttagaaa aggtaatata gttcaatcag
     2221 ttataacagg aacaatagta atagctatat cactttatat ggctacaaac tttgcagatg
     2281 ttcatacaca attattacaa caagcaaata tgatgccaga aggaacaaca atggttacaa
     2341 gtatagatac aggtggaaac ttgctaaaat ggggaatatt gaaattatct caattagttt
     2401 cttcatttgg aatattctag tatgtagtta atagagatta ctctttttta gtaaagtatg
     2461 cgaaaattta acatttagca gctactaaaa aagagtaata ttttttataa atttttaaaa
     2521 atacaaaata caagaggagg agatttatcg tgaaagcagc agtattacat ggcacaaatg
     2581 atatgagatt tgaagatata gaaataaaac catgtgaaag tgatgaagta aaaataaaag
     2641 ttatggcagc aggaatatgt gggtcagatc ctccaagagt attaaaacat tggaaatatc
     2701 cagttccagc tataccagga catgagtttt ctggagtaat tgctgaagtt ggtaaggatg
     2761 taaaaaatgt aaaagttgga gatagagtag ttgcaattcc ttttatacct tgtaatgaat
     2821 gtgaatattg taaaagagga ttattttcat tgtgtgatga ccatggaatg ttaggagcta
     2881 aaagttttgg agcttttgca gagtatgtaa atataaaagc tacaaatgta cttccaatag
     2941 gagatatgaa ctttgaagat gctgcaatga tagagccact agcagttgct atgcatggag
     3001 tacttaatat aggtgttcaa gttggagaca ctgtagctgt aatgggtagt ggaacaatgg
     3061 ggcagttggt aatacaagga cttaaaatag caggggcagg aacaattata gcagttgata
     3121 tatctgataa taaattaaga gaatcaaagg aattaggggc tgatataata attaatgcta
     3181 aagatattaa cccagtagaa aaaataaaag aacttactgg tggaaaaggt gttgacatag
     3241 ctcttgaatg tgcaggttca aaaataactc aagaacaatg tttacttata actaaaaaga
     3301 aaagtaagat aggattttta gggatagcat attcagatat aactttatca gaagaagcat
     3361 ttgaaaatat atttagaaaa gaactagaat taaaaggttt ttggaactct tattctgcac
     3421 catttccagg acaagaatgg actaagggaa tcaatctagt taatgaagga aagataaaat
     3481 taaaagaaat ggtatctcat agattctcac ttgaagatac ttacaaagca tttgaaatga
     3541 taagagatag aaaagaagaa tttaacaaaa tattaatttt accacaaggg gttgaaaaat
     3601 aatgaaagca gtagttaaaa ctaaaccagg ctatgataat gtagaagttt tagaagtaga
     3661 agaaccaaaa gctacaggag acaaggttaa aataaaagta gaatatagtg gtatatgtgg
     3721 ttcagatata cattcattta aaggtgaata tgcaaatata aaagccccag taacattagg
     3781 tcatgaattt tctggaattg ttgtagaagt aggagaagat gttaaaaata taaaagtagg
     3841 ggatagagtt acatcagaga ctacttttgc aacttgtgag aaatgtatat attgtgaaac
     3901 taaagattat aacttatgcc caactagaaa aggtattggt actcaagtaa atggaagttt
     3961 tgctgagtat gtattatcaa gagaagaaag catacatgta cttcctgaaa atgtatcttt
     4021 attatcagca gcccttacag agccactagc ttgttgtgtt catgcagctc ttgaaaagac
     4081 tactatagaa agttctgata cagttttaat aataggacca ggaccaattg gtttattatt
     4141 agctcaagtt gttaaatctc aaggtgctac agttataatg tctggtgtta caaaagataa
     4201 ggaaagatta gaaatagcta aaaatcttgg agtagataga actgtaaatg taatggaaga
     4261 aagtttggca gatgtagtga atgaaatgac tgatggaaat ggagtaaaca aaggatttga
     4321 ttgttctggg tttatgccag cagttaacga agccttaaga ctgcttagaa aaaaaggtac
     4381 tttcgtacaa gttggaatct ttgctaaaaa attaaatgaa atggaccaag aagcaataat
     4441 ccaaagagag ttacaatata taggaagcag gtctcaaaag ccaagttctt ggattacagc
     4501 attagattta atggcaaatg ggaaagtaaa tacagaagct ttagttacta gaactgttgg
     4561 tttagacgaa tttaaagaag gtattacagc tttaatgaac ggggaagaaa taaaagtagc
     4621 agtaaaatct taaaaaaaca tttgtattat tttaaaaaaa attgtaaaat ctactgtagg
     4681 ataaaaaaat gtaaggtctt aaaaaataac aggaggaaga aagtatgagc actcttagag
     4741 atcattctaa taatattaga aaaaacataa taaaaatggt tgcagaagct aaaagcggac
     4801 atccaggtgg ttcattatca atagctgata tgttgacagt attatatttt gataagatga
     4861 acgtatgtgc agaaaatcca aaaatggctg atagagatag atttgttcta tcaaaaggtc
     4921 atgcagcacc agctttatat gctacattag cagaaaaagg atttttccca gaagaagaat
     4981 taataacact aagaaaattt ggttctaagt tacaaggtca cccagacatg aaaaaagtag
     5041 caggaatcga tatgtctact ggttctcttg gtcaagggct atctgcagca aatggtatgg
     5101 cacttgcagg aaaattagat aacaaagact atacagtata tactatatta ggtgatggag
     5161 aaatccaaga aggtcaaata tgggaagctg caatgacagc atcacattac aaattagata
     5221 atttaattgc atttgttgac ttaaatggat tacaaataga tggttctaat gaagaagtaa
     5281 tgaatgtaag tccagtagat gacaaattca aatcttttgg atggaatgta atagtaataa
     5341 atggacattc ttttgaagaa ataggaaatg cagttgatga agctaaaaaa gttacaggaa
     5401 aaccaactgt aataatagct aaaacagtaa aaggtaaagg tgtttcattt atggaaaata
     5461 atgctgcatg gcatggaaca gctccaaatg cagaacaagc agagcaagca ttgaaagatt
     5521 tagaaggagg tctataggaa tgggaatagc aactagagaa gcttacggac aagtgttaaa
     5581 agaacttgca gaaaataaag atatagtagt attagatgca gatttaggaa aagctacaaa
     5641 aagtatatca tttaaagaag ttgccccaga tagatttttt gacatgggga tagcagaagg
     5701 tgacatgatt ggtacagcag caggtcttgc tacttgtgga aaaatacctt ttgcgagtac
     5761 ttttgcaata tttgcagcag gaagaggcta tgagcaaata agaaactctg tagcatatcc
     5821 aaatttaaat gtaaaaatag cagctactca tgcaggtgta acagtaggtg aagatggtgg
     5881 tagccatcaa gctatagagg atatatctct aatgagaggt ataccaaata tggttgtatt
     5941 aaatccagca gatgctttag aagcaagaca agctattctt acatctattg attataatgg
     6001 acctgtttat ataagattag gtagagctgc aactcctgat gtaaacagtg aaaattataa
     6061 atttgaaata ggaaaaggaa ctgttttaag agaaggttct gacataacag taatagctac
     6121 aggaataatg gtagcaaaag ccttagaagc agcagaagaa cttgctaaag aaggtataaa
     6181 tgtagaagtt gttaatatat ctacaataaa accattagat gaagcattaa taaaagaaac
     6241 tgctaagaaa actggaaaag tagttacagc agaggagcac agtataatag gtggtttagg
     6301 ttcagctgta tgtgaagctt tagcagaaac taaagatgta gtagttagaa gaattggtgt
     6361 taaagatgta tttggtcaat caggtacacc agctgattta ttaaaacact atggtttaac
     6421 tacagaagat atagtaaaaa atataaaaga gttgttatag atataaatat atctaaataa
     6481 caaattgatt tgacataagt caattaaagc tgtcttaaaa atagaaagaa atttctacaa
     6541 tgggacagct ttatttttac aatactttat ttttatgata attataaaat agtctgaaag
     6601 taatcaaaaa aattataaaa agaggtgcta tttaatgaaa atatctattg gatgtgacca
     6661 tggtggttat gaattaaagg aatatataaa ggaaaattta ataaataaag gtatagaagt
     6721 tgaagatgtg ggtacaaatt caaaagaaag tgtagatttt ccaatatatg caaaaaaggt
     6781 aagtcaaaat gttcaaagta aaaaatctga tttaggcata ctttgttgtg gaacaggaat
     6841 aggtatgtct attgcagcaa ataagtttaa aggaatcaga gcagctgttg tttctgactg
     6901 tttttcagct aaggcaacta gagaacacaa taatacaaat gtactttgtt taggagaaag
     6961 agttattggg aaaggattag caatgagaat tgttgaagaa tggttaaatt caagttataa
     7021 tggagaaaga catgcaagaa gattacaaat gattgatgat atagaaaaag aaaatttata
     7081 aatatataat ttacaacaag ggggagcgtt atgtctatta tttgtgcttc aataatgtgt
     7141 gcagaccaaa tgaagttaaa agaggaatta gaagctcttg aagaagcagg tgtaaaatta
     7201 cttcattgtg atgttatgga tggaatattt gttaaaaact tggctatggg accagagtta
     7261 ttaaaatcaa taagtgaaaa tacaacaata cctttagaca tacacttagc cacagaaact
     7321 ccagataaat atattgatat gatgtcttat ataaagccaa aatatatttc ttttcatgta
     7381 gaatcaagta caaatgtaaa agctgatata caaaagttaa gaaattatgg tatagggcca
     7441 gtgttagcaa taagccctca aacaagtgtg gataaaatag aagaatatat aagtttagtt
     7501 gagggtatct tagtaatgac agtaaatcca gggtttgcag gtcaaaagtt taatctttct
     7561 gtattggata agttggataa acttacagag atactaaaag attatgataa tcctccattt
     7621 atagaggttg atggaaatat aaataaggat actattaaac ttatgaatgg taaaaaagta
     7681 gatatatatg ttgttggtac ttcagcttta tttaatgata agcctccaat ttcatataaa
     7741 gataaaatag aagaactaaa agaaagtata aagtaaccat gtaataaaat tataatatgt
     7801 ttctgtttaa tagtaaaaat ttaggtggaa acaagaaatt ttataatatt taataaaaat
     7861 tttggttgag taaggagaat tttttatggg agacctatat aaactacaaa atggtactga
     7921 tattagagga atagcgtatg aaaatgattc taaagaagtt aacctaactg tagaagaagt
     7981 aaaaaaaata gctaaagcgt ttcatatttg gttaaaagaa aagactaaga aagataaagt
     8041 aactgttgcc ataggaactg attctaggat aacaggaagc caatttagaa gtactgttat
     8101 tgaaacttta actaatgatg attgtaatgt aattgattgt gagattgcca caactccagc
     8161 tatgtttatg acaactatta tggatggata caattctgat ggggcgatta tgattacagc
     8221 aagtcactta ccttattact ataatggttt aaagtttttc acagaaagtg gaggtttaga
     8281 aaaaactgat ataaaagaga tgttggatat agctgttaaa aatgatagtg tagactatga
     8341 ggaagtaaat aaaaaaggtg aagttatagt aaaaaattta atagaagatt attctaattt
     8401 attgatagat aaaattagaa aaggtgtaaa ttcaagtaaa aattatgaaa agcctttttc
     8461 tgggcttaag atattggttg atgcaggaaa tggggctggt ggattctttg ctgaaaaagt
     8521 tttacacata ctgggagcag atacgacagg tagtcagttt ttgaacccag atggtatgtt
     8581 cccaaatcat atacctaatc ccgaaaacaa agaagcaatg gaaagcatat gtaaagctgt
     8641 gttggataat aagagcgacc taggaataat ctttgataca gatgtagaca gagctgctat
     8701 tgtaggtaaa aatggcaaac caataaataa aaatgctctt atagctgtta tatcatcaat
     8761 agtattagaa gaacatccaa aaacagctat agtaacagat tcgattactt ctgaaggact
     8821 tgctaaattt ataaatgaat tacaaggaag acatcataga tttaaaagag gatataaaaa
     8881 tgtaataaat gaagctataa gattaaatag tgaaggtgaa gagtgccatt tagctataga
     8941 aacgtctgga catgctgctt taaaagaaaa ttatttctta gatgatgggg catatttaat
     9001 agctaaaatt ttaataaaag tagcaaagct atccttggaa ggaaagacta tagaagaatt
     9061 gatagaaaac cttgaagaag ctcaagaaga gaaagaagtt agaataggca ttaataaaga
     9121 agattttaga acatatgcag aggatatatt aaaagattta gattctcatg ttaagagtat
     9181 tgatggatgg tctgtagcac caaataattt tgaaggcata agagttaatt gtgatgaatc
     9241 aaatggagat ggatggtttt tacttagaat atcactacat gaaccattat tagctttaaa
     9301 tattgaatca aataaaattg gcggagcaga acaaatttat tctaagttaa aacttttctt
     9361 agaaaaatat gatttaaaag gtctttaggg taaatagaaa gaatatattt atattgaaga
     9421 ataaatgtaa atttgtacct aagattacaa atattacaaa attgtaatct taggtaacag
     9481 agatgtaact gattatggaa acactatgtg atattataga tacagagaga tgaaatatgt
     9541 gatgaattat agagaaatat aatttaaaat gtattaatat cacaccaaaa aggtataatc
     9601 aaaaaatatg taaatataaa ccccaacatt tagaaaaaga cctctaagga ggtttttttc
     9661 ttgtttaaat aacttaaaat atatttttat attaatatat caattaactt ttctaaaagt
     9721 aagcattacc ttaaataaat cagcatctat aattatttcc atgtgtgcat tttctaattc
     9781 aattatattt tgagcaattg ataaaccaag accagagcca tctgtttttc tagatatatc
     9841 accccttcta aatctttcta ttaactcttc tggttttaga tttagtttat catttgaaat
     9901 atttttaaac gttaatatta tattttcttc ctttataaac atatctatat agaccctagt
     9961 gttaggcata gagtatttta gtatattgca aattagattc tcaaaaactc tccaagtttt
    10021 tttaccatct atcatggtaa aaacagcata ttcaggtgtt tctattataa aatctaaact
    10081 gctattttga attttgtctt caaattctac aatactttga attaaaagtt ctcttatatc
    10141 ggtttttgtt atattaaatt ctacatctcc actacttagc ttagatgctt caaatatgtc
    10201 atctattaat aattttaatc tttgtgattt tttatctaaa atatttatat agtcatttat
    10261 agtctctctg tcaaagtcat tattttttaa catatcaaca tagcttaata tagatgtaag
    10321 tggagttttt aagtcatgag atacgtttgt gataagctct gccttcatcc tttcattttt
    10381 tatattagca tctacagcag tttcaagacc gcctgttata ttatttatac ttttggctaa
    10441 agtagatatt tcatcatctc ctataacttc taatttatac tttaaattgc cattttctat
    10501 attttttata ttttttgata tgttatttat gtaattaaac ttaacagata aaaagttgta
    10561 tatataaaaa tgaaaaactg tgtttacaag tgtaaataaa attactatag ctattgcctc
    10621 agcataatat atatacataa agaatatcaa aacaagatat tggataatag cttgtacaac
    10681 tatagatatt aaaaatacta tagctaattt tttatttata gtttttttat tatacatact
    10741 aagtaagttt gaaaagaata catatgttat agacatatct tttagattat agttattata
    10801 tttaatattt tcatatatta tatagactat agatataatt ataaaattgt agattattaa
    10861 attagtgttg taaaacattt tattacttgt aaatgcatga aaccttaagg ttaatagtat
    10921 caaagttaat ataagtgtaa gttctatttt taccttagaa aaaaggttta tgacattctc
    10981 taagaatttg actttagttt gtttattttt tttatacaat gtaaaacata gtatagtcaa
    11041 taaaaaactt gataatataa tacctatgca gacaaactct aattctactt ttgacaaatg
    11101 tacaggagta ccgaaaaaat aaaaataagg gagagcaaac tctctataat aataattaat
    11161 attagaaaag ttattattca aatcataaac tgcttcaaat aatatagaaa ctaatagaaa
    11221 cagtaataat aataaaataa attttgcttt agaatcatta ataattaagt ttttcaattt
    11281 tgtaaccaat gccccacacc acctttaaat attttggttc ttttggatta atttcaatct
    11341 tttctctaat tcttctaata tgaacagcaa tagttctctc acaactataa gcatcctcat
    11401 tccagacatt ttcataaatc tcatgagaag agaaaatttt accttttcgt gacataagaa
    11461 gtgtaagtat tttatattca agtggtgtca ttttaatttc ctcaccatct aaagaaacta
    11521 tttttgtatc tgtatttagc tctaggccac cactttttag aatataatta gtatcatttg
    11581 agttatttgt attactaaaa tcacctaaat ttatatacct tctaagttga gattttactc
    11641 gagcagtaag ttctagtgga ttaaatggtt tagttatata gtcatcagca cccatattaa
    11701 gacctataat tttgtccata tcttctgatt ttgcagataa gagaattatt ggtatattcc
    11761 ttttttctct tattttaagt gtagttttaa gcccatctaa attgggcatc attacatcca
    11821 ttattattaa tttaatatca ttttcgatta gtatgtccaa tgcttcaagt ccatcatatg
    11881 cctttaaaac attaaagtta tcaggtctca aatagaactc tatagcatca actatctctc
    11941 tatcatcatc tacaaccaat atattttgat tcaatttatt aacccccttg aaattttatt
    12001 tttaaccaat ttttatttta taccaaaaag aggtattaga aaacagtaag cccgttcatt
    12061 atgaaataaa aactagtact tttaatatat tgtcaatgca tatagtaaat ttaaaagtat
    12121 agacgcccat attaatctga aggtaaaagt ttatgaaaat atctagggta atctatcaca
    12181 cttcttatgt tattttggtt tattaatttt ttaggtttaa acatatatta acaaatataa
    12241 ataacaaatg agtttcataa ttattaagaa tttataacaa atatttattt taatgtagtt
    12301 taatataaac aggtagttta atgtaggtat gtataaagca tttaataagg cttataggtt
    12361 ttaatgatga tatctttata tattttaaga gatgatgaaa ttagatatgg gtaatagttg
    12421 tatgagaaaa agtagtatag cacaaaaagt gctatatcac aaaatctcaa ataatttcct
    12481 ttttataact taaattaaag aataaatatt tcttataaat taggtgtatg aagtatagca
    12541 ggaacatctt tattatttaa taacttatca aatgatttat ggtcagactt atagccaata
    12601 ccttctctat attctaattc atcaacaatt ttgtattgtt gacaggcatt tttaaccttt
    12661 tcatagtcct cagtagaatg agctttataa actatcttat tattaaaagt tattttatca
    12721 gcaaatttag gttttacaaa atatttagca tcttttattt taccagcatt atataaattt
    12781 tggataacag aaccattttt taaatgttgt aaatggtcat ctaatttata agtatgtgct
    12841 atatgagtta catttttaaa tttttcttca aatgtaagag caattttttc catgtaattg
    12901 aagtcagtat taccatttgt atttgtttct ggtaataata ttatattttc ccttattaca
    12961 ccaaggctat taagagaagc taaaaattct ctatttctat attctgattt ttgtatattt
    13021 gttaggtttt catatttttt atatttatca aagacggata taccacatcc agtagatact
    13081 aaaaccacaa atacatgatt ttttcctctt tcttttatag cattaacaat agcactccca
    13141 gcccacaaca cttcatcatc ttgatgctga acatagaaga caacataatc tttaaatttt
    13201 ttatgactat aattcacata gctactaaaa gcggttaaaa tcaaacataa aactagaata
    13261 atagatagaa ttttatattt atttatagta aattttttat tcttaaatgt catttaatca
    13321 tctcctgaaa ggttgtctta gttatattat caaacaaagt actaaaaatt atttcttaaa
    13381 tgctgaaaaa tatagagtag attaatggaa taaaattttt ttattagttt aaaataaaga
    13441 aaacatttat gtataagact aatcaaaata ttaatgattg ataggaattg agatttaaag
    13501 ggattgtaca tttattcggg attcatttta atagaatttt actttagtac ttagaataaa
    13561 agtaataact ttttaaattt aaagagtaca catatatatt tgtgtactcc ttgatgatat
    13621 gattgcttat ttactacaaa aacagagtct ttttaaacct aaaatatttg atgcaccagc
    13681 tatcgtagtt ccataatagt aaactggttt gttaagtgat agaaaattta taattgagtt
    13741 attacataat gtacttcctg tgcaaatcac taaatcagcc caagatatta catcttcata
    13801 atctcgtata ccatcttcaa tcttaacatt atattgaata gtatccacaa attcaggatt
    13861 taaatctaat actcttgttt cgaaaaagtc cttaatatta tcaattatgg ctggttgata
    13921 tcctataatt gctacttttg gattaccaaa ttccattttt ataaactcag ggaatttttt
    13981 tgcacatacc tctggctcgt tatttttaca atgaatggtt ctatccgtct tgcctaagta
    14041 tttcataact gcatttaatg atgctataaa aagacttcta tcatgagaat tgtcatgtaa
    14101 atctaatgat aatatgtctt ttaaagaacc ttcaaatgta gatggagtag aagtaaatgc
    14161 ttgtccttta gctcctttaa agtcagcttc aagcattatt tctttaccaa ctataattgg
    14221 gaaatcttta cgcttagtat ttccaattgc ttcttttgaa gatagagatt tggacacaat
    14281 acttatcata tccatattta aattatattt atcgacttgt actaatagat aatctctcaa
    14341 aatatcaaat atattcatag aaaaaagatt ctcctttatt ttttatttct tcatttaaat
    14401 caaaaatatg atttattttt ccattgttca ttattcctat cttatgagca aaaaacattg
    14461 cttcagaaat atcatgagta acatgaatca ctgttggttt gtattttatg tagacatcct
    14521 tcaaaatttt gcttatatgt ttttttgtaa tataatccaa tgaactcata ggttcatcta
    14581 aaagtaatat atcaggttca gctattaagg cacgtgcaaa agcaactcgt tgcttttcgc
    14641 ctccacttaa atttttagga tacctatttt ttatatcttc tatctgcatt aatttagaca
    14701 agtggttagc cttaagaatc tgttcatcta taggtatttt tctcaattta agtggaaacc
    14761 ttatattatc ttcaacattc atatttggaa ataattcaaa attttgatat acaaatccta
    14821 tatttctatt ttctggaggt aagtttgata ttaaattttc tttgtatgat acatttcctt
    14881 tagcagatgg atatcttcct gctaatgttt ctagaaatag agttttacct gacccactag
    14941 ctcctaataa aacaaagtat tcatgattat taatatttaa actattttct tgtaatttaa
    15001 aattaggtaa atcaagagat aaatttttta tttttaacat attaattctt atcaacctct
    15061 ctattaaaaa atatctcaaa aagcatcaag catgtaagtg agagaaatat caaaattaca
    15121 gctattccga tagcaatgtc tatatcacca gtagacatat ttaaatatat agatgtagga
    15181 ataatttcag ttttcatacg tgtagaacct gctatcatag ctactgcacc aaattcacca
    15241 agtgctcttg accaagtcat tacagttgca gatatgattc cagtttttag atttggaaga
    15301 attataaatc taaatgcttc ccaactatta catccaaggg tacgtgcaac aaatatcatc
    15361 ttttcattac tttcatttaa tgaagtgtga agtattttaa tagtaagtgg aagatttaca
    15421 aatatttgtg ctaaaactat accttgcttt gtaaatataa aatcaagctt aaaaatctta
    15481 taaatggaat ctccaatccc cattcttcca aacaaaagta aaagagcaat tcctgatact
    15541 aagtgtggaa gagacatagg gagatttatt atctgtataa tcagtttttt aaatggtaat
    15601 ttagtaatat gtaaaaaata tgctactgga atagctaata aaaggcatac tatagtagat
    15661 attaaagatg ttttaatact aagtgttatt gcatactgta cttccactga ttttaagcat
    15721 ataggaacat aacttatccc tttaatgatt aacataagaa gtggtgatac aataaacaaa
    15781 aagactatag caaaaaatcc agtaagtaga gtagtaaaaa atttattttt caagtttaat
    15841 cactacttaa caggttcaaa accatatttt ttaaatatct gtttagcttc gtctgactct
    15901 atataattta taaatttatt agctaaatct tttttatcac tatagtttac gattcctgct
    15961 gatatgagtt ggtctatatt tttatcttgg gcaatttcta taacttctac atcttcatta
    16021 ccaaagaccg aatcttttgt ggctattgta gcatcagctt tacttgatgt taactgtaca
    16081 atcatttcat ttacagttgc agctgtagag acaacattat catttataga ttttaaatta
    16141 tttttttcaa gtattttttg tgtagtctta cctatagcat tagactcttt gtctcccaaa
    16201 ataagtttaa ctccatcttt atttaagtct tccaattttt gaatattagc tggatttcct
    16261 ttaggtacaa ctattgcagg tgtatgatgt gcaaccgttt tatgttcacc tacaagttct
    16321 ttttcaaagg cctttgaata aattggttca gaaccaacaa taaatacgtc tcctttgtga
    16381 cttgtctcaa tttgagctat tagctgtgca gaacctgcat agctatattc aactttgaca
    16441 ttattttctt tctcaaattt ttttgctatt tcatccattg gtttctttaa acctgcccct
    16501 gaatagacaa aaagagtctc tttttctttt gaatcatcag ctgttgtcac tttagtatta
    16561 gattcatcat tagctttgtt ttctttagaa ttacatgcag taatacttaa tgaaataatc
    16621 atagctaaag ctagtactga aagtttttta aacataagat attccccctt attaaattag
    16681 tgtaaattct tttttagtat atcatactaa gaaaacgatg tctaagtttt tgttatgttt
    16741 tataatgttt tgttatgttt tgttatgtat agttaagaaa aaatctattc ataatgtcat
    16801 ttccataggt agtta
//
LOCUS       DAMJWU010000079        16741 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMJWU010000079 DAMJWU010000000
VERSION     DAMJWU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.081"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(59..277)
                     /locus_tag="PC460_002690"
     CDS             complement(59..277)
                     /locus_tag="PC460_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601568.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYESGSREPN
                     IETLILISNKLNVSTDFLLGLSPNQNRN"
     gene            396..584
                     /locus_tag="PC460_002691"
     CDS             396..584
                     /locus_tag="PC460_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601569.1"
                     /translation="MNNNLKLQREKVGLTQLEVAQKAKITERSYQYYEAGERLPNIRT
                     ALKIARILNTNCEKLFNE"
     gene            626..1420
                     /locus_tag="PC460_002692"
     CDS             626..1420
                     /locus_tag="PC460_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDF2601570.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEINHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSAYLDLKNENRKCYNVTKLGCDFLANKFTGEKGIIFTARYVKRF
                     NKMEQELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADS
                     VASSDNSILVGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLG
                     VIETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKKNNQISMLG"
     gene            1504..1884
                     /locus_tag="PC460_002693"
     CDS             1504..1884
                     /locus_tag="PC460_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601571.1"
                     /translation="MKNNTSDSRVKYFCKCPYCGFDNEVEVKKGSKPKICCICTKEVE
                     YEKLEQQSVLENTEIKEVCNQMKIKKLALATGEVFENIELKEFENKGECLPPNMVKVI
                     DGKQELLINKEFILSLEVKRKTTF"
     gene            complement(1874..2023)
                     /locus_tag="PC460_002694"
     CDS             complement(1874..2023)
                     /locus_tag="PC460_002694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601572.1"
                     /translation="MIPIAKNTKQTSKTVASKASKILKDGRYSAASKSVAGSALSQTK
                     KGPKK"
     gene            2085..2282
                     /locus_tag="PC460_002695"
     CDS             2085..2282
                     /locus_tag="PC460_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601573.1"
                     /translation="MAINDNINKILRDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKLADYLDVTLDELVGR"
     gene            2321..2527
                     /locus_tag="PC460_002696"
     CDS             2321..2527
                     /locus_tag="PC460_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601574.1"
                     /translation="MAKAVAKEQLFYRAKDIAKILDVCEATAYKIIGELNKELEKEGW
                     KTFPGRVSVTYFKERYCYKTKKGA"
     gene            2940..3257
                     /locus_tag="PC460_002697"
     CDS             2940..3257
                     /locus_tag="PC460_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601575.1"
                     /translation="MKSLIIVRNSVEQQLNRANLEINKNEQLYTKLRKKEERNISEEI
                     ELSNALREKSVNERLKIFAESLLKIIDTQIEIKEYEESEDYKIFELISEELERDIPID
                     VQI"
     gene            3340..3849
                     /locus_tag="PC460_002698"
     CDS             3340..3849
                     /locus_tag="PC460_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDF2601576.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRAKKNTLDKLKSNIKDCMELLETKKVETIL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            3859..4467
                     /locus_tag="PC460_002699"
     CDS             3859..4467
                     /locus_tag="PC460_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF2601577.1"
                     /translation="METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVVLDDNIVQIGNRFYVEATATLIDAETGEKISVKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNILYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            4477..5358
                     /locus_tag="PC460_002700"
     CDS             4477..5358
                     /locus_tag="PC460_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2601578.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLYKRSKNGRDATANILKELIENGYVTRKPCRDSNTNKMLGGYDYQVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYYESYIGVITPNNFLQLLSYLDDGMEADVIIRAVDEAVGSGVKNYKYIKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKSKQEKKQSNNKTVNTHNVSKNKFANFNQTFTQYD
                     EKELDEIIKKSQKEKFK"
     gene            5419..5592
                     /locus_tag="PC460_002701"
     CDS             5419..5592
                     /locus_tag="PC460_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDF2601579.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKIGREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            5608..6024
                     /gene="ssb"
                     /locus_tag="PC460_002702"
     CDS             5608..6024
                     /gene="ssb"
                     /locus_tag="PC460_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2601580.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKILFRVAVDRKYKDREGKK
                     KVDFIPCEQLGKHVENLVQYLTKGKQIYAFGELNIDNYKDENGCWKSITKVNVNALEL
                     LSSKEDSNNHEKQQEYIPPGLDPQGFQAIDDDDIPF"
     gene            6095..6508
                     /locus_tag="PC460_002703"
     CDS             6095..6508
                     /locus_tag="PC460_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601581.1"
                     /translation="MELKFREWNKNGKEMYSYDEMVCYSKNLLREWVYSGVYLPKNNE
                     NFEVMIYTGLKDCDGKEIYEGDIVLCRSIFFTEFAGEVKFKDGCFIAVNEMSGDCFRL
                     SELAIVKVDGNIYEDLSKLNDIRRCDEGGIRNDNN"
     gene            6495..6746
                     /locus_tag="PC460_002704"
     CDS             6495..6746
                     /locus_tag="PC460_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601582.1"
                     /translation="MIIIRSQDRLDLMRVNRVEIGFNQVYAILENDVRKIGEYESNER
                     AMQVLNEIQKFIENGVRTDYIDSCRVRHNQEKVFEMPFE"
     gene            6764..7117
                     /locus_tag="PC460_002705"
     CDS             6764..7117
                     /locus_tag="PC460_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601583.1"
                     /translation="MAKVWMDAGELLEKTIDIEDMFGCNLSKMRNKSKQKGLVGRTNS
                     KGQGRKSKKVECTNIITGESKIFVSAVEASKYLYFTELHIARLARTGKTTKNGWKVRY
                     IQEVTDGIGKCGASN"
     gene            7086..7226
                     /locus_tag="PC460_002706"
     CDS             7086..7226
                     /locus_tag="PC460_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601584.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDTAIKILSRYEYEGML
                     EK"
     gene            7342..7518
                     /locus_tag="PC460_002707"
     CDS             7342..7518
                     /locus_tag="PC460_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601585.1"
                     /translation="MDDRLEIINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
     gene            7628..8701
                     /locus_tag="PC460_002708"
     CDS             7628..8701
                     /locus_tag="PC460_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2601586.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSRKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGETMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
     gene            8781..8990
                     /locus_tag="PC460_002709"
     CDS             8781..8990
                     /locus_tag="PC460_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601587.1"
                     /translation="MYEYILRWQIGLSLENRKIHYTYGSKEALRKKAKALAKDENIVL
                     ITIDKVDEVIKNTISEKIIERFENL"
     gene            9002..10006
                     /locus_tag="PC460_002710"
     CDS             9002..10006
                     /locus_tag="PC460_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2601588.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVNQDMVLFFQKKISKVSR
                     DNDIRTAVFNNYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDKYHLRVLDKDAEKEEANSKFVTEFLNATKIKDDKYKTKKFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLREKHEIDINDFVDKTIKDDKLKDSFKEHMEEKGLVEGFSI
                     DKKWVDKKLKKRNIKTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIVIKNVTFYEEK
                     "
     gene            10088..10417
                     /locus_tag="PC460_002711"
     CDS             10088..10417
                     /locus_tag="PC460_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601589.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNDTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            10454..10825
                     /locus_tag="PC460_002712"
     CDS             10454..10825
                     /locus_tag="PC460_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601590.1"
                     /translation="MFNIYKVKIKTKRTLEQVRNQSVDFEYSEKGLKNTLKYYNLIDD
                     LKVIVVKFGDEYCLANYNEEDRKIIMEAHYLLEQDEYTGCYINEYERFKKDWENGNCD
                     GEACMVFSDDEIEIIEKLREG"
     gene            10828..11010
                     /locus_tag="PC460_002713"
     CDS             10828..11010
                     /locus_tag="PC460_002713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601591.1"
                     /translation="MNKRIKMKKRLIHKKCDERCVNYDFVISNNLITCNVCIGCKYKE
                     NMDKVCEENYKKLRSK"
     gene            11090..11431
                     /locus_tag="PC460_002714"
     CDS             11090..11431
                     /locus_tag="PC460_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HDF2601592.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNFKECESYLEGKNELYE
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELTK
                     INSHLELEDLR"
     gene            11557..11964
                     /locus_tag="PC460_002715"
     CDS             11557..11964
                     /locus_tag="PC460_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2601593.1"
                     /translation="MKVFLVIDGEPVGKERPRMNTYAKRTYTPNKTKNYEDLIKWLYQ
                     SKVKYYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVIKVVADSLN
                     EIAYKDDTQIVEVVASKYYSDNPRVEVILEDVI"
     gene            12066..12914
                     /locus_tag="PC460_002716"
     CDS             12066..12914
                     /locus_tag="PC460_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDF2601594.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHNIEGGFGEHKKAMLVKDIAEI
                     HNRESRQINELINKNRKRFKDGKDILDLLGVGLDDTKIKELGFTQQSINSYRGLKNKG
                     LLSGIYILSERGYAKLLKILEDDIAWELYEKLVDGYFSMRKELNNPLLSASKELQAIF
                     MLDKKQEVLETKIENVNEKLENFMDDAPLFNIECESIVKEVKKVATKSLGGHGSKAYK
                     NKSLRGKVYNDIYHQIKREFGVDSYKAIKRCQLNKVLEIVNNYKLPIVFEEEIRLLNS
                     QLSIVS"
     gene            13017..13505
                     /locus_tag="PC460_002717"
     CDS             13017..13505
                     /locus_tag="PC460_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601595.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSTVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKVMDMLYPRIKENELPLF
                     KN"
     gene            14252..14425
                     /locus_tag="PC460_002718"
     CDS             14252..14425
                     /locus_tag="PC460_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601596.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFGRF"
     gene            14425..15036
                     /locus_tag="PC460_002719"
     CDS             14425..15036
                     /locus_tag="PC460_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2601597.1"
                     /translation="MSGYLIVNLSNMLGELEEEEVKKILSSFSCPLNKDVEEFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIVGYFTLANKYFTIKRKTLSNSLARKIVKFGQYNE
                     ELRRYIIGAPLIGQIGKNYSNNYNKLIKGDELLKIACDKIKAVQLDMGGKIVYLECED
                     KPKLIEFYKDNGFVDFGKRSLDKDETDSLDGDYLVQMLKYLKK"
     gene            15113..15301
                     /locus_tag="PC460_002720"
     CDS             15113..15301
                     /locus_tag="PC460_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601598.1"
                     /translation="MEIKKNTQDVVSERKNSLDSEFKIPASGVCYMAEFIKESREIIK
                     ELDKHFESCLDVLSKARL"
     gene            complement(15281..15598)
                     /locus_tag="PC460_002721"
     CDS             complement(15281..15598)
                     /locus_tag="PC460_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601599.1"
                     /translation="MSKNIDIRNLKQFQKKIERMEQNLKPDFDKIRTIILSKKFMNQH
                     TNFDSFDELLAFGNYIVNSEEDFLAIPDNEFDLFIVKNTDFPDWQTMLDSAYSKYLES
                     CLR"
     gene            15722..15949
                     /locus_tag="PC460_002722"
     CDS             15722..15949
                     /locus_tag="PC460_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601600.1"
                     /translation="MGFEISGNLNFDSVIDDLKKEVENNPTIFTSQNVGNKFKEKCKI
                     CEKISEFEILEDGKVKCLECGTEFELNLKVE"
     gene            16019..16711
                     /locus_tag="PC460_002723"
     CDS             16019..16711
                     /locus_tag="PC460_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601601.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
BASE COUNT         7058 a         1642 c         3171 g         4870 t
ORIGIN      
        1 tagatggaaa tagagcaaac aacaatacgc ctgctaaaaa gcagacgaaa gttgaatatt
       61 aatttctatt ttggttaggc gaaagaccta acaaaaaatc tgtagataca ttaagtttgt
      121 tagaaattaa tattaaagtt tctatatttg gctctcttga acccgattca taatattgat
      181 atgcacgttc tgagatacca aacaaactag caaattgctt ttgagtcata ttcatttgct
      241 ttcttactag cttaatatta tctttgaatt tagtcataac tacacgctcc aataaaaaaa
      301 taaataaatt tcttgacacg aacaatatta gcgtgtaata tataaaacat agcacgaaca
      361 atattggcgt gttaaataaa aaggagaggg tgaaaataaa taacaattta aaactacaac
      421 gtgaaaaagt tggcttaacg caattagagg ttgctcaaaa agccaaaata acagagagaa
      481 gctatcaata ttatgaagct ggcgaacgtc tcccaaacat tcgtacagct ttaaagatag
      541 ctagaatttt aaatactaat tgtgaaaaac tctttaatga ataatagcat aaaaggactc
      601 agattacaag aaaggagctt tagatatgaa taatttgcaa cttaacaata aaaatacaat
      661 aacaacatta gaaattgctg atatgttaga aattaaccat tgggaagtat tgagaaaatt
      721 agaaggcaca gaaaaaacaa aaggaattat tgatattctt aacgacaaca attttgttgt
      781 ggttgattac tttataaaat cagcttattt agatttaaaa aatgaaaata gaaaatgtta
      841 caatgtaacg aaattaggct gtgacttttt agcaaataaa tttactggag aaaaaggaat
      901 tatttttaca gctagatatg taaaaagatt taataaaatg gagcaagagt taaaagaaca
      961 acaacctaaa ctaccaacta catacaaaga agcattacaa cagttattaa tagaagttga
     1021 agaaaaagaa caattacaat tagaaaatca agaaaaagat aaggtaattc agttacagca
     1081 accaaaagta ttatttgctg attcggtagc gtcttctgac aattcaatcc tagttggaga
     1141 attagcaaag ttgcttagac agaatggaat tgatacagga caaaatagat tatttgactg
     1201 gttaagaaat aatggttact taataaaacg taaaggtgag gattacaata caccaactca
     1261 aaaaagtgta gatttaggag ttatagaaac aaaagaaggt acaagagtac atccagatgg
     1321 tcatacaagt attactaaaa cacctaagat tactggtaag ggacaaatat actttattaa
     1381 taagtttaaa aagaacaatc aaatatcaat gttaggttaa agattaatag cactttgaaa
     1441 actaaataca gaatattttg aaatatattg ttttaattaa ttattaacta ggaggttaaa
     1501 tacatgaaaa ataatacaag cgattcaaga gtaaaatatt tttgcaagtg tccatattgt
     1561 gggtttgata atgaggtaga agttaaaaaa gggtcgaagc ctaaaatatg ttgtatatgt
     1621 acaaaagaag ttgagtatga aaaattggag caacaaagtg ttttggaaaa taccgaaatt
     1681 aaagaagtat gtaatcaaat gaaaattaaa aaattagcac tagcaacagg agaagtattt
     1741 gaaaatatag agctcaaaga atttgaaaat aaaggagaat gcttaccacc aaatatggta
     1801 aaggttattg atggtaagca agaacttcta ataaataaag agttcatatt atcattagaa
     1861 gtcaaaagaa agactacttt ttaggacctt tcttagtttg tgacaaagca ctaccagcaa
     1921 ccgatttgga agcagcacta tatcttccgt ctttaagaat cttactagcc ttagaagcaa
     1981 cagttttact tgtttgttta gtatttttag ctataggtat caccaccttt aaatgtattt
     2041 ataggattta tcctacaaat atagtatatc aaaggaggga aataatggca attaatgaca
     2101 acataaataa aattttaaga gatagagatt taaaagcgtg gaaattagca aaagaaatag
     2161 gcgtagattc aggaaattta tatgcaattt taagaggaga aaataaaaat ccaactatag
     2221 atacattgat aaaactagct gactatttag atgttacatt agacgaatta gttggaagat
     2281 agaaattaaa tacagaatat tttgaaagta ggtgtattgt atggcaaaag cagtagctaa
     2341 agaacaattg ttttataggg caaaagatat agctaagatt ttagatgtat gtgaagcaac
     2401 cgcttataaa ataattggag aattaaataa agaattagaa aaagaaggtt ggaagacctt
     2461 tcctggaagg gtatcagtaa cttatttcaa agaaagatat tgttataaaa caaaaaaagg
     2521 ggcataaaaa atgctatgag agtagctaaa gatatgtttc ctaaagtaat tgtataaaga
     2581 ggaagggggt gatttagttg aatgtaagag tactgatagc ttatatacag ttttgtaagc
     2641 aatacaataa gaaagcgagt tttgaaggtc ttaaaaaata caataagggg atagtgatat
     2701 aaaaaaactt aaagtaaaat ttcctacaaa aattcttaaa gcaggtaaag caatcagagt
     2761 aacatgtgca agatttggtt ttgaaagcga ttgtataata actcaatcgc aagaatttga
     2821 attaacagta gtttattttg ataaagaatt agatgattta gtacaatcgt ctataactat
     2881 agatgatgca ataagctatg attattgcat tgaaacgtta aattaagagg ggattaatca
     2941 tgaaaagttt aattatagtg agaaattcag tagagcaaca actaaataga gctaatttag
     3001 aaataaataa aaacgagcaa ctttatacaa aacttagaaa aaaagaagaa agaaatatat
     3061 cagaagaaat tgaattgagt aatgctttaa gagaaaaaag tgtaaacgaa agattaaaaa
     3121 tatttgctga gtcattacta aaaattatag atacacaaat tgaaataaaa gaatatgaag
     3181 aaagcgagga ttacaagata tttgaattaa tttcagaaga acttgaaaga gatataccta
     3241 tagatgttca gatataagaa aagagccact gcaatggctc taatcaaaaa tatatcaaaa
     3301 atttaattag ctatattata gcataaatgg agggaaatta tgagtacttt atacgaatta
     3361 actacagatt tattagaaat agaagaaggt ttaacagaaa caacaggaaa tgaagctgaa
     3421 aaactagagg aaataaaaga aataataaaa caagagatac aaaataaaaa cactaggata
     3481 gtttcagtaa tattaaacat tgacagtgat ataaactcta tagattcaga gattaaaaga
     3541 ttgcaagagt taaaaagggc caaaaagaat actcttgata aattaaaaag caatataaaa
     3601 gactgtatgg aattacttga gactaaaaaa gtagaaacaa ttttaggaaa tataagtata
     3661 agaaagtcgg caggtagctt agtcatagaa gatgaagaaa agatacctgc tatatataaa
     3721 acagttgagc aagttgtaaa agtagataag aacactatta aagactttat taaaaaaggt
     3781 catgaggttg aaggttgtag gattgaatat ggaactacac taacaattcc aaaagctaaa
     3841 aaagagtagg tgaggaccat ggaaactaat aatgtttata taaaacttgt aaatatacag
     3901 agtactttaa aagctcctaa aagtcaattt aatagctttg gtaaatacaa ctataggagt
     3961 tgtgaggata tactagaagg tttaaagcct attctaaaag aagaaaaagc attggttgta
     4021 ttggatgata atattgttca gataggaaat agattttatg tagaagctac agcaacttta
     4081 atagatgcag aaacaggaga aaaaatatct gtaaaagcat tagctagaga agatgaaact
     4141 aaaaaaggta tggatttagc acaagtaact ggaagtgtat caagttatgc aagaaagtat
     4201 gctttaaatg gattattttg tattgatgat acaaaagata gtgatgcaac aaataaacat
     4261 ggaaatgagc agaaaaaaaa agaagttaat gagagcgaat taaatatact atattcgcta
     4321 ggagaatcta tagaaaaaga taaaaataga gttgatagtg aagtatataa gaagtttgga
     4381 aagttagcag tagatttgac taagcaggag tatgagaaag ttttaaatgg atataagagc
     4441 attttagaga agcaaaaaca agagtaggtg atagtattgg ggattataag agtaagcaaa
     4501 gacaaagata atccatatgt agttttaaat aaaacttgtt tggaagatgt aaaattaagc
     4561 tggcaagcaa aaggcttaca ttcatatctg attagtaagc ccgaccattg gaaaatctat
     4621 gttaatgacc tatataaaag aagtaaaaat ggaagggatg ctacagcaaa tattttaaaa
     4681 gaacttatag aaaatggata tgtaacaaga aaaccttgta gagattctaa tactaataag
     4741 atgcttggag gatatgatta tcaagtatat gagataccac ttgaaaatcc tcagaagcta
     4801 aaatcccgaa aaactgattt cccggaaacc ggatttcccg gaaaccgggt ttctcggaaa
     4861 ccggaaaaca cggaagtagt aagtaatgac tttaaagtaa ataatgatat tactactatt
     4921 gttattaatg aacaatccaa taaagataaa accacctaca taaaaaaata ttatgaatct
     4981 tatataggtg tgattactcc aaataatttt cttcaactac taagttattt agatgatgga
     5041 atggaagctg atgtaataat aagagctgtt gatgaagctg taggcagtgg agttaagaat
     5101 tataaataca ttaaaacaat attaaataat tggatagaag caggtgtaaa aactaattta
     5161 gaacttacag agtatcaaaa tgagtttgag aggaagaaaa agagtaaaca ggagaagaaa
     5221 cagtctaata ataaaactgt gaatactcat aatgtaagta aaaataagtt tgctaacttc
     5281 aatcagactt ttactcagta tgatgaaaaa gagctagacg agattattaa aaagagtcag
     5341 aaggaaaaat ttaaataaaa attaaacttc taggaagtaa atatcaatat attgtttcct
     5401 agaaagggga ggtataaaat ggcgagaata tatgcacaaa gaagtggttc tttaaatgag
     5461 caggatagat tggaattgtt aagattactt gggaaagctg gatatacagt aaagattggt
     5521 agagagaagc aaaatagcaa gacaacttat acttactttg ttgagtatac agaagagcag
     5581 gaagaaaaat agaagggggc tagttaaatg aatacaataa ctttagttgg aagattagtt
     5641 gcagatgctg agctgaaata tcttccaaat tcgggtacac ctaaaatttt atttagggta
     5701 gcagtagata gaaaatacaa agatagagaa ggcaaaaaga aagttgattt tataccttgt
     5761 gaacagttag gaaaacatgt agaaaatctt gtacagtatc ttacaaaagg taaacagata
     5821 tatgcttttg gagaattaaa tatagataat tacaaagatg aaaatggttg ttggaaatct
     5881 attactaagg ttaatgtaaa tgctttagaa ttgctttcaa gtaaagagga tagtaataat
     5941 catgaaaaac aacaggaata tataccacca ggactggacc cacaaggttt tcaagcaata
     6001 gatgatgacg atataccttt ttaatcaagt taagtagtct agggagtaat tatacaatat
     6061 tacttcctag aagttaaaaa atggagggat gaagatggag ttaaaattta gagaatggaa
     6121 taaaaatggt aaagaaatgt atagttatga tgaaatggtg tgctattcta aaaatttgct
     6181 tagagagtgg gtttatagtg gtgtttattt gccaaaaaac aatgaaaatt ttgaggttat
     6241 gatatacaca ggtttaaagg attgtgatgg aaaagaaatc tatgaaggcg atattgtttt
     6301 atgtagaagt atatttttta cagagtttgc aggggaggta aaatttaaag atggttgttt
     6361 tattgcagtg aatgaaatgt caggagattg ctttagatta tctgaacttg caatagttaa
     6421 ggttgatggg aatatatatg aagatttaag taagttaaat gatataagaa gatgtgatga
     6481 ggggggcatt aggaatgata ataattagaa gtcaagatag attagattta atgagagtta
     6541 acagagttga aataggtttt aatcaagtgt atgcaatact tgaaaatgat gttagaaaaa
     6601 taggtgaata tgaaagtaat gaaagagcta tgcaagtgtt aaatgaaata cagaagttta
     6661 ttgaaaatgg agtaagaacg gattatatag attcttgcag agttagacat aatcaagaga
     6721 aagtatttga aatgccattt gaatagggag gggggaacta aatatggcta aagtttggat
     6781 ggatgcagga gaattgttag aaaaaactat tgatatagaa gatatgtttg gatgtaatct
     6841 aagtaaaatg agaaataaaa gtaaacaaaa aggtttagtt ggtagaacaa attctaaagg
     6901 tcaaggaaga aaaagtaaga aagtagagtg tactaacatt attactggag agagtaaaat
     6961 atttgtcagt gctgtcgaag caagtaaata tttatatttt acagaacttc atattgctcg
     7021 tctagcaaga acaggaaaga ctactaaaaa tggttggaaa gttagatata ttcaagaggt
     7081 gacagatggt attggcaaat gtggagcaag taattaaatt ggctgaaaag atattaaata
     7141 agaaaaaatg ttctgttaat aaagctattg atacagctat aaaaatatta agtagatatg
     7201 agtatgaggg gatgttagaa aaatgaatat attagctatt acaatgctta taataggaag
     7261 ttttatagct ggtagagttt atgagtatag attgaatcta aaagagtgtg aaaattgcga
     7321 caataaaaga ggtgtataag aatggatgat agattagaaa ttataaatgc ttctgtaaat
     7381 tatatacaga tgatatgtga aagttcaaat atagctatta tagcagagcg aggaagagtt
     7441 agaatattag atttagaaac taaagaaaaa tatgatttat taaaaaataa actcgaggaa
     7501 atgttagaag aaatataagt gaaaatatct aattaagaca gtttagagag ttacaaaata
     7561 tcttttagta taaattattg ttgaagtgtt ttgtgactct caaaaatgaa aataaggagg
     7621 cgttgtattg cttacatttt tagatttatt cgcagggata ggtggcttta ggctagggat
     7681 ggaaaaagca ggacataaat gtttgggaca ttgcgaatat gataaattcg caaatttaag
     7741 ttataatgcc atgcacaaac cgaaggagga tgaatggttt gaaagagata ttagagaaat
     7801 tagaacagaa aatatcccaa gagcagatgt ctggtgtttt ggattcccat gtcaagacat
     7861 ttctgttgca gggaaacaat ttggattcag aggagaacgt tcaagtttat tttttacagt
     7921 tacaaaactt attagagaac tcaaagaaga agatagaccc aagtatttac ttattgaaaa
     7981 cgttaaaaat ctacttagtg ttaatggagg atttgatttc ctcaaagttc tcgttgaact
     8041 ggatgaaatc ggctatgatg cagagtggca agttcttaat tctaaaaact tcggagtacc
     8101 ccaaaataga gaacgaatat tcattgttgg acattttaga ggacgaagta cacgaaaagt
     8161 atttcctatc gaaagaaaaa gtagaaaaaa tcttgagcaa ctaaataatc caactcatag
     8221 tacaaataga atttatgatg cagttggaat tgctagatgt attagaagtc aggcaggagg
     8281 tggaggtgct aaaacaggtc tatactttat agacttaaat aaaaactcta aagtaacaat
     8341 aaatgctaga tgccttaaag caaaatataa tgcaggtgtg acaaatagaa attgtgataa
     8401 tagtggagtt ttagttaatg cagttttaac gcccgatagg gtaaataaaa gacaaaatgg
     8461 tcgtagaatt aaagaaagcg gagaaacaat gttcacattg acagctcaag ataaacatgg
     8521 aattttgaaa aatggagata taagaaggtt aacaccaaag gaatgcttta ggttgcaagg
     8581 atttccggat aaatattacg aaagagcagc aagtgtatgc tcagatagtc aactgtacaa
     8641 gcaagcagga aatgctgtta ctgcaaatgt tgtatatgaa atagcaaaaa gaatgggcta
     8701 aaagttgcaa aatgtctttt agtatgaata tttttgaagt gttttgtaac tctcaaaaat
     8761 gaaaataagg ggtgggataa atgtatgaat atatattaag atggcaaata ggattatcgt
     8821 tagaaaatag aaaaatacat tatacatatg gaagtaaaga agctttaaga aagaaagcaa
     8881 aggcattggc taaagatgaa aatatagtac taataactat agataaggta gatgaagtta
     8941 taaaaaatac tataagcgag aagattatag aacgttttga aaatttataa ggggtggaat
     9001 tatgataata cacaaattta taatacatgt tttagataag aatagtgata caccaatatt
     9061 gaatgatttt gaaggtaggg ttaatcaaga tatggtccta ttttttcaaa agaaaataag
     9121 caaagtatca agagataatg acatcagaac agcagtattt aataactata gtaacaatct
     9181 aattaagaag tgttgtgaac aaattattta tgatgaaagt tcatttttaa ataactctaa
     9241 agagattgca gcttatttat ttgatgttat gaaattgaat gctacattag aatcttgcga
     9301 cttagcaatt tgtttatact ctcaaaaaga tgaaaagaaa gttgctatat taaagcttga
     9361 ttacaataat tcgtatactc attcaattga gtttaaagat gataaattta atatacagat
     9421 gtctaaaaat gaaattaata tacaagagac taagacggtt aaaattgctg ctttggttgg
     9481 attgagtgga atgaatgaca aatatcatct tagggtttta gacaaggatg cagagaagga
     9541 agaagctaat tctaagtttg ttacagagtt cttaaatgcc actaagataa aagatgataa
     9601 gtataagact aagaagttca aaaatacagc tgagaattgg ataactaatg ctcttagtaa
     9661 tgatataaaa caagcagagg atgtaagaag tatattaaat tatactttga gagaaaagca
     9721 tgaaattgat ataaatgatt ttgttgataa aacaattaaa gatgataagt taaaagatag
     9781 ttttaaagaa catatggaag aaaaaggtct tgttgaagga tttagtatag ataaaaaatg
     9841 ggttgataaa aagcttaaaa agagaaatat aaaaactgac aatggctttg aaataaaagg
     9901 taacttaact gattttgagg acccaatgaa atatacagta agacaaaatc aaaatgggtc
     9961 tatagatata gttattaaga atgtaacatt ttatgaggaa aagtaggtac tcatgtgact
    10021 attggctaga gaaggagaag taaataataa gaggatgtaa atttaaacta gttaggagga
    10081 ataacttatg aagatttttt tattgactat actgctaata attatttgta tattagcaaa
    10141 ttatgtgaaa aatcgcatat ataaaaaatc tataaataat ctaaaatata aatattctgt
    10201 aggggaaaag attatatatc atcaaataaa ctgttactat aacagaatgg ttggttgtga
    10261 aattttagaa aaatgttata gtacgaaatt tagaaaaaga aataccccgc tttataaagt
    10321 aaaagcatat gtaggtgata acgatacaac atgggttata ccagagtgga gaattgaatg
    10381 tcttgctacg acttatggag aatttcctaa atattaaata ataaaaattg gctggagaag
    10441 gagattgtaa attatgttta acatctataa agtgaaaata aagactaaaa gaacattgga
    10501 gcaggtaaga aatcaaagcg tagactttga gtattcagaa aaaggattaa aaaatactct
    10561 gaaatactat aacttgattg atgatttaaa agtaatagta gttaaatttg gagatgaata
    10621 ttgtctagct aattacaatg aagaagatag aaaaataata atggaagcac attatctttt
    10681 agagcaggat gaatatactg gatgttatat aaatgaatat gaacgattta aaaaagattg
    10741 ggaaaatggt aattgtgatg gggaagcctg tatggtattt tcagatgatg aaattgagat
    10801 aattgagaag ctaagggagg gttaaatatg aataaaagaa ttaaaatgaa aaaaagatta
    10861 attcataaaa agtgtgatga aagatgtgtc aactatgact ttgtaattag caataacctt
    10921 ataacttgta atgtgtgtat aggatgcaaa tacaaagaaa atatggataa agtatgtgaa
    10981 gagaactata agaaattaag aagtaaatag aataaaatag tcaaggtaag tttgtgaatg
    11041 aaactagaat gttatagact tactttgact tataaaagga gtgtgttaaa tggctaatat
    11101 atattgtgaa aattataatt gtaaaaacta ctttgaagat atgtgtatgc ttgaaagaat
    11161 tgaaattaat aacttcaaag aatgcgaaag ctatcttgaa ggtaaaaatg agctatatga
    11221 attagaaaat ggatatacta tacatcctaa agatttgaaa atggtgaaaa gtaaagatta
    11281 ttctgttgaa gttactcata ttccaactgg tattacagta aaatgccgtt ctacaaatag
    11341 tattttaaaa aataaaaata agtgtttgga agttctagaa gaagaactaa caaaaataaa
    11401 ctctcactta gagctagaag atttacgcta aataggaagt gagcttatga aacgaagaag
    11461 atgcagttgg tgtggtaagt tattttatct tgaagaaaaa tctaaggatg tttattgttg
    11521 taaagaatgt aggaagaagg ctaagaaggt gaaaaaatga aagtttttct tgtaatagat
    11581 ggagagccag ttggcaagga aagaccaaga atgaatactt acgctaaaag gacctataca
    11641 cctaataaga ctaagaatta tgaggaccta ataaaatggc tatatcaatc taaagttaaa
    11701 tattactttg aaggttatat aaaaatgact ttaagatgtt attattctat agctaaaagt
    11761 aacagtaaaa aggttaagga gcagaaaaga aataatgtgt taagacctag taaaaagcct
    11821 gacattgata atgtcattaa agtagtagct gattcactca atgagatagc ttataaggat
    11881 gatacacaga ttgttgaggt tgtagctagt aaatattata gcgataatcc cagggttgag
    11941 gttatattag aagatgttat ctaaccaacg gaaaaatccg ttcgttaaat tatgccctta
    12001 gtttttcata aaaatgtgaa aaagctaaat aaagaatata tcaaacgaca aaggagagat
    12061 aaattatgaa tgaaaatata aataaagaaa taacagtact tggaacttta gaaatcgagg
    12121 gaatgaaatt tcataacatt gagggtggat ttggagaaca taagaaagca atgctagtaa
    12181 aagatatagc cgagatacat aatagagaat ctagacagat taatgagctt ataaataaga
    12241 atagaaaaag atttaaagat ggaaaagata tattagattt gttaggtgtc ggtttggacg
    12301 ataccaaaat aaaagaatta ggatttactc aacaatcaat taattcttat agagggttaa
    12361 aaaacaaagg gttattatct gggatttata tattatctga aagaggttat gcaaaattat
    12421 taaaaatatt agaagatgat atagcttggg aattatatga gaagttagtt gatggatatt
    12481 tctctatgag aaaagaacta aataatcctc ttttaagtgc atcaaaggag ttacaggcta
    12541 tatttatgct agataagaaa caagaagtct tagaaactaa aatagagaat gttaatgaga
    12601 aattagagaa ctttatggat gatgcaccac tattcaatat tgaatgtgaa agtattgtaa
    12661 aagaagttaa gaaggtagca acaaaatcac taggaggtca tggaagtaag gcttataaaa
    12721 ataaatcttt aagaggtaaa gtatacaatg atatatacca tcagattaaa cgagagtttg
    12781 gagtagatag ttataaggct ataaagcgtt gtcaattaaa taaagtatta gagattgtaa
    12841 acaattataa gttacctata gtgtttgaag aagaaataag acttttaaat agtcaattat
    12901 caatagtaag ttaaatttat tcaaaaaaaa ggaaaaggag tgctttcaca ctccacttgt
    12961 caaaaatata aaacttttat ccaagattat tataacataa acaggagtgt ggaagtatgg
    13021 ataataatat caataaaaaa gaactattta aaaaagtaga aggtagatta catcattata
    13081 aatttttaag tgcagaaatt aagaatcttg aattagatat agaaagtaga gaaaatgaga
    13141 tatttgggtg taaggctgtt ggatatgatg aaaaagtaag tccaacatat gcttttaatt
    13201 caactgttga gaatgagatt ataaaaaaag aaagagatat tactagattg aaaaaactga
    13261 aaaaagataa agaaatagaa aagaagaaaa tagaaaatgc acttacatgc ttagatataa
    13321 gagaagaaca tttttttaaa ctgttttata atagtagaat gaaaaatagt atggtttata
    13381 tatccttaga gatgaactca gataggaaaa catgtagatg tgtaagggaa agattagtgt
    13441 ataaagttat ggatatgctt tatccaagaa ttaaggaaaa tgaactccca ttatttaaaa
    13501 attagaaaat tccccagttt ttccccagaa attcccactt tattccctac tttctcccct
    13561 ttttgattaa aaaagcatga gataatagta ttgtggaaat aaagatttcc ctctcaaaac
    13621 ttaatatttg actagggtat aagggattgc cctagtcact acgaacagac taggcagggc
    13681 gtgaggacgc tgttagttca attctaacta tgttcaaata ttaatcaacg tatacactaa
    13741 aagtagagaa attgagggca aaattttata ttttgtatct taattcagaa gtctaaaaat
    13801 cgggtggggc ttggtaacct cactcaccat gcaggtactg gtgtctagtc taagttcgat
    13861 tcttagaacc tgcgacataa tatatgtatc tccctactaa aaatgctaag tttactccaa
    13921 acttagcatt ttaattttta aaaagaaaaa agaaattttt attgtcataa tactatttgt
    13981 ttaggtatat tataatgtgc atacttaaaa ttaaatactt agcaagcatt tgaattaata
    14041 tacataagat atatgacata attttttata gtgtaagtta tttaaattaa ttataaataa
    14101 cagtaatttt attatataaa atgtacatat tgtgaataat aataataaaa tcatgtacaa
    14161 aatgccaact gataattcct cgaaatatat tgcatattta acgtaacgtc agtataatta
    14221 aattataata gtgaagtgga aggtggtact tatggctaca aaaagtattt taaaaaatgt
    14281 agatgtaaga aaaaaggcat ttggaagaaa tctagtatca gctctagaaa atgctaaaaa
    14341 taaacaagaa aaagaagttg tattaagtaa aaaatgttca gaagtaccaa aagataaaat
    14401 aaaggatata ttcgggagat tttaatgagt ggctatttaa ttgtaaactt aagtaatatg
    14461 ctaggagagc tggaggaaga agaagttaaa aaaattctct ccagtttttc ttgtcccctt
    14521 aataaagatg tagaagaatt tttaaaaaac aaagctattg aattttctaa acagggtttg
    14581 gctagtacac atttagtgct aacttcttat aaaggcaagc ctgttatagt tggatatttc
    14641 actctagcta ataagtattt tacaataaaa agaaaaacat tatcaaactc tttagctagg
    14701 aaaatagtga agtttggaca atacaatgaa gaactaagaa gatatattat tggagcacct
    14761 ttgatagggc aaataggaaa gaattattca aataattata ataaattaat caagggtgat
    14821 gaacttctaa aaattgcatg tgacaagata aaagcagtac agttagatat gggtgggaaa
    14881 atagtgtacc ttgaatgtga agataaacct aaattaattg aattttataa ggataatgga
    14941 tttgtagact ttggaaaaag aagccttgat aaagacgaaa cagattcgtt agatggggac
    15001 tatttagttc aaatgttgaa atatctaaaa aaataaaagt acataaaatc taaaatgact
    15061 atcttgatag atggtctttt tttatacaat aaattaaaag gagaatgaaa ttatggagat
    15121 taagaaaaac acacaagatg tagtatcaga aagaaaaaat tccctagatt ctgaattcaa
    15181 gatacctgca agtggtgtgt gttatatggc tgagtttata aaggaatcta gggaaatcat
    15241 aaaagaatta gataaacatt ttgaaagttg tctagatgtt ttatctaagg caagactcta
    15301 aatattttga atatgctgaa tcaagcatgg tttgccaatc tgggaaatca gtatttttaa
    15361 ctataaataa atcaaattca ttatcaggaa tagctaaaaa gtcttcttct gaattgacta
    15421 tgtaattacc aaatgcaagt agttcatcaa aagaatcaaa attagtgtgt tggttcataa
    15481 atttcttgct aagaattatt gtgcgaatct tatcaaagtc aggttttaaa ttttgttcca
    15541 ttctttcaat tttcttttga aattgcttta aatttctaat gtcaatattt ttactcataa
    15601 gaacacctcc tttcaataga atattagcat aaaattgtgg tgaattctgc tattgtcgaa
    15661 cgattgttga aggatattgt ataataacat agaatttact atactataag gaggtgatta
    15721 tgtgggattt gagataagtg gtaatttgaa ttttgacagt gttattgatg atttaaaaaa
    15781 agaagttgaa aataacccta ctatatttac atcacaaaat gttggaaata agttcaaaga
    15841 aaaatgtaaa atatgcgaaa aaatatctga atttgaaata ctagaagatg gtaaagttaa
    15901 atgtttagaa tgtgggactg aatttgaatt gaatcttaaa gtagagtaaa caaaaaaaga
    15961 atctcaatta tgaggttctt tttttattcc caaaacaaac aaataaagag gtggtgatgt
    16021 gcaagatgtc aaagaaaagg taaaacaaga ttacttaaaa ggaatgaaac aaaaggaaat
    16081 atcatcaaag tatgacatta gcttaaacac tttaaagtca tggataaaaa gatacaactg
    16141 ggctagtgaa aaaaagaagg gtgcacctat aaataaaaga ggtgcaccct tttctaataa
    16201 aaattcagtt ggtcatggtg ctccaaaaga gaataagaac gctgaaaagt ttggtttctt
    16261 ctcaaaatat ctacccgaag aaactaggga attgatacaa gaaatatcca taaaagataa
    16321 atttgatatt ctttgggagc agataacaat ccaatacgca gcaataataa gagcacaaaa
    16381 gataatgtat gttaaagaca aggaagaaat gattaaagaa ttaaagaaac atgaaagtac
    16441 agaaaatgga gagaagatag agtatgaatt tcaatttgca tgggataggc aagcatcttt
    16501 tcttaatgca cagagtagag ctatgagtga acttagaagt ttaattaaac agtatgatga
    16561 aatgattcat aaggattgga atttggctac agaggagcag aaaacaagag ttgagaagtt
    16621 gaaatgtgaa gttgataacc taagtaaaga tgatattgga gatgatgagt tgaaaataag
    16681 tgtagattat ggtgatagaa atgatagtta gagtaaattt taatccagat ttcaaggaag
    16741 c
//
LOCUS       DAMJWU010000080        16595 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMJWU010000080 DAMJWU010000000
VERSION     DAMJWU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.082"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            176..2035
                     /locus_tag="PC460_002724"
     CDS             176..2035
                     /locus_tag="PC460_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2601602.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2327..2926)
                     /locus_tag="PC460_002725"
     CDS             complement(2327..2926)
                     /locus_tag="PC460_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2601603.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3029..3826)
                     /locus_tag="PC460_002726"
     CDS             complement(3029..3826)
                     /locus_tag="PC460_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2601604.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3939..4044)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4136..5122)
                     /locus_tag="PC460_002727"
     CDS             complement(4136..5122)
                     /locus_tag="PC460_002727"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2601605.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5606..7336
                     /locus_tag="PC460_002728"
     CDS             5606..7336
                     /locus_tag="PC460_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF2601606.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7446..7526
                     /locus_tag="PC460_002729"
     tRNA            7446..7526
                     /locus_tag="PC460_002729"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7480..7482,aa:Leu,seq:tag)
     gene            7861..8376
                     /locus_tag="PC460_002730"
     CDS             7861..8376
                     /locus_tag="PC460_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HDF2601607.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8868..8972
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9182..11656
                     /gene="gcvPA"
                     /locus_tag="PC460_002731"
     CDS             9182..11656
                     /gene="gcvPA"
                     /locus_tag="PC460_002731"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF2601608.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11656..13113
                     /gene="gcvPB"
                     /locus_tag="PC460_002732"
     CDS             11656..13113
                     /gene="gcvPB"
                     /locus_tag="PC460_002732"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF2601609.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13536..15893
                     /locus_tag="PC460_002733"
     CDS             13536..15893
                     /locus_tag="PC460_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF2601610.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(15979..16296)
                     /locus_tag="PC460_002734"
     CDS             complement(15979..16296)
                     /locus_tag="PC460_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF2601611.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         6272 a         1923 c         2712 g         5688 t
ORIGIN      
        1 tatattagaa aaaattttgt atatatagag gactctaaag atgaactatg taaaacaagg
       61 tttatatatt ctaattgtat agaaaattta ttgtaaacgc atgtatatta ttagaaaaaa
      121 tagtttgaag gtattcaata aacataaaaa acaatatagg ggataaaggt gttttatgaa
      181 gagatttttg aggagaatta tcttagtttt atttattttg ttgattttta taagttttat
      241 ttctattaaa ttaattcata atgttggaga ttatggaaaa ttgattaatt atgttgggat
      301 tgttcgtgga gctagccaac gtctaactaa actagagatg aatcatcaac caaatgatga
      361 attaatagaa tatattgatg aaatcctaca ggagttgatt acagggcatg gagattatgg
      421 tttagtaatc actgattgta atgaatacaa tgaggactta cttttattag aaaaaaagtg
      481 ggaagattta aatattgaaa ttaaaaaagt aagaatgaaa gaacagaata accagttatt
      541 aagtataagt gaagagtttt tttctcttgc aaatgataca gtgtttaaaa tagaaaactt
      601 ttctaaagaa aaaagtaatt acttaatgac tttaataata attatatcta ttataggtat
      661 attagcatgt atcatattga ttttacaata tagtaaaaaa atggttagac tggaaaaatt
      721 aaatgtagat ttaaaaaata ttgcatataa agatgaacta acaggagtaa atactataga
      781 aaaatttaaa ttggatgcaa atcaaaatat ttgtatacat aaggataaga aatttgctgt
      841 attctatatt gattttgaaa actttaaata cattaatgat atatttggat atgattatgg
      901 tgacatgata ctgaaaagat atgctaatct tatgatgaat gacataggga aatatgaaat
      961 atttgcaaga gaaattgcag atagatttgt tgcgttaaga tgctatgtag ataaggaaga
     1021 tttagtggta agacaacgag atgtagacaa tgaactaatt aatataacaa atgaaattaa
     1081 aaataaacat agtattactg ttgttagtgg aatttgttgt attgaagatg taaatgaaaa
     1141 attaagtata gatggtttaa taaatagagc aaattttgca caaaaaactg ttaagaataa
     1201 accaggtact aattatgctt tttataatga cagtattcgt aaaaaaatga ttgaagaaaa
     1261 tactatcaag agtagaatac atgaagctat tgaaaaaaga gaatttatag tttatttaca
     1321 gcctaaagtt aatttacata gtcaaaaaat aaattgtgca gaagctttag ttcgttggct
     1381 tactccggat aaaggtatta tatcgccagc aatatttatt ccagtacttg agaagaattt
     1441 ttttattgcc ttagtagata agtatgtttt tgaggaggtt tgtaaatgga ttagaaagag
     1501 gttagatgaa aataaacctt ttgttcagat atcagttaat gtttcaagaa tacaatttta
     1561 taacactaaa tttgtagaaa cttattccaa tatacaaaat aaatacagga tacctaaaaa
     1621 tactatagaa attgaattta cagagagtgt tgcttttgag aatcaaaaac atcttttgga
     1681 aattatacat gatttgcatg aaaatggatt tacttgttct ttagatgatt ttggaaaagg
     1741 ttattcgtct ttaagtgttt taaaagattt accatttgat gttttaaagc ttgattctat
     1801 gtttttcaag gctagtttag ataaagataa agagaaaata gtaataaaaa atattgtaca
     1861 tatgttaaaa gaacttaata tcacaactgt tgctgagggc attgaatgca aggaacaagt
     1921 tgaatttctt agggatattg ggtgtgattt agttcaggga tttgtatttt ataaaccaat
     1981 gccaatatta gaatttgaaa aaatattgga taaagagttt gtttataata gctaaaatgt
     2041 actttataaa atatgaacta attaagtaaa attattttgt actgaataaa tttttacttt
     2101 atttacagaa taatgattat ttacagaaaa gcgcgaaaag tgtattcctt taagtgtggg
     2161 atggatagca caaatagagg agaaaaaata ttggccatat aagaatgcat aataatataa
     2221 atatttgaaa ggtcagcatt atataaaaat taaaaagaaa ataaataatt tttctataaa
     2281 aaagagacta tgagaaaatt tactcatagt ctcctaaata atgtatttat tttcttcctg
     2341 caacccattt taatccccaa tgaaattcag tatccatttc tctatgacct ttgaagtatt
     2401 taactacttt ttcaacactg aatgtctcat catttacatt ttctatcaat gctattgaac
     2461 acatattata gccattttta tgttcatcaa gtttgacaac taagtctgaa tcttgttttt
     2521 gtttgattgt tacaattcca tcagcttcag accagttagc tacaccttcg tatataaaag
     2581 cataaataag tattcttttt atatctttta ttttattacc atttattctt aaattttctc
     2641 cttgtgtatt acttcctgtt ctgtcatctc catctaattg agcaaaaggt ggattgtcta
     2701 aactaccaaa ggcatttcct aatgcttgaa cagctccttt tacaccattt ttcatctcaa
     2761 ataggcaacc caaatctaaa tctatattac tgcttcttaa agagccaaaa aaaccctttt
     2821 tttgtacttt ttgattccaa tttaaattaa ctaagatttc acctaaatcg ctattttcct
     2881 tttttgtaag gtttattttt tgtccctttt ttaattctat agccatagta atcttcctcc
     2941 acaaacatag atttatttaa atggtagcac ttaataagtc aatattcaac aagattagaa
     3001 taataatatt aaaattatac taaatatact aaaaagtagg aatcacaata cctttgtatt
     3061 tttcttcaat taagcttctt gctttatctg atgtaagaac atcttgtaga actttaatct
     3121 tttcactgtc tttatcttca gttctaaatg ctaaaagatt tgcatactcg tcatttatag
     3181 gagctgtata aattccgttt ttcttggcat caagaccagc aggtacagca taagtagtat
     3241 ctataaatgc agcagtcaca tctggaagag aagatggaat ttgagcaata tctaactctt
     3301 tgaattgtat tccctttgga ttttctatta tgtccttagg actaactaat ccatcccctt
     3361 taggtaacgt taaaagtcca atactttcaa ggtatctaag gttcttagaa caagaagatg
     3421 ggttattgct tattgcaatt gtatctcctt ttttaaattc atcaacagat tttatcttat
     3481 aagagtaaag tattgctggg catacatata gttttttacc agccattatt ttgtaacctt
     3541 tggccttaac agcttctttt agataaggtt catgttgaaa tagatttgca tcaatttcac
     3601 cattatttaa agcttcgttt ggaagtatgt aatcatcaaa gtttttaact tctaaagtat
     3661 atcctttttc tttaataagt ggttttaatt cttctaataa ctctcctcct ggaactaaag
     3721 tagcaccaac aacaattttt ttatcatctt tagctttaga acatccaacc acagctaaag
     3781 ttaatactag acataataac aaacataata attttttgaa tttcatataa attccctcct
     3841 gaatataatt gattagttaa aaataaaata ccattcttta tcacactgca aaataaaaat
     3901 aagcctgagt ttaaactcag gctttaaaag ctaatttaga ctcatctttc agtataatac
     3961 tgcaggattt agcaccaatg tatataaaaa tatactggtt gctgggtttc atagggccag
     4021 tcccttcacc actctggata agaagtatca atttttaatt atgttataaa tattatcatg
     4081 attttttacg tctgtaaaca taattttata aatattttaa aatctttaat gtattttatt
     4141 taacacctaa aattccagaa actagaatat cagtatcagc acctaagaaa tagtcattta
     4201 tttcaataga atccagagta tttctcaaag cattttcttc atgccttata ccaataagtt
     4261 tattttctac aaatgcaaca tcacattttc caaaaaaatc tccaaaaaat tttatactag
     4321 ttattatgcc tttatcaaca tttaaattaa actcaacatt tccaccagaa tattttaatt
     4381 catttgaaag agtatatttt ggtgaattac caaaattcca atcccaagtt tcgtattttg
     4441 atttagtaag cttttctatt tgttttatat catgctcact caaacaataa ttagtattat
     4501 cagtatttgt tttagaaaga taatccatta tagcatcttt aaattctaat atgtccattt
     4561 ttgaatgtaa atgttcactt atattggtta cacgagattt tacagatttt attccttttc
     4621 cttcaaattt tataggttta acctttaatg cttttgagag gtcatttatt tcagatgaaa
     4681 ataaaagagt accatgatgc attactttgt ttttgtagtt gtattgagca tttccagaaa
     4741 atttcttgcc atctataaga aggtcatttc ttccagaaaa ttcggcattg acatctagtg
     4801 ttttaagtaa atctattatg ggctgagtga atcttctgaa atcactaaaa ttattattgt
     4861 tacaagctat aaatgtaaaa tttatatttc ccaaatcatg aaatacagct ccaccaccag
     4921 actgtctcct aacgacaggt atagaatatt cttttatata ttctaaattt atttcagaaa
     4981 gtgtgttttg gttttttcca acaattatag aaggttcatt cctccataaa ataaataagt
     5041 cttcatcaaa attttttaga agatattctt ccattgctaa attaaaataa gggtttgttt
     5101 tttcattata aattaataac attaaagtag ttcctccata gaattgataa aatttttatt
     5161 atgaatacaa ttaattttat aattcgacaa taatataaaa actcctttaa attagaaatt
     5221 ttaaaaacta ccttgcaaag tttaaataaa taatatatac tcaaaatatg tttaataaaa
     5281 gtgaatagaa gggtagaggc gcaataatct atcagtacta ataaggagtt aagcacagag
     5341 atttattagg aaaggagata ttgccgaagt tttataatcg atgctttaag attatattgc
     5401 tgggggtaca tagaaaatat gtacaactgt cacaggttta tttgtggtga gctatcattt
     5461 aaggaattat attatattta tttttactat tgttgatata ttgtaatgtt taagccttga
     5521 gtgtttacca ctcaaggctt ttttagtttt attgtaacat aataccctgt acagttcaca
     5581 aaattaaaag gagagggaga tttatatgag gaacaaaaaa attaatatta tttttctgac
     5641 gacaatcatg ttcataatgt caacagtaat ggtttttgca gaagaagata tagacactat
     5701 tgcactagcc aatgcagaaa aatttgggat tttaacttta ataccaccat tagttgctat
     5761 aatacttgca tttataacta aaaacgtaat aatttcttta cttataggta ttctttctgg
     5821 tagttttata attaaagcta gtggtattaa tgtttttgcg acatttatac aggcattttt
     5881 agatttagta gacagagctt tagtatcttt agcagaccct tggaatgcag ggataatact
     5941 acaggttttg gcaattggag gagtaataaa tcttgttgct aaaatgggag gagctaaagc
     6001 tatagctgaa gcacttgcta aaagagcaaa gtcagcaaaa gggacacagc ttataacatg
     6061 gtttttgggg cttttagtat tctttgatga ttatgccaat tcactaatag taggtcctat
     6121 gatgagacct gttgcagata aaatgaaaat atcgagagaa aagctagcat ttataataga
     6181 tgccacagca gcaccagttg ctggtttagc tataatatct acatggatag gtcttgaggt
     6241 tggtttaata catgatgctt ttgaaagtat aagtatagat gtagatgcat ttggtatatt
     6301 tttaaataca ataccattta gattttacaa tatattaata ttagctttca ttgtaatatc
     6361 agcattatta ttaaaagaat ttggtccaat gagaaaagct gagataaaat ctagaagtag
     6421 aaaaattagt atagaccttg atgaaggcgt agaagagttg gatgatttag ctccaaaaaa
     6481 tggagtaaaa ttgagtgtat ggaatgctat aatcccaata ggaacattga taatagttgc
     6541 attagcatca ttttattata gtggatatac atctataatg ggaggagacg ataaggctct
     6601 gatacaacta tttacaaatt ctccatattc ttttgaagca ataaaagaag catttagtgc
     6661 atctgatgca tctagagcat tattccagtc tgcacttgtt gcaagtttag ttgctattat
     6721 aatggctgta gtaaaaaaga tttttacaat atcagaagca attgatgttt ggatagatgg
     6781 aatgaaaagt ttagtaatca ctggtgtaat attaatactt gcatggtcat taagttcagt
     6841 aataaaagag ttaggtacag ctaaattttt aatacaccta ttatcaggtt cactaccacc
     6901 attcttatta ccaagtttaa tatttggttt aggagcaata atatcttttg ctactggaac
     6961 agcttatgga acaatgggta tacttatgcc acttgcaatt ccattagcat actcattaaa
     7021 tcctgatatg tcttatgtaa tagtaagtac tagtgcagtt ttaacaggtg caatatttgg
     7081 agaccattgt tctccaattt cagatacaac aatactttct tcaatgggag ctggctgtaa
     7141 tcatatagac catgttaaca ctcaaatgcc atatgctata tttacagcag taatcactat
     7201 tgtatttggg tatataccag ctggacttgg attgccaata tatatagtat taccagtggc
     7261 aatagctgct atatttgtag gtattcaaat aattggtaag aaagttgatg aagcggaaat
     7321 agaacttgta gaataaagat ataaataagt ttcacctttt attatcacta ttttgttagt
     7381 ttttagataa ttttaaaaag ttattgaaaa taatggcgaa atatagtaaa ataaaagagt
     7441 gataagcgga tgtggcggaa ttggcagacg cactagactt aggatctagc gccattggcg
     7501 tgggggttcg actcccttca tccgcacttt acagtactta attgtactga aaaaaactgg
     7561 ggttgctggc tttcggagtg ggctatcccg tttttatttt tttgtatata taatagattg
     7621 gcactttagt ataaggtgct ttttttatag gcaaaaacag tacatttatt tctgttttat
     7681 tatcattcat ctattagaat atactttagt taagaatgtg ttaataagca gtgttcacat
     7741 tcttgataaa atgtggaata atatgtattt ttactaagac ggaaatgaaa taattaaacc
     7801 attaaataat attataagat gatgaataag tagataaatc aaattttgta ggaggatatt
     7861 atggaaaaat atatatggtt gtttccatta ctttttattt ttcatgatat ggaagagata
     7921 ataggttttg gaatatggct taaaaaaaat aaatcaatgc ttgataagaa gtatccattt
     7981 atcagtaaag tttatgaaaa ttatagtact gaaggcatgg catttgctgt gtttgaagaa
     8041 tttattttat gcattatttt ctgtatactt acagttataa cagagaatca gtatgtatat
     8101 ctactatggt taggtagttt tatagcatat accttacatt tggtaattca tatagggcag
     8161 tcaatcataa taagaaaata tataccttca cttattacca gtatcatatg cttaccaata
     8221 agcatttggt gtataagtaa gagtatttat atagttgatt gtgaaatgag tacaacaatt
     8281 ctgtacagta taattggcat tataattgta gcattgaatt taaagtttgc acagtcgctg
     8341 attggaaaat ttacaaagtg gatgtcaaac atataaattt tagtttattg gacagttcaa
     8401 ggaatcagaa attggaattt tatagtatta atattttatg gtattaatat aatgtataaa
     8461 attttatagc aatgtatttc ataagtataa agtgactaga attttaagaa atatacaaat
     8521 tctataaaaa ctaaataatt tcaagtatat tttaaaaatc ttatttttca caagtttaaa
     8581 attgtaaacg atttcatcaa gtctatgaat atacttttaa catgttatag taaatctcaa
     8641 tataaattta catttggttc tttgtgtgtt attttaataa taaattaagt gaaaatgtta
     8701 ttttacatta atatataata tctttgcata agagtgaata atataaaaga gaaataattg
     8761 ataataaaaa cttaacaaaa aaattaatac ataaaaaaaa ataaatataa tgtgttgaca
     8821 tttaattgaa atattcatac aataaaattg aaaataaata tttccaaatg agattagcga
     8881 gagagtcctt tatacgaaaa ggcgccgaag aagaaattta agaaatgcca atttttagag
     8941 aagctttcag gcaaaaatat cgctaattga tgggactctg gagagactta agttaagata
     9001 ctataagtca ccgaagaagc aaaataatat taaattatta aatctttcag gtatacaaac
     9061 agggatatat aggctaggat tattttccat gctgatatat ctttttttta tgaaaaagta
     9121 caggaaataa tatcaaagtt acttaaatac agtaggtcta attttctagg gagggttata
     9181 tatgaatgaa ttgaaaagag tatctttgta caatatacac aaagagcttg gagcaaaact
     9241 tgttgaattt gctggttggg aaatgccttt agaatatgag ggtataaata aagagcatga
     9301 gaaggtcaga aaaagtgcag gtatatttga tgtatctcat atgggagagg tacagataaa
     9361 gggagcagag tcagaaaaat ttatacaaaa cctagttaca aatgatataa gcgcattaaa
     9421 gataaatgat attatatata cacctatgtg ttatgaaaat ggtggagtag ttgatgattt
     9481 attaatatat aaatttggtg aagaagatta tttattagta ataaatgcag gtaatataga
     9541 taaagatgta gcatggataa taaaacaaag tgaaggatac aatgtagata taaaaaatat
     9601 atctagtgaa gtgagtcaat tagcaattca aggacctaaa gcagaagaaa ttcttcaaaa
     9661 aatcacagat atagacttaa actcaataaa attttataag tcaattccat caacaaaagt
     9721 atgtggttgt ccttgtttag tttcaagaac aggatatact ggggaagatg ggtttgagat
     9781 atactgtaaa aataaatatg tagaaataat ttggaatgaa gttctaaagg ttggaggaga
     9841 agatatatgt ccagctggtc taggttgtag ggatactttg agatttgaag cagctctacc
     9901 tctatatgga catgaaataa atgaacacat atctccaatt gaaggtggtt tatctatctt
     9961 tgtgaaaaca aataaagaat catttattgg caaatcaatt ttatcaaaag aaaaagaaag
    10021 tggagctaaa agaaagttag taggatttga aatgcaaggt aaaggaatgc ctagaaatgg
    10081 atatgatata aggattggtg ataaaacagt tggatttgta acaacaggat gtgcatctcc
    10141 aacgacagga aaaattttag gtatgggaat tatagattct gaatatgcaa aagtaggaaa
    10201 tgaaataggt atagcaatca gaaaaaaagt tgttcctgct gtgatagtta agaaaccttt
    10261 ttataaaaaa caatataaaa aagataatat tatattaaac aaagaaaata aattttcata
    10321 tatacctgct acaagtgaag ataaatcaaa aatgttaaaa gtagttggat taaattcggt
    10381 tgatgaatta ttttcagata taccagaaga agtaaaatta aaaagagatt taaatttgga
    10441 aattggaaaa tctgaattag aggtaagcaa aatagtaaaa agattgtcag aagaaaattt
    10501 aagtttagaa gacttgactt gttttcttgg tgctggtgca tatgaccatt atataccatc
    10561 aattataaaa catataactt caagatctga attttataca gcatatactc catatcaagc
    10621 tgaaataagt cagggaactt tacaagtagt ttttgaattt caatctatga ttgcagaaat
    10681 tacaggtatg gaaattgcaa atgcatcaat gtatgatgga gcaactgctg caatagaagc
    10741 atgtataatg gcaatgaatc agactagaaa aagtaaaata gtagtgtcaa aaactattca
    10801 tcctgaaact ttgtcaatat taagaactta cttacaatat aaagattgtg agatagtaga
    10861 aattgacttt tgtaatgaat atggcactac agatatagaa aaattaaaag cttctgtaga
    10921 caaagataca gcatgtgttc ttatacaaac tcctaatttc tttggaatta ttgaagaaat
    10981 ggaagaaata gaaaagataa ctcatgaaaa taaggctatg ttaattatga gtgtagaccc
    11041 aatatcatta ggagttttaa aaacaccagg tgagataggt gcagatattg ttgttggaga
    11101 agctcaatca cttggaaacc ctcttaattt tggaggtcct tatgtaggat ttttagctag
    11161 caaatctaaa tatactagaa aaatgccagg aagaatagtt gggcaaagtt tagatgtaga
    11221 aggtaaaatt gcatatgtat taactttaca aactagagaa cagcatgtaa gaagagaaaa
    11281 agcaacttct aatatatgtt caaatcaagc tttaaatgct cttgtagctt caatatatat
    11341 agctactatg ggtaaagaag gatttaaaga ggttggtatg cagtctatga aaaaagcaca
    11401 ttatacttac aataagcttg tacaaactgg aaaatataag ccaatattta aaggtaagtt
    11461 tttcaaagaa tttgctgtac aaggaaatct taatatagaa actataaatg ataaactctt
    11521 agaagaaaat atattaggtg gttataattt agaatacaat tatccagaat taaaaaattc
    11581 aactttgctt tgtgtgactg agaaaagaag caaagaggaa atagataagt tagttggaat
    11641 aatggaggga ttataatgaa agaatacaat agtttgttga tagatatatc taaaaaaggt
    11701 aggaaagcat actcacttcc aaagttagat atagatgata taaaaataga aaatatgatt
    11761 gaccaagata tggcaagaca aagtgagttg aatttgccag aagttggtga attagaatta
    11821 gttagacatt atacattatt atcaaataaa aactttggag ttgatacagg tttttatcct
    11881 ttgggttctt gtactatgaa atataatcct aaaataaatg aagatatggc agcacttcca
    11941 aactttactg gaatgcatcc ataccaatct tcggatacag ctcaaggttc tctttctttg
    12001 atgtatgatt tatcaagaag acttgcagaa attacaggta tggatgaagt tacactacaa
    12061 ccttcagcag gttctcatgg tgagtttaca gggcttatga ttataaaagc atatcatgaa
    12121 aatagaggtg accataagag aaccaaagtg ataattccag attctgcaca tggaacaaat
    12181 ccagcaagtg cagctatggc aaactttgat gtgattcaaa tagcatctga taaaaatgga
    12241 gctgtagaca taaatgcatt aaaagaggtt ttgaatgatg aagtagcagc tcttatgctt
    12301 actaatccaa gtactcttgg tttatttgaa aaaaatataa aagaaatagc tactcttgta
    12361 catgaagcag gaggactttt gtactatgat ggtgcaaaca tgaacgctat tatggggata
    12421 acaagacctg gagatatggg gtttgatgtg gttcatctta atcttcataa gactttttca
    12481 actcctcatg gaggtggagg tccaggagct ggtcctgttg gagttaaaaa agaattagta
    12541 ccttttttac caataccagt aattgaaaga aaagaagata agtatgtact aaattatgat
    12601 agagaaaaat ctattgggaa aattaaaaat ttctatggta attttggagt tcttgtaaga
    12661 gcttacacat atatactaac tatgggaagg gatggactta aagaggctag tgaaatggca
    12721 gttttaaatg ctaattatat aaaggaaagt ataaaagatg attatatttt gccaatagat
    12781 accttgtgta agcatgagtt tgtattaggt ggattgccaa gaggagaggc caatataaaa
    12841 actatcgata ttgcaaaaag actactagat tatggatacc atcctccaac aatgtacttt
    12901 cctcttatta taaatgaggc acttatgata gaacctacag aaagtgagag tatagagaca
    12961 ttagatagtt ttatagaagc tatgaaaagt gttgctaaag aggcaaagga agaaccagaa
    13021 ctattgaaaa ctgctccaca taatactttg gtcaaaagag tagatgatgc aagggcagta
    13081 aagaaaccta tactaacatg gtcacaaaga taaatagtta aaaagtagag atttaaagta
    13141 taaagagaat taaatttgta agaaaaagct atctaactta atctagttta gatagctttt
    13201 ttgtatatta gtatgtatgg tattttctat ataaagtttt aaattatatt agtgcttata
    13261 tagaatattg atatgatgat tatgtataga tattaataag aattgttgaa tttatcttgc
    13321 cttaacttta tttactcttt tatattaaga aaaacttaac taaaatatag cgaaattcat
    13381 aaaaatttag gaaatcttaa gaattataat atttaaatct ttagatttat attttattat
    13441 ataaactata gaataataaa ataaaaaaat tgagaatttt ataatatatt tgtagatttg
    13501 agtataaaaa agtgctaagt tgaaaggagt aaaatatgga taaggttaat ttagaaaggt
    13561 atagttgtga tataaatgtt ggtttaaata aaaatcaact tcaaagtaga atagatgata
    13621 atttaattaa tgcatttgat aatggaaaaa ccaaaacata taaacaaatt tttaaagaca
    13681 atatatttac actttttaat ctcataaata ttgttttagt ctgtttatta gtttcagttg
    13741 gttcttttaa aaatacctta tttatattta ttgctgtaat taatactatt ataggagtaa
    13801 tacaagagat aagagctaaa agattattgg acaatctatc tgttatagtt tcaaataagg
    13861 tatctgtcat aagagaaaaa gagaaaaaag agattgatgt acaagaatta gtattagatg
    13921 atattatgat tttaaaaaca ggtaatcaaa tttgtgcaga ttcaaaagtt ttaaatggaa
    13981 aattagaggt aaatgagtct ttggttacag gtgaatctga tattattata aaaaataaag
    14041 gcgatttttt atattctgga agttttgtag tttctggaga agcttttgta agagttgaaa
    14101 atataggaaa agataactat gcaaataaga tagctaatga tgcaaagatt tctaaaaaac
    14161 ataattctca acttagaaat tcattaaatg caatattaaa aattgtaggt ataataataa
    14221 ttcctttagg aattatattg tttgcaaaac aacattttgg aaatggagat tctatagcta
    14281 catctgtagt aggtacagtg gcagctgtaa atggtatgat accagaggga ttaacattac
    14341 ttacgagcat tgctctagct gtaggaacta taaaacttgc tagtcataaa actttagtgc
    14401 aagaactgta ctgtgtagag actcttgcta gagttgatac actatgtctt gataagacag
    14461 gtacaattac agaaggtaaa atgcaagttg atgatatagt aatgttggaa gaagtggata
    14521 ttaatgaaat aatggggaat atatgtaatt ctctcaaaga tgataatgca acactaaatg
    14581 caataagaga taagtataat gttttagata cgtataaagc taagaattta ataccatttt
    14641 cttcagaaag aaaatacagt ggggttacct ttgaaaataa aggtacatat ttattaggtg
    14701 catttgagtt tatatttaag gaaaaaaata aaaaacttag gatagaagtt gaaagatatt
    14761 ctaaaaaagg aaacagagta atagttttag ctcatagtga ttcttatatg gaagataata
    14821 atattcctga agatataaag ccactagctt ttatattgat attggataaa attagagatg
    14881 aagctaagga aactttagaa tttttttaca atgaaggtgt agatataaaa attatatctg
    14941 gtgacaatcc tataacagta agtgaggtag ctagaaaagc aggtcttaaa acagctagta
    15001 attttattga tgcaacaaca cttaaaaatg atagtgatat atacaaagca gttgataaat
    15061 acagtgtttt tggaagggta agcccacaac agaaaaaaca aatgatttta gcattaaaaa
    15121 aacaaaatca tacagtagct atgactggag atggtgtaaa tgatgtacta gcacttaaag
    15181 aagcggattg tagcattgcc atggcatctg gaagtgatgt tactaagaat gtgtctaatt
    15241 tagtcttatt agattcaaac tttgcatcaa tgccaaaggt tgttatggag ggaagaaaag
    15301 taattaataa cattcaaaga gcatcttctt tgtttttagt aaagacaatt ttttcattct
    15361 tcttgtcgtt attaacatta ttcttcttca gtagacagta tccatttgtt cctatacaat
    15421 tatcactcat tggggctgta acagtgggta taccatcatt cttcttagct cttgaagcaa
    15481 acaaaagtag aatttcagga aattttttac tcaatatatt aaaaaatgct ttaccaggag
    15541 cactatgtgt gacagtaaat gtaattattg tttacaatat agttgaatat ttaggttata
    15601 gttcaagtgt attttccaca atgtgtgcta tattaactgg tgtatgtggt cttttaatat
    15661 tgagacaaca atgtttgcca tttaataaag aaagattatt tttaatagta agtatgacta
    15721 ttgcatttgt tattgggatt ctatttttag gtggattatt ctcatttgta gcattgagta
    15781 gagaattaat tctcataact atggcattag caatattaat gccaattatg ataaaagtta
    15841 tgttatttgt cttagatgag atattagaga caattgttcc agatttaaat taattttaaa
    15901 atgatataaa taagagcagt tttagttgaa ctgctctttt tagatgtgtt taattttatg
    15961 taagaatatg tatttttatt aatcagctat atatgagcaa cagtagtcaa caacttcatt
    16021 aacctttaag tcaaaacttg aatcactagg aacattaaat ttttgccctt ctttataagt
    16081 tacaaactca ttgcttccag gcagttttac atccatactt ccagcaagca tctccattat
    16141 ttcttcttct ctagtagaaa aagtgtattc tcctggcaac attaatccta aagtttttct
    16201 ttctccatta ggcagtatta ttactctgct acttacctta ccatcaaaat aaacgtttgc
    16261 cttctttact gcagtaacat tcttaaattc actcattaga taaaacctcc aaaattattt
    16321 gtacatatat attatataaa aataatgtaa aaaatgctat atatttacaa aaaaatttag
    16381 tatttattaa aaaaattaat aagaataaaa aagaggtgaa attgcttaaa ttgataaaaa
    16441 atataaaata aattgcaaag taagcaagaa aagaataagt tgaagagtta tatattataa
    16501 atattaaaaa ttttagtatt aataaaaaag atattacatt aatatgaaaa tataggagat
    16561 tagaagtgtt tgattaaaaa atagctacac acact
//
LOCUS       DAMJWU010000081        16350 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMJWU010000081 DAMJWU010000000
VERSION     DAMJWU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.083"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..369
                     /gene="tuf"
                     /locus_tag="PC460_002735"
     CDS             <1..369
                     /gene="tuf"
                     /locus_tag="PC460_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2601612.1"
                     /translation="DNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEE
                     GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLR
                     FSIREGGRTVASGVVATIIE"
     gene            619..768
                     /gene="rpmG"
                     /locus_tag="PC460_002736"
     CDS             619..768
                     /gene="rpmG"
                     /locus_tag="PC460_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF2601613.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            794..1015
                     /gene="secE"
                     /locus_tag="PC460_002737"
     CDS             794..1015
                     /gene="secE"
                     /locus_tag="PC460_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF2601614.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            1035..1577
                     /gene="nusG"
                     /locus_tag="PC460_002738"
     CDS             1035..1577
                     /gene="nusG"
                     /locus_tag="PC460_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF2601615.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1616..2041
                     /gene="rplK"
                     /locus_tag="PC460_002739"
     CDS             1616..2041
                     /gene="rplK"
                     /locus_tag="PC460_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF2601616.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            2112..2810
                     /gene="rplA"
                     /locus_tag="PC460_002740"
     CDS             2112..2810
                     /gene="rplA"
                     /locus_tag="PC460_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF2601617.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2862..2990
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3033..3539
                     /locus_tag="PC460_002741"
     CDS             3033..3539
                     /locus_tag="PC460_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF2601618.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3597..3962
                     /gene="rplL"
                     /locus_tag="PC460_002742"
     CDS             3597..3962
                     /gene="rplL"
                     /locus_tag="PC460_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2601619.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            4354..5142
                     /locus_tag="PC460_002743"
     CDS             4354..5142
                     /locus_tag="PC460_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2601620.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5613..9329
                     /locus_tag="PC460_002744"
     CDS             5613..9329
                     /locus_tag="PC460_002744"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF2601621.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIVKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9371..12856
                     /gene="rpoC"
                     /locus_tag="PC460_002745"
     CDS             9371..12856
                     /gene="rpoC"
                     /locus_tag="PC460_002745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF2601622.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            13145..13567
                     /gene="rpsL"
                     /locus_tag="PC460_002746"
     CDS             13145..13567
                     /gene="rpsL"
                     /locus_tag="PC460_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF2601623.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13691..14161
                     /gene="rpsG"
                     /locus_tag="PC460_002747"
     CDS             13691..14161
                     /gene="rpsG"
                     /locus_tag="PC460_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF2601624.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            14210..16276
                     /gene="fusA"
                     /locus_tag="PC460_002748"
     CDS             14210..16276
                     /gene="fusA"
                     /locus_tag="PC460_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2601625.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6411 a         2033 c         3339 g         4567 t
ORIGIN      
        1 gataatatag gagcattatt aagaggagta caaagaaacg agatagaaag aggacaagta
       61 ctagcaaaga ctggatcagt aaaggcacac acaaagttta cagcagaagt atatgtactt
      121 aaaaaagaag agggtggaag acatacacca ttctttgatg gatatagacc acaattctat
      181 ttcagaacaa cagacgtaac aggagcttgt aagttaccag aaggaataga gatggtaatg
      241 cctggagata acgtaacaat ggaagtagac ttaataaact caatagttgt agaagaggga
      301 ttaagattct caataagaga aggtggaaga acagtagctt caggagttgt tgctacaata
      361 atagagtaat tctttactaa actgatggat taaaaggaat aacaaaaaga ggggttatgc
      421 tcctcttttt ttaacataga aatacaaaaa cattgacaca gcataggatt ttgtgataac
      481 ttatacaagg taatgattat aggaagttaa taatatcttg aatactgtag atttttaata
      541 aatacatatt ttaaagatat tatattcaca tatattaaca atatatatta ttgtattaca
      601 aagttggagg tgtatcagat gagagttaaa gtaactttag catgtacaga gtgtaagcaa
      661 agaaactaca acactactaa gaataagaaa aataatccag acagaataga attacaaaaa
      721 tattgtagat tctgtaaaaa gcatacaact cataaagaaa caaaatagtt ttttataagg
      781 atgtgagtat ggaatggctg cccaaaaaaa tgacggtgca aaaactaaaa aaaggtttag
      841 tttgtttgga tacttaaaag aaactaaaca agaattaaaa agagttacat ggccgacaaa
      901 aaaggaactg tttaaaaaca caagtatagt tttaactgta gttatttcat gcactatatt
      961 agtatggggt atagacacta tattatctgg tgcactagcg ttattactaa aatagacgaa
     1021 aagaggtaag ttaaatgtca gaattacaag aagcaagttg gtatgtagtt catacctatt
     1081 cgggacatga aaataaagtt aaagctacaa ttgagaaagc agttaaaaca agaggtatgg
     1141 aagactgcat aagacaagta gttgttccaa cagaagaagt agtggaaact actaaaactg
     1201 gaaaagaaaa aactagacaa cgtaaagttt atccaagtta tgttttggtt aaaatgataa
     1261 taactgatga atcttggtat gtagtaagaa atactaaggg agttacaggt ttcgttggac
     1321 ctggttcaaa accagtgcca ttaagtgaag acgaagtcaa agctatgggg atagacacaa
     1381 cagaccctaa agtagtaaat agcgatattg attttgaaat aggagataca gtaaaagtat
     1441 ctcaaggacc atttagtgga caaataggta acatagaaga aattgactta gaaaatagag
     1501 aagtcaaagt atgcataaat gcatttggta aaaggactct atttgtaata gagcttgaag
     1561 gtatagaaaa aatctaatac tatattatag aaaaatctga ggaggtgcgc tcaaaatggc
     1621 taaaaaagtt ataggtcaaa taaaattaca aatacctgca ggaaaggcta ctccagcgcc
     1681 accagttgga ccagcattag gacaacatgg tgttaatata atgggattca caaaagaatt
     1741 taatgctaaa actgcagatc aagctggaat gataatacca gttgttataa ctgtatatca
     1801 agatagatca ttcagtttta taacaaaaac tccaccagct gcagttttaa ttaaaaaagc
     1861 attaaactta aaatcaggtt ctggagaacc aaacaagaaa aaagttgcta aaatgacttc
     1921 agctcaagta agagaaatag ctgaattaaa gatgcctgac ttaaatgctg catcagtaga
     1981 agctgctatg agtatgatag ctggtactgc tagaagcatg ggtgttgtaa tcgaagacta
     2041 gttaaaagat acactttttt ttataaagtg ggaggctaga agccgaaatt aaccacgagg
     2101 aggaaaataa aatggctaaa aaaggtaaaa gatatgcagg tgcattacaa aaagtagata
     2161 gaactaaatt ttatgatgca tcagaagcat taacattagt ttcagatata gctggagcta
     2221 aatttgatga gacagtagaa gcacacatta aattaggtgt tgactcaaga catgctgacc
     2281 aacaagtaag aggtgcagtt gtattacctc atggaactgg taaaacaaaa agagttttag
     2341 tttttgctaa aggtgaaaaa gctaaagaag ctgaacaagc tggtgctgac tttgtaggag
     2401 ctgaagaatt agttcaaaaa atacaaggtg aaaactggtt tgactttgat atagtagttg
     2461 ctactccaga tatgatgggt gtagtaggta gattaggtag agtattagga cctaaaggtt
     2521 taatgccaaa ccctaaatca ggaacagtta catttgatgt agctaaagct atagatgaaa
     2581 taaaagctgg taaagttgaa tacagattag acaaaactaa cataatacac gttccagtag
     2641 gaaaagtatc atttggtgga gaaaaattaa ctgaaaactt tactgcatta atggatgcta
     2701 taataaaagc taaaccagct gctgctaaag gacaatattt aagaagtata acagtagctt
     2761 cttcaatggg acctggagtt aaaataaacc cagcaagaac agctgaataa taaaactgtt
     2821 gacacaggtt gacaaaaatg ttattatatc acttgtcaat gtaaaaaaga atagatttgc
     2881 cgtagatagt gggtgcgaaa gcttaatatc ccgccgaggt tttagataaa ttgagattac
     2941 taaagctttt gatgtctact tttggcaaca aaagcttttt ataatgttgc ggtaaatact
     3001 aagcctattt ggtgaaggag gtgcacatct atatgagaaa agctatagaa attaaatcag
     3061 aagttgtttc tgagatagtt gaaaagttac aaaaatcttc tgctgctgtt gttgtagatt
     3121 acaaaggatt aacagttgaa gaagtaactg agttaagaaa acaaatgaga gaagctggtg
     3181 tagattacaa agtatacaaa aatactttag ttagaagagc agctaaagaa gttggtatag
     3241 agcaatttaa tgatgaatta ttagttggaa ctaatgccat agcatttgga tatgatgatc
     3301 cagttgctcc agctagaata ttaaaaggat tcatggattc tcatccaaaa atgaaattaa
     3361 agatgggtat tgtggaaggt gcattctatg atgaatctaa gattgttgaa atggctaaca
     3421 taccatcaag agaagttctt atcgctaaat tacttggtag cttaaaagct ccagtatcaa
     3481 acttcgcata cttaatagat gcaatagcga aaaaggcaga aggtcaagaa gaagcttaat
     3541 tgcttaacta aaaaattata aatattaatt tgaaataaaa ttcggaggtg ctaaaaatga
     3601 caatagaaca aatattagaa gctatagaaa atatgaaagt tttagaatta aatgaattag
     3661 ttaaagctgc tgaagaaaaa ttcggtgtat cagcttcagc tccagtaatg gtagctggtg
     3721 cagctgctgg tggaccagct gctgaagaaa aaactgagtt tgatgtagta ttaactgacg
     3781 ttggttcttc aaaagttgga gttataaaag cagttagaga aataacagga ttaggattaa
     3841 aagaagctaa agaagtagtt gacaatgctc ctaagacagt taaagaagga gcttctaaag
     3901 aagaagctga tcaaataaaa gagaaattag aagctgctgg agctaaagta gaagttaagt
     3961 agtttcttta aaaagatacc cattgggtat ctttttttat ttataaaaaa ctataaagaa
     4021 agtatttgga attataaaga aagtattttt ataaaataaa aaaaatttat ataatagtac
     4081 taaattaagg gtatcatata tggtgtaatg gtattttaat ttaacattat tatcaaatta
     4141 aaatataaac aattgttgta atgtctatat ataaagtata taattaaact aaaatgtaaa
     4201 attttacatt ttagtttaaa aaaatacacg aatgttttat aaataaaatg taataaataa
     4261 ttaattaaca aatataaatt ttaaaataag ttagatattt agaaaaatcg aaatttgtag
     4321 gatttaaagg acatattcaa ggaggagtta ataatggaaa aattacaagg aaaaattgca
     4381 gtagttactg cagcaacaaa aggtattgga ttagcatcag cagagatatt agcaaaaaat
     4441 ggagcaactg tgtacttagc agctcgttca gaagaattag ctaatgaagt tataaataaa
     4501 ataagtgcag aaggtggttg tgctaagttt gtttacttta atgctcgtga agaagagacc
     4561 tttacctcaa tgatagaaga ggtagttaaa aaagaaggta agatagatat acttgtaaac
     4621 aattttggtt caacaaaccc ttctcttgac aaagaccttg taactgggga tacagataat
     4681 ttctttgaca cagtaaatac taatttaaaa agtgtgtatt taccatgtaa agcagcaatt
     4741 cctcatatga taaagaatgg aaaaggaagt atagtaaata tatcaagtat aggttcagta
     4801 ttacctgatt tatctagaat agcttactgt gtatcaaaag cagcaatcaa ctcattaact
     4861 caaaatatag ctacacaata tgctaaagac aatgttagat gtaatgctgt acttccaggg
     4921 cttattgcaa ctaaagcagc attagacaat atgtctccag agttcataaa agaattttta
     4981 aagcatgttc ctttaaatcg tataggggaa ccagatgata tagcaaaagc tgttctattc
     5041 tatgctagtg atgactcatc atttataaca ggggatttac ttgaagttgc aggaggattt
     5101 ggtttaccta ctccacaatt tgcagataat atattaggat aattaaatta ctaatatgca
     5161 atgaaagctt atgcattagt tatagtagaa atgatatttt tataaaggcc atagatataa
     5221 tttatattgg aaaggtttga ggttcaaaat tccaagttaa atttatttat ggtctttatt
     5281 ttgtatattt taataaattg tatatatttg tataaatgta attatttaca atcattttaa
     5341 tatggaatta taattaaata ggggggatgg tacttgtata aaatttttta tagaaaatta
     5401 gacctataaa tgaaaaatat ggattttata ttggaaaatt gtacataata cataatatat
     5461 gcttaaaata atttacaaaa aatcaaaaat aatgcatagt ttacatataa gataaaaaat
     5521 atattgaaaa atacttgaca ttatgctaaa attattagat gcgttagaat gaactattgt
     5581 tttattttta attattgaga ggtgaaaagt gaatgccaca tcctgtcacg ataggtaaaa
     5641 gaactagaat gagcttttct aaaattaagg aaatagctga tgtgccaaat cttatagaaa
     5701 ttcaagtaga ttcctatgag tggtttttaa aagaaggttt aaaagaagtg tttgatgata
     5761 tttcaccaat agaagattat actggtaatc ttatattgga atttgtcgac tattctctag
     5821 atgataaacc aaagtacgat atagaagagt gtaaagagag agatgctaca tattgtgcac
     5881 ctctaaaggt taaggtaaga cttataaata aagaaacagg tgagataaaa gaacaagaag
     5941 tgtttatggg tgacttccct ttaatgacag aaagaggaac tttcgttata aatggagcag
     6001 aaagagttat agtaagtcaa ttagttagat ctcctggtgt ttactatgcg gaagaaagag
     6061 ataaaacagg taagagattg atttcatcta ctgtaattcc taatagagga gcatggttag
     6121 aatatgaaac agattctaat gatgtaatat ctgtaagagt tgatagaaca agaaaacaac
     6181 cagttacagt tttacttaga gctttaggaa taggaacaga tgcagagata attgaccttt
     6241 taggagaaga tgagagatta tctgctacat tagaaaaaga taatactaag acagtagaag
     6301 aaggtcttgt agagatatac aaaaaattaa gaccaggtga acctcctact gtagaaagtg
     6361 catcatcttt attaaatgct ttattctttg acccaaaaag atatgaccta gcaaaagttg
     6421 gtagatataa attcaataag aaacttgctt tatgttacag aataatgaat aaaatttcag
     6481 cagaagacat aataaatcca gaaactggag aagtatttgt aaaagctgga gaaaaaataa
     6541 gctatgattt ggctaaagcc atacaaaatg caggtataaa tgtagtaaat ctactaatgg
     6601 atgatgataa aaaagttaga gttataggaa ataattttgt ggatattaag tctcacattg
     6661 attttgacat agatgattta aacataaaag aaaaagtaca ttatccaact ttaaaagaaa
     6721 ttttagatgg atatagtgat gaagaagaaa taaaagaggc tataaaatca agaattaaag
     6781 aacttatacc aaaacatata ttattagatg atataatagc ttcaataagt tatgagttca
     6841 atatattcta taatatagga aatattgatg atatagatca cctaggaaac agaagaataa
     6901 gatctgtagg tgaattgtta caaaatcaag ttagaattgg tctttcaaga atggaaagag
     6961 ttataaaaga aagaatgacg gttcaagata tggaagctat aacgcctcaa gcgttagtta
     7021 acataagacc agtttcagct gcaataaaag aattttttgg tagttctcag ttatctcagt
     7081 tcatggatca aacaaatcct ctatctgagt taacgcataa gagaagatta tcagcccttg
     7141 ggcctggagg tctttcaaga gaaagagctg gattcgaagt gcgtgacgtt caccattcac
     7201 actatggtag aatgtgtcca atagagactc cagagggacc aaatatcggt cttataaact
     7261 ctctaggaac ttatgcaaaa ataaatgaat ttggatttat agaatcacca tatagaaaat
     7321 ttgataaaga aacatcaaca gttactgatg aaatacatta tttaactgct gacgaagaag
     7381 atttattcgt aagagctcag gcaaatgagc cattaacaga agatggtaaa ttcgtaaacc
     7441 atagagttgt ttgtagaact gtaaatggtg ctgttgaaat ggttcctgaa agtagagtag
     7501 attacatgga tatatctcct aagcaggttg tatctgttgc cactgctatg atacctttct
     7561 tagaaaatga tgatgccaac cgtgccctta tgggagcgaa catgcagcgt caggcagtgc
     7621 ctctagttag aagagaagca ccaattatag gaactggtat agaatacaga gcagcaaaag
     7681 actctggagc agttgttgtt gctagaaatt ctggtatagc agaaagagta acagcagatg
     7741 aaataattat aaaaagagaa gatggaaaca gagatagata taatctactt aagtttaaac
     7801 gttcaaactc aggtacttgt ataaatcaaa cacctataat aaataagggt gaccagatag
     7861 taaaaggtga tgttatagca gatggtccag caactgattt aggagaagtt gcacttggaa
     7921 gaaactgtct tatagcattc atgacttggg aaggttataa ctacgaggat gctatattaa
     7981 taaatgaaag attagttaaa gaagatagat tatcaacaat tcatatagaa gaatatgaat
     8041 gtgaagctag agatacaaaa ctagggccag aggaaataac tagagatata cctaatgttg
     8101 gagatagtgc aattaagaac ttagatgaca gaggtataat aagaataggt gcagaagtag
     8161 actcaggtga tatattagtt ggtaaagtaa ctccaaaagg agaaactgaa cttactgctg
     8221 aggaaagatt acttcgtgca atatttggag aaaaggctag agaagttaga gatacttcac
     8281 ttaaagtgcc tcatggtgaa tctggtataa tagttgatgt aaaagtattt actagagaaa
     8341 atggagatga tttatctcca ggagtaaatg aattagtaag atgctatata gctaaaaaga
     8401 gaaaaataaa agttggagat aaaatggctg gtcgtcatgg taataaaggg gttatctcaa
     8461 gagtattacc tgaagaagat atgccattca tggaaaatgg aacaccacta gacataatac
     8521 ttaacccaca aggtatacca tcacgtatga acattggtca ggttttagaa gttcatttag
     8581 gacttgctgc taagacatta ggatggtatg tagcaacatc agtattcgat ggtgctaatg
     8641 agtatgacat aatggatgct cttgaagaag ctgggtatcc tagagatggt aaattaactt
     8701 tatatgatgg tagaacaggt gagtcttttg acaacagaat aactgttgga tatatgtact
     8761 acttgaaact acatcactta gtagatgaaa aactacatgc aagaagtact ggaccatact
     8821 cattagttac tcaacagcca ctaggtggta aagcacaatt tggtggacaa agatttggag
     8881 agatggaggt ttgggctctg gaagcatatg gagctgctca catacttcaa gaaatactta
     8941 ctgttaagtc tgatgacgtt gtaggtcgtg ttagaacata tgaagctata gttaaaggtg
     9001 aaaatatacc tgaaccagga atcccagaat catttaaggt tcttataaaa gaacttcaaa
     9061 gtttatgctt agatgtaaaa gtattgacag atgaagacca agaaatagaa gtaagagaat
     9121 ctgtggatga agatgataca ataggtgaat ttgagctaga tgttgtaaat catatgggag
     9181 aagttgaaga aagtaatata atagaagaaa ttgaagatga ttttgcagaa aatgcagaag
     9241 atgaggatat agagaattta gaagaattca ctgaagatga tttatttgaa gaagaaatag
     9301 attttgatag tgatgacttt gatatgtaaa tttaattaat gtaaatctca aatagaaggg
     9361 agagaactcc ttgtttgaat taaacaattt cgagtcgata aaaatagcat tggcttctcc
     9421 agaaaaaata agacaatggt ctaggggaga agttaaaaag ccagaaacta taaattaccg
     9481 tactttaaaa ccagaaaaag atggtctttt ctgtgaaaga atatttggac cacaaaaaga
     9541 ctgggagtgc cactgtggta aatatagaag agttagatat aaaggtgtag tttgtgatag
     9601 atgtggagta gaagtaacta aatcaaaagt aagaagagag agaatgggac atatagagct
     9661 agcggctcct atgtctcaca tctggtactt caaaggtata ccaagtagaa tgggactttt
     9721 acttgatatg tcaccaagat cgttagaaaa aatattatac tttgcctcat atgtggtagt
     9781 tgatccagga gagactggat taaatgaaaa acaattgctt acagaaaaag aatacagaac
     9841 tgctcttgaa aagtatggat atacttttac tgtaggaatg ggtgctgaag ctgtaaagac
     9901 attactacaa aatatagatt tagagcaaca aagtaaagac ctaagagcgg agttaaaaga
     9961 tagtacaggg caaaagaaag ttagaacaat aagaagatta gaagttgtag aggcatttaa
    10021 aaagtctgga aataaaccag aatggatgat tttagatgca ataccagtaa taccaccaga
    10081 tttaagacca atggtacaac ttgatggtgg aagatttgcg acttcagacc taaatgattt
    10141 atatagaaga gttataaata gaaacaatag acttaaaaga ttattagagc ttggagctcc
    10201 agatataatt gtaagaaatg aaaaaagaat gcttcaagaa gctgttgatg cattgataga
    10261 taatggtaga agaggtagac ctgtaacagg acctggaaat agaccactta aatctttatc
    10321 agatatgtta aaaggtaagc aaggtcgttt ccgtcaaaac ttacttggta agcgtgttga
    10381 ctactcagga cgttctgtta tagttgttgg accagaactt aaattttatc aatgtggact
    10441 tccaaagaag atggcattag aattattcaa gccatttgtt atggataaat tagttaaaga
    10501 aggttatgca cataatataa aaagtgcgaa atctatagta gaaaaagtta agccagaagt
    10561 ttgggatgtt ttagaagatg ttataaaaag tcatccagtt cttcttaacc gtgcaccgac
    10621 tctacataga ttaggtatac aagcatttga accaatctta gttgaaggta aggctataaa
    10681 actacatcct cttgtatgta cagcttataa tgcagacttt gatggtgacc aaatggcagt
    10741 tcatgtacct ttatcagtag aagcacaagc agaagcaaga ttcttaatgc tttctgtaaa
    10801 taatatactt gctcctaaag atggttcacc tataactact ccatctcagg atatggtttt
    10861 aggttgctat tatctaacaa tagaagccca agatggagct aaaggaacag gtatggtatt
    10921 taaagacttt aatgaactat tacttgctta ttacaataaa tcagttcatc tacatgcatt
    10981 agtgaaactt aaggtaacac ttgaagatgg aagaagttca ttggttgaaa gtactgttgg
    11041 tagatttata tttaatgaaa atatacctca agacttaggt tttgtagata gaaaagaaaa
    11101 tccatttgca cttgaagttg atttcttagc agataaaaaa tctcttggta aaataataga
    11161 taaatgcttt agaaaacacg gaaatacaga aacagctgaa ttactagatt atataaaagc
    11221 tctaggattt aaatattcta cattaggtgg cataacagtt gctgttgatg atatgagtgt
    11281 cccagaagaa aagaaagtat ttatagctga ggcagaagct aaagttgata agtatgaaaa
    11341 agcatacaga agaggtctaa tctctgatga agaaagatat gaaaaagtta tagagacatg
    11401 gacagaaaca actgataaag ttactgatgc tcttatgggt ggactagata gattaaataa
    11461 tatatatata atggcacatt caggagccag aggttctaaa aaccaaatta gacagctggc
    11521 aggtatgcgt ggtcttatgg caaatgcatc tggtaaaaca gttgagatac cagttaaatc
    11581 taatttccgt gaaggtttat cagtactaga atactttaca tcttcacatg gtgccagaaa
    11641 aggtcttgcc gatacagcta tacgtacagc tgaatctgga tatttaacaa gaagacttgt
    11701 tgatgtaagt caagatgtta ttgtaaggga aatagattgt ggaacagaag atactacaga
    11761 gatttatgct ataaaagaag gaaatgaagt tatagaagag atatatgata gaattgtagg
    11821 aagatatact atagacccta tattaaatcc tgaaactgga gaagttatag ttgaggctga
    11881 ttcaatgata caagaagatg aagcagaaac tatagtagct ttaggaattg aaaaaattag
    11941 gataagaaca gttcttaact gtaaaactaa tcatggagtt tgttctaagt gttatggtag
    12001 aaacttggca acaggaaaag aagttaatat aggtgaagca gttggtataa tagcagctca
    12061 atctatcggt gagccgggta ctcagcttac aatgcgtaca ttccatactg gaggagttgc
    12121 aggagctgat ataactcaag gtctaccaag ggttgaagaa ttatttgaag ctagaaaacc
    12181 aaaaggatta gctgtaataa ctgaagtatc tggtagagtt gaaattgatg aaacaggaaa
    12241 gagaaaagaa gtaaatgtaa taccagaaga aggcgagact caaacatatg taattccata
    12301 tggttctaga ttaaaagtta agcaaggtca aatgctagag gctggagacc ctctaacaca
    12361 aggatttata aatcctcatg atatagtaag agtaaatggt gttaagggag ttcaagaata
    12421 tatagttaaa gaagttcaaa gagtgtatag acttcaaggg gttgatgtta atgataagca
    12481 tatagaagtt atagtaagac aaatgctatc taaagtaaaa gttgaagacc caggagatac
    12541 agatttattg ccaggtggat atgaagatgt attaacattc aatgaatgta ataaagatgc
    12601 tatagataaa ggtctaagac cagcagttgc taaaagagtt ttacttggta taactaaagc
    12661 atctcttgca actgattcat tcttatcagc agcttctttc caagaaacaa caagagtatt
    12721 aacagaggca gcaataaaag gtaaagaaga ccacttaata ggacttaaag aaaatgttat
    12781 attaggtaag ttaataccag caggaacagg aatgaagaaa tatagaaata tagctgttga
    12841 aaaaattgaa gattaatagc atatagagta aagaaatctt gacaaccatt atttgtggtg
    12901 ttaaaatagt aaagtgtgtt gtttaaaagt acactaaggg cttgaacaag ttcttagtga
    12961 cttttagatg gcaataaatg agaactaaaa agcatcaagc tagaaaagtg ctaatatcac
    13021 atatttatgt gttatagaca ttaaattgta agtaacgaat gttacaatca gtcagtatga
    13081 atattcatat tgatgtagtt ggcggtcact gataagatcg aaaatttgga aggaggtgca
    13141 gatgatgcca acaattaacc aattagttcg taaaagtaga aaagcgctag agaagaaatc
    13201 tactgctcca gcattacaaa aaggttacaa ctctttaaat aagaaagtaa ctgacgctag
    13261 tgccccacaa aaaagagggg tttgtacttc agtaaaaaca gttactccta agaagcctaa
    13321 ctcagcttta agaaaagttg ccagagttag attaactaat ggtatagaag tttctgctta
    13381 tataccagga gaaggacaca acttacaaga gcatagtgtt gttcttataa gaggaggaag
    13441 agtaaaagac cttccagggg ttagatacca tatactaaga ggtactctag atacagcagg
    13501 tgttgataag agaagacaat caagatctaa atacggtgcg aagagaccta aagaagctaa
    13561 aaagtaaaaa caattaatga ttgattatag ttcggtgctt taaatatata tatatttacg
    13621 gcatcacggc attaaatgat ttatgtcgag tacctatgat ttaagttaaa taattaagga
    13681 gggaagcgaa atgccaagaa aaggtaatat tccaaaaaga gaagttttac cagatcctat
    13741 gtatgggagt aaggtagtta ctaaattaat aaataatcta atggtagacg gaaaaaaagg
    13801 aaagtctcaa agaatagttt atgatgcttt cgcaattgtt gctgaaaaga caggagaaga
    13861 agctttagaa gtatttaata aagcaatgga taatataatg cctgttttag aagtaaaagc
    13921 aagaagagtt ggtggggcaa actaccaagt gccaatcgaa gttagacctg aaagaagaca
    13981 aacattaggt ttaagatggc tagtaaaata tactagagct cgtggagaaa aaggaatggt
    14041 tgaaaagcta gctaaagaga taatggacgc agctaacaac actggagctt cagttaagaa
    14101 aaaagaagat actcataaaa tggcagaagc taataaagca tttgctcatt acagatggta
    14161 ggatatatag atatcttata aaattgagga tatcataagg aggataacca tggctagaaa
    14221 gtttcctttg gaaagaacta ggaatatagg aatcatggct catatagatg caggaaaaac
    14281 tactactaca gaaagaatcc tgttttatac tgggcaaact cataaaatag gagaaactca
    14341 tgaaggagct tctcaaatgg actggatgga gcaagaaaag gaaagaggta taacaataac
    14401 ttctgccgct actactgctt catggaaaga ccatagaata aacataatag atactcctgg
    14461 acacgtggac ttcacagttg aggttgaaag atctctaaga gttcttgacg gttcagttgc
    14521 tgtattctgt gcaaaaggtg gggttgagcc tcaatcagaa aatgtatgga gacaagctga
    14581 aacttatgga gtacctagaa tagcttttgt aaataaaatg gatatattag gtgcagattt
    14641 ctacaatgtt gtaagcatga tgaaatctag attaaattct aatgcagttc ctatgcaatt
    14701 accaataggt aaagaagatt catttatagg tataatagac ttattaaaaa tggatgctgt
    14761 tatatacaaa gacgacctag gggttgaaat ggaagaaaca gatataccag aagatatgaa
    14821 agaattagct gctgagtgga gagaaaaatt agttgaatca gtagctgaaa ctgatgaaga
    14881 attaatgatg aaataccttg aaggtgaaga acttactata gatgaattaa aagttgctat
    14941 aagaaaagct actatagctt gtgaaatgaa cccagttttc tgtggtacag cttatagaaa
    15001 caaaggtgtt cagttagtaa tagatgcagt tttagattac ttaccagcgc caactgatat
    15061 accagctata aaaggtatat tagcagatgg agaagaagct gaaagacatt cttctgatga
    15121 agagccattc tctgcattag cattcaaaat aatgactgac ccattcgttg gaaaactagc
    15181 gttcttcaga gtttattctg gaacattaga atcaggttct tatgttttaa atgcaactaa
    15241 gaataagaga gaaagaattg gtcgtatatt acaaatgcat gctaatacaa gagaagaaat
    15301 aactaaagtg tatgctggag atatagcagc agcagtagga ttaaaagata ctactactgg
    15361 agacacatta tgtgaccctg ctaatccaat aatacttgaa tctatggaat tccctgagcc
    15421 agttatatct gttgctatag aaccaagttc taaagcagct caagaaaaaa tgggtatagc
    15481 tcttcaaaaa ttagctgaag aagacccaac tttcacagtt aagactgacc aagaaacagg
    15541 acaaactata atatctggta tgggtgaatt acacttagag ataatagtag atagattatt
    15601 aagagaattt aaagtagaag ctaaagttgg tgcaccacaa gttgcatata gagagactat
    15661 aactcaacca gttgatgttg aatacaagta ctctaagcaa tcaggtggta gaggacaata
    15721 cggtcacgtt aagattagag ttgctcctca agagccaggt gaaggataca aattcacaaa
    15781 caaaactgtt ggtggatcag taccaaaaga atatgttggt ccagttgaca tgggtataca
    15841 aggagctatg caatctggta tagttgctgg atatccagtt gtagacgttg ctgttgagtt
    15901 atatgatggt tcttaccatg aggttgactc ttctgagatg gcgtttaaga tggctggttc
    15961 aatggcattc aaagatgcta tgaagaaagg taatgcagtt cttttagagc cttacttcaa
    16021 agtagaagtt gttactccag aagattacat gggagatgtt atgggtgact taaactcaag
    16081 aagaggatta atccaaggta tggaagctag aagtggtgca caagttataa atgcattcgt
    16141 tccactttca gaaatgtttg gatactcaac agacctaaga tcttcaactc aaggtcgtgc
    16201 aacttacaca atgatattcg atcactatga gcaagttcca gcttcagttg ctaagaaaat
    16261 agctgaagga aaataattta aaaataatta ataatataaa tataaaaaat aatcagctag
    16321 atgagagata tctctcatct agcacaagat
//
LOCUS       DAMJWU010000082        15947 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMJWU010000082 DAMJWU010000000
VERSION     DAMJWU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.085"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            71..667
                     /locus_tag="PC460_002749"
     CDS             71..667
                     /locus_tag="PC460_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF2601626.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(765..1724)
                     /locus_tag="PC460_002750"
     CDS             complement(765..1724)
                     /locus_tag="PC460_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2601627.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1758..2489)
                     /locus_tag="PC460_002751"
     CDS             complement(1758..2489)
                     /locus_tag="PC460_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601628.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2482..3252)
                     /locus_tag="PC460_002752"
     CDS             complement(2482..3252)
                     /locus_tag="PC460_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601629.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3337..4047)
                     /locus_tag="PC460_002753"
     CDS             complement(3337..4047)
                     /locus_tag="PC460_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2601630.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(4227..4379)
                     /locus_tag="PC460_002754"
     CDS             complement(4227..4379)
                     /locus_tag="PC460_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF2601631.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(4405..6561)
                     /gene="feoB"
                     /locus_tag="PC460_002755"
     CDS             complement(4405..6561)
                     /gene="feoB"
                     /locus_tag="PC460_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2601632.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6661..6882)
                     /locus_tag="PC460_002756"
     CDS             complement(6661..6882)
                     /locus_tag="PC460_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2601633.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6895..7107)
                     /locus_tag="PC460_002757"
     CDS             complement(6895..7107)
                     /locus_tag="PC460_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2601634.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7428..10325)
                     /locus_tag="PC460_002758"
     CDS             complement(7428..10325)
                     /locus_tag="PC460_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2601635.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLNSGYEYSVLYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKYKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSNLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKANMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLKKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(10652..11260)
                     /locus_tag="PC460_002759"
     CDS             complement(10652..11260)
                     /locus_tag="PC460_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF2601636.1"
                     /translation="MVDNLIKYIHKSYVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(11520..12065)
                     /locus_tag="PC460_002760"
     CDS             complement(11520..12065)
                     /locus_tag="PC460_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2601637.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(12183..14006)
                     /locus_tag="PC460_002761"
     CDS             complement(12183..14006)
                     /locus_tag="PC460_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601638.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(14019..15794)
                     /locus_tag="PC460_002762"
     CDS             complement(14019..15794)
                     /locus_tag="PC460_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601639.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         5297 a         2832 c         1564 g         6254 t
ORIGIN      
        1 atattgatta tcattcatag tataatctaa ttacttgata ttaattttca tttgaaggag
       61 gttatctttt atgtcttgtt gtaattcaca attttgttgt aaaaataaaa ttgaatcatc
      121 atatataaaa tggcttttag aagttttagg tccagtcttt ttaggctcta aacctgctga
      181 aataataaat atttcgttta ctgatgtaaa tagagaggaa aaaattaatg atatttataa
      241 atatctatat aaatgtaaga aaatagattt tatagttatt gataaagaaa aaaagggatt
      301 aaaaatctta tttgtaaata aaaaagcatt atcagaaaaa ttaaaatgta aaaaaacagt
      361 aaacttttta aagtttttag gatacaaaca aaatacgaat gttaatgctt atttagagca
      421 tttagtagat aaacttaaga gtgatgtttt tcctgatgaa ataggtattt ttttaggata
      481 tcctcttaaa gatgtgattg gatttatggg atatagtaat tatgaagttt ctatgattaa
      541 atattggaaa gtttatggtg acacaaaaca atccgaggac acatattcga agtttttact
      601 tcaccgtaag aaaatgagaa aattactaga ctacataagt gtagataaaa tagtaagctg
      661 tttttaattg tatttttgta taataataaa aatacaccct cttttaccat atagtttcca
      721 taataaataa gggtgtattt tccactttaa tattttaaag tattctaatt ttgattgtac
      781 aacatatctt taatatttat tttcttttgt tgcattccat tagacattaa aaattcctgt
      841 gtatcttcta attcttttat atccttgtcc gttattttag aactaaagtc ataccaagaa
      901 tacatttctt ttgtttcatc taaagataaa ccaacttctt tagatacaac atccatcact
      961 tcatccttgt tttcattcat atattttaaa gtttcttcat gaaccttcat aaatcttttt
     1021 actaattcag gatatttttc agcaaagtca tcacttacag cagtaactat tattccactt
     1081 actaatcctt ctccatttac aatcattttg gcacctgaat ttatcgtttt caatgccact
     1141 ggacctgcaa gcattgctac atcagcactt ttactttcta atgcagaagc agcactagga
     1201 atatccatat ttacatattc aacatcatct atagtatagc cttctttagc aagtgcagca
     1261 atcaacagtt gatgaagtat agtccctttt ggacctacaa tttttttacc ttttaagtct
     1321 tttatagatt taatattatc attattagtt ataagcatat atccttttgg tgaacggcta
     1381 taaatattcg taattttaag accaacacca tttgatgcag ctattattgc agacgttcct
     1441 cctaatgcat gtaaaaagtc tagctcacct gctgctaatg cttgagtttg ctctggtcct
     1501 gttgtcaatt catggaaatt gacttttata ttgtcctttt taaattcctt tccaaataaa
     1561 tcatcctttt tttctaatat actaggaaca ttaagtggtg attttacata agttaaattt
     1621 atttccttag gtaaactagc ctcttttttt gcaggttcat ctttcttatc atttgttgtt
     1681 gaacatccta cagtcccaac caacaccatt attaaagatg tcattactgc taaaatcttt
     1741 ttgtttcttt taatcaatta aaactccccc aattccctta atatttcttt tttcaagttt
     1801 atataatagt ttttagttaa atctctatta tattcatctt cgacactaaa ttgtttaatc
     1861 cttttatttt tactaaaaac aattattttc tttgctattt ccatagcttc ttcaatattg
     1921 tgagttacaa atactacacc ttttttagta tttttatgga tatttacaac ttctttttgc
     1981 atttttcttc ttgtgaagta atctagtgct gcaaatggtt cgtccatcag caaaatatcg
     2041 ggattgaagg aaagtgctct tgctatactt actctgtgag ccattcctcc tgataattca
     2101 tttggatatg agtttttaaa cttttcaagc ttcatcattt ttaaatactt gtctaaatca
     2161 actttatttc tattatcttt ttcagtatgt aaaagtatat tttcacttac attcagccat
     2221 ggcataagtc tactttcttg aaataccatt cctacttttg gagtacactt tttatcatta
     2281 ttaaaaaaat atatgtttcc actagtagca ttttcaagcc ctgctaaaat tctaagtagc
     2341 gttgttttcc cacatccact ttcacctaat ataacagtta tctcttcact agatatattt
     2401 aaagatatat tatcaagaac taaatgctct ttattatcaa ctaaatattt cttatatata
     2461 ttctctagca aaaatccact tctaaccatt taaatcaacc tgcttccctt tgctaaattt
     2521 tgttattatc tttgaaaata gataatctga aagtatacct aaaaatccaa taacaaaaat
     2581 tcctaccaat acaacatcag ttctagacat atcttttcca tcagaaatca aatatcctag
     2641 tcctgaagaa gctgcaataa gctctgctcc aattatagcc ctaaaactat acccaagccc
     2701 taaactaagt ccaacaacaa tatccagcat agaattaggg attattattt tataaaaaat
     2761 tctcttttta gataaattaa aagatttacc tacttctatt aatttattgt cacaattttt
     2821 tatacctttc aatgtattta aaaatattgg aaaaaaggat gccaaaataa taattataat
     2881 tttagattct tcacctatac caaaccataa aataagcatt ggcaccaaag ctagtggagg
     2941 tgtacttcgc ataaattcaa atagtggttt gaaatattca tacatttttt tatttactcc
     3001 aaataaaact ccaagtggta tagccatcaa tgaacttatt aaaaaaccta ttaatatcct
     3061 tcttacactg gcataaacat ttaagaaaat agaaccgtca tatatcattt ttataaaagt
     3121 atctaagact atttcaggag gaggcagaat atagctactc catataccca aaacacatac
     3181 tatcttccat aaaattaaaa ttattaaaaa aatataatat cctttaagcc ttttatgaat
     3241 tttctcaaac atataaaact ccttttaaca tatctatccc tgctaccctt tttatttttt
     3301 aatatcttaa ataataatta agctatatca taagatttaa tctatgccaa gtcttaatcc
     3361 acctgaactc attctatcag aaggtgtaga atctgttttt aagccagaca atctttcaga
     3421 tggttgtata atgactattt gtccaggttc aacaaattcc atatgataag attctcctga
     3481 agtctgacca ataaaagtct tccatgatag ttttgatact ttaggatatg gttctcttcc
     3541 agtccaagct actaatgcat ctgggtcaac acaacaaggt ccttcaagaa ttagaggatt
     3601 accatctgtt attatagcaa catcttgatt ttgacctgtt ttatttacta aatgtgtagt
     3661 aaaaagacct ttttgagata ttacccctga accaatcaat tttacactgt aatttaattg
     3721 ttcagaaaat gccaataagt tttcactctc aacataaatg gaatcacctg ggtcaattga
     3781 tattacatct acatggtaag cattttgagc taggtaaatt tcaccttcac cttcaaccac
     3841 cattattggc atttgttctc ctgtcaatga cctctgaaca tgacctaata atgctcttcc
     3901 caatccaccg ccattacttg gacctaacag caatttttca aaattaaaat ttcctttaaa
     3961 ggctaccata gcaccttttt ttgcaaaaaa tttaccactt ccccttgcta tacacataag
     4021 ctcgtttgta acatcaaatt taaacataat attcacttat attgcttaat ttagcaataa
     4081 tccctcctaa tcatatattt atatataatt atacagtaaa attacagcat agtctaacct
     4141 acttcaaata tgaaatttgt tatttataca aaaaataaga acctgataat aaagcaattt
     4201 aactttttat caggctctta tcctatttat ctcttagcgc tatgacaact cttactactt
     4261 gagcatccag aacaaccaca tccacaactg ctaccattct tttttgaatt tttaaccata
     4321 tacattacag ctagtgccat taatactact acaatagctg ctataataaa tgtagccata
     4381 ttcatcactc cttatttata tgaattaatt tttcatataa cttaatttac ccattttttc
     4441 ttttttcata tttttatttg aataagttaa aactacaaag actactgcta ctgctataaa
     4501 tatagatatt attgccccag caatacttcc tccagtacct aaaactaaac ttccaacttg
     4561 atttataaga agagcaatta tatacgctgt taaagtttgg aatcctaatg taatccatgt
     4621 ccatttccat gaacccattt ctctttttat ggctccaata gctgcaaaac aaggtgcaca
     4681 aagcatatta aatgccataa atgcaaacgc actagctact gtaaacatgc ttgcaactga
     4741 agcaagaagt gttgggtctt gctctgttgc atctgatata ccaaataaaa ctccaaatgt
     4801 tccaactaca ttttccttag ctactagacc tgtaactgtt gctactgatg attgccaatt
     4861 tccaaatcca agaggagcaa atattggtgc aactatattt cctaagctag ctaatatact
     4921 atctccagca tctaccattt gtaatgacca attaaatgat tgtaagaacc agattactcc
     4981 acaagcaaca aatataattg ttcctgcttt gattataaat gcttttcctc tatcccacat
     5041 atgtattaaa acaccttttg cacttggaat atgatattga ggtaattcca taacaaatgg
     5101 agatggttct cctgcaaata aactagtttt ctttaatata attccacaaa tgattatcat
     5161 tgcaatacct aagaaataca ttgatggagc tacccatgaa gcacctccaa ataatgcccc
     5221 tgcaaataat gctattattg gaatcttagc tccacatgga atgaatgtag ttaacataat
     5281 agtcattttt ctatctctat cattctctat agtacgagtt gacataactc ctggaactcc
     5341 acaacctgaa cttataagca ttggtataaa tgattttcct gaaagaccaa acttacggaa
     5401 aattctatcc attataaaag ctacacgtga catgtaccca caatcttcta atattgataa
     5461 taataagaat aaaagcatga tttgtggtac aaatcctagt actgctccaa ctccaccaat
     5521 tagaccatca actacaagtc cttgtaacca ctctgctaca tttaaagaag ctagaaaatt
     5581 acttacattg ccttgaataa tttctccaaa cagtacatca tttgtccaat cagttgctat
     5641 agtacccaat gaacttactg ctatatagta aactccccac attataagtg caaaaatagg
     5701 taatgctagt atacgatttg ttacaatctt atctatctta tctgatacag tttccttacc
     5761 ttttcgattc ttctttatta tacttgaaat tattgaacta ataaattcat aacgattggc
     5821 tgttattata ctttcactat catcatccaa ttcatcttca caatttctag ttatttcttc
     5881 aatactattc aatattgttt tagaaatatc aagtttttgt ataacatttt catctctctc
     5941 aaatagcttt atagctaacc agcgtgtttc tacatctata tatggagtct tttcttctat
     6001 tattttttca attttttcta ttgcatcctt actttcatca acaaagggta atttgaaatt
     6061 taatttattg ttactacttg caagttcaac tgccttttca gcagcctcca taattccttg
     6121 acctttaact gctgttactt ctaccactgg gcaacctata acttcagata gttctttaat
     6181 atttatctta tcaccattct tatttacaat atccatcata ttaagagcta taacggtcgg
     6241 tatacctagc tctaaaactt gagttgtaag atataaattt ctctctatat ttgatgcatc
     6301 tacaatattt attactgcat ccggcttatc atctagcata aaattacgtg taactacttc
     6361 ttctaatgta tatggagata aagaataaat acctggtaaa tctactattt ccacatcttt
     6421 atttcctttt aattttccac cttttttttc aactgttact cctggccagt taccaacata
     6481 ttgtgatgag cctgtaagcc cattgaacat tgttgtctta ccacagttag gatttcctat
     6541 gagacctatt tttattgaca tttccattcc ctccattatt atttaaatac atcactattc
     6601 atgatataga aaatcattct cattatataa ttacattaac tctactctaa ttaatcttta
     6661 ttattccaca ataatttttt ctgcatctga cttacgaatt gatagttcat aatctctgac
     6721 tgttacctca attgggtcac caagaggagc tacttttctt ataaatatat taacgcctct
     6781 tgtaatcccc atatccatta tacgtcttcg tgtagctcct tctccttcta actttttgac
     6841 ttttacagtc tctccacact taatatcctt cagtctgttc atgtcattac ctccctatat
     6901 tattattcta cttgccattt ttttatctaa tgcaactcta gtatctttta cgtctattat
     6961 taagttacca tctatcttag aaactatttt aacatttgta cctactacaa aacctatact
     7021 atttaaaaaa gtcctcgtct tatcacttcc tgaaattttc tttataacta cttcttcacc
     7081 tgtatttgat agtatcaatg gcatcatata atatccctcc tcttaaacta atttaaatct
     7141 acaagcaatc gttatcttat atataaatta caatacctta aatcttaatc tattaaaata
     7201 atatcctatt ttttccttct ttttacaatt cctaattttg atttaagtct atcacaatag
     7261 tatatttttt gcaagtgatt attgtattct tttgattttt tttctcattt gatttatttt
     7321 attaataaaa tcgtaaaaat taatacaaaa ataaagatgc tctaaaatag tatactcaac
     7381 taatattaaa gcacctttat tatatatatc attacttaca cattgcttta ttctttttta
     7441 aaagcaattt tttcaaactc ttttacaggc attggcttag aaaacaaata tccttgtacc
     7501 atgtcacacc caatttgctt tagaaattct acttgcgaaa tagtctctac accttctgat
     7561 attactttca ttttcaattc ctttgccatt ttaatgacat tagatataac aattttttca
     7621 ttattactct catctttttc tgtaaaaaat gctctatcta gttttaatat gtcaacaggc
     7681 aattctttta aaagattaag agaggaatat cccgtaccaa aatcatccat agagattaca
     7741 aatccctttt ctttcaaatt gttcattgta tcaagtaacc cttctatatt atctaatact
     7801 gctgtttctg ttagctctaa ttctataagt cttgtcggaa ttttgtattt atctacaatt
     7861 aatttaaact tctctataaa attactattt acaaaatgca ttcgagacaa gtttacagat
     7921 ataggtacta ctttttgtcc ttcatccatc cattctctaa tctttttaca cacttcctca
     7981 aatacataga aatcaatatt tacaataaaa ccattcctct caaatacagg aataaattct
     8041 attggtggta ttagaccttt ttgtggatta tcccatcgaa caagagcctc tgcccctata
     8101 atttgataat ctgaaagact atatttaggt tgaaaataaa caataaattc tccattatta
     8161 agagcatcta ccattgaatt ttcaatttct ttctctttta atattttttt atgcatttcc
     8221 ttatcgtaaa aagcaaatga atttttatga ccacctttta tagtttttct tgcagtatta
     8281 gctctatcca taatagtatt tatgtcatta tcacttttac ttattttata tataccacaa
     8341 tctaaaatca atttataata gtttatttta ggattactaa gtatttgaac cttattgtaa
     8401 atattgttta accttgtttt aatatcatca tctgtcttat acttaataag aagaacaaaa
     8461 tggtcagcag aaactcttgc aaaaatttca tcttccttta attcacttgc aatagtattt
     8521 gatatatgta tcaaagtatc atttcccata tcatatccaa acatatcatt tatatattta
     8581 aatttgtcaa catctatata aaacaatgca tattcttctg gattattttt agcaaacagt
     8641 ttattcgaat ttattttaaa tttatctata ctatctgctc ctgtcaatgg gtctgtatat
     8701 gctatcttct ttagataatc ctctttcttc tttttattat aaattatata tttaatagca
     8761 attaagaata aaatacatac aaaagaaata atttgtattg aatattcttt aaaaatcaca
     8821 ccaaatggta ctttataagg tttgtcaata gtattagaat atatacaatc acttaattct
     8881 tcttcagtaa ttttgtctat tgatttattt aaaatagata ctaatatagg gtcagtttta
     8941 tttgataccc ctatacgcat cccatagctt gtatctaaaa cagatataac agataaattc
     9001 tcactctttc cttctcttaa aagttcatca aactgataag aactaagtat cattaagttt
     9061 gctttacctt catttatagc gtttagacaa ctttctatac tcttataaga tgttgtctta
     9121 gcattttcaa aacttttttc tatataagaa tcgatagact tataactact cgttaaagca
     9181 atatttaaat cattattgag attggtatcc agttttccta ccataatcat tggaatattt
     9241 atgtatggat ttgtcaattt catattaaat cgttttgcct tatcattttc attgattgag
     9301 ccacatagca tatttgcctt accacttttt atgtaatcaa gactctcatt ataatttttt
     9361 gttttaatat attcaaattt tataccacat ttttttgata taattgacat taaattggaa
     9421 cttatacctt taaacgaatt gctttcctta tcataatact ctacaggaga ccactctgaa
     9481 tcatatacca tagtaagttt tgggtttgct ttaataaaat tcatttcttg ccgagtaaat
     9541 gctatggaat tattgacctc tcttttgaaa tattttttat ataattccaa ctcataatac
     9601 atatcattta tttttaattc ttttaaagca taatctaatt cttttaccaa attaggttta
     9661 tattttgcag tagcaaaata aaaaggctct actgaaaatt tgctaactat tttcattcct
     9721 ttagcattta taatacttcc acagacaaat gcatctgcct ttttttcatt gacagactca
     9781 attaattcat caatagaatt acagtagata tattcaactg aaaaattatt ttcttctcta
     9841 tacttctcaa atgcaatatt atgaaagttt cctctaagta cagctactct cattccctta
     9901 aaggaactta tatcttcata aaacatatta ttgttactat cttttgtata tagtacacta
     9961 tattcatatc ctgaatttag tttaggaaaa ttaaatagtt tatttctttc atcattcttc
    10021 tgtaaaggtc caagtaagtc tatttcacct ttttctagtc tgtctaaaca ttcttcccaa
    10081 gaccctttta caaattcata ttcccatctt gtatattttg agatttctct tagataatca
    10141 taaccatatc ctgtataaga atgctctttt gatgaggata ttattccgta gtcatcacaa
    10201 tatccaactc ttattttttt tgaattttca gactcatcag caaagcatag tatattgtta
    10261 aaacatgata taataagtaa aaatatcaaa gaaagtatca agaagtgctt tttaaatgct
    10321 ttcatattta aaaacctcca atttgccaac ttactttaac ttatttggtt ttctagttgt
    10381 aactactttt attgtaatgg taaatatatc aaattgcaat attctcatat aatattctta
    10441 ttcaactata atgaatttat tattatctat ttaaatacac tatctaatat tagtttctta
    10501 taagtcataa aagcacaact aacactataa ttctagtgat taattttatt tcaaaaaata
    10561 taataacact actaatattt taaatatcaa tagtgtcata aaaacttttc agattaatta
    10621 aatctttcta tatattttat aataatctaa atcatgctaa atatttagac agaaaacttt
    10681 tttctttaat agtaaatact tttgtttgaa tatcagaata attatttgaa tatctatcta
    10741 taaaaccatg atttgcatct aaaatctcaa tttggatatt ttgtttccta gaaagttggt
    10801 aagaaataga atcaacatca aatgaatcgt gctttgcaaa tactaataag gtaggacatt
    10861 ttggaattac atccatgtaa tctcgaatcc gagagccata acaacaaatt atgccatcac
    10921 aaaaagaatc ttcactgcat ctccatgcaa tagttgctcc cacactatac ccagcaacaa
    10981 atacatattt atactctgat tttaaatcat ttataagctt ttcaactttt ttataaatat
    11041 caaaaccaac attatttata aaaaaatcat atgcctcttg gacttttgaa tatggaaaaa
    11101 ctcttttttc tcccagaagt tcaggacaaa atacatcata tccatattta taatactttt
    11161 gacaaacatc ttctataaag ctattaatcc catagatttc atgtaaaaca acaatagcta
    11221 cataactttt atgtatatac tttatcagat tatccaccac ctttacaaaa cagcaaattt
    11281 tttatactta gtttaatata ttcttcttta catgtcaatt tacttttcta aaatcaaatt
    11341 tatttttgga ttaatcattt atttttaaaa gttgtgcatt attactatta aattattctc
    11401 tattattaaa aatataatcg ttagaaataa aaaataaagt acaaataatt aaaaaatatc
    11461 tcactcataa ctttaaaaat tgtaagtgag atactcctaa aatctacttt atattaaatt
    11521 tattttccaa aatatctatt taataatcct aagaatgctt taccatgtct agcttcatct
    11581 ttacacattt catgtactgt atcatgtata gcatctaatc ctaattcttt tgctttctta
    11641 gctaatttta attttccatc agttgcacca tactcagcat caactctaac tcttaagttt
    11701 tcttttgtat ctgcaactac aacttctcca agaagttctg caaatttagc agcatgttca
    11761 gcttcttcaa gagctattct cttataagct tctcctactt ctggataacc ttctctatca
    11821 gcttgtctac tcattgctaa atacattcct acttctgtac actcaccaac aaagttagct
    11881 cttaatcctt cgattatttc ttcatctaca ccttgagcta ctcctattct gtgctcatca
    11941 gcccaatttt tttcgccttt catttcttca aatttatcag ctccaacttt acatactgga
    12001 cattgtgctg gtgcagcatc tccttcatgt atatatccac atactgtaca aacaaatttc
    12061 ttcataatta ctttcctcct aaattttata tatcaaatta aattttttat ttattaagtt
    12121 agtctatata tacccgcacc atataccttt aaacaatttt tttaaaagtt ttttctaaat
    12181 acttattttc cctgtgtcaa taatatgttt ctataataat gtccctttaa ttttatcaat
    12241 tctttatgat ttcctgattc cacaattcta ccattttcta aaactattat attatcacaa
    12301 tttgatatgg tacttaatct atgagctata attatacttg tctttccata agttaaatta
    12361 tttattgctt cttgaatttt agcttctgtt gctatatcta tgttacttgt agcctcatcc
    12421 aaaataagta ttggagcatt ttttaatact gctctagcaa tacttattaa ttgtttttga
    12481 ccttcactta aatcttcacc cctatccgat aatacagtac tatatccttc acgcatttta
    12541 actataaatt tatgtgcatt tgctatttta ctagcatata taacttcttc atcagtagcc
    12601 tccagatttc cataccttat attttccata actgtatcat tgaatatata accttcttgt
    12661 ggaactactc caaaacaagt tctcaaatag tttctgttaa tagagtatat atccttacca
    12721 tctaaaaata tacttccttc atctatatga taaaaatttg taattagatt tataatagtt
    12781 gtttttccac ttcccgtctc tcctatgatg gcatttgatg tgccagattt gatttttagg
    12841 tttatattat ctaaaatttt cttttttgaa tcataagaaa aacttacatc tttaagctca
    12901 atttctccct ttacaaaatg agagtttaaa ttaccatctt tattatcgtt aatctcactt
    12961 ttaaacatgt tatgcacttc ttgctcttct tgttcatcta taatttcaaa aactctttca
    13021 catccagcta tggctgaaag taatgtatta aatagatttg ctaattcatt taaaggtctt
    13081 gtgaattgtc ttacataact taaaaaacta gctataaccc ctatggttat aatatcttta
    13141 gatgcaagaa tgcctcctat aatacatata gttgcaaatc ctatattact aatcacattc
    13201 ataataggca ttaaaagaga agtgtaaatt tgcgatttaa gggaaatatt tagaagtcta
    13261 tcattgactt ctttaaaatc atttacagat tttctttcat aattaaaaga ttttactaca
    13321 tttatgtttg atatcatttc ttcaatatgt gcatttagtt ttcccaactc aacttgttgt
    13381 tgcttaaaaa aaactctagt tctctttgca ataaaattac tcacaaacat tactatcggt
    13441 attaatatta cactgcaaaa ggttaaaatt gggcttaata caaacatcat aataaaagtc
    13501 atcaatatgt ttaagattcc tgtaattaac tgtactaatg aatttgatat tcctgtactg
    13561 atattatcga catcatttgt cactctactc ataatatctc cagtagaact tttatcaaaa
    13621 aaggttattg gtagattttt aaattttaaa aataaattac ttctcatttc tctgactata
    13681 ctttgagata atctagctgt acatattccc ttagacagtt taactgaaaa ctcaattata
    13741 taaaatgaaa gtaaaataat tattcctttt aagaaaatgg attttgaata agtatcgata
    13801 tctattatgt caactacctt tccaatagta tatggtatta cagttgatat aattgaatca
    13861 attattacta gcaaaaaaat aaatctagta atcttcttat cttttttaaa gtacataaaa
    13921 attcttttaa acgttttttt aaaatcttta ggcttttctt ttttctttaa tctactgacc
    13981 ttctgatttc cagtacttaa attcaatata cttcctcctt aatttacatc ttcacttact
    14041 aaattttgca acgcaaaaat ttttttataa attggagaac taaccagtaa atcttcatgt
    14101 gtagcaaatc caaccacctc accctcatcc aagactaaaa tcttatcaca gtcaattata
    14161 gaactaatct tttgtgaaat agttattaca gtacatttct tactttcctt ttctcttaaa
    14221 ttacttatat actcttttat attctttctt ataattgtct ctgtattaat atccattgca
    14281 ctaaaacaat catcaagaat taatatttgt ggttttttgg ctatagctct agctatagta
    14341 attctttgtc tctgtcctcc tgataaattt ttaccccttt ggaataaaac ttcatcataa
    14401 gtattttcga gttttgttat aaattgattt gcatttgcta tttcacatga ttttacaaca
    14461 tcctctttgc atatatctct atttcccata actacattct ccaatatact ttttccaaac
    14521 acactactat tttgaaatac tacacctatt cttgccctta aactttcttc agaaatactg
    14581 tatatactac gtcctccaat cttaatatca cctttatcaa ttatatataa tttttgaatt
    14641 aaatttacta aagaagtctt tcctgaacca gtttgaccaa tcacaccaat attttgacct
    14701 tcttctattt taaaggatac gttttttaat attttttcat ctgtattttt gttatacgaa
    14761 aaatctacat tttcaaaaat tatatcacag tttacaaact ttaaatcttt ttctccaaca
    14821 tctaaacttt cttctctctc aattacttct tcattctcat caaccaaaac ctcacttatt
    14881 ctctcataag atgcttttgc cctaacaaaa acattaaaaa catgtgaaat cattataaga
    14941 gatgttaata ttctagtcat atagtttaca taagcaacta taatcccaat tttaatttct
    15001 cctgaataaa ttaaatgcct tccaatcagt aatattatta ttatggaaac attaatgatt
    15061 attgtaattg tcggtctaaa aaatgacatc atagttgtag cttttctact atttttaaaa
    15121 agttcatcat ttaactcttc aaagctttgg ttctcataat cgtatctaca aaatagttta
    15181 actactttta ttccacttaa aaactgtctt aattttgaat ttactttatc tgtactttct
    15241 tgtacatttt taaataatct ataccctatc ttcatattaa gcaatattat aataaatata
    15301 attaaaactg caattagaat aattattgac atctttaaat ttaattttat agctataatt
    15361 aaacttccta tacacattat tggtgacttt ataaaaactc tcatcattcc attaacgaat
    15421 tgttgcattt gattgatatc attagtaatt cttgttatta atgaagctct accaaattta
    15481 tctatttgtt ttaatgagta tgtatttatc ttttcaaata catcctgtct taaatcataa
    15541 gaaaaatgct gagaaactct tgccgagatt acacttctta ttattgcaaa tattgcacct
    15601 aaaaaagtta tacctatcat tatagctcct gttgatagta tgtagtctaa gttcttattt
    15661 gaaaccccta tatctattat ttttgccatt atagttggtt gagctaaatc acaaaaagct
    15721 tccaatgtta aaaaaaatac tgctgttaga aataatacta tatatttttt tatgtatttt
    15781 ttaaaatacc tcattcaatc atcccttcat agaattttct cattaccaaa agtatatcat
    15841 aatggctaat ttaattgatt atatcaatta taaatatttg atttattatt aatttttata
    15901 ccatatattt tataaattat ttgtttttat ttcctatatt tatatat
//
LOCUS       DAMJWU010000083        15716 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMJWU010000083 DAMJWU010000000
VERSION     DAMJWU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.086"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            153..1370
                     /locus_tag="PC460_002763"
     CDS             153..1370
                     /locus_tag="PC460_002763"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF2601640.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1465..1845
                     /locus_tag="PC460_002764"
     CDS             1465..1845
                     /locus_tag="PC460_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2601641.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2129..2611
                     /locus_tag="PC460_002765"
     CDS             2129..2611
                     /locus_tag="PC460_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2601642.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2632..3447
                     /locus_tag="PC460_002766"
     CDS             2632..3447
                     /locus_tag="PC460_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2601643.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3500..5050
                     /locus_tag="PC460_002767"
     CDS             3500..5050
                     /locus_tag="PC460_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2601644.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGYIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSVIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5147..6499
                     /locus_tag="PC460_002768"
     CDS             5147..6499
                     /locus_tag="PC460_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2601645.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6630..7388
                     /locus_tag="PC460_002769"
     CDS             6630..7388
                     /locus_tag="PC460_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601646.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLISNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELFNLLHTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7435..8154
                     /locus_tag="PC460_002770"
     CDS             7435..8154
                     /locus_tag="PC460_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2601647.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8278..9678)
                     /locus_tag="PC460_002771"
     CDS             complement(8278..9678)
                     /locus_tag="PC460_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF2601648.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9682..10590)
                     /locus_tag="PC460_002772"
     CDS             complement(9682..10590)
                     /locus_tag="PC460_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2601649.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPASEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKMIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10851..11498
                     /locus_tag="PC460_002773"
     CDS             10851..11498
                     /locus_tag="PC460_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2601650.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11822..14461
                     /locus_tag="PC460_002774"
     CDS             11822..14461
                     /locus_tag="PC460_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2601651.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14519..15622)
                     /locus_tag="PC460_002775"
     CDS             complement(14519..15622)
                     /locus_tag="PC460_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF2601652.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIHLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT         5921 a         1896 c         2738 g         5161 t
ORIGIN      
        1 atgtttattg tatgcaattg ttgtattttt aggttttttg tgattaatta aaaaattata
       61 aaaatcgttt acatgttgat ttttaaaaaa aaaagatata gaattatata gaaagtaata
      121 aacaaaaatt ggatttacgg gagtggaatg aaatgatgaa tgttacctta acagatgtaa
      181 aagaggctag agagaccatt aagaacatag taaaaagaac tgacttactt gaaagtgtta
      241 aacttagcga aaaaacagga gcaaatgtgt tctataaatg tgagaatctt caaaaaacag
      301 gttcttttaa attaagagga gcttgtaaca aaatagcgag tttaacagat gaagaaaagg
      361 caaatggagt tatagcttca agtgcaggga atcatgctca aggagttgca ttgggagcaa
      421 aaatgactgg tataaaatct acaatagtaa tgccagcaac tgcgccttta gcaaaagtta
      481 gtgcaactaa aggttatgga gcagaagtag ttttaaatgg tgcggtttat gatgatgcat
      541 atgcaaaagc tgttgaaata caaaaagaaa caggagcaac tttcttacat ccattcaatg
      601 ataagtatgt tatagcagga caaggaacta tatctcttga aattttcgaa caattagaca
      661 ataaagttga tactatacta tgcccagttg gtggaggagg aatcatctct ggtgttgctg
      721 ttgcagcaaa agcattaaat cctaatgtaa aaatagtcgg tgttcaaact gcaaatatac
      781 cttcaatgaa agagtcaata aaaaatggaa aagtaacaac agcatttaat gatactacaa
      841 tagctgatgg tatagctgtt aaaactccag gagatttaac ttttgaaata ataaatgaat
      901 tggttgatga aatagtagtg gttgaagaga cagaaattgc agaatcaata ttatttatga
      961 tggaaagtca aaaaatagtt tcagaaggtg ctggtgctgt atgtacagct gctatattaa
     1021 gtggtaagta tgtaccagca aaagatgaaa atgtagtttg tataatatct ggtggtaata
     1081 tagacataaa tacgttatat agaataatag gtgttgcttt agctaaagaa ggaagaagat
     1141 attcatttag tacaattatg gaagataagc caggaaactt tgctgagtta actagaataa
     1201 taagtgagaa tggtggaaac attttaagtg ctaatcaagg taaattatca gcaggggaag
     1261 ctctaggaaa acaaagtgcg gaatttatat tagagacaat agattatgac catatagcta
     1321 gaataaagaa agctatagaa gaaaaaggtt ttaaaattat agaattataa taaaaagtgt
     1381 atataatata caagaaaaat agtataatag tgtatgaaga tacattttat gtgtattaga
     1441 ttaacgaatt aggaggattt taaaatgaaa catgaagtaa tacatactaa tgatgcacca
     1501 gcagctttag gaccatattc tcaagctata aaagctggaa acttgttatt tgtatctgga
     1561 caagttcctc ttgtaccaga gactatggaa gtagttgaag gagatgtaca agctcaaact
     1621 gctcaatctt taaaaaatct taaagctata ttagctgaaa gtggagctga tttttcaaat
     1681 gtagttaaga ctacagtatt tataaaagat atgaatgaat ttggggctat aaacgaagtt
     1741 tatgctgaat attttggaga aaataaacca gcaagagctt gtgtagaagt tgctagatta
     1801 ccaaaagacg ttaaagttga aatagaagta atagctgtat tataaaaata caatattttt
     1861 aatttgaaaa ggctgaaaaa ctatattcta gtgattcagc ctttttataa aaaaataaat
     1921 cacaataaag aacatgataa atattgtcga attaaaaaat aaaattaaaa taaatattgc
     1981 aatatttttg agaaggtttt ataatgagta tataatttga aacgtgtaac tattaaatta
     2041 ggcatgagtt cttggatatt tttatcctga ggattcatgc cttttttgta ttgaaattca
     2101 aaaatctatt taataaggag gaagtactgt gttaaatttt ttcaagaaaa ataaaagcta
     2161 taagttacat gcagtagtga gtggtaacag cattaatatt gagaaagtaa acgattctgt
     2221 gttttcaaaa aagttaatgg gagatggagt tgcaattatt cctaatagta atgttgttgt
     2281 agccccttgt aatggtaaag taactgtgct tacagaatca aagcatgcct ttggtatggt
     2341 ctcagatgag ggtgttgaaa tattagtcca cataggaatt gatacagtta gtttacaggg
     2401 tgaggggttt aaaaatgaag tgtctcaagg agatactgtt aaaaaaggta gtccaataat
     2461 aagttttgaa agagaaaaaa taaattctca gggaattgat tgtacaacaa taataattgt
     2521 attaaatcat agcgaatttt cagaaataaa ttgcatggta gaaaatgaag ttgttgcagg
     2581 gcaagatact gttattgaaa ttatgaaata atagaaaggg attaattcaa tatgagagta
     2641 gtaaaaatct ttaataataa tgcattatct acagtaacta atgataggaa agaagctatt
     2701 ttactaggtc ttggtatagg ttttaataaa cgtccaggag ataaagttaa tgaagataaa
     2761 atagaaaaga tttattatgt tcaagaccac atgcaaacaa aatttttaga attattaaaa
     2821 aatgttagtc cagaagtgat ggatgcttca caacaaatta taagtcttat accaggaaat
     2881 gagggaaaat ttaataataa agggatatta tctttagtgg aacatattag ttttgcaata
     2941 gatagaatga aaaataatgt ttttttacct aatttaatgc ttttggatat aaagatgatg
     3001 tattcaaaag agtttgaatt aggagtaaaa gctttagaaa ttatatatag agtatgtcat
     3061 atccatttgc ctgaagatga agcaggttac atagctttac attttgtcaa tttacaatca
     3121 aatgataatt tggcatatga tactttaaaa tttgtaaaag gtagtattga tttgattaaa
     3181 gaatgttatg gattagaatt agacgaatcg agtttaagta cattacgttt tagaactcat
     3241 cttaaatttc ttgctcaaag aatttttaag aatgaaattt gtcaggatga taaaatgatt
     3301 gaaatgtatg attatttaat taacaatcat cctaaaaata aagaatattt agaaaagtta
     3361 aatgtatata ttgaaaagga gtttaggtat aaattagata aacctgaaaa aatttatcta
     3421 ctactacacc taacaaaaat cttataagaa tgagatgaat tagtaataga aatatatata
     3481 attttagggg gggataagat tgaaagaaaa actacttgga ggactggaaa catttgcaaa
     3541 ggcaatggtt cagccattaa tgtatttatc gagtgcagga atattaatgg tggtaggtgt
     3601 actacttaca aatgatatgt tattaggagt acttccattt tttaaatggg agccaattca
     3661 aatatttgga agcctaattt ataactgtat aatggctatt attaataatc ttgctgttat
     3721 atttgctata ggtattcctg ctgcactagc aaaaaaagag aagcacaaag cagcgttaat
     3781 aggatttatg agttatataa tataccttac agcttccaac actatgttaa catcactagg
     3841 tcaactagca aaagaaagca agatgcttgg actgataggg actggacaag ctactatttt
     3901 aggtattcaa gttgtagata caagtgtatt tggtggaatt attttagggt atatagctgg
     3961 atatgtattt aatcgtacaa gcaacaaaac atttaaagga gctttacaga tatactcggg
     4021 agcaaatttt agttttatat gcatgttctt tgtatccatt ttaattggag ttgtgagtaa
     4081 ctttatatgg ccattagctc aacaaggaat agcttctctt gcaaatgtca tcaaagatac
     4141 aggtacattt ggtttattct tatatggttt tttagagaga atattagtac caactggact
     4201 tcatcattta atttatacac catttcagtt ttctgattta ggaggagtgc ttaaagttgg
     4261 tgcagataca gtttcaggag cctatgctat agttatgaca gaaatgaatt taccagttga
     4321 gcgtttctca gatagtatat actacatggc aactggattt acaaagatgt ttggttatat
     4381 aggtatagga gctgccttta tttatactgc atataagcaa aataagaaga aggttaaggc
     4441 aacgattatt ccactaataa ttacggcagt attagcctct gtaacagaac cattagactt
     4501 tatgtttgta ttctgtgcac cacttttatt ctttgtacat gcagttatag ctggtctttt
     4561 tatagcttta ttaaaaatat ttgatgttac tgcattatgt ggagggaact tattagtatc
     4621 agttataatg aatactacag taggcgtaga aaaaacacac tggccagtaa tgatgatatt
     4681 aggacttgca caaattattg tatattttgt agtatttagt tttttaatta agaaatttaa
     4741 ttataaaact ccaggtcgtg aagatgagag tacagctaca ggcgaagaac aatcaaaaca
     4801 acttaattta gatgcaggga tagaaaatat aattgatggg ttaggcggaa aagaaaatat
     4861 taatacagta gaaaattgca tcactagatt acgtgttaat gtaaaagatg aaagtaagat
     4921 taatgaagat ataattaatt taacgccaaa tagcgggatt gtaaggaaag gaaaagatat
     4981 acaaataatt tttggacttc atgtgcatga ggtaagacgt gcagtagaag attttttaga
     5041 agcttattaa agatatatat ttatatagca agggtaaata acagctttaa gtgctgttta
     5101 ctatttatta aagatacata taatttatag aggaggaata attattatga ttattactat
     5161 tgttggtgga ggaagcacat ttactccagg tattgttaag tcaattgcat taagagaaaa
     5221 agagttagag cttgaggaaa ttcgtttatt tgatattgat aaaaaacgtc aagataaagt
     5281 agctgtagtt gtcaactgga tactaaaaga agatgttaaa acagatgtta aactaactgt
     5341 aactaattgt gaaaaagaag cgtatacaga tgctacattt atatttgcac aaatgcgtgt
     5401 tggtaaatat gaaatgcgtg agcaggatga aaaaattcca ttgaaatatg gttgtgtagg
     5461 tcaagaaact tgtggttgtg gagggatggc atacggactg cgtacaatac aaccaatgat
     5521 aaaggttatt gatgatgtag agaaatatgc taaaaaagat tattggatat taaattactc
     5581 taatcctgct gcaatcgttt ctgaagcttg tcgtaagtta agaccaaatg caaggattat
     5641 aaatatctgt gatatgccaa tagctattat agatatgata gcggggtcat taaatatcaa
     5701 tgacgttcat aatatacgct atgattattt tggtctaaat cattttggct ggtttacttc
     5761 aatagattat aaacatagag acttaatgga agagattaag gaatacataa aagaaaataa
     5821 aatattatta ccaaaatctt atatagaaaa cttaaataaa agtcgtagta caggaaatcg
     5881 ccatgctgta agtagctgga tacatgtttg ggaatctgtt tatacaatgt tagaaagctt
     5941 cccaaaatat ctgccaaaca cttatttaac atactatctt ttaagtaaag aaacagtgga
     6001 acattcaaac ataaatcgta ctcgtgcaaa tgaggttatg gaaagccgtg aaaaagactt
     6061 atttgaaggt gtgagacatt acttagaaac tggagaaatt tctgaaaaag cattttatgc
     6121 aggttcacat ggagattgga ttagtgattt ggcagtttca attaaaaatg atacacgtag
     6181 tcgtttttta gttataactg aaaatcgtgg agctatacca aacatgccat atgatgcaat
     6241 ggtagaaatt ccagcatata ttggtaaaga tggtccagaa gttattgctc gaggtccaat
     6301 acctctattt caacaaggat taatgatgca acaacttaac agtgaaaaat tgatagtaga
     6361 agcttatata gaaaatagtt atgaaaaagc tttacaagca ttcacattaa ataaaactat
     6421 tccaagtatg aatgttgcta aagaaatttt ggatgaaatg atagaagtta ataaggaata
     6481 ctggccagaa ttaagatagt aggataaaag aaagggtttt tatttaatca ttagatatgt
     6541 tttcagagtc ttttatcaaa atgtattcac aaacatatgg caatatatta aaataaagag
     6601 tgatattttt agaagaatgg ggtgatgcaa tgaacattat aaataaaatt ttttggcaag
     6661 aagataggat tggcatgatt acaaataatt tagaggcaga tacacatagc cactgtatgt
     6721 tacaactgtt cttagggata gaagacagca ttgaaataac agttaatgaa aaattagtta
     6781 aatgtaattg tatcatagta gataaaaata tctctcattc attttcagca agaaaaaaag
     6841 tttattactc tgctattatt gaacctacat ctatatatgc agaacagctt acctcaaaaa
     6901 tgaatgattt tggctactgg atatgtgaca atgatggatt agagaaatta cgtcaacaag
     6961 gaactttttt gatatctaac tctagtaaag aacaatattt aagatttatg gaaatgctta
     7021 ataactattt gaatatacct ataactctca aacattatga tgacagaatt actgaattgt
     7081 tcaatttatt acatacttgt aattgtgata atcatactat ttcaagtttt gcagataaag
     7141 tgtctctatc agcaagtaga ttatcacatc tgtttaaaga acaaataggg atacctttaa
     7201 aaagctatat cttatttcat caaatggagt gtgcttttag agaattactt tctggaaaaa
     7261 atgttaccga agcttctatg actgctggat tcgatactcc atcacacttt gcagggacag
     7321 ttaagcgaat gatgggaatg cctgtttctt tatctttgaa agatagcgag tttttgaaag
     7381 tatattaaat attctcgtta tataatgaag ttgtactaag aaaggggctt tgttatgaat
     7441 aaagaatatt tagaagaaac aaaaatgtta aattataatg aaccacaact taagcttttg
     7501 gtatcatcaa agaattggtt ggaattagat gattttcata aaataaaatc tatttatgag
     7561 tttgtacaga atgatatttt atttggatat aatgcttctg atatgttaag cgcaacacag
     7621 gtattaaatg atggtatggg acaatgcaat acaaaagcta ctcttttaat ggcattatta
     7681 cgtgcagtga atatcccttg taggcttcat gcttttgatg taacaaaaga ttttcaacga
     7741 ggagctacat ccaaattgat ttcactatta gcaccaaaat atattcttca tacttgggta
     7801 gaggtatttt atcaagatag gtggattgct ctggagggtg tcataactga taaaaaatat
     7861 ttagaagcaa ttcagaagaa attttttaat catggtggaa cgtttaaaaa gtatgcaata
     7921 gctacaaatg atttgaaaaa tacatctatt gattgggatg gtaaagatac ttttatccaa
     7981 aaagaagcta tcgtgtatga ttatggtatt tttccatcac cagacgtatt tttttctact
     8041 cattcacaac atatgtcaaa attaaaaaat ttcatatatg tacatcttat cagaaaaata
     8101 atgacaaaaa atgtatgtaa ggctagaaac aattatattg ataaaaatga atgagtgttt
     8161 aagaggtaac tatataggaa atatatagaa taattaaaac tgtactggtt aaaatgaata
     8221 aaaaggagtt atcacagagc aaacttaaat tgttcatagg ataactcctt ttctgtatta
     8281 acttctaata atttgatgtc tttttataaa acatatagat acaattaaac cagtaaatgc
     8341 aagagtagct gctattgaaa atgcaacatc tattccatga atgttagaca gcatgtttga
     8401 actcatatga gctttcttag tagcacttgt cattatagta ataagtatag cagaacctat
     8461 agctccagat atctgacgga gtgtgttatt aattgctgtg gcatgagata taagttctct
     8521 atctaatgtt ttaattcccc atgtagttaa tggcattaaa accattgaaa taccaatcat
     8581 acgaatacaa tacatcaatg acacatgaat taaagatgta tctctgccta aaaaagcgaa
     8641 ggataaagtt cctaaaaaca aacatcctgt tcctaatatt gatagagcat gaggtccata
     8701 tttatctaaa tgacgtcctg caattggatt gagtacaacc atcaatatag caccaggtaa
     8761 cattaatgaa cctgaagata atgcagaaaa tcctcttaca gattgaacgt acagaggaat
     8821 cattaatgta gcagacatca ttgaggcata tacaatcata attaatattg tagaaataag
     8881 aaatggttta tttttaaaaa ccctcaattc taaaaaaggt tctttagatt ttaattgacg
     8941 caatgtaaat aaaattaatg acataattcc tattaatatt ggtaaatatg tggcaatatt
     9001 agtccaccca taattacctt gattagtaaa tccaattaat aaaccaccaa aacctagaga
     9061 tgatagtact attgaaggta tatctaattt tagtttttga gtttccccaa catttcttaa
     9121 taaaataaat gatagtataa catctaatat agcaataggg gaaagaaaca gaaataaact
     9181 atgccaacca aatgaatcta ctaaccatcc tgataaagtt ggacctattg ctggagcaaa
     9241 tcctactgta ataccaacta aactcatagc tgcaccacgt ttttcctcag ggcataaata
     9301 gagtataata acttgtaaaa gtggcattag agaaccagaa cccattgcct gcaacacacg
     9361 agcaatcaac atagttgaaa aattattaga aaaaagtgca ataatacatc ctatagaaaa
     9421 gatagacatt gatgtaataa ataactttct agtactaaat cgatttatta aataagctgt
     9481 tgtgggtaca ataatacctg caataagtag ataaattgtt gttaaccact gaccaacatc
     9541 agcagtaatt ttaaaatctg acattatatt aggtaatgct gttgacaata gtgtttgact
     9601 gagagaactt ataaaagcac cagtcattac aattgctatt attaaatttc cttttttatt
     9661 gttactttgg gtttccataa tttataacgt ctcctttttt aattgatttt taatgctgtt
     9721 caataaaagt acttttggtc ttaaagtgtc ttctttttct aaatatgcaa tcattgcatc
     9781 cataattaaa gaatcatatt gttgattttt tccctcgacg tataattttt ttattgcttc
     9841 tagtaattta ggattttcaa ggagagaagt atcagttttg attagttctt ctatatcttg
     9901 tatcatttta aaaaatagcg atttattatg aagggaaaag taaacttcat cttcttgttt
     9961 atgtaaaaac attgattttt caattaaagg atttagatta tccatatttt ctacaactaa
    10021 atctagaatt tgtatagtaa atttggcaat aaagtataca tttatttctc ttttttgtac
    10081 atgaataatg tatataaact gatgaattag agcatccaat aagaaaacta aatcaaatat
    10141 aattttttct attttatcac catagttatc aaataagctt aatttgtatt cattcaataa
    10201 gttagctcgt gattttgttt gcaatttact tgtagcctta cgttgctctt cagaaaaatt
    10261 ttctcttata taggtattta tggtagttct agaaaaaata tttttccata cagcaattat
    10321 ctttctgttt aacttttctg atgctggaat gtcattattt tcatttatgg aatgtataat
    10381 ttctaaaaac ttttcatcat aataattgat tatttcgcag ataaaacttt ccttagtttc
    10441 aaaatgcttg taaaaagtag ctttagacat tttacactct tttacaacat ccgatattga
    10501 agtattttga aaaccctttt ggtcaaaaat tttttgtgca atagttataa tgagcctttg
    10561 tttttctgtc actttattat ctcctttcac gcaaaaacgt ttagtactat agagtaccta
    10621 caagttttaa taaatatatt atacatcatt tggaaaaaaa atcaatatat agactgtaat
    10681 atgaaatttt tgaaaaattt gttgttattt ttagtcgtat aaaaaagaaa cttaagagtt
    10741 tcttaagaat tgtttaaaga agttgcaata aattagtggt atgattatag tatgaaagag
    10801 aatgtcttga aatttttctg tatcaaattc caattatgga ggtgtagtat atgaaaaaga
    10861 aaatttgtgt agcaataatt tttgctctat ttatagtttt tgtagaaatg tttattgata
    10921 gctctagcga aggaagtaaa attatgacag ctgaattaag taaatacagg aaaggcgtaa
    10981 atgtattatc aataaacaat attatagtag cgaataaaaa atatagtttg cccaaagatt
    11041 attctcctca ggaaagcagt gaagcgagag atgcttttta taaaatgaat aaagatgctc
    11101 aaaagtcagg attaaattta aaagctttta gtacatatag aagttatgaa tatcaagata
    11161 gattatttaa gtcatatgta aaagaacatg gagaaaaaga agcaaataga ttttcagcta
    11221 aacctggaga gagtgagcat caaactggac ttgcttttga tattggagga gatgaccagt
    11281 cttgttgggc aaataaaaag ttcaacaata caaaagaagc taagtggttg tatgaaaatg
    11341 catataaata tggttttata cttagatatc cagaaggtaa agagaatata acaggatata
    11401 tgtatgaatc ttggcactat agatatgttg gaactgaaca tagcaaaaac tttgcaatga
    11461 ataatttgac tttagaagaa tatttacata taaattaaga gatggtttgt taaaattagg
    11521 ttaaaataaa aaataaccta gactattagc tgtgtaaagt gatataatgt aaatacaatt
    11581 tagataacta ctcgaagggg agtagcttac acgaaagtgg taattaatcg tcaagacagt
    11641 agaaatactc ggttaattag caagggattc cttgatagca agaccttcaa aataagcatt
    11701 aggtatgttt gttttggggt ctttttttat tcaaggaatt tttatttaaa aaaatttaaa
    11761 tttcaagtat acataagagc ccccaaacat aaaataaatt tattgaagag gagggcaaca
    11821 aatgtcttac aaaagtactt atcaagaagt aattaaaaat ttagactcaa gtttatctgg
    11881 tttgagtaaa gaaagagcag aagagttgtt agaaaaaaat ggagctaatg aattaaaaga
    11941 agcagataag gttcctactt ataagctttt cctagaaagt tttaaagacc cattagtaat
    12001 aattctattg atagctgctt tagtacaaat atttttaggt gaaacagtgg agtctataat
    12061 aatttttgca gttattatta taaactctgt tctgggtgtt gtacaaacaa aaaaagcaga
    12121 aagttcatta gaaagtttga aaaatttatc tgcaccaaac gcaaaggtaa taagagacaa
    12181 taaaaaaatg acaattccag caaaaaatct agtagttgga gatattgtat tcttagaagc
    12241 tggcgattac ataccagcag atggaaggct tatagaagca caatctctca aggttgtaga
    12301 agggatgtta actggagaat cagaaccagt tttaaagcat acagaaaaaa taagtgaaga
    12361 tgtaagttta ggtgaccaga aaaatatggt atttagtggt gctgtagttg tttatggtag
    12421 aggttctttt gtagtgactg gaacaggtat ggatactgaa atgggtaaaa tagctgggct
    12481 tttagaaagt gctgaaaata aacttacacc attgcaacaa aaaatagagg attttagtaa
    12541 aaaacttggt gtaggtattg taatacttgc tcttttaata tttataattc aagtagcaag
    12601 aaattacttt atggctggaa atggagaaat gacacaaatt attttagatt cattcatgtt
    12661 ctctgtagca gtagcagtag cagctatacc agaagctcta tcatcaatag tcactatagt
    12721 attggctgtt ggaacaaata gtatggcaaa aaaacaagcc ataataagaa aattaccagc
    12781 agtagaaact ttaggttcaa caagtgtaat atgtactgat aaaacaggta ctcttacaca
    12841 aaataaaatg acagtagttg attattatat gtatggaact aataatgatg agttagataa
    12901 agaaaaagta aactattctt atcatgctaa attattgact atggtttcta cattgtgtaa
    12961 tgactctcat ataactagtg atggtaaaga agttggtgac ccaacagaag tagctttaat
    13021 aaattattca agtaaaaatg atttagatta tgataaatta agaagtaagt atatcagaat
    13081 aaatgaaata ccatttgact cagacagaaa gcttatgtct actgtaaata gtatatatgg
    13141 agattatata atgtttacaa aaggtgctcc agatattgtg tttagtagat gtaaatatgc
    13201 tttgaaaaat ggttcaaaag tagatataac agatgagata atagaagaat ataaacataa
    13261 aaatgaagag ttttcaaata gagcacttag agtattagct tttgcaataa aagatgtccc
    13321 agaagaagat tttatacctt caattgatga cgaacatgac atgacattgg ttggaataat
    13381 ggcaatgata gacccaccaa gagaagaagt aatggatgca gtaaaagagg caaagagtgc
    13441 tggtataaaa actgttatga taactggtga ccataaaaca acagcagcag caattgcaaa
    13501 agagattggt ataatggaag aaggagactt ggctcttact ggaaaagagc ttgatgcttt
    13561 gagtgaagaa caactatatg aaaaactaga aaatataagt gtatatgcca gagtctcgcc
    13621 agaaaataaa attcgtatag taaaagcatg gcaacataag aataatataa cagcaatgac
    13681 tggtgatggt gttaatgatg cacctgcatt aaaacaagca gatataggaa tcggtatggg
    13741 aagtggtact gatgtagcaa aagatgcttc tgctatggta ttggttgatg ataactttgc
    13801 aagtatagta aatgctgttg aagttggtag aacagtatac agtaatataa taaaatcaat
    13861 tacttatcta tttgcaggaa acttaggagc tatagtatcc atagtatttg cagtgtttgc
    13921 aggttggtca aatccattta cagctttaca acttttgttt ataaacttag taaatgactc
    13981 tttaccagca attgcattag ggtttgaaaa gtcagaaaaa aatataatga ataaacctcc
    14041 tagacacccg gatgaaagta tactagcagg aggaacattg aaggcagttg ctataagagg
    14101 ttgtataata ggtgtagtta caataattgc acaatatata ggattacaag tatctccaga
    14161 acttggagta gcaatggcat tttcaacatt gatattagct agaataatgc aaacattacc
    14221 tgcacgttct aataatcaaa cagtaataaa attaggtttt ttcagcaata aatacgttat
    14281 aggagcagtt ttagtctgtc ttgggcttta ctctttaaca ctaataccag tgctaagagg
    14341 aatattctct ataccaacaa cgtttggatt gtaccagttg gggatttgcc tagggttagc
    14401 attaatttca tcaatattaa tggaacttaa caaattaata aatagaagtg aagtaaaata
    14461 atcaaaaaat taaagggcat attgatttta acaatatgcc ttttataaat agtctaattt
    14521 attctatatt atcgattatt tggaatgctt ttataacttc aggtaattct ttttcttcag
    14581 gcattctaaa tcttatgaaa tttttatcaa gtccagtaaa atcacttcca gatataactc
    14641 ttatttttaa tttagcaaac tcttgttgta aatctatgtc cttgttttta tgttcaactg
    14701 tcataattga tacagtatca ctagtttcag ctatattaag gtttttccat ggaattaata
    14761 attgattttt tatatctttt gttttttctt ttaattcttc tataaattga acatcatcta
    14821 ataaatttgc tgcaatacta cgagatattt cagacacttc gtaaggagta gaaatctttt
    14881 taatagggct aacaagttgt tctggaagaa ctgcatatcc tgctcttagt cctgctaaac
    14941 cgaatccttt tgaaaatgtt tttaaagcaa ttacattatc ataattattt agaagcttga
    15001 cagctgaatt ttcttttggc atgtattcac cataagcttc atctaccata actgctatat
    15061 catatttagc tgcttccttt actatatttt caattgatga taaatgtata atttgtcctg
    15121 taggattgtt tggattgtct atgtaaatta ctttatattc tggatttatt ttttcaataa
    15181 attctttttc attaaacttg aaattatcct ctttctttaa aagaacataa tcataagttg
    15241 caccatgcat ttttatatct gtctcatatt ctgagaactg aggtgaatat cctaaaactt
    15301 tatctccctt ttcaataaat agtctattta aaaggtaaat aacatgtata gaaccatctg
    15361 ccaaacaaat tctgtcagta tctaatgcta taaaatcttt ccaataatcg actatattat
    15421 cttttaaagc aattgaatga ggatatcctc ttatcatatc aaattttaaa gcattaaaag
    15481 cttctacagc tttgtttgag taagacacgg tatttatacc ttcaccacag tctatctcga
    15541 atacatcttc tgaatgtcca gattctattg cataactttt tttcaactgc atttcaatag
    15601 ctttgttaat ttttagtttc atactttatc actccttatt tagtcaataa taaatgttat
    15661 aaaagttgct agatttaaaa tattatgaaa atatataata gtaaaaaatg aatata
//
LOCUS       DAMJWU010000084        15625 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMJWU010000084 DAMJWU010000000
VERSION     DAMJWU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.087"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(39..899)
                     /locus_tag="PC460_002776"
     CDS             complement(39..899)
                     /locus_tag="PC460_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2601653.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1216..2544)
                     /locus_tag="PC460_002777"
     CDS             complement(1216..2544)
                     /locus_tag="PC460_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2601654.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2692..3021)
                     /locus_tag="PC460_002778"
     CDS             complement(2692..3021)
                     /locus_tag="PC460_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2601655.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3045..4334)
                     /locus_tag="PC460_002779"
     CDS             complement(3045..4334)
                     /locus_tag="PC460_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2601656.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4350..4676)
                     /locus_tag="PC460_002780"
     CDS             complement(4350..4676)
                     /locus_tag="PC460_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2601657.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4811..5440)
                     /locus_tag="PC460_002781"
     CDS             complement(4811..5440)
                     /locus_tag="PC460_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601658.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5656..8313)
                     /locus_tag="PC460_002782"
     CDS             complement(5656..8313)
                     /locus_tag="PC460_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2601659.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKHYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8672..9616)
                     /locus_tag="PC460_002783"
     CDS             complement(8672..9616)
                     /locus_tag="PC460_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601660.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9609..10634)
                     /locus_tag="PC460_002784"
     CDS             complement(9609..10634)
                     /locus_tag="PC460_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601661.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10709..12277)
                     /locus_tag="PC460_002785"
     CDS             complement(10709..12277)
                     /locus_tag="PC460_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2601662.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGVFWNIADWTIE"
     gene            complement(12390..13301)
                     /locus_tag="PC460_002786"
     CDS             complement(12390..13301)
                     /locus_tag="PC460_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601663.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13285..14271)
                     /locus_tag="PC460_002787"
     CDS             complement(13285..14271)
                     /locus_tag="PC460_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601664.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14631..15032
                     /locus_tag="PC460_002788"
     CDS             14631..15032
                     /locus_tag="PC460_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2601665.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15035..15433
                     /locus_tag="PC460_002789"
     CDS             15035..15433
                     /locus_tag="PC460_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2601666.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         4925 a         2733 c         1726 g         6241 t
ORIGIN      
        1 ttatataata agtattttta ggattctgtt acataatatt atttaaatat tcttctatat
       61 ttctcttctt cttcttcagt aaacacatta aatacaactt ttaaattttt ttcaggattt
      121 tcttttagcc atagtctaac tctacttaca gcaagattag ctgcctcttt ttttggataa
      181 ccaaatacac ctgtagaaat tccacaaaaa acaatatttt ttatttcatc aatttctttt
      241 attgtattta aacatgattt ataacaatga agcagttgct tttcttgctc tttacttggc
      301 tgaccaccag atacaatagg tccaacagta tgtactacaa attttgcagg taaacaataa
      361 cctctagtta tcttagcatc tccagtatat tctaaatgcc cttgtttttt tattattttg
      421 tcacaatctt ctctaagtct aggacctgcg catgaatgta tctcattatc cacgcataga
      481 tgtaatggtt gtaaacaccc taataacttg ttatttgcag catttactat tgcatctgct
      541 cttaaattcg ttatattacc tctccatatg gcaatccctt cctctacttc ttctatatca
      601 tttacatcta ctaactctct ttcttcacat tcaatcaaca ataaattatg taacatatta
      661 agtatctctg gagcaatttc ttttggtgga tatacattca aaagtcctct aagtatatct
      721 ctaggcttaa tttcatcatt tgataaattg tatattttag aaggaacttc ttttgaaaaa
      781 tatgcaatta aagtgtttat attttttctt ctttgttcgt cagttagagg cttgatagtt
      841 ttatcaaaat cctcaaataa atttacatca tcagcataat ctctccactt catagccatc
      901 tctccattct tgtattttag acttaaaaat aattatgtat ttatgtacat atttatttat
      961 ttctataaac acctacataa atcctgttaa aagtgtaaat tttttattat acaacttctc
     1021 tggttatttc tattgattga tagtaaatta ttgtacctaa atttaatcca actaacaagt
     1081 aactaaacct actttcctaa ctgcaaaata cactatgttt gccacttgta tctcatttgt
     1141 agataaataa agtccctaca aatacattaa ctaggctatg tactaaactg taggaacttt
     1201 aaaaatatat tattcttaat taagttttgg ccaataattt ttatttgctt ctattaaatc
     1261 atctagtaat tcttttgcaa ctgttgcact aggaactgtc ttagatagca taagtgcttg
     1321 ccacaattta ttataagact tctcaatcca agcatcaact actaattttt caactgctaa
     1381 ttgttgttct attaaacctt tttggaatgt tggtattttc cctattgata atggttcata
     1441 cccgtctttt ccaactatac atggaatttc aaccatagca tcatcttgta tattttctat
     1501 agctccatta ttttgaacta tcaataacat acgctcctgt gtattaaaag ctagagctgt
     1561 agctaaatcc actataaact ctgcatgtat tcctatctta agcccactgc ccttagaatg
     1621 tccttgcttt tctatcaatg cacatgaacc aaaaacttct ttttctcttc catcaataac
     1681 ttcattggca cgagtataat ttacatcaga atgatttaca acataatctg gaaatagata
     1741 gtactttaag taactacttg gtattgtagt taaatctgtt tttactattt ctttagtaaa
     1801 taaattagtt tcaaaccaac tatctttctt gtcatttttc attccttctt caccatttat
     1861 aaaaccatat tcttttatat gttctactaa ttttggtaaa agttcatttc cttttttatc
     1921 tttaattgaa gtataccaac caaagtgatt taatccaaag tatcttatat ccatatgttt
     1981 acgagatttg agtcctgcaa tccttgctat attatgttcc atacctattg gcatatcaca
     2041 aatatttatt accctagaat taggtctcaa ccttctaaca gcttccgcta ttattgatgc
     2101 tggattagaa taatttaaca tccaacaatt tggagaatac ttttccatat aatctatgtt
     2161 ttcaagaaca ccttctatag acctcattcc ataagctatt ccacctggtc cacaagtttc
     2221 ttgaccaact accccatgtt ttagaggtat cttttcatct agttctctca tttcaagttt
     2281 tccaactctt atatgagcta aacaaaaatc tacatctgta taagcctctt tagggcatgt
     2341 tgttgctaaa tactctatat tagggtcctt ttcctttatt agtatttcac aagctttagc
     2401 taccttattt tgtctttctt catcattatc atataatttg attgctctta atggtaacct
     2461 atctaagtta tctagcaaca ttaatatgat ttctggggta tatgtacttc ctcctccagc
     2521 tattactact gaaaattttt ccataataat atctcctttt ctatttaaaa atttactgta
     2581 tttataaata aatttataaa tactagtact ttaaagtaaa agggagttca tttattaaat
     2641 ctattgattt aataaactat agccaaactt caagcactat ttacaagaaa tttaattctt
     2701 attaattctt gggtccatca tctcaaatat atctacaagt tcagatacta aagaatgagc
     2761 aaataatgca tttgaataat ggtcttcaga gtgcacaact aaaaaatcag tcccactatc
     2821 ttcactattc atagcatttg atagtaattc taaatgatgc tttgatgctg tttgtatttt
     2881 ctctgaggct gaatttaaaa ggtgttttgc tttattgtag tcaccttttt ttatttcctt
     2941 aagagacatc attacatcac ttttagcatc tcctgcttct gatattatac taaatacttt
     3001 ttcttccatt tcaggtacca tatccttctc cttctaatga cttgttaaaa atcaaagcta
     3061 tcatcatcta atgttgtagc taattctgca tttttattta attcttcctt ctccattatc
     3121 ttaaagaatg gataatatat caaggtcata agtattatac ttgcaaatac taatacgatt
     3181 gccttccaat ctaatgtcat caaaaatgct gcaacccctg gaggtaaaaa gcctggtggt
     3241 tctgcaatag gtaatcctac taatcctact tttaatgcca taaaattaag tgctccaacc
     3301 aatacaggtc caaatacaaa tggtacaaac atatatgggt tcataactat tggtgttcca
     3361 aatattaccg gctcatttat tccaaatagt gatggtaaaa tcacaacttt tcccatacta
     3421 ttaaaactct tacacttact tcttgacata aggtacacta atcccaaagt tattcctgaa
     3481 ccagttactt gcaagaaacc atagtaaaat cctattgtaa acacatattt tgggtctcct
     3541 cctgctgcta tcagtgttgc attttctgcc atatatgtag ctgctattgg catccatata
     3601 cttgaagtta ttgctgctcc atgaagccca aagaaccata ataattgagt tataaatagt
     3661 attatagtta ctgcaaaaac attatctaca ctgcttatag ctggtgctaa gaaattcatt
     3721 ataatagctg gtagtggttg acctgagaca tttttagtta ttgctgctat tgctgtaaat
     3781 aacattatac atactatcat tggagttaaa tttctaaaac ttttatttac tacactaggt
     3841 acttcttttg gaaatctaat tacgagattt ttatcattca taaacttagt aatatcaact
     3901 actaaaagtg ctactaatat acttacaaat aaaccttgtg catctaaata acttgtatct
     3961 aatacaccat ctactacttg tccacataca acaagttgtg caacaactgc tgtcatgcct
     4021 atttctacat gattaagatt atatttttta gctagtgcct gagctataaa aaatgctgaa
     4081 tatatagata ataaactcat agtaaattta ttagggataa gtaatgtatc catattttca
     4141 gataataatg tttttgcact tccttcaggt aacatattgt ttaatgccat aaataacata
     4201 cttaaagaac caacaattat tattggggta atgcttatca taccttcttt tatagcttga
     4261 agatgccttt gtttttcaat cttttgtgct attggaagaa gaaatcttct taaattattt
     4321 tgaagtgctt ccatttttat tccccccaat tatctatttt tatataattc gattgccgac
     4381 ttcataacag aaggagcatc catacgacca taatgcttag tatctattaa accaactgct
     4441 attttgtaat tcttacaagc ttcaactaca tcatcttcct taaacctaat ttgtggtgct
     4501 acaagtacta catctgtatc attagcttca atgtatgatt ttaaattttc aaatgatgtt
     4561 gcctctatat ttatagtttt agtttttaac ttttcatgct cactacaaaa acttttcatc
     4621 ttttccacaa gtacacctgt tgaagctcca tttgcacata ctaataaaat attcataatt
     4681 accctcctac aatatctaac ctcttttatt ttctaatttt ttatcagcca attcttactc
     4741 taattcctat cagtttttac tttaattctt attattttat tttaattctt attaatattt
     4801 actctaattc ttagtactgc caatgattaa acttgaaagc cgtatctata acttcttctt
     4861 gcttatattt ttctaatatt tttaagtatc tttctttata atcattatct acttcttttt
     4921 ctggtattac tatactagct tcatttaaaa agtggaatga atcccttccc atttctaatc
     4981 ctctatatgt atgcttgtca agtgccacgt ccataacttc tggaacttta cccattgcaa
     5041 atccttttat aattatattt tttaataaat cacttgatct atctttctca catgcacaaa
     5101 gatatcttat tgcatgtatg aaaaataaag gtctgtctcc atctgcatat gaatattcct
     5161 ttcttacttg atttaaatta tttatgatta ttacagctga tggatccccc attcctatat
     5221 cttcaactga tattgtaagt atccttctcc atagcttatc ttctaattct ggtgatgata
     5281 tatatagttc ataagcaaat tcacaagctt cttccaccat atttcttctt atagcttttt
     5341 gaaatgacga tattatttcg tctgcactaa atccgtttct tgtagtcatt cttgcccatg
     5401 ggtcatttgc ctttttatct cttaatattt tatcttgcat attatttact cctttttatt
     5461 taaattttaa ttatttatat tactcattta ataatataag caagtattat gccagttttt
     5521 ttagacactt taaaacactg ttatttcaat catttttatc atcaaaactt agatactttg
     5581 tctaagtata tttatatgtg tctaatatta atattatttt agactaaaaa aaaattacca
     5641 aataagtttt tatactcatt ggtaatcttt ttcgattatt tctgcatatt ctaatattaa
     5701 taatatttga tagcactctt tttcattaat ttctatatta tattttttat ctaattgaga
     5761 tactatatct tttattttgc ttagactctt gtgataatca agtgtttttt ctttatcaag
     5821 cttttcaatt tctctagtat ttccaatcaa cccatctacc atgaaagcta aatgcatcat
     5881 aagtccaatt actttatcgt atttcttttc tatattaaat actaaaaaca attcatcaga
     5941 cacatatttt atatctttaa atattctatt ataattagag ctttttaagg atgtctttaa
     6001 accttcaact acatcatcat aacctatact tatctttata atatttttta atttattgat
     6061 attttcatcc ttatctatat cttttttgct aatatagtca attcctctaa tattaattct
     6121 ttgttcattg attactgcaa caatattact actttcttta agtttcgtaa tatttaaact
     6181 taaatctttt tctgttttat atgggacaga tataatatct attccatcta agtacctact
     6241 catcttttct tctaatattt ttcttgtttt attatctaca ccttctgaaa catgtgatgc
     6301 tataataatt gttttagaca gattttcttt attttgaatt tcattagttc caaaatagtg
     6361 ctttctcgtg ttagtgatag agttgtatac gtcatttaaa ggtaatccca tattggcctt
     6421 ctgaactgct tctaacacag ttaatgtcgt caccatagga attactctta ctccacactt
     6481 tttttctttc tttataattt catcaaagga gtttaatgaa cccatatctg taaatagtaa
     6541 tacatcttta ttgtctatat tattaattaa atttttaaaa tctacaataa cttcatcata
     6601 gcttttatct aacttcatat cgaaagaaat agcactgcac tttccaataa tatcaccaac
     6661 tgcttttacc atactacttg ctgtagtttc tccatgcata gcaactataa tattaatgtt
     6721 attcactatt ttattttgtt catcttgttt agcttgtact atgaataatg taatatatgc
     6781 tacagtttct atagataaaa ataaatcaaa ttcattttca attattccaa ctatttttaa
     6841 tgctgtttta aattcacttc tgttatttct tctaatttga ttcaaatcta tgttgtttgt
     6901 agagctactt ttatccaata cgctttttat ataaattgcc attgacttaa atattgattc
     6961 acttatgtct atttcttcat cttctttaat aatttgtctt attgattcac acatgctttt
     7021 aaacttaaca ttattaaata gagaagcatc tgctatattc atttctacag atttattaag
     7081 attagtattt actgcattaa taatatttat atcattgtag ttaataactt cttcttctcc
     7141 taatgacgta aatacaaaat attctattcc atgtgttata acttcatctg ctttaataga
     7201 cttctctcta taatcatcac tcacttcatt tatagattta tcaaaatatt ttttctctat
     7261 ataaactggt tctttattat ctcttttata tcttagatat ccatttgcaa cagctaattt
     7321 tatatcattt tgaagttgac ctacattatt tttacaatca taaagtaata gtgatttaat
     7381 tatttcttta tgtactttta ttgaaataga aatacttttt gattcttcct tgaaaaaatt
     7441 tttgactaat tccattcttt caatcattgt cctatctctc aatgatggta tttctatttt
     7501 cattggtatt cttctattga atgtatctaa tagtgttgat gtcggatttt ctgtagtagc
     7561 acatattatt aataccttag actctctttc tttcgttgca tctcctagtc ttttaaatgt
     7621 tcccttatct ataaaagtaa acaacatttc ttgaccttct gctggtagtc tatgaatttc
     7681 atctaaaaat agtatccctc catttgcaat ttccaaaaga ccttttctgg aagattctgc
     7741 acctgtataa gctccttgtt ctaccccaaa aatatgtccc ataagtaatt gtgcattatt
     7801 agcataatca gcacaattaa atgttacaaa aggagcttta ttttttctaa ttccactatc
     7861 tatagaataa tcgtacatat atctagcaat catactcttt cctactcctg tttctccata
     7921 gataatagta tgaagtccat ttgtaggata gagcattgcg accttgcatt tttctattat
     7981 aggtcttaaa ctatgttctt tacctattag tgtttcaaaa ctatcttttt ttattttgtt
     8041 ttctactttt gttccttttt ttatgccaag caactcactt tgtgtatcaa gcaattcatt
     8101 cttgttaagg ttaagatttg cagggatata atacacaggt cttcctttta tcttcgtaac
     8161 cataccctca ttgaataatt ggtttaaata tgtacttata ttgtttcttg caattcctgt
     8221 cttttctgat aattggttag ctgtaatccc tttttcattt tccctatatg atgattctaa
     8281 aactttcatt actttatctt ttttatatat cattacaagc tcccctacat tatagtattt
     8341 tattaactaa aacccctata cacactttat tttttaataa actcttattc tttaactaag
     8401 gttataattt atcaaacttt ccttacttta aattatagaa aattattgta gaaaaaataa
     8461 gattattgct cattttttct acattactat ttttaatttt atagtacttt cttaacaaca
     8521 tactttactt gtttttaatt ttattatatt tgaatttatt atatttgaaa agtagaaata
     8581 aaaaacccta ttttggagta gttttaaaat ttctccaaaa atagggtttt agtttgtatt
     8641 tcaaataaat acctgttcaa gtatttttat actaaacaac ttttaaattt tgttgatatt
     8701 tatttatttc tgatttagaa gaatatacaa aatgtccatc tgaaacctca atccaactta
     8761 atgacttttc atttgaacaa tcatatccat tagaattata ctctattcta tttcttgact
     8821 tagcatatct agggtcagga acaggaattg cagaaagtag tgattttgta tatggatgaa
     8881 taggattatt atacaattca tttgaatctg ccaattctac taattttcca ttatacataa
     8941 ctcctatacg gtcactaata tgtctcacca tagataaatc atgagctata aataaacaag
     9001 ttaaattctt ttcttgttgc aattttttaa ataaatttac tatttgtgct tgtatagaca
     9061 catccaaaga agctataggc tcatcagcta ttataaattc tgggtctact gaaagagctc
     9121 gtgctatgcc aatacgctgt ctttgtccac ctgaaaaatc agatggatac ttatttgcat
     9181 aacttctatc aagtcctact agctttagca tctcatatac tttattcatt ctatctgttt
     9241 tatttttaca gataccttga atttttagtg gctctgaaat tatatctgca attgtcatac
     9301 gtggatttaa tgatgaagta gaatcttgaa aaataatctg catacttttg tttacatctt
     9361 tttttataaa tttgtacgtt tttttatctg atatgcaatt tcctttgtaa attacttctc
     9421 caccagtaga ttcatgtagt cttattatag ttttaccagt tgtagatttt cctgaacccg
     9481 actctcctac caatccaaat atctcaccct tatatatatc aaaacttata tcatcaactg
     9541 cttttacagt agtacttttg tccaaatgaa aatattgctt taagtttttt atttccagta
     9601 ttttttctct attcattaga aatcaccctt ccacaattta ctctaactgg tacatctact
     9661 tcaggtgcat ctgggtgcaa taaccaagtt gctgcactat gtgtatcatt aattttaaac
     9721 attggtggct ctttttcata atctatctta agtgcatttt tattccttat tgcaaaagca
     9781 tctccttttg gtgggttcaa taaatttgga ggcattccag gtatattata taaatagtca
     9841 tcctgcgaat ctaaagtagg taatgaaccc aacaaccccc aagtatatgg atgtctaggg
     9901 tcataaaata tatcctctac agttcctatt tctacaatct ttcctgcata cataactgct
     9961 attctatcag ctatagttgc aactactcct aaatcatgag ttataaatat tattgacaaa
    10021 cctattttat gttgtaaatc ctttattaaa tcaattattt gagcttgtat agttacatct
    10081 aaagcagttg taggttcatc agcaatcaat acatctggat tacaagcaag tgctatagct
    10141 attactatac gttgtctcat tccacctgaa aaatgatgtg gaaattgttt aaatcttttc
    10201 tcaggattat caattccaac caattcaata agctctatgg ctctcttttt tgcctcactt
    10261 ttacttatac cttgatgaat gattacagct tctgcaattt gctttcctat tgatatagtt
    10321 ggattgagag atgtcatagg gtcttgaaat atcattgata tattctttcc tcttatatct
    10381 tccatctctt tttcagactt cttaaccaaa tcacttgatt taagtaaaac ctctccattt
    10441 ttaatatatc cattgtatgg gagtatcctc atcaaactct tacacagtac agatttccca
    10501 caaccagatt ctccaactat agccaatgtc tcaccttttt ttagattaaa agatacattt
    10561 cttacagcct gaacttctcc ttcttctact ttaaaactta cagatagatt attaacctcc
    10621 aataaatgtt ccaaaaacca tcaaccccta tgctattcaa accttaggga tatattttta
    10681 tccctaaggt ttctaattaa tttcagtatt attcaattgt ccagtcagct atgttccaga
    10741 atacacctac accatgatga cctaaaactg tatcaggtgt taatcctttt atgtttggtt
    10801 ttccaacata tattgcatct atataagcaa taaatgtata aggcatatcc ttagtcattt
    10861 ctacttggaa ttgtttatat aactttaatt tttcatcttt atcttctgtt tctcttgctt
    10921 tttgaagtat tttatctata gtaggattag agtaagcact gtaatttgca cctttatctg
    10981 ttccaaatac tttatatgta tggtcatctg ggtcaaatgg acttccccaa cctattaaat
    11041 gagcatcttg attagcccaa tctgtttcag ttacaacaac agctttagca tcaacaccaa
    11101 tttcttttaa ctgttgtgca catattttag ccatatcaac tctaacttgg tcactctcac
    11161 ctgctgttat ctcgaaagct aacttagtac cttctttttc atagattcca tcagaaccta
    11221 atttccaacc tagcttttca atttcttgtt tagctttttc tggattatat tcaaactttt
    11281 ctatatcagg attattatat ggtcccattt gtaaaggtga atatgctggt acaccatgtc
    11341 ctaataatac actgtcaact attgcttttc tatctattgc atagcttaaa gcatttggta
    11401 atcctttagc ttttttatct ttaaagaatt tagaattaaa attgtataat atacctctat
    11461 agtcggctgt tttcattata tttactttaa aattcttttc gtctttttca aagttagaca
    11521 tatctttagg agttatttga gctaggtcta attctcctga ttttaattgc attgccttag
    11581 ccttatcatc tggaacaatc ttaaagacta ctttatctaa tcctggttct tttacaaaat
    11641 aatcactatt cttaacaagt gttatacttt gacctttgtc ccatttttca agtttaaatg
    11701 gtccagtacc tattggtttt tgattaaatt catcagtagc tatatcctta ccctccaaag
    11761 catgttttgg taatactcct actgttaaat aatcaagcat tgctgtattt ggagctttta
    11821 aagtaatttt tatagtatta tcattaacca catcaatttt tgttatatct tcataatttg
    11881 atgctatctc agaagcatta tctggattca ttatagtttc tattgtaaac ttaacatcat
    11941 ttgctgtaaa tttctctcca tcatgccact taacatcatc tcttaaatta aaagtatatg
    12001 tatttgttgc ctcatcaaat ttccagtcct ttgctagaca aggaaccacc ttattatttt
    12061 catcatgagc tgttaatcca ttaaatatta acgaatttat ttctccatgt tcatatagtg
    12121 caggatttat acttgtatag tcattacttc cataaacaag cacctttcca tctttaggcg
    12181 tatctgcctt cttatctttg tctcctccac tagaacaacc tgccattaga ctaagtatca
    12241 ttactaaact taaaactttt aacttcttta atttcatgat gctccccctt ttgcttttta
    12301 taaaatattt ttcaaagctt tgaccccatt tatttaaata gatatttcaa atacctcagt
    12361 atttgcttat ttaatactca aatgctgatt tataaattac tagatttttt attattattc
    12421 tttcttatat aattacctat atttgttata cacaccaatg tggtaaccaa aaatataccc
    12481 ggaatcagta taatccacca tctatttgaa agtaatgctt cctctgacaa agataacata
    12541 cttccccaag aaacaatttc tattggtaaa ccaattccta aaaagctaag tgtcgattca
    12601 gtaccaatag ctgcgcctat gtttgttaca accatgaaca ttattgatgc cacaaaattt
    12661 ggaagtaaat gttgctttaa tacatagaag aatcctcctc ccatgctctt agctgctagt
    12721 atgtattcgc tattttttat ttgtctaact tctgttctca ctatcttaga tatattcatc
    12781 caacttgtta tccctattac tatggacata gatactggat ttgaatttcc aagtatagct
    12841 tgtacaaata ttataattaa tattgaaggt atactaagta tgatttctgt aaacctcatc
    12901 attgcatcat ctacgagttc agatgccgaa ccacttatac taccataaac aattcctata
    12961 acagttgata ttacagtgga aaatagtcct ataaataaag atatttttcc tccataccat
    13021 atcatagaat atatatctct acccattgag tctgttccaa ataaaaaagt tttatttgga
    13081 gctaaattag aacttgctaa atccatgtat gttggttcat gtgtcattat aaaagatgag
    13141 aatacacttc caatgactat gattgataaa ataataattg atatatatgg taattcttta
    13201 atttttttag ataaggtttt ctttgtttct atatgttcat cttctgctac taaaatataa
    13261 ttttctccta caatttcaaa atcactatat tgtggcttca ttagtatcac ctctatcata
    13321 tttcattcta gggtctattt tattgtttat aatttgagct aacatattcc caaatactac
    13381 taaagcccct gttattaagc ataaaactaa taacatatta taatcatgat actttgcact
    13441 ttcaaaacaa agtgttccta acccaggata agaaaaaact ttttcaacta cataagttcc
    13501 tcctagtata tgaggtatcg aaatggccat tatactaata tatgatggca ttatatttct
    13561 caggcaatgc ttaaaaacaa tagttctatt atttaaacct tttgctttac ataaaagcac
    13621 ataatcttct cttatttcct ctagtaattt atttcttatc atatatgtgt aataccatag
    13681 atggcttaat ataagtactg taagaggtaa gattagatgt gatatcctac ttaatatact
    13741 ggactcttct cccattgaat aagcaccact acttggaagt atacttaagt ttatactaaa
    13801 tataagtatg agtactaagg caacccaaaa tgatggaata cagttggtta ttgttcctaa
    13861 tttgcatatg attctatcaa tgagcttatc ttcatgtaat gtacaaaata ttcctaacaa
    13921 taaggctagc acaaaggtaa gtatatatcc tgaaccacct aatattatag tgtttatata
    13981 gacatcattt ataacagaag taacattttg tttatattta aatgaaattc caaattcccc
    14041 ttttgatgca tttgaaatcc attttatata ttgactgtat attggttcat ccaaaccaag
    14101 ttttttcata gcattttctt tttcttgtgt actcattctt tctactccat ccccatagta
    14161 agagattaat gggtctcctg gagcaagtct tgccatgtaa aaaacaataa aagataataa
    14221 aaacattact aaaataaact gcaacaactt tttcagtata tcctttaaca aaccccaacc
    14281 cccctttgtt gcaataaaaa aaaccacata aaagcaaacg taaagtaacg ttaaaacctt
    14341 cgtggttcca ttatcattaa acaaaattaa attttctata tttatctatg agttaatttt
    14401 agcatcattt attaactgtg taaacaaact ttttaaaaat tgttaaaatt tcatcaaatt
    14461 attttattta tacctaaaat aaaattttta tattctattc ttcattattt tctataaaat
    14521 tataaaattt taggttgact atataagaaa agtatattat attagtttgt atgcaaacca
    14581 tttaagaggt gattttattg aaaagtaatt atgactgttt tagaatagcc atgctcttaa
    14641 aagaacttta ttctaaaact atgtacacag tagaagaaaa ttttaaagaa aatggattaa
    14701 cacaccaaca gattatagtt ataaaactga tagctcataa tcaagaactt acaatatcac
    14761 aactatgtga tgaaatgtct ttagctaaag gtacggtttc aggcataatc agcagattag
    14821 aacagattgg atatatagaa aaattcaaga aatctaatga taaaagaaat acatacgtta
    14881 agtttacaac aacaggattt gaatttgcca caaattttaa aattaaaatg caagaaagtt
    14941 ttgatgatat ttttaaaaat tgtgatgaaa acgaattgag tgacttagta aaaaatctta
    15001 gaaatatatt ggcaaaagta aaggagagat aattatgaat tataaattaa tacttgcaat
    15061 agtatttata acatcagctt taattttcta cactataggt gtatttggag aaagaaaggc
    15121 aaaaatttta aaaaagaaac atgtaattat attttggctt ggttttatat ttgatacttt
    15181 aggaacattt acaatgagta acatagccaa cagccatact tttgaagtaa aatctgcttt
    15241 atcacaaaac cttcatagta ttacaggtct tcttgcaatt gtgcttatgc tttttcatgc
    15301 aagttgggct acttttgtcc tatacaaaga tgatgaggaa aagaaaaaat tctttcataa
    15361 gtttagtata gttgtatgga ctatatggtt agttccatat tttataggta tgtttattgg
    15421 tatggctgga tagtaaaata aaaagtgtgt aagaaatact gtaggtaaat ttgtataaga
    15481 caaatttatc tacatccttc tttcacactt ttacatattt atttataata ctccattatt
    15541 tctttcatgt ttcttaatac ctatatagaa tatttaatta tttctatagt attttattat
    15601 tttctcacac tttttcatat atata
//
LOCUS       DAMJWU010000085        15456 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMJWU010000085 DAMJWU010000000
VERSION     DAMJWU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.088"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            164..1555
                     /locus_tag="PC460_002790"
     CDS             164..1555
                     /locus_tag="PC460_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2601667.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1808..3601
                     /locus_tag="PC460_002791"
     CDS             1808..3601
                     /locus_tag="PC460_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2601668.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3682..5064)
                     /locus_tag="PC460_002792"
     CDS             complement(3682..5064)
                     /locus_tag="PC460_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2601669.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5322..5669
                     /locus_tag="PC460_002793"
     CDS             5322..5669
                     /locus_tag="PC460_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2601670.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5687..6721
                     /locus_tag="PC460_002794"
     CDS             5687..6721
                     /locus_tag="PC460_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601671.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6770..7819)
                     /locus_tag="PC460_002795"
     CDS             complement(6770..7819)
                     /locus_tag="PC460_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2601672.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8094..9629
                     /locus_tag="PC460_002796"
     CDS             8094..9629
                     /locus_tag="PC460_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601673.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9814..10500)
                     /locus_tag="PC460_002797"
     CDS             complement(9814..10500)
                     /locus_tag="PC460_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF2601674.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10625..12007
                     /locus_tag="PC460_002798"
     CDS             10625..12007
                     /locus_tag="PC460_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2601675.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12409..13677
                     /gene="eam"
                     /locus_tag="PC460_002799"
     CDS             12409..13677
                     /gene="eam"
                     /locus_tag="PC460_002799"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF2601676.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13770..14126)
                     /locus_tag="PC460_002800"
     CDS             complement(13770..14126)
                     /locus_tag="PC460_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601677.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14277..15371)
                     /locus_tag="PC460_002801"
     CDS             complement(14277..15371)
                     /locus_tag="PC460_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2601678.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5980 a         1916 c         2489 g         5071 t
ORIGIN      
        1 aataatattt ttcataattt taaaaataat aataaaaaaa tgaaaataca ggcgataatc
       61 tattgagtaa acatagaaaa gatgatatta taagtatgtg ctaaaatggt taaagcaata
      121 ttaatattaa atttaactat aaaacaaaag ggggcgtttt tttatgtcaa gtacagaaag
      181 cgtatctaaa aaaagtggtc atccgaagag tttttggctt atttgcatga caattttgtg
      241 ggaaagattt agttattatg gtttaacagc tttattggta ttatttttta cagcaagtgt
      301 agctcaaggt ggtttaggtc tttctactca agaagcaaca tccatgtttg gcttatttac
      361 aggtcttata tatcttacac cattagtcgg tggctggtta gcagataaag ttttaggtca
      421 acaaaaatgt atttctatag gtgcactttt aatagcaata ggagatttcg tactatttgc
      481 ttcatctaca tctattgcaa tggtttgggt aggtctatca ataattattt taggaaatgg
      541 tttttttaaa tcaagttgct caaatatagt tggggatata tatcctaaaa ctgatgtgtc
      601 aagaaaagat gcagcataca gtttattcta tatggcagta aatgtaggag catttatagc
      661 tccaataata tgtggattgc tttctgataa tgtatttgca gtaagaggag caagtggaga
      721 tatagtatct tatggatata agatggcatt tttaatttgt ggtataggca tgatgttagg
      781 tatgattata tttacacttc ttgcaccaaa attattaaat gaagtcggaa aatatcctgt
      841 gactaaaggt aataaaggtg aaaaaattga gtatggacca cttactaagt tggagaaaaa
      901 taggataata gcaatggtta ttttattttt atttgtggtc ttattctgga cagcatacta
      961 tcaaacacaa agttctttta tgatttatgt aagggataat gtaaatagaa ctctatttgg
     1021 atttgaaatg ccagtagcat ggcttacatc actaaatgct atattatgtg tggctttatg
     1081 cccagtatta gctaccctat gggttaaatt atctcatacc aaaagaggtg atttctctat
     1141 tccagttaaa atgggattag gaatgataat aatgggtgtt gggtttatag ttatggtatt
     1201 tgctacatta gctaatggtc cagatggaat ggtaaaggct agtatattgt ttatagtatt
     1261 gacttatata tttacaacag taggtgaatt attcttatct cctataggac ttgcaatgtt
     1321 caataaatta gctccagcta agtatgctac gttgtctatg ggagcctggt atctaacaag
     1381 tttttttgca agtttattat caggaaagat tgctggtttt acagccacac ttggtttttt
     1441 acagatattt ggaggaatag caacagtttt aataatattt ggtttaatat tattgggaat
     1501 aagaaatgta ttaacaagat taatggctat ggatttacta acaaaagaaa aataattcat
     1561 gacgaattat tgtaaataaa tgaattactt taaataaagg aagttacatt taaatataaa
     1621 attaaatgta atttcctctt tgattatgaa aaaaagcttt acacaaatag atattataat
     1681 taaaattata attgtgataa ttagagtgta taagatttat ataaattata caaaaaatat
     1741 ttatgtaata aactttacat acttatatat ttagttattc aaactattat ttcgaggagg
     1801 tcttgttgtg aatataaaag atagattaag tggattaaga aaatttatgg aagaaaaaaa
     1861 tatagatgcc tatatgattc catcttctga taatcaccaa agtgaatatg taggagatta
     1921 cttcaaatca agagaattta tatcagggtt taatggttca gcagggacgg ttattgtgac
     1981 taaagacgaa gctgggttat ggacagatgg aagatatttt atacaggcag aatctcaatt
     2041 agaaggaagt acaataaaat tattcaaaat gggacaagaa ggttgcccaa ctacagatga
     2101 atatttatat aaaaatattc ctgaaggagg aactttagga tttgatggta gagttatatc
     2161 tgctagagaa ggagctacat tagctgaaaa attatctaaa aaaggtataa agatagaata
     2221 tcagtatgat ttaatagata gtatatggcc agatagacct gctttatcag atagtaaagc
     2281 atttttatta gatgttaaat attgtggaga aagttttagc tcaaaattag ctagattaag
     2341 agaaaaaatg agtgaaaaag gtacttctac tcatgtaata actactcttg atgatatagc
     2401 atggttattc aatataagag gtggagatgt taaatataat ccagttgtac tttcttatgc
     2461 agtaataact ttaaaagaag tttatctatt tgttgatgaa agtaagttaa atgaagaaat
     2521 actaaatgaa ctagctaagg aaaatgttca aataaagcca tataatgatg tgtatgaatt
     2581 tgtaaaaaat atagacaaaa ctgaaaaagt tttattagat ggaactaagc ttagttatac
     2641 aatttacaat aatatacctt gtgaagttga gaaagttgat gagttcaatc cagtaatgtt
     2701 ttttaaagct caaaaaaatg aggtagaatt agaaaatata agaaattcac atgtaaaaga
     2761 tggtgtagct ttcactaaat ttatgtactg gttaaagaaa aatgtaggta agatggaaat
     2821 cactgaaata agtgctactc aaaaattaga ggacttaaga agagagcaag aaggtttctt
     2881 tgagccaagt ttcaatacaa ttgctgcata taaagagcat gctgcaatga tgcactatag
     2941 tgcaactcca gagtcaaatt ataaattaga ggcagaagga ttatttttag tagattcagg
     3001 tggacaatat tatgatggaa ctactgatat aactaggact acagttttag gacctataag
     3061 tgatgaatta aaactacact ttacttctgt agcaagagga atgataaatc tttctaaggc
     3121 taagttcttg catggttgta gaggatataa cttagacata ctatctagaa gttgcatgtg
     3181 gaatatggga attgattatc aatgtggaac tggacatggt ataggatttg tgctaaatgt
     3241 tcacgaagct ccaaatggat ttagatggag agtagtacct gaaagatttg atagtgcagt
     3301 tcttgaagaa ggaatggtaa ctactaatga accaggtata tatatagaag gttctcatgg
     3361 tataagaaca gagaatgaaa ttgtagttag aaaagctgaa aagaatttct atggtcaatt
     3421 tatggaattt gaagttgtta cgttagctcc tattgactta gatggtatag taccagaatt
     3481 aatgaataag gatgaaaaag attatttaaa ctggtatcat aagttagttt atgataaaat
     3541 atcacctttc ttaactgatg aagaaagaga atggttaaaa gtttacacta gagctatata
     3601 gtttgtacta gatacaataa aaatcgaggt attataatgc ctcgattttt tattgtatat
     3661 tttattaact ttaaaaaatt tttaatttga taattctact aattttttat tcattataag
     3721 tagtataatt ccaaatatca ataaaaatga tggtatcact atgaagactt gtaacatacc
     3781 aagttggtct ataatagctt gcgtatagcc agataattta tttgctgcaa atttgcctaa
     3841 aaaccaaaca cccattagga atgaaatata cttttcaggc gaaagtttat ttaccatacc
     3901 atacccgact ggagaaaagc aaatttctcc tatactttgg aatgttaaaa atccaactac
     3961 ccaaaataca cttgctttgt gaattgaatt agggtctgtt cctgtttgta taactgctac
     4021 tatcataaac aaaaatgcta tggctaatag tataaaacct aagctcattt tttttggcac
     4081 acttaaatcg ccttttttag tttgagataa ttttacccat actaaagcag agataggtcc
     4141 aagaataaca caaaggagac cattgtatga atctatccat gaagtaggaa tttgaaaatt
     4201 gccaacagta agatttatgt gttcctttgt atagagtgca atagacatag aggcttggtt
     4261 atatgctatc caaaaaaata ctgtgaaaaa agctaacacc aatattacta ttattctttt
     4321 tttctcaatc aaagttaaag gtcttgaagc tagcattttc ttatattcta gtgattttct
     4381 tatagactct ctcccgttac tagggtcatt attcaaaggt ttataaaact ttctgtttcc
     4441 aaatataaag aagaataaac ctattagaac cgtaattgat gaaaataaaa atacatatct
     4501 atatccatac atacttatag ataatccgct tttcatagca aaccattcat tagcaactaa
     4561 accgcatatt aaagggccaa ataaggtacc tagattagta gccatatacg taatagagaa
     4621 agctccatct tttcttgggt catcattggt atataagttt ccaaccatag tatgtaagtt
     4681 tcctttataa aaacctattc ctaatccaga taaaaatatc atgatgttta tatccatctt
     4741 actatttgct atgtatccta gaaaataacc aattgaaacc attagcattc caagtataat
     4801 acagtttttg ggacctaaaa atctatcact gacccatcca ccaataactg gagagaaata
     4861 agtccatgct aaaaaagatg caagcattga tgcggattct gctggtgtta accctaaacc
     4921 acctttggag acttgttcag ctaaaaacaa taataatacc aatttcatac catagtatgc
     4981 aagtcctaca aaggtaaata ttgaacagca gaaatataaa tctctaggtt gtttaagttt
     5041 ttcttgcgtt tttaaaactg acatatgtcg agaacctcca gataaatagt ttaacaaaat
     5101 tttaccatac tatatgagta tatgtttttt tcatatagac gtcaacttgt aagataattt
     5161 aattaagtcg aaatatatgt aaaaatagta tcatttttgt gtattcagta atattttttt
     5221 atgataatat atgttattat tatattaaaa tacaaacata tttatggaaa ggatatgcca
     5281 atttagtagt tagattgatt gtttggcagg tgggatgagc tatgataaga aagaaatctg
     5341 aattacaagt agaagtaaaa gaaaatttaa atggtggtat aggaaaggtt aagcttgaaa
     5401 acataataca aaatgaagag cttaaaggaa aaggaagact atttaaaaga gtagctttac
     5461 cagtaggaag ttcaattggt gtgcatgacc atacaacaga ttttgaagta tattatatac
     5521 taaagggaaa aggtaaagta tttgataatg gggaatttgt agaagtaaat gagggtgatg
     5581 tagtgtatac agctgatggc gaaaaacatt ctatagaaaa tataggagaa gaagatcttg
     5641 agtttgtagc actagtttta tatatgtaaa atatggagga ggagtcatga caggcaagat
     5701 agaagaaatt aaggacaaga taaatagatt ttgcaactcc aatttaaatc aagaatacaa
     5761 agatataagc tttaaaattt tgcaaaacct tttagataat aataaagaga taattcattc
     5821 ttctagagct gatatatggt cttcagctat actaaatata gttttagaac aaaatctttt
     5881 atacaataag aagcatccac tacatataac aaaaaaagag ttttcaaaac aaataggtgt
     5941 ttctttaaat actataaata ataaatcgtc tttaataaaa gatgtttgta gtattgactt
     6001 tttaaatcaa gatactatag ctatttctaa tgtagaattt tgggtaaaag agaccaattc
     6061 caaatcaaag tctgtagatt tagatttaga aaagaaaaaa atactatata aaagatatat
     6121 taagctatca caagatgcga atagtgatat tgaatcaatt agatatatgg aggaagctgt
     6181 aaatataggt aaaagtatga ttactaaaga agataaagaa cttggatttt ggaaaggttt
     6241 atctaccaga gcctatatga tagcacttga atcccttgct agaaagttag aaagtattaa
     6301 taatctaaag gagtccaaaa aagtattaga atatttaata gaaattaatc ctagtgatga
     6361 gcagggaata agatacaaat tattaaatgt attaattaga ttaaatgata gaacttcaat
     6421 aaataaatta tttgaacttt ataaagaaga aaaatctgct acatggatgt acagtaaagc
     6481 tctttattac tttaaaaata aaagtatgtt tttggcaaat gattctatta aggttgctag
     6541 aagtaaaaat aagtacgttg gattatattt gttagattgg cgaaatgctt ttggtagaga
     6601 atttgttaca gaggaagaaa aggcagaagc tgtctattac tatgatgaaa acatagtgat
     6661 ttggaatgaa gttaaaggtt ctatggattg gctattaaaa aaaatgttgg aattttcata
     6721 aaaaaaacta ctccttactt ttatataagt aaaaggagta gtttttaatt tatacatcta
     6781 gttttaaatc ttcaggtttt attagacctt tttttctcat aaattctgaa atcaacaaag
     6841 ttacaatagc aggaagtata aaattaagta aaagtatacc aacccacata gatatacctc
     6901 cttttccagc actttgcata gcagttactg ttccaaattg tccaaccaat ccacaagtac
     6961 ccataccaga accaatagga atattctcca ttttgaatat tgttgtagat aatggtccaa
     7021 gtaatattga agctacagta ggtggtatcc aaattttcca attttttatt atattaggta
     7081 cttgcaacat agatgtccca agaccttgtg ccacaaggcc gccaactcca ttttctctaa
     7141 agctcataac agcaaagcct accatttggc aacaacaacc tacagttgct gcaccaccag
     7201 caagaccacc taaacttaac atcatacaaa tagctgcact acttataggt aatgtaagag
     7261 ctattccaac aagtgcagag actaaagctc ccattattat aggttgcata gttgtagctt
     7321 gcattattat atttccaaat gaagtcataa aagcagaaac agctggacca ataagttcag
     7381 ctacaagtac tccaattagt attgttacag caggtgtaac caatatatct atttttgttt
     7441 cttttgaaac taattttcca acctctacag caaaaagtgt agcgacaaat gctccaacag
     7501 gtcctcctaa ttcattacca caagctccaa caagtgcaca agaaaatagt acaagtggag
     7561 gtgcttgaag tccatacgca atagccactg ctatagctgg accagtaacc tgtttagcaa
     7621 gagggcttat aacttcagta aaaagagaaa tattgaaatt atctcctaat gtattgaaaa
     7681 ttgttccaat taaaagggac gaaaaaagac cgagtgccat ataacttagt gcgtcaataa
     7741 gatacctttg aacagaaatt tcaatatttt ttcttttagc aaatgatttg aaatttcttt
     7801 ttttataatt gatttccaat ttaaaatcct cctcatataa tataagtata atttcttttt
     7861 aattaattat ataacttttc agctgacaag acaaatgtta tttcactaaa attagactat
     7921 tgtaatagta agaaattaac attaatttta gttttaaatt ttaaatttat ggtttataat
     7981 tagtataggg atattctgaa atataataat tttccagttt atcccaatat ttaacttagg
     8041 tagattgctt acaaaacaaa tgattatata ttaggctaaa aggaagtgga tagatgatat
     8101 ttgtaaaaaa taagaaatac agaaaaattg ccataattat atctattcct atattgcttg
     8161 ttgcgcagta ctaccttttt aaatttggaa tacttagacc aatggtaaag ggagtagaaa
     8221 ttgaaattgt agatggggaa tatgttaaag atatagataa atatgtagta aaattaaatg
     8281 aaacagttac tttgtctaca ggtgaatata ttaaggttcc ttcgtatgct aaaaatccaa
     8341 atatttggtt taatgtattg gataactcag ggactttaaa aatagatgga aataaaatga
     8401 cagcattaaa ggttgggtat tcttctgtgg gtatcatgaa aaattcaaga gtattaaaaa
     8461 aggctactat aaaagtagta gacccagagg tagaatcact agatatggag attaatgggg
     8521 atttaaaaca tgttggtgat agtgctacta tagaaagtac aatagaagtt gattatgata
     8581 agtttaaaaa atcatataag ccagtttata agagtagcaa tgaaaatatt ttaaaaatca
     8641 aaggaaataa agtaagtgca gttggagttg gtaaagctac aatatatgct aagtgtggaa
     8701 ataaagaaat tgagagaaca tttaggattc aagctagagt tgctgaaata aatatggatg
     8761 atataaaaat agaagttgac cagaatgaaa aattgaaacc agatataata acttctccac
     8821 ctggtttaga accaccaaaa atatattatg agttagtaga ttcaaaatta cctgtagaaa
     8881 gagcaataag tttaagtagt aatggaaatg ttgtagggct tagagaaggt aaagataggg
     8941 tgagaataac ttgtggagat aaatctaaag taataacagt aacagtagtc aaagaaacta
     9001 taacgaatga ctatatacaa aacttggttg gttcacataa aatagtaaac aataaagtta
     9061 ttattaaact tgaatgggat tatatgaaag atgtctttga ttatgatata tatcttaaag
     9121 ataagttgtc aggagaatca tttaagaaaa ttaaatctat aactataaat gaaagtgatt
     9181 taggtaagtc taacaaggta tatgcaacta tagaagtaga tatgaagggt aatcaaaata
     9241 tagattttga tatttatgtc ataggtaaaa aggattcaga tacaactaag ccaagtaatg
     9301 taataaatat taccaatgga aatggaaatt caaataatga taataatatt aaagatatga
     9361 gggtagaaaa tatatcagct aatgttgata gagataataa tgtcattaga gttaactgga
     9421 gtagtatatc tcataaggac tgtacttaca gtatatatgt aaaaaataat actaatggag
     9481 atggagaatt tactctatat caaagtgata tacagggaaa tgagcatact ataagtattc
     9541 ctgaaggaga tttaaatcta gatatttatg ttgttgcaaa ttataatgga aatagctctc
     9601 aaaatagtga tgttataaat gtaagataga tttgtataca aattgaaagt gttgagttta
     9661 tttaaatatt aaattaagaa aaaggatagt atagggtaat tatactatcc ttttttgtta
     9721 caatatatag gtttaaaatc tctagtaata taaattataa aaagtcttat ttacacaaat
     9781 ctatagatat tttttttatt aatcaatatt gtcttaaatt gaacttagtg cggcttctat
     9841 tgcatgtatt actgtcgtat caaatagtgg aatagaagtg tcattttgtt taattaaaag
     9901 acctatttct gtacatccta atataacagc ctctgcacct tgtttgccaa gcttatctat
     9961 aatagataag aaagcttttt ttgaagattc agatataatt cctaagcaaa gttcattaaa
    10021 tattgtgtca tttactatta tacggtcttc ttcatttgga attaagactt caattccatt
    10081 attaataatt acatttttat aaaaatcttg ttccattgta tatttagtac ctaataaggc
    10141 cacttttttt atttcttttt cccttaatac cgtagcagtt acatctgcta tgtgaagtaa
    10201 tggaatatgt atactttctt gtattttatc tgatacctta tgcatagtat tagtacagat
    10261 aacaataaaa tctgcccctg cctcttgtaa tttaattgca gcatcagcta aaattttggc
    10321 gcttttttcc caatttccac tagattggca ttcttcaatt tcttgaaaat ctacactgta
    10381 aagtatacat ttagaagagt gtagtccacc taatctttct tttattactg tattaataac
    10441 ttgataatat gtaatggtac tttcccaact cataccacca attaagccta tagtcttcat
    10501 attatacctc tcttatttat ttattttgaa atagctattt acaaatacaa gtatagcatc
    10561 taaaatagaa gcatacaata ataaaattgt atggagtaca attataatta agaggagtaa
    10621 ttatatgcca ataaattctt ttgaaaacta tcctatgaca tggaaaccaa agttagataa
    10681 ccgaaaacca ccaatatata aaactttagc aatgttatta gaagaagata ttaaaagagg
    10741 aaatttaaat cctggagata aattgcctcc acaacgggaa ttagcagatt ttctagattt
    10801 aaacttaagt acaataacac gagcatttaa actttgcgaa gaaaagggac taatatgtgc
    10861 aaaagtggga aagggaacat tcatttcttc tgatgtaaat gtaagtaata cattattata
    10921 tcaaacggaa tcaaaagaca taattgagtt ggggacagtt catcctccgt atgagcaaaa
    10981 tacatatatt attgacttta ttaaaaatgt actaaaacaa ccagaaatgg ataggttttt
    11041 acaatacatg tctccatctg gcacatatat gcaaaaaaag tcaattgcaa aatggataga
    11101 aagaaataat gtatacacca gtgaagaaaa tatattatta tctacaggag ggcaaaatgc
    11161 tatatgtgca actttactag gattatttaa agctggtgat agaattgcta cagattcttt
    11221 aagtttttct ggaattaagt caatagctaa aatgataggt attcaattag tcccaattcc
    11281 tcaagaaaat aatgaaatat cgattgaata tcttgaaagt tattgtaaaa atgaaaatat
    11341 aaaaggcata tatcttatac cagattatca taatccaacg acacatacta tgtctgattt
    11401 atctcgtaaa aagatagcca agatagcaaa gcaatataat ataataatta ttgaagatgc
    11461 tataaatagc atttttagag atggtataca aactcccata ttttctttag ctagtgataa
    11521 tactatatat atttttagta catctaaatt tttatgtgct ggtcttagag tagcatttgt
    11581 agttgctcca aaacgatata tagaaaattt agaaaatgca ctatataata tgaatctaat
    11641 ggtttctcca tttacagcag aaatagtaca ccgtttatta tattctccta ttatagataa
    11701 aattatagaa gagaaaagag atgcaattat agaaagaaat gaagttgctg ataaagtttt
    11761 gtcagattat aatttgattg gtgataagaa ttgcagtttt cgttggttac ttcttcctga
    11821 tgaattggat ggaaaatcat ttgaaatttc tgcaaaaaat ctaggagttc aagtatattg
    11881 tgccgaacga ttttcagtag gaaactctac agttccaaaa gcagttcgta tttgtgtaac
    11941 agctccaaaa gatgtagaag aattagaaaa agggcttaat ataataaagt cacttcttaa
    12001 gagttaaatt tattttacta ttatttaatt aaataaataa aaaggcggag aaatttatta
    12061 taataaacct ccgcttttat tatttaatga aatactttac tttttattaa aaaatttaat
    12121 tttaataatc ttgaaaatat aatcgtttta tggtaaaata attcttaaaa atagaaaaaa
    12181 ttaatcattt gttaaaaaaa atgcataatt tattgtaatt tattgacgaa ggttgaaaaa
    12241 tattcataaa tgtgcgaaat aacagaaaaa atgcaaaaat taattcaatt tcatttgtta
    12301 taaaatctat atggcaaaat taaatctgcc tatcagaatg atataaacac atcagaaagg
    12361 aggacgctat caaataatac aagtagttaa ctttgatagc aaaaaattat gaatgaacaa
    12421 actagaatat ccttagagag agctgctgaa ttaaaatcaa aaattgatga ttatattcag
    12481 gctagaaaaa cgattaacag aggtcttgaa aaagaagaag agataaataa acgaaaacag
    12541 aaaatattaa gtatcttaaa tggaactgaa gaggattgga ataactacaa atggcaatta
    12601 tcaaatagaa taacagatgt agatacttta tcaaaaatta taactctaac taaaaaagaa
    12661 aaagaatata taaaagaggt tggtactcaa tttagatggg caatatctcc atattatttg
    12721 agtcttatag acccagaaga tatatgtgac ccaataaaat tactgtctat accaacacat
    12781 atagagttgg aagatgagca agaagatttg gacccaatgg gagaagagta tacaaaccca
    12841 gcaggatgta taactagaag atacccggat agattaataa taaatgtaac aaatgagtgt
    12901 gctatgtatt gtagacactg tcagagaaga agaaatattg gacaacaaga ttctcataag
    12961 tcaaaagcta ttatccaaga atctatagac tatatcagag aaaatgaaga aataagagat
    13021 gtactagtaa ctggtggaga tgctcttaca ttaaaagatg attatttaga gtggattctt
    13081 agccaactta aagagatacc acatgttgat tatgttagat taggtactag gactcttgtt
    13141 acaatgccac aaagaattac agatgaattt tgcaatatgc taaaaaaata tcaccctgta
    13201 tatataaata ctcattttaa tcatccaatg gaaataacta aggaatctaa agaagcttgt
    13261 gaaaagttag caaatgcagg cgttccatta ggaaatcagg cagtattatt aaatggaata
    13321 aataatgata aatttgtaat gagatgttta aatcaagaat tactgaaaat aagagtaaaa
    13381 ccttattata tattccaaag taaacatgta aagggaacaa aacatttcaa tacatcagta
    13441 gatgatggtc ttgaaatcat ggagtattta agaggatata catcaggaat ggctatacca
    13501 acatatatag taaatgctcc aaaaggagga ggaaagactc ctttgcttcc tcaatacctt
    13561 gtatcaaaag gaacagatta cgttatgctt agaacatggg aaggaaaagt tataaaaatg
    13621 gaagatgaac ctgctgtaga tataaagaaa cttataaaag aacaagcaca ggattaaaaa
    13681 aatatttaaa gtttataaaa agaaacaact ttaaaataaa aagaaaatgg atgtcaaatt
    13741 tatatcaatt tgcatccatt ttttattagt tatttattat tttgttgttg attaactaat
    13801 tcaagatatc cacttgggtg gaagtaatat atctcagaag tattttcatt tacaaagtaa
    13861 ccaatatcac catctgcatc tggtaaaaat acataaccat taagattttt agcttttaat
    13921 gcttcaaaat cactagcatt tccttgatat atgtaatcaa cttcattgtt gtattttact
    13981 aaaagttctt tagcttgttc ttgagtcaat ttagtatttt ggcttacagg aacattcttt
    14041 tgagaatctg cataagaaga ttcatttact ggtgcagtta ataacaaaca agataaaact
    14101 aataaagata taagtgcttt tttcatacta aaaccctctt tcatgtttaa ttatataaga
    14161 ttcattactt aataatattg tatgtttcct ataattaaat attaccatta aagagaataa
    14221 tattacagtt acacagctgt aacttttgat tacaaaatcg taattaaatt gtatttttat
    14281 tccatttgta ttttatcatg tctacgattt acaaactcac agaagtatat ctttataacg
    14341 cccatcacag gagcagatag aatcatacca ggtataccaa agaatcctcc accaacagta
    14401 actgctagga ttgtaaagaa tgggctaaga cctagttgtc cacctacgat ttttggctct
    14461 ataaatgtaa cttcaacttg ttgaattatt attaagtaaa ttaaagaagt taaagcaatt
    14521 gttggattgg agaataaatt aattattaca actggtgcca tacctattac tggaccaaaa
    14581 tatggaacca tattcatgac acccataagt gtaccaaata atagtgcata ttcagatttt
    14641 aaaaagtaaa gaccaattcc agaaagtact ccaactataa aagaatctaa aatcttacct
    14701 gtaaagtatt taccaatatt taaatttaag ctttgacata gttctattat aaagtctcca
    14761 tattttttac ctaaggaaat ataaactact ttttttgtaa acagtaagaa tttttctttt
    14821 tctaataata tatagaaaca aattataaaa cctaaaagaa attgtactat aaattttcca
    14881 actgaaaatg tagtgctaaa tatcccacca agtgaaccaa taagcaagtt actaaattta
    14941 ggcattacac tcataatttt gtcaccatac tcttttaatg tattagggtc aactgattgt
    15001 aaactcttgc ctaagtcgaa taaaaactgc tctgttttat ctatgtatgt aggaatttga
    15061 gcaactaaat cagctaaact atttactatg gatggaactg taaataatat aaacgaaatt
    15121 agtataacta tgagtacacc ataagtaaga agtaacgaat atattctctt aaaatttaat
    15181 ttactttcta agaactttac tagtgggttg aatatatatg ctaatacaaa tgctattata
    15241 aatggtgtta gtagtgacat taataaactt atcactccaa aaaaatattt gtaattatca
    15301 ataactttaa tcaagatata tgaaacaact acagtgagta caatatttag ataaattttt
    15361 ttatcttgca aaataacaca acctttctta tatttattaa atttagatat acttaaatct
    15421 aagttaaagt taatatttat agcttattat acaata
//
LOCUS       DAMJWU010000086        15429 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMJWU010000086 DAMJWU010000000
VERSION     DAMJWU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.089"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            194..1270
                     /locus_tag="PC460_002802"
     CDS             194..1270
                     /locus_tag="PC460_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2601679.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1387..2229)
                     /locus_tag="PC460_002803"
     CDS             complement(1387..2229)
                     /locus_tag="PC460_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2601680.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            2413..2985
                     /locus_tag="PC460_002804"
     CDS             2413..2985
                     /locus_tag="PC460_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2601681.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3148..3420)
                     /locus_tag="PC460_002805"
     CDS             complement(3148..3420)
                     /locus_tag="PC460_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601682.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3547..4419)
                     /locus_tag="PC460_002806"
     CDS             complement(3547..4419)
                     /locus_tag="PC460_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2601683.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4706..7813)
                     /locus_tag="PC460_002807"
     CDS             complement(4706..7813)
                     /locus_tag="PC460_002807"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF2601684.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7906..8138)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8313..8840)
                     /locus_tag="PC460_002808"
     CDS             complement(8313..8840)
                     /locus_tag="PC460_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF2601685.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8875..9660)
                     /locus_tag="PC460_002809"
     CDS             complement(8875..9660)
                     /locus_tag="PC460_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF2601686.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9670..9927)
                     /locus_tag="PC460_002810"
     CDS             complement(9670..9927)
                     /locus_tag="PC460_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF2601687.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9946..10404)
                     /locus_tag="PC460_002811"
     CDS             complement(9946..10404)
                     /locus_tag="PC460_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF2601688.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10444..11148)
                     /locus_tag="PC460_002812"
     CDS             complement(10444..11148)
                     /locus_tag="PC460_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF2601689.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11158..11793)
                     /locus_tag="PC460_002813"
     CDS             complement(11158..11793)
                     /locus_tag="PC460_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601690.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12174..13214
                     /locus_tag="PC460_002814"
     CDS             12174..13214
                     /locus_tag="PC460_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2601691.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13677..15032)
                     /locus_tag="PC460_002815"
     CDS             complement(13677..15032)
                     /locus_tag="PC460_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF2601692.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15058..15327)
                     /locus_tag="PC460_002816"
     CDS             complement(15058..15327)
                     /locus_tag="PC460_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2601693.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         4900 a         2690 c         1792 g         6047 t
ORIGIN      
        1 atttattact tttatagaat tttcacaata aaaagtcatt ttacttccgt atttaatgca
       61 aaagtagaga ttaaacttaa tataaaaaat atataacaaa ttttttttat atttctagaa
      121 gaaaagtata ctcttttttg tttaataatg ctaaaattga tttgtattga attttgatta
      181 ggaggaaatt attatgaaag ctcaaagatt aaatgctgta cttgaacaaa tgaaaaaaga
      241 tgatatttct caaatgttag taagtgaccc tacttctata ttttatctta caggagtact
      301 aatccatcca ggcgaaagac tattagcatt atacttaaat ttaaatggca ataataaatt
      361 atttataaat gaattattcc cagtttcaga agatttaggt gttgaaatgg tttggttcaa
      421 tgatactcaa aatccagtag aaataataac tgaacatata gacaaaaatg ctactatggg
      481 tgttgataaa aactggccag ctagattctt attaaactta atagaattag gtggaggaag
      541 taagtttgta aattcatctt atataataga tactttaaga atgtgtaaag atgaagaaga
      601 aaaagagtta atgagaatag cttcaaaatt aaatgacaag gctatggaac aacttaaagc
      661 tactgtttct ggtgaactta cagaaaaaca acttgttggt aaattatcta aaatatatga
      721 agacctaggt actgatggat tttcatttga ccctattata ggttttggac ctaatggagc
      781 taatccacat ggtgaacctg gaaatgcact tgtgaaacca ggagatgcaa taatcttaga
      841 tataggttgt ataaaagata actattgtgc tgatatgaca agaactgtat tctataaaga
      901 aatacctgaa aaaggtagag aaatatttga aatagttctt gaagctaata agagagctga
      961 agctattgta aaaccaggtg ttagattctg tgatatagat gctgctgctc gtgactacat
     1021 aactgaaaag ggatatggac agtacttcac tcatagaact ggtcactcta taggacttga
     1081 agttcatgat aaaggagatg tatcttctat aaatacagat actgtacaac caggaatgat
     1141 attctctata gagccaggta tatatttacc aggtgagttc ggagttagaa tagaagattt
     1201 agtacttgtt actgaagatg gttgtgagat attaaacaaa catgacaaag aaatatgtgt
     1261 agttggttaa ttctaaaatc aatttgataa aattaaaaag ccatataaaa atgttttaaa
     1321 catttttata tggctttaat ttctcaatct atgatttatt tcaatccatt atttatattg
     1381 cagtatttat attgcagtta ttaagattgc accctttgaa gtatatgtcc ccatcactga
     1441 accaacttca cagattacaa catctttgta ttttacatcc ttttccataa cttcccttac
     1501 cttttgagcc ttttctgggc aattagcatg tcctataaat aaaacctttt cttctttatc
     1561 tttaattttt gactctacta aagtaatcag ctttttcaaa ctgtttcctt cacctcttgc
     1621 cttatctata ggagttacaa cgccatctgc aacttgtatt atcgccttaa aatttaaggt
     1681 atttataatt tttcctgcta ttggatttat tctaccaccc ttaatagcat tttctaaagt
     1741 ttcaagtgtt ccaaaaaatg gaggattatc tttatattta tttattattg aaacaatctc
     1801 ttcaatttct tttccttctt ttattaatat tgctgctttt aacactaatt gtcctattcc
     1861 aattgaccca ctaagtgaat ctataacctc aactttcttg ttatttcctt ccttttcaaa
     1921 catatctttt gctaaaagtg cactattata agtcccagat aatcctgaac ttatattaat
     1981 cactaataca ctttcttcct gacaatggta tgcttctaaa aatttatcag gagaaggaca
     2041 tgatgttttt ggtagtactt tactctcact catttttcta tagaaagttt cattatctat
     2101 ttcctttcca gatatataac tttcttctcc aaaagataca tttaaaccaa caacttctat
     2161 gttgtatttt tctattatat catcaggtaa gtcacatgca ctgtccacta tcaattttat
     2221 tttatccata ttaacctcac tataagatat tattttattt attcagtcgg tcgtacgacc
     2281 gatttaatta ttattttata tattgaatga aaaaaagtca atagatatag attctcgctt
     2341 tattgacaat aaatatccat aagcttataa tgttattaat ttgttataaa tatacatgga
     2401 aaggtaggtt ttatgaaaac aaataatatg aaacaggcta ttttatcatc tgcaaccaat
     2461 ttaatagcca aaaatggagt tcaaaatact agccttgctg atattgcaaa agatgtaaat
     2521 ataagtaaag gtactttgta ttatcactac gcatctaaag atgatattat atatgatatt
     2581 gcggatactc atttagaagt tataactagt gctatactaa actgtgtaaa aaatgtcaaa
     2641 tcaaaaaatt ctcaaataga aatggttaac ttaatcttag agaaaatatc tactatagaa
     2701 agtcgtggta gggtacatat gtatcttatt tgtgaggcaa ttacttcaaa taatgattta
     2761 aaggaaagaa taagattaaa atatattgaa tggagaacta ctcttaaatc tgaaattgtt
     2821 gaatcactag aaaaatataa tagtagcgaa aatgaacagg atgctgaatc attttctttt
     2881 ttactcatgt caatagttga tggtttagtt gtccaaagtt tattaaaaac agaaaaaata
     2941 ccttatgaaa atatagcttc ttttttagtt aatcattgga tttaaactta atacttagat
     3001 aattaataaa agtgaactat ttaagttatc tactttaaca gttcactttc ttatttattc
     3061 tcaataattt ttacttaaaa cattcaattt ttatttaaac cttcatggaa tcacaaaata
     3121 aaaatctctt ataaatttaa aatgttttta tttatgttca tgtgtatgcc catctgaatg
     3181 ttcatgacca tgtgtatgtg tatgctcatg gctatgctca tgagtacctg aaaaaccatc
     3241 atcatgatta tggtcgtgac ctacaccatt tacatgctca tgttcatgcc catgactgtg
     3301 agaatgttga tgtgaatgtt catggttatc tcctccacag catccttctc ctccagtccc
     3361 acaacaccca cttgtatgtg gattatgatg atttcttact acaaatctct ctcttaacat
     3421 aattcttata cctcctagta tattattgag tacttcatgt accccattaa ctatggtata
     3481 catctaacta ggtataatct caatacatat gtcatttttt aacatatata attaacatat
     3541 ataatcttaa agaaatttat ccttaaaacc tccacctttt acttcttcaa cgttattgac
     3601 agaaataaaa gcttcactat ccatatcata aattaaatct ttaaatttac ctatttcatt
     3661 tgcagatatt atgcaataaa tcatcttttt cttattttgt gtatatgcac cttctgcctc
     3721 caaaaaagtg actcctctac ccattttatt cattatcact ttggatattt catcatattt
     3781 atttgatatt aaaagtatag atttttttct atcaaagcaa tcttttccta tatccatagt
     3841 aaaagaaatt atatacattg taattaaagt gtacatcacc aatttagctc caaataaaaa
     3901 agctcctgca catactacaa aaaaattaat aaataaaaat gtatttttca taggtatatc
     3961 atactttctt ttcattatgg ctgctattat atcaagtcct ccagcagtag ctcttgcttt
     4021 aaaagttaac cccataccta taccagttaa tgcaccacca taaatacact gtagcatagt
     4081 atcatctatt gggaaatatt tagcaatatc ttgtgttagc cccagtgcta gagaaaatag
     4141 taacatattg acaaaactta ttagcaaaaa tttattatca aaatactttc tagctataat
     4201 aaatattggt atgttcatca agaaacttga tattccctga tttataccaa ataatttgta
     4261 taatataaca caaattccag ctactcctcc acttaataac ttggctggtg ctagatataa
     4321 atttattcct aaggttgata gaatcatacc caatataatc agacaatatt gtaaaaatat
     4381 attattatca atacttagct ttttactttc accatacatc aaaacgcacc cctttgcata
     4441 ataaataaac caccctgcat aacacacgat aaatttacaa ataaatttat cagaagtagt
     4501 ttcctaaaaa tattattcat cctcacttaa acgtttattc attaatattc tatcacatga
     4561 cataaataat ttatatgatt tatacagatt tatttattgt aaattttgga atattaaaac
     4621 attttattga atagataatt atattacata aaaaatccct atagaaatct ataaactaat
     4681 ttctataggg attttgatta actaactatt tttttataac ttttatacct gtcatatggt
     4741 catttaagtt ttgttcctct aaagatttat catctactct gtttatttct aaagctaaag
     4801 tttcagactt gatatattca ttaaaatggt caacagcttt agcaatttca tcatcaccac
     4861 agtattctat tactatatta tctaatactt caaagttatt agattttctt aattgctgta
     4921 ctttagatat aaattctcta gcataacctt cattaatcaa ctcttcagtt agcttagtat
     4981 ctagtataac aaaaagattg ttttcaacag atacattaaa tccttcctta gcagttatac
     5041 ctattaatac taaatcctta ttaaattcaa aagcttcacc atctaaatca actgttaaag
     5101 tctcaccttt ttcaagttta ttagcagttt catgagcatt taatttactt aatgctccaa
     5161 cgaataaatt catcttagaa cctaataatg gccctgcttc cttaaagttt ggttttaaac
     5221 taaaattcat atactcatct aaatctttag caaatattac ctctttaaca ttaagctctt
     5281 cttttattaa atcaactaca tcacttattg tatcttcaaa cttaccatct actaacactt
     5341 tttgtaatgg ttgacgaact tttattcttt ctacttctct tgaagctcta cctaaagtaa
     5401 ctaagttttt agctaaatcc attttttctt ctaatttagt atctactaac tcttcattac
     5461 atttaggata ttcagctaaa tgaacagata cttctcctgt taagtttcta tacatttctt
     5521 ctgacatata tggtgcaaat ggagctatag ctctacaaag accatgtaac aattcataag
     5581 ttgtattgta cactgctttt ttatcttctg ttaattcagt tgcccaaaat cttcttcttg
     5641 aacgtcttat ataccagtta gataaatcat catttataaa atcttgtatc attcttaaag
     5701 ttttatttaa ctcaaatata gctaaatttt cttctacatc tttcattaaa ttattgaact
     5761 tagataatat ccatctatca agttctggtc tatctttata ttctacaaaa aattcagttg
     5821 gatttatatc atctgtgttt gcatataaag taaagaagtt atatacattc ttcatagtac
     5881 ctataaattt actttggatt tcttttaatc catccatatc aaatttagtt ggagtccatg
     5941 gtggagaaac atatagtaaa taccatctta atgcatctgc tccatattgg tcaaataatt
     6001 ccattggatt tactgtattt cctcttgatt tactcatttt cttaccatct ttatcaagta
     6061 caaggtctgg aactaaaaca ctcttatatg gtgcttttcc taatacaaat gttgatactg
     6121 caagtaatga gtagaaccaa cctctagtct ggtctatacc ttctgatata tagtcagctg
     6181 ggaacaattg gtcaaagttt tccttatttt caaatggata gtggtgttga gcaaatggca
     6241 tagccccact atcaaaccaa cagtctataa cctctgttac tcttgtcatt ggttttccac
     6301 atttttcaca ttttaagtgt atatcatcta catatggtct atgaagttca actgtttctg
     6361 gatttacatc ttctatagct ttttcagcta attcagctct tgaaccaact gaatctgtat
     6421 gaccacattc acatctccat ataggaagtg gagttcccca atatctagtt cttgatattg
     6481 cccagtcatt tagattttct aaccagtttc caaatctctt ctctcctaca aaacttggga
     6541 accattttac accattatta ttttctatta atttatcttt aagtttagtc atctctatat
     6601 accagcttgg ttttgcatag tatactaatg gagtttgaca tctccagcag tgtggataat
     6661 tatgaactac tttttctttg ctatataatt tattttcaga agctagccat tttattattt
     6721 caacatctag cccatcttcc attacaaatc taccttccca aggagtagat gtaaatttac
     6781 cagtttcatc tactggttga ataactggta aatcatatct tttacctgtg ttgtagtcat
     6841 cttcaccaaa tgctggtgct gtatgaacta taccagttcc atcttcagta gttacataat
     6901 ctcctactgt aataaaaaat gcttttttgt caacttctac aaaaggcatt aattgttcat
     6961 attctaaacc ttctaggtct ttacctttca tttcttctaa aacttcataa tcttcaccta
     7021 gtactttatc agctaaaggt ttagatacat aatatacctc atcacctttt ttaactttta
     7081 aataatctac atcagcattt actgttaatg aaacatttga tggtaaagtc catggagttg
     7141 ttgtccatgc taagaagtat tcatcagcat cttttttctt aaatttagct ataactgtat
     7201 tagtttttac ttctttatat ccttgtgcaa cctcatgact agcaagacca gttccacatc
     7261 ttgggcaata aggaagtatt ttatgccctt catatatata tccttctttg ttgaatttgt
     7321 ttagtatcca ccatacagac tctatatagt tattatctaa agttatatat ggattatcta
     7381 aatcaacttc ataagccatt ctctcagtca ttattctcca ttgtttttca aatgagaaaa
     7441 ctgactctct acatttttgg ttaaattcag ctattccata ttcttctatt tgttgcttac
     7501 tagtaagtcc taactctttc tctacttgta tttcaactgg taatccatgt gtatcccaac
     7561 cagcttttct ctttacttga taaccagaca ttgttttata tctacaaact gaatctttaa
     7621 gcgttctaga taaaacatgg tgtactccag gatttccatt agctgttgga ggtccttcat
     7681 agaatacgaa acttgggtca tctttccctt tttctaaagt tttttcaagt atatttatgt
     7741 ctttccaata atcaaaaact tgtgcttctg cttgttttac agaactgtct accaatggct
     7801 taaatttcgc catgtaaatc accctttcct actaaatatt ataattccaa aattttttat
     7861 tctttcacat aaatttaaat actaaaaaac tcgccccctt taaaccaaag ggacgagtta
     7921 taaatcgcgt taccacccta attctatatg taaactaaat actagctaca tataacactc
     7981 taatagtata acggtactaa ccggcacagc ttagtagaat ttaatcgttc agcctgcagc
     8041 tcaagagtga tttttatctt tataaattca ttggttctca gcatacccaa ttctctgtaa
     8101 aattatctaa agataaatgt ctctatcaac gcttttaaaa atttatctgt gtttatatga
     8161 aattctataa caaataattt gaatagtcaa tatatttttg aaaaatataa taatttctat
     8221 ttttcttaca tttttatcct atttttaatc tttgttatag atttctcaaa ctcacacaat
     8281 tattctacat aaaaaataaa caaattacaa gcttattcta aagttgtagc agcttcatca
     8341 ttactataat tatacactgt tgttccttca aatgcgttta cacatttttc atctacatca
     8401 taaaaaatct catcaacact tcttatttca tcttctaaca gtgatttaaa tttatttcta
     8461 aatatcttaa attctttgac taaagcatca tactcttttc ttatctctat aacacggtta
     8521 ttagcttgtt ctataatttg tctagctttt aaatcagctt cttcaacaat tatcttagcc
     8581 tttttattag ccgcgctaca agtgtcttct gctgcacttt gagcagttat taaagtagct
     8641 tttagtgttt cttcaatatt ttcatacttg tttacttggt cttgatataa ccctaatttt
     8701 tctttaagag ctgtattttc cctacataga gattcataat cttccttgat gatatctaaa
     8761 aattcatcaa cctcttcatc tctataccct cttaaacctt ttttaaactc tttattttct
     8821 atctcaattg gagttagcat tacatttcct cccagctata atttaaatta gtccttatac
     8881 aattaatctt gcttgaactt ttatttttcc ttttttagta atatctccta tttgtgttac
     8941 cttagctctt cctttgcctc taatagaaat caaatcatca ttttttacta ttttagaagt
     9001 tgaagttatc ttttcataat tgacatggac tttttctcca tttatgtatt ttgcactatc
     9061 ttgtctagat atattgtaaa taccacttat cacacaatct aacctatctg aagatactgt
     9121 aaatgatact tctttatatt ttgaagaact attaacaata ttttctctgg aaatttcctt
     9181 tacaattaca ttatttcttg aaaccttttc taagtttaca attataaaat cgcaaatatc
     9241 actacttaca actacttgac aaaagctatc atgtataatt atatctccaa ttttttctct
     9301 ttttatacct agacccagta ttgaccctaa gtaatcttta tgagatatat ttttaaattt
     9361 gaaattccct tctatttgta aaaatctaag agtatcttct atatcttcat attccatgta
     9421 aaatggataa ataaatacag tgcttctttc agcttgttca taacctccat ccacactgta
     9481 ttttatttca tttgtagaat ttaatattgc tacagcattt ttcacttcat atggattaag
     9541 aaactccgta gacttaacat catagttttt tatacaacta ttagccttgt ctatcacttt
     9601 aaacatttta tttttcaagt ctatatcctt tatgtgattt gttagtttta atttatccat
     9661 cctatcacct tagaatataa ccaatactaa ttgtcttagg aacatcaata atactattgc
     9721 aattaaagga gaaaaatcta taggtccatt tgtatattta tacattatag accttattgg
     9781 cccttctatt ggctctgtaa gattttccaa aatatcaaaa attttacttt gtcttccatt
     9841 tggaaaccaa gtcatcaagc tttttataac tatcatccat gctattatat ctaaaagata
     9901 ataaagtgca attcttattg ttcccatata ttaaccgcct ttttactatt tattttgcca
     9961 agggaagaat gctttacttt ctaattcttt tttcatattt gcatctatat ccacattatt
    10021 aggagctagt ataaatattg atttagaaac cttttgtata cttccatcta agacataaac
    10081 tgccccattc ataaagttaa atatagattt acgaacttca gaattagtca atccttccaa
    10141 attcactata actgcccttc tttgttttag atggtctgct atagttgcag cttcatcata
    10201 aaccttaggt tcaactatta caactttcat ttggctagtt gtatgtaaat ttacaatttt
    10261 atttgctttt gcagtactaa agccactgtt catttcttct tcttgaacta tatcgtccat
    10321 gccactttca tattcatctt caatgtaatc ttcttcttca tcaactatcc aattcttaaa
    10381 tttagatatt attccatttg acattattaa cttcctcctt aaaatttatt caaatttgag
    10441 taattatttt ttattgtagt ttctttctcc aaagattgat gttccaactc taacaatggt
    10501 agccccttcc tctatagcaa tttcataatc gtgactcatt cccattgaca aaacatccat
    10561 ccctacatta gggatgttta gttcttttat ttgttttgat aaatctttaa gacctttaaa
    10621 tacacctctt atctcatctt catcttctat aaaaggtgcc attgtcatta gaccctttat
    10681 ttttatattt ttatattttt ctgacacttc tttgacaaag tctacaactt cttctctatc
    10741 aagtccatgc ttagactctt ctttagatat attcacttgt actaagcaat ctatttctct
    10801 gtttatttgt tctgcacgtt tttgaatttc ttcgcataat gcaattctat ctaaagagtg
    10861 tatcatacaa actttatcga ttatgtactt aactttattg gtttgcaaag ttcctatcag
    10921 gtgccagttt aatttatcac ctataatatc atactttcta gctaattctt ggggtttatt
    10981 ttcaccaaca gaagtgattc cttccctcat tgcctcatta actaagtcta catcaacagt
    11041 ctttgtgact gccaaaaggg taatctcttt gctgtctcta tctacttttt ttgcagcttt
    11101 atcaattttg tttcttatct cagacaaatt gtctttaata gaacccatta ttatcaccta
    11161 ctttatcctt atatttgtgt ttatactgtt aggtcttaaa attattttgt catatagatt
    11221 aacacttgta actccattta tgtcattttt ataataatcc acaaatacag aagattcatt
    11281 ctcataagtt attccttcta atatgacaaa ttttacactt tgtgattgtt catcaattac
    11341 atatacacca cgtttattgt cttttgtttt tatagaagtt ttaggtattt ttaaacattc
    11401 catctgatta tatattatat caaattcttc gacacttgta tcctgatttg caacaatttg
    11461 gttacttatt tttaatttta ccattatttt attattattt tcatatattt tgtaaacttt
    11521 tgcatctaat ttttgctcat gatattcaat tattacattt tgattttttt caaagttatt
    11581 agaatcttct ttactgatgt atgtagatag ataattactc tggtcattgg catctttttc
    11641 tatagttcct tttacatgaa tctttgcatc aactttttca ttttttaaca ccaacgtatc
    11701 tacatttttg ctaattatca ttactaatat attaaatact aaatatataa gaataccaag
    11761 tatcactaat ctggtatatt ttaccttttt caaagactct ccccctataa aattactttc
    11821 attataagta cttcttctaa aaaatgttta ttcctttttt ataagctaat attttacgtt
    11881 ctaataatta ttttttccat attaaattta ttttttatca atcttcctat acaagactgc
    11941 attatttata tataattgta ttataaagta taagtttaat ttacatttta tacgttaagc
    12001 ttactttata tgtttttata gttattttta tttttagatg caatataaaa ccaattaaga
    12061 tttaaaataa cttttgttat gttgacattt atttaggtaa tcatttatga attaggataa
    12121 cgttatcata acaaaagttt atgtcctaaa aaaaatacaa ggaggatttt cttatgtcag
    12181 aaattttatt aggaacaggt ttacttttat tggttctatg ttgtttcaca atttttagtt
    12241 acaaggcccc atatggtatg aaggctatgg gagctctagc aaacgctgca tgtgctactt
    12301 tcttagtaga ggcattcaac ctttctttac ttggtgatgt tttaggtatg gaatttttta
    12361 gaagtgttgg agctgctaat ggtagtcttg gtggagttgc tgctgctata cttgtgcctt
    12421 tagccttagg tgtatctcca gtttatgcag ttttaatagg attatcttgt ttagattttt
    12481 ctatattatc tggattcgtt gctggatact ttgtttcttt cttaattaag tttatagaga
    12541 aaaaagtgcc acctggagtg gatttagtgg ttgtaattct tctatgtgca ccgctatctc
    12601 gtcttatagc atcaaatgta gacccattag ttaatagtac actactacaa attggacaag
    12661 ttttgataga agctaccaac attagcccta taataatggg aattatacta ggaggtctaa
    12721 taacagtagt tggaacgtct ccacttagtt caatggctct tacatctata attggtctta
    12781 ctggtcttcc tatggcaata ggagcattag gtgcttttgg ttcagctttt atgaactcaa
    12841 cactatttgc aaaattgaaa ttaggttcta aaaaagatat tatatctgtt gctattgaac
    12901 cattaaccca atcagacata atttcagcta atccaatacc tatatattct actaatttct
    12961 ttggaggagc cttttctggt atcattgtag ccttgatggg tcttgtaaac atgacaccag
    13021 gtacagctac acctgtggct ggttttgcta taatgtttgc ttataatcca gttcaacaag
    13081 ttcttataac agctggttta tgtgctttat tcaatgtaat tgcaggattt ttaggttcaa
    13141 aaatattcag tacctttaaa gttcttactg ctggtgaaat acgtggtact gatgtagaag
    13201 aagaaattgc ttaaaaaaca ataagattta taaatttata tctcaagtag tagagtttta
    13261 tattgtaggc aattttatta attgcaaacg ttttattttt tatttatgtt tatgattatt
    13321 atgtgtgttg aatatttaga taaattttta tttattattt ttatttagat ttttgtttag
    13381 ttttttattt ggttgatata ttaggaataa aaattagtat agtttattac tagttagata
    13441 gcataacttt ttagatacat aaaaaatttt atattaatta gatattatta aaataaaagc
    13501 gatacatgaa tttttaaatg tccatgtatc gctttatttt tgccataatc ttaacaactg
    13561 atttttaaag tttattatct taacaaagtt cattatctta tataatattt aaatgcatag
    13621 acttctttaa tttaaccatc taaatattta attaaatatt attatatgta atataactaa
    13681 tttttaaatg aatgtatagg agctggaatc cttcctcctc tttgagtaaa taattcagat
    13741 gaatttgtat ttataggcat tactggtgct cgtcctaaaa gtcctccaaa ttctaccata
    13801 tcacctacat cacatcctgg ggctggtata attctaacgg ctgtagtctt attattaatc
    13861 atacctattg ctgcctcatc agctatcatg gctccaagcg tacctgctgt agtatttcct
    13921 ggcacagcta tcatatcaag acctacagaa caaatagcag tcattgcttc aagcttatct
    13981 atacttaaag cacctttaat aacagcatca atcatacctg catcttcaga tactggtata
    14041 aacgctccac taagtcctcc aacatgtgaa catgccatta ctccaccttt tttaacagca
    14101 tcatttaaaa gtgctaatgc tgctgtagtc ccatgagtac caacaacttc aagacccatt
    14161 tcctctaata tatttgctac cgaatcacca atagcaggtg taggagctag tgataagtct
    14221 attatcccaa atggaacatc caatctacta gaagcttcct ttgccacaag ttgtccaact
    14281 cttgtaattt taaatgcagt ctttttaact gtttctgaaa caacatcaaa aggctctccc
    14341 tttactttct ctaaagctct cttaacaaca ccagggccac ttacaccaac atttataatt
    14401 ctatcagcct caccaacacc atggaaagct cctgccataa atggattatc ttctactgca
    14461 ttacaaaata ctactaattt tgcacaacca aaaccttttg catcttttgt taattctgca
    14521 gtttctttta tgatttctcc catttctttc actgcatcca tatttatacc acatcttgta
    14581 gaacctacat ttactgaagc acatacttta ttagtacttg ctagagcttt tggaattgac
    14641 ttaattagta tcttatcacc ttttgtatat cccttttgaa ctaatgctga gaatccacca
    14701 ataaaatcta tacctaaatc ttctgcagcc ttatccaatg tctgtgcaaa ctttacatag
    14761 tcattttcat ctgttgcacc tgcaataatg gaaattggag ttactgatac ccttttattt
    14821 acaattggga taccaaattc acgctctata tcttttccaa ccttcaccaa atctttagca
    14881 gaactcataa tcttattgta tattttttct ctagctttat ctccatcagg gtctatacaa
    14941 tctaataaag aaatccccat agttatagtt cttatatcca actgttcttc atctatcatc
    15001 tttattgttt caagtatatt tcctacattc actattatca cctcccctta ctgtctacta
    15061 cagattatgc attgaattaa atatttcttc tcgctgaatt tttacatcca ctgataattt
    15121 cttacctctt tcaaccaagt cctcaaccgt tttctcaaaa gaatttaatg atttctctaa
    15181 atcaactatc ataatcatag taaaataatc ctgtaatatt ttttgtgata tatctactat
    15241 gttgatattt tgcttgtata attcatcact aattcctgcc actatacctg gtttatcctt
    15301 tccaattact gttaaaattg ctttcataat aatatcctcc cttatttttt tataaatctt
    15361 atatttttac aaattgaact atgtgttaac atatatttca tttatttgtt agtaaaccac
    15421 tttttatgt
//
LOCUS       DAMJWU010000087        15406 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMJWU010000087 DAMJWU010000000
VERSION     DAMJWU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.090"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            187..2385
                     /gene="slpA"
                     /locus_tag="PC460_002817"
     CDS             187..2385
                     /gene="slpA"
                     /locus_tag="PC460_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF2601694.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGK
                     YVDFKITYGAKAEVPAASLSADDIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNK
                     VIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASSSDVDAIT
                     LDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAV
                     EKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVT
                     IFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQT
                     VAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAP
                     LLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLK
                     VTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVV
                     DGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNA
                     EVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPA
                     PIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            2631..4973
                     /gene="secA"
                     /locus_tag="PC460_002818"
     CDS             2631..4973
                     /gene="secA"
                     /locus_tag="PC460_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2601695.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEDKIQILDDEALKNM
                     TNVFKDKLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     PVERKLQYQCDITYGTNNEYGFDYLRDNMVTQKSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKNIRDEDFEMDRKDKTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMNIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVEEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDIEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
     gene            5387..7258
                     /locus_tag="PC460_002819"
     CDS             5387..7258
                     /locus_tag="PC460_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2601696.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAEATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
     gene            7367..8074
                     /locus_tag="PC460_002820"
     CDS             7367..8074
                     /locus_tag="PC460_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2601697.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            8107..10239
                     /locus_tag="PC460_002821"
     CDS             8107..10239
                     /locus_tag="PC460_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2601698.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKSISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTDLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETIYRV
                     KTADELDVALRKADANDIITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVNMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
     gene            complement(10292..10687)
                     /locus_tag="PC460_002822"
     CDS             complement(10292..10687)
                     /locus_tag="PC460_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF2601699.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELIFLFKEFLFFVSFRLLSGIIEMIIMYIMIDLFF
                     VNDITVKVFTNIIVIVLNYLFSKMIIFRK"
     gene            10914..13325
                     /locus_tag="PC460_002823"
     CDS             10914..13325
                     /locus_tag="PC460_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HDF2601700.1"
                     /translation="MRKYKSKNLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            13622..15199
                     /locus_tag="PC460_002824"
     CDS             13622..15199
                     /locus_tag="PC460_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF2601701.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         6310 a         1750 c         2841 g         4505 t
ORIGIN      
        1 aaaaatgatg aaaaaacaat taatttgttg gaaaaaactt caattaattt tgcataaatt
       61 attgaattta tattttatat agtctatcat aagagtgtga gaaatatttg gattatatga
      121 acatggaaat ttaatgtaca taattaaaga gatgtaatta atataatgtt gggaggaatt
      181 taagaaatga ataagaaaaa tttagcaatg gcaatggcag ctgttactgt tgtaggttct
      241 gctgctccga tatttgctga tactactgtt aaagaagaag gatatacggt agtacaagac
      301 aaatatgaaa aattacttaa agaattaaag gcaaagataa aagatggaac tattacatca
      361 gttggtgttg aatttgatgg aaaaccaatt actacattag caccaaaggc agatggtagt
      421 gataaagatg caatagcaga acagttagaa actcttacta aaaaccaact taaaggttta
      481 ggtgatggaa aatatgtaga ttttaaaatt acttatggtg ctaaagcaga agttccagct
      541 gcttcattaa gtgcagatga tatacaaaaa tatgcagatc aaataaatgc ttctgagaaa
      601 atattagttg aagtagcagc aggatctgaa gcaggtatag caaaatttga ttctgttaac
      661 aataaagtta tagcaggaga tgctcctctt aaagttaaag atgcagtgaa agcgactgtt
      721 actactaatg gtagtaataa aaaagttctt acaatatcag ctgcagctgg attatcagga
      781 ttttcatatg gaacacttaa agacacaggt gcaagttcat ctgatgttga tgcaataact
      841 cttgacacta caaatgcaac aataacagaa ggtgatacta aagtattaga ttttgataat
      901 tcatttaagt ttaacgaaag tactaaaaaa gttggtagtt tagttacacc aaatacaaca
      961 aacactccag cagatcctgg tactaaaact actgttagag tgataaaagc tgttgaaaaa
     1021 acaatagatg taagttcaaa ttcaactaca aaagctaaag atttagctaa acaatatgta
     1081 ttcacagatg ttagtgatac tgatccagaa agtttaagtt atatgcttaa aaacataaat
     1141 gatggaaaag ttgctgttaa aaattctgat ggagattatg aagtaactat attcccagaa
     1201 ggaaaaagat taaatacttt atctgcttct tcagctaaaa ctattttagg agataaagat
     1261 actccagcaa aaatagtttt aaaggcttca acaacaaaga aattagcaga ctatatagat
     1321 gatttaataa cttataacaa tagttattca aatgtacaaa ctgtagcagg tagtgataga
     1381 atagagactg ctatagagct aagccgtaag tactataact ctactgacaa aaatgcttta
     1441 tatggagatc ctgtaaataa cgttgtatta gtaggttctc aagctatagt tgatggatta
     1501 gtagcatctc ctttagcagc agaaaaagat gctcctttat tgttaagttc taaagataaa
     1561 ttagattcat caacaagagc tgagataaaa agagttatgg acttaaattc ttcaacagga
     1621 ataaaaaata ataaagaagt tttcatagca ggtggagtaa attctatatc taaagacgtt
     1681 gaaaatgaac taaaagatat gggacttaaa gtaactagat tatcaggtga tgatagatat
     1741 gaaacttcat tagcgatagc tgatgaaata gatataaatg ataaagctta tgtggtaggt
     1801 ggaacaggat tagcagatgc tatgagtatt gctccagttg catctcaaat aaaagatggt
     1861 gaagctactc caatagtagt tgtagatgga aaatctgaca agttatctaa agaagctgaa
     1921 gatttcttag atgatgcaca agtagatata atcggtggag aaaatagtgt ttctgctaaa
     1981 atggaagatt atatagatga tgctactgga aaatctccag aaagaattag tggtgctgat
     2041 agacaagcaa ctaatgctga agttataaaa gaatactttg ataaagatgg agtttcaaat
     2101 tacttcttag caaaagatgg ttcaactaaa gaggatcagt tagttgatgc attagcagca
     2161 gcagcagtag ctggaaacta cggttctaag cataatgaag atggagatat aactacagat
     2221 gcttctcctg ctccaattat tttagcaact gataatttat cagctgaaca acatgtagct
     2281 gtaagtaaaa ctgctactac taatggtgct aagaacttag tacaagtagg tcaaggaata
     2341 gctgattcag ttgttagcaa attaaaagat ttattagata tgtaatataa attttatata
     2401 ttgaataaaa aaggtttctc ttttgagaaa ccttttttat tttaaacata aatttttata
     2461 aataaaaaga ggctaattag cctcttttta atgtgagaaa cacttaaaaa ataagatgta
     2521 attatttatt taagtatcat tattatacta taccttaaaa taaatgtcta ctattctata
     2581 attttaaggt ataatagtaa gtggaaaata aataaggagg taaattaaat atgtcagttt
     2641 tagatgtaat attagacaaa acagatgaat tagaaattaa aaaattaaat aatatagtag
     2701 ataaaataga tgctttagaa gataaaatac aaatactaga tgatgaagca ttaaaaaata
     2761 tgacgaatgt atttaaggat aagttaagta aaggagaaac cttagatgac atattgccag
     2821 aagcatttgc tgttgtaaga gaagtatcaa aaagaatatt aggtatgcgt caatatagag
     2881 ttcaattaat tggtgggatt gtattacatc aagggaaaat agcagagatg agaactggag
     2941 aaggaaagac tctagtagga gtagctccag tttacttgaa tgcattgact ggtaaggggg
     3001 tacatgtcat cacggttaat gattacttgg ctcaacgtga taaagaactt atgcaaccag
     3061 tatatgaatt tttaggttta acagtaggag taattattaa tgggcaacag ccagtagaac
     3121 gaaaattaca atatcaatgt gatattactt atggtacaaa caatgagtat ggattcgatt
     3181 atttaagaga taatatggta acgcaaaaat cagagaaagt tcaaagagaa ctaaactttg
     3241 ctatagttga tgaggttgat tcaatactta tagatgaagc tagaacacca cttataattg
     3301 ctggtggagg agatgaaggt actaagctgt atgaacttgc aaatgctttt ataaagaata
     3361 taagagatga agattttgaa atggatagaa aagataaaac catagcactt actgcaagtg
     3421 gtcttagtaa agctgagaaa ttttttggaa tagaaaatat aactcatata gataatatag
     3481 aaatttttca ccatgtaaat caatctctaa gagcacataa attaatgaac atagatgttg
     3541 aatatgttgt tagagataat gagatactaa ttgtagatga atttacagga agaataatgg
     3601 atggaagaag gtttactgat gggcttcatc aagcaattga agctaaagag ggagtaaaaa
     3661 taaatgatga atcaaagacc atggcaacag taacatttca aaatttcttt agattatact
     3721 ctaaattatc aggaatgaca ggtacagcaa agacagagga aagtgagttt gaagctacat
     3781 ataagattaa tgttgttcag ataccaacta ataaaccagt tataagagct gatttacatg
     3841 ataaagtatt caaaacagaa cgtgcaaagt acaatgctgt tgtagaagaa ataataagga
     3901 taaataaaac taagcaacca gtgcttgtag gaactgtttc aattgaaaaa tctgagaaat
     3961 tatctaagct acttaaaaag aaaggtgtaa agcacgaggt cttaaatgct aaacaacatg
     4021 ataaggaagc tgaaataata tctaaagctg gtaaattagg ttctgttaca attgctacta
     4081 atatggcagg tagaggaact gatatatcac ttggagctgg agataaagaa gaagaagaaa
     4141 aggtgaaaga gttaggtggc ttgtatgtta taggtactga aagacatgag acaagaagaa
     4201 ttgataatca gcttagaggg cgttcgggaa gacaaggtga ccctggaact tctagattct
     4261 ttgtaagttt agaggatgaa gtaataaaat tgtatggtgg aaaaagtata gaaaaaatat
     4321 taaagagaat gaatgctaat gaagatgacg cgttagaaag taaggctttg aatagagcaa
     4381 tagaaagagc acagaaaggc attgaaggta aaaattttga acaaagaaag aatgttctta
     4441 agtacgatga tactattaat gagcaaagaa aagtaatata tgatgaaaga aataaggtat
     4501 tggatggttc tgatattgaa gaagagatac aaaaaatggt tatggatatt attacggagg
     4561 ctgctgaaaa gtacttaaaa aaatttagag actatcatgg atattttaag tatttatata
     4621 atacttttat gccagctgat actttattaa ttccagactt agataaaaaa aatgttgaag
     4681 aaatagtaaa tcaaacatat cagatttcaa agcgtgttta tgatcttaag aagatgatga
     4741 ttggaattga agaattggct caattagaga aacgagtatt acttcaagtt gtggatactt
     4801 attgggttga ccatatagat gcaatggatc aactaagaca atatataggt cttaaatctt
     4861 atgcacaaaa agatccattt aaagagtatg ctatagaagg ttatgaaatg tttgaagctt
     4921 taaataggaa tataagaata acaacagtgc aatatctata taaatttaac taaaaaagtg
     4981 acccattggg tcacttttta tatattaaga ttaaagtgaa atttatttta cagtacacac
     5041 tcatatagat aaatatttat aataatgaaa aagaggctga acgtcagcct cttttcctgt
     5101 gtgagaaata cggaaaaaag agatgcttga aatttctttc ttccatttaa tattatacta
     5161 taaagagaat aaaaagtcca tgagttatct ttatgattaa aaaaaaaaat tacaattttg
     5221 ttacttttta gtatacaata ataaatttaa attgactttg ttaattttta ttaataaaat
     5281 atccaaatat tacaatatct acagtttatt gaaataatgt gtaaattata ttataattgg
     5341 taaataagga aaaatggtga aaaccaaatt ttgtaggggg aaatatatga atagaaaaaa
     5401 tctttatgta attatggcag ctgcaatggt aagtacatca gtagctccaa gttttgctgc
     5461 agaagctaca caagtaaaga aacaaacaat aactaaaaag gaagcaacag aattagtttc
     5521 taaagttaga gaactaatgg ctcagagata tacaggaggt tctcaagttg gacaaccaat
     5581 atatgaaata aaagttggtg aaacttcatc acaattaaaa ataataacta atatagacga
     5641 attagagaaa ttagtaaatg ctttaggaga aaataaagag cttcttgtga ctatagcaga
     5701 taaaggacat acaacaaata gtgcaaatga agtggttgct gaagcaattg aaaaatatga
     5761 aaattcagca gacctttcag ctgaagctaa ttcaataaca gaaaaagcta aaactgaagc
     5821 tactgggatt tatggagtgg caactgtaga agcttcatat gacagtacaa aagataagtt
     5881 agtaataact ttaaaagata agactgatgt agtaacttct aaaacgctac aaataggtat
     5941 tggtgatgaa aaggttgatt taacagcaaa tccagttgat atagcaggaa ccaatttaga
     6001 cccttctgca gaaggattta gagtaagtaa aataaataaa ttaggtgtag aaggagctaa
     6061 aaatattgct gatattcaat tagctgaaat aactataaaa aatagtgatt taaatacagt
     6121 ttcacctcaa gatttatatg atggatatag attgactatt cgaggtaata caatagcaaa
     6181 tagtgtagga aaatcaatta gtgatatttc atcaaaagat tcagagacag gaaagtataa
     6241 atttactgtt aaatatactg atacatctgg aaaagcgata gaacttactg tagaaagtac
     6301 taatgataaa gagttaaaag atgttaaaga tgccttaaat ggaaatacaa aagtcaattt
     6361 aatagctggg gaagacaggt atgcaactgc tgtagctata gcaaaagaaa cagaatatac
     6421 tgacaatata gttatagtta attcaaataa attagttgat ggattagcag caacaccact
     6481 agctcaatct aaaaaagctc ctatattgtt ggcgtctgat aatgaaatac caaaagtaac
     6541 tttagattat ataaaggaaa taattaaaaa aagtccatca gctaaaatat atatagtagg
     6601 tggagaatca gcagtatcaa atacagctag aaagcaatta gaatcagtaa ctaagaatgt
     6661 tgaaagattg gctggagatg atagacatac aacttcagta gcagtagcaa aagctatggg
     6721 ttcttttaaa gaagcatttg tagtaggtgc aaaaggggaa gctgatgcta tgagtatagc
     6781 tgctaaagct gctgaactta aggctcctat aatagtaaat ggttggaatg atcttacagc
     6841 agatgctatc aagttgatgg atggaaaaga gattggtata gttggtggtt ctaataatgt
     6901 acctagccaa attgaaaatc aacttgctga tattgataaa gatagaaagg ttcaaagagt
     6961 tgaaggtgaa acaagacacg atactaatgc taaagttata gaaacatatt atggcaaatt
     7021 agataaacta tatatagcaa aagatggata tggaaataat ggtatgttag tagatgcatt
     7081 ggcagcaggt cctctagcag caggaaaagg accaattctt ttagctaaaa atgatataac
     7141 aaactcacaa aagaatactt tagataaaaa actaaatctt ggtgcagaag taactcaaat
     7201 aggtaatggg gttgaattga ctgtagttca aaaaataggc aaaatactag gatggtaata
     7261 atcaataata tagaaaagga ttccttatta tttataggaa tctttttcta tcgacataat
     7321 attcattaaa gtataacatg ctcttgacag ataggaggat atgatagtga tagaagcaac
     7381 attgtttttt tccccacacc aagatgatga aacattaagt atgggtagtg caataattga
     7441 acatgtagaa agaagcgata cacatgttat attatgtact gatggcagta aatcaataat
     7501 aagaaaagta ttagatgatg gagggtcatg ttcttatcat attaaagatg tacacaagta
     7561 ctctttatca gaaagtgatt tttcaaaaaa tagagatgaa gaatttaaaa atagttgtaa
     7621 agcaatggga gttaaagaaa ataatataca tatagaaaat aatagagctc atgatggaga
     7681 attgagtaag gaaaaagcaa gagatataat gctaaaatat ttagaaaaat atcctgatgc
     7741 taaagtgaag accgttacac catataaagc tcttggaata catgaagacc atagagcatt
     7801 aggtgaagct gcattagaat tgtatagaga aggaaaaata aaagatttaa gattttatgt
     7861 agagccatac gattataatg attttaaaaa agttaatcca aatgttagag tatggaaggt
     7921 tttgagtagt caagaagaga aattattaaa tgctatggat gcatacaaga aatggaatcc
     7981 tgaaaatggt aactacgcaa ttggatatca ttctgtaaaa tctcactttg atgaattaaa
     8041 atccaataaa atccaatatg tacatgctcc ataaacatta atataaaaac taggaggata
     8101 tagttaatga aaatatctaa gagaataatg tcgttgttga cagcaacttt ttttatcttt
     8161 gcattatcta tcaatatagc tgatgcttca tcaaaagaga gtctagtggg ctctggtaga
     8221 tgggaaactg caataaaaat tagtcagaat ggatggaaag atgctactaa tgctatacta
     8281 gtaaatgata attctatagc tgatgcatta tctgcaactc catttgctaa gttaaaagat
     8341 gcacctattt tattaactca aagtgataaa ttggatagta gaactaaagc agaattaaaa
     8401 agactaggtg taaaaaatgt atatttaatt ggaggtatga atactctaag caccaatatt
     8461 gaaaaacaat taaaatcaga aaatatgact tttgagagga tttcaggtaa tagtagatat
     8521 gaaacatctt taaaattagc tgaaagatta gataaagaga agagtatttc tgatatagtt
     8581 gtagttaatg gagagaaagg gctagcagat gcagtaagtg tgggttctgc tgcagcacaa
     8641 gaaaatatgc caattatact ttcagatcct aacaatggaa taaaagttgc aaatagcttt
     8701 attagtagta aaaatataaa gaaatcttat ataattggtg gtacatattc tgtatctagt
     8761 tctattgaac agagtttacc taatgtaaaa agattatctg gaaacaatag aaatgaaact
     8821 aatgcaaagg ttctagaaga attttataaa aatacagact taaaaaatgc ttatataact
     8881 aaagatggta tgaaaaaaca gggggatttg atagattctc tagctgtagg tgtattggca
     8941 gctaaaaata gctctccagt tgtattagta ggaaattcac ttgactctag ccaatatgat
     9001 gtgttaaata ctaaaagttt tgataatatt gtgcaagttg gtggaaatgg aaatgaagat
     9061 gctgtaaagg atattcttga tttacaagaa gaaactatat atagagttaa gacagcagac
     9121 gaattggatg ttgctctaag aaaagcagat gctaatgata ttataacatt taaacctgat
     9181 gataataaaa aaattactag ttcatttagt atagaaacaa agaaagctat aactgtagag
     9241 cttgaaggat tgtatacaca aacaataact gtaaatatgc caaatggaga attaactaat
     9301 aagggtacat tagatggaac tgtgaaaata atggatataa aaaatggcac atttatcaat
     9361 gaaggtgata taaatgatat tgacatttat gatccaaatg gatgtaaaat agagaataaa
     9421 agtggtggag atatatgggt tttaactata tctagtagtg ctaaagatgt aaatatagaa
     9481 aataaaggtg atataagtaa ggttacaaat aattctacta gcgttataat aagaaattca
     9541 ggaaatattg atactttaag tggaattaat gcaccagcaa taagtggaaa taaacctaga
     9601 gttgatgata ccgataagga tgaaaaaaga gcaagtggaa tatatcctac ttcagaacct
     9661 ttatcacctg caagagctgg ttatataagt gtaaaaatat tcactaagcc tgaagataca
     9721 aattataaaa tttattatag agtagtaagc agtagaccat ctgcattata tatcggagat
     9781 agaatagata caagtgactg gactgctgta ccatcttctg ttttaccatt tactattaaa
     9841 gcagaaaatg gatattatat agaagctgtt gaaataaatt ctgatactaa gaaagtatct
     9901 agatggggga aatctgacta tatagatgat ggattttatt taaaatcaga aactgcaaaa
     9961 ggattaagtg cagatataga ttttgatgga gattatgtta aattaacagc aacaggtgct
    10021 gattctggat gtacagttta ttatagaata gtatcaaaaa agccagtatc aatgaatgtt
    10081 ggagataaag taactatatc tgattgggat gaaattacta ctaatagagt gaggatagaa
    10141 gcaaacgatg taaaggataa atatatagaa ctagttgaat tggatgattc aactaataaa
    10201 gtaactagat ggggagcaag tgataaggta gtagaatagg tatctaatgt gaaacataaa
    10261 aacagactga ttaataatgg gcagtctgtt tttatttcct aaaaattatc attttactaa
    10321 ataaataatt taaaactata acaattatat tagtaaatac ttttacagta atatcattta
    10381 caaaaaataa atcaatcatt atatacataa taatcatttc tataatacca gaaagtagcc
    10441 taaaactgac aaaaaacaaa aactccttaa acaaaaatat tagttcaatt cttttactct
    10501 caaacacaaa aaacttatta gtaacataag caaataaaac agcaagtatc catgccaaag
    10561 catttgcaac cataaaatta aataaaataa ctctagtaaa aaataaatac gaaactatat
    10621 ttacaagagt agtaaatgct ccaaaaaata aatataatat cgtctctcta tgctttttta
    10681 aaatcaaata taatcctcct aagctaattt aatataaata tacttaaaga tactaacaaa
    10741 cattagtata acacatctat taataaatca atatatctta aaatattata aaaataaaac
    10801 tataatattc tataattaat atgtactcat aatccatatt ttatcaaaat aaaatatgac
    10861 cttaatttag aaatctgata taataaaaat taaatacata taagggggta aacatgagaa
    10921 agtacaaatc aaaaaacttg tcaaagctac tagcattgtt gacagtttgc tttttaatag
    10981 tgtcaacaat acctgtctca gcagaaaacc ataaaactct agatggagta gaaactgcag
    11041 agtattctga aagctatctt caatacctag aagatgtcaa aaatggagac acagcaaaat
    11101 ataatggagt aatacctttt ccacatgaaa tggaaggtac aacacttcga aataagggta
    11161 gaagtagtct tccatcagca tataaatcaa gtgtagcata caacccaatg aatcttggtc
    11221 ttactacacc agcaaaaaat caaggaagtc ttaatacatg ctggtctttt tcaggtatgt
    11281 caacgttaga agcatatctt aaattaaaag gatatggaac gtatgactta tcagaagaac
    11341 atttaagatg gtgggcaact ggtggaaaat atggatggaa cttagatgat atgtcaggga
    11401 gttcaaatgt aacagctata ggatatctga cagcatgggc aggtcctaag ttagaaaagg
    11461 acataccata taatcttaaa tctgaggcac aaggtgcaac taaaccttca aatatggata
    11521 ctgcacctac tcaatttaat gtaacagatg ttgttcgtct taataaagat aaggaaactg
    11581 taaagaatgc tataatgcaa tatggttcag tgacatcagg ttatgcacat tattcaacat
    11641 attttaataa agatgaaaca gcatataact gtacaaataa gagagctcca ttaaatcacg
    11701 ctgtagcgat agtaggatgg gatgataatt attcaaaaga taattttgca tctgatgtaa
    11761 aaccagaatc aaatggagca tggttagtaa aaagtagttg gggagagttt aattctatga
    11821 aaggattctt ctggatttct tatgaagata aaactctact aacagataca gataactatg
    11881 caatgaaatc agtatcaaaa ccagatagtg ataaaaaaat gtaccaactt gaatatgctg
    11941 gtcttagcaa gataatgtca aataaagtaa cagcagcaaa tgtatttgat tttagcagag
    12001 actctgaaaa acttgattct gttatgtttg aaacagactc tgtaggagca aaatatgaag
    12061 tatattatgc accagtggta aatggagttc ctcaaaacaa ttcaatgaca aaacttgcaa
    12121 gtggaacagt atcatattct ggatacataa atgtacctac taattcttac agcttaccaa
    12181 aaggtaaagg agcaatagta gtagttatag acaacacagc aaatcctaat agagaaaaat
    12241 caactttagc atatgaaact aatatagatg catattattt atatgaggct aaagcaaact
    12301 taggtgaaag ctacatactt caaaacaata agtttgagga tataaataca tatagtgaat
    12361 tttctccttg taactttgtt ataaaagcta taacaaaaac atcttctgga caagctactt
    12421 caggagaatc tttaactgga gcagatagat atgaaacagc agttaaagtt agtcaaaaag
    12481 gatggacttc ttcacaaaat gcagtattag taaatggaga tgcaatagtg gatgctttaa
    12541 cagctacacc atttacagca gcaattgact ctccaattct tttgacagga aaagataact
    12601 tagattcaaa aactaaggca gagttacaaa gattaggaac taaaaaagtt tatctaatag
    12661 gtggagaaaa ttctttaagc aagaatgtac aaactcaact tagtaatatg ggtatatcag
    12721 tagaaagaat ttcaggtagt gatagataca agaccagtat atctctagct caaaagttaa
    12781 atagtataaa atctgtttca caagttgcag tggcaaatgg tgtaaatgga cttgcagatg
    12841 caataagtgt tggtgcagca gctgctgata acaatatgcc aataatactt actaatgaaa
    12901 aaagtgagtt gcaaggtgct gatgagtttt taaattcatc taaaataact aagtcttata
    12961 taattggtgg aacagctact ttatcatcaa atttagaaag taagctttca aatccaacaa
    13021 gacttgcagg cagtaataga aatgaaacta atgctaagat aatagataaa ttctacccta
    13081 gctcagattt gaaatatgct tttgttgtta aagatggttc aaagagtcaa ggagacttga
    13141 tagatggact agcagtagga gcattgggtg ctaaaactga ttcaccagta gttctagttg
    13201 gaaataagtt agatgaaagt caaaaaaatg tacttaagtc taagaaaata gaaactccta
    13261 ttagagttgg tggaaatgga aatgaaaatg cttttaatga actaaatact cttttaggaa
    13321 aatagtgaat ggaaagatag taaggatata cattttatta ttaaaaaatg ttcaatagaa
    13381 gtttataaat ataaaataac aaaaaggggt gtctcaaaat gaacttttta gttcatgagg
    13441 cactcttttt tgttgtttta tatttttatc atttaaataa tgaaaaatag gattgtctat
    13501 atttttagat agttttctta ttattgatga aaaatgtaat aattttaaac tgtaaaatag
    13561 tgtaaagtta ttttataata taaatatagt aagattttaa acaaaataag ggggaataat
    13621 aatgaaagca ccaaaaacta ttttaacaat actgacaata gcgcttactt taagtagcat
    13681 ttctataata ccatcatatg cacttacaga ggaaaaatta ataggtaatg gtagatatga
    13741 aactgctgta aagataagtc aaaaggcata cagttctagt cctaatgtag tgctagttaa
    13801 tgacaattca ttagctgatg cactttcagc tacaccattt gcaaaggcta aaggagcacc
    13861 aatacttttg acagaaagtg ataagctaga tgataggact gaaaaagaga taaaacgtct
    13921 aggagctaaa gatatttatt tgataggagg tactgctgta cttaataaag atatagagaa
    13981 caagttaaaa agtaatggac ttaatgtaga aagaattaat ggtaaggata gatatgaaac
    14041 ttcactgatt ttggctaata agcttaagga tataaaagat attaaggaag ttgctgtagt
    14101 aaatggtgaa aaaggattga gtgatgctgt tagcgttggg gcaccagcag ctcaaaataa
    14161 gatgcctata attttgtcta gtcctaagga tggagtagaa gcatttgata aatttataag
    14221 ggatgagaaa gttataaaag catatgtaat tggaggtaca aattctgttt caagagctgt
    14281 agaaaagagt cttcctaatg cagaaagagt gagtgggaaa gatagaaatg agactaatgc
    14341 taaagttata gaaaagtttt acactgatac aaatttaagt aatttatatg taactaagga
    14401 tggtagtaaa aatgagaatc aactaataga ctcgttagct gttggcgtat tggctgcaaa
    14461 aaatgagtct cctgttgttt tggttggtaa taaattaaac acaaaacaaa gagatatact
    14521 tagtactaaa aaattaaata ctataacaca ggtaggtgga aatggaaatg aagaagcttt
    14581 tgatgaaatt aaaagtctac aagaaaaaac tgtgtttgaa gcaaaaactg ttgaggaatt
    14641 aactgatatg ataaatatag caagtcctaa tgatattata aactttaaac caaaagagaa
    14701 tactgtaaat gaagctttta gaatggtaac aaataaacca ataactgtga atataaaggg
    14761 tgattgttca aagactctta cagtagatat gcctaatggt gaagtgaata actatgctac
    14821 tttggtaaat gttatagtta gaaatattgg agaaggtgga tttaataatc atgatacaat
    14881 cactatattg tcagttcgtg acaaaaatgg tagagttata gaaaatacta gaaattcaga
    14941 tatagatacg ttgatgatat tagcttctgc taatgataca aaattgatta atgatggata
    15001 tataggaaaa cttatagata attcatctaa tagtgatata actaataatg gtactataga
    15061 taaaaaagtt aatcaggttg aagatttaga ggctaaagta gattcaattg aaaaagctat
    15121 agattctata agtcaaaaag tcaataaaat acaagatata ctagataagc ttggatttct
    15181 aaagaaattt cttagctaag aatatgtata tttaagtgaa gtgtgtgttg taattgttct
    15241 aaagttatgt agttaaacag gtttatatac aaatagattt atagataaat atgtagttta
    15301 gtatatattt gaataagctt tggggaggta acatggggat tggaattgaa tgtaacaaaa
    15361 aaataaataa aagtattgac aaaactagaa taaagaaaaa taatct
//
LOCUS       DAMJWU010000088        15080 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMJWU010000088 DAMJWU010000000
VERSION     DAMJWU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.092"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(204..347)
                     /gene="scfA"
                     /locus_tag="PC460_002825"
     CDS             complement(204..347)
                     /gene="scfA"
                     /locus_tag="PC460_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF2601702.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(413..775)
                     /locus_tag="PC460_002826"
     CDS             complement(413..775)
                     /locus_tag="PC460_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF2601703.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(833..1126)
                     /gene="yajC"
                     /locus_tag="PC460_002827"
     CDS             complement(833..1126)
                     /gene="yajC"
                     /locus_tag="PC460_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF2601704.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1260..2381)
                     /gene="tgt"
                     /locus_tag="PC460_002828"
     CDS             complement(1260..2381)
                     /gene="tgt"
                     /locus_tag="PC460_002828"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF2601705.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2460..2945)
                     /locus_tag="PC460_002829"
     CDS             complement(2460..2945)
                     /locus_tag="PC460_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601706.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2957..3982)
                     /gene="queA"
                     /locus_tag="PC460_002830"
     CDS             complement(2957..3982)
                     /gene="queA"
                     /locus_tag="PC460_002830"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF2601707.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4300..5319)
                     /gene="ruvB"
                     /locus_tag="PC460_002831"
     CDS             complement(4300..5319)
                     /gene="ruvB"
                     /locus_tag="PC460_002831"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF2601708.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5426..6028)
                     /gene="ruvA"
                     /locus_tag="PC460_002832"
     CDS             complement(5426..6028)
                     /gene="ruvA"
                     /locus_tag="PC460_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF2601709.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6021..6530)
                     /gene="ruvC"
                     /locus_tag="PC460_002833"
     CDS             complement(6021..6530)
                     /gene="ruvC"
                     /locus_tag="PC460_002833"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2601710.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6873..7502)
                     /locus_tag="PC460_002834"
     CDS             complement(6873..7502)
                     /locus_tag="PC460_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601711.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7705..8028
                     /locus_tag="PC460_002835"
     CDS             7705..8028
                     /locus_tag="PC460_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2601712.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8296..9150
                     /locus_tag="PC460_002836"
     CDS             8296..9150
                     /locus_tag="PC460_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601713.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHTIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9336..10772)
                     /locus_tag="PC460_002837"
     CDS             complement(9336..10772)
                     /locus_tag="PC460_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF2601714.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSEKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKILGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10843..12225)
                     /locus_tag="PC460_002838"
     CDS             complement(10843..12225)
                     /locus_tag="PC460_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2601715.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12361..13266)
                     /gene="garR"
                     /locus_tag="PC460_002839"
     CDS             complement(12361..13266)
                     /gene="garR"
                     /locus_tag="PC460_002839"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF2601716.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            13662..14333
                     /locus_tag="PC460_002840"
     CDS             13662..14333
                     /locus_tag="PC460_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2601717.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            14436..14984
                     /locus_tag="PC460_002841"
     CDS             14436..14984
                     /locus_tag="PC460_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601718.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
BASE COUNT         4949 a         2636 c         1746 g         5749 t
ORIGIN      
        1 aaatataaga gtagctactt taaaataaat gctcttacat gattagatat ttttgcataa
       61 ttaaacgata agaaatcaaa tttacttcta cataaataga atatatattt ttatataata
      121 attgaaaata aaagaggata cctcataatg ataaaagaat cactttgaga taccctctta
      181 caaacaacta aatcaataat tttctatctt tcacactctt gatttgcaac tgtacaagaa
      241 gttttacaag ctgattgaca agaagtttga cactctccac atccaccttt tgcagcactt
      301 tgctttaaag tagcttgaga taaagttttt acatgtttat tttccatttt caacgcctcc
      361 atttctaatt gtttcctatt gattatatca taaaactact ttaatataaa ctctatttaa
      421 agttgacacc caatacgcct ccaatgcctc ctgctgctga tattagcaat aacttataaa
      481 tcatccctat ttcaaataca aacttttctt gtgctaaaaa cacagtcaaa aaaatacata
      541 ctatgtacaa aagtcctact aaaagcccat acataagtcc tttttctttc atgtgttttg
      601 aagtgtaaaa ccctcctata gctgcagaaa tagtagttat aaatgatgag accatagtta
      661 tattatctcc accaatatct gtaaatgtca aaaataagtt atacactaat aaaacagcca
      721 aagttattat atatgcatat cctagacctt taaaaatata attagatttt cccataaaaa
      781 aacctcctct caaattaata tatattcgag aaaaggcttt taaattacta atttagtctt
      841 cttctatttc ttctttttta gatttaacag ttcctatagc ccatttttca aatggaactt
      901 tagttctatt tggtcctaat tctaatatta caacatcatc ttcaacttta gctacattag
      961 ctactattcc acctattgta atgaccttgt ctcctacact taggctgctt ctcatttctt
     1021 ttaattgttt gtcttttttc ttttgaggtc taatcattag gaaataaaag attgcgatta
     1081 ctactaccca tagacctata ctcataatta tttgttgctg tggcattaat ttttcactcc
     1141 tttgtaaatt atactctttt gtaaatcata ataaatgttt atataacaat ttactttctc
     1201 tatctttgag tactattgaa tattatatat taaaatgatt ataattaata gtgcaaaacc
     1261 tatatttcat atccatattt tgcaaaaaat tcatttctaa aatcaagtaa tctatcttcc
     1321 attatcgctt gtcttatttg tttcattaaa tttaataaaa agtgaagatt atgagtagtt
     1381 attaatcttg cacccagtat ttcatttgct tttataaggt gtcttatata agctcttgaa
     1441 tagtttttac aagcataaca atcacattca gggtcaagtg gagtaaagtc ttcagcataa
     1501 gttgcatttc taactactac tttaccttgg ctagtcattg cagttccatt tctagcgata
     1561 cgagtaggta gaacacaatc aaacatgtct actcctctta taacaccttc tactaagtca
     1621 tctggactac caactcccat taggtatctt ggtttatcct ttggcataag tggagttgta
     1681 tgctctaaca cgtcatacat aagtggtttt ggttctccaa cactaagtcc accaatagca
     1741 tatcctggta aatcaatatt agttatttct ttcgctgatt gttcccttaa gtctttatac
     1801 atcccacctt gtattatacc aaataaagct tgcttgtcgg tattattatg ggcatcttta
     1861 catcttttta accatcttgt tgttctttct aaagaatttt ttacatattc tctatctgat
     1921 gggtatggtg cacactcatc aaatgccatc attatgtctg aacccaatgc attttgtatt
     1981 tccatagctt tttctggagt tagaaaatgt tttgaaccat ctaagtgtga cctaaattct
     2041 acaccttctt cttttatctt tcttaaaggc cctagactaa atacctggaa ccctccacta
     2101 tctgtaagta ttggtttatc ccaattcata aacttatgaa gaccaccagc tctttttata
     2161 agttcatgac ctggtctcat atataaatgg tatgtgttac taagtattat ttgtgaacca
     2221 atctctttta attcttctgg tgtcatagat tttacagttg cttgagttcc aactggcatg
     2281 aatattggtg tttcaataat cccatgtgga gtgtgtaatc ttcctaacct tgcaccactc
     2341 tgcttacaag tctttatcaa ttcgtatctt actgcgtaca taaagttctc cttaatcata
     2401 tttatttctc attatttctt aagatatatt tgtaataagc tattcatata atttattgtc
     2461 taaatagatt tatcttcgtc ttctttgttc tgttgttcct taattgcttc ttcatcatga
     2521 atactcatta tattaagagc actcttgtct tttgttacta aaaacttttt tatagcaaat
     2581 agtcctgcta tagctataac tcctattaaa acatctatca taccttcatt tttaggcagt
     2641 agcaacattt ttctagcaat tgcgtaaagt aatgcctcaa taaatgctcc tggtatatga
     2701 agagatagca ttactacaag ttcaacacct attactaaaa gtaatatttg tgctaaaaaa
     2761 tcattaagct gactgtattg tactggattt tgaaaatcaa ctatatatgc tccccacata
     2821 agtcttagaa catctatagt tcctaaaaat actgctatta gaactactac tgctaaaaca
     2881 cgttcaaata taaaagcaat ctttaatata gggttattct ttttttgttt ttgtaaacta
     2941 tgcatccatt acctccctac tttataatca tagcatctcc aaaactgaag aatctatatc
     3001 tttcttttac tgcctcatta tatgcattta aaacattatc ctttccacaa atagcactta
     3061 ctaacattat tagtgtagac tctggtaagt gaaaattagt aataagttta tctaaaactt
     3121 taaatttata acctggatat ataaatattt ctgtccatcc actagtttct ttcatctttc
     3181 catactcatt acaagctgat tctatagttc tacaactggt agtaccaaca catattacat
     3241 tttttccatt ttcctttgct ctatttattt tgtcagctgc ttcttggcta accatataat
     3301 attctgaatg catcttgtga tttaaaacat cctctacttt tactggtcta aaagttccta
     3361 agccaacatg tagagttaca aaggctatat ctacaccttt ttcttttatt ttatttaaca
     3421 attcttctgt aaaatgtaat cctgctgtag gtgctgctgc tgaaccatta tgctttgaat
     3481 aaacagtctg gtatctttct ttttcatcta gtctagcagt gatatatgga ggtagtggca
     3541 tatttccaag ttcatccaat atttcttcaa aaattccatc ataatggaat tttataattc
     3601 ttgaaccttc atctgataaa tctacaactt ctccaattag ctttccattt ccaaaagaaa
     3661 atttagtccc tacttttgct ctttttcctg gttttactaa agcctgccaa gtatcttcct
     3721 ctgttctttt taataataaa aactctattt tacctccagt ttcaagcttc tctcctatta
     3781 atctagctgg tataactcta gtattattta atactaagca atctcctgga tttaaatatt
     3841 ctattatatc tctaaatact ttatgttcaa tattacctgt ctctttatct aataccatca
     3901 atcttgagct agctctatct tctataggaa cttgagctat aagttcctgt ggtaagtcaa
     3961 atttaaaatc acttgttttc aatgttaaac ttcctctctt aaaatttctc cagctacaat
     4021 aatatcaaaa aaacaactta ttttccacaa tattaccata atctatcaag aaattgctat
     4081 ttttaacaga caccatatcc acaatatata gatatttttc cacgatatac atgtcttatc
     4141 cacaatatat agatacaata cacacaattt ttatcgtgtt tatccacagt atctacagat
     4201 ataattacca ttttgtggat atgtttgaat ttttagccat tttagaaata ttttaattta
     4261 atgactcttt tattttataa atactcgtat aatctatttt tatttttcat taggatatgg
     4321 aatttttaag tgttcatatg ctaatggcat agctactctt cctctaggac ctctatttat
     4381 aaatcctaat tgaagcaaat atggctcata tacatcttct attgtatttc tatcttctcc
     4441 gatagaagct gccaatgtat ctaaacccac tggtcctcct ctaaattttt ctattatagt
     4501 cataagaagc ttctcatcta caaaatcgag acccaaacta tcgactccta aaagttccag
     4561 agcatctttt gccaccttat cagttatttt tccatcagct ctgacttgtg caaaatctct
     4621 aactcttttt agtaatctat ttgctatcct tggagtccct cttgaacgtt ttgcaagttc
     4681 taatgctcca ttagactgta tctctgcatc taagatgctt gaagacctaa gtactatttt
     4741 tgaaagttca tcaactgtat aatagtccaa tttacaaata acaccaaatc tatctctaag
     4801 tggatttgta agcataccag ctcttgtagt agctccaata agtgtaaatt taggaaggtc
     4861 aagtcttatg cttctagcag atggcccttt ccctattatt atatctaggc aaaaatcctc
     4921 cattgctgga tataaaactt cttcaacact tctattgatt ctatgaatct catctataaa
     4981 taaaacatca ttttcattta aattcgtaag tatggctgcc aaatctccag ctctttctat
     5041 tgcaggtcct gacgtaattc ttaaatttac ccccatctca tttgctatta tacttgctag
     5101 tgtagttttt ccaagacctg gtggtccata taaaagcaca tggtctaact gctcatttct
     5161 agatttagct gcttctataa atatactaag ttgttctttg gatttttctt gacccaaata
     5221 atcttctaaa gtttttggtc ttaaactatt ttctatatct atatcttcca tcttcattgt
     5281 agaagttatt atcctatttt catcttcaaa accttgcatt tatacccctc ctctcatact
     5341 tttgaaaatc tactttatat attttcaaat ttacataaac tccttaaata ttgttttaaa
     5401 atccaaatat tataaaatcc aaatactatt tcaaggattt actcattata tatgtaagtg
     5461 ctttctttat aatagcttct gtattcatac catctttttt acatttttga actgcctctt
     5521 ttgattccga caaagtatag cctaaagcta caagtgcatc tacagactca tcttgtgata
     5581 tcaaggtagg tttttgtgat aataatgttg gttcaaactc tatgttattt ttgtctacct
     5641 tatccttaag ctctaagacg attctttctg ctgtcttttt accaacacca ggagctttgg
     5701 ataattttcc aatgtcttca cttaaaatat atgctcctaa ctgtgctggt gatgcaaatg
     5761 atagtattga aagcgccact ttaggtccta ttttagatac agatgttagt aattcaaaca
     5821 tcttaagttc ttccctactt acaaaaccac aaagactcat atcatcttct cttacaatta
     5881 atttagtata tatttttgtg gcttctccca cttgtaaatt cattattgta ttggatgata
     5941 catttatttt atatcctatc ccattatttt caacaactat actgtctata taaatttctt
     6001 ctactgtacc ttttatataa ctatacatac ttaccaccta tattctttag agttttttca
     6061 agtttgtttg aatgagcatg gcaaatagcc acagctaaag catctgcaac atcatctggt
     6121 tttggaactt tttttagact taaaaatgaa gttaccatct gttgaacttg tgccttatct
     6181 gctctcccat atccaactac accttgtttt acttgaagtg gagtgtattc atatacaggc
     6241 ttcccattat gagcacatgc aagcatagta acacctctag cttgtgcaac tgttatagct
     6301 gtttttacat tcttgttaaa gaataattct tccattccaa cttcatctat attgtaattc
     6361 tttactattg tatcaattcc tttatacaca agttccaatc ttctcgatat attcatatgg
     6421 gcaggtgttg taactgctcc ataatcgatt actttaaact tgctattttt gtattcaatt
     6481 ataccatatc caactatggc tatacctggg tcaatcccca atattatcat tttttaaaat
     6541 tccccctaaa aataaaagaa ataccaaaat gtatttctca taaaaactta tgttctaaat
     6601 cattatatca taaataatat taacaaatca taaaatgctc tatgtaaaat ataaaatcaa
     6661 ataaaaaagt tatatcaaag caagatttta cctcaatata acttttatat ttattacaaa
     6721 taaattattg tatttttata ggaattaaat gtgactacaa ttttaatact aatctattta
     6781 atactaatct atttaatatt aaattattta ttattaattt acttagtatt taacttactt
     6841 agtatttaac ttaaaataat attaatttac tattaacttg caaagtcttc taatcttgaa
     6901 taggcctctt cttcagtatc cataactaca ccttttttga tttcatcttg aactgtttta
     6961 ggaagtgaat ctactcttat ttttgttttt tctactaatg ttttgcttcc actatcatca
     7021 tatttatata gtgctatatt tccattatca ttttcaatag tatatttacc ttttatagta
     7081 acatccattg cttgtacatc tttattatca ctatatttag attctaccaa tatgatttca
     7141 tttttatcca tactctttat ttttttagtt ggatattctt ttttaaaata tgatactatt
     7201 tctgacttcg tcttatctaa taatgactct ggaactattc ctgccattgt aagtgttctc
     7261 ttatagttag tttcattttc gccttcactt tgaagccaaa cttcacaatt tttatttaat
     7321 ccaaaagctt cttttgactt ttcattcata ttcgcactag tacttacaac ttcaccagtt
     7381 tgaactccat tgtgttttaa tcttgtccct atataaactc cgctacttaa tacaaatact
     7441 ccaaccagta ttaaggcaga tattttccaa ggaaataatt cttttttctt cttttcaaac
     7501 attgattacg cctccttttt ctattatttt ctccaagctg taagttttta tacaatttag
     7561 gtaaatttta gaagaaaata tatttatatt tcaaattact ctaagaatag atattttcta
     7621 aaaggtagtt ttctcttaat ggttatattc ttgtaattat atggacataa tttataataa
     7681 ataatcgttt aggaggtaat aactttggaa agaataaact gtaatgtaat taactgttca
     7741 cataacaaca gccatatatg ttatgcagat aaaattttta taaatggtga aaaagctcac
     7801 aatgacagac atacttgttg tagttcattt ttagatgaat ctatttatag taatttatct
     7861 agcaacgtta ataatagaaa ttgtgaggaa ttagtttgta ctgtaaaaaa ttgtaaatac
     7921 aacaaagatg gtagcttatg tacacttgat aatatattgg ttgctcctga aactgataga
     7981 gtaaaccttt atattgaaac atgttgctct agtttcgaat gtaaatagtc atagtataaa
     8041 tataagggtg tcaaatgtaa ttaaactaac atagtgtaga gtatctacaa ctattagttt
     8101 gattatactt gacaccctta tttattttaa ataaaatttc attattataa ccatgtttat
     8161 aatgttctaa ctgtaattaa atttgataaa tttctataaa ttggtataat caatatgtaa
     8221 attgaattta gtaaattaat cataaaaata aaatatcgag tatatagtgt tatactaact
     8281 agaaatgagg tgcttatgag tacattttat attcaatttc tttattttgt aatttatagc
     8341 tttataggat ggtgttgtga aactacatat tgttcagttt tacaaaaaca atttgtaaat
     8401 agaggttttt taaatggtcc attttgccct atatatggat ttggagcatt agcaatagtt
     8461 gctaccttga ctccttttgt acacactatt cctttgttgt ttttgtttag tataattata
     8521 actagcataa tggagtattg tacgagcttt attttagaaa aaatatttaa tatgacttgg
     8581 tgggattatt ctaaacataa atttaatatt cacggaagag tttgtctgga aaactcactt
     8641 atgtttggtg tattatcatt gattgtaatg cttatagtac atcctattgt ggttgacttt
     8701 attaactcta tctctaaaaa tgtgctattt atatttgcta tatctataga aatttatttt
     8761 gttttagatt tagttatcac agtccataca attttacaat taaatggtaa gttaaaacaa
     8821 atcaatctta taattaagga attaaaagat aagaaagaat actacaaact tattacacaa
     8881 gaaactatag aaaacaaact tgaatcttta gttgaaaata gactggatac tttggaagat
     8941 aggtttgaaa tacttaaaga aaaagaaaat tatcgctatg ctaaaaatcg tattgatgag
     9001 ctaaaaaata aacttaatac tttgctgtct aaccacaaat tacttcatag acgtataatc
     9061 aaagcattcc ccaacataag ctctaataag catacagata ttttaaataa aatcaaagaa
     9121 aatattaaat caattaaaaa agataaataa taaaataaac acataagtta aataataaaa
     9181 atactaatac ataagattaa ggtttagaat attttttgta aaatactcta aaccctaatt
     9241 aatatactat tatttttaat cttaatttat aattttagtt tattttaatt ttaactcagt
     9301 aaatttaagc gtttaaactt caatacacta atatactacc ccatcaatat ctcaactgat
     9361 tctctaagtg tgctaatctc tcccacattt ccagggaaaa ttatatatgg catattagga
     9421 aatttgcttt ctttgcctgt catccatact ggtataccag gttttatctg tcccattact
     9481 gtagcttttt ttactcttag tgctttagta ccaacatcac tagaagtaat tccaccctta
     9541 gctataataa aatttggtct tacattaagc ataccaatta tactggtaac tgcatctgat
     9601 atttctacag aaatcataag ctgtttatct ttatcattcg tatccaagtc aaaacgctct
     9661 cgtctagtat aaacagcaac agttttaccc ctgcttattt tttcctctac aacttttata
     9721 actcttttta cctcattttt tagaccattt tcttgaagaa ctaaatgttg attaaattct
     9781 ataaattcta tcggatattt acaattttta agttcttcta actgttgggt agtcttattc
     9841 acatgtgaac caacaagtat aattcctcca ttggtattac cttttgatac taattcatcc
     9901 ttagtaagta gttccttgtc actcactccg ccaagtattt ttgttatggc agcagcgctt
     9961 ctaaacataa attccttacc actatttact gctcgtataa acgcaattgt aaataccttt
    10021 acatctacat aatcggttgc atttacaata attttattga atccttcagc ttttttaagt
    10081 ttttctgtta tagcttcaat atttagctct ctaagttctt ttgacgaaat atatatcata
    10141 tcactagatt tgtactttcc atttgtcttt tcttcacacc actcacctat atcagatgat
    10201 ttatatccaa aagatttatc ttttgcaaat tcagtcattc ctgctggtac aagtatttct
    10261 ccttctttaa cataatgtac attatctata gtaaacctac caccttcttt aaaaaatggc
    10321 attattattt caccctgaaa taacttctcc gataatctct ctacttcgct ttttaataaa
    10381 ttagtttcaa gaggataatg tcctctcata gtagaatcac tacgacttat gattataaaa
    10441 tccttattaa ctttctttga tacatccaca atattctttg aaatttcttt atgtgctttt
    10501 gtagtttgag caacagtaaa tccccttgaa ttggtaagta taaaaaacat actatttttc
    10561 tcattaaaac cttgttccat actatcctta tcccaatcag tataaacaga aatatcatga
    10621 actgtttgta cacctgttgg gtcatcatct aatacaacaa tttttctatc taaatgctta
    10681 agctcatctg caagaagttt atttaatctg tcctcatcta cttgtttaaa agtattaagt
    10741 atatctatgc taattggttt tacttctctc atttaaaacg cccctttatg taatttgtat
    10801 agtctaaatg taatttatat gatataaaca atagtttgtt atttatagac ctggaagtgg
    10861 tataattcca tttataacac taagcaataa aaccattata aatgctacaa ctccagcaat
    10921 ggttcctcca attgtccaag agtttaaagt atctttaact gataatccac taaatcttga
    10981 aagtacccag aatccagaat catttggaag ggagaaacca actcctccag cacatatagc
    11041 aagccctact aaaactggtg acattccagt tgccaaaatt gtaggtccta aaattgatga
    11101 tgttgttact aaggctacag tagcagaacc ttgagaaatt ctaagcaagg cacttaataa
    11161 gaatccaagc actacaactg atatactaag accagacata gtatcaacta agaagttacc
    11221 tataccactt gcatttataa cactgccaaa cgcaccacct gaccctgtta tcaataatat
    11281 tagccctgct gaatcagcag cttcaataac tacatctccc ataggtttat ttatgtattt
    11341 ctttaaaaat cctattgcca ccaatattcc aagaagtatt gctatgtttt tatctcctat
    11401 aaatgcaagt actccactca ttgtagaacc tttttctaag aacatactaa atacatttga
    11461 tataagtata agaagtattg ggaacaataa agaaaacata gaaattccaa aacctggtac
    11521 tttagaagaa tcatcatctt caatttttat gctatctttt ccctgctcta aatttttttc
    11581 tctatcattc tcatcaatag cataagctgg atatttttca aaacgttttc cataaatcca
    11641 tccacctata agagctgctg gaaaagcaac aattatagaa taaagtataa aactacctac
    11701 atttgcattt acatttgctg caactgctaa aggtcctggt gtaggtatta caagtgcatg
    11761 acctactata gtcccaactc ctaaggcaca tacaaaaacc attagagatt tcttagttac
    11821 tctcgaaaca tacttaatta taggcatcat tataacaaaa gctgcatcca taaaaactgg
    11881 aattgaaatt aaaaaacctg ctgtagttac tgctaaagca ctgttttttt ctccagtctt
    11941 agctaacaac ccctttgcaa tagattctgt tgctcttgat tctgaaagaa tatttccaaa
    12001 tattattcca agtccaatta caatacctat actacctaat gtatttccaa atccagtcga
    12061 tattgtggtt gatatttcat ttataggcat tctaaccata aagcctgtaa ttatagatgt
    12121 tatcagtaat gccaaaaacg gatttacttt aaatttaata atcgatacaa gtaaaattcc
    12181 tatagatata acaaatgcta tcaaaagtcc atatcctgta atcataatct ttaccccttt
    12241 atcattctac tttattattt atcaatttta tttttctttt actatgataa cttaacctcg
    12301 ctgtatttat ataatctaat cctaataata tgtatttaat tatattgata tatttaaata
    12361 ttaattattg tgttttttta tttcaacacc tgccaacttt tcaaaatact gtgctattcc
    12421 actatggtca tcttccaaat gtccatgtac ttttaatgtc tgcataattt ctaataattg
    12481 acttgtcata ggtagtggta catctaaatt gtgtgctgtt tgcattacat tcttaatatc
    12541 tttcaaatta atagatattt tcccacctgg tttaaaatca cgattcataa tcattggtat
    12601 cttcgcatcc aatattgtac taccagctag gcctcctctt attgccttat atactttttc
    12661 tgggtctgca cctgcttttg ctgcaagtac aaatgcctca gaaactgctg ctatcgtcaa
    12721 attaacaatt acttggtttg taagttttgt aacacttcca ctaccattat ctccaattaa
    12781 aatagatgag cttcccatta tattaaaaaa tggttctact ttcttataaa tttcttcctt
    12841 accaccagcc ataaaagcaa gtgtaccatc aatcgctttt ggctcccctc cacttactgg
    12901 tgagtctata aattctaagc ccatatcttt taatttattt gcacacaata tggattctgt
    12961 aggagtaact gaactcatat cgactactat acttccttct tttaaagttt ttgccaatcc
    13021 atccatccca aacaatatat cctgcacaat agttccattt ggaaggattg taaatacaat
    13081 atcacattct tgacccattt ctaaaacact tgcgtatttt gctccacatg atataagttc
    13141 atccacagca gatttattaa tgtcatacac caaaagatta catccatctt ttaataaatt
    13201 ttttgccatt ggctttccca ttattcctaa tccaataaat cccaatttca ttttataacc
    13261 ctccatatct tcacattttt gaaaacgttt tttatgtttt agagtatact acataccagt
    13321 ataccggtca atataaaata taaaagtatt tattttaaca ataaatagta atttttaaag
    13381 tcatttatta tatttagtca tcctatataa gttttaatac atactattgt tttcaaatat
    13441 aatatactac ttttacagtt ttgtatttta actaatttca attagtttta attaatctgc
    13501 ctttaatgtt aatcatgctt cacacttcaa taatttttta tattttttaa tcttatctgc
    13561 aatttaaatt gcaaaagatt gaccttattt tcactatgtt gtatgcttaa aattatataa
    13621 gtttttgtat ttttatattg tttagggggg tattattaag tatggctaca tttaaaagat
    13681 tacaagaaca agcatatgac tacttaaaag aattgatact atctggagaa atgatagaaa
    13741 atgaaatata ttctgaaaca aaacttgcta gtgaaatagg tatctctaga acacctataa
    13801 gagatgctct tcaaagactt agtcaagatg gatttataga tataatccca agcaaaggat
    13861 ttagaatcca tcaaattaca gcaaatgaaa ttgtagagat attccaaata cgctctgcaa
    13921 tagaaggatt ttgtactttt ttaattacaa gtcaatacaa agaagctaga gcagtagaaa
    13981 ctataagtaa attaaaacat cttttagata aacaaaaaga tattttatta ggtgataaaa
    14041 atttaaactc atttgcagaa tacgatacat tatttcacac aactatagtt tcatatgctc
    14101 aaaatactga atttgacaaa atgtttaaca attatatgta cagaataaaa aaattagctc
    14161 tcgattcatt gtcacatgaa ggaaggcttg aaacaacatt aaaagagcac acagatattt
    14221 ttaataatat tgcaaatgga tgtattgaag atatttacag aactactctc attcatatgg
    14281 aaactcctaa atacattaat ttagaagatt tttgtaacaa taagttattt tagcattata
    14341 ctttcattta ttaaaaatat atctatctca aattaatttc ctaatttata aaaaatattc
    14401 tataattata ttgtaactaa tacttattct aaggagtgat ttttttgaac gaagatgttg
    14461 taactaatga aactattgac aaagattatg ctattgaaga agttcgtatg gcatgcaaac
    14521 attttggtga tttatatttt tatttttcca aagtgttatt tgaagagttt ggtgaagata
    14581 aaactagcga aatattaaga aaagttttat ttgaaaggtc tgaagaaaga gctattgcta
    14641 tgagagagag agctcatgaa aatggagatg aattaattgc agacaatatt attagcacaa
    14701 ctgatgttcc atttctagga tgggttcctg aatttgaaga gttacattgc ccctatggag
    14761 cttcctggct tttaagattt gaggaaaatc cttggtttaa aaaattcgct tctctttact
    14821 gtgatgttac agatactact gtagctgaag tatttacagg agatacgtcc cataaaataa
    14881 caaaaaacat tctatgggga gataaaagtt gtgaaagaat atatttccat gatgacaagg
    14941 tatctgaagg taaatatact tatggaaata aaaactgtaa ataaataatt taaattttct
    15001 atattcatat actatatttt gtactttttc atataaatat tataactagt cttttatttt
    15061 acttttgttt aacctaaaga
//
LOCUS       DAMJWU010000089        15036 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMJWU010000089 DAMJWU010000000
VERSION     DAMJWU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.093"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            132..1637
                     /gene="ade"
                     /locus_tag="PC460_002842"
     CDS             132..1637
                     /gene="ade"
                     /locus_tag="PC460_002842"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF2601719.1"
                     /translation="MSPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADPHELVNV
                     KGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNNPRILGL
                     GEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDHECITFD
                     EVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDIEKQGHI
                     RWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLDKMEVDS
                     VYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEIVPYQIL
                     TNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAVAPLKGFGIKNGAIATTVAHD
                     SHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFTAEEVQE
                     ITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            1989..2453
                     /gene="bcp"
                     /locus_tag="PC460_002843"
     CDS             1989..2453
                     /gene="bcp"
                     /locus_tag="PC460_002843"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF2601720.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            2463..3206
                     /gene="yaaA"
                     /locus_tag="PC460_002844"
     CDS             2463..3206
                     /gene="yaaA"
                     /locus_tag="PC460_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF2601721.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            3334..5991
                     /locus_tag="PC460_002845"
     CDS             3334..5991
                     /locus_tag="PC460_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2601722.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFAPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      6045..6147
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6257..7522
                     /locus_tag="PC460_002846"
     CDS             6257..7522
                     /locus_tag="PC460_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF2601723.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            7552..8442
                     /gene="metA"
                     /locus_tag="PC460_002847"
     CDS             7552..8442
                     /gene="metA"
                     /locus_tag="PC460_002847"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF2601724.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            8930..9373
                     /locus_tag="PC460_002848"
     CDS             8930..9373
                     /locus_tag="PC460_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2601725.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            9404..11221
                     /gene="ftsH"
                     /locus_tag="PC460_002849"
     CDS             9404..11221
                     /gene="ftsH"
                     /locus_tag="PC460_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2601726.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            11399..14101
                     /locus_tag="PC460_002850"
     CDS             11399..14101
                     /locus_tag="PC460_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF2601727.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            14135..14833
                     /locus_tag="PC460_002851"
     CDS             14135..14833
                     /locus_tag="PC460_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2601728.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         5881 a         1718 c         2757 g         4680 t
ORIGIN      
        1 gtatgtaaga accgtaggaa ctacggggat agcctgtgga gaattagtaa gacatatttt
       61 agtatgcaaa attctgtgaa gcaggaaccc tgtgacttta gtcatgggag gttcagtgta
      121 gcaataaata tatatcacct ggctttattg atgcacatat gcatattgaa tctacaatgg
      181 taatgccaat tgaattatcg aaaaaattat taaaatcagg tacgacaact atcatagccg
      241 acccacatga gttggtcaat gtaaaaggag ttagtgcaat agatttttta ttagaatcga
      301 caaaagatat accacttaat gtatatatta tggtgccttc atccgtacct gcaacaagtt
      361 ttgaaactaa tggagttggt aaatttagtg caaaagacat ggagagttat gtaaataatc
      421 ctagaatttt aggtttagga gaggtaatgt gttttaatga tgttataaat tctgaaaatg
      481 aaatcttgga taaactagag ctttttaaaa ataaagttgt agatggacat gcacctaata
      541 taaatggtaa atcacttcag acatatgtat gtgcaggaat agaaaatgac catgaatgta
      601 taacttttga tgaagtatat gaaaaattga gagcaggatt aaaaatttta ataagagaag
      661 gaagcgcagc aaaaaattta aagtctatag taagtggtat gctaaagcat aacttgccaa
      721 ttgaagaatt tatgttttgc acagatgata agcacttaga tgatatagaa aaacaaggtc
      781 acataagatg gaatataaaa tgtgcgattg atttaggtat ggaacctgtt agggctataa
      841 aagttgcaac ttataattct gcaaaagcat atgggttaag gaaaattgga gcaataggtg
      901 cgggttataa ggcagatatt gttgtattga atgatttaga caaaatggaa gtggatagtg
      961 tatataaaga tggtaatttg gtaaacgaag agatgttttc taattacaat tatgagatta
     1021 aagataaaga attgcttaat acagtcaagt ttaaatatat taataaagaa aaaatacagt
     1081 taaaagtaag tgaaaaaaat tatgtaatgg agattgtacc ttatcaaatt ttgacaaata
     1141 aggtatatga atctttacca tgtgcagatg gatattttgt tccaaataaa gagtattcaa
     1201 aactttgtgt tgttgagaga catagaatga ctggaaatgt agcagttgct ccactaaaag
     1261 gatttggcat aaaaaatgga gcaattgcta ctactgtggc ccatgattct cataatatta
     1321 tagttgcagg ggataatgat gatgatattc tagtagctat aaattactta aaggaaatac
     1381 aaggtggtta tgtaattgta tcaaatggaa aagttcttgc acacctaagt cttcaagtag
     1441 ctggattaat tagtactttt acagctgaag aagtacaaga aattactgat aatatgttgg
     1501 agatagcaag aaaaatggga gttccagagt atgtagaccc ttttattaca ctttctttta
     1561 tggcacttcc agttgttcca caaataagac ttacagattt aggtctattt gatgttgagg
     1621 aatttaaatt tatatagata atataaaagt aataataaat gaggctttct caaaataggg
     1681 ataagtctct ttttattgtt gaaatgataa aaatatattt gatttcatac aaaaaagagc
     1741 atctcatgaa ctaaaaattt tatttttaga tatccttatt ttaattaatc tatcatttaa
     1801 ccttgtttta agcatagata tcttaataaa actattttca gtctatttta acatataaat
     1861 acatgaaaat tatatattta tgaaacttta tacataaaaa ataaaatatt ttatttatat
     1921 atagatttat agggtaataa tataagcata gtataaaatt attaataaaa acaaaaggag
     1981 agatatttat gttgagtata ggaacaaaag caccagaatt tacactagaa gataaagatg
     2041 gaaataaggt aagtatgtca gattttaaag gcaaaaaagt agtagtatat ttttatccta
     2101 aagataatac accaggatgt acacgtcaag cttgtgcttt tagaaatgca tatgatggat
     2161 ttaaaaagga agatatacaa gttattggga taagtaaaga tagtataaaa tcacatcaaa
     2221 aatttgcaga aaagcatgaa cttcctttta ttttactttc tgaccctgac ttagttgcta
     2281 taaaagcttt tgatgtatgg aaagagaaaa aaatgtatgg aaaaactgca ttgggagttg
     2341 tacgtgcgac atatattata gatgaaaatg gaattattga aaaagtcttt gaaaaagcaa
     2401 aaccagacac aaatgcacaa gagattcttg aatatttgga aaaacaggag taataagaat
     2461 ttatgattat aatgttatca ccagctaaaa atatgaagaa tatagaagta tttgatagag
     2521 atttgagttt gccttgcttt atagataaca caaaagaaat agtggaaaat ataaagacat
     2581 ttggtataga agattttaaa aataaaatga aaattaatga aaaattagct gtattaaata
     2641 aaaatagatt tgaaagcatt aaatttgata gattaggaaa ccctgcaatc ttaacttatg
     2701 atggaataca atataaaaat atagaagcag aaaattttac aaggaaagat gaagagtttg
     2761 caaatagctg tattcgaatc ataagtggat tgtatggtgt tgtaaaacca tatgatagta
     2821 tatatgaata tagattagaa atgcagacaa aattacgagt aggtgagttt aagaatttat
     2881 acgaatattg gggaaatcgt atatataaag aactaatcaa agaaaaaaca gctatcgtta
     2941 atctttcatc aaatgaatac agcaaatcca tagaaaaatt tattaaagat agtgatacat
     3001 atattacatg tacttttaaa gtaaataaaa atggcatatt aaaagtggaa tctacacaag
     3061 caaagaaagc tagaggtatg atgacaaaat acattgtaaa aaataggata agagatattg
     3121 aagaactgaa aaagtttaat ttagaaggtt ataagtataa agaaaactta tctaacaata
     3181 gtgaatacat atttgtaaaa gagtagaagt tttgttaaat attaaatggc attgttaaat
     3241 ttaggtaaaa tatacttagt gtgtattgaa ttgaacttga ttttaaaata taattattct
     3301 taaattaaaa aaagaaagga gtggtcttcc aaaatggaaa atcacaaaca gatgccttgg
     3361 cattcaagag caacgcatga aattttagaa agtctagatt catcagagga ggggctcacc
     3421 gataccgagg cggaaaaacg gcttaaagag aatggcttaa atgaacttag aaaaaaacca
     3481 cctagaacaa ctttacaaat gctttggagt caaattacag atgctatggt tatgatacta
     3541 ataggagcag caatattatc tttgatattt ggcgaattta cagaagcttt tgtaatacta
     3601 ataattgtaa cagtaaatgc agttattgga attgttcaag agaaaaaagc agaatcatca
     3661 ttagaagcat taaaaaatat gaactcacct acagctcgtg ttatgcgtga tggtgaagaa
     3721 aatgttattc cagcaagtaa tcttgtagta ggagatattg tttttttaga agatggagct
     3781 atggttccag cagaccttcg tttaatagaa acatcaagtt taaaaattca agaggcttct
     3841 ttaacaggag aatctgttcc atcagaaaaa gatgcagata caatcttacc taaagaatgt
     3901 gtattaggag accgtagcaa tatggcgtat acatcttcaa tagtcacata tggtagaggt
     3961 gtaggtgttg tagtagcaac tggtatggat acagaagtgg gtaacatagc acatctttta
     4021 gattcacaag atgattttga tactcctata aaacgtaaac ttaatactgt tggaaagaca
     4081 ttaagtgtca taggtattat tgtatgcgtt gttatatttg caataggtgc attttatcag
     4141 agacctctct tgccaatgtt tatgattgca atttcccttg ccatatcaat tattccagag
     4201 ggattacctg caacagctac tattgtaatg gctttaggcg tacagcgtat ggctaaaaga
     4261 aatgctttaa ttagaaaatt acctgctgtt gaaacattag gaggagctac agtaatatgt
     4321 tgtgataaaa ctggtacgtt aaccttaaat aaaatgactg ttacacatat tgctgtcaat
     4381 ggagattttg aatctggaaa agtgacatca atagatactg catctgaaaa acatccatta
     4441 gtgtataaag agattgttta tgctggtgca ctttgtaatg atgcatgtat taatccagat
     4501 aaaacagatg aaatattagg tgacccaaca gagggcgcat taatttttat ggccaaaaaa
     4561 tttggaatag atcaagaagc tcttgaagaa gaatatccac gtgtatttga acagcctttt
     4621 gattcagata gaaaatgtat gacaactgtt cataatatag atgaaaagat tattgcttat
     4681 actaaaggag cagtagacga aattcttgac ctatgtacta aaatattaac ttcaaaaggt
     4741 gaaagaaaca ttaccgaagt ggataaaaag aatatacacg aattatgtct taacatgtct
     4801 aaagatgcat tgcgtgtact tggttttgct aagcgtgaga tttcatctat accaaaagaa
     4861 gatagtgaaa atatagaata tgatttaaca tttataggta tggttggtat gattgaccca
     4921 ccaagaactg aggtaattga tgcagtaagt acttgtaaag aagcaggtat aagaaccata
     4981 atgataacag gtgaccataa agttactgcc acaacaatcg ctcatgagtt aggaatatgg
     5041 agtgaagaaa atactgtgat atctggtgat gaacttgata atttgagtga tgatgaacta
     5101 gatgaagctg ttaaaaatac tacagtattt gctcgtgtat ccccatttga taaacttaga
     5161 attatacaat ctctaaaaag aattggagaa gtaccagcaa tgactgggga tggtgtaaat
     5221 gactctcctg ccttaaagtc tgctgatatt ggtatagcca tgggtataag tggtacagat
     5281 gttgcaaaag attcttctga catgatttta atggacgata gttttacaac aattgcttat
     5341 gcaataaaag aaggtagacg tgtatataga aacatacaaa aagttataca atttttgctt
     5401 gttggtaata tagcagagat acttacgttg tttgtagcaa tattattaaa ttgggatact
     5461 ccgcttcttg ctgttcatat tctatgggta aaccttgcaa ctgctacgct tccagccctt
     5521 gctcttggag ttgacccacc aagcagaaat ataatgaagc accagccagt gaaatcaacc
     5581 actttgtttg aaaaaaatct tatacaaaga gttgttaccc aaggtatatt tgtggcaata
     5641 atgactattt tagcatataa aattggtctt aatatagaaa atcattctgt aggtcaaaca
     5701 atggcatttt gtgtattagc attttcacag atgttacgag catttagtca gcgttcaaat
     5761 acagattcaa tgtttaatag aaataatggt aaaaatccat ttcttttaat ctcattttta
     5821 acttctgcat ttttaatagc agtaatacta tttgcacctg tttttagaaa tgcttttaac
     5881 ttaacagttt taaataactt agagtggctt ataacgattg ttttagccat aatgtctgta
     5941 attcaagtag aggttgggaa aattattaaa agaaaaaaat taaaaaaata gttgacaaaa
     6001 ttaggaaaaa tatataagat ataaaacata gaaaaattaa ataaatctta tccagagagg
     6061 tggagggact ggccctatga aacccggcaa ccagtatatt tatatacagt ggtgctaaat
     6121 cctgcggcga aagctgagag atgagtataa ttaagcttat acagagcctg agttatatac
     6181 tcaggcttta tttattttaa ctctcatctg gtaaatagtt taattttttt tactaaaatc
     6241 taagggggat acaagaatgt ataataaaga aacaatatgt gtgcaaggaa attataaacc
     6301 aggtaatgga gaaccaagag tactaccttt atatcaaagt acaactttta aatatagcag
     6361 tatagaccaa cttgctgaat tatttgattt aaaagttgat ggacatatat attcaagaat
     6421 aagcaatcct actattcaag cttttgaaga aaaaataagt ttactagagg gtggagtatc
     6481 ttctgtagct gtatcatcag ggcaatctgc aaatatgttg gcagttttaa atatatgtaa
     6541 atcaggagat agtatacttt gttcttcaaa agtatatgga ggaacattca atttactagg
     6601 acctagtctt aaaaaatttg gtatagattt aatatcgttt gacttagatt caagtgaaga
     6661 tgagatagta gaacttgcaa aggaaaatac taaggttgtg tttgcagaaa cacttgcaaa
     6721 tccaactctt gaagtcatag attttgaaaa aatagcaaat gtagctaaga gaattaatgt
     6781 tccatttatt gttgataatt cattagcatc tccagtgctt tgtaaccctt taaagtatgg
     6841 agcaaatata gttactcatt ctaccacaaa atatttagat gggcatgctt caagtgttgg
     6901 aggaattata gtggatggtg gaaactttaa ctgggataat ggaaaatttc cagaattagt
     6961 tgagccagac ccaacatatc atggtataag ctatactcaa aaatttggaa atgccgcata
     7021 tgcaactaaa gcaagagttc agttgcttag agactatgga aattgtttaa gcccattcaa
     7081 tgcgtatctt actaatttaa atgttgaaac actacatctt agaatggaga gacatagtga
     7141 aaatgcactt aaaatagcta gatttttaga aaaacatgaa aatgtagatt ggattaatta
     7201 cccaggactt gaagataaca agtattatga gaatgccaaa aagtatttat caagaggatg
     7261 tagtggtgtt ttatcatttg gagtaagagg tgggttagaa aatgccaaaa aatttgtgga
     7321 aaaattacag atagcatctt tggttacaca tgtttcagat gtaagaactt gtgttataca
     7381 tccagcttca actactcata gacaattaac agaggaacaa ttaattgcat ctggagtatt
     7441 gccttcacta ataagattat ctgttggaat agaaaatgta gaggatttaa tagctgattt
     7501 aaatcaagct ttaaatttct aatattctaa gagaaagaga agtgaaaaga tatggcttta
     7561 atactaccaa agggattacc tgttattaat aaattattag atgaaggaat agatgtaatt
     7621 tataaagaag attttaaaaa ggaattaaag tatgaagaaa acatagacac taagatagct
     7681 atactaaatc taatgcctat aaagatagat acagaattag atttactaag aaggatagat
     7741 aaaacaggat tcaacgtgtc tgtggaattt attaaaattt caacaagaga aagtaaaaga
     7801 agttgtaatg aatatgttaa agaattttat aaaacatttg atgaagccaa aggagagtat
     7861 tttgatggct ttataataac aggagctcca gttgaacaaa tggagtttga agaagttgat
     7921 tattggaatg aactagaaga aattatggat tattctaaga gaaaaactaa atctacatta
     7981 tatatatgtt gggcagctca agcaagtctt tacaaatact acaatgtaaa aaaacttcca
     8041 ctaagtcaaa aatgttttgg agtatttaag cataaggtag ataaagatag taaaatagta
     8101 gatggatttg aaaatgaatt ttttgctcca cattcaagac atactactgt aaatattgaa
     8161 gctttgaaag agaataaaga gttgagcatt gtgagtcatt ctaaagaagc tggtccctat
     8221 ataattacaa attcaagaga tgtgttcgta atgggtcata gcgagtatga caaatacact
     8281 cttgataaag aatataagag ggatataaat aaaggagata aaatatctat acctcaaaat
     8341 tactatatta atgatgaccc ttcagaagag ccaactgtaa aatggaaaaa acattcagaa
     8401 ttgttgttta gaaattggat aaaaaactat ttgatacaat aaattttaag agtatataaa
     8461 tgttaactaa aaaaatatag aaatttatag gtataaagtt aatcttaatg atactttata
     8521 ccttattttt ttaaaatttg accttataat agtagtttta tattttaaga aaatcacttt
     8581 aggtgtatgt agatagaggt ggctttaatt ttatatatgg tgcgataatg ggagactaaa
     8641 cctttatagt atcaataaat ttgtatttta acttttaaaa gagattaaaa agggagaaat
     8701 cagtgtaaaa tatatataga ttttttatta aattttatta aaaatatcct gtataaaatt
     8761 aaatatcaat aagataacaa aatttcaatt gaagttacaa tattgtaatt gacaataaat
     8821 acttaatact ataatattta atacattaaa cattatagtg ttaagtattt attacagtat
     8881 gcacttcagc ctaaatttga aataaagtat tttcgtaggg agcgtttgta tggatgagat
     8941 gagttttaag aaagagctac tagagttgac tagagatata aatatgaaat ttacaacttt
     9001 attaagtgat ttttatcagc cacttggtat tacagcagtg caagctttga ttttatctga
     9061 gttatgtgaa catggagaga aaaaaataag cgatttaggg aagaacttaa acatgacaaa
     9121 tagtaatgta tcagtaatct gtcaaaggct tgaaaaaaat ggctttttaa atcgcataag
     9181 agatatagaa gaccaacgaa ttgtaaaagt aaaagttaca aataaatcat tggatataca
     9241 agaacatatt tcaagtagta tttttgatag ttattttgaa aatatgacca gtgaaaaatt
     9301 gcaagatatg gaagatataa tagaaggttt agagaaatta aataaacttt taacttacat
     9361 tgattgcaaa taacataaaa ataatttaaa ggagaataaa attatggatg aacataaacc
     9421 ccaaaaaagg tcagcactct attattatac agttataata atagtagtaa tgtttttaaa
     9481 tgctttaata tttccaagtt taatggataa aaaaatggtt gagattgact atggaaagtt
     9541 tctcacaatg ttggatacag gaaaggttaa atctgttgag atagaaaata atagaatagc
     9601 aataaatcca tctagtagca aggataagaa tatatacata actggtagaa tggatgaccc
     9661 agaattggta gatagactaa aaaatgcaaa agtagaattt actaaggtta ttcctaagga
     9721 aaattcacct cttttaagta ttttacttac atggataata cctataggta ttcttatgct
     9781 atttggtaac ttaatgatga agtctatgca aaagcgaatg ggtggtaatg ctatgcaatt
     9841 tggaaaaagc aacgccaaaa tgtatgtatc tgctcaaagt ggaaaaagat ttaatgatgt
     9901 agctggtcaa gatgaggcaa aagaagcttt aactgaaata gtagatttct tacacaatcc
     9961 agaaaagtat aaaaagatag gagcacaaat gcctaaagga gcacttttag taggacctcc
    10021 aggtacagga aaaacactcc ttgcaaaagc tgtagcaggt gaagcaaatg taccattttt
    10081 ctcaatatct ggttctgagt ttgttgagat gtttgtagga atgggagctt ctaggattcg
    10141 tgatttattt aaacaagcaa aagaaaaagc tccatgtata gtgtttatag atgagattga
    10201 tactattggt aaaaagcgtg ataatggaag tggattaggt ggaaatgatg aacgtgaaca
    10261 aactttaaat cagttgttaa gtgagatgga tggttttgat ggtggaattg gagtagtaat
    10321 acttgctgct acaaatagac ctgacagtct tgacaaagca ttacttcgtc caggaagatt
    10381 tgatagacgt ataccagttg aacttccaga ccttggagga cgtgaagcta tattaaaggt
    10441 acatgctcaa aatgtaaatg taagctcaga tatagattat aatgcaattg caaggtctac
    10501 atcaggagca tcaggtgcag aacttgcaaa tattgtaaac gaagcagcac ttttagcagt
    10561 taagtctggc agagatatag tagaacaaca ggattttgat gaatctgttg aaactgtaat
    10621 tgctgggtat caacgtaaag gagctgtaat ttcagataag gaaaagaaaa ttatagcata
    10681 tcatgaaata ggtcatgcac ttgttgctgc tatgcaaaaa cattctgcac ctgtccataa
    10741 gattactata atacctagaa cttcaggagc attgggatat actatgcaag ttgctgaaga
    10801 tgagagtgta cttatgtcta aagaagaagc aattgataaa ataactactt ttacaggtgg
    10861 tcgtgctgct gaagaggtta tctttaacac ttgtacatca ggagcatcaa atgatataga
    10921 acaagctact aaaattgcaa gagctatggt tactcgcctt ggtatgagtg aagagtttga
    10981 tatggtcgct cttgaaactg taagcaatca atatttagga ggagatactt ctcttgcttg
    11041 ttcttccgac actgctgcta aaatagatga agaggtattt aaaatagtaa aggcttgtca
    11101 caaaaatgca attgaaatct tagaaaataa tattgataaa ttgcatgaac ttgctaatta
    11161 tttattagaa aaagaaacta ttacaggtga tgagtttatg gaaatactta acttaaacta
    11221 aaatgattat acacaagata aaaatgccta ttgttaggca tttttatttt acaactaaat
    11281 tttttaaatt catatttaaa tctttacata atcttaatca ttaaaaatac atcttaacta
    11341 caattttata tcggaattga tattataata gtaatgaatg gtgtaaggag gttttttaat
    11401 ggatagacct aatcctgata ttctattaga aaaaattaaa aatgaagaag aaaaactaag
    11461 tagaggtcaa ttaaaaatat tctttggtta tgcagctgga gtaggaaaaa cctactctat
    11521 gttggaatct gcacaaaatt taaaaaaagt gggtgtagat gtagtagttg gatatattga
    11581 accacacact agaccagaaa cattggcttt acttgatggc ttggaaattc ttcctgtaaa
    11641 ggagatagaa tataaatcta taaaactaaa agaatttgat ttagatgctg ctttagcaag
    11701 aaaaccagaa gttatattag tagatgaatt tgcccattct aatgttgctg ggttaaggca
    11761 tacaaaaaga tggcaagata tagaagaatt gctgttagca ggcataaatg tctacactac
    11821 tgtaaatgta caacatttag aaagtctaaa tgacattgtt gagataatta caaacgtatc
    11881 agttaaagag actataccag ataagttttt ggatacaaat acacagttag aacttataga
    11941 tgttgaacct gatgtacttc ttgaaaggtt caatgaaggt aagatatata agaaagaaca
    12001 agctactaga gcaaaaaata atttttttat aaaagataac ctggttgcac ttagagaaat
    12061 cgcacttaga aaaactgcag agagagttaa taaagaagtt caaatgacca gactatcaaa
    12121 gggtgatgta actgtaatac ctacatcaga tacacttctt gcatgtataa gtccatctcc
    12181 atcttcagca aaagttatta gaactgcatc cagaatatca gacagtacat ttgcaaaatg
    12241 gatagcttta tatgttgaaa ctccaaacac agcaagactt ccatttgatg aacaaaaaca
    12301 actacaaaat aacctaaaat tagctaagaa attaggtgga gaaataattg ttttacatgg
    12361 tgaaaatata atagagcaaa tacttagaat tgcaaagctg agaaatgtaa caaaaattgt
    12421 aataggtaga aatcattcaa gtaataaaag atttagtaaa agatttaaaa aagatatagt
    12481 tgacaaatta attgatgaag ttgattatat agatatacat ataattccat ataaatttgc
    12541 ctcagatgtt aaatacaaac ctaaaaagga taaatcatca ataaaatcta aatttaaaat
    12601 tagcaaagtt gattttataa aacttatatt tattactttg atgataagta ttttagcata
    12661 tgcagtacaa agcatgggat ttataagaga aaatatattg ttaatatata tgttgggtgt
    12721 agttttagtt tctatgtgga ctaaagggta tagtacaggt ataataagtt ctgtatttaa
    12781 tatagttctt ttaaattatt ttttcacagc accattatat acattatcta tagcagattc
    12841 aaactatata gtaacactgg ttgtattctc aatagtaggt ataataacta gcactcttac
    12901 gtctaagata cagcatgaag cagaaactgc tgctaaaaga gaagaaaata caaaaatgat
    12961 atatcaaata attaagggat ttttaagatt atcaaataaa gatgatatag taaacaaagg
    13021 tatagaactt ttgtctctta gcttaagtag agatgtaatt tgttatttaa atgtagataa
    13081 gcataataaa tcaaagcttt acaaaaaaaa tactaataat aaggataaaa atgatttgga
    13141 tagtgaagat gaaaaagctg ttgcaagttg ggttttatcc aattcaacag tagcaggaaa
    13201 tgacacggat acacttcctg gttcaaaggg atactatata ccaattgtag gaatgaattt
    13261 aactttagga gtagtgggaa tatcatgtat tgattcaaaa ttggatacag aagatataag
    13321 cttaattgaa acaataattg ctcaaatggc aatagcgctt gatagagaaa tcttgtcaga
    13381 agctaaagaa aataccaatc ttgagattga aagggaaaga ttaagaagta atcttttaag
    13441 agcagtttcg catgacctta gaactccact agcaggaata tctggtgcag taagtacaat
    13501 tataaaaaac aagggtacta taggtcaaga tataatagat gaattactta gtggtgtata
    13561 tgaagatact cagtggttaa taagacttgt tgaaaattta cttagtatga ccaagataga
    13621 tgaagggaaa ttggaagtta aaaaacattc tgaactggtt gaggaaatta tatcagaagc
    13681 gttacaaaaa ataaaaaaaa gaattgaaaa tgcacttata gatattaata taccagaaca
    13741 gatattattt gttcctatgg atgcaaaatt aatagaacaa gttttaatta atttaataga
    13801 taattctttg aaatattcaa aggaagattg taagatagag ataaatgtat atgagaaaga
    13861 tgattatgtt tggtttgaag tatctgataa tggtcctggt atatcaaagg aacttaaaaa
    13921 gcatatcttt gacagatttt ttactggaga agaaggagct aaagattcca gaaagggtgt
    13981 tggacttgga ttatcaattt gtaaatctat aattcaagct cataaaggtg aaattatggt
    14041 agaaaacaat aaagataagg gttcaacttt taagtttagt ttgcctaagg aaaatgaata
    14101 gttttaaatt ttaaaattag ttttggagga agaaatgagt aaaaaactta tattgttagt
    14161 agaggatgat aagacaataa gaaaatttat atctactgca cttttaacac aggattatga
    14221 tgtcaaggag gctataactg gaaaagaagg tatatctata gctgtctcat attctccaga
    14281 tgttgttctt ttagatttag gcttagaaga tatggatgga atagaggtaa taaaagctat
    14341 cagacaattt tcaaatatac caataattgt agtttcagct agagagcaag atagagacaa
    14401 ggtagaagta tttgatgcag gggcagatga ttacttaaca aaaccattct ccatagtaga
    14461 gttattagca agagtaagag tagcatttag acattcacaa gtagaagctc aacaaaaaga
    14521 tgatgttaaa tctacgtttg aagtagataa attattgatt gattttgata aaagaaaagt
    14581 tattgttgat gatgttgaag tccatttgac acctattgaa tataatattc tttcattact
    14641 tgcaaagtat catggaaaag tgcttactca taattttata ataaaagaaa tatggggtag
    14701 tgttataggg aatgagacta agtctttaag ggtctttatg gcaacgttaa gacgaaaaat
    14761 agaaaaacaa cctgcaaacc caagatatat aattacagaa gttggtgtag gatacagatt
    14821 aaatgatgaa tagttgtaat atattaatta ttagattatg tgcttattaa tatatattaa
    14881 aattatatag tttagttaaa ataagaatga agtgtatgtg cttgttgtca gtttagcaca
    14941 tgcattttat tttttagctt ttttagtatc aataattttt tagtatcaat aatatgaaat
    15001 atgttttaaa ataagtatat agtgtaatct gtaata
//
LOCUS       DAMJWU010000090        14840 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMJWU010000090 DAMJWU010000000
VERSION     DAMJWU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.094"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            131..1534
                     /locus_tag="PC460_002852"
     CDS             131..1534
                     /locus_tag="PC460_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601729.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            1561..3105
                     /locus_tag="PC460_002853"
     CDS             1561..3105
                     /locus_tag="PC460_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2601730.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKSGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            3136..4527
                     /locus_tag="PC460_002854"
     CDS             3136..4527
                     /locus_tag="PC460_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF2601731.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            4720..6216
                     /gene="abfD"
                     /locus_tag="PC460_002855"
     CDS             4720..6216
                     /gene="abfD"
                     /locus_tag="PC460_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF2601732.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            6351..6626
                     /locus_tag="PC460_002856"
     CDS             6351..6626
                     /locus_tag="PC460_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601733.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            6650..7957
                     /locus_tag="PC460_002857"
     CDS             6650..7957
                     /locus_tag="PC460_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2601734.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            7977..9095
                     /locus_tag="PC460_002858"
     CDS             7977..9095
                     /locus_tag="PC460_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2601735.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            9382..10302
                     /locus_tag="PC460_002859"
     CDS             9382..10302
                     /locus_tag="PC460_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF2601736.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            10417..11574
                     /locus_tag="PC460_002860"
     CDS             10417..11574
                     /locus_tag="PC460_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2601737.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(11655..12551)
                     /locus_tag="PC460_002861"
     CDS             complement(11655..12551)
                     /locus_tag="PC460_002861"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2601738.1"
                     /translation="MLKKVKYPIGQVKVSDKFKTIKLLSRKYSISSMCKLISVSRSGY
                     YKWLSYSKKSSDRGIKDRIIKDYIIEIHKKYRGTYGRKRICTYLNKILDTPINHKRVY
                     RLMKELGIKSIIRKKVYRRKFKSYEIYDNILNREFRANQPLEKICMDITYIPIGKKFL
                     YMNVAKDLFNGEIVAYEISTKMDTKLVNNTVNQLINMNLAKDCILHTDQGSQYTSRSY
                     SKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPKEMISLINDYIYF
                     YNNERIQLKNGMSPIEYRTHSE"
     gene            complement(12509..12826)
                     /locus_tag="PC460_002862"
     CDS             complement(12509..12826)
                     /locus_tag="PC460_002862"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2601739.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAFDDETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKLSTLLD
                     KSK"
     gene            complement(12963..14462)
                     /locus_tag="PC460_002863"
     CDS             complement(12963..14462)
                     /locus_tag="PC460_002863"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF2601740.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIEDEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         5618 a         1833 c         2681 g         4708 t
ORIGIN      
        1 taaagaaaaa agtgatatta acaagataaa atttttttaa tcagaaatgt taatttaata
       61 acaaaataga gtattgggga gaaaggagaa atatattttt taataatatt tgttaattta
      121 ataacgaaaa atgaaaggga gtgatttagt gaagtttgaa ttgatggatt ttttaatgaa
      181 tccttttgtc cttatgtttg ctgcagtaat tacaggaatt ttatttggaa aaattaaatt
      241 tgggaagttt aattttgggg tatcaggagc attatttaca ggattattta ttggatggct
      301 tgcatatagc ttggggaatc taataatagc aaaaggggaa acagcagcag gatataaggc
      361 agcaactgta atgatgggga atggaattat ttcttctgac ttctttgatt tctttttaat
      421 aatatttgtt gcagcagttg gtctattggc agcaaaagat atgaaagctg tattaaagaa
      481 gtatggagca agatttgtaa ttttaggagt tcttataact tttataggtg gctctatgac
      541 ttatgcaatg actttactat caagtgataa aggaagtagt gcttatgaag tttctggcgt
      601 ttacacagga gcattaacaa gttcgcctgg attagctgct gctttagaaa cagctggtaa
      661 acatgcagaa gatgtatcaa aagaatttga aaaagcttca ataaaagata aaaaagaaat
      721 tttaaaagta gtcgacccag aaggcaaatt ggatgtgaat acaactacat cactgactca
      781 agagcaaata gataagtata ttgcatatgc tgaagctggt gttggtatag gtcatgcagt
      841 cgcatatcct tttggagttt taatagtaat cttaggtgta aatttccttc caaaattatt
      901 taggatggac ctaaaagaag aaagaagaaa atatgagaag gaaatgaagg aagcaagaga
      961 tagtgtttct ggtaaaacga taccagaagt tccatttaat ataataacat ttttcttgac
     1021 atgtttagct ggatacttag ttggaggaat acatgtattt atgggaccac tagggtactt
     1081 cacactaggt gcaacaggag gttcactaat tgtatctcta gttcttggat atataggtaa
     1141 gataggggta gtaaatttca gaatggaaga aaaggtttta aatattttaa aacaaatagg
     1201 gctagtgttt ttcttagcaa tagttggatt aagatatggt ggaaaagtag ttgattcaat
     1261 tatgacatct ggtatgcacc tagccttagt agcaatagca gtaggtgtta cagctatgat
     1321 gataggattc ttagttggca aatacgtatt taaattaaat tggattttat tatctggagc
     1381 tgtctgtggt ggtatgacat caacaccagg tcttggagct gctgttgatg ctcttgatag
     1441 tgatgaccca gcagcaggtt atggtgctac gtatccattt gcattattaa ctaaagttat
     1501 attggttata gttttacaca aacttccaat gtaaataaga ttttatatgg ggggatattt
     1561 atgaataatg aaggagcatt tgaagaatta agtggaagat taagaaataa agaattgtgt
     1621 gatttaataa tgaatgcaga tgaagctgct aaaattatag aggatggcat ggttattgga
     1681 gttagtggat ttacaccttc aggctatcca aaagcagtac cattagcagt ttcagaaaga
     1741 gctaaaagtg gagaaaagat aaaattaacc gtatattcag gagcatcttt aggtccagaa
     1801 gttgatggag catggtcaga agcaggtata attgaaagaa gacttcctta tcaaactaat
     1861 tcaatactta gaaataatat taataaaggt gttgttgatt atatagatat gcacctaagt
     1921 cattctactc aatttttaaa ttatggaact atacctaaag tagatgtagc tatagtagaa
     1981 gcacttgcaa taactgaaga agggaatatt ataccaacta gtgggatagg taattctcca
     2041 tcttttataa aaagtgcaga taaagtaata gtagagataa atcttgcaaa accaatggaa
     2101 atggaaggta tggcagatat atatattacg gaaaatccac caaatagaaa accaatagaa
     2161 ataaatcatc ctaaggatag aattggaaca acatatatac cttgtggttt ggataaaatt
     2221 gcaggagtag taattaccaa tatgcaagat aagacccgtc cattgggggt tgtagatgaa
     2281 gcttcgaaaa aaatatcaaa taatattatt gcttttttaa gagaagaagt aaagtcaggc
     2341 agattatcaa agaatttact tcctcttcaa tctggtgtag gaagtgtagc aaatgcagta
     2401 ttatatggat tatgtgaatc tgaatttgaa aatttaactt gttatacaga ggttgtacag
     2461 gactctatgt tggatttaat aagaatgggt aaagttacaa tggcatctac tacatctgta
     2521 agtccatctc cagaaggatt aataaaattt gaaaaagata ttgatttttt taaggataag
     2581 attatattaa gacctcaaga aattagtaac aatccagaaa tagccagaag aattggtgta
     2641 attgcaatga acactgcaat tgaagttgat atttatggaa atgtaaattc tactcacata
     2701 atggggtcaa agatgatgaa tggaattggt ggttctggtg attttgctag aaatggagca
     2761 ataactatat ttagtactga gtcaattgca aaaaatggtg atatatcatc aatagtacct
     2821 atggtatctc acgtagacca cactgaacat gatgtaatgg taattgtaac agaacaagga
     2881 tatgctgatt taagaggtct tgcacctaga gaaagagcta taaaaataat agaaaattgt
     2941 gcccatccag attataaaga acaacttagg gattatttaa atagagcttg taaaagtgga
     3001 gcaaaacaaa cacctcacat attggacgaa gctttatcat ggcatagtaa atttatgagt
     3061 acaggtacaa tgaaaaaggc tgaaactttt aaaagtgcat tataaaatat ttaataaatt
     3121 ggaggtagat tttaaatgga aaaagcagtg gaaaactttg aagatttaag taaggaatac
     3181 attaatggtt atattgagag agcaagaaaa gctcaaagag agtttgaatg ttatactcaa
     3241 gaacaagtag ataaaatagt aaaaatagtt ggaaaagtag tgtattataa tgctgaatat
     3301 ttagctaaac ttgctgtaga agagactgga atgggagtat atgaagataa ggtagctaaa
     3361 aataaaagca aagcaaaagt tatatataac aatttaaaag ataaaaaatc agttggtata
     3421 attgatatag atagagaaac tggaataaca aaggtagcta aaccagttgg agtagtagcg
     3481 gcaataactc cttgtacaaa tccaatcgtt acacctatga gcaatgcaat gtttgcactt
     3541 aagggaagaa atgcaattat tatcacacct catcataaag caattggatg tagtacaaaa
     3601 actgtagaga tgattaatga agaattagaa aaaataggag ctccagaaaa tttaatacaa
     3661 atacttgacc aacaatcaag agaaaataca agaaatttaa tttcatcagc agatgtagta
     3721 attgccactg gaggaatggg aatggtaaaa gctgcgtact caagtggaaa accagcactt
     3781 ggtgtagggg caggaaatgt tcaatgtatc atagatagag atgtagacat taaagaagca
     3841 gtacctaaaa tcatagcagg aagaattttt gataatggta tcatttgttc aggtgaacag
     3901 tcagtaatag tagcagaaga aatgtttgat aaaataatgg atgaatttaa aaataataaa
     3961 ggttttatag ttagagataa agtacaaaaa gaagctttca gaaatgctat gtttgtaaat
     4021 aaatctatga ataaagatgc agtaggtcaa tcagttcata caattgcaaa aattgcaggt
     4081 gttgagatac cagaagatac aaaaataata gtaatagaag ctgatggacc aggagaagag
     4141 gatattattg caaaagaaaa aatgtgtcca gttatatctg catacaagta taaaagtttt
     4201 gaagaaggtg tagctattgc aaaagcaaat ttaaatgtag aaggaaaagg acatagtgta
     4261 tctatacatt caaatacagt taaaaatata gaatatgcag gagaaaatat agaagtatca
     4321 agatttgtta ttaatcaatg ttgtgctact agtgctggag gaagtttctt taatggtctt
     4381 gcacctacta atactctagg ttgtggttct tggggaaata acagcatctc tgaaaatctt
     4441 gattataagc atctaattaa tatttctaga attgcttatt atatgccaga aaatgaagtc
     4501 cctacagatg aagaactttg gggataaaat ttagacaaaa aatattcatt ctaaattaaa
     4561 gtaggaatat attttaattt agaatgaatt taagacataa attaaattta aattagttta
     4621 ttggactaaa tttaaaatat taagagacat atagaagaag gaagtattat aatttaaaat
     4681 aaataaaaaa atctataaaa gagaaggagt ggttttatta tggcattaat gacaggagct
     4741 caatatattg aaagtttgag aaaattaaat actaaggttt atatgtttgg tgaagaggtt
     4801 aagaattggg ttgaccatcc tatgattaga ccatctataa attgtgtagc agcaacttat
     4861 gacttagctc atgacccaga atatgcagat ttaatgacag ttacttcaaa tatcacaggt
     4921 gaaaaaataa atagatttgg tcatcttcat caaagtgttg atgatttgat aaaaaaagta
     4981 aaaatgcaaa gactatgtgg tcaaaaaaca gcttcttgtt tccaaagatg tgttggtatg
     5041 gatgcgttta atgcagtgta ttcaacaaca tttgagtgtg ataaagccca tggaactaat
     5101 tatcatgata attttgtaaa atacttaact tatatacaag aaaacgattt agtagttgat
     5161 ggagctatga ctgaccctaa gggagataga agtctatcac ctagtgctca acctgaccca
     5221 gatatgttcc ttcatatagt agaaagaaga gaagatggga taattgtaag aggagcaaag
     5281 gcacatcaaa ctggttcaat aaattcacat gagcacctta taatgcctac tataagtatg
     5341 actgaagcag ataaagacta cgctgtatca tttgcagttc cttcagatgc tgaaggagtg
     5401 tttatgattt atggaagaca atcttgtgac actagaaaat tagaagaagg tgcagatgtt
     5461 gatttaggaa ataaggagtt tggaggtcaa gaagctttag tagtatttga taatgtattt
     5521 gttccaaatg atagaatttt cttatgtgga gaatgggatt tctctggtat gttagtagag
     5581 agatttgcag gataccatag acaaagttat ggtggatgta aagtaggagt aggtgatgta
     5641 ataataggag cagcagcttt agcagctgat tataatggag caaacaaagc ttcacatata
     5701 aaagataaac taatagaaat gactcacctt aatgaaagct tatattgctg tggtatagca
     5761 tgttcatcag aaggtcacaa aacagaagct ggaaactatc aaatagattt acttcttgca
     5821 aatgtgtgta aacaaaatgt aactagattt ccttatgaaa tagtaagact tgcagaagat
     5881 atagctggag ggcttatggt tactatgcct tcagaaaaag actttaaatc agaccttaaa
     5941 gttggaacaa gtggaatgac tattggcgaa gtatgtaaca agtattttaa agcttcttca
     6001 gtggcttcaa cagaagaaag aatgagaata ttaagattct tagaaaatat atgccttggt
     6061 tcatctgctg ttggttatag aactgagtca atgcatgggg caggttcacc acaagctcaa
     6121 agaataatga tttcaagaca aggaaacata aatcaaaaga aagaattagc taagaaaatt
     6181 gcgggaataa aaaaagagga agctttaaat ttataaaaag ttgatatata aagggaaatt
     6241 atatattaat gtgtattaat taatgaaaaa atatgtagta taagagatag ttataaaatt
     6301 atggtggttt ttagctaagt aaagaaattt ttaataagga gaatttacac atgacctgtg
     6361 gagttagatt ttgtgggggc tgtaacccta gatacgaaag agggaaggct cttacaataa
     6421 tcaaagagca cttcaatggt agggtagatt ttagtatagc acaagagaac gtcgagtatg
     6481 atttgctact tataattgga ggatgcgcaa ctctatgcgc atcctatcaa caatataaag
     6541 tgaaaaatga atgtgttaag atgtgggatt ttagccatat agaaagagtt ataaatgtga
     6601 ttgaatcagc aatttgtgaa acttaaaaaa gctaaatggg gggatttcat tgagttggca
     6661 agaattgtat caaagtaaat tatgtagtgc tacagaagct gtaaaacaga ttaaaaacgg
     6721 tgatactgta gtatttgcac attgtgtagg tgaacctcct gcactagtag aggctatgat
     6781 tgaaaatgct gaacaatata aagatgttga gattaaacat atggtaagct tgggtagtgg
     6841 tggatatact gctaagggaa tggaagctca ttttagagta aatccaatgt ttgtaagtgg
     6901 aaatgtaaga aaggcaattg aaaatggaga tggtgatttt acacctgcat tcttccatga
     6961 agtaccaaag ttgcttagag aaaaaagatt aaaatgtgat gttgttttag cacaggtaac
     7021 tccaccagat gaacatgggt attgtagctt aggaacatca gttgattata cttatgaagc
     7081 cattaaaact gctagaacag taattgttca agtgaatgac cagtttccta gaacctatgg
     7141 tgaggtagtc catgtatcag agtttgacta tatagtggaa aaatcacaac ctttatttga
     7201 actacaacct gcaaagattg gagaagtaga agaagcaatt ggtaaaaatt gtgcttcatt
     7261 aattgaagat ggttctactt tacagctagg gattggtggg ataccagatg cagttatgtt
     7321 atttttaact gataaaaagg atttagggat tcattcagaa atgatttcag atgggacttt
     7381 ggccctttat gaaaaaggtg ttataaatgg taaatataaa aattttgata aagaaaaaat
     7441 gacagttacc ttcttaatgg gtactaaaaa attatatgac tttgcaaata ataaccctgc
     7501 agtagaggta aaaccagtag actatgtgaa tcatccagca attataatga aacaacataa
     7561 gatggtttct ataaattctg ctatacaagt tgatttaatg ggacaggtag ttgcagaggc
     7621 tatgggactt agacaattta gtggtgttgg aggtcaagtt gactttatac gtggagtgtc
     7681 tatgggtgaa gatggaaagg caattatagc tatgccttca ataactacga aaaaagatgg
     7741 tacagtaatt tcaaaaatcg tgtctattgt ggatgaaggt gcacctataa cgacatcaag
     7801 aaatgatgtt gactatattg taacagaata cggtatagca gaattaaaag gaaaatctct
     7861 tagagaaaga gcgagaaatt taataaatat agctcatcca agtgtaagag aaagtctagc
     7921 agtagaattt gaaaagagat ttaaggagaa atattaataa tagaggggtg ttaataatgg
     7981 aagcacttag agtagttcca aagatatttt actttgatac atttaaagaa tttaatgaag
     8041 aatttaaaat aggaaaaaat gatttagtaa ttactaatga gtttatatat gagccatata
     8101 tgaaacctct tggaatagat acaaatttaa tttttcaaga aaagtttgga acaggtgaac
     8161 catctgatga aatgatagac tccatgacaa aggaaatgaa gaaatacaat tttgatagaa
     8221 ttattgcatt tggaggagga acaatagttg atatatgtaa gatattagca cttgatgttc
     8281 cagaaaagtc tattgattta tttgaaggag aagtatcacc taaaaaagta aaagagttag
     8341 tagtagttcc tacaacatgt ggtacaggta gtgaagtaac aaatgtagct attgcagagt
     8401 taaaatctaa acatactaaa aaggggcttg cagtagaaga aacatatgca gactatgcag
     8461 tgttaattcc agagacagta aaagggttac cttacaaatt ctttgttaca agttcggtag
     8521 atgctcttat acatgctatt gagtcttatc tttctccaaa agcaagtcca tttacagaga
     8581 tgtattctct acaagcaata aaaatgatta tggatgggta taagaagata gttgataaag
     8641 gagaagaaga gagatttaat catcttagag actttgtact tgcatcaaat tatgcaggaa
     8701 ttgcttttgg aaatgctggt tgtgcagcag ttcatgcact atcttattca ataggtggag
     8761 ctttccatgt tgcacatggt gaagcaaatt atcagttctt tactgaagta tttaagatgt
     8821 attctagaaa aaaacctaat ggaaaaatta taaaatgtac aaaaatttta gcagatgccc
     8881 ttgaatgtga ccctaactgt gatgtttatg gagaattaga aaagttttta aataaattaa
     8941 tagctaaaaa agctttgaga gaatatggaa tggtggaaag tcagatagat gaatttacag
     9001 atagtacaat agcaaatcaa caacgactac ttgctaataa ttatgtagag cttagtagag
     9061 aggaaataag agagatattt gcaaatcttt attaatacaa ttttttattt atatgtatag
     9121 tcaacataaa caatgctaat tatatttatg tacaaataaa aataagggaa cttaaatttt
     9181 aattaaagtt aagttctctt atttttatat ggagttaaat ataagtatat ttttaattta
     9241 aaatatgctt atatttaagt ctattacttt atttgaaaca atttgtagaa tttcaaattc
     9301 cttgacagta ataagtaagt aattgtataa tcaaactgca atatattaat aatattagtg
     9361 aaaatgaaag gattataaaa tatgtataaa tcagaaaatt tttcaaaaaa atctgtatta
     9421 ataaattcat ttttaagtaa atttttaaaa ataattggat ggggtttggt aagcttattt
     9481 acattatcag ccttagtact ttcaactatt tccattataa ttggatgggc tgtaatactt
     9541 cctattcaag tatgtgcagt atttttactt ataggaggag tttgtatttt taaaggtaag
     9601 agattaggag accaaatttc tcgttataaa aaatattgta caattataaa caatagaaat
     9661 attattccag tagaacttat tgcagaaaac acatctaaat cattaaattt tataataaaa
     9721 gatgtacaaa agatgataga caaaaactac tttataaata cttatattga taaaagaaat
     9781 aatcagattg tgttaacaaa tgaagatttt gttccaccaa gatatgaaga tgtagcatat
     9841 gaagtcaaag aagaagaaaa gactgatgaa tccaatgaag ttgatacaat tataaataaa
     9901 ggaatgaatt atctaacaca aataaaagaa gctaataaaa atattaaatc agaatcaatg
     9961 ggcgataaaa ttgtacaagt agaagatgtt acaagtaaaa tatttgatgt tgtaaaacat
    10021 gacccatcaa aacttactca aatacaaaaa tttatggatt attatcttcc aaccacttta
    10081 aaactattaa attcatatca tacattggaa gaacaaggaa ttgatagaga aaatataaca
    10141 acaactatgg aaagtattga aaatacaatg aatacgattg tagtagcttt tgaaaatcaa
    10201 ttggactatc tgtttgaaga tgaagccatt gatatttcta cagatattac tgttttagag
    10261 aatatgttag tccaagaagg attaacgagt ggtaaatttt aacttcaata gaaaaaatat
    10321 tctgttattt ttaaacaatt aattagtcat aaagtataaa atgtgtatta tataagttaa
    10381 tcagtacttt atgaaggaag aggagagaaa gatacaatga ataatttaga tgatatacca
    10441 gttatgccaa ctttgacact tgacccattt ggagaagaat caaacactaa tgatatagat
    10501 aattctgact tattaatgaa aaaggatgaa aaagacccag aagaagaaaa actgtctgaa
    10561 tctgaacgca agatggtaaa ggaatttgcg gaaaaaatag atattacaaa tacaaatatg
    10621 attttgcaat atggagctgg tgctcaaaaa aaggtagcaa gtttttctga aacagcttta
    10681 aagagtgtaa agacaagaga tttaggagaa gttggagata tgcttacaaa tctagttact
    10741 gatttaaaag cattttcagc agatgaaaaa gaacaaagtg gttttttagg aatttttaaa
    10801 aaggcaaata ataaaatttc aaatttaaaa actaagtatg atagtgcaga ggtaaatgtg
    10861 gataaagtaa gcaaagagct tcaaaagcat caggttaaaa tattgaaaga tatagcaatg
    10921 cttgataaga tgtatgaatt gaacttagct tactcaaaag agcttactat gtatattata
    10981 gcaggaaaac aaaaattaaa ggatatgaaa gaatatgaga tgccaaagtt gagagaaaaa
    11041 gctcgtttat ctggttcaac agaagatgct caatctgtaa atgatatggt ttcactttgt
    11101 gacagatttg aaaagaagat tcatgactta gaattaacta gaatggtatc gcttcaaatg
    11161 gctcctcaga taagattggt tcaaaatagt aataatctga tggcagaaaa gattcaatca
    11221 actatagtaa ataccattcc attatggaaa aatcaaatag tgcttgctct tggaatttct
    11281 cacttaaatc aagctatgaa agcacagcgt gaagttagta atatgacaaa tgagctttta
    11341 ttaaagaatg cagaaacttt gaagatggga acaatagaaa cagcaaaaga atcagaaaga
    11401 ggaattgtag atatagacac aattaagaaa actaatcaat ctctaatatc tactattgat
    11461 gaggtagtta agatacaaca tgatggtaga ataaagagac aagaagcaga agttgaatta
    11521 tcaaaaatag aaaatgaact taaaagcaag cttcttgaat ttacagttaa ataaaaatta
    11581 tactaagata aaaagagact gtctcaaaat tggatatgtc cctgtcaagt agacagactt
    11641 aaaaaaggct atctttactc ggaatgagtt cgatattcaa tcggactcat tccgtttttt
    11701 aattgtattc tttcattatt ataaaaataa atataatcat ttattagact aatcatttcc
    11761 tttggatctg tagtatcaat taaatatatt aattcagatt taaaatgact gaaaaaactt
    11821 tctataggag cattatccca gcagttgccc cttcgggaca ttgattgtat aataccatta
    11881 tccttaagcc ttttagagta agatctactt gtatattgtg aaccttgatc tgtatgtaaa
    11941 atacaatctt tagctaaatt catatttatt agttgattta ctgtattatt aactaattta
    12001 gtatccattt ttgtacttat ctcatatgcc actatttcac cattaaataa atctttggct
    12061 acattcatat ataagaattt tttaccgata ggtatgtatg ttatatccat acaaattttt
    12121 tctaatggtt gattagctct aaattcacga tttaatatat tatcatatat ctcatatgat
    12181 ttaaacttac gtctatatac tttctttcta ataatagatt taatacctag ttctttcatc
    12241 aacctatata ctctcttatg attaataggg gtatcaagta ttttatttaa ataagtacaa
    12301 attctttttc taccataagt tcctctatat tttttatgta tttcaattat ataatcctta
    12361 ataattctat ctttgatacc cctatcggaa gattttttag aataacttag ccatttatag
    12421 taaccgcttc ttgacacact tatcaactta cacatactag aaatactata cttcctagac
    12481 agaagtttaa ttgttttaaa tttatcgctt actttgactt gtccaatagg gtacttaact
    12541 tttttaagaa ttcattctcc atgcgtaagt acttcaattc ttcttcaaca gatttaaatt
    12601 ttctattatc attttctaga ctaatatttt taaatctgcc cctagtttca tcatcaaatg
    12661 cagttttgcc tttattttta taatctttta cccagttttg aacttgtgtt ttgcttctta
    12721 tattcagcat ctcagcaatt ttgtatgatg agtatccttc ttttatatat aagtttacag
    12781 cttttagctt aagttctttg ctatattttt tgtgtttttt agacatagaa aaaccccctt
    12841 agagtatacg ctttttagac ctattataat agccttttat ttaaatgtct actataaggg
    12901 gattatacta aaatagagat aagtctcttt ttcattgttt aaatgattaa agtatattta
    12961 tattataaat ttctattatc ttttaacata acatcatgag ctccaccttc aactatacta
    13021 gtagaagata caagtgtaag tttagcattt ttttgaagtt caggaatgct aagtgctcca
    13081 cagttacaca tagtagaacg aactttactt aaagaaagag ttacattgtc tttcaaagaa
    13141 ccagcataag gaacataaga gtcaacacct tcttcaaaag aaagtttctt atctccacca
    13201 aggtcatatc tttgccagtt tctagcacga gcagaacctt ctccccaata ctctttcata
    13261 taactaccat ttatattaac tttgttagtt ggactctcat caaaacgaga gaagtatcta
    13321 ccaagcatta taaaatctgc acccatagct aaagctaaag ttatatgatg gtcataaacg
    13381 ataccaccat cagaacaaat tggaatataa atacctgttt tttcaaagta ttcgtcccta
    13441 gcttgagcaa cttcaattat agaagtagct tgtccacgtc ctataccttt ttgttcacga
    13501 gttatacata tagaaccacc accaatacca actttaacga agtcagcacc agcttcagct
    13561 aggtaaagga atccctcacg gtcaactaca tttccagctc ctaccttaac tgtatcacca
    13621 tatttttcac gaatgaaatc aagtgttatt ttttgccact cagaaaaacc ttctgaagaa
    13681 tctatacata aaacatcagc accagcttca actaaagcag gaacacgttc agcaaagtct
    13741 ctggtattaa taccagctcc aactacatat ctcttagaag aatctaaaag ttcaagtgga
    13801 ttttctttat gagaagagta atctttacgg aataccatat acataagatt accattgtca
    13861 tcaagtacag gaagagaatt aagtttgtgg tcccatataa tgttgtttgc ttcctttaaa
    13921 gtaacatctt tgttagcata aactatagat gacataggag tcataaattc actaacttta
    13981 gtatctaaat ccatacgact tatacgataa tcacgacttg caacaatacc taataattta
    14041 ccagttgaag ttccatcttc agtaacagct actgtagaat gtccagtttt ttcttttaag
    14101 tcaagtatgt cttttaatgt gttatcaggt ttaatatttg aatcacttac aacaaaacca
    14161 gctttgtggg atttaacacg tgctaccata gcagcttcat cttcaatagt ttgagaaccg
    14221 tatataaatg aaataccacc ttctttagca agtgcaatag ccattttgtc atcagaaaca
    14281 gattgcataa tagcagaagt caaaggtata ttcatataaa tatctgcttc ctcgcccttt
    14341 ttaaatttag taacaggtgt ttttaaactt acattagcag gtatacaatc agtagaagag
    14401 aaacctggta caagaaggta ttcactgaaa gtatgtgatg gtgtatcaaa ataaaacgcc
    14461 attttttaat cactccttat atttttatac tttttataat tgatagtatt aattataact
    14521 tattctttaa ttttattaaa gtattttgtc aaatattgag atttttgaaa aatttaaact
    14581 attttaattg aatttgttta atatatattg ccttataggg taaagttgta tattaaataa
    14641 tgtttagtgt ttaatagtag ctaatttgaa attaatcttt agttattcat ataattacat
    14701 ttgtatagtt tatattgata atattgctag agagtgcata aacctcaaaa atacagataa
    14761 attaaatcta tattagtata tccaaaagta catttaataa atttatttaa ctgaattatt
    14821 attttcattt ctattaaatt
//
LOCUS       DAMJWU010000091        14581 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMJWU010000091 DAMJWU010000000
VERSION     DAMJWU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.095"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            131..271
                     /locus_tag="PC460_002864"
     CDS             131..271
                     /locus_tag="PC460_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601741.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(446..766)
                     /locus_tag="PC460_002865"
     CDS             complement(446..766)
                     /locus_tag="PC460_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2601742.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(886..1623)
                     /locus_tag="PC460_002866"
     CDS             complement(886..1623)
                     /locus_tag="PC460_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF2601743.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1838..3661
                     /gene="acd"
                     /locus_tag="PC460_002867"
     CDS             1838..3661
                     /gene="acd"
                     /locus_tag="PC460_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF2601744.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3876..8174
                     /locus_tag="PC460_002868"
     CDS             3876..8174
                     /locus_tag="PC460_002868"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF2601745.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8378..8839
                     /locus_tag="PC460_002869"
     CDS             8378..8839
                     /locus_tag="PC460_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF2601746.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8869..10023
                     /gene="nusA"
                     /locus_tag="PC460_002870"
     CDS             8869..10023
                     /gene="nusA"
                     /locus_tag="PC460_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF2601747.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10030..10311
                     /locus_tag="PC460_002871"
     CDS             10030..10311
                     /locus_tag="PC460_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF2601748.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10301..10612
                     /locus_tag="PC460_002872"
     CDS             10301..10612
                     /locus_tag="PC460_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF2601749.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10634..12574
                     /gene="infB"
                     /locus_tag="PC460_002873"
     CDS             10634..12574
                     /gene="infB"
                     /locus_tag="PC460_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF2601750.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12617..12997
                     /gene="rbfA"
                     /locus_tag="PC460_002874"
     CDS             12617..12997
                     /gene="rbfA"
                     /locus_tag="PC460_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF2601751.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12978..13655
                     /locus_tag="PC460_002875"
                     /pseudo
     CDS             12978..13655
                     /locus_tag="PC460_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
     gene            14295..>14581
                     /locus_tag="PC460_002876"
     CDS             14295..>14581
                     /locus_tag="PC460_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601752.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KSDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKE"
BASE COUNT         5844 a         1667 c         2720 g         4350 t
ORIGIN      
        1 tagatatatc ctaattatgt caaaaaataa aaaaattttt atttattaga gtttttggaa
       61 ataataataa cagatattat aattaattaa cagtttttgt aagtatttga caatagagag
      121 ggtgtaacaa atgggaataa tagagtcagc atcaaaattg gctgagatgg ttcatttatt
      181 ggctgttgaa aaggggataa ctgatataga agcatgggat gaagctgtga aagaatacag
      241 caaaatttat gaagaaagaa gaaatgaata gaatgataaa aaatgaaaac ttgatgataa
      301 aaaataactt tatttaaaga aattcaattt ttatagaagt attaaaaata atataaataa
      361 aaagatggta tttcaaatgt aagtaaaaaa tcattttggg ataccatcct tattatttat
      421 tttaataata tataagtaat aaatcttact tatccatatt ttttatgtaa cctctttttt
      481 ctaacttttt tatatgctcc ttacgtattt catcaagatt aataccatat tgttcttcta
      541 gcttaaacat catagtaaca catgtctgcg ctacatccat tagttcctta gcagttttct
      601 ttataatatc aacttcatct aaatcattat tttctccact tagatttctg aatttaccta
      661 tacattcagc taattcacct tgttcttcac ccattttgat aaatgtagat tctagtgttg
      721 ggtctaaatt attaagctct ggtaaagata tagtttttag tttcataaaa gctcctttca
      781 agttattcgt atttatacta aaacatcata acaaaaataa tactaaaaaa aaactttaaa
      841 ttcaacataa aaacttacat atataataaa aatcatgaaa taaatctata tttgtcttaa
      901 tacagcctca tataaaactt ctccaatagg tttatttatt acaagagaag ctgacttatc
      961 atagactgta tttgctttat taataagagc aatataatca cctttgtaat aatctataaa
     1021 acttgctgct gggtacacag ctaatgatgt acctcctata atcaataaat cagcattact
     1081 tatagcagaa attgttttag taatgacatc actgtcaaga gcttcctcat aaagtactac
     1141 atctggtttt actatagaac cacagttgtc acagtaagga atattacctc ccagatttaa
     1201 catagattct aagtcaaaaa acttaccaca tttagtacaa taatttctat gtacagaacc
     1261 atgaagttct agcacatttt tacttccagc catttgatga agtccatcaa tattttgagt
     1321 tatgacagct ttaagcttac ccatttcttc gagttttgct aaagctatat gagcattatt
     1381 gggttttgca ttaggatata ttaatttatc cttataaaat tcaaaaaatt cttctggata
     1441 tctaacaaaa aaagtatgtg aaactaattg ttcagcagta aattgtttat tgagtttttg
     1501 gctaaataat ccagtagaac ttctaaagtc tggaatatta gattcagttg aaacacctgc
     1561 tccaccaaaa aatacaatat tattgtgatt agctattaag tcttttaagc tatttgcatc
     1621 catagttaca cctccaaatt attatatata aattgtacta catattaggt cgtttatgtt
     1681 ttattaattt aaataaatat atagtttaat aagttaaata aagggaatat aaataaaatg
     1741 tatacagagt ataaatagac aatatttact aaaataacaa gttgtcgtat aattatatta
     1801 aaattgtttt agtgtataag aagggcgggg gaagtacgtg aagaaaaaag cagctttagc
     1861 aacattggct atgttaccct taggtgtggt aaatgcacat gctgatggag atataggtat
     1921 agtgactata aattatttaa atgtaagaaa tgaaccaact gccgaaagta gcatagcctt
     1981 tgttgctaaa aaagatgata aagttttgat taaagactct tctaatggat ggtataaaat
     2041 aaaagctgaa tctggacaag aaggttgggc ttcatcaaaa tatatagcaa aatcaaatag
     2101 tgactcttta agaacgtcta caaataaaga aaaacaagtt atttcaaata gtttaaatat
     2161 gagaaatgga gcaggtacaa gttacagagt aataactgta ctaaaaaaag gtcaaaaagt
     2221 agaagtaata tcagagagta atggatggtc taaaatcaaa tatgatggaa gactaggata
     2281 tgtatctagc tcttatttag gagacgtttc aaactcaact aataaatcaa aaactaaaca
     2341 agtgaataca acttcactaa atgtaaggag tggaccaaat acaagttatg gtttgttagg
     2401 caagttgcca aaaggaagta aagtagaagt aatatcagaa agtaatggat ggtccaaaat
     2461 aaagtataat ggaaaagatg cctatgtatc tagtatgtac ttatcggatg taagtcaaag
     2521 taattcagac aattctagtc aaagcaatga taaaaagaat actgataagg ttgtaaatac
     2581 agcttcttta aatgtaagaa gtggaccagg ttctacatat agtaagttgg gaaaagttta
     2641 taaaggaagt aaagtaactg tactatcaga aagtagtgga tgggctaaga ttaattttaa
     2701 caataaagaa gcatttgtag taggcaatta tttatctacc tcagcagata cttcaaataa
     2761 taactcaaat agtaactctg ataacagttc taatagtaat ggtaataatt cttcatcatc
     2821 aggccaagta aatggtatgt caggtataag tggggctaaa attgattata aatctttgag
     2881 ttatacttta gaatctcata taagtaaaca agttgagaaa gcagcatcag gaggaaatgt
     2941 gatagctcca agtaatagga aaagtactcc aagtcctgag tttagtactt tttcagctca
     3001 aagaacaagc tcatttgtaa atgcaagttc aagtgatata gaatattatt taaatcctaa
     3061 gaattttaca aatactacta aaggtatgat gcagttttta aagattaaca gttatagaga
     3121 tggtatttca gaatcaagtc ttaactcgta tcttaatggt ttatcttcta gtgtatttaa
     3181 aaaccaggga gcagccttta taaatgctgc caagaagtat aatatagatg ttgtatatct
     3241 agtatctcat gctatgtggg aaactgctta tggtaagtct acacttgccc aaggtcaaac
     3301 attaacatct tataagggac aacctcttag taagccagtt aaagtataca atttttttgg
     3361 tataggagca atagataaaa gtgctaatgt ttcaggtgca gaagcagcgt attccaatgg
     3421 ctggactagt gtagaagcta caatagatgg ctctgcaaag tggatatcac aaaattacgt
     3481 aaatagttct aagtacaatc agaatactat ctacaagatg aagtggaatt atgattatac
     3541 atggcatcag tatgcaactg atgtaaactg ggctaatgga atttctggaa ttatggaaaa
     3601 tttaattggt ctttatggag gaggaagtag tttggttttt gaagttcctc aatataagta
     3661 atacataaat aaaaaatata gactatgggt agttttcatt ttatttaaat tatctatagt
     3721 ctttttgtat ataatttata atctgtaaat ttaatgttaa caaaactttt atgttataat
     3781 attaattcta agtaaatata agtgagtaat aggtagaaat agataaaaag tggtaataat
     3841 atatataaag ataattttga gaaaggagga tatatatgga aagtattaag gaatatttgg
     3901 ataagttaga aattaataat agtggtcttg gaaagcaact taaagaagtt tatataaata
     3961 gagtagttta ttttaaggaa gataagatag tttattttta tctaacttct aaagatattg
     4021 tttctcatga acttttagat aaatttaaag aagaattgat gtataaactc gattatttta
     4081 aagatatgag gatgaagata agatttactg gtcttgaaag aaaatccaat aaagatgtta
     4141 taaaaaaata ttggaataat atattgtata tattaaagta tctttgtcca tctattgcag
     4201 ggtggtataa acaagtagaa tttctttgtt tagaagaaga attaaaaata aaacttccaa
     4261 aaggtatatt ttatgagcga ttaatgaaaa aaaatgtaac ttatgtactt aaaaccgtgc
     4321 ttagcgaaga gttgggatta gatttaaata taacaataga aaaagctgtt gatgaaaagg
     4381 taaataaaga aagactcata agaatcaatg atagagaaat ggaagaaaaa attagggctt
     4441 tagaaattgg aaaagttaat aactgtgaag aaaatgaaga ggaaagctat gtcattaagt
     4501 ctgatgtaga tgaaaattta atctatggag ataatgcaaa tgctatgatt gaaaatatag
     4561 ttgaattaaa tgcatcttct ggaacagttg cagttgttgg agatatattt gatgttgaca
     4621 ctaaagaact taaaaacgga aaaatactta tgatagcttc aattacagat tatactagct
     4681 caataagctg taaacttttt ttaacagata caaataagga tggagtactt gaaaatgtta
     4741 aaaaaggagc atacttaaaa atcaagggtg acatagttta tgatacatat caaagagaaa
     4801 ttagtatgat gattagtggg ataagaaaag aaactagaat agagagagaa gataaatcag
     4861 aggaaaaaag agtagaactc catgcacata cacaaatgtc ttctatggat gctatttgtt
     4921 ctgttaaaaa gcttgtagag cgtgcagcca aatggggtca ttctgctatt gctataacag
     4981 accatggtgt tgtacaagga tttccagatg ctatgaatgc aggtaagtca aataatataa
     5041 aaatacttta tggagtagaa gggtacctgg ttgaagatga ttctctcatt atagaggatg
     5101 caaatgataa agaactttcg cagacatttg ttgtatttga catagagaca actggctttt
     5161 ctaatacaaa tgataaaata actgagattg gtgctgtaaa aatagaaaac tttaagattg
     5221 tagatagatt tagtgagtta ataaatcctg aaaaagatat ttcttataag atacaggaat
     5281 tgacaggaat aactaatgaa ttaattaaag acaagccaac cattgaagaa gttcttccta
     5341 agtttatgga atttgttgga gatagtgtat tagtagcaca taatgcagag tttgatactg
     5401 gatttatatc gcaaaaatgt agggaacaag gcttgttata taataacaaa aaagtagata
     5461 cacttatgtt agcaagagta atgttgccaa acttaaaaag acatagatta aatgtcgttg
     5521 caaaagaact tggtattcct cttttaaatc accatagagc agtggatgat gctgaagcta
     5581 cagcacttat atttaataag tttttgcaga tgcttacaga aaaaggtgca aaaactctaa
     5641 gtgatgtaaa caatatactt ggaaagattg attacacaaa gcttagcaca aatcatataa
     5701 ccttgatagc aaaaaattct ataggtataa aaaatttata taaaataatt tcagatgcac
     5761 atgtaaatca tttttttaga gcaccaagaa tccttagaag tgtattaaat gaatataaag
     5821 aaggattaat aattggttcg gcttgtgaag ctggggtggt ttttcaagct gtaaagaaaa
     5881 acgtgtctga tgaggaaatg aaaaaaataa tagatttata cgattatatt gaagtaatgc
     5941 ctatagataa taatagattt atgattgata aaggagaagt aaaggacgaa gaagagttaa
     6001 gggagttgaa taggaaacta attgatacag ccaaaaaatt tgacaaaatt ccagtagcta
     6061 ctggggatgt tcatttttta gacaaacatg aagctgtctt aagaaaagtt cttaaatatt
     6121 ctcaaggttt taaagtagat gaagaagaaa cttatttaca ttttagaaca acagatgaaa
     6181 tgttggaaga gtttagttat cttggagaag aattggcata tgaagttgta gttgaaaata
     6241 gtaatctgat tgccgatatg gttgaagata taaaacctat accagatgat acttatcctc
     6301 caattataga gggctcagat gtggagttaa gagaaatgtg ttacaaaaaa gccaaacgaa
     6361 tctatggaga cccaatacca gaaatagttc aaaaaagact ggatagagaa ttaaattcaa
     6421 taataagcaa tgggtatgca gttatgtata ttatagctca aaaactggtt gcaaaatctc
     6481 tttcagatgg atatttagtt ggttcaagag gctcagttgg ctcatctttt gcagctacta
     6541 tgagtgatat aacagaagtt aatcctcttc cagcacatta tatttgtgaa aacgaagact
     6601 gtaaatattc attcttttat gaaataggtg aatggggctc aggtgttgat ttaccagaca
     6661 aggattgtcc aaagtgtgga agaaaacttg ctaaaaacgg tcatgatatt ccatttgagg
     6721 ttttcttagg ttttgaaggt gataaggaac cagatataga cttaaatttc tcaggaaatt
     6781 atcagccaac aattcataaa tacacagaag aactatttgg agaggggtat gtatataggg
     6841 ctgggactat aggtactgta gcagaaaaaa cagcttttgg atacgcaaga aaatatgtgg
     6901 aagaaaataa tataagtgta ccaaatgcag aagtacttag gctttcaaat ggatgtactg
     6961 gagtaaaaag aacctcagga caacatcctg gtggagttat ggttatacca gactataaag
     7021 atgtatacga ttttacacct atacagtatc cagcaaatga tacttcttgt ggtgttataa
     7081 ctactcactt tgattatcat tcaataagtg gtagaatact taaacttgat atacttggac
     7141 acgacggtcc taccattata aggatgcttg aagatatgac tggaataaat ataacagaaa
     7201 ttcctttaga tgataaagag actatgtctc tatttacatc tacagaagca ttaggtgtta
     7261 caccagaaga aataaactgt cctataggtt gccttgctgt accagagttt ggaactaagt
     7321 ttgtacgaca aatgttactt gacacaaaac ctactacatt tgcagaactt gtacgtatat
     7381 ctggtctctc tcatgggaca gatgtttggg taaataatgc acaagactta gttagagaag
     7441 atatagttgg attaaaagat gttatatcta cacgagatga cataatgaac tatttaatat
     7501 ttaaaggatt acctcccaaa atgagcttta caataatgga gagtgtaaga aaaggtaaag
     7561 gtttaaaacc agaacacatt gaagaaatga aaaaaaataa tgttccagaa tggtatatag
     7621 gctcatgtaa aaagattaag tacatgttcc caaaagcaca tgctgttgct tatgtaatga
     7681 catcatttag aattgcatat tgtaaagttc actatccaga agcattttat gcgacttatt
     7741 ttacgacaaa agttgaggat tttgatgcag atttaattgt taaaggatta gatgctataa
     7801 aatcaaaaat aaatgaaatt gagtcactgg gaaatgatgc aaccacaaaa gaaaaaggta
     7861 tgttgaccgt tttagaagtt gctcttgaaa tgtatgctag aggaataaaa ttattaccag
     7921 ttgatatata taaatcagat gcaactgagt ttatagttgt aggagaaaaa acattacttc
     7981 caccaatggc agcaatacaa ggtcttggag gaaatgcggc tataaacatc caaaatgaaa
     8041 gaaaaaatgg tgaatttata tcaaaagaag atttgagaaa gagaacaaaa atatcaaaaa
     8101 cggtaataga aactcttact aatcatggtt cacttgagaa tatgagtgag agaaatcaat
     8161 tgtcattgtt ttaagttagc ccaaaaagtt gcaaataaaa gaattttatg atatcatagt
     8221 atagaaaata gtatactttg cagaaactaa gagtgagaca gctgttctca ctcttttatt
     8281 ttaaatatat tattaaaaag ctggtttatt tttattaaat atattttatt taagattata
     8341 taaactggtc atttacaatt gaataggagg aactttaatg aaaaaaaatt tagaagctac
     8401 aattgaggag attgtaacta aaataacgga tgaacatgga tttgaaatgg ttgatgttga
     8461 atatgtaaaa gaagcaggag aatactactt aagagtatac atagataaag aagaaggaat
     8521 atctttaaat gaatgtgaat tagttagtag agaattaagt ccaatactag atgaaaaaga
     8581 ccctataaaa gaaaattact ttttagaggt atcttctcca gggcttgata gagcattaaa
     8641 aaaagacaga gattttgtta gatatcaagg tagagatgta gatttaaaat tatataaacc
     8701 attaaatgga tgtaagcaat ttgaaggtga acttgtagga ttaacagaag ataataatat
     8761 aaaaatcatt gcaaatggta aggaaatgga atttaataga aaagatgttg ctattgtcag
     8821 attagcaata aagttttagt ggtatattaa atagggagga tataaaaaat gaatcatgaa
     8881 tttatggaag cattagatga attagtagtt gacagaggta tagataaaga aatacttata
     8941 gatactatag aacaagctct tttaactgca tacaagaaaa actttggttc agcacaaaat
     9001 gttagagttg aatttgatag agacaaggga gatataaaag tattttctca aagagttgtt
     9061 gttgatgaat cagatttata tgatactttc ttagaaatag agttagcaga tgctagagag
     9121 ataaatccta attatgaatt aggtgatatt atagaacatg aagttacacc taaaaacttt
     9181 ggaagaattg cagctcaaac agcaaagcaa gtagttgttc aaagaataag ggaagctgag
     9241 agagaaatag tgtataatga atttatagaa aaagaaaatg aaatagttac aggagaggtt
     9301 gctagagcaa ataaaaatgt tgtttatgta aatcttggaa gaatagaagg tataatgact
     9361 caaagtgaac aaataccatt tgagaattat aaagcaggtc aaaaaataaa attctatata
     9421 cttgaagtta aaaagactaa caaaggacct caaatagttg tatcaagaag tcatccgggt
     9481 ctagtaaaga gattgtttga agaagaagtt cctgaaatat atgaaggaat agttcaaata
     9541 aaatctgtag caagagaagc agggtcaaga actaaaatgg cagtaagatc tatagatgaa
     9601 aaaatagacc caattggagc ttgtgttgga ccaaaaggac ttagagtaaa aaatatagtg
     9661 gatgaacttg gcgatgagaa aatagatata ataaaatata gtgaagaccc agcagagttt
     9721 atatcagcag cacttagccc atcaaaggtt gttagagtag atatagatga agatgaaaag
     9781 tctgccttag taatcgttcc agattatcag ttatcgcttg ctataggtaa agaaggtcaa
     9841 aatgcaagac tagcagcaaa attgactaac tggaaaatag atattaagag cgaatctcaa
     9901 gcagaacaag aaatgttaaa tcttgaaaat tccaaagaaa tagaagctga ggatgttgaa
     9961 gctgaattta tcgaccaaga agagttagat acttcagagg ataattttag tgaagaagag
    10021 taaggaggga tgattttgaa aaaagtaaaa aaagtgcctc aaagaaaatg catagcatgt
    10081 caagatagag actctaaaaa agagcttata agaatagtta aaaataaaga aggaaaaata
    10141 tttttagacc caacaggaag agccaatgga agaggagcat atgtatgtaa aagctctgaa
    10201 tgtctaaaaa aagctataaa gagtaaagca ttaaataaag cttttaaaat agatgttcct
    10261 gatgaagtct atgataatct acttatggag ttagaagaat atgaaaaata ataaagaaaa
    10321 agtactttcg ttcttagggc tcgcaactcg tgcagggaaa atagtgtctg gtgatgattc
    10381 aactttgctt gaccttaaaa aaggaaaagt aaatttgatt ttaatcgcag aagatgcatc
    10441 aaataatact aaaaaacttt ttaaagataa atcaactttt agaaatattc catatctatt
    10501 tttttcaaca aaagaggaaa taggatttgc aataggaaaa tctccaagag cagttgtagg
    10561 aataaaagat gaaaattttt ctaaaaagat aattgaacta atagaaattt aaaataaaat
    10621 agggggtgat cttgtgtcaa aaacaagagt ataccaaata gcagaagaat taaatatatc
    10681 aaatgaagag cttataaata agttagctga attagatata aatgtaactg acaaagattc
    10741 agtattagaa ggtgaagagt tagaattagc acttgaaatg ttaggtgaag atttatctca
    10801 agaaaatgga aatgttatag aaatagatgg aaagttgaca gtacaagtat tagcaacgaa
    10861 attagataaa tctccatcag aaataattat gaagcttatg aaaatgggaa ctatggcaac
    10921 aataaatcaa gagataagtt ttgagattgc tgctttggct gccaaagact atggatttga
    10981 attaacagta gctgaaagtg atgatacaga agctttagaa atagaagctt taatggaaat
    11041 tgaagaagac aaagaagagg atttaaaacc tagaccacca gttgttacag ttatgggaca
    11101 tgttgaccat ggtaagacat ctttactaga tgcaataaga aaaactgatg tgatctcagg
    11161 tgaagctgga ggaataactc agcatatagg tgcttcagaa gttaaaatca atggacacaa
    11221 aatagtattt ttagatactc caggacatga ggctttcaca tcaatgagag caagaggagc
    11281 acaagttaca gatatagcaa tattagtagt tgcagcagat gatggtataa tgcctcaaac
    11341 agttgaagct ataaaccatg ctaaagcagc aggtgtacct ttaatagttg ctattaataa
    11401 aatagataaa ccaggtgcaa acccagataa ggttaaacag gaattagcag accaaggatt
    11461 attagttgaa gactggggtg gagaagtaat agcagttcca gtatcagcta agaaaaaaga
    11521 aggaatagat acacttttag aaatggtatt attggttgct gaaatggaag agttaagagc
    11581 taatccaaat aaaagagcag taggaactgt tatagaagct gagcttgata aaggaagagg
    11641 gccagttgca acagtacttg ttcaaggtgg tactttaaca gtaggtgacc caatcgttgc
    11701 aggtgttgca tgtggtaaag ttagagcaat gataaatgca aaaggtaaga gagtaaaaac
    11761 agcaggacca tctacagcag tcgagatatt aggtctatca gaagttccac aaggaggaga
    11821 ccaatttgtc gaagttccaa ctgataagat tgctagaagt gtagcagcaa gaagacaaca
    11881 aatagttaga gatgaaatgt taaaatcaac tcaaagatta tcactagatg ctctatttag
    11941 tcaaatgagt gaaggttcta taaaagacct taatatagtt ataaaagcag atgtacaagg
    12001 ttcagtacag gcagttaaac aatctttaga aaaattatca aatgaagaag ttcaagttaa
    12061 agtaatacat ggtggtgttg gagctgtaac agaatcagac atattacttg cagcagcttc
    12121 taacgcaata ataataggat tcaatgtaag accagttcca ggtgcagaat ctctaggaga
    12181 aaaagaaaat gttgatataa gaacatatac tataatatac aaggcaatag aagatataca
    12241 agcggctatg actggtatgt tagaccctga atatgttgat gaagaaacag gaaaagctga
    12301 aataagagaa atatataaaa tatctggtgt tggaacagta gctggatgtt atgtgactaa
    12361 tggtaagata tttagaaatt gtaaagtaag attagttaga gatagcataa taatacatga
    12421 aggtgagctt gcagcactta agagatttaa agatgatgta aaagaagtta attctggata
    12481 cgaatgtggt atgagttttg taaattataa cgacataaaa gaaggcgata tagtagaagc
    12541 ttatataact aaagaagtag aaagaaaatt ataaattcgt atcaaagaaa atcagttagt
    12601 aaagaaggtc gatggtatgg catcatataa tagaacaaga aggatagcag aagaaataag
    12661 aaaagtcgta agtactatgc ttataaatgg agtaaaagac cctagaataa catctatggt
    12721 gagtgtaact gatgtagaag ttacaaatga cctaagatat gcttatgtat atgtaagtat
    12781 tctaggcgga gatgaagaat ccacactaac aggtcttaaa agtgctggag gatatataag
    12841 aagagaagta ggaaaaaata taaaacttag atatattcct gagatagtat ttaaacttga
    12901 tgattcaata gaaaaaggaa tgtatatgga tagccttata aaaagagtta atgaaaaaaa
    12961 tgctcaacaa aatgaggatg aaaattatga tgaataatat ggatagtatt ataaactata
    13021 tttatgaggg tgactatttt atagttacct ctcatatcag tccagatgga gacaatattg
    13081 gttctacact ttcaatgtat tatacattga agaatttagg aaaaaatgta tattatgtac
    13141 ttgatgatga agctccatta aatttaaggt ttttggttga aggtgtaaaa atctataagt
    13201 ctaatgaatt taatatgaag aactattctc taattgcact tgattgtggg gataagatga
    13261 gaatatgtgt gagtgatgaa atcaaaaata atgcatcaaa attaatctgt attgaccatc
    13321 atgctagtaa tgattcatat ggagatttaa attatgtaga tattgatgca tcttctacat
    13381 gtgaattagt ttataattta ttagttagat ttcagcaaat aaaagatatt aatattataa
    13441 atgaagatat agcaacatgt ttatatacag gtcttgtaac tgatactgga aacttttcgt
    13501 attcaaatgt acatgcaagt agctttgaaa tggcaaaaaa tttacttgtt ttaggtgcac
    13561 aaaaaaatac tataatacaa aatatatatc aaagcaattc ttctggttat tacaaattat
    13621 taggagaagc tctaaagggt ttggagatat ttgattaaaa aatagttacg cacactcgtg
    13681 actttagtca tgagttagta gctaaaatag tcagcatata gggaaactta tatgtagaga
    13741 taggataaaa cctatccaat actacttgaa ttgctggaaa cccctaaagc taaccaaact
    13801 aaaacataag gatgaaataa acctaagtgt gaaagttacg aaagtagaaa aaattggtta
    13861 gatggtataa ggttaaaccc taagtacttt aaaatgggca atcagcaggt aaactccgaa
    13921 taggagaaac ttcaacgact attcctcttg agggaagtac actacaagct attggtagtg
    13981 gaagtgggta gaccttaacg gataatgccg aaggataaga tatagtctgt gcttacatga
    14041 aagtgtaaga agttcataag agaactgcat aggtagtagc gagcttatgt gaacgacaac
    14101 ctcaaaaatg ataaaagtta agacttacgg ttcttaaata tgttatttaa aattaaatat
    14161 aaaaccttct aaataattgt tttaaataat tacatttgtt aatattatga taggtggtta
    14221 ataaattgtt caccacagca ttatataata tattaaaatc attaaaaagt gtatcaaaaa
    14281 acaattaaag tgaggtgata gcagtgaaaa aagcctataa gtttagaatg tatccaaata
    14341 aaaaacaaca agagttaatt aacaaaactt ttggatgttg tagatttgta tataataaat
    14401 atcttgctaa aagaatagat gtttataaaa gcgataaaga aacttttact tataaacagt
    14461 gtagttctga tttgaccaat ttaaaaaaag aattaaaatg gcttaaggaa cctgacaaat
    14521 tttcactaca aaacgcctta aaagatttag ataatgcata taaaaaattc tttaaagaaa
    14581 a
//
LOCUS       DAMJWU010000092        14523 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMJWU010000092 DAMJWU010000000
VERSION     DAMJWU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.096"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            165..1076
                     /locus_tag="PC460_002877"
     CDS             165..1076
                     /locus_tag="PC460_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2601753.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1251..1748
                     /locus_tag="PC460_002878"
     CDS             1251..1748
                     /locus_tag="PC460_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2601754.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            2492..2854
                     /locus_tag="PC460_002879"
     CDS             2492..2854
                     /locus_tag="PC460_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2601755.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            2919..3536
                     /locus_tag="PC460_002880"
     CDS             2919..3536
                     /locus_tag="PC460_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF2601756.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            3533..4018
                     /locus_tag="PC460_002881"
     CDS             3533..4018
                     /locus_tag="PC460_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF2601757.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            4061..4579
                     /locus_tag="PC460_002882"
     CDS             4061..4579
                     /locus_tag="PC460_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF2601758.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFRVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            4715..5674
                     /locus_tag="PC460_002883"
     CDS             4715..5674
                     /locus_tag="PC460_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2601759.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            5700..7403
                     /locus_tag="PC460_002884"
     CDS             5700..7403
                     /locus_tag="PC460_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF2601760.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            7664..9766
                     /locus_tag="PC460_002885"
     CDS             7664..9766
                     /locus_tag="PC460_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF2601761.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            9779..10219
                     /locus_tag="PC460_002886"
     CDS             9779..10219
                     /locus_tag="PC460_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF2601762.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            10255..11058
                     /locus_tag="PC460_002887"
     CDS             10255..11058
                     /locus_tag="PC460_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF2601763.1"
                     /translation="MIKLTDEEFIVLVNYVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            11098..11694
                     /locus_tag="PC460_002888"
     CDS             11098..11694
                     /locus_tag="PC460_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF2601764.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            11800..12663
                     /locus_tag="PC460_002889"
     CDS             11800..12663
                     /locus_tag="PC460_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF2601765.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            12730..13719
                     /locus_tag="PC460_002890"
     CDS             12730..13719
                     /locus_tag="PC460_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF2601766.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYIEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAINNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            13811..14422
                     /locus_tag="PC460_002891"
     CDS             13811..14422
                     /locus_tag="PC460_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2601767.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         5916 a         1370 c         2688 g         4549 t
ORIGIN      
        1 aataaataaa ttaagatgat aatatccaaa gtgagaaaga taatagaaag taaataaaat
       61 catgtaagaa taatttagat aagattctaa gattttattt atttaaaaat tagtatgatt
      121 aatttagatt aaataaattt aataatttta gtaggtggta atgaatgaaa ataaatagat
      181 taacagaaat aatagtaatt ctattaaata aaaaattagt aacagctaaa gagttggcag
      241 atagatttga agtttctaca agaaccatat atagagatat agaaactctg tctatgtcgg
      301 gagtaccagt atatatgacg aaaggcaaag gaggagggat atctttaata gaagaatatt
      361 ctatagacaa ggctatattg tcaaaaaaag ataaagagag tttaatagtg gcactgaaaa
      421 cattacaggc gactaagtat ccagaaataa attcagtagt aaataaaatt ggctcaatat
      481 ttggagagca gaatttttca aactggattg aaatagactt tactgaatgg ggaagtaatt
      541 ttaatgaaga tgataaattt acaaaaataa aagaagctat tttaaggcgt aatactataa
      601 attttaatta tgtaaatagt ttgtcaagcc agacaaacag aactgtcgaa ccactgaagc
      661 ttatgtataa gagtaagact tggtacttgt atggattttg taaactaaaa gatgatttta
      721 ggatatttag aattagtagg ataaggaatt tatctataaa ggatgaagta ttttcaagaa
      781 agataataga agaagtatgt ttgaatgatt ctaaagtaat taaggaaaat actataacgt
      841 tgaaattgag gtttaaagaa aagatgcttt ttagagtatt tgatgatttt aataaggatt
      901 taataactaa aaatgaagat gatacatatg atgtaattac agaatttcct attggtgaat
      961 ggatttatgg atatatttta tcatttggtg acaatgttga agtacttgaa cctaaagatg
     1021 ttagggataa tgtaataact agactaaggg agttgtctaa aatttattca ttataatata
     1081 atgctagatt aaagatttta ttaaatatat aatatcttgc atttagtata taatatctca
     1141 ctgagtttaa ttatattaaa aatatgacat aaaggtgtcg tatattataa attataattg
     1201 aattagttca aaataaaata tattaataaa aacaaagtga ggtataaagt atgaattatg
     1261 aaatagtaga gataaatgag aaaatagtta taggaatatc gaaggagact acaaataaag
     1321 atggacaagc ggtaaatgat ataggtgaat tgtggaaaaa atttatgggc aaagggatat
     1381 ataatgctat aaagggtaaa aagaatgaca agacaatagg tctatataca gattaccagg
     1441 gtgattttac aagtccgtat agctttgttg catgttgtga agtaaatagt aattctgata
     1501 aagagaagaa tttagaagaa ttaaacacta ataatactgt tggagaaagt attattagta
     1561 aagttatacc agcaggtaag tatgcaaagt ttgttatagt aggaggtcaa aaagaagtag
     1621 gagatttttg gtttgagttt tggcaaatgg attttgatag gacttatata agtgattttg
     1681 aagagtatca atgtaatact tttgatacaa agaaacaaga agttcatatt tatataggaa
     1741 taaagtagat ttgttattac tatattgaat gaagtattga gtatagtaca tttattatag
     1801 atagttttat tatttataac tataagcagt aaatctgtaa aagatttact gcttttttag
     1861 taattaatat tatcagtatc actttgaatc ttcagacata ttcttcattt aatattgttt
     1921 attgataaat gatacttgta aatataatga tttgttattt aatttaaaat atgaaaaaaa
     1981 gtattataaa cacattagaa tacttctgga tttatgagta tacaatttga aaaatataaa
     2041 aaataatgta taaagctaaa tctaatttaa tcgataatac atatagagaa atataattaa
     2101 gtaaataaac aactatctat tagatattga gaaagtcaaa tcctatatga atctacagaa
     2161 atgattttaa tgaatctgaa aaattttttt atacaaacat aaaaaaggta taaagttgtt
     2221 ttattataat ttaattactt ttgataactt tttatgtttt atacaattat atttttaata
     2281 actcgtcaaa acgccaaaca tatattacaa tagatatata agtttttttg ttgacaaatt
     2341 atgttaagag aattaagtat aatatggcat aaataattac ttttatttat caatgaaatg
     2401 tatttattgt gtagcgaaaa atagtcagat agtatagaca ataactgaag ataaaagcgt
     2461 ttattgttgt acatatggag gagttagttt tatggaaaat aaaattttga tagtagatga
     2521 tgctattttt atgagaatga taattaaaga agctctgagt aaaagtggct ataataattt
     2581 aatagaagca aatgatggag aagaagcgtg taatatattt aaatatgaaa agcctgattt
     2641 gatgttgctt gatattacaa tgccaaaaaa agatggttta gaggtgctaa gagaaataaa
     2701 aaagttggat tcaagtgcta aggttgtaat gtgctctgca ataggacaag agaatatgat
     2761 tgtagaagcc ataaaacttg gagcgttaga ttttatagtg aagcccttca aaacagaaac
     2821 attagttaaa gttgtagata atgctttaaa ataaaagtgt aaatatacac taatacaatg
     2881 aaaagcaaat aaatgcaata ataaagggag gggtttatat tataaattat ttagaacttg
     2941 atgatgttca tatagatgct cttagagaaa ttggtaatat aggctctggg aatgcaatta
     3001 ctgcacttgc atccatgata aatagcaatg tagaagtatt aatacctatg gtcaagatac
     3061 ttgaatataa tgaagcgaca aatttgcttg gtggtccaga aaacaaagta gtatgtattt
     3121 tactcgatat gaaaggtgat atcaatggaa tgtttatgtt tttgttggat gaatcaataa
     3181 ctcaacttat gctaagttct ctttttaata aagaagaagc ttttttagat gagattgagg
     3241 caattgaaat ttcagcaatt aaggaaatag gaaatattat ggcaagttca tatgtaaatg
     3301 ctattgcatc tatgttaaac atgactataa gtgtttcaat tccagatata tgtattgata
     3361 tggttggagc tgttctaaat gtacctatga taagattttc agatgttggt gacaaagtct
     3421 tatttataga aaataaattt aaaatgagtg ataattattt tactagtcac attttgatga
     3481 ttccagaaat gagctcactt agagaaatac tcgtgaggtt gggattagaa gtatgaatag
     3541 agagatagta gtgaacattg cagatatgaa gatagcctat agaccaaatg ttttagttac
     3601 ttatgcactt ggttcttgtg taggagtatg tttgatagat aaagtagctg gaattggtgg
     3661 aatgttacac gttatgttgc catatagtaa agatgctatt aacattgaaa ataagtgtaa
     3721 atttgctgac acaggaataa atgagctcat taaatctatg gagaatatag gtgcaaatag
     3781 aatatatata aaagcaaaaa ttgctggagg tgctcagatg tttttaggaa gtagcaattc
     3841 agtaattgca agtataggac ataggaatgt tgaggcagta aaaaaaactc ttttaggatt
     3901 aaatattcca atattagctg aggacacagg tatgaattat ggtagaacaa tacgttttta
     3961 cactgagacg ggtgaattac ttgtagattc aataaataaa ggtaagaaga aattataata
     4021 tatatttcat agaaagtgta ttttaggagg gaggtattaa atgagatttc cagatgaccc
     4081 atatttagat tatggactaa ttgatgaagg aattaagtat ctaaagttta gagtagatga
     4141 gcaagacttt ggggttgaac ttgaaaaggt aatagaaatt actggaaatc aagaaattat
     4201 atttattcca gaactaccta catattctaa aggcataata aatctaagag gaaaaatcat
     4261 tccgataata gacatgcgtt tgagacttaa aaaagatgaa ttaatgacta caaattgcat
     4321 gtatatagta gtaactgaaa taaaggactt ggtagttgga tttgtggttg atagagttga
     4381 tgatatagta agcttagaaa aaagtaaaat atcacctcca ccaagagtta ctatagaata
     4441 ttcggattat tttgtaagtg gagttggaga attagaaggt aaaataatta ctttactaga
     4501 tttagagaaa cttttgacag ataaagatga agttttaata gatgaattaa tcaatgggtt
     4561 tgattgcact atcatgtaac atagggatgt agttggagtg ttattataaa aagattgtga
     4621 tgagaacatt taagaatata gtggtataat tattaagtat aaaaatttta aatcattaaa
     4681 atttgaatat attatgttta agagaggttt gtatatgaaa aataagcaac agggaacaat
     4741 tttagtcgtt gatgatagtg ttctgatgtg tgacttaata gataaaacac ttacaaaaga
     4801 taattttaag gttaaaaaag catttaatgc aatagaggca aaagaattta ttgaagaatt
     4861 tataccagac attattttgc ttgatataat tttaccagat aactctggtt ttgagttttg
     4921 taaggaagta aaatctaatg ctcgtacagc agatatacct ataattttta taacttcaaa
     4981 aaatacagat acagacatag taaaaggatt tggtgtagga gcaattgact atatggtgaa
     5041 accttttagt atgacagaat taaaagctcg tgttatagct catcttgaag taaagaaatc
     5101 tcaagataaa ttaaaaaaga taaataatga actggaatct tctcttgaaa aattgaatag
     5161 actcgttgta agagattatc ttacaggtct ttacaatagg cgacatataa ttaatcagct
     5221 tatggagcaa agaagaatta ataaatatag tgaaacatca atatccctta tccttggtga
     5281 tattgatgac tttaaggtta tcaatgatac ttatgggcat gaagcagggg atttcgtgtt
     5341 aacagtaata tcaagtataa taaaaaagaa ttgtagacaa attgatactg tatcaagatg
     5401 gggtggagaa gagtttttga taatgctcac aaatactcca atagaaggtg ctaagatact
     5461 ttcagaaaga atccgtaaag agataaagga gtttgatttc aactataaag aacataaaat
     5521 aaagtgcact attacattag gagtagtaga agtaaactca aatatatcat tagaagaaaa
     5581 catatcctta gcagataaag caatgtatga gggtaaaaac ttaggaaaag attgtagcgt
     5641 agtgagtaca atgaaaggat taaaaaagat ataaacttac tttatgaagg agtacagata
     5701 tgaaaagaga agtaaagctt tctaaattag caattgtatt gctgattatt atagtattgg
     5761 ctgttctagc agggattttt tcttctattt catcttataa agcatttgaa aaagtagaaa
     5821 aaagtattaa tgatatgaat aatttgagga ctcaaagtca gtatataaaa gatacaacta
     5881 gagagataag tgatgtatta agaagatatg tatttacagg agaaaaaaag aataaagata
     5941 tttatgaaaa agaattatac ggaaaaacaa gagaatcagc tttaaacaaa ttagaaaaaa
     6001 ttggtcttac aaagagcgag tcaaataaaa tcttagaagc aaaagaaata tcagattctt
     6061 tagtaccaac agaacaaagt gcaataaagg cagtcgaaag taatgataag agtaaagctc
     6121 aaagtataat ttttggtagt gactatataa actcccaaga gaaaatggat tctttaataa
     6181 atgaatttca agaggatata gttacccgtt caaattccaa aattgtagag gtaaaaaaag
     6241 aattacaaag tctaatatta agaacaatat ttatactttc ccttttaata atattgacaa
     6301 taatagaata tacagtaata aaattaaaaa tagtgattcc tattaataaa gtagaaaaac
     6361 acttttcaag aattgcatct ggggacttaa gaacaagtat agacgttgaa gaaagtaaat
     6421 ctgaaatagg gctattagtg agttctgtca aaaagatgca aagtatgctt ttaaattaca
     6481 tagaccttat agacagtaca ttaactagta ttgcaaaagg agatttaaga gtagagatag
     6541 accaagaatt tataggtgat tttaaatcaa ttaaaatatc tttagaaagt ataataaact
     6601 cttttaatgg tgatttttct gaaataaatt tatcagcaga gcaagttgca agtgcttcag
     6661 aacaagttgc agcaggtgca caagctcttt cacagggtgc tactgaacaa gctagttcag
     6721 tagaagaatt agcttccacg attaatgaga tatcagatgg aattaagaag tgtgcagata
     6781 gtgctagtct tgcatgtgag gtatctgacc aatctgcatc acaagttggt tttggaagta
     6841 gatgtatgga tgatatgatg acatcaatgg gtgaaataag tgaaaaatcg agtgaaatat
     6901 caaaaataat caaaacaata gatgagatag cattccaaac taatattctt gcattaaatg
     6961 cagctgttga agcagcaaga gcaggtcaat atggtagagg atttgcagtt gtagcagatg
     7021 aagttaggtc tttggctggt aaaagtgcaa aagcagctca aaatacagct attctcatag
     7081 aagaagctat taaaacagta gaaaatggtg tcagaattgc taaagagact gcaaatgcat
     7141 tggacttggt agtaaatggt gtagaaaaaa ctactaatta tattaatggt atttcttcgg
     7201 ctgtacatag tcaagaagta gcaattacac agattttatc tggaatagag caaatttcca
     7261 tagtagtcca aatgaactca gcaacagcag aggaaagtgc agcagcctca gaagagatgt
     7321 ctgctcaagc tcaaatttta aaagaattag tagaaaaatt tcatctaaag gaaagtgaag
     7381 taagagaatc atcatgggtt taaattatga ggtgaatatt gaagtaagag aattatcatg
     7441 tatttaaatt atgagatgta tatatattga aataagagaa ttatcatgta tttaaattat
     7501 gagatgtata tatattgaag taagagaata attgtgtatt taaattatga ggtacatata
     7561 gtgagataag agaatcatca aggatttaaa ttatgagatg catatataat agaaaattat
     7621 ttgtaggtcg taggtgataa atgtgaatta tattgaaccg agtatgagac aaatgttaga
     7681 tatgtttata tttgagacaa gtttattact cgaacaatta gatgaaatta tgattcaaat
     7741 agaaaaagag aaagtattta ctcataacaa cattaatgaa acttttagaa taatgcatac
     7801 gataaaaggt tcagcatcta tgatggggtt agacaatata tctgctttag cacatagtgt
     7861 agaagacttg ttttatgcta tacgtgaagg taaaatagaa gttttagaca atgacctatt
     7921 atttgattta gtctttcaat cattagattt tataagaaga gatatagatg agttacaatc
     7981 aagaggttgt atatcttctg attactctaa ttttattgaa ggaattgaag agtatgtgag
     8041 tactttggaa ttatcaagtt ctatgaaatc aaaggataat gcaaatttag aaagtaaaga
     8101 cgaaaaagaa gtaatcgatt caaattttga atacaaagaa gactctgatg tagtaaaaat
     8161 aatctttgaa caaggttgca tgatggaaaa tgtgagagct ttcatgcttg tatttgaact
     8221 tagagattac tgtgaattta tagattttta tccaaaagat gtagaaacaa atgcagagtc
     8281 tacagagtat attaaaagtt atgggtttta tatacaattt gtagctaaag aggataaggc
     8341 aatagtttat aaaactatag aagataatct ctatgtaaaa gaatacataa atattagtaa
     8401 aaaagaaata gaaatgaaaa atacatcaaa caatgatgca gtagaaaata aattaaaaga
     8461 taataataaa gtaagtaaaa gttatcaaaa taactataca gaagatgaga tacaagaaga
     8521 tgttttaaat gaaaaaactg atatggaaaa agatagtcta agtgaagaaa atgaacattt
     8581 accaataagt aagcagagta ttatgagtgt aaatttagaa aaattggact tacttcaaaa
     8641 tatagttgga gaaattgtaa ttatggaatc aatggttgta aatagcgtaa attctacagg
     8701 tgttgatata gataatttca ataaggcaac aagacaatta cacaagctta cagatgaatt
     8761 gcaagatata gtaatgtcta tgcgtatgat tcaaattgct ggtgtatttc aaaaaatgca
     8821 tagaattgta cgagatatga ataaaaaatt agataaaaat gtagagttaa taactatagg
     8881 agcagagaca gaggtagata agagtgtaat tgataattta gcagaacctt ttatgcacct
     8941 tataagaaat gctatggacc atgcaataga gcctattgag gagcgtatag cgaaaggtaa
     9001 agaagaagtg ggaaagatag ttttatctgc tgaaaatcta ggcggagaag ctgtgataag
     9061 tatttcagat gatggtagag gcattatgag agataagatt ttggcaaaag ctaaggaaca
     9121 aggtcttctt aaacatcaaa gtaatgagta tactgatgac gaggtcaatt ctttgattat
     9181 gactccaggc ttttctacaa aagaatctat aactgaattt tctggaagag gagtaggcat
     9241 ggacattgtt cgtaaaagta ttgaaaaagt tggaggttca attgaagtaa aaagtaaaca
     9301 agatgaaggg acaaccttta ctataaaaat accattaact ctttctatag tagagggtat
     9361 gaagtttaaa gttggagatt cactatttac tttaccaata tcatccataa gaagaacatt
     9421 taaacttaca gacttaaatc aaataattac agaaggaaat aagacagagt taattacaat
     9481 atatggagca tgtattccca ttataaggtt acataaaata tataatattc aaacaaaagt
     9541 aaccaatctg atgaatggta atatgataat tattgaaaat gagcataaag cagtatgcat
     9601 ttttgttgat gaaataatag gtaatcagca agttgttgtg aaaccatttc cagtttattt
     9661 taaccgattt aatataaaaa ataagggttt atcgggatgt acaatacttg gggatggaag
     9721 tataagttta attatagatg cagatagcat tatagaaata tattagggga tttaaagtat
     9781 ggctaaagat gaaattatta aacaagtatt aacattttat gtcaatgatg ttatttatgg
     9841 aatagaatta gaaaatgtga ttgaaaccat aagatttcaa tcaataacgt atgtaccatg
     9901 tcttccaaca tatataagtg gagttattaa tttgagagga agaataatac cagttattaa
     9961 tatgcatata aagtacaatc ttcctaaagc tgattataat gagagaactt gtataataat
    10021 tacaaaggtt gatgagtatc aagttggaat tattgtagat aaagttgttg atgttattca
    10081 tatagaaaat ctaaatttac tagagacgac taattctaac aacactaata ttaataaaga
    10141 tataaaagag attgcgaaag ttgaagataa ttctatatta attttagata ttcgaaagtt
    10201 tttgattaat agcatatagt tagttctgat aaattagcag gaggaagttt ttgcatgata
    10261 aaactcacag atgaagaatt tatagttctt gtaaactatg taaaaaaaga atatggtatt
    10321 gatttaagta aaaaaagagc gttaattgaa ggtcgcttat acaatacaat gatagagaaa
    10381 aaacttagtt cttttagtca atatatgaat ctattattta aagataagac aggaaatgaa
    10441 gctataaacc taataaatag attgagtact aaccacacat tttttatgag agaacctcaa
    10501 cattttgagt ttatacagaa tagtatactt cctttttggg aagaaaataa taaaataaga
    10561 aacataaata tatggagtgc aggatgttct agtggagaag aagcgtattc aatagctatg
    10621 actttggatg attattttgg atataataaa gagttgtgga acattaaaat catagccaca
    10681 gatatttcaa ctaactcact tgaaaaagct aaaagaggta tatacataga atcaaatgtt
    10741 aacaatgttc ctgagttatg gaagaagaag tattttatag acaacaagga tggtacattt
    10801 aaaatttgtg ataagattag aaaaagtgta atttttaagc cgtttaattt aatgaatgat
    10861 ttctcatatc aacattttga tttgattttt tgtagaaatg ttatgattta ttttgattta
    10921 aaaacaagag aagaacttat aaataaattt tataatgcta ccaaaaaaga tagttttttg
    10981 tttgtaggac atgcagaagt aatcaacaga agtagtacta aatacaaata tgtaaaacct
    11041 gctatatata aacgataaaa aggtattttt gatatatgat tttttggagg cggtaatgtg
    11101 gaacgtttta ataatatggt tattgctata ggcgcatctg taggtggtac agaagcaata
    11161 cttgaaatta taaaagacct accaaaatca acaccaggga ttgttgtagt tcaacatatg
    11221 ccagctatat ttacttatat gtatgcacaa cggcttgaca agcagtgtat tatgaatgta
    11281 agagaagcta agaataatga tagagtcgaa caaggaaatg tgttaattgc acctgggggt
    11341 taccagatga aattgtgtac agataaacaa ggatattatg taacttgtga aaagggagaa
    11401 agaataagtg gtcactgtcc atcagtagac gttttgtttg attcagtagc agaagtagca
    11461 ggtaaaaatt caataggaat aatacttaca ggtatgggtt atgatggagc aaatggactt
    11521 ttaaaaatga aagaaaatgg ttcatttaca attggacagg atgaaaattc ttgtatagta
    11581 tatggaatgc caatggtagc ttttgataga ggttctgtta tgttacaatt acctcttggt
    11641 gaaatttcaa actgtttgat aagacatatt aatattttaa aacaaaaaga ttaatttttt
    11701 ataaataaaa attaatcttt tattaaatta tatggataaa aaaccctaga aactccaaat
    11761 ataataagag agatttatat ttaggaggaa aatgataata tgaaattttt taaagtcgat
    11821 acaaaagcta aagttttaag atatgtattt gttttaacat tgactataat ggctgttttg
    11881 gcaattaaac atgactataa atataataat ttaaggaaag atgcttcaat taatttaaat
    11941 agtaataagg aagcagtgac tgttatgagt aaccagtcat taccaaaggg taatacaaat
    12001 agtaaacaaa ctttatcagt aagtacaata ggaagtaaaa caatagtgaa aaatgatgag
    12061 tatttagaaa gtactataaa catgcctgta ataacaaatt caaataaaat tgttgaaagg
    12121 tctacaaatg ataagataaa aaatgacata tttgaatttt ataataaatc atataaagaa
    12181 gcaaagcagt atcttaagga taatcctgac gagaaaaata agtttgttgc aaatgtagac
    12241 tttgaactta aaaagaatac tgattctgct cttagtataa aagtgagata ttatacttat
    12301 agtggtggag cacatggatt ttatcaagat atagcatata atgtggatat gagaactgga
    12361 aaatttttag aattaatgga cttgtttaag gataatacta aatataaaga agttatagat
    12421 gaagaaatta aaagacaaat agctgaactt gaaaaaaagg atgaagaaaa tatcggaata
    12481 tataatttta aaggtataaa agagaatcaa aacttttatt tgcaagatga gaatctagtt
    12541 gtatattttg atttatatga tataactcca tatgcagcag gaataccaga attttcaata
    12601 aataaagcgt tgataagttc tatgctaaaa ccagagtatg tagatttatt tgatttgaaa
    12661 tagaagatta aattaaatgt tatgctataa tatttagcat agagacttat taaaattggg
    12721 ggtataaaaa tgctactttt aaaaaaagac gacataaaaa aagtatttac aatgagagat
    12781 gcaattgaag ctgataagga agcttttaga atttattgtg aaggaaagag tgtaaatcct
    12841 ttgagaacca atatatctgt tccaagtcaa gatgctagta tgttatttat gccaggatac
    12901 atagaagaat taggctgtgg gggaataaaa attgtatctg tatttcctaa aaatgctcaa
    12961 aaaggcaaac cagtaattcc agcaacagta ctattattag atggagaaac tggagaagtt
    13021 agtgcagtat tggatggcac ttatgtaact cagattcgta ctggagcagc ctctggagca
    13081 gctatagacg tattggctaa aaaagattct aagattggag ctttgatagg tctaggagga
    13141 caaggtgagc cacaacttga agccatgata gaagctagaa atttggatga ggtaagagtt
    13201 tttagtagaa acaaggaaag tagagaaaaa tttgcagaag aaatgaactt aaaattaagt
    13261 aaatataata ctaaaatagt tgcagttgaa tcatctgatg aagccataaa taacgcagat
    13321 gtgatagttt tagctactcc atcaaaacaa ccagtactta atggaaactt agtgaaaaaa
    13381 ggagctttaa taagtgcagt aggttcatat atgccagata tgcaagagtt gtgcccaaac
    13441 tgtttaacta gagcgtctaa gatatttttt gaatcaacag atgcagtttt atctgagtca
    13501 ggagatatat taattccatt aaaagaaggt aagataagta aagatgattt tagtggtgat
    13561 ttagggaatg ttataaacgg aactattcct ggaagagaag atgatgatga gataattgta
    13621 tttaaaactg ttgggatagg tgtacaagat gtcgtaacag caaaaagaat atacgataaa
    13681 gcaaaagaaa acggtattgg aatagactgg aagtggtaaa atattcaaaa taattctata
    13741 gttagacaaa tttatgttat aatgttgata aataaatttt ttattatttt aattgaggga
    13801 ggatgtttgt atgaaaatgg taaaaaagtt aatattatct acgattatgg ctctagtttt
    13861 agttctaact atagggtgtt ctaatgcaga agaaaaagta aagtcgaatt ttgaagctaa
    13921 tttagctgca ttagaaatgt ataagtatga tgaaaaagga atttctgatg cattaacaga
    13981 agctgaatat aaacagattc aaacttataa cagcaaggac aaggtttcaa tgattgctca
    14041 attgacacct ctttatggta aagaaaatgc tgaaaagata tacgaaacaa ttgttaaatt
    14101 aattcaaggt gttgaaaaga cagtaacagt ggaatcttct agtaaggata agataacttt
    14161 gaaagttgta agtaaagctt tagatactag taaagtaatg agtgaagcga acactttagt
    14221 aaaatcttat gtaactgatg aaaagaatta cgataaaaaa gaaaaagaag ttcaagatga
    14281 tatgacaaaa atgattatag acctttacaa taagaaacca actaaggaag ttacaactat
    14341 gattacatat actaaaaaag atggtaaatg gcaaccacct agtaatatac aaaagttatg
    14401 tgaaggaata tatacaattt aatttatttt aaagacacaa aactatcttt taagcatata
    14461 ataaaatgtt tggaagatag tttattttat tttcaaagta taaattatat tttataaatg
    14521 att
//
LOCUS       DAMJWU010000093        14503 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMJWU010000093 DAMJWU010000000
VERSION     DAMJWU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.097"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            59..793
                     /locus_tag="PC460_002892"
     CDS             59..793
                     /locus_tag="PC460_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2601768.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNALMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(936..1427)
                     /locus_tag="PC460_002893"
     CDS             complement(936..1427)
                     /locus_tag="PC460_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2601769.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1776..2159
                     /locus_tag="PC460_002894"
     CDS             1776..2159
                     /locus_tag="PC460_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601770.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            2667..3050
                     /locus_tag="PC460_002895"
     CDS             2667..3050
                     /locus_tag="PC460_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2601771.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            3052..3402
                     /locus_tag="PC460_002896"
     CDS             3052..3402
                     /locus_tag="PC460_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601772.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFVDKK"
     gene            complement(3503..3961)
                     /locus_tag="PC460_002897"
     CDS             complement(3503..3961)
                     /locus_tag="PC460_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601773.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(4006..4794)
                     /locus_tag="PC460_002898"
     CDS             complement(4006..4794)
                     /locus_tag="PC460_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2601774.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(4933..5370)
                     /locus_tag="PC460_002899"
     CDS             complement(4933..5370)
                     /locus_tag="PC460_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HDF2601775.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            6192..6701
                     /locus_tag="PC460_002900"
     CDS             6192..6701
                     /locus_tag="PC460_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601776.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            7115..7546
                     /locus_tag="PC460_002901"
                     /pseudo
     CDS             7115..7546
                     /locus_tag="PC460_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(7733..7926)
                     /locus_tag="PC460_002902"
                     /pseudo
     CDS             complement(7733..7926)
                     /locus_tag="PC460_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            8028..8534
                     /locus_tag="PC460_002903"
     CDS             8028..8534
                     /locus_tag="PC460_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2601777.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSALDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            8539..8815
                     /locus_tag="PC460_002904"
                     /pseudo
     CDS             8539..8815
                     /locus_tag="PC460_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
     gene            9084..9656
                     /locus_tag="PC460_002905"
                     /pseudo
     CDS             9084..9656
                     /locus_tag="PC460_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            9759..10574
                     /locus_tag="PC460_002906"
     CDS             9759..10574
                     /locus_tag="PC460_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2601778.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            10655..11131
                     /locus_tag="PC460_002907"
     CDS             10655..11131
                     /locus_tag="PC460_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2601779.1"
                     /translation="MTTYKVNSIGTICNNEKGAFIQLEAPYILALRALDGFSHINVLW
                     WFSDFDNTNFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSAVEIIDIDYMSGLI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPQVPDWCSHWPHSLEESASFAWESEFNF"
     gene            11145..11708
                     /locus_tag="PC460_002908"
     CDS             11145..11708
                     /locus_tag="PC460_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2601780.1"
                     /translation="MSLQDYIFEKPVIETEHLILRTMNKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPELLFDKSKKSTKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKHYQGNGLMSEVLKNVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITRWCYG"
     gene            11963..12874
                     /locus_tag="PC460_002909"
     CDS             11963..12874
                     /locus_tag="PC460_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2601781.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKR
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     SEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEIWIIPV"
     gene            12952..13386
                     /locus_tag="PC460_002910"
     CDS             12952..13386
                     /locus_tag="PC460_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2601782.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            13854..14503
                     /locus_tag="PC460_002911"
                     /pseudo
     CDS             13854..14503
                     /locus_tag="PC460_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         5248 a         2020 c         2422 g         4813 t
ORIGIN      
        1 atattttgta tttaatagag tgttgctaag tgaatatatt taaaatggag gaaactttat
       61 gaaacaaaat aaatatgatg atgatagatt ttttaataaa tacagtaaaa tggaacgttc
      121 tacaaatgga ttagcaggtg ctggagagtg gcatgtttta aaaaaaatgt tacctgattt
      181 caaaggaaaa agagttttag atttaggatg tggatttgga tggcattgtc aatatgcagt
      241 tgagaatgga gcaatttctg ctgttggagt tgatatttca gaaaaaatgt taaaagaagc
      301 tagaaacaaa acgcaacttg ataatataaa ttacatatgt atgcctattg aagatattaa
      361 ttttccaaaa gatagttttg atgtagttat tagttccttg gcatttcatt atattcaatc
      421 atttgaagat atatgtaaga atataagtaa ttgtctttca aataaaggta attttgtatt
      481 ttctgttgaa catcctatat ttactgcata tggaagtcaa gagtggtact gtgataaaga
      541 tggaaataat ctacactggc cagtagatag atattttgaa gaaggagtac gtaggtcaaa
      601 ttttctagga gaagaggtta taaaatatca taaaacatta acaacttatt taaatgcgtt
      661 aatgaaaaca ggatttgaaa tattagaaat tattgaacct caaccagaag aaaaactgtt
      721 agatacaatt cctgatatga aagatgagct acgtagacct atgatgttac ttgtatctgc
      781 aaaaaaaaga taagtaaata taaataatca ttattgtaga aaaaaagttg tagaaaataa
      841 ttacactaat ggagatgatt ataccagtgg tattaattac accagtagta aggaggcaaa
      901 agcattcgtt gcttttgcca ttttttaagt tgcaattata cttctacgta gtatatttcg
      961 tttccatttt cttctataag ctttttcata tcttcaaatt ttaaaaatac agtagcagta
     1021 ttatcatttg gatgtatacc tattttatcc atttttaata aatccttatc aaatacaact
     1081 tttacaacag cctctttatc atttaatata ccaaaaggag tcacagctcc tttagtaagt
     1141 ttcaagtatt tttgaagtct ttcttctgat gcaaaactaa gaggtttact atcaatttgt
     1201 tgacgaatat cttttaaatc tgcttttttg tctttaccta atacaactaa aaaatgttct
     1261 tttcctttag catcacgaag aaataggttt tttacaacaa attccatatg taatgttttt
     1321 aattcttcca tttcttcaat tgtataaact ggtggatgat ttacagcctc ataaggtatg
     1381 cttaactcat ctaatttttt atatacttcc tgttgttgat tgttcatgat ttcctcccat
     1441 tagaaattta ttttatatat aaataatatt atcacagaaa actttaaaag aaaacgtttt
     1501 atataatata gacaatagat tatattttta taaagaagca agaatactca ataaaactta
     1561 aagaaattaa acgatttcta aaaatacctt gaaaatagaa ccttatttgc catttataaa
     1621 ttatataaaa agggattata taaagtcata tatgaaaaaa ctatattata ctatcttttc
     1681 tttttggaat aaactatact ttaaatatag atatttaatt taaataaatt ttacagtatt
     1741 aatagtaaat tatcttagaa aggaatgtgc tttatatgtc tacagacaaa gttgttggaa
     1801 tcatagatga agaaacggca gaacttgcag gaattgaata tactggtaaa atttatgctt
     1861 cttctggtgt tataaaacat ataaagaaaa aacatagatg tcaactgagt aaggatattt
     1921 ttaatgatat aatagacact ataaaaatgg tattaaagtc acctgaatat ataggaagtc
     1981 atcctaaaaa acctggaaaa agtgtggagt ttataaaaaa aattgatgat tatattttag
     2041 tagctacaga attagataca aaaaaaggat atttatatgt atcttcaatg tatgctataa
     2101 aagaagcaaa attagaaagt agagtaaaca gtggaagagt aaaacaatat gatggttaac
     2161 ataatattat taattttccc taaaatattg aaaaatatta ttatataagt tataatatta
     2221 atatacacgt agatatttat tagttagatt tgaggttgga aaaggcaccc aatacgctac
     2281 tatagagtag ttaatagaga tgcaggatac gccgccctgc caaatctaat taaattaaaa
     2341 agagaggatt aattcctctc ttattgtttt tttgagatac taatttaatc tataatagaa
     2401 agattgaagg tcgtttatac aaaaaaattt tcagttatat acattaaact aattaaataa
     2461 gatatttata atctatatgt gatacatatt atatattaat aaaagaaaaa tatatagaaa
     2521 atccttataa aaatttaaat tgtaatgaat aggaagtaat taaaaagaat ttttattgat
     2581 ttttaaaaat ttagttagaa actatatcat tatggtaaaa aatgtattat aatgtttaga
     2641 aatattataa taaggagcga ttaaacatga atctatcgaa gaaaatagaa gataaaagaa
     2701 aagctaaaca gagagctgaa aaagttaaga aagctaaaat tgctacagct ggtgtggtat
     2761 taggagctgt tactggtgca gtgagtggag tacttttggc tcctaaatct ggaaaagaaa
     2821 caagagagga tataaaagat gcttcacaac aaattgctga aaagataaat atgaaaactg
     2881 tagatgtaaa aggcaaagta tctgagaagc tagaggataa aaaaggaaac tttatagaat
     2941 caaagaaaaa aataaaaaaa tatatagata acaaaaagag tgttgaaaat tctagtgagg
     3001 aagataaaat aatagatata gttgaaccag ttagtgttac tgaggaataa tatggacaat
     3061 acaataacga ttaacataag tataatatat gtattattag ctatacttgc ttgtattgct
     3121 ttagtttatt taattattgt acttgttaaa ctacataaga ttttaaataa tgttggaact
     3181 atgttaaatg aaaatactca aaatataaat acaactttaa ataatcttcc taatgttgta
     3241 gaaacttttg gagaagtagg agaaaatatg aaagatgtaa cagaagtagt tactgaagtt
     3301 acagcagatt ttcttgttac taaagacagt gtcaaatcaa atatggatgt tatagttgat
     3361 atattgacta taattaaaaa catttttgta gataaaaaat aataagttaa tataaaataa
     3421 gcctataaga tataatttaa acgaccttaa aatagcattt ataaggtcgt tttttattag
     3481 cttgatttga cactatgtgt ttttaataat tggtaatatt cctctttatc atttgaacat
     3541 atatatctat gcttgttgtt tcaattacat ccataagaac atctttacat tcaagctcaa
     3601 aatcttccat tccttctaaa tattcatcat cttcatcaaa aaagatataa gaattatata
     3661 gccattcttc aaatttttct tcaagtatat cccaatacga actccaaaat aatccacaac
     3721 ctgatatata agatgctcta ctttgattta tataaacttc atttagaagc ttttgtaatc
     3781 tactgctata aagaaaatct gctcttccac tgcttgatat taattcattt aaatcttcta
     3841 ttgacattaa ttcaacttta ccacttttat cataaggagt ataattttca tcaacccatt
     3901 tatgtggttt gttttctctg catactgttg ctatcaactc tacaactttt gttcttatca
     3961 tttttttatc ctaacttatt tattttttat aaaataatca tgattttata atttatctat
     4021 ttcttgtaat gctaatttaa cagtttcata tctatcagca ccatttatta ctacatcacc
     4081 ttttacaagt ttattagctt ctccacctac aacataaacc tttttaccta aaccttgctt
     4141 ataactagac gcttcaatta ttaaagaatc tttcaattgc caatgtaaaa tctgagcagc
     4201 cactttatca gcatcacctg tataaacgat tacattttca tacactttat tttcccctcc
     4261 cccttgagaa tttgatatag atttgtttag tataccttct gctatcaatt tagcaacttt
     4321 atctttatta ttaacataat aatctgtatc tgctttacta tctacaaaac atacttctat
     4381 taatattgct ggtgcaatag tatgattaag ccaataaaga cctctaacat cagactttgc
     4441 acccctatct ttgaatactg tagctaacct agtattcact ctttctgcat aggttttgcc
     4501 attattagtc ttatatattg tttctgtccc tactggagtt gaagttgtag catttgaatt
     4561 aaaatgaatc tgaacagcca aatcagtttc ctgtttattt gctatagcac attggtctgc
     4621 taaatgatta cttgattcat caactcttcc tgtataaaca gtagctccac ctattttaag
     4681 ccatttaaca attaagtcat tcagtattct tgtttctttg ctttcgttta tatacccaac
     4741 agctcctgta ccttttccaa ttaaagtgtg ccctggtatt attactactt tcaaactaac
     4801 atctccttta caattaaatt taacttctat ttaatatatg cataacctag aattttgttt
     4861 ctttttatat cttatacttt tattataacc cattttgtaa aaatatagaa tattaagtaa
     4921 ataatgttaa aattataatt ttgtatataa gacattactt gattgcaata attttatagc
     4981 atattcatct attctatatc cagttctata atatatatgt ttaattccag cttgaattat
     5041 agccttagta caatttaagc atggaaagtg agttacataa atagagcaat ttttaactga
     5101 aataccttct tttgcacaat aataaagggc attttgttca gcgtgaatgg ttctaataca
     5161 atgtccatct ctcatttcat gaccaacttc actacaatgc ttatctccag aaatactacc
     5221 attatatcca gtagacacta ttctgttttc tgaattgaca attatagctc ctacataagc
     5281 tctatcacaa gttcctcttt cagctactgt ttcgcacagt ctcataaaat attcttgcca
     5341 tgagcatcta ttttcaagtt ttttattcaa atcatatacc tccaaaattt atatctaata
     5401 tataagattt ataccttaaa aataatatta aaaacaaatt tatatgataa ataaaattta
     5461 ttaatactgt ttttagtcta aattatttca ttttaaatta aagttacaag gaatctaaga
     5521 ttatatatat ctttcttcgt aatatgaatg ttacaatata aaaaactgtc tcaataaaat
     5581 agagacagtt ttacagtcaa agtttataat acaagttcat atttttcttt aaattctttt
     5641 acagcttcgg gccatgcctt ttgttctgta ataccttttt ctttagctat tttaattgca
     5701 atctctctta tcattgcagc gccttctaat attcccattt ttaatctccc ttcaaatatc
     5761 tttttgtata gaatataaat agtgctaaga tttattacat gctgcatata caatatctat
     5821 acaacacata acttgtaaat tccttttatt ttcatacgtc tttaaccgac atataattat
     5881 attcaataag ttttaaaatt atgaactcaa ttataattat ttttattttt ttagaatcta
     5941 aacactaaaa attgtaggta aatttgtctt atgcctttat aaatatttct acagttttaa
     6001 ttactggaca tatctatttt tattataaaa agcgattaaa aatggagaat atcctcataa
     6061 aaactatata taagcaaaac ggttaaagga aaaagaagaa atttacttga atgttaaaat
     6121 gcgttgcttg aagcaatggg cagttttgcc tataatatcg gtaacaactg aaagaaaggt
     6181 ggttagccct aatgttaagc gaaaacatta aaacaatcag gaagtcaaaa ggactttcac
     6241 aacaagaact tgctataaaa ctgaatgtag tgcgacaaac aatatctaaa tgggaacaag
     6301 gactgtcggt tcccgattct gatatgttga tttccatatc agaggtgctt gaaacgcccg
     6361 taagcacttt acttggagaa actattgttg agtcggaagt tgataactta aaagtgattt
     6421 ctgaaaaatt agagattata aacttgcaac ttgcacaaag aaaaaacatg agaagaaaaa
     6481 tgtttcattg gttatttatt ttattatgtg cagtcatatt aatagttttt gtggtcttag
     6541 ggagactaaa tagtccttac ttggattgga attataccga ccctgaaacc gctgtttttg
     6601 gagtagcttt tcatgctttt gagtggctat ttgtcagatt agtaccgatt gtccttatag
     6661 gagcaattat tggaatcttt ttgacacgta agaaaatatg aagtaactct gctttttata
     6721 atacagcaag cgttccttaa aagcgtcatt ctacatggca atcgaggacg actatgtaag
     6781 gaagaacctt tttgatttcc aactaagcga agttattgag gacgacagta ggcccaaaac
     6841 cgcattaagt aaagtacagg aagcaagact gctggactta tactctcatg cgtcagatgc
     6901 aggaaatgcg aggtctggta gcatgattta caacgatttg tacaacgact acgagtagaa
     6961 atatatggag ataagtatta tctacgaaat cagaaaagaa atgacttgat aatttactca
     7021 tgttataatt attgatgtga aattaataaa tcgaaattcc ttttttggat ttaacaatac
     7081 tgagtaagga aaaagtgaat tacttttgca aggaggtgtt atttggatgg tgctagcagg
     7141 tattttgatt gtagtacaag gctttttgac tggtccttta gcaaaaaaaa ttggtgagaa
     7201 gcgactaata ctgataggta tgttttgcaa agccttagta gcgtttgcca tggtattgac
     7261 aactgggttt gttagtgtgt tggtggtttt tggcttattt gcggtatctt ctgctattac
     7321 agtacccaca ttaaacgcaa ccctttcaaa aactgacaat cagcataaag gaacactgat
     7381 gggatttgcc agtaccgccg gaaatcttag caaagttatt gctcctttat taactgggta
     7441 tctgtatgaa agcaatattg aattaccata tataaccacg ggagggatag ccttaatcgg
     7501 agtggttatc tgctatatct ggataaaaaa tcaaaagaac aaatgatgga agaggacgtt
     7561 tatatggagg ttatacacag taactaatct ggtatagaaa ttcaattgag gcttttctgc
     7621 tttattaaag tcttgctact acttaaccct ataatatcct taattcatta tgacaaattc
     7681 caattcaaca gttttgatac acataaggag gtaacaatac tatggaaaat tatcaatgaa
     7741 gtgctaattc tttatcagtg aagttatcca taagtttttc tacctgtaat cgtctggtgc
     7801 tttttgccag atcattggta ggtaagaaaa attcatcaac agacacatga agtaatgata
     7861 caaggtcact tgtatgcttg gatgttgccc tttattttca atattagtta agtaacgtgg
     7921 gtcaatattt gtaattcgat tttagggaaa tatttaaaga aatattttcg ttttatttct
     7981 ttaaatcttc aagaatattg atagtttttt gaaagtaggt gtgtattatg aagaatgaga
     8041 aaaaaattac agtcgcattg ctcattgttt atttattgat tttatcatgg attattttat
     8101 ttaagctaca attttcattt tctgcgttag accacataag acaaataaac ttaattcctt
     8161 ttggtggttc agttattgca aatggaaaaa ttgacttcga tgaaattata aacaatgcca
     8221 ttgtttttat ccctgtaggt gcatatttca gtttgctttt taaaaataaa tctgttctaa
     8281 aagcaatagg ttctgtttta ggaattagct ttgtatatga gataattcaa tttatatttg
     8341 ctatcggagc aagtgatatt acagacttaa ttagcaacac attagggggt ataattggaa
     8401 ttgcattagt atatgtatta tctattgttc taaaggataa aacacataag atattaaatc
     8461 gaatagcaat agtatgtact gtattagtag ttgccttctt atttatgctt cttgctgtaa
     8521 ataatatatt ttaatgccat gttaattaca tgttgttttg gaaaagactt taatctgtca
     8581 aagtttgctt ttgaaattga acatgaaatt acaccaatgg aacaaatgaa agctataaat
     8641 ttttcattgg agaaagatga ctgtacaagt aaagtattaa tatacaatcc agacaaatgg
     8701 aaatatgtag aatactattt ctataaagat agacctatta aaaaaactat tggaaaaatt
     8761 tattcaattt tgcatttgtc acaataaaat atgttttgaa atattgaata cttaaatatg
     8821 caaaaaaaca aatgtaactg gctatggggc tatatctact aaaaagctat gtgataggtt
     8881 gggattaaat ttaaacatca cttctagaag gcatgaatac acaaggctga caaactatac
     8941 agttatgatt aaacgatacc aacgggattg aaaatttcgt gaagaacttt gagaagtcct
     9001 attacacatg agcaacagca acgaagctat tgaagcaagg acgcaggcaa tctagcggtt
     9061 atctgacaaa ggagttgtag gacttgaatg tagatactat cagaacggat ataccgacca
     9121 gctctgcggt tacagatgtg attgtgtaga atatcgagca gtcgggaacg gacactttat
     9181 ctgctaccta cgaagtagat cagcagataa aagagagaga acagacaaca gcggtcaagg
     9241 caacctatac cgtaaaagtc catgtggacg ctgacgggga tatggtaatc gttcagaacc
     9301 ttactcttgc accagcaatc gaaaaatcag actatgagcc aaagatatca gaaacagacg
     9361 caagcgtgga cgctgatacc gttaatgacg ctaccgcatt tctggaaaca ttctttgagg
     9421 tgtacccgac agccacagaa aaagaacttg cctattatgt atcggaaaat gtgattgaac
     9481 ctatcggcag ggactacctt tattctgaat tggtaaaccc agtctttaca aaggacgggg
     9541 acaatgtgaa agtcaaggta gctgtgaaat tccttgataa tcagacaaag gcaacgcagg
     9601 tgtcgcagta tgatcttgta ctccaaaagg atagcaactg gaagatttta ggataacatg
     9661 gaaatgggct tgtatcataa ttatagtgtg caagcccttt tcatattgaa gtatcaataa
     9721 tcaataatta taattaggta catgaatgga gggataaaat gttcaaaatt ggtgagtttt
     9781 caaaattgac acaagtttct actcgtatgt tgcgttatta tgatgaaacg ggtttattaa
     9841 aacctgctaa aattgattca ttaactggat atcgtatgta ttcagcagaa caaatctcaa
     9901 ctttaaatag aattatatat ttacgtgata gtggcttcaa tgtcgctgaa attgcattag
     9961 ccctcaattt cagtaataat gccattatag aacagcttga tagaaagtct attgaaatta
    10021 tggataatat acaaaaagaa caagaaaaac taaagaaaat tgagattgct aaaaaagaag
    10081 tgttacatgg caaaagtgaa ttgtatttta acataaccat taaagagata ccgagttatt
    10141 gcgttttatc tttaagaaaa gaaataccaa attattatta tgagggcgaa ctctggaaag
    10201 aactctctgc ttttgctaaa gaaaataaaa taaatatttc tgatgaaaca ttctcaatat
    10261 atcacaatga agaatataaa gaaaaagatg ttgatgtaga attatgtgcc attgtaaata
    10321 aaataggaaa agctacagga gattttaagt ttcgttttac cgaaccagtt tctatgatgg
    10381 cttgtactat ggtatatgga gatttttcta atatttctta tgcttatcgt tcatttgcaa
    10441 aatggcttca agaaaacagt caatataaga tgaaaggttc aagccgtcaa atagttcatc
    10501 gtggaccgtg gaacgagaaa aatccagaga actatctaac agaaatccaa attcctttag
    10561 aaaatctctt gtaaaaaatg ataatctgcc ttgactttaa catggtgtta aggtttatcg
    10621 tttatttatc aagaagaaaa cggaggaaaa tataatgaca acttataaag taaattcaat
    10681 aggtactatc tgcaacaatg aaaaaggtgc tttcattcaa ctagaagcac catatattct
    10741 agcattaagg gcattagacg gattcagtca tataaatgtt ttatggtggt tcagtgattt
    10801 tgataatacg aattttagaa gtgtattgga aacagaacag ccttataaaa atgctccaga
    10861 gaaaatgggg atttttgcaa caaggtcgcc agtacgccca aatccgattg ctttatcagc
    10921 agtagaaatt atcgatattg attatatgag tgggctgata caaattgctt atattgacgc
    10981 taatgacaaa acgcctatct tggatattaa accatacacg ccgagtttgg atagagtgga
    11041 aaatccgcaa gttccagatt ggtgtagtca ttggcctcac agcttagaag aatctgcttc
    11101 ttttgcatgg gaaagcgaat tcaattttta aggagtgatg aaccatgagt ttgcaggact
    11161 acatttttga aaaaccagta attgaaacag aacatcttat tttaagaaca atgaataaaa
    11221 atgacataga tgatttgaaa gaatggatta gtgatatttc tttatacgag tattggggga
    11281 aacgtccctc aaaaagcgaa aaaaatccag aattactctt tgataagtca aaaaaatcta
    11341 ctaaaagttt tcattggggt attgtccata aacaagatag caaagtgatt ggtgaaatat
    11401 ggatatatct tattgaaaat aatagaatgg caaaagtggc tttcagactt tctaagcatt
    11461 atcaaggtaa tggactaatg agtgaagtgt tgaaaaacgt aattgaattt tgtttttcaa
    11521 aaacagaatt acaaaaaata tggacagatg tgcatatttt aaatcatgct tcttacaaga
    11581 cattggagaa agtcggtttt caaagggaag gacatattcg aggggggaaa atggtaaata
    11641 cctattgcga ctactaccta tatggaattt taaaatctga ttatatcact aggtggtgct
    11701 atggctaata ttgaagcgac gattggcgaa gcaatgaaca atacagttcc aaaggtctat
    11761 gatacgaaaa gcattaaaag atatgtgatg aatttattag atatggctca ttaaaaagta
    11821 ccaaagaaat aaaagttacc atatagacaa aattggtttt ttatatcttt tgatttctta
    11881 taagataatg gcattgtgcg acaagctatg caaaaaattt tcatgtatac tttgattata
    11941 atttatacaa ggaggtactg atatgtacgc atatgaaaat attgaaatgt cattaaacta
    12001 tattgaacaa catttaagtg agaaaatcga aacagagaaa ctggcggaaa ttgcctgtct
    12061 ttctacattt tattaccaac gcttgtttaa aaggttagtt aagaaatcag ttcaagagta
    12121 tatcaaactt cgccgtttag caatggtaat agcagaattg aataattcag aggaaagaat
    12181 attagacata gctttaaaat atggattttc agaccatgct aacttcacaa gagcatttaa
    12241 agaagtttac catattactc ccgatgaata cagaaagaat gttcctatgt taaatacatt
    12301 tgacaagcca gaactttctt cacgttatat catgattgat gaaaatgtcc cattgattgt
    12361 aggaaatatt gttttggaaa ttcaaagaaa aacattaaga acaaaggaaa catattttgg
    12421 ttttgaaaaa acagtaaata tttctgaaca aattcctgct ggagaaagta caggaattga
    12481 tgttccaggt cagctatggc gcgaattcca tgcaaagaaa ctgcaaaccg aaaatgccat
    12541 ttttaacaat gatattgaac ttggtgtatc tcacttagca agtccagata aaggaacatt
    12601 cctttacttt gcaggaggta tggtttcatc aacagatgta tgtcctaaaa atatggtaca
    12661 acatacatta ccagcaggtg agtatattgt atgcaaaatt gaggcagaat cttttgaaga
    12721 acttgttact actgctttaa atcaagcaaa taagtatctg tttgaaactt ggcttccaaa
    12781 tcataatttg gttacaaagc cattcatggc agaaaaatat tacaaaggaa atgcagagat
    12841 taattatatg gaaatatgga taattcctgt gtaaacagta aatgctaaat aggaattatc
    12901 tgacgtaata aaaaggaaat agaaagctta taaaggaggg catagtccgt tgtgataaac
    12961 ataaaagata taaattatgt acctaatatt agtgaaataa gcgtttatat tgaaaactct
    13021 gcatttgatg agttgtatca gcatatgaac aatgaatata aagcagtttg caaaatcgaa
    13081 tacagtaaag atgtctgggc acgaggttgg aacgtaaagt ttaaaaaatc tgggaaatca
    13141 ttgtgtgttg tctatccgaa gaagaactac tttactgtat tggtggttgt aggtaataaa
    13201 gagaaagaag atgttgaaaa cttgttacct aggctttctg aatatgtaca ggaaatttat
    13261 cgtaacacta gagagggaaa tggacaaagg tggcttatga tagacttaaa atctaaaaat
    13321 aatgtatatc aagatgtatt aaggcttatt gaaattcgtt atgccataaa atgtgtaaaa
    13381 ccgtaataaa aatatttttc acatacgtaa tctcaaggta ttatcgttga gacaagtaac
    13441 ctatataatt atgggaaatg agatttttag atacttaaga aagtacttta gggggcttgc
    13501 gttatggaaa gatttaagta caattccaca caatcaaata caatctaata aaactttact
    13561 aaaaattgaa tgcaatacag ctttaaaggc tcgtacaaac tatatgtttt gtgcgggctt
    13621 ttttcattta ataggaattt ggattcgtca gagagtatat tataaaaata aaacgataag
    13681 ggtaccttaa aaataaggta cctcaggctg tagacaaagt cccggactct cgtctgggct
    13741 tttcttatat ttgagttatc cacaattcaa ctttttaaga gttatcttat taagaaaaaa
    13801 gtgtatgttt tccaccctat agttttactt ctgtgatatt tgcagatgcc actcaagtag
    13861 aaagtggtgg tacggcagaa gatgtcatgc agagttcaga aagtctggga ctaccgccta
    13921 attccttaga cacggaaagc tcaatcaagc aggggtgtaa gtattttgcg tccctgcttt
    13981 cttcctgcaa aaatcaaggt atcgacgatt tgaatgtagc gatacaatct tacaactatg
    14041 gcgggggtta tgtgggctat gtggcaggaa aagggaaaaa gcacactttt aatcttgcag
    14101 agagcttcgc ccgtgagaaa tcgggtggaa agaaagtaac ctacgccaac ccgatagccg
    14161 ttgcaaagaa cgggggctgg cggtatggct atggaaatat gttttatgtg gaattagtca
    14221 atcagtattt aactgttccg caggtgtcgg gggaactggc acaaaaggta atgaatgaag
    14281 cgttgaagta tcaagggtgg aaatatgtgt ttgggggaag caatcccaac acttcctttg
    14341 attgtagcgg acttgtgcaa tggtgctatg gaaaagctgg tatctactta ccgagaacgg
    14401 cacaaacaca gtatgacgca acccagcata tccctttatc gcaggcaaaa gctggggagt
    14461 tgttcttttt ccattcaacc tataacgctg gttcgtatgt aac
//
LOCUS       DAMJWU010000094        14148 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMJWU010000094 DAMJWU010000000
VERSION     DAMJWU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.098"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            461..1354
                     /locus_tag="PC460_002912"
     CDS             461..1354
                     /locus_tag="PC460_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF2601783.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1354..2748
                     /gene="gltA"
                     /locus_tag="PC460_002913"
     CDS             1354..2748
                     /gene="gltA"
                     /locus_tag="PC460_002913"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF2601784.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3114..5333
                     /locus_tag="PC460_002914"
     CDS             3114..5333
                     /locus_tag="PC460_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2601785.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5480..6226
                     /locus_tag="PC460_002915"
     CDS             5480..6226
                     /locus_tag="PC460_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601786.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6226..8106
                     /locus_tag="PC460_002916"
     CDS             6226..8106
                     /locus_tag="PC460_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601787.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKNYTRKVWF
                     FD"
     gene            8225..9616
                     /locus_tag="PC460_002917"
     CDS             8225..9616
                     /locus_tag="PC460_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2601788.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9723..10763
                     /locus_tag="PC460_002918"
     CDS             9723..10763
                     /locus_tag="PC460_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2601789.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11252..11905
                     /locus_tag="PC460_002919"
     CDS             11252..11905
                     /locus_tag="PC460_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2601790.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            11981..12190
                     /locus_tag="PC460_002920"
     CDS             11981..12190
                     /locus_tag="PC460_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601791.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12330..12896)
                     /locus_tag="PC460_002921"
     CDS             complement(12330..12896)
                     /locus_tag="PC460_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF2601792.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13057..13710
                     /locus_tag="PC460_002922"
     CDS             13057..13710
                     /locus_tag="PC460_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF2601793.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13868..14044)
                     /locus_tag="PC460_002923"
     CDS             complement(13868..14044)
                     /locus_tag="PC460_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601794.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5542 a         1399 c         2377 g         4830 t
ORIGIN      
        1 tatataataa aataagagta tattttatac aatatgaaag ctggtattgc aatagattaa
       61 agccttatac acgtttagtt tttaaagaaa ttagtgttat tatgaaaacc taagttaatt
      121 tattaaataa ataattgaat aaaaaaattt tattatgtat ttataaaatt tttgttacaa
      181 atttggcata atatagtata ctttaagtgt gtattaggtt aaatctataa caaattagac
      241 gtatgatata gacttagtgt ttattggcat ttacaagaaa tttgttaata tcaactattt
      301 gtagtttggg cagttataat tattataggg gggatgtcca atacaaaaca atcaaccaag
      361 aactaatatt atattataaa gagttatagt tttgtgtgtt tatgttttta attaaattta
      421 aagtgggaaa aatatatttg ttagaatgga gaatagcgag atgagtaata aaatagttag
      481 taagagacaa ttaacagata gtatttattt aatggaaata gaggcaccta gagttgcaaa
      541 atcttctcaa ccaggtcaat ttataataat taaaaatgat gaaaaaggag agaggattcc
      601 tcttacaata gctgactatg atagagaaaa aggaacagta actattgtat tccaaacagt
      661 tggtgcttca actaaaaagt tagcaatgtt tgaagaaaac gattttgtaa tggattttgt
      721 agggccatta ggacaagcaa gtgaatttat acatgaagat atagaagaat taagaaataa
      781 aaagatatta tttgtagctg gtggagttgg ttctgctcca gtttatccac aagttaaatg
      841 gtttaaagag catggattag atgtagatgt aataattggt gcaagaacta aagaattaat
      901 tatattagaa gatgatatga aaaaagtagc taaaaatgta tatgtatcaa cagatgatgg
      961 tacatatgga tttaatggaa gagttacaga cctattaaaa gatttagttg ataatcaagg
     1021 taaaaaatac gaccaagcaa tagtaattgg accaatgata atgatgaaat ttatgtgcca
     1081 attaactaaa gaactaaata tacctactat agtaagttta aatacaataa tgatagatgg
     1141 aacaggtatg tgtggtggtt gtagagttag tgttggaaat gaaactaagt ttgcatgtgt
     1201 agatggtcct gaatttgatg gtcatttagt tgattttgat caagcaatga gaagacagtc
     1261 tatgtacaag actcaagagg gaagagctat gctaaaactt gaagaaggcg atagtcatca
     1321 tcatagtaat tgtggatgtg gaggtaacaa ataatggatg ctaaaaaagt aaaagtacca
     1381 gttagagaac aagaacctgc tgtgagagca acaaattttg atgaagtatg cttagggtat
     1441 aataaagaag aagctatggc agaagctaat agatgtttgg cttgtaaaaa accaaaatgt
     1501 gtgggtggat gtccagtagg aatcgacata ccagggttta taacaaaaat taaagaagat
     1561 gatatagaag gtgctgctaa agtaatagct aagagcagtt cattaccagc tgtttgtggt
     1621 agagtatgcc cacaagaaag tcaatgtgaa ggtgtatgta tacttggaat aaaatcagat
     1681 gctgtatcaa taggtaaatt agaaagattt gtagcagatt ggtcaaaaga aaatgacata
     1741 aatttatctg atacagagcc taagaaaaat caaaaagtag cagtaatagg aagtggtcca
     1801 gcaggtctag cttgtgctgg tgatttagct aaaaaaggct atgatgtaac aatctttgaa
     1861 gcaatgcatg agccaggtgg agttttaact tacggaatac ctgagtttag acttccaaaa
     1921 caagctgttg ttcaacctga aatagacaat ataagaaaat taggtgtaaa aatagaaact
     1981 aatgtaattg ttggtaaaac aattacagta gatgaactta tagaagatga aggatttgaa
     2041 gctatattca taggttcagg agctggtctt ccaatgttta tgaatatacc aggggaaaat
     2101 gcaaatggag tattttctgc aaatgagttt ttaactagag taaacttaat gaaggcttat
     2161 agagatgatt atgatacacc tattagttct ggtaaaaaag ttgctgtagt aggtggtgga
     2221 aatgttgcta tggacgctgc tagaactgca ttaagattag gttcagaatc atatatagtt
     2281 tatagaagaa gtgaaaaaga gcttccagca agagctgaag aagttcatca tgcaaaagaa
     2341 gaaggcatta tatttaatac attaacaaat cctaaggaaa ttttagtaga tgaaaatgga
     2401 tatgttaaag gtatggtttg tataagaatg gaattaggag agccagatga ttctggaaga
     2461 agaagaccta tagaaataga aggttctgaa tttgtacttg atgtagacac agttataatg
     2521 tcacttggaa caagcccaaa tccactaata tcttctacaa caaagagtct tgatataaat
     2581 aagaagagat gcttaataac agatgaaaat ggtcagactt ctaaagaggg tgtatttgca
     2641 ggtggagatg ctgttactgg agcagcaaca gtaatatctg caatgggtgc tggtaaaaca
     2701 gcagcagcat caatagatga atatttaaaa gcaaaagtta atgcttaatt tgtaaattaa
     2761 agagagggtg tctcaaaatg aactttttag ttcatgaggc actctttttg tacaaaatca
     2821 aatatgtttt aatcatttca acaatgaaaa tttcattcct gtagaaagtc taacttttag
     2881 gtcttagtac aatctctatt ttgatgtagc cactttttta gatggtttaa taatttatca
     2941 gttattattg taatatttga tactaagtaa cataactttg tctaactatg ataaaatagt
     3001 tatacaataa aggtcttgta aggagtgaat aatgtgtggc taataaaatc agatttgtca
     3061 aatctaacat taaataatag aaaaataaat tgtgtaatta atggtatttt aatatgaaaa
     3121 aaagagaaaa agtatttgat gggctagttt ttaagcatat attaatctta ttaatactat
     3181 tagttttgat tgatttaaca agtaccatca tatatgcaag taactctatg gaaattactt
     3241 ctagaaatat gattgaggca tcaaaaagag aacttgaaaa ctatttagaa gtaaacatat
     3301 ctttattaaa agctcttagt caagatgata gattttctaa tggtgaaaca tcactaattg
     3361 aaaagggaaa attattaagt ccatatcaaa aggaatataa tctttttatg attggtataa
     3421 cagatacaaa aggaaatgct tcaagtacat atagggaaaa tgttaactca ataaaagata
     3481 agcctagttt tgagaaagca attaaaacaa agcaagtagt agtttcagat attgaagtca
     3541 gtaatgtaac aggtgacaaa gtatttatca tatatgtacc aattgtaaaa aataatgaga
     3601 tgattgggac catttttgct tctttttatt ttcaagatgt aaataattta ataagtagaa
     3661 gcaattttga tgattctatt aaatttctaa tgatagataa agattatact attatttctc
     3721 atccaaacaa aaaatatgta aatgataaaa gtaagatgct tgatttagaa gggaatatta
     3781 taggaaccac taaaagtgaa atcttaaaaa atataaataa aaaatgtcaa ggtgattttt
     3841 tatcttggga taattggaga ttatataatg taaaatatac gaacataaag tggactaatt
     3901 ggactttagt atcaaaatgt aatatattta agaactttaa aaatctaata gttaatttta
     3961 tgataaagtt gtatttttat atagtaatat ttatgatatt gtggaaatta agtaatgcta
     4021 aattaataga acaacttaaa aaattggctt attatgattc tttaagtgga ataaaaaata
     4081 aagaaaagtt tagaaaagat tctatgtata ttttaaagaa ttattatcaa gataattttt
     4141 acttagtaca attggatgtc aataaattta aatatataaa tgagatgttt ggatatgctg
     4201 aagggaataa gatattaatt catatatcac aggttttaaa taataataca aataaatatg
     4261 aaatttgtgc taggatggat aatgaccatt ttatattgtt gattgcttgt aacactgaag
     4321 atgaactttt aaatagatta tctaaaataa ataaagaaat ttgtaactta aatactacta
     4381 actcatctaa gtataaaatt gttatgtcaa gtggaatata taagattact aaaaaggatg
     4441 atattaaaaa gattgacttg ttaattgata gagcaaatat tgcagcaaaa agtaaaaagg
     4501 aaaaatatga acattcatat tcatttttta atgaagatac aagaaatcga ttgtacaagg
     4561 aaaaacgatt ggaagataat atgaataaag ctttagaaaa aggtgaattt atagtatatt
     4621 atcaacctaa gtatagttta gatgatgtaa atgaaattga aggagcagaa gccttgataa
     4681 gatggaatag tccagaattt gggtttattt cacctataga ttttgtacct ttatttgaaa
     4741 agaatggttt tattgtaaat attgatatgt tcgtgtttga agaagtatgc aaaacattaa
     4801 acaagtggat aaataaaggg tatactcctg taccaatctc tgtaaatatg tctagagttc
     4861 atttatatag ggataatttt atagaaaata taactgattt gataagtaaa tacaatatat
     4921 ctcctgagtt tattgagctt gaactaacag aaagtgttgt gtttgataat ttaaatatat
     4981 tgattgatat aatgaaaaag attaaagaga taggattcct tatatcaatg gatgattttg
     5041 gttcgggata ttcatcttta aatctgttaa aagacctttc ttttgatatt ttaaaattag
     5101 atagaggatt tttaatcgaa acaacagata caaaacgtgg aaaaattatt atttctaaga
     5161 ttgtggaaat ggcaaaagcg attgatatta aagtaatctg tgagggcgta gaaacttatg
     5221 aacaagtaga gttcttgaaa gagataggtt gtgataaggt tcaaggatac ttatttgcta
     5281 aacctatggt attagatgaa tttgaaaaac acttaaactt taaatttgat taatcattaa
     5341 taattattag actataagat accctaacaa cttacttaca aatatgtaag ttaattgtta
     5401 gggtattttt atggttaaag tagtatattc acgatattat ggtattgtag taaaaataaa
     5461 aaattatgga gaatataaaa tgaacatatt gagtattaga aatatatcaa aaacatattg
     5521 tggaaatatt ccatttaaag cattagataa ggttagtcta aatatagaaa agggagaatt
     5581 tgtgtctgtt atgggaccat ctggaagtgg taaatctact cttttaaata taatatctac
     5641 agttgataga cagagtgaag gtgaggttgt attagatgga tatgatgtaa gtaaattgaa
     5701 aggagaaaaa cttgctgaat ttagaaggaa acaacttggt tttgtatttc aggattttaa
     5761 tttgattgac actttaactg ttggcgaaaa tattatgttg ccacttacac tagagggggc
     5821 aagtatcaaa gatatgaata tacaaactaa aagtatatct aaatttttag gaatagataa
     5881 aattttagat agaaaaacct atgaaatttc aggaggtcaa gctcaaaggt gtgctattgc
     5941 tagagctatt ataaataaac ctgccatact tcttgcagat gagccaactg gaaatcttga
     6001 ttcaaaatct actgatgatg ttttgaaatt atttactagg ataaacaaag aacaaaaggt
     6061 aacaacactt atggtaactc atgaagcata tagtgcaagt tattcagata gagtaatttt
     6121 tataaaagat ggttgtatat atacagaaat aaaaaaatct gaaagcagta attctttcta
     6181 ttctgatata ctagctgttt tgtctcaaat aggaggtgta agatagtgga ttttagaaag
     6241 tttgcttata ataatatagt tagaaatttc agggcatatt tagcatattt tttaagttct
     6301 gttttttcta taatgatatt ttttgttttt gcagtgtcta tgttccatcc aattataaca
     6361 aaatctggaa tgcagagtgg ttctactgca tttatggctc ttatgacttc agaattaata
     6421 atattcggtt ttagtctact ttttatattg tactctttag gaaatttctt aaaatataga
     6481 ttgaaagatt ttggagtatt gatgataatt ggaatgagta acagacaact gaaaaaattg
     6541 atattaattg aaaatttgat aataggattt ttagcagtga tgttaggaat attacttggt
     6601 ttatcaattt caaaattgtt tttattgtat ttaagcaaac tgttttatat gaatttaact
     6661 gaattttatt ttccagtaaa agcaatgcta ttgactattg tatcatttat gattttattt
     6721 ttgataacag gaccaatgag cttaaaatta ttgagtaaaa agagcatttt agagctttta
     6781 gtaggtacaa aaaagcctaa aaaagagata aaatcttcaa agctatttgg aataattggt
     6841 gtaatatgtc ttatattagg atatgctatg atactatttg gcaataagtt aaaaataaat
     6901 ggaggtatgt atacaacagc actattaatt actttgggga tatttttatt tttttctcaa
     6961 tttactattt tgatattaaa gtatttaaaa aataaagaaa aattttataa gaacaaagta
     7021 aatatgttac ttataagtaa tttgatatat aaattaaagg ataatacacg attgttattt
     7081 ttaataacaa tacttttatc ggctacttta gtagcattta caacaacttc aacacttgta
     7141 agttctcaag gagaagcttc taaaaataat ttcccaatgg tttactcata tttttcagag
     7201 cataataatg aaaaagaata tgaagaacta caacaaatta gagaagcaat caaaggatat
     7261 gattataagg agttaagttt tcctgtactt aaatataata gggaagtctt actaagtgta
     7321 aagaattata acaaattatc aaaggaattg ggattagata aaattagttc aaagttagaa
     7381 aaatatgaag ggattataat tcctagtagc aattcgcaag aacatataaa ttctttaaaa
     7441 caattaaaaa attatagtat aaaaaattta aatatagaag taaaaaaatc aaaaaataaa
     7501 ataatttttc ctacaggact gtttagtaaa gttgtggtta taagtgatga cttatacaat
     7561 gaagtaaaag gtgaattttc aaaaattaat tttcatggat ttgattataa aaactggcaa
     7621 agcagtgcaa agataactga aaatttaaaa gaaaaacatt ttaatttgga tagagaatat
     7681 aacaggtcat attttttaac attaccagat atgtatttag tagagttaca acaaagtaaa
     7741 attcttcaat ttatgggtgt ttttatagga gtgatattat ttatagcagt ttttagtttt
     7801 ttatacttca gattatatac agacgagata gaagataatg taaagtacaa aaaactatca
     7861 aaaataggtc tatcttttaa aaatatgaat aagattgtta gtatagagat aggtacattg
     7921 ttttttatac cgtattttat agcaattata aatagcataa tttctttact gtttttaaat
     7981 caagtattaa ataatagttt taagttagaa aatttgggta tattactact gatatcatct
     8041 atatattcac tttattttta cttattaaaa aaaaattata ctagaaaagt ttggtttttt
     8101 gattaaagtg gtatataata gatatataaa ttaatattta aaaagtagtt aatattaggg
     8161 gagggcattt gcacctccct tgtaatatgt ggaaaattga atattagaag aaggaggaga
     8221 cttgatggaa aatcaacaat tattaggaac agaacgaatt agtaaattac tactaaaata
     8281 ttctatacca gctataatag gtatgttggt aaatagttta tataatgtag tagatagaat
     8341 ttttattgga aatataccag gggttggacc acttgcaata acaggtcttg gtgtaacaat
     8401 gccaataatg acaataatac ttgcatttgg gatgttgata ggtataggaa ctactactac
     8461 catatcaata aaacttgggc aaggtaaagt agaagaggca agaaaactta taggaaatgc
     8521 aatgacgtta tctgtaataa caggaattat cataatgata cttggaatat tatttgcaaa
     8581 taaaatactt acattgtttg gagcaagtga aaatacttta atatatgcaa aatcatatat
     8641 caatattata ttattaggta cagtagtaaa tcttctttcc ttttctttaa accactcaat
     8701 aagagctgat ggtagtccta aaatttcagc aggaataatg atagtaggat gtttaactaa
     8761 tatagtttta gactggatat taatatttgg attcaatttg ggaatacaag gagctgcaat
     8821 tgctacagta acttctcagg ctcttacagc tattttaact atagggtatt atataagtgg
     8881 aaaatcaaac ttacgattca gtaaatctaa tctaaaatta gataaaaagc taataaaagc
     8941 agtgtttgca ataggtatgt cgccatttgc tatgcaactt gcagcaagtt tagttcaagt
     9001 aatatcaaat attgcactta aaactcatgg tggagattta gctatagggg ctatggctac
     9061 tatttcttct attgcaatgg tatttttgat gcctatcttt ggaataaatc aaggagcgca
     9121 acctattata ggtttcaatt atggagctga aaaatatgat agggttaaaa aagcatattt
     9181 aggttcatta gtagtagcta ctataatttt atgtatgggt atggtagtag ttatgctttt
     9241 cccagaagct attataggta tatttaataa agaccctgaa cttatgaata tatctgttaa
     9301 tggattaaga atatacttac ttatgttgcc aattgtaggc ttatcagtta caggaacaaa
     9361 ctttatacaa tctataggaa aagcaaaaat ggctatgtta ttgagccttt taagacaagt
     9421 aattttattg ataccagcag tattaattct tcctacattc ttaggtttac agggtgtatg
     9481 gacagctcaa ccagtgtctg attttatagc tacagtaatt acaggtatag ttgtatttag
     9541 agaattaaaa agatatactc ctaaaactga aaaattaaat gaaaatgaaa gactaaacga
     9601 gataactact gagtaaaaat ataaattcag ataattgagc aagaaattgc aaaaaatacg
     9661 attatatgat aaaataaaac cgctgtcaag gataagagaa attctaatta tggagtgatt
     9721 ttatggaatt tacaaagaaa agcaaattaa aaaatatgta tgtaaacatg aatgtagcta
     9781 gaatgttgtc taagataaat gagtataagg gtagacaact gctttacaaa aaacaaccaa
     9841 aagaaatttt agaaaactta gaaaaaaaat ctttagtaga ttgttctgaa tctacataca
     9901 ttggaaatca aagagatagt agtaatttta atctagaaaa gttgataagt aatgaagtta
     9961 cacctagaag tagagaggaa ttgagtattg ttgaatacag agatgtggta aaaactatta
    10021 acagtgctta tgagagtatt cccatcagtt cacaaacaat tcttgagctt catggatact
    10081 tatataaatt ttcatctact agaggaggaa gttataagtc agataatgat tttattgaac
    10141 acaatttaaa acctagcgaa tttataagta ctgattcaag tgtcaataaa gttgaaaaag
    10201 ctgtagaaga aatatgtgaa gcttataata cattgataga ggaagatgaa attgatatat
    10261 taatattaat ttctgcattt gtgttagatt tcatattgat acatccattc aaagaaggta
    10321 atataaaaat ggcgagagta cttatcctat tgttactaaa taaaaatggg tatgaagttg
    10381 gaagatatat aagtttagga aagatatttg atgatagttc atatgaatat tatagtaatt
    10441 taaattattt gaaagcctca ataggtagtg aaaaagcaga tatgaatgca tggattgagt
    10501 attttttaga gacgatatta actgcatatg agaagctgga tgatagttta aatatatctg
    10561 ataagaaaag acaaacaaaa actagtagaa tagagaaaat aataaattct acccttggat
    10621 attttacaaa agaagatata agagatttat gtcctgacat tccagaacct actataaata
    10681 gagtttttaa taatctaaga aaacaagata agatagaagt tgttgcaaga ggaagaagtg
    10741 caaaatggaa gaaaaaatat taaaaaatgt ttagataact aaaatggtat agtgttttac
    10801 actataccat ttttaatgtc attagcatga gcaataattt aacaacttaa gcaatatcaa
    10861 ttttatttat tgattatttt tgaactgaaa aatggcgtta tatggtatac ctcagtggta
    10921 attaattttg gatgagaaga tataatatta cagaatatat taggagtata tgtagaaaaa
    10981 atataataaa agtgtgaggc ttagtaatta acaaattatg tggcttcaca cttttttata
    11041 aaataaagat acttagcaga ataatatttt agttgatatg tttataagat ggtacttgtt
    11101 tgggtaaaag tataaatagt aaaaaagtag aagtaatgtg tataaaaaca taagttacat
    11161 tctgtaataa tagttatttt ataattcaac aaaagaatag aatgggtata tttgtgaaat
    11221 aataatataa aataatctta ggagtgagct tatgaagaaa aaattattat atataaatgt
    11281 aaattcaaaa cctgaagact tatcttcaag taaaactgta gcaagaaaat ttataaataa
    11341 atttatggaa aaaaataaag attttgaggt tgaagagata gacctttata aagaacatat
    11401 accaagactt gagtatcagt actttgaaaa aagaaatagc atagttagtg aagaaaatgc
    11461 taaaaattta gacgataagg atagaaaaga gcttacaaag attagaaatc tttgtgacca
    11521 atttgtgaat gcaagtgttt atgtaatagc agcacctatg tggagtttat cttttccagc
    11581 accacttaaa gagtatatag attgtataat tcaagatgga aaaactatat cttttgaagg
    11641 caatgataaa ccacaaggaa ttttaaatga tattgataga agtatggtat atatacaatc
    11701 ttctggtgga cacataccat gggtgttgaa gccagttatg aataaagggt taaattatgt
    11761 ggaaagtatt atgaaattta taggaattaa aaaatttgat gaattgttag tagatggaac
    11821 tggaactagt gaagaagaaa gacaagctgc aatagaaaaa gcatctgaaa aaatagatgg
    11881 tataatagat gggatgaaat tttaattata atattttatt aaaaatataa aggaaaaacc
    11941 tcttttaatg tagaaactta tattgaagga ggcgagaatc atgcgagaag aaaaaagtaa
    12001 tgaaaagtat gattgttatt ggtgtaatca agagaataat ttctgtgtag aaataaaaga
    12061 taatatagtc atgatagatg atggcactgg tacgttaaaa caagccgttt tcatagggta
    12121 taaacaaatc caaattaatt taaactgttc acattgtcaa aacttaaata gaataaaatt
    12181 aaatttgtag aattttaaaa tggaaattat tgaaggaatt aaaaatagaa agtgttaata
    12241 tttagactga aaattatatt ttatacaata aaagtttatt taaataacat tgtattttaa
    12301 tatttatttt cagtcttact ttattaaaat tattttttgg ttatgtctgg aaaatatctg
    12361 tctactaatt tttctgaaat attttttaat tggtcagcca ttgaagtcag acttaataag
    12421 gctatcaata aatcatcttt attttctgga tttatactaa tattttcaaa attagactct
    12481 ataaagtccg gtatattctt tctttgattt tcttttatta acatgaattt ttcgtaacat
    12541 gaaactaaat ctttttcact gaaattatcc ttttcataca caccatgtaa gattctttta
    12601 atttcttgta ttgtaagtgt atttttcata gaataaataa ttatcatctg taaaatttgt
    12661 tctttagtat acttttttcc cttaactggt ttgattaatc cctctttgct gtaattattt
    12721 atcatggttt tagtaagaat tttatcactt tcaaatcttt tattagcaga aagcttattg
    12781 tcaattagag aaataatttg atccatatat aagtcaatag atggaatatc gtttgaagtt
    12841 atatctgcat tatttagtgt ttctagtatt atagtattaa gttcattatt catcatatgt
    12901 tacctccata atatatataa tacactaaat tagaaagtaa aacaatatta aggcttgtaa
    12961 aaaaaacata aagtggtata attaaatata taatatagta atcaaaacta tatttatgaa
    13021 tctgattttt aaaatacata attgtaggag gacgaaatgt atcaattttt tttaaagggt
    13081 agagacccaa taagcagttt aactcatttt ataggtgctt gtttatctct tttagcaact
    13141 ataattttag tttttcaatc tgttacatta caggaaactt ctctcttgat gatagtatct
    13201 gtatctgtat ttggactttc actaattgct ttatatagtg caagttcgta ttatcatttt
    13261 ttaaagggga caccagaaca agaattgttt tttagaaaag tagaccatgc aatgatttac
    13321 gtattgattg ctggctctta tacaccaata tgtctgaatt ttatggaaaa aaaagaaggc
    13381 ataatatttg ttacagcaat atggatagtt gcatttattg gcataataat aaaaatattt
    13441 tggatggatg cacctagatg gttatctacc agtatatatt tacttatggg gtgggcaatt
    13501 gtttttgata taaatgcatt caattcaatt cctaaggatt gccttagact attaattatg
    13561 gaaggtgtat cttactctat aggtgcgatt atttatataa ttaaaaagcc taatataagt
    13621 cctgagtttg gatttcatga aatatttcat atttttatta tgataggttc tttatttcac
    13681 tttttagcag ttttattgta tgttttatag ttttaaaaga tttgattaat tgatactaaa
    13741 attctacaat gaataaaatg gaactgcctt atatagatta tatcttagac aataagacag
    13801 ttccttatta taaatattaa attctactta taataaaatt ttgataaaaa tagtccattc
    13861 aaaatgccta ttccataatt cctttaatgt acttttcatt tacgacatat agtttgaatt
    13921 tatgtactat aattatatcc tctaaattct ctgaaacact aacaattctg acacatttgt
    13981 ttctaatttc tacaggtaaa tctaaatcat ttgttaaatt cacatagtca tcatatttat
    14041 acataaaatc atccctttac attaagatat acttattata ctatatttta ataagttttt
    14101 ttaaattatt acaatttttc ccgaaatttc attttttata cataaaat
//
LOCUS       DAMJWU010000095        14074 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMJWU010000095 DAMJWU010000000
VERSION     DAMJWU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.099"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            163..1314
                     /locus_tag="PC460_002924"
     CDS             163..1314
                     /locus_tag="PC460_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2601795.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1432..2070
                     /locus_tag="PC460_002925"
     CDS             1432..2070
                     /locus_tag="PC460_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2601796.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2071..3093
                     /locus_tag="PC460_002926"
     CDS             2071..3093
                     /locus_tag="PC460_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDF2601797.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3083..3703
                     /locus_tag="PC460_002927"
     CDS             3083..3703
                     /locus_tag="PC460_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601798.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3827..4513)
                     /locus_tag="PC460_002928"
     CDS             complement(3827..4513)
                     /locus_tag="PC460_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF2601799.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4527..5072)
                     /locus_tag="PC460_002929"
     CDS             complement(4527..5072)
                     /locus_tag="PC460_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601800.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5300..6547
                     /locus_tag="PC460_002930"
     CDS             5300..6547
                     /locus_tag="PC460_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2601801.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6634..8628
                     /locus_tag="PC460_002931"
     CDS             6634..8628
                     /locus_tag="PC460_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2601802.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8628..9950
                     /locus_tag="PC460_002932"
     CDS             8628..9950
                     /locus_tag="PC460_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2601803.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10229..11515
                     /locus_tag="PC460_002933"
     CDS             10229..11515
                     /locus_tag="PC460_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2601804.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11601..12908
                     /gene="grdB"
                     /locus_tag="PC460_002934"
     CDS             11601..12908
                     /gene="grdB"
                     /locus_tag="PC460_002934"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12648..12650,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2601805.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13186..13917
                     /locus_tag="PC460_002935"
     CDS             13186..13917
                     /locus_tag="PC460_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2601806.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT         5637 a         1401 c         2449 g         4587 t
ORIGIN      
        1 tataagttta actataaatt aggtttatat tttataaaaa tttgctaaaa aaaacaatta
       61 aaaattgtaa agaaaaaaga tataattaga actgtagtaa taaatgtttt attatagata
      121 atagagtttt aagtatctta aataatattt ggaggaatat gaatggaatt aagaccaaga
      181 agtgcaaaat tagaagaatt tgataaagta atagaattaa taaactatgt atttagaata
      241 tcaagaaatc ataaaccaac tatgatggaa gaatttcctc ttttactaag taaaaataat
      301 atagaaaata tgattataat atcagaagat gataaagtgg tttctgatgt aaactatcta
      361 attcaggatg tatctattca aggaaacaga ttgaaggttg cagctatagg tggagtatgt
      421 actcatccag attatgaaaa gagaggctat tcatctaaga tattggataa agtagaagaa
      481 aaaatgtttt atgatggtgt agatattgta attatatcag gaacaagaag tctatacagt
      541 agaagaaatt gtagtttggt aaagagtttt tataaatata caataaaacc tgaagatgta
      601 aaaatagcat atgaaatagt tgaatttgat gaaactaatt ttgaaaaaga taatgattta
      661 gataaaatga ttgaactata taatcaaaat agtactagat ttataagaac gagagatgag
      721 tttcaaaaac tattgcatgc agcaactatt gcctggggac caattggtta caagaaggta
      781 tttataaagg aaaataataa tataatagga tatttgataa taagaacaat aaaaaaagaa
      841 gattctactg taggagaagt tgctgaaata gggttaaact ctgtaaatgt tgaaaatata
      901 ttgaaatatg ttgcaaataa atttggatta gaatctttaa attataaagt acatgtgaaa
      961 aatcttaaag accagttgaa atgtaatggg acaaaatctt tggattatca acaaggaact
     1021 atgaaaataa taaattttac taagttgtgt gatagtttaa gaagttattt tagtcaatat
     1081 gtagattttg aattactaaa atacatggaa tttaaacaag ttgaaaataa atatattatt
     1141 aaatacaaag aagaagaact tgtaatagaa aatttggata agcttaataa actgtttttt
     1201 gaaaaaaatg aggaacagta caatgaattt aaacatctaa aaaatatata tgaatttgct
     1261 acaaaggcat ttcctgtaga ttttccatgg actgcaaatt taaattatca atagttttag
     1321 atgatattaa ataaaattga ttgataccta gagttagtcc ttgttaaata agagaaatta
     1381 ttggatattg aaaaagcaat tttgtaagta attagaaagg atgagtatta tatggacata
     1441 cttgatgata tagcagaatg tatattaaat atgggtgtag ataatataga gaatttagta
     1501 aaaattgcta tagataaaaa tattgatgtg gaagatattt atgaatatgg tttaaacaaa
     1561 ggtatgatag gagctttaga taagtttgag aataaggaat actatctttc agaagttata
     1621 gtatgcacag atgcattaaa taaaggtatt aatcttttga agggaactgg aaaagtaaag
     1681 aaaaaatcaa agggagtaat tctgatgtct gtagtagaag gagatactca tgagatagga
     1741 aaaaatattg taaaagtaat ggtagaagcc actggatata aggttattga cttaggtgta
     1801 aatagaaaaa gtgaagatat aatagaagaa gctataaaaa acaatgtaga tataataggc
     1861 ttatcttcaa tgatgacaac tacaatggaa aatatgaaat cggttataga cgaacttaat
     1921 atgattgaga tagataaaag accaaaagta ataattggtg gaggacctgt aagtatggag
     1981 tttgcagaag aaataggagc agatggatac agttcaaatg ctccaaaagc agttaaatta
     2041 ataaataaac ttataggagg tgaagtttaa atgttttcag atatgacttt acttcagtat
     2101 ataaatagtg aagatagagg tttttttctg cctgatatgg gtacaaatgg attgttctta
     2161 ataaataaaa aggcatatga agtatatgat agtgtagatt tgcaattaga attagcaaag
     2221 actatggaca agtattttga atcagatttt atatactcat tctgtgatgg gattacattt
     2281 tgtgaaactc tgggtttaga tacattaaga ccagattatg attttccaag tgtattgaca
     2341 catactataa ctgacagaga taaattaaga aaattagatg ttccagaccc ttatacgagt
     2401 ggacgtatgc cactcaatat agagagctta tcagttatat caaaaagtat taaaaaacca
     2461 ctatatgaat cgatacaagg tccatttaca cttgcaggtc aattagctgg agctactcaa
     2521 cttttaagat gtataataac tgataaaaaa tttgtagaag aattattgga atttacaact
     2581 gaattggtaa gaagatatgc tgtagctgca aataaagcag gagcaaaata tatttcaata
     2641 gcagagccaa catcagtaac cctaagtaaa gatagatttg atgaatttat agtaaaaaat
     2701 ttaaataaaa tctacaatga acttgattgt tggaaaggta tgcatatatg tggagacacc
     2761 agagaattat tagataatat gctaagttgt aatatagatg cagtaagttt agaccaaatt
     2821 ttagactatg aagaaattgc tccaatgatt cctagagata tagttttgat tggtaattta
     2881 gacccaataa aacttcttgg acgttctaag ccagataaga ttagaagaga gacactaaaa
     2941 ttgttaaaga agatgagaag atatgataac tttttatgtg attttggctg taactgctta
     3001 aataccactc cagtagaaaa tttacaagca gctataaagg caggaagaat tagttataaa
     3061 gaattagata ggattgatat tgatgaatta taatagtaat aaaaataaat atcttttaaa
     3121 ggatataaat ttagatgata tttttataga taagaatatt gtgctaaagt ttttggggta
     3181 tggaaataga aaagctccag atacaattag taaaatcgtt gaaagagaaa taagaaatat
     3241 aaatgatatt ttagatatca agatatatat aagtgaagtt gatattaata aaacacctaa
     3301 agaatgtaaa aaagcatttg ctgtactata tacaataggg gataaaattg attttaagat
     3361 gaatgactat atggaaaatt gcaatatgat ggcagggctg gcattggata aggtaggtat
     3421 agtatgttta gattgtatta atgagaaaat aaaaatgtat ttaaaaggaa agtatgatag
     3481 tctcaaaata tcgcatgaaa tatatccagg agataaagat tttgaagtag agagacaaaa
     3541 ggatatttat aactatgtaa aaaataaata taatagtata gaaatagaaa ttaatgatta
     3601 tcatcaacta tcccccataa aaagtgttgc aatgcttatt ttaatggggg atgaagaaaa
     3661 tttagaatca agatgtagta agtgtcctag aaaatgcttt tagttaattt tcaagactaa
     3721 gatttgtttt gtgtataaat tgcaataatt tttttggtca ttttatgtat aaactaaaaa
     3781 aattattgca aagtaatatt ctatttaatt tgaacagact tacaaactat agtagtttct
     3841 ttaattcaat aagtgtattt atttcataag ttggtatatg attagtataa tttatactgt
     3901 tattggaatt taaccaacaa gtatctattc ctgaatttat tccacctaag atatcagagg
     3961 ttaagctatc cccaattatt aaggcagaag atttatcatg acaattaaac tttttgagag
     4021 cgtatttaaa tatttcagga tttggttttg atattttcaa ctcttcagat acaaccattc
     4081 catcaatata ttcttttatt tttgaatttt caagtctttt cttttgaact gcagaaattc
     4141 catttgttat tataacaata gtatactttt ttttaagata ttgtaatata tcgattgcac
     4201 ctggaattaa aaatgtacat tcacctaaaa aatttaagta cattttgcta agagtttcag
     4261 aatcaacatc caaagatatt ttatttgcaa aaagttcaaa tctaagtaat ttaagttcat
     4321 tcaaagttat aatatttttt tcaaggtcga gccaaagctt atcacttata tttctatatg
     4381 actctatata attctcaaaa ttaaattcta aatcaaactc agatagaagt tttttaaaag
     4441 catgtgattc acttttctta aaatcaaata aagtatcatc tgcatcaaaa aatataaatt
     4501 tatatgtctt catggttttt actcctttac aagatgactg caaaaattac tttactttca
     4561 ctatttgagt cattttgcca tttatgtttt gttcctaaag gaacttttac actatctcct
     4621 ttggacaaag taaattcatc atcatccata aaaagtttaa cttctccctc caaaacatat
     4681 gcaatttcat ctccattatg agtaatccta tcaacacaag aagaagtgtt tggagctaaa
     4741 tccataattg caaattctaa attaccacta gagtctggaa ttaaaatctc atatataact
     4801 tctttgcttc caggtaaaat aacttttttt ctattatcat gtcttactat caagtttttt
     4861 ttatctacct cagatacaaa aaatgtaaat aaaggaacgt ttagagaaga ggctatagat
     4921 tttaatgaat ttacagatgg attagcaaga cctcgttcta tttggctcaa taatgatggt
     4981 gtaacatcag ctagttttgc taaatctcga atacttaagt tttgtttttt tcgaacttca
     5041 gctatttttt cccctaaatt taaatcattc atataaatat cctctctttt taatttaaaa
     5101 aatattaact taaaataatc aagtaaaatg attatgtagt atgaaattat tataacatat
     5161 agaattaggt tttgaaaaaa ctatgttcaa tataattaaa taaattaaaa tataattgac
     5221 aaagtggata cagaatactt aaaatatatt taaataaaat taagcaaaag atagttaatt
     5281 tatgatagga ggggatatta tgcaaataac gacttttgga gctatatttg gtttattaat
     5341 agcaattatt ttaattataa aaaaatttca agctgtatat agtttaatgc taggagcatt
     5401 tattggagga ttagttggag gagcaaatat aacacaaact gtagatttta tggcaaatgg
     5461 agctatgaat atatcaccat caattcttag agctttggca tcaggtgtat tagctggaag
     5521 tttaataaaa acaggggcag tagataaaat atcagaacag attgtaaaaa tatttggtga
     5581 aaagagggct ttattttcga ttgccatttc aactatggta cttgctggag taggcgtaaa
     5641 tttggatgta tctataataa ctgtagcacc tataggatta tatataggta gaaaattaaa
     5701 ttattctaaa ttgtcaatac tattagctat gttgggaggg ggtaaagcag gtaatataat
     5761 atcaccaaat ccaaatacta tagcagtagc agataatttt tctgtcaatc tctctagtgt
     5821 tatgatggca aatataattc cagcaattat tggagttgtt attacagtta ttttagcaag
     5881 tatattaata aataaaggaa ataaagtaca atcatatgaa attttagagc aaagagaaga
     5941 tttaccaagt ttatttaaat cattatgtgg tccaattata gctatatttc tattgttttt
     6001 aggaaatgta tctcctatag ttatagaccc aatgatagcc cttccaatag gaggaatcgt
     6061 aactctaatt gtcacaggaa acttgaacaa ttcaagagaa tatttagctt ttggattatc
     6121 taagatgcaa ggggtatgca tattattact tggaacaggg actatcgcag gaattataca
     6181 gatgtctgaa ttacaacaga gtacaatagg tgctttacaa tttttaaata tgccacaatt
     6241 tttattggca ccagtttcgg gtatacttat gtcattagca acagcatcat caacagctgg
     6301 tgctactata gcatcatcta cttttcatga tgctataata aatggtgggc tatcaccgat
     6361 ttcaggtgcc tcaatagtaa atgctgggtc tagtgtattc gagcaattac cacatggctc
     6421 actatttcat actagtgcag gtagtataaa catggatata ggtgaaagat ttaagttaat
     6481 tccatatgaa gcattgatag gaatagtaat gactataatt tcaacatcta ttcaattagt
     6541 tttataataa atataaatat ttaatgaaaa aagtaggatt ttgttattaa atattgaatt
     6601 tattatagta aaaaagaaga ttggagtttt aaagtgaata aaaaaaagat tgttattata
     6661 gggattattt attcattttt agtagtattt tcacttacaa atatgtatgt aaatatggag
     6721 tataatctaa atgtatttga atatattaag aagtcacttc catttacaga agaagaaaaa
     6781 aagtggttag aaaaacacaa gaatttgatt tacagctctg accaaagttc acctcctttg
     6841 agatataaag gtaaggaaga tggacagtat aagggaatta ttgttgattt aataaattct
     6901 ctgtcaattc aaattggtag agatttttat tttaaaccaa ataattggtg gaaagaatct
     6961 ttcgtaaatt caatagatga tagtataaag ttttttgact tgattccatc aaaagaaagg
     7021 gcaaataaat ttatatttac tgacccaata tacactttga gtgcaaatat attgaaagat
     7081 aaaaagtccc aagatataaa tagttacatg gatttaaagg ggaaaacagt agcaattcca
     7141 gaaggtgatt attctataaa ttttttaaag caaaaaattc aagatataaa cattttgttg
     7201 actccagata taaagactgg agtcaaccat ttaatgtctg gaaaagtaga cgcagttgtt
     7261 ggtgatgaac cagtattgcg atactatata aataattatg gactttcaaa taaatacagt
     7321 gttttaagta atcctatata tactaaaaaa gcagttcttg ctgtacctaa acaatatgaa
     7381 gagctggtta gtattttaaa taaaggcatt tttaaacttc agaaaaatgg agtgtataaa
     7441 gatttaaaga aaaaatggta ttctacgtat aatgaagttg atgatatatt gtatgagcga
     7501 ggaattgtgc ctagtattta tctttttata ggtataattt taatatcaat ttatgttttt
     7561 tatagctata catatttgtt aaagatagaa atcaaaagga aaacagaaca ggtaatagaa
     7621 aataaaaaaa ctttagaggc aacttttaat agtattacag atataataat gcttgtagat
     7681 gaaaataaca atatagtaga atcgaacaaa gtactatatg actttatggg tgaaatgagt
     7741 tataagatag cagatttgat tagcatgata aaaggtgtta ttgaaaatac cttctctgaa
     7801 aatactaata aaactagtga aatagaaata cacaataaaa ttttaaagat aaacactttt
     7861 cctgtagagt ataaaaaaaa taatactgag tatatagtag ttcttattaa agatataact
     7921 aatgataaaa tagtagaggc gaaattactt agagaaaaca aaatgatatc tataggacaa
     7981 ttagcttctg gggtagcaca tgagataaga aatcctttgg gtataataag aaataattgc
     8041 tatctcttaa aagataacgt tacaatggaa gaagtaaatg attgtgttaa atcaatagaa
     8101 agtaatgtag atagggcaag taatataata accaacttgt taaatttcgc aagaatatca
     8161 gatgataatt tagaacatat aaatataaga aattttattg aaaatatagt gaaacttcaa
     8221 tataaaatgt tacagttaaa gaatgttgaa ataaaaattg actgtgaaca taatttagta
     8281 tgctatataa atggagaatc gttaaaacat gtatttataa atttaatttc taattcaata
     8341 gatgcaatac atcaagatgg aaaaattatt atatactgtt atgaaaaaaa ttattgttta
     8401 tttatagatt ttaaagataa tggtgaagga ataaaagaag atgccctaaa ggacatattt
     8461 aatcccttct atacaacaaa accaattggt gagggaactg gtttaggttt atacattact
     8521 tataatgaga ttaaaaagaa taatggagat ataagtgttg aaagtaaatt gggggttgga
     8581 acttgttttc atataaaaat tccattaaat aaagaggtga ctatataatg aaaatactat
     8641 tagttgatga tgagctagaa tatggagtgg taatgaaaaa aattctacaa aagaagggct
     8701 atcttgtaga tatagcacta agtggtgaag aagctataaa tataattaaa aaagataaaa
     8761 attatgattt ggttttgtct gatgttatga tgaaaaatat ggatggagta cagctattag
     8821 atagaattaa atctattaat agagatatag aggttatatt agttacagga tatggaagta
     8881 ttgaaaatgc agttgatgct atgaaaagag gagctttatc ttattttata aaaagtaatc
     8941 ccattgaaaa tttattagaa gaagttgaaa aggtaaaggc aagtaaaata tctgtttctg
     9001 tacaaaaaaa taatctggaa tttactttgg aatctaaaaa tagagatttt aataatatca
     9061 taaaaatagc aaaaaaagct gcttgtaaag atgttaatat attaatcttg ggggaatctg
     9121 gtgtaggaaa agatatatta gctagatata tacatagcat aagtccaaga aagaatgaga
     9181 tatttgtacc tgtaaattgt tgttcttttt cagaaaatct attggagtct gagttgtttg
     9241 gtcatgaaaa aggttctttt acaggagcag tagatagtag aaaaggtaga tttgaacttt
     9301 cgaataaagg aactctattt ttggatgaaa taggagatat acctctaaat gttcaggtaa
     9361 aacttttgag gacacttgag gacaaatcta tagagaggat aggaagtaat aaatctatta
     9421 aagttgactt taggttaatt tgtgctatga ataaagaacc aaaagtggaa atatcaaatg
     9481 gaaatataag agaagatttt ttttatagaa taagtacaat aacaataact ataccaccgc
     9541 taagaaagag aagagaagac ttaactactc ttatagaatt tttcttaaat aagtaccaaa
     9601 ttgaacatga taaaaagata cactcgattg ataaggaagt aaaggatttt ttattaaatt
     9661 acaattatcc aggaaatata agagaattaa aaaatataat aaatagactt gtagttttaa
     9721 gtgaagaagg caatttatca aaagacaatt taaatttaat atcaaataat gtatacatag
     9781 atgataaaat aagtattaga cctttaagag aaataagaaa agagtttgaa tgtgagtata
     9841 ttgaaaaagt tttatctttg tgtggaaata atatttcaaa tacagctaaa aaattggaga
     9901 ttagtagaag acagttaacc aataaaatat cagagtataa tattaagtga gaaaaaaact
     9961 tctcattaag tgagaaaaaa atttctcaaa ctgtattaaa agataaacag aaaaaatctg
    10021 tttatctttt tttattataa ttttaaaaat taatatttac aataattatt gctaaattca
    10081 atattggtgt aaattaaaaa tgaaaatttc tatgacatta atacatagaa tatatatttt
    10141 aaataattgg catagaaaat gctttatata ttaataaacg taataaatac ttttaattgc
    10201 gattaaatat attaaggggg attttcatat gaagctagag ctagggaata tatttataaa
    10261 aaatgttgaa tttggagaaa agacagaagt aaaagatgga gtactatata ttagtagcga
    10321 agaaatagaa aagatagtat tagaagatga aaggattgta agtgtaaatg ttgaacttgc
    10381 aagacctggc gaatcaataa gaatagctcc agttaaagat gtaatagagc ctagagttaa
    10441 aattggagat gaaagcaaaa tatttcctgg aattataaat aaagtgaaga ctgtaggaag
    10501 tggaagaacc catgtacttt taggagcttg tgttgtaact tgtggaaata tagttggatt
    10561 tcaagaaggc gtaattgata tgagtggtcc tactgctaaa tatacacctt tttcaaaaac
    10621 aaataatata tgtattgtta taaaagcaaa ggatggtata gatactcatg attatgaaga
    10681 agcagctaga attgcaggat taaaaatagg tgcatatgta ggtgaggcag gaagagaagt
    10741 tgagccagat gaagtcattg tatatgagac aaagccatta ttaaaacaag taaaagaata
    10801 tccagacctg ccaacagtag catatgttca catgttacaa tcacaaggac ttttacatga
    10861 cacttattac tatggggtgg atgctaaaca gatagtgcct acatttatgt atccaactga
    10921 aataatggat ggagctataa tatctggaaa ctgtgtagca ccatgtgata aggttacaac
    10981 attccatcat ttaaataatc cagttataca tgacttatat aaaagacatg gaaaagattt
    11041 aaactttata ggagttattt tgactaatga gaacgtattc ttagtcgata aagagagaag
    11101 ctctgatatg gtagctaaat taatagaatt tttaggagtt gatggagttc tagtgacaga
    11161 agaaggttat gggaatccag atactgacct aatgatgaat tgtagaaaat gtagtgaagt
    11221 tggagcaaat gtagttttga taacggatga atttcctgga aaagatggca agagtcaatc
    11281 tatagcagac gctactaagg aagcagatgc agttgtttct tgtggtcaag gaaatctagt
    11341 ggttcatttc ccagcaatgg aaaaaattat aggaacttta gattatgttg aaatgatgat
    11401 aggtgggtat aaaggatgct taaatgaaga cggaagcatg gatgcagagc tacaaattat
    11461 aatagcatca actatagcaa atggctataa tcatttgaca gcaagatatt attaaataag
    11521 tatttgaata taaagttctg ttgatataat cattggtcta attgatgtaa caactataaa
    11581 gtgatttgga ggattaagag atgagtaaaa tcagagtggt tcattatata aacaatttct
    11641 ttgctggtat aggtggagag gaaaaagcag atattccgcc agaaaaaagg gctggtgtag
    11701 taggacctgg aatagcattt cagagtcaat ttaatgatga tgcagagata atctcaactg
    11761 taatttgcgg agatacgtat tttggtgaaa atattgagag tgcaactcaa gaacttttaa
    11821 aaatgataaa agaagaaagc cctgatttat ttattgcagg cccagcattt aatgcaggta
    11881 gatatggagt agcttgtggg actatttgta aggcagttga agaagagtta aatattcctg
    11941 taattacagg catgtataaa gaaaatccag gtgtagatat gtttaaatta gatttgcata
    12001 tagtgtctac aggaaattca gcagcaagtc ttagaaaggt agttccaata atgacaaaac
    12061 taggtcttaa attggttaga ggtgaggaaa taggacctcc tgaagaggaa ggatatataa
    12121 tgagaggaat aagaaaaaat ttcttccatg aacttagagg gtctgaaaga gcaattgata
    12181 tgttggttaa aaagatgaaa ggggaaagct ttgaaactga gtatccaatg cctgaatttg
    12241 atagagttac acctatgaaa cctgttaaag atttatctaa gataaaactt gccctagtaa
    12301 cttctggagg gatagttcca attgataatc cagacaaaat tgaatcttca agtgctacta
    12361 aatatggaat ttacgattta acagatatgg attctatgtc aaataaagaa tttacaacta
    12421 tacatggggg gtatgacaga gcatatgtcc ttgaaaatcc aaatttagtt gtaccactag
    12481 atgtagttag agagcttgaa aaagaaggtg taattggaga attagtagat tattttataa
    12541 ctactacagg aacaggaacg agtgttggca attctaagag atttggagaa gagttttcta
    12601 aaaaattgtt agaagatgat gtagatgctg taattttaac ttctacatga ggtacttgta
    12661 ctcgttgtgg agcaacgatg gtaaaagaga ttgagagagc tggtatagca gttgttcata
    12721 tatgtacagt agtaccaata tcacttacaa taggagctaa tagaattgtt ccagcagtag
    12781 ggataccata tccacttgga aatcctatgt taggagaaga ggaaagtaaa aaaattagaa
    12841 agaatatagt tttaaaagct ttaaatgcac taacagaaga tatagaagaa caaacagtat
    12901 ttgaataata aaaatacaac acaacactga aataaaaaaa gatatcaaaa aataattttt
    12961 taaaattatt ttacgatatc ttttttttat taggtaaatt cgaaatttgc attttttaaa
    13021 attaaattta taaaagtgat aaattagttt attataatat tgatacacaa attcaaatta
    13081 gatatactat gaaaagcgag attattacat tttggaacaa atattcaaca tattagatca
    13141 tattaattga acagaaaaaa ttaactttat ataataatta gaataatgat aagaaagaag
    13201 gtgtatatat tgatttcaat tgccatttgt gatgatgaat cctatatgct aaatcattta
    13261 aaggaaaata tagaattata catgaagcag aagaatttat tatataatat agagcttttt
    13321 aataaagtag aagatttact atgcaatcat gaaaagttta atattgtatt tttagatata
    13381 cagatggaag agattaacgg aatgcaggca gctagaaaat taaggtcata tggtaatgag
    13441 tgctttataa ttttcgttac agttttaaat gaatttgtat ttgatgcttt tgaagtagat
    13501 gctataaatt atctgataaa acctctatct aatgagaaac tatataaaac tttggataaa
    13561 atagttacaa aacttgatga taatgaaagt aattatatca caatacaaaa aggttattca
    13621 tattggaaat taaaacttgc tgatatttta tattgtgaag ttataaaaag aaagatttat
    13681 gttcatttaa agaaagagac tgtagattat tatgagacta ttgaaaattt agagagaagt
    13741 ttaccaacaa atttttttag gtgtcatcgt agttatataa tcaatttaca atatgtatat
    13801 ggatatgaaa atggtaatgc agttatggaa agtggagata gaatacctgt ttctagacta
    13861 cgtcaacaaa tattttcaaa agttatgctt gagttcatga aaaataggag gatataagat
    13921 gattaattta ttttcttccg atttattttt aaagtacagt tttttatttt cttttattgt
    13981 atatagaatt ttaatggtaa tagtgcaata ctattttcta atcagtttta caaatgttaa
    14041 ttcaaaattt tggtgtttta ttttatattc gata
//
LOCUS       DAMJWU010000096        13994 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMJWU010000096 DAMJWU010000000
VERSION     DAMJWU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.100"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            402..1052
                     /locus_tag="PC460_002936"
     CDS             402..1052
                     /locus_tag="PC460_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2601807.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1255..1926
                     /locus_tag="PC460_002937"
     CDS             1255..1926
                     /locus_tag="PC460_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2601808.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1923..3053
                     /locus_tag="PC460_002938"
     CDS             1923..3053
                     /locus_tag="PC460_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2601809.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3484..4329
                     /locus_tag="PC460_002939"
     CDS             3484..4329
                     /locus_tag="PC460_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2601810.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4329..5357
                     /locus_tag="PC460_002940"
     CDS             4329..5357
                     /locus_tag="PC460_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601811.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5360..6373
                     /locus_tag="PC460_002941"
     CDS             5360..6373
                     /locus_tag="PC460_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2601812.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(6890..8140)
                     /gene="cme"
                     /locus_tag="PC460_002942"
     CDS             complement(6890..8140)
                     /gene="cme"
                     /locus_tag="PC460_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF2601813.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8226..8972)
                     /locus_tag="PC460_002943"
     CDS             complement(8226..8972)
                     /locus_tag="PC460_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2601814.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9125..10147)
                     /locus_tag="PC460_002944"
     CDS             complement(9125..10147)
                     /locus_tag="PC460_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF2601815.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10147..12567)
                     /locus_tag="PC460_002945"
     CDS             complement(10147..12567)
                     /locus_tag="PC460_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2601816.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12653..13573)
                     /locus_tag="PC460_002946"
     CDS             complement(12653..13573)
                     /locus_tag="PC460_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2601817.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         4951 a         1812 c         2194 g         5037 t
ORIGIN      
        1 atttacatta ggagttttct atactataat tatttaaaac taaatataaa tgttggaatt
       61 atttaggact tgaaattgtc taaaaataaa tacaagtgat gaagaggtga tgtgatgaga
      121 ttagatatgg atagtatgtt atatgtacta atttatatga cattattggt atctatcatt
      181 ggaagtatat tttatttttg taaagacctc ttaaatcata aaattaagta tgttttaata
      241 agagacatat ttgtaaatag aattacatat tctgaagaac gactgcctaa acagtatata
      301 gtttttcaga aatatgatat ttggaggtat tgtagtttgt tttaatttga aatgaatcta
      361 ttataaattc taaagtttaa aaataaaaag gagagaataa aatggatttc atttcaagta
      421 tattgcaagg attattaagt atattattta attttacagg tgattttgga atttctattg
      481 tacttataac tttaatagtt aaattagtac ttatgccact atcattaaaa caaagatttt
      541 ctgtaaaaaa acagcaagaa ttagctgaaa aaatggaata tataaaagaa aagtataaga
      601 atgatactaa agaattggaa aagcaaatac aagtaagttc tgtagaaagt atgaagagta
      661 tgctgggatg tcttatagtt ttggttcaaa tgccagtagt atatgcttta tatcatgtat
      721 gtttagatat gacaaaggaa tttacaacaa ttataattcc atggattgct aatttaggta
      781 catttgataa tttgtttata ttaccttgta tatatacatt aattatgtta gcttcaaatt
      841 tagtgaatta tataccatat ttaaaaataa attctcaagt aaagtttaac aaacaaatgg
      901 ctgtggtgac taccataaca agtcttatac taactgttag aactccagta gccataggtc
      961 tgtactttat aacaagtgca atttattcat taattgaaga tatttgcttt agaatatatt
     1021 ttaatcgaaa aaatagaata gtgacaaaat aatataatta ttattagaaa tattttagcc
     1081 tgctaacata gaaaaagtta gtaggctatt tttatctatg atatcaattc cattaagaat
     1141 agtctaaaaa atactatcaa aatgctataa aaaatactat aaaaaaatag ttgaaaaata
     1201 aataaattaa tataaactaa aataaagcct acaaaaaaga ggtgataaat agaaatggct
     1261 aaaatactag ttgtagaaga tgaaaaaaga atgcaaaata ttattgtaga atatatgcaa
     1321 aagggaggat atacttgtat aactgctgat gatggagtcg aggcacttac aatattaaag
     1381 agcaacaata tagatttaat gatattggat attatgatgc cgtatttaga tggattttca
     1441 gtgtgtagag tttcacgtga aatgactaat atacctatta ttatattgac agctaaaggt
     1501 gaagaggagg ataaattaaa gggttatgaa tatggcgcag atgattatat aacaaaacca
     1561 tttagcccaa aagtactttt agcaaaagtt aatgctttgt tacgtagata cactactgat
     1621 attcccaaaa attcattgag tttaggaaag atttttatta tggtagcatc aagacaagtg
     1681 tatgtagaag ataaattgat tgatttaaca tataaagaat ttgaattatt gcgtttattt
     1741 atggaaaacc ctaatcaagt tttttccaga gagaaacttt taaattgtat atggggatat
     1801 gattttgaag gaaacacacg aactgtagat acacatataa aaaccttacg caaaaagctt
     1861 ggtagtgaag gacaccatat agttacatta attcgttcag gatataagtt tgaggttaag
     1921 gaatgaatag tttaagaaag attatagaaa aactgaaaaa tgtagatatt tcaaattgga
     1981 ttaaacaaaa ttttggacta tattcagtta gaaaaaaagt gtttttactt tctaaattat
     2041 caggagcgtt aattatatta ttttatctgg ttgttgaaga attttcgata aactctaaat
     2101 ttgaattttg gatttggttt ataatactgg ttatatttat tatgagtatt gatttcttat
     2161 tgggaaaatt tatttcagaa ccaatcacat ctataaataa atctgcaaaa agtatgtccc
     2221 aacttgattt ttctaatcca tgtactgtaa atactaatga tgaatttgga gaactttcta
     2281 ggagcctgaa tacaatgtct acaaatctac aacaggcatt atcagaccta gaaagtgcaa
     2341 atattcaact tgaaaaggat gtaaataagg aaagaatgct attggaacaa agaaaggagt
     2401 tggtagatac aatttcacat gaaatgaaga ctccacttgg cattatacgt gcgtatacag
     2461 aggggcttat agatgaagtt gatgaagaaa aaaagaaaaa ttatatgaat gttataatag
     2521 aagaaactga tagaatgaac aatatgattg tatcattatt agacttatct gcattggaag
     2581 caggagtttc taaacttaac ccagagcgtt ttgattttat agagtttgtt gaaactgtgg
     2641 caggtcgtct tttaattgat ataccagatg ccaattttta ttttacatat gatttgccag
     2701 agtatgaagt ttttgtagtg gcagataaaa tgagaatgga acaagtagta gagaatctag
     2761 ttattaatgc aaagaagcat gttgtatata atggtaattt agatttatcg attacatgtg
     2821 aaaatggctt gcttttattt gaaatttata atgatggtag gtttatagag caagatgaga
     2881 tttcaaagat ttggtctaag ttttatcgta gtgttcaatc tcagcgtaca ggaggttcag
     2941 gtctagggct tgcaattgtt tcacaaatac ttacaatgca agggttagaa tatggagtga
     3001 ataatcgtga tagaggagta gagttttatt ttatgattcc aattgatgaa taatttagta
     3061 ttaatttata aattataagt atctgatgta aaatattttt atttgtatac gtaagtattt
     3121 aaagagaata aaataaaaaa acttaatgaa aaagcttaat gaaaaagtct aataaaaagc
     3181 ttaatgaaaa gcttaataaa aagtcttgtt ttcctgatga aaacaagatt ttttattaag
     3241 ctttttatta tttcacttcg atttcacaag agagacactc tgatttcacc aaatagaaat
     3301 aaaataggtt aaataaatta aaaaatagaa gcgtaattaa ataaggttac aagggggagt
     3361 acttatgaaa tggtatacat ggttgactat tatattgata atattgcttt ctattccgct
     3421 taaaattaaa tttataaaat ggtgggatag acgacaaaaa aatgataaag gaggttatga
     3481 tagatgataa aagtaagaga gttaaaattt tcttatggaa aagataaaca gatattgcat
     3541 ggacttaatt ttgaagtaaa agaaggagaa atctttggat tccttggacc taatggttca
     3601 ggaaaatcca caacacagaa aatcttaaat ggtgttttaa aaggttacaa tggtcacgta
     3661 tccatttttg gtaaggaagt taaagcatac acagaatctt tatatcaaaa gattggtgtt
     3721 ttatttgaat ttccttattt atataccaat ttaagtgcaa ttgataattt agaatatttt
     3781 tcgtcttttt atcctaaaaa tcaaaggaga gacatatatg aactcttaga tttgttagag
     3841 tttaagaaag agtttataaa taagcctgta tcatcctact caaaaggtat gaagcaacgt
     3901 attagcatgg caagagcatt ggtaagtaat ccaaagttgt tatttttaga tgagcctact
     3961 agtggacttg acccatctgg agctgtacta tttcgtaaga ttattgaaga agaacgaaaa
     4021 aaaggaacaa ctatcttttt gactactcat aatatgctag atgcagattt aatgtgtaac
     4081 aatgtggctt ttattgcaga tggaaagatt atggtaatag ataaacccaa aaatttaaaa
     4141 atgaaaaata gcaataataa agttgaagtg gaatttgtat ataatggaaa tcgagatatt
     4201 gaaattgtag atatacaaga gttagaatct ggaataactt ttaaatatga tgaaatctta
     4261 agtgtacatt caaaagaacc tacactagaa gaagttttta ttaaatacac aggaaggatg
     4321 ttagtgtaat gtattctaaa ttttttacct tagtaaaaaa agatttttgg atgatgattt
     4381 atggtaaatt ttttgtgctg acacttggct ctctcatact ttattcgtgt tatattaatt
     4441 ttggttatgt aaagtttatg gatgaagata gttatcacaa taatgtatat ctatataatc
     4501 cagagaatat aagcatgagt gcatctccat taattaatca agtttcttct ataggtgaat
     4561 taaaagcaaa acttgatgaa gatacaagtg cagtttgtat tgatatgagt tccagtaagc
     4621 cgaaagtaat tttacatgaa agtattaaag gagttgacaa ttatagagct gactatgcat
     4681 tgtcattatt atcatcagaa aaagaccata atagcaaaat atcagacaac aatgcaataa
     4741 ttcaaattaa tgataaggat acatctgaga taaaacaaag aaaagacatg acttgtgaga
     4801 ttttattctt tgaaattgta gctattggat ttttagggat tgcctcttta ttatttaaag
     4861 aaaagcagat gggagtaatg cgtattactg gtattttacc tgtaaaaaga agtcttttta
     4921 tattatcaaa gatatttgct ttttttatat gtgacctatg ttttacagtt cttttagtag
     4981 tatttaatat tggattttta gaaggaataa aaatacttcc acaagtatta gttcaagcta
     5041 gtattttatc aattattatg tctttgattg gaataggatg ctcaatgtta ttgagagatt
     5101 ttaagcaatt ttctcttata tatcttgtgg ttgctgtatt cataactaca cctatatttc
     5161 tatctgcaaa tacatctttt gaaatagatt ggattaaata tcatcccttc tatcatgttt
     5221 atagtcagct taaaaatgcg tttttcatga atttttcaaa agaattagtt tattttgtag
     5281 tgtgttcttt gacaataata gttctatttt tacttgtaaa atatattttt aataaagaat
     5341 tggttaggga gggataatct tgataggatt acgttaccag ttaaagagta taaaaaaaga
     5401 taaaatgtgt ataatttcct tctttttacc agttgttatg gcgattttaa taaattttat
     5461 gggaacaatt gatatatctt cattaggaga gatacgtttt ggtatagtga aggatacact
     5521 tcctgtacaa acagagcagt ggttaaagaa gtatggtatg gtaagtgtat atgacacaaa
     5581 aatgcattta atacgtgaaa ttaaggatat taaaacggac acaattggag ttgttagaga
     5641 tggtaaagga ataaaaactg tgttttatgg aaatgagatt aatgctataa aaaatgttgg
     5701 aaagacattg ccagagatgt ttagactaaa gggtgattta aaagatgttg aggttacaat
     5761 attgcctaaa gatagtattt taaaagagtt tcaaaatgtt tttattgcta tgattttaat
     5821 tactgctatg tttatgggat gcacctttaa tgcaatgaat attatatcgg aaaaagaaga
     5881 tggaatatca tttataaatg aagttttgcc acagagtaga agtcagtata ttatacaaaa
     5941 agtatttttg ggtcttttat gtgggtgttt atcttcaatg ataactgtat ttatatgttt
     6001 ggatatttca atattagata tgataatgat gttgttactc gttgtatttt cagcatttat
     6061 ttcatcactt ataggtttat ttattggaaa gttgtcagaa ggtcttatgg taggaattgt
     6121 ttatattaaa atagtaatga ttttatttat aggtactcct ttgttaatat atcttctagg
     6181 ggttaatacc agtggtatag ggaatttatg ctatataatg ccttcagttg ctacatttaa
     6241 tggtattatg gagattatta atggtaatgt cattattatc aaagatattg taattttggc
     6301 tgtacattgt gtagtatggt ttatgatata tattatttta gataaaaaaa gaaagttatc
     6361 ttttagtaga taactcagta taattataat tgggacaatt taataggtga tttaatatac
     6421 tttgtatagt gtaatttatg tagtgcaatt tgtatacttt aatttatgta gtgtaatagt
     6481 tagttttatt ttataattat tcaatataat ttagtaagaa gttcagtaca attagagcta
     6541 cttagtggaa aacttaatag gtacttaaac tatataaata ctttattttc ctccatttgt
     6601 actataaatt taatttatga tataaatgga ggtattatta tatgatttga taaatttcat
     6661 cattataacc tacaagtaaa ggtattatta tataaagtta ataaagatag tatcaaaata
     6721 ttatttgcta taaagagagg gactcaaaat aaagataaac ctatttttca atgttgaaat
     6781 gataaaatat atttgatttc atataaaaaa ttttcttcat aaattaaaaa gtccatttta
     6841 agccccttca tttttaacca gataaatcga gttaaactat ttaaatatat tactcggatt
     6901 catttgactt agatattcct tgcttgtaaa attgttttga ataggggata gccaaagcaa
     6961 aaaataaaag tataatccct gctatgatta ttaaattatt aatagaaatt acatctgcca
     7021 ttggtccgaa aatcatcata ccaagaggaa taaaaccagt atatataata ataggtaagc
     7081 taagaactct acctagcatt tgtggtgcta catgctcttg caacatagtc atagctgatg
     7141 cttgaatgat tggaattgaa aaattcgtaa aaaacataaa tataaaaaat tgccaagctt
     7201 gtgttacaag acctagaata attgaaaaaa tagcatagga taatattgct agaaaaaagg
     7261 tcttatttct atttttaaaa ccaccaaaaa ctcctataat caatccacct aaaaccatac
     7321 ctaaaaatcc aatcatttca ataatagaca ggtatatata acccttgcca aatgttcgtt
     7381 caataagaag tcctataaga aatcctgatg gaacacaaag aagcatgaat gctccataag
     7441 taaaaagcac ttttctaatg aacttatctg aaaaacagta ctttatccct gataccatat
     7501 ctgcaaaaaa tgagactttt tcagtttgtt ttggtatatc atgtttagga attttgatga
     7561 atattaataa tacaatagca ataattgcag taaatacatc aattaaaaga acttgattta
     7621 ttttattacc aaatccaagt attgcagcag ctacaatagg aactataaat tgtactatag
     7681 actgtagact actatttata ccattaaaac gcattaaatg ttcttctggt acaagttgag
     7741 gaatcattgc gtttacagca ggtgtttgta caccacttcc tagtgaacgt ataacagata
     7801 ttactgatat ggctattaat tcacttgttg tgccatttga tgacatcata aatagaatta
     7861 gaactaatgt agatatagca attgttgaat ctgatacaat tatgagtacc tttcgattgt
     7921 agtagtctgc ccatacacca gcaaaaaaag aaattatcat ttgaggtata aaggagcata
     7981 cagttaaaac tgttacccat acacctgatg atgtctgaag tgtgacatac catatcattg
     8041 ccatttgtac aagtgatgag ccaaataagg aaatatattg acttgctaaa aaaagaatga
     8101 cttttttctt ccatgtagaa ttagtatttt tattttccat cctattcctc ctttgatttg
     8161 tgtgttgaat ttttcttgct cttaaggata acacacaaat caaagtctgg agttagagtg
     8221 gagggctagg aaaattgttt tttcataatt tgaagatgag atttcatggt ttttgcatta
     8281 tgttcagctt ctttcaatga agttatcagt ttgtcacaca ctgagataat ttcgtcatca
     8341 tcttttggag tgtcaaggac agttttaata ttagtattag gactagaaga taaagcattt
     8401 aacattcgta aaatagctga aagagaatag tttgcacaac gaagtgcgct tataatttta
     8461 agtcgtttta tatcttcatc agtatataca cgataaccat tttgttttct ttttactttt
     8521 aacaaaccat tcatttccca atttcttaaa gtatctattg taacgtttaa gtgttctgca
     8581 gtttcttttc tagttaaaaa gagtttattt gtttctggag tatttccatt taaaatctgt
     8641 gcaacaatag taagtgcttc ctcggcatta gatttttcta tttctaattg ttgcaagtat
     8701 tgatttacaa gactaatagc cttatcaaag tccctattcg ctgatgttct tacaatctca
     8761 agggcatctt ttcttaatcc cctttgtacc acttcaattt tcaaggctgt acgagcaaat
     8821 ctaaattgtt ctatatgaaa atcattaaag atacgataac cattggtatt acgcttaggt
     8881 ttaggtatta agtttagttc ttcatataaa cgtactgtat taggatgtac accaattaaa
     8941 tctgctacct ctgatgtcgt gtaagtgtcc attagtattc aactcctttt agatatatag
     9001 tattagtcat aataacacca aacaaaagtc tggtgttata atggagggtt tggctatcta
     9061 agagtaatta gtttatgaaa gagtaattag tttatgaaag agtaattagt ttatgaaaga
     9121 gtaattaatc tattatagaa gtaagtatat atttttcaat gaactcccat ctatcacgag
     9181 gtttaaacac tattttagat ggtatggcta ttactaagtc atattgtgga atacaacaaa
     9241 tagcatttcc tccagttccc attgctgaat accacaaaac accatctttt ttacatagcc
     9301 accacagata accgtattca ttactattca ttttaactga ttcttcaatc cacgatttag
     9361 atataatacg tttattattc caaataccat catttaaata cagaaaacca aatcgcaaca
     9421 tatctctagc actcaatgta agtccccagc cacctataga ataaccctgt ctatcttttg
     9481 cccaaccctt aagatttttt ccaaagaaat cttcaaaacc aaaatctttc atatcataat
     9541 ctggtataac tttcatacca ataggtttaa ataagtactc attagcaaat tcacgagcag
     9601 attttccagt agtttttgtt ataatagctg aaagtaggtg cgttccagct gaagaatatt
     9661 tgaaagtacc aatttcgcct ttttgaccaa gttgattcaa ggtatatttt atccaatctt
     9721 tttgtttgca aagtctttct aaagactcag tcatattttt aaaagggtat ggtgcagtca
     9781 ttgtaagtag gtgacgtata gttactgctt ttttacatat ttcagaagga ctacaagtat
     9841 attcaggaaa aaaatcaaca accttttggt caacatcttt tatatatcct ttatctattg
     9901 caatacctat taaggctgat ataatacttt ttgtaactga tgcaacatga aaagtatcat
     9961 cagcattata tccattatag tacttttcaa agacaatttt tccttttctt gcaataatta
    10021 taccatttat attgctatat tgtgagatta ttaaaggttc aagttgtgaa agcttttctg
    10081 aactcatacc aacacttgaa gggtctactt tttcaaaaat taagtctaag caattgtttt
    10141 tattcattaa agatacctcc ctgttataga ttttggattt tcttttagag tttttggaga
    10201 accttctgca attatataac ctcctttgtc accaccttct ggaccaagct caacaatcca
    10261 gtcacaaaca tttaatacat ctatatcatg ttcaacaaca attacagaat ttccattttc
    10321 aaccaattca tctaataatt gaatcaactt agaagtatca tatagactta gaccagtagt
    10381 tggctcatcc aatacataaa ggatattacc atttcgtttt ttaccaattt cttttgcaag
    10441 tttgattctt tgggattcac caccacttaa agtcggtgta ggttggccta attttatata
    10501 ccccatacct atttgttcta atacatttaa gtgtgaaaca atatttggaa tatctttgaa
    10561 gaaatgtaca gattcagaaa cactcatatt taaaatatca tgtatatttt tatttttata
    10621 tttaatagat aaagattcat cattaaatct ttttccctga cattcactgc atatatggtc
    10681 tatacttaga ttatttccca accagacctt ttcatatcca ctaccactac atttaggaca
    10741 agcccctttg gagttaaatg agaaatgacc agctgtaaat ccttgttcta acgattctgg
    10801 ctgacctgca aatagtgtac gtatcttatc ccatatacca atataagaag caggagttga
    10861 gttcatattt ttgcctatag gagattgaga tatctctgaa tatccagaaa tatgtttagt
    10921 accaatcagt ctatctgcaa tagtttcaac aactgcataa tcgtcttcct cacctatttc
    10981 actctctgaa gaatttattt gattattgat agtatggtta ttaaattggc ttctaagaag
    11041 aggaagtagg gtgtctgata taagtgaact cttgccacta cctgattttc cagatattcc
    11101 aaccatagca cccaaaggta atgatacagt tacattttta agattatttg tatttgcatt
    11161 ttgaatagtt atacaaggta ttttatcagt atgtgtaatg tttttattgc ttgttctatt
    11221 aggcattgga tattttccag atagatactg tccagtaagt gatttgtcgg attgaagtag
    11281 accttctaaa tctccttgat atactaattg accaccttca acaccagctt ttggaccaat
    11341 atcaataata tgttctgcca tttcaattgt gtttttatca tgctcaacta ctatgaccgt
    11401 attgcctaaa tctttaagtt tctttagtga ttttaagatg tctatttttt ctgattcatg
    11461 caatccagct gttggttcat caagcacata aattaatgag tccatctctg aatcgagatg
    11521 tgaatttaaa aatagtcttt gcatttcccc accactcaaa gttgacattt ctcggtataa
    11581 ggatagatga cctaatcgtg attctattaa atgttgagtt ttggataaaa tatcatttac
    11641 aagatttgta ccaaatgtgt ttaaattacc ttgatttatt aagagtttaa ggaaatcatc
    11701 aatttctaaa atcgtcattt ttccaagttc tccaatatgt tttccgttta aaagtactct
    11761 acgtgcttct tcaccaattt taaaaccatt gcaatctgaa caggcagtca tagtataaac
    11821 tccatttaaa tcttcgcccc ttttgtaacg attgtgaaat acctttgata aagaggaact
    11881 ttttttacca ttttcataat gaccatagag tacttcattt cttatttctt caggtagttg
    11941 taagaattgt atgtctaaat aatcattgaa ttttttagaa aatactctca aatatccagg
    12001 tgtaatcttt gctctatcta agacttgttt tagggtatca tgcttatcaa ggactaattt
    12061 ttcaatatta atctcaaaat atgcaccatg tccagagcac ttcatacaca ttccatctga
    12121 agagttgtat gagaaataac ttggtcttaa tcgttcttcc tcatatccac aattattgca
    12181 aattagatta tcgcttacag gagtatcaca catagtacac attatttgac cttccactga
    12241 atacaatagt gtaagcatgt tgagaatatt ggttcttgtg cctactgttg aacgaggatt
    12301 gctctggcga ataatatttt gttgaacagc tatagtggga gctaaacctt gaatattatc
    12361 aaacttatct tcactatcaa tacctgcaag tatacctaaa gattgcagat attgtttacg
    12421 cccttcctca aatacaatgt caaacataag gctagactta cctgagccac tgacacctgt
    12481 tgcaacaaca agcttatctt ttggaataga aacatctata tttttaagat tgtgtatctt
    12541 tgctccatta atttttattt ctttcattgg ttcacctcat tttatcaatt ttatcaaacc
    12601 gtaaaatagg gatatattaa tccttaatac aagaatagat taggtgaggt tattaaaaag
    12661 gttctatagg taaatatata tcgaccaagt gtctattttg agggtgattg tgtgggttgt
    12721 taagatatac ttcaaaaggg aaggaggcac gtagcttata attaccattt gatagccatt
    12781 cattatacat aaaatcccac gcatcactat attcagattt tgaaatatca aaatgtccca
    12841 caagatattt tccagaaggt attatcatat tgccaagatt gtcattctct ttgacaacaa
    12901 tgttatctgg aatggtcatg caaatactag tccttaattg atgctcctgt gtaaattgtg
    12961 gattatcatg ataaatggct aatatttgtg tgttttcaag tccaagtaga ttctgctcac
    13021 ttgcatatgt gaataaccgt tctaaaagtt ttgggaaagt actggttaga ttaccatatg
    13081 aaccagtata tctagtgtaa atagtattca tattatcaag ttctagaatt tcaatttcac
    13141 ctttaacctc tctagtattc ttacatttag gttttgatat gcttctattg tactgagaaa
    13201 ttttatatgg ttctttgcaa ttcttgctat aattgtttct atattttata gggcttattt
    13261 tatagtaatt tttaaatgct ctcgagaaaa ctgctgaatc agtaaagccg aagtagtgtg
    13321 caatatcagt aacagtcatg tcagttctat gaataagaaa agcagttgca ttttctagtt
    13381 ttagcctatt tacatattgt gccaaagttt ctttttctat tgctttgaag attctatgaa
    13441 aatgaaattt tgaaaaacct gctatattag aaagttcatc aagagaaaga ggttcatgta
    13501 aatgttcttc tatatagtct tgcactttgc aaatacgatt tagatattct tttttacttt
    13561 tagcatttgt catatatagc cctccttatt gtgtaaaatg tgtatcaatt aatttatagc
    13621 aatatagtaa taaaatttta tgtttttgta atttatgata aataagatag cgtttaattt
    13681 tttataattg ataaaagata taagatgcct tatatttgtc tttgcatata agtatagtct
    13741 tcgtaataat tagtatacca tagtgattta tttttaattg tgtatttata taaatacaca
    13801 acttatgata tggacgaatt attatgcaaa taagctgtaa ttaaataaat tgcaatataa
    13861 accagttgaa acataagtta gggaaatatt atcaaagtta ttacttagca taagagtata
    13921 gatggaataa aataagatta aaaaattaca tactaaattt aataaaattt ttatattaaa
    13981 aataatatta agtt
//
LOCUS       DAMJWU010000097        13526 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMJWU010000097 DAMJWU010000000
VERSION     DAMJWU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.101"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            322..1752
                     /locus_tag="PC460_002947"
     CDS             322..1752
                     /locus_tag="PC460_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2601818.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1855..2958
                     /locus_tag="PC460_002948"
     CDS             1855..2958
                     /locus_tag="PC460_002948"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2601819.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2973..3209
                     /locus_tag="PC460_002949"
     CDS             2973..3209
                     /locus_tag="PC460_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2601820.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3226..4275
                     /gene="vorB"
                     /locus_tag="PC460_002950"
     CDS             3226..4275
                     /gene="vorB"
                     /locus_tag="PC460_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2601821.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVIEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALTSKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4277..5017
                     /locus_tag="PC460_002951"
     CDS             4277..5017
                     /locus_tag="PC460_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2601822.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5020..5559
                     /locus_tag="PC460_002952"
     CDS             5020..5559
                     /locus_tag="PC460_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2601823.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5683..7782
                     /locus_tag="PC460_002953"
     CDS             5683..7782
                     /locus_tag="PC460_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF2601824.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIENRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8049..8564)
                     /locus_tag="PC460_002954"
     CDS             complement(8049..8564)
                     /locus_tag="PC460_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF2601825.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8656..9867)
                     /locus_tag="PC460_002955"
     CDS             complement(8656..9867)
                     /locus_tag="PC460_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2601826.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10543..11985
                     /locus_tag="PC460_002956"
     CDS             10543..11985
                     /locus_tag="PC460_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2601827.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            12027..12380
                     /locus_tag="PC460_002957"
     CDS             12027..12380
                     /locus_tag="PC460_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601828.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12571..13392
                     /locus_tag="PC460_002958"
     CDS             12571..13392
                     /locus_tag="PC460_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2601829.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         5165 a         1577 c         2429 g         4355 t
ORIGIN      
        1 aataaaatta taaaaaatta aaatcactag aaatatgata aaataattta aaatacgcat
       61 ttcaatgaca tttcaaaaca ataaaataat aaaaaacaaa cctaaattaa gtctaaatag
      121 ctttttagtt aattttagta gtaaaaaata aaacttaaaa agtgtttgac aattatgaat
      181 tttgagaata taattaaatt aaaattatac acatataaaa atatactcct gaatcaagaa
      241 tgagatattt aagattcaaa atatatatta ttatacgtga ataataaaaa agtaagaaaa
      301 ttttataagg gaggggtaga tgtgagtagt caagaaagca aaccaaaagg aaatcttata
      361 tcaaatttta gagatgatat gagagaccct aagaaaagag caatatacac atattcaagt
      421 tctataaact tagcgtacaa ttttataggt tactttgtat caactgttac aaatattttt
      481 ttaactgatg tggcaaaact tggtgtaatt atggcaggtg gtatgcaaat tatacaatct
      541 ttcataaaaa ttgctgtagc tcctataaca ggtacagtat ttgacaaaca accattcaaa
      601 aaaggaaagt attatccatg gcttagatat gcaccagcag cgttagcatt aacatatgga
      661 atatttttca tagttccatt aatgaaaata agtccagcat tacttttacc tttgatgaca
      721 gctttattat taatagcatc ttgtgctcag caagtagttt tcactataat ttatgctatt
      781 taccctgtag ttgctaagac acctagagac cgagttatag gctcaactac tacgaatata
      841 tttaaagaag ttggtaaatt cttagtaggt ttttcatatc cgttattatt agttttattt
      901 gcagatgtat taggtggaga aggaatgggt tatttaggta cctacttatt ctttgcagca
      961 ggatgccttt tagtattttg gttttcttca acagagataa taaaatctgg agcagaagaa
     1021 gaagttcttc aaaaagaaga gaagccaaag attaaagcaa gtgaaatggc aaaagcttta
     1081 ttcactaatg taccacttat gatggcattt tgtttagaat ttcttatatg tataagatct
     1141 ataggattag gacctttggc tccatattat tttaaatatg taatagaaga tgaacgtgga
     1201 ttagcagtat ttttatcaat catgcctttg gtaagtgtag cattcatgtt tttcgcacca
     1261 gtgtttataa aaatctgtag agagcaaaaa ctagcttcaa taatttcttt ttctatatgt
     1321 gcactatgcc acttattggt tgcatggact ccttggggga aaactacttt aggagtaact
     1381 atgttgctgg caattggtgg aggtttttca aatgtagtat caataataaa tcttaatttc
     1441 tttgccggtt cttgtgatta tgggcattgg aaatcaggaa aagacttacc tggcttaagt
     1501 atgtcactat atccagttgc aatacaagta ggagtattac ttgcaactac tataagaaca
     1561 gttttaatga actcaatggg atatcaagct gatatggtag tcacagaagc agttaaatca
     1621 ggttttataa atatgatttc ttattctatg gcaatacctt taataatagc tgttgttata
     1681 gcaatactat atccagtaag tgacaagaaa ttaaaccaaa taagagaaga gttaaatcaa
     1741 agaaatgctt agttaaaaga aagttaagta gatggttaat ttaagctatt aaagtaagta
     1801 gacgcaatag cgtttatata aatgaaaata catatacaga gggggctttt aaaaatgata
     1861 gaaaaattat tgagaaatac cgtaaaagaa ttacatcaat atgttccagg tgaaccaata
     1921 gaaaaagtta aggaaaaata tggtgtaaaa gaaataataa aattagcttc aaatgaaaat
     1981 cctctaggac catcaccaaa agcaatagaa gctatgattg aaatgttaaa acaaggtcaa
     2041 ctttatccag agccagaagc taacgaactt agaagaaaat tagctgaaaa gctagattta
     2101 aaaccagaaa attttattgt tgctaatggt gcagataacg ttatcactct aataggtgag
     2161 gcttttataa atagaggaga tgaagttata tattgtaacc caacatttcc atcatataga
     2221 actgcaacta taaaaaatga agggatacca gtagaagtac ctttaacaga agattataag
     2281 tatgatttac aaggtatatt agataagatt acagataaaa caaaattaat atgtgtatgt
     2341 aaccctaata atccaacagg aacaattgtg gatgataaag agttagaaga atttttaaag
     2401 aaagtccctg aaaatattat aactatattg gatgaagcat atatagaatt tttaacagtt
     2461 cctaattatg tggatggact taagtatgta agggaaaatt ataacgttat agtaacaaga
     2521 actttctcta aaatatatgg tcttgcaggg cttagagtag gatatggtat tgctaaagat
     2581 gagataataa gaactttatt tacagtaaaa gagcctttct ctgcaaatcg tgttgctata
     2641 agtggagcaa cagcagcact tgatgatgaa gagtttataa aagaaacata tgaattaaat
     2701 agagttggaa tggcttactt taaagaagag tttacaaaaa tgggatttga tgttgtagat
     2761 tcacaatcaa atttccttta tgtagatatg aaaacagata tacctaagtt atttgaagac
     2821 ttgaaaaaaa gagggtttgt aataagacca agtgctacac atgcacgtgt aagtattgga
     2881 actatggaag aaaataagaa atttgtagca gttttaaagg aaatattaaa tattaaagga
     2941 gagccaagta ttggataata gatagaaata ttatgaaacg gggtgtagat atgaacttaa
     3001 aaataaacgc agagagatgt aaaagttgtg aatactgcgt tatatcttgt aaaaaaggag
     3061 cattaaaaat atcaagtaag ataaacaaag aaggttatgc tcatgtagaa gttgatgaga
     3121 gtaaatgcat actttgtgga atttgctatc aggtgtgtcc agataatgtt tttgaaataa
     3181 ttaaagaaga agcatgtcaa gaggcttaga aggagttggg ataatatgaa aaagatgtgg
     3241 aaggggaacc acgccattgc agaagcagcc ctaagaggag gttgtgaatt ttatgcagga
     3301 tatccaatta caccacaaac agaggtaatt gaatttttat cacatagaat gtctgaactt
     3361 ggaagaactt ttatacagtc cgaaaatgag atggctgcta tatatatggt atatggagca
     3421 tatgcaagtg gaatgaggtc tatgacgagt tcttcaggac caggaatatc tttaaaacaa
     3481 gaagggattt cttacttatg tgcaaatcat tacccttgtg taatcgtaaa tgtacagaga
     3541 tgggggcctg gtcttggctc attggactca gcacagacag actatctaag agatactaga
     3601 gggggaggaa atggagatta tcatttgata gtttatgcac caaacagtat tcaagagact
     3661 gtagatttga tgtataactc atttgatgta gcggagaagt acagagtgcc tgtcgaaatt
     3721 ttgacagaag cagctttagg tcaaatgatg gaaccagtag aatttccaga gtttaagaag
     3781 agagaaggag atttaggctg gacttatgat ggaagtaata gagaccatgc aaaggttgca
     3841 gataaccaaa agccaacttt ttgtatggag aaacgtatga gaataactga aaatgaacaa
     3901 caatgggaag attatcaagt agaagatgca gaatatgtgt ttgtagcgtt cggtatacca
     3961 tcaagaacaa ctatgaatgc tgttagaaga cttagagaac aaggtgaaaa agttggaata
     4021 ataagaccaa taacagtatg gcctttccca tataaagcat ttgaaaaggt aagtaaagat
     4081 gtaaaaggat ttatctcagt agaatcaaca gacacaggtc aattagtaga agatgttgct
     4141 ttaacctcaa agaaagtatg taaagaaaat gtacctgtgt atggtttatt tagtggaaat
     4201 catataccaa aaacattgca agtaatggac acttatagca aaataaaatc tggcgaaatc
     4261 aaggaggtgt tctaatatgg ctacagaaaa attaacacct agcgttgttt caataccaaa
     4321 caagttttgc ccaggttgtg gtcatggaat agtaaatcgt ataatagcag aggtaataga
     4381 agaacatgga tatgaaaaaa atcatgtatt aacattagga gttggttgtg tctgcaatat
     4441 gaattttagc tggaatggtg ataagatgca aactgcacat ggtagagctt catctacagc
     4501 tataggagtt aaagtagcac ttccagatac attagtaatg acttaccaag gtgatggaga
     4561 tgcttatgtt ataggtcttt ctgaaacttt aaatacagca tatagaaatc ataatgtgac
     4621 tgtatttgta ataaacaata ataattttgc aatgactggt ggacaaatgt catggaccac
     4681 tatgccgggt caagtcacaa caacaagtgt aaatggaaga gatataaaga ctactggtag
     4741 tccactgaaa gttcctgaga tggtggcaaa cttctttgat gtagcatatg tagcaagagg
     4801 ttcagttcat agcccaaagg aaataattaa tctaaaaaaa tatataaaaa atgcaataga
     4861 agctcaatta aatggagagg gatattcctt agttgaaata ttagcacctt gtccaactaa
     4921 ctggggtgta tcactagaga aatctatcaa atggatggaa gaagaaatag tgccatatta
     4981 tgctcttggc gaatttaagc aaagggatgg tgaatagtta tggaaaaaga gctagtattt
     5041 gcagggtttg gtggtcaagg agttttaact cttggtctga ttgtagctga gtcagcactt
     5101 gaattgggga aacaagtaac ttggatgcct gcatatggtc caactatgag aggtggaaaa
     5161 gcctatagtg tagtaaagtt ttctgatgag agtataggtg ggcctgatat ggaagaaata
     5221 gatgttttag tagcaatgaa taaaccatct ttagattata taaatttagt taaagaaggt
     5281 ggaacagtag ttattaatac atctgcaata gatgagaatg taacattaag agaagatata
     5341 agtgtagtta aaataaattg tcaggaatta gctcaaaaag taaacaatcc aaaagcagca
     5401 aatatagtag tacttggagc attaattgct aaaacagggt tatttgaaaa agaattagct
     5461 ttaaaaacta tgtgtgattt ctttgaggaa aaaggaaaag gaaagtttaa tgttcaaaat
     5521 gaagcagcat tcatggaagg ctataataac gccatttgat aaattaccct tatcttaagt
     5581 gatttatctc aagatttaat aatctttagt gccaagagaa acttcatctt gagataaaat
     5641 tcacttaaaa tcacaactat aaacatatag gagcgtgaat ttatgagttt ggaaaaactt
     5701 attagaccaa aaagtattgc tatagtagga gtgactgata aacttggttt tggaagaagt
     5761 gcagcactta gtatagtaaa aagtaaggaa acagatagag tatattatgt taatcccaaa
     5821 agagaagaat tgtttgggag aaaatgttat aagacgatac aagaagttcc agaagtagta
     5881 gactgtgtag tagtatgtac acctagaaat gtagttccat ctgtattaaa agattctgga
     5941 gagttaggaa caaaggcagc agtggtatat gctagtggat ttgcagagga aggtacagag
     6001 gaaggtacag atttagaaaa tcaacttata gaaatatcaa atacatacga tatgaaaata
     6061 ttgggtccaa actgcatggg gttgctaaat tgtatagata aggttaatct atgggcagga
     6121 ggttctaaat gggatttaga tactaaaaaa cctggtatag gaattgtagg tcaaagtgga
     6181 tttataactg ctgaaattgt ttcaagtgat tattttaata tatcttatgg tttttcaaca
     6241 ggaaatggaa atatagttac actagaagat gcagtagatt tccttgtaga tgatgattat
     6301 gcatcagtaa tagcaattta ccttgaaggt ttaaaaaatc ctcaaaaatt tatagatgct
     6361 ttaaaaaggg cagcacaaaa aagaaagcca gtaataatat tgaaatcagg aaggtcagaa
     6421 aaaggtgcta tttctgctgc ttctcataca ggaaatttag ctggttcgag taaagctttt
     6481 gaaagtattt ttgaaaaata tggagtaatt tctgtggaaa atttagaaca gtttatgtgt
     6541 ttagcacaag cttttagtgt tttagatggt aatttacctg caaattctaa ttttgcagca
     6601 ataaacttct caggaggaga aaatactatt tgtgcagatt tagcagaaga aaatggtgta
     6661 gaacttgcag aaatatcttc tgaaactaaa gaagaaatga aaaaatatct tccaggattt
     6721 gcaacaccta aaaatccact agatgctaca acagcattat tccatgaaaa ggatatgata
     6781 gtaggtttat tacatacctt tgaaaaggat gatagtgttg gatttactat ggtaggtgca
     6841 aatataagag atgaagaaaa tgaaatgcat gaaacccttt gtaaagctgt aagtgaagct
     6901 agagaacaag gattgaaaaa acctgtgttt gcagttccta cacttgaagg aaatagatat
     6961 ttagattata ggactagatt agaagataat caagtgccta taatgtcatc agttggaact
     7021 acatttactt gttttaataa aatggctaag tttatagatt atgattattc aaaaagaact
     7081 ttagaattta aagctgttaa gaaaagagag tcaaataatg tagtagcatt atcagaatta
     7141 gatagtaaga ttgagatgaa aaaatatgga gtaccagttc caggacaagg taatgcaaaa
     7201 agtattgatg aattagatga aatcttaaaa tatattaagt atcctgttgt tttaaaaata
     7261 aattcttctg aaatattgca caaatctgat gttggaggag ttaaaatagg aattgagaat
     7321 agagatgagg cagtagatgc atataatgag attttaacta atgtaaaaaa agcaaaacca
     7381 gatgctaata ttgatggtat attagttcaa gaaatggtag aaagtggtat agaaattatt
     7441 attggtataa ctaatgatga ccaatttgga ccaatgttat tagtcggttt aggaggagta
     7501 tttgtagagg tatttaaaga tactacatta tatccattac ctataaatca tgatgaagct
     7561 attatgatgc ttaaaaaact aaaatcattt aagcttttaa atggctatag aggaagtgag
     7621 ccttgtgata ttgatgctct tgcagatatg atggtcaagt taggaaaata tgcttatgaa
     7681 aataaggatg aagtcaagga aatagactta aatccagtgt ttgtatatcc taaaggaaaa
     7741 ggcgtatgtg cagtagatgc cttaatagtt aaatataaat agtttataag ttttggtatt
     7801 caaccataat taaagcaccg actgaaaatc acattttgta agttagatag tgtgattttc
     7861 agtcacccca aatttaattt aaaaatattt taaaatgaaa gtatagtttt aagtttaatt
     7921 gtgaaatagc actgtataaa caataaaata ctacgtataa aacgaataat aaaaggtgtt
     7981 ttaatgataa gaatcaatct tgattttcat taaaacacct tctttattat attaaaaatt
     8041 tttaaacgtt atattgtcac aggtgggttt gttggagctg catatgtacg attagcaaat
     8101 tcttggtcaa caaagaatag accaccctct ccagcaagtt taacacgttt tatattgtct
     8161 tcacaatttg tttcttcttc tgcttgttca gatataaacc aaagtaagaa ttgagaagtt
     8221 ctaaagtctg attcagagtt tgctacagct gctaagttgt ttattaaagc tgtaaccttt
     8281 ttttcatgag ctaacgtacg ctcaagaata tccattgcag aagtaaagtc tgaatctggc
     8341 attgggatag catcaagctg aacctttcct cctacattat gtaagtattt gtaaaaaaac
     8401 atagcgtggt ctgtctcttc tttatattga acataaaacc aattagaaaa tcctttaagt
     8461 ccttctgctt caaggtaaga agacatagaa agataaagat acgcagaata taattcatgg
     8521 ttaatctgct cattaagtaa tttttccatt ttagctgaaa tcatttttat acctccaaat
     8581 tttaaatgct cttataaaat ataataatat taaatggaaa aatattcaat aaaagtgttt
     8641 aaatatttta catatttaat aaaaatttat actaccatta aaaaaattat aacttacaat
     8701 ggctatagag ccaattaatg gtgcatttgc tccaaaattt gaatgcctta cacttatttt
     8761 tttatattta gaaaaacttg ctgaagcagt caattttttt aataatgtat tttctataaa
     8821 tgtaccaggt atattaaagt catagcctat tattatagaa ctacaatcaa tcaatgtaag
     8881 tgtattaact aatgcatatg atacgtatgt acaaaattca tcaagcaaaa acatggcaag
     8941 actatcttta ttatttgcct catctattaa cttgactaaa gaaaaatctt tacagttaat
     9001 taatggggag ttaggataaa tatggctcaa tgattcaatt cttttaatta gattactgac
     9061 acttgtataa taatctaaac atccattatt accacaactg cacaatggtc cactaaaatt
     9121 aattgatgta tgtcctattt ctccactttg tcctaggttg ccagtatgaa gtttattttc
     9181 tagaactaga ccagctccaa taccattcat tatgtgtagg tatatataat ttgatatatt
     9241 tttacctaaa ccatacattt tttctgctaa tgcacccgaa ttagcatcat ttatcaaaaa
     9301 tattggcaaa tcagacattt cttttataaa atctactata tttaggtttg atacatttcc
     9361 aaaatctggg ggatttagta tggtaccatt tatatcatct actggaccta ttgaagaaat
     9421 accaattcca attatatctc tcttgttgat tttcattatt ttagtaaaca ttctttttat
     9481 tatatctttt aaaacatcat ttgatagaca tttggggtaa gtttcagaaa gttgttttac
     9541 aatattacct tttaaatctg aaataattac cttacatata tttcttctta ttaggattcc
     9601 acaaatacaa ggcgatatat tagaaatatc taataaaata ggtttacgac ctgatgaatg
     9661 tgcatttaaa gttgatgact ctatttccct aatcatattt tcattaataa gttcagtaac
     9721 tagatttgat aaagtcattt tagtaagtcc tgtagactta gctaaatcaa ctctggacat
     9781 aggtgtattt gtagaaagta atttcaatat tattaactta tttttaattt ttacatcttt
     9841 gatgttagaa ccttgtttgt tactcatcat aaatctcctt aaataaagtt aagtgtttta
     9901 tttattttca agtatactat caccttataa tagagtaaca tttttttact tgaaaatatc
     9961 attaattaga atatatcaca ttatatatca taaaatatat aaaaaaatgt tagaattaaa
    10021 ttaaaagtga aaagtagatt gatatataaa tagtaaatac ttaattaaaa aaagataatg
    10081 atagcaataa ttattgttac caagaatcta ctacttcaag tgttacctta ggtatttaag
    10141 ttgatagtgg tttgcacgta ataaacttga tgtaatatat attcttaata atatcgataa
    10201 aagatttact taaaaataat atagattatg taaacatata atctatttat tgtatacaat
    10261 aaaatttcat tttgtaaaac agtgaattaa atatgtaata tagatatcga tttttattac
    10321 aaattgttac taaatatagg tacattagaa caaaaatcta gtaaattgta actaaaatgt
    10381 aaatttttcc tttaatctat acaatattat ggaaactttt tgtaatttat gatataataa
    10441 caaatatata attattaaat tataataaat gactatagcg tagatgtaat actatgttat
    10501 agtcattttt gaattaaact tgatatatca agggggaaga atatgagaac aattagaaga
    10561 gttttagcat taggtttaac attagctata tttctaataa atgtacctaa tgtagatgca
    10621 ttaacatcag atacaattaa aggtaacaat atatatgaga cggctggatt aatagcagat
    10681 aaaaagagtt atgatacagc aattatggta aatatggata attctatagc agatggtctt
    10741 tcagcaagtg gtcttgcagg tgctgttgat gctccaattt tgcttgcaca aaaaaataag
    10801 ataccaaatg aaacaaaaca aagattaaaa aatgttaaaa agatatacat aataggtaaa
    10861 gaattatcaa taagtaagtc agtagaaact gaactaaaaa acacaggggc acaggtaact
    10921 agattgggtg gagatgacag aataaagact agttatagtg ttgctaaaga ggtaaatggt
    10981 atcaaaaaag ttgatgaagt gatattaact aatgcatata aaggagaagc agatacaata
    11041 agtgctgcac ctgtatcagt aagagatata gctcctatag tacttacaga tggaaagagt
    11101 gtaccttttt caacaagcaa tgtaaaaaca tatgcagtag gtgggagtat atcaatgagc
    11161 actagtttag ttaataaaac taatgctaaa agacttggtg gttcagatag atatgataca
    11221 aataagaaag ttataaaaga gttttatcca gatgcatcag aattttattt gagtgatgga
    11281 tatgatttag taaatgcact tacaggttct acaattgcta aggaaaatcc aattgtattg
    11341 gtgtcagaaa gtagtgataa gtctatatta gcaggagcag ataagattac tagattaggt
    11401 tcaataagtg acagtgtgta taataagtgt gtttctgctg cacaaaataa tggcgattcg
    11461 tctacaaaag gtgagtcacc tatgaaaaat gagacaagca tattaggtca gccaacagct
    11521 agtttagaag cgtgcttaaa atgggcaaaa tctaaaaaag ctaatgattt atttatagag
    11581 ttaataccaa tattatatga tactgctgtt caggaaggtg ttaatcctgt tttggcagta
    11641 gctcaatctg caaaagaaac tggtttttgt aattttggtg gagtattaga tgcatcattt
    11701 aagaatcctt gtggacttaa aacttctgta ggaggttctg atactgataa gaatgcccat
    11761 tcaagatttg atacttggga agaaggaata ttagctcaaa ttcaacattt atgcttatat
    11821 gcaggacaag atggttaccc actttcaaat ccagtagacc ctagacatga aaaatcttta
    11881 tttggtaagg ctaaaacagt tgaaagttta tctaataatt gggctggagg tcaatatgga
    11941 caagatttag taagaatgat gggagaaatt gaggcaacaa aataattgaa gattatcaaa
    12001 tatccaataa ataaggattg attattatga aaaagagtaa tatttttagt atcatatttc
    12061 ctacagtcat aatggtgcta atgtttttaa cttttgctac agatatattt aatattccag
    12121 atattcaatc taaaagtatt tttgttgtgg gaatggtttt gatttttcca atttcttttt
    12181 tgatacaagg gataatttgt gtattaaata agactaattg gattttatct cttattgtgt
    12241 ctttagtaac ttatatagta ttaatgtata tatttttaaa tgattctgca tatatatatg
    12301 ttttacttta tacagctttt tatatgattg gacatataac agctaaactc tattacaaaa
    12361 ttaagacttt taaaaattaa attgaagtat ataaaagtaa cattaggaga agtatcctta
    12421 atgattttct aaaatcagaa ataggtgctt ctcttttttg taaaaataaa agtatgtaaa
    12481 taaaatttaa ccattataat tttcttttaa caaatgtata caataaactt gttgtataat
    12541 taagatagat aaaataaagg gagggataat atgaatttaa tagataatca tattcatact
    12601 aatttttcaa gtgatggaaa ggattccatg gaagatacaa taaaaaaagc tataagtatt
    12661 ggtgttagat atttgacttt tacagaccat ttagaacatg atgaagatag aggattttgc
    12721 ataaattata atgactatgt tccagtattt aataagttta aagaaaaata taaaaaggac
    12781 atagagttat tactaggtgt tgaagtgggt tacagaaaac atctaaaaaa tgaaatagaa
    12841 gaaataataa attctaatcc ttttgatttt gtattgtgct caactcatac aattgacaat
    12901 gtacctgttc catcaaagga gtattttaaa ggtcttagca aagaagatgc gtattataaa
    12961 tattttaata gcatacttga aactaatcgt gaatttggag attacaatat atatggtcat
    13021 ttggattata tatctaggta tggaatatat tcagataata gagttatata caatgacttt
    13081 aaagatgtta ttgatgaggt cttaaagtca ataatcaata atgggagtgg tatagagcta
    13141 aatacttctg gttatagata tggattaaat gctatacatc caaatgaaga tattctaaaa
    13201 agatataaag aacttggagg atttatagtt actgtaggtt ctgattctca tagggtagaa
    13261 gatatatgta aagattttga tgtggcctat gatatgctta agtatcttga ctttaaatat
    13321 gtatctttat ttaaagaaag agaaacttat tttgtaaata tagaaaaagt taaatctaac
    13381 aatatagctt agagtgtgtt ttgaagtaaa ttaatggata gttaaaaagt taatagttat
    13441 aatattgtat cctctagaaa aatactagag gtttttattt tgctaaaata actaaataga
    13501 attaaattta aatagtatag tttatt
//
LOCUS       DAMJWU010000098        13454 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMJWU010000098 DAMJWU010000000
VERSION     DAMJWU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.102"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..446
                     /locus_tag="PC460_002959"
     CDS             <1..446
                     /locus_tag="PC460_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2601830.1"
                     /translation="KLMEKMTEARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAG
                     TSLNRQTTDIDTVKIIKVPSNLMKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSY
                     QFSKLDEPTAVTEGKYFYFEESFEDVFILNKKADAIQFVVEGAGA"
     gene            446..679
                     /locus_tag="PC460_002960"
     CDS             446..679
                     /locus_tag="PC460_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601831.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKVVRYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            689..1090
                     /locus_tag="PC460_002961"
     CDS             689..1090
                     /locus_tag="PC460_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601832.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEADMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTAYGKNKILTFRCLRW"
     gene            1084..1431
                     /locus_tag="PC460_002962"
     CDS             1084..1431
                     /locus_tag="PC460_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601833.1"
                     /translation="MVSKTRKAIEMLYRYKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            1431..1856
                     /locus_tag="PC460_002963"
     CDS             1431..1856
                     /locus_tag="PC460_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2601834.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            1849..2286
                     /locus_tag="PC460_002964"
     CDS             1849..2286
                     /locus_tag="PC460_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601835.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEAKNFEINEIAEMLLEELEYIEINGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNNADKMNTVELFGGLKRGDNFE"
     gene            2279..2455
                     /locus_tag="PC460_002965"
     CDS             2279..2455
                     /locus_tag="PC460_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601836.1"
                     /translation="MSKTLSKGTDYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            2456..3766
                     /locus_tag="PC460_002966"
     CDS             2456..3766
                     /locus_tag="PC460_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2601837.1"
                     /translation="MALGGGTFVTQNKVLPGSYINFVSATRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CSIATARCSGIRGNDLKVIVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITELQDNDYV
                     TWKKEATLEATAGLTFTNGTNGEAVTGTEYQAFLDKIESYSFNALGCLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKIKDTGLLESSLIYWTTGAIAGC
                     DINKSNTNKRYDGEFDVDVNYTQIHLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     EKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            3788..4258
                     /locus_tag="PC460_002967"
     CDS             3788..4258
                     /locus_tag="PC460_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2601838.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            4311..4568
                     /locus_tag="PC460_002968"
     CDS             4311..4568
                     /locus_tag="PC460_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601839.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            4573..5013
                     /locus_tag="PC460_002969"
     CDS             4573..5013
                     /locus_tag="PC460_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2601840.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPVLNKKGKATGQYTSETDFNTYTLKLCVETVVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            5334..5495
                     /locus_tag="PC460_002970"
     CDS             5334..5495
                     /locus_tag="PC460_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601841.1"
                     /translation="MDNFLLSILASLIASLIGYIVCRCIKNVKSHSTRGKSKSGWELG
                     FKIKFRKFK"
     gene            5644..5877
                     /locus_tag="PC460_002971"
     CDS             5644..5877
                     /locus_tag="PC460_002971"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601842.1"
                     /translation="MNKDIELTEKDLYCIARHIQVYVFKKDDEVIKKEDNPCCRCKHK
                     FKYYENSVCIYHCSVFQKLSKITGLKMGFGVKL"
     gene            5937..6464
                     /locus_tag="PC460_002972"
     CDS             5937..6464
                     /locus_tag="PC460_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDF2601843.1"
                     /translation="MREGKRRRGCLFWFIIIIIFSGVVGAIAGNSTNNGSTEKQKEDL
                     TKYIGEEGNIGDLKLTVNSISKASEIPVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDNSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            6607..7167
                     /locus_tag="PC460_002973"
     CDS             6607..7167
                     /locus_tag="PC460_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2601844.1"
                     /translation="MFCSNCGAEITGIGKFCSSCGVAVETEIIEDNNIKSNDLIVDAN
                     GIEINMTEIYRKYRKEKVNAIKNVMEISGLNIKEAKKIVDFSFEELKINFIDDTMSNS
                     EKEKIIQNQNRKNNIEKAQQESVACCPKCGSTSLTAQKKGFGIGKAMVGASLTGGIGL
                     VAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            7245..9533
                     /locus_tag="PC460_002974"
     CDS             7245..9533
                     /locus_tag="PC460_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2601845.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTTAMNIVLSSFEQLQRTSSNAVN
                     ANSIIRAREELARAEAGFDRLERQIRESDNQQRKLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLGMINDGQQSDAGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLSSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTFSQIWTSIKNNAIMIFGVIQKKIEQSMSSKGFRTFIDNFINSLYVLGNVAYNI
                     FNEIISILGSPFFQAFVNAIIVGVSLIVQALGWIITQALNIANVFAQNWSIIAPIVLG
                     VAAAMLVYNNALLLSIANKVKDIALSAKSLVMSFAHIVAESYRAAALVATTIAQDGLN
                     AAMAACPITWILYGIIAIVVAFFVAIAIFNHFAGTSVSAIGVVAGAISVAASFIGNLF
                     IATGNLIIDIVALIYNTLAGFAEFFANFLDDPIGSVIRAVSGMANAVLGIIRSIASAF
                     DTVFGSNLADAVSGWQDKLQGWTDKVAGEAKIKVERMDPNKLHFDRFNYGKAWDAGYK
                     WGDKLETNIKDKFDISKMAEDAKKKLGLDDLWDKKYGLGDGFGSAGLNSPLNDAAKGA
                     KDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVNIKIDMNNTNNISKDNDLD
                     GIVNVLTEKLNDAMAVSAEGIV"
     gene            9550..10251
                     /locus_tag="PC460_002975"
     CDS             9550..10251
                     /locus_tag="PC460_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2601846.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWAICQKQLGNGSLYKKVYELNKTMMDKANKGKK
                     LSKYTIYKGQVLRLV"
     gene            10244..12214
                     /locus_tag="PC460_002976"
     CDS             10244..12214
                     /locus_tag="PC460_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2601847.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFKLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLSLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKNEKQNGPAQSTTSKEDTDMANKINKLLKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKKNGDYMSFSSVDEGIKRGISNLSRNY
                     IHIGRKTLESIRNKYSSSSDKEWVKCVGAFYKQITGSTYNSNSAGTGVGSNEEAEKNL
                     KDLTYQVQNNNSNTSTNNNNKVSKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAFK
                     RGAGINLKRVSADQSKDSRGKLLCNINDVKAGDLVFFAYNKGKGNVHHVGLYIGNDQY
                     IHAPQTGDVVKISSLSGRQKKKHDFARARRFF"
     gene            12228..12488
                     /locus_tag="PC460_002977"
     CDS             12228..12488
                     /locus_tag="PC460_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2601848.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPMQIAFGTIESVNPLIVKIEQKA
                     SFEEFFLIQTETFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            12493..12912
                     /locus_tag="PC460_002978"
     CDS             12493..12912
                     /locus_tag="PC460_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2601849.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            12925..>13454
                     /locus_tag="PC460_002979"
     CDS             12925..>13454
                     /locus_tag="PC460_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2601850.1"
                     /translation="MTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTETYIAM
                     DELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPIDSRFNLGEYNYV
                     AIERISEGIYKMKCETAGPIFELGQLIPIEYIDKLETAELTEILINGEDEESEDSLRQ
                     RYYDSLNSQSFGGNMQ"
BASE COUNT         5358 a         1469 c         2667 g         3960 t
ORIGIN      
        1 ataagttaat ggaaaaaatg acagaagcta gagtacctga aaatggaaga atattgtatg
       61 ttactccagt agtaaataca cttatcaaaa atgcaaaaga gatacaaaga acagtaaata
      121 taaaggatgc aggaacttct cttaatcgtc aaacaactga tattgacaca gttaaaataa
      181 ttaaagtacc atctaatcta atgaaaactg catatgattt tacaactgga tggaaagtag
      241 gagcaggagc taaacaaatc tttatgtcct tagttcaccc aagtgcaata attacacctg
      301 tttcttatca gttctctaag ttagacgaac caacagcagt tacagaggga aaatacttct
      361 actttgaaga aagttttgag gatgtattta tattaaataa aaaagctgat gcaatacaat
      421 ttgttgttga aggagctgga gcataatggc acaagtaagg aaattaaata gaatattaac
      481 catagaagag tgtaaaatag atgatttctt agagatggga tatgatttga tagatgaaac
      541 tggtaaggta gtaaggtatg gcaagtcatt aaatgtaaaa gatttaatag ctgaaaataa
      601 tattttaagg tcaaaagttg agtctttaga agaagaaaat aagcagctta aagagaaaaa
      661 taaacttact aaaaagtagg tgaaaattat gggaaataat ataattgatg atatagaaaa
      721 aagacttgaa agttttggat atatattaaa agatggggat aagtggttaa tagattttgt
      781 aagagaaaaa atagaaaata ttattaaact agattgtaat ataaaaacta tgccaattga
      841 attgaaagaa attgaagctg atatgatagt tggagagttc ttatttacca agaaaaatat
      901 ggggcaatta gatatagaaa gcattaactt tgaagctgta gaaaagtcta tatcagaagg
      961 tgatacaaag gtagatttcg ctataggaag tggctctcaa acaccagaac aacgctttga
     1021 tagcttaata gcttatctta ctgcttatgg taagaataag atattaacct ttaggtgctt
     1081 aagatggtaa gtaaaactag aaaagcaata gaaatgttat atagatataa atgtactata
     1141 gttgagtatc agccaatcaa agaccctgta acaaaacgaa ctaacaataa agaagtgatt
     1201 gtattagaaa atcaaccatg caagctttca tataaaaata tagtttctgc tacagaagga
     1261 aaattagcta agctagagca aactattaaa ctctttatat ctccagatat agaaattaaa
     1321 gcaggttcaa aacttattat aaatgataaa gagtatgtaa gaagtggaga atcagctata
     1381 tatccaaatc atcaagaaat aatacttgag ttatttaagg ataaagcata atggctagat
     1441 ggggcagtgt tgattttaga gagtttaaaa gagtttgtaa aaagatggag aagcttacaa
     1501 agattgattt agataagttt tgcaaagatg cagcaagaga attagcagca agactcttag
     1561 gaaaagtaat tagaaggaca ccagttgata caggattctt aagacaagga tggaatggag
     1621 tggcttatgc taggtcgctt cctgtgtata aacaaggaaa taattatatt atagaggttg
     1681 ttaatccgac tgaatatgca agttatgtaa atttcgggca tagaactaaa gacggaaaag
     1741 gttgggttaa aggacaacat ttcttaacaa tttcagagat ggaactacaa agccaagttg
     1801 ataagattat agagaaaaag ttattaatat tacttaaagg agtatttgat gcttaataat
     1861 attatagatg gaatatcagt aaagttagat aaatcatttg gagaaaaata tacaatttat
     1921 agtgaggatg tagagcaagg tattaatgaa ccttgttttt ttattgttcc tttaaatcca
     1981 agcaagacac catatccaag cgggagagaa ttaaagaaaa attcttttga tgtacattat
     2041 ttccctcgtt cagaagctaa gaattttgaa ataaatgaga tagctgagat gttactggaa
     2101 gaattagagt atatagaaat caatggagat ttagttagag gcacaaatat gaattttgaa
     2161 attatagaca atgttcttca cttctttgtt gattataact acttcactat aaagagtaat
     2221 aatgcagata aaatgaatac agtagagtta ttcggtggtt tgaagagagg tgataatttt
     2281 gagtaagaca ttaagcaaag gaaccgatta caagtttact aaggagcaga tagttaattc
     2341 taagaagtat gtaaatagaa aagacttatt aaatgcaatt ttaaaagaaa atgagttata
     2401 ttccttctca gaagtagagg atagaataaa taaattcatg aaaggagtga gttagatggc
     2461 tttaggtgga ggaacatttg taacacagaa taaagtatta ccaggtagct atataaactt
     2521 tgtaagtgcc acaagggcaa ccagttcact tagcgataga ggtattgttg caatgccttt
     2581 agaacttgat tggggaattg atgaagaagt atttcaagtg actagtgatg attttgagaa
     2641 gtattcaact aagtattttg gatatgatta tacacatgag aagttgaaag gtttgagaga
     2701 tttatttaaa aatataaggc taggatattt ctataaatta aataaaggtg ttaaagctag
     2761 ttgtagtatt gctacagcta gatgcagtgg tataagaggt aatgacttaa aagttatagt
     2821 aacaacaaat atagatgata atgctaaatt tgatgttgta acacttttag ataataagaa
     2881 agttgatact caaatagcta aagttattac agaactgcaa gataatgatt atgtcacttg
     2941 gaagaaggaa gcaacattag aggctacagc aggattaact tttactaatg gaactaatgg
     3001 tgaagctgtg acaggaacag agtatcaagc ttttcttgat aagatagaaa gttactcatt
     3061 taatgcacta ggatgtttgg ctacaacagc agagattaaa agtttgtttg tagagtttac
     3121 taagagaatg agagacaaag ttggggctaa gtttcaaact gtattatata aaaagaatga
     3181 tgcagattat gaaggtgtag tatctgttga aaataagatt aaagatactg gattattaga
     3241 atctagctta atttactgga ctactggagc tatagcagga tgcgatataa ataaatctaa
     3301 tactaacaag cgatatgatg gtgaatttga tgtagatgtt aattacactc aaatacattt
     3361 agaagaagct ttaaaaactg gtaagtttat atttcataag gttggagatg aagttcatgt
     3421 attagaggat ataaatactt ttgttagttt tacagatgaa aagaatgacg acttttcaag
     3481 taatcaaagt gttagagtac ttgaccagat tgctaatgat atagcgactt tatttaatac
     3541 aaagtacttg ggtgaagtac caaatgataa atctggtcgt atctcgtttt ggaatgatgt
     3601 agttaagcat catgaacaac tgcaaaatat gagagcaata gaagatttca aagctgatga
     3661 tgtttctgta gaacctggaa gcgacaagaa gactgttgta gtaagtgatg ctgtaaaagt
     3721 tattagtgct atgagtaagc tttatatgac tgtttcagtt agttaacaat aagaaaggag
     3781 aataataatg gcacaacaaa taaaagcaag agatacaata agtgcatcta aggcagagtg
     3841 ttttgtaact ataaaaggta aaagatataa ttttatgcaa gctattaact tagaagctaa
     3901 aatggaaaag aataagagtg agatacctat attaggtagt actacaaaag gaaataaatc
     3961 aacaggaagt aaatattcag gaaatgcaac attttattat aatacctcta tatttagaga
     4021 attgttgtat gagtataaag aaactggtga ggatatttac ttcgatatac aaattaccaa
     4081 tgaagaccca acaagttcag tgggtcgtca aactataata ctggaagatt gcaatatgga
     4141 ctcaggcata attgctaaat ttgatgctga tggggagtat ttagatgaag atatggattt
     4201 tacttttgag aattggaaat tagttgagaa atttaatata gcaaatggta tggagtaaaa
     4261 tacacattta tgatttatgt atgtgtattt tttatttata agaataggag atgattaaaa
     4321 ttaaggataa atatgagata aaagattcaa tttcttttga ttatagcaat aaaagacctt
     4381 tggaagaacg tgtcagcgag atgtataaaa aggcaggaaa atatcttata gatatttcag
     4441 ataagttagc aacagataca attgatggtt cgtcattaaa gccaataatc ataaaatttg
     4501 aaataaatga agctggtgtt gcaacaatag aaaaacaaac aaaatatttg gtgatggagg
     4561 tagaataaga atatgggaga tttaaacgct tttttaagtc aaaatgcaat aaaagtagag
     4621 aatagaaagt atgtggcaag tgaaaggttt ataggagaag atggaaaagc aatcgaatgg
     4681 gaacttaaag caatagattc agatagagat agacaattaa gaaaagattc aactataaga
     4741 gtacctgtac taaataaaaa agggaaagca acagggcaat acactagtga aacagatttt
     4801 aatacttata ctttgaaact gtgtgtagaa actgtagtat ttccagattt acatgatgca
     4861 gaacttcaaa atagctatgg tgtaatggga gcagaggaac tattaacaac aatgttaact
     4921 cctggtgaat acacagacct ttcaagtgag gtaggagaag tgaatggttt tgataggact
     4981 tttgaagata aagtagaaga agcaaaaaac taattgaagg aggcgattat gatgctagtg
     5041 tagctcatta ttgccttcat aaattcaaat ggaaaccaca tgaatataca gatttaccag
     5101 acttcgagag ggcatttgtt gctgcttcta tagatattaa agtagaagaa gaaataaaag
     5161 aagaaaaaaa gattgctaaa gaagctagaa gaagtagaag aagataaaat ataggtaaaa
     5221 tatgtaagaa ttatatgtta taataattgt agcaagaaga tgtaatctac aatttataga
     5281 gtggagttca tactgggata aaacctactt cctaatgaaa ggaggtggga agtatggaca
     5341 attttttact tagtatatta gctagcttga tagctagttt aattggatat atcgtttgta
     5401 gatgtatcaa aaacgtaaaa agccactcta ctcgtggcaa gagtaaaagt ggctgggaac
     5461 ttggttttaa aataaagttc cgcaaattta aataatttat attttttaaa attatgaact
     5521 tcactctacc gctaaataga ttgtagttct tcttgccttt attataccac aaattagaaa
     5581 aaatatgcaa agtacttgtt ttaatagtaa gtactttttt tatgtgaaaa agaaggtgat
     5641 tgaatgaata aagatataga acttacagaa aaagatttat attgtattgc tagacatatt
     5701 caagtgtatg tgtttaaaaa agatgatgag gtcataaaaa aggaagataa tccatgttgc
     5761 agatgcaagc ataaatttaa gtattatgag aatagtgtat gtatctatca ttgttcagta
     5821 tttcagaagt tatctaaaat tacaggtctt aaaatgggtt ttggagtcaa actataatgt
     5881 aaaaattttg aatatatagt ataatattct tataaaatat aatttagggg gatattatga
     5941 gagaaggaaa gagaagaaga ggatgtctat tttggtttat cattattatt atattttctg
     6001 gggttgttgg agcaatagca gggaacagta ctaataatgg aagcactgaa aaacaaaaag
     6061 aagatttaac taaatatatt ggtgaagaag gtaatatagg agatttaaaa ttaactgtta
     6121 atagtatttc aaaagctagt gaaataccag tagcttctgg ttatttggcg tacactccag
     6181 atagtggcaa atatggtatt ataaatttaa caataaaaaa tcaaactaag gaaagccaat
     6241 cttttatgct aaattcattc acattaatag gaccagataa ttcaaaatat gttccatctt
     6301 tattgattga tgtaggaaat aaatatataa ctatggatac tgtaaatcct aatttagatg
     6361 taacaggtaa tattgcattt gaaattccta aggatttact agtttctgat tgtaaactaa
     6421 aatatagtgg ttcagagcaa gaatttatat taaaagaaaa ataatatata gatttaaaat
     6481 caaaaatact aaaaacactt actaatgtag gtgttttttt aatgaaagga tgtgatgata
     6541 atgtaaaaat tttactaata tagtataata ttcttataaa attattatac tgggggggga
     6601 aatgttatgt tttgttcaaa ttgtggtgca gaaatcacag gcataggcaa gttttgttca
     6661 agttgtgggg ttgctgtaga aactgaaatt attgaagata ataatattaa atcaaatgac
     6721 ttaatcgttg atgctaatgg aatagaaata aatatgactg aaatttatag aaaatataga
     6781 aaagaaaaag taaacgcaat aaaaaatgta atggaaataa gtggtttgaa tataaaggaa
     6841 gcaaaaaaaa tagtggattt ttcttttgaa gaattaaaaa ttaattttat tgatgatact
     6901 atgagtaatt cagaaaagga aaaaataata caaaatcaaa atagaaaaaa taatattgaa
     6961 aaagctcaac aagaatcagt tgcttgttgc cctaagtgtg gttcaacatc cttgacagct
     7021 caaaaaaaag gttttggtat agggaaagca atggtaggag ctagtctaac tggtggtata
     7081 ggtttagtag ctggaaattt aggagcaaag aaagttagag ttacatgctt gaattgtggg
     7141 aagcagtttt gggcaggcaa aaaatagatg tattaattat aaaaacactt actaatttag
     7201 taagtgtttt tattatctaa attaacagaa aggagagtga aaaaatggct acaatacaga
     7261 catcaattcg catatttgat ggaatgacac ctgcgtttcg gcatatgaca actgctatga
     7321 acatagtact gagttcattt gagcaattac aaagaacttc tagtaatgca gtaaatgcta
     7381 atagcataat aagagctaga gaagaactag caagagcaga agctggattt gatagattag
     7441 aacgacaaat aagagaatca gataatcaac agcgaaaact taatgaggat ataaataagg
     7501 gtgcaagttc tacagataga ttagttggaa gtgcaaagaa gctagcagca acttatttag
     7561 gtataagaac attaggaggt ctaggaaatt taagcgacca gatgacaagt actaatgcga
     7621 gacttggcat gataaatgat gggcaacaat cagatgctgg acttaataaa atgatatttc
     7681 aatcagctga aaggtcaaga gcatcttact tagatactgc acaaatagtg agtcgtatag
     7741 gcatgaacgc aggaaaggcg tttagcagta caaaagaaat tgtaggtttt gcagagcaat
     7801 taaacaaaaa atttgtaata gcaggcgcaa gtactgagga aatgaactcg gcattgttac
     7861 agctaaccca agggttgagt tctggcgtat taagaggtga ggaactgaat gctgtgtttg
     7921 agtcagcacc taacatcatc caatcgattg cagattattt ggacgtggac ataggaaaaa
     7981 taagaggaat ggcatcagag ggaatgttaa cagcagatat tgtaaaaaac tcattacttg
     8041 cagcagcaga gcagaccaat gcagagtttg aaaaaatgcc ttacacattt tctcaaattt
     8101 ggacttcaat taaaaataat gcaatcatga tatttggtgt tatacagaaa aaaattgaac
     8161 agtctatgtc tagtaaggga tttcgaacct ttatagataa ttttataaac tctttgtatg
     8221 tacttggaaa tgttgcttat aacattttta atgaaattat aagtatatta gggagcccgt
     8281 tttttcaagc atttgtaaat gcgattattg taggtgttag tttaatagtg caggcactag
     8341 gttggataat aacacaggca ttaaatattg ctaatgtgtt tgctcagaac tggagtatta
     8401 ttgcaccaat agtacttgga gttgcggctg ctatgttagt gtataacaat gcgttattac
     8461 ttagtattgc gaataaagtt aaagatattg cactatctgc caaatcttta gtcatgagct
     8521 ttgcacatat agtagcagag tcttatagag cagcagcttt agtagcaact acaatagcac
     8581 aagacggatt aaatgcagca atggcagctt gtcctattac ttggatttta tatggaatta
     8641 tagctatagt tgttgcattt tttgtagcta tagcaatatt taatcacttc gcaggcacta
     8701 gtgtatctgc tataggtgta gttgcaggtg caatatcagt tgcagcctct ttcataggaa
     8761 acttatttat tgcaacagga aatttaatta tagatattgt agctttaata tacaatactt
     8821 tagcaggctt tgcagagttc tttgctaatt ttttagacga cccaattggc tctgttataa
     8881 gagcagtatc cggaatggct aatgctgtat taggcattat aagaagtatc gcaagtgcat
     8941 ttgataccgt gtttggttca aacttggcag atgcagtaag tggatggcaa gataaattgc
     9001 aaggttggac tgataaagta gcaggagaag ctaaaataaa agtcgaaaga atggacccta
     9061 ataaactgca ttttgacaga tttaattatg gaaaagcatg ggacgcagga tataaatggg
     9121 gagataagtt agaaactaat ataaaagata aatttgatat tagcaaaatg gcagaagatg
     9181 caaagaaaaa attaggattg gatgatttat gggataaaaa atatggatta ggagatggat
     9241 ttggttcagc aggattaaac tctcctctca atgatgcagc aaaaggagca aaagacactg
     9301 cgggaaatac agcaaagatg gctaaaacaa tggataaaag ccaagaagac ttaaaatact
     9361 taagagacat agcagaacag gaaacaatca atagatttac aggagtaaac ataaaaattg
     9421 atatgaacaa cacaaacaac ataagtaaag ataatgactt agatggaata gttaatgttc
     9481 taactgaaaa gttaaatgat gcaatggctg tttcagccga aggaatagtt taggaaggag
     9541 agtgagaaaa tggcttatga tttttaccta gatggagtac aactaccaat cgcaccacca
     9601 aagcttgaag tcaaagtgac aaataaaaac aagacagttg atttaataaa tgttggagaa
     9661 gtaaatatat taaaaaaaga aggattatct gaaataagtt ttgaagcaga atttacacat
     9721 aataaactac cattttatcg tggaactttt agggatgttc aattcttttt aagtaaactg
     9781 gaactactaa aaactgattg taagccattt caatttattg tatcgaggga attaggtaat
     9841 aaagtactat ttaacactaa tataaaagta tctcttgaag agtatgctat ttcagaagat
     9901 gcagataatg gctcagatac aaaagttgca ataaaattaa aacaatatag agattactca
     9961 actaaaaagt tagttcctgc aactcctgaa aagacaaact atggtaggac tccccctcca
    10021 gtcatgaaac caaaagaatt tagaccagat tcatccaata agccaaatgg taaaacatat
    10081 acagtaaaag caggggattc tctttgggca atttgtcaaa agcaattagg aaatggttcg
    10141 ttatacaaga aagtatatga gttaaataaa acaatgatgg ataaagctaa caagggaaaa
    10201 aaattaagta aatacaccat ctataaaggg caggtgttga ggcttgtctg atgatttagt
    10261 tctggcgaat gatagagata taaggctagt tattgcacat tgggaagatt tctacgaacc
    10321 tgtagttttg gatggtatca catgggaaat agagagacga ggaacaccat ctaaacttga
    10381 atttacaata gtcatggatg atatattaga gttttgtgaa ggtaactctg taagactata
    10441 ttacaaaggt gtaggtatat tctatggata tatatttcag aaaaagagag ataaagaaaa
    10501 tcatatcaag atagttgctt atgaccagct aagatatttt aagaataaag atacttatgt
    10561 atatagtaat aaaacagcaa gtgaacttgt aaagatgttg gctaaggatt ttaaattaaa
    10621 atacaatgtc atagaagata caaaatataa aatatcgaga gttgaagaaa ataaaacact
    10681 ctttgatatg gtcttaactg cactagatga tactctaaga gagaaaaagg aaatgtatgt
    10741 tttatatgat gattttggaa gaataacatt aaagaatgtt gcttctatga aacttgatac
    10801 ggttatgaac aatgatgtaa ttgaggactt tgactacaat tcttctattg atagtgatac
    10861 ttacacaaag attaaacttg tgagagataa tgaagaaaca ggaaaaagag atgtgtatat
    10921 tgctcaagac tcaacgcata tgaggagttg gggaatacta caactatttg atacagttga
    10981 taaaaatatg agtgaagcag agataaaaca aaaatgtgat atactcctaa aactatataa
    11041 taagaaaact aagtcattaa gtttaaaaaa tgtgttagga gatattagag taagagcagg
    11101 ttgtttagta cctgtttttt tgtcgttagg agatattgat ttacaaaatt atatgttagt
    11161 tgagaaagta aaacatacat ttgaaaataa cagtcatttt atggacctaa ctttggttga
    11221 tggagacgaa tttgcttctt attcttcatc aagttatagt agtggaaata ctaataataa
    11281 aaatgagaaa caaaatggtc ctgcacaaag tactacaagt aaagaagata ctgatatggc
    11341 taataagatt aataaactac ttaaaggtaa attatcaaat acaggaaata tatttgttaa
    11401 atattcaaat gcttataaag ttaatccagc actcatggct gctatatcta tgcacgaatc
    11461 agctagaggg acttcaaata ttgcaaatac taaaaataat ttctttggaa tgaaaaaaaa
    11521 tggagattac atgagttttt ctagtgtaga cgaaggaata aaaagaggta taagtaattt
    11581 atcaagaaac tatatccata taggacgaaa aactttagaa agcatcagaa ataaatattc
    11641 ttctagttca gacaaagaat gggtaaaatg tgtaggtgca ttttataagc aaataacagg
    11701 aagtacttat aattctaata gtgcaggcac aggagttgga agtaatgaag aagcagaaaa
    11761 gaatttaaaa gatttaactt atcaagttca aaacaataat tctaatacat caacaaacaa
    11821 taataataaa gtaagtaaag ttattcaaga agcaaaaaat caacttggca agccttacaa
    11881 atggggtggt aatggtccaa agagttttga ctgtagtggt cttatggtgt gggcatttaa
    11941 aagaggtgca ggaataaatc tcaaaagagt ttcagcagac caatcaaaag atagtagagg
    12001 aaaactatta tgtaacataa atgatgtaaa agctggtgat ttagtattct ttgcatacaa
    12061 caaaggaaaa ggaaatgtac atcatgttgg actatatata ggaaatgacc aatatattca
    12121 tgctccacaa actggtgacg tagtaaaaat aagtagttta agtggtagac aaaagaaaaa
    12181 gcatgatttt gcaagagcta gaagattctt ttaagtgagg tgataaagtg tcacaagaat
    12241 tattgcaaat aattaagaag gctgcaatgg atgcagtaga aacaagcaat ccaatgcaaa
    12301 ttgcatttgg aactatagaa agtgttaatc ctttgatagt taagatagaa caaaaagcat
    12361 cttttgaaga attttttcta atacaaacag agacttttaa aagatataca gataaaaaaa
    12421 taggggataa attagtctta attaggatgc aaggaggaca gcaatatttg attttagata
    12481 ggatgtgatg aaatgttacc aacagataac attgactatg atatagaaga tgtatcgata
    12541 attaattttg atgttagaca agaaccaagt aagacgttta aattgaatat agagaaaaat
    12601 agagtagatg gtatttgtga tgatgtagaa gcattaaaac aaaccatttt tttaatttta
    12661 aatactgaaa ggtatgagca ccttatttat tctagaaatt atggtgttga attaaatgat
    12721 ttaattggag aacctatttc atatgtaata cccgaacttg aaagaaggat aacagaagca
    12781 ctaattcaag atgataggat tgaaaatata gataattttg agtttcaaaa tataaagggt
    12841 aaagtacaat gtagattttc agttcataca aaatatggaa atataaaagc agagaaggtg
    12901 gtgagtgtat aattgtttga gttaatgaca tttgaaaata taattaaaag aatgttagat
    12961 agtgtaccag atacttttga taaaagggaa ggttctataa tatataatgc tcttgctcct
    13021 gttgctatag aacttacaga aacatacatt gctatggatg aattactaga ccaaacattc
    13081 gtagatactg ctagttatta ttatttagag aagagatgta aagagagagg tattacacca
    13141 cttgaagcca ctaatacaat tgcaaaagga gtttttaaca tagatattcc tattgattct
    13201 aggtttaatc taggagaata taactatgta gcaattgaga gaatatctga aggtatatat
    13261 aagatgaaat gtgagactgc gggacctatt tttgagttgg gacaactaat acctatcgaa
    13321 tatatagaca aattagaaac agcagaactg acagaaatac tgataaatgg agaagatgaa
    13381 gagagtgagg atagtttaag acaaagatat tatgatagcc taaattcaca gagctttggt
    13441 ggaaatatgc aaaa
//
LOCUS       DAMJWU010000099        13407 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMJWU010000099 DAMJWU010000000
VERSION     DAMJWU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.103"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            132..545
                     /locus_tag="PC460_002980"
     CDS             132..545
                     /locus_tag="PC460_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2601851.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
     gene            736..1953
                     /locus_tag="PC460_002981"
     CDS             736..1953
                     /locus_tag="PC460_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF2601852.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNSPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            2115..2813
                     /locus_tag="PC460_002982"
     CDS             2115..2813
                     /locus_tag="PC460_002982"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF2601853.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(3033..3650)
                     /locus_tag="PC460_002983"
     CDS             complement(3033..3650)
                     /locus_tag="PC460_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2601854.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(3777..4799)
                     /gene="argF"
                     /locus_tag="PC460_002984"
     CDS             complement(3777..4799)
                     /gene="argF"
                     /locus_tag="PC460_002984"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF2601855.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            complement(4908..6095)
                     /locus_tag="PC460_002985"
     CDS             complement(4908..6095)
                     /locus_tag="PC460_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF2601856.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYVERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            complement(6125..6985)
                     /gene="argB"
                     /locus_tag="PC460_002986"
     CDS             complement(6125..6985)
                     /gene="argB"
                     /locus_tag="PC460_002986"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF2601857.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            complement(7000..8217)
                     /gene="argJ"
                     /locus_tag="PC460_002987"
     CDS             complement(7000..8217)
                     /gene="argJ"
                     /locus_tag="PC460_002987"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF2601858.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEAHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(8258..9292)
                     /locus_tag="PC460_002988"
     CDS             complement(8258..9292)
                     /locus_tag="PC460_002988"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF2601859.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     misc_binding    complement(9546..9759)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9977..10744)
                     /locus_tag="PC460_002989"
     CDS             complement(9977..10744)
                     /locus_tag="PC460_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF2601860.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     gene            11043..11516
                     /locus_tag="PC460_002990"
     CDS             11043..11516
                     /locus_tag="PC460_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2601861.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(11885..12442)
                     /locus_tag="PC460_002991"
     CDS             complement(11885..12442)
                     /locus_tag="PC460_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2601862.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSFL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            12700..13398
                     /locus_tag="PC460_002992"
     CDS             12700..13398
                     /locus_tag="PC460_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2601863.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNIPTKILYGKHDELTEIGVVEKFVEKFNCNLTIVENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT         4562 a         2141 c         1488 g         5216 t
ORIGIN      
        1 attaagaata agtattatta gtttgacatt agcataaatg cctattagaa taatgttaaa
       61 ctatattctt tctactgatt ttattaaaaa ataccaatat acaaaagatt atacaaggag
      121 aaagttcaaa tatggaatta gaaattacag aaaaaataga aaaacaagat gaagatacta
      181 tttttcaagg attattaaaa tataatcttg ctagacttga agataaaaac ccagtggatt
      241 taggaatata tattcgagat aaagcaggta aaattgttgc aggttttata ggtgttactc
      301 atggtaattg gctttctata aaatacttat gggttagtga gaagcttaga tataaaggta
      361 ctggaagtca acttttatat aaggctgaaa aaattgctaa agaacgaggt tgtaaatatg
      421 tttttttaga tacattcagt tttcaagcac ctaaatttta tgagaaattt ggataccata
      481 aagtattttc acttgaaaat tatcctctaa ctggaaagag atattatttt agaaaaatac
      541 tataataata aaaatcatat tttatatttt taacttaaaa cttctgctct tcgtctacag
      601 actttatatt tttatatcat tattaatact aaaatagtat tttactatac atacaaaatt
      661 atttgaaagt tcaaaaatta taagtataaa agctaatctt agcttaaata aaaattatat
      721 ttttaggagg taaatatgtt aggaaaaaaa tgtatggatt atctacaaac attaggaaaa
      781 ataagttcaa caacaaatgg tcttacaaga cttatcttga ctcaagaaca taaaaaatct
      841 attgatttaa ttagttcatg gatggaaggt ttaaatcttg atattgaaat tgatgacatt
      901 ggaaacgtta taggtacata taaatcaagc tttccaaatt ctcccacatt agtagttgca
      961 tctcatcaag atagtgtaaa gtgtggaggt atatttgatg gtatgttagg tataatagtg
     1021 cctctagttg gtcttgaaga agcaaaacac aataataggt cttatccttt caatattaag
     1081 ctaattgctt tcgctgaaga ggaaggaact agatttgaaa cttctttaat gggaagtaaa
     1141 gtttttgctg gtacttttaa agaggaactt cttaaatctg ttgatgaaaa tggaattaca
     1201 cttgaagaag ctgtaacaaa gtttggattt aatacaaaaa acttaacaaa tttgcatcct
     1261 agaaaagatg tagatgcata tttagaattt catattgagc agggtcctgt tttagaaaat
     1321 gaatctcttc ctgctggaat tgtatcaagt attacaggat ttaaaagttt taagatatca
     1381 gtaaatggta agtctggaca tgctggtaca cttccaatga atatgagact tgatgctgga
     1441 tgttgtgcct gtgaatgtgt attagctatc gaaaaagtag ctaaaacgac tgctgatttg
     1501 gttgctactg taggaaagat gaatttctat ccatcttcat caaatgtggt acctgaaaga
     1561 gctgaattta ctcttgatgt acgctcttgt tcacaagaaa ttcttgataa ttctgtagaa
     1621 aagatattta atgaaatatc acatatatgt gaaaatagaa aacttaatta tacaagtgaa
     1681 cttgcttttg aaaatgttcc tgtaccatgt tctaataaaa taacaaaaat catagaaaaa
     1741 agttttatag atttaaatct taatcctttt tatatttata gtggtgctgg ccatgatgct
     1801 caggaaatgg ataatataac tgatattggt atggtattta taagatgcgc tgggggcgta
     1861 agtcataatc ctaatgaaag tgtttctgta gatgacttgg atactgctgt aaaaatattt
     1921 ttaaaaatac tagataatct tgatttaaaa taattcttat ataagaaagt atattaaaat
     1981 atataatcta aaaaatcact ttatatttat attatataag tgtacaatat aatctattat
     2041 aaaatataat tttatgcttt tatagatttt tattttatac ataaatatat ttatatataa
     2101 aggagataat ttttatgaat gaaaaattaa ttggtatttt ggctggaatg ggtccaaggt
     2161 caacagcccc ttttgttgat ttaattattg atgagtgtca atctcaatat ggagcaaaat
     2221 atgatgatga atttccaaag atgatgatat attcattacc taccccattt tatattgacc
     2281 gtccaataaa tcatgaactt atgaaagaaa ctataattga tggattacaa aagcttgaat
     2341 caactggtgt aaattttatt gcaatgccat gcaattcagc acatatatac tttaaagaat
     2401 taaaagagtc tattaatata ccaatattaa atattgtaga agaaacagtt aaaaaattac
     2461 ctactatctc acaaaaagta actctttttt cgactagttc aacttttgag tctacaattt
     2521 accagaatgg tataatacat aatggacatg aatttatatt taaagatgaa tggcaaataa
     2581 aattaaacaa tcttattcaa aatataaaaa tggataaaga aaatccacac aatattgata
     2641 tttggaataa acttatcgaa gatgttaaaa atgaatctat agaaaatata gtaattgctt
     2701 gtactgattt aaatgtagtt ttagaaaaat catatccttc aataaatatt gttgattcat
     2761 caaaatgctt ggctgaatca gttgtcaata aatatctaaa attagttaaa taaatttttc
     2821 atgcactata ttataaaaaa tatatgttat aagtttattt taaatagtat agacttataa
     2881 catatatcat agtttcttga caatatagaa attaaccatg tatttcatta tattaataaa
     2941 aaggatacca aaaatggtat ccttcataaa tatttatatg tttatttata aaaataatca
     3001 tatatttatt actttactac acctaaacaa tattattcaa gtgtattcat agagttagaa
     3061 aattcttgtc ctgctgtaac aagattagat aacaatacat cagctactgc atcatttact
     3121 gaacttatta tccatttatt atccttttta gaacattcaa actctacatt ttttgtaaca
     3181 taagtctctt taacgctttt ttgtttttct ttcataattg atactaacat ttcagttgtt
     3241 ttctcatcag ttaagtcttg tcctgaaaaa ctcataccaa tcatctttgt aaatgcctca
     3301 gctactattt cttgaagtaa tggtgttgaa tctataaatt tacattctac tttgattgta
     3361 gccttatcac ctttaacctc gctgtctttt attgtatatg tgattttaga tgcattctgt
     3421 tttagataat ctaaaacata ttgggcattt gggtcttttg tatctaattc ctttaatata
     3481 tccttatatt tttcgtcatt attttccatt accttattca tgccttcaaa gtcaaacttt
     3541 ttcgcactgt taaaaaaact atcaattgta tcttctggtt ttgcacttga acatgctgtt
     3601 aaagatattg ctaataaaaa agttgttaaa gctaatatga tttttttcat atctcaaatc
     3661 ctccctctta atttgttgat acataagtta ttttaacata taatgagagt ttttgaataa
     3721 tatatttaaa atattttata aattttcatt aaagtaaagt ctatattaaa acatatctat
     3781 attaaattat tgttcatatt tacatcagtc atagtagaaa ccataacagc ttttattgta
     3841 tgcattctat tttcagcttc ttcaaatacc tttgaatatt ttgaattaaa tacctcgttt
     3901 gtaacctcta attctgataa tccgaacttt tcataaatct cctgagcaac cttagtttct
     3961 aaatcatgaa atgctggaag acagtgtaaa aatataacat tttcattttc agttttcttt
     4021 atcatatcca tatttacttg ataatcttta agttgattta ttcttgcttc atattgctcc
     4081 tcttctccca tagaaaccca tacatctgta tatataacat ctgcaccttt aacatcatca
     4141 atattttcag tcaacattat atctcctttg gatttcttgg ctatttcctt catttcctta
     4201 accaattctt catttggcca aagttcctta ggtgctagtg ctacgaaatg tattcccatt
     4261 ttagcacatc ctatcatcag tgagtttccc atattatttc tagcatcacc aacatataca
     4321 aatttaactt tattcaaatc tttatctaca ttttcaatta ctgttaaaaa atctgccagt
     4381 atttgtgttg gatgatattc atctgtaagt ccattccaaa caggcacacc agaatatttt
     4441 gctagacttt caactgtttc ttgtttaaag cctctaaatt ctattccatc atagaatctt
     4501 cctagaactt ttgctgtatc tgatacagac tcttttttac ccatatgaca atcatttgga
     4561 cctagataag ttacatgtgc tccttcatca tgagctgcta cttcaaatga acatctagtt
     4621 cttgttgatg ttttctcaaa taataaagct atattttttc cttgtagcaa atcgcctttt
     4681 ataccaactt ttttcttagt ttttaaataa cttgataaag ttaataaata tctaatctct
     4741 tcttttgtaa aatcctttaa tgtcaaaaaa ctcttacctt ttaaacttat attattcata
     4801 ttaattcgcc ccttcttata aactttttaa attcaattaa acttgttagc actttacttt
     4861 tttatttact tctaattttt atctttaatc aactatttct tgaatattta gttaatttct
     4921 agtaatatat cttttaggat ttcaagagcc tcatctattt ccttataact gattgttaat
     4981 ggtggtaaaa atctcaatac attttttcca gcagtcaaaa taagcaatcc tttttttcta
     5041 gctttctctt ctataatact agattcaata ttagtttcaa ttcctatcat tagaccaata
     5101 ccttttgtct ttaatacaac tttgttatta aaactttcaa tagattttct tatataattt
     5161 cctttttcta atacttcatt ataaaaacca tctttagata tctcatctaa tacaactgaa
     5221 gctgccacca tagctaataa attacctcca aaagttgtac cttggtctcc ttttcctagt
     5281 acttttgaaa gttttttatt tactaatact cctcctactg gtattcctgc tcccaaccct
     5341 tttgctacgg taactatatc tggttttaca tcaaaatatt catagccaaa aagtttacca
     5401 gttcttccta taccgcattg gacttcatca aaaatcacta caatatcctt ttcttgacat
     5461 atttttacta tttccaaaac ataatctttt tcaagaacat taactccacc ttcgccttga
     5521 atcgcttcaa gaattattgc acaaactgtg gaatcaagat tattttttaa atcttcaata
     5581 ttatttcttt ctacatattt aaaaccttcg ggaagaggat aaaaatactt atggtatttg
     5641 tccataccag ttgccattaa agacatcatt gttctaccat gaaaagagtc ttttaaactt
     5701 atgatagttc ctctaccatt accatatttg tcactactat actttcttgc tattttgaat
     5761 gctgtttcat ttgcttctgt tccagaatta caaaaatata ctttagccat tccacttgaa
     5821 ttaattattt tctctcctga atacaacact ggctcattaa aatataaatt tgaagtatgt
     5881 aataatttat ctacttgctc ttttagagca ttctttaaat tactattact atacccaaga
     5941 gaagatactc catatccact tgtaaaatct atatatttat tgccattagt atcaaaaaaa
     6001 caacttcctt caccataatc tatgacaaaa ttgggttgat tatatgtatc tataaaatat
     6061 tcattccatt ttgaaattgt attgtcgtta ttcattctta ttctcccctc ttaaacttat
     6121 aattttattc attttctttt cttattaatg tacctattcc actatcagta aatagctctg
     6181 ttattataga atgtggaact cttccatcca aaatatgtac cctatttact ccgttattta
     6241 aggcatctac gcatccctct attttaggta tcatacctcc agtaattata ccttcttcaa
     6301 ataatttatc aacatcctct aatataactt ctgttattaa tgatttttct tcatctggct
     6361 ctcttaacaa accagggaca tctgtaagaa gtatcaattt atctgcattt aactctttag
     6421 ctatagcaga agctgctgtg tcaccattta tattataagt ctctccatca tctccaacac
     6481 ctatagttgc aattactgaa atataacctg actttagaca agattctata agctcaacat
     6541 ttactttttt gatttctcct acaaatccaa gttcataatt tttatatggg tcacataaaa
     6601 tcatattatt atctattcca caaagcccta ctgcttttcc accttttgta tgtattttat
     6661 ttactaggtc tttattaacc tttccagcaa gtaccatttt tacaatttcc atggtctctt
     6721 catctgtata tctaagacca tttacaaatt tactttctat atccactttt gctagcatct
     6781 tattgatttc tgcccctcca ccatgtacta aaactatatt gattccaaca taactcatta
     6841 acacaaggtc ttccataaca gattctttta gaccatcttt tttcattgca cttccaccat
     6901 acttaactac aatagtcttt ccctgatgtt tttctatata tggtaaagct tctataagtg
     6961 tatttgcttt ttctatatta atcattaaag tatatcctcc taatttctat aacttccatt
     7021 tattcttaca taatcatagc ttaaatcaca tccccatgca tttcctctgg cattgcccat
     7081 atttaaatct actaatatac ttatttcgtc atcttcaagg attttttttg ctttttcctc
     7141 attaaaatct aatccatttc catttttaca tacttcaata tatcctttca tactctcaaa
     7201 aataatatca actttttcca tatctatttc ttcacctgca taacctaaag cacacagtat
     7261 tctaccccaa tttgcgtcag ctccaaatac agctgtcttt actaatgaag aagatataac
     7321 tgtttttgct aattttactg catgtgcttc acttaatgct ccatttatat agcattcaat
     7381 tagttttgta catccttctc catcttttgc aacaagttta gcaagttcta tacagacata
     7441 tgtaagtgca ctcaaaaata cttgataatg ttcatctttt ttgtttattc tatcattttt
     7501 tgcttttcca tttgctagaa tcattaccat atcattagta cttgtatctc catctacact
     7561 aactctatta aaacttttgt caacagcaat ttttaaagct tcttttagca aaactccatc
     7621 aatatttgca tctgtagtta taaatcctaa cgttgttgcc atattaggat gtatcatacc
     7681 agaacctttt gccatacctc caatagttat ttttttatct ccatacatta ttgctactgc
     7741 tatttctttt tttataatat cagttgtcat aatagcttct cttgctccaa tatgaccttg
     7801 tttacttaga ttattcacta attcatccat attttcttct attaaatcta tatttaatgg
     7861 ctttcctata acacctgtag atgcaaccaa aacatcactt tctttaatat ttaatttatc
     7921 actggtgaat ttagccattt tataagcatt tataagtcct tcttttccat taaaggtatt
     7981 agcattagca ctatttacta tgatagcttg tgcttttcca tttttaatgt gttcctgtgt
     8041 aacataaaca ggattgccct ttactttatt ttgagttact actgctgctg catcacataa
     8101 aacatctgaa taaaccaaag ctaaatctct tttttcttta ttttttctca agccacagtg
     8161 aatccctgaa gcaaaaaatc cttctgaaac tgtaactcca ccctttataa tctccatatt
     8221 tatcctccta attatatgag tttagtattt tttatttcta aaatgatggt gcaatatttt
     8281 ttaaacctgt attttcttca ataccaaata ttatattcat gttttgtata gcttgacctg
     8341 ctgctccctt aatcatatta tctatagctg aacagattat caatgtatct ccattttcat
     8401 gtaatgatat tacacattta tttgagaatc taacattctt taaagaagct atcttatcta
     8461 atggaagaat ttctataaat tctttaaatt cataataatc agttaatttc ctatgtatat
     8521 catttataga aatattatct tcttttgtac aatatatagt tgataagatt cccctattta
     8581 ctgggattaa gtttggtgta aaaactacac ttactttttc tttgcaggat tcacttaaat
     8641 tttgttctat ttctggagta tgtctgtgtt tccctatttt atatgcagtg atattttcat
     8701 taacctccgt aaagtgacta ctaatcgata attctctacc agcacctgta agacctgatt
     8761 tagaatcaat tattatatta ttatttttta tcaatttaga acttagtaat ggcatcaatg
     8821 gtaaagaaat actagttgga taacatccag gatttgcaat tatatctgca tcttttatat
     8881 cttctttgta tatttcactt aatccatata cagctttttt atgtaagatt ttatctataa
     8941 aagatactcc ataccattta ctatatactt cttcatcttt tattctaaag tcagcaccta
     9001 aatctataac tttctttttc gatttaattg cctttattac aaacttttca ctaactccat
     9061 gtggaagggc tgtaaacaca acatcacaca tatctataac tttttcattt tctatacata
     9121 tcatgttatt tttatatcct atacttggat atatatcaac tatatctttt ccaacatacg
     9181 aatttgaacc tatcgctgtc acttcgactt tatcatgatt cattaataat ctaatcaact
     9241 ctgccccgac atatccagtt gcaccaataa taccaacttt tattttattc attttgttcc
     9301 ctccattttt atattcaaat agtcttaaaa tcctatcttt ccactttact atatgataat
     9361 catttgataa tacgtagatt aaaatactct aattaaaaca aaaaacccct tagcattata
     9421 aatactaaga ggcgattttc cgcggtacca ctcttattgg taaaattaca ataatcatta
     9481 aaactcaaat taaaaagtat ctacaaaatg tacatttaaa attaaaaaag cctcttggtc
     9541 aaagaccaag agacgaatat acacccgcgt taccactcta attgataaga taatcttacc
     9601 cactctaatc tcttaaggcg agattcacca attaccatac tattacttct ggtaatctcc
     9661 tccaaagctc tattcacaat aaaattcgtt actaggcttc caccattccc agctctctat
     9721 caactagctt tatgctactc tctttttcac aggatttcaa tttattttct tattgtttta
     9781 tatgatagtt tattttttta actttgtcaa tataattttt aaatttttgg aaatattttc
     9841 tatatggtta atttccagca ttttgtatct aaattgcgat ataattttta tatttcattc
     9901 gtcaatttaa aaaacatcca aaatatatta aattataaaa taatatattt tcaaaataca
     9961 atatttacat ctctttttaa ttaaattttt taatgttggt atctaaaaat ttctcaatct
    10021 ttttctcaga aagtatctcg ttggttacaa attcaccatt aaaaaaaaga ctgtatgtag
    10081 taaatggtgc aggagcattt tgtgcttctt ttgctgtttc gaattttata agatttacat
    10141 caatacctct agaatttgca atttttttca aaataggagc atatttttcc gtatgtggac
    10201 actgattact gtaatataaa actataccat tctcatttaa ttttctttgt tttacacaat
    10261 tcttaaaaca tggaatagga gcattatcag taaagggaaa atataatagc tcataatttg
    10321 gttctgatgt atctgctaaa caaaagtcct tatattttag atatttagca tcagataaaa
    10381 aaggcatttt tttactagac gaaagcacaa ctaatccata tttacctttt gattttgcat
    10441 catcaacaca ctcttgtagc aatagattag aatatccttg acccttaaat tttccagata
    10501 tccaaaaaca gttaataaat atataattgt ccgcagtaat aggacaccat gcattttctg
    10561 ctggaatata ttctataaaa gctttacctc ttacatcaaa tcgtttaaag actagacctt
    10621 tatcaaaatt atttttcatc caagcttttt tagaaaaaac acaattttct cctttttttt
    10681 cagaaattga acaacatata tgttcattgt caatattatt tatatttata tcaataatct
    10741 gcatacttat ctccttttct aattgcataa taatttttaa atatattcta tatattttta
    10801 tcttaatatt ttgaatacaa caacatcatg tcatatatat ttttatttta cataatatta
    10861 ttttaagatt atctgaatta ttaaattgta tatataatta aatacattct atggtctaac
    10921 tacactataa tataaattat atattatcat aatattctaa atttataaaa caaattgtat
    10981 tgaattgaat ttatttaaga tatataatta taattaaaat aataataaag gtgttgttta
    11041 taatgttcaa agaatactta ataagagaat taaaagaaga tgatattaaa aatgcattag
    11101 acttaatctg gaacgtattt gaagaatttg aatctccaga ttattgtgat gaaggaatta
    11161 cgacatttca tcaatttata gaatataact ctattattga aaaaatcaaa gaaaaaactc
    11221 tcttcttttg gggttgtttt gactctaaaa aacttattgg agtcttagct attagaaatt
    11281 taaatcatat atgtatgtta tttgtagata aaaactatca taaacatggt gttgcaaaaa
    11341 aattaatgac tacaggcata caatactgta tttgtaataa atttaattta actgaaataa
    11401 ctgtaaattc atcaccttat gctgttgaag tttatcataa attaggattt aaagatactg
    11461 ctgttgagca aattgttgac ggtatacgtt ttacacctat gaaattgtta atataataaa
    11521 ttaaataaaa agcaaagtct cttaaatatt ttatagttac tttgctttta aaccaagatt
    11581 taaaataata ctatcttttt tgttttttaa taatatactt taaaatcctt actaatatga
    11641 actataattt attttttgat atcctttttg attaacatat aaattaattc aattattaaa
    11701 cataaactat gttgtttctc tttatatctc ttctttgtaa acaatattgg gcatttgaaa
    11761 ataaatattc ttaaaatata ctgttctcct gttgattttc ctttggtaca aatgataaaa
    11821 atttaattta catgccccat aatgaagtgt tcaataaata agttaaaata atttatttac
    11881 ctccttaatc atatttttta tgaataccta ataacacagt atcaatagaa tcgactaaac
    11941 cttttatagc ttcttcttta ctcaaaaatg atttcccaag ttgcaaagtc gaataaccat
    12001 gaagtacact cattattaag tttaatactt cacctgtatt ctttgtattt agattacaag
    12061 aaagaataag tttttctaat aaggatttat attcatcaaa gatttctaat gtttcattat
    12121 ttccatgcca agtagcccac tgaattgttt cataaacacc aggattagaa attataaaat
    12181 taaagtatgc tatacttata cactttatag ctgtatctcc aaaatttcca atagccgcct
    12241 ttatcatttg actattcatc tctctcattc ctttatgagc tacttcaagt aataaatcat
    12301 ctagactttc tatatgattg tacaaagatg gtgtacgtat tcctagcttt tctgcaataa
    12361 cttttaaaga aactttgtta agcccatctt tattaactat atctgatgta gcttgtataa
    12421 ctatttctct actaacacgc atgattttcc ctctttcatt taaaattaat tactttatta
    12481 gtttaactaa tctaattagt ttcgtcaatt attgtaattt ataaaattct ttatactatt
    12541 ataaaatctt gacagtttaa ttataattta atataatact aattaaatta gttttaatac
    12601 taatataatt agtattatat taaattctat attacaacat tattttatag tttgattaat
    12661 ttaataatca ctaatatatc aaactgaaag gagattttta tggaaaaaaa atatttcgaa
    12721 attcatggag ttccttcaat cctttgggga aatccttcaa gtaaattata tatttacata
    12781 catggacaat gtggcaataa agaggaggct gaagcttttg ctaatattgc agttccacat
    12841 gagtgtcaag ttttaagtgt tgatttgcct gaacatggag aacgtaaaag agaaaaaaat
    12901 tcatttaacc catggcatgt tgttccagaa ttagtatcta ttatggaata tgcaaagtgc
    12961 cactggaatc aaatttcgtt atatgcaaat agcattggag catggtttag tatgttaagc
    13021 tttgaaaaag agaacttaag agagtgtatt tttgtatctc caattctaga tatgcaacag
    13081 ctcatttcaa atatgatgct ctgggcaaat gtatcagaag aacagcttaa acatgaacta
    13141 attatcccaa cttcatttgg gcatactctt tcatgggaat atctacaata tgtaaaacaa
    13201 catcctatca ctcaatggaa tataccaaca aaaatattat atggtaaaca tgatgaactt
    13261 actgaaatcg gtgttgtaga gaaatttgta gaaaagttta attgcaacct tactattgta
    13321 gaaaatggca aacattggtt taacacacca caagaattaa attcattata tgaatggtta
    13381 aatactttag ttaaataaaa aataatt
//
LOCUS       DAMJWU010000100        13020 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMJWU010000100 DAMJWU010000000
VERSION     DAMJWU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.104"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            96..977
                     /locus_tag="PC460_002993"
     CDS             96..977
                     /locus_tag="PC460_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2601864.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1185..2048)
                     /locus_tag="PC460_002994"
     CDS             complement(1185..2048)
                     /locus_tag="PC460_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF2601865.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2203..4137)
                     /locus_tag="PC460_002995"
     CDS             complement(2203..4137)
                     /locus_tag="PC460_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF2601866.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4249..5262)
                     /gene="galE"
                     /locus_tag="PC460_002996"
     CDS             complement(4249..5262)
                     /gene="galE"
                     /locus_tag="PC460_002996"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF2601867.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5286..6155)
                     /gene="galU"
                     /locus_tag="PC460_002997"
     CDS             complement(5286..6155)
                     /gene="galU"
                     /locus_tag="PC460_002997"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2601868.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6353..6673)
                     /locus_tag="PC460_002998"
     CDS             complement(6353..6673)
                     /locus_tag="PC460_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601869.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6663..8654)
                     /locus_tag="PC460_002999"
     CDS             complement(6663..8654)
                     /locus_tag="PC460_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601870.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8777..11320)
                     /locus_tag="PC460_003000"
     CDS             complement(8777..11320)
                     /locus_tag="PC460_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF2601871.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11598..11932)
                     /gene="rnpB"
                     /locus_tag="PC460_003001"
     ncRNA           complement(11598..11932)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC460_003001"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12064..12741
                     /gene="srtB"
                     /locus_tag="PC460_003002"
     CDS             12064..12741
                     /gene="srtB"
                     /locus_tag="PC460_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF2601872.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12901..12975)
                     /locus_tag="PC460_003003"
     tRNA            complement(12901..12975)
                     /locus_tag="PC460_003003"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12941..12943),aa:Thr,seq:tgt)
BASE COUNT         4007 a         2288 c         1330 g         5395 t
ORIGIN      
        1 ctctataatc atatataata aaaatgtaaa aatatcttaa aggttaactt ctacaaatat
       61 ataaataaaa atacctatga aaggatgaac aaaaattgaa tttatatcac ttacgctatt
      121 ttgttacatt ggcacattta gaacactata caaaagcggc agaaaattta tctataacac
      181 aaccaagctt aagtcatgct atttctttac tagaaaatga acttggggtg gctctatttg
      241 aaaaagaagg tagaaatata gtccttacta agtatggaaa aatattttta aaagatgtgg
      301 aaaaatcatt agaaatttta gattcaagtg taaaatcatt aaaaataact ggtactggtg
      361 aaggacaaat agacttagct tttcttagaa cacttggtac tgattttatc ccagacatag
      421 tgcacaaatt tttaaaatct aatcctgcta aatcaataga ttttaaattt catactggtg
      481 taactactga cattattcaa ggtcttaaag aaagaaaata tgatattgca ttttgttcga
      541 aacttgaaaa agaaaaggga attgaattta tacctgttgc caaacaagat ttagtactaa
      601 ttgtaccata tagtcatcca ctagctgcaa aggatactat agatttaaaa gaaaccatac
      661 cttatcctca aatagtattt aatcagagaa gtggtcttag atatataatt gatgatatgt
      721 ttaaaaaaat aaatcaacag cctaatatag tctatgaagt agaggaagac caagtaattg
      781 ctggattagt tgcaaaaaat ttcggaattg ctgtagtccc aaatatgaat atgcttagct
      841 ttacaaaagt aaaagtaatt cagattattc acccaagttg ggaaagaaac ttttacttag
      901 catttataaa agatagatat ttgcctcctg caattaaaaa ctttaaaaac tttgtcatta
      961 aaaacgctca attataattt attaataaag gggttatttt taaatatcct cttttattct
     1021 ttttataaaa taaaaaaatc tatactttta aaagtataga ttcctaactg acagtcggca
     1081 tccgagtctc agtaattatc gttatacaac agcaactgga ttcagttcct gaataactag
     1141 gttacttaca ttatatcaga ttttttaaaa tttgtcaatt gtatctaacg cattttttca
     1201 agccatggtc caacaggctc tacaatagga acttctttaa atccccaatt tcttatatca
     1261 ttcacactcg ttggagtata tattttgttt ctgacagatt tgaatatttt gtttttttct
     1321 ccatattgca tacactttgg attagcttct attaaccctc ttcttgctct gctaagagga
     1381 ttacataaaa tatctgctat ttctaaacct tctctatagt cagcatcttc ttttttggca
     1441 attataaaac ccttaacctt atctcttaat tcttcatcta tatctaagta tattgttcca
     1501 ttattaaata catcaaaaaa cgccttttgt acttttaagt ttgagacatc atcccttgac
     1561 tctataacta ttcgtgcaga ttctacagtt tcattattta caaatgaata aaaattctga
     1621 agaacatgta taaaagccac aacatacggt tcctttgaag gtgcataata tttctttaat
     1681 ctagctttat ctactgttac tgatattata gtacactcta atctagatag aaataaaggc
     1741 aactcatcat agaataactt aatctgtgaa tcatcaaact tctcaaatgc tcttgatttt
     1801 cttgatatat ctgtcaaatg tattcctatg ttactggttc caaaacaata ctctttaaat
     1861 gcatccattt ctttcctcaa agaacataca taatcgcctt gacttactgc atatttatat
     1921 tcaaaaatta ctcctgtcaa tgtaaatggg tcttcatgat ttatatcagc tcttccactt
     1981 tcatcaataa acattatata ttttttcttt ataatcttac tcaccctctt aacatatgat
     2041 gatagcatct atattcattt tatatgagtt ttaaaaatat tccaagattt ttatataact
     2101 taaacttaat tttatactaa aaaattgaga tgtaaaacaa tcaagctttt attgattgaa
     2161 tttcacatct caattataat tatttttcaa tatatatttt tactattttg caagaatatc
     2221 cattaattta tccattacta tataagatac tccgcctcct atttgataag gagtttcaac
     2281 ttttttattt gcaaatactc tttgttgctc tgatgataat cctgaattat ccattaaaac
     2341 tattggtgaa tgtaatttta cagctaatgg tccagctgat aaagcatcta ctaaagcatc
     2401 tgatttacta actaatacag cctgtaaatc agtatctcca tagaattttt ctatcacttt
     2461 ggcattagta tctactctac tatctccata tattctattt cctgaaatat cttcagtagt
     2521 tatatcattc atttttgcta ttactgaatc gttaattgca cttggtccac ctataaagta
     2581 tgcattttct aattttcttt ctgttagcca tttataagat tcttctgtaa ttccatcttt
     2641 attgactaat attataggtt gtttttcttc cccagcttta gaagctacag ataaagcatc
     2701 tgcctcccca tttccagaaa ccatatatgc agtttttatt tcttctcgat ttccaagttc
     2761 ttcagctatt cttgtagcag tttgatatct atcttgacct gctattctct ttgtttctgt
     2821 gttgaaagta gtttttattt cttgctctaa tttagaactt attgcatttt ctccacctat
     2881 caagattact gttttaggtg ctaataactt catttcttca acagtttcag ttggtaaatt
     2941 agctgtatta gtaagtagta ttgttgagtc atagctactt gctaaaggag ttgctgttat
     3001 accatcagct attgcattac catttactaa tattacagta tctgaactat cagcattcca
     3061 gtttccaact gaacttatct ttttagctgt atcaaatcta tttgcaccta taattgtatc
     3121 ccatgtagga tttatagttc catcttcatt aaaagtataa tctttcccat ctattttttg
     3181 agaaccagtt agcatctttc catctttgtc taaatagtaa gacttaccat ctatttgttg
     3241 ccatcctgtc tgcattattc ctgttttgtc aaagtaatac ttattaccat ctatttcttg
     3301 ccatcctgtt actgctaaac catcagatcc tagatagtat ttctttccat tcttttcttg
     3361 ccatcctgtt tgcattactc catctgagtt aaagtaataa gtttttccgt ctattacttt
     3421 ccatccagat actcttgctc catcagttcc taaataatat tttttgttat taattgtttt
     3481 ccatcctgtt tgcattactc ctgaagcatc aaagtaatac atttttccat ttacttttag
     3541 ccaactattc ttttggtatg ttccatcaga ttttacataa tacttcttac cattttctgt
     3601 tttccatcct gtttgattta gatttatctt atattttgcc gctatcttta catagctgtc
     3661 gttactataa tctataatta catctgaacc tacaggaact tggtcaaata accatctaac
     3721 atctttattg tgcattctta tacatccacc agaaatatgc tttcctatag aactctcatt
     3781 attatttcca tgtattccat atgtatctcc ataagttcct tttaatgcta atcccatcca
     3841 tctatctcct aatggatttc ttggacttcc tccaggtata ttacctttat aatatggtct
     3901 attttttatc ttgttaacta cttttgtttt ccctgttggt gtttctgacc cctttttacc
     3961 tgttgcaact ctaaattctt ttacaagttt attatttaca aaatatccta gagtattttt
     4021 tcttgaattt actataatca catgtttact ggctgcgctt gattctggta cgccacatgc
     4081 aaatactaaa actaaagtca gtgctagtac taaatacttt tttagacttt ttttcactat
     4141 aacttccccc tcttttagaa acattactac atgtgtctaa ttataacata aaattaccaa
     4201 taccttatat ttacaaattt gttacattta tacatataag tatgtattct actcttcata
     4261 cccattagga ttcatacttt gccatctcca tgaatcttca cacatctctt caagttcata
     4321 cttagctttc catccaagct ctttttctgc ctttgaagaa tctgcataac acatggctac
     4381 atctccagcc cttcttccta caatcttata tggtatttct tttccacttg ctttagaaaa
     4441 tgcttttact aaatcaagaa cactataccc ctttccagtt cctaaattat aaacaacaag
     4501 tcctggattt tcttctaatt tttgtaatgc ttttacatga ccagcagcta aatctaatac
     4561 atgtatatag tctctaactc cagttccatc atgtgttgga tagtcattac cataaacact
     4621 caattccttc aatttaccta ctgctatttt tgttatataa ggcattagat tgctcggaac
     4681 tccatttggt tcttcaccta ttctaccact tttatgagca ccgactggat taaagtatct
     4741 taaaagagct atatccaatg acttatctgc ttttgatata tctactaaca tctgctctat
     4801 cataagttta gttcttccat atggattggt tacagaaagt ggaaaatcct ctaatatagg
     4861 acaagtatgt gggtctccat atacagtagc tgaagaacta aatacaaact ttttaacacc
     4921 atattctctc attaattcaa ataaatttaa ggtacttatt agattattgc tgtaatattc
     4981 aattggtttt tctactgatt caccaactgc ttttaaagct gcaaagtgta taattgattc
     5041 aatattattt tctttaaata caacatgcat ttcatcttta tttctgatat caatattata
     5101 aaattttact ggttttttag ataattcctt gattctatca accactattg agttgctatt
     5161 acttaagtta tcaactataa caacttcata acctgattct aaaagttcta ttgctgtatg
     5221 gcttcctata tatcctgctc ctccagctac taaaactgcc attgtaatta cctcttttct
     5281 aatatttatt tttcacttac tttttttctc atatagtcca ttaaatcatc ttttaaattt
     5341 tctctcttta gagcaaagtc tattgttgcc tctaaaaaac caattttatc tcccacatca
     5401 tatcttctac cttcaaagtt ataagcataa atagcctctt gttttccaag agtttgaagt
     5461 gcatctgtca gttgtatctc tccaccttta ccaggtgtct gtttctctaa tatgctaaat
     5521 attgcaggag ttattatata tcttccaagt atagctatat cagatggagc ttcttcaatt
     5581 gcaggttttt caaccatatc tttaacttta tatactctat cttcgatatg ttttacatcc
     5641 aatataccat atttattagt atcttcttta gctatctttt gaacacctaa tacagttgtt
     5701 ttatactcat tataagtatc tattaattgt tttaagcacg gaacatctga atctactata
     5761 tcatcaccta ataatacggc aaaaggttca tcacctataa aactttttgc acagtaaatt
     5821 gcatgtccaa gaccttttgg ctctttttgt cttatataat gaatatttac catatttgat
     5881 atgtctctta ccatttctaa catctctgtc ttaccttttt gctctagttc taactctaat
     5941 tcaactgatt tatcaaaatg atcttctata gattttttat ttctacctgt tattataaga
     6001 atttcttcaa ttcccgattc tatagcttct tctattatat actgtaaagt tggtttatct
     6061 actataggaa gcatttcttt gggctgtgat tttgttgctg gtaaaaatct agttcctagc
     6121 cctgctgctg gtattattgc ttttttaact gtcatctgca ttttttcacc cttcttcgta
     6181 aattttctat tgtaccaaat taatatacca tatttatact ttttcttcaa actctatatt
     6241 atcccaatat ttaatgctat aagataatat aaggtagatg aaatttcatc tacctttagt
     6301 tttataagtt aatattgtat tgtttatatt ttatccaaaa cttatacagg tactaaattt
     6361 catctaagat gccttttata tcactatcta tatcttcatc tagataagaa tctaaatctt
     6421 ctaagtcaac ttctagttct tgatatgtca tatcaacact gacatcctca tctatatcca
     6481 tttctgatat taattctcct aaaatactat ttccatcaga atctaaacca ctatctatat
     6541 cttcatctat atagttttca tttaaaattt catctaaatc aatctcgtaa taaacttcat
     6601 tatttttttc atcccctgag tccaatccta agaactcata gttaaaagtc caaacattgt
     6661 atttacttga catactttat tcctcctgtg aaaaacatat tttcatctag tttattggtt
     6721 ccataatcaa cagagttata tattttttca attatgtctt ttcttctttc ttttgtacta
     6781 ttatttaatg tcaattgttt aaatacatat gtatttgtta ttctctcgaa taatatttct
     6841 tttatactat catcatcaat atttctatag tttttccaca ataactcaaa taacttaaac
     6901 atttccctac tagtttcatc tacataataa ggatacatat tttctactaa atggtttaat
     6961 cttgatatgg cttgttttaa aaatacatca agacttgcac tttcactttc ttttaacatt
     7021 ctataattgt atttcttctt atcttctcct gaaattacaa agatgatatt gtctaaactt
     7081 ttttcaactt catttaaata actgcttata ttatatgata aatcagacaa tagattttta
     7141 atctgcttaa acacttgtac atcaagttta gtattatact tttcaaacaa atctatttct
     7201 tgattttcta atattcttgg catatatgct gacaataaat ctaaattgtt tctttgttct
     7261 tgtatttgct tataaagtga atttataact tctattctta tttcatcatt taactctttt
     7321 tctaaaatct tatcaaattc agatttcata tagtaaatat ttccaaagaa attaaatatt
     7381 atcatatcat tcatagcttt agaccaattc ttatatatca cttcaataga attttcttta
     7441 agtagaggtt gctttgcata tataaataca tcttgcataa gtttttgaac catactttta
     7501 cttaagttta aaagtacttt ttgattatta aattttattt ccgtttctac aaaattagaa
     7561 ttttcgtttt caagttcttt attaattaca atgtctttta tatcactatt ttttatagat
     7621 atgatttctt tttgaaaaga ctttagaata gtcaagctat cgaaatcttc ttgaataaaa
     7681 aatggagcat gttttaatgt atatcccact atttcatttt ctatatcttt taggtcatct
     7741 atttgcataa tttctctatt tttatcattt atatgtaggt taaatacatt tttattatca
     7801 tataatatta tatcttctcc taaatttaac ttagaacaat tatctttcgc tgactttaca
     7861 atagtcttct tagatttctt atttataaaa attaattggt cttgatattt tactaaaagc
     7921 atgtttgtgt ttttataatc aaatccatct atatttcctg atatcttgca gaatactatt
     7981 tcacaatctt taggaagatt tcctattttt tggttatctt taactctctc atttaaatca
     8041 gatgggacta cactatctat attgttatta aatatagata cagaccttac tataatcccc
     8101 tctctttcca tatgaaagta tcccttcaat gacactcttt tatcttctaa aatgtatctt
     8161 tctatatcat caaaatttat ttcaaatcta tttttcccac tgataaatat aaacttctca
     8221 ttatctttat taattccacc catataaaac ttattgtcat ctaaacttaa tatagtcata
     8281 ttatccttta gatagaatat aaattctctt tgacttttgc agattaaatc atattcatcc
     8341 aaagttattt tcatagtcaa atttgctgta acatttttac ttgaattttc actttttatg
     8401 taaaaaggca agttatttcc atagacaatt atttgagaat cttctaaaac tgcctctaat
     8461 ttttctttat tgtctgaaaa actatatata tattttacac ctgtatcctc attaacttca
     8521 tctgatttgc tgattattat attccctaga tttgttatat gaagcttttt atattctttt
     8581 gagtatctac ttatgccact aagctcaaac ccatctgaaa gctcatatat gattttttct
     8641 cctaatttca acataccttc accacctaaa cagaattata agtattttat aaaggtgaat
     8701 aaaatgtctc aaaacaaata atattttttt taatactttt tattctgaga catagtttct
     8761 ccttttttat aatgctctaa gcaccttcaa ctatttcttc ataaagttta tctaaatgtt
     8821 taacatattc acgatatcca gatttgttct ttaactcact tcttaaatct tcatattttt
     8881 gtttaagttc ttcagcactc aaagaaggag ctgattcttt taattcttct aacttataat
     8941 catagtactt atcatacata gctatatatg tatcaataga cttcttagct agttcattac
     9001 atttttcttg tgcttctcca tattgagaat aatcacctat ttttatttta tagtatccat
     9061 tttcttgaat tattgttgaa gaaatacctt tagcttcata ttcttttgct gtttcttttg
     9121 ctgttttttc aactttaagt gaacctgcat aaacagtgta atatacacct ttaccatctt
     9181 tatatgcttc catttctttt gctttctttt cagcttgtgc tttttcttga gcctcttttt
     9241 cagcttttgc cttttcttct gcttcttttt tctctttttc agcttgtgct tttttctctt
     9301 cttcagttaa tttattgtta gcttgttgtt gagttgatga ctttggaata ttctctttat
     9361 catttttctt attgttgcct actgttataa aatatcctac accaacaata accactataa
     9421 ctactgctat aatagtttct tttaatctac tcttcttaga aacaacctct tcatcatcta
     9481 aagaatcttc tttatctgat acataatttt tcatttcttt cttgttttct ctatatttgt
     9541 ccttagaaga atttttagat tttttattac tattttcttt ctttttaata ggtattacta
     9601 ctgatttttc attttctaat ttttcttctt ttatttcttt tgctttttta tcttctaagt
     9661 ttttactttc tttttcaagc ttcgattcat tatttgaaga tactttttca tctaagttat
     9721 tatttattgg tttttctatt tttacactac gttcttcttc tatatgttga cttggttctt
     9781 cttgttttat ttctttcaat tcattttgct ctatttcttc tgatttcact tgatgatttt
     9841 caactggttt atctaaaatt atatctggag aaatatcatt taaattctct tcttcttgtt
     9901 cagcttcttc attgtctttt ttaataaatc caaataattt agccttcttt ttactctttt
     9961 tattttctgg ttcaattata tcttctttat tgctgtcgta ctctttatct gtagtatctt
    10021 tatcttctac aacgtcacct atatcttgat tattactatc tacttcttct ttagtccctt
    10081 cttcagcatc atcttctatt gaaatactac ttctaaagca aatatcttta tctgcatttt
    10141 ttatcatgta aatgtattct tcaatatcga tatctttatt tcttctagtt ctttttgatg
    10201 caatttcatg attttctaat tcactatagt tatcttttat tagattctgt gctttattcc
    10261 atgtatcttc actaatttct gttttatttt taattacata gtccacaaat tccttaaaat
    10321 tttcaggacc aaattctcca attatagtca gtctcattgc accttctata aggtctctat
    10381 ttcttctctt taaaaattct aatacaactg ttgcacaagc aaaatcttta ataatttctt
    10441 tgccaaaacc atatgcttca acagaatgaa gttcttctaa tccacataac ttcttatatt
    10501 cattgtgtat tgtttgagat atactttcat tatttaagct atttaactta tctataaact
    10561 tcgattctat atcatttgat tttgattcat gttttacttt tgaattttta atttcattta
    10621 gtagcatgtc attttctgaa ttatctttac ctaattgcaa atcctcaatt tcagttgctt
    10681 ttatttctac tttgttttgt tcgttattct cttccacatt attaggtacc acatctttat
    10741 tactagctaa tattttgttt acacagaata taatttctct ttttaattct cttttcgcat
    10801 ctgcttgatt tttaagaaat aaatctccac attctttctt ttctaaactt atttttctaa
    10861 gagatatatt aagaaggtct ttatataggt ttaaaacctt ataatccttt tctaaacgtc
    10921 ttcttctctc tgatagttcc attttatcat aagctaaaaa actatttaaa tcattggctt
    10981 ttagagagtc taatccctct ttcatagcct tatttacata ctttctacat tttgctgtag
    11041 ttaactctaa atctttttct aattcatcta tttcaggtat gtacttatct actatccatt
    11101 tgccaataag tggttctgta tttcttgcta atgtcttata aaacaatata ttaaatttta
    11161 aaagctctgc atattttgct tcattaaatt ctactttttc aaatttcttt tccaaaacac
    11221 catctagctt ttcatttatt ttagactctt gttttaaaaa acttgctgtg acatttaaaa
    11281 cagtgtttct tattatatta ttttcactca aaaccttcac tccttaaatc aattatttta
    11341 ttgtaatatt tacattgtgc aaagttgcta atttgattat gatatctaaa taattattta
    11401 ctaaatttgc tacatcttat atcatatcat attatttaca tcattttact ttttcttttt
    11461 atatctatct tatattatac catttttaat atctgtacta acttatatta caatattcct
    11521 caaaatttta aaagtagatt agacaaattt acaaataaaa aaacatcagt ttaaactgat
    11581 gtttttgact atcgaaataa ttaagcaagt ctataagcgg agttctgtat tagataatca
    11641 tctttctagg cctattgtca ccaataggct caagcgacct acctaccgga gagtacgagc
    11701 agcactcttt attcctatct tggtcttgct ccaggtgggg tttacatagc caactagtcg
    11761 cctagctgct ggtgagctct tacctcgcct ttccatcctt accacaatag tggcggttta
    11821 tttctgttgc actatcctta ggatcgctcc cactaggcgt tacctagcac cctgctctat
    11881 ggagctccga ctttcctcat gtaagattac atgcgattat ctggcttact taatatttat
    11941 tttacttagt tatgttaaca caaattcttt tacatgtcaa agtaatatta tactaaaaat
    12001 ttaaatttat tcaaaaatag tgtaatatag atatataaac tacatttaac tcgaggtgat
    12061 ctattgaaaa aattatatag aatagtaatt aacattatac tagtattagt aatactttac
    12121 agtggattta atatatactc aaaacttacc aaatacaatc atgatactaa aatatcctca
    12181 gaacttcaaa aaaaagaata taaaaaagag gatttatcaa agattaacag tgattttaaa
    12241 ttttggcttt ctgttgagaa tacaaatata aattatccag ttgtacaatc taaagataat
    12301 tcttactatc ttgacaaaga tttttataaa aaagattcta tttctggtac actgtttatg
    12361 gattatagaa ataaatctat tgatgacaaa aatatcataa tatatggaca taatatgaaa
    12421 aataaaacta tgttcaacaa cctaaataag tttaaagatg ctgacttctt taaaaaaaat
    12481 aataaaataa aaataacctt gaatggaaaa gaatttttat atgatgtttt ttctgcttat
    12541 attgtagaat ctgattatga ttatctaaaa acaaatttta acaatgaatc tgattatcaa
    12601 aattacataa atgatattac ttctaaatca ttgtataaat ctcctattaa ggtaaatagc
    12661 aatgataaaa tagttacact ttctacatgt acctatgaat ttgatgatgc cagaatggtg
    12721 attcatggta gattgattta aaactcacag acacagttta aaacaataat tttaaactgt
    12781 gtcatctttt atacctataa tttgtaaatt agaccactaa aagtatttaa tacaaaataa
    12841 caaatacatc aaacaataaa aaacaataaa aaaagactat gctaaaaaca tagtctttac
    12901 tggagctggt gataggaatc gaacctacaa cctgctgatt acaagtcagc tgctctaccg
    12961 ttgagccaca ccagcacacc tattttattg taaattagca aaactaattt ttaaaagtta
//
LOCUS       DAMJWU010000101        12831 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMJWU010000101 DAMJWU010000000
VERSION     DAMJWU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.105"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            131..319
                     /locus_tag="PC460_003004"
     CDS             131..319
                     /locus_tag="PC460_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF2601873.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(479..631)
                     /locus_tag="PC460_003005"
     CDS             complement(479..631)
                     /locus_tag="PC460_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF2601874.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(729..1205)
                     /locus_tag="PC460_003006"
     CDS             complement(729..1205)
                     /locus_tag="PC460_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2601875.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1424..2185
                     /locus_tag="PC460_003007"
     CDS             1424..2185
                     /locus_tag="PC460_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2601876.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2445..4373)
                     /locus_tag="PC460_003008"
     CDS             complement(2445..4373)
                     /locus_tag="PC460_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2601877.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4607..5059
                     /locus_tag="PC460_003009"
     CDS             4607..5059
                     /locus_tag="PC460_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2601878.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTNEVKNASDASHLVKYITNKMKEE"
     gene            5062..5370
                     /locus_tag="PC460_003010"
     CDS             5062..5370
                     /locus_tag="PC460_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2601879.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5484..6590
                     /locus_tag="PC460_003011"
     CDS             5484..6590
                     /locus_tag="PC460_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2601880.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6665..9343
                     /gene="mngB"
                     /locus_tag="PC460_003012"
     CDS             6665..9343
                     /gene="mngB"
                     /locus_tag="PC460_003012"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2601881.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9660..11993)
                     /locus_tag="PC460_003013"
     CDS             complement(9660..11993)
                     /locus_tag="PC460_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2601882.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11968..12684)
                     /locus_tag="PC460_003014"
     CDS             complement(11968..12684)
                     /locus_tag="PC460_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2601883.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4786 a         1792 c         1669 g         4584 t
ORIGIN      
        1 tatgataata agaaattctt caatatgtgt aaggcaaata tatatttcta ttgcaaattt
       61 ttgttattta tgataatata aatatgtttt tagtctttaa atagttatca ttttaaggag
      121 gtgtgcgtag atggcaaaag tatgtagcgt atgtggtaaa ggtaaggttt ctggaaacca
      181 agtatcacac tcaaataaac ataataaaag aacatggtca gctaacttaa gaagcgtaag
      241 agcgattata gatggagctc ctaagagagt aaaagtttgt actagatgtt tacgttctgg
      301 taaaattgaa agagcttagt aaccacgttt aaaagcctac ttccgtaagt aggcttttta
      361 atttaatcat ttttactctt tctcataaat tttaggatta tacttgaaat ccattttggt
      421 aattttattg ttactacttt catcaatact caccccacac tttatagtta aaactatttt
      481 aacaaaaaaa taataatgct ccacatccaa taagtattaa tgcggtaagt gctgaccaaa
      541 tccagccagg aagcaacatt gacagaatta cagccattcc tgttgctaga caaagtattc
      601 ctagcaatac cttagacttg ttatttctca tggaacacct ccacatagaa cctgtatcta
      661 taaatatttt catattcaag tttatgcgac agttttccat tttagaccaa taaacacatt
      721 atgtaatttt aatttttcat actagaatta ttactgaact ccgatataga ttctacaata
      781 tccaaaattc ctttttttct acttaacctt tcaggtccac tatataaaac aactggtata
      841 actttaggaa gtttaaaatt tttatcttct atttcttcaa ggcttttttt cataataaaa
      901 cttttccaaa tttgttctct atacgatttc aatcttggga caatttcagt atccatttct
      961 gtttgaaatt ctaacactat ataatgtgat actttgccac ttttagaaaa taccttataa
     1021 attatatcaa cttcttttcc tttatattca catataaaag atgtttctaa ctctatattt
     1081 ttaagagtta attcttttgc tattgagaca cttgtaaaat tttgaatcaa acttaaaaaa
     1141 ctttctttat ctgaatacat tcttctgtac tcttctcgtt tttcattgct tctttttaaa
     1201 ttcatacagt tcctcctaga ctatagaccc tctatttttt agtaaagata gtctataaat
     1261 tttatttttt aattttcatt agtgagtatt gacagttgtg tgtaaaatat ccagtaaaga
     1321 tattttttta tatttttatt aaaagatata aatttttata ttttaatgta ttgacattta
     1381 cgttgcgtaa aagtgtatat ttaatttata ggaggtacta cttatggaat atacagttca
     1441 aaaattaagt aaactagctg gtataagtac tagaacactt agatattatg atgaaatagg
     1501 tcttcttaaa ccacttaaaa taaattcttc aggttatcgt atatatggtc aaaatgaagt
     1561 taataaactt caacaaatac ttttttatag agaattagga attaacttag aaaatataaa
     1621 aaacattata aattcaccaa acttcgatag tttaagtgcg ttaaaagaac accataataa
     1681 attactagcc aaaagaaagc aaattgattt actaatagaa aatgtaacta aaactatagc
     1741 tctgaaggag ggaaaataca ctatgacaga tatagaaaaa tttgaaggtt ttaaagaaaa
     1801 gatgattgat gaaaatgaaa aaaattatgg aactgaaatt agagagaaat atggtaaaga
     1861 tattatagat acatcaaata aaaaacttaa aaatatgtct aagcaagatt atgaaaattg
     1921 gcaaaaccta aatatcgaaa taattagtaa acttaaaaaa gcttttaaaa ctggtgatgc
     1981 ctctagtgaa ctggctcagg aagttgcaaa attacatcat aaatggttga gttacacttg
     2041 gaacacatac tctaaagaag ctcattcctc tctagcacag atgtatgtat atgatgaacg
     2101 ttttacatca tattatgata gagaacaacc tggattagct aagtttttaa gggatgctat
     2161 agtaatatat acaagagata aataatataa aataataaag gtggtgtctc aaaatgaact
     2221 ttttagttca tgagacactc ttttttgtat gaaatcagat atatttttat cattttatca
     2281 atgaaaaaca ggtttctctc tctattttga gacagaatct ttattgtata ataattataa
     2341 tcaatacaaa ataataacaa attatacaaa acttacttat actatttatg ctaaaaacaa
     2401 aaaatatttt attaaagttt ttaaaactta tgtaatataa aaatctatac tatagacttg
     2461 attatggaaa gaaaattatc aaaatcatta caattagata tactatctct aatatcattg
     2521 tcatgaatta tctttaaaag gatatcataa atggagttta tttttatata atcatcttta
     2581 tttatagcca acatcatgat taatttaaca gtagaatctt cactccaata tattggttca
     2641 tccaatatgg aaatacaaat cttagttctt atagagctta attccatagg atgaggtata
     2701 gctaaaaaat catctatatt ggtagaagat aaactttctc tgtaaaatac agattcagca
     2761 aatgatggaa atacaatttc actttgagtt aatttgttgc acatcaactt tataagctca
     2821 tctttatctt tgattttagg attaacaata aataaatttt tatcaaagaa attgtccaaa
     2881 agattactat aaatatggga attattagtg attgacttag atatattctc tatatctttt
     2941 tttaatagtt ttaaatccac taatacaata ggaatactat catgatttaa aggaattgtc
     3001 gatattatta tatctacatc tgacaattca ctagctaaaa attgattaaa tgatagacat
     3061 tgaagtatat ttattttatc atgaaaaact ttgtttaatt gaacctctaa taacctactt
     3121 gaaccatacc ctgaaccaca tactaatact acgtttttac acttaatatt actttgaaaa
     3181 aatctctcta ttccagcacc tatatgcagg gatacatatc caatttcatc ttctgttaaa
     3241 gagtaaatac tatttttaaa aactttttct atagcattta aagttatttc aaatgctaaa
     3301 ggataattac tctttattgt atttataagg ggattcacct tatttaaatt ataagattta
     3361 gaattgagaa ttgatttaaa gtgcagtact aaatcatcaa ataatacact atcatttcta
     3421 aggtcaaaag tatattgatt gttgattact tctaaaattt cttcaacata ctctataatt
     3481 ttagtatcta ccctattgga aacgttatcc aaaagagaac ttttagttat aaaatgatta
     3541 tatagataaa ctctgtctgc ctttgaaata attatagtat atttactctc aatataatta
     3601 aatatatttt caacagttgt attcgattga aaatctgtta aaacaacatc taaaacatta
     3661 tctaagcagt aaccatcaag tattctactt acagtaatag ctaaatggat aataaagttt
     3721 tttagattaa aatctaggaa attgatatta aatttattaa tttcacttaa cactaccctt
     3781 tgaagctcta ttaaatctac attattaaat aattctattt ctctatctgt aattccaatt
     3841 atatagtttt caaaattcct atttatcata ttattagaaa tacatcttcg aatatctatt
     3901 tccttacctt caactttaac tccataatat ggcttagaaa ctaatattaa attatacttt
     3961 gacaaaatag gctttattct ttttatgtca tttagtattg taaatttact cacatacaag
     4021 ctattagcta aatcttctag ttttatatat gtattgttat agagaatata ttttaaaata
     4081 tattgatttc tttctatagg agaatctggt atttcacttg aatccataat atcatcagat
     4141 attaatgcta gatactgttg gtataatgct aaattaagaa tatctatata atatccttct
     4201 ccttttttta ttttaataat agcaccattt ttctctaaag ttccatttat agaagtaata
     4261 tcatttctta tagttctctc actcactttt aaatgattac ttaaagtttt acaagtaatt
     4321 ttatcatcac tttcatttaa taatttaaac aacgtgagca atctcttgtg cataaatatc
     4381 cccccttata gaatttcttc taaattatac tatttacact catataatac aattactcta
     4441 ataaaattgt attataataa tttatttcca cttctatatg gaaattagtt attatcataa
     4501 tataaatttc catgtcgaag tggaaattag ttatatttaa tttaattagg ataatgatta
     4561 caatataaat ataagttaag acattatgaa tggagtggaa aaatatatgg tactacaaga
     4621 aaaatatata tttatagata aagacttaaa aagcaaagaa gaagtattaa attttattgc
     4681 agatagtgct tatatactag gtattacaac taataaagag gctcttttaa aagaccttct
     4741 aaaaagagaa aatgaatttc caacaggaat aaaagatgga tttgcgatac ctcatacaaa
     4801 aagtgtaaat gtaaaggaag cttccatact ctatattaaa acaactaacg agttagagtg
     4861 gaaaacttta gatgatagta aggttagata tgtatttagt ttgttagccc cacataaaaa
     4921 tgaaggaaat atacatttga aaactctatc taaattagcc acatgtttaa tggaggatga
     4981 ttttacaaat gaagttaaaa atgctagtga tgcaagtcat ttagtaaaat atataactaa
     5041 taaaatgaag gaggaataat tatgaaaata gtagcaataa catcatgtat agcaggatta
     5101 gcacacacac ctatggcagc aaaagcttta gaacaagctg catcaaaaat gggtcatgaa
     5161 atcaaagtag aacaacaagg tgctatggga cgagtaaata ctataacaaa agaagaagca
     5221 caagatgcgg actttatact tatagcatct gaccaaacta tatctggaat ggaaagattt
     5281 gatggtaaaa aggtgataaa agtcaaaata ggtatagcgt taaaaaaacc agaagctgtc
     5341 ttaacaaagt gtatagaagc tatttcttaa taaaaaaatc tcactctaat atagaatgag
     5401 aaaaatcaaa aattaaaaaa ttgtctaatg taattataac atattatcaa aaaatataac
     5461 aaacttatat aggaggatta aaaatgagta aaaaaaataa atctctttta agtgaactta
     5521 agggacactt aatgacaggt atctcatata tgttgccact tgtaataggc tcatctttag
     5581 ttgtagctat cccaaagctt acagctttag caatgggtat aacaagttta gatgcttata
     5641 aaaatgtatc tggtttttat catattcttt acttaatgga acaagttggt tggactggca
     5701 taggtctatt aaatacggta ttagcaggat ttatagcatt ttcaataggt gagaaaccag
     5761 ctatgggtgc aggttttatt ggtgggctca tagcaagtaa tactaatgca ggattcttag
     5821 gagcagtaat agcaggtttc tttgctgggt atgtttgtaa gtttttaaag aaaaaaatta
     5881 aaatatctgg gtcagcatca ggaatgatgc cacttataat aatgccactt ataacagttg
     5941 gattaactgg ttttttaatg agtgttgtat tagcaggacc tttaggaaac ataaatactt
     6001 cattaaatgc ttgggtagct gaaatgtgtc aaaatggaac aaattcggtg gtattggctt
     6061 taattttagg cgcaatgata ggattcgatt taggtggacc agtaaataaa gcagcttgga
     6121 tggcaggaaa tgcattattg ttagagggaa tctatctacc agcgatattg gtaaactgcg
     6181 caatagttat acctccacta ggatatggtt tagctacatt cataagaaaa tcaaagtttt
     6241 ccaatgcatt agctgaaaca ggaaaaggca atatagttat gggtataatt ggaataacag
     6301 aaggtgcaat tcctttcact ttaacaaatc cattaaaatt agttcctgtt aatatgatag
     6361 gatgcgcttt gggtgtagga ttatcagctt tgttaggtgt acatgctata atgcctccag
     6421 ttggtggatt atatggattt atatcaatag gttctggatg ggcttactta gtaggtgcaa
     6481 tatttggagc tttagtaata gcaatattat caacaacttt agtagacttc aatgaagatg
     6541 aaactacgga aaatacagaa tttgatgaaa tagagttaga tataatgtaa attaaaaact
     6601 taattttaga taattgagaa ttgagtgtac tctcaattct caattaaatt ttgggaggat
     6661 ttttatgaaa aagacaaaag tacatgtagt tccacacagt cattgggata gagaatggta
     6721 ttttacaaca tcaaggtcaa aaatatattt aatgaaagat tttcaagata tattagacat
     6781 attggaagaa aaagaagact ttaaatactt tactatggat gcacaagctt ctctcttaga
     6841 tgattattta aaatggagac ctcaggatga atatagaata agagatttag ttcaaagaag
     6901 aagattagtt attggacctt ggtatactca aacagaccaa ttggttatat caggagaatc
     6961 tataattaga aacctttact atggaataga tagatgcaat gaatttggtg aacctatgag
     7021 ggttggatat gtaccagatt cttttgggca atcagctcaa atgccacaaa tatacagagg
     7081 ttttggaata gatagttcat tattttggcg tggagtttct gatgatatgg tagatacaac
     7141 agagtttata tggaaaggtt ctgatggttc aaaagtatta gcagttcaaa taccatttgg
     7201 ttattactat ggaggtaata taccagaaga tgattctgat ttaaaagaat atttaaaaga
     7261 tataatcgga aaacttaagg ttaaagcttc tactaataac gtatattttc caaatggatt
     7321 tgaccaagca ccaataagaa aaaaccttcc tgatatatta aaaaaagcta atgaaataga
     7381 taatgaaaat gaatacgaaa taagtgatat agaaaactat atagattcag taagaaatga
     7441 aagaaatgaa tttacagagc ttaaaggtga atttttaaat gctaaacata tgagaataca
     7501 caaatccata ttctcctcaa ggtcagacct taaaataatg aataacaaat tagaaaatta
     7561 tattacaaat gtacttgaac cattattaac attaagttat tcacttggaa atgaataccc
     7621 tcacctagtt ataaaagata tatggaaact tatgtttgaa aatgcagctc atgacagcat
     7681 aggttcttgt aatagtgaca caacaaatga ggatgtatac acaagataca aacaagccag
     7741 agatttgagt ataaatttat tagatttgca tatgagactt ataagtactc aaatcaaaaa
     7801 taataatgaa gaaataacat taacagtatt caatggattc ccagaagtca gaaatgaagt
     7861 tgtagaattt gatacttata ttccaggtca agattttgtg ataaagaata aaaatggaaa
     7921 aattcttaat tatataataa aagaaaaaga agatattaca aattatatat taactcaaac
     7981 aataagatta aacccaagta aaaaaatata tatacccaaa actgtatata gagcaaaaat
     8041 tgcactttca gtaaaagata taccaccaat gggatatact caactagttt ttgaattgga
     8101 tactaaaaat aaatgtgaaa tagaaacaag taattgcaag gaaatagaaa acaaatacta
     8161 caaaataaca gccaataaaa atggttcatt agaaatatta gataaagaat ctaataagac
     8221 ttataaaaac caagctgtaa ttgaagacaa tggagatgat ggagattcat acaactattc
     8281 tcctccaaga aaagatattt atgtttcatc tttagattct gtatcttcag tagaagtgct
     8341 aaaatcaaac attcaagaag aaatgatact taaatattca ttgaccatac caacttcact
     8401 ggaagaaagg gctataggta aagtttctgt gaaaatgcca gtaacaatga aaatcacctt
     8461 agaacctaaa gaacaaataa ttaagtttaa tgttaatata aaaaataacc aagcactatc
     8521 tcatagagta agagttttat ttaatacaga aatagcatct aagttttcta tagcagatca
     8581 acaatttgga ataatacaaa gaccaacagt attagaaaaa gaccttgcat tgtggaaaag
     8641 agacaattac tcatggcaag aaaaacctat aactatcgaa ccaatgcaaa gttttgttac
     8701 tttagaggat ggacaaagag gaatagcatt aataactggt tgtgtaagag aatatcaaat
     8761 agttggtgat aatttagata caatagcatt aacactattc aggtcatttg gatatatggg
     8821 aagagaaaat ttactttata gaccaggaag agcttctgga gaaaaaataa tagcaacacc
     8881 agatgcacaa ttacttaaag agttaaactt tgactttggt ctttacatat ataacaataa
     8941 atttgatgaa gcaaatgtag ctaatacagc taagagattc ttaacaccta tacaaatata
     9001 tgaatatgct gattttttaa atggtagact tatatttgca ttcaatgacg aaaaacaaat
     9061 atatgaaaat gaatttagtt tgatgaatgt aaataattca aatttcacag taagtgcaat
     9121 aaagaaagaa gaaaaaggag atggaattgt agttagaatc ttcaatggta tgaaaaatga
     9181 ggatgctaag ggaagtctta acatcaataa aaatgtcaat gatgcttata gtgcaatgct
     9241 cgacgaaatc tatgataata ctagcaagct taaagtaaat actaaaactg tagaggtaaa
     9301 ttctttatca cattgtaaag tacaaacaat agtaattaaa taatataaaa tagcttatac
     9361 aagatttcaa aataaattgc tagtactatg ttaatagtaa agccagatat tatgctattt
     9421 ataagctctg taacactaaa taacattttg taaaataaaa aggtgaacct tgtatattag
     9481 gattcacctt ttttaaataa atcaacattc tgttttaaaa taacatttat aagctgtttt
     9541 gaaataatgc ttacaaatta ttttaaaaca atacctataa atttttattt aaagaataca
     9601 cataaatatt tccagattct tttattctat agactttata attattaaag aagtctaatc
     9661 taatttttgt taaaagtaat tgtaactttg aataaatcac catccacact tatattcatc
     9721 ttaccaccct gtaattctat aaaacttttt gctatcgcta aaccaagacc agaaccttct
     9781 gtgttacgtg atttatctcc tcttacaaat ctctccatta tatcatctac actaaaatca
     9841 atctcagatg cagacatatt tttaagcata atatttactt tattatctgt ttctaaaata
     9901 tcaatataca ctcttgaatt tggcattgca tatttgctta tatttattat aagattttca
     9961 aatactctaa atgtacgctg actatctaat ggtaatatta ttttttcatt agaaaagtta
    10021 ttctttacta ttagagatga atctgcaagt ttgtcatcta attctaaaag tgtctgcttc
    10081 attagagata caatatccac atttactata ttcaaataaa catctccact atttgccttg
    10141 cttacttcaa acaaatcttc tattagatgt tgcaatcttt gtgactttct gtctaatgta
    10201 tcaatataca acttacgatt ttcatctgtt atactttcat cttttaataa atctatataa
    10261 gtgattatag atgttagagg tgtttttaag tcatgtgata catttgatat aagctctgtt
    10321 ttcatctttt gactcttaac ctcttcgtca acagcttttt taaatccagt ttgaatattt
    10381 cctaattcat cttttatagg attaaaaaat cctaaatctt tttccatatt tacatctaag
    10441 tttccatctg aaattttctt tgtaacatct aaaagttgat tatattcata tattgtcttt
    10501 ttagagtaat ctgacagtat cttaaataat actactgaat atattaatgc gagtattatt
    10561 ccaaagaacc acatactaca cattataact ataatcaata aatttattcc caaaattgta
    10621 actatagttt tatttggctt ttctttcaga tttattgttc taatttgatt aataactgct
    10681 tttcctgctt tttttaacca tacaaaaaac attcctgtca cagtatgttt tgtaaaatac
    10741 tctcttattc caacatttaa aatgtgtttt aaaagtatta taaatacaaa tattattgag
    10801 aatattacaa accaataagt taaattcatt aaacttacca atatactagc cattttagca
    10861 cttatgtcat tgtttataag gtactctaca taattcccac tcaaggtatt acttattata
    10921 acagtaggat ataaatctac aagcccctgt ataatagcaa gagcaataaa gagtatctct
    10981 aacggtattc ttgataatgt atcaaatcct agcaactctt tagattttct atatgggaat
    11041 gctaaagcta caataacaac tatagctaat ataattttta aaatcattcc agaaatctta
    11101 taataagaat tattttctac attgcttata tatgaaatta taccatcatc aaaataagta
    11161 tttccataac catactctac actatcacct ttacttatta ctttaggtac agcatatact
    11221 aatgttatat tttttattgg agataactta tatgaaattg aatttcctcc taaacttgta
    11281 tccattaaat cttctcttgc tttagataac aacatttgat taataatttt ttgatttact
    11341 ccataagaat ccacaatatt tacttttcca tctttatcaa aattcattac aacgtagaat
    11401 ctatagtttt cttttatatc tgtaatctgc ttttgtcttt cctcatcatt catttctgta
    11461 ttttttgact gattttcatt agaattttct tttgaagcat cattttttgt tgtatcaagt
    11521 actcctaatt ctttattatc attaaaaaat tcaaaattgc tttctttatc aactaaatag
    11581 tactttaaat tctttcctga cgctttaatg aaatgctccc aattttcaaa gttttcatta
    11641 aaatctgcat taatactatc atctgtataa taagtttctg aaccatcata agtatctttt
    11701 tctttactta cattttttat atagttcata gtttcttcat tgataaatag ctttgatggt
    11761 tgtatcatct taccttcttg ttcttccttc atattatgat ataaaacata acttccttta
    11821 tatatactat ctacaaagct ttcctgttca aatggattct ctgctccacc tttagcactt
    11881 gcttgtatag tagaatattg actaatcata cctaatgatg ctgataacat aagtataaaa
    11941 actataatag taactatata tttatcttta aattttttca attttgtatc caacacccca
    12001 caccaccttt aaatatttag gattttttgg gtcaatttct atcttttccc ttatattcct
    12061 cacatgtacc ataactgtat ctgtatttac agcattctca ttccaaactc tttcatatat
    12121 ttcatcagca gaaaaaactc ttcctgcatt tctcataagt aaacttagta ttttaaattc
    12181 tataggagtt gtttttatat actttccatc aatactaact tcttttgtat tttcatttaa
    12241 ttctaatccg cctattgcaa acacactatc gtcactttct accttttgct ctttatttcc
    12301 aaccatgttt agatacttag tgtaccttct aagttgtgaa ttaactctag ccaatagttc
    12361 taatggctta aaaggttttg ttacataatc gtctgctcct atattaagcc ccattatttt
    12421 gtcaacttct tctgattttg cagatagcat tattactgga aaattatagt tttctctaag
    12481 cttcattgta agtgttatac catccatttt aggcatcatt atatcaacta tagctaaatg
    12541 tatttcttgg ttttctataa ccttaagacc ttctaatcca ttgtatccct taaaaacatt
    12601 atatccttga tttaatagat atatttctat agcatctgct atctcctttt catcttctac
    12661 caccaaaata ttatatgaat tcattctaat ctcctttata ttaatttata aattttcttt
    12721 acatatatga ttatacaata tgatagaagt ttttccattt atatatatat ttatatcttt
    12781 tgttctggtt atttgtattt aatgtccatc ttagaaacct ccttctagtt t
//
LOCUS       DAMJWU010000102        12623 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMJWU010000102 DAMJWU010000000
VERSION     DAMJWU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.106"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(<1..132)
                     /locus_tag="PC460_003015"
     CDS             complement(<1..132)
                     /locus_tag="PC460_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601884.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYEAGSREPN
                     "
     gene            245..439
                     /locus_tag="PC460_003016"
     CDS             245..439
                     /locus_tag="PC460_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601885.1"
                     /translation="MKINNNLKLQREKIGLTQLEVAQKAKITERSYQYYEAGERLPNI
                     RTALKIAIILNTNCEKLFNE"
     gene            481..1275
                     /locus_tag="PC460_003017"
     CDS             481..1275
                     /locus_tag="PC460_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDF2601886.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEIRHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSTYLDSKNESRPCYNVTKLGCDFLANKFTGEKGIIFTARYVRRF
                     NEMERVLKEQQPKLPTTYKEALQQLLVEVEEKEQLQLENQEKDKVIQLQQPKVLFADA
                     VSASNNSILVGELAKLIKQNGVDIGQNRLFTWMRENGYLIKRKGEDYNIPTQKSMNLR
                     IMEVKKRVINNPDGSTKVTRTVKITGKGQVYFVNKFKSSKQLSMLS"
     gene            1332..1541
                     /locus_tag="PC460_003018"
     CDS             1332..1541
                     /locus_tag="PC460_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601887.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(1551..1925)
                     /locus_tag="PC460_003019"
     CDS             complement(1551..1925)
                     /locus_tag="PC460_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601888.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            2001..2198
                     /locus_tag="PC460_003020"
     CDS             2001..2198
                     /locus_tag="PC460_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601889.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELNIDVSGLYKLLKNKNSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            2229..2621
                     /locus_tag="PC460_003021"
     CDS             2229..2621
                     /locus_tag="PC460_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601890.1"
                     /translation="MDISESITKQFSDSLKSLIEIEINKQETDRVKSQTVEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTVFMRRLVSTGLIKSLARGDGRKISRYEV
                     DDFIERNQGKNLDELLKVAERGDEIVKP"
     gene            2608..2751
                     /locus_tag="PC460_003022"
     CDS             2608..2751
                     /locus_tag="PC460_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601891.1"
                     /translation="MLSLNTNKNNIVTLKKDGKAIADIVFKDIKTGKKISVGVLNKKV
                     LVK"
     gene            2918..3160
                     /locus_tag="PC460_003023"
     CDS             2918..3160
                     /locus_tag="PC460_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601892.1"
                     /translation="MEAARLIAIGQIKQAEKEICKLQGTKNNSSLMWWEAVKFASQNI
                     LEGLEHDIELEASIEFREAMMYQEELEKDRPIDVQI"
     gene            3239..3520
                     /locus_tag="PC460_003024"
     CDS             3239..3520
                     /locus_tag="PC460_003024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601893.1"
                     /translation="MKTKNEIIKGLEDRLFLLRFTTVDEVDWDVKFGQISALEFCIDK
                     HRKGCTLEQFKEHLDEYKLQGNYGDYIDGFVSVLERNIREMEGEIDGSE"
     gene            3507..4145
                     /locus_tag="PC460_003025"
     CDS             3507..4145
                     /locus_tag="PC460_003025"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF2601894.1"
                     /translation="MEVNNIYIKLMDVRIKFNKLDIKKSGQNKFANFKYFELADFLPQ
                     ATELLQEAKLCPIVTFTNDYATLTLINGEKPTEEIIFTSPMRELQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDTFDATSGKEDYKRNDLTNSSIKASENGQIKISQNQIKRL
                     FSIGNTIGKDSDRVKSEVYYKFNKEVKDLSKQEYDQICVGYEKLQREKGIIK"
     gene            4155..5050
                     /locus_tag="PC460_003026"
                     /pseudo
     CDS             4155..5050
                     /locus_tag="PC460_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
     gene            5070..5489
                     /gene="ssb"
                     /locus_tag="PC460_003027"
     CDS             5070..5489
                     /gene="ssb"
                     /locus_tag="PC460_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2601895.1"
                     /translation="MNQVVLVGRLTRDPELKYIPGTGTAVASFTIAVDRNYINKEGKR
                     DTDFIPIEVIGKSAEYCANYITKGKLVALEGNIRVDNYQTQSGEKRIFTKVSTKSVQS
                     LESKNKSSNSYKESVQDGTIGLDPQGFEIIDDDELPF"
     gene            5519..5881
                     /locus_tag="PC460_003028"
     CDS             5519..5881
                     /locus_tag="PC460_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601896.1"
                     /translation="MFKVERYFSGSVVDNLIEDDLTCRNYLALYCCLLGITKNGKKIY
                     PKPEKMLAEFGVKKDRKIKKELPVRIRNVNTGEVKEFESIDGAACFLRLKYQAVYQAI
                     KKKTKTRSGWKVEYIKEE"
     gene            6115..6966
                     /locus_tag="PC460_003029"
     CDS             6115..6966
                     /locus_tag="PC460_003029"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2601897.1"
                     /translation="MNVLSLFDGISCGQVAFERARIKVENYFASEIKEIAIKVAMNNY
                     PKTIQLGDVRELNVSKIPKVDILIGGSPCQNLSKGRLIANKIQDGLTGDKSNLFWEYI
                     RILKQLNPEYFLLENVVMPKKDENMISQLLGVEPIKINSNLVSYQNRDRLYWTNILNV
                     QQPKDLKISFQDYKDTSFDYCKEFEIKKTPSREKMWGNGLNGNCKNVTYEDKINCVTL
                     KQDRFSNSGLVEFGSFCRYLTTRELELAQTLPVGYTKGLSIRQAQNVIGDGWTIDVIA
                     HILSNIN"
     gene            7283..7480
                     /locus_tag="PC460_003030"
     CDS             7283..7480
                     /locus_tag="PC460_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601898.1"
                     /translation="MNKFQKAVSQMVKQEEKENLLQGYENCIVGRSISSSIRRYVKGF
                     EKFGYSVHEVYEFINDINKYE"
     gene            7507..7923
                     /locus_tag="PC460_003031"
     CDS             7507..7923
                     /locus_tag="PC460_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDF2601899.1"
                     /translation="MSKYNNKKIVIDGIKFDSKDESEYYLYLKEKKENGEIKDFGLQQ
                     KFELQPKFKKDGKSYRAITYTIDFAIYKWNGEVVYIDVKGYSTQQGELRKKLFDYKYQ
                     DKKLIWIAKSKKYGVDGWIEYSELKKKRKENKKKVA"
     gene            7942..8442
                     /locus_tag="PC460_003032"
     CDS             7942..8442
                     /locus_tag="PC460_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601900.1"
                     /translation="MASKVKKEFFMATKKHLENYKQLHINIESLKLQIKNLKEFHLGD
                     FMQGLSYDSIPISKTNSISNQVENELINLEEKIIEKQIELYEMEALKYTIDVSISNLK
                     PIHKQIIRYRYIEGLEWSLIVDKVYLEERQLRERANQAISSISIALFGKKALIEQEPL
                     FKMLDL"
     gene            8864..9070
                     /locus_tag="PC460_003033"
     CDS             8864..9070
                     /locus_tag="PC460_003033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601901.1"
                     /translation="MNRFEGIYSFAEATKLWDLKDSTLRKAVATGKLVENIDCKKFGR
                     DWVVTIEAMEREYGKLNNIMKDLD"
     gene            9559..10746
                     /locus_tag="PC460_003034"
     CDS             9559..10746
                     /locus_tag="PC460_003034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601902.1"
                     /translation="MLNEKNLKNIKNILQMVQMKHLKDFENWGINKRKKETAINSLIN
                     IIQNDGEKLDYFKEWISIFTIDGHNNYYVFNYNDSDLELEDLKNIVEDIVSENLLEID
                     TSDLEETKVIYKKNDVINKRLIIRFISPALISCKNENENGNIESGLEKTFYFATLFLD
                     LSLKQIIVSIPHTVGIKSIDNIEAKARDFSEIAKYYVSKLEDLLTTYNIQIEKYNDWI
                     YDATYELAEEGSAHNNPEINKKYEENIDKIDEFAKKIAIASGITDKAIIENFKEGVGI
                     LYENILIDEFGVVEDENQYSTFVQNGDGVNSYCRVGSKTATLKSGKGHAIAKTSRNNE
                     DVKNLGVMKSFNGTLSRFLIEVLDKELYLIRTDTSKFIEERVIYDVIRKVGEYKYTFR
                     KSE"
     gene            10691..11077
                     /locus_tag="PC460_003035"
     CDS             10691..11077
                     /locus_tag="PC460_003035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601903.1"
                     /translation="MMLYEKLENISILLGNQSKAKSIIEELEKLMKSDIITPRLLARK
                     TKVTLLEATRVLEFLVKENELEFFIVVECSNPDSVYENEIGHYKYFTSIKEFNAFSKG
                     QECPICGCGYKYNFEDTKIGFRRLEK"
     gene            11082..11852
                     /locus_tag="PC460_003036"
     CDS             11082..11852
                     /locus_tag="PC460_003036"
                     /inference="COORDINATES: protein motif:HMM:NF016362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDF2601904.1"
                     /translation="MSLDTLKEYLSICDDNLIWEPFYDDSMLLDEFSNYIKEIELSKE
                     WNKSKNHEKGKILEQLVRFIMSRFTILSDISVNKSTTDNELDVFVKFNDNIPIPFLCN
                     IKSKIVCECKNWKSRKVDVGMVSKLAEICNKNNAGLGIFISLNGLTGKGWQYAEGKRR
                     KLYLSDKMPIISFTVDEITKLKYKRYNLLTMIKCKMQALIDEMEFAGGELRLLESELG
                     FIDCLNENIESLKMLGLITEVEYNNIKVRINHRYDIVN"
     gene            11910..12593
                     /locus_tag="PC460_003037"
     CDS             11910..12593
                     /locus_tag="PC460_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601905.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWANE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKDKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKYESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDRNDS"
BASE COUNT         5388 a         1268 c         2320 g         3647 t
ORIGIN      
        1 atttggttct cttgaaccag cttcataata ctgatatgca cgttctgaga taccaaacaa
       61 actagcaaat tgcttttgag tcatattcat ttgctttctt actagcttaa tattatcttt
      121 gaatttagtc ataactacac gctccaataa aaaaataaat aaatttcttg acacgaacaa
      181 tattagcgtg taatatataa aacatagcac gaacaatatt ggcgtgttaa ataaaaagga
      241 gagggtgaaa ataaataata atttaaaact acaacgtgaa aaaattggct taacacaatt
      301 agaggttgct caaaaagcca aaataacaga gagaagctat caatattatg aagctggcga
      361 acgtctccca aacattcgta cagctttaaa gatagctata attttaaata ctaattgtga
      421 aaaactcttt aatgaataat agcataaaag gactcagatt acaagaaagg agctttagat
      481 atgaataatt tgcaacttaa caataaaaat acaataacaa cattagaaat tgctgatatg
      541 ttagaaatca gacactggga agtcttaagg aaattagaag gaacagaaaa aacaaaagga
      601 attattgata ttcttaacga caacaatttt gttgtggttg attactttat aaaatcaaca
      661 tatttggatt caaaaaatga aagtagacct tgctataatg taacaaaatt aggttgtgac
      721 tttctagcaa ataaatttac tggagaaaaa ggtattattt ttacagctag atatgtaaga
      781 agatttaatg aaatggaacg agtattaaaa gaacaacaac ctaaactacc aactacatat
      841 aaagaagcat tgcaacagtt attagtagaa gttgaagaaa aagaacaatt acaattagaa
      901 aatcaagaaa aagataaggt aattcagtta cagcaaccaa aagtactgtt tgctgatgca
      961 gtttcagctt caaacaattc tatattagtt ggagaacttg caaaacttat aaaacaaaat
     1021 ggagtagata taggacaaaa cagattattt acctggatgc gagaaaatgg atatttaatt
     1081 aagcgtaaag gtgaagatta taatattcca acacagaaaa gtatgaatct aagaataatg
     1141 gaagttaaaa aaagagtaat taataatcct gatggaagta ctaaagtaac tagaacagta
     1201 aaaataactg gaaaaggtca agtttatttt gttaataagt ttaaaagtag taaacaatta
     1261 tcaatgttaa gttaaatcat aacagtacct tgaaaactaa atacagaata ttttggaaag
     1321 gagagattga attggaaaat aagaaaatag aagaaataga gaaaagaata accatgcttg
     1381 aaaatcaatt gcaagcacag ttatttagtc agataacagc tcaatcaaat agtgatttta
     1441 tagattatat gtaccaatgt atagttttgt atttacatga gatgaataaa agtgaaaaaa
     1501 gaaaaattag tgaaattact aaagaaagaa tcagaaaata agaatgataa ttacaatata
     1561 aaacctattc ttctagcagg ttttgaaggg tcagttttct ttactgaagt tatcataaag
     1621 tttatctgtg atgtgtgttg aagaatttca gaataaactc catttactat gccgtagtag
     1681 tgaattagac aagggttatg ataacctact tcttcaacaa tcattaatac atcttttcca
     1741 aagttagtca attttagtgc aatttcatgt tcatcatcaa gttctttttc aaattcctct
     1801 atagatttaa gaataatttt atattgaaat tcagcatagt aaaaatactc tgtgttagaa
     1861 gtatcagaga ctgtctttct tatactttct tgtaattctt tagatatatt gtttgaaaac
     1921 tgcataatac accatccttt tgaaaatttt ggaatttatt ccatgtttat attataccat
     1981 gtagaactga ggtgaataca atgttaatag gcgacaatat aaagcaaata ctaagaaaaa
     2041 gagatataaa accttataaa ttagcaaagg aattaaatat agatgtaagt ggtctatata
     2101 aattgttgaa gaataaaaat tctaatccaa ctatagatac tctaataaaa ttagctgatt
     2161 atttagatat tacattagac gaattagttg gaagataaat atttgaaaag aggggagaga
     2221 attttaacat ggatatttct gaaagtataa caaagcaatt tagtgatagt ttaaaaagtt
     2281 taatagaaat agagataaat aaacaagaaa cagatagagt taagagtcaa actgttgaac
     2341 aaaaggtaaa agtactggag cctaaagata tagttgtttt aataaaaaga ggttatccaa
     2401 attatttgat aacagtagaa gaagcaaggg gaattttaaa attagataca gtttttatgc
     2461 gtaggttagt gagcacaggt ttgataaaat cactggctag aggtgatggt agaaaaattt
     2521 caagatatga agttgatgat tttattgaaa gaaatcaagg taaaaatttg gatgaacttt
     2581 taaaagtagc agagaggggg gatgaaattg ttaagcctta atactaataa gaataatata
     2641 gtaactctta aaaaagatgg aaaagctata gcagacatag tatttaaaga tattaaaact
     2701 ggtaagaaaa tatcggttgg agtattaaat aaaaaagtgc tggtcaaata accaacacac
     2761 aaaaaaataa aaaataaaat ataacacaaa caaattatag cacaaaaaga atggatttag
     2821 aataggtaat ataacattct aaaaggttta atgtgtaata attgtaacaa tttagaatgt
     2881 agcttatata gatagtaata aattgggagg gatttaaatg gaagcagcta gactaatagc
     2941 aataggtcaa attaaacaag cagaaaaaga aatatgtaaa ttacaaggta caaaaaataa
     3001 tagtagttta atgtggtggg aagccgtaaa atttgctagt caaaatatat tagagggtct
     3061 tgaacatgac attgagttag aagcttctat tgagtttaga gaagctatga tgtatcaaga
     3121 agaacttgaa aaagatagac caatagatgt ccagatataa aaaaagagcc ttcgcacagg
     3181 ctctaagtga aaataagtta caaaaattat aggtatatta taacataagg gggaataaat
     3241 gaaaacaaaa aatgaaataa ttaagggttt agaagataga ttatttttat taagatttac
     3301 aacagtagat gaagtagatt gggatgtaaa atttggacaa atatcagcat tagaattttg
     3361 tatagataaa catagaaaag gatgcacttt ggaacaattc aaagaacatt tagacgaata
     3421 caaattacaa gggaactatg gtgattatat agatggtttt gtgtcagttt tagaaagaaa
     3481 tattagagaa atggagggag aaattgatgg aagtgaataa tatttacata aaattgatgg
     3541 atgtaagaat taagtttaac aaattagata taaaaaagag tggtcaaaac aagtttgcta
     3601 actttaaata ttttgagtta gcagacttct tacctcaagc aacagagctg ttacaagaag
     3661 ctaaattatg ccctatagtg acctttacaa atgattatgc aactctaaca ttgattaatg
     3721 gagagaaacc aacagaagag ataatattta cttctccaat gagagaatta caattaaaag
     3781 gctctaatga gttacaagca ttaggaggaa ttgaaacata tcagacaagg tatttgtaca
     3841 ttcagttatt aaacattaca gaaaatgata cttttgatgc tactagtgga aaagaagatt
     3901 ataaaagaaa tgacttaaca aactcctcta ttaaagcaag tgaaaatggt caaataaaaa
     3961 taagtcaaaa tcaaataaaa agattgtttt caataggaaa tacaataggc aaagattcag
     4021 atagagtaaa aagtgaagtg tattataagt ttaataaaga agttaaagat ttaagtaaac
     4081 aggaatatga ccagatatgt gttggatatg agaaattaca aagggaaaaa ggaataatta
     4141 agtaggtgaa tttcttgaag gacagagaca aagcaactat agagaaagga aatatactta
     4201 gtgatgggta tggtctttca ccacaattgg tagctagaga ttcatggcta acaacaggag
     4261 caagagcttt atatttctat ctatccagtt ttgcaggagc aagtgggaca tgttatccat
     4321 ctagggatat tatgactcat gaacttggta taaataaaga tacttttagt aaatacctaa
     4381 atgaactaaa gacgagtggt tatataaaag tatataaaaa taaaactagg gaaggaagga
     4441 tgcaaaataa tatatatgag gtagtatttg atagaagcta tatagaaagt catatatcca
     4501 ttagatgtaa gaaagaaaat aaaaaaaacc atgtccgaaa aaagcagaca tggaaccatg
     4561 tccgaattta ccggacatgg ttcaaccgga catggaaaag tcggacacta taagtaacag
     4621 tattataaat aacagtttta aaagtagtat gtatatagag caagctgtgg ataactcttt
     4681 aaaagaattt aagaagctat atgaagaaaa tataggagta gtatatccag tcacagctga
     4741 atggttatta gaagtatcta atgaagtaga tataagagta tttaaaagag ctatagagat
     4801 atgtgctgaa agaatgaata tgaatttatc atacttaaaa ggaatcctta aaaagtggaa
     4861 ggatgcaaat ataactacat atgaacagtt agaatcatat aaattacaac atgaaaataa
     4921 aaagtcaaaa aaaaccaata gtgtagtaag caaaaataag tttgctaatt ttgaacaaac
     4981 atttactcaa tacagtaaca aagaattaga tgaaattata aagaaaagcc aaaaggctaa
     5041 atttaaataa tattgatgga ggtattaaaa tgaatcaagt tgtattagtt ggaagattaa
     5101 ctagagaccc agaactcaaa tacataccag gaacaggtac agcagtagca tcatttacaa
     5161 tagctgtaga cagaaattat ataaataaag aaggaaaaag ggatactgat tttataccaa
     5221 tagaagtaat aggtaaatca gctgaatact gtgcaaatta cataacaaaa gggaagctag
     5281 tagcattaga agggaatata agagttgaca attatcaaac tcaatcaggt gaaaaaagaa
     5341 tatttacaaa agtcagtaca aaatcagtac aatcattaga aagcaagaat aaatcgagta
     5401 attcatataa agagagtgta caagatggaa ccataggact agaccctcaa ggatttgaaa
     5461 ttatagatga tgatgagtta ccattttaat ctgaaaaatt aaatatgagg tgaaataaat
     5521 gtttaaagta gaaaggtatt ttagtggctc agtagtggac aaccttattg aagatgacct
     5581 tacatgtaga aactacttag cattatattg ttgtttgttg ggaattacaa aaaatggaaa
     5641 aaagatatat cctaagccag aaaaaatgtt agctgagttt ggagtaaaga aggacagaaa
     5701 aataaaaaaa gagttaccag taagaattag aaatgttaat acaggggaag taaaagaatt
     5761 tgagtctata gatggtgcag cctgtttttt aagattaaaa tatcaagcag tttatcaagc
     5821 tattaaaaag aaaactaaaa caagaagtgg ctggaaagtt gaatacatta aggaggaata
     5881 atggaagttt caaggacaga atatacaatt aaaagagcaa aagagttata tgacaatgga
     5941 gaggatatat ttattgctat agataaggct agagaaaaat atgaggagat gattaaaagt
     6001 gaatatctta gctagtgtga tattagtaat aggaagtttt atagctggta gagtttatga
     6061 gtatagattg aacctaaaag ggtgtgaaaa ttgtgataat aagaaggtgc aaatatgaat
     6121 gtattaagtc tattcgatgg aattagttgt ggtcaagttg cttttgagag agctagaata
     6181 aaagtagaaa attattttgc aagtgaaata aaagagattg cgattaaagt agcaatgaat
     6241 aattacccaa aaacaataca acttggagat gtaagagagc taaatgtaag taaaatccct
     6301 aaagtagata ttcttattgg cggaagccca tgccaaaatt taagtaaagg aagattaata
     6361 gctaataaaa tacaggatgg attaactgga gataaaagta atttgttttg ggaatatata
     6421 agaattctaa aacaattgaa tccagaatat tttcttttag aaaacgtagt gatgcccaaa
     6481 aaagatgaaa acatgataag ccaattgtta ggagttgaac caatcaagat aaatagcaat
     6541 ttagttagtt atcaaaatag agacagatta tattggacaa atattttaaa tgttcaacaa
     6601 cctaaagatt taaaaattag ttttcaagat tataaagata ctagttttga ttattgcaaa
     6661 gagtttgaaa ttaaaaaaac tccaagtaga gaaaaaatgt ggggtaatgg tttaaatgga
     6721 aattgtaaga atgtaacata tgaagacaaa ataaactgcg taacattaaa acaagataga
     6781 tttagtaatt caggtttagt agaatttggg tctttctgca gatacttaac aactagagaa
     6841 ttagagttag cgcagacgct accagttgga tatacaaaag gtttgagtat aagacaagca
     6901 caaaatgtca taggtgatgg ttggacaata gatgtgatag cacatatttt atcaaatatt
     6961 aactagttct ttatatataa attattattg aagcggttag ctatcatgat acttgtagca
     7021 gtaagtttta tagctggtag ggtttatgag tatagattga atctgaaaga gtgtgaaaat
     7081 tgtaataaca actatcctga aaaatgagaa agaagtggtt ttatgaataa aagaataatt
     7141 tgcaattggt gtggtaaatt attttacatc ccaaaacagt ctaaaaaaat ttactgttgt
     7201 aaaagatgtg agagaaaggc taagaaaagc aatagagaac agcaaaatta attttaaaca
     7261 ataaaactat gggggaatag caatgaataa gtttcaaaaa gcagtttctc aaatggtgaa
     7321 gcaagaggaa aaagaaaact tgttgcaagg atatgagaat tgtatagtag gtagaagtat
     7381 atcaagttcc ataagaagat atgtaaaagg gtttgaaaag tttggatata gtgtacatga
     7441 ggtttatgaa tttataaacg atattaataa gtatgagtaa ctttaagtga taaaggaagt
     7501 atttgattga gtaaatacaa taataagaaa attgtaatag atggaattaa atttgatagt
     7561 aaagatgagt cagagtatta tttatattta aaagaaaaaa aggaaaatgg agaaataaaa
     7621 gactttggac ttcaacaaaa gtttgaacta caacctaaat ttaaaaaaga tgggaaaagc
     7681 tatagagcta ttacatatac aattgatttt gccatataca aatggaatgg tgaagtcgtt
     7741 tatatagatg tgaaagggta tagtacacag cagggtgaac ttagaaaaaa gctttttgac
     7801 tataaatatc aggacaaaaa attgatatgg attgctaaaa gcaaaaaata tggggtagat
     7861 ggttggatag aatatagtga acttaagaaa aagagaaaag aaaataagaa aaaggtagct
     7921 taaataaata ggagtgatgt tatggcaagt aaagttaaaa aggagttttt tatggcaact
     7981 aaaaaacacc ttgagaacta caaacaacta catattaata ttgaaagtct aaaacttcaa
     8041 ataaaaaatc tcaaagagtt ccatttaggt gattttatgc aaggtttaag ctatgacagc
     8101 attcccataa gtaagactaa ttcaataagt aatcaagttg aaaatgagtt aattaatctt
     8161 gaagaaaaga taatagaaaa gcagatagaa ttatatgaaa tggaagcact aaaatataca
     8221 atagatgtat ccataagtaa tttaaaacct atacataaac aaattataag gtataggtat
     8281 attgaaggct tagaatggag tttaatagtt gataaagtat acttagaaga aagacaatta
     8341 agagaaagag ctaatcaagc cattagttca atatcaatag ccttatttgg taaaaaagca
     8401 ttaatagagc aagaaccatt atttaagatg ttagatttat aggcagttaa taactgtcta
     8461 ttttttttgt gaaaaagata tatggaagtt attgacaata tcacgcatac gtgatattat
     8521 tgaagtatag aaaggaggcg aaaagaaagt ggctaagaaa ataaaagagt tcagaaaact
     8581 cataaaagaa ttaactgaac tcgtactcga agttggcaca ctattagcag tcatcaaaat
     8641 ggtaatagat agcctacatt agtaaaatgt taagagtgga agttgcacct tccctcttaa
     8701 ctaaattata aaacatagtc actaaaaata caatgaataa gtatagagag ttaacatttg
     8761 aattattgaa actactgtat gacactatta aattaattgg agcatcaata ttgttatatt
     8821 atgttattaa ctcaattttc tagcaatgat taggaggtat tttatgaatc gatttgaagg
     8881 tatttatagc tttgcagaag caacaaaatt atgggatttg aaagattcta cacttagaaa
     8941 agcagttgcg acaggcaaat tagttgaaaa tattgattgt aaaaagtttg gtcgtgattg
     9001 ggtagtaact attgaagcaa tggaaagaga atatggaaaa ttaaataata taatgaaaga
     9061 cctagattaa attctaggtc tttttattgc cgcttttctg ccgattttac aatttaaaat
     9121 gtgagataat agtattgtgg aaatgaatat ttctctctca aaactaaata tatgtgggct
     9181 aggttaaggg attcgcctag cttatatgaa caaactaggc agggcatgag gatgctgtaa
     9241 gttcaattct tactatgttc aaacttatta atacactata tgtagatatg ctggattaaa
     9301 acggaattta attcaaatgt ctaaaagagt ggggcttggt aacctcactc aatttgcaag
     9361 gactggtgtg taatcttagg ttcgattcct aaaacttgct cccttaaata taatatgtat
     9421 ccccaaaaaa aggcttagat taaattctaa gtcttttttt aataattaat gtagataata
     9481 atatttattg ctaaaatgat gtacaaatat gataatataa attacataaa gatatataaa
     9541 tagaggggga ttataaattt gttaaatgaa aagaatctaa agaacataaa gaatatatta
     9601 caaatggtac aaatgaaaca ccttaaagat tttgaaaatt ggggaattaa taaacgaaaa
     9661 aaagaaactg ctataaatag cttgataaac ataatacaaa atgatggaga aaaactagat
     9721 tattttaagg aatggatatc aatctttaca atagatggtc ataataatta ttatgttttt
     9781 aattacaatg attctgactt ggaattggaa gatttaaaaa acatagtaga agatatagta
     9841 agtgaaaatt tattggagat tgatactagt gatttagaag aaactaaggt catatataaa
     9901 aaaaatgatg tcataaacaa aagattgatt ataagattta tttctccagc tttaattagc
     9961 tgtaaaaatg aaaatgagaa tgggaatata gaatctggat tagaaaaaac tttttatttt
    10021 gcaactttat ttctagactt aagtttgaaa caaataatag tttctatacc acatactgta
    10081 ggtataaaaa gcatagataa tatagaggct aaagcaagag atttctcaga aatagcaaaa
    10141 tattatgtat caaaattaga agatttgtta actacttata atatacaaat agaaaaatac
    10201 aatgattgga tatatgatgc tacatatgag cttgctgagg aaggcagtgc tcataataat
    10261 cctgagataa acaaaaaata tgaagaaaat attgataaaa tagatgaatt cgcaaaaaag
    10321 atagctattg cttctggaat aactgataag gctataatag aaaatttcaa agagggagtg
    10381 ggaatactct atgagaatat tctaatagac gaatttggtg tggtagagga tgaaaatcaa
    10441 tacagtactt ttgtgcaaaa tggtgatgga gtaaattcat actgtagagt tgggtctaaa
    10501 acagcaaccc taaaatcagg aaagggacat gctatagcca agacatctag aaacaatgag
    10561 gatgtcaaga atttaggtgt catgaaatca ttcaatggaa ctttatccag atttttaatt
    10621 gaagtcttag ataaagaatt atatttaata agaacagaca cttctaaatt tatagaagag
    10681 agggtgattt atgatgttat acgaaaagtt ggagaatata agtatacttt taggaaatca
    10741 gagtaaagca aaatctataa tagaagaatt agagaaatta atgaagagtg atatcattac
    10801 accaagattg ctggccagga aaactaaagt tacactttta gaggctacaa gagttcttga
    10861 atttttagtt aaagaaaatg aattagaatt ttttattgtt gttgaatgtt ctaatccaga
    10921 tagcgtatat gaaaatgaaa taggtcacta taaatacttc acttcgataa aagaattcaa
    10981 tgctttttca aaaggacaag aatgcccaat atgtggatgt ggttataaat acaactttga
    11041 agatacaaaa ataggcttta gaagattgga gaagtgatac agtgagtcta gataccttaa
    11101 aagaatatct aagcatatgt gatgataatt taatttggga gcctttttat gatgacagta
    11161 tgctattaga cgaattctct aattatataa aagaaataga attatctaag gaatggaata
    11221 aatctaaaaa tcatgagaaa ggaaaaattt tagaacaatt agtaagattt ataatgtcta
    11281 gatttacaat attaagtgat atatctgtaa ataagtctac aacagataat gagctagatg
    11341 tttttgttaa gtttaatgac aacataccaa taccattctt atgtaatata aaatcaaaaa
    11401 tagtatgtga atgtaaaaac tggaaaagta gaaaagtgga tgtaggaatg gtatctaaac
    11461 tagcagaaat atgcaataaa aataatgcgg gattgggcat atttatttct ttaaatggat
    11521 tgacaggtaa ggggtggcaa tatgctgaag gaaaaagaag aaaattatat ttatcagata
    11581 agatgccaat aatatcgttt acagtagatg aaataacgaa attaaagtat aaaaggtata
    11641 atcttctgac tatgataaaa tgtaaaatgc aagcattaat agatgagatg gaatttgctg
    11701 gaggagaatt aagattatta gaaagtgaac ttggatttat agactgttta aatgaaaata
    11761 tagaatcttt gaaaatgttg ggtttaataa ccgaagttga atataacaat attaaagtaa
    11821 gaatcaatca tagatatgat attgtaaact gagaactcca aacgagttct ttttttattc
    11881 ccaaaacgac aaacaaacga ggtggtggtg tgcaagatgt gaaagaaaaa gtaaaacaag
    11941 attacctaaa aggtatgaaa caaaaggaaa tatcagcaaa gtatgacatt agtttaaata
    12001 ctttaaagtc atggataaaa agatacaatt gggctaatga gaaaaagaag ggtgcaccta
    12061 aaaataaaag aggtgcaccc ataggtaata aaaatgccac tggtcctcct ggaaataaga
    12121 atgctgaaaa atttggtttc ttctcaaaat acttacctga agaaacccaa gacttaatta
    12181 atgagataaa ggataaagat aaatttgata ttctttggga acagataaca atacaatatg
    12241 cagcaataat aagagcacaa aagataatgt atgttaaaga caaggaagaa atgattaagg
    12301 aattaaagaa atatgaaagc acagaaaatg gtgagaagat agagtatgaa tttcaatttg
    12361 catgggatag gcaagcatct tttcttaatg cacagagtag agcaatgagt gaacttagaa
    12421 gtttaattaa acagtatgat gaaatgattc ataaggattg gaatttggct acagaggagc
    12481 agaaaacaag agttgagaag ttgaaatgtg aagttgataa cctaagtaaa gatgatattg
    12541 gagatgatga gttgaaaata agtgtagatt atggtgatag aaatgatagt tagaatagat
    12601 tttaatccag atttcaagga agc
//
LOCUS       DAMJWU010000103        12531 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMJWU010000103 DAMJWU010000000
VERSION     DAMJWU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.107"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(142..1356)
                     /gene="dpaL"
                     /locus_tag="PC460_003038"
     CDS             complement(142..1356)
                     /gene="dpaL"
                     /locus_tag="PC460_003038"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2601906.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(1970..2507)
                     /locus_tag="PC460_003039"
                     /pseudo
     CDS             complement(1970..2507)
                     /locus_tag="PC460_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(2584..4389)
                     /locus_tag="PC460_003040"
     CDS             complement(2584..4389)
                     /locus_tag="PC460_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2601907.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(4785..5162)
                     /locus_tag="PC460_003041"
     CDS             complement(4785..5162)
                     /locus_tag="PC460_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2601908.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6067..6552
                     /locus_tag="PC460_003042"
     CDS             6067..6552
                     /locus_tag="PC460_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601909.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(6752..7291)
                     /locus_tag="PC460_003043"
     CDS             complement(6752..7291)
                     /locus_tag="PC460_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2601910.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(7284..7697)
                     /locus_tag="PC460_003044"
     CDS             complement(7284..7697)
                     /locus_tag="PC460_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2601911.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(7733..8854)
                     /locus_tag="PC460_003045"
     CDS             complement(7733..8854)
                     /locus_tag="PC460_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2601912.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            9453..11105
                     /locus_tag="PC460_003046"
     CDS             9453..11105
                     /locus_tag="PC460_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF2601913.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            complement(11488..12414)
                     /locus_tag="PC460_003047"
     CDS             complement(11488..12414)
                     /locus_tag="PC460_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2601914.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         4226 a         2076 c         1406 g         4823 t
ORIGIN      
        1 tttcatttgc tttacttttt catttattta gatttttcat ttgttttagt ttttatttta
       61 tttaaagttt tcacttattt aagtttttat tttccttaaa tttttcattt atttgttaag
      121 tttttatttt ccttaagtct tttatttact ttggcattca ccattccaaa ctatatctct
      181 atatttatca gggtcagtat ctccttctgt acttattaat aatactttag agttctcatc
      241 tagtttcaaa gcttctctta agtcttttaa atcttcactt tcaaggattg aaactaatgc
      301 tccagttgta actgctccag attctccaga gattactttc tcatctcctc ttagagggtt
      361 tcctaatatt ctcatacctt tagcagatac ccagtctggt gctgaaacga atgctgatga
      421 ataatttttt aacacttcaa aacctatagt gtttggttct ccacaagcaa gccctgccat
      481 tatagttggc atatctcctc caacaaatct tggttttcca tctccagctt ctgctgattt
      541 atagtaacaa tcagcctcat cagcctctac tactactgtt attgggcatt catcatatat
      601 tgaagcaaca tacccttgaa ctgcaccagc aagtgaacca actcctgctt gaacaaatac
      661 gtgtgttggt ctatctactc catattcttt taattgttct atagcttctt tagccatagt
      721 tccatatcct tgcattatcc atgctggtat ttcttcatat ccgtcccatg ctgtatcttg
      781 aaccattact ccatggtcat cagcttctgc ataatcatta gctaatctta ctgcatcatc
      841 atagtttaag tctgttatac tagcatctgc accttctcct ttgattttat ttaatctaaa
      901 ttcagaagaa ccttttggca ttaatacaac agatttttga cctaatttat tagctgtcca
      961 agcaactcct ctaccgtgat ttccatctgt agctgtaaag aaagttattt ctcctaattt
     1021 ttcttttatt tctttcgaag tcaatttgtc ataccctaac tcagatatat ctgtatttaa
     1081 tctttgagct aagtatctac ccatagagta agaaccacct aaaactttaa atgcgtttaa
     1141 gccaaatcta taagattcgt ctttaacata tattccgctt acgcctagtg attttgctaa
     1201 gttatctaat ttaactaaag gagtcttttc atattgtgga aagctttcat gaaaagcttt
     1261 agcctttgca atctcttcat cttttaaaaa ttcaattcct ttttcttttt cctctgattt
     1321 aggtaagtta tttaatttcc attttatttc tctcatttat tttatattcc cctttcaaat
     1381 ttttcggtgt cattttaatt ttttatcttt gtgccttata gcaaagcaat taccgtgcca
     1441 atattattta ttctaaaaac aattttttta tgttggaatt acatactttt ataaaatttc
     1501 tttaaagttt ataaattttg agtgtgaaaa ataagattta taaaagaaaa aattctcata
     1561 ttgggaaatt tttatcaata cgagaattat caattcgaga aatcgtttat ttatcttaat
     1621 tcttccttta ttatgctctt attttttata tgtctattgt tttgtcagat atattactac
     1681 ttttattttc tataaatata tggctataac cttcttacgc ataaactcta taaatttata
     1741 tactttatta cccaaatatt ctttattacc caaagcctcc aaaatggctt aaatcttttc
     1801 atagcttctt acagaagttt acatggtcgt ccccccaata agcaaaagtg caatatgtgt
     1861 accattgtgc cctacttgcg actattgagc tgtcgaaatt cgcaacttcg catttatctg
     1921 atgaaatgca ctatacaaaa tattcataac atccacaaca gagttaaagt tacctattca
     1981 tattcactgt aaaaatttct tccacaggaa atgtaatttt aaccaatcct attgagacaa
     2041 actcaccact tatttttttg aactcacgca gtaatacatc acttgcccga aaaaaagcct
     2101 cactttcatc atctccagta agtgctattg tgcaatgggg tacccaacga ttggggtggt
     2161 accattccca accatttgaa tcaaaatctt gtagtacctc atataattct ctctgaaact
     2221 gatacattgc tattcattat aggtgataca aacaccgttt tagtatcatt aaacatgcct
     2281 actgaaccaa tgtaagcagg tattacctta tggttttgtg caaacccctt tagcttgtct
     2341 atgcaacaag cctcatccac atcattgaaa caagccaaag taagatgagg tcttgttttc
     2401 cattctaagt acttcgtact aattttttca tcagcgattt ttttagataa atcaaaaagt
     2461 tgcttttccg tttttttatc ataatatagc tctatcgcat attgcataat aacacctcca
     2521 ccataaagta aaaataccta tctataaaaa cacacaaata cctatcattt taatattata
     2581 ttactactgt tcctcttcca attttctata tagtgtagcc aaacctatgc ctaagtcttt
     2641 tgctatcttc ttcttcgtct ttgtatctct tccatactta ttcaacaact tgtttatgta
     2701 agtcaactct aattctttaa ttgaaagtat cctttcttga ttctctacta tccctccaac
     2761 tatatcatct tcaaatatta tatttacatc cttattttta taaatatttc cattaatatt
     2821 ataatagttt aaaatatcta atggaagcat atctttatat attattccat catctccaac
     2881 caaattcatc atatattcaa ctgcattttc cagttccctt atatttcccg gccaattata
     2941 gttaatcaac atattcatta cattttcttc tattttattt aactgaatac ctaattctaa
     3001 agaatattta gaagcaaact tcatcattat tgcctcaata tcatcctttc tttccctaag
     3061 aggtggcaaa tttataggaa ttacattcaa tctatagtat aaatcatccc taaacttacc
     3121 ttcatttact aatttcaata agtttttatt tgtagcagca atcactctta tatctaaatc
     3181 tataagttta tttgacccaa ttctcgcaaa ttttctctct tgtaaaactc taagtaactt
     3241 tgcttgtaaa ggcattgaca aatctcctat ttcatcaaga aatataacac cttcatttgc
     3301 aagttcaaat ttacctactt tccctccact actagctcca ctaaatgcac cttttatata
     3361 gccaaatagc tcactttcaa ggagattttc tggaatagca gcacagttta tcgcaataaa
     3421 tggcttgttc catctgctac cttcagcatg tatagcccta gcaattagct ctttacccgt
     3481 accactttcc ccagttataa gtacagttga atttgacttt gctaatttct tagttctctc
     3541 ttttactttt aacattgctt ctgaatttcc aattatagac tctatatcac tatttcccca
     3601 gccactattc acttttccat gtcctttatg attgatatat gtcttgtcaa atattattat
     3661 tttgtcgtat tgatttatat atggatatac cggaattatt ttggcattta tattataaat
     3721 tttgttatca atatcaattt ctagtaattc atgtccattt gaggaagatt caacagatac
     3781 tatatttact atattttcta ttatattttt atctatatct aattttttta gagctatatt
     3841 gtttatataa gatattttgc tatttatgtc tagtattata acgccttttt caacattttc
     3901 aatgatttct atcatcatat ccatttttct actgctattt tttgacgctt catgttcttc
     3961 tattttcata gaaattaaat cgcatatatg attgacaaat tttaaataat tatctatttt
     4021 tgcacttatt tctacttttt tagttttatc tgtgctaata gcaccaatta ctcctatagt
     4081 tcgattatta tactttattg gaactgctat tattacttta tttaggcatt tcatgtaatg
     4141 ggaacattct atacaaagct gattatcaca tatatccaat acgactaatt cctgacctgt
     4201 ctgtataaca ttgtcataaa taaagccctc agtaacaacg ctttcattta tctttgattt
     4261 atacagacct gtaccactaa ttcttattaa tctatcgtca actatttcaa tatctacatc
     4321 taacacacta gctactgttt cagtgtattt tatcatttct tgttgaaatt cttttaattt
     4381 acttaacata gtttccccca ttagaattta atttaccaaa ttatcagttt agctatattt
     4441 taacatatag tcactcttta aaatatatat aaaacattac ttatttatat atttgcgtta
     4501 aaacagacaa tattaattta taaaaagttt gttttatata gtaaccttaa ttataatatg
     4561 cagacaataa aataaatcac aatacaataa taaaaaaaag ctttcctcat ataacaaata
     4621 tatttttgac ataaaaatat acattatatg agaaaagctc aatagttaaa tatttagata
     4681 gatttttata tagacttttt taatataaac ttttttaatt gtagactttt tttattagtt
     4741 tttaattttt attagttttg ttattggttt tattgttata atacttataa tactgccatt
     4801 gcttcaattt ctaaaactgc atcttttggt aattgagcaa cttgtacaca tgaccttgct
     4861 ggtggatttt ctttaaaata tgtagcatat acttcattaa tatcattaaa atcctttaaa
     4921 tcttttacga atactgtagt cttaaatatt ttatccatac ttgtaccagc ttgttctaat
     4981 attgctttta cattatctaa agaagctttt gttgcttctt ttgggtcaga tactaactcg
     5041 ccagtcttca tatctattgg aagttgacct gatgtagaaa ctatgtttcc aacttgaatt
     5101 gcttgagaat atggtccaat tgctcctgga gctttctctg tagctattac tacttttgac
     5161 atgacttcac ctcctattat taagttatat ttacaggaaa ataaaatttc ctaaacacca
     5221 atattaatac gaacctttag attaaacttc aaatctaaaa cttcaaataa tcaatactta
     5281 gatgattatg tttatttgtg tcttgttaac ttttattaaa gcaattaacg tgccaaactt
     5341 tatttatata taaaattttg tgaagtcatt caaaatagtt acttctgaat tttttcgtaa
     5401 aaaaacgctc taaaattact tttttatgat gtcaattttt ttctcatatc tagaaattta
     5461 ccaacatgag aattcttgtt ttgagaattt acagattaaa gttatttttt aaccttaaat
     5521 tctgaacctc ccatgactaa agtcacaggg ttcctgcttc atagaatttt gcataatcaa
     5581 gttattacat atgaaagaac tatagttgac aatcccacta ttataaataa taaaaccttt
     5641 acccatacct tatttaccct agttatcata gagcctattg aaaatataac agctaaatat
     5701 aataataacc aaaaaattat caatatctac cactgttgct gaaaatccaa aaaactacct
     5761 tttgatagta ttacttaaca atctaattaa tctcataata aatttacaac ttataaataa
     5821 attgttatat tggccgtatc tcaacaactt tctcctttcc tagttttaaa tcttttatag
     5881 aattgtacca cgtctaagta aattttcata ctattaagtc ttatttctaa taaatcatta
     5941 gctctcattc tatgcccaat aatgtaagat tgcagtaatt ttattgattt taatagtttt
     6001 atttaatata cttatattaa ttattttcat atgaaattat tatttatata gggggatttt
     6061 tttaatatga gcaaaagtga attaacagca gaaacaacag aagaaatgtt agaagtacta
     6121 agtggtaaag attatgatat tgcatgtcat ttacatgaac ttggtaaatc attagattgt
     6181 aaaattgaac caaaaacagg tgctcgttct tacaaaatag tatattcaac taagaaacca
     6241 aaacgtagct tatttactat tgaatgtaat gaaaaaaaat ggagagttaa agcaaatctt
     6301 tttcatctaa atacatataa agatgctgtg gaggaatgct ctaaaactat taaagatagt
     6361 ataactaaaa ctcgtacttg taagaaatgt aattcaaagt gtattggagg ttcttcgttt
     6421 gaattagatg gaaagtctta cctgacttgc ataggaagtg gtcattattt tgcaaatatg
     6481 gaagaaatgg attggaaaaa cctagaaaaa ttaattacta aagaaaataa tattatgcag
     6541 gaatctgtat agttttttaa gggtaattat taaaatatat gagtaacaac agatacttaa
     6601 ataggcataa aaaaataaag ggtataataa ggatatagaa taaaaataaa tgatatagca
     6661 caaagaggct gtctcaaaat acagataagc ctctttttat tgttgaaatg ataagaatat
     6721 attcaatttt atacaatttt atacaaacat actacgccaa cttacccatt tcatatgcct
     6781 gtttcattgc cttacttccc ttaatatcac ctacattcca agcacctaca ccatatacta
     6841 tacctttttc cttagaacca tttagacatg atgtaaatcc tctaaatcct tctattgttc
     6901 tttccatatc agccacattt tcatctgctg ctgtaacgat aaaatagaaa tctttatcac
     6961 aaatttcagt atatctagag caagttctat caatcatagt tttcatttgc gcattcatag
     7021 tataaaaata aactggagtc gctaaaacaa tcacatctgc cccaatcatt ttttcaagaa
     7081 tttctcccat gtcatctgtc tgtgaacaat ttgcttcttt ttcataacaa gcaccacatc
     7141 cagtacaata gttaatatct ttatctttta aaaatatctt ctccacttga tttttagatt
     7201 ctaatacccc ttgtataaac tggtcacaca ataaatcaga atttccacct tttctaggac
     7261 tagctgatac tattaataca tttttactca taagatactg cctccatttt cttatttcta
     7321 ctgctcttta atactttttc tttctcaata ataccttctt catatctatc aatcttaaaa
     7381 tttaatcgtt ccaaagtttt ttgcatttct tctattcttt ttgctagttg gtttctctgc
     7441 tctattaaaa gttcttttct agctttgata gtactgctcc cttgttgaaa catagctaca
     7501 tactcaatta atgtctcaat agaaagacct gcacctctca tacactttat gaaattaacc
     7561 cactcacaat cttcttctgt aaaagaccta attccacttt tatttctatt tacaggtggt
     7621 attaaaccaa ttcgttcata atatcgtaaa gtatccgctg aaagctcgta ttttttacta
     7681 acttctgaaa tcgtcataca tatcttctcc tcttgatatt gtatagattg aattatttct
     7741 caaaggtttc tgctacattc tttaaagcat ctataatttt aatgtgttga ggacagcttt
     7801 cttcacattg tttacattca atacaatctg aggcctttcc ataagtttta gtataattat
     7861 tatagtagac catctgtgtt gaaaatccag tattaagagc ttgtttttct gcattgtaca
     7921 acgcaaaata gtttggaata gcgatatttt ttggacagcc ttctacacaa tactgacagg
     7981 cagtacaagg aattaaaatt gattcgttga taatctctac tgcttcatct ataatattat
     8041 attcctcttg tacgaatggt ttgaaatttt gcatatatcc tgtattgtct aataattgtt
     8101 ccatatttga catcccactt aagaccatca taacattatc tttacttgct gcaaaacgta
     8161 ttgcccaaga tgaaattgac atatctggat tgtagtcatt caataatttc tctgcttttt
     8221 ctggtatctt tgcaagtgta cctcctttta ctggctccat aacaattaca ggcttattat
     8281 gcttctctgc cacctcgtag cattttctag attgtatgct ttcattatcc caatctaaat
     8341 aattaatctg taattgcacg aagtctactt ctggatgctc actcaagact ttatccaata
     8401 attctgcact atcatgaaaa gaaaaaccaa tattctttat ttttccttct tttttctttt
     8461 cttcaataaa cttaaagcta tcaaattttt tagctacttc ataatgtgac acgcctatat
     8521 tgtgtaaaag atagtagtca aaatagtcta caccacattt ttctaattgc tcattaaaaa
     8581 tggtttcttg ctcttctttt ttctttaaag ccattaatgg aagttttgta gctatagtaa
     8641 aactctctct tttgtgtctt tttactagag attctctaag tgctatctca ctttttccca
     8701 tgtgatacac ataggccgta tcaaaatatg taaatcccct ttctaaaaaa gtgtctacca
     8761 ttttatttaa atgttccata tcaatagttg taacatcatt ttcatctttt attggtaaac
     8821 gcattaatcc aaatcctaat ttttttgcct ccattgtttt acttccccct ttaaaacttg
     8881 tttgtttttt ctctctactt taactattct tacaagtata taattcttgt ataattatac
     8941 tttactactt ggagttaact tcaagtcaat agattttttt atttttaaga caaatattat
     9001 aaaaatacag ttctttaata ttaatccatt acatgtttct ttcaccctcc aattctcaaa
     9061 cttcacaaac tccaacatgt aaagaaatat atactttaag aaattcagtc cattaatatt
     9121 actacaaata ctttttagat atgtttaaaa agaaatttca cctaaaatat ttataaaaga
     9181 tttagaaata ggtcttgtgt ctattttaaa atatataaaa cagtgataaa gacttattta
     9241 taagataaaa aatgaatagt gtataccctt tttaaaaagt tttttccttt ttatatttgt
     9301 gatacaatgt taaatataaa aaggaaaatt ttgtattaaa gaaatcttat ttgttgattt
     9361 tgtaacttca aactcagtat caatttgatt ttaagattat attttattaa gtatgctaaa
     9421 taacaaattt ttaaagattg tgaggtaaaa atatgaataa gaaaaattta gctatattaa
     9481 tatcaggtat gatgtttttt tctagttctt cagcaatttt tgcagacaat actacaaagc
     9541 aagaaagatt aataggtaaa acaagatatg aaacagcagt agaagtaagt aaattgggat
     9601 gggttcaatc aaaaacagcc attatagtta atggtaattc tattcaaagt gcgctttgtg
     9661 caaatccatt tgctaagcta aagaatgcac ctatactttt agtaaacaac aacagtattg
     9721 aaaattcaac taaggctgag cttaaaaggc ttggtgtaga taatgtgtac attgtagata
     9781 gtggaaatag catttcttct aaagtagaaa atgaaataaa atcacttaac ataaaaataa
     9841 ataaaatagt aggaaacaat atatatgaaa tgtctacaaa tgtacttaaa gaaatagata
     9901 agataaaaaa gatagaaaat gttgcagttg taaatggact aaagggacta tctgatgcag
     9961 ttagtatagc ttctccagca gcaatacata atatgcctat aatattagta tcagatgaaa
    10021 acagcaatga tagtagtata aatttcttaa acaataggtc tataaaaaaa tcttacatag
    10081 ttggaggaaa taagatttta tcagacaata cagtaaagaa atatccaaac agagaaagaa
    10141 tcagtggttc agatagaaac aatactaatg caaaaattat tgataaattt tataaagata
    10201 aaaaaataaa taatgtattt gtagctaaag atggtattaa ccaagaaaat gcattagtag
    10261 attcattatc aataggagta ctagctgcta aagaaaattc tcctataatg atatctaatg
    10321 gtagccttgt aaatagtcaa aaagaattta ttaaaaataa agatatagat gaaataacac
    10381 aagtaggagg aggatataat caaaaagcat ttgaagatat tttaaaacta aaaaatgtga
    10441 acataagaaa taaaactaaa aaaatatctt taatacaggc taaagaaata ttaaaacaag
    10501 ctcttccagg aagcaatatt ttagtttgtt attatgatga agatgataat gaatatgact
    10561 gcaaagttat aaaggataat atcctatatg agatagaaat tgatgcctac tctggtaaaa
    10621 taatagaaat tgaaaaagat gatgacttaa atgataataa tttaggtaca caaaatatat
    10681 cattggataa agctaagtct atagcattag atgctattcc taatggtaat ttaatctatt
    10741 gtaaatatga tgctgaagat aacgaatatg aggctaaagt aataaaaaat aagaaaacat
    10801 atgaattaga gataagtgca tttgatggaa gaattgtaga tattgacatg gacaatgatt
    10861 ctgatgatga ctttgatgat aatgataaga ataactctaa tactaatgac aacagttcta
    10921 ttattagtgc tgaaaaagct aaatcaataa tgctagataa agtgcctggt gggaaaatag
    10981 taaaatttaa gtatgatgaa gatgacaaag aatatgaagg tgaaataata aaaggaaaaa
    11041 ctgaatatga gataacaatt aatgcaatta ctggtaaaat attagaattt gaaatagatg
    11101 attaataaaa aatataaata gatgattaat aaaaaatata tattctatta attattacaa
    11161 gcaataaagc cataaatatt gaaattttca atatttatgg ctttatttta tttttttaat
    11221 ttaaatccac attatgccat tatatatatc tatgcactct tgtaaaatgg gttatatttc
    11281 tccagtatat taaaaaaagt gagtgtctca aaataaatct tctaactcat aaagcactct
    11341 cctttgtatt aaatcaaata tattttcatc atttaaacaa ttaagaagag acttctctct
    11401 attttgagac aactccattt taatacacta tttatacata aacatatttt aaacaactaa
    11461 aaagcaacta atatcttaat aaatatctca atacatatct tcataacaca tatcaccata
    11521 attacaagca gataaagaac tctcctcatt taaatacgac tcaaattcat acccaaacag
    11581 aattgttggt tctatatata tttccatatc tgtatatctc catcttctta cctttgcctc
    11641 tataaccttg taaaaatctt ctaaactata cccttctttt aatcttttat ctataagtgc
    11701 cttagttata ctcaaatcag ctttaaaatt ttttcctgat tcactattta attttcttat
    11761 aataatttca tgtatgcttt ctggattttt gtaagtagaa tcttcagtca aatcttcatc
    11821 cgaattttca aaggtatttt tatttaaatt ctttttattt aaattatttt ttaataattc
    11881 ttttttcttt gtctcatttt ctgtttcata cccagtatga attatcaaat cataatcttt
    11941 gtctatactc tcttgattta tacggctgta tatgttggta ttactgttct taaacaactc
    12001 atttctatgt ttatatttca ttgttttttg gttattgtta atagttgcat caatgtattt
    12061 ctctatattt ttgaaaatat caccattctt tgttacaatt tctgcattac tactttcaga
    12121 acaaacataa ctatatacag aacaacaatt tccctttact cccctactta ttaatctcaa
    12181 tatacctaaa tctataaatt catttacaag tctacttatt gtagatatag atacatcaag
    12241 gtcagatgca gctttcctta ctgtcatcat aaattgtcct cttggcagag ttccttttaa
    12301 tctttgtata ttttcttttc tcattacata gtgattaaac cttattctat ttacatctct
    12361 tttttcatta aaatataata ctgctgaatt taattgataa aaactattac tcatattccc
    12421 actcctccaa tttaaatata taatttgttg taaagttttt agatttttaa ataacttatt
    12481 aaatgattat ttttattaaa aagtattctt tgatattttc attttaaagt t
//
LOCUS       DAMJWU010000104        12452 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMJWU010000104 DAMJWU010000000
VERSION     DAMJWU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.108"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     repeat_region   589..1012
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=589..617
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1176..1349
                     /locus_tag="PC460_003048"
     CDS             1176..1349
                     /locus_tag="PC460_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDF2601915.1"
                     /translation="MSSKMGRPKVDNPKNIDVKVRFDENTHKKLLDYCEKENLTRTEA
                     IRKGVDLLLEEDK"
     gene            1473..2300
                     /locus_tag="PC460_003049"
     CDS             1473..2300
                     /locus_tag="PC460_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HDF2601916.1"
                     /translation="MNNELMNFENNELGIKIRTIKYEDGSIGINAEDTAVGFGWCQTQ
                     NKNGKQYVSIRWETINKYCKEFGFLNLLGKDEYIPESLFYLLGMKAKNEIAVKFQTWL
                     AVDVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSK
                     LLTAKTLYKKAGIDLPIEIEEEESFFDTKQIASKLKIYSKSNKPAQLAVCEIIKKIDL
                     EENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPAKIAGEKKNYHVVYKIE"
     gene            2363..2539
                     /locus_tag="PC460_003050"
     CDS             2363..2539
                     /locus_tag="PC460_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601917.1"
                     /translation="MCENLLNEYNLKTDEDVEYFVKFATLLYKLKQDNEEKFQEYAEI
                     LRGILREQQERENK"
     gene            complement(2886..3089)
                     /locus_tag="PC460_003051"
     CDS             complement(2886..3089)
                     /locus_tag="PC460_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601918.1"
                     /translation="MYFNIDKLLESKGRTRYWLAKEVGIAYPNMMKLANNETSSIKLD
                     LFEKLCLVLECTPNELVTLSSKE"
     gene            3333..3719
                     /locus_tag="PC460_003052"
                     /pseudo
     CDS             3333..3719
                     /locus_tag="PC460_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726857.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            3783..4136
                     /locus_tag="PC460_003053"
     CDS             3783..4136
                     /locus_tag="PC460_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601919.1"
                     /translation="MKLLVTKTFFERAGIELPLEVEEKEHFYDTKQIAKKLGLYTMSN
                     KPAFMAVKQIIRKLDIKEDEMKTVLETNGNWTGTVTKYSNKVTEKVKVWLKENNNPIS
                     IKGDKKNYYILYKNN"
     repeat_region   4568..5519
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4568..4596
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
     gene            5649..6164
                     /locus_tag="PC460_003054"
     CDS             5649..6164
                     /locus_tag="PC460_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2601920.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREDE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     gene            6229..8622
                     /locus_tag="PC460_003055"
     CDS             6229..8622
                     /locus_tag="PC460_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2601921.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIDSITKVADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMAITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVSNVTLGAILIINGLGWVTNATLNVANVFAQNWSIIAPIIYG
                     VIAAIAIYKGVLLASTIATTVASFVNSLYAVAAYKSCAALAAQEFAIFGKISAQTMEA
                     LVTAQATAAQWGFNAALLSCPIFWIIAGIIAFVVVVFVAVAAVNKFSGTSLTVLGAIV
                     GAVFAAVAFIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DAVNNAGNALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNKWVGDAPEKIKLDRMGYKDIGAEFQKGNALGTKWQNAITDKFKDTFDINK
                     MLEDAKKKLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMIVSAEGIV"
     gene            8638..9327
                     /locus_tag="PC460_003056"
     CDS             8638..9327
                     /locus_tag="PC460_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2601922.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKIVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGAFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDAENGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKKVPKY
                     TIYKGQVLRLV"
     gene            9320..11212
                     /locus_tag="PC460_003057"
     CDS             9320..11212
                     /locus_tag="PC460_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2601923.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPAVIDGITWEIERRGSPSK
                     LEFTIAMDDILEFCEGNSVRIYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLNLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDVLLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            11226..11486
                     /locus_tag="PC460_003058"
     CDS             11226..11486
                     /locus_tag="PC460_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2601924.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            11491..11910
                     /locus_tag="PC460_003059"
     CDS             11491..11910
                     /locus_tag="PC460_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2601925.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFENIKGKVQCKFSVHTKYGNIKAEKVVSV"
     gene            11923..>12452
                     /locus_tag="PC460_003060"
     CDS             11923..>12452
                     /locus_tag="PC460_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2601926.1"
                     /translation="MTFENIIKRMLDSVPNTLDKREGSIIYNALAPVAVELTETYIAM
                     DELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGEYNYI
                     AIERISEKTYKMKCETAGPIFELGKLIPIEYIDGLETAELTEILINGEDEESEDSLRQ
                     RYYDSLNSQSFGGNIQ"
BASE COUNT         4882 a         1346 c         2321 g         3903 t
ORIGIN      
        1 taatttataa aatatatata aataagtagg tatttattta cttgaatttc attgtttata
       61 taaaaaaaat ggaaaaatat gtaataatta tatgttataa taattgtagc aaggataata
      121 atcgaaagtg cgaagggtga ttattttcat attaaacgcc aaattccaaa taaggaagga
      181 ggtgaaatta tatgataggt tttttattaa gcatactagc tggtgttata tcagcttata
      241 tttatgacaa aataaaaaat cacccagacg ccaataaggg tgatttaaaa aaataattct
      301 ttaaatcaat tttgatggaa atagctactc ttgtataaag taaattattt ccttgctttt
      361 attataccac aaattggtac agatattcaa aaataatatt tttatgatat aataaaaatg
      421 tagagatttt gcagtgagca atatttgcga taaattgaag tttaacaatt ggaatacaag
      481 gtattgaggg tgtgtgataa atgttatcaa ttgcactact catggttcac tgcaaatttg
      541 agagagatgc gtatgtgtag gtattggaaa tgccaagttt attttggggt tttagattaa
      601 ctatatggaa tgtaaattaa cctttttatt gttgttgtta ttcttattgt tattcgtttt
      661 agattaacta tatggaatgt aaatgttgta agaagtatca ttctattttt taatctttct
      721 gttttagatt aactatatgg aatgtaaata gtacatataa tgagtcttta acatcagtta
      781 tgaaaggttt tagattaact atatggaatg taaatgattg tactttagcg tctgcactag
      841 ctttgtctat cgttttagat taactatatg gaatgtaaat tattttacag atgaacaatt
      901 acagttactt cttgaatgtt ttatattaac taagtggtat gtaaataatg gaagcaagat
      961 aatttttaaa ggtatggata attgttttat attaactatg tggattcaaa attaaataga
     1021 aaaaagaaag cacttactta aatagtaggt gcttttgttt tgctcaaaat tggtcggttg
     1081 agtaaaataa ttagaaaaaa ttgggataag ttattgactt tttggaacac aaataatata
     1141 attatattat ggaacacaaa aagtgaggtg aaacaatgag ttctaaaatg ggaagaccaa
     1201 aagttgataa tcctaaaaat attgatgtca aagtaagatt tgatgaaaat actcataaaa
     1261 aattattaga ttattgtgag aaagaaaatc ttactagaac agaagcaata agaaaaggag
     1321 tagacttact tttggaggaa gataagtaaa aaagtagccc aacgccgacc aaagcatact
     1381 ggactactta acctagagtt atctctatat gaaatattct atcatgtaaa gataactctt
     1441 tcaagataat aaatcgaaag ggtgattttt gtatgaataa tgaactgatg aattttgaaa
     1501 ataatgaatt aggaataaaa ataagaacta ttaaatatga agatggaagt ataggaatta
     1561 atgcagagga tacagctgta gggtttggat ggtgtcagac acagaataaa aatggtaagc
     1621 aatatgtatc aattagatgg gaaactatta ataaatactg taaagaattt ggtttcctca
     1681 acttgttggg gaaagatgag tatatacctg aatcattatt ttatctttta ggtatgaaag
     1741 ctaaaaatga aatagcagta aaatttcaaa catggttagc agtagatgta ctaccatcaa
     1801 taagacaaac tggtgcatac ataactaaca atgctaatcc cgaaaaacta agagaaaaag
     1861 caagtgagat tgaaaagtta caattagcct ataacagcac atctatgtta aaagaattgc
     1921 tagatggtgc aggatttgac aataaatcca aactattaac agctaaaaca ttatataaaa
     1981 aggcaggcat tgatttacct atagaaattg aagaagagga atctttcttt gacacaaaac
     2041 aaatagcatc taaactgaaa atatattcta agagtaataa accagcacag ttggctgttt
     2101 gtgaaattat taaaaagatt gatttagaag aaaacgaagt caaaggcgtt tgggaaacta
     2161 atggttcttg gactggtact gtaaataaat atacaaagag tgtaatagat aaggttagaa
     2221 attggataga ggaaaataat agacctgcta agattgcagg tgaaaagaag aattatcatg
     2281 tggtttataa gattgagtaa atttatcagt tgtattaaat aatttagttt agtttatttt
     2341 agttttgagg gggattaata caatgtgtga gaatttactt aatgaatata atttaaaaac
     2401 tgatgaagat gtagaatact ttgtaaagtt tgctacatta ttatataaat taaaacagga
     2461 caatgaagaa aaatttcaag agtatgcaga gatattgaga ggtattctta gggaacaaca
     2521 agagagagaa aataagtaaa ataatataga taaagcactt gaatattaca tagtttcaag
     2581 tgctttgttt ggtataaaat ggtataatag agataagagt tatattaact atgtggtatg
     2641 taaagaatgt ataaggcatc ttctcaaatt ctgcgttggt cattttatat taactatgtg
     2701 gtatgtaaag tgcgttaact gcaataatgc tgaactcatt tcttgatttt atattaacta
     2761 agtggtatgt aaatgtttca acatttgaca tgtgctgtgg agtaatgttt atgttttata
     2821 ttaactatgt ggattcaaaa ttaaatagaa aaaagaagca cttacttaaa tagtaggtgc
     2881 ttttgttatt ctttgctaga taaagtaaca agttcattag gtgtacattc aagaacaaga
     2941 caaagctttt cgaataaatc taattttata gaacttgttt cattgttagc taatttcatc
     3001 atgttaggat aagctatacc tacttcttta gctaaccaat atctagttcg acctttggat
     3061 tcaagtaatt tatcaatgtt aaaatacatt ttttcacctc ttaagtatat tatacaacaa
     3121 ttatataatg tttgcaatat ataatatgca aacaatataa ttatataatg ttgacattat
     3181 ataatgtgtg cgatataata tacttaagga ataaaaaata agagccactc gcccctacca
     3241 agtttgagta actcttattg acatatacta catatacatt aattatagta tatgtcattc
     3301 cttaaaaaaa atcaattaag gagtgtgttt ttatgaacaa tttagtacta attaacaatc
     3361 aagaagtgca agttaaggaa tttaataatc aaagagtagt tacatttaaa gaaattgaca
     3421 gagtacatga aagagtagaa ggaacagcag gtagaaattt tagagaaaat aagaagcact
     3481 ttataaaaaa tgaggactat ttttatttag aaggaaaaga attatcaaca attaaacaga
     3541 caacgaattt cgttggcagt aatgcaagag aattaatctt attaactgaa acaggttatc
     3601 taatgttagt aaaatctttt acagatgatt tagcttggaa agtacaaaga caattggtaa
     3661 atagttattt tagagttaaa gaagaaaaga aagaaatgaa agcattagaa aaattagaaa
     3721 cagttaatga gtcaattagg ttaataactc caatttttga tgatttaaac attgataaaa
     3781 gtatgaaact tcttgtaact aagacttttt ttgaaagagc aggtattgaa ttgcctcttg
     3841 aagttgaaga aaaagaacat ttttatgata caaagcagat tgcaaaaaaa ttaggacttt
     3901 acacaatgtc taataaacca gcatttatgg cagttaagca aattataaga aagcttgata
     3961 ttaaagaaga tgaaatgaaa acagtattag aaacgaatgg caattggact ggtactgtaa
     4021 caaaatattc taataaggtt acagaaaagg tcaaagtgtg gttgaaagaa aataataatc
     4081 ctatctcaat caaaggagac aagaaaaact actatatatt gtataaaaat aattagattt
     4141 tagtttttgg gggggtaata caatgtatga gaatttactt gatagtatag atattgaaaa
     4201 gagaaaagaa gaatttagaa ttaaactttt gaaaataaga gaaacagata tagatatata
     4261 tatataataa gatagaaaga atagtatata aactttctga gaaaaaatta gagaaaaata
     4321 attaaataaa tagataaagc acttggatat tatgttgttt caagtgcttt atttggtata
     4381 aaatggtata ataaaaatat aggaattttg cagtgttcga tttttctact taagtatagt
     4441 ttaacaattg gaatacaagg cattgagaag gtttgataag tgttatcaat tgcactatta
     4501 cccgctcact gcaaatttga gaaagttata tgtgtgtaag tcttggaaat acacaattta
     4561 ttttggggtt ttatattaac tatgtggtat gtaaatcttt ttaaaaaagc acgtcaatta
     4621 caatactaaa attgttttat attaactatg tggtatgtaa atttgaaaac tgcatgttct
     4681 ttaacaaaag catctaattg ttttatatta actatgtggt atgtaaatgt attactccca
     4741 attatgaaca taggatatag tctttgtttt atattaacta tgtggtatgt aaattatagt
     4801 ttgccatatg ttcctggttc gtatgattgg ggttttatat taactatgtg gtatgtaaat
     4861 aaatttaata ttactggaaa aggagaaatg gatatcggtt ttatattaac tatgtggtat
     4921 gtaaattttg ttataagaat atcgccacct tccttttctg gtgttttata ttaactatgt
     4981 ggtatgtaaa tttttctgca tcttctacta tgttatattc ttctaatggt tttatattaa
     5041 ctatgtggta tgtaaatgca catttaactt caagtcttag agtattatct gcaagtttta
     5101 tattaactat gtggtatgta aatttcgttc aacttttatc ttagcttctc ctgctacttg
     5161 ttttatatta actatgtggt atgtaaatgt actagaaaat gcactacctg cattcattcc
     5221 tatacggttt tatattaact atgtggtatg taaatgtgct atttccaact tttttttcaa
     5281 gttctgaata ctgttttata ttaactatgt ggtatgtaaa ttagttagtc ccatatcgtt
     5341 atggtactgc attaacgcgt tttatattaa ctaagtggta tgtaaatagt gtaaaaggca
     5401 ttttttcaaa ttgtgcattg gtttggttta tattaactaa gtggtatgta aaggctaatg
     5461 tttgagtatt taaaagcatc tgagcaaaca attttatatt aactatgtgg acttaaaatt
     5521 aaaaataatt caaaaacact tacaaatgag taagtgtttt tttaatgaaa ggaggtgata
     5581 ataatgtaaa aattttacgt atatagtata atagtgctat aaaataagta ttatgtgagg
     5641 tgattgttgt gttttgttca aattgtggtt atgagataac tggtgcaggc aaattttgct
     5701 caaattgcgg aacagctaca ttagcagata aagttaacaa tgatgattta tttataaatg
     5761 ttcatggaaa agaattaaat ctgactaata tttataaaga aactaaagga gataaaatct
     5821 tagcaattga tattgcaatg aagttactag ggctggacat aaaagagtgt aaaaatatta
     5881 tatatccagc ttttaaagaa ttaagtgaaa aaataaatat cgaagaggaa aaagagatat
     5941 tgagggaaga tgagtataaa caaactaatg tacttgaaga tgatgttgct cgttgcccta
     6001 ggtgtggctc tgtttcattg tctgctcata agaaaggttt tggcatagga aaagctgtag
     6061 caggggctac tatagcagga ggcattggtt tagtagctgg aaatttagga gcaaagaaag
     6121 ttagagttac atgtttgagc tgtggtaaac agttttgggc ataaataata aacacttact
     6181 aatgtaggtg tttttttata tggaaattta tgaaaggaga gtgaggaaat ggctacaata
     6241 caaacttcaa taaagatttt cgacggaatg acaccagcat ttcgtaatat gactaattct
     6301 attaatacaa caattaatag tttagagaga ttgcaaggca gattgaataa tccactcaat
     6361 gcaggtaata tacaagcttc tcaacaaagt ttgaataaca tagaaagtat tctcacaagg
     6421 atagaacaga aaattggaag aaatacaaat gaacaggaaa actttaataa taaaataaga
     6481 caaggtagtg aagcaggttc tctattagtg tctaaattaa aaagtattgc tgggatatac
     6541 attggaataa aaggaataga tagtattaca aaagtagcag atacaattgc aagtacaaaa
     6601 gcacgtttaa atctaatgaa tgatggctta cagacaacag accagcttaa taaaatgatt
     6661 tatttgtcag cccaaagtgc aagagctagt tatgcagata cagcagcaca gattgctaaa
     6721 cttggaatac ttgcaggaga tgcttttgga agttcagcag aggtgataaa gtttacagaa
     6781 cttatgaata aagcttttgt aattggagga acatcagcaa atgaagctag tgcagcaatg
     6841 tatcaattaa cacaagccat gggtgcagga aaacttcaag gtgatgagtt ccgttccata
     6901 atggaaaatg cacctttatt agccactaaa atagctgatg caatgggaaa aactaaagac
     6961 caattgaagg aattatcaag tagcggagca ataacagcag atgttataag aaatgcactg
     7021 tttaaagctt ctgatgagat agaaaagaaa tttgcaagta tggcaatcac tttttctcaa
     7081 gctctcacaa tgatgaaaaa tgatgcttat atgatatttt ctgagactct cagtaagata
     7141 agtggagctt tgcaaagtgt acgttttagt gagattgttg tatctatgcg gaatgttatg
     7201 attgcaatat cttcaaacat ttatgataca ttaaatatta taaaaaatat attaaatagt
     7261 gatttttttt cagattttgt tagcaatgtt acattaggag ctatactaat tatcaatgga
     7321 ctgggatggg ttactaatgc aacactaaat gttgctaatg tctttgctca aaattggtcg
     7381 attattgctc ctattattta tggagtgata gcagcaattg cgatatataa aggagtatta
     7441 cttgcaagta caattgccac tacggtagca tcttttgtaa attcattgta tgcagtagca
     7501 gcgtataaat cttgtgcagc tttagcagca caagaatttg cgatatttgg aaaaatatct
     7561 gcacaaacga tggaagcttt ggtaacagca caagcaacag cagcacaatg gggttttaat
     7621 gcagcattat tatcctgtcc aattttttgg ataatagcag gtattatagc atttgtagta
     7681 gtagtttttg ttgcagtagc agcagtaaac aaattctcag gaacaagtct gactgtacta
     7741 ggagcaattg taggtgcagt atttgcagca gtagcattta tacaaaacat aatgatatgg
     7801 ctatttaata gatgcgtaga tgtaaatgaa gggattgcaa atggatggaa tcaatgtgta
     7861 tatctaatga aacaagctat tgcaaaaggt gtaatcttta taattgagaa aatggcatca
     7921 ttaaatgatg ctgtaaataa tgctggaaat gcacttggga aagctttcat agatggggca
     7981 aatatcgcaa tacgaggtgt aaacaaatta attgacctaa taaataaaat accagggata
     8041 aatattggta aagttggaga agcaacattt acgcctgtca aggcagataa tagttacatc
     8101 aaacaacaga ttgacagttt aaataaatgg gtaggagatg caccagaaaa aataaaattg
     8161 gatagaatgg gatataaaga tattggagca gaatttcaaa aaggaaatgc acttggaact
     8221 aaatggcaaa atgctataac tgataaattt aaagatactt ttgacattaa taagatgcta
     8281 gaagatgcaa agaaaaaatt aggattagac gatttgtgga ataaacaaaa tcctttaaac
     8341 aaccttggtg gatttggtgg agatttagga aaaaatgtaa aagacacagc aggaaacaca
     8401 gccaaaatgg ctaaaacaat ggataaaagt caagaagacc ttaaatactt aagagacatt
     8461 gcagaacaag aaacaataaa cagattcaca ggagtaaaca taaaaattga tatgaacaat
     8521 actaacaata taagtaaaga cacggatgtg gatggtatag taaatgtttt aacagaaaaa
     8581 ctgaatgatg ctatgattgt atcagcagaa ggaatagttt agaaaggagg gatataaatg
     8641 gcttatgact tttatttaga tggagtacaa ttaccaatac ctccgccaaa gttagagatt
     8701 aaagttacaa ataaaaacaa gatagttgat ttgataaata ctggagaagt aaacatacta
     8761 aaaaaagaag gattatctga aataagtttt gaagcagaat ttacacataa taagttgcca
     8821 ttctatcgtg gagcttttag ggatgttcaa ttctttttaa gtaaactgga actattaaaa
     8881 actgattgta agccatttca atttattgta tcgagggaat taggtaataa agtactattt
     8941 aacactaata taaaagtatc tcttgaagag tatgctattt cagaagatgc agaaaatggt
     9001 tcagatacaa aagttgcaat aaagttaaag caatatagag attactcaac taaaaagtta
     9061 gttcttgccc ctcctaaaaa tgagactggt agacctaatg taaagataga gccaaaacga
     9121 gttgattcag tcaatgccac aaacactaaa acatatacag taaaagcagg ggatagcctt
     9181 tggtcaattt gtcagaagca acttggtaat ggttcgttat acaagaaagt atatgagtta
     9241 aataaaacaa tgatggataa agctaacaag ggtaaaaaag tacctaaata taccatctat
     9301 aaagggcagg tgttgaggct tgtctgatga tttagttctg gcgaatgata gagatataag
     9361 gctagttatt gcacattggg aagatttcta cgaacctgct gtcattgatg gtatcacatg
     9421 ggagatagaa agaagaggtt caccttcaaa gttagagttt actatagcaa tggatgatat
     9481 attagagttt tgcgaaggta attctgtaag gatatattat aagggaattg gcatatttta
     9541 tggatatata tttcagaaga aaagagataa agaaaatcac attaaaatag ttgcttatga
     9601 ccagttgaga tattttaaaa ataaagatac ttatgtatat agtaataaaa cagcaagtga
     9661 acttgtaaag atgttggcta aagattttaa tttaaaatac aatgtcatag aagatacaaa
     9721 atataaaata tcgagagttg aagaaaataa aacactcttt gatatggtct taactgcact
     9781 agatgatact ctaagagaga aaaaagaaat gtatgtgtta tatgatgatt ttggaagaat
     9841 aacattaaag aatgttgcat caatgaaatt agatactgtt atgaacaatg atgtaataga
     9901 agactttgac tataattcat caatagatag tgatacttac acaaagatta aacttgtgag
     9961 agataatgaa gaaacaggaa aaagagatgt gtatattgct caagactcaa cgcatatgag
    10021 gagttgggga atacttcaaa tgtttgatac agtagacaaa aacatgagtg aagcagagat
    10081 aaaacaaaag tgtgatatac ttctaaaact atataataag aaaactaagt cattaagttt
    10141 aaaaaatgta cttggagata ttagagtgag agcaggttgt ttagtacctg tttttttaaa
    10201 tctaggagat attaaacttc aaaattatat gctagttgag aaagtaaaac atacttttga
    10261 aaataacagt cattttatgg atttgactct tgttgatgga gatgaatttg cttcttattc
    10321 ttcaagctca tattcaagtg gaaatactaa caataaggat gaaaagaaaa atggtcctgc
    10381 acaaagtatt acgaaaaaaa atacaggtaa aaaagttcct gctatattta ctgcatatta
    10441 tccagggaac aatgcaatgg aaggcggaaa aacagattgc aatggaaagc cacttgatgt
    10501 aaaatcaaga actgttgctg gtccaatgaa tcgagaagga gttaagaaaa cttggtatac
    10561 tgatgatttt ctaaagaaac atccagtttt tgaatatgga gataaagtaa aaattatact
    10621 tcctggtact gcctatgaca acaaagtata tacagttaaa gataatggag gaagaatata
    10681 tgttgaaaca aacggaacat atcatataga tgtactatta gctaatgcta gtgaatgtaa
    10741 aaaatttggt agaaagaatg gctatataat tataggtgga gatgaagaac aaacatatca
    10801 agttgaaggt aataaccaaa gtagtacaaa taataactct aaagaagata aattaattag
    10861 tatagcaaaa agtaaactgg gttgtaatta tgtgtatgga gcagaaggtc ctaataattt
    10921 tgattgcagt gggtttactc aatggtgtta taaacaaata ggtataaaaa ttcctcgtac
    10981 tgcttctgca caaagtaaag caggaaaagc agtagattta aaagatagaa gcaagtggaa
    11041 agcaggagac ttattatgta gaattggagg aggaagtagt aatcatgttg taatgtatat
    11101 tggaaacaat caaataattc attcaccaca aacaggagat gtggtaaaaa tagagtctgt
    11161 taattcttat agaaaaggaa aagcatacac acatgtgaga agatttatat aagtgaggtg
    11221 gcaatatgag ccaagattta ttacagataa taaaaaaagc tgcaatggat gcagtagaaa
    11281 caagcaaccc aatgagggtt gtatttggaa caatagaaag tattagtcct ctaagagtta
    11341 agatagaaca aaaactatct attggtgaaa tttttctaat acaaacagat acatttaaaa
    11401 gatatacaga taaaaaaata ggagataaat tagtcttaat tcgtatgcaa ggagggcaac
    11461 aatacttgat tttagatagg atgtgataaa gtgttaccaa gtgataattt agattatgat
    11521 attgaagatg tatcaataat taattttgat gtaagacaag agccaagtaa gacatttaaa
    11581 cttcatatag aaaagtctaa gattgatggt atttgtgatg atgtagaagc attaaaacag
    11641 accatctttt tgattttaaa cacagagaga taccaacatc taatatatag ttggaattat
    11701 ggagtcgagt tgaacgacct tattggagag cctatatcct ttgtaatacc agagttagag
    11761 agaagaatca aagaagcact aattcaagat gatagggttg aaaatgtaga taattttgag
    11821 tttgaaaata taaagggtaa agtacaatgt aaattttcag ttcatacaaa atatggaaat
    11881 ataaaagcag agaaggtggt gagtgtataa ttgtttgagt taatgacatt tgaaaatata
    11941 attaaaagaa tgttagatag tgtacctaac acacttgata agagagaagg ttctataata
    12001 tacaatgcct tggcaccagt tgcagtggag cttacagaaa catatattgc tatggatgaa
    12061 ttactagacc aaacattcgt agatactgct agttattact atttagagaa gagatgtaaa
    12121 gagcgaggaa ttacaccttt accagccact aatacaattg ctaaaggagt ttttaacata
    12181 gatattccac ttgattctag gtttaatcta ggagaataca attatattgc aattgagaga
    12241 atatctgaaa aaacatataa aatgaaatgt gaaactgctg gacctatatt tgagttagga
    12301 aaactaatac ctattgaata tatagatggt cttgaaactg ctgaactaac tgaaatcttg
    12361 ataaatggag aggatgaaga gtcagaagat agtttaagac aaagatatta tgatagccta
    12421 aattcacaga gctttggtgg gaatatacaa aa
//
LOCUS       DAMJWU010000105        11839 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMJWU010000105 DAMJWU010000000
VERSION     DAMJWU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.109"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            197..1237
                     /locus_tag="PC460_003061"
     CDS             197..1237
                     /locus_tag="PC460_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2601927.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1593..2915
                     /locus_tag="PC460_003062"
     CDS             1593..2915
                     /locus_tag="PC460_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2601928.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSIITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3212..4573
                     /locus_tag="PC460_003063"
     CDS             3212..4573
                     /locus_tag="PC460_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2601929.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTAIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4646..6040
                     /locus_tag="PC460_003064"
     CDS             4646..6040
                     /locus_tag="PC460_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2601930.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6102..6572
                     /locus_tag="PC460_003065"
     CDS             6102..6572
                     /locus_tag="PC460_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF2601931.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6575..8791
                     /locus_tag="PC460_003066"
     CDS             6575..8791
                     /locus_tag="PC460_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2601932.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8874..9545
                     /locus_tag="PC460_003067"
     CDS             8874..9545
                     /locus_tag="PC460_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2601933.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9908..11122
                     /gene="dpaL"
                     /locus_tag="PC460_003068"
     CDS             9908..11122
                     /gene="dpaL"
                     /locus_tag="PC460_003068"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2601934.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11249..>11839
                     /locus_tag="PC460_003069"
     CDS             11249..>11839
                     /locus_tag="PC460_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2601935.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4474 a         1364 c         2400 g         3601 t
ORIGIN      
        1 ttgcttattt aatagagcat ttttgtaaaa tgaagaaagt atatctatca aattaaaact
       61 aagtagatat actttttatt aagttttgtt ttaaaataat attattaaat tataactaag
      121 cttttctgtt atgatataat taaatagata atgtgaatga ttattatata gaatacttat
      181 ttggagttga tattttatgg acaaacacag tgattcagta atgtatggtg gaatggaagt
      241 atataatatt tggggaacaa caacagtaga ctccataatt ggagagtctg atgtaatgcg
      301 tgctcttaag aatagaattt gtaaaattgg aaatagtgat tctactgtag ctataactgg
      361 agaaagtggc actggaaaag aattaatagc aagagccatt catctcgcag gaaacagagg
      421 gaataaaaca ttcgttgcta taaactgtgc tgctatacca gaacctttac tagaaagtga
      481 actttttgga tatacaaaag gagcttttag tggagcagat tcaaaaggta aaattggtaa
      541 gtttgaattg gccaatggag gggttgtatt tttagatgag ataggagata tgccaattca
      601 actacaatca aagttattga gggtattaca ggaaaagaag tttacgagaa taggttcaaa
      661 tgaactaatt gatatagatg ttagaatcat atcagctact aatagagatt tatttgagtt
      721 ggttaaggaa aataaattta gagaagactt atactataga ttgaatgtaa tacctttaga
      781 agtgcctcca cttagagaaa gaggagacga tatacaaata ttaataaaca attttataga
      841 caaatattcg agaaagatga agaaaaacgt gtaccatata gagccagatg tggaacaaat
      901 gttgcttaag tatccttggc caggaaatat tagagagttg gaaaatacta tagaactggc
      961 attaaacctt cttgaggaag atggaataat acataaagga ataataaata gaaagatact
     1021 tgaatatttt aagttaaata atataaatat aaagttactt gaggaaagtg actatgagat
     1081 aagtatggaa caagacatac ttacacttga agagtttgaa agagcctata taaagaaagt
     1141 gttaaagttt tacggaaatg atactaagtc taaaaaactg gctgccaaaa aacttggaat
     1201 atcactagct acattgtaca gaaaaataga tgtataataa ttaggctttt tattctcaat
     1261 tctaaaattt gaaattataa aattgataaa aaacacttgg aaaactattt tctaagtgtt
     1321 tttttgtgta atagcatttt attatccaaa aataaaaaaa atttaaaaga atttaaaaaa
     1381 ataaatgctt taataaatta tcaaatacta acaaatataa taaaaattat atatgtaaaa
     1441 caattatgtt aaaaaattgg cattataatt gcttcatatt aagttgagat gacaaaataa
     1501 aaaaagaaag gatgttgtgt gagataataa taactgtcat taaaaaatag ttgggagtga
     1561 taaccttgag taaatatcaa aagaatgttt ctatgtttga tattgatgga aggccaccaa
     1621 ttttaaagtg tttgccacta tcaatgcaac atatactagc aatgatagtt ggaactgtta
     1681 cagtacctat agtcgtagga ggagctgtgg gtgttagccc tacacaaata acacttttag
     1741 tgcaatatgc attaataata gctggtatat caacacttat acaactatat ccaataggaa
     1801 atattggttc aagattgcct ataatttttg gagttggttt tacttatgta ccaacacttg
     1861 tagcagtcgg aggaaattat ggattagcaa gcattttggg tgcacaatta atagggggag
     1921 tggctacagt attaataggg atattcataa aaaagataag aaaatttttt ccgaatatag
     1981 ttgcaggaac agtggttttt acaattggat tatctttgta tcctatagca ataaattata
     2041 tggcaggtgg tgtaaatagt cctacttatg cctctatgca aaactgggca gtagcggcta
     2101 taacattagt ggtagttata ggtcttagtc aatttgcaaa aggatatgca aaattggcag
     2161 caatattttt gggaattata gttggatatt gtgtatcttt agttctgggt ataataaatt
     2221 ttgaccctat tagagaggca acttggtttg cgatacctaa accgcttgaa tttgggatgg
     2281 aatttgatat gcctgtaata atctcagtag taattgtaag tataatcaat gctgttcaag
     2341 caattggaga cttatcagct actacaatgg gaggcatgga taggaatatc acagataagg
     2401 aattatcaag tggagtaata ggaagtggaa tttgtactat gataggagct ttgtttggag
     2461 gtttaccacc atcatcatat agccaaaacg tggggatggt agctatgaac aaagtaataa
     2521 gtagatttgt gcttggaata gctggtatat ttatgttatt gtcggggttt gtgcctaaat
     2581 ttggtgcatt aatgactact ataccatatt ctgttttagg aggagctaca atatctatat
     2641 ttagtataat aacaatgaca ggtataaagt taattattac agaagagttg tcatcaagaa
     2701 atattaccat tgttggtttg gcaattgcct taggcatggg aataacatca gtcccagcag
     2761 cccaagaggc atttccttca tttgtaaagt tagtatttgg agaatctcca atagtaattg
     2821 caacattggt tgcattcgtt ttaaatctaa taataccaaa taaaagtcta gaggatgagg
     2881 ctaaagaaag agctgttatg gaagagacta attaagatat aataatttaa aagaattgtt
     2941 taaattattt agtatgagat tgttagatta tttttgatag ctattcattt taataaaaaa
     3001 tttaacaaat aaatatatac caggcaatta atgttcattt aagttgaaaa tcggggaggc
     3061 aatatttaga aatttgttaa ttttttataa aaatagctca gtttagctgt gtttatcggt
     3121 tgagcaataa attcatggtc ataaaaatat ttatagaaaa taattataaa aatatttagt
     3181 caagatattt atgtatggga ggatttattt tatgaaaaca cgtaatactt caaaatatga
     3241 tgtcgatggg ataccaccac taaaggaatc aatacctttg gccttacaac atctgttagc
     3301 aatgatagta ggaaatatgg tacctgcgat attaatagca aacgtggtag gacttaacca
     3361 ggggcaagca acaatgttga ttcagggttc tatgttagca gcaggtttag caacgttttt
     3421 acagttatat ccaatacctt tatttaaagg atttaagtta ggttcaagat tacctgtaat
     3481 gatgggaatg agttatgtat ttttaggagc ttgtctttca gtagcagcta aagatggttt
     3541 agcagctcta tttggagcac agattgctgc tggggtaatc gtattctttg taggatttgg
     3601 agttaagaaa attagacata tattcactcc aatagtttct ggaactataa tagcatgtat
     3661 gggacttgga ctattcgcaa cagctataaa gaatttagct ggaggagaag gcacacaaac
     3721 atttggaagc ccaataaact tcacagtagg agttatagtt gcttttgtaa taataatgat
     3781 aaataaatac ggtaaaggtt tagtaaaaaa ttcttcaata ttaataggaa tattagtagg
     3841 ttatgctata tctttagttt taggattggt tgatttctca gccgttcaag gagcagctat
     3901 agtatcttta ccaacaccag cagcttttgg attagaattt agaccagagt taatagtaat
     3961 gtttactatt atttacataa taggtatagc tgatatgatg ggtgcctgta caatagctac
     4021 agtaggagca atggatagag aggtaactga cgaagaatta gcatcagttg tattaggaaa
     4081 ttctataact tctataattt catcactatt tgcagctctt ccaactggag tatttagcca
     4141 aaatacagca attgtatcta tgaacaaagt tacaagtaga tttgtaatag cactaggggc
     4201 acttgtatta ttactagcag ggatatctcc agcattgggt gcaataatga caactatacc
     4261 atcttgtgtt gttggtggag caactttagt agttttctca tcaatagcaa tgtctggttt
     4321 ctctattatg agtatggatg gattcactga agaaaacaat cttatagcag gtgtatctat
     4381 agcaacaagt atgggtctta caactgctcc acaagtttta gaccaattcc cagaaacaat
     4441 tggaactgtc cttggagggt caagtatagt ttctggagca ataatagcta tattacttca
     4501 aacattattt aaattaaagt cacgtaaaag tgctgaaaac gttacttcaa atcttgaaga
     4561 aaatattgga taaatgtatt agatataata tgagaaaacc atatggtaaa atgttgtaat
     4621 gaaagtattc aaagggggat ttaagatgat tgatatacta ataaaaaatg caataattgt
     4681 tacagttaat aaagacagag atgttatatt tgatggagca atagctattc aaggtgacag
     4741 aatcttggac ataggaaata cagttgatat cgagcctaag tatcttgatg ctaaaaaagt
     4801 tatagatgct aatggaaaag taatattccc tggctttata aatactcata atcacctgtt
     4861 ccaagtgtta ttaaaaggtt taggtgatga tatggcactt catgagtggt taaatacaat
     4921 gatgtttcca tcagcaaaat tcttaacaga acaagatact tatgatgctg caatgcttgg
     4981 atgtatggaa ggtttaaaaa gtggtataac tacaatggtt gattatatgc atactcacaa
     5041 tagaccagga cttacagatg gaatagtaaa ggcatataaa gatttaggca taagaggggt
     5101 tgtaggtaga ggttgtatag atttaggtat tcacaaagag cttatagaag atgtggaaac
     5161 ggttgaaaaa gatttgagaa gggtattcga aaaatatcat aattcagaaa atggaagaat
     5221 aaaaatctgt gtagctcctt cttcaatgtg ggctatatct gaagaaatgg gtaagatgct
     5281 ttggaatata gttaaagaat atgattcata ttttacagtt catatatctg aaacagaatt
     5341 tgcaagaaca gcaacaaagg atatacatgg agaaatagat ataaaattac ttgaaaaatg
     5401 gggtatagta ggtccagaag ttgtagcagt acattgtgta tgtataactg atgaagacat
     5461 ggaaatgctt aaaaagtatg acataaaagt ttctcataat gtagcaagta atatgtatct
     5521 tgcttctggt gttgcacctg tgccagaaat gttaaaaaaa ggtataaatg tgagtttagg
     5581 tcttgacggt gcagcaagta ataacgcaca agatatggta gagcttatga aactaacagc
     5641 tttacaacac aaagtaaata attgtgaccc tctggcaatt tctgcagaga aagttcttga
     5701 aatggctaca attgagggag caagaactct tagaatggaa gatgaaatag gttctttgga
     5761 gattgggaaa aaagctgacc tagtaatatt taactcaatg ctttctccaa aatcaatacc
     5821 tcttcacaat cctgtatcaa ctttggtata ctcagctagt atgcacaatg tagagagtgt
     5881 tttagttgat ggaaatgtaa tacttgaaaa tggaaaagta actacaattg aaagtgaaga
     5941 acaagtgttg cttaatgcac aacatgcagc agaagaatta tgtaagagag ctaatgtaac
     6001 taatagaatg gaaggacata agtggaatag cttatactaa tattaaaata agattgtaat
     6061 aatattctat tacaacatta gtttatctgt aggaggagaa aatggaaaaa ataaaattaa
     6121 atattaatga ttacatgtat gaaatagaag ttgaaaagaa tgaaactctt ttgcatgtac
     6181 ttcgtgaaaa attaaatcta actggagcga aacaaggttg taatagtggc agttgtggag
     6241 catgtaaagt tttgatagat ggagttgaca tgaaatcttg taaacttctt gcaaaaaact
     6301 gcgaaggtaa agttatatac actatagaaa gttttgctaa tggagaaaat cttcatccaa
     6361 tacaagaagc ttttatagaa gttggagctg tacaatgtgg atattgtact cctggtatga
     6421 ttataacaac acaagctctt ttaaatagaa atgcatctcc taatgaagat gaaattagag
     6481 aagcttttaa agaaaatctc tgtagatgca caggatatgt aaagattata gaagctgtaa
     6541 aattatctgc agctagatta gggggtagat aattatggaa aaggtaatag gaaaatcata
     6601 ccctctaaaa gatgcagctt taaaagttac tggtcaaatg aaatacgtgg ctgatatgaa
     6661 accacaaaat gtactttacg ctaagatgct tttaagccct gtagcacatg caaaaataaa
     6721 aagtatagat acaagtgaag ctgagaaagt agtaggtgtt agagctatag ctacttatct
     6781 caatacatca caaagaaaat acaatggttc attaagattt tatgagcaca aaatacctga
     6841 aaatgaagtt atattttcag atacagttag atttgttggt gaccgtgttg cagcagtagc
     6901 tgcagagact ccagaaatag cagagaaagc tgtaaaactt ataaaagttg aatatgaaga
     6961 attgccagct gtatttacta tacaagatgc aataaaagaa gatgctatct gcatacatgg
     7021 agattcaaat atagtttctc aaaacaacat aaatggggga gatgtagaaa aaggcttcaa
     7081 ggaatgtgac tatatatttg aagatgaata ctctactcca gcaatacatc atagtgcaat
     7141 agagactcat agtgtaattg cacactatga ttataatgat aaattaactg tacatacacc
     7201 ttgccaaaat acatttggat ttagaataat actatctcaa atttttggga tgtcattaaa
     7261 taaagtaaga gttataagac ctgctattgg aggctcattt ggtggaaaat tagaacttac
     7321 aattgaacca gtagcagccg cattatcaaa aatgacaaga agaccagtta aaatggttct
     7381 tacaagaaag gaatctatga tatcaacaag aactcgtcat gggtcttttg taaaattaaa
     7441 aactggtgta aataaagatg gtgaagtaat agcacaagac ataaaaatat atacaaatac
     7501 aggagcatat gcatcaagtg ctttaaatgt tataggagca cttagtcata aagtatttaa
     7561 agtttataaa ataccaaata taaagtttac tgggatgcct gtatatacaa atacaccaat
     7621 agcaggagct atgagaggct atgggtcacc tcaaatattt atggctcaac aagctcaatt
     7681 tgcgaaaata gcaaaggaaa taggtatgga tttagtcgat tttcaaaata aaaatgcagt
     7741 agagccagat gatgtcgata taatcttcca cggttctctt ggaaatccta gagtattgga
     7801 ttgtatagag caaggtaaga agatgtttaa gtgggatgaa aagaaaaaac ttccaaaaga
     7861 agaaggaaaa tatttaagag gtataggtat ggcaataggt gcacatggta atggagtttt
     7921 tggtgcacat agagatgttg ttacattgac tcttaaatta aatgaagatg gtacgttgac
     7981 actgctaaca ggagctcatg atatgggtaa tggtgttgta actatgcaaa caatgatggt
     8041 agctgaagtt ttaggaataa cacctgataa agttgataca tttgaaactg atacggatgc
     8101 atgtacattc aaccttggtg actatgcaag tagaggggta tttgtagaag gtggaggagc
     8161 taaaaagaca gcagagaaat tgaaaaatct tatattagaa gaagcagaaa aattacttga
     8221 aacatcaaaa gaagatttat atctagacaa tggatgtgta attagtaaac ttgatgaaaa
     8281 tgtaaaagca tcactttctg atgtcgcagt atatagtcaa agtaaatcat taagagagtt
     8341 aactgttgtt gaagattatt cttcaccagc tggacgtact tcttatggtg tacattttgc
     8401 tgaagtttta gtagataaag aaactaagga tataaaagta gttgattttg tagctgtaca
     8461 tgatgttggt agagttataa atcctctaaa tttagaagga caattagaag gtgggattca
     8521 tatgggtcta ggatacgcat tgagtgaaga attggcattt gatgaacaag gtagacctaa
     8581 agctactgat tttaaaacat ataaattatt tagaactgta gatatgccaa aatgtcaagt
     8641 tgcatttgta gatggttatg aaaaagctgg gccttttggt ggtaagagta ttggagaatg
     8701 tgccgttgta ccagttgcgc cagctgttgc aaatgcagta tataatgcaa ctgatgtaga
     8761 gatacattca ttaccaataa aattaaaata gtataaatta aagcagaaat aacaatttat
     8821 tgtatttgta aatagataat aaaaatagat ttatttggtg aggagatatt attatggcaa
     8881 agatattagg tatttgtgga agtccacgta aaggagcaac agaatatgca gtgttagaag
     8941 ctttgaaaga agcagaaact atacctggta tagaaactga gtattggtca gttagaggga
     9001 aaaaaataag tccttgtgta cattgtgatg catgtataag aaaagaaact atgtgtatta
     9061 taaaagatga tatccaagaa ttagagcaaa aaatattgga ggcagatgga attatagtag
     9121 gttctccagt ttatgatatg aatataactg ctcaacttac agcagtattt aatagacttc
     9181 gtccaatgta tttagttcat ccaggaaaac ttcaaaataa ggttggtgca gctataacta
     9241 ctggtggaac tagatatggt ggccaagaat tgacaaaact tcctataata aatttcttcc
     9301 taatgcatga aatgttagtg tctggaggtt taggtggatg ctatataggt ggtacaatat
     9361 ggagtaaaga tggaaaagca aaaggtgccc aagaggatga gacaggtatg gacacagtaa
     9421 aaagacttgg aaaaggtgca gcagaggcag ttatggtttc taagtttggt cttgaaaaat
     9481 ggaatgtcgt aaaagaagaa ttagatgtta aaaaagatga aaaatcacct cttagagacc
     9541 actaaagatt tattatatta ttgaatatat acaaatagtg atgtaataga ttattgcatc
     9601 actattttat gttttaattt agattttata gattttatat taaaaaatat agaaacaaag
     9661 tgttttgaga tttattttta aattttaaaa atgagaatta aaaagtgaga agatagaaaa
     9721 ggttttttag ttattaaatt ctacatacat tgtatgaact aaagatagaa aagaaaaaat
     9781 aataaaagca ttgcaatttc tatactaaag agtcatagaa aaatatctag tatatattta
     9841 gtgttggcat gttaattgct tatatattaa agtataaaaa tttaacaata atttggaggt
     9901 attttagatg ttaaaagaaa ttaaatggaa agtaaataat ttgccaaagg gcgataagga
     9961 aaattgtatc aaatttttaa atgaagaaga aataactaag gttagaaatt ttcacaaaag
    10021 ctttcctcaa tataaagaaa ctccattagc aaacctagaa ggtttagcaa aaaaattagg
    10081 tgttgcagga gtatatgtaa aagatgaatc ttatagattt ggattaaatg catttaaagt
    10141 attaggtggt tcttattcta tgggtagata cttagctcaa agattagata cagatatatc
    10201 tgagttaggg tatgacaaat taacttcaaa agaaataaaa gaaaaattag gagaaataac
    10261 tttctttaca gctacagatg gaaatcacgg tagaggagtt gcttggacag ctaataaatt
    10321 aggtcaaaaa tctgttgtat taatgccaaa aggttcttct gaatttagat taaataaaat
    10381 taaaggagaa ggtgcagatg ctagtataac agacttaaac tatgatgatg cagtaagatt
    10441 agctaatgat tatgcagaag ctgatgacca tggagtaatg gttcaagata cagcatggga
    10501 cggatatgaa gaaataccag cgtggataat gcaaggatat ggaactatgg ctcaagaagc
    10561 tatagagcaa ttaaaagaat atggagtaga tagaccaaca cacgtatttg ttcaagcagg
    10621 agttggttca cttgctggtg cagttcaagg gtatgttgct tcaatatatg atgaatgccc
    10681 aataacagta gtagtagagg ctgatgaagc tgattgttac tataaatcag cagaagctgg
    10741 agatggaaaa ccaagatttg ttggaggaga tatgccaact ataatggcag ggcttgcttg
    10801 tggagagcca aatactatag gatttgaagt attaaaaaat catgcagcag catttgtttc
    10861 agcaccagac tgggtttctg ctaagggtat gagaacatta ggtaatcctt taaatggtga
    10921 tgaaaaggta atttctggag aatctggagc agttacaact ggtcttttag tagcagctat
    10981 ggaaagagaa gatttagcag atttaagaaa agatttaaaa ttagatgaaa attcaagaat
    11041 acttttaata agcacagaag gagatactga ccctgacaaa tatagaagta tagtttggga
    11101 tggagaatat ccaagtatat aaggaattac taaaatgcaa ttattagata ttggtttaca
    11161 tatttagaag atatataagt tctaaaattt agaaaatata taagtatata aaatacatac
    11221 attataaaaa atctaggggg ataatgaaat gttaaacgaa caaagaaaac aagaagtaac
    11281 tgaaatatgc caaaaattaa ttcaaaatcc tagttcttct ggaaatgaag aaggtgttgt
    11341 aaaagcaata gaagagtctt ttgaaaaatt aggttttgat agttggtcaa gagatagata
    11401 tggaaatata gtaggatgta taaaaggtaa caagccaggt aagaaaatat tatttgatgg
    11461 acatatagat acagttccag ttccagatgc aagtaaatgg agtgttcctc cttttgaagg
    11521 aaaaatagtt gatggaaaga tatatggtag aggaacttct gacatgaaag gtcaatatac
    11581 tgcaatgatg tctgctgtag cgtattttgc taaagacact aatagagatt ttgctggaga
    11641 actatatgta gcaggtgttg ttcatgaaga aatatttgaa ggtgtttcgg ctagagagat
    11701 aagtaaggct gttcaacctg actatgttgt aataggtgag tcttcagagt taaacttaaa
    11761 gattggtcag agaggtagag gagaaatagt agtagaaact tttggtaaac cagctcactc
    11821 tgctaatcca gaaaaaggt
//
LOCUS       DAMJWU010000106        11691 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMJWU010000106 DAMJWU010000000
VERSION     DAMJWU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.110"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            148..648
                     /locus_tag="PC460_003070"
     CDS             148..648
                     /locus_tag="PC460_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2601936.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(898..1677)
                     /locus_tag="PC460_003071"
     CDS             complement(898..1677)
                     /locus_tag="PC460_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601937.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1674..2153)
                     /locus_tag="PC460_003072"
     CDS             complement(1674..2153)
                     /locus_tag="PC460_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2601938.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            3139..4146
                     /locus_tag="PC460_003073"
     CDS             3139..4146
                     /locus_tag="PC460_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2601939.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            4184..5611
                     /locus_tag="PC460_003074"
     CDS             4184..5611
                     /locus_tag="PC460_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF2601940.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTKKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SAGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQMYMGVEE"
     gene            5753..7018
                     /locus_tag="PC460_003075"
     CDS             5753..7018
                     /locus_tag="PC460_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2601941.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTRIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            7060..8658
                     /locus_tag="PC460_003076"
     CDS             7060..8658
                     /locus_tag="PC460_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2601942.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            8693..9715
                     /locus_tag="PC460_003077"
     CDS             8693..9715
                     /locus_tag="PC460_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2601943.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(10103..11506)
                     /locus_tag="PC460_003078"
     CDS             complement(10103..11506)
                     /locus_tag="PC460_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2601944.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
BASE COUNT         4096 a         1414 c         2002 g         4179 t
ORIGIN      
        1 tacaagagat agatatattg gttttaattt tatagaaata tacaaagata ttttaagcta
       61 atatatagta atttatatag cattataaaa tagaatcata taaaataagt taactacaat
      121 caaaaattaa aaaggggaaa tgttaagttg atagaaggca taaaatttat aaaggcagaa
      181 gaaaagtata tttattcgta ttggcaaact tttgataaaa tagcaaaaga gaggaaatat
      241 ctagctatgg atgaagcctt tccttttgaa gaaacagttg aatttataaa aaatattata
      301 aataaaaact taccacaact gtttataatt gatttagaat cagataactg tattggttgg
      361 tgtgacgttt tacctaaaac tgaaaaagtt ggttatttag gcatgggtat tcttaaagag
      421 tatagggaaa aaggtatcgg aagtaatctt ttaaaacaaa ttattgattt atcaaaagaa
      481 tatgggtatg aaaaaattga gcttgatgta tttaaaagta atagtagagc tattcatgtt
      541 tataaatctt tagggtttgt agaggtaaat acaatatcta gtggttttac atggaatgat
      601 agacctgtaa aagaagaagt aattcaaatg gagcttacac ttatataaaa taaagttaaa
      661 gtctttaaaa ttccatatac acttgtataa aataaagtta aagcccttaa aattccatat
      721 aaaagaaata tttttaaggg ctttttaata atgcttcttt tgaaggtagc taaatatcta
      781 aaattttata tattaaattt aaagaaaata taaagatgga agatacaacc atgtttacta
      841 ataaaaaaat taaagtcata aataataaac tcataaacaa cttattatta cttatgttta
      901 ttttgcatct attacttcat gagccatttt aactaagtca ttcatagata aatttgagtc
      961 ttgaactgta aaagaatagt aaactccatc ttgtttccat gtcaaaaact taaatttatt
     1021 aacttcaact ttgtctgatt cattatttgg ccctacacta aatgtgtatt tgcctgattt
     1081 ttcatcaatt ttatcctgtt ctgtcatttt ataaccttct ggtacaaact tttgtgcgaa
     1141 tgcttcataa gaaatattaa tatctttgta agtatctaca acttttagat tgtcagaact
     1201 cttcccaagt acattatttg ctgtgtcaag agatatttca tcattgccat ttttatattc
     1261 aaaactaagt gcttttgatt ttcctaaaac attattttct ttgtcaaatg cttttttatt
     1321 tacagtatat gctcctttaa atttatatcc attactaaat tcagaaacta tattaggggt
     1381 aaagttaaag tcttttttta cttgttctgt agttgggata gatgtatagg tagccttagt
     1441 acttgatttt ccaaccaaac tatatatatg tcctactgca agggctgttg tgctcaatat
     1501 caaagtaatg gctaaagttg caactaagcc ttttctaaat ggtaatttgt gtgttttatt
     1561 attaatcatt ttataatttt cctctctttc tggtaaacta tttagtgttt tgcttaaact
     1621 attatgaaaa ctttctggaa cttctggaaa cgaattattt aatatatttt tattcatatg
     1681 attacctccg tgttttctaa tttagttttt aataatcttc ttcctcttga taatctactt
     1741 tttacagttc cttgaggtat atctaatatt tccttaactt catctactga ataaccttca
     1801 atatagtaaa ggactattgg aattttaatt tttttaggta gactttgaat agcagtatac
     1861 aaatcgctat agtcatcttt ttcatcaatt tttttgtttt tgaagacatc ttcaaatgat
     1921 agagatttta atcgtttgcg tcttaatgaa ttacattcat ttatgactat tctaatcagc
     1981 catgttttaa agtattgttc ttccttaaga gaatccaact tttcatatgc ttttaaaata
     2041 gcattgttga cggcatcttc acagtcttgg tcgtttccta gaatagactt agatactcga
     2101 tacaaagtct gttctgattc aagtatattg tttgtaaatg tagttttatc caactaagtt
     2161 ccttcccttc tatgcaaatc gtagcgctac ttttttgtct ttcacttatt agataaaatt
     2221 ttactataga tggttcactt tttttatgat tatttgataa atttaagttt gaaagttttg
     2281 cctattctat acaaatattc tataatatat tttttctagt attttcccct catatataaa
     2341 gccatgtaga tatattaaac tttagaagcc atttatatat ttagttttat agtaagtctt
     2401 tatattgagt aattagaaaa tagtaagtat ttaatgttga gtagttagaa taacaatgct
     2461 tttggattat ttacttcatg tacagattta aaataagttc tttaaatagc aactttttga
     2521 aaaaacgcaa aaatatttta tgttgtctga ttatatttat agatgtgaaa caatattgta
     2581 tagagtaaaa gtatatagaa gatgattaca tagagtttat acttgaattt tacaaatata
     2641 attatcaaaa tactgtcaaa gagaaaaata gttttgatga acgtttttat caaattgcta
     2701 cagagacagg gctaagtgct aatactgtat ataaagtatt caataaaata ataaatagtg
     2761 gaaaaactga atttacatca aaggaattag ctactatatg tggtgtaagt attagaacta
     2821 tggacaggat tatacttaga ttgtgtgatt ctggttattg tgaggtagtg agtgaacagc
     2881 ttatgagcaa gtatggtaga cccagtagaa tattacgttt taaacctttt ctgttgtttt
     2941 aaatagatat aagtcaaaaa ttaaattttt atgataacat tttctttaca attttttaga
     3001 tatatgttaa tatttagaaa aatgcatatg tattcataaa ataaacttaa aacagctaaa
     3061 aaatcattaa aaaggaaatt gtatgcatga aatgagaaag ttaatttata acttaaataa
     3121 atctgggagg agataattat gggaaaatat caggattcaa aaaaagttgt aagaaattat
     3181 tttgaagcat tagaaaatgc tactggggat gaggttgaaa atgtcttaaa gcaacatatg
     3241 aaatcagatt ataactggaa aggtgtgtat ccatttcgtg aacaagaagg cactgaaaat
     3301 gtagcagatg tattttggaa gcctttaaaa aaatcattga gtaatatgca aagacgacaa
     3361 gatattttca ttgcaggaac aaatgagatt gacaatagtg aatgggtaat gagtatgggg
     3421 cattttatgg gactttttga taacgattgg cttggcatta aacatactcg taaaatgatt
     3481 agtttaagat atgcagaatt taactgtgtt caagatggta aaattgctaa aactggatta
     3541 tttgtagata taattgggtt tatgattcaa gcaggagtta atccattgcc accacaaaca
     3601 ggttcatatt ttgtatatcc agggccaatt gaccataatg gattactttt tgaagatgca
     3661 gatgaagttg aaggagtaaa aactttagca ttagtaaata aaatggtaga tgatttatct
     3721 gaattaaatg aaagtggtgc tatgggatgc ccaccagaaa tacttgaaaa atcttgggct
     3781 aaagatatga tttggtatgg tccaggtgga attggtgctt cttatactat acctagatat
     3841 caagagcaac accagctacc atttagaaat aatttaaaag gaaagacttt caatgggcat
     3901 gtatgtagat ttgcagaagg taattttgct tgtttctttg gatggcctaa tctgactaat
     3961 gtaccatgtg gaggtttttt aggattacca ggtggagagg ttaaagcaga tatgcaagtt
     4021 gttgatgttt attatcgtaa gggcgataaa cttcaagaaa actgggtgtt aattgatatt
     4081 ccttattggt tgaaacaaca agggcttgat attcttgaga gaactaaaag tatttttaat
     4141 gcttagttat gcatacgtat tctaaaataa agggggatat aaagtggcat ctaccaacaa
     4201 aaataatgca acaagtaaag aagaagaaaa agaagtttct acaagtgggc ttgaatcatg
     4261 tacggttaaa gaaaacaaag ctaaaatgaa tcaaactaat tttagtaata taaatgaaaa
     4321 tcaaacaaat gagaataaaa cgaacacata taaaagaaat atagataaag gaaatataga
     4381 taaagggaat atagataaaa caaacacaaa taaatccgac ataaataaag ttaacataga
     4441 tacagtggat acagataaat taaatacaaa tactcttact aaaaaaaata aaaaagttat
     4501 atttaagggg gaagataata tgtcatctac aaaaatttac tatggagatt cattagaagt
     4561 aacgcctgtt ggagtaatgg attacaatga tttttcaaaa cagacaaaaa gggaacagga
     4621 aatacctgga tttgattcta agtatagaga ttttgtagat tatattatga agattacaca
     4681 taatatctgg gaagaaaaag gtataggtgt tatttatgat acatatcaca ataatgttac
     4741 aatgcattgt ggctcttcaa atttagttgg tattaaagat gttatttcca atactcttca
     4801 aacattacat gcgtttcctg atagaagatt aattggtcaa aatgtaatat ggtctaactt
     4861 tggtgctgat ggttttttat cttctcaccg tgttttgtct acagcaacta acttaggtga
     4921 tagtaatttt ggaccagcaa caggtagaaa aataaacttt agaactgtaa ttgattgtgc
     4981 cacaactaac aatagaatcc acgaagaatg gttggttaga gataatttat ggattgttac
     5041 tcaattagga ttaaaccctc aggaagtagc aaagagtatg gcaaaggcaa gtgaatctaa
     5101 agtgcttagt ttacaatcaa cttatggtat ctgtgaatct atggatggtc aatttatgcc
     5161 aactaagtat caagcaaaag atgattctgc tggcgaaatg atgcttgaaa tgacaagccg
     5221 tatttataat tataaatata taaatgaagt taaaaaatac tatcatgata atgcagtagt
     5281 acattttata tgtgataaag atttaaatgg ttatgatgag attcaaggaa tgattgtaag
     5341 tttacttgca tcaattccaa atggaagtta tgaagtggaa agagtaactt gtaatcaaag
     5401 agaaaaaaat gaaggttatg atgtatctgt aagatggaga ttacgtggaa taaatgaagg
     5461 tataggattt tttggacagc cttctggaaa gcatatagaa gtaatgggaa tcaatcatta
     5521 tcatatacta caaggtaaag taaaggagga gtggataact tttgatggta tggatgtatt
     5581 aaaacaaatg tatatgggag tagaagaata aagctgttta gagtataaat agttttgaaa
     5641 tatataggga agtattgctt tataaaattt aagtggggaa tgtatatgaa aaatagatgt
     5701 ttaaactttt agcaaaatta tgactaaatt taaaaggggg aattataaaa atatgaaaac
     5761 aacggttgaa atagaaaatg atatcatatt aggaaaagac tattcaaacc taaaaagagg
     5821 aattgcattt ttttcaattg ctttaatata ctttttttat tgctacaatt ttatggtggg
     5881 tacttttatt aaaccaacaa tgatttatgc tttagcagat gggggatttg gtttttcatt
     5941 aaaacaaaca gaagaaatat ttgcagttat gtcttttggt acaattccag gtacatttat
     6001 ttttggggtg atttctacaa aaattgggaa gaagaggact ttaattagcg ttgctttact
     6061 gattggattg acaacattta taccaatgtt atctcctaca aatattatgt tatggaaaat
     6121 agcacgtttg tgtacaggag tagtgttggg aggcgtattt ggaacagcta tgcctttagt
     6181 tgcagatatg tttccaagta aatatcgtgg taagttagct gctatattaa ctgctttatt
     6241 ttcattagca atgatttttg gtgggaaagt atatggcgtt ttaggtgatg caaattggca
     6301 aatattgatg tatactgcga ttataccacc tatagttgga gctatcttag caattttctt
     6361 tgtgccagat gatttggagt atacaaagga attagttaaa aagggaaaag aaactggtga
     6421 aaagattagc tatataagta tgtataaggg aaaatactta tggattggat tgggaacgat
     6481 tcttttatca ggtgcaaatt ttacagcata ttcagcattt tcaaataatg caactacata
     6541 cctagtgaca agaataggaa tgtcagcagc agtggctggg tctatatata gtttacaagg
     6601 aattggtcaa ttagtaggat atttattctg gggttcaatt gcagataaat ttggaagaaa
     6661 aatacctttt ataggtatgg cattagcagc agtatttgtt tttatatata caaaattagg
     6721 tggagataat acaacaacat tctatatgat atcagtatta ctaggtgttg ctgttggata
     6781 ttctggagca tggggagcat actacacaga gttgttccca agtaaatatc gttcgttgtc
     6841 atctggtatg tcatttaatg gaggaaggat tatatctacg tttgcaatac ctgctatagc
     6901 tggtacagct tctggtactt taggtatgat gactatcttt tacatatcaa tcgcagtctt
     6961 tgtagcaggt gctattgtat ggttattctt accggagacg ttaaataata aaaaataaaa
     7021 tactaggaat ttattttgtt acttagaaag gggtggctta tgatttcatc aattgatgta
     7081 tgtattataa taggatattt gctattaatg ttagttattg gttattattc aggaaaagat
     7141 aataaaaatc aagaagatta tttcttggca ggtaggtcaa tgccatggat tccaattgct
     7201 ttatcagttg cagctactat gattagtgca aatggcttta taggtggtcc tggatgggca
     7261 tatgtagatg gaatgtatcc agtaatggtt aatattacag tacctttagc aatatttttt
     7321 gcactgagca ttaccacccc agttatctat aaattaaaaa taacctctat atatgaatat
     7381 atgggacttc gtttaggaaa ttattcaaga ggattaacta ttattcaatt ttttattaat
     7441 tcattgattc aagctagttc tatggtatat attccagtgc ttattattca aatgattact
     7501 ggctggtcac tttatgcttt agtcccaata gttgtacttg tatcaattat atatactcta
     7561 ttaggtggga ttaaggcagt tatatggact gatagtattc aaatgatagt agtaataagt
     7621 gctgtattta tagtaattat tgtagcctta aaaggaatga atctaagttt ctttgataca
     7681 ttacaaattg cacaacagag tggaaaatta aatacatttg atttcagtag ggatatttca
     7741 gtgacaaata ctttttgggc tactttaatt ggtggaactg taatgtggat aagatacttt
     7801 tgttttgacc aatctcaagt acaaagagtt ttaacatcta aatctttaaa atgtgcaaaa
     7861 aattcttttg tagttagtgc atttgttatg aatttagttt actactttat gctattggta
     7921 ggagtaattt tatttgtttt ttatggggga aggacatttg aaacttcaaa tgaaataatg
     7981 attactttta ttttaaatga acttccagta ggagccattg gaattattat tgcaggtgtg
     8041 tttgcagcag caatgtctag tatcgactct atattaaaca gtatgacaac tgtttttacg
     8101 aaggatattt atgagtttta tttcaaaaaa gacaacaaag aatcatcatt aaaagttaca
     8161 atgattataa ctgtagttat aggagttgtt atgacaggtt ttattattat gggatttgga
     8221 ggaagcatca aatcaattct tgaccttgtt ggtaactata tctcatactt tgctggacca
     8281 gctacaggtg catttgtact agcaatgttt acatataaag ctaatgataa aggagtatct
     8341 attggtttta ttgtagggtt agttttagga ttttttattt ctaataaata taatgtgagt
     8401 tggttgtgga atccagcaat aggtgctaca ataacattga tatttggata tatttttagt
     8461 gtcattttaa agaaaaatca taatgcagat gatattaaaa aatacactgc atttggtatg
     8521 agaaatcacc ttatttctca aggaatggat aaagtagatg gagtatctat agtgccattt
     8581 tctttaggta aacaagaact aattgtatta gcttttttta ttatccaata tattatatta
     8641 tttttgatac aattttaaga agaaatacta gatatttaag gagaaatact aaatgaatca
     8701 aaagaaagtt cattataata aatcggctac ctcaaaagat gttgcaagat tggcaggagt
     8761 atctcaatca tctgtttcaa gagcatttgg tagtgcaaat ggaaagggcg taaaacctga
     8821 agtaagagaa agaatttttc gtgtagcaaa cgaaatagga tatgttccta atttggtagc
     8881 acggggaatg attagtggaa aaactaatgt tataggattg atagttggtg atagtctagg
     8941 tccattttac aatagaatta tcaatttatt tgtagaaaaa attcaagaga taggtaaaca
     9001 gtgtctcgtt tttaaagttc ctagacaaca aagtatagat agtataattt caaaagtaat
     9061 tcagtttcaa gtagaagctg tgattattac agcttcggcc atgaataagg tgatggctga
     9121 gacttgtgaa gaaaacaata ttcctgtgat tctttttaat cgttttatac ctggtataaa
     9181 aataagtaca gtctatgtag acccaattga aggtggggga atggctgctg aatatctgct
     9241 taagaaaggt cataagaata taggatatat tcaatttacg agagaaacaa gtgaagaaat
     9301 ggaaaagaaa attggatttt attctaagtt aagacaaagt gggattcata accttaaaga
     9361 agaatctgca aactatgatt atgattctgg atatgaagca ggtaagagaa tgttagcatt
     9421 atcaaatcct ccaacagcaa ttttttgcac aagtgacttg atagctattg gtgtaatgga
     9481 tgctgcaagg tttgagtaca agttgagggt gccagaggat ttatcagtga taggatatga
     9541 tgatattcaa atggcttcat ggaagagtta taatttaacg acgattagac agccactaga
     9601 tttgttgatt gaaaaaacaa ttaacatact taagtcctta ttaaatgaag aaaatccaga
     9661 gtcagttgtt gaaatgttaa aacctgaatt gattgaaaga ggttctacac tttaagtggt
     9721 agatatttaa ttaagtggta gatacctaat taagttgtag atatctgata agaaattttt
     9781 aagttttcaa tcgttctaaa ttgtgagtat aaaaatctac taaatttagt tatgtaaaat
     9841 ataaattata agtgtagaaa ctaggctact agcttttata ccacttcaaa cattactgga
     9901 ggtagttaag tttacatcag tttagtttta tgtagatata atattctgta aatagacaaa
     9961 attaaaatat gtaagtgtct caaaatgaaa tttttagttt atgaggcact tttttgtata
    10021 aaatcaaatg tatttttata actttaacaa taaaaagagg cttatctcta ttttgagaca
    10081 gcctcaaatt tttaaaacta tattagttta cagcttcttt tcctatcaaa tcttttaact
    10141 cttcatgagt catagtttct ttttcaaata agaatttaga aacagaatgt aaatcttcaa
    10201 gattatcctt taacatctct aaagtctctt tataacaaat ttcaactata cgatttgctt
    10261 ccttttgaac cttatcacat aagaaagtct tatcatttcc atcaatagtc ataaatccta
    10321 agttactcat accatattca caaaccattt ggttagctat ttcagtgact ttctttaagt
    10381 cgtcctttgc accagtagat ttatgattaa atattaattc ttctgaagcc tttccagcaa
    10441 gtaatatttt tatttttcca atcaactcat ccttagtata aatatatcta tcttcatcag
    10501 gtaattgaag aacataacct aatgcttgtc ctctaggaac tatagatatt ttttgtatag
    10561 gacaaattcc tactatatca cttacaagag catgtccagc ttcatgatat gctactattt
    10621 ttttctcttt ttcaaccaat gcagagtttt tagattctag ccctgcaata actctttcaa
    10681 gtgccttgtc aaaatgttct gatgtaatac actcactgtc atctctaact gcaaatatag
    10741 ctgcttcatt tgctatattt gaaagatgag ccccattgaa accatgagtt tttttagcta
    10801 aaagttctag atttacagat ttgtctatag gtttgtcatc tgtatgtacc tttagaattt
    10861 caaatctagt gtgatagttt ggtgcaccta tatgaatgtg tctgtcaaat cttcctggtc
    10921 taagtaaagc ttcatctaat aagtcaagtc tattggtagc acctattatt aatacattgg
    10981 aatctttatt aaatccatcc atttcaacta aaagttggtt tagtgtttgg tctttttcgt
    11041 tgttactttc aagatgtctc tttgctccta cagcatctat ttcgtctata aatattatac
    11101 ttggagcttc ttttctagct ttttcaaata gagttctaac tctttttgct cccacaccta
    11161 catatttttc aacgaactct gaaccagtaa cattgaaaaa tgatgagttt gtttcaccag
    11221 ctacagcaga tgcaagtagg gttttacctg ttcctggagg accataaaat aatactcctt
    11281 taggtatttt tgcacccatt ttttgatatt tttgtggtga cttcataaaa tcgactattt
    11341 caaatagttc ttcttttact tcatctaatc cagcaacatc tctaaatgta gtctttggtt
    11401 ttgtagaaag tgaatcatct gtttgattat ctctttccat ttttgtctgt gcaaagacag
    11461 gagctggatt gttgattttc ttaaaaaagt tgtttagcat tttcatattc acacctcagg
    11521 taattaatta ttgttaatca attttatatt ttccatattt actatttttt attatttatt
    11581 tttcatggaa aaaatataca tgttatctaa ataaaaataa atttgtaaga tgtatgaaaa
    11641 aatgaattta tgtatataga gatttctact tatgtattga ataaagtatt a
//
LOCUS       DAMJWU010000107        11656 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMJWU010000107 DAMJWU010000000
VERSION     DAMJWU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.111"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            106..669
                     /locus_tag="PC460_003079"
     CDS             106..669
                     /locus_tag="PC460_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601945.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            1066..2766
                     /locus_tag="PC460_003080"
     CDS             1066..2766
                     /locus_tag="PC460_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601946.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            2936..3553
                     /locus_tag="PC460_003081"
     CDS             2936..3553
                     /locus_tag="PC460_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601947.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            3914..5107
                     /locus_tag="PC460_003082"
     CDS             3914..5107
                     /locus_tag="PC460_003082"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2601948.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            5125..6417
                     /gene="lysA"
                     /locus_tag="PC460_003083"
     CDS             5125..6417
                     /gene="lysA"
                     /locus_tag="PC460_003083"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF2601949.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            6634..7293
                     /locus_tag="PC460_003084"
     CDS             6634..7293
                     /locus_tag="PC460_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601950.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNSNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(7448..8533)
                     /locus_tag="PC460_003085"
     CDS             complement(7448..8533)
                     /locus_tag="PC460_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2601951.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            8724..9440
                     /locus_tag="PC460_003086"
     CDS             8724..9440
                     /locus_tag="PC460_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2601952.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            9498..10076
                     /locus_tag="PC460_003087"
     CDS             9498..10076
                     /locus_tag="PC460_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2601953.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            10470..11342
                     /locus_tag="PC460_003088"
     CDS             10470..11342
                     /locus_tag="PC460_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2601954.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4806 a         1207 c         1992 g         3651 t
ORIGIN      
        1 ccttaaaatc aaaatttgta aaaccacatt tattatgtta gaatatctta tgatattaaa
       61 ttgtaagtgt ataatattat ttagttacta gattgggggg aaatgatgaa taaagtctat
      121 attgtaacaa catatacagg aactatatta tcttacttaa taaggaatat aagtaaaaag
      181 ctgtatacac atgtgtcaat atcattaaat gaaaatctaa aaccaatgta ttcttttgga
      241 aggctaaatc caaggaatcc atttattgga ggatttgttg aagaaaatat aaatcaagga
      301 ttatatgcta taagaaaaaa cactgtgtgt agggtatatt cgttagaagt agataattta
      361 caatatgaaa atctatataa aaatataaaa ttaattagtg attatagaga agattactat
      421 tacgatacaa tggcattaat atatttacta atgaatattc agagacaatc tgaatataaa
      481 tatgtatgtt caaattttgt tgctgacatg ttagaaaaaa gtgagataga cattttaaat
      541 aaacaagcat ttgaagtaac gccaaatgat ttttataatt tatctggctt aaccttagag
      601 tatgaaggtt tattatcaga atataattct agacaacatt cttatgtatc aaaaatcgca
      661 aacatttaaa aacttgattt agagtttttc atataaaaaa atattgctag ataattaggc
      721 tgaagatagt agtttaactt cgataggatt aagaatttgt aaatcttagt tagaatactt
      781 tcttcagttt ttaaatttct gctaatattt gtatttttat aatatttttg gatatatata
      841 atacagaata gtgcaaaaat cccactcctt taggtgcggg atggatagta caaacagtgt
      901 ataaaaagta tttatacata cacgtataat gttaaatata gttgaataag cagaactata
      961 aatatataaa gatatcttaa tatataataa atatgataaa tatttatttt ttgtaaatat
     1021 atggatataa tagtaataat aacatttttt agagaggagt attaaatgaa acgatatctt
     1081 aaaacatatg gagttgcatt aataattgta ataatttatg catttattaa attacctgta
     1141 ctaagattag attttacatc tttattttca gtaggaataa ttttctttgt tgtagcagga
     1201 atactagata tgatgttaga tagaaatgaa agagcatcta aaatggctaa atataatttt
     1261 tcaatagcaa tagctttgct tgtgtatatt gtagtagttc cctttataac ttctacacct
     1321 gtacttcatg caaaatcata tagagaacta ttgggaaaag taactgaaag taaatttact
     1381 gatgacatta gccctgtcag tgtaaatgac attcgtcttg tagatgaaga tatggcaatg
     1441 aaattaggtg acaaaaagct tggagaagtt cctgcaatag ggagtgtatc caaattagga
     1501 aaatttcata tacaaaatgt agatggagag ttgtactggg tagcgcctct tgttcacaga
     1561 gacataatta aatggataac atctctaagt ggtacaagtg gttatgtaaa agtatcagct
     1621 agtaatcctc aagacgtcca attagttcaa gaaatagatg gaaagccaat aaagatagtt
     1681 taccaaccag aagcctactt acatcaggat ttacaaagac atttatacat acatggtatt
     1741 gtaaatgttg gtatgacaga tttcactctt gaaataaatg atgaaggaag accttattgg
     1801 gttgtaagtt tatatgaaca taaaattggc tatggaggag caaatgcaac aggaatagcc
     1861 actgttgatg cagaaactgg aaaaataaat gtttatgatg ttaaaaacac gcctaaatgg
     1921 gtggatagaa tacaaccaca aagctttgta acagaccaaa ttaaagactg gggtgtttat
     1981 gttaatggat ttttaaactc tgttatatca gaaaagggag ttttagtacc aacagaaggt
     2041 acatcactag tttatggcaa tgataacagg tcttattggt atacaggtat aacatcttca
     2101 ggtggagatg aatctacaat tggatttatg ttagttgatt ctagaacaaa agaagctaga
     2161 ttatataaac aaccaggagc tactgaaacg gctgcaatga aatcagctga aggtaatgtt
     2221 caagaaaaaa attatgaagc gacgttccct gtaatgtata atatattggg tcagccaaca
     2281 tatgtatcat cattaaaaga taaggctggt cttgttaaga gagttgcatt tgtatctgtt
     2341 gaagattata atgtacttgg aataggtgaa gataagaatg aagcattaag aaactataag
     2401 gatgcattag aatctaaggg taatgattta aaacttgata atgatttaaa agatgaggta
     2461 ttagaaggca cagtgacaag aataagccca gatgtgcgag gtggtaacac taattattat
     2521 gtaactttag attcaaataa agatataata tttagggcta cttcaaaagt atctagcgaa
     2581 ttgccattga ctcaggttgg tgataaagtc aaaatatcgt attcaaaaga agaatctggt
     2641 gtaatagaaa tgtcagaatt tgataaccta aatattgcaa actttgaaga taaaaaagca
     2701 aaagaaaaaa caggaactga tgaaaataca gattccaaag attctgaaaa tcaaggtaat
     2761 gtataattta aataaaaaga ttgataatag tatttaacat agtgatagct aatttttata
     2821 gaatttaatt ctataaaaat tagctatttt attttaattc tttgtaaatt taatgtcata
     2881 aaattaatat aatattagta caactttgca aataatactt caaggagaat gtactatgac
     2941 gaatatggaa aaagagatat tacttttaat tagcagggtt agatgtgtta cagagtctca
     3001 atttagaaaa atttatggag accaaaaaag atacaataaa aaaaatttta aaaaaacttt
     3061 gagaaaaatg tgtagtgaat atacactaaa aaaatatcct tgtgatgtgg attattataa
     3121 ttataaagac ctctcttatg tatactattt aaacggtagt aaacttttta agggagatga
     3181 tttaataaag gctctagtag gatcagaatt agttgttaga atcaatactg caggttgtga
     3241 tgtaaaaaga ttttaccgaa atgttagtgt agatgatgtg aaatacgatt tatttattga
     3301 atatacagac caatttggta atataaagca agttttagtt gatgttgact taaataatga
     3361 aatagacatt tctaaatacg aaaatctttc taaaaaaata gataaatcta caattccatt
     3421 ttttaaagta ccaaacgttt tggtagttac accagataat ctatccgaat taaatgattt
     3481 agagagatat gataatgagt atagcataaa ttttatagat ttaggattaa ataaactatt
     3541 taattgtata taagctaact ttagaaaaga aaactatcga tttgccacat tttgtaaaat
     3601 aattgttgca atattatata gaatatgata aaactatatt aataaattaa ttaaaaataa
     3661 tgagcctagg gtagaggtag ctgtattcaa tagtattcaa taggagctgg taagcgttga
     3721 taattgatga aagggtatat agccgaagaa attttgttga cggacaaatt ttctggactt
     3781 atgcagaata tgcataaggc tgtcactttt aaagtggtga gctacaaggt tacttggtct
     3841 atgaaattta cacgtccaaa gtataccttt gggcgttttt atttatcaaa atttagcttg
     3901 agggggagaa agcatgagta taattgttca aaaatatggt ggaagttcag tagcagatac
     3961 tgaaaaaata aaatcaatag cagaaaatat aatagaacga agaaaagaaa atccacaaat
     4021 ggtaatagtt gtgtctgcca tgggaaaatc cactgatgaa tatataactt tagcaaagga
     4081 attgagtaat gaacctagta agagggaatt agatgcttta atgtctactg gtgaaatgat
     4141 ttcagcatct ttattatcaa tagcattaaa tgcacttgga tgtaaagcta taagttataa
     4201 tgcttaccag ttaaatataa aaacgagtgg tcttcatgga aagtctcaaa ttgatgatat
     4261 aaacgtaaac agaattaaaa atagtttaga tgaaggaaat gtcgtgatag taactggatt
     4321 tcagggaatc aacgaagatg gtgatgtaac cactcttgga agaggtggtt cagatacatc
     4381 tgctgtagct cttgcggtta aattaaatgg aaaatgtgag atatatacag atgttgatgg
     4441 aatttatttt acagacccaa gaaaatattc aaaggctagt aaacttgatg agattgaata
     4501 tgaagaaatg cttgagttag cgagtttagg agcacaagta atgcattcaa gaagtataga
     4561 gcttgcacaa aaatatggtg ttgaaatcta tgtcggacgt acatgtggta cagaaaaagg
     4621 aacatatata agagggggaa aagacatgaa attagaggat aaagtaataa caggattagc
     4681 tactagtgat gatgacagtt ctataacaat aaaagatttt aaagctgaaa atatatctag
     4741 cttatttgaa gatatagcaa ctataggtat aagtgtagat atgataagcc aaactgcacc
     4801 tattttagat aagataagtg tatcatttac agttccaaaa gaagagttgg gagaatgtaa
     4861 aaagatagta tctaaatata cagatgaaga acatgtagtg attgataata atatcactaa
     4921 attttcttta gtaggtcttg gtatgaaaaa tacttctgga gttgctgcta aagtatttaa
     4981 gatattcaat gaaaatggta taatgataaa acttataact acatcagaga ttcgtataac
     5041 ttgtgctata aattctgatg ataagcaagt agctatagaa aaaatagcag aagtatttaa
     5101 tatataaaat ttggggaggc tattatgaaa cttcatggaa ctatgactgt aaataatgat
     5161 gaattatata ttggaggagt gagttgttta gaacttagta aaaaatatca aactccactt
     5221 tatgtatttg atgaagcttt agttagacaa aattgtagag aatatgtaga atattttaag
     5281 gctagagagg gaaaaaataa ggttgcttat gcagggaaag cttttttacc tctttacatg
     5341 tgtaatctta taaatgaaga acaaatgtgt ttagatgtag tttcaggagg cgagttatat
     5401 acagcttata aatcaaattt tcctatggaa aaagttttct ttcatggaaa caataagact
     5461 aaagaagaaa taaacatggg attagattta ggtgtaggta aatttgtggt ggataatttt
     5521 tatgaattag atttgcttga agcattatgt gaagataaag gtaaaactca agagatatac
     5581 tttagaataa ctcctggtat agaagctcat acacatgatt atataaaaac tggacagata
     5641 gattctaaat ttggatttgc tttagtaaat ggagatttat ttagagcaat agaaaaatta
     5701 agtgaatata aaaatattaa acttgtgggt cttcacgctc atataggttc acaaatattt
     5761 gatgtagaac catacataga tacagtggat ataatgatga acttggtaaa agaaataaaa
     5821 gaaaaattca acatacaact aacagaaatt gacctaggtg gaggagttgg cgtttattat
     5881 agcgaaggag ataaacctaa aactataaaa gagttttgtg aaaccataat cactaaagca
     5941 gaaacttcat gtaaagagct tggaatagaa gtacctacat tggtaataga accaggaagg
     6001 tcaatagttg caaatgcagg aagtacattg tatacagtag gttctataaa agatataaaa
     6061 gatgttaggg tttatgtaag tgtagatgga ggtatgacag ataatgtaag accttcgttg
     6121 taccaagcta gttatgagtg tagtattgtg aataaaatta atcatgaggc tatgaatcat
     6181 gtaacaatag ctggaaaatg ttgtgagagt ggagatatat taattggaga tataaacata
     6241 atggatatta aaagtggaga tatacttata acaacatcaa ctggtgcata tggatattca
     6301 atgtcttcaa attataataa aataccaaga gcagcagtag tatcagtatt tgatggaaat
     6361 gataagataa tttgtaagag acaaagctat gaggacttat tagctttaga gatataaaat
     6421 ggagaagtat ataataaaaa ttaaatatta aagtttatta tgagcagatt tgctaggata
     6481 tgtatctcta aattaatttt tggtttattg tatcgttcgt agcatatctg cttatttttt
     6541 gttttactaa caaatttctt gtaataaata taaaatattt taaaaacttt ataattatga
     6601 taaaatttaa agtatatgac aaggagggat ttaatgagag gaagagtaat tttattaata
     6661 tcgatattat ctatatttct tgtaggatgt tcatttaata aaaaagatga aataaattta
     6721 gtagagcaag gaaaaacata tttagaaaaa cataactata aaaaagctat ggaatcatta
     6781 tcctctgcac tggaagaaga tagtactaat gagaatgcaa gagctatgta tatgcaagct
     6841 atgagaatgt caaatatgac ggagtttgaa gaattaaaaa attatgaggg tgcaatcaag
     6901 gaacttaaac ttatagagaa aatcaaaaat ggttcctctg ttattaaaga agaggcaact
     6961 aaaaagaaag aagagttaac taaattagaa aaagaacaaa acgaagcaga ggaacaaaga
     7021 aagaaaaagg caaaagatac ggctggagaa gataggtata gagttgaatc agatgcaagg
     7081 aaatcttctt atagtggaag tagtaaaaat aataaatcta gttctaaaaa taatgataaa
     7141 aaatcagata gtaataagtc tgacaatagt aacagttctt ctcaaaatca agaaaagcca
     7201 aataatagta atccaacacc agcaccaact ccacaagata ctactggtgg ttcagatagt
     7261 ggtggaaatt ctcaaaactc aaataatcaa taagtataaa atatatgcat aaatttaatg
     7321 aacaaattat atattataaa aaatatctct tagcaatttt ttcttactaa gagatatttt
     7381 ttgagaaata atttaataaa taacagttaa attttaaact aaaaattaaa gtcacagtag
     7441 agataagcta ttgtaaacga ttatctatat atttatcaac ataagttttt acaacagcag
     7501 ctattggtac tgaaagaaaa actcctatga aaccaaatag agattgtccc accaaaatag
     7561 cagatattac ccatatagct cctataccaa cttgattacc cataactttt ggttctataa
     7621 aatttccatc taattgttgc aatataaata gaaatgcaat gacccatata gttttttgtg
     7681 gactatataa tatagtcatg gctattggag ggattgcacc aataaaagga ccaaaatagg
     7741 gaattatatt acctaagaat attattgaag ctaaaaataa agcattttct attctgataa
     7801 caaattgaaa tcctaaaaat gcaattatac caataataag agagtctaat attctaccaa
     7861 gtaaataatc atagaaaata tcatgagtag tcctaaaaaa ttctataatg ttatctgcct
     7921 tgtttctagc agtagaagaa tatagaaatc tcttaaatcc caatgcaatt ttttctttgt
     7981 catatataat gtatatagat agtataaatc ccattgtcat attaaatagc atagaagata
     8041 tactaagtgt atagattaag aagtttttag gaattttacc aacaaaatta cttgcttctt
     8101 ttaataaatt atctaaacta tgttgtatat gaggaattaa agtttttaga aaatatatgt
     8161 ttgacatatt tttttctata aaactatcta tataatcagt atacataggc atttcattta
     8221 tcaatctatt taatgtatta gctattgatg gaattaataa cctaatggca aaggctaaaa
     8281 taaaacctat aaatatataa gataaaaaaa tggctaaaag tctatgtgct ttgaatttta
     8341 tctcaaaaag catcacaagt ggatttaaaa gtaaagcaag taaaatagct aataaaaatg
     8401 ggctaaaaaa ctttaagaat ccttcaatac tagatataaa gtctgatgga gtattaataa
     8461 atttaaaaaa taaaattcca ataaacacga ctattaaata atattttata ttttcctttg
     8521 aaataataat cacttcctta aaatagtgtt tagtcagcta ttacatatta atagtatgtg
     8581 aaaagaaatt acaattattt aggaaaatta aataattttt ttgcttgttt ttaattaagg
     8641 tctagtatag tataataatg tagcaaaatt aacattgata aaaatattaa acacacataa
     8701 tttaagataa ggaaggatta agtatgagaa ttaataagta tatagcttct tgtggaatag
     8761 catcaagaag aaaagcagaa gaaataattc ttggaggaag aataaaggta aatggaagtg
     8821 tagtaaaaga gttatctttt aatattgatg aagaaaaaga catagtagaa tttgataata
     8881 aaaaagtaaa accaagtgaa aattatatat atatagtatt aaataaacca gagggatata
     8941 taactacagt aaaagaccaa ttcaatagac ccagtgtaat agacatactt aaagatgtaa
     9001 gggaaagagt atatccaata gggagattag attatgaaac tagtggttta ttaatactta
     9061 ctaatgatgg cgatttaaca tataagctta cccatccaaa gcatgaaatt gataaaatgt
     9121 atgtggcaag tgtgaaggga attataagtg atgatgagat aagacaattc gaaacaggac
     9181 taaaaataga agattacaca acggcacctg ccaaaataag ggtaactaaa gaaaataaag
     9241 aaaaaaatta ttcagtgtgt gaaataacta tacatgaggg tagaaataga caagtaagaa
     9301 aaatgtgcaa agctataaat catccagttt taaatttaag aaggatttct gtggggaaaa
     9361 tagtattaaa agacaccaaa gttggagaat atagatacct aacagaagat gaaataaaat
     9421 atttaaaatc aataaaataa gataaaaata aattgagaaa aaatgaaata aagttaatag
     9481 tagggagaaa tagtattatg gcatatgcaa agtatttaac taaaattaca gatataaata
     9541 aattttattt agaaaatatt ataaatgaag gtgacatagt aattgatgct actatgggaa
     9601 atgggtatga tacaaggtat ctagctgaga aggttggaga aaagggcttt gtatatgctt
     9661 ttgatataca agaagaagca ataaagtcta caagaaagaa actagagaaa gaaggatata
     9721 cagatagagt aaaattgata tttgatgggc atgaaaaaat gaatcaatat ataaaagaag
     9781 aagtttcatg tgttttattt aatttgggtt atcttccaag agcgaaacat tcaattataa
     9841 ccaaaccaga tacaacttta aaggctataa agttaagttt agaaatgtta aaagaaaatg
     9901 gagtaataag cattgccata tatacagggc atgatggagg tcaagaagaa aaagattgca
     9961 tatataacct tgtcaataat ttaaatcaag atgaatttaa tgttttagaa tctaagtttt
    10021 taaatcaagt caataaccca ccacaattga ttttaattga aaagaaaaaa gcataataaa
    10081 tactaaaaaa cttgacaaga ttgtattaag agttaatata tatagtgtga cctattaaaa
    10141 atactttaaa taataagtat acaacagttt attctcaaaa caaaatatac atctaaatat
    10201 gcaaaataag atagataact tttaaatgag taaaaattga aaaatttaaa agaaatattg
    10261 gataaatatt aaaaaatgca aggaaagatg gtataataaa tttaatcgaa aatactatgt
    10321 gaagtataag gtacagaaaa gttagaattt tatgagttta agaagctatg ataaaaacta
    10381 agatgaaatt gagataaaaa ataaaatttt tagagaaaaa gacagtgtta ataaaacaaa
    10441 atagtttata taactaaaag gagaaaaaaa tggaagaaac aaatgaaatg aaggacagta
    10501 aacatttgat atcaaatata caatctcaat atacgagatt gagtaagggt caaaaactta
    10561 tagcacaata cattttaaat aattatgata aagttgcatt tatgactgca tgtaaattgg
    10621 gtgaaactgt aggagtaagt gagtctacag ttgtaagatt tgcaaatgca ttaggttata
    10681 gtggatatcc taaattacaa gctgcacttc aagaactcat aaaaaataaa ctaactacag
    10741 tacaaagagt tgaaatggca catgattatt ccgatgattt tgcaatacta aacaaagttt
    10801 taaaaagtga tatagataat ataaggtcta cactagaaga aattgacgag agagcgttta
    10861 aagaagcttc taacaaactt ttaagagcga gaaaaatata tatacttgga atgaggagtt
    10921 cttttgtagt agctcaatat ttaggttttt acttagatat aatacttgac aatgttcata
    10981 taattagaat ggatatggga gatgcttttg agcagattgt aagaataaat gaagaagatg
    11041 taatagttgc tattagtttc cctcgttatt ctaagaaatc ttatcaaatt gtcaattatg
    11101 ctaaagaaaa aggagcccat gtaatatcat taacagatag cttatttgcg ccagtagcat
    11161 cacttgcaga taacactctt ctagttaaga gtaatatggc ttcatttgta gattctctag
    11221 ttccagctct tagtatttca aatgctcttg ctatatctgt tgggatgaag gaaaaagaag
    11281 atattaaaca gcattttgat gatttagagc aaatttggaa aagatattct gtgtatgagt
    11341 aaagtttgtg tattaataag gctatgaaat tgagaatcat agccttatta ataaatacta
    11401 gattatcact taaaaaataa attttcgttt aaataataca aataaatatt tcgagactca
    11461 aaacataaag atgtattctg aatatatctt tactacatca actaatttat taaagaaagc
    11521 atttcccttc atggagagtg ctttttttat ttctctaaac aatatcattt ccttaattaa
    11581 caattataaa attaatttta tttgtattta agtatataat atcttattaa aattctatta
    11641 aaatatgtat ttctat
//
LOCUS       DAMJWU010000108        11633 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMJWU010000108 DAMJWU010000000
VERSION     DAMJWU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.112"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(353..1909)
                     /locus_tag="PC460_003089"
                     /pseudo
     CDS             complement(353..1909)
                     /locus_tag="PC460_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
     gene            complement(2694..2882)
                     /locus_tag="PC460_003090"
     CDS             complement(2694..2882)
                     /locus_tag="PC460_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601955.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            complement(2908..4047)
                     /locus_tag="PC460_003091"
     CDS             complement(2908..4047)
                     /locus_tag="PC460_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2601956.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            complement(4584..5423)
                     /locus_tag="PC460_003092"
     CDS             complement(4584..5423)
                     /locus_tag="PC460_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2601957.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(5447..6205)
                     /locus_tag="PC460_003093"
     CDS             complement(5447..6205)
                     /locus_tag="PC460_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2601958.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(6252..6725)
                     /locus_tag="PC460_003094"
     CDS             complement(6252..6725)
                     /locus_tag="PC460_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2601959.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSYNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(6754..7170)
                     /locus_tag="PC460_003095"
     CDS             complement(6754..7170)
                     /locus_tag="PC460_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2601960.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(7217..8269)
                     /locus_tag="PC460_003096"
     CDS             complement(7217..8269)
                     /locus_tag="PC460_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2601961.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            8665..9345
                     /locus_tag="PC460_003097"
     CDS             8665..9345
                     /locus_tag="PC460_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2601962.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(9478..9798)
                     /gene="sugE"
                     /locus_tag="PC460_003098"
     CDS             complement(9478..9798)
                     /gene="sugE"
                     /locus_tag="PC460_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF2601963.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            10180..11007
                     /locus_tag="PC460_003099"
     CDS             10180..11007
                     /locus_tag="PC460_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2601964.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGSKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         3777 a         2016 c         1294 g         4546 t
ORIGIN      
        1 ttgctgcttt taatgttact tttgatgctt ctaaattatc tgctgttact tttcctacta
       61 gttttgttac ttctgctatt ttttcttctg cttttgtctt tgctgcttct ttagcctgtg
      121 attctaattc agttatctca ctttgtactt ttgctatatc tccatatcct ttaaagcttt
      181 gaacttctga ttctgtccac cctgctttcc ttgcttcttc tattgcatca gttgctgctt
      241 ttaatgttac ttttgatgct tctaaattat ctgctgttac ttttcctact agttttgtta
      301 cctctgctat tttttcttct gcttttgtct ttgtttttga cttatcgctt ggttttgatg
      361 aagaacttcc tcctccatgt gaagagcctc cacttgatga acttcctcct gaagagttag
      421 aacttggact atttccactt attgttggag aattatctcc cttaacacta cttattgtac
      481 ctttattatc tatagtagtc cctttagaat tatttgtaac tgtagttatc ttaccaccat
      541 tagtcacttt tacttgactt gctccagatg ctactgttaa actaccaatc tcacctttag
      601 aattattttc tatcttggct ccatttttat cattaacctt taagttagtt atttttccat
      661 agttaacaaa agttccatct tttacatcat ctataactac atcatccact tttgcatagt
      721 tatttacatc tccattaggc atgttgatag ttacagtctt tgtatatgta ccatttaagt
      781 ttactgttac tgctttatca gtttgtattg taaatgcttc tttaacctca ctagttggct
      841 taaagtttat aacatcatta gcagtagctt tatctatagc tgattttaat tcagaaattg
      901 ttttagcttc aactgttttt acttcttgta atgacttcat ttcattaaat gctgtctcat
      961 taccattccc accaacttta gttatcttat caaaactctt agagttaact agagattttt
     1021 gtgaagctga taagttcttt ccaactaaaa ctactggtga ttgattttta gcacctaata
     1081 cacctactgc tagtgcatct ataagttggt cttgtttatt cataccatct ttagttacat
     1141 aagcattttt taaatctgtc tttttataaa attcttttat tacttttgca tttgtatcat
     1201 ttctatctgt tccacctagt cttgtactat ttggtagttt atttttaaca gcttcagaaa
     1261 ttgaactctc tccacctact acataagatt gagttattcc agcatctttt ataaatttgt
     1321 ctgcaactgc tgttccattt ttagaatcag caagtattac tggcatattg ttttgtgcag
     1381 ctggtgctcc tacacttaca gcatctgcaa gtcctttttc accattaact acagctattt
     1441 tcttaacatc acttatagca tctaatttct ttgctaattc taaggatgtt tgatatctct
     1501 cagctccaga gattctctca aaagatattt ttaaatcttt tatttctttt tctatatttg
     1561 tacttaatac agaagttcca cctattaagt atactttttt agcttttaat ctttgtattt
     1621 ctgctttcgt cttatcattt aatttatctt tacttgttaa aagtattgga gcattttttg
     1681 cttttgcaaa tggtgttgca gatagagcat ctgctataga tgagtcgttt acgattacta
     1741 cctcatcagc atttttccat ccatcttgac ttattttaac agcagtttca tatctttctt
     1801 gccctgttaa atttgttgcc acagtttgtg cactagctat gcctgctgtt gaacttccag
     1861 ctatcattgt aactgtaagc attgcagcaa tcttctttgg tattttcatt ttattttctt
     1921 cccccttgtc catttgatat tacttaattt tttaactttg ttcccgttct aattctaaag
     1981 gatacttaat atctaatttt aaagaggttt ctaactataa tgatttcata attaatttgt
     2041 aattattttg ttacattgca gtcattatgt aaggaataaa gaaattttct tattgattga
     2101 aaaatttgta aatttatatt ttaaggtaag tttgattttt atgttaatga attgttataa
     2161 aaaatagggt tttgtataaa aaaagtagcc tttatgaatt aaaagtgcta ctctttttac
     2221 aaataaattt tattatttat acttgtaatt atatattaaa taaccaattt tatccatatg
     2281 aaaaacaaat aattgcgata tttttttaat tattctttat atttacattt taattcaaat
     2341 tacttttgta aagattatat ctgttgcata tactacttta agtgctaata tctctccccc
     2401 tcaattagaa tcctcgttat ctcaatagac gttttctact gtcaaagtta ccaacatttg
     2461 aaacttatta aatttttata taaaaaagac ctagaatata aattccaagt cattttttat
     2521 taaatttatt ttttacaata taatattgtc atgattaaag aacacaaatc atacacaaat
     2581 tggtcataaa tcttacataa atcatctaca aaaaatacat tatttatatt aaatctatat
     2641 aactaaatta agagcatatt gtacaatatg ctcttagaaa agatatttct tgtttatagt
     2701 ttatagtatt tctctatgat ttttattttt atcatttcaa aaataaaaat taagattaca
     2761 gttattatca tattttgtgt actattttct ataatattat aatgaaacaa tgaaaaaaca
     2821 ataattaatg aaattacgtt tatagcccca gaaatcaaaa atgccttttt cttattattc
     2881 atatattccc tctccagatt atctaaatta gtctatatca agcattttta agtacttatc
     2941 attaacttta tatatatttt tttctttaga caaaatctct atctcttcaa gattattaaa
     3001 cacttccatc aactggtcaa ctaattcaat ttcctttatt ccagccttta agtatctact
     3061 tttattcttt tttatataat tttctaatgt catagaatag ttatctttat tgcaaatata
     3121 ttttaatata tgttcttctg ttgctgatag agttatgtca ttttcaaaca attcttcttt
     3181 taatatactt tctaaagatt ttctatcatg catattacgc atctcattaa tagattgtat
     3241 tatagacttg agcataaatt ctataaatac agttaaatta tttcctttgt tttctacatc
     3301 aagtatagcc ttataatact tagttctgtt tttagatatc atatatgata ttgaaaaatc
     3361 cttgaaagta tcatatcctt tatcaatcaa atatagatat gataaggctc ttgatgttct
     3421 tccattacca tcactaaaag gatgaacata tacaaagtaa aaatgaatta tagcactttt
     3481 aattaaatta cttactttgc tattttccat aaaatctatt aatttactca taaagtcata
     3541 tatcttcaaa ggttcaagcc ctgtatgtat aatctcacct tttgaatcac atacatatac
     3601 tttttcagtt ctatactcat attcttctga atccaagcag ttttcgccca atatatgatg
     3661 caaatcatat ataaatttgt gactatataa tttatctaag ttgtctaatc catattctaa
     3721 ggctctatga ttattgtaaa tcatatattc acttttatta cttggtttca tctttcctct
     3781 aattatagat tttgcaatct ttctagttga aaaagcacct tctattacac tagaataata
     3841 agactctagt gccaaatcat ttttatcttc tattgtttta aatatcttac ttgatgttaa
     3901 tttatctatt tcatttatta aatttaatat attttcagtt tcatgatagt atagttcttt
     3961 attatcaaaa ctatttatag aaagtacgat tttatttcca gtaaagtatt tgtttttgta
     4021 ttcttctatt gtcagtttaa tcaccacctt agattatctt tatctattaa tattaacaaa
     4081 aagtattaat ttttacacta tttttgatta tttgttttat aattatcaaa ctcaatttag
     4141 cgttaaagca taataaattt taataaataa tgcaaaaatt taagaataag catttaaaat
     4201 atatgtcaca ggaagtttac aactattatt tataatacat tagttacata ttataatatc
     4261 tcgacaatgt cttaacaata tctcgattat atattttaat atataactaa tatgttataa
     4321 tattttaatt aaattaaata aatttatatt tttttgaaat cacttaatat tttatataaa
     4381 aatgaaaaag cacctcattc tgattaaaaa tctttctgag atacttttta aaatattaaa
     4441 tactacatat agcttacata agtttaaaat ttaatctatt ataaaattac cctaattgcc
     4501 aaacttttta atataaattt tccaacatcc tacaaatata atcaatagat atagaatcct
     4561 aacttacacc aatatatatt tatttaaaat agtcctaaaa tacctgtaat agctcccact
     4621 actatcagta taaacattat ttttattgga gaaagacctt tttttaaagc aaagagtact
     4681 aataaagtaa gtaccaaagg aattaaccca ggaaatatct tatcgagtaa atccgtttga
     4741 agactaaatt taaaatcacc actagtataa cttattggtg tagataaatt aataaaccta
     4801 cctacaagtc cccccatgac taaggtacct agtatagaag ccccttctat aaccttattt
     4861 actattccac cttgaagtat actttcaacg gcttttttcc catacttata tccatttgtc
     4921 cacattccaa aagaaattga agtcattatt atatattgag ctattacaaa taatataggt
     4981 cctgcaattg atgctcctcc ctcagttaaa ccaatacaaa cagccaacac tataggagtt
     5041 ataactcctt gagttaaagt atcgccaatt cccgctagtg gacccatcaa gcctgttttg
     5101 atagcattca taccatctcc acttatttca tctgctccat tagctttttc ttcttccata
     5161 gctattgtta ttccatgtat aacacatcca catataggtt ctgtgttaaa aaatgctaga
     5221 tgagtctcca cttcttgttt taactcttcc ttatctggat ataatttttt aagtactggc
     5281 aacattgaat gtgaaaaagc tgttgattgc aatctttcat aattgtaatt agattgtgca
     5341 aagaaaaacc atcttaacca tgattttatt acatctttct tgcttaacgt tttcttagat
     5401 acttcatttt ttatatctga cataatattt tccccctcac atgtatttaa gatgtagcac
     5461 tatcttttcc gtacttaatt gaagtaattg taagagcaag tattactcca ataacagata
     5521 ttactattat atctacttta aaataaacag ccattagtat tcctaatatg aaaaagggca
     5581 tagtaaactt gtttccaatc aattttaaat tcatagcaat cccaagtgct ggcaacatag
     5641 ccccaattac attaaataca tgtaacacat tatcattcat tacttccaac ataccatcta
     5701 tcgcatttgg accaaaataa acaactaata atgctggtat aaaactcatt gcaaataaca
     5761 gtatctgtgg tggtactata tttataaatg ctactctatc aacattccct ttttgaacct
     5821 ctctatcagc ccaatgtgca aaaaaagaat ttactgtcat ctttccaatc caaagtacag
     5881 ttgcaacagt tcccaatggt acagctattg ccattgcagt acttacatct agcttagatg
     5941 ctatagctaa agcagtacct acatatcctg caaaagctgg gtcccccatc tgagctcccc
     6001 ctgctgatat aaaacctaga tatatcatat ttatacctgc tcctataatt gctccttgaa
     6061 cagggtctcc taatataatt cccgttacaa atccagccaa tagaggtcta ttccatgtaa
     6121 aaaatccaac ataagtcaac cacggactcc aagaaagata ataaaaaatt gctattaata
     6181 ctgcttgaat tagtgaaatg tgcatattct tcctccctat tttcttttaa agatttacaa
     6241 gatagtcttc tttacaataa tgttcctaca tcaattttct ttgcatctgg tactatttgt
     6301 ataaagcatt taacatcttt tgatattagc tctctaaata tttctttttc ttcatctgac
     6361 acagatatat ttttataaaa actttttcta ccaagccctg ctcccatacc tccaacattc
     6421 aattccttta aatctacccc gctatttact aaacctaaga cagttttagg gtatttgacc
     6481 aatactatta aattttcatc tccattatat gattttaaga cttcagcacc tgattctaca
     6541 tcatgtactt caaccttaac agttgatgga gctgccattt gtaaaacttg aactataaat
     6601 gggtccttta ctactaaatc atccacgatt actatccttt ttgctgatgt aatcttacac
     6661 caagcagtaa taacttgccc atgtattaat ctatcatcaa ttcttgttaa tgctatatta
     6721 ctcataatta ttcctccaga tttttaattt attttacaac tttaaaacag ttttaacatc
     6781 tttgattcct gatatcccaa cttcctcaca taaattcttc aagtctttta acttcatagt
     6841 atctctgttt aaaaataact ctaaaagcat aggtaaattt acacctgtca aacattctaa
     6901 ctcacatacc ttagcaaatt tatttattat aatagcagat gtattaaatg gagttccgcc
     6961 ataaaaatca agcaatacta aaaccccttc atccttatat ttctcaattc catcttctaa
     7021 ctttctacta aactcatcta tgctgtcacc atggtttaat cctaaagttt ctacattttc
     7081 ctgtttgcct actattaatt cagcactttc taacattgct ttagccatat ttccatgtgt
     7141 ggctataatt attcctgcca tatgaatcat acctttctta acctttttta tatggattat
     7201 tatttagtac tctaatctaa ttatctatat taatcattat cttaccaaag aaaatatccc
     7261 ttttgtatac tttttcaaaa taagctggta cttcttctaa atttattcta tgtgttacta
     7321 atttttcaac attcatgtca cccttttgca tatgatacaa tccagcactc cattcttttc
     7381 ctgggaatgg aaaggagttt ccaaaccaag ttccctttac agttaattca cttcttacta
     7441 tcttttcaaa ttgctctcta gtcaaagcta catccccata tggtacacct gcatacaata
     7501 ctgtacctcc ctttttagct agcaacagta cttgaccaca tgtcagtgga gtaccagccg
     7561 attcaataac aatatctgca ccatctccat cagttaacct ttttatttct tctacaatat
     7621 tcttttcttt tgcatttata caaatatctg cccctaactc ttttgccaag tctaacttct
     7681 catcaaatac atccacagct attatttttg tagaaccaaa tatttttgcc cattgtattg
     7741 aaaataatcc tattggacct gtaccaagta ctacaacagt atctccaact ttagcttctg
     7801 acctaaaaag accatgtcct gctatacata ctggttctag cgcagcggca gtctcataac
     7861 ttatctcatc tggtattttt attaaatttc tctctttcac ctttgtatac tctgcaaaac
     7921 aacctccaag ttctttattt cctattattg ctacattatt acatcttgaa tacaagcctt
     7981 ttttacattc atcacattca aaacatggca tagctggaca aacagcaact ctatctccta
     8041 ttttaaaact ccttacttct ttaccaacct gtgccacttc tccagaaaat tcatgcccta
     8101 aaatttctcc aaccatatgt ggacctgtct ttgaatattt tgatatatca gaaccacata
     8161 ttccagctac ttttacttta attattacat catctttttc taatattttt ggtacatcca
     8221 catcctccac tcttgtgtct cttataccat agaatctaac tgacttcact attaaccacc
     8281 cctacttttt atttgagaat atatccctat tgtttatatg ttaaatatct aaagcaactt
     8341 tcatgccaat atatgtaagc attgtattca tgcattttat aaaattatca ttaaatgtgt
     8401 gtcaaagtgt gtcaaaagtt tcttatatta aaacttatgt gtgtcaatat aaacgacact
     8461 aacattttat attagttctc ttattataag ttttataaaa tgtacttttt ttgttatttt
     8521 tataaaattg ttcggaaatt tcccttctat ttgtgatata atattaaata taaggtatca
     8581 atataaattt tcatgcgaat atcagaactt ttttgacact aatgccttat tcaatataat
     8641 aatttaactt tttggaggtt gtttatgaaa aaagtgtatc aaggaaagac taaagatgtt
     8701 tttgaattag ataatggaaa ttacttactt aaatttaaag acgatgtaac tggtaaagat
     8761 ggagtatttg acccaggcga aaatagtgta ggtctaagta tagatggaat tggtagagct
     8821 aatttagaga catctgttaa gttctttgaa attctaaaca atgctggaat taaaactcat
     8881 tatgtaagtg ctaatttaga agaagcaact atggaagttc taccagcaaa agtttttggt
     8941 aatggattag aagttatttg tcgttataga gctgttggta gtttcttacg tcgttatggt
     9001 tcatgtgttg aagaaggtgc taaactagat tgttacgtag aagcaacttt aaaagatgat
     9061 gaccgttgtg accctcttat aacatctgaa ggtttagaag ctttaaacat tatgactcaa
     9121 gctcaatttg attcaatgaa aagcatgact caaaaaattt ctaacatagt tcgcgatact
     9181 atagctgaaa aaggtcttga gctttatgat attaaatttg aatttggatt ctacaatgat
     9241 gaagtaatct taatagatga aatagcatct ggaaatatgc gtgtttataa agatggtgta
     9301 atagttgacc ctatggattt aactgctctt ttattagata aatagtatat acatataatt
     9361 tacaaatata ataaacttat aaataagata agctatgaag taaagacttt ttaaatgttg
     9421 attattttta aacattttct cgcctttaat ttcatagctt atttaaatta atatactcta
     9481 attagtagat aatagcttta accctacaat tcccaccaca ataaaaccta tacatgcaat
     9541 tcttgatata ctaataggct catttaaaat agccatgcct acaatagctg taccgacggt
     9601 accaattcca gtccatattg catatgaagt tccaagtgga agatgtttta gtgctaatga
     9661 caaaaaataa aaacttacaa tcattccaac aacagtaagt atacttggag ttaatttaga
     9721 aaatccatgt gaatttttta gttgaaaagc ccaaaacact tcaaacaaac ctgcaacaaa
     9781 caaaaataac catttcataa attcatccct cttttcaaat atgtctctta tttttccaat
     9841 aaaataacct gagaaatatt ataaaatatc tcccaggttt ttgtccttcc gtgttcacaa
     9901 caaaaactat gttgtgggtt ttatctcgga ccagaccaat cattaataat tgcggaaccc
     9961 tataaaactc tattaattat tctattttat ctataataat actatcaaca tatataaaaa
    10021 aagtcaatat atgggttcca tatataataa aaataacttt tatatacctt ttaatattct
    10081 tattcacttc tctccatacc catttgggtc aatctaagaa tatctaattt ttatttaata
    10141 tgttataata acataaaaat attgaaagga gtaatttcta tgaatttcta taaaattgca
    10201 gtatgtcaga tgattactac agaaaataaa attgaaaata tcaatcatgc tgtagatatg
    10261 gtaacagaag ctgccatcaa tggatctaaa attgtagttt tgccagaaat gtttaactgt
    10321 ccatatgaaa ataaatattt tcctaaattt gctgaagaat atccaggtga aacaacaaca
    10381 atattgagta aattagctga gaaacatgga atttatctag tttctggtag tattcctgag
    10441 ttagaagatg gaaaaatcta taatacttgc tatgtatttg ataaaaatgg agctctaatt
    10501 ggaaaacatc gtaaaatgca cttatttgac attgaagtta caggaaaagt tagttttaaa
    10561 gaatcagata ctcttacagc tggaaatgat gttactgtaa tagataccga atatggaaaa
    10621 gttggtatag ctatttgtta tgatattcgt ttccctgagt tatcacgttt aatggctctt
    10681 aaaggtgctg aaatcgttat cttgcctgct gcatttaata tgactactgg tcccgcccat
    10741 tgggagttat ctataagaat gcgtgcttta gataatcaga tattctatgt tggtgctgca
    10801 cctgctagaa atatgaatgc ttcctacata gcttttggaa attcaagaat ttcagaccct
    10861 tggggaagaa taattgctca agctgatgaa aaagaatgta ttatttatgc tgacattgat
    10921 agagatttaa ttcctgacat aagacaacag ttaccacttc ttaagcacag aagaactgat
    10981 ttatatgaac ttaatacttt gaaataaaac agaacataac cccataaatc ataaattaag
    11041 tgttaaaaaa atttatgggg ttctcattaa tttgatttaa aattaatttc ataaaaataa
    11101 tactaatatt aatttaatta aataatacta ataattacta atgattatgc taattatcag
    11161 catttactaa tttaatcact attagttatt tcttttatta aatatcttta ctttttttac
    11221 ttattatgtc ttctagcttt acatttggat tatagtattt ctttctattt ttagtcacat
    11281 ctttctattg aattacttgc ttaaaacacc aatgcacaca ttccagacaa tgttcacaat
    11341 tatgtaaaaa tcttgctcta ccttcttcaa tgttgatatt atctctccta gttgatatta
    11401 tctttcctaa aatttctttt aattaaaaaa caacattcgt tgatatatcg agtatatttt
    11461 aagcaactga aaaaaacaat aataattgat atatcaacta attaatgtag attaattcat
    11521 ttttataatt tatatacttt ttaattaatc taccttttta attaaaattc ttcctttcta
    11581 tttatccatt ttaaacttaa attataagat ttcatcagct gtgtttttgc att
//
LOCUS       DAMJWU010000109        11388 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMJWU010000109 DAMJWU010000000
VERSION     DAMJWU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.113"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            41..925
                     /locus_tag="PC460_003100"
     CDS             41..925
                     /locus_tag="PC460_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2601965.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1024..2199
                     /locus_tag="PC460_003101"
     CDS             1024..2199
                     /locus_tag="PC460_003101"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2601966.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2327..3181
                     /locus_tag="PC460_003102"
     CDS             2327..3181
                     /locus_tag="PC460_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601967.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            3225..4460
                     /locus_tag="PC460_003103"
     CDS             3225..4460
                     /locus_tag="PC460_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2601968.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSQSKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLI"
     gene            4561..5502
                     /locus_tag="PC460_003104"
     CDS             4561..5502
                     /locus_tag="PC460_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2601969.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5721..6074)
                     /locus_tag="PC460_003105"
     CDS             complement(5721..6074)
                     /locus_tag="PC460_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2601970.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            6370..7281
                     /locus_tag="PC460_003106"
     CDS             6370..7281
                     /locus_tag="PC460_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2601971.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            7541..7975
                     /locus_tag="PC460_003107"
     CDS             7541..7975
                     /locus_tag="PC460_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601972.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            7998..9533
                     /locus_tag="PC460_003108"
     CDS             7998..9533
                     /locus_tag="PC460_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601973.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDAILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            9617..10984
                     /locus_tag="PC460_003109"
     CDS             9617..10984
                     /locus_tag="PC460_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2601974.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTAMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            11019..11243
                     /locus_tag="PC460_003110"
     CDS             11019..11243
                     /locus_tag="PC460_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2601975.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         4528 a         1058 c         2113 g         3689 t
ORIGIN      
        1 ttaaataata ttaatcaact tattgaatgg aggaataaaa atgaaatttg tgactttttg
       61 tagtagcaat gaagaaaaaa taggtgtatt taattcagaa acaaatagta tatatgaaat
      121 taatagttta ggattaagta aattatatac tgatatgaat gattttattg aaaacgtatc
      181 tacaggagat ttagaaaaaa taaaaaacaa ttcatttgaa aacgcaaagt gttataagtt
      241 agaagaagtt aaattatgtt ccccaattgt aagacctaaa aaagatatta tatgtttagg
      301 tctaaactat aaagaccatg tcaatgaaat cccagatgga gttataaaaa acgtagttat
      361 gccagattat ccaatttatt tttctaagag agcagacaaa ataattggag tggatgataa
      421 gataagctta catggagatt tggtagagaa gttggactat gaatcagaac ttgcagtaat
      481 cataggtaaa gaaggtataa atatatcaaa agaagatgct tatgaatata tatttggata
      541 tacaatagta aatgatataa gtgaaagagc acttcaagat aaacatgttc aatggtttag
      601 aggaaaaagc ttagacacac atacatctat gggaccttgt atagtacata aagaagaatt
      661 tgaacatcct ttaaaattag atataagtag tgtagtaaat ggagaagtaa gacaagattc
      721 taatacagag tactttatat ttgatatacc tactataata aatgatttat caagaggaat
      781 gactctaaag ccaggagata taatttcaac tggaactcca gcaggggttg ctatggggat
      841 gaatcctcaa gtatatttaa agcatggcga tatagttgaa tgtaaagtcg aaggaatagg
      901 agttttaaaa aatatagttg attaatggat aaatatgcca tataatttta ttagtacgaa
      961 taatttaatt aatataggat taatcaaact tatgattaat ttattataga gagggatgag
     1021 tatatggcaa gagaactaga attaaagtat ggctgtaatc caaatcaaaa accatcaaag
     1081 atttatatga agaatggaga acttcctata gaggtattaa atggaaaacc tggatacata
     1141 aattttttag atgcatttaa tagttggcaa cttgtgaagg aattaaaaga agctacaggt
     1201 cttccagcag caacttcatt taaacatgta agtccagcag gagcagcagt aggggtacct
     1261 ttatcagata ctttaaagca aatatatttt gttgatgatt tagagctttc tccattagct
     1321 tgtgcttatg caatggcaag aggagcagat agaatgtctt cttatggcga ttttatagca
     1381 ctttcagatg tatgtgataa ggagactgca actattatag caagagaggt ttctgatggt
     1441 ataatagctc caggatatac agatgaagcc cttgaaatat taaaaggaaa gcgtaaaggc
     1501 aactataata ttgtaaaaat agatgaaaat tatactcctg aacctattga aacaaaggat
     1561 gtttatggaa ttacttttga acaaggtaga aatgaaatat taataaatga agacctatta
     1621 aaagatattc caactgataa taaaattttt acagatagtg caaaacgtga tttaattata
     1681 gctcttatta cattaaaata tacacaatca aattctgttt gttatgctaa agatggacaa
     1741 gtaatagggg taggtgcagg tcaacaatca cgtattcatt gtactagact tgcaggtaac
     1801 aaagctgata cttggtatct tcgtcaacat cctaaagtat taaacttaaa atttaaaaag
     1861 gatataggtc gtccagatag agataacact attgatgtat atctttctga tgattatatg
     1921 gatgtgcttg ctgatggcat atggcaaaac ttttttgaag aaaaaccaga gcctctaact
     1981 ggagaaggaa aaagagcatg gcttaaaact ttaacaggag tagcacttgg ttcagatgca
     2041 tttttcccat ttggagataa tatagaacgt gcaaaaagaa gtggggtaag ttttatagca
     2101 caaccaggtg gttctattcg tgatgataat gttatattaa cttgtaataa gtataacata
     2161 gtcatggcat ttactaagaa tagattattc catcattaaa atactgaaag tataaagata
     2221 atatagatta agtaaaaatc tacaacaagt tttgttgtag atttttttat ttgctgtata
     2281 attttaaata gaactttatt tttagatatt caatttggag gttgatatgg ctttaaatat
     2341 aagaaactat agcctattat gtaatcaagg aacagctaaa tttaatgaag atgtagtcgg
     2401 tataaatcca tttggggcat gggttttaga tggtgctaca ggacttaatg gaaaaaattt
     2461 agtgtcaact gaaagtgacg caaggtggta tgttcagtgg tggaataggt atttatataa
     2521 aaatataaat aaagaagaat cattaaagag tattattaat actggaataa tagaagtaaa
     2581 aaaagagtat gaactaagat taaatgggtt taaggttgaa aaattagatt taccatctag
     2641 ttctatagtt gttgtaaaat ttcatgaaaa taaagtagag tattttttat taggagattg
     2701 tgctttgttt attaaaaatg gagattccaa agttataaaa gataggagta tttgtaaatt
     2761 tgataatact gtatttgatg aaatgagtaa actttctaac ttggaagaga tggcatttga
     2821 tgagattaga actagtgtta tggatacaat agtaagcaat agattgaaga aaaatacaaa
     2881 agagggctat tggatattgg attttgatga ggaagctgtg gaaaattcta tatatggata
     2941 cattgaaata aaaaaagatt ttcaattgat gctaacgagt gatggtttta caagtgcatg
     3001 tgatagatat aatctaataa aagaagaaga attgatacgt atagcagaaa aatttggtat
     3061 aggatatata cataataaag taagggattt tgaagataat gactataaag cggttaaagt
     3121 gccaagattt aaagtaaaag atgattcatc ttgtttatat ttagatatat ataatgatta
     3181 gactgtataa aactatttaa ttttagaaag gaggcaagtg aaaagtgaaa attcttcata
     3241 taataacaca aaaaccaaat agtacaggta gtggtatata tttgtctggt atgataaaag
     3301 gattcgaaaa aatcggtcat aagcaagctg taattgcagg tatagatgta aatgatgatg
     3361 taaattgttt tccaagtgaa gtaagttttt atccagtaaa gtataattgt ggggagttaa
     3421 attttcctgt tgtaggtatg agtgattcta tgccatatga aagtacaaga tataaagatt
     3481 taaatataga catgataaat agattgaaat atcaatttaa agtaaatata gataaggcta
     3541 tgaatgattt taaaccagaa cttataatat gtcatcattt gtacctttta actgcatttg
     3601 ttagggaaat ggtaaaagat ataaaggtta tgtctatttg tcatggaact tgtttaagac
     3661 agttaaatac cattgattta gagaaagaat acataatagc aaatataagg aaattagatt
     3721 taatatttgc tcttcatgaa aatcagaaat atgatattat aaagactttt ggagtaagtc
     3781 aaagtaaagt agttgtaatt ggtagtggtt ataatgatga tatattttat aataaaaatt
     3841 ataaaataaa ggatgataaa ataaaaatag tatttgcagg taagatatgt aaatcaaagg
     3901 gactgattcc atttataaaa gctatatcta aattaaaata tagtaaagat ttaatagaag
     3961 tcaattttgc aggtactgga agcgatatcg aatcatataa tgagatagtt aaactggcaa
     4021 gtaagtctcc atttaaaatg aatttcctag gaaagttaga acagagagat ttagctgagc
     4081 tattcaacag gtcacaaata tttgtgttac catcatttta tgagggatta ccagttgtag
     4141 ttttagaagc tttatcttgt ggcacagatg taatcactac tgatattctt ggtgttaaag
     4201 agtggattgg tagtgagatt aataattctg gaaagataga atatgtttct ctacctttta
     4261 tggagaaaga aggtattcca aaagatgaag agttatatga ttttgaaaat aatttgtata
     4321 atgcaataga ttctaagata caatctttat tgaataactc aaataagaaa acatctgtag
     4381 atatgtcaaa aaaaacgtgg gatggcttag catatagaat aaatgaagtt attttaatgg
     4441 atgaattgtg tttaatctaa aattatttaa attataaaaa agtgtaacta tttttacact
     4501 atttttaaat gattttatat tatactaata ctaaatatat ttgttttagg gaggtaattt
     4561 atgaggatta gtaaaaaggt attggcgtta ggtatttcag cattactgtt aagtgttagt
     4621 tttccaacat caataaacgc actagataag attgaaaata ttcaaggagt agataaatat
     4681 gaaacagctg ggcttatttc agataagcaa gattttacaa cagcagttct tatcaatgca
     4741 gattctacaa tggtagatgg gattgctgca agtggtcttg caggagtaaa taatgcagct
     4801 attttattaa ctaataagga tgatatacca gaagcaacgc ttcaaagact gaatagagtt
     4861 actaagatat atgtaatagg tggagaaaat tctataagta aagatgtaga aaaaatgtta
     4921 cttatgagaa gaatgcaagt tataagaatt gatggtgtag atagagtaga tacaagttat
     4981 aaaattgcag gtgaaataga aaaaattaaa aacagtgata agatgttttt agtaaatgga
     5041 tttaaggatg aagctgatgc agtaagtgtt gcatctgttg cttatagaga tggagctcct
     5101 ataatattga caaaagatat accttcagca gaagaagaca cagacttaga cccatggttt
     5161 ggagtttctc ctgttccagt atatgctatt ggaggagagt ctacattaag tgattacata
     5221 gtgagcagat atagagcaac tagaattggt ggtgttgata gatatcaaac taataaaaat
     5281 gttattgaaa agttctataa tggagcaaaa gagttctata taacaagtgg agacgattta
     5341 gtttatgctt tagttgcatc accacttgca aaaaatgctc ctgttgtttt agtttcaaat
     5401 aaaagtgata agagtatatt aagtggagct agtaaagtaa cagcaatagg tataagtgat
     5461 aagagtatta tagagcaatg tttggatgct gtaaagaagt aatggagtgt tattagtaaa
     5521 atgaatatac acattgaaaa ttagtagatt gttcatttta taatggtata aaatatatgg
     5581 gaagaggcta tcaaatggag ataagcctct ttttcattgt taaaattatg aaaagatact
     5641 ttattttata taaaaaagta tcttatgaac taaatgtcta ttttgatata ctatctcctt
     5701 ttcttatatt atatgatttt ttatatggaa taaatttcat aagtattatc ttcctttaat
     5761 ttttctaacc aaaccatctc taaaatatct agttcttcta aataatcttt ctctgttttg
     5821 ttatcatcat gttttataag ttctaaaact tcaaatgtaa atgctttttc accatatttt
     5881 ttccattcat tatttaattc ttttatcata catgagccag caccaagctg aaatttgaga
     5941 gagttaaatt tgctttttat gtcattactt tttccaaggt aaatcttttt agtaatatca
     6001 ttttttatta tgaatacacc tgtgtcaaat ttcatttctt tgtataattg ttttaattca
     6061 cgttttctat ccatgatttt agatacctct ctttataatt gatatgtaat atgtaagtgt
     6121 aaaaatatat tacatatcaa ttataaagag attaatttta tatgtaaata gtttttataa
     6181 atttaattat gaattagtca tcaataaaca agaaatttag ctctagaaat ccattataaa
     6241 ggctattata aattttaata tgctaaaaat ataaaaagaa tagttgacag tgtagaaaat
     6301 atggaataaa ctaaaagtgt aatagtaata tgaaattata tattacattg aaaggtgtga
     6361 atgattatta tgaataaaaa aatagaatta atagggaatt ttgcaagtga ttggattagg
     6421 tatagtgatt atgaatataa aattactgaa gaagatgaat tatatatagt tccaacagaa
     6481 gatgctgtgt ttaccatgta taacccattt gatgtagcag atgatataat agtagatata
     6541 attcgcatag gacaagaggc attacaagat atatcaaaag agacaaaaga aatgaaacta
     6601 aagaaagaag tacttgaatt tgtaaaaaag tatggattat tgggtttaat atattcaagt
     6661 gtatataata gagatatagt tggagaagaa agagtactga tgattgaaaa gaattatatt
     6721 acaaaagaga atatattgag tacagatgaa tatgtaaaca tgtttatacc atttgcaagt
     6781 gatgaagatg tgtattttaa aaagtataga aggggtatag atgtatcaaa gagagaagaa
     6841 tctccaaagt tttttggaaa gagaccattg attttagatt tagtattttc aaagttctac
     6901 actgaacaac tagattggat tgtaagtttt gctaaagata tgtgtaagca ttttaatcaa
     6961 atttgtgtat ataaaaatac atctaatcta ttaactgaaa gtgttactat tatgtctgga
     7021 gggtttaatc ctcaaaagat aggattttct ataagccaat tagataaacc aattattgca
     7081 tgggagtttg attctttgaa aacagctatt gagactatct atgcctttgc aattacaagt
     7141 gaaccttctt ctttaaataa atgcaagtac tgtggtaaaa tttatattcc tacaaattct
     7201 aaatctcaat actgttcacc ttcttgtaga aattgctcaa atgtaaagaa aagtagaagt
     7261 agagccaaaa aagataaata aaattaataa atatatcatt gtttagattt tagtaaataa
     7321 ctactgaatt aaaagttgtt atagaaagtt atatatctaa atgatggtat ttttttgttg
     7381 agaaaattga aaagtttaat tgagatagta attttgagtt gacaaaaacc acttttaaca
     7441 ctactattat aagtggttgg taaaataata taaattaatt taagtatagc ataaaaaaca
     7501 aaaaaagtca atggaaaaaa taaaaaagga gaatttaaaa atgatatcac ctattatttt
     7561 gagttctata aatcagaatt taaaggaaat agaaagaaat gaactgcttg aaactaatat
     7621 agaatctgga gattatggtc tagctctttc tgaatctgat gtaaaagata taataaattc
     7681 gagagataat actttaaagg ggtatggaag aatagaatta gatataaaag ttacaaaaca
     7741 gttaatagaa aatatatata catcacaata tacaaatgtg gacaattatt tagaggctat
     7801 taatgatatg caagaaattt tttattattt aaaaaatgaa actgatgaca agatatgtga
     7861 tgatgagatt atagaaatac tgggtgaatt ttatgaaaaa ttttcaggca atatggacaa
     7921 tgtaagagga gaagcagacg aatttgcaaa gaaatttaaa tttggagaag tttaatatat
     7981 gataggagag attttgtatg ttaaataatt taagtaatat taatataaac gaaaataatt
     8041 taataaagaa ccaatattcc atatctctac ttaaggaatg tctaaattgt aaagtgatag
     8101 atgaaagaga agtatataat atacaacaag aaatatctct aatacttatg gatttgatta
     8161 aaaaatatac aaatggtcaa agcacatcag taaaaacaga agttgccgaa aaactgttga
     8221 tttcaatatg gtatgctatt gatgcatata taaataagct tggggatata gaaaaaagag
     8281 ttgtggagtt gactgctaag aatgttaaaa aagtgtacct tggaggcatt gaaatattag
     8341 aggaagagtt agttaggcta aaagattttt ataaactaat gatgagtaga aaattggata
     8401 ctaattcaat agcatataat gataccttga tggagttatc aagtttcttt aaaagttata
     8461 ttgtaaaatt tgaagctcag gacgttccag taagtataga ttatccattg gcactagatg
     8521 atatggatgt taagggtatt tattatgtga agaattatat tgagagtata gatatagaaa
     8581 atagattttg taacctattt aagaaaaaag atagagaaaa attattttat gactatgggg
     8641 tgacttataa aatagattgt gatgctatac ttgtaaattt atttgaactt attataaata
     8701 attgtatttt ttctactatt ttaggaaatg aagtgactaa tttagaaatt tcaaaatatg
     8761 agtatgaatt tttagaaaaa caatttaaaa atatgtacaa taatattact gaagataaag
     8821 ttatagactc tgaagaaagt aatataattg aattgattga agtagatgat agttttaaca
     8881 ctgaagtaga tagtaatttg gattataatg attttgaaga aattaacaga gaaattagaa
     8941 taaataaaaa aataacttta ctacttatgg aagcaattga agtattaata aaaagattaa
     9001 gcatagaaga tgaagatgta ataagatata ttaaaaaata tgaaaagata tttattgaat
     9061 ctgtgctaag ctcaataaaa agtaacactt ttaaaagtat ggtaactgtg agtaaaaata
     9121 aaaaggaaca cttagaagat tatattgttg atgatgaaag taaattggat gatgaatcgt
     9181 ttagagagat attcaatgag atattagata gtacatctat aattgaaaaa ataagaataa
     9241 taaaggaaaa tatcaatgct aaaaaagact ttatcgatat tcttgaatct gaatgtttat
     9301 ttggagaaga gtatttaatg ttgtttgcat cattaagtga attagaattg gcaatattgg
     9361 gtagtgtagt attttatgaa gatattagaa tgagagaaat taatatatta gaatttatgt
     9421 taaatgagaa aactgaaact actttatgga agatagagta cattaaattt atgaagaggc
     9481 aaagtgaaga taagatatac tccatagaag agcatatgaa taaaattaat taacaaaaaa
     9541 ttgtgaataa atagaatgtt agtatataat ataatctgta aagcatatca aaaatacata
     9601 tatttggagg aaaattatga aagagtatat cataaataat gttgaagaaa taagagaaga
     9661 attaattgat ttatctaaga aaatatggga aaatccagaa ttagcctttg aagaaaagta
     9721 tgcaagtagc attcaaaaag aatacctaaa gtcaaaagga tttaaaattg aagaagtaga
     9781 aaatttacct actggattta tagcaagttt tggagagggt aaacctgtga ttggcatact
     9841 tggagaatat gatgctttac cagagctatc acaatgtgta agtgcagaaa gaaagccatt
     9901 ggtagaagga aaagctggac atggatgtgg gcacaacctg ttgggtgtag caggtgtagg
     9961 agcagttgtt tctataaaaa aattaataga agaaaataaa tttaatggaa caataaaata
    10021 ttttggttgt cctgctgaag aagaaggtgg aggaaagaca gctatgtgta taaatggctg
    10081 ttttgatgat gtagattgtg catttacatg gcatcctttt gatataaatg caccttggag
    10141 aggtggaagt ctggcaaact tatctgtaaa atttaaattt aaagggataa cagcccatgc
    10201 agctcaagct cctcataatg gtagaagcgc acttgatgct gttgaaatta tgaatgttgg
    10261 agctaattat ttgagagaac atgttattga ctctataaga atgcactatg ttataactaa
    10321 tggtggtgga agacctaatg tagttcctgg atttgctgaa agttggtact ttattagagg
    10381 taaaaaagca aaggatgcag aacacgttct tgatagatta ataaaagtag ctcaaggagc
    10441 agcaatgatg acagaaactg aaatggagta taaggttaca gatggaatat atgactatat
    10501 accaaaccaa tgtttgactg atttagttta tgacaatatg gtttttgttg gttgccctaa
    10561 aaatactcct gaagaagaag aatttgcaaa aaagttgtgt gatactctta ctagagaaga
    10621 aagattaggt gtagcaacat cattacaaaa tgataaatct attgtagaaa gctatataca
    10681 caaggaaata gttgacggtg acaaagataa aggactagct ggttctactg atgttggaga
    10741 tgtaagttat gtaataccag tggctcaatt tgcaatggca gcttggcctg ttggaattgc
    10801 aagtcataca tggcagtcat gttcatcagc aggttcaaat ataggatttt ctgctatgat
    10861 aaattcagct aaagtactgg cttgtagtgc ttatgatgta tttatggata ctaaaattat
    10921 tgatgaagca aaaattgaat ttgataaatc attagatgga caaaaattta aaccattggt
    10981 ataattttgc taataaaata tttctggggg gaatagctat gagagaaaaa gttgaaaaag
    11041 tgcttgagga aaagataaaa ccagtattac aaagagatgg tggagatgta gagcttatag
    11101 atgtaaatga aaatggtgtg gttttagtta gactacaagg ggcatgtagt gggtgtccag
    11161 gagcgactat gacaatcaaa gctattattg aaaatgtttt ggtgagtgaa gttccaggag
    11221 taactcaagt attaggtgtt taatagttag cgtttattaa atgtatgtta tacataagta
    11281 tttaataaag aagtaaaaaa aatagtgaag caatatatat gaaaaagtat gagaaagata
    11341 ataaaacatt atagaaataa tgaaatattt catatgtata tgaagaaa
//
LOCUS       DAMJWU010000110        11195 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMJWU010000110 DAMJWU010000000
VERSION     DAMJWU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.114"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(21..653)
                     /locus_tag="PC460_003111"
     CDS             complement(21..653)
                     /locus_tag="PC460_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2601976.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(725..1078)
                     /locus_tag="PC460_003112"
     CDS             complement(725..1078)
                     /locus_tag="PC460_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2601977.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1156..1359)
                     /locus_tag="PC460_003113"
     CDS             complement(1156..1359)
                     /locus_tag="PC460_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2601978.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            complement(1749..1979)
                     /locus_tag="PC460_003114"
     CDS             complement(1749..1979)
                     /locus_tag="PC460_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2601979.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1981..2388)
                     /locus_tag="PC460_003115"
     CDS             complement(1981..2388)
                     /locus_tag="PC460_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2601980.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            complement(2748..3197)
                     /locus_tag="PC460_003116"
     CDS             complement(2748..3197)
                     /locus_tag="PC460_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2601981.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(3211..4137)
                     /locus_tag="PC460_003117"
     CDS             complement(3211..4137)
                     /locus_tag="PC460_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601982.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4262..4666)
                     /locus_tag="PC460_003118"
                     /pseudo
     CDS             complement(4262..4666)
                     /locus_tag="PC460_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            complement(4816..5436)
                     /locus_tag="PC460_003119"
     CDS             complement(4816..5436)
                     /locus_tag="PC460_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2601983.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5535..6803)
                     /locus_tag="PC460_003120"
                     /pseudo
     CDS             complement(5535..6803)
                     /locus_tag="PC460_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6895..7710
                     /locus_tag="PC460_003121"
     CDS             6895..7710
                     /locus_tag="PC460_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDF2601984.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSSRAEMERL"
     gene            8382..9149
                     /locus_tag="PC460_003122"
     CDS             8382..9149
                     /locus_tag="PC460_003122"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2601985.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9477..10154)
                     /locus_tag="PC460_003123"
     CDS             complement(9477..10154)
                     /locus_tag="PC460_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601986.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10304..10621)
                     /locus_tag="PC460_003124"
     CDS             complement(10304..10621)
                     /locus_tag="PC460_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601987.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10643..10981)
                     /locus_tag="PC460_003125"
     CDS             complement(10643..10981)
                     /locus_tag="PC460_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601988.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3702 a         1783 c         1615 g         4095 t
ORIGIN      
        1 tataaataaa ttataaaata ttaaactaat ttatcaatat ttcctgattg aatatgctta
       61 atgtttgatt gaattttttt atatggccaa tcccaccatt taatttctaa taattttgaa
      121 attgtatcat tattaaatct cttttttata atttttgcag gtactcctcc aacaatagaa
      181 tacggtggaa cgtcattagt aactactgct cttgttccaa taattgcacc atcaccaata
      241 tttacacctg acataattat agaatcataa cctatccaca catcatttcc aattataatg
      301 ttacccttat tgtcccaagc gtccgttata tggttgatat cgagtttcca ttcttcataa
      361 aagatgggaa aagtatatgt ggattttgat ttcattgagt gatttccact agtcattaga
      421 aattttgcat tacatgctat tgagcaaaac ttaccaatta ttaatttatc attgtttata
      481 gggtaatgat acaatacatt gtttttctca aaatctcttg ggtcattata aaagtcatta
      541 tatattgtgt aatctccaac ttctatattt tcccttgtta ttacattttt aagatataca
      601 gtttgataat cattacttct tggatatacc ttatttgaac ttggaatagt catatttaaa
      661 tctctccttt aatatatatt atgtttttga ctattccact actacaatac atctcttcac
      721 agaatcactc cttgttaaga atcttcataa gataatggcg tcctttttta gtgttaagtc
      781 ggatattctt tcttagtcca ttctttttag cgtataactg acaaactgtc attttcttac
      841 caatagtgtc aatgctatca tcaaggtcat tttgtattgc tttttcctta tcccttaatg
      901 atatatcatc acgttttccc tttgggggtt tatcagtagg tacaagtttc caagagtaaa
      961 caaattgtgg tttaccaaat gaatatacat acttatatgc gtatcttcca tcttttctct
     1021 ggctctctcc agttcttaaa attctatctt tattatctcg tcttttttca gttgacatga
     1081 taagaagctc ctctcaataa ccgaattgag cttgatacgt catatgaata ttataacata
     1141 taaggctcaa tttcattaga taaagtcaag tgtatcaacg attttctcaa attgttttct
     1201 ctttatttga atgcgattgc tattcataat gacccagcca gaatcaaggt tttcatcagc
     1261 aagtttacgt aattttttct ctccaatacg aaagtatttt gacgcttctt caatcgtcaa
     1321 ggtatatttt tcccaaatag gtacatcagt attgttcata gaatgtatca ctcctttcta
     1381 tgttcgtagt tcagaagtag atagatggct ttggtaagct ccattggtat tttaaccaat
     1441 cccattctta tgggcagaac cgtgtcatac aggtgtatca ttatactgtg gtacaggtta
     1501 cggcaaaaca accgttccaa aggttattca caaatcacta tatagtagct ctcttgatta
     1561 aggtcatggc gtatagtatt tgttggctat ctgtatcaca aacgtatcta tctactttat
     1621 tcagttgtca aagaacagtc ggcattgatt tgtctataaa aaatcacaat agcttatggg
     1681 cttttataga caaataatta gtgattctcc atgtaccaaa tagcttgata catggagaga
     1741 gggcgagatt aagacaaatt aaatctcaaa atagcagtga ttagttttgt tcctagcatt
     1801 cgctttgttt cttcttctac atgaatatag ggatttccgt attcatcaaa taaagttttc
     1861 tgtgaaagac gaatgatata gtgttcaaaa tgtttaagta ttccgtttat tgcatttaca
     1921 tcgccatgta cagcaagtaa aataattgta aaaggtaata cttctttagt aggtttcatt
     1981 tcaatgttcc tccatgagtt ctttcaactc ttttagtgca tttgttttgt gataatgtat
     2041 ggtattttct gctaaatcca tcaatgttgc tatgtcagca tttgtcatgt tgagaaagaa
     2101 tgaaagtaga attatatctt gttttctttt tgtaagtttt ccgattgctt gtgccattaa
     2161 tacatcttca attcgtatgt tgtagtcgta agaaaccata cagatgtagt tagaatcata
     2221 atcatctaaa atactcaatt tacttaattc tgcaagtgac aaagattcga gagataccaa
     2281 ataatcattt ctgcgtttat tttcgtcata aatatctcta gcatagttac gcaaaactgt
     2341 tttacagaaa ctgtcgaatt gatgttcaat tgtcttttca tcaaccataa gatttcaccc
     2401 tctttccatc gcaaggagtt aaataacctt gcttactcct atcacggtgg gagggcgtta
     2461 aatcttcgat aaatttcaaa aaaaagagaa aaaaatacac actctgccta tagggcaaaa
     2521 atgtgtattc tttaaaacgt atttaattta tattggttgt aagccaccac catattgatg
     2581 tatggaaata tgcattggct agcttttcat aaaaatacaa agatgcataa gaaattaata
     2641 gaggggaaaa acttattctg ttattaaaaa gtaggttaat tctaataaat gctattgcgg
     2701 tcgaattaaa ctatatacat ggcaatcaat catgtttcta ttctttatta tgaattgtga
     2761 cagtattctt caatcttaaa accacatttt tccaatactt tacttgaccc tctattgtag
     2821 gaatatacat tagcatgaat tttgttaagg ttgtattttt caaaggcaat tttgcatatt
     2881 tcttttactg catttgttgc tatccctttg ccccaatagt tcctagcaag ccagtaacct
     2941 aattcagcat ttttattaat agaggtatct tcttcaaatg ttaggctgat acttccaata
     3001 gccctttctt gataaaaaat agcatagcta agttgtttaa catctggagt tgccaaacaa
     3061 aaactaacaa aatctctagc gttttgctcc ctataagggt gggggaagtt gtcacgaaga
     3121 ttctttgcaa tttctttatc attcgcaaac tcaactaaat cggtaatata agcataattc
     3181 cactctataa gttccatttt tcttctcctt ttatgaaaca gctacaagaa atctttctaa
     3241 gccaatacca cttatcaaac agcgttcttt tttatttcca agcatttgat aagtccagtc
     3301 aacaaatcca ccgtcaacta tttcaacggt ttcatttcct tgtttgaccg ataatttgaa
     3361 attgatacct ttataataat tattatcaac atcatcatta tgaattgata actctatatt
     3421 aggaaaaata agtttgatag tttcaaccat tctgtcgaaa aatccgtcat tatctttgta
     3481 tccgtttctt ttgcgtagaa taatagagaa acgaacattt tctatttcac ttaatatatc
     3541 tttataatag tttaagtgct tttctaacaa cattttttca caagtgtaag accctgtatc
     3601 tataccagat gaaaccatac aatataagcc aaaatgtgaa tataaacctt tacctacaaa
     3661 attttgtgct cttattaccc tagcagtagt agccatgtgg ataggtatgg tattattttc
     3721 ttcttttctt tttaatttat cagcaataat tattgccaac atatttgagg ggtctgcaag
     3781 tgtttctgtc cctcgtagag cacttactac attatattga tctacggagc caaaaacgct
     3841 acagctacct aatggagcag agggagaaag cataatcgtc ttgatatcca tattgaatgc
     3901 ttttctcaat aattttgact ccaaagcatg aatttcttct ggatctatac cgctagggat
     3961 agtaaatcta tttagttggt atgactttag aatatcagtt gcagttaaag tatttgcttg
     4021 tctatcaaac acttctaata gaagtgagtt caaatctgat tttgatagag ctgataactt
     4081 atcaactata ttcttatccc caattttatt tgtaattcta cttaaaatat cactcatagt
     4141 tatcctccat actgttactt tcaaaataat tatagaatgg ttacaacaac taatctaggt
     4201 catttgacca aaaatgaaaa aacactgaat atttactatt caatattttt ttactttggg
     4261 tttatttgat taaatcttga aaatactgta atgcaaccct ttcagcttca acctttcgga
     4321 caataggact ttgtgataat tcctctctta actcaagata caggttgata gtagcctgca
     4381 atgcattaac acagctttta tattcatggt tagcaaccgt cttttctaat gcttcaatat
     4441 attggggtgc gtccaattca atttttctaa ctccacgagg taatttacct aagagaagct
     4501 gtattaatgg ggcaagtact gattgtctta aaaaagataa aaaatcaatt gtttcaaata
     4561 gttctctacg tccaatctta gtgcaagcgt aatgaaccca aatccaaaat ctatcttcaa
     4621 tccactgtaa gtctggagtt gggaagtatg cttctttttt agtataacac ctctcacttt
     4681 cttttattag ctatcaatcc aacataatta tgcataggag tattatataa tgaaaaaata
     4741 aaaaaaaata ggtcacttga cccgaaataa aaaagcattg aataattatt attcaatact
     4801 tttttgaaat tatacttatg ttgtaggaag attctttaat acctctcgct gattaattac
     4861 actaaatctt ttgttttcat aagcaattag atgttcttgt acacattcat ttatagcacg
     4921 attaatgctt ctaattggag cgtttacttc gctagcaagc tgttttttgg taagcagatt
     4981 taattcactt ctataatagt gtaaggatat acacctcaga agtcgttctt taacacttaa
     5041 agatgataat tcttcaatta attcagcatt tacaactaat ttactagata tttctcgaat
     5101 cagaaatttt gtagcttcaa aatctcccct taaccagttt aaaaaatcat ctctatgaag
     5161 tttatataca ttacaatctg tgaaagcgac aatttcaacg ggttttttcc catcattaaa
     5221 ttgttccatt tctccaaaaa aactaccact tctataaaga tgaatagttg agatgtttcc
     5281 tttcacattt aagatgaatg cttctgcaat accgtcaatc ataagaatta catattcatt
     5341 ttcttgctca gcaaaaagaa tagtctcttt ctttttatac gtttttttaa caagcttctt
     5401 tttaatatct tcattcataa aatcaattaa atgcatattc atccctcctt accaaatctt
     5461 tgattaaatt atatcatctt tcaattagtt aggtaagata gtaaaaatat atttacatag
     5521 aaactattcc attattattt tccagttgtc atttttctgc aaagtaagct caaactgaga
     5581 aagctgttga caatagaact agtaggaata tatgagcgaa ccatttcagt attcaactgt
     5641 tgtaggtttt cagtcagata attcttcaaa cgttcaattc gtttgtcctg tgaatcttgc
     5701 gatgctctaa ccagtttcat agatagcgtt ttaccttgtg ccacctgcaa aaaagaccat
     5761 gcttcgtcaa ggtcaactat cttgaagcta gagcggtcag tatgaataaa gtctaatgca
     5821 aaggtactaa taacaatcaa catagcaaca ctaagaagtt ctattgttgt atattcttca
     5881 aagctagtgt tggcatctgg caatacgagg tcagcaactt gaataatatt cagctgtttc
     5941 tccaagctga tagactgctt cacatttccg tcaccaaaca gtagatgtgc aaaatcatag
     6001 tctacaaaac tttcgatatg gtcggctatg ctgttactga tagaagtatc ttcttttcgt
     6061 aattcatcaa tgacaagtaa taaccctcgc tgttcacttt gggttacatt gcggattgct
     6121 tttctaagca ctgggaactt atcactgtca cgactggaga taccagcaag gaatgttagg
     6181 atatcaatcg ctaaactttc agaatcattt ttacggctca aaatgacgta agggtcaagc
     6241 aatcctttgt tttcatcatt actggttaaa ttgacaatgt ttatctcatg ggctatttct
     6301 ggtagcgttt ctttccattt gccgcgttct gctttagggt caactatgac cgcctgtccg
     6361 ccgaatagta ccgcatagta aacaagcata ttgttactga ataatttacc accacccaat
     6421 gaaccgataa acgcagacgc taacacattg gtaatagaac cctttaccca ttgactagca
     6481 agggcaggtt ttagatagac attctttcca gtgtccagat tgtagccaag atagatatcc
     6541 tcatgttcgc caagggtttg tgtcgctcca aaaccaagtc cagccaaaaa atccgaagtg
     6601 acatattgaa tataatcatt gatgtaacgt ttgctgacag gaataaattc gccatgcaat
     6661 cccaacatat ccccaaaggg acgcacgagc ttgagtgata agtcgtcgta aaagtctttg
     6721 acttcatcac aacgcctttt tagctcgtca agatcctttg tagatacacg gacaacatag
     6781 ctaagtttat acatagattc tttacagtta aaagtctgtt tttaaaaaaa tgtataaatt
     6841 ataaatcaaa atatagatac tatcaatgac ccactaggtg caaaaagcat tgatatggtt
     6901 aaattatcag acgctaaaag ctgggctatc cgaatgagtg aagatgggtg tgcttataaa
     6961 tccattgaca attataagcg ctcactgaaa gcgtcgtttt tcatggcaat tcaagacgac
     7021 tgtattagaa agaatccatt taattttcag ttaagtgatg ttttggaaga tacaacagaa
     7081 ccaaaaatcg ctctaacacc agaacaggaa atgggcttat tatcctttat ggagagcgat
     7141 aagacttatt gtaaatatta tgatgatgtt gttatcctct tagaaacagg actacgcatt
     7201 tctgaattat gcggtttgac tttagaattg gatatgaaaa ataaagccat tatggtagac
     7261 catcaattgt tgagtgatac taagattggc tattatgtag aagttccaaa gaccacacag
     7321 ggtaagcgag aaattccaat gactgaaaga gcttatcaat catttgaacg tatcgtggaa
     7381 cggcgtaaga acgctaaagc agaaccaatc attgttgatg gttaccagaa cttcctattt
     7441 cttaaacaag atggattacc aaaacaagca actcattatc aaagcatgtt aagagcgtta
     7501 gtcaagaagt ataataagac ccatgaggac aagctaccaa agattacgcc acataccttt
     7561 aggcatacac tctgtacaaa tatggctaac aaaggaatga gtcctaatat attgcaatat
     7621 attatggggc ataaaaacat tactatgacg cttggctatt atgcacatgg ttcatttagt
     7681 tcttcgagag ctgaaatgga acggttataa tcctagtagt atgactagag tttactactt
     7741 atttactact tttgatggca ttcttatacg gttaaatgcc aacttatgca aagtatcttc
     7801 caaaaagaaa atgccgatac tccttgtaga tacaggatat atcgacattt accaacttat
     7861 gaaaagataa tcaaatttaa tataagtttt tcaaataaaa agataccaaa ttctttttcg
     7921 agaatctata taacattact taagcttggt tggggtataa acaattgagt agctactttt
     7981 gtatggtgtt caactttagt caaaactctt aagtaatgca aatgatttag attcattaat
     8041 attcaatctc ttcataagta gtgaaattcg agttatttct gcataattat actatgattt
     8101 atagattaat tctattagca tccagaataa tattgtttat atgagtaaaa tttaaagaat
     8161 attgtgaaaa ttataggatg ttaaaataaa tttaggtaaa aaagaagtgt ttgtatatta
     8221 tagagtacct tttctaaaat atgtgggatt gttttaagaa ttagaataaa aattattttt
     8281 aaattagtat ttttataatg tatagtattt ttaatgtata attaaattgt gcagaaaata
     8341 gaatttaaag tcctatagaa tttctgatta tggaggagaa tatgaaaaga aaagttcaag
     8401 taaaaaatat tactattgga gaaggtagac ctaaaatttg tgtaccaatt attgggaaaa
     8461 ataaaaaaga tattataaaa gaggctaaag agttaaaaga tgcatgtttg gatattatag
     8521 agtggcgagt tgattttttt gagaatgtag aaaatattaa agaagttaag gaagttctct
     8581 atgaattaag aagttatata catgatatcc ctttattatt cacatttaga agtgtagtag
     8641 aagggggaga aaaattaata tcaagagatt attatactac tttgaataaa gaaatttcta
     8701 atacaggttt agtagattta atagatgtag agttatttat gggagatgag gtcatagatg
     8761 aagtagttaa ttttgctcat aaaaaagaag tgaaagtaat tatatctaat catgatttta
     8821 ataaaactcc aaaaaaagaa gagattgtat ctcgtttatg tagaatgcag gaattgggcg
     8881 cagatttacc caaaatagct gttatgccgc aaaatgaaaa agatgtgtta gtgctacttg
     8941 aagctacaaa tgaaatgttt aaaatttatg cagatagacc aataataaca atgtcaatgt
     9001 caggaatggg tgttataagt agattgtgtg gtgaaatatt tggttctgca ttgacttttg
     9061 gagctgcaaa aagtgtttca gcaccagggc aaatatcatt taaagagtta aattcagtac
     9121 ttaatctatt acataagagt attaattaaa ttaattatat agaaatatta gacggtattt
     9181 ttgaagatat aaattaaata taaaaaactt ttcagatagt aaaatttaaa ttgggcaatt
     9241 atatctgaaa agcaactatt tagtgtgtat tttgaaagtg atatagaaaa tcttataaat
     9301 caactatggc atatttaaaa tatgccatta aaacctacta gacattagat tatagatggt
     9361 atactagcat ttctattatt tatagatagt aagttttcat aataactgtt ttatctaaag
     9421 tagaagatat atttgtatta tgtatatttg tcattatttt tttgtaatta tgtatattaa
     9481 tggttatata tgtaatataa tattatcact ctattttttg tatattgtac attacaacat
     9541 ttatatctat catcatcaat tacagtagaa tgtatgggat acttattttc acttaaccaa
     9601 tatttttgca tattctttgt ccaagacttt ggaaacttca atactttctt gtttattata
     9661 acatcctcat cttcaaaaat attaacttct ctttctattt ttatttttct gttgtatatc
     9721 ttattgatag attctttgtc gagttttatg tctctataca tatcagggca atagcaacaa
     9781 acataatttc ttttttttcg acgtttaaat ttcacaactt tctctatgct aatttctaat
     9841 ttttttgtac catcttttat aaatttaata tcttctatgt tctcgcttga aaaaatttct
     9901 gtcaatctta aagtatctac agtttctcta attatttgct ttttataaga agagaatcca
     9961 tatccaaaag aaaggtttaa tttattaggg atgtagccat ttttatattt aaacatctta
    10021 attttttcca attcagcatt atctttaaat ttcaactttt catacctaaa agttactttg
    10081 ctaaaaagag tatattctac atcaatatat tcttttgcaa tcttaaattt atcgctacct
    10141 tcaatcacaa tcatagccta tctccaatcg ttttatatta aaataaggcc atagaacatg
    10201 gtctaatagt actcattagc actataagac aatgttaaca caaaaagaca aaaaaaggtt
    10261 atttatttca aataaccttt ttttttatat aaaatataaa gaattatatt ttattctttt
    10321 ttatgatgtc tataatctgt gatatcagat ttagttctct tgaatttaag tcttttaact
    10381 cttcatttat atcattttct aaatatgttt ttccagtgga agtaatatct tttataagaa
    10441 catctgcaga aatatttaat atatttacaa attttatgaa tgttttaaga cttggattct
    10501 ttttaccact ttcgatttga gttatatacc aaggcgatac tccaaccata tttgcaaatt
    10561 cttttgaagt taaatttgct tttttccgtt cttctttaag tcttttacct atataattca
    10621 ttattgttac tatttccctc acttatattt ctttgtattt aatgatgcac tttattaact
    10681 ctagcattgc aatttgttct ttttcagtta atttattaat catttcataa cattctaaag
    10741 gtacagagtt atcaacgcct tctgttgtaa taatatttgc aaataacaat tcaagtggta
    10801 tgtctaaagc agtagcaatt ttcattataa tattgataga tgtatttgtc tcagctcttt
    10861 caatctgacc aatatatgtt ggatgtaaac ctgccttttc tgctagaaac tcttgactat
    10921 atcccaattt tgttctatag acttttattc tatctcctat tatttttcct atatttgcca
    10981 attattatcc ctccacttta gattatattc atagtttaag ttgttttata tagactattt
    11041 atactttttt agtctaaata tagactatat atgtttttat actgaaataa gtgctattta
    11101 tttttgtata gtctgtatct acaataaaca aatatggata aatcatattt ttaattagag
    11161 aaatattttc ataatttcat aaccatacta caata
//
LOCUS       DAMJWU010000111        10904 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMJWU010000111 DAMJWU010000000
VERSION     DAMJWU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.115"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(361..945)
                     /locus_tag="PC460_003126"
     CDS             complement(361..945)
                     /locus_tag="PC460_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2601989.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSLDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
     gene            complement(1267..3111)
                     /locus_tag="PC460_003127"
     CDS             complement(1267..3111)
                     /locus_tag="PC460_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2601990.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            complement(3406..4368)
                     /locus_tag="PC460_003128"
     CDS             complement(3406..4368)
                     /locus_tag="PC460_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF2601991.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            4559..5110
                     /locus_tag="PC460_003129"
     CDS             4559..5110
                     /locus_tag="PC460_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2601992.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            complement(5649..5936)
                     /locus_tag="PC460_003130"
     CDS             complement(5649..5936)
                     /locus_tag="PC460_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601993.1"
                     /translation="MKKILYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFMTIKIFIFLSLPLCFNILFNYLTS"
     repeat_region   6345..7429
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6411..6439
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            8076..9347
                     /locus_tag="PC460_003131"
     CDS             8076..9347
                     /locus_tag="PC460_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2601994.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     gene            complement(9987..10742)
                     /locus_tag="PC460_003132"
     CDS             complement(9987..10742)
                     /locus_tag="PC460_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HDF2601995.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT         4010 a         1604 c         1115 g         4175 t
ORIGIN      
        1 ttttgattac taatcaaaag aaaatatcta agtaaaataa aaattatatt tattaagctt
       61 tatgtattat agtattttat tcttacttaa tcatcatact atagtaaaat actaacctag
      121 aagttagtat tttacttaaa aataagtgct tattcaaaca tcaaatagaa tatatactat
      181 tataaataaa tctataaagg aatgactttt ataaaaaaat ggtggaaatg taagatagaa
      241 gtgataaaaa ctacattcca caaaaattta acaaatgaat atggatgtga aagtattaga
      301 aaatacccta tgtataaagt aagaaaggtg ttttttgaga ttttgcaaga aaatttaact
      361 ttatttattt tctcgaataa tacggcatgg atttccgtat gcaatcatat tacttggaat
      421 atcttttgta acaatacttc ctgcacctat aactacatta tctccaatag taacacctgg
      481 catgataata gcacccccgc caatccaaac attatctcca atagtaactg gagctgtttg
      541 agttttacaa aattcaaatg aaccatcttc tttggcatta ccaaatctat caccagcatt
      601 agttggatga aatgcagtat atatttgtac gtttggtgcg attagtgcat tatccccaat
      661 gataatttta ttatcatcta aaaacgtaca attcatattg acttcacaat tatttccaaa
      721 ataaatatta tttccataat ctacataata cggtgcagta atccataagt ttttaccctt
      781 accaccaagt aactcattta atattttttc ttttttttcc aaatccaagg aatctgtttg
      841 gttataatct cttactaggt cttttgcttt atgccattga gttaacaact caatatcacc
      901 acaatcgtaa agttctcctg ccaacatttt ttctcgctct gtcatgcttt taaccctcca
      961 aatctatatt gtttttcata tatattctag cataagattt atatatctat atattttact
     1021 tatataaaat ctaataaata ataaaattta ttaaatatac agttgacaat ctacctctct
     1081 atcatattaa aactctttta attgtaataa tgtcatagca atttttttac ttttattcct
     1141 tatttatatc tgataaacac aatttttata agaatagcct taatttaaca accttattta
     1201 tgttaaaaag agatattatt gattttaata atatctcttt tactggataa tacaattata
     1261 gaatatttaa aataaataat ctgacacttg actaataaat ccatttttct tccaataaat
     1321 tgcactctct ataaggtatg gattatctgt aataatacta tctacattta ggtcaagcat
     1381 tttctcaatt aaactgtcac tattaacagt ccaaacgaag atttttttgt tcattttatg
     1441 aatcttttct actactgttt tattaacaaa agttgattca attccatata tatcaacata
     1501 atttaacttt gaaaaatcac catatgcaat tgccataaaa tagcatgtca gtaaattctc
     1561 atccagtttt tttacttttt gtaatatagt tttatccata gatgcaacta tacattgatt
     1621 ttcaaaatta ttttctttaa ttaactgaac aacttttttt actacgtctc ccttttcatg
     1681 tccattaagt tttatttcta ttaataattt caccttacct ttagaatatt ttatcatttc
     1741 tttgagtgtt ggtatctttt ccccaacaaa cttttcatca taataacctt ttccataata
     1801 ataaaagctt cccgcatcat atgtctttac ttcattataa tttacatccc aaacattttt
     1861 atctactcct gtagttctct taaagtttga atcatgaagt acaataagtt ctccatcttt
     1921 agtttcttga acatctattt ctgcataatc agctttagca tgtgaagctt cttctattgc
     1981 tgccaaagta ttctctggag ccatttcagc tcctgctcta tgagcaattg ctgttgtata
     2041 aaatgaaaat gacgtattaa acataccatt tatatgtgaa gaaaatgcaa ctgactctat
     2101 ataagaaatc aataatatta atactaaatt aaaaacaatt tttgatatag attttttctc
     2161 ttttttaata ttatttttag aaatatttac tcctttatat tgataataag ttgctgagat
     2221 aaatccaatg tccaatataa aaatgaatat tgataaaatc aatctaaaat attcttcaaa
     2281 tgcaatacat ctatttataa atacattctt caaaaattcc atatcagtac aatatttagt
     2341 ccatatgcct ataaatataa ttccactatg ataaatcgca tatccaataa tagctactac
     2401 taaagataat attaatgaat aaataattgt tcttataatc tttcccttta taagattaac
     2461 actttctttt cttgctcttt taaaattttc tgtatttaaa gttatctcat gaatgctaaa
     2521 aatccatcta ataaccgaaa tatatattat taaaagaaaa atcatataga ttatatttaa
     2581 aattatatca gactttatat aatctaatat gtactctggt atttgtattt ttcctataaa
     2641 ccctgaaatt aaaactgtat ttactaatgg aattattaat atcacaaata taaataatag
     2701 tatattttta ggatataaca attttataga gttctcaaat gatattttaa agagttcaaa
     2761 taaccctatt ttttcatatt ttatcgattt attaaaacaa ataattacag aggtaaattc
     2821 aaaaaatgca taaaaagcta gtattataac tgatagtatt atcagaacta ttgacatggg
     2881 attggtcaaa acttttataa aattatccat atttaggtag actatatgta aactttttat
     2941 taaaatatca aatatatatc taatagatgg ccatataaaa aataaggcta atcctttatg
     3001 aaataattca aataaaatta atgtactaaa attatatttt attataaaaa caagtttact
     3061 taaaatattt ttacccgtaa atataaaatt tcttaaaatt ctcttttgca tttttatctc
     3121 cttgtatcta tttgttctaa ttttatcata tattttaaat acatttagaa cattaatatg
     3181 tattattcca agttatgatg tataaatgca aaaacacata ctctttttta ttgcatagct
     3241 aatcatcaat atctatatag cttaatactt ataagtagga tactccatat ttacaaaata
     3301 aaaaagtatc tcctttataa ctttcaaaat ttacattgag atacttttta taatttaaat
     3361 ctatatactt aatttaaaaa acattgcata actattgtgc tattattaca attcttcata
     3421 taaatcagta cttaaatatt ttaaaccact atcattaaga gttaaaacta tattttttcc
     3481 tttttcactg ctctttaaca acttaattgc tgcacataca tttgcccctg aagaaaatcc
     3541 tgcaaataca ccttctaatt tagctaattt tcttgctaca tcagttgcct cctcgtctgt
     3601 aacttgaata taaccatcaa taagtttctt gtctactaat ggcaaatcca tagaatatcc
     3661 tccaccttgt attttatgcc cttggtcagt tatttcttta cctgaataaa ttgctgctgt
     3721 ttcaggttca acaacataac atttgataga agaattatgt tttttaagtg tttttgaaca
     3781 tcctgcaaaa gtactaccac ttccaagaaa atctacaaat acatcaatat ttccatccaa
     3841 ttgttcccac atttcttctg ctgtatactt ttcatgtgca tgattacaag cttctaaatt
     3901 aaattggtct gctctaaatg catttctttc ttttgctatt ttttgagcct caatttctac
     3961 aagtgccaaa tcttcaccag atacctgtcc atgaactgaa ttaggcattt ggtctacaag
     4021 aactacttca gcacctaaag ctttcatcat tcttgctctt tccatagaat ttccttttga
     4081 catacacgct ataaatttat atcctttaca tgcacaagct attgctagtc ctgttccagt
     4141 attaccactt gttaattcca caactgcttg acctggaact aataaacctg cattttctgc
     4201 ttcggtaatc atttgtaatg ctacacggtc ctttttacta aatcccggat ttaagtattc
     4261 catctttgca taaatattac cttgaactcc aaattcatct gctattttgg ataaatgtaa
     4321 catatgagta ttgcctatag cctctgtaac attattaagt acacgcatta caattccccc
     4381 ttaatctctc aactatcatc cgttgaaaaa caactcttta tgcagtataa tttatattac
     4441 ggatgtacag tgtatttaac gttcattata ttatacattt gtaaattata aataatgtta
     4501 aataaattat acattcgtaa attatatttg tcaataattt acattttaag gagctactat
     4561 gagtattaat actgttattg ctaaaaattt aaatagatta cgtaatgaac gtaacttaag
     4621 tctaggtcaa cttgctgaat tatctggcgt aagtaaagta atgctttctc aaattgaaaa
     4681 gggagactca aacccaacag taaacacaat ttggaaaatt gcaagtggac taaatgttcc
     4741 atatactgca atactggaac aacctcaaaa tgaaactttt attgtatcaa aaactgatat
     4801 agatgtacaa gtttcagaaa ataaagatta tcgtctttat tgttattatc ccaatacacc
     4861 aactagaaac ttcgaattat ttcaaatgga attagaagaa ggtcatagtt atacatctgt
     4921 tggtcattct gaaaaatcac aggaatatat tatgattata gaaggtcaat taaaattaga
     4981 agtaaatgac tctatttatc aacttagaga aaatgactct atttgttttt cagctgaatc
     5041 aattcataca taccataatc aaggagaaaa aacattgaaa gcagtaataa taaattatta
     5101 tccagtttag ccatgccatt taataataga ataattattt tctatacaaa aagataaatc
     5161 ctttgattag gatttatctt ttttatttta tttttcttta taaattcatt atatatcttt
     5221 acacttttat caattatgtt ataataagct tatataaaag ttttatattt ttgacaaatg
     5281 gagtacaaaa tactcctttt tcttttttac cattgtgata attatttagt tagtattaaa
     5341 atattatcac ttaaaacaaa tacaaaacgt ctcttatatg tacatttttc attgttgtaa
     5401 taatgatttg tgtatgtatt tctcacaatt ggagtgctta tgcactcttt ttaaatacct
     5461 ctattaccat aaactaaaag ctattttatt atccctttta gtttattttt aagtagttaa
     5521 ataaattatt ttatattttt ttcttcattt atatgaaact tttgttttat cactatatgc
     5581 actttatatt tactacaaat aacacataag cttactttta tcataagact cctatatcta
     5641 aatataattt aagaggttaa ataattgaat agtatattaa aacataatgg taatgacaaa
     5701 aatataaaaa tctttatagt cataaatttt atcttatcat ttttatcctc tccattaaaa
     5761 taattatctt gataaaatgt agctctattt tgataacctg tttttaaaac tccattttga
     5821 attaacagca atataaaaaa gattgctaat aaaataaagt tataattttt ttcttttaat
     5881 ctaaaattag ctataataac aatgaaacta tataaagcat ataaaatttt tttcataaaa
     5941 aacacctaaa ttctatattt gtattttata ttgagccaaa tctaatttct gtatatttat
     6001 taagtgtagt tctagttcct attgtagtag cattaaatat tttttaattt ttattttcaa
     6061 agggcatctc cttatattta aattatagta tgaatattca taaatttcaa tctatttata
     6121 aggtaattat tttttatatc taatatcttg tcattaagtt ttattatata tatttataaa
     6181 tttttcaata ctttttataa ttattctact tttatatttt aatcattaaa actaaagtca
     6241 atgttatctg gtaattattt actacaaatg ctaaaaaata attatatttt aaaggataaa
     6301 atgctgtata aattattggg gtattttacc tatacttgtt gcaaatttac ataccacgta
     6361 gttaatataa aacaagatgt ttttaaaaat aaacttaata tagatattga atttacatac
     6421 cacatagtta atataaaact ttaatcatat atcagatagg tcacaactta agagtgattt
     6481 acataccaca tagttaatat aaaacaactt ctcatattcc agataattgt tattttacga
     6541 aatttacata ccacatagtt aatataaaac ctgtagattt gcctgtttcg atattgctca
     6601 tgtgtgaatt tacataccac atagttaata taaaactctc ttgtttcttc tgttaatttt
     6661 attatacctt cattatttac ataccacata gttaatataa aacgcgttat attgttgtat
     6721 aaaaccgatt aaagttcaat ttacatacca catagttaat ataaaacttg caacatattg
     6781 ttttatcgtt gctgggtctt taaaaccatt tacataccac atagttaata taaaactaag
     6841 cgatattaga gagcgcaagg catagcggag caaatttaca taccacatag ttaatataaa
     6901 actttaattt taaccacccc ctttcactaa taaaaaaaga tttacatacc acatagttaa
     6961 tataaaacgt tgcatatgta tgtctaagag catgaaattt aattttattt acataccaca
     7021 tagttaatat aaaacttcta ttaagtgtaa gcaaaacgct tggaatatga aatttacata
     7081 ccacatagtt aatataaaac ttatacacaa atacaacttg aagaagcgct aaagagattt
     7141 acataccaca tagttaatat aaaacctaat gagtacatag ataaacgtac aggagaggtt
     7201 aatttacata ccacatagtt aatataaaac gttgataatt ttcgtttgta tactttttat
     7261 aatttaaaat ttacatacca catagttaat ataaaacata aagatttaga aaaagaaaaa
     7321 gttaaaattt tggaatttac ataccacata gttaatataa aacttttcct gttaagttga
     7381 tgtttgcaac tttaactgct atttacatac cacatagtta atataaaacc ccaaaataaa
     7441 ctaagtattt tcagtactta cacacataca actctctcaa atttgcagtg aaccatgagt
     7501 agtgcaattg ataacattta tcacacaccc tcaatccctt acattgcaac tgttaaaccc
     7561 tactttatca aaaatatcgc tcactgcaaa atttatatat ttttattata tcataaaatt
     7621 caaactataa aattaaaatg gaataattta tcaatttaag aacacctaca tatttgtaaa
     7681 tacttactat attatctatt ctaataattc ttcttctagc tggaactaat ataaaaatca
     7741 tctctgaaag attaggacat actaatataa aaataactat gaatagatat tttcttgttt
     7801 taaacgatat ggataaaaaa gcttctgaaa atataatcaa agttctcttt aaataacatt
     7861 ccattttatg taagtaggca ttatattttt cttaaatgtc acttaaatgt cagttatata
     7921 taataatagt aaacaactgt atttcactaa tatttgctat tatctaatag tatttatagt
     7981 ttagatttga tataatacat gtggtaaaat tttgatttgt aaaacaaaat tttacctaca
     8041 tatattaaaa caaatttaaa actaaggaga cctatatgtt aaaagtatta aaaggaaata
     8101 taatttttac aaaaacttca gaaactttca cagtatttga aaatagttat attatactgg
     8161 aaaaaggtaa agtaaaggga atatttaaag agatacctaa ggaatacaaa aacctaaaag
     8221 tagtagatta cactgataag cttataatac ctggtatgaa tgacctccat gcccatgcct
     8281 ctcaatttaa aaatcttgga atggctatgg ataaagagct tttgccatgg cttgaaactt
     8341 atacatttcc agaagaatca aattataaag atatagaata tgcaacaaaa atgtataaaa
     8401 aatttgtaaa agaactttgg aaatctggaa ctacaagaat agcagtgttt gcaacagtac
     8461 ataaagaagc ctctatgaaa cttatggata tattcaaaga agttggtctt ggagcatacg
     8521 ttggtaaagt aaatatgaat atgaattgcc ctgatacttt attagaaaac actcaggatt
     8581 ctattactga tacagaagaa attataaata agtatagtga tacagattct attgtaaaac
     8641 caataataac acctagattt ataccaagct gtacaagtga acttttaaaa ggtttaggag
     8701 atttatgttt aaaatataat attcctattc aatcacactt atctgaaaat tatgatgaga
     8761 ttgaatgggt tagaagtcta gaacctgaat ctaaattcta tggagatgca tataatagat
     8821 ataatttatt tggtcaaact cctactttaa tggctcattg tgttcattgt tctcaggatg
     8881 aaatagattt aatgagagaa aataatgtta tggcagttca ctgtccagct tctaacttta
     8941 atgttggtag tggtgctatg cctatcagaa aattaatcga taataatatt agattagcac
     9001 ttggatcaga tataagcggt ggacatacat tgtcaatatt taaagcaatg gtatctgcaa
     9061 tacaattatc taaattatat tgggttaatt ctggtaagaa gtataatttc ttatctttat
     9121 ctgaggcttt ttatattgca accaaaagtg gtggaagttt ctttggaaaa gttggtagct
     9181 ttgaagaagg ttatgatttt gatgcattga ttattgatga ttcaaattta aatcatgaca
     9241 attattctat acttgaaaga cttgaaagat ttatctatgt tggtgatgat agaaatatca
     9301 ttcatagata tgtatgtgga aaactaattg aagaacctaa tatataatta taataaaaat
     9361 tttaattata tattatggtt aatttataca actataatta ataaaagagt atattttaaa
     9421 tctaaaagct ataggttgaa acatactctt tttattttgt ttatttatag ctttagttat
     9481 taatcatata taaatctaca aaacgtattc ctagtaactt atattttttg atatctacaa
     9541 tcataagact atattattgg attcatacct caaaaatatt gccttctgaa cattattata
     9601 ttcaaagcta tgttaattta caatgatata tcctaacatg tattgattaa aaaaaattaa
     9661 aacttgcttt tcatctccta tatctaaaga agtggtattt agaataaact attgtaatat
     9721 taaagttgaa attttctagt ttatttactc tatagcacca tattttaata ctattaagta
     9781 aaaatgtagt taaaatatat actaatatcc ctgaaataag tatttctttt ttatttagca
     9841 cacttattcg taaggtatta gttattatat ttccaaatta tagaataaat attatactta
     9901 ttgctaatta taatattaat actgttaaga tatttttctt catagttata cccattattt
     9961 aatacaattt attataaatt caatttttaa acttttctta tctccttatc agaataaaaa
    10021 tcccctttac ttccttttat ttttactatt tttaaacttg ccatcttctc taatctccaa
    10081 agtataaacc aatcttttac acgcatttca tcttcaaaca tacagtttgc aaccacttca
    10141 attaaaggtt gccatttgtc actgatattt cttaatatat atttatcaaa ataagtttct
    10201 tcaacacttt taatttcgtt acctactaat gctctaaaaa tagaattttc tttaactaac
    10261 acatcccatt tatttttaaa ttctagtttt tcattaaagc caatttctag tgtcttatct
    10321 aaaaagaatg gaaaatcttc tggagacatt tcactggtaa atgaatatct aattagagca
    10381 tcttctgtct ttttttcatc tactaatgca atctttatat ttttatcttg aaacttactc
    10441 actacataat atactccaca caaatctttt gggttatttg cacaccatat tattatattt
    10501 ctacattttg ataatttcct atcttctaac aaatctggct tgaataatag tttcggatta
    10561 ttttcaaaaa catttctaat cttaaaaata ttttctctta attcgtagtt atttacatca
    10621 aaaatattac ctattgataa atcatcttca aataatacaa ttttattatg tggtattaac
    10681 tttaacttca ttgcttcttt taataacaaa taacaaatat cctcaaagca tatatgtgcc
    10741 ataaaattca cctcttttaa aattaacaat ttattttatt ataatatata aactgtaatt
    10801 ctgaacctcc catgactaaa gtcacagggt tcctgcttca cagaattttg catactaaaa
    10861 tatgtcttac taattctcca caggctatcc ccgtagttcc tacg
//
LOCUS       DAMJWU010000112        10808 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMJWU010000112 DAMJWU010000000
VERSION     DAMJWU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.116"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(153..485)
                     /locus_tag="PC460_003133"
     CDS             complement(153..485)
                     /locus_tag="PC460_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF2601996.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(729..1370)
                     /locus_tag="PC460_003134"
     CDS             complement(729..1370)
                     /locus_tag="PC460_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF2601997.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFVYSYLMYKISGKNN"
     gene            complement(1351..1644)
                     /locus_tag="PC460_003135"
     CDS             complement(1351..1644)
                     /locus_tag="PC460_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2601998.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2037..2087
                     /locus_tag="PC460_003136"
     CDS             2037..2087
                     /locus_tag="PC460_003136"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2601999.1"
                     /translation="MTEYIYYYNNKRIKKN"
     gene            complement(2613..3365)
                     /locus_tag="PC460_003137"
     CDS             complement(2613..3365)
                     /locus_tag="PC460_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2602000.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VAMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3424..5078)
                     /locus_tag="PC460_003138"
                     /pseudo
     CDS             complement(3424..5078)
                     /locus_tag="PC460_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(6350..7429)
                     /locus_tag="PC460_003139"
     CDS             complement(6350..7429)
                     /locus_tag="PC460_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2602001.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(7513..8169)
                     /locus_tag="PC460_003140"
     CDS             complement(7513..8169)
                     /locus_tag="PC460_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2602002.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            8491..8697
                     /locus_tag="PC460_003141"
     CDS             8491..8697
                     /locus_tag="PC460_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602003.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(8850..9278)
                     /locus_tag="PC460_003142"
     CDS             complement(8850..9278)
                     /locus_tag="PC460_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2602004.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(9754..10692)
                     /locus_tag="PC460_003143"
     CDS             complement(9754..10692)
                     /locus_tag="PC460_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2602005.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         3593 a         1919 c         1135 g         4161 t
ORIGIN      
        1 aaataattta aaactactat attatttaaa aatttatatt tatatatgca attctaaaaa
       61 cttaataaga agtgaactta gaaaataaag cttaatttca aaggcttaaa tagactatta
      121 ttattagtct aaataaacta cgtgtatcaa aattatatat taaattcttc taaaattttt
      181 attgcaaatc ttgcgtttgg atatgtaata gaccctccac caattacacc tatttttata
      241 gcttctataa tttcatcttt acttgctcca gaattaatac aaccctcaat atgataacaa
      301 atacactcat cacatcggct taaaattgaa atagctagtc ccattaattc cttatttttt
      361 ttacttatag ccccttctga ataagtctta tcatcaaggc ttccaaatac tctaaaaaac
      421 tcatctgttt cacttaattt tttattgtaa aatcccctat attctttcat ttctctagta
      481 ttcatcttct acctcctatt tttttcttat aagagtatca cactgtactt atattttcaa
      541 tattaaatac aaattttcat tttatgttca ttagcctaat ttatattatt ttttgaaatc
      601 tcaataagtt atcatataag taagattttt tcagaactaa aatagaaatc tcagtaattt
      661 ctttattatc tatctcagtt ctgaaatata ttattcttat ccaaacatta ttatagtgat
      721 taattatatt agttattttt acctgaaatt ttatacatca aataagaata tacaaatggt
      781 agtattacaa ttacaaagat tacaaagata ttataaaaag gaatattaat aaatgcatta
      841 ataattaata atattccacc aataagccaa atccatccag ctaaatggtg tgtcttattc
      901 caattatctt catcattaag agtccatggt aaaattattc ctactgagta atttcgttta
      961 catttaggaa gatagtttcc aaataaaata actattattc ctaaaattaa tcctgtataa
     1021 aaattagact ccaaactaac tcctgttgaa tataaaacca tcattatttg tataattact
     1081 gaaagtatag caactcccca ttttgataaa ttttgtataa catttgaaaa attagctttc
     1141 ttaggctcat tctcaagtct aaaccatgta tacaaatgga tacatagcat taacaatggt
     1201 acaccaaaag ctgctataac ctttggagca taattatctg gatttccata attatcaaaa
     1261 tgaattgcaa cttcatttgg taatctatta taaaaatata ttgatacaat aaatggtata
     1321 aaacatatta aagtagataa aataaattgt ttatttatcg cttttttcat tatctttttc
     1381 tcccttctcc ataaattgcg aaaaccaaag cataacctct tcgaaaactg aaatatttat
     1441 ttcgtagaaa ataaagttct tatactttgt ttcaaatatg agtcctgcct tttttagttg
     1501 agatagatga taagaaattg tagcattcgt catattaaat ttttcactaa tttctcctgc
     1561 tgacatttta ttctccctca acataaccaa tatttctctt cttatagggt ctgaaagagc
     1621 tttgaatgtt tctggaaacc ccaacaccat cacctcgcta tttagaattt catctaaata
     1681 gaatataaca taacatccat tttatttcaa taactattta gaattttttc taaatagtta
     1741 tttctaaata tttattgaaa attttcatat taagattctc aatttactta agtcgctttt
     1801 atttaatctt actttatcta atttttttaa cttaacaaaa gtgtattgca ccataattgt
     1861 taaagcaaaa acctaatgac tgaggtactt atatatataa caatactttt aattttaaat
     1921 taaaagtagc taaacccaat ttaaaaagta gttaagtcct atttaaatag aaaagaatga
     1981 aacgagaaat atactgtata aaagtacata aaaacatcta ctaattagat acaactataa
     2041 cagaatatat ttattattat aataacaaaa gaataaaaaa aaattaattt agcatagcct
     2101 tgttaaatat agatttgaat atgaatttaa agttatgtaa aattaaaaat acaggaatga
     2161 aaattccatt cctgtataaa atctaacttt tgagtctcag tacaatctta ttttgagaca
     2221 atcttttttg ttttatattt tcttatattt aaaatataac gcatatctta tgcaacatat
     2281 tcatattttt ctttatattc tttcatcgca tcattccaag cgtcttgtac tgttacacct
     2341 cttttattag caattttttg tgccagttca attatcttca tagctcttct tgatatgttc
     2401 ataaacattc ctcctacaaa ttaccatata tttcatatat ataaatcaag gttatatttt
     2461 tatgatatat aatccttcta ttgtaagatt acagtttata tgtgtattat atatgaacta
     2521 tattagaaga taattagaat ttttataaag ctatatgatt agattaaata taaaagcttt
     2581 acaattactt ataaatttaa aaagtctata aactaaacta atgtttgata taccaatttt
     2641 acgaccataa ataatgccat tactaaaaat attggtttta tcatctttga acctttcttt
     2701 atagcaactt tagcacctac ataagctccc attatcatca ctaatgcaaa cactatagca
     2761 attttataat ctacttgacc atttatcatg aataataaca atgaagttat attacttgtt
     2821 aagtttaata tttttgtatt tgcagaagca tgtaaaaaat catatccata tatttttata
     2881 aatccaaaag tcaaaaaagt tcctgtacct ggaccaaaaa aaccatcata aaaacccatt
     2941 ataagagcca taagcatccc tagtcttaaa tttttcttat taacagcttc aaaattatct
     3001 tcttgaccta aatttttaga aataaaggtg taaatagcca ctattaaaat catagccaca
     3061 accaaaacat ttaatatctc ttcacttatt gataatacac atctcacacc tagaacacta
     3121 ccaataatag taaatggtac taattttttt aaaaggttgt tattaatttt tccagacttt
     3181 gcatatctat aggcactact tatacatcct gctgatgctg caaatttatt tgtaccaatg
     3241 gcaagatgta ctggcactcc aattgccata agaacaggca tgcttataag tcctcctcct
     3301 cctgctatag aatctacaaa agcagctaaa aaccccccta tacataaaaa aataactgaa
     3361 aacataaaat tcctccatca ttctctttca atttttctta caatatatat tatataatct
     3421 tatttaatac ttattatcca gcatattctt tctatattca tttggagtta tactattaaa
     3481 cttcttaaat atttgtgaaa aataactact gttattaaaa cctactttct tacaaatatc
     3541 actaacactc atgcttctat ctaacagata ttttttagat tcttctatcc taagaagatt
     3601 tatatactca gttattgaaa ctttatttct ttcattaaaa atagaactca aataatttgg
     3661 acttataaaa aaacatctga tatactattc aaatttatat tttctttata atattttttt
     3721 atatattcgt ttatattttc aattagattt cctttatctt ttctccaaaa tgctctattc
     3781 ttatcaatac actctttgga aaattcaaat aatacattcc ataaatcttc atcactgttt
     3841 acaagtgata tttttttatt atatttattt cttaaataag atataagttt taaatcctct
     3901 aaatctctaa gcactgttct agacaataca aatatcattt cctgaatagt atctctaaca
     3961 tcattatatc ttaatttgtt tgtatctatt agattataaa caccttctaa attcatattt
     4021 ttaatttctt tttcaatata aagttctaat tcaaagggat attcttttga agaattattg
     4081 aacatatcta tcggattaat aatatactct tcttctaata atctactatt tgctttttta
     4141 agtgcctctt caagtcttat attttctttt ctactcttca atatctcctc atcttttata
     4201 ctaagttgat tttcaaaaaa attcctctta gctatttcct gaataaattc agacatcatt
     4261 aataaatttt cttttaatac atataatcta cttttaggat ttaatggaaa ttcattgtaa
     4321 attttgttta tgtattttat atcaatacca gaactacttg ataattcaga taaattttta
     4381 tctatataaa tagacttata tactataaaa ttaccacatt ctatatatcc aactatattt
     4441 tcttctaata ctataggtat ctctaaactg gaaacaccat actcacaatg atacactttt
     4501 tcaaagttat cattatttat gcttttaaca agttttttag tacacggttt tatattttta
     4561 caaatatcgc aaaatttatt ttctttctta gtctcaaata tttctttata ctttaagtca
     4621 tatacagata ttctaagctt caatatatta gatatttgat ttgcaagatt ttcaattatg
     4681 tttacattaa ctaactcctg tatattgttt tcagaaaata ctataggtat ttgtctcaaa
     4741 ctcgaaaatt tatgaggaac ataagtaatt aatattacta aatcaatttt accagtgttt
     4801 ataatttcat gttctgaata tggtgggcaa taaactatag atgtttcctt tatattgacc
     4861 tttttaccat caattatttg tattccttct ccctgaatca catataaaag ctgttcttca
     4921 cttaaatgaa aatgcttttc ttggcagttt cccggaaaaa atcttacaat tccagcacta
     4981 agtctattga attgtgtttt tgatggttcg tgtatccaca taacctctcc ccaatcaaat
     5041 ttttgtactt cattatttga ctcaataaaa tccaccataa actcacctcg tagtttttgt
     5101 ctaaatattt tttcttccct tcttatatta aaataatatc atcatcatta actttcatta
     5161 ctataaaatc atcatactct tttaatactc ctgtaatttt agttgcactt agtttactac
     5221 aattcagttt tttaatatat tgataaattc tataattact tttttatttt gtaaaatcag
     5281 tgttttccca aaataattat atctataaat gaaagtattt ttactgctta catcaatata
     5341 tgttatcata attttccttt ttattgcata tataaataac tgtatataac attgtccaat
     5401 actatataga aatacatcta agaaagaata ttattaaatt gttccaatta atattatttt
     5461 tcttatagaa ataaaaaact gttggattct tttagaaaaa ttatttttta catacctttt
     5521 ttgcttaaat agtctatctt ctcagctatt ttgtatatac actcttctag ttcatgattt
     5581 tattttaata tcttgcttat attgctgaaa taattaaatt cttttatcta tcatattgtc
     5641 tcttatatca caaacatatt gtttcttata tcacaaaaat gaaagcattc ttatatcttc
     5701 aatatataaa cctgcttttt ttaatcttgt aataaatata ggatactcta tattctcttc
     5761 ctagaaataa ctaataccac ttatttttct atcatataga aagtaataaa tctttttctt
     5821 aataaaattc aatagtaagt ggaataaaat tttatatgaa taagaacatt ataaaaatac
     5881 tttatacaat agtcaataaa agatattagc ttcaaattgt gttgaagttg tattatttaa
     5941 agtggaaaag caaataaaaa agaaataatg gtataagtag aaaaatatac ttaaaacaag
     6001 gcgaagcctc tcttgtatct aaatcaagta gagaattaaa tctaaaaaag aaaataaata
     6061 taaaagatga ctcctgtaca ttacaagaat catcttatgg tttcataatc actccatggt
     6121 ttttaactgt ctgatataaa ggtctcagtt cactcatagc atgaccacct tttccgattg
     6181 ctgaccccgt cacttatgga caaaatgtcc ataagtgata gtcctctctt aaaaccctct
     6241 aaatagatat atctcaagca cctcattgaa accagttgct ggaaacaaac tgaagcgttc
     6301 tggaatagta aaagaaattg ccaccatatc cacgttactg tcctagttgt tactttgcta
     6361 acttcggagc gatttcctct aacaatacgc agtcaaattc tgcctttttg ctaagaaatg
     6421 gcagatggtc tgcatctggt ataaaaaaaa tctgtttgtc tggcgccact atttcatcaa
     6481 aaaatttctt cgctggaaga taggggctat tatagtcatt ttctcccatg atgtagtata
     6541 cagggacctg atagtcaact ccataatttc ttatgtcagc ttcttccact aaaaaccgca
     6601 atacatcacc ttgattgtca atcgcatttg gattgacaat ggccgtattt tttaactctc
     6661 tcaatgaata atatggggtt gccagtatac tgattatatt gcccaaatcc atatttatag
     6721 aaagcccata cttgctttgg tatgttctta ctgtcataag agctgccaaa tagcttggac
     6781 catattttgc tggcggatat ggtgcaatag cttccagcgc cttgatatct ttttgcttcc
     6841 ctgcagcttt caccatttca agtgtctttt catagcccac gcgctcattg tccaccatat
     6901 taacagtctg cgcaatccca atgtacgcgc tgtaatcctc cggataggtt tgcacagcca
     6961 tagttcccag cactgttccc cagctatgac ctataaggat aattttctcc ttgttgaact
     7021 cttgcttcaa atatgctgtc acctcatgga cgtcttgcag aacacgctcc gcgctcatgg
     7081 tctgcaaaac tgctgccgga tcgttcgcaa agtatgtttt gcctacgttt cgctgatccc
     7141 agttcaccac agtaaaatca ttttcccact catactggta cagatgtaaa aatggcatca
     7201 ttgaattacc aggaccccca tgaacccaaa ggatgatggg gttgtctgcg ttttgtcccc
     7261 ggatatataa cgcctgctgt atgccgccga tctccaccat ttccatgcgg tcgattccag
     7321 gagcttctat tttatggctt gtacgtataa tcgtgggggc tatcagacgc aaaccggcaa
     7381 acgccagaac aaaaacagca actagtatgc aaagtgtgat taatatcatt ttaagtacct
     7441 tctttcatga atttttttag ccatagatat ctcctctaac attaaaaaaa gtatgttgct
     7501 attttagttt tgttactcta cctgtcccat tggtttcctt tcataaaaaa tattaattga
     7561 ttctataatc tcatcttcca ggatatcaac tcctatttgt ctcataagaa actgtaagaa
     7621 ttgaaaccgt ttacgaagtg taggaaagtc cccctgaaat acatctgtat cacaccaaaa
     7681 gttaaagagc agcaaaatta cctctgcgca ttcatctggg aactctgtga tgatgcttct
     7741 atcctctatg ccttctcgta aaacatttgc taatattggt gctagttttt tcaaattatc
     7801 ctgtgtaaat gccagtatga tatgcggact ttctatcgcg cttgtaatca tgttactaga
     7861 ttcttttatc acctcatccg ttaaatgccg cctaactaaa cttctaagct tctcttttgc
     7921 tgtaagtcca tgcatttcac cgagccattg atttactgtc tctattatct gttcaaagcg
     7981 cctttccatg accacattaa aaatatcttc ttttgaactg aagtgatgaa aaattccgcc
     8041 ctttgacatt cccgaagcat caacaatatt ttgcatactt gttttgtcgt atcctttttc
     8101 agcaaacaac tttgcagaaa tggtaatgat attttcaata gcttgctttg gattgttctt
     8161 tctagtcatt atataactcc ttcttattaa aaaccgactg ttggttttta ttatataagc
     8221 tattcctgct tctgtcaagt aacattgtgt ttatggcaaa acaataaggt ttggtggtta
     8281 gacaggtatt aattagacta atgcgacttt cccctagatg gaacccttgg taaatttgca
     8341 atctttaccg gctttgatta tatctgtaag caaggcaggt atggccacgc ctgccctgtt
     8401 tgaccctcgg atattcattc ctatccctcc tctccttagc cattacacca aaacgccgct
     8461 cattgtctcc aatggcggcg ttttaaagat atgatttctc atgctgctga aatatgcaaa
     8521 aaatataaga tgccctttat tatttttcct acaatagaaa agaaaagtat tgaagaatgg
     8581 gttaatattg gagcaaatat gctttgtttt gcacaagata ctacctttat cacttatgtt
     8641 atcaatcaat tattatcttt ttataatgag attgtaccaa ttcaaaaaca acattaaatt
     8701 ataaatctaa ttttatatta aaagagacta atattttatc tatctctatt aaggatattt
     8761 tcttatgtat cataatatta gtctctctta ttattatata ctgctatcat cattagttgt
     8821 tatttatact atttttataa tatacaaatt tattcagcta acgttttgcc taattctata
     8881 cactcatctt ttgcttcttc atttgggtct tccattgctg taattccttc tccaccaata
     8941 attgtagcac cagcattttg catacgttct tcccagtctc tcatccattc acaatttccc
     9001 catccatatg aaccaaataa tccaatctgt ttacctgata ccatagattc taattctgaa
     9061 acaaatggct ccatataccc ctcttctagt tgctctgctc ccatagatgg gcaacctaat
     9121 gcaaatttat cttcttcttt taaatcactt ggtttcagta agctcacatc taaaacttct
     9181 ggtgtcttac cttttaactt cacaccttca gccacaaaat ttgccatttt ttcagtattt
     9241 cctgttccac tccaataaac aatatatatt ttactcattt ttacctcctg tagtattttt
     9301 taattaatca atagttagtt atagctaact aacatctaaa aaacaaaaga tttctttatc
     9361 tgaaatctct ttatttttct aaatatagaa tatcatagtt gatattgaaa ttcaatactt
     9421 attgataata tttatcattt atattttata ttcattttat aatatcaaaa cactgtatcg
     9481 tatttctaat ctttcataga aaaatttcta acttatagta cttttgcatc tcttataaat
     9541 cttttatatg tataagtaca tagaaaaaca caccttttcc agatgtgcta tctataatat
     9601 cgtatattta tttttcaatt ctaactagct aatatgttta taaattttat cacttaatac
     9661 ctttatacat tgatgtaaaa tcttacacct aagtaatttt tattatttgt ctcgactcta
     9721 tataatatag ctattttatc atcaaatcaa aaattatctt ttactattat tcttgtattt
     9781 atagagattt aaatataagt atccatttcc ttgttccgtt ctaggtattc caagcgctct
     9841 cgtacatttt attaattgtg catacagttc agcttcatct aaatctcttt caaactcctc
     9901 tcttgcccat tctttaatta gtgctaatga gcctgatacg tatggtgcac tcatactagt
     9961 accactcata atagcaagtt tattatccat ataagttgat ataatatttc ttcctggagc
    10021 taccaaatct attgtagtat ttgaattact aaacttttca accaaatagt tctcatttat
    10081 tgcacctact tctattacct cagcataact ggctggataa ctatactcac ttgtactaga
    10141 atcaccatct ccattattac ctgctgcaca cactacagaa atattatttt ttactgcttg
    10201 catgacagca ttttttaaat tcttatcatc tttgtttccc ccaagagaca tagatataat
    10261 atcaaccttg ttatttacag caaagttaat agcattaatt atactttgat atgttccggt
    10321 accatcttta tttaatgctt ttgctattaa taatttacaa tctggagcta ctcccataac
    10381 ttcattatta taattagatg cagctataat acccgccaca tgagttccat gaccattaaa
    10441 atcctcatat atatttttat ttccattact gtcatcacta aaatttgcac caccaattat
    10501 ttttcctttt aaaagaggat gagatatatc acaacctgta tctattatac caactacaat
    10561 atttttacca gtataacctt catcccacat tcccctagca ttcatttgtt ttattccata
    10621 tgggaattta ttttttgcac ctcttagatt ttcatttatt tcatatggaa ttagtttcat
    10681 tcttttattc atcatatatc ctcctaaaaa ttttattgct tataaaaatc atgaatttta
    10741 ttttataata ggtttataaa tcaaattaaa acctatatct acttatttat atttatgata
    10801 tatttttt
//
LOCUS       DAMJWU010000113        10714 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMJWU010000113 DAMJWU010000000
VERSION     DAMJWU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.117"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            63..911
                     /locus_tag="PC460_003144"
     CDS             63..911
                     /locus_tag="PC460_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF2602006.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            975..2897
                     /locus_tag="PC460_003145"
     CDS             975..2897
                     /locus_tag="PC460_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2602007.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            2979..3842
                     /gene="aroE"
                     /locus_tag="PC460_003146"
     CDS             2979..3842
                     /gene="aroE"
                     /locus_tag="PC460_003146"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2602008.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4042..5259
                     /locus_tag="PC460_003147"
     CDS             4042..5259
                     /locus_tag="PC460_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2602009.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5495..7360
                     /locus_tag="PC460_003148"
     CDS             5495..7360
                     /locus_tag="PC460_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2602010.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7538..8902)
                     /locus_tag="PC460_003149"
     CDS             complement(7538..8902)
                     /locus_tag="PC460_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2602011.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9081..10424)
                     /locus_tag="PC460_003150"
     CDS             complement(9081..10424)
                     /locus_tag="PC460_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2602012.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10497..10598)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         3959 a         1358 c         1837 g         3560 t
ORIGIN      
        1 taaaaatata aaatcaaaaa tactattaat tataaagaaa ataaaggggg caaatttaag
       61 ctatgaataa tcaaaaaaat cttaatattc caattacaat tagttcttat acattgggaa
      121 cagaagtttc attccgtgat agagttcgta ttgcaaaaga agctgggttt gatggtattg
      181 gattgcgtgc tgaaaattat attgatgcaa aaaatgcagg tgttacagat gaagaaatga
      241 ttgcaattct taatgaatat gatatgaaag ttacagaagt agaatatata actcaatggg
      301 gaagagaaga agatagaacg gaagctcaaa gagaaaaaga gcaaaatgtt tatcatatgg
      361 ctcacttatt taatgttaaa catataaatt gtggattatt agagatgata ccagaagaac
      421 aaattataac tgcattggga gaattatgtg acagagcagg tgaacttatt ataggtcttg
      481 agtttatgcc atatagtggt gtaccagatt tggctactgc atggcgtgtt gtgaaaggat
      541 gtaatcgtga aaatgcaatg cttatattag atacttggca ctgggctaga gcaaagcaga
      601 cagcagagga ccttgcacct gttccagcag ataagatagt atcaattcaa gtgtgtgatg
      661 ttttagaaac tccttatgaa aaacttaggg atgaatcact acatgaccgt ttggctccag
      721 gtgaaggata tggaaacaca gaggaatttg tgaaaataat aaaagaacac aatatatcac
      781 caagagtcat aggggcagaa gtaatatctg attctctaat atctaaaggg ttatccactg
      841 ctgctgaaac tgtatttaat gcaactaaaa aagttttgga taaagcatgg tctgaagtat
      901 ctccaaaata aacttatata atataaaaat atacagagta ttataatata aaacttgaaa
      961 ggaaggaaaa ccttatgaag attagttcta tgtttacacc tataagaatt ggttcaatga
     1021 cagttccaaa tcgttttgta gttcccccta tgggaaacaa ttttgcaaac acagatggta
     1081 ctttaagtga aacttctaag gcttattatc ttgagcgtgc tttaggtggt tttggattaa
     1141 taactataga atctagtgtt gtagacaaaa aagcaaaagg aggtcctcgt aagccttgtt
     1201 tatatgatga tagcacaata gatagtttta aaaatgtaat agatgcttgt catgatgcag
     1261 gttcgaaggt atctatacaa ttacaacatg ctggttctga aggaaatgaa aaagtagcag
     1321 gacatccatt aaaagctgct tctgcaatac ctgcatcaaa tggtcgtaat acaccactag
     1381 caataacaac agaagaaata tatgaactta tagaatctta tggagatgct gcattacgtg
     1441 cacaaaaagc tggtgcagat gctgttgaag ttcactgcgc tcatggatat ctggttagta
     1501 gttttatatc acaaagaaca aataagcgtg tagatgagtt tggtggatgt tttgaaaata
     1561 gaatgagatt acctcgtctt atcattgaaa atatacgtaa aaaagtagga aattctctag
     1621 caatattatg tcgtataaac agcactgatg atgttccagg aggaattgat gtacacgata
     1681 gttcagtaat agcagcatat ctagaagatt gtggaataga tggacttcat gtttcacgta
     1741 gtatacatat acatgatgaa tatatgtggg ctcctacgac tttacatgct ggattcagtg
     1801 cagaattagt tactgaaata aaaaaagctg ttagtatacc agtaataaca gttggacgtt
     1861 atacagagcc acagtttgct gagttaatgg tcagacaagg aagatgtgat ttagtagcat
     1921 tcggtagaca aagcttggct gacccagaaa tgcctaataa agctaaaaat ggaaaactag
     1981 acgaaatgat accatgtatt gcatgcttac aaggatgtgt accaaatatg ttccaaggca
     2041 aacctatagc ctgtcttgca aatcctatat taggacatga agcagagctt aagccagctg
     2101 aaattagcaa agaagttctt gttgttggtg gaggtgttgg aggtatgctt gcagcatggg
     2161 tatgtgctaa aagaggacat aatgtaactt tagttgaaaa atctgaagtt cttggtgggc
     2221 aaatgcgttt agctgcatat cctccaggga aaggagatat tactaactta gttagaagtt
     2281 atatttcaaa atgtaatcaa tatggtgtaa aaatatgtac taatacagaa gctacagtag
     2341 aacttataaa ggagaaatca cctgatgtag ttattatagc tactggagca actccacttg
     2401 tacttcctat accaggaatc aatgattcag gattaattca tgctgtagac cttcttgatg
     2461 gtaaaaaatc ttgtgggaaa aaagtacttg ttgttggagg tggaatggtt ggatgtgaag
     2521 tggcagcctt ccttggagaa caagaacatg aggttactgt tattgagctt agagaagaag
     2581 ttggtgcaga tgtaatttca gaacaccgta aattccttat gaatgatttt gaaaattata
     2641 atatacatac cattactggt gcaaaagttt ctaaatttta tgatgatggt gtcgactatt
     2701 tacttacaga tggcacagag catgatttaa aaggttttga ttcagtagta cttgctatgg
     2761 gttctcgtaa ttacgaccca ttaagtgaag ttataaaaga agttgtaaaa gaaacttata
     2821 ttgttggtga tgcagtaaaa gctcgtaggg cattagatgc aacaaaagaa gcttttgaag
     2881 tagcattaaa tatataaata ataaataaat ttttctttgt aaataactga ataggttatt
     2941 tgcaaagaaa atgataatat tgagaggaga ttatagttat ggaaatttca ggtagaacag
     3001 gattatttgc attaataggt acaccggttg gtcactctaa atcaccagtg atgtataatt
     3061 atagttttaa gaaattagat ttagattata gatatttagc ctttgatatc acagtagata
     3121 aggtaaaaga ggcactttta gcaataaaaa cttttaatat aaaaggagcc aatgttacta
     3181 tgccatgtaa atctgctgtt acagaatata tggatgaact ttctccagca gctcgtataa
     3241 ttggagcatg taatacaatt gtaaatgaca atggaaagtt agttggtcat ataacagatg
     3301 gagtaggcta tgttcgtaat ttaaaagaaa atggggtaga agttaaaggt aaaaagatta
     3361 caattatggg tgctggtggt gcagcaactg ctatacaagt acaatgtgct ttagatggag
     3421 caagagaaat atctatcttt aatcctaagg atgattttta taaaagagca gagcaaacag
     3481 ttgagaatat aaaaaaagat gtaccagaat gtgttgttaa tctatatgat ttagaagata
     3541 caaacaaact ttatgaggag atacaatcaa gtgatatatt gacaaatgca actcttattg
     3601 gtatgaagcc ttatgacaat gaaacaaata taaaagatac tagtgtttta agaaaagatt
     3661 tagtagtaac agatgtcgtt tataatccta agaaaacaaa aatgattgaa gatgctgaag
     3721 ctaatggatg taaggctatt ggtggattag gaatgttatt ataccaagga gcagaagctt
     3781 ttaatctata tactggatta gaaatgcctg tagaagaagt taatgaatta tgctttaaat
     3841 agataagagt aatatcttaa acaatgaaaa aacatacgta tttcaatatt tttaataata
     3901 aattacaaat tgtcatagtg ggttttgcct catagtccag ttaaatttga agttttaact
     3961 gactatactt taaatccatt atggggatga gtttattgta cacaaaaatg ttaataaatt
     4021 aacaagaatg ggagaaaaat tatgaacaat aaaaaatatt atcctactgc catagcgttg
     4081 tatttttcat attttttact tggtattgga atttcaattt taggacaata caaaccagaa
     4141 ttttcatcaa tgtggggggc taaaacatta tcagatggaa cattggatgt aagtattgtt
     4201 ttagctgtta ttgctgcact aggattaggg agacttatat cttatccatt tgcaggacct
     4261 atttcagata agtatggaag aaaagtaagt ggtcttatag gaaactttct acatgcaatt
     4321 ttctttgttg gaattgtatt ttcaccaaat ttttatatag catatgtatt tgcaataatt
     4381 ggaggggcag caaattcatt cttagatacc tgtgtaactc cttcttgtat ggagatattt
     4441 gctagtttag gaacaatagc aaatatgttt actaaattta caatagcttt agcacaattt
     4501 ttgcttcctt ttataattgg atttgttgca gcaaattcaa tatcatttaa agttattttt
     4561 atagtaacag cgattcttat tgtagtagat gcctttttga tagctgtgtt accatttcca
     4621 ccagctaata atgttattga taacaaaggc aaaacagtta aatcagagaa gatgaaattt
     4681 acaccaacaa gtatagctct tgtttgtatt ggatttacat gtacatctac atttgtatta
     4741 tggttaaatt gtaatcagga attgggtaaa ttgtatggga tggctgaccc tacaaaaatt
     4801 caatcctttt actcaatggg agtaatatgt gcagtattaa taacttcttt acttattaaa
     4861 aaatatatta aaccaataag aatattagtt atatatccta taatagcttt acttatgcta
     4921 ttagttgtat attttgttca aactccaact atttgtatgt tagggggatt tgtaataggt
     4981 tattcagcag caggaggtgt tcttcagtta acaacgtcaa ctgcaaatga aatgtttcca
     5041 actaataaag gaaaaataac atctatagtt atgattgcat ctagtatagc aaactatgtt
     5101 attttaaata tagcaggtat tattacaaaa tcagggggag ttaatggacc aaaatatgtt
     5161 gtattgttta atgtggcaat aacatttgtt ggaattttac ttgcattgtt tgtaaacatg
     5221 agatatgaaa aagaaaaagt gtatgattat gatgtctaat attagtataa tatatgcata
     5281 gagaagtcta aatctaggat agatttttat taaaagtaat taaaattaat aaggatatta
     5341 tttacagtta taagataggg tatttacata attaataata aggatattac tagaatttca
     5401 atttctagta atattttttt gcgtataaat gtaaacttga tgaaataagt agtataatca
     5461 atataaatta catcatgtaa aggaggataa tttggtggag aatgttttta agcgattgca
     5521 ggaatttaat ggttatgatg gatacaaaga atcatttgaa atgaattacc tatgtatata
     5581 tgaaagtata ccgttaagag aacaagtaga gcttgcaaat aatctagtag atgaaatttt
     5641 aaatatgtat aaaagtgaat ctaatgaaat ttatttattg gagggtagta attctaagtc
     5701 tttaatttgt tatttcgaaa tttttatgaa aaagataaat actttggtaa aagaaatgat
     5761 tattgatgag aaatggttat ataaattaac taaagaatta atatataaga gtaaaaaagt
     5821 tgagtatgta aaactagggt tagttttaag tgagaaatat ctaaatgtag aaaacttaag
     5881 agaagttgta gatacatttt ctaaatctgg agaatacgta ttttatctga gtaatactat
     5941 taaaaaactt gaattttaca atacatattt atttaattta tctaaaaagg ctacaggttc
     6001 aataaaagta tttgctattg ttaatatgga aaatttagat tctaaaataa attcctatct
     6061 aattgaagat ggttataagg atacaaagta cgaacgatta ttgatgaact atattatttc
     6121 aattgtagat ttgaatgaat atcttgaaaa acgagattta gataaagaaa agataaataa
     6181 tttagcacgt cttatatgca attacttgct aagtgtagaa tttaaatata taggtaataa
     6241 attagaatta gtgaatcgat ttttacctac tgtagtaaat tatgggacta actttgaaag
     6301 tttatattca attttcctaa tagctataaa cgtcttaaaa gatgaaaata tagagtgcaa
     6361 taagattgaa tttgaaaaag aaataaatgg tatcttgctc agtgaaaaat ggaaaaatat
     6421 atattttgaa gcattaagag atgctagtgg aaaaacagaa gatattataa aaatgtcaga
     6481 aatttatgat gtaaacttat cttttgatga cttgttgcca tatctaaata gagatattag
     6541 agattttgag gtatattggc atatatctaa aaagggaacg acaagttcta gattaaaatt
     6601 attaaatttt tttgaagaga cgtttaaaat agatgattta attggaaaaa tgaaagatat
     6661 agaaaaagat aaattgactc aagaatatta tgatgatatg ttatttttta ttgtgcttaa
     6721 aggaagtaaa tcactatatc ctgaaggaaa gaatatatct ttaaaaggaa tatttggaaa
     6781 tataaatgaa gtaagaaaag aatccataaa tatcttaaaa agatacagag agaaactatc
     6841 tcttgaagaa ttaaaaattg ttaaagaagc gtatgaaaaa gaaaaaaatg ttatattgaa
     6901 ggatgaatta agaagagtat tatatgaaag taataattta aaaaaagaat ttgtaaatat
     6961 tgaaaaaata aaagtagatg aacatggtaa ggatatctat ttgacttcaa tagctgttgc
     7021 tggttcaagg tttagaaata gagaatatct tgaaaaagaa ttggagaaaa gtaagatata
     7081 ctatttaact agagagaaag ataatctata tgatgaaaaa gctattaaaa tagttggtga
     7141 aactggatat gtgataggct atgtcccaag aaaagaaaac tatattttga gcaatttatt
     7201 agatggagga aaactgctgt attgtagagt aactgaatat aatttatatg aagattgtat
     7261 ctacgcaaat gtgtacttga gttataaaga tgtaattgaa acagttgaaa actctttaaa
     7321 gatggtttta gataagagta gaataaaact tataaactaa gacgtttata tattgcttag
     7381 atgatgtaat cctaactgat tggttatttt ttctggaatt atggtgtatt ttatgagcaa
     7441 tgctaatgaa tataaaataa aaaaacaatt gttttgtata tatgatgtat ataaatatat
     7501 ttatatattc atatttacaa aacaattgaa atttatgtta ttttataatt ttaaatttag
     7561 atggaatttt aacttctgct ttatctttta ttttagaagc ttgtttttta actttattta
     7621 ataattcaga ttcatctaca actaatactt ttctttcctt catcaaccat ttaccatcac
     7681 aaatagtaga gtctacattt tcactagtca tagaataaac tatatttcca attggattat
     7741 gcattggtaa acaatgaatt gtatctggat ttagaataat catatcagct ttttttccta
     7801 cttcaagact accaatttct ttttctagta aagcacattt tgctccattt atagtagcca
     7861 tttctaagac ttcttcagct ggtacgacag ttgggtttaa agttcttcct ttatgtatta
     7921 gtgatgtaag atacatgtcc ctcataaggt ccattctatt atttgaagga gctccatcag
     7981 taccaattga aacaggtatt cctttttcta acatttcagg aattgatgca aaccctagaa
     8041 ccactttcat agctgaacct gggtcatgag aaacttttac atcatgtaat ctaaataaat
     8101 caatttcctt atgagtaagc caaactgtat gagcagctaa aagatttggt cctaatacac
     8161 ctagtttatc aaggtattca actgttccaa ctccattgtt ttgctttaca aatgaaattt
     8221 catcagctat ttcagcaata tgcatatgta tacctgtatt gtatttatca gctagttctt
     8281 tagttctaac aagtaattct tcagttgtat taaaaatagt tcttaatgca aaccatattt
     8341 taattctctc attagatgta ttattaaact tatggaataa gtctaattga gtgttgattt
     8401 cctcatcagt agttttttgc catgcctcag gaagaccatc tcctgtatcc ataactgatt
     8461 tagctaaaca ggctctaagt ccagttttat caacagcttc aaccatagca tctacatatt
     8521 gaccaccaga ttctaaaaat gtagtgacac cagattttat caattcaaca caacatgcag
     8581 tagatgaaat caaggaatct tcataattga agctactttc gtatggccaa attctttctc
     8641 taagccaagt taaaagtaca acatcatctg caagaccacg tcctaattgt tgagataagt
     8701 gaacatgagt attgactagc cctggcatta gtatttttcc tttagcatca tatatctctg
     8761 catctttatc tacaatatta atatcaaact taccaacctc ttttataata ttattttcaa
     8821 taagtatgct tccattttca tatacctctc tatctgcatt catagtaatt agatatgcat
     8881 tttttataag taattgtgac ataaacacca ctccttttct atatattttt atatctatat
     8941 attatgataa taaattttct atacaaagtt aatagcaaaa tcaatttaca tataaagtga
     9001 ttttgctatt tataaattta aaataaataa gattttattc tatataattt aaaatattga
     9061 cctagaacta tttaaaaata ttaacttaga actaagttta caactgcaag taaacctata
     9121 atccaaagtg taatactgat tttatcagtt tctttagcta ctacatgcat tattatatat
     9181 gaaataaatc caaagcataa accaatactt atactataag ttaaaggcat caataaaact
     9241 gtaacaaaag caggtacagc tattttgcta tcagcaaaat caatctgttt tatgctttca
     9301 aacatgaata caccaaccat tacaagtgca ggagctgcag cgtaagtagg aactatacca
     9361 agtaatggtg taaatataag tgtagctaag aataatgctg ctgttactat tgaagcaagt
     9421 cctgttcttg cacctgctgc aataccagaa gcagattctc caaatgttgt aacagtacta
     9481 gttcctaaga atgagcctat taaagtagaa gaaacatctg catataacat tcttcctaaa
     9541 tcttttattt caccattctc atcttcaaga cccatttgtt tacaacaagt aataagaaat
     9601 gctaatgaat caaacatgtc tataaacatg aaagagaata taggtccaat taatgagaat
     9661 ttcaatgcac ctacaatatc taatttaaaa gcaactggtg ctatactagg aggtgtcgag
     9721 attattgctg ttggcaactc cacaaatcca aatataacag atattattgt tgcaactact
     9781 atacttatca atattccacc tttaactttt ttatattcta gtattgccat taagataaga
     9841 gcaaaaagag ctattaaaac tgttggtgta aaatctgcaa gtgaaactat agtatctgga
     9901 ttagctatta taacacccat attttttaaa ccaacaaaag caaggaaaag acctatacct
     9961 gctgaagatg ctactgccaa ttctctagga attgcgtctg caattgtctt tcgaagacct
    10021 gttactgcga gtgtaaagaa gacacatcca gatagaaaaa ctaccccaag acctgtttgc
    10081 catgggattc ctgcacctat aaccaaagag tatgtgaaga atgcatttaa tcccatacca
    10141 ggagctagag caaatggtgc atttcctaaa atcgcaaata taagtgtacc aatggctgta
    10201 gctagtatgg taactgttat aagggcggct ttatccattc cagcttcaga taatattgct
    10261 ggatttacga aaactatgta tgccattgtt aaaaatgtag taactccacc tattacctct
    10321 gttttaaggc tagagccacg ttcgctaaat ttaaacattt tgtctaaaaa gcttaatgat
    10381 gaattatttc cacctggttg tacattgtta gtgtttggta ccatttttac ctccaaaaaa
    10441 ctttaagata taaacaaaaa aatatatatt catatagata gaatagaaca tatattcaga
    10501 gttgacattc tattcataga aaacccattt aaggtgagtt tgtagaaact attagaccgt
    10561 attactaata ttatatgaac attttctatt tgtttttctg ttgtattata tcgtaatatt
    10621 gtattattta caataaatat aataaaatta ttcgtttttt tgtcgatttt ataaaaaatt
    10681 aaggttattt attgaaaaaa attacttttt ccaa
//
LOCUS       DAMJWU010000114        10366 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMJWU010000114 DAMJWU010000000
VERSION     DAMJWU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.118"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            196..1620
                     /locus_tag="PC460_003151"
     CDS             196..1620
                     /locus_tag="PC460_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF2602013.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANESEEDSTSLEEAKAKMSDMKSASKNSEKNIEKKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLDIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1655..3784
                     /locus_tag="PC460_003152"
     CDS             1655..3784
                     /locus_tag="PC460_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2602014.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFSLASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIIK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKGKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNTKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIMSIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDE"
     gene            3814..4236
                     /locus_tag="PC460_003153"
     CDS             3814..4236
                     /locus_tag="PC460_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2602015.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAK"
     gene            4255..5406
                     /locus_tag="PC460_003154"
     CDS             4255..5406
                     /locus_tag="PC460_003154"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2602016.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEIYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSNGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5512..5613
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5796..6368
                     /locus_tag="PC460_003155"
     CDS             5796..6368
                     /locus_tag="PC460_003155"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF2602017.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKEEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6432..7082
                     /gene="fsa"
                     /locus_tag="PC460_003156"
     CDS             6432..7082
                     /gene="fsa"
                     /locus_tag="PC460_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2602018.1"
                     /translation="MIFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHEGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7234..10158
                     /locus_tag="PC460_003157"
     CDS             7234..10158
                     /locus_tag="PC460_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2602019.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKEPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPVLNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4252 a         1009 c         1950 g         3155 t
ORIGIN      
        1 ttatttacaa aatcatatac tccaaaatac tacataaata aaattaaaca aaaactaaaa
       61 tgacaaatgt cattatctaa agtgacatag aaacggtttc ttaaagttgt tactataaaa
      121 tataaactct ataataaaag tataacaaaa gggaatcata tcgatataga atataattca
      181 agaggaggta aaattatgag tagttctaat caatcgacaa taaagacatc tatacagcga
      241 gtagggaaat ttttaagtgg tatggtaatg ccaaatatag gagcttttat agcatggggt
      301 cttataacag cattatttat accaacagga tggatgccta atgaaaaatt gagtactata
      361 ggcgacccaa tgataaaata ccttttacca ctattgatag cttatactgg tggtaaagca
      421 atagcaggac aaagaggtgg cgtaataggt gcagcagcag ctatgggagt aattgttggg
      481 gcagatatac caatgtttat cggagcaatg ataatgggac catttgcagg atgggtaatt
      541 aagaagttcg ataaatttgt agatggaaag ataccaacag gatttgaaat gttagtaaat
      601 aacttttcaa taggtataat aggtatgctt cttgcaatac taggatttta tgcaattgga
      661 ccagcaatag tagcagggac agctcttata gaatctggag ttcaatttat agttagcaaa
      721 agtttattgc ctttagtttc agtatttata gaacctggaa aagtattatt cttaaacaat
      781 gcaataaatc atggtatatt agggcctata ggtatagctg aggcaaaaga agcaggtaaa
      841 tctataatgt tcttactaga aagtaaccct ggaccaggtt taggagttct attagcatat
      901 tggatgttta gtagaggaag tgtaaaacaa tcagcaccag gagctgtaat aatacatttc
      961 tttggtggta tccatgaaat ttacttccca tatattttaa tgaatccagt tttaatattg
     1021 gctacaatgg caggtggagc agcaggaata ttgacttttt caatattagg agctgggctt
     1081 gtagcagcac catcaccagg aagtatattt gcacttatgg cactagctcc aaagggagga
     1141 ttacttccag tattagcagg ggttgcagtg gcaacagttg tatcattctt agtggcagca
     1201 ccatttgtta agagagcttc agcaaatgaa agtgaagaag acagtacttc tttagaagaa
     1261 gctaaagcaa aaatgagtga tatgaaatct gcaagtaaga actctgagaa aaatattgag
     1321 aaaaaacagc ttgaagtaaa tgagattaag aagattgtat ttgcatgtga tgcaggaatg
     1381 ggttcgagtg caatgggagc ttctagattt aaaaatagaa taaaaaactt agatttagat
     1441 atagaaatta caaattcatc tgttgataat cttccagatg atacacaaat agtagtaact
     1501 cacaatacat tagttgaaag agttgctaag aacaattcaa gtgttgagat agtttcaata
     1561 aacaattttt taaatgaccc aaatttagat acattattta aaagattaga atctaaataa
     1621 aaataaaaag taccactaat taggagtgga taatttgaaa aaaaagaaga ttacatctag
     1681 gcaaaaaaag ataatactaa tgatagtaga aaattcaaag aaaaacattc caataactat
     1741 ttctgagatt gcagggacgt tagagttaag ttcaagaact gtacttagag atatgtctgg
     1801 aattgaaaaa tggtttgatg aaaatgattt taactttgta aaaaaaccag gagtaggatt
     1861 gattttagag gaaaatatag aaaatcaaaa ctttataatt gagttgttag aagaagaaaa
     1921 aatagaaaaa gaatacagca aggaagaaag aaatttaatt atattatcaa agctactagt
     1981 ttctaacgag ccagtaaaat cttattattt tgcaaaaata ttaaaggtat ctgaaggggt
     2041 actgaataat gatttttcat tagcaagtaa atggttggaa agatttgata ttgagcttgt
     2101 tagaaaacca gggcttggtg tctacctaaa aggtcaagaa aaaagcttta gagaggcata
     2161 tgtaaatctt atatacgatt cattcaatga aaaagaaata ctagatatgg tcagaaatat
     2221 aagtgaaaat atacaaacag ataaagcaat agaaatttta agtgaaaata gacttttaaa
     2281 tttaatggat aggtgcataa taataaaagt tgaagctaca ttaacaaaga agctttcaga
     2341 tttagatgta aatttagctg atagtgcata tatagggctt gtagttcata tttcattagc
     2401 cttacagagg ataaaaaatg gtgaaaatat aactatggat aaagagtttc taaaagaatt
     2461 atctataaca gaagagttta aattagctaa agaaatcgta aaaggtatgg aaacagattt
     2521 taacatggat ataccagtag atgaggttgg atatataact atgcatataa gaggtgcaaa
     2581 gcaaaggtca tcttcaaatc ataaggcttt aaacttggat gatatagaaa taatggaaat
     2641 tacaaataag atgatagatt tggctgaaga tgaatttaaa ataagtttaa aaaatgacga
     2701 gaggttattt aaagacctag caaatcactt aggaccttct ataaatagat tgaatatggg
     2761 attagaaata agaaatcctc ttttagatga aataaaaagc aagtattcat atgcctatga
     2821 tggagttgaa aaaatttcta gaattataaa agataaacta aatataaatt caatacctga
     2881 atcagaaata ggatatattg caatgcactt tgcatcagcc atagaaaaaa atcttatgat
     2941 gaatactaat ataaatattg ttgtggcatg tcccactgga ataggtactt caagattctt
     3001 atcaactaag attgaaaata aatttcctaa tttaaatata cttgaaacca tatcagctat
     3061 aaacatagat gaagagtatt taaagggaaa agatgttgat ttaatagtgt ctacagttga
     3121 gttaaataca agtttaaatt atatatgtgt aggtccattt atgagtttag atgatgaaca
     3181 gataatcaag gaaaaaataa aatctatagc tcaaaataaa ttaattaatt taaatacaaa
     3241 aaatgatact aaaagtaaga ataaagtata tgaacagata acagaaagta tgaatatagg
     3301 aaaagatatt cttcaatttt tagaggaaat tagatttgaa aaatttgaaa gtaaagattt
     3361 aagtgagctt attgaagact catcaaaaat atttgcaaaa tctagtgaag atataatgtc
     3421 tataaaagaa tcgttaaaag aaagattaaa aatctcaatt ccatatatag aagaatcaaa
     3481 aattctatta ttacattgta tgagtgaaag aatagatatt atgaagttat caataataaa
     3541 actggagaat cgaatcgtgc tagattcaaa tgaagaaatc gacaatgtag tttttatgtt
     3601 acttcctaaa aattcaccaa gttatcaaag acaaataatg agtgaaataa gtggttcatt
     3661 aatagacaat ataattttta ctaataagat aaataaattc tctatagaag agatgacatt
     3721 agaggtaaaa gatatagttt ttaattttta tacaaacaga ttaaaagcat ttatagatga
     3781 ataaaattaa tattaaaaag gaaggttgat actatgagta aagtattgaa tgagaataat
     3841 atatttttag ggttagatag tgtttcaaag gaagaagcta taactttagc aggaagaaaa
     3901 ttagttgaaa atggatatgt aaaagaagaa tatataccag caatgctaga aagagaaaaa
     3961 gtaatgacta catatatggg aatgggagtt gctattccac atggtgtaaa tgaagcaaaa
     4021 aaagaaatat tatcttctgg aatagttata ctacaattcc ctaatggtat agattttgat
     4081 ggagaaaaag catacttatt aataggaata gctggtgttg gagatgaaca tttagaaata
     4141 ctatctaata tagcagtagt tttagatgat gacttaacag aaagactaaa aaattcaaat
     4201 gacaaacaag cttttatgga agcttttgcc aaataatctt aggagggatt taaaatgaaa
     4261 aaggcaatcc agtttggagc aggaaatatt ggaagaggtt ttattggagg cttgttagtt
     4321 aagtcgggtt atcatgtagt atttgctgat gtaaatgagg agatattaaa ctcaataaat
     4381 aaagataaaa agtatactat acacataaga gatgtagaat gtatagatga agtaattgat
     4441 aatataagtg cggtttcatc tataaaagaa gaaataatag atgagattgt acaggctgaa
     4501 ataataacta cagcagttgg acctttagtt ttgactaaaa tagcctctac aattgcaaaa
     4561 ggtataaagg ctagaaaaga aaagggttta acaagtaatc taaatataat agcctgtgaa
     4621 aatgcaatat acgcaagttc ttctttaaaa gaagaggtac ttaagtattt aaataaggaa
     4681 gaagtagaat atttagagat atatgttggt tttccaaact gttcagttga tagaatagtt
     4741 cctcctggta aaaatgaaaa tcctcttgat gtaacagttg aaaattttta tgagtggaat
     4801 gttgaaaaac aaggatttaa aggggaaata ccaactatag ttggaatgaa tttagctgac
     4861 aaccttatgg cttatataga gcgtaaattg tttacactga atacaggtca tgctataaca
     4921 gcttatatag gatatttaaa aggatataag actatagaag agagtataaa agataaattt
     4981 atctgtgata tagtcaaaag tgcaatggta gaaagtggag aaggattgat taaaaaatac
     5041 aattttgact cagaagcgca ctataaatat attgataaaa ttttaaatag atttaaaaat
     5101 ccatatctta atgatgatgt attaagagtt ggaagggaac cattaagaaa actaagtgat
     5161 aaagatagat tgattaaacc tttaatgaca gcaaaatcat atggattatc agttgacaat
     5221 ttgattcttg gaataggtgc agcgcttcat tataataata gtgaagatac tcaaagtgta
     5281 gaattacaag agttgataaa gtctaatggt gtaaaaaaag ctgttgctaa aatagctaat
     5341 ataagcaatg acgaagaact tttaaataat atcgaaaaaa gttatatatt tatgaaaaat
     5401 ttataaattt acttgaaaaa aagtagttct gataaagtaa ctataatata ttattctaca
     5461 cagtttgaat tatttgttga ataatagaga attataatat ataattaaaa ggaataaatt
     5521 aatatattta cctgtatatg tttgataata tggttcaaat gtttctacta agctaccatt
     5581 aatagcttga ctacaggagt gaagaagata tagtttatac ttattttaaa ttatatatta
     5641 agttaattac tttgaatttt aagctcctgt ctatgatata gacaggagct tttttagtat
     5701 tttaatataa aatacttaca agaatagata gcaaaattag aacaaatata atttttttat
     5761 taatataact ttagaaaggg ctaggtggaa gttcaatgaa ggctttacaa gaaaagatat
     5821 tgagagaagg cagtgtttca ggaaatgata tactaaaagt tgatagtttt ttaaaccatc
     5881 aaattgatgt agctttttta aatgaaatag gaagagaatt taaagagaga tttaaagaag
     5941 aaaaggtgga taagatattt acaatagagg cttcaggaat agctatagca tcaattgttt
     6001 cacagtattt tgataatgca cctgtagtat ttgcaaaaaa atcagaatct aaaaatttgg
     6061 ataaagatgt atatgagact aatgtatatt catttactaa ggcaagagaa tattcagtta
     6121 aagtttctaa aaagtacata aacaaaggtg aaaatatatt gatagtggat gattttttgg
     6181 caaatggaag agctgctctt ggacttaaag atttaattga gcaagcagaa gctaatttag
     6241 ttggagtagg aattgtcatt gaaaaaggat ttcaagctgg aggagcactt ttaaaggcta
     6301 atgatgtaag attagagtct ttggcggtag ttgaatctat agataatgga actgtaaaat
     6361 ttaggtaatt tatgatatta tatacttgtg tttagcacat attttaacta tgtaattatg
     6421 gaggattttt tatgatattt tttatagaca cagccaatat tgaagaaata aaagaagcaa
     6481 atgatttagg tgtaatatgt ggtgtaacta ctaatccatc tctaatagct aaagaaggta
     6541 gagattttat agaggtagtt aaagaaataa gtgaaatagt agatggacca ataagtgcag
     6601 aagtaataag tttagagcat gaaggtatga tagaggaagc agaaaaatta tctaagatac
     6661 ataaaaatat agttataaag atacctatga cagcagaagg tctaaaagcg gtgaaagtac
     6721 tatcaagtaa aggtataaaa actaatgtaa cacttatatt ctctgctgga caagctcttt
     6781 tagctgctag agctggagct acatatgtaa gtccttttgt tggtagattg gatgatatat
     6841 cacaaaatgg tcttgattta attgaagaaa tcgttgatat atttagtgta aatagtattg
     6901 aggctcaaat aatagttgct agtgttagaa atccaataca tgttttacaa gctgctagaa
     6961 tgggagcaga tatagcaaca gttcctctta aagttataaa ccaaatgatt aagcatcctt
     7021 tgacagacaa aggaatagat agctttatga aggattggga aggtgcttct ttaaaattat
     7081 aattataaac tacgctttat aagtatatta aagaggttat tatggaaatt ctaagtaata
     7141 aggctaggct attttgccta gtcttatttt ggtgtataat atattgaaac atattattga
     7201 aatagattaa ttttttaagt gaggaggcga aaaatggata aaatattaag agagatacaa
     7261 agagaagata aaaaaaatcc atatactgac caagaacttg ctgaaatgtt aaatgtagct
     7321 aggtctgaag tgatatcagt gaggaaaaaa aataatattt tagattcgag agaaagaaga
     7381 aaaaaaatat taataaaaga tatatcaaaa attgtaagtg aaaatcctca aatgtctgaa
     7441 agaaaaataa ctgagatatt aatagagaat ggatataata tatcgagaag tgcagtttca
     7501 aaattattaa aagaagaaaa tttaaatcag tcaaagattg ggattaaaga taaagagcca
     7561 gcatcatcag gaaagtatgt gaaggtgagt aaagttgtag aagaagatgg atttgaagaa
     7621 ttgataggta taaagggaag tttgaaagaa aaggtaaatc tagctaagtc agcaataatg
     7681 tatcctccta atggccttca tactataata tatggagaaa caggggtagg taaaagtgaa
     7741 ttagctacat gtatgtataa atatgctata aaaaacaaca tcaaagaaga gaacagccca
     7801 tttatagtgt ttaactgtgc agactatgca gaaaatccta atctattgat agctcaactc
     7861 tttggggtag taaaaggtgc atatacagga gctgatgcaa atagagaagg tcttgtagaa
     7921 caagctaata atggaatatt gtttttagat gaaattcata gattgcctgc tgctgggcaa
     7981 gaaatcttat tttcattaat agatagagga gaatttagaa ggttaggaga aagtagttct
     8041 aatagaaagg caaatgttct cataataagt gctacaacag aaaacccaga ttctaattta
     8101 cttcaaacat ttagaagacg tataccaatg gtaataaatt tacctaatat gagtgaaaga
     8161 ccaaaatctg aaagatatga aataataata aagttttttg aaagagaagc aaagcgagta
     8221 aacaagaatt ttataataac taaagaagtt atgtgtgcac ttatgaatta taagtgtact
     8281 ggaaatatag gtcagttaaa gagtgatata caagtaactt gtgctagagc ttttagtaaa
     8341 tcaatatttt ctagcgataa ggtaattata gatttagata gtttaaggga ttatataaaa
     8401 agtggatatt atgattgtaa ttgtaaaaat gaaaagtatt ctgagttttc agttgaggat
     8461 atatatatag atatcagtga attggtgagt ggaaaaaata gaattttatc tgataatgag
     8521 ataagcgaga tatataatta tgcagaaaaa gagcttaagg ttttggaaag taagtgtttt
     8581 tcagaagaag agttaaaaaa tgcatttata gaaaagttag ataagaagtt taatgctatt
     8641 aaaaacaata aaaatttatc tgatagaaga agtcgaatag actggggagg acaattaaaa
     8701 gagtctacat tggagataat ggataaggta attgtattta ttaaggagca gtttaagcat
     8761 gtaaatcaag gattgtactt ggctctagca attcacattg agcatgctat aggtaggata
     8821 aaagatggaa aaactataat aaatccgagt ttagataaaa taaaagcatc aatgccaatg
     8881 gaatatgaat tgtcaagata tataatgggt atagtggaag atttgacaca agtaacattt
     8941 ccagaagatg aacttggata tttggcttat tatataaata aattttgtta taatgaggaa
     9001 agtataaaag ataaagttaa agtagtaata gttacgcatg gtaaagttgg aattgaaatg
     9061 tctaaagttg taaatcatat tttaggtata gaatgtacac taggtataga aatagcactt
     9121 acagattcac catcagaggg gatagagcat gtattagaag agcttcaaaa gatagaagct
     9181 agaaaaggta ttctagttct aattgatatg ggttctttgg taattcttgg tgatgaggtt
     9241 gaaaaaagac ttggaattag atgtaaaact gtaaacagag tagatacgtt attggcaatg
     9301 gaagcaggaa aacttgcaac cattgaagga aaaagtttag atggtataat agcagattta
     9361 aagaaaaata agaattatgc catgataaat acaaataaat ttagttacag aaaaaacgag
     9421 tatggaaaga aaaatgtaat cattacttta tgtttgagtg gtgtaggtac agctttaaat
     9481 ttaaaagagc atatagaaaa acaaattaag gaatatgaca ctttaattga ggtaaagcca
     9541 gttgcttttt taaataataa tttagaagaa gagttaaatg atattgaagc tgaatataat
     9601 attattgcta tctgtggaac tatcgatata gattataaaa acgtaccttt tatttcttat
     9661 aatgaggtac ttggtaaaaa tggtattaat aaagtattag agcatttaaa cagtaaacaa
     9721 ccagttttaa atattgaaaa ccagttgaat aatttaatac atgaagattt gattttatac
     9781 aattttgaag gaatttcaaa ggagtatata attgatactc ttgtttcaaa gcttgaagaa
     9841 ggtggatatg ttgactcaaa gtatatatta agtgtatata aaagagaagc aatgggaagt
     9901 gttgtaatgg cctctaaggt agccgtgcca catggacttc cagaaaacgt tataaagcct
     9961 gcaatagcaa tcgcaaggtt gaataagcct atagtgtggg acaataaatt tatggtggat
    10021 ttggtagtac tactggcact taaagaaaat aataagaaag aaatacgaag cttattttca
    10081 aagattaatg accaacatac tttagaagtt ttattaaata ctgaagataa aaatgaaata
    10141 aaagaacttt taagctaatt attagtacag agaagaaaga caaaaaattt gctttagtat
    10201 aaaatttaaa actacttaat tgctaagtag ttaattactc gatataaggc tagttctatg
    10261 aaatttattt atatgatttt atagaattag cttttttctt ggtaaataaa atatatgaat
    10321 ttatttatgc aaaaagtaaa ttttattgga caattctatg agaatt
//
LOCUS       DAMJWU010000115        10287 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMJWU010000115 DAMJWU010000000
VERSION     DAMJWU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.119"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            169..948
                     /locus_tag="PC460_003158"
     CDS             169..948
                     /locus_tag="PC460_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2602020.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1258..2079)
                     /locus_tag="PC460_003159"
     CDS             complement(1258..2079)
                     /locus_tag="PC460_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF2602021.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGNVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEIWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(2754..3572)
                     /locus_tag="PC460_003160"
     CDS             complement(2754..3572)
                     /locus_tag="PC460_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2602022.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQVFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            3740..4321
                     /locus_tag="PC460_003161"
     CDS             3740..4321
                     /locus_tag="PC460_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2602023.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4318..4512
                     /locus_tag="PC460_003162"
     CDS             4318..4512
                     /locus_tag="PC460_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602024.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(4676..6418)
                     /locus_tag="PC460_003163"
     CDS             complement(4676..6418)
                     /locus_tag="PC460_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HDF2602025.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            6847..8223
                     /locus_tag="PC460_003164"
     CDS             6847..8223
                     /locus_tag="PC460_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF2602026.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8510..9181
                     /locus_tag="PC460_003165"
     CDS             8510..9181
                     /locus_tag="PC460_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF2602027.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9370..10167
                     /locus_tag="PC460_003166"
     CDS             9370..10167
                     /locus_tag="PC460_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF2602028.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDVNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         3800 a         1241 c         1570 g         3676 t
ORIGIN      
        1 gattctataa tattaacact attttttatg ctatataaca attattgaaa atgatataaa
       61 tataatatct caaataatat gaatataaca tctgaataga gggtgtttta tttgtaaaaa
      121 ataagtttac agtttgagcg tataaactta tggtaaagga gaataattat gagttataag
      181 aagattgcaa ttataacggg tgctaatagt ggttttggta aagagtttct aaagttatta
      241 ataaatgaag aagaaataac agaaatatgg gcagttgcaa gaaacaaggc gagattaaat
      301 caattagtag atgaatttgg aagtaaggta aaaatttttt caaaagattt atcaaaaata
      361 gaagaagtaa aagaaattgg tacattttta agtaaagaaa atgtatgtat aaaatacctt
      421 ataaacaatg ctggttttgc aaaattctgt tcatacaatg atttaagtat agatgaatct
      481 attaatatga ttgatttaaa tattagtgct gttgtagcat tgggacttat atgtattcca
      541 tatatgcaaa agggaagcca tatcatgaat atttcttcac aagcatcatt ccagccattg
      601 ccatatcaaa atatttatag ttcaactaaa gcgtttataa gaaattatac aagagcatta
      661 aatgttgaac ttaagagtaa agggataaat gcaattgctg tatgtccagg ttggatgagt
      721 acaaatctat ttaagagagg tatagttgta gctgaaaaag ggacaaaaaa tttcccaggc
      781 atagtgtctc cagacattgt tgccaaaaaa gcattgaagg atgcaaagaa aaataaggat
      841 atttctgtgt atggtataaa tacaaaatcc agtcatttat tagcaaaact attgccacaa
      901 aaaatgatga tgaaggtttg gttaatgcaa caacatatta aggaataaga gtttcggtaa
      961 ttaagtatca gttggagatt caatctgtta tatattatga gaagttttgt tgagcaaata
     1021 ataattgtga caaaacggct tatctagtgc cgataagccg tctataatgt atataaaata
     1081 tatttttgat tttactttat ttagatattg aagtattaaa atatgtagga cttgaaattt
     1141 tataaagata aatactatga tattttgtaa aaactgagat atgttgatat tacatagttt
     1201 aactatactg ttaaattgaa gttttcttat tttgcattat gcttattact taatgattta
     1261 gactataatt tccataccat catatgcaaa ataaatgtta tcaagtttct tttctaattc
     1321 actgtaataa ccatatgatt ttccccatat ttcgtcaata tgtgtaatga taatcttttt
     1381 aattctataa tagtttttaa tttcaacaat ctcatctaaa gtaaatattt catctttaaa
     1441 tggtgtgtct ttaagttttg taccatcatt taatatacca tcatcagaaa ctagacttat
     1501 tattaaaaca tcagcatcaa agtacatatc actatgagta aatggcttgg gattacagca
     1561 ggcatatatg actttttttt cattctcttt gataacatag aaggttatat tcctgtctgg
     1621 gttattgtta atcaaatcaa tatcaaaatt atttatcttt acatgcgaag tttcattaat
     1681 agaaatgcaa tttataacat taccataata tttaagacaa tcgagattcg ttcggtttat
     1741 atcttcaatt acttcaggaa gcgcataaaa gccaattgga ttattttctc ctgtgataaa
     1801 gtcacatcct attttttcga caattctaat acccttaaca tggtctgggt catggtgaga
     1861 aattgaaata tgttttactt ccgatatttt ttgtctgtta caagctactg caatatcttc
     1921 aggtgtgtct atcagcattt ttatatcatc aatatataaa cttggcccta atcgttcata
     1981 tcttccgcct ttttgtctgg cttcttcaca tattttgcat ttgcaaaatg aacttggaat
     2041 aattgacata ccgccactac caacaatttt taatttcaaa gttattacac caccttctta
     2101 aaatattaga ttatataaat aatctacaat cacaactgat taacaaaatt atgagttaat
     2161 aaattatatc acatgtaaat atgaaagaat agtatgaaat ttttataaac atatattaaa
     2221 tgtttctcaa tgaaattgac tttgtatatg aactgaatga cttattcaat atggataaga
     2281 catttttctt gtaataagat atgattgtcc tataaggttt atgtaatttt gatataattt
     2341 gatataatat attgattaac aacatacatg ttattggtga gcatatttct atagaatgga
     2401 atatataatt ttttatattt atcattgtat tattgtctaa agaaagttaa aaagagtaga
     2461 gcacaaagga attatttgta ctttactctt ttattgtttt gttaaaaaaa ctaatttata
     2521 ttagtgatta aaatatattt aactagagta taaggatagt atataatctt ataattgatt
     2581 tatctgacaa attaagataa tatcctaatt aaattgtaga tatttataca taacactaaa
     2641 agtaatttgt ttaacaaatt aagataatat cctaatcaaa ttgtaggtat ttatacataa
     2701 cactaaaagt aatttgtcta acaaattaaa atattatcca attaatataa aaattacttc
     2761 tttttaactg ggaagcatac ctctgttacc caattatcag gattactatc gttgccaggg
     2821 cttacatgat aaatattaaa cataggacca tccacatcat agttattatc tgatatccaa
     2881 ttaccaatag cctcacaagc gtctgcaact tgattgaaat ttccattaac aattgcagtt
     2941 gcagcagtca cacttggaac agtcttaaat ttaacatgtt cagtatcttt atactttcca
     3001 gatacagcaa cttgtacttc aacatctaca taatcttctt tatatccagt atcataaaaa
     3061 actgctttag aatactttgg gggttcaatt tgaacattct ggtcttttgt ctctaaaaat
     3121 acttgatgcc ataacattcc ttcgtcttgg taagttggta taacccttct aagtgtcatc
     3181 atatattttg gtgcaaaatt ctttatagtt acatcataat ttttcataat atcatttcct
     3241 ccaattcttt taatagtggt ttctattttt aatagttttt tctgtgtatc ttctaattgt
     3301 tcttttactt gagaatggtg gatatttaga tattttatga gactttcttt atcgttatat
     3361 tctgtcaaaa tttcttttat agaatacaag ccaaagccca tatcttttag tgcatggatt
     3421 ctgtttgtag tagaaagttg gtctgcacta tagtatctat atccattagt tttattcgta
     3481 tgagaaggag ttaaaagtcc tatttcatca taatgacgta acatacgaat actaatttta
     3541 cttagctttg aaaaatcgcc tatcttaaac atgtttttgt ctcctttata aaggtacttt
     3601 tagcttaata ttaatataaa ccatatcata atgttagagt caactaaaat tataattaaa
     3661 ttatattgac atatgatgaa aaaaatatta tactgaacat gttcagaacc gaaaatgtta
     3721 attgttttgg aggtctatta tgaaaagtaa tgataaagat gctagagaac gtataattga
     3781 agttacattg aatcttttaa atgaagtaga tgatattgag gagattacag ttagaaaaat
     3841 tgctgagcgt gcaaatgttg gagttggttt aataaattat cattttaaaa caaaggataa
     3901 tttgttgagt actgccatag gagatgtgat gtcaaatata atagcagagc tatatgacga
     3961 tagtgtatat actctaagac ctattgaaga tttaaaaaat ttattaaaaa aattgtgtga
     4021 tactggatta cattatgaaa aagtgcttcc ttttgtattg aatcaatgta taaccaatgg
     4081 agatatgcaa gcagaacttg atattgtccc tatgcttcgt aaaatttttg gaaataaaaa
     4141 agatgaaatg agtctaagaa tcattgccct gcaaattatt ttaccaatac agatttctgc
     4201 tttatctaca gaatcatttc agttatacag tggtataaat ataaagaata aatatgagcg
     4261 agataaattc attgatattt taatagaaaa tattattgga gaggatgttg atgtaagatg
     4321 aaaattataa ctattggttc aagtcttatc actgttttac tttttctttc aactatggtt
     4381 tgtgggttct ggattaagaa taataaggta acagatgcaa gttctattaa gttccatatg
     4441 aatagtgcta tttttacagg gatattttta ttgatttcca caatcctttt aattatatat
     4501 ataaaaaaat aataatatta ttaataaata aagtatcaga aattataaag acaagagtct
     4561 taaaaatttc tgatacttta ccatataatt ttattaaaca ttaattgtgt gtgaaaaaat
     4621 atcacttatt tcaatctaat actaaatctt aagagtgagt ataaagtatt agaaattaag
     4681 aagcattttt tagttcaagt ggattatcta taccaaaata atcatataaa gtagagctta
     4741 tttcctctaa atttccattt tggaaaggta attgtaaatt taccttttct accacatctt
     4801 taaatccagc atcagtattt tgatttaaaa aatccatata tacatcaaca gcttttctgt
     4861 aatcttcttg tgataattgc catatctgaa gtgatacatc agatgcaaga ccataactca
     4921 agtaataaaa aggaacttgg aagttatgag atacaagtat ccaaaaagga gcattttttt
     4981 ctctaagtat attattggaa actccatatt cttcctctaa atcaaaaaac aatttattga
     5041 tatcatttag actcatatat ggattcttgt atataatttc ttgaaattca tcataaagac
     5101 aagcatcaat aatagtattt aaaacaatcg ataagtgttc tttttttata gcttcagatt
     5161 gtttaccaaa aaaatcatca aaatatttat aaaatagaat ttctaaagat gttgaatgaa
     5221 cttcacatac atctatactt ggttgcatct tattatttaa gttaatactg ttgtagtaat
     5281 tataaaaatg accaaactca tgagcaaaac tagtaacacc taaaaaagaa ttgtcccatg
     5341 tattaaacaa gaaaggtgct ttatatttat ttatataagt agtatatcct ccaggtgatt
     5401 tgttttttcc agaagaataa tcgtacaagt catatttttt catatagtcg aagctctctt
     5461 ttagttttgg agatatatct tcaagtactt tatcaaactt cctaaatgag cgatttttat
     5521 atactttaat ataaatagaa gaatcagatg gtttagatga aatctctcta tataatggaa
     5581 ctatgtaatc ttttacatct tgtctaaatt ttttagcttc ttcttgagaa tagtctttgt
     5641 ttaggttcat ataggcataa tctatataat tcttaaatcc ttgcttactt gctatttcag
     5701 ttcttgtctg tataagttct aagaatatgt taccaaatat aggattgtat ttttttaagt
     5761 aatctgaata aattttcaca tattcttcag ggctaagatt tggtcttgac aaggcttctt
     5821 caaagtctac ctctatacca tctattgata ctgtagattt agctaataaa tcttcatatt
     5881 ttacaactag gtcttgctct tttttaagca aaccttcaac ttctttagaa ttaagttttc
     5941 caatgttctt tagataatta aatgtatcct ttccaagttc tttttctaat tctttagaga
     6001 atttagaagt tacaaatcta tcttgaaagt ctttgtatga catgtcaaca tctactgatt
     6061 tactcataag atacttatat tccttagagt aaaattcgtc tgatgaatct atgttatttc
     6121 taatttttgc tatagtaagt gtactgtaaa aatcctgata aattttatca acttcattaa
     6181 ataaatttaa ttgttcctta gctgttttag ctgttaaaag tttgtcgtta cacttgttaa
     6241 tgttttcgca aattgactgt atattaggac gcttatattc cattttttca aaaggaatag
     6301 ttcctctaac agaatctttt tttgtatcat tagtataatt tcttatgtat gatgcaaata
     6361 taataaaaac tgcaattaaa attagaaaaa caaatatttt cttcttggat ttaaccataa
     6421 aaatcctcca atcatggata tagttattaa ttattttact atattaaata taaaaaatgc
     6481 atgttaagaa aatttaacat aaagatatag ataagattat aaataatata aaaaatttaa
     6541 cttcaaaaaa gtatatgaaa tttatactgg taaaatgata aataaatttt aagtacaaaa
     6601 tagtaaaagc tatgatagtt ttagacctat tatatgtaaa aaatatagat gtttttggtg
     6661 caatttaaat taaaagatat atataaaaat ttaaaatatt ccaaacagaa attataaaaa
     6721 ttactatcat tgtctttata caatatttat actataatag aataataatt tagaggaaag
     6781 ctctaaaaat aaacaaatta agggtcttaa cagtagtgat tttatgttaa ggaggtataa
     6841 agaaagttga aaacaatagt tagaagactt attctctacg cagatggaat gcgtcctact
     6901 caaataatgg tatctgggtt tgctgcgatt attgtaatcg gagcattgtt gttaacactc
     6961 ccgatagcgt cgcagagtgg agagagtata ggcttattaa atgctctatt tacggctact
     7021 tcagcggttt gtgttactgg tctagttatg gtagatacag caacttattg gagtttattt
     7081 ggacagatag ttataataac attgatacaa attggtggtc ttggatttat gaccgtagcc
     7141 acaatgtttt cacttatggc acgaaaaaaa atacaactaa gagaaagatt attaatacaa
     7201 gagtcattaa atcaagctga tttatcggga cttgtaagac ttacaagatt tgtattaata
     7261 ataaccataa caatagaggg aataggtgca ctggttttat caactgtctt tattccacaa
     7321 tttggtttgt ctaaagggat atggtatagt gtatttcacg caatatcagc gttttgtaat
     7381 gccggatttg acttgatggg cagtgtaagt ggcccattta catcgttaaa ttcttatgta
     7441 aacaacttta cagtatcaat gactgtttgc gcacttatag tgcttggagg gctaggattc
     7501 cctgttgtac ttgatattgt aagaaaaaga agattttcaa aattaaatgt gcattctaag
     7561 gttgtattgt tttcaactgc tacacttatt tttgtgggtg cattatttat ttttcttata
     7621 gaatttaatc aaaaagctac tatggcggac ttgcctttaa agggaaaagt actatcagca
     7681 atatttcaat cagtaacagc aagaactgct ggttttaata cgcttgattt agctacttta
     7741 cgagaaagta gtgtatttgt aatgataatt ttgatgttta ttggagcatc acctgcatca
     7801 actggtgggg gtataaaaac aacaacacta gctgttttaa tcattacagt tagaagtttt
     7861 ctatcaggaa aatcagatat agaagctttt gaaagaagat tagctccatc aactataaaa
     7921 aaatctcttg gtatatttgt aatcagtata tcagcagtta tttttggaac attgattatt
     7981 tcaataactc agcctaattt tacattagtt caatcagctt ttgaggttac atcagcactt
     8041 gctacagttg gttcaagttt agctggaact ccaaacttaa atgctttagg taagattata
     8101 ataataatct ttatgtttat gggtagagtt ggttctctta ctttatttat ggctatactt
     8161 tctggtggta gaagaaagag tcaaccaatc agatatgcag aaggtaaaat tatggttggt
     8221 taagttacca aggagtaata taaatttata atggatttag agtagcactg taaatctgtt
     8281 gataaaataa atgagcctag atgtaattat atgaagaaat aaaatcgact aacgaacaaa
     8341 attaattaat gaaataaaaa aacagggaaa taaaatacaa ataaaaaggt aaatcaaaat
     8401 taaattaatc tgtgtatata aaattatata ttgtgtataa gttttgtatg tgtaaacaat
     8461 taatttaaga gatattatat aaattgtgta ttagggagga agtttcgaga tgaagcaata
     8521 tatagtcatt gggtgtggga gatttggaag ttcagttgcg tctactatgc atcttttagg
     8581 acatcaagta atggcaatag acaaaaatga agattcagtt caaagtatat ctgacaaggt
     8641 aacccattca cttatagtgg atgttactga tgagcaagcg ttaaggtcat taggtttagg
     8701 taactttgat gtagcagtag ttgcaatagg ttctgatata agggcatcta taatggcgac
     8761 tcttatagcc aaagaaatgg gtgtagagtt gataatatgt aaggcaaagg atgaattaca
     8821 agctaaagtg ctttataaaa ttggcgcaga tagagttgta tttccagaaa gagatatggg
     8881 agtaagagtt gcacacaatt tagtttcaga taatatatta gaccatattg aacttgaccc
     8941 agagtattca attgttgaaa tcgtaactcc aaatagttgg gttggcaaga cacttataga
     9001 gcttgaatta agagctagat atgagataac tgtacttgct ataaaaacag gtaaaaatat
     9061 aaatgttaca ccttctccag atgaggaact tacagccgga agtatcctag ttataatcgg
     9121 tcaaaatact agtataacag cgataacatc tggaaataag gggataatta gaagaagata
     9181 atttactatt taatatatat ttaattgcaa tgaaagtaga tagtatcata taattatgaa
     9241 tagttatatg atactatttt tttaatcgaa ggtagtattt atttgtaaga ttagataaag
     9301 agaaattaaa ttaaaagtaa ggaggctgtg ctaatataaa aatttataag ttattagcat
     9361 ttagataaaa tgattacaaa tataaacagt aaggataatg aaaagttaaa gtatacaaga
     9421 gcactattaa aatcaaagaa taggaataaa gagtcaaagt tcataataga gggatacaga
     9481 atagtaatgc ttgcacttga atgtatggca aaccttgatt atgtatttat caatgaagaa
     9541 tttgaaaata agaaagaaca tgtaaaacta ttagaagatt tggataaaaa aaacacaaag
     9601 atatacaaga ctactaataa aaactttaaa gaattagtgg atacagaaaa tactcaagga
     9661 ataataggtg tagtttcatt taagaaaaaa aaattaagtg aaagtataaa taaaaaagat
     9721 aaatttgtat tgattttaga cagaatacaa gacccaggaa atatggggac tataataagg
     9781 actgctgatt ctgctggagt agatgccata atagcactaa agggatgtgt cgatatatac
     9841 aacccaaaag taattaggtc tactatgggt tctatttttg atgtgaatat aattgatgct
     9901 tcacaagatg aaactgtgga catgcttaaa tcattggatt ttaatatagt ttcaagttac
     9961 ttaaatacag aaaattttta tgacaaaata gattatggct caaaagtagc attggtgata
    10021 ggaaacgaag caaatggaat aaatgaagaa cttgtatcaa agtctgatat tttggttaag
    10081 atacctatat atggtaaagc cgagtcgtta aatgctgcga taagctctgc tatactgatg
    10141 tatgaaataa aaaaatactt aatttaatgt attgtaataa atataatgtc atgttataat
    10201 cttgaattaa atagtagagt atcaaaaata gtcaatatat attaaaaaaa taattaaatt
    10261 aattatatat tagatgtata ttatata
//
LOCUS       DAMJWU010000116        10246 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMJWU010000116 DAMJWU010000000
VERSION     DAMJWU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.121"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..249
                     /locus_tag="PC460_003167"
     CDS             <1..249
                     /locus_tag="PC460_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2602029.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIRTIG
                     FGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSVYKK"
     gene            265..1848
                     /locus_tag="PC460_003168"
     CDS             265..1848
                     /locus_tag="PC460_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2602030.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1876..3204
                     /gene="ssnA"
                     /locus_tag="PC460_003169"
     CDS             1876..3204
                     /gene="ssnA"
                     /locus_tag="PC460_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF2602031.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3349..4701
                     /locus_tag="PC460_003170"
     CDS             3349..4701
                     /locus_tag="PC460_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2602032.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4893..6002
                     /locus_tag="PC460_003171"
     CDS             4893..6002
                     /locus_tag="PC460_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2602033.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6285..7676
                     /gene="hydA"
                     /locus_tag="PC460_003172"
     CDS             6285..7676
                     /gene="hydA"
                     /locus_tag="PC460_003172"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2602034.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7670..10231
                     /gene="xdh"
                     /locus_tag="PC460_003173"
     CDS             7670..10231
                     /gene="xdh"
                     /locus_tag="PC460_003173"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF2602035.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTEDALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEEDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENVVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
BASE COUNT         3964 a         1236 c         2137 g         2909 t
ORIGIN      
        1 gaagaagatg aatttgttca agcagcgtat aagggattaa aagaagctgg aatagattca
       61 gaaataactc aatactcatt ctgtacgaat ggaagtcact acgcaggtga ggctggaata
      121 agaactatag gatttggtcc ttctaaggaa aatcttgctc atacaataga tgagtatata
      181 gaattagacc aattattcaa aggtgcagaa ggatattatg gtatattaaa atcagtttat
      241 aaaaaataat taggagatgg ttttgtgaga actttaataa aacatgggct tatagtagat
      301 ggaaataaaa caccagcata tgaaggagat attttaatag aaaacgagaa aatattaaaa
      361 atatcacaag atttaaacga agaagctgac aaagttatag atgcaaaagg tagagttata
      421 tgtccaggtt tcatagatac tcatagccat tcagacctag ttattttagt taatccatat
      481 aatgaggtaa agattagaca gggaattaca acagaagtgt tgggccaaga tggtatatct
      541 atggcccctc tacctcaaga gcatatatct tcttggagaa aaaatttagc aggtctagat
      601 ggtgaaagtg atgaaattga ttggaaatat gaaacaacag agaattattt aaaaatgatg
      661 gattacaatg gggtaggttt aaatgaaact tatttagtac ctcatggaaa cgttagaatg
      721 gaagctatgg gacttgaaga tagacctgct actaaagaag aaattcaaaa aatgtgtgaa
      781 atcacagaga gagaattaaa agcaggagct ataggattgt ctactggact tatatatata
      841 ccttgtgcat actcattgac tgaagaaatt atagagatgt gtaaggtagt agcaaaatat
      901 gacggagtat ttgtagttca ccaaagaagt gaagcagata caattttaac ttcaatggaa
      961 gaaatcatag aaattggaaa acaatcagga gtaaaagttc atttttcaca ttttaaggtt
     1021 tgtggtaaag caaactggaa gtatatacca caagttatag agcttttaga aaaagcagaa
     1081 aaggaaggaa taagagtatc ttttgaccaa tatccatatg cagcaggaag tacaatgctt
     1141 ggagtagttt tacctccttg ggcacattct ggaggaactg ataagttaat agaaagatta
     1201 agtgatgaaa acgaaagagc aaaaatgaaa aaagatatag caaatggaat agaaggctgg
     1261 gataatttta tagaatttgc tggaatagac caaatatttg taacaagcgt aaaaacagaa
     1321 aagaataaag atacaattgg taagagctta ttagaaattg gaaaaatgag aggaaaagac
     1381 ccattagatg caacgtttga tttattaaaa gaagaagaaa atgcagttgg gatggtagat
     1441 ttctatggat tagaagaaca tataatagga ttcatgaaaa gagacgagca aaatgtgtgt
     1501 acagatggtc ttttagcagg taaaccacat ccaagagctt atggttcttt ccctaaaata
     1561 ctaggaagat atgttagaga gttgaatgtg ttaacaatcg aagaagctgt ttataaaatg
     1621 actaaaaagg cagctacaag ctttagtata aaagatagag gcgaattaaa agaaggatat
     1681 tttgcagata tagttatatt tgacaaggat acagttagtg gttgtgatga ttttatcaac
     1741 tctatgcagt accctacagg tatagattac gtaattataa atggaaattg tgttgttgaa
     1801 gaaggaaaat acaataaaat taaagcagga aaagtactta aaaattaaat cttagcttta
     1861 aggaggaata aatttatgat tttagtgggg aatgggaggc taataagcca agattcttta
     1921 aatccctata tggaagatgg atgtgtagta ataaatgata atctaattga agatataggt
     1981 actacagaag aaatgaaagc taaatatcca gtacatgaat ttatagatgc aggcaagaaa
     2041 ataatcatgc cgggtctaat aaattcacat atgcatatat acagctcatt tgctagagga
     2101 atggcagttc caggaaaacc ttctgagaac tttttagaga tactaaataa tttatggtgg
     2161 agacttgaca aacagcttac actggaagat acaaaataca gtgcatatgc aacgtatata
     2221 gaatgtataa aaaatggtgt aacaacagtt tatgaccatc atgcaagtcc aaatgcaatt
     2281 gaaggcagtt tgtttacaat atcagatgtt gcaaaggatt tgggaattag aacgtgtctt
     2341 tgttatgaag tgtctgatag agatggtgga aatacaatcc aacaaggtat caatgaaaac
     2401 gtaaacttta taaaatacgc tcaaaaagat gatacagaca tgataaaagg aatgtttggg
     2461 cttcatgctg catttacact atctgatgat acattaaata aatgtgcttc agagatggct
     2521 ggtcttgata caggttacca tgtacatgtt gctgaaggac tagcagactt acaatataat
     2581 gtagataaat atggaaaaag ggttttagaa agattcaatg attttggagt actaaaagaa
     2641 aaaacaatag cagttcattg tatatatata agtgcagaag aaatcagtct tttaaataaa
     2701 acaaatacat ttgtagttca taatccagaa tctaatatgg gaaatgcagt tggatgttct
     2761 cctgcaatag agttattaaa aaggggagtt acagtaggat taggaactga tggatatact
     2821 agcgatatgt ttgaatcatt aaaagttgcc aatataattc acaaacatca cttaaaagac
     2881 ccaagagttg gatttgaaga atcacaaaca atgttatttg ataacaatag aaaaatagct
     2941 aaagcttatt ataaaaatga cctaggagta cttaaaaaag gtgcttatgc agatgttata
     3001 gtagttgatt atataccaca tactccattg acagaaaata caataggggg acacactatt
     3061 tttggaatga gtggaagaag cgtagataca actattataa atggaaaggt acttatgaaa
     3121 gatagaaagt taataaacat agatgaagaa agtattttag caaaaagtag agaattaagt
     3181 caaaatctat ggaatagaat ttaaataatt gatattaata tacaagtaag aaacaagaat
     3241 ttagatgtta aaaacagagc ttaaacaaaa ataggaattt aaatgaagta tataaataaa
     3301 aaatatacgg aatagaatat taaattaaat atagaaggga gacccaaaat gtctcgagat
     3361 aaaagcccta aaatagctcc agtagatgaa aggttacctg tatccaaatc ttgggtattt
     3421 tcactacagc acgttttagc aatgtgtgca ggagcagtag cagttcctat gatgatagga
     3481 agtgctgcac gactagatca tgctagtatt gtttttttaa taaatgcttg cttgtttatg
     3541 gcaggtatag gaacactcat acaaagtgtt ggtataggaa atattgtagg tgctaaaata
     3601 ccagtaatag aaggaactag ttttgccgct gtggcaggta tgacagcaat agcctcatca
     3661 tatagtgatt cacaagttgc aatacagaca gtatttggct ctgtaatagt agctggattg
     3721 ttttgtatat tacttgctcc agtatttgaa aaattaataa gatttttccc tagtgtggtt
     3781 actggaacag ttgttatggt aataggtatt tcactatttc cagttgggat aaaatggata
     3841 actgacaaca aattggaagc ggttgaacca aactcattag cattagcttt agcagtattg
     3901 gcaataactt tactttcatt taaattctta aaaggaattt ggaatagtac agctatattg
     3961 gtaggtatag tacttggtac actactttcc atggcagttg gaatggcaga ttttagtgca
     4021 gtaaatggag ttggttggct taacttaaat gtacctttaa aatttggaat gcctagattt
     4081 gaaccatctg caatactatc attgtgtttg attatgttgg tacttatgac ggaatcagtt
     4141 ggtaatatga tagctattca tgaaatggta gacaaagaag taacaggtaa aaatattaaa
     4201 agaggtctat ttggagatgg aatatctaca gcattatctg gaatattcaa ttcatttcca
     4261 ataactccat ttggtcaaaa tacaggaata gtaggtctta caggtataaa atcaagattt
     4321 atagcagttt acgcaggggt tatattgcta gtacttggat tcttaccaaa atttgcagca
     4381 ataataggag caatacctaa gcctgttctt ggaggcgtag gatttgcaat gtttggaatg
     4441 gtaatggttg gaggagtaaa aactttaagc aaagtgaaat ttgatggaac taaaaatggg
     4501 gttatagtag cagttagtgt tggtttgtct atgataccac taacaaatcc tgatttctat
     4561 cataatttcc caacttgggt acaaacaata ttccatagtg gaataacaac aggtagttta
     4621 tcagcagtgc ttttaaacat attcttcaat gaactaggtt ctaaaaaaga aaaaaataat
     4681 aaagttgaag aagcaaaata aaaataaaac aagtaaaaat ctatattgac ttaaagtcat
     4741 taagcattaa taaaaataat tcagagatat taataattca acaaaactat cttatatttt
     4801 aaataatata aaaaagtaaa taaataacga atacaaataa aagaatacaa tctaaatatt
     4861 aaaaatattt aatttaaggg agagataata atatggctaa tttaaaagtt aatttatatg
     4921 gaatggaatt tttaaaccca attatgacag cggcaggacc tggagcaaaa gatggtgctc
     4981 tctgtcaaga ggcagcaaaa ggtggagcag gagctatatg tacaaaaact atatctgtat
     5041 taccagctga tgtaccaaga ccatgtatgg caaaaactaa tagtggtttt ttaaacacag
     5101 aattatggtc agagcttcca aaagagcaat ggatagagga agaatacaaa aaggtaaaag
     5161 aaacagggct tccagtaata gtaagtatgg gatattcagc agagcaaata gctgaggttg
     5221 ctcctttagt aaagccttat gcagatgcag ttgaattatc tactcattat gtaggtacag
     5281 acgttgcacc aatagtaaat gctttaaaag ctgcaaaagc tgttttagat gtaccagtat
     5341 ttatgaaaat gagtcctcat actgacattc aaactattgc aaaagctgtt gaagaagcag
     5401 gtgcagatgg acttgtaatg ataaactcat ttggaccatg tatggcaata gatgtaaata
     5461 caggataccc tataatggga agtaaagctg gatacggatg gttatcaggt gcaccaataa
     5521 gacctctagc aataagatgt atatatgaag cttctcaagt tgtaaatata ccaataatcg
     5581 gtgttggtgg aatcacttgt ggaaaagacg tggctgaaat gatgatggct ggagcaagtg
     5641 ctgttcaagt atgtacagaa gcaatcctta aagggccttc tatctatgga aaaattgcta
     5701 aagaattaaa tgaattttta gatgagaatg gatatgctga tgttaatgaa ataaaaggat
     5761 taactcataa gaaaatggca gctagagggt ttagaactca ttcagtagct ccagtagtgg
     5821 atgataacaa gtgtatcaaa tgtaagcaat gtgtaacaag ctgtgtatac gaagctttag
     5881 aagttacaga taagttgaac atagatgcag acaagtgttt tggatgtgga ttatgtgtaa
     5941 ctagatgtcc aaaaggagct ttaagcatct gctacaatca agtagaagct atgaatgagt
     6001 aataattata tagaattttg gtaattaact tagagtaata ggtattagga taataaaata
     6061 tatcagataa acaaacacgc attatgcatt aataatacag tctaaagtta ataattttat
     6121 tatattgatt taactaaata ttagtttagc tgaatattaa tttaattagg tattggttta
     6181 atcttaatat agatttacta cgtataagtg aactaactag attactttaa gttaattttt
     6241 tcatataaaa taaattgata cattacaagt aaggtggagt ctatatggga attgttttaa
     6301 aaggtgggaa agtagtaaca agtgaaaaaa cttactgtgc agatgtaaga atagaagatg
     6361 aaaaaatagt cgatataggt cttgatatat caaaagaaaa tgatgaaata gttgatgtta
     6421 gtgattctta tgttatacca ggaggtatag atacacacac acactttgac ttagatacag
     6481 gcgttactaa gacagcagat aactttgaga caggaacaaa agctgctata gtaggaggaa
     6541 ctactactat attggatttt gctacacaag ctaaaggatg ttctttgaaa gacggattag
     6601 aagaatggca tgaaaaagct aaggataaat catattcaga ttatggcttt catatggcta
     6661 taacagattg gaatgactcg gtttgtaatg aaatggagga tatggtgaaa gagggagtat
     6721 cttcatttaa actgtatatg gcgtataaag gaagccttca agttgatgat ggtgtaatct
     6781 ttgaagcatt gagaaaagca gaagagatag gagggattat tggtttccat tgtgaaaatg
     6841 gggacataat atgtgaactt gtggataaag caaagtcaga aaataattta tctccaaaat
     6901 atcatcaatt aactagacca gctataatgg aggcagaagc tacatcaaga cttatgaaga
     6961 tagctgaagt tgcaaactct aaagtatatg tagttcactt aagctgtaaa gaatccttag
     7021 aggaggtttt aaaagctaag caaagaggtg tggatgtaat agttgaaact tgtcctcaat
     7081 accttctatt aaatgatgaa ttatatgatt tagatggatt tgaaagtgct aagtatgtaa
     7141 tgtcaccacc tttaagaaat aaagaaaata atgaaatatt atggaatgct attttagata
     7201 atcaaataga cactatagga acagaccatt gttcttttaa ttataaggga cagaaggaac
     7261 ttgggataaa cgatttcagt aagataccaa atggttctcc aggtgtagaa cataggatga
     7321 gtcttttata cacatatgga gtctgtaaaa ataaaatatc tatcaacaag atggtagaag
     7381 taacatcaac taatgcagca aagttgtttg gaatgtatcc taataaagga actatagaaa
     7441 taggcagtga tgcagatata ttagtcttaa atccaaacaa aaaatccaaa atagaagcaa
     7501 aaaaacaatt acaaaatgta gattatacac cttatgaagg gtatgaagtg gattgtcaaa
     7561 tagaacatgt ttatttaaga ggaaaagaag tagttagaga tggagaatta atttgtaata
     7621 ctccaagtgg gagatatata aataggcgtc taacaaaggg ggctagagaa tggtagaatt
     7681 tatattaaat ggagagaaag ttgttgcaaa agaaggcaaa aaacttttag acttccttag
     7741 agaagatatg aatataactt cagttaaaaa tggatgttca gaaggtgctt gtggaacatg
     7801 tatggttata atagatggta aagctactaa agcatgtgta tttaaaactg ataaattagc
     7861 tgggaaaaac ataacaacta tagagggatt atcagagaga gaaagagatg tttttgcata
     7921 ttcttttgct agtcaaggag ctgtacaatg tggattttgt attccaggta tggtaattag
     7981 ttcaaaagca cttatagacg ttaataataa tcctactgaa gatgatataa agaaagcttt
     8041 aaaaggtaat atatgtagat gtacagggta tgtaaaaata attaaagctg tcgatttagc
     8101 agcaaagatt tttagagaaa atatagatgt gccaaaagta aactgtaaag gtctagtagg
     8161 tgaaaatctt cctagagttg atgcagttgg caaagcactt ggatattctg aatatgtaga
     8221 tgatatgaga attgaaggta tgatttatgg ttcagcagtt agagctaagt atcctagagc
     8281 tttagttaaa aaaatagata tagaagaagc aaaagcttat ccaggagttg ttgcagttct
     8341 aacagctaaa gatattccag gaactataaa ggtagggcac ttaaagaagg attgggatac
     8401 tttaatacca gaaggtgaaa taactagata cttaggagat ggaatagttt tagtagcagc
     8461 agaaactaga gaagcgttag aagaggcaaa aaagctagtt aagatagatt atgaggaatt
     8521 aacaccatta agtactacag aagatgcgct tagagaagat gctccaaaga tacacgagac
     8581 aggtaatata cttgttaagg aacatttagt aagaggaaac gctaaagaaa agatagaaaa
     8641 ttcaaaatat gttgtaacta aaagatatac tacacctgct acagaacacg catttttaga
     8701 gcctgaatgt gcagtagctg gtccttatga agaggatgga gtaataatct attcaacaga
     8761 ccaaggggtg tttgccactc aacatgaatg tgcagatatg ttagggctac catatgagaa
     8821 ggtaagagtt ataaacaaaa tggtaggagg aggatttggt ggaaaagaag acatgagtgt
     8881 tcaacaccat gcagccttac ttgcatacca tactaagaga tttgtaaaag tacagttaac
     8941 aagaaaagaa agtattattg ttcatccaaa acgacatgcc acagaaatgg aatttacgac
     9001 agcatgtgat gaaaatggat acttaacagg tatgaaggca acgataatat ctgacacagg
     9061 tgcatatgcc tctcttggag gaccagtgct acaaagactg tgtactcatg cagcaggacc
     9121 atataattat caagatattg atataacagg aacagcagta tatactaata accctcctgg
     9181 aggagcattt agaggatttg gagttacaca gagttgtttt gcaactgaat gtaatctaaa
     9241 tttacttgct gaaatggttg gaatatctcc ttgggagata agatataaaa atgcaataag
     9301 acctgggcaa gttttaccaa atggtcaatt tgcagatgaa ggaacagcaa tggcagagac
     9361 actagaagct gtaaaagaag agtatgaaaa gcatccacgt gcaggtatag cttgtgctat
     9421 gaaaaatagt ggggttggag ttggtatacc tgatgttgga agatgtagat tggtgataaa
     9481 agatggcaaa gtccacataa gaacaagcgc ttcttgtata ggtcaagggc ttggtactgt
     9541 tatgactcaa gtagtttgtg aaactgctag cctacttcct gaaaatgtag tgcatgaagt
     9601 tccagatact catttaacac cagactctgg taatactacg gcttcaagac agacagtatt
     9661 tacgggagaa gctactaagg ttgcgtcaaa taagttaaaa gaagctttag actctggcaa
     9721 aactttactt gatttagagg gagaagagtt ttatggagaa tatgaaagta ttacagataa
     9781 aatgggttgt gacaaagaaa atccagtaag tcatgttgct tatggatatg caacacaagt
     9841 tgtaattctg gatgaaaatg gaaaattgga aaaagtagta gcagcacatg atgttggaag
     9901 agctataaat ccaatcagtt tagagggtca aatagaaggt ggagtagtta tgggtcttgg
     9961 atatgctcta accgaagatt acccagtagt taactctgtt cctacagcta agtttggtac
    10021 attaggttta tttaaatcta cagatatacc agaaatacaa gctttaatag ttgaaaagaa
    10081 caaagattta ttggcttatg gggcaaaagg tgtaggagaa atttgtacag ttcctactgc
    10141 accagcagta caaaatgcct attatgtatt tgataaagaa tttagaactg aactaccact
    10201 acaaaatact ccatatagta aaaaaaagta acaaaaaaaa gaaata
//
LOCUS       DAMJWU010000117        10232 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMJWU010000117 DAMJWU010000000
VERSION     DAMJWU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.122"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..904
                     /locus_tag="PC460_003174"
     CDS             <1..904
                     /locus_tag="PC460_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2602036.1"
                     /translation="PLILDISFLIKEKYKYILFSFVIISSLIISLDKNIYLALESSSI
                     LLIITVLSIYIENENLSKLYSQNLYDKLRISKDELKKVNADLETYASSIEELAILKER
                     NRISREIHDSVGHSLSTTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAIS
                     ELKPVEYENYQSLFKIKELVKSFIKLTNINVKLTISKNTWNLSRNQSITLYRLIQESL
                     SNSSRHGKATEIRIFITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVE
                     FDNSNNGFTIRASFPKFSGGDFFE"
     gene            897..1544
                     /locus_tag="PC460_003175"
     CDS             897..1544
                     /locus_tag="PC460_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2602037.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1673..2608
                     /locus_tag="PC460_003176"
     CDS             1673..2608
                     /locus_tag="PC460_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602038.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIIEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2622..3719
                     /locus_tag="PC460_003177"
     CDS             2622..3719
                     /locus_tag="PC460_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602039.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQNSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3722..4786
                     /locus_tag="PC460_003178"
     CDS             3722..4786
                     /locus_tag="PC460_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602040.1"
                     /translation="MIRLTIANIKRYLKNHNLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNELDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            5030..5527
                     /locus_tag="PC460_003179"
     CDS             5030..5527
                     /locus_tag="PC460_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2602041.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5703..5951)
                     /locus_tag="PC460_003180"
     CDS             complement(5703..5951)
                     /locus_tag="PC460_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602042.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(6017..7033)
                     /locus_tag="PC460_003181"
     CDS             complement(6017..7033)
                     /locus_tag="PC460_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2602043.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7091..7858)
                     /locus_tag="PC460_003182"
     CDS             complement(7091..7858)
                     /locus_tag="PC460_003182"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF2602044.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7926..10115)
                     /locus_tag="PC460_003183"
     CDS             complement(7926..10115)
                     /locus_tag="PC460_003183"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2602045.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3562 a         1568 c         1184 g         3918 t
ORIGIN      
        1 tcctttaata ttagatatat catttttaat aaaggaaaaa tataagtaca tattgttctc
       61 ttttgttata atatcttctc ttattattag tttagataaa aatatatatt tggcactgga
      121 gtcaagctct atattgctaa ttataactgt actttctata tatatagaaa atgagaattt
      181 atcaaaatta tacagtcaaa atctttatga taagcttaga atttctaaag atgaattaaa
      241 aaaagttaat gctgatttgg aaacttatgc aagttctata gaagaattag caattttaaa
      301 agaaagaaat cgaatatcaa gagaaataca tgatagtgta ggacattctc tttctacaac
      361 aataatacaa ttaaatgcta tagaaaaact cttaaaagac aaacctctaa tttatgactt
      421 agtacatgaa cttagagaat ttgtaactga aagttttcaa gatgtaagaa gggctatatc
      481 tgagttaaaa cctgttgaat atgaaaatta ccaaagttta tttaaaatca aagaattagt
      541 taaaagcttt attaagctta caaatattaa tgttaaatta actatttcaa aaaatacttg
      601 gaacttatcc agaaatcaat ctataacttt atatagactt atacaggaat ccctttccaa
      661 ctcatctagg catggaaagg caactgaaat cagaattttt attacattca atacttcaaa
      721 tcttataatt actattagtg ataatggtat aggatgtggc aatataaaaa aaggaaatgg
      781 acttaatagt atcaatgaga gaatatatga attaaatggt aaagttgaat ttgataattc
      841 taataatggt ttcacaatta gagcttcttt tcctaaattt tcagggggtg atttttttga
      901 ataaaattaa agttcttata gtagatgacg aaaaattaat tagaaaaggt cttaaaatta
      961 tattatcatc ttataatgat ttagaaattg taggagatgc ttctaatgga tatgaagctt
     1021 tagaattttg taaaatgaat gatgtagaca tagttcttat ggatatacga atgaaagtat
     1081 gtgatggggt tctaggtaca aggcttataa aggaatataa taattctata acattattaa
     1141 ttttaacaac ttttaatgat gatgaatata taaaagatgc tatgaagttt ggtgcttcag
     1201 gatacctttt aaaagatagc tctgatgaag tattgcatga agggattcgt tcttcatttt
     1261 ttggcaatat tgtgcttgat aaaagtgtag ctgaaaaaat catgacctct gaaaaaacgg
     1321 ttaaacaaga gcatctttat gacatgtaca atttaacaga aaaagaaata tctataatta
     1381 gactaattgc aaatggactt aataataaag aaataagtca agaactattc ttatctgaag
     1441 gtacaataaa gaataatata actaatattt tagctaaact agaacttcgt gacagaactc
     1501 aactagcaat ctttgctttc aaaaataaaa ttgtaataga ataaatctaa aaatataaag
     1561 tgacttaagt cacttttttt agtgacttat agtaatagaa ttataattca caattcttta
     1621 ttataatatt atagcttttt aacttataca aaattatttt ggagggatta ctatggatat
     1681 agtcagagta aataatataa caaaaagatt taatgataaa ttagtacttg ataacatcag
     1741 cttttctgtt aaaaaaggtg agatatttgg acttattggc ccaaatggtg caggtaaatc
     1801 tacacttata aatataatta caaatcttat gttaccaaat agtggtagta ttcaaatcaa
     1861 tggtttagat ttatcaaaag attatataaa agcaaagtct ataatagggt tagttccaca
     1921 agaattagca attatagaaa ctcttactcc ttttgataac ttagagtact ttggagcttt
     1981 ctacggttta aagggaaaac tacttaaaga aagaatcatt gaagcattag aagttacagg
     2041 tcttactgaa gtcaaaaaga aaaaggtcaa aaaattatct ggtggtatgc aaaggagatt
     2101 aaatattgga atagcactct taaatcatcc taaaatatta atattggatg aaccaactgt
     2161 aggtgtagac ccccaatcaa gaaatcatat tttcaatttt ataaaagata tatctaaaaa
     2221 acatgaaact actgtaatat atacttccca ttatatggaa gaagttgaac acctttgttc
     2281 taaaattttt attatggatg aaggtaaaga aattgctttt ggagataatg attatttaaa
     2341 atcactcgta tctacaaata caaaattaat tatagaaata aaaaatataa atgcccaact
     2401 tatctttgat ttaaaaaata ctaaaggtgt tatatctgtt ttagaaaata attcattatt
     2461 agaattggat atagataaaa aattacaact tacagatata ctctctgtta ttgacaaaaa
     2521 caattcaaaa ataatgaaaa tttcttatga agaacctagc ctagaagacg tatttttaaa
     2581 tctaacagga aaaaatttaa gggactaatg gaggtgattt tatgaaatta tacatgagtg
     2641 taaaaactat gctaaagagt ttaaaaagct cctttatact taatttaata tattttttag
     2701 ctttgcctct gattttatct tggtttcttg ggatggttac agaaagcatg tttcaaaacc
     2761 ctataaaaac agaatctacc cccattgtta tatatgacaa agataacaca agattatcaa
     2821 atgatttgac aaagtatttg aaaaatgatt tatcttatat attaacagtt aaaaaagatg
     2881 attctaaggc agaattaaag cttactatac ctaaaggcta tgagagtagt ttattaaatg
     2941 aaaaatcaaa tactctaaac atagaaaaac ttggaactcg tgatgatatt gcaatattgc
     3001 ttcaagatat attagatact taccatgaaa aattttattt aaataattct cagaaaataa
     3061 gttcagaaga tttttctaaa ttatttaata aaaattctat tgatacttct ataataaata
     3121 acaatgttaa acagaactct tatgaatact ttgctttagt ttcactagga tttcttgtaa
     3181 taatttttat aatgaacaat attttgtcaa attatataag tgaatcaaag ggactttcaa
     3241 aaagacttta ttctatgcct ataactagag tacaattctt aatttatgac tttgtaggtt
     3301 tatggattta ttcatttata ttcttattat tatatgtttt attctttaga atacttggaa
     3361 taacatttaa aggtaatttt gccatattgc tcctactttg tgctttatca tcttatttta
     3421 tgacttcaat atctaccttt gtaaccagct tttttagtaa aaaatacggt acagcaatag
     3481 tttatgcatt actcttttta cagacaattt ttggaggtat cttcagtatg ataagtgata
     3541 catttactaa gcttacaagt ttatctccta cctatttaat aggagaattg tttagtaatt
     3601 atgaaacatt taaaactata gattcaatag gagatttaat tttcacatgt ttaattatct
     3661 ctacaatatt aattgcactt gctattgtaa aggaaaaata taaatggagg gaggtttaat
     3721 aatgattaga ttaactattg caaatataaa aagatatcta aaaaatcata atcttttaat
     3781 taatatggtt atgcttccta ttatactaat tttttcactt aattttttta tcaacaactc
     3841 aggaaatcaa tcaatgtact tttctccagt agcaattgta tctgactctt caggaaagta
     3901 tgaacataag ttaataaata gttctaaatt aaaagaaaat tcatttagat taaatgaact
     3961 agataaggct atgaatctat taaaaaataa taaggtttct gctgtattcg ttttagataa
     4021 aaatttttca aatagtatag ataaattaca acgacctgta gttaaatgct ttaaaattga
     4081 aaatggtggt ggtagtcttt gggcagaatc acaaatagaa tcttttatta ctaaatcgtt
     4141 aaaattgaaa gtggataaaa atatagatga taaattgaca aagacaaata taattgataa
     4201 caaaccagat aagaataaag gttcattttt agtagtcttc ttgatatgct attttatgta
     4261 tattaatgca gcacatcttg cttctgattt atttacatta aaaaaatcaa atgtgctaaa
     4321 aagacttatc tctacagata ataaagatat aaaaataatt ttcagtatat ttttaggact
     4381 attttttatt caatcaattg tatacacttt tgctctatta tgtttttcaa ttatagaaga
     4441 ctttaatttg agtctgaatg tattgatgat tgtactagca aatagttttg tttccactgg
     4501 atttgtattt tgggtagcta gagtatttaa aaatgaaagt tctataagtc ttatatctac
     4561 attttattct ttaataggtc tagctatatc tatctctagt ctaataccat caatggataa
     4621 attatccttt atgactaatc tttcaaagtt cacaccattt tattggacta tagatgctat
     4681 taaaaacaat ggtagtattt taataaatat aatagctcta atattaattg gaattatatt
     4741 tgtaactgca ggaagtctta aactaagaga ttttgctaaa aactaataat tattaaatat
     4801 aattagtatc tatttttata tagttgtaaa agaatatttc aacatattaa attattttat
     4861 cttatatttt tctctttagt ttcacacatc accaagtcta aaccttatat tatatattaa
     4921 tatttttgac ttgtcaagtt atttatgtta taatattaat cattagataa ttaaagcatt
     4981 gtacctaagt tgcaatatct aaaattcaat ttggaggtat aactagcata tgtataaaat
     5041 tggtgaaagt gtaatgtatc caaaagaggg agcctgctct gtaaatgata ttgtaacaaa
     5101 gaaaataaat catgagatgc aaaaatacta tgaattaagt gtcattttta atagtaacct
     5161 taaaatttcg attccagttc taaatgcaga tagaattggc atacgtccag ttatggatgg
     5221 aaatgatgtt gataatttta tccaatctat taataaaaca gatggtgtat ggatttttga
     5281 tagaaaagaa agacttaaac tgtaccaaga taaatttcat tcaggagatg tttttgaaat
     5341 tgtaaaatta atcaaaatgc ttatgataca agacagttca aaacaattgt gtagcacaga
     5401 taaggagttt ttaaataaag ctcaaaaatt tgctttatca gaattagctg ctgctcaatg
     5461 taaatcctat acaatggtat tagaggaaat gaaaaaacat atattaaatt caaagaatac
     5521 aaattaaacc agttttttaa actggtttta ttttttatac taatcatcca tgctacttta
     5581 gcttatttta aactgcctaa tctaatatta ataaagttat ctcaattaga tagatgtatt
     5641 ttattcaaat aaaaaaaact aagagtaaat catattactg atttactctt agctaataaa
     5701 atttaatctt cttcaagttc ttttttctct tgtaataata cacttaattc atttgcttct
     5761 aaaacttgtg cctctgcaat atcaagtaac tcttgttgcc attccacaca tttatctttg
     5821 taatcaaaag gtagcgttct gtcaactatg tacttatcaa tacagtcctc cgcactttta
     5881 attatttctt ttctttcatc aataaatttt tctaattcta ctaaggcttc ttcataagct
     5941 tttctactca tataaattcc ccctttgtac aattattcta cattaaaatg atttttcctc
     6001 ttttttgtat aaaattttat ttgtatataa cttttccatc aatatctttt atagtttgac
     6061 atccagttaa tatcatagaa ctacttagct ctgattgaag tttatttaca taagtttcta
     6121 caccatcagt tgcaccacca aaagaagcag tcacaaaagg tcttcctatt aatactgcat
     6181 ctgcacctaa acctatcatt tttagtacgt ctaaaccagt tctcactcca ccatctgcaa
     6241 gtattgtaac tttaccttta acagcatcag caatttttgg taaaacttca catgctcctg
     6301 gtgtacagtc aagcactctt cctccatgat ttgatacaac tattgcatcc acacctgctt
     6361 ctactgccat taaagcatct tcaacagtca ttataccttt caatataaat ggtagtttag
     6421 tagactttac taactccttt atttgtttaa catttttagg aagtactggt tttccatgta
     6481 atgataaagt tactagacca caggcatcta tatcaactcc aactgcaaat gcaccagctt
     6541 cttcagctaa tcttatcttt tttataatat tttcattatc ccaaggttta ataaatacaa
     6601 ttccagctcc attatacttt ttgactacat ctaaattttc cattaaaaaa gcatctactg
     6661 ccgtatcacc aaccattgca taaataccac tattagcaca tcctgcaact actggttcta
     6721 tgtaatctct ttcattaatt tttccaccca tatttaaagt agttcctgtt actggagctg
     6781 caaatatagg tgcactcatt ttctttccaa acatctctat agatgtatca ggatttgata
     6841 catcatgtat tactctcata tttactttta ctttttctaa actcttgcta ttttctataa
     6901 aagaagaacc acttccttta cctcccatgc caggcacttc acctgcacaa gcaaccccat
     6961 tacaaacctt acaaacttta caacttccat ttaaattttc tctagcactt tttaataatt
     7021 cattatattc catctttaat cccccattaa ctatagtaat ttatattata tacattatat
     7081 catattatat ttacagtata tttttcaaaa aactttttct gtggatagga gttatgccat
     7141 atttttcaat agcttcatag tgttctttag ttccatatcc tttgtgagat ttaaacccat
     7201 attctgggta tactctatca tattggtaca ttatactatc cctagtaact ttagcaagta
     7261 tacttgctgc tgctattgat atagattttg aatctccttt cacaattgga ttttgagata
     7321 tgtcaatacc tggtattgtt gctgcatcaa ctaataaata atccggtgct ttttttaaac
     7381 aatttatagc ctttttcatt gccatatatg tagcatttaa tatattaaat tcatctattt
     7441 cttcattgtt tactatacct attccataat ctagtgcttc ttcttttatt acttcaaaaa
     7501 gttcatctct ttttgcctca ctcagtttct tagagtcatt tacaccttct attttggtat
     7561 cttttttaaa tactacaaca gaagctacaa caggtccagc aagtggtcct cttcccgcct
     7621 catcaattcc tccaatatat aaataacctt tatcatagcc ttcgttctca aatatattta
     7681 tagtttccaa tctttcttct tcttttctaa tattatccag cttttttgct agttttatag
     7741 ctagaccttg tacagatttt ctttcatcaa ctcttaacag ctctatatat tccatatatt
     7801 tttctacttc taaagtctct attatttctt taatctccct cacactttta tcctgcatac
     7861 cattccctca tctttcgttt ttaaaaattt aatttttaaa tatattaaaa ttttttaatt
     7921 agagtttaat tgcatccact atagccacat tttaaacata tactacatcc acctgtatgt
     7981 gcaattcctt cacttccaca ctcaggacac acaaattttt catcttcttc aaaattttta
     8041 tttattattt ctttggcttt ttcatcttta ttatcttttt cttttttatt atttttataa
     8101 tattcttttt tactattttc ttttttatct tctttattat tttcttcctt tttttcattt
     8161 attaaaattc cactcctttt acatccatct ctaaatattg ttactccttt aagaccagcc
     8221 tcccatgcct taatatatat attgaaaaca tcttcaactg tagtttcaaa aggtatattt
     8281 attgtagatg atatagaagc atcaatatac ttttgccata ctttttgcat gtttattctt
     8341 tcttcatatc ctatattcat agcagtaaca aaaaaatctg gtaaacattc ttcatctgta
     8401 atacctttct tgtccatata ctctttaaca attggagtat atactttata atacacatcc
     8461 tcatcatgca agctttcagt ttttcttata tatgaaagat taaatatagg ttctattcca
     8521 ccactaatac ctatcattgt agatatagaa cctgtaggag gtatggttaa aagttgtgaa
     8581 tttctaagcc catacttttt aaccatttcc ttagtgtctt catttagatt ttctataaaa
     8641 aacttagatt tagaaatatt ttcatattta tactcactaa atgttccata ttctttagca
     8701 attagtgctg attgcttcac agcttcattt aacatagtct taccaattaa atcacatagt
     8761 tctaaagatt catcagaacc atatcttatt cccattttta taagcatatc agctataccc
     8821 attacaccta aacctatctg tctatactta tgtacactat ctctttgttc ttgtaatgga
     8881 tgcaagttca atccctcatc taagacatca tttaaagcta ctacagcttc tctaacgcaa
     8941 gattttaatt tattaatatc aaaagtagca tgttcaccaa atggttgtat tataaactct
     9001 gacaagttta ttgagccaag tagacaactt cctccacttg gaagtggttc ttctgcacaa
     9061 ggatttactc ctgaaaagca aaagttctta tcttcactca taaaattata tgtttttatt
     9121 ttatcccaaa acaatatacc tggttctgca taatcccagt tatttttcac aaatttatta
     9181 aataaatcat aagcatttac tatcttaact attttttctc cagtagtttc aacttcaaaa
     9241 gtacaattat ataacttttt ttctcttaca gcttgcataa attcatctgt tactcttaca
     9301 gaaatatttg ctttagtaac cttctccaaa tctgttttta tatctataaa gtcttctatc
     9361 tctggatgat ttacatccat agaaatcata agagctcctc ttctccctct ttgaccaatt
     9421 aagccagttg tcatactgta aagttccata aaagagactg ctccagtcgt atgttttgca
     9481 gcattgttaa ctttcgctcc ttttggtctt agctttgaaa tatcaattcc aactcctcca
     9541 ccataactaa aagttctagc aagttttttt gcagtatcaa atatatcttc tatgctatct
     9601 tttggtggtt ctattacata acaattagaa tatgttactt tttttccttc tttctgtaat
     9661 cctctatttg ctaaaattct tccagcaaat agaaatcttt tttgtcttat tagttttgat
     9721 attcttgaac tattatttga aattctcttt atccattcat caaaactctc accattatgt
     9781 ctatacttgt tcttccatat tgatttttgt aattcatcaa actcccaagc tttagtcctt
     9841 atttcagagc gtctttctct atataatatg tagtgttttg ctatatcttt tctatttgat
     9901 tccataagta gtacttcaac tatatcttgt atttcttcta cggataagca tttttccata
     9961 gaattatagt cattagctat ttttcttgat atatcatatg ccaactctct atctactcca
    10021 ccttcactat ttatactagc tttatagaca gcatcttcaa tcttactttt actaaaaagt
    10081 tcttttgtac catctctttt tattatgtat ttcattttta aacctccaat aaattagtat
    10141 taaattaata cacacattaa ctataactct aaacaaactt tttatatatt tgtatatttt
    10201 catattaatt atctaacata atatatatta ta
//
LOCUS       DAMJWU010000118        10184 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMJWU010000118 DAMJWU010000000
VERSION     DAMJWU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10184
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.123"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(100..1980)
                     /locus_tag="PC460_003184"
     CDS             complement(100..1980)
                     /locus_tag="PC460_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF2602046.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2184..8147)
                     /locus_tag="PC460_003185"
     CDS             complement(2184..8147)
                     /locus_tag="PC460_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HDF2602047.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8271..8356)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8528..9931)
                     /locus_tag="PC460_003186"
     CDS             complement(8528..9931)
                     /locus_tag="PC460_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF2602048.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         2798 a         1881 c         1159 g         4346 t
ORIGIN      
        1 atattattag attgttaaga taatgaaaat aaaataagaa tgcatttaaa tatataactt
       61 tatatctaaa tacattctta ttttctattt acttaatatt tatttttcaa ttaaactctt
      121 aatcttgtct attacagatg aggctattcc tccacctact tcaaacaact ttgatgaatt
      181 tttacctttt aatgacgatt cttgtgattt atctaacgta tttgtagcaa gtataactgg
      241 tgaattgttt ttagctgcta atggtccaac tgctaaagca tctataagtt tatcttgatt
      301 tgcaactaca actccatcta gcttagattg agagtagaat ttatctatta ctttggcatt
      361 cgtctcttgt ctagtttgac ctgccaccct attgtttgat atatctttgt ttgctatttt
      421 tccaacttgt tcaatagcct tgttagatat tttttctact ccaccaataa aatatatatt
      481 tgacacctta tttgatttaa taaattgttc agcttcatta tctacaccat ctttttgtgt
      541 aagtactatt ggagtttttt ctttaccagc cagtgatgct attgataatg aatcagcttc
      601 tccatttcct gctcctatat acaacttgct tacattattc gtcttatcta tttcttttgc
      661 tatattaata gaagtttgat atcttgtatc tccacctatt ctttgtactg atatttttga
      721 atttacagct ttgatagctt caacaactga atttggcatt gagttgtttc cacctatcac
      781 aattactttt gatggattta gccttttcaa ttcttctaca gttttttgag gaagcttttc
      841 atttgatgat aaaagaattg gtgagttttt tacactagca agtggtgtag ctgtaagccc
      901 atccaccata cttttatctt ctccatttac tattacaact gtatctgatt ttgtccaacc
      961 actttgagat attttgatag ctgtctcaaa tctatcagca ccttttaatt ttttgactgt
     1021 gacattagat gtagaatcac ttgaacttcc tcctccagag cttccaccac tagatgagcc
     1081 gcctgaatta cttcctccac cattatttcc accattgcca ttatctggtg gtaattcatc
     1141 cggttttgat aattcatttt caactatgta ttctaaactt tttggaagtg taacttcaac
     1201 atatttgttt attgtagctt ttcttccctt agttgttttt tgtgctaatc caagttttaa
     1261 tattacattt gaaccacttt cttgtgtttc tttactaaca atagaatagt ttatcctaat
     1321 attgtttaat aaatattcaa gtgcttcact tttctctacc ttatactttt cagggtcatt
     1381 tttactagaa ccagttccac caattggttt tacttcactc tcattaataa gggtaaatat
     1441 taatccttca tcagagctta tattttcata atccactgtt ttgttgttat atgttacaat
     1501 ttttaaagct ggtttatcat ttgttccttt tcctaaaact ttatagtcag catttgagtt
     1561 actgccagat ttttttacag atattcttcc tatattcaat ctatttccgt ctagtaaatc
     1621 ttcatttgat gtaacatata tatctaatgt agttgtatta cttgatttgt tgtattctac
     1681 tctacaagta gcatttgcat ttccactttt tatctgagat tttatattat cagaccaagt
     1741 aatcttttct ttctgctcat tatctactac ttcaacctca ccttgtactt ttatactggc
     1801 ttgaaatgaa tatactatgt tttctactga gtccacagat atatctatag tatttacttt
     1861 ctcatacgtt tttgttcctt gtaaaactgg agctgcatat gcacatgtag cactaccagt
     1921 tacagctact gccatagttg atactgctat agttcttata aaagattttc ttttactcat
     1981 taacacccgt ccttatctat cattaactaa gtggataaat ttacttatcc acctagcttt
     2041 tatatttaat ttataaatta ccctaaattt atggttacat ttatatactt tacttttaat
     2101 atctactatt ttaaaactat tctagtacct aatcttatta gatatacaat gttagtattt
     2161 ttacgttttt agagttccac ctactattta tctctaacaa tattaccctt tactttattg
     2221 cttacagtta taaccttctt attactgata tcaatagaat ttcttgtagc ttttctagct
     2281 ttactattac tatcagtaag acttattgtc tttaactcat cataattctt agctcctaca
     2341 ggtacataca ttgtatcact tacaaatctt tgacctactg gagtatctcc ttgaaggtca
     2401 ttgtatctat atagttcagc cttaacctta tcacttaaag aatcaaagtt ttcttttggc
     2461 atatagtaaa cccatgtacc tttactgatt tcacctagtt cacctttttc tggaacttct
     2521 gcaaataaaa gtgcttctcc agatatagtc ttattgtttt tatctataag tagtggtaaa
     2581 ttaaatgctg gatttccact gtattcacct gtaataacta ttgaacccgc tggaatctta
     2641 cctgtttcat cttcacttgt gctagagtca tcactttgat atacataatc ttctgctaat
     2701 tttccaattc cattagtacg agtttcgtct gcattattta ttatgccaat ttctatatta
     2761 tcacctggag gtcctattat atctaattct gcaacttcta tattagactt attatttgag
     2821 attagtttta catatctagc ttggtatgtc caaagtttac catctttatt aaatgtaact
     2881 ctagcttcat tatcttggtc ttttcctcca ccaactaaat cttgtccata ttcaaaagtt
     2941 ccttgactta catcttccca gttcttttta tcattactta cttgtatctt gtaattagtt
     3001 aaaggattgt atgttgtgct agtattcttt ctaaagttaa ataatccttt taatgagaac
     3061 tttctagctt ctgtctctgg ttttgtatat tttagtccta ctataggtaa tgtttctttc
     3121 atgtctacta ttatatatgg attttcttct acatttatat cagggtcttt ttgagggtct
     3181 ttgttccact ctgctttact tattatcttt cctttatatg tagtacttgc atcattatct
     3241 tttatattat caatttcagg attttgtaat ggtccatgtg agtcattttc agttctaaca
     3301 tcaccgtcag aaacagtatt tgttatgaat gaccatgctg atttatttat tttcccttca
     3361 tctgataatt ttacagtacc aacttcaaat tcttctgttg gatttaggtg ataatcatat
     3421 gcaactacct tatatgtaaa tgctctgtta ttaagaggag atatattatc agtaaattta
     3481 gtcttgctat ttccttttgc atccacaaat ccaactgttc tgtattttac ctgtgttcca
     3541 ttttcaccat ctaaataatt tccatcactt cttattattt cgtatcctag tatcttatct
     3601 ttatccttat ctacatctag gtctatagat attttattag atttaactag agaaccactt
     3661 tgtattccat cagaaaaact agcacttact ttagtatctt ttgccatact agctatattt
     3721 tcatttaaaa tctttcttct agcttcatca tttacatatt gtatctttct tgattctttt
     3781 tcgtagttct tgctatccat tatactgttt aattctggtg taatttctac tccccatgcc
     3841 ttaaagtaat ctcttaaatc tttttcaaca acacttgatg ccattgctac tagcatttgg
     3901 tcttttggta aactattaat tgggtcacta cttgaaagtg ctctgtattt tcttgaaatc
     3961 tttgcataga atgtatcatt acttaaatca gcatctgagt tattctttaa cattgaaccc
     4021 gtaaaatcat catcataagc tagatgtaat tgccataaca ttccaagcct ttcaaataca
     4081 ttgcttggta tacctaagct atttgaagtt acatgctcat atatcttatc cattgttgaa
     4141 ccttcaagtc ttgaatgtgt cttatcatca aaggtctgag caagtaaagc aagtacattg
     4201 ttactagttt cgctatatgt catctttcca atatctgtaa catgtcctat ctcatgacct
     4261 attccccatc caaataagtt tcctcctgta gcttttccac tatcatctat ttggaaaggt
     4321 actccattca taagccctgg aactgaacca tattctactc ctacatgtaa tccagaagca
     4381 tacataaatg ctccaataaa cattctttgg tattttatgt tcatacgaga ttttggagcc
     4441 atatcatttt tagttaagtt aaattcatta tcatctacag ttccattacc atctaaatct
     4501 tgtgttttaa atactcctct ttctgagtaa actaaatcca ttatttgttc ccatgcaagc
     4561 atactattat ataatctatc tacttgtgta tttaaatcag aatttccttt tgatatctct
     4621 tgatatactg ctgttgcagg aacatttaat gttactttat cactttcaat atctgtagtg
     4681 tttaacacac cagttttttc tgaatatcca tatgaattac caacatttgt tcctggataa
     4741 tatggttctc cttttacagc atctttatat ttagctaatt cttctatata tgcttttatc
     4801 tttttcttag attcagaaac tcccgcagga ctcttatcat ttattgttcc atttagatta
     4861 agatgtggta tcttagtagc tcctgctaca cgtactttta tatctcctcc acttggagta
     4921 tcttcagcgt atcttatgta taaacttcct ccacattcta tatctaaact atgaagtttg
     4981 cttaattgaa ttacattttt tccagtgtta agatttattt ctccactctt atatgcacca
     5041 gattctccat agtactgtgt atatatcaac ttaggagttt ttccactttt acttccaaca
     5101 tatactgtga tttcatctcc tgctctagca gctactccta atgcctgcca gtcattttgc
     5161 atacctaaat tattggaatt tacagtatac tgtccgtttt tatctttata ttttcctaat
     5221 ttatttattt cttgatttac agtagttaca tcagtaagtg tggctctatc attatataag
     5281 tcttgagcta attttaattc tctctcaata actgtcttat atggatgata ttctccacta
     5341 gctttatctg gagtatttaa tctatctttt aattcagtta ttttttcttg agttacatct
     5401 tcttttaatt caacttgtag gccatccata aatagatttc ttacatcatc ttctatactg
     5461 tcatactcat aaaacttaag ttctgatata ttagacttat atgaatagga tgaacaaaca
     5521 cttacttgta tcttggtagc agttattggt tcttttaatc taaccattac atacttatta
     5581 ttatttacag ggtctgtctt tgtttcaaaa ggaacatccc ccagattgtg taattgacca
     5641 ttttcatccc aatatcgtac ctcaaagtca tttattgtct tatcagaaac atcaaaacga
     5701 ggtgtagcca taaaagtatc catagtataa gatttatcaa aagttattat tggtcctcta
     5761 ttcgttccct cagctgcatt atatgttggg aatgtccaat gtgttgtaaa gtccccatct
     5821 actacattga atttatcaaa tggattttta tcactatatt cacttttgtc atatccatct
     5881 ggataaacaa catctacaat gtgatttgtt ggtattccaa atccaccatt ttctttagga
     5941 gtattaatta atctatagtt aggtgttgtt ggtgcatcca tgctttcagt tgtacctata
     6001 tatgttttag acattccact agttcccaag tggttagtag ctgtcattct gacttcatat
     6061 gttgtattat ccttaagacc ttctatcttt gaacttgtcc cacgaataag cttatcattg
     6121 tattttatcc aagacccatt agagcctttt tctttatagt acaagtcaaa atctttagct
     6181 tgtttatgat ttttccagct tacatcaaga gatttaaatt tacctttaac atttatacct
     6241 tctggtggtt ctggttttgt gtctggaaca acttgaaatt ctaatatact atctgggcta
     6301 aattgtatag gtttatagtt tgaatctaca ttttctggta ctccgtctaa gttttctggt
     6361 gttgcatctt tataaccact agaccaatct ccatttaaag attgtatgct aactctatat
     6421 agtccatatg cttctatatc tctaacttct attgtatttt tactagtttg taatttttta
     6481 gtagataata cttttccctt actatcaatt ttgtctagta taagttcgta tgctgataca
     6541 tttacttcat tattccaata aaaagatact ttttcatttc caacagcagt gctagtcgat
     6601 attttattta ttttaggtat gttcatctct ggctttggta tttctttata tacattgttt
     6661 aaaaattcaa cttttgatat ttctgcaaga tttctatcgt ttgctgttgt agctgttatt
     6721 tttatagtta tcttttttac tgctacttgt ttaccaagat ttattactat atcactattt
     6781 tgaacatctg catatgtttc taatgctgac ctactatatg tagatgttgt agagttatca
     6841 tctttactga tattaccctc ttcttttgga gtatcttcat ctgaattagg tttatccatt
     6901 gatgaatttt ctttatcatt atttttatct tgattggtat tagagtttgc ttcttgttta
     6961 tcgctatttt cctgttgttt atcattattt tcatctttat tatttgagtt attatcagtc
     7021 tggtttatat tttcatcttt attatttact tcttcttgat ttacttcttc ttgttcgtta
     7081 tttacttcct cttgttgctc ctcatctttt aagtttttca tatattcttt gttttcataa
     7141 ggaatctgtt gaacattacc atctttgtca gtaacctcaa ctattccttt tgctggagca
     7201 gtaccttctg gagcttttat attgatgact tccatattaa gtgcttctga taaagctata
     7261 tccattactg ctggtttttc ctcagatatt tcatcatttt ttgaagatat tgtaactcca
     7321 ttattttctc cactaaataa attttctaca ttaccattaa tatctacatc actatttcct
     7381 tttaattcaa ccttacttgt atctactata ggattggtag atactatact ttctaggctt
     7441 tttgcatctt cactttgtcc taaatttttc tgtacataat ctaagtcaac aatatctacc
     7501 tttccatctc tatttaaatc aaattctctt ttatttgaat ctatattctc taaaacctta
     7561 ttataatcac tcttatctac tttaccgtct ccatttacat cacccattaa gaacaattca
     7621 taatttaaag gtttattttt attatctaca cttgctctat tgcttaataa gactctttgt
     7681 gaataacttg atatatcaat gtctttaatt tctttgttct taaatccatc tccagagact
     7741 tctacattgt attttccaac aggtaaatta ttaaatacaa tattataata tgatatattc
     7801 tcatctttat tttttatttc acttctactt gcagataatt tttttatagt atattctata
     7861 ttatctgtcc caacatttac tgttcctgaa tctttttctc caagctttat tattccacta
     7921 ttgttactat catcttttaa tcgtaactgc atattagtca tactttctga ctcgctattt
     7981 tttattggca gtgaaaaatt taaatctact tcaagactcc cccttaactt ttctgtaact
     8041 gatttttcaa ctgcccttag cttcccctct aatataggtt ctgcaaaaac gtttagtggt
     8101 gcactacaca tagatactgc tagtacacaa gatattgcct ttttcatgaa ataacaatcc
     8161 tcccttaata aatatctaat ttttcatgaa tttaaatact ataagtttca gacaataaaa
     8221 aaagacactc aaagagaatc ttaattataa aataaaaaga ctttactctt tttagcaact
     8281 ggctgatatg gctttaaaag ctttaatccc tatagttttg cgtcattaga tttccctaat
     8341 tttgccattt aattttcact actttcttac ccattttata ctatatttaa actttttaat
     8401 ataatttttt tagattaaat tataaaaaaa taacatttat attaatttat taatataaat
     8461 gctattttcc attataattt aattatttat atagcttcct tacaagcata attttgtgta
     8521 tattttctta ttctttatat cttccataaa attcatgtgt agcatataga ttcttatctc
     8581 ctgttatagc tataccaaat cccattattt catacccttt atgcaacata ttagttcctt
     8641 cttctggatt tgtcttccac tcattaaaaa taaagtttcc tatatcatct gcatctctat
     8701 ttgtatatac aggtttatct ggaattttat atttaattat attttcacct tgaactgcta
     8761 tatatccagg tttaatctca ctccaatcta agtatttcaa aaaagtagaa tatgaatttt
     8821 ttccatttat cgtatgagat aaagtcttct tatttgccat taaattagac caatcatttg
     8881 ctagaccctc taatctgctt gctacagata acgctcttac tccattctct tttctatact
     8941 gctttgtaag agcatatact ttacccttta ttctctcttt aaatgctcta tcatttggat
     9001 tgtaatataa accttgttga tttggtgaag cttgatttat acattgcttg ataacattct
     9061 gatttatgcc tcctagtaca gtcaatttgt ctgcaccact taaaatactc ttgttactgc
     9121 cattttcaac taaaactaca ggagcatttt ttgctattgt agaggcagcc aaagcgtctg
     9181 ttaattgtaa gcctttactt agataaaatt ctttactatt tttataaaac tcttgtataa
     9241 ctatcttatt agtttcaaat ctatctgtgc cacccaatct aatagaattt gtgctcttaa
     9301 ctaattcgtc gcttattatt tcactagaac caagcgcata actctttaag cctgtcgtgt
     9361 caaatcccac actatttcca tctgtaagta ttataggtac tccatctcta gcagctactg
     9421 gtgatgcact tatcgcatct gcttctcctg taaatccatt ggctataatt actttatcta
     9481 ttggcttaat ttctttgatt ttttcagcta tttttatact tgtatcaaat ctattttcac
     9541 caaatattct ttgtacaacc ttctttttgg atagtaactg attctcaaca tttttactta
     9601 ttgttgattc tgaacctatt atatacacag tctttgcttt gtttaatctt tccatagttg
     9661 tgtttggaat ctggttttgt tttgtcatca aaataggcgc atttaaggct ccagctaaac
     9721 cacttgcact tagaccatct gctaagctat tatctgagtt gattagtatt gctgtattgt
     9781 agttttgctt atctgctatt tttgctgctg tctcatatct gtcattccct tttatttgct
     9841 caactgatgt caacgcattt accataggag tatttaccat tactaaaaat agtgtaagtc
     9901 ctatagctaa tatcttttta ctattcttca tattattttc tacccccaat gtacttttca
     9961 tattttttat tagtactcat acttattttt aatcaaaagt tacaattttg ttaccatatt
    10021 ttatatatga taccatagtt atccatacaa ctcaaacaat atacctaaag tttttattct
    10081 ctataaattc aaaacattat aatttatttt atttaattac gttataagtt caaaataatt
    10141 tatgttagta atatgtttaa tcattataag aagtatcttt ttat
//
LOCUS       DAMJWU010000119        10056 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMJWU010000119 DAMJWU010000000
VERSION     DAMJWU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.124"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            210..1091
                     /locus_tag="PC460_003187"
     CDS             210..1091
                     /locus_tag="PC460_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2602049.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKISNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            1282..1449
                     /locus_tag="PC460_003188"
                     /pseudo
     CDS             1282..1449
                     /locus_tag="PC460_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(1709..4276)
                     /locus_tag="PC460_003189"
     CDS             complement(1709..4276)
                     /locus_tag="PC460_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2602050.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSNP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRAIKSGQMITVDGLAGTVTFDE"
     gene            complement(4279..4884)
                     /locus_tag="PC460_003190"
     CDS             complement(4279..4884)
                     /locus_tag="PC460_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2602051.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(5258..6718)
                     /locus_tag="PC460_003191"
     CDS             complement(5258..6718)
                     /locus_tag="PC460_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2602052.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            7072..7650
                     /locus_tag="PC460_003192"
     CDS             7072..7650
                     /locus_tag="PC460_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2602053.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYSNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            7701..7934
                     /locus_tag="PC460_003193"
     CDS             7701..7934
                     /locus_tag="PC460_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602054.1"
                     /translation="MIELIQRLNDIIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKFLIIIAIISIIINILIILDR"
     gene            7943..9193
                     /locus_tag="PC460_003194"
     CDS             7943..9193
                     /locus_tag="PC460_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2602055.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9354..9923
                     /locus_tag="PC460_003195"
     CDS             9354..9923
                     /locus_tag="PC460_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2602056.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3348 a         1730 c         1493 g         3485 t
ORIGIN      
        1 tatttaatta tttaattttt attatttgta cttatttaaa gtttcatcta attataaatt
       61 ggacaatttt tatgttgatt ttattcatac aaattaaagt aacttataaa atctccacaa
      121 taaaaaacat tttttattca ctaaactgct ccatcattgg tttagcaata aatatatttt
      181 ttaggtcttt aatgtcaata gtgttaaata tgccagcata tttatttgat agtaattcta
      241 ttgcaaatat ttacagagta tttcctatat catttagctt tatagttaca ggactatatt
      301 tcagttataa aaataataaa aataataaga ttaactttga ggcagtttta gaggacaaaa
      361 aaaacttaat tttcttattg agccttatgt ttggaatgta cttgtatcta tgtttgagtt
      421 tgtcaatata ctatactgtt ggaaatgtat tgattttaaa attatatgga atcaagtctg
      481 ccataatagt tttggtaggt aactatattg cagaaagata tagctttaga attactcaaa
      541 taaaaggtta tgatattaaa aatcaaatag aatatgtaga aagtgtaaag acagagtatg
      601 agattaataa attgaatgat attgtatata cagatttact gacaggtttc tacaatagac
      661 cttttgtgaa tcaaaaacta tcagaatgga catcaaatca ctacaaattt accttatgtt
      721 ttactgattt aaatagctta aaacctgtaa atgataaatt tggacaccaa tttggtgata
      781 aatacatatc cactactgct caaataataa aaaaacttat aaatgtagaa aattccttac
      841 tatttagata tggtggagat gaatttttat tccttttaca gaatacaagt tgtgtagaag
      901 cagaaaagat aatgattaat ataaattatg aattatcaaa aataagtaat accgacgagt
      961 atccttatgc tctttccata agctatggat tagttgagtg ggatggaata tccgatattg
     1021 aaactttaat atcaaaggca gattctttga tgtatgaaaa taagcttaaa aataaatcta
     1081 ctttaaacta gctagttcag atattctaca aaatatatat tataagatag tactaagaat
     1141 gtcaaaatat atagattttg caaatattga atacagttta ctaatgaatg cactggatgt
     1201 aagtgttagt aaacacctat tagatgaaca ctttactgtt gtttgggcta atgaccgtta
     1261 ttatgaaatg ttcggctata cctagctaaa aagttaaaaa tgaccactgt agccgaggga
     1321 atagagacta gttttcaaaa agatttcttg caagaaatag actgtgatat gttacaagga
     1381 tacgtgttct ctagaccttt accaataaaa gactttgaaa aacttatgtt tcaaaactcc
     1441 tccaattaac aatataaaat tgcttattat aagtgatatt attgtatcaa caaataaagg
     1501 aattgtctca aaattgattt tttctaatca aatttttatt ttgagacatt ctcatttttt
     1561 tgcataagaa aatacccacc aagctacggt ttttcttacc gctgttcggt gggtattagt
     1621 agtattatga aatctgaaaa ctttaagtat tactacttcc gctagcgcaa tcacaaaggt
     1681 cgaaagcttc aaagcgaaaa taggcttttt actcatcaaa cgtcaccgta cctgccaacc
     1741 cgtccaccgt tatcatttgt ccgctcttaa tagctcgtgt ggcaatgtgt gtagccataa
     1801 cggctgggat gccatattca cgtgcgacaa tgcttgaatg gctaagagga ccaccaatgt
     1861 ccgttacaat cgcacttgca gatgaaaata atggtgtcca tgctggagtt gtggtaactg
     1921 caaccaaaac acttccaggt tgaaagcttt caaaatctgc tggactattc agtacacaag
     1981 ctggagccgt cacaacacca gtactggtac caatgcccgt caacacaatt ttcccatctt
     2041 tctgcttctg ttgtgcttgt gatatagttt ttttgttttt ctctggcaac ttggatggtg
     2101 acacataacc catgtatttt ttaagttcag cttttcgaac aggtattatc ccgttcatat
     2161 cagatagaga cttgttttta tccaactgct ctataagtac ctccaattcc gattttgtga
     2221 gccaataaat atcatctaaa tgtgaaatag ctcctcctcg tatgaatcgt tctgagattt
     2281 cctgaaacat acaacgaatc agaggatgtc ccattcccat caaataaata gcattttcac
     2341 gcataggaga ggtttcctgt gcccaatgga gcaacttaaa aaatagtttt ttacgcaatc
     2401 cacctatgtg ttgtaaaatc tcttcttctg catgttttct acgcttttca aacgtggtct
     2461 ggcgtaaaaa aggactttcc ccttttcctt ccacaaatgt ttttacggat tcaaaagttg
     2521 gtgtaagtgt ttcctgcggt gtggaatgcg caaaatcaaa ctcataagcg gtacgaccaa
     2581 actctttaaa gtacttattg attcgatttt tccattctat ccatacttcc tgtgaaactc
     2641 caacaggcac aactgaagac atgaaatctt ctgctatctt tattgcagga ctgttttgca
     2701 gataaaggtt gatgctgtta ttttgcttta cccattctga aataccccac aactcctttt
     2761 ccgattgcag aacaatcgta tcaaacccaa gcaggaaaac ggaagtatcc gttataccag
     2821 cacgtcgaac tgctccttga aataattttg taaataatgt ttcactgata gaagcagcag
     2881 gcaatgtaag ctgaatcctt gtgaaataaa tacatgcggc ataaaatatt gtctgaatac
     2941 cctccataat ttgatgggcg ttcatagtat gcaggggctt ttcttcccat tgtttaatag
     3001 catcctcata tccttttcgt gccgtttccc agcgtgcaac gctgttcata agtgtcctac
     3061 gtaaagagcg tggggaaagg gatttgatag caatcaaaag tggatttgat ttagcagaaa
     3121 gataaacata tccattaata accgtatatg ccccgttttt tggtaataat tttttcgcac
     3181 ttttaccaaa catatctatc cataaaagct ctgatgcacg gtttacgatt tcaagaccaa
     3241 gcgtggcgaa caaaggtgta acaggatttg gcaaatgttc tgccaaactt ccctttgtgt
     3301 atacaacatc ctgttctggc aatacccatt ctggtggcag aacagtaatt ggacgagcct
     3361 gaacaatgta caatttatct ttttccagtg cccactctac atccataggc atttgataat
     3421 acttctcaat tttctttcca agctgtatca gttgcattac ctgattacga gtaagagcat
     3481 atttttttct caacctttca ggaaccatgg tttcttctgt tccttctgaa gtacgaaccg
     3541 tcataatctc tttatttgcc acttcatacg atataattcg ttcggagtcc ttatccacta
     3601 cgattgtatc gggtgtaact aagctagaaa ccaccgcttc accaagcccc catgcagcgt
     3661 taataatcat ttcgctacgt ctaccattga ttgggtttaa cgtaaacata ataccagatg
     3721 aatcggaaaa tgcaagcttt tgtactacaa cagcaagtgc gacaatctcg tgctttatat
     3781 tattcttcac acgataagca atagcacgag ctgtccataa ggaagcccag catctcttta
     3841 cagagtcaat aactttatct tcaccttgta tgttaagata agtttcctgc tgacctgcaa
     3901 aagaagcgtc aggtaaatct tcagcagtcg cagacgaacg gacagccacc gatatgttcc
     3961 ctaatccagc ataagccatt ttaattgcgt ccgacacttc ctgtggcatt tctccattat
     4021 ggaaaagctc ccctattttc ttagatacat cttcaagttg gctggtatta ttggaatcaa
     4081 taccatctag cagtttatta attcgagact gaatatggtt cgtctcaaca aaaatctggt
     4141 atgaagcagt cgtaacatga aaaccgtctg gcacagggat gcctgctgtc aatagttttg
     4201 ataaagacat cccttttcca ccaacaattt caatggttgc ttgtttatgt tccagtggca
     4261 agacataata aatcataatc attcacctcc atcattcata attccgtgca gaaatatatt
     4321 cattgtttca tccaacattt tttccgaact atccaatggt cgtgacgtgt agctgattga
     4381 taacatggag ttaattaaca aacgtgtcac aagagcggta tccgttttgc gaaagatgcc
     4441 tgtggtcaca ccattatcaa ttaccgattt tataatatct tgatatgcat aacgagcatc
     4501 tttaagtctc tttcgatact cttcttctaa ataatcagat tctgtattta accacatcag
     4561 tacagttcta tactgctctg gaaatctaag atgattaagc atgatttttc taaggcactg
     4621 ttgtggtgaa ggttcaccat caataatcct atgaacttgt tggatagttt tttctatttc
     4681 tttctcaact gcatatacaa caatctcgtc tttcgttttg aaaaaatcgt atagacttga
     4741 ctttcccatg ttggctaaaa ctgcaatttc tctcattgat gttttttgga atccattttc
     4801 agccataaga ctcaaagcaa cagcaactat tttttctcgg cgttctgctt gttgttcggg
     4861 tgtaagcaca attccttttg gcatagggcg atacctcctt ttagcgacca ctggtcgttt
     4921 cttttttatt tatcatacag cgaccaacgg tcacttgtca aggttaattt ttttcattgt
     4981 atatggtact cgagataaaa atgttcattt tagaaattgc tctatttaag tagaaatagg
     5041 cttcatcatt agaaaacaag actataaatt tgagatattt atcatgttca aatttcatca
     5101 atatcaaagt ttaagatatt aataaaaaaa gaattactaa gaaataacat ataaagattc
     5161 aaaaaaggag ttgaacttaa aattcaatta caaaaaatca aatttaagac aactccttta
     5221 ttctttatct ctactattct ttatctctat ttatttatta caactcccta acatttacca
     5281 ctactgcctt aggtctagtc atagcctcga ttgaatactt aacaccttga gttcccatac
     5341 cagatgcctt tacacctaaa aatgggaagt ggtctggacc tctttcagtc ttattattta
     5401 tttgaacagt accaacttca agtttatcag ctatataaaa tgccttatca atatcacttg
     5461 taaatactga tgattgaagc ccatactctg attgattagc aatttcaatt gcttgattta
     5521 tatctttaac tcttataatt ggaagtactg gaccaaatgg ttcttcccaa gcaattttca
     5581 tatcaacatt tacattatct aacaatgttg gatagaataa attatttttt ctaacattgc
     5641 ctgttattaa ctttgctcct ttatgtaatg catcatcaac caacccttga acaaaatcag
     5701 ttgctttatt atctataaga ggagtgataa ctacttcatc cattggattt ccaattgtca
     5761 acttctctac caatggcttt atttttccga ctaatttatc cgccacactc tcttgtacta
     5821 aaattctctt tactgccgta catctttgtc ctgaatatga atacgctcca gatactatat
     5881 tcttagctgc aaaatctaag tctgcatctt ctaatactat agccgcatct tttccaccaa
     5941 gctccaataa taatggtacc attccagata ttttagatat atgctgacca acttcagtac
     6001 taccagtaaa gttaataaaa ttaattcctt tatgcgttac aacataatct cctatctcac
     6061 tacccctacc tgtaatagta ttaagtactc catctggtat cccagctttt ctcataattt
     6121 ctaccacatg aagtgcgcta atagcccctt gtgtagcagg ttttaataca actgcatttc
     6181 caccaataag tgctggagca atctttgaca ttgataaatt tacaggataa ttaaaaggag
     6241 atatagctaa aacagtacct aatggaaccc ttgaaacata agacatctta tttcttgttc
     6301 caccagggaa attatctcca ctaatggctt caccctccat attttttccc acatcagctg
     6361 tgtatcttaa aaaatctgct gttctctcaa cttctgccct tgcagatttt atgtctttgg
     6421 ctatttcaag taccagtata ttagctatct catctatatt ttctaacaat aaatcagcag
     6481 ccttatgaaa tatattggct ctcttatata caggcatttc agcccatact tttatactct
     6541 cttttgtgtt ttttataacc tcatctacct catgttttgt catagattgt actttcccaa
     6601 caagagaatt atctattggt gagtaaattt ctattggctt tctcgaatta gattctaccc
     6661 attttccatt tattaaattt ttatatacat tttcttttgt tttaagttca ttaaacatat
     6721 ttttattcct ctctctgata taactttgtg tatttaaatt atagccattt attcgttatg
     6781 taaacatttc tttttttcat aaacgtttac ctataaaaaa attttaagtt aacgtttttc
     6841 tacattcttc tattttaata acatactcgc tccaaatcta tataggtcac actttaatat
     6901 caatatacta atataccaat ataccaatat accaatatac caatatacca atataaatat
     6961 tataaacata aatcacttga caaatttata agtagacaat attatatatc tatgacttaa
     7021 aatattttta aagtcataga tatcatatat agatagaaag gattgataaa aatggctaaa
     7081 agttttacag aaatagaaaa agacaatata agaaaaagac taatttcaga atgtgaaatc
     7141 aactggtcta aatatggata caaaaaaact aatattgatg aactttgttc aaaatcagga
     7201 attgcaaagg gttcattcta cacattcttt gattcaaaag aaaagttatt ttttgaagta
     7261 ttaaatggaa tccaaaacag acttatatca tcagtagatg aaattttaag taacatgcct
     7321 ccaaaagaag gctttgcaaa agccttaaag atgttgtatc gattatatga taaaaaccca
     7381 ttcttatatt caccaaataa tactgattac atcactttat tgaataagat acctaaagag
     7441 atgctttcac aacttgaata cagcaatgta caaagttttt atagcttaat tgaaagacat
     7501 aatctcaagt taaaaataga gaaagaaaaa gcctttggtg tttgcaatgc cctactgtca
     7561 agcctattgc taaaagaaga tattggatat gactacattg aagtatttga ttttatggta
     7621 gacaatcttg tagaacacat acttgagtaa taatttaaaa taaattttct aaacatttct
     7681 ttataactag gaggagtaat atgattgaat taatacaaag actaaatgat attatttaca
     7741 aaataaatga taaactaaat atccacacaa aacttgtaga ccctagcagt gagctcatgc
     7801 cactttactc tatgttatct ataatcgttt ctatatttct aataatatta ggtattaaag
     7861 taagaaaaaa attccttata ataattgcta ttatatcaat tattataaat attttaataa
     7921 tcttagatag gtaggtacat atatggtaaa atcctcgaac acttttaata aaaacttcaa
     7981 cttgatgata ttaggtcaaa taatctcatt gtttggtgca tcaatactta aatttgcttt
     8041 atctttatac atacttgata taactggtaa agcagaaata tttgctacta tactagcagt
     8101 atcttcaatc ccaattatag tattctcccc tattggtgga gctattgcag atatattcaa
     8161 cagaagaaac ttaatggtta tttttgattt ttcaagtagt cttacagtac taatcttagc
     8221 agtattctta ttcaacaata atggttcaat attacttgta ggaattatta tgaccatatt
     8281 atcagtcata agcacaatgt atcaaccaac tgttcagtca agtacacccc ttttagttga
     8341 taatgagcat ttaatgaatg caaatggtat tgtagctgga gttgcatctc ttacaaatat
     8401 tgcaggacct gtattaggag gtgtcttgta tggtgtgatt ggaattaatg caattatagt
     8461 tataagttgt gtaagtttct ttctatctgc aattatggag atatttatac agattccttt
     8521 tatcaaacaa gagcaaaaca gcaacattat aaaaactata tttactgata taaaggatgg
     8581 tattgttcat atatgtaaag aaaatagatt tatactaaaa atactttttt tagctatagc
     8641 aataaactta tttttaagct ctatgttaat agttggtatc ccctatatag taaaaatcac
     8701 tatgggagct agtaacttct tatatggtct agcagaagga gcaatttctt ttagttcact
     8761 ggtagctgct gttagtattg gtattttctc aaaacatcta aaaatcacta atttgcatat
     8821 ttcctttatt atatgtgcag ttatgttcat accaatggcg gtttcagtat atccatttat
     8881 attaaacaca ggatttccag ttccattttt aatctttata gtatcatcta tgctaatatt
     8941 ttttacaacg actcttatct caatatatgt tataacagtc acacaattaa aaactccaaa
     9001 tgaactctta ggaaaagtaa tgtcaattct ttttgcagga acagcaattg caatgcccct
     9061 tggtcaggtt atgtatggta aactttttga tttattatca gatagagtgt acatcattat
     9121 actaggagtt ggatttatta cattacttat agcatttgta tcaaagataa cattgaggaa
     9181 cgaaaatctt tagatacaaa gaatttagat ataaagattt aattttcact aatttctgaa
     9241 tttaaatgca aaattgtgta tcagtatgtt gacgttttag aaattgattg atataatcaa
     9301 tggaaaataa actttataaa aattcaaaat attcagctag aggagtgata aaaatgagtt
     9361 acatgccaac tttagaacaa gcatgggaaa tattaagaaa atacaataag gaagaatttc
     9421 atataagaca tgctcaaatt gtatctggag ttatgagata ctatgcaaag gagtacgacc
     9481 ctgaaagagt agaattttgg gaaatagttg gacttttaca tgatttagat tttgaaatgt
     9541 atcctgatga acattgtgtt aaacaaagag aattaatgac agagcttgat ttagataaat
     9601 caataatcga ttctacaata agtcatggat atggattaac tggttcagat gttaaaccag
     9661 agcaatttat ggagaaagta ttatttgctg tggatgaact tactggttta attggtgctg
     9721 ctgcaataat gcgtccttca aagagcgttt ctgatttaga attaaaatct gttaagaaga
     9781 aatttaaaga taaatcattt gctgctggct gttctcgaga cgttataaga agtggtgcag
     9841 aaatgcttgg atgggaactt gataagctta tccaaacaac tatagaagct atgagaagct
     9901 tgattccaga gatggcaata tagctcctcc atacatatac catagaagtt acatctttaa
     9961 agagcatttc tctttttgat gcaacttctt tttatatttt gtagaataaa aagtttaata
    10021 ttttaaactc actatataat ctataataaa tactat
//
LOCUS       DAMJWU010000120         9688 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMJWU010000120 DAMJWU010000000
VERSION     DAMJWU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.125"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            111..1397
                     /locus_tag="PC460_003196"
     CDS             111..1397
                     /locus_tag="PC460_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2602057.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1509..1982
                     /locus_tag="PC460_003197"
     CDS             1509..1982
                     /locus_tag="PC460_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1641..1643,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF2602058.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2014..3324
                     /gene="grdB"
                     /locus_tag="PC460_003198"
     CDS             2014..3324
                     /gene="grdB"
                     /locus_tag="PC460_003198"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3061..3063,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2602059.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3580..5112
                     /locus_tag="PC460_003199"
     CDS             3580..5112
                     /locus_tag="PC460_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF2602060.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5123..6268
                     /locus_tag="PC460_003200"
     CDS             5123..6268
                     /locus_tag="PC460_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF2602061.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6578..7657
                     /locus_tag="PC460_003201"
     CDS             6578..7657
                     /locus_tag="PC460_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2602062.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7770..8369
                     /locus_tag="PC460_003202"
     CDS             7770..8369
                     /locus_tag="PC460_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602063.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8556..9428)
                     /locus_tag="PC460_003203"
     CDS             complement(8556..9428)
                     /locus_tag="PC460_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2602064.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
BASE COUNT         3723 a         1120 c         1848 g         2997 t
ORIGIN      
        1 ataaaaacac aataatatat ctttaaaaaa ataagataaa ttgtaaaatg atataatttt
       61 gagaaaaact caaatgcata tacacaaatt aaataacagg aggtgcaagt atgcgtcttg
      121 aattagggaa aatctttata aaagatattc aatttggtga tgtaacagaa gtaaaagatg
      181 gagtattatt catcaataag caagagatgc tacaagaaat tggtggagat gagcatatta
      241 aatctataga tatagaatta gctcgtccag gtgaaagcgt aagaataaca cctgtaaaag
      301 atgttattga gccaagagta aaagttgaag gtaatggggg aatcttccct ggtatcatgt
      361 ctaaagttga cactgtagga gaaggaaaaa ctcatgcttt aaaaggtgta gctgttgtaa
      421 ctactggaaa aatagtaggt ttccaagaag gtataataga catgactgga gaaggagcta
      481 agtacactcc attttctaaa ttaaataatg tagtagtaat agctgaacct attgatggtt
      541 taaaacaata cgctcatgaa aaagctgtta gaatgatagg attcaaagca gcaatgtact
      601 taggagaagt agcaagaaat cttactccag atgaagtttc agtatatgaa actaaacctc
      661 ttcttgaatc aataaaagaa tatccagagc ttccaaaagt aggatatgtt tacatgcttc
      721 agactcaagg tttattacat gatacttatg tatatggagt agatgcaaaa caaatagtac
      781 caactttatt atatccaaca gaattaatgg acggagcaat tgtaagtggt aactgtgttt
      841 ctgcatgtga caaaaaccca agttatgttc atataaacaa tggggttgtt gaagacttat
      901 atgcaagaca tggtaaagat ataaactttg taggtgtaat tataactaat gaaaatgttt
      961 atctagcaga taaagaaaga tcttcaaact ggacttctaa attatgtaaa tatttaggat
     1021 tagatgcagt tatagtttca caagaaggtt ttggaaatcc tgatactgac cttataatga
     1081 actgtaagaa gatagaaatg caaggtgtta aaacagttat agttacagat gaatatgctg
     1141 gacgtgatgg aggttctcaa tcacttgcag atgcagatgt aagagctgat gctgttgtaa
     1201 caggtggaaa tgccaaccaa gttgtagttc ttcctaagtt agataaagtt attggacatt
     1261 tagaagtagt agatgtaata gctggtggtt ctgatggaag tttaagagct gatggaacta
     1321 ttgaagttga aatccaagct ataacaggtg caactaatga aacaggattt ggtcatttaa
     1381 ctgctaaagg atattaagaa gtattttatt aaacgattga gtaatgttaa taaaacatat
     1441 tatagataat tataataagt gttatagata tataaaaata tattagaaat ataataagga
     1501 ggattattat gagtttactt agtaataaaa aggttcttat aataggtgac cgtgatggta
     1561 taccaggacc tgcgatagaa gaatgtgtaa aaacagtaga aggagcagag gttgttttct
     1621 catctacaga atgctttgtc tgaacagctg ctggggctat ggacttagaa aatcaaaaca
     1681 gagttaaaga tgctgctgat aaattcggag ctgaaaatgt tgtgatttta ctaggtgctg
     1741 ctgaagccga agctgcaggt cttgcagccg aaacagtaac tgctggagat ccaactttcg
     1801 ctggaccact tgctggagtt gccttaggat taagtgttta ccacgttgtt gaggaaccaa
     1861 taaaatcatt atttgatgaa agtgtatatg aagaccaaat aagtatgatg gaaatggttt
     1921 tagaagttga agaaatagaa gaagaaatgt ctggtataag agaagaattt tgtaaatttt
     1981 aatgcctaaa agcaaaaggg gtgaaactaa gagatgggaa aactaaaagc agtacattat
     2041 attaatcagt tctttgctgg tataggtgga gaagaaaaag ctgatactaa gccacatgtg
     2101 gcagaaactt tacctccaat aagtttacaa cttgataaat tattaggtga agatattgaa
     2161 atagttggaa cagtagtttg tggagatagc tatttcaatg aaaatataga tagtgcaagt
     2221 gaagaagttt tatcaatggt aaaaggtttt gaaccacaac ttttcatagc tggtcctgca
     2281 tttaatgctg gtagatatgg ggttgctgct ggaacaataa ctaaagttgt aaaagatgct
     2341 ttgaatatac cagctttaac aggtatgtat gttgaaaatc caggagcaga tatgttcaaa
     2401 aaggatgttt atgtagtaga aacttctgat tctgcagcag gtatgagaaa ggcacttcct
     2461 aagatagcta aacttgcagt aaaacttgct aatggagaag aaattggaac tcctaaagat
     2521 gaaggataca tagctagagg aataagagtt aactacttcc atgaagacag aggttctaag
     2581 agagctgttg acatgttagt taagaaaata aaaggtgaac catttgaaac tgaataccca
     2641 atgccaaact ttgatagagt tgacccaagt aaagctgtaa aagatttatc aaaatgtaaa
     2701 atagctttag ttacttctgg tggtatagtt ccaaaaggaa atccagatag aatagagtct
     2761 tcttcagctt ctaaatatgg aacttattct atagcaggag ttatggattt aacagaagag
     2821 acttatgaaa ctgcacatgg tggatatgac ccagtgtatg caaatttaga tgcagataga
     2881 gtattacctg ttgatgttct tagagattta gaaaaagaag gtgtaattgg aaaattacat
     2941 gagactttct atacaactgt aggtaatggt acatctgttg caaactctaa aaaatatgct
     3001 tctgaaattg gagcagcatt agtggctgat ggtgtagatg cagtaatatt aacttctaca
     3061 tgaggtactt gtactcgttg cggtgcaacg atggtaaaag aaatagaaaa aactggactt
     3121 ccagtagttc atatgtgtac agtagtacct atttcattaa cagtaggggc taatagaata
     3181 gttccaacta tagcaatacc tcatccactt ggaaatccag cgcttgaccc tacagaagaa
     3241 aaagccctaa gaagaggcct tgtagaaaaa gcattaaatg ctttaactac agaagtagat
     3301 ggtcaaacag tatttgaaaa ataaaaataa cacacacata tattatatag ataaaaatat
     3361 aaacacacac taaaaataaa aaataactat acacaaaaaa ataatataga tttcatgaat
     3421 ggataaattg gagttattca ttcatgaaat cttaaaaaat gccaaaatac ataaacaata
     3481 atagaatcaa atatacaata aaatacaatg attagagctt ttaataagtt taataagatt
     3541 aaaaaataag tgttcataaa atttatggag gtgcacaaaa tgacttatcc agttttaaaa
     3601 ggggcaggct atgtacttat tcatactcca gatatgatag tgcaaaatgg aagtacttgt
     3661 acagttgaaa gagcaacaaa tccagattca gaatttttaa aagaagtaag taatcatata
     3721 agaagttatg aagatgtagt taactacatg ccaaatcaag tttatatagg aaatagaaga
     3781 ccagaagaat taagagattt accaatgcca tggtgtgaac aaaagattga gggaactaga
     3841 aatggtaagt ttggagaaat aatgcctcaa gatgagttca tagctttaat gcaaataagt
     3901 gatgcatttg atttagtaaa attatcacaa gaatttattg atgaagtaaa gccaaaaatc
     3961 gaaaataact accctgaaat tgcaccattt gtaggtaaat taaagggtga tgatatagaa
     4021 gaaggtaaag aattagtagc aacacacata gctgaaggat tataccatga tggaaaattt
     4081 gttggttatg taaaaagagc acatgatgta gatgttaact taaatgccca tacaatgttt
     4141 gaaaatctag tagtaaaagc atctggagtt ttatctgcta ttcaaatgct aagacatagc
     4201 aaaatagacc ctgctgaaat agattatgtt attgagtgtt cagaagaagc ttgtggagat
     4261 ataaaccaaa gaggtggagg aaactttgct aaatcaatag ctgagatagc aggacttcaa
     4321 aatgcaactg gttctgacac aagaggattt tgtgcagcac caactcatgc attaattcaa
     4381 gcagcagctt tagtaaaagc tggcatacat aaaaatgtaa tggtagtagc aggtggagca
     4441 agtgctaaac ttggaatgaa tgctaaagac catgttaaaa aaggtcttcc tgtacttgaa
     4501 gatgttgttg gaggatttgc agtacttgta tctgaaaatg atggtgtaaa tccagttata
     4561 agaactgatt taacaggtaa gcatactgtt ggaactggtt cttcaccaca agcagttatg
     4621 acagcactta taacttcagg tcttgataga gctaatttaa aaataacaga tgttgatgtg
     4681 tattcagttg aaatgcaaaa cccagatata actaagccag caggagcagg agatgttcca
     4741 gaagcaaact acaaaatgat aggggcactt gctgttaaac gtggagactt agaaaagaaa
     4801 gaattgaaag attttgtttc taacaaagga ttaccaggat gggcaccaac tcaaggacat
     4861 ataccatcag gagctcctta tataggattt ttaatagatg atttaactac aggaaataga
     4921 aatagagcta tgatagttgg aaaaggtagt ttattcttag gaagaatgac taacttattt
     4981 gatggagtat cttttatagc tgaaagaaat acaggtgtta ctgaagagac ttcaggaata
     5041 tctaaggatg aaattaagaa gataatagct gaatctatga aaaaactagc attagatatg
     5101 ttagaagaat aggggtgagg atatgtcaaa aaaagtaatc gcagatgtat tcttagaagt
     5161 agctaatgca atagaaagcg gcgaatttgg caaaaaagta aaaattggtg ttactacact
     5221 tggaagtgaa catggtgtag aaaacatggt taatggtgca caattagcaa aatctaattt
     5281 atttgatatt gtgcttattg gaccaaaagt ggaaactgac cttgaagtag ttgaagttaa
     5341 tgatgaaaaa gaaatgcatg caaaaatgga agaattatta gattcaggat atattgatgc
     5401 ttgtgttact atgcattata attttccaat aggtgtatct acagtaggta gagtaataac
     5461 tccagcaaaa ggaaaagaaa tgatacttgc aacaacaaca ggaacaagtg ctactaatag
     5521 aatagaggca atggtaagaa atgccattta tggtattgct acagctaaat caatgggtaa
     5581 caaatgccca aaagttggaa tacttaatgt tgatggagca agacaagttg agaagtgctt
     5641 aaaagaatta aaagataatg gatatgacat ggagtttgcg gattcaatta gagcagatgg
     5701 tggatgtgta atgagaggta atgatttact tgtaggtgca cctgatgtta tggttactga
     5761 tacattatct ggaaatatat ttatgaaagt attttcttca tatactacag gtggagatta
     5821 tgaagctcaa ggctttggat acggacctgg agttggagag gactatgata gaaaggtact
     5881 tatagtatca agagcatcag gttctccggt agtagcaaat gctttaaaat acgcttatga
     5941 tgttgtaaag ggagatataa gtaatgttgc aagaaatgaa tttgcaaagg ttaaaaaagc
     6001 aaaatttgat gacattatat cctctcttac taaaaaagaa gttaaagctg aaaaagtaga
     6061 agttaaaatg ccagataaag aaattgttac tcgtcaaata gctggtgttg atataatgga
     6121 tttagaagat gcagtatctg agttatggaa aaacggaatc tatgctgaaa gtggtatggg
     6181 ttgtacaggt ccaatagttt tagtaaatga agctaaagga gacttagcag ttgaaacttt
     6241 agttaaagct ggatatactg caaaataagt acatttttta tatgtaaata agaacatata
     6301 gttataattt tattgattat aattatatgt tctttttatt ttatatcttg tcaatattta
     6361 tatattaaca caatccattt atttgattct ctagctaatt tattatattt attcaattaa
     6421 aattaagctt tctaactaaa taaaaactaa tgaagtatta aaatgaaatt gataaaatat
     6481 gaaaaaattt taggaataaa gtatctgtta gataaaaata agattaatta gtgatataat
     6541 ataattaaga taaaatttat tatttggagg taaaatcatg agcagagtaa aaaatgtagt
     6601 agaattgttg gaaactaaag gtgttgatgc actatatcta acaaagaaaa ctaatgtaaa
     6661 ctatataagt ggttttcctg atgaagaagc atatgcagtg atttgcaaag atggaaactt
     6721 tttagttact gatagtagat atatggagct tgcagaaaag gtttgcaaag attttgaaat
     6781 tataaattgg cataattttg atagaagtgt ggcaaaggca gttaaaagtg tatgtgataa
     6841 agtgggtata aagaaacttg gatttgaaag aactaatata gtgtttgata aatatgaaga
     6901 acttaaaaat ctgattgaga aggataatgg agaattaata cctacagaaa atattgtaga
     6961 gactttgaga tatgtaaaag acaaagatga aatcaaaaat acaagaaaag cttgtgaaat
     7021 agcggataag gcattagaag aattgattcc acatataaaa gctggagtta gcgaaattga
     7081 actggctaca aggttagagt actttatgaa aatgaacgga gcacaaaata tagggtttga
     7141 aactatactg atatcaggag caaagacttc attgcttcat ggtaagccta gtgataagat
     7201 tatagaaaaa ggtgattttg tacttataga ctatggtgct atgtataatg gctatatatc
     7261 tgatacaact agaactttta tagttggagg agcttctgaa aagcaattag aaatatataa
     7321 tttagttaag gaagctcaaa atgtaggggt tgagaacatg aaagctggtg tacatgcgac
     7381 aattccagat gctgagataa gaaaagttgt caaaaaatat gaagactatt actatcaagg
     7441 tataggacat ggagtcggta gagatgttca tgaagaacct ttcattggta attatggtga
     7501 taaaatcata gaagagggat gtataataac aatggaacca ggtatttatt tccctggatg
     7561 gggtggagtt agaatagaag atactgtttt gattactaaa aatggtccag aaagacttac
     7621 taaattccct aaagatttga tgattttaga caaataattt aatttatata tattttttta
     7681 cataaaatat aacataatct cactattaag ctctatttac atattatagt aaagagggct
     7741 taattttatt gtgagaggtg atagtatata tgtataaaaa acataaattt ataaaaaata
     7801 taaaaaaagc ctggtataaa atcaaattaa ttgatagatg ccttataatt gtaatggcaa
     7861 tattaatgtt tcaatctata tataacttat ttgttaatga agtaaattca caagatacta
     7921 caactattga tgtagtagta agaaccactt cagcagctat atttgggtat tttttaagtg
     7981 ctaattttat aaagagacct ttaaggaagt atacaaatac tgatgtatct tcaaatcttt
     8041 ttatagataa taatttatcc ccaaaaggtc aagagtctaa tcaaaataat ataatgaatg
     8101 tcaaaaatac cattggattt acttcagaag ctgataatta tgaaaagaaa atacctatta
     8161 ataataatga aggttttgaa gaaggagaaa ctagtgaatt gcaaattatt atagctactg
     8221 taatttgcat agtagcatta acagtacttt ttgttgtaag aaattttaca actacaacat
     8281 cagcttcttt aggtactatt tcacagatga gagattttgt ctcaggatgc gtgggttttt
     8341 tgctaggttg tccaagtaaa cctgattaaa aaagtattat caataaaagg tattttaaaa
     8401 agtatcagta taataatatt tagatttaaa tgtatacaat tataattctg taacattcta
     8461 tattgtattt ttatactgat actctaaact atactattta ttaaaatatt ttaacattgt
     8521 atttaaacaa aatattatat ctgtataata taaatttagt ttatatgaat agatgccata
     8581 tactcaataa aaagtttcat actatgagat atgtattgat ttcttctcca aagaacccca
     8641 atatttgcag taattgcacg ttctaatggt attgaacaga gattttctgt attttgaaat
     8701 atttctttat acaatattgt tgaagccaag tcattttcta gtaaataacg tatggtagaa
     8761 agttgatttg agtgtaaaat tatatttggc ttagcttttt caagtctttt tgtaataatc
     8821 ttatagtgat aagaaccttc agataagatt acaaaaggta cattttttaa catataattc
     8881 gatataatag gatgttttgc caattcattt ttgggatttg taactaagta taattctgtt
     8941 ttatagattg tctttgaatc tatatcatca taaatatcac tgtcaattat accaatacaa
     9001 aaatcaagtg tagattcttc aatcatttta gctccaacaa atgttggaac ttcaaaaatt
     9061 ttaagctcta tatctggata aattacatta aacctaggaa gtatttttga aagcaaaaac
     9121 gtacctaaaa ctgtaggaat acctattttg agagaagctt ttctcttttt tcctaaatca
     9181 agtgcgtcac attgaaaatc ctcaaattgt tttataaaat gttttgactt gtctaaaaaa
     9241 atttttcctt caggcgttaa ctctatttta ttacctattc tgtaaaaaag ttcaaagcca
     9301 agttcttttt ctaattcttt aattgctaat gttatggatg gttgagaaat atgaatactt
     9361 tccgcagcct tagtcatgtt tttatattta cagacagttt gaaaatagta taaatagttt
     9421 atattcattt ggtattagtt cctttcattt acatattagt gacaagtata taattaacaa
     9481 tagcataagt tttgtatatt gttgtaaaaa aatagaatag ttttaaacta tcatagcata
     9541 agtttttgat attttcaatc tattaagaat agtgctatta ttaaattaac aaaagagaat
     9601 agctggtatg gtttagagta aatcaatcta tattacagtt atatcaacga atatcaaaaa
     9661 attatctatt aaaaaataaa aattattt
//
LOCUS       DAMJWU010000121         9357 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMJWU010000121 DAMJWU010000000
VERSION     DAMJWU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.126"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(123..776)
                     /locus_tag="PC460_003204"
     CDS             complement(123..776)
                     /locus_tag="PC460_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2602065.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPIAEMKKEKLL"
     gene            complement(1021..2886)
                     /locus_tag="PC460_003205"
     CDS             complement(1021..2886)
                     /locus_tag="PC460_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HDF2602066.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2891..5311)
                     /locus_tag="PC460_003206"
     CDS             complement(2891..5311)
                     /locus_tag="PC460_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF2602067.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     TEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5325..6767)
                     /gene="glgA"
                     /locus_tag="PC460_003207"
     CDS             complement(5325..6767)
                     /gene="glgA"
                     /locus_tag="PC460_003207"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF2602068.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6796..7926)
                     /gene="glgD"
                     /locus_tag="PC460_003208"
     CDS             complement(6796..7926)
                     /gene="glgD"
                     /locus_tag="PC460_003208"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF2602069.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7928..9088)
                     /locus_tag="PC460_003209"
     CDS             complement(7928..9088)
                     /locus_tag="PC460_003209"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF2602070.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2958 a         1711 c          911 g         3777 t
ORIGIN      
        1 atacattatt ctatcgtagt gccttagagt ttttatttgt atttaaagtt aaatttattc
       61 ttgtttttcc tttaatattt aatagtaaaa caacaaaatt ataaaaaaaa taaagagaaa
      121 ctttataaaa gcttctcttt tttcatctct gctataggta tcgttttcca atgttctctc
      181 aaaattcttt caacattttc taaatcattc tccctcatag attttactac ttcaatatgt
      241 tgattattgc tgtatgtcaa cattttatat aactcatctt cattatcact agtatatgac
      301 tttgacataa aattatatct taaagagttc aatagattta ctaacacctg attatctgat
      361 ttagttatgt atatatcgtg aaattcatta cttaatttta aatagctatt gtaattttta
      421 tactttatag agaccgccat catttctgtt aattcttcca tttttactag gtctgattca
      481 gttaaatttt gtaatgctga agaaccggct aacgcatcca atactccaat tacggaatag
      541 gtgtctaatt tctccttttc ctgaaattct tttactacaa atcccttatt aggtattttt
      601 tctagtaaat tttcacctgc taacctagtt aatgcttctc tcgctggtgt tctactaact
      661 ccgatagatt tacaaatctc agattcagta attctctgat tagcttcata ttctccattt
      721 tggattttct ttgatatata gtcatatact tgagttgtaa gtggatctac cttcataaga
      781 cgttcctcca tattaagatt ataaaagtta aatctcccta caactttggt agagagttca
      841 gttttatctt actaatttat ataatatcac aattatgata ttttttatac ttctttgtaa
      901 tattttgaaa tactaaaatt cttatgataa tttatacaaa aaaagtagaa aaagttttga
      961 taaaacaaaa ctctttccac attcgcctta ttttctattt atttttctaa aaaaagatat
     1021 ttaatttact ctaaaaattc taaatgattt agagtctaaa tctactttca actcattatt
     1081 ttcaatttgt atagtactat ttgcattttt aagactatac tttataggta tttcctcaat
     1141 aaaactactc tctatacata atgatatact ttccacattt tcacttctat ttactaaaat
     1201 taacattcta tcattatttt cattaactct aataaaagaa aatacagaat cattattagt
     1261 atatataaat tctgtatttc cagaacttaa aagtttattc ttatttctaa tttttataac
     1321 atctctataa aaattaagca tatcttcgtc ttcatttttc catggataag ttcttctatt
     1381 atctggatca acacctccac aaagacctgc ttcatctcca taatatatat atggtactcc
     1441 ttcaaatgtc atctgtatag ctacagcaag cttcactaaa tctacatcat catttaattc
     1501 cgttttagct cttgtaacat catgtgttcc aattaaattt aaattagacc taaatgattc
     1561 ttttggataa ttttctttaa tctgcatata tctattacaa agttcatatg aattaatttc
     1621 tccttttaag aaagaaaaca tgttatttct aaaaggataa cccataacag aatctaattc
     1681 ttcacctaat aaataacttc ttctctgacc atagctaatc ttatttgatg catcttccca
     1741 tacttcacct ataagaattg agtcatcatc tgcctttttt aattcctttt ttaactctct
     1801 tataaactta gttggtaatt cgtctgcaac atctagtctc cagcccttaa tacccatatt
     1861 tacccattta tttataacgc tatcattgtc atatattata taatccatat agctatgttc
     1921 taattcattt atatttggca atgtcttaac atcccaccaa cttttatatt tttgaggaaa
     1981 ttcttcaaac atataccatg aataatatgg agattcttta gattgatatg ctcctaaact
     2041 attatagttt ccatacatat taaaatattt actatctgct ccagtatgac taaaaactcc
     2101 atctaatact attgatatac ctttttcttt tgctttatca ataagttctt taaatgtatc
     2161 ttcatctcca aacattgggt caattttttt atagtcacct gtatcatact tatgattact
     2221 agaggcttca aatatagggc ttagatatag tatacttaca cctaattttt ttaaatatcc
     2281 aagtttattg attatccctc ttaaatttcc accatggaaa tcccatctta taacatcccc
     2341 ttgactatcc tttatgtaca taggtgtatc ttcccaattt ccatatatga aactattttt
     2401 tttaggatta tcaaccttac cactacgatt tccattattg aatctatcta caaatatatg
     2461 ataaagtatt ccttctttgt accatgttgg tactttaaaa tctttataaa cagtgatttg
     2521 atatttatta atatcactat aattatattc acaagaatat ccattatctc tgttttttcc
     2581 ataaaataaa gtctttattt gaccatccat ttcaacatca actttaaagt aataaaaata
     2641 aacacctgta ccatcaaact tttcaatctt accacaaaag cactttccaa gtttatcatt
     2701 atctaattct tcatttaaag ttattgttct catttcttct tctctattta tttctaggga
     2761 aatagacttg atattgtatc cctcatttac tttaacacaa atggtcaatt cctcatcaaa
     2821 ttttaaagct ccaaacggag ccttaaaatt tttatcccaa gagttatatt ctattacact
     2881 ttgcatttaa tcacaccctt ttaaaccaaa tgtcttttgc atactcagaa attgttctat
     2941 cagaagaaaa aaatccagag ttagctatat taactaaaga cattttattc catttttctt
     3001 tatctctata taacctgttt atatctgctt gagcttgacc ataattttca aaatctctca
     3061 atacaaaata ctcatcattg taagtagtga gtgaattata aatacttctt ccttcttcgc
     3121 caagattagg aataaatcca tttattaaat catctactac cctttttata tccatattag
     3181 aatgatatag gtcaagtgaa gaatatccac catatttatt ataatttagt acttgttctg
     3241 cactaagtcc aaataaaaac atattttctt taccaacctg ttcacatatc tctacatttg
     3301 caccgtcaag tgtagccaaa gttatagcac cattcatcat aaacttcata ttacttgttc
     3361 cagatgcttc cttagttgta gttgaaattt gctcactaac atttgctgct ggtattatta
     3421 tttctgctaa tgatactcca taattctcta aaaatactac ttttaacttg tcctttactc
     3481 taacatcatt atttatagta cttgcaacag aatttataaa tttaattata catttagcca
     3541 aataatatcc tggtgctgct tttgcgccaa atataaaagt tcttgggtca agattcaaat
     3601 taggattatc aagtaattca tgatacatat gaagtacatt aaatatattc agtaactgtc
     3661 ttttatacgc atgcaatctc ttaacttgaa catcaaatat cgaacttggg tctacattta
     3721 aatcgtattt atcttttata aactttgcta gatttgcttt attattgtat tttatatcat
     3781 caagtctttg taaaacagag gaatcatttt taaatttttc aatatttttt aattgtaatg
     3841 tatctttctt ccatgaatca ccaattaaat ctgtaattaa attgctaagt tgaggatttg
     3901 aacttataag ccatcgccta tgtgcaattc cattagtctt attattaaat ttatttggtt
     3961 catcttgata gaaatctttt aatacctctg tttcaagaat atgagtatga agcttagcaa
     4021 ctccatttac actatgactt gtgacaatac ataagtttgc catattgata ttgtcacagt
     4081 ctattatact cattcttttt attctatctt ggtcatatcc tttattattt aattcctcta
     4141 catatcttct atttatttct tctattatca tataaattct tggtaatagc tctttcatca
     4201 tacttacagg ccatttttcc attgcttctg acattatagt atgattagta taggacactg
     4261 tttttttagt tatttcccat gcttcatccc aagataaatt ttcttcatca agcaaaattc
     4321 tcataagttc tggtatgcat aaggtaggat gtgtatcatt tatatgaatt gctaccttat
     4381 cgaataaatt attaatattt aatttgttct ttttatattt tcttattata tcttgtatcc
     4441 ctgcacttac aaaaaagtat tcttgtttca acctcaacaa cttacctgca tagtttgaat
     4501 catcaggata taatacctgt gatatttcct cagtataata cttatgtttt aaagcctctt
     4561 catagctacc agaataattt agagcatttg aggtaagttc accaaaatct ctctttggta
     4621 tttcactctt aaacaatctt aaagtattta tacattggtt ttggtatccg attattggta
     4681 tatcataagg cattgccatt acaggaaggt agtttttatg aattactttt aaatgactac
     4741 cttccttgat taattctacc tcaccaccaa acttcaccat tgttgcttca tttggtctta
     4801 cagtttccca tgcatatcgt ccttctgtaa gccagttatc aggcacttct acctgataac
     4861 catttacaaa tttttgttca aacagaccat atttatacct tattccatat ccttgaccac
     4921 ttatgttcaa agatgccata gaatctaaaa agcaagctgc taatctacca agtccaccat
     4981 ttcctaaggc tgggtctact tcagtttcaa ttaaatcatc caagttgatt cctaattcag
     5041 ataaaccttc tctaacttct tcttcaatat ttaaatttaa taaattagat tttaactgtc
     5101 tccctattaa aaattctaaa ctaaaataat aaacttcttt tttcttatct aactttgtag
     5161 caacccattt ttttgctatc tcatctttca atagactaca taatacattt aacaattgtt
     5221 catcagtagc ttccttgata gattgtgcat ataagctata catttttact tcaaattttt
     5281 gcttgaattt ctttttgctt attgttacca aaaaaaccac tcctttaatc acatatgtct
     5341 ctatatgttt ctatatactc cttagctgat tttttccaac tattatctgt tttcattgca
     5401 ttttcaacta atttaatcca tttttctttg tccttaaata ctttgattgc atttttcaaa
     5461 cagaaaaaca tctcatgagc attgtaattt ttaaaactaa atccattgcc ttctccagta
     5521 aatttattat aaggtgttac tgtatctcta agccctccag tctctctaac tattggtaaa
     5581 gaaccatatc tcatagctaa catttgacct ataccacaag gctcaaataa tgatggcatt
     5641 aagaacatat cacttgcagc atatatctgt tgagccaatg atgcattaaa agtaattcta
     5701 gctgatactt tatctgaaaa attagaatcg taatagttga acattgattg atattgatgt
     5761 tctccagttc ctaaaacgac aatctgtaaa tcttctctca taagttctgg catcatgtat
     5821 gctattaaat caaatccttt ttgagaaact aatctcgaaa ctatacctat caacggaata
     5881 gatgaatctt ttttaagtcc caatatatct tgtaatctca atttattttc tacttttttg
     5941 tcaattgagt ctacatcata atgaacaaat atatctttat cttttgcagg gtcatttaaa
     6001 tcataatcta taccatttaa tatacctttt aattttcctg actcttttct aagtaatcca
     6061 tctaattgtt caccataaaa agatgtttgt atttcatttg cataagtagg actaacagtt
     6121 ataattttat ctgcaaaatt tataccagct ttcataaaat ttattgagcc ataatactct
     6181 atatctccat tatcaaagta tttttgtggt aagtccaata tatcatataa aacatgctta
     6241 tcaaatacac cttgatattg gagattatgt atagtataca tagtttttat atttttatac
     6301 ccctcaaggg ttgcatatct ttcttttaga attaatggta acatccctgt atgccaatca
     6361 tttatatgta ttacatctgg ataaaaatca attctactta tagcttctaa aactgcatct
     6421 gtaaagaaag tatatctttc tgcatcatct ccatatccat agatactctt tctttcatct
     6481 tctcttctaa aataaaattc attatctata aagtaaaatt ttacaccatc taattccatt
     6541 tctaaaagac cacagtattg atttctccat ccaacacgta cactaaacac agctatttct
     6601 tttagttgat cttttaaata acttggtata gttgaatatt tgggcattat tacccttaca
     6661 tctacacctt ctttttttaa ttctttaggc aatgcataag aaacatctcc taatcctcca
     6721 gtttttgcaa agggccaaca ctctgctgtt acataaaaaa ccttcattag tcattccccc
     6781 ttctaaaaaa tttatttata tatttgtatt cttttccact acaagaggat atttttcatc
     6841 tcctcttaat tctttcccat ttgatacact cgtattttta tctaatattg cattatatag
     6901 ttttgcattt gactttatta cacaattttg cattactatt gagttctcta taactgttcc
     6961 tttttcaaca tttacctttc taaatatgat actatctttt acagtacctt ctattaaaca
     7021 accagatgct ataaagctat tttctacttt agcactatct gtataaattg taggtgattc
     7081 attcttttct tttgtaaata tctttctttc agaatataat aattcatctg caacttctaa
     7141 atttaataaa tctttgctca tttcaaaata tgattcaaca gaatttacac attttagata
     7201 tcctgtatat tcataggctc ctattttcat attttctgct tcttcaataa taaattcttc
     7261 taaataacaa taattgcctt tatttacaat gttatatata cattcaacaa aatcttcacg
     7321 cttcataata tatgtctcca tacatatgtt tgctctaggg aacttaccta aattatttcc
     7381 tacatttatt accctattgt tactatctaa atttagagtt aagttaccag caaaatcttt
     7441 atttgcatta tttacatttt tatatattat agttatatca ttatgagatt ttttatggta
     7501 ttccaatgct tgagaataat ttaaattgca tagcatatag cttggagcta ttaaaatata
     7561 ttcttcttca ctcttttcta tataatctag attagctaag atagtatata tatcaccttc
     7621 tctatgtgag taagtatttc tatacttagt gttttctgga ctaaaaattg ataggccacc
     7681 tttttttcta cttaaatccc aatctttacc actacctata tggtctgtta atgatctgta
     7741 tttttctttt gcgaatattc caactttagt gattcctgaa tttatcatat tagataaagc
     7801 aaagtctatt attctatatc ttccagctat tggtgtagat gctattggtc ttccatagtt
     7861 aagtttgttt attgctgggt ctcctttttt gtttaaattt attatcccta aacattcatt
     7921 tctcatctta aagacctcct tctattaata cgcatctagg ttcgatattt tcatactcag
     7981 aaactacatt tatttcatta tttacattct ttatctgaac attatcattt atagttgcac
     8041 actcacctat catagctttt tctatagtta cattttcacc tattacaaca tttggcatta
     8101 ttactgaatc aattacttta cttcctttac caacaacaac tccatgtgat aatacagagt
     8161 tacctacttc acctaaaatt ctacaaccat cagctatcat tgatctatga acatttgcat
     8221 ttttacctat atattgtgga ggcattgcca tagtatttgt atatatcttc cacttagggt
     8281 ctgctaaatc aagtgtttct ggagatttta ttatatccat attagcatcc catagacttt
     8341 gtatagttcc tacatctctc cagtatcctt taaatggata agcatacatt ccaacattat
     8401 cttctaacat tttaggtatt aaattttttc caaaatcatc ataatttatt tctggatttt
     8461 tttctgcttc cttaaaataa tttctcaaca ttttccaatc aaatatgtat actcccatag
     8521 atgccaaatt gctcttaggt tcacttggtt tttcttcaaa ttcatagata cttgaatctt
     8581 catttgtatt cattatccca aatcttgaag cttcatccca aggaacttca atcactgcta
     8641 ttgtagcttt agagcctttt tccttatgaa attttaacat tttgctataa tccatcttgt
     8701 atatatggtc accagataat atcaatacat attctggatt ataggtatct acaaagtcca
     8761 tattttgata tatcgcatgt gctgtaccat tataccaatt tccttctttt tcattcataa
     8821 atggttgtaa aacatagaca ccaccattta ttctgtccaa atcccaatga cttcccatgc
     8881 ctatatgtga atttaatatc aacggtctat attgagttaa aacacctaca gtatcaatac
     8941 cagagtttga acaattgctt aacacaaaat ctataattct atacttacct ccaaatgata
     9001 cagctggttt ggcaattctt tttgtaaaaa cacctaatct tgaaccttgc cctcctgcca
     9061 aaatcatagc taacatctct tttttcattt aatgacactc ccctcatcta gtaattaata
     9121 tttataattg catttttact tttataaact ttatcagtca ataaatgtcg tgtataaatg
     9181 ttgcttaaat aataccttaa taaaaataaa ataatatcaa ctatacaatt gatataatgt
     9241 ataccccata atggacttac cgacgtatac attattttcc ataagcacta tagtatttat
     9301 taatattcaa ttattggtta aaaaataata caaattttat tttataaata taaaaac
//
LOCUS       DAMJWU010000122         9353 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMJWU010000122 DAMJWU010000000
VERSION     DAMJWU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.127"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            393..1598
                     /locus_tag="PC460_003210"
     CDS             393..1598
                     /locus_tag="PC460_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2602071.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1669..1890)
                     /locus_tag="PC460_003211"
     CDS             complement(1669..1890)
                     /locus_tag="PC460_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602072.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2031..2630)
                     /gene="recR"
                     /locus_tag="PC460_003212"
     CDS             complement(2031..2630)
                     /gene="recR"
                     /locus_tag="PC460_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF2602073.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2643..2993)
                     /locus_tag="PC460_003213"
     CDS             complement(2643..2993)
                     /locus_tag="PC460_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF2602074.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="PC460_003214"
     CDS             complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="PC460_003214"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF2602075.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4766..5030)
                     /gene="ffs"
                     /locus_tag="PC460_003215"
     ncRNA           complement(4766..5030)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC460_003215"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5068..5159)
                     /locus_tag="PC460_003216"
     tRNA            complement(5068..5159)
                     /locus_tag="PC460_003216"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5123..5125),aa:Ser,seq:gga)
     gene            complement(5160..5615)
                     /locus_tag="PC460_003217"
     CDS             complement(5160..5615)
                     /locus_tag="PC460_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF2602076.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5936..7207)
                     /gene="serS"
                     /locus_tag="PC460_003218"
     CDS             complement(5936..7207)
                     /gene="serS"
                     /locus_tag="PC460_003218"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2602077.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7333..7538)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7666..8547)
                     /locus_tag="PC460_003219"
     CDS             complement(7666..8547)
                     /locus_tag="PC460_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2602078.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8559..9068)
                     /locus_tag="PC460_003220"
     CDS             complement(8559..9068)
                     /locus_tag="PC460_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF2602079.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            complement(9194..9310)
                     /gene="rrf"
                     /locus_tag="PC460_003221"
     rRNA            complement(9194..9310)
                     /gene="rrf"
                     /locus_tag="PC460_003221"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2982 a         1642 c         1002 g         3727 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttaattttt ttatttatca tgtattcttt atctttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataatata
      361 aatactatgc tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg
      421 cttccactgg tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca
      481 aaacacttga tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata
      541 atacaatgga taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag
      601 cctatggaag tgtatataga ttatctgaaa caaatctttt gtcaaaaaat gaatttaaag
      661 ataatctact aataacattc atggctaaaa aatttaaaaa gcttattaga agcgaaaaac
      721 cagacttgat tataggaacg catccttttc caatgattgc tctaagtaca ttgaaaaaaa
      781 actttaattt acataataat gaatcaaatg cttacacaga gcatttttat aaacattata
      841 caaataccat taatgttcct ccgcttattt ctgtattaac cgattatact actcattcta
      901 catggataca aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat
      961 tggtgtttga tggagttgaa ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat
     1021 cttttctctc tcacagagat aaagatattg ttctttctga gttgaattta tctcctgata
     1081 aattaactgt tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg
     1141 atgaattact ggatactgat agagactttc aaatacttgt aattactggt aagaatgaaa
     1201 gcctaaaaga aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg
     1261 tccttggata cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca
     1321 aacctggtgg acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt
     1381 attatatacc aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac
     1441 ttagaactac aaaaaaatat aacttgtcaa tattactaaa agttttaata gatgacccta
     1501 gcagattaga aatgctaaaa agaaatatta aatcaataag aaagtcaaat tcagcccaaa
     1561 atatagctaa tttaatttta aatataattt ctggttgata aaataaataa aatccctata
     1621 gtatatatat tatctattag ggatttttta ttttctaatt atttaatatt aaattggctc
     1681 ataaaagtct tcttcataat catactgata atttttataa ttatttctaa attcagcatt
     1741 tttatttcgt acattttttt tagttttttt cttattagaa aaacttatat cctcaaaatc
     1801 atcatattta ggtttatctt ttgatttcat ttttttaact cttttttctt tcattgcatt
     1861 atgttcttct aagttcataa ttggtttcat gttgcacccc tatcaaaatt tttaaatgat
     1921 ttatacaaca aattatacaa tacagaagaa atttattcaa taaatctaaa tcaaaattaa
     1981 tttgaattta actcaaaaaa ctacaaaaat cgaggtatta acctcgattt ttatatttct
     2041 cttctgcctt ctagtgcttt tgatattgtg acctcatctg catactctaa atcgcctcca
     2101 acaggaagac catgagctat ccgagtgact ttgattccca ttggttttac taacctagca
     2161 atatacatgg ctgtagcttc accttcaata gtaggatttg ttgccattat tatttctttg
     2221 acttcctgat tgccaagtct ttggataagt tcctttattt ttaacatatc tggtccaatt
     2281 ccatccattg gagaaattac tccatttaat acatgatact gtcctttaaa ctctctcgtt
     2341 ctctccatag ctgctacatc tcttggatct tccacaacac atataacact tgaatctcta
     2401 cttttatttg aacacatgct acatgggtct gtatctgtta tattacaaca aattgagcaa
     2461 tatcttattt ctctttttgc atctataatc gctttagata aagcttccac atcattagta
     2521 ttcatattaa taatatgaaa agccaatctt tgagcagtct ttcttcctac tccaggaagc
     2581 tttgaaaatt cctctataag tctcgttatg ggacctgtat aaacttgcat aaataccacc
     2641 tactagaata acccaggtat attcatacct ccagttacct tactcatttg acttgcttgt
     2701 atatcatcaa tatttctcaa tgcttgattt actgcactta aaactaaatc ttgtagcatt
     2761 tctatatcat ctggatcaac tacttctggc ttgatagtta tatctataac ttctttttta
     2821 ccattaactt ttacagtaac tgctcctcca ccaactgaag cttctacttc tttactttct
     2881 aactcttgtt gagctttttg catattttct tgcatttttt gagcctgctt taacaagttg
     2941 ttcatatttc caccaccagg catcattccg cctccaaaac cttttttagc cataaacact
     3001 attcctccta aacttatttt cattttatta ttatatcata atagtatttt attatttact
     3061 ctcattttca tcaatactat cttttatttc taatatctct tcactcacta tgttctttaa
     3121 tatttcctca ccaatgtcct ttttctcttt aacttcaaga tttatatctt taacttctga
     3181 tgtcagtact atttttacat taaaacttct atttaaaaca tctcttattg ttttttctaa
     3241 ataatttatt gtatcttgag agctcagtct agtttttgca aaatcaaatt tatcatcaaa
     3301 tataacatac aacatattag aatatacatt aaaacttttt acttcactta agagtgcgta
     3361 tattggcatt tttttatctt ttttaatttg tttaagtata ttcttccaag atgactctat
     3421 aagtataaca tcctcatttt ttacatcttc ataaactaca ttttcttgtt ttacatctat
     3481 ttctctatcg acctcatact ctttttcttt attattccca attttttcag acttaaagtt
     3541 ccccagttca atcatttttt ctaagttctc aactcttttt ataagagctt ctttactttc
     3601 atcaaacatt ggttgagcaa ttttcattat ggttatttcc actaaaaccc ttggatttga
     3661 agatgacttt atatcatcct gagttattga taatatattt aaaatcctta ttaaatcatt
     3721 tatatctata tttttagact gaattttaag ttgttctata gtttcttctg gtaatgaaat
     3781 tatttcatcc aattctgatg aaaccttaca taccattaaa tttctaaaat gatcaattaa
     3841 gtcatttata agatttctta tatctttgcc ccataagaca aattcattta atatctcaag
     3901 agacttttta gtgtcctgct ctacaataca ttgagacaat tcaaataatt gttctatatt
     3961 tacgcttccc attaattcaa caacatcttt ataatctatc ttactttctc caaaagacat
     4021 acattggtct aatatactaa gtgcatctct aagagctcct tgagaatttc tagctattaa
     4081 attaagagct ttatcatcta tatcaattcc ttcatcttca caaatctttt tcattctaag
     4141 ggtcatatct ttaactgtaa ctcttttaaa atcgtatctt tggcatcttg ataaaatggt
     4201 tgcaggtatt ttgtgaggct ctgtagttgc taatataaaa attacatatg atggaggttc
     4261 ctctaaggtc tttaataatg cattaaaagc tccttgagac aacatatgaa cttcatctat
     4321 tatatatact ttatatttag cattggctgg agaatatttc acactttctc taagctctct
     4381 tatatcatct acactattat ttgaagctgc atctatttct acaacatcca ttgtattatc
     4441 ttttaataca gattcgcata tctcacactc attacacggt tcttcattta ttttattcaa
     4501 acagtttact gctcttgaaa aaatctttgc agtagatgtt ttaccagtac ctcttgttcc
     4561 acaaaacaaa taagcatgtc ctatattatc actatatatt tgattcttta aagtcttaat
     4621 aatatgatct tgaccaataa catcttcaaa tttttgtggt ctatatgctc tatataatgc
     4681 tttatgcatc tttttacctc tctttagcaa aattaataaa aaaatccctc attatctaag
     4741 ggattagtct tttatatatt aaatgctgta caccatctat cgacttaatt ccataggcgt
     4801 taccaaaaca gttagctcaa cttaggcacc cccacggcac acaaaagtga tcacttaccg
     4861 ctgcttcctt ccggatctga cggggttcat gagctttcat tgcgcaggac ctaagcatca
     4921 acaccactta tttagggcgg ccctacagaa aataagcctt tagatggaat tcaatcctgc
     4981 tatagcggat tgcaggttac agggcaccgc tacctcccca tctagtacag caaaattaca
     5041 accatattta attactttca ttgtttctgg cggagagagt gggatttgaa cccacgatac
     5101 agttttaccc atatactcgc tttccaggcg agcgccttcg accactcgac catctctcct
     5161 taatctcttt ttctcaatgc cttaaaaaat tcttgcaata tgatcgagca ttcttcttgc
     5221 aaaacatcaa aagctacttt tacattatta ttattaaaat aatctaattt aaactcatgc
     5281 tgtttttcta tatatgaatt ttttacatgt cttgtcccta ttataagctt ttttattcta
     5341 gaattaacta ttgcgccact acacattatg caaggttcca ttgtaacata taaatcacaa
     5401 tcagtcaacc tccaaccacc taatttttct gatgcttgtt ttatcgctaa aatttctgca
     5461 tgtgccgtag agtcttttaa cgtttctgta aggttatgag ctctagctat tatttgatta
     5521 tctttaacta taatagcacc tattggtgtt tcttttttat tatatgcttt atatgcttct
     5581 tttaatgctt ctttcatata aaatgaactt tccataattc ctcccaaaac ttataacaaa
     5641 cttatcttat cataattcat tttataattc aacttaattt tattttgttt atttaattat
     5701 tactatatat agctatattt tttttaaatt agttctaaaa taatttttag atattcatac
     5761 aagagtgttt tataaaaata tttagtgttt tatgatgaaa attctgctgt gtaaattaac
     5821 ataaaaaaac tatgaaatta atatttataa taatttcata gtttttttat ttattgttta
     5881 ctcataatat attctcttca tcaaagcata ctctacatat acaattaata taaatttatt
     5941 ttataacatc aacacccata taaggtctta attcttctgg aactactaca gaaccatctg
     6001 cctgctgata attttctaat atagcagcca aacatcttcc tatcgctaat ccagaaccat
     6061 ttaatgtatg aacatattct gcttttgatt ttttatccct tttaaatctt attcctgctc
     6121 ttcttgcttg gaaatcttca aaatttgaac aagaagatat ttctacatat ctattgtagc
     6181 ttggcatcca aacttctaaa tcatatttaa atgcagctgt aaatcccaaa tctcctgtac
     6241 atatttttac aactctatat ggaagaccta acatttgtaa catagtttca gcatcatgag
     6301 ttaatgattc taattcatta taagaatctt ctggtttaac aaatttaact aattcaactt
     6361 tattaaattg atgttgtcta actaaacctc ttgtatctct tcctgctgaa cctgcttcag
     6421 atctaaaaca aggagtatat gcacagtatt taattggaag ctctgcaaca tctaatattt
     6481 cattagcatg tatattagta acaggaactt cagctgttgg tattaagaaa tactctaatc
     6541 cttctatttt aaacatatct tcttcaaatt taggaagttg accagtccct ataaaactat
     6601 ttctattggc cataaatgga ggtaagactt ccgtatagcc atgcttagct gtatgagtat
     6661 ttaaaaagaa attcattaaa gatctttcta atctagctcc taatccccta tataaagtaa
     6721 accttgaccc agttattttc cctgctcttt caaaatctaa aatacctaga cctgtaccta
     6781 tatcccaatg cgctttatgc tcaaaatcaa atttagtagg ttctccccaa gttcttattt
     6841 gaacattgtc ttcatctgtt tcaccttgtg gtacctctgg atgaggaaca tttggtattc
     6901 tcattaatgt atattccatt tttgcttcta cttcttttac tttctcatca atacctttta
     6961 tcttatctga taactctttc aatcttgcct tttcttctgt tacatctttt ccttctttta
     7021 ttaattgagg tattttttta gaatttacat ttaactcatt tttcataact tcaacttctt
     7081 gaagtatttc tcttctttta ttatctaatt ccactacagc atcaaggtca aattctcttt
     7141 cacctcttct ttccatagct tttttgatat catctagatt ttctcttatt cttttaatat
     7201 ctaacatatt aattcctcct aataattttg ataatataag ttataattcc aacttaattt
     7261 acaaacatta tacttgtaaa ctcttagttt aaacaaattt taaaaataaa aaaagacctc
     7321 cgtttaccct ataaaaggga cgaaaatctt ttatccgcgt tgccacccta attgactata
     7381 ctaaaatgta taatcctctc agacctataa cgtaggtaaa cgtctagctt taactagaag
     7441 ctctagagta gattcaaaaa ccattgtaat gagttttcac catccactca ttctctgaat
     7501 acaatctgtt ttttactatt ttccttcaca gctttttaac taattgtttt atttgataat
     7561 cattatatta tatatattaa catctttcaa tactattatt caaatttttt tagaattttc
     7621 tttaattttt tatattaaat tcattttttc tatttaatta agaccttatc ttttatccat
     7681 tgtatttaga ttacctgtat tattaaataa taatgaagaa ggaacaaatg gaacttttat
     7741 atcattctca tcaaatttat tttttacctt ttccattaca tcaaagtata catcccagta
     7801 atcatcagtt tttacccaag gtctagcaac tatttgcatg gtgttatttc ctatagagtt
     7861 tagacctatt ataggctttg gatcatttaa tattcttttt tcgtttgtaa aaatttcatt
     7921 taatatagac ttaactacat ctatatcact atcatatgaa atattatatg aaaaatctat
     7981 tcttcttttg ttttgatgtg tgtaattaat tatattatta cttgtcagtt gaaaattagg
     8041 tataattata gttttattat ctggcgtctt aagaacagtg ctaaatattt gtatgtcaga
     8101 aactgtacct tctacacctg aaccttgaat ataatcacct atattaaatg gcttaaaaaa
     8161 taaaataatc ataccagagc ctaaatttga taatatttct ttaaaagcta aacctatact
     8221 aaatccagca gctcctaaca cagctacaac tgatgtagtt tgtatcccta taatacctaa
     8281 gcttatcaat gatataatag ataacataga tatatatatt ccatatacaa taaaattatt
     8341 tacaccttta ctcattccat atttgattag aaactttgaa accatccttc ttactatttt
     8401 agatatataa atacctatca caaatacaat tattgcatat ataatatttg gtatattatc
     8461 actaaaatta tcgagcatat tttctaaagt tctcttagtt atattgctat ttgttgaggc
     8521 agtcttagcc aatgataaaa aattcattca actcctcctt atgataatat tccgttgtcc
     8581 tttaactctt ttactatagc ttctatagtt tcttctttta ttgcctcaat tgtatgaata
     8641 tggctacatt ctgtcagaac cgataattgt ttaaattcat ttgtctcaac tttcttcata
     8701 aagttgtcta tatccatttt agaataaata ttaagttctg cagttatctg accatatgtt
     8761 ggatgattta caataacatc ttttataata ccacctaaat ctacaattat ctttaattca
     8821 tcataaattt ctttagaatc cttatgattt ttacacttta tttgttttgt aaaatacggt
     8881 tgattatata ttatatatcc ttgaggtgta gctattattt caaatcctct agctcttata
     8941 agggctatat cttgaactat tacttgccta cttacattta atagttcagc tagtgttcca
     9001 ccttttacag gatgcttaga ttcttttaaa atatctatta atttacttct tctttcatta
     9061 gaattcatat tacaccttct ttccaaataa aaactttacc tatactatat aattttatat
     9121 tttatcttat tataaatcaa tattgacttt atattctatt taatcttaac ttttataata
     9181 aataaaaaag actacgtggc tacgtcctac tctcccaggc agtttcccac caagtaccat
     9241 cagcgctaga gagcttaact tctgtgttcg gaatgggaac aggtgtatcc tctctgctat
     9301 tgtaaccaca taatctttat gcttaatatt tctattaagt ttttaataag ttt
//
LOCUS       DAMJWU010000123         8981 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMJWU010000123 DAMJWU010000000
VERSION     DAMJWU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.128"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(39..647)
                     /locus_tag="PC460_003222"
     CDS             complement(39..647)
                     /locus_tag="PC460_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF2602080.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(756..3494)
                     /locus_tag="PC460_003223"
     CDS             complement(756..3494)
                     /locus_tag="PC460_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2602081.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINVCISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3642..4433)
                     /locus_tag="PC460_003224"
     CDS             complement(3642..4433)
                     /locus_tag="PC460_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2602082.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4469..5125)
                     /locus_tag="PC460_003225"
     CDS             complement(4469..5125)
                     /locus_tag="PC460_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602083.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5118..6083)
                     /locus_tag="PC460_003226"
     CDS             complement(5118..6083)
                     /locus_tag="PC460_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF2602084.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKNLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6189..6295)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6585..7529)
                     /locus_tag="PC460_003227"
     CDS             complement(6585..7529)
                     /locus_tag="PC460_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2602085.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSASIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLVKSKVLELYNHHCI"
     gene            7640..8107
                     /locus_tag="PC460_003228"
     CDS             7640..8107
                     /locus_tag="PC460_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602086.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8277..8876)
                     /locus_tag="PC460_003229"
     CDS             complement(8277..8876)
                     /locus_tag="PC460_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602087.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2911 a         1490 c          868 g         3712 t
ORIGIN      
        1 taccttccta tagatacata tcttacctat ataaaaactt attctatata aaaacgccat
       61 aaaaaatctt ttgcttcttc tgcataatct atatttatac gtttactagt ttttatatct
      121 aactcatctt tactaaatac tttttttcct ttatcatcat aataaaaatc acttatatat
      181 aattcttcac ccattatact cttactattt agacttctat ctatttttaa agctttgcaa
      241 agttttcctg gtccgttagt aatatttttt acttgatatt tactcaactc agtgtaagct
      301 ttgttgtatc tattcataga tatctcatct aaagaagtta taggctctac ccctcttatt
      361 aatacacact ctggcacatt ttctatattt gcagataaat tcaagcaatt atacatacca
      421 taaattaaat aaacatatat atgtccacca tctaaatata atggcttagt tctatctgtc
      481 tttttattgc catatacatg tgctccttta tcatttacac ccatgtatgc ttctgtctct
      541 atgattttgg taacaattac tttattttca tatttcctta ttagatattt acctaatatt
      601 gatttggcta aatcaattcc attttttcta aaaaaatcct tttccaaaat ttcacctcat
      661 aaaatttatt taaaacgcat caaatacaac taaagtaata aataaaatta actcttatta
      721 atgtgatata tataaacaat aaatttagac atttattatt tatatatatc aaaaaattct
      781 atctcaaact tttcaatatt atcattgtaa tttgaattat catatacttc ttcaacaacc
      841 tctttaattg ggaatataac ttttacatta gttccttctc ctaccttact atctatgtaa
      901 atcttcccat tttgaatttc aatcaatgat tttgtaaggc ataaacctat accacttcca
      961 aattcatttg atataccact gttttcaatt tgtttaaatc gattaaatat atcatttaat
     1021 ttttctttgt ctatgcccac tccagtatct tttataatta tctctactgt ttcgtctttt
     1081 ttatatatac ctacaaggat ttctcctcct tctaaagtaa attttatagc atttgataat
     1141 atatttagta ttattctttc tatcgattct gggtcaagcc ctaccataac agtttcttca
     1201 tctgtatcaa aaattaaatc tattccttta gacttagcat aactactaat agatgtaact
     1261 attctttctg ttatgtcaat aatatcaaaa ttaactgctt taatcttata tatgtctgac
     1321 ttaacttgag aaatatttat tatattatct attaatctca ataatctaaa catattttgt
     1381 ttagttattt ttatatgttc ttttagtata tcctgaaaat caattatttt tcctttttta
     1441 taaaggtttt caataagttg aactgaacta tatatattat ttaaaggtgt cttaagttca
     1501 tgagatatat tagcataaaa ctccatcttc atttggtcat actgtctttg ttttattaat
     1561 gcttttttaa gtctttcaga ctctctttgc acacttaaat ctttaataat tctcactcta
     1621 cttcttttct gtccaaagta taatgttctt gatatcattt ttacttcttt aatgtgatta
     1681 ttttcattta ctatatggat actcttctct ctttctccta tatttaaatt gtttatgtat
     1741 ttttttacta atatatttct atatttttgt gttgtaactc ctaatacatc actagaaatt
     1801 ccgaacaact ctctaaaggc tctatttgta tatagtactc ttgatgtact taaatcctca
     1861 agaaaaatag cctctggtat aacatccagt aatttttcat atattatttt ttgttcattt
     1921 actgttctaa gaatatttat ctctttagtt atatctctaa atgtaagcaa tttaaagagt
     1981 ttattatctg accaatagtc tccaactcta acttcttcaa catgctcttc accttctgag
     2041 tcagacatca caaaattaac tatacagttt gctgtgtcat tgtaacttac tatttcagct
     2101 ttcttttctt gtgttagatt taatctttta aattccctat tgtcaaagaa atctatgtta
     2161 aaagtttcaa aatattgttc taatgtcata tcgactaaat cacttgaaac acatttaaaa
     2221 ctcttttcat attttttatt tatacttatt accttattct tctttatgcc accttcagca
     2281 cttcttacaa gtataggaga tggaatattt agtataatgt tttcatagtc tttatttata
     2341 tttcttattt cttcatctat tttgatttca tttgtaatat catttaaact tattaagtaa
     2401 ttgctttcat ctttattatt acttaaactt aacaaataaa ttttatcatt taactctacc
     2461 agtttatcaa ttatacttat atcgttatca aatttcttca atttttcagc aactttttca
     2521 tcttccttta taaaagttaa tattttataa taattattca tatcctttga aaattcatct
     2581 ttaaatttat tatttgtgta acttatatta aaatcatagt ctgttactat ccatacattt
     2641 ggtattgtat ccagagcttg tctaagtaat tcttttctct ggtctatgta gttttttaat
     2701 gttctttgta taatcatatc cttttctatt gctaaattta tctttcctaa ttgctctgac
     2761 tttttactta attttttatt gattgaatca agttcataat atggatacac tactctatta
     2821 ttaagactgt atacatatac ataataagta tggttacata atagtaaata tattactcct
     2881 attaatctat ctgaatctac tatattaatc ttagaaattg ctacaaaaca tataatagtt
     2941 gtaaaaaaaa caaaatatgt taaatctttt attaacttat agtgctcaat gttactatca
     3001 agtttattca acaaatatag taaattaaac atagatatac aaacattaat taatataata
     3061 atcatattcc agttaatatc actaaaaata tttcttataa agtaatctaa tatcacaaaa
     3121 gcactataaa aaattagtat tgggcttatt cttagttttt ttactttaga aatggttaag
     3181 tatataaaag tagacatatt aattatgctt aagttaagca atacaatttg gaatttttga
     3241 atttttaaat ttactactcc taaaatactt acaaaaacta atacaagaca ccctactttc
     3301 aatatattta taaattcatc tgttttaaag ctacttatat aaactataga atatccaaat
     3361 atccaaagta aaattattct atacatattt atagaaactg tatacaaatt tatatttaaa
     3421 agtgcaataa ccaaaaataa cccattagct agtatataga ccttattctt acttaaagtt
     3481 cctttagatt ccatattccc caccacttct tcatttttat atagttttac ctataaatat
     3541 gttaccacaa ttcttccaaa aaatttgtca ctttctagtt ataaaaaaaa atgagtaaat
     3601 atttttaatt ttactcattt tgtagatttt gtatattcag tttaaaaagc tggaactata
     3661 cttcctttat attcatcttg tataaatttt ttaacttctt tactattcat agcctctgat
     3721 aaagccttaa tcttatcact atctttatta ttttctctac aagctattat atttacatat
     3781 ggagaatcac ttgattctat tactattgca tccttagtag gatttaagtt agctgttaaa
     3841 gcataattag aatttattac tgctccgtca acatctttta atactgttgg taattgctct
     3901 gcattcattt cttttattac tatattttta ggatttttag ttatatcttt tttagttata
     3961 agctctccat cttttacttc tatcaattta ttttttgcta ataatttaag tgctctagct
     4021 ccattagttg catcatttgg cacagctata acagctccat cttttaattc atctaatgac
     4081 ttaacttttt ctgaatagaa tcccattggc tctatatgaa ctttagatgt ataagtaagt
     4141 ttatatcctt tctccttcac agtttcctct aaaaatggaa tatgttgaaa gaagttagca
     4201 tccaaagacc cttcatctaa agctgtattt ggaagtacat aatcatcaaa tatttttact
     4261 tctaaatcat atcctttttc ttttaacaaa ggttttgcaa cttccaatat ctttgcatgt
     4321 ggatttgatg aagctcctac tagtattttt ttatcctctt tgtttgaaca acctactgct
     4381 gatattgcta ttgctgacac caatgctact gataataatt tctttaattt catttttact
     4441 ccccctcttt aaaatgttta taattaattt attttagttt cttataagcc agatttccta
     4501 ctccctggat tatctgaact aatattataa gtgctattac agtatatacc attactgctg
     4561 tatcaaatct ttgatatcca taaataagtg ctatattacc taatcctcca gctcctactg
     4621 cacctgccat agctgtataa ccaagtatag atattattga taatgtaatt ccagatacta
     4681 ttgaaggcat agcttccttt atcataactt taaatactat ttgtcttttt gtagctccaa
     4741 aagattttgc tgcttctatc aatcctttat caacttcatt taatgagctt tctataattc
     4801 tagcgataaa cggtgctgcc ccaatagtta ttggcactat tgcagcagtt tcccctatac
     4861 ttgttccaac gattaatcta gttattggaa tcattgcaac tataagtatc ataaatggga
     4921 aacttctaaa tatatttact ataaatccta atgcactatt tattgtgcta tttggtttta
     4981 atccatctgg ttttgtaact actaatatga ttgctaatat aaatcctaat attgttgcca
     5041 ctatagttgg tactataacc atatacaaag tttgtaacaa tgcatttgga aatagtgttg
     5101 ttaaaaagtc aattaaacta ttcatttatc atttcctccc atcttatgcc cttactagtt
     5161 agatattgtt ttacttgctc tccactttta tctgaaatgt ttataataag tgaacctaaa
     5221 atatcatctt taaattgttc taatttccca aatattatag acacatctat atttagttct
     5281 cttgccatag tcgtaataat agcttcatta gaaatatctt tagggaatag tatctttata
     5341 tttgttcctt ttggcaatat tatactttct tcacctatta attttctaag tcctttagta
     5401 tttcttaaga atatttcttc tgtatctcct acctctaata cttctccatt ttccattatt
     5461 gctactcttc cacaaatctg ctttataact tccatttgat gagttacaac tattattgtt
     5521 attcccaaat ttttatttat atcttcaagc aatgaaagaa ttgactttgt agtatttggg
     5581 tctaatgctg aagttgcttc atcacaaaga agtacttgtg gatttagtgc taaagctcta
     5641 gcaattgcaa ccctttgttt ttgtccgcca cttagatttc tgggtttatc atttttcttt
     5701 tcagatattc caactacttc taataactct aaaactcttt ttttaatttc tgcctttgag
     5761 tatccaaaac attctaatgg caatgctaca ttatcaaata cagtttttct ttcaagcaat
     5821 gaaaagtgtt gaaaaatcat tcctaactct tttcttaaat ctctcatttg cttttcattc
     5881 aatgacttta cttctttatc agaaacaagt acactgcctt cttggtattc ttctaatccg
     5941 tttatacatc tcagtagtgt tgatttccct gcaccactat gacctattat tccaaaaatt
     6001 tctccagatt ctatttcaat acttacatct ttaagcactt gaatctttcc ataatactta
     6061 ttaacatttt ttatgctaat cataaaattt cctcccatat attctgctca gtttttatgt
     6121 ttatcctctc ttatgtatta caacaaaata aaagcccgag tataaactca ggcttagaaa
     6181 gctatttata ctcatctatc aactatatta gttgcaggat ttagcaccaa tgtatataaa
     6241 atatactggt tgccgggttt caaagggcca gtccctccac cactcttgat aagacgttat
     6301 ttaatttaat tctattatca tttgttgcta tgttatatat gatatattca ttaatagtat
     6361 ttgtcaataa atttatttaa attttttatt ttttattatc gttaattaac tatttttaat
     6421 tagttttcta gctcctaaca ctttatattt taatgtctat ataactatag aacatcatta
     6481 taagaaataa attaaataac atcaatctat ctaaatcatc tgtaaagtaa attaagaaaa
     6541 ttaacatttt atccaaactc tatcctacta ttaaataaaa taatttaaat acaatgatga
     6601 ttatatagct ctagtacttt agactttacc aaatgtttaa gttctatagg ttctattatt
     6661 gtagcatttt tcccaaaagt taaaaaatat tcagccaccc agttaagtgt agattttggt
     6721 atatacatat ctatgtatcc acctttagac aagattttca accctcttgc caacaaaaat
     6781 tctgtttcac aacgttttac tccctcatct gtcaaattaa ttttaatatg ataatctgta
     6841 cctacttcca tattcttaag ataatcatgt atagaagata aaaccttata tttcttactt
     6901 gataagtttt ctatactcaa aatcttcaca attctgtcta cacgaaattc cttaatagat
     6961 gctgatttta tacagtaagc tggacaatac caaagtccat tcatggcata caaaccaata
     7021 ggaacaatgg tacgtacact atttttttgc tttgatgaat attcaatttt aattgtagat
     7081 tcattcatta ctatattaaa caattctttc aataatggtg ttgaacagtg cctatctggt
     7141 gtccaaaaaa caaatttacg ccttatattc tcaatattat attgaatatc atttggtata
     7201 caatttaaaa atttttttaa aacagaaata gtttcttgct caaatggcaa atcattataa
     7261 tattctagtg actgatatgc aaagaatata gattttacct cactttctga aaatgcaatc
     7321 ggtggaagta tacgctcttt aagtacatga tatccaccag ccgctccaac ttttgaatat
     7381 aatggaaatc caatactctc cagcacctgt aagtctctta atatagtacg ttttgataca
     7441 gaaaattctt ctgctaattc tcctgctgta aaacttcttt ttgcatttat tgttatcatc
     7501 agttcaatta aacgctcagc tcttgacata tttatactcc taatttaaat atagtgacaa
     7561 taattgtcac tattatatga taagatattc ttacatttaa agcaataata atagtaaaga
     7621 aagaagggac tgatacttta tggaaaataa tactattaag gctgatgata ttatcaatta
     7681 ttgtctatca aacttagatg atgttttatt aatgaatagt tggggggaac gagcaattta
     7741 ctacaatcct aatggtattt taaagcgagg agtatatgtt cttaccatta aagaaaaaga
     7801 cagtaatcat gataaaggtt cgttggttag tcgcccaaat gtataccgtg tgaatatagg
     7861 attaaaaaaa gaaactttta ttgaaatgtt tggatatatt ccaaaacgtc caggtgtagg
     7921 tcaaatagtt gatatggatt ttgattttac aaaattggac acaatcatgc cacatcctat
     7981 ctactcatgg atgggatgga tatgcgtcct aagccctact gaaaagacat ttgagaactt
     8041 taaaacttta ataggagaat cttacaattt tgcaaaacaa aaatttaaaa agagaaaaaa
     8101 caagtaaaaa cgtatgaaaa taatccctaa ataaattata ataaaaatat taaggttaca
     8161 agataaaaat taatatctta ataacatgca aaaaagcgaa gccaaaaaat gtattattta
     8221 taaggcttcg ctatatctca aataatttat ttcaaatttc aaaactattt attctattat
     8281 ttcttattat ttcctgttat tcctaatcta tcattcatat cttttatcat ttttctaaaa
     8341 ccactaccta tatcatttcc aacatcttca gttccatgaa ctaattttat aaagtcattt
     8401 tgaccttttt tcatatcttc atcagatacc ttatctaatt tttctattct cttatctatg
     8461 tcttttacta atctttcccc ttcagcgatt aggtcatcat tcattttctt taactctgta
     8521 tcttctgttt taccatcttt tttaagagat gttacaaatg attgtatttt atctctacta
     8581 tctttatatg cagcctttac atcacttaga tatttttcat ttcctggata ttctttatct
     8641 ttaaactgat tatttatact ttctggagta tcataaatag aatatctatt tagacctgct
     8701 atataatcat tatagcttgt tgaataataa ttcatatatc cttcattatt atttacagat
     8761 tcattaggat tttttcctgt attttcactt ggtttttcat tatttttagt acatgcagtt
     8821 atacctatag ctaatactag tgctaatcct acaaataaag ttctttttaa tttcattttt
     8881 attaccctcc tagatgtttt atactataaa aaattttaat aattttcttt cttaatttag
     8941 tatttctata tttttatatt ttatttacta aaattaaaca a
//
LOCUS       DAMJWU010000124         8664 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMJWU010000124 DAMJWU010000000
VERSION     DAMJWU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.129"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            143..493
                     /locus_tag="PC460_003230"
     CDS             143..493
                     /locus_tag="PC460_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2602088.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            512..2155
                     /locus_tag="PC460_003231"
     CDS             512..2155
                     /locus_tag="PC460_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF2602089.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2236..4293
                     /gene="recG"
                     /locus_tag="PC460_003232"
     CDS             2236..4293
                     /gene="recG"
                     /locus_tag="PC460_003232"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF2602090.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4352..4906
                     /gene="rsmD"
                     /locus_tag="PC460_003233"
     CDS             4352..4906
                     /gene="rsmD"
                     /locus_tag="PC460_003233"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF2602091.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4908..5405
                     /gene="coaD"
                     /locus_tag="PC460_003234"
     CDS             4908..5405
                     /gene="coaD"
                     /locus_tag="PC460_003234"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF2602092.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5409..5933
                     /locus_tag="PC460_003235"
     CDS             5409..5933
                     /locus_tag="PC460_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602093.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6277..8196
                     /locus_tag="PC460_003236"
     CDS             6277..8196
                     /locus_tag="PC460_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2602094.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSIIELINKQN"
     gene            8209..8496
                     /locus_tag="PC460_003237"
     CDS             8209..8496
                     /locus_tag="PC460_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2602095.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
BASE COUNT         3595 a          889 c         1604 g         2576 t
ORIGIN      
        1 tttatccaaa aacttttcta ggtttaaatt ataccatatt tatagtatga tatgattata
       61 attttatata ataaataagt aaaaatagta aaaaataaat taagaaatgt ttttaataaa
      121 aataagctta agggggtcaa ccatgagtgc aaaggtaacg aatcaatatg gaaatataga
      181 aatagataat caagtaatag ctcaagtcgt atatagagct gcaatggaaa gttatggctt
      241 agtaggttta gcatataagt ctaaaggaat agtagagcta ttaaaaggcg agaacgcaac
      301 taaaggtgta agagttgagg aagtagaaga agatgccata gcaatcgaac tttatgttat
      361 aatacaatat ggtacaaata tatctactgt tgccaataat ataatagata gagtaaaata
      421 tgtagttgaa aaaatgactg gcgtaagagt tactagaatt gatattaatg tacaaggaat
      481 aagagttaag taatttcata ggaggaaaca aatgattcaa tatatagatg gtaaaaggtt
      541 aagggagatg tttatttcag gtgcaaacaa tctccaaaac aataaagagt tagttgacaa
      601 gttaaatgta ttccctgtac cagatggaga tacaggaact aatatgtcat taacaatatc
      661 ttatgcctta aaagaattag caaaagttga aaatgataat atatcagata tcggaaaagc
      721 attatctaaa ggttctttaa tgggagctag aggaaactca ggagttatac tttcacaaat
      781 aatcagaggt atagctaaat ctatagaagg aaaaagtaaa ttatcaacag aagatttagc
      841 aaaagctttt aaaaatggtt cagacacagc atataaagct gtaataaaac caattgaagg
      901 aactatactt actgtagtta gagaaagtgg agagtttgca ataaaaactg cgaaaaaaga
      961 gaaagatgta gtaaagtttt taagtatgct tgtgaaggaa tcaaattctt cattagaaag
     1021 aactccagac cttctaaaga atctaaaaga agctggtgtg gttgactctg gtggaaaggg
     1081 tcttgtactt atatatgaag gaatgttagc ttctataaaa ggcgataaca tagaaataaa
     1141 aaatgttgac ctagatacta atatttcaac aagtatggat tttgcaaaga gtactactag
     1201 cacagatgat ataaaatatt gctattgtac agaatttatt ttagaaagtt ctaaggtaga
     1261 agatactaaa ataagagaca taatgatggc ttatggtgat agcctagcag tagttggaga
     1321 tgatggagtc ataaaggtac atgtacacac taatgaccca ggaaatgtac ttcaagaagc
     1381 tctaaagtac ggtcaattat taactataaa aatagaaaat atgaagttac aacatgaaaa
     1441 tacattactg gatgtagaag aaaagaaaga aaatgatagt gaacctttag aagaagaaaa
     1501 agagtttggg tttatagcta cttctatggg tgaaggtttg gcaaatatat ttaaagattt
     1561 cggagttgac catataatag aaggtggtca aactatgaat ccaagtactg aagattttat
     1621 gaatgctata aagcatataa atgctaaaaa tatatttatt ttcccaaata acagcaatat
     1681 aataatggct gctaatcagg caaaagaatt gagtgataaa aatataatag ttatacctac
     1741 aaaaaatacg cctcaaggat ttgctgcatt agtaactttt aatggagaat taagcgaaga
     1801 tgaaaataaa gaagctatga tgaatgcttt aaactcagtt aaatcaggtc aagtaacttt
     1861 tgctgttagg gatacagtga tgaacgaaat tgatgttaaa gaaggtaata taattggaat
     1921 tgcagaaggg aatttattat cagcaggaga ttatgtggat gaagtaacat caaaccttat
     1981 tgaaaagttg gttgatgaag atactgctat aataacatta ttctttggtg aagatgttac
     2041 agaatctcaa gcaaatgaac tacgtacttc tttagaagaa aagtttgaag atgtagatgt
     2101 agagttatat tatggaggac aacctcttta ttattactta atttcagttg aataatgatt
     2161 ataagaacct gtatgaccaa gtagctttta taaaattggt tgtacaggat ttttgtgtag
     2221 taggtgatat atttgttgga tttatataaa gatgttcaat atgttaaggg aataggacct
     2281 aaaaaagcag ataaattaaa taaactgggt atatttacac taaaagacct attgtattat
     2341 tttccaagac aatttgaaga tagaaataat ctaaagaaaa tagcacaatt agaagatggt
     2401 gagaaagtta cgataaaagc agtaatatca agtataaata catttagccc aaaagaaggt
     2461 atgactttaa caaagattga tgtaaaagat gagacaggct cggctaaatt agtgtttttt
     2521 aataagagtt atataaaaaa tacttttcgt ccaggagatt caatattagt ttttgggaag
     2581 gttaagaaaa aatttaataa cttagaactt acatcatgtg aattagaata tcttactaat
     2641 tcacctaaga atacatgtag atttatgcct gtgtaccagc ttacatacgg tgttacaaat
     2701 aaagaaatta tgagtataat tagaacagta cttgaagata aagaattaat tatacaagaa
     2761 tatatgccac aaagaattat agaaaaatat aggctatgta gtatagattt tgcagttaga
     2821 aacatacatt caccaagtag taaggaatca ttaaaaatag ctttatatag aattgtattt
     2881 gaagaattac ttatactaca actagggttg tttgtattta aaagtggcag aaataaagaa
     2941 gatggaataa agtttgaaac aagtaaagac ttaaaaaaaa taataagtgc tttaccattt
     3001 aaactgacta aagctcaaaa cagggcatta gatgaaatta tacaagatat gaatttagag
     3061 aaaataatga atagattggt acaaggtgat gtagggagtg gtaaaacagt agtagctctc
     3121 ttagcacttg caaattgtgt tttaaatggt tatcagggcg ctttgatggc acctacagag
     3181 atattagcag ggcaacatta tatttcactt actgagtctc taaaagattt tggaataaat
     3241 gtaggtctat taataggtag tcttacaaaa aaacaaaagg atacagtatt ggaacaaata
     3301 aagaataatg agattgatat attaataggt acacatgcac taattgaaga taaggttgaa
     3361 tttaataata ttggtttagt tataacagat gaacaacata gatttggtgt tatgcaaagg
     3421 agcaaacttt cattgaaagg agcaaatcct gatatattag taatgacagc aacaccaata
     3481 ccaagaactt tagcacttat actctatgga gatttagata tttctattat agatgaactt
     3541 cctccaggaa gacagccaat agaaacaatt gctattgaaa aaagtaagcg agatagagca
     3601 tataataacc ttgtaagaag agaggttgaa tcaggaagac aagtgtacat tgtatgtccc
     3661 ttggtagaag aaagtgaagc catagaagcc aaatcagcag ttgaacttgt tgaggagtta
     3721 agagcggaat attttcacga cttaaggctt gggttgcttc atggtaaaat gaagtcaagt
     3781 gaaaaagatg aagtaatgag actttttaaa gataaagaaa tagatatttt agtttcaaca
     3841 acagtaatag aagttggtgt caatgtgcca aatgcaacat taatgataat agaaaatgca
     3901 gaaagatttg gacttgcaca attacatcag cttagagggc gagttggaag aggtagtcat
     3961 aaatcgtatt gtgtactaat ttatgattct aaaacagatg tatgtagaca aagaatggct
     4021 ataatggaag agaccaatga tggttttaag atttcagaga aggatttaga aataagagga
     4081 cctggggaat tttttgggac aaggcagcat ggtcttccag aacttaaggt agcaaactta
     4141 tttaagcata taaaaatact gaagttagca cagcaagaag ctcgatatat attgggtgaa
     4201 gataataatc tacaattaaa agaaaacatg gcactcaaaa aagaaattat agataaattt
     4261 aaagatacat tgaaagaaat ttcattaaat taattttaaa ttgtgttaaa atattactaa
     4321 tgtaataatg tgctgaaggg agattgtcat attgagagta atttcaggaa aagcgagagg
     4381 attaaaacta aatactccaa aaaatgaaga tgtaaggcca actacagata gagtaaaaga
     4441 gtctctattt aatatgataa attcatatat aatggaaagt gaagttctgg atttatttgc
     4501 tgggactggt tctttaggaa tagagtgctt atctaggggt gccaagtcat gtacttttgt
     4561 agatatcagt aaggaaagca tagatattgt taagtccaat ataaaaaagg caagagttga
     4621 aagtgaaagt attattttaa accttgattt taagactgca atagataaac ttaaattaca
     4681 aaatagtaaa tttgacataa tttttatgga ccctccgtat tataaaaata tgtttataga
     4741 agctattgaa aaaattgata attctaatct attgaacgaa gatggaataa tagttgtaga
     4801 acatgatacg aatgatttat ttccagataa gatatgtaaa ttggagaaaa ctagagataa
     4861 gaaatatgga aatactacat taacttttta taagatggag gcataatatg gaaaataaac
     4921 ctagaaaagc tatatttgca ggaagttttg acccaattac taatggacat ttagatatta
     4981 tttgcagggc ttcgaagtta tttgacgaat tacaaatagg agtattaaat aatcctaata
     5041 aaaagggtct atttagcttt gatgaaagag taaaacttat agaaaaaagt acaagccatt
     5101 taaataatat aaaagttgtg acttttgatg gtttattaat aaattactgt caggaaaatg
     5161 ggattggagc attagttaga ggtgtgagaa gtggtgctga tgttgattat gaacttcaga
     5221 tggcgcatat gaatagagag cttaatccag atatagagac tataatttta ccaagttgta
     5281 caaaatattc atttataagt tcatctttaa taaaagaggt tttgctcttt gatgctgata
     5341 taaaaaattt ggttccaaag attgttttgg aagaattaaa gaaaaaaact agtgggggta
     5401 attgatatat gaagatagat ttagaaatga ttgaactatt tgagcagtta gaggagatat
     5461 ttagaagtgc ttctacaatc cctttctcac ataaatgcac agtggataaa gatgaggttt
     5521 tagcaataat taatgatata agaactttaa taccagaaga agtaacacag gcagtatgga
     5581 ttaataaaga aagaaataaa ataataagtc aagctaagca agatgctaca aatatagttg
     5641 aacaagcgaa aaaagaagct gagagaatac aacaagaata tcaagataat atagaggaat
     5701 taaagaaaaa ttctgaagat gtagtaaagg catatgtaga atccagtgaa cctgttgttc
     5761 aagcagacca aagagcaaaa gatatagttg atagagctga gagaatagcc aatgagataa
     5821 gaattgggtc aattgaatat gcagaggatg tgctttctag tgttgagtat aatttaagag
     5881 aaatattaga agaagtaaaa agagataaag ctgaacttag acctaaaaaa taaattttaa
     5941 ataaataaaa atggagctat cacacaaaaa attccaattt atgtgtgata gctctctttt
     6001 aatatagcaa ttttttgtta tataattgtg ctttttagat tacttatatc ttattttgat
     6061 attataatgt agtttaatct tttatttgtt gaaatcaact tatttatttg tataaataat
     6121 ataggttatt ttcaaatttt tttaataatt ttaccactgt cttcttttag attagagtta
     6181 gactgtgcaa aattataaaa taatactaaa tcatgtgcaa ttatttggta tggcactaca
     6241 tgtagaaaat atagataata aaattaagga ggtagtatga aggaaagggc taaaaaaata
     6301 ttagatttta ttattaaaaa taacaatgta acatcacaag aactacaaga aaagtttaat
     6361 gtttcaaaga gaactattta ttacgatatt ctagctataa atgaacaact aggaaaaagt
     6421 ggtaatataa aaaatgtaaa acacaaattt atatttgaag gtaatttatg tgatgcaagg
     6481 aaaataattt caactgaaga agataaattt ttagatagtg attacagaaa aacttttata
     6541 ttaaacaaga tattacttgg cgaaaaaata tcaatagaaa aactaacaaa tgaaatgtta
     6601 ctttcaaaaa acacagtagt acaaacaata actgatgtaa aaaaatacct tcaaacaatg
     6661 gggttaagat tagagtataa gggaaagtac aaaattatag gtgatgaata cgttattaga
     6721 gagctgtttc tgataattgt tcaagaaaat gtactggaaa ttaattctat aagtgaggaa
     6781 gtaagtagtt tcgatacaaa aggacatata aaacttactg attattcttt attaaatcta
     6841 actaagtttg tggaatttct aaataaaagg ataagagatg ggaaaacatt atactcatat
     6901 aaatatctaa atgaagctaa aaaaattagt tactttagta attgtaaaga acttttatgt
     6961 gaagaagcta atgaaaatga acaagcctat atatgcactt acataagttc acttcccagc
     7021 ttaaatagtg aagttaagga agatgttgtt gaagaatatg tagataaact tatagataag
     7081 tttgaagtaa atacagcaat aaaattagaa agtaagcatg agtttaagaa aaatatttta
     7141 aggcacctcc actcttctta caacagaata cgatttaagt ttcctattag aaatccaatg
     7201 ttagatgaga ctaagtataa acatgagtca ctttataaaa ttataaaatc aattatagag
     7261 aatgaagaag agtttcctgt atttgagggg ataagagaag aagaaatagg atttatagca
     7321 gcgtattttg gaggatattt aagagggtct agggataatg gtctaagaag aaataaagtc
     7381 ttactagttt gtcctaatgg tcttatggta tcaaagagtt tggaaataca actatataaa
     7441 tacataccaa ccatagaaat agtaggtata gtatccatta agcaactaaa agaagtaaat
     7501 gtatactacg attacataat tacaactata gatatacaaa atgtaaacaa cgtaattgtt
     7561 gtaaatcctc ttttaacatc atcagatgtt caattactta tgaacaaact aataagtgta
     7621 aaagaaaacg agaagtattt caatcttgaa ttaataatac aagctataag aaaaaatgga
     7681 gttatcaata atgaagaagc actaaaagct gatttattaa acataataca caagatagat
     7741 gaaggagaga tgtatcaacc aatgttaaag gaattaatta atgcagaaag ggtcaatata
     7801 ataaaaaatg ttagagattg gaaagaagca ataaaaatag cctcaaagcc attgttagaa
     7861 gataactcta tagaagagtt atatatagaa aatatgataa agtcagttga gaaatatgga
     7921 ccatatattg ttttagctga tagatttgct ctaccacatg caagttctaa agaaggagta
     7981 aacaagcttg ctatgtcttt actaatagta gaagatgaag ttgatttatt aggaaaacca
     8041 gtaaatatat ttatggtatt agctgctgta gataacacta ctcacattag agcattagca
     8101 tcactatcag agatgatgta cgaggaagaa aatgtaaaac taataattaa tggtgataaa
     8161 agttcaataa tagaacttat aaataagcaa aattaggggg aatgaaaaat gaagatatta
     8221 gcagtatgtg gttttggtgt aggttcatca atggtattaa aaatgacaat ccaaaaggct
     8281 ctaaagcagt taggtataga atgtgaagta gaaaatacag atataaattc agctagaggt
     8341 gctgattgtg actgtatatt cacaagtcaa gaattgttaa atgaattaaa aggaacttgt
     8401 aatgtagatg tatatccagt taaaaagtac atggatttag cagaagtcac agaagttgtt
     8461 caaacttttt taagtgataa aaatgataag aaatagtata agttttaaaa agtaaattta
     8521 agttaatcag aataaaataa aaacttacta agttcaaaaa tagtaagaaa gagaggaggg
     8581 atttagtggt agaaaactta aacagttgac ctatcactaa aaaatataat gcttgattaa
     8641 aaaatagcta cgcacactcg tgac
//
LOCUS       DAMJWU010000125         8647 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMJWU010000125 DAMJWU010000000
VERSION     DAMJWU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.130"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            16..2193
                     /locus_tag="PC460_003238"
     CDS             16..2193
                     /locus_tag="PC460_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2602096.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFTNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2207..2629
                     /locus_tag="PC460_003239"
     CDS             2207..2629
                     /locus_tag="PC460_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2602097.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3037..3840
                     /locus_tag="PC460_003240"
     CDS             3037..3840
                     /locus_tag="PC460_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2602098.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            3833..4501
                     /locus_tag="PC460_003241"
     CDS             3833..4501
                     /locus_tag="PC460_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2602099.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            4737..5399
                     /locus_tag="PC460_003242"
     CDS             4737..5399
                     /locus_tag="PC460_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2602100.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5459..6256
                     /locus_tag="PC460_003243"
     CDS             5459..6256
                     /locus_tag="PC460_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2602101.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6374..7564
                     /locus_tag="PC460_003244"
     CDS             6374..7564
                     /locus_tag="PC460_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2602102.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            7673..8422
                     /locus_tag="PC460_003245"
     CDS             7673..8422
                     /locus_tag="PC460_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2602103.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3435 a          866 c         1591 g         2755 t
ORIGIN      
        1 atttgaataa aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa
       61 aatgttaata tggatagtta tagacaactt aaagctaaaa taccctgtaa ggaaacaggt
      121 atagaaataa agaggacttt atgtgatatt tgttctccag gagtacattg tggaattgat
      181 gcatatgtta aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat
      241 aaagggttgt tatgtaccaa aggtctttgc aatagagaat atatctatag agaagataga
      301 ataaaaacac ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca
      361 tgggatgaag cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga
      421 gcaaagcatg tagctttttt tacgggttat ggaaagtggt atagaccttt tttacaaagg
      481 tttgcatctt catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca
      541 gcacagatgg ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat
      601 gcaaaaactt atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg
      661 aatctaataa aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca
      721 ccgacaactc aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct
      781 ttagctcttg gaatggcgaa tctatttata caaaaggatt taatagatag agaatacata
      841 gaaaagcatg tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat
      901 actgtaagta gaattacagg agtttctaaa gataatatag taaaagctgt cgagatgttg
      961 tcacaaggtc cattttgtat aaatgaaagt gcagctccaa tagttcacca tcaaaatgga
     1021 tttcaaaatt atagagcaat gatgtcttta tccgctataa caggtaatta tgatagaata
     1081 ggtggaaaca taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta
     1141 gaaaaagagt ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa
     1201 agatttcctc tatggaatga aatggtgact caatttcaag ctatggattt acctaggcaa
     1261 atagaagaaa agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga
     1321 atgtttacta attcagggag actttttaaa gcgttgaata gtgtagattt ttttgtgaat
     1381 atagatttat ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcatgtaca
     1441 tcatttgaga gagaagaatg taagagttat ccaggagggt atattacata taccaaaaaa
     1501 gttattgata aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat
     1561 gctatgaata tagatgatga tttattaaaa gcaggttatg aagcaagtat aagacatatg
     1621 tttaaaaata catgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt
     1681 aaagatttta agccatatat agtaggttca tatacaagag aagggtatga tactagcact
     1741 tctaggtttg aattaaaatc tacaattatt gaaaaatata aagattttgg gctggatgca
     1801 ctaccaacat atagaggatt taatgataat atagatgatg aatatactca tttattgaca
     1861 tcaggagcaa gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga
     1921 atattaaaaa atgaagctag ttgtgatatt tctagcttag atgcaaaaga attgggtgtt
     1981 atcagtggag attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat
     2041 gttactgata gagtcttata taagaatata catatgtatc atggatatag agaagctgat
     2101 ataaatagta ttatttgtga taattttgac ccttatagtg gattccctgc gtatagaagt
     2161 tgtaagtgta agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt
     2221 taaatttaat aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca
     2281 aaatgataat aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg
     2341 tgaaaatata gaatattttt cattagcatg tatacattgt aatgagccaa aatgtttagg
     2401 tgtatgtcct aaaaattgtt ttaaaaaaga agaaggattc attgtattag acaatcaaaa
     2461 ctgtataggg tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa
     2521 tgatggtaag gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc
     2581 accttgtgta atggtatgtc ctacaggagc attaaaattt aataaataaa ttaaatatag
     2641 agaatgactt atacttaggt ataagatatt tttacaaaaa gctgtattga gtttgttata
     2701 atgaaaactc aatatagctt ttttatggat atagttggat actataatgt gattcttagt
     2761 aatagttttt tgctattgga caaagactgt aaaacatatt aaacttatgt tgttcaaagt
     2821 cgaagtataa tataataact agaaaattgg tttatagtgc tataaaagca aatttagtaa
     2881 ggtataaaat cagaggcaat cctttactat aatatcaata tttgggagtt ctgttattgc
     2941 agagctatta aacaattata atggtacaag tgattcaacc tttgaaaatg tattatataa
     3001 aaaattaaaa taagattaaa aaggagagaa taaattatga aaaaacttaa attaattatt
     3061 atgtttgcct tggtatcagc actgttagtt acaggatgtt catcaggaaa taataaaaat
     3121 aaagaagaac aaaaagttat aagagttgca acatcaggaa catattaccc ttttgctttt
     3181 aaagataaag ataaacttaa agggtttgaa gtagattttt gggatgaatt tgctaaacga
     3241 actaattgta aagttgaatg ggtattaact gattttagtg gattatttgg attattagaa
     3301 gctgataaag cagatgttgt atctgctcaa cttactgcaa cacctgaaag agagaaaaaa
     3361 tatgcttttt cagatgctta taattatagt ggaacaaaca ttattgttag agaagatgat
     3421 actagtataa aatcaataga tgatttagtt ggcaagaaag taggtgtggg tactggagca
     3481 gtagcaaatg aaatattaaa agaaaaatac ccaaatgatg aaataaaaat agtaaattat
     3541 tcaagtgcga ctcttaaagg gaattatcaa gacctagaaa tgggaagatt agatgcagta
     3601 gttgcgcagg atgttgaggc tttaatagct ataaaagaac aaaaattaaa tcttaaaatg
     3661 atagaacctc caattcaatt tggtgcctgt tcttttgcgc ttcaaaaaaa tgaaaaaggt
     3721 gaaaaatgga caaaagaaat taacgaagta attgaagaga tgtataaaga tgggactttg
     3781 actaaaatat ctgaaaactg gctaggaaaa gacattacta aggagcctat taatgagtaa
     3841 ttttaatttt gaattttgtc taagtatatt tccatttttg attaaagcat tgggaataac
     3901 aatactaata tctattgttg catttttttt atcaatatta ttaggtgtaa tacttgcctt
     3961 tttcacacag acaaaatcaa aagttttaca atacataaca agagtgtatg tttcattctt
     4021 tagaggaaca ccacttttaa ttcaaatatt tttcttttat tttggatttc caatgatatt
     4081 ttcttttatg aaagattgtt caccttattt agcaatagta atttgtttag gtgtaaattc
     4141 atctgcatat atggctgaaa ttatacgagc atctattgag agtgttgata aaggacaaca
     4201 ggaagcatgt tatgcttttg ggttaagtaa atatcagtca atggttaaaa taattctacc
     4261 tcaagcagct acatctgcag tacctcctat tgtaagctgt acactggaca taattaaagg
     4321 ttcttcttta ggtttaacta taggaataca agatttaatg ggagtagctc aagtagaggc
     4381 tgcttcaagt tttaaatatt ttgagatata tatgataacg atattaattt actgggcagt
     4441 atctataata ttgagtaata tacaaagcaa agttgaaaaa agaataagaa gagcatatta
     4501 gatttcaata tttaaaaatg ggatgatata gatatattat tacttgtaaa taaattttta
     4561 attttagaaa aataaactta gtaatctaaa ttattgagaa aatatagtat caaaatggac
     4621 tgtataatca agttaaaaaa tagtttttct attgaaaaaa taaataaaat tctattgtaa
     4681 tttaatatgc gataaagttt tataaaaaac aacattaagc tagggggact tattttatgg
     4741 cttttaatat gaaatatttt ttagaattat ttccaacaat aatgaaatcg ttcaatgtaa
     4801 ctgtgatttt ggcaataagt tcattagtat tttcattaat tataggtaca atagtagcgt
     4861 tatcatctta ttacaaagtg aaagtattga atcaaatatg taaagtatat gtatctattt
     4921 ttagaggtac acctcttatg ccacagttat tctttttata ttttggacta gcatatatga
     4981 gtgattttgt taaaaatatg gacccagtat ttgcaacatc tatagtactt agtttaaata
     5041 tgggagctta tatgtctgaa acgataaggg catctatact ttcaattgat acaggtcaga
     5101 tagaagcagc atatgcaatg ggattaacaa atttacaaac aatgaaaagg atagtgatac
     5161 cacaggccgt aagagttgca ctgccatcat tattcaataa ttttatagat ttaattaaag
     5221 ggtcttctgt agcttttgtt gttggagtaa atgatataat gggtgctgcc aaatcacaag
     5281 gagcgttgag ttttaagttt tttgaagtgt atggagcagt tatggtaatt tactggtgta
     5341 taataactcc tttaggttgg gggcaaaaaa aattagaaaa gaaaataagc aattattaag
     5401 ttattaagaa aatacataaa agtattttta tataaaaaat ggtgtgagga gaagagatat
     5461 gataaaaaga aatattataa aatgtttagt ggcagtttta tcgttaacgt ttgtaattac
     5521 tggttgttca aaaaaagagg aagtaaagaa tgaaaaagtt ataactgtag cttcatctaa
     5581 ttcagctgac ccatattctt atgttgataa tggtgagcat aagggttttg aagttgacat
     5641 gtggaaagag attggtaaaa gaagtggata taagataaaa atggaaccaa caggttttag
     5701 ttctatattt ggtatgttag attctggtaa agttgatgtg gcagccaact tctttggaat
     5761 gtctaaagaa agattagaaa aatacgatgc ttctatacct tatggttctg attctgtgtg
     5821 tatagctgta aaagatggaa ataacgaaat taataagctt gaagatttaa agggaaaagt
     5881 tgtagcagtt tcagaaggtt ctcaaggtca acaggttgca actagtgtta ataaagataa
     5941 tttgtttcaa gaaaaggttt acggtgatgg aactagtggt cttcaagatt tagatttggg
     6001 aagagttagt gcatggattg atgcagagct tactattgtg ggagacgcta aaaaagctaa
     6061 tatgaaagta agagtattag ataaaaaatt atcagctact aatatagcat acttctttaa
     6121 gaaaaacgat gataaatcta aaacgataaa agaagatgta aacaaagcaa tagaagaaat
     6181 gcttgcagat ggaacagtga aaaaaatatc tgaaaaatgg tttggaatgg atgtaagtgc
     6241 tgatatacaa aaataaatac tttaaaatta aaatgtaaaa ctgtgataac tatagataag
     6301 tatactaaat ttatctatag ttatctgtgt attacaatgt atttagatta gtctttataa
     6361 atcaaaatga gttatggagg tattgcaaat gattgaaact aaaataaaat ccattgcact
     6421 aaaaaataga gaaaaaatta taaaaataag acgacagata cacagtaatc cagagttagc
     6481 atttaaagaa tataaaacat ctaaacttat aaaagaagag ctaaataaac ttaatataga
     6541 atacataaat attgctggta caggagtatt agctactata aaaggaaaaa ataatggtgg
     6601 aaaaactata ttactcagag cggatatgga tgctcttcca atcaaagagg aaaatgattt
     6661 agaatttaaa tcaataaatg ataatatgca tgcttgtggg catgatgcac atgtttcatg
     6721 gttgttagga acagcaatga ttttaaatga cataaaagaa gaattgaatg gaaatgtaaa
     6781 attactattt caacctggcg aagaaaaagg tggttctgat attgtaatta aagaaaatgt
     6841 acttgaaggt gtggatgcat tagcgactgg tcattgttgg ccaactattg agtcaggaaa
     6901 aattggaatc gctagaaatt gtgctatggc agcgactaat acttttgaaa taacaataat
     6961 tggcaagggt gggcatggag cagagcctca taattgtata gacccaattg cagttggaaa
     7021 tgcagtatat tcatccattc aacagatagt ttcaagaaaa attgacccag ttgtacctgt
     7081 agtagtgtct gtttgttctt ttaattctgg tgtttctaaa aatataatac cagatgtatg
     7141 tacacttcaa gggactatta gagcgataag caaggaacaa gtaattgaag taagtaaaat
     7201 tctagaatat atagtaaaaa atgtatgtaa agcacatgga gcagattgca aatttgaaaa
     7261 gagtacagga ggagatgctg ttataaatga caaaaacatg atagagatag gaagagaaag
     7321 tgcttgcaaa atacttggag ctgaaaatgt tgaaataatt gattttcctg caatgacagg
     7381 tgaggatttt gctatttata tgaaagaaaa acctggttta tttatgtata taggtgtagg
     7441 agataaagaa aaaaatataa attacagatt gcatagtaac aagtttaata tagatgaaaa
     7501 atgtcttagt atagcacctt cattattttc acaacttaca gtagattatt tatgtcaact
     7561 atagagtaca ttcataaaaa tatatttagc ctatttttaa atttaatgct agtatattaa
     7621 ttttgatgta gaacatatta tttattatat ttatttaaaa aggtggataa caatgataaa
     7681 agtagaaaat ttacataaaa gttttaaagg gacagaagtt cttaaaggta taaatttaga
     7741 gataagtcaa agtgaagtag tagctatact tggatcatct ggaacaggta aatctacgtt
     7801 acttagatgt ttaaattatt tagttgagcc tacaaaagga aagataatga ttggtgatgt
     7861 aaaagtagat gtagaaaaag caacaaaaaa agatataacc aatttaagaa aacatacatc
     7921 tatggtgttt caaaattata acttatttaa aaataaaaca gcattagaaa atgtaatgga
     7981 ggcattgata gtagttcata aaaagagtaa gaaagaagct agagaaatat ctttaaaatt
     8041 aatagataaa gtaggtatgt tggataggaa agatttttat ccatcaaagt tgtctggggg
     8101 acagcaacaa aggataggta tagcgagagc gatggcagta aatccaaatg taatattatt
     8161 tgatgagcct acatctgcac ttgacccaga acttgttgga gaagttttaa gcgttataaa
     8221 atctcttgca aaagaaggta taacgatgct tattgtaact cacgaaataa attttgcaaa
     8281 agaagttgct gataaaatta tatttatgga taatggtgta atagctgaag aaggaactcc
     8341 tgatgaaata tttaataatc ctaaaaatga aagaacagca aaatttttaa atatcattag
     8401 gaaagaagaa gtatttagtt aaaagtgtat aaattaagca ttagaagata tgtaagatac
     8461 ccccattaaa tttataagtt agataataaa tttaatggga gtatattttt attattagat
     8521 ttattattta atttaaaatt agaaatgtaa ctatttgaaa attcttttta aaagtatatc
     8581 aaaatatcaa atacataaaa aaataatatt tgacaaagta tcatatgtgt ttacaaaatc
     8641 aacttta
//
LOCUS       DAMJWU010000126         8558 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMJWU010000126 DAMJWU010000000
VERSION     DAMJWU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.131"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(3..899)
                     /locus_tag="PC460_003246"
     CDS             complement(3..899)
                     /locus_tag="PC460_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2602104.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1040..2467
                     /locus_tag="PC460_003247"
     CDS             1040..2467
                     /locus_tag="PC460_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2602105.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIAKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFNWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2663..5257)
                     /gene="clpB"
                     /locus_tag="PC460_003248"
     CDS             complement(2663..5257)
                     /gene="clpB"
                     /locus_tag="PC460_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF2602106.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5294..5890)
                     /locus_tag="PC460_003249"
     CDS             complement(5294..5890)
                     /locus_tag="PC460_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602107.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(5907..6311)
                     /locus_tag="PC460_003250"
     CDS             complement(5907..6311)
                     /locus_tag="PC460_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2602108.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            6678..7052
                     /locus_tag="PC460_003251"
     CDS             6678..7052
                     /locus_tag="PC460_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2602109.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7049..7912
                     /locus_tag="PC460_003252"
     CDS             7049..7912
                     /locus_tag="PC460_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602110.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            7912..8550
                     /locus_tag="PC460_003253"
     CDS             7912..8550
                     /locus_tag="PC460_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2602111.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         2866 a         1269 c          999 g         3424 t
ORIGIN      
        1 aattatttca tatcttcttt taaaataaca ttactaactt ctctatgttc atttctatac
       61 ttagttggag atatattata attcttttta aataagcgac taaaataatt tatgctatca
      121 aagccacatc tcatagcaat ttctgtcata tttaaatctg tttcctttaa ataatatata
      181 gctttatcaa gcctgattcc atttataaaa tcgattgtag tcttacctgt tatttgctta
      241 aatgttctac aaaagtgatg tgcactcaag ttcacacgac tagcaagttc ttttaatgta
      301 attttatcca tatatttttc ttctatcaaa tcaaagatac attcaaattt ttctaaagaa
      361 tactttcttt ttataaattc atcttcagaa tatattttat caatatgact tcttaataag
      421 agtactatta attgatagat attagctttt atagataact catatccaac ttctttattg
      481 tgatattcat caagtatagt tgtaatacat tttaaaatca gagaatcatt ttgaattaaa
      541 ttattaaata caatatgatt taaagccaat ggtgctaagt acttggtttg taacaaatca
      601 acttgattac taaaaaggaa tgtgggttca atacgaatac tatagaattt taaattatct
      661 gataagcttt ctaaataatg taattcatta ctgtttgcta ttacaatatt tccaggcact
      721 accttaaaat tatccaatcc acattcaaga tatgctatac catcaataaa ataatatatt
      781 tgtagatgct catgccaatg tcttttaaag tttgctcctt ttttaatgtc atcctttaaa
      841 aaaatatcta taaagttttc ttgacctttc ctgttaaaat atgtattttt atccttcatt
      901 attcccccct catcaaaata gtgctaatta taagcaatat agttattgta ttcattattt
      961 catatcgcta ttatataaaa tgtaactatc attgtaaaca ttaacataat gcagtcatta
     1021 taataaggag gtaataaaaa tgacaaactc ttttaaaaaa gatgataata gaaaaaaaat
     1081 cacagtttta tttgtagtca ttgccatgac ttttatggca acattagatt ctagtattat
     1141 aaatgttgct ctcccagttc ttgcaagcaa actaaatgta tctctagcat ctattgaatg
     1201 ggtaatagca agttactcaa ttattatttg ttcaacacta ttattttttg gaagacttgg
     1261 agatattatt gctaaatcaa gagtatttca agtaggtact attctcttta catcagcttc
     1321 attactatgt ggattaagta attctttaac tttacttata gtttgccgct ttattcaagg
     1381 aataggcgct tctgcctaca tggcaaacaa tcatggaatt atcacagaac tatttccaaa
     1441 agaaagcaga ggaaaagccc tagggatttt agttactgca gttgctattg gaaatatggt
     1501 tggaccatct gtgggaggat ttattttgtc aatatttaat tggaatgtta tattttttat
     1561 aaatattcca atcggtctta ttgtaatctt tttaaataca aaatttcttc ctaatagtaa
     1621 aaaatcttca gaaaatatgg ataaaactgg tgctatactt caatttttag gaacaacatt
     1681 attttttagt gctctaatta gtgctcaaca aacaggatta ctaaatccat atatattaat
     1741 tgcactatta ttatcaatta tttttattat attatttttg atattagaaa aaaaacatcc
     1801 tcaaccgctt cttgaccttg aaatttttag aaattttaaa ttttcattga atttaatttg
     1861 tgctcttaca tcattcattt gtattgcatc atcaagtatt ttaattccat tttatttaca
     1921 aagtactatg aaacttcccc ctattcaagc aggtttattt atgatactat cacctttgat
     1981 tttagccatt ttttcaccta tatttgggaa tatatcagat aaaattaagt cagaaaaaat
     2041 tattttaatt ggtcttctcg ttatgagttt tggattcttt ctaatgtcaa gattgaaaga
     2101 aagttctgcc cttattttat ttgtaattta tatatcgata atatcaatag gacaagctat
     2161 atttcaacct gctaacaatg ctttgataat gtcaagctgt tctagaagta agctaggagt
     2221 tgtaggaagc ataaattccc ttgtaagaaa tttaggacaa gttataggta ttactatttc
     2281 tacaacactt ttatataatt ttatgagtat taaagctgga tatagggtca atgattatgt
     2341 gattaataat gataaaatat ttgtatttgg aatgagaaat gtttatatta ttgttacact
     2401 tgtttgctta ataggtgcaa tattaattgg cttttattta tttaaatata ataaaaatga
     2461 acaatagttt ttataattat attcaaatta tgattatatt ataagaaatt tactaaaata
     2521 cttcctttga gttaaattta atttggacat attgacttaa aaatgtttta ttaatttgat
     2581 aaaatatttc ttttcctata gtttataaat aaaaatcagc tgagcaaaat atttagtata
     2641 ttatgctcaa ctgattcttt tattatttaa ctaaaacttc aatattttca tcttttccat
     2701 ctataactat agtacatcca ttatacacat ctccagcaat aagtttcttt gcaattatag
     2761 tttctaatgt atttcctata tatctcttaa gaggtcttgc tccatataca gggtcatatc
     2821 cttctctcac cataacatct tttgctgaat ttgtaacttc aatagtaata tctttttctt
     2881 ttaatctatt ttttaaatct ttcataaata tatctataat tttcttgata ttttcttggt
     2941 ctaaaggctt aaacattata atatcatcta ctctgtttaa aaactcaggt ttaaatctat
     3001 gcttcatttc attcattact aaattgttag tagtctctgt tatatttcct cctgcttcta
     3061 atagataatt acttcctata ttagaagtca ttattataag agtattttta aagtctactg
     3121 tcttaccttt attatcagta agtctaccat catctaatat ctgtaagaac atgttaaata
     3181 catcctcatg tgctttttct atctcatcaa atagtattac agaataagga gcacgtctaa
     3241 ctgcttctgt aagttgacca ccctcttcat atcccacgta ccctggagga ggtccaacta
     3301 gtcttgatac agcatgtttt tccatgtatt cagacatatc tattcttatt atattatctt
     3361 cactatcgaa taaatttcta gcaagagttt ttgcaagttc agttttacca acacctgtag
     3421 ggcctaagaa aataaatgaa ccaattggtt ttctttcatc ttttaatcct gctcttgcac
     3481 gtattactgc atttgaaact gctgtaactg cttcatcttg tccaataact cttttatgaa
     3541 gttcatcttc tagtttaagt aatttttctc tttctccttc aactagttta gttactggta
     3601 tacctgtcca ctttgataca atgctagata tttcttcttc agtaacttct tcttttaaaa
     3661 gagaattttc agaaccatca ttcatatttt cttcgtactg ctttatttgt tcttctaact
     3721 taggtatttc accatattta acctctgctg ctttgttgaa atcatattct ctttcatact
     3781 tttcagcttt acctttagct tcgtctaatt gagcctttaa gtttttaata tctaatattt
     3841 gactcttttc tttttcatat tttgctgtca tttcatcatt tttagatttt aactctgcta
     3901 tttctttttg aatatcatcc aatcttgatt tacttttatc atcattttct ttaagtaatg
     3961 cttctctttc agtttctaat gtaaataact ttcttctaac aacatctaat tctgttggca
     4021 atgaatctat ttcactacga atcatagctc cagcttcatc aattaaatca attgctttat
     4081 ctggtaaaaa tctatcttgt atatatctat gagaaagttt tgctgctgct acaatagcat
     4141 tatcatgtat tcttactcca tgatgtattt caaatctttc ttttaaacct ctaagtatag
     4201 atattgtatc ttcaacagta ggttcttctg caattacagg ctgaaaacgt ctttcaagag
     4261 ccttatcttt ttctatatac tttctatatt catcaaaagt agttgcacca atacagttca
     4321 attcacctct tgcaagcata ggtttaataa gattacctgc atccattgaa ccttctgttt
     4381 taccagctcc aacaatagta tgaatctcat ctataaacag tataattttt ccatcagaac
     4441 tttgaacttc ttttaaaact gcctttaatc tttcttcaaa ttcacctcta tattttgcac
     4501 cagcaattaa agctcccata tcaagtgaga atacaatctt atcttttaaa ccttctggga
     4561 catctcctct tactattctc tctgctaacc cttctacaat tgcagtttta cctacacctg
     4621 gctcaccaat caatacagga ttattttttg ttcttcttga taaaattctt ataatccttc
     4681 ttatttcttc atctctccca attacagggt ctaatttatt tttcttggct aaatctacta
     4741 agtttgttcc atatctagca agtgcatcat atgtgccttc tgggtcttgt gtttctactc
     4801 tttgactacc tctcacttgt gataaaatat ttaaaaagtc attcttattt atattatatt
     4861 gttttaatat tttaccaaca gctgattttg attctgtttc catcattgca agcataacat
     4921 gttctacact tatatatgaa tctttaaatt ctttagaaat actctctgcc tttataagca
     4981 cctcatttat ttttctagta gctgatactc cttgtgagtc agcaccttct ccatatactt
     5041 ttggaagctt atcaatttca aagtttacag tatttctcaa agaatcaatt gatatattca
     5101 ttttttcgat tatatttggt atcaatccat cctcttgatt aataagtgct gagaaaagat
     5161 gaattacatc tacttgttgg ttatgatttt ttactgcttc gttataagcc tcatttaaac
     5221 ttttttgaac tcttaaagtc attttttcta cgtccatata aatccctcca ttttaatatt
     5281 atatccaaat aaattaaatt ctctcattta taattattga ttttctaagc tctaaaagac
     5341 tatcaagtaa attctcgttt ttttgtatat cgaaactcaa tgttaaatta aatgttccat
     5401 ctgaattaaa aaaatcgaat acttcttttg attttgctat taaatacaag tcttcatcta
     5461 taagtctttt ttcacctatt aataaatact gacatttatc tataaaactc tttttaaaat
     5521 caatagtgta tagattttct aaaatattat attgataaat agcaatatta ttaaacactt
     5581 ttaatatgtt tgaatttaca aaattgtaaa tttgattatt gttatacagt gatatttgtt
     5641 cgtatatata ttccctaaat aaaggatgaa tgttttgctt aagttttaaa gtaacttcag
     5701 ctggtatttc aaaataaagc ttccagtact tagagttatt tttttctaaa ataaaagcta
     5761 ctttgttcaa actggtagaa ctaatctcct ttagtgtaca attaaaatta tagtgtctca
     5821 aactagtttt ttcattctca aatacatagt caaaccactg tagaacattt ctatcaagtt
     5881 ttaaactcat ataaaaacct tcttccttat ttactaacct tattaggtct ttttatctga
     5941 ttatattctg ctaataaact ttctttatca gctctactac ctttatatgc tatatgtgga
     6001 tttatcatat actcaccatt tttcactctt tgtaaaaaat ccattttttt cagctccgta
     6061 aacaatgaag ataaagaagt ttttggatat tcaaattcac aagataattc atataatgtt
     6121 tttctaatct tattattttt atccatagag tttattaatt ttaatggaat taaatccctc
     6181 ttagtataca ttccttctac tactttaata taattcttaa aacaaacatt catatactta
     6241 cttttattca taactaaact tttgctctct gtatattcct gatttaaatc cttttttaat
     6301 gtcttatcca aattaaatat ctcttctcac tctccttatc tctaattgat aatataaata
     6361 tattacaaaa aaatatgtat gtcaaaactg aaaatgcaaa aagttcacct tataagtaaa
     6421 cttttattta gctataagct aaactttatt atttttttaa tttagtattt ccaatttatt
     6481 ggcaggattt ttgaacaata tttataaatt ttttaaaaaa atttaaatct atttaatatg
     6541 gcagactttc ataaaataca ttacatttat taaatgtata atattggcaa ttagtttttt
     6601 ctaaaatcag ttgacatatt atatatatat ttatataatg tgtatatact atatatacaa
     6661 tttaaggagg tgtcttattg aatatagtta ttagtaattc gagtgggaag ccaatatatg
     6721 aacagattac tacacaaatt aaaagtatga ttataagtgg agagttacca gaaggctcag
     6781 ctcttccaag tatgagacta ttggcaaaag aacttagaat aagtgtaatt actacaaaac
     6841 gagcatatag cgatttagaa cgagacggat ttattgagac agttactggt aaaggcagtt
     6901 ttgtatctag taaaaacatg gattttgtca aagaagaaca atttcgttta attgaagaat
     6961 acctacaaaa agcagttgat acagcaaaaa gtagtgatat tacatatgac gaattaaaag
     7021 atattttatt tttattatat aaaggagaat gataagtatg gatgcaataa ctataaaaaa
     7081 tttaaataaa acttataaag attttagcct acaagatata tctttttcag taccaaaagg
     7141 gagtgtaatg ggatttgtag gtgaaaatgg tgcaggaaaa acaactacat taaaagcaat
     7201 tttaaattta atatcttatg atagtggaaa tatagaaatc tttggtcttg atagtaaaaa
     7261 aaatgaaaaa gaaattaaag agcaaattgg ggttgtattt gagggaagca actttcatga
     7321 aaatttaaac actgaccatg tttcaaaaat catgagtaaa atctataaaa attggaatga
     7381 cacattattt aaagactact taaaaaaatt gagagtacca gacaataaat taattaagga
     7441 gttttcaaag gggaataaga tgaaacttag tattgctgta gccttatctc ataaaccaaa
     7501 attattaata ctagatgagg caactagttc acttgaccct atcgttcgtg aagaaatact
     7561 agatatattt ttagatttta tacaagatga agaacactct attatcttat cttcacatat
     7621 tacaagtgat ttagataaga tagcagacta tattacattt atacataaag gaaaaattgt
     7681 atttagtgaa aataaggatg aattaattga tactatggga gttttaaaat gtaaacctag
     7741 tgattttgat aacctttcaa gagaggatta ttcatattat cgtaaaagcc aatttggata
     7801 tgaagtactt cttaaagata agcatagatt tatctcaaga catccaaact gtattgttga
     7861 taatacgtct atagaagaaa ttatgttatt ttatgtgaga ggtgataaat aatgaaaggt
     7921 ctaattttaa aagatttact taatttgaaa ggaaatataa agtttatact tttattcatc
     7981 attatgtttg gctttatgtc ttctttagga gatggaaatg ttaataactt tataggtgtt
     8041 attatagttt tatgtactac aatgattgtt tcaacatttt catatgatga tttgaataaa
     8101 tgggatagtt atgtacttac aatgcctatt aatcgcaatg atattgtatt aagtaaatat
     8161 ttaactatgt taatatttag ctttataggt gttcttgttt cacttattgt tagtgtaact
     8221 attggttatt ttaaaaatac acttatatta aatgaaacac tacttattaa tgcactaatc
     8281 ttatcaattt cagtatgttt tggtagcctt attctaccac ttatttataa atttggtaca
     8341 gaaagagctc gtttattaat gattttatgc tttttagttc caacactagc tttattagtt
     8401 tttaaaagta ttttagaaaa tattagttca cctatttcta tagaaattat acttaataca
     8461 ctagtatatt ctcttccatt tgttgctatt ttactatttg tgatttcata ttttatatca
     8521 tcaaaaatct atagtaaaaa agaagtataa aatatact
//
LOCUS       DAMJWU010000127         8456 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMJWU010000127 DAMJWU010000000
VERSION     DAMJWU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.132"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            253..1239
                     /locus_tag="PC460_003254"
     CDS             253..1239
                     /locus_tag="PC460_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2602112.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1408..1556
                     /locus_tag="PC460_003255"
                     /pseudo
     CDS             1408..1556
                     /locus_tag="PC460_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1575..3056
                     /locus_tag="PC460_003256"
     CDS             1575..3056
                     /locus_tag="PC460_003256"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF2602113.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3082..4053
                     /locus_tag="PC460_003257"
     CDS             3082..4053
                     /locus_tag="PC460_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF2602114.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4309..4911)
                     /locus_tag="PC460_003258"
     CDS             complement(4309..4911)
                     /locus_tag="PC460_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2602115.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            5192..6403
                     /locus_tag="PC460_003259"
     CDS             5192..6403
                     /locus_tag="PC460_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF2602116.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6471..6668
                     /locus_tag="PC460_003260"
     CDS             6471..6668
                     /locus_tag="PC460_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF2602117.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6702..7673
                     /locus_tag="PC460_003261"
     CDS             6702..7673
                     /locus_tag="PC460_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2602118.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            7860..8237
                     /locus_tag="PC460_003262"
     CDS             7860..8237
                     /locus_tag="PC460_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2602119.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3442 a          881 c         1359 g         2774 t
ORIGIN      
        1 aatcatagcc ttatttttat attataaata aattagagta tttatcaatt catagtagat
       61 aaaattgaaa ttaaatataa caacaaaaaa tgaaaattac aattaattta atgttaaaga
      121 ctcaattatt tggatgtgga atggatagta caagtagtat ataaaaagta ttgactggac
      181 aaataactat attttatttt aaatatataa tataatgtaa taaaatatat caaatgtaga
      241 aggtgataac ctatgaaaaa aataacaata aatgatattg ctaacttggc aggagtatct
      301 aaaagtactg tatcaagata tctaaacaat aaagatatca gtgattctac taaagagaaa
      361 ataaagacaa taatagatga atatggatat gagccgaatg catttgctca aagtttgaga
      421 gctaaaaaaa catattttat tggtattata acaccgtgtt tagattcttt tgtaaaatct
      481 aaaattatga tggcaataga tgaagaactc aaaaaattaa aatatacatc actaattatt
      541 aatactaata gaaaaataag gtcagaaata gatagtatat caaagttagc tagtctaaaa
      601 gttgatggaa taatactaat tggtaccgaa ataactaaag aacataagaa tgaaattgaa
      661 aagttagata taccgatagt tgtagttgga caaaaagttg atggtatcaa tagcatagtg
      721 aatgatgatt atggagcagg ctataaaatg ggtcaatata ttgctaataa aggttacaaa
      781 aacattgtat atttaggtgt tgatgaaagt gatatatcag ttggtttaaa tagaaaaaat
      841 ggagtactta atgggttaaa agataaaggg tatgatgcaa aagtatttta tactgacttt
      901 gaccaagaaa tgtctataca aagaagtgga gaaatgctag aaagtgaaaa tccagatata
      961 ataatatgtg caactgataa tatagcaata gcaacaatga aagaaatcaa taagagaggt
     1021 aaaagtatac cacaagatat atctgtagct ggatttgggg gatatgatgt attgtctatt
     1081 ataagcccta aacttacaac aataaagttt gaaaataaaa atgcaggtaa agtagcagca
     1141 aatacgatag taaatttaat acaagaaaga aaagaaccac tattaaagga gattaaattt
     1201 gaattaatag aaggtgaaag tacaataaat aaaaattaag atacacatta catatattag
     1261 taatgtgtat ttttttacta aaaaaactat aattctaaaa attaggaaaa cttttttgaa
     1321 agaaatattg catctgaaat aaatttatgc taatctttaa ttaaaccggt ttcgaaaccg
     1381 gtttaattaa accgatagaa tagcaaaatg agtgcaaaaa taactgattg taacaaagta
     1441 tcaaagtaat tattggagta cataggaata aacaaaatat aaaagatgta gcacattgtg
     1501 taataagact aagaattgtt ttagatagca ataataaagc aaatataaaa ttataaaaga
     1561 aaaggaataa aaatatgtac aaagagccta aatatagaac catattagaa tcgacaaaag
     1621 aagaattaaa taaattaaga aatatatctt taaatgataa atataaacca ctatttcata
     1681 tacatccaca acatggacta cttaatgacc caaatggatt agcatattat aatggtaagt
     1741 accatgtatt ttatcaatgg tatccatatg atgcaactca tggaatgaag cattgggctt
     1801 atgtaagttc tgatgatttt gtaaactgga atagagaaga tgtagcatta ataccaatcg
     1861 aaagttatga atctcatgga gcatattcag gtaatgcgat agaggtagat ggaaaattgc
     1921 atatgtatta tacagggaat ataaaatata gtgcagaaga tagatatgca tatcaaaact
     1981 tagctataat gaacaaggat ggtaaaataa ctaaatatga aaataatcca atagtaagtg
     2041 aaataccaaa aggatataca ggacatgtaa gagacccaaa agtatttaaa agaaaggata
     2101 aatactttat gttacttgga gcacaaacaa gtgataaaaa aggagttata atagtatatg
     2161 aatctaaaaa ctctatagat tggaatttta aaggagaatt aaatgtaaaa aacatagatg
     2221 aagattttgg ttacatgtgg gaatgtccag attatattaa tatagatgaa aaagatatat
     2281 taatattttc accacaagga gtagaaccta aaggatttga ttatcaaaat atatataatg
     2341 tagtatatgc tattggtaat atggatttag ataatttaac atttgaaata gatacaatga
     2401 aagaactgga aaaaggattt gatttctatg ctccacaaac atttataaag gactcacaaa
     2461 taatactatt tgcatgggct ggaatgggag aagttttata ccctacagat aaaaacaaat
     2521 gggcacattg tctaactgtt ccaagaaaat taaatataaa aaataataag ctattgcaaa
     2581 tgccagtaga tgaattaatt aaattaagat atgatgaaac aagtgggcaa aatactataa
     2641 agaataatat taatattata gaaaatgatg aaaatgtata tgagcttaat attaatataa
     2701 aaaatattga cagcaataaa tttgggctgg aattattttc atcacaagat gaaggtgtaa
     2761 aattagaatt taacaaatta ggaaacattg taactttaga cagaagcaac tttaaaaaag
     2821 ttttcggtgt tgaatatgga acaaatagaa aagaatatat aaatattgat gaaaatacta
     2881 atatcaaagt actagcggat agaagtattt tagaaatttt tataaatgaa ggagaagtag
     2941 tatttactag tagaatattt gctaaagaaa attctaatca aataagggtt tatagtgata
     3001 aaatagtaga gtatgagtat actaaattta aattaaagca aggtattgaa ttataaaaac
     3061 acattataag gggtagaaaa gatgaaaaaa gtaattagta taggagaagc tttaatagac
     3121 tttataccta atcaaaatgg tggtaaatta aaaaatgtat cagaatttag aagagtagca
     3181 ggaggagctc cagcaaatgt tagtgctgtt gtagcaaagc ttggaggaaa atcgagtttt
     3241 atatctaagt taggaaaaga tgcatttggt gattatataa tagatgtatt aaatgaagta
     3301 aatgtaaata ctgattatgt attgagaaca agtaaagcaa atacaggact tgcttttgta
     3361 tcattaaaag aagatggtaa tcgtgatttc tcattttata gaaatccaag tgcagatatg
     3421 cttttagaag ctgatgaagt taaaaaagag tggtttaata attgtcatat cctacatttt
     3481 tgttcagtag atttaataga tagtccaatg aaattagcac ataaaaaagc aatagaatac
     3541 gcacttgaaa gtaatagtat aataagcttt gaccctaata taagattacc tttatgggat
     3601 agtgagcaat cttgtaaaaa ggcaatatca gaatttttac cttttgctca tattgttaag
     3661 atatctgatg aagaattaga atttataaca ggagaaaatg atatagaaaa atcattacat
     3721 aaactctttg tggggaatgt tgaactagta ttatatacaa aaggaaaaga tggagtagat
     3781 gcatacacta aaaaagtaaa aggtatgtgt aaaggggtta aagtaaatgc aatagataca
     3841 accggagcag gagattctta tataggttca tttttataca ccttattgta taaacaaata
     3901 acattgaatg atataaaaga aatgaaacaa gaaacgttaa atgagtattt agaattttct
     3961 aattattatg cagctcgtag tacaactatt aaaggtgcga taagttcata tgcaacaaag
     4021 gaagaaataa ctgaatttat aaaaaattta tagtaggaat gagtaatagg ttttgaagga
     4081 agtaacatgt aaagagtttt taaggatgtt tactatgaaa ataattaaca tataaaaagc
     4141 aataaggagg ctgtctcaaa atagagataa gcctctttat cattgttgaa atgataaaaa
     4201 atattttaat tattgttact ttttagaaga atatatatct tattttaaat aagcaaatta
     4261 tgtaagattt atttatatta tcaatacttt acttggagta ttatgtcttt agcagttatc
     4321 taattcaatt gaattagata aaatataata aatttgtttt gcaaattgac gagaatgttc
     4381 ttctttttct ttattcataa aattaggttt acctaaatca cgttcaacaa gcattcctat
     4441 atattttaac tgtgaatgct tacagtagcg cttcatacca ttttcccaaa tatcagcacc
     4501 cttttcaggt ctatatccac atgttgtaat aagtgctact gattttcctg cccatagagc
     4561 ggggcctttc tcattaccat agtatttatt cattccatac accaatctgt caagtagtgc
     4621 tttcattgga gaagtgcagt accaagaata aattggagta gcaaaaacaa ttaaatcaca
     4681 cataagaatt ttcttgaaaa ttttctgaac atcatcttta caataacatc caaaatcaaa
     4741 taagttatcc tgacactttt tacaagcaat acatggctct attttacaat catataacca
     4801 tattacttca atatttgaat tataattttc taattcctca ataaaaggtt ttaaaatagc
     4861 agcagtatta ccttttttct taggacttcc cataagaatg catacttcca tattgatacc
     4921 tccacagtat tttaaaatag tttttagtgc ttaatatagt tttaggatac tatgagaata
     4981 cgttaacaac aatacacttt gtatatttgt aataaaattg aaacaaattg ttttatcaat
     5041 aaaagttgga atgtatctat aattaaaaat attttaggta tgtttttaca tatatgtgtg
     5101 tagttaacta tggtaattta ttgtaaaatt atcttatagg attataattg cttaatttat
     5161 gattatattt aaaatcttgg aggtaaaaaa tatgtactca aatgaagact ataaatttat
     5221 agaagaaaat gcaccatgtt atatatatga tgagactaat ataattgaaa aatgtaagga
     5281 attgaaaaat aatattttag gttttgattt tttatattct ataaaaacaa atccatataa
     5341 caaaattgta aaaagcattg cagagcaagg ttttggagca gatgcttctt caactaatga
     5401 agttatgatt tcatcaaaat gtggagtttc taaagatgat atttattatt catcaccagg
     5461 caaaacagag attgatatag aaactgtttt agataaatgc ataattattg cagatagtat
     5521 tacagaaata gaacttataa ataagatatc taaaaagcat aatcagaaag ttaaaatagg
     5581 aataagaata aatcctgatt ttactatgga tgatgatagt gctaaatcaa gcaaatttgg
     5641 tatagatata gaagatatga gcagtgtttt taagatgtta agtatgtgtg attatgtatc
     5701 tgtagttggg attcatatac atattaaatc tcaggtattg gattttgaaa agttaggaca
     5761 ttattatgaa aaatgttttt ttatagcttt aaatttaaat aaaagtaagt atattaatat
     5821 tgaatttata aattttggta gtggacttgg agtagcttat gataatctta gagaaaatcc
     5881 acttaatctt aaagagcttt caaaattaac tcaaaagatt gcttttacta attcaaacac
     5941 actaaaatca agacttctta ttgaatcagg acgtttcata gtttgtaaat caggtaatta
     6001 ttatgttaaa attgtagata ttaaagtatc aagagggaaa aaatatctta ttgtaaaaaa
     6061 tggacttaat ggatttatga gacctattat ttctaattta attgaaaata ttacttgtgg
     6121 agtagaatta gaaggtcaag agccattata tacatcaaat catgcctttt ctctttcagt
     6181 attaaataat ttaaaagaag aagagtatgt aaatgttgtt gggaatctct gtacatcatt
     6241 agatgtaatt gcaaataatg ttaaattgaa aaaagcaaac ataggagatt tgatttcaat
     6301 tagtaatgct ggaagttact cctattcttt atcaccgcta ttattttcaa gttatgaatt
     6361 tcctaaacaa tattataaaa gaaaagatgg atatattgag taaacaatag cataaattaa
     6421 taaacttagt attattgaaa aaatttaaaa tgcagggggt gtaattctat atggaattgt
     6481 ggaaaagaaa tttatttgta tgttggatag gtatgttttt tagttctata gggatgagcc
     6541 aaatagcacc tatacttcca ttatatatta aacagttggg ggtaactgat gttagtttaa
     6601 tacaacagta ttcaggtata atttttggat gtacctttgt agtagcagca ttcttttcgc
     6661 caatttgagg gaaagctgct gataaatatg gaagaaaatc aatgctttta cgtgcaagta
     6721 ttgggatggg aatagttata tttaccatgg cgtttgttca aaatgtttat cagttattag
     6781 gattaagaat acttcaagga gtttttacgg gttatgctac tgcatgtact actcttattg
     6841 caacacagac agataaaaat cattcaggat gggctttggg tactcttgca actgcatcaa
     6901 ctactggctc attaattggt ccaacagtag gaggatatat agaatcaatc ttaggtctta
     6961 aaagtacatt tattataaca ggaggattgt tgtttgtatc atttatcatc tctattttat
     7021 ttgtagtaga taattttaaa cctaaagaaa taaaagaatc aatttctata cctaaagaac
     7081 aattaaatat attgccaaat aaatttttaa ttgcatctat ttttgtaacc acattcatta
     7141 ctcaactagc tttatactct atagaaccaa ttgtaaccat atatatatca caacttacaa
     7201 attttgcatc aaatgtagct ttaatagctg gattgacatt ttctgcatca ggattagcaa
     7261 atttattagc agctcaaaaa cttggaaaaa tgtcggataa aataggtcct caaaaagtat
     7321 tgttaatttc acttttatgg gctgggatta tatttatacc acaagctttt gtaaaaactg
     7381 catggcaact gatgttgttg agatttttat tagggttgtc agttgcaggg cttaatcctt
     7441 ctgttaacag cttgcttaaa aaaattgcac cagaagaata tgtgggaaaa atatttggat
     7501 ataatgcatc tgcccagtat ataggctgtt catcaggagc atttttaggg ggacaaatat
     7561 cagcacattt aggtattaga acagtatttt tttcaacaag tttattgttg tttaggaatg
     7621 cactttggat gtataaattt actggattgt ttattaaaga taagactaaa taatcatact
     7681 atttacattt ctaattttat gatgtaaaat aaatattttt tatgtaattt atcatagaat
     7741 tatataaaaa ataaccctat ggtaaaaatt ggaaaatatt attatcgaca aaatctttga
     7801 cttaaaaact atattgcgat agtattaatt taataaaagt ttgagagggg cgtaaggtta
     7861 tgacaatttc aaaaatacca caagcagaat taaaagttat gaagtttatt tgggaaaaaa
     7921 atgatacagt aacatcaaaa gaaactattg aagctatgga acgaaaatat ggttggaaac
     7981 aaacaactac attgacactt ttatcaagac ttgttaaaaa acatttttta agtgcagaaa
     8041 aaatagatag atacactcat tatacaatag ttgttggaca taaggagtat ttaagtgttg
     8101 agacaaaaga ttttttaagc aatatacacg acaattcttt acagagttta atttcagcac
     8161 tacatgatga tgaagttgtt gataaagata tgttggattt atttgaaagt cattttaaaa
     8221 atttagaaga agagtaatta tatcaggata gaactctaag aatttaatta atctatggct
     8281 gaaaaccata aatggtatta tttaatcagc tactcactat tcataaagta atattaaata
     8341 ggttctctaa ttagtaaaat gcctaaaata attggaatag ggagtatcaa gttaaatgat
     8401 accacttatt taaataagaa atgattttta aaatttaaaa taattttata acttgt
//
LOCUS       DAMJWU010000128         8398 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMJWU010000128 DAMJWU010000000
VERSION     DAMJWU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.133"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            94..849
                     /locus_tag="PC460_003263"
     CDS             94..849
                     /locus_tag="PC460_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2602120.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            980..2002
                     /locus_tag="PC460_003264"
     CDS             980..2002
                     /locus_tag="PC460_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2602121.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2166..2723)
                     /locus_tag="PC460_003265"
     CDS             complement(2166..2723)
                     /locus_tag="PC460_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2602122.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2729..3805)
                     /locus_tag="PC460_003266"
     CDS             complement(2729..3805)
                     /locus_tag="PC460_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF2602123.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3806..4489)
                     /gene="modB"
                     /locus_tag="PC460_003267"
     CDS             complement(3806..4489)
                     /gene="modB"
                     /locus_tag="PC460_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF2602124.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4638..5450)
                     /gene="modA"
                     /locus_tag="PC460_003268"
     CDS             complement(4638..5450)
                     /gene="modA"
                     /locus_tag="PC460_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2602125.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMEDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5643..6572
                     /locus_tag="PC460_003269"
     CDS             5643..6572
                     /locus_tag="PC460_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602126.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6733..7182)
                     /locus_tag="PC460_003270"
     CDS             complement(6733..7182)
                     /locus_tag="PC460_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602127.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7385..8365)
                     /locus_tag="PC460_003271"
     CDS             complement(7385..8365)
                     /locus_tag="PC460_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2602128.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2922 a         1383 c         1012 g         3081 t
ORIGIN      
        1 ctttcctagc tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta
       61 ccaaaaataa atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag
      121 agcttcccta atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata
      181 gaagatggtg aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta
      241 aatataatat ccgggcttat aaaagaatct tctggtcaag tattattaaa taaacataac
      301 ttgtctaatt tagctgaaca cagaagaact caaatagtaa gtagagtttt tcaagaccca
      361 agtcttggaa cttgcccttc tatgactgtt agagaaaact tgtctttagc tcttaacaaa
      421 ggaaaactat taaatctaag aaaatgtctt cgttacaaaa gagatttctt agaaaattta
      481 ttggaaggtg tatctcttga tttaaaaaaa taccttgatg ttcaagtaaa atttttatca
      541 ggaggtcaaa gacaatcatt atcactaatt atgtcgtgct taacaaatcc atcagtttta
      601 ttacttgatg aacatacagc agcacttgac ccaaaaacat caaatgaagt aattgaattg
      661 actaataaga ttgtaaggga aaaaaatata actacactta tggttactca caacttaaaa
      721 cacgctcttc attatggtga tagattgata atgcttcata agggtgaagt tgtattagat
      781 gtaaaaggta aagaaaaaga acaattaaca gttgaagaaa tcttagaaaa atttgaatat
      841 gcagtttaaa ataatatatc tattgtatta aactcggctt taaaataaag ttaaaattac
      901 aataattatg gttactttgt aataattcat atgaatatat gaacatatat aataataaat
      961 ttttgagggg gatataaaaa tgattaataa aaaaagattg gcaagtttga ttttagctgg
     1021 tgctcttagt atatctatgc ttacagggtg ttcacaagga ggagactctg gtaattcaaa
     1081 acaagaaagt aattctaaag gtaaagaagt taaaaaaatt ggtataactc aactggttga
     1141 acatccagca ttagatgcca ctaggacagg ttttgtaaaa gcattagaaa agaacggatt
     1201 taaagatgga gaaaatattg atatagactt ccaaaatgct caaaatgaca tgcctactac
     1261 acaaagtatt gcgagtaaat ttgcatctga taagaaggac ttaatatttg ctatatctac
     1321 tccatcagct caggcagcat ttaatgctac taaagatata cctatactta ttactgctgt
     1381 gagtgaccct gttgctgctg gattagttaa aactcttgaa aagccaggca caaatgtatc
     1441 tggaacatca gattttgttt ctgtagacaa aggtttagaa cttcttaaaa tatttgctcc
     1501 taaggcaaaa actataggag ttatgtacaa tactagtgaa gtaaattcca aagtccaagt
     1561 tgatgcttta aaagaatatg catctaaaaa tggatttaaa gttgttgaaa aaggtataac
     1621 tacttcaaat gaagttaacc aaggtatttc tagtttagtt ggcaaaatag acgtattata
     1681 tgttcctaca gataacttag tggcttcttc tatgcctata gtctctaaaa tagctactga
     1741 gaataaaata cctgttatag cagctgaatc tgggccagtt gaaaaaggtg ctcttgcttg
     1801 tcaaggtatc aactacgaaa aactaggtta caaaacaggt gaaatggcag ttaaaatttt
     1861 aaatggtgaa tcagtatctg atatgcctgt ggctacatca gatgatacag atatcattgt
     1921 taatgaagat atattaaaag ctttaggtat ggaaaaacct tctaatgaaa atatttctta
     1981 tgtaaagact aaacaagaat agaaagatat tttctatata cgattaatat gcctgtttct
     2041 ataaattaat ttatagaaac aggcatatat ttttatttta atgtattatc tatttaaaga
     2101 ttatatgtaa atgctcttaa tttgtttgta taaattttac ttgtataaac tttatacaat
     2161 catatttatt cttctatttt ctttattact ctacatggat ttccaacagc tacagtattt
     2221 ggtggtatat cttttgtgac tacacttcct gcacctatta ctacattatc acctatagta
     2281 attcctggtg ttatgattac tcccccacct atccatacat tatcacctat ttttacagga
     2341 gaaccatact ctatacctga atttctaagt tgtgcatcaa ttggatgata ggctgtatag
     2401 atttgaacgt ttggagctaa cattacattg tcacctattt caattttaca tacatctaaa
     2461 aaaatacaat cataatttgc aaagaaattt tcgcctacat gtatgttata tccataatca
     2521 cacctaatat tttgttctac atttatttgc ttgccaactg aaccaaatag ttgtcttaaa
     2581 atatcttctc ttttttcata ttcatcctct aaagtattat taaacaatct agttaatttt
     2641 ttacaatact ctctttcttt tactaacaat tcatcatttg catagtatcc tttcccagac
     2701 aacatttttt ctttttcagt catatagttt actccttttt gttataattg tatctaaagc
     2761 aaaataaata ttcctgtgga aaatctaaat atattctgtc acctttctta aatctcattt
     2821 tacttttttc caattctatt tgaataggta cacattcgtt agataaatca gtttttctaa
     2881 catatatagt atatgtaaaa ggattttcta caatgttaat caattcgaaa cataactttt
     2941 catttaaatc ttcattagaa tccaagacct taatatgatg ctccctgatt cccacatatt
     3001 ctatattatc acctatttct ctatttaatg tcaactcgca tccccagtct gtagcataaa
     3061 tagtattttt atccagtcta tttaatttgg atatattttt acaccctgtt atcttagctt
     3121 ctataaggta ctttggagat tcaaagagct cttttttagg tctctttgga agaccttctc
     3181 ctttgttgta cactataata tcatcacaaa ctcggtaagc ttcttcaata tcatgagtaa
     3241 caaatagtat atctcccttg taatctttta acatattcat aagttctttt tccatattgc
     3301 ttcttaaatg atggtccaat gctgagaaag gttcatctaa aagtaatata tctggagatg
     3361 ttgccaatgc tctagctagt gctacccttt gttgttgacc accagataat tgccaagggt
     3421 atctcccctc aaacccattt aatttaagta tatctaagta ccttgctact atttcctttt
     3481 tttcactctt acttagtttt aaaagcccaa gttctatatt ttgacttaca gtcatatgtg
     3541 gaaacaaagc atagttttga aataaaaatc ctacctttct atcttgtgtt gataaattta
     3601 tcttcttttc agaatcaaat agaactctat catttaatac aattttacct cttgttggtt
     3661 tctcaagccc tgctatacat tttaatgtca tactttttcc agaaccagat tctcccaaaa
     3721 accccagagt acctttttct tgtttaattt ctacctttag tttaaaagag gataaatctt
     3781 tttcaatatc cacgtataag ctcaactatc cacacctctt tcctataatt ttctgctgaa
     3841 attccgacca ataatttagt aataaaatca ttgctataga tattgctaca ataatcataa
     3901 cccacatcat agctttattc atatctccgc cttcaacagc aaaaaatata gctattggca
     3961 tagtttgagt ttttccaggt atatttccag ctatcataag tgtagcacca aattctccaa
     4021 gagctctggc aaacgacagc accaatccac caattattcc aggccatgct aatggaactg
     4081 caatcttaaa aaatactttc cattcattaa gacctaatgt tctagcagct gacaacatat
     4141 tattatcaat ctgttcaaaa gctgaccttg aagttctata catcatagga aaagacacca
     4201 ctactgctga aattacagtt gctgtccaac taaatattaa agtcctatcg aaatttaata
     4261 aaaactttcc tataaatcca ttcttcccac atagtaataa taaaaagaaa cctacaactg
     4321 ttggtggcaa aattagtgga agtgtaaata gtccatctat aagacccttc cacttacctc
     4381 tatagttagc cataatatat gacacaaata ttcctattac aaaagttatt attgttgaca
     4441 aaatagatgt cttaagtgat atccataagg gtgaccaatc tgttcccatc tttccctcct
     4501 taattttaaa tggaaatagt gaaactaata actctattaa tttcactatt tccattttat
     4561 tataatacac aattattttc aattttcatt tatttatata taatttatac ttaattacac
     4621 ataatttatt tctaagttta ttctactttt ttatatccaa attcttcaaa aatcttttgt
     4681 cctgactcac ttaataaaaa ttcttcaaat ttcttagcag catctacgtt tttactagct
     4741 ttaattactg aaactggata tgtaataggc gaatgagttt tttcgtctgt tttttcaact
     4801 acttttatct tatcattatt gacagtatca ctgaagtaaa cgaatccaac atcagcattt
     4861 cctgattgca cccatgctaa aacttctttt acatcttttg caaatactag cttatctttt
     4921 aacttatctt ttaaatttaa attagttaac acctcatctg catacttacc agctggaaca
     4981 ctttcagctt caccaacagc aattttcttc actttatctg tagtcaaatc ttccatacct
     5041 gatacactac tgtcagcaga agaaataaga actaaatcat tttttactaa atctttcata
     5101 gtatcagaaa ctaataactt ctcttcatcc aaagctttca tttgtttttg acctgctgaa
     5161 ataaataaat cacaaggtgc tccttgttct atttgttgtt gtaatgagcc tgatgctcca
     5221 tagttaactg taagttttac atttgaatca acttttttat attcttcttc aatctttgcc
     5281 attgcttctt ttaaacttgc agcagccgat atatttaact ctactgaatc actactttta
     5341 gttgtttttt cttgttttcc atcatctttt ttgtcttcat tactattgca cccaactgtc
     5401 cccaaagtta aacatgcaac taatcctaat attcccaata tttttttcat aacaatctcc
     5461 ctttttgtaa atttgataca cattttaaca tgttttatta tgttttatca tgttttgtta
     5521 tatatagttt aattatatat acatgataat aaattttcaa gtaatttatt tacactactt
     5581 ttaaatattt tgctacattt tccttttttt aatgttaaaa taaccttata tggaggtgga
     5641 ttatggactc attatcatct ttgacacctg tcgaggtagc tgaattatta aagataacaa
     5701 aaaatactgt atatgaatta atcaaaagag gtgaacttcc ttcttataaa gtagggaaaa
     5761 aaataagagt tgatactaaa gatgttgaag aatatatcaa cagccaaaaa actggtaaag
     5821 taaaggatac acatttcaac aataaaaata tagatataaa taataatttc aaaattatta
     5881 tttctggtca agatgtaata ttagacattt tagctaggtc tgttgaaaaa aaattggatg
     5941 gaataagtac atttcgctct tatataggta gttataatgc tttgtatgag ttatataatg
     6001 gcagagtttc tatagcatca tcccatctat gggatttcga aacggatgaa tataatagca
     6061 cttttgttaa aaagctattg ccaggaatac catgtgtact tataaatcta gcttatagaa
     6121 tgcaaggttt ctatgttgct aaaggaaatc ctaaagagat aacaacctgg gaggatttaa
     6181 caaaaccaag tataaccatt gtaaataggg aaaaaggttc tggaactagg gttttactag
     6241 atggtaaact tagacttcta aactttaatg gtaaacatat aaaaggttat gataatgaag
     6301 aattatcaca tcttggagtt gcaagtactg tttctcgtgg aatagcagat gttggtttgg
     6361 gaaatgaaaa agctgcttta caagtaaata atatcgactt tattcctctt cacaaagagc
     6421 gctatgattt agttattaaa aaagaagact tacaaaatcc agtttaccaa actataatta
     6481 acataataaa ctctcctgag tttaaagctg aacttcaagg tcttggcgga tatgatttaa
     6541 ctgatactgg aaaaactata gcaaaagttt aagattaaaa tcgcaatcta ttttataaga
     6601 tttataattt ttcacaaaat ttataactta aacacgaaat aaattgcgat tttagtgtct
     6661 caaaaaattt atgtctttca ttttatcatc caatacaaaa tataatataa ttaattgtgc
     6721 aatcatatta tattaatata tatttacata atagtatttt tctttaatgt agattaacat
     6781 atgatatgtt ttaaacatca atccccttgc tttcttatat atatttggca acacatatga
     6841 gtttaataaa ttatatacaa tgttgctttc ttcttgtatt ttttcaacca acaagttatt
     6901 tataaaatta cctttctcat tttcaataat ctcactccaa tttatcttaa cttttaatat
     6961 atatcttccc actaggtata cacactctac aataaataat aaaaatacaa tgcagtcagt
     7021 acctgttgtc ttatataaat tacccgatac taatggcttt gcgaagaaat tataaaaatt
     7081 caaagtatca cccgtataat caaaaagtat taatagcact cctagaactc tcaatacttt
     7141 aatattcaac atgtttttat ctttattatt tttgtttttc atatatacta cctctctttt
     7201 ctttagattt gattgtaatc taatactaac tcttttattt gaggtataaa tgaatttaat
     7261 tggtagttat tttcattttt actagaaatt aattcacatt actaaacaaa acttatattt
     7321 aaacataaaa atagcctatc tatcttttaa taaatacaaa aataaataag ctactaatat
     7381 tcaattaact tttatcactt aaaacattac tatctataaa tttaaatatt ttattgaagt
     7441 atactgtact atcatgttca aatccatcaa catgaccaat accttctata agccataggt
     7501 cttttttatc atgtttaact aaatcataca ttttcttgct attttgataa ttacaaacag
     7561 tatcttttgt tccatgtata aacagtatgg gtacattaga tttctcaact gactcatatg
     7621 gttttacatc actgtaccaa aatccagatt taaacttatt atataaatct ccacaagtca
     7681 ccacataatt gattaatata tctggtattc gtttttctag tactcccatt ctaattgcat
     7741 ctttcatttc agaataagga gaatccagta tataaaaatc gaccttatca tcttttgaat
     7801 ttatctctga atgcatagca gctgtccctg cccccatgga aaatccatgc acacctaatc
     7861 tcccttcagg aaatttattt tttacaaatt taactagact atctaaatca tacctttcat
     7921 aaagaccaaa tgtgtaatta tctccaccac tattaccagt atttctttga ttataaacta
     7981 atacattata acctttatct aaatatctat atgctacctt taacatttca tagtaactac
     8041 ttccaattcc atgaacaagt atcatagtat ctcttgtctt tatatttgaa gttataaaaa
     8101 gactttcaag cttgtaatta ttagaacttt gtatcattaa ttcagatgtc ttgtagtttt
     8161 ttaatttctc cagtggttta tcttttctct cagaaaatat ttttactata tcttcgtttt
     8221 ttattaactg ctctaaacca actgaactat tatacactaa ttttccaaat atataagata
     8281 caattaatag gattaataga aacacaattg atataattat aaatttattt cttaaactaa
     8341 tacaattttt aaaactattt ttcatattta tttaccatac aattttccta tattatat
//
LOCUS       DAMJWU010000129         8370 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMJWU010000129 DAMJWU010000000
VERSION     DAMJWU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.134"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(59..529)
                     /locus_tag="PC460_003272"
     CDS             complement(59..529)
                     /locus_tag="PC460_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2602129.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(1263..1652)
                     /locus_tag="PC460_003273"
     CDS             complement(1263..1652)
                     /locus_tag="PC460_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2602130.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(3451..4857)
                     /locus_tag="PC460_003274"
     CDS             complement(3451..4857)
                     /locus_tag="PC460_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2602131.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVEFDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(4902..5609)
                     /locus_tag="PC460_003275"
     CDS             complement(4902..5609)
                     /locus_tag="PC460_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2602132.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            5780..6694
                     /locus_tag="PC460_003276"
     CDS             5780..6694
                     /locus_tag="PC460_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602133.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QKLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            6694..7455
                     /locus_tag="PC460_003277"
     CDS             6694..7455
                     /locus_tag="PC460_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602134.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMFMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            7467..8204
                     /locus_tag="PC460_003278"
     CDS             7467..8204
                     /locus_tag="PC460_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602135.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         2808 a         1364 c          872 g         3326 t
ORIGIN      
        1 ttatcttgaa ttagttctat tacaattaat ttctactaaa taattataat ttaaacaatc
       61 attccttact attttcacac tgctttacaa atttaattgg atgttgaatt attttagaag
      121 tttcttctac agagtatccc tcagataaat atcttctagc tataacttcc atagactctc
      181 ctatctcaat aatatgctta tccgggtcat aaatacgcac tactctttgt tgccattcat
      241 gttttagtgg cttatggaca aactctacat ctttataata attgagtttt tcaataaatt
      301 cgtcaaaatc atcaacctca aaataaagtt ccatattatt tgatttttgt ataattgtat
      361 ctggattaat ccctgtaagc catgcgaagt cttcttgtat agcaaatcct cctttaaatg
      421 ttacattttt acctaagtcc aattcaattt cttgctcaaa taattctttg taaaattttt
      481 ttgatacttc aatatccttc actgctaata atgaaacctg atatttcata aattttcctc
      541 ctaatttttt atttctattt tttcagtatc ttctattaac atttttttct ctatctcttc
      601 agtacctttt attaatcctt tatttttact tttttagtat cttctattaa cacttttgtt
      661 tctacctctt cagtatctta tatcaatctt ttattttttc aatatcttct attaacactt
      721 ttatctctat ctattcagta ctttctatca atcttttgtt tttatttttt caatatcttc
      781 tattaatact tttgtttcta cctcttcagt atcttatatc aatcttttat tttttcaata
      841 tcttctgtta acacttttat ctctatctct ttaatatctt atattaatcc tttattttta
      901 ttttttcaat atcatcatta attcctattt attttatcat cttaaatacg aaaacactgt
      961 gtcatatttg caaaatatat attatcatct atattaaagc ttcacaattt atatttaaaa
     1021 attctcttta attacactac ctctatttaa aaagctacct taattatact tataagtaaa
     1081 ggtagctttt taaatttaat taaatagaat atccactatt aatttttatt taatttattt
     1141 agattcacat tatagtatca taatccactt ataatcattt gtcatattac ttaagtagac
     1201 taatatatta cataaataca cttacataca ctttatattg atgctataag aaatacgttt
     1261 gcttatacgt ctttctcctc ttcttttaga ttttttatcc actcttcaat aagtaagata
     1321 tcatctttgc tcaaattaat attttcatgt aatgacaaca ataaactttt tatagaagaa
     1381 tcatgaatat ttctaaaaaa atctcttgtc tcaactccta tgtattcctt ttctttaatt
     1441 aaaacttcat aatgcgtata tttatctatc ttttgagaat ttagaaatct ttttactact
     1501 aatctagata aaagagtcaa agttgtcgtt tgtttccatt gatatttttg ctccattcct
     1561 aaaacaatat ctctagatgt tactttggaa tctgatttcc atataaattt cattatcttt
     1621 aattctgatt gtggcaactt ttttatagtc atgataaaac cttgctactc agaatataga
     1681 caagcaatat ccataataaa atttttcgat taaatacatc ttatacggat ttatataagc
     1741 tcagacttat aagatatttt tcaatcttaa tcctaaattt agctatttgt acacggtaaa
     1801 tatatataaa taatgtcaaa attctcttgt attatattgc ttaattttat gaaatatttt
     1861 gtactcagct atctattttg gatacttgtc cagtagctct cctttctttt aaactctttg
     1921 attttaaata tttgtatttc agtaaatcct acttcctaaa ttttttattt actacaactt
     1981 attactgaac ttcctacttc ctaaactttt tatttactac aacttattac tgaacttctc
     2041 acttactaaa tttcttactt actataaatt ataactaaac ttctcactta ctaaatttct
     2101 tacttactat aaattactac taaacttcct actttctaaa tttcttactt gctataaatt
     2161 attactaaac ttcctacttt ctaaatttct tacttgctat aaattattac taaacttcct
     2221 actccctaaa tttcttactt gatacaaatt attactaaac ttcctactcc ctaaatttct
     2281 tacttgctat aaattattac taaacttcct actccctaaa tttcttactt gctataaatt
     2341 attactaaac ttcctacttt ctaaatttct tacttgctac aaattattac taaacttcct
     2401 acttcctaaa tttcttactt gctataaatt attactacta aacttcccac ttcctaagtt
     2461 tcttacttgc tacaaattat tactaaactt cctacttcct aaatttctta cttactacta
     2521 cttataacta aacttcccac ttcctaaatt tcttacttac tactacttat aactaaactt
     2581 cccacttcct aaatttctta cttactacca cttataacta aacttcccac ttcctaaatt
     2641 tcttgcttac taccacttat aactaaactt tttacttctt aaatttcttg cttactacca
     2701 cttataacta aactttttac ttcttaaatt tcttacttct taagaataat gttaatttta
     2761 agattatagt ttttgccttg tttaattgaa acattctata taattatatc cttctatttt
     2821 agagtttttt tatttatata ttacaaagtt taaatttttt cattaattat attataaatt
     2881 tcccatatat ttcaacataa atacatatat atatatgtta tttctaaaaa tactacacaa
     2941 aaactctact ttagtagaac tcaatggcgc aaaatacttc aatttaaaac atttatcgta
     3001 ctttaaataa ataactataa taatatactt acaatagtta catagaagct attataacta
     3061 cacagaacag cgtataatta aaactttttt aggatgtcat tatatttttt cttaaaatta
     3121 aaaaacacta taccataatt ctattttagt agaatttttt tgtcaataat aaaattaaat
     3181 attaactaaa aaataaaaaa aatggaatta tctaaagaat ttaataacct tacaataatt
     3241 ccaaaatatt atttctatga aaattttgat accatgagta actcagccat aagcgcataa
     3301 agcacaagtt actctttact ttaataaaac ttgacaagaa aagaatttat ccttattcta
     3361 ctctaataga gttaatttgt caataaagaa taaaattatt ttcaaaatat tttcattctt
     3421 cattcttatc tgttattatt ttataaaata tcacttaatt agaggtaatt ttagtattgt
     3481 ttttagtccc tgactatcaa tgctcgtaat atctaattta cctctatgtg cattaataat
     3541 gccagatact atcaaaatac ctagaccatg attttgacct ttggcatatg cctcacttag
     3601 tccattttgg ctctttaata aactattaat tctttcttga ctcatacctt ttccatcatc
     3661 ttcaacaatt atatagcaaa aatctaaatc agacttagag gtaaccctta tattacatcc
     3721 ttgttggtta tgagttatac tattttgtat taaattacta atagcccttt ttaataattt
     3781 tttatctgca tctataataa tatcttcatt tagtatatca aattcaacgt aaaagtcatt
     3841 atccaaatta ttatttataa actcagaaat aagctctctc aaaaacgcag aaactctaat
     3901 cttatcacaa ttcaatgctt gcatattata ttcaagttta gacactaaat ttaggtcatt
     3961 tactaattct ctgattttaa tcccttgatt gcatataata gacacttgct cttgttctct
     4021 atttgaaagg ctactgcttt cttcaagttc tccagcatat ccaattatca ttgaaagtgg
     4081 agttcttata tcatgagata ttcctgcaat ccagttagac ctagcttcct ctttcttcct
     4141 aagtttttca tctttcattt gcaactcttt agatacagta ttaatagatt ttgaaatatc
     4201 acttagtatt cctttttctt gtaaaagtac tggttctcct tttgacataa gctttatgcc
     4261 agtaataatt ggttttatag atttcaccat ttttaaacct atgaatagag ctattaaaaa
     4321 agtaactatt gcattaacca aaaacatacc tattattaac ataggaaatt gttctatttc
     4381 cttaaccgta taagtaaggt taaacttagt gtacttgtct tttggataac ctgttactaa
     4441 tattccattt ggatgttccc atacatacac aggatagtcc tttaaaaaga accttgaaaa
     4501 ctttgctaca tctgtcaaag tataatgact tggaatttct tttggtaaat caaatcccca
     4561 tattctatct ccattgttat caattaacat tgcccaaact ttatttttat gtagtacttc
     4621 cttactagaa tcatctaaaa taaactttcc atttcttaaa atcaaattat cttcaatcat
     4681 aatcaagttt gattttattt ctctgctatc acttgtatct aaattatcag agtatttaga
     4741 tacaaatagc atatatgaaa acagaagaaa acctgctata tttaatacaa taaaaagtaa
     4801 tgaaatcaag atacttgaac ctataaatcg cctcaatatg tactttacac tatccaaaat
     4861 taaatcactc ctcttgattt cttttattct aatatctaaa attaattaat catcaactta
     4921 tatccaagac ctcttactgt aataagatgt ttaggtgtag atggagtttt ttctatcttc
     4981 tctctaaccc ttctaacatg aaccataagg gtattttcat atccatacat atcatctccc
     5041 caaatggctt gacatagtga atcattagta acaattctcc ctttattttc atacaacttt
     5101 aaaagcaacg aatgctcttt agctgttaaa gaaatctctt gcccatcttt ctcaacacat
     5161 gcactattta aattcacaac aacagaatct cccagtttaa atactggcaa ctcctcttct
     5221 tctataggca catatacacg atttaatata cccacaagcc ttaaagtaag ttcctttggt
     5281 aaaaatggct ttactatata atcgtcagcc cctaagccca aaccaatcaa tctatcttca
     5341 tcttcacctc ttgcagagag aaaaatcact ggtattttag atgtctctct aatttttctc
     5401 ataatagaaa aaccatctcc atctggcaat acaacatcta aaattgcaca atctggcttt
     5461 acatttctaa ataccctaac agcttcctca taacttgatg ctaaaaatac tctactaaac
     5521 ccctcttttc tcaggatttc atctatcatt tttaataatt ccttgtggtc atcaaccaca
     5581 agtatttttt tatcttttat tgactccata atcatcaccc tcctttagta taccatatac
     5641 ccattttcac aactttgcct aaatagactt ttaaggttat tgtaaggtaa atttcagcta
     5701 attgaaatgt aaccaagtta ttatactgat ataagttaaa agaatcaata agcacagatt
     5761 aaaaaagggg atgaaacata tggaaatgat aattagaaca caaagacttt caaaggaata
     5821 caataaaata tttagagtaa aggatataga cctaagagtt ccacaaggag ctgtatatgg
     5881 ttttttgggt cctaatggag ctggaaaatc taccactctt aaaatgcttc ttggacttgc
     5941 taaacccaca caaggaaata ttaatatttt aggtaaagag ttaaatgaaa aaaatagaat
     6001 atcaatttta aaagatatag gttctttgat agaatctcca tcatactatg gtcatcttac
     6061 agggcttgaa aacatgacca taatgcaaaa actcttagat ttacctaaaa aaaatgtaaa
     6121 tgaagcatta aaaattgtaa ggttagaaaa tcaaaaaaat aaaaaagtta gtcaatattc
     6181 tttaggtatg aagcaacgtc ttggtattgc tatggcaata atgaagtttc ccaaattatt
     6241 aatacttgat gaacctacaa atggactcga cccagcaggg attgaggaaa ttagagaatt
     6301 aataaaatct ctaccagaaa aatacgggat gactgttcta atctctagcc atcttttaag
     6361 tgaaattgac caaattgcca catcaatagg aataataaat catggcgaac tggtttttca
     6421 agatagcata aaagaacttc ataatagagg tcaaagtcag attgctatca aaactaagaa
     6481 tgttgtatct gcacaaaaac tattaaatac taaaggatat atgtcactta tacaagatgg
     6541 gtacttgact attgaaaatt taaaagactc agatgtggct aaaataaatg gtatgcttgt
     6601 ccattcaaat atagatgtat ttagaataga agaacataaa aaaagcttgg aagatatatt
     6661 cttagaactt actggtaagg cggtgacttt ataatgaaat accttggtct agaattttat
     6721 aaacttaaaa gaaagaagat tattctgatg acctttttat ttgtagctgt tgaaataatg
     6781 tggtgtatca taaattcaaa tagagctatt acacgtaatc cagatatgtt aggtggattt
     6841 gaatattcct atatgtttat gagttttgca agtttaaatg gtctattctt tcctatctta
     6901 atatcaataa ttacatcacg tataagcgat atagaacata aaggagatac atggaagctt
     6961 ttaaaatctt ctgtgacatc tttaaatagt atttatttat ctaaattttt atgttctgct
     7021 attttggtat ctatacctgt attgatgcaa gtgttatcta ttgtgttgtt tggacatttt
     7081 agaggggtta ttgagtcaat ttctgtatct ttacttttaa aatacacatt aggaacaatt
     7141 cttgtaagta ttgctatcat tgcactacac ctatggattg caactgtaat tccaaatcaa
     7201 atgattgcaa ttacatttgg catgattggt agttttatag gtttgacatc aggtttgttt
     7261 tatcaaggta ttcgtagatt gtttatatgg tcttattacc tagagttatc acctttaagt
     7321 tatgcttatg ataatgtttt aggttcatct gtctatagaa ttgatatgaa tttttctatt
     7381 acgattttag ttttcttaat tggaatgcta atttattata ttggaagaaa tcatttatta
     7441 aaaaaagaag tataggaggg atttaaatgt taaggaagtc agttttggga gaaattctaa
     7501 agttaaaaaa ttcttttata tggtatgtat tattagcttt gccacttata agtgtgttga
     7561 ttggttctgg taatttttat ctaaatcaag gcatactcaa aaaagaatgg tacagcttat
     7621 ggactcaagt tagtcttttt tatggggaat ttttctttcc tatcttaatc gcaatctttt
     7681 gtgcatatat ttgtaggctt gaacatatga atcataattg gaataatgta atgacactgc
     7741 cagttaattt aaaaaatatc tttttatcta aacttacagt agtttcaata cttacagcta
     7801 ttacacaagt tttctttgtt atcttttata tagttgcagg aaatatgttt ggtttttctg
     7861 gtagtgtacc taaagaattg actggttgga ttgctaaagg ttggattatt tctatttcaa
     7921 tagcttctat acaattatac atgtctataa agattagaag ttttgcaact cctattggta
     7981 ttagtctttg cctttctcta tttggtatgg gattaatagt agcaaaacct gctgttggta
     8041 tatttttccc ttattctatg ttaggtagtg ctatgggggt tataaagcaa tctgctttaa
     8101 atgttaatga cactatagtt acaatatgtg ttgcatttat tactactata gtattttctg
     8161 aaattgccaa tagaaatttt agaaagaatg atgttatttc ttaatggtaa atgctagata
     8221 ttttggggtt tgaaatttat attcttttta gttgaaatct atatccattt tatttaaaaa
     8281 tatatatctt ttatttgaat tttatatgtt ttatctactc gctatataat agatatactc
     8341 ctaaaattaa aaaatatata ataatgttat
//
LOCUS       DAMJWU010000130         8032 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMJWU010000130 DAMJWU010000000
VERSION     DAMJWU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.135"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     misc_binding    91..301
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            527..1342
                     /locus_tag="PC460_003279"
     CDS             527..1342
                     /locus_tag="PC460_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2602136.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            1370..2047
                     /locus_tag="PC460_003280"
     CDS             1370..2047
                     /locus_tag="PC460_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2602137.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            2106..2828
                     /locus_tag="PC460_003281"
     CDS             2106..2828
                     /locus_tag="PC460_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2602138.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            3476..4627
                     /locus_tag="PC460_003282"
     CDS             3476..4627
                     /locus_tag="PC460_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF2602139.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRLSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            4673..5848
                     /gene="thiI"
                     /locus_tag="PC460_003283"
     CDS             4673..5848
                     /gene="thiI"
                     /locus_tag="PC460_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF2602140.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            6004..6807
                     /locus_tag="PC460_003284"
     CDS             6004..6807
                     /locus_tag="PC460_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2602141.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFSQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            7050..7691
                     /locus_tag="PC460_003285"
     CDS             7050..7691
                     /locus_tag="PC460_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2602142.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         3203 a          780 c         1527 g         2522 t
ORIGIN      
        1 ataatttaca agtaaaaatt attaaaaaaa taaaaaactt attgacaaaa atattataag
       61 gaagtacaat tgtattaaca atttaaggaa acaaatccaa tgatagagaa agtagcatat
      121 atggatttac agcgagttag gggttggtgt aagcctaaca atgaagtcta tgtgaagaga
      181 gctctggagg agattatcag aaactttagt atgataattg gtttacccgc cttaagggat
      241 tgagaggata agtacttact tatcaaaaag agtggtaacg cggatataat tcgtctctta
      301 gctgtaaagc taagggactt ttttgatttt aaaataaaaa tatccataca gcttaaaagc
      361 taacagtcac aaattttgaa agtaatataa gtatttatca ataagagtgg taacgcggta
      421 aagtcgtctc ttagtcatta aagactgaga gacttttttt gtaactcgaa acatttaagt
      481 attaaaatct gagggaaatt ataattaaaa gagaggggta gttaaaatga aagtatttaa
      541 aaaattatta agtttaggat tagtattagg attgacatta tcgctagtag gatgtagtgg
      601 tggaggagaa aaaacaaagt tagaacaaat aaaagacaat ggaaaattag ttgttggtac
      661 tagtgcagaa tttcctccat ttgaatttca taaggtagta gatggaaaag attcaataaa
      721 aggattcgat ataatgttgg ctgaagaatt tgcaaaagag cttggtgtaa aggttgaaat
      781 taaggatatg tcatttgatg gattaatagg tgctttaaat gcagaccaag ttgatattgt
      841 tcttgcaggt atgtctccaa caccagagag agaaaagagt gttgattttt cagaattata
      901 ttatttaagt agaaatgcag ttatagttaa agatgcagat atagacaaag taaaaactga
      961 agatgattta aagaaactta gagttggagt tcaagcagga agtatccaag aagaatatgt
     1021 agttaacact ttaaaaatga caactacaaa atctttgaaa gcaatacctg atttaattac
     1081 agaattaaaa aatggaaaca tagatgcagt tgttactaat gaagcagttt ctttaataaa
     1141 tgttaaaaaa tatgatggaa taaaaatggc aaatactgaa gttgggaaag atgtaacaga
     1201 aggtatggca gcagcaatca aaaaatctga caataacaaa gattttatag aattattaaa
     1261 caaaaagata aaagaattac aagatggcaa gaagatagaa gaatttttaa atgaagcatc
     1321 tactgaagct gcttcaaatt agtaagaaag ttatgtggga gatgaaatta tgagtttaga
     1381 ttttagtttt ttaagtagat ttgggacttc ttttttggaa ggaacaggtg tgacagtatc
     1441 aatttctctt gtggcattat gctttggatt tataataggt ataattatct gtatggcaaa
     1501 aatatcaaag agcaaagtat taagagcaat atcatcaatc tatattgagg ttttaagagg
     1561 gacaccatta ttagtacaga tatatatagt atggtttggt ttacctcaat taggaataag
     1621 attccctatg ttgtttggta taccaagtga atttatagct agtgcatttg cattatcagt
     1681 aaactctggt gcatacgtgg cagagatact tagatcaggt atacaatcag ttgataatgg
     1741 acaaatggaa gcaagtagat cattaggatt aaattattgg tcaactatga gatatataat
     1801 aatacctcaa gctataaaaa atatattacc ttcattagca aatgagttta ttacacttgt
     1861 aaaagaatct tcaataatat ctgtaatagg agttgttgag ataatgcgta ctgctgatat
     1921 agtaaaaaat gcagcattta gagcattaga gccattaata gtagcagcag caatttactt
     1981 tgttataaca tttactctat caagattagt tggattatta gaaaagaaac tatctgtgtc
     2041 taactagatg agagcatgtt tagcatttag ggaaaacata gaatagtaaa aaggaaagtg
     2101 ataatatgat tacaataaaa aatttaagta aatcttttgg agatttaaat gtattaaaaa
     2161 atatagactt agaaatcgct aaaggcgaaa taatggttat agtaggtcca agtggttctg
     2221 gtaaaagtac acttcttaga tgtatgaatt tactagaaat acctacaggt ggagaaataa
     2281 tatttgaagg aaaaaatttg gtcgataaaa aaactaatat tgatgaggtt agacaaaata
     2341 taggtatggt gtttcagaac tttaatttat tcccacataa gactatttta gataatataa
     2401 cattagcacc aattaaatta aaaaagatga ctaaggaaga agcagaaaaa aaagcagaaa
     2461 ttttgttgag tagagttggt cttttagata aaaaagattc atatccatca caattatctg
     2521 gaggacaaaa gcaaagaata gcaatagcta gagcacttgc tatggaacca gatatgatgt
     2581 tgtttgatga gccaacatct gctcttgacc cagaaatggt aaacgaagtt ttggatgtta
     2641 taaaagaact tgctaaagaa ggtatgacaa tggcaattgt aactcatgag atgggatttg
     2701 ctaaggaagt tgcagataga gttattttta tagatggagg aagtatactt gaagataata
     2761 ctccagaaga agttttcgga aatccaaaac atgaaagaac aaaagcgttt ttagcaaaag
     2821 tgctataata tataaaaagg atgttattga tattagaaat tcaatacatc cttttttagt
     2881 gatggttaat attaatttta aaattaaagt tatattaata ttaactttat ttttacgatt
     2941 ttttgcagaa aaaaaaagag ctcggggtaa gctctttttt tccaccgaat taatttccgt
     3001 tactaatccg atttttaata aatataaaat atatccttat gaaagggtat tgggaataga
     3061 tatattaact aactagggtt accaggggga taaccttaat ttattataac aaaatgcgac
     3121 acaaaaatca atattttttt tgaaataaat tataaaagtt atttttataa caagtaagct
     3181 tataaattta acttttatag taaaaataag tatatgtata atataatata tttattttat
     3241 ataatatatt attttatcat atattgtatt ttataatata tttttgtagg gtatagtata
     3301 agtatattat attttactat aaaaatttgt aataaagtga aaaaactagc ttttcatgaa
     3361 ataatatttt ataatttaag attgtctttt taataaagat taacaaaaaa aatactataa
     3421 ttagtggtat aatagttaga gttgaagatt ttaaataaag gtaggtgtaa ttaagttgga
     3481 aatatactta gacaatagcg caactacaaa accttatcaa gaagttatag ataaaatggt
     3541 ttatgcactt aatacggaat atggaaatcc ttcttctgtc catagaaaag gtgtagaagt
     3601 tgaaaaagct ataaaagaag taagacaaga tatagctaaa tctctaggag cgaaagaaaa
     3661 ggagatttat tttacatcag gtggtacaga gtgtaataat actataataa gaggtattac
     3721 tagtttaaat aaaaaaagaa aaaatcacat aatatctact aacatagaac atccttctgt
     3781 tttaaataca ttaaaagatt tagaagaaga tggttttgaa gtaacttatt tagaggtcgg
     3841 aaaagatggg aaaataaata tagaggattt aaaaaatgcg ataaagtcaa ctacatgctt
     3901 agtaagcatg atgcatgtta acaatgaaat aggaactata cagccaatag gtgaagttgg
     3961 aaagtatttg aagggtttaa aagaaaaaat ttatttccat gttgatgcta ttcagtcata
     4021 tggaaaaatt aattttagac tatctaaata caatatagat tttatgagtg taagtgcaca
     4081 taaatttcat ggtccaaagg gaattggatt tatgtatata aaagaaaata atcgccttaa
     4141 gcctatgtta acaggtggtg gtcaggaaat tggaattagg tcgggaacag aaaatgtacc
     4201 tggaatatat ggtcttggag aagctgttag aatactaaac aaagatttag atgttgttat
     4261 ttcgaaagta aataacttaa aaaatatatt aaaaaatgaa ataattgata atatagaaga
     4321 tataaaaatt aattctccag aagatggagt atgtcatata ttaaatgtat catttagagg
     4381 gacaaaagga gaagttttac ttcattactt agagcaaaag ggtatttatg tatcaactgg
     4441 ctctgcatgt tcttctaaaa agaaaggtag ttacgtatta aatgctatag gtcttacaaa
     4501 tgaagaaata aatggaacaa taagatttag tttatctgat atgaatacag aagaagaaat
     4561 gttagaagct gtgaaagttt tgaaagaatc aatttgtgac ttaaggtcaa taatgaaaag
     4621 aaaataaaga atactgtata gatagtgata ataaattagt ttaagaggtg aaatggtgta
     4681 taatatatta atagtaaagt atggagaaat tggagtaaaa ggaaagaata gatatatatt
     4741 tgagaatagg cttataagaa acataagaaa tatgttaaaa ccaattggaa gatttaatgt
     4801 atataaagag tatggaagaa tatatgttga tttagaagac tatgattatg aagaagtaat
     4861 tgaagaagtt agaaaagtat ttgggatagt gggtgtgtgc cctggagtaa gagctaaaaa
     4921 agattatgat acattaaaag aaatagcttt aaaaatgtta gaagaaaaaa tagaggctgg
     4981 atataaaact tttaaggtag agtcaagaag aggagataag tctttcgggt tgacttctca
     5041 agaaatgagt atggatatag gtggatactt attatcaaag gtaggagata ggattaatgt
     5101 tgatgtaaga aatccagaag ttaaaataaa gtgcgaatac agagaatttc atactatggt
     5161 ctatagtgat actatacctg gatatggtgg attgccactt ggaacgaatg gtagagctat
     5221 gtctctttta tcaggaggta tagatagccc agtagctaca tggatggttg caaaaagagg
     5281 tatggaagta gaggcagttc actttcatag ttatccattt acaagtgaaa ggtcacagga
     5341 aaaagttaag gatttagcta aaatactggc aaaatactgt ggtagagtaa ggcttcataa
     5401 agttaatatc ttagaaatac aaaaagcaat tggcgagaat tgtaatgaag aagaagctac
     5461 tattctttca agaagattta tgatgaggat agcacagaga ctttctgaaa aaagacattg
     5521 tgatgcttta attacaggtg aaagtattgg gcaggttgca tctcaaacta tacaaggact
     5581 tacttgtact aatgccgttg tagacttacc agtgtttaga ccacttatag caatggataa
     5641 atcagatata gttgatatag ccaaaaaaat agggactttt gaaacatcta tattgcctga
     5701 agaagattgt tgtagtgtat tttctcctag aaagcccgtc actaaaccta gattagagaa
     5761 aatagaaaaa tctgaaacag cacttgacat tgaaaaatta gtacaggatg ctattgacaa
     5821 aatagaggta gaggatattg agttttagca tacaagttat ttaattacta aattgaatat
     5881 tgagtatatt agtagttctg taagtatatt agtagttctg ttgaaaggat ttatgtagac
     5941 aatttattaa tatgtagtat ttaaaaataa gtactataat tttatttaat gggaatgatt
     6001 attatgaata aaaagggttt tacactaatt gaattgttag tagttatatc tataataggc
     6061 attttagtta tagtagctgt tccagcattg tttagaaata tagaaaaaag taaagcagtt
     6121 acatgtcttt ctaatagaga aaatataaag actcaaattg ttattgcaat ggctgaggaa
     6181 tcaagtaaag gcaagaatga agtcatgaaa gaggtattag aaaacaaaga tggtaagtac
     6241 tttgaaacag aaccaaagtg taagtcaggt ggaatatatt cagcaacgtt tgatgatggt
     6301 tatgatggaa taactggaat agaaagcatt gcaaaagtgt atgttacttg tacaaaacat
     6361 ccagatggtg ttgaaatggc tagggatata catcaaagta tgaaagattt gattgcatca
     6421 ttttcacaag acccttctat aataccagga gcctcaaagg gtaatgatga ttttagaaaa
     6481 tatttattag acaataaata taaaaatggg tggcctacaa ttccagatga atttaaggca
     6541 aaatatggat taagtaagga tacactatat atacaaccat atgcatatag tcctactaaa
     6601 tctgatgcta ctgtagttgt atttgcaaat aataagactg ggggtaattg gtatacttcc
     6661 ctagtttacg attatgatga aggtagatgg tataaaggta aaaatggtat ttctgttgca
     6721 ggtaggtcat gggatgttga cacagatagt gttaagtctg taaaaacaga gattcattct
     6781 aaagagggat ggggtccttt aaattaatat atcatgtttt attaatctgg tattgattaa
     6841 actatagaaa agagcagttt ctatagatga cgaataccag attttcattt atatatataa
     6901 ttgagatata ttaaaagtgt ttatattttt aagtcttcac atacaagcta aaaataatta
     6961 taataatatt gtaaagaaat ttaattataa gattaatttt ctgaagattt agtatttgta
     7021 ctaagaaata cataaaaagg ggaatagata tgaataataa ttttcaagac aaccaaacaa
     7081 taaatttaat acaaagtcgt agaagtatac gaaaatttac gacagaacaa attagtgatg
     7141 agcaagtaaa cacattatta cactgtgcat ttgcagcacc ttcaggatgt aataaacagc
     7201 cttggcacat aactgttgtg caagaccaga aacttttaaa agagattagt gatgatactt
     7261 tatctaggat tcatgaggtg agtaatgttg agataaataa aaactttaag ttattttatg
     7321 gtgctccaac cgttttattt atttcttatg atgaaagtag ttcatgggct ccatatgata
     7381 taggaatact tacagggaat ataacaacag ctgcacaagc actagggctt ggaagttgta
     7441 taataggaat ggttagaggt ttatttacac ctgttgaaca aggggatatt gaaggtcttg
     7501 tgagtgtatt ggataaagaa gatgtaaaag aatcagagtc aataaagatg aagtttgata
     7561 caaataaaaa atacagagaa ctacttgata taccagaagg gtattctgta ccatttggta
     7621 ttgctgtagg tattccagat ggaaatttac caaatgctag agaggtagtt tataaagttt
     7681 ccagagttta ggataaagta ttgcaagaac caatcagtta cttgaagaat taaaccagta
     7741 atatacaaat aaattgatgg aagaggaagc tatttaaaaa gtagtttaga tttttcgttg
     7801 taaaataatt aaataaagaa taaggtgata agtattttat ttattatctt attctttttt
     7861 atttttgttt tgtagaatga taaaaataag gattaagtgt aataaaatag aaaacaagta
     7921 tgttaatttt aagtatatgc tttaaaattt atccaagtta aaaatttaag atatcttttc
     7981 agtataatgg aaaaaatgtc aaaatattga aatagggaaa aaatagtgat at
//
LOCUS       DAMJWU010000131         7932 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMJWU010000131 DAMJWU010000000
VERSION     DAMJWU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.136"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(27..740)
                     /locus_tag="PC460_003286"
     CDS             complement(27..740)
                     /locus_tag="PC460_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF2602143.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1032..2696
                     /locus_tag="PC460_003287"
     CDS             1032..2696
                     /locus_tag="PC460_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF2602144.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2803..3702)
                     /locus_tag="PC460_003288"
     CDS             complement(2803..3702)
                     /locus_tag="PC460_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF2602145.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3729..4367)
                     /locus_tag="PC460_003289"
     CDS             complement(3729..4367)
                     /locus_tag="PC460_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2602146.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4704..5702)
                     /locus_tag="PC460_003290"
     CDS             complement(4704..5702)
                     /locus_tag="PC460_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF2602147.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KKISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5800..6375)
                     /locus_tag="PC460_003291"
     CDS             complement(5800..6375)
                     /locus_tag="PC460_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602148.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            6691..6954
                     /locus_tag="PC460_003292"
     CDS             6691..6954
                     /locus_tag="PC460_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602149.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNYENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(7391..7768)
                     /locus_tag="PC460_003293"
     CDS             complement(7391..7768)
                     /locus_tag="PC460_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2602150.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         2792 a         1216 c          836 g         3088 t
ORIGIN      
        1 aatataaata taagttttct atatttttaa ccaacagatg ttttcttcaa attaatcaga
       61 tgatttagta tatcttgaac ctttccagag tcctttaaat atcctaatgc cccatttatt
      121 tgtgatttta aatctatttc tacttccata tccaaatcaa catttccttt ttttatagat
      181 atatttttaa gtgttattgg ataaagataa cttttttcca aagtaatctt atttccatta
      241 acctcaaatg gaactttttt attgtagtta cttaatattt tttctagcat tttatcactt
      301 agtttaaatt ttccaatttt cacattctca agacttacaa ctaagtttcc atcctttaaa
      361 gtaggtctca attcaagttc atattgacta tttaccaaac caaatacctt atatggtcct
      421 gctaccttta ttttaccatt tttcatttca acaaaattat tttcaagttc attaattcca
      481 tgcttctgca tcaatgtata tattaaattt ttaaactctt cctctgaaac agatattttt
      541 ccaaccattc taagtggatt ttttactacc tccatagaat ttatatatga gccttttgat
      601 aaatattctt catctatgat tttattattt tgtgaactaa catcatatct ttcttttggt
      661 gtcaagatat atacaacaat tcctacaagt actaaaacaa taacaattaa acttaacaaa
      721 atctttgcaa cctttcccat aatcgtctcc tttacaaatc tgtcatcaga tttaaaataa
      781 atttagataa ctaacttcac ataatattct taacaatata catactataa tacagtcttt
      841 taaaaattaa ttcaaccaat ctaatgtttt gtaaataaac tgtaaaaaca tgtttttagt
      901 ataaaagtta atattttgca taatactctt aattacacct ttattttttt ctaatcttgg
      961 attaaaatta atattatgca atacagtgta gtgttctgta ttataatcaa attaaatagg
     1021 aggtatagaa tatgtcaaat gaaacgaact catctaactt tataaagaat attattataa
     1081 acgacttaga aacaggaaaa catgacagta tcataactcg ttttccacct gagccaaacg
     1141 gatatttaca tattggtcat gcaaaaagta tatgtcttaa ttttggatta gctaaggagt
     1201 ttaatggaaa agctaattta agatttgatg atacaaaccc attaaaagag gatgttgaat
     1261 atgtagagtc cataaaagaa gatgtaaagt ggttaggatt tgattggaac gagttaaatt
     1321 ttgcatctaa ctactttgat gaaatgtata agagagcttt aatactaata aaaaagggta
     1381 aagcttatgt atgtgatttg acacaagagg aaatgagaga atatcgtggt actctaactg
     1441 aaccaggaaa agaaagtcct catagaaaca gaactattga agaaaacctt gatttatttg
     1501 aaagaatgaa aaatggtgaa tttaaagatg gtgaaaaaac attaagagcc aaaatagata
     1561 tgtcttctcc aaatataaac cttagagacc caattatata tagaatatct cattcaacac
     1621 accataatac aggagataaa tggtgcatat atcctatgta tgcttttgct catccaattg
     1681 aagatgcaat tgaaggaata actcactcta tatgtacttt agagtttgaa gaccaaagac
     1741 ctctttatga ttggtttgtt aaagaatgtg agatggaaaa tatacctaga caaatagaat
     1801 ttgctagact taatataaat aatactgtca tgagtaaaag gaaattaaaa caacttgtag
     1861 atgagggtat tgttgatggt tgggatgacc ctcgtgttcc tacaatatcc ggaatcagac
     1921 gtaagggata tactgcagaa gcattgcgca atttctgtag tgaaataggg gtatcaaaag
     1981 ttaactctac ggtagatagt caaatgcttg attatttcct aagagaaaac ttacaaccaa
     2041 aggctcctct tactatggga gttttaagac ctttaaaact tattataact aattacccag
     2101 aagataagat tgagatgctt gaaatagaga ataatgctaa agatgaatct caaggaaaga
     2161 gattagttcc tttctctaga gaattgtata tagaacaaga tgattttatg gaagaacctg
     2221 ttaagaaata ttttagattt ttcccaggaa atgaagtccg tttaaaaggt gcttattttg
     2281 taaaatgtac tgatgtaatt aaggatgaaa atggaaatgt agttgaaata catggtactt
     2341 atgaccctga aactaagagt ggctctggct ttacaggacg taaggtaaaa tctacaatac
     2401 attgggttga tgctaaatca gctatcccat gtgaatttag attatttgaa ccattaattc
     2461 ttgatgatat acctgaaaat gaaggtaaac atttcttaga acaaataaat cctaattcat
     2521 tggaaatatt acaaggtttt gttgaaccta cacagataaa agatgctaaa ccttttgata
     2581 aattccaatt tgttagaaat ggattcttta gtatagataa taaatataca actgatgaaa
     2641 aatttgtatt taatagaata gttcctctaa aaagttcttt taaaccaggt aaataatctt
     2701 acaatcaaaa aatgaagact taaaatataa attacttcta ctataaataa acttagttag
     2761 aagtaatttt ttatttgagt cttcatcatt tactttaaat gattaacttt tttcactaca
     2821 gattcccatg acttctattc catcttttat gcattctatg ttaagaggaa aatctggtcc
     2881 tttttctccg tcaatatctg ttataagtga atcttcacat tctatatgca cctttttagt
     2941 tttaaagtat agtatttcat ttccattata ttgatctaaa taatcacctc taagcacact
     3001 tatcaaaact ggtattgatt ttggtattgg cattccttta aaaataatta catccaaata
     3061 tccatcatct atttcagcct tatatgctag atttatattt cctgctgttt tgccattaaa
     3121 aatcaacata agatacatat ctccatcata atacatttcc tcagaagata cttttatctt
     3181 aaatttcctc atatacaatg cttcttctat tccctttata taataagaaa ttcttccaaa
     3241 tgaatttttt aagtctgtgt ttatcctttg tgatacatcc gtaaacatac cagcacttgc
     3301 aacatttata aagtacttat tattgacttt ccctatgtct attttctttg gttttgaatt
     3361 tatgatttca tttatagctt cttttatatc aaatgaaatt tgaagagcat ttgaaaaatc
     3421 atttgcagtt cctgtaggca aaattccaat tggaattttt atgtctaatt ctttcattgc
     3481 atttactact aaatctacag taccatcacc accagctatc aatatgtgtt cataagaact
     3541 atcaacttcc ttaaatgcat tctttacagg ttctttaata gttaatctat agggtatcaa
     3601 taaataattt ttttcttgat ataattctat gatagtgtct aaatttgata atatcttttt
     3661 ttcacctgaa ttaggattgt atataagttt tacttttttc attatttctc cttactcatt
     3721 ttattctttt agtatccaaa atcttcattt aaaaaaatca catgtattct ttctggtgtt
     3781 aattgtcttt ttataagtgt ataactacag catattcttc ctggacttga acagtctata
     3841 catataccag tagtcttaca tccagtattt gtacttaatc ttttacaatt ggctggagca
     3901 gctattgttt ttgttctttt tatagcttca tctacatctt taactatttt atttactcct
     3961 gctataacta ttactttatc aggtccatat atcatagcag caactctatt cccattccca
     4021 tctaaattgt aaagttctcc atcctctgtt atcgcattag tacttgtaaa ataagcatct
     4081 gcagaaaatg actttctata tatttctttc atttcttctg gtttgatatc ttttgcatat
     4141 ctatcaagaa aattatattt ttctcttata aaatcaatca ctcctgtttc aaataaggtc
     4201 atagaacctc cacatcctac tagagaacct gctttaacta tttcttcaat ttttttaaga
     4261 agctcttctt tgttacttac taaataccct ttcatattat tgttttccaa agattttatt
     4321 gttttttcta ttcttttttc attaagccaa tttaaatttt gatccatttt aacttccccc
     4381 ttatttttaa tatgtactta attatttata aactttattt tctttataag tttattttag
     4441 tacatctaaa aaaaaataca atataaaact taaatagttt tacataattt tttccaaaat
     4501 aacttactca atcaaaaaag gaaagtagat gtactattct actttcccta tattgtatgt
     4561 agtatttatt gcctaaaatc tatatattaa ttatatgttt catattatat aatatcacag
     4621 ttaattaaaa tgtaatacta tcttcttttt aaacatataa attgtattat taaatttaat
     4681 atatccaact tcaataaatc ttattaattt gatgaagaag atgctatagc tgcacttgtt
     4741 gctgccatta cacatgcaat ttgaatagca acaacaatat ttaaaaatat attattagtt
     4801 atagagccta tattattctc atttcgtatc aaatctgcat aaattgaagc tacaatagca
     4861 gaactaatca ttactctatt actttttcct aaagaccaat ttccaaagcc tttttcttct
     4921 ttaagtttat ttgaaacatt ttttatctct tctattattt catcttctct ataatctaat
     4981 aatgaaattg ctcctattat tggatatcca taataattca ttttacaatc attttcttta
     5041 aatgcttttt taagttcaat agtcttttta catttaagca tagttctatc atcactaaat
     5101 aaaagaattt gggataatgc ttgtaaatca tttcctttat aaaaaccatt ttcatttaga
     5161 aattcataac atttttctat gtattctaaa tcttcatcta aattttttga atgaattgca
     5221 ataagtgcta tattacaata gtcatcacta gaagttagaa atggatgttt tttcctcatg
     5281 taatcatata ttattttcat ttttgatata catgtaagat aatccatttt ctctctattt
     5341 tcataaacta ttgatgctgt aagtggtaaa tatgtatcgc cccaaaactt ttcactttta
     5401 agttctttgt atatatctaa aatcatctta aaactacttt ctggatttga ttcaaacgaa
     5461 agtagtgttg ccatataaaa catattatat cctctaaaat tagagaataa tccagtattt
     5521 tttttaataa gctctattcg ttcttccact aatctaggac ttattttctt gttcttaagg
     5581 gtaaatgcaa gtgcacaact atgttgtatc aatcccattt gccaagaacc tttaacttca
     5641 tttaatgctt tgtaattttc tattaataag tttacttttc catttataaa cgattgttcc
     5701 atattgccct cctagtaata tatctcattg tactaatagt tttatattta ttatatatat
     5761 attaaatact acaatcaata catattactt caaagaacat cattcttcaa caataatgta
     5821 gccttttttg taatgtcttc ccatattatt gactctatca tagtactctg attcactttc
     5881 attactccat ttgggcacaa atacatctat tgcattagaa ttactaaaat acttactaga
     5941 ccccctatcc ataacagttt ttaattttcc atctacaata atcttagttc caaaacttaa
     6001 gtgattgttt gctacaaaac catcttctaa atcatctcct gtagcagtta tcgctccata
     6061 tccattttct tgattaagac ttgtgtaata actaacttca aaagttatag gtactaactt
     6121 ctttatttta atacctaact gattttgtag tttctccttt tgtactttca acttatactc
     6181 atcactattt ttataatctt ccataccttg attatacttg gaattaaagc ttttacctga
     6241 ctttactata gtatctatat ttactataga ttctttacaa tgatctgcat ctatttttgt
     6301 ttctccattt accaataatg gagttaaaca tacactacat acaacacatg taatcaatgc
     6361 ttctttaagc ttcataaatt ccccccttat tattttgata tattagcata tttaatatta
     6421 tatgtctaca tttagattta aataccacac ccatttgctt taagaagttg tcttatattt
     6481 aattcacctt aaacacttat aaatcctcta tcttagaatc atatagaata aagcttttat
     6541 cattaaatat tttttattaa tcttttaaaa tctaaaacta ctttatctaa ttttctcttt
     6601 tttagaattt aagaattaaa attctataat tttaaaacat attgaattta tttttttgag
     6661 atgatataat tttcttaaga ggtggttgat atgataaaaa atacaaaaca aattacagta
     6721 gaagttaatg aaattttatt acaagaaatg tctaagataa ctaattacga aaataatatt
     6781 ttgacagatt tgataaatga atcaattgta aattatattc ttgaaaacaa aaaacataat
     6841 attgaaaata aagatttaga agaagtgatt aaattttata aaaaagaaaa tactgaaaaa
     6901 cctaaaagtg cagcaagagc tatagctgaa gcaaaaactc atactctgta ttaatcaaat
     6961 aaaaaagttc ctaaaaacta ggaacttttt tatattaata attattaata tatctcaata
     7021 tttatctcaa atctatcaat ataagcatgt ttaatacaat ctattgtata cttatcttac
     7081 cctagaaata atcaaaacaa aaataaaatt atgcccttta tatctaaaac aattaattta
     7141 catgattaat atacaaaaat tctgactttt aaattcgtat acaatatcaa actaaaataa
     7201 tattaatgac aaacccaata taaattaaat tcaaacaggt ttgattttac atcaaactta
     7261 ttgtattaat attaaataac aagacaaaaa tatttataaa aatcacaaat tttacataaa
     7321 actttatatt tgattcattt aactaattat tgtaaaaact atttatttta tgataattaa
     7381 ctacaccaaa ttattcatct tttttaatat ctttaaccca tttttcaaca gagttcaatt
     7441 cttcttcatt tacccccttt ttgaacaagg atttcataaa actctcaata gaacttccat
     7501 gcatattatc taaaaaagtt tttgtttcaa aatgtaaata ctcttcttct ccaataagaa
     7561 ctgtataatg tgtatgtcta tcaatttttc ttgcacttaa gaattgctta tttactaatc
     7621 ttgataaaag tgttagtgtt gtagtttgct tccaattata tagtttttcc atagcttcaa
     7681 tgacatcttt tgatgctact gtaaaatctg aactccaaat aaatttcatg acttttaatt
     7741 ctgctggtgg tatcttagta atagtcatat gattttacac tcctctcaaa cttctacata
     7801 attaaatact atattaatag acttttcgag tcaaaggttt tatttaaaat atctcatcca
     7861 aatttattaa tacaaaaaat ttatattttt attcaaaatt aaacacacaa tctattaata
     7921 taaagataaa ta
//
LOCUS       DAMJWU010000132         7772 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMJWU010000132 DAMJWU010000000
VERSION     DAMJWU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.137"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            307..1500
                     /gene="nifV"
                     /locus_tag="PC460_003294"
     CDS             307..1500
                     /gene="nifV"
                     /locus_tag="PC460_003294"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2602151.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1525..3450
                     /locus_tag="PC460_003295"
     CDS             1525..3450
                     /locus_tag="PC460_003295"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2602152.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREEAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3472..4467
                     /locus_tag="PC460_003296"
     CDS             3472..4467
                     /locus_tag="PC460_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2602153.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4793..5266
                     /locus_tag="PC460_003297"
     CDS             4793..5266
                     /locus_tag="PC460_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2602154.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5300..5794
                     /locus_tag="PC460_003298"
     CDS             5300..5794
                     /locus_tag="PC460_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2602155.1"
                     /translation="MIRKANMNDLESIMQIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5891..6412
                     /locus_tag="PC460_003299"
     CDS             5891..6412
                     /locus_tag="PC460_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2602156.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            6601..6912
                     /locus_tag="PC460_003300"
     CDS             6601..6912
                     /locus_tag="PC460_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2602157.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6940..7626
                     /locus_tag="PC460_003301"
     CDS             6940..7626
                     /locus_tag="PC460_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2602158.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3119 a          761 c         1498 g         2394 t
ORIGIN      
        1 agtaggaatt ttataggttt atttttaaag gttttttgaa aataaatcta tttaaaaatt
       61 aaattaaaat gcaagaaaaa cataataact ttcattttta agaagttttc acaaaaaaga
      121 aaaaatatag tgcaataaat gatacaaagt aatataatag attacaagat atgaaaagct
      181 aaaaaaataa attgcaaatg aaattaaaat gaataaaaaa taaaaaaata atcataagtt
      241 agaggtatca atgaaaaata agacttaatg ttttaaaatt aaaagtatac taaggggggc
      301 attaatatgt gcgttatatc aaaagataga gcaaaggaaa taaaaatagt agatactact
      361 ttaagagatg gagagcaaac agctggggtt gtatttgcaa atagagaaaa aataatgatt
      421 gctgaaatgt taagtgacct aggtgttgac caaattgaag ttggtatacc tactatgggt
      481 ggagatgaaa aaaatgtaat aaagcacata tgtagtagaa atttaaaatc agatataatg
      541 gcttggaata gagcagtaat aaaagatgtt gaagaatcta tttcttgtgg tgttgatgca
      601 gtagctatat caatatcagt atctgacatt catatagaaa ataaattgag aacttctaga
      661 ggctgggttt tagaaaatat ggctaaaaca gttgaatttg caaagaaaaa tggattatat
      721 gtttcagtaa atggagaaga tgcttcaaga gcagatatag acttcttaac tgaatttata
      781 aatgttggaa aacaagcagg ggcagataga tttagatact gtgatacagt aggagttatg
      841 aatccttttt ctataaagaa tgcaatagaa actctttatg aaagaactaa ttttgatata
      901 gaaatgcata cacataatga ctttgggatg gcaactgcaa atgcacttgc aggaatagca
      961 gcaggagcaa attatgttgg tgttacagta aatggattag gtgaaagagc aggaaatgca
     1021 gcattagaag aagttttaat ggcacttaag tgtgtttata aatgtgattt aaataatata
     1081 gatactagaa aatttagagg aatatgtgag tatgtagcac aggcatcagg aagaatactt
     1141 cctacatgga aaccagtagt tggggataat atgtttatac atgaatctgg tatacatgca
     1201 gatggagctt taaaagaccc acataactat gaaccatttg acccaagtga agtaaatctt
     1261 gaaagaaaaa tagtaatagg taaacattca ggaagagctg cagttgtaaa taagttgagt
     1321 gaatatgaaa tgtatatttc tccagaaaac gctaccaagc tattaaatgc tataagagct
     1381 acatctataa gattaaaaag aagtttaatg gataaagaaa ttcttcaatt atattgtgat
     1441 atactagcac atgagaaagg cacaacagaa gaagaagcag ttagaggttc atatatttaa
     1501 gatataattg tttaggagga aaatatggga gataatatag tttataaaat aattaaaaaa
     1561 catatagtag atggagaagc tgttgctgga agttcaattg gtataaaaat agaccaaact
     1621 ttaacacaag attctactgg aacaatgaca tatcttcaac tagaagctat gggaatagat
     1681 aaagtaaaga ctaaaagaag tgtggcattt gtagaccata atatgttaca acaaggattt
     1741 gaaaatgcag acgaccataa gtatatacaa acagtggcag ataaatatgg agtttatttc
     1801 tctaagccag gaaatggtat ttgtcatcaa gtatttttag aaagattttc aacaccagga
     1861 gatacacttt taggttctga tagccacaca cctactgctg gaggtgtggg catgatggct
     1921 attggagctg gtggattaga tgttgcttta gccatggcag gaggagcata ctatataaaa
     1981 gcaccaaaag tctgtaaggt gaatctagtt ggaaaattaa ataatatggt atcatcaaaa
     2041 gatataatat tagaagtgtt aagaaaacaa actgtaaaag gtggagtagg taaagtttat
     2101 gaatatggtg gagaaggtgt caaaagccta tctgttccac aaagagctac aataacaaat
     2161 atgggggctg aacttggagc aactacttca atcttcccaa gtgatgaaaa aacactagaa
     2221 ttttttaaga gtcaaggaag agaagaggca tggatagagc taaaaccaga tgcagatgca
     2281 gtttatgatg aagagattac tataaacttg gatgaattaa aaccattagc tgcaaaacct
     2341 catagcccag acaatgtaga tgaagttgaa aatataggca agattaaaat agaccaagtg
     2401 gcaataggaa gttgtacaaa ttcttcttat gaagacttaa tgaaagtagc acaaatcctg
     2461 aaaggaaata aagtacataa ggatgtgagt ttggtaatag caccaggttc aagacaagtt
     2521 atggagatga ttgctagaaa tggggctttg gcagatataa taagtgctgg agctagaata
     2581 ctagaaaatt cttgtggtcc atgtattggt atgggtcaat ctccaggtac tgactctgtg
     2641 tctttaagaa ctttcaatag aaatttctat ggtagaagtg ggactttgtc agcacaagta
     2701 tatttagtaa gtccagaagt ggcagcagta agtgcaatca agggagtgct tacagaccca
     2761 agagaatttg atattaaatt tacaaattta gatgtaaatg aatttttgat agatgatagc
     2821 atgataatta aacctgctga tgtaggaagt gatgtagaag tagttagagg acctaatata
     2881 aaacctttcc ctcttaatac agagctaagt cagtctatag gtggaaaagt tatattaaag
     2941 acagaagata atataactac tgaccatata atgccatcaa atgctaagtt acttcctttt
     3001 agaagtaata taccgtatct tgcaaactat tgtttcaata ctgtagatac agaatttcct
     3061 cagagagcaa aagacaataa tggtggattt atagttggtg gagataatta tggtcaaggt
     3121 tcaagtagag aacatgcagc tttagcacca ttatatctag gtgttaaagg ggtaatagtt
     3181 aagagctttg caagaatcca caaggctaac ttgataaata gtggtataat tccaatggag
     3241 ttttgtgatg agaaagatta tgaaaatata tctcttttag ataatctgga gataccaaat
     3301 attttagaca atctaggtag tggaatttta gaagttaaga atacaactaa aggaacttct
     3361 tttaaggtca aagtagaatt gtcagcaaaa gaagttgatg tattaaaagc tggtggaaaa
     3421 ttaaactaca ctaaaaatca agctaattaa tatgaaaaga gggaatattg aatgtataaa
     3481 gtgacactta taccaggaga tggaatagga ccagaagttg cgaaagctat gaaaaaggta
     3541 gtagaagcta ctggagtaga aatagaatgg gaagaagtta atgcaggaga ggcagttatt
     3601 gaagagtatg gtactccact tcctgaatat attatagata gtataaaaaa gaacaaaata
     3661 gctataaaag gcccaattac tactcctgta ggtaaaggat ttaggagtgt aaatgtaact
     3721 ttaagacagg cattagattt gtatgtcaat ttaagaccta ttaaaagttt taaggggata
     3781 aaatcaagat atgaagatgt tgatttagtg gttgtaagag aaaatacaga agatttatat
     3841 gctggaatag aacataaaat aggtgattat gcagctgaaa gtataaagat aataacaaga
     3901 agtgcaagtg aaagaatcgt tgattttgca tgtaactatg ttaaagataa taagagaaaa
     3961 aaagttacag ctatacataa ggcaaatata atgaaaatgt ctgatggatt gtttttagat
     4021 gtgtttagag aagttgcatc aaaacatggt gtagaatatg atgatttgat tgtagatgca
     4081 gcagctatga acttagtttt aaatccagaa aattatgatg taatggtaat gccaaatctt
     4141 tatggagata tactatcaga tttaggtgct gggcttgttg gaggtttagg tataattcca
     4201 agtgcaaata ttggtaaaga ttgtgcgata tttgaagctg ttcatggaag tgctccacaa
     4261 atagcaggtc aaaataaggc taatccaaca gcacttattc aatcatcagt aatgatgctt
     4321 agatacttag gtgaatatga aaatgcacaa aaaatagaaa cagctttaga aaaagtattt
     4381 cttgaaggaa gtaaattgac tgtagatttg ggtggctcag cttctactac agagtttgcg
     4441 gatgaggtgt gtaagtatat tgtatagatt ttatttaggg aaaaagtaaa atatagattg
     4501 aaggaaagag gctttctcaa aatagagata agcctttttt attggaatga taaaatatat
     4561 ttgattttat acaaaagaga gttcctcata aacgaaaaag ttaattttgg gatatccatt
     4621 ttaattttaa aattaattga ttaatatgca aagaaagatt aaataaattt attattttta
     4681 attgaacttt caaccaaaat atggtaaaat ttaaaaaagt tgaactttca actaaaaatg
     4741 aatatattga ttatgtgaaa ttttataaaa cagaaactat ttgactttag ccatagtagt
     4801 ttcagtggag gtgtgtttga ttaagacttt agatagtaat atattaagag aagtaggtac
     4861 tttatctaga gcagtaaatt caataaatga tattaagtac aaggaattaa agttacaaaa
     4921 aggacaattt acatttttga caagaatctg tgaaaatcct ggtataaatc ttgtggaatt
     4981 atcaaatatg ttaaaggtag acaaggctac aacaaccaag gctatacaaa aattaataaa
     5041 ggcaggatat gtagataaaa agcaggataa gtttgataaa agaggatata atttaactcc
     5101 aactgataaa tctttggaag tttatgagtt gataattgaa gaagagaata gaagtataga
     5161 gatatgtttt gataatttta cagatgaaga aaaacaagtg gtaactaagt tattagaaaa
     5221 aatgagcaaa aacgtagaaa atgaatggtt taaagtaaag agatagtaaa taaatacatt
     5281 aacttaagga gatattttta tgattagaaa agcgaatatg aatgatttag aaagcattat
     5341 gcaaattata aaatctacag tggaagaaat gaagacttat aataatacac aatgggatga
     5401 aaactatcca ttagaaaaag actttgtttc agatattaag aaacaagatt tatatattta
     5461 tgaagtggat ggagaagtag cagggtttat atgtcttaat tatgaagagc cagaagaata
     5521 tttaggctta aattggtcat ctaataaaaa agcaatggta atacatagaa tggctgtaaa
     5581 tccaaacttt agaaagacag gaatagcatc taagttagtt gattttgctg aaaaattagc
     5641 tgtagaaaac aatgtatcat acttaaagag cgatacatat tcaataaact caaaaatgaa
     5701 tttattactt acaaagtgtg gttttataaa aatcggagaa atgagttttt taggaaagga
     5761 gaaatctttc tactgttatg ataaaatatt ataaagagca tttataatac aagggataaa
     5821 taatagattt tcagcattag taagggcata atataaccta gtgtatgcta ttatgaaagg
     5881 gatgatttat atgattggat ttctaaagaa aagaagaagt attagaaaat acaaggatgt
     5941 tgaagtagaa aaagaaaaat tagataaaat attaaaagct gctcttttag ctccatcatc
     6001 aaaggggctt agaacatggg aatttatagt ggtagatgat aaagaaaagc taattaattt
     6061 atctcaatgt agaactaaag gtggtggatt tttcttaaaa aatgcacctt tagctattgt
     6121 aattattgca gataaagaaa aaaatgatgt ttggatagag gatgcaagta tagctgcttc
     6181 atacatacaa cttcaagctc atgaactagg tttaggttct tgttggatac aagtaagaaa
     6241 tagaatgcat gatgataata tagaagctga taagtacatt agagaagaat taaaagtacc
     6301 tagcaagtac agtgtagaat gtataatttc tataggatat cctgatgaag aaaagaaagc
     6361 atacggtgac tctgatttag actataaaaa ggttcatttt aacaactttt agtatataat
     6421 atgttgtatt aagacattat atttttttct attgttaata tatacttaaa attgtaaaaa
     6481 tatatctgaa ttttactaac ttatctaggg gttatgtaca aggattttaa agataaatat
     6541 agaaattaat gtttataatc gatacataac ttgagtaaca aaatttaagg aggttaaagt
     6601 atgggtatat ttaaaacaaa aatggatgag gactggaaag taaactacat taaagagttt
     6661 aatgaaatga gagattctta tgagagtaaa cttcaaaaaa aacagtttga ggtagatagt
     6721 ttgaaatcag aattggatag actaagaagc tataaaaata gccttaagcc aaaagaaaaa
     6781 caaattactg atgatgatat aaataatata aagagtttaa gaagagatgg cttaagctat
     6841 aaagaaattt caaatcaaac aagttggagc aaagcaactg taagtagggt attaaatgga
     6901 ctttacgatt agcatataca tggggtgatt taaattggga tgaagtatag ttacaaaaaa
     6961 tgcataatag attctatttt attgatgttt atagttcaaa tattaaggat gattttgaat
     7021 tatgtacttc taagtcaatt tgaatttact cttgaaaatt ttaatattat aaacttaatt
     7081 tcttttacat tagttggttt atcgttaatt ttatttttaa aagataattc tttgtataac
     7141 aaggttagaa atagaaaaat cacagaagct tttgaagaaa ataaaaacaa tatacttatt
     7201 gaaaagtgta agctgatact ttttgttgtg gttttatctt tagcaataat tgtaacttat
     7261 tgtactaagg gatatgtact ttttaatgta actatgatga ctttgtctgt attgatagtc
     7321 ccaatatttg aagagttatt ttttagagaa tatatatgga actatctaag taattttata
     7381 aagtcaaaga gtaaaattat ctgtataaca tctatactat ctggaattta taatatagga
     7441 tacatagatg ttataagaaa ttatgtaata ttgtataaca attcaagcta cacctttgaa
     7501 gttattattt caaaaattat gataggtact gtttttggta ttgtacttgg attagttaag
     7561 tataggttta gagatgtagg gttttgtata ttattgagga gcttatttgc tatatttatt
     7621 aggtagatag tataatcggt ttataaggtt gttttaaatt taattttaaa tcaaccttat
     7681 tttatttgtg ttttataaag cacttatata ttaataacta ataaatttaa taaaaattat
     7741 ttgaatattt ttaaaatata taaatatgta tt
//
LOCUS       DAMJWU010000133         7727 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMJWU010000133 DAMJWU010000000
VERSION     DAMJWU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.138"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            230..1531
                     /locus_tag="PC460_003302"
     CDS             230..1531
                     /locus_tag="PC460_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2602159.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1607..1882
                     /locus_tag="PC460_003303"
     CDS             1607..1882
                     /locus_tag="PC460_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2602160.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3325..3555
                     /locus_tag="PC460_003304"
     CDS             3325..3555
                     /locus_tag="PC460_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2602161.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3548..4129
                     /locus_tag="PC460_003305"
     CDS             3548..4129
                     /locus_tag="PC460_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2602162.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSI"
     gene            complement(4704..5381)
                     /locus_tag="PC460_003306"
     CDS             complement(4704..5381)
                     /locus_tag="PC460_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2602163.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5570..6934)
                     /locus_tag="PC460_003307"
     CDS             complement(5570..6934)
                     /locus_tag="PC460_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2602164.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7062..7583
                     /locus_tag="PC460_003308"
     CDS             7062..7583
                     /locus_tag="PC460_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2602165.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3013 a          870 c         1338 g         2506 t
ORIGIN      
        1 atatattaat aattggtagt aaagtatatc aaataagata ttgctattat gtagtaaatt
       61 atataaaaca atgtatcaga ataaatagta tattaataat ttgtagtata tcatgacgaa
      121 taatacagtc ataacaaata gtgagtcata acaaataatg tatttataat tgataatgta
      181 tttaaaaatc aaaatataaa aacttataag gaaagagaga ttaatttata tgggtaaatc
      241 aaaagatgtt gaaatagaga agtctatttt aggcacaatt cttttagaca ataaattaag
      301 ctataagcta gatgaattaa atgaaaacat gtttatgaat gatatatgtc ttgagatatt
      361 taaaattatg aaagaactta aaaaagaaaa catagtgatt gatgtggcca ctgtgaaatc
      421 aaagatagat agaaaatctc tagcaataaa aacgagtgat gtgactaatc taattacttg
      481 gggtcagaat tttggtcttg atggtcatat taaaatttta aaagaaaatt tagctagaag
      541 aagcataaat caaaattgcc aaaacctttt acacagccta aaccttggag aaaatatcga
      601 tacttgtatt tacaaatttg aaagtaatat aaaggagatt ttggacaagg atacttatga
      661 aaatgatgat gtaaatagta tagcagggaa ggtgcttgat tttttagaaa acaaaaaaga
      721 tattggattt aaatttggta taaaattact tgataccact ataggaggtc tttttaaagg
      781 agagctaacc acaatagcag ctaaatcggg ggttgggaag actgctttgg cacttcaaat
      841 aatgttaaat tcatttaagc aaggtaaaaa aactttattt ataagtagag aaatgaccag
      901 tgagcaagta tttatgagaa atatatgcag agtaactggt gtaagtacaa ggaatatgaa
      961 atctaaagaa atagatgaaa atgattggaa acttattgta aatgctatag gtgatttaag
     1021 cgaaaataac ttaatttata ttaatgataa gatagataca attagtgcaa ttagaaagag
     1081 gataaggcag gtaaagccag atttattaat agtggattat gtgcaactcc ttacatctca
     1141 gaaaagcatg gataagagag aaagggaggt agctactttt tcgagagaac ttaaaaatat
     1201 gactttagat tttaatatac ctgtaatcca attaagtcag ttaaatgatg aaatgaaaga
     1261 ttcaagacct tatggagata gacctatgag ggatagcaaa gccatctacc acgatagcaa
     1321 caatgtggtt tatatacatc aattaaaggg gagtgattat gaagaagcag tgagagatat
     1381 tggagaaagt gaggaggcag ttagagcatc agaatacagg ggaattaaga tggttgattt
     1441 aatagttgcg aagtgtagag atggacagac taggcataag catttttgtt actttgggga
     1501 taagttacat tttcaagaac taaattatta ggtcaataaa gtagtaataa caagcttatt
     1561 aaaagtcggg aaatagcaaa tagattataa aatatgaggt gaaaatatgt tggaattaag
     1621 aaaaattata gaattacttg aagaacacaa gatagaagta agtattccaa aggagttaca
     1681 taagtatagt ttattggaat accttgaatt gtgtcaacaa agtgggggaa catattttta
     1741 tattaaaaca ctggaagggg ctttaaaaga ggctagaaat gagcttatga ttaaattgtt
     1801 taatgggaaa aattatatgg aattagcgag gatgtttgaa atgagtgtta ttaatgtcag
     1861 gcatattgtt agaaaaaaat agatattttt ggagaataca agtgtgatat acttctgaaa
     1921 tagaagttat tatagttgaa ataaaattgt ggttaataaa ttaaagttgt gtataagttt
     1981 aagattatag aggagtataa gtgtgattaa cagcatgatt ttgtgaataa aattgaggat
     2041 atatatgagt taaatgtgtg attaataagg taaggttgtg tataagatta tgagtattta
     2101 aaaggagggg ctataattaa tagtgtagag ttgtgtgttt gcttaagatt atataataat
     2161 ttaatagatt aaactatact aataaggtaa aatcgtgtgt ttagttaaag cttattataa
     2221 aatcttgata tgtgattaat gatgcaatat gtgaataaag ttaagattat aatagattta
     2281 acctgtggct aatagaataa aattgtgaat aaagttaaga ttataatagg tttaacctgt
     2341 gattaataga gtaagagtgt aaatagagat aaacttcaat atgagcttaa cctatgatta
     2401 atagagtaag agtgtaaata gagataaact tcaatatgag cttaacctat gattaataga
     2461 gtaagagtgt aaatagagat aaacttcaat atgggtttaa cttgtgatta atagagtagg
     2521 gtgtaactaa agataaactt caatatgagt ttaacttgtg attaatagag taaggtgcaa
     2581 ctaaagataa acttcaatat gagcttaacc tatgattaat agagtaagaa tgtaaataga
     2641 gataaacttc aatatgggct taacttgtgg ttaatagagt aagagtgtaa ctaaagataa
     2701 acttcaatat gggtttaact tgtggttaat agagtaagaa tgtaaataga gataaacttc
     2761 aatatgagct taacttgtga ttaatagagt aagagtgtaa atagagataa acttcaatat
     2821 gggattaact tgtgattaat agagtaagag tgtaactaaa gataaacttc aatatgagct
     2881 taacctatga ttaatagagt aagaatgtaa atagagataa acttcaatat gggtttaacc
     2941 tgtggttaat agagtagggt gtaactaaag ataaacttca atatgagttt aacttgtggc
     3001 taatagaata agattgtgaa taaagttata gttttagatg ggtattaacc tgttactaat
     3061 agaatatgat taggtgtaaa attatagagc tgtataagaa aatattatag atatattaaa
     3121 tattagataa taaatattta tatagataca caaaagaaaa ggcgatatga agctaaactt
     3181 aatgacttca tatcgttttt tatttgaaac tcctttgtat ttagagtgtg atttttaata
     3241 ttgaggtttt atgtgattaa gtcacagata aaaagtaagt atcttgatat aattcttata
     3301 gattaataat atagggaggt tattatgtct aataaaaaaa agaagtatgc aaatatttta
     3361 gcaaatgagt gcgttgcttg tggtagctgt ataaaagcat gtccaagaag tgctatctca
     3421 gttccatgtg gcattagtgc aaaaattaat agagatttat gtgttggatg tgggatttgt
     3481 gaaaagatat gtccagcatc tgtaattgag attataacaa tattaaaaga ggaagaaggt
     3541 aagtgtcatg aataataaaa aacattggta tgactattta tggatttttt cttcaatata
     3601 tttgatattg ggatttgtaa atattatttt cgcttggatt gggctaattt gtttctttgt
     3661 tccactagct atttctattg ttaagggaaa taaagcttac tgtaataagt attgtggaag
     3721 agggcaactg tttaatttgc ttggaaataa attgaagctg tcaagaaata gagatattcc
     3781 taaatttatt agaagtaagt ggtttagata tgggtttttg acatttttta tggtaatgtt
     3841 tgccaatatg cttttttcaa cttatttagt gtttgaagga agtagaaatt ttaaagaagt
     3901 agtgactttg ttgtggacta taaaaatgcc ttggcaatgg gcgtatcatg gaactttagt
     3961 ttcaccttgg gtggtacagt ttgcatttgg tttttatagt gttatgctta catcaactgt
     4021 tcttggtttg attactatga tattgtttaa acctcgttca tggtgtgttt attgccctat
     4081 gggtactatg actcagatga tatgtaaggc aaagagtaag tctatataga aaggctttat
     4141 gtgatattta cagttttgtt agagatgagt agtaggtttg aatatatatg acaaatatat
     4201 gagtataggg gcatagattt agtttgtgta aggtaaactg atttatgctt cttttttatg
     4261 tcttagatta ttggctaagt ggaaacaatg aatttagata ttaagaatta ttcagttatt
     4321 tgaagaaaat agaataataa ttaacattat atgaatggat attcattaca tataagccag
     4381 ttaatagcaa gatttattta atatgaaata ggtcaagtat ttaaactaaa taaggtcatg
     4441 acaattaata taagtataga tataaaaaga ttagaagtta taacttataa aaggtattct
     4501 atataaatag tatgtggaaa atagtcaaat taacttatta gaaacaaaaa atatacataa
     4561 aaaaataaat gtaaatatca aataagatac tatataaaaa tagatataag tatctttttt
     4621 attattgaaa ttatgaaaat atatttgatt tcacacaaaa aagagtgcct catgaactaa
     4681 aaagttcatt ttgagacaca cttttacagt gtcaaagaac gaagtttatc cttatccaat
     4741 acttttatac caccacgaga taaagacaca ataccttctc tagcaaaata tttcaacatt
     4801 ctcgatacaa cttctctggc actacccata tacttagcaa tttgctcatg agtcatattt
     4861 atagtgctac tgccattttt agctatctca tcaattaaaa aagatgccaa acgcttatca
     4921 aaactcataa aaagaatttg ttgcatagcc cacataacat cagaaaaacg ttcggtagct
     4981 aatttatatg aaaataactc tgcatgaata ttttctgtag ataattttgc aaaaacagag
     5041 gaactaatct gtataatatc gcagtccgtt tcagcatcta catagacatc aaaagttatt
     5101 gtttttagaa tacaggaagc tgataaaata catacatctc catcatcaag tcggaacagt
     5161 gtaacctctc gaccttcatc tgaaagtatg tatgtacgta ctgttccaga ctttataatc
     5221 aaaataccaa tacagtcact atcaccacaa tgtactggat tccctttttt gtaatgcgag
     5281 gtatttgctg aagaaattaa taattctttt tgtaagtcag taagtttatc ccaaaaaggc
     5341 aagctatgtg taagtatatc gatttcattt tgtgaaatca tacaatatct cctttcttaa
     5401 attgttataa tcccatgata caagtgaata gataaattga caagtagttt gttaatagga
     5461 ttatttagtc agatagtaat agtatgattt atgtagaaaa aatatgataa tatatattct
     5521 tgctattaat gagaaggtaa tatgttttga tgatagtagg tttgtataac taatgcatac
     5581 cgtttatttt taaaagatta agttccttat gtaaccttat agacataaaa ccagcaatta
     5641 tagcagccat caaatcagct attggttgtg catataagat accatttaac ccatagaata
     5701 caggaataat caaaattata gggataaaac agacaccttg tctacacata cctaaaaatc
     5761 caccttcctt tgcttttcct agtgcgagga ataatgtaga atacactgta tagaatccaa
     5821 atagcataaa tgaaaaccca tttgccctaa gagctttact accattttct attaatagaa
     5881 tgtcaccact tgtaaattgt gaaattatag attttgggaa gagtatcaat ataagaccaa
     5941 gcactacaca aaatattgta gtccataata tggaagtttt tattgctata tgtaatctat
     6001 cgtagttctt tgaaccaaaa ttataaccag caattggttg gaaacctttt ataaaaccaa
     6061 atacaacaag actacctaaa gacgttattc gtgttacagc acccattcca gctatcacag
     6121 aatcaccata ctttgtagcc tccatattag ttaatgtaat ggcaagacta gtcaataatt
     6181 gaaatattaa ggtaggtatg ccaattttta ggatttctga aaaaatttct tttgaaaaac
     6241 agcattcgtt aattctaaaa ctaaatatac ttttcttgct taaaatatat cctacataaa
     6301 caagtgttga agcaatctgt gagatagctg ttgcaataga agcacccctt acccccagac
     6361 caaaagtata aatgaatatt gggtctaaaa tcatattaag caaagcacct aataacatag
     6421 cacacatagt tgttttagca gctccttcac ttgtgacaat attagtcata gtgacattga
     6481 atatattaaa aatagatgac acgacataaa tgctagcata agatattgca taaggcataa
     6541 tactttttgt agctcctaac aattttaaaa ttggattgag aaaaatcata atcacaataa
     6601 taactaatgt acccactata atactactgt aaatagatgt acttaccact tttcctgctt
     6661 tttcattatc accatggcct aaaagtctgg caatatatga agcagctcca tttccaaaca
     6721 ataatcctaa tcccacaaca agctgaccta atggaaatgc aacggatata gctcccattt
     6781 gacttgttcc aagtccaccc acaaagtatg catcaaccaa attgtataat gcagttatca
     6841 tcatacctat catggtaggt aatcccatag ataacaatgc ttttgagatt ggggcagacc
     6901 ctaataattc aattcttttg ttactttctt tcatacttaa cactcctctt ctattgacat
     6961 taagtctaaa ttataatact ataaattata gtaagtatgg aaaatagtac tataatttat
     7021 agtatttgtc aatagtaaga aaactaaagg agatgatttt tatggctaca atagatttaa
     7081 ttgtattggg aatagtaaag caacaaccca agagtgctta tgatattcaa aaggcagtgg
     7141 aatacaggaa tatatcacgt tgggttaaaa taagtacacc atcaatttat aaaaaagtaa
     7201 ttcaattaga acaaaaggga tatattgaaa gtaaaactgt gaagaatgga aaaatgccag
     7261 aaaaagcagt ttattcatta actgaattag gtgagacaca ttttttagat ttaatgtttg
     7321 atacatctag caaaccaata aatattcttt tagattttaa tgctgttata gtaaatttaa
     7381 atagccttac accagaaaaa cagaaattat gtctcaataa tattgaagag aatgttcaag
     7441 agttaaagaa aactattgag gaaaatataa gtataaagac agaagtacca gacacagcaa
     7501 aagcagtgtt ggagcaacag ttggtgctag cacaagcatt ggaaacctgg ataaattctt
     7561 taaaagataa tatgggaaat tagaattaga gaagctaact taataatcat taaacaaaaa
     7621 ccagcatatt taataatgag atattagaaa aaataaaaca aatgatataa actgaaaagt
     7681 atatttaaaa taaagctcac ttaattgagg gagaatgtta aactttt
//
LOCUS       DAMJWU010000134         7722 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMJWU010000134 DAMJWU010000000
VERSION     DAMJWU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.139"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(224..2503)
                     /locus_tag="PC460_003309"
     CDS             complement(224..2503)
                     /locus_tag="PC460_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2602166.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2774..4228)
                     /locus_tag="PC460_003310"
     CDS             complement(2774..4228)
                     /locus_tag="PC460_003310"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF2602167.1"
                     /translation="MKLNDIIQGLDIINIKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4392..5261
                     /locus_tag="PC460_003311"
     CDS             4392..5261
                     /locus_tag="PC460_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2602168.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5474..7546)
                     /locus_tag="PC460_003312"
     CDS             complement(5474..7546)
                     /locus_tag="PC460_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2602169.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVSTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         2618 a         1286 c          823 g         2995 t
ORIGIN      
        1 ttactatcta aagtattttg ctataatccc atatactctt acctcaaaaa cattcaaatg
       61 taaaaaatac tacatattaa taaactttgc ccaataattc ctaaaataat atcctaacaa
      121 tgatttatgt ttttataata gtatattttt acataaaaaa gtgaacattg attaacaaag
      181 ttcacttttt tataaatttt tatgttataa ttcacaattt tatttaattt gtttcttttc
      241 tatttaaact atatctatct aatattaatt tttcatattc ttctattggc attggtctag
      301 caaataaaaa tccttgggcc atatcacaac ctataaacct taaaaactct gcttgctcta
      361 cagtttcaac accttctgaa acaactttta tatcaagttg ctttgccatt tgaacaatac
      421 ttgaaactac aatttgactc ttttttgcat ttcccactgt tataaagaat tgcctatcta
      481 atttcaatac atcaattggc atatctttaa gcatatttaa agaagaataa ccagaaccaa
      541 aatcatccat agaaatttta aatccaacag atttcaaagt ttgcattatt ttaaataata
      601 tttttgtatt gtcaaatatt gcactttctg tcaattcaat ctcaattaaa tttggttcaa
      661 tttcatattc ttttgttatt ctttcaagct cagaaataaa gcctggattc tcaagatgta
      721 ctcttgataa gtttattgat ataggaaatg tggggaaatt ttcattttcc caacgtttaa
      781 aatttttaca aacttgggta aatacaaaca tatccaattc tgttataaag ccatttttct
      841 caaaaatagg aataaataca cctggactta ttagaccttt tttaggatgt tgccaacgta
      901 caagtgcttc agcacctaca attttagtag ttgataaatc aaccttaggc tgtaaataaa
      961 ctttaaattg ttcatttttt aaagcatcat gcatttcttg ttcaatatca cgttcttcta
     1021 tgagagtttc ttttaaattt tcagcataaa tctcataaac ttttaaatat tttcttttta
     1081 ctgtagtttt tgcaatcata gctttgtcaa gacaggtaga aatatttgtt tctccctctt
     1141 caacaaaata tacacctatg gatggaatta aattgagttc tactcccaaa cttgttttta
     1201 tcattgacaa ttttttacgt ataagttcac atatttctat aattgactct ctattatttt
     1261 tcttttcaaa tattatagca aaattgtcat ttgaaattct tgaaaataca gattgctctt
     1321 taaatatatt cttaattatc tctgctattt cctttaatac tctatctcct tcttcatacc
     1381 caaacctatc atttactagc ttaaacttat ctatatcaaa ataaattaaa actagattct
     1441 ttttctcatg agaggataaa aattttcccc cttcaagatt aaatttattt gaattgccaa
     1501 tacctgtaac gttatcttta aaagcaaatt cttccaattt ttccttactc tttttatgta
     1561 taaatagtat ataaattgtt aatgccgtaa agattataat caagactaca catgctgata
     1621 atgtaagttt tataatagca tatgatttct cagatatagc agttttagga acttcagaaa
     1681 aaagatacca atcattgatt cctattgggg cataacttaa ataatatcct tttccaaaca
     1741 tactataact taatgaacct gatttaaagt tttttatatt tgacttcata ttgttgatac
     1801 tagattttga tattgaatca cttctgccaa tgtaagaaaa taaattttct ttagtagagt
     1861 tggcgttttt actgcttgga tttaaaacaa tatcaccatt acttctagtt atataagaat
     1921 atccttttcc attaaatgta ggtacagaaa gtattttttc atacaacttc gtactatgtg
     1981 tggcaaatat aactccttta acctcattat ctttataaat tggtacagaa tatacattaa
     2041 tattactatt ggtattttta tcatcttcta ttacatcttt taatttgcct gttataacca
     2101 cttctccttg cattgatttt aagaaataat ctcttttaga gaagtcttct atataaccat
     2161 ctgtagtgta tgctgtacca tttggtaaaa taactcccat tcttttaaat gaactattaa
     2221 tagcttgtct ttttagtata gaaaggatat tatctacttc aaaagactct tctccttcaa
     2281 taaatattga aattgactct agtaacttta tatctccatt aatttgtttt tttaacacat
     2341 tgacactttg atttgctatt tctgaaagat attgattcgt ttcttcacct aatgtcttat
     2401 tcaaccttga accataaatc aaacaggaaa ttgtaattac taagatactt atgacagcaa
     2461 ttatggacaa accataccta ttattttttg catcacttaa cattatgctt cctgcctttc
     2521 tcgaatctat gtatgtataa tcattatctt atgacaatta ttgtaaacat tttataataa
     2581 tacttatttt tccatattta ctgtaattat acaaaattat aaactttatt acaacaataa
     2641 attaattaat atagatatta gtgtttatat tattgcatta tctattttac aattatttac
     2701 atataaaaaa atgtaatatc aaagtgaagc ttgatattac atttttgttc ttgtattttg
     2761 tttaattttt atgctacatc ttacttaaac actcatttgc aacttcttta tcatcaaaat
     2821 gatgcttaac tttacctata atttgataag tttcatgtcc cttacctgtt ataattacaa
     2881 catcatcctt ttttgccata cttatagctt ctgctatagc ttgttcccta tcaacaacaa
     2941 catggtaatt ttcttttttc ttgtcaattc cttcaagtat atcttttatt attgcctctg
     3001 gttcttcagt tcttggatta tcagaagtta taatacataa atcagaatat ttttgtccaa
     3061 ttgctcccat caaaggtctc ttttcagtat ctctatctcc accacaccca aacacagaaa
     3121 ttatttttcc ttctgcaaat tgttgagttg tctttagtac attttcaagt gcgtcaggag
     3181 tatgagcata gtctacaatt acgcttatac ctttatcatt tggaactgtt tcaaaccttc
     3241 ctgatactcc attagaaagt ctaaggcctt ctttatatat tggtttaggt atgccaagta
     3301 catagcaagc agctataact gctaaagtat tatatactgt aaacattcct ggaactggta
     3361 taaatattac ttcttcatat gaaggtgtta ttaatctata agatactcca tccgaatctg
     3421 attttatatc tctagccata aaatcagcct tagtatcaac accataagtg taacaaggta
     3481 catttatacc ttttatattt tcatatatct tttttccacc ttcatcatct atgtttatta
     3541 tatttgccat agtagtctta aaaaataatt tttcttttgc ttttctataa tcctctaaat
     3601 ctttatgaaa atctaaatgg tctggtgtta aatttgtaaa tatgcctagt ttaaagtctg
     3661 tttcatcaac tctatttaat gccaatgaat gtgatgaaac ttccatcgca cagtagtcac
     3721 atccattatc caacatatta ttaaaatgat attgtaaatc tatactttct ggagttgttg
     3781 aattggaaac aatgtctttt tctccatcaa aaatttttat agttcctata agtccaactt
     3841 tattcttatt taaagttaat atctcattta gtatagtagt tatactagtt tttccattag
     3901 tacctgtaac tcctataacg ttaaattttt gagatggatg attgtaaaaa ttatcagcta
     3961 cttttgccat atcttttctt gtatctttta cctttataaa tgtacatcct tttatttcta
     4021 catcttcttc aacaagaaat gcacttgctc ccttttctat ggcatctttt atatacttat
     4081 gaccatctga cacaaatcct tttatacata taaataatgt accttttgta acctttcttg
     4141 aatcatattg tacattatta atatctatat ttaattcacc cttgatattg attatatcca
     4201 acccctgtat aatatcattt aatttcataa atgctttcct ccctttaaac tctttctgct
     4261 aaaattacac tacatataat attatattaa gaatttttta ctatgtgaat ggttaaaaat
     4321 gcttgaaaca ttgcaaaaaa tgctctataa ttttctataa tataactgag gaaaggtctt
     4381 ctataaaaac tatggataat aaaatcgggt acttaaacga taactttaaa atatttaata
     4441 ttagagataa aaaagatata caatttgaat accataatca tgattttaat aagataatta
     4501 tatttataaa tggaaatgta acttattttg ttgatggtat tccctataaa ctaaagcctt
     4561 gggatatact ctttatcagc aataatgaaa tacataaacc agttatagat tcaaatgtct
     4621 tttatgagag aattgcaata tggattagcc cagaatttat gaaaaaaaat agtgatgcta
     4681 gtagtgactt agaaaaatgt tttaaaatag ctacagagaa taaatgcaat agattgcgtt
     4741 taaatataca tgatattgaa tctattaaga gttttattga gcaaataaat atttccgaat
     4801 ccaatactga atttggaaat gaaatattga gaaatgcatt atttatacaa cttatgatat
     4861 tcttaaacag aattttccta aaaaatgaaa atatggaatc aacagaagat gttttttatg
     4921 atgaaactat taagaaagta ttaaactata taaattcaaa tatagatgat aagctttcaa
     4981 tagatagcat atcgtctgaa ttttttataa gtaagtatta tcttatgaga aaatttaaat
     5041 ctcatactgg aacgtctata cacaattata tagttcaaaa aagattaatt ttggcaaagt
     5101 cactaataac caatggtcac cttatgagtg aagtttgtaa taaatgtgga ttcaacgatt
     5161 attctagttt tgtaagggca tttaaaaaaa actacggggt atctcctaaa aattatataa
     5221 acaataaaaa tcctttggat attactcttt tagatgaata gataatttat gtaaatttta
     5281 ttttaataaa acttgaagta ttagtgtatt tatcttacta atacctcaag ttttttaatg
     5341 ttttaaaata tatcaaaata taatgatata tatttctatt acttaaaata tatattaaat
     5401 aaattgcatg ttaactgaac ctatttatat atttattaaa tcccacataa cttttagtta
     5461 cactatcata tagctatcct attttttcaa tatcatctct taatatactt actctattct
     5521 cctcttttgc tgtgcttact ccttttttaa tgcttaattt ctgattttgt tctaaattcg
     5581 taaatactac aggacttatt gttgatagac catttttttc aatatattct aagtccattt
     5641 taactaattt ttctcccgct tttacttcat cccctacatt cacaaatact tcaaatccat
     5701 ttccttctaa atttacagtt tctatcccta aatgtataag tatttctact ccactttttt
     5761 gggattttat tattattgca tgcttagttg gaaatataac tccaatagtt ccatcaactg
     5821 gtgaataaat caatccatca ctagatttaa ttgcaaatcc atcccccatc agtttacttg
     5881 aaaatacaga atctggcact tcttctatat ctaatatctc tccatttgtt aatgaatcaa
     5941 tcttttctac taaagcattt tccatatttg caacttccat attttttttc actttatttt
     6001 tatcaacatt tttagtaaca gtactttcaa cctttgtttt tccctctatt atatctttta
     6061 tatcagactt tattgaatcc gattttggtc caaatatagc ttgaacattg ttgccaataa
     6121 ccataacacc tgctgctcca agtgatttca atttttcttt atctacttta tttatatcat
     6181 ttacaaccaa tctaagtctt gttatacacg catctaggct ctttaaattg tctttatttc
     6241 ctaaagcaat caaaatctct gatgctaatt cattcttaga catttcaatg acttcagcgt
     6301 tagtttctga ctcatcttct cttcctggtg tctttaaatt aaattttctt atcataaatc
     6361 taaatacact atagtataat actgcaaaac caatacctac caagatagcc aagtaccatg
     6421 gagttctatt aggaagaaca ccaaataata agaaatctat taatcctcct gaaaatgtga
     6481 atccaatttt aactcctaat aattgcataa ttgcgtatga taaacctgaa aataaacaat
     6541 gtattgcaaa taatactggt gcaacaaata aaaacgcaaa ctctattggc tcggttattc
     6601 ctgttaaaaa agaggttaat gctgctgatg ccattatacc tgctacaaat tttttctttt
     6661 ctggctttgc ttcatgcacc atagcaagag ctgctgctgg aagaccaaac atcataaatg
     6721 gaaatgcacc tgtcataaaa gtacctgctg tgaaatttac tccatcttta agttgagcaa
     6781 agaatatctt ttggtcaccc attacaagct caccagcttt atttacatat tctccaaatt
     6841 gataccaaaa tggattatgg aaaacatgat gcaaaccaaa tggtattaat agtctttcca
     6901 aagaaccttc cataaatgct gctagtgttt gatttgcttc tataacattt tttgagaaac
     6961 taagtaatcc ctcttgtatt ggaggccaaa taaaagacaa tataattcct attgctatag
     7021 caactaatga agtaacgatt ggtacaaatc ttttacctgc aaaaaaacct agatactgag
     7081 gtaattcaat tttataaaac tttttataaa gataagcagc cactatccct ataataatgc
     7141 caccaaatac ccctgtttga attgttggta ttccaacaat atttgcatac atagaattgt
     7201 ctaccatatc ttctgtaaca cctgccataa cacccataga tgtattgaga attaaaaaac
     7261 ctactatggc tgccaatgct gcaacacctt ctccttcagt taaaccaaca gctaccccaa
     7321 ctgcaaatat aagtggtaag ttattaaata taatttgacc tgaactttcc attatactta
     7381 ttataagttg aaatgtagga ttcgccaaag ctggaaaatg agccaacaat gcgtctcctg
     7441 ctaaagcatt actcacgcca agtaatatac ctgctgcagg aagtaatgca actggtagca
     7501 ttagagattt tcctactttt tgtaaaacac caaatacttt tttcatttac ttttctctcc
     7561 ttattatttt atattttttt aaataaaaaa gacttaagtt tataaaaagt tacattagta
     7621 tacttaattc taaaattaaa cttatgttaa tataaataaa cctatattaa cctaatttca
     7681 gaattaaaaa taaactatta taattttttt ataaacataa gt
//
LOCUS       DAMJWU010000135         7688 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMJWU010000135 DAMJWU010000000
VERSION     DAMJWU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.140"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            193..1596
                     /locus_tag="PC460_003313"
     CDS             193..1596
                     /locus_tag="PC460_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2602170.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1687..1927
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1970..2668
                     /locus_tag="PC460_003314"
     CDS             1970..2668
                     /locus_tag="PC460_003314"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF2602171.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2691..3692
                     /gene="trpS"
                     /locus_tag="PC460_003315"
     CDS             2691..3692
                     /gene="trpS"
                     /locus_tag="PC460_003315"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF2602172.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4344..5911)
                     /locus_tag="PC460_003316"
                     /pseudo
     CDS             complement(4344..5911)
                     /locus_tag="PC460_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(6247..6429)
                     /locus_tag="PC460_003317"
     CDS             complement(6247..6429)
                     /locus_tag="PC460_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602173.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
     gene            complement(6818..7564)
                     /gene="cdtR"
                     /locus_tag="PC460_003318"
     CDS             complement(6818..7564)
                     /gene="cdtR"
                     /locus_tag="PC460_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF2602174.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         2748 a          915 c         1125 g         2900 t
ORIGIN      
        1 attaaagtgg taaaactttt aaatttatca tttttaataa gatttgattt caaagtattt
       61 aaggtttatt attaaaactg tttttgaata tgcaaatatt ttgaaaaata tcagtttaat
      121 aataatgaat ctattttatt aattttgaaa ttaaatttaa aataattaat agagagggga
      181 aattaaatta ggatggaaaa gaaaaaaata ggcctatgga gtttggtatt tatgaatgta
      241 tcagctttat atggtataag atggatagct aaatctacag catcaagctt tggtttagga
      301 ttaggtgcaa tacctatgtg gttattattc tcatttatat actttgtacc aggagcttta
      361 atttgtgctg aattagcttc tacatatcaa aaaggtgatg gaggtcttta cgattgggtt
      421 aaggaagcct ttggagaaaa gtatggattt ttagtttctt ggttaaactg gacagctaaa
      481 atattttggt attcttcatt tttaactttc ttagcagtaa atgtttctta tacaatagga
      541 aatccagccc ttgctgataa taagatgttt gtacttatat tatctttaat aatattctgg
      601 gcattatctc ttatagcaac taaaggaata agttttacaa aaatatttac taatacaggt
      661 gcattaggtt caacaatacc aagtgctata cttataatat tttcatttgt agcagtattt
      721 gtattgaaaa aacatgatat agcttctaca tacacaatag cttcaatgac tccaaaactt
      781 aatatggatg catttgttgc tatatcagct ataatgtttg gtttagcagg agcagagact
      841 gctgctaact tcataacaga aatagataag cctgaaaaga acttccctaa ggcaatatta
      901 atatcagctg gaatagtagc ttcattatat gtattaggtt caattgcaat aacaatgata
      961 ataccaccag accaaattac tgcttctaaa ggtgtattag atgcactttc tgctgtttgt
     1021 gcgtctttag gaattggttc ttggttggtt caattaatag cttttggtat agctttttca
     1081 gtattaggag caatagttct ttacatagca tcaccaataa aaatgttatt tggtagtgtt
     1141 aaaaaaggaa tatttccaga tagtttagta gaggtaaatg aacacaagat accttctaaa
     1201 gcagttatac ttcaagcaat aatagttact ataatactat tggtgactac attaatgcct
     1261 tctgttgatg ctatatacaa tgttcttgta actatgacag cattaactgc attattccca
     1321 tatgtattgt tatatgcttc ttatattaaa ctaagaaaag agagaccaga tgaaattaga
     1381 ccttatacta tggcaaaatc aacaagtact tgcctaggtt tagcaaaaat ggttttagtt
     1441 gtaactgtag tgggtatatt attatctgca gcaccagtta tgcctacatt tgctgaaaat
     1501 gttatatatg agatagagat gattggtgga ggtcttctag ttatattatc tggtctatgg
     1561 ttatggaata gatatgaaaa gaaaacagct aaataaaatg tattttaaaa atttcttttg
     1621 gatattataa tatatagttg acaatttata gtgtaaggaa tattataaaa taaagaaatt
     1681 aataaattaa atcctgagat aagaaatagt aaataaatat atttttgcac agagaattaa
     1741 ggatgctgag aacttaataa aaagtattta ttgaatgggt ctttgaggag taaaatgaaa
     1801 tgtaatttaa gattacagag tatttgatat cgtgtatgca cgttatagca tatattaaga
     1861 tatatatatt caattttgag tgtatataaa tttaggtggt accacggaaa atatatccgt
     1921 cctattgatt taggacggat tttttaatat agaaaattta ggaggaaaaa tgaacgtaat
     1981 aggaattata ggtgcaatgg atgaagaagt tagtatttta gttgatttaa tggatattag
     2041 agagactata aaaaaagcta gcttagaatt ttataaggga attttggaag gtaagaatgt
     2101 agtgttagta aagtgtggca taggtaaggt taactctgct ctttgtgccc agatactaat
     2161 aagcgaattt aaggttgatg ctattgttaa tacaggtgtt gcaggagctt taaacgaaaa
     2221 acttgatgtt aatgatatag taatatcaac tgatgctata cagtatgatg tagacactac
     2281 agcatttgga gaccctaagg gagttatacc aagaatgaag acttctgtat ttaaagcaga
     2341 tgaaagactt atagatgctg cttacaaatc ttctgtggaa gaagtaaaga ctcataaagt
     2401 attaaaagga agagtagtta caggagataa atttataaat tctaaagaat taaaagaaga
     2461 attagtaaat gattttggtg gatattgtgg agaaatggaa ggtggagcta tagctcatgt
     2521 atgctattta aataatactc catttgtaat aataagagca atgtctgata aggcagatgg
     2581 aagtgctgat gttacttatg atgtatttgt acatgatgct gccaataact ctaaagacat
     2641 agttttaaat atgttaaaat caatataaat atataaggag gtacaaaaaa atgagtgaaa
     2701 aaaaagttat attaagtggt gcacagccat caggcaaaat gactttagga aattatttag
     2761 gagctataaa aaactggact gaacttcaag acaattatga ttgttattat agtatagttg
     2821 atttgcatgc aataacagta cctcaagacc ctaaagtttt aagagccaat actatagaat
     2881 tacttgctca atatatagct tgtggattag acccagaaaa aaatactata tttatacaat
     2941 cacatgtcaa agagcatgtt gaacttatgt gggtattgaa tactatgact tatatgggtg
     3001 aattaagtag aatgactcaa ttcaaagata aatcacaaaa gagtgaagca aatctaaatg
     3061 caggtttatt cacatatcca gttcttatgg ctgctgacat attgttgtat caaactgacc
     3121 tagttccagt tggagatgac caaaaacaac atttagaatt agctcgtgat ttagcaaata
     3181 ggtttaataa tagattttca cctacttttg tagttccaga aggatactac ccaaaaggtg
     3241 gagcaagagt gatgagtttg caagagccaa ctaaaaaaat gagtaaatcc gattcaaatg
     3301 aaaatgcatt tatactatta gcagatgatt cagattctat aaagagaaag ataaaaagaa
     3361 gtgttactga ttctttaggt gttgtaaaat acaatgatga acaaccaggt attaaaaacc
     3421 taattgatat atattctaat ttaagtaaaa agagtgtaga agagattgta aacatgtatg
     3481 aaggaaaggg ttatggcata tttaaagaag atgtggctga agtagtatca gaagctttaa
     3541 gacctataag agaaaaatat gttgaccttc ttaataataa agattattta gaaaaaatat
     3601 atagtatggg agcagaaaaa gccgaaaaac aagctagaaa aactcttaga aaagtttata
     3661 aaaaagttgg atttatagag agaagatact aattattagc tatctttact tgataaataa
     3721 attttaaagt tattaaaaag gtatatgata tgaaagagag agtctcaaag tgaacttttg
     3781 ttcatgaggc actctttttt gtatgaaata aaatatattt taatcatttc aacaatgaaa
     3841 aagaggatta tctctatttt gagacagcat cttttgataa taaaaaaaga tgagcttttg
     3901 ctcatctcaa aggggttaga ttattcttac tttggggaaa gtagaataaa ggtaaaagtt
     3961 aactgatgtc aactttgtct tttttgcata tcactactga gcataaaaaa atcagaaaac
     4021 catagccttc tgaaacaact tctaacatac attgtatttt atacccaata tgtaatttat
     4081 gagttaattt taactcttat aatgaaaatt gtcaataata tatcaaaaaa atatttttta
     4141 aatttatata attaaatgaa tttatgttta tatataggtg atatgataca gataattatc
     4201 tttttaatac aatatagttc taaaaaagaa tatattagaa gaagataata tttaaataaa
     4261 aaatacctta taaagttaat tttttaatac tttataaggt aaatacatat tgtattgtgg
     4321 ggataaattt aaatcatttg atactaatca acactaagaa ccaataactc tctatcatct
     4381 tgagtaattg tatatattct taatttcttg tatgtcataa tattttctcc acctgtaaaa
     4441 taactcctaa gattaacaca atcagtttta ggttgatttg tatctcctaa ataattacgc
     4501 aattctttat ctttagtttc aatatcttta aatccaaaat attgtaaagt agcttcaact
     4561 ctatattttt ggacataatc gagagcttct ttattagttt atgttggtgt aaaatacata
     4621 ccatttatat aagcctttat tgtacttgga ttaaatttaa aatctgcaga atatatttta
     4681 tgtgtaacta ttatttcttg aacagttgga attttaactt ctggttcatc aagtatttca
     4741 ggagtactat ttagctctgt aatagataag ttatctaagt atattgtatc actatcaatt
     4801 aatgctatat ttatattaga agaatctttt tcagtagttt caaattcata actaaatttt
     4861 gtatatcctt gttctagtac caaataatcc gttttttctt cttttacttt tatatttaca
     4921 attattgaat tatatgttaa aggatttttt gaattttact aaaaacataa cgtttataag
     4981 gttttagttc agacataggt attttaatct tcgtctcacc atttaatcta tttgctgagc
     5041 cttttaaacc atcttgattt gtagtattaa cattactcca tatactaggg taattattgt
     5101 aatcatcaaa attagtaaag taagttggtg tttttataag tatattcatt cctctttcaa
     5161 gtttaacatt acatatttgt ttatcagaca aagtttttaa actgtcttta atcttattgc
     5221 ctatattatc atcaaatatg agttcaacac aactttcatc tattggtata ttattaaaat
     5281 ataacaaacc atctttttta gtagtgccaa aagttttttc aattgcttct ccaattgtaa
     5341 gttcaggtgt tttatcttct gggacctgta aacttttagc agtaattctt cttttgtaag
     5401 attcattttc tgtatctaat ataatagata cagaaatact gtcaatttga cctatatcgt
     5461 cttaccaact atttcattct gttactattt gtccagaacc attttttgta ccaaaatttc
     5521 cacttacttg tgttgtttct aatttaattt gctttccatc atctaatttt tttaattaat
     5581 tataatttat aggaatcagt ctagaactaa attgatacat tgtgttaaga gccataggtg
     5641 aaaatccttt tttgagataa gtatttccag gagatagatt attgccaatt tgattttctt
     5701 gtgctttgat agttgataat gcatctccat ctaacactaa atttgttgtt ggtgtcactt
     5761 tgtacatagg tgcagtacct gtgttgtagt atctaatatt tgcatttata tatgcagatt
     5821 ctcctttgtt tatacttaat ccagtatttc atgattatcc attgctattt tgaacagcag
     5881 ttgaattatc tgttgtatgg taataatttg tcttgtatag tttctttaaa ctcattaaat
     5941 ttttctaatg aaatttcgtt ttgatttttt gttcttatta ctttattaaa tgcaaatttt
     6001 tctggagatt caaaataata tacatacata ggtttatcta ttttattttt tgatatagca
     6061 tttcgaagtt ctctatattc tttttctcaa gaatttgctt ctatttgata atcataaaaa
     6121 taatttcttg tctgagaata tttgcttatc tctatagaat cttttttata tgatcctaat
     6181 acttcttttt cagatttttc aagtttttga tcttttcttt ctcattcttt agccttttct
     6241 ttatctttaa aaaaatcctt ctgatctttc tataggagcc ttgtaagtag tattacaaac
     6301 ttttccacta tagctttgta aatcttgtgc ataaatgtta ttaggtaaca aactacctaa
     6361 agttagatat aatatcaaca atatagatat acaattacta atacttttat gtttcctaaa
     6421 ttttttcatt tattctccct acaaatatta gtttagttaa cttttgaatt ttaatttatt
     6481 tagattatat aatctaaata agaatttttt atataaattt cataaaatat gtaggtatat
     6541 atcataaaaa tatgtattta tatgatatgt cagtacaaaa taacaactga atattttaaa
     6601 gagactattt cgtactttta tgatttaatg agctatctta tcgaatcata atctaggaac
     6661 aattgttata atatctaata gtagttatat atgatatagt cagtatgtgt tttttcacaa
     6721 tatgattatt atatagagga ttcttaggga ttactctttt tgtataaaaa agagatggtt
     6781 caaattttta tcttgagaca tctccttttt ctatttatta tgttttaata atattcttta
     6841 aaatatttct atatgtatta gatatacgac atatatggcc attactcatt ttaatattat
     6901 ttttctttag gtcgagagaa acaatatgat atttattaac tacaaaagat ttatgtacac
     6961 ggataaagca tgcatctaaa tctggtaaag actttaaact tttatagaat tcaaatattc
     7021 tagaagtagt cactaatttt attttatgtg gaatagtgct tgtttcaaaa tatataactt
     7081 catcaaatga aattttccat agtgagaaat ctgaatagat agtaaaaaaa ttagagcgat
     7141 aatttttttc tttaatgtat cttttgtgag caattttaat acaagctttt attttacttt
     7201 cagcaatttc catatcatgt tttagtataa agtccataac ttctaaccta taatcgaaga
     7261 tccctaaaat catttctata tgatttgtaa gaaatatgat tggactaata taatctgatt
     7321 ctctaatttt ttgtgctatc cataatccat cacataattc attacttatc ttatattttg
     7381 cgtctacaac tataaaatat attcttactt tatttttttc agtaagctct tttaataaca
     7441 aatttgcatt atgatataat cttacaacat cattatcaaa accttctttt attaaaatat
     7501 tatttatttt ttcttttagt ttcattccaa aacaaacatc attatcaaag attaatatat
     7561 ccactaaata ccctcctata aaaaattcaa aagtaaaaaa acaaaagtat tttatagaaa
     7621 atacataaaa taacactagg agataaataa gattaaggat agttagaatt aaggtatatt
     7681 tatgcttg
//
LOCUS       DAMJWU010000136         7677 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMJWU010000136 DAMJWU010000000
VERSION     DAMJWU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.141"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(489..1151)
                     /locus_tag="PC460_003319"
                     /pseudo
     CDS             complement(489..1151)
                     /locus_tag="PC460_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1311..1847)
                     /locus_tag="PC460_003320"
     CDS             complement(1311..1847)
                     /locus_tag="PC460_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2602175.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNGVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1920..2333)
                     /locus_tag="PC460_003321"
     CDS             complement(1920..2333)
                     /locus_tag="PC460_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2602176.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2799..3416
                     /locus_tag="PC460_003322"
     CDS             2799..3416
                     /locus_tag="PC460_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF2602177.1"
                     /translation="MDEKILELLQEMQGSISGIEGTISDMQSNINGIEGTISDMQSNI
                     NGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISSMDGTINS
                     MQDSITDMQVEIKEVNSKLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINR
                     NTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(4024..4203)
                     /locus_tag="PC460_003323"
     CDS             complement(4024..4203)
                     /locus_tag="PC460_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2602178.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNNYNIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4429..4962)
                     /locus_tag="PC460_003324"
     CDS             complement(4429..4962)
                     /locus_tag="PC460_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2602179.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPDFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGINRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(6383..6955)
                     /locus_tag="PC460_003325"
     CDS             complement(6383..6955)
                     /locus_tag="PC460_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2602180.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(7017..7280)
                     /locus_tag="PC460_003326"
     CDS             complement(7017..7280)
                     /locus_tag="PC460_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2602181.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7280..7501)
                     /locus_tag="PC460_003327"
     CDS             complement(7280..7501)
                     /locus_tag="PC460_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2602182.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2660 a         1325 c          939 g         2753 t
ORIGIN      
        1 acatatttaa gaaccgtaag tcttaacttt tatcattttt gaggttgtcg ttcacataag
       61 ttcgctacta cctatgcagt tctcttatga acttcttaca ctttcatgca agcacagact
      121 atatcttatc cttcggcatt atccgttaag gtctacccac ttccactacc aatagcttgt
      181 agtgtacttc cctcaagagg aatagtcgtt gaagtttctc ctattcggag tttacctgct
      241 gattgcccat tttaaagtac ttaggattta accttatacc atctaaccaa ttttttctac
      301 tttcgtaact ttcacactta ggtttatttc atccttatgt tttagtttgg ttagctttag
      361 gggtttccag caattcaagt agtattggat aggttctatc ctatctctac atataggttt
      421 ccctatatgc tgactatttt agctactaac tcatgactaa agtcacaagt gtgcgtagct
      481 attttttaat caaactggaa tacagactcc attcctagga aattggctga aatatagtct
      541 gctgtcaaag atgctgtcat cacagaaatt aaaacagata ctgaaaagtt tttatggact
      601 tcttcaagtg aaaacataac acccgccagt ggtgcatgaa aggctgctga taatccagca
      661 cttgccccac aggttaacag gaatttttcc tctgtcttcc ctcggtcaag agctctagaa
      721 atacctttac ctgccatagc accaagctga atggaaggac cttcccttcc caaagcaaga
      781 ccacccatga gtgataagaa tcctccaata aattttgttg gcaacactcg ccaccatact
      841 tgttttattt tccctgtcat ttccccctca agctgaggga taccacttcc agaaatcatt
      901 ggctcccagt ccaccaactt tgatacaatc catgcaagta ctgcaagtac tgcaaaccaa
      961 gctattattt ttattgtaga accttctaca aattttaaaa tttggctaag ccaagtacca
     1021 gcctgcccta aaagtatcct gtataaaagc acaatcaaac cagccacagc cccaaccata
     1081 agtccttcac caatcaaaat tacctgaaag tgctctgccc gtttgataat atgagatgta
     1141 tcctttttca tactgttcct ccttcatcca ataattctac acaatctgaa tctccaatga
     1201 ctaaagtcat gagtgtgtac agatattttt taatcaacaa aaatagattt ttatttacta
     1261 aatataagaa aataattgct caatttctca ataaatgtaa atttattctt ttaggtattt
     1321 attctccaca gtcgtgcttc ttccagcaaa atatttatta gttcttgctt tctctgtgta
     1381 tagattttac gattgtcctc aaactgtaat gccaattttt gtttatgact atcatagagt
     1441 tttgctttgt ctgtaaaagt atttagataa tctcgaaaat tgatataatt attccatgca
     1501 acaccattcc attcaacaac atgaatatgg tgagtacgag tatttttctc aaaatctccc
     1561 atagcaaaaa gtaattgttt tggtacatcc tgacctcgaa aaacgattcc acattgctct
     1621 aacctttcga ggtataattt agcttcctct aaagaagcaa cacctacaac aatatcaata
     1681 ataggctttg cgctaatact gggaatagaa gtgcttccaa tgtgttgaat atcaatggca
     1741 gtcttatccc atatactttt caataatata atgacatctt ttgcactttt atcccatatc
     1801 atctgatgtt ccaataattt aaccatacca cattttaaac ctatcactat ataccacctc
     1861 aatataatcc acctttatat atattacaat tttttctctc ttgataaaca gtgatttagc
     1921 tatttcactt tttcttgaca ctcgctacat tccctatcat gaatagatat aattcctcca
     1981 catttagaac aagtatatct ctccttctgc ttttgcataa acttctctaa accgtactgc
     2041 tgaacaaatt tactattttc cataaggctt gcttgatatc tcttgttata acttttttca
     2101 agatttttta tgagtttaca aggaaaattg aaacattcaa aacaataaaa caaccctttt
     2161 tgacagacac aatcctttat tttacactta cggcagtgtt ctggctttcc tgtatcgcca
     2221 ttcaaacaac cagcacatgg ctttttgtga taacaatgct tatagcaaac catacaattc
     2281 attccacaag gagcaaacat aatcgtatca atactctctt ttggcatttt cataacattt
     2341 tactcctttc aaaaatccaa tttgtcgaat tgttatatat tcatattgta acataaaaaa
     2401 tagctaaaaa attcatgcac tttcattgcc aataaatttt caaggaaata tgaaagttta
     2461 aaactaagaa taaaaagaga taaaaaggaa gctactaatc aaattattca aatacaagta
     2521 atcaatataa ataacttaag gtatatttaa aagtatatga tattaccagc agttttaaat
     2581 acaaatataa ccacacatgc ctcaaaaaca cttattacag tatcttagaa acttagacaa
     2641 tacataagat tcattaataa aaaagttctc aatatagata tatttaacca tgttttgaat
     2701 agcagataat catatgatta aaaattaaat atgacatagc gtttgtggaa tataacacct
     2761 aaattatgtt ataataataa aacaggagtg tgatagtgat ggatgaaaaa atattagagt
     2821 tattacagga aatgcaaggt agtatcagtg gtatcgaagg cactattagc gatatgcaaa
     2881 gcaatatcaa tggtatcgaa ggcactatta gcgatatgca aagcaatatc aatggtatcg
     2941 aaggcactat tagtagtatg caaggcaata tcaatggtat cgaaaacact attagtggta
     3001 tgcaaggcac tattggcgat atgcaaggta gtatcagtgg tatgaatggc actattagcg
     3061 atatgcaagg cagtatcagt agtatggatg gcactattaa tagtatgcaa gacagtatta
     3121 ctgatatgca agttgaaatt aaagaagtca atagtaagtt agacaatatg gaacctgata
     3181 ttaaagactt aaaaactgga caagttgaaa ctagtagtag acttgatact attcagagtg
     3241 atgttacaga aattaagtca gatgtaaaaa ttaacagaaa tacattagat acagtgatta
     3301 aacatacctc ttcattagta aaagatacat cagatattaa aaatgattta aataatatag
     3361 agatagtcac atctaagaat tcgaatgata ttgctaaatt aaaatcagtt aaataatata
     3421 ccatagtaaa aaatacctga aaatttcaca tacttcttta aagttatacc aaattttata
     3481 cagaaatact gattatccga caacacactt tacttataaa taatttatta aaaaaatact
     3541 cattttatcc taaaattaac acgactaaat gagtcttgat ataataataa aaaataaggg
     3601 agattggaca ctaaaaagtt gtgtatctcc cttatatcct ataatatagt tatataattt
     3661 ttcagcctaa aataaaagtc catctttgat taaaacacat ccccttatca cttataaaag
     3721 gataaaatct tgttttcatt tttccattta tatcttcttg cttaagagta ataatttttt
     3781 cttttgatac tttaagtgtt tggttaaatt taactaatcg actaagtttt gttcaacatc
     3841 tatttttcat attttccaat acttaatcta ttttttataa ataattcttt catttctaac
     3901 tcacttacat tttttacata aaacgaaaca tataaatttc aaccttatct tgtatggttc
     3961 ttaatactta atgagtagct taagtatcaa tcattataaa tttttccctt acatttaaca
     4021 ctcttaccat ttccaaaata tacaaaatat attttgaaca tttgtacaat gatgaaatac
     4081 aatacatgaa aatacaatat tataattgtt gtctaaaaat agaagttctt caacataacc
     4141 tttcacaaaa aacatattat ctaagtattt tttgtctgct ccttgttttc ctgtttgtag
     4201 catataaatt acttcctcta aacatataac tactcacatg aaaaaagaaa ataaacttcc
     4261 aaaatacaag ctctaaaaac tacctctaaa gttgtataat gtttactagg aataaggttg
     4321 tataacatat agtttaaata atcatttttt aaaattgcaa gttattgctt tcaaaattag
     4381 atgtttgtat atcaaataaa tgattaagaa tttctaaatt taatggcttt ataatgcact
     4441 ccctttcttt gggataaaat aatgttgcac agatactttt tctaaagata acaattttat
     4501 ttttctatta attccaccag catacccagt caaactccca tttgttccta caacacgatg
     4561 acatggaata ataatagaga ttccattatg ccctactgca ccaccaattg cttgtgctga
     4621 cattttagat attccttttt gttgagcaac tttatatgca atttctccat aagtgattgt
     4681 tttaccatat ggtatttgtt gtaaaatttt ccacactgta gtctgaaaag gagtacctac
     4741 catatggagt gatggtgtaa aatcgggttc ttttcctgaa aaataaatat ctaaccattt
     4801 ctttgtctgc tcgaaaattg gcaattcttt ttcttgatgt tcttggtcaa gattataagc
     4861 ataatacttt tgattctcaa accaaagacc agttaaccca atattatctg atgctaaaag
     4921 aatatttcca attggactat ggtaatagct aatatattgc atacaattcc tccttatatt
     4981 ctgaaaactt cactcaaatc attagacact tcttttctat ctaccattat gtaaaaacat
     5041 actaatataa tttccttcta actacacttt cgttataata tctatcctca tatcatatta
     5101 gttttatatt tacctgcatc tgttcatcct tttttccctt tccctttgat ggatataaaa
     5161 ttcatacatt taagcttcct actgtattaa cttttataga agtgtatctt ataattttat
     5221 cactaaatag accaaaaaaa tatcgatata gcatcaagta atatcataat actttcaaat
     5281 gggaaaagta agaataggct gtaaaaaaga aaagtatttt tatgttagaa atccagtaaa
     5341 atcgttagta gatagaataa cagaaaacga acaatttgct atttatcatt ttacacataa
     5401 atataattga cgcaataaag tgttttctat tctacaacga ttaataaggt tagaaaaaag
     5461 taaaaatgaa ctgtatatgt atgaagcatt agtttctgtt tctttccttt ggcttattat
     5521 gataaaaaat attgttttgc caccaataaa acacaaaaat ataattaata tccatatgca
     5581 aaaaatttta cgttatattg aaaaatacta ttgttttaca ttatattgaa gggtactatt
     5641 gtgaaaactt aattttggag tactatcaag cagtggtaat atcaataaat cagagtgctc
     5701 cagatgattt gaactaagtt taaatactat accttataaa tattttactg agttttgttt
     5761 attaaaagca actcaatttc taaaaggcac aaatgagcta ataagtgaca ttactataaa
     5821 agttgttttt catcaaataa tctattttag taaatgtttt aaagaaaaaa cgggatattc
     5881 tccaaaagtg tacagaaata taaaaaaaga gatatgattt tttatttctt ttgattatcc
     5941 aaagagtaaa taaaaaatat tgtaaaaaac ttactaacaa cggcaatgct ttgaactatg
     6001 gtaatacttt gaacttttga ttggactttt tttgctaaaa taatatattt aaatagcaaa
     6061 gtaatctgtt taatcaataa aaaataagag ataaaagaaa tcaactaaaa cttttacctc
     6121 ttattttgat agtttatcag agctaatatt tacactcttt ttatttttga tacttcatta
     6181 aaaatgatgc ttattctctt ttcattttta atacttcatt agaacttatg cttattctct
     6241 tttcactttt aattcttcat tagaacttat gcttactctc cattcacttt taattcttca
     6301 ttaaaaatga tgcttactct ccattcactt ttaattcttc attaaaaatg atgcttattc
     6361 tccattcact ttcaattctt cattaaaact gatgcttgca ctctgtaaac tcataaacat
     6421 ccattaatgc ttcaccaaat ctttgatagt gaactacttc cctctcaccc aaaaatctca
     6481 atatatcttt caaatcaggg tcatcagtta aatttattaa ttgatagtac gtagccaatg
     6541 ctttttgttc agcagccata tcctcatgta aatcagttac cgaatcgcca aagacattaa
     6601 tataacttgt agtaaagttt actccattag aatctactgg ctgaagacca tgtccaaaat
     6661 tagcataatt actaccaagt ccagcagcct taagctcttc tggagtacaa ttgcctatta
     6721 attgatataa cactgaagaa attatctcaa catgagccat ttcctctgta cctatatcag
     6781 ttaaaagtgc acgagatttt ccagttggca tagtatatct ttgttgaaga tatctagttg
     6841 cagctgccaa ctcaccatta ggtcctccca actgagtcat gatatatttt gccattctag
     6901 ggtctgcttg tcttatgtta actggatgtt ccagtgtttt ttgataaatc cacatattat
     6961 cttactcctc cttaatatat atttaataat aaatctaatt ttaaagttta ataattttat
     7021 ttataaaatg atttgtccca tggccaaggt tcagatatcc atgaccaagg atattcactt
     7081 gattcatatc caaaattagt taatgctcca tatttagatt cataatcaca cacagcttgt
     7141 gtaaatttat cgcagtatct gttgtatgaa tcaagagctt tcttatccct tggattgtta
     7201 tctaaatata aatttaattc tatacaggca aattgatatt ctgaaattct ctctaaaagt
     7261 tctcttctat ttaatgacat tagtacatgt tacccccttt tttagctcta actctatttt
     7321 taggatttgc atatagccaa tcattatagt tatttgactc tattaaatct aattcaggaa
     7381 ataaagtacc tctttttaat gctgtttttg gattgtatgt gtcatcataa atttgacgat
     7441 tgatgtatgg tctagcaaat tcacaacaat agtcttgttt atcctctttt aaatatccca
     7501 ttaattatcc tccttaattt ttagaatttt aaaatagcat tatttttaaa aattgtatac
     7561 tggttctata ttataactgg tagttttaat aggtctttaa taagattaaa tgattaaata
     7621 agtatctttt tttcttattt tacctctaaa atttttctaa ttaatctttc ataaata
//
LOCUS       DAMJWU010000137         7484 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMJWU010000137 DAMJWU010000000
VERSION     DAMJWU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.142"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(72..770)
                     /gene="yunB"
                     /locus_tag="PC460_003328"
     CDS             complement(72..770)
                     /gene="yunB"
                     /locus_tag="PC460_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF2602183.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            984..3677
                     /locus_tag="PC460_003329"
     CDS             984..3677
                     /locus_tag="PC460_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2602184.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFKENNEWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            complement(3951..4886)
                     /locus_tag="PC460_003330"
     CDS             complement(3951..4886)
                     /locus_tag="PC460_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF2602185.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(4930..6102)
                     /locus_tag="PC460_003331"
     CDS             complement(4930..6102)
                     /locus_tag="PC460_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2602186.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            complement(6089..6553)
                     /locus_tag="PC460_003332"
     CDS             complement(6089..6553)
                     /locus_tag="PC460_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602187.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(6568..7410)
                     /locus_tag="PC460_003333"
     CDS             complement(6568..7410)
                     /locus_tag="PC460_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2602188.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
BASE COUNT         2538 a         1300 c         1058 g         2588 t
ORIGIN      
        1 ttaatattat ttccaaaaat tttatttata aactattatt ttgaatttat ttaaaaatat
       61 acaactttat attacttgtt ggtattcttt ccagtgttag gtaaaacatt tcctagattt
      121 ttttctggaa tatttacata actttctgga acatcaccta ctattatagt ttcacaaata
      181 ggtatttgag ctttaatttg ttttgttgaa gttataagtg gtattactac tttaacttgt
      241 gtttgagctt ctaaatatat tctatgtcta gtttgattta tacctgaaga ctcaaattcc
      301 gttttaaagt ttactgatac tgttcctata ggttcaatag atacttcaag tttaggaccg
      361 tatttagcta atataggact tcctaatgct gttcctatag gtatgtaaga acttgctgtc
      421 ttatcctttt ttaattcatc ttgtatttct aaagctattg cagatgcaat ttcattcatc
      481 attatagtgt ttgcttgtat catagttatt ttaccattat tatcaagttg tatatccatt
      541 aaatcttcat aatttatttt tccttctacc atctcagcaa cagctttatt tatcgaccta
      601 tttgctagtt caagagcttt agtctctgct aaaactgtta ttgtcggtct taaagtttta
      661 tctatatata taaaactgcc tataaataca gacaagaaaa gaattactaa aaaaatagct
      721 atgatttttc taaattcatt ttttgttttt ctatctacta ttctattcaa aattatcccc
      781 tcctatttat ttatatgtag ctttggcata atttttataa tatttgtcaa aaataaataa
      841 aataaaaata tagacttttt aatatattac gtttatacat atacaatgta cagaatatta
      901 tagcaaattc attttttcta ttaattacaa aactactcct atttgtgtta taatattgca
      961 ttaagtaagg aggttaaaat cttatgaata acgataatga taaaaatgga aataaaatca
     1021 ggagaaaaaa agttagctca tctggtaata caaataaacc aattaataga acttcagcta
     1081 acaagactcg ttcaacaaaa aacaagaaaa aatctaaaaa aagcgataaa tttaaaaaac
     1141 taagagtatt tgggatagta tttttagttt tactagttgt tggtacagca ggtactgctg
     1201 gtctagtatt tgcgtcacta agagatgtaa ctccagttac agaagctgtt ttagataaac
     1261 agacgaacca aacaactact attaaatacg ctaatggaaa aacattatct acagctccaa
     1321 gtgtaaataa aaagacaccc gttccattag ataagatttc cccttatctt caacatgctg
     1381 taattgcaat agaagatgag cgtttttatg agcataaggg tgtcgatatt aaaggtttat
     1441 ttaggtcagt attaaagacc cttacaggta ctaagcaagg tggtagtact attcctatgc
     1501 aggtatccaa aatgctgtta acaactgaac agcaaactat acctcgtaaa ataaaggata
     1561 tttactatgc acatgagatg agtaaaacag taagtaaaga taaaatacta gaaacttatt
     1621 taaataactt cttcgttggt agaggtctag caggtgctga ggctggtgca cgtggttatt
     1681 ttgataagtc tgcagctgac cttactctag cagagtcagc acttcttgct ggttcaacta
     1741 aaaatccatc aagattttca gcctataaaa cttctaagtt agagggaaat gaaactaagg
     1801 aggatttaga gaataaatta ttattttttg ttaacacaac tgatgatgat ttagatgacc
     1861 ctactcaagt ggactttgat atgattgaaa aaataaaatc ttgggagctt atatctaatg
     1921 atacttatag acaattaaaa gcaggtacat tagttgttag aaaggctgta agtaatcctg
     1981 aagctaaaaa aagacaagaa attgttttaa agaaaatgtt agaattgaaa tatataaaac
     2041 aaagtgaata tgatgaagca atcaaagcta aaatagaaat aaaattgcct caatcatcag
     2101 ataaagtttc ttcatctgta gaagacctta tcgaaagtga agttataaat gctttaatgg
     2161 aacaaggaca tacaaatgat gaagctcaaa acttattcta caatggtggt ctaattgtaa
     2221 acacaactat agaccctaaa atgcaagatg ctttagaaga agaatttgat agaaatagca
     2281 atttcccagg acatatggtc ggtcctgatg gagttagcca accgcaagct gcaatggtaa
     2341 tcttagatta taaaaatggt gaaataagag cactagctgg aggaagaaat ataagtggta
     2401 gaaaaacttt aaatcgtgct actaaccctc atcagccagg ttcttctata aaacctttag
     2461 caatatatac gccagctatt gatactctaa aaataacaca agcaactgct ctaagtgatt
     2521 ctcgaggagg atataagttt aaagaaaata atgaatggaa tccaagaact actactgctg
     2581 gtcatggttc tatgagttta cgtaaagcac ttgctaaatc ttcaaacacg atagctgtca
     2641 aaactgctga aatgcttgga gactcttatg atgaatgtgt agatataatg atggattact
     2701 taaagaactt tggtataaca actttaaaga ataatcactc aggttcatct gaagcaagtg
     2761 atagaaaatt cccatcattg acactaggtg gtatggcgaa tggtattact ccacttcaaa
     2821 tggctgctgc ttatggtaca ttggcaaatc acggtattta tgtagaacct tctattttta
     2881 ctactataac tacctttgat ggtcaattat tagttaaaaa tgctcctgaa gaacataaag
     2941 ttgtagaccc tgaagtagct tatgttgtta cagatatgtt agaaagcgta attactgaag
     3001 gtactggtgg tgttgctaca cttccaaagg gaatgcctgt agcaggtaaa acaggtacta
     3061 caaatagtgc atatgatgct tggtttgtcg gttacactcc ttattatgtt ggtgcaactt
     3121 atattggaga tgatgctggt agaaaagatg attctggtaa cactataaaa cgtagagaag
     3181 tcccacatgg aagtactagt actgcaaaac tatgggaaaa aattatggaa aaaatacatg
     3241 ctaatttaac ggttactgaa tttgaagttc ctaaaaatgt atattttaca aaaattaatt
     3301 tagaggatgg aggaaagcaa tcttctggct ctaaagcagc atttattgaa ggtactgctc
     3361 ctactaaagt aagttctcaa ccatcatctg aagatacaaa aaaaccagac aataaccaac
     3421 cagaagaaaa taataataac aataataaca caggtgataa tggtggcggt tctacacctc
     3481 ctgataatgg cggaaacaat ggtggtggct ctactactcc tcctgataat ggcggaaata
     3541 atggtggtgg ctctactact cctcctgata atggcggaaa caatggtggt ggctctacta
     3601 ctcctcctga taatggtgga aataatggtg gtggctctac tactcctcct gatggtggtg
     3661 gaactccagc aacttaataa aaaatagaat ttaaataaaa atataaggag ttgtctcaag
     3721 gtgattaaaa aaatcacttt gaaacgctcc tttttttata attacaatat attatttgta
     3781 ttcataaaaa agagaatccc aataaccaga atcctccttt ttatataaac aaataaatat
     3841 tttattttta aagtttttaa ctaagtgctt tattcttact tttattccta attgtttata
     3901 tattcttaaa tagttatatc acaaattata taaacttaat atttttctaa ttactgtata
     3961 ccaaatgttt gaaatctatt taatttgcca tatctcttaa taagcatttc ccactcttct
     4021 ttatcataga aatcacactt atttctacca aatagcttgg caacttcaat tgcaaatctc
     4081 ccagctgaat ctaaatcact aaaattagtt gcacctgtag cacatccagc aacaggcatc
     4141 tctgttgtaa ttgctactcc tactattggt gcatctgtag ctactgctgg ttgtaaaata
     4201 ctatttaagt gatacagatt atttccatat ggtgttatat cttgaataga caaagggaat
     4261 acaaatggag atttccctgt tacagtctgc ataatatcta aaaggttatc actagttttt
     4321 aatatgtagc cttctttaac agttggcgat attgcaaatc ctctagtatt gattactcta
     4381 tttcctttag tagtatctat agaaagtatt gcatcacaac tactatcaac ttcattttca
     4441 ttcatagtca tcatatcaat tggagaatcc ataaaaggag taggatagtg ttctttagtt
     4501 ggagcatctg ggcaaatatg ggttgaaatt acaacatcac catctaatat atcccctttt
     4561 cgttgcatat ccaaaagttt agcagccact gcaattgcta ccaatgctcc atcaccatct
     4621 gaaacaaaac ctataacttc tggtcttgct ccaatccctc ctagtctacc taaaatgcca
     4681 agagtaggag catctccacc ttttaatttt ccatttttcc caggaattgt aagctttact
     4741 aaatctgttg agcctttact tgaagacaat tcttttacag ttacatctac ttcaccataa
     4801 ccttgtaagt aagtttttac ttcttcccca ttagcatttg ctttatctaa aatgtcataa
     4861 acctctatta cttgttttaa tatcatgtct aatccgattt cctttcttaa tatattttaa
     4921 aattatatat tatttactat acaaaatatc tactccacac attgctaaca ccttaattgc
     4981 ttttataagt gctactctgc cttcttcacc accagctgca acagcaaatt catctgtatg
     5041 agttgcagta ttttctttta attttatata tgcttgtatt gcaggtatct catgagttaa
     5101 atttcctgta tcagttgaac ctataccttg acttaaatct ctttcaacat agtcttcacc
     5161 caaaacttca aaattttgtc taactatatt cactaattca gaatcattct taatctcttt
     5221 gtatatttca tctaattgaa ctatttctac attagttcca gtagaaattg cagctccttt
     5281 agcacaattt ttaagcattt cacaaatctc atttaaatat tctatgctta aagctcttac
     5341 attaaatata gctgtagatt tttcaggaat tatattatgt atgctacctc catcagttat
     5401 aattccatgt actcttgcat aatctttaag atgtaatctc atgctattta cactgttaaa
     5461 tagtgctatt actccactta aggcactttt cccttcccaa ggaaaagcag cagcatgtga
     5521 tgccttacca gtaaaatcaa attttaaatt aacttgagca aatgatatat catcaggcat
     5581 tgaagcatca cttggatgta aaattaatgc agcatctaca ttattaaata caccttcttt
     5641 aatcatagtt atttttccac ctactctttc ttctgcaggt gttccaaaaa cctttacagt
     5701 cccttctata ttatgttttt tcatgatttc ttttaatatg atacctgcac caacagaact
     5761 tgtccctatg atattgtgcc cacatgcatg tcccattcct ggaagtgcat catattcagc
     5821 taaaaaagca actgttttcc cattagttcc atttgaatac acagcttcaa aagatgtttc
     5881 taatcctcct acacctttag ttacattaaa ttcattttct tctaacaaat cacataatgc
     5941 tttttgtgac ttatactcct caaatgctaa ttctggattt gaaaataaat agtcagaaac
     6001 tttttcaaat gatgtctttt tctcttcaga taataacttt atttcctctt taatttttgc
     6061 ttttaattca ttcatattta ctacctcctt aaagcattcc atttaatctt aaaactgttt
     6121 gagatataaa agcagaacca aagtaagtag atgtcataac tataaatgat ataactacta
     6181 acttccaact caaagtcttt aatatatcta attggttacc tacagagatt cctgcaaaag
     6241 ctaataatgg tgttactgta gacataaaat tgattttccc tacatttgtt gctatgatat
     6301 cccccactgg agatattgga agcgataaaa caaatgcaac taatgtagcc catgcaaaac
     6361 ctggtaaatt aggtttttta acaaaattct ttattataat agcaattatc accaacaaaa
     6421 atccagatac acttccaatc aacaaatctt gagcctgagt cttaagtgct atcatttgtc
     6481 caaagcaaat aataatatat ataaacaata ctatttttat ctgctccata atagtcttaa
     6541 aagttttttc catatatata tcctccttta caatttatta cttgttcttc ttgtttttct
     6601 tcctctaaat ctttcaataa ctggagctac tttgctataa tagtagttgg ctactggtaa
     6661 agataagaat aattctatat atattccagt aacagctgta atcaatccac tagtagctga
     6721 atatgctaaa atctgctctg acatttgtgg aaccgtattc attaatgatg ctgttgcagc
     6781 tcccatcatt gaaccactac ccattcctga tgccattgct aaagcatatg gatgaaatcc
     6841 taaaagtaca cccactggtg ctaaaaatga aaataataat gttccaaata tactaccact
     6901 tatgtaagtt cctagtgttc ctataccttc tggtgagttt aatccatact tatctccaat
     6961 aattcctagt gctgtgtcac gacataatga aaaagtacca cctaatgcac ttcttccaag
     7021 acctaatgct actccaacag gtaatgctat caacattgta cccatatgac caaattcttg
     7081 taataaaagt gctggtcctg cttgaagtaa tataccaact ttaggccctg cattaattcc
     7141 aaacattatc cctaatggat atagtgtcaa agataataaa tatggagata catttatttc
     7201 attatcccct ataaactttt ttaaggctgg tataactctc cctaataaat gaggagaaac
     7261 agccattcct atgaatatag cccatagtat actaagtaaa gttacattaa ttcccataac
     7321 tttaaatgaa ataggtccaa ttaattctgc caatattgtg attataaagg ctgctattat
     7381 ggcatttcta atccctgctt tttctttcat ttctatacct ccttgtaatt tttataattt
     7441 tcttataata taattgttag tgattttatt atataaccta tcat
//
LOCUS       DAMJWU010000138         7456 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMJWU010000138 DAMJWU010000000
VERSION     DAMJWU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.143"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            86..1063
                     /locus_tag="PC460_003334"
     CDS             86..1063
                     /locus_tag="PC460_003334"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF2602189.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1056..2024
                     /locus_tag="PC460_003335"
     CDS             1056..2024
                     /locus_tag="PC460_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF2602190.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2208..2798
                     /locus_tag="PC460_003336"
     CDS             2208..2798
                     /locus_tag="PC460_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2602191.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2817..3587
                     /locus_tag="PC460_003337"
     CDS             2817..3587
                     /locus_tag="PC460_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF2602192.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3724..4692
                     /gene="dusB"
                     /locus_tag="PC460_003338"
     CDS             3724..4692
                     /gene="dusB"
                     /locus_tag="PC460_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF2602193.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4801..5280
                     /gene="greA"
                     /locus_tag="PC460_003339"
     CDS             4801..5280
                     /gene="greA"
                     /locus_tag="PC460_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF2602194.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5295..6824
                     /gene="lysS"
                     /locus_tag="PC460_003340"
     CDS             5295..6824
                     /gene="lysS"
                     /locus_tag="PC460_003340"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF2602195.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7133..7327
                     /locus_tag="PC460_003341"
     CDS             7133..7327
                     /locus_tag="PC460_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602196.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2977 a          751 c         1507 g         2221 t
ORIGIN      
        1 aatagtatta aaagtatatt aataatgtat aattatttat tagaagaaaa taagtcatga
       61 aattatgtca tattagaggt gaaatatgag agataaaata atagagatta tattaaataa
      121 taaatctgaa tttatttcag gagaagaatt atcaaaacaa ttaggggtgt cacgtgcagc
      181 tatatggaaa catatgaaag ccttaaaaga agagggatat aatatagaat ctgtaaataa
      241 aaaagggtat cgattagcgg aaaatccaac tgatttatta agcccacaga atatttacta
      301 taaattagat acagagttta ttggaaaaaa tattgaacat cttgacacta tagattcaac
      361 aaatgaacat gctaaaagga tagctataaa tagtgtagat gggactgtga ttttaagtga
      421 acatcaagat atggggaaag gacgattggg aagaagatgg gattcaaaac ctcatgaagg
      481 aatctggatg agtattatat taaaaccaga tatggaacca ttcaaagccc catttataac
      541 tttaatagca ggagcaagtg taacgaaagc tttaaacaat ctaggagcaa atacattaat
      601 aaaatggcca aatgatgtta ttttaaatgg aaaaaaaata tgtggtatat taacagagtt
      661 atctgctgaa attgaaagaa caaattatgt tgttcttgga attggaataa atgttaaaat
      721 aatggatttt gaacaagaaa tagctgacaa agccacatca ttgtataaag aagggtatat
      781 gttatccaga gtagatattg ttaggaatgt attgactgaa tttgaaaaat tatatttaga
      841 ttatactgta aataattcca aagaaaaaac cttaaaaata tgtagagatt attctgcaat
      901 tatagataaa aatatatatg tattaaaagg agataataga gaactagtga aatgtttaga
      961 cataaatgaa gagggaaatt tattagttca aagaagtgat ggaagtataa aagaaattat
     1021 ttcaggtgaa gtttctataa gaggagaaaa aggatatgta taataatatt ttggatatag
     1081 aaggcttaaa ggttgggcag gttgaggata gaaatggtct tactgggtgc acagtagtaa
     1141 tatgtgaaga aggagctact tgcggtgttg atgtaagggg ctctgctcca ggtactagag
     1201 aaaccgattt attagaccct gtaaatatgg ttcaaaaagt tcatgcagta gttttggctg
     1261 gaggttctgc atatggtctt gaatccactt gtggtgtaag tagatactta gaagaaaaag
     1321 gaattggatt tgatgtaggt gtaggtaaag tgcctatagt tgtaggagca gttttatttg
     1381 atttaggagt agggagcttt aaatgtagac cagatttaaa gatgggttat agagcttgtg
     1441 aagttgcaaa tactgatatt ttaaatcaag gaaattatgg agcaggttgt ggagccactg
     1501 ttggaaagat aagaggtcag gaatatgcta tgaaaggcgg aataggtagc tattctataa
     1561 aattagataa tggtctaatt gtatctgcat taattgctgt aaatgctctt ggagatgttt
     1621 atgaaaatgg taaggttata gctggtgctt taaatgataa aaagacagaa acaattgata
     1681 catataaatt aatgaaacaa ggtgaaagta aaggtggatt tagtgtagat aatactacaa
     1741 ttgggataat tgcaacaaat gctaagttaa ccaaagctgg atgtaaaaag atatctcaaa
     1801 tgggacatga tggatatgct aagtctattt ttcctatcca tacacctcat gatggagata
     1861 caatattcac aatatcaaca ggagaaatag aagcagatat aacattagta ggttcattgg
     1921 ctgtagaggt tgtggaaaaa agtataataa atgcagtaaa aaaagcggat agtgtagaaa
     1981 atataatggc atataaagat atagtaaaaa aacgagaaat atgatgaaat tagcatattt
     2041 ttcttgtcaa attgttgaca atggcaactc atcttgataa aattattctt gttagagcgg
     2101 gcatcctaca atggtagctt gactcatagt tataagaaaa aaacgaaaaa aattaaaaaa
     2161 ttaagtagtc cggcatctta atgagctgga acggaggtgt attttttatg atgaacacaa
     2221 gtgcatcagt aagaaaagta aatgtaagaa agatgactat aataggagta ctatcagcta
     2281 tttctattat gctatctatg actccattgg gatttattcc agttggacct acaaaagcaa
     2341 ctataatgca cataccagtt attattggag ctattatgga aggaccaata gttggggcag
     2401 caataggatt tatatttggg atatcaagtc tcttaaatgc tataacaaac ccaacagtaa
     2461 catcttttgt ttttataaat ccattagttt caatattacc aagagttatg ataggtgtat
     2521 tagcttatta tgtatatcag ttaataatta aggctacaaa taaggtatac atatcaggtt
     2581 taattacagg ggcaataggt tctctgttaa atacagctgg agttttagga atgatatatg
     2641 tcttatatgc tgacaagtat cttcatgcaa tgggtaaaag tggttcagca ggaaaagtaa
     2701 taatggctct tgccgcagca aatggggtgc cagaagcaat agtaggggct ttagtagtag
     2761 ctgctgttgc cactgtactt aagaaaagca aaaaatagaa agaaggttaa tgacatatgc
     2821 ttctagtatt tgatgttgga aatactaata tggttttagg tatatataaa ggtgacaaat
     2881 tagttaatta ctggagaatt aaaacagata gagaaaaaac gtctgatgaa tatggaatcc
     2941 taataagtaa cctatttgat tatgataatg tgaatataag tgatattgat gatgttataa
     3001 tatcatctgt agttccgaat gttatgcatt ctcttgaaaa cttttgtata aagtactgta
     3061 aaaaacagcc attaatagta ggtccaggca taaaaacagg tctaaatata aaatatgata
     3121 atccaaaaca agttggggca gatagaatag ttaatgctgt agcagggata gaaaagtatg
     3181 gagcaccaag tatacttgtt gattttggaa cagcaactac attttgtgct atctctgaaa
     3241 aaggtgaata tttgggtgga acaatagcac caggaataaa aatatctagt gaggcgttat
     3301 ttcaaagtgc gtctaaatta cctagagtag aattagctaa gccaggtatg actatttgta
     3361 agagtactgt atcagccatg caatctggaa taatttatgg atatgttggt ttagttgaca
     3421 aaataataag tatgatgaag aaagaattga attgtgatga tgttaaggtt atagctacag
     3481 gtgggttagc taaactgatt gcttcagaga cgaaaagtat agattatgta gatggttttt
     3541 taacactaga aggattgaga ataatatatg aaaaaaacca agaataatta atatacaggg
     3601 aattgggtta tatatccaat tcccttttta tgatagattt ctagctatat gtggtatatt
     3661 ataatgagga aataagaatt taaagttaaa tcacaaaata aaggcaaaaa taggagaaaa
     3721 acaatgaaaa taggaaattt agaattgaaa aataaagttt ttctatcacc tatggcaggt
     3781 gtaacagacc ttccatttag attaatttgt aaagaacaag gatgtgggct tttatataca
     3841 gaaatgataa atggtaaggc actttgctat gatgatgaaa atactaaaaa aatgcttaag
     3901 atagaagagg aagaacatcc tgtagcagtt cagatattcg gttcagaacc agagtttatg
     3961 ggaagagcag cagagataat gaatgattat tcaaatgaga ttctcgatat aaatatgggg
     4021 tgtccagctc caaaagtagt taaaaatgga gatggttcag cacttatgaa aaatcctaag
     4081 cttgcagaag aggtattgag ggcagtagtg aagaactcaa aaaagccagt gactttaaag
     4141 attcgaaaag gctgggatga taatagtgta aatgctgttg aaatagcaaa gatagcagag
     4201 gattgtggaa taagtgcttt agcaatacat ggaagaacta gagaacagtt ttatacaggc
     4261 aaagcagact gggatataat tgcagagatt aagaaaaatt tgagtatacc tgttatagga
     4321 aatggtgatg tatttacaat agaagattca ataaatatgt tagacaaaac tggttgtgat
     4381 gctataatga taggtagggg tgctcaaggt aacccttgga tatttaaaag aataaaccat
     4441 tatatgaata caggcgagat tttgccagag ccaactctta acgaaaaaat aagtactgcc
     4501 attaagcatt taaaacttgc agtagaagaa catggagagt atgtagctgt aagggaaatg
     4561 agaaaacaca tcgcttggta tttaaaagga cttagaaact ctgcaaaatt aagggatgaa
     4621 attaacaaaa tagaagacta tcaagaagtt gtgtcgaaat tggagtacta tatggaagac
     4681 tccttgacat aaaaataata gtgacttata atatagcgca taaattaaaa aaagattata
     4741 aaatttttaa aatataatta ggaaaattcc taagaaattt gtgaaggagt tgtatttata
     4801 atggaagaaa ataaagagtt tttattgaca caagagggat atgataaatt agaagaagaa
     4861 ttagaaaact taaaggttgt aaagagaaaa gaagttgcag agagaataaa agttgctata
     4921 tcttttggtg atttatctga aaatgctgag tatgatgaag ctaaaaagga acaagcacaa
     4981 gttgaagaaa gaattttaaa attagaaaat atggttagaa aagctgttat catagatgaa
     5041 agcaaaatag accttaatgt tgttacaata ggatcaatag ttaaagttaa ggatttagaa
     5101 tttgatgaag aggttgaata tactatagtt ggttcgacag aagcagaccc atatgatggt
     5161 aaaatatcaa acgaatctcc tgtagggaaa gcacttttag gtagagctgc gaaagaagtt
     5221 gtggaagtgc aagttcctga tggagttgct aagtttgaaa tattagaaat aagaagataa
     5281 tttggaggaa aaaaatgaaa aataatcaac aaagcaatga agaagcgcaa attcaagaag
     5341 accttagtga agtacttcaa gtaagaagag ataaactaaa aaaattacaa gaatcaggta
     5401 gagatccttt taaagaaagt agatatgata gaactcatta ttctatggat ataaaagata
     5461 actttgattc tcttgagggt aaaactacaa aaatagcagg acgtataatg agcaagagaa
     5521 tacaagggaa agctggcttt atagatattc aagaccaaga gggaagaatc caatcttatg
     5581 taagactaga tgctatagga gaggaagaat atagtgtatt ttcaacatat gatattggag
     5641 atatagttgg aattgaagga gaaatcttta aaactaaaaa aggtgaaata tctgtaaagg
     5701 ctaaatctgt agtattacta tgtaaatctt tacaagtttt accagaaaaa tatcatggat
     5761 taaaagacca agaactaaga tatagacaaa gatatgttga cttaatagta aatccagaag
     5821 taaaaaatgc ttttttaatt agaacaaaag cattaaaagc attaagagca tacttagatg
     5881 atagaggatt tttagaagtt gagactccaa tattaaatac tatagcaggt ggggcaaatg
     5941 ctagaccatt tataactaat cataatactt tacatatacc aatgtattta agaatagcga
     6001 atgaattgta tttaaaaaga cttattgttg gtggatttga taaagtttat gaaatgggaa
     6061 gaatgtttag aaatgaaggt atggatttaa aacataatcc tgaatatact gcaatagaat
     6121 tatatcaagc atatgcagac tatactgata tgatggaaat aacagaaaat gttattgcac
     6181 atatggctga agttgctaca ggaagtatga tagttaacta tcaaggaaca gaaataaact
     6241 ttacaccacc ttggaaaaga atgagtatgg aagattgtgt taaagaatat tctggtgtag
     6301 atttttctac tataaataca gatgaagagg cactagaagt tgctagagaa aaaggtatag
     6361 agataaaacc tggaatgaga agaggagaag taataaatgc tttctttgaa gaatttggag
     6421 aagataaatt aatccaacca acatttataa ctcatcatcc agttgaagta tctccattat
     6481 ctaagagaaa tgttgaagac ccaagaagaa ctgatagatt tgaggcattt gctaataagt
     6541 gggagttagc taatgcattt tctgaactta atgaccctat tgaccaaaaa ggaagattta
     6601 tagaccaatt aagaaaaaga gaattaggtg atgatgaagc ttttgaaatg gatgaagatt
     6661 tcttaaaagc attagaggta ggtctacctc ctacaggagg actaggaata ggtatagata
     6721 gagtaattat gttattaact aactctccat ctataagaga tgtattatta ttcccgacta
     6781 tgaaacctat tgataacaat ccaaataaag aagaagaaaa ctaagaaata ttagcattct
     6841 actaagtttt aaataaagtt tagctattaa attatcaata aatagctaaa ttttatttaa
     6901 aatgtcagtg tagaatgctt tttatatgct tcaaagattc ttttagatat ttaatttata
     6961 aattaccaac aaacgttaga aattgatatt agtttttgtt gattgagact ttatatttag
     7021 aaaatatgat tgaaagtaat ttattatgaa aatatttaga ataatttatg agaaggtaaa
     7081 aagaataatt tatgagaata ctatatataa aaaattattg ggggaatgtc tgatgtttca
     7141 agtaattaat tttttatgtt tattttgtgt tggatttttg gttcttgatg ttatgatata
     7201 tttttgtaag gatgcaatgg atatttttga gggtacaact aatgttaata aagttaagag
     7261 tataaatgtt tgggcagata gagtgagagc taattcatat tcttatgaaa ataaaattgc
     7321 aaaataggta aaattatata tgcatgtatt gtttgtaata tcaagggtta tatctattag
     7381 ttttaattat atttattaaa aaaaacaaaa gttataacct tttttaaaat aaaaaacaaa
     7441 aaaagtattg acgaaa
//
LOCUS       DAMJWU010000139         7388 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMJWU010000139 DAMJWU010000000
VERSION     DAMJWU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.144"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            641..1150
                     /locus_tag="PC460_003342"
     CDS             641..1150
                     /locus_tag="PC460_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF2602197.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1178..1372
                     /gene="rpmI"
                     /locus_tag="PC460_003343"
     CDS             1178..1372
                     /gene="rpmI"
                     /locus_tag="PC460_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF2602198.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1428..1784
                     /gene="rplT"
                     /locus_tag="PC460_003344"
     CDS             1428..1784
                     /gene="rplT"
                     /locus_tag="PC460_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF2602199.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            2094..2639
                     /locus_tag="PC460_003345"
     CDS             2094..2639
                     /locus_tag="PC460_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602200.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            2626..2790
                     /locus_tag="PC460_003346"
     CDS             2626..2790
                     /locus_tag="PC460_003346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602201.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLLGLYFIVSFHLS
                     NDLEWYNEVF"
     gene            3079..5004
                     /locus_tag="PC460_003347"
     CDS             3079..5004
                     /locus_tag="PC460_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2602202.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKYTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            5405..7309
                     /locus_tag="PC460_003348"
     CDS             5405..7309
                     /locus_tag="PC460_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2602203.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKI
                     FGKEVNIITTSSLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3120 a          729 c         1382 g         2157 t
ORIGIN      
        1 ttataaagca tgtattgatt aagtattgaa taaaatatta caaatttagt tgaaatgaaa
       61 atgataaaaa atcttgtact atattttaca aatcatataa atagagaaat tgttgtagca
      121 caaagtagta ggtagtatat tagaatataa tataggattt gttgtgtagt atagtagcat
      181 attagaatat aatataggat ttgttatgta gtatagtagt atattagaat acaatataga
      241 atttgttggg tagtatagtg ttatattaga atacaatata ggatttgtta tgtagaatat
      301 atagagtcat gtagaatata aatatataaa aggattttgg gtataaattt aaaaattaat
      361 agcaaaatga gtagtataga catataatta gtagtaggaa gtaattaaat ttttcctaaa
      421 caaaaaaaat agttgcaaat aatttgatta agtgatatag tagataaggt aagaataaaa
      481 caaagaagaa gtccgcttct caccttacgc cggaggttag taaggtaaat ataagttaaa
      541 gcttaatttt aatttaagtt tgtttaaatt tggtaactta tattgcggat gattacgtca
      601 tccgcttttt attttataaa aattttggag gtgtcctaca attagcaaag aattagcaat
      661 caatgaacaa ataaaagata aggaaataag agtcctttca ccaacaggtg aacaacttgg
      721 agtaatgcct actaaagaag ctcaagcaat ggctaatagc aaaaacttag atttggttca
      781 aatctcgccg aatgcaaatc caccagtatg taagataatg gattatggaa agtttagata
      841 tgagcaagcg agaaaagaga aagaagctaa gaaaaaacaa aaaactatag ttgtaaaaga
      901 agtaagactt agaccaggta ttgaacaaaa tgatttaaat acgaaagcta ataatgctat
      961 aaagttcctt aaaaaagggg ataaagtcaa agttgaactt agatttagag gaagagaatt
     1021 aggacataaa gacataggta aagaagttat gcttaagttt ttagatataa taaaagagtt
     1081 tggagaacca acaaaagccc ctgcatttga gggtaataat atggttgtaa ttatagatcc
     1141 aaaaaaatag atgattaact gagaggagga atttgtcatg cctaaaatga aaactcatag
     1201 aggtgcagca aagagattaa agaaaacagg aactggaaag ttaaagagag ctaaagcttt
     1261 taaaaaacat atattaacta aaaaatctgc aaaaactaaa atgaacttaa gaaaatcaac
     1321 tttagtaagt gatggagatg ctaagagaat agcacaatta ttaccatact aagatacaga
     1381 ttgtaaataa taactataga agagggatat aaaaggaggt cataacgatg gcaagagtta
     1441 aaaaagctat gaacgctcgt aaaaagcata agaaaatatt aaaacttgca aaaggattca
     1501 gaggatctag aagcaaatta tatagaccag caaatacatt cgtaatgaaa gcattaaaaa
     1561 atgcttatat aggaagaaag ttaaagaaga gagacttcag aaaactttgg atacaaagaa
     1621 taaatgcagc tgctagaatg aatggaatat cttattcaag attaatgaat ggattaaaat
     1681 tatctggtat tgaagttaac agaaaaatgt tatctgaaat ggctatacaa gacccagaag
     1741 gatttgcaaa attagcagaa gttgctaaag caaaattagc ttagtattga atcatgagat
     1801 gtaaagtctt tgagttatat aaataattca aggacttttt tattttttga aaaatactta
     1861 ttagtgtcat gtacaaccaa ttttataatg ataaataaaa taagcaatac aatagaattt
     1921 tatgagattt attataagaa ttgttacaat tatttatgat agctataaaa agtcatagta
     1981 aaaatgatat tgatgtatgt tgatatatgt ataaagcttt caatcaatct tgaattaata
     2041 ttatcaacat gataatatta tcttattaat aatataaata agggggagat aatatgaatg
     2101 aaaaactaat ggattgtttt gtaaacccaa ttaaatgcaa gttattgatt gaaatttttt
     2161 caaataatcg aactacagct aaacaattag gagaaaaatt tcaagatata tcaaaggcaa
     2221 cactttatcg ttaccttaat aaaatgttga aagatgatat tataaaagta gttgaagaaa
     2281 ataaaataag gggaactgtt gaaaaagtat atgctatgaa gttagatata gctgatgatg
     2341 tgaacaaaat tatagatgag aactctggag aagaatatgc tcaactgttt actcaatata
     2401 tgatgggatt tttaagagaa ttttatgaat acgctgaaag aaaggaaaag gatatcctaa
     2461 aagatggttc aggattttct gtctgtccag tatatgctag tattgatgag atagttgaag
     2521 tgagtaaaaa aattgctagt atactagaac ctctaagaaa taactcgcac gaagaaggca
     2581 gaagaatgca ttctatagga gtgataatca ctccacctaa agaggatgaa gggaaataaa
     2641 tctgatttac atgaggtaga gccaatttac acggaaaatc gttttatgat ttacatatgt
     2701 atccagagca aagtatctct gcttggattg tacttcattg tatcatttca tctatctaat
     2761 gacttggagt ggtacaatga agttttttaa tttttcatat caatgcctaa agaaatgaac
     2821 tttagaccac atatgcaatt aggttacatt atacaattag accagatata caaaataaaa
     2881 atcagtattt ttatagcaga taaattgact gttattcttc ttaaaaatca tctattagtt
     2941 attgttagca tattgaaaga aattatagga atctattgaa taaatgaata ttgcccataa
     3001 tcatataaac tgctatagtt taaaataaag caggacgtta aattttatag taatctaaaa
     3061 atttaagagg gggattttat gaaaataaaa tttgttatta tgccattggt gatattttta
     3121 atggtattat ttgtggggtg tcaaagtaca aaagatacag aagttaaagc aaaatataca
     3181 gaaattaaaa tattaggtac aggtgatttg cattcaattt tgacagactc tatggtttca
     3241 tatgtaaatg aggaaaggga aaagaataaa aatctgctta tggtagatgc gggagatttc
     3301 tatggtacac aaagtagaga aatgtgggaa tggtcttctg gaaaaaagtt aattaatatt
     3361 aaaagaaatg gtagagcaga gtatgatgac ataattaaga gtagtaaaga tgaagtacct
     3421 atagttaaag atatggcaaa gttgaaatat gatgctgtag ttttgggaga taatgaattt
     3481 gtttcaaatg ataagcaaag tcttgataaa ctagtttctg attttaaaaa taataatatg
     3541 ccattggtat cagcaaatat atatgaacaa tctggagaaa attatgtaca accatatgta
     3601 atgaaaaata ttaagacaga tgagggcaat gtaaaagttg gaatactagg tcttactata
     3661 aaagaagtag gggaaaccct tggtcttgag gattttgaaa aggataaaaa agctagagag
     3721 cttggagaac aagctgatta caaaggaaaa ttatatgcaa atgatttagt agaagatgca
     3781 aagaaatgga taaaagttat ggaaaaagaa agtccagaca taatagttgc agttgtacac
     3841 tcaggggaag aacctaaaac agatagaaat ccaggaaata gaataaaaga gttagcaact
     3901 acagtggatg gaatagatgc aattgtagct ggtcatactc atgaaaaaat agatgaacac
     3961 aaatataaaa ataaatctgg agatgaagta attgttacac aaccaggttg gcatggagat
     4021 agagtatcag agattaattt caaattaaat aaagagaatg gaaagtggga cataaaggaa
     4081 aagtctagta aattaaaaat aattgataaa gaagtaagta tagttgctac tgcagattta
     4141 cattcacatt tctctgataa attaacagtt gaccttgtta atgaacgtag taacccaatt
     4201 gaacctgttg tagtagatgc aggcgacttc ttagaccctc aaacagatga aatgactcaa
     4261 tggtataaag agtggaaaag tataagagat aataatagtg ataaaacgat tagtagatgc
     4321 ccaatggtca tggatatgaa tcagggaatg tatgatgcag tagtacttgg aaatcatgaa
     4381 tttgtatccg aaaaagatga attttggtca gatgaacgcc ttttagatgc tgttatagag
     4441 gattttgaac aggtggcaat ttctgtgtta tcggcaaata tatataggca atctggtaaa
     4501 aattatgtta aaccatatat tataaaagat gtggagacga gtgaaggtaa tgtaaaagta
     4561 ggaatattgg gacttactat aaaagaagta ggtaaaggtc ttaaaaatgt aaacttacaa
     4621 gagcaatttc aatataagga taaattgtat gcaaatgatt tagtggaaga tgcaaagaaa
     4681 tgggtaaaag ttatgaaaaa gaaaaaacca gatataatag ttgcagttgt acattcagga
     4741 gaagaaccaa aaaatccaaa acatccagga aatagaataa aagaactagc aactacagta
     4801 gatggaatag atgcaattgt tgctagtcat actcatgaaa aaatagatga gcatgaatat
     4861 aaaaataagt ctggagaaaa agttatagtg acacaaccag gtgagcatgg taaatattat
     4921 tctaaaatta cctttaaagt aaataaagaa aagggttcgt ggattattaa tgataaattc
     4981 agtaaaacaa ttgaggttga ataactttat aaaatttata gataaagtaa attatctgat
     5041 attgacatga tatataagat aaagtttcta atattagtat aatgcataat gtgaaatatt
     5101 ttaataataa gagaataaac aagacaaata aaaatatttt aacatgtaag atatagtatg
     5161 aactattata ctaatgctta ctttataatt tgaacatgtt agaataagtc ttatttgaaa
     5221 attttagtgt attagagtaa gtattatttt accagtaaaa aatattgaga actatatgta
     5281 gatttgctta aaaaggaatt tacatatagt tcttttcgta taataaatag tacaatctta
     5341 tatggtaaag ataaagtgtg atattaaatt ttattatcat ccaaaatcta aggaggagtc
     5401 tcttatgaaa ataaaattta ttatagtagc atcaataata tttttaatag tattacttgt
     5461 ggggtgtcaa agtactaaag acacagaaat caatgtactc gccacaagtg atttacattc
     5521 aattattcca gataatttaa tttcctatgt agaagaggag agaaaatatg atgagaatct
     5581 acttatggta gatgcaggag atttttttga catgcaaagt ggtgagatga ataagtggtt
     5641 tactggtcaa aaattagtaa gatttaaaga tgggatacca gagtacaaaa aaatagctga
     5701 accacgtgaa ggagaagttc cattggctaa aaaaatggca aagctgaagt atgatgcagt
     5761 tacatttggg aatcatgaat ttgtatctaa tgacaagcag agccttgata aattggtttc
     5821 tgattttaaa aataacaata taccattagt atcagcaaac gtatatgaac aatctggaga
     5881 aaactacgta aagccatatg taatgaaaaa tataaatact gacaagggaa ctgtaaaggt
     5941 tggtatactt ggtctgacta taaaagaagt aggggaacgt tcaaggttta aaagttttga
     6001 acaagataaa aaggctagag aacttgggga acaacctcaa tacaaaggga aattatatgc
     6061 aaatgattta gtagaagatg caaataagtg ggtaaaggtt atggaaaaaa aagaaaagcc
     6121 agatataata gttgccattg tacactcagg tgaagagcca aaaaatccta aaaatccagg
     6181 aaatagaata aaagaactag caacaactgt agagggaata gatgctattg tagcaggtca
     6241 tacacatgaa aaaatagaac agcatacata taaaaataat tctggtgaag aagtaatagt
     6301 aacacagcct ggagctcatg gtaatagtat atcaaagatt aattttaagt tggataagaa
     6361 gaatgataag tggtttataa aagacaaata tagtgaatta aagatttttg gaaaggaagt
     6421 aaatataatt acaacttcta gtctgaatcc aaaattttca gatgaaatgg cagttaattt
     6481 gtttaatgaa agagatagag atgttcaacc tatatttata gatactggag attttttaaa
     6541 tactaataca aaagaaatga ctaagtgggc taaagagtgg caatacaatg aaaataaagg
     6601 catctataaa aaaataaatg aatggccaat tgtatatctt ggttatgatg ctgtagtact
     6661 tggtagccat gaatttacag aggataaaga gacttttgag gaaaaaaaat ctacattgga
     6721 ttatattata gaaaactttg aagaaatgaa gataccagta ttatcagcta atatatatga
     6781 tgaatctgga gaaaattatg taaaacctta tataattcat aatgtagaaa ctagggaagg
     6841 aaatataaag gttggagtat taggtcttac tactactgat aaggatgaag aagacttaag
     6901 tagctcaagc ttaagcaaag actcaaagac ttcgaaaatt cctgaagaaa caggaaagtt
     6961 atatactaat aatctagtaa aagatgcaag cgaatgggta aaagttatgc aaaaggaaag
     7021 tcctgatgtg attgttgcag ttgtacattc agataataat agtactctaa aaaatccaag
     7081 tagtgaagtc aaaaaacttg caatgtcagt agatggaata gatgctattg ttgtaggtca
     7141 tacgaaaaat aaggtagaag aacatattta caaaaataag tctggggatg aggttatagt
     7201 aacacaatct ggagaagatt gttattcaaa aataagtctt gaactaagaa aagaaaatgg
     7261 caagtggaat atcataaaca aatatagtaa agcaataaaa atgaagtgaa tttattgata
     7321 tataaaccat tattaacact gatttttata atataataaa tactgattct ataatataat
     7381 taacacta
//
LOCUS       DAMJWU010000140         7354 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMJWU010000140 DAMJWU010000000
VERSION     DAMJWU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.145"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(61..1008)
                     /locus_tag="PC460_003349"
     CDS             complement(61..1008)
                     /locus_tag="PC460_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2602204.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(977..1423)
                     /gene="lspA"
                     /locus_tag="PC460_003350"
     CDS             complement(977..1423)
                     /gene="lspA"
                     /locus_tag="PC460_003350"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2602205.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(1733..2512)
                     /locus_tag="PC460_003351"
     CDS             complement(1733..2512)
                     /locus_tag="PC460_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2602206.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(2567..3049)
                     /locus_tag="PC460_003352"
     CDS             complement(2567..3049)
                     /locus_tag="PC460_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2602207.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(3315..4736)
                     /locus_tag="PC460_003353"
     CDS             complement(3315..4736)
                     /locus_tag="PC460_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2602208.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(4882..5652)
                     /locus_tag="PC460_003354"
     CDS             complement(4882..5652)
                     /locus_tag="PC460_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2602209.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(5657..6859)
                     /locus_tag="PC460_003355"
     CDS             complement(5657..6859)
                     /locus_tag="PC460_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2602210.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         2453 a         1273 c          715 g         2913 t
ORIGIN      
        1 tcactaaatt tatttactta tataaaaaca aaaaatgatt tataagtatt atacaaatag
       61 ttattttatc tttttaataa cttccttaaa atactcaggt aattctgaat taaattcgat
      121 atattcctta gtagtaggat gtataaatcc caacttctta gcatgcaaag tttgtccttt
      181 taatttaaat ttagtttctt ttggaccata tacataatca cctaaaagtg ggtgatttat
      241 agacaaagca tgaactctta tttgatgagt tctaccagtt tcaagcctag ctctcatatg
      301 tgtaaactta tcaaatcttt ctacaacttc aaagtgagtg attgcatttt ttccatcttt
      361 tactacagcc atttttaatc tatccttagg atttctccct ataggtttat caacagttat
      421 tttatcttct ttaaccacac catagcatat aaattcatac tccctagtta tagtatgttc
      481 ctttaactgt tctgctagaa aattatgtgc ataattgttc ttagctatca taagaagacc
      541 agaagtatct ttgtctattc tatgaactat accaggtcta ataacgccat ttattgatga
      601 caacttatct ttacaatgat ataaaatccc gtttactaac gtattttcat agtttccagg
      661 agcaggatgg acaaccatat cctgaggttt atttattatt aagacatcat catcttcata
      721 tacaatatct attggaatat cttgaggtaa aacttctaat actttaggtt ctggtatctc
      781 tataacaata ttatcttcct ctttaaccag atatctagct ttttctactt tattattaac
      841 ctttactttt ccatctttta taattttttg tatataactt cttgacatat cacctaattg
      901 ttctgctaga taaatatcaa gtctaacttc ttcttcctct tcaagtacta aaaactcttt
      961 tacttcatcc attatatcac ctacttttac tttcaaaaaa taaaacatat atacataaaa
     1021 acacagttcc tacaactaca aatacatcag ctatattgaa tacatattcc catataattc
     1081 taaagtcgaa gtaatctact acaaaaccta atcgtactct atcaattaga tttcccaatg
     1141 caccagatat aattaatata attcccaacc ttccaaatat atgtactttc cttgtattaa
     1201 gatagtatag tccaaatact gttgcaagta atgcaacaat tataaatatc cattgattat
     1261 tttgtaataa tccaaatgct gcacctctat tttcgacata agttaaatga aatacattat
     1321 ttattattgg tattgttgat acatccacca aattattcaa tacccatatt ttagacagtt
     1381 ggtctaaacc tatgagtaga attattatta atatatatag caatgatttt tccccctata
     1441 aattatttaa aaaccaattt tatttgaagc acactattat tattatacaa aaaaaatgct
     1501 ctaaagagca actttattaa aattgtattt tcttaataaa gctagaacaa aatttaaaat
     1561 agtactactt tttttatatt gtagatttct acatatttaa aatcatatca ttatgtataa
     1621 aaaatttaaa aataccatac taatcaatat tttttgtgta ttttatagat taaagattaa
     1681 tgatgtctgt aatttaaact tacaatcacc actaatcttc aaacaaatat ttttactttt
     1741 gcacttgctt atttttatct aaatgttgta taagttcaga tactgttacg aatttatatc
     1801 ctctctcttt taatttagga attactactt ctaaagcttg tatagtttgt gatttatcat
     1861 ttcttttagt gttataatca tgtaaaagta ttatattacc attttggacc tttgtaagga
     1921 tagtatttat aattgcatct actccagggt tagaccaatc tctagggtct aagtttgacc
     1981 aaagaattat attcatgcct ttagaaacta taatatcaca gacactttta ctcatggctc
     2041 tatatggagg tctaaatatc tttggttcta ctcctattac actttttata atattttgcg
     2101 tatctgttat ttctttttca acactactat aactattttt tgcaacattt atatgtgtaa
     2161 atgtatgatt ccctatttca tgcccctcat catattgtct ttttaataat tctttgttat
     2221 attctacttt ttctccaact acaaaaaaag ttgcttttac atcatttttc tttaatatat
     2281 caagtacttg tggtgtaaaa tcctcatctg gaccatcatc aaaagttaat gctaaaattt
     2341 tttcatcctc atttcctttt tttattatta catcttcata atcgtctttt atattgtcat
     2401 ttaaactata tgcttctttt gaactatctt gattatgtgt attatgatta tatgtatata
     2461 cacctactat agataataaa catatacata taccccatat tattttcttc atgtcattac
     2521 ctcttttcta attcattcta gcaatatatt ttataatgtc agttttttaa tctctcaata
     2581 ttccattttt atttaaatca gttaaatttg ttaggtcttc tgaataaaag ttagaatctt
     2641 ctacatcagg gttttgcatt gcatcaccta ataaattatt ttctttcttt gcacaatcat
     2701 tacataaact tgtatctggc aatatatcca atctttctaa atctatttga ttatgacata
     2761 aatcacaaat cccatatgtt ccattctcta ttttagataa tgctaagtct atttgataca
     2821 atcttccctc ttcatgattt attagactgt tttgcatatt ttgcatatat aaatctgttc
     2881 ccatgtctcc tatatgatta tcataactag ataattctcc tgatgaatac ttttcacttg
     2941 tatgtttagt tgtatctcca aataaagtat tatctttcat atcctctact aaattagtta
     3001 aattttctct ttctttcaat aatttatctt tatatttttt agtatccatt tttaacacct
     3061 ctaattttaa tatttacatc ataaaaatag tatgtggaaa agtaaattaa acatacaata
     3121 aaaataagat ttgattcgaa aaatggaatg tctcaaaatg aaatttttag ttcatttaac
     3181 cctctttttt atatgaaatc aaatatattt ttatcctttc agcaataaaa aagagactta
     3241 tctctatttt gagatagtct cttctttgaa cataatttaa atacaatttt ttaattttta
     3301 tagttattat tttactaact tgccaattta tgattatttt gttctttaat atgttttgct
     3361 tttttaaaga ataaaagacc aagaattatt gctacaataa cacctattcc attagatata
     3421 ttaactggta gtctaaatcc ttcttttgct tgcattatat aactaaatgt tactacagtc
     3481 ataaaaagtg ctggtaatgt agttatataa tggtttttat ttgctttgac taaatatgca
     3541 gaaccagtcc acaacattat cattgcaagt gtttgatttg cccaagagaa atatctccaa
     3601 attatattaa aatctataaa tgtaagagca acaccaactg cgaataatgg taaagctatt
     3661 aaaaatctat ttttgactgc atcttgatta taattcattg catctgctat tgtcagccta
     3721 gcacttctaa atgctgtatc tccagaagtt ataggacatg ccacaactcc aagtatagct
     3781 aaagctcctc caataggccc cattaatcca acagatactg tatttactac tgttgctgct
     3841 ccaccatcat taaatatcac attaagttga ggtatgccat ggaaatatga catggctgct
     3901 gctgcccaaa ctaaagctat tataccttct gcaaccattg acccataaaa tactggtctt
     3961 gcctcactct ctttctcaac acatctcgcc ataagtggtg attgagttgc atgaaaacct
     4021 gatattgctc cacatgctat tgttataaat aagtatggga ataaaggtgt tccatttgga
     4081 ttcatatttt gtaaatttat atttggtatg ttatacccct ttattattaa tcctccagct
     4141 acacctactg ccataattaa taatgctgct ccaaataaag gatatatttt accaatcaat
     4201 ttatctattg gtaaaacagt agccataata taataaagta ttatcactcc aacccatatt
     4261 tgtgcgttta cacctgtcaa tgtctttaat aatcctgctg gacctgttac aaatacaact
     4321 ccaaccaata ctaaaagaat aacactaaat attctcattg ctatttttgc accatttcct
     4381 agataaaggc ctaccaactc tgatgcagat gtacctttgt ttttcattga tagatatcct
     4441 gaagcaaaat cgtgtactcc accagcaaag attgttccaa acacaatcca taaaaatgct
     4501 gatggtccaa ataatgctcc ttgtatagct ccaaatatcg gacctaaccc tgctatattt
     4561 agaaactgaa ttaaaaagat tctcttccat ttcattggca cataatctac tccatcccta
     4621 caagctatag ctggagtttg atttttgtca ttggttccaa aaaccttttc tacaaaagta
     4681 ccatagacaa aataacctac tataagtact actattgaac ctaaaaagct taccataaat
     4741 tcatcccctt agtttttata taattgcttt ataaattttt atgagtttaa gaaaactatt
     4801 tccagttcaa atagtaatac ttttttatgc taatataaat attaattaca ttaagtgcat
     4861 atttaagtac atgaaatgac tttatatatg cattaacagc ctaaattctt ttactttgct
     4921 tctacttaca gttacttcat aatcactatt ttttaatttt aatatgtatg tattattaaa
     4981 ccatggtata atttctttta ctttggttaa gtttacgata taagacctat gtgttctaaa
     5041 aaatgtttta tcatttataa gattttcgac ttctgatatg ccttctttta atataaactt
     5101 ttctttctca gtatatataa atgtctgcct ttcattagct tcacagtaat atatatcatc
     5161 aatatctatt acaactaatt tatcaccttt ccaaaggcta attttatgtg ttatttcttc
     5221 tatctcctga tttactgctt ccttcttata tttgtataaa ttactattta cattaaatag
     5281 ttcaatatca ctcatctcac ttttttctaa tttattcagc atagaaataa ttctctcttc
     5341 agaatatggt ttcaatatat aatcaaatac atccaaagaa aaagcatcta ctgcataact
     5401 ttcatatgct gttataaaaa caattttagg tcttgattta aactgattta atgtttttgc
     5461 aagtagcatt ccatcaagat gaggtatatt tatatctaaa aaaattacat ctattttatt
     5521 ttcctgaata aagtctaaca cctctatacc attggtaaat tcacttacta cctcgattcc
     5581 acttttattt tcaataaaat atctcaattc ctttcttgct ggaaattcat cttccactat
     5641 tatagctctc atacttctac tcctttacat aaaatactat caaagtccca ttatctattc
     5701 ttcttatatt aagccctctt ccatacatca gtttaagcct taaatgtaca ttataaagcc
     5761 caatcttatt ttctggcata ttatcttgat atacattatt tataatttcc tcacttatac
     5821 ctataccatt atcctctatt gatattctca cagtattttc taatgaatac ggtaactttt
     5881 ttatggatat tttcactaca ccagctctac cactttctaa aataccatgt attattgcat
     5941 tttccactaa tggttgtata atcaaacttg gaatctttat atttacatct tctatatcat
     6001 aagatacagt tagtaattct ccaaacctag ctttttctat ctctacaaaa gatttaactt
     6061 gttcaatctc tttatttata tcaattaagt tatcactata ttcaagatta tatcttagat
     6121 aagttgataa atttattatt aactctctag ccttatctgg actaaatctt ataaatgaag
     6181 ctattgtgtt tagggaatta aacaaaaaat gtggattaat ttgtctttga agtgctttta
     6241 tttctgcttt atttctcgct tctatatctt ttttatcttt ctcaatcccc tctactatag
     6301 atataacaaa tcctattcca atctgcccaa ctattaaagg taaatatatt ccaccgatta
     6361 tttgaattgc taaagaatgt ggatatgata ttaagtatat aagtaatata gtaaaacttt
     6421 cagaaatcat gcctaccaat attccataca ttactctatg ttgttttggt atcctcttat
     6481 atagaaatcc tgataataca cctgctagta tacttgataa aagacatgga acagatgtta
     6541 tccccccaat atccataaaa tatctatgaa tccctgaaaa aactcctgct gtaataccta
     6601 ccatcggacc aaataaaatg ccacctgata ctatagatat tattctagtg tttactattg
     6661 accccatata atttatacca ctataagttc caaatatggc taaaaaagta aaaactaatg
     6721 ctatacagac caagtcatta aaactatatt cttccttttg aaatatctgc ttaaatattt
     6781 ttaactttgt tattagaaga aacaatacta caataaggct tgtcttttct attaatgcga
     6841 ttaaaattga tgttttcata atctcccttt aaaaaataaa tttttacaca ctctaaattt
     6901 attatggttg ttataaatta catatttgaa gtaatcctat cacaaaactt tccacattta
     6961 atcttttaat taaataagaa atagttgtac ttctattaac aactaatact agctagcata
     7021 agcttgtaat aacttgtagt tattattaac tttacaattc tagtatttaa ttttatactt
     7081 tagaaatctc ttttcttaga aataatactt tcgaatatca tataatacaa tattatgtag
     7141 attcttactc atacaacata tttctttaaa aattttatac tattctttgt tcaaatatct
     7201 catttgattt aattgtgtta aacacacatt atcatctctc tgttatatat ttattatata
     7261 gccgtatatc gataaagtaa ataaatccca tatttaaaat aatatgtttg acaataaatt
     7321 attataatgt atatttacca taataatttg tcaa
//
LOCUS       DAMJWU010000141         7341 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMJWU010000141 DAMJWU010000000
VERSION     DAMJWU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.146"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            151..3330
                     /locus_tag="PC460_003356"
     CDS             151..3330
                     /locus_tag="PC460_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2602211.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPREYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3615..4379)
                     /locus_tag="PC460_003357"
     CDS             complement(3615..4379)
                     /locus_tag="PC460_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2602212.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4535..4768)
                     /locus_tag="PC460_003358"
     CDS             complement(4535..4768)
                     /locus_tag="PC460_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602213.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4842..6335)
                     /locus_tag="PC460_003359"
     CDS             complement(4842..6335)
                     /locus_tag="PC460_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2602214.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYNSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(6663..7205)
                     /locus_tag="PC460_003360"
     CDS             complement(6663..7205)
                     /locus_tag="PC460_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2602215.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT         2694 a         1059 c          817 g         2771 t
ORIGIN      
        1 ttcaataaaa atctaatagt aaattgttat ttacacatat attgtgatag ataaaagcta
       61 ttcatatctg ccttaatttt agtcttaaat ttagagtgta aattgaatgc atttagatta
      121 taatatatac agtgaggtaa ggtgaataat atgaaaatag atatgatata taaactaata
      181 agaagaaata cagaaccatc aagatggtca agaggtgtaa aatattatac taatcaatta
      241 gttgattata tagacataga tattgatgac ctacaaaata acctgttgac atttcgtggt
      301 ggagtcaatt ctgaagataa ttatagcttt tattttaatt caataactat agatataaag
      361 cactttaaga taattgaaaa tgattgtagt tgcgctgact gtcaatcaaa gggcaaaagt
      421 atttttatat gtaagcatat agttgctatt gtgataaaaa gcttaaaaca aataaaaaat
      481 aaaaaagatg ttcctcctat tctaatagat atattaggtg cagataagga taagctttct
      541 aatacaaatt ctaaaaccag cttagattta agcaagcaat tactttcttt gttagacaat
      601 actaaaagag aaagggttaa tttagatgta ttgataaatg tggattcaaa taaagcaatt
      661 gctgaattta aaataggaat tgataaaatg tacattttaa aaaacattac tgaatttgca
      721 tattcaaaac ttaatggata tgaattagat tttgggaaga aatttactta caaccccaac
      781 actcattatt ttgctgatga agatgagaag ttagtagata ttatagaaga atatgggaca
      841 aatctttcat acaacttttc ttcatcacat tcaaaaaaat ttatggagat aaatgcaagc
      901 tctttaaaac aatttttaaa tgctattaag tacaaggatt ttacccttat tttttctaaa
      961 gaggaatatc atccaaagat attaaatgat actctacctg taaggtttga ttttaaaaat
     1021 atagaagata aaatagtctt atattctaaa gattcactac ctgttccact tactaaaaaa
     1081 aatgatgtac ttttatatga tggcaatatc tatttattat cacataaaaa agctcatgat
     1141 tattcaaaaa tatatcaaat attatcaaag actaaagagt taaaatttga taaagaggat
     1201 tctaaggatg ttttaggatt gctagttcct agattaaaat ctatatctca agaagttcat
     1261 ttagatgata acataaaaaa taatataaca aaagatttta aatctgagtt ctactttgat
     1321 atgattgatg gcaacatatg ttgtgatgta aagtatatat atgatgatga agataaaaaa
     1381 tttgtgctcc ccaacataca aaaggaagct actattgaaa ataaacttac ttcttcacaa
     1441 tttacaaagg aaaataatca ttatgtgttt aaaggaactg accatgacct atttacattt
     1501 ttagatttac aattagaatc tctaaaggaa tttggagata tatattattc tgagaagttt
     1561 aaattgaaaa aaatatataa cgcttcatca attcaagctt caatcaatca gactaaccag
     1621 aattacctag aatttaattt taatatatct gatataaatc caagagagta caaggatatt
     1681 ttgaaagctt ttcaagaaaa acgtactttt tataaactta aagatggaaa ctttatagat
     1741 ttatcagaaa gagaaacaaa agattttttt gaattagttg aaaatctaga tataatggat
     1801 aaatcaaaaa ataataaaat tcataaaaat aaagctctat atataaatga tgtaatacgt
     1861 agtaaaaatt taaagtttat agatggtaaa aaagaactag acagtatatg tgaaaaattt
     1921 agaaattttg atagcataaa tttagaaatt cccgcaaatt taaatgcaaa ccttcgtgac
     1981 tatcaaataa atggcctcaa ttggtttaaa gttttagatt attataaatt tggtggtata
     2041 ttagctgatg aaatgggtct tggtaaaaca atacagacta ttgcattttt attatcatta
     2101 tctaataaaa aaagtcttat tgttacaccg acatcactca tttataattg gaaaaatgaa
     2161 tttgaaaagt ttgctccaga tataaaagtt ttattgatac atggaaataa aagagataga
     2221 gaaaaatgtt ttatggaatt agaaaatttt gatgtaatac ttacaacata tggtactctt
     2281 agaaatgatt tagaaaaata tagtgaaata aagtttgatt attgtatatt agatgaagct
     2341 caaaatataa aaaatccagt agctttagta actgagtctg taaaaagtat aaatgcagaa
     2401 aataaatttg cactcactgg tacacctatg gaaaataatc tactagaatt atggtctata
     2461 tttgatttta taatgcctgg ctatctatat agtaaagcaa agtttcagga attgtttata
     2521 aataaagaag ataatgttaa aaacctgaaa aaattgataa aaccttttat tcttagaaga
     2581 agtaaaaaac aagttatgaa agaacttcca gataagattg aaaaaaactt ttttgttgaa
     2641 ttgaataaag agcagaaaaa gatatatagt gtatattcaa aagatataca agataaaatg
     2701 aaagataaaa atctaaagaa agataaaata gttatatttt cttatttaac taaactaaga
     2761 caactttgtt tagacccaag tatagtagta aaagattaca ataaaaaaag ttcaaaaatt
     2821 gaaacatgtt tagaaatact tagggatagt ataaacgaaa accacaaaat attattgttt
     2881 tcacaattta cttctgtgct taagaacata agtaaggaac ttgataagta taaaattaaa
     2941 taccattata tagatggtaa aactaatgcc aaagaaagac ttgaacttgt agatgagttc
     3001 aataatagta tggataaaaa agttttttta atatctttaa aagcaggcgg aactggttta
     3061 aatcttacat ctgctgacat ggtaattcac tttgacccat ggtggaatcc atcagtagaa
     3121 aatcaagcta gtgatagagc tcatagattt ggtcaaaaaa attcagtaca agttataaaa
     3181 ttgattgcta aagggactat agaagaaaaa ataataaaat tacaagaaag taaaaaagag
     3241 cttataaatc aatttataaa tggagaactt tctaatgaag gtgtattaaa aagtctatct
     3301 gatgaggaga taataaactt atttaactaa attatttaat cctacagctt aactatataa
     3361 atttgttcta acatttatat agttaagcta aaatatctga ataaataaaa tttaatattg
     3421 ttatttaatc ccaactttac aatctaatga tatctctttc tatacatatt gcatatacaa
     3481 atgtaataac ttgactatta gaatattttg ttttaacaat actatttcat cataataatg
     3541 aagtatatgt acactcactt ttgtaagttt tgcaatccat tttacagttt tcattattat
     3601 aaattttggt taaattattc ctctaaaaat ttctttgcta catttgaaaa atctaatcca
     3661 ttcgataaaa ttgcaggatg accaccttta tcaaacaaat acattttacc atttttaatc
     3721 ttttcaagaa gtaatgaaaa agtattttca aaaaaatcac tagatattag agatacaaac
     3781 tcatcttctc tacttcctgt taaaagcacc tctggttgca tagtttctag aggcttatga
     3841 aaaaattttc ctatagtttt ataatgttca aaaatagctt ctgtatcatt atctacaact
     3901 ttttcccagt tttctccttg attataaata taaaaagctt ttgcaccatc atcttgctta
     3961 gatgcttcac gctctatttt cacattttgt acaaatgact ctagaggaac ttctccttca
     4021 aaactatctg caattacctt attcactaaa tcaggtctct caagtgcaac atttaatgca
     4081 acaagagctc ctccactact accaattatg tttacttttt tatagttcat tgcttctaaa
     4141 aaacatatca cttgcatagc ctcatcaaac cataagtcag cagaaaattt atcgaccatt
     4201 tgagattttc catgacctac aaaatcaatt aatattactt tatattcatc tttataaaaa
     4261 tcgattatat cattaaacat cttggaagag gctgtatttc catgtaaaaa aagtaatggt
     4321 gtaccatccc caatttcatt atagtagcac tgtttgtcgt tatagttaaa atatgccata
     4381 tatttccccc ctttaatatc cctataggta tatatttatt acttatattt aaaatgacta
     4441 ttccattagc cacttaaaaa tagtataact tagaatgtag ataaaatcaa cattccttgc
     4501 taatttatct attgtttgtc taatttatta taatttaact tctgtaaaat ttcttttttg
     4561 tacgaaaata ttccttccat ttacaacaat cattcaaatg aagtataaca tgacgagtag
     4621 gaggcataag caaaattcca gcaatatctt tattccccca aaagtccaac aaccaaatag
     4681 aatcttcatg ctttattact cctccagtca ccaaaatcct ttctaagtca attggagaga
     4741 cctttcgttt catatcttca gctttcaaat ccttaacaac ttctattgtg ttttttgcaa
     4801 cttcatttcg ataacaaagc aattcatcaa tattgactaa attatcgtat atctcttttc
     4861 actttaacca tttttttctt tataggtatc tccttatatt tttttaatat caaatctccc
     4921 ttataattta atatatctac atatgaacat aaatcttgaa cttcaattac acctatatca
     4981 tcattagtta taccttttat attactaaat gcaccaacta tatctaaaac tcttattttc
     5041 ttcttcttac cagcatttaa atataattta acaacttcac tttctatatt tagttttata
     5101 tctttactat tgtaagaatt tttatcaact ttttttctct ttgatacttt ttttaggatt
     5161 ccttttgaat attcttcaaa caaaattttg ccatgtgtaa ttctactttt ttctatatct
     5221 tttaattcat ttattttata tcctatatac gtttctatat tctctatata tttttcatcc
     5281 ttttcagtta ctatagtaat tgcctttcca ctatttcctt ttctcccagt tctacctatt
     5341 ctatgtatat agttttcttt atcttgtggc acatcataat ttattactaa agaaatatca
     5401 tcaatatgta ttcctctgga cgctacatca ctactaacta atacattaaa tttttggttt
     5461 ttaaaatcct taattacaaa aattcttctt tcttgtgata aatctgcatg taactctctt
     5521 attaaaaaac cttctttact catcttgtta tataatttag atactttctc tttggtatta
     5581 caaaatatta tcacactctt tggattttcg ctgtatatta attcttttaa aaggatgtac
     5641 ttctctcttc catttgcctt gattattttg tcatctattt gtcttgtttt ttgattagta
     5701 tcgctctcat tttcttctat atttataaca ctgtaatcta gcatatattt ttcacatata
     5761 tattttatct cttcatctat tgtagctgaa aaaagactta ctattttctc cttaggtaaa
     5821 tttaataata ttttttccat ttgttcaaca aaacctttat taaacatttt atctgcctca
     5881 tctattacta aatactttac attttccaac ttaatagaac ctctatttat atggtcaagt
     5941 attctgcctg gagtagctac aacaatatga accctttgtt tcaattcagc tatttggtct
     6001 tttatagatt gtttaccaaa tatagcacta catctcacct tctttagtct ccctatatct
     6061 gatatctcat cttttacttg caaggctaat tctcttgttg gaaccactat aagagcttga
     6121 atattattgt aatctacatt tatattctcg cataaaggta ttgcaaaact tgctgtcttt
     6181 ccacttcctg tttttgacct aactactaag ttttgacctt ttaataattt tggaataact
     6241 tctctttgta ctctagaagg tatattatat cctaaactct tcaatgactt aagtatcttt
     6301 tcatttaact taaatttttc aaatgtatat ttcatatatt accaccttat tatatttatc
     6361 ttatctatat atttttctac attatattat tataatacaa ggttctacta tttttatatt
     6421 atttattaga ctgaagataa aaatatatac tctataaacc tatagtttag tagcaaatta
     6481 attaaatttt tatatttaaa tacctcctta aatatactga taaatcaata ttttcaagga
     6541 ggtatctctt gtcaataatc aaactaatct atcttttgaa agtttaaatt atactaatac
     6601 attgtatttt tatttatatc taatattaaa aggtattaat caatacctaa atcaccataa
     6661 tctcacatat ttatatcttt attcaatata ccttctgtaa tcaatcgtgc tatataatca
     6721 taacctgatt catttgcttt gttacaatca ttcacattat cacagaaaaa actttcaatc
     6781 tgtattgcaa ctggatttgt atttctcaat atatataaat tttctctttt tttagctcct
     6841 ctattttcaa ataattctcc aagtctatta gatactcttt tagcatattc taatccttca
     6901 gtgccatagt aaagcacttc tgtaccacat tgtgttccat cagctttatt taaatgtagc
     6961 tctactaata aatcgtattt accactgttt acttttggaa ctctatagaa aaattcttct
     7021 ctttctgaca taaagtgcct ttctggacaa attattacat ctactgtatt accttgactt
     7081 ataagcatct cagctaacat aggagctaat tttttattat aattgtattc atcaactata
     7141 ccatttacac ctgtactttt accacctttt agtatactat gacctactgt tatgcatatt
     7201 ttcatttact tttttcacct tcttttaagc caatttttat ataatagtaa tataatttta
     7261 aagttattac taataataat atattaattg aagaaggtaa aagttctccg tgattatcat
     7321 ttttttaaca tatttgtctt a
//
LOCUS       DAMJWU010000142         7199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMJWU010000142 DAMJWU010000000
VERSION     DAMJWU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.147"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            84..425
                     /locus_tag="PC460_003361"
     CDS             84..425
                     /locus_tag="PC460_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2602216.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            576..989
                     /locus_tag="PC460_003362"
     CDS             576..989
                     /locus_tag="PC460_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2602217.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDHIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1191..1832
                     /locus_tag="PC460_003363"
     CDS             1191..1832
                     /locus_tag="PC460_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2602218.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1856..3109
                     /locus_tag="PC460_003364"
     CDS             1856..3109
                     /locus_tag="PC460_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2602219.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3155..3805
                     /locus_tag="PC460_003365"
     CDS             3155..3805
                     /locus_tag="PC460_003365"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF2602220.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3856..4452
                     /locus_tag="PC460_003366"
     CDS             3856..4452
                     /locus_tag="PC460_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2602221.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4465..5304
                     /locus_tag="PC460_003367"
     CDS             4465..5304
                     /locus_tag="PC460_003367"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF2602222.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKKVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5549..6703
                     /locus_tag="PC460_003368"
     CDS             5549..6703
                     /locus_tag="PC460_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF2602223.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7009..7185
                     /locus_tag="PC460_003369"
                     /pseudo
     CDS             7009..7185
                     /locus_tag="PC460_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
BASE COUNT         2992 a          676 c         1258 g         2273 t
ORIGIN      
        1 atcagtctta catatatttc cgatagatta tattgataaa aaacactaaa attaaattaa
       61 taaaagtact tggagggatg gaaatgaaag aggaaaattt tattaatgtt gaatacgagg
      121 ataaacgttt ttcaattaat agtaaagaag ttttgtacat agaaaagcaa gagtatgaag
      181 taaagattta tactgaaagt ggtaattatg aagttggaga aaatgctcct tgtataagaa
      241 aactattgaa agaagaaaat tttttcaaat gcagtaatag ttatattgta aatctagaca
      301 agtgtgaatt ttatctcagc aataattgta tttttgtgaa taataatttt ataagaattt
      361 taggttcaga aagaaaaaag aaattaaaaa agaaattaga aaaattatat gattatgaag
      421 attaatattg ggaggctgta ttttatacag cctctttatt tattgattgt attattaata
      481 aattttaaat tatgataaaa tataaaacaa gtaacttatt tcagaataaa atacttttta
      541 aaagaaaggg acgagatatg ggagattata ataaaatgct tgaattaaag aataacttag
      601 gtatcagtga aagaaagtta aaatatcctt gtatctataa acatttcaaa gggaaatatt
      661 atgctacaat gggtcttagc aaggctattg atcatataga aaacatatgt gaaatatatg
      721 gtaaagaaaa tttaatccaa aacagaaata aatataaatt agttattaga catactgaaa
      781 gagaagaaga tatctatgtt tatagggatt tagatggaaa cttttatcat aaaaaagaag
      841 aagatactaa tgatttagtt ttatacaaga ccttatatga tgatacagga atatttgcaa
      901 gacctttgga tatgttttta gaaaaagtag atacagataa gtatgtcaat tcaattcaag
      961 aatacagatt tgaagaagta tataaataat ataagagaaa tatgtttata aatttagaaa
     1021 aattctttaa cagtacaata atttgcagaa atacaaatga gatttattgt aaataatatg
     1081 ttagattaga ttataaaata gaaacaaagt aaagtattat atgatataat taagttttga
     1141 gaagtgataa tttatattta ataaaaaata ggcatatatt aggaggacat atgggaaata
     1201 ctttttatat agtaagacat ggacaaacgg attggaatat attgggaaaa acacaaggtc
     1261 atggtaattc tgaccttacg cctcaaggta tagaacaggc aaaagaactt tctgaagata
     1321 ttggcaagta ttcaattgat tatattttta gtagcgacct tgggagagca atgcaaacag
     1381 ctcaaatttt aggagataag ctcaatattg aagttcaaaa aactgaggca cttagagaaa
     1441 tgggattcgg agtttgggaa ggccttttaa taaaagaaat tcaaaaagat tattcagata
     1501 tatatgcaac atggagaaat gaaccacact tagtaaatat accagaaggc gaaactctta
     1561 agattataaa ggaaagagta gatgctttta taaaggaact aaatgaaaaa tatgataata
     1621 aaaatataat tcttgttaca cattcaatta cattgagggt tatgttgttg tcatttttag
     1681 aatcaggtat ggaaaatata tatagaatta agcaagataa tactgcttta aatatagtag
     1741 aatttaaaga ttatgggcct gttatagtaa aaatgaatga tacaagtcat ataaaaaatc
     1801 atgtaaagat aaataattct gcactggagt aggaatcaat tataaggaga aatttatgag
     1861 taaagttata gtagttggtg gaggagcttc tggaatgatg gcagctttgt cagcttcaaa
     1921 aaacaataat gaagtaatat tagttgagag aaatggagaa ttaggaagaa aacttagggc
     1981 aactggtggc ggtagatgca attttaccaa taatagagag atagaagatt tttttgataa
     2041 agttgtcagt aataaaaaat ttctctatag tagtttctat acatttacaa ataaagacct
     2101 tatttcgtat tttgaaagta gaaatttgga atataagatt gaagaagaaa atgaccacaa
     2161 agtatacact aaaaatgata aatctataga agtaatagaa gtattaaata aagatttact
     2221 caatcacaat gtaaagataa tgtataataa gaaagttata gatattataa ctgaagaaat
     2281 tgctctaaaa gatgattcta ataaggacaa gtcaaagtat ctaataaaag ggattatttt
     2341 agataatgga gataaaatac taggagacaa ggttattata tcaactggag gagtgagtta
     2401 ttcaaagacg ggttcagatg gttcaatgta taagattctt aaaaagcatg gacatacatt
     2461 aaacaaacta tatcctgctt tagttccact tacaatagag gaaaaatgga ttaaagattt
     2521 acaaggaata tccatgaaga atgtggaaat atcttgtaag attaagaaaa ggaaaatatc
     2581 aaaatctgga gacatgctat ttgcccattt tgggataaca ggaccttgtg ttcttataat
     2641 gtcttcttat attaacaaga ttattgaaaa ggaaaaggta gaattaaata tagatttctt
     2701 accaaattta agtactgatg aaatctctag tataatcaga gcatttccaa ataaaaatgt
     2761 tctaaataat cttaagcaaa tactacctca aaatttctta aaagagattt tttctctatt
     2821 atctttagtt gataaaaagg caagtgattt gtctaaggca gatgaaatta gaattattga
     2881 gtatataaaa aatatgaagc taacttgtaa tgggactaca ggtataaaca ctggtatggt
     2941 tacttctggt ggtatttcag ttaaagaaat taattcttct actatggaat ctaaattagt
     3001 taagaactta ttttttacag gtgaagtaat tgatatagat gcagaaactg gtggatataa
     3061 tttacaaata gcattttcaa ctggatatct tgcgggtata agtgtataaa tataattttt
     3121 taataattaa atatgaataa atagaggtgt atatatgggt aatttagtta tagctgtaga
     3181 tggtccagca ggagctggaa aaagtacaat agccaagata gtagcaaaaa agctaaatat
     3241 aaattatata gatacaggtg ctatgtacag agcagttact tataagtgtt taaaaagtgg
     3301 tattgatgtc aataatgaaa aagaagttat tcaaatagct gaaaattcgg atatagattt
     3361 taaagataac aatatatatt tggacaaaga agttataaat gaagaaataa gaacaataga
     3421 agtaagcaat aatgtttcta atgtagcaaa aataaaagaa gtaagacagt tgatggttga
     3481 agttcaacga aaaataggaa tgaaaaattc agtaatacta gatggtagag atataggttc
     3541 atatgttttc ccagatgcag attacaaatt ctttttagta gctactccag aagaaagagg
     3601 aaatagacgc tataaagaac tttgtaataa aggatataat actactttag aagaagtaat
     3661 tgaggatata ataagacgag atgaaattga ttcaaataga gaatttgcac cattggtaaa
     3721 agctaatgat gcactagaaa tagatactac aggaaaaact atagaagaag ttgtagaaga
     3781 agttgtatct aaaataaatc tttagttttt tatgcatcat aatgaaaata aatgtagtgt
     3841 tgaagggaga taaacgtgaa tttttataga tttgtaataa atatttttaa gggtttttct
     3901 aaaatatttt ttaaatatga agttataggt gcagagaata ttccagatag aggaaatata
     3961 gtaatagcat caaatcataa atcgaatctt gaccctatat tcttggctgc tgccatagaa
     4021 aatagagaaa tagctgcaat tgcaaagaaa gaattgttta aagtaaaacc attgggattt
     4081 atattaaaaa aattgcatgt tatgcctata aatagagaaa aaccagatgt atctacaata
     4141 aaaactattt taaggtcagt tagagacggt tatgtattag gaatatttcc agagggtact
     4201 agaataaaag gagattcttt tggtaaggct aaggcaggtc tttctgtatt tacaataaaa
     4261 agtaaatcta aagttgttcc agtatcaata atatctaaat ataaactttt tagtaaagta
     4321 attgtatata ttggagaacc aatatcattt gaagaacatt ttaaggaaaa attgagtaat
     4381 gatgaccatg aaaggatatc acaagagatt ttagaagtga taaaacaaaa ttactttaaa
     4441 tattcaaaat aagggggtaa agttatgaac ataaaaatag caaaaaatgc tggcttttgt
     4501 tttggagtaa aaagagcaat gaaaatggca tgggatgaag ttgaaaaaaa tgattctggc
     4561 atatatgctt taggtcctct tattcataac aagcaagcag tagcaaaata tgaagaaaaa
     4621 ggtcttaaga ctgtgaatga aattgataca ataccaaatc atgaaaatat gataataagg
     4681 tctcatggag ttcctgaaaa tatatataag gaagccaaag ataaaaaatt aaaaattgtg
     4741 gatactacat gtccatttgt aaagaaaata catacagtag taagtgaata tcataataaa
     4801 ggctatgaaa taatagttat tggagatatg aagcatccag aagttatagg tataaatgga
     4861 tggtgtgaaa attcagctat aataataaag accttagaac aaatggaaaa tatggaattt
     4921 gataactcca aaagatattg tcttgttgca cagactacaa taaatccaga gttgtatata
     4981 agtatagtta ataaactatc agataaatta gaagaaatag tatttaatga tacaatttgt
     5041 tcagctacaa aaacaagaca agaatcagct aaagagttag ctaaaaaagt tgattgtatg
     5101 atagttatag gaggtaaaca cagttcaaat actcaaaagt tagtaaaggt ttgtgaagac
     5161 ttagtaccaa cttttgcaat agagactaaa gatgaattgg atgtaaatac gcttaaaaaa
     5221 tataaaaatt tgggtattac agcaggggca tctacaccta attggataat agaagaagtt
     5281 gtaacattcc ttgaaaattt ataatttgat aggtaataaa aataggtgga cattgttata
     5341 atttatcata atatccacct ttgatttcat attacatgtg gtaaatagat aacctaatat
     5401 caaaaagtgt gtatcttaag atgaaaaact aagagtataa gatgcgcact ttttaatatt
     5461 agaaattcaa aagaaatatt gatattgatt atcaatagaa aatagaatat aatataattg
     5521 ttgcaaaatt agtctaggag gtaaattaat ggaaatgttg atatccgctt ctgaagaggc
     5581 gtttttacat gtaggttcta tgattggatt ttttattttg ttatttggat atattaatta
     5641 taaaactagt ggaaatttta ctaatatcat atcaaaaaac agaaaatttc aacccttaat
     5701 aggtgcttta attggagcta taccaggttg tggcggaagt ttggcaataa tgccattgta
     5761 tataaatgga aaattaagct ttggagcaat aattgcaagt ttaatagcta gtatgggaga
     5821 tgcagcattt gtacttattt catcaaatat taaaatgtat ttttttgtca ctatagttag
     5881 tacaattaca ggaataataa ctggacaatt agtggattac tttaaactag aagaaaaatt
     5941 agggttaaaa aaccgtagaa aatcagataa atatgatata agtaaaaata accctaaaga
     6001 aaataaagaa caacataatc atgatgaaat aattttagac actctagcta aatctcatgg
     6061 aaatacaaat agacttgcat ttataataac acatggaaag ggatataaaa tttatatagg
     6121 aattatagta atcggtttta tatttatgtc acttgctcac tctggactta atcttcctat
     6181 aattgagaag ttacattcat tggaagaagt aatagcagtg attggaatat tattttcaat
     6241 aatttatatg tggtgtttta aaaaggtatt taaaaattct aatcaaaaag aggaagaaaa
     6301 taaaaagatt tctttgcgag aaatgcttat ccattctgtt ggagaaatat catttgtaat
     6361 tacatggatt tttattgcat atcttattta cgatttgata atattaattt taggtggaga
     6421 tgaatactta gttaaattag tattatcaac aggtgtaata agtgtattta taggtgcagg
     6481 tcttggactt attccaggtt gtgggataca aattgtgctt atgtcatttt atttgaaggg
     6541 aactatacca cttggtgcag tgatagccaa ctctatttct caagatggag atgcactttt
     6601 cccactttta gcaatggata aaaaatcttc tttatgggct atgattatta ctactatacc
     6661 agcaatactt gtaggtatga tagtttatgc atttttaggt taaatataat tatgaataaa
     6721 gaagtattaa aaaaataata aagcacataa atttatttat aagaatagtg aagattaaat
     6781 ataaaaataa aaaattcaat atgaattaaa ttattgctta aatattaatg agaacatggg
     6841 agataatatt attcctcatg ttctctctat ttaattaaag gaattatgga acaaatttca
     6901 agtgatactt tctatcaaaa ctcatatata atatatatta ataaattaaa ctataatacg
     6961 taatacagtc agtatattga tataaaaaac ttaggaggag ttgtaaaaat gggtaattta
     7021 aaaagacagt gtgatgtttc aagtggaaga gaaaaagctg acattgtact aaaaaatggg
     7081 acaatcataa atgtatttac agaagagtta ataacaggag atgtagcaat agtaggagac
     7141 actatcgttg gaattggcga ttacaagggc aatgtagaaa ttgattaaaa aatagctac
//
LOCUS       DAMJWU010000143         7176 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMJWU010000143 DAMJWU010000000
VERSION     DAMJWU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.148"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            151..1044
                     /locus_tag="PC460_003370"
     CDS             151..1044
                     /locus_tag="PC460_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2602224.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1222..2046
                     /locus_tag="PC460_003371"
     CDS             1222..2046
                     /locus_tag="PC460_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2602225.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEINQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2145..2753
                     /locus_tag="PC460_003372"
     CDS             2145..2753
                     /locus_tag="PC460_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2602226.1"
                     /translation="MGKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            2857..3033
                     /locus_tag="PC460_003373"
     CDS             2857..3033
                     /locus_tag="PC460_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602227.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4299..5051
                     /locus_tag="PC460_003374"
     CDS             4299..5051
                     /locus_tag="PC460_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602228.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            5041..7152
                     /locus_tag="PC460_003375"
     CDS             5041..7152
                     /locus_tag="PC460_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602229.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         2674 a          947 c          951 g         2604 t
ORIGIN      
        1 tttagtgtaa aaataattaa tcaaaatata aaaaatagga tattattata ggcaataact
       61 aacatcaagc gataacttaa tacatataaa aataaataaa caattaataa aaattaaaac
      121 ttcattataa aacggaagga tagtgaaaaa atgcaactat ataacacccc ctttaataca
      181 aacaaacact taatctattt ctttacattt acgctagtat tgaactggat attcagtctt
      241 acacctgcgt tgctcggaat tacaaataca atacttggag aaatcatttt ttatcttgca
      301 tatacatcac cagctattgt aggtctattt tttatttcta caatctatcc acaaagtgca
      361 aaacaagatt ttatttatag atacctaagc tttaaagaaa ttagaataaa atggatacta
      421 ttaacaatat tattttttgc tttaactttt attacttcgt taataatcgg aaattacttt
      481 aatgtaaaaa cttctgatat agattggata cacatagcag tgtttagacc ttctaaaata
      541 gcttatatgc tattagcaag ttttatattg agtatactta gtcaagaagc tggatggaga
      601 ggatatgcca ttgacaaatt attagttaga tttggattta taggctcttc aattatttta
      661 gggcttgttt gtggaatctg gtatctaggg tcgtatttta cacctaatca aataccatat
      721 aatttggcac aatactccct atttgatgct tttttattta taccaaacat tgtactttta
      781 aactttataa ttaattttgt atatatcaat acaagtagaa gcattttatc agctgggtta
      841 gttcatatga tgtactattt tttcaacatt caattacttc tacactaccc tattaaatta
      901 tgtgtaatag ctcaatatgt acaaataata tttggtttaa tatttcttgt atatatgatt
      961 tcttctaata aatttaaaca gaaggtaaat tctgaaattg aacagataaa gtcggatgat
     1021 tttgaatttg agttagactg gtaattttaa tatgaattat aaattaagat aatctatgat
     1081 ttgatacgat tactacttaa atataattta ctctctataa tttattgcaa catctggaaa
     1141 ataattgtat ttccacaaac gcttgaagta tataaaaaca aaatatatac tgattataag
     1201 tgaaaaggag gtcatatttt catgtttaaa ataggagagg tttcaaaact aacacaaatt
     1261 tctattcgta tgttgcgtta ttatgatgaa cttggtattt taaaacctgc agaaatagac
     1321 aaatatacag gtcatagatt atactcagtt gaacagattt caatactaca aagaattgta
     1381 ttattgagag attcaaaatt tagcgttgca gaaatagcaa atatcgtaca taattggaat
     1441 gatgaatttg taattaaaga attgaataga aaaaagaatg aaatacaaaa ggaaataaat
     1501 caggaacaac aaagaataaa taaaattgat aaatttatag aagctataaa ctgtgataaa
     1561 gatgaaattc attacaatgt tgtctttaaa aagattccaa gttataaaat catatcactt
     1621 agagagataa ttccagacta ccaatctgaa ggtatactct gggaaaagct atctaaattt
     1681 atcaaagaag aacatataga agtatcacgt caatccaaca ataacatagc tttttaccat
     1741 gatgaagaag tcaaagataa tggtgtagat attgaagttg gaatggttgt aaagaagatt
     1801 ggaaagaaca aatctggatt tatatatcgt gagacagaag aaattgatat gatggcatgt
     1861 actatggttt atggtccata tgaaaatata gctggtgcat atgaatcgtt ctgttattgg
     1921 ttagataaaa atagtgatta tcaaatatca ggtataaata gacaaatcgg acataaaggt
     1981 gaacataatg aaataaatcc tgaaaattat cttacagaaa ttcaaatccc ccttataaaa
     2041 gtctaataga ttagtactta aatacttaag tactaatcac ccattgacat cccaaaattt
     2101 tcatgatagt atctaaacgt gtgttaaaca tgtttaattt ttttatgggg aaacttttaa
     2161 ggggaaaaat tatgaaagca gattcaacaa aggaacaatt aattaatgcc acaatccagt
     2221 tactatcttc acatagagat gtttcaagca ttacggctag agaaattgta gctaaagcaa
     2281 atgtcaattt agctatgatt aattattatt ttaaatctaa agaggagtta attaatattt
     2341 ctattaaaaa atttttaaag cgtcatatgg atgaatataa ggctacacat gatactaaaa
     2401 atttagcacc taaacagcaa ttaagggaaa tactaattgc attttttgat tttataggtg
     2461 aacatagtca atttataaag atttctgtac cctatatatt gatacaggaa gaaattgttt
     2521 atccatttga gatactacct ttaattaagg ctcactatga aaatcagaat aacaaaaaaa
     2581 ctgaagctga atacagaact attgcgtatc aaataacttc atttattcag ttgctttttc
     2641 ttcgttctaa tgcttttttc aagtatagtg gtattgatat tcttaatatt gaacagagaa
     2701 atggtttaat agattttcaa ttagatttat tattggtaga tattacaaaa taatactata
     2761 ttaatttttt atgtacattt ttttatttta tggtaacgtt tgaaaatatt ctcattgact
     2821 aaaaatactt gcattaaata agggggagga attttaatgt tttttacatt tataggagta
     2881 atatcagcaa ttttattgtt gtgtacaatt atatgtggtt tatggataaa acttaataat
     2941 cgtaataatg actctaattc tcattttact ttttcaatac tttcaattgc tttttcactg
     3001 ttaattgtgg tggccttttt agtaagaaat taatgtcttt gttatgctga ggtttattat
     3061 tatagatttt tattattgca tgatttttac tatattatag tatttttatt atattacagg
     3121 gtcttattta ttatagtgtt ttgttgttac agcattttat tatattacag agtcctattt
     3181 attataggat ttttattata ttgtaaaatc ttatttatta taggattttg ttatattaca
     3241 gacttttatt atattgaaat ctcttattta ttgcagtgtt tctattatat tgcaaactct
     3301 tatttattat ggtgttttgt tattgcaaca tttctattat attgtaaagt cttatttatt
     3361 acagttcttt attatactac agacctttat tattacagca tttctattac attgtaaagt
     3421 cttatttatt acagttcttt attacactac agacttttat tattacaatc tcttatttat
     3481 tacagttctt tgctatacta cagaccttta ttattacagc atttctatta cattgcaaag
     3541 tcttatttat tacagttctt tattatacta cagactttta ttattacagc atttctatta
     3601 cattgcaaag tcttatttat tacagttctt tattatacta caaaccttta ttattacaac
     3661 attttattat tttaacttct ttactataat atagtaatat tttaatatct caaaatcata
     3721 tcaatcactt ttcagcatag tctaaaatac ttccactttc taaaattatc tatcatacct
     3781 atactaataa tactttttaa cctttttatt atacatttca attgtttcta acaacctatt
     3841 tatttccaaa actccaatat attttctcaa ttacattgtg ttaatagccc ctaaatcctt
     3901 tcaaattatt tacaatacat tctattaaat aatcttactg ctctataatt ctttttttct
     3961 ttttaaccct actatacatt atttacatca tttttttcaa tacaacattc gttattctat
     4021 aaccttttta cttttactat atacttcttt tctaactaca ccttcaatgt tccacatcat
     4081 attactttag cacaacaatt aatacctaaa aataaccctc taaatcctcc aataatataa
     4141 tcaatttaac tataataata acctctcaca ctatccaaac aatatatttt caaacattac
     4201 aaaaaaacat ccaactatta caatatagct catatccctc ctcccctttc aaatgctatc
     4261 attgacttac aagataaatg aaagtgagga tgatatatat gcttaaagta aaccaattat
     4321 gtaaatcata taaaacaggc agtaaaacat atcctgtatt aaaaaatatt tcttttgaaa
     4381 tcaacaaagg agaatttgtc gcaatcatgg gttcatcagg aagtggaaaa actactcttt
     4441 taaattgtat ttcatgtttt atcccaattg ataatgggga aataattctt ggaaataata
     4501 atataagaaa tttaaaagaa aaggaacttt cagaagttag aaatcaacaa ctaggatttg
     4561 tattccaaga cttcatgcta tttgatggtc ttagtgtctt tgaaaatata tgtgttccac
     4621 aaattatcaa aggtagtcaa gtcttacaaa tggaaaaccg tgcaattaat ctatgtgagc
     4681 tatttggtat tgaacatatc agaaaaaaat accctgccga aatatctggt ggagaaaaac
     4741 agcgtacagc agtagcaaga gcactcatga acaacccaga tttaatatta gcagatgaac
     4801 caactggtaa ccttgatagt aagtcttgtc gtacagtcat tgaatcattt ttaaaagcaa
     4861 agcaagaatt agatgctact atactcatgg ttacacacga tagctttgca gcaagcttct
     4921 gtgaccgagt tattgtgctt aaagatggaa taatttataa agaagttgta aaatcaaatt
     4981 caagacataa attcctagat gaattactag aagttttaaa agaaatggga ggcgctcaag
     5041 atgacaatta ataaattata cagtaaactt cgagcctata attggaagaa ctatacaact
     5101 ttattctttt gtataatact ttctgtggta ttagtaactt catatgctct aatttatttt
     5161 agcccaacaa tacaaaacat acttccaact ggtggagata gcagaaaaca ggctacaatg
     5221 atattctcag ttgctatatt aggatgcgct gtctttacta catacgcttc ttccctcttt
     5281 ttaaaattta aaagcaagga aattggtatt ttcatggctc taggaacaga taagaagaaa
     5341 ttaaaaaaac ttctattttc agagatattg ttaatttcaa tagtatgctg ttttttaggt
     5401 cttatattaa gtattcctgt atcttttggt atatggaaaa tatttcaatt agtgattatt
     5461 gataccagag aaatgacata tcattttgga actcaaggat tcatatatgg atttatattt
     5521 agtgtatttg tgacattatg catatttata ctttctgtaa aatttattaa tcgtacaaat
     5581 ataattgata tattaaatga ccagagaata tctgaaccaa tacaggatgt aaaggactgg
     5641 tatggtatat taggtataat ttttattata ctcggtattt ttctaggata tctcgtacct
     5701 tcactaacta taaacaaatt aaattataaa ctaccatcta tatggagctt tacttatcta
     5761 ctctctctta ttggaatata tatgattatg acatacatag ttgtacatgc taaaagagga
     5821 aaacatccag agaaatacta taaaaacatc atatcaacta gcatgatgag atttatggga
     5881 agacaaacag taaaaaacat gtgtgttatt tcatttttga ttgcaggagc attatttgct
     5941 gtattctatg ttcctaatat ggttacaggt atctatgata ctcttaaaaa taaccctata
     6001 gattatactt ttacctacaa tcaaagagat aaacaggtat caaaaaaaga tattgaacaa
     6061 ttagcagata aacacaatat aaaaattcaa aactatatgg aaattccttc tatagaatta
     6121 attgttgatg gtgtagaatc aatatatcat aaagatggaa ctatcgaaaa ggtatatatg
     6181 aaaaaaagct cctatgatat gttctttaat tctaaagatt tttccaagct aattggcaaa
     6241 ccaataacta taaaaaatgg tgaatatctt gctgttgtaa ataaaggaaa tgaaaatcaa
     6301 gattataaat ataccaaatt tatatcaagc ccagtcacag aaaaagaaat cccctttaga
     6361 tatgctggaa aaataatatt taatggttct ttttttcaaa atagaagtat gaacgcctat
     6421 gtccttaatg atgaggatta taaatcattt actaaagatt tatccataaa aaattacaat
     6481 aattctatac tatttaatgt agataaagat acctatgctt ttgccaatga cttaaagaaa
     6541 gaaattataa aaagaacatc taaggaaatg ggcatgttca gtggttatga tgaatacgta
     6601 aaaaaacgtt atgaatcaac tggaagaaaa tattttatgg atgaagaata tccaagtggt
     6661 gaaggtcatt taaaactaga cccaaacaac aatcagttgt atcttaattg gaaatactat
     6721 cctaacttta aaattttaca aagccaagat atgataaaaa acatggcagt cttcttaatg
     6781 ttattcatat atgtggcaat catatgtttt acagcagtag gaatcattgc atatacaagg
     6841 ggtgttacta tagctataaa tcacaagcag gtctttatgg atttaaatcg attaggagca
     6901 aacaatgatt atataaagtt ctgtattaga aatcaattaa agaaagtatt ttcataccct
     6961 cttattgcag gaagtttttt aatttatctg tttaatttta tgattatgtt aaataatgat
     7021 ggtagaatcg tcaatagtga ggtcaatgct cttttgataa acttaggttt tatagggatt
     7081 tgtggggttt atatgtacct tatatatcta ttaacttata agaaattcaa aaaaattatt
     7141 ggtattttat aataaaaaag gttattagat ttttaa
//
LOCUS       DAMJWU010000144         6938 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMJWU010000144 DAMJWU010000000
VERSION     DAMJWU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.149"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            84..1487
                     /locus_tag="PC460_003376"
     CDS             84..1487
                     /locus_tag="PC460_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2602230.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1706..4183
                     /locus_tag="PC460_003377"
     CDS             1706..4183
                     /locus_tag="PC460_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2602231.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4198..4884
                     /locus_tag="PC460_003378"
     CDS             4198..4884
                     /locus_tag="PC460_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602232.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5158..5991
                     /locus_tag="PC460_003379"
     CDS             5158..5991
                     /locus_tag="PC460_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602233.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            6029..6490
                     /locus_tag="PC460_003380"
     CDS             6029..6490
                     /locus_tag="PC460_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2602234.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            6521..6769
                     /locus_tag="PC460_003381"
     CDS             6521..6769
                     /locus_tag="PC460_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2602235.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2675 a          750 c         1076 g         2437 t
ORIGIN      
        1 aataaatttt tacatatcaa taattattgt ctattttata taagcaataa tcaaaataaa
       61 agtttaagaa aagagttgat taaatgcata aacctagtta taaaacagta tttcattcat
      121 acattatttt tttcatgatt tttatttgct taatcctgac agtaataggt attttcttat
      181 cacttattac tattcagaaa ccaaatgggg aatttattcg tagtgactgg ccgaaaaaat
      241 ttacagaaaa ctttaatgaa caaatcgtat ttatagataa taagccacaa gtaaaacagt
      301 ctggattaga aaaacttcga gaaaatcaat tgtggttgca aattttagat tctagtggaa
      361 atagagttta tgggttttta gaaccaaaag agcataaaga ttattattct aattctgaat
      421 tattagcatt agctaaccaa aataattcaa gtaatggaac tgtatacttt aatacaatta
      481 aaaataataa aaatgactat atttatatta tatattttcc attaaatata tctaaagtga
      541 atatgttttt tgatggaggt aaatttatag gtggtaagtc aattgtattt tcaatcatag
      601 ggattatatt tttaataatt ttggtttctg gaattgctta tggttactgg gtaacaaagt
      661 gcatatcaaa tattacatct gctgttagcg atatttcaaa acgaagctat cttccagtta
      721 atattaaagg tgtttttgct gatatatata atagtttaaa tattttaaat gaagaaattc
      781 atagaagtga tgagatacaa aataaaactg atattatgag agaagagtgg atttcaaata
      841 taactcatga tttaaaaact ccactttcac caataaaagg gtatgcagag cttcttacag
      901 atagtagtac taattattca cctgaaaaag tatataaata tgcttcaatc atgttaaaga
      961 atatagaata tacaaataca cttataaacg atttaaaatt aacctatcaa ttaaaaaatg
     1021 gaataatccc tttaaataaa aataattatg atttagtaag atttttgaaa gaattatcca
     1081 ttgatatttt aaactatcca gaatatgaat caaatatagt tgaatttaag agtaatatag
     1141 ataatattaa ctttgatttt gattgtacac tattaaaacg agcttttagt aatcttattg
     1201 tcaatgcttt tacatatgga gataaaaata ctaaagtttg tgtacatata acattaaatg
     1261 aaagaattga aatcattata tctgataatg gaaaagggat gtcaagagaa gaagcatcaa
     1321 atttgtttaa tcgttattat agaggaagca atacaaaaca aaaaatagaa ggaactggtt
     1381 taggactagc aataacgaag caaattattg aagttcatgg agggaatata tctgttgaaa
     1441 gtatacctgg attaggtagc tcattttata tcacctttcc tatttaatta aggttaatta
     1501 aggtcaaata aaaaatatat taaggttgat agattattat atttatatag tagtttatca
     1561 atctttttta tttaaggggt gatttgatta aaaatattat aaatagaatg ataaaagtat
     1621 ggaggagtct attagagatt taatttagat taaagtataa aaatattaga ttgaaatatc
     1681 ttagataagg aaagaaggtg taaatttgaa aattatatta aagtatattt tcacaaatat
     1741 taaagaacga aaaattagaa cagcagttat gttattgtcc atagttttat caacagtttt
     1801 actttttgtt tcattctcaa ttggattatc ttatgaaagt gcacaaagaa aaatggcaaa
     1861 aggtatgtat ggtacagcga ctatatctgt ccaaagtaaa aatccagata tattaaccaa
     1921 tttagaagac atacctgatt taaatgctat aaaatcaaag gttggtgtat tggaaagctc
     1981 tgctatttat aataaagggg gatactatga agaatttagt cttatatcag cagacttaag
     2041 tcaactaaat aaaatcaaca agccacgatt agaaaatggc gatagtatta ctgatttttc
     2101 tggtgataaa attattcttc caaatcgctt tacatcaaaa tataaaataa aaaaaggtga
     2161 ttctattact ttgcagattt atggaaagtc ttatactttt caagttagtg atattgcatc
     2221 ttatgataca gtatttttga ggaacacaag aggtgtaaat gcactgctac ccaaagagac
     2281 tctatcaaaa attataaata aaggcagtgg atatacaaga gttcttatag agtcagaaga
     2341 agaaattaca gaaaatctag taaataaatt atccaaagaa ctttctactg aaaaatatat
     2401 ggtatcaaat actataaatg aaactaagat aatttcagat gctagacaaa agacaatgcc
     2461 tttcttttta ataagttttt ttgcattaac ccttagtata tttataatat acagcagtta
     2521 taaggttatt acattagaac gtcttccttt tattggaaca tttagaagta ttggtgccaa
     2581 tgaaaaaact gtaactcgta tattgatgct tgaaagtata ttatacggaa gtataggagg
     2641 attaattgct attcctattg gtgtagttgt tttaaattta atgcttcatg ggttaggaaa
     2701 ttctctcgaa caaggaatat caattccagt agtaatatca cccattggag tgattatatc
     2761 tgttatagta gcaataatcg tttcttcatt tagtgcatat atacctgtta aaaaagctag
     2821 ccatttgccg ataaaaaata ttgtactagg aacagtagaa gaaaagaatg tttcaaatcg
     2881 ttctatactt tttataggaa gtataatgtt tattttatct attttacttc caagaatatc
     2941 ccctgaaaat acattgtatc tagctggtgg attttcatta ctgggattaa ttgtagctac
     3001 tattgtgttg attcctctta ttactgatat catgtctata gtttttgagt ttgtttataa
     3061 aaatatttta gggaatgagg gtaaattagc tgctagaaat atgaaaaata acaaaaatat
     3121 aatacagaat atcactttac tatttataag tatttctgca gttattgcaa taagtgttgt
     3181 tggtaacttt gtcaaaactt atataacaga tgtgtttcgt gacgcagaat tacaaggttt
     3241 tgcagatgga aagatgaatg aagagtttat tgaagatgtt agacatatgg atggaattaa
     3301 aaaaatatta cctctttatg ttatgaataa tgagatttct ggtaatggtg taacattatc
     3361 aagactagag ggtacagata atataaaact atataactct atgtttggaa tcaattatac
     3421 aaattttgag ataaaaaaac aagtaataga agcttttaat gataaacgtt ctgttatttt
     3481 gaatgaagat actctaaaaa aagtaggctt atctatagga gatacaataa ctctatctaa
     3541 tgataagtat gatttttctt ataaaatagt aggtagtttt aaatcccgcg caaatgatgt
     3601 agaagctgtt ataccatcac attatgcagt taatgatttt gataaaacta attatggttt
     3661 tttagtatat acagcagtca atccagatgc tgttatgatt cagataagat atttatttgg
     3721 agatacatat aattggagtc gtacagttga agaatttaat aatgattctt taaatactat
     3781 aagtagtttt ttgtctccta tgaataagat gacctatttc atttttcttc ttgctacagt
     3841 aggtattatt aataatctac tgattaatta tattcaaaaa agacgttcta tagctatgta
     3901 taaatctata ggactaagta ataaacaaaa tataaaagtg acattgattg aagggtttac
     3961 ttcaggttta cttggagctg ttattggtat tgtgatatct atacttgaga ttcaaactat
     4021 ctttatagtt gcaggaccaa aaatttcaat gaaaccagat ttagatttta aaacatttat
     4081 tattgttggg ttattgggaa ttatagtaac tttaataggt tcgatagtgc ctattataaa
     4141 agggaagaaa atgaaactga tagaagaaat taagtttgaa taattatgga ggttaatatg
     4201 aaaattacac ataatagttt agctgtagaa gcaaaaaata ttatcaaaga atataaaatt
     4261 ggaaatacaa caactcgagt gttaaaagag gtatctttac aagtaatgaa aggtgaattt
     4321 gtttctatca tgggtcaatc tggttctggc aaaagtactc ttttatatat tttaggtggt
     4381 ttagatactc caacgagcgg aaaagtgtat atgaatggag ctgatatatc acattttaat
     4441 gatgagaaaa tgagcataat acgtcgtaga aatataggat tcgtatttca attttataac
     4501 ttaataccaa atttaaatgt ggaggaaaat ataatgcttc ctttgttatt agatggtaaa
     4561 aatttaaaag attataaaaa tcaattagat gaaatcttag atatagttgg tttaactgat
     4621 agacgtaaac atacaccacg agagttatct ggtggtcagc aacaacgtgt tgctatagct
     4681 cgagctttaa ttggaaaacc agagattcta tttgctgatg agcctactgg aaatttagat
     4741 agtaaaacag ggatagaaat aatagattta ctgaataaaa taaacagaga caatggacaa
     4801 actataatta tggtaactca ctcaccagaa gcagccaaaa gtagtagtcg tactattaca
     4861 gtttcagatg gtttaattgt ataaggacta attattgtat ttgagataag catttttaat
     4921 tatataaatt agacatatgt aaagtggata taagttaaat actataaatg tgctataata
     4981 ttaaaataac agataaaagt actatatttt ttattgatta ttttaatttt atttgtttat
     5041 taaatatcta atgagtattc atactatctc tattattaaa agcagttaat gatatataga
     5101 gataatatgg attcttctta ctttaataaa tattcacgat aggacaggat ataatatatg
     5161 ttaaaaaaag aaagtattca ttttataaat aaaaataggt cacttatagt ttgtgaagac
     5221 aattttgttt atctcttgcc ttgtatacaa ttaagaaatt ggatatcgaa ttttactata
     5281 tcgtttccaa atcaaacaat aatttctgat aattacacga ttattcctca tggaagtgta
     5341 actttggtgt ttttttatga tgccacaggg ttacatagtc tattatttgg tccaactact
     5401 aaaccaaaaa cagtaggaaa tattgctaat agatgtgatg ttatactaat tatagaattt
     5461 caacctgctg gttttttccc gcttataggt atacagcaaa gtgaacttat tgataaagtt
     5521 gttccatttt ctataattaa tacttcatta gatttagaaa ttaaaaaaat atttaatgaa
     5581 tctttatcta tagataaatt gattttaaag ttggaagaat tattgatttc taatattaaa
     5641 attgaatact catatgagtt tattttggca attcagttga ttattcaaaa ctctggaaat
     5701 atatcttcac aagaaatttc taaaaaagta ttttatagtt ctaggcattt aaacagactc
     5761 tttaatcagt acctagggtt gagtatgaag tcattttctc gtttggtacg tattaataaa
     5821 tctataaaac ttctaaacaa taataaaact tcattgatgt ctatatgtaa tgaattagga
     5881 ttctatgata taccacattt tattaaagat tttaaaattg tctgtggaat aactcctcaa
     5941 aaataccgag ctaatatgtc tgatttttac agtgaaattg caaaatttta agttataata
     6001 cttataacaa agaaaggata gtgataagat gaaatttgga ggagtattgc taattgtcaa
     6061 agacataaat aaatcaaaaa agttttatga agatgttatg gagcaaaaaa tcacattaga
     6121 tttaggagaa catgtatcat ttgaaaatgg atttggatta caaagtaatt ataaagctat
     6181 aattggatat gatttaccaa tatatcaaca atcaaataac tttcagttgt attttgaggt
     6241 taatgacatc aaacaatggg aaagtaagat aaatagaata ggaaatattg aatttttgca
     6301 ttatataaaa gaatacccat ggggtcaaag aacttttagg ttttatgatt ttgataaaaa
     6361 tattatagaa atttcagaaa gtatggaaag cgtcataaag aggcttttaa aacaaggttt
     6421 agctttagaa gaaatttcaa aacgcactat gtatccagtt gaatttatta cacaatttca
     6481 acaattataa aatattaaaa tatataattt tttctttata ttgcaaaaaa caaaggcttg
     6541 gggcataata gaaccagaaa aacctgaatc tcaacagcaa atggctcata tggatttcgc
     6601 tgttaatgat ttagaagaag cagttcaata tgcaattcat tgtggagcta caatcgcaga
     6661 ggagcaattt actgatgact ggagagttat gattgaccca gctggacacc ctttttgctt
     6721 atgtcagatg aaatcaatta tggaaagtag ccatttttca ttgttataga ccaaaatatt
     6781 gtttattaat tacaaaaaag cacaacagta tataccgttg tgcttttact tattatacat
     6841 gaatacacta aaaaatataa atagtagagc tttatcaata atgataattt ttaaaaaata
     6901 gtattttttt attgaataat ttcttagata ttttaaag
//
LOCUS       DAMJWU010000145         6936 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMJWU010000145 DAMJWU010000000
VERSION     DAMJWU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.150"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            6..82
                     /locus_tag="PC460_003382"
     tRNA            6..82
                     /locus_tag="PC460_003382"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:His,seq:gtg)
     gene            91..166
                     /locus_tag="PC460_003383"
     tRNA            91..166
                     /locus_tag="PC460_003383"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Lys,seq:ttt)
     gene            174..247
                     /locus_tag="PC460_003384"
     tRNA            174..247
                     /locus_tag="PC460_003384"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Cys,seq:gca)
     gene            255..331
                     /locus_tag="PC460_003385"
     tRNA            255..331
                     /locus_tag="PC460_003385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Arg,seq:acg)
     gene            344..419
                     /locus_tag="PC460_003386"
     tRNA            344..419
                     /locus_tag="PC460_003386"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Val,seq:tac)
     gene            495..908
                     /locus_tag="PC460_003387"
     CDS             495..908
                     /locus_tag="PC460_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602236.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1217..4648
                     /locus_tag="PC460_003388"
     CDS             1217..4648
                     /locus_tag="PC460_003388"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF2602237.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            4890..6818
                     /locus_tag="PC460_003389"
     CDS             4890..6818
                     /locus_tag="PC460_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2602238.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         2868 a          771 c         1327 g         1970 t
ORIGIN      
        1 ttatggtggg tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt
       61 tcgactctca ttatccaccc caacttatat ggtctattag ctcagtcggt agagcacctg
      121 acttttaatc agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggcggcgaca
      181 tagccaagtg gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc
      241 gcctccaata aaatgcgctc atagctcaac tggatagagt gtctgactac gaatcagaag
      301 gttaggggtt cgagtccctt tgggcgcacc atatgaatag cgagggatta tagctcagct
      361 gggagagcac ctgccttaca agcagggggt cacaggttcg agccctgtta atcccaccag
      421 tgcaaacctc tagtaaaact actagaggtt ttatttttga ttgaaaaagt ttttatattt
      481 aggaggtaaa aaatttgtat atagtaatag gaaatgaaat agttgatagt gaagaactta
      541 aaataattat agataaaaat agtgaattta aagttgaaaa agatttaagt aaaagcacaa
      601 aaagagaaga tgtaatagca tatcagttaa gtatagattt aaattaccta gattctctta
      661 taaatgagca atgtaattta gccagtctat ctgatgaaga gaaatttgat gaatatatga
      721 ctttgtctga tgaacttgca ctggatttag aagagttaat gccaaaatat acaataataa
      781 atgctagagc atataaatta gacgaggtag atggaattgt aaaaataata ctagctgtag
      841 cgtatgcaga tttagggcac ttaaaacttt ctgatgtagt aaagaggcta tctagacaag
      901 tagattagga tatgaatatg aactatatta aaaaaatgta taaaatctta cgtaaagatg
      961 aaaataatta tgtaaacata gaaaaaaaca gtataaaatc aagtcaattg tagaaatttt
     1021 cacataaatt catctgaaat tctgtaaaaa atgccttaat tttaatgagg taaatcgagt
     1081 ttacacagtc taaaaattac ttatatttat aaatttatct tattaattaa ggaactttct
     1141 taaacatctt caaaaataaa gtattttagt ctataatctt taatattaca caaacaataa
     1201 aggagaagaa tagagtatgc ttaaaaagtt taataaaata ctagtggcta atagaggcga
     1261 aatagctata agaatattta gagcctgttc agaattgggg ataaaaagtg ttggtatata
     1321 cagtaaggaa gataagtatg gattgtttag aacaaaagcg gatgaatcat atttgatagg
     1381 agaaggtaaa gggccaatag atgcatattt agatatggat ggaataatag acctagcaaa
     1441 gagaaaaaaa gttgatgcta tacatccagg atatggtttt ctagcggaga atgcagaatt
     1501 tgctcgtaaa tgtgaagaga atggaattac ttttatagga ccatcatcta aggtaatgaa
     1561 tatgatgggg gataagatta actctaaaaa aatcgcaaaa gaagttaatg ttcaaactat
     1621 accaggagta gaaaaagcaa taagaagtac tgaagaagca aaggaagttg caaacaaaat
     1681 aggatatcct gttatgataa aagcatctaa tggcggtggc ggtagaggta tgagaattgt
     1741 acatcgcgaa gaagacttag agcttgaata tgaaactgct tgtagtgaat caagaaaagc
     1801 ttttggagaa gatattatat ttatagaaaa atatatagct gatccaaaac atatagaagt
     1861 tcaaatatta ggtgataatt atggaaatat agttcactta tatgaaagag attgttcagt
     1921 tcaaagaaga catcaaaaaa taatagaata tgcaccagct ttttcgttag atgataaagt
     1981 aagaaaagag atatgtgaag atgcagttaa gttatcaaaa catgtgggtt attctaatgc
     2041 gggaacatta gaatttttag tagatgcaaa tggtgggcat tattttatag agatgaacac
     2101 aagagtacag gttgaacata ctgtaacaga gatggttaca ggaatagata tagttcaaag
     2161 tcaaatacta atagcccaag gttacagctt agatagtgaa gaaattaata taaaatcaca
     2221 agatgatgtg gaaataagag gttactctat ccaatgtaga ataactacag aagaccctaa
     2281 gaataaattc atgcctgata caggtaaaat tcaagtttat agaactggtt caggatttgg
     2341 tataagacta gatggaggaa atggatttac aggggcaaac ataagtcctc attatgatag
     2401 tttattggtt aagacaatat cttgggatag aacattccaa ggagcaataa ataaaactat
     2461 aagatctata aaagaactta gagttagagg tgttaaaact aatgtaggat tcctagttaa
     2521 tgttttaaat aatcctatat tctcaaatgg taaatgtagt actaagttta tagatgaaaa
     2581 tccagattta tttgaaataa cagaaagtaa agacagagga acaaaattac ttcaatttat
     2641 aggagatgta attgtaaatg ataatgcttg taaagaaaaa ccattatttg atgcattaca
     2701 tgacccaaga atggataagg atggttcaaa atcagaagga agtaaaatac ttttcgataa
     2761 gttaggtaaa tctgcatata tagaaaagat aaaaaatgat aagaaattac tattaacaga
     2821 tacaacaatg agagatgcac atcaatcatt attagcaaca agaataagaa catatgactt
     2881 attaaaagct gctaagccaa cagaaaaata tcaaaaggat ttattctcac ttgaaatgtg
     2941 gggaggagct acttatgatg ttgcatatag atttttaaaa gaatctcctt ggcgtagatt
     3001 acaaaagcta agagaagaaa taccaagcat aatgttccaa atgttgctta gagcatcaaa
     3061 tggagttgga tataaaaact atccagataa tgtaatagaa gaatttacaa aagaatctgc
     3121 tagacaaggt atagatgtat ttagaatatt tgattcatta aactgggttg aaaatatgaa
     3181 accatctatt aatacagctt tagaaactgg aaaaattgtt gaagcaacta tgtgttatac
     3241 aggtgatata ctagataaga ctaaaactaa atacaatcta gaatattata ttaaaatggc
     3301 acaagaatta gaaagcttgg gagcagatat aatagcaata aaagatatgt caggtcttct
     3361 taaaccatat tcagcttaca cactagttaa agagttaaag aaaaatgtaa aagcaccaat
     3421 acatttacac actcatgata caagtggaaa tggtgtagca acttgtttaa tggcgtcaga
     3481 agctggagta gacataattg atgctgcatt agaatctatg gcaggactta caagtcagcc
     3541 atcacttaat gctatagtag aggcacttaa gaatacagag agagatacag gaatagattt
     3601 atttggatat gatgagttag gaaaatatta taaggattta agaaaagttt ataataaatt
     3661 tgaaagtgat ttaactaatt cttgtgcaga aatatataat tttgaaatac caggaggcca
     3721 atatacaaac ttaaaacctc aagctgatag tttaggatta gttaatagat ttgatgaagt
     3781 taaagaaaaa tataaagaag ctaatgaagt tgtaggagat ataataaaag taactccatc
     3841 ttcaaaagtt gtaggagact tagctatctt tatgactaaa aataaattag ataaagataa
     3901 tataatagaa gaaggtaaaa atttatcatt cccagattct gttgtggatt actgtaaagg
     3961 tatgattggt caaccagaag gtggaatacc aaaagattta caagaggtag ttctaaaagg
     4021 tgaagaagct ataacagtaa gaccaggttc attattacca gcagaagatt ttgatgaaat
     4081 agctaaatat ctaaatgaaa agtatgatat aaatgctaat ataagaaatg ttataagtta
     4141 tgctttatat ccaaaagtat atgaagatta tatcaaacat ctacaacatt ataatgatat
     4201 atctaaactt gaaagtgatg tattcttcta tggattaaat aaaaatgaag aatgtgaagt
     4261 agaaatagaa gaaggtaagg tattaacaat aagattagtt gaaattggtg aagttaagga
     4321 aaatggattt agaactatag gatttgagtt aaatggaatg gttagagaag tagaaataaa
     4381 agataagaat ttctctggaa aaattaataa tgttgaaaaa gctgatatga atgaccctct
     4441 tcaaattgga gcaagcatac caggaaaagt tataaaaata atggttaaag aagaagatga
     4501 agtaaaagca aatcaaccat taatcgttat agaggctatg aagatggaaa ctataatagt
     4561 tgctaagact gatggagtaa ttaagtcaat caaagtaaaa gaagatgata tggttgaaga
     4621 taaacaatta ttaatgataa tgaagtaatt taattaaaat aaattaaaaa gcactctgaa
     4681 atgtttaata aaaaatgttt tagagtgttt ttatttattt taattaaata tttgttaaaa
     4741 aaaagaggac aattacctcc agatgtagaa ataaagctag taaggaaaat gttatctaaa
     4801 aattctaaat atagtaaata ttttccataa tatttcttag gaaataacag caaagataaa
     4861 atacataaat ttaaaaggtg ggtgcttata tgaaggttta tgatagtaaa atgttgagaa
     4921 atgtagcggt attaggacat agcggatgtg gaaaaacaaa tctaatcgaa actatagctt
     4981 acacagcaaa cactaataag atacctaaat taacagacaa ggtaaatatg acttatagca
     5041 tgggattaat acctatagag tataatgatt ataagtttaa cttattggat accccaggat
     5101 attttgactt tagcggagac gttgtgtcat ctcttagagc aagtgatgca gcaataatag
     5161 taatagatgc aacagcgcct attcaagttg gtacagagaa atcattagaa ctaactgaga
     5221 gtattcctaa gataatgttt ataaataaaa tagataatga aaaggcaaga tacaaagatg
     5281 caatagcaat gttaagagaa aaatacaata ataagatagt tcctatgata agtcctatat
     5341 acaaagataa aaactttgta aagttacata atgtttttga aaacatagat gatttagaag
     5401 gtgaatttaa ggaacaagct atgagtgtta aagaagcatt aatggaactt atagcagaaa
     5461 ctgatgacca aatattagat aaatatttta atggagaaga actaactaca gaagagatac
     5521 aaaaaggtat tattataggt atacaaagag gtgatattat acctgtaata tgtggttcaa
     5581 ctataaataa tataggcaca aaggaaatat tagatacaat atcaagttat ttagaaccta
     5641 tctttacaga ggaaagcaaa ccatttaggg gattagtatt taaaactatg gttgacccat
     5701 ttgttggaaa gatgtcatat ataaaaatta ctgagggtgt actttctaag gataaagatg
     5761 tttttaatat aaataaaaat gtaaaagaaa aaatagctaa tatatataca ttgagaaata
     5821 gtgaattagt agaaatagaa aaagctaaag ctggtgatat agtagttata actaaagtta
     5881 attctctaaa aacaggagac actatatctg cagataaaga tgcagaagct ttagaaaaga
     5941 tagattttcc taaaccacaa atatattatg cagtaactcc taagaataaa ggtgatgaag
     6001 aaaaagtagc atctgtatta aataaactag tagaagagga ccctacatta cattggtata
     6061 gaaatacaga gacaaaacaa gcacttttag gaggtcaagg agaacttcat ataaaaacaa
     6121 ttaaaaataa aatgaaagat aagtttggtg ttgatgttga attgaatgac cttaaagttc
     6181 cttatagaga aactataaaa ggaacagctg atgtacaagg aaaacataaa aagcaatcag
     6241 gtggtcatgg tcaatatgga gacgtaaaaa taagatttga aagatgtgaa tctgattttg
     6301 aatttacaga agaaatattt ggtggttctg taccaaaaca atatatacca gcagttgaaa
     6361 aaggtttaaa agactctatg caaaaaggta tattagcagg atatcctgtt acaaacataa
     6421 aagctacgct ttatgatggt tcataccatg atgtagactc ttctgaaatg gcctttaaga
     6481 tggcagcaag tgcagccttt aaaaaaggta tggaagaagc acacccaata ctactagaac
     6541 ctataatgaa acttaagata actgttccag aagaatatat gggagatgtc atgggtgata
     6601 taaataagag aagaggaaaa atatttggaa tggaaccaga tgacaaaggg aaacaaataa
     6661 tatttgcaga agctccacaa gcagagacat ttaaatatgc aatagattta agagcaatga
     6721 cacaaggtag aggatacttt gaaatggaat tagagagata tggagaggtt ccatcacagt
     6781 ttgcagaaaa aataattggg ttagctactg caaagtaatg aacaaaataa atagaaaaat
     6841 aattaatata tattattaaa aatattttta gggttttaac taggtatagg tttatgttag
     6901 gtaaacttat acctagtttt tatacatttt tgatga
//
LOCUS       DAMJWU010000146         6918 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMJWU010000146 DAMJWU010000000
VERSION     DAMJWU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.151"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            63..1319
                     /locus_tag="PC460_003390"
     CDS             63..1319
                     /locus_tag="PC460_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2602239.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            1342..3423
                     /locus_tag="PC460_003391"
     CDS             1342..3423
                     /locus_tag="PC460_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF2602240.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSMKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            3460..4545
                     /locus_tag="PC460_003392"
     CDS             3460..4545
                     /locus_tag="PC460_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2602241.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGFVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            4599..5723
                     /gene="wecB"
                     /locus_tag="PC460_003393"
     CDS             4599..5723
                     /gene="wecB"
                     /locus_tag="PC460_003393"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF2602242.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGMQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            5801..6775
                     /locus_tag="PC460_003394"
     CDS             5801..6775
                     /locus_tag="PC460_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2602243.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         2886 a          647 c         1145 g         2240 t
ORIGIN      
        1 aatttttaaa ataaatgatt ttatattaga atcaatattt taaaatattt taggagaaat
       61 ttatgagaga tttaaatgta tcagagtatt tgttcgtatt tagtctgttt agtatatggt
      121 ctttattgct tataaatatt attttagcta tgggtggata tatattttat tttaaaaact
      181 ttgataaaga aataaaagaa attgatgaat atcctatgat atcaatactt gttcctgctc
      241 ataatgaagc taaagtgata ggtagaactg tggagtcttt gctattactc aattatccta
      301 agagtaagat ggaacttatt gttataaatg acaattcttc tgataatagt aaagaaattt
      361 tagaaaatat aaaggatagg tacaacaatt ataatttcac tataataaat acagatagtt
      421 taacaggtgg aaaaggaaag tctaatgcat taaatattgg atatacgatt tcaaaaggag
      481 attttatagc tgtttatgat gcagataaca caccagataa aaatgcactt agatatctag
      541 tacaaactat agtaatgaat gatgaattag gtgctgttat tgggaaattt aggactcgaa
      601 ataaaaataa aaacctatta accaaattta taaacataga aacattaagc tttcaatgga
      661 tgtcacaagc tggtagatgg caacttttta atctatgtac aataccagga actaacttta
      721 tattgaggag aagtatcata gaagaaattg gaggatggga tagtaaagca attgcagaag
      781 atacagaaat aagttttagg atatataaat tgggatacaa gataaagctt gtaccacaat
      841 ctataacatg ggaacaagag ccagaaactg ttaaggtgtg gataaagcaa cgtactagat
      901 gggcaaaagg gaatatatat gtattaatga aatatataaa aaatatattt aaacagggaa
      961 gaaataagat tgtgtttgat atagcttact ttttttctgt atattttcta tttttaacat
     1021 ctgtaattat atccgatatt ttatttgttt taagtatatc taaactagta gaaatttcta
     1081 ttccaattaa cttttttcta atatggatac tatcttatct tttatttatt atagaagtga
     1141 gtataagcct tactatagaa aaaggtgaag caacaattga aaatatattc atagtggcaa
     1201 taatgtattt tacatactct caactatggc tgtttgtagc tataaaagga atgatagagt
     1261 atctaaaaga tataattttt aaaagagagg ttaaatggta taaaacagaa agattttgat
     1321 tcatgtacag gaggaattaa catgaaaaaa tttattatat caattatatc tttagtattg
     1381 tttttttcaa atatatcact tatatacaaa gtaaatgctg atgagactaa agttaaaaat
     1441 tataagtttg aaagagatat aactattgat ggtgttatag gaagtaatag tacttttttt
     1501 gaagtaaata aaaattggga tattgaagaa gttttattgc atcttaattt tagtaagagc
     1561 caaatattaa atggagatgt ttcaagttta actgtattga taaataatgt accaataaaa
     1621 tctattaaat taaatgctaa gacaaattat aaaaatacat tagaagtatt agttccaaaa
     1681 gattatataa ttcaaggata taatgaaata aaaataaaaa catacaaaac aatatctgat
     1741 aaaatttgcc aagatgattc caatactggg aactggatgg ttattcacaa agaatcatat
     1801 atatcaatta gatataagca gaaaaaggtg gaaaactcaa taaatgaata tccgtatcca
     1861 tatgcagaaa ttgaaaataa tcataaatta gatacaacta tagtagtacc agataatatg
     1921 actagaggag agactacagc agtgtttaat ttggcatcag catttggaaa aataacaaaa
     1981 aatgatgatt taaagttaga tgttaaatta tattcagaaa tgaaaaattg gtcagatgat
     2041 aatattatat atattggtaa acctgaaaat acagcagaag aaattttgga tattttaagt
     2101 ataaaggaac aaaccctatt atcttcaaac tgtataatca agcaagtaga ttcaccatac
     2161 aataagaata agaagatgat ggtggtaatt ggtagtaatg aagatgattt aataaaggca
     2221 tcaaatttat taatagaaaa tagactttct aaccaagttt tatcatcatc agtacttgta
     2281 aacaaagaga ctaatataaa aattaatagg gaacaaaaat taaatttagg tcatttaaca
     2341 ttaaaagact taggatattc tgactttttg ttggaaggag catttaatca acaagctttg
     2401 tttgatgtca aaataccaac gggtaaggta ttagatgatg gttcaaagat tatcttaaat
     2461 ttaagatatt ctgacaattt agattttgaa aaatctttag ttacagtgtc tataaatgat
     2521 gttattgtgg gaagtaaaaa attggatagg tcacattcta ataatgataa gttagaatta
     2581 aaaataccta aagatataga taataaaaac tattatcaag ttaaattgac atttaatttg
     2641 agtataaaaa attcgaattg tgttactaga gagagtaata atccttgggc atatgtatca
     2701 aataattctt atttggcgtt atctatgaaa gaaaatgaaa ccttatcttt tgaaaattat
     2761 ccgtatccat ttgttagaga tgatgaattt aatgatttaa ctgtgataat gccagattat
     2821 tcagggtcac aagctatgac ttggatgttt agattaggtg ttactttagg tgcgaatata
     2881 aattcacata atggaaatat aaatgtaatt agaggaaaag aatttagtga caagtataag
     2941 gatactaata ttgtagtttt tggagtgcca cataataatt ctgttataaa gatgttaaat
     3001 aataatttaa atataaaatt cgataaaaat tactccaatt ttatatcaaa tgataagata
     3061 agctttattg atgattatgg aaaaaatatt tctaccattc aattaataaa atcaccatat
     3121 aataatcaaa aaaatatcat ggtaattagt tcaatgaatg aaaaaaactt atatttagga
     3181 atggattatt tgttgaataa aagtaaagtt aatgatttaa aaggagatac attgataatt
     3241 gatgaatacg gagaggtaga agatttagct tataatttaa aatctaagaa agaagtaaaa
     3301 gattcaagtt ggaatatgag tataaataag acaactaaag tatttttaat gatttctttt
     3361 attactataa ttgtagtcat gatattatca atgctatata taaaaaaata taaaagaaga
     3421 taatatgata aatttattat tttaaaagag gtatataatt tgaggaaata tatgaataaa
     3481 tttaaattaa aaagaagtat tttaatctca ttaataatct taatttttat atttatagtt
     3541 tactttgtaa gtattgagtt aaaagatagg agtctagtaa ataatcaata taaaggtaag
     3601 attaaatcgg ccaatttatc tgtagattat gaaataaatc aagttatgaa ggatatagat
     3661 aaattgggat tgaatacagt aaatgtacct ataattataa atgtagagtc cattaattct
     3721 gatattatgt caatagataa taatagtaaa gaaaaggcaa ttaagttaat aaaaaaatta
     3781 aataaaaaag gtatttcaac aatattagag gcatatccat ggattgaaaa tggaaaattg
     3841 tatgagactg actggaaccc tataaataaa aaaagatttt tttatacttg gcaaaatgcc
     3901 atattaaatg aacttataat tgatgtagca aatccactgg atgtaaatgt tttaaatatt
     3961 ggttctaatt ttgtacattt ggaagaatat caacaaaatt ggggtgaaat aatagatttt
     4021 gtacaatcca aatttgacgg ttttgtaacg tacagaacta attggtggta tacaaaaaaa
     4081 gatgatttaa gtagtaagtt gttttatgaa caaaagctga acaataattt ttttgataag
     4141 ttagatttta tatctatagc agcatatttt gagttaagta ataaacctgt taatacagtg
     4201 gatgaattaa tttctgcctt acactcatct actgtaaaca atagaggaca aaatataaaa
     4261 caagaaatat ataaccttta taaagctcat agaaaaccta tattttttgg agaattaggt
     4321 ttttcaaata gagaaagtgc aagttctcag ccgtggaatc atactccttc aaaagctgta
     4381 aatggcgaag aacaagcaag gtgttttgag gcatataaga aagtttttga aaatgaagac
     4441 tggataaatg gtttttctgt gttttgtgta ggtaaaatag atgacgaaaa aaatttttac
     4501 ccatcaaaag agagtataaa agttattaag tcttggtatg aataattttg aaagtgtaga
     4561 atttatatag attaaataga tgattaggag agacataaat gaatgacatc aaggttatga
     4621 ctgtatttgg aactagacct gaagctatca aagtagcacc tttaataaag gaacttgaaa
     4681 aaagagagaa tataaaaagt attgtttgcg ttacagctca acatagagaa atgcttgacc
     4741 aagttataga aacctttaat ataaatgtag actatgattt ggatataatg gaaaaaggac
     4801 aaagcctgaa tgatataaca tgtaagattt taaataaact tcccttgatt ttaaacaagg
     4861 aaaatccaaa tataatattg gtacatggag atacaaccac aactctagct acaagtttga
     4921 ctgcatttta taataaaact cttgttggtc atatagaagc tgggcttaga acctatgata
     4981 aatattctcc ttttccagag gagttaaata gacaacttac aggtattatt gctgatatgc
     5041 actttgctcc tactaatttg gctagaaaaa acttgatttc tgaaggaaaa cctaataata
     5101 atatatttgt aactggtaat actgctattg atgctttaaa gatgacaata aaagaaaact
     5161 ataatcatcc tataattgat gagattggta acgataggat gattttacta acctctcaca
     5221 gaagagaaaa tttgggtaaa cctatgaaaa atatatttag agctataaag cgaatagtag
     5281 atgactttga agatgtacag atagtatatc ccatacattt aaatcctaaa atacgaacta
     5341 tagctgatga gatatttgga aaatttcctg aaaaaattca tattatagag cctttagatg
     5401 tagctgattt tcataacttt ttaaataaat cttatatgat aatgactgat agtggaggaa
     5461 tgcaggaaga agctccatca ttaggtaagc cagtccttgt gttgcgtgat aaaacagaaa
     5521 gaacagaagg aattgaagca aagacactta aattagtagg tactaatgaa gatagaatat
     5581 ataattcagt gagcgattta ttaataaata aagataacta tgttcagatg agtaaagcat
     5641 ctaatccata tggagatgga aatgcaagca aatatatagt ggatataata attaaaaaat
     5701 ttaattgcaa atatttaaat tagataagac ctaaaacatt aactaattga tataagattt
     5761 atgataatat taaggaatac aatttttaag ggggagtaat atggctagaa aattaataaa
     5821 aaatttgggt aaaagtaaaa gtgtaaagag agtgaaactt ttatttaaga agatttttat
     5881 tactgtattc atagtagcaa gtatagttgc tatttttaat ataacgaaat attttgagga
     5941 attatacaaa gtaagggatt taaaaagtac taaaattgaa tactatatgg atgtggctga
     6001 tgaagcagga gatggaaagg tccaattaag ttggaaggcc ttacttgcta tagacatggt
     6061 aatacatgat gaagatttaa gtaatataaa aaagaaagac acattggata taggggaaaa
     6121 gtttatagta gaagataaaa atgataaagg cgaaaaggtg tataaggtaa aaaagtttaa
     6181 taaggtatta agcgaattga aatttgactc ttctcaaaaa agtagagcaa gaaaatacat
     6241 gaaagattta gaatacacat accttggaaa taaacaatta gatagtagtg atgaaaaaat
     6301 taaatttata aagaagttag aagactcagc tataagagaa tatattgatt atggaatatt
     6361 gccctctata acaattggac aagctatatt agaatctggt tggggaaatt ctaaacttac
     6421 aaaacagagt aataatttat ttggtataaa agcagataaa gcatggaaag gaaaaagtgt
     6481 agaaatttca acttcagagc attataatga aaaaattgta gctagtttta ggtcgtataa
     6541 ttcattacaa gattctgtca aagaccacag tttattttta attaataata aaagatatag
     6601 aaaacatgga ttgtttgagg caaaagatta tattagtcaa gctcaatcat tagaaaatgc
     6661 aggatatagt acagcagaag ataaaaaagg aaatcgtata tatgcagagc tattaataga
     6721 tgttattagg agttataatt tgcaattgat agataataaa gttgagacaa aataattgtt
     6781 aactttatat gaggagataa caagataaga ttcttttcta caaaaagaat agaaaaattg
     6841 taaaaaaaac taaaaaaatg tattgacttg ttatttaaaa ggtgatatta ttattactgt
     6901 actcgaaaga gaacaaaa
//
LOCUS       DAMJWU010000147         6803 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMJWU010000147 DAMJWU010000000
VERSION     DAMJWU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.152"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(<1..138)
                     /locus_tag="PC460_003395"
     CDS             complement(<1..138)
                     /locus_tag="PC460_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2602244.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KN"
     gene            complement(786..1193)
                     /locus_tag="PC460_003396"
     CDS             complement(786..1193)
                     /locus_tag="PC460_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602245.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1667..2551)
                     /locus_tag="PC460_003397"
     CDS             complement(1667..2551)
                     /locus_tag="PC460_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2602246.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2842..3318)
                     /locus_tag="PC460_003398"
     CDS             complement(2842..3318)
                     /locus_tag="PC460_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF2602247.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3584..4759
                     /locus_tag="PC460_003399"
     CDS             3584..4759
                     /locus_tag="PC460_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF2602248.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4840..5643)
                     /gene="proC"
                     /locus_tag="PC460_003400"
     CDS             complement(4840..5643)
                     /gene="proC"
                     /locus_tag="PC460_003400"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2602249.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5683..6267)
                     /locus_tag="PC460_003401"
     CDS             complement(5683..6267)
                     /locus_tag="PC460_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602250.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2240 a         1145 c          757 g         2661 t
ORIGIN      
        1 gtttttataa acatctattc ttttagcaag atacttatta tatacaaatc tacaacatcc
       61 aaaagttttg ttaattaact cttgttgttt tttatttgga tacattctaa acttataagc
      121 ttttttcact gctatcacct cactttaatt gttttttgat acacttttta atgattttat
      181 cacattttat cacatttaat atattatata atgctgtggt aaacaattta ttaatcacct
      241 ataataatgt taacaaatgt aattatttaa aacaattatt tagaaggttt tatatttaat
      301 tttaaataac atatttaaga accgtaagtc ttaactttta tcattttgag gttgtcgttc
      361 acataagttc gctactacct atgcagttct cttatgaact tcttacattt tcatgtaagc
      421 acagactata tcttatcctt cagcattatc cgttaaggtc tacccacttc cactaccaat
      481 agcttgtagt gtacttccct caagaggaat agtcgttgaa gtttctcctg ttcggagttt
      541 acctgctgat tgcccatttt aaagtactta ggatttaacc ttataccatc taaccaattt
      601 tttctacttt cgtaactttc acacttaggt ttatttcatc cttatgtttt agtttgatta
      661 gctttagggg tttccagcaa ttcaagtagt attggatagg ttctatccta tctctacata
      721 taggtttccc tatatgctga ctattttagc tactaactca tgactaaagt cacgagtgtg
      781 tgtagctatt ttttaatcaa tattttagta aattttacgg taagtaaaaa cctctctcct
      841 tcaaatccta tagcaaatat acttacaagt tgctttgaga aaataactcc taatatagct
      901 acaactatgc atataccaac tattatattt aaaacattgt ttaaaaactt tagtgcttgc
      961 ttttcacctt gtttactact tatatcttga tacattggaa taaaagtagt aactatagca
     1021 gtacctattg ctgcaaatat aatatttggt atattcatag ctgttaaata actctctgta
     1081 tataagcctg taccataaat tgatgataat actaattctc tacccattcc aagaatctta
     1141 gagattatag taactatcat caaataaaaa gttgctttag ctaccttgct cattttatca
     1201 ctccacttct aacatacctc tttttaatca agtaaaattt agctcattag atataattct
     1261 atcaactaat ttgccttgct tagccctatc aaaatataat taacacctat ttcttaatat
     1321 atcacaaact acattattct tcatattttt ttatttctcc aataagtttt acatttttcc
     1381 tatataaatt gtatagcttc aattatattc aaagaaatgg agttgctacc cgataacttt
     1441 aaaagtaaaa aacagatttt attatctttt taaactatat tctaacatat tttgtccata
     1501 ttgagaaatt ttactaaact attatgtact ttattaaata aataaaataa cagcacacct
     1561 ataaaataca agtgtgctgt tcataaacag attttataaa aaaataattc ctaagtagag
     1621 attaacatgc tttctcatac tctcatatat aatgtgtcta aaaatattaa taaaaatgtt
     1681 ttaattgttt taacttactt ggatgtctta attttcttat tgctttagct tctatctgtc
     1741 ttattcgttc tctggtaact ccaaatacct ttccaatctg ttctagtgtt ttagggtcat
     1801 catcatcaat tccaaatctc attcttaaaa cttgctgttc ttgctctcca agacctgtaa
     1861 gtagttcttc tatcttttct tttaaattat gctgttcaac ttcgtgtatt acaacatctt
     1921 taaaatcagc atctgatggt ataaattcaa ctaaactact atcctcatct tcttttaatg
     1981 gagtatccaa agatacaaca tttttatctc ttcttaataa ttccaacact ttgtcaactt
     2041 ctaagccaca tgcatctgca atctcatcta tagttggctc tcttaataat acatttaaaa
     2101 gttcttgttt ctttttcttt acaaaattta ttctctgatg aagatgtata ggtatcctaa
     2161 tagtattttc gcaatcgtcg atatatcttg ttatactttg ttttatccac caagttgcat
     2221 aagtgctaaa tttgtatcct tttttataat cgtacttttc tacagctttt ataagtccta
     2281 tatttcctgc ttgaattaaa tccaacatat caatactatc tcttttatat ctctttgcta
     2341 tgctcactac caacctataa tttgaattta taaatttttc ttttgctact gaagatgagt
     2401 tgattatttc cttagccagc tctacctctt ctcctgctga caacattttg tattcctcta
     2461 tttcctttaa atacattttc atcgcattcg atacatttga tgaacccaca caaatataat
     2521 tttcataatt attttctttt ttagtatcca taatcaaacc ccttctattc atagaatttg
     2581 ctaaaacaaa aataaataat ttaaacctac ataatctaca attacataat atcccattct
     2641 ttaaatgaat tgttttgtat attatgtata caaatttata aaaactatac ctataatatt
     2701 attctacttc taacagcaat atcctcctta tatatataat ttattttatt atatgttgtt
     2761 atattcttag ttttttgaat gatatatgtc taaaaaaaga ctgtttacta aacagtcttt
     2821 ttttatatat atgctcaata tttaattttc atgatgatga ttacatccac atccacaatt
     2881 ttcatcatgg tgatgatgtt cttctaaatc ttttacctgt tgttccattt catgaaactc
     2941 ttttaccatt atatcataag ttggaagagc tgcctcagta tcgtattctt cccctctcca
     3001 atcagcgtac atcatagcat ctccattagt taaaaccaat ctacctgaca tagttatatt
     3061 atccacttct tctaaaaatt ctatttgttt ttgtaaaact tctccttcta actccacatc
     3121 ttcatctaaa cattgcacta tcattacagg tgcataataa tcttcttcat ctttaacatt
     3181 tacaacaata tctaaaattc cttctgcacc ttcttcaaat tctgccaact caacatttgt
     3241 atcaatcata tcttgaggta ctaataaatc ttctattaaa tattttatta ctctatcttt
     3301 tactaattct tttgacattt atttttctcc ttccgattct ataacattat cataatcatt
     3361 tctatattat agtatactac attaattgtt ttttcaatct cttacaaaaa ttatttttta
     3421 ttatcttcct tatattccct tttttattac aaagttgtaa tcgcattgat ttaaaaaata
     3481 gtttatattt agtataatag aaatgtagca catattttta ttcaatatat taataaaaaa
     3541 atatattgaa aatgtaatgt atgttaaatc taggaggaat attatgaagt ttaaaaaaca
     3601 aattagttct attgttgtgt ctactatgct tgtacttggg agtatgaacc tttcttatgc
     3661 agacggtaca aatgtagtta cacttggagc taacttatca gagtctcaaa agcagcaaat
     3721 gcttaattac tttggagtaa aaaaagacca agtcttagtt ttagatgtaa caaatgcaga
     3781 agaaagacag tatttacaag gtgttgcaac agagggtcaa cttggaaaag ttactatctc
     3841 ttgctcatac gttgaaccta ctaaaaaagg aaatggaatt aatgttaaaa cagcaaactt
     3901 aacttgggta actagttcaa tgattgcttc aacccttaca acagctggtc ttgaagatgc
     3961 aaatgttatt gctgctgctc catatcctgt aagtggtact ggagcattga caggaatcat
     4021 gaaagcattt gaggatgctt caggaaagaa acttgatgaa actaagaaag aaattgcttc
     4081 tgaagaattg gtagttacag gtgacttagg agatgatata gggcaagaaa aagcaacagg
     4141 tgtaatgaat gatataaaaa cagaaattgt aaaaaatggt acaaaagata ccaatcaaat
     4201 agcaaataca ataaataatg ttgtaaataa ttacaatatt actattacac cagaacaaaa
     4261 agaacaaatt aaaggtgtaa tgaaaaaaat agctgaccaa aattacgatt acaatagcat
     4321 gaaaaataca ctagataatg tatcagataa tgtaaatgaa caattaaaga aattaggtga
     4381 aagtgttaag ggttctggta tactagatac tattggtgga tggtttagtg gaataggaga
     4441 ctggttctct ggtttattct caggcggaga taaaaaagat ttgggaatct taaataatac
     4501 taatgatgag tctcttggct ctgatgctgt aataaattct acagaaggtg ttcaaataaa
     4561 tccacaaaat aatactaatt ctaacaatga tagtgataca aatagctcaa aaaatgatac
     4621 ttcaagtaat aatgaaaata acgatagttc tcaaacaaat gaaaacaaca caacaaataa
     4681 ttcagaaaat caatcaaata ataatgactc agttggtttc tttgaaaaaa taaaaaattg
     4741 gctttcaggc ttattttaga aattaaacaa ataaaaagaa ttgcctaaaa tggaaatttt
     4801 ctataaaagg caattctctt tatttacaat aaatttttat tactcactca tttttttaga
     4861 tttttctata catgcacata ttgcttttat cacagaagct ctaaaacctt cttcttcaag
     4921 tactttaact gcttctatag tagttccacc aggtgaacat accatatctt ttaattcacc
     4981 aggatgtttt cctgtctcaa gtaccatttt tgcagaaccc ataacagcct gtgatgcaaa
     5041 cttatatgct tgtggtcttg gcattcctgc aattactgct gcatctgcta tagcttctat
     5101 aaataaaaat acatatgctg gtgcagaacc acttgcacca attacagctt ctataagata
     5161 ctctggtact acctcagtat ttccaaaaga attaaatact tctgtaactg cttctaagtc
     5221 ttctttgttt atatttttat ttatagaaag agaagacatg gcttcattta caagagctgg
     5281 agtatttggc atagttctaa ctattttttt atcttctcct ataatagact ctatatcttt
     5341 tatagatttt cctgctgcta ttgtaactat aattttattg tcatctataa agtcttttat
     5401 ctcttccaaa atatcatcat atatatctgg tttaacagct acaattacta tatcagaggt
     5461 tctagttact tccttcgagt cttttgtagt atttattcca aaatttgcat ctattctatc
     5521 aagagttcct tgactatatg ctgatgctat aaccatatta ggttcaacta attttgaatt
     5581 tactattccc cctatcatag cgcttgccat atttcctgca cctatgaatc caattttttt
     5641 cactttagta cctccttatt gactaatagt aatcgttaaa atttaagctt ctttaactgc
     5701 atcagacttt tcaaatggat ttggtatact tactaccaaa attttcacta ccttattagt
     5761 atagttgacc caattatgat tgttcttttc actattatag tgtattgtgt ctcctggata
     5821 catttctatt tgttcatctc ccaaaaacac tgtaagagta ccctctaata catacacaaa
     5881 ttcctcacct tcatgtacgt aacaacaaat ttcctcacta ctcttacttg gaagaatttc
     5941 aattaatctt ggtagcattg ttttttcctt taaactagat gataaatgat aatgtataaa
     6001 tgttgagttt tctacatcaa atacttcttt ttcataactt cttaatacag ctcttttatg
     6061 ctcttttggc ttcataaaaa agtaagttaa ttctacatct agtacacttg ctatttttcc
     6121 taaggaatct gttgccacac ttgttaatcc tctttcaagt tgtgataaaa aaccaattga
     6181 caagtttgtt ttttcactca tatccttcag cgtcatttgc ttttgagttc tgagttgctt
     6241 tatttttgct cctatgtcct tattcaaaac catcacccct aaataatttt aattttttca
     6301 taatataaat acaaaaatat aaatataggt actgaaccta tctttatttc atttaaaaaa
     6361 atttttataa gtattactat aatttatcaa tctttttttg tctacatttt tacgattatt
     6421 tacgatttat ttttcacata tgtatattta atcttgccaa ctaaagatac attttttata
     6481 agcatttact ctttatataa taaaacttaa gttgttcatg agtcttcttc tggtttaatt
     6541 attaaaattt caaattatat tattatttct acacattatt acatccttta gtacaattat
     6601 aacataattt tacaatttaa tgatatctat tcaatgtctt tttttactct acaacatatt
     6661 ttattttaga gtttaatact atatatattt tttatataca gccttgtcat tttttagatt
     6721 gtcaagagtt attgaagtat tttatgtaga tttagatttt attttctaat ttctcattta
     6781 acaagaattg cttatgataa aaa
//
LOCUS       DAMJWU010000148         6781 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMJWU010000148 DAMJWU010000000
VERSION     DAMJWU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.153"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..107
                     /locus_tag="PC460_003402"
     CDS             <1..107
                     /locus_tag="PC460_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2602251.1"
                     /translation="EWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            234..1136
                     /locus_tag="PC460_003403"
     CDS             234..1136
                     /locus_tag="PC460_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2602252.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1748..2761
                     /locus_tag="PC460_003404"
     CDS             1748..2761
                     /locus_tag="PC460_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2602253.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPSDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            3117..3686
                     /locus_tag="PC460_003405"
     CDS             3117..3686
                     /locus_tag="PC460_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2602254.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYFNRYN"
     gene            complement(4022..5359)
                     /locus_tag="PC460_003406"
     CDS             complement(4022..5359)
                     /locus_tag="PC460_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2602255.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYTLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMIFYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(5803..6513)
                     /locus_tag="PC460_003407"
     CDS             complement(5803..6513)
                     /locus_tag="PC460_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2602256.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT         2517 a          715 c         1047 g         2502 t
ORIGIN      
        1 gagaatggat ttgtcctagt tgtaatgaaa ctcacgatag agatataaat gcaagtataa
       61 atatattaaa agaaggatta agactaataa caattcaaaa taaataactt atgtaagaac
      121 cgtaggaact acggggatag cctgtggaga attagtaaga catatgttag tatgcaaaat
      181 tctatgaagc aggaaccctg tgactttagt catgggaggt tcagattgga aatgtgttgc
      241 cacaagatta ttatggatta atagtgattt taggtgttat tcttggagtt atgggggcat
      301 tttacaactg gtttactcta tatgtgcagt ctttatacaa gaagattcct aaaattggta
      361 cgtttggaaa actgttgatt ccatttataa tggctggtat tcttgggctt gttatgcctg
      421 atgttctggg gagtggacac aatttagttg aagaacttac aagtcatgaa tatatttttg
      481 gaatggttgt tttgattttt gttatacgtt ttatattttc ggctgttagt tttgggtcag
      541 gtgcaccagg gggaatattt tttccattgc ttgttttagg agcatttata ggtggaatat
      601 ttggtatggt aggtgttaaa gtttttggaa tgaatcccga ttatgtaaat aactttgtgc
      661 ttttggctat ggcaggatat tttacagcta ttgtgcgtgc ccctttgact ggtatcatat
      721 taatttttga aatgacaggt tctgttagtc aaatgctttc actatcagta atttcaattg
      781 tggcatatat tgtggctaca cttatgaagt ctaaacctat ttatgaaagt cttcttgaga
      841 ggttacttga aaaaaatgga gaacctgtac ataaagagag gggcgaaaag atattagacc
      901 agttctctgt tatgtttggc tctcctgttg cgaacaaggc aattcaggat gtacagtggc
      961 ctgaaaattg tcttttggta gcaattcaaa gaggtggaga agaaattatt cctagaggta
     1021 agacaattct tagaccaagt gatgtaattg tgactatgac agatgagaga gatagtggtg
     1081 ctgtgtatga taagatggaa aatttgtgta aagaacatac gtcacatatt ttttagggat
     1141 aatgatgaat gtttaataat gatagaatat ttaatgaaga tttgattaaa aaattggtaa
     1201 tttgaaagag agttaaggaa ttaatataag aaaatgaaaa tgtgatatct tagatgtaaa
     1261 agagtttgaa gaaaataatt gtatatattt gttaatgaga ttatatggac taatggtata
     1321 tttatactgt gctaaaagtt gcactattgt atgaggaatg ggaataaagg gtttgaaaaa
     1381 ataggtagaa gatttttcag atattcttac tgggatattt taattaatat attttaattg
     1441 cgatattttg ggaagaatag aatttattta taaatgaaaa taattaaaag gggttaaggt
     1501 tgtattagcc ccttttgctt atagagtttg gaaatggtat cttgatagca gttgtttcat
     1561 gcttattata ttttattaca tattttaaaa ttgaccttat tactcctaaa tcattgttat
     1621 atcaactttt aaaacacttt caagacaatc gcctattgaa aacaaagtta attaaaaacc
     1681 tacatatcag tttagtattc gtgtagatga ttatataaat ataaaagtat aagaaaggtg
     1741 agtatctgtg gttagttcat ttttaaatga tatctttaga acaattttag taagttgtat
     1801 ttttatagtt attttattag tctttagaat aactttattt aagattttca gtaaaaaatt
     1861 taattactat atatggctta ttgtgattat taagctatca ttaccattta tgaattatac
     1921 ttttatattt aatgagtcaa aatataatgg taatattaat aagataagtt taggcaattt
     1981 taatagtatt tctattgtat atagtactgt tttagtctct gtttggattg ctattacgat
     2041 attttattta gtttatactt tattgaaata tattaagctt aaaaatatga taaatgattt
     2101 gtcttatgat gttgaggatg ataagataaa aaatctttat gaaaatttat taaaagagct
     2161 taagataaat aaaaaaatac ggcttaaata ttcatatgaa gttgaaagtc cagtattttt
     2221 taattcgtgt gtcattttac caccaagtga ttatacatta aaagagttaa actggatatt
     2281 taggcatgag ttgatgcatt ttaagagtaa agacctttat attaagtatt tggtgatatt
     2341 tttaaaatgt gtttattggt ttaatccttt tgtgtatata atggagaagt ttatagattt
     2401 ggattgtgaa ttttattgtg atgaaagagt tttgaagaat tgtacgatag aagagaagca
     2461 ggattatgca ttgacaataa ttagtgcaat gagaaaaggt tcaaatagtt caaataaatt
     2521 tgtggctgga ttacataagg agtcagatat taaaaaaaga gtgtttagta tgtttaatga
     2581 aaagtacaga aatggtattt tgatagcagt tattttatgt ttattatctt ctattacata
     2641 ttttaaaatt gatttaatta ctactaaatc aatattttat ccactttcaa aacaaacacc
     2701 tattgaaaac agaactaatt caaaacctac atatcagatt aatgtttata tagataatta
     2761 aaaaatacaa agtttaacaa gagattaata taaataattt taataagtat ctttcacata
     2821 tgttaaattt actttaatta tattaaagcc aatgaattat tctttagaag ctccaatgcc
     2881 agtaaataca ggtaaagaaa aatattttac tacaattgca gattaaaaag tgttctaata
     2941 tttaaagaaa tacacaagtg taagaagggt aaaaatctgt tataatacat taaaaactaa
     3001 atttagaaaa atttacataa ttagaaaaat gatttgataa aaaataaagt taaatattaa
     3061 ctaagtaaat ttgaaaaaga aaaaatataa agtacaaatt taaaaggagg taactaatga
     3121 aaaagaagtt ggaagatatg actttagagg agttatggaa cttgtttcct atatttttga
     3181 ctactcacaa agaatgttgg aaaaattggt ttaaagaaga acaatataga ctaactggaa
     3241 tattgccaat gcaaaaaatt gttagaataa gtcatattgg aagtacttct gtgagtacga
     3301 tttatgccaa gcccattgtt gacatactga ttgagataat tcctgattat aatatgaaag
     3361 atattaaaga tttgattgag tcgaatggat atatttgtat gtctgagagc agtagtaggc
     3421 tttcacttaa taaagggtat acagtagatg ggtttgaaga acgagttttt catttgcata
     3481 taaggtatgt aggtgataat gatgagttgt tttttagaga ttacttaatt gaccacccag
     3541 atgttgcaaa ggagtatgaa aaacttaagt tggacctttg gaagaagtat gagcataatc
     3601 gtgatgctta tacagatagt aaactagatt ttgttaggga gtttactgaa aaggcaaagg
     3661 tgttgtattt taatagatat aattagaaat aggtacaatt agatatttat cttatatatc
     3721 gtattatatt ttttaagaaa gtaattaata gaaaaagggg ttgtttcaaa atggagagat
     3781 aaatcccttt ttcattgttg aaaggataaa aatatatttg atttcatgta aaaaagagtg
     3841 cctcatcaac taaaaagttt attttgagac acacacattt ttactaaggt aaataaaata
     3901 tcacatttga ttaaatttta agttaatgaa tatatgtgta aatatttaat tataagttat
     3961 tataatttta catagtatca aacaatctaa tttgtattat ataagataat ctaattttag
     4021 actaaaataa tctaatataa agaaataaag aaaattcatt ctcagtataa gagatatcca
     4081 attttccatt gtacttatta acaatttctc taatattctc aataccatat ccgtgagaat
     4141 tactatcttt tttatctgaa ataaatagtt cttgttttct attgatagga tttaccttgt
     4201 tgtttgtcaa taaatagcta aaatactcct tctcacaacg tgcctttaat accacttttc
     4261 tatttgacaa atcaggaatc ttcaaagatg cttctatagc gttatctaaa gaatttgaaa
     4321 aaatagtaca taaatctata ggttctatac ccaagtgatt gtctatatct atattaaagt
     4381 gcacatcaac accattttct tccaaacttg tatgacgaag atttaaaatt gagttaagag
     4441 cttggttttt gcaaaactgt ttgccactaa gagtacctat acctattttt aactgattaa
     4501 aatacttttt agctccagca atatcttcaa tttctaaata cgattcaatc atttgaaagt
     4561 gattattcat atcatgtcgc attttgcgta tttgaatttg ttgattttct agtaagttat
     4621 agtatttttc ttttagatga tttgcttgtt tttctatctt catctttaca ttttcaatca
     4681 atggctgtaa aagaagtatc acaccgatat ttgccagaat tgctgacaag atgattagat
     4741 aagtgccata aggtaagttg cctaagataa tattcttatt actacccata gggattaaat
     4801 tagtaggtgg cagaataata gcaattaata ttgataaaaa tgaagatata cataccatat
     4861 caataaaaat ccatatttta tttgataagt attgttttat attgaccaat ctattcttta
     4921 ggaaaatatg tattaaaaac caaaataata atttaattag agcagaaatt atctggacta
     4981 ttgaaagtat taaaatatac tcttttgagt gtataagttc ctcatatata gggttgttta
     5041 taaaaagaaa attaagccca attataatag gataaaatat catgacaact gatattatag
     5101 gaattaattt gcttttgcta catattataa gtagtataaa atatccgaat agtgtaaaca
     5161 aaatatttac ggggtcggaa agccatatta tcatttgact aataatagag tagcccaaaa
     5221 tcaatatgat tttgaaaatc caatgattcc taaagtctat aaaataggaa gtcaacgtat
     5281 aaagaaaaaa ggctatgaaa atattgatta atattagaat aaaatttatg cttgtagaga
     5341 ttaaagaata agtgcccatt taatatacct ttctattaat taagattctg gaaactaagt
     5401 tttaaaaaat taaatcttaa aaattaaaat tctggaaatt aaatttcaaa gaattgattt
     5461 tgaaaaataa aattctagag attgaacttc aaaaaattga gttttaaaag attgaaattt
     5521 taaaaattaa gtttcaaaaa attaaatttg aaaaaaaact ctaaaaacta agcttcaaaa
     5581 aattaaattt tgaaagattg aaattctaga aactaagctt taaaaaatta agtttgaaaa
     5641 aacaaaactc taaaaactaa gcttcaaaaa attaaatttt gaaagattga aattctagaa
     5701 attaatcttc aaaaaattaa atttgaaaaa acaaaactct aaaaactaag ctttaaaaag
     5761 ttaagtttca aaaataaaat tctagagatt gaacttcaaa aattaaggtt ctaacatata
     5821 ccttgtaaat tttcctacaa cctcttgata ttttttacgg cttataggaa ttttttcatt
     5881 ctgaactttg gcataagttt gagttatact atctacatgg tttagattga caatatacct
     5941 ttgatgcgta cgtataaaat tagagggaag aacagtttcc atatcattta attttccgta
     6001 gtattctaat gttttatcaa aggtaactac agttaatttt cttagctcac ttttaaagta
     6061 gaggatatct tttgtattaa ttttacaaag attagttcca atctgaactg taataaagtt
     6121 ttctttccat tgttctaatt tttttagtgc ttcttttaat actttacata ttttttcagt
     6181 tttataaggt tttaagatgt agttcaaagc tcctacatca tatccatgaa atacatagtc
     6241 agaaaaacct gtgacaaaga tgattatagt atcctttgaa tgttttcgta tagtttttgc
     6301 tatgtcaacg ccattacttt ttccaagttc aatatctaaa aaaataatat ctatgtaata
     6361 attttcgtgt tctagattgt atactagact ttctccattt gaatattctt ttatttggta
     6421 ggggtagccc cacaaatcca tttctcttgt aatgatttta ttcagcatgt ttctttgttg
     6481 tttttcatca tcacaaatac aaatcgaagt caatttttta tctcctttca aattttatat
     6541 ttatatagtt aagtatataa tatattttgc tacctagata tataaaatga aataacaatt
     6601 atatccagat agcaaaataa aattttttat attagtatac ttacacttaa aactattcta
     6661 ttaaacttat tctgaagtgc taatgatttt cttattaaaa aatctaacaa tctttcttat
     6721 tataaaatac aaatgctttt attattataa aaagtaatga ttttattact ttaaaaatct
     6781 a
//
LOCUS       DAMJWU010000149         6581 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMJWU010000149 DAMJWU010000000
VERSION     DAMJWU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.154"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            364..1638
                     /locus_tag="PC460_003408"
     CDS             364..1638
                     /locus_tag="PC460_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2602257.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1845..2162)
                     /gene="trxA"
                     /locus_tag="PC460_003409"
     CDS             complement(1845..2162)
                     /gene="trxA"
                     /locus_tag="PC460_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2602258.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2429..3181
                     /locus_tag="PC460_003410"
     CDS             2429..3181
                     /locus_tag="PC460_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF2602259.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3168..4049
                     /locus_tag="PC460_003411"
     CDS             3168..4049
                     /locus_tag="PC460_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2602260.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTILSGEIMY"
     gene            complement(4275..5630)
                     /locus_tag="PC460_003412"
     CDS             complement(4275..5630)
                     /locus_tag="PC460_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2602261.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2531 a          943 c          830 g         2277 t
ORIGIN      
        1 attatgttct aatcaatttc tttactattt ttacaacttt attagtcata ttcataatta
       61 tttctaatta gtgtttaaca ttttatgtaa aatttataaa aaaatgtata taaattaaaa
      121 taagtgacta tactaaaaat atgatacgga taaacaataa gttcaaccta tctcccccaa
      181 aatcaagtga gctacagttt cccccaactg tagctcattt tcgtgtaatt taataaacat
      241 ctcaacaaga attaattaac aaaacccctt ataaaaacat aacatatatt aaacaagctc
      301 ttgattaaaa cttgagcttt tttaataaaa aacagaatag tttattagat aaggggtttt
      361 tttatgcaat tcatggatgt atctaaatac attgaaggtg caaaccttaa agttaaaact
      421 aaaaatggtg aaactgttaa aagaatatat tttaacaact ctgccactcc attagtttta
      481 aaaaacgtag tggataactt aaactgtgag attccttggc ttacttatat taatgctcca
      541 ggaataatct cagaaaaaaa tactttaaaa tatgaaaatg tacgctctac tatcctcaaa
      601 cttattgatg gagacgaaga aaaggattct gttatttatg tcaaaaatgc tacagaagga
      661 ataaacttct tagcaaaatt ttttaaagat gaaaatccag ataaatttgt tattaccaca
      721 gcaatggagc atatggctaa ttacctgcct tttaaagtta attttcctac aaaagttgtt
      781 ggaattacag aaaaaggaga acttgattta tgtcaattag agaatatact taaaaattat
      841 gctggtaatg tatctctcgt tacagttaca ggagctagta atgtcacagg tataaccact
      901 cctatatatg aaatagcaag aatagctcat aagtatggtg caaagatatt ggtggatatt
      961 gtgcaagtaa ttcagcataa acctttttca atgatgccac atgaatgtga tgagcatata
     1021 gattttgttg tttttagtgc tcataaatgt tattctccac taaatggagg agctctggta
     1081 ggacctaaaa aattctttga aaaatttaat cctgtactat atggctcagg ttctaccaaa
     1141 tttgtaaatg ataacaaaat actatatgca aacgtacccc aacgttttga agcaggatac
     1201 ccagagtatt ttggaactct tgcaatggga aaagcactga acactttaaa taaaattggt
     1261 ctaaacagaa tttcaaaata cgaaagagaa ttatttttat atgctaaaga agaaatgagt
     1321 aaaataccta atataataat atatggagat aaatcaaata atgtaattat cccaaatata
     1381 tcttttaata taagtaatat gtattacaaa gatgtagcaa aatatttagt tgataacttt
     1441 ggaatagaaa ctggagctgg agtagtaggt gcagatattt atgtacaaaa attattaggt
     1501 ataaatccaa aagaggcata cgtcagattt atgagggaaa atcctgtagg tcttgttagg
     1561 ataagcttag gaatgtacaa tacttttgat gaggtggata gctttataca tgctattaaa
     1621 actttggctt ttaaataaga tttttctatt cttttttatc aataaataga gagtagtttt
     1681 gttttcacaa aaaaacaaat tatatcaaca gtcaatttca ttagcaatta gacactcatt
     1741 tgtaactata caaactattt tacactatac aaacaaaaaa ctcattacaa tgagtttttt
     1801 tgtttatata tgagatttat tttaattact tcaatttact ttaattacat atatgattca
     1861 attttgtttt ttaaattctc cttaggcata aaacctatta atctatctac aggttttcca
     1921 tccttaaata ttagcatagt tggtactgta gatacttcaa atttctgagc taattctata
     1981 ctttggtcta tgtccacctt caagaagttt gctttttcaa ccatctcatt tcctaaagct
     2041 tcatatattg gtgatagcat cttacaaggt ccacaccaag tcgcaaagaa atctacaact
     2101 actaaaccat ctttattttc tacttcattt ataaattcat tattatttat tatttttacc
     2161 ataatataca tctcctttat tatcttcttg atattaatat actacgccct atataacagt
     2221 tcaattaggc acttttttgt aaccgcctat ttttaataga tttaatatta tgataaatag
     2281 atttataaga ttaaatattt atgagactaa atatttctaa tatattttat ttttttctta
     2341 atatagttga cattttttcc cttaaattat aaaatcaaat taataactac ataggtagtt
     2401 actaatcttt tatcaaagga gtttctatat ggataatata attaatgaat tacaaaatat
     2461 aggatttact aaatacgaat ctcaaatata catatcatta ttaaaggaaa gccctttaac
     2521 aggatatgaa ataagtaaat tatcaggtgt accacaatca aaagtctatg aaaatataac
     2581 aaagctatta aacaataata taattatcaa tgttggaact gaccctatta agtacatacc
     2641 aataaaccct aatgaattat taaaaaataa gaaaaaagaa tttgacaatt ctatggataa
     2701 tctaaagaat agcttacaga tattaaataa aagtaaaagt gtagaatacg tattaaacat
     2761 aaaaggagat aaaaatatat taaaaaaagc aatagaaatg ataaaaaatg caaaacaaga
     2821 aattataatt tcttgccctt atgaagaaat tcttgaatta aaaaaagaac tcctaattgc
     2881 ttacaaaaaa aatattaata ttgaaatact acttttagaa aacaaaagtc tgaaaggtct
     2941 tgataatatt catattcatg gaggtaataa aactacactt gaaaataaaa tgctttacaa
     3001 aggaattatt ttgattgtag ataatgatga aatattgaca ggaaacttat cagaaggcag
     3061 tgaggccatt tcaatttgga caaaaaactc taacatttta tatattggag ttcaatacat
     3121 aaaacatgaa atttatattt cagaacaaat aaaaggaggt tataaaaatg aacaaaatta
     3181 agattaaaca agttgatgct tttacaacta taccttttgg tggaaatcct gctggtgtcg
     3241 ttactgatgc ctctaacatt tctgatgaaa ttaagcagaa tgtggctaaa gaaatgaatt
     3301 tatcagaaac tgcatttgtt tcagagtcaa atattgcaga ttttaaagta caatttttca
     3361 caccaggttt agaagttgat ttatgtggtc atgcaacaat aggtactttc catgcacttt
     3421 tggaagaagg gaagcttaat actaataaga gtatatttta tcaagaaaca aaagctggtg
     3481 tattatctgt agagcttaag aaaataaata atgaaaatat atttatgatg gaacagaata
     3541 cacctgaatt tgaaaattta gataattgca gaaaagaact tgcacaaatg ataggtgtgc
     3601 atgaagatag acttcttgaa tatcctatta taaaggtaaa tactggactt tggtggcttg
     3661 tatttggtct aaaatcttta gatgatttaa aaaatatctc tccagattta aataaaatca
     3721 aaaaatttag tggtgataat aatataattg gtataactcc attttgtata gaaactatag
     3781 ataaaaagtg tgactaccat ttaagatcta tagcaccata tgttggagtt acagaagacc
     3841 ctgtatgtgg aactgggaac gcttgcgtat catcttatat agtacatcac aatattttag
     3901 ataaaactag ttttattggt gagcaaggta actttataaa cagacctgga aaggtatatg
     3961 taaatataac tagagaaaac aatgacataa cttctgtaaa aattggagga agtgcttata
     4021 ctattttaag tggtgaaatt atgtattaat cccataccta ataaaaacaa ataaaaatac
     4081 tagctaacta aagctagtat ttttatttgt tatttttatt taataatcat actctaacaa
     4141 atattataat tatatatatc ataatattgt atttagaagt aaaattttat ttttattatt
     4201 aattagttat aaatcaatct aatacaacat aattacagta gattaaccta taaaacataa
     4261 taattgctta ctaattattt atcatcctca acaagtctac ttaatgtctt atcagataag
     4321 aataataata tcgctgcaac aaatagtatt actggaatta tagtatatac tttaatcata
     4381 tcaaatttaa gagtaaatgc atatatatat ccgtatcctt taccagctat aaaagtagca
     4441 aatgtccata ctcccataag aacacctaga agcttcttag gtgcatactt acttacaaaa
     4501 gagttaccaa gtggtgagaa taacatttca ccaagactta aaagaatacc aaacatgatt
     4561 atccatagta tactagcttt agcagtttca ggagctccaa cccctcttga aaattctgca
     4621 ccaacaagca ttaagaatgc aagacctaag aatattaatc caagacctgt ctttttaaat
     4681 aaactcatat ctccttgtgg tctactagca agtttaaacc ataatgctcc aagaactggt
     4741 cctaatacta tacatacaag tgaatttaat gagtcaaacc aagcaagagg aacatcaaat
     4801 cctccaacat tcatgtttac aaatgctgca ccataatcat aaactgcaag ataagttaga
     4861 taccagaata cccagaaaat tacagagaag aatgatataa gtataatagc ccaaattctc
     4921 ttcttatcca tgcttgtaag aggagactta tctttttctt caattttttc agcaactaca
     4981 ccttctttaa aaggtctctt tcctgcattc ccaaggaatc tccatccata tatgaaccat
     5041 attgcaccta ttacacaaag tacagctgct ataaagaaac attgagaaaa tccaagtaca
     5101 ccattttttg caaatgtttt aaggtaaaga ataccaactg ctatagtacc aataaaagaa
     5161 cctatattaa caaaagaata ctgtactgaa aatgctgtat caagttcttc ttgactatca
     5221 aaaagctgtc catttaccgc agatacgtta cctttaaaga atgcagtacc aattgaaact
     5281 aagattatca ttgcatttac catagaaact gtagttgcaa tagaaccaag atagtatcct
     5341 actcccatga taagcatacc tataggtata gtatatctag ctcctatcca tctatctgat
     5401 atatatcccc caattatagg tgcaaggtat gtgaatgcaa caaggttggc ttgcattata
     5461 gcagcttgtc ctttatctat tccaagacca ccatgaacta tagctgttgt aaggaatata
     5521 tatattaagt actttgaagc ataatatgct gctctttcaa atgagaaagt tgttgagcag
     5581 atataaaatc ccataggaaa tttacccttc ttttttactg ttgagctcat aattttaaat
     5641 cctttcttaa ttgtattttt attttttacg ttttgtccaa attctttctt ttatgaaaga
     5701 tataatatta ttattttgta aaacaatttg taattattat taagataatt gcgcatttat
     5761 ttgtagagta catattctca tggaataaaa ttaatacact tcttaatcat aaatttaaat
     5821 tatataaatt cactattttc agatatagtt ataaattaat atattctcat ctaataccat
     5881 gaaaaaacct tcgccatatg caattcttat tttttacgtg atagtatttt acaaactaaa
     5941 atcccccttt tgtagcaact tatgtaatta cattttagtg cttttttaaa ggagtctcga
     6001 ataaagcaac atatattttg ttcaaataat gtttttattt attttttaaa tttatatatt
     6061 acttcactca accaattctc aacttaaata actaaactac tatctattca cagattaggc
     6121 ttatttctta cacattagtt tataataaac actgtgaatc taaacataaa aactgttatt
     6181 ttaaagtaat tacccatata ttcgctttga tactatttat tgaggttata ctctttatgt
     6241 ataatatatt atttataaaa tacctcaaaa tatattattc ataaaatacc tcaaactgaa
     6301 aactttttca cgaatattta tttttcacct cccctttttt atttcaccat gtaaatcgta
     6361 ccataaatat gcacttatat gcaagaaaaa aatctattaa tcgacagtaa ataattagaa
     6421 aaattcaaat tatatttatc aagtatatta tataaaatat ttcctatgaa aatataaatt
     6481 aaattaatgt tcagaattgt ttttagatat gaaatcttga tttttttatt actttactta
     6541 tttgctcaaa tatatttttg tatgcagtat ataataatca a
//
LOCUS       DAMJWU010000150         6441 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMJWU010000150 DAMJWU010000000
VERSION     DAMJWU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.155"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(113..652)
                     /gene="hpt"
                     /locus_tag="PC460_003413"
     CDS             complement(113..652)
                     /gene="hpt"
                     /locus_tag="PC460_003413"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2602262.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(980..2287)
                     /locus_tag="PC460_003414"
     CDS             complement(980..2287)
                     /locus_tag="PC460_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF2602263.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(2712..4487)
                     /locus_tag="PC460_003415"
     CDS             complement(2712..4487)
                     /locus_tag="PC460_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2602264.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(4484..4621)
                     /locus_tag="PC460_003416"
     CDS             complement(4484..4621)
                     /locus_tag="PC460_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602265.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(4699..5268)
                     /locus_tag="PC460_003417"
     CDS             complement(4699..5268)
                     /locus_tag="PC460_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2602266.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(5452..6087)
                     /locus_tag="PC460_003418"
     CDS             complement(5452..6087)
                     /locus_tag="PC460_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2602267.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2049 a         1156 c          598 g         2638 t
ORIGIN      
        1 aattaaattg cataaaatta atttaatact actttaatta ctttttaaat aaaaaaacct
       61 caacacgatt aataaaaatc atattaaggc atccacaaaa ttatataatt gcttatctat
      121 cttcatcagt aacattgaat atataaggaa catttctata ataatcacca aaatcaaacc
      181 catatccaac cacaaactta tcttctattt caaatccaca ataatctggc actaaatcga
      241 cttttcttct gcttggttta tccaaaagaa cacagcattt taaactagca ggatttttag
      301 cttttagatg ttttaacaca aaatccatag taagtgcaga atcagtaata tcgtctacaa
      361 ccaaaacatc atatcctgct atatcagtag tcacatctga aactacttta acctttccag
      421 tactttcttc attgtttcca taactagatg tagtcataaa attaactcta agtggtaagt
      481 ttatatttct aactaagtct gcacaaaata taaagctacc ttttaataga gaaactacca
      541 taagattttt atctttataa tctttttcta taattgctcc taattccctt aatttatctt
      601 taattttttc ttctgaatac aatacttccc atttttttgt ttctatatcc ataaaaagac
      661 ctccaattgt aataatcctt cttaatttta taagagattg ctcacttttt caatactctt
      721 attcaatatt agtcaatatt attgagtttt tatgttcatt caacaattat taacaatgta
      781 tcttatattt taagtagttc atatatccac actaaattac atactatata ttttagacaa
      841 aaaaagaaaa aacttgcatt aacaagtttt ttctattata cataaattta tacataatca
      901 tattttatta gagggtacta attaaattta tttatgctat ttttgttata cctcataata
      961 caaaacatat tataaagtac tagtcaactt tattcatatc attcattaca tttaagatta
     1021 tctcatctgt tatagacata cctttgtcac ttactctacc taagttttgt atactttgct
     1081 caacacttga accaactata ccatttagtg gtggtacaaa acaatcttgc ttagctatta
     1141 tagcactttg tatagctgca gatgctgcag aagataattt taatgcacaa ccagctttcg
     1201 caccgtcaca tatcattcca cttaagtcag ctatcatatt ttctatagct ccttctattt
     1261 gcttttcagt accattcata agccaagaga taccagcagt tgctccagta gaagcagcaa
     1321 caccacatcc acatacagca gataatcttc ctgtatagtt cttaatataa ccagttacta
     1381 gatgagatat agctaaagct tttgctagtc tttcatcaga ttgtgggaat tttttattat
     1441 aagcaacgat tggaagaatt gctgttaatc catgatttcc acttccattt gaactcataa
     1501 caggcatttt tacccctgcc attcttgcat ctgaagctcc agcagttaac atcattgcat
     1561 aatttataac atcattacct aaaagacctt cttctataga ctttttaatt ccatatccta
     1621 caccaatacc agttttttgt tttaatccat attcagccat ctcttcattc atttttactc
     1681 catctagtaa aaattctata tctttaaaat caattccttc tacatttttt attaattctt
     1741 gtatagttac tgtatccatt aaactttcag ctttttctgc cgcatcattt gaagctgata
     1801 cttttggttc attgtctaat aatacttctc catctttttc taagaacgta aaattatcat
     1861 gtttagttct tattctcact ttagcaatat gattttttcc ttttaacaca acttctataa
     1921 atactttctc tcttgtatca gcaggtgtta ttgatagtgg agtattatcc atataatcct
     1981 cagctatttt aacttcctct tttgttagag tttctaacac acttaatcca ttttcagaat
     2041 gtccacctac tatacctaga gctgctgcaa tttttaatcc taatctttca gtacctggta
     2101 tacctacgca cataccattt ttataaatac taggactaac taacatacga ttttcaacta
     2161 tttcttctcc taataattcc tttgcctttg cacatgctag agctaaggct actggttcag
     2221 tacaccctac tgctggttta acttctgcct ttaacatttc cactaattct tttcttatat
     2281 ctctcattat atataaacct cccaattaag tttagattta gtactcaaat aattaattaa
     2341 ataatttctt atctatattt atagcaaaat acatgccata ttttttatat ctttttatat
     2401 ttttagtctt atattctata accattggta tgactgcctc tatttatgta ttttttttat
     2461 ttgtatagag tatttatatt ctttaaaatg gagaaacata tgacaatttc gcaaaaagac
     2521 aaaaaaaagt ctcaaaatga gattctatat cattttgaga cggctcaaat atattatttt
     2581 ttaacttata aaaaccattt tttaattcat aaaaaaagat tctgcaaaac acttatagta
     2641 taagtttaaa gctttcttta aagttatgaa tttagaaaaa tttaaatttt atatagcttt
     2701 ttattatatt actatgaaat tatattgtat ttttcaattt ttctatataa tgtagctctt
     2761 gatattccaa gtgctttagc tgttaattct ttatcttttt tataatcttt gtacttattt
     2821 aaagctttta ttatctcaat cttctctagt tcatcaagtg gaattatcct atcgctttta
     2881 acttctaact cttctacaga ggtattctta attttttgag gaattacatt cattgtaatt
     2941 attgaagaag aagacatatt cacactatat tcaataatat tctgcaactc tcttatattc
     3001 cctggccact tatattccat catagcatca acaaccctgc tatctatgtc aataacattt
     3061 ttatctaatt tttttgaata ctctttaacc atatgcttga ttataagtgg gatatcttct
     3121 tttctctctc ttaaacttgg taattttata ggtataacat ttaatctata gtataaatct
     3181 tctctaaaag tgccattttg aaccattttt tctaaatctt tatttgtagc agcaataatt
     3241 cttacatcta ttgaaacatt tgatttactt ccaattttat taagttcctt ttcttgtaat
     3301 actctcaata actttgactg taaatgcaaa ctcatatctc ctatttcatc taaaaatata
     3361 cttcccttat gagctacttc aaacattcca agctttccac cttttttagc tccagtaaaa
     3421 gctccctctt catatccaaa taattcactt tctaataaat tgtctggtat agcagcacag
     3481 tttacagcta taaaaggatt atctactcta ttgctatggt tgtgtatggc tcttgcaaat
     3541 agctccttac ccgttccact ttccccagta ataagtactg ttgatgttga tttagaagct
     3601 attagggctt cctttttaga atttcttata gctacacttt ctccaattat gttttccaat
     3661 tttattctat aatctttatt catcttattg tagtttttta tagcatctct tttatctata
     3721 aaatcaataa catatccctt taactcattt tttaatacta ttttattgat gttgtatatt
     3781 cccttcgaat catatccgta tcttttataa aaaaaactac aggatttgtc tttcttaaag
     3841 ttatttatag aaggtttctt tataaaatct aattcgacaa atatgttttt gccagttata
     3901 ctatccttta tattgaaaag ctctttaaat tttgaattat atttatcaat ttttccatcc
     3961 acatcaatag acacaacagc tttgtccata ttgtttaaca gtgtctctaa ttttttcttt
     4021 tctaattcaa gctcataagt tttaatttga gcttttaatt tattagatat taagtctgcc
     4081 attttgccta aaaagtttat aagaccactt ttattatttt ctataatctg tgcttgttcc
     4141 tttgtaaagg ctataaggcc tataacacca tgagagactc catcacatat aatgggacaa
     4201 cacacctctg caaactctac acaagcccca cttttatagc attccctaca tatatcacta
     4261 ctctttgggt catcaattag tattgtaatc tgctctgtaa gtgatttttt aaaagccgta
     4321 tatttatcta ctttctcacc tattttgtct atatatactc ctgtacctgc aatcctaaat
     4381 aactccttat ttactatagt tacatctata ttgagtactg atactatagc ttcagctata
     4441 ttttgaatat cagattttat atattctaaa cctatcataa tcatcattcc ttatttttct
     4501 ttaacataag tatttgtctt atttctttaa gaatcaagta tatcttattt aaagtaaatt
     4561 ggattgacat caggattaca aagagcataa caattattat taactcataa tttatttcca
     4621 tctaaacatc tccttataac aaaagactag gcttaaccta gtcttttata ttatatccat
     4681 atttatattt taactcattt atcttctttt tcccacataa atatatttta atttatttga
     4741 ctctttaatc ataccatcaa agtttaatct tgaaaatata ttaaatactt catgtggata
     4801 gtgaatcttt gcaacagaac cacaaaattt atgtttaaga tagtttaact taacttttaa
     4861 attctttaaa tttaaatcct ctaatataac ctctccattg ccctttaaca ctctttgtat
     4921 ttcagatgaa gctttttcac aatctgtaat aacattaaat tggtcaacta ttattatctt
     4981 gttgaactga ttatcatcaa atggtagaaa ctctaactta cctttaacta cattgcaatt
     5041 ttcattatac actagagaat aacagacatc ttcataatta ctattctcca gtatagttac
     5101 agaagttcca agacctctca atctcttgcc taggtttcct atatgaccaa caagcaatat
     5161 tttatcacct acattatact caattgtatc caaaaaacat tctaattcat cagatgtata
     5221 tttagcttta accggctgtt tatttatctg acataatatg cttttcacaa tgcttcacct
     5281 cttcatttcc tcaattataa attatgtaca gattacatta taataaatgg cgagcaacta
     5341 ttcaagagat atttcattag tattttttta aacaatcttt atacatttta atatgatatt
     5401 ataccaaatt tattaggatt ttattcataa aaatcagtct attttttatg tttattcatt
     5461 gttattttca tccaattttt ttattttcga tatggatact tcatatgcca tttttttgac
     5521 cacattattt tcatctattt ttttctcata ttctctactt tgaaccctac cccaaagctg
     5581 tattctatca ccaaccttta gattctttgc aaatttagca tttctacccc aaactattga
     5641 tggtatataa tcagatttat tataaggtct gtttatagca actaataaat cggttatttc
     5701 ccttcctaaa ggtgtcttcc tatacacagg ttccttacat acatatccat ctaaaaatat
     5761 actattaggg tctttatttt cctcgtctat gctttttatt tctctgacaa acactgtaag
     5821 cactaatcta tttttattat ttaatgtttt gttatatgat cttaattggc ctacgacatt
     5881 gactaaattt tgtttatcaa ggtcaatatc ttggagaagt ctttctgata cagtcattgg
     5941 aagtatatca aaagaatcgc ttagtctgtt tatttttatt ttcatattat aaaatttttc
     6001 tccaaatatc tcatggctaa attctagctt attgtctaac tcccctctta agtttactac
     6061 gttattctca tctttaacag ctatcatatt ttatccctcc acaattgtta attcttatat
     6121 ttctggtatc agcttatata ataaatatat ttataaggga tgtattttat tacaaatctt
     6181 tgattgctgt ttaaaagttt ttatttagtt ataaatttca tttaatattt cacttttata
     6241 attataaaat tattatcttt ttgtaccaca tatactaatt aaactaccta tttcagtttt
     6301 gtattaatta taaaaatcct attatatagt taataagtca tatataactt aaatttattc
     6361 actctatata agcaacttca acgaattaaa tttattcaaa taattcagat gaatattgag
     6421 tcattataaa cttatttttc t
//
LOCUS       DAMJWU010000151         6381 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMJWU010000151 DAMJWU010000000
VERSION     DAMJWU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.156"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            180..1505
                     /gene="cdeA"
                     /locus_tag="PC460_003419"
     CDS             180..1505
                     /gene="cdeA"
                     /locus_tag="PC460_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF2602268.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFISSLLVFIIALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1600..2394)
                     /locus_tag="PC460_003420"
     CDS             complement(1600..2394)
                     /locus_tag="PC460_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602269.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2395..3270)
                     /locus_tag="PC460_003421"
     CDS             complement(2395..3270)
                     /locus_tag="PC460_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602270.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3274..3642)
                     /locus_tag="PC460_003422"
     CDS             complement(3274..3642)
                     /locus_tag="PC460_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2602271.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3825..4484)
                     /gene="deoC"
                     /locus_tag="PC460_003423"
     CDS             complement(3825..4484)
                     /gene="deoC"
                     /locus_tag="PC460_003423"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF2602272.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4520..5707)
                     /gene="murJ"
                     /locus_tag="PC460_003424"
     CDS             complement(4520..5707)
                     /gene="murJ"
                     /locus_tag="PC460_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2602273.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5784..>6381)
                     /locus_tag="PC460_003425"
     CDS             complement(5784..>6381)
                     /locus_tag="PC460_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2602274.1"
                     /translation="FENTNNQIGLDLGIKEFCISSCGEFIENPKYFKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLTTKLIKENDIICIEDLQVKNM
                     IKNHKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKN
                     LNIREWICPSCNETHDRDINASRNILKEGLRLITIQNK"
BASE COUNT         2108 a          999 c          698 g         2576 t
ORIGIN      
        1 ttcttaagaa acagttagat tttactaata taagtttatt aattagcatt atactaattt
       61 atcttaatat gattttttgt tgattttaag ctgtttatcg tataaataaa atttttcttg
      121 actatatagt tactatatag cttattatat atagagagta ataaaaggag gtaaaattta
      181 tggaaaattt atttacaaga aaattcacta cttttgaatt tctaaaattt gtttctcctg
      241 caattatatc catgatattt atatctttgt atacaataat agatggcatc tttgtatcga
      301 cattagttgg ttctgatgct cttgctagta taaatattgt actacctata attaaccttg
      361 tttgtggatt tggaataatg atggcaactg gtggaggtgc tatcgtttct atacgtatgg
      421 gtgaaaatag acaggatgaa gccaactcta cattttcttt tatagttttg ttttcattga
      481 ttgttgggat tttattcaca gtaatctcat atttctttat caaagaaata tctattttgc
      541 ttggtgcaac agataagtta ttaccatatt gtataactta tggtaaagtt atgattttat
      601 gtacaccatt ttatatttta aaatttatat ttgagtactt tgcaagaact gatggaaatt
      661 ctaaatttag tttatttcta tcagtcattg gtggtgtaac aaatataata ttggattatg
      721 tatttattaa atattttgga atgggtcttt taggagctgc agttgcaact gctataggta
      781 ttattttaac ttgtgtttta ggtattattt acttcttatc taataaatct acactaaaat
      841 taagaaaacc aaaaaccgat tttagactta taagagatac tatgataaac ggttcttctg
      901 aaatggttac agaattatct acaggaatta caacattctt atttaatgta gtagctttaa
      961 aattagcagg agaaaatgga cttgctgctc ttactatagt attgtatgct cattttttaa
     1021 tgacatcagt ctatctagga ttcgctgctg gagtgtctcc attaataagc tataattttg
     1081 gtgctgaaaa cagtgataaa ttaaaagaaa catttaaaca ttctctaaaa tttatattta
     1141 tttcttctct tttagtgttt attattgctt tagtatttgc accatttatt gttagggtct
     1201 ttgtaaatcc agataacaca gtatttaaac tagccttaca aggattaaaa atatttgcat
     1261 ttgctttttt gtttgttggt ataaatatat ttgcatcagg attttttaca gcatttcaca
     1321 atggaaaaat ttcagctatt atatctttta gtcgtgcctt tgtttttata atcataggaa
     1381 tcataattct tcctcctatg ttaaacatga ctggattatg gcttacagtt ccatttgctg
     1441 aggttataac catatttata tctattctat ttataaaaaa atataaatgt agatataagt
     1501 attaaaaatt aatacttaaa atcaacattt attaaaacta ataaacctgc tttaaaatac
     1561 aaatttacaa atttgatttt taagcaggtt ttattttagt tataataagt catttgcata
     1621 ctctttaatt ggtgcttttc taagtaataa aattataaat attatgcaaa taactataat
     1681 tcctaccttt gttccaaata aaataaaagg attctgaaat gaataaataa ttttttctaa
     1741 atacaatagc attttatgca tatttttagt tgcaaaatta actatataaa ttaccattaa
     1801 tatcggtaat cctatcaata tagtaatact agtaactttt ctaactctat atgtaaaagc
     1861 tccaagtaac actcctataa aacctataca tatttcattt atacatattt gtacaaaata
     1921 atttaaataa gttagtggca tacttgatgt tgaaggatct atcagtgctg atatagatgt
     1981 ccaaatcata tctctcttta gtaaaactga tagttctata ctgtaacctg ttataatttc
     2041 tataaataac ttcgataaaa aaatcaaaac tatacctaaa atagaaatca caaagcttaa
     2101 aataattaaa tctatgacaa tagcttttat acaacttttt ctatctgctc ttatacttaa
     2161 agctcctgaa aagtctttat ttgctatatt tactccatac acaaatagca gtatagttat
     2221 atatgttaaa ataggattca caatcgtgga taaattagtt ttagatgctt ctccaaaaat
     2281 cttgagaata aacactggca aaatattcat aacaattccc acaatcataa atataattat
     2341 atacaattta gtatttttat taaaaaattt tatatatggc tttaattcat tcaattacaa
     2401 ttcctccttc ttagtcatgt taacaaacat atcttgtagc cctatgtatc ctacatcaat
     2461 atttgaattt ttaagtattt tttcatcatt ttcactaaaa tcaccatagt aaaaataagc
     2521 aactgcattt ccaaatgcct ttttaggttt tatatttttt atacattcta atttttctaa
     2581 ctcttcttta tttccagtaa tataatgagc tttttgtttt atgacatcaa tttcttcatc
     2641 tataataatt tttccctcat ttaatattat tacatgctcc agtaaatcat ctatttcatc
     2701 aatcaaatga gttgatatta ttattgtcct tggatttttt atataactat ctaaaattat
     2761 gttataaaac tcttgtctat ttactgcatc caaaccaatt gttggctcat caaatatggt
     2821 gatagctgaa ttagaacaaa tacctattat atttgaaagt atagttttca tacctcttga
     2881 cagttgttta tacttttttt tagtatttaa atcaaaatat ttgcataatt ctttttctaa
     2941 gcctttatca taacttttat aaaaactaga agaaaattca aatatctgac ctactttaaa
     3001 atctggacta taaaattctt tttccctgac aatacaaact tcttccaaaa cttttatatc
     3061 ttcttttggg ttctttccta atatttctat ctcaccttca ttttgaatta actgatttac
     3121 cattatattt aataatgttg tttttccagc tccattctta cctaataaac catatatctt
     3181 atttttttca aagtttagag acatattatt aaaaatattt tgcttcttaa aactgtgaga
     3241 taaattttct atcttaatag aactactcat atattaatcc tccttgaaat taattataat
     3301 atcaaccaat tcttctctat ttatttctaa tttgttagct tcttgaagta ggttttttac
     3361 aaaattatgt ttaaaatttt cttttctagc ttcttttata atagtttttg caccatctga
     3421 tacaaacatc cctatccccc tcttcttata taaaatattt ttatcaacca atatatttat
     3481 accttttaat actgtagctg gattaatttt ataaagcttt gaaagctctg ttgttgatgg
     3541 aacctgttct tcttctttta ttccatttga tagtatctca tcttcaattg ctcgtgctat
     3601 ctgaataaat ataggttctt cattatttaa tactaatttc ataggttcct cctttctcca
     3661 ttagttagtt actcaactaa ttaactatat aacttataat atcttgaaaa atattttctg
     3721 tcaatactaa ataaaaaaca gagggaaaat tccctctgtt ttttgttctt tatatttata
     3781 attaaatttt gatttttgta atcctatccc ataagttaaa aactttaata atctgttgta
     3841 tcttcatttt ttaatatttt acatattgag ctagttccta acctttctgc tcctgcctct
     3901 ataaattttt ctgcatcaga aattgattta actccccctg cagcttttat ttttacattt
     3961 tttccaacat gctctttcat aagctttata tcttctaaag tagctccacc tgttcccatt
     4021 ccagtagatg tttttataaa atctgaacct gacattgtta ctatttcaca catttttatt
     4081 ttttcatctt catctaaata acatgtctct ataatgactt ttaatacttt atctcctata
     4141 gcttttttta tttcttttat ttcttttcct atattatcat aatctttatt ttttatatct
     4201 gaaatgttta caaccatatc aacttcatct gctccatttt caatagcatc ttttgcttca
     4261 aatacttttg tagcagtagt ttgatatccc aaagggaacc ctatcactgt acatatcttt
     4321 attttacctt ttagatattc tgatgctctt ttcacatatg atggtggtat gcaaactgat
     4381 gctacactca tatctacagc ttcatcacat aaaattttaa tatcttccca tgtagttgta
     4441 gcttttaaaa tagtatggtc tactgttttt aatatatgtt tcataaaatc ctcctaaatt
     4501 tataattaat ataattagat tactttttta aatgcaattt tctctttgca atattcagta
     4561 tcatatcaac ctcttcaact ttaaaaatag tcattagtag tgtataaatt ccacctccaa
     4621 ctataactga aatagctagt gagacaaact cattaaatgt tcctactccc aaaattccaa
     4681 atataaattt atacgtaaag tatgataaaa cacccataat taaagatgca actaccgatt
     4741 tcaaagttgc ttttattatt ttatcttgtc caaaatatcc aacttttctc tttaggttca
     4801 agaagagtaa caatatacat gcaattgatg atgaactagt tgcaagagca agaccaccat
     4861 gagccattgg ttttattaaa actaagtcca atactatatt tacccctaca gatataatcc
     4921 catttaccat aggtgttttt gtgtcttgta atgaataaaa aacctttccc aaaatatctc
     4981 ttaagccaaa tgcagtcata ccaactgcat aaaaaattaa ggcagttgct gtcatttggg
     5041 ttgccctcgc atcaaaagca cctctctcaa aaatgattct aacaacagga gttgcaaaaa
     5101 aaatagaagc aactgaaata ggaatcatag aaattattat catatttata ctagatttaa
     5161 ctgaactagt aaacttcttt tgattatttt ctgcacttaa cttagataac ataggataca
     5221 taacagatac tattgaagct gtaaatgtcc ctgtaacaaa accatttaat ctatctgcat
     5281 aagtaagtgc tggaatgctt cctttaacta atgttgaggc taaagttgta tctaccaatg
     5341 agttcacttg attaacagca acacctataa atactggtga aagtaatgct agtaatttaa
     5401 taaggctatc atctttaaag tttaagtagt ataagtattt gtagttcgtt tttttaacaa
     5461 aaaacatgta aaagagcagt tgaactacca tagcaaccac agcaccaatt ggaagtatgt
     5521 atggtccaaa tatggtactt aacattattg aaataattat tactatatta tagggtatac
     5581 ttccaaatcc aactacaata aagttctctt taatttgtaa aaatgctgac ataacacttg
     5641 taatacctat gaatattatt cctgttctga acctcccatg actaaagtca cagggttcct
     5701 gcttcataga attttgcata ctaaaatatg tcttactaat tctccacagg ctatccccgt
     5761 agttcctacg gttcttacat acgttattta ttttgaattg ttattagtct taatccttct
     5821 tttaatatat ttctacttgc atttatatct ctatcgtgag tttcattaca actaggacaa
     5881 atccattctc ttatatttaa gttcttaact tcctcgtttt tatacccaca tttattgcat
     5941 atttgtgaac ttgcaaagaa tttacctacc tttacaattt gtctaccatg ccaattagcc
     6001 ttgtattcta attgacgaat aaactctgac catgatacat cagaaattaa gcgagatagt
     6061 ttatgatttt taatcatatt ctttacttgt aaatcttcta tgcaaataat atcattctct
     6121 tttattaatt tagtagttaa tttttgtaga aaatcatttc tttgattagc tatatgttct
     6181 tgaagtcttg caacttttaa tcttgcttta tttctattta aactaccgat tgtttttctc
     6241 gataattctc tttgtaattt agcaagtttg tttaatgact tcttaaaata cttaggattt
     6301 tctataaatt ctccacaact tgagatacaa aattctttaa ttcctaaatc taaacctatc
     6361 tgattgttag tattttcaaa t
//
LOCUS       DAMJWU010000152         6368 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMJWU010000152 DAMJWU010000000
VERSION     DAMJWU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.157"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            293..1237
                     /locus_tag="PC460_003426"
     CDS             293..1237
                     /locus_tag="PC460_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF2602275.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1259..1885
                     /locus_tag="PC460_003427"
     CDS             1259..1885
                     /locus_tag="PC460_003427"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF2602276.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1919..2968
                     /gene="hisC"
                     /locus_tag="PC460_003428"
     CDS             1919..2968
                     /gene="hisC"
                     /locus_tag="PC460_003428"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2602277.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2971..3549
                     /gene="hisB"
                     /locus_tag="PC460_003429"
     CDS             2971..3549
                     /gene="hisB"
                     /locus_tag="PC460_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF2602278.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3551..4156
                     /gene="hisH"
                     /locus_tag="PC460_003430"
     CDS             3551..4156
                     /gene="hisH"
                     /locus_tag="PC460_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF2602279.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4153..4875
                     /gene="hisA"
                     /locus_tag="PC460_003431"
     CDS             4153..4875
                     /gene="hisA"
                     /locus_tag="PC460_003431"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF2602280.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4857..5615
                     /gene="hisF"
                     /locus_tag="PC460_003432"
     CDS             4857..5615
                     /gene="hisF"
                     /locus_tag="PC460_003432"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF2602281.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5643..6311
                     /locus_tag="PC460_003433"
     CDS             5643..6311
                     /locus_tag="PC460_003433"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF2602282.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2604 a          540 c         1258 g         1966 t
ORIGIN      
        1 gtaaatttag aatattaata aaaattattt gggaggaaat tatcatggtg agattaaata
       61 tagtaaaatt aaatacaaaa ttaaatcatc accatcatca tagattaaag ggattgtaat
      121 atgaaattaa ttcatagaca caagccctgt ttttagtgta attggtcttg tatctatgat
      181 gggaagtaat tttaagagaa aattatagcc atataggtat aaagcctatg tggctttttt
      241 attgttttaa atttgcaaat ataagagtaa atttaatgaa gagggtgaga atgtgaaatt
      301 tttgaggatt gaagatgagg ttatttattc taaaaaaaga tataaattaa aaagggaaat
      361 agataaactt tttttggatg aagaatattt tcaaatagaa ccacaattat ttgaagaata
      421 tgatgaattt acaactataa ataataaaat tccagaagaa tctatggtaa aggttgtaaa
      481 tggaaaagta atggtactta gagctgatat aactacaagt ataataaaaa gccttgtacc
      541 aagatgggaa gatggtttaa agcttaagct tttttataat tcatcaatat ataaaaataa
      601 aaatacagtt ggaataaaag agataagaca aataggttgt gagtatctag gagaagcctc
      661 tgtagaagca gatagagaag tagtgaaatt agctcttaag atacttgaaa aatacaataa
      721 taattttatt ctagaggttg ggagtagcaa gtatattcat ggtttgttag aagaattaaa
      781 tttaaataag aattgtgaaa atcaaattaa aaatttacta tatacgaaga acactcatga
      841 actaaaagta tatattgaag aattaaaaat taagcgtgaa gtaaaagagt tattatcaaa
      901 tattttaagt ttacaaggga atttatataa tgttattgaa aaatcaaaca agttctattg
      961 taataataag atgaagcaag ctcttgaaga attaaagcaa gtaaataact tgattgagga
     1021 atgtaatttt ttagataagg ctagatttga tttatcaatg attactatgc ttgactatta
     1081 tgaaggaatt atgtttagag gatactaccc aaactcatat aaagaaattc ttagtggtgg
     1141 aagatatgat tctttaacta aagagtatgg aaaagaaatt ccagccattg gatttacact
     1201 aagtgttgat gagttaatga agtatgtata taagtaaagt ttagaaggag ggattgcttt
     1261 ggattatttg actatagcac tagcaaaagg acgaattgaa ggggaatcat ttaaaaaatt
     1321 taaaaaaatg ggtctaggag acagtataga cacagatacg agaaaactaa tttttaaaga
     1381 tgaagaaaat aaaattatat atatacatgt aaagccatca gatgtagtta catatgttga
     1441 aaaaggagtc gctgacctcg gtatagcagg taaagatact attttagaga atgagacaga
     1501 tgtatatgaa atatatgatt taggttttgg aaaatgtaaa tttgctgtag caggattaaa
     1561 gggagatagc atatatagag aagatgaata tttaaaagtg gcaacaaagt atcccaatat
     1621 agcaaaaaag tattttaaag agaaaggtca aaaaattgaa ataataaaat taaatggttc
     1681 tgtagaacta gctccaatag tagggttgtc agatgtaatt gtagatatag ttgagactgg
     1741 caatacactt aaagcaaatg gtcttgaaat attagaagac atatgtaata taagtgctag
     1801 aattatatca aatagagcta gctatagatt taaatatgaa caaattcaga atataattcg
     1861 tttatttgaa gaacttgata attaaaatat aattgagctt tataaggagg gatttaagtt
     1921 gagagaaaaa gaaagcataa gagagttaaa gggatatgaa ccaaatcacg taaactgtaa
     1981 agtaaaactt gatgcaaatg aaggaagtaa gaggttgttt aaatacctta ttaaagaaat
     2041 ttcagatagt gatatagatt taaatttata tcctgaagat tcttatagtg atttgaaaga
     2101 atctataata gattacataa atatttctgg agtaaataag aaaaatcttc tagtaggaaa
     2161 cggttcaagt gaaataattg atttaataat acatactttt gtagataaag atgaagtaat
     2221 attgagtttc tctccaagtt ttagtatgta ttctatatat agtcagataa atgggtctaa
     2281 atttataggt gttgaaagtg atgaaaattt agttataaat atagataatg tgattgaaaa
     2341 agtaaaggag aataatccta aaatagtaat tgtatgtaat cctaataatc caactggaac
     2401 aatattaaag agggaagaga taataaagct tttagattca actaatagtc tagtggtttt
     2461 agatgaagca tatatggatt ttggagaaga aagcatgtta agtgatgttt ttaagtatga
     2521 taatctaata gttcttagaa ctttatcaaa ggcatttgga ttggctggaa taagaacagg
     2581 atatatgtta tctaatagta gtttaataaa ttctgttgag aaagtaagac ctccatataa
     2641 tttaaattca ttatcagact ttattgctac aagagcactt agaaataaag atgtggtaaa
     2701 ggcttatata aaggaagtaa aagaggaaag agaagtttta tataaagaaa tgattggtat
     2761 gggaattaaa gcatataaat cacaagcaaa ttttatatta ttttattctg aaatcgaaaa
     2821 tttatcacaa aaacttattg atagaggtgt tttaataaga aaatttggtg gaaagttaga
     2881 aaattattac agagttacaa ttggtgataa agaagaaaat tctatgtttg ttggagctat
     2941 aagagatatt ttgaaaaagg agaaataggt atgagaattt ggaaggtaga aagaaatact
     3001 cttgaaacac agattttagt agaattaaat attgatggtt ctggtaaagc tgaaatagat
     3061 actggaatag gatttttaga ccatatgctt accttaatgt catttcatgg caagttcgat
     3121 ttaaaagtga tttgtaaggg tgatacatat gtagatgacc accatagtgt agaagatata
     3181 ggtatagcta taggtgaagc atttaaaaat gctctaggtg acaaaaaagg aatacgaaga
     3241 tattctaata tctatatacc tatggatgaa tctctatcaa tggtagcaat agatataagc
     3301 aatagacctt atcttgtatt taatgctaaa tttgataccc aaatgattgg tagtatgagt
     3361 actcaatgct ttaaagaatt ttttagagct tttgtaaatg aatcgagagt aacattacat
     3421 atcaatctct tatatgggga aaatgaccat cataagatag aaagtatttt taaagcattt
     3481 gcaagagctt taaaagaggg tagtgaaata gtttctaatg agatagcatc ttcaaagggg
     3541 gttttgtaga atgaatataa tagtagacta tggtcttgga aatatagatt ctgtgtctag
     3601 gggttttaga aaagctggaa tagaaactaa aatttctagt gatatagatg aaataaaaca
     3661 ggcagattca cttatacttc caggtgttgg agcttttaga gattctataa gtgccttaga
     3721 taaactagga ttgataccaa taataaagga gcatgtttct aagggtaaat ttatgatagg
     3781 tatatgtcta ggaatgcaat tactttatga aaaaagttat gagtatggag aatatgaagg
     3841 attaggactt ataaaaggaa gtattgataa actggatata agtttaaaag tgccacatat
     3901 gggatggaat aatctaaaat ttaataaggc aaatgatgat atacttaaat atataaatga
     3961 agatgattat gtatacttcg ttcactcata ttatgcaaat tcatcaaatg aagaactaat
     4021 tgcattttca gaatatgaaa agaaaatccc tgctattgtt agaaaaggca atgtatatgg
     4081 tatacaattt catcctgaaa aaagtggaga agtagggtta aatatattaa gagcatatgg
     4141 ggagatgata aaatgataat atttccagca atagatataa aagataataa gtgtgtaaga
     4201 cttacacaag gagaatttga caaagtaaat gtatattatg ataatccatt agaagtagca
     4261 tataaatgga aaaatgaagg agcagaatat atacatatag tagatttaaa tggtgctaga
     4321 agtgaatttg gtgtaaatac aaaaataatt gaggacatag caaacaatat agatatacct
     4381 attcaagttg gcggtggagt tagggataag gaaaaagtca aaagtttaat aaatgctggt
     4441 gttactagag taatattagg tagtatagcg attgaaaatt tgaatttagt tgaagaattg
     4501 gtcaatgaat ataaagagaa aattgtagtt tctatagatg ctaaagatgg aaaagtagca
     4561 gttaggggat gggaagttgt aagcaatgta gactcattaa cattatgcaa acaacttgaa
     4621 aagataggtg tacaaactat tgtttataca gatatatcaa aagatgggat gttgcaaggt
     4681 ccaaattttg acatatatga aagaatagca aaggaaacat cactaaatgt tattgcttct
     4741 ggaggcgtaa cttctataga agacgtaaaa agactaaaag ctatgaactt atatggtgca
     4801 ataataggta aagcgcttta tgataaaaag atagacttta aggaggcaca acaattatgc
     4861 ttactaggag aataatacct tgtctagatg tgagaaatgg aagagtcgta aagggaaaaa
     4921 aatttaaaga tatagtagat gttgatagtc cagaagtact tggaaaattt tatagtgatt
     4981 gtggtgcaga tgaacttgta ttctatgata taactgcatc aaatgaagag agaaagacat
     5041 ctttagaatt tgtgacaaaa gttgcagaaa atataaatat accattttgt gttggtggag
     5101 gagtaaataa attagaagat ttcactgata ttttaagaaa aggtgctgat aaagtttcta
     5161 taaattcatc agcagtcaaa aatcctgaat taataaggga agcttcatta aaatttggag
     5221 cacaatgtgt tgttttatcg atagatgcaa agaaaaatga agagggttca tggagcgtat
     5281 acgtaaaagg tggaagagaa aaaactaatc tggatgcaat tgaatgggct gttaaaggtg
     5341 tagaacttgg agcaggagaa atcgttgtaa atagtatgga tgaagatgga atgaaaaatg
     5401 gatatgatat agaattatta tcaaagataa cttctttagt aaatgtacct gtcatagcat
     5461 ctggaggcgc tggaaaaaag gaagattttt atgaggcggt taataaatcc aatgtagatg
     5521 gaattttagc agcatctgtt tttcattttg gcgagataaa aataaacgat ttaaagaaat
     5581 atttaaaaga catgggtgta gaagtaagat tataagttaa atactaaaag gagattagaa
     5641 atatggataa taagtgtaac aatgtataca gtgatgaagt agagaaattt ataagaagta
     5701 taaagtttga tgataaaggg ttagtccctg ttgttgttca agaggtagtt agcaaagatg
     5761 tactgatgtt agcatatatg aataaagatg ctataaagaa aaccttaaaa gataaagtag
     5821 cttgctattt tagtagaagt agacaagaac tttgggttaa aggtgagaca tctggtaata
     5881 ctcaaaaagt agttaagatg tcttatgatt gtgatgtaga tactatacta ctttttgttg
     5941 aacaaactgg tgtagcttgt catacaggca attattcttg tttttacaga gacttgtttg
     6001 atgatagttc aaaaatggag cttgaagtac aaacaaatat attgaaagaa ttatatgatt
     6061 taatcaatga aagaaaaaat aatccagttg aaggttctta tactaattat ctttttgaaa
     6121 aaggaataga taagattctt aaaaaagtag gtgaagagag tagtgaggta attatagcat
     6181 caaaaaatac cgataaaagt gaacttattt acgaaattag cgatttagta tatcatacat
     6241 tggttttaat gatagaaaaa ggtgttgaaa ttgatgaaat aaaaaaagaa ttacttaaaa
     6301 gaagaaaata gaataagata gattaatggg taataagaaa atataccact tttaataaaa
     6361 agtggtat
//
LOCUS       DAMJWU010000153         6226 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMJWU010000153 DAMJWU010000000
VERSION     DAMJWU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.158"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            185..1207
                     /locus_tag="PC460_003434"
     CDS             185..1207
                     /locus_tag="PC460_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2602283.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1267..2274
                     /gene="gap"
                     /locus_tag="PC460_003435"
     CDS             1267..2274
                     /gene="gap"
                     /locus_tag="PC460_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2602284.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2496..3698
                     /locus_tag="PC460_003436"
     CDS             2496..3698
                     /locus_tag="PC460_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF2602285.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3724..4467
                     /locus_tag="PC460_003437"
     CDS             3724..4467
                     /locus_tag="PC460_003437"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF2602286.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4476..6008
                     /locus_tag="PC460_003438"
     CDS             4476..6008
                     /locus_tag="PC460_003438"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF2602287.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         2481 a          721 c         1258 g         1766 t
ORIGIN      
        1 aaaagaaaaa atactgtgat ttgaaaatgg tggagaaata ttttataaaa taaaaaaata
       61 aaaaagtttt tgaaaaacta ttgaaatcta tacgcaaatc ctataaaata aaaatatctt
      121 gtgggactta aataaacact agggacgaaa aaagtcccga aaggaaagca ataaggagta
      181 tgttatgaag aacttattag aaattcaaaa gaaattgatt ccacaggcaa ttgaacttat
      241 ggaaagaaga tactctattt taagacaaat atctttaagt gaaccaatag gtaggagaac
      301 attatcaaat gtattagaga ttagtgaaag aataatcagg tcagaaactg agtttttaaa
      361 agaacaagga cttatagacg tagctgtatc cgggatgact atcactaaag aaggttctga
      421 tttgttagat aaacttaagg acatcatgag tgacatcatg ggattgtcta agttacaaga
      481 tagagtcaga gaaaagcttg gaataaaaag ggtaatatta gttcctggaa gctgtgatga
      541 aaatggaagt ttactaaaag atgtaactag atatggatgc gagtatttct taggtatttt
      601 aaaagatgga gatatcgttt caataacagg aggaagcact atgttggagt tttcaaacgt
      661 tattaaaact gataagagat acccaaactc tacaatagtt ccagctagag gaagtatggg
      721 aactgatgta gagatgcaat ctaataatat agttgcttcg ataagtaaga aattacattc
      781 taattgcaaa cttttacata taccagatga gttggatgaa agtgcaatga aaactcttag
      841 ccaagtgcca gagattaaga caactttgga gtatattaaa agaacagaca ttttagtttt
      901 tagtataggt agagcagatg tcatggcaaa gagaagaaaa cttccagaag acaaggttga
      961 tgagatatta tctaaaaatg cagttggaga agcatttgga tattatttca acaaagatgg
     1021 agaaatagtt tacaaactga acactgttgg aattgattta gaaacagtta aacaagtaaa
     1081 agaaactatt gcaatctttg caggtactga aaaagtagaa gccttattag ctatatcaaa
     1141 tatgcataaa aacatggttc ttgtaacaga tgaagaaagt gcttataaga tattgtcatt
     1201 attataaaag tcaagaccta gcacagagct agaaaacata catttacttt ataggaggta
     1261 gcgaaaatgg ttaaagtagc tataaatgga tttggaagaa taggaagatt agcattaaga
     1321 aaaatgatgg agcaacaaga taagttcgaa gttgttgcaa taaacgactt aacagacgct
     1381 aaaatgttag ctcatttatt taaatatgat acagctcaag gaagatttaa cggagaaata
     1441 gaagttaaag aaggtgcttt tgtagtaaac ggaaaagaaa taaaagtaac agcagaaaga
     1501 aatcctgctg acctaccatg ggctgaatta ggagtagata tagtattaga atgtacagga
     1561 ttctttacat ctaaagataa agctgaagct catatacaag caggggctaa gaaagttgtt
     1621 atatctgcac cagctactgg agatttaaaa acaatagtat tcaacacaaa cagtgatata
     1681 ttagatggtt ctgaaacagt tatatcagga gcttcttgta ctactaactg tttagctcca
     1741 atggctaaag tattaaatga taaatacggt atagaaaaag gattaatgac tactatacat
     1801 gcatacacta atgaccaaaa tactttagat ggtcctcatc caaaaggtga tttaagaaga
     1861 gctagagctg ctgctggaaa catagttcca aacactacag gagctgcaaa agcaataggc
     1921 ttagtaatac cttcattaaa aggaaaatta gatggggcag cacaaagagt accagttgta
     1981 actggttcaa taactgaatt agtttgtaca ttaggaaaaa atgttacagt tgaagaaata
     2041 aatgcagcta tgaaagaagc ttcaaacgaa tctttcggat atactgaaga aatgttagta
     2101 tcttctgaca taatcggaat aagctatggt tcattatttg acgctactca aacaaaagtt
     2161 atggaagtag atggaaaaca attagttaaa gttgtttctt ggtatgataa tgaaatgtct
     2221 tatacttctc aattaataag aactttagga tactttgcac aattagcaaa atagtcagtt
     2281 ttgagaaaat agaaaagtag attgacaaag tccgagtttg tagtttaata ctacggactc
     2341 aggacttttt ttaagaaaaa atatataaaa tacacctaaa taatgttaca tactatatat
     2401 aaaaatatga taatatgata tatttaatag ggtaaaagta tgtactatat tatacttggc
     2461 aataaaaaat tactttatta aaaggagaga tatttatgtc aatgcttaac aaaaaaacta
     2521 tagaagatat tgatgtgtgc ggaaaaaaag tattagtaag atgtgacttt aatgtgccat
     2581 tacaagatgg tgtaattact gatgaaaata gattgaacgg agctttacca actattcaat
     2641 atttaatttc taaaggtgcg aaagttatat tatgctctca cttaggaaag ccaaaaggag
     2701 aagctaagcc agaattatct ttagcaccag tagcaaagag attatctgag atgttaggaa
     2761 aagaagttgt atttgcagca gacgataatg ttgtaggaga aaatgcaaag aaagcaactg
     2821 aaaaaatgga aaacggagat gtagtattat tagaaaatac tagatataga aaagaagaaa
     2881 ctaaaaacga agagaatttc tctaaagaat tagcatcact tgctgaaata tttgtaaatg
     2941 atgcgtttgg aactgctcat agagcacatt gctcaacagt tggtgcaggt gagttcttac
     3001 aagagagagt ttgtggatac ttaattcaaa aagaattaaa attcttagga gaagctgttg
     3061 caaaccctgt aagaccattt acagctatac ttggaggagc taaagtttct gataaattag
     3121 ctgttataaa cgaattatta gaaaaagttg ataatctaat aataggtgga ggaatggctt
     3181 atacattctt aaaagctcag ggttatgaag taggaacttc tttacttgaa atagataaag
     3241 ttgaatatgc taaagaaatg atggaaaaag ctaagaataa gggtgttaac ttattattac
     3301 cagtagacgt tgttatggca gaccactttg caccagatgc tactccaata gtaacagaag
     3361 atgctaatgt aaaagaagat tacatgggac tagatatggg acctaagact atagcaaact
     3421 ttgtaaaaac tataaaagaa tcaaaaactg tagtatggaa tgggccaatg ggagtatttg
     3481 aatttgaaaa ctttgcaaat ggtacattat cagtagctag agctatggca gagttgactg
     3541 atgcaactac tgtaattggc ggtggagata gtgcagcagc agttaatcaa ttaggatttg
     3601 gtgacaagat gactcacgta tcaacaggtg gaggagcatc actagaattt ttagaaggta
     3661 aagaattacc aggtattgca gcattagata ataaataaac taatatatag aggagattgt
     3721 aaaatgagaa aacctataat tgcaggaaac tggaaaatgc ataaaacaat aaaagaagct
     3781 ttagaatttg taaatgaagt taaagataaa gtaaattctg ataaagtaga agctgttata
     3841 tgtgcaccat ttactttatt aaaggattta aaagaagcta ctaagggtac aaatataaag
     3901 ataggtgctc aaaatatgca ctttgaagaa aaaggagcat ttacaggaga agtttcacct
     3961 cttatgttaa aagaaataga tatggactat gttgtaatag gacattcaga aagaagacaa
     4021 tacttcaatg aaactgatga aactgtaaac aagaaagttt taaaagcatt agaagtagga
     4081 atagacccaa tattatgtgt tggagaaact ttagaacaaa gagaagctgg aaaaactaaa
     4141 gatgtttgta aagttcaagt tgaaaaagct ttagaaaatg ttcttaaaga tgatttagca
     4201 aaagtagttg ttgcatatga gccaatctgg gctattggaa ctggtaaaac tgcaactgct
     4261 gaagatgcta atgacgttat atcttatata agagaagtta taaaaggatt atatggagaa
     4321 ttagctaatg aagttagaat acaatacggt ggaagtgtta aaccttcaaa tgtagccgaa
     4381 ataatgggtc aaagtgatat agatggagct ttagtaggtg gagctagttt agctagtaat
     4441 gattacctag accttgttaa tttctaagga gtgaaatgat gaaaaaacca gttgctttaa
     4501 ttattatgga cgggtttgga tacaataagg atgttaaggg aaatgcaatt gctgagtcca
     4561 aaactcctaa tttagataga ataaaaaaag agtatccaaa cacattgata aatgctagtg
     4621 gtcttgatgt tggtctacct gatggtcaaa tgggaaattc agaagttgga cacactaaca
     4681 taggtgcagg tagaatagta tatcaagatt taactagaat aacaaaatct ataaaagatg
     4741 gtgacttttt tactaataaa gttttatgtg aagcaatgga taatgcaaaa gaaaattctc
     4801 ttcatgtaat gggacttttg tctgatggag gagtacattc acatatagac catcttaagg
     4861 ctataatcaa aatggcaaaa gataaaggcg tacaaaaggt gtatgttcac gcatttactg
     4921 atggtagaga tactgaccct caaagtgcat tagaatatgc caaagaagta caagctagta
     4981 tggatgaaat tggagtaggt gaatttgcta cagtttctgg tagatactac gctatggata
     5041 gagataagag atgggaaaga gtagaacttg catacaatgc catggttaga ggtattggtg
     5101 aaaaagcaaa ttctattgag gaagctattc aaaactctta tgatgatgga aaaaatgatg
     5161 agtttataat gccaacagtt attatgaaag atgataaacc agttggaagt ataaaggaaa
     5221 atgattcaat tatattcttc aactttagac cagatagagc tagacaaata actagagctt
     5281 tagtttgtga agagtttgat ggatttaaaa gagaagatat taaaaacttc tttgtatgct
     5341 taacagagta cgatataact atagagaatg tgcatatagc atttggacct caatctttag
     5401 ccaacactct aggtgagtat ctagctaaaa atggaaaaac tcaacttaga gctgctgaaa
     5461 ctgaaaagta tgcacatgta actttcttct tcaatggtgg agtagaagag ccaaataaag
     5521 gtgaagaaag actattgata ccttctccaa aggttgcaac ttatgattta aaaccagaaa
     5581 tgtcagcata tgaattgaca gataaagctt tagataaatt aggagaagat aagtttgact
     5641 ttatagtatt aaactttgca aatcctgata tggtaggtca tacaggaagt atagaagcgg
     5701 ctataaaggc tgttgaaact gtagatactt gtgtaggaag attgatagat aaaatagtag
     5761 agttaggtgg aagtgcaata ataactgctg accacggaaa tgctgaatat atgttagacc
     5821 cagaaacagg aaaaactgta acggctcact ctattaatcc agtaccattt attgtagttg
     5881 gtcaagaata tgaaagtgca aaattattgg atggaggaag attatctgat atagcaccga
     5941 ctgttttaga tatgatgaag ttagaaaagc cagaagaaat gactggtcat tcacttattt
     6001 caaaatagtc gattctgata agtagagaat aatatataaa ctacaatata aatttaaaaa
     6061 taatgcagtt taggtttatt ttgtaaagtt tagactttat gacagaagat ttagattttg
     6121 taagttttgg atattgtaag gtgtagatga agatatattt tataaaattt acaagataga
     6181 caatttataa aataaaaaat tataatatag ataataatgt aaataa
//
LOCUS       DAMJWU010000154         6221 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMJWU010000154 DAMJWU010000000
VERSION     DAMJWU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.159"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            102..647
                     /locus_tag="PC460_003439"
     CDS             102..647
                     /locus_tag="PC460_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2602288.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            660..1673
                     /locus_tag="PC460_003440"
     CDS             660..1673
                     /locus_tag="PC460_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2602289.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1895..3868
                     /locus_tag="PC460_003441"
     CDS             1895..3868
                     /locus_tag="PC460_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2602290.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3937..4296
                     /locus_tag="PC460_003442"
     CDS             3937..4296
                     /locus_tag="PC460_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602291.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4331..4687
                     /locus_tag="PC460_003443"
     CDS             4331..4687
                     /locus_tag="PC460_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2602292.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4724..6016
                     /locus_tag="PC460_003444"
     CDS             4724..6016
                     /locus_tag="PC460_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2602293.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2459 a          580 c         1181 g         2001 t
ORIGIN      
        1 acaatattag catatagtac agaaaaaaga tgtaatttgt aaaatttatg gtaagcagta
       61 aaattaaaaa tttaaagata ttatagggag gttataattt tatggaaaac gttattacag
      121 gtctaagtaa aggtgcagag tggtttatcg gtttgtttca gaaaggggga gaacaattta
      181 taggtcttgt ttcaggtact cttccaacgc taattgtatt aatggtagct attaactctt
      241 tgattaagat aattggtgag gaaagagtta ataattgggc ttctaagcta ggaaagaact
      301 cttttacaag atatattcta cttccattag tatcagtatt tttcttggga aatcctatgt
      361 gctatacatt tggtagattt ttaaaagaag agcacaaagc aggattttat gatgcagcag
      421 tttcatttgt acatccaatt acaggtcttt ttccacatgc aaatgcagga gaactttttg
      481 tatggttggg aatatctgca ggtttgacta ctttaggtaa agaaactact acattggcat
      541 tgtggtattt tatagttggt ctaattgtaa tttttataag aggtatagta acagaaaaaa
      601 tgtatgcttt tctaacaaga aaaaatagag caacaaataa ggcttaggag gtagataaaa
      661 tggaggagaa taaaatatta aaaatagaaa agggaacttc aggctgggga ggtcctttgt
      721 atataaaaaa agaagggaat agaaataaga tactgtcaat gactgctggt ggtatccatg
      781 aagtaacatt aaaaataaaa gaactactgg gttgtgaaat tgtagatgga tttaaaactg
      841 gagtttctga tgaagaagta gcagtagtta ttattgattg tggtggaact gctagatgtg
      901 gagtttatcc aaagaaaaaa atacctacaa taaatgtaaa tccagttgga aaaacaggtc
      961 ctttagcaaa atttataaca gaagaatatt atgtatcaga tgttaatcct aactgtataa
     1021 gtgttgtaga tggggaagat atgccacaaa aatctcaaga aaataaaagc gaaaataaga
     1081 gttctattag aaagcctgat aattatgatg aggttaagtc aaaagcacaa ggtgaatatg
     1141 ctaaaaagaa cataatactt tctattggtc aaggggcagg gcaagtagta tcaaaatttt
     1201 atgatgcagg tcgagatact atccaaatgg taatgaataa cgtaatacca tttatggcat
     1261 ttgtaagtat gcttatggga attattttag catctggact gggagactgg attgcaagag
     1321 ttatatctcc acttgcaggt aatattggtg gattattgat tatatcagta atttgcacac
     1381 ttccattctt atcaccaata ctaggacctg gtgcagttat agctcaagta gtagggactc
     1441 ttgtaggtac acaaattggt cttggtgcaa taccagcata tttagcactt ccagctttat
     1501 ttgcaataaa tgggcaagct ggatgtgatt ttgtaccagt aggattaagt ttgggagaag
     1561 cagaaccaga aactgtagag tatggtgttc cagctttatt ttactctcgt ttaataactg
     1621 gtccaatagc tgttattatt gcatatggag ttgcagtatt tgcattgaga taatatttaa
     1681 tgtcaatata aggcttggaa ttaattaata aaattgtaaa tataaattta agctagtcaa
     1741 gattttttgt taatgaaaga ctagctttta tactattaat tagctgtaat tatcgagtta
     1801 gattaatact aaattttcct ttattaaagt gaaattttat gtattaattt ggtttcaaat
     1861 ttgtactaga atgaaatcaa ggaggagata caaaatgagt gtaactgaga aaattccttc
     1921 tattgaccgc aagatattta agatattgat tatgtgtagt aaaaaagagt ttgtgagtat
     1981 taactcaata gcaaatgaat tgaatgtcac aactagaagt gtaaggacgt atataaaaca
     2041 attgaataaa gatttaggta atgatatagc agtaatcaaa tatataaagg gtcaaggcta
     2101 taaactagaa ataaaagatg agcagatact caataagatt attgatatga atagaaaaaa
     2161 tgtattttca ttaaattcaa aagaagatag agtagagttt atactaaatt acttgataga
     2221 actagatgga tttattacac ttgatagttt agctgatgag atgtgtgtag gaagaactac
     2281 tttagtaaat gatttccagt atgtagaaaa agttttggct tcatataatt taaacttgat
     2341 taaaaaacag aatacaggta tgaaattaaa tggaaatgaa cttgatatta gactgtttat
     2401 attaaatcag ttgtataaaa actcaaggaa agatttcagt aattcaaagt attttaaagg
     2461 aatcaaaaaa gaagaaatca taaatttaga agaaaaacta ttgactttat ttaaaaaaaa
     2521 taatttttat gttacagatg aaatgttaag agaagttatt aactacatag tagttttagt
     2581 ctatagagta aaagaagaaa agaaagtaaa agattatgat gtaaagtttg atttacttaa
     2641 atcatatgat gaatacttta ttgctaggga aataaaacat ataatatcag gaatgtttga
     2701 atgtacttta aatgatgaag aaattattta tttaactata ccgttagtta gtggaaatgc
     2761 accagcatca gaatgtgctt taaatagtag tagaattagt aaaaacattg atgagttgat
     2821 ggaaaatata tttaatcaaa tatatgtcga tatgggtatt tttataaatg aggatgaatt
     2881 aagagtaggt ctaggatatc atcttagttt tactttaaac aggctattat ttaatataaa
     2941 gttaaaaaat gttttgttag aagagattaa gcaaaattat atacttccat ttaaattagc
     3001 acaaatagca ggaaaagtaa tagagaaaaa gtataactta gaagtttcag aagatgagat
     3061 tggatatata gcaatccatt tcagtggata tttagaaaga aatagcagta gattctattc
     3121 aataaaaaaa atagcaatta tatgtagtac aggtctagga acagcaaaac tattgaaaat
     3181 aagagtagaa aaactgatag gaaataatcc taaaattgat actctttcaa gttttaattt
     3241 gagaaatatt aacttagatg aatatgatat agtttttaca actattgatt tagacacatc
     3301 aaatataaat acaatagtat taaaaataaa tacaatattt gatgaaaaca aactaagaga
     3361 acaattaaaa acagtattgt gcctaagaga agggaatatt gacactacta attctacaaa
     3421 tttactaata aataatttgt tagatgaaga taaatttatg atattaaatg aaaagactat
     3481 acttgcttca ttagaaaaaa tgatggacca tcttatggat ttaggatgca ttgatgataa
     3541 gtttagaaaa aatatagtca atagagaaga aaaatctccc acagtatttg ataaaggttt
     3601 attatttcct cattctgtaa atgaaaaatc agataagttt ttaatggcag ttggaatttt
     3661 agaagaacca atagaatatg caaatagaag tataaaaata attcttatga caatgtttcc
     3721 atgtgagaat aagatagact cagacttatt agtaaaaatt tatgaagaag ttttaaagat
     3781 aggccaagat gtaaaattaa cgaataagat aagtaaatgt agaacttttt tagaatttaa
     3841 aaaggtgtta cttaaaaacc ttatataact attgtacagc atttatagtt gttatacaga
     3901 tttaaaaata atgtgatttt tggaaggtgt ataaatatga aattattagt gattttattt
     3961 gttgcttata ttttaaatac tatatttata atgaagcaga ataaattgta ttttaaaact
     4021 ttaaatagag ctaaaaaaaa gggtgatata gtgtctacag gcaaaaagaa aagctatttt
     4081 tctaaaggaa gtatagccat tatatcttca gattcagatg ggtttataaa gtttggagaa
     4141 attttaaaag gacgtacagt aatggctaaa tttaaagaga ttgaagaaat agaaggatta
     4201 gatatatata gtgcagagca aaaatttaaa gatgaagaga gtattgtaca agctataaaa
     4261 tttataaaag aaaaaataaa tgtttctttt aattaactca atataaatat aaattatcag
     4321 gaggaatgtc atgaaatatg aagttaagat aagtgggata ggcagtttag ttaaagagtt
     4381 aatggatgaa agcaattgtc ttattatata tgatgaaacc attaatgatg acgacttaaa
     4441 ggatatatct gtaattcatt caatagcaac gttaaaaagt gatgttgaaa taggagatac
     4501 acttactata gggaatagag attattgtat agtttcagtt ggtgatattg ctcagaagac
     4561 tcttagagaa attgggcatt gtacaatcaa atttgatgga aaatgtgaag taaatttacc
     4621 aggagaaata catgtggaaa taggaaatcc agatataact attggagatt taataactat
     4681 ttcttaattt gaaaataaaa ttattataac aggaggaatt ggtatgttag atttaaacag
     4741 aaaacttgca aaattggaag aggaaggtaa aaaaataaaa gtagctctta taggagctgg
     4801 gcatatggga aatggaatgg taagccaaat ggcaaatatg aaaggtattg aagcttctat
     4861 tgttgtagat ataaatttgg agttggctca taaagctttt actgatgcag gaatagatga
     4921 agaaattata gataatgtaa caaatgaaaa ggatgcagaa ataaaattac aagaaggaaa
     4981 agtattgaca tgtaatgatt ttcttgtggc ttgtaaaact aagtcaatag atgtagttat
     5041 agatgcaact ggaggtattg caattggtgc agagatagca ttaaactcta ttttaaataa
     5101 aaaacatata gttatgttaa atgctgaaac tgattgtgtt gttggaccaa tacttaaaaa
     5161 attagcagat gatgctggag taatatttac aggttctgca ggagatgagc caggtgcagt
     5221 aatggaatta tttgattttg cagatgcaat ggggtttgaa gttagagtga taggtaaagg
     5281 taaaaataat aaattagatt tagattgtaa tcctgataca gttagagaag aagcagaaag
     5341 aaaaggagct tcacctcata tgatagcttc ttttaaagaa ggaactaaaa caatggtaga
     5401 aatggcttta atgtgtaatg caactggatt tgtgcctgat gtaagaggtg gtcatggtat
     5461 tgaagctaca gttaatgaag tacctaagaa atatgcatta aaaagcgaag gtggagtatt
     5521 agataattat ggtgtagttg attttgtaaa tggtatagca cctggagttt tcgtagtagt
     5581 agcccataaa ttaaaagcag ttaatgatga actaaaatat ttaagtatgg gtgatggacc
     5641 taattacata ttatatagac catatcattt atgtagttta gagactcctt tatcagcagc
     5701 tatggcagtc ttagaccata aggctacaat agtacctaaa gctggactag tagcagaagt
     5761 tatgactata gctaagaaag atttgaaaaa aggtgagttt atggatggct atggagcata
     5821 tacatgctat ggtactatag aaaaatacga tgtagcaaag gctatgaatg ctgtacctat
     5881 tggtttgata agtaaaaaga ctaaagttgt aaaagatatt aaaaaaggtg aagttataac
     5941 ttacgatatg gtagagatag aaaaagatac tactttatat catttaagac aattgcaaga
     6001 aaaaatattt gggtaagatt tgatggtaat ataaatagat attagtaaaa agggttgatg
     6061 gttattttta aatcaaccct tttaaatttt tatatattct tgttaggata ctttctttaa
     6121 aagtatatta taggttattt gtagtcgtaa aaattatttt gaattagttc tgcaatttta
     6181 tattcacatt gaaataatat tattaaaaat tttaatataa a
//
LOCUS       DAMJWU010000155         6191 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMJWU010000155 DAMJWU010000000
VERSION     DAMJWU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.160"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            355..921
                     /locus_tag="PC460_003445"
     CDS             355..921
                     /locus_tag="PC460_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2602294.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            961..1542
                     /locus_tag="PC460_003446"
     CDS             961..1542
                     /locus_tag="PC460_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2602295.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1599..2516)
                     /locus_tag="PC460_003447"
     CDS             complement(1599..2516)
                     /locus_tag="PC460_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2602296.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2646..4277
                     /locus_tag="PC460_003448"
     CDS             2646..4277
                     /locus_tag="PC460_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2602297.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFVRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4280..5227
                     /locus_tag="PC460_003449"
     CDS             4280..5227
                     /locus_tag="PC460_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF2602298.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5512..5775)
                     /locus_tag="PC460_003450"
     CDS             complement(5512..5775)
                     /locus_tag="PC460_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF2602299.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5874..6119)
                     /locus_tag="PC460_003451"
     CDS             complement(5874..6119)
                     /locus_tag="PC460_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602300.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
BASE COUNT         2243 a          836 c         1000 g         2112 t
ORIGIN      
        1 ataattgttg tttttaagat aaaaattaaa tgtatcaatt attgttttat acaagtattt
       61 ccaaatccgt tctcatttag atagtatgct tagaaatttt agaattattg agtctaactt
      121 tattacttac tttactttat aacaatcaaa tacaaattta aatattataa aactacaaaa
      181 taatttaaag catataatgt caatatgttt aaattataaa atattcatgt agttatataa
      241 taaataattt ataaatatag ccaacttagc ctaaatacgt atataatata gctaatatgg
      301 ttcttatact tgataccatc aataaaacta aaaatataaa aaggagaact taaaatgagt
      361 gttagtttac aaaaaggaca aagagttagc ttagtaaaag ataataatcc tgtaaaaaat
      421 ctagtagtag gcttaggatg ggatatgaat aaattgggta aaaaaaatta tgacctagat
      481 gcatttgcaa tagcattgac taaccaagat aaaatgaatg ttagaggtga cttggtttat
      541 tttggaaatc ttaaacaccc ttctaaagct attatacaca ctggagataa cttaactgga
      601 aaaggtgaag gtgatgatga acaaataatt gttaatttag aagacatacc tgaatatgta
      661 cataaaatag tttttggagt ttctatatac aaagctaaaa aaagagacca ggattttggt
      721 caaatgaaca actcttttat aagattaatt gattcaaatt ccaagcaaga attatttaaa
      781 tataatcttc aagaagactt ttctggtaaa gtaacagttt tggcaggaga aatttatcgt
      841 agaaatgaag aatggaaatt taatgcttta ggaattggtc aaaatgaaga gcttcgtgaa
      901 ttaattaata catataagta ataattacaa taaaaaattt ataatgagga gttggtttaa
      961 atgggtataa ctttagcaaa aggacaaaaa gtaagtttaa caaaaagtaa ccctggtcta
     1021 aaaaaagtaa tagtaggact aggatgggat attaataaat atgatggtgg ctttgatttc
     1081 gatttagatg cctctgcatt tttaactgga actgatggaa aagtaacgaa tgatggagac
     1141 tttatattct ataataattt aaaacacgct tctggagctg ttgaatatat gggtgataat
     1201 agaacaggtg ttggtgatgg tgatgatgaa caaataaatg tagacttatc taaaatacct
     1261 caaaatattg ctaaaatatc atttagtgtt actataaacg aagcaattac aagaagacaa
     1321 aatttcggac aagttgaaaa ttcatatata agaatatata atgaagaaac taatgaggaa
     1381 cttattaaat atgaattggg tgaagatttt agcatagaaa cagcaatagt tgtggcagaa
     1441 ctatatagac ataatggaga atggaaattt aatgctttag gttctggatt tgaggatgga
     1501 cttgcaggat tgtgtaaaaa ttttggcgtt aatataggct aatatatcta aataaaaata
     1561 agagtatatt gatatactct tatttttatt tagatatatt ataaactgtt tacacttcta
     1621 ctcttctttc tcgcttgttt tttcccatat tctactgaaa tatcaaaaaa tgctgaaata
     1681 ttttctgtaa aaaacttgct tctatgatat gcaatatcaa atgtcctatg taaattaata
     1741 ccttcaatat cacaagccca caaaagatgc ttttttaaat aatcctcaat aagtaattcg
     1801 gatataactg caatgccaaa attatctaca actgctctta atatagcttc agagttataa
     1861 catgaccatc taatattcat tgggattttt aattctttta attgcttttc taattgtgct
     1921 cttgtaccac ttcctaattc acgcataatt aatggttgat ttgaaagttc tgaaactttt
     1981 atagagtctc ttttataaaa ctcatgcttg tgtgaacata taagtacaag attatcatta
     2041 ataatgcttt ttgtaacaat atctgggtgc ttaatttttc cttctacaat cgcaatatct
     2101 aattcactat taagtaattt ttttgcaatc agtcttgtat cttctacaaa tacttcaggt
     2161 tctataagag gatttacctt gtataattct aacattattg gacttattac acatgtacca
     2221 acagttactg ttgccccaac tcttatacgg atattttttg cacaatgctt catacaatca
     2281 ttcatttctt catttacagc taaaagatgc ctcccatatc caagaagttt tttccctgcc
     2341 tcagttaaat ataatttttt tgaaattcta tcaaatagct taacaccata ctctttttct
     2401 atttctgaaa tagcttggct aactgatgac tgggtaatat acatatttct tgctacttca
     2461 ctcattttac cacattcagc aaccttaaca aagatttcaa gactacgaat tgtcatatta
     2521 tcacaacctt agcaaacatt agtaaatgct tatgatttta ttaatatttt agtattttac
     2581 aaatatattg tcaagaagta taattttatt atagagttat aaagtagatt ggagtgtgat
     2641 atattgtgaa agtattaatt ataggtggag ttgctgctgg aacaaaaact gctgcaaaaa
     2701 taaaacgtga aatgggtgat aattgctctg ttacaatact aaataaaggt gatgatattt
     2761 cttatgcagg ttgtggtctt ccatattacg ttggtaagat aattgaagat aaatcatctt
     2821 taatcgtaaa tacacctgaa agtttttcta aactaacagg agcagaagtt cgttgtggag
     2881 ttgaagttac atctattgat agaaatgcaa aacaagtaac tttgaaaacg ctttcaacag
     2941 gagaaaaaga aaatctaagc tatgacaaac ttgtaattgc tacaggggca gacccaataa
     3001 aaccaccaat acctggaata gatttacctg gagtattctt tgtgcgtact ccaaatgatg
     3061 ctataaaact aagagattct attgaaaacg gtattaagag agctgttgtt attggtggag
     3121 gtttcattgg tcttgaaatt gctgaaaatc tttctgcaat gggtatacgt gtaagtgttg
     3181 ttgatatggc tgaacatgtt atgcctggat ttgatactga ttttgctgaa tacgttgaaa
     3241 atcatatggc tgataaaggc ataatgattt ttacaggtga ccaggttgta ggtatcgaag
     3301 gtgaaaataa agttgaaaaa cttcgtacta aaaatagagc tattaaaaca gatttagtgg
     3361 ttatgtcagt tggaatccgt cctaacacag gttttctaaa agacacaggt ttagaatttg
     3421 cccctaataa tacaattatt gcagatgaat atatgcttac aaatgacaaa gatatatacg
     3481 tagttggaga ctgtgctttt gtaaaaaatt ctttaacaaa taaacctaca tggtcaccta
     3541 tggggtcatc tgcaaatcat gagggtagaa tttgcgcaca aaatatatct ggaaaatcta
     3601 aaacttacaa tggtgttctt ggaacaacag tagtaaaatt acctgagctc aatgcaggaa
     3661 aaacaggtct aagtaaagaa atagctgaaa aagaaggtta taatgtttgc tctgtaacca
     3721 tagctactga tgataaagct cattattatc caggtgcatc taattttatt ataagaatgg
     3781 ttgctgaaaa aaatactaga aaattattag gtgttcaagt tatgggacct ggagcggttg
     3841 ataaaatagt agatatcgct gctactgcaa taactttagg cgctgacctt tattcattag
     3901 aaagtatgga ccttgcatat gcaccacctt tttcaacagc tattcaccct tttgtagtgg
     3961 cagtaaatgt tttacaaaat aaacttaatg gagaacttga tagtgtttta cttgacgaaa
     4021 ttgaggattt tgacagttgg actaaacttg atgtttcaaa agctccttct attcctagtt
     4081 tacgttatat tcctgttggt gaaattaatg gagaaattga aggccttgct aaggatgaaa
     4141 aaattcttct tatatgtgct aaaggtaaaa atagctatat ggcgcaaaat cgcttaagac
     4201 gctttggcta tacaaatacg aaagttcttg aaggtggaat actattttat ccaaatctta
     4261 aggtaatgga ggagtaaata tgagtaatgt tacagcagaa caaaaaaagg aagtaaaagg
     4321 tagaggtttt ctaccaagca gggatggaga acattttgca gcacgtataa taactgtaaa
     4381 tggtgtaata aatgcatcac aagcaaaaaa aattgcagaa gctgctgaaa aattcggaaa
     4441 cggtcagtta gcatttacta ctagactaac agtagaatta cctggtgtaa aatttgaaga
     4501 tattgaagct ctaagtgaat ttattgctag cgaaaatcta ataacaggtg gaactggatc
     4561 aagggttcgt cctgttgtgg cttgtaaagg tactgtttgt gttcatggtc tagctgatac
     4621 acaggcactt gctgctgaaa tacacgaaga attttacaaa aattggtatg atgtaaaact
     4681 acctcataag ttcaaaattg gtgttggtgg atgccctaat aactgtatta aacctgacct
     4741 aaacgatctt ggaattgttg gtcaacgtgt acctgactat gattcagagc tttgtgttgg
     4801 ttgtaaaaaa tgtgctgttg tagaggtctg tcctgttaaa gcagctaaat taacagataa
     4861 aggtaaactt gaaatagata gtaatctatg taataactgt ggtaagtgta tagaaagttg
     4921 taattttgat agtattgaag aaaaagaatc tggttataaa gtctatatag gagggaaatg
     4981 gggaaaatca gttcgtcctg gaacacagat tgatagatta ttttcaaaag aggaacttat
     5041 gactttaatt gaaaaagcaa ttcttttatt tagagaacag ggtaacacag gtgaacgttt
     5101 tggaataact atcgatagaa tcggtgctga taaatttata gaaatgcttt tatcagatga
     5161 agttttggaa agaaaagaag agatactaga ggctctttta caccttacag gtggagctgt
     5221 atgctaacaa taaatatttt aattgtacag ataacctatg gaaaaaattt tagttaagaa
     5281 taattaaaat tctatatatg atatatgata aatgaggcta tctcaaaaca cagatacgcc
     5341 tttttttcat tgttgaaatg ataaagtata tttgagttca tataaaaaag agtgtctcgt
     5401 gaattataaa attcattttg aggcacccac attattttat aataattgct tattaatttt
     5461 attttaacta tataaaaagt acaaaaagta agaatatcca atattctaaa attaattttg
     5521 aattgtattg attgaaaagt ttactattcc actatttcca tcttgattta ctactagata
     5581 ccatgttcca gtattaggaa ctctcattct acatggattt ttctttgcaa atccaccata
     5641 gtaatcataa tttctttgat ttttaaattt cacataattt atatggtcta acaatattac
     5701 attggcttgt ttctctaaat ttacctctac aatatcccct ttttttaagt tcatcttact
     5761 ataactatat tccattgaat tactcctcca gtatcgaata atctttgtta tttactcatc
     5821 tcaaatatgt aaaaattcat ttaaaatatt acaaatacta tataattaga ctattatcca
     5881 gttttaacct tatttattac aatccaaagg ttattaaaaa tattattggc aacaacttct
     5941 ccatcccata caactctttc ttctttattt tctaataaag catagtaccc agagtcagaa
     6001 ctttcatctt tatagactgt aacataaaac tcttcatcac tatattcagg tatattttta
     6061 aagtcttcag atattttaca tgttaatata taaccttcta attcttcatc tataaacaaa
     6121 gcaattcctc caatctctat atttttacta attataccat attatgtcat aaacttttta
     6181 ttttattaca a
//
LOCUS       DAMJWU010000156         6164 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMJWU010000156 DAMJWU010000000
VERSION     DAMJWU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.161"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            287..1654
                     /locus_tag="PC460_003452"
     CDS             287..1654
                     /locus_tag="PC460_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF2602301.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1679..2287
                     /locus_tag="PC460_003453"
     CDS             1679..2287
                     /locus_tag="PC460_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF2602302.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            2335..2706
                     /locus_tag="PC460_003454"
     CDS             2335..2706
                     /locus_tag="PC460_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF2602303.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2885..3223
                     /locus_tag="PC460_003455"
     CDS             2885..3223
                     /locus_tag="PC460_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF2602304.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            3689..4192
                     /locus_tag="PC460_003456"
     CDS             3689..4192
                     /locus_tag="PC460_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF2602305.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            4239..5198
                     /gene="moaA"
                     /locus_tag="PC460_003457"
     CDS             4239..5198
                     /gene="moaA"
                     /locus_tag="PC460_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF2602306.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            5202..5672
                     /gene="moaC"
                     /locus_tag="PC460_003458"
     CDS             5202..5672
                     /gene="moaC"
                     /locus_tag="PC460_003458"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF2602307.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            5708..6139
                     /locus_tag="PC460_003459"
     CDS             5708..6139
                     /locus_tag="PC460_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF2602308.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         2607 a          504 c         1117 g         1936 t
ORIGIN      
        1 aatttttata tagtaaatga caaaagttta caataaatgt atattttctt actcattcct
       61 taaaattact catttagttt aataactctc atttcatatg taaagttggt aaaaaaatta
      121 aaattaaaag taaaaaacta aataaattaa tgttaatttg aaatatatat gaaggttttt
      181 ggagaatact tacttatgat aggaaaaaat gttaaaataa tagtgtaatc aaatataaag
      241 gactgaaata attaacagtt attaaatatt aggagtgtaa aaatttatgg gattaaaaga
      301 atttgatttt attgaagagt ctattgatat gttgaggaca atgtcaccaa cattagaaac
      361 tatatcagat gaaatagaag agtattttga aaatatcttg gatgaaaaga atcaagaata
      421 tataaatgta acctctagaa taaaatcaga atcaagttta agagaaaaga taattagaaa
      481 tagatattta aaaaaatatg gagaagcaag taatcttatt cataatgttt ctgacttaat
      541 aggcctaaga attgagtgta gatttattga ggatgaaaac aaaatatata gacttttgag
      601 aagatatttt aataaaacag atgacaaaat aaactattat aataaagaga ataagaatat
      661 taaattaaaa ctatctgaga gacaacccaa taagcaaaaa aatggatttg aaatatataa
      721 aatagatgga gtttttgcgt atttagatag agaagtaaaa tttgaacttc aaattaaatc
      781 tcttgtaaat gtgttttgga gtgaaataga acataaaata atttataaaa acaatacata
      841 tttacttgct gataaattta taaaggatat gatggactct ataaagaata atcttactat
      901 gatagataat cagcttttaa gtatttataa aaactttcat tcagggaagt cttttaatat
      961 gaaagtaagt aaaaaagaaa ttgaaaaatt atttgcgaaa ctagtctatg acgctttttc
     1021 tgaaaagatg aataaaagta taggttttgt tgtagatttt aaaaaaccat gtgagactat
     1081 attgaactat tcttttaata aacaagatat aagtgatgaa gttttaggta attttatgct
     1141 tgatgagttt gcaagattaa atgaaattgt aaacaaggat atagatttta atgagcaaat
     1201 agaatttgaa agagaaccaa catttgatga taagttttgt aaagacctag gaaatcattt
     1261 tagaagtaga ttaaacacag aatttccatg gaacctattt tttagaatat tatttgaaat
     1321 agagccatat aacaatacca aagactttga aaactttgta gagtttataa aagaaaatgt
     1381 gctactagaa gaaaataggg agcaattatt atgccaattt gaagaagata gcaaacttat
     1441 aattgaagat atatatgatt gtatatttaa tagtataagt gagattgata gtgttgaaat
     1501 tttatataac tataatcttg aaaaaataaa ctttacatca agtgaaatca taaattgtat
     1561 atgtagagaa tatgagtgtt atggtgaata cttagaagaa aaagaaaagc ttatgaatac
     1621 atttaaagaa aagattattc aaatctttga gtaataggtc aatgctgaag tggcgctaat
     1681 gcgccatttt tcaaatatat atgaattaag taaaattatt ttggaggagg cgatttgtat
     1741 agagaaaaca ggtgtaattt tggcaggggg aagaaattct agaatgggta gagataaagc
     1801 ctttttagag ttacatgata agctttttat tgaaattgca atagaagcat ttaaaaactt
     1861 tgatgagttg ataataatat ctaataatga agaattatat tctaaatacg atattaaagt
     1921 ttataatgat atagtaaaag atgttggtcc aattggagga atatatactg ctcttgaata
     1981 tgcaaagtat gatatagtta caattgcatg tgatatgcca tatctaaata atcaaatagt
     2041 cgaaagaata gctaataaaa tgaatgataa aagtgtgatt tcagtgacaa atggaaagtt
     2101 acaaccattg tgttctggat ataaaaagag tataataaat aaagtatctt tatgtataag
     2161 ggaaaatgat ttaaagctca gaagctttat agataaaata gataaaagtt atatatactt
     2221 tgatgaagaa gatttatttt taaatgtaaa cactgttgat gagtatgaaa aattaactaa
     2281 agtttaatta gctatggttg taaaatagag taggatttat atggaggaga gtttatgaat
     2341 ttacttaaat gcgattcttg ggcagtaata ttaaataata gcgataaaga aagtaaagca
     2401 tataaaatat tagatgaact taaaaggaat atgtacaaag ttgttgctat agatgaagag
     2461 aaaaaaccta ttgaaggaat agatgtgtat gaatgtttaa aagatgttcc acacaatatt
     2521 gatgttgtag ctattattga taaacaatct aagatggatg tgattttaga agaagtagag
     2581 cttttggata tacaaaatat gtggtttgaa aaaggaagct ttagtgaaat gatgattaaa
     2641 aagactaaag atttaaaatt aaatatagaa tataatttaa gtttatatga tgagttgact
     2701 agataaaatt atatatctta ataataaaaa tataattagt taataaaaca tattatttta
     2761 tagtatagat tggttaataa aagtacagat tggttaatga aacattaaat tacaatattt
     2821 gttaaaaaaa ttgtgagtta catgatataa tgtattaaat aggatactaa ggagttgata
     2881 tattatgtta atagtaacta cagaaaaagt agaaggtaaa aagatatcaa aggttttagg
     2941 attagtgaga ggaagtacaa taagagcaaa acatgttgga aaagatatag gagcaagttt
     3001 taagaatctt gttggaggag aacttactgg atataatgaa atgctcactg aagcaagaca
     3061 aattgccata ggtagaatgg ttgaagatgc agaagctaaa ggtgcaaacg cagtaatagc
     3121 atttagactg tcttcagctt cagttatgca aggggcagca gaaatgcttg cttatggaac
     3181 agcagttgtt ttagaagatg ataatagtat tcttgaaaaa taaaatataa atagttaaaa
     3241 agaggcatct tttaaaaatt ttatggatgc ctttttttat ttgaaaaaac taatttgaga
     3301 agaaatttta actattatga caataaaatt agggtgatat tatataaata agtaaaatta
     3361 tattatataa tgtatggtat agactttaaa aattttattt gtagagaaaa atatggacaa
     3421 tgttataata attatagaaa ttaaaagtac ttacattcat tatgtatgtg acatgtataa
     3481 ttagttttat atttaataca gttttgaagt aatatcttca ttttgagaac gttggaataa
     3541 taaaaaatgt attattatag gtgatataaa taaaaaataa atataatatg ttatattgct
     3601 gatagaaaag agaataaaag aaaataggtt ttattaaaag agatttttat ttataggact
     3661 taatcaatgt atagaggggt gagaacatat gtttaatgtt gcaattataa cacttagtga
     3721 taaaggatat gaaggaaaaa gagaagatat aacaggaaaa aagttaactg aatttgttga
     3781 aaatacagga gcttataaag ttactgaata tgtacttata aaagatgata aggaaatgct
     3841 aaaggaaaat attataagac tttgtaatag taataaaata gatttaattc ttacaaatgg
     3901 aggtactggt ttttcaaaaa gagatataac cccagaagca actaaggaag tgttggaaaa
     3961 agaaatacca ggtctgagcg aatatatgag aatgaaatcc acagaaatta ctaaaaaggc
     4021 catactaagt aggggtgtaa gtggaataag aaataattct ataataataa atttacctgg
     4081 aagtcctaaa ggtgcagttg aaaatctaag ttttataatt gatgttttag accatggcat
     4141 agaagtttta agaggagaag ctacagaatg tgccactaaa aaaagtgaat agtatataat
     4201 ctagatagat aaaaaataga caaagaggta gataaagtat gattgataag tatggaagaa
     4261 aaatagacta ccttcgtatt tcacttacag ataagtgtaa tttaaggtgt gtttattgta
     4321 tgccaccaga tgtaaaattt gataaaaatt atataaatga aaatctaagc tttgaagact
     4381 acaagtttat tataaaggct atggctgaac aaggtataac aaaaattaga tttactggag
     4441 gagaacctct tttatatcct aaattaaatg agcttataaa atttacaaaa gaagaatgtg
     4501 gaataaataa cataggaatg accactaatg ctattgggct ttctgataga atctttgagc
     4561 ttgaaaaatg tggcttaaat aaagttaata taagtttaga ttctttgaag gaatataagt
     4621 ataaatccgt aacaagagga ggaaacttaa aagatgtctt aaaatcgatt gaatcttgtt
     4681 taaacttagg tataaaagta aagttaaatt gcgttgctat aagtgggttt aatgatgatg
     4741 agcttaaaga ttttatgttt ttaacagtta aatcaccaat tgatgtaaga tttatagagc
     4801 ttatgccatt aggtgaggct aatagagttt ataagaaggg ttattttaat gtaaaagaaa
     4861 tgattgaaaa aatatatggt ttatataaag tcaaaaatga tgaaaaaagt acagcagaat
     4921 attatatgtt taaaggagct aaaggaagaa taggaattat aactccatta agctgttcat
     4981 tttgtaacac ttgtaatcgt attagactta cttctagtgg tgcaataaaa ttatgtctac
     5041 attcaaaaga agaaatagat ataaaaccat ttttacataa accattgtta tttagagaat
     5101 ctatgaaaga tataataaaa caaaaaccag aaaaacatca tttaatagaa aataaatgta
     5161 gtgatacaaa taggcgtatg tatgaaattg gaggataagt aatgttaaca catataaatg
     5221 aaaaagggta tgccaagatg gtggacataa gtgaaaaaga tgatactaaa agaacggccg
     5281 tagctacagc tacaattact cttagtaagg aagcattaga aaaagtaaaa aatggtcaga
     5341 taaaaaaagg agacgtattg tctgtagctc aagttgcagg tataatggga gctaaaaata
     5401 catcttcaaa cataccaatg tgccatcaga ttaaattagt tggatgtgat ttagaatttg
     5461 atgtagatga ttctatgtct gctattaaga tatacgcaac ctgtaaatct cttgggaaaa
     5521 ctggagtaga aatggaagct ttaaatgcat gttctttagc agcacttaca atttatgata
     5581 tgtgtaaagc agttgataaa aaaatggtta taagtaatat ccatcttgta gagaagactg
     5641 gtggaaaatc aggagatttt aagttcgagt aaattaataa tatattgggt taaaatttgg
     5701 aggaaatatg gcgaaggtag tgtcaataaa tataagtgag aaaaaaggtg taattaaagt
     5761 tcctgtacaa tgtgcagagc taaaaataaa tcatgggata gttggagatg cacatgcagg
     5821 aaactggcat cgtcagataa gcttacttgg aaaagaaagc atagataaaa tgaaaatgaa
     5881 aggctttgac caattaaaat atggagattt tgctgaaaat gtaactacag aagggataga
     5941 agttttttcc ctgcctgttg gtacaaagtt aagaataggg caatgtgaag ttgaagtaac
     6001 tcaaataggg aaaaaatgtc acaatggatg cgaaattaag aaattgacag gtgactgtgt
     6061 gatgccacga gaaggaattt ttgtaaaggt tctaaaagaa ggaattataa atgtgaatga
     6121 ttcaatagat gtaatctaat gaagtcacta ttaaaatata atta
//
LOCUS       DAMJWU010000157         6140 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMJWU010000157 DAMJWU010000000
VERSION     DAMJWU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.162"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(81..242)
                     /locus_tag="PC460_003460"
     CDS             complement(81..242)
                     /locus_tag="PC460_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602309.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(583..1023)
                     /locus_tag="PC460_003461"
     CDS             complement(583..1023)
                     /locus_tag="PC460_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2602310.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1095..1565)
                     /locus_tag="PC460_003462"
     CDS             complement(1095..1565)
                     /locus_tag="PC460_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2602311.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(1582..2892)
                     /locus_tag="PC460_003463"
     CDS             complement(1582..2892)
                     /locus_tag="PC460_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2602312.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDIGLVESSLIYWAAGVIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVN"
     gene            complement(2894..3070)
                     /locus_tag="PC460_003464"
     CDS             complement(2894..3070)
                     /locus_tag="PC460_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602313.1"
                     /translation="MSKTLSKGTDYKFTKEQIVNSKKYINRKDLLNAILKENELYSFS
                     EVEEIINSFMKGVS"
     gene            complement(3063..3500)
                     /locus_tag="PC460_003465"
     CDS             complement(3063..3500)
                     /locus_tag="PC460_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602314.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEIDEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKNNDINKMDTVELFGGLKRGDNFE"
     gene            complement(3849..4655)
                     /locus_tag="PC460_003466"
     CDS             complement(3849..4655)
                     /locus_tag="PC460_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602315.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNNLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(4765..5055)
                     /locus_tag="PC460_003467"
     CDS             complement(4765..5055)
                     /locus_tag="PC460_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2602316.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(5055..5402)
                     /locus_tag="PC460_003468"
     CDS             complement(5055..5402)
                     /locus_tag="PC460_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602317.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(5396..5797)
                     /locus_tag="PC460_003469"
     CDS             complement(5396..5797)
                     /locus_tag="PC460_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602318.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(5807..6040)
                     /locus_tag="PC460_003470"
     CDS             complement(5807..6040)
                     /locus_tag="PC460_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602319.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
BASE COUNT         1818 a         1229 c          590 g         2503 t
ORIGIN      
        1 ttttctaatt tgtggtataa taaaagcaag aagaactaca atctattttg cggtagagtg
       61 aagttcataa ttaaatgaat ctatttgaac ttatggaact tgattttaaa atcaaattcc
      121 cagccacttt tactcttgcc acgagttgag tggcttttta cttttagaaa tactttacaa
      181 attaagcaaa atattaaact agcaataacg ccagctatta cattaagtaa aaagttattc
      241 atacttccca cctcctttca ttaggaagta ggttttatcc cagtatgaac tccactctat
      301 aaattgtaga ttacatcttc ttgctaaaaa tattataaca tataattatt acatatttta
      361 cctattctat atttattttt ttattttgct atcttcttcg tcccctcttt ctctctcttt
      421 cagcttcttt cattgcttcc tcttcatctt ctatctttat aagtattgag gcggctgcta
      481 acgctctctc attaacttct aaattcatat attcacttgg cttccacttt aatttttgaa
      541 tacaataatg agtgatgcta gcatcaaaat cgccacctct gattagtttt ttgcttcttc
      601 tactttatcc tcaaaagatg tatcaaatcc attgacttca ttcactttta ctgtataatt
      661 gacatactca cctgctgtaa gcattgtctt taataactga gcttctccca ttactccata
      721 actattttgg agttcggcat cctttaaatc tggaaatact gtagatgcta cacataattc
      781 agctacataa ctattgtagt caatttcact tgtatattgt ccagtatgct taccattgtt
      841 accaatcact tttactcttt tagtacactt tcttcttagt gcttcgtctt cttcagatga
      901 taaaactttt aattcccatt caactggttt cccttcttta tctaaaaatc tgttactcgc
      961 tacatatttt acattatcaa cctttattgc attttgactt aaaaaagcac ttaaattact
     1021 catattattc taatctcctt ttattttaat ttttcatata aaaaatacac atatataatt
     1081 tataaatgtg tattttactc cattcctgcc aataaattaa atttttctac taattcccaa
     1141 tcctcaaaag tgaaatccat atcttcatct aaatactcac catcagcatc aaatttagta
     1201 attattccac tgtccatatt acaatcttta agtactactg tctgtcttcc tacagcagat
     1261 gtagggtctt catttgtaac ttgtatgtca aaataaatat cctcaccagt ttctttatat
     1321 ctgtaaagta attctctaaa aatagaagta ttataatgaa atgttgcact tccagtattt
     1381 gtactcccag ttgttttatt tccctttgtt gttcttccta gaattggaac ttcactttta
     1441 tttttttcca ttttagcctc taaatctata gcttgcataa aattatatct tttaccttct
     1501 atagttataa aacattcagc tttctttgca ctaactgtat ctttagcatt tattgtttga
     1561 gccatattat cactctcctc tctaattaac tgaaacagtc atataaagct tactcatagc
     1621 atttattacc ttaacagcat cagatactat gacagttttc ttatcatttc caagctctac
     1681 actaacatca tcagttttaa aatcttctat tgcccttata ttctctaatt ctttatggtg
     1741 tttaacaaca tcattccaga aacttattct tcctgcctta tcattcggaa ctttacctaa
     1801 atacttttca ttaaataaag ttgcaatatc attagcaatt tggtcaagta ctctaacact
     1861 ttggttactt gaaaaatcgt catttttatc atctgtaaat gatacaaaag tatttatatc
     1921 ctctaacaca tgaacttcat ctcccacctt gtggaatata aatttaccag tttttaaagc
     1981 ttcttcaagt tgtatttgtg tataattaac atcaacatca aactcaccat catacttttt
     2041 attagtatta gatttattta tatcgcatcc tgctataact ccagccgccc aataaattaa
     2101 actagattct actaatccaa tatctttaat cttattttct acagacacta caccttcata
     2161 atctgcatca cttttcttat atagtactgt ttgaaactta gctcctacct tatctctcat
     2221 tctctttgta aattctacaa ataaactttt aatttctgtt gttgtagcca aacatcctaa
     2281 agcattaaag ctatagcttt ctattttatc caagaaagct tggtactctg ctcctgtcac
     2341 agcttcgcca ttagttccac cagtaaatac aagtcctgca cttgcttcta gtgttgcatc
     2401 cttcttccaa gtgatatagt cattgtcttg taagtctgta ataacctttg ctatttgagt
     2461 atctaccttc ttattatcta aaagtgttac aacatcaaac ttagcattat catctatatt
     2521 tgttgtaact gttactttta agtcatttcc tctgatacca ctatattttg ctgtggctat
     2581 agtacaactg gctttaacgc ctttatttaa tttataaaaa tatcccaacc ttatattttt
     2641 gaataaatct ctcaaacctt tcagcttctc atgagtataa tcatatccaa aatacttcac
     2701 tgaatacttc tcaaaatcat cactggttac ttgaaatacg tcttcatcta tgccccaatc
     2761 taactctaaa ggtattgcaa caatacctct atccgataat gaactggttg ccctcttagc
     2821 tgagataaaa tttatatagc tacctggtaa tattttattc tgtgttacaa atgttcctcc
     2881 acctaacgcc aaattaactc actcctttca taaagctatt tattatttcc tctacctctg
     2941 agaaggaata taactcattt tcttttaaaa ttgcatttaa taagtctttt ctatttatat
     3001 acttcttaga attaactatc tgctccttag taaacttgta atcggttcct ttacttaatg
     3061 tcttactcaa aattatcacc tctcttcaaa ccaccgaata actctactgt atccatctta
     3121 ttgatatcat tattttttat agtaaagtag ttataatcaa caaagaaatg aagtacatta
     3181 tctacaattt caaagttcat atttgtacct ctgactaaat ctccatcaat ttctatatac
     3241 tctaattcct ccagtagcat ctcagctacc tcatctattt caaatgattt atcattactt
     3301 tttggaaaat aatgtacatc aaaagagttc ttttttaatg tcctgccact tggataggat
     3361 actttgcttg gatttaaagg aacaataaaa aaacaaggtt catttatacc ttgctccaca
     3421 tcttcactat aaattgtata actctctcca aatgttttat ctaatttaat agatattcca
     3481 tcaattatat tattaagcat caaatactcc tttaagcaat attaatagtt ttttctctat
     3541 aatcttatca acttggcttt gtagttccat ctctgaaatt gttaagaaat gttgtccttt
     3601 aacccaacct tttccatctt tagttctatg gccatattca acatatgcat tttgttatcc
     3661 taaaggcttt ttatcctcta gctcttatag tttcctataa gttcggcgta catcatcaac
     3721 aaaataaact ttatttagtt gcccagcact cttggagaga ttatatttat tcactctcta
     3781 cgctctacgg aaacctatag cctattcgca atctataggt tttcctcggt attggcatat
     3841 ataattaatt aaatattttt ccatgttctt ctatggacta tatttgatat agatgaatat
     3901 gttacaggat atatttcact tagttgtttt attgtatatc catcagaata cttttttcta
     3961 atttctttta catcctcaat agataactta gctctgcttt cttcaataat cttaactttt
     4021 tctataagat tatttcctac taatccctta gaatatctac ttcttaagca agaatatgaa
     4081 atacctgttt tttcagaaag ctctatcaat gtaatttctt tttcttcatg ctttactaat
     4141 atatttgttg acctatttct acattgtgtc tttatatcca cccatctaca attgcttgat
     4201 tcataatttc cttcattatt tattctatca attgtaagtt tctctgaata tccattactc
     4261 aatgcccaat ttgcaaaatt atcatagcaa aaccattcat cacacacaat tatattcctt
     4321 tcaccatatc tattgtatct tttattattt ttatctgtac atctagatat tatacccttc
     4381 catatactgt aaagtctttt attttgaacc ttatatttct ttttaaattg gtatttgtct
     4441 gttaaattta atttatcttg ttcttttttt aaacatccac aagattgtac taagccacta
     4501 gttaaagagt cagttcttat ttctttgaaa tttccacaat cacacttaca taaccaatat
     4561 tttctatttc ttttaccact ttttatctct ttagaaaatt taatgactct tagccttcca
     4621 aatttttttc ctgtaatatc taaaaatttt gccataaaat cacctctttt atacattata
     4681 aatcaagagt tattctatgt ccatattcta cctactttat aatgtaacac tattacactt
     4741 agcgtttacc gattttgctg ggttttatat gccccattgt gttaaggcat attcagtcgg
     4801 attaacaacc tctataatat aattatttcc ttgtttatac acaggaagcg acctagcata
     4861 agccactcca ttccatcctt gtcgtaagaa tcctgtatca actggtgttc ttctaattac
     4921 tttcccaagt aatcgtgctg ctaattctct tgctgcatcc ttgcaaaact tatctaaatc
     4981 aatctttgta agctcctcca tctttttaca aactctttta aactctctaa aatcaacact
     5041 gccccatcta gccattatgc tttatcctta aataactcaa gtattatttc ttgatgattt
     5101 ggatatatag ctgattctcc acttcttaca tactctttat catttataat aagttttgaa
     5161 cctgctttaa tttctatatc tggagatata aagagtttaa tagtttgctc tagcttagct
     5221 actttccctt ctgtagcaga aactatattt ttatatgaaa gtttacatgg ttgattttct
     5281 aatacaatca cttctttatt gttagttcgt tttgttacag ggtctttaat tggctgatac
     5341 tcaactatag tacatttatc tctatataac atttctattg cttttctagt tttacttacc
     5401 atcttaagca cctaaaggtt aatatcttat tcttgccata agtagtaaga taagctatta
     5461 agctatcaaa gcgttgttct ggtgtttgag aaccacttcc tatagcaaaa tctacctttg
     5521 tatcaccttc tgatatagac ttttctacag cttcaaagtt aatgctttct atatctaatt
     5581 gacccatatt tttcttggta aataagaact ctccaactat catatcaact tcaatttctt
     5641 tcaattcaat tggcatagtt tttatattac aatctagttt aataatattt tctatttttt
     5701 ctcttacaaa acctattaac cacttatctc catcttttaa tatatatcca aaactttcaa
     5761 gtcttttttc tatttcatca attatattat tttccataat tttcacctac tttttagtaa
     5821 gtttattttt ctctttaagc tgcttatttt cttcttctaa agactcaact tttgacctta
     5881 aaatattatt ttcagctatt aaatctttta cacttaatga cttgccatac tttactgcct
     5941 taccagtttc atctatcaaa tcatatccca tctctaagaa atcatctatt ttacattctt
     6001 ctatggttag tattctattt agttttttta cttgtggcac tatatatcac tcctttttct
     6061 atgcttcaac aacaaattgt attgcatcag cttttttatt taatataaat acatcctcaa
     6121 aactttcttc aaagtagaag
//
LOCUS       DAMJWU010000158         6083 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMJWU010000158 DAMJWU010000000
VERSION     DAMJWU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.163"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            128..1327
                     /locus_tag="PC460_003471"
     CDS             128..1327
                     /locus_tag="PC460_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602320.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEDSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILSSKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1336..2148
                     /locus_tag="PC460_003472"
     CDS             1336..2148
                     /locus_tag="PC460_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602321.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTELWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2159..3427
                     /locus_tag="PC460_003473"
     CDS             2159..3427
                     /locus_tag="PC460_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602322.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            3793..4974
                     /locus_tag="PC460_003474"
     CDS             3793..4974
                     /locus_tag="PC460_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2602323.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(5116..6033)
                     /locus_tag="PC460_003475"
     CDS             complement(5116..6033)
                     /locus_tag="PC460_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602324.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
BASE COUNT         2476 a          588 c          949 g         2070 t
ORIGIN      
        1 atcttatagg atttcaattt aacaatttag tagcagggtt attaagtttt ttaattataa
       61 tgtttattct aaatggaata tcttatttgc taattaaaaa gaaagtatct ctttaaggag
      121 gggtggtatg aaattaaaat ctaaaatatc tatacttatt atatttttga ctttagcaat
      181 atattttagt ataggtttat taaataatga agataattta aagataaaaa ctataaaagg
      241 tgactctaat gaattagacc atataaacat aaattttgga tatgctttga atggatatat
      301 aaattcatat tatacaataa atggaagcaa caaaactaaa aagactgaat taataaaact
      361 acaaaaggat agtaagaaca caaataattt aattgatgta tctagtggtt cagatttaat
      421 tgctcatgtt ggatttgaaa tgaatgagga gtcaaaagat ataaactttg tgatagatga
      481 gaaaaaagaa gatttagaaa aaggtgtatt tccatcagaa gatagtgata aagaaattga
      541 atatgaaaaa ttagatatga ataattttac aaggacaaga tataaagtag ataaaagtac
      601 agatgattat tatgcaaaga tactatcatc aaaaagatat aaaaaatact tatatgtatc
      661 tgtactttat gctacaagtg attcatcaga aaataacaaa gaatattcaa gtactataga
      721 tataataaaa attaatccag aaaataaaaa aatcaagact ataaaaagta taaatttaag
      781 taattatttt tccgaaaaat accaaaacat agagaacata ggtgtaagtt atgctagcaa
      841 atatgaggat aagatgtata ttttagtaaa tgtactaaat acaaacatag gtgataatag
      901 taattctaaa tttcatttac ttgagtataa tcttgaagaa aataaggaaa gcataatgga
      961 attaaaaact gatagcaaaa tggctttagc aaaagcaaag ttagaagcta ataaattata
     1021 tatggtaatg tcgaatgatt atgataatag ttcaattaca gagttaaatt tacttaccta
     1081 taatatatta gataaaagct tagatgaaga taaatatgtt ttagataata gagaaaaaag
     1141 aggaattaga agtattgaaa ttgattcaaa taaaatcgtt gcatatacat caaggctaaa
     1201 ctctatatta tcttgggatg ataaatttat gctatatata ataaataaaa attctaagga
     1261 aattgtatat gaaggagaat tacaatttaa cagttcaaat agtatgttag aatttgaaat
     1321 aaagtaggtg agttgatgaa agatttaata agattattcg aatttgaatt aaaaagaaat
     1381 ataaagaatt atatatttat aataatttgt tgttgttctt ttgtaatttt aaacatagta
     1441 aaaaatttaa atgactataa ttacataata gaaaatgcag ttaaaagtga acagttaaca
     1501 aaaatcggaa atacgataga aacggtaaat gttacaggtt tctcaagctt tagaaatata
     1561 atgggttcta cagaattgtg gtttatgttt ggtataattg catgtatttg ctatgctttt
     1621 tttatttggt atagagactt taatggtaga agtaaatcta tatatacact tataatgcta
     1681 cctaaaaata gaatcaatat ttatatatct aaattattaa atatattatt tctagtgtat
     1741 agctatacag tagtacttac aatatctttg tttattgcaa gtaagttgtt acctagacat
     1801 atgcttggta atgtcacaaa ctatggtttt gtacaagaaa caatatatga gttaaaaatg
     1861 ttacttccat atagctttga aatactattt gtagagtata tatttttatt gataggattt
     1921 gtatctgtag tatttacatc aattcttata aataaatcta tttataaagt aagcacttta
     1981 ataagttttc tatttttagt tatagaaatt ttagtgttta taatttctat agaatttatt
     2041 ataccttacg aatacagtga tgtatttact gttctatatt caagtataaa catatttgta
     2101 tgtagtctta tatctaataa acttttgaag caaaagattg acttttagga gggatattat
     2161 gaaagcaaaa aatataggaa aatatacaat atgttttata atagtatgtt ttttaatgat
     2221 tactatgacc ttaatattga gttattttaa taaggataaa ctatctgtaa aaactttaaa
     2281 aggcaatgta aaagatatag ggagagtaag tgtagtatat acacctaata taagcaaatt
     2341 ttcttatgag gaggtaatta taagtaaaaa tggagtaaga aataatacaa acccaaaatc
     2401 aagaattaga aatgattatt taaatattga caaaaaatac agtgactttg ttaatgataa
     2461 aggaaaagaa tttatatata aagatgattt gaatatagga gaagcgtata cctctgttaa
     2521 gtctgattta gtacaaggaa tgaatgcttt aaatgattgt ataaaggttt caaataaaaa
     2581 tttaaaaagt ggggtagaag aaaattttga tattaattta aataagtctg attttaaaga
     2641 aaatagttgg aaaaccttat ttaacaaaat atataaaggt aatttatata tggtaattag
     2701 tgaaactaat atttcaggtt ctaaagtcaa taatatttgt atattaaaga tagatataaa
     2761 aaataagtct tataaattat taaatagatt taatttagga ttacagaatg gagaacaaca
     2821 aaatatcaat gaaattaaat tttctattgg aagtaaatta tacttagaaa tagtaaataa
     2881 taaaagtaaa aatggatata ttgaaggaca tgatagttac tctaacttta ttatatatga
     2941 tatagaagat aacactttta atataagtaa taaatttatg gataatctaa acaaagaaaa
     3001 gaatgcaaat ccaaaggaga ttggcttatt aagtaaaact aagctagatt atatggtaaa
     3061 aaacaataaa ttgaatctta tagttacgga tatgagtaat tccataacta atcttgttta
     3121 tgatattaat tctaatgaga ttaaattaga agattatgag atacttgatt taaaagtaga
     3181 ttataatgaa gcatcatctg gagttaggaa agttaagcta ctggatgata agatatattc
     3241 tatttcaggt attaagaaca gtatatcagg agaattaaaa ggatttaaag ggattgttga
     3301 tgtagtcgga actaagccag taaaatttca agtatttgat atgaaactta aaaagaatat
     3361 ttatgaagct gagctaataa atggagatat aagtttacaa gaaaagttat tttttgttgc
     3421 aaattaagtg ttagaattaa ttttagaact ttaaactttt atatgtatca cagataagta
     3481 taagtaaggg agtgtctcaa aataaacttt ttagctcatg agacactttt tttatatgaa
     3541 atcaaatata ttttcattgt ttagatttat tttgataatg aaaaaaagat tatctttatt
     3601 ttaagaaaga catttattta taattatata caaaaatatt ttttaatgtt tttgtgtata
     3661 attataacaa tttaatttat attaatgcac ttaaatttga aatagaatgc ttatagtggt
     3721 aaaattaatt attagaatat tagaaataat aaataaataa tacattgtac ttaaaagggg
     3781 gagaaattta agatgaacaa aaaatatatt aaagcaggga atttaattga tggaaccgga
     3841 aatagtatta tgcaaaataa gggtataatt attgatggag atactatagt tgaaattgaa
     3901 gatataagag atgatttaaa tggatatgat gtgtacgatt acttagataa aactgttatg
     3961 ccaggcatta tgaattgtca tgttcacttg acaatggagc cagttggaaa tccaaagata
     4021 tattatgata atgtatcaga tgtagagctt gtagtaaata cgataaaaca acttgatgct
     4081 tatttggatt caggagtaac atatattaga agcttaggtt gtcccaaata tgtggatgta
     4141 caacttaaaa atcttataga caaagggtct atagatggtc ctggtatagt tacttcaggt
     4201 ccagttatat gtatgacagg agggcatgga tattattttg gtattgaaag tgatggagtg
     4261 gatgaatgta gaaaatcagc cagaacagtt ttaaaaaatg gagtagattg tgtaaagata
     4321 atggcaactg gaggtgtaac aactgatgga gttgagccag ggtcaccaca attaactttg
     4381 gaggaaatga aggctgctgt agatgaagct ataaaagcag gtaaaataac tgccacacat
     4441 gcacaaggaa gaactggtat aaaaaatgca gtgcttgcag gaattacttc tatagaacat
     4501 ggtgtttatt tagatgatga aataattgat ttgatgcttg aaagaggaac ttacttagtt
     4561 ccaactgtgg ctgcaccata ttttattcta aatgcaggaa aagaaagtgg aataactgaa
     4621 gctactctta gaaagtgtga gattgttgcc attccacata aagagtcatt cctaaaagca
     4681 tataaaaaag gaattaaaat tgcagttggt tctgatgcag gaacttctta taatgaacat
     4741 ggaaaaacat attatgaaat gaaactaatg tcagattatg gtatggataa tatggatata
     4801 atagttgctg ctacaaaaac agcatctgaa cttttaagaa ttgataaaaa ttatggaact
     4861 ctagaaaaag gaaagaaagc agatgttata gttgtaaatg gaaatccagt agagaatata
     4921 gacgtacttg cagatgtgtt agcagtattt aaacttggta aaaaagttag ataaatagta
     4981 taaaaataaa aaagagtgtc taaagaaatt tttaaataat tatagacact cttttttatt
     5041 agtaataatt aaatctttta tactttagaa aatagattct attttatatt tagatataac
     5101 tctttaaaat cattcttatg caatataatc tatagaatct acacatacaa agtatattct
     5161 tgaatcatta gaatttccaa gtactataac atcatttttc ttacctagaa cttcaacacc
     5221 agttagaacc aaactaccag ctactacagt aacttgatta tttatatttg acatataaag
     5281 attatctagg atacctttat tacaacagca atcatcacaa tcgcagttgc aattacattc
     5341 tttgtaatgt tttttatcaa gatagtaacc taaagatgct atgaaatctt cataatctgc
     5401 aggtgtaata tcaaatgcaa cagctttaag attacacaat gatgctaaat ctacgtttgc
     5461 taatggagta gatgcagcaa gtataaaatc aattatagca gcaagtattg catctaatat
     5521 agccccaagg tctatcgtag gaggaataac tgcatcaata agtgcaatta attcagctac
     5581 tcctggtata gttcctatag tattaattaa tccctcaaga gttaaaggga ctggaatagg
     5641 ataagatact ctaccagcta tatctattaa atcacagtta caagcagaaa atctttcaaa
     5701 agtaccatca agtccagata aattatcttt tggaggagct gaaagatcta tacctaccaa
     5761 attagcacct actataaaga aatctgagat aaaagcaaat tgattaaagt ttacaaaagg
     5821 tcttagagca tcatatctta gaagttctaa tgcttctctc atactttttt tacaacaaca
     5881 ttgttttatt ttaggttctt tatatttatt tggataatta tagtatctat cagctacttg
     5941 ataattttca tagtatcttt catttgtatt tggataatct tcctcatatt cttgtgtaaa
     6001 tctaaagtcc tctctacatt tattattttc cactaaatac actcctttta tattttaatt
     6061 taaatgtata gataattaag ctt
//
LOCUS       DAMJWU010000159         5953 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMJWU010000159 DAMJWU010000000
VERSION     DAMJWU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.164"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..1134
                     /locus_tag="PC460_003476"
     CDS             <1..1134
                     /locus_tag="PC460_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2602325.1"
                     /translation="NIISIEDIESYIISNIDLKNLKIIIKKKKDICDYLNKISNYKKV
                     KSTIEKLETRGKMPKWYLELINKVKNILENMLYNRNLSDEQIFYLNLFLDIQNLFLGI
                     YELKSESVIFENTLDDNKIIAKMDTYIIIDYINASFEYLGYVLNGSALNSIDYGVPQN
                     RERFVLIGAKKDFLKNKKIETPKPIVGENYVTVRDAIGDLAEYEASKGSMDYCFEKNS
                     NNIEKDFYRKIILDSNKIYNHVCTDTRNIALKRFEYINQGNNFHSLPDELKGTYADPE
                     RTQNTIYKRLVYDKPSDTVVNVRKSMWIHPIKNRAVSAREAARLQSFPDSYKFLGTKD
                     SVYQQIGNAVPPLLGRVVAEKILNLFNDEPNEYLRDVFEALDG"
     gene            complement(2134..2706)
                     /locus_tag="PC460_003477"
     CDS             complement(2134..2706)
                     /locus_tag="PC460_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDF2602326.1"
                     /translation="MEKLVKILINIICIIALTFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNINYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNNNDEFKYSLNYIT"
     gene            2975..>5953
                     /gene="cbpA"
                     /locus_tag="PC460_003478"
     CDS             2975..>5953
                     /gene="cbpA"
                     /locus_tag="PC460_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HDF2602327.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAEGDTNAGNAGGDANAGNAGGDANAGNAGGDANAGNAEED
                     SNAGNAEGDANAGNAEEDANAGNAEEDSNAGNAEEDMNALVVEEATAEDPTSVGDFKI
                     SGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKT
                     ASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLTIDDSVVNRD
                     IEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNT
                     ESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTITAKGAYHGAG
                     IGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTN
                     RGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLE
                     KKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDDQGVLYLWFSDQ
                     FKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQYEEFTIDDTPPYLIIKRY
                     DGLPFEEDVEKLFLDWILKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDTNLG
                     SDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYKAEIIPADTETSLVVEESK
                     AKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSE
                     SHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVD
                     GDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDK
                     NIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNG
                     GNSKPDPDNGGNSKPDSGNGG"
BASE COUNT         2292 a          676 c         1069 g         1916 t
ORIGIN      
        1 aatattatta gtatagaaga tattgaatca tatattatat caaatataga cttaaaaaat
       61 ttgaaaataa ttattaagaa aaaaaaggat atatgtgatt atttaaataa aatatctaat
      121 tataagaaag taaaatctac catagaaaaa ttggaaacaa ggggaaaaat gccaaaatgg
      181 tatttagaat taataaataa agtaaaaaat atattagaaa atatgttata taatagaaat
      241 ttatcagatg aacaaatatt ttatttgaac ttattcttgg atattcaaaa tttattttta
      301 ggaatatatg agttaaaatc tgaatctgta attttcgaaa atacacttga tgataataaa
      361 attatagcta aaatggatac ttacattata atagattata taaatgcatc ctttgagtat
      421 ctaggatatg tattaaatgg aagtgcacta aattcaattg attatggagt acctcaaaat
      481 agagaaagat ttgtattgat aggagcaaaa aaagacttct taaaaaataa aaagatagaa
      541 actcctaagc ctatagttgg agaaaattat gttacagtaa gagatgcaat aggcgattta
      601 gctgaatatg aggcttctaa aggtagtatg gattattgtt ttgaaaaaaa tagtaataat
      661 attgaaaaag atttttatag aaagataata ttggatagca ataaaatata caatcatgta
      721 tgtacagata caagaaatat cgcactaaaa agatttgaat atataaatca aggaaataat
      781 tttcattctt tgccagatga gttaaaagga acttatgctg atccagaaag aactcaaaat
      841 actatttata aaagactagt atatgataag ccatcagata ctgttgtaaa tgtaagaaaa
      901 tctatgtgga tacatcctat taaaaacaga gctgttagcg caagagaggc ggctagattg
      961 cagtcattcc ctgatagtta taagttttta ggtacaaaag attctgtata tcaacagata
     1021 ggaaatgcag taccaccttt attaggtcga gtagtagcag aaaagatatt aaatttattt
     1081 aatgatgaac caaatgagta tcttagagat gtgtttgaag cactagatgg atagttcaaa
     1141 taaaatagtg ggtattattc ttgtgattct tatccaacat taatccacga aataaggact
     1201 gtcgaatgag taattttaaa ttgctgtgcg acagttcttt ttttacttgc ttttcattaa
     1261 tttatattta tgcaaaattt gagcttgaaa acggtcataa gaaataaagg tataaaaata
     1321 caattttaaa atagaataat ttacaaagtg taagattaaa aaatattttt gagaggatga
     1381 atatacatac tacatttatt aatgattatc ttatttgtga aacttagaaa tactataggc
     1441 taacactaca ggtataaact ctgaaagaat attttgattg cctttgatta gaattctttt
     1501 aaagttcata ctatgtttta tttgggtgaa agctccttat gcctgaatac ttctattcat
     1561 tctaagttca catccttgtg taccagtgat tctttctaaa ttatcttaat gccaaaatct
     1621 tgttcgcatt tcaaaataaa gtcaggtagc tacttcaaca ttttagttat atttcttcaa
     1681 tagtttgtct ccaaacaaag gtacatctat tagaaacaga tttaatttta tttatatcaa
     1741 taaataatat atactttgaa acttcattaa tatcaccaag ataatttata agttaagcta
     1801 ttatatacta tcttcaccaa ctacatcctc catagtttta cactttctta attcagcttg
     1861 gtaatctaaa tctgtaataa ttatttctct ttgatttttc atttctttgc ctacaaaagt
     1921 ttaatataaa tattattcca tacttaccaa ttttacgttc aaaaaagact gtagataagt
     1981 ctgcatttac acaaacctac ttataggtct cttttttgaa attaatttag catgaactat
     2041 agtaagagca tctttaaatt catagttaca agtatacaaa gttataattt tatctgaaat
     2101 atctaccttt atatcagatt tgtgtactga tttttaggtt atataattca aagaatattt
     2161 aaactcatca ttattattaa agtgtatact taaataatct ctgttaatta agtcagcagt
     2221 atatactgaa aaaatttcat aagtataagt ttgtacttta taatttattt ctactaagtt
     2281 attttctttg aaaaatactt catcagtata gttttctagt tgaccaaaca tagttttatt
     2341 tttcatataa tgaccataga ctattaaact cttatcattt tcaaaattat ttctataatc
     2401 catgaaaata ctacctaaag gaagataatt tttataaaaa tcatgagtta ggtagaaatc
     2461 attgttatat ccttgtacta caggatagtt tatatttacg ttgtttattt taagccagaa
     2521 tctataatca ttatttttat cataaagttc ttgatgtttg ctattattga tagtgttttc
     2581 tctgagttcc gtatatactt catcagcttt tttatattca gataatttta tgtaaatcga
     2641 aaggctagag aaagttagcg caataatgca aataatattt attaatattt ttactagttt
     2701 ttcaattaat taccccttag tattattata taaaaaatta aataaatttt atataattaa
     2761 aatttttatt gtagttattt agcaaataat gaatacttat aatatataag gttgaaaaaa
     2821 acagtatatt cattgacaaa tataagtttt aagtatatat taaaaattat tagagtgcat
     2881 ataaggatgt atacagtgta tattaagaat tattagagtg tatataagga tgtatacagt
     2941 gtatattaaa aattattaag gggaggggga atttatgcaa caaaaatgta aaaagaaagc
     3001 actatcaatg attttaagta tctcaatggg acttggatca gtagcatcat ttaatgcgtc
     3061 aaatactttc gcagatacta tagaagaaag tactaatgca ggaaatgcag aaggagatac
     3121 taatgcagga aatgcagaag gagatactaa tgcaggaaat gcagaaggag atactaatgc
     3181 aggaaatgca ggaggggatg ctaatgcagg aaatgcagga ggggatgcta atgcaggaaa
     3241 tgcaggaggg gatgctaatg caggaaatgc agaagaagat tctaatgcag gaaatgcaga
     3301 aggagatgct aatgcaggaa atgcagaaga agatgctaat gcaggaaatg cagaagaaga
     3361 ttctaatgca ggaaatgcag aagaagatat gaatgcatta gttgtggaag aagccactgc
     3421 agaagatcct acatctgtag gagactttaa aattagtggt ggagaaataa atgtcgattt
     3481 taaatttgca agtggcgttt taactattct tagtggaaaa caaatgacaa ttcaaaataa
     3541 taatccaaat actccaacaa cacataggat tgttatacct aaagatgtaa aagctgaatt
     3601 aatatttgat ggagtaaata ttaaaactgc atctaatagc ccatttactt taacaccaga
     3661 taaagatggt ggtgcatatg cacatgttat attacaagat ggtagtgaaa acaagctaga
     3721 gtcaggtgat gtatattatc caggtatgag agcaggaaaa ggtactactc taactattga
     3781 tgattctgtt gtaaatagag atattgaagg agagtttata gttcctgagg gtggaaaaat
     3841 tcctagagat gtaactctag aaaatggaga caagttaaaa aaaggagatc gccttacact
     3901 actagatagt aataatagag gaagtttaaa agttgttgga aattattatt ctgcaggtat
     3961 tggcggtgga aatactgaaa gtggaggaaa tattattatt aatggtggtg ctataactgc
     4021 tataggtcaa gaatcgagtc gtaatacagg atatggagct ggaataggtg gaggcggtaa
     4081 tggtagtggt ggagatatca caatcaatgg tggtactata actgctaaag gtgcatacca
     4141 tggagctgga ataggtggag gatactatac aacaacaaag aatgcattaa gtccctcagt
     4201 agtagatcct agcacaggtt ctggttatag tggaaatatt acaattaatg gtggactttc
     4261 ctattcatat ggaggtgcac atggtagtgc atttggggat ggatgtgtta atccaggaac
     4321 aaatagagga tatcgtatac ttgtaactgg aggaacgatt ttaccttata tgacaggagt
     4381 gggagattgg atttttgacc taagtgctac aacaggagat gttatagtaa caggtggttc
     4441 attaaaagca acaacattca aaagtttagg tggagcagtt gcatacggtg atttagaaaa
     4501 gaaaacaaaa gtttttatga ataaaatatc tttaaagtat ttaggacaag ataaagttag
     4561 tactacatta gttgatgatt ttaaaatgct aattgatgga aaaatttatc cttatggagc
     4621 acctagttat acagatgatc aaggggttct ttacttatgg ttttctgatc aatttaaggg
     4681 atctgaagta tctgttaatt tagttgttat agataaaatt actggaggaa atataaaacc
     4741 agatgatttt tatgttaaag atataggttc aggttctcaa tttttaaagc aatatgaaga
     4801 atttactatt gatgatactc caccatattt gattattaaa agatatgatg gattaccatt
     4861 tgaagaagat gtagaaaaat tatttttaga ttggatatta aaagatgggg gtattccaat
     4921 aattactcct aaaggtgaaa aactcacaga taaaaaattt atgactatta gttctcaacg
     4981 tttaaatgag gaaacaatgg aaccagatac aaatttagga agtgatgacc ttacaaagca
     5041 agttgatgct ggaaaatatc aattaagcat tacatcaaca cagttttcaa atgtatatcc
     5101 ttttaatgaa gcttattggg ggcatagagc atattataag gcagaaatta ttcctgctga
     5161 tacagaaact tcattagttg tagaagagtc taaagctaaa actgatgttt ttaaaccaac
     5221 agatacgttt acattaaatg ttactatttc accagataag aaagaaggac cagattgtgc
     5281 ctctccaaga ggttacgtac aattctatat taatggaaag gaatataaag atccagtaga
     5341 attaaaaact cataataagg gtagtgagag tcataattat agtactgcat ctattgaatg
     5401 gagaccttta gatagcaatc atcatatttt tactactgaa caaactatta ctgtaaaata
     5461 tattggagat gatataaatt acattgaagg tagtgaggat gaaaagaagt taaagttaga
     5521 tatgacaaat gtagatgtag atggggatgg aataccggat ataaatatag acactgatgg
     5581 tgatggaagg ccagatatca atatagatac gagtggtgat tggaagccag atattaacat
     5641 agatactgat aatacaggtg aatggaagcc tagtacagaa ggtggaaatg gagatggtat
     5701 ttggaaacct gataaaaata tagataccaa tggagatgga aaccctgata cagattataa
     5761 tcgtccagca atagatacag ataatgatgg agtagatgat tattggaagc ctgataaaaa
     5821 tgtagatact ggctcaggag gttatgatac aggtaatcca aatctaaata agccagatcc
     5881 agataatgga ggaaatagca agccggatcc agataatgga ggaaatagca aaccagattc
     5941 aggtaatgga gga
//
LOCUS       DAMJWU010000160         5940 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMJWU010000160 DAMJWU010000000
VERSION     DAMJWU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.165"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            95..1000
                     /locus_tag="PC460_003479"
     CDS             95..1000
                     /locus_tag="PC460_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2602328.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKSVIALWVVLIASVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQALNTDTIRKDIPVSSAVNGVQIWSVTQADENQFSVIFTVEQLITENGSTKT
                     VRSAYEVTVYVDGSGNMVLTKNPTITSIPVKSGYTPKAKESDGTVDAVTTDEINEFLT
                     TFFKLYPTATAKELSYYVNEGVLKPIGKDYVFSELVNPIYNRIDSQVTVSVAVKYLDQ
                     QTKATQVSQFDLVLEKSGSNWKIIK"
     gene            1089..1385
                     /locus_tag="PC460_003480"
     CDS             1089..1385
                     /locus_tag="PC460_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HDF2602329.1"
                     /translation="MELDKIPQWILALEQEDATFLKNFALKSGSLKEIAKMYGVSYPT
                     VRLRLDKLIQKIEMCDQQEEEPFQTFIKGLAVDSRIDVETAKIIIEKYKKEKEK"
     gene            1388..1675
                     /locus_tag="PC460_003481"
     CDS             1388..1675
                     /locus_tag="PC460_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602330.1"
                     /translation="MSTLIIGFIVAGVLLIAEYLLCTKFRSPLWGGIIPLLILAGTIY
                     VFASGRIPFERPYIFPFVVVNTLFFGDWGTGRDKYKKIKQTEMEKMKAKDI"
     gene            1714..1899
                     /locus_tag="PC460_003482"
     CDS             1714..1899
                     /locus_tag="PC460_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDF2602331.1"
                     /translation="MKDKWILCPVCGNKTRLKIRADTELKNFPLFCPKCKTETLINIK
                     HGKVTIIKEPDAKTQSR"
     gene            complement(2018..2377)
                     /locus_tag="PC460_003483"
     CDS             complement(2018..2377)
                     /locus_tag="PC460_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602332.1"
                     /translation="MRKTKETHTFDFRPLGLAIREAREKAGLSRNDLGDKVFYGECHI
                     ADIENIGSHPSFQLFHDLVTMFNISVDEYFYPAEKVAKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRENKK"
     gene            2515..2874
                     /locus_tag="PC460_003484"
     CDS             2515..2874
                     /locus_tag="PC460_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602333.1"
                     /translation="MKLRESYTCPLELVHDIIKGKWKTIIIFQLRNGKCSFSELHHSI
                     AGITQKMLLEQLRELKHFGLIDKKTYDGYPLKVEYFLTGQGEKMLSAIKIMQEIGIDY
                     MVEHGMTEILDEKGICY"
     gene            2964..3515
                     /locus_tag="PC460_003485"
     CDS             2964..3515
                     /locus_tag="PC460_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2602334.1"
                     /translation="MKKALLIIDVQNDYFPNGKCQLYKPEIALNTIKDLLKIFRKQNL
                     PIIYIRHESIHGTFFNPNTNGVQIHNDIKPLDTETVIVKHYPNSFYETNLQNKLVEIE
                     VTKLVVCGMMTHMCIDTTIRAAKDYGYEITLISNGCATKELKWNGVNIPADTVQSIFM
                     ASLNQKFANVITSTEFLSNEITR"
     gene            3499..3864
                     /locus_tag="PC460_003486"
     CDS             3499..3864
                     /locus_tag="PC460_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602335.1"
                     /translation="MKLRDEYTCPLELIHDMIKGKWKPIILWRLRLGATSLSKLENDI
                     DGITQKMLLEHLKELIDYGFVDKKTFSGYPLHVEYFLTEDMGNQILEALRIMQHIGIE
                     YLRKNGMENELTEKHVIPD"
     gene            3952..4452
                     /locus_tag="PC460_003487"
     CDS             3952..4452
                     /locus_tag="PC460_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2602336.1"
                     /translation="MYISSIGISNGIIEKKYGSHGVQFNENDVPTYSIPFKIENAPDK
                     TVSFAFILEDKDAYPVTGFIWVHWLGANLTRSELKENESQTAKDFIQGANSWTSILGN
                     QQTREQTSCYGGMAPPDKAHIYELHVFALDKLLDLKNGFYLNELYQEMEGHILEEAVL
                     KGLYEK"
     gene            4442..4957
                     /locus_tag="PC460_003488"
     CDS             4442..4957
                     /locus_tag="PC460_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2602337.1"
                     /translation="MKSKPVKVGLRTIKTVLSAFLCFLIDSLRASSVPFYAVIASILC
                     IQPDMENSLSVAKNRELSTIIGGILGMLFLLMERIFLPIQPEILRELTLSILLIPIIN
                     LSVLLKQKKGTFLTCVVFLCVTVTHEKDISPIWFAWNRIVDTSLGIGISLAVNSFPFH
                     KFKVGGTMGKY"
     gene            4951..5232
                     /locus_tag="PC460_003489"
     CDS             4951..5232
                     /locus_tag="PC460_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2602338.1"
                     /translation="MLIVEFTEKEDAVFQQAVEWLSSKLAYTPASFNQQKKGFDIDPL
                     TRTFTNEEGTSIYLTAKEFDLLYFLSSHKGQVFTKEQIYEKVWGYDNFY"
     gene            5600..5824
                     /locus_tag="PC460_003490"
     CDS             5600..5824
                     /locus_tag="PC460_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602339.1"
                     /translation="MIGIKKRDIKKALKAGAKAGGSSYADVLADIEATIDEVMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTRV"
     gene            5846..>5940
                     /locus_tag="PC460_003491"
     CDS             5846..>5940
                     /locus_tag="PC460_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2602340.1"
                     /translation="MKNEMWVLCPICNNKTRTKVREDTELINFPL"
BASE COUNT         2105 a          877 c         1201 g         1757 t
ORIGIN      
        1 aatccgatag gatatgcaga cttaaataat agttactggc aacaacactt aatcggtgca
       61 ggacgagtaa aataaaagaa aggacttaga aaatatgttt aagaagaata agaaacagac
      121 agagaatgtc aaagaaaaga aagtgcgtac tgtgaaagtc ggtacacata aaaaatctgt
      181 gattgcattg tgggtagtgc ttatcgcaag cgtgagtttt ggggtatata agaattttac
      241 cgctattgat atgcacaccg tacatgaaac agaaaccata cagttaagac ttaccgatac
      301 caacgggatt gaaaatttcg tgaagaactt tgcgaagtcc tattacacat ggaataacag
      361 caaagaagct attgaagcaa ggacgcaggc aatcagcggt tatctgacaa aggaattgca
      421 ggcacttaat accgatacca tccgcaagga tattccagtg tcttcggcgg tcaatggggt
      481 acagatatgg tctgtcacgc aggcggatga aaaccagttc tccgtgatct tcactgtgga
      541 acagttaatc acggagaatg gaagcacgaa gacggtacgc tccgcctatg aagtcacggt
      601 ctatgtggat ggctctggaa acatggttct cacgaagaat ccgaccatta ccagcatacc
      661 cgtgaaatcc ggctacacgc caaaggcaaa ggaaagcgac ggaacggtgg acgctgtgac
      721 gacggacgaa atcaacgaat ttctgacgac gttctttaag ctctacccaa ccgcaaccgc
      781 taaagagctg tcctattatg taaatgaggg cgtgttaaag cctatcggga aagattatgt
      841 attctcggaa ctggtaaatc ccatctacaa ccgcatcgac agtcaagtga cggtttccgt
      901 ggctgtgaag tacctcgacc agcagacaaa ggcaacgcag gtatcacaat ttgatttggt
      961 gctggaaaaa agcgggagca actggaagat tataaaataa ttattttgat gtttaaattc
     1021 ttattgactt taattttatt tatgatatac tgaatattgt taaagataat atgaaaggag
     1081 ttgtgttttt ggagcttgat aaaatccctc aatggattct tgccttagag caagaggacg
     1141 ctacttttct taaaaacttc gcacttaaat ccggttcatt aaaggaaata gcgaaaatgt
     1201 atggggtatc ttaccctact gttcgattac gtttagacaa attaattcaa aaaattgaaa
     1261 tgtgcgacca gcaggaagaa gaacctttcc aaacttttat taaagggtta gcggttgatt
     1321 ctcgaattga cgtggaaact gcaaagataa ttattgaaaa atataaaaaa gaaaaggaga
     1381 aataaccatg agtacattaa ttattggctt tattgttgca ggagttttat taattgcaga
     1441 gtatcttctc tgcacaaaat ttagaagtcc tctttgggga ggtattattc ccttgcttat
     1501 cttggcagga acaatatatg tttttgcaag tggcagaata ccatttgaaa gaccatatat
     1561 tttcccgttt gttgtagtca atactttatt ttttggagat tggggtactg gcagggataa
     1621 atataaaaag ataaagcaaa ctgaaatgga aaagatgaaa gctaaagata tataaattac
     1681 aaaactgtat acttagatag gaggaacgca ttattgaaag ataaatggat tttatgtcca
     1741 gtttgcggta ataaaacgag gttgaaaatt agagcggaca cagagcttaa aaatttccca
     1801 ctattttgtc cgaagtgcaa aacggaaact ttaatcaata taaagcatgg aaaagtaact
     1861 attatcaaag agccagacgc taagacgcag agccgataat ttgtaggtta ttgtaactta
     1921 tagattgtcg gctcttattt gtttcctgca aagcagatca acaaataagt cttgaagaaa
     1981 tatggctaaa aagaaaacgg ggaaaacaag aagctgatta cttcttattc tccctgctgt
     2041 tcaagatacc gtcaatcgta ccttgaataa tttttaattc gttatcactc aataaatcaa
     2101 gtgatgtttc tatctgacgg cgagctgtgc tttttgcaac tttctctgct ggatagaaat
     2161 attcatcaac cgatatattg aacatggtaa ctaaatcatg gaataattga aaacttgggt
     2221 gtgaaccgat attttcaata tctgcgatat ggcattcccc atagaatacc ttatcgccta
     2281 aatcattacg agaaagccca gctttttcac gggcttctct gattgccagc cctagcggtc
     2341 tgaaatcaaa agtatgtgtt tcttttgttt ttctcatttt aaatcaccat acatgagttt
     2401 atacttcatg gttcgtattt agaatgtgtt aaaagtgcat atattagggt aataaaatat
     2461 gttttgaaat attgagctta aacatgtaag atatttcaca taggagatga taaaatgaaa
     2521 cttagagaaa gctatacatg tccattagaa cttgttcatg atattataaa gggaaaatgg
     2581 aaaacgataa ttatttttca acttagaaat ggaaaatgct ctttttccga actccatcac
     2641 agtatagcag gaattactca aaaaatgcta ttggaacaac tgcgtgaatt aaaacacttt
     2701 ggcttaattg ataaaaaaac atatgatgga tatcctctaa aagtagaata ctttttaact
     2761 ggacagggag aaaagatgct atcagcaatt aagataatgc aagaaattgg tatagattac
     2821 atggtagagc atggtatgac agaaatcctt gatgaaaaag gaatttgtta ttaaactcta
     2881 tcatatacac gaaaaagtaa gtaatttact tagaaactat tatcttatat tatttaatta
     2941 gaattaagag aggatatgag agtatgaaaa aagcattact tattattgat gtgcagaatg
     3001 attattttcc taatggaaag tgccagttgt ataaaccaga gattgcgttg aatacaataa
     3061 aagatttatt gaaaattttt agaaagcaaa acttgccaat tatttacatt cgccatgagt
     3121 ctatccatgg aacatttttt aatccaaata caaatggtgt acaaatccat aatgatatta
     3181 aaccattaga tacagaaaca gttattgtta aacattatcc aaatagtttt tatgaaacga
     3241 atttgcaaaa caaactggta gagattgagg taacgaaatt ggttgtttgt ggaatgatga
     3301 cacatatgtg tattgacaca actataagag ctgccaaaga ttatggctat gagattacac
     3361 taatatcaaa tggttgtgcg accaaggaat taaaatggaa tggagtcaat atcccagcag
     3421 atacagttca aagcatattt atggcttctt taaatcagaa atttgctaat gttattacaa
     3481 gcacggagtt tttgtcaaat gaaattacga gatgaatata catgtccttt ggaattaatt
     3541 catgatatga taaagggaaa atggaagcca attattttat ggcgcttacg attaggtgct
     3601 acttcattat ctaaattaga gaatgatatt gatggaatta cacaaaaaat gcttttggaa
     3661 catttaaagg aacttataga ttatggattt gttgataaaa aaacattttc tggttatcca
     3721 cttcatgtcg agtatttttt gacggaagat atgggaaatc aaatactaga agcattacga
     3781 attatgcaac atatagggat tgaatattta aggaaaaatg gaatggaaaa tgaattaact
     3841 gagaaacacg tgattccaga ctaataccta caaaaaagtg ggtaatttac gttttgcttc
     3901 ctctttgtta atatatatct gtaattaaac gatatacaag gaggaaaatt tatgtatatt
     3961 tcaagtatag gaatttctaa tggaattatt gaaaaaaaat atggtagtca tggagtgcaa
     4021 ttcaatgaaa atgatgtgcc aacgtattca attccgttca aaattgaaaa tgcaccagat
     4081 aaaacggttt cctttgcctt tatactggaa gataaagacg catatccagt gacgggtttt
     4141 atatgggtgc attggttagg agctaatcta acaagaagtg aattaaagga aaacgaaagc
     4201 cagacagcaa aggactttat acaaggtgcg aatagctgga caagcatatt ggggaatcag
     4261 caaaccagag aacagacaag ctgttatggt ggaatggcac caccagataa agcacatatt
     4321 tatgagcttc atgtatttgc ccttgataaa ctgcttgatt taaaaaacgg attttatctt
     4381 aatgaactct atcaagaaat ggaggggcac atccttgaag aagctgtact aaaagggttg
     4441 tatgaaaagt aagcctgtta aagttggatt gcggacaatt aagacggtac tttctgcgtt
     4501 cctttgtttt ttgatagata gcttaagagc ttcaagtgtt cctttttatg cagtgattgc
     4561 gtctatactg tgcatacagc cagatatgga aaacagtttg agcgttgcta aaaacaggga
     4621 actttcaaca attatcggcg gtatactggg aatgctcttt ttgcttatgg aaagaatttt
     4681 tttgcccatt cagccagaaa tacttcggga attgacttta tctattttat tgattccaat
     4741 catcaatctg tctgtactgt tgaaacagaa aaaaggaaca tttttaacgt gcgttgtatt
     4801 cttatgcgta acagtaacac atgagaaaga tataagtcct atttggttcg catggaatag
     4861 gattgttgac acttcattag ggattggtat ttcactggca gtaaactctt tcccctttca
     4921 taaatttaag gtaggtggta ctatgggaaa atactgattg ttgaatttac tgaaaaagag
     4981 gacgctgttt tccaacaagc ggtggaatgg ctgtcctcta aacttgctta cacccctgct
     5041 tcttttaatc agcagaaaaa aggatttgat atagacccat taacccgtac ctttaccaac
     5101 gaagagggaa cgtctatata tctgactgct aaagaatttg acctactata tttcctttcc
     5161 agccacaaag gacaggtgtt cacaaaagaa cagatttatg aaaaggtatg gggatatgac
     5221 aatttttatt agtctggcaa taatgaagaa aaaagaattt tagaaaagtg aggtgtgctt
     5281 atggatttat ctattttggt agtagtattg atattgggtg tgggtattcc tttaagaaat
     5341 aaggcaatca aaaaatacag ataaaatatg aaaaagaaag gaaaaatgaa aaaatatgaa
     5401 tatttagaaa ttgattatac tgcgaaaggt gttgtattcc tttgcaccga taaacataag
     5461 gaagttataa atagctatgc agaaaatggg tatcgctatg cgggttcgtg cctacagaaa
     5521 ttgacgcaaa aggctgtatg cgaaagatag atttaatttt tgaaaaagaa gattaacgta
     5581 ttttagggag ttggttcttg tgataggaat taaaaaacga gatattaaaa aagcattgaa
     5641 agctggtgca aaggctggtg gttcttcgta tgctgatgtt ttggctgata ttgaagcaac
     5701 gattgatgaa gttatgaaca gcaccgaccc agaagtgcag gcaaatttca aaaagtattt
     5761 tggaaataaa cgccctacac cagaggaata tatctataaa atcacgaaga aaacacgagt
     5821 ttaaagaatt agtaagggtg gttctgtgaa aaatgaaatg tgggtattat gtcctatttg
     5881 caataataag acacgaacta aagtgcgaga agatacggaa ttgataaatt ttcctttgtt
//
LOCUS       DAMJWU010000161         5921 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMJWU010000161 DAMJWU010000000
VERSION     DAMJWU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.166"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            180..2204
                     /locus_tag="PC460_003492"
     CDS             180..2204
                     /locus_tag="PC460_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2602341.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            complement(2434..3621)
                     /locus_tag="PC460_003493"
     CDS             complement(2434..3621)
                     /locus_tag="PC460_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF2602342.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3823..5094)
                     /gene="brnQ"
                     /locus_tag="PC460_003494"
     CDS             complement(3823..5094)
                     /gene="brnQ"
                     /locus_tag="PC460_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2602343.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            complement(5235..5888)
                     /locus_tag="PC460_003495"
     CDS             complement(5235..5888)
                     /locus_tag="PC460_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2602344.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
BASE COUNT         2206 a          933 c          707 g         2075 t
ORIGIN      
        1 aagctatttt ccttatcttg caaaaataat tttaaaatag gtttttatag gataaaatcc
       61 taaaaaaacc tattatttaa tcaaatatat aaatttatac tactatttgt tgtataatat
      121 aacttgcctt atactttaaa tactttaaaa ataaatacta ttttgttagg agaaataaaa
      181 tggataaaaa gaagttaatc cttataacac atgataaatt gaatagtgac cactacaaag
      241 aagaattaac aaactttttt ggagacgaaa taatcataga aacacaaaat atacttgatg
      301 gaataaaaga aaatttagag ggagatgtag tgctttctct atcaccttta actagtaatt
      361 ttttaataaa acattttaag gaagatattg aaattattca tggaacgaaa gcactttcaa
      421 aacttggata tgaaaaaatg atgaaactac ctccaggtac aaaatcgtta cttatgacaa
      481 ccaataaaac ttcagcattt gagatggcta cttatctata taaaattggt ataaatcata
      541 tagattttgt accgacatat ccagattgtg atgaaatata tgatttagat acagctataa
      601 caccaggtca gattagattt ataccaaaat acataaaaaa tattgttgat ttaggttgga
      661 gaaaaatctc attagataca tatatgtcat tattagtagt attaaaacta aaaaatgaga
      721 aatttataga aaaactatac aaattgtcca aagagacact aagtcatgat ttcttaaata
      781 catcattaga taatatatct aaattaaaaa ctatacttta catgaccatt gatgaaatag
      841 gtgatggtct aatatttttc aacaccttta acaaggttac ttttgtaaat aagtctttac
      901 ttaatatgtt gggactagat gaaaaactta taaaatcacc ttccttaatg gaatatatgc
      961 caaatagttt cttagataaa attacaaaga acttaaatat cgataatatg attatataca
     1021 tagacgaaat agataaaaaa ttcatacttt ctaaaaaacc attttatctt tataaaaata
     1081 tagagggttg cttaattaca ttgaaagatg taaacaatat agaaatatta gaacaaaaaa
     1141 taagaagtga ttctgtcaaa agaggttatg tagctaaata taaatttaat aatattattg
     1201 gaaatagttc tattataaaa gattgtatta aaaaggcaaa aaaaatggct cttacagata
     1261 atcctatatt aataacaggt gaaacaggaa caggtaaaga agcctttact cagtcaatac
     1321 acaatcattc aaatagaaaa aataaacctt ttgtagcaat aaattgtgct tcattaccaa
     1381 gtgaattact tgaaagtgaa ctatttggat atgaagatgg ttcttttact ggagcaaaaa
     1441 aaggtggaaa aaaaggatta ttcgaattag ctcacactgg cactatattt ttagatgaga
     1501 taggagatat gccacatgat ttacaggtta aacttttaag agtcttacaa gaaaaagaaa
     1561 ttagaaaaat tggtggtaca agtattattc caattgatgt gagaatatta gctgccacaa
     1621 ataaagattt agagaaatta attatagaaa ataaatttag gatggattta ttttatagga
     1681 taagtatgtt cactttagat ttacctcctc ttagaaagag attagaagat atacctcttc
     1741 ttttagaatc atttttaaaa gaacttccct ataaaaacat aaaactcgat aagagtcttt
     1801 tagaagcttt aaattcatac acttggatgg ggaatattag agaactgaga aattgtattg
     1861 aatatatggc ttacatgggt tccaattatc ttacaataaa tgatttacct caaaacatat
     1921 cttcaaaatt aaataataat catatgtcaa gtaatatgtc catattcaat gatttaaacc
     1981 aatatgacaa aaacatatgc atttctatcc taaaatcatt gcacatgaaa ccaatgggga
     2041 ggacaaagtt aatgaaattt atggaatata atgtcacaga atacgaagta agaaatatgc
     2101 tagaatattt gacacgcaat ggatacttaa tttctagtaa aggaagaaaa ggctcatcaa
     2161 taactgagaa aggcataaaa ataatagaaa ataatataat ttaaacctat agtaattcat
     2221 ctttacttta tcaaaattta tatataatta taatttttta tttttctata aattttttat
     2281 aaaataataa aacttattta tattaaaaac gaagctatct caatataggg ataagcttct
     2341 ttttaattgt taaaaagata aaaatatatt tgatttaata gaaaaagagt gcctcataaa
     2401 ctaaaaagtt cattttgaga cacttcctta atattaataa attaattggc ttaaaattac
     2461 tgattgataa ttataaagta cctctgttaa aggagtaaat tctgaaccga ttccaaatct
     2521 taattctgta aacgcctcta aataatctgg gtcacataca tcttcttgtg ttaaatcttg
     2581 tttcaccatt tcagtagcaa gataaattgc tactgatggt agaaaagcat ccattggcat
     2641 aggatatcca ccaagtctat ttgacatgcc aaaagcctta gccttttcac ttatcatgtc
     2701 gtttatttta tcataatttt gagcatcttt ttcagatatt tttagaccca taacactagg
     2761 atatgtttgt attggtgatg gattgctttg ctctgcaact atatacttta attctaaagc
     2821 cttagtcaat ataacttcat ccatatactc atttacacca aatacattta tatcttttcc
     2881 atatttttta acttgttttt caatatcttc atttaaaaac tgcttaactt tgtttttgtc
     2941 ttcttcagta tttatatttg gagtattaac ttgtacaaat ggcaatccta tatttttgca
     3001 agtctcttta atcatttcta atcttttggc tatattaaca tctttcaaat catctgtaga
     3061 ggcataatga ataaaagctt ttgctcccat ttcttttgaa cgttcagcta atacttttcc
     3121 tctttcctct gcactaaccc ctagattcac atcaacaaat tggcttaatt ggtttttatc
     3181 atctcccata ggggcagaaa tagttattat ttctggacgc ttttccttta ccttttgtaa
     3241 agcaggtaaa agccctgctt ggtcagttga aactacaata gcctgaactt ctttatcgtc
     3301 agccaacttt actatcttat taatagcact gtctatatta gaagtaaaat tctctggcaa
     3361 tacaacatgc tttatagttt gtttaacttt agtttgacca tctttgtctt tatcaatttt
     3421 attctcttcc tcatattctt ttgccatctc ttccaccata ttgtattgta ctttattttc
     3481 agataatggt tgagttacca ctgcaacttt aaaatcatct ataattttct ctttatcttt
     3541 cttgcctaca agtaaactac aacctgtaag tccaataatt aaacatatgg atagcataaa
     3601 tgctgttatt tttctaagca ttaattaaaa cctcctaaat ttgtacattc ttatcactat
     3661 tactaatttt atcatacttc aaagaaaaat tatattataa aaaattatat acatgctatt
     3721 ataatccaat ctaaaaagta ctatgggtag attaggttta gattaaacct acccatagta
     3781 ctaaaaatta atatattata aaattaaatt aattacaaat tattatgcta cttgtttgtt
     3841 gctttttata aagttaccaa ctattccacc caatatagct ggtattaccc agttaaagcc
     3901 caaacctgca agtggcaagc tttgtataaa tggaacagct cctgtcattc catttatagc
     3961 tgataataaa ctaatcacta atgtaacata tgctgcacct ttaaaagcat tatcattctt
     4021 tatatgttta ccagccaact ccataacaac tattgctaat aaaacaggat atatagtttg
     4081 taggattgga actgatatgg ctattataga atcaacacct aaatttgata taagcatact
     4141 agctacacat atacctataa ccagttgttt ataactcgcc ttacttattc cttcaaaata
     4201 agttgatatt gatgctgtta aacctgtaga agtagtcaga caggcaagtg ccacaattac
     4261 acctaaaatt acctttccta cttgaccata taacaatata ctagttattt caactaataa
     4321 agatgcttga ggtatactag tatcatataa agttgaaact gttgcaccta aataagtaag
     4381 accaccatat actactgcta gaaatacaca ggctacaaaa ccagctttaa tagtcattcc
     4441 tatttggtct ttatctgatg tatatccttt agctattata gacatcaaca ttatagttgc
     4501 catagatgct gcacctagag cgtccattgt ttgataacct tgtgatatac cttcaccaaa
     4561 tagattttta tctatcatat gctcaggact cataggtcct gctggagaaa ttataccctt
     4621 tactattaaa acaaataata ctaataataa cgctggagtt aaatacttac ctattatgtc
     4681 tataacctta gaagacctaa tagtaaatgc taaagttagc ccaaagaaga ttattgaaaa
     4741 tacagcttta gctactggac catggtctcc aaatataggc ataataccca tttcatatgt
     4801 tgttgcgcaa gttcttggta ttacaagaag tgctaaacat gctagagcag ctacactcat
     4861 tactcttgct aaccctttac cagctctcct aagtacatca tccatgcttc ccttacatct
     4921 tgctgttgct ataataacaa gcattgctat accagcatct gcaacaacaa atccaataag
     4981 aggaattaac cagtctttgc cggattccat ccctaaaaat ggaggaaaca taagatttcc
     5041 tgcaccaaag aacatagaaa ataaagcaaa accaactata aaaatatctc tcatctgctt
     5101 actcacacta aattaccccc tttgaaaata ttttgaatat taagataatc ttactttatt
     5161 ttcagaaaaa tgtcaatctg gtaaaaattc acacattgtt aacgttttcc caatttattt
     5221 tatgaatatt tttttcattt tatgataatt cccatcaatt tcgttaaatc aattgcttgt
     5281 gacacatcaa aaattgctcc tctaatatca tctataccaa tctcaattcc ctcaatatta
     5341 ttagtagtaa aatctatgcc agaaagttta gttccattaa agtttgcttt ttttaaattt
     5401 gtatactcaa atactagatt gtcacctttt acttcttgaa atacagaatt ttcaaaattt
     5461 gaatctaaga tagaaactat cttaaattta gagaatgaaa agttagaata cttacctaaa
     5521 acatccttga acaaaacgtt tttaagtata caatcatcaa atctaactcc tatcaattta
     5581 ctatttttaa attcaactct gtatatactt cctccatcaa aaaatgtatt tgataaattg
     5641 cagttttcaa ataccacatc aagtaaatca atctttttta aattacaatt ttcaaaagac
     5701 acatttctaa atatacaaga ttcaaagcta actcttacag aatctaaatc acttataact
     5761 tcattttcta taattttatt aaatattttt tcatcattaa atatatcttc aagtatatct
     5821 tcaagatttt caataatttc taattctcct gtaaatttag gtttttgcac tttaataatt
     5881 ttagtcattt tcccctccac tataaattat attatttttg a
//
LOCUS       DAMJWU010000162         5879 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMJWU010000162 DAMJWU010000000
VERSION     DAMJWU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.167"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            129..1493
                     /locus_tag="PC460_003496"
     CDS             129..1493
                     /locus_tag="PC460_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2602345.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLVAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1632..2798
                     /locus_tag="PC460_003497"
     CDS             1632..2798
                     /locus_tag="PC460_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2602346.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2915..3697
                     /locus_tag="PC460_003498"
     CDS             2915..3697
                     /locus_tag="PC460_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2602347.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3816..4469)
                     /locus_tag="PC460_003499"
     CDS             complement(3816..4469)
                     /locus_tag="PC460_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2602348.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4493..5857)
                     /locus_tag="PC460_003500"
     CDS             complement(4493..5857)
                     /locus_tag="PC460_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2602349.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2204 a          748 c          900 g         2027 t
ORIGIN      
        1 tttttttatt ggaagattta tgttatattg agatgaattt attatgatat aaaaaataat
       61 gttaggataa atttaaatct ttttctaaaa atgtattaat ataattctta aagaaaggat
      121 ttttaataat ggatataaaa gagttagtta aaatgcaaag aaaatattat aatacaggta
      181 aaacaagaga tatatcgttt cgtatagaac agttgaagaa acttaagtta gttgtatcac
      241 aaaatgaaga aaaaatactt ttagcactaa agaaagattt aaataaatcg gattttgaag
      301 gttttatgac agaagtaggc atgttttata gtgaattaaa ctttgctata aagaatataa
      361 gaaaatggag caaaataaaa agagttaaat caagtatggt taattttcca tctatatcca
      421 aagttgttcc acaaccttat ggtgttactc tcataatgtc accttggaat tatccatttc
      481 aattagcact tataccatta gtttggtcat tagtagcagg gaattgtgtt atattaaaac
      541 cttctgaata ttctgtgtca acatctagtg tagttaaaga catagtggaa gatacttttt
      601 caaaagaata tgttgcagtt gttcaaggaa gtcaagaaga aagtgaaaag ttactattgg
      661 aaagatttga ttatatattt tttacaggaa gtactaatgt tggtaaaata atcatgaaat
      721 cagcatcaga gcatttaact cctattactt tagaacttgg aggaaaaagt ccttgtatca
      781 ttttaaaaga tgctaatata gaccttactg ccaaaagact tacttgggga aaactaataa
      841 atgcaggaca aacctgcgtg gctccagatt atgttttagt gcatgaagat agaaaaaatg
      901 agttaataga gaaaataaaa tattatacta ataagtattt tggagataat ccatgtaata
      961 atgagcaatt tcctaaaatc ataaatcaga gacattttaa tcggatatta tctcttattg
     1021 ataaagataa gatagtctat ggtggaaatt ataataagga gacattaaag atagagccaa
     1081 ctatagtaga caatgttaat tgggatgata atataatgaa ggaagaaata tttgggccta
     1141 tatttcccat tttaacatat aaagatttag atgaagtaat acaaaaaatt atacaaatgc
     1201 caaatccatt ggcactttat atttttacaa aaaataagta cctagaaaat aaacttttag
     1261 aaatgattcc agcaggcgga tgttgtatca atgatacagt tactcatata gctacgaatt
     1321 acttaccttt tggtggtatt ggtgaaagtg gtatgggctc atatcatgga aaagctggat
     1381 tcgatacatt tactcactat aagagtgtgt taaaaaaatt aaatttggat gtccctatta
     1441 gatatgctcc atacgataat aaattaatta aggttcttaa aaagataatg taggttatat
     1501 tgtagaataa taaatataaa taaaattgag tatttgtatt ctgaatatat tttattttta
     1561 atttggtata tttgttataa aagtggtgat tttgtgataa aatttaatta tatagaaata
     1621 aggaggaaaa aatgaaaatt aattggaata caaaatacac aacaatagca atttatacat
     1681 ttataatagc tgctagtagt attatatttt atctagtatc atcacaaatt gatgtatttt
     1741 ctaataattt agatgctata tttacaacat tacaaccttt tataatagga tttgctattg
     1801 catatcttct taattttatt ttaaagtttt atgaagacag aatttttata aaaagtgaga
     1861 aattaaaaaa actaaaacaa agttcaaaaa gaggcttggg tcttttatta acatatgcaa
     1921 cagctacatt gatactttac ttatttatgc atttcgtatt gccacaagta attgagagta
     1981 ttgttggact agcaaatgat atacctatgt atgtaaacaa tgcaactaag cttattgata
     2041 aattaatgac tgatttaaat cttgatgagc aatactttaa tttggcagtt gataagtgga
     2101 atgagtttgt tacatatatt ataaagtttg taacagattt aatcccaata ttaggaagta
     2161 tgttaaagaa tgtagcctct agtatatgga atattgtact aggattaatt gtttcagtat
     2221 acttattaat tgataaagaa aaattctatg gattaagcaa aaaaataact tatgcagtat
     2281 ttacagaaaa acaagctgcc agaattttag aattaactca tagaagtaac tatacatttg
     2341 gaagattttt aggtggcaaa atattagatt cattcattat aggtattcta acatttgtaa
     2401 tacttacgtt agttaaaatg ccatatacat tattaatatc agttataatt ggtattacaa
     2461 atattatccc tttctttgga cctttatttg gagctatacc atctacgcta atagtgatgt
     2521 ttgtatctcc tataaaagct ttctggttat tgttgattat attaataata caacagatag
     2581 atggaaatat tataggacct aaaatacttg gagattcaat tggtatatct gcattttgga
     2641 ttttgttctc cttattggtt gcaggaaaat tattaggttt tattggaatg gttataggag
     2701 ttccaatgtt tgcagtaatt tattctataa ttaaggatat tgttgaaagt aaattggata
     2761 aaaagggatt accgacagat acttccgatt atatgtagct aaaaaatatt atgtataatt
     2821 aaaaatatat aaattaagca atatttaaag cactgagaaa gttaaactct tggtgctttt
     2881 tagatataaa attattatag aagaggtgtt ttttgtggca tttatagaat gtaaatttaa
     2941 atctaaatat actggaggag aaactgatat aattgtaatt ttgcctgtag caaatggaaa
     3001 agaacttttt agtggtaaag agatatatga ttttgaaggt aaatttaaaa cattatactt
     3061 atttcatggt ttagaagatg accaaagctc atggattaga tatactaatg tagagcatta
     3121 tgctaaaaat aaaaatatag ccctagtaat accaagagta gaaactagtt tttataccga
     3181 tatggcgtat ggacaccaat attttacttt tgtaagcgag gaattaccta aatttgttag
     3241 agctatattt cctttatcag ataaaaggga agataacttt gtagctggta tgtctatggg
     3301 gggatatgga gctttaaaat tagcgctttc aaaaccaaaa gagttttcgg cagttgcaag
     3361 tttttctggt tcgcctgata tgattcatga gttagaaaat caacaaatag aaaccaatgc
     3421 agacattcta tttcacgatt ttggtagacc agaagacgct agaaatagtg agaatgattt
     3481 actttacata cttaagaacc ttaaagaaag aaatgaagat attcctaaaa tttatcaatt
     3541 ttgtggtgat agagattttt tatataaaaa taatcagatg tttaaaaaat atgcagattc
     3601 tctgggtgta gatgttgaat atgaagagtg tgtagggggg catgaatggc gtctttggaa
     3661 tttatggata aagaaattta tagatataat tggatagtac acttatggta ttaacaggta
     3721 tactataaaa tatctttaaa tatgataaag actgaaatcc taaaatcaaa ttatgatttt
     3781 aaaatttcag tctctaagaa tttaataaaa ttttattaag ttcttacacg agttattatt
     3841 ggagtaaaat tgtcaactat atctatatag tatttactca attcctctaa aatctcttcg
     3901 cataaatccg tactatgacc aagattaata agaaactgtt ttgtaaaatg ctttattaca
     3961 gcttgagaag atatatcgta cttgtgtgaa gtataaactc taattatttc aggtttcata
     4021 gcaagtatac caatacaaga aagtattaat tcatcatgtt ctatagattc tttatcatta
     4081 acatatcttt tcatgaccat ttcggttaca cttctttgat agcttaaaat agcttcttca
     4141 taagctatat ccaaataaaa atttctatat gcttcgtttt ttagaaggaa acaaaggtca
     4201 gcattcatag caagaaagaa catcatcata gtaccttctt catggtagtt atagattaaa
     4261 gactgcattt catttcttat atcttcatat attatagctc ctatttcgct tttacttgag
     4321 aagtaatagc ttattagacc taaatttata ttagctcttt ctgcaatttg cctagttgtt
     4381 gtcttatgat aaccttgctc aaaaaataat tctctagcag cacttataat actagctttt
     4441 gtttctttgc ccttttctga ttttgacata tttactgtgt atacctctct tttcaatctt
     4501 tttttgaaaa tttgctaact ataaatattg taatagaacc acctattata ggaaccaaaa
     4561 ttctgtcaaa tacagtaaga ataccaatca tatttaataa tgccaatata acacttacta
     4621 taacagcata gatagctcct ttttttgtag cacctttcca gcaaagacca attacaaatg
     4681 gtgcaaatat tacacctgta cataatggag ctggccaaaa caataattct aataaactat
     4741 caaatttaag tgctatgatt gcacctatta tagctgtcaa agctgatgca atttgagaat
     4801 actttagagt attttttaaa tcattaaatt ctatattagg atgtaataat ttgttataaa
     4861 tatctcttga tataatagca ctcattgagg tcattaatga atcggcagta gacattatgg
     4921 cagccaatat agcagctaag aataatacag atattaaagg tggcaaagta gtcgatatca
     4981 tttgaggtaa aacctgaaca gatgatatat ttggaaataa actaccacca atagctccaa
     5041 ttattcctat agggaaagca agaaataata gtataaatcc tgcaattaca ctacttttaa
     5101 ctgcattttt agcattatca gcacattgat atcttaaaaa actttcataa gaaaatattg
     5161 agcctaaaac taatggtaaa actgtataca taaaatcact aatcgattta tttcctggaa
     5221 agaatggttc ttttaccatt gattgtaaca taagtaaagg gttgtcgcca actaatttta
     5281 agcttatata agctactatt ggcaaaaaca ctatacaaat agcaagttga ataaatgctg
     5341 atactgcaac tccccacatt cctgatgtat aagtatatat aatgataact ataactccta
     5401 taattacagc tatatcataa ggaataccaa atgcagtaga tacagaacca aaagaaatca
     5461 attgactacc cataatagct agtatagata ttggtgttac aagactttga taagctctta
     5521 aaaatacact atgaccatat ctagtttcaa tataatcagc aggtgttata tcacaagata
     5581 agcttcttaa ttttcctgcg aaaaatgcat ttataaagca agctaaagct gcacctatag
     5641 tagaatacat tccaacccaa agtcctcttt cagctcccca ttgaactcca ccaactaagc
     5701 accctcctcc aaaagatgta gcagcaatgg ttgcagctag tataggcaaa ggcagatttc
     5761 tacctgcgat taggaaatcg gatgctgttt taactttttt tgaaaaataa aaaccaatcc
     5821 atatgtatat tgcacacgta acaattaaaa gtaacatagt cttttctcct ttataaaat
//
LOCUS       DAMJWU010000163         5777 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMJWU010000163 DAMJWU010000000
VERSION     DAMJWU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.168"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            142..1254
                     /locus_tag="PC460_003501"
     CDS             142..1254
                     /locus_tag="PC460_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2602350.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1462..1938)
                     /locus_tag="PC460_003502"
     CDS             complement(1462..1938)
                     /locus_tag="PC460_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602351.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGDHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2137..3762)
                     /locus_tag="PC460_003503"
     CDS             complement(2137..3762)
                     /locus_tag="PC460_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2602352.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(4100..5119)
                     /locus_tag="PC460_003504"
     CDS             complement(4100..5119)
                     /locus_tag="PC460_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF2602353.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(5116..5685)
                     /gene="mocA"
                     /locus_tag="PC460_003505"
     CDS             complement(5116..5685)
                     /gene="mocA"
                     /locus_tag="PC460_003505"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF2602354.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         1974 a          969 c          595 g         2239 t
ORIGIN      
        1 attctaatat ttgacataat atatacattt tttaaataaa tatatacaat gtattaaaat
       61 gtatgcaact ctatttcaaa attattttgt gatatatata tgttataata aatgtataaa
      121 attttgattg gtgggttata tatgaatgca tttattagaa aaagaaataa aaactatgtt
      181 gtttacctgg aatttgaaga tgaaaaaaca ggtaaaagaa aacaaaaaaa tatgggtact
      241 tttgataaaa aaagggaagc aagtaaaaga ctaaatgagg taaaagaaag cttatataat
      301 gatggttttt tagtaccaag cgaaattaca gtgtcagaat ttctattaga ttttctaaat
      361 aaatatagtg aaaatatatc tttagctaca tataataatt atgttagtat ttgtaagaat
      421 tacattaatc catctattgg aaaatataaa atacaagact tacatccggt tcacatacaa
      481 aactacatag ataaactatc ttataaacta aatcctcaat caattaagat tcatataaat
      541 atattgaaac ttgcaataaa aagagcttat agactcaaat taataaaaga aaatgttatg
      601 gatagtatag aggctccaag atataaaaaa tttaaaaatg agatttatga tagagaacaa
      661 atgataaaat tattagattt agcaaaaaat acagaaatgg aattacctat taatttggct
      721 attggattag gtcttagaat ttcagaagtt ttaggtctaa cttgggataa tattgatttt
      781 gaagaaaata caataacagt aaataagata acttcccgaa ttaatggttc tgttattctc
      841 aaagagccaa agacagaaag ctctgttaga aaaatatctt ctcccaaaga attaatgtct
      901 ttattaaaag aatataaaat aaaacagaat aaaaatttat taaaatcttc cattagaaat
      961 aataataatt tgttgttttt caataagaaa tgtgaaccta ttgctgaaga tgttatgagt
     1021 aaaaaattta aaagattttt agaaaagaat gaattacctc atattagatt ccatgattta
     1081 agacactcac atgttacttt acttataaat tctaaagtac ctataaaggt tatatccgag
     1141 agagtaggac actcaaatat aagtactact ctaagcgttt attctcatgt acttaaagaa
     1201 atggataagg aggcttctga caaaatttct gaaagtttat ttaatgctaa ttaatattaa
     1261 tttatcttag ctttaaatta gcttagctta ttgagataga ttcattaata ttaaatgtat
     1321 ctatctcaaa ttaataacta cttaaataat ctgttttatt ttaatttatc gcctcagtta
     1381 ttatactttt ttacgaaaaa ttatacttta caataaaaaa gatattccct tatagaaaat
     1441 atctttttaa atactatatt attatatact tgctaacatt ggaatatcta acatcttctt
     1501 cattacatca tctacttttt ttgctttata ttcattgtat ccaattaaat atcctttttc
     1561 atatgaactt tttaaaatct ctttttcata taaattatca gatttggctt ctgtgacact
     1621 taaattataa agccctttac acccatcttc ataaccttta ttataccctt tttctgcaaa
     1681 atagttttta tgattttttt cgttatgtac tgaagatatt gttactccag aattttcttt
     1741 tgtactagca taaggacaaa aacctttatt gtgttgatgt gctgaatgtc ctccacaata
     1801 ataacgtgtt aactctgatt tatttttatt atgatgatct cctgatgaat tatgccctcc
     1861 tgagtgagca tatgaaaaat tccctacact aatcatagct aaactaatta ctacagctac
     1921 taaccttttc ttaaccataa tctattcccc ccaaaatgtt atatattttt attatatcat
     1981 ctactatatt atatttaaat tcttaatata caaaatatta ctctattctg ctaataaaca
     2041 tgtttaaaca acataaaaaa cctcctaata tttgattaaa aatttacaca ctcatgcata
     2101 gcatgtctca aatattaaaa ggttttatac taataattat ttatcttttc catacatttt
     2161 agctaattgg tcttgtatct ctttactttc taaatactca tcaaagtcca tttttctatc
     2221 cattattcca tttggagtta tctctataat tctatttgct atagtagata taaattcatg
     2281 gtcatgagaa gtaaataaaa gcactcctgg gaacttttca agtcctttat ttacagaagt
     2341 tatactttct aggtctaagt ggttggtagg gtcatctaat acaagtacat ttgcatttga
     2401 aagcattagc ttactaagca tacatctaac cttttctcct ccagatataa cttgagcttc
     2461 ttttaatgct tcttcacctg agaataacat tctacctaaa aatcctctta taaaactttc
     2521 acttttttct tcagagaact gtctaagcca gtcaaccaaa gaatattcta ctccgtcaaa
     2581 aaacttatta tgattttttg gaagataatc ttgagaagta gttactcccc atttatactc
     2641 tccactgtct ggttcaagtt ctcccataac tatgttgaaa agtgcagtag tagctatttc
     2701 atctcccata aatactactt tatcatctct atccaatcta aatgacacat tatcaagaac
     2761 cttaacccca tctatagttt tagttaaatt gtgtacttct aaaacttcat tcccaatttc
     2821 tcttgcaggt gtaaatccta catatggata tcttcttctt gatggttgta tatcttcaac
     2881 ttctaatttc tctaactgtt tctttcttga tgttgcttgt ttagcctttg aagcattaga
     2941 actaaatcta gctataaatt cctttaattg agctatcttt tcttcagtct ttttattttg
     3001 gtccttagcc agttgtaaag ccaattgact tgactcatac cagaaatcat agtttccaac
     3061 gtacatttgt attttaccaa aatctacgtc aactatatta gtacatattt gatttaggaa
     3121 atgtctatca tgtgatacta ttataactat agattcttct aaatccatta taaaattatt
     3181 taaccagttt attgatttaa aatctaagtg gtttgtaggt tcgtccatta atagtatttc
     3241 aggcttacca aaaagtgatt gtgctaacaa taccttaacc ttttccccac caactagttc
     3301 cttcatttgt ttgtaatgca tatctttcgt tatgccaaga cccataagta ttttctcagc
     3361 attagtttct gcatcccatc cgtcaagctc tgcaaactca ccttcaagtt cggcagcttt
     3421 tatcccatct tcttcattaa agtcttcctt catatacaat gcatcttttt ctttcattat
     3481 gttccaaagt ctctcatgac ctcttataac aacatttaaa acagtttctt cttcatattc
     3541 aaagtggtcc tgttttaaaa ctgacattct ttctttatca gttatagata tactacctgt
     3601 attaggctct atttctcctg ataatatttt taaaaaagta gattttcccg caccattagc
     3661 tcctattatt ccatagcaat tacctttagt gaattttaag ttaacatctt taaatagttc
     3721 cttatcacca aatctaagtc caacacctgt aacttgtaac attaatatat tttcctttct
     3781 ttttgctcat aatttatttt atcataaaac tttgataaca tttttattat atattatcac
     3841 gtaaatagta tattttttat actaaatttg tgaaatattt attaaaataa tactaaaaat
     3901 tgtaaattta taaaaattaa caatctctta tattatactt ataaagaaat caaattgcaa
     3961 ctttattcat tcttttacac attgaaaata agttattata atttctaaat aaatatgctt
     4021 ttcttaaaac atcattctag ctgttttgtt tcatatttaa aaagagtact taatttgtaa
     4081 atactctttt ttaataactt tatattttag caaaattaca tgcaggataa gtacatacat
     4141 cacagttcat acaaaggcca ccatgaccat atctagctat atcatactta tcaagctttt
     4201 catcaattaa aaccctagat aaaactatat caaatactgt agttttgtga tacattgcgc
     4261 atcctggtat acccataatt ggaacttctc cataataagc aagtaaaaac atagctcctg
     4321 gtaatattgg tgaaccatat gtcactaaat cagctccagt ttcttttata gctgttggtg
     4381 taacatcatc tgggtctaca ctcatcccac cagtacaaca gataagttct gctccttgac
     4441 ttataaattc ttttattgca ctctttataa tttctttatc atctgggcat atagtctgtc
     4501 ctataacttc acaaccaaag ccttttactt tttcttctat tacgggtcca aatttatcaa
     4561 caattctgct ataaaacact tcatttcctg tagtaacaat tcctactttc ttaggtttaa
     4621 aaggtactac atttactatt tttctattac caactacttt ttttgcatct tctaattttt
     4681 tctcatctat aactaaaggt atcactcttg ttccagctac ttttaaacct ttatttacta
     4741 taaaattatt atgaagagta gccatcatta tttctccaac acaattcaaa tcaaaaagtg
     4801 catcaacatc tatctttaat agaccatcac atgctgctat aaaatctatt ttcccttctt
     4861 ttatttcact aaaatctaaa ttttctccag cagttagttc ttttaaaaac aatgcgcctt
     4921 cattttcatg aaccattcct tctgactttt cccatacata caaattatct ttaccaagag
     4981 ataataacac tggaatatct tcttctttta ctatgtggcc ctttttaaat tttcttcctt
     5041 taaattctcc aggtattatt tgagttatgt cgtgacataa aacttgtcca acagcatcta
     5101 tagtttttat tagcttcata atttaccccc aattctctca taatccattt ttgtatcgat
     5161 atcaaataac atttctttag gaacttctac atactttatt tttgatgaat tttttaaaat
     5221 ggttttacct ttttcatctt ctttaagtgc caacaattct tctttcttac tatatgggaa
     5281 tatgactgga tttccacaca tatctttgta tcttggaatt actataaaat ctctatctaa
     5341 tttaaaagta tttataattt tatctatata taagttatca ataaatggct ggtctcctac
     5401 aaagaacata taaccatcac attttgagct atttaaaact cctaatttta tagattcact
     5461 ttgacctata tttgcatttt tattatctat atattcaaaa ccatatttat tacacaataa
     5521 caaaacctct tcatattgac ttactactac aacttgatga aaatcaatct tgctaacttc
     5581 tctaaataca tgttctatta tagaacagcc tttataattt aataaaagtt tatttgctcc
     5641 cattcttctg gcaaagcctg atgccattat cactgcattt atcatatata tcatccttgg
     5701 tatttataat atattgatag ttctatgatt tttataatat ctctattgat attatctata
     5761 tatataatat catataa
//
LOCUS       DAMJWU010000164         5748 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMJWU010000164 DAMJWU010000000
VERSION     DAMJWU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.169"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(224..2056)
                     /locus_tag="PC460_003506"
     CDS             complement(224..2056)
                     /locus_tag="PC460_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602355.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVE
                     HGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2049..4295)
                     /locus_tag="PC460_003507"
     CDS             complement(2049..4295)
                     /locus_tag="PC460_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602356.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4363..4824)
                     /locus_tag="PC460_003508"
     CDS             complement(4363..4824)
                     /locus_tag="PC460_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2602357.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5129..5695)
                     /locus_tag="PC460_003509"
     CDS             complement(5129..5695)
                     /locus_tag="PC460_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2602358.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1823 a         1075 c          630 g         2220 t
ORIGIN      
        1 cattttcctt actacatatt ataataaaat aaacactttg taaagacata aaattatgtg
       61 gctatctcaa aatatagaga atcctctttt tcattgttaa aatgataaaa atacacttta
      121 tttcatacac aaaagagtgc cttatgaatt aaaaagttca ttttaaggca ccctcattta
      181 ttttatctta ttaattacat gtctatgcta agatagtttt ttgttacatt gcttcatctt
      241 cttcaaattg agaattgtaa agattggcat agaatccgcc tttctttaga agttcctcat
      301 gatttccttg ttcaactata tctccttcat tcattacaag aatcatatca gcatcacgta
      361 tagttgataa tctgtgggct ataacaaaac ttgttctacc ttccataaga ttatccatag
      421 ctttttgaat caatacttct gttctagtat cgacagaact tgtagcttca tctaatatta
      481 aaattttagg atcagctaaa atagcacgag ctatagtcaa taattgtttt tgaccttgag
      541 aaacattact tgcttcttca tttaatttca tcttatatcc atctggtaat gtcttaataa
      601 atctatgaac atgtgcagat ttagcagcct ctataacttc ttcatctgta gcattcaact
      661 taccatatct tatattttcc ataattgaac cactaaataa ccatgtatct tgaagaacca
      721 taccaaacat ttctctaagc tcactacgat taaaatcttt aatattatga ccatcaatca
      781 gtattgaacc gctattaaca tcataaaatc tcattaacag tttaactatt gtagttttac
      841 ctgctcctgt aggtccaaca atagcaactt tttgaccatg ttcaacattt actgagaaat
      901 catttattat agtcttgttt gggttatatc caaagttgac attttcaaaa tcaattttac
      961 cttctaaacc atctattgaa actgcatttt caacaatttg gacttcttct tcctcttcta
     1021 aaaactcaaa tactctttca gaagctgctg ctgttgattg caataaattg gcaacctgtg
     1081 caatctgagt cattggctgt gtaaagtttc ttacatattg tataaatgac tgtatgtcac
     1141 caacctcaat tgttttctta attgcaagcc aacctcctaa gattgatacc attacatatc
     1201 ccaagtttcc aataaacatc ataagtggtt gcattatacc tgataaaaat tgagatttcc
     1261 acgctgaatt atacaggcta tcatttagtt tatcaaactc ttcaattact tcatcctcac
     1321 gattaaatgc ttttactatt gtttggccac tgtaagtttc ctcaacttga ccatttacat
     1381 ttcctaaata ttcttgttga tatctaaagt atttttgtga tttctttatt attcttgaaa
     1441 ttacaaacat tgatattggt aatattaaaa ctgccactaa tgtcataatt ccacttatag
     1501 ataacatcat tatcaaaaca cctatcatag ttgttactga tgttataagc tgtgtcatac
     1561 tttgatttaa actttgattt aaagtatcta tatcattcgt aattctcgat aatatttcac
     1621 catgcgtctt agtatcaaaa tacttcattg gcaatctatc caattttgct gaaatctctt
     1681 ttcttaaatt ataggatact ttttgagaaa ttccactcat tataaatcct tgtataaaag
     1741 agaacagtgc acttatcaaa taaaggaaaa gtaaaaaggt taagattttt ccaattgcat
     1801 taaaatctat acctacgttt cctccagaaa ctttactcac tagcccttca aatatctttg
     1861 tagttgcctt tcctaaaatt tttggaccta ctatagaaaa cgacgcacta ccaatagcaa
     1921 atacaattac tagaattatt gatattttaa atttagataa atatgcaatt agttttcgca
     1981 tagtcccttt aaagtcattt gctttttgtc ctgctcccat agattttccc ataggacctc
     2041 ttcccattct actcattagc tagctcctcc tttgaaagtt gtgataaagc tatttgctca
     2101 taaacttcgc aattttttag aagctcttta tgtgttccta ttccaactat ttcgccctca
     2161 tttaaaacaa ttatctgttc tgcatttaag atagtactaa ttctttgtgc aactattaga
     2221 actgtacttt cagcagtttt tgattttaaa gccttacgaa gagctgcatc agtttttaag
     2281 tcaagtgctg aaaaactatc atcaaatata taaacttctg gattttttgc tattgctctt
     2341 gcaatagaaa gtctttgttt ttgacctcca gatacatttg ttccaccttg agaaatttca
     2401 gttttaaaag cttccggttt agaatctata aattctgtag cttgagctat ttcagctgct
     2461 gttctcattt cttcatctgt tgcgtctttt ctaccatatt ttaagttgga ttctatagta
     2521 ccagaaaaca atactccctt ttgaggtaca tatccaattt tttcccttaa atcatgttga
     2581 gaaacatttt ttatattaac tccaccaagt aatatttctc cttcagtaac atcaaagaaa
     2641 cgaggaatta aatttataag agttgactta ccactaccag tacttcctat aaaggcagtt
     2701 gtttctcctg gctttgctgt aaaagatata tttgttaata taggttcttc tgcgtctgga
     2761 tacatgaacg aaacattttt aaattcaaca agaccctttt tatcttcctt aaactcttgt
     2821 gcctctgatt tttctgggtc ttttatgctt atatctgaca ctaatacttc atctatacgt
     2881 gtagctgaaa cggatgctct tggaagcata atagatacca ttgatatcat taagaatgac
     2941 attattattt gcattgtata ttgtataaat gccatcatat ctccaacttg cattgctcca
     3001 gaatcaacac tgtatgcacc tctccaaact ataagaactg tgataaggtt cattataagc
     3061 atcatagcag gcatcataca tgtcataact ctatttacaa aaagatttgt tttagttaaa
     3121 tttttatttg ctttatcaaa tctttcttct tcatgttttt cagtactaaa agctctgata
     3181 actggaatac cagtaattat ttcacggctt actaagttta acttatcaac aagcttttga
     3241 accagtttaa acttaggcat aaccactgag aataacacta caattaatga taagatagca
     3301 actactgcta ctgctataat ccaggccatt gaagtatttg tgtttaaaac ctttatcact
     3361 ccacctatag ccattatagg tgcatagaat actattctaa gcaacataac catcaacatt
     3421 tgtatctgtt gaatatcatt tgtactacga gtaattaatg aagctgtaga aaattcattc
     3481 atttcagtat ttgaaaatga cataactttt ctaaatactc cacttcttaa atttctacct
     3541 acacttgcgg ctactcttgc tgctaaaaat cctactgtta ttgtagctac catactaatc
     3601 attgctatac caagcatttt agcaccagta aataatatat aattattttg agttttttct
     3661 gtatcaatac ctattttctc atattctgac cttacatatg aaacagagct ttgtgttatc
     3721 atactctctg gcatactttc aaagcctttg ctcatctctt tagtcaatgt atctaactgt
     3781 tcctttggca ttgctgataa caatttaaat atatcaacat ctccactttg agtagcttgt
     3841 ggtggaagtt tactcattaa ctgagctttc atagctttta cctctttact atcgccctca
     3901 agccctgata ctattaaaat agctttacca aaaataacat ttaaatcatc tatagtgtct
     3961 ttgtctttag tatttaactc ataaagagat tcatctttta gacctgggta cttatcttta
     4021 tacttaacat aatccttttt attcagcaaa gtataattat ctagtacttt atccttacta
     4081 cttttatcca taaataaagt aattttattt aattcactct ctctaatcac agatggaaca
     4141 gagttttcta ctccaccttg ttgtatacct acatttacta tattggatgt gtattctggt
     4201 aaagaaaggt cacataccgc ttgaattacc aatagacaga ctattattac tattgataat
     4261 gccgatttct ttaaatattt aattaattta aacatacatt tcttcccttc tgtaatatta
     4321 attagtctaa taacattaaa ttgaaaaaca ttattttaaa ctttaaaaat atttttcatc
     4381 ttcatgctta tgacatcctg cttttaaatt atctcgtact tgcattagaa gtcttctaag
     4441 tactattttt tcttcttcac taaatcctaa aaaaaactct ctttccaatt ccttgttaaa
     4501 ttcttcaact ttattacata tttctaaacc tttgtctgtc aaaaaaatcc tagatactct
     4561 ttggtccttt tcatcttgtt ttctttccac aagtccagct ttttccatcc tctttatcat
     4621 aactgtagtt gtagatgctt ttactttcct tttatctgct aattcttttt gactttgccc
     4681 atctttcatc tttaaagaaa aaagtatttg gggttgacca tgatataagc ctaatttatc
     4741 aaaataaaaa tgagttctta taaaatatat tcgcattaat tgcaagaaaa catcatttaa
     4801 tttttcttca gaattgctat tcatacttgc cctccttatt ttttatatta cttagtcggc
     4861 taagcattat tttactatac tgcatgcatt ttttcaatat atttataaaa aattcatata
     4921 aatttcattt attaaatatt tttatatcat atagttataa ttttaataaa taatagtaag
     4981 atttttaatt agtcagctac ttatattttt tattgtaaag cttaaaataa accttttcat
     5041 atattttcaa ataaaaaagt tgtcccaaaa taaattttga aacaacatct tattcaacag
     5101 tatcttgatt atataacatt atgatactct acttatcttc atatccattt acaacaacat
     5161 ctaacttccc agtttcaggt gatataatta gaccatgtac aataatacca tctggaatta
     5221 aaggatgccc ttttattaaa tcaatactat cttttacaga ttcttctgca gaatcgaatc
     5281 catgtaacca ttgttttgta tcaattccag cactacgaag tgtaagtatt acatcattag
     5341 atattcctct atctaaaatt ttaccaagta acttatccgt atcaacatta cacataccac
     5401 atccatgatg ccctacaacc ataacctcat ctacatcaaa ttcgtaaatt gcaactaaaa
     5461 cacttctcat aatacttcca aaaggatgca ttatagtagc tcctgcattt tttattagtt
     5521 tcacatctcc atttttcaaa ttcatagcct taggcaaaag ctcagttaat cttgtatcca
     5581 tacaagataa gactactatt tttttatttg gatgttttga tgtcacatat tgttcatatt
     5641 ctttgttctc tacaaatgat ttattatatt ctaatatctc ttcaagtttt ctcatatgta
     5701 atatcaccct ctttaaaaat ttttttatta ttatatcact attaaagt
//
LOCUS       DAMJWU010000165         5718 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMJWU010000165 DAMJWU010000000
VERSION     DAMJWU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.170"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            19..276
                     /locus_tag="PC460_003510"
     CDS             19..276
                     /locus_tag="PC460_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602359.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(386..1066)
                     /locus_tag="PC460_003511"
     CDS             complement(386..1066)
                     /locus_tag="PC460_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2602360.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            1326..1625
                     /locus_tag="PC460_003512"
     CDS             1326..1625
                     /locus_tag="PC460_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602361.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            1949..3604
                     /gene="ilvD"
                     /locus_tag="PC460_003513"
     CDS             1949..3604
                     /gene="ilvD"
                     /locus_tag="PC460_003513"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF2602362.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLNFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            3948..4580
                     /locus_tag="PC460_003514"
     CDS             3948..4580
                     /locus_tag="PC460_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2602363.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            4603..5415
                     /locus_tag="PC460_003515"
     CDS             4603..5415
                     /locus_tag="PC460_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2602364.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         2251 a          649 c          947 g         1871 t
ORIGIN      
        1 gtaaatggag gaattgatat gataataatt agaagtcaag ataaaactaa tttaatgcag
       61 ataaaccaaa ttaaaataga tggtagtcaa gtttatgcag tatttgaaag taaaattgat
      121 actgtaaaaa taggagatta tgaaaataat acaagagcta tacaagtgtt ggacaatata
      181 caaaacttca ttgaaaatgg aacaaaatat gattatataa catcaaataa agtcagatac
      241 aatgttaata aaatatttca gatgccagct aaatagtgag tgtgcatttt aatttaataa
      301 ctaaagccca ctttcttttg tggtatgaaa gagggcttta catatttgta aatatcgaag
      361 tacacttgtt ttatcaataa attatttaat aaggtataat ggaccaatta aacaataaaa
      421 attctcctaa aacagcaagt attaaatata taatagcttc atttttgtaa agagaggtaa
      481 ttttttcgct tatgtcatca tcaattgtaa tagcaatttc atcttgaata atatttttat
      541 atatagatgg gaaaatatct tttttcttta taagattaaa taaattattt acacctaccc
      601 atataaaata tcctaaaaca gcacctaatg tgttcatcat taaatcatca atatctgtag
      661 ctcttaagtt aaacaattga caaagttcaa ttgctaatga aaaacctaat ccagttaatg
      721 aaacattgat aatatttcta aaatttttcc aaattaaagg tagtaaaaat cctaaaggca
      781 taaacataat tatatttaaa atatatgtta agatgccaac tgagtcaaaa ggtattagat
      841 ttatttcatc aattctaaaa agcccactat accttcctat atcccatatg ctaccaattc
      901 cagctttact aagtgctaga tagatatata ataaaaaaat atatacccat actaaatgta
      961 tcattacatt cttttcaact ttcaaatttt ttcgttttat aacgactatt tggtatatta
     1021 agcatggtaa tataacgcaa ataaatgaat aaaatgtcca tatcatttta ttttgtcccc
     1081 tttcaaattt atgtttttag ttttataagt actatcttta tattaaactg taagttttaa
     1141 aaatctttta ataaaatctt aaaaatttat taatttataa aaatatatta ttaagattaa
     1201 aaaatatata taatcaaagt ttctacaatt atatagttag ggatataaaa attttatcgt
     1261 atgcataaat attataaata tatggaaata ataatagtat attttggtaa agtaggagga
     1321 attttatgaa atggattaaa tggtatagta ttacatgcat atgtattttt atagtagtag
     1381 ctttttatat gtttatattt cctaataaaa tagaaactat tgatacttca tctgcatata
     1441 gctttgttga aaagaaagta ccaaatagtg ctgtgtatca aggatacaaa aagaatccag
     1501 ttgatggtac tactacaatt tattacagct atgataattc aactcatata gttagattat
     1561 cacatccaga agactattca aggaagatta attgggataa agtttcaaac atcagatttg
     1621 attaatcttt aatatttata aaagtctata tatctaaggc aaatatatta ttaatcttat
     1681 tcatagttga gcactttcta ttatagaagt gcctcttctg atttatatca taaaatatta
     1741 ctcaacaatt tgtaaaatat attagtttat aaaatatatt gaaaagataa tattatataa
     1801 tacaattgtg taagatattg ataaaaaatc gtaataaatt ttcattagta tatgctaata
     1861 tttttttaca attttcgtaa ttaattaata gaaaaatata ttataataat attatgaaaa
     1921 tttaaaaaag taagaaagag gtaatattat gagaagtgat ataaaaaaag gaatagaagg
     1981 agctccaaag agagcgttaa tgtatggaat gggtcttact aaagaagaaa ttgaaagacc
     2041 attaataggt atagtaaatg cacaaaatga ggtgattcct ggtcatctgc atcttgatga
     2101 aatagcagaa gctgcaaaaa atggagtaag aatgagtggt ggtttgccat tagaatttcc
     2161 tgctataggg gtatgtgatg gtatagctat ggggcatgtt ggaatgaatt attccctggc
     2221 ttcaagagaa ttaatagcag attcaataga agctatggct atggcacatg gttttgatgc
     2281 tttggtactt atacctaatt gtgacaagat agtgccaggt atgttaatgg ctgctgcaag
     2341 acttaatata ccaagtattg ttgtgagtgg agggcctatg cttccaggta aaaaaaatgg
     2401 aaaagtatat gattttaact ctgcaatgga gggtgttgga gcatgtaaag atggaacagt
     2461 aagtgaagaa gaattagaag cgttagccat gaactcttgt cctggatgtg gttcatgttc
     2521 tggtcttttc acagcaaata gcatgaattg tttaacagaa gctttaggca tgggtatacc
     2581 atataatggt actgctgcgt cacattctgg agaaagaaaa aggatagcaa aatatgcagg
     2641 tatgtatgtt atggagttac ttaagaacga cataaaacct agagatattt taacaataga
     2701 tgcttttaaa aatgctatag ctgtggatat ggcaatggct ggttctacaa atacagtact
     2761 tcacttacct gcaatagctt atgaatcagg aatagagctt aacttaaatt tttttgatga
     2821 aataagtgaa aaaactcctt gtttaacaaa attaagtcca agtggaaaac atcatattga
     2881 agatttacat atggcaggag gaataccagc tataatgaac gaactttcaa agataaatgg
     2941 aataaattta gattgcaaaa ccgtaacagg caagactata ggggaaaata taagaaattg
     3001 tgaaatagaa aatgaagaag taatacatac attaaagaat ccatatagta accaaggtgg
     3061 gcttgcaata ttgaaaggaa atcttgctct aaatggagct gttgtaaaaa agtcagcagt
     3121 tgcagaagaa atgttagttc atgaaggacc agcaagagtt tttaattcag aagaagaagc
     3181 tgtaaatgct atttttggta aaaaaataaa caaaggtgat gttatagtta taagatatga
     3241 aggtccaaag ggtggtccag gaatgaaaga aatgctatct cctacatcag cagttgcagg
     3301 aatgggactt gataagcatg tagcacttct tactgatggt cgtttttcag gagcaactag
     3361 aggagcatct ataggccata tttctccaga agctatggaa ggtggtttaa tcgggttagt
     3421 tgaagaagga gatataattt ctataaacat accagacaaa aaattagagt taaaagtaga
     3481 tgaagttgaa atagaaaata gaaaattaaa atttaaacct ttagaaccga aaataaagca
     3541 tggatactta agtagatatg ctaaactggt aacatcagca aatacaggag cagttttaaa
     3601 atagtagact atatttatgt aaattagaaa aagttaagaa aatattaatt ggatatctta
     3661 ggtgttatag cactaatgag gtatcctttt tattttgtat gaaaaacaca gcaaaatctt
     3721 gattatcttt gaaaacaata catctctatt ataaggaata cgagtacagc atagagatgt
     3781 atcttttaga ccttgttgag gtaatacaac tttttaaaag tttgatgtgg aatgcttttc
     3841 gttccatctg aactatgaag aaattgaaag tcagttaatt cttgacataa taaatattaa
     3901 aatgtataat aaaaaacaaa cagacagtct gtttttagga gggaaatatg aatagagaag
     3961 aaaaaagtaa aaatagtaaa gaaaaaatta tccaatcagc attttcacta ttttcgtcta
     4021 aaggatatga ttcaacatct acacaagata ttatcaattt atctggtcta tctagaggtg
     4081 caatgtatca tcactttaaa actaaagaag atatactgag aagtgtcaca aaagaacttt
     4141 actcacaaat gaataatttt ttagagcatc ttgttgctga tgacaccctt acagcaaatg
     4201 aaaaaataat agaattggtt gttcatagtg cgaatgatta cacacgtaga aaaatggtac
     4261 attgtagctg gttagaaaaa atcccattcg ctttaataga ggaagttcgt aatctcaaca
     4321 atgtagttgc acccaatatt gctaagataa ttaaacaagg tgttgaaaat aaagaatttt
     4381 cttgtgaata tccagaggaa ttagctgaaa tgctcgtttt tagtattgat attttacttg
     4441 accctgtatt atttaaacgt gaatacagtg aagtatgtaa taggttggat tttttattat
     4501 tcatgttaaa aaaaatggat attcctctaa ttgatgagta tggtattcaa aaatttaaag
     4561 atttatttag acaattataa taggaaaagc gaggaataga ctatgtttta taatgcaaaa
     4621 agttgtcaaa tgatgatagg aagtaataca gtgaattata ttgaatttgg tagtggtaag
     4681 aagacactaa ttattttacc aggattagga gatggtttat ttcctttaca tggaaaaata
     4741 caggcgattg cttttgcttt tagatataag caatttgcaa aagattataa ggtttatgta
     4801 tttagtagaa aaaatcaaat tacagaaaaa tattccacaa gggatatggc taaagaccaa
     4861 gcagatataa tgaaaaaact tggaattatg aaagcagaag taatgggtgt ttctcaaggt
     4921 ggaatgatag ctcaatatct ggcaattgat tatcctgagt tagttgagaa acttgtgttg
     4981 gcagttactt cttcaaagca aaatgatact atccaaaatg ttatttgtag ttggattgat
     5041 atggcgaaga agcagaatta caatgattta atgattgaca cagcaaagaa atcctattct
     5101 gaaagatatt taaaaaaata ccaattattt attccttttt taggaaaagt aggtaaacca
     5161 aaggatttta aacgatttat tattcaagcg acctcttgca tagaacataa tgccttttcc
     5221 gaattaaata aaataacatg tccaacctta attataggag gtgctaatga taaaattgtt
     5281 ggaaataatg cttcttttca tttagcagaa aagattaaaa aaagcgaaat atttatctat
     5341 gagggattgg gacacgctac atatgaagaa gcacaggatt ttaatgaaag agttttagag
     5401 tttttaaata agtaaacacc tttgttaaaa atatacatat gaagattgga aatttaatgt
     5461 taaaaataga aacatgaaaa tatgcttaac tggtattttt actattcata accaattttt
     5521 gatacattat ctactataaa tacaaatata gcttcaatgt gattatatat tagtgtattg
     5581 gtaaagcact tatacaaaca gaggaatttt gtaaattcat attataccca catttgttaa
     5641 cttatgaaaa tataatcaaa atttttatga gatttttata taaaaaatat aatgtttgta
     5701 tacaaaaata ttgaagat
//
LOCUS       DAMJWU010000166         5652 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMJWU010000166 DAMJWU010000000
VERSION     DAMJWU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.171"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            240..1067
                     /locus_tag="PC460_003516"
     CDS             240..1067
                     /locus_tag="PC460_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2602365.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2067..2530
                     /locus_tag="PC460_003517"
                     /pseudo
     CDS             2067..2530
                     /locus_tag="PC460_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(2797..3441)
                     /locus_tag="PC460_003518"
     CDS             complement(2797..3441)
                     /locus_tag="PC460_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602366.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3371..4660)
                     /locus_tag="PC460_003519"
     CDS             complement(3371..4660)
                     /locus_tag="PC460_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602367.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4657..5529)
                     /locus_tag="PC460_003520"
     CDS             complement(4657..5529)
                     /locus_tag="PC460_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602368.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2048 a          781 c          701 g         2122 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatgt
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 gtggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattataaa
     1141 atcagagtgt ggaaatattt atgtgtattc tttaaataaa aatttccaca ttctgtaaag
     1201 ttattatact tgatatataa aattcaattt aggaacatag tctaacattt ataaacatat
     1261 taaggtaatg atttctcttg gtagttattc tcatatctta ttctttgatt atatttatct
     1321 tattcctagc ccttatgtca tattatattt cttatcttat tatttataat tagcattaac
     1381 ttatctaaaa tcttgtattt ttttaatagt aaagtttaca tctatattaa tttcataaaa
     1441 caatcaaggt agtcaagaga ttcaacttta tttgatttta aaaatttaag tgaatttgtt
     1501 cttatacaca cctttttaaa attatatctg tagaaaatat catttagagc tataattaaa
     1561 ttaacttatt aaattttaga aaatttatta aaaaatataa gttaaattaa tatttttaaa
     1621 gattcttttt tgtaactact agaacactaa tttagtagtt attgtatctc taaagtttaa
     1681 gcatggatag atatacattt gataatttgc ctataattta cctactactt ggattattgg
     1741 agcaaataca atatattaat aataagttcc taaaaccaat aaattaacta atacacataa
     1801 agatattgat gttgattata caactataag aaacggattt aacagaattt aatcaagcta
     1861 agaaattata tatatcgtga tgtttttgaa ttagaaggtg attcaaaatt agtttatagt
     1921 cttctgtgaa tccaataaaa agtacagggt acaagaattg ttagtaaaat ttttaaagat
     1981 tttatggata aatataaata gttttactta ttttctaaaa ctttaaaata ccattaatta
     2041 ccttaaatat tttaatagga ggacatatga atttaaataa agttaaattt actgaagaac
     2101 atgctattga agttactaat tggaaatatg aaggtaaata ttcaatatac aatctacctc
     2161 catgggagga aattaaaaag aagaattttt cactagcaaa agaagataaa agaaaaaatt
     2221 ttataagttt tataaatgac aataaagaat tgataggttt tataaactta ttagatgaag
     2281 gaagctctgt attctttggt attggttgaa accgaattat tgtggaatgg gaattggtaa
     2341 agaaataatt agcctaggat tacaagaatg tagaaataaa tatagtacta aaccaattgt
     2401 attaaatgta agaacctgga acatgagagc tgtaaaatgt tatgagtctc aaggatttaa
     2461 aatagttgag actaaggtac aaaaaacgca tttaggagat ggagaatttt ttgttatgag
     2521 gtatcaatag tttataacaa tagtcctatt gttaatacaa caattaaaat gaaaaaatac
     2581 atatgtgtag gtcatagcaa taagtgtctt tgaaatataa atttgagagc ttttgataaa
     2641 attcggtata catatatatt ttatactacg gatatataaa tctatctaca tacataaaga
     2701 gattgtaagt aggtttgtgt atataaaaat agatttatat ttcataaatg tactttttat
     2761 ttgcattacc tttattttat ctaagctctc attttattat ctaatcttcc tttttactgg
     2821 ttttattaag agagatgcaa ttatcaacac aacacttcca agtatcagcc aatatttctc
     2881 atcaaaacta ccttgactca tagacattat aaaaaatttt ccaacatact tgcctttagt
     2941 catcatattt aaaattaaat atcctataga tatcatataa cctatgattg tattattaaa
     3001 taaatttgag aaaaacattc ctaaacttcc tataaacata gctgatgcga aacttccaat
     3061 aatatactca actactggaa aattacatcc aagatatatt aaaacattta caaaacctat
     3121 gattaatacc actataccta taattgacat cagtatccta attaattgta caataacttg
     3181 agataatgct tttgaagata ctatctcatc aatttctttt gattgctcta gcgaaaatac
     3241 aggtaccaac attactatcc ctattattgc aacataattt tctaaacata tagctgttga
     3301 aattctgtct aaattagcta ctccttgtat tagaggaatc atacatagca atataattga
     3361 acatattaaa ttagttaaag tgcttaattt gaagttttct tttattagaa ctaaacagtt
     3421 ttgaaaactt tgcccaaaca tcaagattcc cccttctttt ttgctcatat aagaatatag
     3481 ctaacaaaac aagcaccaat gctcccaaac ttatcacaat tctattaatc ataatttgat
     3541 tgaagttttg tataataata tccccattta atacactatt atgtcttatc attagcccca
     3601 ttgttgaata atcacctttg agtcctgtag aatttatact gtaaagccac caaattaatt
     3661 gcacaataat tgctactggt gtatctgtta atatagtaat actcattcct acagctgtaa
     3721 ctatcataag agttggtagt atccaccata atgtatattt tataaatgct aagttatcaa
     3781 tagatatacc tttatcttta gcaaagaaaa tcaaaggaag taaatctttc atacctaaaa
     3841 ttaatacagg caatatcatc ataaagacaa gtgcaaaata tcttgataaa actattttgc
     3901 atgaagatac ttgttttgaa taaataatct ctctcattct tgaacgctta tctttaagtg
     3961 acataaaaac tgccacaaat actggaaata tacttaaaac tatacctata taatcgcaaa
     4021 aaattcttgc atacccatta ctaactttat ctttttgtat tgtatagttg taattcttca
     4081 tagcttcatc ataggtaact ggaactactc caaatctttg taacttgtca aaagcatatg
     4141 cagaccctcc tcctaaaatg tcgtctactc tttgcattat ttcttcaaat ctttcaagag
     4201 atactttaga ataatctatt tttatacttt catcagtttt ccaaagtgaa tttatttcgc
     4261 taatactttt accagttatt tcttctatat atttttctat ggtcttcgtt ttattttggt
     4321 ctaattttat atacttaact acccctgttg gatacgttgt atatgtgttt ttttgatatt
     4381 ctctaattag gtttatagtt gcatttggaa tgactttttc tgttttcata gattcttttt
     4441 gaccatatgg atattgattg tgtacagagt aatattcctc aatattctct actttttgtg
     4501 gagcaactaa tggatttcca tcattaggaa tatttttact tacatcattt gaaactgaac
     4561 ttccaagttg agaaatatag aaaaaaacta aaactacaat aaaaatataa tatatcatac
     4621 tctttaatat atttttacat tcttttataa atagattcat atattatctc ctcccattat
     4681 aagcatatag ccatcttcaa tagttggatt acaatttact gctgtctcaa aaggtttatc
     4741 atctgatata atcctaagta tagcctttcc accatgagtc aacattccta taactataaa
     4801 tctacttttt atattttcaa tatctttttt atctacttca atactatata ctttcccttc
     4861 agctagtttt gctaatgctt ctgttgagcc attgtatatg acttttccac tattcaatat
     4921 agctacgttt tcacatgtag cttctatatc tccaacaata tgtgtagata aaataacgat
     4981 tctatcattt gcaatttcgc atagtaaatt tctaaatctt atacgttctt ctgggtcaag
     5041 acctgctgtc ggctcatcta ctattaaaac tctgggatta tgaattaatg cttgtgctat
     5101 cccaagcctt ctattcatac ctccagataa agattttact ttcacatttt gataattagt
     5161 aaggtttact ttgcttaata actttgctat aatttttctt ctttttctta gtggtatatc
     5221 cgataaaact gctaaataat ccattgcttt atatactgtc atgttaggat acattgaaaa
     5281 gtcttgtggc aaataaccaa ccatatttct aatttcctta gtattctcaa taggaactcc
     5341 acacatatgt attgaacctt ctgtttttga taataaagtg gctaaaattc tcataaatgt
     5401 ggtttttcct gctccatttc ttcctaaaag accaaacata ccactgtcga tttcaagatt
     5461 tatattatca agagcttgtt ttttcccaaa tcttttacta agattggata ctgctatttt
     5521 catttccata tataaataca cccccagaaa caattttata gaattttatt tttataaatt
     5581 tttactgtat tttcataata aattctaaaa cttaagatat tatttataaa tttctaaatt
     5641 ttttctaaag at
//
LOCUS       DAMJWU010000167         5545 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMJWU010000167 DAMJWU010000000
VERSION     DAMJWU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.172"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            140..2641
                     /locus_tag="PC460_003521"
     CDS             140..2641
                     /locus_tag="PC460_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF2602369.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQNVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2801..3469
                     /locus_tag="PC460_003522"
     CDS             2801..3469
                     /locus_tag="PC460_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2602370.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3453..4730
                     /locus_tag="PC460_003523"
     CDS             3453..4730
                     /locus_tag="PC460_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2602371.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5131..5352
                     /locus_tag="PC460_003524"
     CDS             5131..5352
                     /locus_tag="PC460_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602372.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         2342 a          558 c          982 g         1663 t
ORIGIN      
        1 aaaaaatatt gatttaatat acctataggg ggtataatga aaatatagat ttctaaataa
       61 gtaatttatc aatattacat atcaaaataa aacgttacaa attagctgaa aatatattga
      121 aagaaaggaa gattttatta tgagttcaaa taaagtggta gaaagttata aaattacagg
      181 tatgacatgt gcagcttgtg caaaggcagt agagagagta actaagaaga tggatggagt
      241 atatgaccaa aatgtaaata tagctactga aaaacttaaa atagaatatg ataattctaa
      301 ggttaatttt gatgatataa agcaggttgt agaaaaagca ggatatggaa ttataaagga
      361 agaaagtaat aaaaaaattg atatgaaaat tgatggaatg acatgtgcag cttgtgcaaa
      421 agctgtagag agagtggtaa aaaaattgga tggagtagaa agtattagtg tgaatattgc
      481 aacagataaa gctaatatag actatgaccc atcaaaagta aaattatctc aaataaaagc
      541 agctattgaa aaagctggat ataaaccaat agaagaggta agaaataagg ttgatgtgga
      601 tgaagataaa ttgagaaaag aaagagaaat gaagtcttta tttgttaaat ttatagttgc
      661 aatagtattt gcagtacctc tgttttatat agcaatggga cctatgataa taaagcctat
      721 agggccatgg cctttgccag aaattataaa tcctatgaca aatactttta attatgcatt
      781 aattcagtta attttagtaa taccagtaat gatagctggt tataaatttt atataaatgg
      841 gtttaaatca cttttttctt taagccctaa tatggattct ttagttgcaa taggaacatt
      901 agccgcattt ttatatagtc tatatacgac actacaaata gccaatggac aaattcaagg
      961 tatgcaccat catcagttgt attatgaaag tgcaggaata ataatagcac ttatcttact
     1021 tggtaaatat ttagaatcaa aatcaaaagg aaaaacatca gaagcaatta aaaagcttat
     1081 ggggctacaa cctaaaactg caatagtgtt agttgatggt aaagaagttg aaactcctat
     1141 agaagaagta gagataggag atatactatt agtaaaacca ggaactaaaa tacccgtgga
     1201 tggagttgta atagaaggat atacatcagt tgatgaatca atgcttacag gtgaaagtat
     1261 accagtagaa aagaatgttg gaagtaaggt gacaggagct agtattaata aaaatggtgt
     1321 tattaagttt aaggctgaaa aaattggtgg agatactgct cttgcccaaa taataaagtt
     1381 agttgaagat gcacaaggta caaaagcacc tattgctaaa cttgccgata ctgtatctgg
     1441 atattttgta ccaatagtaa ttgcaatagc tgtagttgca tcattattgt ggtttttaat
     1501 aggaggaaaa gatatagtat ttgtacttac tatatttata tcagtacttg taattgcttg
     1561 tccttgtgca ttaggattag caacaccaac tgctataatg gttgggactg gaaagggcgc
     1621 agaaaatgga atcttgataa aaggtggaga ggcactagaa tctgcacata aagtaaatac
     1681 agtgatattt gataagacag gtactataac agaaggaaag ccaaaagtaa cagatatagt
     1741 attaaataat aatgtaaaag aagaatatct tatcaagatt gcttcaagtg cagaaaaagg
     1801 ttcagaacat cctcttggag aagcaatagt taagtatgga gaagagaaga atataaaatt
     1861 tgaaaaagtt gataatttta aagctatacc aggtgctggt atacaagtta ctattaatga
     1921 tgaaagtata ttacttggaa atagaaagct tatgaatgac aataatataa agcttggaga
     1981 tttagaagaa aaatcaaata tattggcaag ccaaggtaaa acacctatgt atatagctgt
     2041 agatggaaat ttatcaggta taatagcagt tgcagatgta gtcaaagaaa gtagtaaaaa
     2101 agcaatagaa atacttcatg atatgggaat aaaagttgca atggtaactg gagataatgc
     2161 gaaaactgca aatgctatag caaatcaagt cggtatagat atggttttgg cagaggtttt
     2221 accagaagat aagtcaaaag aagttgaaaa actacaaaat caaggaaagt ttgttgcaat
     2281 ggtaggggat ggaataaatg atgccccagc tcttgcaaaa gcagatatag ggatagcaat
     2341 aggaagtgga acggatgtag ccattgaatc tgcggatata gtacttatga agagtgattt
     2401 aattgatgtt ccaacagcaa ttaaacttag tcatgaaact ataaaaaata taaaacaaaa
     2461 cctattttgg gcatttggat ataatacaat tggtatacct gtagcagcag gaatattata
     2521 tgtatttgga ggacctttat taaacccaat gattgcagca gcagcaatga gcttaagttc
     2581 agtttcagtc gtatcaaatg cacttagact taaaaacttc aaggcatata agagagatta
     2641 gattaataaa ttaaataaaa tgtatccaaa agagctatct ttaaattaaa aggattaatt
     2701 tacaaattaa gatagctctt ttttcatgta aaattcacaa caataagata aaatataagt
     2761 aatatacgaa attttaaata aaatagaaag gattgataga atgaattcgt caatacttgt
     2821 aattgaagat gattcaaata tacaagagtt aatatcagaa tttttaagtg cagaaggtta
     2881 tcaagttgat acagcaaatg atgggttgga aggtatacaa aagtttaagc aaggaagtta
     2941 tgatttagta atattagata taatgatgcc aaacttagat ggttatggag tttgtaaaat
     3001 gataagaaag tcatcaagcg ttccaattat atttttaact gctttaaatg atgaaggaga
     3061 ccaacttaaa gggtttgact tagaatgtga tgattatata acaaaaccat tttcatttaa
     3121 tcttcttata aaaagagtag aagcaatttt aaggcgaagt aacaagacta taaatgacaa
     3181 atttatagtt tttgaaaaat taaaattaga tttaaacaca tatattgcag agatagatgg
     3241 agaacctata gaacttacac taaaagagtt taatatatta aaagctttga tagaaaagta
     3301 tccacaagtt ataactaggg aaggtctttt ggatagtata tggggttatg actattatgg
     3361 agatactagg attgttgatg cacatataaa aaatataaga aaaaaaatat cattaccata
     3421 tattaaaact gtaaaaggaa taggatatac acttgagaag gatatttgat aagtgggaaa
     3481 agttaagtat aaagtataag ttattttcaa taacgacatc attattaata gcattagctt
     3541 taataatata tctaatttta tattttttat tgccatcata ttatcatgag tataaaattg
     3601 agtctcttca agaaagtttg aaatctttag tagatagttc aattcatttc gatacatata
     3661 ctttagaaga aagactttat tatatggcta aagaccaaaa tttggctatt ttattaaaag
     3721 ataaccaagg taaaattgta tatggtaaga atgaagtagt tattttaaga tatagtaagt
     3781 acatgattaa tagtcttgag gatgagtata gaacttctat acctatatat actaaagatg
     3841 caaaagatgg tccatatact ttagaactcg ttatgccatt acaaccaatt gacgaagcaa
     3901 acgaagttat aagaaaacta atgccataca tcatatcaat agcaatattg atagcgatta
     3961 ttggagcata tatatattca attgtaataa caaagcctct tataaacata atagagagtg
     4021 agagagaaca ggaatataga aggaaggatt ttgttgctac aatatcacat gaactaaaaa
     4081 ctccaataac cataataagt ggtcaaattg aaggtatgat ttatagtgta ggtaagtata
     4141 aggataggga tacttatctg aaaaaatcgt atgagtgtac acaagaatta aaagatttag
     4201 taaatgaaat gatagaggtt tcaaaatcag aaatacttga aaaagatttg aaattggtat
     4261 caataaatat aagtgaatta ttaaatagac ttgtaaagag acaagtattt ttaatagaag
     4321 aaaaacatat gaaaactatt ttaaaaatag aagaaaattt ggaaattaaa gcagaccaag
     4381 agagaataac taaggcaatc aataatataa taaataatgc cataaaatat tctcctgaag
     4441 aatcagagct tataattaga ctctatgata aaaataaacg aataagtaaa aagacttcta
     4501 atcaaagagt ggttttagaa attgaaaata ctggtgtaac tatcgagaaa cgttatttag
     4561 aagaaatatt taatccattt tatagaatag aaaaatctcg ttcaagaaaa actggaggaa
     4621 gtggacttgg actttatata gtcagccaaa tttttaaaag tcatgggttt gattatagca
     4681 taaaaaacaa agaaaactct gtagtattca cagtagaatt taaaaattaa ttacttatta
     4741 taaagaatat ataactttca ataatagttc attttaaaat cacaaaaaat tcataatgaa
     4801 actgtattat ataaatataa agaagcttaa aaccataaaa tacaccaact ataatgatga
     4861 gatttcctta catcctccat ataagtcatc attatagtaa cccaacaatt aagttactat
     4921 gaatttaaaa aataacaccg agcatataat ctatgcttag tgttattttt attttgaaat
     4981 aaaaaatatt taatttattg aaatgtatag atttagatag tttaaactat atttaagact
     5041 taaaaattat aattgatatt acaaataaat atatgtactt tgggaaaaat aaatatagga
     5101 agtattatat ttacagagga ggtagttttg atggatagat taaaaagaga tatgaaaaat
     5161 atgaaagaag atttaaaaga aggtgttaaa aagacagctt atgttatgaa aaatacagct
     5221 gaagatttga aagatgatgc acaaggtgct atggtaaact tagaaatgaa aaaagatgag
     5281 atgatggaaa gatatgaaca aaagaaattt tctaaagaat tagagaagca aatgaagaat
     5341 gaaatgaaat aagcttttat ttatgaataa caaattattg tattcatata aaaagtagcc
     5401 agtaatatta aatagtttat ttaaggaaat agactttcta aaaataactg tattagattt
     5461 agaaagtcta tttcaaatta gttttataaa tagacataag gataaagtag atgttataga
     5521 actagatttt aaaaaataga tacta
//
LOCUS       DAMJWU010000168         5538 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMJWU010000168 DAMJWU010000000
VERSION     DAMJWU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.173"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(285..968)
                     /locus_tag="PC460_003525"
     CDS             complement(285..968)
                     /locus_tag="PC460_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF2602373.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAVIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(1144..1734)
                     /gene="zapA"
                     /locus_tag="PC460_003526"
     CDS             complement(1144..1734)
                     /gene="zapA"
                     /locus_tag="PC460_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF2602374.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1759..4152)
                     /locus_tag="PC460_003527"
     CDS             complement(1759..4152)
                     /locus_tag="PC460_003527"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF2602375.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4169..5188)
                     /gene="pheS"
                     /locus_tag="PC460_003528"
     CDS             complement(4169..5188)
                     /gene="pheS"
                     /locus_tag="PC460_003528"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF2602376.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5238..5472)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1682 a         1091 c          604 g         2161 t
ORIGIN      
        1 cttgaaatac ttctattaat ctatatattg cctactatac ttaagacatt ttaattaatt
       61 tatacactgt ttactatact tgaaatactt ctattaatct atatattgcc tactatactt
      121 aagacatttt aattaattta tacactgctt actatactta aaatacttct attaacttat
      181 acattgctta ctatatttta aattctttaa ttaatctatg caatatttgc tatatataaa
      241 gtatatcaaa taacagcttc atttttttgt aaatttacag aaagttaatt ttcattaaat
      301 gaatcatctt tattgataaa cataattcca aagaccactg ccaaaataaa aaaacatatt
      361 agtattgatt tttccacgaa aatttttgta aaaaaagttc ctaaaatagc tccagatata
      421 aagaatataa taacagcata atactgtaaa ctctttttta tcataaaaat attcttagta
      481 tgtatataat gaaataaagt ttctgtagca cttcttaaat ttccagtaca cattgtcgtt
      541 gcaaaactat tcccatttac ttttctaaaa ctctctactt gcattgaaca aataaaagaa
      601 atagcaacat ttacaaacat attaaatctt cctctaggaa taaatgaaac aataagtatt
      661 gtaatcattt caattataat gataacctga cgccaatgaa tctttgtctt attatttgta
      721 aaatactttc ttatcttctc ggctataaat acacctaaca taaatgccaa aataggaatc
      781 aaataataaa atgattcctt tacattacct tctgctatat taagcccaaa caaaactata
      841 tttccagttt gtgcattagc aaagacttta cctcttacca cataagtata aatatccaaa
      901 aatcctcctg ttattgccaa caaagaacat aaaaagaatg attcagacat ctgactcttg
      961 cttgtcatat ataaacctct cgctttctaa tgatagatac aaaatcaaaa tcattttaga
     1021 ttctattttt cattgatata aaaaaattat ttatatttaa atattgtcta tatttataca
     1081 tcatttatat ttatacatta aaagaagctg tctcaaaaaa caagagacag caaatctttt
     1141 agtttattcc acattttttg catcattatt taaccctgta cattttaact gtaaatcata
     1201 agctttgttt tgaaactcag aagctaagtt ttcagctata gtagctcttt catttgcttc
     1261 ttcaagttga gtacttaact cctcaatctt gtttttataa gcatctaact ctgctctaga
     1321 accttctgtc gtattactaa gttcgaatat ttcttgcttt ttattttcta taatctcaat
     1381 taactctttc atcttagttt cagcttcacg attttctgct tctttttctg ctatcgtaag
     1441 ctttaaagac ttgatttcta attgaagctc ttcattagat gctccaacct tcttactcaa
     1501 ttcttcatta gctttaatca acttctcatt ctcatctgaa cattcaaata aaaggtctga
     1561 tatatttaat gcagttaata tcgcagcaac tgataagctc aatttcgggt tttgacgtgt
     1621 tattttatcc atttctttgt ctacaaaatc agcaatacta atcataaact tctctgattt
     1681 gtctccaacc attggatatt cagcgccatg gattttaacc attactttgt tcatcctaac
     1741 ctcccccgac attagttgtt aatttgatct taagttagca tttaattttt cgcttaattc
     1801 acttaatatc ttatcatgca ccttagctac atcttcatct gttagagttt tatctttact
     1861 tctataagtt attgaatatg caattgactt gtatccagct tctatttgag aacctttgta
     1921 aacatcaaat aacttgtagc tttctactaa tccttgacca ttttccttta ttatatcttc
     1981 tatttgtttt acaaatacat catctttaac aataagagct atatctcttg atgtagatgg
     2041 atattttggt aatggcacat aattctttgt tctatcagaa ttatcaaata caaagtcaat
     2101 atctatttca gcgacataaa ctctttgacc tagattgtag ttttctataa catctggatg
     2161 caattcacct aatgtaccta catatttatt attatacact attttagcac atctaccagg
     2221 gtggaaggta gtattattat cttgaggttc aatttcataa cctttaaatc cgacatttac
     2281 aagtatagtt tctatagttc cttttagtgc aaagaagtca acatctttac catacatacc
     2341 tacacacatt ctattttctt ccttaggtaa ttctgaatct tgtgggatga atatatgtcc
     2401 acactcaaat gcagatactt cttctatttt atgagataca ttagttgata aaacatctaa
     2461 catatttggt attaaagtag ttctcattac agaagtttct tctcctaatg ggttaagtaa
     2521 tttaacaaaa tttctctttg cattaccttc tggtactctt atcttatcta ctcccttagg
     2581 gcttacaaat gaatatgtta atatttcatt taatcctaca gcagttgaat tatcttttaa
     2641 agcatccatg aatttttgtt ttgaagtttt aattcctgct gtcgtatttc cttctaattg
     2701 tacagaaggt atattctcaa atccataaat tctagctatt tcttcccata catcagcttc
     2761 ttgttccata tctgttctaa agcttggtac atctatttct aatttatcat tagctaccaa
     2821 attacattta aactctaatg attctaaaat tcctacaaac tgctccattg gtacatctac
     2881 acctaataga tggttaattc tttgaggatt tactaccaat tttttaggtt ctggtttatt
     2941 tggatataca tctacaacac cttttaaaac ttttcctgct cctaacattt caacaagctg
     3001 tgcagctcta tttgctgcta tctctgcaag attagggtct aagtcttttt catttcttga
     3061 agatgcttct gacctaatac caactttttt agctgtcatt cttatgtttt ctggtttgaa
     3121 attggcactt tcaaaaagta cagtcttcgt attagaagtt atttctgagt tagcaccacc
     3181 cattacacca gctaaaccaa gtgatttttc tccatttgtt ataactagca tatctttgtc
     3241 taatgttctc tcaacatcat ctaatgttac aagtttctct ccatctttag catttcttac
     3301 tactattctt ccagtctcta cttgatttat atcaaaagca tgaagtggtt gaccaagctc
     3361 taacattacg aagtttgtta tatcgactat gttacttata ggtcttactc ctgcttctgt
     3421 aagtcttctt tgcatccaat atggagaagg ttctattttt acatctgtaa ccattctagc
     3481 aacatatctt ctacataaat ctggattatc tatctcaact ttgaaatcta tctcttcgtc
     3541 actttctttt acttcgattt caggatattt tacttttgtt cctatagttg cagctgcttc
     3601 tctagctata cccatcatgc atttacaatc aggtctgttt gaagttaatt caaaatctat
     3661 taaagcatct tttaatccta aaacatcttt tatatctttt cctaattcat aagaatcttc
     3721 gtggtctaaa atatatatac cacctctttt atactcctca atgtaatgtt catctatacc
     3781 tagttcagct gctgaacaca tcataccatc tgataattca cctctgaaat cagtctgttt
     3841 gatttcaact cctccaggta acttagaacc atttacagct actggaatat aatctccttc
     3901 tgatatattt gtagctcctg taactatttg aacaacttta tctccaatat ctactttggt
     3961 tacaaccaac ttatcagcat ttggatgttg ttttatttct aaaatctttc caaccaatat
     4021 attttctatt tcttcaccat aataatctat tgtttcaact ttagttcctg tcattgtcat
     4081 tttatcagcg aactctttta catccatgtc tatatcaaca tagtctctaa gccattttaa
     4141 agatactaac atctacatac cctcctaatt aaaattgatt taagaatctc atatcatttt
     4201 cgaataataa tctaatatca tctatttcgt atttaagcat tgcaagtctt tcaaccccaa
     4261 ctccaaatgc aaatccactg taaacttctg ggtctattcc acaatttcta agcacatttg
     4321 gatgaaccat acctgaacct aatatttcta tccaaccttc atatttacac attgggcaac
     4381 ctttaccacc acatttgaaa caagtaacat caacctctgc acttggttct gtaaatggga
     4441 agttgtgagg tctaaactta gttttgatat cagaaccaaa caatttttca acgaagatat
     4501 ccatagttcc tttaaattct gccatagtaa catcttttcc aacaacaagc ccttctatct
     4561 gatggaacat tggtgagtgt gtagcatctg gcgagtctga cctaaaacat ctacctggtg
     4621 caattacttt tattggtaac tcttgacttc tcatagttct tacttgaact ggagatgttt
     4681 gagttctaag taatacccca tcattgatat agaatgtatc gctcatatct cttgatggat
     4741 ggtctttagg agcgtttaat gcgtcaaagt tgttttcaac agtctcaact tctggccctt
     4801 ctgctataga gaatcccatt ccgataaata tatctgttac ttcatctata atttgagtta
     4861 ttggatgctt cttcccaacc tttaaaactt ttcctggttg agtaacatct atcacttcct
     4921 cttttaattt tctttctttt tcaatagcat ttatttcttc ttttttagaa tttatgctaa
     4981 gttcaatgtt ttctcttacc tcattggcaa ccttaccaac tactggtctt tcttcagcag
     5041 ataatttacc catttcttta agtatggcag ttatctcacc tttttttcct aagtacttaa
     5101 ctcttaaact ttctacactt tctatgcttt gtgcttcttt tatttcagcc aaagctgctt
     5161 cacgtaaagc aagtaatttt tcttgcacac atatcaccct ttcgtttttt tgtataaaaa
     5221 aagcccctca atccctgtct agggacgaaa ggctatagtt tcgcggtacc accctagtag
     5281 tttaacaatt taaaaatcat caaaccacct ctaacaaact taacgcgttt ttgacgtcaa
     5341 agcctactgt aaaatttcag ctctgttact ccagagggaa cttctcatga cctcttagaa
     5401 aacactccca gcctaggtgt ttatctcttt actaagttta atcaaaatac tttcctcttt
     5461 cattgcattt acaatattat attgtttatt ttagtattat atcatttcca ttcaaattat
     5521 gtgactattt ttaatata
//
LOCUS       DAMJWU010000169         5519 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMJWU010000169 DAMJWU010000000
VERSION     DAMJWU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.174"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            44..160
                     /gene="rrf"
                     /locus_tag="PC460_003529"
     rRNA            44..160
                     /gene="rrf"
                     /locus_tag="PC460_003529"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(374..1558)
                     /locus_tag="PC460_003530"
     CDS             complement(374..1558)
                     /locus_tag="PC460_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2602377.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1931..4282
                     /locus_tag="PC460_003531"
     CDS             1931..4282
                     /locus_tag="PC460_003531"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2602378.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4304..4843
                     /gene="nrdG"
                     /locus_tag="PC460_003532"
     CDS             4304..4843
                     /gene="nrdG"
                     /locus_tag="PC460_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2602379.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAKEAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKQVFLVEQYMKDDLSIAE"
BASE COUNT         2150 a          624 c          888 g         1857 t
ORIGIN      
        1 aaacttatta aaaacttaat agaaatatta agcataaaga ttatgtggtt acaatagcag
       61 agaggataca cctgttccca ttccgaacac agaagttaag ctctctagcg ctgatggtac
      121 ttggtgggaa actgcctggg agagtaggac gtagccacgt aatttttttg ttataataaa
      181 tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata aaagacttat
      241 tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa gttgttgaat
      301 atatcttata ttacttacct tatatattaa ttatcattaa tacattatat ctaattacta
      361 ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta tctaatccag
      421 ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc ataccaaatg
      481 ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa aattctatag
      541 aaaaactatc tttatattca aatttctcag atactttaga aagatctata aatatataga
      601 atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt gatttaatta
      661 aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag caatctttca
      721 aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga tgtccttgta
      781 tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat cttaatccag
      841 tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta gcttcttcgc
      901 ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat atttcatctg
      961 ctaaaatata tattttattt tgtatgcata catctactat ttctattaat tcatcttttg
     1021 tataaacact accagtagga tttgaaggat tgtttattac aagaattttt gttttatctg
     1081 ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt ttagtatcaa
     1141 taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc caataaggct
     1201 ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt gttattgagt
     1261 tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta ttttcttcaa
     1321 taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa tcatatttag
     1381 tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt acattaaaat
     1441 cgggttctcc tatactaaga tttattactt taactccatt actttccatt tctttaactt
     1501 tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt gataacataa
     1561 aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat ttacataagg
     1621 aaatttgtag atatatacat taatatatat aatagcctac actttttgcc taaaattagt
     1681 gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta ctgttcaaaa
     1741 tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca aatttggtaa
     1801 tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga tttttttaga
     1861 cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata gtaacaggag
     1921 gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc catttaatga
     1981 agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa gagaaggaat
     2041 agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt ttttaaatca
     2101 taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag ttaaggttct
     2161 tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa ctgaaagaag
     2221 tagaataaga aattcaaaaa caaggttgat gaaggcaata gaggaaatta cctttgaaga
     2281 tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata cagcaatggg
     2341 aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac atatattaaa
     2401 accagagcat tcttttgctc atgataatgg agatatacac atacatgata tggatttttt
     2461 aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca atggtggatt
     2521 ttcaacagga catggctttt taagagagcc acaagatata ataagttatg gtgcattagc
     2581 tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta taccattttt
     2641 cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca taggaaattt
     2701 agctaaagca ttaaagttat ttaaaggaat tgaaaataat gatgtgataa agaacatagt
     2761 atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg aattgtatct
     2821 aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac ttgtaaataa
     2881 gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa catatcaatc
     2941 tatggaagca tttatacaca atctaaacac aatgcactct agagcaggtg cacaggtacc
     3001 attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg taactaagaa
     3061 tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt ttccaatttt
     3121 aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt atgatttatt
     3181 taaattatca tgcagagtat cagctaaaag actattccct aattttagtt ttttagatgc
     3241 gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaagcta catatatggg
     3301 ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg gtagaggaaa
     3361 catatctttt actacagtaa atttaccaag attgggtata aaacatggta ttataaataa
     3421 tgaaaaggct aatttagacg gtttctttga agaattagat gagaagataa acttaattat
     3481 agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga attttccatt
     3541 cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag atacactaaa
     3601 agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag agtgccttat
     3661 tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag gattaaaaat
     3721 agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat taaatttctc
     3781 cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag ataaaaaagt
     3841 ttatggagaa attaaaggta tcacagataa agaatattat actaattcat tccatgtacc
     3901 agtttattac aatataagtg catatgataa aattgagata gaagctccat accatgaatt
     3961 gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg ataatttaga
     4021 agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg gaagtataaa
     4081 tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa gtaatatatg
     4141 ccctgtatgt ggaagaaatg aagatgaaag tgatatcaaa ttcgaaagaa taagaagaat
     4201 aacaggatat ttagttggta cagttgatag atttaataat gctaaaaaag cagaagtaag
     4261 agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa taagaatgtc
     4321 atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg taatatggac
     4381 ccaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg atttatgtgg
     4441 aggattttat atggatacag aagaaattat aaataaagtc aaatcattga aattacaaaa
     4501 aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag aagaaatagc
     4561 aaaagaagct aaaataaatg ggttagatgt atggtcatat actggattta cgtttgaaca
     4621 actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa agcaaattga
     4681 tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa agtttagagg
     4741 gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaac aagtgttttt
     4801 ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa ataaacagga
     4861 ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa gcttgtataa
     4921 tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt atagtttata
     4981 ggtgttttat gtggattacg aatgatatag tattgataca ttatttaata gattcttatg
     5041 ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat ctagatatgt
     5101 tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt gtgaatatta
     5161 agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac taaacacata
     5221 agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat gtaatatcta
     5281 tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat aatgtggata
     5341 atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg gataatatta
     5401 taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt tgttgactct
     5461 aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca aataaagca
//
LOCUS       DAMJWU010000170         5368 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMJWU010000170 DAMJWU010000000
VERSION     DAMJWU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.175"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            128..1618
                     /locus_tag="PC460_003533"
     CDS             128..1618
                     /locus_tag="PC460_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2602380.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1898..3394
                     /locus_tag="PC460_003534"
     CDS             1898..3394
                     /locus_tag="PC460_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2602381.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3528..4442
                     /locus_tag="PC460_003535"
     CDS             3528..4442
                     /locus_tag="PC460_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2602382.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            4560..4751
                     /locus_tag="PC460_003536"
     CDS             4560..4751
                     /locus_tag="PC460_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602383.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            4974..5324
                     /locus_tag="PC460_003537"
     CDS             4974..5324
                     /locus_tag="PC460_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602384.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         2135 a          534 c         1023 g         1676 t
ORIGIN      
        1 caaaaaaaca ctatatatat tcgataaaat aaaatttaaa catataatat tttatgtata
       61 tattaataaa aggacatatt gtgttataat ggtatcgtat aaaatcattt acgggggtgg
      121 tattaaaatg tttcaatttg aaacacaaat acacaaatta agacatgatg ttttaactgc
      181 aatagctaga cttggaaaag aagataacct taccagagaa gcaatggata atattcagta
      241 tgaaataata aaaggtgaga aacctacata cagatgctgt gtttataaag aaagagctat
      301 aatatctgag agagtaaatg taagtatggg gttacaacca ggtcaagata agatagattt
      361 agatacaatg gatatagaag aagatgaaca aattatatat attttggaag ctgcatgtga
      421 cagttgtcca ataaatgaat ttacagtaac agatatttgt agaggatgtc tagcacatag
      481 atgtaaagaa gcttgtaaat ttggagctat aacacatgtc ggaggaatgg cctatataaa
      541 tcatgaatta tgtaaggctt gtggaatgtg taaaaaagct tgtcaatatg atgccatctc
      601 agaggttgtt agaccatgta agagtgtttg tccaactaat gccttaggat ttgatagaga
      661 aaatatgaaa gctatgatac atgaggataa atgtttaaac tgtggagcat gtatgtcagc
      721 atgtccattt ggagcaatat ctgataagag tttgatagct cctgtagcta gaaagttagt
      781 tcaaaaagag aaaatgtatg cggtggtagc tccagctata acaggtcaag ttgaagcaaa
      841 tgttacatat ggtcaaataa aaaatgctat aaaatcacta ggatttgttg atgtattaga
      901 agcagcttgt ggagcagatg cagttacagt tcatgaaagc ttagaatttg ctgaaagaat
      961 ggaaaaggga gaagctttta tgacaaattc ttgttgtcca ggatttgttg gttatataga
     1021 aaaaacatac ccaaatcatg taggaaaaat atcttctaca gtgtctccta tgatagctag
     1081 tggtagattt ataaaaagtc atgaaccaga tgctaaagtt gtattcatag gaccatgtac
     1141 agccaaaaag agtgaggcag ctaagaaaga attaaaagga gctgttgatt atgtacttac
     1201 ttttgaagaa ataatggcat tatttgaggc atttgatata gacttggcta agtgttcaaa
     1261 tgaagatata gatgatgcat caatttttgg aagaggtttt ggagctgctg gtggtcttac
     1321 aaaagctata gaaaattatc ttgctgaaaa aggtattggt gttaattttg aacctgtaaa
     1381 gatttctggt tcaagagaaa tcaagaaaac tatgactatg gctaatttag gaaagctaga
     1441 aggtaatttt atagaaggaa tgatgtgtga aggtggttgt ataaatggtt ctggtaaatt
     1501 cttatcaggt gcaaaggtta aagccacttt tgatagaaaa aattctcaat caactaagaa
     1561 aagtgtctta gcaaatacaa agataaaaga ttttagtaat ataaatttag aaaaataaag
     1621 ataatacaaa gcaacaaaaa tacatattgt taatagagtt gttctaagtt gaggtttaac
     1681 ttttggacaa ctctattttt atttatatat ttaattattg aacaactaaa ataaaaatta
     1741 tatacactta aaataaaaat tacatatata atattttgta tataacttaa caaagtatat
     1801 gcctatgtta taataattta tagaaataaa taaaaaatac aatatacata atagtagaaa
     1861 tatagcaaaa aggtgtgatt tatagggagg attacttatg tttgaatttg aaacgcaact
     1921 aaaaaggtta aaacatgaag ttttaacagc agtagcaaga ctatctattg aagaaaattt
     1981 aacaaaagaa aatatagaaa aaattccata tgaaataatt aaaggagaaa aggcgagata
     2041 tagatgttgt gtttataaag agagagaagt agttcttgaa agggcgcaac ttgcattaag
     2101 tttaactcca aatagtaaat atggagatat aaatccagat tcagttagtt tggacacaga
     2161 tgagcaaatt atatatatag ttgaagcagc atgtgataga tgtccaataa atgaatttac
     2221 agttacagag ctttgtagag ggtgtttggc tcatagatgt aaggaagcat gtaaatttgg
     2281 ggcaatttct tatataaatg ggagagcata tataaatcat gatttatgta aggcttgtgg
     2341 aatgtgcaaa agttcatgtc aatatgatgc tatatcagaa gttgtaagac cttgtaaaag
     2401 cgtttgtcca acaggagctt tggattttaa tagaaacact atgaaagcaa tgatacatga
     2461 agataattgt ataaattgtg gagcatgtat gtcagcatgt ccatttggag caatatctga
     2521 taagagtttg atagcacctg ttgcaaaaaa acttgcaaag aaagaaaata tgtatgcaat
     2581 agtagctcca gctataacag gtcaaataga tagtaatatt acatatggtc agttaaaaaa
     2641 tgctataaaa tcactgggat ttgtagatat gatagaagct tcttgtggag cagatgcagt
     2701 tacagttcat gaaagcaatg aacttattga aagaataaaa tcaggagata attacatgac
     2761 aaattcatgt tgtccgggat tttttaagta tatagagaag atgttcccag atgaaaaaga
     2821 tagaatatca tctacagtgt ctcctatggt agcaacagga agattagtaa aaaaaataga
     2881 ttctgatgct aaagttgtat ttataggtcc atgtacagct aaaaagagtg aggcaacaga
     2941 gaaaagttta aaagattcag tagattatgt acttactttt gaagaactaa tggcattatt
     3001 tgaagcattt aatatagatt tatcaaaatg ttcaaatgaa gatataaatg atggttcgat
     3061 ttttggaagg ggatttggag catctggtgg acttgccaaa tcagtacaga actatattgc
     3121 tgaaaagggg cttagttttg attttgaacc tataaaagta tctggtccaa gacaaattaa
     3181 aaagacaatg acaatggcta aattgggaag attagaaggt aattttatag aaggaatgat
     3241 gtgtgaagaa ggttgtatta atggagcagg taagttttta tcaggtgtaa aagctaaaaa
     3301 tacttttgat agaaaaaatg gacaatctac taaaaagagt gttttatcta atgataagat
     3361 taaagaattt gaagatgtaa atttagaaag ataattgttt tggtacagtc atttttttat
     3421 catgccacaa atatgcctaa aatttgccct ttttatatgt gtattttaca gagttaaggt
     3481 tgctatacta taaatatgta ataaataaaa tttataggag cttttggatg aggaaaatag
     3541 tattaaaaga tatcctgttt ttagttacta cattatctat aatagtaata ttattgcttg
     3601 taacaaatat aactactaat agtaagataa aatcaacgaa tgaatcttat ttaaaaagtg
     3661 acaaaaatga tgtgagtcaa aataatattc agagtagcac aaaaaatact gcatttaaga
     3721 ctactatatt aagtactgga aagtctgact gtatattggt tgaaataggt aacaaagtca
     3781 taatgattga tacaggagaa gataaaaatg gaaaacaaat agtggatagg ttaaaagaaa
     3841 aaggaataaa tacacttgac tatttaattt taactcattt agataaggac catataggtg
     3901 gagtagatag tgtgttatct agtgttaaag taaagaatat tatacaagca aattataaga
     3961 aagatagtaa gcaatatgat gaatatattg attcacttaa aaaagctgat atagaaccag
     4021 tgctactaaa agataatatg aatattgtaa tcaatagtgc agaaataaac atacatccgg
     4081 cttcaaaaag caagtacgag agttcaaatg attattcaat aatcactaat ataagctatg
     4141 gagcacataa gtttttattt gcaggagatg cagaggaaga aagattgtca gagtttataa
     4201 atggaaatac tttaaaatat gattttgtta aaatgcctca tcatggaaga tatgacaaat
     4261 taactgaaac atttttagag tctatttcac cccaatatgc tgttattaca tgttcagaga
     4321 aaaaagaacc agaggaaggt gttttaaaaa tattggaaag attgaatata aaaacatttt
     4381 tgacttctaa tggagaggta gttatcaata gtgatggtaa gacattgagt gtgaatcaat
     4441 aattttttta aaaatatcta aaaaaattta aaaaaatcat tagcaaatat taccccttaa
     4501 acatagtata ttttaggagc tgaaactcca catacaaaat gactaggagg taacatatta
     4561 tgttggatag attttttggt gaaggcggat ttggtggatg tgcttggtgg ataataatat
     4621 tattcttctt attcttagca ttccaagatt gttgggctga aatggactta tgtgcatgga
     4681 tacctttctt aatattatta ttaatacttt gtgaatgtgg tgacgttttt gatggaggat
     4741 gtggatgcta aaaaaatcaa tttagatgat ttaataaagc ttatggtggg tataaataaa
     4801 ataccaaacc aatcacaatc tagtgtttga aggtagtcgg aaatcccccg ctaccttctt
     4861 ttttttattg ttaaaattta tttttgaatt atttcattta catataataa tttttacgat
     4921 aaagtctgat ataattaaaa agaaaataaa atttatttat aggagatgta aaaatggcta
     4981 tagtggataa gattttcaat gagataaaac ttatggttgc aagactttta ttgggaaaaa
     5041 aatacagtga atatgaagaa aaagatttag tgtataatac agaagaagaa gttatactta
     5101 taactttaaa aagattagtt tttcagggaa atgtaaacga agcagaagaa attttatttg
     5161 ataaagctaa gagtgtaaat agtgaaaata tgcagtatat agcaatagaa ttttatacta
     5221 tgcttatgga aaaaactgat gaagaattag aagctatgaa tttttctaaa caagaagttt
     5281 atcaaggtat agaagatatt agaaaagtat tgaatttaga ataaaagata tatgaaaata
     5341 ttgaaataaa ttaatgctaa ggtgagaa
//
LOCUS       DAMJWU010000171         5359 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMJWU010000171 DAMJWU010000000
VERSION     DAMJWU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.176"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            219..1682
                     /locus_tag="PC460_003538"
     CDS             219..1682
                     /locus_tag="PC460_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2602385.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1738..2874
                     /locus_tag="PC460_003539"
     CDS             1738..2874
                     /locus_tag="PC460_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2602386.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3110..4225
                     /locus_tag="PC460_003540"
     CDS             3110..4225
                     /locus_tag="PC460_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF2602387.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4377..5246
                     /locus_tag="PC460_003541"
     CDS             4377..5246
                     /locus_tag="PC460_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2602388.1"
                     /translation="MDYLKYFKKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         2068 a          545 c         1003 g         1743 t
ORIGIN      
        1 ttcaaacaca cgtcgttaaa aattttataa atttatgttt agatatgatt aatactcttg
       61 attttagaaa taaatatttg tagattgtag attaaagata atataaataa atagaaggta
      121 gattttttag atatattaat ataaagatta tataaaatat tgaaatatag ctgtttaaat
      181 tattgtctat aaatataaaa tgaatggtgg tgagttaagt gaccgattta gttaacacca
      241 ttaatggaat tgtttggtca cccgtattag ttgcaatgtg tttgtgtata ggattatatt
      301 tttcattgaa attaaaattt tttcaaataa gagatgtaaa agaaatgttt actctcttgc
      361 ttgaaggaga tagttctcat gttggtatat catcatttca aggttttgct acatctatgg
      421 ctggaagaat agggactgga aatatagctg gtgtagctgt agcaatagca atgggaggtc
      481 caggagcatt agtatggatg tgtataatgg ctacattagg ttcagcaact gcatttgtag
      541 aatctactct agctcaaata tataaagatg agcatgatgg gcagtataga ggaggacctc
      601 catattattt tgaaaaaggt cttgggtgga aaaagtgttc agttttattt tcaatagttg
      661 caataataag ttatacattt tttatgcctg gtacacaatc aaacacttta tatctagcaa
      721 tgaatcaagc atttggattt tcaaaaatga caatagcagt agcatcttct attgcattag
      781 cgataataat ttttggagga attaaaagga ttggtttatt tgctgaaaaa gtagtaccta
      841 taatgggtgg agcatacctt attatgacat tagtaataat tataacaaat attagtttag
      901 ttccagaggc tattgtaaca atgtttaaat ctgcatttgg atttggttca gtttttggag
      961 gaatgctagg ttcagcaata tcttggggag taaaaagagg tatatactca aatgaagcag
     1021 gagaaggttc tgggacttat ggtgcggcag cagcagaagt gagtcatcca gccaaacaag
     1081 gattagttca agcattttct gtttatgtag acacattatt aatatgtatg gctacaggta
     1141 ttatgattgt aataacagga atgtataatg ttacaggtgt ttgcacagca ctgccaggtg
     1201 tagaaccggg accacaatat gtacagagtg caataaatac attacatcca caattaggtg
     1261 atatttttat agcaatagtt ttattattat ttacatttac aactttatta gccttctcat
     1321 atatgatgga gactaatgta tcttacttag taactagaat taaagcagaa aaacatgaaa
     1381 agtacataat taatgtctgt agatttatgc ttatagcatg tgcagcaatg tgttgtttta
     1441 tagaaccact tacaagctgg gcattaggtg atatatgtat aggtgtatta acatatataa
     1501 atttaattgc catctttaca ctaaaaaaac cagctataaa ggcatattat gattatataa
     1561 gacagaagaa gatgggaata aaaaggagtg atagaacttt taaccctata gagttagata
     1621 taagaaatgc tgagttttgg gaaaatagat acttaaaaga ggataagcat gagttaaatt
     1681 aaagttataa taaaatataa aaattactaa ataaatttag tgggaggtaa gattggtatg
     1741 aaagaaagaa taatacaagt ggctgattca aagaaagaaa aaattctagg gctatgtcaa
     1801 tctttatatg atgaaccaga aattgctttg caagaatata aatctgctaa aaaaatatca
     1861 gaatttttaa gagaagaggg ttttgatgtt gaggaaaact tggcaggtat ggctacagct
     1921 tttaaagcaa ccaagaaaaa tggagatggt cctaaaatag catttatagc agaatatgat
     1981 gctcttccag gcaatggaca tgcatgtgga catcatttaa ttgcatctat gggagttgga
     2041 gctggaatag ctctttcaag tatactggat acttacaagg gagaagtaag tataattgga
     2101 acacctgctg aagaaactgg agatggaaag ccttacttga tagaacatgg tgtatttgat
     2161 ggctatgatg cagcaatgat gatacatcca aattcaaaaa cttgtgtaac acctgaaatt
     2221 atagcaatag gaggtctaga tttcatattt actggaaaag catctcatgc aggagctaag
     2281 ccatataacg gaataaatgc tttagatgca gtagtattac tatataataa tataaatgca
     2341 ctaagacaac aattagtaga tggaacgaga atacatggta ttatactaga agcaggaact
     2401 gctgcaaatg taatacctga tatgggaaaa gtaagattag agataagggc aaaagaacaa
     2461 aattactttg atgaagtagt agaaaaagtg aaaaattgtg ctagaggtgc tgcaatagcc
     2521 actggatgtg aacttgaatt ttatcacttt gaaccaactt gccaaggatt aaatgaaaat
     2581 aaagttttgg tagacatatt tacaaagatt atggaggaat ttggaatata tgaagatgaa
     2641 caagtaatgt taggttcaac agatatggga aatttaagtc aaataatgcc atgtatacat
     2701 cctttgatga agttttctga aaatggagaa gaactacata caaaagaatt tttggaggct
     2761 tctataaatt catatgcaaa agatagagta attgatggta taaaaatatt agctttaaca
     2821 ggatttaatt tatttgaaaa tccagagtta ttgaaaaaaa tgaaggaaga gtagaactta
     2881 gactattttt aattatactc tttaaaataa gaagagtgtc tcaaaatgaa ctttttagtt
     2941 tatgagacac tcttttttgt atgaaattaa atatattttt attattttaa caatgaaaaa
     3001 gaggcttatc tgtttttata tgaccttttt taacttgtag cacaaaatat catgaatgat
     3061 tacaattcga atgataaaat cgattataag atatatagga ggggtacata tggaaacttt
     3121 agaagtaaga aatattgaat tgagtggtac aaattatgag ataggataca gactaggaga
     3181 attggttgct aatatgccag agataataga aggtcagatt aataaatcta atctggtaag
     3241 caaaaaagaa gaaaaagaga tgatagagtt gtttgataaa tattgtccag gtcttaatga
     3301 agaattgcaa ggttttgctg atgcaattca agtgaattgt aatcaaattt tatattatac
     3361 tatgacttat cttaaaccag gttgtagtca agttgcatta gcaccagaat taactgaaaa
     3421 tgggcatgta ctatttgcac gtaatttcga tttctcacac aatatggagg attttgtttt
     3481 atgtaaaact aaggtaaatg gaaaatatgc acatataggt actaccgtta tgcaatttgg
     3541 aagaggtgaa ggtatgaatg aatgtggctt aggtgtaagt cagtcatcat gtggtattcc
     3601 tgtaggcaat tcagatggtt taagaaaacc agcaattgta ggtttacagt tttgggctgt
     3661 tattagatat ttgttagaaa attgcaaaga tgtagatgaa gcattagaat atttaaaaga
     3721 tatgccaatt gcatacaata ttaatttatt gcttgcagat aagtctggaa acattgcact
     3781 tgtggagact ttagatggga aaaaagaagt aaatataatc aatagtttag aaagtaaaag
     3841 agaatatttt ttacactcta ctaatcatac acatattgat aaacttcata aattagagcc
     3901 acaatcaatg aaaaattcta ttcatagata taaattaatt aaagaatata taaataagtc
     3961 taaaaaaatt ggagagaaag agttaatgaa tttattgtct tcaaaatatc caaatggctt
     4021 atcttgtaat tactataatg atttttttgg aactttaaaa agtatagtta tggatttaaa
     4081 tataggaaag tttaatattt tatggggtgg actagagaat aaatgggaga gctactattt
     4141 aaaaaatgac attaaatcta taacacagag gataaacata aatattgaaa aagcaccatc
     4201 tgactttttt gatttcattg aataaaaaat gtatttaatt attctaaata attaggatat
     4261 ataaatcaac taaaagtttt tatttatcgt attgaattag ttataatatt ttagtatagt
     4321 aaaaaatagt gctattttac atatttaaag agtttaagta gaagagaggt gccatattgg
     4381 attacttaaa gtattttaaa aaagctgtaa tgtatattga gaataattta catgaaaata
     4441 taactgtaaa tgatgtggca aaagaaactg gatattctta ttatcattta acacgcttat
     4501 ttaagtctat gtttggtgag agtgtaggaa gttatataaa aaaaagacga ttagtaagta
     4561 gtacaaaaga acttttatat agtgataaaa aagtgataga tatagctatt ggtagtggat
     4621 ttgaatcttc ggaagcattt agcagagcat ttaaatctat atacaaggtt agccctatag
     4681 aatatagaaa aaatagaatt gatgtatttg ttggtaaaaa gaaaaaatta gagttagatt
     4741 ttatgagaca tcttgttgga aatataacga ttaaaccagt tataaaagaa atagaaaaaa
     4801 taaaggtaat aggtataaag gataaagtaa ttttagaaaa taattcatta cctgacttgt
     4861 gggagaaatt cagaaaagta catcatattg tcccaaatac tttgccatca aagagagttt
     4921 ttggaatctg tgaagcaatc tctgaaatac acctagttag tgaaagtatg gaatttaatg
     4981 agataatagg acttgaagtt aatagttatg atgtgattcc aaattcattt gtttcaaaaa
     5041 ctattaagga aggaaagtat gctatattta ctcatactgg tagcttagat aatttagaca
     5101 aaacttatga atatatatgg ggtacttggt ttttaagttc aaaagaagaa cttgatataa
     5161 gagatgattt tgaagtatat gatgaacgat ttttaggtcc tagtaatgtg aactcacaaa
     5221 ttgatattta tatacctata aaataatcta taagaaaact aaaaaataat ttgattaaga
     5281 aatagatgtt cgtaaaagag catctatttt ttatttttat cagatttaga taattttaat
     5341 aataaaagaa ataaaaata
//
LOCUS       DAMJWU010000172         5350 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMJWU010000172 DAMJWU010000000
VERSION     DAMJWU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.177"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            301..1827
                     /locus_tag="PC460_003542"
     CDS             301..1827
                     /locus_tag="PC460_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2602389.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1855..2412
                     /locus_tag="PC460_003543"
     CDS             1855..2412
                     /locus_tag="PC460_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602390.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2934..3977
                     /locus_tag="PC460_003544"
     CDS             2934..3977
                     /locus_tag="PC460_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602391.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4088..5230
                     /locus_tag="PC460_003545"
     CDS             4088..5230
                     /locus_tag="PC460_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2602392.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2262 a          444 c          939 g         1705 t
ORIGIN      
        1 atattaaact aactaaatca ataaaaaagt tggtggataa caatttataa ttgttagcac
       61 aacatcatct tatgtattga aatctaaaat atcctcattt ttacttagca taaatactat
      121 taattaagaa aataatatag aaattataaa tatatataag tttagtctgg tgtataaaac
      181 aagaaaacta agtgattttt aatttaactt tttattaagc ttatttttta tactatttat
      241 aataaataaa ctccatgatg ggaaaactat tactatttaa tatgtaaagg agtattttaa
      301 atgaatgaat catattggtt tttaaactca agtccaaaag aatataacaa attaggtgaa
      361 aatataaaaa cagattgtct tatagtaggt ggtggaataa ctggattgac aactgcatat
      421 ttattagcaa aagaaggtaa aaaagtagtg cttgttgagg ctgataaaat aggatatgga
      481 acttctggaa ggaatactgg taaggtaact tgtcagcatg atatatttta ctctaagata
      541 gagaaaaaat atggattaga taaagcaaaa tcatattata atgcaaataa tgaagcttta
      601 aatttagttg aacaaataat agaagaaaac aatataaaat gtgattttaa aagggaaact
      661 tcttttgtat ttacagaaaa agaggatact ataaaggata ttaaagatga atatcgtaca
      721 tgtaaaaaaa taggaataaa ttgtgagtat catgaaacta tagaaaatat tccattagat
      781 ataaaggggg ctattagttt tactaatcaa ggtcaattta acccaaagaa gtatattgat
      841 ggattggcaa aagcagcagt aaatttaggg ctaaaaatat atgaaaatac accagtagta
      901 gaccttgaaa aaggaaaaat atgtagggtt aaaacaagag aggataatat aatagaagct
      961 gagaatgtta taatttcatc tcatagtcca tggtatgatg gattgaattt ttattttgca
     1021 aaagaatatg cagagcgtgc ttatttaatg gttgcagttt tggaaaataa attagcagat
     1081 ggtatgttta taagtataga tgacccatca ataactttta gacaatataa tgatggaagt
     1141 gaaaatttac tgatttttgg aggaggagac cataaagttg gtcagggagg aactgagaaa
     1201 gaaatatttg atgatttaga gcattatggt aaagaagtct ttaaggtaaa agattttaag
     1261 gggaaatggt ctgctcaaga caatatgagt tttgacaatg ttccttatat tggttatatc
     1321 aacaaaagag aagacaatat ttatgtagct acagggttct ctaaatgggg aattactaat
     1381 ggaactgctg ctggaataat aataaaagat ttgataataa ataataattc tgattataaa
     1441 gatactttta atccatccag attaggtagc tacttttcaa aagattttat aaaggaaaat
     1501 gcaaacgtag caataaatta tgtaagtgga aaattaaaaa taggtagtgg agatatgcct
     1561 aaaaataatg gagaaggaaa gattgttaat attgatggaa aaagatatgg tgtatataag
     1621 gatgataatg gagattttta tattgtagac actacatgta ctcatttagg atgtgaactt
     1681 aatttcaaca gtgaagaaaa aacatgggac tgtccatgtc atggttctag atttgactat
     1741 aagggaaata tacttgaagg accagctctt aagccattaa aactttatgg acatggtgat
     1801 aatgatgtta atcctaagtt attataaaat tatttttagg aaggtaaagt gtatatgaat
     1861 aaacttttaa agccaataac tatactattg tatatagttt atattttatc tgccgtattt
     1921 tttgtagcaa acaaagaata tggaagtcta ggtctggtag caattagttt agttggtact
     1981 ttcattttga gttttataaa taagcaaacg gaaagattat tgagtaaaga attatatata
     2041 atgcttgctt tatttataat gttttcatca ttgttaggta cttgttttaa tttttatgat
     2101 ataaattatt atgatgattt tttgcattta tggtcaggta taatatcttg tattgtggct
     2161 ttttctataa ttaaatactt ttatacacaa agagagataa gtaaaatgag taaagtgttt
     2221 ttagttatat ttttatttat gtttagtatg ggagtagcga gtttatggga aataggtgaa
     2281 tttttaatgg atacttttgt tgggactact acacaggccg gagggttaga agatactatg
     2341 attgatatgg tagatgcttt aataggcaca attataacta ttccttttat agtaaaaaaa
     2401 tctaatatat agttttaaat tttttatatt aattataatg gatttaatta atcatgttga
     2461 gtttcaaggt ctatatgatt aattaaatcc atttttgtta tttataatta ttattgaata
     2521 tatttttaca tataagatat aaggtgatag atagaaattg ttggattatg taaataatta
     2581 atatataata ccattatggg caataaatac aaatcaaatg ataatatagt ctatttatgt
     2641 aaatgttata ttgtatacta agtataatag taagtttttt gatagtggtg tagaaagtaa
     2701 aaatctacta gcctaaattg atgtatggat tagtttagga acagtaattg catatcttaa
     2761 gtttgatgtc atgttgggct actagaaata ggtcatcttt ttcacttgaa tatcttttct
     2821 ttaaacatgg agagtatcat atcaaaaaca tatgaaaatt aagtgattta taagaacagt
     2881 aatattttat atgatagtat agaatagtaa taaagtatta ggaggaacat tgagtgttaa
     2941 aaaagtggtt tggtattgtg aaaaaaagac aaaaaagtga gtcagcaaaa gaagaaggtg
     3001 aagtaatatt aaaaaacgaa aaaatattat ctgaagaaaa gttgatagat gaagaaggag
     3061 ttgtagttaa tattgataat gaaatattaa ctaaagtaga agtagtaaat gatgataatg
     3121 aaatagaaga aaaaataata gaagaagatt ggttaattag tgaaaatacc ataaaattag
     3181 atgataaaga agcaattatt aatgataaaa acatagaatt atgtaaaaaa gaagttcaag
     3241 ttgaaagtga aaaaataggt ttaaataagt ttgaaggact tgaccaaaat gaaaattata
     3301 atctagaaaa aaatgttatt gaagaaaaaa aagtaagtga atgtttgaca gaagaagatt
     3361 tagagtacat aaaagaaatt aaaataaaaa gaggaaaaag tataaaagct ataaacttgt
     3421 atactaaaga agagtgggtt tttgacactc atatacagtg tagtaaaaaa ctcaaagttc
     3481 cattagggta cataagagaa aatttaaaat atggatatat ggattacttt ggagatgcaa
     3541 taaattattt aagtgaagta ttaaatatag atgaatactg caaaagtgaa tggagctatc
     3601 tcgataatag caaatctcca tctgagatat ttaatattct aaacaataaa atatttagta
     3661 taaggctttc taatgaaaaa agaaatgaaa ttttgacaaa tgataaaatt gaagcattaa
     3721 aaatgaatta tagatttgaa tgtattgatg aagaatatga tgaatatttt aaaaaatata
     3781 agtctataat caaaagaggt ggaaagaaaa aagttgaatt agtaaataaa aaaggtgaca
     3841 ttttagaaat atttaagtct ttagaagaat gtgccattta tttgcaaaag gaaaagaatg
     3901 aagttataca aatgttaaaa tatggagata caaaagtagg aagaaacttt ataaggtata
     3961 gtttgagaag tatttaattt cattttaaat tcataagagt ttcatttctg attcacacat
     4021 ttaatattat tttaatgtaa aataataaag gtgaagtatt gcacaattaa aattaaggag
     4081 ggtattaatg tctgaagaaa aaaatccaaa agaaaataca ttagatgaat ctaaaaattc
     4141 agataatgta aattctaaaa aagatgatgt gtcagatgaa aaaaatactg ataacaaagt
     4201 taaaaaaagg gaaataaagc taaacaacaa aacacttaaa gctattgtag caattgttgc
     4261 agtaggagct atatgtttag gtggaggcta tgtttttggc aaagaagctg gaagaaaatt
     4321 gcctgctact tcaaaacact ataatagcag tgaggtagta gctactattg gggataaaaa
     4381 aataacaggt gaaatgttaa gaaagagaat ggagccatta ttttatttaa atgcaaaaac
     4441 tactatgact gatgacgaaa tacgttcata tgaatcaaat atgattgaaa ctatgattac
     4501 atctgatata ttatcctctg aagcagcaaa agagaaaata agtataacta atgaccaact
     4561 agatgctcag tataataatt taatgagtag tatagaatca gcaatgggaa tgactaaaga
     4621 agagttttta aaacagtttg atttgactga aaaatatata aaagatgata tgaaaaaaga
     4681 gctagtagca tcaaaatatt taaatgaaaa atcaaaagtt actgataaag aagcagagaa
     4741 ctattacaat aaaaacaaga gcaattattt acaagttaga gcttcacata ttttaataaa
     4801 aactgttgat gataaaggta agcaagttag tagtagcaaa aaagctgaac ttaagaaaga
     4861 agctgaagaa atacttaaaa aagcccaagc tggggaagat tttgctactc tagctaaaaa
     4921 atactctgaa gatagtagtg cagaatcagg tggagattta ggtttctttg gtaaaggtca
     4981 gatggtggaa tcttttgaaa aagcagcctt tgcattgaag aagggagagg tatctaacaa
     5041 acttgtagaa agtgattatg gatatcatat aattaagaaa actgatgaaa aatatcagcc
     5101 atttgaagag gtaaaatcag atttagtaag cagtttaaca tcagaaaaac aaaacctact
     5161 tatacaaaat ctaaaagaaa aatataatgt taaaattaca gataaatatc aaatgaaagc
     5221 taaagaatag ataaagtcaa tttattaaaa aaaagaaaaa ttaactgtat ggataaaagc
     5281 tatttttact cttaatatag agagtataaa atagctttta ttattttgca ataaaatcta
     5341 tttatatata
//
LOCUS       DAMJWU010000173         5328 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMJWU010000173 DAMJWU010000000
VERSION     DAMJWU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.178"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            100..936
                     /locus_tag="PC460_003546"
     CDS             100..936
                     /locus_tag="PC460_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2602393.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1289..2866
                     /locus_tag="PC460_003547"
     CDS             1289..2866
                     /locus_tag="PC460_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2602394.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGIANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2859..4043
                     /locus_tag="PC460_003548"
     CDS             2859..4043
                     /locus_tag="PC460_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2602395.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            4061..4660
                     /locus_tag="PC460_003549"
     CDS             4061..4660
                     /locus_tag="PC460_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2602396.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4684..5154
                     /locus_tag="PC460_003550"
     CDS             4684..5154
                     /locus_tag="PC460_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2602397.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1989 a          561 c         1022 g         1756 t
ORIGIN      
        1 caaaatatat acgaaagtaa aattttaaat attgacatta aattaaatat actatactat
       61 gtaagtgtga tgagagaatt tttttagagg ggatttatag tgaatataaa aaagatattt
      121 aataacaatg tagtagtaag ctccctagaa gatggcacag aaataattgt cactggtgct
      181 ggagtaggat ttaagaaaaa agtaggcgat ttgatagatg aaaatttgat ttctaagaaa
      241 tattttgttc aagacgatca aagggacaaa tacaatcaaa tacttaataa gacctctatt
      301 gaatacttta aaatttcaga agaaattata gaaaaggcta atgaagtttt aaatactcaa
      361 gtgaatgatt caataatatt agccttaact agtcacatag aatttgctgt ccaaagagaa
      421 aaacaaggta taaaattacc aaacttaata ttgaatgaga caaaacaact ttatagggaa
      481 gaatttgaat ttggattatg ggcaattgat gaaattgaaa aaaggatagg aataaaattg
      541 cctgaagatg aagcaggata tattgctatt cacataatca atggtttgga aaattctcaa
      601 aaagatgaag gtattaatat actagagttt tcaaaacaag ttattcagat aattgaagaa
      661 gtacatggat ttaaattgga tgtaaattca ttaaattata ctagacttat aactcactta
      721 aagttttttg ttcaaaggat tctccgaaaa gaaacttaca atgatagtga tattgaagat
      781 atgtataagt tagtgaataa aaattacaat aaacctaaag tgtgtactga agaaattgca
      841 gcattggtat tagacagatt tgaatataag attagtaaag aagaagaatt atatttaatg
      901 attcacataa ataaaataac aaacagtggt tgttaggatt gtaactgata aagcaggcaa
      961 aacctaagtc attgagctta actattagat taagtttttt gatttaggtt tttttattag
     1021 atacaatcaa aatacagaca gtgactttta ggattgtaac tggttaagca ggcaaaacct
     1081 aaatcattga atttaactat taggttaagt tttttgattt aggttttttt attagataca
     1141 atcaaataca gacagtgact tttaggattg taactggttg agcaggcaaa acctaaatca
     1201 ttaaacttaa ttattaaatt aagttttttg atttaggttt ttctataaat ttgattatgt
     1261 ttttagataa aaataggagg ggaaaattat gaaaaaaaat aagttaggtt tatggaactt
     1321 cttccaaatg ttaggaaaaa cgtttatgtt cccaatagca ctacttagtg tttctgggat
     1381 gatgctagga cttggggcag gttttacaga ccctaagatg attgaaatgg ttccattttt
     1441 agggaatgaa tatgttaact tagttttaaa cttcatgtta acaataggat tatttgcatt
     1501 taacaacttg ggagcattat ttgcaatggc aattccacta ggattattaa aaaaagaaaa
     1561 agagtttggt gctttttcag gattagtagg atttatagca atgcatatag gtacaaattt
     1621 ttatttaaca agagctgatt tgttagtacc agcagaacag atgtcaacta atggtcaagc
     1681 tatgattatg ggtatacaaa catataatac aagtgtttta ggtggtattg tagcaggatt
     1741 aattgtttat gcaatatatg ataaagttag taatttaaag ataccagaat cattaggatt
     1801 ctacagtgga ccaagattag ttccaattgt tagtttaata gttatgagta tagttggatt
     1861 attaatacct attgtttggc caccattttt caatgcattc caaagtttag gaagatggat
     1921 ttcttcagca ggaccattag gatatttttc atatgcagtg gcagaaagag ttacaattcc
     1981 atttggacta aatcatcttg taacatctgt attccgtttt acaccaatag gtggaactgc
     2041 tgttattgat ggagaaacgt actttggtac attgaacatg tttatggcat atgtagaaaa
     2101 taatcagatt atacctcttg atttagctgg taaaatggaa caaggtaaat tgatgattca
     2161 atatggatta gctggtgctg cattggctat ctaccgtaca gcaaaaccag aaaatagaaa
     2221 ggcacttaaa ggacttttaa tatcaggagt gttgacagtt attataggtg gtataagtga
     2281 accaatagag tttctattct tattcatatc accagcatta tttgctttcc atacatttat
     2341 gaatggtata gcaaacatgg tacttccata cttaggcgtg ttgatgggat tcacaggaga
     2401 tttaattgca tttataagtt ttggtgtgtt aagaggaaca gctacaggat ggccaatagc
     2461 agtattagta gcagttttat attttgcaat atactatttt gtatttaagt gggcaattat
     2521 taagtttaat ataaagaccc caggacgtga ggataaagaa ataaaagtat ctgaaggaaa
     2581 tggttcagat ttcaaaaatt tgtctacata taaaggacaa caaatgatat ctgctttagg
     2641 tggtcaatct aatatattat cactagataa ttgtgtaact agattgcgtt taaaactaca
     2701 agatgtatca ttaataaatg aggatgcaat taaagaggct ggtggtatag cagttgttaa
     2761 attagatgag catacaatac aagtaattat aggaactcaa gtgtattctt taagaaagca
     2821 gatggataaa gctatggaag gtcatttgga ggcatgtaat gattaatttt gataaaaaag
     2881 tagaccgatt gggtacatat tgtacccagt gggactatgt agaagataga tttggtaaga
     2941 aggatttgct tccttttaca atttcagata tggatttaga gtttccattt gaaataacag
     3001 aggtgttgca ggaaaggtta aatcatagag ttttgggata cagtagatgg aaacatgatg
     3061 attttagaaa ttcagttacg aattggtata ttaaaagatt taattgtagc gttgatagta
     3121 actggatata ttatagtcca agtgtaatgt attcaattag caaacttatt gagatgttaa
     3181 gtaatgaagg agatggaatc cttataaata ctcctgcata caatgctttt tatgatgtga
     3241 taataaataa caatcgtaat attataaagt cacatcttgt aaataggaat ggattctatt
     3301 caattgattt taatgatttt gagaaaaagt gtaaaaaatc taagatattt attttatgta
     3361 atcctcacaa tccaactggt agagtatgga gtgagttaga acttgttaag atgataggaa
     3421 tttgtaagaa taatgatgtc aagataatat cagatgatat acacatggat attgtatatg
     3481 acaatagaat gacacctatc ttaaatgttg caaaggacta tttagattct gtattcattt
     3541 gtacatcagc gtctaaatct ttcaatatac cagcattgac tgggtcatat gtgattatac
     3601 caaatgaagg tattagaaat aaatttgaaa atatcactag ataccgtgat tttgtaaatt
     3661 cgccagcaat tctagcaata ttagctacaa tggtctgtta tgatgactgc gaatattggt
     3721 tagatgaact attaaaatac cttaaagaga atcttaaata tacgattgat tatataaaag
     3781 ataatttaga accattgaaa ttggtcatgc cagaaggctg ttactttgct tggatagatt
     3841 tttccgaact taaaataagt ggtgagaaat tccaaaaatt attgatggat attggagaag
     3901 ttgctattat gtcggggtct gtatatggtg aagaaggtga gttttattta aggttaaatg
     3961 tggcatgttc aagagaaaaa cttgaagatg gtttaaatag gattaaaaaa actatggatt
     4021 atattgcaaa aagaatttta tagtaggaag ggagatagtt atgggatatt taaaaacaat
     4081 ggttgatgtt atgactactg aaatgaatgc cataagatgt ttaatagatg aagcaggaat
     4141 agaatatgaa agtatagtta gtgaaattgc aaattgtgaa ggcaaagtaa tttttatggg
     4201 tgtaggaaaa tctgcacata taggtaagaa attggcagct acatttgcta gtactggtac
     4261 acctagcttt tttgtacatg caacagaggc agtgcatgga gatttaggaa tgattgaaag
     4321 taaggatatt acaattttaa tatcaaatag tggaaattct atggaagtgg taaactgtat
     4381 aaaatatatt aaagcaatag gttctaagac aattgcattt acttcaaaca gaaattcagt
     4441 actggcaaaa gagtgtgatt atgctttaat atatcctgca aaggatgaag cagaccattt
     4501 aaatctagca ccaacaacat catctacaat tactttggtt ttaggagatt ctattgcatg
     4561 tgcattatct aagagtagta attttggttc ttcagatttt tataagtacc atccaggagg
     4621 aagtttaggt gagaagttaa aaacagctaa caatggataa aagcaaaaag gagagtagtt
     4681 tttatgttca aaatgtttaa aaaaaaatca ataaaatcac ccatatcagg aaaagtaata
     4741 gagttatcta aaatacaaga tggtgtattt tctcaaaaaa ttatgggaga gggtgtagct
     4801 atagattcta caggagacat tgtatatgct ccaatcaatg gaaagatagt agtagtagca
     4861 gagactaaac atgcatttgt aatagaatta gaaaatggga tggaacttct tattcatgtt
     4921 ggtttagata ctgtaaattt aaaaggcgaa ggttttgagg tattggtgag tatgggagaa
     4981 caagtgaagg aaggaacacc aatgttgaaa attaaccgtc ctttgattga atcaaagggt
     5041 atatcactta ttactccagt tacaataact aatcattcag agtacaatat gaatacatgt
     5101 aatgtaggca atagtgttga aggtgggaaa gatactgtaa ttgagtttaa ataaagaact
     5161 aaaatacaag gttaatattt ataaatacct ataaacttat gttttataag attgattgta
     5221 tagatttgtt gtatagattt gttgtataaa aaagaactct ttaattataa attaaggagt
     5281 tcttttttat tttgtaaata ttgtttagat ttatatagaa aaacactt
//
LOCUS       DAMJWU010000174         5267 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMJWU010000174 DAMJWU010000000
VERSION     DAMJWU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.179"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(277..477)
                     /locus_tag="PC460_003551"
     CDS             complement(277..477)
                     /locus_tag="PC460_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2602398.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(702..878)
                     /locus_tag="PC460_003552"
     CDS             complement(702..878)
                     /locus_tag="PC460_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602399.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(932..1021)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1201..1401)
                     /locus_tag="PC460_003553"
     CDS             complement(1201..1401)
                     /locus_tag="PC460_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602400.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1403..1753)
                     /locus_tag="PC460_003554"
     CDS             complement(1403..1753)
                     /locus_tag="PC460_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF2602401.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     SKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1915..2104
                     /locus_tag="PC460_003555"
                     /pseudo
     CDS             1915..2104
                     /locus_tag="PC460_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2581..2751)
                     /locus_tag="PC460_003556"
     CDS             complement(2581..2751)
                     /locus_tag="PC460_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602402.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESGYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3140..3634)
                     /locus_tag="PC460_003557"
     CDS             complement(3140..3634)
                     /locus_tag="PC460_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2602403.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKFLND
                     KSNT"
     gene            4439..4816
                     /locus_tag="PC460_003558"
     CDS             4439..4816
                     /locus_tag="PC460_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2602404.1"
                     /translation="MKEEILIEKLLRVELIVMEYLWKKDTLMPKKKIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIINFQSYYKIIIDNKEYYTFKKKVLKSSKSIKIMHSLT
                     TIHKNVSKEKLDLLDEYYRNLKE"
     gene            4921..5267
                     /locus_tag="PC460_003559"
                     /pseudo
     CDS             4921..5267
                     /locus_tag="PC460_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2120 a          633 c          607 g         1907 t
ORIGIN      
        1 gaatacaaaa gaaagagtat gtatttatga gatagttgaa aattttgatg tgccacaact
       61 aaaattatta tattactata tattctaatt aaaagagtac ttgtaggttt aagtacatac
      121 tttataaata tatttttgtc agatttattt tataaaaagt atttagacta agtattattt
      181 ttaagtattt ttttgttgaa gttatttttt ccttttttac aaaatgcgta aataaaaaaa
      241 agactcttaa aagagtcttt ccgaaaaatc tatatattat aatatagtta tattttctgc
      301 ttgaggacct ctagcgcctt taactatatc aaagcttact ttttgacctt cttctaatga
      361 tttaaatcct gaagtttgta tagctgagaa atgagcaaat acgtcatctc cactttccat
      421 tgatataaat ccaaatcctt tttcattatt aaaccatttt actattccat tattcattaa
      481 aaatactcct attaacctat atcaagatat acttgattta cttgtgtatt accacaaata
      541 ctattgtatc atagattaac ataaatgtct atttaattat aatttttagt taacattttt
      601 atttattctt aatatgaaaa taaatttaaa cattttatat taccaatata ttaatgtaaa
      661 tgtagataaa actaaaaagg gttttctaga aaaaaatctg tctaattata cttacttacc
      721 aataccatca cttgatgcaa gcaagattcc acaacttttt ctccgtactc taatataaaa
      781 tcatctaact gtgtatctat aaaatcacaa taatcatatg aactaccaat ataagcagtt
      841 aacatatgtg tacatttatc cattttatct ctaaacataa tgtatttatc cccctaaaaa
      901 actttcatga aaaacaaaaa gaaaatatct ttttggcaac tggctgacat aacttatttt
      961 ataagcgtta gtccctatag ctttgcgtca ctaaatttct ctagttttgc cgatttagtt
     1021 ttattctaca gattaataat acaatagaat tagttattat tcaacatatc tagctgaaaa
     1081 attaataaat ttatatatga acgttaaatt atctgaaaaa ataaaaagcc cctctgtcag
     1141 gagtgactta aaaattgaat ttaagtttgt atttaaaaca catttttaat aatgcaaaaa
     1201 ctaatcaaaa ataaacaatt catcaatagg tgtatctact atttttgata tatctattgc
     1261 taatttcaaa gaaggattat actttcctgc ttctaaacgc ataatggttt ctcttctaac
     1321 tcctactttt tctgctaatt gttcttgagt taattctttt aattgtctat atttttttag
     1381 gttacaagtg aatttaggca tattattttt cctctaaaat ataaagtttt atagcataaa
     1441 catttaaagt gattgcatat gtaattgata gtaaaacaca cattaattct atattcctag
     1501 acactatagt aatacaccat aaaatgaata attctataat agcaagctgt cctgtaaatg
     1561 ccatagctgc tttttcatct tgtacatacc tttcatctgc tttatcacca tttatttttg
     1621 atataataaa gtttgagaaa aaagcaaaaa atccaatata tgtaaggtca gcaacattgc
     1681 cagatgaaaa atatctaaga cttaaaaatc caaggaaacc acaaaaaccc ataaaaaatt
     1741 ttttctgaat cattttttat cctccttagt aattttaaaa gagatatatt tatatcttta
     1801 tgatacaaat atatctcttt tatattcttt tgtcaataga aaatcagtat tatacaatat
     1861 ttgtactgca ccataattgt tagagcaaaa atctactgct tgagatgttt atgtatgact
     1921 aaatatgatt ttaatttcaa attaaaagta gctaaatttt atttgaatag agaaggaaga
     1981 gatattttaa tagtaaaaca atatggagtt tataaccatt ctcaagttga aagatgggtt
     2041 aatatattag gagagtgtgc actaaaaata aaaaaaattt tctatactat atggttcaag
     2101 ttagatgtta taaactatat gatgataact aaatatagtg tacaataaat cgctaagtat
     2161 tttggaataa acaatagcac agttatagca acatgaaaat aaaaattttt acatagtggt
     2221 gttgatgcct tttctaaaat gaaaggaaga ccataatgaa taaaaacaaa aacactaaag
     2281 aaaaaataaa ttaacaagag aacaagaatt agaatatgaa aataaatttt taaaagatga
     2341 gcttgagttt ttaaaaaaga tgtgtgcctc agggaagcca cattaaaaaa cagtggtatc
     2401 attattaatc aaaagaaagt tagatgtatt atcaaaaaat tagaacttaa atgcatagca
     2461 ttttttcata aatcacataa atataactcc tataaatgta taattggaaa agtaaaaaat
     2521 aataaaacaa atagaagatt taaaatatca atagcatatc agaaaataac aaatgatatg
     2581 ctagaattta taaaatatat ctaatgataa aaatggaaaa tcaattataa aaaaggcata
     2641 tcccgattca ttttttgtta tatagtcaaa taagaatact atagatgaaa gatacgattt
     2701 tgcatatgta ttaagtaagt attctgatgt ttttggttga tacacatgca ataaagaagt
     2761 tgtgtttgca gataaattag caaaactata gtataagttg caacaataaa gaaaatgaaa
     2821 attatcatac taaagcataa ggcatgttac aattttatta gataggacat taacatacat
     2881 taaagtactt tttattataa agagtactta tttttttgaa attcattaat gtataaacta
     2941 tcaagaatat tactcaacat aatttaaaaa caacttgaat aggtattttt aaattatgtg
     3001 atataataaa aaatagaagt gtatgccaat acacttctgt aattataact aagcattcta
     3061 ttcttgagga gtgctttttt catttctcca aacaagattg tttatttcat cagcaattat
     3121 taaataaaat ttattgtatt taagtattcg atttatcatt taaaaatttt attacaatat
     3181 ctattttgtt ttgctcgttc attatgtcta ttgaacttcc ataatcactt tttaatttat
     3241 ctagaccact tttgcttatt tctccttctg gaagtatact taattctaga tatttttcat
     3301 gaccaggaga tgacattaat agatatgaac cattataagg catacctaaa ttttctattt
     3361 tatctgctaa tgggtcatct ttaaagccac catttattat tatttttcca tctttcttta
     3421 cccatgatat cattcctcca tcagtatatt ttgattcatc tgcatagtag ctaagagacg
     3481 cactttttat tgcacttata tcagcttcaa gtttagctat ctttgctttt tctatatttt
     3541 taaataatgc tggaagtgct actattgcca atattcctat tatagcaatt actactaata
     3601 attccactag agtaaatcct tttttatttt tcatttattt cccctccata taaaattaat
     3661 aatatataaa tttttatttt gttgatttct actttctata ttttaccata tatagacaaa
     3721 aaaatcactt tctatgacaa atatactatt attttacaat ttatggaaat gatttaaaat
     3781 gacatttctt gttagaaaag atataagaaa tggattaaaa taaagaaaac tacagtcata
     3841 tcaatgtcaa taatattatt taaaagcaga tgaaaataaa aagaggactc agaaaataat
     3901 gtctatttaa ttatacttac ttaccaatac cataacttga tataaacaag attatactac
     3961 atttttttta tgctctagta taaaatcatc taactgtgta tctataaaat caaaataatt
     4021 atatgaacta tcagtataat aagtcaaaat atgtatacat gaatacatat tttaaaagat
     4081 atatattatt acatacctaa aaaactttca taaaaacaaa aataaaatat gcttttagta
     4141 acttgttaac ataacttata atttatttta taggcattag cctatatagc tttgtgctac
     4201 taaatttctt tgattctagc gatttagtgc tattctacaa ctaaaataat acaatagagc
     4261 tatttattgt ttggtagcat tgtttgcaaa ttaaacaaat ttatatatat gaatattatt
     4321 ttacgtttaa aaaaacagac taaatatgta gaatatttgg taaaacttac ctctagtttt
     4381 tgtcttaaaa agctcctatt attaatttaa gttagaagaa ttaaggaagt gacataaatt
     4441 gaaagaagaa atattaatag aaaaattatt aagagtagaa ttaatagtaa tggagtattt
     4501 atggaaaaaa gacactctaa tgcctaaaaa aaaaattgta aaagaaataa aacagattta
     4561 tggatggcat aaaagtacaa taaaaatact actaaaaagg ctagttgata aaaggtattt
     4621 ggctagatat attataaatt ttcaatctta ttataaaata ataatagata ataaagaata
     4681 ttatactttt aagaaaaagg tactaaaatc tagtaaaagc ataaaaataa tgcattcact
     4741 taccactata cataaaaatg taagtaaaga gaagttagac ctattagatg agtattatag
     4801 aaacttaaaa gaatgatata gtaaaaatcg aatgtaatta tttaccttaa ttcctttgtt
     4861 tgtaaaactt tattgatata gaatttgaca tatacataaa ttggtaaagg gatgtatact
     4921 atgaaaatta gtaaattacc agaagcggaa ttaaaagtta tgagatatat ttgggaatca
     4981 gaaaaggtat taataccaag agaaatcacc aagtctatgg aacaaaaata tgaatggaaa
     5041 gatacaacta tatttacagt attaaaaaga ttactaaaga agagagtttt tagaagcgga
     5101 gaaaatagat aagtgtacac actataatgt attagtaaaa aaaaataata tcagagattt
     5161 taaacaaaag aatttttaaa gaatacacat aaaaattcaa tattaagttt aatgtcagca
     5221 ctatgtagta aggatgatga agtagatggt gatattttaa atttatt
//
LOCUS       DAMJWU010000175         5188 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMJWU010000175 DAMJWU010000000
VERSION     DAMJWU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.180"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            61..1833
                     /locus_tag="PC460_003560"
     CDS             61..1833
                     /locus_tag="PC460_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602405.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            1855..3276
                     /gene="ccpM"
                     /locus_tag="PC460_003561"
     CDS             1855..3276
                     /gene="ccpM"
                     /locus_tag="PC460_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HDF2602406.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3305..4375
                     /locus_tag="PC460_003562"
     CDS             3305..4375
                     /locus_tag="PC460_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HDF2602407.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4391..4642
                     /locus_tag="PC460_003563"
     CDS             4391..4642
                     /locus_tag="PC460_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HDF2602408.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4693..4896
                     /locus_tag="PC460_003564"
     CDS             4693..4896
                     /locus_tag="PC460_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602409.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2113 a          455 c          830 g         1790 t
ORIGIN      
        1 ttatattata ttaattaata aaatatttta tttcaatttt attaagtatg gaggtgtatt
       61 gtggaaaatg gtaaatttca attttttaaa ggtgtaagaa attgttttaa tatgatttat
      121 ctacatgata aaagatattt atttatctct atgtttatgg caatttttca ggggattgtt
      181 ccatctattt ctttgttagt aagtcaaaat atcattaata atatgcaaac attttcgaaa
      241 agtgtaaaag aaatattagt tttgttaatt atgtatttaa tcataaatat aattccttca
      301 atttttcaaa gtttttatgg atattataga ataaagttta ataaaaattt tgaaaaacat
      361 attgaaacaa tgatgttaga gaaatcttta actttgagtt taaaagattt tgaggatgat
      421 aatacttatg acatgataaa tagagctcaa tctcaaaaag gatttaatat tactatacta
      481 tctgaaaacc ttatttcaat aataagagaa tcagtatcta cactttctct tattgttgta
      541 ttatcacaat ttcaactttg gctaataata gttgttattg ttattcctgt attgaaatca
      601 ttatattcca ttaaattagg aaaggaacaa tatgatgtta gtgtaaacag aacttggaaa
      661 gaaagaaagg cctggtacat agattatttg ttgtcaaaag gttatgctta caaagagatt
      721 atggtattta gaattggttc taaacttatt aatcattata aaaaattaaa agaggatatt
      781 atatctcaag atatgaatat agctaaaaag aattttattg ctacgctatt atgtacagtt
      841 ttagattcaa ttgtatctgc aggaattata ggatttataa taattcaagg tattttaaag
      901 aagattttaa taggagatgc gactacatat atacaagcag ttcaatctat aaaggataat
      961 gttgaatcaa tattttcgtt atttagttca gtaatacaag atctttttta tacagattta
     1021 ctgtttaaat ttctagatta taactgtaaa gaagactcaa ttgaacatat aaaaatattt
     1081 gaaaaaatag gggatataaa taaaatagag ttaataaata tttcatataa atacaatcat
     1141 tcatcagagt attctttaaa aaatattaat ttaactttaa ataaaaatga tcccattgct
     1201 atactaggaa aaaatggttc aggtaaaacg acacttttaa aaatattatt aggtttttat
     1261 gatgattatg aaggtgacat cttgataaat ggaaaaaatt taaaggatat tgaaaaagat
     1321 gaatacatta gaaaagtaag ttgtatgttt caagattata ttaaatatga ggctacatta
     1381 aaagaaaatg tcgccatggg agatgtatat aagagtaact tggaagaaaa agttaaaagt
     1441 aaacttaaag aaactaaatt gaaaccaagt atttatgagg aagatggatt agaaactatt
     1501 ttaggaacct ggtttggtaa gaagcaactt tctatgggtg aatggcaaaa agtagcaata
     1561 tctagaactc ttatgaaaga tgctgatatg tacatttttg atgaaccaga ttcatcttta
     1621 gattctgaag cgggaaatga actcctttct ttatataaaa aaacatttga aaataagatt
     1681 ggaatattta taacgcacca tatccaacat gcaaaaaaga taactaacaa tattgtagtt
     1741 cttgaaaatg gaaagttgtt agaaagtgga agacatgatg aacttatgga caaaaagggt
     1801 gtttatttta atttattttg taactgcaaa taaatattaa agggaggtta attaatgaat
     1861 caaaaaccat ttatacacac atttaaagct ggaaaatcat attttattta tgatgttaat
     1921 acggataaga ttttaaaggt aaatgctgct gtttataatt atctaaataa aattgagcat
     1981 aatttagaaa aagaaagtga ctggaacata gaaatagaag acgaaattaa taccttgatt
     2041 aattatggat ttctaaaaaa taaaagggta agtaaaacaa tgcaccctga aactaagtat
     2101 ataaaatatc atatagaaaa taatctatcc tctattattt tacaagtaac ccaaaattgt
     2161 aatttacgtt gtgactattg catttattct ggttcataca gcaacagagt tcatacaaat
     2221 aaaagaatga atatagatgt agcaaaaaga agtattgact atttgattaa gaattctagt
     2281 caattaaatg aagttagtgt tggcttttat ggaggagaac ccttacttga gtttgatttg
     2341 attaagcaga tagtagaata tgtaaaagta gttggagaag gaaaggaaat atactttaat
     2401 ctaactacaa atgcaacttt atttaatgag gaaataatag aattctttgt aaaaaataat
     2461 ataacaacta tgattagttt agatggagga aaagaaattc atgataaaag tagaaaattt
     2521 gcaaatacag gagaaggatc tcatgatgtt gtattaaaaa atgtacgtta cataaaaaat
     2581 aaatatccag aatattataa aaagaatatt acatttaata cagtgataaa ttcagaaaat
     2641 gattacattt ctatagatga ttttatatct aaaaatgaga ctttaaaaga ttcaaacttt
     2701 ttagcttcca ctcttaataa taactattct gataaagaaa tagtttcatc agatgaatat
     2761 atattaggta agagttatga tagattcttg actttactca acaaattaaa atttataaaa
     2821 gatgagagta tgtcaccttt atcaagaata gaaagagcct tattagatga ttttagatat
     2881 gagaaaaata ttgaaggtag attagagtta ccagatgtgt ggcatcatgg tggaccatgt
     2941 ataccaggag cttttagaat atttgtaact acagatggag aattttttcc atgtgagaga
     3001 gtaagtgaaa gttctaaaat gggtatgatt ggaaatttag atagtggagt agatatagaa
     3061 aaggctgaaa aacttttaaa tataggaaaa ttatatgaag agcaatgttc tgattgttgg
     3121 gcctatagtt attgtgatat atgtatagct aagattgatg atactaaatg taatgaaaat
     3181 gaaataaaac cacatgattg tgacagaatt agacattcta cagaaaataa tatgaaagat
     3241 tactgcgttt tgaaatctct aggatatcaa tattaaattt tctaatgaaa ggaaagatgg
     3301 gattatggaa aaagtactta tatatccatt taattataat ttttttacta ttttaaatca
     3361 ttgcaataaa atttcagata tagaaattaa ttcactggtt agtccttctg gctgggggtt
     3421 atgcaataag gagtataatg ttaataatat tattactaaa gttacaaatg attttcaaag
     3481 tgaacttaaa aagaattctt tggtatggtt tgttgattca aaagaaaatc tagacttaga
     3541 aaaatttata attcctaaat taaaagtagc tctaaaagaa ggtaaaaaga tattatatac
     3601 acgtgatgat cttaataaaa tatctaggta tatatctcat tatgaattca aaaagattaa
     3661 tgaatggaat ttagaaaaat atgaaataag agatatagag ggtatatcac agataaacac
     3721 accaataata tttatttgtg gtctttatgg agggttagat acttttgatg taaatataag
     3781 gataggagaa gaactaagaa aaagggggtt ttcaatatta caatttggcc ctaaaaaaga
     3841 gagctattta tttaaaatga ttcctatacc agagtttatg tttgatgatt cgataagtag
     3901 taagacaaag attattaatt ttaaccattt tataaaagat aaagaggtag aggcaaagcc
     3961 agatttaata attatagaag ttccaggaga attattccct ttctctagaa aaatggttgg
     4021 taattttgga attagagctt ttgaaatttc taatgcagta ataaatgatt gtggtatatt
     4081 atgtattcca tcaagtatat atgatagaga atattgtgaa gaacttaaat attttattaa
     4141 aggaagacat ggtataaata tagactattt tagtatagag aaaaaagtta tagatacata
     4201 tgagacagag gtgaagcaaa aattttcata tatgtcatta gatgaaaact tttttgttga
     4261 agatatagaa gattatgaaa aaacattttt tatacatgat aataaacaat tactagaatt
     4321 agtggacaat atagtgaatc aattaaaaga gtatgctaaa gtaatatcta tttagaattg
     4381 gaggttaatt atgaattaca ctgaaaagga attactacta gagaatatat tttttgatgt
     4441 gactggaatt aattttaata aaaataacaa tttaaaaaaa gaattatttt tttcaccgtc
     4501 tttacatata cagccaagag aattactttt agtattttgc gaggtaatag aaaactttaa
     4561 tataaaaatt ccagaggaaa aacttttgaa taggcaattt acgagttttg ataatgttct
     4621 aaaaataatc aatatggtat aggttaataa aggtataaag taagtgatct tatagaaaag
     4681 gggaggatat gaatgagttt tggaaaaaga atagaaatga ataatatagg tgttgagaca
     4741 agaggtgttt gtactattat gtgtccttat acttgtaaga tgatggcatc ttcatcttgt
     4801 aactgcaaga ataatgatta taatgcaaga ggaggatcat ataatacata ttataataaa
     4861 ttgatacatg gccaaagaac tcctgatgga atgtaaatat ttcagtaata agtatactat
     4921 taaattgatt tagcatattt gattaaattt atctttctta ctttatttcc ttttattata
     4981 aaaatatgca gttgattata attaagagtg tgattttata ataactgcat aaatagtatc
     5041 tgttatataa atcacctctt caattattat agtggtaaat atagtgatta ggggtgattt
     5101 ttacaattta aaagtataaa ttaattaaaa gttattataa taaattatac ttatttaaat
     5161 agtaagaatt aaaagtaaag tttttaaa
//
LOCUS       DAMJWU010000176         5088 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMJWU010000176 DAMJWU010000000
VERSION     DAMJWU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.181"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            74..1957
                     /locus_tag="PC460_003565"
     CDS             74..1957
                     /locus_tag="PC460_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF2602410.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2182..2862
                     /gene="pgmB"
                     /locus_tag="PC460_003566"
     CDS             2182..2862
                     /gene="pgmB"
                     /locus_tag="PC460_003566"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2602411.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2859..>5088
                     /locus_tag="PC460_003567"
     CDS             2859..>5088
                     /locus_tag="PC460_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2602412.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIK"
BASE COUNT         2102 a          458 c          962 g         1566 t
ORIGIN      
        1 tgtgatataa tagtaaaata tattaaccta tctttagatt atataaatta ttataaagga
       61 gggaaatttt aaaatgtcta ataaaaataa agaaaatata actttaagta atcaaatcaa
      121 aggaacaatt caaaatagtt taacaaaaga agctatatta catataccaa tgagcaatta
      181 tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa aaaatgaagc
      241 gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggag gtgcaggagg
      301 aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatatag taatgagtaa
      361 agaagtagaa actgaattat ttgactattg gatagctaag tgtaagcctt taaataaacg
      421 ctcacgatat ggctttataa tagaaggtca agaggaaaaa cttttattta cagaaaaaag
      481 aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag gtaatttttt
      541 tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg tgaaagatac
      601 tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta taaacccaga
      661 aggagtagat aaatggggag ctattccaac cagagataat tttacaggtg gagatttaca
      721 aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa tttacttttg
      781 ccctattaca attggaaaaa ccaatcatag atatgatacg gtagattata tggaggtaga
      841 ccctacatta ggagataaag agactttaaa aaaactaata gaagaagcgc ataagagaaa
      901 tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta aacagtggca
      961 agatgtagtt caaaataagg aaaattctcg atataaaaac tggttttata taaaagatat
     1021 gagtaaaatt gatactccaa tagaacaaat agatgaaaaa aatatacctt atgaaacctt
     1081 tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa taaaatattt
     1141 attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga gattagatgt
     1201 ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca aaaaagtaaa
     1261 accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggc taatgggtga
     1321 ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat tcttttgtac
     1381 agatgagata aacgccgaag aatttaagta tatgataaat gatgtaatgg taagttatcc
     1441 aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta caagaatatt
     1501 gacttatgct aatggaaata ttgatagatt taaacttgca tatatgttta tgtttgtaca
     1561 aacaggatgt ccttgtatat actatggtga tgaaatcgga atggaaggag aattaagttc
     1621 aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga ataaggatat
     1681 atttgatttt atgaagaaga ttattagact tcgtaaagaa aataaagaac ttaggtctat
     1741 atcaaatgaa tgggtgttag cagataagga aaatggaacg attatattga aaaaagaatc
     1801 tatttctata ataataaata attcatctaa aaatataatt ttgacgttac cagattattt
     1861 agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa aagaagaact
     1921 aatgttagag aaatacaagt tcattatttt aaaataatac aaaaaagtat ttattttaga
     1981 tttcatgtta aattattata cagtttaaat aagtctttat aaacgataaa aaaatatggt
     2041 ctacaagtga gtgaattgaa agtagatttt aaattaaaga ctgagttaga gtagataatt
     2101 ttataaataa tatcttaaat acaagataga aaataaattg attattagtc taaaaatttc
     2161 ttgtagaaag aggaaataag aatgattgaa gcatttatat ttgatttaga tggtgtaatt
     2221 acagatactg catattatca ctatatggct tggagaaaac tagcccataa agttgggata
     2281 gatatagaca caaaatttaa tgaatcttta aaaggcataa gtagaatgga gtcattggac
     2341 aggatattag aatttggaaa taagaaatat tcattttcag aagaagaaaa agtgagaatg
     2401 gctgaagaaa aaaataatta ctatgtatca ttgatagatg agataacttc aaatgatatt
     2461 ttgccaggta tagaaagctt gttaattgat gtaaaatcta ataatataaa gataggtcta
     2521 tcttctgcaa gtaagaatgc aataaatgta ttaaatcatt tgggtataag tgacaaattt
     2581 gattttatag ctgatgcagg aaaatgtaaa aacaataaac cacatccaga gatatttctt
     2641 atgtcagcga aagggcttaa tgtaaatcca caaaattgta tagggataga ggatgcaagt
     2701 gcaggtatag acgctataaa ctcagctaat atgttttctg ttggtgttgg aaattatgaa
     2761 aacttaaaaa aagcaaattt agttgtagat tcaacaaatc aattaaagtt tgaatatata
     2821 caagaaaagt ataatgagta tatagttagg agaattatat gaaatgttta tttagtaatg
     2881 tagatgagtg gaagattata caagataaga ttgaaatcaa agaaaataga ttagcagagt
     2941 ccataatgag tataggcaat ggatatatgg gtatgagagg aaactatgag gaaaattata
     3001 gtggagatac acacaagggg tcttatatag ctggagtatg gtttccagat aaaactcgtg
     3061 ttggttggtg gaaaaatggg tatccagaat actttggtaa aattcctaat tcagtcaact
     3121 atataggtgt aagaattttc atagatgatg tagaattaga tttagcaaaa tgtaaagtag
     3181 aagattttta tagagaatta gatatgaaaa atggtatttt aaatcgtagg tttattgtaa
     3241 atataaatga taaaaaattt gaagttaggg ttgctaggtt tttaagtata tctgttaagg
     3301 aacttgcagt aattaaatac agtataaaat cattaaattc taatactaaa ataaaatttg
     3361 taccatattt agattcaaat ataaaaaatg aagattcaaa ctatgaggaa aagttttgga
     3421 atggaatttc ttcaagttct ttagaatata aaggtagtat tctttctcga acaaaggaga
     3481 atgcatttaa tacaccagta ttcactgtgg gttcaatgat gtatattaag acaaatggtg
     3541 atgtagaagc tgtatctcat gagagtgatt ataattactg tgaaaattcg ataacaatgg
     3601 atgttttaaa aaatgaggtt gcaagtatag aaaaatatgt atctataact tcaactagag
     3661 attataagga aagtgagcta ttaagaaaat gtgaagacat tttaaataga gaactatcta
     3721 aaagttatga tgatatatta aatgaacata caaagttatg gaataaaaga tgggaaagtg
     3781 cagatgtaaa aataaatgga gataaaagta gccaacaagg tataagatat aatttattcc
     3841 aactattttc aacatattat ggagaagatt cgcgattaaa tataggtcca aaaggattta
     3901 ctggtgaaaa gtatggagga gctacttatt gggatacaga agcgttttgt gttccagtat
     3961 atctaggtgt agcagataag gaggtaacta gaaatctttt agaatatagg tacaatcagt
     4021 tagaacaggc aaaggataat gcaaaaaaac tagggcttaa aggtgcttta tatccaatgg
     4081 taacatttga tggagtagaa tgtcataatg aatgggagat aacatttgag gaaatccacc
     4141 gaaatggaag tatagtatat gcaatatata actatacaaa ttatacaggt gactataaat
     4201 atataaaaga aaaaggaata gatgtaataa tagaaatagc tagattttgg gcaagtagag
     4261 ttcatttgtc tacaaaaaaa gatttgtata tgatacatgg agtaacaggt ccaaacgagt
     4321 atgaaaacaa tatcaataat aattggtata ctaattatat agctaaatgg tgtttagatt
     4381 atgctgtaga aaatatctta cgattagaga aagagtgtaa tgaatgtatt gacagaaata
     4441 atgtgactag ggaagaagtt gagttttgga aaataatatc aaataaaatg tatctgccgt
     4501 atgatgagga actacaaata ataattcaaa atgataattt tttagataaa gaatttgtag
     4561 aagtaaagga tttaccaaaa gaaaattttc ctcttaatca aaattggtct tgggataaga
     4621 ttcttaggtc gtgttttata aaacaagcag atgtattaca gggaatttac tattttggaa
     4681 atagatttac taaagaagaa aaaaagagaa attttgactt ctatgaaaaa tatactgtac
     4741 atgaatcttc tttatcttct agtatttatt ctattattgc ctcagaaata gataacctag
     4801 aaaaggctta tgaattatat tcaagaacag ctaggcttga tttagataat tacaataatg
     4861 atacaaatga tggactgcac ataacttcaa tgtctggttc atggctttcc attgtacatg
     4921 gatttgcagg catgagaaca tggaatgaaa ctctatcttt tgaccctaag ttgccaaaag
     4981 agtggaaaaa ttattctttc aatataaatt atagagataa tattattaat gtagatgtat
     5041 gtagtgattt aattaaaata gaaaatttaa aaggtagacc aataaaaa
//
LOCUS       DAMJWU010000177         5087 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMJWU010000177 DAMJWU010000000
VERSION     DAMJWU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.182"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(143..628)
                     /locus_tag="PC460_003568"
     CDS             complement(143..628)
                     /locus_tag="PC460_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2602413.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(711..804)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1158..1541)
                     /locus_tag="PC460_003569"
                     /pseudo
     CDS             complement(1158..1541)
                     /locus_tag="PC460_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      1767..1864
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1919..2095
                     /locus_tag="PC460_003570"
     CDS             1919..2095
                     /locus_tag="PC460_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602414.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            2321..2521
                     /locus_tag="PC460_003571"
     CDS             2321..2521
                     /locus_tag="PC460_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2602415.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(2685..2897)
                     /locus_tag="PC460_003572"
     CDS             complement(2685..2897)
                     /locus_tag="PC460_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602416.1"
                     /translation="MVCKIFITLKTILPKTQIIVTTHSPHILQIDSKEEMIVLDMAES
                     DNVYKKELKLGEYGVLGWTNEGLYLH"
     gene            3272..3454
                     /locus_tag="PC460_003573"
     CDS             3272..3454
                     /locus_tag="PC460_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2602417.1"
                     /translation="MFKFYNNATLMFLPGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            3525..4370
                     /locus_tag="PC460_003574"
     CDS             3525..4370
                     /locus_tag="PC460_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602418.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNKDPKYNLNTI
                     KKHLN"
     gene            complement(4594..4758)
                     /locus_tag="PC460_003575"
     CDS             complement(4594..4758)
                     /locus_tag="PC460_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602419.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1890 a          657 c          547 g         1993 t
ORIGIN      
        1 catttataat tactttataa taaaaataaa ttagttaatt tattctcttt agttctgtta
       61 taatgtttct acaaaaaaga atcccattaa tgggattctt ttttgcgatt tattaatata
      121 tttaaagctt gctagtatcg tattataaac ttaaatgctc ttctatgtat tgattatata
      181 catatccaga atatcgtttt tcatttttat tgatattaac tcttgcccat tttccaacgc
      241 tataacatac ttcaacttta aaaccatcag ggattgtagt tataacttta gattctaatg
      301 ttctatcatt tctaagttct aaacttaatc catttgttct tatatttcct atgtaattct
      361 tttttctatt ttttattgta tcattactat tgtttttagc attacttaat ctttctaaat
      421 atttataaag taaccaacct ttgtcataat tactctcaac ttctgcccat attccatcta
      481 cacactttaa cttaacaatt ttaccttctg gtatactttt taatttcaaa ctatttatgt
      541 ctggtttttc tcttaaattc aatttaattt tagtcttaaa attgatgcta gatttgcgaa
      601 aattttttaa cattaattca gatatcatct taagccctcc tcttgccaaa tattaagtta
      661 ttaaaatata taactctaaa aatactttta acaaaaagaa ttattatctt tttggcaact
      721 ggctgacatg acctttatta cataaagatt ttagtcccta tagttttgcg tcattagatt
      781 tatctaatat tgcctatatc tgaataataa cataatttga acaattaaaa aataatatta
      841 cctcaaattt tatattattt aaatataaat taaattttta attaataata atcaaaaaat
      901 atctttatat ttattcaatt actgttcttt atagtcaata taaaagacag taatttatct
      961 atagtgttac ttatatttta tatatatggc tatttttcaa cattttacaa tatataacat
     1021 ttattttttt aacttttgtc agtaaaatgt cagcaaaaca ctttatccac aatttttata
     1081 taagttttcc acaacttatc accaatatta tccacataaa aaaggtagca cttatattat
     1141 tagctacctt ttatttatta atctttatat ttccagcttt ttatccaact tcctatttct
     1201 attatttttt cttcagttat ttcttctcca tcatgtaatt ttgaaattaa ttctgataaa
     1261 ggattgttat gtaaacctct aaaaactctt tttccctcta ttttgagata ttctctctct
     1321 tttattaata ctgtatagtg tacatgcctc ccaattctct atgatattaa aaaacctttt
     1381 ttggttaatc tagctaaaag tataattgta gttgtatgct tccaaccata tgttttttct
     1441 atttcatctg caacatcttt tgatattaca gttttataac cagtatgcca tatgcatttc
     1501 ataattttta attctgtatc tggtaatttt ttatttagca taatatattc cttctttcaa
     1561 tttatgtcac ttcttcaaat ttatctagct taaattataa taggagcttt ttagaacaaa
     1621 aactatattt aatttttacc aggtactcta cgtgtttagt ctgtttttaa gtgtaaaata
     1681 atgttcatat ctataaattt atttaacttt caaacaatcc tgttgaataa taactaattc
     1741 tattgtatta ttttagttgt agaataaaac taaatcggca aaactagaga aatttagtga
     1801 cgcaaagcta tagggactaa gacttatata aatatcttat gagttatgtc agccagttgc
     1861 caaagagata gtgttctttt tgtttttatg aaagtttttt agggggataa taatttatat
     1921 gtttggaaat aaaatggata aatgtacaca tgtattgact gcttatatta gcagttcgta
     1981 tgattattgt aattttttag atacacagtt agatgatttt atattagagt acggagaaaa
     2041 tgtagtagag tcttgtttac accaagtaat ggtattggta agtaagtata attagataga
     2101 cattgatttc tagaaaaccc tttttagttt tatctacatt tatattaata tatagcaatg
     2161 taaaacgttt aaatatattt ttaatattaa gaataattaa aatgttaact aaaaaatata
     2221 attaagtaga catttctatt aatctatgat acaatagtat ttgtggtaat acacaattaa
     2281 atcaagtata tcttgatata ggttaatagg agtattttta atgaataatg gaatagtgaa
     2341 atggtttaat aatgaaaaag gattcggttt tatatcaatg gaaggtagag atgatgtgtt
     2401 tgctcatttc tcagctatac aaacttcagg atttaaatca ttagaagaag gtcaacaagt
     2461 aagctttgat atagttaaag gtgctagagg tcctcaagca gaaaatataa ctatattata
     2521 atatatagat ttttcgggaa agactctttt tagagtcttt ttttattgtt aatatatgta
     2581 agataatcat tggtgaagta aataaggtat attgagtaat gttcttgata gtttatatgc
     2641 taatgaattt caaaaaaata agcactccta taaaaagagt gctcttaatg taaatataaa
     2701 ccttcattgg tccagcctaa taccccgtat tctccaagtt ttaactcttt tttatataca
     2761 ttatcacttt cagccatatc taacacaatc atttcttctt tagaatcaat ttgtaaaata
     2821 tgaggactgt gtgttgtgac aattatttgt gtttttggga gaattgtttt taatgtaata
     2881 aaaattttac ataccacata gttgatataa aacttaagac tagcagactc ataagcaatt
     2941 agttgcctct ctttacatac cacatagttg atataaaacc tagaggctct cagagaagtt
     3001 ataagatgtt tagtaacttt acataccaca tagttgatat aaaactccaa aataaactta
     3061 gcatctccaa tgtttacaca tatacaatgc tcttaaaatt gcagtgaacg cgcaatattg
     3121 caattgataa tacttatcat atattctcaa taccttatat ttcaactgct aaacctcaat
     3181 ttatcacaaa tatcgctcac tgcaaaattt ctatattttt attatatcat agcatataat
     3241 taatattttc aattaatttt tacatttaat tatgtttaaa ttctataata atgcaacttt
     3301 aatgtttctt cctggaacta atataaaaac tatttcagaa agattaggtc atgcagatat
     3361 aaaaataact atgaatagct actctcagtt tttagaagag atggacagag aagcatttga
     3421 aaatttgagc aaaatactat ttattgtatt ttaaagaaga tgacttattc aatatttgat
     3481 gctagaatat aattaataaa aatttaatta taggtgattg tattatgaat attattaaaa
     3541 gcataacttt actcttaaca ttattaattg tgatattaat gacaggctgt tccccaagag
     3601 aaagtgctag tgaagttgca aaaaaatata ttgataagat gaactctggt gactatgaaa
     3661 cagcatatag tttattaagt aaagatagta aaaaaaaaat tagtatagaa aatcttgaag
     3721 aatttcaaaa tattttagtt agtacaaaaa gaataattag ttataaaata ggtaaagaaa
     3781 aaatcataaa aaaatataaa catgatggaa aagaatataa aaatgttgtt aaattagaag
     3841 aaacttttaa tataaaaaac cttctttata aaaaagatga ttctctaaaa gtaaatagat
     3901 attttgtagt tgaaaataac aaatacaagc tattattgta tgaaagtttc aaaaaagaat
     3961 ttggtattaa tagtatggac cttgctcaac ttaagcttaa tgattttgga ctaaaatact
     4021 ttaaagaagt tgaccttata ctaaaaaaag ctattgctat taatcctact gattcaaagc
     4081 tttattatgc ccaagcatgt aattatatga attttgatgg atttcctggt tattcagata
     4141 caacatataa tgctttagat tcaattaata aatctttaaa gtatttagat aaaaatgatt
     4201 ctgactacaa aaaaaatgca agtaatatat ataacttaaa aggagttatt cttatgtttc
     4261 acaaaagatt tgatgaagca caacaatatt taaataaagc cttatacttt aacaaagata
     4321 ataaagaccc aaaatataat ctaaatacaa taaaaaaaca tctaaactag atatttaatt
     4381 tttgaattta taaaatattt ttttatgtat aattcagcac tgaatcataa tctctactaa
     4441 actaattaca agtttaaatt ataaagactt atttctatat aaatattact ctataacaat
     4501 aagtttctta tttaaatttt aaaatattat ctaaaaatca taaaaaagaa ggtataaaga
     4561 gctaccctca agctcaatat accttataaa tatctaaact tcatttttta ctaatttttc
     4621 tatatctttg ataatatgtt ctttagattt ttttccagct atggcatcaa aaattgcaac
     4681 agcctttttc tctccaaact tttctgctac atcattaaag tctaaaaatc taacactttc
     4741 ttttggttta ccaaacatat aacacctctc atacattttt atatatttat tataacttct
     4801 atagacttat ttttcaccaa taaacgttag acaaattagt ccctttattt ttttaaacta
     4861 gctttacctt caaaattctt aacaaaaaca gtttattcta ctctataaat aaaaatagac
     4921 aatttaattt gtaggaaact gataatagaa tacagtaaat aaaactttgt ttaatctatc
     4981 tgaaaaagtt atctattaaa actgctatga atttataaag aatattctag ttcataaaaa
     5041 tacaaatatg taaatataat caccttaata ttcaatttat ttaaaat
//
LOCUS       DAMJWU010000178         5080 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMJWU010000178 DAMJWU010000000
VERSION     DAMJWU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.183"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            340..3174
                     /locus_tag="PC460_003576"
     CDS             340..3174
                     /locus_tag="PC460_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2602420.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            3217..4461
                     /locus_tag="PC460_003577"
     CDS             3217..4461
                     /locus_tag="PC460_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2602421.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVVKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT         1986 a          659 c          733 g         1702 t
ORIGIN      
        1 attattctta accttatcag ttttttcata tatcttcatc tataccgacc atatattaat
       61 tacattgacc atatattaat aaatgatatt acatagactt aagtatatta aaaaacttaa
      121 atttagttat ttaacaacat aatactctaa tttaatctat aaaaatccat acctaatata
      181 atgcaactct tattttaaca ccatctatta cacaaaacaa catattaaaa agtaaaatac
      241 aaaatatcta ctataaacct tattttaata ctgaatatat attataatat atttaatgta
      301 gcttaaatat agtttaaaca taaagaaagg aaacggtata tgctaagaaa agataaagtt
      361 ctattatctc tacataaatt atgtgaaaat atgactttaa aagaccttca atcagataaa
      421 acaggattca ctacaataga aattgctaac cattcatctc ttgatagaag taataccagt
      481 aaagaattaa ataacctata caatgaaaaa aaagttctga aaatatctgg taagccaatt
      541 ttatttctac atgctagtat tatagaaaat ttaattggcc gtactttgaa cagtgatgaa
      601 ttttctataa ataattgtaa tgagctactt aaatcaagta aaaatgactc agaagatatt
      661 ttttcaacat tacttggaca tgattctagt cttaaactac ctatagaaaa agctaaatct
      721 gctatactct atcctccaaa aggtcttaat acattactaa taggacctac tggtgttgga
      781 aaaagtacat ttgctgaaac aatgtacaaa tatgctgtag aaagtaaaat cttttcttct
      841 gaaagcaaat tcgtagtatt taactgtgct gaatatgcag aaaatgctaa cttattatta
      901 agtaatttat ttggttatgt caaaggttct tttactggtg ctaataagga taaattcggc
      961 atagttgcac aggctgatgg aggcatatta ttcttagatg aaatacacag attaccacca
     1021 gaaggtcaag agatgctttt tcttttgatg gacaaaggca tatacagacg attaggagaa
     1081 tctgacattg tgcataaagc taatgtcttt attgtgtgtg ccacaacaga agatatacat
     1141 tcttctctgc ttggaacttt tttaagaaga atccctgtta caatcaacct acctgcttta
     1201 caggataggt ctttaaaaga aagattatct cttattaaat acttctttat tatagaatca
     1261 aaatacacca atgcaactat aaaagttcac aaagatgtaa ttaaatcatt actcctttat
     1321 gattgttttg gtaatatagg tcagcttaaa tctgacatac aacttatgag tgcaagagcc
     1381 ttcttaaatt acaaaactgg acttaaggat aatatagaaa tagatttatc ccttgttcct
     1441 gattacatct atattggtct tttaaaattg aatgaaaatc gtagtaatct aaataaatta
     1501 tcagaacttg attatatgct tttttatgaa tttaatggta ataacttaga agttgaatat
     1561 acaaaagaac tatctaaata caatatagat acatcaaaca atggatttaa atactctaca
     1621 gagattcctt atctggaaaa tgatataagg agtatgttta taaattacat ggaaaagtac
     1681 tctacaaaat tactttcgat atcttcagta actcattctg attatacaag taatgagatt
     1741 tttaaagtaa tcaatccaaa gatttactat gctgttgaac attctttaaa agaggctgaa
     1801 aaaatatcga gaaacaaata cacaaaacaa acttatattg ctctttcaat gcatataagt
     1861 gcacttatag aaaatataaa tcaagacaca tataatccta agtacaattt atataatgat
     1921 aaaattttct tagataaaga tttaaatata gctaaaatag ttttaagtat aattgaaaat
     1981 gacttacaaa catctttacc taaagatgaa gtaaaattta tatccatgtt tctaaatagc
     2041 atgtatcttg aaccaaacaa tcaatctgaa agtataggag tcataatact tgctcatgga
     2101 aattcaacag caagcagtat gtgtgatgta gcaaattcat tgcttggtac aaaccattgt
     2161 aatgcaattg acatgcctct tgatgtaaaa gtaagtacca tacttgataa aactatagag
     2221 ttggtaaaag aatgtgacga aggtaagggt gtacttcttt taacagatat gggctctctt
     2281 atagcttttg gagaacttat ttcagaaaaa acaaaaatca aaactcgctc aattgaaatg
     2341 gtatctactc caatagtttt agaagcagta agaaaaagta cactaccaga aatgactttg
     2401 gatgaattag ctaattcctt aagctcttta aatccatata ttggacgagc agcaatcgat
     2461 tcaacagaaa taaactatcc tgaaaagtca agagagtatg taataataac tacttgtata
     2521 acaggatatg gttcagctat gaaagtagca gactttataa attcttcact taaaaatatt
     2581 agtaaataca atattaattt agttcctcat aatatagaaa gttttaaaat gtataagaat
     2641 aaatttagta atactaaaat tctggcagtt gttggaagtg tagattttaa tgtagctaac
     2701 acccctttta tacacataga ggaactacta aatggatatg gcctagatat gctaaacaaa
     2761 ataatagaag gtacattcaa tgccaatagt attacaaata taaaagaaac aaatctatca
     2821 tacaacagtt taataataaa ctttatgacc caaaacttag agcttttgga ctctgttaaa
     2881 ttattcaact atgtcaattc ttccttattt acaataatag aattaataga tgaaaactta
     2941 aagagcaaat ttaagctagg atatatacta cattgctctt ttatgataga aagatgtttt
     3001 aaaaatacaa cttttccata taataacatt actgaattaa ttagtaaaaa tcatgaacta
     3061 tatagtttaa tacgtgaagc ctttaagcca acagaagctt attttaatat tttgatacca
     3121 gacgaagaaa ttgcttatat aatagacatg attaatgagt atctaaataa ataaaattta
     3181 tagcacttat tttgtgcata ttaattaata tatgaaatgg aggacgagat tttgaaagga
     3241 aaaataaaac agttaacaat acttgcatta atttttatat ttataactcc tgtatttgca
     3301 tttgcagata ctccaccagt accaaattct tcaagagcag cactacttat agaccaggaa
     3361 actaaaagaa tattatttga aaaaaacatt gatgaaaaaa tgccattagc gagtttaagt
     3421 aaaatgatga cctttttatt ggcaatagaa gcagttgata aaaatcaagt taaagagact
     3481 gatatggtga aaatagacaa gtctacagct tcagtaggag gttctacttg taaacttaaa
     3541 gatggtgatg aaatatctct aggtgagctt atgcagggtc taatgctagt atctggaaat
     3601 gatgctgcta tagccatagc taaacatatt ggtaaaactg aaaagaattt tgtaaatatg
     3661 atgaacaaga aagcagaaga aataggaatg attgatacgt attattttaa tccaaatggg
     3721 cttcctatat atactgaccc agaacataaa gaacctccta ttgaaaatat gtcaactgct
     3781 cacgatatag ttacacttgg aaagtatatg tacgaccatt atgaaaatca agttacaaga
     3841 ataaccacta tgcaagtata taatgatact aaaaaggatt ttacacatta taacacaaat
     3901 cctctgcttg tttcagtacc tggtgttgat ggaataaaga caggatatac agataatgct
     3961 ggatattgcc ttgcattttc tatgatggta cctaaagatg ctaaaaacga aagaaatcat
     4021 agattgatag gtgttgtact tggtgatggt aacaagaaaa atagaatttc ttcttccgct
     4081 actttattga aatatggtaa agataatttc cattctaaaa agatagcaca taagggtgat
     4141 attatagaga ctccttgtgt tgatggtatt gatgatttta agataacagt taaggttgat
     4201 aaagatttgt atggtgttgt ttcagataac gaaaatatca atcctaaggt agtgtttaaa
     4261 aatatgaatt atcctatcca taagggagat attgttggtg ttgtaaagta ttataatgac
     4321 tctggtaaat ttgttggaag tgttgatgta aaaagtgaaa gtaatatagg atgtattcct
     4381 ttgaaggata agattaagat taaggttgct aagataaata aaaaacttga gattaaaaat
     4441 tctgtttgtt ttaaagcata gtaaagcata ttattaaaag ggtatgttaa atttataaag
     4501 actccacttt gtggagtctt tttgtttttt acaaaaaatt aaaatagtgg caacaattga
     4561 ggatgtgagg gagagttgcc actattttgt atagggtatt atattatgtt tttaagctag
     4621 ttttactatc ttctatgttt atataataac tcttgtgtgt gaatttaatt gataattaat
     4681 atgaaaatta tgtgaagttt taagttagtt gacttagact attgtaaatt ttttcagaaa
     4741 aattctgttc ataagtctat tagaccaccg aattatttac ctataatatt ccgatcaaaa
     4801 aatctattct tttttaaaag taaggtcatg attattgtac atatataaaa attccgaaaa
     4861 ttaatctaca cactagtaac attaagtata ataaatttat tttagtaaaa ctattaaaat
     4921 aacctcataa gaaactatag attaaatcaa tcaatctaat ctacagtttc ttgattattc
     4981 cttaatattt ataatttaca ttatttgttt attttaaaac tatctgttac aaatctagtc
     5041 aatgttgaaa ataaattcgc tctgaatata gtagatgatg
//
LOCUS       DAMJWU010000179         5017 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMJWU010000179 DAMJWU010000000
VERSION     DAMJWU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.184"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(58..495)
                     /locus_tag="PC460_003578"
     CDS             complement(58..495)
                     /locus_tag="PC460_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF2602422.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(752..901)
                     /locus_tag="PC460_003579"
     CDS             complement(752..901)
                     /locus_tag="PC460_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF2602423.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(914..1144)
                     /locus_tag="PC460_003580"
     CDS             complement(914..1144)
                     /locus_tag="PC460_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF2602424.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(1962..2183)
                     /locus_tag="PC460_003581"
     CDS             complement(1962..2183)
                     /locus_tag="PC460_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF2602425.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2763..3020
                     /locus_tag="PC460_003582"
     CDS             2763..3020
                     /locus_tag="PC460_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2602426.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            3489..4727
                     /locus_tag="PC460_003583"
     CDS             3489..4727
                     /locus_tag="PC460_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2602427.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1929 a          496 c          702 g         1890 t
ORIGIN      
        1 ttaaataggt caataaattt gcaattagtg atattaataa attactttag atttttatta
       61 aatgtttagc ttttctttaa tattttttat ataacgacga tttataataa ctttttcgtt
      121 gttttttaaa gtagcttgca tgcgattatg tgatattact tttatacttt ttatgtagtc
      181 catatttaaa atcatggatt tagatattcg tgagaaggat gtattagcta ataattttga
      241 taatttatat atttttaaat caatttcaaa tacatcatct tcataataag caaatgtttt
      301 atttccaact gtctcaaagt ataaaatatc acagacattg agtttataaa tctctcctga
      361 ttttttacct tttatatcaa gtaacggtgt actcaataat gaaattattt tttcaatgtt
      421 attatctaga gcagaacatt ttatggttat ttcaatatca tcacaattaa taccttgttc
      481 gacatgtatt ttcattaatc atcacctcat tttattactg tcattagaat agcattatat
      541 cttattgagt ccaactagat gcgttctaaa tttcaaatta agcatagtga aattcaaaaa
      601 taaaacagtg aaattcaaat ttgaggtggt aaaattcaaa aaaatactat ggatatttgg
      661 ataaattatc caaatatcct agtatttcac atagaatatg tctaattatt ttacaaatag
      721 acctatttta tgacttacat atattgtcaa attacttctt aaatatcaaa atatttttgg
      781 ataatgtatc aatactgttt gataaagtat ttaatttacc ctcaattcta accaataagt
      841 acatggatag agctatagga aaaccaacat tggttattaa cagttgtaaa ctatcatcca
      901 taaaattagc cccctattct ataactaagt cataaggagt ggtatttgtt tccacaacct
      961 tggcttcaac aacagaagct aggtcaaatc ctccaaccga aaaaatattt ttagatacta
     1021 tcaaatccat gacagatttt atattttctt cagttaaatc atcctttgga ttttctatag
     1081 aaaaagaaac ttgtttatcc tttacatttt taaagctcat gactaatttt ttcttaattt
     1141 ccatataatt acctccgata taataattta tattttaatt gtttgcttgt taatattggt
     1201 attttaattg tcaatattaa tgttttgctt gttagtatta atatttcaat tgtcaatatt
     1261 aatgttttgc ttgttagtat taatatttca attgtcaata ttagtgtctt gcttgttagt
     1321 attaatattt caattgtcaa tattaatgtt ttgcttgtta gtatcagtgt ttcaattgtc
     1381 aatattagtg ttttgcttgt tagtattaat atttcaattg tcaatattag tgtcttgctt
     1441 gttagtatta gtatttcaat tgtcaatatt agtgttttgc ttgttagtat tagtatttca
     1501 attgtcaata ttagtgtttt gcttgttagt attagtattt caattgtcaa tattaatgtt
     1561 ttgcttgtta gtattagtat ttcaattgtc aatattagtg ttttgcttgt tagtattaat
     1621 atttcaattg tcaatattag tgttttgctt gttagtatca gtgtttcaat tgtcaatatt
     1681 agtattttaa ttgttgactc tagttattgc tgatatagaa aatatttaat ttatttcaat
     1741 taattagatt aaagtagggt agcatctaaa atagctaaca tgtttaagtt gaaatacgtt
     1801 taatatatta atttttcaaa gcattaatat attaaagtta taatcaaaat tgaagttata
     1861 atctgccaaa tctatagcgt attaagtgcg ttatataact taatctttta tataaagagt
     1921 taattttttt aatttgtaaa gcagagtacg tttaaaaggt actatttaga taatgatgta
     1981 ctatctaatt ttaaaatatc tacaataggg ttctcttgaa gacttgctat tgcttctgca
     2041 acagcataaa catcttcatt taatgcctct gcttttacat ttgcaaatga tttactttta
     2101 acaactgcct tacccttatc atcaaaacca gcatccaatt ttagttttat actagataaa
     2161 ttttttgttg aaacaacttc cataaaatta cctcctaaaa atttaatttc aaagatttgt
     2221 cgactttctt tgttagctat aatactatca tctaagataa tatcggagtt aaacagtaat
     2281 ttctaaagat tttttgtgta aatttttata ggaaaaattt tacttttttg ctgatgaaaa
     2341 agtttacaag ttatttgtga agatatgttc ttagtgtttt gaaatagtat ttgtatatat
     2401 tcatgcttag ttgagatagt tttgtatgga ttttcatatg tgtagagtta aatgttaggt
     2461 actaaagttt atgttaaatt aaatgttaaa gcttatgtta aaaattttat taaatttaat
     2521 ctgtaaataa aaaagttaaa agtccaatat taacatttta cttttaaacc attaaaatct
     2581 ttattaaagc taacaaagtt ataagttatg agaaatttta ttaaagttaa tacaaaaatt
     2641 aattttaaag ctaagaaatt ttttattaga ttcaataaaa ctaataaata tagtttagtc
     2701 aataatgtat taaataaaaa ctttgttaag tctataaaaa tatcttaaag gaggtgtaac
     2761 ttatgaagat aaaagtggag gatataccag ttcaatttca tgcaatggta gaaataattg
     2821 gaattgataa gtttatagag gttgccaagt tgtatggagg aacaaatact tatataccta
     2881 cgtataaggg gctttttaga tatgctagaa atagggatat agtaaaacaa tttaatggag
     2941 tcaaccacaa tgaattggct attagatata atatgtgtgt aagcaatatt aagcgaatac
     3001 tcaatgaaaa taatgtttaa atataataaa ataatgttta aacataatga aaatagcctt
     3061 gttaaacgta agagtaaaga caaagaagat gttttataaa gtttttttag acaaattaac
     3121 taaatttact acttattaat ttaaatacat tgtatattct ataaaaaaat ttgttaaata
     3181 ctggagatat attgagaata caaagaaaat ggtcgaaaat atttgttggc taaaaaatag
     3241 ataaaatgat acaattgctt gtataaaggg gaaaaataaa taaataaact atataaataa
     3301 agaaatagca attaatatga cataatatat ttataaaatt aagaatataa atagatattt
     3361 taaggtatta gttgttaaat taggaaatat taaaaaaaat atatatttat atttaaacta
     3421 ttttgttatt tatataaaaa tacttatcta gttactagca attattagta gaaatggggg
     3481 aaatagagat gaataaggga ttttacaaat taaattcagt ggtattgaac tttgatatta
     3541 agaatgatgt taacaagata agatttaatc attatataat gagaaaagaa aacatacaaa
     3601 gacttcaagg aactctgcca aacggacaat tttatatgac agtgagaaga gctgctacag
     3661 atttacaact ttctatttct actataagta ggcttgtaaa agagtttttg gatttaggga
     3721 taataagatt agtgtctaag ggtgtaaaag gtaatcgttc tgtatatagt tacgtaagta
     3781 tagagtttaa tggaagttta tttgatgata gcaaatatat ggaacttgac tgtgtatgta
     3841 atgatgaatt tattgataat agtggcatag agtttgattg tgatagcaaa gataattctt
     3901 ttaatggaga tataaatgta gaaactgtgc attgcattaa tgagtatttg tctgatgatg
     3961 atgtagagca taattataac aatttccata ataaagatac tataaaggat aaagccattt
     4021 ctaatattag cactatcaaa aaatatattg atgaatcttt tgataaaaat aaatcagaaa
     4081 attctaactt acaacaggag tcaataaatt tcaactttaa tgatacatgc aatgaaatag
     4141 ataaaagaac gaataatagt atagaaaata atcaaaataa aaggcctatg agattgagtg
     4201 acatcagtct tttaagtgat aaaggtatga caaataaaaa gaattacttt aatcataatc
     4261 atggtataca aaaagggact aaatctgtaa caaagaaaaa agaatcctta aataaattta
     4321 ataaaaaaaa tatacagcaa agcattataa atatgctaaa taaaaaatca ggtaagaatt
     4381 ttaaaatcga ttctccaata agcataaaac ttatcaatga aaggctaaag gaaggctatg
     4441 tcttagaaga tttctacaaa gttattgagg taaaagtggc taagtggaaa aataccataa
     4501 tggaaatgta tataagacca gaaactttgt ttagctataa gtttgaatcc tatgtaaatg
     4561 aaaacatatc tatgaaaagt caaagtaatg ttcacccatg taaagaaggc aatagtaatg
     4621 atagcagtaa ttatatacat tctgattata caacatatga tggcagtgtg cgtgagagaa
     4681 aaaaatatga gaatgtaaga tactggaatc ctatatgtga aatttaatta gtttaagaat
     4741 gtatttatga taaataagat attaatagag aatagtgagc tatagcaaat gatatatcaa
     4801 tagttggtag taagttatat taagcaagat attactatca ggtagtgaat tatattgaac
     4861 gatatatcaa taattggtag taaattatat taagtaagat attactatca ggtagtgaat
     4921 tatattgaac gatatattaa taattggcag taaattatat taaataagat attaatatcg
     4981 agtaatgaat tatattgaac gatatatcaa taattgg
//
LOCUS       DAMJWU010000180         4949 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMJWU010000180 DAMJWU010000000
VERSION     DAMJWU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.185"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            61..672
                     /locus_tag="PC460_003584"
     CDS             61..672
                     /locus_tag="PC460_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF2602428.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            970..1308
                     /locus_tag="PC460_003585"
     CDS             970..1308
                     /locus_tag="PC460_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2602429.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1715..3151)
                     /gene="miaB"
                     /locus_tag="PC460_003586"
     CDS             complement(1715..3151)
                     /gene="miaB"
                     /locus_tag="PC460_003586"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF2602430.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(3399..4859)
                     /locus_tag="PC460_003587"
     CDS             complement(3399..4859)
                     /locus_tag="PC460_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF2602431.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSA
                     RLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVN
                     TVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTL
                     VNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLV
                     NDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGM
                     YKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYI
                     KIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLG
                     NAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT         1668 a          755 c          544 g         1982 t
ORIGIN      
        1 atatattatt gttaaaactc aattatataa tttttaataa tattatttta ggaggtaaaa
       61 atgataaacg atattaaaaa taaattcaaa aattacaaac catatataaa tggatacaag
      121 aatatgaaga gagcatctgt tttaattcca atagtagaaa taaataacac acactatata
      181 ttatttgaag ttcgctcaaa aaatttgaag catcaaccta gtgaaatttc ttttccaggt
      241 ggtaaaatag aatcaggtga gactccatat gaagctgtta ttagagaaac ttgtgaggaa
      301 cttggaactt ttagtgacaa tatagaagta atatcaagtc ttgacctcct tataacacca
      361 gtaaagttta tcatacatcc atacttagga tatataaaaa atattaataa tttaaatata
      421 aacaaggatg aagttgacca tacttttttg gttcctataa aatatctttt agagaatcct
      481 ccaaatactt atagtaatac tgtaaaagtt tttccagacg aaaatctacc atatggttca
      541 attccaaacc aaaaagatta taaatttgaa agtggaagtt atcctattct gttttataaa
      601 tataaagatt atgtaatttg gggaatgaca gcaaaaatac tagaaaactt tttaaatttt
      661 ttatcagaat aacactcata ataaatcatc aaaactttta cataagaaat ttataaaagt
      721 ttctaaattt aacaattcta aaatatcaat aactatattt tgtagcactt ttttatttta
      781 ttattgtttg aatatttgat ggttttcaat gcaatttcaa cataaatact atgtattagt
      841 gtatactgtt gatgaattaa agtatgataa tctatttaat aaagtatgaa gtaaatgatt
      901 ttaatgtgcc tttatggtaa aattactaat aagtaataaa attaattaaa atatatggag
      961 gtgcttataa tgtttgctgt aggatttgtt gttataattg cacttgcctt tttcatttta
     1021 agtgtactat tatttaatgg aaaagccagt atgctactag caggttataa ctctatgagt
     1081 aagaaagaaa aagcacgata cgataaaaaa aaattatgta gaagttctag cattgttact
     1141 tttattgtct ctataatgct atttattatg gcgtttttgg gatatagaat agagagtggt
     1201 caaatggacg aaaaaaaaat ggttcccttt tctataatat ttataatagt catactttcc
     1261 agtgtatttt ttaatatcct ctactctaac aaaaaatgta aaaaataaat agttgtaagt
     1321 tgagcagtta attataaaag attgactgct ttttcatgta tattgactac tcttattaaa
     1381 tattttaaca tttaaaaatc tatgtaaaaa taatgaatat agtacaaact tctaataatg
     1441 attttacctt tgtggttaca gtccactata tataatttaa tatgataaca gggaattact
     1501 aatgacaact aggtaagctt ttataaaata taataaaaca aaaatagatg tacaatgaaa
     1561 ttatatctta tatcaaattt atttttatta atagcaaaaa cttacattag cataacatat
     1621 gacaaattcg cttaaaataa aaatttttaa tataatttgt tctatagtac atctattttg
     1681 attagtttac ataattttat tattttgtac aatattaatt atttactaat atcccattta
     1741 atgaaaaagt tctaggttca gtaattttta ccataactaa tttaccaatt aaatcatcat
     1801 ttccaccttc gaaatttacg agtttatttt gtctagttct accagcaaac ttattttcat
     1861 tatttttact tcttccttca actagaactt caactattct atctttatat ccatcattta
     1921 tttctgcaga aatcctattt acagcttcta acaccctatt aaatctatca tgctttatat
     1981 cttctggtat ttgattttcc attttagcag caggagttcc ctgtctttta gaataaataa
     2041 atgtaaaagc tgaatcatat ctaacttttt caaccacatc taaagtgtcc aataaatctt
     2101 cttctgtttc tcctgggaaa ccaatcatta agtctgtaga aaaagcaata cctggaactt
     2161 cctttttagc tttttctatt atttctaaat aatattcctt tgtataatgt ctattcattt
     2221 ttttcaaaag gctactgctt ccacattgta ttggtagatg taaaaactca cacaccttat
     2281 cacaatctct aattgcgtat attacttcat cagatatatc ttttggatga gatgtcataa
     2341 atcgaaccct ttcaattcct tctatatcat tcacttttct taaaagttct gagaatgaaa
     2401 ctggattttc aagtgtctta ccataagaat ctacattttg tccaagtaaa gtgatttctt
     2461 tcgttccatt tgcaacaagc tcttttatct cgttaattat atcttcagga gttctacttc
     2521 tttctcttcc tcttgtgtat gggactatac aatatgtgca gaaattattg catccataca
     2581 ttatatttac aaatgctttt aattcaaatt ttctattact tctaagacct tctattactt
     2641 ctccatccac atcccataca tccactaaca ttttgtcaga atttattgat tctgtaagta
     2701 attgcggaaa tttatataga ttatgtgttc caaatataag gtctacatgt ttatattttt
     2761 ttcttaattc ctctacaaca tgtggttgtt gcatcataca tccacataca gctattttca
     2821 tatctggatt ttttcctttt agatgtttta attgtcctaa gttaccatac acttttagct
     2881 ctgcattttc tctaactgca caggtgttat atattattaa atcactttct tcaaccatca
     2941 ttgacatttg gtatcccatc tcttctaaca ttgaacataa tttttctgaa tcatgttcat
     3001 tcatttgaca cccgaaggtt tgagtaaaca cgagttttct ctttccagtt ctgttgaagt
     3061 attcttcatt ttgtctgtgt atttgttcta tatatttatc ttgttcttgt attttctcta
     3121 taggtattgt tacttgtttt cttttgctca cgtctattcc tcctagttaa tttcttatat
     3181 tacacataac cttaattata tcaaataaga aaaatctttt aaagatatta ttatttttct
     3241 agttgtgttt taatagtaaa tctaatgatt tatatatact atatttctaa aatataaaat
     3301 catataatta ttataataat aaaaagatgt tttaaattaa aacttaaaac acctttcctt
     3361 aaataaaata tatgatttac tattaaatac aattacatct attcattttc aattaaataa
     3421 caatcattta atattaaata ttgatcttcc tcaaccgtta tatatcttct tcctgaaaaa
     3481 gtatcattag taactattcc ctctgcatta cctaagctat catttctaac ttcataatat
     3541 gcatcagaag aatttgacat tataatatat tctccagcag gaatttctaa tccaatttta
     3601 tacataccat ttccaaactt accattcttg gccttctggg ctttagcata ctttgctaat
     3661 gtcattgttg aatcctcaat tttaatatat tgaccattct ttaaggtgat atatctagtc
     3721 ccactaaaaa tatcatttga aagtatacta tcagcattac ctgtactatc acttgtaact
     3781 tcataataag aagcataact tcctgagttt gaagttatta agtattcacc tgctgagata
     3841 tcttttccaa ccttatacat tccatctttt atagtctttg tagtaggttt aacatttttt
     3901 acaaccccag tattaatatc tccaattcca tttgttatat ttaaacactg ttgtactata
     3961 tttgaatcta tatatcctat agaagttatc ttcttagcat tttttaaaat gcttttattg
     4021 ctaccatcgt taactaaaac cattggacca tgttttgata aagaagatcc tacaagtgca
     4081 tttgttaatt cataagctcc agctatataa aactctctag catccttata aaacttattt
     4141 attattgctt tattagtttc aaaacgagta ctccccccta gtctagtaga tttagtattg
     4201 tttactaaag tggtactcat tgaagctgtt cctcctatta cataagactt taaaccacta
     4261 gttgaaaaag gtatactttg tccattagta agaattattg gtgctttatc tcttgctgct
     4321 acagatgcaa tacttatagc atctgcttct cctttatatg cattggttag cataacagta
     4381 ttgacttttt ttattgaatt tatttctttt gcaacattat aacttgtttt tattctgtca
     4441 tttcccttaa ttcttacaac tttcattttc ttagatttta atgaattttc gacagatgta
     4501 ctaatagaat atgttcctcc aattatataa acttttgata cattttttaa tcttgcagat
     4561 gtctcagttg gtatagtatt tttttcagtt aatagtatag gggcatttaa agctcctgct
     4621 agtccacttg cacttaatcc atctgcaata gagttactag tattaattaa tatagcagtt
     4681 ttatatgcag ttttatatgc agttttatat gcatttttat ctgctatttt acctgctatt
     4741 tcatacttat tttttccttg tattttttct acagatgata ttgcatctac taattgaata
     4801 tttgcaaata caaaagaaat acttaaagtc aatgttaata ttcttttata gaatttcata
     4861 ataaaattgt ccccctcata ataaattttt aatatataaa tacatattct taaattagtt
     4921 atgtacttaa caaacttatg taaaattgc
//
LOCUS       DAMJWU010000181         4693 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMJWU010000181 DAMJWU010000000
VERSION     DAMJWU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.186"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(166..969)
                     /gene="proC"
                     /locus_tag="PC460_003588"
     CDS             complement(166..969)
                     /gene="proC"
                     /locus_tag="PC460_003588"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2602432.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1294..3663)
                     /locus_tag="PC460_003589"
     CDS             complement(1294..3663)
                     /locus_tag="PC460_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF2602433.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3668..4576)
                     /locus_tag="PC460_003590"
     CDS             complement(3668..4576)
                     /locus_tag="PC460_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2602434.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT         1383 a          908 c          512 g         1890 t
ORIGIN      
        1 tatttaatta ttaatattta agtaataaat tctcactcaa tcattaaatt cctttttata
       61 gtaaattact cttattttac ttataccaaa tacatttatg tatagatata aataagcact
      121 aaaaaaacaa ttcaagaaac catatagtaa acttaattac atctgttatt tactcatatc
      181 tttagactta tctatacaag caacaataga ttttataact gctgctctaa agccttcttc
      241 ttctaatacc ttaacagctt ctatagtagt tcctgctgga gaacaaacca tatcttttaa
      301 ttcacctgga tgttttccag tttcaagaac catcttagct gaccccatta ctgcttgaga
      361 tgcaaattta taagcttgtt gtcttggcat tccagctaat actgctgcat ctgccatagc
      421 ttctataaac ataaatacat atgcaggaga agaaccacta atacctgtaa ctgtatccat
      481 taaatattct gatactattt ctgcctcacc aaaagattca aacagagcct taatcatatt
      541 taactcttca tctgttacat tactattttt acaaagagct gacattcctt cgccaacaag
      601 ggctggagta ttaggcatta ctcttactat ttttttatct gaacctataa aactttccag
      661 actttctata gttttacctg ctgctataga tactattatt ttatttgcat ctattaattc
      721 tttaagtgga gttaaagcct tttcgcatat attaggttta agtgctacta ttaccacatc
      781 agaatttgta acagctacct ttgaatcagt tgtagtattt actccaaatt cttcttttac
      841 tttatctaag gcaacttggc ttaaatctga tacagttata tcttctgcct ttactaatcc
      901 tgactgtact atgccaccta tcattgcact tcccatattc cctgaaccaa taaatcctaa
      961 agttttcata tcaaatctcc ttttttctat tagttttatt ttaaagtagc taaattgact
     1021 ttattttaac tgcttaaaat tatcttattt ttataaaata taaccttatt ataactataa
     1081 taaattattc ttttgtatgt attacttact aatatgtgta tgatttacta aatataaatt
     1141 taacgatttt ttcattaaac ttaacgagct aacttcctat ttagaaagtt agctcataat
     1201 taatctaaaa ttttaaaaat ggaatcaaac catttatact ttattatatc agttacgatt
     1261 ttatatagta agaagtttta tttacaagca aacctagaat gtttgctcag ttcttcctat
     1321 tatctcatct tgtagtgttc tacttaaatt attgaaatgg tcactatatc ctgcaactct
     1381 aactattaag tctttataat cctgaggatt cttttgagcc tctaacagta catttttatc
     1441 aaatacatta aattgtatat gatgaccatc catattaaag tatgctctta ctagattagc
     1501 catattatct aacccttttt ctccttgaac tactgatggt gcaaatcttt gatttaataa
     1561 tgttccacca gtttttatgt ggtccatctt agcacaagat tttataactg ctgtaggtcc
     1621 attagtatct ccaccttttt caggtgaaat tccctctgaa actggttttg cacaaagtct
     1681 accatttgga cttgctccca taatttctcc aaagtatata tgacaagttg ttggtaacat
     1741 atctactctg tattctccac cacaaacagt tggtctacca gttacctcat tgtaatatgc
     1801 attgaatatc tcttgcatta tttcatcagc ataatcatca tcatttccat atttaggagt
     1861 cttatttgaa actaaattaa gtatagcttc atatccttca aaattagcat caagtgcttt
     1921 aagcaatgta tccatatcga attttttctt atcaaataca ttatatttta ttgctgttaa
     1981 actatctgtt atagttccta taccaacacc ttgtatatat ctagtgttgt atcttgctcc
     2041 acctctttgg taatccttgc ctttttctat acaatcatca actattgtag acataagtgg
     2101 acaaggcatg tgttttgcac atatattttc tatcacagca tttccttcta tttttatgtc
     2161 aatcatataa tgtaattgct ttttaaatgc ttcaaatagt tcctcataag attggaaatt
     2221 tctagggtct ccagtttcaa tacctatttg ttttttacta attgggtcat atccattatt
     2281 taatgttaat tcaagtattt ttggaatgtt catatatcca gttagaacat atgcttcttt
     2341 accaaaacat ccagtttcaa cacaaccact ggttccacca agtcttgcat cttctatagt
     2401 ttttccagct tccataagct cttgaactat tgcttcagta ttataaaatg ctggttgacc
     2461 ccatcctttt cttgatattt cacaagctct ctttaagaat ttttgaggag tctttttact
     2521 tatttgaaca tttgaactag gttgtatcaa tttcatttca tccataacat caagtattat
     2581 ataactaact tcattaacac catcttgacc atctggattt attccaccag tgtttatatt
     2641 tgcaaaatct gtatatgtgc tactttcttt taatgttata ccaacttttg gtggtgctgg
     2701 ctgattgtta aatttaaccc ataaacattc aagtaattct tgtgctctat ctctatctag
     2761 tattccattt tctacatctc tttcatagaa aggatttaga tgttggtcaa gtcttcctgg
     2821 agtaaaagca tcccatatat taagttcagt agtaacccct atatgaacaa accaatacat
     2881 ttgtatagct tgatgataag tttctggttt gtgagctggt acaacatcac aagtttcagc
     2941 aattagtaat aaatcttgtt tcttagcttc atctgtttct tcttctgcca tttgtcttgc
     3001 tttctcagca tatcttttac ctaatataac cattgcatca caagctattg ccatagcttc
     3061 taaatctgct tttttattat atgcatctaa atcattaaga aaatctagct cttttaatct
     3121 tgcttctatg tctttcttta aatctaaaaa tcctttttta tatattgtat ctccacaaac
     3181 tgtatgtcct ggggctcttt gttccataaa ctctgtaaac atacctgctt catatgctgc
     3241 taaccactca ggtgtcattg catttattat cttatctctt gtttgtctct ttttccaaaa
     3301 aggaattatt tcttctttat gtatttttct agcttcttct gatacgctaa aatttattat
     3361 atctctattg tgcattactt ctaaatcttc cattgtatga caacaaattt ctggatatgt
     3421 tggtgctcca ttaggagaat cacctttttc acctactatt aattctccat catttatgct
     3481 aagagttcta ttctcaatat aatgcttaaa tgataaagca cgaagtacag gaatctctac
     3541 acttccctca tattttttat aagcttctgt cataagtact gctctttcaa tagatatgtg
     3601 tggttctgca ttaatacttt cttctcttaa ttttttagtt ctctcaaaag ttcctcttgc
     3661 cattttatta ccctccaatc ttagtattga aattacttgc ttcaaacagt ctttttattt
     3721 cctctaattt ttcttctgat ggtctttgta gttcttctcc ttcatacttt ttataaattt
     3781 tatcatactt gtgtttacca atattatgat atggaagaag acttactgct tgtatattca
     3841 aaggctttaa aaactctatc atttttttaa cctctaaatt ttcatcatct acatttactc
     3901 ctacaattaa tggtattctt atatttatgt tagcccctaa ttcacttaaa atcttaacat
     3961 ttttcagtat taagtcattt gattttcctg taaattttat gtgcttatct tcatcaataa
     4021 gcttaatatc atataaaaac aaatctgcac atttagcaac tctttcataa ttttctgact
     4081 ttgcatagcc acatgtatca attgctacat ggattccctt tgatttgcac atgtttatta
     4141 ctccacaaat gaagtccata tcttgagcca ttacttctcc acctgataga gttactccac
     4201 cacctgattc ttcatagaaa attctatcct tctctatctc tttaaccaaa tctcttacag
     4261 aatactcttg accaacaatc tctcttgcat tgtttacaca atagtctaag caagtttcac
     4321 aaaactcaca cttatcttgg tctaagcata tttttgtttc acctttgtaa attgccttat
     4381 gagggcatat atttatacat gcttcacatt ttgaacatct ttcttcatta tacaaaactt
     4441 gttttgtata tgtttgactc tctggattat gacaccaaac acattcaagt ggacaccctt
     4501 taaaaaatac tgtacttctt atccctggtc catcatgtat gctacatttt tgtaagttta
     4561 taactaatgg attcatgatt tttcacccct aaataatatc cattccattt tttcattttt
     4621 atgcaattca tcttcataga atatacctat gaaaagcatt tctacaatta cataatacta
     4681 tatttgttat tat
//
LOCUS       DAMJWU010000182         4551 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMJWU010000182 DAMJWU010000000
VERSION     DAMJWU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.187"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            227..1039
                     /locus_tag="PC460_003591"
     CDS             227..1039
                     /locus_tag="PC460_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF2602435.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1128..1319)
                     /locus_tag="PC460_003592"
     CDS             complement(1128..1319)
                     /locus_tag="PC460_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602436.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1590..1829
                     /locus_tag="PC460_003593"
     CDS             1590..1829
                     /locus_tag="PC460_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2602437.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            2138..3757
                     /locus_tag="PC460_003594"
     CDS             2138..3757
                     /locus_tag="PC460_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2602438.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDEHFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            4131..4325
                     /locus_tag="PC460_003595"
     CDS             4131..4325
                     /locus_tag="PC460_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2602439.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT         1706 a          521 c          627 g         1697 t
ORIGIN      
        1 tttctatata tttatatgac taagttctat aactaaattg aaataatcac tttttcaaga
       61 atgttataat ttttttattg tattcttttt gctatagtct atgaataaat caatagaaaa
      121 tcttctaagt tgcttttaaa taatgacttt atggttcaat tgtactaaaa tagtatatgt
      181 aggttacaaa taatctaata aatattaaag tttggaggta atagatatgg cagattgtaa
      241 ttcatgtcca tcaaagggaa attgtaatag tcaatcaaat tgttctattg aaaataaccc
      301 taacaataaa tttggaaaaa taattggtgt tatgagtggg aagggtggag ttggaaaatc
      361 aactgtaact gctttacttg ctaataagct aaacaaaatg ggatataagg ttggtatatt
      421 agactcagat ataactggac caagtattcc tagacttatg ggtgtaaaaa atgtaaaagc
      481 ttattctgat ggctcttata tttaccctgt agaaaactca aataacataa aagtaatgtc
      541 tataaacctt atgatagatg atgaaaatga acctgttgtt tggagaggac ctttacttgg
      601 aggggttgta aagcaatttt atactgatgt actttgggaa gaattagact atttattaat
      661 agatatgcct cctggtactg gagatgttgc cttaacagtt atgcaatcta ttcctataag
      721 tggaatagtt atggtttcag tacctcaaga tttggtatct atgattgtat caaaagctgt
      781 taatatggct aaaaaaatga acataaatgt ccttggagtt attgaaaata tgagttatat
      841 ccaatgtcct gattgttcta aaaaaatcaa gttatttgaa ggagaatcta ctgaaaagtt
      901 tttagatgat ttagacttag agcttttagg agaacttcct atgactaaag aaattataga
      961 tattactcat aatggagtta cagaaattag tgatgattta gattcaatat tgactaatgt
     1021 tgttgaaaaa attaaataaa taaaaatttt ataattttat atttatcaaa aaaagaggct
     1081 tgtatttatt ttataatcat acaaataaat ataaacctct tttatgttta tctatgtttt
     1141 aaatacacaa aatattcttc tacttcatct ccatttttta cttttactaa atattcatta
     1201 taatgagcat tatatggctg tgtcattttg cattctatta cattttctat gtcttctaat
     1261 tctaaaagtt ctacatcctt taaaaatttt ctttctatga gttccattaa tctttccata
     1321 atatatcctc acttccccta taattaagat aaactatata tcctcttaat gcagagttta
     1381 tctgtttatt atattatatc caatatatta cagtttctaa catcattcat aaattatttt
     1441 aacaaataga gattgatttt ttaataaata tgccttgata aaatgaaaca tagaccaact
     1501 cttagtttta tgtaaagaaa acttaaagaa aactataaat tatattcaca aaaattactc
     1561 agaagatata acagtaagta atatatgtaa tgaattacaa cttaataaaa gttatttttt
     1621 ctttgtttaa gaaaaaaagt ggatatactt ttactaattt tttaaataaa gttagagttg
     1681 aaaaaagcaa aaagtatata ttaaaaaaag acttgtctat tttagacgtt gctgtactaa
     1741 ttggattcaa tagtcaaaat tactatagta taatttttaa aaagtttaat aactttacac
     1801 caattgaata taaaaatatt tataactaaa aaatatcatt ctaaaaggat gttatttttt
     1861 tacatatttt ttacaattat agttaattct ttgttaaatt gtttgtacat aaaatactaa
     1921 aaatctaagt tcaagtattt aaattgctat atatcataaa ttttctaaaa ttaacaacaa
     1981 cttaaaaatt taacattctc ttaacgtata cttaacaaaa cattaatatt tttattgaca
     2041 aaatttgtta ttctatatta tggaaacaat aaattttagt taataaagtt tataattcag
     2101 ataaaaaaat tattaatttc ttaagggagg aaatatcgtg aatatagtaa ttagccttat
     2161 cggtggactt ggactttttc tttacggaat gagtttaatg ggagaaggtt tacaaaaatc
     2221 agcaggagac aaattaaaaa aaattattga actcttaaca agtaatgttg ttatgggtgt
     2281 actggtaggt acagtcgtta caggtattat acaaagttca agtgctacta ctgttatggt
     2341 tgtaggattt gtaaatgcag gtataatgaa tctaagtcaa gctattggtg taataatggg
     2401 ggcaaatata ggtactacag ttactgctca attagtttca tttaatcttg aaggaattgc
     2461 accaattgct ttaggtatag gtattttatt ctatttattt acatcaaatc aaaaaacaaa
     2521 gcatttatct gagatactta taggttttgg tatattattt acaggaatgg agtttatgaa
     2581 agatgcagta gctccacttg ctgaatacaa agcattcact gatgcacttt tatattttag
     2641 taaaaatcct gtattaggta tattggcagg atttgccata actggtattg ttcaaagttc
     2701 aagtgcatct atgggtatgt taatagcact agcttctcaa ggaatacttc ctctatcatc
     2761 tgctttacct attctttatg gagataatat aggtacttgt gttacttcac ttttatcaag
     2821 tgttggtgca agtagaaatg ctcgacgtgc agctgtaatg catttaagtt ttaatgtaat
     2881 tggtacaata atatttatgc ttgttttaaa taaacctata tcagccatag ttactcattt
     2941 tgacccaact gatacagcta gacaaatagc aaatgcacat actttattta atcttacaaa
     3001 tgttataata ttattaccat tttctaaata catagttaaa ttagcaaata gacttatacc
     3061 tattaaagaa actgaatcag aaatagtaaa taatactaag tacttagatg aaaggatgtt
     3121 tagcactcca tctatagcac ttggtaatac tgtacaagaa gttgtaagaa tgggtcataa
     3181 agctacaaac tctttggaac attcaattgc aggattttta aataagagca atgaagatat
     3241 taataaagct tttgagtcag aaaaagttgt caataagtta cagaaagata tattgaacta
     3301 tttgttaaaa ctttctaaag aacctcttag agatgatgaa cactttagaa cagatttgct
     3361 ttttaatact gtaaatgaca ttgaaagagt ctctgaccat gcagaaaata tagctgaatt
     3421 ggccatgagt gttaaggaaa tgaacattag tttttctgat agtgcgataa gagaaatata
     3481 cgaaatatac aataagacaa ttactaattt taaggatgcc ttaatagttt tagatgttaa
     3541 agattttgag ttagcaaata aagttttaga agttgaaaat gaagtaaatt atttagaaaa
     3601 aacttttaga aattcacata tgattagact aaataatgga agttgtacaa ttgatgctgg
     3661 tgtactttat cttgacttac ttactaatct agaaagaatt tctgaccact caacaaatat
     3721 agtaaaacaa gtactaaaac ttaaacaaaa aatatagtga ttatttttat atataatctt
     3781 aatcaatgag aagtaagaag ctatagataa tgcttatatc atttatggtt tcttattttt
     3841 tatgtaatac tcaaattttt atagtattct cttattatta aacaatttct gcctagaata
     3901 ttccatatat atatgacttc tttcataaaa cttgaactgc ccataactaa agtcacaagt
     3961 atacataact attttttaat caattataac caatatttgt tttatttatc ttcattttaa
     4021 ttcttcattc aataatgtaa taagattatt ttttatttgg atactattat atcatctaaa
     4081 tatgttatta tatatttgga tattttcata ttaataggag ggattctaca atgataaatt
     4141 atatggtaga aattatatat agcgaaaaaa taagcgaagt agaaaacatg ttgaaaattg
     4201 aaaatataca atatgaattt acttctcgtg atggctttat attttttggt ttttatgata
     4261 tgacagacgc atatagattt gagactttat gttcagatga aaaaattcga tttaaaagaa
     4321 tataatagat aaaaaagtac atatttctaa actatttgtt tttcaaattt aggttgtgtt
     4381 ttaatgtagt gttgatatta taaacaattt taataataaa aaagtaggga cattatatta
     4441 atatgctcct actttttact catacttatt aatattattt atcaaaatta ccctacaaac
     4501 tggaatttat cattctttca taattttaaa ctatcatatt taaaatatat a
//
LOCUS       DAMJWU010000183         4447 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMJWU010000183 DAMJWU010000000
VERSION     DAMJWU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.188"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(894..1304)
                     /locus_tag="PC460_003596"
     CDS             complement(894..1304)
                     /locus_tag="PC460_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602440.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1520..2902)
                     /locus_tag="PC460_003597"
     CDS             complement(1520..2902)
                     /locus_tag="PC460_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2602441.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNGKDVLYTDSGEVSIEQSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2913..3272)
                     /locus_tag="PC460_003598"
     CDS             complement(2913..3272)
                     /locus_tag="PC460_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2602442.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3477..4256)
                     /locus_tag="PC460_003599"
     CDS             complement(3477..4256)
                     /locus_tag="PC460_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF2602443.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTVISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1548 a          750 c          399 g         1750 t
ORIGIN      
        1 actatctatt ttttcttact attcttatcc acatcatcca tatatttatt ttacgattac
       61 tctttcactc taacccttta actatctatt ttttctgata ttcttatcca ccatcaccca
      121 tatatttatt ttacaattac tcttttaccc taacccttta actatctatt tttttctggt
      181 attcttatct actattatct gtatatttat tttatgatta ctcttttaac ctacccccta
      241 tactttatag taatatgttt ttaacctttt tcattattcc caatatgctt tataactaca
      301 aatactctcc ccccttaaaa ataataataa aattaatcaa taaatttata atcatattat
      361 tactctatgt attaagcaat tactatgcca ataaactttg atttttttaa acaaatctta
      421 aaaatcctaa aatgctaatt ttctttaact tttaatgaat ctaatagata acatttaatt
      481 ttcgttatat ttttcagaat atttctatta ttatttttaa gaaaaaaaaa taatgttaac
      541 atttcttaac atacattaaa aaatgttaac attataaatt gggtttaata cttttacttt
      601 gactcgattt acttttattc tacttcttac tttattttta acttagactt tattttttta
      661 ttacttattt gagtcttatt tattttatta tcactccata tgttcaatta catttgtcta
      721 aattctttga tacttttatt acataatttt aacaagctta agtgttattt gggtagtgca
      781 gttgataatt atttaaataa tcatcaactg catgtttata tcattacagt agcttaatgt
      841 atttaatcat acttacttag gattttgaaa tatcttagag attatattta acattagtca
      901 attgctgcat aattgtaagc aattgaaatt tgagacccat cttcattttc tatagtgata
      961 ggcttcataa tttttaatcc ttcagttttt atcttttcta attcttgttc cattttctta
     1021 attgtttcct caaggtcttt ttgacttaca tctttgactg cattagggtc tttagcatag
     1081 tcttttaatt cttgaatatg tttagcatac tcttcataag tataatgctc aacaccatac
     1141 ttttccaatt cagtagggtc agttattcct gattttatta gagattcaag tttaggtaaa
     1201 gcaatattag atgaatcatt tccatttgca aatactgctc cagttcctcc tagtaccatt
     1261 gcagatacca tcattgcact tataagggat ttttttattt tcataaccaa tcttcctttc
     1321 ttagagattt atttgggtag tgtagtttga aatgactatt gcaatagtta tcaaccacat
     1381 ccttagacta tcacagtagt catgtttatg tcaagccttt ttaaaaaact tttcaataaa
     1441 aaatactatc tcaaaacaaa attaattatt ttgagatagt attttaattc taaatctttt
     1501 atattactaa gatttatggt tactctatcc atgttctata ttgttcttta ttaactgcta
     1561 acaaattccc ttttcttgat atgacaaagc gattatcatt atagccactt tgctcaattg
     1621 aaacttctcc tgaatcagta taaagtacat ccttaccatt actagattta tttacatacg
     1681 tttcttcgct attctcagaa ttagttactt tgctcataga tgattcttca tgctctctaa
     1741 attcaattac agatactttt gccgtactaa aatgagtctc tccatcactt ttagctttac
     1801 taacaagaat atcagattct actttgggag aatataaatc agttgattta ctacttattg
     1861 aaaatccaat tttatcactt tgttcatttt ttgttaaacc aaaagattca acctcattaa
     1921 tctctttatt ttctgttgca aaaacaagac ctgtcccaca aactaataca gttgaaacta
     1981 aaaaagtaac ataagttgat tttttaaatt tcataatatt aactattctc ttttctattt
     2041 ttgtctttcc aaatccacta catattgtca ttgaaaatgg attattctcc tctgctagac
     2101 ttattagtgt ggttgcataa tcagccctat attcttcacc aagtatactc ataacctttt
     2161 catcacaact aaattctatg tctttattta gtaaaaaaaa catcacccaa actaagggat
     2221 taaaccaatg gacaacaaga gctacaagca taaataactt taacagatta tcaaagctat
     2281 taatatgaac catttcatga gttaaaacaa agtctaaagt ttgattatta gcataattca
     2341 tttgctttgg caaaataatt cgtggtttta aaataccaga tgtaagtggt gtcctagtaa
     2401 attcagatat gcaaacaata aatcttctaa aacggctatt cttacacatc cattttttaa
     2461 tgaactcatg attttttata tgaattgaag ttttaaaaag attaattatt ttaaaatatc
     2521 taaagcataa aagtatgcta aacataatag taactataaa agacaaaatg cccaacattt
     2581 ccaacatcat aatattttca atattagaca ttatttcaaa ttcgtcagaa ttgtttttac
     2641 ctgtagagtc actagctttc ttaagagtat aattttctgt tgatgtatta cccaattcca
     2701 catcaaaagt cgattcaatt atactataaa tactaaaacc agaatttaaa cttactggta
     2761 ttagaagttt aaataaaaca atcaaccaca ataatacaaa taccttttta ggtaattttt
     2821 taaataaaca tgctcttata aaaataacta ttaaaagtaa tactccagaa gtaaaactca
     2881 tttctaacaa tttaatgaac ataaatctta cctcacttta atttttcaac aatttgttta
     2941 agttttgtaa tctcatcact tgaaagattt tctttattaa taaaagctga aagaaatttt
     3001 tctgaagacc ctccaaacat tttattaatt aaagtttctg tttcctgttt ttgtacttcc
     3061 tctttgctta ttaaagcttt acaaacaaaa ttcggctcaa ttctctctat tgcacctttt
     3121 gacactaatt tttttataac ggtatacgta gtgtttctat tccatcctgt ttcttctttt
     3181 aaaattttta ctaactctcc agccttgctt tctcctctct cccatagaac ttccattact
     3241 ttaagttctg aatcaaataa ttttatctcc atataaaatc gctcctcaaa tatagttatc
     3301 acaatagtca ttactataat aaaaaactat catagtagtc tatactatgt caatcattta
     3361 ttttttaata ataaaaactg tctttttaaa aaattcttat aaaagacagt tttatcaatg
     3421 ttatacttta atctttaatt aaagccatat tttatttaaa tatataacta tatatcttag
     3481 tacatagtat aagatttttc tttatctaat agtatatcct ctacatctgg gttttctttt
     3541 actaattgtc tataaaaatt acaattagta acacctacat atggaattag ccatagtaga
     3601 cctattccaa gagtagctat tgaaagtata taccatccta taaaactaaa gtttagtaca
     3661 aataatttaa gtacatttcc tttcatcata tctaaactag cacccattga tttaaataca
     3721 cccatattat ccttgtcttc acaaattaag aaagtagctt gagaaacata taatagcaat
     3781 atataaaccg gtaacataag aacaagtatt ataagaacta gagttattcc agtagaaata
     3841 acagtcccta ttccaacatt atcacttgat aaatattcaa ttgctccact agtaagtaaa
     3901 actccaaatg ataaaaacgc tcctataatt gtgattataa aagaataaat ataaacaata
     3961 attgattcaa ataaataaaa accaaaactt cttaaataaa ttctcccact tacaaagata
     4021 tctcctatct tgactttttc atctttagta attttcaaga acatacatgt aaatcctgct
     4081 gaaacgcaaa aagctattac agccactaca atagtagtca atggttcaaa aggtaatcct
     4141 acaattgata ttaatactat caatgcaata tatactgcca tagaaattgc acatatcccc
     4201 cagtttccag ataactgagc tttagatact cgttttaatt cttgccttga taccataaaa
     4261 aacacccctt tcattttata aaaaaacttt taaaaatata aataatcttt taacgtataa
     4321 tattaatata tcatattttc gactatataa gatacaatta ttcaccatgt tttatcaaga
     4381 tataccataa ttgtattact agttttatat agtcaacatt accaaataca tcaaataaat
     4441 ttgatta
//
LOCUS       DAMJWU010000184         4400 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMJWU010000184 DAMJWU010000000
VERSION     DAMJWU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.189"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            156..1631
                     /locus_tag="PC460_003600"
     CDS             156..1631
                     /locus_tag="PC460_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2602444.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1718..2131
                     /gene="speD"
                     /locus_tag="PC460_003601"
     CDS             1718..2131
                     /gene="speD"
                     /locus_tag="PC460_003601"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF2602445.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2161..3012
                     /gene="speE"
                     /locus_tag="PC460_003602"
     CDS             2161..3012
                     /gene="speE"
                     /locus_tag="PC460_003602"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF2602446.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3002..3880
                     /gene="speB"
                     /locus_tag="PC460_003603"
     CDS             3002..3880
                     /gene="speB"
                     /locus_tag="PC460_003603"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF2602447.1"
                     /translation="MKNNFYHMNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4046..4246)
                     /locus_tag="PC460_003604"
     CDS             complement(4046..4246)
                     /locus_tag="PC460_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2602448.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1732 a          494 c          758 g         1416 t
ORIGIN      
        1 tgtttttgag taacttcaaa aaaacacttg taaaaatgct gggatgttaa tataataaat
       61 atcatgagtt taataaaagt aaaaataaaa aatagaaata ataaataata aatattaaaa
      121 atatttgaat atagagagga gatgaatttg aaatcatgga tagcgagttt gcaaatgcag
      181 ttgaattaag ctatgagtgt gctccattat tagagtcgtt aaaggaatac tcagagaaag
      241 atatagcttg ttttgatgtg cctggacatg taaaaaatag aggtgtagct atattaaata
      301 aatatctagg tgaaagtctc atgaaaatgg atataaattc atcaccaact atggataatg
      361 tatctgcgcc aaatgggatt ataaaaaatg cacaagattt gttagcacaa gcatatatgg
      421 ctgatgaggc attctttata acgaatggca caactcaagc aatacatgca atgattttga
      481 gtgttataaa accaggtgaa aaagtattac ttccaagaaa catacataaa tcagtaataa
      541 atgcgttaat actttgtgga ggaataccaa tatttataca accagaattt gatgaaaaac
      601 taggtataag tttaaatatt actcttgaaa aagttagaac agagatagaa aaagattgta
      661 atataaaagc attatttttc cttaatccaa cttactatgg agtttgtgca gaccttgaga
      721 gtattataga actatgccat aaaaataatg ttttagtgtt agttgatgaa gctcatggtg
      781 cacattttcc atttcattta gatttacctc cttctgcaat aagtttggga gctgacatgg
      841 tagctgtaag tatacataaa actggaggag cattgacaca atcttcagca ctcttattga
      901 atagagataa tgtaagtttt gaaaaggttc ttcaatcaat caatatgcta caatcaacat
      961 cagcatctta tttgcttatg gcaagtatag atggtgctag agttaatcta gttgagaatg
     1021 gtgaaaagca attgtcaaaa gcattaaatc tatctaggta tgcaaaatct aaattgaaca
     1081 aaattgatgg aataaaagtt ttatctacag aaatattgaa acaaaaaggt gttaaattta
     1141 tagatgaaac aaaattgtgt ataaatgtaa aagaattaaa tttgactgga tttgaagtat
     1201 acgatttatt atataaaaat ttttcaattc aagtagaatt gggagattca tataatatac
     1261 tggcacttgt gtccattgga acaaataaat cagatataga tagattagta aaggcacttt
     1321 ctataattgc aaagatttat agaaaagaat caacattaaa tgaatttaat atggttcaaa
     1381 taaatccaat tattaaactt aatccaagag aagcttttta tgcaccaaag gaaagtgtag
     1441 aaatcaattc atgtatagat agaatttgtg gagaatcaat aatggcatat ccaccaggta
     1501 taccgattat tgctccagga gaattgataa cagaagaaat tatggagtat attattttct
     1561 taaaaaatag caatgcatat cttacagatg tacaagataa aaacttagat agaatactag
     1621 taataaaata aattacttta ttttgcaagg gcaagtgttt gttaagtaga gtttatcaaa
     1681 tagaacttat taaaagtata aaggagttgg ggttgaaatg aaatttgaaa cactaggaag
     1741 acatatacta gtagaatact ataattgtga tgaaaacgta cttaaagacc cacgtcttat
     1801 agaaaatttt atgaatgatt cagcattaaa tgcaaaagcg acaattgtgg attctgtatt
     1861 ccaccatttt aatccttggg gggtttcagg agctgttata attgctgaat ctcatttgac
     1921 aatacataca tggcctgaat atggttatgc agcagcagat ttttttactt gtggtgatat
     1981 agacccatgg aaaagttttg aattattaga gcaactttta aagtctgaaa gaagcgagtc
     2041 tacagaaatt ccaagaggct taactacaaa aattaaaaaa tattcaaaaa aagatttagg
     2101 taagattact cataaaccag aagcagtata aaacatacaa gagaaagtga ggattattat
     2161 atggaacttt ggtatacaga agaatggaca gaaaatgtaa gattttctat aaaagtaaat
     2221 aaacatctat ttgaaggaaa gtctcaattt caaaggatag atgtgtttga tagtgatgaa
     2281 tttggaaaat ttctaacaat agatggatta atgatggtaa catataaaga tgaatttata
     2341 taccatgaga tgataactca tgttcctatg gctacaaatt taaacataaa gaaagtatta
     2401 gtcataggtg gaggtgatgg aggtacagta agagaattaa gccgttatcc tcaaatagaa
     2461 aagattgaca tggttgaaat agataaaatg gttgtagatg tatctaaaga gtatatggat
     2521 atatgctcat gtaaattaga tgataaaaga gtgagtttat attttgaaga tggtgttaat
     2581 tttgttaagt gtgcacatga taaatcatat gacctgataa tagtagattc aactgaccca
     2641 ataggaccag gtgaagggct tttctcaact gatttttata aggattgtta tagaatactg
     2701 actgatgatg gaattttagt aaatcaaagt gaaagtccgt attttgactt taatgctaaa
     2761 gagatgaaaa gagctaataa aaagttaaaa cagatatttc caatatcaga agtttatcaa
     2821 gcacatatac caacatatcc atctggtcac tggttgtttg gatttgcttc aaaaaaactt
     2881 aatccagtaa aaaatcaaga tagaaatgga tgggagaaat taagtttaaa aactaaatat
     2941 tataatagtg atatacattt aggttcgttt atgttacctc aatacgtgaa ggagatgctg
     3001 gatgaagaat aatttctatc atatgaacac ttttatgagt atgaacaaaa attatgaaga
     3061 atctaatctt atagtatttg gtgttggatt tgatggaact acttctaaca gacctggggc
     3121 tagatttgca agtagctcta tgagaaaaga attttacggt cttgagacat atagtccttt
     3181 tttagatttg gatttagagg attataatat atgtgattat ggagatttag aaattagtgt
     3241 tggaagtaca gaacaagtct taaaagaaat ctatcaagag acatataaga ttgttagaga
     3301 ttcaaaggta ccctttatga ttggaggaga gcatttagtt acattaccag cctttaaagc
     3361 agtacatgaa aagtataatg atatatatgt aattcatttt gatgcccata ctgatttgag
     3421 ggaagaatat aataatagta aaaattctca tgcaacagta attaaaagaa tatgggatat
     3481 tgtaggtgat aataaaatat ttcaatttgg tataaggtct gggacaaaag aagaatttaa
     3541 atttgctaca gaagaaaaac acacatacat ggaaatagga ggaatagata cgtttgaaaa
     3601 tatagttaat atgctaaatg gaaagaatat ttatctaact atagatttag atgtattgga
     3661 tgcatctgtg tttccaggaa caggtacacc agaacctggt ggtgtaaatt atagagagtt
     3721 tcaagagatt tttaagatta taaaaaactc taatataaat atagttggtt gtgacattgt
     3781 agagttaagc cctgattacg atacaacagg tgtatcgaca gttatagctt gtaaaatctt
     3841 aagagagtta tgcttaataa tatctgataa aattaaatag agtattttat ataaatagta
     3901 tatattataa tataagaagt tattttaagt ttgacgcttt tctaaagtaa cttcttattt
     3961 ttgtaattaa aaataaatat aataaacaac aaaaaagagc cctcgatagc tccttttcta
     4021 aaatatatat atttaaatta aataattata ttctatttac gttttcagct tgaggtcctc
     4081 tattaccttg agttatatca aagctaacct tttcgccttc ttctaaagtt ttgtatccat
     4141 cattttgtat agctgagaaa tgtacgaaaa catcatcttc tccctctaca gatatgaatc
     4201 caaatccttt ttcattgtta aaccatttta ctattccgtt tttcattata agaaaacctc
     4261 cgcattatta aaaaatacaa actaaaaaaa taagtctgtt ctattaatat aacataaatt
     4321 ttaaataaaa gcaaataaaa tttttaaaaa aattaattac ttttagaatt taataaaaat
     4381 tgttaaatta tacagtgaga
//
LOCUS       DAMJWU010000185         4373 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMJWU010000185 DAMJWU010000000
VERSION     DAMJWU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.190"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            152..1750
                     /locus_tag="PC460_003605"
     CDS             152..1750
                     /locus_tag="PC460_003605"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2602449.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1985..4066)
                     /gene="bclA1"
                     /locus_tag="PC460_003606"
     CDS             complement(1985..4066)
                     /gene="bclA1"
                     /locus_tag="PC460_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HDF2602450.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGVTGEIGPTGVTGPTGVTGSIGPTGATGPTGATGVTGEIGPTGATGPTGNT
                     GVTGATGPTGNTGVTGVTGATGPTGATGVTGEIGPTGATGPTGEIGPTGATGPTGATG
                     VTGEIGPTGVTGPTGEIGPTGATGPTGVTGSIGPTGATGVTGEIGPTGPTGVTGSIGP
                     TGATGPTGNTGVTGVTGPTGATGPTGVTGEIGPTGATGPTGVTGEIGPTGATGPTGPT
                     GNTGATGSIGPTGVTGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNN
                     GDAITGWQTIIGNSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFP
                     ILGGKDLAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATL
                     TIFRIADTVMT"
BASE COUNT         1278 a          785 c          679 g         1631 t
ORIGIN      
        1 tatattaaat ttgtagtatt tatgtaagaa ataaatttta tataatattt tttcactaaa
       61 tttttttctt tttacaacaa atactcacta ccttttgata taatattttt atcaattatt
      121 attatattca agttttgaaa ggagtattaa aatgagttta ttagttttcg gacataaaaa
      181 tccagataca gattcaatat gttctgcaat ttcattagca catttaaaga acaaactagg
      241 aataaaagca acagcttatg ctttaggaga tatcagaaaa gaagctaaat atgccttgga
      301 ttactttaag gtagatgcac ctgaaatttt agataatgtt agaatacaag ttagagattt
      361 aaattatgat aaagtagtac ctttaacacc aacttcatct atattagagg cttataacct
      421 tatggatgaa aaaaatgtta aaacactacc tgtaacttat gatgatggaa cttttgctgg
      481 tatcattact atgaaagaaa tagctaaaaa tctacttcat caacacttta caactataca
      541 cacatcacta tcaaacattt gtaaaaacct taatggtact attcttgtgg actgtggtga
      601 agatataaaa ggtagagtag tcactttatc atttggaatg gagactctaa tagagacttt
      661 aaaagaagga gacatagcta ttgttggaga tagatatgat agtattgaat atgctattga
      721 tacaaaagtt aaacttttaa tacttactaa tcatacagag atttcaaaag atttgctagc
      781 tcttgctaag ataaatggag tgtctgttgt ttctgttgaa agtgacaatt ataaaacttc
      841 taatgtaata aatcaatgta gtttcttaga ctctatagaa gctacagaaa atttaattac
      901 attcaaagaa gatgattata tggatgaaat aaaagagatt atgctagaaa caaacttcag
      961 atcttatcca atattagatg atgataataa gtttttaggt ttagtttcaa aaggtcatct
     1021 attaaaccca tctaaaaaga atgttgtttt agttgaccat aacgaatatg ctcaaagtgc
     1081 tgatggaatt gaacaagcaa acatagttga aataattgac catcataaac ttggcggtat
     1141 ttctactgat gttcctatgt catttagagt tatgcctgtt ggatgcaata gtactataat
     1201 ttatcaaatg tacaaagaaa ataatgttga gataccttat gaaatagctg ggcttctttt
     1261 atctgctata ttatcagata cattattatt taagtctcca acaacaactg atatggataa
     1321 gaaagcttgt gaagaattaa gtaaaatagc aaaagtagac atggaaaaat acgctatgga
     1381 tatgtttaaa tgcggtactt ctttagatga atatactata gaagaaatag taaatatgga
     1441 ctttaaagaa tttaatatga gtggacatag agttggtatt ggacaagtat ttaccttaga
     1501 catagactct atatttgcta aaaaagatga gtttttatct tatataaatt cgactgatta
     1561 tgataaatta attctttcag ttacagatat aatcaaagaa ggttcttatt taatctataa
     1621 agcagaagat agcttaattg ctaacgcatt tggtgttgaa ggtgttcaag gcacatttgc
     1681 accaggtgtt gtatcaagaa aaaaacaatt agtccctaat ttaactactg caatcaaaaa
     1741 ttttaaataa tataaaattt ttaaataacg aaaactgtct caaatctata tttgagacag
     1801 ttttttatta tatatatatt gataataaag ttaaataaaa atccataaat ttaaaatttt
     1861 tatgaatata ttttattatt attgttattt tattcattct aaaataggta aaactcaata
     1921 tataaaaata cttttgtcat ttaaataaaa cctaaatagt ttttaagatt attttagaca
     1981 cgttttaagt cataacagta tcagctattc taaatatcgt caaagtagcc gcagttcctg
     2041 ctgattgccc atttcgtatc cctactgttg tagatgaaaa gttatttatt gtaaaagtag
     2101 tcccaccaaa taaaaaacca gcaaaatatc cagaaagtac tcctccgcct cctggctcag
     2161 tagtaagccc tgccaaatct tttcctccta aaattgggaa taggatagcg aaagaatatt
     2221 gatttatact tgatgaacct ggttgtaatg ctactgaaac tgatatataa tacacaccat
     2281 tttcttgtac tgtaaacgta ccatttgcat ctactgttat acttgaacta tttcctatta
     2341 ttgtttgcca acctgttata gcatctccat tatttagtgt ttgtggagat ggtgcattta
     2401 cgaggaagtt agcaactggc tgagaggaat tgcctgttgc tcctgttgga cctgttgggc
     2461 ctgttactcc tgtcgctcct gttgctcctg ttggacctgt tactcccgtt ggccctatac
     2521 ttcctgtcgc tcctgtattt cctgttgggc ctgttggtcc tgttgctccc gttggtccta
     2581 tttctcctgt tactcctgtt gggcctgttg ctcccgttgg tcctatttct cctgtcactc
     2641 ctgttgggcc tgttgctccc gttgggcctg ttactcctgt cactcctgta tttcctgtcg
     2701 gtcctgttgc tcccgttggt cctatacttc ctgtcactcc tgttgggcct gttggtccta
     2761 tttctcctgt cactcctgtc gctcctgttg gtcctatact tcctgtcact cctgttggtc
     2821 ctgttgctcc tgttggtcct atttctcctg ttgggcctgt tactcctgtt ggccctattt
     2881 ctcctgtcac tcctgtcgct cctgttgggc ctgttgctcc cgttggtcct atttctcctg
     2941 ttgggcctgt tgctcctgtt ggccctattt ctcctgtcac tcctgtcgct cctgttggac
     3001 ctgttgctcc tgttactcct gttactcctg tatttcctgt tggacctgtt gctcctgtta
     3061 ctcctgtatt tcctgttgga cctgttgctc ccgttggtcc tatttctcct gtcactcctg
     3121 tcgctcctgt tggacctgtt gctcctgttg gtcctatact tcctgtcact cctgttgggc
     3181 ctgttactcc cgttgggcct atttctcctg tcactcctgt tgggcctgtt gctcccgttg
     3241 ggcctatact tcctgtcact cccgtatttc ctgtcggacc tgttactcct gttggtccta
     3301 tacttcctgt cgctcctgta cttccggttg ggcctgttac tcccgttggc cctatacttc
     3361 ctgttgctcc tgtatttccg gttgggcctg ttggtcctat gcttcctgtc gctcctgtac
     3421 ttccggttgg gcctgttact cctgttgggc ctgtacttcc tgtcgctcct cttggtccca
     3481 ttggacctgt aattctaatt ggaagacact cacatttagg ataacaatta tctataattc
     3541 tatcttcaaa tgttacaagt atatacggtt tgttacaacc tctactagat gtaaattcaa
     3601 gaatctgata agggtaattt aaagataaag ctaacccata attaggatat ttattcattg
     3661 cccatgcttt tatagttccc gtaatattta aacaaatata gttggatgta tcacttatcc
     3721 caattggtaa atatcccctg atattttcca tagacactct atcattccat gttatagttc
     3781 gtaaattata atattcactt attcttttaa tttcaatatc tactttttct ttaaaaatat
     3841 gattcttatt tgaatcaata tgaataaata actctgcttt taatatactt ttgtctttta
     3901 atatatctgg tattctcact ttaaaaaaag taatcaactt ttcttttaca aaactatttg
     3961 aacatttact tcctattaag caataatcta aattactaaa gtttttatct ggatattttt
     4021 tagatataaa agtatcatca tttaaataaa gtataatatt tctcatttat aatccctcca
     4081 ttttgggtct atatcatact atgtttaaaa tacatgctta gttacaatac aattaaaatt
     4141 tatagtatta aatttttata taataatgct gatttctaca aaaaaagaac atgtcattag
     4201 aaatttctaa tagcatgttc tttttcatta ttatatatta cactttaaat tattccttga
     4261 ctggcttagc tggtgtaaac taaaccagtc tttctaaaat attaagcact tttttcttat
     4321 tatgtttttt aggatttatt gtctatatta ttgtaaacca aagttataaa att
//
LOCUS       DAMJWU010000186         4340 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMJWU010000186 DAMJWU010000000
VERSION     DAMJWU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.191"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            231..1310
                     /locus_tag="PC460_003607"
     CDS             231..1310
                     /locus_tag="PC460_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2602451.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="PC460_003608"
     CDS             1634..2002
                     /locus_tag="PC460_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2602452.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDNDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="PC460_003609"
     CDS             2119..2922
                     /locus_tag="PC460_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2602453.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="PC460_003610"
     CDS             3307..4197
                     /locus_tag="PC460_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2602454.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVFKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1661 a          472 c          812 g         1395 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagaacgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tgctacctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tctactaact
      901 ggattgtaaa gcatggttgt agaactctac taaaaaaagg aaatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagct atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttagcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggataa tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtttttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaacaa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaattaca tactcattag
     2941 gtaaattatg tagtatatgt gtttatagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga agtattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggacagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagagat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggggta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaggcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgaggcagtg aaagaactta atgattctgg aattttttgt
     3841 ggtatattat tgactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgtatgta tggtgtaaca
     3961 atgcgtagtg gtcagcgaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtatttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tgttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gatattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtatta atattttagg taattgctag agctgatatt
     4321 atagatgaat attaatattg
//
LOCUS       DAMJWU010000187         4220 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMJWU010000187 DAMJWU010000000
VERSION     DAMJWU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.192"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            543..1451
                     /gene="trxB"
                     /locus_tag="PC460_003611"
     CDS             543..1451
                     /gene="trxB"
                     /locus_tag="PC460_003611"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2602455.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1541..3364)
                     /gene="typA"
                     /locus_tag="PC460_003612"
     CDS             complement(1541..3364)
                     /gene="typA"
                     /locus_tag="PC460_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF2602456.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3576..3818
                     /locus_tag="PC460_003613"
     CDS             3576..3818
                     /locus_tag="PC460_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602457.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3966..4157
                     /locus_tag="PC460_003614"
     CDS             3966..4157
                     /locus_tag="PC460_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF2602458.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1552 a          545 c          676 g         1447 t
ORIGIN      
        1 aaagtttaca aaactttaat ttgaaaaatg taattatatg gtatatcata taaatataag
       61 taaatagtgc taatatttaa actattaatc ataattggtt caacaaaagt atatagttaa
      121 taatgtttaa tttgaagaaa ttgttagtgt gaaattacat atatattcaa attaaattca
      181 taaatatttc acataataat agtatactgt aaacaaaagg aggtattaaa tatgggaata
      241 gtacttataa cagcagcatt agttttggtt atgtatggac tttcaattct atcatatata
      301 tcagaaagca atatattaga agacactgta aatgataacc aataaggttt tgaattatat
      361 aaataagtga gaactgtaaa taaaagttaa tttttatttg cagttttttt atatatatgt
      421 aaaataaagt taattaataa aaaataatgt tttgtttata gtatatatgg ataataatag
      481 taaagtataa gaatgatgag aataatatat aagaaataat aatataaaga gaggtaaacg
      541 ttatggtaga tatcattgta attggtgctg gaccagcagg tttaacttca gcaatttatg
      601 ctatgagagc agggttaagc gttacagtat ttgaaaaaaa tatttatgga ggtcaagttg
      661 caagtacctc tgaggtagaa aattatccag cagttcaaaa gatttctggg gtagaatttt
      721 ctaataatat atacaatcaa gcagttgctc aaggtgttga tattcagttt gatgaggttg
      781 aagagataaa tttagaggga aaagtaaagg ttgtaaaaac ttcttcaggt gaacataaag
      841 caaaggctgt tattcttgca aatggagttg aaagacgtaa attaggttgt gcaggggaac
      901 aagaatttac aggtagagga gtatcttact gtgcaacatg tgatggagcc ttttttaaag
      961 ataaagaagt tgctatagta ggtggaggaa atactgcact agaagatgct ttatttttag
     1021 ctaataattg tacaaaagtg tatttaattc acagaagaga tagttttaga ggtgaagaag
     1081 tgttggagaa atctgtgaaa gcaagagaga atattgagat actttacagt catggtgtag
     1141 aaaaaataga aggagaaaag actgtatcga aaatagaagt aaaaaactta aagactgaag
     1201 aaaaaagaac tatagatgtt agtggtattt ttattgctat aggtcttaaa cctaataata
     1261 aaatgtttga aaatgttctt gatttggatg aaggtggata tataatttca gatgagtctt
     1321 gtacaacatc agtagaaggt gtatatgtag caggagatag tagaactaag tttttacgtc
     1381 aaataataac agcagcatca gatggagcta ttgcagccgt tcaagcagct aattatataa
     1441 atgttgaata atataaattt caaataaaaa tagccatata aactgaaata atttttcagt
     1501 actatatggc tattttgtta attagaggta aatgtacgtt ttatttacct tgtctagatt
     1561 tgttatatct taatctatca tgttcattta agaatctttt tctaagtctt atagaatcag
     1621 gtgtaatttc aactaattca tcatcttcta taaactctag agcttcttca agagtaaata
     1681 ttctaggagg agataatttt atagcatcat ctgaaccaga agcacgtacg ttactcattt
     1741 tcttgttttt acaaggattg actaccatat catcttttct ggaattcata ccaattatca
     1801 taccttcgta aacttctaca ccagggtcta caaacattac agctctatca cttaaggcat
     1861 taagagaata ccccatagta acaccaggtc cttgtgctat taaaacacca tttcctcttg
     1921 aaggtatttc tcctttaaat tcttcaaatt tttcaaatga acgaactaaa gtaccttctc
     1981 cacgagtagc gtttataaat tcacttcgat atcctaaaag accacgagta ggtgctaaga
     2041 actctatttt tacatagtct ccttctattg acatagactc catcatacct tttctcatgt
     2101 ttaattcatt tataatagta ccagagtaaa cttcaggaca gtttacaaca actctttcta
     2161 ttggttccat tagttttcca tcttctttat gcattaaaac ttcaggttta gaaacaccaa
     2221 cttcataacc ttcacgtctc atattttcaa gtagtataga taagtgaagc tctccacgtc
     2281 cagaaacttt gtatccatca gtagtatcaa gaggttcaac tttaagacca acatttactt
     2341 caagttcttt ttcaagtcta tctttcaaat gtctagttgt aacgaatttt ccactttttc
     2401 caacgaatgg agagtcgttt actaagaagt tcatagaaag agtaggctct tctattttta
     2461 tcatttccat aggtagtgga ctatctaaat cgcatatagt ttcacctata gatatatcag
     2521 gaataccagc tataacaact atttctccac tagtagcttc atcaacttca acttgtttta
     2581 aaccttcata cacagaaagt tttgatactt ttcctttaga tacaacagaa tcttctctac
     2641 atatagcaac ttgtgtattg tttttaagag tacctttgta aattctacct ataccaagtc
     2701 tacctacata atcatcatat gcaagagctg atatttggaa ctgtagaggt tcattatcgt
     2761 aattaggata agcttcaacg tgatttacaa tagttttaaa tagaggagaa aggtcttcac
     2821 tatcatcatc catttcaagt ttagcaatac cttgtttagc aataccatat attataggaa
     2881 attcgcattg ctcatctgtt gcatttaagt ctacaaataa atcgaaaact tcattaacta
     2941 cttcttccgc tctctggtct tttttatcaa tcttgtttat aaataatatt ggttttaaac
     3001 cgaactctaa agatttttgt aaaacgaatc tagtttgagg cattgggcct tcactagcat
     3061 caactaatag aataactgtg tcaacagttt tcataacacg ttcaacttca gaagaaaagt
     3121 cactgtgtcc tggagtatca actatgttta ttttgtaatc ttcatagttt attgcacagt
     3181 tttttgaata tatagttatg cctctttctt tttcaaggtc gttactgtcc ataacgcagt
     3241 ctttaactac ttcgttcttt ctaaaaacac cgctttgaga aaggaaagca tcaaccaatg
     3301 ttgattttcc agcgtcaacg tgggctataa ctgcgatatt tataatttta tgtttttgtg
     3361 acatttttgt tgctcccttc gattaaaata acttattaat tatagcacaa tatattatta
     3421 ggtacaagga ggaatatatt aaattgtatt aatatagttt aaattggata aaactgttag
     3481 ttttgcatct gaaaattatt ctgtttaaaa aaatttttta gacaaataat aaccttaata
     3541 gataaaaaga ttaataaaag agtaaaggat gatatatgtt ggaagtaacc aaaataataa
     3601 ttaaatattt tgcaagtgaa agtgatactt ttgtaaaaga agaagtttgt aattttgaag
     3661 aacttcttaa gtggtctcat aaatttattg aaggtaaaaa gatagaagat gttttgtata
     3721 taaagtctaa agaaaactat aaagtaaatg tgattaagtc tttagaaatt aaggaattgg
     3781 aaatagtgag aaaacaacag ataccaccgt acatgtagta ttaataatat ataaaattaa
     3841 tatgtaatac tcgcaatagg atataaatgg tatatatggt aataatttat attaaaaaaa
     3901 cttttataaa gtttatattt gggtataaaa gtattaatta gacattaatt aggaggaact
     3961 taaatatgat aagagtatgt ccaaactgtt caaatgtgga tgtaaataag ctaaaaactt
     4021 tggttggaga agaaaatgta aagactggtt gtataggtca gtgtagagca tttaaaaaag
     4081 aagctgttgg aataatagac aatgagcttg aaataaagga aacagaagca gagttttttg
     4141 aagcatgtaa agaataaatt ttaaataaaa tataaattat aataaataga gcatctaaat
     4201 tgtataagca ttaaacaatt
//
LOCUS       DAMJWU010000188         4120 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMJWU010000188 DAMJWU010000000
VERSION     DAMJWU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.193"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(128..1045)
                     /locus_tag="PC460_003615"
     CDS             complement(128..1045)
                     /locus_tag="PC460_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF2602459.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            1307..1684
                     /locus_tag="PC460_003616"
     CDS             1307..1684
                     /locus_tag="PC460_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2602460.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1840..2289)
                     /locus_tag="PC460_003617"
     CDS             complement(1840..2289)
                     /locus_tag="PC460_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2602461.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2456..2860)
                     /locus_tag="PC460_003618"
     CDS             complement(2456..2860)
                     /locus_tag="PC460_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF2602462.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(2973..3839)
                     /locus_tag="PC460_003619"
     CDS             complement(2973..3839)
                     /locus_tag="PC460_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2602463.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRIKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
BASE COUNT         1352 a          683 c          450 g         1635 t
ORIGIN      
        1 acttttaaat attgaaatat ttctttatat atattattgt atatttctta taaattattg
       61 tcaatttatt ttttcttaaa aataaaataa ttgcataatt tatttcaaat tatgcaattt
      121 ataaaattta ttcccaaaac tcttttactg cttcgattat ctcactataa ggcaataaag
      181 tcgcttcgat tattgattta tttgtctttt ctaaataata cttaatcata taatatccac
      241 aagcatatcc tgcacaataa ggcaacccta ctggaaaata tccttgcatt ttagctatat
      301 catcaccata aagataagat gttatatttt gaaatccagt ttcttttaaa gcacttttta
      361 ttattggctt tatataagta tttaatgttt cgatatctgt tctactgacc caaggtccta
      421 acatttcctc tccaaacatc catgttgcaa aattttctgc aagaccttca tttatcatca
      481 tttcttctaa tgttatatta ttattccact ctataaactg aaatctaata ttgtgattac
      541 attcatgtgc tattaatact ggtaatctat tgatagtata ttcattagga accaatgata
      601 gaaatatata tccaggaatc ccaccatcac cccagtatcc atcacttaat attgtataag
      661 gactatttgg attcgccaat aatattgaat atttatagtc cttaatattt aaatcatacc
      721 cttcttttat aaaagcattt atagagtttt ctatggtttc tttacaagtg gcccaaattt
      781 tatcagcaga taacacattt atattctttt tttgtttttc atcaatatcc ttaggcgata
      841 aaacccctaa catttcactt gctataatta catcatatcc acctttctgt ctagcaacta
      901 ttggaacatt catacacttc cacttaaatt caaaaggctt catcaattca tatctgtata
      961 tatcttcttt tttgtttatt ggtgcatcca ttatcttctt gtatatttta tctgagcgaa
     1021 tttctataat attcatattt accatctcct ttaagacaaa gtatatacaa ttacgtagcg
     1081 tcaatgtcaa tatacatttt ttataatgtc accttaatct ttttattata ttttatcctg
     1141 taaaccttcc gtacatataa cattttttta aattaataaa tttgtataat ataataataa
     1201 aaaattatat acttcaaggt ttccctatga tacaatttac taaatcataa tttagattat
     1261 caggtgcaac tataaattat tctatatcaa ggaggatata ttggtcatgc taactccata
     1321 tttaatattt aatggtactt gtgaaaaagc atttaatttt tatgctgagg ctttcggagg
     1381 aggaaaaact atatttgcgc gattagacag caatccaaac aatcctatta tgcacgcaag
     1441 tgttactttc acaaaatacg aaggttgtat aatgggtgcg gatacagaca agcctgttgt
     1501 aatttctggc atggcgattt gtgttgttct accatctcga gaagcgatag aagaaatatc
     1561 tgtaaaactt gccgaaggtg gtacacttgt acaagaattt ttaccacacc caccaccaca
     1621 tcaaaatgat ggcgctgctg aagtacttga taggtatggg tatacttggt atttaagtac
     1681 atagttaaaa taaaaaaacg acatctaata atcataatat agaacagata aatttcaatt
     1741 taatagtgta gtaaaaaagt cgtgtcctaa gactgtcacg acttttattt gtataaaaat
     1801 ttaagtaaat atttttgcga ataaatgaga agtataactt taaattaatt tcatatgtag
     1861 tattggaaat gggttacctt gtccatctat ttcagaccta tcaaacactt taaatcctaa
     1921 gtgaatataa aatcctacag cttttttatt ctgctcattt acatcaacat attttacatt
     1981 gtatttatct atagcatatt ttattaattt tttaccaata ccatttcctc tatatttatc
     2041 ttctataaag agcatctcaa ttttatcttt atctattcct ataaagccct ttattatatc
     2101 attatctttt atacaaacta tattttctac ttctttaagt ccaatctcaa ctaatggtat
     2161 caaagattca atatcttttt ctgttaaaaa atcatgcgtt gctcttactg atgattccca
     2221 gacttgcaac atttcgtcat attctgttga atttatttgt ttcatttcaa gaattattcc
     2281 agatttcata aaaatttcct ccctatatca cttttaattg cataacactt ttttatacaa
     2341 aacttagaaa ggactacgaa ggttaaatat catagtcctc attaagttta aatattatct
     2401 attatatatt attatactat aatctagcaa attcactaca ataattcaca tcagcttata
     2461 ctaaaggtgc ttctatttga gtagtaaaaa ggaatttatg acaatgacca tcattaacag
     2521 tagtttctcc acatattaag tgaatgtgtt tatcagttcc aggtattcta attgctggcc
     2581 ctgttgtaac acatatctta tggaaatggt caaggaaatc tacattatca tttattttat
     2641 gaacatgatt tgtacctcca tttattggta tagcttcacc agtaacccct gctgcacggt
     2701 ggttatgtct atcctcacat tcttctgcta acttaacact ttctgaatat tcatgaacat
     2761 gtgtcatttc ctcataatca ttatagcact tgttgcactt attgtattta taatcatcat
     2821 acacataatc tctttcacat ttataatttt gataactcat ttttatatct ctccctatat
     2881 tatattaaga aattcttgta aagaatctct aatgtataat atgaattaaa gtcaaaaaag
     2941 ttcatgtttt gtaaaattat gtattaattt aattattttt cccagtattt agggcgtatt
     3001 agtgaagctg gtaacttcgt actagaatct ccttttgcag tatttacttg taattgtgtt
     3061 aaaaatatac tattttcaag attagcacct ctaatatcac aatctcttaa gtctgctcct
     3121 attaaatttg ccccactcaa atctgtattt cttaaatttg ctgctattag aaatctacct
     3181 cttaaatctg ctcctattag atttattttt ctaaggtctg ctcctattaa atctatattt
     3241 ttacttaatt ttttcatgtt tttaagagta gaagtcttga taaatgattt aacctttccc
     3301 atcacagact cactcgcttg tgataataat aaattaacct ttattctatg tgatatgata
     3361 tccaaattaa ttatctgttc tgggctcata ttagttatct tttcagtttc ttcaattatt
     3421 aaatcaattt ttgatttgat tgtatctgat atatttaata cagatgcttc tttaagatac
     3481 cataacattt catgtaattg tctcataatt aaaaacactt cagacatttg atttgttagt
     3541 tctgatgatt gcatccagtc tatcccctta tatgttactt gtgctacttt ttgacctgaa
     3601 ccaaaacaat catatgcagt acacccttta aaacctcgct tcataagact attatgtaca
     3661 gaacatttaa aatctggttg taaattaata cagggttttc ctgattcctt atcgatagga
     3721 aatccatctg aagcagaaaa ataaagagct acacagcaaa gtccaaagca cttcttacag
     3781 tcaatcttta gctcctcaaa taacttatta tcatatatat ttttatgtat atctatcata
     3841 ttttttctcc tcttaaactt tatttttaat attatattat ctataactaa atgataatca
     3901 agtcccttat ttaacagttc ttattcttta ttataattta aggtattatt tattccaata
     3961 gcatacataa gtttctttat acttgtaaca atacctttat actaagactt attttaaatg
     4021 tagcactgta ataataaatt gtttatatac tcataatgta tttgataaat tgtaaatctt
     4081 tatagaagtt gaaattttta atgattttag aatcaagata
//
LOCUS       DAMJWU010000189         4043 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMJWU010000189 DAMJWU010000000
VERSION     DAMJWU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.194"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            24..422
                     /locus_tag="PC460_003620"
     CDS             24..422
                     /locus_tag="PC460_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2602464.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNIK
                     INLNIKGYKFLPYVKNLQASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            410..1348
                     /locus_tag="PC460_003621"
     CDS             410..1348
                     /locus_tag="PC460_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2602465.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1732..1956)
                     /gene="secG"
                     /locus_tag="PC460_003622"
     CDS             complement(1732..1956)
                     /gene="secG"
                     /locus_tag="PC460_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF2602466.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2549..3841)
                     /gene="eno"
                     /locus_tag="PC460_003623"
     CDS             complement(2549..3841)
                     /gene="eno"
                     /locus_tag="PC460_003623"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF2602467.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1385 a          646 c          459 g         1553 t
ORIGIN      
        1 atataaaatc ttaaggagtt gccatgataa aaaaaataga acatgtggtc attactaata
       61 ataatataga aaattatatc aatttttata aaaaatttgg tttttcatct aaagaaatga
      121 gcaaaaatta taaacttaat gctagaaata ttaagataaa tcttaacata aaaggttata
      181 aatttttacc ttatgttaaa aatttacaag caagtagaga taacttatgt tttaaaataa
      241 acacaaatat tgatgagcca aaaaaatact tagaacaaaa tgagattcaa attaagcgag
      301 gaattgtaga atgtacaggt gtctttggta atatgaaatc tatttatttg aaaaaaacta
      361 atggaaattt aattgaatta tcaaatcata gaatcacgga gagtaaacct tggaaaattt
      421 aatcctatca ataaatgttg ttcttccttt gtttctcaca atgtcacttg gatatgttct
      481 aaaaaaatta ggtctatttg atactcatac tttaaataaa atgaataatg tctgcttcaa
      541 gtctttcttg ccattattat tattttttaa tatatacaat acagatttag atggttctct
      601 taattttagg cttatggctg tagctgttat aattattttt tcaatcttct taatcttagt
      661 ctttattatt cctaaaattg aaaaagataa taagacaagg ggtgcattaa tacaaggtgt
      721 atttcgtagc aatttcgtta tatttggtat tcctgtgaca atttcactat ttggcgataa
      781 gagtattgga gtcacatctt tattaattgc agttattgta cctatgttta atgtattatc
      841 tgttgtaatt cttgaaattt ttagaggtgg caaagtaaat ataaaacata tgattaaaag
      901 cgtcttaaca aatccactca taatcgcctc ttttatagga ttgacaatgc taattttaaa
      961 tattaatata cctacatttt tacacaaaac tattggtgat atttctaaaa tagctactcc
     1021 tttatcactt ataattctag gtggctcttt tagtttttct gctataaagg ggcatataaa
     1081 ccccattata ttgggcgtat gtggaaaact tatacttgta cctgctatat ttttaccaat
     1141 tagcattttt cttggattta gaaatgtaga attagcttca cttatgataa tgttatctgc
     1201 tccaactgca gtttcttcat ttacaatggc acaacagatg gattctgaca gtgaattagc
     1261 tggacaaatt gtagtattta cttctggatt ttctataatt acagtatttt taataatatt
     1321 tattttaaag cagaataatc ttatttaatg atatttgata acacaaattg gtattatgag
     1381 aaacatatat aaaatcgatt taattgcgta aaaacatatt attatactat ttttactata
     1441 taatataact ttgtgtatat tatctcatta aacatggtga aagtagtgtt gctatatatt
     1501 ttttagtttg aaaaaaatat ataaatacaa acaatatata aatacaaata ataaacgcca
     1561 acaaatagtc aatctataaa ataaaaactg gactaatcat ataaaatcat tagtccagct
     1621 tttattttac gtacttttta tgttgatgta cttttattaa aaactaaatt taatttttaa
     1681 atgtctagca aatgcactaa ttaaataatg gccagttatt caacaatatc actacttctt
     1741 aactataagt aatgccaatg catttataaa aaataatact gctgttatct ttgtaactct
     1801 agcaagaaat gcttgttttc cttttggttt aaagtaagct tggcttcctt ctccaccaaa
     1861 ttctgaaggt aaagcattct tagaatcttg aggcattatg ctaattacta gtaaaatccc
     1921 tagtactact tgcacaccca ttaaaatgtt gctcattctt gcccctccta tctgtttatt
     1981 acttaaattt cattactctt tagtgtatca caataatata gccatttcaa gactattatg
     2041 ctataaataa catacaaaaa aatatattgt tactataata tattactata ttctcaatat
     2101 aaaaatgaat aaaacttaga agagaaaact atttaaatta atgcataaat taacacttaa
     2161 aataatctac tctcattgta tatattataa atagccacca aaataaaaca atcattttaa
     2221 aatattgata taacttttaa tttcaacata atttaaaata aataatccac atttagcaat
     2281 aatactttaa tgacaattat gattaaatca aaatatgtct tcacttttat tttttaataa
     2341 aaacctccta tgcaattaga taaaaatgag atagaaacat ttattcatat tttttctgaa
     2401 tctatgaata aaattaagta ataaaatata aaaaaggata agtgaattga aaaatcaatt
     2461 aacacttatc tcttttatat cttataagat gagcttaaat atcttatatt ctctcttcat
     2521 taatcttttt tagtaaccat tttaataact atttttttaa gttatagaaa gattttaatc
     2581 cacaatatct agcttgctct ccaaccattt cttctattct taataattgg ttgtacttag
     2641 caactctatc agttcttgat ggagcaccag tttttatttg accagcattt actgcaacag
     2701 ctaaatctgc tatagtagaa tcttcagttt ctcctgatct gtgagatata acagcagtat
     2761 atcctgctct cttagccatt tctatagcat ctaaagtttc agttattgta cctatttgat
     2821 taactttaac taatatagag ttagctactc cattttcaat tcctttttct aatctctctg
     2881 tgttagtaac aaataagtcg tcaccaacta attgtaattt atttcctaat ttttcagtta
     2941 ataatttcca tccatcccaa tcttcttcgt ctaaaccatc ttctatagtt attattggga
     3001 agttgtttga ccaatcttca tataaagcaa ccatttcagc agcagttaac tcttttcctt
     3061 ctcctgctaa aacgtatttt ttagtttctt tgttgtacat ttctgtagca gcaacgtcaa
     3121 gacctaacat aacgtcttca cctggcttat atccagcttt tgttatagct tcaactatta
     3181 attctaaagc ttctctgttt gaacctaagt ttggagcaaa tccaccttcg tcacctacac
     3241 cacaagctaa tcctttttct cctaaaactt tctttaacga atggaatact tctgctccca
     3301 ttcttaaacc ttctttgaaa caacaagctc ctactggtaa tatcataaat tcttgaacat
     3361 caacgttgtt atcagcatgc tctccaccgt ttaatatgtt catcattgga acaggtaatt
     3421 gtttagcatt tactccacct aggtattgga ataatggtaa acctatttca tcagcagctg
     3481 ctctagctac tgccattgaa acacctaata tagcattagc tcctaattta cctttgtttg
     3541 gagttccatc taattctatc atgattgcat ctatagctgg ttggtcaaat gcatccattc
     3601 cttctatttc aggtgctatt atttcattta cgttagcaac agctgtttct acaccttttc
     3661 ctaagtatct tcccttatct ccatctctta attcaacagc ttcaaaagct cctgtagatg
     3721 ctcctgatgg aactattgct cttcccattg ctccatcttc taatactact tcaacttcta
     3781 cagttggatt tcctcttgag tctaatactt ctctagcata tactaattca ataactgaca
     3841 tttgtatatc tcccttcgat tttctattag ttaaattttc atttactgta aacatccgtc
     3901 tactatttta atctactttc tatattatag tacataaagt tgcttttgaa aattaggtac
     3961 aaatttattt gatgggtaaa tttgtaaaag ttcctatttt tattttattt tttataatat
     4021 tttaatttat ataatataaa aaa
//
LOCUS       DAMJWU010000190         4027 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMJWU010000190 DAMJWU010000000
VERSION     DAMJWU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.195"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..727
                     /locus_tag="PC460_003624"
     CDS             <1..727
                     /locus_tag="PC460_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2602468.1"
                     /translation="KLGMVKIRDKQVPQGRILNATISKEPSGRYYVSLCCTDVDIEAF
                     ENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKA
                     RLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNMIKNHKLSRLISDVSW
                     SEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETH
                     DRDINASINILKEGLRLITIQNK"
     gene            854..2023
                     /locus_tag="PC460_003625"
     CDS             854..2023
                     /locus_tag="PC460_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF2602469.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(2186..2728)
                     /locus_tag="PC460_003626"
     CDS             complement(2186..2728)
                     /locus_tag="PC460_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF2602470.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2876..3160)
                     /locus_tag="PC460_003627"
     CDS             complement(2876..3160)
                     /locus_tag="PC460_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2602471.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(3223..3987)
                     /locus_tag="PC460_003628"
     CDS             complement(3223..3987)
                     /locus_tag="PC460_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2602472.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         1418 a          535 c          623 g         1451 t
ORIGIN      
        1 taaacttggt atggttaaga taagggataa acaagtacct caaggtagaa tacttaatgc
       61 tactatatct aaagagccaa gtggtagata ttatgtatca ttatgctgta ctgatgtaga
      121 tattgaagca tttgaaaata ctaataatca gataggttta gatttaggaa ttaaagaatt
      181 ttgtatctca agttgtggag aatttataga aaatcctaag tatcttaaga agtcattaaa
      241 caaacttgct aaattacaaa gagaattatc gagaaaaaca atcggtagtt taaatagaaa
      301 taaagcaaga ttaaaagttg caagacttca agaacatata gctaatcaaa gaaaggattt
      361 tctacaaaaa ctatctacta aattaataaa agagaatgat atcatttgca tagaagattt
      421 acaagtaaag aatatgatta aaaatcataa actatctcgc ttaatttcag atgtatcatg
      481 gtcagaattt attcgtcaat tagaatacaa ggctaattgg tatggtagac aaattgtaaa
      541 ggtaggtaaa ttctttgcaa gttcacaaat atgcaataaa tgtgggtata aaaacgagga
      601 agttaagaac ttaaatataa gagaatggat ttgtcctagt tgtaatgaaa ctcacgatag
      661 agatataaat gcaagtataa atatattaaa agaaggatta agactaataa caattcaaaa
      721 taaataacgt atgtaagaac cggaggaact acggggatag cctgtggaga attagtaaga
      781 catattttag tatgcaaaat tctgtgaagc aggaaccctg tgactttagt catgggaggt
      841 tcagggtaga aagataaatg aactttgtaa agatgaagtt gtagagtttt gcaatagaat
      901 aaaaggtaaa gtggaaagtg ttgcaataag tggagtattt gctccagtaa ataaacaaca
      961 agaagaagaa gttgcaaaat gggttagaga aattttaggg gatattccta tatctttatc
     1021 aaatgaaata ggtaatatag gtatattaga aagagaaaat ggagctattt taaattctgc
     1081 attgtctcaa gtaggtaaag ctgtatcttt aggatttgaa aaagcactta aagacttagg
     1141 tatagatgct caaatatatt tcagtcaaaa tgatggaaca ttaatgaatt tagagtatac
     1201 aatgaaatac ccaattttta caattggatg tggaataaca aattctataa gaggagcttc
     1261 cttcttatca aaagtaaaaa atgcaatagt attagatgtt ggtggaacta caagcgactt
     1321 gggtgtacta tataatggat tccctagaga atcatctatt ccagttacaa ttggaggagt
     1381 acatacaaac tttagaatgc cagatgtttt aactattggt cttgctggtg gtacatgtgt
     1441 aaaaggcgaa aaagataata taaagatagg tcctgaaagt gtgggatata gaataactga
     1501 agatgcaata atatttggtg gagacactct aactttatca gatgttgtta caaaattaga
     1561 tatggcgttt gaagatagaa aaagcaatgt tgaaaactta gataaagagt attgtaatga
     1621 ggtatataag aaggttattt taaaattaga agatgctgta gaccaaatga agactagtca
     1681 agatgatgtt gaattggttt taactggtgg aggaagtata attgttcctg aaaaactaaa
     1741 aggcataagc aatgttataa gacctcctca ttatactgct gcaaatgcta ttggtgcagc
     1801 tttaggacaa ataagtggag atgtagaaaa agtatacagt ttagataaaa tgtcgagaga
     1861 agatgccata gaagatgcaa aaaaagaggc tgtaaataaa gctctaaaag ctggtgctaa
     1921 agaagatact atagaagtat taaatataga agatgttcca cttgcatacc ttcctggtaa
     1981 tgcacttctt ataaaagtaa aagttgttgg tgatttaatt taaagaataa atataagtat
     2041 ttccagaatt atataaatat ttctggactc atttttaaac ttattttatc aataaaaaca
     2101 gcataacttt tgactatatt cttataagtt tccgaacagc cgatatatac ctattttttg
     2161 aggtttatcg gctgtttgtt atcttttatc gcattccaac tatttttctt accgtatttg
     2221 ctgtacgaat tgtaatctta tcacttacat ttgaactggc agtctttgac caccaatttg
     2281 tctttttata atctttacga ctaaaagacc aaaagatagc atttttataa tgatatactt
     2341 tttcatactc ttctttagga tttccaacct catcataaaa ctcgtcataa gataagtcag
     2401 gaaacataaa aataagatta tcatatattt ctttgttgtt atctccccac caattaggag
     2461 cattatttag tatatctttt aactcttctt gtgaaataat aaaaactggt atttccaatt
     2521 caaactgttt ttttatcatt aatcttattg tatttgagat aaccttgctg tcatctacat
     2581 cacttaaaaa gacaacatta ccactattaa ggtatgtaga aacttctgaa taaccaagtt
     2641 ctacaaagct tgcttttaat tcagacataa ttatcttgtt cttaccactt atattgatac
     2701 ctcttaataa tgcgatataa cttttcatat aataatttca cctctaaaca ttcacatttt
     2761 tctatgtagt acaaactgga atttgcatct tttataattc tttcaaaact tcttcatatg
     2821 taattttttt aatataaaaa aatcggtgct taatttgtac cgatttttgt ttttcttagt
     2881 aaagaactcg tttttttgct tctgctatag ctctagcagc actttttgct tgcattcttt
     2941 tttcttcttc ttttttaaat tcatttattg tttcatttag attcttatca tgttcatttt
     3001 tttctttatc ttcgttttct ctgtatacta atatataatc tattattgat tcgtttatta
     3061 tttgagatat acttttattt tcattaattc ctattctctt aacttcctta agtagagtct
     3121 catcaatatg aaccgttgtt tgctttgtat tttttgccac cttaatcacc tcttaaaaaa
     3181 aattctaaca ttaaaatttt ttttagtcaa taaattttaa tattaaaatt ctttaaatgt
     3241 tttatttgat tttctaaaat tttctattgt atttatcatc ttatttttta tattttcacc
     3301 tagtacagaa gactttagtc taggattttc gatatggtca taaattggtt ctaaaaattc
     3361 gacttcgaca ggaacagagt taattttacc aataggttgg tatccttcat aagtatcttt
     3421 tgaatttttt attactagtg gaacaatagg gcattttgct ttatatgcta tctttaaagc
     3481 tccacttcta aactccgaaa ccaatgaatt tggttcttta atccatgtta aatctccttc
     3541 tggaaacact gccatacttt gacctgtaag tatattttga gaggcttggg ctatactctt
     3601 tataccctca cgattgtttt ttctgtttac atatacacat cttagtaatt ttgcccattc
     3661 tttaaatata ggtatatttc tccaaactgg ttcatctgca attacacatc caattggtct
     3721 ttctacactt gctgttagta tgaagctgtc aagcatacta gaatgattta caacaaatag
     3781 aagtggttct tttggcagtg tttccttacc aattatatta agctctatgt tcactatatc
     3841 taaagatttt ttagcttgtt tttgcataaa ttcaaatttt tcctttaagc taaatttatc
     3901 aggattcttt ttaattttaa taagcctagg tatagaagat gtaaaaccta taagttgata
     3961 tgtagcaaat tttaagtaat tattcataaa attatctcct taaaataaaa ttatttatta
     4021 ctgtaaa
//
LOCUS       DAMJWU010000191         4025 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMJWU010000191 DAMJWU010000000
VERSION     DAMJWU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.196"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(66..926)
                     /locus_tag="PC460_003629"
     CDS             complement(66..926)
                     /locus_tag="PC460_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2602473.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1075..1965
                     /locus_tag="PC460_003630"
     CDS             1075..1965
                     /locus_tag="PC460_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2602474.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            2183..3937
                     /locus_tag="PC460_003631"
     CDS             2183..3937
                     /locus_tag="PC460_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF2602475.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1643 a          422 c          627 g         1333 t
ORIGIN      
        1 gtataatata acatataaaa agttgaattt gtatatgtta attatacttg ctatttattt
       61 gttagttatt ctattttttt aaatccatta cctaaagctt catgtatatc tgtaattata
      121 ataaaagcat tagggtcgac attttttact aattttttca attcgacaac ttctttcttt
      181 tcaactacta ctaaaagtac atttttattt tctccagtat atgcaccttc tccttttaga
      241 atagttgcac ctctatctaa atcttccatt atttttttac ttatatcttt tgaaaggtca
      301 gatataatag tgaatgattt ggaataatta aatccttcaa taagcaagtc actaactttt
      361 actaaaatgt ataaagccac agttgaataa agtgcaattt caacctgtct atttactata
      421 cctgataaaa caactacaac gcagtctaca atacccatta aaacaggtat actaaatgta
      481 ggaaaatatt tatttagtag gagtgccatt aaatcagttc cacctgtaga gccatttatt
      541 ctaaaaataa gtccttgacc aactcccatt aaaatagaac cagatataat tgcaagatac
      601 atatcatttg ttatatctaa tgtagccata tttgcagtaa gcttaagtgc tatggtaaga
      661 aaaattattc ctaaaaccgt tttaaaacaa tctttttttg aaagtatttt gtaagcaaat
      721 atgaataatg gaacatttaa aagtaaattg acaatccaaa gttctacacc gaataatgaa
      781 tttaaaacaa ctccaagtcc tgttatacca ccagcagcga ttgcatgtgg attaaaaaat
      841 aagttaagag aaatggccat taatatacag cctacaatta atattaaaat ttctgaaaaa
      901 gttattctat cttttttcaa taacatcact ccttatatat agattataat atatattata
      961 tagtttttcc aatttttaat aaaagaaata aaaatatatt ataaaaacaa tttgattgga
     1021 attttcagaa aattaatgct ataattaaaa gtaaatttaa agggaggtta taaaatggct
     1081 aaattaactc tagaacatat aaaaggaaat acatattata ttccactccc tacaatagta
     1141 ggtgtatacg tagatggtaa ggatgcaatt ttaattgaca gtggaaataa taaggataca
     1201 gctagacaag ttttaagatt attagaagaa cataatttaa atcctaaatt aataataaac
     1261 actcattcaa atgcagacca tataggagga aatgcatatt tgaagaatca aacaaaatgc
     1321 aagattgcta cgactaaaat tgaaggatat tttacagaaa atccaatatt agaatcagca
     1381 tttttatatg gtggctatcc aagtaaagca ctgaagaata aatttttatt agcaaaggaa
     1441 agtgaagttg attatattat accttcaaat ggcaaaattg tagatacaga acttgaagcg
     1501 atttctcttc caggtcatta ttttgaaatg attggtgtta gaactccaga taatgttttg
     1561 tttgttgcag atagtcttac accagagaat ataattacta aatatcattt tttcttccta
     1621 ctggatatag attcacagtt taagacacta gaaaaattga gaatgttaga ggctgatttc
     1681 tttgttccaa gtcactctgt caaaactaca gatataaaaa gtttaattaa tattaataag
     1741 aagaagatgg aagaaataat agataatatt aaaaaggttt gttatgagcc agttatgatt
     1801 gatgaagtta ttgaaaaaat gtgtgatttg tacaatgtca agcttgatgc taatcaatat
     1861 gtgttggttg gaagtacaat acgttcttat ataacttatt tatatgagaa taatatggtt
     1921 gaatatatat ttgatggtgg taagatgatg ataaaagctg cgtaaatagt aataattttc
     1981 aaattatatg atgaaattgt tttagtatga ctgttatttg aaaagtaata taaaaaaatt
     2041 tatataaata agctaagatg attgatataa agttactgat aattttaagg taatatatac
     2101 gttactataa tattatgtta ttatttgtaa ataaggggat aatttataag caaattaaag
     2161 accttaattt acaggaggaa tagtgtatat tatgaataaa aaattcaaaa aattcagtaa
     2221 aaaactaagt atattaattt tagttttgac tatgtttctt tttaataatt taactgcaaa
     2281 tgcacagatt aaagaagatt caataatagg aattgataga tatgaaacag caggacttat
     2341 agcggataga caaacttatg atactgtaat attagttaat ggagataaaa atttatctga
     2401 tgggctttca tcaagtggat tagctggagc tattaacgca ccaatacttt tgactaaaaa
     2461 aagtgaaata ccaaaagcta ctttaaatag actagataat aagagtttaa atgttgtaaa
     2521 aaaagtatat ataattggtg gttataatac tattgaaaat tcagtagaaa aagatataaa
     2581 aagaaaaggt attcaagtgg aaagaataaa tggtaacaat agaattgaaa ctagttataa
     2641 tgtagctaaa aaaataaatg aagtaagtag tgtaaaagag gtaatgctta caaatggatt
     2701 tgtaggagag gctgatgcta tgagtatagc acctgtagtt gcaaaaaata aaggagctat
     2761 aatacttaca gatgggaaat cagttccatt taagactgaa ggtttaaatg tttacgctat
     2821 aggtggcaaa tctgcgataa atgaggattt agtaaatgaa actaaagcaa ttagaatcgg
     2881 aggaaatgat agatttgaga ccaataaaaa ggtaatggaa aagttttata atggagctac
     2941 agatttttat ataacaaatg gatatcaatt agtagatgca ctaacattat ctcctttagc
     3001 caaagaaaaa ccaatcgttt tagttgctga tggaagtaat aaaggtattt taaaaggagc
     3061 taaaagtata acaaaagttg gaggtattga tgccaatata tataaacagt gtttagatat
     3121 gattaaatat aataatatgg atatgactcc aaatatttta aaacacttaa cttcagttga
     3181 aggaaatgaa gtttctgaaa tacttataga atcagataat ttaggtgtgg ttgttgaaaa
     3241 gacgaatacc aataaatttg agtttgatta cgtaagtgta actaatgaaa aaaattgtac
     3301 tttttctgta tataaagaaa attcttctaa taatataaaa aatggaaagc tagttgtaag
     3361 tgctaagaaa aaaatagata agaaagatag tgatttgcaa gatatgaatg gcgacaatat
     3421 gataaatgca aataaagata ggatggtaaa tgtgataaaa attggagttc ccgataaaat
     3481 gtattctaat tttaaagtag atgttgaaag tggttcagta gaactttata atattaaagg
     3541 aggagctact gttgatgtca atgacggaat tgctaacatt gttgataaca atgtaacaaa
     3601 tcctttcaat ataaatacta atagtgggat ttcaggtgta acagctgaaa ctatatcatc
     3661 tgaaataaaa tttagagcaa acaatggaat tgtaaatgta acagctacaa atataaatgg
     3721 caatataagt ttatttggaa gcgataataa agatactttt gatggaatat ttaaattaaa
     3781 tctgaaaaaa gaaccatcaa atttacactt gaagttaatt ggaaatggga taaataaatt
     3841 gccagaaggt tggtctaaag attatatttt aggaaatgga caaccaatta ttgaagttaa
     3901 aaataatggc ataaataata tatcaattgg agaataaaaa catatgaaaa taaagtttat
     3961 atagttttaa aaattataga attttaaaat atttatatat attctttaaa taaaaaatta
     4021 ttttt
//
LOCUS       DAMJWU010000192         4020 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMJWU010000192 DAMJWU010000000
VERSION     DAMJWU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.197"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            166..1671
                     /locus_tag="PC460_003632"
     CDS             166..1671
                     /locus_tag="PC460_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2602476.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1785..2483
                     /locus_tag="PC460_003633"
     CDS             1785..2483
                     /locus_tag="PC460_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2602477.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2768..3856
                     /locus_tag="PC460_003634"
     CDS             2768..3856
                     /locus_tag="PC460_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602478.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         1598 a          395 c          641 g         1386 t
ORIGIN      
        1 gatacaataa taatagaagt aataaaaaaa aataattaat ataaaaatgt gtacaaacta
       61 atgaatattg atatagtaag ctatcatatg aaagagtatg tacacatttt ttttactaat
      121 ttttcacaat atgaacataa ataatttaca ggaaaggaat cttttatgaa aattcaaaat
      181 actgagtggg gatatattga atggaaacat acttatgatg aaaataatcc aaaacaagct
      241 atgaatatat acattgcagt aactatgcct ggtaagaaac attttaatca tgttcattat
      301 ggtcaagagc agatgatata tattttagag ggagagggtt tgtatattat aaatggagtt
      361 tggaagccat tttatcaagg tatgattttt tatatagaat ctggtagcac acatgaaact
      421 ataaatacag gcgatagaga aataaaagaa cttattgttt caaataatgt agatgatgta
      481 ggagagagtg aagttataga tattaatcct aataattatt taaagaagac cttgataaat
      541 tatagtgaaa gtacattaaa tttgtatgca gcagtagaat caattcgtgg tcaatttata
      601 gacccgttta aaattccatt aattatatat gatgattcat ggaatatagt tttaaaaaat
      661 ccttattttc cacttttttg ttttgaaaaa tgtaatccta tgaaatttcc tcaaaattgt
      721 gattgtatga atcaaaaaag tagcaatcaa tttgtatgtg aatatgggat aactatatat
      781 aatataccaa tactgtacaa aagcaatagc ataggtgtta taagaggagg gtatgtacta
      841 ttatctgatt tgaatttaga tacagagcat aataatttat atgatattcc agagggagct
      901 gctagaagta taaaacgcct cttgaaacaa atatctaaaa atataataaa tttttgctct
      961 tttaatgata taagaaagga tttacaagaa aaagagaaga ctatagcaag aacatatcat
     1021 tatggagagc aacttgaaat gaatttaaaa gtagctcaag atatggttac taatttaaga
     1081 ataaatcatc actttttatt taatacatta aattcaatgg caagcatagc actagatgat
     1141 ggtagttatg atttgtatag tgcaataata gatttatcaa gaatgtttag atatacaatg
     1201 aggtctgatt taagatttgt agaattagaa tctgaaatat tatacattaa aaattatttg
     1261 aatttacaga aattacgtta tggagatgca cttaaagtaa aatatcttat tccagagaag
     1321 ttatataacc tttctgtgcc ttttaacttt attcaaccta ttgtagagaa tgcatttaca
     1381 catggattta gagatattga cacagaaaag cgaattgaaa taattgcaag attagattct
     1441 cagtatgcca ttatagagat tcataataat ggaactatct tagatggaaa taatatagac
     1501 aaaataaagg caggtattcg tagtaataat ggtcatggtt tgtctttaat atatacaaaa
     1561 ttcacatctg cttatggaaa taattttgat atggatataa agtcgagtga taatgaagga
     1621 acttacataa tgattaaaat tcctattgaa aattataagg agtatgtata gaacttgtga
     1681 aaaattaata agttttataa atgttaagaa atatattaga tgtttttatg ttgttttata
     1741 tatcttactt ttttatatta ttaagctaaa agggaggtat tctcatgaga agagcactaa
     1801 taatagatga tgaagaagct gttctaaaaa taataacaca ttttatagaa atgaaagata
     1861 tgccaataaa aattgttggt aaagcaacat ctggtgatga agcagtagat aaaatcattg
     1921 gtttagagcc agacatagtt tttatagata ttcaaatgcc aatatacaac gggttagaag
     1981 taatagaaaa aacatctcat ttatctaaaa aaataaactt tgtagtaata actgcattta
     2041 actattttga atatgctcaa aaagctttga gattaaatgt aaaagatata ctccttaaac
     2101 cattggatat gaaagaattt actaagtctg tagaaaaaat tattggatat caatatacca
     2161 ataatgattt attaaatgag atactggaat atataaatct aaactatagc aatgacttaa
     2221 agttaaatga ttgtgctaga ttatttttaa ccaatccatc taatatatct aggattttta
     2281 aaagtaattt aaatacaact tttatatcat atctaaatca tataagaata gaaaaatcta
     2341 ttgagttact tgaaaatact acaaaaagta taaatgagat tgcagaaata gttggatata
     2401 atagcctaaa taacttttat aaaaacttta agatagaaaa aggaatgact ccaaaagttt
     2461 ataaactgaa cactaaaaat tgaaattgat aaataaaaag tcaatataga taattgtttg
     2521 agtgtgtctg aataagtata ctttaattat gataaaaatt aaagcgcttt aaacatattt
     2581 aataatatta aaccttataa aagctgtacc tatttatata aaaaaatata tataaataaa
     2641 aagaaaaaga tatacaaaat aaaaaaagtg aattaaatac tgtttcaaaa agacagaatt
     2701 ttcatttaat ttactgagag tttatatttc ttaaaggaag gtaactttat aatggaggta
     2761 atttaatatg tcagacaaag tgaatatgaa aaatattatc tgtattgcag gcgcttatct
     2821 agcaacagca attggttctg ggtttgctac tggtcaagaa attctacaat tttttgcctc
     2881 acaagggtat atgggtttaa ttggagcatt aatatcaatg gtattgtttt catttcttgg
     2941 tgctgaagtt ataactagag gtagagaatt aaaacttaaa gaaccaacaa gaatttacac
     3001 tttttattgt gggaagattt taggaaaatt ttacgaatgg tttagtccaa tatttttatt
     3061 tgggattctt atagtaatga tttcaggtgc aggctcaact ttaactgaat actatggtat
     3121 aaatccttac ttaggaagag tattgatggc tgttgctact ttaattacag tgtctatggg
     3181 tttagaaaaa ttagtaagca tacttggacg tattggacca gtgatagcag tatttacaat
     3241 tgctgttgga ttaataagct tgtttaagca ttttgacggt ttatctatgg ttggagaagc
     3301 tatgaaaaat attgaagtta caaaagcatc agaaagtcca atcgtttctg gtgtaatata
     3361 taatacatta aatattattg taatgattgt ttttttaaca ggaatgggag cttctattga
     3421 aagtaaaaag gatgcttttt ggggaggttt attaggtgga atagtgttta tgactgctgg
     3481 tatagttatg tatttggcta tgatttcaga tataggaaat ttgtatatga aagaaatacc
     3541 atcactttat ttagcagata atatatctcc gattgtaggt gtatgttttt cagttgcatt
     3601 aatattaggt atctatacaa cagctgttcc acttttatgg tctgtaacca atagaattgt
     3661 tgaagatgaa catcctaaat ttaaattggt aacaacagta atagctattt tggcttgtat
     3721 aggaggtttt ttaccgtttg gtaaattagt aaacatttta tatccatata caggttatat
     3781 ggggctttta attttagtct gtatggtgta taaagcaatt acatgtagaa aaaaatcaac
     3841 acaaagtcag gaatagagcc acttattaat agtttttatt taaaaaaatt ttttatataa
     3901 tccgaattta aaaagtcaaa atagttaaat aaattgcaaa acatgataat atatttaatt
     3961 aaaataatat agtaaaatat aaatataaac taaaattaaa tttataaaaa atatctttat
//
LOCUS       DAMJWU010000193         3826 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMJWU010000193 DAMJWU010000000
VERSION     DAMJWU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.198"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            27..695
                     /locus_tag="PC460_003635"
     CDS             27..695
                     /locus_tag="PC460_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602479.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSGKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            854..1360
                     /locus_tag="PC460_003636"
     CDS             854..1360
                     /locus_tag="PC460_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2602480.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1488..2504
                     /locus_tag="PC460_003637"
     CDS             1488..2504
                     /locus_tag="PC460_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2602481.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2858..3562
                     /locus_tag="PC460_003638"
     CDS             2858..3562
                     /locus_tag="PC460_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF2602482.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1642 a          326 c          658 g         1200 t
ORIGIN      
        1 ataaattttt taaagggtga ttttttatgg aaaatataac tagttatttt acaacaatac
       61 tatgtgtatt tatatgctta tcatctgtat ttatatttac tcaactagct agagtattta
      121 ttaataagaa gaagattaat caaaaaatta aatcaagaaa cggatttagg tatgatagag
      181 attttattga agccagaaga gaagaaatac atataaagga taacaataat aataaaaata
      241 agagtaacaa taagaaatta aaggaagaaa aagtatttaa atacgataat ggtgatttat
      301 ataaaggtga gtttgttgat ggaaagaaaa atggatttgg aatatatatt ttttcaggta
      361 aggaaaagta tgaaggatta tggaaagatg acaagatgca tggaatagga aaatatactt
      421 atagagatgg ttcaatatat acaggtgaat ttaaatacgg attaaaaaat ggtttgggaa
      481 aattaacata tcccaataat gacatttata agggatattt tttagataat aagaaaaatg
      541 gtaagggtgt attatataaa aatgatggaa ataaacaagc aggaatatgg gaagatgatg
      601 aacaatgtaa aagtttagat tttaaagatt taaataataa taaaataaat aatgtttatc
      661 aaaatagatt aaataacaat aaattaaaag tttaattatt ctaataaaaa aatctttatt
      721 tatgatggaa tataggatat atctatgttg taatttgtaa aatcttccta tatcatataa
      781 aattaagtat tgattagtct attacataaa tatagaatat aaatatatcc atttaataat
      841 aaggagaaga gatatgaaag agtatgtagt tgtggattta gaaactacag gattagatcc
      901 atataaaggt tgtgaaataa tagaaatagg aataacagaa ataaaaaatg aacaaattgt
      961 aaaaaactat tcaaggttga taaaacctaa ggggataatt tcttcattta taacagaatt
     1021 gacacatata agtaatgaaa tggtagaaaa tgaagaacca ctagaattag ttttacctag
     1081 atttagaaaa tacataggtg atagaactat aattgcacac aatgcgaaat ttgatttaaa
     1141 atttttaaat tactatctta gaatgttaga tttagaacca ataaacaatt atatatgtac
     1201 agtagagtta ttaaaaaaat gtaaaagtta taaaggaaaa aacaagaaac ttgaaacagc
     1261 ttgtgcttat tacaacatag agaatataaa tgctcataga gctgacagtg atacattagc
     1321 aactgcaaaa ctatttttga aaataaaaga tgaatattaa ttataaatat agagaaaatt
     1381 agtgaaaata tcctttataa aattgaccct taagcttaca attttgttct ttgatttatt
     1441 ttgtagaaca atgtataatg taccttatat gaagaaggga gactaaaatg ggttattaca
     1501 atgataatga taataataaa ataagacgta aaaaaattag taacaacaaa gatagtaata
     1561 aaaaatctaa acattcttat acttcggcaa ataaaaaaaa taaaagtcct aatacaagtt
     1621 ccagtaaaag aatgagtgaa aaagtagatt cacagaatct tataaaaaga gatagagata
     1681 gttttacaag agataatgta agacctttaa atacagatta caatcaaaga gaaagaagaa
     1741 gagtcgagta taaacaaaga gagaaaaaat taaaaagtaa aaaaagaaaa aaacaaaaaa
     1801 ggattagaat agttagggca ttattatcta ttatgttagt tgcactaatt atattttcag
     1861 tggcaggtgc aatatttaca gtttcagcta taaaagggtc accagaagtt actaaaaagt
     1921 taattaggtc taaatatata agtagtgagg ttgtaagtat aaaacaaatg ccaaatgatt
     1981 taaaacatgc aatagtttcc atagaagatg agagatttta taagcataaa ggagtagatg
     2041 ttatttctct agttaggtca gtattaaaca atgtgtttac agatacaaca caaggtgcaa
     2101 gtactataga tatgcaagtt tcaaaaaacc tgcttactag taatgataaa actatgaagc
     2161 ggaaaataag ggatatgtat aatgctaagc agatgaataa gattatgact aaggatgaaa
     2221 ttcttgaagc atatttaaat aatatgtatc taggtaaaaa tgcatatgga gctgcaaagg
     2281 gagctgaagt ctactttggt aaaaaagtta gtgaattaaa tttagctgag tgtgctatgt
     2341 tagctggtat gacaaataat cctgcaaggt atattgacca tggagaggct aagaagagac
     2401 aagtgattgt actgtataag atgcatcagc ttggttatat aactgataag gagtatagaa
     2461 tagcaaaagc tgatgacact ccatttaaat cagaaattga ttaaaattgt aagaatatag
     2521 tattttcaat agtttaaaca aacaaaaatg taataaaaaa ggtgatttgc cacccatttg
     2581 ccaccataat tataatctaa tggcaaattg ttttaatgta caaactagat aatagaatat
     2641 tgtcttaaat gctatattat gctaaaattt aattgtattt tgtaaattaa aaaacttata
     2701 atttagtttt ctagggttcc gcattttata atggtcaggt ccaagagaaa acgcgcaatt
     2761 gttgtgtaca cggaaggata aaagcctggg agatatttaa gaatatttct ataggctttt
     2821 ttatttttac atattttaaa tttgaggagg ttttctaatg aaaaaatttg ttattgagga
     2881 tgcattttgg aatctatttc ctagtgcaaa aattggagtt gttatttgta atgatataga
     2941 taactctata aaagatgaag actataagaa aataattttg aagggagaag aagaagcatt
     3001 aaaatacttg gaggactcag aatttagcaa caaccaagtg attaaagtat ggagagaagc
     3061 atttaaaaaa tttaaaacga aaaaaggagc aaggtcttct attgaagcat tattaaaaag
     3121 agttcataat ggaaataata taggaactat taacccttta gtggatatct acaattttat
     3181 ctcattaaaa tatgcattac cttgtggagg ggaggatata gataaattta taggagatat
     3241 taggctaact agagcaattg gtaatgagga gtttatccca ctagggacag atgaaaattc
     3301 atttccatat gaaggagaaa taatttataa agatgatggt ggagcaattt gtagatgttg
     3361 gaattggcgt gaagcagtga gaacaatgct tactgaagat actaacaatg cctttttatg
     3421 cattgaatta gtagatgaaa gtagatttaa agaatttgaa aatgctttaa atgaattagc
     3481 taaaatagtt caagaaaaat tgggtgggaa ttgtaaaatt tcaattttag atattgataa
     3541 taaagaggta tcagtggatt aatatggagg tgctgttgta atggactatt ctagttataa
     3601 agatacttat atttagggta agaaggtcta ttttagatat aacgattatc aaaggatata
     3661 atatagaaaa taaaaatgta gaatataaaa agcctttaaa ataagaaaaa aatgtatttt
     3721 aaaggctttt attatactct tttttatttg cataattaac aataaaaata tattgttaat
     3781 tataatattt ttatttaaaa acgatataat ataatgtatt atacta
//
LOCUS       DAMJWU010000194         3801 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMJWU010000194 DAMJWU010000000
VERSION     DAMJWU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.199"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            78..1130
                     /gene="ytvI"
                     /locus_tag="PC460_003639"
     CDS             78..1130
                     /gene="ytvI"
                     /locus_tag="PC460_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2602483.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1220..2446
                     /gene="pepT"
                     /locus_tag="PC460_003640"
     CDS             1220..2446
                     /gene="pepT"
                     /locus_tag="PC460_003640"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF2602484.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPKIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2653..3705
                     /locus_tag="PC460_003641"
     CDS             2653..3705
                     /locus_tag="PC460_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF2602485.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADNISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         1477 a          393 c          700 g         1231 t
ORIGIN      
        1 tttattttgt attcaaataa aactagtatg aaatggacaa aataaaaata taaattagaa
       61 gaaaggagat ttttaagatg agcatactag aaaataattt tttatacaaa ttaaaaaaca
      121 acactatctt tttttgtata tatacattgt tatttatttt tatttacaaa gcgtttccat
      181 acatagctcc attttttcta ggtgctataa tagcacttat gattaatcct atatcacaaa
      241 agttagaaaa taagtttcat ataaataaag gtatctctac tcttgttcta agttttcttg
      301 cagtggctat agtcagtaca gttactacaa taatagtaat aaattcaatg aaagaattaa
      361 tgggattttt aaataatata tctgctaatc ctgaggatat aagtaatact attatgtatt
      421 ttttaaataa aataaatgac tttatgaaat catttcaaga aatagccaat tttgatgtag
      481 aacaattagt aaacaaattc agtggtgaag taatgcaaat tacaaaaaat cttcttacta
      541 gtatacttgg attggctact tctattccat atattataat atttataatt accttgttta
      601 ttgctacgta ttttatagca aaagaccttg ataagattga aaatagtttt tataatatgt
      661 ttactgttga tgttagaaag aaggtaaaaa acgtaaagaa agaggcaggg ctttcacttg
      721 ttggatatat aagggcctat acaatactta tggctataac attttttgca atctggggaa
      781 gttttgcttt atttggattg aaatacggtc taatagtagg gtttgtagga gctttaatgg
      841 atttaattcc attcctaggt ataataacaa tatatcttcc agtaattgtt tattattttc
      901 ttataaaaaa ttattttata gctataagta tgactgtgat attttttgtt ttgtctttga
      961 ttagagaaat acttgaacct aaactagtat cagtaaatgt tggactaaat ccattagcta
     1021 ccctagcagc gatttttatt ggaatccaag taaaaggaat aattggagta attttttgtt
     1081 tgggtttggt atgtatgcat gatatactta agaaggtgga tattttttaa ttataataat
     1141 tgatagattt tttaaaaata aaattttatt cacaggtaaa taaaaaaata ttaatcttat
     1201 taagaaagag ggtatatacg tgaaacaaaa agtagtggaa agatttttaa aatatgtatc
     1261 atttgataca acatctaatt ctcagtgtga gaactgtcca tcttcagaag gtcaaagagt
     1321 tttagctaag tatatagttg aagagctaaa aacaatgggt gttgatgatg tgagtttaga
     1381 tgaaaatagt tatataatgg ctactttaaa aggaaatact gatggtgtgg atactatagg
     1441 attcatatca catttagata caattgaaga tgttagtgga aaagatatta aaccaaaaat
     1501 aatagaaaat tatgacggaa aagacatagt tttaaatgaa gcgttaaatg ttattacgta
     1561 tgtaaaggat tctccagagt tggaagaatt taaaggtgac gatttaatag tcactgatgg
     1621 aacaactcta cttgggtctg atgataaagc tggtatagct gagatagtga ctgcaataga
     1681 gtatctaata aatcatccag aaattaaaca tggagatata aaaataggat ttacaccaga
     1741 tgaagaaatt ggtagaggtg ctgatttatt tgatgttgaa aaatttggag caaaatatgc
     1801 atatacttta gatggaggaa tagttggaga acttgaatgt gaaaatttta atgctgcaaa
     1861 tgcaactatt actatacatg gtagaaatgt tcatccaggt tcagctaaaa ataaaatggt
     1921 aaatgctata catatagctg ctgaaatatc agaaatgttc ccagcagatg aaagaccaga
     1981 aacaacagaa ggatatgaag gattttggca tttaaattct ataggtggaa atgtcgagaa
     2041 tgtaagtatg gcttatatta ttagagacca ttgtaaagaa aaatttgaaa atagaaaatc
     2101 tattatgata gagaatatag aaaaaataaa taagaaatac gataatagag tggaattgga
     2161 tttaaaagac tcttattaca atatgaaaga aaagattgag ccagttatgt ttatagttga
     2221 tatagctaaa gaagctatgg aggaattagg tataaaacca cgtttagttc ctgtaagagg
     2281 tggtactgat ggagcaagat tatcttttaa tggattacct tgtccaaata tatttactgg
     2341 tggattaaac ttccatggga aaaatgagtg tataccagtt agttctatgg agaaagctac
     2401 taaactaatt gtaagaatag ctgaaaaata tgctgaaaga gtttagttaa aaagtattta
     2461 aaaattaagc atttaaagtt ataatagcta tatcattctt tataagtcaa gctgattata
     2521 agcttgtgtt gtacttaaaa tggtatagct atttttgtaa ttatttttat aaaagataat
     2581 tatttttagt ttagggtatc aataaaaagt atgaatatca ataggtatta acatttaaaa
     2641 gggggaattg tattgaaaat taaatctaaa aaaataatag catttgcact ttcacttggg
     2701 attatgttgc caagttgtgt atatgcagac aatattagta aaaatcaaaa tgcacaggta
     2761 gatatgaaca attatcttca aggtagaaaa tctccaaagg aggaaaaact tattggtgaa
     2821 gatagatttg atacagcgat aaaaatatct caaagtggtt ggaataatgg ttcagaaagg
     2881 gtgtttctag ttaactcaaa ttcattgcca gatgcattgg cttcaactcc tttggcaagt
     2941 aaattggatg caccaatact gcttacaaat aaaaatagta taccatataa tgtaacagag
     3001 gaaataaaaa ggcttaatcc agcagaagtt attttaattg gaagtgaagg agctatttca
     3061 tcaagtgtaa aagaatctct tgaagatatg gaagtattag tcagtagaat cggtggagca
     3121 gatagagaag aaacatcttt acttcttact agacagctag atgatactgg tgatttaggt
     3181 gtctcaaaag tggcagtggt taatgggtat aatggactgg cagatgcaac ttcaatatca
     3241 agcccagctg caagtgataa tacagctata atatatacag gtaaggatag cataagaagt
     3301 gaggcaaaga attttattac acaaaactca actaagacat acatagtagg tggggagtac
     3361 agtataagta aaaaacttga aggacagtta gtaaactcag aaagactagc agggacagat
     3421 agaaaagata ctaatgcgaa agttttagaa aagttttatg gaaaatcaag taaggttaat
     3481 aatatgtatt ttgcaaaaga tggaagtggt agagaagctg atttagttga tgggcttgct
     3541 gctggagtat tagcatcaaa aacaaagtca cctgtagtac ttgcaagtgg aagtttaagt
     3601 agtgcacaga aaagttttat aaaaaaagta aaggctgata aatttgtaca agttggtgga
     3661 ggtaaaaata gtaaaccata tgcagaagca ttagccttac agtaatattg tgtaaatcaa
     3721 aattaatata taataatagc aagtaaacca agttgcactt taatatatat aataaaataa
     3781 gtaaactaag ttatatttca g
//
LOCUS       DAMJWU010000195         3698 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMJWU010000195 DAMJWU010000000
VERSION     DAMJWU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.200"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            194..1774
                     /locus_tag="PC460_003642"
     CDS             194..1774
                     /locus_tag="PC460_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF2602486.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1952..2398
                     /locus_tag="PC460_003643"
     CDS             1952..2398
                     /locus_tag="PC460_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF2602487.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2419..3462
                     /locus_tag="PC460_003644"
     CDS             2419..3462
                     /locus_tag="PC460_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF2602488.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1496 a          342 c          696 g         1164 t
ORIGIN      
        1 acataataac ttaaattata tggtataata aattaaataa ttgttttaat tcaaaatttt
       61 ttgttatttt aaattatttc aaaactctta gttttagtga ttttatttat gatatttatg
      121 ggattaaaaa gatttcaata gatatttttg gtaagtattt aagaagtctt taactttata
      181 ggagtgataa tttatgctaa taggtacatc taaattaagc agaaatgttg atactgcatg
      241 tgtagaagaa gtagggattc caatgattgt attgatggaa aatgcggtta tatctgcaat
      301 gaaaaatatg gatatagaca tgtataatag ttatacagtt gtatgtggtg ttggaaataa
      361 tggaggagat ggtcttggaa tagcaagaca tctaaataca tatggaaaag aagtaaacgt
      421 atttttagta ggtaatcttg aaaaactaag tgaatgctct aaaataaatt acaatgtact
      481 tttaaatatg ggaattaata ttataaatat taataaaaat gatttaagtg aaaaaaatta
      541 tggagaggaa aatgaagtag ataaaattat taaagttgag aaacaaaata ttataaaaaa
      601 taaaaattta acaattttta aagatagagt atctaaaagt gatgttgtag tagatgctat
      661 atttgggact ggattaaaac gtgatataac tggtattttt aaagaagtta ttgacatagt
      721 aaacgataat agcaaaaata cgtattctat agatattcct tcaggtatag atagcgataa
      781 tggaaatgta ttaggaactt gtattagagc aaacaaaacg attagctttg agttttataa
      841 aagaggtttt ctaaactatg acactagcgc acttataggt gatgtagttg tagaaaagat
      901 aggtgttcca gattttatta caagaaaata ccatgatgaa gagttcataa ctgataagac
      961 ttttgtaaaa aataatatta gaagaagaga taagcatggt tttaaaagtg attttggaaa
     1021 agtatctata gttgcaggtt caaaaggatt ttatggtgca agtttcatag caactgaatc
     1081 tgctgttaag agtgggagtg gacttgtaac attaattagt agtgaagatg tgcaagaaaa
     1141 ggtatctatt agacttacag aagcaatgac tttaaatttt gaagaagaaa gattaaataa
     1201 attacttaat tctagtgatg caattggttt tggtccaggt atgggagata atagtcaaac
     1261 atttgataaa ttacttaaga tagtagaaaa ttctaattgc ccaatagtat tagatgcaga
     1321 tggattgaac gttatgaagg acagatgtta taagttttta gaatggaaaa atagatttgt
     1381 aattacacct catttgggag aaatggccag gttgacagga gattcaattg ggtatataag
     1441 agaacataga gtagatgttg caaaagaatt tgctcaaaaa tataatctgg tggtgctttt
     1501 aaaaggttat caaactgtca taacagatgg taaaaaaaca tatataaatc ctacaggaaa
     1561 tagctgtatg gcaactggtg gaatgggtga ttgtttgctt ggaatgatta cttcatttat
     1621 aggtcaaggt atggatatat tggaagctac agttagtgga gcatatatac atggctatat
     1681 aggagataaa ctgagcaaag caatgtatac ggtaaatgct acggatttaa taagtaacat
     1741 ttcattaaca atgaaagagt tgttggatat ataattggtg atatttgata ctactttgta
     1801 acaaatttaa caaagtatgt tgatataata ctattaatta attaattaat ttattttatt
     1861 gttttgtaat ttaaatttat attcaaaaac attgtataaa catataattt atatagggtt
     1921 ttataattca tatgaaaaga gggtagatag tatgaagtta atatggttga tagtagcagt
     1981 cttatttgga atagcagaaa tgttaacacc aagcttaaca ttgatatggt ttagtgtagg
     2041 agctgtaata cttatatttt taagtagctt tatagagagt attttcttac aaattttaat
     2101 atttgcagta atttctatag caatgttagt cgtagctact aaaaaaatag taaaaaaaga
     2161 taaaggctat aaatctaaca ctaatttaca agcaatgatg tctaaaaaag gtattgttac
     2221 agaagagatt tctccaaata acacaggtct agttgtagtt gaacatgaaa catggacggc
     2281 tatttctata gatggtgaaa agatagaaaa aggttctaca gtagaagtat taaaaattga
     2341 aggggtaaaa ttagttgtta aaaaagtaga cgccacagtt aatgtgacaa accaatagaa
     2401 taaaggagga tttatagtat gggtactaaa attgttttaa gtattgtatt aatagtagta
     2461 gtcgtagcaa taagtttaac ttgtataaga gttataaaac aatctaaagt aggtataata
     2521 atgagacttg gtaagtttca aaaagtagct gaaacaggtg tacatttctt aataccattt
     2581 ttagataaaa tggcatatgt aattgacctt agagaaattg taatagattt tccacctcaa
     2641 ccagttataa ctaaagataa tgtaactatg cagattgata cagttgtata ttataaagtt
     2701 acagacccag ttagatatgt gtttgagata gctaatccaa tagctgctat tgaaaattta
     2761 acagctacaa cactaagaaa tataattggt gaacttgatt tagatgaaac attaacatca
     2821 agagatataa taaatgtaaa aatgagaact atccttgatg aagcaacaga taaatggggt
     2881 ataaaagtaa atagagtaga gttaaaaaat ataatgcctc ctcaggatat tcaagttgca
     2941 atggaaaaac aaatgagagc agaaagagaa agaagagagg caatacttca agcagaaggt
     3001 aataagtcag ctgcaatact tcaagcagaa ggtgaaaaac aatctgcaat attaacagca
     3061 gaagccaaaa aagaagctat ggtacgtgta gcagaaggtg aaaaagaatc tgctatatta
     3121 gtggcagaag gtgaagctga agctataaga cagacagcta ttgctaaggc acaaggtgaa
     3181 gctgaaatga taaaaagaac tcaaatggca acagcagaag gtttaaaatt agttttttca
     3241 gcaatgaaag aggctgatat agacaataat attctagcat taaaatctat ggaagctctt
     3301 gaaaaaatgg ctgaaggtaa gtcaacaaaa ctggttttac cttcagaagc agttaatttc
     3361 ctaggaacat tcaaaggaat aaaggaagtt atgagtgatg ataacaaaga agtacttgat
     3421 ataaaagaag ttttaaatga taatgaatca ttaaaaaaat aaaagagttt tgttatcata
     3481 ggaaggatat ggaaattaaa ggtcgaatta attaaatata gcacaaaaat gcacgttgaa
     3541 aagaagagga cttataaatt tttaagtctt cttctttgtt tttattagaa agatgaaatt
     3601 ataaaaataa attaacagaa tctttataca atttatcctt tactaatgta aaaagtgtta
     3661 tataatataa tataaaacat atagcaaata agcatggt
//
LOCUS       DAMJWU010000196         3626 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMJWU010000196 DAMJWU010000000
VERSION     DAMJWU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.201"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(233..1249)
                     /locus_tag="PC460_003645"
     CDS             complement(233..1249)
                     /locus_tag="PC460_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2602489.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            complement(1432..1794)
                     /gene="spoVAE"
                     /locus_tag="PC460_003646"
     CDS             complement(1432..1794)
                     /gene="spoVAE"
                     /locus_tag="PC460_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF2602490.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            complement(1808..2821)
                     /gene="spoVAD"
                     /locus_tag="PC460_003647"
     CDS             complement(1808..2821)
                     /gene="spoVAD"
                     /locus_tag="PC460_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF2602491.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            complement(2988..3425)
                     /gene="spoVAC"
                     /locus_tag="PC460_003648"
     CDS             complement(2988..3425)
                     /gene="spoVAC"
                     /locus_tag="PC460_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF2602492.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
BASE COUNT         1179 a          643 c          386 g         1418 t
ORIGIN      
        1 tagattcatg atataaaata caaataattt attaatttat attgttaaaa taacggattt
       61 tcaaataatt gaataaaatt attcattcat attataaaaa ctatctcaat aaataaagga
      121 gtacaatgta cttatataca cctactcctt ttaattttta tttatattat ttgttaaaaa
      181 acttcacata atctataata tttttaataa acgtttgata cttaaaagct tactacttta
      241 ttcttttaac gctatttcgt attattaacc ttggatataa ttttctcgga atgtgttctg
      301 tactcttttt atttattata tttactaaac tatctactcc attttttata acttgctctc
      361 ttgggtcatc tattgttgat aaatgcgacc taaaatatga tattaattca atattattat
      421 accctattat ggaaatatct tctggtacat ttataccata agtagttttt aactcttgta
      481 ttatagaaat cgctggtaca tcttgttgta caaatatagc tgaagctttt tttattttat
      541 caatgtattt ttctaaaaat tcaacttgag attcaaaatc cattttagat ttaatgactt
      601 ctgtctttaa atttgcttct tccatagcat tcaaatatcc cttggttcta tcttcatgca
      661 tttttgaact cttatcatct gaagttactg ttattatatc tttatgacca tggtcaatta
      721 aatgcttggt agctatataa cctccataca cgttatcgtc ccaaaagaaa ttatctactt
      781 ctcctataaa agatggttta aaatatataa atacatgtgg aaaattattg tcttttaaaa
      841 aatcatactc ttcttttttt attgattgag acacaattac taatccgtca accatttgtc
      901 catttaccat tttatataca ttactatcac cactaatgtt attattaggc tgtattataa
      961 aatcgtattt attagtctca atagaattta tagaactatc cattatatca ctaaaaaact
     1021 ttctagtatc ttgatgatta aaattatttg aaaagattac ccctatcctg ttagtttctt
     1081 tttttgctaa atttcttgca tttacattaa aatggaaccc tagtctattt gcctcttcta
     1141 ccactttctt ttttgttttt tcagatacat taggattatt gtttaagcac ctagaaacag
     1201 ttgaataact gatacctaga ttccttgcaa tatctttaat agttaccatc tttatcccct
     1261 acttcacttc attttatttt gataatataa tactacacta tgttgtaaat aattgtcaac
     1321 ttcaattatg taaacgcttc tttatataaa aaacaggtgg aatctaaaag atatccacct
     1381 gctttataaa aatttattat ttcaaaaact tattattctt ttaataaaga tttatggttt
     1441 tgcttttgga gtaaatacta ctgacataat atatccaaag aatatagctg ctgctattcc
     1501 accagctgta gcagttgtac ctcctaaaaa aatacctaaa aaaccatcac tttgaattga
     1561 ttttattact cctgtagcaa gtgaatatcc aaatcctatt ataggaactg ttgcacctgc
     1621 tgctgcaaac ttaactaatg gctcataaag tcctagaaaa gttaaaacta caccagttgt
     1681 tacatacgtt accataacat atggagtttg cattttgaaa taatccatca ttacttgggc
     1741 tattaaacat ataattccac ctacaataaa tactcttaca tagtccatta acatattata
     1801 tcacttccta ctcatttact attactactg catgtgcaac acatggtatt gtctgctttt
     1861 gtagtgtact tgtcggtgaa agtagtgctc ctgttgatac aagcattact ttgttaaatt
     1921 ctttttttct caacttatca tatatatatc ctgcaaacac tgtggctgaa cacccacatc
     1981 cacttccacc acagtggaca tcttgttctt taaggttaaa catctctata ccacagtcag
     2041 tataaacttt tgatatatct acaccttttt cttttagaaa gtcttctgct atctgttttc
     2101 caagagtacc taaatctcca gtagcaatta tatcaaagct atttgggtca tcattagtat
     2161 cttcaaagta agagtagata gtatctattg ctgctggagc catagctggt cccatattat
     2221 taccatcatc aataccctca tctattactt taccaacagt aacatacttt acttttggac
     2281 cgtctccatt aggagctaat aatacacttc ctgctcctgt aactgtccac tgggctgtca
     2341 ttggtttttg atttccaagt tcaagtggga atctaaattg tctttctgct gtacaatagt
     2401 gacttgatgt acctgacagt actaaatcag caaaaccacc atctataagc atagaaccta
     2461 tacaaagacc ttctgccatt gtagaacaag ctccatatat acctaaaaat ggaatagcca
     2521 gttctcttgc tgcaaatgat gctggaacta tttgatttat taaatcgcca cctagcatat
     2581 aatttacatc tgacaatttt ttacctgcct tttggatagc ttgttgagat gcagtctcta
     2641 ccattttact ttcagctaat tcccaagtct tttcaccata taaatcgtca gtcataatca
     2701 tatcaaaata gtcctttagt ggtccttcac cttccttagg acctactata gttccagttg
     2761 atattatggt tggtttattt tctaacttga ctgttctttt tccaattctt ttatttttca
     2821 tattttcaca cctttttaat tttgcatttt actattcaaa tttttaatat taattaaaaa
     2881 ttttaattaa atctgcacaa ttatttttca attagttact ttagtagcta ttttcagatt
     2941 ttataccttc ttcataatta ataaaattta aattaaataa aaatcatcta aaacatctta
     3001 aaaatataat aaataattcc acataaaata gaagaaccta taccataaac taaaactggt
     3061 cctgcaattt taaataagtt tgcacctaca cctaatacat agccttctct tttatactcc
     3121 attgctggag atactattga gttagcaaaa cctgttattg gaattataga accagctcca
     3181 gctctttttc ctattaggtc gtaaactcct aatccagtta aaaatgcccc tataaatatt
     3241 aatgttattg atgttgcaga actagcacct agtttatcaa gacctccaac tttcatatac
     3301 aaatcattta ttgcttgccc tatcatacaa ataattccac ctacaataaa tgcaagtgta
     3361 tagtttttta aatatgtagg ttttgggctt atttggtcta catatttttt ataattttta
     3421 tccatactaa acacctctct aaagtattat ttgttaattt taaaaaaata aacctattat
     3481 ttattttgac tatctaaagt ttggtcaaaa aataaaaaag atgcctaata aagaaaaaat
     3541 ctatttttcc ataaagacac cttgttttta tatttttact tttttaatgc tttttattat
     3601 tttaatatct tttaataatt taaaaa
//
LOCUS       DAMJWU010000197         3543 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMJWU010000197 DAMJWU010000000
VERSION     DAMJWU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.202"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(36..1634)
                     /locus_tag="PC460_003649"
     CDS             complement(36..1634)
                     /locus_tag="PC460_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2602493.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTIILVNTDNSIVDGLSASGLSGVAKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKANIKGNVDINKAGTYVLNISVVDNLGQKSEISVNVKVVVNASTKDSNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKSLRELDSLAGMANAWSKYMEDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYTSKDAKEMAESIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            complement(1853..3454)
                     /locus_tag="PC460_003650"
     CDS             complement(1853..3454)
                     /locus_tag="PC460_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF2602494.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASNSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLLVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         1040 a          743 c          373 g         1387 t
ORIGIN      
        1 tactatatct tttccaatgt aaattaatat aaaatctaac tatttaaaaa ttccattgtc
       61 gcattcactt ctcctttact agaaacatgc ataccaaatc caaagctata aaattcttct
      121 tttaacatat tttcattata cttagaagac ttcttccata aatcaaaaat agactcagcc
      181 atttcttttg catctttaga agtataaaca gatttaacat tcattggtag atatgcaata
      241 ttttcaccac ttcttgctcc aaagccaaaa aatacttcag ctgcattttt accatcaatc
      301 tcatgggcaa ataccttctt atcttccata tatttagacc atgcatttgc cattccagct
      361 aaagagtcta actctcttaa actcttcttg cctttttctt ttctatatga attaactaaa
      421 tcatacatct cattagatac catagcctta aactcataac tattagagtc ttttgtactc
      481 gcatttacaa ctaccttaac atttacacta atctcactct tctgacctaa attatccact
      541 acacttatat ttaaaacata tgttccagcc ttgttaatat caacatttcc tttaatattt
      601 gcctttaaat catttccata ataatcagta gcaactatat tcaacatact attatcaaac
      661 ttatccccta cagaaatctc tacatcatta gcagtaattg taggcattgt atttttatca
      721 gatgcagaac taatacattg gtcaacaacc ttcttatcca ttccacctaa agaagtaact
      781 tcatcagcac ctctaagcac actcttatca cttccatcat taacaagtac tataggtttg
      841 tccttagcca acggagaacc agccacagca tcaactaact tatacccttg agaaacataa
      901 aacttctttg ttcctttata aaagcgttgt ataaccttct tattagtttc aaacctatcc
      961 ttaccagcaa tcctaactga attagtctta ctcacaagtt cattactcat tatctcctct
     1021 gacccaagag aatagcactg aacgccatca actttaaatg gtatactctt tccatcagta
     1081 agtatcacag gaactccatc tctagcagct acagaagata cactcattgc atcagcctca
     1141 cctgtgtatc catttacaag aaaaacctta tcaccatcat ttactggttt aatagctgaa
     1201 atctccttag ctataagata gctagtcttt attctatctt ccccaccaat tcttttaact
     1261 tcaatccctt tattcttaag ttgattttct acagatttac ctattgtatc ctctgtacct
     1321 ataacataag cattcttaac atcctttagt cttttttcaa catctgttgg tatcttatct
     1381 ctttgcacca acattatagg agcttttgca actccagata atccacttgc acttaatcca
     1441 tctactatag aattatcagt atttactaat atgattgtat catatatttg tttatctgct
     1501 atcaatgcag aagtctcata tctatctttc ccctgtatct tttctatgct cgaaagtgca
     1561 ttgatatttg gtgctcctgc cattattaat gatattgtca atcctattga taatactctc
     1621 ttatttacct tcatcaaact cctcctacta atttttttat ataattcagc taatcataaa
     1681 ttacatcctc agtagtgatt ccctcaaaat ctataaacta ctaaaacaaa aaacacctaa
     1741 aataccatag cctgtaaagc aataacaaaa cagattacaa aaagtacttt agatgtgatt
     1801 ttcattttat aatatcagta aattaacaaa aaaacaagat ttatggaatt ttttacaagc
     1861 taccttcatt tccatttaaa aactcctgag tagcatgcac ttgaccatca gataatatat
     1921 aaagcccaaa tccagtacta taaaactcat tactaagcat gtttgcattg tactctggag
     1981 acttcttcca cacctcaaat atagcctttg ccaagtcttt tgcatcttgt gtcgtttgaa
     2041 cgttcttaga atctatataa atatatgcta tattttcctc acttctcatt ccaaattcag
     2101 aaaatacttg tggagcattt tttccatcta tataatgtgc aaatactttt ttatccatca
     2161 tatacttaga ccatgcattt gccatacctt ctagcctact agacactagc aaaggctctt
     2221 tgcctttttc ttttctatag gaattgatta agttatacat ttcagtagat accatttttt
     2281 taaattctgg gctgttgtaa tcatgagatt tgtcatccaa cacttttatt tctactcttt
     2341 ttcctgtgct ttttccccat tcgtctgtag cttttaatgt caatatgtat gtacctactc
     2401 tatttgtatc tataaaccca tcaacttcta ttggtaatac cttccctgtg ttatctttag
     2461 ctactatatt taacttgcca gtgtcaaact tttcaccctt atatacctct gtacttccaa
     2521 ctgtaattgt tgggggttgc ccatttgaat ttgaagcatt tatacactgt tgtattacct
     2581 tctcatttat ttcacctaca gaggttatat tcttagctcc ttctaataca gttttatcgc
     2641 ttccatcatt tactagtacc attggagagt ttttagtaag tggtgcagct gctattgcat
     2701 ctactaattg gtatccatct gatacataga atccatctgc actattaaaa aagtagtcta
     2761 ttacgttttt gttagtttca aatcggtcag ttccttctat tcttactgag tttgtatttt
     2821 taactagagg attactcatt atctcctctg aacctataca atatgattga atatttttta
     2881 catcaaaagg cacactcttt ccatctgtaa gtatgattgg agctccatct ctagtagcta
     2941 ctgatgatac actcattgca tctgcttctc cactatatgc attagttaat agtactttat
     3001 ccactttttt tatagttgct atttctttag ctattaaata gcttgttttt agtctatctt
     3061 ctccaccaat tcttttgact tctatatttt tagaatctag ttctttttct actgatttac
     3121 ttatagtatc ttctgtacct attatatatg cttttttgat attttttaga catctatttg
     3181 tgtctgctgg tatcttattt tgttgtgtaa atagtattgg agcttttgta gctcccgata
     3241 atccacttgc acttaatcca tctgcaagtg atttttctgt atttactaaa attactgttt
     3301 cataagtctg tttatcagct atctttgctg ctgtttcata tctatcttta ccgtatattt
     3361 tttctactac tgatgatgca tttacacttt tgaagtttac taagattaat gatactgcta
     3421 gacctaatga taatattttt ttgttcattt tcatatcccc ctgatgattt ttatatttta
     3481 tatttatatt gtgattattt tccatttatt ttctctttat tttccatata ttatagagta
     3541 tat
//
LOCUS       DAMJWU010000198         3478 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMJWU010000198 DAMJWU010000000
VERSION     DAMJWU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.203"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            316..963
                     /locus_tag="PC460_003651"
     CDS             316..963
                     /locus_tag="PC460_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2602495.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(1130..1699)
                     /locus_tag="PC460_003652"
     CDS             complement(1130..1699)
                     /locus_tag="PC460_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2602496.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1704..2603)
                     /locus_tag="PC460_003653"
     CDS             complement(1704..2603)
                     /locus_tag="PC460_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2602497.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGILLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2614..2763)
                     /locus_tag="PC460_003654"
     CDS             complement(2614..2763)
                     /locus_tag="PC460_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602498.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2756..3340)
                     /locus_tag="PC460_003655"
     CDS             complement(2756..3340)
                     /locus_tag="PC460_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2602499.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         1235 a          581 c          380 g         1282 t
ORIGIN      
        1 tataaactta taaaatttaa acaagttatt cataaaataa aataataatc atctataacc
       61 cacaacttta ttttatatta aataaaaagt attagtgatt atgttttata ggtatttctt
      121 tattcagtta tttattaaaa actaatagta aatcttatac taatcgcata tttatgttat
      181 aactatattg taactatttt cgtaatctta ggaaaagatt acttataaaa ttaaccttat
      241 acaataatat atcgtataat cttaattagc ataatagtta caatattaaa atttaaatat
      301 gggagtgatt ttagtatgat aagacaaaca ccattagaga aacgatatga caagctaggt
      361 cctcatattg taacagcact caaaaaacgt tattttgatg cttattactg taaaactaga
      421 aatgaagctt tgcaacaaat tttagattta attcctcaaa atcatgtagt atcatgggga
      481 ggatctgaaa cacttaaaga aatgggcgta caaactgctg ttagagagaa aggatttaaa
      541 gttattgata gagatttagc aaaaactcca gaagaaagaa ctgaaattat gcgtcaagca
      601 cttctatgcg atactttttt gatgagtagc aatgcaatta gtgaagatgg acaactcttt
      661 aatattgatg gaaatggcaa tcgtgtagct gcaatgatat ttggtccaaa aaatgttata
      721 gttgtagctg gtatgaataa aattgtaaaa aacattgatg atgcaataca aagaactaga
      781 acaattgcat ctccagcagt tgttcagcgt tttcaagatg tcaatacacc atgctatatt
      841 aatggaagtt gtatagattg cacaagccct gaaagcattt gtgcatacat ggttactaca
      901 agaatcagtc gtccagcaaa aaaaataaag gtaatattgg taggagaaaa tcttggtcta
      961 taaaatatac atacatgtaa atagctctat aaataataaa atcatatgaa ataacttatt
     1021 tctaaaagtg gtagaataaa agcttctaca agactttcat tctaccactt ttatattctt
     1081 ttaatttgtg tttctatatt attaattgct ccgattttca tatatattac taactatttt
     1141 tattttcata atttttttgt tcttcatcaa gctgtttata ataatcttta ttatagtcag
     1201 ctagataatc tataccacct cctgtaatca catttaataa tttcattgca gattcttgat
     1261 taactttaca aacataatta tcaccagatt taacaagctc taatgtaata ttaaattctt
     1321 ttttctttgc agaatcaata tctttcatag ttggatttgg catcatttct cctagtttag
     1381 gaggattctt ttttaaaata gaaaccatat cagtattgtt aatttttaaa tctacagtca
     1441 ctttatcctt attattcttt atatctgaaa tttcccaagt catattttta gaatacattt
     1501 taaatccttc tttatcttcg tatataagat tacctaaccc tgattttggg aaatacttat
     1561 caacagtatc tgcatcttgt gtttttattg aattaaacat actatccact tcatttttta
     1621 tcttattatc attagtacaa ccagctaaca ccagtactga tattgcaaac attataatac
     1681 atataataaa tttcttcatt aaattatatc cccctatact tttttgcaca aatattattc
     1741 tcttttttat cttttgaaaa agttgtagta attgcatttt gtggacaaac ttttatacat
     1801 tcaccacacc taatacagtc tggactattt acatctttat atattggtat atcaagctta
     1861 cattttgttt gacatgctgt acaatttgta catttatctt tatcaatttt aaatctatat
     1921 attgaaatag gattaaaaaa accatagatt gctcctaaag ggcatatata cttgcaaaat
     1981 ggcctataag taatgattga catcaataat atcaatagca aaatacttaa cttccagcta
     2041 aataaaaatc ctattgattc tcttaaattc tcatttccta aaataagtag tattcctccc
     2101 aaaagtgttc ctgaagggca aatccattta caaaaccaag gatttccacc ccctgctaca
     2161 tttacaatag caactggcaa aataattaca aacattatta agactatata ctttagatat
     2221 tttagatatt tatcaccata aacttttttt atctttactg gaaagggtac tttatgtagt
     2281 aaatcttgta caagtccaaa gggacaaagc caaccacaaa caaatctgcc aaaaaaagca
     2341 ccaaatgcca ttaaaaaacc aacaacataa aatgagaatt tatactctct gcttgataaa
     2401 actgcttgta atgaacctat tggacatgct ccaagtgcac ctggacatga ataacaattc
     2461 aatccaggta cacataattt tttactttct cctccatata tagttccttc taaaaatcca
     2521 tttacatatc catttgttaa tgctgtaaat gcaacttgag taataagtct tatattatta
     2581 tttaccttag ataataactt catccctctt ctcctaacca ataccaatac actcaagaca
     2641 aatcataatt gctttagtta atactgtatt tgtttctcca cgatatgtac ctaatgcaat
     2701 aaatataata ctaattgcaa taatagaaat gcctttttta ttatctttaa taaagttact
     2761 cattggcttt taccattttt agtctttcat ctataatttt tttccaatct tccttactat
     2821 ttgcacctac aaccttctca cccacaaagt ttcctttctt gtctacaaaa taagttgttg
     2881 gaaactccat aactctttta aaatcagttt ttttaagttc ttcagataca gtcaattgtt
     2941 ggtaagttgc atctgacttt ttcataatgt ctttaactaa atttttctct tcatttgaaa
     3001 gaccagttct catatcagtt ttatcaactt ctacaactaa tccctttata gctacattgg
     3061 aattattact ctcatattct tttgataatg cttcaagctc tggcatttca cccacacatg
     3121 gtccacacca agtagtccag acattaataa gagtaagttc tttatttgaa aatatctctt
     3181 ttgttacctt atttccatca acatcctccg tatctaaatt actaaaccat tcactattat
     3241 tcttgttttt ttctactggc ttagattctt ttttgctaca tccagaaatc atacctaaaa
     3301 cacaaataca tcctaaaact actatgaaaa accttttcat atctcacact cctagaattt
     3361 atatagtata taaaatcgct gcttgaaata aaaataactt atcttctata ttaaagtaat
     3421 tatatttaat aaataagagc taatttaaaa aaagatatac agttaaaatc tatttctt
//
LOCUS       DAMJWU010000199         3330 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMJWU010000199 DAMJWU010000000
VERSION     DAMJWU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.205"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            94..849
                     /locus_tag="PC460_003656"
     CDS             94..849
                     /locus_tag="PC460_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2602500.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            946..1983
                     /locus_tag="PC460_003657"
     CDS             946..1983
                     /locus_tag="PC460_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2602501.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            2792..>3330
                     /locus_tag="PC460_003658"
     CDS             2792..>3330
                     /locus_tag="PC460_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602502.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMIS"
BASE COUNT         1261 a          435 c          502 g         1132 t
ORIGIN      
        1 ctttcctagc tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta
       61 ccaaaaataa atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag
      121 agtttcccta atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata
      181 gaagatggtg aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta
      241 aatataatat ctgggcttat aaaagaatct tctggtcaaa ttactttaga tagtactact
      301 ataacaaatt tagctgagta taaaagaact caaatagtaa gtagagtttt tcaagaccca
      361 agccttggaa cttgtccttc tatgacggtt agagaaaatt tatctctagc acttaataaa
      421 gggaaattat tgaacttaaa aaaatgtctt cgtcataaaa cgaatgactt ggaacactta
      481 cttgaaggaa tttctttgga tttaaaaaaa tatttagata ttaaagtaca atatctttca
      541 gggggtcaaa gacaatcact atcactaatt atgtctagct tagcaagtcc taaggtctta
      601 ttactagacg aacatacagc agcacttgac ccaaaaacat caaatgaagt aatagagtta
      661 acagataaaa tcgtaagaga gaaaaatata actacactta tggttactca caacttgaaa
      721 cacgctcttc aatatggaga taggttgata atgcttcata agggtgaagt tgtattagat
      781 gtaaaagata aagaaaaaaa agatttaaca gttgaagaaa tcttagaaaa atttgaatat
      841 gcagtataag aaattaaaat cttagtttta atcagaaata tttataatct atcatatgaa
      901 tatatgaaca aatataatta ttaaatttta agggaggaaa ataaaatgat aaaaagtaaa
      961 aaaatattaa gtttaattat agcaggagta cttggtgtat caatgcttac tggatgttct
     1021 caaaatgatg gctccaatgc ttcaaatgaa aataaagaaa cagatagcaa aaaacaaaaa
     1081 aatataggta ttactcaatt agtggaacat ccatctctgg ataaagcaaa gaaaggattc
     1141 atcaaagcac ttgaagataa aggctataaa gatggagata atataaaaat agatttccaa
     1201 aatgcacaaa atgatatgcc tactacacaa agtattgcta gtaagtttgt atccgacaaa
     1261 aaagatttga tatatgctat atctactcca tctgcacaag cagcttataa tgctactaaa
     1321 gatattccaa taataatgac tgctgttaca gaccctgtag aagctggact tgttaaatct
     1381 cttgaaaaac caggtggaaa tgtttctggt acatctgatt atctttcaat tgataaaaca
     1441 ttagaattgg ttaaaacatt gactccaaaa gcaaagaaaa taggggttat atacaatact
     1501 agtgaagtta attcaaaaat ccaagttgat tctctacatg attatgctaa gaaaaataat
     1561 tatgaagtag ttgaaaaagg aatcagctct tcaagtgaag ttaaccaagc tatttctagt
     1621 ttagttggta aaatagatgt tttatatgtc cctactgaca atttaatagt ttcttctatg
     1681 ccaatagttt ctaaagttgc taatgaaaac aagattccta taatagcctc tgaagaaggt
     1741 tctgtatctt ctggtgcttt agcttgttgt ggaatagatt atgaaaaact aggttataag
     1801 gctggagaac ttgctattga agtattagaa ggaaaatctg ttggcgatat accagttact
     1861 acattagatg aaactgaaat aataattaac gaagatacac taaaagcact tgacatgcaa
     1921 aagttatcag cagataatat aaagtatata aagtcagatg aaaatgcaaa atctacaaaa
     1981 taaaacttat taatttcaaa aaataggtaa tatgaagtag gtaatatgaa aactttatat
     2041 aataagtact cttatccacc ctaacccttt gaaaattgtg cttattgtag caatttgaat
     2101 attgcatata aagttattca ttttccataa aagtcgtcta tattgacgac tgattatgaa
     2161 agttatataa ctttctaatc actacttgag ctgtccaaaa gaattttaat attctccaga
     2221 cagctttttt ttatgatatt tgattgatat acatgtctag ttattccata tttagtaaat
     2281 attttgataa aatttccaca ttcacttata acttaggata acgttttttt caaacaatac
     2341 attaatttaa aagtattctg agtatacatt atttagtatt ttaattaaat ttagcagttg
     2401 acttttatta ttgtataatt tataatttat aacaagtcga gaatacatat aattttctga
     2461 ctaattaaaa tattaaaagg gagagataat tatgaatact ttattaaaca atagaaaata
     2521 ttattattat tatagccact atttttaggg tttgtaccta cgttttaaca tagatacaga
     2581 ccccttaaga ctactaaaat tttgggattg tatctatgtt ggctatattg tttataaagt
     2641 tttcatttaa ctatataaga gccacataga ttaaactatg tggcttattc gtgtttttac
     2701 cacatagttt tgtacactat gtggtttttt tatacacaaa attagcaaat aattaaaatt
     2761 ataaaaatat tttcaaagga gtgttttatt tatgtcaggt attatatctg taatgacgca
     2821 aagtttaatt ttatcgatta tggcactggg ggtttatata acttacaaaa tcttagattt
     2881 tccagatatg tctgctgatg gtagctacac aatgggtgct tctattgtag cattttctct
     2941 tactaatggt atttctcctg tagtagcaac tttaatggct atcttatgtg gttgtactgc
     3001 tggtcttgtt acagggattc ttcacattaa gtttaagata tctaacttac tttctggtat
     3061 tttagttatg ggaatgctat actctatcaa tttaagaata atgggaaaat caaatattcc
     3121 tctatttagc tttaaacact tattcaatgg ggaaatatct cctatagttt tagccttagc
     3181 ttttgtattc atctgtaaag tccttttaga tttattccta aaaacaggtc ttggatacac
     3241 tttaaaaggt gttggtgata actctcaaat gattaaatcc ctaggtataa atatcggttc
     3301 tataaaaata ctaggactta tgatttcaaa
//
LOCUS       DAMJWU010000200         3312 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMJWU010000200 DAMJWU010000000
VERSION     DAMJWU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.206"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(22..387)
                     /locus_tag="PC460_003659"
                     /pseudo
     CDS             complement(22..387)
                     /locus_tag="PC460_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(408..1487)
                     /locus_tag="PC460_003660"
     CDS             complement(408..1487)
                     /locus_tag="PC460_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF2602503.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            complement(1541..2830)
                     /locus_tag="PC460_003661"
     CDS             complement(1541..2830)
                     /locus_tag="PC460_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2602504.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     misc_binding    complement(2993..3204)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1093 a          662 c          364 g         1193 t
ORIGIN      
        1 agtgtgcgta gctatttttt aatcaaactc atatccacca gataccaata ttgaatctaa
       61 ttctattttt tctcttaagt cttctggcca atctataaat ggaagaatgg cagtagtggc
      121 tggctttcct attcttatca tagccacatt attaagtctt tttctttcta caatagcatt
      181 tgtacaatga gttgtaccaa gcatagcata ttttattaat gaatggtcaa tcttactttc
      241 atctataact ttttttaagg cattgtaaac accagtttca acatcttttg tagttgccac
      301 cttaactgaa tgaattactt ttaattcccc atccagtata caagcatctg tatttgttcc
      361 tccaacgtca attcctacac gatacatcaa aatttcctcc taaactttta ttttccttgt
      421 aattcttcta ctgttttata ttccacatca tatccaaagt atccaggtcc aactgtctca
      481 attccttttt ctgttctcca tttttcatta caaggaaaag ctactaccat aacacgagag
      541 ccatatttta ttctctctgt agttattgga aatccagttt ctaaatctaa tactgctatt
      601 aaatctggtg taatacaaag tggtgtatca ccttttttgg caattaaatt ctcattttgg
      661 aataatatag aatactctcc atcatactca ttaatcccag caaaaaaggc atgtcctcta
      721 gtaaatccac ctttcaactc tctcttaata tcaattactt ttccttcaaa taacttgtat
      781 ccacgcaatt cttttagtat attctcaaca gcaccttttg aatcagattt attttcaagt
      841 aaaaaccctc ctatagtttc agctattgta agagtattac ctatagcagc ttttttaact
      901 tgagctccac ttaaattata gtcactaata gaagaacttc ctcccatttc tacagttaaa
      961 gttctagcta atctttctga ccaaattcca tccacaggat aaaggatggc agaattacct
     1021 ttttcatcag acattacggc aggagatgcc tcatatcctt caagataaaa agttaccatt
     1081 tgagcttcag ggaaagctct acccatagaa tctgcatcta agataggaag tcctttttta
     1141 gcagcaactg ctataggcat aagtgagttt atccctccta tttctatagg aattataaca
     1201 tccacttttc tatttaataa tttctccatt atttctaaag gggtagttaa ctcaacttct
     1261 gaaagtaatt tttctattgt aattacagga gaacccatac cagatactgg aactaccaaa
     1321 gcatcatcat caagttcatc taaacttatt acttttacag gaccatactt tttaatggct
     1381 tgttttgcaa ccaatttacc tacatatggg tcacctccac ctccagttcc aagcactgct
     1441 gcaccaactg ctattttatc tacactttct tctgttaaaa atctcatgtt actttctcct
     1501 catatttcta atttatttta tactttataa acaaatttct ttatactgct ttgtctattt
     1561 cttgcttatt ttttgttgaa caaagcactt ttccaataat agattgtact acaaaccctg
     1621 ctataaaacc atctaaaggt gctatagtag taagagttat aaatccatat gttgaaaggt
     1681 aagttattaa tataccaatt atccaagaca ctattgaacg tttaactatt ggtttatata
     1741 atactccttt atcaaagcct ttaagttctt gctttactat gtaatattct gctgtatata
     1801 caccaccaac aggtgcaatt acaattccaa gtaaatttaa aaatcctata aaaccttcat
     1861 atattccaaa tacagccaat aatgttgcta taataccaag gactatagtt aatactttct
     1921 caggagcctt cttaagtaca agagctatac ttaatgatgc tgaatataca ttacttgtat
     1981 ttgtagtcca ttgtgctaat gtaagtacta ttatacctgg tatagcaatt cctaatgtaa
     2041 taaaaattct cataatatct gaagttccca tacattttac taatactatc gaaactatta
     2101 tcataaaagc atttccaaat tgtattccca aaaagcttga aagagcacag tctcttctac
     2161 ttttagccca acgacttata tcaggagata caattgcacc aactattatt atactcattg
     2221 acagagaaac tccacctgca aatgtcatag tagatttcat attatcatca actgaaattc
     2281 catttgctct aaaagctaag aatatagtta gcatcataag aactaataaa aatggtactg
     2341 aatacacact caacttctcc attgccttat atccataaat tgcagtactc atcatcaaga
     2401 gaccacctat taggcttaaa atctgcatac ttacatctaa attaaatata tcttttatta
     2461 ttatctgtgc attttgagca aagaaaccta cttgaactcc aaaccaacct aatagagaaa
     2521 cccctataac tagagaaaat atttttgctc cagcttctcc aaatacatac tttgttatca
     2581 tagatgtaga taaacttgta gatgagccaa ccaaagagca tgtagtatac aaaattgcag
     2641 aaaaaaatga accaacaatt actgacaata ttgcttgttt cattggcata ctctgtgcca
     2701 actcagcgcc taaaataaca gatgataaat ccacacctat tgctactaaa actgaaaaca
     2761 tagctaccca accacgtttt tgattctctg gaatctgttc cagagcatag ttaatttttt
     2821 cttcttccat tttcatccct ccatttaaat cgtaaaacta ctgaccttag catattttac
     2881 aattttctaa tttataaatt tattaactat catattttta taattttcat ttaatatata
     2941 gttttaaatt ttctgaaata agtttataat aaaaaacgcc tcttggaaaa atccaagaga
     3001 cgaatataaa tccgtgttac cactcttatt agtaaaaatt aattttactc actcattttc
     3061 ttaaggcgaa ttaaaccact attctaacat aattacaata gtattctcca aagtcctttt
     3121 cacataaatt ccaatattag ttctcagcca aatctaactc tctgtaaaat tcctttatgc
     3181 tactttcttc ttctacgaat atataatatt gatttgtagt taatttatga tttatatcat
     3241 aatatatatg caatattttg tcaacttaaa aagtcaatta cttgcatttt ttagttttat
     3301 atgtaaattt tc
//
LOCUS       DAMJWU010000201         3275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMJWU010000201 DAMJWU010000000
VERSION     DAMJWU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.207"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            438..1052
                     /locus_tag="PC460_003662"
     CDS             438..1052
                     /locus_tag="PC460_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2602505.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTNRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            1070..1432
                     /locus_tag="PC460_003663"
     CDS             1070..1432
                     /locus_tag="PC460_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602506.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1538..2035
                     /locus_tag="PC460_003664"
     CDS             1538..2035
                     /locus_tag="PC460_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2602507.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            2839..>3275
                     /locus_tag="PC460_003665"
     CDS             2839..>3275
                     /locus_tag="PC460_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2602508.1"
                     /translation="MIAVKKAYKFRIYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKG"
BASE COUNT         1298 a          373 c          517 g         1087 t
ORIGIN      
        1 ttgtcatact aacttcaaac aaattatgat gttttataaa ttatttgtaa atatcattat
       61 aattacttaa gttattcttt atcataattg cttaaattac tctttatcac ctaattatac
      121 accatcaaat aggattggta aaatattcat ctcatataag tattactata taataattta
      181 ttctattagt atactcaaat catgtaaaat ttatataact caattaattt tttaaataca
      241 actttataat ataaaactta taataaaagt aaatttatat gttaaatgag gttgattttt
      301 tattattttt tatttatgct agtactaaag acttgcaaac gttttttgac aagtctttat
      361 tttgtctaaa ttaaacaaac ttttttacaa ataaatacat aaaatttaaa ctaatctatt
      421 tattggagga gataaaattg gaagaagtaa ttgcaaaaat tattactata gaaaatggat
      481 tcaaagaaat tgaaaacatt gctaaaaaaa ttgtaaaaaa taatgactca aaaaagtgtt
      541 attatctatc aatctactta tataggtcag aatattatca agtaagagaa ttggctgtgt
      601 ttatacttgg ttttatatca gtaactattt ctgaagcttt gttattttta aaagataatg
      661 tgagtaaaga tgaaaactgg agagtacaag aaatactagc taaatcattt gattattatt
      721 gcagtgaagt aggatatgaa aaggcacttc ctgttataaa agaatggctt aattctgata
      781 gtccaaacat aaaaagggct gtgacagaag gtcttcgaat ttggactaat agagactatt
      841 ttaaatctaa tcctgacgtt gctatatcac tactttctag tttaaaagaa gatgatagcg
      901 aatacttgag aaagtctgta ggtaatgctc ttagagatat aagtaaaaaa tatccagatt
      961 tagtaaaaaa agaattagat tcatgggata tatcaaataa aaaagttgag tttgtatata
     1021 agcttgcttc aaaatgtatc ttggcaaagt aaaatggagg tgaacttatt tgaaacttcg
     1081 tgatgagtat acttgcccat tagaactaac acatgatatg ataaaaggca aatggaaatc
     1141 tattatatta tggagattac gcataggacc aacttcctta tcaaaacttg aaaaggaaat
     1201 caatggtatt aatcaaaaga tgttacttga acaacttaaa gaattaattg aatttggtct
     1261 tgtagataaa aagacatatg aaggttaccc acttaaagtc gaatactcac ttacacctat
     1321 tcgagggaaa caaatattgg atgctttggc tataatgcaa gatataggaa ttagctttat
     1381 gttggataat ggtatggaag atattcttaa ggaaaaaggt gttttaaaat aaatagatac
     1441 ccacaaaaaa gtaagtaatt taccattatt ttcaactggt atataattaa attatagata
     1501 tattctatta atttattata ataggaggta aattattatg aaagtaacta gtacaggtat
     1561 agtaaatggt gttatagaag ataaatatgg taaaagagga actcagttca atgaaggtgg
     1621 tatgcctaca tattcactac ctctaaagtt tgaagatgct ccaaagaata cagtatcctt
     1681 tgctgtattt ttagaagata aagactctgt tcctgtatgt gggtttgcat ggatacattg
     1741 gttagctgta aatataacaa aagatgagtt aaatgaaaat gaaagtgtaa cagctactga
     1801 ttttgtacaa gggactacta gttggcatgg taaactagga ggtcttgaca gattagaggc
     1861 cagtgtatat ggtggaatgg ctcctccaga cagacctcat gtctatgaaa tacatgtatt
     1921 tgcacttgat actatccttg accttgaaaa aggattctat atgaatgaat ttttcaaagc
     1981 tattgaaggg catgttttag atacatttac actaaaagga agttattcta attaattagg
     2041 ttgactatag ctaatcatta attaaatgat acatttaatt gatgattagc ttaattgtta
     2101 ttagaatatt ttctgcactt tatcttaata tatattttag ttgactggta ttttaatttc
     2161 tattagatta ttatcaattt atataaattg attaaaaaat agctacgcac actcgtgact
     2221 ttagtcatga gttagtagct aaaatagtca gcatataggg aaacttatat gtagagatag
     2281 gatagaacct atccaatact acttgaattg ctaaaaaccc ctaaagctaa ccaaactaaa
     2341 acataaggat gaaataaacc taagtgtgaa agttacgaaa gtagaaaaaa ttggttagat
     2401 ggtataaggt taaaccctaa gtactttaaa atgggcaatc agcaggtaaa ctccaaatag
     2461 gagaaacttc aacgactatt cctcttgagg gaagtacact acaagttatc ggtagtggaa
     2521 gtgggtagac cttagcagat aatgccgaag gataagatat agtctgtgct tatgtgaaag
     2581 tgtaagaagt tcataagaga actgcatagg tagtagcgaa cttatgtgaa cgacaacctc
     2641 aaaaatgata aaagttaaga cttacggttc ttatatatgt tatttaaaat taaatataaa
     2701 accttctaaa taattgtttt aaataattac atttgttaac attattatag gtgattaata
     2761 aattgttcac cacagcatta tataatatat taaatattaa aatcattaaa aagtgtatca
     2821 aaaaacaatt aaagtgaggt gatagcagtg aaaaaagctt ataagtttag aatatatcct
     2881 aataaaaaac agcaagagtt aattaacaaa acttttggat gttgtaggtt tgtatataat
     2941 aaatatcttg ctaaaagaat agatgtttat aaaaacgata aagaaacttt tacttataaa
     3001 cagtgtagtt ctgatttaac caatttaaaa aaagaattaa attggcttaa agaacctgat
     3061 aaattttcac tacaaaacgc cttaaaagat ttagataatg catataaaaa attctttaaa
     3121 gaaaaggcag gatttcctaa atttaaatca aagaaaatta atagattttc atataaaact
     3181 aactttacaa atggaaacat tatgtattgt ggtcaacata taaaactacc taaacttggt
     3241 atggttaagg taagggataa acaagtacct aaagg
//
LOCUS       DAMJWU010000202         3156 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMJWU010000202 DAMJWU010000000
VERSION     DAMJWU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.208"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            170..964
                     /locus_tag="PC460_003666"
     CDS             170..964
                     /locus_tag="PC460_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602509.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            965..3025
                     /locus_tag="PC460_003667"
     CDS             965..3025
                     /locus_tag="PC460_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2602510.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1210 a          323 c          512 g         1111 t
ORIGIN      
        1 aaaatgttac aaaagatatt ataaacgaat aaaaaataat aatttgccag ataaacttgt
       61 aaatttttat gaataatcca ttctaaaaca aaaaactgtg tataaaaaag gtttatatgt
      121 tatatttata taaatataac atatgaacga gaaaggagtg gtttgataaa tgattgaggt
      181 agataaattg tgtaagtctt ttacaagggt tgtaaaagat gataagaata aaagttcaat
      241 aagaaagcta aagaaagtaa aaactaaaaa agaagaattt tttgctgtaa ataatgtttc
      301 atttaaagtt atggaaggtg aaattgttgg aatattaggt ccaaatggtg caggaaagac
      361 tacattactt agaatgttag gtggcatatt aacaccaaca tcaggaagta taaatatttc
      421 tggatatgat tattcaatag atagaaattc agcaaaaaaa gaaataggtt atctctctgg
      481 aaatacaaaa ctatatggaa ggctttctcc gagagaactt ctaacaactt ttgcaagtct
      541 atatgagatg tcaaaagaag atatagaaga atcaattgaa aatattgtaa aaataatgga
      601 tatgagtgaa tttatagata atagaattga aaatctatca actggtcaga ctcaaagaac
      661 atctatagct cgttgtttaa tacattctcc aaaagtatat atttttgatg agccaacact
      721 aggtctggat gttttaagta gtgcatctat tatagatttt atgaagaatg aaaaactaag
      781 gggaaaaact gttctttact caactcatta tatggaagaa gcagaaactt tatgtgacaa
      841 gatttttatg attcacaatg ggaaaataat tgcatcagga acaccacaga gtttaaaaga
      901 ggaatctgga gtaaataatt tgagagatgt attcataaaa cttgccagaa aggaggacat
      961 ttaaatgaga agtaagattg taaaatacat atttaaaaaa gaaatgttag acatattgag
     1021 agataaaaag actttattta tgatgattgt tgtgccactt cttttatatc ctttacttat
     1081 gttattaatg attcaagtaa tgaatatgtc tgcaagtagt atgaatagta aggacattaa
     1141 tatagctttt aataataaac ctaataaggt tttagtatcc atgattgaag atgataattt
     1201 agaaaaagat actaaaaata cttcaaatga taaaggcaaa ataagagtgt tagatgtaga
     1261 taactataaa aagtcattag aagaaaataa aattgatgct tatataaaga tggaagaaaa
     1321 aaaatcatct ataaattatc aaatatatat aaattcttct caagaaaatt cttctaatgc
     1381 actaaaaaga attgaaactg ttttggataa atataaaaga tttaccatag aaaaaacttt
     1441 atctgaaaaa ggattagatg tacaagctac tttagaacca ataacatata gcactgtaga
     1501 tgttgcaaag acggaagaag tggcaggata ctttttaggt cagatacttc catttatatt
     1561 aataatagga gttttactag gttcaatata tccagcaata gatgttatgg ctggagaaaa
     1621 ggaaagaggt acattagaaa cattatttac actgccaata tcaaacttag aactggttat
     1681 gggaaaatat atggcagttt ctcttagtgc aatagtaaca gcaattttaa acattttatc
     1741 tatgggactg actatggcat ttattttagt tagtggaggt attagtagcc aatttggctt
     1801 tggaaacttt aattatggag acttagtctt accaattata actactttaa tttgcatatg
     1861 tcttttttca atggtagttt cagcaataag tatgtgtgta tgttcattag caaaaagttt
     1921 taaagatgct caaaactaca taacacctct tatgttaatt attatgatac catcatatgt
     1981 ttctatgata ccaaatatag tattagatag agtgacagct gtaatacctg tagtaaatat
     2041 atctttactt ataaaaagtg tattatcttt taaaagcgat ttaaatttaa tagccttagt
     2101 tttagcatca aatgtagcct ttgttatatt ggcaatcata ctactgtcta agatgttcaa
     2161 ttctgaggaa atactttttg gtaatagtaa gagtttctct ttcttagaga aaagaagtaa
     2221 tataaaaaaa gggtctatac caactgtaag tgatggtgtt attttatatg cagtaggatt
     2281 agttctattg atttatgttg gttctttagt tcaaattaaa tttggtatgg caggtattat
     2341 aatgacacaa ttcatgataa tttctttacc gttattattt gcatactata ttaaagctga
     2401 ttttaaagag gtatttagct tgaaagttcc caaattaaag catgtgtttg ggagcattgt
     2461 tctttggatt ggtggataca tacttattat gattataaca cagctaattt tattcttatt
     2521 tcctcaaaat atggaagttg ctgaaagttt aaataaggct ctttttatca aagatagtgt
     2581 tttactgaat ttactagcaa tagctttatt accagcttta tgtgaagaaa tgttttttag
     2641 gggatttata ttttcttcat ttagtaaatc aaaagataaa aataagtcta tcaagctagc
     2701 tataatatgc agtggggtac tatttggtat tatgcatatg gattttatta gaataattcc
     2761 aacatcaata ttaggtatta tatttgcata tagtgtgtat aaatctggtt caatatttgt
     2821 atcaatgtta ttgcattttt taaataatag tgtagcagtt gtgttaaatc attatacaac
     2881 tggaaatata accaacttat ataaatttgt tgaagttgat ttttcgaact taaatgtact
     2941 taaatttgcc ttaattcttc taataagtct tatattaatt atgttaggat taagagtttt
     3001 agatagaaag tctttaagaa attaagtata aagttattat atagattaaa atattaataa
     3061 gcactaaagt atatggatat atcttgtata taaaaatact ttagtgctat tttatatatt
     3121 ttaattatat gaattattta tcgtttcatt tatgta
//
LOCUS       DAMJWU010000203         3027 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMJWU010000203 DAMJWU010000000
VERSION     DAMJWU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.209"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            172..1065
                     /locus_tag="PC460_003668"
     CDS             172..1065
                     /locus_tag="PC460_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2602511.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            1502..1993
                     /locus_tag="PC460_003669"
     CDS             1502..1993
                     /locus_tag="PC460_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602512.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            2021..2968
                     /locus_tag="PC460_003670"
     CDS             2021..2968
                     /locus_tag="PC460_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602513.1"
                     /translation="MRITKTTFNDVFSSDANESIIKVEKYVDKKCASVFEILTYTIIV
                     TNTNRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         1323 a          265 c          426 g         1013 t
ORIGIN      
        1 atttatttta actattttgt gtgatagaaa actatatatt ataacaatag tagtgctttt
       61 aattaaatat atttcataaa tcttcataaa atcttcaaaa tttcgtaaag aatatgtcac
      121 aaaagtaagg tatattatat tcagcagata gtataaggga ggacaatatt tatgaagaat
      181 aaaaaattca ttgttataat actgataata tcaattttaa taggtgtatt agtgaaggaa
      241 tattcatcta gagagataaa aaaggatgat ataaatgtca gtaagtatat aaaatatgct
      301 gatttagcaa gtaaaaataa tgcacaagtt aactggaagt atgtagcttc cattgttgct
      361 gtgttaaata aaaataactt aaaaaacgtt aaggattcac agatacaaga agtatcagat
      421 ttatttgtta agaatttttc taaaaataat aaaataaata agttaagtga tattttggat
      481 gagttagaat ttagtaatag acaaaaaaga ttggttgata attatataga caatcttaag
      541 gattatggta taaaaccaga aagattgaag tctgatacaa aatatatgaa gtttattgat
      601 gaaattaaaa cagaagcaat acaaaattat aaggattata aaatacttcc atctataact
      661 attgcacaag ctataataga atctagctgg ggcaagtcaa ctttggcaaa acaatatagc
      721 aatctatttg gtataaaggc tgacgcttat tggaaaggta agtcagtgac tttagaaacg
      781 aaagaacatt tagatacaat tatagatgat aaatttagaa tatatgatga taaaaatgaa
      841 tctataaaag accatgcaaa atttttagcc acaaataaaa ggtataaaaa caatggtgta
      901 tttgatgcaa aaacgtatat atatcaagct aaagcattag aaaaggcagg ttatagtact
      961 gcgaaggatg aaaatggcaa tagtatatat gcagctcgtc taatagaatt aattcaacag
     1021 tataatcttc aattaataga cagtgaaatt caaagtgaaa tgtaactttg attaaatttt
     1081 tattgataag gctatgattt ttaaaatcat agccttattt tgacatgctt ttacttgtaa
     1141 ttattaaatc gatatcatta tctttcaaaa tttactttat actgtaaatc aaattattta
     1201 agttaaaact tggaattatt tataaaagga ttatttactt tatctaaatt ctgcacttaa
     1261 agaacatctt ttcccttaag actgaccgtt agtctataaa aattataatt acttataaaa
     1321 aagtgaatat atatataaag ttttcagaaa gacttttact tgtaaattga aatcaatgaa
     1381 gtaaaaaagg attctttctt aaaagtaaat aacaactaga tagtgaataa taaaaaatgg
     1441 taatttgaaa taatttttat gaatagtatt aatatataga tacagggaga ttgatgcgat
     1501 tatgtatgaa aataataagg taatatacaa ccttgaaaga tgtgaaaaac tagaaaagac
     1561 tctttattta tctcaattta atacatataa aaattttaaa ttagcttcta aaaaaactgt
     1621 aataaattca ttagagacat atgtttacaa tatgaatatt aacaatataa gtcttgttga
     1681 tactatagaa tgtatatcat tagaagatga acaacttaca ggaaagaagt tgattttagt
     1741 tggtgatata gatattgatg caatattaga ttatgcagga aataaaagaa atagaaattc
     1801 tagtaaaaaa aatacattta aattaaaaat accctttagt acatttatac aaatgcctag
     1861 aaagatagaa aataaagata aaattaattt aaagtatctt atacaagata ttacttcatc
     1921 tattttagat gataatttat ttattagtgt tactgcaatt ataagttatg aaaatagttc
     1981 caagatagag taatctttaa attatagaaa ggtggatgca atgagaataa cgaaaactac
     2041 ttttaatgat gtattttcaa gtgatgcaaa tgaaagtatt ataaaagtag aaaaatatgt
     2101 tgataaaaaa tgtgcatctg tatttgagat tttgacctac actattattg ttacaaatac
     2161 aaataggtac aaaactggaa atatattttt caaagattat atttctaaat atatagagtt
     2221 tataaataat actgtaaaag ttaatggcat tataaaacgt ggattagacc cacagcaagg
     2281 tttttacata gggagaatag atgcaagttg taaaaaaata atatctttta agagtgtagt
     2341 tttaccaaat tctgcacata ggattataaa gaatagtgct aatatatact attattataa
     2401 gtgtaatcta gataaattcc caacaagaat atcaatagaa agtaatagag tttatactaa
     2461 tgtaaataaa attgtattca aacaattgaa tataagtaat actttaaaag ctcctaaaaa
     2521 tctaagagat atattaaaag taaatgtaaa tagtaaaata tttgatgtta aacctatgaa
     2581 aaatcttata aacaaaggta gtaatttgaa attatgtaac ttgatagttt ttgggtgtat
     2641 agaagttgaa atagactata gttgtaaaaa taggagtaat tttaaaacag taaataagtc
     2701 taatgacatt aaaaaagata agtattataa aaatgatata caaaataaca atgaaaatat
     2761 aaacgattgt aaaaagcaaa taagttttgg tgataataag gtaaaaaaaa taacaaaaac
     2821 ttttggattt tcttgtttca tttgttctcc agttggaatt atttatgaag atatgaaaaa
     2881 tataaatata aaaattgaac atacatcaat aaatgaatta aatccagaag aattatttgt
     2941 aaatacatca cttttattgt attattaagt attttaaagc taaaacttaa acttattaaa
     3001 atagcatttg gttaaaggaa aatagat
//
LOCUS       DAMJWU010000204         2991 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMJWU010000204 DAMJWU010000000
VERSION     DAMJWU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.210"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            375..1652
                     /gene="hflX"
                     /locus_tag="PC460_003671"
     CDS             375..1652
                     /gene="hflX"
                     /locus_tag="PC460_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2602514.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVDVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     MSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1885..2382
                     /locus_tag="PC460_003672"
     CDS             1885..2382
                     /locus_tag="PC460_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2602515.1"
                     /translation="MDAETLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2432..2956
                     /locus_tag="PC460_003673"
     CDS             2432..2956
                     /locus_tag="PC460_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2602516.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         1242 a          262 c          527 g          960 t
ORIGIN      
        1 tgcaaataac tacttaaatt taagaatagg gttaaatata aaatgataat tacatataag
       61 ttaattaaac taatgtattt ttaatatata tttagaattt taatccttct tatgatataa
      121 gtagcttgtt ttgtattact tagatttgta aggagttttt ttattgaaaa aactgcataa
      181 tatatttggg aggaggtgta agaaagtgat tttttatata agattgagaa aataatgcac
      241 gattatggtt attataatag taaataagaa gtgtcattta aggataagat aaaaagcaaa
      301 attcttgatg aatttaatta tagagccata atctcgtgct atagattaaa taaaaaatag
      361 cagataagga gaatatggat atggcgaaaa aaggaataac agtaggatta aacataaata
      421 ataaatctga ggactttaat gaactaatga ttgaacttga aaacttatgt tcagcatgtg
      481 atattgatgt tgtagggagt ataactcaaa atgcaaagca ggttaataga gcattttaca
      541 ttgggacagg taaagttgag gaaatattaa acttaataaa gaaagaaaat atagagatag
      601 ttatatttta caatgaactt tctacatcac aactaaaaaa tcttgaagaa aagcttaatt
      661 gtgaaattat agatagaacg gcattgatac ttgatatatt tgcacaaagg gcaaaaacta
      721 gagaagctaa acttcaagta gaagtagcaa gtttaaaata tatgttacca agacttatag
      781 gttcaaatga aaatttagga cgtcaaagtg gtggagttgg tactaaaaat agaggttctg
      841 gtgagaaaaa attagaactt gaccgtagaa gaatagaaga aaagataaca tctttaaata
      901 gggagttgga tgatttaaaa ttccaaagag aaactcaaag aagtatgaga agaaaatcaa
      961 atcttccaaa tgtagctcta gtcgggtata caaatgctgg taaatcttca ataatgaata
     1021 aactagtaga tatatttaaa aactctgaag aaaagaaagt gtttgagaaa aacatgttat
     1081 ttgctacact tgaaacttct gtcagaaata tagttcttgc aaataacaaa gaatttttat
     1141 tatctgatac tgttggtttt gtaagtaatc taccacatga cttagtaaaa gcatttaggt
     1201 caacattaga agaagcatgt gaggcagacg tactgttaca tgttattgat atttcaaatc
     1261 caagctataa aagtcatatt aaagtgacag aagatacttt aaaacaaata ggtgcagatg
     1321 gtatacctat gatacatgtg tataataaaa ttgatttaat agatgtagac gtattggata
     1381 gaattttaga tagtattgat aaagaaggta tatttgtttc agttaaaaaa gatataaata
     1441 tagataaaat gataaaatgt atttgtgata gcatatttaa agattatgta agatgtaagt
     1501 tcttaattcc atatgataaa ggtcatgtag tttcttattt taacgaaaat atgagcatta
     1561 taaatacaga atacagagaa gatggagcta ttttagatgt agaatgtagc aatatagaat
     1621 ataataagta taaaaagtat gctttagagt aaattttgaa aaacttatta tagcctaaaa
     1681 tatatttaat ttataattta ttataaattg attgtaaata tgattttatg aaacggtata
     1741 gccataatgc tatgccgttt tgccatttat aataaaaata aatttattga cataatagta
     1801 actaattact ataatataaa gtaattagtt actatttaaa attatcaaaa aattaaaatc
     1861 taagataaag agaagtgtat aataatggat gcagaaactt taatgaaaaa atatatggta
     1921 gatgatgata gtagaaaaca agctatattt gcaagtatat ttgtaatgca aaataaactt
     1981 caaactacat gtgataaatt agacccagaa ttaacaatga aacaatggtt acttttagct
     2041 attgcggact ctgtagatga aactcttaca ttaaccaaac ttggagaatt aatggggtgc
     2101 tcaagacaaa atgtaaaaaa actagctatt gctttagaaa agaaaggttt tatacaaatt
     2161 gagcagaatg aaaaagattc aagagctatc tgtcttgtga caaaagatag agtgcaagag
     2221 tattctaaaa aagtaagagg tatgcaggaa aaagtattac agcttttatt tgaagatttt
     2281 acagagaaag aagtggaaca gttatttttt ggaattatga aattaagtat aggaattgaa
     2341 aatgtgaaaa attatgtaga aaataatgac gttggagaat aagcaagtat caaatcaact
     2401 ttgtatgaca tcagagaggg ggataattta tatgaaaaat gtagtagtga tttataagag
     2461 taagtatggt tcaacaaaaa aatatgctaa atggctttca gaattattgt cttgtgatat
     2521 ttttgaaact aagaatatta atatagaaaa ggttatgaag tatgatacga ttattttagg
     2581 aggaggtctt tatgcttgta atatagcggg aatttctttc ttaaaaaaaa attataaaga
     2641 attgcagaaa aaaaatttag tagtatttgg cgtaggagct gctccatatg atgatgaagt
     2701 aataaagaaa ttaagagaag taaattttaa ggatgaatta aacaaaattc cttgctttta
     2761 ttgtcgtggg gcatgggatg agacaaaaat gtcttttata gattatactc tttgtagttt
     2821 ttttaaaaaa tctatagcta aaaaagaacg tcaaggtctt aaaccagcag aagcaccttt
     2881 attgtctgga gaagcatttg attggtgtaa taaagaaaat ttaactccaa tacttgatta
     2941 tataagagaa ctttaagagg tatatattta taattattta ttatctaaag t
//
LOCUS       DAMJWU010000205         2902 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMJWU010000205 DAMJWU010000000
VERSION     DAMJWU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.211"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..438
                     /locus_tag="PC460_003674"
     CDS             <1..438
                     /locus_tag="PC460_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2602517.1"
                     /translation="EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKF
                     KKAIRELLWFVCEYLKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDET
                     IVSNHPWVEDVNDELERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            428..1921
                     /locus_tag="PC460_003675"
     CDS             428..1921
                     /locus_tag="PC460_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2602518.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWAIDGLNFSERIWGKYRPTLVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLEVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKNNSKQTGAKYTKGDIEWNIRREEEAELYYDNIRNRKDDISKISKNTNWSE
                     KSIGQIKNHIFYNTHIMRDGTRRMLDSDYSMSVAWQRLINGTYEDIDILLLKHEYLES
                     IFEKKYNISNLEAHRMTEKKHDWYKELIKQKGEFEEDDCLNELIRKE"
     gene            1887..2129
                     /locus_tag="PC460_003676"
     CDS             1887..2129
                     /locus_tag="PC460_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602519.1"
                     /translation="MIVLMNLLEKNNEYVIYSYGYEENKLDGRIKIYLDDFYNYEIIK
                     ESKDEHISKSATLKAISKLIKAAKNNDLKKEMSYQC"
     gene            2200..2394
                     /locus_tag="PC460_003677"
     CDS             2200..2394
                     /locus_tag="PC460_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602520.1"
                     /translation="MYILTQVIAVVCVVQIFINCIANVNVGILCNKLKEKNEANIDKV
                     SDEILKRVGEELNESLDKSL"
     gene            2439..>2902
                     /locus_tag="PC460_003678"
     CDS             2439..>2902
                     /locus_tag="PC460_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2602521.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGE"
BASE COUNT         1259 a          274 c          526 g          843 t
ORIGIN      
        1 gaaaagaata taattatctt tggtcaagga gttaatccag aatctcaaaa cacaggtgac
       61 aaatcgggtg tagcacttaa atttttatat tcactacttg acttaaaatg ttctaagact
      121 gaaaagaagt ttaaaaaagc aattagagag cttttatggt ttgtgtgtga gtatttaaag
      181 ataagtggta gtaagagcta tgattataaa acagttcaaa ttacttttaa tcactctatg
      241 ataataaatg aagctgaaaa gatagatatg gcagctaaat caactggaat tgtatcagat
      301 gaaactattg tttctaacca tccttgggtc gaggatgtta atgacgaact tgagagactt
      361 aaaaaacagg aagatactca aaaagagtat gatgatttaa ttcctaataa tcaagatggt
      421 gttatagatg aaacataaag attattggag aaagagattt gaacaattag aagaagctca
      481 aaataacaaa agtgtaaaat attatcttga attagaaaag caatataaac tagctataaa
      541 tagtatagaa aaagatatat tagcatggta caacagattt gccaaaaatg aaggaatatc
      601 tttattagaa gctaagaaac tactaaatac aagagaacta gaagagttta aatggagtgt
      661 tgaagaatat attaaatatg gtaaagaaaa tgctataaat caaaagtgga tgaaagagtt
      721 agaaaatgct agtgcaagag ttcatataac aaggcttgaa gctttaaagt tacaaataca
      781 gcaacaagta gaagttttat atggaaatga acttgatggt attgataaac taatgagaga
      841 tatttataca agtggatact atcatacagc ttttaatgtt caacaaggag taaacgttgg
      901 ttggagttta atgagtcttg atactaatag aataaataaa attatctcta aaccatgggc
      961 aatagatgga ttaaacttta gtgaaagaat ttggggtaag tatagaccta ctttagtaaa
     1021 tgaactacat actaagctaa ctcaatcaat tattagaggt gaaaatccaa aaaatttagt
     1081 aaatgacttt gctaagagat ttaatgtatc taaatcacaa gctaagaatt tgataatgac
     1141 tgaatcagct ttctttgcat cagcaagtag aaaagattgt tttagtgatt tagaagtaga
     1201 gaaatatgag attattgcta cattagattt aagaacttca aatatatgca gagagttaga
     1261 tggaaaaata tttgatatga aagattatca agttggaata acagctccac catttcattg
     1321 tcgttgtagg acaacaacag ctccttggtt cgaggatgaa gaaggctata gagcagcaag
     1381 aggagaagat ggaaaaacat attatgtacc atctagtatg aagtataatg agtggtatga
     1441 gaagtatgtt aaaaataata gtaaacaaac tggtgcaaaa tatactaaag gtgatatcga
     1501 gtggaatata agaagagaag aagaagcaga actatattac gataatatta gaaatagaaa
     1561 agatgatatt tccaaaatat caaagaatac aaattggtca gaaaaaagta taggtcaaat
     1621 taaaaatcat attttctaca atactcatat aatgagagat ggaactagac gtatgttgga
     1681 ttctgactat agtatgtcag ttgcttggca aagacttata aatggtacat acgaagatat
     1741 tgatattctc ttattaaaac atgaatacct tgaaagtata tttgagaaaa agtataatat
     1801 aagtaactta gaagcccata gaatgactga gaaaaagcat gattggtata aagaattaat
     1861 taaacagaaa ggagagtttg aagaagatga ttgtcttaat gaacttatta gaaaagaata
     1921 atgaatatgt tatatatagt tatggatatg aagaaaataa gcttgatgga agaataaaaa
     1981 tatatttaga tgatttttat aattatgaaa taataaaaga gtcaaaagat gaacatataa
     2041 gtaaatcagc aacgttaaaa gctatttcta aacttataaa agctgctaaa aataacgatt
     2101 tgaaaaaaga aatgagttat caatgttaga agcacttact gaacaataaa ttagtaggtg
     2161 cttttattat gtaaaagttt aaaaaagtag gtgattttaa tgtatatatt aactcaagtt
     2221 atagctgtag tttgtgtagt acaaatcttt attaattgta ttgctaatgt caatgtaggt
     2281 attctttgca ataaattaaa agaaaaaaat gaagctaata tagataaagt ttctgatgaa
     2341 attctaaaga gagtaggaga agaattaaat gaatcactag acaaaagtct ttaaagactt
     2401 tttttattgt gtaaaaaatg aaaggagata tttaaaagat ggattggtta aaagaattgc
     2461 tagaaggaat aaaaatagaa aataacaaaa ttgatgtagt ttctcttcaa aaatctatag
     2521 aaaagaaaat aaaagagact acaattactc aagaagatta tacaaatctt gaaacacagc
     2581 ttaatacagc taatgaaact attaaaaagt ttgaaggagg tatgacaaaa gaagatgtag
     2641 agaatctaaa aacaacttat gaaactgata agaaaacttt ggaagaaact tacaaaaaag
     2701 aaattgaaga aaaagatttt aattactggt taggtgatgc ttttaaatct gttaaatgta
     2761 gagatgaaat agcattaaaa gctcatttag atatagaagc actaagaaat agtaaagaca
     2821 gacaaaaagc ttttgaagag caaataaatc ctttgaaaca ggataaagat tatttgttta
     2881 atgcaacact agaaggtgaa ga
//
LOCUS       DAMJWU010000206         2864 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMJWU010000206 DAMJWU010000000
VERSION     DAMJWU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.212"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            49..1158
                     /locus_tag="PC460_003679"
     CDS             49..1158
                     /locus_tag="PC460_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2602522.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1535..2719)
                     /locus_tag="PC460_003680"
     CDS             complement(1535..2719)
                     /locus_tag="PC460_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602523.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1090 a          439 c          402 g          933 t
ORIGIN      
        1 ttaattggtt aataaaaaag aaaatttttc atgaaaggta agataacaat ggatttcaaa
       61 agcttaggga ttagtgaaaa tacaataaat attctaaaaa aatcaggaat aacaacacct
      121 acacctatac aaaaagaaag tattaaacta ataaaagaag gaaaagatgt aatagcagaa
      181 gctcaaactg gaacaggaaa aacactagct tttttacttc cgatatttga aaatatatca
      241 ttagatataa atgatataca agtacttata ctttctccta caagagaact agctattcaa
      301 ataacagaag aagctatgaa acttaaagaa tctaaagatg taagtattct tgcagcatat
      361 ggaggaaaag atattggttc tcaaataaag aaactaaaag gaaatattca tatgattata
      421 gctacaccag gtaggctttt agaccatcta aatagaaaaa caatagacct tagtaagtta
      481 aagacttttg ttttagatga agcagaccaa atgttgctta tgggatttaa aaatgaagtg
      541 gaagctattt taaaagagac ttcaaacaaa aagcaaacac tatgcttttc tgcaactata
      601 aattctcaag taaaaaaatt agcttataga tacacaaaaa atcctgttgt agtttctatt
      661 caaaaagaag aaatcactct taataatata aaacaagaag tagtagaaac tacagataga
      721 aaaaaattag atgccttatg taaggtacta gatgaagata atccttttat ggcaataata
      781 ttttgtagaa ctaagagaag ggttgacaat cttgaagaag ctcttgctat aagaggatat
      841 aattgtcaaa aacttcatag tgatattgct caatcaaaac gagaaagaat aatgaaatca
      901 tttagaaatt tagatataca atacttaata gctacagatg tagcttctcg tggtcttgat
      961 atatctggag taagtcatat atataattac gatttaccag aaacaccaga agactacatc
     1021 caccgtattg gtagaactgg tagagctggt gaagaaggct atacatgtgc ttttattgac
     1081 cctaaaaatg aacgaatgtt aagtgaaata gagacggcta tagaatctaa aattagtaga
     1141 agaattatag aattataatt tatagattgc ttaaaatacc aatgccacac aaacactgta
     1201 atatatagaa agctacagaa aacatgtatc aatctatact ttgctgttat actattttat
     1261 attaaagaaa aaagaggcta tccaaagtag gattagtctc ttttattgtc aaaataatga
     1321 aaatatattt gacttcatat aaaaaagagt gccttataaa ctaaaagtcc attttgagac
     1381 accctctttt tatcttttat tcttagtttt atattgcttg tttatagttg tttaatatta
     1441 gattttgcaa ataatcttaa taaacatgca gatatattat ctatagtatt cttatctata
     1501 gtattctctt tagcaatatt ttttgctact acatctaatt tacattttca tttaattccg
     1561 ctggccattt agcttctgga ttttcctttg caaacttctt attcttataa tgtcctagtg
     1621 taaggaatgg aattactata gtgaatagac caagataacc acaatatcca tatccatatt
     1681 taacaatatt acttaatcct gtaagtgata taagcattga cactaccatt attgcacaag
     1741 ctgtaaatac ccttcttttc attcttgaag aaaggaaaga aagtgcttta tggtcttcaa
     1801 atctatttac aagaccaaat atagtagtta ctcctgtaga cattaaacaa aggaataatg
     1861 caacattata aattatgtat atagtatgat ttccagattg acctgcaaca taaagagtag
     1921 ggagtgttgt ttgcccagca gcaataattt ctctatacca tcccattagc atagttacag
     1981 caagtcctaa tgcaattgca ttcataacaa atgaaattat cattgcctta gtagcttctt
     2041 ttgagttctt aagaagtggt cttgaacatc ctgcaagtgt aggtataaca accgattgaa
     2101 aacctgcata aataaaagta ttaagtattg gcttgatata gccaggtgtg aaactcgttc
     2161 tatttgataa tatttcagtt atttctggta ctttagcttt tataccaaca ataaaaatag
     2221 taagagtaca tactagaata gctattgtca ttgctgtagc tgctttagat acaagcatag
     2281 caccaaatat agtaaagaca agaagtacaa ctccaataat aaatacagct acaaaataat
     2341 ttactcctat actttgaaat actgctgctg cacctgctat aacagcactt acagctatta
     2401 aaaccattag atagtaatat atctcccata ttatttcaag ttttggatat ggtctccaaa
     2461 gctcacaaaa taaatcctta taattcctac aattgttatt attatacatt atgataactt
     2521 ctctcatggt agcagtaagt ataatcattg ctaaaattgc cataagtggt gctgtccatc
     2581 catactgcac atagtactga gtttcttgat tccctgtagc aaatcctcct cctgcatgtg
     2641 aactaaaaag aacgcttgca acaccaaaaa tggctaacat agagcccgtt acttttgctt
     2701 tctttacact tttttgcatg aaatcatcct ccctaaaatg atattaaaag tttttatttt
     2761 aacactttat tgataaaaca ataacatttt tgctcattaa attcaactat atttttccaa
     2821 tgactttaca ttttcaactt atttctacct ttatattaaa tatt
//
LOCUS       DAMJWU010000207         2829 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMJWU010000207 DAMJWU010000000
VERSION     DAMJWU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.213"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            104..2239
                     /gene="rnr"
                     /locus_tag="PC460_003681"
     CDS             104..2239
                     /gene="rnr"
                     /locus_tag="PC460_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF2602524.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1243 a          264 c          531 g          791 t
ORIGIN      
        1 gaaaatatag aaaattatgt gataagtaaa aaatgttttg tctgaagaaa tataaaagac
       61 aatataaata ataatataat gaaaagtaaa acaggaggga aaaatgatac caggacttaa
      121 agaaagtttg ctaggattaa taaatgaaag tgcatataat cctctaaaaa aagaagaatt
      181 agcagaaata tttgatatac attcatcaga aatgccaatg ttttataatt ttttaaatga
      241 attagaagaa gatggttata tctgctttac taaaaaagga aaaatagtat ctcccaatca
      301 aatgggatac tttgttggaa aatttgtatc acacaaaaag ggttttggat ttgtagaatc
      361 tgatgttgaa tatacacagg atttatttat ctcaagtgac aatataaatg gtgctatgca
      421 taatgataga gttatggcag aaatagtagt acctgcaact gaagaaaaga gagcagaagg
      481 aagaatagta aaagtaataa agagagaaat aactagaata gtaggtacct tccaatcaag
      541 taaaactttt ggatttgtaa caccagacaa caagaaattt actaaagata tatatatacc
      601 taaaaaacat tttagtggag cagtagataa tgataaggta gtatgtgaaa ttacagtatg
      661 gccacaagag gatagaaaac cagaaggcaa aattatagag atactaggtc aaaaaggtga
      721 aagaggcgta gaaatagatt ctataataag agaacatggt cttccagaag aatttccaaa
      781 gaaagtattg caagaagcag aagctgtagc tgttgagatt cctcaagaag agataaaaag
      841 aagaagagat ttaagagatt taaacatatt tactatagat ggagatgacg caaaagactt
      901 agatgatgcc atatcaatag aagtattacc taatggaaac ttcaaactag gagttcacat
      961 agctgatgtt acacattatg taagagagaa aaataaactt gacaaggaag ccttaaaaag
     1021 agctacatca gtttatttag tagataaagt aattccaatg ttgccaaaaa ctctttcaaa
     1081 tggagtatgt agcttaaatc catttgaaga taagctaact ctttctatat ttatggaaat
     1141 agaccataaa ggaaatgtgg ttaaacatga aatatgtgaa tctgtaataa attcaaaagc
     1201 tagaatgact tatacagaag tatctgatat attagaaaag gatgatgaaa aacttaaaaa
     1261 gacttttgaa catgttgttg aagattttaa aaatgctgaa attttagcta ggattcttat
     1321 gtcaagaagg gaaaaaagag gagctataga cttcaatttc ccagaggcaa agataattct
     1381 aaatggaaaa ggcgaagttg tagatataaa gccatatgaa agaagaatat ctaataagat
     1441 aatagaagaa tttatgctta taagtaatga gacaattgcc gaacattttt actggatggg
     1501 aataccgttt gtgtatagaa tacatgagac accatctttg gaaaaaatgg aagaattgag
     1561 taagtttata tctacatttg gatatataat aaaaggtgat aaagaggaag tacatccaaa
     1621 agcattacaa ggtataatag agaaaataaa gggcaaaaaa gaagaaggag ctatcagtac
     1681 tataatgctt cgttctttaa aacaagctaa gtactcacca gaatgtagtg gacattttgg
     1741 attagcagct aaattctact gtcactttac ttcaccaata agaagatatc ctgaccttca
     1801 aattcataga ataataaaag aatctttaaa taataaaata agtggaaaaa gacaagaaca
     1861 gcttactact atagtggatt atgcatctgt tcaatcatcc gagaaagaaa gaaaggcaga
     1921 gttagctgaa agagatgtac atgatttcta taaggctctt tatatgaagg ataaagttgg
     1981 tcaagaattt gagggtgttg tatctagcgt aacttcattt ggaatatttg ttgagttaga
     2041 taataccata gaagggttga taagacttgc aaatatgaat gatgattact atatttatga
     2101 tgagagttct tatagtgttt taggagaaag aactaagaag tcatataaga taggtgacct
     2161 tgttaagata aaggttgaaa gtgttaatgt agattttaaa gaaattgact ttgagatagt
     2221 tgaaaaaata gaggagtaga taaaacagct aaaataagag ataacaagtt gtagtattta
     2281 tgatatgtat aaatatatag tgcacaatac tctataaaat aaatgataaa aataggtaat
     2341 tgagaaatat ctaatggaaa tagttaactg aaagataaat aattaagaaa tatttaatga
     2401 aagtagctaa ttgaaaaata agtaattgag aaatgtttaa tagaaagagc taactgaaag
     2461 ataagtaatt aagaaacatt taatagaaaa agccaactga aaaataaata attaagaaat
     2521 gtttaataga agtggctaat tgaaaaataa gtaattaaga aatgcttaat agaagtggct
     2581 aattgaaaaa taagtaatta agaaatgctt aatagaagtg gctaattgaa aaataagtaa
     2641 ttgagaaatg cttaatagaa gtggctaatt gaaaaataag taattaagaa atgcttaata
     2701 gaagtggcta attgaaaaat aaataattaa gaaatgttta atagaagtga ctaattgaaa
     2761 aataagtaat taagaaatgc ttaatagaag tggctaattg aaaaataagt aattgagaaa
     2821 catttaata
//
LOCUS       DAMJWU010000208         2791 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMJWU010000208 DAMJWU010000000
VERSION     DAMJWU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.214"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            375..2186
                     /locus_tag="PC460_003682"
     CDS             375..2186
                     /locus_tag="PC460_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2602525.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2274..>2791)
                     /locus_tag="PC460_003683"
     CDS             complement(2274..>2791)
                     /locus_tag="PC460_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602526.1"
                     /translation="QSIYKFIAKIPFVKKDNSNFNYTNYYMEFEDIPEYYEVNFNNYL
                     YIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFINGETLVDSYNFPNNEVKDYIQK
                     RESLYIETKKYYEEKGLKIERHALGSEDGEYVELTNDSILYLLDIEAVEIREQSKNIE
                     EFLYKYKKRFK"
BASE COUNT         1053 a          287 c          403 g         1048 t
ORIGIN      
        1 ttaaattgta ggtaaatagt attttaatta tttattaaaa tatagattat gtatatgcca
       61 aatttaaaaa gagacataaa gacattatac aaatatatta ttggggtgat attttataag
      121 aaaagaagtg atgcagtaaa ttaattgagc aatttaaaaa tgatttaatg atagtgttga
      181 tgaatgttta aaaagaaatt ttagaaaaac gtatattgct gtttgtgttg gaggtgatga
      241 atttgtataa tttataggat ataaatgatt aaactattta agataaatac atatcactgt
      301 aaaatattga taatcatttt ttataagaga ttgattattt gcaaattata tatattacaa
      361 atgaggtaac aataatgaag aaaaaatatg attttttaat ttattctgct atttggctag
      421 ttattattat tctttttata ttttctctat atcacagtat agagcatatt aaaattataa
      481 gtcacactgg tactattcgc agtgaaactc aaaaggttgt taagcaagaa ttgaataatg
      541 aaagaaatga tgacttgata aaacgactgg ataatattct gataaagcta agaacaggta
      601 atggagaaaa tggatttcaa agatgtgata ataaagaatt tcaacaaaaa ctaaatcaaa
      661 tggattctat gtgggaatct atgaagaaag aaattataaa agttagaaat ggtgcttcag
      721 gtgataaatt atataaattg agtgaagagt attctgtgtt gtctaatcaa attgttttta
      781 tatctgaaaa acactctaat gcaaaattgt attcttttgc tactgcacta tttatttatc
      841 ttatattctc aacaataagt cttttaattt gggaatatta taataaaaaa aggtttaaaa
      901 gaatatttta tactgataat ttaacaaaaa taaaaaatca ggttgcattt gaaaacagag
      961 cgattgaaat tttgcacaat gcatctaaca aagagtatgt actgctaaat attgatattg
     1021 acaattttaa atatattaat gatacacatg gatatgaata tggagataaa gttctaataa
     1081 tagttgcagc tgcactttct aaaactttta atatcaaaga aacttgtgca agaattggtt
     1141 cagataattt tgtaatactt gcaaaatata gagattctct tttagaggat attcgggaaa
     1201 tgctaactaa tgctataatc tctgaattag atatgaatgt aactcaaact atatcttatt
     1261 gtataggagc gtacttagtt gaaattgata atttaggtta taaatccatc aattctataa
     1321 tggataaggc aaatattgca cacaaggtaa gtaaaacaag aggaatttcc tccacagtat
     1381 ggtataatga aaatctgtta aaacagttac aaatggaaaa tagtatttat aattatatgt
     1441 ataaagcgat agaaaatgaa gaatttcata tgtatttgca acctaaattt caaatatctt
     1501 ctttaaatgt tgtaagcgca gaagctcttg taagatggtt ttctccagaa cttggttttt
     1561 tatcaccaga tgaatttatt ccattgtttg aaaaaagtgg atttattatt gaattggact
     1621 tttatatgtt aaaaaaagcg tgttcatttg taagaaaaac gtttatgaaa aaaaatcaat
     1681 atacatatcc gattgcagtt aatttttcaa gggttactat ttaccaaaat agtttttatc
     1741 aaaggttttt agatactgtt aaagaatatg aaattccttt taagtatata gagatagagg
     1801 taactgaaag tgcttttaat gaaatatctc aaccagttat ttcaatttta gaggaactta
     1861 aaaagttagg atttttaatt tcaatggatg attttggttc aggatactct tcattaagtc
     1921 ttttatgttc tctatctatt aatggattga agttagataa aagcttactt aaagaaacct
     1981 ttaaccggga gaaagtatat agtattattc aatgtattat tgaaatgtca catcgtatag
     2041 gcatgtctgt tgtatgtgaa ggtattgaaa ctaaaaaaga cttggaattt ttaaatacag
     2101 ttaaatgtga tgtaggacaa gggttttatt tttctaaacc gatagaagaa aaagaatttt
     2161 ttaacaaata tgtaactaaa aaataatata attattttta tcagtctata cattgagtca
     2221 ttttatatct cgttaaatga ctcaatgtac tgttatgtat tttaaaatat aaattattta
     2281 aaacgttttt tatacttata tagaaattct tctatatttt ttgattgttc tcttatttca
     2341 actgcttcta tatctagtaa ataaagtata gaatcatttg taagttcaac atattcacca
     2401 tcttctgaac cgagtgcatg tctctcaatt tttaatcctt tttcttcata atattttttt
     2461 gtttctatat ataatgattc tcgcttttgt atgtagtctt tcacttcatt atttggaaag
     2521 ttatatgaat ccactaatgt ttcgccattt ataaaagttt tctcggtagc aactttttca
     2581 tcccaacaat ggatttcttc ccaagtaaaa aatatctcag ttccatctgg aatgaatgaa
     2641 aacctggtaa taatatataa ataattattg aaatttactt cataatattc aggaatatct
     2701 tcaaattcca tatagtaatt tgtataatta aaattactat tatctttttt tacaaagggt
     2761 atttttgcaa taaatttata tattgattgt t
//
LOCUS       DAMJWU010000209         2744 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMJWU010000209 DAMJWU010000000
VERSION     DAMJWU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.215"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            133..1056
                     /locus_tag="PC460_003684"
     CDS             133..1056
                     /locus_tag="PC460_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF2602527.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1031..1858
                     /gene="panB"
                     /locus_tag="PC460_003685"
     CDS             1031..1858
                     /gene="panB"
                     /locus_tag="PC460_003685"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF2602528.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            1877..2725
                     /locus_tag="PC460_003686"
     CDS             1877..2725
                     /locus_tag="PC460_003686"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF2602529.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1094 a          329 c          450 g          871 t
ORIGIN      
        1 tatcttgtta taagaactat actttaaatg tttttaatta ttttgtctta tgtaactcat
       61 atcctcagga atgagattag aaattaagat gttgtatcac aaagtggagg tgatactatt
      121 aaaataggct ttattggttc tggaaaagtt ggaacatctt tggcaaatta ttttttatca
      181 aaaggttatt gtgtttcagg cttttatgga aggaatcaat tatctttatt agaatcaaca
      241 aatttaacaa aaacaaagat ttacagcaat ttgaaagata taatttatga aaacgatata
      301 ttatttatta caacatcaga tgattctatt gaaattatag acaaaaaact ttccaaattt
      361 aatttaaaac ataaatacat ttgccacaca agtggctctt taaaatctag tatattgttc
      421 tgttcaaaaa aagcaggtgc attaatttat tctatccatc ctatatttgc attttccaat
      481 aaaaatacca acataaaaaa aatgaaagac atttgttttt ccatagaagg tgataatgaa
      541 caagatgatt tagttattca aaatttcata gatgatttgg gaaaccaatt ttttataaga
      601 gataaggata cttcatccac ttatcattta gctaatgttt tagtttcaaa tttagtatta
      661 tctttactag atataggagt aagttacttt attaaattag gtttatctga agaggaatct
      721 ttaaaagcta tcagtccttt agtaaaaaaa aatatagaaa atatacttga taatggcttt
      781 actaaagctc ttactggtcc tgtttctcgt ggagatataa caccaataag aaaacatcta
      841 tctgtattgg ataaaaaaga tgaggatatt tacaaaatac tatccttaaa cctactaaaa
      901 ataattgcct taggaaatga caattcatta aaaggtataa aaaatattac tatagaaaat
      961 gcaatcgaaa atttaataag taaatctgaa aaatacaaag aaatttacaa acttttagga
     1021 ggaaaaaacg atgaaaaata ccatacagac ttttaagaat gctaaatatg aaggaaaaaa
     1081 actatctatg cttactgctt atgactactc aattgcaaaa ataatggatg agtgtgatat
     1141 aaatggtatt ttaataggag attcattagg aatggttata aaaggtgaag aaaacactct
     1201 atctgttacc atagatgaaa ttatatatca tacaaaagct gtaaaaaatg gtgtaaaaaa
     1261 tgccttaatc gtaagtgata tgccttttct atcttaccat gtttctatag aagatgcagt
     1321 taaaaacgcg ggtagactta taaaagaagg aggagctcat gctgtaaagt tagaaggtgg
     1381 ctcaaacgtt ataaagcaaa tagaaagcat tgtaaatgct caaatacctg taatggggca
     1441 tttaggatta actcctcaat cagtaaattc atttggtggg tttaaggttc aaggaaatac
     1501 cagtgaaaca gcaagacaac ttattgaaga tgctaaatta atagaaaaag caggtgcatt
     1561 ctctattgtc cttgaaggag tgcctacgaa aatagctgag atggtaacta attctatttc
     1621 aattcctaca ataggtatag gggcaggaat taactgtgat ggacaaattt tagtttatca
     1681 agatatgttg ggaatgtttg gagattttgt tcctaagttt gtaaagcagt atgcaaatat
     1741 aggagatata atgaaagatt caattaaaaa ttacatccta gaagttaata caggtacatt
     1801 tccacaagaa aaacacagct tttcaataaa tgaatctgag ttagaaaaat tatactaata
     1861 ttaaggatgg tacaaaatgc ttgtaaaaga aattaaatta ctaagaaata taatcaaaga
     1921 ttggagaaaa catggctatt ctatagggct tgttacaaca atgggatttt tacatgaagg
     1981 acaccaaagt cttataaaaa aagctgtaaa agaaaatgat aaagtagtag tgagtgtatt
     2041 tgtaaatcca actcaatttg gaccaaatga agattttaat agctatccaa gagatataga
     2101 taaagatttt aaatattgta tggatagtgg agcaacagta gtctttaacc catcaccaga
     2161 agaaatgtat ttaaaaggta attgtactac cattaatgtt tctggactta cagattttct
     2221 ttgtggagct aaaagacctg ttcattttgg aggtgtctgc ctagttgttt caaagtttct
     2281 aaatatagta acacctgata aagcttattt tggagagaag gatgcccaac aattagctgt
     2341 aattaaaaga atggtcaagg atttaaacat agatacagaa ataataggct gtcctataat
     2401 tcgtgaaaat gatggattag caaaaagttc gagaaataca tatctctctg aggaagaacg
     2461 aaaatctgct ctaatattaa ataagagctt aagtttagca aaagaagaat tagtaaaagg
     2521 caatctaaat ccagagaata taaaagaatt aataacagct aaaataaatt ctgaacactt
     2581 agcaaaaata gactatgttg aaattgtaga tagtgagaca ttacaacctg ttaagcaaat
     2641 agaacattct atacttgtgg caattgctgt gtttatagga aaaaccagac taattgataa
     2701 ttttacattt gagttaaata tatagtctat aattttatat aata
//
LOCUS       DAMJWU010000210         2622 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMJWU010000210 DAMJWU010000000
VERSION     DAMJWU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.216"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(226..1476)
                     /locus_tag="PC460_003687"
     CDS             complement(226..1476)
                     /locus_tag="PC460_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF2602530.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1509..2450)
                     /locus_tag="PC460_003688"
     CDS             complement(1509..2450)
                     /locus_tag="PC460_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF2602531.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          791 a          499 c          244 g         1088 t
ORIGIN      
        1 aatacacatt taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat
       61 tatagcatag aatatgacta tctatatcct aaaattatcc atttctaaaa aaaaggtaac
      121 tattattaag aatatcaact tatagaattt atattccaca tacgattttc aatcaaaaaa
      181 acaagggcta aaaagtgaat tgtgcacttt ttagcccttt attaattatt catttatttc
      241 atgtaagaat agaccacatc ttactttagg ttcaaaccat gttgacttag ctggcattat
      301 cttatttgca tctgctactc ttattaaatc ttcaatacta gtaggataca ttgcaaatgc
      361 tactttcata tcttcattaa ctcttttttc taattcttca agacctctaa ttccaccgat
      421 aaaatcaatt cttttatcta ctctaggatt atcaattcct aaaatagcat caataacata
      481 gtcttgtaat atagaaatgt ctaatgagtc aacagggtct tctatttcta ataaattttt
      541 atttgctttc attttatacc acttatcacc taggtacatt ccgaaagttc cttttttgtc
      601 aggtttatat ttttcttttc cttctatttc ttctagttca aaattttctt ttattttatt
      661 tataaattct tcctcgctta aaccatttaa gtctttgata accctattat aatccaaaac
      721 cattaaattc tcatctggtg caatcattgc tatgaagtaa ttaaattctt cttttccagt
      781 ataatttgga tttttagctc tcatttcttt ggctacattt tctgctgaag ctgtcctatg
      841 atgaccatct gcaatgtata gataatctaa tttttcaaat ttatctacta ggttatctat
      901 tatatcatca ttatcaatct tccatactgt atgctctatc ttgtcatctg ttatgaaatc
      961 atataaaggt tcattattat ccataaaatc attgattata tcatctatca ccctttgatg
     1021 tttgtaagtt acaagtatag ttcccgtatt agcatcacag tattttatgt gtttagttct
     1081 atcaatttcc ttttctggtc gagtgtactc atgttttttt attgttccat tcaagctttc
     1141 atcaactgat atacatgcta ctataccaac ttgagctcta ccatccatga tttgtttata
     1201 tatatatata cactctttgt catcttttac tagaatttct tgttttcgaa actcatctaa
     1261 attttctcgc gcttttaaat aaactttttc atcatataaa tctatatttt catctaaatc
     1321 aatttctgat ttatctatat gcaaaaatga atatggattc ccttctgcaa ttcttctagc
     1381 ttctttacta ttcataacat cgtaaggaag tgctgctact ttatctacta tatatttatt
     1441 tggtctaatc gccttaaatg gtttaatcgt tgccattact aatttcctcc ctaattatac
     1501 aaaatatttt ataatgctac ccctattaga ttgttgaagt caagaaagaa atcttgaatc
     1561 acatttacta tttcgtctcc tattctttct tgagcttcta ctgtagatgc cccaatatga
     1621 ggtgtaggag aaactcttgg atgatttact aagtctaaat ttaatgtagg ttcttgttca
     1681 taaacatcta tagcagctgc tgataattta ccttcatcta atgcttttag taaatctttt
     1741 tcatcaacta aaccgcctct tgcacagttt attaaataag cccctttctt catcttattt
     1801 atttcttttt cagttattac tgccccctta ttcttatcaa atggtatgtg taaagataag
     1861 aaatctgcat tttctaaaac ttcttccata tcacagaatt tataattgtt aaagccttgc
     1921 gcctcaccca ttatgtcagt atatataaca ttcataccta ataattctgc tctctttgca
     1981 acttcttttg caattcttcc aaaacctata agtccaagtg tctttccatt aatttctgta
     2041 cctttatact ttttcttctc ccattttcca tctctcatag ttacatttgc agtatttatg
     2101 aatcttgcaa gaacaaataa ttgacctatt gtaagctctg caacagatat actgcttgca
     2161 tttggagtat tcataacttt tatgccatta gcttgagcat atttaacatc tatattatcc
     2221 agacctactc cacctcttac tatcaacttc aactttttac cctctaatgc tttatctatt
     2281 acatctttag taactttagt agctgaacgt actacaagta catcaacatc tttaagttta
     2341 tctcctaaaa catctttttc ataaaactgc tctatcacat taaaatctaa ctctctcaat
     2401 tttctcgctg cttctttctc tattccatca gtaactaata tattatacat aacaaactcc
     2461 tccaattttt attcacttaa tttttccaat ttttattttt taaagtattt tcatcaataa
     2521 aaaactttac ttaatttaac tttctgttta tataattttc aaaatatttt tttattaaag
     2581 tcatatcaaa ttttcctatt gtttgttaaa aatttaatat gt
//
LOCUS       DAMJWU010000211         2569 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMJWU010000211 DAMJWU010000000
VERSION     DAMJWU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.217"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..438
                     /locus_tag="PC460_003689"
     CDS             <1..438
                     /locus_tag="PC460_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2602532.1"
                     /translation="EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKF
                     KKAIRELLWFVCEYLKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDET
                     IVSNHPWVEDVNDELERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            428..1825
                     /locus_tag="PC460_003690"
     CDS             428..1825
                     /locus_tag="PC460_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2602533.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAMNSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIRHGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRHIYTSGYYHAAFNVQQGVNVGWS
                     LMNLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKNLV
                     NDFSKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLKTSNICRE
                     LDGKVFDMKDYQVGVTAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSSMKYN
                     EWYEKYVKHNSILEIKNSAIIDSIKEDIKNGKYNLNIHDGKQGKHLKEHNNYIEGRSY
                     LTITKEEAQELVNKHAGNGIIKFNRSGEWDKKELIEVDKNIGVNVNNITGEKTLTNKF
                     KIHYSKTGTHIVPAL"
     gene            1840..2040
                     /locus_tag="PC460_003691"
     CDS             1840..2040
                     /locus_tag="PC460_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602534.1"
                     /translation="MKLWEYVGKNVQITCVDKQIIRGKCDGYTQALDNEPEIASISIA
                     RDGYGIEVYENEIESIENINKE"
     gene            2106..>2569
                     /locus_tag="PC460_003692"
     CDS             2106..>2569
                     /locus_tag="PC460_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2602535.1"
                     /translation="MDWLKELLEGIKIEDNKIDVASLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGE"
BASE COUNT         1111 a          256 c          490 g          712 t
ORIGIN      
        1 gaaaagaata taattatctt tggtcaagga gttaatccag aatctcaaaa cacaggtgac
       61 aaatcgggtg tagcacttaa atttttatat tcattactgg accttaaatg ttctaagact
      121 gaaaagaagt ttaaaaaagc aattagagag cttttatggt ttgtgtgtga gtatttaaag
      181 ataagtggta gtaagagcta tgattataaa acagttcaaa ttacttttaa tcactctatg
      241 ataataaatg aagctgaaaa gatagatatg gcagctaaat caactggaat tgtatcagat
      301 gaaactattg tttctaacca tccttgggtc gaggatgtaa atgacgaact tgagagactt
      361 aaaaaacagg aagatactca aaaagagtat gatgatttaa ttcctaataa tcaagatggt
      421 gttatagatg aaacataaag attattggag gaagagattt gaacaattag aagaagctca
      481 aaataacaaa agtgtaaaat attatcttga attagaaaag caatataaac tagctatgaa
      541 tagtatagaa aaagatatat tagcatggta caacagattt gccaaaaatg aaggaatatc
      601 tttattagaa gctaagaaac tactaaatac aagagaacta gaagagttta aatggagtgt
      661 cgaagaatat attagacatg gtaaagaaaa tgctataaat caaaagtgga tgaaagagtt
      721 agaaaatgct agtgcaagag ttcatataac aagacttgaa gctttaaagt tacaaataca
      781 gcaacaagtg gaagtgcttt atggaaatga acttgatggt attgataaat taatgagaca
      841 tatttataca agtggatatt atcatgcagc ttttaatgtt caacaaggag taaacgttgg
      901 ttggagttta atgaatcttg atactaatag aataaataaa gttatctcta aaccatggac
      961 tagtgatgga ttaaatttta gtgaaaggat ttggggcaaa catagacctg ctttaattaa
     1021 tgagttacat actaagctaa ctcaatcaat tattagaggt gaaaatccaa agaacctagt
     1081 aaatgacttt tctaagagat ttaatgtatc taaatcacaa gctaagaatt tgataatgac
     1141 tgaatcagct ttctttgcat cagcttcaag aaaagattgt ttcaatgatt tagatgtaga
     1201 gaagtatgag attattgcca cactagactt aaaaacatca aatatatgta gagagttaga
     1261 tggaaaagta tttgatatga aggattatca agttggagtt acagctccac catttcattg
     1321 tcgttgtagg acaacaacag ctccttggtt tgaggatgaa gaaggttaca gagcagcaaa
     1381 aggagaagat ggaaaaacat attatgtacc atctagtatg aagtataatg agtggtatga
     1441 gaagtatgtt aaacataata gtatcttaga aataaaaaat agtgctataa tagatagcat
     1501 aaaagaagat attaaaaatg gtaaatataa tttaaatatt catgatggga aacaaggaaa
     1561 acatttaaaa gagcataata attatataga aggaagaagc tatttaacta taacaaaaga
     1621 agaagctcaa gaacttgtga ataagcatgc tggcaatgga atcataaaat ttaatcgaag
     1681 tggagaatgg gataaaaaag aacttataga agttgataag aatatagggg tcaatgttaa
     1741 taatattacg ggtgaaaaaa ctcttacaaa taagtttaag atacattatt ccaaaactgg
     1801 aacacatata gtaccagctt tataaggagg aaaaataaaa tgaaattatg ggaatatgta
     1861 gggaaaaatg ttcaaataac ttgtgtagat aagcaaataa taagaggaaa gtgtgatgga
     1921 tatacacaag ctttagataa tgaaccagaa atagcaagta tatcaatagc tagggatggt
     1981 tatggaattg aagtttatga aaatgaaata gaatctatcg aaaatataaa taaggaatga
     2041 ctaagcatgt acttaaaaat aagtagatgc ttttattttg taaaaaatga aaggagaaat
     2101 ttaaaatgga ttggttaaaa gaattgctag aaggaataaa aatagaggat aacaagattg
     2161 atgtagcttc tttacaaaag tctatagaaa agaaaataaa agagactaca attactcaag
     2221 aagattatac aaatcttgaa acacagctta atacagctaa tgaaactatt aaaaagtttg
     2281 aaggaggtat gacaaaagaa gatgtagaga atctaaaaac aacttatgaa actgataaga
     2341 aaactttgga agaaacctac aaaaaagaaa ttgaagaaaa ggactttaat tactggttaa
     2401 atgatgcttt taagtctatt aaatgtaggg atgaaatagc gttaaaagct catttagata
     2461 tagaagcact aagaaatagt aaagatagac aaaaagcttt tgaagagcaa ataaaccctt
     2521 tgaaacagga taaagattat ttgtttaatg caacactaga aggtgaaga
//
LOCUS       DAMJWU010000212         2560 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMJWU010000212 DAMJWU010000000
VERSION     DAMJWU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.218"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            636..2432
                     /locus_tag="PC460_003693"
     CDS             636..2432
                     /locus_tag="PC460_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDF2602536.1"
                     /translation="MKPTMEILTKLQENSKKNHDEVFTRLYRYLLRPDIYYVAYQHLY
                     SNKGAGTKGVTDDTADGFSEIYIENIIEALKNEMYQPKPVRRTYIKKSNGKMRPLGLP
                     VFTDKLIQEAIRMILEAIYEPIFSDYSHGFRPARSCHTALAQIKKEFTGARWFIEGDI
                     KGCFDNINHAVLVEIINQKIKDARFLKLIRSFLKAGYMEDWKYHKTYSGCPQGGIISP
                     ILANIYLNELDRHVMKIKKEFDVATKARYTPEYTKLVGLRQRLHNKIKNSNGIEREKL
                     IEEYKSATAQMLKLPAKQCDDKKIKYVRYADDFLIAVNGNRQDCEKIKQELTEFISTT
                     LKMELSQEKTLITHSNTPARFLGYDVRVRRDQQIKPKGKFKTRSMNNKVELSIPFKDK
                     IEKFLFSNGIVKQRSDNGKLEPIHRPQLLNRTDLEIVTIYNAELRGICNYYGLASNFN
                     KLIYFNYLMEYSCLKTLAGKHRSKVSKIRAMYKDGTGKWAIPYETKTVIKKMYFANYA
                     DCKGKKFTDIVPQTAKNYSHDVTTLESRLKAKICEVCGCTESDRYEIHHVNKVKNLKG
                     KSEWEKIMIAKRRKTIVVCHKCHMAIHHGEKK"
BASE COUNT          977 a          439 c          533 g          611 t
ORIGIN      
        1 ttagaagtag attaaagaaa gggggacaga aaaaaaaggc actaccccgt aagataacac
       61 gggaaaccac ctgcctaacc gaaaggtgaa aactgtacgg gaaaatagca cggcaggaaa
      121 gcagaaagtt gttcaaagac aatagagcac gactgaactg caaggttaag tgaatatgag
      181 gataaaacta gatttgttga atgtgagttc caaacctctg ttacgtgtgg acacaggaaa
      241 cttgtctgaa acctgtggta tgagtaacgc tgaaaataaa tgcctttcag tgggttatca
      301 attaactcat acggtacgct ggagaaccag tagtaacgga acgaaagcat atccgacaat
      361 tcacaccagc ctactacttt cgtcaactgg agattaccta aaccagaatg cctacaatgg
      421 ctatgtgtaa tgctcttgta gtgataaatc acaaggctat gctaagtgag atgacgctga
      481 aaatctggca aggtaacgga gctttcatag tagtctgaga accctgaaaa gacagggaaa
      541 gccgtaaaat cggatacggg aaaaccgttc acatggcgaa ggaaagcagt cttagcgaat
      601 taaaattaga aattgattag ggaggaaaac ctcaaatgaa accaacaatg gagattttaa
      661 ccaaattaca ggaaaattca aaaaagaacc atgatgaagt atttacaagg ctctatcgat
      721 atttactccg tccagatata tactatgttg catatcaaca cctctactcc aacaaaggag
      781 caggaacaaa gggtgtaaca gacgacacag cggacggttt tagcgaaata tatattgaaa
      841 atatcattga agccttaaaa aatgaaatgt atcaaccaaa accagtaaga cgtacataca
      901 tcaaaaaatc aaatggcaaa atgcgtccct taggactgcc tgtctttact gataagctga
      961 tacaggaagc aatacgcatg atattagaag caatctatga gcctattttt tcagactact
     1021 ctcacggttt ccgaccagca agaagttgtc atactgctct tgctcaaata aaaaaagaat
     1081 ttacaggagc acgctggttt atagagggcg acataaaagg gtgctttgat aacatcaatc
     1141 atgcggtact tgtggaaatt atcaatcaga aaattaaaga tgcacgtttt ctgaaactca
     1201 ttcgttcatt cttaaaggca ggttatatgg aagattggaa ataccacaaa acatatagcg
     1261 gttgccctca aggtggcatc atttcgccta ttcttgcgaa tatctacctt aatgaattag
     1321 accgtcacgt aatgaagata aagaaagagt ttgatgtggc aacaaaggca cgttatacac
     1381 cagaatatac aaaactggta ggactacgcc aaagattaca caacaaaatt aaaaatagca
     1441 acggtataga aagagaaaag ctgattgaag aatataaatc tgcaacggct caaatgctta
     1501 aactaccagc gaagcaatgt gacgacaaga aaataaaata tgtgcgttac gctgatgatt
     1561 tcctaatagc tgtgaatggc aatcgacagg attgtgagaa gattaagcaa gaacttacag
     1621 aatttataag taccacatta aaaatggaac ttagtcaaga aaaaactctg attacacaca
     1681 gtaatactcc tgctcgtttt ctcggatatg atgtcagagt acgccgtgac cagcagatta
     1741 aaccaaaagg aaaattcaaa acccgttcta tgaataacaa ggtggaattg agtattccat
     1801 tcaaagacaa gatagagaaa tttttatttt caaatggaat tgtaaagcag aggtcagaca
     1861 atgggaaatt agaacctata caccgaccac aactgcttaa taggacagat ttggaaatag
     1921 tgactattta taatgcagaa ttaagaggta tctgtaatta ttatgggttg gcaagcaatt
     1981 ttaacaagct aatctatttc aattatctta tggaatatag ctgtttgaaa acacttgcag
     2041 gaaagcaccg ttccaaagtt tctaaaatta gagctatgta taaagacggt acaggcaaat
     2101 gggctattcc ttatgaaact aaaacagtaa taaagaaaat gtattttgcg aattatgctg
     2161 actgcaaagg aaagaagttt actgatattg ttccacaaac agctaaaaat tattcccatg
     2221 acgtaacaac attagaaagc cgattaaagg cgaagatatg tgaagtatgt gggtgtacgg
     2281 aaagtgacag atacgaaatt catcatgtaa acaaggttaa aaaccttaaa ggaaaatcag
     2341 aatgggaaaa gataatgata gccaaaagac ggaaaacaat cgtggtatgc cacaagtgtc
     2401 acatggctat acatcatggc gaaaagaaat aacctgtaac ggagcaaaga tggagagccg
     2461 tgtgcattga gaaatgcaag cacggttcgg ggagaggact gcacaaacct accctagaaa
     2521 taaggaaagg cggtgctttc ctactctact atgtgggaaa
//
LOCUS       DAMJWU010000213         2533 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMJWU010000213 DAMJWU010000000
VERSION     DAMJWU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.219"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            133..2151
                     /locus_tag="PC460_003694"
     CDS             133..2151
                     /locus_tag="PC460_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2602537.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1057 a          300 c          419 g          757 t
ORIGIN      
        1 ttttataatt tttttagaag tttatttttt atgtacttat ttataatttt gggtaaaaac
       61 actttgatat aatttcaaaa attaaaaggt taaaaaacaa agtagaaatt tatgttaaat
      121 aaggagatta atatgagaag aaatacaaaa ttattaacaa cagggattct ttcaatggca
      181 atcgtcgcac ctacaatggc atttgctact gaatctaatg ctatggaaaa taacgctgat
      241 ttaaatataa acttagagaa aaaaagtatc gttttaggta gtacctcaaa agtcagtgtt
      301 aagtttaaag aaaaaccaga tgcagatagc attacattaa agtataaatg ctatgatatg
      361 ccattggata caactttaaa ctacaatcaa tcaactgagt catatgaagg aactatcaat
      421 tataataaag acccagaata tctaaatgtt tgggaactac aagggataac aataaacagc
      481 aaaaataatc ctaaaacttt aaacaaacaa gaactagaaa agatgggatt aaatttaaaa
      541 gactataatg taacacagga atgtataatt gaagatataa cttctagaaa agatgtaaat
      601 aaatatttaa gaaaaacttc tgcacctatt acagaactta caggaagtga tagatacgaa
      661 acagcagtta aaataagtaa agaaggctgg aaaaatggtt cagataaggt agttataata
      721 aatggggatg taagtataga tggcattata tcaactccac tagcaactac atataatgcg
      781 ccaatacttt tggttgaaaa aaacaatgta cctaatagtg taaaatcaga attaaagcgt
      841 ttaaatccta gggatgtaat tataattgga gatgagaatg ctatttctaa aactactgct
      901 aatcaaatta aatcgactgt aaatgctagt caaacacgtc taaagggctc taatagatat
      961 gagacatctt tattgatagc aaaggaaata gataaaaatc atgatgtgga aaaagtgtac
     1021 ataacaaatg ctaatggcgg agaagtggat gcacttacta tagcagcaaa agcaggtcaa
     1081 gacaagcaac caattatatt aactgataaa aatagtatta cagacaacac atataaatgg
     1141 ttaaagagtg aggatttaca aaatgcttat tttataggtg gccctcaaat gatatcaaca
     1201 aatgttataa ataaggtaaa tgatataact aaagataatg ttactaataa tagagtatat
     1261 ggagcagata gacacgaaac aaatgcaaac gtaataaaaa aattctatac agatgatgag
     1321 ctagaggctg ttttagtagc taaatcagat gtacttgttg atgctttagc agcaggtcca
     1381 ttggctgcca acttaaaatc tccaatactt ataacaccaa agacgtatgt atctgcatac
     1441 cataaagaaa acttagaagc taaatcggct aataaggtat ataaaatagg aggaggattg
     1501 acttctaagg taatgaactc tatagcatca tcattatcta aacacaatac gactccaaca
     1561 gaaccaggaa atagtggggg taagacagtt atgattgacc caggacatgg tggttcggac
     1621 actggaacaa caggtaaacc attagggggt attaaagaaa aagactatac attaaatact
     1681 tcacttgcga caactgaata tttacgttca aagggattca atgtaataat gacaagagat
     1741 acagataaga ctttatctct tggaaataga actgctttat ctaactcttt gagaccagat
     1801 ttatttacga gtatacacta taatgcatct gatacaactg ggaatggtgt agaagtattt
     1861 tataagctta aagataaaga tggaggaact actaagactg tagctacaaa tatattaaat
     1921 agaatcttag agaaatttaa tctaaaaaat agaggtgcaa aaacaagaac actatcaact
     1981 gaccctacaa aagattattt atatgtttta agaaataatg atatgccagc tgtacttgta
     2041 gaatgtgcat ttttggataa tgagaaagat atgagtttac ttaatacatc aaataaagta
     2101 aaagaaatgg gtacacaaat aggtaaaggc atagaagatt cattaaaata gtctacaatg
     2161 agagcataga aaaatttaaa ataaattaat ctataatttt aaatttaaac taaagctata
     2221 agataagaat gtaattataa tagtaataaa gactgtatat acattgtgca tataaatgcg
     2281 aaatgaggta tatacagtct ttttataatt aattgtaaaa tgatttatgt tatatgaaaa
     2341 atttacatat attaaggcta cagttaattg tcaagagctg gagtattcat ttgtaataca
     2401 caatataagc ttaattacta tattttattg tgtgtttttt gtggattttt atgtcaataa
     2461 atgatatagt atagataatt aaatgtatta aatatattta atttttttat tttttgagtt
     2521 ttataagttt att
//
LOCUS       DAMJWU010000214         2532 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMJWU010000214 DAMJWU010000000
VERSION     DAMJWU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.220"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            124..408
                     /locus_tag="PC460_003695"
     CDS             124..408
                     /locus_tag="PC460_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602538.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(1179..1394)
                     /locus_tag="PC460_003696"
     CDS             complement(1179..1394)
                     /locus_tag="PC460_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602539.1"
                     /translation="MAIVVNLDVMMAKRKISLKELAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1405..1872)
                     /locus_tag="PC460_003697"
     CDS             complement(1405..1872)
                     /locus_tag="PC460_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2602540.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT         1031 a          249 c          308 g          944 t
ORIGIN      
        1 cattaaaaaa taaatttgta ttttgtttta tataaagtaa tttgaaaaat atggaaaaat
       61 tgattagtac attattaatt agggtataaa atggtgtaaa tagaatgata aggggtggat
      121 gaaatgcttg tatataataa aagtttttat cctaatgaca tatttccaag attagatttt
      181 tcaaaaataa aaaaacagtt aaaattgata gataatgacc tgtcagattt tggaagcata
      241 tgtataatag aaaaagaaca ttatacgata agtgtaaaca gtataggtga aataaatgtg
      301 tactatgatt tagagtacga aaataaggtg tatagaatag tttatgagat tgaaaagtta
      361 tttaaatctc aagttggaag gtttagcata tctacataca gaaattgata attaaaaaag
      421 tagaaattaa aaaacttaat actaaagata tagataaata tatgaggtaa cagctagaga
      481 gagttgttac cctttttaaa tactaagtat atattattct tattaatttt ttgtaaattc
      541 actaaaagac ctttttatag aatattattt tcttttttta ttaaataggt aaatcagtaa
      601 aaatgcaatt ccagttccaa ttattattcc aatgatattt tctataatta atttttttaa
      661 tataaaagta aataataaaa atgctataca taaacctaaa atatatcctt taaaataggt
      721 tgaaagtttt cttttcatat aatcaccaac ttgtatcaga tttagtttac ttaattagag
      781 tataacagtg ttgtaacttc aaagagtatc taaaagatta caaatgttag ataaaatcat
      841 caataatgta atttataaca catagctata agctatgcaa taatataaat ggaggtataa
      901 gtaaatgaaa aagttccatg ttttgctaac agtttaaata aatattatgt agaaaagaat
      961 gtatatagga atctgtggta aaattatcaa tttaaaagta ttatttagtg tgatacagtt
     1021 cttataggta atggataaca aatagataaa ggaacagttt ctttcttaga acatattttg
     1081 ttatgacttt aaacaaagtt aatattaatg tcaataatat agtaaaagaa taaaaatcat
     1141 atagtataat aaaaaagagt gtttctaaac actctttttt aatcattttc atcttctaca
     1201 tattcaagta tatcacctgg ctgacagtct aaagctttgc aaatttcgtt tagagtggta
     1261 aatctaatag cttttgcttt tccagttttt aaaattgata aatttgcatt tgtaatatct
     1321 attttttctg ccaattcttt aagtgatatt tttctttttg ccatcattac atctaggtta
     1381 actactattg ccataacatc acctctatat cgttaaatca ttatcttctt ttatttttat
     1441 agctttatca aatgtttctg caattacaaa gcacattagt gcaaatataa aagagataac
     1501 cataagtggt gagataccgc cactatcagt tccaaaaatt cttatattat tagtatttaa
     1561 attataacca ttgatacatt caattatagt gtttatcaat aaacaaaatc ccattacttt
     1621 aaaccttttt acattttcaa gtacgaatgg ggaatactta gtactattaa ttataaatat
     1681 caagaataag atgactataa agtagtcaga aaaatatagt attcttgcaa ttaaagcaat
     1741 tactttgtca gaactaaaat catataaaaa cataggtatc atacataaga atattatacc
     1801 agcaactcct aaaagtgata atagaaaata tagaaatact gataatatat tactaaaaaa
     1861 ttttgtaatc ataaaatatc atctccttta tataaagtat attattgatt ataatatata
     1921 atttattgaa tttcaataat aaaatattga tataatatat aaaaccattg taatatacac
     1981 taaaaataag aatgtataag gtggtaaatt tatataaaaa aggatatgag ttgtaaaata
     2041 ttaaataata ttgtactaat tggaataatt ttaactttat tatagattat tttaatacta
     2101 ttagtactga tggtgtattt taaaattggt ataagcatta caaatacgaa tatgccaact
     2161 gtaatataag tagctatata catatgtgct gtcccatata ttgtatcatt gtttatatta
     2221 aaaagattat gtaaattggt atcaatcaat aaatcatttt cagaagaaat acctaaaaac
     2281 ttaaaaaata cctatatgtg tatttagtta gatttgagat ttttacattt aattttatag
     2341 tgacaatatt atattatttt tatgatgtct atttatatgc aattactata atatcagaac
     2401 ttatagtatc atttgtttca ttatcaataa ggttttttac tttagttttg tcaattttat
     2461 taaaaaaagt tattgatata aaagataaga aggataaaac tatatgacgg atttcaaaca
     2521 taagagtgga ta
//
LOCUS       DAMJWU010000215         2450 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMJWU010000215 DAMJWU010000000
VERSION     DAMJWU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.221"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(176..2269)
                     /locus_tag="PC460_003698"
     CDS             complement(176..2269)
                     /locus_tag="PC460_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2602541.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT          829 a          404 c          239 g          978 t
ORIGIN      
        1 tttttattat ggttatccct ttttaaaata ctttaatcat atactattcc caatttaaca
       61 tccattgaaa tgctattttc ttatattgca atttataatt gatatatctt caatataata
      121 atttaatgta tttttgattt taaagctttt cagtgatatt attgataaat tagaattaat
      181 tatctagctt taaaaggtca aagatttctg ttgctgtctg cggttttcca aataaatatc
      241 cttgtatgta atctggcttt atttgtttaa ctaaatcata ctcttcttgt gtttctacac
      301 cttctaaaca tacttttatt cctacatcat gacaaattgc aacaataaaa tgtataaatg
      361 tagcatcaaa cttactcttt aatatatctt taacaaatac tctatcaatt ttcactatat
      421 ctattggaga aaattttaaa acttctagag acgagtatcc agtaccaaaa tcatccatag
      481 caatataaat tcctaaatct tgtaaagcct taaatttaaa ctgtaaaata ctcatatttt
      541 gtactgtatg actttctgtc aattctaaaa caagattttg ataaggaaaa ttttcatcat
      601 ctataatttt tactatatct tcaataaatg tgtcttctaa tatttgaaca gctgaaacgt
      661 ttacactcac tttaaaaaaa ggatagtaat caatccattt cctaaaagta cgtaatgcca
      721 ttcgaagcac ccaagcccct acaatattta tcatatcatt ttcttctaat attggaataa
      781 attccaaagg agaaattgct ttgcatttgt cattagtcca ccttaaaaga acttctacac
      841 ctattatttt atgactttct gtatctactt gtggctggaa tcgaagacta aaccccctaa
      901 aatcatcatt aattgatgcc tttaaatccc gcatcatttc taatgaatac attttatttt
      961 ttaatatttc ttgggagaaa aaaacaagcc tgtttttacc atgctctttc gcatattgta
     1021 aagagtaact agcacactta taaagttctt ttactgtatc tccatgtttt ggatatatta
     1081 cacaaccagc agaaattgtt atatttaatc catattttct ccatagttga aggtgtataa
     1141 tcatattctg tatttgatta tatagagtta gaatttcatt ttcttcaaca ttatcaacaa
     1201 gaatgcccat ttcgtcccca tcaagtttat aaagttcagc attatctggt aaaatagatt
     1261 gtatagactg tgctagtgtt tttattatat tatctccaaa atccctatca tacatctcat
     1321 taacattttt aaaatcgtca atatctaata atactatgca taatttttca atcataggat
     1381 ttgaaatttt atcttcaaat acactcataa attcatgata attcagtaac tgtgtcaatg
     1441 aatcaatctt attttgttta cctaattgcg tcattattcc tgcaaaaata cttggttctc
     1501 caaattcatc acgcattagt tgacctctac atcttaacca tatatattct ccactacgat
     1561 ttttagccct aaattccact gtatgataat ccatctggtt tttacctatt tcagtgtttg
     1621 acttataaaa acgattccag tcttctggat gtacaatttt tttccagtaa acaagcggat
     1681 tttcaacaat ttctcctggt aagtcaaata attcaacttg tgatggtgaa tatcgaaata
     1741 cgcctgtttt catattgcaa atgtatatat attcatctgt acctctaatc aaagcatcat
     1801 aaagcaatgt agaattataa tcaaaattgt ctcttgaaaa attaaaaact cctttttctt
     1861 ctttattatt tgatatttca tgaaattcat taatcttaat tggtttattt aagcttgttg
     1921 agtttgcaat atctattgat tgatgcatat gaacaactgt gccatcttgc catgtaatta
     1981 aactatcata attttcaaaa acacgattta acttattaca tttttcatac cactttatta
     2041 atactccttt tttatcattc tttaataatt ctaaaacttt acaaaaacta caagtagaat
     2101 ttttttcagg atataatact tgccaacaaa cttttccttc tggatctaaa atattatatt
     2161 cttctttcat ttttttgttc ataaaaataa tttcattagt atgaatattt gtaacatata
     2221 tattaatctg tagttgattt aatataactt caaaattatg tttgttcata tacttatctc
     2281 ctccatagtt actgctcttt ataacaacat tgttaaaatt attcttttta gaagatttac
     2341 ttataactta tacatataca ctaaatacct taatttaatt agaaagttta ttacttcata
     2401 ttttaaatta taattgataa aaaaaaatac agcaatatag tattaccaat
//
LOCUS       DAMJWU010000216         2398 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMJWU010000216 DAMJWU010000000
VERSION     DAMJWU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.222"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            330..2225
                     /locus_tag="PC460_003699"
     CDS             330..2225
                     /locus_tag="PC460_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF2602542.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1048 a          242 c          441 g          667 t
ORIGIN      
        1 agatagatat taatttagtg atatatattt aagtaataag agtaaagcaa aaattagtgc
       61 atagtagtat ataaaaatgg agtttgaata cttttgttag tatatgattg tttagtagaa
      121 aatcactata aaataggatt tctatctttg agcataatta tagagagcct aagtaggggg
      181 aagggtttca agaagatatt attagtgttt gtgttagtaa attatgtaaa cacataaaat
      241 atattgaaaa ataatattat atataatatt atttatgtgt gggaaatacg aatataaaaa
      301 ataaaaaata atattcggag gacataaata tgttaagcaa caaaaagaga agtatggcaa
      361 tagtaatggc aggtgctaca gttatgagtg cagcagcacc aatattcgca gacaataccg
      421 tgacagaaaa tgttgataaa aattacacag taagtgcgaa agattctgct aaactaatcg
      481 aagaggttag aaaagcatta gaagttaagt ttgaagatac aaaagcagga gcaaatgtaa
      541 atgatagagt ttacgatata aaagtagaca atgtaaactt aactaatgca actcaattac
      601 aaaataaaat aaactcttta acagaagggc aaagtttaaa agttactatt caagataaag
      661 ggcatcaagt gttaggtgga aaagtagttg actataaaat tgaaaactac aaaactgctc
      721 aagaaatagt agatgcagtt aatgcttaca atgcaacttt agcagaagat tctgataaca
      781 agttaacagc aactataaaa tctacaaata cagttgaagt aaaaagagca aaagattcag
      841 caaatgttat tacattaaat gtaggagacc aacatttaga tttctcaaaa gtgataacat
      901 cagaagaagg tacttttgaa ggttatgaaa agagttatag tgacatagat tcaaaagaat
      961 tacatacgat aacagttaaa aacgcagatt tacaagacat atcagcagaa gaattatttg
     1021 atggaattag attaactaca ttaggaagag aaatagttaa taaagttaaa aatggatatg
     1081 ctttaacatt tgaaaatgaa gcaatcctta ctcaagaaca agaagatagt gatgataaag
     1141 ataaaccaga aaaatcaagc tttgatatag ttttaagcaa ggcaaatgaa aagccagaaa
     1201 caataagtgt ttcaagtaaa aaccataagt tagttagaga cttacacaaa gtattaactg
     1261 atgtaaaaga tggaaaagaa ttaaaagtag aagttttatc tggtgattca agatttacaa
     1321 cagcagtaga agttagtaaa gagagattta aagatggaga agcagaagct ataattttag
     1381 ttggagaaga tgctatagtt gatggattag catcagcacc acttgcctct caaaaaaatg
     1441 caccaatatt attatctaaa aaagattcac taccatcaga aatagaagct gaaatattaa
     1501 gagtacttgg aagtaatcta tcttctaaga aaatatatat agtaggtgga gaatctaaag
     1561 tatcaaaaga aactgaagaa aaactttcta aattaggtgt aagtaaagtt gagagagttt
     1621 ctggagaaga tagatttgaa acttctttag aaatagcaaa acaattaaaa gatacattta
     1681 agactgcttt tgtagtaggt ggaaatggag aagctgatgc tatgagtata tcagctagag
     1741 ctgctcaatt tggtgctcca ataatagtta caggtaacga attagatgca aatgctgaaa
     1801 aattattaaa aggaaaagaa ttagaaatag tgggtggaga aaattctgta tcaaaagaag
     1861 ttgaagacaa attagttgat atagatttaa ataataaagt tgaaagatta gctggagaaa
     1921 atagaaaaga tactaatgct aaagtaatca ataaatacta tgcaggtgca actaaagcat
     1981 atgtagcaaa agatggttat gtaggtggaa atggacaatt agttgatgca cttacagcag
     2041 caccacttgc agctagttca aaggctccaa tagtattaac tacagaagaa ctttctaaat
     2101 cacaagaaga agtagttgag ttaagactta agaatgctac taagttagta caaataggtg
     2161 aaggaatagc taaaaacgct attgaaaaaa tagcagaaaa aataaactta tttactaaga
     2221 actaataaga tataggaatt aatattaaaa gtttatttag ataatatatg attatacaat
     2281 gcgttaatat gtaagacata gtgtagatat tataaaatat acaaataaca agataaaata
     2341 ataaaaataa ggtgtctcat ttagaattag attgagatac cttattttat tttttgat
//
LOCUS       DAMJWU010000217         2275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMJWU010000217 DAMJWU010000000
VERSION     DAMJWU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.223"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(131..1423)
                     /gene="brnQ"
                     /locus_tag="PC460_003700"
     CDS             complement(131..1423)
                     /gene="brnQ"
                     /locus_tag="PC460_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2602543.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIISPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          750 a          370 c          253 g          902 t
ORIGIN      
        1 tttttaaatt tatcaaatag gattaataat gttcttttat ctacaataat ttaataattc
       61 ttttaaatat aaaaaggaat tgcctatata atttaaaggc aattccataa ttatcaatta
      121 gtttttatta ttatgcagtt ttactgcttt tagatggaat aaagtttcct attattcctg
      181 ctattacaac tggaactatc cagttaaatc ctaatgaagc aaatggtaag ctattaacta
      241 gaggaatttt tacaccaaaa cttgttgcaa ctgttaatac acttactaat aatgccatgt
      301 atgcagcaca tttgaacacg ttgtcatttt taattttatt actaaataat gctagtataa
      361 ctagagttat agttgctgga tataccatac ttagtatagg agaagagaat tgtattattg
      421 tacttactcc aaagtttgat atgattgcac taaatacaca aactactata actatatgct
      481 catactttaa ttttccatct gtaagtgttg agaaatattg accagctgct gaagaaagac
      541 ctatagctgt tgttaaacat gctaatgcaa ctataatagc caataaaatt ttacctgtag
      601 ttccaagtaa agctgctgtt attgcaacta ttaaagctgt ttgttgaaca tcttgtccat
      661 attgttgaga cacagttgct ccaagataac ataatccacc ataaactaat gccattccta
      721 ttactgcaat tacacctgca cttattgtgg ctttcatctt ttctttctca cctgtataac
      781 ctttagctat tactgacatg attataattg atgcaaatac actagctgcc aatgcatcca
      841 ttgtttgata accttgagtt ataccttctg caaatacatt ttgtattaaa gaactgtctc
      901 ttatttctcc taatggagat atgatacctt taactattaa tactgctaaa gctataagta
      961 aagctggagt taatattgac ccaactatat ctacaacttt tgatggtctt attgttaaaa
     1021 ccaatactag agcaaagaat actatagaga atattactgg gcttattcca gaacctattg
     1081 taggtaatat acccatttca taagttgttg ctgctgttct aggtattgct aaaagtggcc
     1141 ctatacagat taccattgca catcctaatg caatacttaa tcctttgcct gctcttgaga
     1201 acatcttcga tgtgtctcca ttgaacttag ttgctgctat aactgctaat aatgccaaac
     1261 caccatctgc aaaaaggaat cctatgaaag ctattagcca actacttcca gataaaactc
     1321 ctagatatgg agggaagatt aagttccctg caccaaaaaa cattgcaaat agtgcaaatc
     1381 caattacaat tacatcttta aaattgattt gtttctcacc catttaaagc acccccattt
     1441 gaattttact accttttact aaaaaataag tccaatctct tcattaaatg aaaacctttt
     1501 gacgagattt tatttaatta tacatttttg acttaaattt gtcaattatg tttcataatt
     1561 tataaaatct tcacaatatt taatacaact ttcttaatta aacaaaatca acctgtatac
     1621 tttaaacaca gttggttttt tgttttttct ctaatttttc ttgataaaat taattattct
     1681 gaataattaa aatattaaga ttttttagat tattcgattt gttattaagt gtattgcttt
     1741 atatactgta tatatcactc tcctttttta cattcatata atctttttaa ttcaatttaa
     1801 attttaaata ttttgtgttt attatataat ctgaaaaaaa agcagtcaat agcattttta
     1861 atttttttaa ttttcaaaat tatgattctt aaaaaatcgt tttccttttg tcatagcacc
     1921 tatttacgtt aattcttgta gtattttgta tttattttgc taaattgaca gttaacttta
     1981 ttcgtttttt attctattta tgtagatttg cctcttgttt tttagtctta aaatcatttt
     2041 ttctgaaaat tccttattat gatttatcaa cttatttttc ttcatatcaa ttactttcta
     2101 acttccaact tacttaaact ttgcagttgc tagtattcac gtttacatac ttatctgtca
     2161 taattaacta aaaattctta atatatctct caatgaaaac aatttaccca tttacttaat
     2221 acatttatat aatcatatgt cttaaatttt gttttattat tttattattg tcaca
//
LOCUS       DAMJWU010000218         2246 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMJWU010000218 DAMJWU010000000
VERSION     DAMJWU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.224"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(65..979)
                     /locus_tag="PC460_003701"
     CDS             complement(65..979)
                     /locus_tag="PC460_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF2602544.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRYYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            complement(1811..1993)
                     /locus_tag="PC460_003702"
     CDS             complement(1811..1993)
                     /locus_tag="PC460_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602545.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          820 a          280 c          282 g          864 t
ORIGIN      
        1 gtacagttaa ctgtaccttt attttatcta aatatgcaaa gattatttta aaagtaattt
       61 ttaattacat gtttttataa ctctccaata ttctaatata atgatttgcc tctcttaaaa
      121 catggtctgc taaaagtggt aatattatag attttatctt acactgttct attcctgatg
      181 ctcctgcttc tttaaaatct ttaaactcaa cagtttcatt tagagtttct tctgtaatgt
      241 tcttgatatt agaatcagtc atttcgtttg ttttttcaat taattcatta aattttatag
      301 caaaatcatt tgaagtattt attagttcac cttctgaggg gtctagtaat cctctcataa
      361 acagagcatg ttccatcata atatggtccc agaataattc tatttcttta gcgttttttg
      421 actcaatatc tattttattt tcaaggtcaa ctacataata acgatataaa tttgcttcat
      481 gtattatatg ttcaagaagt agagggtagt ttgatgtaaa tatagtacat gatagtacac
      541 catctaagac tctttcttta aaattaataa gcccatcaag taatcttatt gcatcagagt
      601 taagttgagc tacatagttc actaagtctt gaccaacttg tgggtttaca ccactttgta
      661 gatttaattc tcttgtagtg atgtttttgt ttatttctat ccctgtaaac tcttctgttt
      721 tttgttctgc aactgatgtg agagtagtta caagctcttc tgaatataat atatcaggtc
      781 taattatacc attactagca ctaacagtgt atgataataa atcttcaaat tgctttttat
      841 agtggtcagc ttccatagca agattataat ttttatttgt gaatcctgct tctaaaaaaa
      901 gagcatgctc tttcataatt cttgaaaaaa ataaatgtaa ttctagtgat aaaataacat
      961 atttttgatt atctatcata atttcctccc tttagaaagt atcctattta taatacttta
     1021 ttcgtaatat ttaaatttat gtatattata aataaattaa ctaaattcaa ttgctactaa
     1081 tatcaacagt ttttattaag ttatgggcga aaaaattatc ttactgtgtt tcttattaga
     1141 tatagatata atgatttaag gtacttgttg gctattattt attaagagta caagtaatac
     1201 tagaaatttt tatgagaatt atgtaattta caagtattat tttgatacaa aacaaatgtt
     1261 ttttgtaaaa caagaacttt tatagtaaaa aatgaataaa tatgaagcaa gcataatttt
     1321 ataccacaac aattattaat tctaatagct gcacttaatg ctttaggtat gggatttaaa
     1381 aagtataaaa aattaagtga taaatatatt ccattatatt gcttgtgctt ggagtagtat
     1441 tttctgtata gatgttagga tttaatccaa cttctatttt acaggggatt ctatgctggg
     1501 gtgtttcaat tggattaaat taagtatgca aacaatttca agacaatgat aaataattgg
     1561 atgtgtgata ggaatatcaa caaaaaacat tattaaaaag aattatttta tttataaaat
     1621 aaaacaagta ataagaataa ataaggagca actaaaatga tttaaatata ctagaattaa
     1681 aaagagaatg taaagttgtc tagtaatgga tgtaaaatat tttaaacgat aagctatgaa
     1741 attgatatat taaaatgggt aagaagaata ttatatagga ttcttcttac ccattttaat
     1801 aaatttaatt ttatgccaaa ccacattcgc atagtagaat catcactcta tggagacata
     1861 actctacaac atcttctcca tattttttaa taaaatcacc aatttgagtt tctatgaatg
     1921 aatagtattc ttttaaacta gcaatatatg cagtcattat atgaacacat tcatcaatct
     1981 tatctttaaa cacaactaat ccccctaaaa aacgaaaaat aaaaatattc agtaaatagc
     2041 agatattatt tcactcataa atctatatag tatgatatat tgcttagata tattttacaa
     2101 gtggatatgt aaaactggtt attataatcg caaaactgga taaactttca ttcttaaaag
     2161 aaaatttatc cagtttaaag tttaattatg aatatttgag ttagtagata tttaatataa
     2221 attaatataa gtgtaataag aatata
//
LOCUS       DAMJWU010000219         2035 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMJWU010000219 DAMJWU010000000
VERSION     DAMJWU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.225"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            148..546
                     /locus_tag="PC460_003703"
     CDS             148..546
                     /locus_tag="PC460_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602546.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILFIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    981..1244
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1395..1946
                     /locus_tag="PC460_003704"
     CDS             1395..1946
                     /locus_tag="PC460_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF2602547.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          870 a          185 c          303 g          677 t
ORIGIN      
        1 ttgaaaaatc ttaaaaaaag taaaagttat gataataaaa ataatattaa agataataag
       61 atatataaga aacttgaaaa aatttgtaat ttttagttat atatatatta taatgaacta
      121 gttaaaaatt tttatgaggg ggaaaatatg aataagaaga aaaaattgat tgggatagct
      181 atatcttttc tacttataat cagcattata aatccattac aagtagaagc agaagctatt
      241 attgagtcta gtgtatacat ttcttcaata aaagagaatt atacatatga taatactaaa
      301 tttaaaacta ttaaatctaa aagtagacat aaaagtagga aatataataa aagtaaagta
      361 aagttgaaaa aacgaagtat aagaactaaa aagtatggat atcaaactat aaataaatat
      421 aaaagacaaa acgcaagaag acataatatc ctaacaaaat taatattgtt cataatagtc
      481 atatttttgg ttatagtttt agtagtgcta atatttatta aaagaaagaa gaaaaaattt
      541 tactaaaaat aattaaaaga atatcatttt tttaatcatt ggtatctatt taataatatg
      601 atgtaattaa aatggagtaa tattctgttt caaaggaggc atataaatac aggaatgagt
      661 attaaatgat ggttaattaa gtaaatttat tgataaatgt acttaattaa ctttgaagaa
      721 tttaattcct catgaatcaa aatataaaat tacatcaaca gttattcaca aaattaatat
      781 aagaagaatt agagccaaag agcagactat tagaccatta aagtaattat tcatagaatt
      841 ttgtatatga aaagaaatgt atagtataat ttactttttt tataaaaaat agattacaaa
      901 atatattttt tataaaaagg tattgacatt ttttagaaaa aagaatattg ttattttaaa
      961 acaaaattca atacaataaa ttaaatccta tgacaaggaa gagtagttaa atttaggttc
     1021 agtatagaga gctgaggatg gtggaatctt agcaagaaaa aatttgatga atggccctta
     1081 gaggaataaa ttgaaatcat agttttgaga gtagacttta tcgtcagtcg cacgttatag
     1141 cgaataaggt atgtaagtac ctgataatga gatgtatgaa aattgaattt tatattaatt
     1201 tttatataat ttaggtggca acgcggataa tctccgtcct attagtttag gacggagttt
     1261 tttattttac tgaaaattta aaagatagaa ggtggtgaga aagatgttat gaatttcaaa
     1321 agctaaagat gatgatatag taggttggca aaaaaataaa ttgaaaatta aaaaaatagg
     1381 aggaagtaca aaaaatgaaa acaaatacaa aaaaattaac tttaaatgca atacttttag
     1441 cgatgggatt attaatacat caaatcacac cagctatagg attaccaatg aagccagatg
     1501 taccactagc aatgttattt gtaatattgg ttttaaatag agatgattat aaaacttgtt
     1561 tgatagcggg tatagtaaca ggaatattta cagcacttac atcaagtttt ccgggaggtc
     1621 aaattcctaa tgtaatagat aaaactctta cagcaaatat agtattttta ttaatgaata
     1681 ttagttataa tatgcctttt ataaaaaaat tagctaagaa aacacaagat actgtagtag
     1741 ttgcaataat aatgccaata ggaactcttg taagtggaac tatcttttta ttagcagctc
     1801 aagctatagt tggtcttcca ggggcttcat ttacagcttt attcttagca gtagtattac
     1861 cagctgtttt aataaatcta gttgcaggaa tatttttatt caagattgtt agtctatcaa
     1921 taagaagagt tagttatcaa gcttaaacac gaaataatat tttactttat aaagcattat
     1981 aataactaaa taacataaga ataaagtaga ttataactat tttattctta tgttg
//
LOCUS       DAMJWU010000220         1975 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMJWU010000220 DAMJWU010000000
VERSION     DAMJWU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.226"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            226..1902
                     /gene="bclA2"
                     /locus_tag="PC460_003705"
     CDS             226..1902
                     /gene="bclA2"
                     /locus_tag="PC460_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF2602548.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGVTGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGENGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVTQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
BASE COUNT          737 a          356 c          520 g          362 t
ORIGIN      
        1 gatgggaaat gagtttttta attgtattaa taattgtaaa agagtactaa taattgtaaa
       61 agattttaca aaaaagtaat gaaatttaag aatcaaagca tatatgttga tataagcaaa
      121 tagatacatt atcacataag aaattgtcga ttcatataat tatatagagt aatactcaat
      181 atttatagag tattactcta actttaaaaa aggaggatta attatatgag tgatatttca
      241 ggtccaagtt tatatcaaga tgtaggtcca acagggccaa caggtgctac tggtccaaca
      301 ggaccgacgg ggcctagagg tgtaactgga gcgaccggag caaatggaat aacaggacca
      361 acaggaaata caggagcaac tggagcgaat ggaataacgg gtccaacagg aaatatggga
      421 gcgactggac caaatggaac aacaggttct acaggaccaa caggaaatac aggagcgact
      481 ggagcgaatg gaataacagg tccgacaggg aatacaggag caaccggagc aaatggaata
      541 acaggaccaa caggaaacaa aggagcaacc ggagcaaatg gaataacagg ttctacagga
      601 ccaacaggaa atacaggagc gactggagca aatggaataa caggtccaac agggaataca
      661 ggagcaacag gagcaacagg accaaccgga ctaactggag caacaggagc aaccggagca
      721 aatggaataa caggaccaac aggaaataca ggagcaaccg gagcaaatgg agtaacaggt
      781 gctacaggcc caacaggaaa tacaggagca acaggtccaa caggaagtat aggagcgact
      841 ggagcaacag gaacaactgg ggcaacaggc ccaataggag caacaggagc aacaggagca
      901 gatggagagg taggtccaac aggagcagta ggagcaacag gtccagatgg tttggtaggt
      961 ccaacaggcc caacaggccc aaccggagca accggagcaa atggtttggt aggcccaaca
     1021 ggcccaaccg gagcaaccgg agcaaatggt ttggtaggtc caacaggagc gacaggagca
     1081 acaggagtag ctggggcaat aggtccaacg ggagcagtag gagcaacagg cccaacggga
     1141 gcagatggag cagtaggtcc aacaggagcg acaggagcaa caggggcaaa tggagcaaca
     1201 ggcccaacgg gagcagtagg agcaacggga gcgaatggag tagcaggtcc aataggtcca
     1261 acaggtccaa cgggagaaaa tggagtagca ggagcaacag gagcgacagg agcaacaggg
     1321 gcaaatggag caacaggccc aacaggagca gtaggagcaa cgggagcaaa tggagtagca
     1381 ggagcgatag gaccaacagg cccaaccgga gcaaatggag cgacaggagc aacaggggcg
     1441 acaggagcaa ccggagcaaa tggagcaaca ggtccaaccg gagcgacagg agcaacagga
     1501 gtgttagcag caaacaatgc acaatttaca gtgtcctctt caagtttagt gaataataca
     1561 ttagtgacat ttaattcatc atttataaat ggaactaata taacttttcc aacaagtagt
     1621 actataaatc ttgcagttgg agggatatac aatgtatctt tcggtatacg tgccacactt
     1681 tcacttgcag gatttatgtc aattactact aactttaatg gagtaactca aaataacttt
     1741 attgcaaaag cagtaaatac acttacttca tcagatgtaa gtgtaagttt aagcttttta
     1801 gttgatgcta gagcagcagc tgttacttta agttttacat ttggttcagg cacgacaggt
     1861 acttctgcag ctggatatgt atcagtttat agaatacaat agtttatatg atgaaaagat
     1921 tataaaataa taaacaatat gatttcatat accaataaat agaataatat ttaat
//
LOCUS       DAMJWU010000221         1854 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMJWU010000221 DAMJWU010000000
VERSION     DAMJWU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.227"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            161..508
                     /locus_tag="PC460_003706"
     CDS             161..508
                     /locus_tag="PC460_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2602549.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(658..1707)
                     /locus_tag="PC460_003707"
     CDS             complement(658..1707)
                     /locus_tag="PC460_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2602550.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
BASE COUNT          629 a          284 c          249 g          692 t
ORIGIN      
        1 atatcttata aggattgtat ttttctaact ataaaaatag tattatactt atatttaaaa
       61 ataaaagaat tttattccta ttgtgtaaag tgagaatact attttcttat attggtataa
      121 taatagtaaa tctataatat agaaaagagg tatttctagt atgcatgaaa ataaaaaaaa
      181 tctattagac ggaagcttag ctcaatataa tgatacagca gttccaaaaa taccagtatt
      241 tgctggtaac gatataactt cagaagttta ttattttaaa cctaatcaag ttcttaatgc
      301 tcatagacac cctaatggtg aacaaatatt tgtgttccta aaaggagaag gtaaaatgaa
      361 acttggtgag catgaatgtg atgtaaaaaa cggagatact gtatttgtac caacaggaga
      421 gtggcatgaa attactaatg gaagcaatga agaaatggta gccgttcaaa ttacaaaaat
      481 taatgctgga gcagaataca gaggatagat atataagtac agttagtact ttaatatcta
      541 ttttattata taaactttaa tctcaaatat aaaaagagtc ttttaaaagg ctctttttat
      601 atttttgtta attttcatta taaaaacaag aaaatatcta gctaaaacta atatatttta
      661 tttaatattt tttatagcat tgtttgcaaa ctcagtattt acgatatctt tataaggtgc
      721 ttccttatct agttctttag cttctttcat aacagtcatc aatttcttca aaccttcctc
      781 ttttaataca ggattttgag accatgcatc tattgattta tatctatcta caactgttgt
      841 taaatcttct aaagatatat cttcaaagaa aggttgcata gccttagcta cttctttgct
      901 agatgcttgt tgaatccata attgaccttt ataaacagca tttgtaaatc tttgaattaa
      961 atctttattg tttttcatgt atgatctcgt agtagagtat gctgtaaaag gtatttctcc
     1021 agaatctttt cctatagatg taactatata tccactacct tctttttcca ttgctgttgc
     1081 tgtaggttca aatgctgttg taaagtcacc ctctccacct gcaaaagctc cagccattac
     1141 accaaattgt acatcagttc ttacattaac attgcctgct tttagatttt ctccaatagt
     1201 aagacctttt tcttttagaa catattcaaa agtcattagt ggaactccac cttttctacc
     1261 acctaatatt tcggttcctt ttaaatcatt gtaagagaaa ttaggcattt ttttcctagc
     1321 aactagaaaa ctaccatctc tttttgtcaa ttgagcaaag ttcactgcat aatcgttttt
     1381 gttttgatta tatacatata tagatgcttc aggacccatt aaacctacgt ttgcttcacc
     1441 tgaaatcaaa gctgccatag ttttatctgc accttgggta tttataaggt caatctttat
     1501 accttcatct tcaaagaatc ctttcgtaat agcagcatac tgtggagcat aaaaaacaga
     1561 gtgagtaact tctgctatag ttactttatc cagattttta gacgaagaag attcatcttt
     1621 tttgtcatta gagttaaaat cacatccagt tagacatatt gtacttacaa tgatagagct
     1681 tgctattgcg actatttttt taaacataat aaatacctcc cataagacat acgaatagta
     1741 tatagtatta aatatttatt gtagttgtta caaaaatgta tctatactta taatatttct
     1801 acaaaaactt aataataatt ttcagtttta tgtttagttt tttcagtaat gtgt
//
LOCUS       DAMJWU010000222         1849 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMJWU010000222 DAMJWU010000000
VERSION     DAMJWU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.228"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            114..308
                     /locus_tag="PC460_003708"
     CDS             114..308
                     /locus_tag="PC460_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF2602551.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(410..919)
                     /gene="vanZ1"
                     /locus_tag="PC460_003709"
     CDS             complement(410..919)
                     /gene="vanZ1"
                     /locus_tag="PC460_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF2602552.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1239..1628)
                     /locus_tag="PC460_003710"
     CDS             complement(1239..1628)
                     /locus_tag="PC460_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2602553.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEGDIDD"
BASE COUNT          684 a          256 c          191 g          718 t
ORIGIN      
        1 tgcattataa taaaaagtaa atctaaattt gatacagttt taacaatcat tattttcaga
       61 aaagtgatac tattgaatat attaagaata attagagatg gaggaactaa gatatgagcg
      121 taattgtaat gtgtagtcgt tgtggaaaag aaatagataa atatagaaat aaatatataa
      181 cttattatga gaaaaatacc aatgcagaaa ttaaagtttg tataaattgt gcgttagatt
      241 tattagaaaa aacaagagaa aatcaagaat tgaacataca agagtttcaa gatacaataa
      301 ttctttaata atttaggaaa gcacctgtat aggtgctttt tatgtgtaaa taattttata
      361 tgtaactgta tcttacgttc tattttaagc taaacatata tacttatttc tataagtttg
      421 caaaaataac taatcctaaa aataatacaa caataattgt cacaatcaat gccaatacat
      481 tgataatttt tatggtatta ttcttaaata ttttcgataa tagcataaaa acagctattc
      541 ctatgattcc tcctagtgtg tttcctatca aatccgttat atcacttgca cctagtgcaa
      601 atatatattg aagtgtttca aatgccaaac tagttataaa aataggtata acttttttta
      661 taaagctcca ttcttctttt agcatacaca catatatccc aaatggtaca aatatagcca
      721 catttaatat aatctctgat atatctactc tattattaat tataagtgac ccagcaaaag
      781 gtactaagtt tatgcttcta agattcatgg tttcaaggga tgaaatatca aattgcaact
      841 tgaatagtat tatccatgtt aaaataatta tgtaaactat aaataatcct tttgtaatat
      901 tatgttttct agatttcatt tattacctcc ttactatatc attgtattat tttattaata
      961 caatgatata gtaaggtaat ttatctgtca actatttctt ctaaaattat attcctttta
     1021 taatttcaat atctttatca tatttacatg atttaagcta ttgtagtatt tcatatatct
     1081 ttaaatttta attggtatta ttctctatat tttttactat caaacactaa ggaaataatc
     1141 acttttcttt atataatgtt ctaccatcac ataaaactaa cattaaatca aaacgctact
     1201 ctcaattgtc tcaaactaaa aagatactga taactttatt agtcatcaat atctccttct
     1261 ttcaaatttt taaagtattc ttctaacttg tccaagctct tactatctaa aacttcatca
     1321 tcatgtaatg ctgaaataaa actcttaaat gaattgttat gcataaaact aaaaaaactc
     1381 tttgtctcaa cttttaagta atctttctct tttatagcta tagtataatg cgtacatcta
     1441 tctaccttat cagtagttaa aaagccctta tctactaaat tttttaaaaa tgtcaatgtt
     1501 gtgctctttt tccaatcgta tttcaacttc atgtcttcta tgatatctct tgaggctaat
     1561 acatcatcta ctccccaaat atacttcatt agttttagtt ctgcatgtgg taatttatta
     1621 atcttcatta gtttacacct cttaccaatt tatgtataac taaactttat aacaataagt
     1681 gtatataatc aattagattg gaataaatat aaattccaat ttattctata taaaaataat
     1741 taataaaaaa gaagtactct actttagaga gtacttttgg attatatatt caaaaattta
     1801 tctaacatga ttagagtatg ttttatgctt tagtgtgata attttagtt
//
LOCUS       DAMJWU010000223         1799 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMJWU010000223 DAMJWU010000000
VERSION     DAMJWU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.229"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            99..1445
                     /gene="rpoN"
                     /locus_tag="PC460_003711"
     CDS             99..1445
                     /gene="rpoN"
                     /locus_tag="PC460_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF2602554.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT          829 a          161 c          318 g          491 t
ORIGIN      
        1 cccttttgca taaggtatat tttaaatgat agaatataca tatacaggag ggtttttctt
       61 ttataaaata agtttaataa aattgagagg tgagcagaat gaattttaat aatagtctag
      121 aattaactca gtctcagaag ctaataatga ctacacaact taagcaatca ttatcaatat
      181 taaatatgag taagttagag ttagaagaag agataaaaag agaagcagag aataacccat
      241 tagttgaagt cgagaaaagt ggggagataa attgggaaga gtatataaaa gacatggata
      301 ggtcaagacg tttagataga acagagataa gctataatcc tgataatgaa gttaatctag
      361 aaaatttagt taaatattcg tcaaatttat atgaagattt aaaatttcag ataagcctgt
      421 ataaacttac agacaaagaa ttggaagtct gtgagtatat aattgatagt ttagatgagg
      481 atggatattt aagaactgag gaaaaagtaa ttgttgatac cttaaagata gatgaagaac
      541 tttttgaaaa atgtttaatt agtgttcagc agttggaacc tagtggagta ggggcaagaa
      601 gtctttcaga atgtctgatg atacaaatgg ctagtttagg aatatataat gaaattttag
      661 aggaaatcgt tacaaaagac ttaaatttaa ttgcaaataa taaatacaaa gagataagta
      721 aaaagtacaa tatgtcatta caaaaatgtg ttgaactaat aaacataatt aagacattag
      781 acccaaaacc aggaagaact tgttcagtag agaaaagtgt atacattcaa cctgatgtaa
      841 cagtagaaaa aattgatgat gaatttattg tatatttaaa tgaaaaagat tcttatcaaa
      901 taagaatcaa tagttattat aaagacatat taaaaaattc acagtctgat gaaagtgcaa
      961 aagaatttat aaaagaaaga ctaaattcag caacaggact tatgaaaaat atagagagta
     1021 ggaaaactac agtacttaag attgcagaag agatagtaaa agctcaagat gaatttttga
     1081 gaaaagggac aaaatatata aaacctttga aaatgaaaga catagcagaa aaattagaat
     1141 ttcatgaatc tactataagt agaggtgtaa atggtaaata tatgttaaca ccttttggtg
     1201 tatatgaatt tagatacttt ttcagtagtg ccattgagac tgagaataat gaaatggcat
     1261 ctagtacaag cataaaaaaa attataaagg aaacaataaa agacgaaaat aaaaagaaac
     1321 ctttaagtga tgaccatata tctaagttac taaaagaaaa aggaatcaat gttgcaagaa
     1381 gaacagttgc caaatatcgt gaagaattag gaatcccatc ttcaagtaag agaaaagaat
     1441 actaataagt aaaagacaaa tatcagtatg gtaaggggaa aaggctaatt aaaagatgaa
     1501 tgtagattgg gctaaaaatt ttatgaagta aaataataga atatggaaaa gtattaaaat
     1561 gctataagat attaaaaaac tggggaacat taaaacatta agatagcaaa gaacataaaa
     1621 aactaaagaa gattaaaaca ttaaagaaca ttagagcttt gaggaatatt agaactttaa
     1681 agaatattaa aatttataaa atataaaaat actaaaaaga attgaaaaac tggggaacat
     1741 taaaatattg taaaatgcta aaaaactaga aaacataatt aaataagtgc ttataaaga
//
LOCUS       DAMJWU010000224         1796 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMJWU010000224 DAMJWU010000000
VERSION     DAMJWU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.230"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            175..1503
                     /locus_tag="PC460_003712"
     CDS             175..1503
                     /locus_tag="PC460_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2602555.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          612 a          206 c          328 g          650 t
ORIGIN      
        1 tggtttttat aattttttta tatttaaaca tagctaaaaa taaaagatta cgaaagctaa
       61 gtagagttta ttttatttta ataaataata aagctatttt tctatatatt ggcataatat
      121 atgcttattt aactatgaaa ttaaaattaa ataggattaa tgaggaggaa aaaaatggga
      181 agtaatgaac tgaaaaagac tcttggcgta tcagcagcat tatcaactgt tgttggttcc
      241 gttataggag cgggcgtatt ttttaaacca caagcagtct atacattaac tggaggagct
      301 ccaggtcttg gtatattggc atggttaata gctggaatca taactataac agctggtctt
      361 acagctgcag aggtatcagt tgcgatacca aaaactggtg gaatgatggt atatataaaa
      421 gaaatttatg gagaaaaatt aggtttttta actggatgga tgcaaattgt attattttat
      481 ccagggatga tggcagcatt aggtgttata tttggagaac aagcatcagc actaattggt
      541 agtccatcac ttttacttcc aatagcaatt ggaattatag taatagttgc agggcttaat
      601 atgttaggtt caaaaactgg aggagtcata caaactgttt ctacaatttg taagttaata
      661 ccacttattt taataatggt tgttggattt attaaaggtg gtggaaataa tccaatactt
      721 acacctatgg ttggtgaagg attaagcctg ggtagtgtac taggtcaagt tttaattgct
      781 atattatttg cttttgatgg atggatgaat gttggtactt tagctggaga aatgaagaat
      841 cctggaaaag accttccaaa agctataata ggtggattgt cagttgttat ggctgtttat
      901 tttataataa atttagcgta tctttgggta ttaccagcta atgaattagc aaattatgct
      961 tctcctgcat cagcagttgc tgaagttata tttggttcaa tgggtggaaa aataatatct
     1021 gtaggtatat taatttcagt gtttggagca ttaaatggat ttttactaac tggttcaagg
     1081 gtggcatata ctttagctac agataagact ttaccaaagt atggtatatt ttctaaatta
     1141 aattctgctc aagttccagc aaatgcaata gcattagtat ctgttattgc atctatatat
     1201 gctttgtcag gtcaatttaa tttattaact gacttagctg tatttgctac ttggatattc
     1261 tatgtattaa cttttatagg ggttatgaaa cttagaaaga ctcatccaaa tataccaaga
     1321 gagtataaag ttccattata tcctattgtt cctataatag ctatagcaag tggtatattt
     1381 gttgtagtta atcaattgtg ttttgcaggt atgaaaacta ctatgatatc tataggaggt
     1441 cttgtaataa cagctatagg cttaccagtg tatgcttata tgactagagg agtaaaaaga
     1501 taaaccaagt agtttacaaa tgcttcataa ttaaaataaa agagactgtc tcaaatagag
     1561 ataagtctct tttttattgt taaaatggta aagatatgtt taattttaca aaaaaagagc
     1621 gttccatgaa ctaaaaagtt tattttaagg tattcatttt atttttagtt ttttgattat
     1681 aaaattaaac atctaaaaaa ttatttattt taaaatactt tattttaaag tgttttttat
     1741 caaactaagt ggtatctgta caacaaatat tatacatagc ttttatgaaa attaaa
//
LOCUS       DAMJWU010000225         1703 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMJWU010000225 DAMJWU010000000
VERSION     DAMJWU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.231"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            620..1327
                     /locus_tag="PC460_003713"
     CDS             620..1327
                     /locus_tag="PC460_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2602556.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          718 a          146 c          286 g          553 t
ORIGIN      
        1 ttattataaa tatttatttt aaaaaaatag aattattata tttatgataa taaatggtat
       61 aataaaaggt aaagtgtcaa atatgcacta aattaaataa gttaattagt atataggtta
      121 aagtggaagt aattaaaact tattaagtat tagaaaatga cttcttatta gagtaagtat
      181 ctaaaatgat ataaaaatta tgattactca ttaaagtgac tattatagag tggataagat
      241 tactcattaa agcgattact gtggagtaga taaaattact cactaaaata actatcatag
      301 agtggataag attactcatt aaagcgatta ctgtagagta gataaaatta ctcactaaaa
      361 taattatcat agagtggata agattactca ttaaagcgat tgctgtggag tagataaaat
      421 tactcactaa aataactatc acagtagata agattattca ttaaagttac tatcacagag
      481 tggataaaat tacacattaa aattgtctgt taaagaatga ataaaaaaga gattgctcaa
      541 tataagaagc atataaaaaa taaaaaatca aacgtataaa atttaataaa taataactga
      601 tagggaaagg aaagatagaa tgatatttac aaaaagtaat aaatacgata aggactttct
      661 catgaaaaat atgatgggac ctaattgtat aaaaatattg gaagaattaa caagtaagat
      721 aaaactagaa aagggaatgc gtattttaga cttaggttgt ggtaaaggta tatcttcgat
      781 atttttagca aaggaatttg atgctactgt atttgcaaca gatttatgga tagaaccaac
      841 tgaaaactat aaaagattta aagagtttaa attggatgat aaaatatttc caatacaggc
      901 agaggcacat gaactacctt atgcagaagg tttctttgat gcggtgataa gtatcgattc
      961 ttatcactat tttggaaaca aagaaggatt tttagacaat catatatcac ctcttgtaaa
     1021 agagggggga atacttgcta tggcaatgcc tggattaaaa gaagatttcg tggattgtat
     1081 accagatgaa ctcattccat tttggcaaga taatatgaac ttccattcaa ttacatggtg
     1141 gaataaactt tggtctgaat cggagtctgt gattgtagag aaatgtgaag ctttaaattg
     1201 tcatgatgaa gcttggaagg attggataaa ttgtgataat tcatatgcta taaatgacaa
     1261 aaaaatgatg gaagtagaga atggaaagta ttttaataca atttctttga tagcgagaac
     1321 taaataattg tgatgaattt ataaaagtat aagataagta gaaatataag ttgaatgaga
     1381 gtataagaag catagaaata taagttgaat taaagtagaa ggtaaataaa aatgtaagtt
     1441 aaataaagag tttatacaaa aatgattttt tagattattt atacccaatc gagttgctta
     1501 ggtcagttgg gtatttttat tttttaaagt taaattgcaa atactattgt gtgatatata
     1561 ttttaatact aaatgtttgt tttataagaa ataaactaat agaaaaaata gttttaacaa
     1621 tgaaagcaaa aaaggtattg atgaagatta aaaattttat agtaccattt tatatatttt
     1681 attatacttt aaatcaaatt tca
//
LOCUS       DAMJWU010000226         1637 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMJWU010000226 DAMJWU010000000
VERSION     DAMJWU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.232"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            206..1546
                     /locus_tag="PC460_003714"
     CDS             206..1546
                     /locus_tag="PC460_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2602557.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCV
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          643 a          123 c          263 g          608 t
ORIGIN      
        1 aaatttatat agttattatt aaaaaaagca ataaaaagtt taattcaatt tagcaaattt
       61 agtgatataa ttaaaaaaga aaagaaatat ggacttattt ggaataattt tgacaatcta
      121 tttactacac gtgtagagtc gtatataatt aaattataca taaaatataa aaaaataatt
      181 tagtaatacg ttaggagaaa gtattatgat aaagtcaagc ttattttgga atattttcat
      241 tgcgatatcc tttatgatag aatggtttct ttgtaagaaa atgctagatt atacaagtga
      301 gaaaaggatt tctaacaaaa aaataaactt ttgtgtggta ggtattattg tttttgctga
      361 cataatatat ttttttgatg tacctccaaa tataagagta attatatcta tgataattac
      421 tattgcattt tataaaatct catatgatgt ggggtttata aagtctgtat taatatcctt
      481 aatttattgg atgttactaa tcgccacaga tgctttgagt atatctttaa cagtttggct
      541 taattctata aatgacatga gtagtatggg gacgggagat atatacagaa ttgagtctat
      601 aatattggga aagatacttt tgattgtgtc tttgctttta tatagagcct taaagataaa
      661 acttaatata gaaataaata aaaggacatt attttatata gttattccta tagtcgctaa
      721 tatagcatct ttttttgtaa tttttgaata catatttaat ccagggtctg gtagcttaat
      781 acataatttc caattcgtta tgatttctgt tgtattgctt atatcaagta tcactttagt
      841 tttaattgct aaaaaattac agcaatataa tactttagtt gtagaaaata atatgattaa
      901 aaatactttg aagtatgaaa atgagtatta tgaagatata gaaaagaaat atttaaaaac
      961 tagattacta agtcatgata tgaaaaatca catcttgtgt ataaatgcta tgattaaaaa
     1021 aggtattgat gttacttctt atttaaataa tttgcaggta gaaattcaga gtaatgactt
     1081 gttatttaat actggtaatt ttatattaga tgctatatta agtgaaaaga aagaggtttg
     1141 tgataagaat gatatagaac taaaaatagg aataaatttt agttgttgtg aagctgttga
     1201 attagtggat atatgtcata tattttcaaa tattttagac aatgcaattg aggcatgtaa
     1261 taaaattgat agtggaaata gagttatttg tgttaaaggt gatgttatgc aaaattttta
     1321 tttaataaga gttgaaaata ctaagatgaa taagattatt actaaaaatg gagatatttt
     1381 gacagacaaa aaaggttctc ttcatggact tggaataaaa agtgtaaaaa actctgtaaa
     1441 taagtatggt ggtaatgttg taattgaata tgatgataaa aaatttgtag ttaaaattgc
     1501 tattccactt agaaatgtga agaaagcagt taatcatgtg atttaaatgt atataaattt
     1561 gatgtgtaga aatttaatat ataagaatta atgtataaaa gtgatgcata gaagtttaat
     1621 atataagaat taatgta
//
LOCUS       DAMJWU010000227         1590 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMJWU010000227 DAMJWU010000000
VERSION     DAMJWU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.233"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            184..429
                     /locus_tag="PC460_003715"
     CDS             184..429
                     /locus_tag="PC460_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF2602558.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLI
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(647..916)
                     /locus_tag="PC460_003716"
     CDS             complement(647..916)
                     /locus_tag="PC460_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2602559.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT          642 a          219 c          176 g          553 t
ORIGIN      
        1 tataaaacta tattatgatg taattaagtg actcagtgat ttagaggtgg taaaatatat
       61 atatatgaaa gagcataaat aaattgatat aataaaaaat taaatattaa atattaacta
      121 taataaaaaa atatctggta ataggtataa tataaaaaga atataccaga aagaaggtga
      181 agtatgggag aactacaaaa aattccaggg gtagggaaag caacagaaaa atctttaatc
      241 atgttaggat atacaactat taaatcactt aaagatgcaa acccagcaca aatgtatgaa
      301 aaggaatgtt taatacgtgg acagcacata gacagatgtc agttatatgt atacagatgt
      361 gcagtatatt ttgcatcaac tgaaaatcca gaaccagaaa aattaaagtg gtggtattgg
      421 aaagattaaa aaatcaaaaa agttagacat aaaaaagagt tgaatatccg ttttaaataa
      481 taaaaactaa tactcaactc ttttttagtc gtaaatttta tgtaaatcta tttgtatggc
      541 taaatatacc catcaataaa tgtataaata taccaatcaa caaatgacta aacatactac
      601 ctaacaaagg attaaataaa ttacttaaca aagcattcta aaacaattaa ttagaaactg
      661 tttcttcaag ccattttcta cattgctcaa tatttaccat agggtcaaaa ccttggtctt
      721 ttcccttatt taaaagcata tcacaaggga aagtatcaca ttcaccacaa tgattaagtt
      781 ccttactttc acagcaagtt ttaaccttac attctccacc ccaaaatggt ttttccatag
      841 ttgggcaacc agtgcaattt acttgttctt tttttgtaca actgtcacaa cagacaccac
      901 atcttgattc aaacatgttc aaacatcctt tccattattt aaaaatatta taaaatgatt
      961 ttagatgaat acaaaagtgc aaatagttga ttttaggtca tctaaatgtt aaacataaaa
     1021 ttattagtac tggtaaattt ataaaaacta ttccaaaaat ctttttgaat atttcaaaac
     1081 acaataaatt acttcaaatc cctttgaata tattttacag cattatagat tgctttaaat
     1141 ctctttgaat attctacaag actatagatt agtttaaatc cctttgaata tattttacaa
     1201 cattatagat tgttttaaat ctctttgaat attctacaag actatagatt agtttaaatc
     1261 cctttgaata tttcaaaaca ccatagatta ctttaaatcc ctttgaatat tctataacac
     1321 tataaattac ttttaacttt tatataacca taaagttttt atctggcact ttaaatggag
     1381 ttttatatca aatttgcctt ccatcgaata accaaactta atttaattac acattccaag
     1441 agtcaaatat tcattcaata tatttgataa ttctatgtta tagctataat taaatataga
     1501 ttattaaagt taaatattta tttaatatat tgaatgattt tttacctata tttaattaag
     1561 tttcattata taatataaaa attgtttttg
//
LOCUS       DAMJWU010000228         1504 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMJWU010000228 DAMJWU010000000
VERSION     DAMJWU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.234"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            133..414
                     /locus_tag="PC460_003717"
     CDS             133..414
                     /locus_tag="PC460_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602560.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            678..932
                     /locus_tag="PC460_003718"
     CDS             678..932
                     /locus_tag="PC460_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602561.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            977..1300
                     /locus_tag="PC460_003719"
     CDS             977..1300
                     /locus_tag="PC460_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602562.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT          640 a          108 c          239 g          517 t
ORIGIN      
        1 atattaaaga agaaggaata taaaacaaga ttagaaaata atagtgttac tttcataata
       61 aatacatata aagatatttt taagaataaa atttattatt aaataaaaaa taaaggagag
      121 tgatttaata gtatggcatc aaaacaaaca ccaagaaaca atttagaaaa gtttttaaga
      181 ataagagtat tatcacatac aggcgataaa atacatataa agttacctgt aaattttgca
      241 aagagaatga ttgagaataa tgccttggat ttatttaatg gaaaagatga tgtagtagat
      301 ggtaaaaaag ttactgaaat attagttaag gcatttgact atgatttaac tggagaagta
      361 gttcacttgg agagaaaaaa tggagacatt attaaagttt caattgtaca atagaattat
      421 tttatatgaa ttttattttt gaatagttat aaataattag aaatttgtaa aaaagtataa
      481 tatattatta gtaatggtac agaatacaca tttataattt atagatgtgt attttttgtt
      541 taaaaaattt aaatatataa aactataaag gttacaattt tggaaataga tgtaaatata
      601 tgttgtattg tataataaga gtaatataaa atctagtaaa aataatcaaa taaatttagc
      661 ttatgagagg gataactatg gaaaataagg attttaaatt aacaatatgt atatttgctt
      721 ttgtaattgg agtagtagta ttattctgtt gtataggttt aggtgaattt agtgcagagc
      781 atattattca acaaaatggt ggaatcatgg atactgacca atataatatt tatctagacc
      841 aatctatttc tcaatataga aattttggtt ctatattagc tctgttagga ggaataggga
      901 tattgtttga taaatatatc agaaaatact agagattatg ttttgtctaa aataaattct
      961 tatgaggaga taaaaaatgg caacagaaat atttactgta aaaattacac ccttagaggc
     1021 acaaaaaatg ataaaagaaa atcttgatgc agatttagtt tttagtgatt cttataattt
     1081 aggagatgat aaatttatac taattacaac ttttgagaag tattatgcaa gaaataatag
     1141 tgatgcagga ttaatagtag tttatgaaaa taccacagga aaaacagtta ttaaagctac
     1201 ttcaacaggc agtgcaattg gaattttaaa aattgattgg ggtgcaggtg gaaatttact
     1261 taaaagagta aaaaacatac ttgctgatta tattatttaa ttattttatc taactgataa
     1321 tatgtcaaga aaatctcgta aaagaggtta agaacatttt tataatatat agataaataa
     1381 gaatttgaag gtaaagtttt taaggtataa agaaaaataa tataataaaa cgaattggct
     1441 ttataattct ttataaggca aagagcacat ttctttatat aattgttgga tattaaataa
     1501 tatg
//
LOCUS       DAMJWU010000229         1440 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMJWU010000229 DAMJWU010000000
VERSION     DAMJWU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.235"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            337..1326
                     /locus_tag="PC460_003720"
     CDS             337..1326
                     /locus_tag="PC460_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF2602563.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          547 a          141 c          254 g          498 t
ORIGIN      
        1 atttatctta cattaaaaaa tttaagtgga tttttcaaaa tatagataaa tttctttttc
       61 attgtttaaa tgataaaaat atatttgact tcataccaaa aagagtgccc catgaactaa
      121 aaagttaatt ttgaaatacc atattcatat atcatatgat ttatttggaa attaatatta
      181 atacattaaa ataactaaat acatgtatgc ataaagatgt ataaaaaata tataaacata
      241 tagtattaat tttaaattca acaatttaat tgttaaaagt gttattatta aattagaaat
      301 atcttaactt aatatactga gatgggagtg atagatatgt taaaagaaga aaaaacttat
      361 caagagaaag acgcaaaagt attcaatgga gtggcagctt tgataatgat tttattattt
      421 ttagtagttt caataggtat catggttttt gggatagtaa aacttgatag tggatttact
      481 ggaataggtt cacttatgtt aattttggga cttgtattta tagtagtaga ttttatccta
      541 ttttttgggc taaaaatgat taatcctaaa gaagctatag ttctagtttt gtttggtaac
      601 tattatggaa ctataaaaaa agaagggtac tattgggtga atccattttg ttcagcaata
      661 aacccagcag tatcaagaat taatgtatcg aaatcttcat ctgatgaaaa ggtagatata
      721 tcaactaatt caaggggcaa aaaagtatct ttaaaaacaa tgactcttaa taatgaaaag
      781 cagaaagtca atgatttatt gggaaatcca attataatag gagtggttgt aatttggaag
      841 gttatagatg caacaaaggc agtatttaat gtggataatt ataatacatt cttatctatt
      901 cagtgtgatt ctactattcg caatgtttca agactttatc catatgatgt atctgaagat
      961 ggagatgaaa aatctcttcg tggtagtagt caggaaatag cagatagatt aaaagatgaa
     1021 ttacaatcaa gggttgacat tgcaggaatt gaagtgtgtg aagtgagaat tactcatctt
     1081 tcttatgcac cagagattgc agcagcaatg ttacaacgtc aacaagcaga ggctataata
     1141 gcagcacgta aaaagattgt tgagggggct gtaagtatgg tagagatggc attgactaat
     1201 ttaagtgaaa ataatgttgt gactttggac gaagaacgta aagcagctat ggtaagtaat
     1261 ctgttagttg tgctttgtgg caataaagat gcacaaccag tggtaaacag cagttctatt
     1321 tattaaatta taaaaagttt aatagtttat tttattgata tatagaaaat ttagtagttc
     1381 attttattaa attatataaa gattaatggt ttgctttatt gaattataaa aagtttaata
//
LOCUS       DAMJWU010000230         1421 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMJWU010000230 DAMJWU010000000
VERSION     DAMJWU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.236"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            138..947
                     /locus_tag="PC460_003721"
     CDS             138..947
                     /locus_tag="PC460_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602564.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIEELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            989..1255
                     /locus_tag="PC460_003722"
     CDS             989..1255
                     /locus_tag="PC460_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2602565.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
BASE COUNT          563 a          160 c          196 g          502 t
ORIGIN      
        1 tgagatttta ctttacaatt attgtatcta aatttaacaa aatatcttat tagaatattt
       61 caattaaaac aaaactggtt tattatataa aaaataaatt agtatactta ttttttatat
      121 atcaggagga tatacttatg aataaaataa tcaataacat tttaaatacc tgcaaacagt
      181 atacatatat tttgccacat aaattgttaa gacagtatat agcacactat acaatatcta
      241 ttccagatac aagtattaaa gaaaatctta cattaatacc tgatgctagt ggatgtatga
      301 tatttaaatt tgacaaaaaa ggtatagaaa gcgcattctg gggagccaca acaaaaacta
      361 ctattgtaaa aaatgatata gaaaatgtac tttttagagt attcgttgag tttcgcccag
      421 gtggagtata ttatttgaca ggtctttctc aaagagaatc tacagattta aaaatttcac
      481 ttgaagattt taacacatta tttagcttag aggttaattc catttttgaa agaacaagta
      541 ctatagaaga attagtagaa caattagata tgttattctt atcatactta ttgaaatcaa
      601 atatagtaga tatgactata ccaattcttg aaaatgctag aaaacaaaac tctattatgt
      661 ctgttaaaaa catttcacaa attagttgtt atagtgaacg acatttaaat agaattttta
      721 ataattctct tggtatgagt gttaaatcat accttagatt acttcgtata aaccttgtat
      781 tacaagaaat acaaaataat aaaataccat ttgcaacttt agcacaagat attggttatt
      841 acgaccaatc tcattttata aatgatttta aatctatttg tggtgttaat ccaacaacct
      901 atataaaaaa tttgtccgat ttttacaatg aaaagtataa attttgatat acaatcatat
      961 aaaatttata tttaaggagt gacttaaaat gatagaatca agatgtggaa ttttatgtta
     1021 tgaatgtgga tacaaggaaa aagtcaattg taaaggttgt acagagattg agaaaccatt
     1081 ttggggtgaa caatgtcctg taaaatcttg ttgtgaagat aaaaacctta cacattgtgg
     1141 tctatgtgat acatttccat gtgaaatgct taatcaattt gcatatgaca aagaacaagg
     1201 tgatgatggc aaaagaattt ctcagtgcaa aaaatgggct gtactagcct tataatatac
     1261 tctcttgtta taaaataagt ttttgtaagg taaatttttt aaactgttgt agaaatttct
     1321 gcaacagttt ttataattaa atttaaaggt tatttaatta aattaataga atagtaattt
     1381 attgataaaa tctgattact aaaattggaa atataaaatc t
//
LOCUS       DAMJWU010000231         1337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMJWU010000231 DAMJWU010000000
VERSION     DAMJWU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.237"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            27..101
                     /locus_tag="PC460_003723"
     tRNA            27..101
                     /locus_tag="PC460_003723"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC460_003724"
     tRNA            116..200
                     /locus_tag="PC460_003724"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC460_003725"
     tRNA            210..293
                     /locus_tag="PC460_003725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="PC460_003726"
     tRNA            323..399
                     /locus_tag="PC460_003726"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="PC460_003727"
     tRNA            407..482
                     /locus_tag="PC460_003727"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="PC460_003728"
     tRNA            571..659
                     /locus_tag="PC460_003728"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="PC460_003729"
     tRNA            663..738
                     /locus_tag="PC460_003729"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="PC460_003730"
     tRNA            745..818
                     /locus_tag="PC460_003730"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="PC460_003731"
     tRNA            833..909
                     /locus_tag="PC460_003731"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="PC460_003732"
     tRNA            933..1009
                     /locus_tag="PC460_003732"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="PC460_003733"
     tRNA            1017..1093
                     /locus_tag="PC460_003733"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="PC460_003734"
     tRNA            1102..1177
                     /locus_tag="PC460_003734"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="PC460_003735"
     tRNA            1185..1258
                     /locus_tag="PC460_003735"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="PC460_003736"
     tRNA            1265..1336
                     /locus_tag="PC460_003736"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          320 a          309 c          355 g          353 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatatatatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAMJWU010000232         1282 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMJWU010000232 DAMJWU010000000
VERSION     DAMJWU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.238"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            436..1116
                     /locus_tag="PC460_003737"
     CDS             436..1116
                     /locus_tag="PC460_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2602566.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKVRSITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          561 a           82 c          196 g          443 t
ORIGIN      
        1 ggttaaaata attttatata tagtgcctaa tgtataacct aatgctcgta gacaataatg
       61 tggatattat caaatttatt tttataatag aaattttaat ttatatctat attaaattgt
      121 taaagatata attatgagaa taaaaaatat agccttgaat tatataattg tatatgctta
      181 tctttataca gtaaataaaa aatcaaatga aataccaaat aaagtgtata tcttaaaaca
      241 atttactaga gatacaagga tgctatgtga attttgaagt gtgcagttta gataataaat
      301 atatataaat aataaagaaa atggatttat agtatttaaa atttcttata agggtaaaaa
      361 atgctattag aaaatgtact aaaaatattt ttccaattct ttatagaaaa ataatgatat
      421 ttaggagaga ttgtaatgca aattgatgta ttgataattg atggagataa gaatagttct
      481 caagagctga aaaatttttt agaaggaaag gggataaatg tctatttagc atataattgt
      541 aaagatgcta ttataaaaat atttagcaag aaatttgact taatattttt agagataatt
      601 ttagttgatg gtgatggttg ggatttttgt aaaaaagtta gaagtataac tacttgccca
      661 attattcata taacttatat aaatgaaaat caaagtattt taaatgcttt aaattctgga
      721 ggagatgatt atttaataaa gcctttaaat ttagaaatat tatatgcgaa agtgaaagct
      781 attttaagaa gaagagattt atttttaaat aataataaaa ataatataaa actagaagat
      841 tataatagag ctagtggagt aataaagtta gaaaataaaa tgataaagtt aactccaaca
      901 gaaaatagat tattaaatta ctttatagag aatgctgaaa agactctaac tattaaggag
      961 atatatgaac atgtatggat gaatgagtat ttagatgata attatagtgt agtggttgct
     1021 gtgagtggat taagaaagaa aatagagaaa gattataaaa atcctcaaaa aattataacg
     1081 ataagagaag tagggtatta ctttaacaag atataaagct ttataaacag aatgaatgat
     1141 aaggcataga taatatgtta gtatattaca agagtttttg ttctaacgat atatttgtaa
     1201 aattagattt aatttactta attaaattat agcagtagaa taaatataaa aaatgtctga
     1261 aatattatag acatcagtaa aa
//
LOCUS       DAMJWU010000233         1273 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMJWU010000233 DAMJWU010000000
VERSION     DAMJWU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.239"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     repeat_region   51..807
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=51..79
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
BASE COUNT          487 a          179 c          118 g          489 t
ORIGIN      
        1 ttagtataaa acgtaagtaa tataaattta tgtaaagcaa caaaagatgg atttacattc
       61 catatagtta atataaaacg atttcaaatg attagaataa aaatatcaga attgctattt
      121 atattccata tagttaatat aaaactataa agtgttactt ttatttattt tattttttac
      181 ataatttaca ttccatatag ttaatataaa acgcattata tggctgtgtc attttgcatt
      241 ctattacata tttacattcc atatagttaa tataaaacct gcttaatgca gaattaagag
      301 aggaagtgtt ataagattta cattccatat agttaatata aaactggttg ttcctgctcc
      361 ggtgttggtg gaactggtac atttacattc catatagtta atataaaacg aatatgtatg
      421 gataattttt tacttaacat attagcattt acattccata tagttaatat aaaacctgct
      481 gtaatttctg atgaagcttc tccactacca ccatttacat tccatatagt taatataaaa
      541 cccttcttta actcttatcg tatttaaacc tcttataaca tttacattcc atatagttaa
      601 tataaaactt tttgtgttaa gaagaaaaaa gaaattttga taatatttac attccatata
      661 gttaatataa aaccttaaag aaaaaatgga ggaagattaa gtgaaaaaat tatttacatt
      721 ccatatagtt aatataaaac tggatttaaa taatcaaaac aactccggtt taaaaaacat
      781 ttacattcca tatagttaat ataaaacccc aaaataaact tagtattttc aacacttaca
      841 cgtatacaac tctcttaaat ttgcagtgaa cgagcaatag tgcaattgat aacgcttatc
      901 atacactctc aacgccttat atttcaactg ttacgccaaa ttttttcaca aatatcgctc
      961 actgcaaaat tcctataatt ttattatatc ataaatatat tacttttgaa tattgatacc
     1021 aatttatacc ataataaaag caaaaaaacg taatctatct acattagatt taaattctat
     1081 aacttaaagt tttttacata atatttatgc ttgtttaatc aatctaatat atcattcttg
     1141 tatattgctt ttaatagtct tttttcttaa ataaatatct atctgaacaa gcatagttta
     1201 attaaaaact tttgtattcc cttaaaactg tatctacctc ttattatttt atttatgata
     1261 tattattttt aat
//
LOCUS       DAMJWU010000234         1233 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMJWU010000234 DAMJWU010000000
VERSION     DAMJWU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.240"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="PC460_003738"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="PC460_003738"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            179..253
                     /locus_tag="PC460_003739"
     tRNA            179..253
                     /locus_tag="PC460_003739"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Asn,seq:gtt)
     gene            259..344
                     /locus_tag="PC460_003740"
     tRNA            259..344
                     /locus_tag="PC460_003740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Leu,seq:taa)
     gene            360..435
                     /locus_tag="PC460_003741"
     tRNA            360..435
                     /locus_tag="PC460_003741"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Met,seq:cat)
     gene            443..517
                     /locus_tag="PC460_003742"
     tRNA            443..517
                     /locus_tag="PC460_003742"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Glu,seq:ttc)
     gene            532..605
                     /locus_tag="PC460_003743"
     tRNA            532..605
                     /locus_tag="PC460_003743"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Gly,seq:tcc)
     gene            611..686
                     /locus_tag="PC460_003744"
     tRNA            611..686
                     /locus_tag="PC460_003744"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:644..646,aa:Val,seq:tac)
     gene            692..768
                     /locus_tag="PC460_003745"
     tRNA            692..768
                     /locus_tag="PC460_003745"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Asp,seq:gtc)
     gene            779..853
                     /locus_tag="PC460_003746"
     tRNA            779..853
                     /locus_tag="PC460_003746"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Gly,seq:gcc)
     gene            863..936
                     /locus_tag="PC460_003747"
     tRNA            863..936
                     /locus_tag="PC460_003747"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Arg,seq:tct)
BASE COUNT          353 a          259 c          288 g          333 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc atttcgaaca cagaagttaa gctccctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg ttaaatgttc
      181 caaggtagct caacggtgga gcaaccggct gttaaccggt aggctgtggg ttcgagcccc
      241 acccttggag ccaaaaaagc cgaagtggcg gaactggcag acgcacagga cttaaaatcc
      301 tgcgggactt acctctcgta ccggttcgat tccggtcttc ggcacttata aaacaatatc
      361 gcggggtgga gcagttggca gctcgtcggg ctcataaccc gaaggtcgta ggttcaagtc
      421 ctgcctccgc aaccattaaa acggctcgtt ggtcaagagg ttaagacacc gccctttcac
      481 ggcggtaaca ggggttcaat tcccctacga gtcaccaatt caattaaata tgcgggtgta
      541 gctcaatggt agagttctgg ccttccaagc cagctgtgag ggttcgatcc ccttcacccg
      601 ctccaaaaat gggaccatag ctcagctggg agagcacctg ccttacaagc agggggtcac
      661 aggttcgagc cctgttggtc ccaccattcg cggcctggta gttcagttgg ttagaatgcc
      721 agcctgtcac gctggaggtc gagggttcga gccccttcca ggtcgccaaa ttaaataagc
      781 gggaatagtt cagtggtaga gcgcaacctt gccaaggttg aagtcgcgag ttcgaatctc
      841 gtttcccgct ccataataac ttgggtgcat agctcagctg gatagagcaa cgcccttcta
      901 aggcgtgtgt ccggggttcg aatccctgtg cgctcattaa gaaaagttta ctataaatta
      961 tagtaaactt ttatattgtt taaaaagaca caggaaacat gtatattacc tgtgtctttt
     1021 tatttataaa ctttaaacta tgtaaaaatt acagtatatt ctaatagagt tacagtatat
     1081 tcattaatat tgtaaacata cggagggtaa tgtataatag tctacaagat gtttatgaag
     1141 gcatcagtta aatataataa atataactaa ttagtaagag ttatatacat tatattgtat
     1201 actttatggt atttatatat tgaaataact tat
//
LOCUS       DAMJWU010000235         1219 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMJWU010000235 DAMJWU010000000
VERSION     DAMJWU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.241"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            116..1192
                     /locus_tag="PC460_003748"
     CDS             116..1192
                     /locus_tag="PC460_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2602567.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT          475 a          173 c          216 g          355 t
ORIGIN      
        1 atataaaaaa taaattttat gttgacatag ttaattcagc ttgatattat agttattaaa
       61 aataaggaca gtaaaaacgc tatccatatt acaaactaaa aaggggggaa ttataatgag
      121 taagaaatta tttattcctg gtcctattga tgttaaagaa gaagtcttgc aaaagatggc
      181 tacaccaatg ataggacata gaggaaaaga tgcatctatg ctccaaaaga gtataagtga
      241 aaagatgcaa aaactattct atacaaacaa cacgatactt ttatccacgt catccggaac
      301 tggtttaatg gaaggctcca ttcgttcctg tacatccaaa aaggcagcag tattttcctg
      361 tggatccttt ggtgatagat ggtacaaaat ggcagtggct aataatgtcc cagcggatat
      421 atttaaagtt gaattaggag aggcaacaac gccagaaatg gttgataagg tgctttctac
      481 cggagaatac gatttaataa ctgtaactca caatgaaact tcaactggaa taagaaatcc
      541 aatagaagaa attggagaag ttgttaaaaa gtatgaagat gtaatctact gtgtagatac
      601 tgtaagttca gccggtggaa taaaagtcga ggttgataag attggaatag atatttgtat
      661 aacatcagta caaaaagcac tgggattacc accaggaatg tctatttgca cattttctca
      721 aaaagcaatc gatagagcta agcaagtacc ttttagagga gtatatcttg atttgttagc
      781 aatgtatgaa tacttaatca agaaaaacta ccaataccct tcaacaccat ctctttctca
      841 catgttcgct ttagatttcc aattagataa tatattagat gaaggcctag ataacagatt
      901 caacagacat gaagacatgg caaacttagt tagaaactgg gctaaaaaac acttccaaat
      961 ttttaccaat gaaaatcact tatccaatac acttactgtt atagaaaata ctcaaggtat
     1021 tagtgtatca aatttaaatt caaaattaca agaacgagga ttccaaatag caaatggcta
     1081 tggagattta aaagaaaaaa cttttagaat ttcacatatg ggtgactata ctgtcgaaga
     1141 tgtacaagaa ttattagata atattgatga tatattaggt tttaataaat aatattgtaa
     1201 aaatatgtat gttttaaga
//
LOCUS       DAMJWU010000236         1199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMJWU010000236 DAMJWU010000000
VERSION     DAMJWU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.242"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            67..798
                     /locus_tag="PC460_003749"
     CDS             67..798
                     /locus_tag="PC460_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602568.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(955..1161)
                     /locus_tag="PC460_003750"
     CDS             complement(955..1161)
                     /locus_tag="PC460_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602569.1"
                     /translation="MSKYSSLWEYFKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          500 a          128 c          147 g          424 t
ORIGIN      
        1 atgttatctt cataaacaaa tttatttttc acaagtacaa tacttattta aaaaaggagg
       61 tttaatatgt taagtaaaaa aaatgtaagt ttaattattt tagtaataat tttaagttta
      121 ttaactgcta tatttatttt caataataaa aatactaaca ataaattgaa agaagttact
      181 aagaataatg aagaaatagt acctaaaaac ttagaaggtt taagtataga acaattaata
      241 gagattagag ataaagccat tgataaaaat ttagaaggtc ttagggaatt tggagaagat
      301 tcagtaaaaa aacgtaaaga ttcaatttta aactttgaat ttggattagt agcttttgtt
      361 aacaagaatt atagcaactt aacagaaaaa gagaaattaa taagacaaat taatattgtt
      421 aactatgaaa actctataaa tgatataatg gatccttcag gttcattctt tgaattagca
      481 aatcagaatt accaagtgaa tgagtttcta ttaaaaaacg gaaaagatta taaagaatgg
      541 tcatatgaag gtctagattt tctaattgat catctagaag aactgtgcaa atttgttgat
      601 tttgatgtaa aagatgtaat agatattatt gatcctacaa taaaaacaga cctttcagag
      661 gaacaaaaga aatctttgaa tgataaatta aagaaaatat cttcaagtga tactttaagt
      721 gaagaagtta aaaaggagat aaaaaactgg gaaaacaatt taaataattt aaatatgaat
      781 aaaaataatt ctaattaatt ttctcataag aaaaatgctt atatcgtgat aatgtgaaat
      841 gtacataatt ataaacaaaa aaggggctgt gacactaaaa aatagtgcta cgcccttttt
      901 tatttgtaat aatcatcaat acaaattatt ttattatatg taattattta tgtcttaacc
      961 aattttatcg aaaataacag tcttctcttt taaagagatt tttcctactt gatatccata
     1021 ttcatttaat tcctttttat atttcaaaaa cgaatggtct atctctatcc cagcaatatc
     1081 ttttatttct tcaaaagtta atttaaacga ttgactattg tttttcttaa aatattccca
     1141 taaagaacta tatttactca tcttatatca ctccttttaa aatcatatct tatctatta
//
LOCUS       DAMJWU010000237         1186 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMJWU010000237 DAMJWU010000000
VERSION     DAMJWU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.243"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            207..>1186
                     /locus_tag="PC460_003751"
     CDS             207..>1186
                     /locus_tag="PC460_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2602570.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNF
                     KIGILLFFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSF
                     LQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIANLIYGIGLSIFLG
                     YVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYTP"
BASE COUNT          432 a          103 c          148 g          503 t
ORIGIN      
        1 aaaaaataat agtaaactga ttgcataaaa atgattttta tataaatttt aaacagaaga
       61 ataaatttct aacgaaattg gatatttctt ctgtaaatac atattcaaaa aaagaattat
      121 aagagtttag tcagcagtcg tgtaagtaat aaggacttta taaatatata gaaatataat
      181 gatatgagta taagtagaaa aattttatga tttttacaat tattttttat aaaaaaggga
      241 ggttatgttt gttgaattta caatcaaaat gggtaaactt tttaaagcca acatatgtca
      301 gtgatgaaat aaaaagaaaa aaactacaag ttataatatt tttttctata acttatggtt
      361 tatcttattc actaggttta ttagcaatat gttttaatca tttaatagat tcagaaattt
      421 tagctcaatt tatgatgata ttgccattat cttcagtttc tattgcaaaa ttttacacag
      481 agggaagaaa taatgataaa tataatttct attcatcagt aatattattt tttattattt
      541 acttttttat atttattatt gaatcattaa agttaataag tattaatcaa tttcaattaa
      601 tgtatcttat gttaatttta ataagtagtt tatgtgttat tgtttattct tataaaataa
      661 caagtttata tatttttaaa aattttaaga taggtatttt gctatttttt tactttttgt
      721 tttctcaaat tatttttgga ttaataataa gtgataatca atttgattat aagaatttct
      781 tgaattatat tcttcttcct ataatatcat tagtatacat ttatcctttt ttatgtgaag
      841 agtatggttg gagatctttt ttgcaaagta ttttatttga tagatttggc aaaaaaatag
      901 ggattgtaat ggtaggcact tgttggagtt tatggcactt acctttacaa tttacactat
      961 atagtcctca aactccaata attggagcta tagccaattt gatatatggt attggacttt
     1021 ctatattttt aggttatgtt tatatgaaaa ctaagaacat atggttttgc tctataatgc
     1081 atgttctaat taatagctta ggattaattt ttaatgattc tgaaataatt attaattacc
     1141 atagtattat acaaagattg atatttatac tattatttta tactcc
//
LOCUS       DAMJWU010000238         1134 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMJWU010000238 DAMJWU010000000
VERSION     DAMJWU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.244"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            23..1063
                     /locus_tag="PC460_003752"
     CDS             23..1063
                     /locus_tag="PC460_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF2602571.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          447 a          137 c          188 g          362 t
ORIGIN      
        1 tatttattta ggaggaaaca atatgaattt aaatttaact aatactataa atttaatgaa
       61 aaaatatttg gacattccaa gcccagctgg atatactgaa aatgcagttt tagaaatcaa
      121 aaaagatttt gaaaatttag gtcttgacac tattcttaca aaaaaaggtg cacttatagc
      181 tactcttcct ggtgaagatg attcaaatca aataacaata tctgcacata tggatacact
      241 tggagctata gtaaaagaaa tatcatctga tggtactctt aaataccata aagttggtgg
      301 tggatgttgg gcagctatag agggagaaaa ctgtactgtt ataactagaa aaggtaaaaa
      361 aatcagagga agtgttgtat ttaagtatgc ttctactcat atatatggtc aaacaaaagc
      421 tgccactgaa agaaatgaag aaacaatgat tattagactt gatgaaaaag tatttacaaa
      481 gcaagatgta ttagatttag gaataagtgt tggggatttt gtatgcctag accatagatt
      541 tgaggctaca gaatctggtt ttataaaatc tagatatata gataataaat cagctgtagc
      601 aatggttctt gaaatatgta gatattttaa agaaaacaat ttaacaccta agcatactac
      661 taacttttat ataagtaatt atgaagaaat tggacatggt gtttctaaat gtttacctga
      721 aaaaacaaaa gaatttgtag caatagatat agcacctact ggaccaagtc aaacatctag
      781 tgaacatggt gtaactatag ttgcaaaaga taatttatca ttatatgatt ttaatttaag
      841 aaacaagctt gttgatgttg cagaagacaa taatttagac tacgctgtcg atgtatttac
      901 tttctatggt tcagatgctt cagaagctat tcgttgggga gaagatgttc aaatagcatg
      961 tgttggtcct ggaacaaaca atagtcacca ttatgaaaga acacatatag aagctattga
     1021 aaatacacta aaattactta taaattatat gcttaaagaa taattaatta tatattttac
     1081 caaagtcaat tcttaacaaa taattaactt taatttttat ttttatacaa aaat
//
LOCUS       DAMJWU010000239         1131 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMJWU010000239 DAMJWU010000000
VERSION     DAMJWU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.245"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(206..1075)
                     /locus_tag="PC460_003753"
     CDS             complement(206..1075)
                     /locus_tag="PC460_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2602572.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          426 a          174 c          108 g          423 t
ORIGIN      
        1 atacatcaaa aaataaacta aaaaatatcg aaaaataatt taaaagacat caaaactaaa
       61 tcttctaatc agcaattaaa agtctatttt cttagtttaa aaaataaaga tagatttttt
      121 caaaacactg gataaatagg taaaaaaata aacagttagt aaaataaatt tttactaact
      181 gtttattttt attgtttaat atgtcctata aatcctctaa gtaatacttt ccttgcaaaa
      241 attctgattc taaacctaga tttttaaaat aatcgaccat gcccatacac gcaaattttt
      301 ctgtagaata atgagtagca cctataacat taattaaatt atcttttgct atttgatgga
      361 actctaatac tggttcaaaa tgttttaaag gcttagtaaa cccagtaata tatagattta
      421 taccaagttc agcaacttct cttgctacaa atggataact tcctccccct gctgcaatag
      481 caactaaacc atcttttaat acagaatctc cataatcata caatttcaca tcatgaccaa
      541 cagatttttt aattaattcc ttaatctctt ctattgattt tagtgatgtt ttacatataa
      601 ctcctacttc aattttatcg tcatatttac aaaatggttt gactatttct aaacccatag
      661 cttttgcaaa actaacagaa gttgaatagt cactatagtt gtctagtgga gaatgtaata
      721 cataaaaaga aattctacga tttttcatct cttccatata agatagtggt atgtcgtgaa
      781 atggaaaacc ttcatctgat gctatgtatc ccattgcatg atgtgaaaaa attaaaatat
      841 ctttttcatc tttatctata acttgcttaa ttatttcttt atcaggaaaa gtagttgtgt
      901 atactttatt aatattttga gcattatcta atactaatcc tatgtatttt tttctaaatt
      961 ctggagtaat aaaatcattt tcaaaattca taaaactcca atcatcatta atatttttaa
     1021 tattaaattc taaatctaat tttttataaa gttcaattgc cttaataata accactcctt
     1081 tataataata ttgaattact acatattaat atagaaattt ccgataataa a
//
LOCUS       DAMJWU010000240         1109 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMJWU010000240 DAMJWU010000000
VERSION     DAMJWU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.246"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..>1109
                     /locus_tag="PC460_003754"
     rRNA            <1..>1109
                     /locus_tag="PC460_003754"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          292 a          249 c          332 g          236 t
ORIGIN      
        1 gtagcgaagt agctgatttc acactgtcaa gaaaagtctc tatcgaggtt aaaggtaccc
       61 gtaccgcaaa ccgacacagg tgggtgagga gagtatccta aggccagcga gagaactgtt
      121 gttaaggaac tcggcaaaat gaccccgtaa cttagggata aggggtgcca ccatcaggtg
      181 gccgcagaga ataggcccaa gcgactgttt accaaaaaca taggtttctg ctaagtcgca
      241 agacgatgta taggagctga cgcctgcccg gtgctggaag gttaagggga tctgttagag
      301 caatcgaagc agtgaactta agccccagta aacggcggcc gtaactataa cggtcctaag
      361 gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaac gatttgggca
      421 ctgtctcaac aacagactcg gtgaaattgt aattccggtg aagatgccgg atacctgcga
      481 caggacggaa agaccccatg gagctttact gtagcttgac attgggtctt ggtactacat
      541 gtacaggata ggtgggaggc tttgaaacca ggacgccagt tttggcggag ccatccttgg
      601 gataccaccc ttgtagtact gggactctaa ccataggcca tgaatctggt cttgggacac
      661 tgtcaggtgg gcagtttgac tggggcggtc gcctcccaaa aggtaacgga ggcgctcaaa
      721 ggttctctca gtacggtcgg aaatcgtacg tagagtgtaa aggcaaaaga gagcttgatt
      781 gcaagacata caggtcgagc aaggatgaaa atcggactta gtgatccggt ggttctgcgt
      841 ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt atctccccca
      901 agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcatcaca tcctggggct
      961 gtagtaggtc ccaagggttg ggctgttcgc ccattaaagt ggtacgcgag ctgggttcag
     1021 aacgtcgtga gacagttcgg tccctatccg tcgcaggcgt aggaaatttg agaagacctg
     1081 tccttagtac gagaggaccg ggatggacg
//
LOCUS       DAMJWU010000241         1098 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMJWU010000241 DAMJWU010000000
VERSION     DAMJWU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.247"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            307..774
                     /locus_tag="PC460_003755"
     CDS             307..774
                     /locus_tag="PC460_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2602573.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDYFDTFVERLSTMRDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          419 a           73 c          181 g          425 t
ORIGIN      
        1 ttaatattat tagtgtatat taatattctc ttgaaattaa aaaaacatag atatttcagt
       61 aatctaaaat agtctacaaa aataacagtt ttagcaaaat taataagaat acatatagat
      121 ttaaacctaa aagtatgaat gtgtctgttt tttacaagat taaaatttat attatatgat
      181 acaattaact taactatttc aataagactt ataatttttt tctttatact agaccttatt
      241 gaaaccttta tagtttaatt ctaattatat attataaata aagtatgtgt atattaagga
      301 ggtaatatga agtttttaaa tgttttaata gtagttgaag atattgagaa atcaaaaaag
      361 ttttattatg atgttttggg gttaaaggta atctgtgatt ttggagaaaa tgttgtatta
      421 gaagggaata tatcattaca agaaaaaaag ctttggttag agtttattaa taagagtgac
      481 agtgaagtta agttcaatgg aaatgatgct gagctgtatt ttgaggaaga ttattttgat
      541 acttttgttg aaagattgag tacaatgaga gatatagatt atgttcatct agctatagag
      601 cataggtggg gtcaaagagc aattagattt tatgatttag atgggcatat tattgaggtt
      661 ggagaaacta tgtcatctgt gtgtagacgt tttttagata gtggtctttc aatagatgag
      721 gtagctaaaa gaatggatgt aactgtcgaa tatattgaat cagttttaga atagatggat
      781 tatattaaag taaaactata ttttgtatag aagtaagggc tgtaagtaaa cttgtatgga
      841 aacaaattta cttacagttt ttgtttttgt aagatacaga atataatatt tgttataatt
      901 ttatttggat ttttaaaaag tgcatgattt tccttacata ttataataga atttaatatt
      961 tattggatga tttaaattta gaaaaatata agtattagct tataaaagat aattatactt
     1021 actatcaagc tgtagaatca tatatactat tttatgtaat tatgttaaaa tttaattgaa
     1081 caaattaaaa atatagtg
//
LOCUS       DAMJWU010000242         1093 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMJWU010000242 DAMJWU010000000
VERSION     DAMJWU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.248"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            555..>1093
                     /locus_tag="PC460_003756"
     CDS             555..>1093
                     /locus_tag="PC460_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602574.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMIS"
BASE COUNT          363 a          132 c          153 g          445 t
ORIGIN      
        1 atcttttatg acttatttat atttttcatt tttataaaaa aacaataatt aagaatataa
       61 ctatattcta catgtaaaaa tttttttatt taaactcaat tttctgataa tttgataatt
      121 tttcaaaatt tatagttgac ttttattttt tactgtatta aactaattac tatacaaaac
      181 agaaaacaaa attttcaaaa ttattaaatt aaaaggagag gtaaatatga agactttatt
      241 aaacaaacaa gtacgatttt ttagtagcca atttagttag ggtttgtacc taagttaaga
      301 caaattgtca taacatagat acaagcccct taggattatg atttttaagg attgtatcta
      361 tgttggctat agtaattgtt gttaaagttt ttattttatc atcataatat agagccacat
      421 ggattatact gtgtggctta ttcgaatttt tgccatacag ttatatatac tgtgtggttt
      481 ttttatatca aaatcatttt ttaaaaccaa attttaaaaa ccatttaaaa tattttcaaa
      541 ggggtgtttt atttatgtca ggtattatat ctgtaatgac gcaaagttta attttatcga
      601 ttatggcact gggggtttat ataacttaca aaatcttaga ttttccagat atgtctgctg
      661 atggtagcta tacaatgggt gcttctattg tagcattttc tcttactaat ggtatttctc
      721 ctgtagtagc aactttaatg gctatcttat gtggttgtac tgctggtctt gttacaggga
      781 ttcttcacat taagtttaag atatctaatt tactttctgg tattttagtt atgggaatgc
      841 tatactctat caatttaaga ataatgggaa aatcaaatat tcctctattt agctttaaac
      901 acttattcaa tggggaaata tctcctatag ttttagcctt agcttttgta ttcatctgta
      961 aagtcctttt agatttattc ctaaaaacag gtcttggata cactttaaaa ggtgttggtg
     1021 ataactctca aatgattaaa tctctaggta taaatatagg ttctataaaa atactaggac
     1081 ttatgatttc aaa
//
LOCUS       DAMJWU010000243         1086 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMJWU010000243 DAMJWU010000000
VERSION     DAMJWU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.249"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(220..711)
                     /locus_tag="PC460_003757"
     CDS             complement(220..711)
                     /locus_tag="PC460_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF2602575.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          418 a          159 c           94 g          415 t
ORIGIN      
        1 aaataaaaat atctgcaact atatattgca aaaatttaaa actaatttat atatgctaat
       61 ataaatattt ataaagacat aagacaaatt tacctacaga tttaaattta acttatatat
      121 tttaaaaggc taatataata tgtattttct tctatacaat caaaaggctt catatctaag
      181 atatgtgatg acactaatat tcacaatttt tagtaattat cacattataa aaaatattac
      241 agctagtcca tcattagatg cttcttgtat aaacttttta agctcaatta gatttgccca
      301 tacatattca aaaaaatcat cttcactctc atcattcata acaggataaa tattattttc
      361 taacatctca ttaatattaa atctttttct aaatgcttgt tcttccaaac tgttcattgc
      421 ttttgatagt ttttttacat catttgctgt aatcaacatg acaggaaatt ctccatcaat
      481 ttccataagt atgtttccac ttagtactaa tttgctaaat atattatcat catctccacc
      541 aaaaggacaa cctgtcaaag taaagtgaat ggcatgccat gctttatcaa tattgatagt
      601 actattttcg ttggcatctt caaaaacaaa atcttgtaaa ttttcttctg cttgctgtaa
      661 tttaaaaaca ttctcttcac ttattttagt ataacatcct atcattccca taattacatt
      721 cctcctaagt agcgtttttt taattgtaac ttataattaa acttagttac aataaaatat
      781 attgagaaaa gtattatttc atcttaaatt taaataccta tattatcaac taaaattctg
      841 tgtttatagc gtaagtatta ttttttccta aaaattaaat gaatcagagg ttctataggc
      901 aatattgact ataaataaga actattactt tttaaaaaaa atttaagaaa ttatactgta
      961 gaatcatgct ctacttatta gctttaaata aaaaataaat catgttctca aattaaataa
     1021 taaataacaa taaatttaaa ttttaaacta taagagtttc tgaagtgtta ataagcatac
     1081 caataa
//
LOCUS       DAMJWU010000244         1080 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMJWU010000244 DAMJWU010000000
VERSION     DAMJWU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.250"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            68..143
                     /locus_tag="PC460_003758"
     tRNA            68..143
                     /locus_tag="PC460_003758"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:101..103,aa:Lys,seq:ttt)
     gene            146..234
                     /locus_tag="PC460_003759"
     tRNA            146..234
                     /locus_tag="PC460_003759"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:180..182,aa:Ser,seq:tga)
     gene            252..342
                     /locus_tag="PC460_003760"
     tRNA            252..342
                     /locus_tag="PC460_003760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:286..288,aa:Ser,seq:gct)
     gene            351..427
                     /locus_tag="PC460_003761"
     tRNA            351..427
                     /locus_tag="PC460_003761"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:385..387,aa:Pro,seq:tgg)
     gene            513..588
                     /locus_tag="PC460_003762"
     tRNA            513..588
                     /locus_tag="PC460_003762"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:546..548,aa:Trp,seq:cca)
     gene            649..725
                     /locus_tag="PC460_003763"
     tRNA            649..725
                     /locus_tag="PC460_003763"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:683..685,aa:Pro,seq:tgg)
     gene            732..808
                     /locus_tag="PC460_003764"
     tRNA            732..808
                     /locus_tag="PC460_003764"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:766..768,aa:Ile,seq:gat)
     gene            812..887
                     /locus_tag="PC460_003765"
     tRNA            812..887
                     /locus_tag="PC460_003765"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Phe,seq:gaa)
     gene            895..971
                     /locus_tag="PC460_003766"
     tRNA            895..971
                     /locus_tag="PC460_003766"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Met,seq:cat)
BASE COUNT          281 a          240 c          291 g          268 t
ORIGIN      
        1 aaggcacatg actttgactc atgtatgcgt aggttcgagt cctgcatccc cagccaaata
       61 aaaatatggt ctattagctc agtcggtaga gcacctgact tttaatcagg gtgtcccgcg
      121 ttcgagtcgc ggatagatca ccaatggaga ggtgtccgag tggtttaagg agctggtctt
      181 gaaaaccagt gacttcgaaa ggggccgtgg gttcgaatcc caccctctcc gccaaaaaaa
      241 tgactgaatt tggagaagta ctcaagtggc tcaagaggat cccctgctaa gggattaggc
      301 tgggtaaccg gtgcgagggt tcgaatccct ccttctccgc cattcaaaaa cgaggtgtag
      361 cgcagtttgg tagcgcacat ggtttgggac catggggccg ggggttcgag tcccttcacc
      421 tcgaccaact taagctttta gttcagttgg ttagagtgcc caactcatgg ctagtaattc
      481 tagggtttga gtccatgaag gttattcttc ataggggtgt agctcagttg gtagagcgtc
      541 ggtctccaaa accgtgcgcc gggggttcga gtccctccac ccctgccaaa taaaaaggac
      601 ttgaaaaaag ttggatttta gagtagtata agatagtaaa taagatttcg aggtgtagcg
      661 cagtttggta gcgcacatgg tttgggacca tggggccggg ggttcgagtc ccttcacctc
      721 gaccaagaaa agggcctata gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc
      781 gctggttcga gtccagctag gcccaccatt tgcccagata gctcagtcgg tagagcaggg
      841 gactgaaaat ccccgtgtcg gtggttcgat tccgcctctg ggcaccagga atacggcggt
      901 atagcttagt tggctagagc gttcggttca tacccgaaag gtcataggtt cgactcctat
      961 taccgctacc atctaactat atcaataaag aactttgaaa attaaacagt aggttaattt
     1021 ataaacaaga aacaaaccat aaagccagat attttgataa caatagtatc tgagcctgat
//
LOCUS       DAMJWU010000245         1064 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMJWU010000245 DAMJWU010000000
VERSION     DAMJWU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.251"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            130..882
                     /gene="cwlD"
                     /locus_tag="PC460_003767"
     CDS             130..882
                     /gene="cwlD"
                     /locus_tag="PC460_003767"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF2602576.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          472 a          109 c          163 g          320 t
ORIGIN      
        1 tattgaatgt tagataaaca tagtgaatta aataggggca gaaatataat atcattacat
       61 aataatatat taataccaat aaataaagat atatcaatat taaaaaaatt taaatgtttg
      121 tactaatata tggaatatta tagtgttttt ataagtatac atatacaggg tgatgttgtg
      181 agaaagtaca taaaacatat aatttttagt tttgcaatga tatgtctagt agtagtatcc
      241 atatttgaaa ttaaaaatat ttctgaagat gttatcaagt atatgccagt aacaaacaaa
      301 actataattt tagatgctgg tcatggaggc attgatccag gtgcattaaa taaggataag
      361 agtacatctg aaaaggatat taacctagca ataacactta agcttagaga gcttatagaa
      421 tcaagtggtg gccttgtaat attaactcga gaagatgaca gtagccttta taaagaagaa
      481 aataataaaa ctacaagaca aaaatataat gagaatttga aaaatagaaa agaaataata
      541 tctaattcta atgccaatat gtttgtctct atacatctta atgcatttga acaatcaaaa
      601 tactatggag ctcaaacttt ttatcctaaa gacaaacaag acagtaaaga attatcgaag
      661 tgtattcaag aagaacttaa aagagtagta gataagacaa acaacagaga agtcaaacca
      721 agggatgata tatatctttt gaaagaaaat aatattccat cagtactgat agaatgtggt
      781 tttttatcaa atgaaaaaga gtgtaaactc ttaactgatg aaacatatca agaaaaaata
      841 gcatgggcaa tctacatagg aatacaaaaa tatttaagtt gaaataacta taaaaatagg
      901 tctaaatatg taaatagctt gtattaaagc agttaaaatg cattaatata ggctattttt
      961 attttgacaa aaaaatattt aaaataaaag ttaaaaagtt gttgacttag aataatatag
     1021 atgatattat atatgagtgc ccaaaaggag caccaaaata agac
//
LOCUS       DAMJWU010000246         1052 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMJWU010000246 DAMJWU010000000
VERSION     DAMJWU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.252"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            137..277
                     /locus_tag="PC460_003768"
     CDS             137..277
                     /locus_tag="PC460_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602577.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(274..645)
                     /locus_tag="PC460_003769"
     CDS             complement(274..645)
                     /locus_tag="PC460_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602578.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          409 a          111 c          125 g          407 t
ORIGIN      
        1 ataattatct agggaaattt tggataatca aaatatattt tataaaatca aaatacaatt
       61 acataaactt tattaaaaat ttgttgggaa tttctgacta acattaagaa cgtaagtttg
      121 ttgggggatg gtatatgtgg aaaagtcaaa cactactgtt acaaaaaaag aaaaaattga
      181 agatatagga atgaaattag aacatttatt gagtgaagat attacttttt atgaattatt
      241 acttgagatt ttgtataaag aaaaagaaga gaattaattc tcttctttaa ggaatctttt
      301 taaataggca ttatcaatca ttttttctat agcttctcta tcttcttttt caagagaata
      361 tattttttct aagagctttt ttgttttttc acttactgat aagttctcta tgattaattt
      421 atcttctttg cttatttttg tattatcatt tacgcctaat aaataatctg ttgatgtttc
      481 taatgctttt gctaatctaa ctattatttc acttttaggt tctcttaaat tattttcata
      541 acgtgacaag ctagcctcag ttataccgac tttagtagca agttcttttt gatttatatc
      601 taattctttt cgtagatttg ctattctatc tcctaaactt tccatatgta catcactcct
      661 taccgaaatt ataagttatt ttgataaaaa gtaaaataaa attaccaaaa atataaaaaa
      721 aaaaggttaa aataataaaa tatattgaaa attaccattt gtatatgtat aatatgaatt
      781 atacaaatgg taatttgatg agaggtggta tgttgaaata agaaactgaa ttaattaata
      841 aatacataga aagttttgaa aacctttaat tgaaatataa acaatatgta caaaattttt
      901 tgcaagtaaa attaccaaat gtataaaata aatcagtagc atagataatt ttatgcttta
      961 atacattaga ttatttaatt tagttataat ggtcaaaaat gcaatctaca aataaaacaa
     1021 agtattatca aaaaagtatt ctttaagaaa at
//
LOCUS       DAMJWU010000247          997 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMJWU010000247 DAMJWU010000000
VERSION     DAMJWU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.253"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            65..820
                     /locus_tag="PC460_003770"
     CDS             65..820
                     /locus_tag="PC460_003770"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2602579.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHDTLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
BASE COUNT          384 a          113 c          163 g          337 t
ORIGIN      
        1 tattagtgat aaaataaact tatagaaata taacaaattt ttattaattt cataggagga
       61 atttatgttg aaagttataa ttagtggcta taatggaaca atgggaagag ttttagcaga
      121 atgtgtaaaa aaagataatg aattagagtt ggtttgtggt gtagctaggt ctgctgatga
      181 aaaaggtcat catagtgata caaagcttta caaaacaatg actgatgtaa ctgaaaaaac
      241 tgatgtaatt atagactttt cacatcatga tactttagag ccaatattgt catatgctct
      301 aaaaaataat acacctgtag tattagctac tactggatat gacgaaaatg acttaaataa
      361 aatagaagaa gcatcaaaaa acattcctat attattatca tcaaatacat ctcttggagt
      421 aaatatatta ataaaattag ttaaggatgc ttcaaagatg ttagaaggtt ttgatattga
      481 aatcatagaa aaacatcaca atagaaaaga agacgcccct agtggaactg ctactatgat
      541 agctaatgca attagagaag tcttgcctga aagtgaaaat acacatggta gatatggtag
      601 agattgtaaa agaaaaccaa atgaaattgg aatacactct ataagaggtg gcaatataat
      661 cagcgaccat gatgttgtat ttgcaggtga taatgaagtt cttacattaa gccatcattc
      721 tcaatctgat gaagtatttg caaacggctc tatagttgca gctaaatttt taattggtaa
      781 gcaatctggg ctttataaaa tgtcagatat attaggataa gttttgtaca taataaatct
      841 caatctattt aggtattcat attcatcaat ttgaatatct aattgattga gatttctatt
      901 ctaaaagttt ataaatttta tgcttacctt ttaaatataa tcatttttct taaatattat
      961 cttatttatt ggtatatgaa atcatattgt ttattat
//
LOCUS       DAMJWU010000248          875 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMJWU010000248 DAMJWU010000000
VERSION     DAMJWU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.254"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            22..95
                     /locus_tag="PC460_003771"
     tRNA            22..95
                     /locus_tag="PC460_003771"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54..56,aa:Gly,seq:tcc)
     gene            101..176
                     /locus_tag="PC460_003772"
     tRNA            101..176
                     /locus_tag="PC460_003772"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Val,seq:tac)
     gene            182..258
                     /locus_tag="PC460_003773"
     tRNA            182..258
                     /locus_tag="PC460_003773"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:216..218,aa:Asp,seq:gtc)
     gene            268..342
                     /locus_tag="PC460_003774"
     tRNA            268..342
                     /locus_tag="PC460_003774"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            357..441
                     /locus_tag="PC460_003775"
     tRNA            357..441
                     /locus_tag="PC460_003775"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:391..393,aa:Tyr,seq:gta)
     gene            451..534
                     /locus_tag="PC460_003776"
     tRNA            451..534
                     /locus_tag="PC460_003776"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:tag)
     gene            558..632
                     /locus_tag="PC460_003777"
     tRNA            558..632
                     /locus_tag="PC460_003777"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:590..592,aa:Gly,seq:gcc)
     gene            657..733
                     /locus_tag="PC460_003778"
     tRNA            657..733
                     /locus_tag="PC460_003778"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:Arg,seq:tct)
     gene            743..818
                     /locus_tag="PC460_003779"
     tRNA            743..818
                     /locus_tag="PC460_003779"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:776..778,aa:Gln,seq:ttg)
BASE COUNT          205 a          213 c          232 g          225 t
ORIGIN      
        1 tacgagtcac caattaaata tgcgggtgta gctcaatggt agagttctgg ccttccaagc
       61 cagctgtgag ggttcgatcc ccttcacccg ctccaaaaat gggaccatag ctcagctggg
      121 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      181 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      241 gccccttcca ggtcgccaaa ataaaatgct ggtgtggctc aacggtagag cagctgactt
      301 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catataaaat taatacggag
      361 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      421 cgaatccacc atcctccacc aaaaaaatgc gcggatgtgg cggaattggc agacgcacta
      481 gacttaggat ctagcgccat tggcgtgggg gttcgactcc cttcatccgc accattttta
      541 tttaatttaa taaacgagcg ggaatagttc agtggtagag cgcaaccttg ccaaggttga
      601 agtcgcgagt tcgaatctcg tttcccgctc caaattttat gtttatgttg taatgtgggt
      661 gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc
      721 tgtgcgctca ccacatatat attggggatt agccaagtcg gtaaggcaca tgactttgac
      781 tcatgtatgc gtaggttcga gtcctgcatc cccagccaaa cagtatggtc tattagctca
      841 gtcggtagag cacctgactt ttaatcaggg tgtcc
//
LOCUS       DAMJWU010000249          814 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMJWU010000249 DAMJWU010000000
VERSION     DAMJWU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.255"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            188..694
                     /locus_tag="PC460_003780"
     CDS             188..694
                     /locus_tag="PC460_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF2602580.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          331 a           80 c          132 g          271 t
ORIGIN      
        1 atatatttta tctatatttt acacttataa aatatgaaaa ctacttattg cgaaagtaat
       61 tgatttgttg tattataatt tacatgaatt atgtaggaca atcaaaagtc agtattataa
      121 gagtacatat attcaaattt ttaacaatta atttatataa ttaaaaaatg aagggtagga
      181 tgaagtcatg ggttttagag aagtaaaaat tgaggaatta cagtttaatc catttactaa
      241 gattggcaaa gaatggttac ttataacagc tggtgattca gaaaagttta acacaatgac
      301 tgctagctgg ggtggtgttg gagtctattg gggtaaaaat gttgttacaa cttacataag
      361 accacagaga tatacaaaag aatttgtaga tagtaatgat acatttacaa ttgccttctt
      421 tgatgaaacg tatagagaag ctttaaatat ttgtggcact atttctggta gagatataaa
      481 taaaatagaa aaagcaggac ttacaccata ttttgtggat gatacggtag cttttgaaga
      541 agctaatatg attatagtat gtaaaaagct atatcatggt aatatgccac cagaaaactt
      601 tgatgctaaa gaaaatgata aaaaatggta tccagagaaa gactatcata ctatgtacat
      661 ttcagaaatt ataaaagttc ttgtaaaaga ataattataa taaaaaatat atgaaaagaa
      721 cttattataa gtaaatgact gtgctttctt atctgatatc taagtagatg ctactaacta
      781 aataaatatt aattttaaat aaatactatt ataa
//
LOCUS       DAMJWU010000250          767 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMJWU010000250 DAMJWU010000000
VERSION     DAMJWU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.256"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(56..673)
                     /locus_tag="PC460_003781"
     CDS             complement(56..673)
                     /locus_tag="PC460_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2602581.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVGNKEIKILHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
BASE COUNT          234 a          144 c           72 g          317 t
ORIGIN      
        1 cctacatctg taattatagt gtaataactt ttatctatag ccaatatatc acctcctaaa
       61 atattgttat ctcttggtat ccaactccat tgccagttaa tacatcaatt tctccataag
      121 tttctatatc tggtggactc caagggtata tagttatttc ttgacccatt agggttgtta
      181 taccaaaatt catataattg tctttgctta taagcactct agtgtaatct aaagtcatat
      241 tacatggctt aatattactt acaaaagaat gaacttcctc aaaccaatct tgatttctag
      301 catcactttc aagatgtata ttataagtag cattattaat agttaactca taattacctt
      361 ctccaactat actatctagc caattcctta aaaatctctc tgagtaaggt aatttactta
      421 tatatttact aaaaatccta aaccttctat cttctaaact ctcattactt ttaggagtta
      481 tagacattat cttttcccat ctttttatac cacttatagt taggtcctct aaaaactggt
      541 catttgatag gtcctttaat ttatcatgta atatttttat ttctttattt cctacattaa
      601 atacttttat atattcttct ttatcttgta gaatttgtgg taagtaattt attagattaa
      661 tctctttatc caactacctc acctctcact actatactgt tactatttat tgttagatta
      721 gatttaacct catttatcat tgtatttgca atgtctaata ctccatc
//
LOCUS       DAMJWU010000251          767 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMJWU010000251 DAMJWU010000000
VERSION     DAMJWU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.257"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            95..712
                     /locus_tag="PC460_003782"
     CDS             95..712
                     /locus_tag="PC460_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2602582.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
BASE COUNT          315 a           79 c          143 g          230 t
ORIGIN      
        1 gatggagtgt tagatattac aaacacaatg ataaatgatg ttaaatctaa tctaacaata
       61 gatagtaaca gtatagtagt gagaggtgag gtagttggat aaagagatta atctaataaa
      121 ttacttacca caaattctac aagataaaga agaatatata aaagtattta atgtagaaaa
      181 caaagaaata aaaacactac atgaaaaatt aaatgaccta tcaagtgacc agtttttaga
      241 ggatttaact ccaagtggta taaaaagatg ggaaaagata atgtctataa ctcctaaaag
      301 taatgagagt ttagaagata gaaggtttag gatttttagt aaatatataa gtaaactacc
      361 ttactcagag agatttttaa ggaactggct agatagtata gttggagaag gcaattatga
      421 attaactatt aataatgcta cttataatat acatcttgaa agtgatgcta gaaatcaaga
      481 ttggtttgag gaggttcatt cttttgtaag tagtattaaa ccttgcaaca tgactttaga
      541 ttacactaga gtgcttgtaa gtaaagacaa ttatatgaat tttggtataa caaccctaat
      601 aggtcaagaa ataactatat acccttggag tccaccagat atagaaactt atggagaaat
      661 tgatgtatta actggcaatg gagttggata ccaagagata acaatatttt aggaggtgat
      721 atattggcta tagataaaag ttattacact ataattacag atgtagg
//
LOCUS       DAMJWU010000252          736 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMJWU010000252 DAMJWU010000000
VERSION     DAMJWU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.258"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..624
                     /locus_tag="PC460_003783"
     CDS             <1..624
                     /locus_tag="PC460_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDF2602583.1"
                     /translation="AYYRRMQMRKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFTTE
                     FEYFDNMRLSQDFGFNHANVVLRIGFKDGELYICNEIYVHEMDTSEIIKIANSIGLEK
                     TLFMYCDSAEPDRIKMWKNAGYKAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKE
                     IQQWKWKQDERTGLYLDEPVEFMDDAMAALRYSIDNKLKNNGISFLK"
BASE COUNT          305 a           83 c          147 g          201 t
ORIGIN      
        1 gcttactaca gaagaatgca aatgagaaaa gagcaagacc cagaagggta caaagtctat
       61 ggtcttggag aatggggaga aactggtgga gcaatactta aaaattatgt tatacatgaa
      121 tttactacag aatttgagta ttttgacaat atgaggttat cacaagactt tggatttaac
      181 catgcaaatg tagtacttag aattggcttt aaggatggag agttatatat atgtaacgaa
      241 atatatgtac atgaaatgga tacctcagaa atcataaaga ttgcaaatag tataggttta
      301 gaaaagactc tatttatgta ctgtgatagt gctgaaccag atagaattaa gatgtggaag
      361 aatgcaggat ataaagctaa aggagttaaa aaagggccag gaagtgttaa agctcaaata
      421 gattatttga aacaattaag aatacatgta catcctagtt gcactaatac cataaaagaa
      481 atacaacaat ggaaatggaa acaagacgaa agaactggat tatatcttga tgaaccagtt
      541 gagtttatgg atgatgcaat ggctgctctt agatattcta tagataataa gcttaaaaat
      601 aatggaataa gcttcttaaa gtaaaggagg tgttaaatat ttatataagt gaaacagatt
      661 taataaaagt tcagttaaaa aaagagagca cctttaacct agtaaaagtc atagaacact
      721 acatcttaaa acatag
//
LOCUS       DAMJWU010000253          736 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMJWU010000253 DAMJWU010000000
VERSION     DAMJWU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.259"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..624
                     /locus_tag="PC460_003784"
     CDS             <1..624
                     /locus_tag="PC460_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDF2602584.1"
                     /translation="AYYRRMQMRKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTE
                     SEYFDNMRLSQDFGFNHANVVLRIGFKDGELYICNEIYAHEMDTSEIIKIANSIGLEK
                     TLFMYCDSAEPDRIKMWKSAGYKAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKE
                     IQQWKWKQDERTGLYLDEPVEFMDDAMAALRYSIDNKLKNNGISFLK"
BASE COUNT          304 a           85 c          149 g          198 t
ORIGIN      
        1 gcttactaca gaagaatgca aatgagaaaa gagcaagacc cagaagggta caaagtctat
       61 ggtcttggag aatggggaga aactggtgga gcaatactta aaaattatgt tatacatgaa
      121 tttcctacag aatctgagta ttttgacaat atgaggttat cacaagactt tggatttaac
      181 catgcaaatg tagtacttag aattggcttt aaggatggag agttatatat atgtaacgaa
      241 atatatgcac atgaaatgga tacctcagaa atcataaaga ttgcaaatag tataggttta
      301 gaaaagactc tatttatgta ctgtgatagt gctgaaccag atagaattaa gatgtggaag
      361 agtgcaggat ataaagctaa aggagttaaa aaaggaccag gaagtgttaa agctcaaata
      421 gattatttga aacaattaag aatacatgta catcctagtt gcactaatac cataaaagaa
      481 atacaacaat ggaaatggaa acaagatgaa agaactggat tatatcttga tgaaccagtt
      541 gagtttatgg atgatgcaat ggctgcgctt agatattcta tagataataa gcttaaaaat
      601 aatggaataa gcttcttaaa gtaaaggagg tgttaaatat ttatataagt gaaacagatt
      661 taataaaagt tcagttaaaa aaagagagca ccattaacct agtaaaagtc atagaacact
      721 acatcttaaa gcatag
//
LOCUS       DAMJWU010000254          719 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMJWU010000254 DAMJWU010000000
VERSION     DAMJWU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.260"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(27..>719)
                     /locus_tag="PC460_003785"
     CDS             complement(27..>719)
                     /locus_tag="PC460_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2602585.1"
                     /translation="EGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLLKKYPNNV
                     DIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKPLKY
                     SLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGIYTF
                     KSSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIKLLR
                     EKLNSCLVDVII"
BASE COUNT          250 a          103 c           52 g          314 t
ORIGIN      
        1 atagaatata aaggcaaaaa tttacctcaa attattacat ctaccaaaca actatttaac
       61 ttttctctca ataattttat cttataacga cttacaatta ttgaattttg atttataaat
      121 acaatattat tttctatatc tagcatattt accttcatta aatttataag gtagctttta
      181 tgacatctaa aaaaattatg ttcttctaga tttgattcta tttctttcat actactctta
      241 aaagtatata ttcctttttc agtatgtata gttaaatcat tttttaaaac ttctaaatat
      301 aaaatatcat ctacctttat gaaatatatc ttgcatttac attttataga taaaatagct
      361 tcatttttgt agcattcttt taaatatgat gatattgctt ctttaaatag agagtatttt
      421 aagggcttct ctaaatatct ataaactcct atctcataac ctttctttat gtatttattt
      481 gatgaggata caaatataat tcttacactt tcatcaaatt ttttgatttc ttttgaaaca
      541 ttaaatccat tttcatcatt taagtctata tccataaata tgatatcaac attattagga
      601 tactttttta ataaatcgcc tccattttca aactctttga tattgtattc aaaattgtaa
      661 ttataaaaaa aggtatttat ataatcattt agaattaccc tctctgattt tttaccttc
//
LOCUS       DAMJWU010000255          710 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMJWU010000255 DAMJWU010000000
VERSION     DAMJWU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.261"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(209..688)
                     /locus_tag="PC460_003786"
     CDS             complement(209..688)
                     /locus_tag="PC460_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2602586.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          283 a          110 c           54 g          263 t
ORIGIN      
        1 ataaaaaagt acttttataa aaaaatgtgc ttttaatatc tactaataaa gttctttatt
       61 ttaattaaaa cataattata ttttagtatt ataatctttt aattttagca ttatagttta
      121 tgtattatac cctaatatat tttattttaa attactagtt agtcactctt ttcgggttat
      181 ttatctccta tatattaaag tgtcacacct aaatagttaa atcattatcc tcttttatct
      241 ttattgcctt tacaaatcca tctgcaatta caaaactcat cagagaagtt ataaaatata
      301 caaatactgg aacagtaaaa taaaatccaa atcctaaatc tagaaatctc attccttttc
      361 cagtagtacc aaaagttgaa ataaactcaa ttagagagtt taaaagcaaa agatatccaa
      421 taatcctaaa tctttttaca ttctctttta caaataggct atattcagaa ctacctaata
      481 cttctatcaa tgattttata atgataaaat atgatacacc taaaataaac attaatattc
      541 cataaatagt accttgtaga gttgcttctc cactaaaaca gaggtacata aaacttttaa
      601 atcctcctac aacaattact aatcctaaaa gaaaaaataa aatttgtaat ataccactta
      661 gtaaattttt tataagttta ttagacatca tgccaactcc tttaaaatat
//
LOCUS       DAMJWU010000256          708 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMJWU010000256 DAMJWU010000000
VERSION     DAMJWU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.262"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            163..270
                     /locus_tag="PC460_003787"
     CDS             163..270
                     /locus_tag="PC460_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2602587.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            272..>708
                     /locus_tag="PC460_003788"
     CDS             272..>708
                     /locus_tag="PC460_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2602588.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKTGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQG"
BASE COUNT          307 a           70 c          109 g          222 t
ORIGIN      
        1 ttacggttct tatatatgtt atttaaaaat aaatataaaa aaacttcaaa atacttgttt
       61 taagtaattg tatattttaa tattatgata ggttatgaaa aatggaagtt actcatggta
      121 gaggatatgc atatagtatg tcaaatatag acagccagta ttatggggag acatctttgg
      181 aatcctagtt atttcgttgc tactgtatca gaaaatacag aggagcagat aagaaaatat
      241 attaataatc agaaaaaaca agtaaagtga ggtggtagtg gtggaaaaag cttataagtt
      301 tagaatgtat ccaaataaaa aacaacaaga gttaattaac aaaacttttg gatgttgtag
      361 atttgtatat aataagtatc ttgctaaaag aatagatgtt tataaaaaca ataaagaaac
      421 ttttacttat aaacagtgta gttctgattt aaccaattta aaaaaagaat taaaatggct
      481 taaggaacct gacaaatttt cattacaaaa cgccttaaaa gatttagata atgcatataa
      541 aaaattcttt aaagaaaaaa caggatttcc taaatttaaa tcaaagaaaa ttaatagatt
      601 ttcatataaa actaacttta caaatggaaa cattatgtat tgtggtcaac atataaaact
      661 acctaaactt ggtatggtta agataaggga taaacaagta cctcaagg
//
LOCUS       DAMJWU010000257          692 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMJWU010000257 DAMJWU010000000
VERSION     DAMJWU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.263"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            84..200
                     /gene="rrf"
                     /locus_tag="PC460_003789"
     rRNA            84..200
                     /gene="rrf"
                     /locus_tag="PC460_003789"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..281
                     /locus_tag="PC460_003790"
     tRNA            207..281
                     /locus_tag="PC460_003790"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:239..241,aa:Asn,seq:gtt)
     gene            286..360
                     /locus_tag="PC460_003791"
     tRNA            286..360
                     /locus_tag="PC460_003791"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:319..321,aa:Glu,seq:ttc)
     gene            366..441
                     /locus_tag="PC460_003792"
     tRNA            366..441
                     /locus_tag="PC460_003792"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Val,seq:tac)
     gene            447..523
                     /locus_tag="PC460_003793"
     tRNA            447..523
                     /locus_tag="PC460_003793"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:481..483,aa:Asp,seq:gtc)
     gene            534..608
                     /locus_tag="PC460_003794"
     tRNA            534..608
                     /locus_tag="PC460_003794"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:566..568,aa:Thr,seq:tgt)
BASE COUNT          188 a          153 c          184 g          167 t
ORIGIN      
        1 taactctaaa aaaaaataaa aaaagtattg acaaaaatag taaaaaatgt taatattata
       61 cttgtcctag caacaagaaa aaatgtggtt ataatagcgg aggagataca cctgtttcca
      121 ttccgaacac agaagttaag ttctctagcg ctgatggtac ttggtgggaa actgcctggg
      181 agagtaggac atagccacgt gaatgttcca aggtagctca acggtggagc aaccggctgt
      241 taaccggtag gctgtgggtt cgagccccac ccttggagcc attatggctc gttggtcaag
      301 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      361 tttgtgggat catagctcag ctgggagagc acctgcctta caagcagggg gtcacaggtt
      421 cgagccctgt tggtcccacc atttgcggcc tggtagttca gttggttaga atgccagcct
      481 gtcacgctgg aggtcgaggg ttcgagcccc ttccaggtcg ccaaaataaa aatgctggtg
      541 tggctcaacg gtagagcagc tgacttgtaa tcagcaggtt gtaggttcga ttcctatcac
      601 cagctccatg taaaactaat acggaggatt tcccgagcgg ccaaaggggg cagactgtaa
      661 atctgttgtc atcgacttcg gtggttcgaa tc
//
LOCUS       DAMJWU010000258          680 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMJWU010000258 DAMJWU010000000
VERSION     DAMJWU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.264"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            90..>680
                     /locus_tag="PC460_003795"
     CDS             90..>680
                     /locus_tag="PC460_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2602589.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          276 a           70 c          149 g          185 t
ORIGIN      
        1 atagattaag tgaaatgcta ataaattaaa aaagtgaaat atcaaaataa aacgtcaaca
       61 aactaaaaat tatatggggg aatatcaaaa tgttaaatga aacaagaaaa aaacaattaa
      121 ctgaagtatg ccaagactta ataagaaatg ctagttattc aggtcaagaa gaaaacgtag
      181 taaaagctat agaagaaaat tttgctttac taggttttga tagttggtca agagatagat
      241 atggaaatat agtaggatgt ataaaaggaa ataaaccagg taaaaaaata ttatttgacg
      301 ggcatataga tacagttcca gttccagatg caagtaaatg gagtgttcct ccttttgaag
      361 cgagaatagt agatgggaaa atatatggta gaggaacttc tgatatgaag gggcaagtaa
      421 gtgctatgat ggctgctgtt tcatactttg cagaagatac taacaaagac tttgaaggtg
      481 aattgtatgt agcaggtgtt gttcatgaag aaatatttga aggtgtttca gctagagaga
      541 taagtaaggc tgttcaacct gattatgttg taataggtga atcttcagaa ttaaacttaa
      601 agataggtca aagaggtaga ggagaaatag tagtagaaac ttttggtaaa ccagctcact
      661 ctgctaatcc agaaaaaggt
//
LOCUS       DAMJWU010000259          670 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMJWU010000259 DAMJWU010000000
VERSION     DAMJWU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.265"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            210..>670
                     /locus_tag="PC460_003796"
     CDS             210..>670
                     /locus_tag="PC460_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2602590.1"
                     /translation="MYNVIDLFAGAGGLSLGFEMTKKFNMVAFVEKNDNAAKTYLENH
                     PSVKRYCDIKRLDFQDILNSVDKIDVVIGGPPCQGFSNANRQKRKIINGNNELVKLYV
                     DAIDKLKPNVFVMENVKTISSNKHSFYLTKKDKNHIINNLKLNIYNKDSVL"
BASE COUNT          292 a           49 c          106 g          223 t
ORIGIN      
        1 gattgataaa taaataaaaa atatattatg ttatttattt gaatagatta tccagaagac
       61 ataaataaga aaaataatgt gtaagtaaaa atattagaat ttattaaaag ctattacaag
      121 tataagaaat ttgaaatgat tattcaaaaa ttgacatgtg aaaaaaaata tgatatgctg
      181 caataaaagt taatgtgagg tatgtcagaa tgtataatgt tatagatctt tttgcaggtg
      241 caggtggttt gagcttagga tttgaaatga ctaaaaaatt taatatggtg gcttttgtag
      301 aaaaaaatga taacgcagca aaaacatacc ttgaaaatca tccgagtgtt aagcgttatt
      361 gtgatattaa aagattagat tttcaagata tattaaacag tgtcgataag atagatgttg
      421 ttataggagg accgccatgt caaggttttt ctaatgctaa tagacaaaaa agaaaaatta
      481 taaatggaaa taatgaatta gttaagctat atgttgatgc tattgataaa ttaaagccaa
      541 atgtatttgt aatggaaaat gtcaaaacta tatcttcaaa taagcattca ttttatttga
      601 ctaaaaaaga taaaaatcat ataataaata atttaaaatt aaatatttat aataaagata
      661 gtgttttata
//
LOCUS       DAMJWU010000260          661 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMJWU010000260 DAMJWU010000000
VERSION     DAMJWU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.266"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..372
                     /locus_tag="PC460_003797"
     CDS             <1..372
                     /locus_tag="PC460_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2602591.1"
                     /translation="DFLQKLSTKLIKENDIICIEDLQVKNMIKNHKLSRLISDVSWSE
                     FIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDR
                     DINASINILKEGLRLITIQNK"
BASE COUNT          274 a           75 c          102 g          210 t
ORIGIN      
        1 gattttctac aaaaactatc tactaaatta ataaaagaga atgatattat ttgcatagaa
       61 gatttacaag taaagaatat gattaaaaat cataaactat ctcgcttaat ttcagatgta
      121 tcatggtcag aatttattcg tcaattagaa tacaaggcta attggtatgg tagacaaatt
      181 gtaaaggtag gtaaattctt tgcaagttca caaatatgca ataaatgtgg gtataaaaac
      241 gaggaagtga aggacttaaa tataagagaa tggatttgtc ctagttgtaa tgaaactcac
      301 gatagagata taaatgcaag tataaatata ttaaaagaag gattaagact aataacaatt
      361 caaaataaat aacttatgta agaaccgtag gaactacggg gattgcctgt ggagaattag
      421 taagacatat tttagtatgc aaaattctat gaagcaggaa ccctgtgact ttagtcatgg
      481 gaggttcagt ttagcttaaa acttacttat ctatttcagt ttctattaga attattatta
      541 gactcattaa aataaaagca ctaatataac taataatata tgttaaattc attaactcta
      601 cataaagttt tacaatctaa atgaaataca ataatctgca taaaattcaa tagcttatat
      661 a
//
LOCUS       DAMJWU010000261          636 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMJWU010000261 DAMJWU010000000
VERSION     DAMJWU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.267"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(<1..634)
                     /locus_tag="PC460_003798"
     rRNA            complement(<1..634)
                     /locus_tag="PC460_003798"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          140 a          175 c          117 g          204 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccag
//
LOCUS       DAMJWU010000262          632 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMJWU010000262 DAMJWU010000000
VERSION     DAMJWU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.268"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            23..>632
                     /locus_tag="PC460_003799"
     CDS             23..>632
                     /locus_tag="PC460_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2602592.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISI"
BASE COUNT          219 a           57 c           84 g          272 t
ORIGIN      
        1 aatcattttt gaggatatta ttatgttaga tgcatttata acaattgttt cttctattat
       61 agaaatatgg tcatgtaaaa aagtttttga ttatactagt cagtcaaaga ccgacttaaa
      121 aaaactaaat atgtattttc tcttttctgt ttttctcatt atttcttctt tatatataaa
      181 tataggagct aatagtagaa tcttgatatg tatatttgta atgttcgttt tctataagtc
      241 taactataag gttaatatat ataaatgtct ggtaatctca tttttttatt ggttaatatt
      301 aataggtata aatggattaa gtatgttatt agtagcaaca gtaagcaatt tggataatat
      361 gaatgtttta cttagtggca gtttatatag atatgaagca atattcttgg gaaagataat
      421 gttaggtata ttactttatg tttataaggc ttcttttaaa aattttaaaa taagtaaaaa
      481 agagatatac atatgtattc ctatgatagc aaatttattg tcattttttg tactttatga
      541 ttatattatt aatcttgtaa aaatctctcc tgaaagaaga actgcttttt tagtaatagt
      601 tttactatta atattatcta acatttcaat ct
//
LOCUS       DAMJWU010000263          611 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMJWU010000263 DAMJWU010000000
VERSION     DAMJWU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.269"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..>611
                     /locus_tag="PC460_003800"
     rRNA            <1..>611
                     /locus_tag="PC460_003800"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          126 c          179 g          134 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg c
//
LOCUS       DAMJWU010000264          577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMJWU010000264 DAMJWU010000000
VERSION     DAMJWU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.270"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..>577
                     /locus_tag="PC460_003801"
     rRNA            <1..>577
                     /locus_tag="PC460_003801"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAMJWU010000265          533 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMJWU010000265 DAMJWU010000000
VERSION     DAMJWU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.271"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..>533
                     /locus_tag="PC460_003802"
     CDS             <1..>533
                     /locus_tag="PC460_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDF2602593.1"
                     /translation="RAMKGSAGSGKSVNVAQDYILKLGDKKYQGANLLVVRKSEATHK
                     YSTYAELTGAINRIYGKQADKYWKTTLNPLEIKSKVTGNSIIFRGVNDAKQREKLKSI
                     NFSKGKLTWVWCEEATELMESDIDILDDRLRGILTNPNLYYQMTFTFNPVSATHWIKR
                     KYFDYKNDDIFTHHSTYL"
BASE COUNT          214 a           69 c           96 g          154 t
ORIGIN      
        1 agagcaatga aaggttcagc agggagtgga aaatctgtta atgtagcaca agactatata
       61 ctaaagttag gagataagaa gtatcaagga gctaatctat tagtagttag aaagtcagaa
      121 gctacacata agtattcaac gtatgcagag cttacaggag ctataaatcg tatttatggt
      181 aaacaagctg ataagtattg gaaaactact ttaaatcctt tagaaattaa gagtaaagtt
      241 actggtaact ctataatttt cagaggagtt aatgatgcaa aacaaagaga aaaattaaaa
      301 tcaattaact tctcgaaagg aaaattaaca tgggtttggt gtgaagaagc tacagaactt
      361 atggaaagtg acatagacat actagatgac cgtttaagag gtattttaac taatcctaac
      421 ctatactatc aaatgacatt tacatttaat ccagtctcag ctactcattg gataaaaaga
      481 aagtattttg actataaaaa tgatgatata tttactcatc atagtactta tct
//
LOCUS       DAMJWU010000266          479 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMJWU010000266 DAMJWU010000000
VERSION     DAMJWU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.272"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..>479
                     /gene="tuf"
                     /locus_tag="PC460_003803"
     CDS             <1..>479
                     /gene="tuf"
                     /locus_tag="PC460_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2602594.1"
                     /translation="ATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVE
                     MEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTD
                     KPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRK"
BASE COUNT          197 a           60 c          116 g          106 t
ORIGIN      
        1 gcaacagatg gaccaatgcc acaaacaaga gagcatatac tattatcaag acaagttgga
       61 gtaccatata tagtagtatt cttaaacaaa tgtgacatgg tagatgatga agagttatta
      121 gagttagtag agatggaagt aagagattta ttaacagaat atgatttccc aggagatgac
      181 actccaatag taagaggttc agcattaatg gcattagaag atccaaagag tgagtgggga
      241 gataagatag tagaattatt cgagcaaata gatgagtata taccagctcc agagagagat
      301 acagataaac cattcttaat gccagtagag gacgtattct caatcacagg aagaggaaca
      361 gttgcaacag gaagagtgga aagaggagta ctaaaagtac aagacgaagt agaattagta
      421 ggattaacag aagcaccaag aaaagtagta gtaacaggag tagagatgtt cagaaaatt
//
LOCUS       DAMJWU010000267          440 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMJWU010000267 DAMJWU010000000
VERSION     DAMJWU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.273"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(<1..>440)
                     /locus_tag="PC460_003804"
     CDS             complement(<1..>440)
                     /locus_tag="PC460_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2602595.1"
                     /translation="EEYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVD
                     QKVGYSVGNPIVFNADDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRK
                     GEFKYVIIPAEEAIPIWDSKRQRELVAFIRFYYIEDIDGNKIKRV"
BASE COUNT          135 a           84 c           45 g          176 t
ORIGIN      
        1 actcttttta ttttatttcc atctatatct tcaatataat aaaacctaat aaatgcaact
       61 aattccctct gtcttttact atcccaaata ggaattgctt cttcagctgg aattattaca
      121 tatttaaact caccttttct attaatatat ggatgtaacc attcaacccc tttattacta
      181 gcattgagat atagttctgt tattgtatcg tcaaactctt ctcctagtaa gtcatttaaa
      241 agcttagtga gattatcatc atctgcatta aatactatgg gatttccgac actatagcct
      301 accttttggt caactaaaag cttatggtag ttgttaattg ctttattatt aactttagta
      361 aaatcatcaa ccttagctcc atctaagaga taatatcttc tcttattgtt tacatcagta
      421 ttaccataat agtattcttc
//
LOCUS       DAMJWU010000268          433 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMJWU010000268 DAMJWU010000000
VERSION     DAMJWU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.274"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="PC460_003805"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="PC460_003805"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="PC460_003806"
     tRNA            310..398
                     /locus_tag="PC460_003806"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
BASE COUNT          143 a           72 c           93 g          125 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgga tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt aga
//
LOCUS       DAMJWU010000269          381 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMJWU010000269 DAMJWU010000000
VERSION     DAMJWU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.275"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(<1..>381)
                     /locus_tag="PC460_003807"
     rRNA            complement(<1..>381)
                     /locus_tag="PC460_003807"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           89 a          107 c           87 g           98 t
ORIGIN      
        1 tgtactaccc attgtagcac gtgtgtagcc ctaagcataa ggggcatgat gatttgacgt
       61 catccccacc ttcctccagg ttatccctgg cagtctctct agagtgccca acttaatgat
      121 ggcaactaaa gacaagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga
      181 gctgacgaca accatgcacc acctgtcacc aatgtccccg aagggaactc tccgattaag
      241 gagatgtcat tgggatgtca agcttaggta aggttcttcg cgttgcttcg aattaaacca
      301 catgctccgc tacttgtgcg ggtccccgtc aattcctttg agtttcactc ttgcgagcgt
      361 acttcccagg cggagtactt a
//
LOCUS       DAMJWU010000270          364 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMJWU010000270 DAMJWU010000000
VERSION     DAMJWU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.276"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="PC460_003808"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="PC460_003808"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="PC460_003809"
     tRNA            291..362
                     /locus_tag="PC460_003809"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAMJWU010000271          362 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMJWU010000271 DAMJWU010000000
VERSION     DAMJWU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.277"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..>362
                     /locus_tag="PC460_003810"
     CDS             <1..>362
                     /locus_tag="PC460_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2602596.1"
                     /translation="IQDVGGVKVYPVWDGGGTVKLVIINSNFKVPSEDLVNLVQEEID
                     PIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWENIKSIAEEAIDDYLN
                     ELNMSWEDEENLIVRISQ"
BASE COUNT          141 a           46 c           78 g           97 t
ORIGIN      
        1 atacaagatg ttggaggagt taaggtttat cctgtgtggg acggtggagg aactgttaag
       61 ttagtaataa ttaactctaa tttcaaagta ccatcagagg atttagttaa tttagtgcaa
      121 gaagaaattg acccaattgg acatcaagga caaggcttag gattagcacc aatagggcat
      181 aaagttactg ttacaggtgt tgtaagtaca actataaata tatcagcaga gataacatac
      241 aaaaatggct acacttggga gaatataaaa tcaattgcag aagaagcaat agacgactat
      301 ttaaatgaac ttaacatgag ttgggaagat gaagaaaact taatagtccg tatatctcaa
      361 at
//
LOCUS       DAMJWU010000272          336 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMJWU010000272 DAMJWU010000000
VERSION     DAMJWU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.278"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
BASE COUNT          147 a           31 c           35 g          123 t
ORIGIN      
        1 ttgtcattaa atatgccaat agttttatat attattgatt ttattaaaaa aagagatata
       61 caaactaata agttgtaatc tctcaaatat aaaaaaacaa taaattagct cattggattt
      121 tttagtccat aatataatta tagattattc accaaacaat ctatagaaat tgtggtatta
      181 atgtttaaat aaccaacttt ttctaaaata acctttatag gaatatatga gttcataagt
      241 aaagatagcg aaataagaaa ccagtttggt atgtaaaatc tagattttta attaaattat
      301 caagattata tattatagta tcaaaaatag aaaatt
//
LOCUS       DAMJWU010000273          322 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMJWU010000273 DAMJWU010000000
VERSION     DAMJWU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.279"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            90..>322
                     /locus_tag="PC460_003811"
     CDS             90..>322
                     /locus_tag="PC460_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2602597.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKV"
BASE COUNT          132 a           45 c           60 g           85 t
ORIGIN      
        1 ccagggcaag agcctaagat aaatgatttt ggttttaatt ttactggggt aagacctcat
       61 gaaaataata ataaatagga ggaaataaaa tggcagcact aaattatgca aaagaatatt
      121 caaatgtttt agcacaagca tatccttata ctttaaactt cggggatttg tatgcaacac
      181 caaataatgg aagatataga tggactggtt ctaaaacaat agaaatacca actatatcta
      241 caactggaag agtagattca aacagagata caatagcagt agctcaaaga aactatgata
      301 atgcttggga acctaaggta tt
//
LOCUS       DAMJWU010000274          311 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMJWU010000274 DAMJWU010000000
VERSION     DAMJWU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.280"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
BASE COUNT          118 a           31 c           27 g          135 t
ORIGIN      
        1 taaaattaat atggataaat attctaaatc caatacttat taaaaatgaa ataaaaagaa
       61 aaaaagtaca aattattata tttttcttta taaactttgg tttaacttat tcattaggtt
      121 tattattgta ttataataaa tttattgatc cagaaaactt tgcatcattc atgatgatat
      181 tacctctatc ttcagttgct attgcaaaat tttatactga aggattaaat aatgatatgt
      241 atgaatttta ttcactaatc atactatttt ttcttactta ttcgttatta attgttctta
      301 agttattaaa t
//
LOCUS       DAMJWU010000275          304 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMJWU010000275 DAMJWU010000000
VERSION     DAMJWU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.281"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            <1..>304
                     /locus_tag="PC460_003812"
     CDS             <1..>304
                     /locus_tag="PC460_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HDF2602598.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPELNAKVSYAVEKADCYTGNTIESER"
BASE COUNT          119 a           44 c           55 g           86 t
ORIGIN      
        1 ctaatgtaat ccaaaagata caacaattag tacctccaac tcatccagtt ttaggtgatg
       61 gaatattagt tttaacagat ataaaatctt ctccatatcc aggagcatca gtagtgccag
      121 attattgcaa agctactttt gatagaagat tattagttgg agaaacaaga gaaggcgtat
      181 tagctccaat acaagcttta ttagatgaaa tgatgaaaga agacccagaa ttaaatgcaa
      241 aagtttctta tgcagttgaa aaagctgatt gctatacagg aaatactata gaaagtgaaa
      301 gatt
//
LOCUS       DAMJWU010000276          267 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMJWU010000276 DAMJWU010000000
VERSION     DAMJWU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.282"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
BASE COUNT           83 a           39 c           19 g          126 t
ORIGIN      
        1 ttcttttaat tctttaggat ggatttaaat tttctataaa aatcatacta ttttagctgt
       61 ttacttgctt atttaatagt tttatttctt tgattaaata tctttcagaa tctaagccag
      121 atgtctcaaa aaaactgatt tataatataa tcattttttc tgctattctt atttttccaa
      181 ttttagtttc atattaaaat atacttttca tagacactac attctttcat actcattttc
      241 acatttaact atacaagtag tcttact
//
LOCUS       DAMJWU010000277          254 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMJWU010000277 DAMJWU010000000
VERSION     DAMJWU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.denovo.283"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            68..244
                     /locus_tag="PC460_003813"
     CDS             68..244
                     /locus_tag="PC460_003813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602599.1"
                     /translation="MNLKKKEKIVKPVLKKLTEIVLNILSKKILNYAIYAISVLIVTK
                     VVINIDLYISILYY"
BASE COUNT          118 a           16 c           33 g           87 t
ORIGIN      
        1 taataaatac tgtaatataa tggaaataca gtttaataaa tataaaaaga gaaagaggga
       61 attttaaatg aatttgaaga aaaaagagaa aatagttaag cctgttttaa agaagcttac
      121 agaaatagta ttaaatatat tatcaaaaaa aatattaaac tatgctatat atgcaatttc
      181 tgttttgata gtaacaaaag ttgttataaa tatagatttg tacatatcca tattgtatta
      241 ttagatatct tata
//
LOCUS       DAMJWU010000278        20089 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.guided.065, whole genome shotgun sequence.
ACCESSION   DAMJWU010000278 DAMJWU010000000
VERSION     DAMJWU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.guided.065"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            278..616
                     /locus_tag="PC460_003814"
     CDS             278..616
                     /locus_tag="PC460_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602600.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            638..955
                     /locus_tag="PC460_003815"
     CDS             638..955
                     /locus_tag="PC460_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2602601.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1105..1782
                     /locus_tag="PC460_003816"
     CDS             1105..1782
                     /locus_tag="PC460_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602602.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2110..2877)
                     /locus_tag="PC460_003817"
     CDS             complement(2110..2877)
                     /locus_tag="PC460_003817"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2602603.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(3549..4364)
                     /locus_tag="PC460_003818"
     CDS             complement(3549..4364)
                     /locus_tag="PC460_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDF2602604.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSSRAEMERL"
     gene            4456..5724
                     /locus_tag="PC460_003819"
                     /pseudo
     CDS             4456..5724
                     /locus_tag="PC460_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            5823..6443
                     /locus_tag="PC460_003820"
     CDS             5823..6443
                     /locus_tag="PC460_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2602605.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            6593..6997
                     /locus_tag="PC460_003821"
                     /pseudo
     CDS             6593..6997
                     /locus_tag="PC460_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            7122..8048
                     /locus_tag="PC460_003822"
     CDS             7122..8048
                     /locus_tag="PC460_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2602606.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            8062..8511
                     /locus_tag="PC460_003823"
     CDS             8062..8511
                     /locus_tag="PC460_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2602607.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            8871..9278
                     /locus_tag="PC460_003824"
     CDS             8871..9278
                     /locus_tag="PC460_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2602608.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            9280..9510
                     /locus_tag="PC460_003825"
     CDS             9280..9510
                     /locus_tag="PC460_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2602609.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            9900..10103
                     /locus_tag="PC460_003826"
     CDS             9900..10103
                     /locus_tag="PC460_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2602610.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            10181..10534
                     /locus_tag="PC460_003827"
     CDS             10181..10534
                     /locus_tag="PC460_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2602611.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            10606..11238
                     /locus_tag="PC460_003828"
     CDS             10606..11238
                     /locus_tag="PC460_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2602612.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(11609..12478)
                     /locus_tag="PC460_003829"
     CDS             complement(11609..12478)
                     /locus_tag="PC460_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2602613.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(12575..13432)
                     /locus_tag="PC460_003830"
     CDS             complement(12575..13432)
                     /locus_tag="PC460_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2602614.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(13647..15665)
                     /locus_tag="PC460_003831"
     CDS             complement(13647..15665)
                     /locus_tag="PC460_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2602615.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(15936..16712)
                     /locus_tag="PC460_003832"
     CDS             complement(15936..16712)
                     /locus_tag="PC460_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2602616.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(16769..17740)
                     /gene="asrC"
                     /locus_tag="PC460_003833"
     CDS             complement(16769..17740)
                     /gene="asrC"
                     /locus_tag="PC460_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2602617.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(17753..18544)
                     /gene="asrB"
                     /locus_tag="PC460_003834"
     CDS             complement(17753..18544)
                     /gene="asrB"
                     /locus_tag="PC460_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2602618.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(18545..19552)
                     /gene="asrA"
                     /locus_tag="PC460_003835"
     CDS             complement(18545..19552)
                     /gene="asrA"
                     /locus_tag="PC460_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2602619.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKDVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         7001 a         3201 c         2591 g         7296 t
ORIGIN      
        1 acatatctaa tatgtaatta caataaaaaa tgctataatt aacatattat taattcgttt
       61 tattattgta gtatggttat gaaattatga aaatatttct ctaattaaaa atatgattta
      121 tccatatttg tttattgtag atacagacta tacaaaaata aatagcactt atttcagtat
      181 aaaaacatat atagtctata tttagactaa aaaagtataa atagtctata taaaacaact
      241 taaactatga atataatcta aagtggaggg ataataattg gcaaatatag gaaaaataat
      301 aggagataga ataaaagtct atagaacaaa attgggatat agtcaagagt ttctagcaga
      361 aaaggcaggt ttacatccaa catatattgg tcagattgaa agagctgaga caaatacatc
      421 tatcaatatt ataatgaaaa ttgctactgc tttagacata ccacttgaat tgttatttgc
      481 aaatattatt acaacagaag gcgttgataa ctctgtacct ttagaatgtt atgaaatgat
      541 taataaatta actgaaaaag aacaaattgc aatgctagag ttaataaagt gcatcattaa
      601 atacaaagaa atataagtga gggaaatagt aacaataatg aattatatag gtaaaagact
      661 taaagaagaa cggaaaaaag caaatttaac ttcaaaagaa tttgcaaata tggttggagt
      721 atcgccttgg tatataactc aaatcgaaag tggtaaaaag aatccaagtc ttaaaacatt
      781 cataaaattt gtaaatatat taaatatttc tgcagatgtt cttataaaag atattacttc
      841 cactggaaaa acatatttag aaaatgatat aaatgaagag ttaaaagact taaattcaag
      901 agaactaaat ctgatatcac agattataga catcataaaa aagaataaaa tataattctt
      961 tatattttat ataaaaaaaa aggttatttg aaataaataa cctttttttg tctttttgtg
     1021 ttaacattgt cttatagtgc taatgagtac tattagacca tgttctatgg ccttatttta
     1081 atataaaacg attggagata ggctatgatt gtgattgaag gtagcgataa atttaagatt
     1141 gcaaaagaat atattgatgt agaatatact ctttttagca aagtaacttt taggtatgaa
     1201 aagttgaaat ttaaagataa tgctgaattg gaaaaaatta agatgtttaa atataaaaat
     1261 ggctacatcc ctaataaatt aaacctttct tttggatatg gattctcttc ttataaaaag
     1321 caaataatta gagaaactgt agatacttta agattgacag aaattttttc aagcgagaac
     1381 atagaagata ttaaatttat aaaagatggt acaaaaaaat tagaaattag catagagaaa
     1441 gttgtgaaat ttaaacgtcg aaaaaaaaga aattatgttt gttgctattg ccctgatatg
     1501 tatagagaca taaaactcga caaagaatct atcaataaga tatacaacag aaaaataaaa
     1561 atagaaagag aagttaatat ttttgaagat gaggatgtta taataaacaa gaaagtattg
     1621 aagtttccaa agtcttggac aaagaatatg caaaaatatt ggttaagtga aaataagtat
     1681 cccatacatt ctactgtaat tgatgatgat agatataaat gttgtaatgt acaatataca
     1741 aaaaatagag tgataatatt atattacata tataaccatt aatatacata attacaaaaa
     1801 aataatgaca aatatacata atacaaatat atcttctact ttagataaaa cagttattat
     1861 gaaaacttac tatctataaa taatagaaat gctagtatac catctataat ctaatgtcta
     1921 gtaggtttta atggcatatt ttaaatatgc catagttgat ttataagatt ttctatatca
     1981 ctttcaaaat acacactaaa tagttgcttt tcagatataa ttgcccaatt taaattttac
     2041 tatctgaaaa gttttttata tttaatttat atcttcaaaa ataccgtcta atatttctat
     2101 ataattaatt taattaatac tcttatgtaa tagattaagt actgaattta actctttaaa
     2161 tgatatttgc cctggtgctg aaacactttt tgcagctcca aaagtcaatg cagaaccaaa
     2221 tatttcacca cacaatctac ttataacacc cattcctgac attgacattg ttattattgg
     2281 tctatctgca taaattttaa acatttcatt tgtagcttca agtagcacta acacatcttt
     2341 ttcattttgc ggcataacag ctattttggg taaatctgcg cccaattcct gcattctaca
     2401 taaacgagat acaatctctt ctttttttgg agttttatta aaatcatgat tagatataat
     2461 tactttcact tcttttttat gagcaaaatt aactacttca tctatgacct catctcccat
     2521 aaataactct acatctatta aatctactaa acctgtatta gaaatttctt tattcaaagt
     2581 agtataataa tctcttgata ttaatttttc tcccccttct actacacttc taaatgtgaa
     2641 taataaaggg atatcatgta tataacttct taattcatag agaacttcct taacttcttt
     2701 aatattttct acattctcaa aaaaatcaac tcgccactct ataatatcca aacatgcatc
     2761 ttttaactct ttagcctctt ttataatatc ttttttattt ttcccaataa ttggtacaca
     2821 aattttaggt ctaccttctc caatagtaat attttttact tgaacttttc ttttcatatt
     2881 ctcctccata atcagaaatt ctataggact ttaaattcta ttttctgcac aatttaatta
     2941 tacattaaaa atactataca ttataaaaat actaatttaa aaataatttt tattctaatt
     3001 cttaaaacaa tcccacatat tttagaaaag gtactctata atatacaaac acttcttttt
     3061 tacctaaatt tattttaaca tcctataatt ttcacaatat tctttaaatt ttactcatat
     3121 aaacaatatt attctggatg ctaatagaat taatctataa atcatagtat aattatgcag
     3181 aaataactcg aatttcacta cttatgaaga gattgaatat taatgaatct aaatcatttg
     3241 cattacttaa gagttttgac taaagttgaa caccatacaa aagtagctac tcaattgttt
     3301 ataccccaac caagcttaag taatgttata tagattctcg aaaaagaatt tggtatcttt
     3361 ttatttgaaa aacttatatt aaatttgatt atcttttcat aagttggtaa atgtcgatat
     3421 atcctgtatc tacaaggagt atcggcattt tctttttgga agatactttg cataagttgg
     3481 catttaaccg tataagaatg ccatcaaaag tagtaaataa gtagtaaact ctagtcatac
     3541 tactaggatt ataaccgttc catttcagct ctcgaagaac taaatgaacc atgtgcataa
     3601 tagccaagcg tcatagtaat gtttttatgc cccataatat attgcaatat attaggactc
     3661 attcctttgt tagccatatt tgtacagagt gtatgcctaa aggtatgtgg cgtaatcttt
     3721 ggtagcttgt cctcatgggt cttattatac ttcttgacta acgctcttaa catgctttga
     3781 taatgagttg cttgttttgg taatccatct tgtttaagaa ataggaagtt ctggtaacca
     3841 tcaacaatga ttggttctgc tttagcgttc ttacgccgtt ccacgatacg ttcaaatgat
     3901 tgataagctc tttcagtcat tggaatttct cgcttaccct gtgtggtctt tggaacttct
     3961 acataatagc caatcttagt atcactcaac aattgatggt ctaccataat ggctttattt
     4021 ttcatatcca attctaaagt caaaccgcat aattcagaaa tgcgtagtcc tgtttctaag
     4081 aggataacaa catcatcata atatttacaa taagtcttat cgctctccat aaaggataat
     4141 aagcccattt cctgttctgg tgttagagcg atttttggtt ctgttgtatc ttccaaaaca
     4201 tcacttaact gaaaattaaa tggattcttt ctaatacagt cgtcttgaat tgccatgaaa
     4261 aacgacgctt tcagtgagcg cttataattg tcaatggatt tataagcaca cccatcttca
     4321 ctcattcgga tagcccagct tttagcgtct gataatttaa ccatatcaat gctttttgca
     4381 cctagtgggt cattgatagt atctatattt tgatttataa tttatacatt tttttaaaaa
     4441 cagactttta actgtaaaga atctatgtat aaacttagct atgttgtccg tgtatctaca
     4501 aaggatcttg acgagctaaa aaggcgttgt gatgaagtca aagactttta cgacgactta
     4561 tcactcaagc tcgtgcgtcc ctttggggat atgttgggat tgcatggcga atttattcct
     4621 gtcagcaaac gttacatcaa tgattatatt caatatgtca cttcggattt tttggctgga
     4681 cttggttttg gagcgacaca aacccttggc gaacatgagg atatctatct tggctacaat
     4741 ctggacactg gaaagaatgt ctatctaaaa cctgcccttg ctagtcaatg ggtaaagggt
     4801 tctattacca atgtgttagc gtctgcgttt atcggttcat tgggtggtgg taaattattc
     4861 agtaacaata tgcttgttta ctatgcggta ctattcggcg gacaggcggt catagttgac
     4921 cctaaagcag aacgcggcaa atggaaagaa acgctaccag aaatagccca tgagataaac
     4981 attgtcaatt taaccagtaa tgatgaaaac aaaggattgc ttgaccctta cgtcattttg
     5041 agccgtaaaa atgattctga aagtttagcg attgatatcc taacattcct tgctggtatc
     5101 tccagtcgtg acagtgataa gttcccagtg cttagaaaag caatccgcaa tgtaacccaa
     5161 agtgaacagc gagggttatt acttgtcatt gatgaattac gaaaagaaga tacttctatc
     5221 agtaacagca tagccgacca tatcgaaagt tttgtagact atgattttgc acatctactg
     5281 tttggtgacg gaaatgtgaa gcagtctatc agcttggaga aacagctgaa tattattcaa
     5341 gttgctgacc tcgtattgcc agatgccaac actagctttg aagaatatac aacaatagaa
     5401 cttcttagtg ttgctatgtt gattgttatt agtacctttg cattagactt tattcatact
     5461 gaccgctcta gcttcaagat agttgacctt gacgaagcat ggtctttttt gcaggtggca
     5521 caaggtaaaa cgctatctat gaaactggtt agagcatcgc aagattcaca ggacaaacga
     5581 attgaacgtt tgaagaatta tctgactgaa aacctacaac agttgaatac tgaaatggtt
     5641 cgctcatata ttcctactag ttctattgtc aacagctttc tcagtttgag cttactttgc
     5701 agaaaaatga caactggaaa ataataatgg aatagtttct atgtaaatat atttttacta
     5761 tcttacctaa ctaattgaaa gatgatataa tttaatcaaa gatttggtaa ggagggatga
     5821 atatgcattt aattgatttt atgaatgaag atattaaaaa gaagcttgtt aaaaaaacgt
     5881 ataaaaagaa agagactatt ctttttgctg agcaagaaaa tgaatatgta attcttatga
     5941 ttgacggtat tgcagaagca ttcatcttaa atgtgaaagg aaacatctca actattcatc
     6001 tttatagaag tggtagtttt tttggagaaa tggaacaatt taatgatggg aaaaaacccg
     6061 ttgaaattgt cgctttcaca gattgtaatg tatataaact tcatagagat gattttttaa
     6121 actggttaag gggagatttt gaagctacaa aatttctgat tcgagaaata tctagtaaat
     6181 tagttgtaaa tgctgaatta attgaagaat tatcatcttt aagtgttaaa gaacgacttc
     6241 tgaggtgtat atccttacac tattatagaa gtgaattaaa tctgcttacc aaaaaacagc
     6301 ttgctagcga agtaaacgct ccaattagaa gcattaatcg tgctataaat gaatgtgtac
     6361 aagaacatct aattgcttat gaaaacaaaa gatttagtgt aattaatcag cgagaggtat
     6421 taaagaatct tcctacaaca taagtataat ttcaaaaaag tattgaataa taattattca
     6481 atgctttttt atttcgggtc aagtgaccta ttttttttta ttttttcatt atataatact
     6541 cctatgcata attatgttgg attgatagct aataaaagaa agtgagaggt gttatactaa
     6601 aaaagaagca tacttcccaa ctccagactt acagtggatt gaagatagat tttggatttg
     6661 ggttcattac gcttgcacta agattggacg tagagaacta tttgaaacaa ttgatttttt
     6721 atctttttta agacaatcag tacttgcccc attaatacag cttctcttag gtaaattacc
     6781 tcgtggagtt agaaaaattg aattggacgc accccaatat attgaagcat tagaaaagac
     6841 ggttgctaac catgaatata aaagctgtgt taatgcattg caggctacta tcaacctgta
     6901 tcttgagtta agagaggaat tatcacaaag tcctattgtc cgaaaggttg aagctgaaag
     6961 ggttgcatta cagtattttc aagatttaat caaataaacc caaagtaaaa aaatattgaa
     7021 tagtaaatat tcagtgtttt ttcatttttg gtcaaatgac ctagattagt tgttgtaacc
     7081 attctataat tattttgaaa gtaacagtat ggaggataac tatgagtgat attttaagta
     7141 gaattacaaa taaaattggg gataagaata tagttgataa gttatcagct ctatcaaaat
     7201 cagatttgaa ctcacttcta ttagaagtgt ttgatagaca agcaaatact ttaactgcaa
     7261 ctgatattct aaagtcatac caactaaata gatttactat ccctagcggt atagatccag
     7321 aagaaattca tgctttggag tcaaaattat tgagaaaagc attcaatatg gatatcaaga
     7381 cgattatgct ttctccctct gctccattag gtagctgtag cgtttttggc tccgtagatc
     7441 aatataatgt agtaagtgct ctacgaggga cagaaacact tgcagacccc tcaaatatgt
     7501 tggcaataat tattgctgat aaattaaaaa gaaaagaaga aaataatacc atacctatcc
     7561 acatggctac tactgctagg gtaataagag cacaaaattt tgtaggtaaa ggtttatatt
     7621 cacattttgg cttatattgt atggtttcat ctggtataga tacagggtct tacacttgtg
     7681 aaaaaatgtt gttagaaaag cacttaaact attataaaga tatattaagt gaaatagaaa
     7741 atgttcgttt ctctattatt ctacgcaaaa gaaacggata caaagataat gacggatttt
     7801 tcgacagaat ggttgaaact atcaaactta tttttcctaa tatagagtta tcaattcata
     7861 atgatgatgt tgataataat tattataaag gtatcaattt caaattatcg gtcaaacaag
     7921 gaaatgaaac cgttgaaata gttgacggtg gatttgttga ctggacttat caaatgcttg
     7981 gaaataaaaa agaacgctgt ttgataagtg gtattggctt agaaagattt cttgtagctg
     8041 tttcataaaa ggagaagaaa aatggaactt atagagtgga attatgctta tattaccgat
     8101 ttagttgagt ttgcgaatga taaagaaatt gcaaagaatc ttcgtgacaa cttcccccac
     8161 ccttataggg agcaaaacgc tagagatttt gttagttttt gtttggcaac tccagatgtt
     8221 aaacaactta gctatgctat tttttatcaa gaaagggcta ttggaagtat cagcctaaca
     8281 tttgaagaag atacctctat taataaaaat gctgaattag gttactggct tgctaggaac
     8341 tattggggca aagggatagc aacaaatgca gtaaaagaaa tatgcaaaat tgcctttgaa
     8401 aaatacaacc ttaacaaaat tcatgctaat gtatattcct acaatagagg gtcaagtaaa
     8461 gtattggaaa aatgtggttt taagattgaa gaatactgtc acaattcata ataaagaata
     8521 gaaacatgat tgattgccat gtatatagtt taattcgacc gcaatagcat ttattagaat
     8581 taacctactt tttaataaca gaataagttt ttcccctcta ttaatttctt atgcatcttt
     8641 gtatttttat gaaaagctag ccaatgcata tttccataca tcaatatggt ggtggcttac
     8701 aaccaatata aattaaatac gttttaaaga atacacattt ttgccctata ggcagagtgt
     8761 gtattttttt ctcttttttt tgaaatttat cgaagattta acgccctccc accgtgatag
     8821 gagtaagcaa ggttatttaa ctccttgcga tggaaagagg gtgaaatctt atggttgatg
     8881 aaaagacaat tgaacatcaa ttcgacagtt tctgtaaaac agttttgcgt aactatgcta
     8941 gagatattta tgacgaaaat aaacgcagaa atgattattt ggtatctctc gaatctttgt
     9001 cacttgcaga attaagtaaa ttgagtattt tagatgatta tgattctaac tacatctgta
     9061 tggtttctta cgactacaac atacgaattg aagatgtatt aatggcacaa gcaatcggaa
     9121 aacttacaaa aagaaaacaa gatataattc tactttcatt ctttctcaac atgacaaatg
     9181 ctgacatagc aacattgatg gatttagcag aaaataccat acattatcac aaaacaaatg
     9241 cactaaaaga gttgaaagaa ctcatggagg aacattgaaa tgaaacctac taaagaagta
     9301 ttacctttta caattatttt acttgctgta catggcgatg taaatgcaat aaacggaata
     9361 cttaaacatt ttgaacacta tatcattcgt ctttcacaga aaactttatt tgatgaatac
     9421 ggaaatccct atattcatgt agaagaagaa acaaagcgaa tgctaggaac aaaactaatc
     9481 actgctattt tgagatttaa tttgtcttaa tctcgccctc tctccatgta tcaagctatt
     9541 tggtacatgg agaatcacta attatttgtc tataaaagcc cataagctat tgtgattttt
     9601 tatagacaaa tcaatgccga ctgttctttg acaactgaat aaagtagata gatacgtttg
     9661 tgatacagat agccaacaaa tactatacgc catgacctta atcaagagag ctactatata
     9721 gtgatttgtg aataaccttt ggaacggttg ttttgccgta acctgtacca cagtataatg
     9781 atacacctgt atgacacggt tctgcccata agaatgggat tggttaaaat accaatggag
     9841 cttaccaaag ccatctatct acttctgaac tacgaacata gaaaggagtg atacattcta
     9901 tgaacaatac tgatgtacct atttgggaaa aatatacctt gacgattgaa gaagcgtcaa
     9961 aatactttcg tattggagag aaaaaattac gtaaacttgc tgatgaaaac cttgattctg
    10021 gctgggtcat tatgaatagc aatcgcattc aaataaagag aaaacaattt gagaaaatcg
    10081 ttgatacact tgactttatc taatgaaatt gagccttata tgttataata ttcatatgac
    10141 gtatcaagct caattcggtt attgagagga gcttcttatc atgtcaactg aaaaaagacg
    10201 agataataaa gatagaattt taagaactgg agagagccag agaaaagatg gaagatacgc
    10261 atataagtat gtatattcat ttggtaaacc acaatttgtt tactcttgga aacttgtacc
    10321 tactgataaa cccccaaagg gaaaacgtga tgatatatca ttaagggata aggaaaaagc
    10381 aatacaaaat gaccttgatg atagcattga cactattggt aagaaaatga cagtttgtca
    10441 gttatacgct aaaaagaatg gactaagaaa gaatatccga cttaacacta aaaaaggacg
    10501 ccattatctt atgaagattc ttaacaagga gtgattctgt gaagagatgt attgtagtag
    10561 tggaatagtc aaaaacataa tatatattaa aggagagatt taaatatgac tattccaagt
    10621 tcaaataagg tatatccaag aagtaatgat tatcaaactg tatatcttaa aaatgtaata
    10681 acaagggaaa atatagaagt tggagattac acaatatata atgactttta taatgaccca
    10741 agagattttg agaaaaacaa tgtattgtat cattacccta taaacaatga taaattaata
    10801 attggtaagt tttgctcaat agcatgtaat gcaaaatttc taatgactag tggaaatcac
    10861 tcaatgaaat caaaatccac atatactttt cccatctttt atgaagaatg gaaactcgat
    10921 atcaaccata taacggacgc ttgggacaat aagggtaaca ttataattgg aaatgatgtg
    10981 tggataggtt atgattctat aattatgtca ggtgtaaata ttggtgatgg tgcaattatt
    11041 ggaacaagag cagtagttac taatgacgtt ccaccgtatt ctattgttgg aggagtacct
    11101 gcaaaaatta taaaaaagag atttaataat gatacaattt caaaattatt agaaattaaa
    11161 tggtgggatt ggccatataa aaaaattcaa tcaaacatta agcatattca atcaggaaat
    11221 attgataaat tagtttaata ttttataatt tatttatatt aaactttact ttaataatca
    11281 ttttataaga gctttttcaa aatttgtata ttattttaat atatataaat ttaatacaac
    11341 attaaataaa ataaaaagta aagttactta ataaaaagtt actttacttt ttattattta
    11401 aattcctgtt tttattataa taggtaaaat tctattaaac tagttcacat tttcattatt
    11461 attgatatat tatatatgaa tataccattt tgttttttaa taaaaaaatc atgctacatc
    11521 aatagtaagc aactattaat gtagcatgat tttttgtatc taaataatct tagtatttac
    11581 ctatcaatat agattgatat atcaaaaact atagtcttct atattcttct ctttttagct
    11641 tttggttact tagatagtaa tcaatttcat caattagacg ttgtttatca tcaggtaata
    11701 caccttttaa attagcataa tttgatgcat gattacttct aaatacaatt ttttcattag
    11761 catctatatt ctttatcatc tctttaattt cttgtaacac agccttatcc tctagtattg
    11821 taaactctcc tgccttaagt aaatcgtaaa gttcagtatt aggttctacc attaaactaa
    11881 gtacacccac ataatctggt gaaattgctc ctaacatttt tcctgtatca actgcatgct
    11941 gatgtgtttt tttagttcct ccaagacctg ctattacagt ggctgataat ttaatcccac
    12001 tttttttaac ttttctacca acttttatta attgttcact actaaaacct ttcttaatat
    12061 cttctaatac ttcattgctt ccactttcaa gccctagata aatcatttta acaccaagtt
    12121 ttcttaattc tttaagttca tcatcagatt ttttctctat agatttagga gagccgtaca
    12181 tagtaattct ttcacactct ggaaaaactt cttttatata taagataata tctcttaatt
    12241 tctccattgg tattatcaat gcatctccat ctgctaagaa tattctttct acatgcttat
    12301 aatatcttct aaatatatca atttcatttt ttattacttc tagtggcttt attttaaatt
    12361 ttttagattt gtacatacta caaaacgtac atttgttatg agaacatcct agtgtgactt
    12421 gtattataag actataagcc tcacttggtg gtctgtaaag tggcatatcg tattccatat
    12481 taaaatcccc cttgtaataa aatattcatt ctattatatt attaatattt actttaaata
    12541 ataacttata aaattatatt ttttaaattt tagtttaaga tatattcttt aaaaatttaa
    12601 tgatactatt ggcagtaata ttaagttgtt tttcttgact tataatagca tcattatctc
    12661 ccttttgttt tccataatct ccaaattgtg catgatttcc tccttcaatc tcaacataag
    12721 ttgtatcatc aggaagtttt tgttttgatt ctacaagact tttaaaattg actactccat
    12781 ctttgctccc ccatatagat ataacttttt ttcctaattc tttcaactca tcacccattg
    12841 gataagaagc taaaaataca acgccttcaa ttaatttatt atcacgtgca aaatttgatg
    12901 ctgcaactcc tcctaatgaa tgacctccta ttacccaatg ttctatgttg ttgtaagaat
    12961 ccataacttt ctgtgcctta ttagttccaa gtatagctag attaagtggc atctttacga
    13021 ttacaacctc atatccactt tctgctattt ttcttgcaag tggagcataa gattctattt
    13081 ctactttagc tcctggatac aaaattaaac ctttagaaac ttttcttcct tttggtgtaa
    13141 atgaaatgtt ttcatttaca gttacttcaa ctttactatt gctgactaga gcttcttttg
    13201 ccagcttttg aggttcatat gttttattta tccatatgaa aaagccaagc aaaatcaata
    13261 caaatagtat acctattccc ttaaatatct tattctttat gttaaatttt ctattattat
    13321 ttttatcatc tttgttctca acctcatcat aaagtaaagg tctctcaagt tccatagtta
    13381 tactaaatgt atcatatttc ttattacgct ttacctttac atctttactc ataaatctaa
    13441 cccccataat atttgaatac tattatagtt tatcctaagt aaattgtttt ttcaaccaat
    13501 gaaaaaaatc aatattttaa ctattttcat ataaaattca taaatatatg ttttttgttt
    13561 tttatatatt attttctttt agtattttat aaaacaaata gccatatact ttattttaaa
    13621 tatatggcta ttctataatt taatatctaa gctgcttctt ttattgaaac tttagttata
    13681 ttacttattc tatacatttc tttgacacca tttagagttt gcagtaagtt tagacatatt
    13741 atcttgtcac ctagactttt agtatttaca atactctcta aaattacact tcttccttca
    13801 tcatacttat ctacattgta atttgaaata taattggtat ctctcaatat tttttcaaca
    13861 tcttcatctt ttattttatt tttattaaca tttatatata gattatacat attacttatt
    13921 agtaatttct gattttctaa aaaatcctct tgatgtttat ctttatatat agcatttatc
    13981 aacataagtc tatcttcaat aagtgatggt atcaataata aattttttat atagtaaaca
    14041 tcagctacat tttctataga cttattgact tcatcaatta tatttttaac tattccttta
    14101 tacatttcta taacatgttg atagcctgtt ttagcatcat atggtaatat aaacttattt
    14161 actattaatg caataattgc tcctaatgct acatatgaaa tcctgcttat tgttaaaaca
    14221 tttgggtctc ccataactac tgctgaacca agagctgaaa cagttacaca taccattaat
    14281 tttctataat caactacata agagtttata taccctgcta aaagaactat taacgacctt
    14341 aatgtactat cttttattat tgaaaataat acaataaata ttacagctcc tataaatgta
    14401 ccttctattc tttttctaga cctttgtata caattttctg cgtaaggttg tgttaatgaa
    14461 aagacagtta acattatcca tcttccatct cttaaatgga aataatccat aataaaaccc
    14521 gctacagctg tagctaaagc aatctttact gcataagaaa atcttataga atttaaatta
    14581 aagtttttct tatatattga tagtcgttta aattcatgag gtatctttga ttttctttca
    14641 gctttctttt cgtctcttga ttttactttt ttataatcta ctaaaaactc atataaattt
    14701 tctaaaacac tataaatttc ttttaaattt atatttttag tatcctgagc aataacataa
    14761 tctctaaatc ctaatatctc tttatcagtt atggtatctt tattttctaa acacatctta
    14821 atatagttta tttctttcgt tgctacaact aaaacatctg atttactatt tatgctactt
    14881 tcttctaaaa tattttttat atcttcatct tcaaattctt tactatcctt tttgtatctt
    14941 tccaggatta aacttattct ttctaatgaa aataaaatat tcattatgtc tatacctcta
    15001 atactgatat aaaagtcatc ttttctatta tcaaaaataa ccattttcaa atttttagat
    15061 atagaattta ttctataatt acagctatct atttttctca tactagattt aattatagaa
    15121 ctatcatcat taatagtatt ttttaaaagg ctaatttttt ctaataattc atcacatata
    15181 gatatcagtt ctttatctcc gacatttttc atcctatttc tattcataac aaattgagca
    15241 atcattatta taactgctcc aacagctaaa gccgacaatc tcataggaaa ttcacttttt
    15301 cctacaggta tacttaccat aaatatatac attagaccaa agggtacata tactgctctt
    15361 tttaagtcat aactaaatac ataccctatt ataaataatg ctataaaatt taataaaact
    15421 cctaaataca tattttgtac tgcaaaaaat gatagtattc ctaagattat atttattgct
    15481 aaatacttca gtaaattttt aattggagaa atagttaaat ctctttccaa gagtgttagc
    15541 atagctgtta ctatacctac acctataagt gtatttttac ttccaaataa atatataaat
    15601 atagaaataa atgctattat aaaaataaac aactttgttt ttgatattac tagtttcttt
    15661 gtcatcatat cccccctaaa aatttcaaca agaccaattt tatcatattt gttatcttat
    15721 tcaaaatgcg cttttttacg gtaggacttt aacttttttt atatttagaa ctatataatt
    15781 catatataaa cgtatttttt atatatataa ccgatatatt ttcgtatgct cccatatttt
    15841 ttttgtttca aatatttttt caaaaatata aaaataactc tctcaacaat actaaaaatg
    15901 tatttatcaa gagagttata atttatacaa taaatttaat taagcttttc tttacctaat
    15961 atataagttc caagacccat aaataaagct cctccaacaa tatttccaag tgttacagct
    16021 actaaattat aaatagctcc tccaatagta actgtactta ttgttggtga aaataatgaa
    16081 acactataaa ttgtcatatt tgctacacta tgctcaaaac ctgatgttat aaaagcaaat
    16141 aaacataaga atatcataat tatttttgca gtatcactat tagttctaaa cgaacacaac
    16201 actgatacac atactagaat attacataaa atccctctaa agaaaagagc tgtaaatggc
    16261 atggatgctt tactagctgc tgtatttgca aaaaattcag ctacaggtcc tttgtctact
    16321 agaccagttc ctacaaatat aatgcctaaa accaaagctc cgattaaatt acctacccaa
    16381 ctgtatgccc atattttaga agtatctttt atacttacac ctttatttaa cataccagca
    16441 gacataacca tattgtttcc agtaaataac tctgttcccg tcattataac caaacttagt
    16501 gctattgcaa atgataaccc cattactatc ttagtcatag gtgaacctgc atcagtcaaa
    16561 agtcctccaa tagtaaatat caaaagtata cctataccta cataaagtcc tgcaaacgca
    16621 gaagatacca aatattttac tttacttgtg tttaataaat ttatcttatt tatagctgca
    16681 ttagttaatt tatccaaagt ttctttatgc ataactccct cttccttcta ttaaatttta
    16741 atttattcaa atatatgttt tactcaattt atagatattt aattccactc cagtatatgt
    16801 tttcgtacat tttagttatt tctggaagct ctacaccttc aagtgcccat tttttaaatt
    16861 ccataaatcc tgttctatca attatatatc ctatatgttc ttttcctcct ggtgcatctt
    16921 tatctatata cttttctaca tatctataag tattaagtat tatctttatt atgctgtcct
    16981 catctaccca taaaagaaaa tcttctgcta gtctagggtt tttctttcct gttctaccca
    17041 ttatagctaa tctatagtat tttttctcat ctctagtcca tgcatttgtt gggcaattta
    17101 gtacacactc tccacagcct atacacttat ctttatctct taccacctta ttattttctg
    17161 tttttaatgc tccaacagat atcttagaac atttttttac acatgctcca caatttacac
    17221 atctatcctt ttcatatatt ggtaaagcca taccaataat tccaaagtca ttcattctag
    17281 cttttataca atcatttgga caaccagtta aagccacttt aaaatgtaaa tcatttggaa
    17341 atatagcttt ttctattctt ttagcaaatt cagttgtatt atattgagcc tttggacaga
    17401 ctttatttcc tatacaagct gctatatttc ttgtacctgc tgctggataa cccgcgtttt
    17461 tctcactttg atttatattc attttttcaa taacaggttg tataatttta tttatttctt
    17521 ccatatcttc catatttatt cctaatattt caaacccctg tcttgtagtt atgtgcacct
    17581 gtccattgcc gtactcactt gcaattttag aaactacagc taaactctca gggtcaataa
    17641 gacctccagg tactctaact cttaaagaag tctcatattt tgtttttgta attctataag
    17701 catttttcat tacttttttg gtatttaaat ctcttatcac aaacttcacc ccctaatcaa
    17761 taagattttt agcatatgaa taatcaaata ctggtccatc taaacatatg taagtatcat
    17821 ccattttaca atgtccacat ttacctattc cacaacacat ttttctttca taagaaaccc
    17881 aaatgttctt ttcatctaaa tctcttttta aaaattcttc tactgtaaat ttcatcatca
    17941 ttggaggtcc aactacaact atagaagtat tgtttatatc tttcatatcc aattctggta
    18001 tgtattttgt tactaatcct acattaccac tatatccttc ctctgctcca tctacagtaa
    18061 ctaaaatatc aagtttttta ctccatctct ttaaatcatc tgcaaataaa atatcttttg
    18121 gtgatttaaa tcctactatt aatttaaact tttcgcattt ttctttatta ttataaaaat
    18181 attcaactat tcctcttaca ggggctaatg cacttcctcc tgccactact acaatttctc
    18241 ttccttcata aagggataca tcaaatccat ttccataagg acctcttata aaaaaagaat
    18301 ctccttcttt ataattaaac aactcgctag ttacttttcc tacatttctt attgtaaagt
    18361 caatataatc ctctccatat cctgatactg atattggact ttcaccgtat ttaggtatag
    18421 atatctcata aaactttcca ggcaatacac cttttgtatt gcagtttact ctaaaagtcc
    18481 attctttatc agtatgttta actatatcta gtatttttgc acttactggt atatatgggt
    18541 tcatttatac ttcctcctta ctaattacta aatcatttat tttttctata cactttgaaa
    18601 atgatatgta ttgcggacag gcatcatcac atcttccaca gccaacacac atatgatatc
    18661 cgaatctttt tttaaaatca tgtattttat gcatagtttt aaatctcatt ctttcgcctt
    18721 gtttttttct aaaagaattt ccacctgcta tatctgtata tccatctact tgacaagaag
    18781 cccaaaccct tcttctctca cctacatttt cgttttcctt atagaaaata tcctgcattg
    18841 taaaacatga acatgttgga catacaaaat tacatctacc acatcctata catcttgtat
    18901 catattcatc ccatattgaa tttcctatca tttcatttga atcaatattc tctggcacat
    18961 ctacagatat taaattatca gttacataat caacattaaa ttcagttgct tctccattaa
    19021 atacttcaaa ttcactatcc tttatatcta agtacagtgt atctccttct atatttaatc
    19081 caattgaata attatcagtt ttgttagaat ccatgctcac acaaaaacag tttctaaaac
    19141 tttccttaca ccctacaagt gcaaacttta ctttatctct gatattttta taaaaataat
    19201 ctttttcttc accattattt agatatattt catcaagtct ttttatacca tttatgtcac
    19261 aagctcttaa gaaaattaaa attttcttgt tgtctaaatc gctttcctta aactcttttt
    19321 cagtaaaata aaacaatact tgatttatgg gaagtacaac atcttttggt gaaaagtttg
    19381 ctttcttagc aaattccatt tcttcaaaag cgtttacctc cttgtatctt ataacatcag
    19441 tatctgaaaa agtaccttta aatggtatta aaactggtgc aaatatcttg tagtcttttt
    19501 ttagataatt cagaccttca ttgaacttag atgaatctaa ttttagtttc atttttatca
    19561 cctttcttat attttagttt tacttactta ctatcttgtt taaatcttaa cactttattt
    19621 tctatgcttc cgtgataatt atcacagttt caaaaataat aaaaaaatat taatggagtt
    19681 tattaagttc tgttaatatt tttttattat ctacattatt caatttcaaa tctaatcact
    19741 taaatacaat cattaagtac aataaaacta ctatctactc aatttttatg caaaaaacat
    19801 actaaaaagt tgattatccg ctaaaacata gtatctatca gcattttcaa ggaaacaaac
    19861 acatcaattt actactaatt tactaccaat gaatgagcct tgatacgcaa gttttcctag
    19921 atatgcaaag atatggtacg gcacatcagt attgaagcaa gaaaaaaatt gaatatttca
    19981 tagactatgg aacgcctaaa ttgacgttcc ttttctattt ccagccgttc attatggacg
    20041 gctattttat tacccaaaat gaaaggagca tttagaatta tgaaaaaga
//
LOCUS       DAMJWU010000279        17239 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.guided.078, whole genome shotgun sequence.
ACCESSION   DAMJWU010000279 DAMJWU010000000
VERSION     DAMJWU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.guided.078"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(295..819)
                     /locus_tag="PC460_003836"
     CDS             complement(295..819)
                     /locus_tag="PC460_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2602620.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(884..1636)
                     /locus_tag="PC460_003837"
     CDS             complement(884..1636)
                     /locus_tag="PC460_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2602621.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1667..2965)
                     /locus_tag="PC460_003838"
     CDS             complement(1667..2965)
                     /locus_tag="PC460_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2602622.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2982..5246)
                     /locus_tag="PC460_003839"
     CDS             complement(2982..5246)
                     /locus_tag="PC460_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2602623.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5501..6319)
                     /locus_tag="PC460_003840"
     CDS             complement(5501..6319)
                     /locus_tag="PC460_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2602624.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6312..7010)
                     /gene="cbiQ"
                     /locus_tag="PC460_003841"
     CDS             complement(6312..7010)
                     /gene="cbiQ"
                     /locus_tag="PC460_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2602625.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(7013..7291)
                     /locus_tag="PC460_003842"
     CDS             complement(7013..7291)
                     /locus_tag="PC460_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2602626.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7281..8033)
                     /locus_tag="PC460_003843"
     CDS             complement(7281..8033)
                     /locus_tag="PC460_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2602627.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8215..8407)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8539..10311)
                     /locus_tag="PC460_003844"
     CDS             complement(8539..10311)
                     /locus_tag="PC460_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2602628.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10325..11254)
                     /locus_tag="PC460_003845"
     CDS             complement(10325..11254)
                     /locus_tag="PC460_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2602629.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11483..11671
                     /locus_tag="PC460_003846"
     CDS             11483..11671
                     /locus_tag="PC460_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2602630.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11664..12365
                     /locus_tag="PC460_003847"
     CDS             11664..12365
                     /locus_tag="PC460_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2602631.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12367..13290
                     /locus_tag="PC460_003848"
     CDS             12367..13290
                     /locus_tag="PC460_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2602632.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13447..14370
                     /locus_tag="PC460_003849"
     CDS             13447..14370
                     /locus_tag="PC460_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602633.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14363..15100
                     /locus_tag="PC460_003850"
     CDS             14363..15100
                     /locus_tag="PC460_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602634.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            15111..15884
                     /locus_tag="PC460_003851"
     CDS             15111..15884
                     /locus_tag="PC460_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602635.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(16425..17033)
                     /locus_tag="PC460_003852"
     CDS             complement(16425..17033)
                     /locus_tag="PC460_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2602636.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5583 a         2975 c         2159 g         6522 t
ORIGIN      
        1 ataaataaaa taagtttaaa aaatactgaa ataagtgttt tccacatatt ttatatattt
       61 atttaataca ttattattta aaaataaata tataaacaat cataataaaa tttttttaat
      121 aaaactttat tttttagtta aaatcataca atatatagat ttccacaata caaataaatt
      181 aaaaagtgaa atgtatttgt ctaaaaacat aataacattt cactttatcc tcatttttaa
      241 gtaatatata tttttaattt ttatttataa ctaaatctag tttttatata aattttagtt
      301 tttttctaca tctgagtaat ctattccaaa agtttctaca gtaacagatt tcataatttg
      361 tggagtttgt ggcttatcag ctgaatctgt tctaactgat gctatttcat caactgtatc
      421 cataccttct atcactctac caaaacctgc atactgacca tctaagtgtg gtgagttttt
      481 atgcattatg aaaaattgag aaccagctga attaggtgcc atagttctag ccattgatat
      541 gactcctctt tcatgtttta aattgttagt aaatccatta ccactaaatt caccttttat
      601 tgagtgacct ggaccaccca ttcctgtacc atttgggcat cctccttgta tcataaatcc
      661 tggtattact ctatgaaata ttattccatt gtaatatcct tcatttacta atgatacaaa
      721 gtttttaact gtatttggag ctatatttgg atataactca attttgattt cttttccatt
      781 ttccatttct atagttacta taggattttt attttccata atttattacc ttccttttca
      841 ttagttaaat tacataatta catttattat attatactct aaattaaagt ctatcaagta
      901 tattttttcc tatttcatat ccttcttgat aaatagtttt acaaaactca tcatcaaagt
      961 taagcgtgtc tttaggagca aattgagtat taggtctaac tactattatt ttatcttcat
     1021 catatacttt tttaatatct tctggcaact cataattata ttttgtggat attaaaataa
     1081 ttttatccac attatcccca attaatggtt caattaatgc actcctaatt aaacctccat
     1141 ctaatttgaa accatcataa attccttctt gcacatcttt cacaaattgt tctttaaatc
     1201 caagagttgc gtttggattt gatggtaaca acccactgta tgaaatgctg ataatggctt
     1261 cattacgagg catttttgat acatcaacaa tcttttctga tactacttta ttatcaactt
     1321 caacataatt aacataaaag ttcatttctt ctatattact atcatctagt tcccttaggt
     1381 tgtctatagc aggagaatta tccacagtat tatttactat ttttacattt ccattctgga
     1441 tatcttttat atttgtccac attttctcca agttatcaac attccctgtg tacaaataat
     1501 atccatttat tgctcctatt gatgtccctg aaatcgaata aaatttattt attcctccat
     1561 catacaaggc tttaactact cctgcttgat aagccccttt tgctcctcca ccttctaaac
     1621 ataatcctaa tttcatagtc tcatctccaa attgtttatc ttatatttat atttcaaaaa
     1681 acttactaaa tgttttatat atactatagt ggtcctcaac atttcctaat tctcttatag
     1741 aatgcattgc taaaattgga cttcccacat ccacagatgg tatatctata tgagtagaag
     1801 atattggacc tatagtactt cctcctcttt catctgacct attaacaaat tcttgatact
     1861 caacattagc ttctctacat atatttttat aaatagctat agaatatcca tcactagtat
     1921 acgcctgact agcatttatc tttataaccg gaccttttcc cattattggt ttattagttg
     1981 ggtcatgctt ttctggaatg tttggatgaa tagcatgagc taaatctgat gatatcataa
     2041 aagatgaata aatagactca aagaatccct ctctatcctt acccattgat atacaaattc
     2101 tctccaatat atttaacaac atgtttgaat ctgcaccttg tttagtagag ctaccaactt
     2161 cttcattgtc aaatacagcc attacattta caccatgttg acctttagta ttaacaagag
     2221 cattcaaact agcatgtacc attgcaagat tatcttgtct cccaatagat ataaattcat
     2281 cattagcccc tattaaactt cctttttcaa attcatataa aaataaatca aaatctataa
     2341 tatcgtccat atttacatct aagttagatg ctatttgctt tataagaaaa ttatcttttt
     2401 ctaatgtatc atttaacatc ccaactaaag gtaacatatc tttttgctta ttatatgaat
     2461 gaccatcatt tatacttcta tttaaatgta ttgctagatt tggtattata caaattggct
     2521 ttttaaagtc tataagcctt tcatttggtt ttaatatact atcacctttt aaaactactc
     2581 taccagctat cccaagaggt ctatccaacc atgtacttaa tattgctcct ccatagcatt
     2641 ctgtatttaa cttcaaataa gatttttcag cttctatttc tgcatttggt ttaatactaa
     2701 atgtaggaga atctgaatga gagcctatta ttctaaatcc atctctttct atatcttctg
     2761 aatttactac aaaagctact atagctgata aattcttagt aacataatac tttccaccaa
     2821 ctcttagatt ccatctttga ttcaaaacta attcttcaaa tccattttta tctaagattt
     2881 ccttagtact cttgactgca tgaaaagacg acggactatc atatatgaag tctattaagt
     2941 ttttagcaaa gtttatacta tccattcctc atttacctcc attataaaat atttagtgtt
     3001 gtatattctt tatttatttt tttaattccc tgttggtcaa aagctatagt aacatctgta
     3061 ccaattatag aaaccactgt cccaacccca aatttaggat gatgtacctt agaaccaagt
     3121 tttatatcat ctggattagt ttctttatta gaatctttta ttgtagcatt aactttatct
     3181 gcaactttag atttgcttat agtactcata tactttttag tatatttatc taacatatta
     3241 taacttgctt tagaatagct tagttctttt tttactttgt acaatctttc tatacattct
     3301 tctggcagtt cttccataaa tcttgaagct attgcaacat ttgttttacc atataatgtt
     3361 ctcttctctg tcaatgacat atatagctct tctttagctc tagttatacc aacataacaa
     3421 agtcttctct cttcttctat ttgtgaatca ctcatagatt ttatagctct tgatatagga
     3481 aataaccctt cttccatacc tgttaaaaac acaacaggga actctaaacc tttagatgta
     3541 tgaattgtca taagcgaaac cctatcattg tcttcttcat catttgattc agatgtaagt
     3601 gctatacttg ttaggaatgt ctctaagtct ttattttcat aagtatcatc attactactt
     3661 tcaaactcca aagctataga aataaattct ctcaaattat caattctgtc ttgagcttct
     3721 tcacctttgc ctgttaaatc tttttcattt ttatttctta tctctacaag ctcgtccata
     3781 tatcctgtag tgtccaagac tttttctata agtttgctaa caggataaac ttcttttata
     3841 gttctaagag tccctataat atcaacaaat ccacttatac ttgcccttgc cttagtagat
     3901 atatctgaat ttgtttctat atcaattaat actgagtata tactttcctg ttttaagtta
     3961 gctctatctt caattttttc tatggttcta agccctatac ctcttctagg aacatttata
     4021 atccttttta tagatatatc atcttgtgga ttttgaataa ctcttaaata cgccaacaaa
     4081 tcctttattt cttttctctc atagaatttt gtaccaccat atatattgta tggaatttga
     4141 cttctattta aagcgtcctc tacaggtctt gcctgtgcat ttgctctata aagtactgca
     4201 aaatccttat aaggtctatt ttcttttcta gctatttgtg caattgaatc agcaatgaaa
     4261 tcagcctctt ctatttcact accagttaat tgtatcttta taagttctcc ttcttttttt
     4321 tcactccaaa gcttttttct ttttctttct atattattag atataacttt attagctgca
     4381 tctaatatca cttgtgtaga cctataattt tgttccaact ttacaacatg tacattgtca
     4441 taatctttct caaattcaag tatgtttctt atatctgcac ctctccatcc ataaatactt
     4501 tggtcatcat ctccaacaac acaaatattt tgatgttctc ttgctaataa ttttatcaat
     4561 tcatattgag ctttacttgt atcttggtac tcatctacca ttatgtatct aaatctactt
     4621 ctgtaatatg ctaacacttc atcattagcc ttgaaaagtt ctacagtttt aagtattaaa
     4681 tcatcaaagt caagtgcact atttcttttt aaacggtctt gatatagcgc ataaatatct
     4741 gcaatcttaa ccattctatt atctgccata ttgatatctt taaactgctt tggagtataa
     4801 agtttatctt ttgctcctga aattgcactt ataactgctt ttggttcaaa cactttatca
     4861 cttaaattta attcttttag acagtcttta accaatgtaa cttggtctgc tgaatcataa
     4921 attacaaaac ttctattgta accaatctta tttatatctt ttcttagtat acgaacacag
     4981 caagaatgga atgtacttat ccacatatcc ttagtttcag gacctatatt ttgttcaact
     5041 ctctctctca tttcatttgc agctttgttt gtaaatgtta tagctaatat atttggtgct
     5101 tgtacacctt tatcttctat aagatatgct attcttgttg ttaatacctt agttttacct
     5161 gaaccagcac cagccagtat aagtactgga ccttcagtct tttctactgc ttctctttga
     5221 gcaggattta atgtatctaa attcatattt ctcctcctgt taacagccat aaatatattt
     5281 taatctaaaa tatgaattac ttcaaattat aattatgtat tatattaata gattatttcc
     5341 aatatattac aattaaataa catttttcca tataatttat ttcattgttt aataaaaaaa
     5401 ataaccttat gatttgggga atcataaggc acctttaagg ggtctaggtt aagggttatt
     5461 atatataaat ctattttttt aaaataggtt tagggcttaa ttatttattt gtattaagtt
     5521 ctctttcctt ccaatactta tacaaatctt cttctttctt aaataagggt aaattcatat
     5581 ttctatgtac ttttactaac catggggatt ctaggccagc ttgtataata ttattttcat
     5641 ttataaacac attttctgct tttccttcat caatcaaaat ccccttatcc aatacatata
     5701 tgtaatcaca tatttcatac attaaattca tatcatgact ggatattact atttttatat
     5761 tatttttatt taatcccttt attatatcta caatagacct agtacttact gggtctaaac
     5821 cagctgtagg ttcatcaagt aatacaattt cattttccat tgctataact gatgctattg
     5881 ccactctttt tttctgtcca tagctaagaa aatgtactgg cctgtctata aaatctattc
     5941 cattaacagc tactagagct ttgtttattc tctctttaat tgttttttca tccataccaa
     6001 tgtttctcat tgcaaaggct atatcatcat atacttttga ataaaaaatt tgtttttctg
     6061 ggtcttggaa cacaatacct acattttttc taaggttgta taaacttctc ttatcatatt
     6121 ttaacttttc ttctttaaaa agaatttctc cagatgtagg ctttaatata cccattaaat
     6181 tcataaataa tgttgatttt cctgaaccat ttgaacctat tatacctata acatttccct
     6241 ttgaaaaatc catattaatg tttaatagtg cattggtatt tttttcatat tgatacgtta
     6301 aattatttat tttaaacatc attatcccct actatgtgaa atttaccatc atacaatttt
     6361 atgtctaatg atatacacat atcatcatac cttgtcataa gtcttttgta cagcatactt
     6421 ccaagaatac ctaaggattt atatgaagtt tttaaattta tatatccaaa tcttagctct
     6481 tgtgacttgt atatttctga aaattcttct aaaaatatga atataaacct atatattaac
     6541 attgataact cgacaaaagt gtcaggtaaa tgaagttttt taaaaaagaa tattaattga
     6601 ttaaaaggtg ttgtaagtat acaaaaatac acacaagtca aacaagacat cgctctaaat
     6661 aacacatgaa tagacatgtc aatagacgcc tttgatattc ctacattaaa actaaaaaat
     6721 tcaaaactat ataataaatc tgctttatta aaagatatat ttacaagagt tattcctata
     6781 ctaagaaata aaaagtacat tggtattcta agtaatttta aataattttt taaatctatt
     6841 ccagccacac aaacaaccaa aatactcatt aaaatcataa ttccacttaa aataaatata
     6901 ttttgtatta ccatagaagc aatcagaaaa attactccta tggctacttt tttgtttgga
     6961 tttactttac tcaatctatt tgtatatgca tatttatcaa ttataagcat gattaacttc
     7021 tctttccttt ttgaagacct aatatgtatc ctattacacc agcccctaaa gctgcttgtg
     7081 cagagaataa taaactttct atttctccac ttggtggctc atatggactg ctaaaccatg
     7141 gtttgtaatt tggatttatc ttagtaattt cactttcagc ttgaccatct gctcctccat
     7201 attcagcacc tgagttgaca agtaatggga atataataag agctactact aataaaagta
     7261 ataatatgtt agtttttgtt ttagcactca ttagctttaa ctcctttatc attattttct
     7321 agtatgcttt tttctccgtt ttctgtaatt aaattatata caattacagt aagtatacct
     7381 tctgctattg ctattggtat ttgagtcata aagaaaattc ctaaaaattt aattgctgaa
     7441 gccattaccc cacctgatgg gtctgggaaa gccagtgcta attgtattga agttattgta
     7501 tatgtggcta agtctcctat tgctgctgca agaaatactg gaatagcatt atttccatct
     7561 tttttcttta aagccttaaa tattaggaat gatacgattg gccctattat cgccattgaa
     7621 aaagcattag ctcctaaagt agttattcct ccatgtgcta ataacaatgc ttggaatatt
     7681 aaaactatag ttccaagaac aaacattacg cttggtccaa acattattgc tccaagacct
     7741 acaccagtag gatgtgaaca acttcctgtt actgaaggta tttttagtgc tgataataca
     7801 aacacaaatg ctccacataa agctaataat actttctttt tagggtcttg ctttactatt
     7861 ttacctattg attttaatcc gaataagaaa aatggtataa atataactcc ccatgctata
     7921 gaccatttta ctggtaagta accttccatt atatgcattg catttgatgt aactggagtt
     7981 aaaatgatta acataagtgc agctataacc cctaatttaa tattctgttt catcttagta
     8041 tccctctctc cctttctaat aattaaaaca taataataca tgtctgattt atttaataaa
     8101 ataaaaaagc ctaactcgat ggttaggctt ctaattttcc acaatctaaa tacaaacaca
     8161 taaaacaaac atatctgtat aattattata agaaactttc tccatccccg aagaaattac
     8221 tttaatattt taggcaggtc tcctgactta gcttcatcct actcctcttc cttcccatga
     8281 gatataaaaa atatcatcat agtggctaac aaaagtttcg tctgcatcac agtagcgggg
     8341 gctgtaaagg aatttaacct ttttccctat taatcttgct taaagaacct ataatattat
     8401 atatttaatt tttaaattct tcttaaatta ttttctatat aaatcattgt ataccttttc
     8461 tgtgaaaatt tctacatttt ttataaattt tattcatttt tatattttta tgtatataaa
     8521 ttcttttttg taagtttatt aatttctaat tatcttcttt aaatacattg aaactacatc
     8581 agaaaatcta gttatatctc ttgaatatat aaaatcattt ccatatatta atctttcagc
     8641 ttcaagttct gattcttttc ctgtaaatac tcctaatact aatatatttt gctttctagc
     8701 ttttctcact tcattggctg tatctctcaa accaactata cccctataag ccttttctcc
     8761 tcttaatgtt ctctcactac tttttcctat ctttacatca tttggtcttc catcactgag
     8821 aactatcagg attttatttt cttcatctct atttattaga ccactacata tagattttat
     8881 tgccaaacca tctctattat taccagcaca aaaatattca aatatatttt cactattttt
     8941 tatactatca tcataatctt taaatctttt taatattgta tagtctaaaa aactagaaaa
     9001 tcccatcact ctatttggta tgccaacttc tgttagagca tttgcaatta tgtaagcttg
     9061 aattgccaca tttccctgat ttctactttg agagccactt gcatccaata atatatcaat
     9121 aacgtatttc cctttttcat tttctatatc tttataaaat attttattat tattacttct
     9181 accaactctc caagctctat tggctaatat agtaccacaa tcagaatata ctctactttt
     9241 agcattttct tctattaaaa ttcttgatag actttctttt aatctcttta tttgctgtct
     9301 atacattcta gcatgttctc taaattttcc taaattattt tctttttgtc ttgttacata
     9361 ttttagttga aatacattat tacactcact tctaagaact ccatctgtaa agtgtattct
     9421 acaaccttca tgaacatttc tacaattttg tgtctcaatt tttttaattt cacctttact
     9481 caaataagtt tttccataat aatgttctat ttttgaatat atcttatttg cagtgtcttc
     9541 atcaacatat ataactcggt ctgctgaatt gctacattta gaatcatcaa aatcccctac
     9601 accttctaca agcatggatg aagatattga atctatatca ctttcaataa tattttcttc
     9661 ttcatcacta taaagttctt catacatgaa atcagcaaat gtatcaaaat ttacttctac
     9721 actttctaca ttattttcat caataagact attttcttct ccatttaata tgtattcaaa
     9781 ataagttaaa tatataacat ctaatgcttt taaaatctcc ataatatctt gattggtatc
     9841 tatatttctt atatcattaa ctattcttct agtcatacta tctaatgctg ggtgcttgtc
     9901 catactataa agtacaaatg tgtatttaat tttatccata atattatcat tgtgtatttt
     9961 ggtaaaacgt gaaacaatat ctttataagc atttcttctt atgtcttcta tccctggcct
    10021 atctttaatg accttttctt caactattgt atttaatata acttgaatta gacccagtat
    10081 aatatctttg ttgtatcctt ttcttattct atttgtaaga tattttttaa ctatatccca
    10141 atctacatac tttcttctag cacctgcaat tattgcaaaa tatagaccta tttcttttga
    10201 agagtaatct ttttctgata tatctatact ttcattgtaa tcaccagcta ttgtccatga
    10261 taagttatta gctctatttt caaattcata atcatcatat atccatttca taatctcacc
    10321 tatattaaat atgacttatt ggaaacacat ctatactctc ccatttatta ggtattctag
    10381 ttttaattac atctccaacc atttctttct catatacatc aaaagtcttt cctgtaagtc
    10441 ccatattaat tgcaagagtt ggttttaatc ctcttcttat agtcttaaga gatgccataa
    10501 gaccacgtaa atcaatagct ttactagata tctctccatt ttgagacttc aattgtaaat
    10561 ctaaaaatat tcctgcaaag tgtattagtt tttcttcatc agcatctgaa aattcatttt
    10621 ttaaaatcat cattaactta tcttcttcta taggaggaat atctattacc ataaatctag
    10681 ataccagtgc ttcattaagt tcttttgttc ctgcatattc ataattcata gtcccaataa
    10741 atcttgttgc tggatgtaga ttgactctct catacccagg tacatcaatt attcttctat
    10801 agtctaaagc tgaatgtacc acaactatag catcattttt tgccatatta atttcatcaa
    10861 acaccccaaa acctccatta atagcacact cataaactga gcctcttcta agtttaactt
    10921 cattgtctat aaatgtatct gtacctataa gtgttgaact gtctgtattt atatgaaatg
    10981 atgtattcca ctgaggtcta ttaaatattt cccctagatt atcagcaagt aagttttttc
    11041 ccgttgcctt tggtcccgat aataatatat tttcttcttc taaaatagct gctatacaca
    11101 tagacaagat atctttacca taaaaataag ttttagactt tgttactctg tattctaaat
    11161 ctttttctaa tccataataa tttctaaaat acagtacatc ttcaataagt ttttcatcta
    11221 ccccttgcac ccttagatta tctattatat ccatattttt acccctttta tataaatagt
    11281 atttgtttca tttcttatca ccattttagc ataaaaggat ataagctaaa attgattttt
    11341 taatctcccc cattttaagt aaaaccatgc accattttat cactacaaaa ttaagagtac
    11401 tacttactga tatttcttga ttttattcgt ctatacgtat ataatatata ctgataaatt
    11461 atgaggaaag gatgattgaa atatggaata tatgtcttgc ccagaagtag caaaaaaatg
    11521 gggtatttct gaaagaagag tacaaaaact atgtgaagaa agtcgcatac caggagtttc
    11581 aaaactcggg tatatgtggc tgataccaaa gaacgcagaa aagcctgttg atggtagaac
    11641 aaaaagaaga aaggaagtac aatatgaata gcgttttgat tatagatgat gacaaagaac
    11701 tttgtgctct tatgaaaaaa tgtgttgagc aagaaaattt atctgctatt actgcacatg
    11761 gtggtgcaga aggtctacat ttagtcgata aaaatagaca cacactctct cttgtaattt
    11821 tggacataat gatgccagat atagacggtt ttcaagttct gaaaaaaatt agagaaacaa
    11881 gtaatattcc agtaattatg cttactgcta aaaatgacga ggatgataaa gtttccggac
    11941 tacgtttagg tgctgacgat tacctaacaa aaccgttcag cataaacgaa cttatggctc
    12001 gtgtcaattc tcttattcgt cggtatacta tgctaaaccc tgcttttata acagacacag
    12061 atttcataat aataaatggc atgattattg acaagacaaa ccgaattgtt tctgtcaata
    12121 atattcctgt tcaacttaca agcaaagaat ttgatttact tgcttttctg gcttctaata
    12181 agggacgggt gtttaccaaa aaacagattt atacacaagt atgggaagaa gaatatgctt
    12241 ttgacgatag taacatcatg tctttcatca gtaaattgag aaaaaagatt gaaccagacc
    12301 ccaaccatcc attttatatt ttgacagttc gtggtgtcgg ttatcgtttt aataaggagg
    12361 cttaacatga atacaagtgt ctatctgctt ttgatattgg ctcttagcct gattattata
    12421 gcgtaccttg tttctgtcct gctacgtgtc cgaatacaac ttacacttat aaaagatgct
    12481 ttagaggata taaaaaatgg aaaccttaat cgacgtattt taacagatga aaacgacatt
    12541 acaaaacaaa tttgctatga tattaacgaa atagcaatca atagccaatt tcaacttatt
    12601 cagcaaaaac aatcagaaca agcctacaaa cagctaatga caagtctctc ccatgatgta
    12661 aaaactccac ttgcttcttt ggtcggttat ttggaagcag tagaaagtaa aattgttgta
    12721 ggagaggaaa aagacgagta tatccatgtt gcgtctaata aggctcatta cttaaagaat
    12781 tttgtagaaa atctatttga atgggtaaaa ttggattcta aggaacaagt ttttcatttt
    12841 gatatttttg acttgaatga attatcccgt aatattatat ctgattggat acctgtttta
    12901 gaaagcagtc attttgaata tgaatttgat ataccagaaa tagagtactt cttacgtata
    12961 gacgcaaatg catatacccg tatcattaat aacctattac aaaatattat tacccatagt
    13021 agtggaaaca aaatgacatt gagaattttt gaaaacaaag agcaagctca aattataata
    13081 acagataatg ggaaaggtat atcttcagac aatctcccac atatttttga aaggttgtac
    13141 caatgcgacc attcacgagc ttcaaaggga aatggtttag gcttggcgat tgcaaaggaa
    13201 cttattaacg ctcataaggg aaatattacc gccaacagca ctcccggtat gggaacagaa
    13261 tttacaatat tgcttcctaa agccctgtaa aagtacgagg ctttcttact ctaaaacaag
    13321 ataaaagcaa gattatggca aggttttggc aaggttcatg ttttataatt ctaattgtag
    13381 ttgattttga cgttgtatca actatctttt gtagaaacaa atttattaaa agtgaggaaa
    13441 ttatatatga atgattttgt gattgaaaca aagcaactta caaaaattta tgggaatcaa
    13501 acagtagttg acaaagtcaa tctccatgta aaaaaaggcc gaatatatgg tctgttagga
    13561 cgcaacggag ctgggaaaac cacaattatg aaaatgattt taggattgac ttcaatcacg
    13621 tatggcgagg tagatgtatt tggagagaat attaagggac gtgaaaaaca agtatatccc
    13681 cgtatcggtg caattatcga aacgccagga ttttatccta atctgacagg aacggagaac
    13741 ttggagattt ttgcaaaact gcgtggtaca gccgccccta attctgtaaa aagagcattg
    13801 gaaattgtag gcttacctta taaaaataaa aagctgttta gtaaatattc acttggcatg
    13861 aaacagcgac ttggtattgc caacgctatc ctgcatgacc cagaactgtt agttttagat
    13921 gagccaacca atggtcttga ccctattggt attgcggaga tgagaaagtt tattagggac
    13981 ttgagcgttg agcatggaaa gacaattctt atttccagtc atattctttc agaaatctca
    14041 ttgttggctg atgatattgg aattatcgac cacggtgttc ttttggaaga aagtagtatg
    14101 agtgaactag aaaagaaaaa cagtaaatat atcctgctac aagtttcaga tattccgaag
    14161 acatcactta ttttagagcg tcaatttaac acgaaggatt attccgttca agatgactac
    14221 acgttacacc tttataacac taatctggat atgggtgaaa tcaataaagc acttgtaatg
    14281 cagaatgtaa ctgttattag ttctcaactt tgtaatgata ctttagagga ctactttaaa
    14341 aaaatcacag gaggagaggg cattgcttaa acttattcaa acagaatttc ttaaactgcg
    14401 tcgtaggaaa cttatttggc tgatgttgtt gtctgctctc attatgccgt ttttcgcact
    14461 actatacttc aattattttg gtaaaacagg tgttgagcca ataaagtttt ataaatggtc
    14521 tgcatttggt tatacacttt ggattatttt gcctgttgtt ttgggtatat tttgcaccat
    14581 gcttatacac gaagaaaacc agtatgatat gctcaaacag ctttggattg tacctattag
    14641 taaaatggga tactttttta gtaaattttt cgttgttttg atttactcta tttgttttat
    14701 gctgattaca gccgttgctt ctgtgttatt tagtgtactt tctggatatg tcgtatttag
    14761 ctgggcaagc attttatatt tgttgaaaaa atgtttagaa attggtgtaa tcactgcttt
    14821 tgtaatgttg ccgattttgg caatcgctac atctcaaaaa ggatacattc tcccaatttg
    14881 tatcacattg gtatatgcct tttccggttt tgttataatg acaataaata tgtatttaca
    14941 cccattatcc agtatgggag ttattatcat gagaaataaa gacattccag gtcttatatt
    15001 ttcacaggaa attaatgtcc cattagcatt tctttgtatt tgtatttgga gtattgtatc
    15061 agtattgttt gcaaatattg cactaggaag aagaaagtga ggtgtagata atgtggaaat
    15121 tactttgggc tgaatgtcaa aaaattcgtc gttcaaaaat tgtttggatt acagtttttg
    15181 catccattat gatagcagtc atagtttttg caggggggca ggatgtatat aatgacccag
    15241 atttacatta tggcttaaag agcgtttatg acggttcacg gtatatagac aatgcaggtt
    15301 ggtatatgga cgcagtacaa ccttggtcaa ctttttttgt tcttcctgct gtcattgccc
    15361 tattaggaag ctatattatt tgtcgtgaag aagaggagga cactattaaa tccctgcgtc
    15421 ttattcccat aaatgaaaca aatttaacca tagcaaaaat gattattact tttatattca
    15481 gtatacttct ttatttgtta ctctttacta taacattttt gactgaatcc gttttacatt
    15541 tttccgattt atcgacaaaa ttggttttaa gttgcatgaa agagtatttt cttgatggca
    15601 taggtgtatt ctttgcaatt tctcccatta ttgccttggt atcgcgcatg aaaaaaggat
    15661 attggcttgc attagtattc acagaagttt attctattgc tggactattt gcgggtatgt
    15721 caaatgtctt acaaaccttt tttcctattg cggcaatatt taatatttct ggatactata
    15781 taactactgg agaaaaaatt atggggagta tcatgagttt attactttgt gcctgccttt
    15841 ctgcatttat acttaaaggg ctaaaacaca atgaaaaaaa ttgaaatagg tatatcaaaa
    15901 tgaaaaagat aaaaaaactg attattctaa ttgtattctt gtttcttttt ggcatgttac
    15961 actgttatac tgaatggaaa ttcaacaata acccacctaa aggggtaata gaaagaataa
    16021 atgagtggac tgataagatt ggactggatt agtaacttat gttaattata gtgaatgtaa
    16081 tattagcaag ttattgtttc gtattttaat actataactt taaatatttg gttttaatct
    16141 tccaaacgaa tataaaaaaa ctgtcacaga ggcaacatat ttttgaatat ttataaagtg
    16201 gtagttttga ttttcaaagc taccgccttt tcttctatat aagcaaactt atcctcaaca
    16261 aagtgtaaaa catggtgcat tttaagaaaa aagtaccaca gacacaagca ataaaagaaa
    16321 cgccgagaac ctacaaaaac gtagtgctta tgcagattta cgacgccttc tattttctca
    16381 accgtcttaa tgaccttgta caaaagaaat ttttctaata ttttttataa tcttatcttg
    16441 tgatgttttt tatgtataaa tgacttaata acaaatccaa taaaaattat ggatattggt
    16501 ataattagaa cgaggaattt ataatcatta taatgtgaca ttattcttac ccaactctca
    16561 gaaaaaatat atcctatact gattaatgaa gtattccata tggttattcc cacgatagat
    16621 gatattacaa aagttataat attttgacct gctatacctg ctataaaaga tatgtatgtt
    16681 ctacataaag ggattaatct acatacacaa acagaaatag aagagtattt attaaaataa
    16741 tcttgagatg caaatattcc tttctttgcc tttggacata tctctattat tttatttatg
    16801 atagatttac ctccccaatt tcccactgta taacaaaata tcgaaccaag tatacctgca
    16861 attatactta aaattaaaat tgtcagaaat ccaaaatgat ttcttgcagc aattgctcca
    16921 cataaaggta aaacaacttc gctaggtaat ggaaaacaag catactcaat cataattaat
    16981 acaaatacag agattaatcc atattgtaaa ataaaactat tgattacaga cacttactta
    17041 tctatctcct ttcttattta aaggttttaa gggtacatgt tctaaggatt ctcaccctat
    17101 atttaaagaa tcaatctctt taaactatta tatgtattta cacaaagcat ttataacata
    17161 ctttaaaaat agtaccttaa aataagaaac ttaataaata aaaaaagatt acgtggctac
    17221 gtcctactct cccaggcag
//
LOCUS       DAMJWU010000280        16191 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.guided.084, whole genome shotgun sequence.
ACCESSION   DAMJWU010000280 DAMJWU010000000
VERSION     DAMJWU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.guided.084"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            complement(120..812)
                     /locus_tag="PC460_003853"
     CDS             complement(120..812)
                     /locus_tag="PC460_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2602637.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(838..2025)
                     /locus_tag="PC460_003854"
     CDS             complement(838..2025)
                     /locus_tag="PC460_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2602638.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            2361..4238
                     /locus_tag="PC460_003855"
     CDS             2361..4238
                     /locus_tag="PC460_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2602639.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKVGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            4548..5954
                     /locus_tag="PC460_003856"
     CDS             4548..5954
                     /locus_tag="PC460_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2602640.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDIYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            5982..6830
                     /locus_tag="PC460_003857"
     CDS             5982..6830
                     /locus_tag="PC460_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2602641.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNEKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            7332..8024
                     /locus_tag="PC460_003858"
     CDS             7332..8024
                     /locus_tag="PC460_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2602642.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            8021..8956
                     /locus_tag="PC460_003859"
     CDS             8021..8956
                     /locus_tag="PC460_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2602643.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            9104..10027
                     /locus_tag="PC460_003860"
     CDS             9104..10027
                     /locus_tag="PC460_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602644.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            10011..10781
                     /locus_tag="PC460_003861"
     CDS             10011..10781
                     /locus_tag="PC460_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602645.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TVLRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            10791..11573
                     /locus_tag="PC460_003862"
     CDS             10791..11573
                     /locus_tag="PC460_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602646.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            11950..12486
                     /locus_tag="PC460_003863"
     CDS             11950..12486
                     /locus_tag="PC460_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2602647.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            12518..12907
                     /locus_tag="PC460_003864"
     CDS             12518..12907
                     /locus_tag="PC460_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2602648.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            12944..13330
                     /locus_tag="PC460_003865"
     CDS             12944..13330
                     /locus_tag="PC460_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2602649.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            13457..14899
                     /locus_tag="PC460_003866"
     CDS             13457..14899
                     /locus_tag="PC460_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2602650.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            15196..16011
                     /locus_tag="PC460_003867"
     CDS             15196..16011
                     /locus_tag="PC460_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2602651.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         5856 a         2002 c         2971 g         5362 t
ORIGIN      
        1 ttgttgaaat gatgaaaata tatttgattt catacaaaaa agagtacctc ataactaaaa
       61 agttcacttt gagacactcc atttttatat gttagttgca tttaaggtaa aatagaagtt
      121 taatcatgtt gaaataactg aattacatct tctaaatgat tgtacattga tgtataacct
      181 aagtaagagt ctttactatc aaatgcaatc attatcttgt tatggtggct aatcatatcc
      241 ttgagagaat ttctatattt aaaaccaaag tattggttag aagaaactgt tttaggtatt
      301 aactcataat attttataaa aattggatta tgagaagcta aaattatctc tttgtgaaat
      361 tgcaagtcag catcataaca ttcttctatc aaaccgtgag aaaccttatc caattcttct
      421 attgacttta ggtaagtata ttttattttt tgaagttctt tagatgtagc atttaaaatt
      481 gctagtttag tagcttcact ttcgataagt tgtcttgctc ttaaagattc tacataaaat
      541 tcttgtatgt tatgattttt atagatttca ccatcatcaa ataggtcaac ttttttaaat
      601 ccttcaacta taggacttga ataaggcaca gctctaagta ttccttcctt acagagtata
      661 tttatggcat ttcttacagg tattctactt atgttaaata ggtcagcaat ctttctctca
      721 cttggtaatt tactattaat tttaaaagtt ccatttagaa tgtttaattt aatataatca
      781 ataatttttt cagtggcatt taagcttttc ataaaaactc ctaaacatat aattttttta
      841 tgattgtatt atatcatagt taagagttaa atctactttt ttataatatg tataagacag
      901 gatagaggcc aaaatagcta taatagccaa gataaagtat ccatattgta ttgaagtata
      961 ttccgatatt ttaccaacta tcattggaaa tacagacatt cctatagcat aaattgcttg
     1021 aaaaaaaccc attgcagtag atttctgatg ttttggtaca tttttcatag actcgctagt
     1081 aaggtaagaa aggagtatac ctgtagatat acctggtagt acttgtagta caaatattat
     1141 attaatattt tttattgttg gaactaaaaa gcaataggct gaaactaaaa taaaaactaa
     1201 aggaatccaa aattttggcc catacttagt acaaaattta gtagaagcaa agccagaaga
     1261 agcaacagca cacaccatgt aaaatataga agatagacct atgagggtag aactagaacc
     1321 taagttttta agaatttgag tagtaaatga cattgttgta gacatttgaa taccctgttg
     1381 aattaatgcc ataaaggaaa aaaatacaag tcttttatcc ttgcagatag ataaatattt
     1441 tttaattgat attttttcag gtattttttt tgaattatga ttttttatat ttaaggctag
     1501 aatcgttcca ataactgaag acaatacact catgatacat aaggctgtca ttcctaattt
     1561 ttcataaaat aaagtactca taacaaatcc aatcaacatt cctagattgt ttactaatat
     1621 tattgtactt gtagcttttt gttgatgttc tgtagaaaaa aagttggtat agtgaaccat
     1681 gtaagaaatc caagttgatg atgatattcc agataaaatg ttagctataa gaaaaccatt
     1741 gcctccagga gaaaagactc ttataagaga tgctaaacca gcaaagaaaa ttccagacag
     1801 tataaatatt ttatgtttat ttatggaatc agcaaatact ccaacaggaa gccttaataa
     1861 acattgtgag attccatatg cacctattat tataccaatc atatttgtag atacattaca
     1921 tgcacttaaa tatggatttt ggaaaggtat atatacatat tgtgaaaacc agaaaaatgt
     1981 aacaatagaa agtaaaagta atttttgatt agatattttt ttcatattat cctccgtatg
     2041 gtatttttat aatgcaaata atataccatt tggatttttt aatcaagtac tttctctatt
     2101 aaaaaaaatt taaatatata attattattt acaaaaaaat tatactttat agttgaatta
     2161 agaaaacgct tatgatacta taaagttaac aaatttgaat atataacagt agacgtgtta
     2221 ctggtaatgc aggcaagatc gaatcaattt ttatagatta tctatagagt actatgggta
     2281 taatctattt atttggttcg atttttttgt ttaattggcc ataaaacaaa aagcctatag
     2341 ttgaaaatgg aggatttttt atggattata aaaatatagc tcaagaaatt ctactaaatg
     2401 taggaggaaa agagaatgta aatgaagtga ctcattgtat gacaagactt agatttaaag
     2461 tcaaaagcgc ttctaaagtg aataaggata aattatctaa aactgaagga gtaataactg
     2521 ttgttgagag tatgggtcaa atacaagttg taattggaaa taaagtaaaa aaagtatatg
     2581 atgaggttat aaaaattgta cctcaatcaa ataacgtagg taaaaagaat gaaagtcaag
     2641 aaaagcaagg tataattaat tctatattat cagcagtagc tggtatattt acacctacaa
     2701 tacctgctat agcaggggtt ggtatgatta aaggtatttt atcagttctt gctatgtatt
     2761 atatgaataa aaatggagta gatataaaag aaacgcaaag ctatattatc ttaaatgcca
     2821 tggctgattc tatattctat tttatgccta ttatactagg atatactgcg gctaaagtat
     2881 ttaatgcaaa taaaataata tctatggtat taggagctac actttgttat ccaactttta
     2941 ctgctttgat ggcaggtgaa gaatctgtta gatttttagg attggcagta acaaaagcta
     3001 cgtatacatc atctgttatt ccaataatta ttgctatatg ggctctttca tacgttgaaa
     3061 aagtattaga aaagtatata cctgaggtaa ttaaaataat aatggttcca actttatcat
     3121 tggtagtaat gttacctgca acattatttt tatttggacc aatcggaatt tatataggaa
     3181 atgtcataaa cttttcttat aagtatattt atgaattaag cccagcattg tgtggagcat
     3241 ttgtaggtgg gttatggtgt gtgttggtta tatttggtgc tcatagagca ttattaccaa
     3301 taggtataag tgatgttgct caaacaggaa gacagaattt attggcattt gcaggagctg
     3361 caaacttttc acaagcaggg gctgctctag gagtattctt taaaactaaa aatcaaggac
     3421 taaaaacaat ttctatgtct gcaactataa cagctttatt tggtataact gaacctgcaa
     3481 tatacggtgc caatcttaga ttaaaaaagc ctatggtatg tgccgtaata tgtggtgcat
     3541 ttggaggtgc gataatggga atgggtggag cttatggtaa tgcatttgca aaccaaggtg
     3601 ttttaactat acctgtatat gctgaagcag gtgcattagg tttcttaagt tatctaggtg
     3661 gttgtgctat agcatttttt ggttcagcta tttcaactta tctagttgga tttgaagatt
     3721 tggaagagtc ttctaataag gaaaattcat ctataaaagt agaaacgaag gatggtgtta
     3781 ttgatataac ttcacctgta gagggcgaat gtatagaatt gtcagaagta aaggatgatg
     3841 tatttgcttc aaaagctatg ggagaaggta tagctgtgtt acctacaaaa ggagtaataa
     3901 cagcccctac tgattgtgaa gtagcttctt tatttccaac attacatgca ataggattaa
     3961 aattagataa tggagcagaa atgttaatac atgttggaat caatacagta gagttaaatg
     4021 gtaaatactt taccaaacat gttaatcaag gagatttagt gaaaaaaggt gataaactta
     4081 tatcgtttga tatagataaa ataaaaaagg ttggttatga tgtaactacg ccagttatag
     4141 taaacaatac atttgatttt gggcaagtag tttcttgtaa atctagttat gtttcaacca
     4201 atgataatat aatttcatta gtattagctg gtaattaagt taatttgact agagataagt
     4261 aaattgattt aaaaggaaat aagtaaatca acaattaaat acatctaaaa gtaagtttat
     4321 tgcaatgaat tgttaaagaa aataattaat atggagtaaa tctattgcaa tgaagtaaca
     4381 aagaaaataa ttaatataga gtaagtttat tgtaatgaat tgttaaagaa aataattaat
     4441 atagggtaaa tctatttcaa tgaaataaca aagaaaagag ttaatataga gtgaacttat
     4501 tgcaatgaat caacaaagaa aataattaat aaaaaggaga aatatttatg aatacaggat
     4561 ttccaaaaga ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg
     4621 ataaagacaa taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac
     4681 aggcagatac taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa
     4741 tgaaagaatt agggatgaaa acatatcgtt tttcaatttc atgggcaagg ataataccag
     4801 atggtgaagg agagataaac cagaaaggtt tagactttta taataagata atagacaaac
     4861 taatagagtg tgatattgag ccattcgtga ctttatacca ttttgacctt ccatttaaat
     4921 tagtagagaa atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg
     4981 ctaaaatatg ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac
     5041 agaatttaat tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga
     5101 agatagataa aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag
     5161 tcaatgcatg tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg
     5221 taacatatcc tcttacttct aagccagaag atatatatgc agctagaatg aatgataatt
     5281 ttaaagtgta ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga
     5341 agtatcttga aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta
     5401 aaaaagctaa aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt
     5461 tacctgaaga ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt
     5521 atggactatt taaaatgtca atgaatccta acttagaggc ttctgagtat ggagcagcaa
     5581 tagacccatc aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg
     5641 ttataataac agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg
     5701 atgactatcg tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag
     5761 atggtgtaga gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc
     5821 aaggttttaa taagcgttat ggacttgttt atataaatag aactgaccat gaaattttag
     5881 atttaaaaag aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg
     5941 gaatagtaaa ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat
     6001 taaataattc gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga
     6061 aaggcattgg ccataattat aaggccgggt ctgagttaaa tgaaaaagat atagaaaaga
     6121 tttttgttct tcatgatgaa aatataaaaa agagtattat acaattggca acagaaatag
     6181 atgcagaata ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact
     6241 taaaacttat gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac
     6301 gttttaaaga aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc
     6361 caaaagagta tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag
     6421 acctaccaga agaagagatt ggtaatatag caggccattt tataaatgca caacaagata
     6481 atccttatag tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag
     6541 ttcattatca cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa
     6601 tgcatctaaa ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag
     6661 attttatata tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg
     6721 taggagctta tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt
     6781 tgatgattca tattcataag attttaggag aattatgcga aaatagttag aatatcttaa
     6841 tatgctagta atattataaa aaattaaaaa ttaactgcac tttaaatatg aataattgca
     6901 ttacttaagg gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt
     6961 ttttaaacct ttatatcatt acaactttaa tgtttatacg cctgtacttc agatgtatat
     7021 ctacttttta catttctcaa catgcaaatc taccattctg tatatagtaa atgtattatc
     7081 atgtttgttt tattcaagca cacttataat acgcttgatt ttttcacaat gatgaaatat
     7141 actaatgggt gttataggta taagaatcgc attacacttg aagaagtaag tgataaatga
     7201 gatataaatc ctcatatgat aaactactat ttcattgagg aacatattct aagtgatgtc
     7261 aagatactac aatatgacta aattcctagt gataaaaaag tctacgaatg agtgtagaaa
     7321 ggggaactaa gatgaataaa gttttaatta tagatgatga taaagatctt tgtattttgt
     7381 taaaaaagag tatttcaatt gaacagattg aagcaaacta ttgccattct gggagagatg
     7441 gactggttga actttcaaaa caagattatc agttagtagt tctagatgtg atgatgccag
     7501 gtatggatgg ttttgaaact atggaacaaa tacgaaaata tagcagtatt cctatcttaa
     7561 tgctgacatc aaaaagtgat aattacagca aagtgcatgg attgcgagct ggtgcagatg
     7621 actatctaac caaaccattt gaaattacag aatttactgc tcgtgtactg tctttaatcc
     7681 gacgttacac ccgttttaat ccaaaaaatg aggagccaca gcaactttct ttcaaaggtt
     7741 tgttgattga ccttgaccag catacagtta ctaccaatgg cgaacctatg ttgcttccac
     7801 ccaaggaatt tggtgtactt ctttactgtg caaggcatca gggtaagatt ttgacaaaac
     7861 agcagattta tgaagccgtg tggggtgagc catatgtata tgatgacagc aatatcatgg
     7921 ctattatcag tcgcctaaga aaaaaaattg aactagaccc atcatctccc atctatttgc
     7981 aaacaataaa aggaattggg tatcaattca acacggaggt atgaaaatgg atgaacaaac
     8041 gataattctt ttcttactct tgctgtcgat tggtctactt tttctttcca tatttgcttt
     8101 ttccaagctt gggcatattc acaaacgctt aaaagatata gaggagatac tcgctgatgt
     8161 acaaattgga gacgaaaatc gtaaaatact aattaagcca tgtgatgtta tggctccatt
     8221 ggtttatcag cttaatgaga ttgtctacga ttatgaaaat aagttacttt ctctgaaaaa
     8281 gtcggataaa gctagcaaac aacttatgac aagtttatct catgatgttc gtacaccatt
     8341 aacaactctg attggttatc tggatgctgt acacagtggt atagttatcg aacaagagcg
     8401 ggaggaatac cttgaaatcg ctaggcgtag agcctacgac ctcaaggatt acattgatgt
     8461 tctttttgac tggtttcggt tgaactctga tgaatttacg ctgtcaatag aatctgtgga
     8521 aattgcagag ttgtcacgca atattttgaa agactggatt cctatattcc atgaaaagaa
     8581 actgaatttt gaaattgata ttccagaaaa tcgactaatg gtaaacctcg acccagatgg
     8641 atattcccgt gtggtcaata atctggttca aaatgttctt gctcacagta aggctcggca
     8701 aatcaagatt accatgagtg aggattccag aatggtatta ctccgtgttg aggatgatgg
     8761 tgttgggatt gccagagaaa atttaccaca tatttttgag cgactgtata agtgtgataa
     8821 aggacgttca gaaaaaggaa gtggtcttgg tctatctatt gtcagtcaaa tggtggagcg
     8881 aatgggtggt caggtttccg tagaaagtga gattggaaag tataccgtat ttacagtgag
     8941 cttaccatta atataaaatt gtttttcccc catgtgacat gtatacacag ggggattttt
     9001 tgtaagttat aaagttgcaa ggttaataca aggttgttgc aaggttaatg taagattgag
     9061 gtggtatgat aactttaata aacaaaggag gcttttatta tttatgaata atttaattat
     9121 tgaaacaaaa aatctcacta agcaatatgg tgctcaaaag agcgtttcta atttgaatat
     9181 tcacgttcag aaaggtcgca tttacggatt gctaggaaga aacggtgcgg gtaagacgac
     9241 aactatgaaa atgttattgg gcctaacaaa accaactact ggagaaatta aaatatttgg
     9301 tcaggacatt cacaataacg agaagaagct tcttcctcga attggatgtt tgattgaatc
     9361 accagggttt tatcctaatc tgacaggaac agaaaatctc aaaatctttg caaggcttag
     9421 aggaatacca aaaaaaaatg cagtagaatc agcactggat gtagtcggtt tgccaaacaa
     9481 tgacaaaaag ttattttctc aatactctct tggtatgaaa cagaggttgg cgattgctct
     9541 tgccattatg catgacccac aactgttgat tttggacgag cctatcaatg gacttgatcc
     9601 tattggtatt gcagaagtaa gaacgtttat tcgtgatctc tgcacagaac gaggtaaaac
     9661 tattttgatt tccagtcaca ttctttctga aattgccttg ttggcagatg acattggcat
     9721 tattgaccat ggtattttgt tggaggaaga aagtcttgcc caattagaag aaaaaaacag
     9781 taaatacata cattttgctg tatctgatgc agcacaggca gcaaggcttc tggaaaccca
     9841 acttaacctg aaaaatttca aagtagcaga taattacaat attatgctat atgatacaga
     9901 tgtttctgtt gcagaaataa atcgtgcttt cattctaaac aacattgagg tatcagaatc
     9961 tcatttatgc gaagatactc ttgaagatta cttcaagaaa gtaacagggg gtgaaggaat
    10021 tgcttgatat aatttcatgt gaattttcaa aactcaagcg gttaaaattt atactgattt
    10081 ctattttagg tgcttgctta tttccaattc ccgcaacaat tttgattgca aaggacaact
    10141 tgccctttga gcaattgttc aaattagttg tcaattttgg atatttcttg ctattaccaa
    10201 ttgtattaag tattgttgct agccaactat tttttataga aagagataat gatgtattaa
    10261 aaaatcttgc aacagtgcca gtgccaaaag gaaaactggc tctggcaaaa ttggctgtgt
    10321 tgctgtttat atcattgttc tactctgttg cagggttagg agctactatc attggaggtt
    10381 tcattgttgg aatagtagag ggggttgctg taaaattagg aatgagcatt gcttttggaa
    10441 ttatgttttt tgttactgta cttcctgttg tggtactcat tgtatatttc aatcgcagct
    10501 atattttttc tattatactt tcttttgtat atgctatttt caattttagt atatccttaa
    10561 atattatcaa ttttgaacca aataacccac tattaagcgt attacctgct cctgttataa
    10621 tgcgttggtg gatggcattt tggggagatc ccactggcga atacacggta cttagacagc
    10681 catatttgct ctcaactcct gcttgtgtag gtattttatg tttgataacc attatagcag
    10741 tcctgctgat ttgtatgata tacaaaaagc aggagaatta ggaggtatct atgcttgata
    10801 taattactac agaatttcaa aaaataaaaa gatataatat tctatggatt ggtgttgttg
    10861 ctgttctgtt ttcagcacta ttggcagttt ttcaggttgt aagttcacac ggcagtgacc
    10921 ctctaacgta tgagagcttt gcaaacggag ttatctggaa taatttcagt cttagttttc
    10981 catttgggat tacacttatt ggaggatatc ttattaaccg tgaatataca gaccaaacct
    11041 taaaaaacat cctaactgtt cctatttcat tacgcaaatt gctagtggga aaattaattg
    11101 cggttggagg aattactgta ttgttaagtc ttttcagctt cttatgcaca tttattttgg
    11161 ggttgttatt ttgcaatatc aatatatctg ttatactcgt tgtaaaatcc ttagttcaga
    11221 ttttgtctgt taatctgtat tgttatgttg ctgttttgcc gattattgct tattttggtc
    11281 gtaaacaaaa tgcatttctg acgggtgtag ggattgcatt tgtatacggt ttttgtggtg
    11341 tgtttgttgc tggaagaaat ctgacagact tttatccaat cacagcaggt cttggtctag
    11401 ttggatacaa caatggtgct ggtactgttt atcagccact tatcggtgct atgactttga
    11461 ttgtaattct aatcttgaca actattctac ttgtatttac acctaattat gataaagtta
    11521 tgaccatatc taagaaaaaa gggataaagc ataaaaagta ttctcataaa taacataaat
    11581 attgtttcta ttatttaagt tgctagacag tgaaaagaaa aaatctgtcg tacagtggct
    11641 atatgctcat gtattcaaga agctacagtt ttgatttttc ataactgtag cttctttttg
    11701 tatttattaa atcacgacta tcataatact tgcaataggt atagtaacaa gagagagtac
    11761 tgtcatatgt gttttatata ttacattaag tatattctaa aatatatgtt ttaaataata
    11821 tatttgaaaa taggaatatt taaataaaaa aaataatatt atatatagtt gacaaaaatt
    11881 ggcaaatgat atactatata aatatagaaa tagtaatcat tactattatt aaaaagggag
    11941 gaattaatta tgaacttaaa aggaactaaa acagaaaaga atttaatggc aggatttgca
    12001 ggagagtcag aagcaagaaa taaatataca tactacgctt caaaagcaag aaaagaaggt
    12061 tataatcaaa tagctgctat atttgaagaa actgctaaca atgaaaagga acatgctaaa
    12121 ttatggttta aacttttaca tgatggtatg ccttcaacag aggaaaattt aaaggatgca
    12181 gcagcaggtg aaaactatga atggactgat atgtatgcaa aatttgctaa agaagctaga
    12241 gaagaaggat ttgacaagat agcttattta tttgaagctg ttggaaaaat agagaaagag
    12301 catgaagaaa gatacttaaa gcttttagaa aacttaaatg aaggaaaaat atttaaaaga
    12361 gatgaagaag ttgtttggca atgtcaaaac tgtggtcatg tgtatgtagg tactgaagca
    12421 ccagaaaaat gcccagtatg tgaccatcca aaagcatatt ttaatataaa agctgaaaat
    12481 tattaaaaat tatcattatt aaaaaggaat gttaactatg aaattttcta aacaacgaga
    12541 actgatttta aatgaaatat taaataatcc ggttcatcct actgcggatt acctatatga
    12601 aaacttaaaa aaagataatc cgaatttaag cttaggaact gtgtatagaa atttagctca
    12661 gctaacggaa catggcttta taagaaaggt tagtattcca gggtatccag atagatttga
    12721 tggtcgaata gataatcact atcatatcat atgtgaagta tgtggagagg tatacgattt
    12781 agaatccgaa gttcttaata atttacaaga attaatatct gaggagacag acataaaaat
    12841 aacatcttat aacataagtt ttaaagggat ttgtaataat tgtaaaaggt gtagccaagt
    12901 aggttagttt tcaaattcta aaaataatta atgaggtgag tttatgtgta gtgaacaaaa
    12961 attttttata tgtgagactt gtggaaacct tgtaggaatg atacaaagtg gtggtgtacc
    13021 tattttctgt tgtggaaaac ctatgaagga acttgttcct aatacaacag atgcagcagt
    13081 tgaaaaacat gtacctgtaa ttgaagtgga tggaaataat gttactgtta aagtaagtag
    13141 cacaactcat ccaatgacta aagaacatca tatagcatgg gtgtatctta tgactgaaca
    13201 aggtggtcaa cgcaaatgtc tagcagtaga tggagaacct gtagttaaat ttgctttgaa
    13261 tgatgatgat aaagtgattt ctgcctatgc ttactgtaat ttacatggat tatggaaggc
    13321 tgaactgtaa tatttaaata aattgagtca aaaaggaggc tataaatgta ttttcataca
    13381 aatagcctcc tataaattta tataaattca agttttaatt ttgtttaatg tcaatataat
    13441 ataaggagaa aataatatgt caatttataa atgtagtgtt tgtggatata tttatgatga
    13501 atctaagaat gataaaacat gggatgaatt aagtgaagat tgggaatgcc ctgtatgtac
    13561 aaaaggtcgt agttattttg gaaagataag tactgtttac tatgaagaag atgaaaagat
    13621 agcagaagac atagttgaag atgaatctaa attaaatact gaaaaagagg gagatttaaa
    13681 ttacttaagt acatatttaa gaagagatga tgaagttgag aaacatatgg atataattca
    13741 cgaaatggct gttacaggaa aatctataat agaacctatg agaactaaac taccagtaat
    13801 ttcttgggat gatattttaa ttatgggtgc tcaattaaat ccattacctt taaatgagca
    13861 tgatgaagta aatacaacaa caataatagg aaaaaaagca aagaaaccta tgattataga
    13921 aaatccagtt tatatttccc acatgtcatt tggagctttg tctaaagaac taaaaatagc
    13981 tttagcaaag ggtgcagccc aaaataagac agctatgtgt agtggtgaag gaggtatatt
    14041 accagaagag aaggaagctt catacaagta tatttttgag tatgtaccaa ataaatatag
    14101 tgtaactgaa gaaaatctta aaaattctga tgctatagag ataaaaattg gtcaaggaac
    14161 aaaacctggt atgggaggtc atcttcctgg cgaaaaagta actgaagaaa tagcaaaagt
    14221 aagaaataag cctgtaggtc aagatgtaat aagtccatct tgttttgagg aaatacaatc
    14281 caaagaggat ttaaagaaac ttgtagatga acttagagaa gtttcagaag ggcgtccaat
    14341 aggtgttaaa atttcagcag gtcatattga aaaagatatg gaatttatag cttatgctaa
    14401 acctgatttt gtaactattg atggtcgtgg tggagctact ggagcaagtc caaagttgct
    14461 taaagatgca acttcaattc caacaatatt tgctctttat cgtgctagaa aatacataga
    14521 tacacatgga ttagatatag atttggttat aacaggtggg cttcgtatat ctactgattt
    14581 tgctaaggca attgctatgg gggctgatgc agttgctatt gcaagttctg ctcttatggc
    14641 agcggcgtgt caacaatatc gtatatgtgg ttctggtaag tgtcctgtag gagtagctac
    14701 tcaggatgag gaattgagaa aaagattaca tatagaaaat agtgctaata gagtggcaaa
    14761 ctttttaaat gtaagtttag aagaacttaa gacttttgct agaatttctg gtcataaaga
    14821 tatacatgat ttaagtgttg atgatttata tactgttaac tctgaaatat caaattatac
    14881 aaacatacag catgtataga ttgaagtttg aaactattag attttagaca gctataaaag
    14941 taaatgataa atatatttta taaaagagag tgtcttaaaa tgaacttttt agttcataag
    15001 tcactctttt ttgtatgaag ccaaatatat ttatcatttt agcaatgaaa aagaatctta
    15061 tctctttatt ttgagataga ctccttctat ttatgaaaat taaagatatt aaattgtaaa
    15121 atattctgtg atgtcgtact attaaactaa agattctgta ctttaaagta aataaaaatt
    15181 taaggagtga ttattatgga aagattgaag atacaagttt tagtcgacaa taatacttat
    15241 attgatagat actttgttgg agaaccagca gtaagctatt atattgagat tgatggaaat
    15301 aggattttgt ttgactcagg gtattcagat gtattcatat caaacgctga aaaattaaat
    15361 atagatttag gaaatttaac tcatgttgtt ttttctcatg ggcataatga ccatacaaga
    15421 ggtatccagt ttttagaaaa taggtatgat ttatctacag ttgaacttat ttcacatcct
    15481 aactgtttta tacctagaaa acatggagag aaaagcatag gagctccatt ttctgctgag
    15541 gaaattaaaa atatttttat gtataatcct aaggataagc catttaatct aagcaagaat
    15601 tgtgtgtttt taggtgaaat accttctata aatgattttg aaaaaagagc taaaattggt
    15661 aaatgtaaaa taggagattt gtgggaagat gattatgttt tagatgattc tgctatagtt
    15721 tgtaaaactg ataaaggtat ttttattgtt acaggatgtt ctcatagtgg aatttgtaat
    15781 atagtagagt atgctaaaaa ggtatgtgga gatgatagag ttattggtat actaggtggt
    15841 tttcatcttt ttgaattgaa taataggtta gatagtacaa tacaatattt ggataaagaa
    15901 gaaataagaa tgttgtaccc ttgtcactgt gtttctttta aggtaaaagc gaagatgaat
    15961 gaaatattga atataaatga agttggagta gggctgacca taagtatata gtatttgaaa
    16021 gtatataaga tttaagttga aggttaaaaa gatattttct atagaaaata tcttttttgt
    16081 tacgtttatt ttaagagaaa tcaatatact tttatatgaa atattgtaaa aaaatttatt
    16141 tttaatgaga atatattttc attaataaac aattttatta tgtattaata a
//
LOCUS       DAMJWU010000281        15199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.guided.091, whole genome shotgun sequence.
ACCESSION   DAMJWU010000281 DAMJWU010000000
VERSION     DAMJWU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.guided.091"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="PC460_003868"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="PC460_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2602652.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="PC460_003869"
     CDS             1575..2258
                     /locus_tag="PC460_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2602653.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="PC460_003870"
     CDS             2255..4915
                     /locus_tag="PC460_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2602654.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="PC460_003871"
     CDS             complement(5096..6277)
                     /locus_tag="PC460_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2602655.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..6939)
                     /locus_tag="PC460_003872"
     CDS             complement(6277..6939)
                     /locus_tag="PC460_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2602656.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     FLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(7071..7709)
                     /locus_tag="PC460_003873"
     CDS             complement(7071..7709)
                     /locus_tag="PC460_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2602657.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7888..8493
                     /locus_tag="PC460_003874"
     CDS             7888..8493
                     /locus_tag="PC460_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2602658.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            8997..9746
                     /locus_tag="PC460_003875"
     CDS             8997..9746
                     /locus_tag="PC460_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2602659.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            9743..10507
                     /locus_tag="PC460_003876"
     CDS             9743..10507
                     /locus_tag="PC460_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2602660.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            10606..11427
                     /locus_tag="PC460_003877"
     CDS             10606..11427
                     /locus_tag="PC460_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2602661.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKNILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDVVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            11441..13105
                     /locus_tag="PC460_003878"
     CDS             11441..13105
                     /locus_tag="PC460_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2602662.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            13110..14984
                     /locus_tag="PC460_003879"
     CDS             13110..14984
                     /locus_tag="PC460_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2602663.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
BASE COUNT         5710 a         1747 c         2526 g         5216 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtattacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaaggc gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atataaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataag
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggatatag aatcttaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcagttagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctatagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggc tgactgaaaa agatgcatca aaagaacttg cgaaaaatgg
     3901 aggatttaaa agtatagttt catctaaaaa atactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttataa tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtacagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaagtaa
     5701 ttggagtctt aagttgatga gcaatatctg caagattatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatat aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ctgtcctcca ttctatatcc
     6301 tatgcctctt aacgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggactgaa gtactgagag aaaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca tcatcttcta aaagaagtat tttagccatt tacttttaat cctttcagta
     6961 atttagttat tccattaact aagttattag ttttatgttt aatatttttt atattagaga
     7021 tagaaaaaat tatcatttaa gtatataaac tttaatttat aaacttaggt ttatgcttta
     7081 attgaatcta ttaaaagagc aagagttgtt tcaataaatt tttcctcatc tatattaaag
     7141 ttgttcaggt atgattttcc tgttacagat agtaaattaa aaaatcctgt aagtgtaaat
     7201 attgatgaat acataagagt aggtatttca atatcatgcc taatacttct atcatttttt
     7261 ccaatttcaa acattccttg tatttcatca aacataaatt tattaaaaga aaaaaacttt
     7321 tctttaaaca gcatatcagt atccgatttt tcagagttta caataccaat cattcccata
     7381 aggcataata gagaaggttc tacagagtaa aatttatgaa aagcaaagta tgaaagtttt
     7441 attttttcaa accctgtttt gccttttgca ctttctattt taacattatc ccaaagtctt
     7501 ttatatcctc ttaagacaac agcaaagaat aaatcttctt tacatgaaaa gtacttgtat
     7561 atggtgcgtt tagtatattc aacttctttt gctaattcat tcatagttgt attatcaaat
     7621 ccattaagac aaaataattt ttctgcttta tcaataatct cattttctct ttgtattttt
     7681 tctttatctc tttttgataa ttctgacata taaattattc ctttctattg ataaaaaagt
     7741 atactgatgg tatacttatg taaatataat acttgtatac ctatagtata ctttaataaa
     7801 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
     7861 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
     7921 agtactcgtg gctcaaattg ttttattgtg tttgataaag aaattattct tatagacaca
     7981 ggtcttcctt ttatggggaa aaatataatt aaagaattaa aaacacttgg cattaaactt
     8041 acagatatta agcatatttt attaactcat catgatgttg accatatatc taatattaaa
     8101 ttattgaaag aaaaaacagg agcaaaagta tgggctcatg tagaggatat accttttatt
     8161 ataggagaga aggatagacc tgggtttaag aagtttattg gaaaggcaat ttccagaagg
     8221 ctcattaagg atgttgaacc ttatggagag aatatgcaga ttgggaatat aaaaattatc
     8281 catactcctg gtcatactcc agggcatgtc tgcatgattt ttgaagctgt attatttgca
     8341 ggtgatttag tcaaaaataa gaatggaaat atagttcctt atcctaatcc atggaattgg
     8401 gattataaaa agatggtgaa atctataaga gaattggata atttaaaata tgaatggata
     8461 tgtatgtctc atggaactcc ttgtattaaa taataaaaat taacatggat taatagaggc
     8521 atagttgctt gtatgtatga taataattta cagcttcttt ttaatatatc atgtatattt
     8581 gtagctgtaa gcatatttat aacaatactt acaattttgt attttaacat ctataataat
     8641 agttttagct ttgttaacta tgaaatttac ttaaagaagt aaatattcaa gtaatagtaa
     8701 ttattaaata agataaaaag taaatggcag atgaattaaa gtaatttcta ccatggaaaa
     8761 tacttttgag ttagaaaaaa ctatatttta gagtatgaag agatgaagct tccgaagaaa
     8821 gcaatatcta aagtacatgt tcgaaaggat aagtactttt tggtgcctgc ttttagcagg
     8881 ttatttttat tttaataaaa ataaaatcaa atgattattt tatccttatt atttaaaact
     8941 cttccctaga aaaagcaact aatgtatatt aaaataatat ttagggagtg gaaaaaatga
     9001 gattagaatc gttagttaac caaaattatg ataatttaaa tgaaaatgat aaaattatta
     9061 ttaaacaaat attgatttgt caaagagaat accgtaattt ttcgtgtgaa aaaattgcaa
     9121 atgaatgtaa tgtttctaga gctactttat taaggctttg tagaaaaatt ggattagatt
     9181 cattctctga tttaaagtat ttacttaaaa ataatcaact agaatttgtt aataatgata
     9241 ataattttca tgatgtttgc gagaattatc gactattaat gaatgaatta caaaaaatat
     9301 catttcaaga tatttgtaaa ataatatatg atgcagaaac aatttatatt tatgggacag
     9361 gtaatgaaca gaaaagttta gctcaagaaa taaagagaat atttctttca gtaggtaaat
     9421 gtgttattga cttatttgat ataggtgaaa ttgaatttat gagagaatca tttcaaaaaa
     9481 atgatttatt tattatcatt tctttgtctg gagaaacaaa agcaggaatt gaaattgcaa
     9541 aatttatcaa caatcatata catacgattt ctttaactag gcttgataat aatacaattg
     9601 caacactttg tgagtataat ctgtatgttg aaacgcaaaa acttgataca ttacaagcta
     9661 tatcttatga agtcgtagga gcattttatg ctctattaga tttgcttcat atgaattaca
     9721 tagagtatag aaggagaaaa gaatgaaaat tgaaggactt ttcaataaat attataattc
     9781 atttacagca aatgataagt atatctgtga gtgtattctt aatcataaag aagattgtat
     9841 taaactttct atagatgaat ttgcagacaa ataccatata tctacaagtg cactttctcg
     9901 ttttgctcaa aaactacaat tacctggata tagtgaatta agagttatta tacgcttaaa
     9961 tgaaacagaa gtagaatata aaaatcaaag taaacaccaa ttaatggatt gttataataa
    10021 ggtgatagat gatatagaga agaaggattg ttcgattatg tttgaaagaa tgaaaaaggc
    10081 aaatcgtata atcgtctttg gagaaggtta ttctcaggaa agagttgcta aagagatgaa
    10141 aaggattttc ttaccaacag ggaaaattat atatgatgtt tatggttatg atatgattca
    10201 ggcacttaat cattttataa aaacagatga tatgattttc tttatttcat tgaatggaga
    10261 tgcatctaca attattgatt ttgctaaaga aatgagaatg aaaggaattt atatggtttc
    10321 tattacaaaa atgataagta atcctttagc aagattatgt gatgaaaacc tgtatattca
    10381 atctatatct atttctgtag ataaacaact ggactacgat gtaacaacac cttattttat
    10441 tttaattgaa ctgctttata ttaaatacaa gatgtattta gaaagtgaac atattccccg
    10501 aaaatgaaaa tttgtgctaa aaatgaacat attttttaaa agatatcaaa atattgatat
    10561 cttttttttt gaatgctatt atgattttga tagtaagaaa ggaagatgaa attgtacaaa
    10621 ttattaatac ttgatataga tggtacgctt agagatgagg tatatggaat cccagaatct
    10681 gcaaagcatg ctatacgttt atgtcaaaag aatcattgca gtgttgttat ttgtactgga
    10741 agaagtatgg gtacaataca agatgatgtt ttatcattag gagttgatgg atatattgca
    10801 ggaggaggta attacattca atatcatggt gaattacttt ataatcaatc ttttaatcaa
    10861 agattgataa aggaagttgt ttgtttatta aaaaagagag aagttgcttt ttcgattgag
    10921 agtcaggaaa aagttttcat gaatcaaaaa gccaaagaaa tatttgaaac aatgaatcag
    10981 ttgaaaggaa ctaattcttg tataaataaa caacatatcc aagaaaaaat aacatatgaa
    11041 aataacattg aagagtataa gtcacaagat atacataaaa tttgtttatg gtcaaatgaa
    11101 aaggtatttg acgaagtgaa aaatatacta caagataaaa tggaattagc acagagagat
    11161 atttctagtc agtattatga aattattcaa aaagattttc ataagggcaa agcaattaag
    11221 agattacaag aaagattagg agtaactcaa aaagaaacca tatgttttgg tgatggtcaa
    11281 aacgatgttg tcatgtttca agcatcagac gttacaattg caatgaaaaa tagtcatcaa
    11341 caacttaaag atatagcgac atctatatgt gaggacatat tcgataatgg aatttataaa
    11401 gaattaaaaa gaagaaatat catataaaaa ggagtaattt atggaaaaga aatggtggca
    11461 aaaagaaatc gtttatcaaa tttatccaag aagctttcaa gatagtaata atgatggaat
    11521 tggagattta cagggaatta tttctcgttt agattattta aaaaatttag gtattacatc
    11581 attgtggtta tgtccaattt ttaaatcacc tatggacgat aatgggtatg atattagtga
    11641 ttatttggat attgcaagtg aatttggaag tatggaagat ttaaaagagt taattaaaga
    11701 aagtaaagat agaggaatca aaattatatt agatttagtt cttaatcata caagtgatga
    11761 acatccttgg tttcaagaag cattaaaaaa tccagaaagt ccttatcatg attattatat
    11821 ttttaaaaaa ggaaacgatt taccaaataa ctggaggtct gtttttggtg gaagtgtttg
    11881 ggaaaaggta gagaatagag acgaatatta ttttcattct tttggtaaaa aacaacctga
    11941 ccttaattgg gaaaatccaa tattaagaag tgaactttac caaatgatta atacctggtc
    12001 tcaaatggga gtggcaggat ttagagttga tgctattaca ttcatcaaaa agaatttaag
    12061 ttttcaaagt ttagaaagtg atggtgttga tggtcttgtt aaatgtacaa aaacagttag
    12121 aaatcagcct ggaatagaaa aaatgcttta tgaattaaaa gaaaatacgt ttaaaaaata
    12181 tgattgtatg actgttgctg aagcagcagg tattagctat gaccagctag gtgattttat
    12241 tggagaagat ggtttctttt caatggtctt tgactttaaa catgctgact tagatgttgc
    12301 ttctggtagt gagtggttta aaagaatttc atggacaatt ccagatttaa gaaaatgcat
    12361 tttagatagt caaatgtatg ttcaggaaca tggatgggca gctaatttta ttgaaaatca
    12421 tgaccaacca agagctacaa caaagtatct tttgcaatac gaaaggaatg atgatgcagt
    12481 aaaaatgttg ggagcaatgt atttcttttt aagaggaacc ccatttattt atcagggaca
    12541 ggaattagga atgacaaatt ttaaaagaga taccataaat gattttaatg atttatctag
    12601 cattgaccaa tactatcgtt caatagatga aagttactca gaagaagaag cgttagaatt
    12661 tgtgaattta agaagccgag ataatgcaag aacgccattt ccatgggcag ataaagaaaa
    12721 tgcaggtttt tcagatacaa caccatggtt aaaggttatt gaaaattata aagatattaa
    12781 tgcacagagt cagattaaca attcagagag tattttagaa ttttataaaa agatgataaa
    12841 gttgagacaa gatagtgagt attctgattg tttgatttat ggttcaatag aacctattga
    12901 tatagaaagt gatgatatta ttgcgtatag aaggcaatta gatttggtag ttattgattg
    12961 ttattataat ttttctgata aacctgtaga aataagagta gaagaaaaat atgatattgt
    13021 attccaaaat ttagatgata taaatctatt aaataaaaca gtgcgtttga aaccatttca
    13081 tgcagtatta ttgaaagtga ggtagaaaaa tgaataaata taataaaata gcaaatgagc
    13141 ttattaaaat tattggtgaa gataatatta tttctattac acattgtgcc acaagattaa
    13201 gagtgatggt taaagataga gaaattatta atgataagaa agttgaaaaa gttgatgagg
    13261 ttaaaggggt tttctttact tctggacagt atcaaattat tttaggaaca ggaatagtta
    13321 ataaagttta tgcagaagtt gaaaaaatgg gattaaaaac tttatcaaaa aaagaacaag
    13381 atgaattagt taaaaataat gaaactggat ttaagaaagt tatgagaact ctagcagata
    13441 tatttgttcc tattattcca gttattgcag caacaggatt attcttggga ttaaagggtt
    13501 gtttgtttaa tgataatgta cttggattat ttggaatgtc ttctgcaaat attccattgt
    13561 atattcagac acttgtttcg gttcttacag aaacagcatt tgctttttta ccagcaatta
    13621 ttgtatggtc agcatttaaa gtatttggag gaacacctgt tattggactt gttattggac
    13681 ttatgttagt ttccccaata cttcctaatg catattctgt tgctgaccca tcaaatgaaa
    13741 ttgaagcaat tatggcattt ggatttattc ctattgtagg ttgtcaagga agtgttttaa
    13801 cagctattgt tacagcattt attggagcta atcttgaaaa atggttccgt aaacatatgc
    13861 ctaatgtatt agaccttatt ttcacaccat tttttgttat gctaataaca atgttagtta
    13921 ttttgttagg tgtaggccct ataatgcata caatagaatt aaaaatggtt gatatcatca
    13981 gcttattgat tgatttgcct ttaggaattg gaggatttat tattggattt acatatccat
    14041 tagctgttat tactggctta catcatactt atgtaatgat tgaaacatca ctattagcaa
    14101 atacaggatt taatgccctt attactcttt gtgcaatgta tggatttgcg aatattggaa
    14161 catgtctagc atttatgaaa aaatctaaaa ataatcaagt taaacaaact gcggttggag
    14221 caatgctatc tcaacttttt ggtataagtg agcctgtttt atttgggatt cagcttcgat
    14281 ataatttaaa gcctttgatt attatgtgtg catcttcagg tttaggagca gcaatattat
    14341 caattttaca tattcaatct aattcatatg gattagcagt tttaccttct tatttaatgt
    14401 atatctatga tggatataat ttaatcacat acttattagt atcaatattt gtggttgcat
    14461 tctgctttat tgtaacatgt ttatttggag tgcctaaaga agctataaat gaagatgaag
    14521 acgaagaatt agtgtttaat gaaaataatg aaaactttgt gtctccagct aaggggaaaa
    14581 ttgttgcatt agaaaatgta ccagatgaaa cattttctaa aaaaatgtta ggtgatggtt
    14641 ttgctattga tattatagat ggaaaaattg tttctcctat ttcaggtaaa ttagaaacag
    14701 tggtttcaag tggacatgca tttgggatta aaggaactaa tggagaagta ttaattcatg
    14761 ttggaattga tacagttgca ttaaatggag atggctttga tgttgctgtt aaacaaggag
    14821 atatggttaa acagggagac gttcttgtta atgttgattt aaagagaata catgaattag
    14881 gaaagtctac tttgacaatg gtattgtttc cagatggtaa gaaggtaaat attttggata
    14941 taaacaagga tgtaaaaata ggtcaaagaa tatgtgttga gtagtaggtg atatgtaatg
    15001 actacaaaag aagattatca agagtaattc aatcttttta aagtatgaga aataattcat
    15061 aaactctaaa acaattaaag aggttgtttc aaaatagaga ttgtattgca cccaaattgt
    15121 tgaaatgatg aaaatatatt taacttcata caaaaaatta aatagtggca acaattgagg
    15181 atgtgaggga gagttgcca
//
LOCUS       DAMJWU010000282        10280 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.guided.120, whole genome shotgun sequence.
ACCESSION   DAMJWU010000282 DAMJWU010000000
VERSION     DAMJWU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.guided.120"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            227..844
                     /locus_tag="PC460_003880"
     CDS             227..844
                     /locus_tag="PC460_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2602664.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            952..3483
                     /locus_tag="PC460_003881"
     CDS             952..3483
                     /locus_tag="PC460_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2602665.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3917..4618
                     /locus_tag="PC460_003882"
     CDS             3917..4618
                     /locus_tag="PC460_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2602666.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4668..5750
                     /locus_tag="PC460_003883"
     CDS             4668..5750
                     /locus_tag="PC460_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2602667.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            6011..7111
                     /gene="vanG"
                     /locus_tag="PC460_003884"
     CDS             6011..7111
                     /gene="vanG"
                     /locus_tag="PC460_003884"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2602668.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7108..7914
                     /locus_tag="PC460_003885"
     CDS             7108..7914
                     /locus_tag="PC460_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2602669.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7932..10070
                     /gene="vanT"
                     /locus_tag="PC460_003886"
     CDS             7932..10070
                     /gene="vanT"
                     /locus_tag="PC460_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2602670.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         3904 a         1076 c         1847 g         3453 t
ORIGIN      
        1 ttcatgaggc actctttttg tatgaaatca aatatatttt tattatttca acaataaaaa
       61 agaggtctgt ttctattttg agacaatatt atttttatgt agatttaatc ttaatgagta
      121 taaagtttta aaactactaa aataggagga attttaatat aaaagggtaa ataaagggtt
      181 tagagggcat aatatagaat aaattaatta tttaaggagg gtttttatgt gcaaaaaaat
      241 gtatttatta gtggtaggta tacttatagc aggtttatta actgcatgta atgggagtcc
      301 aactaaggat tcagaacaag cacaagataa tacaaaaaag gaggcaaatg tatcaaataa
      361 cgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa caagatttta aagtgccata
      421 cacagatgca ataaacatat ttaaagacaa atacaaagat gctgatatag tagatttaag
      481 tcttgagaga gatttaaata aatttgttta tactgtggaa ggtgtagatg ataataatga
      541 atataagatg aaaattgatg ctaatacaaa agatgtatta gaagataaaa cagaaaagtt
      601 ggattcagaa gatttaaatg gagtcgctag aaaagaaaaa ttagatttta atgacataat
      661 gacacctcaa caagctatgg aaattgcatt aaaagaacaa aatggaatgg ttaaagagtg
      721 gagtttagat aaagatttag atgttacttt ttataaaata agaatagata aagataaaaa
      781 tgaatatgac ataaaagtag attctaaaaa gggaactgta ttgaaggtag aaaaagaaga
      841 ttagaagatt taaactatct ttaaaataat ttcttttttg aataaaattt gtttaaaata
      901 taacaagtag ggtataaaga gactaaataa ctattttagg aggagtacgg tatgtcattg
      961 aaatcgctaa aaataaaaga aaatttatat tgggtaggtt cgcttgaccc tgatttaaga
     1021 gtatttgaca taattatgta tacaccttat ggaactactt ataattcata tgtattaaaa
     1081 ggaacagaaa aaacagtttt atttgagaca gttaaagata agcattttga caattatatt
     1141 gaaagattaa atgatttaaa tatagatttt gaaaaaatag actatattgt tgtaagtcat
     1201 actgaaccag accatgcagg aagcgttgaa aagcttttgg atttagctaa aaatgcaaaa
     1261 gtagtagctt cagaaacagc aattaaatat cttaaggaaa tcgtaaacaa agactttgaa
     1321 tatgttgcag ttacagatgg agatacatta tcaattgggg acaaaacttt agaatttttc
     1381 tctgtaccta tgcttcattg gccagatacc atatacacat atataaaaga ggataaaact
     1441 cttgtaacat gtgattcatt tggtagtcac tatagtaatg ataagatagt aaatacactt
     1501 gatgaagatg aagaaaagga ttacttggat gctttgagat actattatga ttgtataatg
     1561 ggaccattta aaccatctat ggtaactgcc atagaaaaga taaaagattt agatatagat
     1621 actgtatgtc caggtcatgg tccagtatta actgaaaatc ctagaaaaat tatagacctt
     1681 tattacaact ggagtgtaaa tgaacaaata aagttagaaa aagaagttac aatttgttat
     1741 gtttctgcac atggatatac aaagattatg gcagaagcta taaaagcata tattgaaaaa
     1801 aatagcaact ataaagtaaa tttatttgat gtaatagagc ataagcaaga agatattttg
     1861 gcaaaaatag cagtatcaca aggtgtatta tttggaacac caacaatatt aggagatgcg
     1921 ttaaagccta tatgggatat attaatatcg ttaaacccag ttcttcatgg tggaaaagtt
     1981 gcttcagtat ttggttcata tggatggagt ggagaaggta tagaaaatgc tatggagaga
     2041 ataagtcagc ttagaatgac agcagtaaaa ccttttgctg ttaactttaa accatcaaat
     2101 gaagagattg ataaattaca ttcttataca ggtaaattcc tagataagtt aaattctact
     2161 tttggaagta aaaagaaaac taagaaattt aaatgtgtaa tttgtaatga agtttttgaa
     2221 ggtgatagtg ctcctagtgt atgccctgta tgtggagcta aggaagacca atttatagaa
     2281 gttgaagaag atgaagttac ttttagaaaa gatactgatg aatatttcgt aatagtagga
     2341 aatggagcag ctggttttta tgcagcagat gctataagaa aaagaaataa aacttgtaag
     2401 ataactatga tttctaatga agatgagttg acttactata gacctgcact ttctgatggt
     2461 ataaatgaag aacttggttc agatttctac atggaagata aagattggta tgataaaaat
     2521 aatatagtag taatattagg aacgaatgta gataaattag atgaagtgaa taaaactatt
     2581 atagtgaatg atggagctat taaatttgat aagcttgtaa tagctacagg aagtagaaac
     2641 tttataccac caattaaggg tcatgattta gaaaatgtgt ttactcttag aaatataaaa
     2701 gatttatata gtgtaaaaga agcactagaa aaatctaaga aagttgtagt gattggtggc
     2761 ggtttattgg gacttgaagc tgcatgggaa tttagactaa aaggattaga agtagtagta
     2821 gttgaagcca tggatagcat actatcaaaa caacttgata aagaaggcag taaaatatta
     2881 gaacaatgtg taagagatac aggaatagat gtaagattgg gtgtagcagt tgatggtatt
     2941 gaaggtgatg gaaaagctca aaaagttgta tttaaagatg gagatagtgt tgactgtgat
     3001 atggtagtat tttcaattgg tgtaagagca aacactcaaa tggttcagga tacatctgta
     3061 aaaattgaca gaggtattgt tgtagataag acattacaga caaatgttaa agatatatat
     3121 gcttgtggag atgttgcaca agtgggaaat attagtttag caatatggcc atcttcagta
     3181 gaaatgggaa aaatagcagg ggcaaatgca agtggagata atttgacatt tgaaagtgaa
     3241 gtatatccag tttcattaga tgcaatgaat gtaaaagtat ttagtattgg taatatacaa
     3301 aattttgata aagaaatttc atcaaaagat gaaggtcaaa gaatatataa aaaattattt
     3361 atgaaagatg gttcattagt tggagctata cttataaatg atttaagctg tactgttaaa
     3421 ttgataagat taatatctga aaaaggtgat tttgaggata ttatgaaggc tgatatacta
     3481 taatttaaat acattaaaat aaaaaggagt tgtcttaaaa ttgatttttt tctaatcaaa
     3541 ttttaaattc aactcctttt ttgaattttt atataaaata acatggaaat tttagtaatg
     3601 tctgtggtta catacatagg tttaattgtt ttttatatgg attgtctctg gtataataaa
     3661 ttgaattgtg tttgactgat agatattttt attgaaagtt aatacctgtt atgcgataat
     3721 aaggacgtgt taggggagtt tttataataa caattaatct cagtttttgt gttccaatag
     3781 tagcatcaac acattctgat gtagtagttc ttttcttata aaaattagtt acaaatatgc
     3841 ttgtaattat ttgatatgta aatttaccaa tattataaat gatgacctcg ataataagga
     3901 gagtgaaaaa taatctatga acgaaaatat tttgattgtg gatgatgaaa aagaaatagc
     3961 agatttaatt gaagtatatt taaaaaatga aggttttaca gtatacaagt tttatacagg
     4021 taaggaagct ctagaatgta ttgaatctaa aaatttggat atggctattt tagatattat
     4081 gcttcctgat attgatggat ttcatatttg ccagaaaatt cgagaaaaat actattatcc
     4141 tatcattatg ttaacagcta aaattgaaga tatagacaag ataatgggtt taaccatagg
     4201 tgctgatgat tatataacaa agccatttaa ccctttggaa gttgttgcaa gagtaaagac
     4261 acaattaaga agatatatgc gatataataa ttcttatgaa caacagtcga taattgttaa
     4321 tgagtatgat attaaaggat tgattatcaa taaagaaaca cataaatgta gtttatttgg
     4381 gaaagaagta gcattaacac cgattgaatt ttctattctt tggtatttat gtgaacatca
     4441 aggaaaagtg gttccttctg aagaattgtt tgaagctgta tggggagaaa aatatcttga
     4501 taataataac acagttatgg ctcatatagg gcgattacgt gaaaaaattc atgaaccttc
     4561 taaaaacccg aaatttataa aaactgtatg gggagtaggt tatacaattg aaaagtaaaa
     4621 atattaaagt caatcctgac tttactcaag tgagaaataa aatttttatg agaacagctt
     4681 taatggcatt tatagccatt attttcatat ggattttata ttcttttata ttgtatggta
     4741 agttttctat ctggatagta tcattctttg aaaatgcttt tcgtatggaa tataaagctg
     4801 cattgaattt atatcagcag attttccgta atagtatgga gctcttcatg atactagcta
     4861 tggtgattgt gttttttata atattccgtg tttatttaaa ttggtttaca aaatatttta
     4921 gtgaaataaa tcaaggtatt gactctctta tcaaagaaga tgttggagaa gtggctttat
     4981 ctccagagtt attggcaata gaaaagaaaa taaactcaat taaacatata cttgaacaac
     5041 gtaaatttga gacacaaatg gcagaacaac gtaagaatga acttattgtt tatcttgcac
     5101 atgatttaaa aacaccactt acatcagtta tagggtatct gacactactt cgagatgaaa
     5161 gtcagatttc tgaagaactg cgaaaaaaat atttatctat ttcgttggat aaggcagagc
     5221 gtcttgaaga tttaattaat gaattctttg aaattacaag gtttaattta tcaaatatat
     5281 cacttgaata cagtacagta agcttaactc gtatgcttga acaactaaca tatgagttta
     5341 aacctacttt gatgagtaag aatttaaaat atacactgaa tgttacacca aatatgatga
     5401 taaggtgtga tgttaataaa atgcaacgtg tatttgataa cttattacga aatgctgttt
     5461 attatagttt tgaagatact actattgaaa taacagctgt acaggaggat gattatataa
     5521 agcttaaatt tataaatcat agcaatacga ttccagagga aaaacttgag cgtatttttg
     5581 aacaatttta caggttggat acatctcgta attcaaacag tggtagagct gggttgggac
     5641 ttgctatagc taaagaaata gtggagcttc ataacgggac aataacagct catagcgaga
     5701 acaatataat tacctttaag gttattattc cgattgtagg aaatttgtaa gaatttaagt
     5761 ataaaaaaat aggaaattct ttaggataaa taaataaaga gcactcttgt tattgatata
     5821 attactgtat caaaacaaga gtgcttttat tttgatacag taattatatc aatagaaaaa
     5881 tgtgaaaata tattatgtgg aacagatgca attgtaagtg aaggttgatt ggatttgaaa
     5941 tagaaaaagt attttaacat gtttctgtag attgtataaa aataaataaa tcaacaaagg
     6001 agaatcaaag atgaataata aaaaaattgc agttatattt ggtggaaact caacagaata
     6061 tgatgtatca ttacaatcag cttttgcagt acttgagaat ttgaatacgg aaaaatatga
     6121 tattatacca attggaatta caagggaggg taattggtat cattatattg gagattataa
     6181 taatatccag aataacactt ggtttgaaag ggatactgat ataattccag ttattgtttc
     6241 gcagaaccgt gtcaaaaatg gtattattga atttttaatt cctgatgctg tttctcagaa
     6301 tcatattcca aatagtgcta ttgaatcatg tgaaaatgat attaaataca ctaaacttga
     6361 ttttgtgttt cctgtactgc atggaaaaaa tggagaagat ggaacagtgc aggggttatt
     6421 tgaattggca ggtatacagg ttattggatg tggtactctt tcttcagcat tatgtatgga
     6481 caaagacaaa tcacataaac ttgttcattc agaaggtatt aaagtaccaa aatcaatatt
     6541 actaaaatac tcagataaat ttaatgtggc tgaactgtca aaagacttaa cttatccatt
     6601 atttataaaa cctgtgcgtg caggttcatc atttggtatt agtaaaattt ataaaaaaga
     6661 agagttacaa gtagcagtag acttggcttt tgaacatgat aatgaagtaa taattgagga
     6721 aaatattgat ggttttgagg tagggtgtgc agttttagga aacaatgact tacttgttgg
     6781 aagagtagat gaaatagaat tgtcaaaagg attttttgat tatacagaaa aatatacatt
     6841 gaaaagttca aaaattcata tgccagctag aattgatttg gattgtgaga aaagaataca
     6901 agagacagca aaaattattt atagaactct tggatgttct ggatttgcta gagtagatat
     6961 gtttttaact ccatctggtg aaattatttt taatgaagtc aatacaattc ctggctttac
     7021 ttctcatagt cgttatccaa atatgatgaa aggtattgga ctgtcttttg aagaaatatt
     7081 ggacaagata attgaggtgt acataaaatg atgaataata ttttattaga taaaaataaa
     7141 gaagataaaa tgacaaatac tatcttattg actaaagata aagtttatga tgggaatcta
     7201 attctagtaa atgctttttt accagttaaa acatctgaag atatagattt aattcctgta
     7261 gatacaaggt ttccaagtat tcttatgaaa cgtgaagcta caaacatttt acaaaatatt
     7321 ttaaaaagta tatgtggaat aaatgaaata gtacctgtaa gtggatatcg tactgcagag
     7381 gaacaacaag atatatattc aagttctctg cgtgacaatg gaaaagactt tactaaaaag
     7441 tttgtagcac ttccaaatca cagtgaacat cagacagggc ttgctattga tttagggtta
     7501 aagaaagaag ttatagattt tatctgccct gaatttcctt ataatggtat ttgtaatgag
     7561 tttcgtaagg cagcactaca ttatggtttt atagagcgtt atcagtgtaa aaaagaagaa
     7621 ataacaggaa tatctcaaga accatggcat tttcgttatg tgggatatcc gcattctgag
     7681 attatcaata aaatgaatct cgctcttgaa gaatatatag aatatattaa gagattttcg
     7741 tatacaaatt gtttaaaaat agaagataaa gaaaaaatta tagaaatctt ttatgtgcct
     7801 attcatacta aagaagaagt gagaatctct atacctgaac aatgtgtata tcaaatatcg
     7861 ggtaacaata aggatggttt tattgtgaca ttttggagaa atagaaagga ctaagaaatg
     7921 gaggaacaag aatgattagg aataaatctt atgcagggct tgattatttt aggattatag
     7981 ctgcattact tataattgca attcatacat ctccattatt atcatatagt aaaatggctg
     8041 actttgtgtt aactagtata attgctcgta ttgctgtgcc attctttttt atgctatcag
     8101 gcttttttct aatttcaaca tataattata attgtaataa actgaaatct tttctaaaaa
     8161 atgtattatt tatttacggg atagctattg taatttatat tcccttaaat atttacactg
     8221 gatatttttc catgaataat ttagtgccca atattattaa ggacattgta tttgatggga
     8281 ctttatatca tttatggtat cttcctgcat caattattgg tgcaagtatt gcatactttc
     8341 ttgtaaaaaa actaggttta aagtactcac tagtaatcac tattgttatt tatgtaattg
     8401 gtatgtttgg tgatagttat tatggttttt tagaaaaaat accactatta aagaatttat
     8461 atgggtatat ttttgaagta tcagattata cacgaaatgg tatatttttt gcacctgtat
     8521 ttttcatact tggtggaata atatctaata aatctaatta tatgtcaact aaaaatagct
     8581 tgattggatt tcttatatca ttcttactta tgttatgtga gggaatgttt ttacataaac
     8641 taggggtaca gcgtcatgat agcatgtata ttatgttatt gccatgtatg tatttcttat
     8701 ttacagcctt aacatttttg aaaggttctc gagctaggtt attgcgaaca tcatcattaa
     8761 ttatttatat tatacatcct atgatgattg ttgttattcg tatgttttct aaaatattcg
     8821 gaatacaaac tgtcttggtt gacaatagcc ttattcatta tatagtggta agtatagcat
     8881 cttttatagc ttctataatt attatattga ttcttaaacc tctaaaaaaa gacttgaaag
     8941 gagaacataa ggtaaatacc aatagagctt ggattgaaat tgatttaaat aatttggaac
     9001 ataatgtaaa gatgatacaa aaagctatgc cagaagattg tgaattgatg gcagttgtaa
     9061 aagctaatgc ctatggtcat agtgcttttg aagtagcgac ttgtgcaaac caaataggtg
     9121 taaaagcatt tgcagtagca acaatagatg aggggattga tttacgtcga tatggaatac
     9181 taggagaaat attgattctt ggatatacta acccaataag ggcaaaggaa cttcataaat
     9241 acaatcttac acagagtgtt attgacctta atcatgctat ctctttaaac aaacagaaat
     9301 acaacataaa atcacacatt aaaattgata caggtatgca cagactgggt tttgatgtta
     9361 atgatgtgag gtcaatttta aaggcattta aattaaaata cttagatata tgtggcatat
     9421 ttacacactt gtgtgtttca gatagtctga aagatgaaga tgtagacttt acaaataaac
     9481 aaattgaatg tttttataag ttaattgatt ttctgttaaa aaaaggtata aaaattccta
     9541 aagtacatat tcaaagtagt tatggttttt ttaattatcc caatcttaag tgtaattatg
     9601 taagaattgg aattgcatta tacgggatat taagttttcc aaatgatacg actaaattgc
     9661 aacttgattt acgacctgtt ttatccttaa aatcacaagt aattcttatt agaaaaatac
     9721 aaaaagatga tagttttgga tatggaagag tctttattgc aagcagagat agcgtaattg
     9781 ctatattatc tattggctat gccgatggta tacctagaaa tttatcctgt ggaaaaagtt
     9841 acgtaataat aaatggttgt agagcagcaa tagtgggtcg aatttgcatg gaccaacttg
     9901 ctgttgatat tacagatata cctaatgtag aagttggtaa tacggcaata ataataggaa
     9961 gagataattt atctgaatta tctgtttctg aagttgcaaa taactcatgt agtatatcga
    10021 atgaattatt gagcagagta ggaagaaggt taaatgttat aaagaagtaa ctcaattaaa
    10081 tagtaatatt tgttgtactg tgtaatttat tttactctga ctagacattt ttagtaatat
    10141 tatgtctaat atatattttt tataacttac atgacaattt aagttataaa aaggtgatgt
    10201 ttcagatttt tattttgaaa catcaccttt tttgttataa tatataataa aataattata
    10261 ccttaactgt aaacttatat
//
LOCUS       DAMJWU010000283         3355 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49302 isolate JH325
            SAMN32366167-rid16592193.guided.204, whole genome shotgun sequence.
ACCESSION   DAMJWU010000283 DAMJWU010000000
VERSION     DAMJWU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366167
            Sequence Read Archive: SRR22862990
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560846.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,807
            Genes (coding)                    :: 3,762
            CDSs (with protein)               :: 3,762
            Genes (RNA)                       :: 81
            rRNAs                             :: 9, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 22 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 9 of 45
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366167-rid16592193.guided.204"
                     /strain="NR-49302"
                     /isolate="JH325"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            248..964
                     /locus_tag="PC460_003887"
     CDS             248..964
                     /locus_tag="PC460_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2602671.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            939..3272
                     /locus_tag="PC460_003888"
     CDS             939..3272
                     /locus_tag="PC460_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2602672.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
BASE COUNT         1410 a          297 c          570 g         1078 t
ORIGIN      
        1 atttaaatct ttagaaaatc tttagaaaag tatatgtaat atcttaagtt tttaatttta
       61 atattaagta tataaaacaa aatgaaaaaa tattaaattt aaactagaag gaggtttcta
      121 agatggacat taaatacaaa taaccagaac aaaagatata aatatatata taaatggaaa
      181 aacttctatc atattgtata atcatatatg taaagaaaat ttataaatta atataaagga
      241 gattagaatg aattcatata atattttggt ggtagaagat gaaaaggaga tagcagatgc
      301 tatagaaata tatctattaa atcaaggata taatgttttt aagggataca atggattaga
      361 aggtcttaag gttatagaaa accaagaaat acatttagct atagttgata taatgatgcc
      421 taaaatggat ggtataacac ttacaatgaa gcttagagaa aactataatt ttccagtaat
      481 aatgctatct gcaaaatcag aagaagttga caaaataatg gggcttaata taggagcaga
      541 cgattatgta acaaaacctt ttaagccatt agaactattg gctagagtta attcacaact
      601 tagaaggtac actaagtatc taaacatggt tggaaataaa gagcaaaagg tagaaagtga
      661 cgatagtgtg tttgcaatag gcggattaga attaaatgaa aatacaaaag aagttagtat
      721 tgatggaaag tatataaaaa caactcctat agaatttaaa atactaagtt tacttatgag
      781 aaatgcagga agagtttttt ctgctgatga aatatatgaa agagtttgga atgagaatgc
      841 tgtaaataca gatacagtta tggtacatgt gaggaatata agggaaaaga tagaaattga
      901 cccaaaaaat cctaaatatt taaaggtggt gtggggtgtt ggatacaaaa ttgaaaaaat
      961 ttaaagataa atatatagtt actattatag tttttatact tatgttatca gcatcattag
     1021 gtatgattag tcaatattct actatacaag caagtgctaa aggtggagca gagaatccat
     1081 ttgaacagga aagctttgta gatagtatat ataaaggaag ttatgtttta tatcataata
     1141 tgaaggaaga acaagaaggt aagatgatac aaccatcaaa gctatttatc aatgaagaaa
     1201 ctatgaacta tataaaaaat gtaagtaaag aaaaagatac ttatgatggt tcagaaactt
     1261 attatacaga tgatagtatt aatgcagatt ttaatgaaaa ctttgaaaat tgggagcatt
     1321 tcattaaagc gtcaggaaag aatttaaagt actatttagt tgataaagaa agcaattttg
     1381 aattttttaa tgataataaa gaattaggag tacttgatac aacaaaaaat gatgcttcaa
     1441 aagaaaattc taatgaaaat cagtcaaaaa atacagaaat gaatgatgag gaaagacaaa
     1501 agcagattac agatataaaa gaaaactata gattctacgt tgtaatgaat tttgataaag
     1561 atggaaaagt aaatattgtg gattcttatg gagtaaatca aaaaattatt aatcaaatgt
     1621 tgttatctaa agcaagagaa gatttaatgg atacaagttt aggaggaaat tcaatttcat
     1681 ataagttatc tccaataaaa aatataacat tagtatatgc tgtacctaaa gtaataagta
     1741 aaggtgatag tgtagagtat ggttatggaa atacttattt tgatgatggt ataatttcat
     1801 atataagcaa tgtagaaaat aattcttatt ataagatttc tggaatgatt ttaaaaatta
     1861 tattagctat agttgttatt gtagctttag cattcccata tagaaaatct aaagagttgc
     1921 taggatttga tacattatca agaataccgt tagagatact ctttattgct cttgctatta
     1981 tacaggggct tgtagattta tatcctactg ttataataag taataccttg agtgggaatt
     2041 atgtagagta ccttataaac aatgacataa gtgctaaaat ggctagtata ttggtaagtt
     2101 taatgaattt aacttattgg tttgtaatat tctcaataat atttgtattt ataatacttt
     2161 taaaacacat tttaaatgtt ggaataagag agtattttac aaaacatact gtgacaggaa
     2221 tgttttttgt atggttaaaa aaagcaggaa aagcagttat taatcaaatt agaacaataa
     2281 atctgaaaga aaagccaaat aaaactatag ttacaatttt gggaataaat ttattgatta
     2341 tagttataat gtgtagtatg tggttctttg gaataatact cgcattaata tattcagtag
     2401 tattatttaa gatactgtca gattactcta aaaagacaat atatgaatat aatcaacttt
     2461 tagatgttac aaagaaaatt tcagatggaa acttagatgt aaatatggaa aaagatttag
     2521 gattttttaa tcctataaaa gatgaattag gaaatattca aactggattt aaaaaagctg
     2581 ttgacgaaga ggttaagagt caaaagatga aaacagagct tatatcaaat gtatcacatg
     2641 acttaaaaac acctctaaca tctataatca cttatataga tttattaaaa gatgaaagta
     2701 taacagatga aaatcgtaag ttgtatattg atacattaga cagaaagtca caaagattgc
     2761 aacatctaat agaagatttg tttgaagtaa gcaaggcaaa tagtggagat gtttatttga
     2821 atatagtaaa tgtggatatt gtatctctaa tgaagcagac acttttagaa ttagatgaca
     2881 aacttgcaga ttcatctcta atagtaaaga ataacttttc taatgaaaaa ataatattac
     2941 cattagatag tcagcgtaca tttagagtat ttgaaaatct tataataaat ataagcaaat
     3001 atgcaatgcc aaattcaaga gtgtatattg atattttaga aacagataat aaagtaaata
     3061 ttatgcttaa aaatatgtct gcatctgaga ttgattttag tgtagatgat ataatggaga
     3121 gatttgtaag aggagataaa tcacgtaaca cagaaggttc tggtcttggt ttagcgatag
     3181 caaaaagttt tatagaatta cagggtggta agatgaatat aagtgtggat ggtgatttat
     3241 tcaaagttac aattactttt aacaaaaatt agattagact tctttaataa ttataaagtc
     3301 tatagaataa aagaatctgg aaatatttat gtgtattctt taaataaaaa tttat
//
